LOCUS       DAOPYX010000001        30010 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_1506_length_30010_cov_8.492572, whole genome shotgun sequence.
ACCESSION   DAOPYX010000001 DAOPYX010000000
VERSION     DAOPYX010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 30010)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 30010)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30010
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1506_length_30010_cov_8.492572"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1497
                     /locus_tag="PKN55_00005"
     CDS             <1..1497
                     /locus_tag="PKN55_00005"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012913.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase domain-containing protein"
                     /protein_id="HNX69654.1"
                     /translation="PAAEFAAACQRLLGPWLATRHAGLQLDFPVDLRFPGSASGTLDS
                     TNLQPTDWLRMARWVLAHYTEYDAFVVLHGTDSMAHSGAALSFLLNGFAADGQLSAAL
                     SRPVLLTGSQLPLFAQSAAGLTLNAQGDALANLDGALHACQRGVCEVAVYFQRALWRG
                     NRVLKTDAQAFAAFAAPNDLPLIEDGLECRVHGERFLPPPASPARSLESPAARAAQLA
                     LLDALQTRLAAHPVWTLLAQPADGQANPLAQAIHAAVAAGARGLLLLGYGAGNFPSGD
                     ASEPARGAVYQALAQARQQGVQLVGGTQTPHGGVNGQIYAAGAWLAQLGALNPGDMTP
                     VAAQVKLSVLLALAELHGWSAAQVAMLFQRALLGEMRDATLLDSRRGGVLRPGEALLS
                     QDGGASVSLSLDEGLRLCAADGRLLWQAPGSAQASLLRLGLDGALSLLGRSGEILWAT
                     PPLGGAAAWLRLDGQCADGSLTLRLGDAGADIGRVLWSAADGQGALER"
     gene            1721..2272
                     /locus_tag="PKN55_00010"
     CDS             1721..2272
                     /locus_tag="PKN55_00010"
                     /inference="COORDINATES: protein motif:HMM:NF017015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HNX69655.1"
                     /translation="MHALLAHARLMADYNQWINTRLYAAAAELPHDMLTQDRGAFFRS
                     VLGTLNHVMVGDRVWLSRFARHPARHAALESFRRMSAPQRVDVMLHDTLPELTLARHQ
                     LDQTIIDWCTELTADALAHPLAYQSLKGVPAVRPMGSLMMHFFNHQTHHRGQVTTLLS
                     QCGVDIGETDLLGLIPQCDEEPV"
     gene            2269..2790
                     /locus_tag="PKN55_00015"
     CDS             2269..2790
                     /locus_tag="PKN55_00015"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNX69656.1"
                     /translation="MSAFAARAATLADLPVVCSWVDDADTLFYLHPRAQFPLTVAQLA
                     EAFAARALNTVLLADGAPVAFANCYRHTPGECAMIGNVIVAPAWRGRGAARALLAAMA
                     QQAIARWQVRELGVSCFNGNVAGLLCYPALGFVPHAVEARQRDGQPVALLHFRASAAA
                     LAATVEPCSPPAV"
     gene            2821..3525
                     /locus_tag="PKN55_00020"
     CDS             2821..3525
                     /locus_tag="PKN55_00020"
                     /inference="COORDINATES: protein motif:HMM:NF016300.2"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HNX69657.1"
                     /translation="MRFSVLAPLALASVLFSAGALAEPLNYNQIQLGADARREINNDT
                     LNAVLFTEDSHASPARLSAVLNERMAAALKIASQHGKVKASSGQLSTWPVYDNNQRVT
                     GWRGRAELRLESRDIEQASQLIGQLQNQLQIAQVGFSVSDEARRKVESELIPQAIGAF
                     RDKARLIVQALGGRDYRLVEMRVDQAGQPGLQVFRAQRAMKAGAPMMAAEAAPSFAPG
                     VSEISVQVQGTVQTAP"
     gene            3637..4248
                     /locus_tag="PKN55_00025"
     CDS             3637..4248
                     /locus_tag="PKN55_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018151066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HNX69658.1"
                     /translation="MKILSSLTSPYGRKARIVLLEKKIDCQLDVDIPWDEDSQVPEYN
                     PLGKVPVLVLDDGTALYDSRVIVEYLDKLSPVSRLLPAESRPLAMTKRWEALADGVID
                     AAVAIYLEKQRESTQQNPGWIERQFGKMRRGLAAMSADLGEKTWCNGESYSLADIAVG
                     CSLNYLDLRFAHMNWREQYPNLAALMAKLEARQSFIDTRPPVL"
     gene            complement(4396..5043)
                     /locus_tag="PKN55_00030"
     CDS             complement(4396..5043)
                     /locus_tag="PKN55_00030"
                     /inference="COORDINATES: protein motif:HMM:NF025252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HNX69659.1"
                     /translation="MKEPYHLAQRLLRRASQGALATLSAELDGWPFASAVNLVALADG
                     SPVFLISRLAEHTRNLSADARASLLLVDPFAPTEGAPRLTLIGRAEPLTLSAAERARC
                     LRLHAAAEQYLQLGDFAFWRLRPERLRVIGGFAAAGWVSGEAYLGAPQLSLDDEQHLL
                     DELAAETGGTVLGVDAYGADGVDAAGRRWRDEFTAPCTSAEAVRSAWATRRRCGG"
     gene            5269..6447
                     /locus_tag="PKN55_00035"
     CDS             5269..6447
                     /locus_tag="PKN55_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase family protein"
                     /protein_id="HNX69660.1"
                     /translation="MTKDVVVLSAVRSAVGTFNGSLAGLEPSDLAGRVMKEAIARSGV
                     DAQSINYVTVGNCIPTESRFAYVARVASIQAGLPMESVAMAVNRLCSSGLQAVVSTAQ
                     SIMLGDCEYGIGGGVEVMSRGAYMLPALRSGARMGNTPAIDMMVDTLTDPFGVGHMGI
                     TAENLASKWGISRDEQDAFALESQQRAAKAIAEGRFAGQIVPIVLKSRKGETVFDTDE
                     HPRATTLESLAAMKPAFKKDGTVTAGNASGLNDGAAFLVLADAAAAANSGYKPMARLV
                     SYAVAGVPNDVMGEGPIPATKLALKKAGLSLDQIDVIESNEAFASQALAVAKGLELDL
                     SKTNVNGGAIALGHPVGCSGALIATKALHELHRVQGRYALVTMCIGGGQGIAVVFERL
                     "
     gene            complement(6616..7134)
                     /locus_tag="PKN55_00040"
     CDS             complement(6616..7134)
                     /locus_tag="PKN55_00040"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNX69661.1"
                     /translation="MSDPLSLPTLPAALDAQGVRLRARQSGDQLAVDQLYQQWRIEQF
                     VVGGLPLADCQQLASQQCRAQRQHYRLLEGPLAWGVVEQHGQCVGRLYLQQHGDDLHI
                     LDILLQPASRNHGLGSALLTACHAHAAALGCTQLSLLVELNNPRALLLYQRLGFVLTG
                     VNGVHGQMVWPV"
     gene            complement(7192..8808)
                     /locus_tag="PKN55_00045"
     CDS             complement(7192..8808)
                     /locus_tag="PKN55_00045"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNX69662.1"
                     /translation="MKNLSVRTQLLTLVVLAVVGFLTLGGMVLRSEYQALRAASIGKL
                     TWLTESTLRIITHYEDAASQGKLSETEAQARAKAAVMAARYGKDGYFFIFDAQMNYVG
                     HPIKPALDGTSVYKLKDAKGRNLGELFAQALANGSGLAAYDWVKPGDSEESEKISKVL
                     ASPRWKWAVGTGQHIDDVNREILQSAISLLVIVGFGTLTLVVIGLLIARNIVRQLGGE
                     PAYAAQVVASIAAGNLNTPVQHDNQGLLAAIAGMQQQLRTMVSQVASQANRLMDMSQG
                     LSGHVGQLSQSASQQSESASSMAASVEEMTVSINEIASHAGQAREVSQQAGVQSRESG
                     RVVRRAAEEMAKIHDSVEAASHTLTTLVDDVSGISSIVGVIRDVADQTNLLALNAAIE
                     AARAGEQGRGFAVVADEVRKLAERTSQATGEITDKISRIQQLSGQSRQSMDAAVEQMG
                     AGVALAREGGTAIAVIEGRTDEVVSTVNDISLALKEQSLASNDIALRIEHIAQSASDN
                     ATAVDAAAQASHDMHAVAASLQTSVRGFQT"
     gene            complement(9392..10651)
                     /locus_tag="PKN55_00050"
     CDS             complement(9392..10651)
                     /locus_tag="PKN55_00050"
                     /inference="COORDINATES: protein motif:HMM:NF010675.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HNX69663.1"
                     /translation="MRCKNAFYAQSGGVTAVINASAAGVIETARQHPTRIGTVYAGRN
                     GILGALDEDLIDTRHESAADIAALRHTPGGAFGSCRYKLQDLQQHRAEYQRLIEVFKA
                     HDIGYFFYNGGGDSADTCLKVAQLSAALGYPIQAIHIPKTVDNDLPITDCCPGFGSVA
                     KYVATSIREAGYDVASMAKTSTKVFILEVMGRHAGWITAACGLASEAEGEPPHLLLFP
                     EIPFEPKLFLDQVDACVQRHGYCVVAVSEGIRQADGRFIAESGLTDAFGHAQLGGVAP
                     VLANLVKSALGYKYHWAVADYLQRAARHLASAVDVEQAWALGARAVELALAGHGGVMP
                     TIVRLSDVPYQWTLGMAALEDVANVEKFMPREFISVEGFHITDACRRYLAPLIQGEAW
                     PPFVNGLPHYVRLQNRAVPRQLPPFSH"
     gene            complement(10749..11405)
                     /gene="adk"
                     /locus_tag="PKN55_00055"
     CDS             complement(10749..11405)
                     /gene="adk"
                     /locus_tag="PKN55_00055"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018749130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004017 - adenylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HNX69664.1"
                     /translation="MKLILLGAPGAGKGTQANFIKDKFGIPQISTGDMLRAAVKAGTP
                     LGLEAKKIMDAGGLVSDEIIIGLVKDRIAQPDCANGFLFDGFPRTLPQADAMQAAGVT
                     IDYVVEIDVPDSEIVERMSGRRVHVASGRTYHVKFNPPKVEGVDDETGEPLIQRDDDS
                     EATVLKRLAVYHEQTEVLVGYYSKMAASGDASAPKYVKVPGVGSVDGIRDAVFRALGA
                     "
     gene            complement(11448..12206)
                     /gene="kdsB"
                     /locus_tag="PKN55_00060"
     CDS             complement(11448..12206)
                     /gene="kdsB"
                     /locus_tag="PKN55_00060"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011136891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HNX69665.1"
                     /translation="MAEFRVVIPARLHSTRLPDKPLADIAGLPMVVRVAQQAAKSGAA
                     EVVVATDHARILQACAEHGVAARLTSADHPSGTDRLAEMADLLALADDDIVVNVQGDE
                     PLIDPALIDRLAALMAASEAPMATLAHPIHDAADMFNPNIVKVTLDRRGHALYFSRAP
                     IPYARDAFAHRTDALPPELPVLRHIGMYAYRAGFLRHYRELAPSALEAIEALEQLRVL
                     WHGHAIAVGVVEQAPPGGVDTPEDLARVRALFHP"
     gene            complement(12199..12387)
                     /locus_tag="PKN55_00065"
     CDS             complement(12199..12387)
                     /locus_tag="PKN55_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011136892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HNX69666.1"
                     /translation="MDSKLLEILVCPVCKGPLVFDKSAQELICKADRLAFPVRDGIPM
                     MLESEARELPADEEVRGG"
     gene            complement(12368..13408)
                     /gene="lpxK"
                     /locus_tag="PKN55_00070"
     CDS             complement(12368..13408)
                     /gene="lpxK"
                     /locus_tag="PKN55_00070"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008954898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HNX69667.1"
                     /translation="MPDSPSTFAERLQAFWHGRRDPWLALWTLPLSWLFAALAGLRRA
                     AFACGLLRRERLPVPVVVVGNIHVGGAGKTPLTLALLAELRAAGLRPGVISRGYGGAA
                     RQAQAVHARDDPAQVGDEPLLYAQAGFAVAIGRRRSEAGRALLAAHPEVNVILADDGL
                     QHYALARDIELAVVDGGRGFGTGRLLPAGPLREPVSRLARVDAVVVNGDAAPPDLPAH
                     PRCFHMRLQPGALYQLSQPARRCAPADLPGRVVALAGIGHPQRFFDTLRQQGLTPARC
                     LAFADHHAFTAADLPADADAVVITSKDAVKWQALDNGKLWVLPVTAQLDAGLVSWLVS
                     TLKELRHGQQAA"
     gene            complement(13515..14903)
                     /locus_tag="PKN55_00075"
     CDS             complement(13515..14903)
                     /locus_tag="PKN55_00075"
                     /inference="COORDINATES: protein motif:HMM:TIGR00341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00341 family protein"
                     /protein_id="HNX69668.1"
                     /translation="MDDESQKKNLSLAIRVKHIMRERFSLHDDKAEDTEIEARIRDGV
                     ELRGATPWVLIFAIFVASVGLNVNSTAVIIGAMLISPLMGPIMGAGLGVAVYDFDLVK
                     RSLTNLGIATFISLVVSALYFSLTPLQQTQSELLARTSPTIWDVLIALFGGLAGIVGI
                     TRQEKSNVIPGVAIATALMPPVCTAGFGIATGQWQFAGGALYLYAINCVFISLATVVG
                     IRALRLGRHGFANQKTEKRVKATLLALALITSVPSGYLALDLVRQEVFRSKAQEFVDR
                     EFTFDKSQLVDTKIDPDKRVIDLSILGEPISPEALHHIEAKLATANLEGTKVVLHQAG
                     ENRIDLTALKSSLLSDLLRESQDTVKRRDTQVQNLKAELAARDAILGQANGISKELRQ
                     LYPEVTRVLLADGVSVTSEESKEKVVYITITTRIAMSKEIHQRTEDWLKVRLNLNSIV
                     LNFDTEGQPLKR"
     gene            complement(14935..15783)
                     /locus_tag="PKN55_00080"
     CDS             complement(14935..15783)
                     /locus_tag="PKN55_00080"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HNX69669.1"
                     /translation="MNTSYGHSFISSRALRLAFSLVGTSLIVGLGLLRVHTQAQFTFA
                     SLMLLPVLANTWLVGRVGGWLSAILAALMWGYADHVSTGAHETTWIPGLNTFIHLINY
                     GVVVELVHKVQELLQREANNARVDRLTGLLNRRGFMETFEEQIKLAHRLNASFSIAFI
                     DLDRFKQLNDTRGHKEGDAALKMAGTILLNATRSTDVIGRLGGDEFCVVAFSRSPNDA
                     EIEALRLHAQLTEALSKFTPVGASIGVAFFDEAELSATEMIQKADEIMYSVKKAGKGS
                     VLVKVV"
     gene            complement(15780..16676)
                     /locus_tag="PKN55_00085"
     CDS             complement(15780..16676)
                     /locus_tag="PKN55_00085"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HNX69670.1"
                     /translation="MMPIVQVEKRQLIDQFISRLQSVFSVDLLADLLSRLILALVVFS
                     VVMLTWIIINRFVSHRLKRRVDSTTAAMLQTLTKTAFISLGLLAGLNAAGVQTGTLLA
                     SAGVLSLTVGFALKDTLSNIVSGFLIFMDRPFTIDDLVEIDGQYGRVDRITLRTTRIV
                     TKDGRMLAVPNASVMNKTIASYTNFPHQRIDVRVTIAVTEDIERVRSILLDLVRHDPS
                     YLKEPAPRMVVVEINDYNLAIELQAWLDDERQHIEKRFALREAAFKALVAANIEMPLE
                     TIQLAPHKIEVNKSLLPTESNT"
     gene            complement(16676..17983)
                     /locus_tag="PKN55_00090"
     CDS             complement(16676..17983)
                     /locus_tag="PKN55_00090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019922768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative Na+/H+ antiporter"
                     /protein_id="HNX69671.1"
                     /translation="MVLLVASSPVTLQRNSVTITATQWTGAALFGIAIIHTFATKLFE
                     RLAHYRPTHAGLWHLLGEVEVVFGFWAMVLVVAMFFIDGADTAIAYVKSRNFTEPVFV
                     FAIMVIAATRPILQAATGAIRALGSSMPLPGAMGFYFVVLATVPLLGSFITEPAAMTM
                     AALILSRRVFSQSASMRMKYATLGVLFVNISIGGTLTPFAAPPVLMVAGTWQWDTGFM
                     LANFGWKAAIAVLINSIGATMIFCKELAQIEVSNEKKNGHTTPWPLTIAHVIFLMAVV
                     IFSHHPKFILGLFLFFLGLSHAYEKYQDRLILREALLVSFFLAGLVVLGGQQRWWLQP
                     VLTSMTNEQVFYGAVALTAFTDNAALTYLGALVPGLSDEFKYALVAGAVTGGGLTIIA
                     NAPNPAGIAILRNHFDDNTVNPMGLLAAAILPTLVAVFAFKFL"
     gene            complement(18177..18590)
                     /locus_tag="PKN55_00095"
     CDS             complement(18177..18590)
                     /locus_tag="PKN55_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014088232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HNX69672.1"
                     /translation="MNFSRGRKREEPEINFIPLIDLLLVILIFLMVTTTYNRFAELKI
                     NLPTASADAPQEKPLEIRVAVNPAGEYLVNQQPVAANALGDALKAAAGGQADPSVVIQ
                     ADGKAPHQAVVTVMEAARQASLSKLTFATQAPAAQ"
     assembly_gap    18637..18736
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(18756..19361)
                     /locus_tag="PKN55_00100"
     CDS             complement(18756..19361)
                     /locus_tag="PKN55_00100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018150713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HNX69673.1"
                     /translation="MLSIIEAAGWPIWTIILASVVSLAIIFERFYSLRQSLVAPAGLL
                     EDVLNRSQAGDTRALYEQLANGAPLARILAAGLKNQRASREVMKEAIEEAGRVVAHEL
                     ERFLTTLGTIAAMAPLLGLLGTVVGMIELFGASSPTGTNPQQLAHGISVALYNTAFGL
                     VVAIPSMIFYRHFRAKVDALLVSMEQQAIHLVDVVHGDRDR"
     gene            19740..21089
                     /gene="xseA"
                     /locus_tag="PKN55_00105"
     CDS             19740..21089
                     /gene="xseA"
                     /locus_tag="PKN55_00105"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008954895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HNX69674.1"
                     /translation="MQDVLPELVSVSELNRLARGLLERGLPPLWVSGEISNLTVAASG
                     HAYFSLKDKGGQIRAVMFRGRVAGLPFRLREGVQVEAFGQVSLYEARGDYQIQLDRMR
                     EAGLGRLFEAFERLKRQLGEEGLFDPARKRPLPAFPRRIGIVSSPSGAALLDVVSTLR
                     RRMPSAHLVLYPTLVQGEAAPAQIVAAITQAALRQEVDVLMVCRGGGSIEDLWAFNDE
                     TVARALAACPIPTISGVGHETDFTIADFVADLRAPTPTGAAEQVCPDRQALLAELAQR
                     EQRLRRAMQRLLHSRMQALDALSARLTHPGQRLRQQAQLLQRDQARLASLARSAVQQA
                     GWRLERLAERHARLRPQAAAAQAGLDALAERLRQAMRRQLAQAALHQQHLADRLSALN
                     PQAVLQRGYAIVENRKGQAVRSPDALKQSERVTLRLADGVTQAEIRHPDGGQRQLDL"
     gene            21094..21405
                     /locus_tag="PKN55_00110"
     CDS             21094..21405
                     /locus_tag="PKN55_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011137936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HNX69675.1"
                     /translation="MAQPWFLYVLACQGGGLYTGVTPDVARRWAAHLAGKGARYTRSF
                     PPQQLALVVEFASKGEALSAERAVKAMRAADKRAWLLSCGGQLPAPSISADTSVRPLA
                     A"
     gene            21569..22339
                     /locus_tag="PKN55_00115"
     CDS             21569..22339
                     /locus_tag="PKN55_00115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014086652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HNX69676.1"
                     /translation="MEWGFVVAGLVVGFVVGLTGVGGGSLMTPILLWFGINPATAVGT
                     DLLYACITKMGGVWVHHQHKQIDWRITGWLALGSMPAALLTVLVLHGLHLETHYIERL
                     IKLMLGVALMLTAIAILFKPWILRQLARMVPPRPEGVLPVKATVLTGVMLGVIVTLSS
                     IGAGALGTLAIFALYPLLPIARLVGTEIAHAVPLTLVAGLGHASMGHMDYGLLAQLLM
                     GSLPGIWLGSHMTRRVAEQWLRPALAVMMAMIGAKLVF"
     gene            complement(22422..25112)
                     /gene="glnE"
                     /locus_tag="PKN55_00120"
     CDS             complement(22422..25112)
                     /gene="glnE"
                     /locus_tag="PKN55_00120"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012697614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HNX69677.1"
                     /translation="MSLSPVHALSAAQAHSHYLTRLFAADPALAERIAVEAAEPFSLR
                     HMQEGFASLPADSEEALKRNLRKLRQRMMAHLITRDIGRLADLNEVVSTISQFAEFCV
                     TTALAHVQPLLAHFGQPIGEDSGQPQSLIVVGMGKLGGGELNVSSDIDLIFIYPEDGQ
                     TEGGTRHISNHEYFTLLGKKLIAALSEPTGDGFVFRVDMRLRPYGDSGPLVMSFAALE
                     NYLLSQGREWERYAWIKGKALCGDAEGLNALIRPFVYRKYLDYNAYGAMRELYAQIQR
                     EVARRELTDNVKLGAGGIREVEFIAQVFQLIRGGREKSLQVRSTREAMARLAELKLLE
                     PETVSELLEAYTFLRDVEHRLQYLDDQQTQTLPAGVEARQRVAGSMGFADWDGFVSAL
                     NRQRQRVNRHFQQVFLLPTEQHGGHPLASLWLDVGEAPEAVVERLASQGFQDAPAVAH
                     QLSTLARSQRYLQLPAATRKRFDALMPPLIEVSAQCGNPDVTLARIINLMEAISRRAP
                     YLALLAEYPQTLRRLVTLYSSSPWVSSYLTRHPILLDELLDARELYAEPDWPALAAQL
                     EQQMAECGSDVEAKMDTLRHVQHAQQFRLVAQDLAGMWPLEALSDQLSQLADMILDAT
                     LRHAWQDVAKRHADTPRFAIIGYGKLGGKELGYVSDLDVIFLYEDEHPDAADGYARLA
                     RKLSAWITSATPAGILYDLDLRLRPNGQSGLLVSTVEAFRQYQARDAWLWEHQALTRA
                     RFVAGDAAIGDAFEKVRFEVLSQPRDLAALRQEVLTMRARMLETHPAQPDKLKHARGG
                     IIDLEFIVQYLVLAYSHYHPELTGNIGNIALLHLAANAGLIEHDQAEACQAAYRHYRR
                     LQHAQRLNEHNSVEVDDVLRRHYADVQALWEAVFGQPDVV"
     gene            25202..29044
                     /locus_tag="PKN55_00125"
     CDS             25202..29044
                     /locus_tag="PKN55_00125"
                     /inference="COORDINATES: protein motif:HMM:TIGR02099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdP family protein"
                     /protein_id="HNX69678.1"
                     /translation="MNVFPRLISARLLAWRQRPAVRRALRAALWLIVSAALLLAALAC
                     AIQFWLLPRLGDYKPALAQALSQATGQRVDIDELGGGWRHGHLVLFVRGVSVSQPQSG
                     ATQRFAKLELAPSLSSLWRLEPHFSRIALSGPVIHIRRDARGAILINDIDLTQGSGDT
                     ALLDWLLRQSEVSIDQATLHWRDEFAGLAELTLTDGRVVLRRGLFSHSLAIGARPPSD
                     WLSSFALAMDWRGDRLADWKTWRGRVRLDVGGVRLSAWENYLNFNSSRVLPQGEGQSS
                     VELNFDGPRIEALNAQLSLRNVLLRLNPSEAAVRVPVLSGKLALAQTRDGVWTLAAQD
                     LLAVAENAAIFNHADIQGEWKDGANGHGKLTLSQVDLAAIKPLLRHLPLEQNAAWKAL
                     APTGFVEKVQVGWRGDIVAPSRYQVSGAFRQLGWQGSGVLPSVTGMSGELNFDESRGE
                     LNLRNQGAASVTLPGVFIAPLQFADLNARVNWTREAQGVAVDLGKIRFANADVSGELS
                     GRYRYVPGHAGLVDIQATLGRAPANRVPAYLPLSIDHDTRNWLTQALRRGHADGAQLL
                     LRGDLDHFPFADASKGNFEVHTRADGVTLAYAPGWPALENISGELRFIRNRMEIANAS
                     GQVMGARLFNVNGVLPDIEHSSSHLQIEGQASGPTQAFLQYLRATPIDRVLGGLTRAA
                     QTQGAGALKLKLDIPLAHADATQVSGEYQFLRNGVRLVGYAVPPLSEVSGVLRFSETG
                     ADSDGLRFTALGGAGQLRIAREPQGRMRFGLNGHADMRAAARQYLPWLAPHLNGQSEY
                     RGEFHISRDLDALWLESSLRGVSSQLPMPLAKQAADTLPLRLDLAPEARGGLRLGVKV
                     GQTLQGQFALADSGEIQRGALRLGAHASLPAMPARGGVAVAVQDERFELDPWLSLGSG
                     DADGAWPTLSVALDTRQLLAQGRVLHQVKAQLQSSHGDWEGQVSAQELAGQLHWQAGA
                     HKLSAKLSRLNLPLSSSANEPNDTSLGRQAANLPALDITVDALSYQQQPLGHLQLAMQ
                     PRSNGWQLDQLWWSGPEGRARLTGMWQRDGREGVTALRGQFDSQDLGGLLARFGYPDT
                     LRRGRLTAQGALQWQGGVLSPVLSSLRGDLSLRAENGQFAKINPGVGRLLGVISLQSL
                     PRRFQLDFHDIFSEGFAFNALTGKVNVDNGVFTLNDMKMTGPAADVSLRGHADLGRDR
                     QQLRVTVEPHLSEGVALAGAALLNPVLGVAALAAQKVLQDPVGKLFAYDYEITGSLAA
                     PDIRKLDRFGGDAPREKP"
     gene            29048..29857
                     /locus_tag="PKN55_00130"
     CDS             29048..29857
                     /locus_tag="PKN55_00130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018150906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HNX69679.1"
                     /translation="MTASTLTVASIQMVSAPDVPSNLATAARLVAQAADAGAQLVVLP
                     EYFALMGLNDTDKVKAREPWGDGPIQRALADMARRHQLWLVGGTLPLEAPEPDKVYNS
                     CVLYGPDGQAAARYDKIHLFGFSGLGERYCEADTITPGKEIVAVDTPLGRMGLSVCYD
                     LRFPELFRAMGEPDIIILPAAFTAITGQAHWEPLLRARAIENQCFVIASGQGGVHASG
                     RATHGHSMVIDPWGVIVAEQAQGEGVVLAQLDRRQLASVRTRLPALAHRVF"
BASE COUNT         5619 a         9217 c         9399 g         5675 t
ORIGIN      
        1 ccggcggccg agtttgccgc cgcctgccag cgcctgctgg ggccgtggct ggccacgcgg
       61 catgccggct tgcagctgga ttttccggtg gatttgcgct ttccgggttc ggccagcggc
      121 acgctggaca gcaccaatct gcaacccacg gattggctgc gcatggcgcg ctgggtgctt
      181 gcccattaca ccgagtacga cgcctttgtg gtgttgcacg gcaccgacag catggcgcac
      241 agcggcgcag ccttgtcttt tttgctgaat ggctttgccg ccgatggcca gctgagcgcg
      301 gcgctgtcgc ggccggtgct gctgaccggc tcgcaactgc ctttgtttgc gcaaagcgcc
      361 gctggcctga cgctgaacgc gcagggcgac gcgctggcca atctggatgg cgcgctgcac
      421 gcctgccagc gcggtgtgtg cgaggtggcg gtgtattttc agcgggcgct gtggcgcggc
      481 aatcgggtgc tgaaaaccga cgcgcaggcg tttgccgcct ttgccgcgcc caatgatttg
      541 cccttgatcg aagacgggct ggaatgccgg gtgcatggcg agcgtttttt gccgccaccg
      601 gccagcccgg cgcgcagcct ggaatccccg gccgcccgcg ccgcccagct ggcactgctg
      661 gatgccttgc aaacccgctt ggcggcccat ccggtgtgga cgctgctggc gcagccggcc
      721 gacggccagg ccaatccgtt ggcgcaggcc atccacgcgg cggtggccgc tggcgcgcgc
      781 ggcttgctgt tgctgggcta cggcgcggga aacttcccca gcggcgacgc cagcgagccg
      841 gcgcgcggcg cggtgtatca ggcgctggcg caggcgcgcc agcagggcgt gcaactggtg
      901 ggcggcacgc aaacgccgca tggcggggta aacggccaga tctacgccgc gggtgcctgg
      961 ctggcgcagc tgggcgcgct taatcctggc gacatgaccc cggtggctgc acaggtcaaa
     1021 ctgagcgtgt tattggccct ggccgagctg catggctggt cggcggcgca ggtggccatg
     1081 ttgttccagc gcgccttgct gggcgaaatg cgcgacgcca ccttgctgga cagccgtcgg
     1141 ggcggcgtgc tgcgccccgg cgaggctttg ctgagccagg atggcggcgc gagcgtgtct
     1201 ttgtcgctgg atgagggcct gcgcctgtgc gccgccgatg gccgcctgct gtggcaagcg
     1261 cccggcagcg cccaggccag cttgctgcga ctggggctgg atggcgcgtt gagtctgctg
     1321 gggcgcagcg gcgaaatcct atgggccacg ccgccgctgg gcggcgcggc ggcctggctg
     1381 cggctggatg gccagtgcgc cgacggctcg ctgaccctgc gcctgggcga cgccggcgcc
     1441 gacatcggcc gggtgctgtg gtcggcggcg gatggccagg gcgcgctgga aaggtaagca
     1501 tggcgtacgc atgcctggct ctgaacgaga gcagggcact gaacggtaga atgcttgagg
     1561 ggcatctggc ccatgctgat ggagttttct aggtgatttt acggcttttg gacactgtcc
     1621 aatcagcatg cttcgtgcta agctgatgtt gcaatgcaca aaattacacg ggcacgcggc
     1681 gcatggcctg gcgctgcccg gacatcagag ggaatccatc atgcatgccc tgctcgctca
     1741 tgcccgcctg atggcggact acaatcaatg gatcaacacc cggctgtatg cggcggcggc
     1801 agaattgccg catgacatgc tcacccagga tcgcggcgcg tttttccgct cggtgctggg
     1861 cacgctcaac cacgtcatgg tgggggaccg ggtgtggttg tcgcgctttg cccgccaccc
     1921 ggcccgccat gccgcgctgg aatcgtttcg gcgcatgtcc gccccgcagc gggtggatgt
     1981 gatgctgcac gacaccctgc cggagctcac tttggcccgc catcagctgg atcaaaccat
     2041 catcgactgg tgcaccgagc tcaccgccga cgcgctggcg catccgctgg cctaccaaag
     2101 cctgaaaggc gtgccggcgg tgcggccgat gggcagcctg atgatgcatt tttttaatca
     2161 ccagacccac catcgcggcc aagtgaccac cctgctgtcg caatgcggcg tggacatcgg
     2221 cgaaaccgac ctgctgggcc tgattccgca gtgtgacgaa gagccggtat gagcgcgttt
     2281 gccgcccgcg cggcgacgct ggctgatttg cctgtggtgt gcagttgggt ggacgacgcc
     2341 gatacgctgt tttatctgca tccgcgcgcg cagtttccgc tgaccgtggc ccagctggcc
     2401 gaggcctttg ccgcgcgcgc gctcaacacg gtgttgctgg ccgatggcgc gccggtggcg
     2461 tttgccaatt gctaccggca cacgccgggc gagtgcgcga tgataggcaa tgtgattgtg
     2521 gcgccggcct ggcgcggtcg cggcgcagcg cgcgccttgc tggccgccat ggctcagcag
     2581 gccatcgccc gctggcaggt gcgcgagctg ggggtgtcgt gctttaacgg caatgtcgcc
     2641 ggcctgctgt gttatccggc gctgggcttt gtcccgcacg cggtggaagc gcgccagcgc
     2701 gatggccagc cggtggcgct gctgcatttt cgggcgtcag cggcggcctt ggccgccacg
     2761 gtggaaccct gttcaccgcc ggcagtctga accgctttat ccttgccgga gcgtgatccc
     2821 atgcgttttt ctgtgcttgc ccccttggcg ctggccagcg ttttgttcag cgcaggcgcg
     2881 ctggccgagc cgctgaacta caaccagatt cagctgggcg ccgacgcccg ccgcgaaatc
     2941 aataacgaca ccctcaatgc cgtgctgttc accgaagaca gccacgccag cccggcacgg
     3001 ctgagcgccg tgctcaacga gcgcatggcc gccgcgctga aaatcgccag ccagcatggc
     3061 aaggtcaagg ccagcagcgg ccagctgtcc acctggccgg tgtacgacaa taaccagcgc
     3121 gtcaccggct ggcgcggccg cgccgagctg cgcctggaaa gccgtgacat tgaacaagcc
     3181 tcgcagctga ttggccagtt gcaaaaccag ctgcaaattg cccaggtggg ctttagcgtg
     3241 tcggacgagg cgcggcgcaa ggtggagtcc gagctgatcc cgcaggcgat tggcgcgttt
     3301 cgcgacaagg cgcggctgat tgtgcaggcg ctgggcgggc gcgactaccg cctggtggaa
     3361 atgcgcgtgg atcaggccgg ccagccgggc ttgcaggtgt ttcgcgccca gcgcgcgatg
     3421 aaggccggcg cgccgatgat ggccgccgaa gccgcgccca gctttgcgcc gggggtgagc
     3481 gaaatcagcg tgcaggtgca aggcacggtg caaaccgcgc cgtaaatccg ggtgaggcgg
     3541 cgctgacaca gaaaaccgtc aatggccgcc cacagcatgc ggcgagtgtg gcaagatact
     3601 gcacaccctc catcccgaaa tactgtttgc agccccatga aaattctcag ttcattgacc
     3661 agcccttacg gccgcaaggc acgcattgtc ctgctggaga aaaaaatcga ctgccagctg
     3721 gacgtggaca tcccctggga tgaagacagc caggtgcctg aatacaatcc gctgggcaag
     3781 gtgccggtgc tggtgctgga tgacggcacc gcgctgtacg attcgcgggt gattgtcgaa
     3841 tacctcgaca agctctcgcc ggtgtcgcgc ctgctgccgg cagaaagccg cccgctggcg
     3901 atgaccaagc gctgggaagc gctggccgat ggcgtcatcg acgccgccgt ggccatctac
     3961 ctggaaaaac aacgcgaatc cacccagcag aaccccggct ggatcgagcg ccagtttggc
     4021 aaaatgcgcc gtggcctggc tgccatgtca gcagatctgg gcgaaaaaac ctggtgcaat
     4081 ggcgaatcct acagcctggc tgatatcgcg gttggttgca gcctgaacta cctggatctg
     4141 cgctttgccc atatgaattg gcgtgagcaa tatcccaatc tggctgcgct aatggccaag
     4201 ctggaagcac gtcagtcttt catcgacacc cgcccgccag tgctgtaacc ccttcccgcc
     4261 cgccgcccct tcggggacga cgggcggctt gctttggccc cttggcggcc cccccaagat
     4321 cacagcggct cacaaaacgc tcacaaaatc ccatgctgcg atttcagcag ggtttggtga
     4381 gtcgcgctgg cgagcttagc cgccacagcg ccgccgcgtc gcccacgcgg agcgcaccgc
     4441 ctcggcactg gtgcaggggg cggtgaattc atcacgccag cgccggccgg cggcgtccac
     4501 cccatcagcg ccataggcgt ccacgcctaa tacggtgccc cctgtctcgg cggccagctc
     4561 gtccaacaag tgctgttcgt catccaggct aagctgaggc gcgcccagat acgcctcgcc
     4621 gctcacccag ccggcggcgg caaagccgcc aatcacccgc agccgctcgg ggcggaggcg
     4681 ccagaaggca aaatcgccca gttgcagata ctgttccgcc gccgcatgca ggcgcaggca
     4741 gcgcgcgcgt tctgccgcgc tgagcgtcag cggctcggcg cggccgatca gcgtcagccg
     4801 tggcgcaccc tcggtcgggg cgaaagggtc caccagcaac aggctggcgc gggcgtcggc
     4861 gctcaggttg cgggtgtgct cagccagccg gctgatcaag aacaccgggc tgccgtcggc
     4921 cagcgcgacg agattgacgg cggacgcgaa cggccagccg tccagctcgg ccgacagggt
     4981 ggccagcgcg ccctgtgacg cgcggcgcaa cagccgttgc gccaaatgat agggttcttt
     5041 catgcaatct ccatgcgcga acaaattcag ggtaaaaaga ccgggatggg caggccattt
     5101 ggccggatga tggtgtgaca ctttgcctgt ggcgcaagtg atggtaaagt gcccgtcggc
     5161 ccgatgctgc ggtgcagcat aattcatgtg cgcatcccgc gtcggcccca tcgcaggcat
     5221 gtcgccgcgc cctgccggcg gccatgaaaa aatagaggaa tagatacaat gacaaaagat
     5281 gttgtggtgt tgagcgccgt gcgctcggct gtgggcacgt tcaatggttc gctggctggc
     5341 ctggagccgt ccgatctggc gggccgcgtg atgaaagaag ccatcgcccg ttctggcgtg
     5401 gacgcccagt cgatcaacta cgtgaccgtg ggcaactgca tccccaccga gtcgcgcttt
     5461 gcttacgtgg cccgcgtggc ctcgatccag gccggcctgc cgatggaatc ggtggcgatg
     5521 gcggtgaacc gtctgtgctc gtccggcctg caagccgtgg tcagcaccgc gcaaagcatc
     5581 atgctgggcg attgcgaata cggcattggc ggcggcgtgg aagtgatgtc gcgcggcgcg
     5641 tacatgctgc cggcgctgcg cagcggcgcg cgcatgggca acaccccggc catcgacatg
     5701 atggtggaca ccctgactga cccgtttggc gtgggccata tgggcatcac cgcggaaaac
     5761 ctggccagca agtggggcat cagccgcgac gagcaagacg cctttgcgct ggaatcgcaa
     5821 cagcgcgccg ccaaggccat cgccgaaggc cgctttgccg gccagatcgt gcccatcgtg
     5881 ctgaagagcc gcaagggcga aaccgtgttc gacaccgacg aacacccgcg cgccaccacg
     5941 ctggagtcgc tggccgccat gaagccggcg ttcaagaaag acggcaccgt caccgccggc
     6001 aacgcctcgg gcctgaacga tggcgccgcc ttcctggtgc tggccgacgc cgctgccgcc
     6061 gccaattccg gctacaagcc gatggcgcgc ctggtgtcgt acgccgtggc cggtgtgccc
     6121 aatgatgtga tgggcgaagg cccgatcccg gccaccaagc tggcgctgaa aaaagccggc
     6181 ctgagcctgg accagatcga cgtgatcgaa tccaacgagg cgtttgcctc gcaggcgctg
     6241 gccgtggcca agggcctgga gctggatctg agcaagacca acgtcaacgg cggcgccatt
     6301 gccctgggcc acccggtggg ctgctcgggt gcgctgatcg ccaccaaggc gctgcacgag
     6361 ctgcaccgcg tgcaaggccg ctacgcgctg gtgaccatgt gcatcggcgg cggccagggc
     6421 attgccgtgg tgtttgaacg cctgtaatcc cccgctgcgc tgaaaatcac gcgcccccgt
     6481 ccggtgatgc cggcgggggc gttgtgcatg aggcgccgag aaacgcgaac acaatgcagc
     6541 gaagcgatcc tggctgggcg tgtgcagaca cggcaaggtc acgccacaac gccaggcgca
     6601 ttgtgtgagc gctttttaca ccggccacac catctggccg tgcacaccgt ttaccccggt
     6661 cagcacaaaa cccaggcgtt gatacagcag cagggcgcgc gggttgttca gttccaccaa
     6721 caggctgagc tgcgtgcaac ccagcgcggc ggcgtgcgcg tggcaggcgg tcagcagcgc
     6781 gctgcccagg ccgtggttgc ggctggccgg ttgcagcagg atatccagga tgtgcaggtc
     6841 gtcaccatgt tgttgcaggt ataagcgacc gacacactgc ccatgctgtt ccaccactcc
     6901 ccaggccagt gggccctcca gcagccggta atgctggcgc tgtgcgcggc attgctggct
     6961 ggccagttgc tggcaatccg ccagcggcag gccgccgacg acgaattgtt cgatgcgcca
     7021 ttgctgatat agctggtcta ccgccagttg gtcgcccgac tgacgggcac gcaaacgcac
     7081 accctgcgcg tccagggcgg cgggcagggt gggcaagctc aggggatcac tcatctcaag
     7141 gcatcctggc gccaccctgc ggtggcgccg tacaggccat caggccggga cttatgtctg
     7201 aaaaccgcgc accgaggttt gcaggctggc ggccacggca tgcatgtcgt ggctggcctg
     7261 ggcggcggcg tccaccgcgg tggcgttgtc gctggcactc tgggcaatgt gttcgatgcg
     7321 cagggcaatg tcgttgctgg ccaggctttg ctctttgagc gccagcgaaa tatcgttgac
     7381 cgtgctgacc acttcgtcag tgcgtccttc gatcacggca atggccgtac caccttcgcg
     7441 cgccaatgcc acgccggcgc ccatctgctc gacagcggca tccatgctct ggcgcgactg
     7501 gccagacaat tgctggatgc ggctgatctt gtcggtgatt tcgccagtgg cctggctggt
     7561 gcgttcggcc agcttgcgta cttcatcggc caccacggca aaaccacgcc cctgttcgcc
     7621 ggcgcgggca gcttcaatgg cagcattcag cgccaagaga ttggtctggt cggccacatc
     7681 gcggatcacg ccaacaatcg agctgatgcc agatacatcg tccaccagtg tggtcagggt
     7741 atgcgaagcg gcttcgaccg aatcatgaat cttggccatt tcttctgccg cgcggcgtac
     7801 cacacggccg ctttcgcgcg actgcacacc ggcctgctgg ctgacttccc gcgcctgacc
     7861 ggcgtggctg gcgatttcgt tgatgctgac ggtcatttct tctaccgatg ccgccattga
     7921 cgaagcggat tcactttgct ggctggccga ttgcgacagc tggcccacat ggccggacag
     7981 cccttgtgac atgtccatca gccggttggc ctggctggcc acctgactga ccatggtgcg
     8041 caattgctgc tgcatgccgg cgatggctgc gagcaagccc tgattgtcgt gctgaaccgg
     8101 agtattgagg ttgccggcgg caatgctggc aaccacttga gccgcatagg cgggctcgcc
     8161 acccaactgg cggacaatat tgcgggcaat cagcaaaccg ataaccacca gtgtcagggt
     8221 gccaaaaccg acgatgacca gcaggctgat ggccgactgc aggatttccc gattgacgtc
     8281 gtcgatatgc tgaccagtgc ccactgccca tttccagcgc gggctggcca gcaccttgga
     8341 gattttttcg ctttcttcgc tgtcgcccgg cttgacccag tcatacgccg ccaggccgct
     8401 gccattggcc agtgcctgag caaacagctc gcccagattg cgccccttgg catctttgag
     8461 cttgtagacg ctggtgccgt ccaaggcggg cttgatcggg tggccaacgt agttcatctg
     8521 ggcgtcaaag atgaaaaaat agccatcctt gccatagcgc gccgccatca cggcggcttt
     8581 ggcgcgtgcc tgcgcctccg tctcgctgag cttgccctga ctggcagcgt cttcgtagtg
     8641 ggtgatgatg cgcaaggtgc tttcggtcag ccaggtcagc ttgccgatac tggcggcgcg
     8701 cagcgcctga tattccgagc gcagcaccat gccacccaag gtcagaaagc cgaccaccgc
     8761 cagaacaacc agggtcagca actgtgtgcg aacagacaga tttttcatgg atgcgtgagt
     8821 tcctgctgcg catgcgccgt gccgatgcgg cctggccgag catgctttat aagtaataaa
     8881 caaaagttca acagtatacg ccgatgcctg acggaccgtg agcagaaatc ctgcgatatt
     8941 cccctgccag caaaaggtgt atttcccgcc tgggcgagca ccgtctgtca ggaaacctcc
     9001 ccgtatgccg agaaacccaa aggtcgatca aaacatgaca attgaaattg tactttagcc
     9061 aagttgatga atgaacgcag gtttttttgt tgtggcagcg atggggtgcc ctgcacgatg
     9121 cggtgggatt ttttaagagt atcacgagcc aagcttaaat taagctgaat gacacaagct
     9181 atgccaggca aagatttttg gtagatttac ttaattgaca tcaaatgtga agcaattgtt
     9241 tctgcctcaa catatagcaa aataatatgt cagcctggga tattgtgggg gacatgcaac
     9301 agtggcaaat cgacatggcc ctgtgcactg cgacaacccg gcctgcatcc tgccgttccg
     9361 gccagatggc aggctgggtg acggcctgct ttcaatgaga aaacggcggc aactggcggg
     9421 ggactgcgcg attttgcagg cgcacatagt gtggcaagcc attgacaaac ggaggccagg
     9481 cctcgccctg aatcagcggc gccaggtagc gtcggcaagc gtcagtgatg tgaaatcctt
     9541 cgacactgat gaattctctt ggcatgaatt tttccacatt ggccacgtct tccagtgccg
     9601 ccatccccag cgtccattgg taaggcacat cgctgagacg cacgatggtc ggcatcaccc
     9661 cgccatggcc ggccagcgcc aattccacgg ctcgcgcgcc cagtgcccag gcctgctcga
     9721 catccactgc cgaagccaga tggcgggccg cgcgttgcag ataatcggcc acggcccaat
     9781 ggtatttata acccaatgcg ctcttgacca gattggccag cactggcgcc acgccgccca
     9841 gctgggcatg gccaaaggca tcggtcagcc cgctttcggc aataaagcgg ccatcggcct
     9901 ggcgaatgcc ttctgacaca gccaccacgc aatagccgtg gcgctgtacg caggcatcca
     9961 cttgatccag aaacagcttg ggctcaaacg gaatttccgg aaacagcaac aaatgcggcg
    10021 gctcgccttc ggcttcactg gccaggccac aggcggcggt gatccagcca gcatggcggc
    10081 ccatgacttc caggatgaac accttggtgg aggtcttggc catggacgcc acatcgtagc
    10141 ccgcctcacg aattgaagtg gctacgtatt tggcgactga gccaaagccc ggacagcaat
    10201 cggtgatggg caggtcgttg tctacggtct tggggatatg gatggcctgg atcgggtagc
    10261 ccagtgccgc agacagttgg gccactttca ggcaggtatc ggcagaatca cccccgccat
    10321 tataaaaaaa gtagccaatg tcatgcgcct tgaacacttc gatcagacgc tgatattccg
    10381 cccgatgctg ttgcagatct tgcagcttgt agcgacagga gccaaacgcc ccgccggggg
    10441 tgtggcgcag ggcagcgatg tcagcagcgg actcgtggcg ggtgtcaatc aggtcttcat
    10501 ccagcgcgcc cagaatgccg ttgcgcccgg cgtataccgt tccgatgcgc gtagggtgct
    10561 gacgggcggt ttcgatcacc ccggctgccg aggcattgat cacggcggtg acgccaccgg
    10621 attgggcata aaaagcgttc ttgcagcgca tggcacgctc ccgagcaggt gtgatggcgc
    10681 cagtgtaaca aaaacgcccc ggcgggccgt agccgtccgg ggcgttttac ctgggcaggg
    10741 atcgaggatc aggcgcccag tgcgcggaac acggcgtcgc gaatgccgtc caccgagccc
    10801 acgcccggca ccttgacgta tttcggcgcg ctggcgtcgc cgctggcggc catcttcgag
    10861 tagtagccca ccagcacttc ggtttgctcg tggtacacgg ccaggcgctt gagcacggtg
    10921 gcttcgctgt cgtcgtcgcg ctggatcagc ggctcgccgg tttcatcgtc cacgccttcc
    10981 accttgggcg ggttgaactt gacgtggtag gtgcggccag aggccacgtg cacgcggcgg
    11041 ccggacatgc gctcgacgat ttcgctgtcc ggcacgtcga tttccaccac gtagtcgatg
    11101 gtcacgccag ccgcctgcat ggcgtcggct tgcggcaggg tgcgcggaaa gccgtcaaac
    11161 agaaagccgt tggcgcagtc gggctgggcg atgcggtctt tgaccaggcc gatgatgatt
    11221 tcatccgaca ccaggccgcc ggcatccatg atttttttgg cttccaggcc cagcggggtg
    11281 ccggccttca cggcggcgcg cagcatgtcg ccggtggaga tttgcggaat gccgaattta
    11341 tctttgatga agttggcctg ggtgcccttg ccggcgcccg gtgcgcccaa cagaatcagt
    11401 ttcatttgag tgagactcca cttgtaggtt tttggcggtc gttatggtta tgggtgaaac
    11461 agggcgcgca cgcgggccag gtcttcgggg gtgtcgaccc cgcccggcgg cgcctgctcg
    11521 accacgccca cagcaatggc gtggccgtgc cacaagacac ggagctgttc cagcgcttcg
    11581 atggcttcca gcgcggacgg cgccagttca cggtagtgac gcagaaagcc ggcgcggtag
    11641 gcatacatcc cgatatggcg cagaacgggt agctctggcg gcagcgcgtc ggttcggtgg
    11701 gcaaacgcgt cgcgggcgta gggaatcgga gcccgactaa agtaaagcgc atgaccgcgt
    11761 cggtcaagcg tcactttgac aatattcgga ttaaacatgt ccgccgcatc atggatggga
    11821 tgcgccagcg tggccatcgg cgcttcactg gcggccatca gcgcggccag gcggtcgatc
    11881 agcgccgggt cgatcagcgg ttcgtcgcct tgcacattca ccacgatgtc atcgtcggcc
    11941 agcgccagca aatcggccat ttccgccagg cggtcggtgc cggacggatg atcggcgctg
    12001 gtcagccgcg cggccacgcc atgctcggcg caggcttgca ggatgcgggc gtggtcggtg
    12061 gccaccacca cttcggcggc gccgcttttg gccgcctgct gcgccacgcg caccaccatc
    12121 ggcaggccgg cgatgtcggc cagcggtttg tccggcaggc gggtggaatg cagccgcgcc
    12181 ggaatcacca cgcgaaactc agccaccgcg cacctcttcg tcggcgggca gttcgcgcgc
    12241 ttcgctttcc agcatcatcg gaatgccgtc gcgaaccgga aaagccaggc gatcggcctt
    12301 gcagatcagt tcctgggcgg acttgtcaaa caccagcggg cctttgcaga ccgggcagac
    12361 gagaatttca agcagcttgc tgtccatggc gcagttcttt cagcgtagaa accagccagc
    12421 tcaccaaccc ggcgtccagc tgggcggtga ctggcaaaac ccagagttta ccattatcga
    12481 gcgcttgcca tttgactgcg tccttgctgg tgatcaccac ggcgtcggcg tcggcgggca
    12541 agtcagcggc ggtaaaagca tggtggtcgg caaacgccag gcagcgcgcg ggcgtcagcc
    12601 cctgctggcg cagcgtgtcg aaaaagcgtt gcgggtggcc aatgccggcc agcgccacca
    12661 cccggccagg taaatccgct ggcgcgcagc gcctggccgg ctggctgagc tggtagagcg
    12721 cgccgggctg caaacgcata tgaaagcagc gcggatgcgc cggcaagtcg ggcggcgccg
    12781 cgtcgccatt caccaccacg gcgtccaccc gcgccagccg gctgactggc tcgcgcaagg
    12841 gcccggccgg cagcaggcgg cccgtgccaa agccgcgccc gccgtccacc accgccagct
    12901 cgatgtcgcg cgccagcgcg tagtgttgca ggccatcgtc ggccaggatg acattcacct
    12961 cggggtgagc ggccagcagc gcgcgcccgg cctcgctgcg ccggcggcca atcgccacgg
    13021 caaagccggc ctgggcgtac agcaagggct cgtcgccgac ttgagccggg tcgtcgcggg
    13081 catgcaccgc ctgcgcctgg cgcgccgcgc cgccataacc ccggctgatc acgccagggc
    13141 gcaggccggc ggcgcgcagc tcggccagca aggccagggt cagcggggtt ttgccggcgc
    13201 cgccaacgtg gatattgccc accaccacca ccggcaccgg caagcgctca cggcgcagca
    13261 ggccgcaggc aaacgcggcg cggcgcaagc ccgccagcgc ggcaaacagc caggacagcg
    13321 gaagagtcca aagcgccagc cacgggtcgc ggcggccatg ccagaaggct tgcagacgct
    13381 cggcaaaagt cgaaggggag tcaggcatgg tcgggatgga tgtatatggt atataaccgc
    13441 agagcctgaa gattgattaa tgagaattga cttgtgttct ctgaatgtac actcagtctt
    13501 tgggattgcc atctttaacg cttaaggggt tggccttctg tatcaaagtt gagaactatc
    13561 gaattaaggt ttaaccgtac cttaagccaa tcttcagtcc gctgatgaat ttcttttgac
    13621 atggcaatgc gagtggtaat ggttatataa acgacctttt ctttgctctc ttcggatgtt
    13681 acagatacac cgtctgcaag taatacccta gtcacttcag gatacaattg acgaagctcc
    13741 ttagagatgc cgttggcctg cccaagaatg gcgtcacgtg cggcaagttc tgcttttaga
    13801 ttttgaactt gcgtatcccg acgtttgact gtgtcctgac tttctcgtag taaatctgac
    13861 aagagactgg atttcagcgc agtaagatcg atccgatttt ctcctgcttg atgtagcacc
    13921 actttagtgc cttcaagatt ggcagtggcc agtttagcct caatgtgatg aagtgcttca
    13981 ggactgatag gttcacctaa aatagacaag tcaatgaccc gtttatctgg atcgattttt
    14041 gtatcaacaa gttgtgattt gtcgaatgta aattctcgat caacaaactc ctgcgctttg
    14101 gatctaaaga cttcttggcg aaccaaatca agcgcaagat aaccactcgg caccgaagta
    14161 atcagggcaa gggctagcaa tgttgccttt actctctttt ctgttttttg attggcgaat
    14221 ccatgccgcc cgagtcgtaa agcccgaatg ccgactacgg tggccaaaga aataaataca
    14281 cagttgatgg catacaggta aagagcacca ccggcaaatt gccactggcc agtagcaatc
    14341 ccgaagccag cagtgcaaac tggcggcatt agtgctgttg cgatggcaac gccaggtata
    14401 acattagatt tttcttggcg agtaattccg acaataccag ctaacccgcc aaatagtgca
    14461 atcaacacat cccagatggt aggactcgta cgtgcgagca actccgattg agtttgctgc
    14521 aagggcgtca gcgagaaata taatgctgac acgacaagac taataaacgt ggcaatgccc
    14581 aagtttgtca atgaacgctt gactagatcg aagtcataaa ctgccacacc tagacctgcg
    14641 cccataatcg ggcccatcag tggtgaaatc agcatagcgc cgataataac agctgttgaa
    14701 ttgacattca gtcccactga ggctacaaaa atggcgaata tgagtaccca gggagtagct
    14761 ccacgcaact ccacaccatc acgaatacga gcttcgattt ccgtatcttc agccttgtcg
    14821 tcatgcagac tgaagcgctc tctcatgata tgcttcactc ttatcgccaa tgaaaggttt
    14881 tttttctgag attcatcatc catttacttg ctctttcctc atcacaacag gcgttcagac
    14941 tactttcaca agcacactgc ctttgccggc tttcttgact gaatacataa tttcatcagc
    15001 cttctgaatc atttccgtag ccgacagttc agcttcgtca aagaaggcga ctccaatgct
    15061 tgcgcctaca ggcgtaaatt tcgatagtgc ctcagtcaac tgcgcatgga gccgcaaggc
    15121 ctcgatttca gcgtcattcg gcgacctcga aaaggctacg acacaaaatt cgtctcctcc
    15181 aagtcttcca atcacgtcag tagatcgggt ggcattcaac aggattgtcc ccgccatctt
    15241 cagcgcggca tcaccttctt tgtgtcctcg ggtatcgttc aactgcttga agcggtctag
    15301 atcaataaac gcgatagaga acgatgcatt aaggcgatgt gctaacttga tctgctcttc
    15361 aaatgtttcc atgaaaccac gacgattcaa caagcccgta agtctatcta cccttgcatt
    15421 gttggcttct cgctgaagta gctcctgcac tttatgcacc aattcgacaa cgaccccgta
    15481 gttaatcaga tgaataaatg tatttagacc tggaatccac gtagtctcat gtgcgccggt
    15541 actgacgtga tcagcataac cccacatcaa tgctgctagt atggctgaca gccatccacc
    15601 cacccgtcca accaaccaag tattcgccaa cactggaagc agcatgagcg aagcgaacgt
    15661 gaattgagct tgcgtatgga ctcgcaatag ccccaacccg acgatgagag acgtaccgac
    15721 gagcgagaat gccaaccgca atgctcttga tgaaatgaac gaatgcccgt atgaggtatt
    15781 catgtattcg attctgtggg caacaaagat ttattaactt caattttgtg cggtgcaagc
    15841 tggatggttt ccagtggcat ctcaatattc gcggcaacga gtgccttgaa tgctgcctca
    15901 cgcaacgcaa agcgtttctc aatgtgctgc cgttcatcat ctagccatgc ctgcaattca
    15961 atcgccaagt tatagtcatt gatttcaacc acaaccatcc gaggcgccgg ctctttcaaa
    16021 tagcttggat catgacgtac gagatccagc aaaatgcttc gtacccgctc gatatcttct
    16081 gtcactgcaa tcgtgactcg cacatcaatc cgttggtgag gaaaattggt ataggaagct
    16141 atcgttttat tcatcacaga ggcattaggc acggcgagca tgcgcccatc cttggtcaca
    16201 atacgggtgg ttcgcaacgt gatgcgatct acacggccat actggccatc gatttcgacc
    16261 aaatcatcaa ttgtgaatgg ccgatccatg aaaatcagaa atccagagac gatattggac
    16321 aaggtatctt tcaatgcgaa gccgaccgtc aagcttaaca ccccggcaga cgccagcaag
    16381 gtgcctgttt gtacgcctgc cgcattaagc ccagccaaca ggcccaggga gatgaaagcg
    16441 gtcttggtca gagtctgtag cattgccgcc gtcgtactat ctacgcggcg ctttaaccga
    16501 tgactgacga atcgattgat gattatccag gtcaacatca caacactaaa cacgactagc
    16561 gccaggataa ggcggctcaa cagatcagca agtagatcaa cagaaaagac agactgtagg
    16621 cgtgatatga attgatctat caattgccgc ttctccacct gaacgattgg catcattaca
    16681 aaaacttgaa cgcaaaaacg gccacaagcg ttggcaggat cgcagcagct aacaagccca
    16741 tcggattgac agtgttgtca tcaaaatgat tgcgtagtat tgcgattcct gctgggtttg
    16801 gcgcattggc aatgatggtg aggccaccac ctgttaccgc accagccacc agcgcatatt
    16861 tgaactcgtc tgacaaacca ggcacgagcg ctccgagata tgtcaacgct gcattgtcgg
    16921 taaatgcagt tagtgctaca gcaccataaa atacttgctc gttcgtcatg cttgttagta
    16981 ctggctgcaa ccaccagcgc tgctgaccac caagaaccac gagacctgca aggaagaacg
    17041 agaccagtaa tgcttcgcgc aatataagtc gatcttgata tttttcgtat gcatgagaga
    17101 gcccaaggaa gaacaggaac aagccaagaa taaacttcgg gtgatgggaa aaaattacaa
    17161 ccgccatcaa aaaaatgaca tgagcaatag ttaggggcca gggtgtagta tgtccatttt
    17221 tcttctcatt tgacacttcg atctgtgcca attctttgca aaaaatcatg gttgcgccta
    17281 tcgagttgat gagtactgca attgcagctt tccaaccaaa attagctaac atgaatccag
    17341 tatcccactg ccaagtccca gcgaccatca aaactggcgg tgcagcaaaa ggagtgagcg
    17401 ttccaccgat tgaaatattc acaaacaaaa ccccaagcgt ggcatatttc atgcgcattg
    17461 aggctgactg agaaaatact cgacgagaaa gtatgagtgc cgccattgtc atcgcagcag
    17521 gttcggtgat aaatgaccca agcaatggaa ctgtggccaa aactacaaag taaaagccca
    17581 tagcgcctgg cagtggcata ctacttccca gtgcacgtat tgctccggtc gctgcttgta
    17641 ggataggtcg ggttgctgct atgaccatga tggcgaaaac gaatacaggt tcggtgaagt
    17701 ttcgtgattt tacgtatgca attgccgtgt ctgcaccatc gataaaaaac attgcaacga
    17761 ccaataccat tgcccagaat ccaaaaacaa cttctacctc acctagtaaa tgccatagac
    17821 cagcgtgggt tggacgatag tgagctagac gctcgaataa cttggtggca aatgtgtgaa
    17881 taatagcaat tccaaacaga gctgcaccag tccattgagt agctgtaatt gtcacactat
    17941 tcctttgcaa agttacaggg ctagaggcca ctaacaaaac catcctggca tcgttgcgtg
    18001 gctcgctatg ctgcttgtac tgtctgcggc tgttcacctc tagtagccaa tcacgttgaa
    18061 acacaaagac tcaggcaagc acctgatttc gagccttaat gccaatgatc cattcacacc
    18121 atggtgactg actactagct aggaggagcg ctgcgctgaa gtttggtcag ccgtgcttat
    18181 tgggcggccg gcgcctgggt ggcgaaggtc agcttgctca ggctggcctg gcgggcggct
    18241 tccatgacgg ttaccaccgc ctggtgcggc gccttgccgt cggcttgaat caccacgctg
    18301 gggtcggcct ggccgccagc ggcggccttg agcgcgtcgc ccagggcgtt ggcggccacg
    18361 ggctgctgat tcaccaggta ttcgccggcc gggttcaccg ccacgcggat ttccagcggt
    18421 ttttcctgcg gggcgtcggc gctggcggtg ggcaggttga tcttgagttc ggcaaagcga
    18481 ttgtaagtcg tggtgaccat caaaaagatc aagatcacca gcaacaggtc aatcagcgga
    18541 ataaaattga tttccggctc ttcgcgcttg cggcctcggc tgaagttcat gccgctctcc
    18601 ttaacggtcg cggtcgccgt ggaccacgtc caccagnnnn nnnnnnnnnn nnnnnnnnnn
    18661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    18721 nnnnnnnnnn nnnnnngaag ttcatgccgc tctccttaac ggtcgcggtc gccgtggacc
    18781 acgtccacca gatggatggc ctgttgttcc atgctcacca gcagggcgtc caccttggcg
    18841 cggaaatggc ggtaaaaaat catgctggga atcgccacca ccaggccaaa ggcggtgttg
    18901 tacagcgcca ccgagatgcc atgcgccagt tgctgcgggt tggtgccggt ggggctgctg
    18961 gcgccaaaca gctcgatcat gcccaccacg gtgcccagca ggcccagcag cggagccatg
    19021 gcggcgatgg tgcccagcgt ggtcagaaag cgctccagct cgtgggccac cacgcggccg
    19081 gcttcttcga tggcttcttt catcacttcg cggctggcgc gctggttttt caggccggcg
    19141 gccaggatgc gcgccagcgg cgcgccattg gccaattgct cgtacaatgc gcgggtgtcg
    19201 cctgcctggc tgcggttgag cacgtcttcc aagaggcccg ccggcgccac caggctttgc
    19261 cgcaggctgt agaagcgctc gaaaatgatc gccagcgata ccacggaagc cagaatgatg
    19321 gtccagatcg gccagccggc cgcttcgatg atcgacaaca cgcaagaccc cttttatgcg
    19381 tagacagtac aatctttaaa ctctacgcga aggcgtcagc tgcggcaaga catctgaccg
    19441 ccaccagggg gattttctcg gtgttttctg ggtaatttta cttaagacca tggtttgtca
    19501 cggttttttg cattcatcca cacaaactgt ggataaccat gtggacaaac cttgagtgaa
    19561 ccggcaaacc cctagtcagg caaggctttt ttctggattg cccatttgct gggcaggctg
    19621 gctaacccta taaaaaacaa atacttaaaa aataattgcg ctttcatgac aaaccctctt
    19681 gacagaatgc cagcaaggcc aaggggtgtg gatgacttga caagccagga aaaccaccca
    19741 tgcaagacgt tttacccgag ctggtttcgg tcagcgaact caaccgcctg gcgcgcggcc
    19801 tgctggagcg cggcctgccg cccttgtggg tcagcggcga gatttccaac ctcaccgtgg
    19861 ccgccagcgg gcatgcgtat ttttcgctga aagacaaagg cggccagatt cgcgcggtga
    19921 tgtttcgcgg ccgggtggcc ggcctgccgt ttcgtttgcg cgagggcgtg caagttgagg
    19981 cgtttggcca ggtgtcttta tatgaagcgc gcggcgatta tcaaatccag ctcgaccgca
    20041 tgcgcgaagc cggcttaggc cggctgtttg aagccttcga gcggctcaag cgccaactgg
    20101 gcgaagaagg gctgttcgac cccgcgcgca agcggccgct gccggcgttt ccgcgccgca
    20161 tcggcattgt ttcgtcaccg tctggcgcgg ccttgctcga cgtggtcagc acgctgcgtc
    20221 ggcgcatgcc gtcggcgcac ttggtgcttt atcccaccct ggtgcagggc gaggcggcgc
    20281 cggcgcagat tgtcgccgcc atcacccagg cggcgctgcg ccaggaagtg gacgtgctga
    20341 tggtctgtcg cggcggcggc agcattgagg acttatgggc gtttaacgac gaaaccgtcg
    20401 cccgcgcgct ggccgcctgc ccgatcccca ccatcagcgg cgtggggcac gaaaccgact
    20461 tcaccattgc cgattttgtt gccgacctgc gcgcgcccac ccccaccggg gcagccgagc
    20521 aggtgtgccc ggaccgccag gccttgctgg ccgagctggc gcagcgcgag cagcgcctgc
    20581 gccgcgccat gcagcgtctg ctgcacagcc gcatgcaagc gctggatgcc ttgtccgccc
    20641 ggctcaccca ccccggccag cgtctgcgcc agcaagcgca attgctccag cgtgatcaag
    20701 cccggctggc cagtctggcg cgcagcgccg tgcagcaggc cggctggcgg ctggagcggc
    20761 tggccgagcg tcacgctcgc ctgcgcccgc aagccgccgc cgcgcaagcc ggcctggacg
    20821 cgctggccga gcgcctgcgt caggccatgc gtcggcaact ggcgcaagcc gcgctgcatc
    20881 agcagcacct ggccgaccgg ctgagcgcgc tgaatccgca ggcggtgctg caacgcggct
    20941 acgccatcgt ggaaaaccgc aaaggccagg cggtgcgcag cccggatgcg ctcaagcaaa
    21001 gcgagcgcgt caccctgcgc ctggccgacg gcgtcaccca ggccgagatt cgccacccgg
    21061 acggcggcca acgccagctg gatttataag ccgatggcgc agccctggtt tttgtatgtg
    21121 ctggcctgtc agggcggcgg gttgtatacc ggcgtcacgc cagatgtggc ccgccgctgg
    21181 gcggcgcatc tggccggcaa gggcgcgcgc tacacccgca gctttccgcc gcagcaactg
    21241 gccctggtgg tggagttcgc cagcaagggc gaggcgctgt ccgccgagcg cgcggtgaaa
    21301 gccatgcgcg cggcggacaa acgcgcctgg ctgctcagct gtggcggcca actgccggcg
    21361 ccgtcgataa gcgcggatac ttcagtccgg ccgcttgctg cataagagtt taacagcggc
    21421 gcaccaatcc cccttggggt gcaggccagg ccatccgcgc ttgtccatta gtttaatttg
    21481 gaattaacta caattcaaaa cgtctaaaaa gttataccgc agtgggctag catgcagact
    21541 tgtttgatct gcatgaagcg agaacgccgt ggaatggggt tttgtggtgg ccggcctggt
    21601 ggtcgggttt gtggtcggtc tgaccggggt gggcggcggt tcgctgatga cgccgatttt
    21661 gctgtggttt ggcatcaacc ccgccaccgc cgtgggcacc gacctgctgt acgcctgtat
    21721 caccaagatg ggcggggtgt gggtgcatca ccagcataag cagatcgact ggcgcatcac
    21781 cggctggctg gcgctgggca gcatgccggc ggcgctgctc accgtgctgg tgctgcatgg
    21841 cctgcacctg gaaacccatt atattgagcg gctgatcaag ctgatgctgg gcgtggcctt
    21901 gatgctgacc gccatcgcca ttttgttcaa gccgtggatt ttgcgccaac tggcccgcat
    21961 ggtgccgccg cgcccggaag gtgtgctgcc ggtgaaagcc accgtgctga ccggggtaat
    22021 gctgggcgtg attgtcacct taagctcgat tggcgcgggc gcgctgggca ccctggccat
    22081 ctttgccctg tatccgctgc tgccgattgc ccggctggtg ggcaccgaga ttgcccacgc
    22141 cgtgccgctg accctggtgg ccggtctggg gcacgccagc atgggacata tggattacgg
    22201 cctgttggcg cagctgctga tgggctcgct gccgggcatc tggctgggca gccacatgac
    22261 ccgccgcgtg gccgagcaat ggctgcgccc ggcgctggcg gtgatgatgg cgatgatcgg
    22321 cgccaagctg gtgttctgag tttccgcccc atccactgaa ctgaagctgc attgcgcccc
    22381 ccgcacgcca tccatggcgg cggggggtgt gcttttgctg gtcacaccac gtctggctgg
    22441 ccaaacaccg cctcccacag cgcctgcacg tcggcgtagt ggcggcgcag cacgtcgtcc
    22501 acttccacgc tattgtgctc gttcagccgc tgggcgtgtt gcaggcggcg gtaatggcgg
    22561 tacgccgcct ggcaggcttc ggcctggtcg tgctcgatca gcccggcgtt ggcggccagg
    22621 tgcagcaggg cgatattgcc gatattgccg gtgagttccg ggtggtagtg gctgtaggcc
    22681 agcaccagat actggacgat gaactccaga tcaataatgc cgccgcgcgc gtgcttgagt
    22741 ttgtctggct gcgccgggtg ggtttccagc atgcgcgcgc gcatggtcag cacttcctgg
    22801 cgcagcgcgg ccaggtcgcg cggttgcgac agcacctcaa agcgcacttt ttcaaaggcg
    22861 tcgccaatcg ccgcgtcgcc cgccacaaag cgggcgcggg tcagcgcctg gtgttcccac
    22921 agccaggcgt cgcgcgcctg atactggcgg aaggcttcga cggtgctcac cagcaggccg
    22981 gattggccat tggggcgcag gcgcaaatcc aggtcgtaca gaatgccggc cggggtggcg
    23041 ctggtgatcc acgccgacag cttgcgcgcc agccgggcgt agccgtcggc ggcgtccggg
    23101 tgttcgtctt cgtataaaaa aatcacatcc agatcagaca catagcccag ctctttgccg
    23161 cccagcttgc catagccaat gatggcaaag cgcggggtgt cggcgtggcg cttggccacg
    23221 tcttgccagg cgtggcgcag ggtggcgtcc agaatcatgt cggccagctg cgagagctgg
    23281 tcagacaagg cctccagcgg ccacatgccg gccaggtcct gcgccaccag gcggaactgc
    23341 tgggcgtgct gcacatggcg cagggtgtcc atcttggctt ccacatcgct gccgcactcg
    23401 gccatttgct gctccagctg ggcggccagc gccggccagt ctggctcggc gtacagctcg
    23461 cgcgcgtcca gcagctcgtc cagcaggatc gggtggcggg tgaggtagct ggacacccac
    23521 gggctggacg aatacagcgt gaccagccgg cgcagggttt gcgggtattc cgccagcagc
    23581 gccagatacg gcgcgcggcg gctgatggct tccatcaggt tgatgatgcg cgccagcgtc
    23641 acatccgggt tgccgcactg ggcggagact tcaatcaagg gcggcatcag cgcgtcaaag
    23701 cgcttgcgcg tggcggcggg cagttgcaga tagcgctggc tgcgcgccag ggtggacagc
    23761 tggtgcgcca ccgctggcgc atcctgaaag ccctgcgagg ccagccgctc gaccaccgcc
    23821 tcgggcgcct cgcccacgtc cagccacagg ctggccagcg ggtggccgcc gtgttgctcg
    23881 gtgggcagca gaaacacctg ctgaaaatgc cggttgaccc gctggcgctg gcggttgagc
    23941 gcgctcacaa agccatccca atcggcaaag cccatgctgc cggccacccg ctggcgcgcc
    24001 tcgacgccgg ccggcagggt ttgcgtttgc tggtcgtcca ggtattgcag gcggtgctcg
    24061 acgtcgcgca aaaaggtgta ggcctccagc agctcgctga cggtttctgg ctccagcagc
    24121 ttgagctcgg ccagccgcgc catcgcctcg cgggtgctgc gcacttgcag gcttttttcc
    24181 cgaccgccgc gaatcagctg aaacacctgg gcgatgaact ccacctcgcg gatgccgcca
    24241 gcgcccagct tgacgttgtc ggtcagctcg cggcgcgcca cttcgcgctg aatttgcgcg
    24301 tacagctcgc gcatggcgcc gtaggcgttg taatccagat atttgcggta cacaaatggc
    24361 cggatcagcg cattcagccc ctcggcgtcg ccgcacagcg ccttgccctt gatccaggcg
    24421 tagcgctccc actcgcggcc ctggctgagc aggtagtttt ccagcgcggc aaagctcatc
    24481 accagcgggc cggaatcgcc atacgggcgc aggcgcatgt ccacgcgaaa cacaaagccg
    24541 tcgccggtgg gctcggacaa agcagcgatc agctttttgc ccagcagggt gaagtattcg
    24601 tggttgctga tatggcgggt tccgccctcg gtctggccgt cttccgggta gatgaaaatc
    24661 aggtcgatgt ccgagctgac attcagctcg ccgccgccca gcttgcccat gcccaccaca
    24721 atcagcgatt gcggctggcc gctgtcttcg ccgatgggct ggccaaagtg cgccagcagc
    24781 ggctgcacat gcgccagcgc cgtggtcacg cagaactcgg caaactggct gatggtcgaa
    24841 accacttcgt tgaggtcggc cagccggcca atgtcgcggg tgatcaggtg cgccatcatg
    24901 cgctggcgca gcttgcgcag attgcgcttg agcgcttctt cgctgtcggc gggcaggctg
    24961 gcaaagccct cttgcatgtg gcgcagcgaa aacggctcgg cggcctcaac ggcgatgcgt
    25021 tcggccaggg ccgggtcggc ggcgaacagg cgggtcaggt agtggctgtg cgcctgggcg
    25081 gcggacagcg catgaacggg gctgagtgac atataatgac catttctgaa agtgctggcg
    25141 cagggccagc cggcatttca ctgggtgaat ttccctatca tatcctgagc ggacgcctgt
    25201 tttgaacgtt ttcccccgcc tgatctccgc tcgactgctg gcctggcgcc agcgcccggc
    25261 cgtgcgccgc gcccttcgcg ccgccttatg gctgattgtg tccgctgcct tgctgctggc
    25321 ggcgctggcc tgcgccattc agttttggct gctgccgcgc ctgggcgact acaaaccggc
    25381 gctggcgcaa gccttgtcgc aagccaccgg gcagcgcgtg gacatcgacg aactgggcgg
    25441 cggctggcgg catggccatc tggtgctgtt tgtgcgcggc gtcagcgtca gccagccgca
    25501 aagcggcgcc acgcaacgct ttgccaagct ggagctggcc ccgtcgctga gctcgctgtg
    25561 gcggctggag ccgcatttca gccggattgc cctgtctggc ccggtgattc acatccgccg
    25621 cgacgcgcgc ggcgccatcc tgatcaacga catcgacctc acccagggca gcggcgacac
    25681 cgcgctgctg gattggctgc tgcgccaaag cgaagtcagc atcgaccagg ccaccctgca
    25741 ctggcgcgac gagtttgccg gcctggccga attgaccctg accgatggcc gcgtggtgct
    25801 gcggcgcggc ctgttcagcc acagcctggc cattggcgcc cgcccgccca gcgattggct
    25861 gtccagcttt gccctggcca tggactggcg cggcgaccgg ctggccgact ggaaaacctg
    25921 gcgcggccgc gtgcggctgg acgtgggcgg cgtgcgcttg tccgcctggg aaaactacct
    25981 gaacttcaac agcagccggg tgctgccgca gggcgaaggc cagagcagcg tcgagctgaa
    26041 ctttgacggc ccgcgcatcg aggcgctgaa cgcgcaactg tcgctgcgca atgtgctgct
    26101 gcggctgaac cccagcgaag ccgccgtgcg cgtgccggtg ttgtccggca agctggcgct
    26161 ggcgcaaacc cgcgatgggg tgtggacgct ggcggcgcag gatttgctgg cggtggcgga
    26221 aaacgccgcc atcttcaatc acgccgacat tcagggcgaa tggaaagacg gcgccaacgg
    26281 ccacggcaag ctgacgctgt cgcaagtgga cctggccgcc atcaagccgc tgctgcgcca
    26341 tttgccgctg gagcaaaacg ccgcctggaa ggcgctggcg cccaccggtt ttgtggaaaa
    26401 agtccaggtg ggctggcgcg gcgatattgt cgcccccagc cgctatcagg tgtcgggcgc
    26461 ttttcgccag ctgggttggc agggctcggg cgtgctgccc tcggtcaccg gcatgagcgg
    26521 cgagctcaac tttgacgaaa gccggggcga gctgaatctg cgcaaccagg gcgccgccag
    26581 cgtcaccctg cctggcgtgt ttatcgcccc gctgcaattt gccgacctca acgcccgggt
    26641 gaactggacg cgcgaggcgc agggcgtggc ggtggatttg ggcaagattc gctttgccaa
    26701 cgccgacgtc agcggcgaat tgtctggccg ctaccgctat gtgcccggcc acgccggcct
    26761 ggtggacatt caggccacgc tgggccgcgc gccggccaac cgcgtgccgg cctatttgcc
    26821 actgagcatc gaccacgaca cccgcaactg gctcacccag gcgctgcggc gcggccacgc
    26881 cgacggcgcg cagctgctgc tgcgtggcga cctggatcat tttccgtttg ccgacgccag
    26941 caagggcaat tttgaggtgc acacccgcgc cgacggcgtg accctggcct atgcgccggg
    27001 ctggccggcg ctggagaaca tcagcggcga gctgcgcttt atccgcaacc gcatggaaat
    27061 cgccaacgcc agcggccagg tgatgggcgc gcggctgttt aacgtcaatg gcgtcctgcc
    27121 agacattgaa cacagcagca gccatttgca gatcgaaggc caggccagcg ggcccaccca
    27181 ggcatttttg cagtatctgc gcgccacccc catcgaccgc gtgctgggcg ggctgacccg
    27241 cgccgcacaa acccagggcg cgggcgcgct caagctcaag ctggatattc cgctggcgca
    27301 tgccgatgcc acccaggtga gcggtgaata tcagtttctg cgcaatggcg tgcgcctggt
    27361 gggttatgcg gtgccgccct tgagcgaggt gtctggcgtg ctgcgcttta gcgaaaccgg
    27421 cgccgacagc gatggcctgc gctttaccgc actgggtggc gccggccagc tgcgcattgc
    27481 ccgcgagccg caggggcgca tgcgctttgg cctgaacggc cacgccgata tgcgcgccgc
    27541 cgcccggcaa tacctgccct ggctggcgcc acaccttaac gggcaaagcg agtatcgcgg
    27601 cgaatttcac atcagccgcg acctggacgc gctgtggctg gaatcctctt tgcgcggagt
    27661 cagcagccaa ctgccaatgc ccctggccaa gcaagccgcc gacaccttgc cgctgcggct
    27721 ggatttggcc ccagaggcgc gcggcggcct gcgcctgggc gtcaaagtgg gccagacgct
    27781 gcaaggccag tttgccctgg ctgactcggg cgaaatccag cgcggtgcgc tgcgtctggg
    27841 ggcgcacgcc tccttgccgg ccatgccggc gcgtggcggc gtggcggtgg cggtgcagga
    27901 cgagcgtttt gagcttgacc cctggctcag cctgggcagc ggcgacgccg acggcgcctg
    27961 gccgacattg agcgtggcgc tggacacccg gcaactgctg gcccagggcc gcgtgctgca
    28021 tcaggtcaag gcgcagctgc aatccagcca cggcgattgg gaaggccagg tcagcgcgca
    28081 agagctggcc ggccagctgc actggcaagc cggtgcgcat aaactcagcg ccaagctatc
    28141 gcggctgaac ctgccgctga gcagcagcgc caacgagcca aacgacacca gcctgggccg
    28201 ccaggccgcc aacctgccgg cgctggacat cacggtagat gcgctcagtt atcagcaaca
    28261 gccgctgggc catctgcaac tggccatgca gccgcgcagc aatggctggc aactggatca
    28321 gctgtggtgg agcggcccgg aaggccgcgc ccgcctgacc ggcatgtggc agcgcgacgg
    28381 ccgcgaaggc gtcaccgccc tgcgcggcca gtttgacagc caggatctgg gcggcctgct
    28441 ggcacgcttt ggctaccccg acaccctgcg ccgtggccgg ctgaccgcac aaggcgcgct
    28501 gcaatggcag ggcggcgtgc tcagcccggt attgtcctcg ctgcgcggcg acctcagcct
    28561 gcgcgccgaa aacggccagt ttgccaagat caaccctggt gtgggtcgcc tgctgggggt
    28621 gatcagcctg caatcgctgc cccggcgctt tcagctggat tttcatgaca tcttcagcga
    28681 gggctttgct tttaatgcgc tgaccggcaa ggtgaatgtg gacaatggcg tattcaccct
    28741 caacgacatg aaaatgaccg gcccggccgc cgatgtcagc ctgcgcggcc atgccgatct
    28801 gggccgtgat cgccagcaat tgcgtgtcac cgtcgagccg cacctgtcgg aaggggtggc
    28861 gctggctggc gccgcgctgc tgaatccggt gctgggcgtg gcggcgctgg ccgcgcaaaa
    28921 agtgctgcaa gacccggttg gcaagctatt tgcctatgat tacgaaatca ccggcagcct
    28981 tgctgcgccg gacatccgca aactcgaccg ctttggcggt gacgcacccc gcgagaaacc
    29041 ctgaaccatg accgcttcca ccctgaccgt tgccagcatt caaatggtgt ccgccccgga
    29101 cgtgcccagc aacctggcca ccgccgcccg cctggtggcg caggccgccg acgctggcgc
    29161 gcaactggtg gtgttgcccg agtattttgc cctgatgggg ctgaacgaca ccgataaggt
    29221 gaaggcgcgc gagccctggg gcgacggccc catccagcgc gcgctggccg atatggcccg
    29281 ccgccatcag ctgtggctgg tgggcggcac cctgccgctg gaggcccccg agccggacaa
    29341 ggtgtacaac agctgcgtgt tgtatggccc ggatggccag gcagcggcgc gctatgacaa
    29401 aatccacctc tttggctttt ctgggttggg cgagcgttat tgcgaggcgg acaccatcac
    29461 cccaggcaaa gagattgttg cggtggatac cccgctgggg cgcatgggtt tatccgtgtg
    29521 ttatgacttg cgctttccgg agctgtttcg cgccatgggc gagccggaca tcattatctt
    29581 gccggcggcg tttaccgcca tcaccggtca ggcgcattgg gagccgctgc tgcgcgcgcg
    29641 cgcgattgag aaccagtgtt ttgtgattgc cagtggccag ggtggggtgc acgccagcgg
    29701 ccgcgccacg catgggcata gtatggtgat tgatccgtgg ggggtgattg tggcggagca
    29761 ggcgcagggc gagggggtgg tgttggcgca gttggatagg cggcagttgg cgtcggtgcg
    29821 cacgcgtttg ccggcgttgg cgcatcgggt gttttgaata taaataagga gccgcgctaa
    29881 cgcgcggggt ttgattttgg gcgggagttc gtcccgcttg ccgacatcct ttctttgctt
    29941 cgccaaagaa aggatgcaaa gaaaggcgac cccaggacgc ccgcccgtcc gccgcctgcg
    30001 gcgacggtgg
//
LOCUS       DAOPYX010000002        29566 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_1556_length_29566_cov_12.714344, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000002 DAOPYX010000000
VERSION     DAOPYX010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 29566)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29566)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29566
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1556_length_29566_cov_12.714344"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            351..812
                     /locus_tag="PKN55_00135"
     CDS             351..812
                     /locus_tag="PKN55_00135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX69680.1"
                     /translation="MRVPGLYWLAFGLAAASAGADDLSYHNYAAIPDQKFACLYAYAA
                     DKTGDHETALKILDGCIAAGNVWAMIWKAMLYENGQGLPLSLEKSAELMQRGALTQDA
                     AGYGALARYHWGMALYHGRGVARQPVEGLRWLRQARDEGVPEAAAFLAALD"
     gene            930..1718
                     /locus_tag="PKN55_00140"
     CDS             930..1718
                     /locus_tag="PKN55_00140"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNX69681.1"
                     /translation="MPMTQAMDCLAILSRHLPGLARILSGDAPAATVFTRRLTWAVCL
                     LASACAGDVQREWPMPYVSRAGARQGRLDVVARFADGSVLAIEIDRANKRASVHKLQL
                     ARQRLPALALWIRWRGVAMAPPAGIQLLDLSAGGARPDVLERPTMFPTAHALLHALPD
                     ARLAEVFDTLAAAGPVRIERIVSHGQASPEGFWYDQAQAEWVMVVQGAARLAFADGEV
                     ALAAGDWLTIPAHCRHRVAWTAPDQDTVWLAVFYDEAGEQHAGR"
     gene            1705..2169
                     /locus_tag="PKN55_00145"
     CDS             1705..2169
                     /locus_tag="PKN55_00145"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNX69682.1"
                     /translation="MQADEVVLRQAGPADAPALATLIAGLSGYFLAEPDGAGAEGFLA
                     SLSVDALAACVADVRQDYWLAWRGETLVGAAALRDGCHVHHLFVAEACQRQGIAGRLW
                     RTLLGRTSAPEVTVNASVYAAPVYARWGFVATQPPQQRMGLAYVPMRWLRTA"
     gene            2184..2603
                     /locus_tag="PKN55_00150"
     CDS             2184..2603
                     /locus_tag="PKN55_00150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019921046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNX69683.1"
                     /translation="MFSHVFLGVSDFDRALAFYDALMAVLGHARRFCERERPWAGWQP
                     AGGGRPLLLIGRPFDGAAHQPGNGQMLALDAASRAQVDAAWQVALAHGGMDEGAPGLR
                     AQYHPDYYGAYFRDPDGNKLCVVCHLPVAPGLSISAD"
     gene            3109..3798
                     /locus_tag="PKN55_00155"
     CDS             3109..3798
                     /locus_tag="PKN55_00155"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HNX69684.1"
                     /translation="MISRLAHLPWFAGLPAELLTHIARHGTELSLADQQVLFLKDSPA
                     QYLALVLSGQVYHLLHNQDGRELIIDRSQVGDWVGESALLEGHDRRDCTAVADGATRV
                     WLLPRAQFGPLTGNPEFLRRMFASVCRRMRTHTEHVETLCLHQLGPRLARHLLRMVDE
                     QPHAASVPGVWLHPPKQSVLASMVNVSRPKLNAQLRNWQRNGLIHHQPNILHILDVEK
                     LRRIAYGECDM"
     gene            3972..4520
                     /locus_tag="PKN55_00160"
     CDS             3972..4520
                     /locus_tag="PKN55_00160"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNX69685.1"
                     /translation="MELAIGGRSRFENAWRSVRADLGRRARRLTHGDHDRAEELLSRT
                     ALKAFLYICQFSERVRDAEGFLFLVLNHTFLDSARRHTREEKVIDPYVDLDTDSIHAM
                     ASSAPSPLQALLMQEELEQVRQALMQLSDAQRMLFQLKFEEERPYSEIAAAFGISEAL
                     ARKRVELLRKKLHALLPQRRVH"
     gene            4645..4884
                     /locus_tag="PKN55_00165"
     CDS             4645..4884
                     /locus_tag="PKN55_00165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011134288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RebB family R body protein"
                     /protein_id="HNX69686.1"
                     /translation="MADSTPVNSQITDAVTQTNVKVVAEAPAQAMAALYQVASHSAGL
                     SLQNAVHSQQALNQISSAVISKAVTMILSVGDKAA"
     gene            5529..6113
                     /locus_tag="PKN55_00170"
     CDS             5529..6113
                     /locus_tag="PKN55_00170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011134287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX69687.1"
                     /translation="MSTDTTPDTPGGGGNSSPAPSQGSDAAAPAAGGSGGASPPKSTL
                     ATMTAKMGPPSSTPPATDALNSQIVQAVQLTNAENAAYGPNQVALAPNMMITQASGLV
                     AQSAAAYFDGASKMVLASKSVLLKDMTEKFAEQNLKGAAEDALGILITDLLMGTAAAV
                     AAAAGAIEAESAHFSLDKIDQSIEKYHSTLGSKG"
     gene            6314..7465
                     /locus_tag="PKN55_00175"
     CDS             6314..7465
                     /locus_tag="PKN55_00175"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HNX69688.1"
                     /translation="MKETAASKTHAHHAATPEVKNVLVLQGGGALGAYHIGAYQALHE
                     LGFEPDWVTGISIGALNAAVLATNKPEERLRKMVEFWEDIARPDGAAASYSGAYRAMY
                     NKSVVAKSMLQGLPNFWTPRVNVMMGMGGVEVQFANPTETSYYDTSPMLDTLRRHCNF
                     AWHNQGHGARLSLGATRVDTGKLEFFDSLNCQIGPAHALASGSLPPGFPATEINGKYY
                     WDGGCVTNTPLQAVLDDTHETADLRVFMVDLWGAHGRLPTTMDGVAWRQKEIQYASRM
                     DQHGTLRKYQKLFGRLLHSPECAGIHGLLHGWAEEVLGRRLRNIDIHTITYRHTGEES
                     SFSDAEFSAGSIKRRREMGYVDMLEKLDRVQAAEQAAQRHPADTRKVTS"
     gene            7724..8173
                     /locus_tag="PKN55_00180"
     CDS             7724..8173
                     /locus_tag="PKN55_00180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011134282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HNX69689.1"
                     /translation="MSVNQLSCSPRGRALIEKFEGLRLTAYQDMVGVWTIGYGHTGPD
                     VKAGLTITQQQAEQLLINDLARFNNGVNALVTVKINQNQFDALISFSYNLGLGSLQQS
                     TLLRLLNAGNFQAAADQFPRWDRAGGKEVAGLLARRNAERALFLTPD"
     gene            8322..8939
                     /locus_tag="PKN55_00185"
     CDS             8322..8939
                     /locus_tag="PKN55_00185"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNX69690.1"
                     /translation="MTTAPRILVVDRAPVFAAGVEAAFARMNHPVTLEHLKHLPSLDA
                     LPQPAPDLLLLEASWGAQHELGLIRQLRERHPHLRCILNVFDGKAGLAAAVRQAGGAG
                     VFNKALNPPMLVSLCLQVLQEDCFIPTAIGEGGVQLPASHAPNLTPRQQEILWLVAEG
                     QSNKQIARRLSVAEGTIKNQLYSMYRVLGVSNRTEASRWWLRTAQ"
     gene            9200..9859
                     /locus_tag="PKN55_00190"
     CDS             9200..9859
                     /locus_tag="PKN55_00190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX69691.1"
                     /translation="MTSPSPSPATGADTALDPNLQQLAETWLKRPLQPQEFAQLQAFQ
                     AAQQSAGVSPGEQAAAVLALSRANTQASVRDSLSAIHSNVTQAMQAQEKEEQAILKVV
                     EGARSLAELRPSALNGGTGVGMGGQMALSQIADRLAGLARQEVERCFNQTFMPLQNAL
                     SGVVDRLNQQSQTIAELQARQTQVPTPAQATLTPAAVAAGTAAGPQGMDAGKDTGQLA
                     G"
     gene            9959..10282
                     /locus_tag="PKN55_00195"
     CDS             9959..10282
                     /locus_tag="PKN55_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011134939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RebB family R body protein"
                     /protein_id="HNX69692.1"
                     /translation="MAIPTAVNDQITDAVTQANVKVLAESPAMAMGAIYQTMAHSTGI
                     LFENAVSAQQQQNTLTQASTNQGVMQIYSMGTMADAAATEKVGQGGIADNLTSMLTVL
                     QSFKG"
     gene            10324..10680
                     /locus_tag="PKN55_00200"
     CDS             10324..10680
                     /locus_tag="PKN55_00200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011134276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RebB family R body protein"
                     /protein_id="HNX69693.1"
                     /translation="MATTDTSTAAGAATPTAVNSQITDAVTQTNVKVLAESPAMAMGS
                     LFQTMAHSTGILFENAISAQQQQNTLTQASTNQGVMQIYSVDTMADAAGTEKVSQAGI
                     ADNLTSLLTVLQSFKS"
     gene            10716..11069
                     /locus_tag="PKN55_00205"
     CDS             10716..11069
                     /locus_tag="PKN55_00205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011134276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RebB family R body protein"
                     /protein_id="HNX69694.1"
                     /translation="MADTPSTDSSAGAPTAVNSQITDAVTQTNVKVLAESPAMAMGSL
                     FQTMAHSTGILFENAISAQQQQNTLTQASTNQGVMQIYSVDTMADAAGTEKVSQAGIA
                     DNLTSLLTVLQSFKS"
     gene            11105..11458
                     /locus_tag="PKN55_00210"
     CDS             11105..11458
                     /locus_tag="PKN55_00210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011134276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RebB family R body protein"
                     /protein_id="HNX69695.1"
                     /translation="MADTPSTDSSAGAPTAVNSQITDAVTQTNVKVLAESPAMAMGAM
                     FQAMSHSTGILFENAVSSQQQQNTLAQAASNQGVIQLYSMGTMSDAAATEKVSQAGIA
                     DNLTSMLSVLKSFRS"
     gene            11597..11917
                     /locus_tag="PKN55_00215"
     CDS             11597..11917
                     /locus_tag="PKN55_00215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011134279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RebB family R body protein"
                     /protein_id="HNX69696.1"
                     /translation="MAFPTAVNSQITDAVTQTNVKVLAEAPAMAMGSIYQSMAHSTGI
                     LFQNAVSAQQQQNILAQAAANQGVMQIYSMNTMAAAAGTEKMSQAGIADNLTSLLTVL
                     KSFR"
     gene            complement(11978..12745)
                     /locus_tag="PKN55_00220"
     CDS             complement(11978..12745)
                     /locus_tag="PKN55_00220"
                     /inference="COORDINATES: protein motif:HMM:NF041043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BPSS1780 family membrane protein"
                     /protein_id="HNX69697.1"
                     /translation="MPDHDTPSLPPPRVAVQRGWGWWRDGFHLFREQPLTMLLFGLVY
                     CIVLLGFQLLPGVGSVLAVFLGPLLTAGFLQVAARLTRAEEPRLAELFAAFRQPGVTS
                     LLGIGLWYLVMLMSVAFLVTLLAVALGLAPSPEALQAEPISQDTMDQLLTLGMMVMLA
                     ALPVAAAYWLAPALVTFEQASAAEAMWRSLRFMWRNLGAFALYLSGMAILTLAALLTY
                     GAAFIVVAPLSMLSLYACYRDLAGQGPVQIDNAGWVE"
     gene            complement(12889..13617)
                     /locus_tag="PKN55_00225"
     CDS             complement(12889..13617)
                     /locus_tag="PKN55_00225"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009119796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HNX69698.1"
                     /translation="MPRFFVDQPLIAGTELSLPDAVVRHVQVLRLNAGDALTVFNGSP
                     PDLEYPARILAVGKREVRVQLDAALAVSRESPLRLGLAQGISSGERMDFTLQKGVEMG
                     VNVFQPLATQRSIVRLSGERADKRLARWRDIILSACEQCGRNTVPELRPILSLDAWLA
                     ESANLPEARLMLSPLGSARLAELPAPTAAWLLAGPEGGLSDAEEQAALRAGWTALKLG
                     PRILRTETAALAAVAAMQQRWGDY"
     gene            13968..14759
                     /locus_tag="PKN55_00230"
     CDS             13968..14759
                     /locus_tag="PKN55_00230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HNX69699.1"
                     /translation="MQVVDQVIQTLREIAQTEVMPRFLRVGSTRKSDGTLFTEADLAT
                     QQALARRLPDILPYPVLGEEMTPEEQHALWEANPDGLWVVDPIDGTTNFIHGLPYFAI
                     SVALMRHGRSELGVIYNPVANEMFYARRGSGAYMNGIRLPLKAAPASMGDTIAAVEVK
                     YLRSGKLGTRLTSLAPFGSQRSLGASTIDWCYLAAGRYDLYLHGGQRLWDYAAGIVIL
                     EEAGGQACTLDSDDYWQGPLWHRSAVAALDPCLFEQWRRWVRNNQ"
     gene            complement(14988..15446)
                     /gene="pyrI"
                     /locus_tag="PKN55_00235"
     CDS             complement(14988..15446)
                     /gene="pyrI"
                     /locus_tag="PKN55_00235"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011133925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HNX69700.1"
                     /translation="MEHTLKVEALANGTVIDHIPAGQGRRILKLFRMSESGERIYIGF
                     NLPSRRTGKKDLIKVENITFTPAQANQLALFAPNATVNVIENFKVKEKLTLGVPDTVD
                     GMLTCPNSNCITATEPVETSFRVSEKEGEVRLKCKYCEKTFARDAFAQLR"
     gene            complement(15461..16384)
                     /gene="pyrB"
                     /locus_tag="PKN55_00240"
     CDS             complement(15461..16384)
                     /gene="pyrB"
                     /locus_tag="PKN55_00240"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003677959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HNX69701.1"
                     /translation="MSNPLYQRHVISIADLTRHDLETVIATAQQLKREPRTDLLKDKV
                     VASCFFEASTRTRLSFETAVQRLGGTVIGFDDGGNTSLAKKGETMADSVRIITSYVDA
                     FVMRHPKDGAARLASENSSVPVINGGDGSNQHPSQTLLDLFTIHETQGKLDGLTLAFV
                     GDLKYGRTVHSLAQALALFNCKFYFIAPEALAMPDYICEQLDAHGIEYQLAASIEEVV
                     PELDILYMTRVQKERFDETEFQHLKSKYKLDASMLEGAKPNLKVLHPLPRIDEIATDV
                     DATPHAYYFQQAQNGVFARQALLALVLNETV"
     gene            complement(16600..17358)
                     /locus_tag="PKN55_00245"
     CDS             complement(16600..17358)
                     /locus_tag="PKN55_00245"
                     /inference="COORDINATES: protein motif:HMM:NF025871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HNX69702.1"
                     /translation="MNAPFSRTGPLMELSSYPDWARQLVEDCAADRARVTQHVLFQRM
                     RDATLSYPIMRYFLIGVWPVIEQFPQYMAFNLLKVRYGRHPGEDLARTWLIRNLRVEQ
                     HHADYWVDWAEASDVSRDALIAGLDDPATLALSHWCWRTCEREALAISMAATHYAIEG
                     ATGDWSNLVCSTDTYAQTFPEADRKKATRWLRQHAQYDDTHPWEALDIICTLLGHEPE
                     AKQLEQLRLAICTSYRYMAMTLDRCMQMAPAKQD"
     gene            complement(17376..20207)
                     /locus_tag="PKN55_00250"
     CDS             complement(17376..20207)
                     /locus_tag="PKN55_00250"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNX69703.1"
                     /translation="MKLNWITAHRRLLKLVWPFVLTVALQVALGVFSLNILSAVRAYV
                     GGESLWSKGQKDAIYALYRYADSGHPADLVQYQQALGVIAGDRRAREALEQTEPNTDV
                     AREGFLAGGNAPSDVDNLIWLFVEFRDDEDISHVARLWREGDRLMDELNLIAIRLQEA
                     AASRTLDLDKQRALRQNIQRINEQITPLTKAFSRSLSDLARRVENLLLLINVLAAATL
                     VGLALWRANRLLSQSEGYASALRQSEERFKLAVQGSNDGIWDWDMRTGAVYFSPRYKE
                     LLGFGQDDSSLDFATLTARLHPEDRERAIEALRRHLANEAHYDVEYRMRTRTGEYRWY
                     RARGRVLRTPDGVPLRMSGSLTDATDRKEIEHALQSEKERAEITLAAIGEAVITTDTQ
                     GQIDYMNPAAEQLLSCSLTLARSKPLSSLFRLVDPLQRSHEMRLPPLQSGIQLPAISQ
                     NHHNLLLIQHDGQEISVNLTHSSIHTHDGQVEGQVLVLHDLTREHQYLASLSWQASHD
                     MLTGLVNRREFERSLLASLATADSEHDALMYLDLDQFKTVNDTCGHAAGDALLRQTAA
                     VLQQQLDPHDLLARLGGDEFGVLLSHTDLARAEHVAEALRQSVLKLNFTWGGQSFSIS
                     ASIGLVYLDQHPVTLAEVLRAADVACYMAKEKGRNRVQRYRPQDSEVSLRYDEMEWVH
                     RLHRALEEQRLCLYAQTIQPLAENSDGEHYEVLLRLRDEQGDLVPPIAFLPAAERYNL
                     MPQIDRWVVTAALRALEARHLGPDKPRLGLCTINLSGASINDEQFLDFLRDTLRNSPV
                     PTTSLCFEITETSAIASLASAARVVSELRALGCHFALDDFGTGMSSFAYLKHLPVDYL
                     KIDGSFVKDMVRDPVDRAMVEMIQRISAMMGKHTIAEYVEDLDTLNMLRACGVHYAQG
                     YGIARPQPFYDPRPVPAQIAAPDRTTTSP"
     gene            complement(20599..21921)
                     /locus_tag="PKN55_00255"
     CDS             complement(20599..21921)
                     /locus_tag="PKN55_00255"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019919934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008718 - D-amino-acid dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0055130 - D-alanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HNX69704.1"
                     /translation="MQKEATMKVVILGGGVIGVASAYYLAKAGCEVTVVERQPGPALE
                     TSFGNAGQISPGYSAPWAAPGIPLKALKWLFQKHAPLAIRADGSLYQWQWMAKMLSNC
                     NEKSYAVNKARMMRLAEYSRDCIKALRADTGIAYEGRQQGTLQVFRTQAQLDAMAKDI
                     AVLKECGVPFKVMNRAEIVATEPALARVQDKLVGALQLPNDETGDCKLFTEELARLAA
                     GLGVQFRWNTSIDALLSDGDRIAGVRCGGDTLKADHYVLAMGSYSRALLKTLSIDIPV
                     YPVKGYSLTVPITRADAAPVSTVMDETYKVAITRFDDRIRVGGMAELAGFDLSLNPQR
                     RATLEMVVGDLFPAGGDIPAAEFWTGLRPMTPDGTPIIGGTRFANLSLNTGHGTLGWT
                     MSAGSGKLLADLVTQRTPDISTEGLSIARYGQPARQAARPAQLTPRLS"
     gene            22118..22606
                     /locus_tag="PKN55_00260"
     CDS             22118..22606
                     /locus_tag="PKN55_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018150530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HNX69705.1"
                     /translation="MRTRYQGFKELDRIDRRILDILQSDGRISMTDLAERVGLSATPC
                     TERVRRLEKAGVIQGYYARLDPKAMDLPLLVFVEIKLSTKSGEIFEAFRREVLTLPHV
                     LDCHLVSGDFDYLIKARIPDMSLYRQLLGDILLKLPCANESRSYVVMEEVKETLELPV
                     SL"
     gene            22603..23760
                     /gene="earP"
                     /locus_tag="PKN55_00265"
     CDS             22603..23760
                     /gene="earP"
                     /locus_tag="PKN55_00265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018748631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0106361 - protein-arginine
                     rhamnosyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P maturation arginine
                     rhamnosyltransferase EarP"
                     /protein_id="HNX69706.1"
                     /translation="MNSPSCVSVDRPDWDIFCAVVDNYGDIGVCWRLARQLSAELGMS
                     VRLWVDDLASFARLCPSLAPSLDAQSIEGIEVRRWDAAALPAVTPAKRVIEAFACNLP
                     EGFVQAMAACQPAPVWINLEYLSAEDWVGGCHGLPSPHPSLPLRKHFFFPGFRDNTGG
                     VLGEADVSARREAQLADPAAGWAALGLTPPLAGELTVSLFCYGGERVDAWLDALLAHG
                     EPVRVLVPDGRGAAEAAHWCGQWLAPGWQGQRGAVRVQGLPFVEQREYDRLLWLCDLN
                     LVRGEDSFVRAQWAARPFVWHIYPQDDCAHLDKLRAFLALYAAELPAASAAALTDFWL
                     AWNHGVGIEPAWRALLAERAALAEHARRWAAQLAQRDTLAVNLARFCESLL"
     gene            23830..24390
                     /gene="efp"
                     /locus_tag="PKN55_00270"
     CDS             23830..24390
                     /gene="efp"
                     /locus_tag="PKN55_00270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HNX69707.1"
                     /translation="MKTAQELRAGNVFMLDGQPMVVQKTEYNKSGRNAAVVKMKMKSL
                     LSGSASEAVYKADEKFDVVVLDRKECTYSYFADPMYVFMDTEYNQYEVEADNMSDVLN
                     FLEDGMEDLCEVTFYDGKAISVELPTTIVREVIYTEPAVRGDTSGKVMKPGRLKNGFE
                     LPVAAFVDIGDKIEIDTRTGEFKKRA"
     gene            24546..24923
                     /locus_tag="PKN55_00275"
     CDS             24546..24923
                     /locus_tag="PKN55_00275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012698596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HNX69708.1"
                     /translation="MEHIKIGIIGAGETGTPLLARLFEAPFVQVVGVADLDLSQPGIS
                     LARGHGVMVTSDFMDLARLGENVDILIDVTGVPVVRESLRQYMQESGNDHTLIMHERI
                     AILLMSLFAGRLVKGKHDELEYH"
     gene            complement(25127..25468)
                     /locus_tag="PKN55_00280"
     CDS             complement(25127..25468)
                     /locus_tag="PKN55_00280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011133640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HNX69709.1"
                     /translation="MPDYRQLTQGISSSMAGLRQDIPEVMKGFSALAHAATQNGALDK
                     KTKELIALAIGVAVRCDGCIGFHMQALLKLGASKAEIEETLGMAVYMGGGPSLMYAAH
                     AMQAYDQMTAG"
     gene            complement(25519..25806)
                     /locus_tag="PKN55_00285"
     CDS             complement(25519..25806)
                     /locus_tag="PKN55_00285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012697685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HNX69710.1"
                     /translation="MRPHARAAAQLLKALAHEERLMILCNLLEGERTAGELWQRSSLS
                     QSAFSQHLAVLRGEGLIRSRKTAQTIHYALNPSVAMDVLRVLQSAFCPSQG"
     gene            complement(25939..27387)
                     /locus_tag="PKN55_00290"
     CDS             complement(25939..27387)
                     /locus_tag="PKN55_00290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX69711.1"
                     /translation="MKPLSPRSRLKGDPFLPNRFIFGDAIDQHGIEEYEYLVHTEQPT
                     FVCRLMHRAIPFDGADSEQFASAMLFDPEENVSYYTCNDGLAMTDFVFLGEPDSEPNA
                     GALQKICDEAVAAYWAIDEAYKNNPAEANEYGRRPRQLSPMQLDDTTRAHAVSELARA
                     AREAVSGPERAPQLIAHVHSTLHTGDARILPEALFALHDAPTARERLIDTARTLIAQP
                     DVARPDGSFVPYELWAIPLLYSANHAGDCWFFPRLAELERVLQKHLGIPYGKGLHVSP
                     TLFTPDMLNASGCQVLSQLAGMLDAGEAYVPGDIAAMRTAYQEGKQRFVPRMTLNWIV
                     FAVERGVLTHEPLADPNGLLDALMPEVEAALNEHIDYNEATLFAPEPLWQSLVTGTRA
                     SNQQRLAFTSLLLDKRIGLANVRAHIELMPAQGAFQLRLQGKDDDDTVETTFAWLMTP
                     DIAPNREAARAELEAVLEAHNIACDTYQDRLH"
     gene            27668..28204
                     /gene="hslV"
                     /locus_tag="PKN55_00295"
     CDS             27668..28204
                     /gene="hslV"
                     /locus_tag="PKN55_00295"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018150659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HNX69712.1"
                     /translation="MQQFDGTTILSVRRGQQVALGGDGQVTLGNIVIKSTARKVRRLH
                     NDQVLAGFAGGTADAFTLIERFEAKLQKYQGNLLISAIELAKEWRSDRALRRLEAMLA
                     VADRERTLVITGNGDVLEPEHGIAAIGSGGAYAQSAARALYENTELSPQDIVKKALTI
                     AGDICIYTNQNHVIETLD"
     gene            28272..>29566
                     /gene="hslU"
                     /locus_tag="PKN55_00300"
     CDS             28272..>29566
                     /gene="hslU"
                     /locus_tag="PKN55_00300"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016448039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HNX69713.1"
                     /translation="MTPQEIVHELDKHIIGQAAAKRAVAIALRNRWRRQQVAEPLRSE
                     ITPKNILMIGPTGVGKTEIARRLARLANAPFIKVEATKFTEVGYVGRDVDSIIRDLAE
                     VAVKEAREDAIRKNRHRAEDAAEERILDALLPPARPYPGFVGEPAANSEHSDTRQKFR
                     KMLREGKLDDKDIEIEVAQLSAHVEMIAPPGMEELTQQIQGMFANMGNGQKKSRKMKI
                     SEARKLITDEEAAKFVNEEDTKRDALRNVEQNGIVFIDEIDKVTARGETSGSDVSRAG
                     VQRDLLPLVEGTTVSTKYGMVKTDHILFIASGAFHLSKPSDLVPELQGRFPIRVELSA
                     LSVDDFVEILTNTDACLTRQYQALLATEGTELAFTDAGIRRMAEIAWQVNEKMENIGA
                     RRLHTVMEKLLEELSFAARSEQVAIDDAYVDSKLASLAQR"
BASE COUNT         5506 a         8906 c         9394 g         5760 t
ORIGIN      
        1 tgcaccacgg cgcgcagttc ctgatccaaa aacacatggc cgcgcgccca gtctatttgc
       61 cggtgagcat cgggaaagta gaactcaata aactcgggaa agtaactctc tacagcttct
      121 ttccacgggc tgtcgtagtc gtcggcggta tccatgctgg gctttcggca gtggcgaagc
      181 cgccagtgta tgactggata agggcgtaag ccatacggag gaatacggtg tttggtggcg
      241 atggctggcc gtgctttcta gcaccttttg ccagtatgcc tgcgctcagg gttgacggat
      301 gatggcctga ctctggcgtg cgtccggggg taaatcatac ggaggcggca atgcgggttc
      361 ctggtttgta ttggctggcg tttgggttgg cggcggcgtc ggcgggggcg gatgatctga
      421 gctatcacaa ttacgcggcg attcctgatc agaagtttgc ctgtttgtat gcttacgcgg
      481 cggacaagac cggcgaccat gagacggcgt tgaagattct ggatggctgc atcgctgctg
      541 gcaatgtctg ggcgatgatc tggaaggcga tgttgtatga aaacggccag ggtttgccgc
      601 tgagcctgga gaaatccgcc gagctgatgc agcgtggcgc gctgacgcag gatgcggcag
      661 gctatggggc gttggcgcgt tatcactggg ggatggcttt gtatcatggt cgtggggtgg
      721 cgcggcagcc ggtggagggt ttgcgttggt tgcgtcaggc gcgtgatgag ggagtgccag
      781 aggcggcggc gtttttggct gcgctggatt agcgccgctc accaaaccct cctggcgggg
      841 ttgcgcggcc tggccgtacg tcttgtactg tttgcagcgc gcgcctggcc aggcgcacgg
      901 cgctgagttt ggtgaagtgg tgtttgatca tgccgatgac acaggcgatg gattgtctgg
      961 ccatcctgag ccggcatctg cctggcttgg cccgtatatt gtctggtgat gcgccggcgg
     1021 cgacggtgtt tacccgccgt ttgacctggg cggtgtgctt gctggcctct gcttgtgctg
     1081 gcgatgtgca gcgggaatgg cccatgccct atgtgtcacg ggctggcgca cggcaggggc
     1141 ggctggatgt ggtggcgagg tttgcggatg gcagcgtgct ggcgattgaa attgatcgtg
     1201 ccaacaagcg tgcttctgtg cataaattgc agctggcgcg ccagcgcttg ccggcgttgg
     1261 cgctgtggat tcgctggcgt ggtgtggcga tggcgccgcc ggctggcatt caacttttag
     1321 acctctccgc cggcggcgcc cggccggatg ttttggaaag accgaccatg tttcccactg
     1381 ctcacgcctt gctgcacgcc ctgcccgatg ctcgcctggc cgaggtgttc gacaccctgg
     1441 cggccgctgg cccggtgcgg attgagcgca ttgtttcgca tggccaggcc tcgccggagg
     1501 gtttttggta tgaccaggcg caggcggagt gggtgatggt ggtgcaaggg gcggcgcgcc
     1561 tggcgtttgc cgatggcgag gtggcgctgg cggcagggga ttggttgacc attccggcgc
     1621 attgccggca tcgggtggcg tggacggcgc cggatcagga tactgtgtgg ttggcggtat
     1681 tttatgatga ggccggggag cagcatgcag gccgatgaag tggtattgcg ccaggccggg
     1741 cctgctgatg cgccagcgct ggcgacgctg attgccgggc tgagcgggta ttttttggcc
     1801 gagccggatg gcgccggggc tgaggggttt ttggccagcc tgagtgtgga tgcgctggcg
     1861 gcttgcgtgg cggatgtgcg gcaggattac tggttggcct ggcgcggcga aactctggtt
     1921 ggggctgcgg ctttgcgcga tggttgtcat gttcaccatc tgtttgtggc tgaggcgtgc
     1981 cagcgccaag ggattgccgg gcgcttgtgg cgcaccttgc ttgggcgcac ttcggcgccg
     2041 gaagtgactg tcaacgcctc ggtctatgcc gcgccagtgt atgcccgctg ggggtttgtc
     2101 gccacacagc cgccgcagca gcgcatgggg ctggcgtatg tgccgatgcg ttggctgcgc
     2161 accgcgtaaa aggagcttga gccgtgttct cgcatgtatt cttgggggtc agcgatttcg
     2221 accgcgcact ggcgttttat gatgcgctga tggccgtgtt gggccatgcc cggcgatttt
     2281 gcgagcgcga gcggccgtgg gcgggctggc aaccggcagg cggcggtcgg ccattattgt
     2341 tgattggccg gccatttgat ggcgcggcgc atcagccagg caatggccag atgctggcgc
     2401 tggatgcggc cagccgtgcc caggtggacg ccgcctggca ggtggctttg gcgcatggcg
     2461 gcatggatga aggcgctccc ggactgcgtg cccagtatca cccggactat tacggcgcct
     2521 actttcggga cccggacggc aacaagctgt gcgtggtctg tcatctgcct gtggcgcccg
     2581 gtctgtccat ttctgctgac tagactggac gattctgtcc actttggctt ctgcgggcgg
     2641 aggtgtggcc agtgtcactg gcgcgcttct gcatagccgc ttttctccgt atttctccgt
     2701 atattctgtt ctgaatagat cacagccgga cacgcaatgc tagccgatgg cattggtatg
     2761 tctgtgggct tgatgccccg gacggatggc atgcaggttg ctgtcgctgg gccgccgcgc
     2821 cgttgagtgg cgctcaaatc gaatgccaat ggaccgccac gcgccgtggt gatggcgtgg
     2881 ccccgtcgct gtgcttccgg aaaactctcc gctgttccca tgttgtcatg gcacaagcat
     2941 gcgtctgtga tgcttgtttc gcgagtgatt ggtatttctg tcgtcgtgtt cacagcgcac
     3001 atcgcgctac gtcctctgca ttcccgccaa ttcatcttga atggagtgtg tgccatgccg
     3061 ctctcccgcg cggttgccac tgagggcgcc tgcagtcagg acaacaccat gatatcccgg
     3121 ttggctcatt tgccctggtt tgccgggttg ccggccgagt tgctgacgca tattgcccgg
     3181 cacggtaccg agctgtcgct ggccgaccag caagtcttgt ttctcaagga ttcccctgcg
     3241 caatacctgg ccctggtgct gtccgggcag gtctaccacc tgttgcataa ccaggatggc
     3301 cgtgaattga tcatcgaccg cagtcaggta ggcgactggg tgggagaaag cgcgctgctg
     3361 gaaggtcatg accggcgtga ctgcaccgcc gtggcggatg gcgccacgcg ggtgtggctg
     3421 ctgccacgcg cccagtttgg ccccttgacg ggcaatcccg aatttttgcg ccgtatgttc
     3481 gcatcggtat gccgacgcat gcgtacccat accgagcatg tggaaaccct gtgtctgcat
     3541 cagttgggcc cacggctggc gcgccatctg ctgcgcatgg tggacgagca gccgcatgcg
     3601 gccagcgtgc ccggtgtatg gctgcatccg cccaagcaaa gcgtgctggc ctccatggtg
     3661 aatgtcagcc gtcccaagct gaatgcccag ttgcgcaatt ggcagcgtaa tgggttgatt
     3721 caccatcagc ctaatattct gcatattctg gatgtggaaa aactacgtag aattgcgtat
     3781 ggcgaatgtg acatgtgaat gtataaaatt tctgaatatc ttcccgtgta atgtcttgta
     3841 atatataaaa aatagatttt gtcacccagg taacagatgc atttgtcctt gttcaagaaa
     3901 ctgggattcc ttgttaactc aggagtcacg gcatggaaca ggaaatggaa atggcaatat
     3961 cggaaattcc catggaattg gccattgggg ggcgaagccg atttgaaaat gcctggcgca
     4021 gtgtgcgtgc cgatctgggg cgccgcgccc gccggctgac ccatggcgac catgatcgcg
     4081 ccgaagaatt gctgtcgcgc accgcgctga aagccttctt gtatatctgt caattctcgg
     4141 agcgggtacg cgacgccgaa gggtttttgt ttctggtgct caatcacacc ttcctggaca
     4201 gcgcccgtcg tcatacccgc gaagaaaaag tcatcgaccc ctatgtcgat ctggataccg
     4261 attccatcca tgccatggcg tccagtgccc cttcaccctt acaagccttg ctgatgcagg
     4321 aagagctgga gcaagtgcgc caagcgctga tgcagctcag cgacgcccag cgcatgctgt
     4381 ttcaactgaa attcgaagaa gaacgtccgt acagtgaaat cgccgcggcc tttggcatca
     4441 gcgaagcgct ggcgcgcaaa cgtgtggagt tattgcgcaa gaaattgcac gcattgctac
     4501 cgcaacggcg cgtgcactaa gccgggttca cagcccgccc ggcacggcgt tctgatgctc
     4561 agcgggccgg atgtgcgcag agcctgccgc gtgacgacat aactttttcg ccggccgtgg
     4621 ccggcatgca ctcaggagac ttccatggca gacagcaccc ccgtcaactc gcagatcacc
     4681 gatgcggtga cgcaaaccaa tgtgaaagtg gtagctgaag cgccggccca ggccatggcc
     4741 gcgctgtatc aagtggccag tcattccgcc gggttgtccc tgcaaaacgc ggtgcacagc
     4801 cagcaggcgc tgaaccagat ttccagcgcg gtgatttcca aggcagtgac catgattctg
     4861 tcggttggcg acaaggccgc gtaagttgca tcggccggtg cgctggcgct cgttgcgtgg
     4921 tcatgcccat cgggcctggc caccagcggg cgcacccgca gtccggatgg ccggcaggcc
     4981 attcgtaagg ccgacgcttt cccccgctga cccggccttg gcggagtgac tttttcatga
     5041 agtttcttat gaacgcactg aaaaaactgt ttggcggcaa gcagcgcgcc gacgccgatg
     5101 catcaggcag cagtggcagt ggtgtgggcc agcaggcgcc ggcacaaacc gtgccctcgc
     5161 agcaattggc cccgctgact gttccgtcgc aaagcctgcc gggccagcct gcccgtgcgg
     5221 cccggccggc gcccccggtg gagacgccag cgcctgcacc ggctccgatg gcgcaggctg
     5281 ttgcatctgc gcctgaacct gcacccgcat cgacgccaga gcccgaaccg gcgccggcat
     5341 ctgcggtatc agaggccagc gtcccgcctg ttgcaccgac agcggctgac acccctcagg
     5401 ccaaccctgt gcctgaggct gtggccccat cggcgccagc cagcccaccg ccagtgatgt
     5461 ccaaccccgc gcccgcccgg cctggcgatg cctcgcccgc gcctgcgccc accacggaga
     5521 acagccagat gtctacagat acgacccccg acaccccggg cggcggcggc aattccagtc
     5581 cggcgccgtc ccagggcagc gacgccgctg cgccggccgc aggtggatcc ggcggcgcca
     5641 gcccgcccaa atccacgttg gcgaccatga ccgccaaaat gggcccgcca tccagcacgc
     5701 cgccggccac tgacgcgctg aatagccaga ttgtccaggc ggtgcaactg accaacgctg
     5761 aaaacgccgc ctatggcccc aatcaggtgg cgctggcgcc gaacatgatg atcacccagg
     5821 cctccggcct ggtggcgcag tcggcagcag cgtacttcga cggcgccagc aagatggtgc
     5881 tggccagcaa gagcgtgctg ctgaaagaca tgaccgagaa atttgccgag cagaacctca
     5941 agggcgccgc cgaagacgcg ctgggcattc tgatcaccga cctgctgatg ggcaccgccg
     6001 ccgcggtggc cgccgctgcc ggggcgatcg aggccgaatc ggcgcacttc tcgctcgaca
     6061 agatcgacca gagcatcgag aaatatcact cgacgctggg cagcaagggc taaccgcggc
     6121 tcgcaaaacc cgccaggcat tgttgcgcga ccttgccgca tttacccggc cgcgcgcctg
     6181 gccaggcgcg cgatgctgag tgtggcgcgc gacgctgatt ccccatccgg cgggccgctt
     6241 gcgctggccc gcccttgtcg gcaacctgtc cccgccctgg ttgctttatc acccaagcat
     6301 caggagagta cacatgaaag aaaccgccgc gtcgaagacg cacgcccacc atgccgccac
     6361 cccggaagtg aaaaacgtgc tggtattgca agggggcggc gctctgggcg cttatcacat
     6421 cggcgcctat caggcgctgc acgagctggg ctttgagccg gattgggtga cgggcatttc
     6481 gattggcgcg ctcaatgccg cggtgctggc caccaacaag ccggaagagc gcctgcgcaa
     6541 gatggtggaa ttctgggagg acatcgcccg cccggatggc gccgccgcca gttattccgg
     6601 cgcgtatcgc gccatgtata acaagtcggt ggtggccaag tccatgctgc aagggctgcc
     6661 gaatttctgg acgccgcgcg tcaatgtgat gatgggcatg ggtggggtgg aagtgcagtt
     6721 cgccaacccc accgaaacca gctattacga caccagcccg atgctggaca ccctgcgccg
     6781 ccattgcaat tttgcctggc acaaccaggg tcatggcgcg cgcctgagcc tgggcgccac
     6841 ccgggtggat accggcaagc tggagttctt tgattcgctc aattgccaga ttggcccagc
     6901 ccatgcactg gccagcggtt cgctgccgcc gggttttccg gcgactgaaa tcaacggcaa
     6961 gtactactgg gacggcggct gcgtgaccaa cacccccttg caggcggtgc tggacgacac
     7021 ccatgaaacc gccgacctgc gcgtgttcat ggtggacctg tggggcgcgc atggccgcct
     7081 gccgaccacc atggatggcg tggcctggcg gcagaaagaa atccagtacg ccagccgcat
     7141 ggatcagcac ggcacgctgc gcaaatacca aaagctgttc ggccgcctgc tgcacagccc
     7201 ggaatgcgcc ggcattcatg gtttgctgca cggctgggcg gaagaagtgc tgggccgtcg
     7261 cctgcgcaat atcgacatcc acaccatcac ctatcgtcat actggcgaag aaagctcgtt
     7321 cagcgatgcc gagttctcgg ccggctcgat caagcgccgt cgcgagatgg gttatgtgga
     7381 catgctggaa aagcttgatc gtgtgcaggc ggccgagcag gcggcgcagc gccacccggc
     7441 agatacccgc aaagtgacca gctgacgcgg tgtaatcaag tgaaagtggc aggcctggcc
     7501 tgaaggcgta tgctccttgt gcgtcattgt gcataagcac gatggcgcgc cagcgtgcgt
     7561 ccatcacgtc aagtggcctg ccgtgatgaa ccccagacta tgttgcgcac gcccgcctgc
     7621 ctgggcacgc gcatggcgac catgaaaccg aatcgcggtt tcaggtgagg ccgatgcgtg
     7681 gcaggctggc tggatgtgtg gattgaattc accaggagaa accatgagcg tcaatcaact
     7741 cagctgcagc ccgcgcggcc gcgcgctgat cgaaaaattc gaaggcctgc gcctgaccgc
     7801 ctatcaagac atggtcggtg tgtggaccat cggttacggc cacacgggtc cggacgtcaa
     7861 agccggcctg accatcacgc agcagcaggc cgaacaactg ctgatcaacg atctggcccg
     7921 cttcaacaat ggcgtcaatg ccttggtgac ggtgaagatc aaccagaatc aatttgatgc
     7981 gctgatcagt ttttcttaca acctgggtct gggctccttg cagcaatcca ccctgttgcg
     8041 cctgttgaac gccggtaatt tccaagccgc cgccgatcag tttccgcgct gggaccgcgc
     8101 cggtggcaaa gaagtggctg gtctgctggc tcgccgcaac gccgaacgcg cgttgttcct
     8161 gaccccggac tgattgctcc gctgatttgt atcttgcaca cgccgcccaa ggtcacgccc
     8221 tgggcggcgt gtgcatttgt ggcggtcggg tatgggaggg atttcacagt ccgctgcgtg
     8281 tggcgttccc tgtggcactc atgtcttcta ggagccacac catgactact gccccgagaa
     8341 tcctggttgt tgaccgtgcc ccggtgtttg ctgccggtgt tgaggcagcc tttgcccgga
     8401 tgaatcatcc ggtgacactg gagcatctga agcatctgcc cagcctggat gccctgccgc
     8461 aacctgcccc ggatcttttg ctgctggaag cttcgtgggg cgcgcagcat gagctgggct
     8521 tgatccgcca actgcgcgag cgccaccctc atttgcgttg catcctgaat gtgttcgacg
     8581 gcaaggccgg tctggctgct gccgtgcgcc aggccggcgg cgccggggta ttcaacaagg
     8641 cattgaatcc ccccatgctg gtgtcgctgt gtctgcaagt cttgcaggaa gactgcttta
     8701 tccccaccgc catcggcgag gggggcgtgc agttgcctgc cagtcatgcc cccaatctga
     8761 cgccccgtca gcaggaaatc ctctggttgg tggctgaggg gcaatccaac aagcagattg
     8821 cccgccggct gtcagtggcg gagggcacca tcaagaatca gctgtacagc atgtaccgcg
     8881 tgctgggtgt atccaaccgt accgaagcat cacgctggtg gctgcgcact gcgcagtaag
     8941 agcggctaac aaaaccctcc tggcgttgtt gcccgacctt gccgtactac ccgtactgtc
     9001 tacggtcgtg cgcctagcca ggatcgctgc gctgggtttt gttagccgct ctaagcctca
     9061 ccgatactgc cccggtgtgc ctggattgcc ttaccgtgcg tgcggtcgtg ggccacacca
     9121 ggtgtgtcat gccgggggcg tgcgcgccgt gccacccgat ttcacctcat ttgcatttca
     9181 cgacaaggag ccctacccca tgaccagccc gagtcccagc ccagcgacag gtgccgacac
     9241 cgctctggat cccaatctgc aacaactggc cgaaacctgg ctgaaacgac cgctgcaacc
     9301 gcaggaattt gcccagttgc aggcgtttca ggcggcccag caaagtgccg gcgtgtcgcc
     9361 cggtgagcag gccgccgccg tgctggccct cagtcgcgcc aatacccagg ccagtgtgcg
     9421 cgattcactc agcgccattc acagcaatgt tacccaggcc atgcaggcgc aggaaaaaga
     9481 agaacaggcc attctgaagg tagtggaagg tgcgcgctcg ctggccgagt tgcgcccgtc
     9541 tgcactcaat ggcggcactg gcgtgggcat gggcgggcaa atggccttgt cgcagattgc
     9601 cgaccggctg gccggcctgg cccgccagga ggtggaacgc tgcttcaatc aaactttcat
     9661 gccgctgcaa aatgcgctga gtggcgtggt ggaccggctg aaccagcaaa gtcagaccat
     9721 cgccgaattg caggcgcgcc agacccaggt gcccactccg gcacaagcca cgctgacccc
     9781 ggctgcggtg gcggcgggga cggctgccgg cccccagggc atggatgcgg gcaaagacac
     9841 aggacaattg gctggctgag ccggtttcac gcccgtttct ggcgggcgtt ctatccggtg
     9901 agcgcgacgt gatgccgcgc cgattcctca accgacctta ccatcaagga gtgtttcaat
     9961 ggctattccg accgccgtga acgatcaaat caccgacgca gtgacccaag ccaacgtcaa
    10021 ggtgctggct gaatccccgg ccatggccat gggcgcgatc taccaaacca tggcccactc
    10081 gaccggcatt ctgttcgaga acgccgtttc ggcccagcaa caacaaaaca ccctgaccca
    10141 ggcttccacc aaccagggcg tgatgcagat ctacagcatg ggcaccatgg ccgacgctgc
    10201 cgccaccgaa aaagtgggcc agggtggcat cgccgacaac ctgaccagca tgctgaccgt
    10261 gctgcaatcg ttcaagggct aagtcgccga ctttccggtt tactcgaata aaggatgtcc
    10321 aacatggcaa ccactgatac ttccactgcc gctggcgctg caacaccgac tgcggtgaac
    10381 tcgcaaatca ccgacgcggt gacccagacc aacgtcaagg tgctggccga atcccccgcc
    10441 atggccatgg gctcgctttt ccagaccatg gctcactcga ccggcatcct gttcgagaac
    10501 gccatttcgg cccagcaaca gcaaaacacc ctgacccagg cctccaccaa tcagggcgtg
    10561 atgcagattt acagcgtgga caccatggcc gacgctgccg gcaccgaaaa agtcagtcag
    10621 gccggcattg ccgacaacct gaccagcttg ctgaccgtgc tgcaatcgtt caagtcctga
    10681 tgcccgtttt acttcatccg taaaggaaat caacgatggc tgatactcct tccaccgaca
    10741 gctcggccgg tgcgccgact gcggtgaact cgcaaatcac cgatgcggtg acccagacca
    10801 acgtcaaggt gctggccgaa tcccccgcca tggccatggg ctcgcttttc cagaccatgg
    10861 ctcactcgac cggcatcctg ttcgagaacg ccatttcggc ccagcaacag caaaacaccc
    10921 tgacccaggc ctccaccaat cagggcgtga tgcagattta cagcgtggac accatggccg
    10981 acgctgccgg caccgaaaaa gtcagtcagg ccggcattgc cgacaacctg accagcttgc
    11041 tgaccgtgct gcaatcgttc aagtcctgat gcccgtttta cttcatccgt aaaggaaatc
    11101 aacgatggct gatactcctt ccaccgacag ctcggccggt gcgccgactg cggtgaattc
    11161 gcagatcacc gacgcggtga cccagaccaa cgtcaaggtg ctggccgaat ctccggccat
    11221 ggccatgggt gcgatgttcc aggcaatgtc gcactccacc ggcatcctgt tcgagaacgc
    11281 cgtgtcctcg caacagcagc aaaacaccct ggcgcaggct gcgtccaacc agggcgtgat
    11341 tcagctctac agcatgggca cgatgtccga tgccgccgcc accgaaaagg tgagccaggc
    11401 tggtattgcc gacaatctga ccagcatgct gagtgtgctg aagtctttcc gcagctaatg
    11461 cggcgtcccg gtctggccca tccaggccgg ggccgcgtcc cgcgcggacc gaatgggggc
    11521 caccgccccg gtttcttgaa accgctcgcc cgcttgccgg gatgagcgcc gcctcatccg
    11581 atccaggagt caacaaatgg cattcccgac cgcagtgaat tcgcaaatta ccgacgccgt
    11641 cacccaaacc aacgtcaagg tgctggcgga agccccggcc atggccatgg gctcgatcta
    11701 ccagagcatg gctcattcca ccggcatcct gttccagaat gccgtgtcgg cccagcaaca
    11761 acagaacatc ctggcccagg ccgccgccaa ccagggcgtg atgcagatct acagcatgaa
    11821 caccatggct gctgctgccg gcaccgaaaa aatgagccag gccggcatcg ccgacaacct
    11881 gaccagcctg ctgactgtgc tgaagtcttt ccgctaagcg gtacgcggcg cggtgcaaac
    11941 ctgctgcccg gcaatctggc cgggcagcgg gtttttgtca ttccacccag ccggcgttgt
    12001 caatctgcac cgggccttgg ccggccaaat cccgatagca ggcatacagt gacagcatgg
    12061 agagtggcgc caccacaatg aatgccgcgc cataggtcag cagtgcggcc agcgtcagta
    12121 tcgccatgcc tgacagatac agggcaaatg cgcccagatt gcgccacatg aagcgcaggc
    12181 tgcgccacat cgcctcggcg gcgctggcct gctcgaaagt caccagcgcg ggcgccagcc
    12241 agtaggctgc ggccaccggc aaggccgcca gcatcaccat catgcccaga gtcagcaact
    12301 gatccatggt gtcctggctg atcggttcgg cctgcaaggc ttccggcgag ggcgccaggc
    12361 ccagcgccac cgccagcagg gtgaccagaa aagccactga catcaacatc accagatacc
    12421 acaagccgat gcccagcagg cttgtcaccc ccggctggcg aaaggcggca aacagctcgg
    12481 ccagcctggg ttcttctgcg cgtgtcagcc gcgcggccac ttgcaggaag cccgccgtca
    12541 gcaatgggcc cagaaacacc gccagcacgc tgcccacgcc gggcagcaac tgaaaaccca
    12601 gcagcacgat gcaatacacc agcccgaaca gcagcatggt cagcggttgt tcgcgaaaaa
    12661 gatgaaaccc gtcccgccac cagccccagc cacgctggac ggccacacga ggcggcggaa
    12721 gggagggagt gtcgtgatcc ggcatggtgg tattcctgtg caatattctg gaaggctccg
    12781 accagcaggc gctggccggg ctcacccatc tggcgcctcg ggcgggggat tgtactgtcc
    12841 acaagttgca cacaagctgt gcgaaacatg tggataactt gttgatggtc agtagtcgcc
    12901 ccagcgttgc tgcatggcgg cgacggcggc cagtgcggct gtctcggtgc gcaggatgcg
    12961 cgggcccagt ttcagcgcgg tccagcctgc acgcaaggcg gcttgttctt ctgcatcact
    13021 cagcccgcct tccgggccgg ccagcagcca ggccgccgtg ggggcgggca actcggccag
    13081 gcgggcgctg cccagcggcg acagcatcag ccgcgcctcc ggcaaattgg ccgattcggc
    13141 caaccaggcg tccaggctga ggatggggcg cagctcgggc acggtattac ggccacattg
    13201 ttcacaggct gaaaggatga tgtcgcgcca gcgtgccagg cgcttgtcgg cacgctcacc
    13261 gctgagccgc acgatgctgc gctgggtggc cagtggctgg aacacgttga cgcccatttc
    13321 cacccctttt tgcaaggtga agtccatgcg ttcgccgctg gaaatcccct gcgccagccc
    13381 cagccgcaac ggcgactcac gcgagacagc cagtgcggca tccagttgta cccgtacttc
    13441 gcgcttgccg acggccaaaa tccgggccgg gtactccaga tcggggggtg agccgttaaa
    13501 taccgtgagg gcatcgccag cattcaggcg cagcacctgc acatggcgca ccacggcatc
    13561 gggcaggctc agttcggttc cggcaatcag cggttgatca acaaaaaaac gcggcatggc
    13621 aatccgggca gcagggtaaa acgccattct gccagtttgc gtcgggtttt tctgctttcc
    13681 tggtggctgg ctgttcgatg ccgcgcatcg ctgatggcaa agcagcaaaa gcccggcttt
    13741 ttttgcgcgg aatgtgacat gttgtccaga cggcagccgc tggcttatgg cacactttgc
    13801 attgcagcat gggcgagcca tcagcaggac tgtggttgcc gccgggccac gagccaaatg
    13861 ctgcgccacg cccgcgcaga cgggtacgca cccggttcgc cgggcatggt cagcgcctac
    13921 cggcgcgcca gaatgatatt gacaagctga agagggggag tcgtgccatg caggtggtcg
    13981 atcaggtgat tcagacattg cgcgagattg cgcaaaccga agtcatgccg cgttttttgc
    14041 gtgtgggttc cacccgcaaa tcggacggca ccctgttcac cgaggctgat ctggccactc
    14101 aacaagcatt ggcgcgtcgc ctgccggaca tcctgcccta cccggtgctg ggcgaagaaa
    14161 tgaccccgga agaacagcat gccttgtggg aggccaaccc ggatggtttg tgggtggttg
    14221 atccaatcga cggcaccacc aacttcatcc acggcctgcc ctactttgcc atctcggtgg
    14281 cgctgatgcg ccacggccgc agcgaactgg gggtgattta caacccggtg gccaacgaga
    14341 tgttctacgc ccgtcgtggc agcggcgcct atatgaatgg catccgcctg ccgctgaaag
    14401 cggcgcctgc cagcatgggc gacaccatcg cggcggtcga ggtcaaatat ctgcgtagcg
    14461 gcaagctggg cacccggctg accagcctgg cgccgtttgg cagccagcgc agcctgggcg
    14521 cttcgaccat tgattggtgc tatctggcgg cagggcgcta tgacctgtat ctgcatggcg
    14581 gccagcggct gtgggactac gcggcgggga ttgtgattct ggaagaagct ggcggccagg
    14641 cctgcacgct ggacagcgac gattactggc aaggcccgct gtggcaccgc tcggcggtgg
    14701 ccgcgctgga tccgtgtctg ttcgagcagt ggcggcgttg ggtgcgcaat aatcagtgat
    14761 tgatcgtgtc ctgaatggat tcaggcgatc ggtttttgcg tgatgtgcgg aacgtgtgtg
    14821 aggcgccggc tgtggtgctg cgccgatgtg tcgcccgtaa aacgcaacgt gcccagcgct
    14881 tacggcgctg agcacgtgtc tggctcatct tcctttacct gctttatttg gcgaagcaaa
    14941 gcaagcgggt cggcggcggg gcagcatccc gcccaaaaca ggtgtgctta gcgcaattgc
    15001 gcaaaggcat cccgcgcgaa ggttttttcg caatacttgc acttgagccg cacttcgcct
    15061 tctttctcgc tcacccggaa cgaggtttcc accggctcgg tggcggtgat gcagttgctg
    15121 ttcgggcagg tcagcatgcc atccaccgtg tcgggcacgc ccagcgtcag tttttctttg
    15181 accttgaagt tttcaatcac attcaccgtg gcgttggggg caaacagcgc cagctggttg
    15241 gcttgtgccg gggtgaaggt gatgttctcg accttgatca ggtctttctt gccggtgcgg
    15301 cggctgggca ggttaaagcc gatataaatg cgctcgccgc tttccgacat gcgaaacagc
    15361 ttgagaatgc gccgaccctg cccggcgggg atgtggtcga tcactgtgcc attggccagg
    15421 gcttccacct tgagcgtgtg ttccatcatc gcgttctcca ttacacggtt tcgttgagca
    15481 ccagcgccag cagcgcctgg cgggcaaaca cgccgttctg cgcttgctgg aagtagtagg
    15541 catgcggggt ggcgtcgacg tcggtggcga tttcgtcgat gcgcggcagc gggtgcagca
    15601 ctttcaggtt gggcttggcg ccttccagca tgctggcgtc cagcttgtat ttggatttca
    15661 ggtgctggaa ttcggtttcg tcgaaacgct ctttctgtac gcgggtcatg tacagaatat
    15721 ccagctcggg caccacttct tcgatgctgg cggccagttg gtattcgatg ccgtgggcgt
    15781 ccagctgctc gcagatgtag tcgggcatgg ccagcgcttc gggggcgata aagtaaaact
    15841 tgcagttgaa cagcgccagg gcctgcgcca gcgagtgcac ggtgcggccg tatttgaggt
    15901 cgccgacaaa ggccagggtc aggccatcca gcttgccctg ggtttcgtgg atggtgaaca
    15961 ggtccagcag ggtttgcgag gggtgctggt tcgagccgtc gccgccgttg atcaccggca
    16021 cgctggagtt ttccgaggcc agccgcgcgg cgccgtcctt ggggtggcgc atcacgaagg
    16081 cgtccacata cgaggtgatg atgcgcaccg agtcggccat ggtctcgcct tttttggcca
    16141 gcgaggtgtt gccgccgtcg tcaaagccga tcaccgtgcc gcccagccgc tgcacggcgg
    16201 tttcaaacga cagccgggtg cgggtggagg cttcaaaaaa gcagctggcg acgactttgt
    16261 ccttgagcag gtcggtgcgc ggttcgcgct tgagctgctg ggcggtggca atcacggttt
    16321 ccaggtcgtg ccgggtgagg tcggcaatgg aaatcacatg tcgctggtac aggggattgc
    16381 tcatctcgga atccttgaat cacgggcgaa aaaaagcccc tgcgggcgag gggcgttccg
    16441 gggcgggcgc aacgcaaggc gggcggatgg cgcgggatgc atggcgactg actccggacg
    16501 gtggttgtcg ccattatacc tgtgtcgtcg gcgctgatag aagaggcgct ggcggacgac
    16561 agaataaatc cctgccgtcc agcccgacgt gtcagccgct cagtcttgct tggccggggc
    16621 catttgcatg cagcggtcca gcgtcatcgc catatagcgg tagctggtgc agatggccag
    16681 gcgcagctgt tcaagctgct tggcttctgg ttcatggccc agcagggtgc agatgatgtc
    16741 cagcgcttcc cacggatggg tgtcgtcgta ctgggcatgc tggcgcagcc agcgggtggc
    16801 ttttttgcgg tcggcttccg ggaaggtttg tgcgtaggtg tcggtcgagc acaccagatt
    16861 ggaccaatcc ccagtggcgc cttcgatggc gtagtgggtg gcggccatcg atatggccag
    16921 cgcttctcgc tcacaggtgc gccagcacca gtgcgacagc gccagcgtcg ccgggtcgtc
    16981 caggccggca atcagcgcat cgcgggagac atcgctggct tcggcccaat ccacccagta
    17041 atcggcgtgg tgttgttcca cgcgcaggtt gcggatcagc caggtgcggg ccaggtcttc
    17101 gcccgggtgg cggccatagc gcaccttcag caggttgaag gccatgtatt gcggaaactg
    17161 ttcaatcacc ggccacacgc caatcaggaa gtaacgcatg atcgggtagg acagggtggc
    17221 gtcgcgcatg cgctggaaca gcacatgctg ggtgacgcgg gcacggtcgg cggcgcagtc
    17281 ttccaccagt tggcgagccc agtccgggta gctggagagt tccatcagcg ggccggtgcg
    17341 agagaaggga gcgttcatga ggcaggtctc ctgtatcagg gtgaagtcgt cgtcctgtcc
    17401 ggcgccgcga tctgggcggg cacgggccgg ggatcgtaaa agggttgtgg cctggcaatg
    17461 ccataaccct gggcatagtg cacgccacag gcgcgcagca tgttcagcgt atcgaggtct
    17521 tccacgtatt cggcaatggt gtgtttgccc atcatggcgc tgatgcgctg gatcatttcc
    17581 accatggcgc gatccaccgg gtcgcgcacc atgtccttga caaagctgcc gtcgattttc
    17641 aggtaatcca ccggcaggtg cttcaggtag gcaaacgacg acatgccggt gccaaagtcg
    17701 tcgagggcaa aatggcagcc cagcgcacgc aattcgctga ccacccgcgc tgccgaagcc
    17761 aggctggcaa tcgcgctggt ttcggtgatt tcaaaacaca gtgaggtggt gggcaccggg
    17821 ctgttgcgca gggtgtcgcg caggaaatcc aggaactgtt catcgttgat cgaggcgccg
    17881 gacagattga tggtgcacag gcccagccgg ggcttgtccg ggcccaggtg acgggcctcc
    17941 agcgcgcgta gcgccgcagt gaccacccag cggtcaatct ggggcatcag gttgtagcgt
    18001 tctgcggcgg gcaggaaggc gatcggtggc accagatcac cttgctcgtc gcgcaggcgc
    18061 agcaagactt catagtgttc gccatcgcta ttttcagcca gcggctggat ggtttgcgca
    18121 tacaggcaca ggcgctgttc ttccagtgcg cgatgcagcc ggtgcaccca ttccatctcg
    18181 tcgtagcgca aggagacttc gctgtcttgc ggccggtagc gctgcacccg gttgcggcct
    18241 ttttccttgg ccatatagca ggccacatcc gctgcccgca gcacttcggc cagggtgacg
    18301 ggatgctggt cgaggtacac caggccaata ctggcgctga tcgagaagga ttgtccgccc
    18361 caggtgaagt tgagcttgag cacactctgg cgcagggctt cggccacgtg ttcggcgcgg
    18421 gccagatcgg tgtggctgag cagtacgcca aattcgtcgc cccccagccg ggccagcaga
    18481 tcatgcggat ccagttgctg ttgcagcacg gcggcggtct ggcgcagcag ggcgtcaccg
    18541 gccgcatggc cgcaggtgtc gttgacggtc ttgaactggt ccaggtccag atacatcagg
    18601 gcatcatgtt cactgtcggc tgtggccagt gaggccagca ggctgcgctc gaattcacgg
    18661 cggttgacca gtccggtcag catatcgtgg ctggcctgcc aggacagact ggccaagtat
    18721 tgatgctcgc gggtgaggtc gtgcaatacc agcacctggc cctctacctg gccatcgtgc
    18781 gtatggatgg acgagtgggt caggttgacc gagatttcct gcccgtcatg ctggatcagc
    18841 agcagattat ggtgattctg gctgatggcc ggcagctgta tgccggattg caggggcggc
    18901 agccgcattt cgtggctgcg ttgcaaggga tcgaccagcc ggaacaggct ggacagcggc
    18961 ttgctgcgcg ccagcgtcag cgaacacgac agcaattgct cggcggccgg gttcatgtag
    19021 tcgatctgtc cctgggtatc ggtggtgatc accgcttcgc caatggccgc cagcgtgatt
    19081 tcggcgcgtt ctttctcgct ttgcagcgcg tgttcgattt ctttgcggtc ggtggcgtca
    19141 gtcagtgagc cagacatgcg cagaggcaca ccatccggag tgcgcagcac ccgtccgcgg
    19201 gcccgatacc agcggtattc gccggtgcgg gtgcgcatgc ggtattccac atcgtaatgg
    19261 gcttcattgg ccagatggcg gcgcagggct tcgatggcgc gttcacggtc ttccgggtgc
    19321 agacgggcgg tgagggtggc aaagtccagg cttgagtcgt cctggccaaa tccgagcaat
    19381 tctttataac gcggcgagaa atacaccgca ccagtgcgca tgtcccaatc ccagatgcca
    19441 tcattgctgc cctgcacggc cagcttgaag cgctcctcgc tttgccgcag tgcactggca
    19501 tagccttcgc tttggctcag caggcggttg gcgcgccaga gtgccagtcc gaccagggtg
    19561 gcagctgcca gcacattgat cagcagcagc aggttttcta cccgccgtgc cagatcgctg
    19621 agcgaacgcg aaaaagcctt ggtcagcggg gtgatctgtt cgttgatacg ctggatgttc
    19681 tggcgcagtg cccgttgctt gtccaaatcc agcgtgcggc tggccgcggc ttcctgcaag
    19741 cggatggcga tcaggttgag ctcgtccatc agccggtcac cctctcgcca cagccgggcc
    19801 acgtgcgaga tatcctcatc atcgcggaat tcgacaaaca gccagatcag gttatcgacg
    19861 tcgctcggcg cattgccgcc cgccagaaat ccttcgcggg caacatcggt attgggttcg
    19921 gtttgctcca gcgcctcgcg tgcgcgccgg tctccggcaa tgacccccag ggcttgctgg
    19981 tattgcacca aatctgcggg gtggccgctg tcggcatagc gataaagggc ataaatggcg
    20041 tctttttgcc ccttggacca aagactctcg ccgccgacat aggcgcgcac ggccgacagg
    20101 atgttcagac tgaacacgcc cagcgcaact tgcagtgcga ccgtgagtac aaatggccag
    20161 accaatttca gcaggcggcg gtgtgcggtg atccagttca gtttcatgcc aatttccttg
    20221 ctggcgcggg ggcagccaag caggtcacgc ggacggagcc ctggcgggtt agacgagtca
    20281 gggcggacac aaatagcctt ggcacttgac acgtataccg ctgagcgtcg gcaatgcgga
    20341 cacggcgcca tggtgtgacc tagccgtgta gcataatgtg tcgatgtcat gcctgtatag
    20401 gtatagctgg cagaaggccg tacaccaagc gtcagatgat tcgtttgttg cttttatacg
    20461 acaaaatcat agccgggtac gcggggttgc cgctgggggc gtgcacaaaa aacggccccg
    20521 caagatggcg gggccgtggc aatggtcgct gctgggatgg cagaaccgag ggaaacgggc
    20581 agaaacagtc gggccgagtc aggacaggcg cggggtcagt tgcgccgggc gcgcggcttg
    20641 ccgggccggc tggccgtagc gggcaatcga caggccttcg gtgctgatgt ccggcgtgcg
    20701 ttgggtgacc aggtcggcca gcaacttgcc ggagccggcg ctcatggtcc agcccagggt
    20761 gccgtggccg gtgttcagcg acagattggc aaagcgggtg ccgccaataa tcggcgtgcc
    20821 atccggggtc atcgggcgca ggccggtcca gaactctgcc gccggaatgt cgccgccagc
    20881 cgggaacaga tcgcccacca ccatttccag cgtggcccgg cgttgcgggt tcagcgacag
    20941 atcaaaaccg gccagctcgg ccatgccgcc cacgcggatg cggtcgtcaa agcgggtgat
    21001 cgccactttg taggtttcgt ccatcaccgt ggacaccggc gcggcgtcgg cgcgggtaat
    21061 cggcacggtc agcgaatagc ccttcaccgg gtacaccggg atgtcgatgc tcagggtttt
    21121 cagcagcgcg cgcgagtagc tgcccatggc cagcacatag tggtcagcct tcagcgtgtc
    21181 gccgccgcag cgcacgccag caatgcggtc gccgtcgctc agcagcgcgt caatcgaggt
    21241 gttccagcgg aactgcacgc ccaggccggc agccaggcgg gccagctctt cggtgaacag
    21301 cttgcaatcg ccggtttcgt cattgggcag ttgcagcgcg cccaccagtt tgtcttgcac
    21361 gcgcgccagc gccggttcgg tggcgacaat ctcggcgcgg ttcatcacct tgaacggcac
    21421 gccgcattct ttcagcacgg cgatatcttt ggccatggcg tccagctgcg cctgggtgcg
    21481 gaacacttgc agcgtgcctt gctggcggcc ttcataggca atgcccgtgt cggcgcgcaa
    21541 ggccttgatg cagtcacggc tgtactcggc caggcgcatc atgcgggctt tgttcaccgc
    21601 gtagcttttt tcgttgcagt tggacagcat cttggccatc cattgccatt gatacaggct
    21661 gccgtcggcg cggatggcca gcggtgcgtg tttctggaac agccacttca gcgccttcag
    21721 cggaatgccc ggcgccgccc acggcgccga ataacccggc gagatttgcc cggcgttgcc
    21781 aaagctggtt tccagcgccg gccccggctg acgttcgacc acggtcactt cgcagcctgc
    21841 cttggccagg taatacgcgc tggccacccc gatcacaccc ccacccagaa tcacgacttt
    21901 catcgttgcc tccttctgta ttgcagagcg ctcaccttgt gggcgggcat gcgccagatt
    21961 ggcgcagcgc cgggcgctgc atgaaaacac attcaagaaa aggcgcttac gcaccagtga
    22021 tttgcgttag tatatgaagg cttggccggt gaatttcacc aaagaaacca taaggccagt
    22081 gacaactttg tgtgtttttt agaaaaagca gggaaacatg agaacccgat accagggctt
    22141 caaagagctc gaccgcatcg accggcgcat cctcgatatt ctgcaatctg acggccgtat
    22201 ttccatgacc gatctggccg agcgggtggg cctgtcggcc acgccgtgca ccgaaagagt
    22261 gcgccgcttg gaaaaagccg gggtgattca gggctactac gcccggctcg accccaaggc
    22321 catggatttg cccctgctgg tgtttgtcga aatcaagctg tccaccaaat ctggtgaaat
    22381 ctttgaagcc ttccgtcgcg aagtgctcac cctgccgcat gtgctggact gccatctggt
    22441 gtccggggat tttgactacc tgatcaaggc gcgcatcccc gacatgtcgc tgtaccggca
    22501 attattgggt gacatcctgc tgaaactgcc ctgcgccaac gagtcgcgca gctatgtggt
    22561 gatggaagaa gtcaaggaaa ccctggaatt gccggtgagc ctatgaacag cccctcttgt
    22621 gtcagtgtag accgccctga ttgggatatc ttttgcgccg tggtggataa ctacggcgac
    22681 atcggcgtgt gctggcggct ggcgcgccag ctgtccgccg agctgggcat gtcggtgcgg
    22741 ctgtgggtgg acgatctggc cagctttgcc cggctgtgtc cgtcgctggc gccaagcctg
    22801 gatgcgcaaa gcatcgaagg cattgaggtg cggcgctggg acgccgccgc cttgccggcg
    22861 gtcaccccag ccaaacgggt gatcgaagca tttgcctgca acttgccgga gggctttgta
    22921 caagccatgg ccgcctgcca gccggcgccg gtgtggatca atctggaata cctcagcgcc
    22981 gaagactggg tgggcggctg ccatggcctg ccgtcgccgc atcccagctt gccgctgcgc
    23041 aagcattttt tctttccggg ctttcgcgac aataccggcg gggtgttggg cgaggccgat
    23101 gtgtcggcgc ggcgcgaggc gcagctggcc gacccggccg ccggctgggc tgcgctgggg
    23161 ctgacaccgc cgctggccgg tgagttgacc gtgtcgctgt tttgctacgg cggcgagcgc
    23221 gttgacgcct ggctggatgc gctgctggcg cacggtgagc cggtgcgcgt gctggtcccc
    23281 gatgggcgcg gcgccgccga ggcggcgcac tggtgcggcc agtggctggc gccgggctgg
    23341 caagggcagc gcggcgcggt gcgcgtgcag ggcttgccgt ttgtcgagca gcgcgagtac
    23401 gaccgcctgc tgtggttgtg cgacctgaat ctggtgcgcg gcgaggattc ttttgtgcgc
    23461 gcacaatggg cggcgcggcc gtttgtctgg cacatttatc cacaggacga ttgcgcgcac
    23521 ctggataaat tgcgggcttt tctggcgctg tacgccgccg aattgccagc cgccagcgcc
    23581 gccgcgctga cggatttctg gctggcctgg aaccacggcg taggcattga gccggcctgg
    23641 cgggcgctgc tggccgaacg cgctgcgctg gccgaacacg cccggcgctg ggcggcgcaa
    23701 ctggcgcaac gtgacacgct tgccgtcaat ctggcgcgtt tttgcgaaag tcttttataa
    23761 tcacgcgttt tcgcaaatca gtggccgcgt ggcggccgct cactaccaga actgtaaaca
    23821 ggatattcaa tgaaaaccgc tcaggaactc cgcgccggca acgtgttcat gctcgacggc
    23881 cagccgatgg tcgtgcaaaa gaccgagtac aacaagtccg gtcgtaacgc cgccgtggtg
    23941 aagatgaaaa tgaagagcct gctgtccggc tccgccagcg aagccgtgta caaggctgat
    24001 gaaaagtttg acgtggtggt gctcgaccgc aaggaatgca cctactcgta ctttgccgac
    24061 ccgatgtatg tgttcatgga caccgagtac aaccagtacg aagtggaagc cgacaatatg
    24121 agcgacgtgc tgaacttcct ggaagacggc atggaagatc tgtgcgaagt gaccttctac
    24181 gacggcaagg ccatctcggt tgaactgccg accaccatcg tgcgcgaagt gatctacacc
    24241 gagccggccg tgcgtggcga cacttcgggc aaggtgatga agccgggccg cctgaagaac
    24301 ggctttgagc tgccggtggc ggcttttgtg gacatcggcg acaagatcga aatcgatacc
    24361 cgcactggcg agttcaagaa gcgcgcctga tggcgtggtt tggcatccgc caaaaaccct
    24421 gctggaaaca gcagggtttt ttcatgcccg tcatgggcgc gatccatcct aaggattgat
    24481 caatatcaac atcttatgcc ggcctttgcc ctatgcttga tacatttttt tgcaaagagg
    24541 cgggtatgga acacatcaag attggcatca ttggcgcagg cgaaaccggc acgccgctgt
    24601 tggcgcgttt gtttgaagcg ccgttcgtgc aagtggtggg cgtggcggat ctcgatctgt
    24661 cgcagccggg catttcgctg gcgcgcggtc atggcgtgat ggtgacctcg gacttcatgg
    24721 acctggcccg cctgggtgaa aacgtggata tcctgattga cgtcaccggc gtgccggtgg
    24781 tgcgggaaag cctgcgccag tatatgcagg aatccggcaa cgaccatacg ctgatcatgc
    24841 acgagcgcat cgccatcttg ctgatgtcgt tgtttgccgg ccggctggtc aagggcaagc
    24901 acgacgagct ggaataccac tgagcggtta gtggcgcagc agcaaaaaaa cgaccgaggc
    24961 cggcgcctgc ggtcgttttt ttatgtgtgc gatgcagcgt gtgtcagcgc tgttctcgcg
    25021 tcgcttacca aattcagcgc agcgatcctg gtgagacgcc ccgcagacag tgcgggtggt
    25081 gcggcaaggt cgggctagca cgccaggagg atttgcttcg cggcgcttag cctgcggtca
    25141 tctggtcata cgcttgcatg gcgtgcgccg catacatcag cgacgggccg ccgcccatat
    25201 acaccgccat gcccagggtt tcttcgattt ctgccttgct ggcgcccagc ttgagcagcg
    25261 cttgcatatg aaagccgatg cagccatcac agcgcaccgc cacgccgatg gccagggcga
    25321 tcagctcttt ggttttcttg tccagcgcgc cattctgggt ggcggcgtgc gccagcgcgc
    25381 tgaagccttt catcacctcg ggaatgtcct ggcgcaggcc ggccatgctg ctgctgatgc
    25441 cttgggtaag ttggcgatag tctggcatga ttcttcctta tctaatttag aaaaatacga
    25501 aataatgggc aaaaaaattc agccttggct ggggcaaaat gccgattgca gcacgcgcag
    25561 cacatccatg gccaccgagg ggttcagcgc atagtggatg gtctgtgcgg tcttgcgcga
    25621 gcggatcaag ccttcgccgc gcaacaccgc cagatgctgc gagaacgccg actgcgacag
    25681 gctggagcgt tgccacaatt cacccgccgt gcgttcgcct tccagcagat tgcacaggat
    25741 catcagtcgt tcttcatgcg ccagcgcctt gagcagctgg gcggcggcgc gggcatgagg
    25801 acgcatctcg gcgggcgcca acgtgttcat ggccatcaat ttagcttttt cttaaataaa
    25861 gcgcaagccc aaaacaagcg ttttaccggc aaagcgcaag gcgccttgcc ggacgggttg
    25921 agtacagcga acaaaacgtc agtgcagacg gtcttgatag gtgtcgcagg caatattgtg
    25981 cgcttccagc accgcttcca gctcggcccg tgcggcttcg cggttggggg caatgtctgg
    26041 ggtcatcaac caggcaaagg tggtttccac cgtgtcgtcg tcgtccttgc cttgcagacg
    26101 caactggaaa gcgccctgtg ccggcatcag ttcgatatgc gcgcgcacat tggccaggcc
    26161 gatgcgcttg tccagcagca aggacgtaaa cgccaggcgc tgttgattgg acgcccgtgt
    26221 gccggtgacc aggctttgcc acagcggctc gggggcaaac aaggtcgctt cgttataatc
    26281 aatgtgctcg ttcagcgcgg cttcgacttc cggcatcagc gcatccagca gaccattcgg
    26341 gtcggccagc ggttcatgcg tcagtacacc acgctcgacg gcaaacacaa tccagttcag
    26401 ggtcatgcgc ggcacaaaac gttgcttgcc ttcctggtag gcggtgcgca tggcggcaat
    26461 atcgcccggc acataggctt cacccgcatc cagcatgccg gccagctggc tgagcacctg
    26521 gcagccagag gcattgagca tgtccggggt gaacagcgtg ggcgagacat gcaggccctt
    26581 gccgtaagga atgcccaggt gcttttgcag cacgcgctcc agttcggcca aacgcggaaa
    26641 gaaccagcaa tcgccggcat ggttggcgct gtacagcagc ggaatcgccc acagctcgta
    26701 cggcacaaac gagccatccg ggcgggcgac gtccggctgg gcgatcagcg tgcgggcggt
    26761 gtcgatcagt cgctcacgtg cggtaggcgc atcgtgcagg gcaaatagcg cttccggcag
    26821 gatgcgagcg tcgccggtgt gcagggtgct atggacatgg gcaatcagct gcggcgctcg
    26881 ttccggcccg ctgaccgctt cgcgcgcggc gcgtgccagt tcggataccg cgtgggcgcg
    26941 ggtggtgtcg tccagttgca tcgggctgag ctggcgtggt cggcggccat attcattggc
    27001 ttcagccggg ttgtttttgt acgcctcgtc aatcgcccag taggccgcca ctgcttcatc
    27061 gcagatcttt tgcagcgcgc cggcgttcgg ttcgctgtcc ggctcgccca aaaagacaaa
    27121 atcggtcatc gccagcccat cattgcaggt gtagtagctg acgttttctt ctgggtcgaa
    27181 cagcatggcg ctggcgaatt gttcgctgtc ggcgccatca aacgggatgg cgcggtgcat
    27241 cagccggcag acaaaggtcg gctgctcggt gtgcaccagg tattcatatt cctcaatgcc
    27301 gtgctgatcg atggcgtcgc caaagatgaa gcggttcggc aagaaaggat cgcccttgag
    27361 gcgggaacgg ggtgacaacg gtttcatggt cggcaatcac acgctgccag catggcaacg
    27421 aaacaatagg ttaacgggca catttgcgcc ctgacatcat cgttggatta tacgcatccg
    27481 gtgcggcgaa cgtcgcctgc cacgcggcgc tggcgatctc tatatatagt gatgatgcca
    27541 cagcgcacaa gatgcccctg ccttgggcag gggtttgcct tgtaattgcc gcaactggcc
    27601 acatatcctg aaagctgccg acgtctgaat ttcggcggct ttttgttttt ttccggaagg
    27661 cgcacgcatg caacaattcg acggaaccac cattttgtcg gttcgccgcg gccagcaggt
    27721 ggccctgggc ggagacggcc aggtgacgct gggcaatatc gtgatcaaat ccaccgcgcg
    27781 caaagtgcgc cgcctgcaca atgaccaagt gctggccggg tttgccggcg gcaccgccga
    27841 tgcctttacc ctgatcgagc gctttgaagc caagctgcaa aaataccagg gcaatctgct
    27901 gatttccgcg attgagctgg ccaaagaatg gcgcagcgac cgcgccctgc gccggctgga
    27961 agccatgctg gcggtggccg accgtgagcg caccctggtc atcaccggca acggcgatgt
    28021 gctggagccc gaacacggca ttgccgccat tggctcgggc ggcgcctacg cccagtcggc
    28081 ggcgcgcgcc ttatatgaga acaccgagct gtcgccgcaa gacatcgtca aaaaggcgct
    28141 gaccattgcc ggcgacatct gtatctatac taaccagaat catgtgattg aaaccctgga
    28201 ttgacgcgcg ccgagcgcca gcctgaattt tcgccactgt caccgccggg attgccgcca
    28261 tgtctgaatc catgaccccc caggaaatcg tccacgaact ggacaaacac atcatcggtc
    28321 aggccgccgc caagcgcgcg gtcgccatcg ccttgcgcaa ccgctggcgc cgccagcaag
    28381 tggccgagcc gctgcgcagc gaaatcaccc ccaagaacat cttgatgatc ggcccgaccg
    28441 gcgtgggcaa aaccgaaatc gcccgccgcc tggcccgttt ggccaatgcg ccgttcatta
    28501 aagtggaagc caccaaattc accgaagtcg gttatgttgg ccgcgacgtg gacagcatca
    28561 tccgcgacct ggccgaagtg gcggtcaaag aagcgcgtga agacgccatt cgcaaaaatc
    28621 gccaccgcgc cgaagacgct gcggaagaac gcatcctgga cgcgctgctg ccccccgccc
    28681 gcccctaccc cggctttgtc ggcgagccgg ccgccaacag cgagcacagc gacacccggc
    28741 agaaatttcg caagatgctg cgcgaaggca agctggacga caaagatatc gaaatcgaag
    28801 tcgcccagct cagcgcccat gtcgaaatga tcgccccgcc gggcatggaa gagctcaccc
    28861 agcaaatcca gggcatgttc gccaatatgg gcaacggcca gaaaaagtcg cgcaagatga
    28921 aaatcagcga agcgcgcaag ctgatcaccg acgaagaagc cgccaaattc gtcaacgaag
    28981 aagacaccaa gcgcgacgcg ctgcgcaatg tcgagcaaaa cggcatcgtc tttattgatg
    29041 aaatcgacaa agtcaccgcc cgtggcgaaa cctccggttc ggatgtctcc cgcgccggcg
    29101 tgcagcgcga cctgctgccg ctggtggaag gcaccacggt gtccaccaaa tacggcatgg
    29161 tgaaaaccga ccatatcctg tttattgctt ctggcgcctt ccatctgtcc aagccgtctg
    29221 atctggtgcc cgagctgcaa ggccgcttcc cgatccgcgt tgagctgtcg gcgctgtcgg
    29281 tagacgactt cgtggaaatc ctcaccaaca ccgacgcctg cctgacccgt caatatcaag
    29341 ccttgctggc caccgaaggc accgaactgg cctttaccga cgccggcatt cgccgcatgg
    29401 ccgagattgc ctggcaggtg aacgagaaga tggaaaacat cggcgcccgt cgtctgcaca
    29461 cggtgatgga gaaactgctg gaagaactgt cgttcgcggc gcgctcggaa caggtcgcca
    29521 tcgacgacgc ctacgtggac agcaagctgg cctcgctggc gcagcg
//
LOCUS       DAOPYX010000003        29526 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_1560_length_29526_cov_8.304435, whole genome shotgun sequence.
ACCESSION   DAOPYX010000003 DAOPYX010000000
VERSION     DAOPYX010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 29526)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29526)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29526
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1560_length_29526_cov_8.304435"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            373..1791
                     /gene="dnaB"
                     /locus_tag="PKN55_00305"
     CDS             373..1791
                     /gene="dnaB"
                     /locus_tag="PKN55_00305"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012697209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HNX69714.1"
                     /translation="MTDYIPNPPPSDAEYQQLRMPPHSLEAEQSVLGGLLLDNSAWDR
                     IGDAVADEDFYRHDHRLIFQAIVKLIERSHPADVVTVSEQLEKSAQLGEVGGLAYLAT
                     LAQNTPSAANIKRYAEIVRERSIMRQLAQIGTEIADSAYNPMGRDARELLDEAETKVF
                     RIAESTARSQQDFLNMEQILTEVVERVNELYGRDNLDDVTGIPTGFVDLDKKTSGLQK
                     GDLVIVAGRPSMGKTAFSMNIAENVALNTGLPVAVFSMEMGATQLAMRMIGSVGRLDQ
                     HALRTGQLSDEEMDKFGMAVSRLWEAPLFIDETPGLSGLELRSRARRLARKMGGQLGL
                     IVIDYLQLMSGSSDRRDQNRASELGEISRSLKALAKELQAPVIALSQLSRSVEQRTDK
                     RPMMSDLRESGAIEQDADLIVFMYRDEYYNPDSQFKGMAEAIIGKQRNGPTGRVPLTF
                     LGHYSRFENASPQQVSGWDQGD"
     gene            1794..2114
                     /locus_tag="PKN55_00310"
     CDS             1794..2114
                     /locus_tag="PKN55_00310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012697210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HNX69715.1"
                     /translation="MSYFARHVFLCTNQRAEGESCCNALGASEALDHAKGRIKSMGLA
                     GPGKVRFQKAGCLERCGEGPVLVVYPEAVWYTYVDKTDIDEIIDEHLVNGRVVERLKL
                     SDHA"
     gene            2107..2727
                     /locus_tag="PKN55_00315"
     CDS             2107..2727
                     /locus_tag="PKN55_00315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003778099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNX69716.1"
                     /translation="MLKLQQEPIYLDGPVGQLETLVIAPKDGAPRGIALIAHPNPLQG
                     GTNTNKVVQTTARALSQHGYICYCPNLRGVGNSGGEHDYGHGEVDDAQAVVDYARAQH
                     GDVPLVLAGFSFGSYVAAQLRQRIDARHLIMLGAAVSPKYAMPHVPADTIVIHGEHDE
                     VISLAQVLDWARPQSLPVVVFPGVGHFFHGKLVLLQQYVQRLVPSL"
     gene            2740..4158
                     /locus_tag="PKN55_00320"
     CDS             2740..4158
                     /locus_tag="PKN55_00320"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HNX69717.1"
                     /translation="MTLDGPAPARLYQQVFRHWRDAIASGRLPAGARLPAVRELAARH
                     NVSLTTARRVVEELHAGGWIETRPRLGLFVAQPRQPGYPSPAGQPAPVSIATLALRLF
                     EALRDPAIAPFGSPFIDPAWLDIAGVNDHLRRAVRKFSPARGLTDLPPGHDALRQQIV
                     RRALHHGFRLQQDELLITCGAMEALNLALKSVARPGAVVAVESPAFYGMLYAIEANGM
                     QALEIRTDPRTGLDVDDLAQALGRHRIAACMLMPSLQNPLGAIMPAAARARLLALAGE
                     HDFAIIEDDAYRELLPPGGSPPPLKHHDSDGRVLYCGTFAKAFSPAPRIGWLAAGRHT
                     ETALLHKLMNTLSTSIPNQMAVASYLRDSRPDRRLARLRDTLASNRARLADTVRRHFP
                     AGSEVNTPAGGFFLWVTLPLGCDTLAHYPKALAAGVSFVPGALFSPSGRFGHCLRLNA
                     GFALDEPVLARLAEVGRQLQAG"
     gene            4222..4803
                     /locus_tag="PKN55_00325"
     CDS             4222..4803
                     /locus_tag="PKN55_00325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNX69718.1"
                     /translation="MAQDSSRADFWETRYQQGVIPWDAGQMPSQFVAFAAARPPGERV
                     LAPGCGSGYEVRGLAEHGAKVLAVDFSAAAVARAREVLGEHADCVREGDFFQLDDAPF
                     DWLYERALLCALPPKTWDDWAAQTARLVRPGGVLAGYFFIADTPKGPPFGISEARLVE
                     LLGGHFTLESREPASDSLPVFAGREHWMVWRRR"
     gene            4898..5368
                     /locus_tag="PKN55_00330"
     CDS             4898..5368
                     /locus_tag="PKN55_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018748141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016531 - copper chaperone activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone PCu(A)C"
                     /protein_id="HNX69719.1"
                     /translation="MKRLLALALFAAASLAHAHGYAVGELKIDHPWARATPGAAKNGA
                     AYLTVSNTGKQADRLVAASVDVADHAELHTHLNDNGVMKMRQIPDIPVEAGATVKLQP
                     GGLHIMLMDLKQPLKEGDKIPMTLRFEKAGEVKVDVHVDKVGAMPAAAGAHAGH"
     gene            5632..6207
                     /locus_tag="PKN55_00335"
     CDS             5632..6207
                     /locus_tag="PKN55_00335"
                     /inference="COORDINATES: protein motif:HMM:NF025595.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4337 domain-containing protein"
                     /protein_id="HNX69720.1"
                     /translation="MSGHGFHVHGPHDHAVEHGGHSGDSFASRIAVMTAVMSTVGALF
                     SYSGGATQNEAMLMKNAAAIKKTEAANQWNYYQAKSNKQNLAELALSLSQDAANKDKY
                     QKDIERYRAEKAEIKAAAEKLEAESQGFEHKSDEAMHLHHRWAQAMTGIQVAISLAAV
                     SLLTRRRWLNYATYLAAGAGVVLGGMALAHL"
     gene            complement(6240..6800)
                     /locus_tag="PKN55_00340"
     CDS             complement(6240..6800)
                     /locus_tag="PKN55_00340"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018150366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HNX69721.1"
                     /translation="MRISVPLAHAYRLLNHGPLTLISSAHADQRNIMAAAWAMPVDFQ
                     PPKVAVVIDKSSHTRQLIEASGHFVLALPGQQLAHTVLQLGSHSGRDIDKFAAFGLHG
                     ASAEHGGGLLLDGCAAWLECKVLPEPAMAERHDMFLAEVLAAWADDQAWCDGRWTFAD
                     PALRTLHYVAGGQFFVTGDSLVIEQS"
     gene            6982..8376
                     /locus_tag="PKN55_00345"
     CDS             6982..8376
                     /locus_tag="PKN55_00345"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNX69722.1"
                     /translation="MAAPASSHGMIEALASTLLDVQALIDAAPASSMLLDRDGRILTI
                     NRAGAARLGLAPAQALGLYLFDLFPVEVAASRQRRLQAVTASRQPQVFEDHRDGQHYR
                     TSAVPVMDRDGEVCRVAIFSEDITAQHEAERLLRDSEAHYRFLAENSEDVIWQLDRDM
                     HITYISAADQRLRGVARGAVLGQSVFSLFSPRGQAILARVVAERQQCLEQGLPLPPTL
                     RFEAPQQCANGEEVWVEVVSTRIDDAHGVLCGYIGMTRNVAERRRYQAMLEDANRQLR
                     EQLDKVSALQAQLQARAHRDALTGLYNRHYLHETLPDLLAQARQARHGLALVMVDLDH
                     FKQINDRHGHVAGDLAIQTAARLLIERAPSRSLCCRFGGEEFLVALCGLTPAAVCAWA
                     ENWCAELADCVLSHQGVRIALTASLGVAWQPQHGDSEAQLLERADQALYLAKRRGRNQ
                     VCVWAADEVCALRS"
     gene            complement(8429..8989)
                     /locus_tag="PKN55_00350"
     CDS             complement(8429..8989)
                     /locus_tag="PKN55_00350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX69723.1"
                     /translation="MHPDAPLWVSLLLLPAFAARGVWRVLRSGDGVALAMLIASWFML
                     AIGFKLLRPSLAVSALWLPCFYPYLWQGVFAGGWLLCRPDPLALPARQVLASDALALA
                     LGHLGVLAGGLFSQDIRHAYWYRPAAMTLVFWLASLLLQLYRVRSHRNHASALALACQ
                     LILPALLAAGVGWLARGGRPTFGPWF"
     gene            complement(9004..9663)
                     /locus_tag="PKN55_00355"
     CDS             complement(9004..9663)
                     /locus_tag="PKN55_00355"
                     /inference="COORDINATES: protein motif:HMM:NF016243.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 family protein"
                     /protein_id="HNX69724.1"
                     /translation="MSMTASEVAAYLEAHPEFFDQHSELLTQVRVPHARSEYAVPLVE
                     RQVLALRDKNRQLGAKMSQLIRFGEHNDQTITRLHTLSVQLVRTEEATDAIHRTLSAL
                     EKDFDITHAGLRLWGTATESLGEWHRQDPRLAIIARNLEHPYTGPFAAEHIIEWFPRE
                     PVLQSFGQVALRRDNGEAFGLLVLASADSYRFTTDMETDYLANLGALLSAALQRTLAM
                     H"
     gene            complement(9736..10569)
                     /gene="dapF"
                     /locus_tag="PKN55_00360"
     CDS             complement(9736..10569)
                     /gene="dapF"
                     /locus_tag="PKN55_00360"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008952731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HNX69725.1"
                     /translation="MILNFSKMHGLGNDFMVIDGVRQRVRLSAAQIRQLGDRHTGVGF
                     DQLLLVEEPALPDTDFRYRIFNSDGGEVEQCGNGARCFARFVFDQGLTDKHAIRVETA
                     KGIITPVLTDNGLVTVDMGQPRLLPVEIPFEADADAVIHPLTLPDGAELGVTVVSMGN
                     PHAVQVVDDVNTAPVAAHGPQIEQHARFPQRVNAGFMQVLNRQEIALRVYERGAGETL
                     ACGTGACAAVVAGIRRGLLDAAVTVHTRGGDLRIEWAGPGQPVRMTGPAVTVYQGSIT
                     L"
     gene            complement(10727..11233)
                     /locus_tag="PKN55_00365"
     CDS             complement(10727..11233)
                     /locus_tag="PKN55_00365"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003821846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HNX69726.1"
                     /translation="MSTLALIVAMARNRVIGKDNTLPWHLPEDLQHFKRTTLGAPIIM
                     GRRTWDSIGRPLPGRRNIVVSRNPDWQTAGAEHARSLPDALALCAAADSPAPRVFVIG
                     GAQLYAQALALADELFITEIDADIDGDAYFPALDASQWQALSRDSHVRDSDGLGYHFV
                     HLRRRPAG"
     gene            complement(11230..12024)
                     /locus_tag="PKN55_00370"
     CDS             complement(11230..12024)
                     /locus_tag="PKN55_00370"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HNX69727.1"
                     /translation="MQAYLDLMRHVLEHGHVKTDRTGTGTRSIFGHQMRFDLAKGFPL
                     VTTKKVHLKSIIHELLWFLAGDTNIAYLKAHGVSIWDEWADANGELGPVYGYQWRSWP
                     APDGRHIDQISQVLRQLRDTPDSRRMIVSAWNPALVDQMALPPCHSLFQFYVADGKLS
                     CQLYQRSADIFLGVPFNIASYALLTLMVAQVCQLQPGEFIHTLGDAHLYSNHLEQAQL
                     QLSREPRPLPVMRLNPAVTDLFTFRFEDFTLEGYDPHPAIRAEVAV"
     gene            12128..13294
                     /gene="flhB"
                     /locus_tag="PKN55_00375"
     CDS             12128..13294
                     /gene="flhB"
                     /locus_tag="PKN55_00375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008954965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
                     /protein_id="HNX69728.1"
                     /translation="MSEESDLEKTEEPSEQKLRQAREDGQVPRSRELATFAVTMTGVG
                     LLVVLGNRIVDSINTIIRREFTFDQKTLLQEGLINEHFRQGMFDMLLVLAPILGALMV
                     AAIGPSLLLGGWNFTLNSIGFKFNKLNPAPGIKRMFSVAALMEGGKAVLKSTLIGGVS
                     TWIIWREMGEIMQLITLPLDESIAQTMALTVHTTFLVTSTLLLLVLIDVPFQWWNFRK
                     GLRMTKEEVKQENKNSEGSPEVKGRIRQLQREAARKRMMGAVPEASVVVTNPTHYAVA
                     MKYEDGMGAPKVLAKGKLRTAERIIELAKEHKITIMRAPPFARALYHNTEIGQEIPHQ
                     LYTAAAQILAYVFQLKHYNAHGGMAPVYPDDLPVPRELDPESKTPQDTSPTPKS"
     gene            13305..15389
                     /gene="flhA"
                     /locus_tag="PKN55_00380"
     CDS             13305..15389
                     /gene="flhA"
                     /locus_tag="PKN55_00380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014088182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HNX69729.1"
                     /translation="MDKLSQFLTRYNTTSLAGPVLIIMILGMMILPLPAFLLDIFFTF
                     NIAVSIIVLMVGLNTRQPLEFSAFPAVLLVTTLLRLSLNVASSRMILLNGHNGADAAG
                     KVIEAFSHFLVGSNYAIGIVVFIILTIINFVVITKGAGRIAEVSARFTLDAMPGKQMA
                     IDADLNAGLIGEEDAKKRRAQIAEEANFFGSMDGASKFVRGDAIAGIMIMLINIVGGL
                     SVGLIQHDLPLGKALETYTLLTIGDGLVAQIPGLIISTAAGIVVSRVGTEKDLSEQML
                     GQMFANSQVLYITAAVIGLIGIIPNMPHVAFLLIASSLAGLAWALERSKQRAAEAEFN
                     EPDLADAQQLTETPLAEVGWRDVQAVDPVGLEVGYRLIPLVDRAQDGELLRRVRGIRK
                     KIAQELGFLVPSVHIRDNLEIRPNEYRIMLKGVEVGQGEAFVGQYLAINPGRVLGTIQ
                     GTPTTDPAFGLPALWIEGALRDEAQSLGYTVVDASTVVATHISNILSNHAAELLGREE
                     TQQLVDHVAKDAPKLVEDLIPKVVPINILQKVLQNMLTEGLHIRDIRTIIETLAEHTP
                     ITQDVDDLTSAVRVALGRTIVYQLYGNANELPVMTLEPGLEQILINAVQSKGGGLEPG
                     LAENLLASTAREAERVEQQGQVPVLLTPAALRPLLAKFLRRAMPQLAVLAHNEIPDNK
                     TIRIISVVGGRE"
     gene            15396..16964
                     /gene="flhF"
                     /locus_tag="PKN55_00385"
     CDS             15396..16964
                     /gene="flhF"
                     /locus_tag="PKN55_00385"
                     /inference="COORDINATES: protein motif:HMM:TIGR03499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhF"
                     /protein_id="HNX69730.1"
                     /translation="MVVKKFFGATTRDALKQVRNELGEDALILSNRPVGGGVEIMAVA
                     DADVSALASSMKPGAAAAVAAPVPTPGKGPAGAAPATAGSKPASSMSSAVARTYAMPV
                     EPLDDPTPSRPATTETFSTQARSSAPEPAPRPVPSISIPMSALTATTPPPPPPPPSPQ
                     SVYARYEAPAETAPAASDSSERFTEDSLPDLGGQAGAANSDEALEKLDSLSGEVREMR
                     TMLQSQLASLAWANMEEHHPRQAELFRQMLALGLSPALCRQLVAKLPDNLGDEAALKW
                     AKLALVHNLKTIDEQDEIIARGGVYALVGPTGIGKTTTVAKLAARATLRHGTERIALI
                     TTDSYRIGAQDQLRLYGKILGVSVFSVQNEADLHVTLADLADKHLVLIDSTGMGQRDA
                     RVDAQTQMYAHTAANGGRPISRVLVLAANAEGATLEDVVNRYLPGGGLVGCVMSKIDE
                     APTLGGAIDTIIRHRLPLLYVTNGQRVPEDLHLAKSNYLIDRAFKMAREASPYSLKSD
                     EYQLLTSAFVGTLN"
     gene            16975..17838
                     /locus_tag="PKN55_00390"
     CDS             16975..17838
                     /locus_tag="PKN55_00390"
                     /inference="COORDINATES: protein motif:HMM:NF018290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase operon protein YhjQ/BcsQ"
                     /protein_id="HNX69731.1"
                     /translation="MEDQAASLRRLTPAAQVGRSFAFLGVSGCGVTTMVSEMAAGLTA
                     AQRRTLIIDAHPGNPLAQRFVDDPGVTLDMVASSQGSLHDAVTQLPFGASVMNVHSRP
                     STLMHLPEPIEHRLVSEYEALTRDIELVLIDAPSVSTDPALAALADNLVLVLTPDIQT
                     LMQAYAMVKRLALEFARRRFDVLVNRARDMNEAQAIFQRLSQVTSEFLGVSLRWVGFV
                     PEDPMVRRAVLLRTTTLHGFPGSEAGTACSQLAAVLPRWSSVEHGNARQLFERYLAAT
                     RHLAEEAATPL"
     gene            17933..18673
                     /locus_tag="PKN55_00395"
     CDS             17933..18673
                     /locus_tag="PKN55_00395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012696266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HNX69732.1"
                     /translation="MSRRPAYPSAEPANAGVPDIERHISLVKKIAYLLMSRLPASVEL
                     DDLVQVGLIGLIEASRQFDASQGVLFETFASQRVRGAMLDELRRADWLPRQARKQAKQ
                     IEEAISRLEQNLARPPREPEIAEALGLSLDDYQDALFDCKGHQLVYYEDFAVDEDQGG
                     GSVVESIADVRQVDPIDILNDSGFRHGLVEAIKTLPERDQLVMSLYYEQELNLKEIGE
                     VLGVTESRVCQLHSQAVARLRTKLRDWF"
     gene            18810..19631
                     /locus_tag="PKN55_00400"
     CDS             18810..19631
                     /locus_tag="PKN55_00400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018152150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein"
                     /protein_id="HNX69733.1"
                     /translation="MDVISLLGLAVGLASILLGQALEGGHIGSLFQPTALLIVMGGTA
                     GAVMLQCTPAQFMLGLRRLPRVFLPPRHDLHQQLADLVSWSQIARKNGILSMEEFARA
                     QEDNPFLQKGLQLLADGVDPVTLRHALELDINSLEQRERFAARVWESAGGYAPTLGIL
                     GAVLGLIHVMENLAEPSKLGGGIAVAFVATVYGVGLANLVFLPVAHKLKTLLTEQINL
                     MEMQVEGLVAIGSGENPRILENRLQGFLDPSRPAPSVAEKPAVQSSTRHEPGDSA"
     gene            complement(19665..19967)
                     /locus_tag="PKN55_00405"
     CDS             complement(19665..19967)
                     /locus_tag="PKN55_00405"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HNX69734.1"
                     /translation="MFLAMNRFRIARGQEDAFIAIWRERNSYLEQVPGFIHFHLLQGA
                     STEEYTLFASHAEWESEDAFQAWTRSDAFRQAHANAGARRELYLGPPQLELFKSVL"
     gene            20175..22619
                     /locus_tag="PKN55_00410"
     CDS             20175..22619
                     /locus_tag="PKN55_00410"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNX69735.1"
                     /translation="MHTPTPLDSAIMASMAGEASFQHLFMANPQPMWIFDLSTLRFLA
                     VNQAALQHYGYSQAEFLDLTIRDIRPPEDVTRLLDVLSEQASPFNETGLWKHLTRDGR
                     VIDVEVTTHLIQFAGQRAKLVLINDVSERLRAERALREHEARLRLFIEHAPVALAMFD
                     RDMRYLAASRRWQDDYGLGHHPLDGRCHYEVFPEIGARWRELHQRGLQGEALNCEAER
                     FVRQDGRVQWLRWTIHPWRDDQGDVGGIVIFTEDISERKRTEETLRQSATIFESMRDG
                     VLITDLSPAIVAVNRAYTDITGYTLDEVRGLNPSLLRSGRHDHDFYQLMWKNVLEEGH
                     WVGEIWNRRKSGELYPQLLSISTVYDPQGAPCNYVGVFTDISQVRQSEARLEHLAHFD
                     PLTDLPNRLLAYSRLQHAIERGQRTDRRVAALLIDIDRFKNVNDSLGHHIGDQLLQAA
                     AARLRAVAVSVDTVARLGGDEFLLILDNLDDPAPAAALAQTLLDALAEPFMLDEHPPI
                     YLSASIGISLYPDDACDATELVQHADLAMFRAKQARNAYHFHTEAMSQSMREQLLLEN
                     RLRQALDGEEFVLHYQPLIGADGQMAGCEALVRWQPADGPMVPPGQFIPLAEELGLIV
                     PLGGWVLAEACRQGRIWLDAGQPPRVMAVNISARQFQHPDFVNTVRATLAASGFPPAC
                     LELELTESMLMEQVEQSIDTMCALKALGARLSIDDFGTGYSSLAYLKRFPIDKLKIDQ
                     SFVRGIGLERSDGEIITTIIAMARALGLQVLAEGVETAEQQAFLQQHGCQYYQGYLFC
                     RPQPAPALEAWLAKRI"
     gene            complement(22679..23461)
                     /locus_tag="PKN55_00415"
     CDS             complement(22679..23461)
                     /locus_tag="PKN55_00415"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011134441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004324 - ferredoxin-NADP+ reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP reductase"
                     /protein_id="HNX69736.1"
                     /translation="MANANMTAERVLSVHHWNDTLFSFTCTRDEGLRFINGQFVMIGL
                     EVNGKPLMRAYSIVSSNYEEHLEFYSIKVQDGPLTSRLQHLQVGDTVLISKKPTGTLV
                     QDNLLPGKNLYLLSTGTGLAPFMSIIKDPEVYERYEKVILTHGVRWVSELGYHDYITN
                     ELPNNEYFGDIVREKLIYYPTVTREPFRNQGRLTDLIANGKLCADIGLPQLNPEHDRV
                     LICGSPSMLSDLCDMLNGMGFKESPRMGDPADYAIERAFVEK"
     gene            complement(23808..24740)
                     /gene="cysK"
                     /locus_tag="PKN55_00420"
     CDS             complement(23808..24740)
                     /gene="cysK"
                     /locus_tag="PKN55_00420"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012696454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HNX69737.1"
                     /translation="MNIAQNVAALVGNTPLVRLNRLTAGAEATVVAKLEFFNPGHSVK
                     DRIAVAMLDAAEAAGKIGPDTIILEPTSGNTGIGLAMVCAARGYRCTIVMPETMSRER
                     RQLLRAYGAELILTPGPEGMGGAIAKAQALAAADSRYFIPQQFENPANPAIHRATTAE
                     EIWADTDGQVDIFVSGVGTGGTVTGVGEVLKAKKPGVKIVAVEPQASPVLSGGPKGPH
                     PIQGIGAGFVPAILNTSVYDEVIQVSNDNAFETARAMATQEGLLVGISSGAATWAALE
                     LARRPENAGKLIVVVIPSFGERYLSTPLFQHLAD"
     gene            complement(24946..25443)
                     /locus_tag="PKN55_00425"
     CDS             complement(24946..25443)
                     /locus_tag="PKN55_00425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011136522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX69738.1"
                     /translation="MRALLPLTCVILLGGCNWLGNVSGLNREANEAIGAGCRQTGRSL
                     EECFLRHPEADRAQVYAGWRGMNEYMTKNNLTTMAPPADPKPAEQKASAKEGSSSRNR
                     AERYADAESDAGKPDPEVEAVLRNLRSGKPSSISQAQEGELGRVLSIINDPSLAPPTP
                     PGGKQ"
     gene            complement(25449..26204)
                     /locus_tag="PKN55_00430"
     CDS             complement(25449..26204)
                     /locus_tag="PKN55_00430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012697511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNX69739.1"
                     /translation="MHATQYDIPSELLAGRVILVTGATQGIGRAVALGAARHGATVVL
                     LARNIKRLEAVYDEIVSAGSPEPAAIPLDLLTASDEDFNAVAFKIHQELGRLDGIVHC
                     ASHFYALSPLRDQGIEEWMNQFRINTVAPAALTRACLPLLAQSPDASVLAVGESHGLH
                     PGPYWGGFAVSHAGLPHWVKMAASEWDVCPNLRINLLVPGPVQSPQRTRTHPGEAKTE
                     VPPTDSVVPAFLYWLGPQSRGQSGQIIELNPQR"
     gene            complement(26204..27523)
                     /gene="hpnE"
                     /locus_tag="PKN55_00435"
     CDS             complement(26204..27523)
                     /gene="hpnE"
                     /locus_tag="PKN55_00435"
                     /EC_number="1.17.8.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR03467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxysqualene dehydroxylase HpnE"
                     /protein_id="HNX69740.1"
                     /translation="MENLGVRLSARPRVAVVGGGWAGLAAAVTLADWAEVTVFEAGRV
                     LGGRARRLARAPDGVFDNGQHLLLGAYSECLRLMRRVGAAPEALLWRTPQRLDVAGEH
                     SFAMPPWPAPLNQLAGWLGWRGAPWGERWRWLRALLALRAQGFAAPPEQTVADWLRQQ
                     GQSAWLTRTFWEPLTLAALNTPIERAAMACLAAVLRDGVAGPAAHSDMLMPRVDLSAL
                     FPEPAAHWLTAQGHAIRVGYRVKSLFPVQDGVEVDGEHFAAAVLATAPQHVTALLDRK
                     REDAADWLAPINKLDYQPIYTVYLRAHRPEALPQPWLGLVDAPWGQWVFDRQRCTGHA
                     GWLALVISGPGPHQAWSAAELLSRAAAGLNQRFAGLSAQAEGQVIAERRATFSCDAGL
                     ARAAAQGPWPRLALAGDHVAGPYPATLEGAVRSGAHAAYLLKKVLNC"
     gene            complement(27499..28335)
                     /gene="hpnD"
                     /locus_tag="PKN55_00440"
     CDS             complement(27499..28335)
                     /gene="hpnD"
                     /locus_tag="PKN55_00440"
                     /EC_number="2.5.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019921000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016767 - geranylgeranyl-diphosphate
                     geranylgeranyltransferase activity [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="presqualene diphosphate synthase HpnD"
                     /protein_id="HNX69741.1"
                     /translation="MTPDQYCEDKAAKSGSSFYYSFRFLPEDRRRAVTALYAFCREVD
                     DVVDECHEERVARLKLAWWRSEVERLYAGAPEHPVGQALLPHVRALDLPQGQLLEIID
                     GMEMDLDQARYASFRDLQLYCYRVAGVVGQLCAQIFGYQDRQTLKYAHDLGIAFQLTN
                     IIRDVGEDARRGRIYLPVEDLQRFNVPADDILNYRETPAFRELMLFQIERAEQFYTQA
                     LAQLPKVDRKPQRPGLMMAAIYRATLDEIRRDGAEKVLNQRLSLTPIRKLWLAWKTSV
                     FG"
     gene            complement(28512..29327)
                     /gene="hpnC"
                     /locus_tag="PKN55_00445"
     CDS             complement(28512..29327)
                     /gene="hpnC"
                     /locus_tag="PKN55_00445"
                     /EC_number="2.5.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011136526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004311 - farnesyltranstransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene synthase HpnC"
                     /protein_id="HNX69742.1"
                     /translation="MSVGHYENFPVGSLLLPARIRPAVHAIYHFARSADDLADEGDAA
                     PEARLAALDDYRQRLLAIAGGGDGDGHPVFAPLAEQMARHALPAQPFLDLLSAFSQDV
                     SVTRYASFADLIGYCRRSANPVGRLMLHLFNEHDSRSLAMSDGICTALQLTNFWQDVA
                     LDWQKGRVYLPQDALAKYRISEAQIAAGDTGGLWSVMMREQIGRARSMLAAGAPLARQ
                     LPGRFGLELRLIVLGGETILRKLHQSGGDVFRQRPTLQARDWLYMGWRALRKR"
BASE COUNT         5389 a         9047 c         9475 g         5615 t
ORIGIN      
        1 cggctgggaa tggataatta attcaatttc taaatagaaa cggaattagc acatcaacca
       61 gcattatacg tcttccacca acgctgaaag gtatggccca gattttggcg accccagttg
      121 gtttagcgcg atagaaaagc aactgcattt gcccaatagc agtgggtcca cgcaggggca
      181 gcgctgtcag ctgttggacg ccgccttgcg tcggcatatt tttttgcgtc accccctgtt
      241 gggcgtggtg acatggtaag cgccgttccc accccaagtg cccagcttga cgccgccgcc
      301 tgctgcatcc tgtccgccaa gacctgctaa aatcagcttt tgccgcgcct ggcgcgcgct
      361 ctggacgcct gcatgactga ttacatcccc aatcccccgc cgtctgacgc cgagtatcag
      421 cagttgcgca tgccgccgca ttcgctggag gctgagcaat cggtgctggg cggtttgttg
      481 ctggacaatt ccgcctggga ccgcatcggc gatgcggtgg ccgacgaaga tttttaccgc
      541 catgaccacc ggctgatttt tcaggccatc gtcaagctga tcgagcgcag tcacccggcg
      601 gatgtggtga cggtgtccga gcagctggaa aaaagcgccc agctgggcga ggtgggtggg
      661 ctggcgtatc tggccacgct ggcgcaaaac acgccgtcgg cggccaatat caagcgctac
      721 gccgagattg tgcgcgagcg ttcgatcatg cgtcagctgg cgcagattgg caccgagatt
      781 gccgattcgg cgtataaccc gatgggccgc gatgcgcgcg agctgctgga cgaggcggaa
      841 accaaggtgt ttcgcattgc cgagagcacg gcgcgttcgc agcaggattt tctcaatatg
      901 gagcagatcc tcaccgaagt ggtggagcgg gtgaacgagc tgtacggccg cgacaatctg
      961 gacgacgtga ccggcattcc tactggcttt gtcgatctgg acaaaaaaac ctccggcctg
     1021 caaaagggcg atctggtgat tgtggccggc cggccgtcga tgggtaaaac cgcgttttcg
     1081 atgaatattg ccgagaatgt cgcgctgaac accggcttgc cggtggcggt gttttcgatg
     1141 gaaatgggcg ccacccagtt ggcgatgcgg atgattggct cggtgggccg gctggatcag
     1201 cacgcgctgc gcaccggcca gttgtcggac gaagagatgg acaagttcgg catggcggtg
     1261 tcgcgcttgt gggaggcgcc gttgtttatc gacgaaaccc ccggcttgtc tggcctggag
     1321 ctgcgcagcc gcgcgcgccg gctggcgcgc aagatgggcg gccagttggg cttgatcgtg
     1381 attgactatt tgcagctgat gagcggctcc agcgaccgcc gcgaccaaaa ccgcgcttcc
     1441 gagctgggcg agatttcccg ctcgctcaag gcgctggcca aagagctgca agcgccggtg
     1501 attgcgctgt cgcagctgtc gcgctcggtg gagcagcgca ccgacaagcg cccgatgatg
     1561 tcggatttgc gtgaatccgg cgccattgag caagatgctg atttgattgt gtttatgtac
     1621 cgcgacgagt attacaaccc ggattcgcag ttcaagggta tggccgaggc cattatcggc
     1681 aagcagcgta acggccccac tggccgcgtg ccgctgacct ttctggggca ttattcccgc
     1741 tttgaaaacg cctcgccgca acaagtgtcc ggctgggatc aaggagactg accatgagct
     1801 attttgctcg ccatgtgttt ttgtgcacca atcagcgcgc cgagggcgaa agctgttgca
     1861 acgcgctggg cgccagcgag gcgctggacc acgccaaggg ccgcatcaag tccatgggcc
     1921 tggccgggcc gggcaaggtg cgttttcaga aagccggctg tctggagcgc tgcggcgaag
     1981 gcccggtgct ggtggtctac ccggaagcgg tctggtacac ctatgtggac aagaccgata
     2041 ttgacgaaat catcgacgaa cacctggtca acggccgggt ggtggaacgc ctgaagctgt
     2101 ctgaccatgc ttaaactgca acaagagcca atttatctcg acgggccggt gggccagctg
     2161 gaaaccctgg tgattgcccc caaggacggc gcgccgcgcg gcatcgccct gattgcccac
     2221 cccaacccgc tgcaaggcgg cacgaacacc aataaagtgg tgcagaccac ggcgcgcgcg
     2281 ctcagccagc atggctatat ctgctactgc cccaatttgc gcggggtggg caatagcggc
     2341 ggcgagcacg actatggcca cggtgaagtg gacgacgccc aggcggtggt ggactacgcc
     2401 cgcgcccagc atggcgatgt gccgctggtg ctggcgggtt tctcgtttgg cagctatgtg
     2461 gccgcgcaac tgcgccagcg catcgacgcc cgccatttga tcatgctggg cgcggcggtg
     2521 tcgcccaaat acgccatgcc ccatgtgccg gccgacacca ttgtgattca cggcgagcac
     2581 gacgaagtca tcagcctggc gcaggtgctg gattgggcgc gcccgcaaag cctgccggtg
     2641 gtggtgttcc ccggcgttgg gcatttcttt catggcaagc tggtgttgtt gcagcagtat
     2701 gtgcagcggc tggttccgtc gctgtaagcg ggccgcccct tgactctgga tgggccagcg
     2761 ccggcgcggc tgtatcagca ggtgtttcgc cactggcgcg acgccatcgc cagcggccgg
     2821 ctgcccgctg gtgcgcgctt gccggcggtg cgcgagctgg ccgcccgcca caatgtcagc
     2881 ctgaccacgg cgcggcgggt ggtagaagag cttcacgccg gcggctggat tgaaacccgg
     2941 ccgcgtctgg gtttgtttgt ggcccagccg cgtcagcccg gttatccctc gcccgctggc
     3001 cagcccgcgc cggtcagcat cgccaccctg gccttgcggc tgtttgaggc gctgcgcgac
     3061 ccggcgattg cgccgtttgg ctcgccattt atcgacccgg cctggctgga tattgccggc
     3121 gtcaacgacc acttgcgccg cgccgtgcgc aaattctccc ccgcccgtgg cctgaccgac
     3181 ctgccgcccg gccatgatgc cctgcgccag cagattgtcc gccgcgcgct gcatcacggt
     3241 tttcgcttgc agcaagacga attgctgatt acctgcggcg ccatggaggc gctgaatctg
     3301 gcgctgaagt ctgttgcccg ccccggcgcg gtggtggcgg tggaatcccc ggcgttttac
     3361 ggcatgctct acgccatcga ggccaatggc atgcaggcgc tggaaatccg caccgaccca
     3421 cgcaccggcc tggacgtgga cgatctggcg caggcgctgg gccgccaccg cattgccgcc
     3481 tgcatgctga tgccttcgct gcaaaacccg ctgggggcga tcatgccggc ggcggcgcgc
     3541 gcgcgcctgc tggcgctggc tggcgagcac gattttgcca tcatcgaaga cgacgcctac
     3601 cgcgagctgc tgccgcccgg cggcagcccg ccgccgctca agcaccacga cagcgacggc
     3661 cgcgtgctgt attgcggcac ctttgccaag gcgttttcgc cggcgccgcg catcggctgg
     3721 ctggccgccg gccgccacac cgaaaccgcg ctgctgcata aattaatgaa caccctgagc
     3781 acgtcgattc ccaatcaaat ggcagtcgcc agctatctgc gcgatagccg ccccgaccgc
     3841 cggctggcgc ggctgcgcga caccctggcc agcaatcgcg cgcggctggc cgacaccgtg
     3901 cgccggcatt tcccggccgg cagcgaggtg aataccccgg ccggcggatt tttcttgtgg
     3961 gtgacgctgc cgctcgggtg cgacaccttg gcccactatc ccaaggcgct ggcggctggg
     4021 gtgagctttg tgccgggggc gctgttttcg cccagtggcc gttttggtca ctgtttgcgg
     4081 ctaaatgccg gttttgccct ggacgagccg gtgctggcgc ggctggccga agtgggccgc
     4141 caattgcagg ccggttgatt gcgcccggcg gctggccacg gcacacttgc gctttatctg
     4201 aattgacagg agccctcccc catggcgcaa gacagctcgc gtgcagattt ttgggaaacc
     4261 cgctaccagc agggcgtgat tccgtgggat gccgggcaga tgcccagcca gtttgtggcg
     4321 tttgccgctg cccgcccgcc gggcgaacgg gtgctggcgc cgggctgcgg cagcggctac
     4381 gaggtgcgcg gtctggccga acatggcgcc aaggtgctgg cggtggattt cagcgcggcg
     4441 gcggtggcgc gcgcgcgcga ggtgctgggc gagcacgccg actgcgtgcg cgagggcgat
     4501 ttttttcagt tggacgacgc gccgtttgat tggctgtacg agcgcgcgct gttgtgcgcg
     4561 ctgccgccaa aaacctggga cgactgggcg gcgcaaaccg cccgcctggt gcgccccggc
     4621 ggcgtgctgg ccgggtattt ctttattgcc gacacgccca agggcccgcc gtttggcatc
     4681 agcgaggcgc gcttggtcga gctgttgggc gggcatttca ccctggaaag ccgcgagccg
     4741 gccagcgata gcctgccggt gtttgccggg cgcgagcact ggatggtgtg gcgtcggcgc
     4801 tgagggtgcg acaatttgcc gcattgtcgg cgctgcctca cgccggctat catcgccgcc
     4861 tgtgatttgc ggcgttgcgc cgccctggag atgaacgatg aaacgcctgc tcgccctcgc
     4921 cctgtttgcc gctgcttcgc tggcccatgc ccatggttat gcggtgggcg agctgaaaat
     4981 cgaccacccc tgggcgcgcg ccacgccagg cgcagccaag aacggcgcgg cctacctgac
     5041 ggtgagcaat accggcaagc aggccgaccg cctggtggcc gccagtgttg acgtggccga
     5101 ccacgccgag ctgcatacgc atctgaacga caatggcgtg atgaaaatgc gccagatccc
     5161 ggatattccg gttgaagccg gcgccacggt caagctgcag ccgggtggcc tgcatatcat
     5221 gctgatggat ttgaagcagc cgctgaaaga aggcgacaag attccgatga ccctgcgctt
     5281 tgagaaggcc ggcgaagtga aggtggatgt gcatgtggac aaggtgggcg ccatgccggc
     5341 ggctgccggg gcccacgccg gtcactaatc gcgctgctca ccaacccccc cctggccttg
     5401 tggtgtgacc ttgtctgagg tgttgtactg tctgcaggca cacgcctgac tgcctacccg
     5461 gactgcttgg ctgagtgggg tgagccgcac tgtgctgttg gtgagcgccc gtctgaaaat
     5521 attgcgcgcc ccgctgtggg cgcgctattt ttttgtatcc ggctcttgat ttgatatcta
     5581 ctgtcgccat atgccgccag tttggtaaat atattcaatc caggagttgt catgtcaggt
     5641 catggttttc atgtgcacgg cccgcacgat catgctgtcg agcatggtgg gcattcgggg
     5701 gacagttttg catcgcgcat tgcggtgatg acggcggtga tgtccacggt gggcgcgctg
     5761 ttcagttatt ccggcggcgc gacgcaaaac gaggcaatgc tgatgaaaaa cgccgcggcg
     5821 attaaaaaaa ccgaggcggc caatcagtgg aattactatc aggccaagag caataagcaa
     5881 aacctggccg aactggcgct gtcgctgagt caggatgccg ccaacaaaga caaataccag
     5941 aaagacatcg agcgctaccg cgccgagaag gccgagatca aggccgccgc tgaaaagctg
     6001 gaggccgaat cacaagggtt cgagcacaag agcgacgaag ccatgcacct gcatcaccgc
     6061 tgggcgcagg cgatgaccgg cattcaggtg gccatttcgc tggcggcggt gtccttgctg
     6121 acccggcggc gctggctgaa ttacgccacc tatctggccg ccggtgctgg cgtggtgctg
     6181 ggcggtatgg cgctggcgca tctgtaaatt cacaaattca cgcgcaccag gcgtctgggt
     6241 tagctttgtt caatcaccag gctatcgcca gtgacaaaaa actgcccgcc ggccacatag
     6301 tgcaaggtgc gcagcgccgg atcggcaaag gtccagcggc cgtcgcacca ggcctgatca
     6361 tccgcccagg ccgccagcac ttcggccaga aacatatcgt ggcgttcggc catggctggc
     6421 tctggcaaaa ctttgcattc cagccaggcg gcgcagccgt ccagcagcag gccgccgccg
     6481 tgttcagcgc tggcgccatg caggccaaag gcggcgaatt tgtcgatgtc gcggccgctg
     6541 tggctgccca gttgcaacac cgtatgcgcc aattgctggc cgggcagcgc cagcacaaaa
     6601 tggccgctgg cttcaatcag ctggcgggtg tggctgcttt tgtcgatcac caccgccacc
     6661 ttgggcggct gaaaatccac cggcatggcc caggcggcgg ccatgatatt gcgctgatcg
     6721 gcgtgggcgc tgctgatcag ggtaagcggg ccgtggttga gcagtcgata cgcgtgagcc
     6781 agcgggacag aaatgcgcat gatgaggtgt cgggcaaaac tggcattgtg cctttgccgg
     6841 cttgtccggc caagcggggc ggctgggctg atgcgtgcgt gcgacactgt gtgcggcgag
     6901 atgaaaagct gatgaaaaaa accgtggcgc atcatgccat atgagtgaca cactagccgc
     6961 ttgtcctttt ttggtgcagc catggccgct cccgcttcca gtcacggcat gatcgaagca
     7021 ttggcgtcca ccttgctgga tgttcaggcc ttgatcgacg ctgcgcccgc gtcgtcgatg
     7081 ctgcttgatc gtgacgggcg tattctcacc atcaatcgtg ccggcgctgc ccggctgggc
     7141 ctggcgccag cgcaggcgct gggcctgtat ttgtttgatt tgtttccggt ggaagtggcc
     7201 gcctcgcgcc agcgacgctt gcaggcggtg accgccagcc gccagccgca ggtgttcgaa
     7261 gatcaccgcg atggccagca ttaccgtacg tcggcggtgc cggtgatgga ccgcgacggc
     7321 gaggtgtgcc gggtggcgat tttttccgag gacatcaccg cccagcacga ggccgagcgt
     7381 ttgttgcgcg acagcgaggc gcattaccgc tttctggcgg aaaacagcga agacgtcatc
     7441 tggcagctgg accgcgacat gcacatcacc tacatcagcg cggctgatca gcgcttgcgc
     7501 ggggtggcgc gcggggcggt gctggggcaa tcggtgttca gtctgttttc tccccgtggc
     7561 caggcgattc tggcccgtgt ggtggccgag cgccagcagt gtctggagca aggcctgccg
     7621 ctgccgccca ccctgcgttt tgaggcgccg cagcaatgcg ccaatggcga ggaggtgtgg
     7681 gtggaagtgg tgtccacccg catcgacgat gctcatggcg tgctgtgcgg ttatatcggc
     7741 atgacgcgca atgtggccga gcgccgccgc tatcaggcca tgctggaaga cgccaaccgg
     7801 cagttgcgcg aacagctgga taaagtctct gccttgcagg cgcaattgca ggcgcgtgcc
     7861 caccgcgatg cactcaccgg cctgtataac cgccactacc tgcacgaaac cctgccggat
     7921 ttgctggctc aggctcgcca ggctcgccat gggttggcgc tggtgatggt ggatctggat
     7981 cacttcaagc aaatcaacga ccgccacggc catgtagcgg gcgatctggc catccagact
     8041 gccgcccgcc tgctgatcga acgcgcgcct tcgcgcagcc tgtgctgtcg ctttggcggg
     8101 gaagaatttc tggtggcttt gtgcgggctg acgccggcag cggtatgcgc ctgggcagaa
     8161 aactggtgtg ccgagctggc ggactgtgtg ctcagccatc aaggcgtgcg gattgcgctg
     8221 actgcttcgc tgggggtggc ctggcagccg cagcatggcg acagtgaagc gcagttgctg
     8281 gagcgggcag atcaggcgct gtatctggcc aagcggcgtg gacgcaatca agtgtgtgtg
     8341 tgggcggctg acgaggtctg tgcgctgcgc tcctgagccg tatggccggc ctgatgcggt
     8401 gcaggtgcgg gcgtatcagc tacggtgttc agaaccacgg gccaaaggtg gggcgaccgc
     8461 cacgcgccag ccagcccacc ccggcggcca gcagcgccgg caggatcagc tgacaggcca
     8521 gcgccagtgc gctggcgtgg ttgcggtggc tgcgcacacg atagagctgc aagagcaagc
     8581 tggccagcca gaacaccagc gtcatcgccg ccgggcgata ccagtaggca tggcggatat
     8641 cctggctgaa cagcccgcca gccagcacgc ccagatgacc cagcgccaga gccagcgcat
     8701 cgctggccag cacctggcgg gctggcagcg ccagcgggtc ggggcggcac agcagccagc
     8761 cgccggcaaa cacgccttgc cacaaatagg gataaaaaca cggcagccac agcgcgctga
     8821 ccgccagtga ggggcgcaac aacttaaagc cgatggcgag catgaaccag ctggcgatca
     8881 gcatggccag ggccacgcca tcgccgctgc gcagcacccg ccagacaccg cgcgcggcaa
     8941 aggccggcaa cagcagcaga ctgacccaca gcggcgcgtc ggggtgcatg gtgggctcgc
     9001 cgctcagtgc atggccaggg tgcgctgtag cgcagcagac aggagcgcgc ccagattggc
     9061 caggtagtcg gtttccatgt cggtggtgaa gcggtagctg tcggcgctgg ccagcaccaa
     9121 caggccaaac gcctcgccat tgtcgcggcg cagcgccacc tggccaaacg attgcaacac
     9181 cggctcgcgc gggaaccact caataatgtg ttcggcggca aacgggccgg tgtaggggtg
     9241 ctcaaggttg cgggcaatga tggccagacg tggatcctgg cggtgccatt cacccagcga
     9301 ttcggtggcc gttccccaca ggcgcaggcc ggcgtgggtg atgtcgaagt ctttttccag
     9361 cgcactcagt gtgcggtgga tggcgtcggt ggcctcttcg gtgcgcacca gttgcaccga
     9421 cagcgtatgc aggcgggtaa tggtctggtc gttgtgttcg ccaaagcgga tcaactggct
     9481 cattttggcg ccgagctggc ggtttttgtc gcgcagcgcc agcacttggc gctcgaccag
     9541 cggcaccgcg tattccgagc gcgcgtgtgg tacgcgcacc tgggtgagca gctcgctgtg
     9601 ttggtcaaaa aattcggggt gggcttccag gtaggcggcc acttcgctgg cggtcatgga
     9661 catcgggaca gactcctgtc gggacgtttc ttgcgcgcgc cggtcccgcc cacgggccgg
     9721 cggcgtcggc ttgggttaaa gggtgatgct gccttgatac acggtgaccg ccgggccggt
     9781 catgcgcacc ggctggccgg ggccggccca ttcgatgcgc aaatcgccgc cacgggtgtg
     9841 cacggtgacg gcggcgtcca gcaggccacg acggatgccg gccaccacgg ccgcacaggc
     9901 gccagtgccg caggccaggg tttcgccggc gccgcgctcg tacacgcgca gggcgatttc
     9961 ctggcggttg agcacctgca tgaaaccggc gttcacccgt tgcggaaagc gggcgtgctg
    10021 ttcgatctgc gggccgtgcg cggccaccgg cgcagtgttg acgtcgtcca ccacctgcac
    10081 ggcgtgcggg ttgcccatcg acaccacggt gacgcccagt tcggcgccgt ccggcagcgt
    10141 cagcgggtga atcaccgcat cggcgtcggc ttcgaacggg atttccaccg gcaacaggcg
    10201 cggctggccc atgtccaccg tcaccagacc attgtcggtc agcaccgggg tgatgatgcc
    10261 cttggcggtt tccacgcgga tggcgtgctt gtcggtcagg ccctggtcga agacaaaacg
    10321 ggcaaaacag cgcgcgccat tgccgcattg ctcgacctcg ccgccgtcgc tgttgaaaat
    10381 ccggtagcgg aaatcggtgt cgggcagggc gggttcttcc accagcaaca gctggtcaaa
    10441 acccacgccg gtgtgacggt cgcccagctg gcgaatttgc gcggcgctca ggcgcacgcg
    10501 ctggcgcacg ccgtcgatca ccatgaaatc gttgcccagg ccatgcatct tgctgaagtt
    10561 cagaatcatg aaagcggcta tcttgtgcta gaagttactc gggcgcgcgg cgttggcgct
    10621 gtgaacacgc gcggacgaat cataaactat cttgtcatgt ttcggcaggg tgacgctgcc
    10681 tgccgtttga aacgcggcgt atttaccccg tgcttttgcc ggtatgtcag ccggctgggc
    10741 gcctgcgcag atgcacaaag tggtagccca gcccatcgct gtcgcgcacg tggctgtcgc
    10801 gtgaaagtgc ttgccactgg ctggcgtcaa gcgccggaaa gtaggcgtcg ccgtcaatgt
    10861 cggcgtcaat ttcggtgatg aacaattcat cggccagcgc cagcgcctgg gcgtacaact
    10921 gggcgccgcc aatcacgaat acgcgcggcg cggggctgtc cgccgccgcg cacagcgcca
    10981 gcgcgtcggg cagcgagcgg gcatgctcgg cgccggcggt ttgccagtcc gggttgcggc
    11041 tgaccacgat attgcgccgg ccgggcagcg ggcggccaat cgaatcccag gtgcgtcggc
    11101 ccatgatgat gggcgcgccc agggtggtgc gcttgaagtg ctgcaggtct tccggtagat
    11161 gccagggcag ggtgttgtct ttgccaatca cccggttgcg cgccatggcg acgatcaggg
    11221 ccagcgtgct cataccgcca cctcggcgcg aatggccgga tgcgggtcat agccttccag
    11281 ggtgaaatct tcaaatctga aggtgaacag gtcggtgacg gcggggttca gtcgcatcac
    11341 cggcagcggg cgcggctcgc gactcagttg cagctgtgct tgctccaggt ggttgctgta
    11401 caaatgggcg tcgcccaggg tgtgaatgaa ttcaccgggc tgcaactggc acacctgcgc
    11461 caccatcagc gtcagcagcg cgtagctggc gatattgaac ggcacgccca aaaagatatc
    11521 ggcgctgcgc tgatacagct ggcaggagag cttgccgtca gccacataaa actgaaacag
    11581 cgagtggcac ggcggcagcg ccatctggtc caccagcgcc gggttccacg ccgagacgat
    11641 catgcgccgc gaatccggcg tgtcacgcag ctggcgcaat acctggctga tctggtcgat
    11701 atgccggcca tccggcgccg gccagctgcg ccactgatag ccatacaccg ggcccagctc
    11761 gccattggcg tcggcccatt catcccagat cgacacgccg tgggctttca ggtaggcgat
    11821 attggtgtcg ccggccagaa accacagcag ctcatggatg atggatttaa ggtgaacttt
    11881 tttggtggtg accagcggaa aacccttcgc caggtcaaag cgcatctggt ggccaaagat
    11941 cgagcgcgtg ccggtgccgg tgcggtcggt tttgacgtgg ccgtgttcca gcacatggcg
    12001 catgagatcg agataagcct gcatgaaaaa accaaattcc tttatggtga actgatggac
    12061 acaaaagtcc ggtagaatcg ctccgactcc aattttacga taagcccagc gccgagcctc
    12121 gcacaccatg tccgaagaaa gcgatctcga aaaaacagaa gagccctcag agcagaaact
    12181 gcgacaggct cgggaagacg gtcaggttcc ccgatcgcgg gagctggcga cctttgccgt
    12241 caccatgacc ggtgtgggcc tgttggtggt gctgggcaat cgcattgtcg attccatcaa
    12301 caccattatc cgccgcgaat tcacttttga tcagaaaacc ttgctccagg aaggcctgat
    12361 caacgagcat tttcgccagg gcatgttcga catgctgctg gtgctggcgc cgatcctggg
    12421 cgccttgatg gtggccgcca tcggcccgtc gctgttgctg ggtggctgga attttacgct
    12481 gaattcgatc ggcttcaaat tcaacaaact gaatcctgca ccgggcatca aacggatgtt
    12541 ctccgtggct gcactgatgg agggcggcaa ggcagtgctg aaaagcacgc tgatcggtgg
    12601 ggtatccacc tggatcatct ggcgggaaat gggcgagatc atgcaattga tcaccctgcc
    12661 gctggatgaa agcattgccc agaccatggc gttgaccgtg cacaccacgt ttttggtgac
    12721 cagcacgctg ttgttgctgg tgctgatcga cgtaccgttt cagtggtgga acttccgcaa
    12781 gggcctgcgc atgaccaagg aagaggtcaa gcaggaaaac aagaactcgg aaggcagccc
    12841 tgaggtcaag ggccggattc gccagttgca gcgcgaagcc gcacgcaagc ggatgatggg
    12901 ggccgtgccc gaggccagcg tggtggtcac caacccgacc cactacgcgg tggcgatgaa
    12961 gtacgaagac ggcatggggg cacccaaggt gttggccaag ggcaagctac gtaccgccga
    13021 gcgcatcatt gagctggcca aggagcacaa gatcaccatc atgcgcgcac cgccgtttgc
    13081 ccgcgcgctg taccacaata ccgaaatcgg acaggaaatt ccgcatcaat tgtatactgc
    13141 cgccgcgcag attctggctt atgtgttcca actgaaacat tataatgcgc acggcggcat
    13201 ggctccggtc tacccggatg acctgccggt gccgcgcgag cttgacccag aaagcaagac
    13261 cccgcaagat acttcgccaa ccccgaaatc atgattgatg ctggatggat aaactttccc
    13321 aattcctgac acgttacaac accacctcgc tggccggtcc ggtgctgatc atcatgattc
    13381 tcggcatgat gatcctgccc ctgccggcgt ttctgctgga tatcttcttc accttcaata
    13441 tcgcggtgtc catcatcgtg ctgatggtgg ggttgaacac ccgccagccg ctggagttct
    13501 cggcgtttcc ggccgtgctg ctggtgacca cgctgttgcg gctgtcgctg aatgtggcat
    13561 ccagccggat gattctgctc aatggccata acggcgctga cgcagcaggc aaggtgattg
    13621 aagcgttctc gcactttctg gtgggcagca attacgccat cggtattgtg gtgttcatca
    13681 tcctgaccat catcaacttt gtggtgatca ccaagggcgc cgggcggatt gccgaagtga
    13741 gcgcccgctt taccctggac gccatgcccg gcaagcagat ggcgattgac gccgacttga
    13801 atgccggtct gattggcgaa gaagacgcca aaaaacggcg tgcccagatt gccgaagaag
    13861 ccaacttctt tggctcgatg gatggcgcct cgaagtttgt ccgtggcgat gccatcgccg
    13921 gcatcatgat catgctgatc aatattgtcg gcggcctgag cgtgggcctg atccagcacg
    13981 atctgccgct gggcaaggcg ctggaaacct acaccctgct gaccatcggt gatggtctgg
    14041 tggcgcagat tccgggcctg atcatttcta ctgcggccgg tatcgtggtg tcgcgggtgg
    14101 gcactgaaaa agatctgtcc gagcagatgc tggggcagat gttcgccaac tcgcaggtgc
    14161 tgtacattac cgccgccgtc atcgggctga tcggtattat ccccaatatg ccgcacgtgg
    14221 cgtttttgct gattgcttct tcgctggctg gtctggcctg ggcgctggag cgcagcaagc
    14281 agcgtgctgc tgaagccgaa ttcaatgagc cggatctggc cgatgcccag caattgacag
    14341 agacccccct ggccgaagtc ggctggcgtg atgtgcaggc ggtggatcca gtcggtctgg
    14401 aggtcggcta ccggctgatt cctctggtgg atcgtgccca ggatggcgaa ttgttgcgtc
    14461 gggtgcgcgg catccgtaag aaaatcgccc aggagcttgg ctttctggtg ccatcggtgc
    14521 atatccggga taatctggaa atccggccca atgaatatcg gatcatgctc aagggcgtgg
    14581 aagttggcca gggtgaagcc tttgtcggcc agtatctggc cattaatcca ggtcgggtgc
    14641 tgggcaccat ccaggggact cccaccaccg atccggcctt tggtctgccg gcgttgtgga
    14701 ttgaaggcgc cttgcgtgat gaagcccagt cgctgggcta cactgtggtg gatgccagta
    14761 cggtcgttgc cacgcatatc tccaacatcc tgagcaacca tgccgccgag ttgctgggcc
    14821 gcgaagaaac ccagcagttg gtcgatcatg tggccaagga tgcgcccaag ctggtggaag
    14881 atctcattcc caaggtggtg ccgatcaata tcctgcagaa ggttctgcag aacatgctca
    14941 ccgaaggctt gcacatccgc gatattcgca ccatcatcga aaccctggcc gagcataccc
    15001 ctatcaccca ggatgtggac gatctgactt cggctgtgcg tgtcgccttg ggtcggacca
    15061 ttgtttacca gctatatggc aatgccaacg aattgccggt catgactttg gagccagggc
    15121 tggagcaaat cctgatcaac gccgtacaat ccaagggcgg cggcctggag cccggcttgg
    15181 cagaaaacct gctggccagc accgcgcgcg aagccgaacg ggtcgagcag caaggccagg
    15241 tgccagtgtt gctgaccccg gcggccctgc gacctttgct ggccaaattc ctgcgccgcg
    15301 ccatgccgca actggcggtg ctggcgcata atgaaattcc ggacaacaaa accatccgca
    15361 tcatttcggt ggtgggtggg cgagagtaag cgcttatggt agtgaagaag ttttttgggg
    15421 ccaccactcg cgatgccctc aaacaagtcc gcaacgaact cggcgaagac gccctgattc
    15481 tttccaaccg cccggtgggt ggtggtgtgg aaatcatggc agtggccgat gcggatgtca
    15541 gtgcactggc ttcgagcatg aagccaggtg cagctgctgc ggtggctgca ccagtgccta
    15601 cgcctggcaa gggcccggcg ggtgcggctc cggcaacggc aggcagcaaa cccgcttcgt
    15661 cgatgtccag cgcagtggcg cgtacctatg ccatgccggt ggagccactg gatgatccaa
    15721 ccccatcacg cccggccact accgagacgt tcagcactca ggcgcgctcc agcgcaccgg
    15781 agcccgcacc acggcctgtg ccgtcaatca gcatcccgat gtcggcgctg acggccacca
    15841 caccgccgcc gccgccgccg ccgccctctc cgcaatcggt gtatgcccgc tacgaagcac
    15901 ccgctgaaac agcgcccgcc gcatcggata gcagcgaacg ctttaccgaa gattccctgc
    15961 ctgatctggg tgggcaagct ggtgctgcca acagcgatga ggcactggaa aaactggatt
    16021 cgctgtctgg cgaagtgcgc gaaatgcgca ccatgctgca aagccagctg gccagcctgg
    16081 cctgggccaa tatggaagaa caccacccgc gccaggccga gctgttccgc cagatgctgg
    16141 cgctgggtct gtcgccggcc ttgtgtcgcc agctggtggc caagctgccg gacaatctgg
    16201 gcgacgaagc cgcgctcaaa tgggccaagc tcgcgctggt gcacaacctg aaaaccatcg
    16261 acgagcaaga cgaaatcatc gcccgtggcg gcgtgtatgc gctggtgggc cccaccggca
    16321 tcggcaagac caccacggtg gccaagctgg ccgcccgcgc cactctgcgc cacggcaccg
    16381 agcgcattgc cctgatcacc accgacagct accggattgg cgcgcaggat caattgcgcc
    16441 tgtacggcaa gattctgggt gtatcggtgt tttcggtgca aaacgaggca gatttgcacg
    16501 tgaccctggc ggatctggcc gacaagcatc tggtgctgat tgattccacc ggcatgggcc
    16561 agcgcgatgc ccgagtggat gcacaaaccc agatgtacgc ccacaccgcc gccaatggcg
    16621 gccgcccgat ttcccgcgtg ctggtgctgg cggccaatgc cgaaggcgcc acgctggaag
    16681 acgtggtcaa ccgctatctg cctggcggtg gtctggtggg ctgcgtgatg tccaaaatcg
    16741 acgaggcgcc gaccttgggc ggcgccatcg acaccatcat ccgtcaccgt ttgccattgc
    16801 tgtatgtgac caatggccag cgcgtgccgg aagatctcca cctggccaag tccaactacc
    16861 tgattgatcg agccttcaag atggcgcgcg aagcgagccc ctacagcttg aagagcgacg
    16921 aatatcagct gttgacgtcg gcctttgttg gcaccctgaa ctaggacccg ggccatggaa
    16981 gaccaggccg ccagcctgcg ccgtctgacg cctgccgccc aggtggggcg tagctttgcc
    17041 tttttgggag tgtccggctg cggtgtcacc accatggtca gcgaaatggc agccggcctg
    17101 accgctgcgc agcggcgcac cctgattatt gacgcgcacc ctggtaatcc gctggcacag
    17161 cgttttgtcg atgatccagg ggtgacactg gacatggtcg ccagcagtca gggctccttg
    17221 cacgatgccg ttacccaact gccgtttggc gcttcggtga tgaatgtgca tagccgcccg
    17281 tccacgctga tgcacctgcc cgagccgatc gaacaccgat tggtgtccga atatgaggcg
    17341 cttacccgtg atatcgagct ggtgctgatt gatgcgccat cggtaagcac cgatccggcg
    17401 ctggcggcgc tggccgacaa tctggtgctg gtgcttaccc ccgatatcca gacgctgatg
    17461 caggcctacg ccatggtcaa gcgcctggcg ctggaattcg cccgccgccg ttttgatgtg
    17521 ctggtcaacc gcgcgcgtga catgaacgaa gcccaggcga tttttcagcg cctgtcacaa
    17581 gtgaccagtg agtttcttgg tgtgtcattg cgttgggtag ggtttgtgcc ggaagatccg
    17641 atggtccgtc gtgctgtctt gttgcgcacc acgaccctgc atggttttcc gggcagcgag
    17701 gcaggcacag cctgctcgca actggccgct gtcctgccac gctggagttc ggtcgaacat
    17761 ggcaatgccc ggcaactgtt tgaacgctat ctggctgcca ctcgccactt ggctgaggaa
    17821 gccgccaccc ccttatgatg aaccgcattg ctgaccttac ggcgtttctc ccatgtctga
    17881 agccggatac tcctgccctc cgtctgctcg tcctgctgct gcgggggcgc gtatgagccg
    17941 acgcccggcc tatccttccg ctgagccggc caacgccggt gtgccggata tcgagcgcca
    18001 tatttcgctg gtgaaaaaaa tcgcctatct gctgatgagc cggttgccag cgtcggtcga
    18061 gctggacgat ctggttcagg tggggctgat tggcctgatt gaagcttctc gccagtttga
    18121 tgccagtcag ggcgtgctgt ttgagacctt cgccagccag cgcgtgcgcg gcgccatgct
    18181 cgacgagctg cgccgggccg actggttgcc ccgtcaggcg cgcaagcagg ccaagcaaat
    18241 cgaagaagcc atttcccggc tggaacaaaa cctggcgcgc ccgccgcgcg agccggaaat
    18301 tgccgaggca ctgggcctga gtctggatga ctatcaggat gcgctgtttg attgcaaggg
    18361 ccaccaactg gtgtattacg aggactttgc tgtcgatgaa gaccaaggtg gtggcagcgt
    18421 ggtggaaagc attgccgacg ttcgtcaggt ggatccgatc gacatcctca acgacagcgg
    18481 ttttcgtcat ggtctggtcg aggccatcaa gaccctgccc gagcgcgacc aactggtgat
    18541 gagtctgtat tacgaacaag agctcaacct gaaggaaatc ggcgaggtgc tgggtgtgac
    18601 cgagtcgcgc gtctgccagc tgcacagcca ggcggtggcg cggctgcgca ccaagctgcg
    18661 cgactggttc tgagcatttg ccgcccgaca tgggcggttt cttcatcatt tcattacaac
    18721 aacaagatcc ccatgaccac ccgctttttg cctggccggt tatttacccg gcaccttgag
    18781 ttctgtctgg cccagatgga ggtctgcaca tggatgtgat cagcctgctg ggcctggccg
    18841 ttggcctggc ctccattttg ctgggccagg cgctggaagg tggccatatt ggttcgctgt
    18901 ttcagcctac ggccttgctg attgtcatgg gcggcaccgc cggtgcggtg atgctgcaat
    18961 gcacgccggc gcaattcatg ctgggcctgc gccgcttgcc gcgcgtattt ctgccaccgc
    19021 gccacgatct gcaccaacaa ctggcagatc tggtcagctg gagccagatt gcccgcaaga
    19081 acggcatcct gtcgatggaa gaatttgccc gcgctcaaga ggacaacccc tttctgcaaa
    19141 aaggcctgca actcttggcc gacggggtgg acccggtgac cctgcgtcat gcgctggagc
    19201 tggacatcaa ctcgctggag cagcgcgaac gttttgccgc ccgggtgtgg gagtccgctg
    19261 gcggctatgc gccgacgctg ggcattttgg gcgccgtgct cggcctgatt cacgtcatgg
    19321 aaaacctggc cgagccttcc aagctgggcg gcggtattgc tgtggccttt gtcgccaccg
    19381 tgtacggcgt gggtctggcc aacctggtgt ttctgccggt ggcgcacaag ctgaaaaccc
    19441 tgctgaccga acagattaat ctgatggaaa tgcaggtcga aggcctggtg gcaattggca
    19501 gcggcgaaaa tccccgtatc ctggaaaatc gcctgcaagg ctttcttgat cccagccggc
    19561 cggctccctc ggtggccgaa aagcctgccg ttcagtcctc aactcgccat gagccgggcg
    19621 acagcgcctg agctcactgc cccagcctgc ccgatcgggc aggctcacaa taccgacttg
    19681 aacagttcca gctgtggcgg ccccagatac agctcgcggc gcgctccggc attggcgtgt
    19741 gcctggcgga aggcatccga gcgggtccat gcctgaaagg catcttcaga ttcccactcg
    19801 gcatgcgagg cgaacagcgt gtattcctcg gtgctggcgc cttgcagcaa gtgaaagtgg
    19861 ataaaaccag gcacttgctc cagatagctg ttgcgttcac gccagatggc aataaacgcg
    19921 tcttcttgac cccgggcaat gcgaaagcgg ttcatggcga ggaacatgcg gcaactctcc
    19981 tgaaaaaggg catcccgctg gcttgatcat gcgggtagat gtcagtgtac ccgcccgcgg
    20041 ggcaaaccgc gatgagcagg attcgacatg attggtcctt gacattgacc atgccatgat
    20101 caggtttgac cgcacatttg gtggccccgc cctggctggt tatcatcttt ttggtttgtt
    20161 gtctggagca cggcatgcat acgcccacgc cgctggactc cgccatcatg gcctcaatgg
    20221 ccggggaagc gtcgttccag cacctgttca tggccaatcc acagccaatg tggatttttg
    20281 atctgtccac cttgcgcttt ctggctgtca accaggccgc gttgcagcat tatggctata
    20341 gtcaggccga atttctggac ctgaccatcc gcgatatccg cccaccggaa gatgtgaccc
    20401 gcctgttgga cgtattgtct gagcaggcgt ctcccttcaa tgagaccggc ctgtggaagc
    20461 acctgacccg agatggtcgg gtgattgacg tggaggtgac cacccatctg atccagtttg
    20521 ccggccagcg cgccaagctg gtgttgatca acgatgtatc cgagcgcttg cgcgccgagc
    20581 gtgcgctgcg cgagcacgag gcgcgtctgc gtctgtttat tgagcatgcg ccagtggcgc
    20641 tggccatgtt cgaccgcgac atgcgctatc tggctgccag ccgccgctgg caggatgatt
    20701 atggcctggg ccatcacccg ctggatggcc gttgccacta tgaagtgttc ccggaaatcg
    20761 gcgcccgctg gcgcgagctg caccaacgcg ggctgcaggg cgaagcgctc aactgcgaag
    20821 ctgagcgctt tgtgcgtcag gatggtcgtg tgcaatggct gcgctggacc atccatccct
    20881 ggcgcgatga ccagggcgat gtgggcggta ttgtgatttt taccgaggat atttccgagc
    20941 gcaaacgcac cgaagaaacc ttgcgccagt cggccaccat ttttgaatcg atgcgcgatg
    21001 gcgtgctgat caccgacctg agcccggcta tcgttgccgt caaccgcgct tataccgaca
    21061 tcaccggtta cacgctggac gaagtgcgcg ggttgaatcc cagcctgctg cgctcaggtc
    21121 gccatgatca tgatttttat cagctgatgt ggaaaaacgt gctggaagaa ggtcattggg
    21181 tgggggagat ctggaaccgg cgcaaaagcg gcgagttgta cccgcaactg ctgtcgatca
    21241 gcacggtgta cgacccacaa ggcgcgccct gcaattatgt tggggtattc accgatatct
    21301 cgcaggtgcg ccagtccgaa gcccggctgg agcatctggc gcattttgat ccacttaccg
    21361 acttgcccaa ccgcttgctg gcctattcac ggctgcaaca cgccatcgaa cgcggccagc
    21421 gcaccgaccg gcgggtagcc gctttgctga ttgacatcga ccgcttcaaa aacgtcaacg
    21481 atagcctggg ccatcatatt ggcgaccagt tattacaggc tgccgccgcc cgtttgcgtg
    21541 cggtggcggt gagcgtggac accgtggcgc ggctcggtgg cgatgagttt ttgctgattc
    21601 tcgataacct ggacgacccc gcgcccgcgg cggccttggc gcaaaccctg ctggatgcgc
    21661 tggccgagcc tttcatgctg gacgagcacc cgccgattta tctgagcgcc agcatcggca
    21721 tcagcctgta tccagacgat gcttgcgatg ctaccgaact ggtgcagcat gccgatctgg
    21781 ccatgtttcg cgccaagcag gcgcgcaacg cctaccattt tcataccgag gcgatgagcc
    21841 agagcatgcg cgagcaactg ctgctggaaa accgcctgcg ccaggcgctg gatggtgagg
    21901 agtttgtgct gcattaccag ccgctgattg gcgcggatgg ccagatggcc ggctgtgagg
    21961 cgctggtgcg ctggcagccg gctgatggcc cgatggttcc accgggccag ttcatcccac
    22021 tggccgagga gcttggcctg atcgtaccgt tggggggctg ggtgttggcg gaagcctgcc
    22081 ggcagggccg catctggctg gatgctggcc agccaccacg ggtgatggcg gtcaatatct
    22141 ctgcgcgcca gtttcagcat ccggattttg tgaacaccgt gcgcgccacc ctggccgcca
    22201 gcggctttcc gccggcgtgc ctggagctgg agctgaccga gagcatgctg atggagcagg
    22261 tggagcaatc catcgacacc atgtgtgcgc tgaaggcgtt gggcgcgcgc ctgtcaattg
    22321 acgactttgg cacaggctat tcttcactgg cctacctgaa gcgctttccc atcgacaaac
    22381 tgaaaatcga tcagagcttt gtgcgtggca ttggccttga gcgcagtgat ggcgaaatca
    22441 tcaccaccat catcgccatg gcgcgcgcgc tggggctgca agtgctggct gaaggggtgg
    22501 aaaccgccga gcagcaggct tttttgcagc agcatggttg ccagtactat cagggttatc
    22561 tgttttgccg gccgcagcca gcgccggcgc tggaggcctg gctggcaaag cggatatgac
    22621 aacgccgcgc ggccctggat gggcggcgcg gcgtgcaggc aaaaccacac caaaaaactt
    22681 acttctcgac gaaggcgcgc tcaatggcgt agtcggccgg gtcacccatg cgcggggact
    22741 ctttaaagcc catgccgttg agcatgtcgc acaaatcgct gagcatgctc gggctgccgc
    22801 aaatcagcac gcgatcgtgt tccgggttca gctgcggcag gccgatgtcg gcgcacagct
    22861 tgccattggc gatcaggtcg gtcagacggc cctgattgcg gaacggctcg cgggtgacgg
    22921 tcgggtagta aatcagtttc tcacgcacga tgtcgccgaa gtattcattg ttcggcagtt
    22981 cgttggtgat gtaatcgtgg tagcccagct cgctgaccca gcgcacgccg tgagtcagaa
    23041 tcactttttc gtagcgctcg tagacttccg ggtctttgat gatggacatg aacggcgcca
    23101 ggccggtgcc ggtggacagc agatacaggt ttttgcccgg cagcaggttg tcctgcacca
    23161 gggtgccggt gggctttttg ctgatcagca cggtgtcgcc cacttgcaag tgttgcagac
    23221 gcgaagtcag cgggccgtcc tgcaccttga tgctgtagaa ctccagatgc tcttcgtagt
    23281 tggagctgac gatggaatac gcgcgcatca gcggcttgcc attgacctcc aggccaatca
    23341 tcacaaactg accattgatg aaacgcagcc cttcatcacg ggtgcaagtg aagctgaaca
    23401 gagtgtcgtt ccagtgatga acggacagaa cgcgctcggc ggtcatattg gcattggcca
    23461 tggggctatc gctctcgaat aactgaataa taagtaagca ggattttacc gctgccggcg
    23521 gcgtgcgcct accggtattt cgggttttgc tgaacaacat ttgccctgtg tcaaatacgg
    23581 cgcccgcgcc gccgttcagg cagggcgggc agcggcatcc agacggcgcc agggcggcgc
    23641 aaaactgttg ctgcggcgca acatccaagc tgatgcgccg cactagccag gtttagcgtc
    23701 gctcacaaca cccagcgcag ggctcctggc gaggcgcacg accgtagaca gtacggttag
    23761 tacggcacgg tcgggcaaca aggccaggag ggttttgtgc gccgcgctta gtcggccagg
    23821 tgctggaaca gtggggtaga cagataacgc tcaccaaacg acggaatcac caccacaatc
    23881 agcttgccgg cgttttccgg gcggcgggcc agctccagcg cggcccaggt ggcggcgcca
    23941 gaagaaatcc ccaccaggag gccttcttgg gtggccatgg cgcgagcggt ttcgaaggcg
    24001 ttgtcattgc tgacctgaat cacttcgtcg tacacgctgg tgttcagaat ggccgggaca
    24061 aaaccggcgc caatgccttg aatcgggtgc gggcctttcg ggccgccaga cagcaccggc
    24121 gaggcttgcg gctcgacggc gacaatcttc acccccggtt ttttggcttt cagcacttcg
    24181 cccacgccgg tgacggtgcc gccagtgccc acgcccgaga caaaaatatc cacctggcca
    24241 tcggtgtcgg cccagatttc ttcggcggtg gtggcgcggt gaatggccgg gttggccggg
    24301 ttttcaaatt gctgcggaat aaaatagcgc gaatccgctg cggccagcgc ctgggccttg
    24361 gcgatggcgc cgcccattcc ctcggggcca ggggtcagga tcagctcggc gccgtaggcg
    24421 cgcagcagct ggcgacgctc gcggctcatg gtttccggca tcacgatggt gcagcggtag
    24481 ccgcgcgcgg cgcacaccat ggccaggcca atgccggtgt tacccgaggt cggctccaga
    24541 ataatggtgt ccgggccaat cttgccggcg gcttcagcgg catcaagcat ggccacggcg
    24601 atgcgatcct tgacgctgtg gccggggttg aagaactcca gcttggccac cacagtggcc
    24661 tcggcaccag cggtgaggcg attcaggcga accagcgggg tattgccaac cagcgcggca
    24721 acgttctggg cgatgttcat ggtggatatt cctgatggtg agttcaacaa caacgacgct
    24781 gactggttgg gaataataat gcaggcttgt ccaccctgct ggagttcttt cttctataga
    24841 tcagcgggca cagttataac gctgggccag atttgccccg gttaagcata aacagccagc
    24901 gccggaaaac cggggctggc tgcatggggt aaggcgtgcg tgcgtttact gcttgccgcc
    24961 gggcggggta ggcggcgcca gtgacggatc gttgatgatg gacagcacac ggcccaactc
    25021 gccttcctgc gcctgcgaga tgctggacgg cttgcccgag cgcagattgc gcagcacggc
    25081 ttcaacttcc gggtcgggct tgccggcgtc cgactcagcg tcggcatagc gctcggcacg
    25141 gttgcggctg gaactgcctt ccttggcgct ggctttttgt tcggcgggtt tggggtcggc
    25201 cggcggcgcc atggtggtca ggttattctt ggtcatgtac tcgttcatgc cgcgccagcc
    25261 ggcatacacc tgggcacggt cggcttcggg atgacgcaag aaacattctt ccagcgaacg
    25321 gcccgtctgc cggcaaccgg cgccaatggc ttcgttggct tcgcggttca agccagagac
    25381 attgccaagc cagttgcagc cgccaagcag gatgacacac gtcaggggca gtaaagctcg
    25441 catacggttt acctttgcgg gttgagttcg atgatctggc cgctttgccc acggctttgc
    25501 gggcccagcc agtacaggaa ggccggcacc acgctgtcgg ttggcggcac ttcggttttt
    25561 gcttcgcccg gatgggtgcg ggtgcgttgc ggcgactgca ccgggcccgg caccagcagg
    25621 ttgatgcgca ggttcgggca cacgtcccac tcgctggccg ccattttcac ccagtgcggc
    25681 aggccggcgt gcgacacggc aaagccgccc cagtacgggc cagggtgcag gccatgactt
    25741 tcgcccaccg ccagcaccga ggcatcgggc gattgtgcca gtagcggcag gcaggcgcgg
    25801 gtcagcgctg ccggggctac ggtgttgatg cggaactggt tcatccattc ttcaatgccc
    25861 tggtcgcgca agggcgacag cgcataaaaa tgcgaagcgc aatgcacgat gccatccagc
    25921 cggcccagct cctggtgaat cttgaacgcc acggcgttga aatcttcatc gctggcggtg
    25981 agcaagtcca gcggaatcgc cgccggctcg gggctgccag cgctgacgat ttcatcgtac
    26041 accgcttcca gacgcttgat attgcgcgcc agcagcacca cggtggcgcc atggcgggcg
    26101 gcccctagcg ccacggcgcg gccaatgcct tgcgtggcgc cggtgaccag aatcacgcgc
    26161 ccggccaaga gctcggacgg aatgtcatat tgtgttgcgt gcatcagcaa ttcagtactt
    26221 tcttgagcaa atatgcagcg tgcgcgccgc tgcgcaccgc gccttccagc gtggccggat
    26281 acggcccggc cacatgatca ccagccagcg ccagccgggg ccacgggcct tgtgcggcag
    26341 cgcgggccag gccggcgtcg cagctgaagg tggcgcgccg ttcggcaatc acctggcctt
    26401 cggcctgggc gctcaggccg gcaaagcgct ggttcaggcc cgcggcggcg cggctgagca
    26461 attccgccgc cgaccaggct tgatgcggcc ctggcccgct gatcaccagc gccagccagc
    26521 cggcgtggcc ggtgcagcgc tggcggtcaa acacccactg gccccagggg gcgtctacca
    26581 gccccagcca gggctgcggc agcgcctcgg gccgatgtgc gcgcaaatac accgtgtata
    26641 tcggctgata atccagttta ttgatgggcg ccagccagtc ggcggcgtcc tcgcgttttc
    26701 tgtccagcaa ggcggtgaca tgctgtggcg cggtggccag cacggcggcg gcaaagtgct
    26761 cgccatccac ttccacgcca tcctgaacag gaaacagcga ttttaccctg taacccacac
    26821 ggatggcatg gccttgtgca gtcagccagt gcgccgccgg ctcgggaaac agcgccgaca
    26881 ggtccacgcg cggcatcagc atgtcgctgt gcgccgccgg gccggccacg ccatcgcgca
    26941 gcaccgcagc cagacaggcc atcgccgccc gctcgatcgg ggtgttcagc gccgccaggg
    27001 tcagcggctc ccaaaatgtg cgcgtcagcc aggccgactg cccttgctgg cgcagccagt
    27061 cggccacggt ttgctcgggc ggcgcggcaa agccttgcgc gcgcaaggcc aataaggcgc
    27121 gcagccaacg ccagcgctcg ccccagggcg cgccgcgcca gcccagccag ccggccagct
    27181 gattcagcgg cgccggccag ggcggcatgg caaagctgtg ctcgccggcg acgtccagcc
    27241 gctgcggcgt gcgccacagc aatgcttctg gcgcggcgcc cacccgacgc atcaggcgca
    27301 ggcactcgct gtaagcgccc agcagcagat gctggccatt gtcgaacacg ccatccggcg
    27361 cgcgcgccag ccgccgcgcc cgtccgccca gcacgcggcc ggcttcgaac acggtgacct
    27421 cggcccagtc ggccaacgtc accgccgccg ccaggccggc ccagccgccg ccaaccacgg
    27481 cgacgcgcgg gcgggcgctc agccgaacac cgaggttttc cacgccagcc acagcttgcg
    27541 aatcggcgtc agcgacagcc gttggttcag cactttttcg gcgccgtcgc ggcggatttc
    27601 gtccagtgtg gcgcggtaaa tcgccgccat catcaggccg gggcgctgcg gtttgcggtc
    27661 caccttgggc agttgcgcca gcgcctgcgt gtaaaactgc tcggcgcgct cgatctggaa
    27721 cagcatcaac tcacgaaacg ccggggtttc ccggtagttc aaaatatcgt cggccggcac
    27781 attaaagcgc tgcaagtctt ccaccggcag ataaatccgc ccacggcggg catcttcgcc
    27841 aacatcgcgg atgatattgg tcagctgaaa agcaatgccc aggtcgtggg cgtatttcag
    27901 cgtttgtcta tcttgataac caaaaatctg cgcgcacagc tggcccacca cgccggccac
    27961 ccggtagcaa tacagctgca aatcacgaaa actggcgtag cgcgcctggt ccaggtccat
    28021 ttccatgccg tcgatgattt ccagcagctg gccttgcggc aaatccagcg cacgcacatg
    28081 cggcagcaag gcctggccca ccgggtgctc gggcgcgccg gcgtacagcc gctccacctc
    28141 gctgcgccac caggccagct tgagccgcgc cacccgctct tcgtggcatt cgtccaccac
    28201 atcatccact tcgcggcaaa acgcatacaa agcggtcacc gcccggcggc ggtcttctgg
    28261 caaaaagcga aagctgtaat aaaaactcga gccgcttttt gcggccttgt cctcacaata
    28321 ttggtcgggc gtcactgtgt cgggcatcct cgcatggggt tttcaataag aaccgctcac
    28381 aaaaccctcc tggcgttgtt gcgcggcctt gccgtacttg tttgtactgt ctgcggccgc
    28441 acgcctggtc aggcgcgctg cgctgggttt tgtgagcgac gctaaatggg gcttggcgct
    28501 gcggatagca ctcagcgttt gcgcaaagcg cgccagccca tatacaacca atcacgcgcc
    28561 tgcaaagtgg ggcgctggcg gaacacatcg ccgccgcttt ggtgcagctt gcgcaaaatg
    28621 gtctcgccgc ccagcacaat caagcgcaac tccaggccaa agcggccggg caattgtcgg
    28681 gccagcggcg cgccggcggc cagcatgctg cgggcgcggc caatctgctc gcgcatcatc
    28741 accgaccaca gcccgccggt gtcgcccgcg gcgatctgcg cctcgctgat gcggtatttg
    28801 gccagcgcat cctgcggcag gtagacccgg cctttttgcc agtccagcgc cacatcctgc
    28861 cagaaattgg tcagctgcaa ggcggtgcag atgccgtcgg acatcgccag gctgcggctg
    28921 tcgtgttcgt taaacagatg cagcatcagc cgccccaccg gattggccga gcgccggcaa
    28981 tagccgatca aatcggcaaa gctggcgtag cgggtgacgc tgacatcctg gctgaaggcg
    29041 ctgagcaaat ccagaaacgg ctgcgccggc agcgcatggc gcgccatctg ctcggccagt
    29101 ggggcaaaca ccggatggcc gtcgccatcg ccgccgccgg caatcgccag caggcgctgg
    29161 cgatagtcgt ccagcgccgc cagccgcgcc tcgggcgcgg cgtcgccttc gtcggccaga
    29221 tcatcggcgc tgcgggcaaa gtgataaatg gcgtgtaccg ccgggcggat gcgcgccggc
    29281 aacagcagcg aacccaccgg aaagttttcg taatggccaa ccgacatgcg ccatccttgc
    29341 aggaacgaga caatcaaagt ataccctgac tgcgattgtg aaaaattttt cgcccacgct
    29401 ctttacactc gccgcacccc acggcataat gcgccccaca ttgaacaacg gccctgccga
    29461 agttcaactg gcctgtgggg gtatagctca gctgggagag cgcttgcatg gcatgcaaga
    29521 ggtcag
//
LOCUS       DAOPYX010000004        26601 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_1796_length_26601_cov_8.315339, whole genome shotgun sequence.
ACCESSION   DAOPYX010000004 DAOPYX010000000
VERSION     DAOPYX010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 26601)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26601)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26601
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1796_length_26601_cov_8.315339"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1019)
                     /locus_tag="PKN55_00450"
     CDS             complement(<1..1019)
                     /locus_tag="PKN55_00450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNX69743.1"
                     /translation="MSLTADLLIHARWIVTVETDGEVLADHALAIRDGKIVAIVPPEG
                     VAAVSAKETVRLPRHVLMPGLVNLHCHAAMTLLRGYADDLALMDWLQNHIWPAEGKHV
                     SDEFVYDGTLIGMAEMIRSGTTTVNDMYFYNSAVARAGLAAGMRTFVGCSILEFPTNY
                     GLNADDYINKGLKERDEFVGESMVTFTLAPHAPYTVSDATFRKVVTLAEQLDLPIHCH
                     IHETQDEINGSLSEHQVRPLARLAALGLLSPRLVAAHMVHLNDEEIALAAHHGVSVAH
                     NPTSNMKLASGIAPVQKLLAAGVNVGIGTDGAASNNKLDMLADTRMSALLAKVGTQDP
                     TAVPAA"
     gene            complement(1201..2535)
                     /locus_tag="PKN55_00455"
     CDS             complement(1201..2535)
                     /locus_tag="PKN55_00455"
                     /inference="COORDINATES: protein motif:HMM:NF018269.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="di-heme oxidoredictase family protein"
                     /protein_id="HNX69744.1"
                     /translation="MSHSQWVLALALSLGGMAAHAVDGATGEREAFSRPMPGLSEDER
                     GQFTRGRSLFNQSWVAAPARDSEVDGLGPLYNRLACVSCHARNGRGLPPQRLGERMLA
                     MLVRLSVPGQGPHGGPRPHPAYGDQFNEEGIPGVPGEGRVSLRWRPVEHRLVSGERRM
                     LRAPQLRFAELAYGPLGQVLTSPRVGQQLVGMGLLDAVPADALLARAAQAKPDGVRGR
                     VNQVWSVSQSQHTVGRFGHKANMASLREQIAGAFLGDLGITSALHPQENCTSVQTACQ
                     QAPSGGAPELDDSQLDAVTFYLAQLEPPAPRPLPAAELRRGQALFARAGCAVCHLPSL
                     RTAAQAATPRLAGRELAAYTDLLLHDMGPGLADGRPDFAASGREWRTAPLWGVGLTRV
                     ISEGEGYLHDGRARTLEEAVLWHDGEARRARLRFEGFSAADRAALLRFVASR"
     gene            complement(2532..3836)
                     /locus_tag="PKN55_00460"
     CDS             complement(2532..3836)
                     /locus_tag="PKN55_00460"
                     /inference="COORDINATES: protein motif:HMM:NF024921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HNX69745.1"
                     /translation="MSIPPTPARRALLRGGAALALAAWAPWLHAAPRRDITVLTAYPD
                     AVVSRVEAAFERAFPHYRLRMVWRMPHDAGPYLLEPGQHGVDVYWSASPNTYARLKAA
                     GALQALNVDFTGLPARLGGSPLRDADGYYTASETAGYGFVLNPTRLAALNLAAPSDWP
                     ELAAPAYAGQLLLPNPMEVGFAPVLVDIPLQSYGWDTGWALWSAIAANGDFIGRGGGF
                     VSEEIASGRKAIGLSIDFFVVSAIANGAPLRFVYPRQGGINPGHIAITASSPNPVGAR
                     DFVRFVLSADGQALLTHPDIRKLPVRPASYARLPAGHANPFAVADSGGYRYDNGVGQA
                     RAALVAAAFGQACVRPHARLVALWRALRAGDPAQWSKVWPLLVSAPLTEAEASQPALQ
                     QLFANRRDNADSEQQARAHEARWAKQVDARLDQAARLLGVSA"
     gene            complement(3963..6023)
                     /locus_tag="PKN55_00465"
     CDS             complement(3963..6023)
                     /locus_tag="PKN55_00465"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNX69746.1"
                     /translation="MMTPPSLRACLYGCAAASLSLPALAADGGAFELGVVEVIGPRAT
                     PPASKQTVISQSLLRQFNRDTLGEAVGLTPGLSVSRNSRNEDTVYLRGFDIRQVPLFI
                     DGIPAYVPYDGYVDFSRFTTADLAEVRIDKGAASLLYGPNALGGAINLVTRKPTEKLE
                     GDIRAGLAAGNRQQYALNLGSRQDSWYVQLGASYSDSHSFPLSGSYDVRALPTSASNP
                     SRTVLEDGGNRNNAYQTDSRISLKLGFTPNASDEYALGYVRQDGRKGNPPYAGEAPGQ
                     YLSIGGGANSRFWQWPYWDLESAYFISNTALGDAHQLKTRVYHDSYRNKILAYSTGSY
                     STQLSNTSNFPSWYNDSTSGFSVELTSFAINQHVLSLAYHHKIDKHADNGLTLTKRYE
                     DLTRSLALEDRWQLAPAWQLRLGASHDSRSAKEVYYWPTGSTSANNWLAELSHTLHGG
                     AVAYASAARKTRFPTIKDRYSASLGSGLPNAGLTPETALNLELGIKGSPWGGAQGQAA
                     LFHSGIRDLMQRVNITPVSRCNQPGMGAATSCTQWQNIAKARHRGVELQLEQTLGSAW
                     KLGGHYTWLDRDNRSGDTPLTDTPRHKLFAYLNWKPSERWDAQLSVDAEHGRVVAYGS
                     GNAASYTTLPGFALANVKLGYQAMRNVRLEAGVNNLFDANYQLSDGYPMPGRVWFVNG
                     VYQF"
     gene            6668..7891
                     /locus_tag="PKN55_00470"
     CDS             6668..7891
                     /locus_tag="PKN55_00470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008952735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HNX69747.1"
                     /translation="MRAIRPVSLLMLSLLALAGCNKPAEQAAAPAATSGEQVIKLGHA
                     SPMTGPIAHIGKDTEYGAQIAVDELNGEGFTLGGKPVKFQLVSEDDQGDPKMATQIAQ
                     RMVDAKIAGMIGHLNSGTTIPASKIYADANIGQITPSATNPAFTQQGFKTTFRLIADD
                     VQQGKALADFAVNTLKAKKIAIIDDRSAYGQGLADEFDKAAKALGVEIVKREFTSTSA
                     TDFMAILTGIKGAQPDLIFFGGVDAQAAPMVKQMQKLGVKAPLLSGDGTQTGNFIKLA
                     ADAAEGQYASAAGAPKEQLPGYASFAEKLKNKYNAEVVIYSPYSYDAVKVMAMAMKAA
                     DSADPKQYLPKLAATNYQGATGHITFDAQGNRQRSAVTIYQVKQGKWEVIGVVGGENT
                     AQADAPTAGKAAGAQ"
     gene            8088..9314
                     /locus_tag="PKN55_00475"
     CDS             8088..9314
                     /locus_tag="PKN55_00475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012697570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HNX69748.1"
                     /translation="MKYARLSLIATAVLALAACGKGEQKPAEQAAAPAAAPSGELVVK
                     LGQVSPLTGPIAHLGKDSESGVKLAVDELNAEGVTIGGQKVKFELISEDDQADPKIAT
                     QVAQRLVDAKVAAVIGHLNSGTTIPASKVYSDAGIVQISPSATNPAYTTQGFKSAFRV
                     IPNDVQQGKAIGDFIADTLKAKKIAIVDDRTAYGQGLADEVEKAAKGKGVEIVKREFT
                     TNTATDFMAILTSIKGAKPDVIFFGGMDAQAGPMAKQIKKLGITAKLIGGDGFQTPEF
                     IKLAADAAEGQFASNTGVPKEQLPGFATFNEKFKAKYGEVQLYSPYAYDATKLVAAAM
                     KKADSADPKKFLPALAAIEYDGVVGKIKFDDKGDVLNGAITVYQVKGGKWEVMGTVGG
                     AAAPAPAAAPAEEKKQ"
     assembly_gap    9367..9466
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            9483..10172
                     /locus_tag="PKN55_00480"
     CDS             9483..10172
                     /locus_tag="PKN55_00480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018150047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HNX69749.1"
                     /translation="MTTPLLDCVELTTGHGQPRHAVIWLHGLGADGHDFAGIVPELNL
                     PDDLPVRFVFPHAPMIPVTINHGYLLRAWYDILHADGVERTVDQAGIHASRQQIEALI
                     AREAARGVPAERLALVGFSQGGAMAYVTGLSHAQPLAGVAALSTYIPNLADLDALRQP
                     ANHATPVFAAHGEFDNVVPLALGQQAVAALRQRQQPISWRTYPMMHAVCEEEISELGS
                     WLNTVLRAHPA"
     gene            complement(10176..10973)
                     /gene="map"
                     /locus_tag="PKN55_00485"
     CDS             complement(10176..10973)
                     /gene="map"
                     /locus_tag="PKN55_00485"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011135881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HNX69750.1"
                     /translation="MSRSRTVPIRNQEELTQLRRAGALAADVLHMIGEHVKAGVTTNQ
                     LDRLCHDYIVHVQQAVPANVGYHGYPKTICASVNQVICHGIPSDAPLKDGDIVNIDVA
                     VIKDGWYGDSSRMYCVGKPSGLARRLVRTTYDAMRAGILQVRPGATLGDVGYAIQRVA
                     QREGFSVVREYCGHGIGRIYHDEPQVLHYGQPASGLTLKAGMVFTIEPMINAGRPDIR
                     QLADGWTVVTRDNSLSAQWEHMVAVTEHGFEVLTPWPDGQGGYPAID"
     gene            complement(10973..11197)
                     /locus_tag="PKN55_00490"
     CDS             complement(10973..11197)
                     /locus_tag="PKN55_00490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003138839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParD-like family protein"
                     /protein_id="HNX69751.1"
                     /translation="MGIVKISDSMHEALRHSSAALSRSINAQAEHWLRVGMLAELNPT
                     LSYADICQLLIQQAAAAPADENTLTVMRVA"
     gene            11418..12773
                     /locus_tag="PKN55_00495"
     CDS             11418..12773
                     /locus_tag="PKN55_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018749470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrpB-like pyridoxal phosphate-dependent enzyme"
                     /protein_id="HNX69752.1"
                     /translation="MAATRFILPETDIPTHWYNVVADLPSAPAPVLGPDGQPIGPDAL
                     SAIFTDAIIEQEISSERWIAIPEPVRELYQLWRPTPLVRARRLEAALGTPARIYYKYE
                     GSSPAGSHKLNSAIAQAYYNRQAGIRRMTTETGAGQWGSSLAFAGQAFGIDVRVFMVK
                     VSYQQKPFRRSMMQTWGAEVLASPSLHTAAGRAALEADADNPGSLGLAIAEAVEEAAS
                     RKDTTYGLGSVLNHVLLHQTVIGLEAIKQFALAEDYPDMVFAPCGGGSNFGGVAFPFL
                     ADKACGKPVRLVAVEPSSCPTLTRGHYAYDFGDTAGLTPLMKQYTLGHDFMPPSIHAG
                     GLRYHGASSLVSQLYHEGLIEARAVPQLATFEAGVTFARAEGIIPAPESCHAIRAALD
                     EALACKESGESKALFFNLSGHGHFDMASYDKYFAGALEDYDYPEDAIRAALARLPRVG
                     A"
     gene            12860..13660
                     /locus_tag="PKN55_00500"
     CDS             12860..13660
                     /locus_tag="PKN55_00500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX69753.1"
                     /translation="MRFIQYMHPAAQARTDASGLCPANPGPRHQRKFFQVASGHWQAA
                     PHAPVQEGPLAFWGSWEQATRYTPLPASSDKAAPVAAHQPVLSGRARHGKDAVRALPT
                     HPFVFDAPFLFLPGKDSPNRMLSRLEPGDIVVFGSHLHGQFALDTVFVVNGRTPVGDT
                     QVSQLFRRVNDSCFDDTTLPVYRGACLNQPLGALVSFFPAKPATAGEIAAFNRPTLTP
                     VGALEDLVQPRLPHNFRGRETLLPAGAVWDEICRQVMAQGCVLGLSAS"
     gene            14103..14507
                     /locus_tag="PKN55_00505"
     CDS             14103..14507
                     /locus_tag="PKN55_00505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX69754.1"
                     /translation="MLLNLIPAPYRWVAMAIGVALIAAAGAAAASAFYQPRLELARQQ
                     AAEATRAYQALADAAQRQNAAVAQLQSEAEQREQQARQAVSQAQGQAQQWMAQAQAVQ
                     RETAPTSGGHAAACQAASAAFDAELKRERTTS"
     gene            14504..14770
                     /locus_tag="PKN55_00510"
     CDS             14504..14770
                     /locus_tag="PKN55_00510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX69755.1"
                     /translation="MKRSLICALPCALLLAACASTPLPGPVQEVHIPVPVPCKAPDVA
                     RPAFAVDALPLNAGIWRQMQALRSERLQRQGYEIQLEAAVKACQ"
     gene            15013..15810
                     /locus_tag="PKN55_00515"
     CDS             15013..15810
                     /locus_tag="PKN55_00515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011134885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNX69756.1"
                     /translation="MPMHTLADGHSLYYELSGPADADTLVLLNGLTMNTAAWGWHTPL
                     YAGQFQVLTLDLRGQGQSSKPEADAYSLAQQADDVASLLDALGIARAHVLGLSYGGMV
                     AQHLARQHPAKVARLVLASTLAYSDAANAAIAASWEAAQCTGGGDQLFTDMLPWVFGS
                     QFLASQQAMLQAMRALAAQAPWEPVRRLSNGVLAHDARPWLDQLRQPTLVVVGSEDRM
                     TPRYQADALVAGIAGARLEVLPGVGHASHLEALPQFVALTSAFLREA"
     gene            15920..16705
                     /locus_tag="PKN55_00520"
     CDS             15920..16705
                     /locus_tag="PKN55_00520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012696274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HNX69757.1"
                     /translation="MTDSILYSLAANGVATLTLNRPELHNAIDDALIARLSELLDEVQ
                     ANPDVRVLVLTGNGLSFSSGVDQDWMRRMQGYSEAEHLADAQALTRVLHKLDTLRLPT
                     IASVQGSAFGVGAGLVACCDVAVATAEALFGFSEVRLGLIPASTAPYVVRAIGERSAR
                     RYFITAERFNAEKAKRLGLVHIVVEHEALNAQVTYLTETLVRNGPHAIAGSKALLRDV
                     WGKVIDQRLVDFTAERIAHARASDEGREGVAALLEKRKPNWQA"
     gene            17086..>17945
                     /locus_tag="PKN55_00525"
     CDS             17086..>17945
                     /locus_tag="PKN55_00525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX69758.1"
                     /translation="MRTSLSSCLCLLLLTGCATPLTAVRDFANDTRRLSTSFTPMLDH
                     AVQQCEQAFLDRRVYTTDAPLSRFDPAATLAAARSTCQPIAQENAAALALADTLTEYA
                     AQLAALAADGVAQRADDDLDAVAAQAKRFSALPADQVDAVNGLIRFVGHRVLERSQRD
                     AIIEALNHEQAVGALADALARYAERVYRATLNDRLRDQPLLLEQLRQQKAAPIDARLR
                     MLDIQHEIATLQAQQQTIDALHAAVAQLKISLHALRANIDQLSRPELLAELRKLGKEV
                     NTVRKHLATL"
     assembly_gap    17946..18045
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            18099..18497
                     /locus_tag="PKN55_00530"
     CDS             18099..18497
                     /locus_tag="PKN55_00530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX69759.1"
                     /translation="MPDASADQLFDLADQIALISSELLRFRLRQRASLSATDRTRLEA
                     AEVALDQACAQMRAQGITALARQSKAARDEVASTAQAAEAQLRRLKKIERALAIAASA
                     LGLAQAAMAGKPKDITQALGQLKTAISTSV"
     gene            complement(18568..19419)
                     /gene="htpX"
                     /locus_tag="PKN55_00535"
     CDS             complement(18568..19419)
                     /gene="htpX"
                     /locus_tag="PKN55_00535"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019959286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HNX69760.1"
                     /translation="MKRVVLFLATNLAVMLVLSLVASLLGFGRHGGLGSLLGLAMVMG
                     FGGAFISLFMSKMMAKWSTGAEIIDQPRNQTEAWLVDTVARHAQRAGIAMPEVAIYEG
                     EPNAFATGPSRNNSLVAVSTGLLHSMNQEEVEAVIAHEVAHVANGDMVTLTLIQGVVN
                     TFVFFLARVVGSVVDSALRRDGESSGPGIGYMVTVFVCEIAFGILASMIVAYFSRQRE
                     YAADAGAAQLMGNTRPMINALRRLGGADANDLPKDMQAMGIAGGAASLFASHPSLEDR
                     IAALQQQ"
     gene            complement(19960..21396)
                     /gene="cysN"
                     /locus_tag="PKN55_00540"
     CDS             complement(19960..21396)
                     /gene="cysN"
                     /locus_tag="PKN55_00540"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018748389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HNX69761.1"
                     /translation="MSHQSELIASDILGYLKQHENKDLLRFITCGNVDDGKSTLIGRL
                     LHDSKLIFEDHLAAIAKDSQKYNTTDQEIDLALLVDGLQAEREQGITIDVAYRYFSTD
                     QRKFIIADCPGHEQYTRNMATGASTCDLAVILIDARRGVQTQTRRHSFIVSLLGVRHV
                     IVAVNKMDLVDFSQQVFDDIRREYLAFAEGLNIPDIRFVPISALRGDNVVSASERAPW
                     YNGPTLMNLLETVELESDAPLAAFRLPVQYVNRPNLDFRGFCGTIAAGHIRPGDTVQA
                     LPGGKTSKVKAIVTADGELPYAHAGQAVTLTLEDEIDISRGDMLVHVGETAPKVAATL
                     TAHLVWMDETPLTLGKEYLFKLGGKKVTGRVSAIDARIDVNTLEASAASELKLNEIGR
                     VTVALNAAAAFDAYTACRGTGGFIIVDRISNATAAAGMVIDAVADASQHSDADELARL
                     RAFEVELNALVRRYFPHWEAKDIVSKLG"
     gene            complement(21565..22485)
                     /gene="cysD"
                     /locus_tag="PKN55_00545"
     CDS             complement(21565..22485)
                     /gene="cysD"
                     /locus_tag="PKN55_00545"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008954518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0019419 - sulfate reduction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HNX69762.1"
                     /translation="MAIDIPDSRLTHLKQLEAESIHIIREVAAEFDNPVMLYSIGKDS
                     AVMLHLALKAFYPGKPPFPLLHVDTTWKFRDMIAFRDNVVSKYGLKLLTHTNPAGANG
                     EITPFTHGSAKYTDLMKTDALKQALDLHGFDAAFGGARRDEEKSRAKERVYSFRDKNH
                     RWDPKNQRPELWNVYNSQVNKGESIRVFPLSNWTELDIWQYIYLENIDIVPLYFSAPR
                     PVVNRDGLLVMVDDERLPLKEGETPEMRSVRFRTLGCYPLTGAVESTAATLPEIIQEM
                     LLTRTSERQGRAIDHDQSGSMEKKKMEGYF"
     gene            complement(22713..26585)
                     /gene="hrpA"
                     /locus_tag="PKN55_00550"
     CDS             complement(22713..26585)
                     /gene="hrpA"
                     /locus_tag="PKN55_00550"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011135068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HNX69763.1"
                     /translation="MTTPAELKRALDECMARDRHALLSRIRQWEQRRREGKPTDRLAG
                     ELAQAVARSAARAAERRAGLPQPEFDPALPVNQRLDELRAAIERHQVVIVCGETGSGK
                     TTQLPKLCLALGRGVRGLIGHTQPRRLAARSVASRIAQELKSTLGEVVGFKVRFTERS
                     SDKSYIKLMTDGILLAETQTDRYLTAYDTLIIDEAHERSLNIDFLLGFLKQLLPRRPD
                     LKVIITSATIDADRFSQHFDGAPVMEVSGRTFPVEVRYRPLDERDEDQHELDMEAAIV
                     EAVEELARHGPGDILVFLPGEREIRDTAEQLRKARLSAYEILPLFARLSNEDQQKIFR
                     PSGGRRIVLATNVAETSLTVPGIKYVIDTGLARINRYSPRAKVEQLLVEKISQAAARQ
                     RAGRCGRVAAGVCIRLYSEQDFNARPAFTDPEIVRSNLAAVILRMAALRLGKVDEFPF
                     LEAPSSRLVADGYQLLHELGAVSERGEITELGQSLSRIPVDPKVGRMLLAGEQFGCLR
                     EMLILASALSIQDPRERPFDAREAATRAHARFSDEKSDFLSFLALWDFFDEALQNKSS
                     NRQLIQLCHTHFLSHLRMREWRELHAQLSQIADELKLRRNELPATYEPLHKALLTGLL
                     GNVGMKSLEGDDYLGARGIHFHIFPASGLKKARPKWLVSAELTETSKLYARCVASIQP
                     EWLEALAPHLVKYHYFEPHWEKARGEVVASERVTLYGLNIVPRRMVSYGRVAPQEARE
                     IFLREALANGQLSTQAPFYRHNQALIRDIEQLEHKARRQDVLVDEQTLFDFYAERVPA
                     EVCTAAGFDAWRQDAERSNPKLLYLTRDYLMQHAASHITEDQFPPKLSTPDGELTLRY
                     RFEPGHALDGVTVDVPLPLLNRLNPAQLEWLVPGMLREKLALLIKSLPKALRRACVPV
                     PDSVTRFLERQPDGQAALLPQLALFVQRLVGAPVSVADFNPDTLPAHAHMNIRVLDDG
                     KQELGMGRDLLALQRQFGDVAQLTFRDTSLDFERDSVTSWDLGDLPATLKFARNRQQL
                     TGFPALAVEDDRVALRLFDTARTADAAMRGGVIRLLQYALKEQIKQLNKGLPGNFTQT
                     AMLLRNLGHADGLLADAIAAICDRAFIGEDPLPRTEKAFNEQNKRARARLPAVTEAVS
                     RYLADIAAEYQKTLNQAERHKLGPALRAEIGQLVYPGFLAATPWDKLAHLSRYLKAKQ
                     LRMDKYAANPSRDGQRGAEIAQLYKQWEAKLAEAEALEGASQGLKDFRWLIEELRVSL
                     FAQELKTPFPVSLKRLQKAWGEIEENRR"
BASE COUNT         4731 a         8509 c         8408 g         4753 t
ORIGIN      
        1 gcggccggca cggcggtggg gtcttgcgtg cccaccttgg ccagcagcgc gctcatgcgc
       61 gtgtcggcca gcatgtccag cttgttgttg gacgccgcgc cgtcggtgcc gatgccgaca
      121 ttcacgccag cggccagcag cttttgcacc ggggcgatgc ccgaggccag cttcatgttc
      181 gaggtcgggt tgtgcgccac cgacacgccg tgatgggcgg ccagggcgat ttcttcgtcg
      241 ttcaggtgca ccatatgcgc cgccaccagg cgcggcgaca gcaggcccag cgcggccagg
      301 cgcgccagcg ggcgcacctg gtgctcgctc aggctgccgt tgatttcgtc ctgggtttcg
      361 tggatatggc agtgaatcgg caaatccagc tgctcggcca gggtgaccac cttgcggaag
      421 gtggcgtcgc tgacggtgta cggcgcgtgc ggggccaggg tgaaggtcac catggattcg
      481 ccgacaaatt cgtcgcgctc tttcaggccc ttgttgatgt aatcatcggc gttcaggccg
      541 taattggtgg ggaattccag aatcgaacag ccgacaaagg tgcgcatgcc ggcggccaga
      601 ccggcgcggg ccacggccga attgtaaaaa tacatatcgt tgaccgtggt cgtgccgctg
      661 cgaatcatct cggccatgcc gatcagcgtg ccatcgtaga caaactcgtc cgacacatgc
      721 ttgccttcgg ccggccagat gtggttttgc agccagtcca tcagcgccag gtcgtcggcg
      781 tagccgcgca gcagggtcat ggcggcgtgg cagtgcaggt tgaccaggcc gggcatcagc
      841 acatggcgcg gcaggcgcac ggtttctttg gccgacacgg cggccacgcc ttccggcggc
      901 acaatggcga caatcttgcc gtcgcggatg gccagcgcgt ggtcggcgag cacttcgcca
      961 tcggtttcaa cggtcacgat ccagcgggcg tgaatcagca ggtctgcggt cagggacatg
     1021 gcggccttcg tcaataaata gggcatgcaa gcgccgcgcg gcgcgcggca aaggcaggat
     1081 tttaccgcaa tcggcccggg gcggcagcgc agcggcatga tgcggtttca tgaaagcatg
     1141 agtgggataa aaaaagcgcg ccgggggtgg cgcgccaaac agggtgcaac aggagccggc
     1201 tcagcggctg gcgacaaagc gcagcagggc ggcgcggtcg gcagcgctga agccttcaaa
     1261 gcgcaggcgg gcgcggcgcg cttcgccgtc gtgccacagc acggcttctt ccagcgtgcg
     1321 ggcgcggccg tcgtgcagat agccttcgcc ctcgctgatg acgcgggtga ggcccacgcc
     1381 ccacagcggc gcagtgcgcc attcgcggcc gctggcggca aaatccgggc ggccatcagc
     1441 caggccgggg cccatatcgt gcaacagcaa gtcggtgtag gcggccagtt cgcgcccggc
     1501 caggcgtgga gtggcggctt gggcggcagt gcgcaggctg ggcagatggc acaccgcgca
     1561 gccagcgcgg gcaaacagcg cctggccgcg ccgcagctcg gccgccggca gcggacgcgg
     1621 cgctggcggt tccagctggg ccagataaaa agtcaccgcg tccagctggc tgtcgtccag
     1681 ctcgggcgcg ccgccgctgg gcgcctgctg gcaggcggtt tgcaccgagg tgcagttttc
     1741 ttgtgggtgc agtgctgagg tgatgcccag atctcccaaa aatgcgccgg cgatttgctc
     1801 gcgcagactg gccatattgg ccttgtggcc aaaacgtccg acggtgtgct gcgactggct
     1861 gacgctccag acttgattca cccggccgcg cacgccatcc ggcttggcct gggccgcgcg
     1921 cgccagcagg gcgtcggctg gcacggcgtc gagcaggccc atgccgacca actgctgacc
     1981 cacgcgcggc gaggtcagca cctggcccag cgggccgtag gccagctcgg caaagcgcag
     2041 ttgtggcgcg cgcagcatgc ggcgttcgcc gctgaccagc cgatgctcga ccggccgcca
     2101 gcgcaggctg acccgccctt cgccgggcac gccggggatg ccttcttcgt taaattgatc
     2161 gccatacgcc ggatgcgggc gtggcccgcc atgcggcccc tggccgggca ctgacagccg
     2221 caccagcatg gccagcatgc gctcgcccag ccgctgcggc ggcaggccac ggccattgcg
     2281 ggcatggcag gacacgcagg ccagccggtt gtacagcggc cccaggccat ctacttcgct
     2341 gtcgcgcgct ggcgccgcca cccagctttg gttgaacagg ctgcgtccac gggtgaattg
     2401 cccgcgctcg tcctcgctca gccccggcat cggccgggaa aacgcctcac gctcacccgt
     2461 tgcgccatcc acggcatgtg ctgccatgcc gcccaggctt agcgccaggg ctaaaaccca
     2521 ttggctgtga ctcatgcgct gacccccagc agacgcgccg cctgatccag ccgggcgtct
     2581 acttgtttgg cccagcgcgc ctcgtgggcg cgggcttgct gctcgctgtc ggcattgtcg
     2641 cggcggttgg cgaacagctg ttgcagcgcc ggctggctgg cttctgcctc ggtcaatggc
     2701 gcactcacca gcagcggcca caccttgctc cactgtgccg ggtcgcctgc acgcaaggcg
     2761 cgccatagcg ccaccaagcg ggcgtgcggg cgcacgcagg cctggccaaa cgctgccgcc
     2821 accagcgcgg cgcgcgcttg accgacgcca ttgtcgtagc gataaccgcc gctgtcggcc
     2881 acggcaaacg ggttggcgtg gccggcgggc agccgcgcat agctggccgg gcgcaccggc
     2941 agcttgcgga tgtccgggtg ggtcagcagc gcctggccgt cggccgacag cacaaagcgg
     3001 acaaaatcgc gcgccccgac cgggttgggg ctgctggcgg tgatggcgat atggccaggg
     3061 ttgatgccgc cctggcgcgg gtaaacaaaa cgcaagggcg cgccattggc gatggcggag
     3121 acgacaaaaa agtcgatgga caggccaatg gccttgcgcc cgctggcgat ttcttccgag
     3181 acaaaaccgc cgccgcgacc gatgaaatcg ccattggcgg cgatggcgct ccacaaggcc
     3241 cagccggtgt cccagccgta agattgcaat ggaatatcca ccagcaccgg ggcaaagccc
     3301 acctccatgg ggttgggcag caagagctgg ccggcgtagg ccggcgcggc cagctctggc
     3361 cagtcgctgg gcgcagccag attcagcgcg gccagccggg tcgggttgag cacaaagccg
     3421 taaccggcgg tttcgctggc ggtgtagtag ccgtccgcgt cgcgcagcgg gctgccgccc
     3481 agccgcgccg gcaggccggt gaaatccacg ttcagggctt gcagtgcgcc ggctgccttc
     3541 agtcgggcgt aggtgttggg cgaggcgctc cagtacacgt ccacgccatg ctggccgggc
     3601 tccagcagat aggggccggc gtcgtgcggc atgcgccaga ccatgcgcag ccggtagtgc
     3661 ggaaacgccc gctcgaaagc cgcttccaca cgcgacacca cagcatccgg gtaggcggtc
     3721 agcacggtga tgtcgcggcg cggcgcggca tgcagccacg gcgcccaggc ggccagcgcc
     3781 agcgcggcgc cgccacgcaa tagggcccga cgggcggggg tgggtggaat ggacatggca
     3841 ttctccagac agattcagtt caggccgcgt ggcgccgggc gtcgctcacc caaccccgcg
     3901 cagcgatcct ggccagacga cagggccagg caagcacgcc gggcagcttg tgtcagcagc
     3961 gcttaaaact gatacacccc gttgacaaac cacacccgcc ccggcatcgg ataaccgtcg
     4021 ctcaactgat aattggcgtc gaacagattg ttcaccccgg cttccagccg cacattgcgc
     4081 atggcttgat agcccagctt gacgttggcc agggcaaagc ccggcaaggt ggtgtagctg
     4141 gcggcgttgc cgctgccgta ggccaccacc cggccatgct cggcatccac cgacagctgg
     4201 gcatcccagc gctcgctggg tttccagttc aggtaggcaa acagcttgtg gcgcggagtg
     4261 tcggtcagtg gggtatcgcc gctgcggttg tcgcggtcca gccaggtgta atggccgccc
     4321 agcttccagg cactgcccag ggtttgctcc agctgcaatt cgacgccgcg atggcgcgcc
     4381 ttggcgatgt tttgccattg tgtgcagctg gtggcggcgc ccatgccggg ctggttgcag
     4441 cggctgaccg gggtgatgtt gacgcgctgc atcaagtcgc ggatgccgct gtgaaacagc
     4501 gccgcctgac cctgcgcgcc accccagggc gagcccttga tgcccagctc caggttcagc
     4561 gcggtttctg gcgtcaggcc ggcattgggc aggccgctgc ccaaggaggc ggagtaacgg
     4621 tctttgatgg tgggaaagcg ggtcttgcgc gcggcgctgg cgtaggccac cgcgccgccg
     4681 tgcagggtgt ggctgagctc ggccagccag ttgttggcgg atgtgctgcc ggtgggccag
     4741 taatacacct ctttggcgct gcggctgtcg tggctggcgc ccaggcgcag ttgccaggcc
     4801 ggcgccagct gccagcggtc ttccagcgcc agcgagcggg tcaggtcttc atagcgcttg
     4861 gtcagcgtca ggccgttgtc ggcgtgttta tcgatcttgt ggtgataggc caggctcagc
     4921 acgtgctggt tgatggcaaa gctggtcagt tcgacagaaa acccactggt gctgtcgttg
     4981 taccagctgg gaaagttgct ggtgttgctc agttgcgtgc tgtagctgcc ggtgctgtag
     5041 gccagaatct tgttgcggta gctgtcgtgg taaacgcggg ttttcagctg atgggcgtcg
     5101 cccagcgcgg tgttgctgat gaagtaggcg ctttccagat cccagtacgg ccattgccag
     5161 aagcggctgt tggcgccgcc gccaatgctc aggtactggc cgggcgcttc gcctgcgtaa
     5221 ggtgggttgc ctttgcggcc gtcctggcgc acatagccca gcgcgtattc atcgctggcg
     5281 ttgggggtaa aaccgagctt gagcgaaatg cggctgtcgg tctggtaggc gttgttgcgg
     5341 ttgccgccgt cttccagcac ggtgcggctg gggttgctgg cgctggtggg cagcgcgcgc
     5401 acatcgtagc tgccagacag cggaaaacta tggctgtcgc tatagctggc gcccagttgc
     5461 acataccagc tgtcctgccg gctgcccagg ttcagcgcgt attgctgacg gttgcccgcc
     5521 gccaagccgg cgcggatatc gccttccagt ttttctgtgg gtttgcgcgt caccagattg
     5581 atggccccgc ccagcgcgtt ggggccatac agcagcgagg cggcgccttt gtcgatgcgc
     5641 acttccgcca ggtcggcagt ggtaaagcgg gaaaaatcca catagccgtc gtagggcaca
     5701 taagccggaa tgccatcgat gaacagcgga acctggcgga tatcaaagcc gcgcaggtag
     5761 acggtgtctt cgttgcggct gttgcgcgac acactcaggc cgggcgtcag cccaactgcc
     5821 tcgcccaggg tgtcgcggtt gaactggcgc aacaggctct ggctgatcac ggtttgcttg
     5881 ctggccggcg gcgtggcgcg cgggccaatc acctccacca cgcccagctc aaaagcgccg
     5941 ccatcggcgg ccagggcagg caggctcagg ctggcggcag cgcagccata taaacaggcg
     6001 cgcagggaag ggggcgtcat catgcgggtt ctccgggtat ggttgtatct ttttgtatat
     6061 atcgatggcc gtgtcgaatc gcccggcttg atctggttct gcaagtcatg tgccagcaca
     6121 ttgactcagt attttcccga ttttgtcatg tcgttgtata aatcactaca taatgctgtg
     6181 cgctttgcga cagaacggcc ctgtctgcca tcagcaccgc gcccaaaact ccgcgccgcg
     6241 atcctggtcc agatgcacgc gggccgtgcg agaagtgcgg caggcttggg caagcacgtc
     6301 aggagagttt ggtcagtcac tacgagggag tgcgctcggg ttgccggtgg acagcggtgg
     6361 atatgcgcag ttttgtgttt ttgcgacctg ttgtttcaca aaaacaccaa tcacccggaa
     6421 caaaattgcc aaaaatcgcc gttctgggac aataacggac aagctgtcaa accattcgcg
     6481 ccgcccaggc caccctttcc tgagactacg cgtcgtccac gccatccgcc acggtcggcg
     6541 cggcaggctt gcgccaagag cgccgccatt gcccgcgccc atgtttgaca ttccagattt
     6601 gagcaccgac cttgccgccc ggccttgacg ccgcaggcgg ccgaccgttt tttcaggagc
     6661 acccagaatg cgcgcaatcc gccccgtttc tttgttgatg ttgtccttgc tggcgctggc
     6721 cggctgcaat aagccggccg aacaagccgc cgccccggcc gccaccagcg gcgagcaagt
     6781 gatcaagctg ggtcacgcct cgccgatgac cggcccgatt gcccatatcg gcaaagacac
     6841 cgaatacggc gcccagatcg ccgtcgatga actcaatggc gaaggcttca ccctgggcgg
     6901 caagccggtg aaattccagc tggtgtcgga agacgaccag ggcgacccga aaatggccac
     6961 gcaaattgcc cagcgcatgg tggacgccaa aattgccggc atgattggcc acctgaattc
     7021 cggcaccacc attccggcct cgaaaatcta tgccgacgcc aatattggcc aaatcactcc
     7081 gtcggccacc aacccggcat ttacccagca aggttttaaa accactttcc gcctgattgc
     7141 cgatgatgtg cagcaaggca aagcgctggc agattttgcc gtgaataccc tgaaggccaa
     7201 gaaaatcgcc atcatcgacg accgctcagc ctatggtcag ggcctggccg atgaattcga
     7261 caaggctgcc aaggcattgg gtgttgaaat tgttaaacgc gaattcactt ccaccagcgc
     7321 caccgacttc atggccatcc tgaccggcat caagggcgcg cagccggatc tgatcttctt
     7381 tggcggggtg gacgcgcaag ccgcgccgat ggtcaagcag atgcaaaagc tgggcgtgaa
     7441 agccccgctg ctcagcggcg acggcacgca aaccggcaac ttcatcaagc tggcggcaga
     7501 cgccgccgaa ggccagtacg cctcggccgc cggcgcaccc aaagagcaac tgccgggcta
     7561 cgccagcttt gccgagaagc tgaaaaacaa atacaacgcc gaagtggtga tttattcgcc
     7621 gtacagctac gacgcggtga aggtgatggc catggccatg aaagccgccg attcggccga
     7681 ccccaagcaa tacctgccca agctggccgc caccaattac cagggcgcca ccggccatat
     7741 cacctttgac gcccagggca atcgccagcg ctcggcagtg accatttacc aggtcaagca
     7801 gggtaaatgg gaagtgatcg gcgtggtggg tggtgaaaat actgcacagg ccgatgcacc
     7861 gacagcgggc aaggctgccg gcgcgcaata agcatttgcc ggcctttgcg caccttgacg
     7921 cggcgtccct gtgggcgccg cgttttttgt gcactgcaat ggaacatttc ccgccggata
     7981 aggcttggtg gcaaattcaa acgcccgtca aatcgccgtt gacagtgtga aatcgcatca
     8041 tgaataatcg gttcgccttc caaataattt cacaggggaa cgttttcatg aagtatgcgc
     8101 gtttgagcct tatcgccacc gctgtcctgg cactggccgc ttgcggcaag ggtgaacaaa
     8161 agccggctga acaagccgcc gccccggctg ccgcccccag cggcgaactg gtggtgaagc
     8221 tgggtcaagt ttccccgctg accggtccga tcgcccacct gggcaaggac agcgaatccg
     8281 gcgtcaagct ggctgttgac gaactgaacg ccgaaggcgt gaccatcggt ggccagaagg
     8341 tcaagttcga gctgatctcc gaagacgacc aagccgaccc gaaaatcgcc acccaggtgg
     8401 cccagcgcct ggtggacgcc aaggtggccg ccgtgatcgg ccacctgaac tccggcacca
     8461 ccatcccggc ttccaaggtg tactctgacg ccggcatcgt gcaaatctcg ccgtccgcca
     8521 ccaacccggc ttacaccacc cagggcttca agagcgcctt ccgcgtgatc ccgaacgatg
     8581 tgcagcaagg caaggccatc ggtgatttca tcgccgacac cctgaaagcc aagaaaatcg
     8641 ccatcgttga cgaccgcacc gcttacggcc agggtctggc tgacgaagtg gaaaaagccg
     8701 ccaagggcaa gggcgtggaa atcgtcaagc gcgagttcac caccaacacc gccaccgact
     8761 tcatggccat cctgacctcg atcaagggcg ccaagccgga cgtgatcttc tttggcggca
     8821 tggacgcaca agccggcccg atggccaagc agatcaagaa gctgggcatc accgccaagc
     8881 tgatcggcgg cgacggcttc cagaccccgg aattcatcaa gctggctgct gacgccgctg
     8941 aaggccagtt cgcttccaac accggtgtgc cgaaggaaca actgccgggc tttgccacct
     9001 tcaacgagaa gttcaaggcc aagtacggcg aagtgcagct gtactccccg tacgcttacg
     9061 acgccaccaa gctggtggcc gccgccatga agaaggccga ctcggctgat ccgaagaaat
     9121 tcctgccggc attggccgcc attgagtacg atggcgtggt gggcaagatc aagtttgacg
     9181 acaagggcga cgtgctgaac ggcgccatca ccgtctacca agtcaagggc ggcaagtggg
     9241 aagtcatggg caccgtgggt ggcgcagccg ctccggcccc ggcagcagcc ccggccgaag
     9301 agaagaagca gtaatcttct cgcaggcttg accgcaaaaa cggcatcggc aacgatgccg
     9361 ttttttnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnncacc aggagcccca
     9481 ccatgaccac ccccttgctg gattgcgttg aactcaccac cggccacggc cagccgcgcc
     9541 atgcggtgat ctggctgcat ggcctgggcg ctgacggcca cgactttgcc ggcatcgtcc
     9601 ctgaactcaa tctgccagat gatttgccgg tgcgctttgt gtttccgcac gcgccgatga
     9661 tcccggtgac catcaaccac ggctatctgc tgcgcgcctg gtacgacatc ctgcacgccg
     9721 atggtgtcga gcgcacggtg gatcaggccg gcattcacgc ctcgcgccag cagatcgagg
     9781 cgctgattgc ccgtgaggcg gcgcgcggcg tgccggccga gcggctggcg ctggtggggt
     9841 tttcccaagg cggcgccatg gcctatgtca ccgggctgag ccatgcccag ccgctggccg
     9901 gggtggcggc gctgtccacc tatatcccca acctggctga ccttgacgcg ctgcgccaac
     9961 cggccaacca cgccacgccg gtgtttgccg cgcatggcga gtttgacaat gtggtgccgc
    10021 tggcgctggg ccagcaggcg gtggcggcgc tgcgacaacg ccaacagccg atcagctggc
    10081 gcacttaccc gatgatgcac gcagtgtgcg aggaagaaat cagcgagctg ggcagttggt
    10141 tgaacacggt gttgcgcgct caccccgcct gattctcagt caatcgccgg atacccgccc
    10201 tggccatccg gccagggcgt cagcacttca aagccatgct cggtgaccgc caccatatgc
    10261 tcccactgcg ccgacaggct gttgtcgcgg gtgaccacgg tccagccgtc ggccagttgg
    10321 cggatgtccg ggcggccggc gttgatcatc ggttcgatgg tgaacaccat gccggccttc
    10381 agcgtcagcc cggaggccgg ctggccgtag tgcagcactt gcggttcgtc atggtagatg
    10441 cggccaatgc cgtggccgca gtattcgcgc accacgctaa agccttcgcg ctgcgccacc
    10501 cgctggatgg cgtagccgac atcgcccagg gtggcgccgg ggcgcacttg cagaatgccg
    10561 gcgcgcatgg cgtcgtaggt ggtgcgcacc agccggcgcg ccagcccgct gggtttgccc
    10621 acgcaataca tgcggctgct gtcgccatac cagccatcct tgatcaccgc cacgtcgata
    10681 ttgacgatgt cgccatcctt cagcggcgca tcggacggga tgccgtgaca aatcacctga
    10741 ttgaccgagg cgcagatggt tttggggtaa ccgtgatagc cgacattggc cggcacggcc
    10801 tgctgcacat gcacgatgta atcgtggcac agccggtcca gctggttggt ggtgacgccg
    10861 gctttgacgt gctcgccaat catgtgcagc acatcggcgg ccagcgcgcc ggcgcggcgc
    10921 agctgggtga gttcttcttg attgcgaatc ggcacggtgc gcgaacggct catcacgcca
    10981 cccgcatcac ggtgagggtg ttttcatcgg ccggcgcggc ggcggcttgc tgaatcagca
    11041 actggcagat gtcggcatag ctgagcgtgg ggttgagctc ggccagcatg cccacccgca
    11101 gccagtgttc ggcctgggcg ttgatcgagc ggctgagcgc ggcgctgctg tggcgcagcg
    11161 cttcgtgcat ggaatcggag attttgacaa tgcccatggg gaaagtagaa atatatggtt
    11221 tgtatatgaa tcgtatacta gcatgatcgc gttcaggaaa acagccctgc catgcggacc
    11281 gattccgcct ggcgggcggg caattgcccg gtctggtcac gatggattat gctcacggct
    11341 tgccccatgc cgtgaacgtc cggatatctg gccaggggca ctttacccaa caaccgcact
    11401 cacccgagaa gagcatcatg gccgccaccc gcttcatcct gcccgagact gatattccca
    11461 ctcactggta caacgttgtc gccgacctgc ccagcgcgcc cgcgccggtg ctggggccgg
    11521 atggccagcc catcgggccg gacgcgctgt cggccatctt taccgacgcc atcatcgagc
    11581 aggaaatctc cagcgagcgc tggattgcca ttcccgagcc ggtgcgcgag ctgtatcaac
    11641 tgtggcggcc caccccgctg gtgcgcgccc gccggctgga agccgcgctg ggcaccccgg
    11701 cgcgcattta ttacaaatac gaaggcagca gcccggccgg cagccacaag ctcaattcgg
    11761 ccatcgccca ggcttattac aaccgccaag ccggcatccg ccgcatgacc accgaaaccg
    11821 gcgccggcca gtggggcagc tcgctggcgt ttgccggcca ggcctttggc attgatgtgc
    11881 gggtgttcat ggtcaaggtc agctaccaac aaaagccgtt ccgccgctcg atgatgcaaa
    11941 cctggggcgc cgaagtgctg gccagcccca gcctgcacac cgccgccggc cgcgccgcgc
    12001 tggaggctga cgccgacaac cctggctcgc tgggcctggc gattgccgaa gcagtggaag
    12061 aagccgccag ccgcaaagac accacctatg gcctgggctc ggtgctcaac catgtgctgc
    12121 tgcatcaaac ggtgattggc ctggaggcga tcaagcagtt tgccctggcc gaagactacc
    12181 cggacatggt gtttgccccg tgcggcggcg gctccaactt tggcggcgtg gccttcccct
    12241 tcctggccga caaggcctgc ggcaagccgg tgcggctggt ggcggtggag ccgtccagct
    12301 gcccgacgct gacccgtggc cactatgcgt atgattttgg cgacactgcc gggctgaccc
    12361 cgctgatgaa gcaatacacc ctgggccacg acttcatgcc gccgtcgatt cacgccggag
    12421 gcttgcgcta tcacggcgcc agctcgctgg tcagccagct gtatcacgag ggattgatcg
    12481 aagcgcgcgc cgtgccgcag ctggccacct ttgaggccgg cgtgactttt gcccgcgctg
    12541 aaggcatcat ccctgcgccg gagtcttgcc acgccatccg ggccgcgctg gacgaagcgc
    12601 tggcctgcaa ggaaagcggc gagtccaagg ctttgttctt caacctgtcc gggcatggcc
    12661 acttcgacat ggcgagctac gacaaatact ttgctggcgc gctggaagat tacgactacc
    12721 ccgaagacgc catccgcgcc gcgctggcgc gcctgccgcg cgtgggcgcc tgatgccgcc
    12781 gcgctgaggt cgcgcccccg gacagcccgc tcccgccccc gccggagcgg gttttttgcc
    12841 cacagaggaa gctgaattca tgcgcttcat ccagtacatg cacccggccg cccaagcccg
    12901 cacggacgcc agcggcctgt gccccgccaa ccctggcccg cgccaccagc gcaagttttt
    12961 ccaggtggcc agcggccact ggcaggccgc tccccacgcg ccggtgcaag aaggcccgct
    13021 ggccttctgg ggcagctggg agcaagccac ccgctacacc ccgctgccgg ccagcagcga
    13081 caaagccgcg ccggtggccg cacaccagcc ggtgctgtcc ggccgtgccc gccacggcaa
    13141 ggacgccgtg cgcgcgctgc cgacccatcc ttttgtgttt gacgcgccgt ttttgttttt
    13201 gcccggcaag gacagcccca accgcatgct cagccggctg gagccgggcg atatcgtggt
    13261 gtttggctcg cacctgcatg gccaattcgc cctggacacg gtgtttgtgg tgaatggccg
    13321 cacgccggta ggcgataccc aagtctcgca gctgtttcgc cgggtgaacg acagctgctt
    13381 tgacgacacc accctgccgg tgtatcgcgg cgcctgcctg aaccagccgc tgggcgcgct
    13441 ggtaagtttt tttccggcca aaccggcgac tgctggcgag attgccgcct ttaaccggcc
    13501 cacgctgacg ccggtcggcg cgctggaaga tctggtgcag ccgcgtctgc cgcataattt
    13561 tcgtggtcgg gaaaccctgc tgcccgccgg ggcggtatgg gatgaaatct gccgtcaggt
    13621 gatggcccag ggctgcgtgc tgggcctgag cgccagttag tttggttcac cacaccccgc
    13681 gccgcgcgcc tggccaggcg cgtgacgctg cattccaagg ataggctgaa acagccgccg
    13741 catatacgcc accctgaacc caaggtgttg attttttagg atttgttttt gcctggcatg
    13801 atcttgccgc gtatatcgac acatgccgaa accactgacg cgcctcaaca tgcctcgggt
    13861 gctgaccgcc gctaggctgc tcagcagaac ccgaggcaaa tttttttaca gtgggcatgg
    13921 cacacacttt tgcctttggc gttcctgttc aggcagcact tctgatttag cgctgctcac
    13981 caaaccttac ctggcatggt tgcatgacct tgctgtgcga ctggcacggt ctgcggtcgt
    14041 gcgcctggcc aggcgcgctg cgcggggttt tgtgagcggc acttaacgcc caaggaatag
    14101 tcatgttact caacctgatc cccgccccct atcgctgggt ggccatggcg attggcgtcg
    14161 cgctgattgc cgccgccggc gccgctgccg cttcggcctt ttatcaaccc cggctggaac
    14221 tggcccgcca gcaagccgct gaggccaccc gcgcctatca ggcgctggcc gacgccgccc
    14281 agcgtcaaaa tgcggcggta gcccagctgc aaagcgaggc cgagcaacgc gaacaacaag
    14341 cccgccaggc cgtcagccag gctcagggcc aggcccagca atggatggcc caggcccagg
    14401 ccgtgcaacg tgaaaccgct cccaccagcg gtggccacgc tgctgcctgt caggctgcca
    14461 gcgctgcctt tgacgccgaa ctgaaacggg agcgcaccac atcatgaaac gttctctgat
    14521 ctgcgcgctg ccctgcgccc tgttgctggc cgcctgcgcc agcactccac tgcccggtcc
    14581 ggtgcaggaa gtgcacatcc cggtgccggt gccatgcaaa gccccggatg tggctcgccc
    14641 ggcgtttgcg gtggatgccc tgccactgaa tgccggtatc tggcggcaaa tgcaggcgct
    14701 gcgcagcgaa cgcctgcaac gccagggcta cgaaatccaa ctggaagccg ccgtcaaagc
    14761 ctgccagtaa gcgccgctca ctcaatgccc tggcgtagtg gcgcgacctt gcctgtctgc
    14821 ggtcatgcgc tggtgcgcgg agtgtggtga gcggttcttg gcgctgtgca taaaatccgt
    14881 gatgcccccg gcgctgacag gccgggggca tgccattgcg ctgacatggc ccgcacttgc
    14941 cgttacacta ggccatccca gccagcggcc cttgcgacca cctggctcac tcaccccgca
    15001 ggatttgccg acatgcccat gcacaccctg gccgatggcc attcgctgta ttacgaactc
    15061 agcggccccg ctgacgctga caccctggtc ttgctcaacg gcctgaccat gaacaccgcc
    15121 gcctggggct ggcacacgcc gctgtacgcc ggccagtttc aggtgctgac gctggatttg
    15181 cgcggccagg ggcaaagcag caagcccgag gcggacgcct actcgttggc ccagcaggcc
    15241 gacgatgtgg ccagcctgct ggatgcgctg ggcattgccc gcgcccatgt gctggggctg
    15301 tcttatggcg gcatggtggc gcaacacctg gcgcgccagc atccggccaa ggtggcgcgg
    15361 ctggtgctgg ccagcacgct ggcgtattcc gacgccgcca atgccgccat tgccgccagc
    15421 tgggaagccg cccagtgcac tggcggcggc gaccaattgt tcaccgacat gctgccctgg
    15481 gtgtttggca gccagtttct ggccagccag caagccatgc tgcaagccat gcgcgcgctg
    15541 gccgcgcaag cgccgtggga gccggtgcgc cggctgagca atggcgtgct ggcgcacgac
    15601 gcccgcccct ggctggacca attgcgccaa cccaccttgg tggtggtcgg cagcgaagac
    15661 cgcatgaccc cgcgctatca ggccgacgcg ctggtggccg gcattgccgg cgcccggctg
    15721 gaagtgctgc cgggcgtggg ccacgccagc cacctggaag ccctgccgca gtttgtcgcg
    15781 ctgaccagcg cctttttgcg cgaggcctga taccgcctga tctgacgggg tggcttgtca
    15841 tgaaagcgtc aaccacagcg ccgcccttgt cgttacaatg tttgttaatt tcacccgtcc
    15901 gcatgccgag ttctcaccga tgaccgattc cattctgtat tccttggccg ccaatggcgt
    15961 ggccacgctg acgctgaacc gtccggagct gcacaacgcc atcgacgacg ccctgattgc
    16021 ccgcctgagc gagctgctgg acgaagtgca agccaatccg gacgtgcgcg tgctggtgct
    16081 caccggcaat ggcctgagtt tttcttcggg ggtggatcaa gactggatgc gccgcatgca
    16141 gggttacagc gaggccgagc atctggccga cgcccaggcg ctcacccgcg tgctgcacaa
    16201 gctggacacc ttgcgcctgc ccaccatcgc cagcgtgcaa ggttcggcct ttggcgttgg
    16261 cgccggcctg gtggcctgct gcgatgtggc ggtggccact gccgaagcgc tgtttggctt
    16321 ttcggaagtg cgcctggggc tgattccggc ctccaccgcg ccgtatgtgg tgcgcgccat
    16381 tggcgagcgc tcggcgcgcc gctatttcat caccgccgaa cgcttcaacg ccgaaaaggc
    16441 caagcgcctg gggctggtgc atattgtggt cgagcacgaa gcgctcaacg cccaggtcac
    16501 ctacctgacc gaaaccctgg tgcgcaacgg cccgcacgcc attgccggca gcaaggcgct
    16561 gctgcgcgat gtgtggggca aggtgattga ccagcgactg gtggacttta ccgccgagcg
    16621 cattgcccac gcccgcgcca gcgacgaagg ccgcgaaggc gtggcggcgc tgctggaaaa
    16681 acgcaagccc aactggcagg cctgagtaag agtcgctcac gacactcagc gaagcgatcc
    16741 tggcctggcg caccaccgta gacagtacgg agtacggcaa ggctgcgcaa cgacgccagg
    16801 gggagctcta aatgcccgcc gctgcgcaat ctgtcagacg ccctgcctta ccgtgctcag
    16861 tgagcttggc caagtgcctg tttcgcctca gaccatgctg tggcttacca cgaccggccg
    16921 ccctgccctg catggcggcc ggtcagcttg tgtgattgtg tccattgtgc atttctgcca
    16981 tgcttggtga atatcttcac tccgcccagg cggacacccc ggcccgccag gcctgtccgg
    17041 agcctgcgtg gctggcccat ttttgcgccg ggcacgggaa atcacatgcg cacttcactg
    17101 tcatcctgcc tttgtctgct tttgctgacc ggctgcgcca ccccgctgac ggccgtccgg
    17161 gattttgcca atgacacccg ccggctgagc accagcttta ccccgatgct ggatcacgcc
    17221 gtccagcaat gcgagcaagc ctttctggat cgtcgggtgt ataccaccga tgccccgcta
    17281 tcccgctttg atcccgcggc taccttggct gcagcccgca gcacctgcca gccgattgcc
    17341 caggaaaatg ctgccgccct ggccctggcc gataccctga ccgaatacgc cgcgcaactg
    17401 gccgccttgg cggctgacgg cgtggcacag cgcgccgacg atgatctgga cgccgtcgcg
    17461 gcgcaggcca agcgtttttc cgcgctgcct gcggaccagg tcgatgcggt gaacgggctg
    17521 atcaggtttg tgggccatcg cgtgctggag cgtagtcagc gcgacgccat catcgaggcc
    17581 cttaaccacg aacaggccgt gggtgcgctg gccgatgcgc tggcgcgcta tgccgaacgg
    17641 gtgtatcgcg ccacgctcaa cgatcggctg cgtgatcaac cgctgctgct tgagcaattg
    17701 cgccagcaaa aagccgcgcc catcgacgcc cgtttgcgca tgctggatat ccagcatgag
    17761 atcgctaccc ttcaggccca gcagcaaacc attgatgctt tgcatgccgc cgtggcacag
    17821 ctgaaaatct ccctgcacgc attacgcgcc aatatcgatc aactgtcccg gccagagctg
    17881 ctggccgaac tgcgcaaact gggcaaggaa gtcaacaccg tgcgcaagca ccttgccacc
    17941 ctgagnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    18001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnacaaa aaatcacccc
    18061 ggccggcctc gccggccctt cacccaggag agcccatcat gcccgacgcc tctgccgacc
    18121 agctgtttga cctggctgac cagattgcgc tgatttccag cgaactgctg cgctttcgcc
    18181 tcagacagcg cgccagcctg agcgccaccg accgcacccg gctggaagcc gccgaagtgg
    18241 cgctggacca ggcctgcgcc caaatgcgcg cccagggcat cactgccctg gcccgccaga
    18301 gcaaggcagc ccgcgatgaa gtggccagca ccgcccaggc cgccgaagcc cagctgcgcc
    18361 gactgaaaaa aatcgaacgt gcactggcaa ttgccgccag tgccctgggg ctggcccagg
    18421 ctgccatggc gggcaaaccc aaagacatca cccaggcgct tggccagctc aagaccgcca
    18481 tcagcaccag cgtctgaacc aatgaaaaag cccccaggcc agcagtggca gggggcttga
    18541 gacgtggcgt aaccgcagtg atgggtttta ttgctgttgc agcgcagcga tacggtcttc
    18601 caggctggga tggctggcaa atagcgatgc cgcgccgcca gcaatgccca ttgcctgcat
    18661 atccttgggc aaatcattgg catccgcacc acccagacgg cgcaaggcat tgatcattgg
    18721 gcgagtgttg cccatcaact gggccgcgcc ggcatcggct gcatattcgc gctggcgcga
    18781 gaagtaagcc acgatcatgc tggccaggat gccaaaggcg atttcacaaa caaacaccgt
    18841 caccatatac ccgataccgg ggccagacga ctcaccatcg cggcgcaaag cactgtccac
    18901 caccgaaccc accacccgcg ccaggaagaa cacaaaagtg ttcaccaccc cctgaatcag
    18961 cgtcagcgtc accatatcgc cattggccac atgcgccacc tcgtgggcaa tcaccgcctc
    19021 gacttcttcc tgattcatgc tgtgcagcaa gcccgtggat accgccacca gcgaattatt
    19081 gcgcgatggc cccgtggcaa aggcattggg ctcgccctca taaatggcca cctccggcat
    19141 ggcaatgcca gcacgctggg catggcgggc aacggtgtcc accagccagg cttcggtctg
    19201 gttgcgcggc tggtcgatga tctcggcgcc agtagaccac ttggccatca ttttcgacat
    19261 gaacagcgaa ataaaagcgc cgccaaagcc catcaccatg gccagcccca gcaaagagcc
    19321 caggccgcca tggcggccaa agcccagcag gctggccacc aggctgagca ccagcattac
    19381 cgccaggttg gtggcaagaa ataacacaac acgtttgatg ttcatcgcag gcaatccggt
    19441 cagggttgtc aattcacggc agacggcccg catggagaaa agttcgtcaa catgcaggcg
    19501 cagcaaagcg caggagacga gctcccgcct tttgtatatg gatgtgcagg gctgggattt
    19561 caacagggaa aatgacgatg ggcatcaggt gaacgggaga gatgtccagt ttgagcggct
    19621 aacaaaacgc agcgtagtgc tcctggtcgc caatcacgtt gaaacacaaa ggttcaggta
    19681 agcatgtgaa ttcgagtttt aacgccaatg atctatttac atcatggtga ctgactactg
    19741 ggcgcacaac cacacggcaa ggccgaacaa caacgccagg agggttttgt tgatttgtag
    19801 ccgcagggtt aactactagg cataaaaacg ccacgcccct ctcggcacag cggtgcctgg
    19861 aggggcgtgg cgttcattca cacattttgc actacagctc cagtaatacc ccattactga
    19921 gagtctggcg gtgcataaac ggctattttt agcgcattct cagcccaact tgctgacaat
    19981 atccttcgct tcccaatgcg ggaagtagcg gcgcaccagg gcgttgagct ccacttcaaa
    20041 ggcacgcagg cgggccagtt cgtcggcgtc gctgtgctgg ctggcgtcgg ccaccgcgtc
    20101 gatcaccatg ccggcggcgg cggtggcgtt gctgatgcgg tccacgatga taaagccgcc
    20161 ggtgccacgg caggcggtgt aggcgtcaaa ggcggcggcg gcgttcagcg ccacggtgac
    20221 gcggccgatt tcgttcagct tgagctcgct ggcggcgctg gcttccaggg tgttgacgtc
    20281 gatgcgcgcg tcgatggcgc tgacccggcc ggtgactttt ttgccgccca gcttgaacag
    20341 atattccttg cccagggtca gcggggtttc gtccatccac accagatgcg cggtcagcgt
    20401 ggcggccact ttcggcgcgg tttcgcccac gtgcaccagc atatcgccgc gcgagatgtc
    20461 gatttcgtct tccagcgtca gcgtcacggc ctggccggcg tgggcgtagg gcagttcgcc
    20521 atcggcagtg acaatggctt tcaccttgct ggtcttgccg ccgggcaggg cttgcacggt
    20581 gtcgccgggg cggatgtggc cggcggcgat ggtgccgcaa aagccacgga aatccaggtt
    20641 ggggcggttg acgtattgca ccggcaggcg gaaggcggcc agcggggcgt cgctttccag
    20701 ctcgacggtt tccagcaggt tcatcagcgt cgggccgttg taccacggcg cgcgctcgct
    20761 ggcgctgacc acattgtcgc cgcgcagcgc ggaaatcggc acaaagcgga tgtcggggat
    20821 gttcaggcct tcggcaaagg ccagatattc gcggcggatg tcgtcaaaca cctgctggct
    20881 gaagtccacc aggtccattt tgttgacggc gacgatgaca tggcgcacgc ccaagagcga
    20941 aacaataaag ctgtggcggc gggtttgcgt ttgcacgcca cggcgggcgt cgatcaggat
    21001 caccgccagg tcgcaggtgg aggcgccggt ggccatattg cgggtgtatt gctcgtggcc
    21061 cgggcagtcg gcaatgatga atttgcgctg gtcggtgctg aaataacggt aggcgacatc
    21121 aatggtgatg ccttgttcgc gctcggcttg caggccgtcc accagcaggg ccaggtcgat
    21181 ttcctggtcg gtggtgttgt acttttgcga gtccttggcg atggcggcca ggtggtcttc
    21241 aaaaatcagt ttggagtcgt gcagcaggcg gccaatcagc gtggatttgc cgtcatcgac
    21301 gttgccgcag gtgataaagc gcagcaggtc tttgttttcg tgttgcttca aatagccaag
    21361 aatgtcgctg gcaatcagtt cggactggtg ggacatggtc gggttctcta ctgtgtggcc
    21421 gtcgcccgtg tcgggcggcg gcggcaatcc ggtaaatggc gtggcgcacg ctgcgccggg
    21481 cttggggtgc gctgcttggc ggggcaaaac acgggtttgc cacggtcaag ccccgtcggg
    21541 aatcacgggc ggttcagcgc agccttagaa atagccttcc atttttttct tttccatcga
    21601 gcccgactga tcatggtcga tggcgcggcc ctggcgctcg ctggtgcggg tgagcagcat
    21661 ttcctggatg atttccggca gggtggcggc ggtggattcc accgcgccgg tcagcggata
    21721 acagcccagg gtgcggaagc gcaccgagcg catttctggg gtttcgcctt ctttcagcgg
    21781 caaacgctcg tcgtccacca tcaccagcag gccgtcgcgg ttgaccaccg ggcgcggcgc
    21841 cgagaaatac agcggcacga tgtcgatgtt ttccaggtag atatattgcc agatgtccag
    21901 ctcagtccag ttggacagcg ggaacacgcg gatggattcg cccttgttca cctggctgtt
    21961 gtagacattc cacagctccg ggcgctggtt tttcgggtcc cagcggtggt ttttgtcgcg
    22021 gaacgagtac acccgctctt tggcgcggga tttttcttca tcgcggcgtg cgccgccaaa
    22081 agcagcgtca aagccgtgca aatccagcgc ctgcttgagc gcgtcggttt tcatcaggtc
    22141 ggtgtacttg gcgctgccgt gggtgaacgg ggtgatttca ccattggcgc cggccgggtt
    22201 ggtgtgggtc aacagcttca ggccgtattt gctcaccaca ttgtcgcgga aggcgatcat
    22261 gtcgcggaac ttccaggtgg tgtccacgtg cagcagcgga aacggcggct tgcccggata
    22321 aaacgccttg agcgccagat gcagcatcac ggcggaatcc ttgccaatgg aatacagcat
    22381 caccgggtta tcaaactcgg cggccacttc gcggatgata tgaatggatt cggcttcaag
    22441 ctgcttcaga tgcgtcagcc gggagtcagg aatgtcaatc gccatggtgg ttgcagcttt
    22501 ccaagcaagg ttcggtcagg cgggccggca gccggcgcgc ggaagggata cagccagaga
    22561 tgtttggctt tggcaggctg gcactttacc gcaagcgcca gcaagcgcat aaaccaattc
    22621 gccatatcca tatattgaaa ttaacgtgca agccgggtgc ttcctgtggg tttaggcata
    22681 agccacgcac acccggccgg gcaaggcagc gctcagcgcc ggttttcttc gatctcgccc
    22741 caggcttttt gcaggcgttt cagcgacacc gggaacgggg ttttcagctc ctgggcgaat
    22801 aacgacacgc gcagttcttc aatcagccag cggaagtctt tcaggccctg gctggcgcct
    22861 tccagcgctt ccgcctcggc cagtttggct tcccattgtt tgtacagctg ggcgatttcg
    22921 gcgccgcgct ggccgtcgcg gctggggttg gcggcgtatt tgtccatgcg cagctgcttg
    22981 gctttcaggt agcgcgacag atgcgccagc ttgtcccacg gcgtggcggc caaaaagccg
    23041 gggtagacca attggccgat ttcggcgcgc agcgccgggc ccagcttgtg gcgctcggcc
    23101 tggttcaggg ttttttggta ttcggcggcg atgtcggcca gatagcggct gacggcctcg
    23161 gtgacagccg gcaggcgggc gcgggcgcgt ttgttttgtt cgttaaaggc tttttccgtg
    23221 cgcggcagcg ggtcttcgcc aataaaggcg cgatcacaaa tggcggcgat ggcgtcggcc
    23281 agcaggccgt cggcgtggcc caggttgcgc agcagcatgg cggtttgcgt gaaattgccg
    23341 ggcaggcctt tgttcagctg cttgatttgc tctttcagcg cgtattgcaa caggcggatc
    23401 accccgccgc gcatggcggc gtcggcggtg cgggcggtgt cgaacaagcg cagcgccacc
    23461 cggtcgtctt ccaccgccag cgccggaaaa ccggtcagct gctggcggtt gcgggcaaat
    23521 ttcagcgtgg ccggcaggtc gcccaaatcc cagctggtga ccgaatcgcg ctcgaaatcc
    23581 agcgaggtgt cgcggaaggt cagctgggcg acgtcgccaa actggcgttg cagcgccagc
    23641 aagtcacggc ccatgcccag ctcctgcttg ccgtcgtcca gcacgcggat attcatgtgc
    23701 gcatgcgccg gcagggtgtc cgggttgaag tcagccacgc tgaccggcgc accaaccagg
    23761 cgctgcacaa acaaggccag ctgcggcaat agcgccgcct ggccgtccgg ctggcgctcc
    23821 agaaagcgcg tcacgctgtc cggcaccggc acgcaggcgc ggcgcagcgc tttgggcaag
    23881 gatttgatca gcagggcgag tttttcccgc agcatgcccg gcaccagcca ttccagctgc
    23941 gccgggttca gccggttcag cagcggcagc ggcacgtcca ccgtcacccc gtccagcgcg
    24001 tggccgggct caaagcggta acgcagggtc agctcgccgt ccggggtgct cagcttgggc
    24061 ggaaactggt cctcggtgat atggctggcg gcgtgctgca tcaggtaatc gcgggtcaga
    24121 tacagcagct tggggttgct gcgctcggcg tcctgccgcc aggcgtcaaa gccggcggcg
    24181 gtgcacacct cggccggcac gcgctcggcg taaaaatcaa acagggtttg ctcgtccacc
    24241 agcacatcct ggcggcgggc cttgtgttcg agttgttcga tgtcgcgaat cagcgcctga
    24301 ttgtgccggt agaacggcgc ctgggtgctc agttggccat tggccagcgc ctcgcgcaag
    24361 aaaatctcgc gcgcctcctg cggcgccacc cggccatacg acaccatgcg gcgcggcaca
    24421 atattcaggc cgtacagcgt cacccgctcg ctggccacca cctcgccgcg ggctttttcc
    24481 caatgcggct caaagtaatg gtacttgacc agatgcggcg ccagcgcctc cagccattcc
    24541 ggctgaatgc tggccacgca gcgggcgtac agcttgctgg tttcggtcag ctcggccgac
    24601 accagccatt tgggccgggc ttttttcagg ccagaagccg gaaagatatg aaaatgaatg
    24661 ccgcgcgcgc ccagataatc gtcgccttcc agcgatttca tgccgacatt gcccaataag
    24721 ccggtcagca gcgccttgtg cagcggctcg taagtggccg gcagttcatt gcggcgcagc
    24781 ttgagttcat cggcaatctg gcttaactgg gcgtgcagct cgcgccattc gcgcatgcgc
    24841 agatgcgaca aaaaatgcgt gtggcacagc tggatcaact ggcggttgga gcttttgttt
    24901 tgcagggctt cgtcaaaaaa gtcccacagc gccaaaaacg acagaaaatc cgatttctcg
    24961 tcgctaaagc gcgcatgcgc ccgcgtggcc gcctcgcggg cgtcaaacgg ccgctcgcgc
    25021 gggtcttgaa tcgacaaggc cgaggccaga atcagcatct cgcgcaggca gccaaactgc
    25081 tcgccggcca gcagcatgcg gcccactttc gggtcaaccg ggatgcgcga cagcgattgg
    25141 cccagctcgg tgatttcgcc gcgctcgctg accgcgccca gctcgtgcaa gagctgatag
    25201 ccatcggcca ccagccgcga cgacggcgcc tccaaaaacg gaaactcatc caccttgccc
    25261 agccgcagcg ccgccatgcg caaaatcacc gccgccagat tcgagcggac aatttccggg
    25321 tcggtaaacg ccgggcgggc gttgaaatcc tgctcggaat acagccggat gcacaccccg
    25381 gccgccaccc ggccgcaacg gccggcgcgc tggcgcgccg ccgcctggct gattttttcc
    25441 accagcagct gctcgacctt ggcgcgcggg ctgtagcggt tgatgcgcgc caggccggtg
    25501 tcgatcacat acttgatgcc cggcacggtc agcgaggttt ccgccacatt ggtggccagc
    25561 acaatgcgcc gcccgcccga gggccggaaa atcttttgct ggtcttcatt ggacaaacga
    25621 gcaaacagcg gcaggatttc atacgcgctc agccgcgcct tgcgcagctg ctcggcggtg
    25681 tcgcggattt cccgctcgcc gggcaaaaac accagaatat cgccagggcc gtggcgggcc
    25741 agctcttcca ctgcctcgac aattgccgct tccatatcca gctcgtgctg gtcttcgtcg
    25801 cgctcgtcca gcggccggta gcgcacctcc accggaaaag tgcggccaga gacttccatc
    25861 accggcgcgc cgtcaaaatg ctggctgaag cggtcggcgt cgatggtggc cgaggtgatg
    25921 atcactttca ggtcgggccg gcgcggcagc aactgcttga gaaagcccaa caggaaatca
    25981 atattcagcg agcgctcgtg cgcctcgtcg ataatcagcg tgtcgtaagc cgtcagataa
    26041 cggtcggttt gcgtttccgc cagcaaaatg ccgtcggtca tcagcttgat atagcttttg
    26101 tccgagctgc gctcagtaaa gcgcaccttg aagcccacca cctcgcccag cgtggatttc
    26161 agctcctggg caatccgcga cgccaccgaa cgcgccgcca gccggcgcgg ctgggtgtgg
    26221 ccaatcagcc cgcgcacgcc acgccccagc gccaggcaca gcttgggcag ctgcgtggtt
    26281 ttgcccgagc cggtttcccc gcagacaatc accacctgat ggcgctcaat cgccgcgcgc
    26341 agctcgtcca gccgctgatt gaccggcaag gccgggtcga actccggctg cggcagcccg
    26401 gcgcgccgct cggccgcccg cgccgctgaa cgcgccaccg cctgcgccag ctcgcccgcc
    26461 agccggtcgg tcggcttgcc ctcacggcgg cgctgctccc attggcggat gcgggacaac
    26521 aaagcatggc ggtcgcgcgc catacactca tcaagggcgc gcttcagttc ggcgggggtg
    26581 gtcattgcag gcagaaagtg a
//
LOCUS       DAOPYX010000005        24196 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_2088_length_24196_cov_13.070958, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000005 DAOPYX010000000
VERSION     DAOPYX010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 24196)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24196)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24196
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2088_length_24196_cov_13.070958"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            106..505
                     /gene="rnpB"
                     /locus_tag="PKN55_00555"
     ncRNA           106..505
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PKN55_00555"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(570..1034)
                     /locus_tag="PKN55_00560"
     CDS             complement(570..1034)
                     /locus_tag="PKN55_00560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX69764.1"
                     /translation="MKYLVAACLAAVVSGCSAVGYHGGVMTASDGYVLERKITALSGR
                     DVELTVWAHANSEGVLEQTLRTEARAYGVRQGCPNPGIEKLVFGLENAMLDARRYARA
                     TVRCTPSANLAGYNQPGLVNLSPAGALPEPTIDPGMSVKKKTSSKSKKRQSK"
     gene            complement(1056..1604)
                     /locus_tag="PKN55_00565"
     CDS             complement(1056..1604)
                     /locus_tag="PKN55_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019919648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgO family outer membrane protein"
                     /protein_id="HNX69765.1"
                     /translation="MKRLLALTASALCLGLSACATDGSRSEPTYEAAARNELVSTNYR
                     AADVLLGQLRNLDNEGPMLVATLVNIDALERSSTLGRLVSEQVAARFAQQGRAMVELK
                     LRHTMYMRRTEGELALTREIAEVARQHNARAILLGSYGLSGDMVFVNLKVVQPGNNLV
                     LAAYDYALPLNREVRSLLGLPG"
     gene            complement(1604..2530)
                     /locus_tag="PKN55_00570"
     CDS             complement(1604..2530)
                     /locus_tag="PKN55_00570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX69766.1"
                     /translation="MKPTLLRPALPGLALCASLLLSACTTPYSEAPVATNFPTSKQHK
                     LQAGAHWDAIAKDAATTLVTSLRLGKGCIAAYPDCNQLVLRPPREATPFSRAFHTLLT
                     TSLVNQGVQMIPYGSAPQPSMAYTPPPMPAPLVSAPTPRVVKRQKYGKKAYLATAPKP
                     VAQMAPVDAMPVIPPAPAPRQELEVDIQVVKFSPDRLDGRYFVSGTTLGAGVWALQGL
                     WTQTSPQGIATGAALGAAFDAHRWFSSEFARGGVPQLEMIITVSALTNGQYVGRVSNI
                     YYLTDSDLALYMQLPPPPPKPALPVMPLKGGL"
     gene            complement(3101..4072)
                     /locus_tag="PKN55_00575"
     CDS             complement(3101..4072)
                     /locus_tag="PKN55_00575"
                     /inference="COORDINATES: protein motif:HMM:NF017765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent bicarbonate transport family
                     permease"
                     /protein_id="HNX69767.1"
                     /translation="MMHTLLDPAILFFVFGVLAGMLRSNLQIPAPISRFLSLYLLMAL
                     GLKGGFALAHSGLTLEVAGTLGIAVLLAIVVPLLGYPLLRRLLNGYDATALAATYGSV
                     SAVTFITTIQFLENQQIAYGGHMAAAMALMESPAIIMAVLMANRLRQQAGQTGGGHTP
                     TPLGKILHESFTDGAQLLLLGAMLIGLLTGEAGKAAMAPFSVDLFKGMLAFFLLDMGL
                     MTARNLGGLKGKSPWLLAYAILAPLAHAALALGLASLAGASAGNAALLMALAASASYI
                     AVPAVLREAIPEANPSLYFGMSLGLTFPFNILLGIPLYVGVAQAVLG"
     gene            4174..5106
                     /locus_tag="PKN55_00580"
     CDS             4174..5106
                     /locus_tag="PKN55_00580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018749586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HNX69768.1"
                     /translation="MNVTFRQLKVFLAVAEQLNITAAARVCCVTQPTVSMQLRELTAE
                     VGQPLYEQIGKRIYLTAAGEALARTARAMVQEWSAFEQHMDGLKGLTRGRLRLAVVST
                     AKYFVPSLLGDFCQRHPDIEIALEVLNRDGVLARLEANLDDLYIMSMPPAGRALETQR
                     FLPNPLVVIAAATHPLAQRRGLQLADLAGERFILREQGSGTRLACDAHFAQAGFVPQL
                     RLELGSNEAIKRSVAAGMGLAVMSEHALAAQPAEDGVAVLDVAGFPLMSNWWTLYPRG
                     KRLSPVASAFLAHLGEAAGAWYALRHPPGAAAAG"
     gene            5222..6238
                     /gene="dusB"
                     /locus_tag="PKN55_00585"
     CDS             5222..6238
                     /gene="dusB"
                     /locus_tag="PKN55_00585"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011134099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HNX69769.1"
                     /translation="MQIGPYTLKNRLVVAPMAGVTDRPFRVLCKRMGAGMAVGEMLAS
                     NTALWSTKKSLRRADHRGEPDPIIVQIAGGDPAQMAEAARLSVQQGAQIIDINMGCPA
                     KKVCNVAAGSALLRDEALVARILQAVVAAVDVPVTLKTRTGWSRAHNTALRVAKLAED
                     CGIAALALHGRSREDMYHGQAEYDTIRAVKAAVGIPVLANGDIDSPQKARYVLDYTGA
                     DGVMIGRAAQGRPWIFREIQHYLDTGECLPPPEVAEIREVLLAHLDDLYQFYGEYSGC
                     RIARKHIAWYTRGLEGGNAFRQAMYQLEDTASQRAAVAAFFDRLAEGGDRLRYLTESA
                     VCQD"
     gene            6241..6516
                     /gene="fis"
                     /locus_tag="PKN55_00590"
     CDS             6241..6516
                     /gene="fis"
                     /locus_tag="PKN55_00590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017507566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HNX69770.1"
                     /translation="MARTPNTRRNDHNMNSQDDIAASVRQAMHQYFLDLDGEAPQPIY
                     EMVLAQVEKPLIEVVLAHAQGNQTRAAELLGLNRNTLRKKMKQYDLI"
     gene            6593..8170
                     /gene="purH"
                     /locus_tag="PKN55_00595"
     CDS             6593..8170
                     /gene="purH"
                     /locus_tag="PKN55_00595"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HNX69771.1"
                     /translation="MTPITRALISVSDKTGIVEFAQTLVEAGVEILSTGGTAKLLAEK
                     GLPVIEVADYTGFPEMLDGRVKTLHPKVHGGILGRRDLPEHVAKMAEHGIGNIDLVCV
                     NLYPFEATIANPDCTLEDAIENIDIGGPTMVRSAAKNWAHVAIVTDSADYAALSEELR
                     ANAGKLSKATRFALAKKAFTHTAAYDGAISNYLTSLAEGEVTGAPQRVAFPNRLNLQL
                     VKVQDMRYGENPHQAAAFYRDLDPAAGSIANYRQLQGKELSYNNIADADAAWEAVKTF
                     DAPACVIVKHANPCGVAVAADPLSAYKLAFATDTTSAFGGIIAFNREVDAATVEVVSA
                     QFLEVLLAPSFTDEAKALIAAKKNVRVLEIPLAPGANPFELKRVGGGVLVQTPDVRNV
                     TLPELRVVTERAPSEQEMSDLLFAWRVAKFVKSNAIVFCKDGQTAGIGAGQMSRVDST
                     RIAARKAADAGLPLVGAVAASDAFFPFRDGIDVIAEQGIKAIIHPGGSMRDEEVFAAA
                     NEHGIAMVLTGVRHFRH"
     gene            8196..9476
                     /gene="purD"
                     /locus_tag="PKN55_00600"
     CDS             8196..9476
                     /gene="purD"
                     /locus_tag="PKN55_00600"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012698651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HNX69772.1"
                     /translation="MNILVIGSGGREHALAWRIAQSPRAGQVFVAPGNAGTAQDPRLS
                     NVALNSIPELVVFAREQNIAFTVVGPEAPLAAGVVDAFRAAGLRIFGPTQYAAQLESS
                     KDFAKAFMQRHGIPTAGYQTFTDAAAARAYVDGKGAPIVIKADGLAAGKGVVVAMTLA
                     EAHAAIDDMLLGNKMGAAGARVVIEDFLQGEEASFIVMVDGEHVLAMATSQDHKRLLD
                     DDQGPNTGGMGAYSPAPVVTDAVHQKVMERIILPTVRGMQADGHTYTGFLYAGLMIDA
                     HGDPYTIEFNCRFGDPETQPIMARLASDFTVLLEAGIDGKLDTVSADWDPRVAMGVVL
                     AAAGYPDAPRKGDVITGIPAATADSVTFHAGTAFNADGQLVTSGGRVLCAVGLGDTVQ
                     SARETAYAVADAIQFDGRQLRRDIGARALNRQPG"
     gene            complement(9600..10670)
                     /locus_tag="PKN55_00605"
     CDS             complement(9600..10670)
                     /locus_tag="PKN55_00605"
                     /inference="COORDINATES: protein motif:HMM:NF016629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HNX69773.1"
                     /translation="MATPTTAKWGKHARKVSLTPLRRFELKSPCMATDHDSSYKFLFS
                     TPELVRDLIIGFVPDEWLHSLDYSTLEKVPGSYVTEDFRHRADDIVWRVKVGGEWVYL
                     YLLIEFQSSVDKYMALRMMVYQGLLYQDLIKRGEVLADGRLPPILPIVLYNGSQRWTS
                     PTDVFDLIPPTPGLVAQFKPKAKYLLIDENAYSDSELASVKNLVAAVFRIEHPATPEK
                     ISDLLSLLNDWLADRPDLRRMFALWIRATLMRKVEYRIVLPEIDDLQELNIMLAERLE
                     EWALGYEAQGVQKGILQGLQQGMQQGEALLLQRQLTRRFGTLPAAQLTRIAAATPAQL
                     ETWGDRVLDASSLDEVFGDTRH"
     gene            complement(10695..11591)
                     /locus_tag="PKN55_00610"
     CDS             complement(10695..11591)
                     /locus_tag="PKN55_00610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014088494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNX69774.1"
                     /translation="MTTSAPSHRLAYVLLVLTALFWSGNFVLARAMHAAVPPMTLSFM
                     RWLIAFALLAPFSLRHAWRERALLWQCRGRILALSLLGITAFNSLVYTGVQTTTATNA
                     VLLNSFIPILTVLFGALWFGQRLGGRQMLGLAVSFVGVLAIVSHGEWARLVGLNLNQG
                     DLIVFLAMVSWALYTLALRGLPTGLNRFGLLITMVMIGLLAILPFFVWEVTSGRVVEL
                     NTATLATFAYVGVFPSVVAYLFFNYGVARLGPARAAMFIHLMPAFGALLSTVFLGETL
                     AWYHLAGISAIFLGLSLSTRGQ"
     gene            11862..12500
                     /locus_tag="PKN55_00615"
     CDS             11862..12500
                     /locus_tag="PKN55_00615"
                     /inference="COORDINATES: protein motif:HMM:NF028235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNX69775.1"
                     /translation="MHTPALMPPRRRGRPPKSRDALTDTRELLLRAGLETLTEKGFAT
                     AGLDEILGRVGVPKGSFYHYFASKEAFGLALIERYAHFFARKLDRHLLCAELTPLARV
                     QAFVDDAQAGMARYDYRRGCLIGNLGQEMGALPESFRAQLQAVFADWQARLAACLRLA
                     QQAGEVSATADAEQLAAFFWIGWEGAVLRAKLAQSAEPLTVFARFFLAGLPR"
     gene            12550..13536
                     /locus_tag="PKN55_00620"
     CDS             12550..13536
                     /locus_tag="PKN55_00620"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018749348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase activity,
                     zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family oxidoreductase"
                     /protein_id="HNX69776.1"
                     /translation="MFKAVLIEKDDAGYRASVQTLDDAALPEGNVSVRVSHSTLNYKD
                     ALAITGQGPVVRKFPMVPGIDLVGVVETSQHPDYQPGDAVLLNGWGVGESHWGGLAQQ
                     ARLNGDWLIPLPAGLTAQQAMAIGTAGYTAMLCVMTLERYGVQPKDGEVLVTGAAGGV
                     GSVAVALLSQLGYRVTAMTGRLQDADYLRALGAAEVIERGQYAEPGKPLGKERWAGAV
                     DVAGGHVLANVCASMQYQGVVTACGLAAGMALPATVAPFILRGVTLAGVDSVMRPRAD
                     RLVAWQRLTQELSLDKLALIGSQEVGLDEAIGLAARLLKGEVRGRVVVDVNR"
     gene            13648..14226
                     /locus_tag="PKN55_00625"
     CDS             13648..14226
                     /locus_tag="PKN55_00625"
                     /inference="COORDINATES: protein motif:HMM:TIGR01926.1"
                     /note="This protein belongs to a clade of uncharacterized
                     proteins related to peroxidases such as the
                     alkylhydroperoxidase AhpD; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxidase-related enzyme"
                     /protein_id="HNX69777.1"
                     /translation="MSADLTPAISRFPVPELDALPEDLRARILAVQEKSGFIPNVFLT
                     LAHRPDEFRAFFAYHDALMDKPGKLSKAEREMIVVATSNLNQCQYCVVAHGAILRIRA
                     KDPLIADQVAINYRKADISERQKAMLDFAVKVSASAHLVGEADFAALKAHDFDEEDIW
                     DIAAISAFFGMSNRLANVTSMRPNAEFYALGR"
     gene            complement(14426..16216)
                     /locus_tag="PKN55_00630"
     CDS             complement(14426..16216)
                     /locus_tag="PKN55_00630"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014088641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HNX69778.1"
                     /translation="MQLDIDRLIADFGGPSALADALTQAFPQEPVSRAAIYKWRERGS
                     LPLAQLNKLAQLAALRGQTFSFNDYLTGLKPPATTENRIMTDRLIIFDTTLRDGEQSP
                     GAAMTKEEKIRIARQLEKLGVDVIEAGFAAASPGDADAIRAIAEVVKHSTVCSLARAN
                     ERDVRTAGEAIRPAAKGRIHTFIATSPIHMEKKLRMTPDQVVDAAVAAVRIAKEYTDD
                     VEFSAEDAVRSDMDFLVRIFEAVIDAGAKTINVPDTVGYSIPSAWRERFETLISRVRN
                     SDQVIWSTHCHNDLGMAVANSLAAVQGGARQVECTINGLGERAGNASLEEVVMAVKTR
                     RDLFNVDTGIEPTQIVPTSKLVSTITGYPVQPNKAIVGANAFSHESGIHQDGVLKHRE
                     TYEIMSAESVGWSANRLTLGKLSGRNAFKTKLAELGIELSGEEAINAAFARFKDLADR
                     KREIFDEDLHALLSDGDAGHSSDSYKFISLKVATETGETPLANIVFAEHGVEKRAESG
                     GSGPVDAAFKAIESLVNSGAELELYSVNAITKGTESQGEVTVRLARDGRIANGQGADT
                     DIIVASAKAYLSALNRLSQRGERMNPQTEV"
     gene            complement(16443..17204)
                     /gene="pssA"
                     /locus_tag="PKN55_00635"
     CDS             complement(16443..17204)
                     /gene="pssA"
                     /locus_tag="PKN55_00635"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HNX69779.1"
                     /translation="MPQADSRNKLTLRQQSIYLLPNLFTLAALFAGFFAVVQAMNQRF
                     ETAAVAVFIAMVLDGMDGRVARMTHSQSAFGAEFDSLSDMVSFGVAPALIAYEWMLRG
                     LGKLGWMVAFIYCAGAALRLARFNTMIGVTDKRWFIGLPSPAAAALVAGLVWICHAYR
                     YTDLPYLNWITLGFTAFAGCSMVVNTRFWSFKELHIRRKVPFVALLLVVLAILLIVSE
                     PPLVLFGFFVLYSLSGYVMAALRYLRPGPSPTQMP"
     gene            complement(17413..18177)
                     /locus_tag="PKN55_00640"
     CDS             complement(17413..18177)
                     /locus_tag="PKN55_00640"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HNX69780.1"
                     /translation="MTASTRGEAPAEGAEELTRFTTISATDIIHHLRALASNGEPVTI
                     FSNHGRSFIISRVLGVDQKSFCLYFSQSGNSEANRQLLKSERTVFTCSPGGVKTQFLL
                     EDVSTVDYDGQMAFMAPLPNEVTKLQRREFFRIQLPSTAPVSCTLYNYQGGAAITLQV
                     YDISLGGLSLVLNARIPGFDIGTIYNNCELDLREYGVIPLVLKVRNQLVARSTSGAEQ
                     RRVGVEFVELDSRAQTLVQRYIAQQERERRAMGRDD"
     gene            complement(18372..18983)
                     /locus_tag="PKN55_00645"
     CDS             complement(18372..18983)
                     /locus_tag="PKN55_00645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008952113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HNX69781.1"
                     /translation="MPLPFARLLTHVCTALLLACALPACSPTPPSPPAVAFQGRDISG
                     SLTLQDFTLTDHNGQPRKLSEFRGKPVAVYFGYTHCPDVCPTTLAELAQSMRELGPQA
                     DQVQVLFITIDPARDTQALLKSYAPAFDPRFIGLTGSPAQIASVAGQFRALYRRQDGA
                     NGDYTMDHSAGAYLLDRDGKLRVEVPYGSGAATFTHDLRALLQ"
     gene            complement(19088..19732)
                     /locus_tag="PKN55_00650"
     CDS             complement(19088..19732)
                     /locus_tag="PKN55_00650"
                     /inference="COORDINATES: protein motif:HMM:NF025033.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNX69782.1"
                     /translation="MHVSALKHGQLFFDTYLGREADVTIVDIGAQDINGSLRHGAPAG
                     CRYIGVDFVPGPGVDVVLDDPYRLPFADASVDVVVSSSCFEHIEFFWLMFNEALRVLA
                     PGGLLYLNAPANGDFHRYPVDCWRFYPDAGAALARWGQRSGFACVLLESFTAYQDQDI
                     WNDQVAVFLKDRAELARHPQRMLERVGRYMNGRLHGSEAVLAWQAASEDRMKHR"
     gene            19831..20748
                     /locus_tag="PKN55_00655"
     CDS             19831..20748
                     /locus_tag="PKN55_00655"
                     /inference="COORDINATES: protein motif:HMM:NF037279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WcbI family polysaccharide biosynthesis putative
                     acetyltransferase"
                     /protein_id="HNX69783.1"
                     /translation="MLFYGNPAPDAELVVMYGNCQIPFLASLLAAAHGDKGFLCVLNH
                     APPGEEPDRPTPEQMQRCCLYLEQYDSQFDLAAWGVPPEVERYGLPLCRYLREHCPPA
                     CPTITYPSFVMTCMWPFAVTSDPRNVPEPAYVWGRYPYGNRVALEVAASGLRGAQALA
                     AYQQQSAAQMPDLSALLARAERKLWHRDAQCDVKIGDYVWSNFRERHLFLAYAHVRAE
                     AIGELAQRLWRAVQPLLGGDADAAWRRLNAAIDAMPDMDTMEEPIDPLVAQELGLKFY
                     QPDMRFRWFDQRWTFAEYITRYIDYDTSW"
     gene            complement(20781..21833)
                     /locus_tag="PKN55_00660"
     CDS             complement(20781..21833)
                     /locus_tag="PKN55_00660"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNX69784.1"
                     /translation="MTLYEADLQLAVARHLRWMPDRITLDADSLLMEGWALGVWDAQT
                     QCRFLVNGVDFDVLEWPLPSPDLLTVFPDVPQAAHSRFRCRHYLTDGGLSFPGGWARF
                     NVTGPAGEHAWSYRTAWFLADPAQESPLPPAEQIAQVIGTPDPQAFCLGGATMVARFA
                     HLLSERFNRPLSSFTAILDWGCGVGRLTRYLVGQGPAVTGMGSQPAHMQYCRTALPAA
                     HFVAMGDTLASGQFDLVLGLSMLPHLSEAEQDSWLAELQRLTRPGALLLLSVQGLTHM
                     ALYRTPMVHKLEAHRTGWHDMAGHAGLDSPTPSGPDVLHAPDYILAHWGRYFDVLDII
                     EAMAGHQDVVVLRRRA"
     gene            complement(22023..23555)
                     /locus_tag="PKN55_00665"
     CDS             complement(22023..23555)
                     /locus_tag="PKN55_00665"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HNX69785.1"
                     /translation="MAIFSSDLAHSTRRRWTLYAGTLVFLGLFGSWLALDFLHERART
                     LDEMGRLALHKSQLLSSVFGDAFLSADYVLRDIAGRIDPTQDLNPSADHAKARQRQLH
                     KLLHEKLTTVSGLEDLVLFDHNCVFTAVASRPQLLGFRSQQRFCDSRQVNFSEPLHIQ
                     YMPAERSASGQPVVLMSRTLSDDQGRLLGGVMAVMDLGHAQRWIESFSLEPNDLMAIV
                     DNDGIVLARNPPLPAAMGKPTSPPPGYPPFVQMERTTMFWATSPLDHRERIFGLSRLE
                     RFPFIAMVGFDKSRILTGWWHRGWQFLIGFAVLGILAVLVLRAHLTVLRHSEAMRRLA
                     ITDELTGVANRRQLMETGAAEVARARRYQRPLSVLMVDIDHFKHINDRWGHATGDRVI
                     QVMARLLCDTIRQQDHGGRLGGEEFAAILPETDLAGAQTLAERVRVLAASCVRALNDG
                     DAPVSFTVSIGVAMLDANDEGLESLLQRADRALYQAKADGRNRVAVMAAEASSTAHSG
                     TP"
     gene            complement(23708..>24196)
                     /locus_tag="PKN55_00670"
     CDS             complement(23708..>24196)
                     /locus_tag="PKN55_00670"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239815.1"
                     /note="catalyzes the formation of
                     (R)-2,3-dihydroxy-3-methylbutanoate from
                     (S)-2-hydroxy-2-methyl-3-oxobutanoate in valine and
                     isoleucine biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HNX69786.1"
                     /translation="GVIETNFREETETDLFGEQAVLCGGAVELVKAGFETLVEAGYAP
                     EMAYFECLHELKLIVDLMYEGGIANMNYSISNNAEYGEYVTGPEVVTAATKEAMKKAL
                     YRIQSGEYAKMFILEGKTNYPSMTARRRLTADHQIEKVGAQLRAMMPWIAKNKLVDQA
                     KN"
BASE COUNT         4504 a         7563 c         7835 g         4294 t
ORIGIN      
        1 gatgtggaca agccgcgcaa tctggccaag agcgtgacgg tggagtgagt ttgccgcact
       61 tttacccgcc tgatcgggcg gggagtgcta caatcccccc cgcgtcgagt cagccggaca
      121 gtcgctgcct gcttgcaggg agaggaaagt ccgggctcca cagggcagga tgccggttaa
      181 cgaccgggcg ttgcaaggcg acggaaagtg gaacagagag cagaaccgcc gatggccggg
      241 caaccggcac aggcaagggt gaaaaggtgg cccctcgggg tgaatggccg gcaacggctg
      301 ttccggtaag agcgcaccgc ggacgtggcg acacggacgg caggctaaac cccatccgga
      361 gcaagaccaa ataggggaac gttcatgcgg cccgcatggt tcccgggtag gttgcttgag
      421 ccggcgagta atcgtcggcc tagaggaatg actgtcaccg gcggcaacgc cggcacagaa
      481 cccggcttac aggctggctt ggcgcaatgc acacaaaaac gggacacgcc cttggttggg
      541 cgtgtcccgt ttttattgcc aggcgctgat tacttgctct ggcgtttttt acttttgctg
      601 ctggtttttt tcttgacgct catgcccgga tcaatggtcg gctcgggcaa ggcgccggcc
      661 ggtgacagat tcaccaggcc aggctggtta tagccggcca gattggcgct gggcgtgcag
      721 cgcaccgtgg cgcgggcata gcggcgggcg tccagcatgg cgttttccag gccaaacacc
      781 agtttttcaa tgccggggtt cgggcagccc tggcgcacgc cgtaggcgcg ggcctcggtg
      841 cgcagggttt gctccagcac gccttcgcta ttggcgtggg cccagacggt caactcgaca
      901 tcgcggccgc tgagcgcggt gatcttgcgc tccagcacat agccatcgct ggcggtcatg
      961 acgccgccgt ggtagcccac ggcggaacag ccgctcacca cggcggccag gcaggctgct
     1021 accagatatt tcatgaggtg tcctcgcaac acgggtcagc ccggcaggcc gagcaggctg
     1081 cgcacttcgc ggttcagcgg cagcgcgtaa tcataggcgg ccagcaccag attgttaccc
     1141 ggctgcacca ctttcaggtt gacgaacacc atgtcgccgc tcaagccata actgcccagc
     1201 aggatggcac gggcattgtg ctgacgggcc acctcggcga tttcgcgggt cagcgccagc
     1261 tcgccttcgg tgcgacgcat atacatggtg tggcgcagct tcagctcgac catggcgcgg
     1321 ccttgctggg caaagcgcgc cgccacctgt tccgacacca gccggcccag ggtggacgag
     1381 cgctccagcg cgtcgatatt caccagcgtg gccaccagca tcgggccttc attgtccaga
     1441 ttgcgcagtt ggcccagcag cacatccgcc gcgcggtaat tggtgctcac cagctcattg
     1501 cgtgcagcgg cctcataggt gggctcgctg cggctgccgt cggtggcgca ggcgctcagg
     1561 cccagacaca gggcgctggc ggtcagggca agcaggcgct tcatcacagg cctcccttga
     1621 gtggcatcac cggcagcgcc ggcttgggcg gtggcggcgg cagttgcatg tacagcgcca
     1681 aatcactgtc ggtcaggtaa taaatattgc tgacccggcc aacgtactgg ccattggtca
     1741 atgccgatac ggtgatgatc atttccagct gtggcacgcc accacgggcg aattccgaac
     1801 tgaaccagcg atgggcgtca aacgccgcgc ccagggccgc gccagtggcg atgccctgcg
     1861 gcgaggtttg cgtccacagc ccttgcagcg cccagacgcc agcacccaat gtcgtgccgc
     1921 tgacaaagta acggccgtcc aggcgatctg gcgagaattt caccacctgg atgtctactt
     1981 ccagctcctg acgtggcgcc ggggctggcg ggatgaccgg catggcgtct acgggggcca
     2041 tttgggcgac tggtttgggg gcagtggcca gataggcttt tttgccatat ttttggcgct
     2101 tgaccacgcg cggcgtcggg gcgctgacca gtggcgccgg catcggcggc ggggtgtagg
     2161 ccatcgacgg ctgcggtgcg ctgccgtatg gaatcatttg cacgccctga ttcaccagcg
     2221 aagtggtcag cagggtgtgg aaggcgcgcg agaacggggt ggcttcacgc ggcgggcgca
     2281 gcaccagctg gttgcaatcc ggataggcgg cgatgcagcc cttgcccagg cgcagcgagg
     2341 tcaccaaggt ggtggcggcg tctttggcga tggcgtccca gtgcgcgccg gcttgcagtt
     2401 tatgttgctt gctggtgggg aaattggtcg ctaccggggc ttcgctgtaa ggcgtggtgc
     2461 aggcgctcag cagcaggctg gcgcacaggg caagaccggg cagggccggg cgcagaaggg
     2521 tgggtttcat tgtgggcggc tcacatcggc aattcgggat tggcggaacg ccggacatac
     2581 tatcatggca tgcgtagcgt gtgcgcaggg cgcgcggcgg catgccgcat cgctctggcc
     2641 gtgttgctga tatcggccgt atgcggcggg tcttgtgcgt cttgctggat gtgcgtgtgg
     2701 agccacgctg cgcgcgggcc tgattttggg cgggagtgcg tcccgcatgc cgccctactt
     2761 tctttgcttc gccaaagaaa gtaggcaaag aaaggcgacc ccaggacgcg cgcccgtccg
     2821 ccgcctgcgg cgtcggtggt cccctgtgct tctcgccagc ccaggccgtc ccggaaactc
     2881 gcgtcagctg cgctgccgct caaacaaccg ggccggactt cccctgggct ggctgcgatg
     2941 ctcggcgcgc tttatgggga ggcaaagcgg ctaagcacga cctccaggtt catgagatga
     3001 atggccgccc aacacaaccc tgctggtgtc gttgcgcggc cttgccgttg tctgcgcaca
     3061 cctggccagg atcagcttcg ctgggttttg tgagcggcgc ctagcccaac accgcctgcg
     3121 ccacccccac atacagcgga atccccagca ggatgttgaa cggaaacgtc agccccagcg
     3181 acatgccaaa gtacagcgag ggattggctt ccgggatggc ttcgcgcagc actgccggca
     3241 cggcgatata cgaggcgctg gcggccagcg ccatcagcag cgcggcattg cccgcgctgg
     3301 cgccggccag gctggccagg cccagcgcca gcgccgcgtg cgccagcggg gcgaggatgg
     3361 cgtaggccag cagccagggc gatttgccct tcagcccgcc cagattgcgc gcggtcatca
     3421 ggcccatgtc cagcaggaag aaagccagca tccccttgaa caaatccacc gagaacggcg
     3481 ccatcgccgc cttgccggcc tcgccggtca acagcccgat cagcatggcg cccagcagca
     3541 gcaactgcgc gccgtcggtg aaggattcgt gcaggatttt gcccagcggc gtcggcgtgt
     3601 ggccgccgcc ggtctggccg gcttgctggc gcagccggtt ggccatgagc accgccatga
     3661 tgatggcggg cgattccatc agcgccatcg ccgccgccat atggccgcca taggcgatct
     3721 gttggttttc cagaaactga atggtggtga tgaaggtcac tgcgctgacc gagccatacg
     3781 tcgccgccag cgcggtggcg tcgtagccat tcagcagccg gcgcaagagc ggatagccca
     3841 gcagcggcac aacaatcgcc agcaacacgg caatgcccag cgtgccggcc acttccagcg
     3901 tcagcccgga gtgagccagg gcaaagccgc ctttcaggcc cagcgccatc agcaggtata
     3961 aagacaaaaa ccgcgaaatc ggcgcgggga tttgcaaatt ggagcgcagc atgccggcca
     4021 gcacgccaaa cacaaaaaac agaatggccg ggtccagtag ggtgtgcatc atcacctctt
     4081 atcagcaatc ggggcaggaa ttccccgtat gctagggcgg ctgatggata ataaaaaatc
     4141 aataattcag attgtatcca taggtaaata ctgatgaatg tcacctttcg ccagctcaag
     4201 gtgtttctgg ccgtggccga gcagctcaat atcaccgccg ccgcgcgcgt ctgctgcgtg
     4261 acccagccca ccgtgtcgat gcagctgcgc gagctgaccg ccgaagtcgg ccagccgctg
     4321 tacgagcaga ttggcaagcg gatttacctg accgccgccg gcgaggcgct ggcgcgcacg
     4381 gcgcgcgcca tggtgcagga atggtcggcg tttgagcagc atatggacgg cctgaaaggg
     4441 ctgacgcgcg gccggctgcg gctggcggtg gtgagcaccg ccaagtattt tgtccccagc
     4501 ctgctgggcg atttttgcca gcgtcacccg gatatcgaaa tcgccctgga agtgctcaac
     4561 cgcgacggcg tgctggcccg gctggaggcc aatctggatg atttgtacat catgtccatg
     4621 ccgccggccg gccgcgcgct ggaaacccag cgctttttgc ccaatccgct ggtggtgatt
     4681 gccgccgcca cccacccgct ggcgcagcgg cgcggcctgc aactggcgga cttggccggc
     4741 gagcgtttta tcctgcgcga acaaggctcc ggcacccggc tggcctgcga cgcccacttt
     4801 gcccaagcgg gttttgtgcc gcagctgcgg ctggagctgg gcagcaacga ggcgatcaaa
     4861 cgctcggtgg cggccggcat ggggctggcg gtgatgtccg agcacgcgct ggccgcccag
     4921 ccggcggaag acggcgtggc ggtgctggac gtggccggct ttccgctgat gtccaactgg
     4981 tggaccttat acccgcgcgg caagcggctc tcgccagtgg ccagcgcctt tctggcccac
     5041 ctgggcgagg ccgccggcgc ctggtatgcg ctgcgtcatc cgcccggcgc ggcggcggct
     5101 ggctgaatga tgcgccgcct gtggtgggat tggtcaaaaa ataagcaaat tttttttacc
     5161 gccaggtgaa ttcgcattaa aatgccctcc cttttctcgc gcacctcatt gaattttcag
     5221 catgcagatt ggcccgtaca ccctcaagaa ccgcttggtc gtggccccga tggccggggt
     5281 gaccgaccgg ccgtttcgcg tgctgtgcaa acgcatgggc gccggcatgg cggtgggcga
     5341 gatgctggcc tccaacaccg cgctgtggtc caccaaaaaa agcctgcgcc gcgccgacca
     5401 tcgcggcgag cctgatccga tcatcgtgca gatcgccggc ggcgacccgg cgcaaatggc
     5461 cgaggccgcg cggctgtcgg tgcagcaggg cgcgcagatc atcgacatca atatgggctg
     5521 cccggccaaa aaggtgtgca atgtcgccgc cggctcggcg ctattgcgcg acgaagcgct
     5581 ggtggcgcgc atcctgcaag cggtggtggc ggcggtggac gtgccggtga cgctgaaaac
     5641 ccgcaccggc tggagccggg cgcacaacac cgcgctgcgc gtggccaagc tggccgaaga
     5701 ctgcggcatc gccgcgctgg cgctgcacgg ccgcagccgc gaagacatgt accacggcca
     5761 ggccgaatac gacaccatcc gcgcggtcaa ggcggcggtg ggcattccgg tgctggccaa
     5821 tggcgacatc gacagcccgc aaaaagcccg ctatgtgctt gattacaccg gcgccgacgg
     5881 cgtgatgatc ggccgcgccg cccagggccg gccgtggatt tttcgcgaaa tccagcacta
     5941 tctggacacg ggcgaatgcc tgccgccgcc agaagtcgcc gaaatccgcg aggtgctgct
     6001 ggcccacctg gacgacctct accagtttta cggggaatac tccggctgcc gcatcgcccg
     6061 caagcatatt gcctggtaca cgcgcgggct ggagggcggc aacgcctttc gccaggccat
     6121 gtaccaactg gaagacaccg ccagccagcg cgccgccgtg gccgcgttct ttgaccggct
     6181 ggccgaaggc ggcgaccgcc tgcgctacct cacggaaagc gcggtttgcc aggactgaat
     6241 ctggccagaa caccaaacac tcgcagaaac gaccataaca tgaacagcca agacgacatt
     6301 gccgcctcgg tgcgccaggc aatgcatcaa tacttccttg atctggacgg cgaagccccg
     6361 cagcccatct acgaaatggt gctggcgcag gtggaaaaac cgctgatcga ggtggtgctg
     6421 gcgcacgcgc agggcaacca gacccgcgcc gccgaactgc tcgggctgaa ccgcaacacc
     6481 ctgcgcaaga aaatgaagca atacgacctc atctgaccgc cccccggcgg ccgatgcgct
     6541 cccccgttga ctgttgaaca ctccgatact tttgccaagg cgaatcgtga ccatgactcc
     6601 catcacccgc gcgctgatca gcgtttccga caaaaccggc atcgtcgaat ttgcccagac
     6661 cctggtcgag gccggcgttg aaatcctctc cactggcggc accgccaaac tgctggcgga
     6721 aaaaggcctg ccggtgatcg aagtggccga ctacaccggc tttccggaaa tgctcgacgg
     6781 ccgggtgaaa accctgcacc ccaaggtgca cggcggcatt ctgggtcgcc gcgacctgcc
     6841 cgagcatgtg gccaagatgg ccgagcacgg catcggcaat attgatctgg tgtgcgtcaa
     6901 cctgtacccg tttgaagcca ccatcgccaa cccggactgc acgctggaag acgccatcga
     6961 aaacatcgac atcggcggcc cgaccatggt gcgctcggcg gccaaaaact gggcgcatgt
     7021 ggccatcgtc accgacagcg ccgactacgc cgcgctgagc gaagagctgc gcgccaacgc
     7081 cggcaagctg tccaaggcca cccgctttgc cctggccaag aaagccttca cccacaccgc
     7141 cgcctacgat ggcgcgattt ccaactacct gaccagcctg gccgaaggcg aagtcaccgg
     7201 cgcgccgcag cgcgtggcct tccccaaccg cctgaatctg caactggtca aggtgcagga
     7261 catgcgctac ggcgaaaacc cgcaccaggc cgctgctttc taccgcgacc tcgacccggc
     7321 cgccggcagc atcgccaact accgccagct gcaaggcaag gaactgtcgt acaacaatat
     7381 cgccgacgcc gacgccgcct gggaagccgt caagactttc gacgccccgg cctgcgtcat
     7441 cgtcaagcac gccaacccgt gtggcgtggc ggtggccgcc gatccgctca gcgcctacaa
     7501 actggccttc gccaccgaca ccaccagcgc ctttggcggc atcatcgcct tcaaccgtga
     7561 agtggacgcc gccaccgtgg aagtggtcag cgcccagttc ctggaagtgc tgctcgcccc
     7621 gtcgttcacc gacgaagcca aggcgctgat cgccgccaag aaaaacgtgc gcgtgctgga
     7681 aatcccgctg gcgccgggcg ccaacccgtt cgagctcaag cgcgtcggcg gcggcgtgct
     7741 ggtgcaaacc ccggacgtgc gcaacgtcac cctgccggaa ctgcgcgtgg tcaccgagcg
     7801 cgcgcccagc gagcaagaaa tgtccgacct gctgtttgcc tggcgcgtgg ccaagttcgt
     7861 caagtccaac gccattgtgt tctgcaagga tggccagacc gccggcattg gcgccggcca
     7921 gatgagccgg gtggattcca cccgcatcgc cgcgcgcaag gcggctgacg ccggcctgcc
     7981 gctggtgggc gccgtggccg cttcggatgc cttcttcccg ttccgcgacg gcatcgacgt
     8041 gatcgccgaa cagggcatca aggccatcat ccatccgggc ggctcgatgc gcgacgaaga
     8101 agtgttcgcc gccgccaacg agcacggcat cgccatggtg ctgaccggcg tgcgccactt
     8161 ccgccattga ttcgacccga ccgcaaggat agaccatgaa tattctcgtc atcggctccg
     8221 gcggccgcga gcacgcgctg gcctggcgca tcgcccagtc gccgcgcgcc ggccaggtgt
     8281 ttgtcgcccc cggcaacgcc ggcaccgccc aggacccgcg cctgagcaat gtcgcgctca
     8341 acagcatccc cgaactggtg gtgtttgccc gcgaacagaa catcgccttt accgtggtcg
     8401 gcccggaagc gccgctggcc gctggcgtgg tggacgcttt ccgcgccgcc ggcctgcgca
     8461 tctttggccc cacccagtac gccgcccagc tggaaagctc gaaagacttc gccaaggcct
     8521 tcatgcagcg ccacggcatc cccaccgctg gctaccagac tttcactgac gccgccgccg
     8581 cccgcgccta tgtggacggc aagggcgcgc cgattgtcat caaggccgac ggcctggccg
     8641 ccggcaaggg cgtggtggtg gcgatgacgc tggcagaagc gcacgccgcc atcgacgaca
     8701 tgctgctggg caacaagatg ggcgccgccg gcgcgcgcgt ggtgatcgaa gacttcctgc
     8761 aaggcgaaga agccagcttc atcgtcatgg tggacggcga gcatgtgctg gccatggcca
     8821 ccagccagga ccacaagcgc ctgctggacg acgaccaagg cccgaacact ggcggcatgg
     8881 gcgcctatag cccggccccg gtggtcaccg acgccgtgca ccagaaggtg atggagcgca
     8941 tcattctgcc gaccgtgcgc ggcatgcagg ccgatggcca cacctacacc ggcttcttgt
     9001 acgccggcct gatgatcgac gcccacggcg acccgtacac catcgagttc aactgccgct
     9061 ttggcgaccc ggaaacccag ccgatcatgg cccgtctggc cagcgacttc accgtgctgc
     9121 tggaagccgg catcgacggc aagctggaca cggtcagcgc cgactgggat ccgcgcgtgg
     9181 ccatgggcgt ggtgctggcc gccgccggct acccggacgc cccgcgcaag ggcgacgtga
     9241 tcaccggcat cccggccgcc accgccgaca gcgtcacctt ccacgccggc accgccttca
     9301 acgccgatgg ccagctggtc accagcggcg ggcgggtgct gtgcgccgtg ggcctgggcg
     9361 acacggtgca aagcgcgcgg gaaaccgcct acgccgtggc cgacgccatc cagtttgacg
     9421 gccgccagct gcgccgcgat attggcgcac gggcattgaa ccgccagcct ggctgatcgt
     9481 tgccctgcca catcaaaacg ccccgcgccg gtttgccgac gtggggcgtt ttgatgttat
     9541 cccgagcgct gggcgctgcg ccaggatgag tcaagcagca cggtgaagcg gtgtatcgct
     9601 caatgccgag tgtcgccaaa cacctcatcc agcgagcttg catccagcac tcgatcaccc
     9661 caggtttcca gctgggcggg ggtggcagcg gcaatacggg tcagctgcgc ggccggcagc
     9721 gtgccgaaac ggcgagtcag ctgtcgttgc aacaacaaag cctcgccttg ctgcatccct
     9781 tgctgtaatc cctgcaagat gcctttttgc acgccctgcg cctcatagcc cagcgcccat
     9841 tcttccagtc gttccgccag catgatgttc agctcctgca aatcatctat ctcaggtaat
     9901 acaatacgat actccacttt gcgcatcaat gtagcgcgta tccacagtgc aaacattctg
     9961 cgcagatcag gccggtcggc aagccaatcg ttcagcaggc tgagcaggtc gctgattttt
    10021 tctggtgtgg ctgggtgctc aatccggaag acggcggcca ccaggttttt gacggatgcc
    10081 aattcgctgt cgctgtaggc gttttcatcg atcagcaggt atttggcctt aggcttgaat
    10141 tgcgccacca ggcctggagt gggaggaatc aaatcaaaga catcggtggg cgatgtccag
    10201 cgttggctgc cgttgtagag cacgatgggc aagatgggcg gcagacggcc atcggccagc
    10261 acttcgccgc gcttgatcag atcctgatag agcaggcctt gatacaccat catgcgtagc
    10321 gccatgtact tatcaacgct gctctgaaac tcaatcagca gatacagata cacccactca
    10381 cccccgacct tgacccgcca gacgatgtcg tcggcgcggt gacgaaagtc ctcggtgacg
    10441 tagctgccgg ggactttttc cagggtggaa taatccaggc tgtgcagcca ttcgtccggg
    10501 acgaaaccga tgatcaggtc acgcaccagt tcgggcgtgg aaaaaagaaa cttgtagctg
    10561 ctgtcgtgat cggtggccat gcaggggctt tttaactcaa aacggcgaag tggggttaac
    10621 gaaacctttc tggcgtgctt accccacttt gccgtagtcg gtgtggccat tgtgctgagt
    10681 gtgtcgcacc ctgtttactg cccgcgcgtg gacagcgaca gccccagaaa aatcgcgctg
    10741 atgccggcca ggtgatacca agccagggtt tcgcccagaa acactgtgga caatagcgcg
    10801 ccaaacgccg gcatcaggtg gatgaacatc gccgcgcgcg ccgggcccag gcgggccacg
    10861 ccatagttga agaacaaata agccaccacc gacggaaaca cgcccacata ggcaaaggtg
    10921 gccagggtgg cggtgttcag ctcgaccacg cggccgctgg ttacttccca gacaaaaaac
    10981 ggcaggatgg ccagcaggcc gatcatcacc atggtgatca gcaggccaaa gcggttcagc
    11041 ccggtgggca ggccgcgcag cgccagggtg tacagcgccc agctgaccat ggccagaaac
    11101 acgatcaaat cgccctggtt gaggttcagc ccaaccagcc gcgcccattc gccgtgcgac
    11161 acaatcgcca gcacgcccac aaacgatacc gccagcccca gcatttgccg cccgcccaga
    11221 cgctggccaa accacagcgc gccaaacagc acggtgagaa tggggataaa cgagttcagc
    11281 agcacggcgt tggtggcggt ggtggtctgc acgccggtgt acaccagcga attaaacgca
    11341 gtgatgccca agagcgacag cgccagaatc cggccgcgac actgccacag cagcgcgcgt
    11401 tcgcgccagg cgtggcgcag cgaaaatggc gccagcaagg caaaggcaat cagccagcgc
    11461 atgaaagaaa gcgtcatcgg cggcacggcg gcgtgcatgg cgcgggccag gacaaaatta
    11521 cccgaccaaa acagcgcggt gagcaccagc agcacatagg ccaggcgatg ggagggggca
    11581 gaagtcgtca tcagcctaga gtgccaagga ttgcccaact cggcaagcgc tggcttggtg
    11641 gtctggccaa tttgcgccgc gcacaaaact cagcgcagcg gccctggctg ggaacacgac
    11701 cgcagacagg acagggagta cggcaaggtg acataccacc accaggggct ttgtgagccg
    11761 ctcttcgcat gcgggtgagt gcgtgggatg gcctgtgaca tgcattgccc ggtggggcga
    11821 cccgcttgct tttttcaaga cgctcagtct aatattggtg catgcacaca cctgccctga
    11881 tgcccccccg ccgccgaggc cgcccgccca agtcgcgcga cgcgctgacc gacacgcgcg
    11941 agctcttgct gcgcgccggg ctggaaaccc tcaccgaaaa aggcttcgcc accgccgggc
    12001 tggatgaaat cctgggccgc gtcggcgtgc ccaagggctc gttttatcac tactttgcca
    12061 gcaaagaggc gtttggcctg gcgctgatcg agcgctacgc ccactttttt gcccgcaagc
    12121 tggaccgcca cttactgtgc gccgagctga cgccactggc gcgggtgcag gcctttgtcg
    12181 atgacgccca ggccggcatg gcgcggtatg actaccggcg cggctgtttg attggcaatc
    12241 tgggccagga gatgggcgcg ctgccagaaa gctttcgcgc ccagctgcag gcggtgttcg
    12301 ccgactggca ggcgcggctg gccgcctgcc tgcgcctggc gcagcaggcg ggcgaagtgt
    12361 cggccacggc ggacgccgag caactggcgg cgtttttctg gatcggttgg gaaggcgcgg
    12421 tgttgcgcgc caagctggcg caaagcgccg agccgctgac ggtgtttgcc cggttttttc
    12481 tggccggatt gccgcgctga atttttttgc caataagtag acagtcagtc taaatacaag
    12541 ggagaaacca tgttcaaggc agtgctgatt gaaaaagacg acgccggcta tcgcgccagc
    12601 gtgcagacgc tggacgatgc cgccttgccc gagggcaatg tcagcgtgcg cgtcagccat
    12661 tccacgctca actacaaaga cgcgctggcc atcaccggcc aaggcccggt ggtgcgcaaa
    12721 ttccccatgg tgcccggcat tgatctggtc ggcgtggtgg agaccagcca gcacccggat
    12781 tatcagccgg gtgatgcggt gttgctgaat ggctggggcg tgggcgaaag ccattggggc
    12841 gggctggcgc aacaagcgcg cttgaatggc gattggctga ttccgctgcc ggcaggcttg
    12901 accgcccagc aggcgatggc gattggcacc gccggctaca ccgccatgct gtgtgtgatg
    12961 acgctggagc gctatggcgt gcagcccaaa gatggcgagg tgctggtgac cggtgcggcg
    13021 ggcggcgtgg gcagtgtggc ggtggccttg ctcagccagt tgggctatcg ggtgacggcg
    13081 atgaccggcc gcttgcaaga cgccgactat ctgcgcgcgc tgggcgcggc ggaggtgatc
    13141 gagcgcgggc agtacgccga gcctggcaag ccgctgggca aagagcgctg ggcgggcgcg
    13201 gtggacgtgg cgggcggcca tgtgctggcc aatgtctgcg ccagcatgca ataccagggc
    13261 gtggtgacgg cgtgcggcct ggccgccggc atggcgctgc cggccaccgt ggcgccgttt
    13321 attttgcgcg gcgtcaccct ggccggggtg gacagcgtga tgcgcccgcg cgccgaccgg
    13381 ctggtggcct ggcagcggct gacgcaggaa ttgtcgttgg acaagctggc gctgatcggc
    13441 agccaggaag tggggctgga tgaggcgatt ggcctggcgg cgcgcctgct gaagggtgag
    13501 gtgcgcggcc gcgtggtggt ggacgtcaac cgttaagcgc ggctaacacg cgctcaccac
    13561 atgcccctct ggccatgtgg tgtggcgcac actgttgccg cgtcacccgg cggggcagag
    13621 atatttttta tttttcagga atttgtcatg agtgctgacc tcaccccggc catcagccgt
    13681 tttcccgtgc cggaactgga cgccctgccc gaagacctgc gcgcgcgcat cctggccgtg
    13741 caggaaaaat ccggttttat ccccaatgtg ttcctcaccc tggcgcaccg gccggatgag
    13801 tttcgcgcct tttttgccta ccacgacgcg ctgatggaca agcccggcaa gctgagcaag
    13861 gccgagcgcg agatgattgt ggtggccacc agcaatctga accaatgcca gtattgcgtg
    13921 gtggcgcatg gggcgatttt gcgcattcgc gccaaagacc cgctgattgc cgaccaagta
    13981 gcgatcaact accgcaaggc ggacatcagc gaacggcaaa aagccatgct ggactttgcc
    14041 gtcaaggtgt cggccagcgc gcatctggtg ggcgaggcgg attttgccgc gctgaaggcg
    14101 catgattttg acgaagaaga catctgggac atcgccgcaa tttcggcgtt ttttggcatg
    14161 tccaaccggc tggccaatgt gaccagcatg cggccaaatg ccgagtttta cgcactgggc
    14221 cgctaagcgc cgctcacata accctcatgg cgtggttgca tggtctcgcc gtactgctga
    14281 cctctgtctg tgcacgcctc gccaagtgcg ttgcttagcg cggttcaaac gcgctgaggc
    14341 gggcaggtgg atcacacctg cccgccgggc cgtggccggg gatcacccgg cgaagcggtt
    14401 tcgtgagtca ggggcggatc gcgcctcaga cttcggtttg tggattcatg cgctcgccac
    14461 gctggctcag gcggttgagc gcggacaggt aagccttggc gctggcgaca atgatgtcgg
    14521 tgtcggcgcc ctggccgttg gcgatgcggc cgtcgcgcgc caggcgcacg gtgacttcgc
    14581 cttgcgattc ggtgcccttg gtgatggcgt tcaccgagta cagctccagt tcggcgccgc
    14641 tgttcaccag cgattcaata gccttgaacg ccgcgtccac cgggccggag ccgccggatt
    14701 cggcgcgctt ttccacgccg tgttcggcaa acacgatatt ggccagcggg gtttccccgg
    14761 tttcggtcgc cactttcagc gagatgaatt tgtagctgtc actgctgtgg ccggcgtcgc
    14821 cgtcggacag cagggcgtgc aggtcttcgt cgaagatttc gcgcttgcgg tcggccaggt
    14881 ccttgaagcg ggcaaaagcg gcgttgatgg cttcttcgcc agacaattca atgcccagtt
    14941 cggccagctt ggtcttgaac gcattgcggc cagacagctt gcccagggtc aggcggttgg
    15001 cgctccagcc caccgactcg gcgctcatga tttcgtaggt ttcgcggtgc ttgagcacgc
    15061 catcctggtg gatgccggat tcgtgcgaga aggcgttggc gccaacaatc gccttgttcg
    15121 gctgcaccgg gtagccggtg atggtggaca ccagcttgga ggtcggcacg atctgggtgg
    15181 gctcgatgcc ggtatcgaca ttgaacagat cacggcgggt tttgaccgcc atcaccactt
    15241 cttccagcga ggcattgccg gcgcgctcgc ccaggccgtt gatggtgcat tccacctgac
    15301 gggcgccgcc ctgcacggcg gccagcgagt tggccaccgc catgcccagg tcgttgtggc
    15361 agtgggtgga ccagatcacc tggtcgctgt ttcgcacccg gctgatcagc gtttcaaagc
    15421 gctcgcgcca ggccgacgga atgctgtagc ccacggtgtc cggcacattg atggtcttgg
    15481 cgccggcgtc gatcaccgct tcaaagatgc gcaccagaaa atccatgtcc gagcgcacgg
    15541 cgtcttcggc ggagaattcc acgtcgtcgg tgtattcctt ggcgatgcgc acggcggcca
    15601 cggcggcgtc caccacctgg tccggcgtca tgcgcagctt tttctccatg tggatcgggc
    15661 tggtggcgat gaaggtgtgg atgcggccct tggcggccgg gcggatggcc tcgccggcgg
    15721 tgcgcacgtc gcgctcgttg gcgcgcgcca ggctacagac ggtggagtgc ttgaccactt
    15781 cggcaatggc gcggatggcg tcggcgtcgc cggggctggc ggcggcaaag ccggcctcga
    15841 tcacatcgac acccagcttt tccagctggc gggcgatgcg gattttttct tccttggtca
    15901 tcgccgcgcc ggggctttgt tcgccatcgc gcaaggtggt gtcaaaaatg atgaggcggt
    15961 cagtcatgat gcgattctcc gtcgtcgccg gcggcttcag gccggtcaaa taatcgttga
    16021 agctgaaagt ctggccgcgc agggcggcca gttgggccag tttgttcagt tgcgccagcg
    16081 gcaggctgcc gcgttcgcgc cacttgtaaa tcgccgcccg ggaaaccggc tcttgcggaa
    16141 aagcctgggt gagtgcgtca gccagggcgg acgggcctcc gaaatcggca atcaagcggt
    16201 caatgtccaa ttgcatggcg gtttccttgt cttgctgtgt cacaggatag cgaatcattg
    16261 gacacggagt caaataattt ctgtcgattt cggccagaaa atgcgccttc cgacggattt
    16321 agtctaattt tcttggacat tatgtctaag ttttttgatt ttgtctggtg gctggacatg
    16381 gctccccatc tgaaatgcaa aaaaggtggc cgtagccacc ttttttacca tccctatcac
    16441 gctcagggca tttgcgtggg cgacggcccg gggcgcaggt agcgcagggc agccatcaca
    16501 tagccagaca ggctatacag cacgaaaaag ccaaacagca ccagcggcgg ctcggacaca
    16561 atcagcaaaa tggccagcac caccagcagc aaggccacaa acggcacttt gcggcggatg
    16621 tgcagctctt taaagctcca gaagcgggtg ttgaccacca tcgagcagcc ggcaaaggcg
    16681 gtaaagccca gggtgatcca gttcaggtaa ggcaggtcgg tgtagcggta ggcgtggcaa
    16741 atccacacca ggccggccac cagcgccgcc gctgccgggc tgggcaggcc gatgaaccag
    16801 cgcttgtcgg tgacgccgat catggtgtta aagcgcgcca gccgcagtgc ggcgcccgcg
    16861 cagtagatga aagccaccat ccagcccagc ttgcccaggc cgcgcagcat ccattcgtaa
    16921 gcgatcagcg ctggcgccac gccaaagctg accatgtcgg agaggctgtc gaattcggcg
    16981 ccaaaggcgc tttggctgtg ggtcatgcgc gccacgcggc catccatgcc gtccagcacc
    17041 atggcgatga acaccgccac ggcggcggtt tcaaagcgct ggttcatggc ttgcaccacg
    17101 gcaaaaaagc cggcaaacaa ggcggccagg gtaaacagat tgggcagcag gtaaatgctt
    17161 tgctgccgga gggtgagttt attgcgggaa tcggcttgcg gcatgaatgg ctcgtcaggc
    17221 cagcacaggg cggcgcggcg cgctgcccgg cgcacgatca gtgcgccggc tcggccagaa
    17281 tggttgcggt ggcggacact ttatcaccaa tgacggatga cagccatggg gcacatgggt
    17341 gaccagcatt ccaccacggg cggcagactg ctttgccggt atggcggccg gcaaggcgca
    17401 gagacggtgt gttcagtcgt cgcgacccat ggcgcggcgt tcgcgctctt gctgggcgat
    17461 atagcgttgc accagggttt gtgcacgcga atccagttcg acgaactcca cccccacccg
    17521 acgttgttcg gcgccgctgg tgctgcgcgc taccagctga ttacgcactt tcagcaccag
    17581 tggaatcacg ccgtattcgc gcaggtccag ttcgcaatta ttgtagatgg tgccgatgtc
    17641 aaaaccgggg atgcgggcat tgagcaccaa cgacagcccg cccaggctga tgtcgtacac
    17701 ttgcaaggta atggctgcgc cgccctggta gttgtacagc gtgcagctga ctggcgccgt
    17761 actgggcaac tggatgcgga agaattcgcg ccgttgcagt ttggtcactt cattgggcag
    17821 cggcgccatg aaggccatct ggccgtcgta atccaccgtg ctgacgtctt ccagcaggaa
    17881 ctgggttttc actccgccgg gcgagcaggt gaacaccgtg cgctcgctct tgagcaactg
    17941 gcgattggct tccgagttgc cgctctggct gaaatacaga cagaagcttt tttggtccac
    18001 gcccagcacg cgcgagatga tgaagctgcg gccgtggttg gaaaaaatgg tcaccggctc
    18061 gccgttgctg gccagcgcac gcaaatgatg aatgatgtca gtggccgaga tggtggtgaa
    18121 ccgggtgagc tcttcggcgc cttcggcggg cgcttcgcca cgggtggatg cagtcacggt
    18181 gatggccttg gacggtgaac aaaacgagtg ccctgtgacg acaggcccgg atcgcaagcg
    18241 atgccgggcg gacgaaaagg gcgaaaaggc cgccagacga catgggtcat accgtggttt
    18301 tacttacggc ctctgactaa attttacacg cttttaagcc gcccacaaaa cccggtgcgc
    18361 gctttatcag gctattgcag caaggcacgc agatcgtggg tgaaggtcgc tgcaccactg
    18421 ccataaggca cttccacacg caattttcca tcccggtcca gcaggtaagc cccggcgctg
    18481 tggtccatgg tgtagtcgcc gttggcgcca tcctgacggc gatacaaggc gcggaattgc
    18541 ccggctacac tggcaatttg cgcgggcgag ccggtcaggc cgataaagcg ggggtcaaat
    18601 gccggggcat aacttttcag cagcgcctgg gtgtcgcgcg ccgggtcgat ggtgatgaac
    18661 agcacctgca cctggtcggc ttgcgggccg agttcacgca tgctttgcgc cagctcggcc
    18721 agcgtggtcg ggcacacgtc cgggcagtgg gtatagccaa agtacaccgc caccggtttg
    18781 ccgcgaaact cggacagctt gcgtggctgg ccgttgtggt cggtgagggt gaaatcctga
    18841 agggtaagcg agccgctgat atcccgcccc tgaaacgcca cggccggcgg gctgggcggc
    18901 gtgggtgagc aggcgggcag ggcgcaagcc aacaagagtg cagtgcaaac gtgggtgagc
    18961 aggcgggcaa aaggcaacgg catgattcag gatccgtaaa atcagcggtg aaagtgggtg
    19021 gaaagtgcgc agcatagcgt aatgccaagg ggcttggctg cggcgttttg tcgcaagtga
    19081 cctggggtca gcggtgtttc atccggtctt cgctggccgc ctgccaggcc agcaccgcct
    19141 cgctgccatg cagccggcca ttcatatagc gcccgacacg ctccagcatg cgctgagggt
    19201 ggcgggccag ctcggcgcga tccttgagga acaccgccac ctggtcgttc cagatgtcct
    19261 ggtcctgata ggcggtgaat gactccagca gcacgcaggc aaatccactg cgctggcccc
    19321 agcgcgccag cgcggcgccg gcatccgggt aaaaccgcca gcagtccacc gggtagcggt
    19381 gaaaatcgcc attggccggg gcgttcagat acagcagtcc gcccggcgcc agcacgcgca
    19441 gcgcttcgtt gaacatcagc cagaaaaatt cgatgtgctc aaaacacgag ctgctgacca
    19501 ccacatccac gctggcgtcg gcaaacggca ggcgatacgg gtcgtccagc accacatcca
    19561 cgccggggcc gggcacaaaa tccaccccga tatagcggca gccggccggc gcgccatggc
    19621 gcaggctgcc attgatgtct tgcgcgccaa tatcgacgat ggtgacgtcg gcctcgcggc
    19681 caagataggt gtcaaaaaac agctgtccgt gtttcaatgc gctgacatgc atgatgattc
    19741 ctcaaatctg tcttgccact ggcgctgctg agctgccgcc gcgagggggc ggcaagcggc
    19801 gtctggcatt ttctggagca cgctgcaccc atgttgtttt acggcaatcc ggcgcccgac
    19861 gccgaactgg tggtgatgta tggcaactgc caaatcccgt ttttggccag tttgctggcc
    19921 gccgcccatg gcgacaaagg ctttctttgc gtgctgaacc acgcgccgcc cggcgaagag
    19981 cccgaccgcc ccacccccga gcagatgcag cgctgctgcc tgtatctgga gcaatacgac
    20041 agccagttcg atctggccgc ctggggcgtg ccgccggagg tggagcgcta cggcctgccc
    20101 ttgtgccgct acctgcgcga gcattgtccg cccgcgtgcc ccacgatcac ctaccccagc
    20161 tttgtgatga cctgcatgtg gccgttcgcc gtcaccagcg acccgcgcaa tgtgcccgag
    20221 ccggcttatg tctgggggcg ttacccctat ggcaaccggg tggcgctgga agtggcggcc
    20281 agcggcttgc gcggcgcaca ggcgctggcc gcctatcagc agcagtcggc ggcgcagatg
    20341 ccggatttgt ccgctctgct ggcgcgggcc gagcgcaagc tgtggcaccg tgacgcgcag
    20401 tgcgacgtca aaattggcga ctatgtgtgg agcaatttcc gtgagcgcca tctgtttctg
    20461 gcgtacgccc atgtccgcgc cgaagcgatt ggtgagttgg cgcagcggct gtggcgcgcc
    20521 gtgcagccgc tgctgggcgg tgatgccgac gctgcctggc ggcgcttgaa cgccgccatc
    20581 gacgccatgc ctgatatgga taccatggaa gagccgatcg acccgctggt cgcccaagag
    20641 ctggggttga aattctacca gccggacatg cgctttcgct ggtttgacca gcgctggacc
    20701 tttgccgagt acatcacccg ctatatcgac tacgacacct cctggtaagc cgtgctcgcc
    20761 atgctcttct ggcggtgcgc tcaggcgcgg cggcgcagca ccaccacatc ctgatggccg
    20821 gccatcgcct cgatgatgtc cagcacgtca aaataccgcc cccagtgcgc caggatgtaa
    20881 tccggggcat gcagcacatc cgggccgctg ggcgtggggc tgtccagccc ggcatggccg
    20941 gccatatcat gccagccggt gcggtgggct tccagcttgt gcaccatggg tgtgcgatag
    21001 agcgccatat gggtcagccc ctgcactgac agcaacagca gtgcgccggg gcgggtgagg
    21061 cgttgcagct cggccagcca gctgtcctgc tcggcttcgc tcaggtgcgg cagcatcgac
    21121 aggcccaata ccagatcgaa ttgcccgctg gccagcgtgt ctcccatggc gacaaagtgg
    21181 gctgctggca gggcggtgcg gcaatactgc atatgcgccg gctggctgcc catgccggtc
    21241 acggccgggc cctgccctac cagatagcgg gtcagccggc ccacgccaca gccccaatcc
    21301 agaatcgcgg taaacgatga caaggggcga ttaaagcgtt cgctcagcag atgggcaaag
    21361 cgcgccacca tggtggcgcc gcccagacaa aaagcctgag ggtctggcgt gccgatcacc
    21421 tgggcaatct gttctgccgg cggtaagggg gattcctggg cgggatccgc cagaaaccag
    21481 gcggtgcggt aagaccaggc atgttcgcca gcggggccgg tgacattgaa gcgtgcccag
    21541 ccgccaggaa agctcagtcc gccgtccgtc agataatgcc ggcaacgaaa gcgtgaatgg
    21601 gctgcctgtg gcacgtcggg aaacactgtc agcaaatcgg gcgagggcaa cggccattcc
    21661 agtacgtcaa aatccacgcc attgaccaaa aagcggcact gcgtctgcgc atcccacacc
    21721 ccgagcgccc agccttccat cagcagactg tcagcgtcca gcgtgatgcg atccggcatc
    21781 cagcgcagat gcctggctac ggccaactgc aaatcggcct cgtaaagcgt catgatgaaa
    21841 atccaggcag aggaatggcc gcattatggc atgcgctggg tgtcagccgc gcatgacggc
    21901 agtaccggct gaaggggtgg ctcgttcctg tcgttcacaa catctgcctg acgttgctgt
    21961 ttcctgtcct gtctgcggcc acaccgggcc aggagcgctg cgctgggttg ggtgagcgac
    22021 tcttagggtg tgccgctgtg cgctgtggac gaagcttctg ccgccatgac cgctacccga
    22081 ttacggccat cggccttggc ctgatagagc gcgcggtcgg cacgttgcag cagactttcc
    22141 agtccttcgt cattcgcgtc cagcatggcc acgccaatgc tgacagtgaa tgataccggc
    22201 gcatcgccat cgttgagcgc gcgcacgcag ctggcggcca ggacgcgcac gcgctcggcc
    22261 agggtttggg cgccggccag gtcggtttcc ggcaggatgg cggcaaattc ttcgcccccc
    22321 aggcggccgc cgtgatcttg ctggcgaatg gtgtcgcaca gcagccgcgc catcacctga
    22381 atcacccggt cacccgtggc gtggccccag cggtcgttga tgtgcttgaa gtgatcaata
    22441 tccaccatca gcaccgacaa gggccgctgg tagcgccgtg cgcgcgccac ttcggcggcg
    22501 ccggtttcca tcagttgccg gcgattggcc acgccggtga gctcgtcggt gatggccagc
    22561 cggcgcatgg cctcgctgtg gcgcagcacg gtgaggtggg cgcgcaggac cagcacggcc
    22621 aggatgccca gcacggcaaa gccaatcaaa aactgccagc cacggtgcca ccagccggtc
    22681 agaatgcggc ttttgtcgaa acccaccatg gcgataaacg gaaagcgttc cagtcggctc
    22741 aggccaaaga tgcgctcgcg atggtcaagg ggtgacgtgg cccagaacat ggtggtgcgt
    22801 tccatctgga caaaaggcgg atagccgggc ggcggcgagg tgggtttacc catggcggcg
    22861 ggcagcggcg ggttgcgtgc cagtacgatg ccatcgttgt caacaatggc cattaaatca
    22921 ttgggttcca gcgagaaaga ttcaatccag cgctgggcat ggcccagatc catcaccgcc
    22981 atcactccgc ccagcaggcg gccttgatca tcgctgaggg tgcgcgacat cagcaccacc
    23041 ggctgaccac tggccgagcg ttcggccggc atgtactgaa tgtgcagcgg ttcgctgaaa
    23101 ttgacctgcc tactgtcgca aaagcgctgc tggctacgaa agcccagtaa ttgcggccgc
    23161 gatgccacgg cggtaaacac gcagttgtgg tcaaacaaca ccaggtcttc caggccggat
    23221 accgtggtca gtttttcgtg caacagcttg tgcaattggc gctggcgggc cttggcgtga
    23281 tctgcggacg ggtttaaatc ctgcgtgggg tcgatccggc cggcaatatc gcgcaagaca
    23341 taatctgccg acaaaaaagc atcgccaaac acactgctga gcaactggct tttatgcagc
    23401 gccagccggc ccatttcatc cagcgtgcgt gcccgttcgt gcagaaaatc cagcgccagc
    23461 cagctgccga acaggcccag aaacaccagc gtgccggcgt acagcgtcca gcggcggcgt
    23521 gttgagtgag caaggtcgga agaaaaaatt gccatgatgc caactggggg tgagtgcgga
    23581 gcaagtcaga aatttgtcat gaacagtgta ctgtgccagc cgggggattt cgagcacagc
    23641 attttgttca catatgaaaa accccgccgg gtggcggggt tgtggcagat gccagacagc
    23701 agacagatca gttcttggcc tgatccacca gcttgttctt ggcgatccac ggcatcatgg
    23761 cgcgcagctg ggcaccgacc ttttcgatct ggtgatcggc ggtcaggcgg cggcgagcag
    23821 tcatgctcgg gtagttggtc ttgccttcca ggatgaacat cttggcgtat tcgccggact
    23881 ggatgcgata cagggctttc ttcatcgctt ccttggtggc ggcggtcacc acttccgggc
    23941 cggtcacgta ttcgccgtat tcggcgttgt tggagatcga gtagttcatg ttggcgatgc
    24001 cgccttcgta catgaggtcc acgatcagct tcagttcgtg caggcactcg aagtaggcca
    24061 tttccggggc gtagccggct tcaaccaggg tttcaaaacc agccttgacc agttccacgg
    24121 cgccgccgca cagcacggct tgttcgccga acaggtcggt ttcggtttct tcgcggaagt
    24181 tggtttcgat cacgcc
//
LOCUS       DAOPYX010000006        23392 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_2187_length_23392_cov_8.587393, whole genome shotgun sequence.
ACCESSION   DAOPYX010000006 DAOPYX010000000
VERSION     DAOPYX010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 23392)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23392)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23392
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2187_length_23392_cov_8.587393"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(231..914)
                     /locus_tag="PKN55_00675"
     CDS             complement(231..914)
                     /locus_tag="PKN55_00675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX69787.1"
                     /translation="MIIRSALLAVCCLPLLAHAAAPADGVYVTERGWGVLSVKAGAFK
                     LDTMGSNAHTCQVEGKLNAQGVSRPDTVGSDERCELRLSREGAGVNVASTPGCRYFCG
                     MRAGLDGLYLKPAAGCEPAQLRRQRALFKQQYDRKDYTGAVATLAPLLSQCQRTLDWL
                     GEPWLRNDLALAQLRAGDAAACRATLAPLAADAARSDEAIRNDYPPSDADAYLSVLKA
                     TRTNLKLCR"
     gene            complement(1038..1895)
                     /locus_tag="PKN55_00680"
     CDS             complement(1038..1895)
                     /locus_tag="PKN55_00680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011135920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HNX69788.1"
                     /translation="MSHTLFEGSDHYVATADLMMAVNAAITLQRPLLVKGEPGTGKTM
                     LAEEIARALGRELIVWPIKSTTKAQQGLYEYDAVSRLRDAQLGEARSQEVGHYIIKGK
                     LWEAFDAEQAPVLLIDEIDKADIEFPNDLLRELDQMEFYVYETRQQVRARQRPIILIT
                     SNNEKELPDAFLRRCFFHYIRFPDADTLRQIVQVHYPDLREQLIGEALEVFLAVRELP
                     GLKKKPSTSELLDWLRLLADERLTPGEAPLAASLSGASAPPHAGALLKNEQDQHLLER
                     LIGLVKNRR"
     gene            complement(1892..2308)
                     /locus_tag="PKN55_00685"
     CDS             complement(1892..2308)
                     /locus_tag="PKN55_00685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008952812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX69789.1"
                     /translation="MKRYAPNSPQAIARILCMFMMGDGVMDPRELDSFDRLDLLSLLG
                     LERKAFLALFHDYCNDISDEAEPDGSIHLVDRARADLVLDEVTDHQKRLLTCAIALDL
                     CKADDTISEPEMALLQYMMAKWHITLDTLEEQFIRQ"
     gene            complement(2305..2766)
                     /locus_tag="PKN55_00690"
     CDS             complement(2305..2766)
                     /locus_tag="PKN55_00690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008952811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HNX69790.1"
                     /translation="MARVKLTLPDAWQFDTTVTVRIGDINYGGHLGNDAVLSLVHEAR
                     LQYFKSLGYSEIDIEGVGIVLADAVVVYRAEAFHGDALRVRVGLGDFNAYGFDMFYHL
                     ENPAADKEIARVKTGIVFFDYQTRKIAPIPAAFIQRAGLAELVAAAAKESA"
     gene            2983..3462
                     /locus_tag="PKN55_00695"
     CDS             2983..3462
                     /locus_tag="PKN55_00695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018747782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNX69791.1"
                     /translation="MYQRIFVPVDDSSTSRRALDEAIKLAQHTGATLLLAHVVDLAQF
                     GWGGTEFLDASELQKNIKATGEAVLNQAREAVEAAGLPCSAVLLESWGDKMAEVLVGE
                     AEKEQAELIVMGTHGFSGLMHLLMGSVAEGIMRRANVPVLLVRSDETPDAGAPPAVM"
     gene            complement(3766..4083)
                     /locus_tag="PKN55_00700"
     CDS             complement(3766..4083)
                     /locus_tag="PKN55_00700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018149776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HNX69792.1"
                     /translation="MKLFEKIPNPREIRRKLGLNQQEFWSKIGVTQSGGSRYESGRNM
                     PKPVRELLRLVHIEQVDLSKVKREDFEIIEYLKDTHPDLYKSLRKAVKAQAEMQTDPV
                     AQA"
     gene            complement(4338..4436)
                     /gene="ffs"
                     /locus_tag="PKN55_00705"
     ncRNA           complement(4338..4436)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PKN55_00705"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(4546..5112)
                     /gene="msrA"
                     /locus_tag="PKN55_00710"
     CDS             complement(4546..5112)
                     /gene="msrA"
                     /locus_tag="PKN55_00710"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014086951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HNX69793.1"
                     /translation="MTALEQTPLATATLAGGCFWCLEAVFTRLRGVHDVRSGYTGGYT
                     PNPTYAEVCEGHSGHAEAVRITFDENLIAFSTLLEVFFAVHDPTTLNRQGHDIGTQYR
                     SAIFCHDARQRAQAESVMAELAHACHWPDPIVTQVVDAGVFYPAEMAHYRYFDQHPGQ
                     PYCRAVVGPKVAKLQQYFIHLVKETSGT"
     gene            complement(5210..6169)
                     /locus_tag="PKN55_00715"
     CDS             complement(5210..6169)
                     /locus_tag="PKN55_00715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008953997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HNX69794.1"
                     /translation="MRTRKEADCTRQPDKIPTTIMQIFHIDQFVLPLPAGHRFPMEKY
                     AMLRHAVAAFAGELLCVPDAAHADELVLAHDPDYITQVFSGKLPAAAQREIGFPWSPG
                     LVERSRRSVGATIAACRSALWSGCGVNLAGGTHHAAYARGSGFCVFNDIVTAVRVMQR
                     EGRIRRALVVDLDVHQGNGTAALCEGDASIYTFSMHGAGNFPFRKHPGDWDIEFPDGT
                     GDEPYLAALASALPALFTRARPDLLVYLAGADPLASDRLGKLNLSQAGLAARDAMVLD
                     ACDAQGMPVALCMGGGYAQPISDTVAVQTETVRETVRRYAGAR"
     gene            6205..7170
                     /locus_tag="PKN55_00720"
     CDS             6205..7170
                     /locus_tag="PKN55_00720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003742203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HNX69795.1"
                     /translation="MRILLAPMQGLVDAPMRALLTSMGGVDACVTEFVRVTHTLLPAR
                     TFYRLCPELHHGGCTPSGVPVSVQLLGSDPACLADNAARAAELGAPAVDLNFGCPAPT
                     VNRHRGGAVLLREPELLHAIVRAVRAATPAQVPVTAKMRLGYDDTALALDCAQALATA
                     GAAEIVVHARTKTEGYRPPAHWDWIARIREAVTVNVVANGEIWTLQDAQRCLEISGSQ
                     DLMLGRGLVARPDLAGQIASWASGRPEVVLDWPGMRLVVTQFWGLLQAYADGAYPQAR
                     LKQWLALVAQAFPAARPLLESVRRQPDSHSVAACLADDTYWQVGR"
     gene            7283..7771
                     /locus_tag="PKN55_00725"
     CDS             7283..7771
                     /locus_tag="PKN55_00725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014086435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HNX69796.1"
                     /translation="MSTANQGILLVCMGNICRSPTAEAILRAQLRARGLEHDVQVDSA
                     GTHAYHTGEAPDARARQAAQAAGYALDGMRARQVLADDFARFDWILAADAQNLAELRR
                     RCPAQWQDKLRLMRDWAGGGDVPDPYYGGAQGFTEMVAMLETALAAALDAWYPPATGG
                     KP"
     gene            7768..10569
                     /locus_tag="PKN55_00730"
     CDS             7768..10569
                     /locus_tag="PKN55_00730"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNX69797.1"
                     /translation="MTSRELAHATELPDWQQAVGDVVFDWWVAARRLECSTQLCTLLG
                     LSRMPADPLAELNQRIHPDDLSALQLALNACWHSADGWLRHECRVRGGDGQERWMLIR
                     GQLLERDGLGAPRHVRGVLSDISARKADEAMLRRYEHIVATSSDAMYFVDADCRIVLA
                     NPAMTIRFGLTPEQVVGRPMATVFGLVVFDELSHDLARALAGEAMHFQRWMHYPALGR
                     RFVDVHYDPCGQAGSPAHGVVVTVRDLTDSARQALLLAQTQRAAHIGGWEIDCQRQEV
                     FWTEEMFCLLGVPAGLPAQTFQQWSSRMPAAQWAQLSAGLADVTAGVRAEFALVVEVG
                     GQDGRTQALALQARGERNSRGEVIRVFGSLQDVTARRQAQAQLRLAASVFESSREAVF
                     ITDAERRMLTVNPAFCTLTGLDAVAVVGQSVRCLSSPRHDAMFYRDIWRTAAREGGWH
                     GEVWGVNAEGEDYPQWASLTAVRENDGAISHYIGMFADISARKSAEARVQQLVNFDSL
                     TDLPSRALLQQRLPDMLAGHRYADSILGVLFIDLDRFKNIADTFGHRASDEILRQVAT
                     RLQGCIGREDLLCRHGGDEFVLVLANVDHATELARRASLVREALARPMWLDGTELVLT
                     ASIGISVFPQDGDNADELLRRADAALHHAKAEGRDNVQFFTESLNARAAEFLQLEHSL
                     RRALGRHEFRLHYQPQIDMRTGQLVGMEALVRWQHPSLGLVAPSKFIPVAEDTGMIVA
                     IGEWVMLEACREAQRWRELGLADVPVAVNLSANQFRAGVRDCVARALAYSGLPANRLE
                     LEVTESLVMGAADQVIASLRELKEMGLILSIDDFGTGYSSLSYLKRFPIDRLKIDRSF
                     VCDITTNPDDAAITTAIIHLGHNLRLHVVAEGVETRQQWDFLREQGCDQAQGYWISKP
                     LDSEALLEFLRSWQGGLSS"
     gene            10566..11258
                     /locus_tag="PKN55_00735"
     CDS             10566..11258
                     /locus_tag="PKN55_00735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011136686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HNX69798.1"
                     /translation="MTASAPRLPASFTLTPSHTADVVLPFTPGERRFGSSPVVVIGAS
                     TGGTEALKTVLCQLPDNMPPIVVVQHMPEMFTRSFANRLDSLARLHVKEAEQGDVLKP
                     GCAYIAPGHSHVLLSPSGNTLTLRLSDAPEVNRHRPSVDVLFRSAANVLGRSAIGVIL
                     TGMGRDGARGMAEMKQCGAYNLAQDEASCVVFGMPKEAIALGGAHEVLPLNQIAARLT
                     SLCEKRIHERQH"
     gene            11317..12771
                     /locus_tag="PKN55_00740"
     CDS             11317..12771
                     /locus_tag="PKN55_00740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011136685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNX69799.1"
                     /translation="MRNLAHNALPVLVVEDDADLREAIVDTLNLAGFSTLEARDGQSA
                     LACLKQELVGLVLSDAQMQPMDGYQLFAEIRQRYPGIPFILMTAYGVIERAIELLRNG
                     ASHYLLKPFEPDVLTAEVEKHILKPRCNDSHELIAHDPSMQDVLRLASRVAVTDATVL
                     LNGESGVGKEVVARFLHRHSMRADAPFVAVNCAAIPENLLESTLFGHEKGAFTGASSA
                     FAGKFEQAQGGTLLLDEVSEMPLTLQAKLLRVLQERELERVGGSRTVKLDVRVICTTN
                     RNLAEEVKKGAFREDLFFRVNVFPLAIPALRQRKGDIVPLARHFLEKYAPTHKRQSAP
                     GLSPAAIEDLTAYRWDGNIRELENVIQRALILSSGDSILPQDLMLPSDSQPAEPVITP
                     PPRQDHAAHAAADDDASMYVLEKRHILDTLAAVNGVRKLAAEKLGISERTLRYKLQRY
                     REEETAPAGAQEQHTAEVLPTHQMSGEQHVCTRH"
     gene            12752..13075
                     /gene="fliE"
                     /locus_tag="PKN55_00745"
     CDS             12752..13075
                     /gene="fliE"
                     /locus_tag="PKN55_00745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HNX69800.1"
                     /translation="MSVQGIDQVLGEMRGVSALAGGRAPEPVVSTPPSVDFAEVLRSS
                     IEQVNTVQQTSQDLQKRFELGDESVNLHDVMISLQKASLSFQTMVQVRNKLVTAYQEI
                     MNTQV"
     gene            13176..14858
                     /gene="fliF"
                     /locus_tag="PKN55_00750"
     CDS             13176..14858
                     /gene="fliF"
                     /locus_tag="PKN55_00750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014086438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HNX69801.1"
                     /translation="MAEIAEQGNNLKQNRVRESMERFRDLPTNKKSLLYVGIAAVAAL
                     VIGTALWMREPTWKVLFSNVSDKDGGQITTALQQMNVPYELSSGGTIAVPAEKVYETR
                     LKLATQGLPKASGVGFELMDNQKFGISQFAEQVNYQRSIEGELARTIQALSAVESARV
                     HLAIPKNSVFLREQQKPTASVVLNLNSGRLIDPTQIHGIMNLVASAVPGLPPRNITIV
                     DQEGNMLSTMGELNQQNQNGLDQRQLNYVKEIEQTYIKRIETILEPIFGKGNARAEVT
                     AELDFSQREQTSENYTPNSPPNAAAIRSQQMHESTEREPLGPGGVPGALSNQPPNAAA
                     APITLPAGTQAGVSTTGQATPDKSTKKDSTVNYEVDRTIQHVKMPVGMIKRVSAAVVV
                     NYKKKVDNNGESKFVPLTQQELGQINNLVREAMGYNPARGDTLNVVNAAFADNTARPA
                     LEQRAIDFITENIFDIAKWGLLLLVLLYLIFGIIRPIVREVINPPVPEEEYPDGPAIE
                     GGEGEGEEGDEVVDEVEMQRREYAANLEAAREIIRNDPRMAAQIIKEWISDE"
     gene            14851..15849
                     /gene="fliG"
                     /locus_tag="PKN55_00755"
     CDS             14851..15849
                     /gene="fliG"
                     /locus_tag="PKN55_00755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009433 - bacterial-type flagellum basal
                     body, C ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HNX69802.1"
                     /translation="MSDAGVRRSAVLLFSIGQQDAVEVFKYLGPKEVQKISTNMTQIT
                     NLSIDEIATSIQLFRDEAKMRASIGAADEYLRQVLVEALGEDKASNLLDKIFAGNDNS
                     GIESLKWMDPSSAADLIRNEHPQIISTILVHLEPDQSSAIMQHFSERLRNDVLLRIAT
                     LEGVQPQAIRELNDVLTQLLSGSDRVKKSAIGGVQMTAEILNFMGSSYEASALGSIRE
                     YDPELAQRIQDKMFVFENLCDLDNRSIQVILREISPESLITALKGTSQELKDKIFKNM
                     SSRAAEMLKDDFEAKGPVKVSEVEAEQKEILKIVRKLADDGQIVLTGKSGEEGVVE"
     gene            15839..16795
                     /locus_tag="PKN55_00760"
     CDS             15839..16795
                     /locus_tag="PKN55_00760"
                     /inference="COORDINATES: protein motif:HMM:NF014196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HNX69803.1"
                     /translation="MSSKIIPAGELQGQALPTLQFASFGQITPNSRLARVAAAVTPKV
                     TLPASVDLGALLAAAGVDDPTAQAVDSSFAADPVEAINDSLPPSMQLDADEASALLAP
                     VEGFDESGTGEEIALQEDSEPEPEPEPAPQWPTAAELEAIHQEAYQAGYDAGHAEGLA
                     RGADAGHQQAFAHASQQFAQELQAFADLQASFQANLTELEAEMAPALLNLAVNAMRKL
                     LHDHLACKPDAVLGVVRQALHSIPGEVARATVRAHPNDLQALKTFLPANTPDTQWQFV
                     EDASLMSGGCLIELPSASLDLTLQTRWKALMATLGADGDVSA"
     gene            16782..18170
                     /gene="fliI"
                     /locus_tag="PKN55_00765"
     CDS             16782..18170
                     /gene="fliI"
                     /locus_tag="PKN55_00765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011136681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HNX69804.1"
                     /translation="MSALERWLAYVQACGHTLEEAALWQPKGRLTRVTGMVMEAVGLR
                     LPVGSACVIEIDEQHHVEAEVVGFSGECLYLMPVTNVHGLVPGTPVSALISHHPPQYE
                     HHGVPQNANGPFGRQVPVGASLLGRILDSLGRPLDGQGPIHPDAYYPLYAQPINPLDR
                     TPVRDVLDVGVKAINGLLTVGRGQRLGLFAGSGVGKSVLLGMMARFTKADVVVVGLIG
                     ERGREVKDFIENILGEDGLARSVVVAAPADSPPLMRLHGAAYATALAEYFRDQGLSVL
                     LLMDSVTRYAMAQREIALAIGEPPVTKGYPPSVFAKLPQLIERAGCGDNGGAITGFYT
                     VLSEGDDQQDPIADSARAILDGHFVLSRHLAEAGHYPAIDIEQSISRVMVDVVSKEQL
                     TTARNFKQLYSRYQRNRDLINVGAYVSGSDRMLDEAIRLHPTMEAFLMQAMDESFDLD
                     DTRQLLDSVLVS"
     gene            18242..18688
                     /gene="fliJ"
                     /locus_tag="PKN55_00770"
     CDS             18242..18688
                     /gene="fliJ"
                     /locus_tag="PKN55_00770"
                     /inference="COORDINATES: protein motif:HMM:TIGR02473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HNX69805.1"
                     /translation="MPVSKYELLIRLAAEKCDDAAHAMNAARQRMETARQRLQQLAQF
                     LADYLHRRIARGEQGMSSGQWVDYQQFIERLQEAIDLQRREVDSSQIAYDKATAAWQE
                     ARKKLKALEKLQEQEELRARLREAKREQKQTDEFAARIFRESRSRI"
     gene            18742..>19449
                     /locus_tag="PKN55_00775"
     CDS             18742..>19449
                     /locus_tag="PKN55_00775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX69806.1"
                     /translation="MLDHAKTLSRGAPAMTIKVTSQSTAANAATSLFRQPGAQGDTSD
                     LFASLLAGLGDTLLTDPKTGLLGDDAALSPLARKLKAAKAGDQPQQSQSDLNAMLGAA
                     ALLPQVQQALAQQLNTQKTVTGQEGESRAGTGTPGVDPALLAAIATHGRLNAQAGTEQ
                     GNTSDGKPQAQPGMQALANALGQKQGEGKPAEVQASNQTAAPAEVAATGQKTRPEAAD
                     FTQTLREAAAQAAADTPA"
     assembly_gap    19450..19549
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <19550..20249
                     /locus_tag="PKN55_00780"
     CDS             <19550..20249
                     /locus_tag="PKN55_00780"
                     /inference="COORDINATES: protein motif:HMM:NF014207.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HNX69807.1"
                     /translation="FTQTLREAAAQAAADTPAVAATGQKTRPEAADFTQTLREAAAQA
                     AADTPAPVAADGRQIQVAVPSAQRGAPQHVVSIPTPVGQPQWTDDVGQQVVVMMNAKL
                     ETAQLQVTPPDMGPVEISLKIGSDGAAQVSFVAGVAETRQALEQGMPRLSAMLADNGI
                     RLADAQVSSGQGQAFRDNTQFAQQGQQGGGQSGHGQQSGHVPGSQDQAVLMNLPDSAI
                     TSQSVSNNEVSIYA"
     gene            20460..20960
                     /locus_tag="PKN55_00785"
     CDS             20460..20960
                     /locus_tag="PKN55_00785"
                     /inference="COORDINATES: protein motif:HMM:NF015693.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HNX69808.1"
                     /translation="MAQSKATPVAPNQKTPGNKRMLMIGVALLVALLLLLAGAVGYLF
                     LNKNKEHGDEPTLGAPGTLPVFEKMEPFVINLSGEQGGMLQVEMETELAAESSREIVK
                     AYNPKVRSAIIMLLSSKTSQELATAEGKAKLRNQIRKIINETIGASPDRPLVVGVVFT
                     SFIVQQ"
     gene            20973..22001
                     /gene="fliM"
                     /locus_tag="PKN55_00790"
     CDS             20973..22001
                     /gene="fliM"
                     /locus_tag="PKN55_00790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014086444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HNX69809.1"
                     /translation="MADDILSQDEVDALLRGVSGTSEADDAHIAPVRPYDIGRQERIV
                     RGRMPTLEIINERFARNLRIGLFNFIRRNAEISVGPVRVQKYSEFIRNLVVPTNLNLV
                     HVKPLRGTGLLIFDPDLVFLVVDNMFGSDGRYHVRVEGRDFTPTEQRIIRRMLDVVFT
                     EYQKAWEPVYPIEFVFMRSEMNTQFANIATPTEVVVAVTFRIELGAGGGDFHVCMPYS
                     MVEPLRDLLASTMAADRTEVDNRWVNLLSKQVQVAEVDLVATLAEAQITLGQILNLKK
                     GDVISVEIPETINASVSGIPVAECHYGTINNQYALKVEKILAGTQEMSELAIPRDFRL
                     NKPNLSGQ"
     gene            22028..22459
                     /gene="fliN"
                     /locus_tag="PKN55_00795"
     CDS             22028..22459
                     /gene="fliN"
                     /locus_tag="PKN55_00795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018749091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HNX69810.1"
                     /translation="MDDWAAAMAEQASATEAAAPASAPAPAASSTPQPAINLFQDFAG
                     TPMDMSAGGDAAAQNLDMILDIPVNMTVELGRTKIAIRNLLQLAQGSVVELDGLAGEP
                     MDVLVNGCLIAQGEVVVVNDKFGIRLTDIITPSERLRRLQK"
     gene            22474..22935
                     /gene="fliO"
                     /locus_tag="PKN55_00800"
     CDS             22474..22935
                     /gene="fliO"
                     /locus_tag="PKN55_00800"
                     /inference="COORDINATES: protein motif:HMM:NF016250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HNX69811.1"
                     /translation="MRRTLFLALPCWAALAFPGLALATTVAPAATSVELSPVSTLLQT
                     VLAMAVVLGVILGLAWLARRVNPMALTGNPASLRVVSGVMVGQRERVVVVEVQGKWLV
                     LGVAAQSVNLLCTLDKPEDVPADVPVARGAFADKLRTLLESRGISLPKKPS"
     gene            22932..>23392
                     /locus_tag="PKN55_00805"
     CDS             22932..>23392
                     /locus_tag="PKN55_00805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011136674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX69812.1"
                     /translation="MKLHSFISGLHTRLSRPGFWLVLALSVVGMLVSGVLWMEPAMAE
                     GLPMMNSTPAPGGGNNYSLSLQMLLFLTSLSFIPTALLMMTSFTRVVIVLSLLRQALG
                     TMQSPPNQVIVGLSLFLTLFIMGPVLDKVYKEAWVPFSEDKINFLQALDKGS"
BASE COUNT         4721 a         6798 c         7098 g         4675 t
ORIGIN      
        1 tgaccagggt gacgttggcg cagaccggac atttcatggt tgttttccgt gtgtggtgaa
       61 tgccaaatgg actgtcagcg caagcgaagg ttcatgcccg cgctgacagt cctggcttag
      121 agtcgctaac aaaacccagc gaagcggccc tggctaggcg tgtgaccgca gacagtacaa
      181 gcagtacggc aaggtcacac aacaacgcca gggggttttg tgagcggctc ttaacggcac
      241 agcttgaggt tggtgcgcgt ggctttcagc acgcttaagt aagcgtcggc gtcgctgggt
      301 gggtagtcat tgcggatggc ctcgtcgctg cgcgcggcgt cggcggccag cggcgccagg
      361 gtggcgcggc aggcggcggc gtcgccggcg cgcaactgcg ccagcgccag atcgttgcgc
      421 aaccagggct cacccagcca gtccagcgtg cgctggcatt ggctgagcag cggggccagg
      481 gtggccactg cgccggtgta gtccttgcgg tcgtattgct gcttgaacag cgcgcgctgg
      541 cggcgcagct gtgcgggctc gcagccggcg gcgggcttca ggtacagccc atccagtcca
      601 gcgcgcatgc cgcaaaaata gcggcagcca ggcgtgctgg ccacgttcac ccctgcgccc
      661 tcacgactca ggcgcagctc gcagcgctcg tcactgccga ccgtatctgg ccgtgacacg
      721 ccctgggcgt tcagcttgcc ttccacctgg caggtatgtg cattgctgcc catcgtatcc
      781 agtttgaacg cgccggcctt gacgctgagt acgccccagc cgcgttcggt gacatacacg
      841 ccatctgccg gcgcggcggc gtgcgccagc agcggcaaac agcagacggc aagcaaggcg
      901 gaacggatga tcatggcggc tctcctgata ttcggcaggc aatagtgtag caggctctcg
      961 tggggtcgcc ggccagcaaa atggcccacc gacggctgtc tggtgggcca ttttgctggc
     1021 gaaatcgagc caaccgatca gcggcggttt ttcaccaggc caatcaagcg ctccagcaaa
     1081 tgctggtctt gctcgttttt cagcagcgcg ccggcatgcg gcggcgcgct ggcgccgctc
     1141 aggctggcgg ccagcggcgc ttcgcctggc gtcagccgtt cgtcggccag caggcgcagc
     1201 cagtccagca gctcggaggt ggagggtttt ttcttcaggc cgggcaactc gcgcacggcc
     1261 agaaacacct ccagcgcctc gccaatcagc tgctcgcgca ggtcggggta gtgcacttgc
     1321 acgatctggc gcagggtgtc ggcgtcggga aagcggatgt aatgaaaaaa acagcggcgc
     1381 aaaaaggcgt cgggcagttc tttttcgttg ttgctggtga tcaggatgat tggccgctga
     1441 cgggcgcgca cctgctggcg ggtttcgtag acgtaaaatt ccatctggtc gagttcgcgc
     1501 aacaggtcgt tgggaaactc gatgtccgcc ttgtcgattt cgtcgatcag cagcaccggc
     1561 gcctgttcgg cgtcaaacgc ctcccacagc ttgcccttga tgatgtagtg gcccacctcc
     1621 tggctgcgcg cctcgcccag ttgggcgtcg cgcagccgcg agacggcgtc gtactcatac
     1681 aggccttgct gcgccttggt ggtggacttg attggccaga caatcagctc acggccaagc
     1741 gcacgggcga tttcctcggc cagcatggtt ttgccggtgc caggctcgcc cttgaccagc
     1801 agcgggcgtt gcagggtgat ggcggcgttc accgccatca tcaggtcggc ggtggcgacg
     1861 tagtggtcgc tgccttcaaa cagggtgtgg ctcattggcg gatgaactgt tcttccaggg
     1921 tgtccagcgt gatgtgccat ttggccatca tgtattgcag cagggccatt tccggctcgc
     1981 tgatggtgtc gtcggccttg cacaggtcca gcgcaatcgc gcaggtgagc aggcgctttt
     2041 ggtggtcggt gacttcatcc agcaccagat cggcgcgggc gcggtccacc aggtggatgc
     2101 tgccgtccgg ctcggcttcg tcgctgatgt cgttgcagta gtcgtggaac agggccagaa
     2161 aggccttgcg ctccagaccc agcagggaga gcagatccag gcggtcaaag ctgtcgagct
     2221 cgcgcgggtc catcacaccg tcgcccatca tgaacatgca caggatgcgg gcaatcgcct
     2281 gcgggctatt gggggcgtaa cgtttcatgc ggactccttt gcagctgcgg cgacaagctc
     2341 ggcgagcccg gcgcgttgga tgaacgccgc cggaatcggg gcgatcttgc gggtttgata
     2401 atcaaaaaac acgatgccgg ttttgacgcg ggcgatttct ttgtcggcgg cggggttttc
     2461 caggtggtaa aacatgtcaa agccgtaggc gttgaagtcg cccaggccca cgcgcacgcg
     2521 cagggcgtcg ccatgaaagg cctcggcgcg gtacaccacc acggcgtcgg ccagtacaat
     2581 gccgacgcct tcgatgtcga tttcgctgta acccagcgat ttgaaatatt gcagacgggc
     2641 ttcgtgcacc agcgacaaca cggcgtcgtt gccaagatgg ccgccgtagt tgatgtcgcc
     2701 gatgcgcacg gtgacggtgg tgtcgaattg ccaggcgtcc ggcagggtca gtttgacgcg
     2761 ggccaaggcg cgctccattt ctattacaga atcaaaacag catcgagtgt atgcgtgggc
     2821 ttgtgctaaa tcaacagcgc gctaactggc tttgctagac tgaactcaac tgtgcggctt
     2881 tgggtccgat tctggcgccc gctggcggcg caggccggcg cagacgggcg ggaatatcgc
     2941 ttggtctggc gcgcggcatt tgccaatttc aaggagcatg tcatgtatca acggattttt
     3001 gtcccggtgg acgatagcag tacttcgcgc cgcgcgctgg atgaggccat caagctggcc
     3061 cagcacaccg gggcaacgct gctgctggcc catgtggttg atctggccca gtttggctgg
     3121 ggtggcacgg agtttctgga tgcttccgag ttgcagaaga atatcaaggc taccggcgag
     3181 gcggtgctga atcaggcacg cgaagccgtt gaggccgctg gcttgccatg cagcgccgtg
     3241 ctgctggaaa gttggggcga caaaatggcc gaggtattgg tcggtgaagc agaaaaagaa
     3301 caggccgaac tgatcgtgat gggcacccat ggcttttcgg gcttgatgca cctgctgatg
     3361 ggcagtgtgg ccgaaggtat catgcgccgc gccaatgtgc cggtgttgct ggtgcgctcg
     3421 gatgagacgc cggatgcagg cgcgccgccg gcggtgatgt aagggcgaag ataaacaccc
     3481 ccgttgcgca tcaggctgct ggccgatgcg caacgggggt gtttttttgg ctggcgccaa
     3541 aagcatcacg ccatccgttc gctgccaggc gggggatggc gtgatgggtg cagctgtcgg
     3601 gcaacgggat ggtttatcat ggggccaacc atgacaaacc atccagcgcc gtgaagacag
     3661 tcgcaacaag cggtggttca cggcgacgca gctcggcatg agaatcatgc cgcttgcccc
     3721 tgcgtggcgc gcaagcgtgc cgcttgcagt gaggattttt caggctcagg cctgggcaac
     3781 cgggtcggtt tgcatttcag cctgggcttt gacagctttg cgcaggctct tgtacaaatc
     3841 cgggtgggtg tccttgaggt actcgatgat ttcaaaatct tcgcgcttca ccttggacag
     3901 atccacttgc tcgatatgca ccagacgcag caattcacgc accggtttgg gcatgttgcg
     3961 accggattca tagcgggagc cgccggattg ggtgacgcca atcttgctcc agaattcttg
     4021 ttggttcagg ccgagcttgc ggcggatttc ccgtgggtta gggatttttt caaacagttt
     4081 cataacgttt cctctcgtcg taatggcagc gtacgccccg cactaaggtt ataagtcaaa
     4141 tctggaaaaa ttccaaacac atagtgtgtg attgaccatg cattgaggca aaagttctac
     4201 gtacttccac gtagtatttt accgtgcctt gtgcggacga caacggaatg gcgaaataaa
     4261 gttgggtaaa aaccacaacg cgccaatctg ggccatcgca gtgatgatga gaggggtggt
     4321 aaattcagag gggaaggggt ggcgagcctg acccccggca cttgcaataa gcggctgtgg
     4381 ctgcttcctt ccggacctga ccaggttcac caccttgcaa tgcggggaga cccgccatag
     4441 aacttcacat cttacccaat atcatcacac aaggcaagcc tgcatgtatt gcctgttacc
     4501 aagcctgcgc acggtatcgg aaccgcaggc cagtaagggc cacaatcatg tgccggatgt
     4561 ttcctttacc aggtgaataa aatactgttg taatttcgca acttttggtc ccaccaccgc
     4621 gcggcaatac ggctgccccg gatgttggtc aaagtagcgg tagtgcgcca tctctgccgg
     4681 ataaaacacc cccgcatcca ccacttgcgt gacgatcggg tctggccagt ggcaggcgtg
     4741 tgccagctcg gccatgacgc tttctgcttg ggcgcgctgc cgcgcgtcat ggcagaaaat
     4801 cgccgagcga tactgcgtgc caatgtcgtg cccttgccga tttaacgtgg tcggatcatg
     4861 cacggcaaag aacacttcca gcaatgtact gaatgcaatg agattttcgt caaacgtgat
     4921 gcgcacggct tcagcatgtc cgctatggcc ctcgcacacc tcggcataag tcggattggg
     4981 tgtgtaccca ccggtatacc ccgagcgaac gtcatgcacc ccacgcaagc gggtaaatac
     5041 cgcttccaga caccagaaac acccacccgc cagggtggcc gttgccagtg gggtctgttc
     5101 gagtgcagtc atcatcacat cctgtacgta gaaatactta gtgcagctac tgagtacggt
     5161 atatggggtt tagcggaaaa tttccagctt ttttttaaaa aaattccaat taccttgcgc
     5221 cagcatagcg gcggacagtt tcccgcactg tttctgtttg tacagccacc gtgtcggaaa
     5281 ttggctgggc atacccgccc cccatgcata acgccaccgg catgccctgc gcgtcacacg
     5341 catccagcac catggcgtca cgggcagcca gaccagcctg gctaagattc aacttgccca
     5401 gacgatcgct ggctaatgga tccgccccgg ccagatacac cagcaaatcc gggcgggcac
     5461 gggtaaataa tgctggcagg gcactggcca aagcggccag ataaggctcg tcgccggttc
     5521 catcgggaaa ttcgatatcc cagtccccgg gatgtttgcg aaacggaaaa ttcccggcgc
     5581 catgcatgga gaatgtgtaa atactggcat ccccctcaca cagtgctgcc gtgccattgc
     5641 cctggtgcac gtccagatcc accaccagag cacgacggat gcggccttca cgctgcatga
     5701 cccgcaccgc agtcacgatg tcgttgaata cgcaaaaccc actgccgcgc gcgtaagctg
     5761 catgatgggt gcctcccgcc aggttaacgc cgcagcccga ccacagcgcg ctgcgacatg
     5821 ctgcaatggt ggcgcccacc gaacgtcggc tgcgctccac caggccaggc gaccagggaa
     5881 aaccgatttc gcgctgggct gccgccggca acttgccgct gaatacttga gtaatatagt
     5941 cgggatcatg ggccagcacc agttcatcgg catgcgcggc atctggcacg cacaatagct
     6001 cgcccgcaaa tgccgccacg gcatggcgca acatggcgta cttttccatc ggaaaacggt
     6061 gcccggcggg caagggcagc acaaactggt caatatgaaa aatctgcatg atggtggtcg
     6121 gtattttgtc tggctggcga gtacaatccg cttcttttct tgtgcgcatg tccgtcttgc
     6181 gacggcggcg ccagcatgtg tgtcatgaga atcttgcttg ctccaatgca gggactggtt
     6241 gatgccccca tgcgcgccct gctgaccagc atgggcggcg tggatgcctg tgtcaccgag
     6301 tttgtccgcg tcacccacac cttgttgcct gcccgtactt tttaccgcct gtgcccggag
     6361 ctgcatcacg gtggatgcac gccgtccggc gtgccggtca gcgtgcaatt gctgggctca
     6421 gatcctgctt gcctggccga caacgcggcc cgggcggcag agctgggcgc cccggcggta
     6481 gatctgaatt ttggctgccc ggcgcccacc gtcaaccgtc atcgtggcgg cgccgtcttg
     6541 ttgcgcgagc cagagctcct gcatgccatt gtgcgggcag tgcgtgctgc cacgccagcc
     6601 caggtgccgg tgacggccaa gatgcggctg ggctacgatg acaccgccct tgcactggat
     6661 tgtgcccagg ccctggccac agctggcgcc gccgagattg tggtgcacgc ccgcaccaaa
     6721 accgaaggct accgcccgcc cgcccattgg gactggattg cccgtatccg tgaggccgtg
     6781 accgtgaatg tagtggccaa tggtgaaatc tggacacttc aggatgccca gcgctgcctg
     6841 gagatcagtg gcagccagga cctgatgctg ggccgtggtc tggtggcccg ccccgatctg
     6901 gccggacaga ttgccagctg ggcaagcggc cgccccgagg tggtgctgga ctggccgggc
     6961 atgcgcttgg tggtgacaca gttctggggc ctgttgcaag catacgccga tggcgcctac
     7021 ccacaggccc ggctcaagca atggctggcg ctggtggcgc aggcgtttcc ggccgcgcgc
     7081 ccgctgctgg aaagcgtcag gcgtcagcca gacagccata gtgtggccgc ttgtctggcg
     7141 gatgacacct actggcaggt aggcagataa agacagtcaa gtcagggtgc ggattatgac
     7201 tatgctatat tctggcaatg atattacaat ggatacgcat ttgtgctgag gcggtcggcc
     7261 aatgcgttgt aaaggaggac acatgagcac agcgaatcaa ggcattctgc tggtgtgcat
     7321 gggcaatatt tgccgctcac cgaccgccga agcgattttg cgcgcgcaac tgcgcgcccg
     7381 cgggctggaa cacgatgtgc aggtggactc tgccggcacc catgcttatc acacgggcga
     7441 agccccggac gcacgcgcac gccaggcggc acaggctgcg ggctatgccc tggatggcat
     7501 gcgtgcacgt caggtattgg cggatgattt tgcccgcttt gactggattc tggccgctga
     7561 cgcacaaaac ctggccgaac tgcgccgccg ctgtcctgcc cagtggcagg acaaattgcg
     7621 cctgatgcgt gactgggcag ggggcggtga tgtgcctgat ccttactacg gcggcgccca
     7681 ggggtttacc gagatggttg ccatgctgga aaccgcactg gccgccgcac tggatgcctg
     7741 gtatccccct gcaaccggag gcaagccatg acatcacgcg agcttgcaca cgccactgag
     7801 ttgcccgact ggcagcaagc agtcggcgat gtggtatttg actggtgggt ggcggcccgt
     7861 cggctggaat gctccaccca gctgtgcaca ttgcttggcc tgtcgcgcat gcccgcagac
     7921 ccgctggcag agctgaatca gcgtattcac cccgatgacc tgtcggcatt gcaactggca
     7981 ctgaatgcct gctggcattc agcagatggt tggctgcgcc atgaatgccg cgtacgtggc
     8041 ggcgatggcc aggagcgctg gatgctgatc cgcggccaat tgctggagcg tgatggtctg
     8101 ggtgcaccac gacatgtgcg cggggtgctc agcgacatca gcgcccgcaa ggccgacgaa
     8161 gccatgctgc gccgctacga gcatattgtc gccaccagtt cggacgccat gtattttgtc
     8221 gatgccgact gtcgcattgt gctggccaat ccggccatga ccatccgctt tggcctgacc
     8281 ccggaacagg tggtcggccg ccccatggcc acggtgttcg ggctggtggt ctttgatgag
     8341 ctcagccacg acctggcgcg tgcgctggcc ggtgaagcca tgcattttca gcgctggatg
     8401 cattatccgg cgctgggccg gcgttttgtt gatgttcact acgacccttg cggccaggcc
     8461 ggctctcccg cgcatggcgt ggtggtcaca gtgcgcgatc tgaccgattc cgcccgccag
     8521 gcattgctgt tggctcagac ccagcgtgcg gcgcatatcg gcggctggga aatcgactgc
     8581 cagcgccagg aagtgttctg gactgaggaa atgttctgtc tgctgggggt gccggccggc
     8641 ttgcccgcgc aaactttcca gcaatggtca tcacgcatgc cggcagcgca atgggctcag
     8701 ctgtcggcag gcctggctga cgtcaccgct ggtgtacggg ctgaatttgc cctggtggtt
     8761 gaagtcggcg gccaggatgg ccgcacacaa gccctggctt tgcaggcgcg gggtgagcgc
     8821 aatagccggg gtgaagtcat ccgggtattt ggctcgctac aggatgtcac cgcccgccgg
     8881 caggctcagg cacagttgcg tctggctgcc tcagtgtttg aatccagccg cgaagccgtg
     8941 ttcattaccg atgccgagcg acgcatgctg acggtcaatc cggccttttg cacactgacc
     9001 ggcctggacg ctgtggcggt cgtgggccaa tccgtgcgtt gcttaagctc cccacgtcat
     9061 gatgccatgt tttatcgcga catctggcgc acggcggcgc gtgaaggcgg ctggcatggc
     9121 gaagtctggg gcgtgaatgc cgagggtgag gattatccgc aatgggcgtc gctgaccgcc
     9181 gtgcgcgaaa acgatggcgc catcagtcat tacatcggca tgtttgctga tatcagcgcg
     9241 cgcaagagcg ccgaggcacg cgtgcagcaa ctggtcaatt ttgacagtct gaccgacctg
     9301 cccagccgcg cactgctgca acaacgcctg cccgacatgc tggccggcca ccgctatgca
     9361 gactcgattc tgggtgtgct gttcatcgac ctggaccgct tcaagaacat tgccgatacc
     9421 tttggccatc gggccagcga tgaaattctg cgccaggtgg ccacccgcct gcaaggctgc
     9481 atcggccgcg aagatttgct ctgccgccac ggcggcgatg aattcgtgct ggtgctggcc
     9541 aatgtggatc acgctactga actggcgcgc cgcgccagcc tggtgcgcga ggcgctggcg
     9601 cgaccgatgt ggctggatgg caccgagctg gtgctgacgg cctcgatcgg catcagcgtg
     9661 tttccgcaag atggcgacaa tgccgatgag ctgttgcgtc gcgccgatgc cgccttgcat
     9721 cacgccaagg cagaggggcg tgacaatgtg caatttttta ctgaatcact gaatgcgcgt
     9781 gcggcggagt tcctacaatt agaacatagc ctgcgccgag cattggggcg tcatgagttc
     9841 cgtctgcact accagccgca aatcgacatg cgcactggtc aactggtggg catggaggcc
     9901 ttggtgcgct ggcagcaccc ttcgttgggc ctggtcgccc ccagcaaatt catcccggtg
     9961 gcagaagata ccggcatgat tgttgcgatt ggtgagtggg tcatgctgga agcctgccgg
    10021 gaagcccagc gctggcgtga acttgggctg gcggatgtgc cggtagcggt aaatctgtcc
    10081 gccaaccagt tccgtgccgg tgtgcgggat tgtgtggcgc gggccctggc gtacagtggt
    10141 ctgccagcca accggctgga gctggaagtc accgaaagcc tggtgatggg ggctgccgac
    10201 caggtgattg ccagcctgcg cgaactcaaa gaaatggggt tgatcttgtc aattgacgac
    10261 tttggcaccg gctattccag cctgtcttat ctgaaacgct ttccgattga ccggttgaag
    10321 atcgaccgct cttttgtctg cgatatcacc accaatccgg atgacgcggc catcaccacc
    10381 gccatcattc atctgggcca caacctgcgt ctgcatgtgg tggccgaagg agtggaaacc
    10441 cggcagcaat gggatttcct gcgcgaacag ggatgcgatc aggcgcaagg ctactggatc
    10501 agcaagccgc tggacagcga agccctgctg gaatttctgc gctcctggca aggagggttg
    10561 tcgtcatgac cgccagcgcg ccgcgcctgc cagccagctt taccctgacc cccagccata
    10621 cggcagatgt ggtgctgccg ttcacgcctg gcgagcgccg ctttggtagc tcgcccgtgg
    10681 tggtgatcgg cgcatcgact ggcggcaccg aggcgctcaa gacagtgctg tgccagttgc
    10741 cggacaacat gccgccgatt gtggtggtgc agcacatgcc ggaaatgttc actcgttcat
    10801 ttgccaaccg gctcgacagc ctggcccgcc tgcacgtcaa ggaagccgag cagggcgatg
    10861 tactcaagcc cggctgtgcc tatattgccc ctgggcactc tcatgtgctg ctgtccccat
    10921 ccggcaatac gctgacgctg agactgtctg atgcccctga agtcaaccgc catcggccat
    10981 cggtggatgt gctgttccgc tcggccgcca atgtgctggg ccgctcagca attggggtga
    11041 tcctgaccgg tatgggccgc gatggcgcgc gtggcatggc cgaaatgaaa caatgcggcg
    11101 cctataattt ggcccaggac gaagccagtt gtgtggtatt tggcatgccc aaagaagcca
    11161 ttgccttggg tggtgcgcac gaagtattac cgctgaacca gattgcggcg cgcttgacct
    11221 cgctatgtga aaagcgtatt catgagcggc aacattaagt aaacttatgt gaatttctgg
    11281 ctgaaaagcc aactgacgcg gatggagaat tctggcatgc gcaatttggc gcacaacgcg
    11341 cttcctgtgc tggtggtcga ggatgatgcc gacctgcgcg aagcgattgt ggacactctt
    11401 aatctggccg gcttttctac actggaagcc cgtgatggcc agtcggccct ggcctgcctg
    11461 aagcaggagc tggtcgggct ggtgctgtcc gatgcgcaga tgcagccgat ggatggctat
    11521 cagctatttg cagaaatccg ccagcgttac ccaggcattc ccttcattct gatgactgcc
    11581 tatggtgtga ttgagcgcgc cattgaatta ctgcgcaatg gcgcctcgca ctatctgctc
    11641 aaaccgttcg aaccggacgt gttgactgct gaagtggaaa aacacattct caagccgcgc
    11701 tgtaacgata gccatgagct gatagctcac gacccatcga tgcaggacgt gctgcgcctg
    11761 gcatcacggg tcgccgtcac cgacgccacc gtactgctca atggcgaaag tggagtcggc
    11821 aaagaagtgg tggcacgttt tctgcatcgt cactcgatgc gagccgacgc gccatttgtg
    11881 gcggtgaact gtgcagccat tccggaaaac ctgctggaat ctaccttgtt cggtcacgaa
    11941 aaaggggcat tcaccggggc cagcagcgca tttgccggca aatttgaaca agcgcagggt
    12001 ggtacgctgt tactggacga agtttcagaa atgccattga ccctgcaagc caagctgctg
    12061 cgtgtgctgc aggagcgcga gctggagcgg gtaggcggtt cgcgtacggt aaaactggac
    12121 gtacgggtga tttgcaccac caaccgcaac ttggcagaag aagtcaaaaa aggcgcattc
    12181 cgcgaggatt tattcttccg ggtgaatgta tttccgctgg ctattcctgc cttacgtcag
    12241 cgcaagggtg atatcgtgcc gctggcacgt catttcctgg aaaaatatgc cccgacgcac
    12301 aagcggcaat ccgcaccagg cttatccccg gcggctattg aggatttgac ggcctatcgg
    12361 tgggacggta acatccgtga gctggaaaat gtcatacagc gggcgctgat tctgtcgtcc
    12421 ggagacagca tcctgcctca ggatctgatg ctgccatccg atagccagcc tgccgagccg
    12481 gtgattacac cgccgccccg gcaagaccat gcggcccatg ccgcagcgga cgacgacgcc
    12541 agtatgtatg tgctggaaaa acggcacatc ctggatacgc tcgctgccgt caacggggta
    12601 cgcaagctgg ctgccgaaaa actgggcatc agcgaacgta ccctgcgcta caagttgcag
    12661 cgttatcgcg aagaagaaac cgctccggcc ggagcgcaag aacaacatac ggcagaagtt
    12721 ctgccgactc atcaaatgtc aggagaacag catgtctgta caaggcattg atcaggtttt
    12781 gggtgaaatg cgcggcgtct cggccctggc tggtggccgt gcgccggaac ccgtggtcag
    12841 cacgccgccc agcgtggact ttgcggaagt gctgcgctcg tcgattgagc aagtcaacac
    12901 cgttcagcaa acctcacagg atctgcaaaa gcgcttcgag ctgggtgacg aaagcgtgaa
    12961 tctgcacgat gtgatgattt ccttgcaaaa agccagcctg tcgttccaga ccatggtcca
    13021 ggtacgtaac aagctggtga ctgcatacca ggaaatcatg aacacgcaag tgtaagcacc
    13081 ggctgcccgc ccggatgggc aggctgtttc caagtcagtg caagcgcaca gtggcttttc
    13141 cacccactgc gggtggcaac tacatgtaga tttttatggc agagattgca gaacaaggca
    13201 ataacctgaa acagaatcgt gtccgcgaga gcatggagcg tttccgggat cttccgacca
    13261 acaagaagag cctgctctat gttggcattg cggcggtcgc ggcactggtc atcggcaccg
    13321 ctctgtggat gcgtgagccc acctggaaag tcctgttcag caatgtgtct gacaaggacg
    13381 gcgggcaaat caccactgcc ttgcagcaga tgaacgttcc ctacgagttg tcgtcagggg
    13441 gcaccatcgc cgtgcctgca gaaaaggtct acgaaacccg gctcaagctg gctacgcaag
    13501 gtctgcccaa ggccagtggt gtgggttttg agctgatgga caaccagaag ttcggcatca
    13561 gccagttcgc cgaacaggtc aactatcagc gctcaatcga aggcgaactt gcccgcacca
    13621 ttcaggctct gagtgcagtg gagtcggcgc gcgtgcattt ggccattccc aagaacagcg
    13681 tattcttgcg cgagcagcaa aaacccaccg cctcggtggt gctcaacctc aattccggcc
    13741 ggctgatcga ccctacgcag attcatggca tcatgaatct ggtggcgtcc gccgtccccg
    13801 gcttgccgcc gcgcaatatc accatcgtcg accaagaagg caatatgctg tcgacgatgg
    13861 gggaactcaa ccagcaaaac cagaacggcc tggatcagcg tcagctcaat tacgtcaagg
    13921 aaatcgagca gacctatatc aagcgcatcg aaaccattct tgaaccgatt tttggcaagg
    13981 gcaatgcccg cgctgaagtc accgccgaac tggatttctc gcaacgcgag caaacctcgg
    14041 aaaactacac gcccaattct ccacccaatg ctgccgccat tcgcagccag cagatgcacg
    14101 agagcaccga gcgcgagccg ctggggccgg gcggtgtacc gggcgcattg tccaatcagc
    14161 cgcctaatgc tgccgctgca ccgatcacct tgccggctgg tacccaggct ggtgtaagca
    14221 ccacgggcca ggccacacct gacaagagca ccaaaaagga ctctacggtc aactatgaag
    14281 tcgaccgcac cattcagcac gtcaagatgc ctgtcggcat gatcaagcgc gtgtcggctg
    14341 ccgtagtcgt caactacaag aaaaaagtgg acaacaacgg cgaaagcaag tttgtaccgc
    14401 tgacgcagca agagcttggc cagatcaata atctggtgcg tgaggccatg ggttataacc
    14461 cggcccgtgg tgatacgctg aatgtggtca atgcggcttt tgctgataac accgcccgtc
    14521 cggccctgga acagcgcgcc atcgacttta tcaccgaaaa tattttcgac atcgccaagt
    14581 ggggcctgct gctgctggtg ttgctgtatc tgatcttcgg catcatccgc ccgatcgtgc
    14641 gtgaagtgat caacccgccg gtaccggagg aagaatatcc ggacggcccg gccatcgaag
    14701 gtggcgaggg cgaaggcgaa gaaggcgacg aagtcgtgga cgaagtagaa atgcagcgtc
    14761 gcgaatacgc cgccaacctg gaagccgccc gcgagatcat ccgcaacgat ccgcgcatgg
    14821 ctgcccagat tatcaaggaa tggatcagcg atgagtgatg ccggcgtgcg ccgtagcgca
    14881 gtcctgctgt tcagcattgg ccagcaggat gcggtcgagg tgttcaaata cctgggcccg
    14941 aaagaagtac aaaaaatcag taccaacatg actcagatca ccaatctgag cattgacgag
    15001 atcgccacgt cgattcagct gttccgcgac gaggccaaga tgcgtgcgtc gattggtgct
    15061 gcagatgaat acctgcgtca ggtgctggtg gaagcactgg gcgaagacaa ggcctccaat
    15121 ttgctggaca agatttttgc cggcaatgac aactcgggca tcgaaagcct gaagtggatg
    15181 gatccttctt cggctgccga tctgattcgc aacgaacacc cgcagatcat ctctaccatt
    15241 ctggtgcacc tggagccgga tcaatccagc gccatcatgc agcacttctc cgagcgcctg
    15301 cgtaacgacg tgctgctgcg gatcgccaca ctggaaggcg tgcagccgca ggccatccgc
    15361 gaactgaatg acgtgctgac tcagctgttg tccggttccg accgtgtcaa gaagagcgcc
    15421 atcggtggtg tgcagatgac tgccgaaatc ctgaacttca tgggcagttc ctatgaggct
    15481 tcggcgttgg gctcgatccg tgaatatgac ccggagctgg cgcaacgcat tcaggacaag
    15541 atgttcgtgt tcgaaaattt gtgcgatctg gacaaccgtt ccattcaggt catcctgcgc
    15601 gaaatttcgc cggaatcgct cattactgca ttgaagggca ccagccagga gctcaaggac
    15661 aagatcttca agaacatgtc ttcgcgggcg gcagaaatgc tcaaggacga cttcgaggcc
    15721 aaaggcccgg tcaaggtgtc cgaagtggaa gccgaacaga aggaaatcct caagatcgtg
    15781 cgcaagctcg ccgacgatgg tcagatcgtg ctaaccggca agagcgggga agaaggcgtt
    15841 gtcgagtaag atcattccgg ccggtgaatt gcaggggcag gcgctgccca ccctccaatt
    15901 tgcctcgttt ggccagatca cccccaattc tcgcctggcg cgggtagctg cggccgtgac
    15961 ccccaaggtg acgctgcccg ccagtgtcga tctgggggca ctgttggccg ctgccggcgt
    16021 ggacgacccc acggcccagg ctgtcgactc cagttttgct gccgatcccg tcgaggccat
    16081 caatgacagc ctgccaccct caatgcagct ggatgcagat gaggcctctg ccctgctggc
    16141 accagtagaa ggattcgacg aatccggtac gggggaggag attgcccttc aggaagacag
    16201 cgagccggaa cccgaaccgg agcctgcacc acaatggcca accgctgccg aactggaggc
    16261 cattcaccag gaggcctatc aggccggcta tgatgccggt catgcagaag gcttggcgcg
    16321 tggcgccgat gccggtcacc agcaagcatt tgcccacgcc agccagcagt ttgcccaaga
    16381 gcttcaggct tttgccgact tgcaagcctc ctttcaggcc aatctgaccg aactggaagc
    16441 cgaaatggcg ccggccttgc ttaatctggc cgtcaacgcc atgcgcaaac tgctgcatga
    16501 tcatctggcc tgcaagccgg atgccgtgct gggcgtggtt cgccaggcgc tgcacagcat
    16561 tcccggtgaa gtggcccgcg caaccgtgcg cgcccatcca aacgacctgc aagcactcaa
    16621 gaccttcctg cctgccaata ccccggatac tcagtggcaa tttgtggaag atgcctcgct
    16681 gatgtccggt ggctgcctga tcgaattgcc atctgccagt ctggatctga ccctgcaaac
    16741 ccgctggaaa gccctgatgg ccaccttggg agccgatggc gatgtcagcg cttgaacgct
    16801 ggttggctta tgtccaggca tgcggccaca cccttgaaga ggccgcgctc tggcagccca
    16861 agggccggct gacccgcgtc acgggcatgg tcatggaggc tgttggcctg cgtttgccgg
    16921 tgggcagcgc ctgtgtgatt gaaatcgacg aacagcatca tgtggaagcc gaagtcgtcg
    16981 gtttttccgg tgaatgcctg tatctgatgc ctgtgaccaa tgtccatggc ctggtaccgg
    17041 gcacgccggt gtctgcgctg atatcgcatc acccgcccca atacgaacac cacggcgtac
    17101 cgcagaacgc caatggtccg tttggccgtc aggtaccggt aggcgccagc ttgctgggcc
    17161 gtattctgga ctcgctgggc cgaccgctgg atggacaggg cccgattcat cctgacgcgt
    17221 attacccgct gtacgcccaa ccaatcaacc cgctggaccg tactcctgtg cgcgatgtgc
    17281 tggacgttgg cgtcaaggcc atcaatggct tgctgacggt tggccgtggc cagcgtctgg
    17341 gcctgtttgc cggctctggt gtgggtaaga gtgtgctctt gggcatgatg gcccgcttca
    17401 ccaaagcaga tgtggtggtg gtcgggttga ttggcgaacg gggccgagaa gtcaaggatt
    17461 tcatcgaaaa tattctgggc gaagatggtt tggcccgctc ggtggtggtc gctgcccctg
    17521 cggactcgcc gccgctgatg cgtctgcacg gcgcggcata cgcgaccgca ctggccgagt
    17581 atttccgcga ccagggtctg tccgtgctgc tgctgatgga ttcggtcact cgctatgcca
    17641 tggcacagcg ggagattgcc ttggccatcg gcgagcctcc ggtgaccaag ggctatccgc
    17701 catcggtgtt tgccaaactg ccgcagttga tcgaacgcgc cggctgtggt gacaacggcg
    17761 gtgccatcac gggtttttat accgtgctat ctgagggcga tgatcagcaa gacccgattg
    17821 ccgactcggc gcgtgccatt ctggatggtc actttgtgtt gtcccgccat ctggcggaag
    17881 ccggccacta ccccgccatc gacattgaac aatcaatcag ccgggtgatg gtggatgtgg
    17941 tcagcaaaga gcaattgacc accgcacgca acttcaagca actgtattcg cgctatcagc
    18001 gcaaccgtga tctgatcaat gtgggggctt atgtttccgg ctctgaccgc atgctggacg
    18061 aagccatccg tctgcacccc accatggagg ctttcctgat gcaagccatg gacgaatcct
    18121 ttgacctgga cgacacccgg caactgctcg acagtgtgct agtatcgtaa cgatgtgatg
    18181 acatcaaggt ctgctgggct ttgtgccatc catcatctgc atcaccccac aggatgtgtg
    18241 catgcccgtc agcaagtatg aattgttgat ccgcctggcg gcggaaaaat gcgacgatgc
    18301 cgcgcatgcc atgaatgccg ctcgccagcg catggaaacc gccagacagc gcctgcaaca
    18361 actggcccag tttcttgctg attatcttca tcgccgcatc gcccgtggcg aacagggcat
    18421 gagcagtggc cagtgggtgg attaccagca gttcatcgaa cgcctgcagg aagccatcga
    18481 cctgcaacgc cgcgaagtgg acagtagcca gattgcctat gacaaagcca ctgcagcctg
    18541 gcaagaggcg cgcaagaagc tcaaggcact ggaaaaactg caagaacagg aagaactacg
    18601 cgctcgcttg cgcgaagcca agcgagagca gaaacaaacc gacgaatttg ccgcccggat
    18661 tttccgggag tccagaagcc gcatctgacg ctacaccgtg acatggtgcg taaaattgcg
    18721 taaaggtttc tggaatgcaa attgcttgat cacgccaaaa ccttgtccag aggggcgcct
    18781 gcaatgacga taaaagtgac ttcccagtcc acggcagcta atgctgctac ttccctgttc
    18841 cgccagcctg gcgcacaggg tgatacctcc gacctgttcg ccagcttgct ggctggactg
    18901 ggcgatacct tgttgacaga ccccaagacc ggcttgttag gtgatgatgc tgcgctgtcg
    18961 ccactggcgc gcaaactgaa agccgccaaa gccggagatc agccacagca aagtcagtca
    19021 gacctgaatg ccatgctggg cgccgcagcc ttgctgccac aagtccagca agccttggca
    19081 cagcagctga atactcagaa gacagttacc ggtcaggaag gtgaaagccg cgctggcacg
    19141 ggcaccccag gcgtagatcc cgctttgctg gcagccattg ctacccatgg ccgcctgaac
    19201 gcacaggccg gcacagagca ggggaacacc agcgatggca agcctcaggc tcaacccggt
    19261 atgcaagcgc tggccaatgc cttgggccag aagcaaggcg agggcaaacc cgctgaagtg
    19321 caggccagca accagacggc agccccggca gaagttgccg ccaccggcca gaaaacccgt
    19381 ccggaagcgg cagattttac ccagaccttg cgcgaagccg ccgcccaggc cgctgcagat
    19441 actcccgctn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    19501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt tttacccaga
    19561 ccttgcgcga agccgccgcc caggccgctg cagatactcc cgctgttgcc gccaccggcc
    19621 agaaaacccg tccggaagcg gcagatttta cccagacctt gcgcgaagcc gccgcccagg
    19681 ccgctgcaga tactcccgct ccagttgccg cagatggccg tcagattcag gtggcagtgc
    19741 ccagcgctca gcgtggcgcg ccacagcatg tggtcagcat ccccaccccg gttggccagc
    19801 cacaatggac cgatgatgtg ggccagcagg tggtggtgat gatgaacgcc aagctggaaa
    19861 ccgcccagtt gcaagtcacg ccgccagata tgggcccggt ggaaatttca ctgaagatcg
    19921 gtagcgacgg cgctgctcag gtgtcttttg tggcaggcgt agccgaaacc cgtcaggcat
    19981 tggaacaagg catgccgcgc ctcagtgcca tgctggccga taacggcatc cgcctggcag
    20041 atgcccaggt ttcgtccggt caaggtcagg cattccgtga caatacccag tttgcccagc
    20101 aaggtcagca aggtggtggt cagtctggcc acggccagca aagtggccat gttccaggca
    20161 gccaggatca ggctgtactg atgaacttgc ccgatagcgc catcaccagc cagtccgtgt
    20221 ccaacaacga agtcagcatt tacgcatgag gcagattgcg acgggagatc agcggctgtt
    20281 tgcacgctga ttttccgtca tcaggacacc gggcataatg gcgcaatcaa atgccaggac
    20341 atgcgttcgt cacggcattg tgttaatgtt gcgccagaaa aattaatcag cactatccgg
    20401 gaaaatctgg aagaataatt cgctgtctgc acagcaccat caacatcaag gtcgtaacca
    20461 tggcacaaag caaggcaact ccggtggcac ccaaccagaa aacccctggc aacaagcgca
    20521 tgctgatgat tggcgtggcg ctcttggtcg cgctgttgct cctgctcgct ggcgcagtcg
    20581 gctatctgtt cctgaacaaa aacaaggaac acggcgatga gccgacgctg ggggcaccgg
    20641 gcactctgcc ggtcttcgaa aagatggaac cgttcgtgat caacctcagc ggcgagcaag
    20701 gcggcatgct gcaggtagag atggagaccg aactggctgc agaatcttcg cgtgagatcg
    20761 tcaaggctta caatccgaag gtgcgtagcg ccatcatcat gctgctgtcg tcgaaaactt
    20821 cgcaggaact ggccacggct gaaggcaagg ccaagcttcg caatcagatc cgcaagatca
    20881 tcaatgaaac catcggtgcc agcccggatc gcccgctggt ggttggtgtg gtgttcacct
    20941 cgtttattgt tcagcaataa taggggctag gcatggctga cgacattctt tctcaggacg
    21001 aggttgatgc gctactgcgc ggggtctcgg gcacatccga ggccgacgac gcgcatatcg
    21061 cccccgtccg gccctatgac atcggtcgcc aggaacgtat cgttcggggc cggatgccaa
    21121 ccctggaaat catcaacgag cgttttgccc ggaacctgcg gatcggcctg ttcaacttca
    21181 ttcgccgcaa cgccgaaatc tcggtgggtc cggtgcgcgt gcagaagtac agcgaattca
    21241 tccgcaatct ggtggtgccg accaacctga atctggtgca tgtcaaacca ctgcgcggta
    21301 ctggcctgct gattttcgat ccggatctgg tgtttctggt cgtggacaat atgttcggca
    21361 gtgatggccg ctaccatgtc cgtgtggaag gccgtgactt caccccgacc gaacagcgca
    21421 tcatccgccg catgctggac gtggtattta ccgaatacca gaaagcctgg gagccggtat
    21481 accccattga gttcgtgttc atgcgctcgg aaatgaacac ccagtttgcc aatattgcta
    21541 cgccgaccga agtggtggtg gccgtgacat tccgcatcga actgggtgct ggtggcggtg
    21601 atttccacgt gtgcatgccg tattcgatgg tggaaccact gcgcgatttg ctcgcctcca
    21661 ccatggcagc agaccgtacc gaagtggata accgctgggt gaatctgctg tccaagcagg
    21721 ttcaggttgc cgaagtggat ctggtcgcca cactggccga agcccagatc accctggggc
    21781 agatcctcaa cctgaagaag ggtgatgtca tttcggtgga gattcccgaa accatcaacg
    21841 cttccgtctc cggtatcccg gtggcagagt gccattacgg caccatcaac aatcagtatg
    21901 cattgaaggt cgagaaaatc ttggccggga cgcaagaaat gagcgagctg gccattcccc
    21961 gtgatttccg gctcaacaaa cctaatctga gtgggcaata ataatgacga ctgaaaactc
    22021 tgtcagcatg gatgattggg cggctgccat ggccgaacag gcttcggcca ccgaagccgc
    22081 agcgcctgcc agcgcccccg ctccggcagc atcttccacc ccgcagccgg ccatcaatct
    22141 gtttcaggat tttgctggca cgccgatgga catgagtgcc ggaggtgacg cagcagccca
    22201 gaatctggac atgattctgg atattccagt caacatgacc gtggagttgg gtcgcaccaa
    22261 gatcgccatt cgcaatctgc tgcaactggc acagggttcg gtggtggaac tggatggcct
    22321 ggctggtgag ccgatggacg tgctggtcaa cggctgcctg attgcccagg gcgaagtggt
    22381 ggtggtcaac gacaagtttg gtatccgcct gaccgatatc atcaccccgt ccgagcgctt
    22441 gcgccgcttg caaaaataac gcgtgtgatc tccgtgcgtc gcaccctctt ccttgcgctg
    22501 ccctgttggg cagcgcttgc ttttccgggg ctggccttgg ccactaccgt agcacctgcg
    22561 gccaccagcg tcgaactgtc gccggtttct acattgctgc aaaccgtact ggccatggcc
    22621 gtggtgctgg gtgtcattct gggactggct tggctcgcac gccgggtcaa ccccatggcg
    22681 ctcactggca accctgccag cctgcgcgtg gtctccggtg tgatggtggg ccaacgtgag
    22741 cgggtggtgg tggtcgaggt gcaaggtaaa tggctggtgc tcggtgtagc cgcacagtcg
    22801 gtcaacttac tgtgtacact ggacaagcca gaggatgtcc cagctgatgt gccagtggca
    22861 cgcggggcat ttgccgacaa gctgcgcact cttctagaat ctcgcggcat cagcctgcca
    22921 aagaaaccat catgaagttg cactctttta tttccggctt acacacacgg ctgagccgtc
    22981 ctggcttctg gctggtgctg gcgctgtccg tggtaggcat gctggtcagc ggcgtcttgt
    23041 ggatggagcc ggcgatggcc gaaggcctgc cgatgatgaa cagcacgccc gcgcccggcg
    23101 gcggcaacaa ctattcgttg tcgctgcaaa tgctgctgtt tttgacttcg ctcagcttta
    23161 ttcccactgc gctattgatg atgacctcat tcacccgggt ggtgattgtg ttgtcgctgc
    23221 tgcgccaggc gctgggcacc atgcaatcac cgcccaacca ggtgattgtt ggcctgtcgc
    23281 tgttccttac cctgttcatc atgggaccgg tgctggataa ggtatacaaa gaagcctggg
    23341 tgcctttctc ggaagacaag atcaattttt tgcaggcgct ggacaagggc tc
//
LOCUS       DAOPYX010000007        23033 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_2242_length_23033_cov_8.098660, whole genome shotgun sequence.
ACCESSION   DAOPYX010000007 DAOPYX010000000
VERSION     DAOPYX010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 23033)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23033)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23033
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2242_length_23033_cov_8.098660"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..672)
                     /locus_tag="PKN55_00810"
     CDS             complement(<1..672)
                     /locus_tag="PKN55_00810"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNX69813.1"
                     /translation="MTLHAHDLRFAYRARTVLQGVNLSVRPGEVLALLGGNGAGKSTL
                     LRLLLGLLRPSSGEVRLHERPLSAWPRRALARELAYVPQLHQAPFPYRVREVVLLGRL
                     PSHGLLAAPSRADHQAADAALAELELTALAERRYTELSGGERQRVLIARALAQGARLL
                     VFDEPSNGLDYGGQLRLLARMRRLAQAGHGVLFTTHHPDHARQAADRVALLADGRIDA
                     DGAPAE"
     gene            complement(669..1739)
                     /locus_tag="PKN55_00815"
     CDS             complement(669..1739)
                     /locus_tag="PKN55_00815"
                     /inference="COORDINATES: protein motif:HMM:NF013219.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HNX69814.1"
                     /translation="MTACFSNPALPAGLARRWRGLCRVGLPALLVLATPLAMLLALGI
                     GRFPLPPTEVLGYALAKLGWLSLPPARLALLDNLIIDIRLPRVLTAVLVGSALACSGA
                     AYQAVFRNPLVSPGLLGVLAGAAAGAALGMLYGGDWRLVQLGAFALGGAAVLLGVLLA
                     SWCGGALITLVLGGMISGALFTAILSISKYLADPYNQLPAIVYWLMGSLAMANLDDLA
                     RLGPPMLLAMLAMCLCGRGLDALSMGDDEARSLGVPVRALRYGVIALATLASALTVSL
                     AGMIGWVGLLIPHLARLLAGPGNARLLPVSACLGAIFLLCADGLARTLTTTEIPLGIL
                     TELLGIPAFVLVLRRARHGWTA"
     gene            complement(1720..2754)
                     /locus_tag="PKN55_00820"
     CDS             complement(1720..2754)
                     /locus_tag="PKN55_00820"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNX69815.1"
                     /translation="MTRMIDAWRRLSWGALGLALAASAQAAEVVDMVGRRVQVPDHIE
                     RVFGAAPPLNVLLHSLAPDKMIGLSFPIPAEGQRYFPPRLRGLPVVGGIFGMGQQMNP
                     EAVLAMQPDIALAWKSPFIDQARIEAAFAKIGLPVVFIQLDTLADWPAALRYTGRLLG
                     QPARGETLARLVEAPLAKVAQVTARIPLEQRPRVYYAEGPDGLATDCHQSFHTEAIEL
                     AGGYNVYRCQPKDHTGMEKVSLEQVLAFAPDIIIAQDAQFARVVAQDPRWRQVKAVRA
                     GRVYSVPRWPHNWIDRPPSLMRVLGVQWLAGLFYPKQLPFDRLAHTRAFYQQVLGVSL
                     TEQDIDRLFQ"
     gene            complement(2861..5059)
                     /locus_tag="PKN55_00825"
     CDS             complement(2861..5059)
                     /locus_tag="PKN55_00825"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNX69816.1"
                     /translation="MRRKLLPCLLASLCAPSLAAEVTTLPVVEVSASAEDGWLKPERA
                     TPSTVYRVGGEALGAFGNPGGTNPYLNLADLPGVKITPVDAYGLNNMQGGQKGMRVRG
                     EVSTHGVSGTVEGLALNGPGPGPGYLFLFDRENLASLTFAQGALAADQAGLFNSYGAL
                     DSQLLWPRREAGGELSFSMGSERFQRVFARADSGQLASGTAFFISASDTRANKWRGDG
                     QAPGNRDNLEFGLSQALGPLNLKLWYARNDQSQHNYQALTYKQATNLDAYYRSDYPNN
                     RNSAQHYDYNRQDFRNEALLAELDYTFSPTLSLSIKPFYSREQGYYLYAGSSDTQVLK
                     WLIDHTTYGVNSELRGLWGDTEIKLGHSWTSTEPPGPPTTRTQYVIRNGSLQFDRWAM
                     LSKTVDRHEFHSFYLSALHQAGPLTVQGGLRYARETLPSIDAYTVGANSTWDVSADQA
                     YSLASRNAARSVSGRTLAYWLPQLGAGYQLTPQLQARASLGRTIGAPSFDAYNQTPSG
                     AITTSQQYWDQLKPELGVKLDLGLRWQAERAYLEPTLYFSRSRNKGVNVYSEQTRTVY
                     SQNIGQTRGYGLQLSAGLTPLSGLRLFGALSYSRSQFSEDVRTNNGAWLPVQGKQLPD
                     VPRWMANLGAAWQWQGLTVAPSVNYIGSRWGTSTYTERLPGYLTADLNFSYAGKARWG
                     GWELGLSVLNVFDRRYIGQISTSEINTASNGAIYYPGAPRTVVGKVKLTF"
     gene            complement(5292..5492)
                     /locus_tag="PKN55_00830"
     CDS             complement(5292..5492)
                     /locus_tag="PKN55_00830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018151263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HNX69817.1"
                     /translation="MNCKHATWLLSLRQDRPLSFRERLGLRVHLGYCAGCRAYQQQLD
                     FLHRACRQFTPGHTEDPGDSSD"
     gene            complement(5489..6109)
                     /locus_tag="PKN55_00835"
     CDS             complement(5489..6109)
                     /locus_tag="PKN55_00835"
                     /inference="COORDINATES: protein motif:HMM:TIGR02943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNX69818.1"
                     /translation="MPDIADSPSAADAASLLRDASFLASLRRDLLRFACLYLNHDDDS
                     AEDAVQEALLAASRAPERFAGRAAVRTWVFGILKHKLIDEIRRQRRRVYLTPLPSEDE
                     ADDFDALFDARGHWNADDQPPAWSDPDASLEQRQFWQVFETCLTRLPESTARVLMMRE
                     FLDLDTPEICGHFDITPNHCWVILHRARAGLRLCLQTRWFGEEARA"
     gene            complement(6207..7409)
                     /locus_tag="PKN55_00840"
     CDS             complement(6207..7409)
                     /locus_tag="PKN55_00840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012697442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HNX69819.1"
                     /translation="MTHSHSFDPREETPNAARQAAAQRSTWVSVGVNIALSITQMLVG
                     LWAGSQALVADAVHSLSDLVSDFVVLFANRHSAADADKNHPYGHGRFETAASLVIGVL
                     LLSVGVGMLWSAAHKITDPASIGQVHSLALWVAGLALVSKELLFRYMLKIAESVKSSM
                     LVANAWHARSDAASSLVVGVGVAGNLLGFAWLDPLAAALVGFMITHMGWKFSWSALSD
                     LMDHSLDDEEQAAICATLEATPGVRSAHDLRTRRMGDLAMVDVHILVDGRISVSEGHY
                     IAANARQRVLDAHRVLDVMVHIDPEDDSGHSRQMLALPHRAQVEDALAEVLDEHVRAR
                     ISLELHYLNGVLELDAVLDPQACRDAAERERISRQLHALVGQVAGLSQVRIHHTLEAS
                     PSQPAALR"
     gene            complement(7501..7935)
                     /locus_tag="PKN55_00845"
     CDS             complement(7501..7935)
                     /locus_tag="PKN55_00845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX69820.1"
                     /translation="MLHRLLLSLTLAAAPLAAAGNWNVGTQPRNGVVVWIEAPAAPRV
                     NGAGQQAGAMAGAQLGAQHGGAGGAIYGGAAGMMAGEIVERELAAERWRNRAWTVHVQ
                     LDSGPTQTFAYTAPPPWRRGERVRIIGGRLAPMPGARAGAGR"
     gene            complement(7954..9351)
                     /locus_tag="PKN55_00850"
     CDS             complement(7954..9351)
                     /locus_tag="PKN55_00850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008953224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HNX69821.1"
                     /translation="MSRVLCVFALLCPTLAQAAGLNGAELSLLWSLPFAGLLLSIALF
                     PIFAPAFWHHHFGKIALGWALAFLLPCALWLSPADAGNVVVHAMVDEYLPFIVLLFAL
                     YTISGGILITGNLHGSPALNTGILALGAACASLMGTTGAAMLLIRPLLRANDNRRHNV
                     HVVVFFIFLVGNIGGGLTPIGDPPLFLGFLKGVSFSWTFQAMLLPVLTACSLLLLAFY
                     LLDSYYYRKEGVLPRDPTPDNHRLGVQGGFNFLLLAGVVGAVLMSGMWKPGLELNILG
                     VKVGIQNLLRDGMLLGLAGASLAFTSTRIRDGNEFNWDPIAEVGKLFGAIFITMAPAI
                     AILRAGPDGALAQLVGLVSRADGSPIDLMYFWMTGSLSSFLDNAPTYLVFFNLASGDP
                     AQMMTQYSSTLLAISAGAVFMGANSYIGNAPNFMIKAIAEHRGVAMPGFFGYMAWAWV
                     WLFPLCVLLSVLFFL"
     gene            complement(9640..10029)
                     /gene="gloA"
                     /locus_tag="PKN55_00855"
     CDS             complement(9640..10029)
                     /gene="gloA"
                     /locus_tag="PKN55_00855"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017507195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004462 - lactoylglutathione lyase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0051596 - methylglyoxal catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HNX69822.1"
                     /translation="MRLLHTMLRVGNLDASLRFYTEVLGMRLLRRTDYPEGRFTLAFV
                     GYGEEIDHTVIELTHNWDTASYELGNGFGHLAVEVPNAAAACDQVRALGGKVTREAGP
                     MKHGTTVIAFVEDPDGYKIEFIQRGTQ"
     gene            complement(10295..11002)
                     /locus_tag="PKN55_00860"
     CDS             complement(10295..11002)
                     /locus_tag="PKN55_00860"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HNX69823.1"
                     /translation="MAGARLSSHQLATARGPLDYTLARSARKTIGLRVSPAGVEVRAP
                     LRLPHAEIVALLNQRADWLWKHLDRLSALAPAPQLAALQQLWLLGRPLPIVREAGPPG
                     VSLEPERVCVRGARDDAHAAQLLKQRYQAMARRLFAERLQGFAGRWRTPPSALKLSPA
                     RARWGSCTHDGVIRLSWRLLFAPLPVIDYVLAHELAHRIEMNHSSRFWVEVARLCPDW
                     AAQRAWLRQYGQHAPQW"
     gene            complement(11006..11731)
                     /locus_tag="PKN55_00865"
     CDS             complement(11006..11731)
                     /locus_tag="PKN55_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009119840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNX69824.1"
                     /translation="MLVLRNLLYWVGFLLLTPPYSLLAILMLPFPRVLRHRVVTTWTH
                     LMLAWLRLTCGLHYRVRGLENIPNTPSVIACQHQSGWETMALQLIFPRQVWVAKKELM
                     WIPFFGWGLAAVSAIMIDRKSRQAAQQMVDQGRDRMRDGFWIVIFPEGTRMPPGKPGK
                     FKQGAARLAQSLDVPLSPVALNSGEFWPRNSFLKHPGVIDVVIGPPILPAGQSAEALT
                     QALESWTLAQQAALVGRGPCRPD"
     gene            complement(11788..12333)
                     /gene="gmhB"
                     /locus_tag="PKN55_00870"
     CDS             complement(11788..12333)
                     /gene="gmhB"
                     /locus_tag="PKN55_00870"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004285873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HNX69825.1"
                     /translation="MKLVILDRDGVINHDRDDFVKNSMEWVPLPRSLDAIAMLTQAGW
                     HVVVATNQSGLGRGLFDMHALNAMHEKMHHLAHKAGGRIDAVFFCPHKPEAGCACRKP
                     LPGMVLDIAERFGLRPDSLPLVGDSLRDLQAVAAVGGLPILVRTGKGEATLAKGDLPP
                     RTLVFDDLYVAAEYLIRHHAD"
     gene            complement(12420..14495)
                     /gene="glyS"
                     /locus_tag="PKN55_00875"
     CDS             complement(12420..14495)
                     /gene="glyS"
                     /locus_tag="PKN55_00875"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014086141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HNX69826.1"
                     /translation="MKHTLLVELFTEELPPKALPRLAASFAGAIVAGLAEKKLVAADV
                     AAQIFASPRRLGVSIPAVLAVAADEEAVEKIMPVAVALGADGQPSPALRKKLEARGIP
                     LDAVAQFERRLDGKSETFFYRYQAAGAKLADVLAGIVQDALKKLPIPKVMRWGDSDVT
                     FVRPVHKLVMLHGAEVVAGEVLGLTAGRETGGHRFLSAGAVTLTDADSYVSTLQSVGK
                     VVPSFEARRDAIRAGLASRAAALNATLASDDALLDEVTALVEWPVVLEAGFAEDFLKV
                     PQECLILTMQQNQKYFPLLNAEGKLINRFLLASNLETADPSHIIHGNERVLRARLSDA
                     KFFFEQDQKQKLESRVPRLGNVVYHNQIGTQLERVERLESLAGQIAGLIGANVDDARR
                     AARLAKADLVSDMVGEFPELQGIMGMYYARLDGENDTVAAAIEGHYHPRFAGDSLPVG
                     DVAKAVALADKLETVVGIYGIGLIPTGDKDPFGLRRHALGILRMALELPLDITALLAL
                     TAAAFPAGKLSATVAADVFGFMQERLKNLLLADARADEIDAVLALAPTRLDNVRAVLA
                     AVAAFRALPEAATLAAANKRVKNILKKVEGEVSEAVNPALLAEAAEQALAQAITSVRA
                     EVDAAQAAGDLTGALTRLAALKAPVDAFFDGVMVMADDLAVRQNRLALLAQLAGLFNR
                     VADISLLAE"
     gene            complement(14516..14935)
                     /locus_tag="PKN55_00880"
     CDS             complement(14516..14935)
                     /locus_tag="PKN55_00880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX69827.1"
                     /translation="MRRLALLSLSLLAAAPAFAAPDWVVYQRGTLAALAVDRNSVRTE
                     KNNLLRFEHEERYTQQQRDPQLKISFHTRRMLVIGDCLQRRYAFASIDLFDQAGKSVY
                     HSLFQLQDFQWKFQPAPDGSMGAGMLDVVCKLAPAQP"
     gene            complement(14941..15831)
                     /gene="glyQ"
                     /locus_tag="PKN55_00885"
     CDS             complement(14941..15831)
                     /gene="glyQ"
                     /locus_tag="PKN55_00885"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012696231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HNX69828.1"
                     /translation="MLTFQEIILRLQNYWNAQGCALLQPYDMEMGAGTFHTATFLRAL
                     GPEPWRAAYVQPSRRPKDGRYGENPNRLQHYYQYQVVLKPSPPDIQDLYLGSLRELGI
                     DTSVHDIRFVEDDWESPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLDCKPVLGEITY
                     GLERLAMYLQGVENVYDLVWNVLPDGQVVTYGDVFHQNEVEQSTYNFEHSNTELLFRL
                     FNDFEGEAKRLHEVNLALPMYEMVVKCSHTFNLLDARGAISVTERAAYIGRVRALARL
                     VAQAYYDSREALGFPMLKKD"
     gene            complement(15994..17097)
                     /locus_tag="PKN55_00890"
     CDS             complement(15994..17097)
                     /locus_tag="PKN55_00890"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003823045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HNX69829.1"
                     /translation="MTQGFYLGLMSGTSLDGVDAVLADLSAAQPRVLACASLPYADAL
                     RAEILALQPVGDNELDRAARLAQQLARLYAEVCQLALAQAGVSAAQVVAVGCHGQTVR
                     HAPQAGYTVQLADLALLAELTGIDVVGDFRRRDLAAGGQGAPLVPAAHQAWFGDAHEA
                     RVVLNLGGIGNLTRLDPALPVIGFDTGPGNMLMDAWIADCQPGARFDAGGAWAASGRC
                     VPALLADCLADPYFAAPPPKSTGRELFSLDWLHARLAAHPGLAAADVQRTLLQLTVSS
                     AAQALAQHAPATRALYVCGGGALNPLLMAELAAALPHCRVSATDALGVPAMQVEALAF
                     AWLAQRFMQRLPGNLPAVTGAAGGRVLGALYPA"
     gene            complement(17186..18313)
                     /locus_tag="PKN55_00895"
     CDS             complement(17186..18313)
                     /locus_tag="PKN55_00895"
                     /inference="COORDINATES: protein motif:HMM:NF016817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HNX69830.1"
                     /translation="MHRHSHPLPAPSLGVQRDVVSLHFGVPGAGPKVYLQAGLHADEL
                     PGMLVLHHLAQALAAAEQADELIGEVIVVPVANPIGLGQSVLREPVGRFALHSGDNFN
                     RGYPELAEPVRARLAGQWGNDGAHNIALVRAAMRQHLAELRQDAISELDVLRLTLLSL
                     ACDADVVVDVHADTEAIVHLYTETPYWPQAETLARCLGSEVNLLARGSGVACSFDEAC
                     SQPWWQLAESLPAAHPLPLACLAVTLELRGQTDVDEKLAQEDAIRLLAFLRHRGVLRG
                     EPPPLPPLPFPPTPLAGSETVRADRAGLVTFLRPLGDWVDAGEPVCELLDPISGTRST
                     RRASVAGRVYARTTQRYAMLGMELVKIAGSTPFRHGALLTA"
     gene            complement(18444..21365)
                     /locus_tag="PKN55_00900"
     CDS             complement(18444..21365)
                     /locus_tag="PKN55_00900"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNX69831.1"
                     /translation="MTNTDAISTLAQRVRHTLESFFATPTPDQSPMREAIELLCAQVG
                     ADYGAFRADAFCPDYQIQDDRCLSRCPMSESGMPLCQTIPALGHWLLQQPERMCEVFV
                     LSPFFDGQHSFQTAALAVLRVPGQGAALLCVAHRDSAYHWSEAERDALATLVGVFSLL
                     HQRDQAMALATQVEGHERELMAASSDAMLIHDRQRVLFANPACARLVGHESADALLDA
                     PLSSLLHADDLPVLRDQVHAVLSSDTCSEHRWQARLLRRDGPELPVEVSSHRLNFHGR
                     HAVWTTLRDQSAWHDLTRQLHLSETYLDQVLRQLPVILWAVDGDNRFTLCVGTGLRGL
                     PANLTPVVGARVQDCLASLPDFPFQIERARKGERFSALRRFGERLWLETHYTPMPDEQ
                     GRPGVIGMSFDVSDREQAAEQLQLSAQVFQHSGEGIVITDTNGKIIALNRAFERITGY
                     TAQEAVGHTPALLASGRQDGNFYRDMWASLHEQGRWSGEIWNRRKSGEVYPEWLSITA
                     IRNAEGAIHRFIGIFTDMTSRKEKERAIYQLANYDSLTLLPNRHLFNERARLALAAEL
                     AHGGSAAMLFVDLDGFKLVNDITGPHAGDALLVAMARRLHSCLDDDATLARLESDHFL
                     ILAPSVGGGESWRPLVERLLDAVRQPCTVDGAANVHLSASIGVSCFPYDADTVEVLAQ
                     QAESAMQEAKALGGDNFALFKPDLSERAAERFNLFNALKHAHGRDEFRLYVQPQFRLS
                     DGKLIGGEALIRWQHPQWGLVSPARFIPVAEDTHLIRDISDWVLNEACRQRASWPAWT
                     FEGEPLTIAINLSAVHFQDPGLPGKVQEALARYRLDARCLELEVTERTVMGDIKAIIA
                     ILSELKRIGVRLAIDDFGTGYSSLSYLRHFPIDRLKIDRSFVTDIASDTSAAAIVDTI
                     LNLARNLGLSVIAEGVETREQLALLTQRGCDEAQGYLLGYPSPEAEFIKRFLTPPAPA
                     A"
     gene            21865..>23033
                     /locus_tag="PKN55_00905"
     CDS             21865..>23033
                     /locus_tag="PKN55_00905"
                     /inference="COORDINATES: protein motif:HMM:NF017077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE3 domain-containing protein"
                     /protein_id="HNX69832.1"
                     /translation="MKLTLSRKLYGGFGVVLAILAVLVAAALFSTDRLVESKTASQRS
                     YDTVQRALELENQLANISIGIRGFMLTGKEDMLSAVYAGDGEVEKTLKTLRANLADQP
                     EQLARLDKVSQLKGAWMGTAVYPAIEARRGKLNDNTAIGQVSSYYLSLGGTDKGGQIR
                     HELRAFIAAERQALEARNTAVEQSTELARAVLLGGGAIALLLGLLIAAGLGRHIAGRL
                     RHAVDVAHAAAQGDLTVKVRADGHDEVADVLTAFASMQQRLRTLLTDIQHGARNLLDS
                     SGQISAASAHISESSEVQSYSATAMASAVEQLSVSIGHVTESAAEAQRISTQSGELSI
                     SSGDIIAQAVSGIQHIAESVTHAAEEIAQLQAQSSKISAIVTVIQDIADQTNLLAL"
BASE COUNT         4225 a         7542 c         7576 g         3690 t
ORIGIN      
        1 ctcggcgggc gcgccgtcgg cgtctatccg gccgtcggcc agcagcgcca cccggtcggc
       61 ggcctggcgg gcgtggtcgg ggtgatgggt ggtgaacagc acgccatggc cggcctgggc
      121 cagccggcgc atgcgcgcca gcaagcgcaa ctggccgccg tagtccaggc cattgctggg
      181 ttcgtcaaac accagcagcc gcgcgccctg cgccagcgcg cgggcaatca gcacccgctg
      241 gcgctcgccg ccagacagct cggtgtagcg ccgctcggcc agcgcggtca gttccagctc
      301 ggccagcgcg gcgtcggcgg cttggtggtc ggcgcggctg ggggcggcca gcaggccatg
      361 gctgggcaag cgccccagca gcaccacctc gcgcacccga tacggaaacg gcgcctgatg
      421 cagctgcggc acataggcca gctcgcgcgc cagcgcccgt cgcggccagg cggataacgg
      481 gcgttcgtgc aggcgcacct cgccgctgct cgggcgcagc aggcccagca acaggcgcag
      541 cagcgtgctc ttgccagcgc cattgccgcc cagcagcgcg agcacttcgc cagggcgcac
      601 gctcaggttc acgccttgca acacggtgcg ggcgcggtag gcaaagcgca ggtcatgggc
      661 gtgcagggtc atgccgtcca cccatggcgg gcgcggcgca gcaccagcac aaaggccgga
      721 atgcccaaca gttcggtgag gatgcccagc gggatttcgg tggtggtcag cgtgcgcgcc
      781 aggccatcgg cacacagcag aaaaatcgcc cccaggcagg cgctgaccgg cagcagccgg
      841 gcattgccgg gcccggccaa taatcgcgcc agatgtggaa tcaacagccc cacccagccg
      901 atcatgccgg ccagcgacac agtcagcgcc gaggccagcg tggccagggc aatcacccca
      961 tagcgcagcg cgcgcaccgg cacgcccagg ctgcgcgctt cgtcgtcgcc catgctcagc
     1021 gcgtccagcc cgcgtccgca cagacacatg gccagcatgg ccaacagcat cggcgggccc
     1081 agccgcgcca ggtcgtccag attggccatg gccaggctgc ccatcagcca gtaaacaatc
     1141 gccggcagct ggttgtaggg gtcggccagg tatttgctga tggacaggat ggcggtgaac
     1201 aaggcgccgc tgatcatgcc gcccagcacc agggtgatca gcgcgccgcc gcaccagctg
     1261 gccagcagca cgcccagcag caccgccgcg ccgcccaggg caaacgcgcc caactgcacc
     1321 agccgccagt cgccgccata cagcatgccc agcgccgcgc cggccgccgc gcccgccagc
     1381 acgcccagca agccgggcga caccagcgga ttgcgaaaca ccgcctgata cgccgcgccc
     1441 gaacaggcca gcgccgagcc caccagcacg gcggtgagca cgcgcggcag gcggatgtcg
     1501 atgatgagat tgtccagcaa ggccaagcgc gcaggcggca ggctcagcca gcccagcttg
     1561 gccagcgcat agcccagcac ctcagttggc ggcagcggaa aacggccaat gcccagcgcc
     1621 agcagcatgg ccagtggggt ggccagcacc agcagggccg gcaagcccac gcggcagagg
     1681 ccgcgccaac gccgcgccag gccagccggc agggcgggat tactgaaaca ggcggtcaat
     1741 gtcttgctcc gtcaggctca cccccagcac ctgctgataa aacgctcggg tgtgcgccag
     1801 ccggtcaaac ggcagttgct tggggtaaaa cagcccggcc agccactgca cgcccagcac
     1861 gcgcatcagc gacggcgggc ggtcgatcca gttgtgcggc cagcgcggca cgctgtacac
     1921 ccggccggcg cgcacggcct tcacctgccg ccagcgcggg tcttgcgcca ccacgcgggc
     1981 aaactgggcg tcctgggcga tgatgatgtc cggggcaaag gccagcacct gctccaggct
     2041 gactttttcc atgccggtgt ggtccttggg ctggcagcgg tagacgttat aaccgccggc
     2101 cagctcgatg gcctcggtgt gaaacgactg atggcaatcg gtggccaggc cgtccgggcc
     2161 ttcggcgtaa tacacccgtg ggcgctgctc cagcggaatg cgcgcggtga cttgcgccac
     2221 cttggccagc ggcgcctcca ccaggcgggc cagcgtttcg ccgcgcgccg gctggcccag
     2281 cagccggccg gtgtagcgca gcgccgccgg ccagtcggcc agcgtgtcca gctggatgaa
     2341 caccacgggc aggccaatct tggcaaacgc cgcttcaatg cgggcctggt caataaacgg
     2401 gctcttccag gccagggcaa tgtccggctg catggccagc acggcttccg ggttcatttg
     2461 ctggcccatg ccaaaaatgc cgcccaccac cggcagcccg cgcaggcggg gcggaaaata
     2521 gcgctggccc tcggccggaa tcggaaagct caggccaatc atcttgtctg gcgccaggct
     2581 gtgcagcagc acattcagcg gcggcgccgc gccaaacacc cgctcgatat ggtcaggcac
     2641 ctgcacgcgc cggccgacca tatccaccac ttcggcggcc tgggcgctgg cggccagcgc
     2701 cagacccagc gcgccccagc tcagccgccg ccaggcgtca atcattcgtg tcatcagtca
     2761 ttctccggga taacgccgct caccacgccc gcgccacacg tcgagcccgg catgctgttt
     2821 cagccagcac aggggaacag gccggggtgg tgagcgacgc ttagaaggtc agcttgacct
     2881 tgcccaccac cgtgcgcggc gcgccggggt agtagatggc gccattgctg gcggtgttga
     2941 tttcgctggt gctgatctgg ccgatatagc ggcgatcaaa cacattcaac acgctcaggc
     3001 ccagctccca gccgccccag cgcgccttgc cggcatagct gaagttcagg tcggcggtga
     3061 gatagccggg cagccgctcg gtgtaggtgc tggtgcccca gcgcgaaccg atatagttga
     3121 cgctgggggc gacggtcagc ccctgccact gccaggccgc gcccagattg gccatccagc
     3181 gcggcacatc gggcaattgc ttgccctgca ccggcagcca ggcgccgtta ttggtgcgca
     3241 cgtcttcgct gaattgcgac cggctgtagg acagcgcgcc aaacaggcgc agcccgctca
     3301 gcggggtgag gccggccgac agttgcaggc catagccgcg ggtctggccg atgttctgcg
     3361 aatacaccgt gcgggtttgc tcgctgtaga cattcacccc cttgttgcgc gagcggctga
     3421 aatacagcgt cggctccagg taggcgcgtt ctgcttgcca gcgcaggccc agatccagct
     3481 tgacgcccag ctcgggcttg agctggtccc agtactgctg gctggtggtg atggcgccgg
     3541 acggcgtttg gttgtaggcg tcaaacgagg gcgcgccgat ggtgcggccc aggctggcgc
     3601 gcgcttgcag ttgcggggtg agctggtagc ccgcgcccag ttgcggcagc cagtaggcca
     3661 gcgtgcggcc gctgacgctg cgcgcggcat tgcggctggc caggctgtaa gcctggtcgg
     3721 cggagacatc ccaggtgctg ttggcgccga cggtataggc gtcgatcgac ggcagggttt
     3781 cccgcgcgta gcgcaggccg ccctgcacgg tcagcgggcc ggcctggtgc agcgcgctca
     3841 gataaaagct gtggaattca tggcggtcca cggttttcga cagcatggcc cagcggtcaa
     3901 attgcaggct gccattgcgg atcacatact gggtgcgcgt ggtcggcggc cctggcggct
     3961 cggtggaggt ccagctgtgg cccagtttga tttcggtgtc gccccacagc ccgcgcaatt
     4021 cgctgttgac gccataggtg gtgtggtcga tcagccattt cagcacctgg gtgtcgctgc
     4081 tgccggcgta caggtagtag ccttgctcgc ggctgtaaaa cggcttgatg ctcaggctca
     4141 gcgtcgggct gaaggtgtaa tccagctcgg ccagcagcgc ttcgttgcga aaatcctggc
     4201 ggttgtagtc gtagtgctgg gcggagttgc ggttgttggg gtagtcactg cggtaatagg
     4261 catccagatt ggtggcctgc ttataggtaa gcgcttgata gttgtgctgg gattggtcat
     4321 tgcgcgcata ccacagcttg aggttcagcg gccccagcgc ctgactgagg ccaaattcca
     4381 gattgtcgcg gttgcccggc gcctggccat cgccacgcca tttattggcg cgggtatccg
     4441 aggccgagat aaaaaacgcc gtgccgctgg ccagttggcc gctgtcggcg cgggcaaata
     4501 cccgctgaaa gcgttctgag cccatgctga acgacagctc accgcccgcc tcgcggcgcg
     4561 gccataacaa ttggctgtcc agcgcgccat agctattgaa caagccggcc tggtcggccg
     4621 ccagcgcgcc ctgggcaaag gtcaggctgg ccaggttttc ccgatcaaac aaaaacagat
     4681 aacccggccc tggccccggc ccgttcagcg ccaggccttc caccgtgccg gatacgccat
     4741 gggtgctcac ttcgccgcgc acgcgcatgc ctttttggcc gccttgcata ttgttcaggc
     4801 cgtaggcgtc caccggggtg attttcacgc cgggcaggtc ggccagattc aggtaggggt
     4861 tggttccgcc ggggttgcca aacgcgccca gcgcctcgcc gccaacgcgg taaaccgtgc
     4921 tgggcgtggc gcgttctggt ttcagccagc cgtcttcagc ggaggcagag acttccacca
     4981 ccggcagggt ggtcacttcg gcggccaggc tgggcgcgca caggctggcg agcaggcagg
     5041 gcagcagttt gcggcgcatt gggggtgtca tgggccaggg ttcctctcaa gcgtgtcatc
     5101 cggggattgc ccgcagtatt cgtcatgtaa atgtttatat agcgacgaac gggcaacgac
     5161 agctcaagag cacgcaaaag ccaggccaat ggttaatttt gcttaaagca ccttgaaatc
     5221 agcgttttgc tgatagccca acaaagcgaa gggattcgtt atgtcatttt ggctacaacg
     5281 aatgtcagct atcagtcaga agaatcaccc gggtcttctg tgtgcccagg ggtgaactgg
     5341 cggcaggcgc ggtgcagaaa atccagctgc tgctgataag cacggcatcc agcgcaatag
     5401 cccagatgca cgcgcaggcc caggcgttcg cgaaagctga gcgggcggtc ctggcgcagc
     5461 gacagcagcc aggtggcgtg tttgcagttc atgcgcgcgc ctcctcgcca aaccagcggg
     5521 tttgcagaca cagccgcagc ccggcgcggg cgcggtgcag gatgacccag cagtgattgg
     5581 gggtgatgtc gaaatggccg cagatttccg gggtgtccag gtcgagaaac tcgcgcatca
     5641 tgagcacccg tgcggtggat tccggcagcc gggtcaggca ggtttcaaat acctgccaga
     5701 actggcgctg ctccagcgag gcgtccggat cgctccaggc gggcggctgg tcgtcggcgt
     5761 tccagtggcc gcgcgcgtca aagagtgcgt caaaatcgtc agcctcgtcc tcactgggca
     5821 agggggtcag atacacccgc cggcgctggc gacggatttc gtcgatcagc ttgtgcttga
     5881 gaatgccaaa cacccaggtg cgcaccgctg cgcggccagc aaaacgctcg ggcgcgcggc
     5941 tggcggccag caaggcctcc tgcacggcgt cttcggcgct gtcgtcgtcg tggttcaggt
     6001 ataaacaggc aaagcgcagc aggtcgcggc gcaggctggc caaaaagctg gcgtcgcgca
     6061 gcaggctggc ggcgtcggca gcagacggag aatcagcaat atcgggcatg gagcagcact
     6121 gtagcgaatg acaaggccaa aggcaaaact ggcgcggcgc gcacggcgcc gccgcaccag
     6181 aaaaaccaca agcgtatcag aacaatctac cgcagcgccg ccggctggct ggggctggct
     6241 tccagtgtgt ggtgaatgcg cacctggctc agcccggcca cctggccaac cagagcatgc
     6301 agttgccggc tgatccgttc gcgctctgcg gcatcgcggc aggcttgtgg gtcgagcacg
     6361 gcgtccagct ccagcacgcc gttgaggtag tgcagctcca ggctgatgcg ggcgcgcacg
     6421 tgttcgtcca gcacctcggc cagtgcgtct tccacctgcg cccgatgcgg cagcgccagc
     6481 atctgccggc tgtggccgct gtcgtcttcc gggtcgatgt gcaccatcac gtccagcacc
     6541 cggtgcgcgt ccagcacgcg ctggcgggcg ttggcggcga tatagtgccc ttccgagacg
     6601 ctgatgcggc catccaccag aatatgcacg tccaccatgg ccaggtcgcc catgcgccgg
     6661 gtgcgcaggt catgcgcgct gcgcacgccg ggcgtggcct ccagcgtggc gcagatggcg
     6721 gcttgttctt cgtcgtccag gctgtggtcc atcaggtcgg acaatgccga ccagctgaat
     6781 ttccagccca tatgggtgat catgaagccc accagcgccg ccgccagcgg atccagccag
     6841 gcaaagccca gcagattgcc ggccacgcca acgcccacca ccagcgaaga tgccgcgtcc
     6901 gagcgggcgt gccaggcgtt ggccaccaac atgctggatt tcaccgattc ggcgattttc
     6961 agcatatagc gaaacagcaa ttccttgctg accagcgcca gcccggccac ccacagcgcc
     7021 aggctgtgca cctggccaat gctggccgga tcggtgattt tatgcgccgc cgaccacagc
     7081 atgcccacgc ccacgctgag caagagcacg ccaatcacca gcgaggcggc ggtttcaaag
     7141 cggccatggc cgtaagggtg gtttttgtcg gcgtcggcgg cgctgtggcg gttggcgaac
     7201 agtacgacaa agtccgacac cagatccgac agcgaatgca cggcatcggc caccagcgcc
     7261 tgtgagccgg cccacaggcc gaccagcatc tgggtgatcg acagggcgat gttcaccccc
     7321 acgctcaccc aggtgctgcg ttgcgccgcc gcctggcggg cggcgttggg ggtttcttcg
     7381 cgcggatcaa acgaatggga atgcgtcaca atacaaattc catctaaatc aaagacttgg
     7441 cattgtggca caaataaatg aattttcgct tatctgcatg ggcggcgccg acagcagcgc
     7501 ttagcgcccg gcgccagcgc gcgcgccggg cattggcgcc agccgtccgc cgatgatgcg
     7561 cacccgctcg ccgcgtcgcc acggcggcgg cgcggtgtag gcaaaggttt gcgtcgggcc
     7621 gctgtccagc tgcacatgca ccgtccaggc gcggttgcgc cagcgctcgg cggccagttc
     7681 gcgctcgacg atctcgccgg ccatcatgcc ggccgcgccg ccataaatcg ccccgcccgc
     7741 gccgccgtgc tgggcgccca gctgcgcacc ggccatggcg cccgcctgtt ggccggcgcc
     7801 gttcacccgt ggcgcggcgg gcgcttcaat ccacaccacc acgccattgc gcggctgggt
     7861 gccgacattc cagttgccgg ccgccgccag cggcgcggcg gccagggtca aacttagcag
     7921 cagcctgtgc aacatggcgc gctcccggtg ggtttacaaa aagaacagca cgctcaacag
     7981 cacacacagc gggaacagcc acacccaggc ccaggccata tagccaaaaa agcccggcat
     8041 ggccacgccg cgatgctcgg caatggcttt gatcatgaag ttgggcgcat tgccaatata
     8101 gctgttggcg cccatgaaca ctgcgccggc cgaaatcgcc agcagggtgc tgctgtattg
     8161 cgtcatcatc tgcgccgggt cgccgctggc caggttgaaa aacaccagat aggtcggcgc
     8221 gttgtccaga aagctcgaca agctgccggt catccagaaa tacatcaaat caatcgggct
     8281 gccgtcggcg cgcgacacca ggcccaccaa ctgggccagt gcgccatccg ggccggcgcg
     8341 cagaatggcg atggccggcg ccatggtgat gaaaatcgcg ccaaacagct tgcccacttc
     8401 ggcaatcggg tcccagttga attcattgcc atcgcggatg cgggtggaag taaacgccag
     8461 cgacgcgccg gccaggccca gcagcatgcc gtcgcgcagc aggttttgaa tgccgacttt
     8521 cacgccaaga atgttcagct ccagcccggg cttccacatg ccggacatca gcaccgcgcc
     8581 gaccacgccg gccagcagca gaaaattaaa cccgccctgc acgcccagcc ggtggttgtc
     8641 cggggtgggg tcgcgcggca gcacgccttc tttgcggtag taataactgt ccagcaggta
     8701 aaacgccagc agcaacaggc tgcaggcggt gagcaccggc agcagcatgg cctggaaagt
     8761 ccagctgaaa ctcacgccct tgagaaaacc caaaaacagc ggcgggtcgc caatcggggt
     8821 caggccgccg ccgatattgc ccaccaaaaa gataaaaaac accaccacat gcacattgtg
     8881 ccggcggttg tcgttggcgc gcagcagcgg gcgaatcagc agcatggcgg cgccggtggt
     8941 gcccatcagg ctggcgcacg ccgcgcccag cgccagaatg ccggtgttca gcgccggcga
     9001 gccgtgcaga ttgccggtga tcaaaatgcc gccagaaatg gtgtacaggg caaacagcag
     9061 cacgataaac ggcagatatt cgtccaccat ggcgtgcacc accacattgc cggcgtcggc
     9121 tgggctcagc cacagcgcgc agggcagcaa aaaggccagc gcccagccca gggcaatctt
     9181 gccaaagtga tggtgccaga aggccggggc aaaaatcgga aacaaggcaa tcgacagcag
     9241 caggccggca aacggcagcg accacaacaa ggacaactcg gcgccgttca ggccggcggc
     9301 ctgcgccagc gtggggcaca gcagagcaaa cacacacaat acacgggaca tcgcagactc
     9361 ctgtggcgcg cggggggacg cgcggtcagg ccgggcaatt tgcccggcag ggtcggcggc
     9421 aggcagggcc agagggcgcg gcaggggccg caggcaagac aggacaaccg cctggcctga
     9481 gggaatctgg cctgcgccgt cgccaggagg cggcgcaggc gcggtcagcg ggagccgcgc
     9541 cgagctggct tcgcgtcgct caccaaaccc tgtgaagcaa tcctggccag gcgcacgacc
     9601 gcgcgccacg acgccaggcg ggtttggtga gcgggacgct tactgcgtgc cgcgctgaat
     9661 gaattcgatt ttgtagccgt ccgggtcttc gacaaaggca atcaccgtcg tgccgtgctt
     9721 catcggcccg gcttcgcggg tgaccttgcc gcccagcgcg cgcacctgat cacaggccgc
     9781 agcggcattg ggcacttcca ccgccagatg gccaaagcca ttgcccagct cgtagctggc
     9841 ggtgtcccag ttgtgggtga gctcgatcac cgtgtggtcg atttcttcgc cgtaaccgac
     9901 aaaagccagg gtgaagcggc cttccgggta atcggtgcgg cgcagcaggc gcatgcccag
     9961 cacttcggtg taaaaacgca gcgaggcatc cagattgccc acgcgcagca tggtgtgcag
    10021 taaacgcata atcagaaaaa actccaacag gcgccgccat ggcggcgcgg gggtaaaggg
    10081 cgggggctta aagcggctaa gagtcgctca ccaaacccag cgaagcggcc ctggctaggc
    10141 gtgtgcagac agtgcaagcc gtacggcaag gtcacacaac aacgccagga gggttttgtg
    10201 aaccgcgctt aatattcggg cagattcatc accgtttggc gcattgtgcg ccatctgccg
    10261 tcagcgcctt gcgccaggcg gggacatcag gcgctcacca ctgtggcgca tgctgtccgt
    10321 attgcctgag ccaggcgcgt tgtgccgccc aatcgggaca taaacgtgcc acttcgaccc
    10381 aaaagcgcga cgaatggttc atttcgatgc gatgcgccag ttcgtgggcc agcacataat
    10441 caatcaccgg cagcggggca aacagcagcc gccaggacag gcgaatcacc ccatcgtggg
    10501 tgcagctgcc ccagcgcgcg cgcgcgggtg agagcttgag cgcactgggc ggcgtgcgcc
    10561 agcgcccggc aaagccttgc agccgttcgg caaacaggcg gcgggccatg gcctgatagc
    10621 gctgtttcag cagctgcgcg gcgtgggcgt cgtcgcgcgc gccgcgcacg cacacccgct
    10681 ccggctccag gctgacgccg ggcgggccgg cttcgcgcac aatcggcagc ggccggccca
    10741 gcagccacag ttgttgcagc gccgccagct gcggcgcggg ggccagcgcg ctcaggcggt
    10801 caaggtgttt ccacaaccag tcggcgcgct ggttcagcag ggcgacaatc tcggcgtgcg
    10861 gcaggcgcag cggcgcgcgc acttccaccc cagccgggct gacgcgcaag ccgatggtct
    10921 tgcgcgcgct gcgcgccagc gtgtagtcca gcggcccgcg cgcggtggcc agctggtgcg
    10981 agctaaggcg cgcgccggcc atgtttcagt ccgggcggca cggcccacgg ccaaccagcg
    11041 ccgcttgttg cgccagcgtc cagctttcca gcgcctgggt cagcgcttcg gcgctttggc
    11101 cagcgggcag gatgggcggg ccaatcacca cgtcgatcac gccggggtgc ttcagaaaag
    11161 aattgcgcgg ccagaattca cccgaattca gcgccaccgg cgacaagggc acatccagcg
    11221 actgcgccag ccgcgccgcg ccctgcttga acttgcccgg cttgcccggc ggcatgcgcg
    11281 tgccttccgg gaagatcaca atccagaagc cgtcgcgcat gcggtcgcgg ccctggtcca
    11341 ccatctgctg ggcggcctgg cggcttttgc ggtcgatcat gatggcgctg accgccgcca
    11401 gcccccagcc aaagaacgga atccacatca gttctttttt ggccacccac acctgacgcg
    11461 gaaaaatcag ttgcagggcc atggtttccc agccggactg gtgctggcag gcaatcaccg
    11521 agggggtgtt ggggatgttt tccagcccgc gcacgcggta atgcaggccg caggtcagcc
    11581 gcagccaggc cagcatcagg tgcgtccagg tggtgaccac gcgatggcgc agcacgcgcg
    11641 gaaacggcag catcaggatg gccagcagcg aatacggcgg ggtcagcagc aaaaagccga
    11701 cccaatacag caggttacgc aacactaaca tgagcaggaa caatcgcttt ctacaaacac
    11761 ggacaaaaac cggcgcgcgg cgcgcgctca gtcggcatgg tgacggatca gatattccgc
    11821 cgccacatac aaatcgtcaa acaccagggt gcgcggcggc aaatcgccct tggccagggt
    11881 ggcctcgccc ttgccggtgc gcaccagaat gggcaggccg cccaccgccg ccaccgcttg
    11941 caggtcgcgc aggctgtcgc ccaccagcgg caggctgtcc gggcgcaggc caaagcgctc
    12001 ggcgatgtcc agcaccatgc ccggcagcgg cttgcggcag gcacagccag cttcgggctt
    12061 gtgcgggcag aaaaacaccg cgtcgatgcg cccgccggcc ttatgcgcca gatggtgcat
    12121 tttttcgtgc atggcgttca gcgcgtgcat atcgaacaag ccccggccca ggccggattg
    12181 attggtggcc accaccacat gccagccggc ctgcgtcagc atggcgatag cgtccaggct
    12241 gcgcggcagc ggcacccatt ccatgctgtt tttgacgaaa tcatcgcgat cgtggttgat
    12301 gacgccgtcg cggtcgagga tgacgagttt catgcattcc atccagaaaa aaccgcccgg
    12361 acagcacacg gcccgccgtt gccagcgggc cgggcgcaag ggcgcaaaaa gagcgtgggt
    12421 tattccgcca gcaaggaaat atccgccacc cggttgaaca agcccgccaa ctgcgccagc
    12481 agggccaggc ggttctggcg cacggccagg tcgtcggcca tcaccatcac gccgtcaaag
    12541 aaggcgtcca ccggggcttt cagcgccgcc aggcgggtca gcgcgccggt caggtcgcca
    12601 gcggcctggg cggcgtccac ctcggcgcgc acgctggtga tggcctgggc cagcgcctgt
    12661 tcggcggctt cggccagcag ggccgggttg accgcctcgc tgacttcgcc ttcgaccttt
    12721 ttcaggatgt ttttcacccg cttgttggcg gcggccaggg tggcggcttc tggcagggcg
    12781 cggaaggcgg ccacggcggc cagcacggcg cgcacattgt ccaggcgggt gggcgccagc
    12841 gccagcacgg cgtcgatttc atcggcgcgg gcgtcggcca gcagcaggtt tttcagccgt
    12901 tcctgcataa agccaaacac atcggcggcc acggtggcgg acagcttgcc ggccgggaag
    12961 gcggcggcgg tcagcgccag cagcgcggtg atatccagcg gcagctccag cgccatgcgc
    13021 agaatgccca gcgcgtggcg gcgcaggcca aacgggtctt tgtcgccggt cgggatcagg
    13081 ccgatgccat aaatgccgac cacggtttcc agcttgtcgg ccagcgccac ggccttggcc
    13141 acgtcgccca ccggcaggct gtcgccggca aagcgcgggt ggtagtggcc ttcgatggcg
    13201 gcggcgacgg tgtcgttttc gccatccagg cgggcgtagt acatgcccat gatgccttgc
    13261 agctcgggga actcgccgac catatcgctg accaggtcag ccttggccag gcgggcggcg
    13321 cggcgggcgt cgtccacatt ggcgccaatc agcccggcaa tctggccggc caggctttcc
    13381 agccgctcga cgcgctccag ctgggtgcca atctggttgt ggtacaccac attgcccaga
    13441 cgcggcacgc ggctttccag tttttgcttt tggtcttgct caaagaaaaa cttggcgtcg
    13501 gacaagcggg cgcgcagcac gcgctcgttg ccgtggatga tgtgcgaggg gtcggcggtt
    13561 tccaggttgg acgccagcag gaagcggttg atcagcttgc cttcggcgtt cagcagcggg
    13621 aagtacttct ggttttgctg catggtcagg atcaggcatt cctgcggcac tttcaggaag
    13681 tcttcggcaa agccggcttc cagcaccacc ggccattcca ccagcgcggt cacttcgtcc
    13741 aacagggcgt cgtcgctggc cagggtggcg ttcagcgcgg cggcgcggct ggccaggccg
    13801 gcgcgaatgg cgtcgcggcg ggcttcaaaa ctggggacca ccttacccac gctttgcagc
    13861 gtgctgacat agctgtcggc gtcggtcagc gtgactgcgc cagccgacaa aaagcggtgg
    13921 ccgccggttt cgcggccggc cgtcaggccc agcacttcgc cagccaccac ctcggcgccg
    13981 tgcagcatca ccagcttgtg caccggacgg acaaaggtca cgtcagaatc gccccagcgc
    14041 atcaccttgg ggatgggcag ctttttcagc gcgtcctgca cgatgccggc cagcacgtcg
    14101 gccagcttgg cgccagcggc ctggtagcgg tagaaaaagg tttcgctctt gccgtccagc
    14161 cgacgctcga actgggcgac ggcgtctagc ggaatgccgc gcgcttccag ttttttgcgc
    14221 agcgccgggc tgggctggcc atcagcgccc agcgccaccg ccaccggcat gattttttca
    14281 acggcttctt cgtcggcggc caccgccagc acggcgggaa tgctcacgcc caggcggcgc
    14341 gggctggcaa aaatctgcgc ggcgacgtcg gcggcgacga gttttttctc ggccaggccg
    14401 gccacaatcg cgccggcaaa gctggcggcc aggcgcggca gcgccttggg tgggagttct
    14461 tcggtaaaca gttcgacaag cagggtgtgt ttcatggcgt caggtcaggc attcgttagg
    14521 gttgcgcggg cgcgagcttg cagaccacat ccagcatgcc cgcgcccatc gagccgtccg
    14581 gcgcaggctg gaatttccat tggaaatcct gcaactggaa cagcgagtgg tagaccgact
    14641 tgccggcctg gtcaaagaga tcaatcgagg caaaggcgta gcggcgctga aggcagtcgc
    14701 cgatcaccag catgcgtcgg gtgtggaagg aaattttcag ctgcgggtcg cgttgctgct
    14761 gggtgtagcg ctcttcgtgc tcaaagcgca gcagattgtt tttttccgtc cgcaccgaat
    14821 tgcggtccac cgccagcgcg gccagcgtgc cgcgctggta caccacccag tccggggcgg
    14881 cgaaggcggg cgcggcggcc agcagggaca atgacaacag ggcaagtcgg cgcatcagcg
    14941 tcagtccttt ttcagcatcg ggaagcccag cgcttcgcgc gaatcgtagt aggcctgcgc
    15001 caccagccgc gccagcgcgc gcacccggcc aatgtaggcg gcgcgctcag tcaccgaaat
    15061 cgcgccacgg gcgtcgagca ggttgaaggt gtgcgagcac ttcaccacca tttcatacat
    15121 cggcagcgcc agattcactt cgtgcaggcg cttggcctcg ccttcaaagt cgttgaacag
    15181 gcggaacagc agctcggtgt tgctgtgctc gaagttatag gtggattgct cgacttcgtt
    15241 ctggtggaac acatcgccat aggtgacaac ctggccatcc ggcagcacgt tccacaccag
    15301 gtcgtagacg ttttccacgc cttgcagata catggccagg cgctccaggc cataggtgat
    15361 ctcgcccagc accggcttgc aatccaggcc gcccacttgc tggaagtagg tgaactgggt
    15421 gacttccatg ccgttcagcc agacttccca gcccaggccc caggcgccca gcgtcgggct
    15481 ttcccagtcg tcctcgacaa agcggatgtc gtgcaccgag gtgtcgatgc ccagctcgcg
    15541 caggctgccc aggtagaggt cttgaatatc cggcgggctg ggtttcagca ccacttgata
    15601 ctggtaatag tgttgcaggc ggttggggtt ttcaccgtag cggccatcct tggggcggcg
    15661 cgaaggctgc acataggcgg cgcgccacgg ctccgggccc agcgcccgca ggaaggtggc
    15721 ggtatggaag gtgcctgcgc ccatttccat gtcgtaaggt tgcagcaacg cgcagccttg
    15781 cgcgttccag tagttctgca agcggaggat gatttcctga aaggtgagca tagtcccggc
    15841 acggcaacgt gaaaaataac acgtcatttt actggcgttt gcgggtgggg gaaaggtggc
    15901 ggtgattctg ctgctgccag cccagcccga tggcgggatc gccagctgcc gtgatagtgt
    15961 ggcccagccg ggaaaacccc tcattggcgg cgatcaggcc ggatacagcg cccccagcac
    16021 ccgcccgcct gccgcgccgg tgacagcggg cagattgccc ggcaagcgct gcataaaccg
    16081 ctgcgccagc caggcaaagg ccagtgcttc cacttgcatg gccggcacgc ccagtgcgtc
    16141 ggtagcgctc acccggcaat gcggcagcgc ggcggccagc tcggccatca gcagcgggtt
    16201 cagcgcgcca ccaccgcaca catacagcgc acgggtggcg ggcgcgtgct gggcaagcgc
    16261 ctgtgccgcg ctgctgacgg tcagctgcaa caaggtgcgc tgcacatcgg ccgctgccag
    16321 accagggtga gcggccagcc gcgcgtgcag ccagtccagc gagaacagct cgcggccggt
    16381 gctcttgggc ggcggtgcgg caaaatacgg gtcggccaga caatccgcca gcagcgcagg
    16441 cacgcaacgc ccgctggccg cccaggcgcc gcccgcatca aaacgcgccc ccggctggca
    16501 atcggcaatc caggcgtcca tcagcatatt gcccggcccg gtgtcaaagc cgatgacggg
    16561 caaggccgga tccagccggg tgaggttgcc gatgccgccc aggttcaaca ccacccgcgc
    16621 ttcgtgcgcg tcgccaaacc aggcctgatg cgctgccggc accagcggcg cgccctggcc
    16681 gcccgccgcc agatcgcgac gacgaaaatc gcccaccaca tcaatgccgg tcagttccgc
    16741 cagcaaggcc aaatcggcca gttgcacagt gtagccagcc tgcggcgcgt ggcgcacggt
    16801 ctggccatga caccccaccg ccaccacctg cgccgcgctg acgccagcct gcgccaaggc
    16861 cagctggcac acttcggcgt acaagcgcgc cagttgctga gccaagcggg cggcgcgatc
    16921 cagttcatta tcgcccaccg gctgcaaggc cagaatctcg gcgcgcagcg catccgcata
    16981 cggcaggctg gcgcaggcca gtacgcgcgg ttgggcggcg gataaatccg ccagtacggc
    17041 gtctacgccg tccaggctgg tgccggacat caggccgaga taaaaacctt gggtcatatg
    17101 tggtgaggtc tgcatcagtc agtcaggaaa aagggcgatc gtgtggcatc gccgtgcggg
    17161 ggcatgagat gtttcgggca ggtatttacg ccgtcaacaa ggccccgtgg cgaaacggcg
    17221 tgctgccggc gattttgacc agttccatgc ccagcatggc gtagcgctgt gtggtgcggg
    17281 cgtacacgcg gccggccacg ctggcgcggc gggtgctgcg cgtgccgctg atcgggtcga
    17341 gcagttcaca aaccggctca ccagcgtcca cccaatcacc cagggggcgc agaaacgtga
    17401 cgaggccggc ccggtctgcg cgcacggttt ccgacccggc cagaggggtg ggcggaaacg
    17461 gcaacggcgg caggggcggg ggctcgccgc gcaacacgcc acggtggcgc aaaaaggcca
    17521 gcaatcggat ggcgtcttcc tgggccagtt tttcatcaac gtcggtctgg ccgcgcaatt
    17581 ccagcgtaac ggccaggcag gccagcggca gcggatgggc agccggcagg ctttcggcca
    17641 gttgccacca aggctggctg catgcttcgt caaacgagca ggccacgccg ctgccgcgcg
    17701 ccagcaggtt gacttcactg cccaggcagc gcgccagggt ttccgcctgc ggccaatacg
    17761 gggtttcggt atacagatgg acaatcgcct cggtatcggc gtgcacgtcc accaccacat
    17821 cggcatcgca ggccaggctc agcagggtga gccgcagcac gtccagctcg ctgatagcgt
    17881 cctggcgcag ctcggccagg tgctggcgca tggcggcgcg caccagggcg atattgtggg
    17941 cgccgtcatt gccccattgc ccggccagcc gggcgcgcac tggctcggcc agctcggggt
    18001 agcctcgatt gaaattgtca ccactgtgca gggcaaaacg gcccaccggc tcacgcagca
    18061 cgctttgccc caggccgatc gggttggcca ccggcacgac aatcacttct ccaatcagct
    18121 catccgcttg ctcggccgcc gccagcgctt gcgccagatg gtgcagcacc agcatgcccg
    18181 gcagttcgtc ggcgtgcagg ccggcttgca gatacacctt gggcccggcg ccgggcacgc
    18241 caaaatgcag gctgaccacg tcgcgctgca cacccaggct gggcgcgggc aacgggtggg
    18301 aatgacggtg catgcaagct ctccgtagga cgggcgggga cagcatatgg tatgtcaact
    18361 tgctgcgccg ctccagcggc ttggcggaca gctcaggggg caacggtcat cctgtggcct
    18421 gacatgcggc aggatgaccg tattcaggcg gcaggcgcgg gcggtgtcag aaagcgtttg
    18481 atgaattcgg cttccgggct ggggtagccc agcagatagc cctgggcttc gtcgcagccg
    18541 cgctgggtca gcagggccag ctgttcgcgg gtttccacgc cctcggcaat caccgacagg
    18601 cccagattgc gcgccagatt cagaatggtg tccacgatgg cggcggcgct ggtgtcgctg
    18661 gcgatgtcgg tgacaaacga gcggtcgatt ttcagccggt caatcggaaa gtgccgcaga
    18721 taagacaagg acgaatagcc ggtgccaaag tcgtcaatgg ccaggcgtac gccaatgcgc
    18781 ttgagctcgg acagaatggc gatgatggcc ttgatgtcac ccatcaccgt gcgctcggtg
    18841 acttccagct ccagacagcg ggcgtccagc cggtaacggg ccagtgcctc ctgcactttg
    18901 ccgggcaaac ccggatcctg aaaatgcacg gcggaaagat tgatggcgat ggtcagcggt
    18961 tcgccctcga aggtccacgc cggccagctg gcgcgctggc ggcaggcttc gttgagcacc
    19021 cagtcgctga tgtcgcggat cagatgcgtg tcttccgcca ccgggataaa acgcgccggg
    19081 ctgaccaggc cccattgcgg gtgctgccag cggatcagcg cttcgccgcc aatcagcttg
    19141 ccgtcgctca gacggaattg cggctgcaca tacaggcgga attcatcacg accatgggcg
    19201 tgcttgaggg cgttgaacag gttaaagcgt tcggcggcgc gctcggacaa atccggcttg
    19261 aacagggcaa aattatcgcc gcccagcgcc ttggcctctt gcatggccga ttcagcctgc
    19321 tgggccagca cttccaccgt atcggcgtca tacggaaagc agctgacgcc aatgctggcg
    19381 ctcaggtgca cattggcggc gccgtccacc gtgcatggct ggcgcacggc atccagcaga
    19441 cgttcgacca gcgggcgcca cgactcgccg ccccccacgc tgggggccag aatcagaaaa
    19501 tggtcgcttt ccagccgggc cagcgtggcg tcgtcgtcca gacagctgtg cagccgccgc
    19561 gccatcgcca ccaggagcgc atcaccggca tgtggcccgg tgatgtcgtt gaccagcttg
    19621 aagccatcca gatcgacaaa cagcatggcc gccgagccgc cgtgcgccag ttcggcagcc
    19681 agcgccagcc gggcgcgctc gttgaacagg tggcgattgg gcagcagcgt cagactgtca
    19741 taattggcca gttgatagat ggcgcgttct ttttccttgc ggctggtcat gtcggtgaaa
    19801 atgccgataa agcgatgaat cgccccctcg gcattgcgaa tggcggtgat cgacagccat
    19861 tccgggtata cctcgccgga tttgcgtcgg ttccagattt caccggacca gcgcccctgc
    19921 tcgtgcagcg acgcccacat atcgcggtaa aagttgccgt cctggcggcc agaagccaat
    19981 agcgccgggg tatggcccac ggcttcttgt gcggtgtagc cggtgatgcg ctcaaacgcc
    20041 cggttcagtg cgatgatctt gccgttggtg tcggtgatga caatcccttc accggagtgc
    20101 tggaacacct gcgccgacag ctgcaattgc tcggcggcct gctcacggtc gctgacgtca
    20161 aacgacatgc cgatcacgcc ggggcgacct tgctcgtccg gcattggggt gtagtgggtt
    20221 tccagccaca ggcgctcgcc aaagcggcgc agggcgctga agcgctcgcc cttgcgcgcg
    20281 cgctcaattt gaaacgggaa atccggcagg ctggccaggc aatcctgcac ccgcgcgccc
    20341 accaccgggg tgaggttggc cggcaggccg cgcaggccgg tgcctacgca cagggtaaag
    20401 cggttgtcgc cgtccactgc ccacagaatc actggcagct ggcgcagcac ctgatccaga
    20461 taggtttcgc tcaggtgcag ctggcgggtg aggtcgtgcc aggcggattg gtcacgcaag
    20521 gtggtccaca ccgcgtgacg accatgaaaa ttcagccggt gcgagctgac ttccactggc
    20581 agctccgggc cgtcgcggcg cagcagtcgt gcctgccagc gatgctcgct acaggtgtcg
    20641 ctggacaata ccgcatgcac ctgatcgcgc agcacgggca gatcgtcagc gtgcagcagg
    20701 gaggacagtg gcgcatccag cagggcatca gccgattcgt ggcccaccag ccgggcgcaa
    20761 gccgggttgg cgaacaacac gcgctggcgg tcgtgaatca gcatggcgtc gctgctggcg
    20821 gccatcagct cgcgttcgtg gccttctacc tgggtggcca gcgccatggc ctggtcgcgc
    20881 tggtgcaaca ggctgaacac gcccaccagg gtggccagcg catcgcgctc ggcctcgctc
    20941 cagtgatatg cactgtcacg gtgcgccacg cacagcaaag ctgccccctg accgggcacg
    21001 cgcagcaccg ccagcgccgc agtctggaag ctgtgctgac catcaaaaaa cggcgacagc
    21061 acgaatacct cgcacatgcg ttccggctgt tgcagcaacc agtggcccag ggccgggatg
    21121 gtctggcaga gcggcatgcc cgactcactc atcgggcagc ggctcaggca gcgatcatcc
    21181 tggatctggt agtccgggca aaaggcatcc gcgcggaagg cgccgtaatc ggcgcccacc
    21241 tgggcacaca gcagttcaat cgcctcacgc atcggcgact ggtctggcgt gggcgtggca
    21301 aagaaacttt ccagggtgtg ccggacccgc tgcgccaatg tcgaaatggc gtcagtgttg
    21361 gtcatgtccg cgacggaact ggctgatgga tgagacagac tggcatctcc cttgctaaat
    21421 ctacggggct gaatcggcca tgcggtgctg ccatagggtg gcaggcaggg caaggcggga
    21481 gctcgttcag gcggcgcgca gtgtaacacg cctgcaacgg gcaaccagcc gggaatccgc
    21541 gcattgtctg acttggcgca aattcccgac ctatggtttg gccagggctt tgtccgtatg
    21601 ctacggcccg gattgcattc gggccgcacg gacgtgcacc acacgatcca taaccagctt
    21661 tgcaaaatgg ttacacaaaa gtcatgtttt gaccatgcac catttgagct tagcaaaaaa
    21721 accggcagcg ttcaccccgc atagccggtg ctcaggcgtc cgcctacccc atggcgggca
    21781 taaagaagat gtgtgccagc gcttcctacc cggaaggccg ccggcccgcg ccacagccgg
    21841 cgcggatcac gagagagacg attcatgaag ctgaccctgt cgcgcaagct gtacggggga
    21901 tttggcgtgg tgctggccat tctggccgtg ctggttgccg ctgccttatt ctctaccgac
    21961 cgtctggtcg aatccaaaac cgccagccaa cgcagttacg acaccgtgca gcgtgcgctt
    22021 gagctggaaa accagctggc caatatcagc attggcattc gcggctttat gctcaccggc
    22081 aaagaagaca tgctcagcgc cgtgtatgcc ggcgatggcg aagtggaaaa aaccctgaaa
    22141 accctgcgcg ccaatctggc cgatcagcca gaacaactgg cgcggctgga taaagtctcc
    22201 cagctcaagg gcgcgtggat gggcaccgct gtgtatccgg ccattgaagc gcggcgcggc
    22261 aagctcaacg acaacaccgc cattggccag gtgtccagct attatctgtc gctgggcggc
    22321 actgacaagg gtgggcagat tcgccatgag ctgcgcgcct ttattgccgc tgagcgccag
    22381 gcgctggagg cgcgcaacac ggccgtcgag cagtccactg aactggcgcg cgccgtgctg
    22441 ctgggcggcg gcgccatcgc cctgctgctg ggtttattga ttgccgccgg cctgggccgc
    22501 catattgccg gccgcctgcg ccatgcggtg gacgtggcgc acgccgccgc ccagggcgac
    22561 ctcaccgtca aggtgcgcgc cgatggccac gacgaagtgg ctgacgtgct gaccgccttc
    22621 gccagcatgc aacagcgcct gcgcaccctg ctgaccgaca tccagcacgg cgcgcgcaat
    22681 ctgctggatt cgtccgggca gatttccgcc gcctcggcgc acatcagcga atccagcgaa
    22741 gtgcaatcct actccgccac cgccatggcc tcggcggtgg aacaactgtc ggtgagcatt
    22801 ggccatgtca ccgaaagcgc cgccgaagcg cagcgcatct ccacccaatc cggcgagctg
    22861 tcgatcagca gcggtgacat catcgcccag gcggtcagcg gcattcagca tattgccgaa
    22921 agcgtgaccc acgccgccga agaaatcgcc cagctgcaag cccagtccag caagatttcg
    22981 gcgattgtca ccgtgattca ggacatcgcc gaccaaacca atctgctggc gct
//
LOCUS       DAOPYX010000008        21717 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_2453_length_21717_cov_10.025482, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000008 DAOPYX010000000
VERSION     DAOPYX010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 21717)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21717)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21717
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2453_length_21717_cov_10.025482"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            64..1353
                     /gene="gltA"
                     /locus_tag="PKN55_00910"
     CDS             64..1353
                     /gene="gltA"
                     /locus_tag="PKN55_00910"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012697885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004108 - citrate (Si)-synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HNX69833.1"
                     /translation="METNRKVTLSYDEGKTLDMPVMSGTLGPDVVDIRTFAKTGMFTF
                     DPGFLATAACESSITFIDGDLGQLYYRGYPIEQLAEHSDFLESCYLLLNGELPTAAQF
                     AEFNRKIMRHNMLNDQIMSFFKGFRRDAHPMAVMVGVVGALSAFYHDSLDINDAHHRE
                     VSAHRLIAKVPNIAAQAYRYNKGLPFSYPKNGLTYSENFLHMMFSTPCEEYKVNKVLA
                     KALDRIFILHADHEQNASTSTVRLAGSSGANPFACIAAGIACLWGPSHGGANEAVLKM
                     LDEIGSVENVPAFMEGVKAKTHKLMGFGHRVYKNFDPRAKIMKETCDEVLNELGLRDD
                     PKFQLAMKLEEIALSDPYFIERKLYPNVDFYSGIVLSAIGIPVSMFTPIFALARTVGW
                     ISHWTEMISDPGMKIGRPRQLYTGSVRRDYAQVADRK"
     gene            1624..4443
                     /locus_tag="PKN55_00915"
     CDS             1624..4443
                     /locus_tag="PKN55_00915"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018747792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HNX69834.1"
                     /translation="MMQTLQSHSYLFGGNAPFIEELYEQYLADANAVPGEWREYFDKL
                     QQQGGVARDVPHQPIQESFIQLAKQPRIGAAPSASADGASLQKQVAVLKLISAYRVLG
                     SRWANLDPLKRMDQSPVRELDPSTHGLSDADMAVQFNTGSLVGQQKKLPLSEILKNLK
                     QTYCGNIGLEYMHITNSEQKHWIMQRFEADLSTPRFGKDAKKRILKQVTAAETLERYL
                     HTKYVGQKRFSLEGGESMIAALDNLIQNSSSIGVQEIIIGMAHRGRLNVLVNTLGKLP
                     RDLFAEFEGRPAVELPSGDVKYHMGFSSDIPTANGPVHVSLAFNPSHLEIVNPVVEGS
                     VRARQQRRGDHERRQVVPVLIHGDAAFGGLGVNQGTFNLSGTRGYGTGGTLHLVVNNQ
                     VGFTTSDTRDMRSTLYCTDVAKMVEAPIFHVNGDDPEAVCYVMQAAMEFRMQFKKDVV
                     IDLVCFRKLGHNEGDDPMLTQPMMYKKIAKHPGVRAKYAERLVHEGVVSAEEADGLIR
                     AYRDALDKGEHVEHTVLSNYTRAHKLDWTPFLGTHWAHPTDTTLPANDIKRLADKVTS
                     VPEGFKLHPTVDKVLAARRLMGAGEQPVDWGMAETLAYASLVENGYGVRISGEDSGRG
                     TFSHRHAVLHDQNRERWDQGNYVPLRHLSENQAEFLVIDSILNEEAVLAYEYGYACSA
                     PDQLVIWEAQFGDFANGAQVAIDQFIVSGETKWGRLCGLTMILPHGYDGQGPEHSSAR
                     VERYLQLCAEHNIQVVMPSTAAQMFHMMRRQMLRPYRKPLVIMMSKRLLRLKDSLSPL
                     SELTSSSFKPVIGDVTELDAKKVKRVVVCAGQVYYDLFNARKEAGNDELAIVRLEQLY
                     PFPTEQLAAELAKYPNARELMWAQEEPKNQGAWYQIRHRLEKLLSPKQTLLFAGRPSS
                     ASPAVGYMSKHVAQLKSLLAEAVAK"
     gene            4544..>4758
                     /locus_tag="PKN55_00920"
     CDS             4544..>4758
                     /locus_tag="PKN55_00920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014086290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide succinyltransferase"
                     /protein_id="HNX69835.1"
                     /translation="MLIEIKIPVFAESVTEGTIAALHKKVGDAVARDENLADIETDKI
                     MLELPAPQAGVLVELTVAVGDTVTSQQV"
     assembly_gap    4759..4858
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4859..5743
                     /gene="odhB"
                     /locus_tag="PKN55_00925"
     CDS             <4859..5743
                     /gene="odhB"
                     /locus_tag="PKN55_00925"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014086290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HNX69836.1"
                     /translation="ETLAMPAAKKLAAETGVDLAGVAGSGRDGRVLKEDVAAAAAKPA
                     AAPAKPAAAPVVALPVGDRPEQRVPMTRLRKVVAERLVQSQQTNAILTTFNEVNMKPL
                     MDLRNLYKDKFEKAHGVKLGFMGFFVKAAVHALKKYPIINASVDGTDIVYHGYFDIGV
                     AVGSPRGLVVPVIRNADLLSLADIEKQIADFGKRAQEGKLGLDELTGGTYTISNGGTF
                     GSMMSTPIINPPQSAILGMHSTKERAVVENGQIVARPMMYLAQSYDHRIIDGREAVLS
                     LVAIKEAIEDPARLILDL"
     gene            5840..7270
                     /gene="lpdA"
                     /locus_tag="PKN55_00930"
     CDS             5840..7270
                     /gene="lpdA"
                     /locus_tag="PKN55_00930"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003745605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HNX69837.1"
                     /translation="MSQSFDVAVIGGGPGGYVAAIRAAQLGFNTVCIDAFKNPQGKPS
                     LGGTCLNVGCIPSKALLQSSENYHAIQHDFAAHGITAENPKIDIAKMLERKDGIITKN
                     AGGIAFLFKKNKVANVHGLGKLVGRQNEKWLIEVTDGGETQQIEATHVIVATGSNPRP
                     LPGVEVDNVRVLDNAGALAMTEVPARLGVIGAGVIGLEMGSVWKRLGAEVTVLEAMPT
                     FLAAVDEQIAKEAHKYLTKDTGLVIHNGVKIGAIENGADAVTVKYELNGQAQELVVDK
                     LIVAIGRVPNTAGLNAEGVGLQIDERGFIVVDDHCHTNLPNVWAIGDVVRGPMLAHKA
                     SDEGVAVAERIAGQKPHLDFNTIPWVIYTHPEIAWVGKTEQQLKADGVDYKKGTSGFA
                     ANGRALGLGMTQGTVKVLACAKTDRILGVHIIGPMASELVAEAVAAMEFSASSEDIAR
                     IVHAHPSLSEVVHEACLAADKRALHG"
     gene            7345..8511
                     /gene="sucC"
                     /locus_tag="PKN55_00935"
     CDS             7345..8511
                     /gene="sucC"
                     /locus_tag="PKN55_00935"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HNX69838.1"
                     /translation="MNLHEYQAKELLAKYGLPVQQGVLATNGEEAAAAFDKLGGKFAV
                     IKAQVHAGGRGKAGGVKVVKSREEAADLAASLIGKNLVTYQTDANGQPVNSVLVCEDM
                     YPVQRELYLGAVVDRSSRRVTFMASTEGGVEIEEVAHNTPEKILKVTVDPLVGLLPFQ
                     ARDVAFQLGLEGKQINEFVKLMTGAYQAFVENDFALFEINPLAIRGDGSLACVDAKVG
                     IDSNALYRLPAVAALRDKSQENERELKASEFDLNYVALEGNIGCMVNGAGLAMATMDI
                     IKLKGGQPANFLDVGGGATKERVIEAFKLILADPSVQGVLINIFGGIVRCDMIAEAII
                     AAVKEVNVTVPVVVRLEGNNAEAGAKLLDESGLKLTAAQGLNDAAEKIVAAVNA"
     gene            8600..9487
                     /gene="sucD"
                     /locus_tag="PKN55_00940"
     CDS             8600..9487
                     /gene="sucD"
                     /locus_tag="PKN55_00940"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010327286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HNX69839.1"
                     /translation="MSVLVNKNTKVLVQGFTGKNGTFHSEQALAYGTQVVGGVTPGKG
                     GSQHLGLPVFNTMKEAVRETQADASVIYVPAPFVLDSIVEAVDAGIKLIVTITEGVPT
                     LDMLKAKRYIEQTGGVRLIGPNCPGIITPGECKIGIMPGHIHKPGKIGIVSRSGTLTY
                     EAVAQTTAAGLGQSTCIGIGGDPIPGTSHIDALELFQNDPETEAIIMIGEIGGTAEEE
                     AAEFAKSNVTKPIVGYIAGVTAPKGKRMGHAGAIISGGKGTAEEKFAAFEKAGIRYTR
                     SPAELGSTLVALLKEKGML"
     gene            complement(9652..10923)
                     /locus_tag="PKN55_00945"
     CDS             complement(9652..10923)
                     /locus_tag="PKN55_00945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018150464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNX69840.1"
                     /translation="MKLETIAVHGGYTPDPTTKAVAVPVYQTASYAFDSAQHGADLFD
                     LKVQGNIYTRIMNPTTDVLEKRVAELEGGIAGLALASGMAAILYSIQTIAESGDNIIA
                     TSTLYGGTYNLFAHTLPQLGIEVRFVDYRDPQAVSALVDERTKAVYCESVGNPLGNVV
                     DFAAFADVAHAAGVPLIVDNTVPSPYLCRPFEHGADIVVHSLTKYLGGHGNSIGGIIV
                     DSGKFPWGEHAERFARLNTPDVSYHGVNYVEALGPAAYIARARVVPLRNMGATISPFN
                     SFLILQGIETLALRMDRICENAQRVAEHLASHPAVSWVEYAGLADNASKPLVDKYMGG
                     RASGILSFGVKSGREGGARFLDALKLVTRLVNIGDAKSLATHPASTTHRQLNDEELAK
                     AGVKPDMVRLSIGIEHIDDILADIEQALAAA"
     gene            complement(11348..13468)
                     /locus_tag="PKN55_00950"
     CDS             complement(11348..13468)
                     /locus_tag="PKN55_00950"
                     /inference="COORDINATES: protein motif:HMM:NF012863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HNX69841.1"
                     /translation="MQRNPLARACALAMVLGAAPALAAPVYFFGDSLTDTGAFQGLAN
                     GSATLATGTRWTYDNAPFYADVLARALGGAAVANNPLNPATSSQGNNYAQGGARSSVQ
                     SSNTGPWSTAGAGGFPPADPRSASNLAVQDLPDQVGFYLSARNGRADSAAWHVLWSGG
                     NDIPAALETSAVSGSAAGVAQVSSDASSLLGQVQRLRVAGAERFIVPNLPAFGLTPAT
                     VSSTVLAVINAQPTLAAIPSSTKLAAIGAAQQVLSSAATPDAASQQALRRKALNAAES
                     VLGLPANTLVNQYEGSNGVQSSVNSLATLFNGTVNLGLSQTGVNLVRLDVAGLLNEII
                     ADPLRYGFDNVTGSACPAGVSSLACFSSTPGFNANLRYLFADDRHPSPQAQQMIGDYA
                     VATLAAPYYAAALPAAAESASAGLYNTLEQRHRLARQAPQTVGTVSAFAQYGYQPFAA
                     KGAGGLDGGGQQHAYTVGVETQLRAETRLGLALGYQQGTLDSGANSDFRFRQQRLAAY
                     ASQQWGEFSLAADAFIASNAYRSVRRSFALGAVSRSESGETDGEQTGLRVSGRYHWQY
                     GDWTLSPMLGLAYENIRVNGYGETGDNSTAMRFGSQKQAGWQARLGVDVATRLGRWLP
                     YASAEWVQRLSTRQATLDAAIKSQPGQFSTELARPERSWGQLRLGSQVELVKGLMGNI
                     EAYSSVARNSGRQWGVQLGVSHDF"
     gene            complement(13642..14385)
                     /locus_tag="PKN55_00955"
     CDS             complement(13642..14385)
                     /locus_tag="PKN55_00955"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HNX69842.1"
                     /translation="MIFALTPAWAADVGPRPLVRLAVSEWPPFTGEHLPGNGIGADTL
                     RQMLGRAGYQLELVWLPWTRAVRDGSGMDSKLDGYFPEYTTADTRRRCQLSAPIGMSV
                     LGLAHQAAQPVNWTHMADLARYRVGVVNGYVNTDAFDAAVSAGILRVDGASSDESNLR
                     KLLRERIDVAVIDREVMGYLLAEPALRNQAGQLAFHPHWLGELPLRVCFRHGARLQPI
                     LAAMQAHRLPAAQLRAGQRDYLRQLAEQR"
     gene            complement(14538..14858)
                     /gene="grxD"
                     /locus_tag="PKN55_00960"
     CDS             complement(14538..14858)
                     /gene="grxD"
                     /locus_tag="PKN55_00960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003824637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Grx4 family monothiol glutaredoxin"
                     /protein_id="HNX69843.1"
                     /translation="MSANIQDVIKQQVTDNPVLLYMKGSAQFPQCGFSARAVQLLKTC
                     GVSKLATVDVLQDPDIRQGIKEFSNWPTIPQLYINGEFIGGSDIMAQMYESGELQELL
                     AKVA"
     gene            complement(15030..15485)
                     /gene="fliW"
                     /locus_tag="PKN55_00965"
     CDS             complement(15030..15485)
                     /gene="fliW"
                     /locus_tag="PKN55_00965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008953694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliW"
                     /protein_id="HNX69844.1"
                     /translation="MLFQSNLLGQVTVDENTVLNFPNGLPGFESCQRFKLFHEESDAP
                     RVFWMQSLDDADVLFSVVTPEEFGVRYEVELSAEQVDALQLAKPEDATVLVMIYKGNQ
                     EIDANTHPLLKSLSANLRNPLVINTATLRGVQICQLECDMLLHNRKAGE"
     gene            complement(15612..16469)
                     /locus_tag="PKN55_00970"
     CDS             complement(15612..16469)
                     /locus_tag="PKN55_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008952348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HNX69845.1"
                     /translation="MSSFISREVEHLVPGQPASDGAGVRLLRVLSGAGWQRRLDPFLM
                     LDEFRSDQPGDYLAGFPEHPHRGFQTVTYMLAGRMRHRDSVGHEGVIGPGAVQWMNAG
                     RGILHSEMPEQQHGLMHGFQLWINLPASRKLSAPAYRELEADQIPELRTASGSLIRVI
                     AGQLDGVDGAIQEADTEPQYWDVRLPAGACEYLALPPGHHAAVYVYAGEAQLGEQARP
                     TAARTLAVLSQTEGSHGVIVRATQAARLLVLAGRPLHQPIAQWGPFVMNTRAEIEQAI
                     ADMRAGTLA"
     gene            16673..17587
                     /locus_tag="PKN55_00975"
     CDS             16673..17587
                     /locus_tag="PKN55_00975"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HNX69846.1"
                     /translation="MPERLPPLDVLPGFVMVGRTLSMTRAAQALCLSQSAVSRQIRQL
                     EAAVGQPLFLREHRALRLTDAGARLLLSAEAALRAWQDGVAALRDDAARQITVSASIG
                     VIGLWLLPRLGDWQAAHPQLDMRLLADNRKREPRAAGVDLAIRYDAADALPADALPLF
                     DETLAVVASPQHGPAALSQPAALADATLLTFDSPAHPWLSWPHWLAAQGWRVAPRMLH
                     FNQYDQLIHAALAGQGLALGRLPLLAPWLANGRLRRIPAPAGPVCRHRYALLRAAEPT
                     SPAVAQAVEWVLSQAAQTRRTLQALASD"
     gene            complement(17535..18527)
                     /locus_tag="PKN55_00980"
     CDS             complement(17535..18527)
                     /locus_tag="PKN55_00980"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNX69847.1"
                     /translation="MSVPLALSFPAADGYVLHGHVWAHAQPCASRPVVVVNPATAVLS
                     RYYARFAAYLHAAGCEVLTYDYRGIGGSRPARLRGFRAGWIEWGSLDFEGALREVQRR
                     FAGQPILVAAHSAGGLALGLAPSNHLVTRAFTMGAQFAHWRDYAPGQRLGMWWKWHVL
                     MPALTACLGYFPGRRLGWMEDAPAGVVEDWTARAPRFEAVARRGGERVTARMRAQWLT
                     QCAQPRADLLALSISDDPFAGDAAIERLLAYFTGCRRWRLHVRPAQIGVPAIGHFAFF
                     HDRFADTLWPIARDWLLHGACPPEFAATPWPGETPPALSRSPAPAMCAASAPPG"
     gene            18657..18953
                     /locus_tag="PKN55_00985"
     CDS             18657..18953
                     /locus_tag="PKN55_00985"
                     /inference="COORDINATES: protein motif:HMM:NF013488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HNX69848.1"
                     /translation="MLQAPVTPLEFWFDLASPYSYLAAARLPALAAAHGVVVVCWHRA
                     RADAAQPAVGQALRARIHQAEARGLFGAPSFWVAGELFWGNDRLEEAVEWAVRR"
     gene            complement(19099..19239)
                     /locus_tag="PKN55_00990"
     CDS             complement(19099..19239)
                     /locus_tag="PKN55_00990"
                     /inference="COORDINATES: protein motif:HMM:NF021526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2256 domain-containing protein"
                     /protein_id="HNX69849.1"
                     /translation="MPRRLAKADLPSKICPVCQRPFSWRRKWAKDWPQVKYCSERCRR
                     AG"
     gene            complement(19248..20045)
                     /gene="folE2"
                     /locus_tag="PKN55_00995"
     CDS             complement(19248..20045)
                     /gene="folE2"
                     /locus_tag="PKN55_00995"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: protein motif:HMM:NF010200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003933 - GTP cyclohydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0035998 - 7,8-dihydroneopterin
                     3'-triphosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase FolE2"
                     /protein_id="HNX69850.1"
                     /translation="MGAPETLPDVQGMLADFGFTIDQVGVKSVKFPLQVASADGGAQA
                     TVATLDMYVALPPTQKGTHMSRFLEQLHAHQGPLDGRALHGFVRDMAQRLNAESAEVS
                     LRFPYFVRKTAPVSGAVSLLDVEAAMRGEWRADRGWTLTLTVTTPVTSLCPCSKEISR
                     YGAHNQRSAITLSVELAADADMSLEHLVRVAEHSASCELFGLLKRPDEKWVTERAYDN
                     PKFVEDLVRDIARALVADPRVQAFVVESENFESIHNHSAFARIRHRR"
     gene            complement(20078..20635)
                     /locus_tag="PKN55_01000"
     CDS             complement(20078..20635)
                     /locus_tag="PKN55_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019924025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HNX69851.1"
                     /translation="MLRPLYCLSALWLALSAGLAQAADCPPLLKHRFTTLQGQPLDLC
                     AQQDKVILVVNTASKCGYTRQFEPLEALSRRWRDQGLLVIGFPSNDFNQELASNQEVA
                     EFCKLTYFVDFPMIEKAKVSGAQAMPFYRQLAAATGQAPRWNFHKYVIAPGGKQVWAF
                     ASEVEPDSPQLLKVIQPYLRTAERR"
     gene            20822..>21717
                     /locus_tag="PKN55_01005"
     CDS             20822..>21717
                     /locus_tag="PKN55_01005"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HNX69852.1"
                     /translation="MNSRIGFDWRDLEVFVAAAESGSIARAAERCRLAPSAASKRLSD
                     LEAALGAPLLERHNRGVRPTAAGQALLQRSRGLLEEARRIEREVRSYGEGLSGHVRLF
                     ANISAIVEYLPAALAQFAKAWPGIRVELEEHVSSEVAQAVAENVADVGVVSDLSDFDG
                     VALHPCWRDQLALLAPAGHPLAHAARVRFAQALEHPMVGLKPGSTLHSRLLRAAAELG
                     RSLPMQVQVGSFDAMCAMIAAGMGVGIMPRAASLPYLDALRLTATPLDESWAEHQLYL
                     CRKADGELSPAAARLFAHLAAQA"
BASE COUNT         4099 a         7116 c         6771 g         3631 t
ORIGIN      
        1 aagacgcccc gtccgtgtgg tgcggacagg ggcgccctta aaaccctacc tgggagtatt
       61 gctgtggaaa cgaatcgcaa agtgaccctt tcctacgacg agggcaaaac cctggatatg
      121 ccggtgatgt ccggcaccct gggcccggac gtcgtggaca tccgcacctt cgccaaaacc
      181 ggcatgttca cctttgaccc tggcttcctg gcgactgccg cctgcgaatc cagcatcacc
      241 ttcatcgacg gtgacctggg tcaactgtac taccgcggct acccgatcga gcaactggcc
      301 gagcactccg acttcctgga aagctgctac ctgctgctga acggcgaact gccgaccgct
      361 gcacagttcg ctgaattcaa ccgcaagatc atgcgccaca acatgctgaa cgaccagatc
      421 atgtcgttct tcaagggctt ccgccgcgac gcccacccga tggccgtgat ggtgggtgtg
      481 gtcggcgcgc tgtccgcctt ctaccatgat tcgctggaca tcaacgacgc acaccaccgc
      541 gaagtgtcgg cccaccgcct gatcgccaag gtgccgaaca tcgccgccca ggcctatcgc
      601 tacaacaagg gcctgccgtt cagctacccg aagaacggtc tgacctactc cgagaacttc
      661 ctgcacatga tgttctcgac cccgtgcgaa gagtacaagg tcaacaaggt gctggccaag
      721 gcgctggatc gcatcttcat cctgcacgct gaccacgaac agaacgcctc gacctcgacc
      781 gtgcgtctgg ccggttcgtc gggcgccaac ccgtttgcat gtatcgccgc cggcatcgcc
      841 tgcctgtggg gcccgtccca cggcggcgcc aacgaagccg tgctgaaaat gctggacgaa
      901 atcggctcgg tggaaaacgt gccggccttc atggaaggcg tcaaggccaa gacccacaag
      961 ctgatgggct ttggtcaccg cgtgtacaag aacttcgacc cgcgcgccaa gatcatgaag
     1021 gaaacctgcg acgaggtgct gaacgaactg ggtctgcgcg acgatccgaa gttccagctg
     1081 gccatgaagc tggaagaaat cgccctgagc gacccgtact tcatcgagcg caagctgtac
     1141 ccgaacgtgg acttctactc cggcatcgtg ctgtccgcca tcggcatccc ggtgtccatg
     1201 ttcaccccga tcttcgccct ggctcgcacc gtgggctgga tcagccactg gaccgaaatg
     1261 atctccgatc cgggcatgaa gattggccgt ccgcgtcagc tgtacaccgg ttcggtgcgc
     1321 cgcgactacg cgcaagtggc cgatcgcaag taattcagcc atgcgcctga gccatcaggc
     1381 aaaccgctgg ctgtcgcccc ctggggcggc ggctggcgcg ttgagcagta tcccgccccg
     1441 caaggggctg gccgctggcg gcgcaggccg ccagttcttc agtcgcagca aaaaatataa
     1501 aaaatgaggc acgaaacgct ctcgggcgcc ggctgcccag aaacattgag acaacgccgg
     1561 cgagccacca ggcgcgcttc gtacccgctg cacgggcagt caacaaaggg tcgaccacac
     1621 atcatgatgc aaaccttgca aagtcattcc tacctgtttg gcgggaatgc accgtttatc
     1681 gaggaactgt acgaacagta ccttgccgac gccaacgccg tgcctggcga atggcgtgaa
     1741 tattttgaca aactccagca acaaggcggc gtggcgcgcg atgtgccgca ccagccgatt
     1801 caggagtcgt tcatccagtt ggccaagcag ccgcgcattg gcgccgcgcc cagcgccagt
     1861 gcggacggcg cctcgctgca aaagcaggtg gcggtgctca agctgatttc ggcttaccgc
     1921 gtgctgggct cgcgctgggc caatctggat ccgctcaagc gcatggatca atcgccggtg
     1981 cgcgagctgg acccgtccac ccacggcctg tccgacgccg acatggcggt gcaattcaac
     2041 accggctcgc tggttggcca gcagaaaaag ctgccgctgt cggaaatcct gaaaaacctc
     2101 aagcaaacgt actgcggcaa tatcggcctg gagtacatgc acatcaccaa ttccgaacaa
     2161 aagcactgga tcatgcagcg ctttgaagcg gatttgtcca ccccgcgttt cggcaaggac
     2221 gccaaaaagc gcatcctcaa gcaagtgacc gccgccgaaa ccctggaacg ctacctgcac
     2281 accaagtacg tcggccagaa gcgcttctcg ctggaaggcg gcgaatcgat gattgccgcg
     2341 ctggacaacc tgatccagaa ctcctcgtcg attggcgtgc aagaaatcat catcggcatg
     2401 gcccaccgtg gccgtctgaa cgtgctggtg aacaccctgg gcaagctgcc gcgcgacctg
     2461 ttcgccgaat tcgaaggccg cccggcggtg gaactgccgt ccggcgacgt gaagtaccac
     2521 atgggcttct cgtccgacat ccccaccgcc aatggcccgg tgcacgtgtc gctggcgttc
     2581 aacccctcgc acctggaaat cgtcaacccg gtggtggaag gctcggtgcg cgcgcgccag
     2641 caacgccgtg gcgaccacga gcgccgtcaa gtggtgccgg tgctgatcca cggtgacgcc
     2701 gccttcggcg gcctgggcgt caaccagggc accttcaacc tgtcgggcac ccgtggctac
     2761 ggcaccggcg gcacgctgca tctggtggtc aacaaccagg tgggcttcac cacctccgac
     2821 acccgcgaca tgcgctccac gctgtattgc accgacgtgg ccaaaatggt ggaagccccg
     2881 atcttccacg tgaatggcga cgatccggaa gccgtctgct atgtgatgca ggcggccatg
     2941 gaattccgca tgcagttcaa gaaagacgtg gtcatcgacc tcgtctgctt ccgcaagctg
     3001 ggccacaacg agggcgatga cccgatgttg acccagccga tgatgtacaa aaaaatcgcc
     3061 aagcacccag gcgtgcgcgc caagtatgcc gagcgtctgg tgcacgaagg tgtggtgtcc
     3121 gccgaagaag ccgatggtct gattcgcgcc taccgcgatg cgctggacaa gggcgagcac
     3181 gttgaacaca ccgtgctgag caactacacc cgcgcccaca agctggactg gacgccgttc
     3241 ctgggcaccc actgggcgca cccgaccgac accaccctgc cggccaacga catcaagcgc
     3301 ctggccgaca aggtcaccag cgtgccggaa ggcttcaagc tgcacccgac cgtggacaag
     3361 gtgctggccg cccgccgcct gatgggcgct ggcgaacagc cggtggactg gggcatggcg
     3421 gaaaccctgg cctatgcctc gctggtggaa aacggctacg gcgtgcgcat ctccggcgaa
     3481 gactccggcc gtggcacctt cagccatcgc cacgccgtgc tgcacgacca aaaccgtgag
     3541 cgctgggacc aaggcaacta cgtgccgctg cgccatctgt cggaaaacca ggctgaattc
     3601 ctggtgatcg actcgatcct gaacgaagaa gccgtgctgg cctacgaata tggctacgcc
     3661 tgctcggccc cggatcagct ggtgatctgg gaagcccagt ttggcgactt cgccaacggc
     3721 gcccaggtgg cgattgacca gttcatcgtc tctggcgaaa ccaaatgggg ccgtctgtgc
     3781 ggcctgacca tgattctgcc gcacggctac gacggccagg gcccggaaca ctcgtccgcc
     3841 cgcgtcgagc gctacctgca actgtgcgcc gagcacaaca tccaggtggt gatgccgtcc
     3901 accgccgcgc agatgttcca catgatgcgc cgtcagatgc tgcgtccgta ccgcaagccg
     3961 ctggtcatca tgatgtccaa gcgtctcttg cgcctgaagg attcgctctc cccgctgtcc
     4021 gaactgacct ccagcagctt caagccggtg atcggcgatg tgaccgagct ggacgccaag
     4081 aaggtcaagc gcgtggtggt gtgcgccggt caggtctatt acgatctgtt caacgcccgc
     4141 aaagaagccg gcaatgacga gctggccatt gttcgcctgg aacagctgta cccgttcccg
     4201 accgagcaac tggctgccga actggccaag tacccgaacg cccgtgaact gatgtgggcg
     4261 caggaagagc cgaagaacca aggcgcctgg taccagatcc gtcatcgtct ggaaaaactg
     4321 ctgtcgccca agcaaaccct gctgttcgcc ggccgtccgt cgtccgcttc gccggccgtg
     4381 ggttacatgt ccaagcacgt cgcccagctc aagtcgctgc tggccgaggc cgtggccaag
     4441 taatcccgcg cccggccgcc cccgtggcgg ccgggcacat gcattgcaca agaccccatt
     4501 tgaacgggct gcccgccggg cggcccactg aggagaccct cacatgctga tcgaaatcaa
     4561 aatcccggtg ttcgccgaat ccgtgaccga aggcaccatc gccgccctgc acaaaaaagt
     4621 gggcgacgcc gtggcccgcg atgaaaacct ggccgacatc gaaaccgaca agatcatgct
     4681 ggaactgccg gccccgcaag ccggcgtgct ggttgagctg accgtggccg tgggcgacac
     4741 cgtcaccagc caacaggtnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnga
     4861 aaccctggcc atgccggccg ccaagaaact ggccgctgaa accggcgtgg atctggctgg
     4921 cgtggcaggc tctggccgcg atggccgcgt gctgaaagaa gacgtcgccg ctgccgccgc
     4981 caagccggct gccgccccgg ccaagcccgc cgctgcgccg gtggtggccc tgccggtcgg
     5041 cgaccgtccg gaacagcgcg tgccgatgac ccgcctgcgc aaggtggtgg ccgagcgtct
     5101 ggtgcagtcg caacagacca acgccatcct gaccaccttc aacgaagtca acatgaagcc
     5161 gttgatggac ctgcgcaacc tgtacaaaga caagttcgaa aaagcccacg gcgtcaagct
     5221 gggctttatg ggcttctttg tgaaggccgc cgtgcacgcc ctgaaaaagt acccgatcat
     5281 caacgcctcg gtggacggca ccgacatcgt gtaccacggc tactttgaca tcggcgtggc
     5341 cgtgggcagc ccgcgtggcc tggtggtgcc ggtgatccgc aacgccgacc tgctgagcct
     5401 ggccgacatc gagaagcaaa tcgccgactt cggcaagcgc gctcaagaag gcaagctggg
     5461 cctggacgag ctgaccggcg gcacctacac catctccaac ggcggcacct ttggctcgat
     5521 gatgtccacc ccgatcatca acccgccgca atccgccatc ctcggcatgc actccaccaa
     5581 ggagcgcgcc gtggtggaaa acggccagat cgtggctcgc ccgatgatgt acctggcgca
     5641 atcctacgac caccgcatca tcgacggccg cgaagccgtg ctgagcctgg tggccatcaa
     5701 ggaagccatt gaagatccgg cccgcctgat cctcgacctg taatcatccg cgttgcggcg
     5761 cgctggccct gtccgggccg agcgcgccgt tgccatatcc ggagccaggc cacaagcctg
     5821 cacaccagga gcacaccgaa tgagccaaag ctttgacgtc gccgtgatcg gcggcggccc
     5881 cggcggctat gttgccgcca tccgcgccgc ccaactgggc tttaacaccg tctgcatcga
     5941 cgccttcaag aacccgcaag gcaaacccag cctgggcggc acctgcctga acgtgggctg
     6001 catcccgtcc aaggcgctgc tgcaatcctc ggaaaactac cacgccatcc agcacgactt
     6061 tgccgcccac ggcatcaccg ccgaaaaccc gaagatcgac atcgccaaga tgctcgaacg
     6121 caaggacggc atcatcacca aaaacgcggg cggcattgcc tttttgttca aaaagaacaa
     6181 agtcgccaat gtgcacggcc tgggcaagct ggtgggtcgc cagaacgaaa aatggctgat
     6241 cgaagtcact gacggcggcg aaacccagca aatcgaagcc acccacgtca tcgtcgccac
     6301 cggctccaac ccgcgtccgc tgccgggcgt ggaagtggac aatgtgcgcg tgctggacaa
     6361 cgctggcgcg ctggccatga ccgaggtgcc ggcccgcctg ggcgtgattg gcgcgggcgt
     6421 gatcggcctg gaaatgggca gcgtgtggaa gcgcctgggc gctgaagtca ccgtgctgga
     6481 agccatgccg accttcctgg ccgccgtgga cgagcaaatc gccaaagaag cgcacaaata
     6541 cctgaccaag gacaccggcc tggtgatcca caatggcgtg aaaatcggcg cgattgaaaa
     6601 cggcgccgac gccgtcaccg tcaagtacga actcaacggc caagcgcaag agctggtggt
     6661 ggacaagctg attgtcgcca tcggccgcgt gcccaacacc gccggcctga acgccgaagg
     6721 cgtgggcctg caaatcgacg agcgcggctt tatcgtggtg gacgaccact gccacaccaa
     6781 tctgcccaat gtctgggcca ttggcgacgt ggtgcgcggc ccgatgctgg cgcacaaggc
     6841 cagcgatgaa ggcgtggccg tggccgagcg cattgccggg caaaagccgc acctggactt
     6901 caacaccatt ccgtgggtga tctacaccca cccggaaatc gcctgggtgg gcaagaccga
     6961 gcaacagctc aaggccgacg gcgtggacta caaaaaaggc acgtccggct ttgccgccaa
     7021 cggccgcgcg ctgggcctgg gcatgaccca aggcacggtg aaggtgctgg cctgcgccaa
     7081 gaccgaccgc atcctgggcg tgcacatcat cggcccgatg gcttccgaac tggtcgccga
     7141 agccgtggcc gccatggaat tctccgccag cagcgaagac atcgcccgca tcgtccatgc
     7201 ccacccgagc ctgtcggaag tggtgcacga agcctgcctg gccgctgaca agcgcgccct
     7261 gcacggctga tgatttttca gtgaagcgcg ccgccaaccc cggcgcgcct cacgcctcgc
     7321 ccctgaaaaa agagagacag acacatgaac ctgcacgaat accaagcaaa agaattgctg
     7381 gccaaatacg gcctgccggt gcaacaaggc gtcctcgcca ccaacggcga agaagcagcc
     7441 gctgctttcg acaagctggg cggcaagttt gccgtgatca aggcccaggt gcatgccggc
     7501 ggccgcggca aggccggtgg cgtgaaggtg gtgaagagcc gcgaggaagc cgctgacctg
     7561 gctgccagcc tgatcggcaa gaacctggtg acctaccaga ccgacgccaa tggccagccg
     7621 gtgaacagcg tgctggtgtg tgaagacatg tacccggtgc agcgcgaact gtacctgggc
     7681 gccgtggtgg atcgctcctc ccgtcgcgtg accttcatgg cttccaccga aggcggcgtg
     7741 gaaatcgaag aagtcgccca caacaccccg gaaaaaatcc tgaaagtcac cgtggatccg
     7801 ctggtcggcc tgctgccgtt ccaggcgcgt gacgtggcgt tccagctggg cctggaaggc
     7861 aagcaaatca acgaattcgt caagctgatg accggcgctt accaagcctt cgtcgaaaac
     7921 gacttcgccc tgttcgaaat caacccgctg gccatccgtg gcgacggcag cctggcctgc
     7981 gtggacgcca aggtcggcat cgactccaac gccctgtacc gcctgccggc cgtggccgcg
     8041 ctgcgcgaca agtcgcaaga aaacgagcgc gagctgaagg ccagcgaatt cgacctcaac
     8101 tacgtggcgc tggaaggcaa tatcggctgc atggtgaacg gcgccggtct ggccatggcc
     8161 accatggaca tcatcaagct caagggcggc caaccggcca acttcctgga cgtgggcggc
     8221 ggcgccacca aggagcgcgt gatcgaagcc ttcaagctga tcctggctga tccgtcggtg
     8281 caaggcgtgc tgatcaacat cttcggcggc atcgtgcgct gcgacatgat cgccgaggcc
     8341 atcattgccg ccgtcaagga agtcaacgtc accgtgccgg tggtggtgcg cctggaaggc
     8401 aacaacgccg aagccggcgc caagctgctg gacgagtccg gtctgaagct gaccgccgcc
     8461 cagggcctga acgacgccgc cgagaaaatc gtcgccgccg tcaacgcctg atgtggagcg
     8521 aggccgacgg cgacgggcgt gcgccggcta ccgcctcctc ctccgttgac acgcctgaca
     8581 cccagttttg aggttacaaa tgagcgtact cgttaacaag aacaccaaag tcctcgttca
     8641 aggctttacc ggcaagaacg gcaccttcca ctccgaacag gctctggcct acggcaccca
     8701 ggttgtgggc ggcgtgaccc cgggcaaggg cggcagccag catctgggcc tgccggtgtt
     8761 caacaccatg aaagaagccg tgcgcgaaac ccaggccgac gcctcggtga tctacgtgcc
     8821 ggccccgttc gtgctggatt ccatcgttga agccgtggac gccggcatca agctgatcgt
     8881 caccatcacc gaaggcgtgc cgaccctgga catgctcaag gccaagcgct acatcgagca
     8941 aaccggcggc gtgcgcctga tcggcccgaa ctgccctggc atcatcaccc cgggcgagtg
     9001 caagatcggc atcatgccgg gccacatcca caagccgggc aagatcggca tcgtgtcccg
     9061 ctccggcacc ctgacctacg aagccgtggc gcaaaccacc gctgccggcc tgggccaatc
     9121 cacttgcatc ggcattggcg gcgacccgat tccgggcacc agccacatcg acgcgctgga
     9181 actgttccag aacgacccgg aaaccgaagc catcatcatg atcggtgaaa tcggcggcac
     9241 cgccgaagaa gaagccgccg agttcgccaa gtccaatgtg accaagccga tcgtcggtta
     9301 catcgctggc gtgaccgcgc cgaagggcaa gcgcatgggc cacgctggcg ccatcatctc
     9361 cggcggcaag ggcaccgctg aagaaaagtt tgccgcgttc gaaaaagccg gcatccgcta
     9421 cacccgcagc ccggccgaac tgggcagcac cctggtggcc ctgctgaaag aaaaaggcat
     9481 gctgtaagcc tggtgcgcac gccggctgct gccggcgtca gccatgaaaa agccctgcgg
     9541 atttcgcagg gctttttttc tggcgggagt gcggcttagt gacgctaaga aaaaccgcca
     9601 tacgggcacg ctgtatggcg gtttttgggc ggggagagtc acgccaggct tttacgcagc
     9661 cgccagcgcc tgctcgatgt cggccagaat atcgtcgatg tgctcaatgc caatcgacag
     9721 gcgcaccatg tccggcttga cgccggcctt ggccagttct tcgtcgttca gctggcggtg
     9781 ggtggtgctg gccgggtggg tggccaggct cttggcgtcg ccgatattga ccaggcgggt
     9841 caccagcttg agcgcatcca ggaagcgcgc gccgccttcg cggccggatt tcacgccaaa
     9901 cgacaagatg ccagaggcgc ggccgcccat gtatttgtcc accagcggct tgctggcgtt
     9961 gtcggccagg ccggcgtatt ccacccagct cacagccggg tggctggcca ggtgctcggc
    10021 cacgcgctgg gcgttttcgc agatgcggtc catgcgcagc gccagggttt cgatgccttg
    10081 caggatcaaa aagctgttga acggcgagat ggtcgcgccc atattgcgca gcggcaccac
    10141 gcgggcgcgg gcgatgtagg cggccgggcc cagcgcttcg acgtagttga cgccgtggta
    10201 gctgacatcc ggggtgttca ggcgggcaaa gcgctcggcg tgctcgcccc aggggaattt
    10261 gcccgaatcg acaatgatgc cgccgatgct gttgccgtgg ccgcccaggt atttggtcag
    10321 cgaatgcacc acgatgtcgg cgccgtgttc gaacgggcgg cacagatacg gcgagggtac
    10381 ggtgttgtcc acaatcagcg gcacgccagc ggcgtgggcc acgtcggcaa aggcggcaaa
    10441 gtccaccaca ttgcccagcg gattgcccac cgattcgcag tacaccgcct tggtgcgctc
    10501 atcgaccagc gcgctcaccg cctgcgggtc gcggtaatcg acaaagcgca cttcgatgcc
    10561 cagctgcggc agggtgtggg caaacagatt ataggtgccg ccatacaggg tgctggtggc
    10621 gatgatattg tcgcccgatt cggcgatggt ctggatcgaa tacagaatgg ccgccatgcc
    10681 cgaggccagc gccagcccgg caatcccgcc ttccagctcg gccacgcgct tttccagcac
    10741 gtcggtggtc gggttcatga tgcgggtgta gatattgccc tgtaccttca ggtcgaacag
    10801 gtcggcgccg tgttgggcgc tgtcgaaggc gtagctggcg gtctggtaca ccggcacggc
    10861 cacggccttg gtggtcgggt cgggggtata gccgccgtgg acggcgatgg tttccagttt
    10921 catgcggtct ggctcctcta ttttgtgatg aaacgacttg gcgaagttac ccggaatggc
    10981 cacatgctgc aatagtgcat ggcaatatca ttagcacttg ttgatctgca gcgccgaggt
    11041 gggtaagggc tggctggcag gggttccgga ttgtggcgcc ggctttcgca tcactcacaa
    11101 aatcctgctc accttcctgg ccagaacccg cttcgttgtg tctgctgggc cacgctaagc
    11161 ggctgggaga tgggccgtgg atgctttgaa cgccggggcg tgtgtgattg gtggacacgg
    11221 agtgatcctg accctgatat atggctatgc gtcgggtgcg ggaaaggcat ggcacagggg
    11281 cgggtgggtt gtgcccgccc ctgtgagagg gggtgtcgct tgatcggggt agagccccga
    11341 cttccgctca aaagtcgtgg cttaccccca gttgtacccc ccattgccgg ccgctgttgc
    11401 gcgccacgct gctgtaggcc tcaatattac ccatcaggcc tttgaccagt tctacctggc
    11461 tgcccaggcg cagctgcccc caactgcgtt ccgggcgggc cagctcggtg ctgaactggc
    11521 ctggctggct cttgatggcc gcgtccagcg tagcctgacg ggtggacagg cgctgcaccc
    11581 attcggcgct ggcatacggc agccagcggc ccaggcgggt ggccacgtcc acgcccagcc
    11641 gggcttgcca gcctgcctgc ttctggctgc caaagcgcat ggcagtgctg ttgtcgccgg
    11701 tttcaccgta gccattcacc cggatgtttt cataagccag gcccagcatc gggctgagcg
    11761 tccagtcgcc gtattgccag tgataacggc cgctcacacg cagaccggtt tgttcaccgt
    11821 cggtttcccc gctctcgcta cggctgaccg cgcccagggc aaagctgcgg cgcactgagc
    11881 gataggcatt gctggcgata aacgcatctg ccgccagtga gaattcgccc cactgctggc
    11941 tggcgtaagc agccaggcgt tgctggcgaa agcgaaaatc actgttggcg ccgctgtcca
    12001 gcgtgccttg ctgatagccc agcgccaggc ccaatcgcgt ttcggcgcgc agctgggttt
    12061 ccacccccac ggtataggcg tgttgctgac cgccgccatc cagtccgcct gcgcccttgg
    12121 cggcaaacgg ctggtagccg tattgggcaa aggcgctgac cgtgcccacg gtttgcgggg
    12181 cttgccgcgc caggcggtgg cgttgctcca gcgtgttata cagcccggcg ctggcggatt
    12241 ctgccgctgc cggcagcgcc gcagcgtaat acggcgcggc cagtgtcgcc acggcatagt
    12301 cgccaatcat ctgctgcgcc tgcggcgagg ggtggcggtc atcggcaaac aaatagcgca
    12361 aattggcgtt aaagcccggt gtggaagaaa aacacgccag cgacgacaca ccggccgggc
    12421 aggccgagcc ggtgacattg tcaaagccat agcgtagtgg gtcggcgatg atttcattca
    12481 gcaggccagc cacatccagc cgcaccagat tcaccccggt ctggctcagt cccagattga
    12541 ccgtgccatt aaacagcgtg gccagcgaat tcacgctgct ttgcacgcca ttgctgccct
    12601 cgtattggtt caccagagta ttggccggca gacccagcac gctttcggcg gcattgagcg
    12661 ctttgcggcg tagcgcttgc tggctggccg cgtcgggcgt ggcggcgctg gacagcacct
    12721 gttgtgctgc gccaatggcc gccagcttgg tgctggacgg aatggccgcc agggtgggct
    12781 gggcgttgat caccgccagc acggtggacg ataccgtggc cggggtcagg ccaaaggcgg
    12841 gcagattggg cacaataaag cgctcggccc cagccactcg caggcgctgt acctgaccca
    12901 gcagactgct ggcgtcactg ctgacctggg ccacacctgc ggcgctgcca ctgacggcac
    12961 tggtttccag cgcggcgggg atgtcattgc cgccactcca cagcacatgc caggcggcgc
    13021 tgtcggcgcg gccattgcgc gctgacagat aaaaccccac ttggtcgggc aaatcctgca
    13081 cggccagatt gctggccgag cgtgggtcgg ccggcggaaa acccccggcg cctgcggtgc
    13141 tccacggtcc ggtgttgctg gactgcacgc tggagcgcgc gccgccctgg gcgtagttat
    13201 tgccctgtga tgaggtggcc gggttcagtg gattattggc cacggcggcg cctcccaggg
    13261 cgcgggccag cacgtcggcg taaaacggcg cgttgtcgta ggtccagcgt gtgccagtgg
    13321 ccaaggtggc cgagccattg gccaggcctt gaaatgcgcc agtgtcggtc aggctgtcac
    13381 cgaaaaaata taccggcgca gccagcgccg gggcggcgcc cagcaccatg gccagcgcgc
    13441 aggcgcgggc gagcggattg cgttgcatga gatctcctcg tgttgaagac aggcggcgcg
    13501 ggcgtcttgg cgccctgcgt ccctggcctg ccgggatggc gggccaggcg tcgcatcatc
    13561 gacgacgcgg agataaaata aacgtttaaa tccggcgggg aaagcttttc tgatattgca
    13621 ttgcaatatg gtgcatcggg ctcagcgttg ctcagccagc tggcgcagat agtcacgctg
    13681 accggcgcgt aactgggcgg ctggcaggcg gtgcgcctgc atggcggcca ggatgggttg
    13741 cagacgcgcg ccgtggcgaa agcacacccg caagggcagc tcgcccagcc agtgcggatg
    13801 aaacgccaac tgtccggcct gattgcgcaa ggccggctcg gccagcagat agcccatgac
    13861 ctcacgatca atcaccgcca cgtcgatgcg ctcgcgcaat aacttgcgca gattgctctc
    13921 atcgctgctg gcgccatcca cccgcaatat gccggcgctg acggcggcat caaaggcatc
    13981 cgtgttgaca tagccattga ccacgcccac ccggtaacgg gccagatccg ccatgtgggt
    14041 ccagttcacc ggctgcgctg cctggtgcgc caggcccagc acactcatgc cgatcggtgc
    14101 gctcaactga caacgccgac gggtatccgc cgtggtgtat tccggaaaat acccatccag
    14161 cttgctgtcc atgcccgagc catcgcgcac cgcccgtgtc catggcagcc agaccagctc
    14221 cagctgataa ccggcccgcc cgagcatctg gcgcagggta tccgcaccaa tgccattgcc
    14281 cggcaggtgc tcgccggtaa agggaggcca ttccgagacg gccaggcgca ccagcgggcg
    14341 cgggccaacg tcggcggccc aggccggcgt cagcgcaaaa atcatcccaa tccagctcaa
    14401 tcgccacagg caaacaaggt tacgcatggt ctcaggcccg aaaaaacgat gcatgccagt
    14461 ttgacacacc ttgcacggca cagacatgac aacgccccga caggcggggc gtggacaaca
    14521 acatcaacac actacactca ggcgactttg gccagcagct cttgcagctc accagactca
    14581 tacatctgcg ccatgatgtc cgagccgccg atgaattcgc cgttgatgta cagctgcgga
    14641 atggtcggcc agttggagaa ctccttgatg ccctggcgga tgtccgggtc ttgcagcaca
    14701 tccacggtgg ccagcttgct gacgccacag gttttcagca gctgcacggc gcgcgccgaa
    14761 aaaccgcact gcggaaactg ggccgagccc ttcatgtaca ggagcaccgg attgtcggtg
    14821 acttgttgct tgatgacgtc ttgaatattg gcggacatgc tgaatccttt gcgggtcggt
    14881 gaggggcagg caataatacc cgagtaattt gttaggattt taatccgctg ctttgacagg
    14941 gtcaatccgg tatggcgccg catcctggcc tgcacacaac aacgccacgc agatagcgtg
    15001 gcgttgttat ggttactgtg gctgcggact cactcgcctg ccttgcggtt gtgcagcagc
    15061 atgtcgcatt ccagctgaca gatctgcacc ccccgcaggg tggcggtatt gatcaccagc
    15121 ggattgcgca gattggcgct caaggacttc agcagcgggt gggtgttggc gtcgatctcc
    15181 tggttgccct tgtagatcat caccagcacg gtggcgtctt ccggcttggc cagttgcagc
    15241 gcatccactt gttcggcgct gagctcgact tcgtagcgca cgccaaactc ttccggcgtc
    15301 accacgctga acagcacatc ggcgtcgtcc agcgattgca tccagaacac gcgcggcgcg
    15361 tcggattctt catggaacag cttgaagcgc tggcaggatt cgaaccctgg cagcccattc
    15421 gggaaattca ggacggtgtt ttcatcaaca gtgacttgtc cgagcaggtt cgattggaac
    15481 agcattataa gactcctccg cagggcggat gacatggaaa catgaagtat tctggcatag
    15541 tcattctgtg ccagctcttg cggcaagtca aagcccatcc acatcgttcg ccctgtcgtg
    15601 cagagagagg atcacgccag cgtgccggcc cgcatatcgg caattgcctg ctcaatctcg
    15661 gcgcgagtgt tcatcacaaa cggcccccac tgggcaatcg gctggtgaag cggccgcccg
    15721 gccagcacca aaaggcgcgc cgcctgtgtt gcacggacaa tcactccgtg gctaccttcg
    15781 gtctggctga gcactgccag cgtgcgcgcc gccgttggac gggcttgctc acccaactgc
    15841 gcctcgcccg cgtagacata caccgccgcg tgatggccag gcggcagcgc caggtattcg
    15901 caagcgccgg ccggcaggcg tacatcccaa tactgcggct cggtatccgc ttcttgaatg
    15961 gccccatcca caccgtccaa ctgcccggca atcacccgga tcaggctgcc actggcggtg
    16021 cgcagctcgg gaatttgatc cgcctccagc tcgcgatacg ccggcgcgct cagcttgcgg
    16081 ctggccggca ggttgatcca cagctgaaag ccatgcatca gcccatgctg ctgctcaggc
    16141 atttcggaat gcaggatgcc gcgcccggcg ttcatccact gcaccgcccc cggaccaatc
    16201 accccctcgt ggccgacact gtcgcggtga cgcatgcgcc cggccagcat ataggtgacg
    16261 gtctgaaatc cccggtgcgg gtgctcggga aagccggcca gataatcccc cggctgatcc
    16321 gagcgaaact cgtccagcat caggaagggg tcgagccggc gctgccagcc cgcgccgctg
    16381 agtacacgca acaagcgcac cccggcgcca tcagaggccg gctggccagg gacaagatgc
    16441 tcgacttctc ggctgatgaa agaggacatg gcacagactc cagtacaggc ttgcttcaag
    16501 ggatccgacc aagagcaaac cgggttgaat tgatgatctg cactggattt taggtgcgtc
    16561 gcccagatca agatagcgag gattttgcgg aggtttgttc agaaaatccg aacctttgcc
    16621 catgcctgcc atatggcaca atctcctgcc attctcctgc tggtcaccca ccatgcccga
    16681 acgcctgccc ccactggatg tcttgcccgg ttttgtcatg gttgggcgca cgctgagcat
    16741 gacccgcgcc gctcaggcgc tgtgtctgag ccaatccgcc gtcagccggc aaatccgcca
    16801 gctggaagcc gccgttggcc agcccttgtt tctgcgcgaa caccgcgcgc tgcggctgac
    16861 cgatgccggc gcgcggctgt tgctcagcgc cgaagccgcc ctgcgcgcct ggcaagacgg
    16921 ggtggcggcg ctgcgcgacg acgccgcccg gcaaatcacc gtcagcgcca gcattggcgt
    16981 gattggcctg tggctgttgc cacggctggg cgactggcag gccgcgcacc cgcagctgga
    17041 catgcgcctg ctggccgaca accgcaagcg cgagccgcgc gccgccgggg tggacctggc
    17101 catccgctac gacgccgccg acgccttgcc ggccgacgcg ctgccgctgt ttgacgaaac
    17161 cctggccgtg gtggccagcc cccagcacgg ccccgccgcg ctcagccagc cggccgcgct
    17221 ggccgacgcc accttgctga ccttcgacag cccggcccac ccctggctgt cctggccgca
    17281 ttggctggcg gcgcagggtt ggcgcgtggc gccgcgcatg ctgcatttca atcaatacga
    17341 ccagctgatt cacgccgcgc tggccggcca gggcctggcg ctgggccggc tgccgctgct
    17401 ggccccatgg ctggccaatg gccgtctgcg ccgcatcccg gcgccggctg gcccggtttg
    17461 ccgtcatcgc tacgcgctat tgcgcgccgc cgagccgacc agcccggcgg tggcgcaggc
    17521 ggtggaatgg gtgcttagcc aggcggcgca gacgcggcgc acattgcagg cgctggcgag
    17581 cgactgagcg caggtggcgt ctcgcccggc cagggcgttg cggcaaactc cggcgggcaa
    17641 gcgccgtgca atagccagtc gcgggcaatc ggccacagcg tgtcggcaaa gcgatcatga
    17701 aaaaacgcaa aatggccgat ggccggcacg ccgatctgcg ccgggcgcac atgcagccgc
    17761 cagcgtcggc agccggtaaa ataggccagc aaacgctcaa tggcggcgtc gccggcaaag
    17821 ggatcatcgc tgatcgacaa ggccagcaaa tcggcgcgcg gctgcgcgca ctgggtgagc
    17881 cactgcgcgc gcatgcgcgc cgtcacccgc tcgccgccgc gccgcgccac cgcctcaaaa
    17941 cgcggcgccc gcgccgtcca gtcctccacc accccggccg gcgcatcctc catccagccc
    18001 aaccgccgcc ccggaaaata ccccaaacac gcggtcagcg ccggcatcag cacatgccac
    18061 ttccaccaca tgcccagccg ctggcccggc gcataatcgc gccaatgggc aaactgcgcg
    18121 cccatggtga acgcccgcgt caccagatga ttggacggcg ccagccccag cgccaggccg
    18181 ccggcgctgt gcgccgccac caaaatcggc tgcccggcaa agcgccgctg cacttcacgc
    18241 aaggcgcctt caaaatccag gctgccccac tcgatccagc cggcgcgaaa gccgcgcaag
    18301 cgggctgggc gcgagccgcc aatgccgcga taatcataag tcagcacctc gcagccagcg
    18361 gcgtgcagat aggcggcaaa gcgcgcgtaa taacggctga gcaccgccgt ggccggattg
    18421 acgaccacca ccggccggct ggcgcacggc tgggcgtgcg cccagacatg gccgtgcaac
    18481 acatagccat cggcggccgg aaagctcagg gcaagcggaa cagacatgac acacactcca
    18541 gcaacatcat cacgccccca ctgtagccag ccggcgcgcc cgcttcaaac gggttttttg
    18601 cgcgcttcgc atgcgtggcg cgcatgcgaa aacatccgct ccgcaggctg aaccccatgc
    18661 tccaagcacc cgtcacccca ttggaattct ggttcgactt ggccagcccc tacagctatc
    18721 tggccgccgc ccgcctgccg gcgctggctg ctgcgcacgg cgtcgtggtg gtctgctggc
    18781 accgcgcccg cgccgacgcc gcccagccgg cggtgggcca agcgctgcgc gcacgcattc
    18841 accaagccga agcgcgcggc ctgttcggcg cgcccagctt ctgggtggcg ggggaattgt
    18901 tctgggggaa tgaccggctg gaggaggcgg tggagtgggc ggtgcggcgg tgagacggcg
    18961 caactatgtt ctgagctgag ggtgggcagc gccaagcatt gttcaccaca ctcagcgctg
    19021 cgcgtctgct actgggcgcg cgcagccagt gcgaggccct gccacaaggc cagcaggctt
    19081 tggtgagcgg ggctcagcct accccgcccg ccggcagcgc tcgctgcaat acttcacctg
    19141 cggccagtct ttcgcccatt tgcgccgcca gctgaacggg cgttggcaga ccgggcagat
    19201 tttgctgggc aagtcagctt tggccaggcg gcggggcatg gggcggctca gcggcggtgg
    19261 cggatgcggg cgaaggcgga gtggttgtgg atggattcga agttttcgct ttccaccaca
    19321 aaggcctgca cgcgcgggtc ggccaccagg gcgcgggcga tgtcgcgcac caggtcttcc
    19381 acaaacttcg ggttgtcgta ggcgcgttcg gtcacccatt tttcgtccgg gcgcttgagc
    19441 aggccaaaca gctcgcacga ggcgctatgc tcggccaccc gcaccagatg ctccaggctc
    19501 atgtcggcgt cggcggccag ctccacgctc agggtgatgg ccgagcgctg gttgtgcgcg
    19561 ccatagcggg agatttcttt cgagcacggg cacaggctgg tgaccggggt ggtgacggtc
    19621 agcgtcagcg tccagccacg gtcggcgcgc cattcgccgc gcatggcggc ttctacgtcc
    19681 agcaggctga ccgcgccaga caccggcgcg gttttgcgca caaaatacgg aaagcgcagg
    19741 ctgacttcgg cggactccgc gttcagccgc tgcgccatgt cgcgcacaaa gccatgcagc
    19801 gcccgcccgt ccagcgggcc ctggtgcgcg tgcaattgct cgagaaagcg cgacatatgc
    19861 gtgccctttt gcgtgggcgg cagcgccaca tacatatcca gcgtggccac ggtggcttgc
    19921 gcgccaccgt cggcgctggc cacttgcagg gggaatttca ccgatttgac gcccacctga
    19981 tcgatggtga agccaaaatc agccagcatg ccttgtacat cgggcagggt ttcgggtgcg
    20041 cccatggcgt gtctccgggg ggaagaaaaa tcaaaactca gcggcgctcg gccgtgcgta
    20101 aataaggctg gatcactttc agcagctgcg gcgaatcggg ctcgacctca gaggcaaacg
    20161 cccacacctg cttgccgccg ggggcaatca catatttatg gaagttccag cgcggcgcct
    20221 ggccggtggc ggccgccagc tggcgataaa acggcatggc ctgcgcgccg ctgaccttgg
    20281 ccttttcgat catcgggaag tcaacaaaat aggtcagctt gcagaactcg gccacttcct
    20341 gattgctggc cagctcctgg ttaaaatcat tgctgggaaa gccaatcacc aacaaaccct
    20401 gatcgcgcca gcgccgcgac agcgcctcca gcggctcaaa ctggcgggtg tagccgcatt
    20461 tgctggcggt gttgaccacc agaatcactt tgtcctgctg cgcgcacaaa tccagcggct
    20521 ggccttgcag tgtggtgaag cggtgcttga gcaacggcgg gcagtcggcg gcctgagcca
    20581 ggccggcgct caaggccagc cagagcgcgc taaggcaata cagcggacgc aacatgacag
    20641 gttctcccgg cgcatgcgcc aggcgcgcgc caaatggtcg gcaatcgatt caacatagaa
    20701 tcggccccct gggtttgtca atgttttgta ctggacaaaa tcgtttcgcc tcgcgcaatg
    20761 cggcaaacag cccttgccgg gcgctacaat cggcgtattg agtgtttttc tggtgcgcat
    20821 gatgaattcc cgcattggtt ttgactggcg cgacctggaa gtctttgtcg ccgccgccga
    20881 aagcggcagc attgcccgcg ccgccgagcg ctgccggctg gcgccctccg ccgccagcaa
    20941 gcggctgtcc gacctggagg ccgcgctggg cgcgccgctg ctggagcggc acaaccgtgg
    21001 cgtgcgcccc accgctgccg gacaggccct gttgcagcgt tcgcgcggcc tgctggaaga
    21061 agcccggcgc atcgagcggg aggtgcgcag ctatggcgaa ggcctgagcg gccatgtgcg
    21121 cttgttcgcc aatatctcgg cgattgtcga atacctgccg gcggcgctgg cgcagtttgc
    21181 caaggcctgg ccgggcattc gcgtggaatt ggaagaacat gtgtccagcg aggtggcgca
    21241 ggcggtggcg gaaaacgtcg ccgatgtggg cgtggtcagc gacctgagcg actttgacgg
    21301 cgtggcgctg catccgtgct ggcgcgacca actggccctg ctcgcccccg ccggccaccc
    21361 gctggcccac gccgcgcgcg tgcgctttgc ccaggcgctg gaacacccga tggtgggcct
    21421 caagcccggc agcaccctgc acagccggct attgcgcgcc gccgccgaat tgggccgcag
    21481 cctgcccatg caagtgcagg tgggcagctt tgacgccatg tgcgccatga tcgccgccgg
    21541 catgggcgtg ggcatcatgc cgcgcgccgc ctcgctgccc tatctggatg ccctgcgcct
    21601 gaccgccacg ccgctggatg aaagctgggc cgagcatcag ctgtatctat gccgcaaagc
    21661 cgacggcgag ctttctcctg ctgcggcgcg gctgtttgcg cacctggcgg cgcaggc
//
LOCUS       DAOPYX010000009        21397 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_2518_length_21397_cov_11.302362, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000009 DAOPYX010000000
VERSION     DAOPYX010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 21397)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21397)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21397
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2518_length_21397_cov_11.302362"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(5..1132)
                     /locus_tag="PKN55_01010"
     CDS             complement(5..1132)
                     /locus_tag="PKN55_01010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009120076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HNX69853.1"
                     /translation="MSAPAIVITGSGLFTPPHRISNEELVTAYNAWAEADNARHAEAI
                     AAGQRAARELSSAEFIVKASGIHSRHVMDRDGVLNPARMCPNLPERDNDSLSLQAEMA
                     VAAGRDALAAAGRAAAEIDMVIVACSNMQRAYPAMAIEVQQALGCGGFAYDMNVACSS
                     ATFGMQAAVSALSSGAAKAVLMVNPEITSGHLNFCDRDSHFIFGDACTAVVLERADTA
                     RSEQGFEVLGCKLATQFSNNIRNNFGFLNRAAPEGIGQPDKLFVQQGRKVFKEVCPMV
                     AEHIEAHLASLQLRASDIRRYWLHQANLSMNQLIARRLLGRDASLDEAPVILDRYANT
                     SSAGSVIAWHLHQQDLPSGALGVLASFGAGYSVGSVVLRKR"
     gene            1375..1851
                     /gene="iscR"
                     /locus_tag="PKN55_01015"
     CDS             1375..1851
                     /gene="iscR"
                     /locus_tag="PKN55_01015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011134650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HNX69854.1"
                     /translation="MRLTTKGRFAVTAMLDLAMRDANGPVTLAGISERQGISLSYLEQ
                     LFGKLRRAELVESVRGPGGGYCLARLVEDITVADIIIAVDEPLDATQCGGHENCHDNH
                     RCMTHDLWTDLNKTIFAYLANVTLASLVDKQRAKETAVVQDRRRVAAVQDGAAPAL"
     gene            1885..3108
                     /locus_tag="PKN55_01020"
     CDS             1885..3108
                     /locus_tag="PKN55_01020"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005666322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0044571 - [2Fe-2S] cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IscS subfamily cysteine desulfurase"
                     /protein_id="HNX69855.1"
                     /translation="MTAPAKNYPIYLDYSATTPVDPRVAQAMIPWLTEHFGNPASRSH
                     AYGWESEEAVENARAEVAKLVNCDPKEIVWTSGATESNNLAIKGAAQFYKTKGKHLIT
                     VKTEHKAVLDTCRELERQGFEVTYLTPQDNGLLDLDAFRAAIRPDTILASVMFVNNEI
                     GVIQDIAALGDICRERGVIFHVDSAQATGKVEIDLASLKVDLMSFSAHKTYGPKGIGA
                     LYVRRKPRIRLEAQIHGGGHERGMRSGTLATHQIVGMGEAFRLAREEMASENARIGAL
                     RDRLWAGLSDIEEVYLNGDLDRRVPHNLNVSFNFVEGESLIMAIKELAVSSGSACTSA
                     SLEPSYVLRALGRNDELAHSSIRFTIGRFTTEAEVDFAIHLLKAKIGKLRELSPLWDM
                     FKEGVDLNTVQWAAH"
     gene            3258..3641
                     /gene="iscU"
                     /locus_tag="PKN55_01025"
     CDS             3258..3641
                     /gene="iscU"
                     /locus_tag="PKN55_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HNX69856.1"
                     /translation="MAYSEQVLDHYENPRNVGSFDKGDGTVGTGMVGAPACGDVMKLQ
                     IKVNEAGVIEDAKFKTYGCGSAIASSSLVTEWVKGKTLDEALAIKNTQIAEELALPPV
                     KIHCSILAEDAIKAAVSDYKAKHGH"
     gene            3694..4017
                     /gene="iscA"
                     /locus_tag="PKN55_01030"
     CDS             3694..4017
                     /gene="iscA"
                     /locus_tag="PKN55_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017508212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HNX69857.1"
                     /translation="MGITLTERAAEHVQGFLSKRGKGLGLRLGVKTSGCSGMAYKLEF
                     VDEAGEEDLSFESHGVTLFTDAKSLAYIDGTELDFVKEGLNEGFKFNNPNVKNECGCG
                     ESFNV"
     gene            4038..4565
                     /gene="hscB"
                     /locus_tag="PKN55_01035"
     CDS             4038..4565
                     /gene="hscB"
                     /locus_tag="PKN55_01035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008952564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0051259 - protein complex oligomerization
                     [Evidence IEA];
                     GO_process: GO:0097428 - protein maturation by iron-sulfur
                     cluster transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly co-chaperone HscB"
                     /protein_id="HNX69858.1"
                     /translation="MDFNQDFFTLFQLPRRFALDRAALDAAYRRIQGEVHPDRFAASP
                     EAERRLALQWATRANEAHQTLRQPLARARYLLALAGVDTAEDTNTAMPAEFLMLQMEW
                     REAMMDARAARDADALETLIREVRADARALEAELAVALDDSGDFAAAASLVRKLRFLE
                     KLEADLGEALEALLF"
     gene            4636..6495
                     /gene="hscA"
                     /locus_tag="PKN55_01040"
     CDS             4636..6495
                     /gene="hscA"
                     /locus_tag="PKN55_01040"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014088290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HNX69859.1"
                     /translation="MALLQIAEPGLFAAPHQHRLAVGIDLGTTNSLVATVRSGAAVVL
                     PDEHGRHLLPSVVRYTPDGECVVGHAARARQNEDPHNTIVSAKRFMGRGLADLDNPAA
                     MPYQFVDAPGMVQLLTRAGVKSPVEISADILRALKARAEASLGGELVGAVITVPAYFD
                     DAQRQATKDAARLAGLNVLRLLNEPTAAAIAYGLDNASEGVYVVYDLGGGTFDVSILR
                     LSRGVFEVLATSGDSALGGDDFDHRIYCWVLEQAGLSQLSANDTRLLLTHARAAKEML
                     TDAQEAPIQATLSDGQLVQLTLTLETFEQITRTLVDKTLLPVRKALRDARLSVEDIQG
                     VVMVGGATRMPHIRKAVAEHFRQPPLTNLDPDQVVALGAAMQANVLAGNKGDDDWLLL
                     DVIPLSLGLETMGGLVEKIIPRNSTIPTARAQEFTTFKDGQTAMALHVLQGERELVAD
                     CRSLARFELRGIPPMVAGAARIRVTFQVDADGLLSVSAREQSSGVEARIEVKPSYGLT
                     DDEVSRMLTESIAHVHDDIAARKLAEATVDAEGLLAAIDVALASDGDLLDAAERSALD
                     AAIAAMRAALDARQTVGINQAMATLNEASGDFAARRMDRNIRRALTGQKISEL"
     gene            6548..6886
                     /gene="fdx"
                     /locus_tag="PKN55_01045"
     CDS             6548..6886
                     /gene="fdx"
                     /locus_tag="PKN55_01045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009425374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HNX69860.1"
                     /translation="MPKIIVLPHADLCPQGALFDAEPGLSICDNLLNNQVDIEHACEM
                     SCACTTCHVVIREGFASLEPSDELEDDLLDKAWGLEATSRLSCQALVTDTDLVVEIPR
                     YTINHAKENH"
     gene            6888..7082
                     /gene="iscX"
                     /locus_tag="PKN55_01050"
     CDS             6888..7082
                     /gene="iscX"
                     /locus_tag="PKN55_01050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003812452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HNX69861.1"
                     /translation="MQWTDSLDIAIALCDAHPDIDPKTVRFTDLMQWVLALPEFNDDP
                     AHCGERILEAIQQAWIDEVE"
     gene            complement(7218..8048)
                     /locus_tag="PKN55_01055"
     CDS             complement(7218..8048)
                     /locus_tag="PKN55_01055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009311982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ScpA family protein"
                     /protein_id="HNX69862.1"
                     /translation="MSAPALLAADAPASAPPLATVFGEAVLDAPADLFIPPDALRVFL
                     ESFEGPLDLLLYLIRKQNLNVLDIPMARVTAQYMEYIGAMRAERMELAAEYLLMAALL
                     IEIKSRLLLPRPPRDEEGEVDDPRAELVRRLLEYEQIKLAALALDALPQAERDFAWLA
                     VLVEQSAEARLPEVSPADLRQAWMAILARAKHTRHHQVEQDSLSVREQMTRILRQLQG
                     RGHVPFVELFELDINVSYVVVNFIAILELAKEGLISITQDAPYQPIYVRLGREDASSE
                     "
     gene            complement(8080..>8613)
                     /locus_tag="PKN55_01060"
     CDS             complement(8080..>8613)
                     /locus_tag="PKN55_01060"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018151565.1"
                     /note="catalyzes a two-step reaction, first charging a
                     tryptophan molecule by linking its carboxyl group to the
                     alpha-phosphate of ATP, followed by transfer of the
                     aminoacyl-adenylate to its tRNA; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HNX69863.1"
                     /translation="NLSIGDRERLFGYLENKGKVILVEPQALLTEASRMPGLDGQKMS
                     KSYGNGIFLREDPAAVSKKVRGMPTDPARVRRTDPGSPDKCPVWQLHQVYTDRDTHDW
                     VQSGCRSAGIGCLECKQPVIDGILAEQQPMFERAQQYLDDPTLVKSIVADGCERARAI
                     AQETMRDVREAMGLGYA"
     assembly_gap    8614..8713
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<8714..9039)
                     /locus_tag="PKN55_01065"
     CDS             complement(<8714..9039)
                     /locus_tag="PKN55_01065"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012697572.1"
                     /note="catalyzes a two-step reaction, first charging a
                     tryptophan molecule by linking its carboxyl group to the
                     alpha-phosphate of ATP, followed by transfer of the
                     aminoacyl-adenylate to its tRNA; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HNX69864.1"
                     /translation="MYSERVLSGMRPTGSLHLGHYHGALKNWVKLQSEHECFFFVADW
                     HALTTSYDDPSIIAKSTWDMVIDWLAAGVDPSQATLFIQSRVPEHAELHLLLSMITPL
                     SWLERVP"
     gene            complement(9101..9739)
                     /locus_tag="PKN55_01070"
     CDS             complement(9101..9739)
                     /locus_tag="PKN55_01070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008954937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HNX69865.1"
                     /translation="MDELNLIQKISVFALPVIFAITGHEAAHAYAARHFGDNTAYLEG
                     RMTLNPLRHIDWIGTVLLPLVCVAVGGFIFGWAKPVPVRFGNLRHPKRDMLWVSAAGP
                     GANLLMAVLWALMFKLALGMNNDYTEPLALMAQAGMLINVMLMALNLLPIPPLDGGRM
                     LMSVLPNRQAWQLSRLEPYGMWILIALMFTGLLSLLMNPLIKACYRFLNALL"
     gene            complement(9912..10538)
                     /locus_tag="PKN55_01075"
     CDS             complement(9912..10538)
                     /locus_tag="PKN55_01075"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018151563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HNX69866.1"
                     /translation="MSQLFHIHPENPQARLIREAVKIIQQGGVIAYPTDSCYALGCHL
                     GDKRAMERILAIRQLDLRHHLTLVCRDLSELGSYARVDNAQYRLLKAITPGAYTFILQ
                     ATKEVPRRTLHPKRNTIGLRVPDHPVALALLEALGEPLLSCTLMLPHEPMPPSDPFEI
                     RDLLQSQLDLVIDGGYCGIEPTTVLDLTDGAPQLIRAGAGPLDKLGLA"
     gene            complement(10547..11410)
                     /locus_tag="PKN55_01080"
     CDS             complement(10547..11410)
                     /locus_tag="PKN55_01080"
                     /EC_number="3.1.3.97"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018747482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097657 - 3',5'-nucleotide bisphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-nucleoside bisphosphate phosphatase"
                     /protein_id="HNX69867.1"
                     /translation="MAKLDLHCHSTASDGALPPAELVARAAARGSRMLALTDHDCTAG
                     LAEAGEAAREHGLDLIRGVEISVSWRTRTIHIVGLDIDPDCPALAAGLRAVREGRVER
                     ARRMEAALAKIGIHGAFDGAMALCGNPEMIGRTHFARFLVESGEVKNVATVFRKYLVK
                     GKPGYVPHQWAELADAVGWIRAAGGVAVIAHPGRYDIGRQLQEELVQDFKAVGGEAIE
                     VVSASHTLDQTHKFALLAARMELLASAGSDFHAPGEGGRDVGLTQDLPPVCVPVWSRF
                     TPPRATPVAAN"
     gene            complement(11438..11656)
                     /locus_tag="PKN55_01085"
     CDS             complement(11438..11656)
                     /locus_tag="PKN55_01085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008954934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase-like domain-containing protein"
                     /protein_id="HNX69868.1"
                     /translation="MSQPDDVFDPEPEAPEPPADGACCGSGCDNCVLDVYQAQLSDYR
                     LKHWQWTQRQTASSEAERNAPAARQASW"
     gene            complement(11653..11868)
                     /locus_tag="PKN55_01090"
     CDS             complement(11653..11868)
                     /locus_tag="PKN55_01090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011135243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HNX69869.1"
                     /translation="MQQDFTLTGEYIALNDLLKICGVADSGGAGKALVASGAVSVDGA
                     QELRKTCKIRAGQIVSGDGFTIRVVGA"
     gene            11993..12709
                     /locus_tag="PKN55_01095"
     CDS             11993..12709
                     /locus_tag="PKN55_01095"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HNX69870.1"
                     /translation="MKLLTLPWRAPRATLLPAAFYRRQWALLAVLAALFYAALGDGRL
                     DHWLAAFSFDPLSQHFPLQHNWWLEYVNHVWLKDVSIVIGVAALGLGVFGPRFGLDAE
                     AARRWRLACAAMVVGSLSVSLLKQASPYHCPWDLLEFGGHAPYVPLLASLPAGVSAGK
                     CFPAGHASGGFVLMALYFVYAGERPRLAQAWLWGGLAFGLLMGVGQQLRGAHFLSHTL
                     WSGWVVWAAMLAVNQLLGRR"
     gene            12807..15053
                     /locus_tag="PKN55_01100"
     CDS             12807..15053
                     /locus_tag="PKN55_01100"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNX69871.1"
                     /translation="MNPGSCLVDDPGVANLQLLSHAEQQDILLLQQAILESVAQGHDP
                     LDIINEVCCLEERLLPNAVGSVMLLDEDGKLNVYAAPSVPPEGVERLRLLCPGPNSGS
                     CGNALYRGEPVFVSDTLTDPRWADLRDLAVDFNLLACWSMPIYGAGREIIGTFALSSF
                     ERRAPTEFHRKLLEIGASIIGIVLERRREADNLRLMAKVFENSNEGILVTDAQARIVS
                     VNPAFVSMTGYSRDEALGQTPRLLASGIHDQVFYQHMWASLLEQGHWHGEIWNRRKNG
                     EVFPEWLSLSAVHDHNGVLSHYVGLFFDLSERKAAEARAEYLIHHDPLTDLPNRLQAK
                     NRLNEAIARRQDDGEGVALLYVDLDNFKTINDSLGQAMGDQLLKAAAQRMQTLLQSQE
                     MLARHGADEFLLMVPHVREPRQLTELASLLLNAFAAPFDINGQQLHSTLSIGVALAPD
                     DGDDYDALLQKAETAVRAAKEAGRNTFRFFTGQQGGQAAEQLLIRNGLHNALLRNEFI
                     LHYQPQLDLTTGRIVGAEALVRWLHPEQGMVPPGKFIPVAEESGLIVPIGEWVLHEAC
                     RQLAQWREEGLGDLVVAVNLSGVEFKRGNLVESVTHTLARTGLPATCLELELTESMLM
                     HDVENVISTLQRLKALGVRLSIDDFGTGYSCLSYLTRFDVDTLKIDQSFVRQLHLGDQ
                     HAAIIRAVIQMAHSLDLATIAEGVEDAEVVAFLRAHACDQAQGYFFSKPLPAEDFARF
                     VRQYRAVL"
     gene            15185..15258
                     /locus_tag="PKN55_01105"
     tRNA            15185..15258
                     /locus_tag="PKN55_01105"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15217..15219,aa:Gly,seq:ccc)
     gene            15343..17523
                     /locus_tag="PKN55_01110"
     CDS             15343..17523
                     /locus_tag="PKN55_01110"
                     /inference="COORDINATES: protein motif:HMM:NF017021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HNX69872.1"
                     /translation="MKWTSGLLRLTPYRLKMLGVLLALLVLVFITALSVFYARFDPEQ
                     VRMQLADALQTDGRTLSIGQISPAVFPRPGIKIDQISLSAPNASPDGDGPLFTLRRAE
                     IRLAWLPLLQAQKVVHAIKLDGVRLNLRREADGRWNFADLLEQKTASSLVVKLDSLKL
                     ADLNATVDDRVFKREFSLSEGSMEASGMLGNAQLSLAGLLKSGEHQAAVVLESPLNVA
                     EEQLTLDPFKLTATGLVAGLTDSRAELTGTVRVNLVSRQMTAENLQASLSSREPSVSL
                     TARMPSADWSQGKAGFQAPSLDFNAALNNKGSSYSFSGQLKDISGNWQALAATELTGA
                     MSWKAGEHTLTAQLKAPVTLSNWEIFRLTPVTVDTTLATPLLPRGQLKAAAKGELVAN
                     LARQQVTSKLNGQIDGQRFDFNARQDGFITPAHTVNLSLGKLDLNRYLAESTGNQVGL
                     FYGQRKFDFDWMNALNLSGDLRIAELTAGRFAMQQIGAKLSMRPEKLLLEDVTALIYG
                     GNLNGRLELTPGEQPKLVMFETLSGMRIRPLLRDLFDIDRLDGKGRLEATLTAEGQTF
                     TQLRKSLNGTVRVGLTEGALMGVDMVEAIKRLPTELARLTDDPSKRTDFQSLSAQFNF
                     SNGVARNHDLRLNSSLFSLQGHGKVDLVGNIIDYAMRVQPNILVFTNTKEMNVPLKIT
                     GALNAPTYALDFNALVKDKKTILEKQQVLKEQLSNQFNLFKTTP"
     gene            17617..18135
                     /locus_tag="PKN55_01115"
     CDS             17617..18135
                     /locus_tag="PKN55_01115"
                     /inference="COORDINATES: protein motif:HMM:NF024905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HNX69873.1"
                     /translation="MPSFIMSLSASLLFALKIALATLLAWPLLTWLGVEQPVWAVVTI
                     AAVSDPKLTATADLVRDRVSNTLIGCVVALLTVWTVGAHLGSLAAALAVSVVLARTID
                     HYPGSWRLTPITVAILLGSGIGLPASAALNLAGLRAAEIIVGSLLALGLAWAFSWWPH
                     RRWRNWRLARQG"
     gene            complement(18162..18374)
                     /locus_tag="PKN55_01120"
     CDS             complement(18162..18374)
                     /locus_tag="PKN55_01120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase family protein"
                     /protein_id="HNX69874.1"
                     /translation="MPYVNIKITREGASAEQKAALIAGVTALLRDTLGKNPATTVVVI
                     DEVDTDNWGIGGEAVTMLRARAAKPA"
     gene            complement(18435..19067)
                     /locus_tag="PKN55_01125"
     CDS             complement(18435..19067)
                     /locus_tag="PKN55_01125"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HNX69875.1"
                     /translation="MAYRLLMLDFDGVLADSFPFFRHTFNQLAAHHGFRPVAADELPA
                     LRRCGPRQIARHVGLPWWKLPRVARHFMQLMQAAPDSAPLFAGVADWLDAQLDAGQQL
                     ALVSSNSRANVTRSVGEARLARFAAVECGSALLGKAQRLRQVLRRLHIAPEHAAYLGD
                     QPADGDAAQCAGVDFIAVGWGYASLDGLRPCQPALTLTHPAELFSLLPPP"
     gene            complement(19099..19983)
                     /locus_tag="PKN55_01130"
     CDS             complement(19099..19983)
                     /locus_tag="PKN55_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011133617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNX69876.1"
                     /translation="MHAASRPPWQGYAAALGVVCIWTGFILISRWGGKSPLTGYDVLA
                     LRLGAASLLLLPFCRDLPRTVWRERRLWTLAAIGGVLYGVVVYTAFKYAPAAHGAILL
                     PGMQPFLVTALLWLALGERPTRSRLIGLTGIALGVLCVALPLLQAGDWRTLPGDALML
                     VASLTWAVYSVLVRQWAFSPWVLTRFVALGSALLYLPVYALWLPKGLAEASATQLITQ
                     ALYQGIGPTIIAMWLFLLAVQQLGAARTGALIGLVPVLAGLAAVPLLGEPLTASLLLG
                     LCAVSAGAWYAARPGRVA"
     gene            20083..20940
                     /locus_tag="PKN55_01135"
     CDS             20083..20940
                     /locus_tag="PKN55_01135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011133616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HNX69877.1"
                     /translation="MDLDDLRIFRAVVREGGITRAAARLHRVQSNVTTRIRQLEAHLG
                     VPLFVREGRRLSLAPAGAVLSDYAERLLELADQARAAVADAAPRGRLRLGSMESTAAA
                     RLPTRLAAFHQRYPDVQLELHTGATAPLLAQLRAGELDCVLVCGPVADARLCAEPAFM
                     ETLALIAPAGHPPITRARDVRPLTLLTFAPGCAYRHRLEQWLASDGVLPERVIELASY
                     HAMLGCVAAGMGIALAPLSLLDGMAASHAVSVHPLPAEHAPAPTLLVRRAGVDSPTVM
                     ALREVMLAG"
     gene            complement(21050..>21397)
                     /locus_tag="PKN55_01140"
     CDS             complement(21050..>21397)
                     /locus_tag="PKN55_01140"
                     /inference="COORDINATES: protein motif:HMM:NF025619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4351 domain-containing protein"
                     /protein_id="HNX69878.1"
                     /translation="LLRQSKHTLALPKVRDLKELKMTLAERFDQWAQQYEQRGKQEGL
                     QKGRQEGRQEGRQEGQQEGEARLLLRLLTRRFGELPVDAVTRIQRANLPQLEAWSDRV
                     LDARSLAEVFQPS"
BASE COUNT         3830 a         6886 c         6833 g         3748 t
ORIGIN      
        1 accctcaccg cttgcgcaac accacactcc ccaccgaata cccggcgcca aacgaagcga
       61 gcacgcccag cgcgccgctg ggcaaatcct gctggtgcag gtgccaggca atcaccgagc
      121 cggccgagct ggtgttggcg tagcggtcca gaatcactgg cgcttcgtcc aggctggcgt
      181 cgcggccaag caggcggcgg gcaatcagct ggttcatcga cagattggcc tggtgcagcc
      241 aatagcggcg gatgtcgctg gcgcgcagtt gcaggctggc caggtgcgct tcgatatgct
      301 cggccaccat cgggcagact tctttgaaca ccttgcggcc ctgctgcaca aacagcttgt
      361 ccggctggcc gatgccttct ggcgcggcgc ggttgagaaa gccaaagttg ttgcggatat
      421 tgttggaaaa ctgcgtcgcc agtttgcagc ccagcacttc aaaaccctgc tcgctgcgcg
      481 cggtatcggc gcgttccagc accacggcgg tgcaggcgtc gccaaaaata aagtggctgt
      541 cacggtcgca gaagttcaga tgcccggagg tgatttccgg gttgaccatc agcaccgcct
      601 tggccgcgcc gctggacagg gcgctgacag cggcctgcat gccaaaggtg gccgacgagc
      661 aggccacgtt catgtcgtag gcaaagccgc cgcagcccag cgcctgttgc acttcgatgg
      721 ccatggccgg ataggcgcgc tgcatattcg agcaggcgac aatcaccata tcaatctctg
      781 ccgccgcgcg tccggcggcg gccagcgcgt cgcgcccggc ggccaccgcc atttcggctt
      841 gcagcgacag gctgtcgttg tcgcgctcgg gcagattggg gcacatccgc gcggggttga
      901 gcacgccgtc gcggtccatc acatggcggc tgtggatgcc ggaggctttg acgatgaatt
      961 ctgctgacga cagctcccgc gcggcgcgct ggccggcggc aatcgcctcg gcgtggcggg
     1021 cattgtcggc ttcggcccag gcgttgtagg cggtgaccag ttcttcgttg ctgatgcggt
     1081 gcggcggcgt gaacaggccg gagccggtga tgacgatggc gggtgcgctc atggtggcgg
     1141 tttccttgcg atggcaacta tgcatgcgtt tgtttgaacg tgatgatagc gcatcgcgca
     1201 gatgaccggc agatgacagg cggcgcaggc agtatcaccc ccgcatttct acggagtgag
     1261 gggccagttc attcttgacc aaattactcg gatattatac ccttcaggca gtacccaaac
     1321 ccgctgaatt caccccactg cacacggccg ttgccggccc aggagacttt cgccatgcga
     1381 ctcaccacca aggggcgctt tgctgtcacc gccatgcttg acctcgccat gcgcgacgca
     1441 aatggcccgg tcaccctggc cggcatcagc gagcgccagg gcatttccct gtcttatctc
     1501 gaacagttgt ttggcaagct gcgccgcgcc gagttggtgg aaagcgtgcg cgggccgggc
     1561 ggcggctact gtctggcgcg gctggtggag gacatcaccg tggccgacat catcatcgcc
     1621 gtggacgagc cgctggacgc cacccagtgc ggcgggcatg aaaactgcca cgacaatcat
     1681 cgctgcatga cccacgatct gtggactgac ctgaacaaaa ccatttttgc ctacttggcc
     1741 aatgtgacgc tggcgagcct ggtggacaag caacgcgcca aagaaaccgc cgtcgtgcaa
     1801 gaccgccgcc gtgtggccgc cgtgcaggac ggcgccgcgc ccgccttgtg acacatcatt
     1861 gtccgcgctg gagaatctgc tgaaatgacc gcacccgcga aaaactaccc gatttacctg
     1921 gactactcgg ccaccacccc ggttgacccg cgcgtggccc aggccatgat tccctggctg
     1981 accgaacact ttggcaaccc cgccagccgc agccacgcct acggctggga gtcggaagaa
     2041 gccgtagaaa acgcccgcgc cgaagtggcc aagctggtga actgcgaccc gaaagaaatc
     2101 gtctggacgt ccggcgccac cgaatccaac aacctggcga tcaaaggcgc ggcccagttc
     2161 tacaaaacca agggcaagca cctgatcacc gtcaagaccg agcacaaggc cgtgctggac
     2221 acctgtcgcg agctggagcg ccagggcttc gaggtcacct acctgacccc gcaagacaac
     2281 ggcctgctgg atctggacgc cttccgcgcc gccatccgcc cggacaccat cctggcctcg
     2341 gtgatgtttg tgaacaacga aatcggcgtg attcaggaca tcgccgcgct gggcgacatc
     2401 tgccgcgagc gcggggtgat ctttcatgtc gattcggccc aggccaccgg caaggtcgag
     2461 attgacctcg ctagcctgaa agtggacctg atgagcttct cggcgcacaa gacctacggc
     2521 ccgaaaggca ttggcgcgct gtatgtgcgc cgcaagccgc gcattcgcct ggaagcgcaa
     2581 atccacggcg gcggccacga gcgcggcatg cgctcgggca cgctggccac ccaccagatt
     2641 gtcggcatgg gcgaagcctt ccgcctggcc cgtgaagaaa tggccagcga aaacgcccgc
     2701 atcggcgcgc tgcgcgaccg cctgtgggcc ggcctgtcgg atatcgaaga agtgtatctg
     2761 aacggcgatc tggaccgccg cgtgccgcac aatctgaatg tcagctttaa ttttgtcgaa
     2821 ggcgaaagcc tgatcatggc catcaaggag ctggcggtgt cgtccggctc ggcctgcacc
     2881 tcggcctcgc tggagccctc ttacgtgctg cgcgcgctgg gccgcaacga cgaactggcg
     2941 cacagctcga tccgcttcac catcggccgc ttcaccaccg aagccgaagt ggacttcgcc
     3001 atccatctgc tcaaggccaa gattggcaag ctgcgcgaac tgtcgccctt gtgggacatg
     3061 ttcaaggaag gcgtggacct gaacaccgtg cagtgggctg cgcactaagt cgcgccggca
     3121 tcaagcgtcg ctcaaaaaac gcagcgaagc gagcctggct aggcgtgcgg ccgcagacag
     3181 tacaactagt acgacaaggc cgcacaacga cgccaggcgc gttttgtgag cggcgctaaa
     3241 aacaaggaga accaaacatg gcatatagcg aacaagtcct cgaccactac gaaaacccgc
     3301 gcaatgtcgg ctcgttcgac aagggcgacg gcacggtggg caccggcatg gttggcgcgc
     3361 cggcctgtgg cgatgtgatg aaattgcaga tcaaggtcaa cgaagccggg gtgatcgaag
     3421 acgccaagtt caaaacctac ggctgtggct cggcgattgc ttcttcctcg ctggtgaccg
     3481 aatgggtgaa gggcaagacg ctggacgaag cgctggccat caagaacacc cagattgccg
     3541 aagagctggc cctgccgccg gtcaagatcc attgttcgat ccttgccgaa gacgccatca
     3601 aggcggcagt gtccgactac aaggccaaac acggccactg accctgcaaa ctgtccgggc
     3661 gcccagcgcc cggcctcact ggtggagagc atgatgggca ttaccctgac cgaacgcgcc
     3721 gcagaacatg tgcaaggctt cttgagcaaa cgcggcaaag gcctgggcct gcgcctgggg
     3781 gtgaaaacct ctggctgctc gggcatggcc tacaagctgg aatttgtcga tgaagccggc
     3841 gaagaagacc tgagctttga atcgcacggc gtgaccttgt tcaccgacgc caaaagcctg
     3901 gcctatatcg acggcaccga gctggatttc gtcaaggaag ggctgaacga gggcttcaag
     3961 ttcaacaacc ccaacgtcaa gaacgagtgc ggctgcggcg aaagcttcaa cgtctgaccc
     4021 ggcctcgcgg agcgcgcatg gacttcaatc aggatttctt caccctgttc cagctgcccc
     4081 ggcgctttgc gctggaccgc gccgcgctgg acgccgccta tcggcgcatt cagggtgaag
     4141 ttcaccccga ccgctttgcc gccagccccg aggccgagcg ccggctggcg ctgcaatggg
     4201 ccacccgcgc caatgaagcg catcaaaccc tgcgccagcc gctggcgcgc gcgcgctatc
     4261 tgctggcgct ggccggcgtg gacaccgccg aagacaccaa cacggcgatg ccggccgagt
     4321 ttttgatgct gcaaatggaa tggcgcgaag ccatgatgga cgcccgcgcc gcccgcgacg
     4381 ccgacgcgct ggaaaccctg atccgcgaag tgcgcgccga cgcccgcgcg ctggaggccg
     4441 agctggccgt cgcgctggac gacagcggcg actttgccgc cgccgccagc ctggtgcgca
     4501 agctgcgctt tctggaaaaa ctggaagccg acctgggcga agcgctggaa gccttgctgt
     4561 tctgacccct tgcctttttt cacaccgcgc cgcccgcgcc tgaccctggc ggcgccctga
     4621 cagttgatca cgctcatggc cctgctgcaa atcgccgaac ccggcctctt cgccgcccct
     4681 catcaacacc gcctggcggt tggcatcgac ctgggcacca ccaattcgct ggtggccacc
     4741 gtgcgcagcg gcgccgccgt ggtgctgccc gacgaacacg gccgccacct gctgccctcg
     4801 gtggtgcgct acaccccgga cggcgaatgc gtggtgggcc acgccgcgcg cgcgcggcaa
     4861 aacgaagacc cgcacaacac cattgtgtcc gccaagcgct ttatgggccg gggcctggcc
     4921 gatctggaca acccggcggc catgccctac cagtttgttg atgcgccggg catggtgcag
     4981 ctgctgaccc gcgccggggt gaaaagcccg gtggaaatct ccgccgacat cctgcgcgcg
     5041 ctcaaggccc gcgccgaagc cagcctgggc ggcgagctgg tcggcgcggt gatcaccgtg
     5101 ccggcctact ttgacgacgc ccagcgccag gccaccaagg acgccgcccg gctggccggg
     5161 ctgaacgtgc tgcgcctgct caacgaaccc accgccgccg ccatcgccta cggcctggat
     5221 aacgccagcg aaggcgtgta tgtggtgtac gacctgggcg gcggcacctt tgacgtctcc
     5281 atcctgcgcc tgtcgcgcgg ggtgtttgaa gtgctggcca ccagcggcga ttcggcgctg
     5341 ggcggcgatg actttgacca ccgcatttac tgttgggtgc tggaacaagc cggcctgtcg
     5401 caattgtcgg ccaacgacac ccgcctgctg ctcacccacg cccgcgccgc caaagaaatg
     5461 ctcaccgacg cacaagaagc gccaatccag gccacgctgt ccgatggcca actggtgcaa
     5521 ctgacgctga cgctggaaac cttcgagcag atcacccgca cgctggtgga caaaaccctg
     5581 ctgccggtgc gcaaggcgct gcgcgacgcc cggctgtcgg tggaggatat ccagggcgtg
     5641 gtgatggtcg gcggcgccac ccgcatgccg catatccgca aggcggtggc cgagcatttc
     5701 cgccagccgc cgctgaccaa cctcgacccc gaccaagtgg tggcgctggg cgccgccatg
     5761 caggccaatg tgctggccgg caataagggc gacgacgact ggctgctgct ggacgtgatt
     5821 ccgctgtcgc tgggcctgga aaccatgggc ggcctggtgg aaaaaatcat cccgcgcaac
     5881 agcaccatcc ccaccgcccg cgcacaagag ttcaccacct tcaaggacgg ccagaccgcc
     5941 atggccctgc atgtgctgca aggcgagcgc gagctggtgg ccgattgccg cagcctggcg
     6001 cgctttgagc tgcgcggcat tccgccgatg gtggcgggcg cggcgcgcat tcgcgtcacc
     6061 ttccaggtgg acgccgacgg gctgttgtcg gtcagcgccc gcgagcaaag cagcggcgtg
     6121 gaagcgcgca tcgaggtcaa gccgtcctac ggcctgaccg acgacgaagt cagccgcatg
     6181 ctgaccgaat ccatcgccca tgtgcacgac gacatcgccg cgcgcaagct ggcggaagcc
     6241 acggtggacg ccgaaggcct gctggccgcc atcgacgttg cgctggccag cgatggcgac
     6301 ctgctggacg ccgccgagcg cagcgcgctg gacgccgcca tcgccgccat gcgcgccgcg
     6361 ctggatgccc gccagacggt gggcatcaac caggccatgg ccacgctgaa cgaagccagc
     6421 ggcgactttg ccgcccgccg catggaccgc aatatccgtc gcgcgctgac cggccagaaa
     6481 atcagcgaac tgtaacctta cccccgccgc cggcgctgcg cccggcggcg tctgcgagac
     6541 acccaccatg ccgaaaatca tcgtcctgcc ccacgccgac ctgtgcccgc aaggcgcgct
     6601 gtttgacgcc gaaccgggcc tgagcatctg cgacaatctg ctgaacaatc aggtggacat
     6661 cgaacacgcc tgtgaaatgt cctgcgcctg caccacctgc catgtggtga tccgcgaagg
     6721 ctttgcctcg ctggagccgt ccgacgagct ggaagacgac ctgctggaca aagcctgggg
     6781 cctggaagcc acctcgcggc tgtcctgtca ggcgctggtg accgacaccg atctggtggt
     6841 ggaaatcccc agatacacca tcaaccacgc caaggagaac cattaatatg caatggaccg
     6901 acagcctgga catcgccatc gccctgtgcg acgcccaccc cgacatcgac ccgaaaaccg
     6961 tgcgcttcac cgacctgatg caatgggtgc tggcgctgcc ggaattcaac gacgatccgg
     7021 cccactgcgg cgaacgcatt ctggaggcga ttcagcaggc gtggattgac gaggtggaat
     7081 aagtccgtgc atcccctcct gcctgcatgg cagcgcaggg tgtgccgagt cagatcaagt
     7141 caatttctgc cgccagaaaa aaccaaagcc ccaccttgcg tggggctttg ctgtattggc
     7201 agtacatgcc agtgtggcta ctcactgctg gcatcctccc gccccagccg cacataaatc
     7261 ggctgatacg gcgcatcctg ggtgatgctg atcaggcctt ctttggccag ttccagaatg
     7321 gcgatgaaat tcaccaccac atagctgacg ttgatgtcca gctcgaagag ttcaacaaac
     7381 ggcacatggc cacggccttg cagctggcgc aggatgcggg tcatttgttc gcgcaccgac
     7441 aggctgtctt gctccacctg gtggtggcgg gtgtgtttgg cgcgggcgag gatggccatc
     7501 cacgcctggc gcaggtcggc cgggctgact tccggcaggc gcgcttcggc gctttgctcc
     7561 accagcaccg ccagccaggc aaagtcgcgc tcggcctgcg gcagcgcgtc cagtgccagc
     7621 gcggccagtt tgatttgctc gtattccagc aggcggcgca ccagctcggc gcgcggatcg
     7681 tccacttcgc cttcttcgtc gcgcggcggg cgcggcagca gcaggcggga tttgatttcg
     7741 atcagcagcg ccgccatcag caggtattca gcagccagct ccatgcgctc ggcgcgcatg
     7801 gcgccgatgt actccatgta ctgggcggtg acgcgcgcca tcgggatgtc cagcacattg
     7861 aggttttgct tgcggatcag gtagagcagt aaatccagcg ggccttcaaa gctttccaga
     7921 aacacccgca gcgcgtccgg cgggatgaac aaatccgccg gggcgtccag caccgcttcg
     7981 ccaaacacgg tggccagcgg cggcgccgat gcgggggcgt cggcggcaag cagggcgggc
     8041 gcgctcattg gcggcccgcc ggggtgatgc acgccggcat caggcgtagc ccagacccat
     8101 ggcttcgcgc acgtcgcgca tggtttcctg ggcaatggcg cgggcgcgct cgcagccgtc
     8161 ggcgacgatg ctcttcacca gggtggggtc gtccaggtat tgctgggcgc gttcgaacat
     8221 cggttgctgc tcggccagga tgccgtcgat caccggctgt ttgcattcca gacagccaat
     8281 gccggcgctg cgacagccgc tttgcaccca gtcgtgggtg tcgcggtcgg tgtacacctg
     8341 gtgcagctgc cacaccgggc atttgtccgg gctgcccggg tcggtgcggc gcacgcgcgc
     8401 cgggtcggtg ggcatgccgc gcactttttt gctgacagcg gccgggtctt cgcgcaggaa
     8461 gatgccgttg ccgtagctct tggacatctt ctggccgtcc aggccgggca tgcggctggc
     8521 ttcggtcagc agcgcctgcg gctcgaccag aatcaccttg cctttgtttt ccagatagcc
     8581 aaacagccgc tcgcggtcgc caatcgacag gttnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8701 nnnnnnnnnn nnnggcacgc gctccagcca ggacagcggg gtgatcatcg acagcaagag
     8761 gtgcagctcg gcgtgctcgg gcacccggct ctggatgaac agcgtggctt gtgatgggtc
     8821 cacgccggcg gccagccagt caatcaccat gtcccaggtg ctcttggcga tgatggacgg
     8881 gtcgtcgtag ctggtggtca gcgcgtgcca gtcggccaca aagaaaaagc actcatgttc
     8941 gctttgcagt ttcacccagt ttttcagcgc accatggtaa tgacccaggt gcaggctgcc
     9001 ggtcgggcgc atgccggaaa gaacacgttc ggaatacatg ggggagcttt cgcggttgaa
     9061 ttggcagggg gccgcgccgg cggccccgaa tgacgacaaa ttataacaag gcgttcagaa
     9121 aacgatagca ggccttgatg agcgggttca tcagcaagga cagcaggccg gtgaacatca
     9181 gcgcaatcag aatccacatg ccgtagggct ccagccgcga cagctgccag gcctggcggt
     9241 tgggcagcac gctcatcagc atgcgtccgc cgtccagcgg cggaatgggc agcaggttca
     9301 gcgccatcag catgacgttg atcagcatgc cggcctgcgc catcagcgcc agcggctcgg
     9361 tgtagtcgtt gttcatgccc agcgccagct tgaacatcag cgcccacagc accgccatca
     9421 gcaaattggc ccccggcccg gccgccgaca cccacagcat gtcgcgcttg gggtggcgca
     9481 gattgccaaa gcgcaccggc accggtttgg cccagccaaa gataaagccg cccaccgcca
     9541 cgcacaccag cggcagcagc accgtgccga tccagtcgat atggcgcagc gggttaagcg
     9601 tcatccgccc ttccagatag gcggtgttgt cgccaaagtg gcgggcggca taggcatgcg
     9661 ccgcctcgtg gccggtgatg gcaaaaatca ccggcagggc gaacaccgag attttttgaa
     9721 taaggttgag ttcgtccatg cgagtcccga ttcagaataa aaaccgctca cagaacttca
     9781 gtgcaagcag cacggcaagc aaccacggca ggagaatttt gtgagcggcg ttcagcgcgc
     9841 ccgcgtcggc gacgcgggct aggtgcttgg agccggcatc agcgcgcggg ttcaatgacg
     9901 ttcgagtttg actaggccag gcccagtttg tccagcggcc cggcgccggc gcggatcaat
     9961 tgcggcgcgc cgtcggtgag gtccagcacc gtggtgggct cgatgccgca atagccgccg
    10021 tcgatcacca agtccagctg gctttgcagc aggtcgcgga tttcaaatgg gtcgctgggc
    10081 ggcatcggtt cgtgcggcag catcagcgtg cacgacagca aaggctcgcc cagcgcctcc
    10141 agcagcgcca gcgccaccgg gtggtcgggc acgcgcaggc cgatggtgtt gcgcttgggg
    10201 tgcagggtgc ggcgcggcac ttccttggtg gcttgcagga taaaggtgta ggcgccgggg
    10261 gtgatggctt tcagcagccg gtattgggcg ttgtccaccc gggcatagct gcccagttcg
    10321 gacaggtcac ggcacaccag ggtgagatgg tggcgcaagt ccagttggcg gatggccagg
    10381 atgcgctcca tcgcccgctt gtcgcccaga tggcagccca gcgcgtaaca ggaatccgtc
    10441 gggtaggcga tgacgccgcc ttgctggatg attttcaccg cttcgcggat cagccgcgcc
    10501 tgcgggtttt cggggtggat gtgaaacagt tgggacatga tggtcttcag ttggccgcca
    10561 cgggcgtggc gcgcggcggg gtgaagcggc tccacaccgg gacgcacact ggcggcaaat
    10621 cctgggtgag ccccacgtcg cggccgcctt cgccaggggc atgaaagtcg ctgccggcgc
    10681 tggccagcag ctccatgcgc gcggccagca gggcaaattt atgggtttga tccagcgtgt
    10741 ggctggcgct gaccacttcg atggcctcgc cgcccaccgc tttgaaatcc tgcaccagct
    10801 cttcttgcaa ctggcggccg atgtcatagc gccccggatg ggcaatcacc gccacgccgc
    10861 cggcggcgcg aatccagccc accgcatcgg ccagttcggc ccactggtgc ggcacatagc
    10921 cgggcttgcc cttgaccaga tatttgcgaa acacggtggc gacgtttttc acctcgccgc
    10981 tttccaccag aaaacgggca aagtgggtgc ggccgatcat ttccgggttg ccgcacaagg
    11041 ccatcgcccc gtcaaacgcg ccatggatgc cgattttggc cagcgcggct tccatgcgcc
    11101 gcgcccgctc cacccgccct tcgcgcaccg cgcgcaggcc agcggccagc gccgggcaat
    11161 ccgggtcgat gtccaggccg acaatatgaa tggtgcgcgt gcgccagctg acagaaattt
    11221 ccacgccgcg aatcaggtcc agcccgtgct cgcgcgccgc ctcgccggcc tcggccaggc
    11281 cggcggtgca atcgtggtcg gtcagcgcca gcatgcgcga gccgcgcgcc gccgcacgcg
    11341 ccaccagctc ggcaggcggc aaagcgccat cagaagcggt ggagtggcaa tgcaaatcca
    11401 gtttggccat ggcgcatact cgggcaaagc aataagttta ccagcttgct tggcgcgctg
    11461 ccggggcgtt gcgctcggcc tcgctagagg ccgtttgccg ctgcgtccac tgccaatgct
    11521 tgagccggta atcagagagc tgagcctggt agacgtccag cacgcagttg tcgcaaccgc
    11581 tgccgcagca agcgccgtcg gcgggcggct cgggggcttc cggctctggg tcgaacacgt
    11641 cgtcaggctg gctcatgcgc ccaccacgcg gatggtgaag ccgtcgccgc tgacgatttg
    11701 gccggcgcga atcttgcagg ttttgcgcag ctcttgcgcg ccatcgacgc tgaccgcgcc
    11761 gctggccacc agcgccttgc cggcgccgcc gctgtcggcc acgccgcaga ttttcagcag
    11821 gtcgttgagg gcgatgtatt cgccagtcag ggtgaagtct tgttgcatgg ggaaaggtcc
    11881 ttcaaaaagc tgattatacg tgatggcggg gcatcagccc ggcttcaggt tggcttaagc
    11941 ttgcgcgcgc agcatcgcgg gacttttgcc attttggacc tgcgcccaca ccatgaagct
    12001 tttgaccctg ccctggcgcg ccccgcgcgc gaccttgctg cccgcggcat tttaccggcg
    12061 gcaatgggct ttactggccg tgctggcggc gctgttttat gccgcgctgg gcgatggccg
    12121 gctggatcac tggctggcgg cgttcagctt tgatccgctc agccagcact ttcccttaca
    12181 gcacaactgg tggctggagt atgtcaatca tgtctggctg aaagacgtgt ctattgtgat
    12241 tggtgtggcc gcgctggggc tgggggtgtt tggcccacgt tttggcctgg atgccgaggc
    12301 tgccaggcgc tggcggctgg cctgtgctgc gatggtggtc ggcagtctga gtgtatcttt
    12361 gctcaaacag gcttcaccgt atcactgccc atgggatttg ctggagtttg gcggccacgc
    12421 gccgtatgtg ccgctgctgg cgtccttgcc ggcaggggtg tcggcgggca agtgttttcc
    12481 ggccgggcat gcctcgggtg gctttgtctt gatggccttg tattttgttt atgcgggtga
    12541 gcgtccgcgt ctggcgcaag cctggctgtg gggcggactg gcgtttggct tgctgatggg
    12601 cgtgggtcag caattgcgcg gtgcgcactt tctgtcacat acgctgtggt cgggctgggt
    12661 ggtgtgggcc gctatgctgg cggtgaacca gttactgggc cggcgttgaa aaaaaatagg
    12721 tgaattcacc gattgactgt atcggatgga tgtcaagcat cggtaaaatc cgttaagctg
    12781 aaaatacatc cttttccgga gctgtcatga atcccggttc ttgcctggtc gatgatcctg
    12841 gcgttgccaa cctacagctg ctcagccatg ccgagcagca ggatatcctg ttgctgcaac
    12901 aggccattct cgaatcggtg gcgcaagggc acgacccgct ggacatcatc aacgaagtct
    12961 gctgcctgga agagcggctg ctgcccaatg ccgtgggctc ggtgatgttg ctggatgagg
    13021 atggcaagct gaacgtctac gctgcgccca gtgtgccgcc ggagggcgtg gagcgcctgc
    13081 gcctactgtg tccggggcca aattctggct cgtgtggcaa tgccttgtac cggggcgagc
    13141 ctgtgtttgt cagcgacaca ctgaccgacc cgcgctgggc cgatttgcgc gatctggcgg
    13201 tggatttcaa tctgctggcg tgttggtcga tgccgatata cggcgccggg cgcgagatta
    13261 tcggcacctt tgcgctgtcc agctttgagc gacgcgcgcc caccgagttt caccgcaagc
    13321 tgctggaaat cggcgcgtcg atcattggca tcgtgctgga gcgtcgccgc gaggcagaca
    13381 atctgcggct gatggccaag gtgttcgaga acagcaacga gggtattctg gtcaccgatg
    13441 cccaggcgcg catcgtgtcg gtcaacccgg catttgtcag catgacgggc tacagccgcg
    13501 acgaggcgct ggggcaaacc ccgcgcctgt tggcctctgg cattcatgat caggtttttt
    13561 atcagcacat gtgggccagc ctgcttgagc agggccactg gcatggcgaa atctggaacc
    13621 ggcgcaagaa tggcgaggtg tttccagaat ggctgagcct gtcggcggtg cacgaccaca
    13681 atggtgtgct gagtcattat gtcgggctgt tttttgatct gtccgagcgc aaggccgccg
    13741 aggcgcgcgc cgaatatttg attcaccacg atccactgac cgatctgccc aaccgcctgc
    13801 aagccaaaaa ccgcctgaac gaggcgattg cccgccgtca ggacgacggg gaaggcgtgg
    13861 ccctgctgta tgtggatctg gataatttca agaccatcaa tgattcactg ggccaggcaa
    13921 tgggcgatca attgctcaag gcggccgccc agcgcatgca aaccttgctg caaagccagg
    13981 aaatgctggc gcgtcacggc gcagatgagt ttttgctgat ggtgccgcat gtgcgcgaac
    14041 cgcgtcagct caccgagctg gccagcctgc tgctgaatgc gtttgccgcg ccgtttgaca
    14101 tcaacggtca gcaactgcac tccacgctgt cgattggcgt ggcgctggcg ccagacgatg
    14161 gcgacgatta cgacgcgctg ctgcaaaaag ccgaaactgc cgtgcgcgcc gccaaagaag
    14221 ccgggcgcaa caccttccgc ttttttaccg gccagcaggg cggccaggcc gccgagcaat
    14281 tgctgatccg caatggtctg cacaatgcgc tgttgcgcaa tgaattcatc ctgcactacc
    14341 aaccgcagct ggacctgacc accgggcgca ttgtcggcgc cgaggccttg gtgcgctggc
    14401 tgcaccccga acaaggcatg gtgccgccag gcaaattcat tccggtggca gaagaaagcg
    14461 gcctgattgt gccgattggc gaatgggtgc tgcacgaagc ctgccgccag ctggcgcaat
    14521 ggcgcgaaga ggggctgggc gatctggtgg tggcggtcaa cctctccggc gtggagttca
    14581 aacgcggcaa tctggtggaa agcgtcaccc acacgctggc gcgcaccggc ctgccggcca
    14641 cctgcctgga gctggagctc accgagtcca tgctgatgca cgatgtggaa aacgtgattt
    14701 ccaccttgca gcggctgaaa gcgctgggcg tgcgcctgtc gatcgacgac tttggcaccg
    14761 gctactcctg cctgtcctac ctcacccgct ttgacgtgga cacgctgaag atcgaccagt
    14821 cttttgtccg tcagctgcac ctgggcgacc agcacgccgc catcatccgc gcggtgatcc
    14881 agatggcgca cagcctggat ctggccacca tcgccgaagg cgtggaagac gccgaagtgg
    14941 tggctttcct gcgcgcacac gcctgcgacc aggcgcaggg ttatttcttc agcaagccct
    15001 tgccggcaga ggattttgcc cgctttgtcc gccagtatcg tgccgtgctg tagtcacgat
    15061 ggtgcatgcc tctggaatgc acccgacacg ccccgccccg tgcccttccg ctgaaaaatg
    15121 tcattggcgt ggctttggcc aattgccaaa gccaacgttt tcggtgacaa tgcctgactc
    15181 ctgtgcgggt gtagctcaat ggtagagcag aagcttccca agcttacgac gagggttcga
    15241 ttcccttcac ccgctccagc ccgccagtcg gcctgatttc tgccccaccc gtggcccttt
    15301 tgacccctgg cgcactgccg cctcacggag cttgattgcc ccatgaaatg gacatctggt
    15361 cttctccggc tgactcccta tcgcctcaaa atgctgggcg tactgcttgc cctgctggtg
    15421 ctggtgttca tcaccgccct gtcggttttt tacgcccgct tcgaccctga acaagtgcgc
    15481 atgcaattgg ccgatgcctt gcagactgat ggccgcactt tgagtatcgg gcagatttcg
    15541 cctgcggtgt ttccccgtcc cggcatcaag attgatcaaa tcagcctgag tgctcccaat
    15601 gccagcccgg atggcgacgg cccgctgttt accctgcgcc gcgccgaaat ccgcctggcc
    15661 tggctgccgc tgttgcaggc acaaaaagtg gtgcacgcca tcaagctgga cggtgtgcgc
    15721 ctgaacctgc gccgcgaggc ggatggacgc tggaattttg ccgacctgct ggagcagaaa
    15781 accgcgtctt ccctggtggt caagctcgat tcgctgaaac tggctgatct taacgccacg
    15841 gtggacgacc gggtgttcaa gcgcgaattc agcctgagcg aaggctcgat ggaggccagt
    15901 ggcatgctgg gcaatgccca gctgagcctg gccggtctgc tcaaaagcgg cgagcatcaa
    15961 gctgccgtgg tactggaaag cccgctgaac gttgccgaag aacaactgac gctggacccc
    16021 ttcaagctga cggccactgg cctggtggcc gggttgaccg acagccgtgc cgagctgacc
    16081 ggcaccgtgc gcgtcaacct ggtgtcgcgg cagatgaccg ccgaaaacct gcaagccagc
    16141 ctgagcagcc gcgagccgtc ggtttcgctg accgcgcgca tgcccagtgc cgactggtcg
    16201 cagggcaagg ccggttttca ggcgccctcg ctggatttca acgccgcgct gaacaacaag
    16261 ggctccagct atagtttttc tggtcagctc aaggatattt ccggcaactg gcaggcgctg
    16321 gccgccaccg aactgactgg cgcgatgagc tggaaagccg gcgaacacac cttgaccgcc
    16381 caactcaagg cgccagtgac gctgagcaat tgggaaatct tccgcctcac tccggtgacg
    16441 gtggacacca cgctggccac cccgctgctg ccgcgtggcc aactcaaggc tgccgccaag
    16501 ggtgagctgg tggccaatct ggcccgccag caagtcacca gcaagctcaa tggccagatc
    16561 gacggccagc gttttgactt caatgcccgc caggatggct ttatcacccc ggcgcacacc
    16621 gtcaatctca gcctgggcaa actcgatctt aaccgctacc tggccgaatc caccggcaat
    16681 caggtcggcc tgttttatgg ccagcgtaaa ttcgactttg actggatgaa cgcgctgaat
    16741 ctctctggag acttgcgcat tgccgaactc accgctggcc gttttgccat gcagcagatt
    16801 ggcgccaagc tgagcatgcg cccggaaaaa ctgctgctgg aagacgtcac cgcgctgatc
    16861 tacggcggca acctgaatgg ccgactggaa ctcacccccg gcgagcaacc caagctggtg
    16921 atgtttgaaa ccctcagcgg catgcgcatc cgcccgctgc tgcgcgacct gttcgacatc
    16981 gaccggctgg acggcaaagg ccggctggaa gccacgttga ccgccgaagg gcaaaccttc
    17041 acccagttgc gcaaatcgct gaacggcacc gtgcgcgtcg ggctgaccga aggcgcgctg
    17101 atgggggtgg acatggtgga agccatcaag cgcctgccca ccgagctggc gcggctgacc
    17161 gacgacccca gcaagcgcac cgattttcag agcctgtccg cccagttcaa cttcagcaac
    17221 ggcgtggcgc gcaatcacga cctgcggctg aactccagcc tgttcagcct gcaaggccac
    17281 ggcaaggtgg atttggtcgg caatatcatt gactacgcca tgcgcgtgca gcccaatatc
    17341 ctggtgttca ccaacaccaa agaaatgaac gtgccgctga aaatcaccgg cgcgctcaac
    17401 gcccccacct atgcgctgga tttcaatgcc ctggtgaagg acaaaaaaac catcctggaa
    17461 aaacagcagg tgctgaaaga acagctgagc aatcagttca atttgttcaa gaccacgccg
    17521 taatgccccc ccctgcgcca ccaggcctca atgccttgcc tggtggcatg caatctgctt
    17581 gtcatcgcct gccgttaagc ttaggctgtt gaccccatgc cctctttcat catgtcttta
    17641 agcgcttccc tgttgttcgc gctcaaaatc gccctggcca ctttgctggc ctggcctttg
    17701 ctcacctggc tgggcgttga gcagccggta tgggcggtgg tgaccattgc cgccgtcagc
    17761 gaccccaagc tcaccgcgac ggcggacttg gtgcgcgacc gggtgagcaa tacgctgatt
    17821 ggctgtgtgg tggccttgct gaccgtgtgg acggtgggcg cgcatctggg ctcgctggcg
    17881 gcggcgctgg cggtgtcggt ggtgctggcg cgcaccattg accattaccc cggcagctgg
    17941 cggcttaccc cgatcaccgt ggccattctg ctgggcagcg gcattggcct gccagcctcg
    18001 gcagcgctga atctggccgg gctgcgcgcg gcggaaatca tagtcggctc gctgctggcg
    18061 ctgggcttgg cctgggcgtt ttcctggtgg ccgcaccgtc gctggcgcaa ctggcgactg
    18121 gcgcggcagg gttgagcggc tgcctggcct gcgtgagcgg ctcaggctgg cttggcggcc
    18181 cgcgcgcgca gcatggtgac cgcttcgccg ccgatgcccc agttgtcggt gtccacttcg
    18241 tcaatcacca ccaccgtggt ggcggggttt ttgcccaggg tgtcgcgcag cagcgcggtg
    18301 acgccggcaa tcagcgcggc tttttgttcg gcgctggcgc cttcgcgggt gattttgatg
    18361 ttgacgtagg gcatggcggg ctccttttgg tgagtaaaac agcagtattc gtggccggcc
    18421 tgccgtggcg ggcgtcaggg cggcggcaac aggctgaata gttcggccgg gtgagtcagg
    18481 gtcagcgccg gctggcaggg gcgcaggccg tccaggctgg cgtagcccca gccgacggcg
    18541 ataaagtcca ccccggcgca ttgggcggcg tcgccgtcgg cgggttggtc gcccagataa
    18601 gcggcgtgtt cgggggcgat gtgcaggcgg cgcagcacct ggcgcagccg ctgcgccttg
    18661 cccagcagcg cgctgccgca ttccaccgcg gcaaagcgcg ccagccgcgc ctcgcccacg
    18721 ctgcgcgtca cattggcgcg gctgttcgag ctgaccagcg ccagctgctg gccggcgtcc
    18781 agctgggcat ccagccagtc cgccacgccg gcaaacaagg gcgcgctgtc cggcgcggcc
    18841 tgcatcaatt gcataaagtg ccgcgccacg cgcggcagct tccaccacgg caggccaaca
    18901 tggcgggcaa tctgtcgcgg gccgcagcgg cgcagcgccg gcagttcgtc ggccgccacc
    18961 gggcgaaagc catggtgggc ggccaactga ttgaaggtgt gccggaaaaa cggaaagctg
    19021 tcggccagca cgccgtcaaa atccagcatc agtaaacggt aggccatggt cagggggcgc
    19081 agcagaaaaa ccccgatatt acgccacccg acccggccgc gcggcatacc aggcgccggc
    19141 cgacaccgcg cacaagccca gcagcaggct ggcggtcagc ggctcgccca agagcggcac
    19201 ggcggccagc ccggccagca ccggcaccag gccaatcagc gcgccagtgc gcgccgcgcc
    19261 cagctgttgc accgccagca aaaacagcca catggcgatg atggtcgggc caatgccctg
    19321 atacaaagcc tgggtgatca gctgggtggc gctggcctcg gccaggcctt tgggcaacca
    19381 cagcgcgtac accggcaggt acagcagcgc cgagcccagc gcgacaaagc gggtgagcac
    19441 ccacggcgaa aacgcccatt gccgcaccag cacgctgtac accgcccagg tcagcgaggc
    19501 caccagcatc agcgcgtcgc ccggcagcgt gcgccagtcg ccggcttgca gcagcggcag
    19561 cgccacacac agcacgccca aggcaatgcc cgtcaggccg atcagccggc tgcgggtggg
    19621 gcgttcgccc agcgccagcc acagcagcgc cgtcaccaaa aacggctgca tgcccggcag
    19681 cagaatggcg ccgtgcgccg ccggggcgta tttgaaggcg gtgtacacca ccacgccata
    19741 cagcacgccg ccaatcgccg ccagcgtcca cagccggcgc tcgcgccaca ccgtgcgcgg
    19801 caagtcgcga cagaagggca atagcaagag cgaggccgcg cccaggcgca gcgccagtac
    19861 gtcatagccg gtcagcgggc ttttgccgcc ccagcgggaa atcagaataa agccagtcca
    19921 gatgcagacc acgcccaggg cggcggcgta gccctgccag ggcggacgag aagcagcatg
    19981 catggcgggc gctttcgggc ggtcagggag cttgactgta gcgcgtggcg aatcatcctg
    20041 taaaatgaat aatcctgatc aaacccatct caaaaacaga atatggacct ggacgacttg
    20101 cgtatttttc gggcggtggt gcgcgagggt ggcatcaccc gcgccgccgc ccggcttcat
    20161 cgggtgcaat ccaacgtcac cacccgcatc cgccagttgg aagcgcatct gggcgtgccg
    20221 ctgtttgtgc gcgaaggccg ccggctgagc ctggcgcccg ctggtgcggt gttgtctgat
    20281 tacgccgagc gcctgctgga gctggccgac caggcgcgcg ccgccgtggc cgacgccgcg
    20341 ccgcgcggcc gcctgcgtct aggttcgatg gaaagcactg cggctgcgcg gctgcccacc
    20401 cggctggcgg cgtttcatca gcgctacccg gacgtgcagc tggaactgca caccggcgcc
    20461 accgcgccgc tgctggcgca actgcgcgcg ggggagctgg attgcgtgct ggtgtgcggc
    20521 ccggtggccg acgcccggct gtgcgccgag ccggcgttta tggaaaccct ggcgctgatc
    20581 gccccggcgg gccatccgcc gattacccgc gcccgcgacg tgcgccccct caccctgctg
    20641 acctttgccc ccggctgcgc ctaccgccat cggctggagc aatggctggc cagcgacggc
    20701 gtgctgccgg agcgggtgat tgagctggcg tcctaccacg ccatgctcgg ctgcgtggcc
    20761 gccggcatgg gcattgcgct ggcgccgctg agcctgctgg acggcatggc ggccagccat
    20821 gcggtgtcgg tgcatccgct gccggccgaa cacgcgccag cccccaccct gctggtgcgc
    20881 cgggcgggag tggacagtcc gacggtgatg gcgctgcgtg aggtgatgtt ggcggggtga
    20941 atggcgaggg cgcaaaacgg tctgccaccg cgcgcatctg gccatgaaaa agcgtgtcgc
    21001 tgcatggcgc agcacggcac ggctgccccc gcgcagccag actctggcgc tagctgggct
    21061 ggaacacctc ggccagactg cgcgcatcca gcacgcggtc gctccaggct tccagttggg
    21121 ggagattggc gcgttgaata cgggtgaccg cgtcgacggg tagttcgcca aaacgacggg
    21181 tgagcaagcg cagcaataaa cgggcttcgc cttcctgttg cccctcctgc cggccttcct
    21241 gccggccttc ctgccgaccc ttttgcaggc cttcctgctt gccgcgttgc tcgtattgtt
    21301 gcgcccattg gtcaaagcgt tccgccaggg tcattttcag ctccttcaag tcacgcacct
    21361 tgggcaaggc cagggtgtgt ttgctttgcc gcaacag
//
LOCUS       DAOPYX010000010        21310 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_2531_length_21310_cov_11.151211, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000010 DAOPYX010000000
VERSION     DAOPYX010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 21310)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21310)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21310
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2531_length_21310_cov_11.151211"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..311
                     /locus_tag="PKN55_01145"
     CDS             <1..311
                     /locus_tag="PKN55_01145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX69879.1"
                     /translation="LDSALKYRRKNSVETFLQEATPLLTDAQKMCMLVNLIDSSLADG
                     QPEPEEQVLFGKFLTAFGISEDRFRPFFEVIVLKNDRSVFTNDHHPKNQPGYQVKLLG
                     "
     gene            596..1933
                     /locus_tag="PKN55_01150"
     CDS             596..1933
                     /locus_tag="PKN55_01150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011517535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric reductase-like transmembrane
                     domain-containing protein"
                     /protein_id="HNX69880.1"
                     /translation="MKTLHYSLWGGLLLLTGAWLFSESTPFAALNGFFAWRGVLNQYT
                     GALAMGVMSLAIILAVRPRWLEGPLDGLDKLYRLHKWLGIAGLTLAVTHWLLAKVPKW
                     LVGWGWLERPKRGPRPPLEPGSLEQIFAQQRGLAESVGEWAFYAAAVLMILALIKWFP
                     YRRFMQTHKWIAVAYLVLVFHSVVLVKFAYWSQPIGPLLAVLMALGSVAAVMSLFGVR
                     ASGRKVAGHVTAIQPYPALDALGVEIALAPGWPGHQAGQFAFVTLHGDEGAHPFTLAS
                     AWDPEQARVQVVIKSLGDYTGALAQRLRVGDAVQLEGPYGCFTFAGEARRQIWVAGGI
                     GVTPFMARLQARAEQGDDREVDFFYCTAQYEPMLIEPLQALAKQAGVRLHVWWNQRDG
                     RLDVAQLQARTPGWREADVWFCGPAGFGHSLREGLCGEGLPAARFHQELFEMR"
     gene            complement(1957..3297)
                     /locus_tag="PKN55_01155"
     CDS             complement(1957..3297)
                     /locus_tag="PKN55_01155"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNX69881.1"
                     /translation="MTARSHPPRVAVIGGGLAGLAAAWHLDAAGFACRVFESGKRPGG
                     PALSKREGACLFEYTPSVLQLDAALLAWLEQLGLGPALHPATPYASRRWVLHQGAYQP
                     WLESPLNWLLSRRVDRSSKRRRLVESLSLLRPGAVQPGGSVAAFVRAHLGDWFNDYLV
                     APWLALQYGVDAEQADMALTLPDLIQPASRLALARQLQARLLGVGGYGLRQGLDTLTD
                     TLSARVDVRLGQHVGALIRRPRGWCVVTGMETYEADLLVLAVSTREAALLLAPGFAQT
                     AARLGEVRYAPMIDVHSVYHRENMAFRLRAGGAWHPQCERPFALGVEFISSQISHRTP
                     DGEVLVCARLGGMAQAERLGLDDAVLGLLLDSELDGLLGVLNAPLERHVHRRRHAYPH
                     PDAALAAARAELPTLAQARVLVAADWAAGLGPEAVLRHAMTLPAAARAALAAPA"
     gene            3528..5171
                     /locus_tag="PKN55_01160"
     CDS             3528..5171
                     /locus_tag="PKN55_01160"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNX69882.1"
                     /translation="MSLLALLSLRTRLLLLFAVTLAVLLLTAAAGMLGVHRQSQLASE
                     LAQRDLGLSVALQAAHTRLSNMRRFEKDMLIQRREVAKVDDYFSKWQAEQQALQDSLA
                     RATALADGEQRTRLDAIGQQLTQGYAPVIAAVHQRLRMGEFDSAAAANEALNPGKVAA
                     HQAETLFSAAEHAVDTHMAGMAGQFASVRDSVMQGLLALLGGALLVLLVLFWLADRSI
                     RQPLQQLRADMRNIAQSLQLNQPVSAQGRDEVADAARAFNQLLDAMRGALLAVRNNAG
                     QLGDSARQLNQAADASLVSSRTQMGHATGVAEAVEQISQGMARISERAGAVQNLADQT
                     ARIAGQGVSMASRTAEGLGELQARLQESVVGVTRLNQRVADIGEVVIRIREVADQTNL
                     LALNAAIEAARAGEQGRGFAVVADEVRQLAENTAQATAAITRHIADIQGDTRQTVADI
                     HQLAGQVSAEMDEAHAFVSQLHTLREQAEHAHQQVASMAGDTAEQRQAGEHASAQVGH
                     LAGLSRAAGDTAASVAQLGDGLTQVAGQLDQAIGRFQLG"
     gene            complement(5357..5557)
                     /locus_tag="PKN55_01165"
     CDS             complement(5357..5557)
                     /locus_tag="PKN55_01165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008952480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HNX69883.1"
                     /translation="MSHPDTAPVVACPQCRRPVRWTTENRFRPFCSERCKLVDLGQWA
                     NEGYRIPAEHGPDGDAQRDERS"
     gene            complement(5554..6312)
                     /gene="zapD"
                     /locus_tag="PKN55_01170"
     CDS             complement(5554..6312)
                     /gene="zapD"
                     /locus_tag="PKN55_01170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011137371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HNX69884.1"
                     /translation="MISFEFPIVERVRILLRLEELFRRLTHFSDADGADEHHVALQTL
                     FDMAELGARGDLKSELIQDLERQRFALEALRDNPAIAEHALDEVLGEIESAMSRQLEY
                     TGKFGHELRDNEWLMNIKQRMAIAGGACQFDLPSYCYWQNLPAEARRADLQRWAAPLL
                     ATRDAVAVLLKLLRDSGKSNTFTARQGAFQQMSGGKVVHLIRVSIDRKLGVAPEVSAN
                     KYAVNVRFIEPTTHSARSRASTDDIPFTLAFCKL"
     gene            complement(6336..6971)
                     /gene="coaE"
                     /locus_tag="PKN55_01175"
     CDS             complement(6336..6971)
                     /gene="coaE"
                     /locus_tag="PKN55_01175"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011137372.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HNX69885.1"
                     /translation="MISTANGPRWVLALTGGIGSGKSAVAERLAALGAGVVDTDRVAH
                     ALTAPGGAAMPALAAAFGPEIVQADGAMDRAAMRARVFADPQARQQLEAILHPMIRVQ
                     TEAELATCPGDYLVLVVPLLVETGTYLPLADRVLVVDCPVETQRQRVHARNGLPEAQI
                     DAILASQASREARLTHADDIIDNSGDFAALGLQVDVKHSYYLALAHQALAD"
     gene            complement(6985..7761)
                     /locus_tag="PKN55_01180"
     CDS             complement(6985..7761)
                     /locus_tag="PKN55_01180"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF013632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HNX69886.1"
                     /translation="MSDLALPWPALLAMGLALASFVGVLAARWPAALADSPAPTAADL
                     LARLCRPPSHCPHCHTRLRIYELIPVLSWLAQRGRCRHCQAPLGMGDTLLELTGAALA
                     LLLGWRHGADGLGLAAGLLFAFTLLALAWLDARSLWLPDALTLPLLWAGLLYTSLRAP
                     AALPEHVWAAALGYGLLAGLNAIHLILRRRHGLGGGDAKLLAALGAWLGLWALPQILT
                     LAALAGLIWALFTRQRALAFGPWLAAAGLAGWLWPGFTLL"
     gene            complement(7758..9059)
                     /locus_tag="PKN55_01185"
     CDS             complement(7758..9059)
                     /locus_tag="PKN55_01185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012696147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HNX69887.1"
                     /translation="MLAYCACLDFRGRDALDDIPLSLLVAALVALLLISAFFSMSETG
                     MMAVNRYRLKNRAAQGHTGARLALRLLGQTDKLLGVILLGNNFVNSASASLATVITFR
                     LFGQNEAALGLATVAVTFAILIVSEATPKVIAATRPDAVANWASYPLTALLKLFYPVV
                     WFVNLFVKAAIRLLRVQPQPETTLLTPEELRVLVLESGQFIEKKHRSMLLNLFELENF
                     TVDDVMIPRRQLEMIDLDAPIEHIREQLMTSQHTRLPVYRGGYDNVVGFLHVRKVLHL
                     ATEDITVERLLSIVRPPYFVPAGTPLFTQLQNFQENQRRIAIVVDEYGEMMGLTTLED
                     ILEQVVGEFTTQAPGHSRLAQRQSDGSVLVDGGMNVRDLVKLLQVNFPLDGPKTVNGL
                     IVEYFQDIPEPGTCFRLSGCVFEVLQTQARGVKRVRITPPA"
     gene            complement(9215..10708)
                     /locus_tag="PKN55_01190"
     CDS             complement(9215..10708)
                     /locus_tag="PKN55_01190"
                     /inference="COORDINATES: protein motif:HMM:NF019351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase family protein"
                     /protein_id="HNX69888.1"
                     /translation="MHRRTVLTACLAAPFFPLLARAQGAVDSTMDHAAMGHAMPAAPA
                     AGASPRPPGGAPLAAWPTLGARGELRAAPGLLHWGGGLNTPSWGYNGAALGPLLEWRA
                     GEAVDMTLVNALPEASNIHWHGLEVPASEDGHPRDAVEAGQRRAYRFTVPEVDGLFWY
                     HPHPHGRTAFQVAMGLAGPIRVRGGRSPLPADGIDERVLMITDQRLLADGRIAPHSDA
                     DWMDGREGDLLLVNGQYQPVLSVRAGGWLRLRLINACAARYLHLSLPGHLWRQIGADG
                     GWLEQVQAPVKQLLLVPGERADVLVALDGAPGSRAVLQSLPYVRGKMVSAEQSRPEPI
                     LSVHYQRAAAQARPPLPRRLADIAPLPAPAVRREVVLSETMSMEGGKHQMAFLINDRR
                     YDDMNRADFTGKVGEVEEWTVHNRSDMDHPFHLHGAAFQVQWRQASAQHPRTPETLRA
                     WRDVVNLKPGESLALRFVQRLPGDRLFHCHILEHEDQGMMATLRVVG"
     gene            complement(10799..11104)
                     /locus_tag="PKN55_01195"
     CDS             complement(10799..11104)
                     /locus_tag="PKN55_01195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX69889.1"
                     /translation="MFNTFLRKHSEKVKIMLLPLLKNTRILLLAALLTPAMVMADGGL
                     DRVREMRERQASSAVNSPAAISSPQHSSMPQHGMPHCPSCPPQGMQPEHKMMMPGMK"
     gene            complement(11230..11976)
                     /gene="rlmB"
                     /locus_tag="PKN55_01200"
     CDS             complement(11230..11976)
                     /gene="rlmB"
                     /locus_tag="PKN55_01200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011134704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HNX69890.1"
                     /translation="MSNKKLIYGFHAVNARLFQNPKSLLELYIATGRQDARMRDAILK
                     AESEGIRILSVDSARLDGMSGHARHQGIAALIDTNKSFVTLDDVLENLTEPPLLLVLD
                     GVTDPHNLGACLRVADAMGAHAVIAPKDRSAGLNATVSKVACGAAEVVPYVTVTNLAR
                     TLRDLKDAGVWIAGTAMDASTDLFHCDAAGPLAWVMGSEGEGMRRLTRELCDVLVNIP
                     MFGTVESLNVSVASGMVLAESRRQRQVAAG"
     gene            complement(12188..12556)
                     /locus_tag="PKN55_01205"
     CDS             complement(12188..12556)
                     /locus_tag="PKN55_01205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX69891.1"
                     /translation="MKSSLIVLSLLSVSAAAWADGALKPMHGGVMAEAPSGLRIELAA
                     DPAGLAVYLRSHSDKLLASQGVSGDVVLLGAQGKQTAALTPAGDNKLTAALKPQPGAK
                     ALVRVTQPGLPVDQVRLSLP"
     gene            complement(12588..15692)
                     /locus_tag="PKN55_01210"
     CDS             complement(12588..15692)
                     /locus_tag="PKN55_01210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012614056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNX69892.1"
                     /translation="MLSWMVEHSLRNRLFVLVFALLMMAWGGMNAGRMAVDVFPDLNK
                     PMVTVMTEAGGMAPEDVELRVTQPLEAALNGIAGVQRVRSVSGAGLSVVYAEFAWGSD
                     IYRNRQMLAERLAEIREQLPPGVSPTLGPIASVMGEIMLIALPADPQQVSPMQVREFA
                     DFVLRPRLLSIPGVAQVIPIGGEVRQFRVEPDSAAMARLGVNLSQLEDALRGFAGNAS
                     GGFLAQHAREYLIRHIGQTLALSDLENLAVAERNGQPVLLRQLAQVKFAAAAKRGDAG
                     YNGKPAVVVSVQKQPQADSTRLTADIEAALASLSQRLPAGVQAPQVSFRQADFIQASV
                     ANVAEALRDGAIMVALILFLFLLNVRTTLISLTAIPLSLAVTLAVFHALDQSINVMTL
                     GGLAIAIGELVDDALVDVENVLRRLKALPAERSAADILRVIIAACNEVRSGVVVATLV
                     VVLVFVPLFALPGIEGRLFAPLGVAYITSILSSLLVALTVTPVLCYWLLPTLAARQHA
                     DSWLVRQLKRLVARALDWGFARWRALCLSAAVLGALVLLSAAWLPRAFLPAFNEGTLT
                     VSLLLNPGAALSESNRVGQLAEQLLLTVPEVKSVGRRTGRAELDEHAEGVHSSELDVD
                     LRPSARPREAVLLDIRQRLSSLPAAVAVGQPISHRLDHLLSGVRAQIAIKLYGDDLDT
                     LRGQAAQLRDRLAGVAGLADLQVEKQVLIPQIKVRVDYAQAAQYGVAPGALLARLEAL
                     TEGRVVSQIADGARRFQLVIRLPDAERDLTGLGRLLIDTPHGAIPLAQLTQLEESDGP
                     NQIGRENGRRRMVIAANTDGRDMAAVIADIRAALAATPLPAGYFVSLEGQFKAQEEAA
                     WRIAGLALISLTLIFMVLYSRYRSARLAALIMGSIPMAWVGSVAAMWLAGLTLSVASL
                     VGLITLTGIATRNGILKISHYLNLCKFEGESFGQAMIVRGTLERLTPVLMTALTAALA
                     LTPLLLAADAPGKEILHPVAVVIFGGLLSATVLDTLLTPLLFARLGRAPLEKLLAQAN
                     DTVF"
     gene            complement(15705..16847)
                     /locus_tag="PKN55_01215"
     CDS             complement(15705..16847)
                     /locus_tag="PKN55_01215"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNX69893.1"
                     /translation="MTRLNPRHTLLALALCLTAPWAHAHGDEVHDSPSPAPAAAPSAP
                     RNPAAASLALRPQRLPDGRVWLDKASQRQLGIRTQLSMLGEHRPSVELNGHVVMDPNA
                     GGRVQASLSGALEAGPDGLPSAGQRVRQGQLLAYLRPSLSAGERASQQAELAELHIKA
                     AQAARQLARLESLRETVAARDIDAQRAEAASLRQRAAALAAAGGRLALRAPASGVLSQ
                     ANALNGQQVEAGALLFEVVDPQKLRVEALAHDPAMAQQLGRASLPGSATPWQLVGSAT
                     AFRDGALPLLFAPQGRQDVPLALGQSVKLRVAYGAPVSGVALPAASVQRNSANQPVVW
                     LHDSAELFRPWPVQIAPLNGQDVLVRDLPAGRRVVSAGAALLNQIR"
     gene            complement(16844..17377)
                     /locus_tag="PKN55_01220"
     CDS             complement(16844..17377)
                     /locus_tag="PKN55_01220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX69894.1"
                     /translation="MIWRTLFVLTAWLLSAAAWAHGDESHDTAPPALGQAVTPRADAH
                     SEVFELLIVAEGEQLRLYLDDFASNAPISGARIEVERGTSWKAQAREHAPGEYLLKAP
                     WLNQPGAHPLSFTLEAGQQADLLAATLTLPATAAPAASAASLTPSMAGGAVAVIALTL
                     AAAWAWRRRHKATRTAP"
     gene            complement(17374..18567)
                     /locus_tag="PKN55_01225"
     CDS             complement(17374..18567)
                     /locus_tag="PKN55_01225"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNX69895.1"
                     /translation="MQASSFCRLALAVWLGLAAPAQAASLAEAFDRAWAARQAVSEAR
                     QQLRDSQREASDAWLPGPPALTLSQRSDRLDRNAGLREWEVELSAPLWLWGQRELARR
                     LAEREQDAEQHARALARWQLAGALREAWWEARLAELDAAAAQQRLHTQRALEADAMRR
                     VNAGQEAPLAHNQAAGARAQAETDWLRAQAQARRALNQFTAAARGAALPQQAEPAHPS
                     ALPDAHPLLASLDARVAAAQARLAQANGDSRDAPELALTLTRERGARSEPYQNLAKLS
                     LKIPFGGQARNGPRMAQANVELSEALAELHAGREQLAQDIDSARLARELGQQQARAQA
                     EALQLAEQRLGWIQRAVRAGQLSAPEQWRAQSELHEARSQAQRAQLEAGRAHSRYLQA
                     IGALP"
     assembly_gap    18744..18843
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            18863..19540
                     /locus_tag="PKN55_01230"
     CDS             18863..19540
                     /locus_tag="PKN55_01230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012202383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal response regulator transcription
                     factor"
                     /protein_id="HNX69896.1"
                     /translation="MRILIVEDEVRAAAYLRQGLSEAGFSVEVAHDGVEGEHLALTQA
                     FDLLILDVMLPGRSGWEILASVRRMSQTPVLFLTARDRVEDRVRGLEAGADDYLVKPF
                     AFSELLARVRTLLRRRPQAEPDVLRVADLELDLARHKAWRGGVRVTLTAKEMALLALF
                     MRRRGEVLSRTLIAEQVWDMHFDSDTNVVDVAVRRLRAKIDEPFELKLIHTLRGVGYV
                     LEAGRED"
     gene            19530..20930
                     /locus_tag="PKN55_01235"
     CDS             19530..20930
                     /locus_tag="PKN55_01235"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal sensor histidine kinase"
                     /protein_id="HNX69897.1"
                     /translation="MKTDWRRAWRGLTPRVVLGFAALTAALMVACGVYLYQALRLELG
                     ARDRDEQRGKIVLFRQALAGVSDAGMLRRNPDWLTHFMVGHDGLALRVLDARAAVLVD
                     SPAALPPGLWRGLDETPARLSGHDAHGHPWQGLAARATLADGQAVQLEIWRDGHDQEA
                     LLAQYRNHLLWAGALATLVAAALGGALVRHSLRPLAALTAAAMAIRPDTLNQRLDASL
                     APAELLGLVDAFNDALARLDAAFARLSDYAADLAHEMRTPLGILLGQTQVALSRGRSL
                     AEYQQVLADNAEELERLARTVNDLLFLAKTEHSEAALACEWLDVRQEVDEVCEFFALL
                     AEERDIQVQVEGALRLPVARAALRRLLNNLLSNAIRYSPPGSVVRVALDARLPGVAVN
                     NPPAAPLPEPLTMLFERFYSQGDGNGHGLGLPIARAIARQHGGELRASLGDDGRLTLA
                     AQLAAKASLVQARRCS"
     gene            complement(20909..>21310)
                     /locus_tag="PKN55_01240"
     CDS             complement(20909..>21310)
                     /locus_tag="PKN55_01240"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNX69898.1"
                     /translation="VRKLAERTTAATVDIAATVNEVRATTASAVSSMAGAAQAVEHSV
                     SALDASVAGLDAIERASEEVAGMAAHISAAAAQQGAASAEVAGNMQQVTQLVERNTEL
                     AHSATRAAQDVLAASHQLQALNRAFQLHRRA"
BASE COUNT         3491 a         6909 c         7271 g         3539 t
ORIGIN      
        1 tgctggattc cgcgctgaaa taccgccgca agaactcggt ggaaaccttc ctgcaagaag
       61 ccaccccgct gctgaccgac gcgcaaaaga tgtgcatgct ggtcaacctg attgattctt
      121 ccctggccga tggccagccg gagccagaag aacaggtgct gtttggcaaa ttcctgaccg
      181 cctttggcat ctcggaagat cgcttccgcc cgttctttga ggtgatcgtg ctgaagaacg
      241 accgcagcgt gttcaccaac gaccaccatc ccaagaacca gccgggctat caggtgaaac
      301 tgctgggcta atttcgcctg cttgctgcgc ggtgcgccac cacagcgcgc ctcgcttgtg
      361 cacatggctg acgcgcacat gccgtcggcc atgtgcattg gcgctgttgt gaacacagct
      421 ccccatcctt gccgcacgat gcgcacagcc tgcttgcgcc aggttgttga tcgcatcgag
      481 tttattgcgc caatttgtgg gtaagtaaca aaaagtaaaa ccccacttat ctatcttgct
      541 ggaaagataa tcgccgcgtt accctgaatg tcagatgaat ttgaggagtg acgcgatgaa
      601 aacgctgcat tacagccttt ggggcggttt gctgctgctc actggcgctt ggttgttcag
      661 tgaatcaaca ccgtttgccg cgctgaatgg tttttttgcc tggcgtgggg tactgaatca
      721 atacaccggc gcgctggcca tgggggtgat gagtctggcg atcattttgg cggtgcgccc
      781 gcgctggctg gaaggcccac tggacggatt ggacaagctg taccgcctgc acaaatggct
      841 gggcattgcc gggctgacgc tggcggtgac gcactggctg ctggccaagg tccccaagtg
      901 gctggtgggc tggggctggc tggaacggcc aaagcgtgga ccgcgtccgc cattagagcc
      961 tggctcgctg gagcagatct tcgcccagca acgtggcctg gcggaaagcg tgggcgaatg
     1021 ggcattttat gctgctgcgg tgttgatgat tcttgcgctg atcaagtggt ttccctatcg
     1081 gcgctttatg caaacgcata aatggattgc cgtggcctat ctggtgctgg tgtttcactc
     1141 ggtagtgctg gtgaagttcg cctactggag ccagccgatc gggccgttgt tggccgtgct
     1201 gatggcgctg ggcagtgtgg cggcggtgat gagtttattt ggcgtgcgtg ccagcgggcg
     1261 caaggtggct ggccacgtca ccgccattca accgtatccg gcgctggacg ccttgggcgt
     1321 ggagattgcg ctggcgcccg gctggcctgg gcatcaggcc gggcagtttg cctttgtgac
     1381 cttgcatggc gacgaaggcg cgcatccctt caccctggcc tctgcctggg atcctgagca
     1441 agcccgtgtg caagtcgtga tcaaatcgct gggggattac accggcgcgc tggcgcaacg
     1501 cctgcgggtg ggcgacgccg tgcaactgga aggcccgtat ggctgcttta cctttgccgg
     1561 agaagcacgg cggcaaatct gggtggctgg cggcattggc gtgacgccgt ttatggcgcg
     1621 tctgcaagca cgagctgaac agggcgacga ccgagaggtg gatttctttt attgcacggc
     1681 gcaatacgag ccgatgctga tcgagccgct gcaagcgctc gccaaacaag ctggtgtgcg
     1741 gctgcatgtg tggtggaatc aacgtgacgg ccggctggat gtggcgcagt tgcaggcgcg
     1801 cacgccgggc tggcgcgagg cggatgtgtg gttttgcggg ccggcgggct ttggtcactc
     1861 cctgcgcgag ggcttatgcg gcgaaggttt gccggcagcg cggtttcatc aggagctgtt
     1921 cgaaatgcgc tgatggtctg gcgtaacggc cggcgctcag gccggcgcgg ccagcgccgc
     1981 ccgggcggcg gcgggcaggg tcatggcgtg gcgcagcacc gcttctggcc ccagcccggc
     2041 cgcccagtct gccgccacca gcacgcgcgc ctgcgccagc gtgggtaact ccgcgcgggc
     2101 ggcggccagc gcggcgtccg ggtgcgggta ggcgtggcgg cggcggtgca catggcgttc
     2161 caatggggcg ttgagtacgc cgagcaggcc gtccagttcg ctgtccagca acaggcccaa
     2221 caccgcgtcg tccagcccca gccgctcggc ctgggccatg ccgcccaggc gggcgcacac
     2281 cagcacttcg ccatccggcg tgcggtggct gatctggcta ctgatgaatt ccacgcccag
     2341 ggcaaacggc cgctcgcact gcggatgcca ggcgccgccg gcgcgcagcc gaaacgccat
     2401 gttttcgcgg tgatagacgc tgtgcacgtc gatcatcggc gcgtagcgca cctcacccag
     2461 ccgcgcggcg gtctgggcaa agccgggggc cagtaacagt gcagcttcgc gggtggacac
     2521 cgccagcacc agcaagtcgg cttcataggt ttccatgccg gtcaccacgc accagccgcg
     2581 cgggcgacga atcagcgcgc cgacatgctg gcccagccgc acgtccaccc gcgccgacag
     2641 agtgtcggtc aaggtgtcca gcccctggcg caaaccgtag ccgcccacgc ccagcaggcg
     2701 cgcctgcaac tggcgcgcca gcgccagccg gctggctggc tgaatcaggt cgggcagcgt
     2761 cagcgccatg tcggcttgtt ctgcatccac gccgtattgc aaagccagcc aaggcgccac
     2821 cagatagtca ttgaaccagt cgcccaaatg cgcgcgcaca aacgccgcca cgctgccgcc
     2881 cggctgcacg gcgccggggc gcaacaggct gagcgactcc accagccgac ggcgtttgct
     2941 gctgcgatcc actcgccggc tgagcagcca gttcagcggt gactccagcc agggttgata
     3001 cgctccctga tgcagcaccc agcgccggct ggcgtagggc gtggccgggt gcagcgccgg
     3061 gcccaggccc aactgctcca gccaggccag cagcgcggcg tccagttgca gcacgctggg
     3121 ggtgtattca aacaggcaag cgccttcgcg tttggacaag gccgggccgc cggggcgttt
     3181 gccagactcg aacacccgac aggcgaagcc ggcggcgtcc agatgccagg cggcggcaag
     3241 gccggccagg ccgccgccaa tgacggccac gcggggcgga tgagatcggg ctgtcatggc
     3301 gagaaacgtc tcctcagggt tcgtgcatcg gggtatttga tcaattattg caatgcagca
     3361 tgcgcatggc aagccagtga cgacctcctg gcctggcaaa tgtccgtttg atgaagtttc
     3421 atgaaaacgc aattttcttg cgatgtgaat cccctatgat gtacggcggc cacgccgtgg
     3481 aaatttcctt atggcgggcg ttttgtccat tatctcaagg acctgtcatg tcgctgcttg
     3541 ctctgttgtc cctgcgcacc cgcttgttgc tgctgtttgc cgtcaccctg gccgtgttgc
     3601 tgctgaccgc cgccgccggc atgctgggcg tgcaccgtca gtcgcagctg gccagcgagc
     3661 tggcgcaacg cgatctgggc ctgagtgtgg ccttgcaggc ggcgcacacc cgcttgtcca
     3721 atatgcgccg ttttgaaaag gacatgctga ttcaacgccg cgaagtggcc aaggttgacg
     3781 actacttcag caaatggcaa gccgagcagc aagccttgca agattcgctg gcgcgcgcca
     3841 cggcactggc cgatggcgag cagcgtaccc ggctggacgc cattggccag caactgaccc
     3901 agggatatgc gccggtgatt gccgccgtgc accagcgctt gcgcatgggc gaatttgatt
     3961 ctgccgccgc tgccaacgag gcgctgaacc ctggcaaagt tgcggcgcac caggccgaga
     4021 cgctgttcag cgctgccgag cacgctgtcg atacccacat ggccggcatg gctggccagt
     4081 ttgccagtgt gcgcgacagc gtgatgcagg gcttgttggc tttgctgggt ggcgcgctgc
     4141 tggtgttgct ggtgctgttc tggctggcgg atcgctcgat tcgtcagccc ttgcagcagt
     4201 tgcgcgccga tatgcgcaac atcgcccaat ccctgcaact gaaccagccg gtcagcgcac
     4261 agggtcgcga cgaagtcgcc gatgccgcgc gggcattcaa ccagttactg gacgccatgc
     4321 gcggcgcctt gctggccgtg cgcaataacg caggtcaatt gggtgacagc gcccgtcagc
     4381 tgaatcaggc ggctgatgca tcgttggtca gcagtcgcac ccagatgggc cacgccactg
     4441 gcgtggccga ggcggtggag caaatcagcc agggcatggc gcggatttcc gagcgggccg
     4501 gtgcggtgca aaacctggcc gaccagactg cgcgcattgc tggccaaggg gtcagcatgg
     4561 ccagccgcac cgcagagggt ctgggcgagt tgcaagcgcg cttgcaagag agcgtggtag
     4621 gggttacccg gctgaatcag cgggtggcgg acattggcga ggtggtcatc cgtatccgtg
     4681 aagtggccga tcaaaccaat ctcttggcgc tgaacgccgc cattgaagcc gcccgcgccg
     4741 gcgagcaggg acgcggtttt gccgtggtgg ccgacgaagt gcgccaattg gcggaaaaca
     4801 ccgcacaagc cacggcggcc atcacccgtc atattgccga catccagggc gacacccggc
     4861 agaccgtggc cgatattcat caactggccg gtcaggtcag tgccgaaatg gacgaagcgc
     4921 acgcctttgt cagccaactg cacaccctgc gcgagcaggc cgagcatgct caccaacagg
     4981 tggccagcat ggcgggagac accgccgagc aacgccaggc cggcgagcat gccagtgccc
     5041 aggttggcca tctggctgga ttgtcgcggg cggcgggtga caccgccgcc tcggtggcac
     5101 aactggggga tgggctgacg caggttgccg ggcagctgga tcaggccatt ggccggtttc
     5161 agctgggctg agtgtggcag gtatgcggcc aggtgatggg ggccagactt gaggagcagg
     5221 gcgcgcagcg gggttggttg gaagccgctg accaacgtca gcgcagcggc tatggccagg
     5281 cgcgcggctg cagacagtac aagccgtacg gtataaggcc gtgcaatgat gccaggtgtg
     5341 tttttgttcg ccgcgcttag ctgcgctcgt cgcgctgggc gtcgccatcc gggccatgtt
     5401 ccgccggaat gcggtagccc tcattcgccc actgaccgag gtcaaccagc ttgcaacgct
     5461 cgctacaaaa tggccggaag cggttttctg tggtccagcg caccgggcgg cggcactgcg
     5521 ggcaggccac cacgggggcg gtatcgggat ggctcatagc ttgcaaaagg ccagggtgaa
     5581 ggggatgtcg tcggtcgagg cgcgcgagcg ggcgctgtgc gtggtgggct cgataaagcg
     5641 cacattgacc gcgtatttat tggccgacac ctcaggcgcc acgcccagct tgcgatcaat
     5701 gctgacgcgg atcaagtgca ccaccttgcc gccggacatt tgctggaacg caccctggcg
     5761 ggcagtgaag gtattgctct tgccgctatc gcgcagcagt ttcaacagca ctgccaccgc
     5821 atcgcgggtg gccagcagcg gcgcggccca gcgttgcaga tcggcgcgtc gcgcctccgc
     5881 cggcagattt tgccaatagc aataagaagg cagatcaaac tggcaggcgc cgccggcaat
     5941 ggccatgcgc tgcttgatat tcatcagcca ttcattgtca cgcagctcat ggccaaattt
     6001 gccggtgtat tccagctggc ggctcatggc cgattcgatc tcacccaata cttcgtccag
     6061 cgcgtgttcg gcgatggcgg gattgtcgcg cagcgcctcc agcgcaaagc gctggcgctc
     6121 cagatcctgg atcaactcgg atttgaggtc gccgcgcgcg cccagctcgg ccatgtcgaa
     6181 cagcgtttgc agggcgacat ggtgctcgtc ggcgccgtcg gcgtcgctga agtgggtcag
     6241 ccgacgaaac agctcctcca gccgcagcag gatgcgcaca cgttcgacaa tgggaaattc
     6301 aaagctgatc acaaggccac tgcaacaggt tggagttagt cggcaagcgc ctgatgcgcc
     6361 aaggcgagat aatagctgtg tttgacgtcc acttgaagcc caagggcggc aaaatccccg
     6421 gaattgtcaa taatatcgtc agcatgtgtc agcctggcct cacggctggc ctgactggcc
     6481 agaatggcgt cgatttgcgc ctctggcagg ccattgcgtg cgtgcacccg ctggcgctgg
     6541 gtctctaccg ggcagtccac caccagcacc cggtcagcca gcggcagata ggttccggtt
     6601 tccaccaaga gcggcaccac cagcaccaga taatcccccg gacatgtcgc cagctcggcc
     6661 tcggtctgca cgcggatcat cgggtgcaaa atagcctcca gttgctggcg cgcctggggg
     6721 tcggcaaaca cccgcgcgcg catggcggcg cggtccatgg cgccgtcggc ctgaacaatc
     6781 tcaggcccaa acgccgccgc cagcgccggc atggcagccc cacccggcgc agtcagcgcg
     6841 tgcgccaccc ggtcggtgtc caccacgccg gcgcccagcg ccgccagccg ctcagccact
     6901 gcgcttttgc cgctgccaat gccgccggtc agcgccagca cccagcgtgg gccgtttgcc
     6961 gtactgatca tgataacgct cctgtcataa tagtgtgaac ccaggccaca accagcccgc
     7021 cagcccagct gccgccaacc atggcccaaa cgccagcgcg cgctggcggg tgaatagcgc
     7081 ccagatcagc ccggccagcg ccgccagggt gaggatctgt ggcagcgccc acagccccag
     7141 ccaggcgccc agcgccgcca gcagtttggc gtcgccgccg cccaggccgt ggcggcggcg
     7201 caagatcagg tggatggcat tcagcccggc cagcaggcca tagcccagcg ccgccgccca
     7261 gacatgctcg ggcagcgcgg ccggggcgcg caggctggtg tacagcaggc cggcccacag
     7321 caggggcagg gtcagcgcgt cgggcagcca caggctgcgc gcatccagcc aggccagcgc
     7381 cagcagggtg aaggcaaaca gcaggccggc ggccagacca aggccatcag cgccgtggcg
     7441 ccagcccagc agcagcgcca aggcggcgcc ggtcagctca agcagggtgt cgcccatgcc
     7501 cagcggcgcc tggcagtgtc ggcagcggcc acgttgcgcc agccagctca acaccgggat
     7561 cagttcgtat atgcgcagcc gagtgtggca atgcgggcag tgcgacggcg gccgacacag
     7621 ccgcgccagc aaatccgccg ccgtgggggc cgggctgtcg gccagcgccg ccggccagcg
     7681 cgccgccagc acgccaacaa agctggccag cgccaggccc atcgccagca gcgccggcca
     7741 cggcagcgcc aggtcgctca tgccggcggg gtgatgcgca cgcgcttgac gccgcgcgcc
     7801 tgggtttgca gcacctcaaa cacgcagccg gacagacgaa agcaggtgcc cggctcgggg
     7861 atgtcctgaa aatattccac aatcaggccg ttgacggttt tcgggccgtc cagcggaaag
     7921 ttcacctgca acagcttgac cagatcgcgc acgttcatgc cgccatccac cagcacgctg
     7981 ccatcgcttt gccgctgcgc cagtcggcta tgccccggcg cttgcgtggt gaattcgccc
     8041 accacttgct ccaggatgtc ttccagcgtg gtcagcccca tcatctcgcc gtattcatcc
     8101 accacgatgg cgatgcggcg ctgattctct tgaaaattct gcaattgggt gaacagcggc
     8161 gtgccggccg gcacaaagta aggcggccgc acaatgctga gcagccgctc gacggtgatg
     8221 tcttcggtgg ccaggtgcag caccttgcgt acatgcagaa agcccaccac attgtcgtag
     8281 ccgccgcgat acaccggcag gcgagtgtgc tggctggtca tcagctgttc gcggatatgc
     8341 tcgatgggcg cgtccaggtc gatcatttcc agctggcggc gcggaatcat cacatcgtct
     8401 acggtgaagt tttccagctc gaacaggttc agcagcatgc tgcggtgttt tttctcgatg
     8461 aactggccag actccagcac cagcacgcgc aattcttccg gggtcagcag ggtggtttcc
     8521 ggctgcggct gcacgcgcag caggcggatg gcggctttga caaacagatt gacgaaccac
     8581 accaccgggt aaaacagctt gagcagcgcg gtcagcgggt agctggccca gttggccacc
     8641 gcatccgggc gggtggcggc aatcaccttg ggcgtggctt ctgacacgat caggatggca
     8701 aacgtcaccg cgacggtggc caggcccagc gcggcctcgt tctgaccgaa caggcgaaac
     8761 gtgatgacgg tggccagcga ggccgacgcc gaattgacaa aattattgcc cagcagaatc
     8821 acgcccagca gcttgtcggt ttgtcccaac agccgcagcg ccagccgcgc gccggtgtgg
     8881 ccttgcgccg cccggttttt cagccggtag cggttgaccg ccatcatgcc ggtttccgac
     8941 atggaaaaaa acgccgagat cagcaacagc gccaccagcg ccgccaccag cagggataag
     9001 ggaatgtcgt ccaaggcgtc gcgcccccga aaatcaagac acgcgcagta tgccagcatg
     9061 gcggcggttt gtgcggatgg gatcagaacg caatatgcat cctgatccca tcgttctttt
     9121 tcctgaatgt ggtgtgaccc cgcttggcct ggttttcgcc agcgcggcag ccggcacgga
     9181 gcgcggtggc ccagcacacg gcggtgagtg cggcttaccc caccacccgc agcgtagcca
     9241 tcatcccctg gtcttcgtgt tccagaatat ggcagtggaa cagccgatca ccgggcaggc
     9301 gttgcacaaa gcgcagcgcc agcgattcgc ccggcttcag gttgaccacg tcgcgccagg
     9361 cgcgcagggt ttccggcgtg cgtgggtgtt gggcgctggc ttgccgccat tgcacctgaa
     9421 acgctgcacc gtgcaggtgg aaggggtggt ccatgtctga gcggttgtgc accgtccatt
     9481 cttccacttc gcccaccttg ccggtgaaat cggcgcggtt catgtcgtcg tagcggcggt
     9541 cgttgatcaa aaaggccatc tggtgtttgc cgccttccat cgacatggtt tcgctcagca
     9601 ccacctcgcg gcgcacggcc ggcgccggca gcggggcgat gtcggccagg cggcgcggca
     9661 gcggcgggcg cgcctgggcg gcggcgcgct ggtaatgcac gctcaggatg ggctcgggcc
     9721 ggctttgctc ggcactgacc atcttgccac gcacataggg cagcgattgc aacaccgccc
     9781 ggctgcccgg cgcgccatcc agcgccacca gcacatcggc gcgctcgcct ggcaccagca
     9841 agagttgttt gaccggcgct tgcacctgct ccagccagcc gccatcggcg ccgatttgcc
     9901 gccagagatg gccgggcagg ctcaggtgca ggtagcgggc ggcgcaggcg ttgatcaggc
     9961 gcaggcgcag ccagccgcca gcgcgcacgc tcagcaccgg ctggtattgg ccgttgacca
    10021 gcagcaggtc gccttcgcgg ccgtccatcc agtcggcgtc gctgtgcggg gcaatgcgtc
    10081 cgtcggccag caggcgctgg tcggtgatca tcagcacgcg ctcgtcgatg ccgtcggcag
    10141 gcagcggcga gcgcccgccg cgcacgcgaa tcggcccggc caggcccatg gccacctgaa
    10201 aggcagtgcg gccgtgcggg tgtgggtgat accagaacag gccgtccacc tcgggcacgg
    10261 taaagcgata ggcgcggcgc tggccggcct ccaccgcgtc gcgcgggtgg ccgtcttcgc
    10321 tggctggcac ttccagccca tgccagtgga tattgctggc ttcgggcagg gcgttgacca
    10381 gggtcatgtc caccgcctcg cccgcgcgcc attccagcag cgggcccagc gccgcgccgt
    10441 tatagcccca gctgggggtg ttcagcccgc cgccccagtg cagcaggccg ggcgcagcgc
    10501 gcaattcgcc gcgcgcgccc agcgtgggcc aggcggccag cggcgcgccg cccggcggac
    10561 gcggcgacgc gccggcagcc ggcgccgccg gcatggcgtg gcccatggcg gcgtggtcca
    10621 tcgtggaatc gaccgcgcct tgggcgcggg ctaatagtgg gaaaaacggg gccgccaggc
    10681 aggcggtcaa tacggtgcgg cgatgcatca gacgtgctcc gatcagaaag cggttggcga
    10741 ccaaaagccg caacgccaca ttgcctaaaa atcagcctgt ggcgcgcaca tgggaggctt
    10801 atttcatgcc cggcatcatc atcttgtgct caggctgcat gccttgcggc gggcaggacg
    10861 ggcagtgtgg catcccgtgt tgtggcatgg atgagtgctg aggggaggaa atcgcggctg
    10921 ggctgttgac cgcgctggat gcttggcgtt cccgcatctc gcgcacgcgg tccaggccgc
    10981 catccgccat gaccatggcc ggcgtcagca aggcggccag caacaggatt cgggtgtttt
    11041 tcaggagggg cagcagcatg atcttgacct tttcagaatg tttccgcaga aacgtgttga
    11101 acatggtgag atgatgcctg ccgcccctgg tcacagagct gacccgcgca tgacaattct
    11161 gtcatgcatg gtgggggcaa ggcgcgcgcc gcaaacaggc atggcggcgc ggggttggcg
    11221 gggtggggtt tagcccgccg ccacctggcg ctggcggcgc gattcggcca gcaccatgcc
    11281 ggaggccacc gacacattca ggctttccac cgtgccgaac atcgggatat tcaccagcac
    11341 atcgcacagc tcgcgggtga gccggcgcat gccttcgcct tccgagccca tcacccaggc
    11401 cagcgggccg gcggcgtcgc agtggaacaa atcggtgctg gcgtccatcg ccgtgccggc
    11461 aatccacacg ccggcgtctt tcaggtcacg cagggtgcgc gccagattgg tgacggtgac
    11521 gtagggcacc acttccgccg cgccgcaggc caccttgctc accgtggcgt tcagcccggc
    11581 cgagcggtct ttcggggcaa tcaccgcgtg cgcgcccatg gcgtcggcca cgcgcaggca
    11641 ggcgcccaga ttgtgcgggt cggtcacccc atccagcacc aggagcagcg gcggttcggt
    11701 gaggttttcc agcacgtcgt ccagggtgac aaagctcttg ttggtgtcga tcagcgcggc
    11761 aatgccctga tggcgggcgt ggccgctcat gccgtccagg cgggcgctgt ccacgctcag
    11821 gatgcggatg ccttcgctct cggctttcag gatggcgtcg cgcatgcgcg cgtcctggcg
    11881 gccggtggcg atgtacagct caagcaggct tttcgggttt tgaaacaggc gggcgttaac
    11941 ggcgtggaag ccgtaaatca gttttttgtt ggacacggcc aggctttcat ttgcaaatta
    12001 agcgctattt taagccagcc aaggcgtggc gcacggggcg cgcgcaggaa tattttgcga
    12061 gcaggcgccg ccggcttggc ggcgcctgct cggggcgctc acaaaacccc gtgaagcgac
    12121 cctggctggg cgcgcgcaga cagtgcaagc agtacggcaa ggccgggcaa ccacgccagg
    12181 aggactctca aggcaggctg agccgcacct gatccaccgg caagcccggc tgggtgacgc
    12241 gcaccaaggc tttggcgcca ggctgcggct tcagcgcggc ggtgagttta ttgtcgccgg
    12301 ccggggtcag cgcagcggtt tgcttgcctt gtgcgcccag cagcaccaca tcgccgctga
    12361 cgccctggct ggccagcagc ttgtccgagt gcgagcgcag atacaccgcc aggccggccg
    12421 ggtcggcggc cagttcaatg cgcaggccag acggcgcttc cgccatcacc ccgccgtgca
    12481 tgggcttgag cgcgccatcc gcccaggcgg cggcgctgac agacaacagg gacaacacaa
    12541 tcaaactgga tttcatggca aaggcctttg tcaaaaagag aaacagatca aaatacggtg
    12601 tcgttggcct gcgccagcag tttttccagc ggcgcgcggc ccagtcgggc aaacagcaag
    12661 ggggtgagca gggtgtccag cacggtggcg ctgagcaggc cgccaaaaat caccaccgcc
    12721 accgggtgca ggatttcctt gccgggcgca tcggcggcca gcagcagcgg agtcagcgcc
    12781 agcgcggcgg tcagcgcggt catcagcacc ggcgtcagcc gctccagcgt gccgcgcaca
    12841 atcatcgcct ggccaaagct ttcgccctca aatttgcaca ggttcaaata gtggctgatt
    12901 ttcaggatgc cattgcgggt ggcgatgccg gtcagggtga tcagccccac cagcgaggcc
    12961 accgacagcg tcagcccggc cagccacatc gccgccacgc tgcccaccca ggccatgggg
    13021 atgctgccca tgatcagcgc cgccagccgt gctgagcggt aacggctgta cagcaccata
    13081 aaaatcagcg tcagcgagat cagcgccagc ccggcaatgc gccaggcggc ttcttcctgc
    13141 gccttgaact ggccttccag gctgacaaaa tacccggccg gcagcggcgt ggcggccagc
    13201 gcggcgcgga tgtcggcaat caccgccgcc atatcgcggc catcggtgtt ggcggcaatc
    13261 accatgcgtc ggcggccgtt ttcccggcca atctggttgg ggccgtcgct ttcttcaagc
    13321 tgcgtcagtt gcgccagcgg gatggcgcca tgcggggtgt cgatcagcag gcggcccagc
    13381 ccggtcaggt cgcgctcggc gtcgggcagg cggatcacca gctgaaagcg gcgcgcgccg
    13441 tcggcaatct ggctgaccac ccggccttcg gtcagcgctt ccagccgggc cagcagcgcg
    13501 ccgggcgcca cgccgtattg cgcggcctgg gcgtagtcca cccgcacctt gatttgcgga
    13561 atcaacacct gtttttccac ttgcagatcg gccagcccgg ccacgccagc cagtctatcc
    13621 ctcagttgcg ccgcctggcc gcgcagggtg tccaggtcgt cgccgtacag cttgatggcg
    13681 atttgcgcgc gcacgccaga cagtagatgg tcaagccggt gcgaaatcgg ctggcccacc
    13741 gccaccgccg ccggcaggct ggacaggcgc tggcgaatgt ccagcaatac cgcctcacgc
    13801 ggccgcgccg acgggcgcag gtccacatcc agctcgctgg agtgcacgcc ctcggcgtgt
    13861 tcgtccaact cagcgcggcc ggtgcgccga cccacgcttt tgacttcggg cacggtcagc
    13921 agcaattgct cggccagttg ccccacccgg ttggattcgc tgagcgcggc gccggggttg
    13981 agcaacagcg acacggtcag cgtgccctcg ttaaatgccg gcaaaaacgc gcgcggcagc
    14041 caggcggcgc tgagcagcac cagcgcgccc agcacggcgg cgctcaggca cagcgcgcgc
    14101 cagcgggcaa agccccaatc cagcgcgcgc gccaccaggc gcttgagctg gcgcaccagc
    14161 cagctgtcag cgtgctggcg cgccgccagg gtgggcagca gccagtagca cagcactggc
    14221 gtcaccgtca gcgccaccaa caggctggac agaatcgagg tgatgtacgc cacgcccagc
    14281 ggggcgaaca gccggccttc gatgccgggc agggcaaaca gcggcacaaa caccagcacc
    14341 accaccaggg tggccaccac cacgccggag cgcacctcgt tgcaggcggc gataatcacc
    14401 cgcaggatgt ccgctgccga gcgttcggcc ggcagcgcct tcagccggcg cagcacgttt
    14461 tccacgtcca ccagcgcgtc gtccaccagc tcgccaatgg cgatggccag cccgcccagg
    14521 gtcatgacat tgatcgactg gtccaaagcg tgaaacaccg ccagggtgac ggccagcgac
    14581 agcgggatgg cggtcagcga gatcagcgtg gtgcgcacat tcagcaaaaa caaaaacaga
    14641 atcagcgcca ccatgatggc gccatcgcgc agcgcttcgg ccacattggc caccgaggcc
    14701 tgaataaagt ccgcctggcg gaacgacacc tgcggcgctt gcaccccggc cggcaggcgt
    14761 tggctcaggc tggccagcgc ggcttcgatg tcggcggtga gccgggtgga atctgcctgc
    14821 ggttgttttt gcacgctgac caccacggcg ggcttgccgt tgtagccggc gtcgccgcgc
    14881 ttggcggcgg cggcaaattt gacttgcgcc agctgacgca gcagcaccgg ctggccattg
    14941 cgctcggcca ccgccaggtt ttccaggtcg gacagcgcca gggtctggcc aatatggcga
    15001 atcaggtatt cgcgggcgtg ctgcgctaaa aaaccgccgc tggcgttgcc ggcaaagccg
    15061 cgcaaggcgt cttccagctg gctgaggttg acgcccagcc gcgccatggc ggcgctgtct
    15121 ggctctaccc gaaactggcg cacttcgccg ccaatcggaa tcacctgcgc cacgcccgga
    15181 atcgacagca ggcgcgggcg cagcacaaag tcggcaaatt cgcgcacctg catcgggctg
    15241 acctgctgag ggtcggccgg cagggcaatc agcatgattt cgcccatcac cgaggcaatc
    15301 ggccccagcg tcgggctgac gcccggcggc agttgctcgc ggatttccgc cagccgttcg
    15361 gccagcatct gccggttgcg gtaaatatcc gagccccagg caaactcggc gtacaccacg
    15421 gataaaccgg cgccagacac cgagcgcacg cgctgcacgc cggcaatgcc attcagcgcg
    15481 gcttccagcg gctgggtgac gcgcagctcg acatcttccg gggccatgcc gccggcttcg
    15541 gtcatcaccg tcaccatggg tttgttcagg tcaggaaaaa catccaccgc catgcggccg
    15601 gcgttcatgc cgccccaggc catcatcaac aaggcaaaca ccagcacaaa caagcgattg
    15661 cgcaggctat gctccaccat ccagctcaac attgggcgcg tcctttagcg aatctggttc
    15721 agcagcgcgg cgccggcgct caccacccgg cggccggcgg gcaggtcgcg caccagcaca
    15781 tcctggccat tcagcggggc aatttgcacc ggccaggggc gaaacagctc ggcgctgtcg
    15841 tgcagccaca ccacgggctg gttggcgcta ttgcgctgca cgctggccgc cggcagcgcc
    15901 acgcccgaga caggcgcgcc gtacgccacg cgcagcttca ccgattgccc cagcgccagc
    15961 ggcacatcct gacggccttg cggggcaaac agcagcggca gcgcgccatc acgaaacgcg
    16021 gtggcgctgc ccaccagctg ccagggggtg gcgctgccgg gcaggctggc gcggcccagt
    16081 tgctgggcca tggccgggtc gtgggccagc gcttccaccc gcagcttttg tgggtccacc
    16141 acttcaaaca gcaatgcgcc ggcctccact tgttggccat tcagcgcatt ggcctggctg
    16201 agcacgccgc tggctggcgc gcgcaaggcc agtcgcccac cggctgcggc caaggcggcg
    16261 gcgcgctggc gcaggctggc ggcttcggcg cgctgggcgt cgatgtcgcg cgcggccacg
    16321 gtctcgcgca ggctttccag ccgcgccaac tggcgcgccg cctgggcggc cttgatgtgc
    16381 agttcggcca gctctgcctg ctggctggcg cgctcgccag cgctcaggct ggggcgcaga
    16441 taggccagca actggccttg gcgcacgcgc tggccggcgc tgggcaggcc atccggcccg
    16501 gcctccagcg cgccgctgag gctggcttgc acgcgtccgc cagcgtttgg atccatcacc
    16561 acatggccgt tcagctctac gctggggcgg tgttcgccca gcatgctcag ttgggtgcga
    16621 atgcccaact ggcgttggct ggctttatcc agccacaccc ggccatcggg caggcgttgt
    16681 ggccgcaagg ccagtgatgc ggcagccggg ttgcgcgggg cgctgggcgc agcggcgggc
    16741 gccggggatg ggctgtcgtg cacttcgtcg ccgtgggcgt gcgcccatgg cgccgtcagg
    16801 cacagcgcca gcgccaggag ggtgtgtcgt gggtttaagc gggtcatggc gcagtcctcg
    16861 tggccttgtg ccggcgacgc caggcccagg cggcggccag ggtcagcgcg atgacggcaa
    16921 ccgcgccgcc tgccatgctc ggcgtgaggg aggccgcgct ggcggcgggc gcggccgtcg
    16981 cgggcaaggt cagcgtggcg gccagcaagt cggcttgctg gccggcttca agggtgaaac
    17041 tcagcgggtg ggcgccgggc tggttgagcc agggggcctt gagcaggtat tcgccgggcg
    17101 cgtgttcgcg cgcctgggct ttccagctgg tgccgcgctc aacttcaatg cgcgcgccgc
    17161 tgatgggcgc attgctggca aagtcgtcca gatacaggcg cagttgctcg ccttcggcga
    17221 caatcagcag ctcgaacact tcgctatggg cgtcggcgcg cggcgtcacg gcttggccca
    17281 gtgcgggcgg ggcggtgtcg tgtgattcgt cgccatgcgc ccaggcggcg gcggacagca
    17341 gccaggcggt taaaacaaac agcgtgcgcc agatcatggc agggctccaa tggcttgcag
    17401 ataacgggag tgcgcgcgcc cggcttccag ttgggcgcgc tgggcttggc tgcgggcttc
    17461 gtgcagttcg ctttgcgcgc gccattgctc gggcgcgctt aattggccgg cgcgcacggc
    17521 gcgctgaatc cagcccagcc gctgttcggc cagttgcaag gcttcggcct gggcgcgggc
    17581 ttgttgctgg cccaattcgc gcgccagacg cgcgctgtcg atgtcttgcg ccagttgctc
    17641 gcggccggcg tgcagctcgg ccagcgcttc gctcagttca acattggcct gcgccatgcg
    17701 cgggccattg cgcgcctggc cgccaaacgg gattttcagc gagagtttgg ccaggttttg
    17761 gtagggttcg ctgcgcgcgc cgcgctcgcg ggtgagggtg agcgccagct cgggggcgtc
    17821 gcggctgtcg ccattggcct gcgccagccg cgcctgggcg gcggcaacgc gcgcatccag
    17881 gctggccagc agcggatggg cgtcgggcag ggcgctgggg tgcgccggct cggcctgctg
    17941 cggcaacgcc gcgccgcgcg cggcggcggt gaattgattg agcgcgcggc gcgcctgcgc
    18001 ctgcgcgcgc agccagtcgg tttcggcctg cgcgcgcgcg ccggcggcct ggttgtgcgc
    18061 cagcggcgct tcctggccgg cgttcacccg ccgcatggcg tcggcttcca gcgcgcgctg
    18121 ggtgtgcaga cgctgctggg cggcggcggc gtccagctca gccagccgcg cctcccacca
    18181 ggcttcgcgc agcgcgcccg ccaactgcca gcgcgccagc gcgcgagcgt gctgctcggc
    18241 gtcctgttcg cgctcggcca ggcggcgggc cagctcgcgc tgcccccaca gccacaaggg
    18301 cgcgctgagc tcgacttccc attcacgcag gccggcgttg cggtccaggc ggtcgctgcg
    18361 ttgcgagagg gtcagcgccg gcgggccggg cagccaggcg tcgctggcct cccgctggct
    18421 atcccgcagc tgctgacgcg cctcgctcac cgcttggcgc gccgcccagg cgcggtcaaa
    18481 cgcctcagcc aggctggcgg cctgtgcggg cgcggccaga cccagccata cggccagggc
    18541 caggcggcaa aaagaagacg cttgcatgga aattccactc aaaaatggct tggcgtgcag
    18601 tattggcggc gcaggccgtc ggcaagctga cgataaaatg acatttttgt catctgcgcc
    18661 cacgcgggcc tcaggcaggg tataagcagc acagacaagg aggctgaatg cgaatcttga
    18721 ttgtggaaga cgaagtgcgc gccnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    18781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    18841 nnngcacaga caaggaggct gaatgcgaat cttgattgtg gaagacgaag tgcgcgccgc
    18901 cgcctatttg cgccagggct tgagcgaagc tggctttagc gtggaggtgg cgcacgatgg
    18961 cgtggagggc gagcacctgg cgctgacgca ggcgtttgac ctgctgattc tggatgtgat
    19021 gctgcccggg cgcagcggct gggagattct ggccagcgtg cgccggatga gccagacgcc
    19081 cgtgttgttt ttgaccgcgc gcgaccgggt ggaagaccgc gtgcgcgggc tggaagccgg
    19141 cgccgatgat tatttggtca agccgtttgc tttttccgag ttgctggcgc gggtgcgcac
    19201 cctgttgcgc cgtcgcccgc aggccgagcc ggatgtgttg cgcgtggccg atctggagct
    19261 ggatttggcc cggcataaag cctggcgcgg cggcgtgcgg gtgacgctga ccgccaagga
    19321 aatggccttg ctggcgctgt tcatgcgtcg gcgcggcgag gtgctgtcgc gcacgctgat
    19381 tgccgagcag gtgtgggaca tgcactttga cagcgacacc aatgtggtgg atgtggcggt
    19441 gcgccggctg cgcgccaaga ttgacgagcc gttcgagctt aagctgattc acaccttgcg
    19501 cggcgtgggc tatgtgctgg aggccggccg tgaagactga ttggcggcgc gcctggcgtg
    19561 ggctgacgcc gcgcgtggtg ctgggttttg ccgcgctgac agcggcgctg atggtggcgt
    19621 gcggggtgta tttgtatcag gcgctgcggc tggagctggg cgcgcgcgac cgcgacgagc
    19681 agcgcggcaa gatcgtgttg tttcgccagg cgctggccgg cgtgtcggac gcgggcatgc
    19741 tgcggcgcaa cccggactgg ctgacgcatt ttatggtggg gcatgacggc ctggcgctgc
    19801 gcgtgctgga tgcgcgcgca gcggtgttgg tggacagccc ggccgccttg ccgcccggcc
    19861 tgtggcgcgg gctggatgaa acgccggccc gcctgagcgg ccacgatgcc cacggccacc
    19921 cctggcaggg gctggcggcg cgcgccacgc tggccgatgg ccaagcggtg cagctggaaa
    19981 tctggcgcga tggccacgat caagaagcac tgctggcgca ataccgcaac catttgctgt
    20041 gggccggcgc gctggccacg ctggtggcgg cggcgctggg cggcgcgctg gtgcgccata
    20101 gcctgcgccc actggcggcg ctcaccgccg ccgccatggc cattcgcccg gataccttaa
    20161 accaacggct ggacgccagc ctggcgcccg ccgagctctt gggcctggta gatgccttca
    20221 atgatgcgct ggcgcggctg gatgcggcgt ttgcccgcct gtcggattat gccgcagatc
    20281 tggcgcatga aatgcgcacg cccctgggca ttttgctcgg acaaacccag gtggccttgt
    20341 cgcgcgggcg cagcctggcc gaataccagc aagtgctggc cgacaatgcc gaagaattgg
    20401 aacggctggc gcgcacggtg aacgacttgc tgtttttggc caaaaccgaa cacagcgaag
    20461 ccgcgctggc ttgcgagtgg ctggatgtgc gtcaggaggt ggacgaggtg tgcgagtttt
    20521 ttgccctgct ggcggaagag cgcgacattc aggtgcaggt ggagggcgcg ctgcgcctgc
    20581 cggtggcgcg cgccgccttg cgccggctgt tgaacaatct gctcagcaac gccatccgct
    20641 acagcccgcc gggcagtgtg gtgcgggtgg cgctggacgc ccgtctgcct ggcgtggcgg
    20701 tgaataaccc gcccgccgcc cccctgcccg agccgctgac gatgttgttt gagcgttttt
    20761 acagccaggg cgacggcaat ggccatggcc tgggcctgcc gattgcccgc gccattgccc
    20821 gtcagcatgg cggagaactg cgcgccagcc tgggcgacga cggccggctg acgctggcgg
    20881 cgcaactggc ggccaaggcc agcctggttc aggcgcgccg atgcagctga aacgcccggt
    20941 tcagcgcttg cagctgatgg gaggcggcca gcacgtcttg cgccgcgcgg gtggcgctgt
    21001 gcgccagctc ggtgttgcgc tccaccagtt gggtgacttg ctgcatattg ccggccacct
    21061 cggcgctggc ggcgccttgc tgggcggcgg cggcggaaat atgcgccgcc atgccggcca
    21121 cttcttcgct ggcgcgctcg atggcgtcca gcccggccac gctggcgtcc agcgcgctga
    21181 cgctgtgctc caccgcctgg gcggcgccgg ccatgctgct gaccgcgctg gcggtggtgg
    21241 cgcgcacttc gttgacggtg gcggcgatgt ccaccgtggc ggcggtggtg cgctcggcca
    21301 gcttgcgcac
//
LOCUS       DAOPYX010000011        20479 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_2697_length_20479_cov_9.349638, whole genome shotgun sequence.
ACCESSION   DAOPYX010000011 DAOPYX010000000
VERSION     DAOPYX010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 20479)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20479)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20479
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2697_length_20479_cov_9.349638"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            118..1383
                     /gene="ispG"
                     /locus_tag="PKN55_01245"
                     /gene_synonym="gcpE"
     CDS             118..1383
                     /gene="ispG"
                     /locus_tag="PKN55_01245"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009116998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HNX69899.1"
                     /translation="MSLTPIIRRASRQVWIGHVPVGGDAPVMIQSMTNTDTADAAATA
                     QQVFELAMAGSEVVRITVNTAEAAAAVAEIRQRLDDLGCNVPLVGDFHFNGERLLRDY
                     PDCARALAKYRINPGNVGKGAKGDEKFAFMIQQAIEHDKPVRIGVNWGSLDQSLAVRL
                     MDENNRRAQPLPPDAVMREALIVSALDSADKAMALGLAADKILLSCKVSHVQDLIAVY
                     RELARRCDFPLHLGLTEAGMGSKGIVASSAALAVLLQEGIGDTIRISLTPEPGAPREK
                     EVIVAQELLQTMGLRAFTPLVTACPGCGRTTSTFFQELAARIQSWLREQMPQWRQQYP
                     GVETLNVAVMGCVVNGPGESKLADIGISLPGTGEVPVCPVYVDGEKTVTLKGEGIADE
                     FQRIVADYVASRYGEGGPKRRKIVPIQPA"
     gene            1438..2712
                     /gene="hisS"
                     /locus_tag="PKN55_01250"
     CDS             1438..2712
                     /gene="hisS"
                     /locus_tag="PKN55_01250"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012696171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HNX69900.1"
                     /translation="MAEKIQAIRGMNDILPDESHQWEFLEHTVRNWLSDYGYRNIRMP
                     IVEDTRLFVRSIGEATDIVEKEMYTFTDSLNGDSLTLRPEGTASCLRAVTEHNLLYNT
                     TQRLWYVGPMFRHERPQKGRYRQFHQIGVEALGFNGPDIDAELILMTADLWQRLGLAP
                     YLKLHLNTLGSSAERAEHRQALIAYLEQHKDALDEDGQRRLYSNPLRVLDSKTPSMQA
                     ICDNAPRLIDHLGEASRRHYDDWRAMLQAVGIDYVENPRLVRGLDYYNLSVFEWVTTE
                     LGAQGTVCGGGRYDSLIEQLGGKPAPAVGFAMGVERLMLLLQDKALLPAAPAPDVYLV
                     NQGEGAALYALTVAQALRAAGLKVIQHCGEASFKSQFKKADASGAAVAVVLGDNEVAQ
                     QQAVVKPLRGGGEQTTVARAELAAAVAAHLNR"
     gene            2724..3359
                     /locus_tag="PKN55_01255"
     CDS             2724..3359
                     /locus_tag="PKN55_01255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012696172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNX69901.1"
                     /translation="MAFDLQEQEQIDELKAFWRKWGTLITGVLVAAALGTAGVQGWRW
                     YQHRQAESAAALYGQMEALAAKGDTAKLVSAAEPIKQEYPRTAYAARAALAAARAAYE
                     ANDAKTAREQLNWVLEHASEPGLKAVARLRLATLAVDGKQYDEALGLLGGEHDTAFDG
                     LFLDAKGDALAAKGDTAGARSAYREALNKTPADAPNRSYLQIKLDALGGES"
     gene            3359..4519
                     /gene="bamB"
                     /locus_tag="PKN55_01260"
     CDS             3359..4519
                     /gene="bamB"
                     /locus_tag="PKN55_01260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HNX69902.1"
                     /translation="MNMPAGLRRVATVAALAAMLGGCSTVTGWFTGSDNAPKPTPLQA
                     IQNNVTPKVRWDVSLGGSADYAFTPALMGDVVWAAGRDGQVVGVDVNTGRQVGRVDTR
                     LPLSGGIGAKNGALYVATTEGELVSLDENGKIRWRSRLTSQALEAPQSDGQRVLVRTN
                     DGRLSAFDADSGKSLWTFQRPQPTLTVRNYGSTTLVGGEAVLLGMPGGRLAVIGANMG
                     DLLWEASVSTPRGASELERMADVASRPVFDRGQVCAVAFQGKLTCFDARSGTPMWSRD
                     VGSSQGLTVDAVNLYVTGDDGTVWAFDRATGRSVWTQNKLKYRNVSAPAMLDRFVLVL
                     DGEGIAHLLSNESGEFVGRQSVAGGKTQTQPQSLGDRVLVQTQGGRVLALSL"
     gene            4650..6038
                     /gene="der"
                     /locus_tag="PKN55_01265"
     CDS             4650..6038
                     /gene="der"
                     /locus_tag="PKN55_01265"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003790541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HNX69903.1"
                     /translation="MKPTIALVGRPNVGKSTLFNRLTRSRDALVADIPGLTRDRHYGH
                     GRGASKPYLVVDTGGFEPIVTDGIMHEMARQTLQAVDEADAVIFLVDARSGATPHDKI
                     IANQLRQLGRPVYLVVNKAEGLRRDMAGAEFHELALGEPWAISGAHGDGVRELIEDVL
                     EHFPDASEDEDARQHPKFAVIGRPNVGKSTLVNAILGEERVIAFDQAGTTRDSIYIDF
                     EREAKPYTIIDTAGVRRRGKVDEMIEKFSVIKTLQAIEDANVVVLVLDAQLDVSEQDA
                     TLAGFALEAGRALVVAVNKWDGLDDYAKDCVRREIDRKLNFLGFAKFHFISALHGKGV
                     GDLFRSIDQAYQAAMVKLSTPRLTRALDVITERQAPPRNGMSRPKMRYAHQGGVNPPV
                     VVIHGSNLVNIPDSYTRYLEHSLCKLFKLEGTPLRVQYKSTDNPYAERKQVSVHPGRR
                     DPVKPGKQFRKK"
     gene            6138..6392
                     /gene="hfq"
                     /locus_tag="PKN55_01270"
     CDS             6138..6392
                     /gene="hfq"
                     /locus_tag="PKN55_01270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018747379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HNX69904.1"
                     /translation="MSAKGQMLQDPFLNILRKEHIPVSIYLVNGIKLQGQIESFDQYV
                     VLLKNTVTQMVYKHAISTVVPARPVSVPYENPAAAPQANS"
     gene            6514..7662
                     /gene="hflX"
                     /locus_tag="PKN55_01275"
     CDS             6514..7662
                     /gene="hflX"
                     /locus_tag="PKN55_01275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014088085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HNX69905.1"
                     /translation="MFERHEFGDRAVVVCLDFGLADFAESVAEAVELVRGAGVTVLSV
                     IQAKRSRPDAALFAGKGKVEEIASEVRGLEANVVIFNHQLSPAQQRNLEREFQCRVVD
                     RNSLILDIFAQRAKSHEGKLQVELAQLDYLSTRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLGKRVKLLRDRLATVQKQRQVQRRSRSRSGVMSVSIVGYTNAGKSTLFNALT
                     KAGAYAADQLFATLDTTSRRLYLNANCSVVLSDTVGFIRDLPHSLVAAFRATLEETVH
                     ADLLLHVVDSANDQRDRQIEDVNKVLAEISADGIPQLLVLNKTDLKGLPGGIERDENG
                     NLHAVRVSALTGEGLDALRAALVELAPAPSFSAGPAYDIHDTSENTWQ"
     gene            7653..8882
                     /gene="hflK"
                     /locus_tag="PKN55_01280"
     CDS             7653..8882
                     /gene="hflK"
                     /locus_tag="PKN55_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014088084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HNX69906.1"
                     /translation="MAMNDNGWRGGKRDNNDGPPDLDEIFRSLNQKLSRLLGGRGGQG
                     GGSPDGGRALFKGGASLLALILGGVWLASGFYVVDAREVGVVLRMGSFNRVSDSGLQW
                     HAPYPFEKVEIVTLTEVRSVEVGYRGNAKNRIPEESLMLTEDQNIIDVQLSVQYDVKD
                     PKAFLFNNATGDRDAKDIVKQATETAIREVVGRNKVDFVLNEGRAQVASETAALIQQT
                     LDRYNLGVRVSKVNINDVQPPEQVLAAFDDAVKASQDREKLRNEGQAYANDVIPKASG
                     LASRLMEEAEGYKQRVVSNAEGDAARFKQVLAEYSKAPGVMRERLYQDMMQQILSNSS
                     KVLVDQKSGSNLLYLPLDKLIQQTHPQQAGAAPQAGAPAANLPPPPTTEPASSAPAGA
                     NRSSSRDVVRSREFEER"
     gene            8886..9770
                     /gene="hflC"
                     /locus_tag="PKN55_01285"
     CDS             8886..9770
                     /gene="hflC"
                     /locus_tag="PKN55_01285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018149903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HNX69907.1"
                     /translation="MDRLIPSLIALLAVLLVLSLSLFTVDQRQYAMVFQFGEVVRVIK
                     EPGIQFKIPMLQNVRYFDRRIQTIDPDSPELFNTREKKNVLVDSFVKWRVVDVEQFYK
                     SVGGNEAAAVSRLRQTINDGLRAEFGQKTVTDVISGQRDQVMESVRKRADLDARKIGV
                     QLLDVRLKRVDFPEKISQSVYDRMQSERRAVASQQRSEGAAEAERIRADAERQRDIIL
                     AEAYRKAQAIKGEGDARAAAIYAEAYGKNPEFYAFYRSMDAYKQTFRNKSDVLVLDPS
                     SQFFKYLKNPGAGAKATQ"
     gene            9846..11000
                     /locus_tag="PKN55_01290"
     CDS             9846..11000
                     /locus_tag="PKN55_01290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014088082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HNX69908.1"
                     /translation="MRNWLLPENIADILPATARQVESAKAAMLERFRTYGYELVCPPL
                     IEYIDSLVYDGDTALVMKTFKLDDQMSGRQLGLRADITPQVARIDAHLLSSRQGVTRL
                     CYAGSVLHTVPESAMRSREPLQVGAELYGSSDVGADLEIVDLMLASLEGVGVGGLTLD
                     IGHVGIFRALAAAAALPARLTAELFGVLQAKDIPSAETLLAGAAEPFRSALLALPGLY
                     GPDALSRASQCLPALPEIRQALADMQAVADSVAGRATVNFDLSDLRGTAYHTGLMFAA
                     YAPGWSDALARGGRYDNVGDKFGRARPATGFSLDLRDLVRVLPQRSPAMGIRVAGADA
                     GRAREAIAALRAAGEVLVIDYLGESAAALNCDRALVWRADGWQVEPFASH"
     gene            11027..12322
                     /locus_tag="PKN55_01295"
     CDS             11027..12322
                     /locus_tag="PKN55_01295"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011137075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HNX69909.1"
                     /translation="MSKNVVVIGTQWGDEGKGKIVDWLTDHAEGVVRFQGGHNAGHTL
                     VVNGKKTVLRLIPSGMLHPGKTCFIGNGVVLSPEALLKEIDELQAAGIDVASRLKISE
                     ACPLILPYHIALDKAREAAKGENKIGTTGRGIGPTYEDKVARRAIRVQDLFYPERFAA
                     KLGENLAYYNFLLKEYFKTDTVDFQETLEQTMQLAERIKPMVADVSRTLYELNKSGKS
                     ILFEGAQGTLLDVDHGTYPFVTSSNCVAGAASAGAGVAPQMLNYVLGIVKAYTTRVGS
                     GPFPTELFDDVGAGLAKRGNEFGAVTGRPRRCGWFDAALLKRSIQINGVSGLCVTKLD
                     VMDGMDEIRLCVGYKKDGQTYDILPFGAENVAGCEPIYETWPGWSETTFGVKRYEELP
                     ENARNYLQRISEFCEAPIDIISTGPDREETIVLRHPFGA"
     gene            12647..13150
                     /locus_tag="PKN55_01300"
     CDS             12647..13150
                     /locus_tag="PKN55_01300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX69910.1"
                     /translation="MSVKPAVKHELHACQQQVSLLLDSQREQRSIGMDSETLQPDALL
                     AFFTERNLPFAYYVRSCSGIAIGEASAYEKNIATLNMYMAHLRATEKAQIDNTIATLN
                     EYKSRNQAIGLSADTLRPDRFMSFFAVRELPFAMYVPQGERALGDPSAYERNIRVLEH
                     SLATLQA"
     gene            13234..14394
                     /gene="zapE"
                     /locus_tag="PKN55_01305"
     CDS             13234..14394
                     /gene="zapE"
                     /locus_tag="PKN55_01305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012696165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HNX69911.1"
                     /translation="MSQHAFSPDKHTTLSPRQWYEDAAQKPGFIRDAAQSMAIDHLDA
                     LYQELLVFKKKRHSFLGKSLRKPSIPRGLYFWGGVGRGKSFLMDAFYACVPYRRKRRI
                     HFHHFMMEVHNELKTLTNATDPLITVAERIAQKTRLLCFDEFHVSDIADAMILGRLMK
                     ELFERGVICVLTSNYPPDELYPNGLQRINFLPAIELLKNWLTVLNVDGGNDYRLRELT
                     REPLFITPLKGSRDKLDALYERLSSGTGKELKHEIIVFDRKIRAIRHSPGAIWFDFME
                     ICGGPRAQTDYLEIAREYQTVFVSNVPKLAPHQSSEARRFTWLVDVFYDHRVKLVLSA
                     EVEAEQIYTDGVQSSEFFRTASRLTEMQSTSYLGLPHLSDVFEHTPAANLAE"
     gene            complement(14431..15111)
                     /gene="rpe"
                     /locus_tag="PKN55_01310"
     CDS             complement(14431..15111)
                     /gene="rpe"
                     /locus_tag="PKN55_01310"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012696677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HNX69912.1"
                     /translation="MRTFRIAPSILSANFACLGQEVTDVIAAGADIIHFDVMDNHYVP
                     NLTIGPLVCEAIRPLTEAPIDVHLMVKPVDSLVPMFAKAGANLITFHPEGSEHVDRTL
                     ALIRDCGCKAGLVFNPATPLHHLDHVMDKLDMILIMSVNPGFGGQSFIPQALNKLREA
                     RRRIDAYREATGRDIWLEVDGGVKVDNIAEIAAAGADTFVAGSAIYGADDYAATIAAM
                     RAELAKVA"
     gene            15444..15815
                     /gene="apaG"
                     /locus_tag="PKN55_01315"
     CDS             15444..15815
                     /gene="apaG"
                     /locus_tag="PKN55_01315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018749616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HNX69913.1"
                     /translation="MTSPIRVEPQAYYIADQSNPANHCYVFAYRIQIHNDGDVGVQLI
                     SRSWYITDANNHVREVHGEGVVGEQPRLQPGETYEYVSGAVLATAQGTMRGSYNFTAD
                     DGREFEAPVPEFFLNGAQALH"
     gene            15915..17699
                     /locus_tag="PKN55_01320"
     CDS             15915..17699
                     /locus_tag="PKN55_01320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012696886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HNX69914.1"
                     /translation="MRFTHSGPAPTERNDAKTLKTLLPYLLEFKGRVALALSLLMLAK
                     LANVSVPWFLKDIVDQLSATQAALAMPLGLVAAYGLARLATSVFGELRDAVFAKVTQR
                     AIRRIALQVFEHLHRLSLRFHLERQTGGMSRDIERGTKGIGFLLNFTLFNILPTLVEI
                     GLVAGILLVNYHWSFAVVTFLTIASYIGFTLGVTEWRMVFRRSMNELDSQANSKAIDA
                     LLNYETVKYFGNERYEVARYDGNLASWERSAVRNQTSLNFLNAGQGVIIAVGVTVLVG
                     LAARGVVDGSMSVGDMVLVNAYLIQLYAPLNFLGFVYREIKHSLADMERMFTLLEQGE
                     EVADRPGAQTLATRQAAIRFEQVQFGYDGKRQILHDVSFDIPAGKTVAVVGASGAGKS
                     TLSRLLFRFYDVGAGRITLNGVDIRELTQASLRAHIGIVPQDTVLFNDSIYYNIAYGR
                     PDASREQVIEAARAAHIHDFVSQLPDGYDTVVGERGLKLSGGEKQRVAIARTLLKNPP
                     ILIFDEATSALDSHTEKAIQAELAEIAAQRTTLIIAHRLSTVVDAHQIVVMEGGRIIE
                     QGSFRDLLDRQGRFAQMWQLQLQDADHA"
     gene            17692..19173
                     /locus_tag="PKN55_01325"
     CDS             17692..19173
                     /locus_tag="PKN55_01325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012697215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-histidine dipeptidase"
                     /protein_id="HNX69915.1"
                     /translation="MPEPTLPSLPLDLPPAALWRHFATLCRLPRPSRHEGQVMDHLCR
                     WASTRSLNWTQDAAGNLLIRKPASPGMAERETVVLQGHVDMVCQQDGDAHDFFSDPIQ
                     PWLADDGWVRARGTSLGADNGIGVAAMLAVLESDDIAHPPLEALFTVNEEAGMDGAHD
                     LAEDLLTGRFLLNLDTEDWGEFYIGCAGGEDVILRRQCEREPLPPEHHAWRLTVRGLC
                     GGHSGVDIHRYRANATRLLARLLSELAGRFGLRVGQLAGGALRNVIPREASATLAVPE
                     SKLEALQHTLLDWQKRVAATLPEWDHGLTVTLGPAQLNAVLSLHDQAVLIDLLNALPD
                     GMAGLSPALPDVVHTSSNLGVVSLDNGRFQAVSMVRSLSDDGLESRVASLLACGRLAG
                     CSGVREGFYPGWTPRPESPLLALASQVYQRQHGQRPKVKVIHAGLECGILLGKYPDLD
                     IISFGPDIRGAHSPSERVNVASVARFWDFLCELLAQIPARRLD"
     gene            complement(19266..>20479)
                     /gene="pnp"
                     /locus_tag="PKN55_01330"
     CDS             complement(19266..>20479)
                     /gene="pnp"
                     /locus_tag="PKN55_01330"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018747708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HNX69916.1"
                     /translation="ILAGEPRIDGRDTRTVRPISIRTGVLPRAHGSALFTRGETQALV
                     VTTLGTKQDEQVIDALAGEYTERFMLHYNFPPYSTGETGRVGSPKRREIGHGRLAKRA
                     LVAALPPADEFSYSMRVVSEITESNGSSSMASVCGGCLAMLNAGVPLKDHVAGIAMGL
                     ILEGNRFAVLTDILGDEDHLGDMDFKVAGTANGVTALQMDIKIQGITKEIMRVALDQA
                     KEGRLHILGLMKNAVDAPQELSAHAPRLYTMKINPEKIRDVIGKGGSVIRSITEETGT
                     SIDIGEDGLITIASVSGEGAEAAKRRIEQITAEVEIGKIYEGTVMKILDKNVGAIVQI
                     MPGRDGLVHISQIANERIANVGDYLKEGQQVRVKVLETDERGRIRLSIKAVLNEETAA
                     AAPAPSEAAAQ"
BASE COUNT         3756 a         6411 c         6536 g         3776 t
ORIGIN      
        1 gctccgcaag tggagctcaa ttacaagggc caggcggtgc cgctggccga caagaccaag
       61 ggcagcaccg ccaagctgga actgcaatga ccccggatgg cgtgaaccga taaaatcatg
      121 agcctgacac cgattatccg ccgcgcctcg cggcaagtct ggattggtca tgtgccggtg
      181 ggcggcgacg ccccggtgat gatccagtcg atgaccaaca ccgacaccgc cgacgccgcc
      241 gccaccgcgc agcaggtgtt cgagctggcc atggccggct cggaggtggt gcgcatcacc
      301 gtcaacaccg ccgaggccgc cgccgccgtg gccgagattc gccagcgtct ggacgatctg
      361 ggctgcaatg tgccgctggt gggcgatttt cacttcaatg gcgagcgcct gctgcgcgac
      421 tacccggact gcgcacgcgc gctggccaaa taccgcatca accctggcaa tgtcggcaag
      481 ggcgccaagg gcgacgagaa attcgccttc atgatccagc aggccatcga gcacgacaag
      541 ccggtgcgca ttggcgtgaa ctggggcagc ctggaccaaa gcctggcggt gcggctgatg
      601 gacgaaaaca accgtcgcgc ccagcctttg ccgccggacg cggtgatgcg cgaggcgctg
      661 attgtctcgg cgctggacag cgccgacaaa gccatggcgc tgggcctggc cgccgacaag
      721 attttgctgt cgtgcaaggt cagccatgtg caggatttga tcgccgttta ccgcgagctg
      781 gcgcgccgtt gtgattttcc gctgcatctg ggcctgaccg aggccggcat gggctcgaaa
      841 ggcattgtcg cctccagtgc ggcgctggcc gtgctgctgc aagaaggcat tggcgacacc
      901 atccgcattt cgctgacccc cgagccgggc gcgccgcgcg aaaaagaagt gatcgtcgcc
      961 caggaactgc tgcaaaccat gggcctgcgc gccttcaccc cgctggtcac cgcctgtccg
     1021 ggttgcgggc gcaccaccag caccttcttt caggagctgg cggcgcgcat tcaatcctgg
     1081 ctgcgcgagc agatgccgca gtggcggcaa caataccccg gcgtggaaac cctgaatgtg
     1141 gcggtgatgg gctgtgtggt gaacggccct ggcgaatcca agctggccga cattggcatc
     1201 agcctgcccg gcactggtga agtgccggtg tgcccggtgt atgtggacgg cgaaaaaacc
     1261 gtcaccctca agggcgaggg catcgccgat gaattccagc gcatcgtcgc cgattatgtg
     1321 gccagccgct acggcgaagg cggccccaag cggcgcaaga ttgtgcccat ccagccggcc
     1381 tgagcgccgc gctgatccgc ctaccgaaca gctattgcat tgactgaaaa aaacaacatg
     1441 gcagaaaaaa tccaggcaat tcgcggcatg aacgacatcc tgccggatga atcgcaccag
     1501 tgggaattcc ttgagcacac cgtgcgcaac tggctgtccg actacggcta ccgcaatatc
     1561 cgcatgccga ttgtcgaaga cacccgtctg tttgtgcgct cgattggcga ggcgaccgat
     1621 attgtcgaga aggaaatgta caccttcacc gacagcctca acggcgacag cctgaccctg
     1681 cgcccggaag gcaccgcctc gtgcctgcgc gcggtcaccg aacacaatct gctgtacaac
     1741 accacccaac ggctgtggta tgtcggcccg atgttccgcc acgagcgccc gcaaaaaggc
     1801 cgctatcgtc agtttcacca gatcggcgtg gaagcgctgg gcttcaacgg cccggacatc
     1861 gacgccgagc tgatcctgat gaccgccgac ctgtggcagc gcctgggcct ggccccctac
     1921 ctcaagctgc acctgaacac cctgggctcc agcgccgagc gcgccgagca tcgtcaggcg
     1981 ctgattgcct atctggagca gcacaaggac gcgctggacg aagacggcca gcgccgcctg
     2041 tacagcaacc cgctgcgcgt gctggacagc aaaaccccgt cgatgcaggc catctgcgac
     2101 aacgccccgc gcctgatcga ccacctgggt gaggccagcc gccgtcacta cgacgactgg
     2161 cgcgccatgc tgcaagcggt gggcattgat tacgtggaaa acccgcgtct ggtgcgcggc
     2221 ctggattact acaatctgtc ggtgttcgag tgggtcacca ccgaactggg cgcgcaaggc
     2281 acggtgtgcg gcggcggccg ttacgacagc ctgatcgagc agctgggcgg caagccggcg
     2341 ccggccgtgg gctttgccat gggcgtcgag cggctgatgc tgctgttgca ggacaaagcc
     2401 ctgctgccgg cggcgccggc cccggacgtg tatctggtca accagggcga aggcgcggcg
     2461 ctgtatgcgc tgactgtggc ccaggcgctg cgcgccgctg gcctgaaggt gatccagcac
     2521 tgcggtgaag ccagcttcaa atcgcaattc aaaaaagccg acgccagcgg cgcggcagtg
     2581 gccgtggtgc tgggcgacaa tgaagtggcc cagcaacagg ccgtggtcaa gccgctgcgc
     2641 ggcggcggcg agcaaaccac cgttgcccgc gccgagctgg ccgccgcagt ggccgcccac
     2701 cttaaccgct gaacgaggaa gccatggcgt ttgatcttca ggaacaggaa cagattgacg
     2761 aactcaaggc cttctggcgc aaatggggca cgctgattac cggcgtgctg gtggccgctg
     2821 cgctgggcac ggccggtgtg cagggctggc gctggtatca gcatcgccag gctgagtcgg
     2881 ccgctgcgct gtacgggcag atggaagcgc tggcggccaa gggtgacacc gccaagctgg
     2941 tgagcgccgc cgagccgatc aagcaggaat atccgcgcac cgcctacgcc gcccgcgccg
     3001 cgctggccgc tgcccgtgct gcttatgaag ccaacgacgc caagaccgcc cgcgaacagc
     3061 tgaactgggt gctggagcac gccagcgagc ctggcctgaa ggcggtggct cgcctgcgcc
     3121 tggccacgct ggcggtggat ggcaagcaat acgatgaggc attgggcctg ctgggtggcg
     3181 agcatgacac cgcctttgac ggcctgttcc tggacgccaa gggtgatgcc ctggcggcca
     3241 agggcgatac tgctggcgct cgctccgcct atcgtgaagc actgaacaag accccggctg
     3301 atgcgccgaa ccgatcctat cttcaaatca agcttgatgc gctgggaggc gaatcttgat
     3361 gaacatgccc gcaggtctgc gccgtgtggc gaccgtagcc gcactggccg ccatgcttgg
     3421 cggctgctcg actgtcaccg gatggtttac cggcagcgac aatgccccca agccgacccc
     3481 gctgcaagcc attcagaata acgtgacgcc gaaagtgcgt tgggacgttt cgctgggcgg
     3541 ttctgcggat tatgccttta ccccggcctt gatgggcgat gtggtgtggg ctgctggccg
     3601 tgatggccag gtggtggggg tggatgtcaa cactggccgg caagtgggtc gggtggacac
     3661 ccgtctgccg ctgtcgggcg gcattggcgc caagaacggc gcgctgtacg tggccaccac
     3721 cgaaggcgag ctggtgtcgc tcgacgaaaa cggcaagatt cgctggcgca gccgcttgac
     3781 cagtcaagcg ctggaagccc cgcaaagcga tggccagcgc gtgctggtgc gcaccaacga
     3841 cggtcgcctg tcggcatttg atgctgacag tggcaagtcg ctgtggacct tccagcgccc
     3901 gcagccgacg ctgaccgtgc gtaattatgg ctccaccacc ctggtgggcg gcgaagccgt
     3961 gctgctgggc atgccgggcg gccgtctggc ggtgattggc gccaatatgg gcgatctgct
     4021 gtgggaggct tcggtctcca ccccgcgcgg cgcttccgag ctggaacgca tggccgatgt
     4081 ggccagccgc ccggtgttcg accgtggcca ggtgtgtgcg gtggccttcc agggcaagct
     4141 gacctgcttt gacgcgcgca gcggcacgcc gatgtggtcg cgcgatgtcg gctccagcca
     4201 ggggctgact gtggacgcgg tcaatctgta cgtcaccggt gacgatggca cagtgtgggc
     4261 gtttgaccgg gccactggcc gctcggtgtg gacgcagaac aagctgaaat accgcaacgt
     4321 cagcgcgccg gccatgttgg accgttttgt gctggtgctg gacggcgagg gcattgccca
     4381 cttgctgtcg aatgaatccg gtgaatttgt cggccgccag tcggtggccg gcggcaagac
     4441 ccagacccag ccgcaaagcc tgggcgaccg cgtgctggtg caaacccagg gcggccgcgt
     4501 gctggcgctg agcctgtaac ttactgaacc cacagaggct gcttgcgccg ttcgcggcgc
     4561 cgcctgcccc gtcacggccc gccgggagtt gtcccgccgg gtcgtctggc gctggtgctg
     4621 gcaagtgcag cccgcaagga aaaatggcag tgaaacccac tatcgcgctg gtcggacggc
     4681 ccaatgtcgg caaatcgacg ctattcaacc ggctcacccg cagtcgcgac gccctggtgg
     4741 ccgacatccc cggcctgacc cgcgaccgcc actatggcca cggccgtggc gccagcaaac
     4801 cgtatctggt ggtggacacc ggcggttttg aaccgattgt taccgacggc atcatgcacg
     4861 aaatggcgcg ccagacgctt caggccgtgg acgaagccga cgcggtgatt tttttggtgg
     4921 acgcccgctc gggcgccacg ccgcacgaca agatcatcgc caaccagctg cgtcagctgg
     4981 gccggccggt gtatctggtg gtgaacaagg ccgaaggcct gcgccgcgac atggccggcg
     5041 ccgagttcca cgagctggcg ctgggcgagc cgtgggcgat ttctggcgcg catggcgacg
     5101 gcgtgcgtga gctgatcgaa gacgtgctcg agcacttccc cgacgccagc gaggacgaag
     5161 acgcccgcca gcatcccaaa ttcgccgtga ttggccggcc gaatgtgggc aaatccaccc
     5221 tggtcaacgc cattctgggc gaagagcggg tgattgcctt tgaccaggcc ggcaccacgc
     5281 gcgacagtat ttatatcgat ttcgaacgcg aagccaaacc gtataccatt atcgacaccg
     5341 ccggcgtgcg ccggcgcggc aaggtggacg agatgatcga gaagttctcg gtgatcaaga
     5401 ccttgcaggc catcgaagac gccaatgtgg tggtgctggt gctggacgcc cagctggatg
     5461 tttccgagca ggatgccacc ctggccggct ttgccctgga agccggccgc gcgctggtgg
     5521 tggcggtgaa caaatgggac gggctggacg attacgccaa agactgtgtg cgccgcgaaa
     5581 tcgaccgcaa attgaacttt ttgggctttg ccaagttcca cttcatctct gccttgcacg
     5641 gcaagggcgt gggcgatttg ttccgctcga tcgaccaggc ttatcaggcc gccatggtga
     5701 aattgtccac cccgcgcctg acccgcgcgc tggacgtgat caccgagcgc caggcgccgc
     5761 cgcgcaacgg catgtcgcgt ccgaagatgc gctacgccca ccagggtggg gtgaatccgc
     5821 ctgtcgtggt gatccacggc agcaatctgg tgaatattcc ggacagttac actcgctatc
     5881 ttgaacacag cctgtgcaaa ttgttcaaac tggaaggcac cccattgcgg gttcagtaca
     5941 aatcgacgga caatccgtat gccgaacgca agcaggtcag cgtgcatccg gggcgacgcg
     6001 atccggtcaa gccaggaaag cagttcagga agaaataacg cagcaggggc aacggtgctc
     6061 tgtctggcca tcgccgggcg gagtatggaa tccggtgttc gacaacataa caatttaact
     6121 taacctggag tcaatccatg agcgccaaag ggcaaatgct acaagacccg tttctgaaca
     6181 ttctgcggaa agaacatatt ccggtgtcga tctacctggt gaacggcatc aagctgcagg
     6241 gtcagatcga atcgttcgac cagtacgtgg tgctgctgaa aaacaccgtc acccagatgg
     6301 tgtacaagca cgccatttcc accgtggtgc cggcgcgccc ggtgtcggtg ccgtatgaaa
     6361 acccggctgc cgcaccccag gccaatagct gattggccct cgccctgaat ccatcgaccg
     6421 gccgttgccc tggcgcattc cggttccagc ctgctggccg gaaccacacg cagggtgcac
     6481 cggtcgattt tctttgcagg atgcagctct cccgtgtttg aacgtcacga atttggcgac
     6541 cgtgcggtcg tcgtgtgtct ggactttggc ctggccgatt ttgccgaatc ggtggccgag
     6601 gccgtcgagc tggtgcgcgg cgcgggcgtc accgtgctgt cagtgattca ggccaagcgc
     6661 agccgcccgg acgccgccct gtttgccggc aagggcaaag tggaagaaat cgccagcgaa
     6721 gtgcgcggcc tggaggccaa tgtggtgatt ttcaatcacc agctgtcgcc ggcgcagcag
     6781 cgcaatctgg agcgcgagtt tcagtgtcgg gtggtggacc gcaacagcct gattctggat
     6841 atttttgccc agcgcgcgaa aagccacgaa ggcaaattgc aggtggagtt ggcccagctg
     6901 gattatctgt ccacccggct ggtgcgcggc tggacgcacc tggagcggca aaaaggcggc
     6961 attggcctgc gcggccccgg cgaaacccag ctggaaaccg accgccgctt gctgggcaag
     7021 cgcgtcaagc tgttgcgcga ccggctggcc acggtgcaaa agcagcgcca ggtgcagcgg
     7081 cgcagccgct cgcgctcggg cgtgatgagc gtgtcgattg tcggctacac caatgccggc
     7141 aaatccacct tgttcaacgc cctgaccaag gccggcgctt atgcggcgga tcagctgttc
     7201 gccacccttg acaccaccag ccggcggcta tacttgaacg ccaattgctc ggtggtgctg
     7261 tccgacaccg tgggctttat ccgcgatctg ccgcactcgc tggtggcggc gtttcgcgcc
     7321 acgctggaag aaaccgtgca tgccgatttg ctgctgcacg tggtggacag cgccaatgat
     7381 cagcgcgacc ggcagattga agacgtcaac aaggtgttgg cggaaatctc cgccgacggc
     7441 attccgcagc tgctggtgct gaacaagacc gacctcaagg gcttgccggg cggcatcgag
     7501 cgggatgaaa atggcaatct tcatgcagtg cgcgtatctg cgctcactgg cgaagggttg
     7561 gacgccttgc gcgcggcgct tgtggaactt gcgcctgccc ccagcttctc agctggaccc
     7621 gcttacgata ttcacgatac ttcggaaaac acatggcaat gaatgacaac ggctggcgcg
     7681 gcggcaaacg ggacaacaac gacggcccgc ccgacctcga cgaaattttc cgttcgctca
     7741 accaaaagct ctcgcggctg ttgggcgggc gcggcggcca gggcggcggc tcgcccgatg
     7801 gcggacgcgc gctgttcaag ggcggcgctt cgctgctggc gctgattctg ggcggcgtat
     7861 ggctggcttc tggtttttac gtggtggacg cccgcgaagt gggcgtggtg ctgcgcatgg
     7921 gcagcttcaa ccgtgtatcc gactccggcc tgcaatggca cgcgccgtat ccgtttgaaa
     7981 aagtcgaaat cgtcaccctg accgaagtgc gcagtgtgga agtgggctat cgcggcaacg
     8041 ccaagaaccg cattcccgaa gaatcgctga tgctcaccga agatcagaac atcatcgacg
     8101 tgcaattgtc ggtgcagtac gacgtcaaag acccgaaagc cttcctgttc aacaatgcca
     8161 ctggtgaccg cgacgccaag gatattgtca agcaggccac cgaaaccgcc atccgcgagg
     8221 tggtcggccg caacaaggtg gattttgtgc tcaacgaagg ccgcgcccaa gtggcgtcgg
     8281 aaaccgccgc cttgattcag caaacgctgg accgctacaa cctgggcgtg cgggtgagca
     8341 aggtcaatat caacgatgtg cagccgcccg agcaggtgct ggcggcgttt gacgacgcag
     8401 tcaaggccag ccaggaccgc gaaaagctgc gcaacgaagg ccaggcctac gccaatgacg
     8461 tgattcccaa ggccagcggc ctggcgtcgc ggctgatgga agaagccgag ggctacaagc
     8521 agcgcgtggt gtccaacgcc gagggtgacg ccgcccgctt caagcaggtg ctggccgaat
     8581 acagcaaggc gccgggcgtg atgcgcgagc gcctgtatca ggacatgatg cagcaaatcc
     8641 tcagcaacag cagcaaggtg ctggtcgatc aaaaatccgg cagcaatctg ctgtatttgc
     8701 cgctggacaa gctgattcag cagacccatc cgcaacaagc cggcgctgcg ccgcaagcgg
     8761 gcgcgcctgc ggccaatctg ccgccgccgc cgaccactga accggccagt tccgccccgg
     8821 ctggcgccaa ccgtagcagc agccgcgacg tggtgcgcag ccgcgaattt gaggagcgtt
     8881 gattgatgga tcgtcttatt cccagcctga ttgccctgct ggccgtgctg ctggtgctca
     8941 gcctgtcgct gttcaccgtc gatcagcgcc agtatgcgat ggtgttccag tttggcgaag
     9001 tggtgcgggt gatcaaagag cctggcatcc agttcaagat cccgatgttg cagaatgtgc
     9061 gttactttga tcgccgcatt cagaccatcg acccggactc gcccgagctg ttcaacaccc
     9121 gcgaaaagaa aaacgtgctg gtggacagct ttgtcaaatg gcgcgtggtg gacgtcgagc
     9181 agttttacaa gtcggtgggc ggcaatgaag ccgctgcggt gtcgcgcctg cgtcagacca
     9241 tcaacgatgg cctgcgcgcc gaatttggcc agaaaaccgt caccgacgtg atttccggcc
     9301 agcgcgacca agtgatggaa tcggtgcgca agcgcgccga cctggacgcg cgcaagattg
     9361 gcgtgcagct tttggacgtg cgcctgaagc gggtggactt ccccgagaag atcagccagt
     9421 cggtgtatga ccgcatgcag tccgagcgcc gcgccgtggc cagccagcaa cgctcggaag
     9481 gcgctgccga ggctgaacgc atccgcgccg acgccgaacg ccagcgcgac atcatcctgg
     9541 ccgaagccta ccgcaaggcc caggccatca agggtgaggg cgacgcccgc gccgccgcca
     9601 tctacgccga ggcgtatggc aaaaaccccg agttctatgc gttttaccgc agcatggacg
     9661 cctacaagca aaccttccgc aacaagagcg atgtgctggt gctcgacccc agctcgcagt
     9721 tcttcaaata cctgaagaac ccgggcgcag gcgcaaaggc cacccagtag gacgatttcg
     9781 gattacaata gcggtttgac cattaagcgg gggctcctcc cccgcttttt gctgtgaatt
     9841 ctcccatgcg aaactggctg ctccctgaaa acatcgccga catcctgccc gccaccgcgc
     9901 gccaggttga atcggccaag gcggcgatgc tcgaacgctt ccgcacttac ggatacgagc
     9961 tggtctgtcc tcccctgatc gaatacatcg attcgctggt ctacgacggc gacaccgcgc
    10021 tggtgatgaa gaccttcaag ctcgacgacc agatgagcgg ccgtcagctg ggcttgcgcg
    10081 ccgacatcac cccgcaggtg gcgcgcatcg acgcgcattt gctgtccagc cgccagggcg
    10141 tcacccggct gtgctacgcc ggcagcgtgc tgcacaccgt gccggaaagc gccatgcgct
    10201 cgcgcgagcc gctgcaagtc ggcgccgagc tgtatggctc gtccgacgtt ggcgctgacc
    10261 tggaaatcgt tgacctgatg ctcgcctcgc tggaaggcgt gggcgtgggc ggcttgacgc
    10321 tggacatcgg ccatgtcggc atcttccgcg cgctggccgc agcggcggcg ctgccggcgc
    10381 ggctgaccgc cgagctgttt ggcgtgttgc aggccaagga catccccagc gccgaaaccc
    10441 tgctggccgg cgccgccgag cccttccgca gcgccttgct ggccctgccg gggctgtatg
    10501 gcccggatgc gctcagccgc gccagccaat gcctgccggc gctgccggaa atccgtcagg
    10561 cgctggccga tatgcaggcg gtggcggaca gcgtggccgg ccgcgccacg gtgaactttg
    10621 atttgtctga cctgcgcggc acggcgtatc acaccggcct gatgttcgcc gcctacgcgc
    10681 cgggctggtc ggatgcgctg gcgcgcggcg gccgctacga caatgtcggc gacaaatttg
    10741 gccgcgcccg cccggccacc ggctttagcc tggatttgcg cgatctggtg cgcgtgctgc
    10801 cgcagcgcag cccggccatg ggcattcgcg tggccggcgc tgacgcgggc cgcgcgcgcg
    10861 aggccatcgc cgccttgcgc gccgctggcg aagtgctggt gattgattat ctcggcgaat
    10921 cagccgcagc gcttaattgc gaccgcgcgc tggtctggcg ggcggacggc tggcaagtcg
    10981 aaccgttcgc ttcccattga actgcattgt gtaaagaggt ttcttcatgt ccaagaacgt
    11041 agtcgtgatc ggcacccaat ggggtgacga aggcaaaggc aagattgttg actggctgac
    11101 cgatcacgcc gagggcgtgg tgcgcttcca gggcggccac aacgctggcc acaccctggt
    11161 ggtgaatggc aagaaaaccg tgctgcgcct gattccgtcc ggcatgctgc atcctggcaa
    11221 gacctgcttc atcggcaatg gcgtggtgct gtcgccggaa gcgctgctga aggaaatcga
    11281 cgaactgcaa gccgccggca tcgacgtggc cagccgcctg aagatttccg aagcctgccc
    11341 gctgatcctg ccctaccaca tcgcgctgga caaggcgcgc gaagccgcca agggcgaaaa
    11401 caagatcggc accaccggcc gtggcattgg cccgacctac gaagacaagg tggcccgtcg
    11461 tgccatccgc gtgcaggatt tgttctaccc cgagcgtttt gccgccaagc tgggcgaaaa
    11521 cctggcttac tataacttcc tgctcaagga atacttcaag accgacaccg tggacttcca
    11581 ggaaaccctg gaacagacca tgcagctggc cgagcgcatc aagccgatgg tggccgacgt
    11641 gtcgcgcacc ctgtacgagc tgaacaagag cggtaaatcc atcctgtttg aaggcgccca
    11701 gggcaccctg ctggacgtgg accacggcac ctatccgttt gtcacctcgt ccaactgtgt
    11761 ggccggcgcc gcctctgccg gcgctggcgt ggccccgcag atgctcaact acgtgctggg
    11821 catcgtcaag gcgtacacca cccgcgtcgg ttctggcccg ttcccgaccg aactgttcga
    11881 cgacgttggc gctggcctgg ccaagcgcgg caacgaattt ggcgccgtga ctggccgccc
    11941 gcgtcgctgc ggctggtttg acgccgcgct gctcaagcgc tcgatccaga tcaacggcgt
    12001 gtccggcctg tgcgtgacca agctggacgt gatggacggc atggacgaaa tccgcctgtg
    12061 cgtcggctac aagaaagacg gccagactta cgacatcctg ccgtttggcg ccgagaacgt
    12121 cgccggctgc gagccgattt acgaaacctg gccgggctgg agcgaaacca ccttcggcgt
    12181 gaagcgttac gaagaactgc cggaaaacgc ccgcaattat ctgcaacgga tttccgagtt
    12241 ctgcgaagcg ccgatcgaca tcatttcgac cggcccggac cgcgaagaaa ccatcgtgct
    12301 gcgtcacccg ttcggcgctt aaagctgtct aacaaaaccc tcctggcggt gttgtgtggc
    12361 cttgccgtgc tattgcactg tctgcggcca cacgcctagc caggatcgct gcactgggtt
    12421 ttgtcggcag ttttgctgcg acagcgaatc gctcaacaca ccgcccccgt ccggcttgcc
    12481 cggcgggggc tgtgtttttg gggtgtgcaa taacacgtca ggtttggtca gctgtaatca
    12541 gctgtaatcg ttttcggtgt cggtgttgct tacagtcatg ctcaacatga tctgcgcccg
    12601 ccgtgcttca cggcgcccgc gtggcactga gtgaggagaa cacgccatgt ccgtcaaacc
    12661 tgccgtcaaa cacgaacttc acgcctgtca gcagcaagtg tcgctcttgc tggatagcca
    12721 gcgcgaacag cgttccattg gcatggacag cgaaaccttg cagcccgatg cgctactggc
    12781 gttttttacc gagcgcaatc tgccgtttgc ttactatgtg cgctcttgct cgggtatcgc
    12841 catcggcgag gccagcgcct acgaaaaaaa catcgccacg ctgaatatgt atatggcgca
    12901 cctgcgcgcc accgaaaaag cccagatcga caacaccatc gccacactca acgaatacaa
    12961 atcacgcaac caggccattg gcctgagcgc cgacaccctg cgcccggacc gtttcatgag
    13021 cttttttgcc gtgcgtgagc tgccgtttgc catgtatgtg ccgcaaggcg agcgcgcgct
    13081 gggcgatccg tcggcgtatg agcgcaatat ccgcgtgctg gaacattcgc tggccacgct
    13141 gcaagcctga gcgtagccgc cgccgttgct tggcggtgtc gggggcatgg cgctgtggta
    13201 cagttgcgct tttgccgttt ttacgccccc gccatgtccc agcacgcttt ttcccccgac
    13261 aagcacacca cgctgtcccc tcggcagtgg tacgaagacg ccgcccaaaa acccggattc
    13321 atccgtgatg ccgctcaatc catggctatt gaccatctgg atgcgctgta tcaagaactg
    13381 ctggtgttca agaaaaagcg ccattcgttt ctgggtaaat ccttgcgcaa gcccagcatt
    13441 ccgcgcgggc tgtatttctg gggtggggtg gggcgcggca agagcttttt gatggatgcg
    13501 ttctacgcct gtgtgccgta tcggcgcaaa cggcggattc actttcacca tttcatgatg
    13561 gaagtgcata acgagctgaa aaccctgacc aacgccaccg acccgctgat caccgtggcc
    13621 gagcgcatcg cgcagaaaac ccggctgctg tgctttgacg agtttcatgt gtctgacatt
    13681 gctgacgcca tgattctggg ccgcttgatg aaagagctgt tcgagcgtgg ggtgatttgt
    13741 gtgctgacct ccaattaccc gccagacgag ctttacccca atggcttgca gcgcatcaac
    13801 tttttaccgg ctatcgagct gctgaaaaac tggctgaccg tgctgaatgt ggatggcggc
    13861 aatgactacc gcctgcgcga gctgacccgc gagcccttgt tcatcacccc gctgaaaggc
    13921 agccgcgaca agctggatgc gctgtacgag cgcttgtcgt ctggcaccgg caaagagctc
    13981 aagcacgaaa ttattgtgtt cgaccgtaaa atccgcgcca tccgccactc gccgggggcg
    14041 atctggtttg atttcatgga aatctgcggc ggcccgcgcg cgcagaccga ttatctggaa
    14101 atcgcccgcg aataccagac ggtgtttgtc tccaatgtgc ccaagctggc tccgcatcaa
    14161 tccagcgagg cgcgccgctt tacctggctg gtggatgtat tttatgacca ccgcgtcaaa
    14221 ctggtgctgt ctgccgaagt tgaggccgaa cagatttaca ccgatggcgt acagtccagt
    14281 gaattcttcc gcaccgccag ccggctgacc gaaatgcaat ccacctcgta tctgggcttg
    14341 ccgcacttgt ctgacgtgtt cgagcacacc ccagccgcca atctggccga gtgagcccac
    14401 ggccgccgca ggcgcggcgg ccgtgccgga tcaggctact ttggccagtt ctgcccgcat
    14461 cgcggcgatg gtggctgcat agtcatcagc gccatagatg gccgagcccg ccacaaaagt
    14521 gtcggcgcca gcggcggcga tttcggcgat attgtccacc ttcaccccac cgtccacctc
    14581 cagccagatg tcgcggccgg tggcctcgcg gtaggcgtcg atgcggcggc gcgcttcgcg
    14641 cagcttgttc agcgcctgcg gaataaagct ctggccgcca aagccggggt tgaccgacat
    14701 gatcaggatc atgtccagct tgtccatcac atggtccaga tggtgcagcg gggtggccgg
    14761 gttgaacacc aggccagcct tgcaaccaca gtcgcggatc agcgccagcg tgcgatcgac
    14821 atgctcggag ccttccgggt ggaaggtgat caagttggcg ccggccttgg cgaacatcgg
    14881 aaccaggctg tccaccggct tgaccatcaa atgcacatca atcggcgcct cggtcagtgg
    14941 gcggatggcc tcgcacacca gcgggccaat ggtgagattg ggcacgtagt ggttgtccat
    15001 cacgtcaaag tggatgatgt cggcgccggc ggcgatcaca tcggtgactt cctggcccag
    15061 acaggcgaaa ttggcggaaa gaatgctggg tgcaatacgg aaagtgcgca tggtgggtct
    15121 tgtaaacggg gaaagtgccc ggattttacc cgatagtgtc tgcttgccag cttggtcagc
    15181 ggaaaaaacc atgttggcat gaatttttgt tcagttactg acgatagact ataaagaaat
    15241 catcctgact ttgactgacg ctggccgcgc gccagtgagc gtcgttcacc aaaccttccc
    15301 ggccttgccg ggtatggcca cacgcccagc caagcgcgct gtgcagggtt tggtgagagt
    15361 ggggtgggcg tcggggctgt cccgcgccat ttgaaccgga tgtcccggcg cgccgggttt
    15421 gcaagtgaaa ggctgttttt gtcatgacct cccccattcg tgtggaaccg caggcgtact
    15481 acatcgccga ccaatccaac cccgccaacc actgctatgt gtttgcctac cgtatccaga
    15541 ttcacaacga cggcgatgtc ggcgtgcagc tgatctcgcg cagttggtac atcaccgacg
    15601 ccaacaacca tgtgcgcgaa gtgcacggcg aaggggtggt gggcgagcag ccgcgtctgc
    15661 aaccgggcga aacctacgaa tacgtcagcg gcgcggtgct ggccaccgcc cagggcacca
    15721 tgcgcggcag ttataatttc accgccgatg atggccgcga atttgaagcg ccagtgccgg
    15781 agttcttcct gaacggcgcg caagcgctgc actgatcgac attcccgacg gcggcggctg
    15841 cgggcgtcgc tattgggcga tgcccccgcg ccggccttgg ccggctttcc tgaccgcgcc
    15901 ccgacgggac tgccatgcga ttcacccatt ccgggcctgc gcccaccgag cgtaacgacg
    15961 ccaaaaccct gaaaaccctg ctgccctact tgctggaatt caagggccgg gtggcgttgg
    16021 cgctgtcttt gctgatgctg gccaagctgg ccaatgtcag cgtgccgtgg tttctcaagg
    16081 atattgtcga tcagctgtcg gccacccagg ccgcgttggc catgccgctg ggcctggtgg
    16141 cggcgtatgg cctggcgcgg ctggccacca gcgtgtttgg cgagctgcgc gacgcggtgt
    16201 tcgccaaggt cacccagcgc gccatccgcc gcattgcctt gcaagtgttc gagcacctgc
    16261 accggctgag cttgcgcttt catctggagc gccagaccgg cggcatgagc cgcgatatcg
    16321 agcgcggcac caagggcatt ggctttttgc tcaatttcac cctgttcaac atcctgccca
    16381 ccctggtgga aatcggcctg gtggcgggca ttctgctggt gaattatcac tggtcgttcg
    16441 ccgtggtgac ttttttgacc attgcgtctt atatcggctt caccctcggg gtgaccgaat
    16501 ggcgcatggt gtttcgccgc agcatgaacg agctggattc gcaggccaat agcaaggcga
    16561 ttgacgcgct gctcaattac gagacggtca agtattttgg caatgagcgc tacgaagtgg
    16621 cccgctacga cggcaatctg gcctcgtggg agcgctccgc ggtgcgcaac cagacctcgc
    16681 tgaattttct gaacgccggc cagggggtga tcatcgccgt gggcgtcacc gtgctggtcg
    16741 gcctggccgc ccgtggcgtg gtggatggca gcatgagcgt gggcgatatg gtgctggtca
    16801 acgcctattt gattcagctg tacgcgccgt tgaattttct gggctttgtc taccgcgaga
    16861 tcaaacactc gctggccgat atggagcgca tgttcaccct gctggagcaa ggcgaagaag
    16921 tggccgaccg ccccggcgcg caaaccctgg ccactcgcca ggcggccatc cgctttgagc
    16981 aggtgcagtt tggctacgac ggcaagcggc agattctgca cgacgtcagc tttgacatcc
    17041 cggccggcaa gacggtggcg gtggtggggg ccagcggcgc cggcaaatcc accctgtcgc
    17101 ggttgttgtt ccgtttttac gatgtgggcg ccgggcgcat caccctcaat ggcgtggaca
    17161 tccgcgagct gacccaggcc agcctgcgcg cgcatatcgg catcgtgccg caggacaccg
    17221 tgctgttcaa cgacagcatc tactacaata ttgcgtatgg acgccccgac gccagccgcg
    17281 aacaggtgat cgaagcggcg cgcgccgcgc atattcacga ttttgtcagc cagttgcccg
    17341 acggctacga caccgtggtg ggcgaacgtg ggctgaagct ctctggcggc gaaaagcagc
    17401 gggtggcgat tgcccgcacc ctgctgaaaa acccgccgat cctgatcttt gacgaggcca
    17461 ccagcgcgct ggattcgcac accgaaaaag ccatccaggc cgagctggcg gagattgccg
    17521 cccaacgcac caccctgatc atcgcccacc ggctgtccac cgtggtggac gcgcatcaga
    17581 ttgtggtgat ggaaggcggg cgcattattg aacaaggcag cttccgcgac ctgctcgacc
    17641 gtcagggccg ttttgcccag atgtggcagc tgcaattgca agacgccgac catgcctgaa
    17701 ccgactttgc cttccttgcc ccttgatttg ccgcccgccg cgctgtggcg gcactttgcc
    17761 acgctgtgcc ggctgccgcg cccctcgcgc cacgaaggcc aggtgatgga ccacctgtgc
    17821 cgctgggcca gcacgcgcag cctgaactgg acgcaagacg ccgccggcaa tcttttgatc
    17881 cgcaaacccg ccagccccgg catggccgaa cgggaaaccg tggtgctgca aggccatgtg
    17941 gacatggtgt gccagcagga cggcgacgcc catgattttt tcagcgaccc gattcagccc
    18001 tggctggccg acgatggctg ggtgcgcgcg cgcggcacca gcctgggcgc tgacaacggc
    18061 attggcgtgg ccgccatgct ggcggtgctg gaaagcgatg acatcgccca tccgccgctg
    18121 gaggcgctgt tcaccgtcaa cgaagaagcc ggcatggacg gcgctcatga tctggcggaa
    18181 gacctgctca ccggccgctt cttgctgaat ctggacaccg aagactgggg cgagttttac
    18241 attggctgcg ccggcggcga agacgtgatt ttgcgccgcc agtgcgagcg cgagccgctg
    18301 ccgcccgagc accacgcctg gcggctgacc gtgcgcggct tgtgcggcgg tcattccggg
    18361 gtggacatcc accgctaccg cgccaacgcc acccgcctgc tggcgcggct gttgtccgaa
    18421 ctggctgggc gctttggcct gcgcgtcggc caactggctg gcggcgcgct gcgcaatgtg
    18481 attccgcgcg aagccagcgc cacgctggcc gtgccggaaa gcaagctgga agcgctgcaa
    18541 cacaccttgc tggactggca aaagcgggtg gccgccaccc tgccggaatg ggaccacggc
    18601 ctgaccgtca cccttggccc ggcgcagctg aacgcggtgc tgtcgctgca cgaccaggcg
    18661 gtgctgattg acctgctcaa cgccctgccc gacggcatgg ccggcctgag ccccgccctg
    18721 cccgatgtgg tgcacacctc gtccaatctg ggcgtggtca gcctggacaa tggccgcttt
    18781 caggcggtgt cgatggtgcg ttcgctgagc gatgacgggc tggaaagccg ggtggcttct
    18841 ttattggcct gcggccgtct ggccggctgt tcgggcgtgc gcgagggttt ttaccccggc
    18901 tggaccccgc gcccggaatc gccgctgctg gccttggcca gccaagtgta tcagcgccag
    18961 cacggccagc gacccaaggt gaaggtgatt cacgccgggc tggaatgcgg gattttgctg
    19021 ggcaaatacc cggacctgga cattatttcg tttggcccgg acatccgtgg cgcgcactcg
    19081 ccgtccgagc gggtgaatgt ggcttcggtg gcgcgcttct gggattttct gtgcgagctg
    19141 ctggcgcaga ttcctgcgcg acggctggac tgagtcagat aaccggacaa gaaaaaaccc
    19201 gctgatattc accagcgggt tttttcttgt ccggcccacc gccgagggtg ggccaggttt
    19261 caggcttact gagcggcagc ttcgctcggc gccggggcgg cagcggcggt ttcttcgttc
    19321 agcacggcct tgatcgacag gcggatgcgg ccgcgctcgt cggtttccag caccttcacg
    19381 cgcacttgct ggccttcctt gaggtagtcg ccgacattgg cgatgcgctc attggcgatc
    19441 tggctgatgt gcaccagacc atcgcggccc ggcatgatct gcacgatggc gccgacattc
    19501 ttgtccagaa tcttcatcac cgtgccttcg tagatcttgc cgatttccac ttcggcagtg
    19561 atctgttcga tgcggcgctt ggcggcttcg gcgccttcgc cggacaccga agcgatggtg
    19621 atcaggccgt cttcgccgat gtcgatgctg gtgccggttt cttcggtgat cgaacggatc
    19681 accgagccac ccttgccgat cacgtcgcgg attttctccg gattgatctt catggtgtac
    19741 aggcgcgggg cgtgggcgga cagctcttgc ggggcgtcca cggcgttctt catcaggccg
    19801 aggatgtgca gacggccttc tttggcctga tccagcgcca cgcgcatgat ttccttggtg
    19861 atgccctgga tcttgatgtc catttgcagc gcggtcacgc cgttggcggt gccggccacc
    19921 ttgaagtcca tatcgcccag gtgatcttca tcgcccagga tgtcggtcag cacggcaaaa
    19981 cggttgcctt ccagaatcag gcccatggcg atgccggcca cgtggtcttt cagcgggacg
    20041 ccagcgttca gcatggccag gcagccgccg cacaccgaag ccatcgacga ggagccgttg
    20101 gattcggtga tttccgacac cacgcgcatc gagtagctga actcgtcagc cggcggcagc
    20161 gcggccacca gggcgcgctt ggccaggcgg ccgtggccga tttcgcggcg cttggggctg
    20221 ccgacgcggc cggtttcgcc ggtggagtac ggcgggaagt tgtagtgcag catgaagcgt
    20281 tcggtgtatt cgccagccag ggcgtcaatc acctgctcgt cctgcttggt gcccagcgtg
    20341 gtcaccacca gcgcctgggt ttcaccacgg gtgaacagcg ccgagccgtg ggcgcgcggc
    20401 agcacgccgg tgcggatgct gatcgggcgc acggtgcggg tgtcgcggcc gtcgatgcgc
    20461 ggctcgccgg ccaggatct
//
LOCUS       DAOPYX010000012        20477 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_2698_length_20477_cov_8.478259, whole genome shotgun sequence.
ACCESSION   DAOPYX010000012 DAOPYX010000000
VERSION     DAOPYX010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 20477)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20477)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20477
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2698_length_20477_cov_8.478259"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            365..1432
                     /gene="tsaD"
                     /locus_tag="PKN55_01335"
     CDS             365..1432
                     /gene="tsaD"
                     /locus_tag="PKN55_01335"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012697837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HNX69917.1"
                     /translation="MRVLGIESSCDETGVALYDTEAGLLAHALHTQMAMHAEYGGVVP
                     ELASRDHIRRVIPLTRECLKTAGLTLADLDAIAYTQGPGLGGALLVGAAVANGLAFGL
                     NLPVIPVHHLEGHLLSPMLADPAPTFPFVALLVSGGHTQLMAVRGVGDYETLGETLDD
                     AAGEAFDKTAKLLGLPYPGGPELSRLAEHGDPTRFTLPRPMLRSGDFDMSFSGLKTAV
                     LTLVRQQEAECGGALDDATRTDICRAFQEAIVEVLVSKSLAALKATGMQRLVIAGGVG
                     ANRQLRAALDRAAQRRRFAVYYPPLSLCTDNGAMIAYAGAARLALAQPAGVFSVRPRW
                     PLASLAPVSSSPRSTDSGLSS"
     gene            1429..3342
                     /locus_tag="PKN55_01340"
     CDS             1429..3342
                     /locus_tag="PKN55_01340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014086722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNX69918.1"
                     /translation="MIQLKNLSLRRGAKELFNSVTLTINPGQRVGIVGGNGAGKSSLF
                     ALLRGQLHADSGDALFPAGWTVAHVAQETPALSCSALDYVLDGDAELRALEQQLAAAE
                     TAHDGEAIGRLHSELAAIDAYAAPARAGKLLAGLGFSQEATTRPVASFSGGWRMRLNL
                     AQALMCRSDLLLLDEPTNHLDLEAVIWLEQWLASYPGTLLLISHDREFLDAITTQIIE
                     VARGQLTAYTGNYSDFEDQRAERLAQQQQAFDKQQRTIAHLESFITRFKAKATKAKQA
                     QSRIKALARLERVAAAHVDTPFDFHFEPPEHSPNPLLQVELADIGYGEAPILRRVSLS
                     VESGARIGLLGPNGAGKSTLVKLLAGELAPLNGQLKTARELKIGYFAQHQLDTLREDE
                     SPLWHLQKLAPTTREQELRNFLGGFNFIGDMATEPVGPLSGGEKSRLALALIVWQRPN
                     LLLLDEPTNHLDLDMRAALTLALQDFIGSLIVVSHDRALLESVTDQYWLIDAGQVQPF
                     DGDLADYRAYRLAQQAADAAPRASAAGGAEVDRKTQKRQEAEARQRLSEQKKPLLKRL
                     TALERELTPLNAEKAALDAQLADEAIYSAERKSEMQAALKRQGEVSARLAELEEDWLM
                     VQMEIEALEEANI"
     gene            complement(3389..4609)
                     /locus_tag="PKN55_01345"
     CDS             complement(3389..4609)
                     /locus_tag="PKN55_01345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012697767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HNX69919.1"
                     /translation="MTASAMLSVEAALEFLLSRARPLTETESVHLLNAGQRVLAGELR
                     ARLDVPPANNSAMDGYAVRSAEVHADAWLPVSQRIAAGDAPGPLAEGTVARIFTGAPI
                     PAGADAVIMQEQAHADGERVRFSAVAKTGQNIRLAGEDIARDGVVLPAGAQLGPAQLG
                     LAASVGEAYLDVLRPLRVAVLFTGDELAEPGETLTAGKIYNSNRYWLRGLLEALGCQV
                     RDLGIIPDSLAATRLALADAAATSDVVLTCGGVSVGEEDHVKHAVEREGQLDLWKIAI
                     KPGKPLAFGRIAHADFIGLPGNPVSGYVTFLLLIAPFIRARQGRAARASWPVSRLTAA
                     FDWPRKDPQRTEFVRVRKASRDGQTVLELFPKQGAGVLTSMAWADGLARIPANHIVKA
                     GEQIDYLPFDELLS"
     gene            complement(4606..5103)
                     /gene="mobB"
                     /locus_tag="PKN55_01350"
     CDS             complement(4606..5103)
                     /gene="mobB"
                     /locus_tag="PKN55_01350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012697769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HNX69920.1"
                     /translation="MKVLAFVGYSGSGKTTLLEKLIARLSADGVDLAVIKHTHHDVDP
                     DEIGRDSWRHRHAGARQVMLAAARRRMLIEELPKANDLPLAEHLAALRPCQLVLVEGF
                     KHERGLAKIEVFDPALSRPALYLTDPDVLAVACDMPLPDCPLPRFSRHDVEGILQLIH
                     KSVLS"
     gene            complement(5333..6289)
                     /locus_tag="PKN55_01355"
     CDS             complement(5333..6289)
                     /locus_tag="PKN55_01355"
                     /inference="COORDINATES: protein motif:HMM:NF017807.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNX69921.1"
                     /translation="MNFSLPHLGTGAARTPSSLLSFPRLAELPLGPLLAGLAALFWAG
                     NFVAGRALHDSLSPLQLAFGRWSVALLCLLPFAWPRLRAQAGALWAARWLILALALTG
                     VTACNTLIYQGLQSTPATQAVLLNAFTPVMVLIYAVCSGQRQPARGQWLGLVLSLVGV
                     LVLVSQGAPWRLSALTPSGGDAWVFAGCVCWAAYTVLMRRLPTGLDRLALTAACMLLG
                     WLLLAPLALWDISQHGLPAVSSAVLAGVLYLGVFPSVVAYLCYNRAIAELGAERASAC
                     LHLVPAFGALLSCLLLGETLHPWHVLGIAAVFAGLALGRSSR"
     gene            6815..7654
                     /locus_tag="PKN55_01360"
     CDS             6815..7654
                     /locus_tag="PKN55_01360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX69922.1"
                     /translation="MKIALLITVETARGMHTALPRVLDALHAAQASATVCLHLGRDAG
                     APLTGRRRERARWYGWGSLWRGRVWPGARLDKLAPAALKAIGHAGCVAGLHLEASRVW
                     RGTLAAQPLAARVAQFRRLAGLAAEAPFTVNLPGGLASWPLLRQMQALGVGALTGVPG
                     QHGFLPCHHAELLAVPVLPTSLPNLGDVLRAERGQADAAVHTLLSHSAGLAGPALCWL
                     DAERIGGVWQAEFARLLAGWREQGHVLCAVSDSLLTHAVLPHAELEISPRLALRQGTT
                     RFA"
     gene            complement(7781..8440)
                     /locus_tag="PKN55_01365"
     CDS             complement(7781..8440)
                     /locus_tag="PKN55_01365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012697508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000041 - transition metal ion transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HNX69923.1"
                     /translation="MNFPAQFFAPATLWLCHALCAALLAQAVWRVRWRQLDPVGLNAW
                     MGACVVVMLFWSLKGGFKPGLEFHLLGGAAFALMAGPWLALIGLAIVLAGVTAYGMAE
                     WAAFGVNFLVMAAAPVAVTALTLRLAQRLPANYFVYIFFNAFLTGWLSFFCAGLLGVS
                     VLALADAYAPDYLFGDALSFYFLLSWSEAFTTGLMLAIFVVYKPHWVATFDDRRYLLR
                     K"
     gene            8632..9477
                     /gene="nadC"
                     /locus_tag="PKN55_01370"
     CDS             8632..9477
                     /gene="nadC"
                     /locus_tag="PKN55_01370"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012697304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HNX69924.1"
                     /translation="MSAVLPPSHLIAQHVSRALAEDIGPRDWTAELIPANAIGRATVV
                     AREAAVIAGQAWFTECFRQVDPRCHVVWRVQEGEMVFADTELCTLDGPARALLTAERS
                     ALNFLQTLSSTATLTRRYADAIAGYRARIMDTRKTLPGLRVAQKYAVTVGGGVNQRVG
                     LYDGILIKENHILAAGGIRPALEAAFAVAPSNVSVQIEVENLQELAEALDAGARLVLL
                     DNMSLTDMRAAVQMAAGCAELEASGNVSLNTIAAIAATGVDRISIGKLTKDISAVDLS
                     MRFAL"
     gene            complement(9681..>10580)
                     /locus_tag="PKN55_01375"
     CDS             complement(9681..>10580)
                     /locus_tag="PKN55_01375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018749618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HNX69925.1"
                     /translation="RLAARPMQLLAMCTRPFVMLLTFSTHTLLRLLGVRQHTPNGVTE
                     EEIHAMLEEGSETGVIEQHQHEMVRNVFRLDDRQLGSLMIPRSDVVFIDINLPPETSL
                     NQLIESEHSRFPVCDGSLDKVLGVIHAKQALAYVAKGELPDYLSHLQAGVYVPETLTG
                     MELLEQFRANNMQMAFVIDEYGDTKGIVTLQDVLEAVTGEFTPRNADDAWAVRREDGS
                     WLLDGAIPIPEMKDRLELKTTPEEDKGRYHTLSGMMMLLLGRVPSTGDHADWEGWRFE
                     VVDMDEKRIDKVLAAPLRNTETA"
     assembly_gap    10581..10680
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(11110..12462)
                     /locus_tag="PKN55_01380"
     CDS             complement(11110..12462)
                     /locus_tag="PKN55_01380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012698014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNX69926.1"
                     /translation="MTFAELGLSPEVLKAVEELGYASPTPIQAKAIPVVLGGSDVLAA
                     AQTGTGKTAAFTLPLLSRLRAHANNSASPAMHPVRALILTPTRELADQVSASVAQYNK
                     YLPLRSAVVFGGVAMDPQKEALRRGVEVLVATPGRLLDHIEQKNVMLNRVEMLILDEA
                     DRMLDMGFVQDIRRIMSLLPANRQTLLFSATFAPEIKRLAADFMREPELVEVARQNAT
                     SENIEQVVYAVDAARKRHLLVHLLQARSIAQVIVFCRTRQGAEQLSRELKRDGLAAEA
                     IHGDKAQSQRLETLSAFKDGQVRVLVATDVAARGLDIEELPFVVNFELPNSPEDYVHR
                     IGRTGRAGSKGVALSLMGEDEQRQLEAIETLIKQKLPPQTERRFWPSWMPRPEREREV
                     AASAPVDTVSSKRALAALSAAPTRSTPSTPAPLRRFSHAALRGEPREIPALLLPPRYR
                     "
     gene            complement(12561..13481)
                     /locus_tag="PKN55_01385"
     CDS             complement(12561..13481)
                     /locus_tag="PKN55_01385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018150204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CobD/CbiB family protein"
                     /protein_id="HNX69927.1"
                     /translation="MTLLSIIVALALEQLRPLGNRNRLFLLFTRYANTLERNLNAGQN
                     RHGVLAWLAGVLPVCLAVYGVYALCLQVSPFLALAWNVLVLYLTMGFRHFSSALTEIT
                     DALAEQRVMDARAALARWSGQSTSELEVNEIARVTIEQGVIDSYRHVFATMFWFAVLP
                     GPVGAVLYRFSSILFQKWGMRDSQFGDLFGRFAYRAQETLDWLPTRLTALSFAIMGDF
                     EDAVYCWRAQAQAWGHYAYGILLASAAGALGIKLGDPLRQDYTVKFRPELGVGDDADP
                     RYLKSAVGLVWRVVMFWLFMIAVISLAWWV"
     gene            complement(13582..14049)
                     /locus_tag="PKN55_01390"
     CDS             complement(13582..14049)
                     /locus_tag="PKN55_01390"
                     /inference="COORDINATES: protein motif:HMM:NF016985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlgN"
                     /protein_id="HNX69928.1"
                     /translation="MSDRLQELNRLIAEERAGYTELITLLEQEQRELISGTAHKLEAL
                     AQQKAIVLQELDALAQQRGDHMRVLGMVSLNAWRAWLADKPELLPAWLGLEVLAQKAK
                     LINEINGKLILTHMEQVEASLEVLFTSDQSTFAYSRDGGARSALIGGRRLGSA"
     gene            complement(14066..14368)
                     /gene="flgM"
                     /locus_tag="PKN55_01395"
     CDS             complement(14066..14368)
                     /gene="flgM"
                     /locus_tag="PKN55_01395"
                     /inference="COORDINATES: protein motif:HMM:TIGR03824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HNX69929.1"
                     /translation="MKIDSTGRPVVTRPVGGKGRASARSSVTGGESRSEDNVDINPLA
                     SQMAAAEQEIRERPAFDAEKVAAIKDAIAAGKFQINPEAIADSLIAVSRELLQNKS"
     gene            complement(14500..15171)
                     /gene="flgA"
                     /locus_tag="PKN55_01400"
     CDS             complement(14500..15171)
                     /gene="flgA"
                     /locus_tag="PKN55_01400"
                     /inference="COORDINATES: protein motif:HMM:TIGR03170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HNX69930.1"
                     /translation="MKTILAVASLCACTAASAAGYQDMAGLIGQARQFAEASLAGTQA
                     RVTVGVVDTRLRLPACPIPPMPNWQGGRFEGNTAVEMSCPQLGWNIRVPVKVERETMV
                     VAMRRPVTAGSMLTPDDVMLVPARGNMASVSVTALNEAIGQVVGQNLPAGLILRRGLL
                     RQQQVIQSGQKVQLMATDGSFQVSAEGVALKNGSVGEVIGVRMPSGRVISGVVSASGA
                     VEVRF"
     gene            complement(15213..18542)
                     /locus_tag="PKN55_01405"
     CDS             complement(15213..18542)
                     /locus_tag="PKN55_01405"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HNX69931.1"
                     /translation="MPLWLLVMVVLVLYWAQLLQAHRDQLAQVEAQTRLRATQMASAL
                     SLQIGSLFNGVDYVSRTLANTYRDDQPEVFRQALDLAKKKAFPEGALVQIAVADAQGL
                     VQFSSLQDKSDGKPVSIADREHFSVHAQKLVEGMYISHPVQGRVSKRWSIQFSRAIYH
                     NQVFAGVVVLSISPDYISNFFREVFYQSGDVVMLLRDDGSYLARSQQQESVLGKAVQP
                     PGSGFLIDPGQQRGEYLTKAPLDNVMRYYAWHRAASYPVVVNVGLNQDTALQAVQHAL
                     QLSLLRNGLGTLMILLGMSGISWLFFQKQRGMAQLQESEERLGLALQGGDLGSWDWDI
                     PSDAISFSVRWAQMLGYQPDDLPHTLNGLQTLTHPDDWPRKLAALRNHLEGASVVFES
                     EHRMLHRDGHWVWILAKGRVTHWSSAGAPLRAVGTHLDITARKQAEAARAELQARLSK
                     LVAQVPGMVYQYRLRPDSTACFPYASVGIRAIYQLQPEDVRNNADPVFAVIHPDDLQR
                     VMTSIRVSAVNLTPWQCEYRVRFANGVVRWLSGQANPEREADGGTLWHGYIHDITATY
                     TVQQALRQSEERLRLTVSAVRDGLWEWDTRSGVIHWDNRCYDMLGYEPQAFPLTLMVW
                     TEMLHPLDREHTLAKLDPVSMPREGARVECRLRTAQHDWRWVEIRGQLVQDASGQHVR
                     MMGTYSDISQRVEQAHLRRALLDQSAAAIFLAAPDRTIRHANARAVEMFAKDGKTLRN
                     LSFSQLHVDAQTYQAFGSYYDTLRRNGQVRLEHPLRDAHGQIRWFDTHGTLLDPDRPD
                     ADVIWTLIDTTERHQAETALAAERVRLTAIIDRFPAGVWVDDEDGHIVLINQNLCDLL
                     EVNDVPTHLVGQVQRQLLSSLPESSRALFETDGRSRSDGYLAREIPANHGRTLEVDQQ
                     PITHDEQYFGRLWLVRDITGQKRREAILETLAATDALTRLPNRRAFIAHLEQYAQDLV
                     RGTQQSGAVLMIDLDFFKRINDSYGHGVGDKVLQQTASVIRHTLRADDVAGRLGGEEF
                     AVLLPGAPADHACEVAERLRAAIADVRVEVNEGLVHVTASIGVHAIADATLSSDDWLA
                     RADSALYRAKHEGRNRVVRDQ"
     gene            complement(18810..19400)
                     /locus_tag="PKN55_01410"
     CDS             complement(18810..19400)
                     /locus_tag="PKN55_01410"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025033.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNX69932.1"
                     /translation="MSWDERYSTAEYVYGTEPNGFLVEHAGLLRGPVLSLAEGEGRNG
                     VFLAGQGLTVHGVDSSAVGLAKAQALAAARGVSLHVEQADLAEFVPACAAYGAVVSIF
                     AHVPGAVRTRLYPLLVDCLQPGGLLLLEAYSLAQLPRTTGGPKDPDLLMSVDAIRCGF
                     AGLEPVLLQELTREVHEGRLHTGLASVVQFIGRKPC"
     gene            19790..>20477
                     /locus_tag="PKN55_01415"
     CDS             19790..>20477
                     /locus_tag="PKN55_01415"
                     /inference="COORDINATES: protein motif:HMM:NF025091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/phosphoenolpyruvate mutase
                     family protein"
                     /protein_id="HNX69933.1"
                     /translation="MPADATHQARDFLALHQRGTFLMPNAWDAGSAKYLAACGFAALG
                     TTSAGIAFAAGHRDSDPRQSVDAMFAAIASIVAAVSIPVSADLEAGFGDSLDDVQRTF
                     TRAIAIGCAGGSLEDVADYATPGQFVLRSQQDAIERVRAARAAIDQHATPFVLTARTE
                     CFLTGHPSPLAEAIDRLCAFRDAGADCLFAPGISQPADIATLVREVNAPLNVLTAGPA
                     AQLTLAQLRDL"
BASE COUNT         3960 a         6707 c         6276 g         3434 t
ORIGIN      
        1 agtcagggag gtttccggcc cggctaagcg gcggcgcagc cgacgcgcca ttccgtggcg
       61 gccctggccg gcgagaagcg caaggcaccc gaagccgcag gcgtagggcg cgacgcccgg
      121 gtcgcctttc tttcccctat ttctttggcg agacaaagaa atagggtcgg gtgcggggcg
      181 aagccctgcc caaaatcagc ggccagcagc gctgcgcgcc agcgcagcca tccgccaggc
      241 ggcagccttc catcaccaaa gccccacagc ataccgcttc ccctcccgcc cccaaagcac
      301 ctcttgtccc atccatatgc cggaaccgct acaataccgg attgccttag cgacgaccca
      361 ccccatgcga gttctgggaa ttgaatcctc ctgcgacgaa accggcgtcg ccctctacga
      421 caccgaagcc ggcctgctgg cccacgccct gcacacgcaa atggccatgc acgccgaata
      481 cggcggcgtg gtgcccgagc tggccagccg cgaccacatc cgccgggtga ttccactcac
      541 ccgcgaatgc ctgaaaaccg ccgggctcac cctggccgac ctggacgcca tcgcctacac
      601 ccaaggcccc ggcctgggcg gcgcgctgct ggtcggcgcg gcggtggcca acggcctggc
      661 ctttggcctg aacctgccgg tgattccggt gcaccacctg gaaggccatc tgctgtcacc
      721 gatgctggcc gacccggcgc cgacgtttcc ctttgtggcg ctgttggtgt ctggcggcca
      781 cacccagctg atggcggtgc gcggcgtggg cgactacgaa accctgggcg aaaccctgga
      841 cgacgccgcc ggcgaggcct tcgacaaaac cgccaagctg ctgggcctgc cctaccccgg
      901 cgggccggag ctatcgcgcc tggccgaaca cggcgacccc acacgcttta ccctgccgcg
      961 gccgatgctg cgctcgggcg actttgacat gagtttttcc gggctgaaaa ccgccgtgct
     1021 gaccctggtg cgccagcaag aagccgagtg cggcggcgcg ctggacgacg ccacccgcac
     1081 cgacatctgc cgcgcctttc aggaggcgat tgttgaggtg ctggtgagca aatcgctggc
     1141 cgcgctcaag gccactggca tgcaaaggct ggtgattgcc ggcggcgtgg gcgccaatcg
     1201 ccagctgcgc gccgcgctgg accgcgccgc ccagcgccgc cgctttgccg tgtactaccc
     1261 gccgctgtcg ctgtgcaccg acaacggcgc gatgatcgcc tatgccggcg cggcgcggct
     1321 ggcgctggcc cagccggccg gcgtattttc ggtgcgtccg cgctggccgc tggccagcct
     1381 ggccccggtg tcttcttccc cccgttcgac cgattctggc ctgagttcat gattcaactg
     1441 aaaaacctgt ccctgcgccg tggggccaag gagctgttca acagcgtcac cctcaccatc
     1501 aaccccggcc agcgcgtcgg cattgtcggc ggcaatggcg cgggcaaatc cagcctgttt
     1561 gccctgttgc gcgggcaatt gcacgccgac agcggcgatg cgctgtttcc ggccggctgg
     1621 acggtggcgc atgtcgccca ggaaaccccg gcgctgagct gctcggcgct ggattatgtg
     1681 ctggatggcg acgccgagct gcgcgcgctg gagcaacaac tggccgccgc cgaaaccgcc
     1741 cacgatggcg aggcgattgg ccgcctgcac agcgaactgg ccgccatcga cgcctacgcc
     1801 gccccggcgc gcgccggcaa gctcttggcc ggcctgggct ttagccagga agccaccacc
     1861 cgaccggtgg ccagcttctc tggcggctgg cgcatgcgcc tgaacctggc gcaggcgctg
     1921 atgtgccgct cggatttgct gctgctggat gaaccgacca accacctgga cctggaagcc
     1981 gtcatctggc tggagcaatg gctggccagc tatccgggca ccctgctgct gatttcgcac
     2041 gaccgcgagt ttctggacgc catcaccacc cagatcatcg aagtggcgcg cggccagctg
     2101 accgcctaca ccggcaatta cagcgacttt gaagaccagc gcgccgagcg cctggcccag
     2161 cagcaacagg cattcgacaa gcaacagcgc accatcgccc acctggaaag ctttatcacc
     2221 cgcttcaagg ccaaggccac caaggccaag caggcgcaaa gccgcatcaa ggcgctggcg
     2281 cggctggagc gggtggcggc ggcgcatgtg gacacgccgt ttgattttca cttcgagccg
     2341 cccgaacaca gccccaaccc gctgctgcaa gtcgagctgg ccgacatcgg ctatggcgaa
     2401 gcgccgattc tgcgccgggt gagcctgtcg gtggaatccg gcgcgcgcat cggcctattg
     2461 ggccccaacg gcgccggcaa atccaccctg gtcaagctgc tggctggcga gctggcgccg
     2521 ctgaacggcc agctgaaaac cgcgcgcgag ctgaaaatcg gctactttgc ccagcaccag
     2581 ctggacaccc tgcgcgagga cgaatcgccg ctgtggcatc tgcaaaaact ggcgccgacc
     2641 acgcgtgagc aagagctgcg caattttctg ggcggattca attttattgg tgacatggcc
     2701 accgagccgg tgggcccgct gtccggcggc gaaaaatccc gcctggcgct ggcgctgatt
     2761 gtctggcagc ggcccaatct gctgctgctg gatgaaccca ccaaccatct ggacctggac
     2821 atgcgcgcgg cgctgaccct ggcgctgcaa gactttatcg gcagcctgat tgtggtgtcg
     2881 cacgaccgcg ccctgctgga aagcgtcacc gaccaatact ggctgatcga cgccggccag
     2941 gtgcagccgt ttgacggcga cctggccgac taccgcgcct atcgcctggc ccagcaagcc
     3001 gccgacgccg cgccgcgcgc cagcgccgca ggcggcgcgg aagtggaccg caaaacgcaa
     3061 aagcgccagg aggccgaagc ccgccagcgc ctgtccgagc agaaaaaacc gctgctcaag
     3121 cgcttgaccg cgctggagcg cgagctcacc ccgctgaacg cagaaaaagc cgcgctggac
     3181 gcgcaactgg ccgacgaggc gatttacagc gccgagcgca aaagcgaaat gcaggccgcg
     3241 ctcaagcgtc agggcgaagt cagcgcccgg ctggccgagc tggaagaaga ctggctgatg
     3301 gtgcagatgg aaatcgaagc actggaagaa gccaatatct gatgatcagg gcgctgggct
     3361 gcatgccaac gcccttgcgg ccgggttttc agctcagcaa ttcatcaaat ggcagatagt
     3421 caatctgctc gccggccttg acgatgtgat tggccggaat gcgcgccaga ccgtcggccc
     3481 aggccatcga ggtcagcacg ccagcgcctt gtttggggaa cagctccagc acggtctggc
     3541 cgtcgcggct ggccttgcgc acacgcacaa attcggtgcg ttgcgggtct ttgcgcggcc
     3601 agtcaaacgc tgcagtcaga cggctgaccg gccagctggc gcgggctgct cggccctggc
     3661 gagcgcggat aaacggcgca atcagcagca agaaggtgac atagcccgac accgggttgc
     3721 ccggcaggcc aatgaaatcc gcatgggcaa tccggccaaa ggccagcggc ttgcccggtt
     3781 tgatggcgat tttccacagg tccagctggc cttcgcgctc gacggcgtgc ttgacgtggt
     3841 cttcttcgcc caccgacacc ccgccgcagg tcagcaccac atcgctggtg gcggcagcgt
     3901 cagccagcgc cagacgggtg gcggccaggc tgtcaggaat aatgcccagg tcgcgcacct
     3961 ggcagcccag cgcttccagc aggccgcgca gccagtagcg gttggaattg tagattttac
     4021 ccgccgtcaa cgtctcgccc ggttcggcca gttcgtcgcc agtgaacaac accgccaccc
     4081 gcagcgggcg caacacatcc agataggctt cgcccaccga ggccgccagg cccagttgcg
     4141 ccggccccag ctgggcgccc gccggcagca ccacgccatc cctggcgata tcttcgcccg
     4201 ccaggcggat gttctggccg gttttggcca cggcgctgaa acgcacgcgt tcgccatcgg
     4261 cgtgggcttg ctcctgcatg atcaccgcat ccgcgccagc cggaatcggc gcgccggtaa
     4321 agatgcgcgc cacggtgcct tcggccagcg ggccaggggc atcaccggcg gcaatgcgtt
     4381 gcgacaccgg cagccaggca tcggcgtgaa cctcggcgct gcgcacggcg tagccatcca
     4441 tggcgctgtt attggcgggc ggcacgtcca ggcgggcgcg cagctcgccg gccaacacgc
     4501 gttggccggc gttgagcagg tgaacggatt cggtttcggt cagcgggcgg gcgcggctca
     4561 gcaaaaactc cagcgcggcc tcgacgctca gcatggcaga tgcagtcatg acaaaacact
     4621 cttgtgtatc aattggagga tgccttccac gtcatggcgg gaaaagcgcg gtagcggaca
     4681 gtccggcagc ggcatatcgc acgccacggc cagcacatca gggtcggtca aataaagcgc
     4741 cgggcgcgac agggccggat caaacacttc gatcttggcc agcccacgct cgtgcttgaa
     4801 gccttccacc agcaccagct gacacgggcg cagggcggcc aggtgctcgg ccagcggcag
     4861 atcattggcc ttaggcagct cttcaatcag catgcgccgc cgcgcggcag ccagcatcac
     4921 ctggcgcgcg ccagcgtggc gatgccgcca actgtcgcgc ccgatttcgt cggggtcaac
     4981 atcgtggtgg gtgtgcttga tcaccgccag atcaacgcca tcggcgctca ggcgggcaat
     5041 gagtttttcc agcagcgtgg tcttgccgct gccggaatag ccgacaaagg ccaggacttt
     5101 catcagggct tccatggatt gggtggtgca ccgtgctgaa catgagttca gcatgcccgg
     5161 tcacggtgca tccgtcattg acctgcatca taccgcagaa gcatggaggt tttcgaactt
     5221 ggcgtggcgc acaacatccc gcgcaagcgc ccaggacaga tgtgcggccg tagaccatac
     5281 aaacagtaca gccagaccgc gcaagcacgc caagcagttt tttgagccac gctcagcgcg
     5341 acgaacgccc cagcgccagg ccagcaaaca ccgccgcaat gcccagcaca tgccaggggt
     5401 gcagggtttc acccagcaac aggcaggaca atagcgcgcc aaaggccggc accagatgca
     5461 ggcaggcgct ggcacgctcg gcgcccagct cggcaatcgc ccggttgtag cacagatagg
     5521 ccaccaccga tgggaatacc cccagataca gcacgccggc cagcaccgcc gacgacacgg
     5581 caggcaaacc atgctggctg atatcccaca acgccaacgg cgccagcagc aaccagccca
     5641 gcagcatgca agcagcggtg agcgccagcc gatccagccc ggtgggcagg cggcgcatca
     5701 gcacggtgta ggccgcccag cagacgcagc cggcgaacac ccaggcatcg ccgccgctgg
     5761 gggtaagcgc cgacagccgc cacggcgcgc cctggctgac caacaccagc acccccacca
     5821 aggacaagac cagacccagc cattggccgc gcgctggctg gcgctggccg ctgcacacgg
     5881 cgtagatcaa caccatcacc ggggtgaagg cgttcagcag caccgcctgg gtggccgggg
     5941 tggattgcaa gccttggtaa atcagtgtgt tgcaggccgt cacgccggtc agcgccagcg
     6001 ccagaatcaa ccagcgcgcc gcccacaaag ccccagcctg agcgcgcagg cgcggccagg
     6061 caaacggcag caggcacagc agcgccacgc tccagcggcc aaacgccagt tgcaaggggg
     6121 agaggctatc gtgcagggcg cggccagcga caaaattgcc cgcccagaac aaagcagcca
     6181 agccggccag caaaggcccc agcggcagtt cggccaaacg cgggaaagac agcaaagaag
     6241 aaggcgtgcg ggcagcgccc gtgcccagat gaggcagaga gaagttcatg gcagatttct
     6301 tttcgggtag aaaagcacac acggccatgc gcttgtggca catggtcaga ccagccggtg
     6361 cgcaagatga gcgcaccagc acactgcgca agcagagcca atgccgggtg gggcgtgtct
     6421 ctgtgcgcac gattcaggca agcaagcttg atgcccgccc ggcgggcagt gtgccatgaa
     6481 cacgccgggc cggccaaacc gtccggcctg atgcgcacga ctcaccgcac gcagtgtgag
     6541 agcgcgcaaa cacgggaaag ccacgtcacc atgcggggag cgtttgctga acaatactga
     6601 ccgggagtgc gcaccgccat cgacataaga gccgctgaca aaatacgcct ggcgtcgtga
     6661 cacggccttg ccgcacggct tgtactgtct gtggcggcgc gcccagccag agacgcttcg
     6721 ctgagtttgg ttaacggctc tcgcgcactg ctggtcaaag ccgccggcat ggcggacaat
     6781 tcgcgcccat tgtcgttctg tgatgaagat tgccgtgaag attgccctgc tgattaccgt
     6841 tgaaaccgcg cgcggcatgc acaccgcgct gccgcgtgtg ttggacgcgc tgcatgccgc
     6901 ccaggccagc gccacggtgt gcctgcatct gggccgtgat gccggtgcgc cactgactgg
     6961 ccgccggcgt gagcgggcgc gctggtatgg atggggcagc ttgtggcgcg gccgggtctg
     7021 gccgggggca cggctggata agctggcgcc agcggcgctg aaggcaattg gccatgccgg
     7081 ctgtgtggcg ggattgcacc tggaagcatc tcgagtgtgg cgcggcaccc tggccgctca
     7141 gccgctggcg gcgcgagtgg cgcagtttcg ccgtctggcc ggtctggccg ccgaggcgcc
     7201 ctttaccgtc aacttgccgg gcggcctggc cagttggccg ctgctgcggc agatgcaggc
     7261 gcttggcgtg ggcgcgctca ctggcgtgcc cggccagcac gggtttttgc cttgccatca
     7321 tgccgagcta ttggccgtgc cggtattgcc caccagcttg cccaatctgg gtgatgtgct
     7381 gcgcgccgag cgcggccagg cagatgccgc cgtgcacacg ctgctgagtc atagcgccgg
     7441 gctggctggg cctgcgctgt gttggctgga tgccgagcgg attggcggcg tctggcaagc
     7501 ggagtttgcc cggctgctgg ccggctggcg cgagcaaggg catgtgctgt gcgcggtcag
     7561 cgatagcctg ctgacgcatg ccgtgctgcc gcacgccgag ctggagatca gcccgcgcct
     7621 tgcgctcaga caaggcacga ctcgctttgc ttaagacggc gctgagctga acagtcatta
     7681 tttttgcgct gactgaaatt cagtgcgttg atttttctgg attttgtgtg tgggagagac
     7741 gattttctga atcagcgcct ccctgagcgc ccaagcaacc ctacttgcgc aacaaatagc
     7801 gccggtcgtc aaacgtggcc acccagtgtg gcttatacac cacgaaaatc gccagcatca
     7861 ggccagtggt gaacgcctcg gaccaggaca gcaaaaagta aaagctcagc gcgtcgccaa
     7921 acagatagtc cggcgcatag gcgtcggcca gcgccagcac gctgacgcca agcaggccgg
     7981 cgcaaaaaaa gcttagccag ccggtcagga aggcgttgaa aaaaatatac acaaagtaat
     8041 tggccggcag ccgctgcgcc aggcgcaggg tgagggcggt caccgccacc ggcgcggcgg
     8101 ccatcaccaa aaaattgacg ccaaacgccg cccattcggc catgccgtaa gccgtcacgc
     8161 cagccagcac aatcgccagg ccaatcagcg ccagccatgg cccggccatc agcgcaaacg
     8221 ccgcgccgcc cagcaggtga aactccagcc ccggcttgaa gccgcccttg agcgaccaga
     8281 acagcatcac gaccacacag gcgcccatcc aggcgttgag cccgaccggg tccagctgtc
     8341 gccagcgcac ccgccacacg gcttgcgcca acagggcggc gcacaaggca tggcacagcc
     8401 acagcgtggc aggcgcaaaa aactgggcgg gaaaattcat ggcaaaggtc cggcggcaaa
     8461 gactttattc gtcatgatag caaaagccgg gcaggccgtt gtgcggcgca atgtcgcggc
     8521 gggcaatcct gctttgacac gccgccccgg cgcccgcaca atcgaaagtt tggcgcgctg
     8581 ccaggctgca cagcaggcag gcgcgatggt tttttactca ggatgctggc catgagcgct
     8641 gtcttgcccc cttctcatct gattgcccaa cacgtctccc gcgccctggc cgaggacatc
     8701 ggcccgcgcg actggacggc cgagctgatt cccgccaacg ccattggccg cgccactgtg
     8761 gtagcgcgcg aggcggcggt gattgccggg caggcgtggt ttaccgagtg ttttcgtcag
     8821 gtggatccgc gctgccatgt ggtgtggcgg gtgcaagaag gcgagatggt gtttgccgac
     8881 accgagctgt gcacgctgga cggcccggcg cgcgcactgc tgaccgccga gcgctcggcg
     8941 ctgaactttt tgcaaacctt gtcttccacc gccacgctga cccgccgcta cgccgacgcc
     9001 atcgccggct accgggcgcg catcatggac acccgcaaaa ccctgcccgg cctgcgcgtg
     9061 gcgcaaaaat acgcggtgac ggtgggcggc ggcgtcaatc agcgcgtcgg gctgtacgac
     9121 gggattctga tcaaggaaaa ccacatcctg gccgctggcg gcatccgccc ggcgctggaa
     9181 gccgcgtttg ccgtggcgcc gagcaatgtc agcgtgcaaa tcgaagtgga aaacctgcaa
     9241 gagctggccg aagcgctgga cgcgggcgcg cgcctggtgc tgctggacaa tatgtcgctg
     9301 accgatatgc gcgcggcagt gcaaatggcc gccggctgcg ccgagctgga agcctcgggc
     9361 aatgtcagcc tgaacaccat cgccgcgatt gccgccaccg gggtggaccg gatttcgatt
     9421 ggcaagctga ccaaggatat ttcggcggtg gatttgtcga tgcgttttgc gctgtaaacc
     9481 gtccagcagc agcacttcat gacaaacccc cgtccgcttt ctggcggacg ggggtttgca
     9541 cggcaaaggc cacaagaacg cgccatcgcg cctggagggt ttgctgagcg gcgcttacac
     9601 aggggtgttt gcgatcacga gcaggatatt gtgacgtctg agcctgtctg cgccattcag
     9661 catccgaatg gcgctggggc tcaagcggtt tcggtattgc gcagcggcgc cgccaacacc
     9721 ttgtcgatgc gtttttcgtc catgtccacc acttcaaagc gccagccttc ccaatcggca
     9781 tggtcgccag tgctgggcac gcggccaagc aacagcatca tcatgccgct gagggtgtgg
     9841 taacggcctt tgtcttcttc cggggtggtt ttcagctcca ggcggtcttt catctccgga
     9901 atcgggatgg cgccgtccag cagccaggag ccatcctcgc ggcgcaccgc ccaggcgtcg
     9961 tcggcgttgc gcggggtgaa ttcaccggtc accgcttcca gcacgtcttg cagggtgaca
    10021 atgcccttgg tgtcgccgta ttcgtcgatc acaaaagcca tttgcatatt attggcgcga
    10081 aactgctcca gcagctccat gccggtcagg gtttccggca catagacacc cgcttgcaga
    10141 tggctcaggt agtctggcag ctcgcccttg gcgacatagg ccagcgcctg tttggcgtga
    10201 atcaccccca gcaccttgtc caggctgccg tcgcacaccg gaaaacgcga atgctcggat
    10261 tcaatcagct ggttcaggct ggtttctggc ggcaggttga tatcgataaa caccacatcc
    10321 gagcgcggaa tcatcagcga acccagctgg cggtcgtcca ggcggaacac attgcgcacc
    10381 atttcgtgct ggtgttgttc gatcaccccg gtttccgagc cttcttccag catggcatga
    10441 atttcttctt cggtgacgcc gttcggggtg tgctggcgca cgccgagcag gcgcagcagg
    10501 gtgtgggtgg agaaagtcag cagcatgaca aacggccggg tgcacatggc cagcaactgc
    10561 atcggccgcg ccgccagccg nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10681 tcggcggcca gtttcatcag ccgcgccttg cgcgcggtaa ccaaggcaat ctctgacatg
    10741 gcgaacaggc cattgatcac caccaggcca atcagcatca gcacttccac agcacagccc
    10801 cgcattcagg agaagcaaaa cctatagctt gccggaaagc gcgtgacagg cacaagcaca
    10861 gcggggcgtc agcggcgggg ctcattgagc gggtgaaaca cggtcgggga tagtccatat
    10921 gcagtcgggg caagcccgaa gttgtctcca gaaacaaaaa gatgttggcc gcctatcaga
    10981 ttggccagct tgagcgcggc ggtgtgaacg gcgatttatc cgcgcctgtt cgagccacac
    11041 catgtcataa agttcagacg cggcaacgcc cctggcgggc ggcaggggcg ttgacgtggc
    11101 gcaacaagct tagcggtagc gcggcggcag cagcagcgcc gggatttcgc gcggctcgcc
    11161 gcgcagggcg gcatggctga agcggcgcag tggcgccggg gtggatggcg tgctgcgggt
    11221 gggcgccgcc gacagcgcgg ccagggcgcg cttgctgctg acggtgtcca ccggcgcgct
    11281 ggcggccact tcgcgctcgc gctcggggcg cggcatccag ctgggccaga agcggcgttc
    11341 ggtttgcggc ggcagctttt gcttgatcag cgtttcgatg gcttccagct ggcgttgctc
    11401 gtcctcgccc atcagcgaca gcgccacgcc cttgctgccg gcacggccgg tgcggccaat
    11461 gcggtgcacg tagtcttctg gcgagttggg cagctcgaaa ttcaccacaa acggcagttc
    11521 ttcgatgtcc aggccacggg cggccacgtc ggtggccacc agcacgcgca cctggccatc
    11581 cttgaacgcc gacagggttt ccagccgctg gctttgcgcc ttgtcgccgt gaatggcttc
    11641 ggcggccagg ccgtcgcgct tgagttcgcg cgagagctgc tcggcgccct ggcgggtgcg
    11701 gcagaacaca atcacctggg cgatgctgcg cgcttgcagc agatgcacca gcaaatggcg
    11761 cttgcgcgcg gcgtccaccg cgtacaccac ttgttcgatg ttttcgctgg tggcgttttg
    11821 ccgggccact tccaccagct ccggctcgcg catgaaatca gccgccaggc gcttgatttc
    11881 cggggcgaag gtggccgaga acagcagggt ttgccgattg gccggcagca ggctcatgat
    11941 gcggcggatg tcctgcacaa agcccatgtc cagcatgcgg tcggcttcgt ccaggatcag
    12001 catttccacc cggttgagca tcacgttttt ttgctcgatg tggtccagca gacggcccgg
    12061 cgtggccacc agcacttcca cgccacggcg cagcgcttct ttttgcggat ccatcgccac
    12121 gccgccaaac accaccgccg aacgtagcgg caggtatttg ttgtactgcg ccacgctggc
    12181 gctgacctgg tcggccagtt cgcgggtggg ggtgaggatc agcgcgcgca ccgggtgcat
    12241 ggcgggcgag gcgctgttgt tggcgtgcgc gcgcaggcgc gacagcagcg gcagggtgaa
    12301 ggcggcggtc ttgccggtgc cggtctgggc ggcggccagc acatcgctgc cgcccaacac
    12361 caccggaatg gccttggctt gaatcggcgt cggtgacgca tagcccagct cttcaaccgc
    12421 cttgagcact tctggcgaca agccaagctc ggcaaacgtc atgacggggt tcctttatga
    12481 ctaaacacag attgcaatac tcacgctgtt cgtcagactg aacccacgaa cagcgcagaa
    12541 aaaaacagag ggaaaagcgc ttacacccac caggccagcg agatgacggc aatcatgaac
    12601 agccagaaca tcaccacccg ccagaccagg cccacggcgc ttttcaggta gcgcgggtcg
    12661 gcgtcgtcgc ccacgcccag ttccgggcgg aacttgacgg tgtagtcctg gcgcagcggg
    12721 tcgcccagct tgatgcccaa ggcgccggcg gcgctggcca gcaggatgcc gtaagcgtag
    12781 tggccccagg cttgcgcctg agcgcgccag caatacacgg cgtcttcaaa gtcgcccatg
    12841 atggcaaagc tcagcgcggt cagccgggtg ggcaaccagt ccagggtttc ttgcgcacgg
    12901 taggcaaagc ggccaaacaa atcgccaaac tggctgtcgc gcatgcccca cttttgaaaa
    12961 agaatgctgg agaaacggta cagcacagcg cccaccgggc cgggcagcac ggcaaaccag
    13021 aacatggtgg cgaacacatg gcggtagctg tcgatcacgc cttgctcgat ggtgacgcgg
    13081 gcgatttcgt tgacttccag ctcactggtg gattggccag accagcgcgc cagcgcggcg
    13141 cgggcgtcca tcacccgttg ttcggccagg gcgtcggtga tttcggtgag cgcgctgctg
    13201 aaatggcgaa agcccatggt caggtagagc accagcacat tccaggccag cgccagaaac
    13261 ggcgacacct gcaggcacag cgcatacacg ccgtacacgg caaggcagac cggcaacacg
    13321 ccagccagcc aggccagtac gccatggcgg ttctggccgg cgttgaggtt gcgctccagg
    13381 gtgttggcgt agcgggtgaa caacaggaac aggcggttac ggttgcccag cgggcgaagc
    13441 tgttccagcg ccagggcaac aataatcgaa agcagggtca tgcgaacatc cggggcacag
    13501 agtcaagtgc cagcacgata ccataaagcc cggcagctgg gcagcctgcc cgggcgggta
    13561 tggcaagatg ggcaataaaa atcaggcaga gccgaggcgg cggccgccaa tcagtgccga
    13621 gcgcgcacca ccgtcacggc tataggcaaa ggtgctctga tcactggtga acagcacttc
    13681 caacgaggct tccacctgct ccatgtgggt caaaatcagc ttgccattga tctcgttgat
    13741 cagcttggct ttttgcgcca gcacttccag tcccagccag gccggcaaca attcaggctt
    13801 gtcagccaac caggcgcgcc aggcattcaa agacaccatg cccagtacgc gcatgtggtc
    13861 gccacgctgt tgggccagtg cgtccagctc ttgcaggaca atggctttct gctgggccag
    13921 ggcttccagc ttgtgtgcgg tgccggagat caactcgcgt tgttcttgct ccagcagggt
    13981 gatcagttca gtgtagcccg cgcgttcttc cgcaatcagg cgattcagtt cctgcagacg
    14041 atcactcatg gattcctgtc tcacatcaag atttgttttg cagcagttcc cggctcaccg
    14101 caatcaggct gtcggcgatg gcttcaggat tgatctggaa tttgccagcg gcaatggcgt
    14161 ccttgatggc agccactttt tcggcgtcaa atgccgggcg ctcgcggatc tcctgctcag
    14221 ccgccgccat ctggctggcc agcggattga tgtcaacgtt gtcttccgaa cggctttctc
    14281 cgccggtgac cgacgaacga gcgctggcac ggcccttgcc gccaactggc cgggtgacaa
    14341 ccggtctgcc cgtggagtcg attttcatga tggcatcact tcgtgcataa ggaattcaag
    14401 cttcggtatc ggtaaaacgc tggcaatctt gagtatttta gccggttttt acccgcttgg
    14461 cgcacaactt taccggactg aacgcaccag cagcacgctt cagaaacgca cttccaccgc
    14521 gccactggcg ctgaccacgc cactgatcac ccggccggaa ggcatacgca caccgatcac
    14581 ctcacccacg ctgccatttt tcaatgcgac accttcggcc gagacctgaa aactgccatc
    14641 ggtcgccatc aactggactt tctggccaga ctgaatcacc tgctgttggc gcaacaatcc
    14701 ccgacgcagg atcagaccag cgggcagatt ctggccaacc acctggccga tggcttcatt
    14761 gagcgctgtc accgacaccg aggccatatt gccacgagcg ggcaccagca tcacatcatc
    14821 cggggtcagc atgctgcccg cggtgactgg ccgacgcatg gccaccacca tggtttcccg
    14881 ctcgaccttg actggcacac gaatattcca gcccagttgc gggcaagaca tttccacggc
    14941 ggtgttgccc tcaaaacggc cgccttgcca gttgggcatg gggggaatcg ggcaggcagg
    15001 caggcgcagg cgggtgtcca ccacgccaac agtcacgcgg gcctgggtgc ccgccagaga
    15061 agcttcggca aactgccggg cctggccaat caggccggcc atatcctgat agccggcggc
    15121 gcttgccgcc gtgcatgcac acagcgacgc cactgcaaga atcgttttca tgctggcgat
    15181 tctacccggt ttttgtccgg acgtcggcct gttcactgat cgcgcaccac gcggttgcgt
    15241 ccttcatgct tggctcgata cagcgcactg tctgccctcg ccagccagtc gtcgctagag
    15301 agcgtggcat cagcgattgc atgcacgcca atactggccg tgacgtgcac caggccttcg
    15361 ttcacctcca cccgcacatc agcgatggca gcacgcaggc gctcggccac ttcacaggca
    15421 tgatcggccg gggcgccagg caagagcacg gcaaattctt cgccaccgag acgaccggcc
    15481 acatcatcgg cgcgcagggt gtgacgaatg accgaggcgg tttgttgcag caccttgtcg
    15541 cccacgccgt ggccatagct atcattgata cgcttgaaaa aatccagatc aatcatcagc
    15601 accgcaccgc tttgctgcgt gccacggaca agatcctgag cgtattgctc cagatgcgca
    15661 ataaaggcgc gacgattggg taaccgggtc agcgcatcgg tagccgccag ggtttccaga
    15721 atggcttcgc ggcgcttttg cccggtgatg tcacgcacca gccacagccg gccaaaatat
    15781 tgctcgtcgt gggtaatcgg ctgctggtcc acttccagcg tgcgcccatg gttggcaggg
    15841 atttctcgcg ccagataccc atccgagcgc gagcgcccgt ccgtttcaaa caaggcccgg
    15901 ctgctttctg gcagactgct cagcaactgg cgctgcacct gccccaccag atgcgtcggc
    15961 acatcgttga cttccagcag gtcgcacaga ttctggttga tcagcacaat atggccgtct
    16021 tcatcatcca cccacacacc ggcagggaaa cgatcaatga tggcagtgag ccgtacacgt
    16081 tcggcggcca gggcagtttc ggcctgatga cgctcggtcg tgtcgatcag cgtccagatc
    16141 acatcggcat cgggacgatc cggatccagc agggtgccat gggtgtcaaa ccagcggatc
    16201 tggccgtgcg cgtcacgcag cgggtgttcc agccgcacct ggccattgcg gcgcagggtg
    16261 tcgtaatagc tgccaaatgc ttggtaggtt tgtgcatcca catgtagctg gctgaacgac
    16321 agattgcgta atgtcttgcc atccttggca aacatttcca cggcgcgagc attggcgtgg
    16381 cggatggtgc ggtctggggc ggccaggaag atggccgccg cgctttggtc cagcagcgca
    16441 cggcgcaggt gtgcctgctc gacgcgctgg ctgatgtcgc tgtacgtgcc catcatgcgc
    16501 acatgctggc cactggcgtc ctgcaccagt tggccacgga tttccaccca gcgccagtcg
    16561 tgctgggccg tgcgcagccg gcactccacc cgagccccct cccgcggcat cgacaccgga
    16621 tcaagtttgg ccagggtgtg ctcacgatcc agcggatgca gcatttcggt ccacaccatc
    16681 agggtcagcg gaaaagcctg tggctcgtag cccagcatgt cgtagcagcg gttatcccag
    16741 tggatcactc cactgcgggt atcccattcc cacaggccat cgcgcacggc cgacaccgtc
    16801 agccgcaggc gctcttcgct ctggcgcaac gcctgctgca cggtgtaggt ggcagtgatg
    16861 tcatggatat agccatgcca caaggtgccg ccatcggctt cgcgctcggg gttggcctgg
    16921 ccgctgagcc agcgcaccac gccattggcg aagcgcacac ggtattcgca ctgccacggt
    16981 gtgagattga cagccgatac ccggatggaa gtcatcaccc gttgcaggtc atccggatga
    17041 atcacggcaa acaccggatc agcgttattg cgcacgtctt ccggctgcaa ttgataaatg
    17101 gcacgaatgc ccacactggc atacggaaag caggctgtgc tatccgggcg tagccggtat
    17161 tggtacacca tacctggaac ctgagccacc agcttgctca gacgcgcctg caattcggca
    17221 cgggcggctt cggcctgctt gcgggcagtg atgtccagat gagtgcccac tgcgcgcaag
    17281 ggcgcgccgg ccgaactcca gtgggtgaca cgccccttgg ccagaatcca tacccagtga
    17341 ccatcacggt gcagcatgcg gtgttcggac tcaaacacca cgctggcgcc ttccagatgg
    17401 tttctcagtg cggccagctt gcgcggccag tcatccggat gggtcagtgt ctgcagacca
    17461 ttaagggtgt gtggcagatc atccggctga taacccagca tctgcgccca acgaacacta
    17521 aagctaatcg catcgctggg aatatcccag tcccaggagc ccagatctcc accctgcagg
    17581 gccagaccca ggcgctcttc actttcctgc aattgcgcca tgccgcgttg cttctggaag
    17641 aacagccaac tgatgccgga catccccagc agaatcatca gcgtgcccag gccattgcgc
    17701 agcaggctca gctgcagggc gtgctgaaca gcctgcaggg ccgtatcctg gttcaggccg
    17761 acgttgacca ccacgggata actggcagca cgatgccagg cgtagtaacg catgacatta
    17821 tccagaggcg ctttggtcag atattcgcca cgctgctggc cagggtcaat cagaaaaccc
    17881 gagccggggg gttgcacagc tttgcccagc acgctttcct gctgctggga acgggccaga
    17941 tagctgccat catcgcgcag cagcatgacc acatccccgg actgataaaa cacctcacga
    18001 aagaaattcg agatgtaatc cggcgagata gacagcacaa ccacaccggc aaatacctgg
    18061 ttgtggtaaa tggcgcgcga aaactggatg gaccagcgtt ttgatacccg gccctgcaca
    18121 ggatggctga tgtacatgcc ctccaccaac ttttgtgcat gcacgctaaa atgctcgcga
    18181 tcggcaatcg acaccggttt gccgtcggat ttatcctgca agctggaaaa ctgcactaaa
    18241 ccctgggcat cggcgacggc aatctgcacc aatgcgccct ccgggaaagc tttctttttg
    18301 gccagatcca gcgcctgccg gaagacttcc ggctgatcat cccgataagt gttcgccagc
    18361 gtgcgcgaga cataatccac gccattgaac aacgaaccga tctgcaacga caaagcgctg
    18421 gccatctgcg ttgcacgcaa acgggtctgg gcttctacct gagccagctg atctcgatgg
    18481 gcttgcaaca gttgcgccca atacagcacc agcaccacca tcaccagcag ccataacggc
    18541 aaggcgcgac gtatcagggc agaataactg ggtatggagg cggaagcggg ggctttggtg
    18601 tgtgtgggca taactcttca tcaccgcggc tggaccatgc ccgtggcggg cacgacaggc
    18661 tgcgctttgg ctaccctgcg ggaaaaacgg ttgacaagct tgtccggcat gttgccgaaa
    18721 acggcaacat gccggacaag ggctgctttt gtcattttat gtggctgaca ttgaattgtg
    18781 caagctgaaa gcacgtgatc agtcacgtct cagcatggct tgcgaccaat gaattgcacg
    18841 actgaggcca ggccggtatg caggcgcccc tcgtgcacct cacgcgtcaa ctcttgcagc
    18901 agcacaggct ccagcccggc aaagccgcac cggatggcgt ctaccgacat cagtaaatcc
    18961 gggtcttttg gcccgccggt tgtgcgtggc agctgcgcca gcgaatacgc ctccagcagc
    19021 agcaacccac ctggctgcag acaatccacc agcaacgggt ataaccgcgt acgcactgcg
    19081 ccaggcacgt gggcaaaaat tgacaccacg gcgccatacg ccgcgcaggc gggcacaaac
    19141 tcagccagat ccgcctgctc gacatgcagg ctgaccccac gcgcagcggc cagcgcctga
    19201 gccttggcca agcccaccgc cgaactgtct accccatgaa ccgtcaagcc ctgcccagcc
    19261 agaaacacac cattgcgtcc ctcgccctcg gccagtgaca acaccgggcc acgcagcagg
    19321 ccggcatgct caaccaaaaa cccgtttggt tcagtgccat agacatattc agcggtcgaa
    19381 tagcgctcgt cccagctcat cgtacaggct ccgtaaatac cataaagccc aataaaaaca
    19441 caaaagcggc caagacgata gcggtgtaca cgttcacgcc atcgtcaata ataacagagt
    19501 catctatcgc cccttggcac acctgccgca ccgcacagcc aacacgcggc agcagcatgc
    19561 cagcgcaact cggcattaag agcggctcac aaaactcagc gaagcgatcc tggccaggcg
    19621 cacgactgca gacagtacgg gaagtacggc aaggtcgggc aaccacgcca ggagggtttt
    19681 gttagatgac aagcacatct gcgctcaccc tctcctctgc tggggatggc cagcccgccg
    19741 ccgcgcaggc atcatcccta tccgcgcctc acccgccaga aagctgctca tgcccgccga
    19801 cgctacccac caagcccgtg attttcttgc cctgcaccaa agaggcacct tcctgatgcc
    19861 caacgcctgg gacgccggca gcgccaaata cctggccgcc tgcggttttg ccgcactggg
    19921 caccaccagc gccggcattg cctttgccgc cggccatcga gactccgacc cgcgccaatc
    19981 ggtggacgcc atgtttgccg ccatcgccag cattgtcgcc gccgtgtcca tcccggtcag
    20041 cgccgacctg gaagccggct ttggcgacag cctggacgac gtgcaacgca ccttcacccg
    20101 cgccatcgcc atcggctgcg ccggcggcag cctggaagac gtggccgact acgccacccc
    20161 cggccaattt gtgctgcgct cgcagcaaga cgccatcgag cgcgtgcgcg ccgcccgcgc
    20221 cgccatcgac caacacgcta ccccctttgt gctgaccgcc cgcaccgaat gctttttaac
    20281 cggccacccc agcccgctgg ccgaagccat cgaccggcta tgcgcctttc gcgacgctgg
    20341 cgccgactgc ctgtttgccc ccggcatcag ccagccagcc gacatcgcca ccctggtgcg
    20401 cgaagtcaac gccccgctca acgtgctgac cgccggcccc gccgcgcaac tgaccctggc
    20461 gcaactgcgc gacctgg
//
LOCUS       DAOPYX010000013        20428 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_2710_length_20428_cov_10.817062, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000013 DAOPYX010000000
VERSION     DAOPYX010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 20428)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20428)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20428
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2710_length_20428_cov_10.817062"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(71..832)
                     /gene="pstB"
                     /locus_tag="PKN55_01420"
     CDS             complement(71..832)
                     /gene="pstB"
                     /locus_tag="PKN55_01420"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012696006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HNX69934.1"
                     /translation="MTDSIKLQVKNLNFYYGAFHALKNINMNIPAGKVTAFIGPSGCG
                     KSTLLRIFNRMFELYPNLRAEGELLLDGKNILGRDIDVNLLRAKVGMVFQKPTPFPMS
                     IYDNIAFGIKLYERLPKSEMDDRIEWALKKAALWEEVKNKLKQSGHSLSGGQQQRLCI
                     ARAVANRPEVLLLDEPTSALDPISTAQIEELVHELKENYTIAIVTHNMQQAARVSDYT
                     AYMYLGDLIEFGETDAIFTAPKQKATEDYITGKFG"
     gene            complement(845..1693)
                     /gene="pstA"
                     /locus_tag="PKN55_01425"
     CDS             complement(845..1693)
                     /gene="pstA"
                     /locus_tag="PKN55_01425"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008953456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HNX69935.1"
                     /translation="MDNALYRRRRLVNSFNKTMSFLTVAFGLFWLVWILYTLLKLGLG
                     GLSLDVFTQITPPPGSQGGLANAIYGSLLMTLFGTLIGTPVGILAGVYLAEFGNRGWL
                     APTTRFINDILLSAPSIVIGLFIYEVYVVSVGHFSGWAGSLALSLLVIPVVVRTTENM
                     LRLVPNSLREAAAALGAPQWKVIMSVTLRASKAGVITGILLAVARISGETAPLLFTAL
                     NNQFWNSNMNAPMANLPIVIFQFAMSPYEDWHTLAWAGSLLITFSVLTLNILARWFGS
                     QQTSSH"
     gene            complement(1721..2683)
                     /gene="pstC"
                     /locus_tag="PKN55_01430"
     CDS             complement(1721..2683)
                     /gene="pstC"
                     /locus_tag="PKN55_01430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008953455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HNX69936.1"
                     /translation="MSTQLSSSTQKPRTNLDPLFRLITRCFAFLVLALLAAILLSLIQ
                     GATPAIKHFGLGFLTNASWDPVAQEFGALVPIFGTLITATIALIIGVPVSFGIAMFLT
                     ELCPLQLRRPLGVAIELLAGIPSIIYGMWGLFVFAPWFADHVQPWMMETLGAIPGIGL
                     LFQGLPMGIGVFTAGLILGIMVIPFIASVMRDVFEVVPPMLKESAYGLGATTWEVVRH
                     VVLPYTKTGVVGGIMLGLGRALGETMAVTFVIGNAYQISAGLFNPSNSIASSLANEFA
                     EAEGELYLSALIELGLILFFITFVVLACSKLLLLRLKAGEGKES"
     gene            complement(2786..3826)
                     /gene="pstS"
                     /locus_tag="PKN55_01435"
     CDS             complement(2786..3826)
                     /gene="pstS"
                     /locus_tag="PKN55_01435"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008953454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HNX69937.1"
                     /translation="MKSTIRLVAAMTLSSAMFSGYAAAADITGAGATFPYPLYAKWAE
                     TYKAKSGVGMNYQSIGSGGGIKQIQAKTVDFGASDKPLTLDELNKAGLTQFPAVMGGV
                     VPVVNLPGVAPGQLKVTGPLLADIYLGKVTKWNDPAITSINPGVNLPAQNITVVRRSD
                     GSGTTFIWTNYLSRVSEEWKTKVGENSAVNWPTGVGGKGNEGVANYVTRIKGAIGYVE
                     YAYAKQNKIAHAAVKNQAGSFVQPDDLTFKAAAANASWKAADGFYQILTNQPGKDSWP
                     ITGATFILMHKKADKPEQSLESLKFFDWAYSHGDKLASDMDYVPMPDNVKKVIRDSWK
                     QITDTSGKPVWK"
     gene            4039..4800
                     /gene="tpiA"
                     /locus_tag="PKN55_01440"
     CDS             4039..4800
                     /gene="tpiA"
                     /locus_tag="PKN55_01440"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012696010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HNX69938.1"
                     /translation="MLNVATGTKWVLGNWKMNGRLASNQALLAALLTDAQVNVAHVGL
                     AVPTVYMAQVAQALSGQPMAWGGQAVSRFAADGAYTGEVSAAMLADLGASFTLVGHSE
                     RRQYFAESDEVLSAQICHAREAGLLPVVCVGEGLAAREAGEHEAVVAAQIDGVIAALQ
                     GSERVVVAYEPVWAIGTGLTASLGQIVAMHEKIRACLLEKLASSCTILVLYGGSVKAD
                     NAADILALSEVDGVLVGGASLVAEQFSKVCYGAAK"
     gene            4808..5155
                     /gene="secG"
                     /locus_tag="PKN55_01445"
     CDS             4808..5155
                     /gene="secG"
                     /locus_tag="PKN55_01445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011134495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HNX69939.1"
                     /translation="MEILKTLVWIVNMAAALAVIVLVLMQHGKGADMGAAFGSGSSGS
                     LFGASGSANFLSRTTAVCAAIFFSTCLALVFLSTPTPSNTLGVMSGQVETVAPASPAA
                     QPNGQNKPGQIPD"
     gene            5166..5250
                     /locus_tag="PKN55_01450"
     tRNA            5166..5250
                     /locus_tag="PKN55_01450"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5200..5202,aa:Leu,seq:gag)
     gene            5332..5688
                     /locus_tag="PKN55_01455"
     CDS             5332..5688
                     /locus_tag="PKN55_01455"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HNX69940.1"
                     /translation="MLENYFPIMLFVIIGLLVGVGPMLIGFLLGPRRPDPEKLSPYEC
                     GFEAFEDARMKFDVRYYLVAILFILFDLEIAFLFPWAVVLKEIGMFGFVAMLVFLAIL
                     VVGFIYEWKKGALEWE"
     gene            5679..6155
                     /locus_tag="PKN55_01460"
     CDS             5679..6155
                     /locus_tag="PKN55_01460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HNX69941.1"
                     /translation="MGVEGILEKGFVTTTADKVINWTRTGSLWPMTFGLACCAVEMMQ
                     AGASRYDLDRFGVVFRPSPRQSDLMIVAGTLCNKMAPALRKVYDQMAEPRWVLSMGSC
                     ANGGGYYHYSYSVVRGCDRVVPVDVYVPGCPPTAEALLYGIIQLQNKIKRTYTIAR"
     gene            6167..6760
                     /locus_tag="PKN55_01465"
     CDS             6167..6760
                     /locus_tag="PKN55_01465"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012696014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HNX69942.1"
                     /translation="MASTLEALKSAAEAALGANILSATIDRHELTLVCRADTIIETCT
                     LLRDTPSLRFEQCVDLCGMDYSAYRDGVWEGSRFAVVCHLLSVANNQRVRVRFFATDD
                     DFPVVPSLVDVWPGVNWFEREAFDLYGIIFDGHPDLRRLLTDYGFVGHPFRKDFPLSG
                     HVEMRYDPVQARVIYQPVTIEPRENTPRIIREENYGG"
     gene            6753..8006
                     /locus_tag="PKN55_01470"
     CDS             6753..8006
                     /locus_tag="PKN55_01470"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003798845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HNX69943.1"
                     /translation="MAEIRNYTLNFGPQHPAAHGVLRLILELDGEVVQRADPHVGLLH
                     RGTEKLAEYKTFLQSVPYMDRLDYVSMMCNEHAYVMAIEKLVGVEVPIRAQYIRVMFD
                     EITRILNHLLWIGAHALDIGAMTMFLYAFREREDLMDVYEAVSGARMHAAYYRPGGVY
                     RDLPDAMPQYKVSKIKNASQLAKLNENRQGSVLDFIEDFANRFPTYVDEYETLLTDNR
                     IWKQRTVGIGVVTPERALALGFTGPMLRGSGIAWDLRKKQPYEVYDRVDFDIPVGVNG
                     DCYDRYLVRVEEMRQANRIIKQCVEWLKKNPGPVITDNHKVAPPSRVNMKSNMEELIH
                     HFKLFSEGMHVPEGEAYAAVEHPKGEFGIYLVSDGANKPYRLKIRAPGYAHLSGLDEM
                     TRGHMIADVVSIIGTQDIVFGEIDR"
     gene            8006..8479
                     /gene="nuoE"
                     /locus_tag="PKN55_01475"
     CDS             8006..8479
                     /gene="nuoE"
                     /locus_tag="PKN55_01475"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HNX69944.1"
                     /translation="MLSPDALKQIDREVAKYPADQARSAVMGALRIAQTELGWLSTET
                     IEFVANYLGIPPVAAYEVATFYNMYDLKPVGKYKITVCTNLPCALGGGVDCADYLKHK
                     LGIGFGETTPDGKFTLVEGECMGACGDAPVLLVNNHSMCSFMSHEAIDKKLAELK"
     gene            8481..9776
                     /gene="nuoF"
                     /locus_tag="PKN55_01480"
     CDS             8481..9776
                     /gene="nuoF"
                     /locus_tag="PKN55_01480"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003759417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HNX69945.1"
                     /translation="MAVFTQGVIFEGVDTSDPNCWTLDAYVKRGGYEALKRILQQGIS
                     QDDVIAEVKGSSLRGRGGAGFPTGLKWSFMPRNFPGDKYLVCNTDEGEPGTFKDRDIL
                     RFNPHSLIEGMIIAGYAMGVKRAYNYIHGEIFEDYVLFERALDQARAAGFLGENILGT
                     SFTFDLFAHHGYGAYICGEETALLESLEGKKGQPRFKPPFPASFGLYGKPTTINNTES
                     FASVPFIIRDGGQKFLEAGKPNNGGTKLFSVSGHVNRPGNYEIPLGTPFATLLEMAGG
                     MKNGKKLKAVIPGGSSAPVLPGDIMMDCTMDYDSISKAGSMLGSGAVIVMNEDICMVK
                     ALERLAYFYHEESCGQCTPCREGTGWLYRVIHRIEHGQGRPDDLDLLTSVGNNMAGRT
                     ICALADAAVFPVRSFTKHFRHEFEYHIEHKKCLVEHKWC"
     gene            9813..12152
                     /gene="nuoG"
                     /locus_tag="PKN55_01485"
     CDS             9813..12152
                     /gene="nuoG"
                     /locus_tag="PKN55_01485"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008953446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HNX69946.1"
                     /translation="MLEIEIDGKKLTVPTGSTVMDAAHSNGTYIPHFCYHKKLSIAAN
                     CRMCLVEVEKAPKPLPACATPVTDGMKVHTHSDTAVKAQQGVMEFLLINHPLDCPICD
                     QGGECQLQDLAVGYGGVSSRYQEEKRAVPAKEMGPLVSAAEMSRCIHCTRCVRFTEEI
                     GGYQEIGMAHRGEHSQILPFLNKTVDSEISGNVIDLCPVGALTSKPFRYSARPWELSR
                     RKTVSPHDGLGANMIVQVKHNRVMRALPLENEAINECWLADRDRFSYEALSSDARLTR
                     PMVKQGGQWIETEWQAALEYIVKGLRGVTRDHGADAIGFLATPHSTVEELYLAKKLAG
                     ALGVESTDYRTRHADFSRDAAQQGALWLGSSIVELAAANALLVVGSVLRKEQPLLAAR
                     LRQSVKKGMALSVISPIQENLLSHVHARLTVSPVAMLDSLKAVLKAVLARKADASSTL
                     DVVSITPCAQAEAIADSLVNNDNAVIALGNLAQHSPHFGDLLTLANELARLTGGRVAV
                     LGEAANSVGAELTFKPSGKGANAGQMFSVPKKAYVLLNTELDCDSHNPHRALKAVEQA
                     EMVVAMSAFRSDAALQYADVMLPIAPFTETSGAFVNMEGLLQAFNGVVKPLGDARPAW
                     KVLRVLGNMLGVPGFEFESAEQVRAEWLAGLGGELKSKLNNGLSSLTLATPSSASGLV
                     RVGEVPMYQADALCRRAPSLQATADARAPRASAHSSVLAAAGVQSGQEVSLKQEGAAI
                     RIAIDADDSLPLGVVRLAAAHPATVALGGMFNSIELAQG"
     gene            12156..13202
                     /gene="nuoH"
                     /locus_tag="PKN55_01490"
     CDS             12156..13202
                     /gene="nuoH"
                     /locus_tag="PKN55_01490"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012696019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HNX69947.1"
                     /translation="MEFFQNLLGADAGLAVWTVLKIVAIVGPMMGAVAYLTLAERKVI
                     GYMQIRIGPNRVGPKGLLQPIADGIKLMLKEIVLPAKANKTLFLVGPILSIAPALAAW
                     AVVPFMPKLVLANIDASLLYIMAVTSIGVYGVIVAGWASNSKYAFLGALRSAAQIVSY
                     EIAMGFALVGVLMLSGSLNLVEIVNQQGQGIAGGSVFSWNWLPLFPMFLVYLISGVAE
                     TNRAPFDVAEGESEIVAGFHVEYSGMAFAVFFLAEYANMILIASMTALMFLGGWLSPL
                     PASVPVLGESGFHWLAIKAAFILFCFLWFRATFPRYRYDQIMRLGWKVFIPVTLVWLV
                     VVGFWMMSPYTLWK"
     gene            13219..13707
                     /gene="nuoI"
                     /locus_tag="PKN55_01495"
     CDS             13219..13707
                     /gene="nuoI"
                     /locus_tag="PKN55_01495"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008953444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HNX69948.1"
                     /translation="METIRNFFKTFLLVELVKGLALTGRHFFQRKITVQFPEEKTPQS
                     PRFRGLHAQRRYPNGEERCIACKLCEAVCPAMAITIESEQRDDGTRRTSRYEIDLTKC
                     IFCGFCEESCPVDSIVETHILEYHGEKRGDLYYTKQMLLAVGDQYEAEIAKNRELDAK
                     YR"
     gene            13717..14364
                     /locus_tag="PKN55_01500"
     CDS             13717..14364
                     /locus_tag="PKN55_01500"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017509622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HNX69949.1"
                     /translation="MSFQAFVFYVLSAILVFAGVRVITAKNPVHAALFLVLAFFTASG
                     HWLLLQSEFLAISLVLVYVGAVMVLFLFVVMMLDIDFEQLREGFWRNLPVAGTVAAIM
                     VAEMVLILTSPESNLNGFKEPAALAADYSNVKDLGRQIYTTYLYPFELAAVLLLLAIV
                     AAIALTMRKRKDTKWVDPAEQVKVRRNDRVRMIQMAAEPRVEDRGGDTNNDNAAA"
     gene            14400..14705
                     /gene="nuoK"
                     /locus_tag="PKN55_01505"
     CDS             14400..14705
                     /gene="nuoK"
                     /locus_tag="PKN55_01505"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HNX69950.1"
                     /translation="MLTLTHYLVLAAILFAISVLGIFLNRKNVIVLLMAIELMLLAVN
                     YNFIAFSHYLSDTAGQIFVFFILTVAAAESAIGLAILVVLFRNLRTINVEDLGSLKG"
     gene            14744..16762
                     /gene="nuoL"
                     /locus_tag="PKN55_01510"
     CDS             14744..16762
                     /gene="nuoL"
                     /locus_tag="PKN55_01510"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014085966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HNX69951.1"
                     /translation="MEMKSLYLLIALAPLAGSAIAGLLGWAIGRVAAHLVTIGGVAVS
                     AVGAVYVLNGLLNGAPTFDGPVYTWLTVGGIDYSVGFLIDNLTAMMMVVVTSVSLMVH
                     IYTIGYMHEDPGYQRFFSYISLFTFSMLMLVMSNNFIQLFFGWEAVGLVSYLLIGFWF
                     KKPTAIFANLKAFLVNRVGDFGFLLGIGMVLAYFGGSLHYADVFAAAPGLATKTVQIF
                     PGVEWSLMTVTCILLFIGAMGKSAQFPLHVWLPDSMEGPTPISALIHAATMVTAGIFM
                     VSRMSPLFELSDTALSFILVIGSITALFMGFLGIIQNDIKRVVAYSTLSQLGYMTVAL
                     GVSAYPVAVFHLMTHAFFKALLFLGAGSVIMGMHHEQDMRRMGGLWKYMPVTWFTSLL
                     GSLALIGTPFFAGFYSKDSIIEAVAASHIPGSSFAYFAVVAGVFITAFYSFRMYFLVF
                     HGEERFRHVHHDDHHGHHDDHGHGHAHVPHESPWVVTLPLVLLAIPSVIIGFIAIEPM
                     LFGDFFKNVIFINHEAHPVMEELAHEFHGAAGMATHALSTLPFWLALGGVATAWLFYM
                     KAPQIPAMFKAKLGAIHTLLENKYYLDDIYFGVFAKGSRGLGTLLWKVGDQLLIDGLL
                     VNGSAKVVGAFSAVVRKLQTGFIYSYATAMIIGVLGLMTIWFSGLIAH"
     gene            16805..18301
                     /locus_tag="PKN55_01515"
     CDS             16805..18301
                     /locus_tag="PKN55_01515"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008953440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HNX69952.1"
                     /translation="MVENLLSLAIWTPIVAGLLVLATGGDKNAGLARWVALLGSLAGF
                     VVSIPLFTGFQSLNGGMQFVEKAPWIASLNIHYYLGVDGISMLFVVLNSFTTVMVVLA
                     GWQVIQQRVAQYMAAFLIMSGLINGAFAALDAILFYVFFEAMLIPMYLIIGVWGGPRR
                     VYASIKFFLYTLLGSLLMLVAFIYLYQKAGQSFEILKFHTLAIPLSVQITLFVAFFLS
                     FAVKVPMWPVHTWLPDAHVEAPTGGSMVLAAITLKIGAYGFLRFALPILPDASRELAP
                     LMVGLSLVAVVYIGLVALVQTDMKKLVAYSSISHMGFVTLGMFLFTGNQLNQWAVEGA
                     LVQMVSHGFVSAAMFMCIGVMYDRLHTRNIADYGGVANKMPIFAAFMMLFAMANSGLP
                     ATSGFVGEFMVIMGAVQVNFWVAAAAATTLIFGAAYTLWMYKRVIFGDVGNSHVAEME
                     DVNKREFLVLAILAVAVLGMGLLPDLFVSKMHLSVNDLIAHVAHSKLQ"
     gene            18316..19773
                     /gene="nuoN"
                     /locus_tag="PKN55_01520"
     CDS             18316..19773
                     /gene="nuoN"
                     /locus_tag="PKN55_01520"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008953439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HNX69953.1"
                     /translation="MSWADLNLMPALPEIFLLSAVSVILVLDLFLSDKQRGITYLLSL
                     LSLIGCAVITLTTSQPFPELTFSGMFVDDPMADLAKVGMYVAMGATLVYSRQYIADRG
                     FYRGEFFTLSLFMLLGMMVMVSAHHFLTLYIGLELMSLALYALVALRRDSTQATEAAM
                     KYFVLGALASGMLLYGMSMVYGATGSLDIGKIAAALTNGSANMSLAMFGLVFIVAGLA
                     FKLGAVPFHMWVPDVYQGAPTAVTLLIGGAPKLAAFVFVVRILSQGLEPLVSQWQGML
                     VILAVLSMAIGNITAIAQTNLKRMLAYSTISHMGFLVLGILAGTPAGLSASLFYAVVY
                     VLMSLAGFGVLLLLSRAGVDCETLDDLKGLNRKNSWYAFLMLVVMFSMAGIPPMVGFN
                     AKLAVLKSVVDIGLIWLAVVGVVFSLIGAFYYLRVVKVMYFDEATDAEPVSQVGMDMR
                     VLLSVNAIALLVLGIVPDGLYSVCVEAMKQSLQVL"
     gene            19920..20213
                     /locus_tag="PKN55_01525"
     CDS             19920..20213
                     /locus_tag="PKN55_01525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017509617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2818 family protein"
                     /protein_id="HNX69954.1"
                     /translation="METRMAIVLLCAALAANLPFILNKVAGVWAVPRKHVGWCLLELG
                     VLYFAVGGLAYLLESQVSPVHVQKWQFYVTTFSLFLVLAFPGFIARFFWKRRA"
     gene            complement(20307..>20428)
                     /locus_tag="PKN55_01530"
     CDS             complement(20307..>20428)
                     /locus_tag="PKN55_01530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003684220.1"
                     /note="Acylates the intermediate (KDO)2-lipid IVA to form
                     (KDO)2-(lauroyl)-lipid IVA; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lipid A biosynthesis lauroyl acyltransferase"
                     /protein_id="HNX69955.1"
                     /translation="ADTRRMNAFIEERVREDPSQYYWLHKRFKTRPPGEARFY"
BASE COUNT         3676 a         6002 c         6388 g         4362 t
ORIGIN      
        1 gtcgccagac aaaacgccca gggtgtgagc cctgggcgtt tgtgctttgc ggggagcgga
       61 tcgcgcggga tcagccgaat ttgccggtga tgtagtcttc ggtggccttt tgcttgggcg
      121 cggtgaagat tgcgtcggtt tcgccaaatt cgatcaggtc gcccaggtac atataggcgg
      181 tgtagtcaga cacccgcgcc gcctgctgca tattgtgggt gacgatggcg atggtgtagt
      241 tctctttcag ctcatgcacc agttcttcga tctgggcggt ggaaatcggg tccagcgccg
      301 aggtcggttc gtccagcagc agcacttccg ggcggttggc cacggcgcgg gcgatgcaca
      361 gacgctgttg ctggccgccg gacagcgagt ggccggattg cttgagcttg tttttgactt
      421 cttcccacag cgcggctttt ttcagcgccc attcgatgcg gtcgtccatt tcgctcttgg
      481 gcagacgctc gtacagcttg atgccaaagg cgatgttgtc gtagatcgac atcgggaacg
      541 gggtgggctt ctggaacacc atgcccacct tggcgcgcag caggttgacg tcgatgtcgc
      601 ggccgaggat atttttgccg tccagcagca gctcgccttc cgcgcgcagg ttcgggtaca
      661 gctcgaacat gcggttgaag atgcgcagca gggtggattt gccgcaaccg gacgggccga
      721 tgaacgcagt caccttgccg gccgggatgt tcatgttgat gttcttcagg gcgtggaaag
      781 cgccgtagta aaagttcaga ttcttgacct ggagcttgat ggagtctgtc atggtgttca
      841 cgccttagtg cgaagaagtt tgctggctgc cgaaccagcg ggcgaggatg ttgagggtca
      901 gcacgctgaa ggtgatcagc agcgagcctg cccaggccag ggtgtgccag tcttcatacg
      961 gactcatggc gaactggaag atcacaatgg gcaggttggc catcggcgcg ttcatattgg
     1021 agttccagaa ctggttgttc agcgcggtga acagcagcgg ggcggtttcg ccggaaatcc
     1081 gcgccaccgc cagcaggatg ccggtgatca cgccagcctt ggaggcgcgc agggtgaccg
     1141 acatgatcac cttccattgc ggcgcgccca gggcggcggc ggcttcgcgc aggctgttgg
     1201 gcaccaggcg cagcatgttt tccgtggtgc gcaccaccac cggaatcacc agcagcgaca
     1261 gggccagcga gcccgcccag ccagagaaat gccccaccga caccacgtac acttcgtaaa
     1321 tgaacaggcc aatcacgatg gacggggccg acagcaggat gtcgttgata aagcgcgtgg
     1381 tcggcgccag ccagccacgg ttgccaaatt ccgccaggta gacgccggcc agaatgccca
     1441 ccggggtgcc gatcagcgtg ccaaacagcg tcatcagcag gctgccgtaa atggcgttgg
     1501 ccaggccgcc ctgactgccc ggcggcgggg tgatctgggt gaacacgtcc aggctcaggc
     1561 cgcccaggcc aagtttgagc agggtgtaaa gaatccacac caaccagaac aggccaaagg
     1621 ccacggtcag gaaggacatg gttttgttga agctgtttac cagccggcga cggcggtaca
     1681 gtgcgttatc catggttcag gtcctttgtt cttctttggc tcaggattcc ttgccttcgc
     1741 cagccttcag acgcagcagc agcagcttgg agcaggccag cacgacaaag gtgatgaaga
     1801 acaggatcag gcccaattca atcagggccg acaggtagag ttcgccttca gcttcggcaa
     1861 attcattggc cagcgacgag gcaatcgagt tggacgggtt gaacagacca gcggagattt
     1921 ggtaggcgtt gccgatgacg aaagtcaccg ccatggtttc gcccagcgcg cggcccagac
     1981 cgagcatgat gccgccgact acaccggttt tggtgtaggg caacaccacg tggcgcacca
     2041 cttcccaggt ggtggcgccc aggccgtagg cggattcttt cagcatcggc ggcaccactt
     2101 caaacacgtc acgcatcacc gaagcgatga acggaatcac catgatgccc agaatcaggc
     2161 cggcggtgaa cacgccaatg cccatcggca ggccctgaaa cagcaggcca atgccaggga
     2221 tggcgcccag ggtttccatc atccacggct gcacatggtc ggcaaaccac ggggcaaaca
     2281 cgaacaagcc ccacatgccg taaatgatcg atgggatgcc ggccagcagc tcaatggcca
     2341 cgcccagcgg gcggcgcagt tgcagcgggc atagttcggt gaggaacatg gcgatgccaa
     2401 agctgaccgg cacgccgatg atcagggcga tggtggcggt aatcagcgtg ccgaaaatcg
     2461 gcaccagtgc gccaaactcc tgcgccaccg ggtcccagga ggcattggtg agaaagccaa
     2521 ggccaaagtg cttgatggcc ggcgtggcgc cctggatcag cgagagcagg atggcggcca
     2581 gcagggccag caccagaaag gcaaagcagc gcgtgatcag ccggaacagc ggatccaggt
     2641 tggtgcgcgg cttttgcgtc gagctagaca gttgagtgct cataaaagcg tcttatcctg
     2701 atgatgcgaa agccggggtg gcgccccggc ttctgaatgt cgacaggtca agcccggtat
     2761 caggccggac ttgcgccagc cgggattact tccagaccgg cttgccgctg gtgtcggtga
     2821 tttgcttcca gctgtcgcga atcaccttct tgacgttgtc cggcatcggc acgtagtcca
     2881 tgtcggaggc cagcttgtca ccatggctgt aggcccagtc gaagaacttc aggctttcca
     2941 gcgattgttc cggcttgtcg gcctttttgt gcatcaggat gaaggtggcg cccgtgatcg
     3001 gccagctgtc cttgcccggt tgattggtca ggatttgata aaagccatca gcggctttcc
     3061 agctggcgtt ggcggcggcg gccttgaagg tcaggtcatc cggctgcaca aagctgccag
     3121 cctggttttt caccgcagcg tgggcaatct tgttttgctt ggcgtaggcg tattccacgt
     3181 agccgatggc gcccttgatg cgggtcacgt agttggccac gccttcgttg cccttgccac
     3241 ccaccccggt cggccagttg acggcggagt tttcacccac tttggtcttc cattcttcgg
     3301 agacgcgcga caggtagttg gtccagatga aggtggtgcc cgagccgtcc gagcggcgca
     3361 ccacggtgat gttctgtgcc ggcaggttca cgcccgggtt gatgctggtg atggccgggt
     3421 cgttccattt ggtgaccttg cccaggtaga tgtcagccag cagcgggccg gtgactttca
     3481 gctggcccgg cgccacgccc ggcaggttga ccaccggcac cacgccaccc atcaccgccg
     3541 ggaactgagt caggcccgcc ttgttcagct catccagggt cagcggtttg tcagaagcgc
     3601 cgaaatccac cgtcttggcc tgaatttgct tgatgccgcc acccgaaccg atcgactggt
     3661 agttcatgcc cacgccgctc ttggccttgt aggtttccgc ccatttggcg tacagcggat
     3721 acgggaaggt cgcgcctgcg ccggtgatgt cggcggcagc ggcgtagccg gagaacatgg
     3781 cgctgctcag cgtcatggcg gcaaccaggc gaatggtgga tttcatgcgg tgctcccagg
     3841 ggatgaatga gttattgcaa agcggtagcc atcgtagcgc ttcaaaattg cagatatatt
     3901 acaacacggt ttcatctttg ccccgagttg gcgacgatgg cttgaccgga tgcggcgcat
     3961 acctataatg ccgctcggtg tctgctgttg cgcaatgggg tgtggcggcg ggaaattttc
     4021 acgtttagag agtgcttcat gctgaatgtc gcgaccggaa ccaaatgggt gctgggcaac
     4081 tggaaaatga atggccggct ggccagcaat caggccctgc tggcggcctt gctgactgac
     4141 gcccaggtga acgtcgccca tgttggcctg gccgtgccga ccgtgtatat ggcgcaagtg
     4201 gcgcaggcct tgtctggtca gccgatggcg tggggcgggc aggcggtcag ccgctttgcc
     4261 gccgatggcg cctacaccgg cgaggtcagc gccgccatgc tggctgatct gggtgcgagc
     4321 tttaccctgg tcgggcattc cgaacgccgc cagtattttg ctgaaagcga cgaggttttg
     4381 tcggcgcaaa tctgccatgc gcgcgaggcc ggcttgctgc cggtggtgtg cgtgggcgag
     4441 ggcctggccg cgcgcgaagc gggcgagcac gaggcggtgg tggctgcgca gattgacggc
     4501 gtgattgctg cattgcaagg cagtgagcgg gtggtggtgg cgtatgagcc ggtctgggcg
     4561 attggcaccg ggctgaccgc ttccttggga caaattgtcg caatgcacga aaagattcgg
     4621 gcctgcctct tggaaaaatt ggcttcctcg tgtactattc tggttcttta cggcggcagc
     4681 gtgaaggcag ataacgccgc agacatcctc gctttgagcg aagtggatgg cgtgctggtc
     4741 ggtggggctt cgctggttgc ggagcaattc agcaaggttt gctacggcgc tgccaaatag
     4801 ataaaatatg gaaattctca aaacgctggt ctggattgtg aatatggctg ccgccctggc
     4861 agtgatcgtg ctcgtcttga tgcagcacgg caagggcgcg gacatgggcg ctgcttttgg
     4921 cagcggctcc tctggcagcc tgtttggcgc atccggctcg gccaatttcc tcagccgcac
     4981 caccgcagtg tgtgccgcta ttttcttttc gacttgcctg gcgttggtgt ttctctccac
     5041 gccgaccccc agcaacacgc tgggcgtgat gtccgggcaa gtggaaaccg ttgcgccggc
     5101 ctctcctgcc gcgcagccga atggtcaaaa caagccgggc cagatcccgg actgataaga
     5161 ataaagccga catggtgaaa ttggtagaca cgctatcttg agggggtagt ggcgaaagct
     5221 gtgtgagttc gagtctcact gtcggcacca tattcgaata ggtcgtgttc tgcggcctat
     5281 ttttaaaaca aaggaggctt ctggcctcct ttttttaggg gtaatcggga aatgctggag
     5341 aattactttc ccatcatgct gttcgtcatc atcgggctcc tggttggtgt aggacccatg
     5401 ttgattggtt tcttgttggg gcctcgtcgt cccgatcctg agaaactttc tccctacgag
     5461 tgtggttttg aggcgttcga agacgcccgg atgaagtttg acgtccgtta ctacctcgtt
     5521 gccatcctct tcatcctgtt cgacctcgaa attgcctttt tgttcccgtg ggcagtcgtg
     5581 ctcaaggaaa tcggcatgtt cggcttcgtc gccatgctgg tcttcctcgc gattctcgtc
     5641 gtcggcttca tctacgagtg gaaaaaagga gcgctggaat gggagtagaa ggcattctgg
     5701 aaaaaggctt tgtcaccacc acggctgaca aggtcatcaa ctggacccgc accggctcgc
     5761 tgtggccgat gaccttcggt ctggcgtgct gtgcggtgga aatgatgcag gccggcgcct
     5821 cgcgctatga cctggaccgc tttggcgtcg tgttccgccc cagtccgcgt cagtctgacc
     5881 tgatgatcgt cgccggcacg ctgtgcaaca agatggcgcc ggccctgcgc aaggtgtacg
     5941 accagatggc cgaaccgcgc tgggtgctgt cgatgggctc ttgcgccaat ggcggcggct
     6001 actatcacta ctcttactcc gtcgtgcgcg gctgtgaccg ggtggtgcct gttgatgtct
     6061 atgttccggg ctgtccgccc acggctgaag cgctgctcta cggcatcatc cagctgcaga
     6121 acaagatcaa acgtacttac actatcgccc gctaaaggtt aagcccatgg cctccacgct
     6181 ggaagcgctc aagtcggcgg cagaagccgc gctgggcgcc aacatcctct cagcgaccat
     6241 cgaccgtcac gagctgacgc tggtgtgtcg cgctgacacc atcatcgaaa cctgtaccct
     6301 gctgcgcgac acgccgtcgc tgcgtttcga gcagtgcgtc gacctgtgcg gcatggatta
     6361 cagcgcctat cgcgacggcg tgtgggaagg ctcccgcttt gccgtggttt gccatctgct
     6421 gtcggtggcc aacaaccagc gcgtgcgcgt gcgtttcttt gccactgatg acgattttcc
     6481 ggtggttccc tcgctggtcg atgtctggcc gggcgtcaac tggttcgagc gcgaagcgtt
     6541 cgacctgtac ggcatcattt tcgacggtca ccccgacctg cgtcgcctgc tgaccgacta
     6601 tggctttgtg ggccatccgt tccgcaagga cttcccgctg tccggccacg tggaaatgcg
     6661 ctacgacccg gttcaggcgc gcgtgatcta ccagccggta accatcgagc cgcgcgaaaa
     6721 tacgccgcgc atcattcgcg aggagaacta cggtggctga aatccgtaac tacacgctga
     6781 actttggtcc gcaacacccg gcggcgcacg gtgtgctgcg cctgattctg gagctggacg
     6841 gcgaagtggt tcagcgcgcc gacccgcacg tcggcctgct gcatcgcggc accgaaaaac
     6901 tggccgaata caagaccttc ctgcaatccg tgccgtatat ggaccgtctc gactatgtgt
     6961 cgatgatgtg caacgagcac gcctacgtga tggccatcga aaagctggtc ggcgtggaag
     7021 tgccgattcg cgcccagtac atccgggtga tgttcgatga aatcacccgc attctgaatc
     7081 acctgctgtg gatcggcgcg cacgcgctgg acatcggcgc catgaccatg ttcctgtacg
     7141 ccttccgcga gcgggaagat ctgatggacg tctacgaagc ggtgtcgggc gcgcgcatgc
     7201 atgcggccta ctaccgtccg ggcggcgtgt atcgcgatct gccggacgcc atgccgcagt
     7261 acaaggtctc caagatcaag aacgccagcc agctggccaa gctcaacgaa aaccgtcagg
     7321 gttcggtgct ggacttcatt gaagacttcg ccaaccgctt cccgacctat gttgacgaat
     7381 acgaaaccct gctgaccgac aaccgcatct ggaagcagcg caccgtcggc atcggcgtgg
     7441 tcacccccga gcgcgcgctg gcgctgggct tcaccggccc gatgctgcgc ggctccggca
     7501 ttgcctggga cttgcgcaag aagcagccgt acgaagtcta cgaccgcgtc gatttcgaca
     7561 tcccggtggg cgtcaatggc gactgctacg accgctacct ggtgcgcgtc gaagaaatgc
     7621 gtcaggccaa ccgcatcatc aagcaatgcg tggaatggct gaaaaagaat cctggcccgg
     7681 tcatcactga caatcacaag gtggctccgc cgtcgcgggt gaacatgaag agcaatatgg
     7741 aggagctgat ccaccatttc aagctcttct ccgaaggcat gcacgtgccg gaaggcgaag
     7801 cttacgctgc ggtcgagcat ccgaagggcg aattcggcat ctatctggtg tccgacggcg
     7861 ccaacaagcc ttaccgcctg aagattcgcg cgccgggcta cgcccacctg tccggtctgg
     7921 atgaaatgac ccgcggccac atgattgctg acgtggtgtc catcatcggg acgcaagata
     7981 tcgtgtttgg ggagattgac cgctgatgtt gtcgccagat gcactgaaac agattgaccg
     8041 cgaagtcgcc aaatatccgg cggatcaggc gcggtcggcg gtgatgggcg ccctgcgcat
     8101 tgcccagacc gaactcgggt ggctgtccac cgagaccatc gaattcgtcg ccaactacct
     8161 gggcattccc ccggtggcgg cctatgaagt ggccactttc tacaatatgt acgacctcaa
     8221 gccggtcggc aaatacaaga tcaccgtctg caccaatttg ccctgtgcgc tgggcggcgg
     8281 ggtcgattgc gcggactacc tcaagcacaa gctgggcatt ggctttggcg aaaccacccc
     8341 ggatggcaag ttcaccctgg tggaaggcga gtgcatgggg gcatgtggcg atgcgccggt
     8401 gctgctggtg aacaaccaca gcatgtgcag cttcatgagc cacgaggcaa tcgacaagaa
     8461 actggcggag ttgaaataac atggccgtgt tcactcaagg cgtgattttc gaaggcgtcg
     8521 atacctctga cccgaattgc tggacgctgg acgcctacgt caagcgcgga ggctacgaag
     8581 cgctcaagcg catccttcag caaggcatct cgcaagatga cgtgattgct gaagtcaaag
     8641 gctccagcct gcgcggccgt ggcggcgcag gcttcccgac cggcctgaaa tggtcgttca
     8701 tgccgcgcaa ttttccgggc gacaaatatc tggtgtgcaa caccgatgaa ggcgaaccgg
     8761 gcaccttcaa ggaccgcgac atcctgcgct tcaatccgca ttcgctgatc gaaggcatga
     8821 tcatcgccgg ctacgccatg ggcgtgaagc gcgcctacaa ctacatccac ggtgaaatct
     8881 tcgaggatta cgtgctgttc gagcgtgcgc tcgaccaagc gcgcgccgcc ggcttcctcg
     8941 gcgaaaacat cctgggcacg agcttcacct tcgatctgtt tgcccaccac ggctacggcg
     9001 cctacatctg cggtgaagaa accgcgctgc tggagtcgct ggaaggcaaa aaaggtcagc
     9061 cgcgcttcaa gccgccgttc ccggcctcgt ttggtctgta cggcaagccg accaccatca
     9121 acaacaccga atcgttcgct tcggtgccgt tcatcattcg cgacggcggc cagaaattcc
     9181 tggaagccgg caagccgaac aatggcggca ccaagctgtt ctcggtgtct ggccacgtca
     9241 accgtccggg caactacgaa attcccttgg gcaccccgtt tgccaccctg ctggaaatgg
     9301 ccggcggcat gaaaaacggc aaaaagctca aggcggtgat tccgggcggt tcgtcggcgc
     9361 cggtgctgcc gggcgacatc atgatggact gcaccatgga ctacgacagc atcagcaagg
     9421 ctggttcgat gctgggttcg ggcgccgtga tcgtgatgaa cgaagacatc tgcatggtca
     9481 aggcgctgga gcgtctggcc tacttctacc acgaagaatc ctgcgggcag tgcaccccat
     9541 gccgtgaagg cacgggctgg ctgtatcggg tgattcaccg catcgagcat ggccagggcc
     9601 gtcccgacga cctcgacctg ctgacttcgg tgggcaacaa catggcaggc cgcaccattt
     9661 gcgcgctggc cgatgccgct gtcttccccg tccgcagctt caccaaacat ttccgccatg
     9721 agttcgagta tcacatcgaa cacaagaaat gtctggtcga gcacaaatgg tgctgatccc
     9781 ctgggatcgc ttgctacagg tagcgcaacg ctatgttaga aatcgaaatc gacggtaaga
     9841 aattgacggt gcccaccggg agcaccgtga tggatgcagc ccattccaat ggcacctaca
     9901 tcccgcattt ctgttaccac aaaaaactgt ccatcgccgc caactgccgc atgtgcctgg
     9961 tggaagtgga aaaagcgccc aaaccgctgc ccgcctgcgc cacgccggtg accgacggca
    10021 tgaaggtgca cacccactcc gacaccgcgg tgaaggcgca gcagggcgtg atggaattcc
    10081 tgctgatcaa ccacccgctg gattgcccga tctgcgacca gggcggcgaa tgccagctgc
    10141 aagatctggc cgtggggtat ggcggcgtca gctcgcgcta ccaggaagaa aagcgcgccg
    10201 tgccggccaa ggaaatgggc ccgctggtgt ctgcggcgga aatgagccgc tgcattcact
    10261 gcacccgctg cgtgcgcttc accgaagaaa tcggcggcta tcaggaaatc ggcatggctc
    10321 accgtggtga gcactcgcag attctgccct tcctgaacaa aaccgtagac tcggaaatct
    10381 ccggcaacgt catcgacctg tgcccggtgg gcgcgctgac cagcaagccg ttccgctaca
    10441 gcgcccgtcc gtgggaactg tcgcgccgca agacggtgag cccgcacgat ggcctgggcg
    10501 ccaatatgat cgtgcaggtc aagcacaacc gcgtgatgcg cgcgctgccg ctggaaaacg
    10561 aagccatcaa cgaatgctgg ctggccgacc gcgaccgctt ctcctacgaa gcgctgtcca
    10621 gcgacgcccg tctgacccgt ccgatggtca agcaaggcgg ccagtggatc gaaaccgaat
    10681 ggcaagccgc gctggaatac atcgtcaagg gcctgcgcgg cgtcacccgc gaccacggcg
    10741 ctgacgcgat tggctttttg gccaccccgc acagcacggt ggaagaactc tacctggcca
    10801 agaaactggc cggcgcgctg ggcgtggaaa gcacggacta ccgcacccgc cacgccgact
    10861 tcagccgtga cgccgcccag cagggcgcgc tgtggctggg ctccagcatc gtcgagttgg
    10921 ccgccgccaa tgcgctgctg gtggtcggct cggtgctgcg caaagagcag ccgctgctgg
    10981 ccgcgcgcct gcgtcagtcg gtcaagaagg gcatggcgct gagcgtgatc agcccgattc
    11041 aggaaaacct gctcagccat gtgcacgccc gcctgaccgt cagcccggtg gccatgctgg
    11101 attcgctgaa agccgtgctc aaggccgtgc tggcccgcaa ggccgacgcc agctcgacgc
    11161 tggacgtggt gtcgatcacc ccgtgtgcgc aagccgaagc catcgccgac agtctggtga
    11221 acaatgacaa cgccgtgatt gccctgggca atctggcgca gcacagcccg cactttggcg
    11281 acctgctgac cctggccaac gaactggccc gcctcaccgg cggccgcgtg gccgtgctgg
    11341 gcgaagccgc caacagcgtc ggcgcggaac tcaccttcaa gcccagcggc aagggcgcca
    11401 acgccggcca gatgttctcg gtgccgaaga aggcctatgt gctgctgaac accgaactgg
    11461 actgcgacag ccacaacccg catcgcgcgc tgaaggccgt ggagcaagcc gagatggtgg
    11521 tggcgatgtc cgccttccgt tcggacgccg cgctgcaata cgccgatgtg atgctgccga
    11581 ttgccccgtt caccgaaacg tccggcgcct tcgtcaatat ggaaggcctg ctgcaagcgt
    11641 tcaacggcgt ggtcaagccg ctgggcgacg cccgtccggc ctggaaggtg ctgcgcgtgc
    11701 tgggcaatat gcttggcgtg ccgggcttcg agtttgagtc cgccgagcag gttcgcgcgg
    11761 aatggctggc tggcttgggc ggcgagctca agagcaagct gaacaatggc ctgagcagcc
    11821 tgacgctggc gacgccgtct tcggccagcg gtctggtgcg cgtgggcgaa gtgccgatgt
    11881 atcaggccga cgcgctgtgc cgtcgcgcgc cgtcgctgca agccaccgcc gatgcccgcg
    11941 ccccgcgcgc cagcgcacat tcgagcgtgt tggccgccgc tggcgtgcag tccggtcagg
    12001 aagtcagcct caagcaggaa ggcgccgcca tccgcatcgc cattgatgca gatgacagcc
    12061 tgccgcttgg cgtggttcgt ctggccgctg cccacccggc aaccgtggcg ctgggcggta
    12121 tgttcaacag cattgaactg gcgcaggggt aaagcatgga attctttcag aatctcctcg
    12181 gagcggatgc aggtctggca gtttggaccg tgctgaagat cgtggcaatt gtcgggccga
    12241 tgatgggggc tgtggcctac ctgacgctgg ccgaacgtaa ggtgatcggc tatatgcaga
    12301 tccggatcgg cccgaaccgg gtcggcccca agggtctgct gcaaccgatt gctgacggca
    12361 tcaagctgat gctcaaggaa atcgtgctgc cggccaaggc gaataaaacc ttgttcctgg
    12421 tcggcccgat cctgtcgatt gccccggcgc tggccgcctg ggcagtggtg ccgttcatgc
    12481 ccaagctggt gctggccaat atcgacgcct cgctgctgta catcatggcg gtgacctcga
    12541 ttggcgtgta cggcgtgatc gtcgccggct gggcgtccaa ctccaagtac gccttcctgg
    12601 gcgcgttgcg ttcggcagcc cagatcgtgt cctacgaaat cgccatgggc tttgccttgg
    12661 tgggcgtgtt gatgctgtcc ggttcgctga acctggtgga aatcgtcaac cagcaaggtc
    12721 agggcattgc gggcggttcg gtgttctcct ggaactggct gccgctgttc ccgatgttcc
    12781 tggtctatct gatctccggt gtggcggaaa ccaaccgcgc cccgtttgac gtggccgaag
    12841 gcgaatcgga aatcgtggcc ggcttccacg tggaatactc gggcatggcg ttcgcagtgt
    12901 tcttcctggc cgaatacgcc aacatgatcc tgatcgcctc gatgaccgcg ctgatgttcc
    12961 tgggcggctg gctgtccccg ctgccggctt cggtgccggt gctgggtgaa agcggcttcc
    13021 actggctggc gatcaaggct gcgttcatcc tcttctgctt cctgtggttc cgcgcgacct
    13081 tcccgcgtta ccgctatgac cagatcatgc gtctgggctg gaaagtgttc attcctgtga
    13141 ccctggtgtg gctggttgtc gtcggtttct ggatgatgtc cccgtacacg ctgtggaaat
    13201 aaccggtaag tgagtggcat ggaaacgatc cgcaatttct tcaagacatt ccttctcgtc
    13261 gagctcgtca agggcctggc gttgaccggt cgtcatttct tccagcgcaa gatcactgtg
    13321 cagtttccgg aagaaaaaac gccgcaaagc ccgcgttttc gcggtttgca tgcgcagcgc
    13381 cgctacccca acggggaaga gcgctgcatc gcctgcaaac tgtgcgaggc ggtctgcccg
    13441 gccatggcga tcaccattga gtcggaacag cgcgacgatg gcacccgccg cacttcgcgt
    13501 tatgaaatcg acctgaccaa atgcattttc tgcggcttct gtgaggagtc ctgcccggtg
    13561 gattcgattg tggaaaccca catcctcgaa taccacggcg aaaagcgcgg tgacctgtat
    13621 tacaccaagc aaatgctgct ggccgtcggt gaccaatatg aagccgagat cgcgaaaaat
    13681 cgcgagctcg acgccaagta ccgttaaggg ggccacatgt cgttccaagc atttgtgttc
    13741 tacgtgctct ccgccattct ggtgtttgcc ggggtgcggg tgatcacggc caaaaacccg
    13801 gtgcacgctg cgctgttcct ggtgctggcg ttctttaccg cctcaggcca ctggctgctg
    13861 ttgcagtcgg agttcctggc catctcgctg gtgctggtgt atgtcggcgc ggtgatggtg
    13921 ttgttcctgt tcgtggtgat gatgctcgac atcgacttcg agcagctgcg tgaaggcttc
    13981 tggcgcaatc tgccggtggc cggcacggtg gccgccatca tggtggcgga gatggtgctg
    14041 atcctgacca gtccggaatc caatctgaac ggcttcaaag agccggcggc gctggcggcg
    14101 gactacagca acgtcaagga cctgggtcgt cagatttaca ccacgtatct gtatccgttt
    14161 gaactcgccg ccgtgctgct gctgctggcg attgtcgcgg cgattgcgct gaccatgcgc
    14221 aagcgcaagg acaccaagtg ggtggatccg gccgaacagg tcaaagtgcg tcgcaatgat
    14281 cgcgtgcgca tgatccagat ggctgccgaa ccgcgtgtgg aagatcgcgg cggcgacacc
    14341 aataacgaca acgcagcggc ctgaccgcag caagccctaa tccaagcaaa gggggtaccg
    14401 tgttgactct gacgcattac ctggtgctcg ccgcgattct gttcgcgatc agcgtgctgg
    14461 gtatttttct gaaccgcaag aacgtgattg tgctgttgat ggcgatcgag ctgatgctgc
    14521 tcgccgtgaa ctacaacttc atcgccttct cgcactatct gtccgacact gcgggtcaga
    14581 tcttcgtgtt cttcatcctg accgttgccg ccgctgagtc ggcgattggt ctggccatcc
    14641 tggtggtgtt gttccgcaat ctgcgcacga tcaacgttga agacctaggc agcctcaaag
    14701 gctaaagacc aaccggaatc ctaagaaaag aagctcgata agcatggaaa tgaaaagctt
    14761 gtacctgctg attgcgctgg cgccccttgc gggctcagcc attgcggggt tattgggttg
    14821 ggcgatcggc cgcgttgccg cgcacctcgt caccattggt ggcgtggcgg tgtcggcggt
    14881 gggcgctgtc tatgtgctca acggtctgct caacggggcg cccactttcg atggcccggt
    14941 atacacctgg ctgacggtcg gcggcattga ttattcggtc ggtttcctga tcgacaacct
    15001 caccgccatg atgatggtgg tggtcaccag cgtgtcgctg atggtgcaca tctacaccat
    15061 cggctatatg catgaagatc cgggctatca gcggttcttc agctacatct cgctgttcac
    15121 cttctcgatg ctgatgctgg tgatgagcaa caacttcatc cagctgttct tcggctggga
    15181 agcggtgggt ctggtgtcct atctgctgat tggtttctgg ttcaagaagc cgacggcgat
    15241 tttcgccaac ctcaaggctt tcctggtcaa ccgcgtgggt gactttggct tcctgctggg
    15301 catcggcatg gtgctggcct acttcggcgg ctcgctgcac tacgccgacg tgtttgccgc
    15361 tgcgcctggc ctggctacga agactgtgca gattttccct ggcgtcgaat ggtcgctgat
    15421 gacggtgacc tgcatcctgc tgtttatcgg cgcgatgggt aaatccgcac agtttccgtt
    15481 gcacgtctgg ctgccggact cgatggaagg cccgaccccg atttccgcgc tgatccacgc
    15541 cgccaccatg gtgacggccg gcatcttcat ggtgtcgcgc atgagcccgc tgttcgagct
    15601 gtccgacacc gcgctgtcct tcatcctggt gattggttcg atcaccgcgc tgttcatggg
    15661 ctttctgggc atcatccaga acgacatcaa gcgtgtggtg gcttactcca cgctgtcgca
    15721 gctgggctac atgaccgtcg ccctgggcgt gtccgcctac ccggtggccg tgttccacct
    15781 gatgacccac gccttcttca aggccctgct gttccttggc gccggttcgg tgatcatggg
    15841 catgcaccat gaacaagaca tgcgccgcat gggcggtctg tggaaataca tgccggtgac
    15901 ctggttcacc tcgctgctgg gctcgctggc gctgattggc accccgttct ttgccggctt
    15961 ctactccaag gattcgatca tcgaagccgt ggccgcctcg catattccgg gcagcagctt
    16021 tgcgtacttt gctgtggtgg ctggcgtgtt tatcactgcc ttctattcct tccgcatgta
    16081 cttcctggtg ttccacggtg aagagcgttt ccgccacgtg catcatgacg accatcatgg
    16141 tcatcacgac gatcacggcc acggtcatgc ccatgtgccg cacgaatcgc cgtgggtggt
    16201 gaccctgccg ctggtgctgc tggccatccc gtcggtgatc attggcttca tcgccatcga
    16261 accgatgctg ttcggcgact tcttcaagaa cgtgatcttc atcaatcacg aagcccatcc
    16321 ggtgatggaa gagctggcgc acgagttcca cggcgccgca ggcatggcca cccatgcgct
    16381 gagcaccctg ccgttctggc tggcgctggg cggtgtggcc acggcatggc tgttctacat
    16441 gaaggccccg cagattccgg cgatgttcaa ggccaagctg ggcgccatcc acaccctgct
    16501 ggaaaacaaa tactacctcg acgacatcta ctttggcgtg ttcgccaagg gctcgcgcgg
    16561 cctgggcacc ctgctgtgga aggtgggcga ccagctgctg atcgatggcc tgttggtcaa
    16621 cggttcggcc aaggtggtgg gggcgttctc ggctgtggtg cgcaagctgc aaaccggctt
    16681 catctacagc tacgcgaccg cgatgattat tggcgttctt ggtctcatga cgatctggtt
    16741 ttctggcctg atcgcccact gagatgccga cggatacatt cagaaaaaca gttaggttgg
    16801 gcttatggtt gagaatctgt taagtctggc aatctggacg cctatcgtgg ccgggttgct
    16861 ggtgctggca acaggagggg acaaaaatgc cggtctggcg cgctgggtag cgctgctggg
    16921 ctcgctggcc ggctttgtcg tctccatccc cctgtttacc ggcttccagt cgctgaacgg
    16981 aggcatgcaa ttcgtggaga aagcgccatg gattgccagc ctgaacattc actactacct
    17041 gggcgtggac ggcatctcga tgctgttcgt ggtgctcaac agcttcacca ccgtcatggt
    17101 ggtgctggct ggctggcaag tgatccagca acgtgtggcg cagtacatgg ctgccttcct
    17161 gatcatgtcg ggcctgatca acggcgcgtt tgccgcgctg gatgcgatcc tgttctacgt
    17221 gttctttgaa gccatgctga tcccgatgta cctgatcatc ggtgtctggg gcggcccgcg
    17281 ccgcgtgtac gcgtcgatca agttcttcct gtacaccctg ctgggttccc tgctgatgct
    17341 ggtggctttt atctacctgt atcagaaagc gggtcagagc tttgaaatcc tgaagttcca
    17401 caccctggcg attccgctgt cggtgcagat caccctgttc gtcgccttct tcctgtcgtt
    17461 cgcggtgaag gtgccgatgt ggccggtgca cacctggctg ccggatgccc acgtggaagc
    17521 gccgactggc ggctccatgg tgctggccgc catcaccctg aagatcggcg cttacggttt
    17581 cctgcggttt gccctgccga ttctgccgga cgcctcgcgt gagctggctc cgctgatggt
    17641 gggcctgtcg ctggtcgccg tggtgtacat cggcctggtg gcgctggtgc agaccgacat
    17701 gaagaaactg gtggcgtact cgtcgatctc gcacatgggt tttgtcaccc tgggcatgtt
    17761 cctgttcacc ggcaaccagc tcaatcagtg ggccgtggaa ggcgcgctgg tgcagatggt
    17821 gtcgcacggt tttgtgtccg ccgccatgtt tatgtgcatc ggtgtgatgt atgaccgtct
    17881 gcacacccgc aacatcgccg actacggcgg tgtggccaac aagatgccga tttttgccgc
    17941 cttcatgatg ctgttcgcca tggccaactc cggcctgccg gccaccagcg gttttgtcgg
    18001 cgagttcatg gtgatcatgg gcgccgtgca ggtcaacttc tgggtggctg ccgccgccgc
    18061 caccacgctg atctttggcg cggcttacac cctgtggatg tacaagcggg tgatttttgg
    18121 cgacgttggc aattcgcacg tcgccgagat ggaggacgtc aacaagcgtg aattcctcgt
    18181 gctggccatt ctggctgtcg cggtgctggg catgggtctg ctgcctgacc tgttcgtgtc
    18241 caagatgcac ctctcggtca acgatctgat cgcccatgtg gcgcacagca aactgcaata
    18301 agaggataaa tcacgatgag ttgggctgat ttgaacctga tgccggcctt gcccgagatc
    18361 ttcttgctct cggcggtgtc ggtcatcctg gtgctggacc tctttctttc cgataaacaa
    18421 cgcggcatca cctatctgct gtccttgctg tcgctgattg gctgtgccgt catcaccctg
    18481 accaccagcc agccgtttcc ggagctgacc ttcagcggca tgttcgtgga tgatccgatg
    18541 gctgacctgg ccaaggtggg catgtatgtc gccatgggcg ccaccctggt gtacagccgt
    18601 cagtacatcg ccgaccgcgg cttctaccgt ggcgagttct tcaccctgtc gctgttcatg
    18661 ctgttgggca tgatggtgat ggtgtctgct caccacttcc tgaccctgta tatcggtctg
    18721 gagctgatgt cgctggcgct gtacgcgctg gtggccttgc gccgcgactc gacgcaggcg
    18781 actgaagcgg cgatgaagta cttcgtgctg ggcgcgctgg cctcgggcat gctgctgtac
    18841 ggcatgtcga tggtgtacgg cgccaccggt tcgctggaca tcggcaaaat cgccgcggcg
    18901 ctgaccaacg gctcggccaa tatgtcgctg gccatgtttg gcctggtgtt catcgtcgct
    18961 ggtctggcct tcaagctggg cgccgtgccg ttccacatgt gggtgcctga cgtgtatcag
    19021 ggcgcgccga ccgccgtcac cctgctgatc ggcggcgcgc cgaagctggc ggcctttgtg
    19081 ttcgtcgtgc gcatcctgtc gcaaggtctg gagccgctgg tgtcgcaatg gcaaggcatg
    19141 ctggtgattc tggccgtgct gtcgatggcc atcggcaaca tcaccgcgat tgcccaaacc
    19201 aacctcaagc gcatgctggc gtactcgacc atctcgcaca tgggctttct ggtgctgggc
    19261 attctggccg gcaccccggc tggcctgtcc gcctcgctgt tctacgccgt ggtgtatgtg
    19321 ctgatgtcgc tggccggctt tggcgtgctg ctgctgctgt cgcgcgccgg tgtggattgc
    19381 gaaaccctgg acgacctgaa gggcctgaac cgcaaaaaca gctggtacgc cttcctgatg
    19441 ctggtggtga tgttctccat ggcaggcatt ccgccgatgg tgggctttaa cgccaagctg
    19501 gcagtgctca agtcggtggt ggacatcggc ctgatctggc tggccgtggt gggtgtggtc
    19561 ttctcgctga ttggcgcttt ctactacctg cgcgtggtca aggtcatgta ctttgacgaa
    19621 gcgactgacg ctgagccggt gagccaggta ggcatggaca tgcgcgtgct gctgtcggtg
    19681 aacgccatcg ccctgctggt gctgggcatc gtgccggatg gtttgtactc cgtctgcgtg
    19741 gaagccatga agcaatcgct gcaagtgctg taaagtcggc ttctggctgg cacttttgcc
    19801 cgccagcgcc gtcacaggtc atgccgtccg cgcgcgagcc cggacggcat gactgttttt
    19861 atattctttg atggtggatt tgccggcctg cgcgccggta gaccagaagc gggagactca
    19921 tggaaacgcg tatggcgatt gtgctgctgt gtgcggcgct ggcggcaaat ctgccgttca
    19981 tcctcaacaa ggtggcgggc gtctgggccg tgccgcgcaa gcatgtcggc tggtgtttgc
    20041 tggagctggg cgtgttgtac tttgccgtgg gcgggctggc ctatctgctg gaaagccagg
    20101 tgtcgccggt gcatgtgcaa aagtggcagt tttatgtcac cacgttttcg ctgtttctgg
    20161 tgctggcgtt tccggggttt attgcccgtt ttttctggaa acgccgcgct tgagcgcttg
    20221 ctgtgcgcgg taagccccaa caaaaaaccg ccaggcgatg tgactggcgg ttttttgttg
    20281 gatttcgggt gcggcgggct ggcggctcag taaaagcgcg cctcacctgg cggtcgggtc
    20341 ttgaagcgct tgtgcagcca gtaatactgg gaagggtctt cccgcacacg ctcctcgatg
    20401 aatgcattca tgcgtcgggt gtcggcca
//
LOCUS       DAOPYX010000014        20353 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_2721_length_20353_cov_8.196916, whole genome shotgun sequence.
ACCESSION   DAOPYX010000014 DAOPYX010000000
VERSION     DAOPYX010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 20353)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20353)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20353
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2721_length_20353_cov_8.196916"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..326)
                     /locus_tag="PKN55_01535"
     CDS             complement(<1..326)
                     /locus_tag="PKN55_01535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012697778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HNX69956.1"
                     /translation="MSEAPVVGKPKKSVALSGVAAGNTALCTVGRSGNDLHYRGYDIL
                     DVATTCEFEEIAYLLVHGKLPNQAELTGYKKKLKALRGLPAAVKTALEALPAASHPMD
                     VMRTAVS"
     gene            complement(411..1289)
                     /gene="prpB"
                     /locus_tag="PKN55_01540"
     CDS             complement(411..1289)
                     /gene="prpB"
                     /locus_tag="PKN55_01540"
                     /EC_number="4.1.3.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011135605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046421 - methylisocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylisocitrate lyase"
                     /protein_id="HNX69957.1"
                     /translation="MMTPGQRFKAAVAAEKPLQVVGAINAYAARLADHSGYKALYLSG
                     GGVAAGSCGIPDLGITTLEDVLIDVRRITDVTELPLLVDIDTGWGGAFNIARAIRHLE
                     KAGAAAVHIEDQVQQKRCGHRPNKAIVPLDEMVDRVKAAVDARRDNDFVIMARTDALA
                     VDGLQAAIDRACACVEAGADMIFPEAMTELAMYKQFVEAVKVPVLANITEFGSTPLFT
                     TEELASVGVSLALYPLSAFRAMNLAALNVYQTLRQEGTQKNVVPTMQTRMDLYDHLGY
                     HEYEQKLDALFAKEAK"
     gene            1748..1930
                     /locus_tag="PKN55_01545"
     CDS             1748..1930
                     /locus_tag="PKN55_01545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX69958.1"
                     /translation="MSVLILWNLFHWGSAAFIIVVLFFAFIGPPNKQGTPYTVAVQPQ
                     AAPANAPAEHSKKFNF"
     gene            1962..2924
                     /locus_tag="PKN55_01550"
     CDS             1962..2924
                     /locus_tag="PKN55_01550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX69959.1"
                     /translation="MIFISKQLVGCLLAFSLACLGTTAQAAECNGLKVATGPAGKGYA
                     RLFADMQQVCGRTVAMCELPTSGGLDNLHAMSANEADIGFAQVDTWADMQMSNENIAG
                     LQAVLSLNFNYLHVVVAANGFAVAGESRFGGLLRGKPQYVLMERFSSLRGQRVAVVGS
                     TALLGRKLEQRLRYGLHFVDVETDDQAFQLVTSDKVAAALTVSGWPSGTVGALKPDDR
                     LSLAPFDAPSDAPYVVRPVSYRGLGVYNTNMLAAPNVLLSRPFKGAKASQVAALKACV
                     ASHMLELQEGSYSPGWKEIKSLDKTWGIPPFKGAVAPAKTDRKK"
     gene            complement(3053..3718)
                     /locus_tag="PKN55_01555"
     CDS             complement(3053..3718)
                     /locus_tag="PKN55_01555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012697361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HNX69960.1"
                     /translation="MQYRIKLWDTPTRLFHWSLVLLFGVMWWSGETGGDALRLHLLAG
                     YGIAGLLLFRLLWGVLGSDTARFARFVRGPGAMLAYLRGSLPEHRIPGHNPLGALMVL
                     ALLGLLGFQVGSGLFATDVDSFLWDGPLAKLINSDLSEAITGWHKISFKLLLAAVGLH
                     LSAIVFYRLIKRRNLVVAMLSGWQTYASQPPELRFASWKLAVLCALVAAGVVAGVVNG
                     LGG"
     gene            complement(3819..4847)
                     /gene="trpD"
                     /locus_tag="PKN55_01560"
     CDS             complement(3819..4847)
                     /gene="trpD"
                     /locus_tag="PKN55_01560"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014087082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HNX69961.1"
                     /translation="MISAQAALNRLIDGNELFYDEMLDLMRQIMRGELTPAQIAAILI
                     GLRVKVESVSEIAAAATVMREFATPVPVAKREHLVDTCGTGGDGAHTFNISTTSAFVA
                     AAAGARVAKHGGRSVSSSSGSADVLETLGVKLNLSAEAVGQCIDDIGLGFMFAPNHHS
                     AMKHVAPVRRELGVRTLFNILGPLTNPAGADNQVMGVFHPDLVGIQARVLRQLGSRHV
                     LVVHGEDGLDEITLTGRTRVAELKDGAVSEYLLDPREFGLNYCDISALKASSAEESAT
                     RVRAVLAGETGPCRDIVLLNAGAAIYVAGVAATLSEAIDRARQAIDSGAAQRTLAALA
                     ERTQALAA"
     gene            complement(5023..5592)
                     /locus_tag="PKN55_01565"
     CDS             complement(5023..5592)
                     /locus_tag="PKN55_01565"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011135723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HNX69962.1"
                     /translation="MLLMIDNYDSFTYNLVQYFGELGQDVQVFRNDEISLDDIARLAP
                     QYLVISPGPCTPLEAGVSVAAIERFAGQIPILGVCLGHQSIGQAFGGKIIHAKQLMHG
                     KTSPVQHLDLGVFHSLPNPVTCTRYHSLVIERESLPDCLEITAWTEDGEIMGVRHKTL
                     AVEGVQFHPESILTQHGHTMLRNFLTAHA"
     gene            complement(5797..7464)
                     /locus_tag="PKN55_01570"
     CDS             complement(5797..7464)
                     /locus_tag="PKN55_01570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX69963.1"
                     /translation="MPPSTTLKVISAPACQTFDTLLNNARKAQKHDCWGDPQIRDNLS
                     YMTSEANLLAAVELAGARGALERARDAYKSENTAGYRGPAEILFDVDDNLNLPERLAR
                     FVRDVPGNTVSPPDENAIISSLERALSAPAKETTFQTLSCPACATFDQVLSGRLAFNK
                     LGPARDVPGVSSDWGMPQFRDNIQFLLVDPSCYALVRQQEAGTHLGWAWDAYQTNGDD
                     GEPASPQQYQQACGPVEDLFNTQSGACLSARLAAFVRATDGFLPRKDNDVMVSRMSPP
                     TLLQQATLPADLVALDSICQPLWPLLSSMSRQTGNSDWETWFSKQLAALDNRSPVQMK
                     DLPADGLLALLRDALPGLIAGKVISSLINRGLNAAAGQTVGLIGYSVLGFSGKAVTFG
                     SNVLAPWKATYQFSNLVKKQGPLFSAREQARQTLKSDADPDLKKLETCLNACLDSIDW
                     TAAKAAFEVTPFGLLTTVYSGVKWLVLKVSPTQNSYYGQACTLLDLAELGRNGQSELT
                     ASQQLARQLAQVVILHLCNGNLTRFLKLMTTNKDTVAYGELAKLIDG"
     gene            7629..9248
                     /locus_tag="PKN55_01575"
     CDS             7629..9248
                     /locus_tag="PKN55_01575"
                     /inference="COORDINATES: protein motif:HMM:NF020650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNX69964.1"
                     /translation="MHVISFPRLICRLCLILLLAPCLLLARPAWALTPVTLQLKWTHT
                     FQFAGYYAAQIRGYYREAGLDVTLREAQPGDDPLRAVLSGRAEFGVGTSSLLLARHRG
                     APVVVLAVIFQHSPLVLLSKSGGPSAGIHDLLDKRVMLEPQSDELLAYLQQEGISSRR
                     ITWLAHSYRPTALLDSGVDAVSAYSINEPYYLDQAGLQYQTFTPRSAGIDFYGDNLFT
                     REALLNDSPEMVRAFRTASLRGWQYAMAHPEEIIQWLVNTYPGRHPADFHRHEARRMA
                     PLLRTDLVEVGYMNPGRWRHIADTYAELGLLPSQFSLKGFLYQTKPAIDWLWLGGALA
                     VTAMIGGVALYIHRINRQLTRALTTSQATLNALYASEARLRELNARDALTGLANRERL
                     FEQLDTLLGAPQAQLAVLFIDLDGFKAINDQFGHAAGDQLLRVMAERMRASLPARALL
                     ARLGGDEFVAVLPEAAHATEHAQQLLAALAAPLPTCDCTLSASIGMACAPTDGHDAQT
                     LVQHADLAMYAAKRNGRNQLQRWPVAALARD"
     gene            9325..9963
                     /gene="pdxH"
                     /locus_tag="PKN55_01580"
     CDS             9325..9963
                     /gene="pdxH"
                     /locus_tag="PKN55_01580"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012697487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine-phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HNX69965.1"
                     /translation="MKNIADIRKDYASQTLDPDDCLAHPVAQFSRWLDDAIAADVPEP
                     TAMLVSTVSPEGRPAARVVLLKGVEDNQLVFYTNYHSRKGQHLGHTPFIALTFFWPEL
                     ERQVNIEGVVNRVADETSDAYFDSRPYKSRVGAWASEQSQPISGKEVVMARFARFAAQ
                     YLTHVPRPPHWGGFAVTPERMEFWQGRPSRLHDRVLYLPDGQGGWQKQRLAP"
     assembly_gap    9972..10071
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<10072..10939)
                     /locus_tag="PKN55_01585"
     CDS             complement(<10072..10939)
                     /locus_tag="PKN55_01585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX69966.1"
                     /translation="MTNGEHSPKEWSEKGLLAEIRKLHQQGRSLAFILGAGASASSGI
                     LTGGKLAYDWLQEWHQRECLEPDTDLQSWAREQLENPTFSTHQDAKPAYQDAASHYST
                     IFERRFAGDYTSGYAALEDAMEDAAPSVGYSILAKILDETHHRVVVSTNFDNLVADAL
                     AIHARRQPLIVGHEALAGFARPQLRRPLVAKIHRDLHLAPKNDREGVNTLGQGWGNSL
                     SQLFQYSTPLVLGYGGNDGSLMGFLNDLPAGHIPGRLFWCYRDEPPAQNVLATIAKHS
                     GVIVKISGFDEFM"
     gene            complement(11023..11754)
                     /locus_tag="PKN55_01590"
     CDS             complement(11023..11754)
                     /locus_tag="PKN55_01590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011135870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNX69967.1"
                     /translation="MTPTPNFDPIVYVVDDDPAVLDSVSLLIRSAGLKVMAFPSALEF
                     LAAFIPNQIACLVLDIRMPGTTGLELQAELEGAGVALPIIFITGHGDVAQCTRAFKAG
                     ASDFLTKPIDGELLMEAVRKALRHCIQTHEKTARTQEAEMRLARLSSRELEVLQLVTE
                     GLSSKEIGSRLGLSPRTIEAHRASMFDKLEVGTLAELIKLFVYAVEAGKAEGMTISPL
                     RSSEAPGDAGTADSTAPASSDDTVA"
     gene            complement(11732..13957)
                     /locus_tag="PKN55_01595"
     CDS             complement(11732..13957)
                     /locus_tag="PKN55_01595"
                     /inference="COORDINATES: protein motif:HMM:NF015859.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNX69968.1"
                     /translation="MATRAISPWRLGVVLWCVGFIIMTLYWLNSEYTRQHALFRQRAS
                     AYAQTVSQRMEQNESVMLSLELLMRTQSQLGMQDVQRFTQQLMQRYPHIYSIQFFEEV
                     SREQAPAFVQRMKQRGLFDFQLKEPATDQPQGWRPVRPRSLYHPIVMIEPQQDGQNSQ
                     LGMDINADGQHSETLRAAEQFGHMESTAPFALPDNGRGYLLVKSVQQHGTRGGEPRQA
                     FVGILLRHDRLLADIPLANSHAHDISVVFYPRVDRAFDNMFFRAPMTPAPAWESTWLP
                     RFHFEQPVSSTAQPFLIKLDYQLRGGDLNLQPLVYLGLTLGMAVGFAMVMLSQRRRSR
                     QQGMEANESLQREREMAAMTLQHIHDGVIRIDSAGRIDYLNPMAATLLQTPPEQVIGQ
                     SVFAVFRLHFEMSQHMQDNPVNESLKFRRPVELPENTSLLRKDGQSLLIEGNISPLPS
                     RQHLHAGALITFRYLGSAHNKIRDRLTTSQKRLREHEEKLAHVARLNTMGEMASGIAH
                     ELNQPLSAIVSYNQACLRMLDDDYPDLELIRSAMHSTANQAHRAGEIITRLRAFVSKQ
                     PARREAISLNQIVRNTLMLAEHALREHGIEILLNLKEDLPPVEGDSIQIEQVTLNLLT
                     NAIDALRHKPQPRQIRLETGATAELACLKVVDNGPGIPDTMLSKLFTPFSTTKPHGMG
                     LGLTICQSIIESHQGHISGRNQPEGGGSFRITLPLQNAQPSSAALCEKEPLSHDANAQ
                     L"
     gene            complement(14287..14946)
                     /locus_tag="PKN55_01600"
     CDS             complement(14287..14946)
                     /locus_tag="PKN55_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012696922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNX69969.1"
                     /translation="MKKTLLAGLAITLAVSGCANTGWDGMSNTQKGAILGTLGGAAAG
                     AAINHKNRGKGALIGAVGGALAGSAVGYYMDQQKRDLDTNLASEVQAGNIIIQQYSDK
                     SIKVTMTSTTAFDTNSYSIKSGFYPTLNKIANTLTKYPKTTLAISGHTDNQGSDAINK
                     PLSENRAMAVSNYLAGKGVIPERMSVVGRGRFEPRASNMTEEGRRLNRRVEILIAPIT
                     E"
     gene            complement(15007..15801)
                     /gene="trpC"
                     /locus_tag="PKN55_01605"
     CDS             complement(15007..15801)
                     /gene="trpC"
                     /locus_tag="PKN55_01605"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014087659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HNX69970.1"
                     /translation="MSDILNTIIATKHREVAAQQALTPLAELAAAARDAAPSRDFVGA
                     IRAKLAAGLPAVIAEVKKASPSKGVIRADFDPAAIAASYAAHGAACLSVLTDRDYFQG
                     CPAYLQAARAACALPVLRKDFIVDPYQIVEARAMGADCILLIAAALSAAQMREFEQQA
                     QEFGLAVLVEVHNAEELDAALTLATPLIGVNNRNLRTFSVSLDTTLDLLPAIGAERIA
                     VTESGIATAEDVTRMRANQVNTFLVGEAFMRQADPGAALSALFGPI"
     gene            complement(15817..16776)
                     /locus_tag="PKN55_01610"
     CDS             complement(15817..16776)
                     /locus_tag="PKN55_01610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012696924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNX69971.1"
                     /translation="MHPSAYRAPAWLPDGHSQTIYPALCLGGARPAYQRERWATPDGG
                     EITVDRLPGRQGMPLLWLLHGLEGGSDSHYARALMRAAQARGWHGVVSHFRGCGGAVN
                     TLPRAYHAGDSAEVAWLLARLASEGRPLCAVGVSLGGNMLLKHLGEAGASAPLCAAAA
                     VSAPLDLAAAGRMLERGLARQLYTRMFLRTLKPASLATARRHPGLIDVRRAQASRTLR
                     EFDDAVTAPLHGFAGVEDYWARASAKPWLRQIARPTLVLNARNDPFLPATALPGPDQA
                     SAAVTLEFPAEGGHVGFASGGFPGRLDWLPQRLLDFFAPHVPH"
     gene            complement(16796..17380)
                     /gene="mog"
                     /locus_tag="PKN55_01615"
     CDS             complement(16796..17380)
                     /gene="mog"
                     /locus_tag="PKN55_01615"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018150050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HNX69972.1"
                     /translation="MNQTLKIGLVSISDRASQGVYEDKGLPALRDWLKRTVTTPLEFE
                     ARLIADEQDEIQSTLIDLVDYQGCQLVLTTGGTGPAPRDVTPEATLAVADRVMPGFGE
                     QMRQISLAFVPTAILSRQVGVIRKQALILNLPGQPKAIAETLEGLRDADGQVKVPGIF
                     AAVPYCLDLIGAPWIDTDPAVVKAFRPKSAQRPA"
     gene            complement(17383..17949)
                     /gene="yjgA"
                     /locus_tag="PKN55_01620"
     CDS             complement(17383..17949)
                     /gene="yjgA"
                     /locus_tag="PKN55_01620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008953600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis factor YjgA"
                     /protein_id="HNX69973.1"
                     /translation="MTTSIQPQQPEDTLISKSQKKREMDALQDLGKTLAGLSTDTLKT
                     MPIPDELREAIREYKRLNAHGALRRQLQYIGKLMRSVDAEPIEARLAVLRGESDQHTG
                     WLHRLERWRDRLLAEDKALDALLAEYPGLDVQTLRTHIRNARREQADNKPPKSYRVIF
                     KMLKDIIPEPGRPAGAPAAQTDEDAHED"
     gene            18052..19398
                     /gene="pmbA"
                     /locus_tag="PKN55_01625"
     CDS             18052..19398
                     /gene="pmbA"
                     /locus_tag="PKN55_01625"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008953601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HNX69974.1"
                     /translation="MTESASSLNYRQAQLADLATQVLDLARRHGATAAEAEVSEGVGQ
                     AVSVRLGEVETIEYTGDKGVGVTVYLGQSKGHASSSDFSAAALEDTVKAAINIARFTA
                     ADPFAGLADPDRLATEFPDLSLCHPWSLSVEEAIELARATEAAGQAVDARVNNSEGAS
                     VNTNLSQFVYANSHGFCHGQQGSRHSLSTTLIAQQDGDMQRDYWYSSARHPQDLLSAA
                     EVGRVAGERAVRRLGARRVKTGQYPVLFEAPLAAGLIGHFVSAVSGGALYRKMSFLVD
                     SLGQQVFSPLVHIDEDPFIPRGIASGAFDNEGVATCARTLVADGVAQGYFLSSYSARK
                     LGMASTGHAGGCHNLLVRDSGEDFAALLQKLGTGLLVTELLGHGVNTVTGDYSRGAAG
                     FWVENGVLAYPVEEITIAGNLKDMFRDIIAIGSDQLVRGATRCGSILLSRMTVAGE"
     gene            19395..20030
                     /locus_tag="PKN55_01630"
     CDS             19395..20030
                     /locus_tag="PKN55_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018747813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2 family
                     protein"
                     /protein_id="HNX69975.1"
                     /translation="MIPADAPGQPAYWRDACAALAADPVMAQLIARYPEAQLSSRGEP
                     FVTLVRAIVGQQISTRAADAVWARLLAAVPQLSPEALLAHAPDALRAVGLSARKVEYA
                     HDLARHFAEGRIDPARFQALDDETLIAELVAVRGIGRWTAEMFLIFNQLRADVWPVDD
                     IGLQRAVSRHYLAGDTPTPKALRAFGQRFAPWRTVATWYLWRSLDPETVLY"
BASE COUNT         3612 a         6133 c         6545 g         3963 t
ORIGIN      
        1 gacacggcgg tgcgcatcac gtccatcggg tggctggcgg ccggcagggc ttccagcgcg
       61 gttttcaccg ccgccggcag gccgcgcagg gccttgagct ttttcttgta gccagtcagt
      121 tcggcctggt tcggcagctt gccgtgcacc agcaggtagg cgatttcttc aaactcgcag
      181 gtggtggcga catcgagaat gtcgtagccg cgatagtgca ggtcgttgcc cgaacggcca
      241 acggtgcaca gcgcggtatt gccggcggcc acgccggaca gggccacgga tttcttcggc
      301 ttgccaacaa caggtgcttc agacatggac aactcctcta tttcgatgtg ggggcggcgc
      361 ggtcagtcgg gggaacgatc cgccgcgcct gttatgaccg gcaataaaac ttacttggct
      421 tctttggcga acagcgcgtc cagcttctgt tcgtactcgt ggtagccaag gtgatcgtac
      481 agatccatgc gggtttgcat ggtcggcacc acattctttt gcgtgccttc ctggcgcagg
      541 gtctggtaga cattcagcgc agccaggttc atggcgcgga aagccgacag cgggtacagc
      601 gccagcgaca cgcccaccga ggccagctct tcggtggtga acagcggggt ggagccaaat
      661 tcggtgatgt tggccagcac cggcaccttc accgcctcaa caaactgctt gtacatggcc
      721 agttcggtca tggcttccgg gaagatcatg tcggcgccgg cttccacgca ggcgcaagcg
      781 cggtcgatgg cggcttgcag accgtccacc gccagcgcgt cggtgcgcgc catgatgaca
      841 aagtcgttgt cgcggcgggc gtccacggcg gccttcacgc ggtccaccat ttcatccagc
      901 ggcacgatgg ccttgttcgg gcggtggccg cagcgctttt gctgcacctg gtcttcgatg
      961 tgcacggcgg cggcgccggc tttttccaga tggcgaatgg cgcgggcgat attgaaagcg
     1021 ccgccccagc cggtgtcgat atccaccagc agcggcagtt cggtcacgtc ggtgatgcgg
     1081 cgcacgtcga tcagcacgtc ttccagggtg gtgatgccca ggtcaggaat gccgcaggag
     1141 ccggccgcca cgccgccgcc ggacaggtac agcgccttgt agccgctatg gtcggccaag
     1201 cgcgcggcgt aggcattgat tgcgcccacc acctgcaagg gtttttccgc ggccacagcg
     1261 gccttgaaac gttgtccggg ggtcatcatg tgccgtttct cctctaggtg ttctgggctt
     1321 gatgaacggc attctagcaa gtgcttggtt ggggtgtaaa tacaaaaatt cgtctcggtg
     1381 gctgacaagg aaaaacacac gcacctgacc gctgggcaat ccccctgaac agtctgagag
     1441 agatgcgtcg atggcaaata aaaccacata aaatcaattg gatttgaatt tttctggcag
     1501 accttgcgtg gcgagcagct tgacggtgcg ctggcttctg gcagcggcct tacggttgca
     1561 ccagatgcca tcgttatcta gaatctggcc gaaaaatgcc attttggcag gatgtgtgac
     1621 aggcgctgaa tttaagagcc gcccacaaaa cccagcgcag cgatcctgac aaggtgcgcg
     1681 accgcagaca tgctaaggcc gggccaccac gccagagggt ttgctgagcg gcgctcaata
     1741 cgcagaggtg agtgtgttga tcttatggaa tttgtttcac tggggtagtg cagccttcat
     1801 tattgttgtg ctgttttttg cttttattgg cccgccaaac aaacaaggca caccatatac
     1861 cgtggcagtg caaccacaag ctgcgcctgc caatgcaccg gcagagcaca gcaaaaagtt
     1921 taatttctga gacaccgaat atgcatatgg cgtgcagcaa catgattttc atcagcaagc
     1981 agcttgttgg ctgtttgttg gctttcagct tggcatgcct gggtacgacc gcccaagcgg
     2041 ctgagtgcaa tggcctgaaa gttgccactg gccctgctgg taaagggtac gcccgactgt
     2101 ttgccgatat gcaacaggtg tgtggtcgga cggtggcgat gtgtgagttg ccgacgtctg
     2161 gcggtctgga caatctgcac gccatgtctg ccaatgaggc ggatattggt tttgcccaag
     2221 tagacacatg ggctgatatg caaatgagca atgaaaatat tgccggcttg caagcggtgc
     2281 tatcgctcaa tttcaattat ctgcatgtgg tggtggctgc caacgggttt gctgtggcgg
     2341 gagaaagccg ctttggtggc ttgttgcgcg gcaagccaca atatgtgttg atggagcgtt
     2401 tcagcagttt gcgtggtcag cgcgtggcgg tggtgggatc gaccgcgtta ttggggcgca
     2461 agctggagca acgcttgcgt tatggcctgc actttgtcga tgttgagaca gatgatcagg
     2521 cgttccagct tgtcacctct gacaaggtgg ccgctgcttt gacggtatca ggctggccga
     2581 gcggcacggt gggagcgcta aagcccgacg atcgcctgag cctggcgcct tttgatgccc
     2641 ccagtgatgc gccctatgtg gtgcgccccg tcagctaccg ggggcttggg gtatataata
     2701 ccaatatgct ggccgcgccg aatgtcttgc tgagccggcc attcaaaggc gcgaaagcca
     2761 gccaggttgc ggcgctgaag gcctgtgtgg ccagccacat gctggagctt caagaaggca
     2821 gttattcgcc aggctggaaa gaaatcaagt ctctggataa aacctggggc attccgccat
     2881 ttaagggtgc ggttgcccca gcaaaaactg accggaaaaa ataagccgcg ccaacaaacg
     2941 cttcttcttg gcgtggtggt gtgaccttgc ccgactcccg cttacaccct ttggtcacac
     3001 acctcaccca gcacgccagt gccggaatat tgggcgctgg cttgatcaat catcagccgc
     3061 ccaagccatt caccaccccc gccaccacgc ccgccgccac cagcgcgcac agcacggcca
     3121 gtttccatga ggcaaagcgc agctcgggcg gttggctggc gtaggtttgc cagccgctga
     3181 gcatggcgac caccagattg cggcgcttga tcaggcgata gaacacaatg gcgctcaggt
     3241 gcaggccaac ggcggccagc agcagtttga acgaaatttt gtgccagcca gtgatggcct
     3301 ctgacagatc gctattgatc agcttggcca gcgggccatc ccacaagaac gaatcgacat
     3361 cagtggcaaa caggccgctg cccacctgaa aacccagtaa acccagcagc gccagcacca
     3421 tcagcgcgcc cagcgggttg tggccgggga tgcggtgttc cggcaggctg ccgcgcagat
     3481 aggccagcat ggcgccgggg ccgcgcacaa agcgggcaaa gcgggcggtg tcgctgccca
     3541 acacgcccca cagcaggcga aacagcagca ggccggcaat gccatagcca gccagcaggt
     3601 gaaggcgcaa ggcatcgccg ccggtttcac cggaccacca catcaccccg aacaagagca
     3661 ccaggctcca gtgaaacagg cgggtggggg tatcccacag tttgattcgg tattgcatga
     3721 cagtttctcc acgaaagccc aacagactgg cgtcatggcg tttgggttcc gagacggagc
     3781 ccatccgtcc ggcggatgtg ggccggacgg gggccggttc aggcggccag cgcctgggtg
     3841 cgctcagcca gggcggccag ggtgcgctgg gctgcaccgc tgtcgatggc ctggcgggcg
     3901 cggtcgatgg cctcgctgag ggtggcggcc acgccggcca catagatggc ggcgccagcg
     3961 ttcagcagca cgatatcgcg gcagggaccg gtttcgccgg ccagcacagc gcgcacgcgg
     4021 gtggccgatt cttcggcgct gctggctttc agggcgctga tatcgcaata gttcaggccg
     4081 aattcacgcg ggtccaacag gtattcgctg accgcgccgt ctttcagctc ggctacgcgg
     4141 gtgcggccgg tgagggtgat ttcgtccagg ccgtcttcgc cgtgcaccac cagcacatgg
     4201 cggctgccca actggcgcag cacccgcgcc tgaataccga ccagatccgg gtgaaacacc
     4261 cccatcacct gattatcagc gccggccggg ttggtcagcg ggccgaggat attgaacagc
     4321 gtgcgcacgc ccagttcgcg gcgcaccggg gcgacgtgct tcatggcact gtgatggttg
     4381 ggagcaaaca taaaacccag gccaatgtcg tcgatgcatt ggcccaccgc ctcggcgctg
     4441 aggttgagct tgacgcccag ggtttccagc acgtcggcgc tgcccgagct ggacgacacc
     4501 gagcgcccgc catgcttggc cacccgcgcg ccagcggcgg cggcgacaaa ggccgaggtg
     4561 gtggaaatat taaaagtatg cgcgccatca ccgccggtgc cgcaggtgtc taccagatgc
     4621 tcgcgctttg ccactggcac cggggtggcg aattcgcgca tcaccgtggc ggcagcggcg
     4681 atttccgaga cactttctac tttcacccgc aggccgatca ggatggcggc aatctgcgcc
     4741 ggggtgagtt ccccgcgcat gatctggcgc atcaggtcca gcatttcgtc gtaaaacagt
     4801 tcgttgccgt caatcaggcg atttaacgcg gcttgggcgg aaatcatcaa aggctctcta
     4861 tcgcaatgga aaatggcagg tcagtccgtg acataggccc ggcagcgcgg ggccatcggc
     4921 atcaagcgtg gctaaaaaac ccagtgccgc ggtcctggcc aggcgcacgg ccgcagacag
     4981 ttaaagatgt cgtgcaaggc caggagggtt ttgttggccg ctctaggcgt gggcggtcag
     5041 gaagttgcgc agcatggtgt gaccatgctg ggtcaggatg gattccgggt gaaactgcac
     5101 gccctcgacc gccagggttt tgtggcgcac gcccatgatt tcgccgtctt ctgtccaggc
     5161 ggtgatttcc aggcaatccg gcaggctttc gcgctcgatc accagcgagt ggtagcgggt
     5221 gcaggtcacc gggttgggca gactgtgaaa cacgccgagg tccagatgct gcactggcga
     5281 ggttttgccg tgcatcagtt gcttggcgtg gatgatcttg ccgccaaacg cctggccgat
     5341 gctctggtgg cccagacaca cgccgaggat gggaatctgg ccggcaaagc gctcgatggc
     5401 ggccaccgac acaccggctt ccagcggcgt gcacgggccg ggggaaatca ccaggtattg
     5461 cggcgccagg cgggcgatat cgtccaggct gatttcgtcg ttacggaaca cctgcacgtc
     5521 ttgccccagt tcgccgaagt actgcaccag gttgtaggtg aaggagtcat aattgtcgat
     5581 catcagcagc atgtttggcg tatcccattg atttatctgg ctaagtgatt ttttaaagtc
     5641 tatgcatacc cgcttgaacc cgctgtctgt cgggtgggcc agggcttttg gcggtgacaa
     5701 acaagtatta tctccagtgc agcgccgggc ggcaagaaat gcaaaaggag cccatcggcc
     5761 ccttttgtag caacccctcg ctgtgcgcca gccggctcag ccatcaatca gcttggccag
     5821 ctcgccatag gcaacggtat ctttattggt ggtcatcagt ttgaggaagc gcgtcaggtt
     5881 gccattacac agatgcagaa tgaccacctg ggccagttgg cgcgccagtt gttgactggc
     5941 ggtgagctcg ctttgcccat tacgccccag ttcggccaga tccagcaagg tgcaggcctg
     6001 gccgtagtag ctgttttgag tgggagacac cttcagcacg agccacttta ccccgctgta
     6061 gacggtggtc agcaggccga acggggtcac ctcaaatgct gccttggccg cggtccagtc
     6121 gatggagtcc aggcaggcat tcaggcaggt ttccagcttt ttcagatcgg ggtcggcatc
     6181 ggatttcagt gtttggcgag cctgctcgcg agcactgaac agcggcccct gttttttaac
     6241 cagattggaa aactgataag tggctttcca tggcgccagt acattgctgc caaaagtcac
     6301 cgccttgccg ctgaacccca gtaccgagta accgatcagc cccacggtct gcccggctgc
     6361 cgcattcagc ccgcggttga tcagcgaaga aatcaccttg ccggcaatca gtccgggcag
     6421 ggcgtcgcgc agcaaggcca gtaaaccatc tgccggcagg tctttcatct gcacggggct
     6481 tctgttgtca agagcagcca attgtttgct gaaccaggtt tcccagtcac tattgccggt
     6541 ctgccgtgac atgctggaca gcagcggcca tagcggctgg caaatgctgt ccagcgccac
     6601 cagatctgcc ggcaaggtgg cttgttgcaa cagcgtgggc ggtgacatgc gcgacaccat
     6661 cacgtcattg tcttttcggg gcaaaaatcc gtcagtggca cgcacaaatg cggccagccg
     6721 tgccgacaga caggcccccg actgggtatt gaacaagtcc tctaccgggc cacaggcctg
     6781 ctggtattgc tgaggtgatg ccggctcgcc atcatcacca ttggtttggt aagcatccca
     6841 tgcccacccc aggtgggtgc cagcctcctg ctggcgcacc aaggcatagc agcttggatc
     6901 caccagcaaa aactggatat tgtcgcgaaa ctgcggcatg ccccagtcac tgctgacccc
     6961 aggtacatcc cgagccgggc ccagtttgtt aaaagccagc cggccgctca ggacttggtc
     7021 aaacgtggca caagccgggc aactcagcgt ctggaacgtg gtctccttgg ccggcgcgga
     7081 caaggcccgc tccaggctgc tgattatggc attttcatcg ggcgggctca ccgtattgcc
     7141 gggcacgtca cggacaaagc gggccaggcg ttcgggcaga ttcaggttgt cgtcaacatc
     7201 aaacaggatt tccgccgggc cgcgatagcc ggcggtgttc tcactcttgt aggcgtccct
     7261 ggcccgttcc agcgcgcccc tggcgccggc cagctcaacg gcggcaagca gattggcctc
     7321 gctggtcata tagctcagat tgtcgcggat ttgcgggtcg ccccagcagt cgtgtttctg
     7381 ggctttccgg gcattgttca atagtgtgtc aaatgtctgg catgctggcg ccgaaatcac
     7441 tttcagcgtg gtcgagggag gcataatgat ctcctgagca aaataatggc cgcacacaca
     7501 ctggcggccc ggcagcaata acgcagcggg cgccctggtg tgaagcgggc agtctgccgg
     7561 tgcagcatcc catggtgaaa tggcatgttg catcggtttg cccggtaaat ctgttatgct
     7621 cattggccat gcatgtcatt tcttttcccc gcttaatctg ccgtctctgc ctgatcctgc
     7681 tgctggcgcc ctgcctgttg ctggcccggc cggcgtgggc attgacaccg gtcaccctgc
     7741 aactgaagtg gacgcatacc ttccagtttg ccggttacta tgcggcacaa atacgtggct
     7801 actaccgcga agccggcctg gatgtcaccc tgcgtgaggc ccagcccggc gatgatccct
     7861 tgcgtgccgt actgtctggc cgtgccgaat ttggcgtcgg caccagcagc ctgctgctgg
     7921 cccgtcaccg gggtgcgccg gtagtggtgc tggcggtgat ttttcagcac tcacccctgg
     7981 tattgctgag caaatccggc gggccttccg ctggcattca cgacttgctg gacaagcggg
     8041 tgatgctgga gccgcaatcc gacgagctgc tggcctactt gcagcaagag ggcatttctt
     8101 cacggcgcat tacctggctg gcacactctt accgccccac ggccttgctt gactccggcg
     8161 tggatgcggt ttctgcttac tcgatcaatg agccgtatta tctggatcag gccgggctgc
     8221 agtatcagac cttcaccccg cgttcggccg gcattgattt ttatggcgat aatctgttta
     8281 cccgcgaagc cttgctgaac gattctcccg agatggtacg ggcattccgg acggccagct
     8341 tacgcggctg gcagtacgcc atggcccatc cggaagaaat catccagtgg ctggtcaaca
     8401 cctatcctgg ccgccaccca gctgattttc atcgccacga agcccggcgc atggcgccgc
     8461 tgctgcgcac ggatctggta gaagttggct atatgaaccc ggggcgctgg cggcatattg
     8521 ccgacactta cgccgagctg ggcctgctgc cgagccagtt ttcactgaaa ggttttttat
     8581 atcagaccaa acctgccatc gactggctct ggctgggcgg tgcactggcc gtgacggcca
     8641 tgattggcgg cgtggccctg tacattcacc gcatcaaccg ccagctgacc cgcgcgctga
     8701 ccaccagtca ggccaccctg aatgccctgt atgccagtga ggcacggttg cgcgagctga
     8761 atgcccgtga cgccctcacc ggcctggcca atcgtgaacg cctgttcgag caactggaca
     8821 ccctgctggg cgcgccacag gcgcaactgg cggtattgtt tattgatctg gatggcttca
     8881 aggcgattaa cgaccaattt ggccacgctg cgggcgacca actgctgcgg gtgatggccg
     8941 agcgcatgcg cgccagcctg cccgcccggg ccttgctggc gcggctgggg ggcgatgagt
     9001 ttgtggccgt actacccgag gccgcgcacg cgaccgaaca cgcccagcag ctgctggccg
     9061 cgctggccgc cccactgccc acctgtgact gcaccctgtc ggccagcatc ggcatggcct
     9121 gtgcgccaac cgacggccac gacgcgcaaa ccctggtgca acacgccgat ctggccatgt
     9181 atgccgccaa gcgcaatggc cgcaatcagc tgcaacgctg gccggtggcc gccttggcca
     9241 gggactgagc cgcacgcgct gccattgccg cgccagtctg gccgccgtat gctgtcagcc
     9301 ttgttgcctg actgtgttcc gcccatgaaa aacatcgccg atatccgcaa agactacgcc
     9361 agccaaacgc tggaccctga cgactgcctg gcccacccgg tggcgcaatt cagccgctgg
     9421 ctggatgacg ccatcgccgc cgacgtgccc gaacccaccg ccatgctggt gtccaccgtc
     9481 tcgcccgaag gtcgcccggc cgcgcgcgtg gtgctgctca agggcgtgga agacaatcaa
     9541 ctggtgttct acaccaatta ccacagccgc aagggccagc acctgggcca tacaccgttt
     9601 attgcgctga cttttttctg gcccgagctg gagcggcagg tgaatatcga aggcgtggtc
     9661 aatcgcgtgg cagacgaaac ctccgacgcc tattttgaca gccgccccta caaaagccgg
     9721 gtgggcgcct gggcatccga gcaaagccag ccgatcagcg gcaaagaagt ggtgatggcg
     9781 cgctttgccc gctttgccgc gcaatacctc acccatgtgc cgcgcccgcc gcactggggc
     9841 ggctttgccg tcacccccga gcgcatggaa ttctggcaag gccggcccag ccgcctgcac
     9901 gaccgtgtgc tctacctgcc cgatggccag ggcggctggc aaaagcaaag gctggcgccg
     9961 taaatccacc cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ngcataaatt
    10081 cgtcaaaccc gctgattttg acaatcacgc cgctgtgttt ggcgatggtg gccagcacat
    10141 tctgggcggg gggctcatcg cggtagcacc agaacaggcg gccagggatg tggccagcgg
    10201 gcaggtcgtt caaaaagccc atcaggctgc cgtcgttgcc gccgtagccg agcaccaggg
    10261 gggtggagta ttggaatagt tgcgacaggc tgttgcccca gccttgtccc agggtgttga
    10321 cgccttctcg gtcgtttttt ggggccaagt gcaggtcgcg gtgaattttg gccaccagcg
    10381 ggcggcgcag ttgcgggcgg gcaaagccgg ccagggcttc gtggccgaca atcagtggct
    10441 ggcggcgcgc atgaatggcc agtgcatcgg ccaccagatt gtcaaaattg gtgctcacca
    10501 ccacgcgatg gtgagtttca tccagaattt tggccaggat ggaatagccg acgctggggg
    10561 cggcgtcttc catggcgtct tccagcgcgg catagcccga ggtgtaatcc ccggcaaaac
    10621 ggcgctcaaa gatggttgaa taatgactgg cggcgtcttg ataggctggc ttggcgtctt
    10681 gatgtgtgct gaatgttggg ttttccagct gctcgcgcgc ccagctttgc aggtcggtat
    10741 cgggctccag gcattcgcgc tgatgccatt cttgcagcca gtcataggct aatttgccgc
    10801 cagtgagaat cccggacgag gcggaagccc cagcgcccag aataaaggcc agcgagcggc
    10861 cttgttgatg taattttcgt atttccgcca gcaggccttt ttcagaccat tcttttgggc
    10921 tgtgttcacc attcgtcatc tgggtgtctc ttgcaaatca accaaaaacg gcctgccacc
    10981 ccaccgggca acaggccgtt tgtctcagca catcacgctt gctcaggcga cggtatcgtc
    11041 gctgctggcg ggggcggtgc tgtccgcggt gccagcatcg cctggcgctt cgctgctgcg
    11101 cagcggggag atggtcatgc cttcggcctt gcccgcttcc acggcataga caaacagttt
    11161 gatcagttcg gccagggtgc cgacttccag tttgtcgaac atgctggcgc ggtgggcttc
    11221 gatggtgcgc ggcgacaggc ccaggcggct gccgatttct ttgctggaca agccttcggt
    11281 gaccagttgc agcacttcca gctcgcggct ggacaggcgg gccaggcgca tttcggcttc
    11341 ttgggtgcgt gcggtttttt cgtgggtctg gatgcagtgg cgcagcgcct tgcgcacggc
    11401 ttccatcagc agttcgccgt cgatcggctt ggtgagaaag tcgctggcgc cggccttgaa
    11461 ggcgcgggtg cactgcgcca cgtcgccgtg gccggtgatg aagatgatgg gcagggccac
    11521 gccggcgcct tcgagttcgg cttgcagttc caggccggtg gtgccaggca tgcggatgtc
    11581 cagcaccagg caggcgatct gattggggat gaacgcggcc agaaactcca gcgcggacgg
    11641 gaaggccatc actttcaggc cggcgctgcg gatcagcagg ctgaccgaat ccagcacggc
    11701 cggatcgtcg tcaaccacat acacaatcgg gtcaaagttg ggcgttggcg tcatgagaca
    11761 aaggttcctt ttcacacagt gcagccgagc ttggctgcgc gttttgcagc ggcagggtaa
    11821 tgcggaaact gccgccgcct tcgggctggt tgcgcccgga gatatggcct tgatgcgatt
    11881 cgatgatcga ctggcagata gtcagcccca aacccatgcc atgtggcttg gtggtgctga
    11941 acggggtaaa cagtttgctg agcatggtgt cgggaatgcc cgggccattg tccaccactt
    12001 tcaggcaggc cagttcggcg gtggcgccgg tttccaggcg gatttgccgt ggttgtggct
    12061 tgtgcctgag cgcgtcgatg gcattggtca gcaggttgag ggtgacttgc tcgatctgga
    12121 tgctgtcgcc ctccactggc ggcaggtctt ccttgagatt gagcaggatt tcaatgccat
    12181 gttcgcgcaa ggcgtgctcg gccagcatca gcgtgttgcg cacgatctga ttcaggctga
    12241 tggcttcgcg ccgggccggc tgtttggaga caaacgcacg cagccgggtg atgatttcgc
    12301 ctgcccgatg tgcctggttg gcggtgctgt gcatggcgct gcggatcagc tccagatcgg
    12361 gatagtcgtc gtccagcata cgcaggcagg cctggttgta gctgacgatg gccgacagcg
    12421 gctggttgag ctcgtgggca atgccggagg ccatttcacc catggtgttg agccgggcca
    12481 catgcgccag tttttcttcg tgctcgcgca ggcgcttctg gctggtggtg agccggtcgc
    12541 gaattttatt gtgggcactg cccagatagc gaaaggtgat cagcgcgccg gcgtgcaggt
    12601 gctggcgtga gggcagcggc gagatattgc cctcgatcaa caggctttgc ccgtccttgc
    12661 gcagcaagga ggtgttttcc ggcagttcga ccgggcggcg gaatttcagg ctttcgttga
    12721 ccgggttgtc ctgcatatgt tgcgacattt caaaatgcag acgaaatacg gcaaacaccg
    12781 actggccaat cacttgctcg ggaggtgttt gcagcagcgt ggcagccatc gggttgagat
    12841 agtcgatgcg cccggcgctg tcgatgcgga tcacgccgtc gtggatgtgc tgcaaggtca
    12901 tggcggccat ttcgcgttcg cgttgcagcg attcgttggc ttccatgcct tgctggcgcg
    12961 agcggcggcg ctggctgagc atcaccatgg caaaacccac cgccatgccc agagtcaggc
    13021 ccagatagac cagcggctgc aggttgaggt cgccgccgcg cagttgataa tccagcttga
    13081 tcaaaaacgg ctgggcggtg ctgctgacgg gttgttcgaa gtgaaagcgc ggcagccagg
    13141 tgctttccca ggccggggcg ggcgtcatcg gcgcgcggaa aaacatattg tcaaaagccc
    13201 gatccacccg tgggtaaaac accacgctga tgtcgtgggc gtggctattg gccagcggaa
    13261 tatccgccag cagccggtcg tggcgcaaca gaatgccgac aaaagcctgg cgcggttcac
    13321 caccgcgtgt gccatgctgt tgcacggact tgaccagcaa ataaccacgg ccattgtcgg
    13381 gcagggcaaa tggcgcggtg gattccatgt gaccaaattg ctcggcggcg cgcagggttt
    13441 cgctgtgctg gccatcggca ttgatgtcca tgcccaactg gctgttctgg ccatcctgct
    13501 ggggttcgat catcacgatg ggatggtaga ggctgcgcgg acgcactggc cgccatcctt
    13561 gcggctggtc agttgccggc tctttgagct gaaaatcaaa aagcccgcgt tgcttcatgc
    13621 gctgaacgaa ggccggggcc tgctcgcgcg aaacctcttc aaaaaactgg atgctgtaaa
    13681 tatgcggata gcgctgcatc aactgctggg taaagcgctg cacgtcctgc atgcccagtt
    13741 gtgactgggt gcgcatcaag agctccagcg acagcatcac cgactcgttt tgttccatgc
    13801 gctgcgacac ggtttgcgca taggcgctgg cacgctggcg gaacaaggca tgctggcggg
    13861 tgtactcgct gttcagccag tacaaggtca tgatgatgaa accgacgcac cacagcacca
    13921 cgcccagccg ccaagggctg attgcgcggg ttgccatatg tgggccagca aggggtttca
    13981 tccggtcagc gcattcatca gaaaggagtt tgccgggctg taccgcccgg tcaggccaac
    14041 agcatgccag ccaggcaaaa gccttgctgg tgctgagtgg atcacttcag tatgttggca
    14101 tgtctgcggc catgccacaa gggggaatcc accaacatgg gcatgaagtt tcccgatggc
    14161 ggacaaatgt gcaagatagg tgtcagcaat gcccacgcag aatgtgggca ggcacaccgg
    14221 gcacgacaca gctggacgct ttcatgctgt cgtcacccgg tttactggac agaaatgcct
    14281 taaggattat tcggtaatcg gcgcaatcag gatttccacc cgacgattca gccggcgacc
    14341 ttcttcggtc atattgctgg cgcgcggctc aaagcggcca cgacccacca cgctcatgcg
    14401 ctccggaatc acgcccttgc ccgccagata attggacact gccatggcgc ggttttctga
    14461 cagcggcttg ttgatggcgt cgctgccctg attgtcggtg tggccagaaa tcgccagggt
    14521 ggttttcggg tatttggtca gcgtgttggc gatcttgttc agcgtcggat aaaagccaga
    14581 cttgatgctg tagctgttgg tatcaaacgc ggtggtcgaa gtcatggtga ccttgatcga
    14641 cttgtcgctg tattgctgga tgatgatatt gccggcctgc acttcgctgg ccaggttggt
    14701 atccagatca cgcttttgct gatccatata gtagccgaca gccgagccgg ccagcgcgcc
    14761 gcccacggcg ccgatcagcg cgcccttgcc acggtttttg tggttgatcg ccgcgccagc
    14821 ggccgcgccg cccagggtgc cgaggatggc gcccttttgc gtattgctca tgccgtccca
    14881 gccggtgttg gcgcagccgc tgacagccag ggtgatggcc agtccggcca gcagggtttt
    14941 tttcatggtg gtgtctccat gtgggccggc gccagggccg gcaaattcag taagctggtg
    15001 aaagcgtcag atcgggccaa acagcgcgct gagcgccgcg ccggggtcgg cctggcgcat
    15061 aaaggcctcg cccaccaaaa aggtgttgac ctggttggcg cgcatgcggg tcacgtcctc
    15121 ggccgtggcg atgccgcttt cggtaacggc aatgcgttcg gcgccgatgg ccggcagcag
    15181 gtccagcgtg gtgtccaggc tgacgctgaa ggtgcgcaga ttgcggttgt tcacgccaat
    15241 cagcggggtg gccagggtca aggcggcgtc cagctcttca gcattgtgca cttccaccaa
    15301 gaccgccagg ccaaattctt gggcctgttg ctcaaactca cgcatttgcg cggcgcttaa
    15361 ggcggcggcg atcagcagga tgcaatcggc gcccatggcg cgggcttcga cgatctgata
    15421 cgggtcaaca ataaaatcct tgcgcagcac cggcagcgcg caggcggcgc gggcggcttg
    15481 cagataggcc gggcagccct gaaagtaatc gcggtcggtc agcaccgaca ggcaggcggc
    15541 gccgtgggcg gcgtagctgg cggcgatggc ggccgggtcg aagtcagcgc gaatcacgcc
    15601 cttgctcggg ctggcttttt tcacttcggc aatcaccgcc ggcaggccgg cggccagctt
    15661 ggcgcggatg gcgcccacaa aatcgcggct gggcgcggcg tcgcgcgcgg cggcggccag
    15721 ttccgccagc ggggtgagcg cttgctgggc ggccacttcg cggtgtttgg tggcgatgat
    15781 ggtgttcaga atatcggaca tgagcaaagc atccagtcag tgcggaacat ggggggcaaa
    15841 aaaatccagc aggcgctgcg gcagccagtc cagccggccg ggaaagccgc cgctggcaaa
    15901 gccgacatgg ccgccttcgg cggggaattc cagcgtcacc gccgcgctgg cttggtcggg
    15961 gccgggcagc gctgtggccg gcaaaaacgg gtcgttgcgg gcgttgagca ccagggtggg
    16021 ccgggcaatc tggcgcagcc agggcttggc cgaggcgcgc gcccaataat cttctacgcc
    16081 ggcaaagccg tgcagcggcg cggtgacggc gtcgtcaaac tcgcgcagcg tgcggctggc
    16141 ctgcgcgcgg cgcacgtcaa tcaggccggg atgccggcgc gcagtggcca ggctggccgg
    16201 cttgagcgtg cgcaaaaaca tgcgggtgta cagctggcgc gccaggccgc gctccagcat
    16261 gcggccggcg gcggccaaat ccagcggggc ggacaccgcc gccgccgcac acagcggcgc
    16321 gctggcgccg gcctcgccca ggtgcttgag cagcatattg ccgcccagcg acacccccac
    16381 cgcgcacagc ggccggcctt cgctggccag tcgcgccagc agccaggcca cttcggcgga
    16441 gtcgcccgcg tggtaggcgc gcggcagggt gttcaccgcg ccgccgcagc cgcgaaaatg
    16501 gctgaccacg ccatgccagc cgcgcgcctg tgcggcgcgc atcagcgcgc gggcgtaatg
    16561 gctgtcgctg ccgccttcca ggccgtgcag cagccacagc agcggcatgc cctggcggcc
    16621 gggcaggcgg tccacggtga tttcgccgcc atccggcgtg gcccagcgct cgcgctggta
    16681 ggccggccgc gcgccgccca ggcacagcgc cgggtagatg gtctggctgt ggccgtcggg
    16741 cagccaggcc ggcgcgcggt aggcggacgg gtgcagcgtg gcgctcaccg tggcctcagg
    16801 ccgggcgctg ggcggatttc gggcggaagg ctttcaccac cgccgggtcg gtgtcgatcc
    16861 acggcgcgcc gatcaaatcc aggcagtagg gcacggcggc aaaaatgccg ggcactttca
    16921 cctggccgtc agcgtcgcgc aggccttcca gcgtttcggc aatcgccttg ggctggccgg
    16981 gcaggttcag aatcagcgcc tgtttgcgga tcacccccac ctggcgcgac aaaatcgccg
    17041 tcggcacaaa ggccaggctg atctggcgca tttgctcgcc aaaaccgggc atcacccggt
    17101 cggccaccgc cagggtggct tccggggtga cgtcgcgcgg cgccgggccg gtgccgcccg
    17161 tggtcagcac cagttggcag ccttggtagt ccaccaggtc aatcagggtg gattggatct
    17221 cgtcctgctc gtcggcaatc aggcgggctt caaattccag cggcgtggtc accgtgcgct
    17281 tgagccagtc gcgcagcgcc ggcaggcctt tgtcttcgta cacgccctgg ctggcgcggt
    17341 cgctgatcga caccaggccg attttgagtg tctggttcat gctcagtcct cgtgcgcgtc
    17401 ctcatccgtc tgagcggccg gcgcgccggc agggcggccc ggctcgggga tgatgtcttt
    17461 gagcatcttg aaaatcaccc ggtaagattt gggcggcttg ttgtcggcct gttcgcggcg
    17521 ggcgttgcgg atatgggtgc gcagggtttg cacgtccagg ccggggtatt cggccagcag
    17581 cgcgtccagg gctttgtctt ccgccagcag gcggtcgcgc cagcgctcca gccggtgcag
    17641 ccagccggtg tgttggtcgg attcgccgcg cagcaccgcc agccgggcct cgatcggctc
    17701 ggcgtccacg ctgcgcatca gcttgccgat gtattgcagt tgccggcgca gcgcgccgtg
    17761 cgcgttcagg cgcttgtatt cgcggatggc ttcacgcagc tcgtccggga tgggcatggt
    17821 tttcagcgtg tcggtggata agccggcgag ggttttgccc aggtcttgca gggcgtccat
    17881 ctcgcgcttt ttttgcgact tgctgatcag cgtgtcttct ggctgttggg gttggatcga
    17941 agtggtcatg gcgcttgggg aaaaaactaa gactggtatg atacaggaag cctgcccgct
    18001 cttgcggctg acgcgcttgc gggtgttttt catttcattg tgactgtgac catgaccgaa
    18061 tccgccagct ccttgaatta ccgccaagcc caactcgccg accttgccac ccaggtgctg
    18121 gatctggccc gccgccatgg cgccaccgct gccgaggcgg aagtgtccga aggcgtcggc
    18181 caggccgtgt cggtgcggct gggtgaagtg gaaaccatcg aatacaccgg cgacaaaggc
    18241 gtgggcgtca ccgtgtatct gggccagagc aagggccacg ccagcagctc ggatttttct
    18301 gccgccgcgc tggaagacac ggtgaaagcc gccatcaata tcgcccgctt caccgccgcc
    18361 gacccgtttg ccggcctggc cgacccggat cggctggcga ccgaattccc ggatttgtcg
    18421 ctgtgtcatc cgtggtcgct gtccgtcgaa gaggccatcg agctggcgcg cgccaccgag
    18481 gcggccggcc aggcggtgga cgcgcgggtg aacaactccg aaggcgccag cgtcaacacc
    18541 aacctcagcc agtttgtcta cgccaacagc cacggctttt gccacggcca gcaaggcagc
    18601 cgccacagcc tgtccaccac gctgatcgcc cagcaggatg gcgatatgca gcgcgactac
    18661 tggtatagca gcgcccgcca cccgcaagat ttgctcagcg ccgccgaagt gggccgggtg
    18721 gccggcgagc gcgcggtgcg ccgcctgggc gcgcgccggg tgaaaaccgg ccagtatccg
    18781 gtgctgttcg aagcgccgct ggccgccggg ctgattggcc attttgtctc ggcggtcagc
    18841 ggcggcgcgc tgtaccgcaa aatgagcttt ttggtggatt cgctcggcca gcaggtgttt
    18901 tcgccgctgg tgcacatcga cgaagacccg ttcatcccgc gcggcatcgc ctctggcgcg
    18961 tttgacaacg aaggcgtggc cacctgcgcg cgcaccttgg tggccgatgg cgtggcgcag
    19021 gggtatttcc tgtcgtcgta ctcggcgcgc aagctgggca tggccagcac cggccacgcc
    19081 ggcggctgtc acaacctgct ggtgcgcgac agcggcgaag actttgccgc cctgctgcaa
    19141 aaattgggca ccggcttgct ggtcaccgag ctgctgggcc acggcgtgaa caccgtcacc
    19201 ggcgattact cgcgcggcgc ggcgggcttt tgggtggaaa acggcgtgct ggcctacccg
    19261 gtggaagaaa tcaccatcgc cggcaacctc aaggacatgt tccgcgacat catcgccatc
    19321 ggcagcgacc aactggtgcg cggcgccacc cgctgcggct ccatcctgct gtcgcgcatg
    19381 acggtggccg gcgaatgatc ccggccgacg cgccggggca gccggcctac tggcgcgacg
    19441 cctgcgccgc gctggcggcc gacccggtga tggcgcagct gatcgcccgc tatcccgagg
    19501 cgcagctgag ctcgcgcggc gagccgtttg tcaccctggt gcgggcgatt gtcggccagc
    19561 agatttccac ccgcgccgcc gatgcggtgt gggcgcggct cttggccgcc gtgccgcagc
    19621 tatcgcccga ggcgctgctg gcccacgcgc cggacgcgct gcgcgcggtg gggctgtcgg
    19681 cgcgcaaggt ggaatacgcc cacgacctgg cccggcattt tgccgaaggg cgcatcgacc
    19741 cggcgcgctt tcaggcgctg gacgacgaaa ccctgattgc cgagctggtc gccgtgcgcg
    19801 gcatcggccg ctggacggcc gaaatgtttt tgatcttcaa ccaattgcgc gccgatgtct
    19861 ggccggtgga cgacatcggc ctgcaacgcg cagtcagccg ccattacctg gccggcgaca
    19921 cccccacgcc aaaagccctg cgcgcctttg gccagcgctt tgcgccgtgg cgcacggtgg
    19981 ccacctggta tttgtggcgc agcctggacc cagagacggt gttgtattaa gcggtgcgtg
    20041 gggtccgcct gacgttgttg cgcggccttg ccgtgtccgc acacaccgca gtactgagtt
    20101 gtgcaagctg ggccaagcca ccacagcgac cgtctgtggc cagaaaaggg cgatgacttc
    20161 gtctttgttt ctttgcataa tcatcctccc agcgtgcgtg atcacgcatc acacgggaac
    20221 ccagggagac ggatttcact cccaatccag gcggccagat atgtgtgatg gcaaagattc
    20281 gccatcgcgc atgtcgctta agccgcgctt atgggtagaa tcccggaaaa aatccgaatc
    20341 acacgcggcg ttg
//
LOCUS       DAOPYX010000015        20332 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_2724_length_20332_cov_14.584455, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000015 DAOPYX010000000
VERSION     DAOPYX010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 20332)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20332)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20332
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2724_length_20332_cov_14.584455"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..478)
                     /locus_tag="PKN55_01635"
     CDS             complement(<1..478)
                     /locus_tag="PKN55_01635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX69976.1"
                     /translation="MTTPSSPSSRPQPISLDVDIELTELTPAEGLPTRSPANPASVDM
                     QALLAREEAAFARIFRKGKPARARSAAPAAPPLKPAPVSEPSAADLPPADTVTAAPVL
                     PASEELDAFVGELLASAEHDRQIDAPPVLAQELAPADEAAPTLPASEELDAFVGELL"
     gene            complement(850..1494)
                     /gene="yihA"
                     /locus_tag="PKN55_01640"
     CDS             complement(850..1494)
                     /gene="yihA"
                     /locus_tag="PKN55_01640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HNX69977.1"
                     /translation="MPLFQNAAFFTTVNHLRDLPDTRAEVAFVGRSNAGKSSAINTLA
                     NRTRLAYVSKTPGRTQHINFFDLGHERFLVDLPGYGYAEVPEAVRAHWVELLGRYLGE
                     RSSLIGLLLIMDARHPLKDLDRRMLDYFTPTGRPVHILLSKADKLSRQEQATTLRKVK
                     AELAQYGDTVSVQLFSSLKKQGMDTVEQVVQTWFDAVDAAEVGAVDADDTAADE"
     gene            1798..2421
                     /locus_tag="PKN55_01645"
     CDS             1798..2421
                     /locus_tag="PKN55_01645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014085589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HNX69978.1"
                     /translation="MKVTKLLAVAGLATAGFAFANSPAAPTAKADPAKGKVLAEQVCA
                     ACHGADGNSVAAANPSLAGQHADYIVKQLTEFKSGARKNPIMMGMAAPLSPEDMKNVG
                     AYFAEQKIKPREAADKKLLPEGQKIFKGGVASTKIPACMACHGPNGAGIPAQYPRLGG
                     QHAGYIVAQMTAFRSGERANNTVMRDIALKMTDAQIKAVAEYISGLR"
     gene            2731..4695
                     /locus_tag="PKN55_01650"
     CDS             2731..4695
                     /locus_tag="PKN55_01650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011137933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein ResB"
                     /protein_id="HNX69979.1"
                     /translation="MRFAISLLTLLAIASVIGTVLKQNEPYPNYVVEFGQFWFTGFEW
                     LGLFDVYHSSWFLLILAFLVLSTSLCIWRNAPGFIKDMKGYREKASESSLAAMSHSVA
                     LPAGVSAAQASAYLRGQGFLVREASREDGVLVAAKKGAANKLGYFLAHIALVVICLGG
                     LLDGNVPLKVAELFGKVVPETRDIPQSQIPPQSRLSTSNLSFRGNVTIPENSSADVVF
                     LNAGNGYFVQDLPFLVTLKKFHVDYYSTGMPKLFASDLVVTDKQTGKETVATVKVNHP
                     LIIDGVAIYQASFGDGGSPLRFKAWNLATPAAPAQTLAAKSLGSQPVAINGQRYQLEA
                     GELRTFNIENVGDNNAAMGVNKTFQEVMQNAGQVKHDKQLRNLGPSITYKLRDAQGQA
                     REYHLYMSPISQDGRPYMMAGMRSEVAQPFAYLRLPLDDNNSLDDFMRLRAALMDAKV
                     QSEVARRVAAQALAGQAIGREMREPFEQSVRWVLDRFGQGGFAALESFLDERVPKDKR
                     KDVAQTYIKIVQGAVVEVDAVARERAGLPRRPVNEANYRFLIDSLVGVSALFDYGAPV
                     FMQLDSFDEVKATGLQITRSPGKTIVYLGSVLLVLGIICMFYIRELRVWVLVKSDQVR
                     VAMASNRKTSDLDRDFERHSNALQQLARGE"
     gene            4695..5840
                     /gene="ccsB"
                     /locus_tag="PKN55_01655"
     CDS             4695..5840
                     /gene="ccsB"
                     /locus_tag="PKN55_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019923450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcsB"
                     /protein_id="HNX69980.1"
                     /translation="MELVQPLHAPGFSLRQLNGRDWLYALVVLMAAGVAYSRYGHAMD
                     GYEQGILAGSALGLIALGWFWKSWQWYFPLSGALALGGLALYGDDLARGQTSFGLKFL
                     LSSQSAIMWMCFLYFLATAVYWLGLLRRSDFLNRVGSGLTWAATVLGLTGLFVRWGES
                     YLIGADVGHIPVSNLYEVFVLFCLITALMYLYYEARYQARQMGAFVLVVISAAVGFIL
                     WYTFDRQAHEIQPLIPALQSWWMKIHVPANFVGYGAFALSAMLGVAELLAMRGILTSK
                     LPKPEVIDEVMYKAIAVGFLFFTIATVLGAMWAADAWGGYWSWDPKETWALIVWLNYA
                     AWLHVRLVKGWRGAVLAWWAVIGLFVTSFAFIGVNMFLTGLHSYGTL"
     gene            6250..6945
                     /locus_tag="PKN55_01660"
     CDS             6250..6945
                     /locus_tag="PKN55_01660"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNX69981.1"
                     /translation="MTDPRPIRRNPDVLTDTLAVAGKQLIDVGCGNGHLVRHLARHGA
                     QVLGVECSPRQLAAAHAAARVADETIVEGVGQALPAADASADIVVFFNSLHHIPGEHM
                     ATALNEACRVLRADGVVYVAEPLAEGEFFALCRAVDDETQVRAAAQHAVLTHPRLRLA
                     QTFDYLHPVVLADFAAFRERLVSANHEREARFAAHQAELSALFARSGQPVDDGRQFIQ
                     PMRVHLLHPLPPG"
     gene            7160..8356
                     /locus_tag="PKN55_01665"
     CDS             7160..8356
                     /locus_tag="PKN55_01665"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008953693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid aminotransferase"
                     /protein_id="HNX69982.1"
                     /translation="MFEHVDAYAGDPILTLVETFNKDTRESKVNLGIGLYYDEAGRIP
                     LLESVRQAEAARAAQPAARPYQPMEGAANYRQAVQHLLFGAEHPAVKEGRIATIQTIG
                     GSGAIKIGADLLKRYFPASEVWVSSPTWDNHRSMFEGAGFKVHDYPYYDASTGGVNFA
                     AMLDTLKSLPAKSIVLLHPCCHNPTGVDLNQEQWLQVIAVAKANQLIPFMDIAYQGFG
                     DGLDEDAFAIRAMTEAGVSFLLSNSFSKNLSLYGERCGGLSVICQNAEEANRVLGQMK
                     FTVRRNYSSPPTHGGQVTAAVMNTAELRAIWEGEVAEMRVRIKAMRQKLFEVLTAKVP
                     GRDFSYFVKQRGMFSYTGLTPEQVDRLREEFAVYLVRSGRMCVAGLNTRNVEYVADAM
                     AAVLKG"
     gene            complement(8451..8843)
                     /locus_tag="PKN55_01670"
     CDS             complement(8451..8843)
                     /locus_tag="PKN55_01670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX69983.1"
                     /translation="MKIAIATDNYTRVAGHAGKARHWLLYLLHGHTPQQLLPAPTLIK
                     LAPEQVMHHFKDDAPHPLDGVDLVVAGSAGEGFIRHMRERGADVLLTGEDDPATALTQ
                     ILAGEALPDPRFDVTTTLCKLRDLFSRH"
     assembly_gap    8862..8961
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<8962..9808)
                     /gene="modD"
                     /locus_tag="PKN55_01675"
     CDS             complement(<8962..9808)
                     /gene="modD"
                     /locus_tag="PKN55_01675"
                     /inference="COORDINATES: protein motif:HMM:TIGR01334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ModD protein"
                     /protein_id="HNX69984.1"
                     /translation="MNPYCLPDSLLEQLLHEDSPYGDATSFALGLGAHQGELHFAARQ
                     AMTLCASEDAQRMAQMLGLHIDQPARASGARLAAGEPILTVSGAADALHRLWKPAQTL
                     MEYLSGIASAVSALVDAARAVSPDIGVACTRKTFPGGKAACIKAVLAGGAVPHRLSVS
                     ETLLLFAEHRTFLPDASPEAMLMALRSWSERKTVVEVADPAEARRWIDAGAEVIQLEK
                     CPPAHVAEVVAYARQRAAPPLIAAAGGVHAGNAADYARAGAGLLVSSAPYHAAPRDVA
                     VSMRAR"
     gene            complement(9884..10636)
                     /gene="modA"
                     /locus_tag="PKN55_01680"
     CDS             complement(9884..10636)
                     /gene="modA"
                     /locus_tag="PKN55_01680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011137273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HNX69985.1"
                     /translation="MWRPVLTSAAVLLAFAPLAQADEVAVAVAANFTAPMQKIAAEFE
                     KDTGHKAVLSFGATGKFYAQITNGAPFEVLLSADDETPAKLEKEQAGVAGSRFTYAIG
                     KLVLWSAKPGYVDSKGEVLKKGDFARLSIANPKTAPYGQAAMETLDKLKVLPALQPKI
                     VQGENISQAHQFVATGNAELGFVALSQVYVDGKLTSGSMWQVPGTLYSPIRQDAVVLN
                     KGAGKPAVQALMTYLKSAKARAVIHSYGYDLP"
     gene            complement(10770..>11592)
                     /locus_tag="PKN55_01685"
     CDS             complement(10770..>11592)
                     /locus_tag="PKN55_01685"
                     /inference="COORDINATES: protein motif:HMM:TIGR00637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HNX69986.1"
                     /translation="VMHPSLPRLRGKLEVDTELGTFLGDTRIRLLEAIAKHGSISQAA
                     KAVPLSYKAAWDAIDAMNNLAEHPLVTRVTGGRHGGGSTLTPYGEKVVALYRALESEY
                     QAALDRLAANMHSSEHGDFAQFRQLLKRMSMRSSARNQFAGTVCALREGDVDYEVRLR
                     LDADNELVAVITRESAENFQLHMGQELYALVKSSSILLLTDNKVKTTARNQLWGEISR
                     IVDGPVNAEVTLVMPGGKSLCAVVTHDSLERLGLEVGGKVCAVFKASAVILCSYQ"
     assembly_gap    11593..11692
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<11693..11995)
                     /locus_tag="PKN55_01690"
     CDS             complement(<11693..11995)
                     /locus_tag="PKN55_01690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX69987.1"
                     /translation="MRASAAAVEGAHNWRGYLPDAWADRVEAPLWVEEFQEYQLDAQR
                     WVGYDADEQPCYTAHHYALPAQDASAGAAVAYSEMLAAWRLRDERWLVFRLIGAGGA"
     gene            complement(12406..13788)
                     /gene="argH"
                     /locus_tag="PKN55_01695"
     CDS             complement(12406..13788)
                     /gene="argH"
                     /locus_tag="PKN55_01695"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017507823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HNX69988.1"
                     /translation="MQDAHAWSGRFSEPVSELVKKYTASVDFDKRMAAFDIQGSLAHA
                     AMLTRAGVLSEADLSAIEAGMAEILQDIQEGRFEWSVDLEDVHMNVEKRLTDKIGDAG
                     KRLHTGRSRNDQVATDIRLYLRDAIDHIVTLIAALQTSLLDLAEQHADTVMPGFTHLQ
                     VAQPVTFGHHMLAYVEMLGRDGERMVDCRKRVNRLPLGAAALAGTTFPVDRFYTAQLL
                     GFDDVCHNSLDAVSDRDFAIEFTAAASLVMVHLSRLSEELILWMSPRVGFIDIADRFC
                     TGSSIMPQKKNPDVPELVRGKSGRVTGHLLALLMLMKSQPLAYNKDNQEDKEPLFDTV
                     DTLIDTLRIYADMMRGVTVKPEAMRAAVLQGFATATDLADYLVKKGVPFRDSHEVVAR
                     AVRHAEVQGVDIADLSLDVLRQFSALIDDDVYAVLTPEGSLAQRNHIGGTAPVQVRAQ
                     IARHRARLQG"
     gene            complement(13877..14215)
                     /locus_tag="PKN55_01700"
     CDS             complement(13877..14215)
                     /locus_tag="PKN55_01700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX69989.1"
                     /translation="MLLLTRSLALAGALLAAVAAAQTIDDDLLAAQMNYQRATRLAEK
                     ARQEADLARQNRQNAEGQLVTAQRALDAAQAEQARAEAAERDAVTDLGLARQRLDATW
                     GVKQQRSAQP"
     gene            14318..17797
                     /gene="recC"
                     /locus_tag="PKN55_01705"
     CDS             14318..17797
                     /gene="recC"
                     /locus_tag="PKN55_01705"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HNX69990.1"
                     /translation="MAAGELDPGLMIWHGNQLEDLRDVLAAWLRRQPLAPLENEIILV
                     QSQGMGQWLKLALAGADGVSAAVSMQLPSQFLWRAYRDVLGPDAVPADSPLERRTLVW
                     RLMRLLPALSAGPEFAVLRGFLAGDDDGRKLFQLAGKLAELFDQYQVYRADWLADWQA
                     GRDGLAQTDGQWRALTDDDRWQPALWRALLADIAADGHQDIGRAQIHQQFLQALAAAP
                     SRPPALPRRVVVFGISALPQQALAALAALGRHCQVILAVHNPCQHYWGDLDDPKTIAR
                     RLARRGAGALPSRDSLYADNQPLLAAWGRHGRDFLALLDQYDQPERYQPQLASQGQRI
                     DLFTEPDASRLLGQLQHGIFDLTPPPASPEARQPVAADDDSLCFHLAHSRQREVEILH
                     DQLLAYCEAAQAAGAPLAPRDIVVMTPDVAAYAPHVRAVFGRIPPGDPRHLPFTLADR
                     RARDSSPLLRALDALLASPHARFTVSEVFDLLDAPALARRFGLQASQLPALRQWAEQA
                     GVRWGLDADQRAQLNLPDGLNQNSWAFGLERLLLGYAVGDGDAWDGRIQPCDEAAGLE
                     VAALGPLAQLVDALRRWSRVLAEPAAPAQWGERLRQLMADFLLPDDDHERRMLLRLDS
                     ALEDWLSACADAGLHAALPLAVAREGWLAGVDEAAPSQQFLAGAVNICTLMPMRAIPF
                     RVVCLLGMNDGDYPRRQPPSDFDLMARPYGQRPGDRARRDDDRYLLLEALLSARERLH
                     ISWVGRSVRDNSPLPPSMLIGQLRDALAAGWRLADDPEASGERLLAALSCEHPLQPFS
                     PRYFSGEDPRLFTYASEWRAAHDRADAAPTALPPATPAVTLVGVTELARLLRRPTAHF
                     FQRRLRVNFANADDSLLEHEPFELSGLDGYALRERLFAALRRGARDGLSAEAVCAAEL
                     ARWQREGVLPLQAAEARFAALAEQVTPVWQAWQAQAAHCPARPAREISLSLAHGLQLE
                     DWVRELHDSEAGLLRLLALPHPLRHSGGWRAERLLAPWLAHLAVNADHTPLRTCLLAS
                     DDGEHGPLCWPALPAEQAQSLLASVAEAWRAGLDAPLPLACKTTLAYLQYRLRHADDE
                     PEEVEAAAWQAASQTFIGGWNLRGEVDDDPYLARAYPDWPALLAGGERARDWAERLYL
                     PLLHATLSPKECL"
     gene            17797..>20332
                     /locus_tag="PKN55_01710"
     CDS             17797..>20332
                     /locus_tag="PKN55_01710"
                     /inference="COORDINATES: protein motif:HMM:NF024642.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HNX69991.1"
                     /translation="MSQPLNPLTLPLSGSWLIEASAGTGKTYTLAALYVRLALGHGPD
                     DGARLPALSPSQILVMTFTEAAAGELRDRIRQRLTGLADWLRRDAPAAEADAFSLGLL
                     GELDEADGLGREQAARRLALVADLLDDAAISTIHSWCLRMLREHALYSGNLFDESLDS
                     DDGARYADSVRDYWRTFVYPLSDADFAAVAVHWATPDQLADALRPLLGELPEPPAVWR
                     GPLALARGRQHSLQEAAALKTGWPERVAELRARFADWQAAKAYNGNRLRAATLAKLLD
                     ALEAWASQPHLAQLDDSLDWSRLSPDFLADIWKGDGPPPCEPLSALATLHTQLAALTP
                     FHHGVLAHASQWLGHVLDAHKRREAVMDFDDLLIRLDQALQTDASHPDPEQRGRLAAA
                     IRQQFPAALVDEFQDTDPRQYRILDALYQVAAPHPGTLLAMIGDPKQAIYAFRGGDIF
                     TYLTARAAAHGRHVTLDTNFRSTEAMVARVNAMFARAEARPGGAFAFADDAGAPLLPF
                     QPVRAAGRRETLHHQGAPLAALQVWLDAEPLNAASYRERMAERCAAQVCALLAGAQAG
                     DTGFADADGGWRALTAADIAILVRDGGEAKSVRAALTRRGIQSVYLSDRDSVFASREA
                     ADLLHWLRACAEPTQERWVRAALATATLDLPYARLDAQSEAGARDWDAEVERFVALNA
                     CWRRQGVLPMLRQLLHRFDVPARCLARDGGERALTNLLHLAELLHADAELLDGEHALI
                     RRLAERIARPDRGGDERIVRLESDAGLVRVVTIHKSKGLEYPLAMLPFAASYRPVDGA
                     RLPAYRYHQKDGDAYRPALSLFKREDERLAQDRERLQEDLRLFYVAV"
BASE COUNT         3322 a         6638 c         6660 g         3512 t
ORIGIN      
        1 ccagcagttc gccaacaaaa gcgtccagtt cttcgctggc cggcagggta ggcgctgctt
       61 catcagcagg agccagttct tgcgccagca ccggcggtgc gtcgatctgg cgatcgtgct
      121 cggcactggc cagcaactca ccgacaaaag cgtccagttc ttcactggcg ggcagaacgg
      181 gcgcggccgt cacggtgtcg gcgggcggca aatcagcggc agaaggctcg ctcactggcg
      241 cgggcttcag tggcggcgca gcaggtgccg cactgcgcgc acgcgccggc ttgcccttgc
      301 ggaagatgcg cgcaaaggcc gcttcttctc gcgccagcag ggcctgcatg tccacgctgg
      361 ccggattggc aggcgagcgg gtgggcaagc cttcggcggg agtgagttct gtgagttcaa
      421 tatccacatc cagcgagatc ggttgcggac gggaggaagg cgaggaagga gtcgtcatga
      481 caggcgctgc gcttcaggtg acggggcaaa aagccgatgc gccatgatac tgaattgcgc
      541 ctgcttacgg tagcggcaat gcagggcgca tgcggcaatt atattgcaac gcatcatggc
      601 gccccggcac tgcgcaagag ttccattcgg atcagggggt gcttcatgcg acacaccacc
      661 cggcaaacgg cttggtaaat ccatctggcg cagcggagcc gccttgccta cccgcgccag
      721 gtggatgggc gctttttaga gccgctagca aaactcagcg aagcggccct ggctaggcgt
      781 gtgaccgcag acagtacaac aagtacggca aggtcacaca acaacgccag ggggttttgt
      841 gagcggctct tattcgtcgg cggcggtatc gtcggcatcc actgcaccaa cctcggcggc
      901 gtccaccgcg tcaaaccagg tttgcaccac ttgctcgacg gtgtccatgc cctgtttttt
      961 caggctggag aacaattgca cgctgacggt gtcgccgtat tgggccagtt cggccttgac
     1021 cttgcgcagg gtggtggctt gttcctggcg gctgagtttg tcggctttgg acaataaaat
     1081 atgcaccggc cggccggtgg gggtgaagta gtccagcatg cgccggtcga ggtctttcag
     1141 cggatggcgg gcgtccatga tcagcaagag gccaatcagc gagctgcgct cgcccagata
     1201 gcggcccagg agctccaccc agtgggcgcg cacggcttcc ggcacttcgg cgtagccata
     1261 accgggcaag tccaccagaa agcgttcgtg gcccaggtca aaaaaattga tgtgctgggt
     1321 gcggccgggg gttttcgaga cataagccag ccgggtgcgg ttggccaggg tgttgatggc
     1381 gctggatttg ccagcgttgg agcggccgac aaaggccact tcggcgcggg tgtccggcag
     1441 gtcgcgcaag tgattgacgg tagtgaaaaa agcggcgttt tgaaacagcg gcatggcatt
     1501 ctttcaggcc ggcgcggcgc gccggatggc aaaggcgata ttgtctgcgc gcagggtcgg
     1561 caaggccagc gctttttttg cccgcccggc ccatgccatc aagctaattg gccgtggcca
     1621 ctgcgtattt tcaccaaaac cctgctcggg ccagtggtaa cggcaaatcc ctttggtata
     1681 gaatagtaag gttcaaaatt ctactttccg acagcggtca agcttgctgt tcgcgcccga
     1741 ttgtgcggtc ctcgggccgg gcgggcgccg ctgggccaaa aatctcaagg agttctcatg
     1801 aaggttacca agctgctggc tgtggccggc ctcgcgacgg cagggtttgc ttttgccaac
     1861 tcgccggctg ctccgaccgc caaagcggat ccggccaagg gcaaagtgct ggccgagcaa
     1921 gtgtgcgcgg cctgtcacgg cgcggacggc aacagcgttg ccgccgccaa ccccagcctg
     1981 gccggccagc atgccgacta catcgtcaag cagctgaccg agttcaagtc gggcgcgcgc
     2041 aaaaacccga tcatgatggg catggccgct ccgctgtcgc cggaagacat gaaaaacgtc
     2101 ggcgcatact ttgccgagca gaagatcaag ccgcgcgaag ccgccgacaa gaaactgctg
     2161 ccggaaggcc agaagatctt caagggcggc gtggccagca ccaagatccc ggcttgcatg
     2221 gcctgccacg gcccgaacgg cgccggcatt ccggcccagt acccgcgtct gggcggccag
     2281 cacgctggct acatcgtggc gcagatgacc gccttccgca gcggcgagcg cgccaacaac
     2341 acggtgatgc gcgacatcgc cctgaagatg accgacgccc agatcaaggc cgtggccgag
     2401 tatatctccg gcctgcgctg atcccggacg caactttttc gacggcgttt cgtccaagcc
     2461 gcgctggttt attcgccagc ggcggcgctg tcctgccaac ggcccgcgcg cattgtctgc
     2521 tcgcgggccg ttggccgtca tggccgcccg cgccgcgcca tgcgatactg ccgcccggac
     2581 gccggctgac ggcgatttga tagatttttc ctccgcaata tgcggcgctt gcgccggccg
     2641 tggcggagtc tgattgtgac gtgaatgcct atgcgcgcgc cttcccgctc gagccctgcg
     2701 ctcgccagcc tctacgagct gttcagctcc atgcgttttg ccatcagcct gctgaccttg
     2761 ctggcgattg cctcggtgat tggcaccgtg ctcaagcaaa acgaacccta ccccaattat
     2821 gtggtggaat tcggccagtt ctggtttacc ggctttgaat ggctgggcct gttcgatgtg
     2881 taccattctt cgtggttttt gctgattctg gcttttctgg tgctgtccac cagcttgtgc
     2941 atctggcgca atgcgccggg ctttatcaag gacatgaagg gctaccgcga gaaagccagc
     3001 gaaagctcgc tggcggccat gtcgcacagc gtggcgctgc cggcgggcgt cagcgccgcc
     3061 caggccagcg cctatctgcg cggccagggg tttttggtgc gcgaggccag ccgcgaagac
     3121 ggcgtgctgg tggcggccaa aaagggcgcg gccaacaagc tggggtattt tctggcgcac
     3181 atcgccctgg tggtgatttg cctgggcggc ctgctggatg gcaatgtgcc gctgaaagtg
     3241 gcggagctgt ttggcaaggt cgtgccagaa acccgggata ttccgcaaag ccagattccg
     3301 ccgcaaagcc ggctgtccac cagcaatctg tcgtttcgcg gcaatgtcac catcccggaa
     3361 aacagcagcg ccgatgtggt gtttctgaat gccggcaatg gttattttgt gcaggatttg
     3421 cccttcctgg tgacgctgaa aaaattccat gtcgattatt acagcaccgg catgcccaag
     3481 ctgtttgcca gcgatctggt ggtgaccgac aagcaaaccg gcaaggaaac cgtggccacg
     3541 gtcaaggtga accacccgct gatcatcgac ggcgtggcga tttatcaggc cagctttggc
     3601 gacggcggct cgccgctgcg ctttaaggcc tggaacctgg ccacacccgc agcgccggcg
     3661 caaaccctgg cggccaaatc gctgggcagc cagccggtgg caatcaacgg tcagcgctac
     3721 cagctggaag ccggcgagct gcgcaccttc aatatcgaga acgtcggcga caataacgcc
     3781 gccatgggcg tgaacaaaac cttccaggag gtgatgcaga acgccggcca ggtcaagcac
     3841 gacaagcagc tgcgcaatct ggggccgtcg atcacctaca agctgcgcga tgcccagggc
     3901 caggcgcgcg aatatcattt gtacatgtcg ccaatcagcc aggatggccg gccgtacatg
     3961 atggccggca tgcgcagcga agtggcgcag ccgtttgcct atctgcgcct gccgctggac
     4021 gacaacaaca gcctggacga cttcatgcgc ctgcgcgcgg cgctgatgga cgccaaggtg
     4081 caaagcgaag tcgctcgccg ggtggcggcg caagcgctgg ccggccaggc gattggccgc
     4141 gaaatgcgcg agccgtttga gcaaagcgtg cgctgggtgc tggaccgctt tggccagggc
     4201 ggctttgccg cgctggaaag ctttttggat gagcgggtgc ccaaggacaa gcgcaaggac
     4261 gtggcgcaga cctatatcaa gatcgtgcaa ggcgccgtgg tggaggtgga cgccgtggcg
     4321 cgcgagcgcg ccggcctgcc gcgccggccg gtcaacgaag ccaattatcg cttcctgatc
     4381 gacagcctgg tgggggtcag cgccctgttt gactacggcg cgccggtgtt catgcagctg
     4441 gacagttttg acgaagtcaa ggccaccggc ctgcaaatca cccgttcgcc gggtaagact
     4501 atcgtctatc ttggctcggt gctgctggtg ctgggtatca tctgcatgtt ttacatccgc
     4561 gaactgcgcg tgtgggtgct ggtcaagtct gatcaggtcc gcgtggcgat ggcgtccaac
     4621 cgcaagacca gcgacctgga tcgggatttc gagcgccaca gcaatgcgct gcaacaactg
     4681 gcaagaggtg aatgatggaa ctggttcaac cgctgcacgc gccggggttt tccctgcgcc
     4741 agcttaatgg ccgcgactgg ctgtacgccc tggtggtgct gatggcggcc ggcgtggcgt
     4801 attcccgcta tggccacgcc atggatggct acgagcaagg cattctggcc ggctcggcgc
     4861 tgggcctgat tgcgctgggc tggttctgga aaagctggca atggtatttc ccgctgtccg
     4921 gcgcgctggc gctgggcggg ctggccttgt atggcgatga tctggcgcgc gggcaaacca
     4981 gctttgggct gaagtttctg ctcagcagcc aatcggccat catgtggatg tgttttctgt
     5041 attttctggc cacggcggtg tactggctgg gcctgctgcg ccgctcggat tttctcaacc
     5101 gcgtcggcag cgggctgacc tgggccgcca ccgtgctggg cctgaccggc ctgtttgtgc
     5161 gctggggcga gagctatctg attggcgcag acgtcggcca tatcccggtg tccaatctgt
     5221 acgaagtgtt tgtgctgttc tgtctgatca ccgcgctgat gtatctgtat tacgaagcgc
     5281 gctatcaggc ccggcagatg ggcgcctttg tgctggtggt gatttccgcc gccgtgggtt
     5341 ttatcctgtg gtacaccttc gaccgtcagg cgcatgaaat ccagccgctg attccggcgc
     5401 tgcaatcgtg gtggatgaaa atccacgtgc cggccaactt tgtcggctat ggcgcctttg
     5461 ccctgtcggc catgctgggc gtggcggagc tgctggccat gcgcggcatc ctcaccagca
     5521 agctgcccaa gccggaagtg atcgacgagg tgatgtacaa ggccatcgcc gtgggctttt
     5581 tgttcttcac catcgccacc gtgctgggcg ccatgtgggc ggccgacgcc tggggcggct
     5641 actggagctg ggatccgaaa gaaacctggg cgctgattgt ctggctgaat tacgccgcct
     5701 ggctgcatgt gcgtctggtc aagggctggc gcggcgcggt gctggcctgg tgggcggtga
     5761 tcgggctgtt tgtcaccagc tttgccttta tcggcgtgaa tatgttcctg accggtttgc
     5821 actcttacgg cacgctgtaa aaaaattctt gtattcctga ccgaaaccgt ctttactgac
     5881 ttcagggcgg cagcaatcgc gccgccctga caaaccggtt gccatccagc cggtttgccc
     5941 gaagttgccg gtggtctgat cggcggaaag acctggagcg atgctcctca ccgcctgcac
     6001 gcgttttccc ggcgcgtaga cccttcaccg gccggagctg cgtctctgtg cagcatgtcg
     6061 ccaacccagc gcctgcgcct tgtgcgtgcg gcgagtcttc aacctgcatc atttcattga
     6121 agtcacgagt tgtttgtacg ttacgctccg tcacgaggcc gcctttgggc ggcctctttc
     6181 catttgtagc ccgcgcggca aacactctat tacaatgtga actatctgct cacttcagag
     6241 gcgcccacca tgaccgaccc ccgcccgatt cgccgcaacc cggacgtgct caccgacacc
     6301 ctggcggtgg ccggcaaaca gctgatcgac gtcggctgcg gcaatggcca tctggtgcgt
     6361 cacctggccc gccacggcgc gcaggtgctg ggggtggaat gcagcccgcg ccagctggcc
     6421 gccgcgcacg cagccgcacg ggtggccgac gaaaccatcg tcgaaggcgt gggccaggcc
     6481 ttgcccgctg ccgacgccag cgccgacatc gtggtgtttt tcaactcgct gcaccatatc
     6541 cctggcgagc acatggccac cgcgctcaac gaagcctgcc gcgtgctgcg cgccgacggc
     6601 gtggtgtacg tggccgaacc gctggctgaa ggtgagtttt ttgccctgtg ccgcgccgtg
     6661 gacgacgaaa cccaagtgcg cgccgccgcc cagcacgccg tgctcaccca cccgcgcctg
     6721 cgcctggcgc agacgtttga ctatctccac ccggtggtgc tggccgactt tgccgccttt
     6781 cgcgagcggc tggtgtcggc caatcacgag cgcgaagcgc gctttgccgc gcatcaggcc
     6841 gagctcagcg ccctgtttgc ccgctcgggc cagccggtgg acgatggccg gcagtttatc
     6901 cagccgatgc gcgtgcatct gttgcatccg ctcccgccgg gctgagcgcc gcacagcggt
     6961 gtgggcgcca gaaatgtcgc acgatagcca cgccaacggc gcataagcaa ttgcccgccg
     7021 ccccggattg ggggatgatt ggcgttcatt gtcgattcac tgcactgtca tggtgcggca
     7081 atgcgttcca cccctgctta tgccctggcg gcgtcgcccc tgaccgctgc ccacaacctc
     7141 tacaggatgc tgttttgcca tgtttgaaca tgtagacgcc tacgccggcg acccgattct
     7201 gaccctggtc gaaaccttca acaaagatac ccgcgaatcc aaggtgaacc tgggcatcgg
     7261 cctctactac gacgaagccg gccgcattcc gctgctggaa tcggtgcgcc aggccgaagc
     7321 cgcccgcgcc gcccagccgg ccgcccgccc gtaccagccg atggaaggcg ccgccaacta
     7381 tcgccaggcc gtgcaacacc tgctgtttgg cgccgagcac ccggccgtca aagaaggccg
     7441 catcgccacc atccagacca tcggcggctc tggcgccatc aagatcggcg ccgacctgct
     7501 caagcgctac ttcccggcca gcgaagtgtg ggtcagcagc ccgacctggg acaaccatcg
     7561 ctcgatgttc gaaggcgctg gcttcaaggt gcacgactac ccgtactacg acgccagcac
     7621 cggcggcgtg aactttgccg ccatgctcga caccctgaag tcgctgccgg ccaagagcat
     7681 cgtcctgctg cacccgtgct gccacaaccc caccggcgtg gacctgaacc aagagcaatg
     7741 gctgcaagtc atcgccgtgg ccaaggccaa ccagctgatc ccgttcatgg acatcgccta
     7801 ccaaggcttt ggcgacggcc tggacgaaga cgccttcgcc attcgcgcca tgaccgaagc
     7861 cggcgtgagc ttcctgctga gcaactcgtt ctccaagaac ctgtcgctgt acggcgagcg
     7921 ctgtggcggc ctgtcggtga tctgccagaa cgccgaagaa gccaaccgcg tgctgggcca
     7981 gatgaagttc accgtgcgcc gcaactactc cagcccgccg acccacggcg gccaggtgac
     8041 cgcagcggtg atgaacaccg cagaactgcg cgccatctgg gaaggcgaag tcgccgaaat
     8101 gcgcgtgcgc atcaaggcca tgcgccagaa gctgtttgaa gtgctgaccg ccaaggtgcc
     8161 gggccgcgac ttcagctact tcgtcaagca acgcggcatg ttcagctaca ccggcctgac
     8221 cccggaacaa gtggaccgcc tgcgcgaaga gttcgccgtt tacctggtgc gctccggccg
     8281 catgtgcgtg gccggcctga acacccgcaa cgtcgaatac gtggccgacg ccatggccgc
     8341 cgtgctgaag ggctgattca gccccaagcc aatctggcgc tgcgctgagt ttgcgcgagc
     8401 atgacaaaac ccccgctggt gacagcgggg gtttttgttg gcgcacacgc tcaatgccgc
     8461 gaaaacagat cgcgcaattt acacagcgtg gtggtgacgt caaaacgcgg gtcgggcagc
     8521 gcttcgccgg ccaggatttg cgtcagcgcc gtggccgggt cgtcttcgcc ggtcagcagc
     8581 acatcggcgc cgcgctcgcg catgtggcgg ataaagccct cgccagcgct gccggccacc
     8641 accagatcca ccccgtccag cgggtgcggg gcatcgtcct tgaagtgatg catcacctgc
     8701 tcgggggcca gcttgatcag cgtgggcgcc ggcagcagtt gttgcggggt gtggccgtgc
     8761 agcaggtaaa gcagccagtg gcgcgccttg ccggcgtggc cggcgacgcg ggtgtaatta
     8821 tcggtggcaa tggcgatttt catggtcggt cacagtgaaa annnnnnnnn nnnnnnnnnn
     8881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8941 nnnnnnnnnn nnnnnnnnnn naacgcgccc gcatgctcac cgccacatcg cgcggcgcgg
     9001 cgtggtaggg cgcgctgctg accagcaggc cggccccggc gcgggcgtag tcggcggcgt
     9061 tgccggcgtg cacgccgcct gcggcggcaa tcagcggcgg cgcggcgcgc tggcgggcgt
     9121 aagccaccac ctcggccacg tgggccgggg ggcatttttc cagttgaatc acctcggcgc
     9181 cggcatcgat ccagcggcgc gcttcggccg ggtcggccac ttccaccacg gtcttgcgct
     9241 cgctccagct gcgcagcgcc atcagcatgg cctcggggct ggcgtcgggc aaaaatgtgc
     9301 gatgttcagc aaacagcagt agggtttccg acacactcag ccggtgcggc acggcgccgc
     9361 cggccaacac cgccttgatg caggcagcct tgccaccagg aaacgtcttg cgtgtgcagg
     9421 ccacgccaat atccgggctg accgcgcggg cggcgtccac cagcgcgctg accgcgctgg
     9481 caatccctga tagatactcc atcagcgttt gcgccggctt ccacagccga tgcagcgcat
     9541 cggccgcgcc gctcaccgtc aggataggct cgccggcagc cagtcgggcg ccgctggcgc
     9601 gggcaggctg atcaatgtgc aggccgagca tctgcgccat gcgctgggcg tcttcgctgg
     9661 cgcacagcgt catcgcctgg cgggcggcaa aatgcagctc gccctgatgc gcgcccaggc
     9721 ccagggcaaa gctggtggcg tcgccgtatg ggctgtcttc gtgcaataac tgttccagca
     9781 gggaatcggg caggcagtag gggttcatgg cgggctcatc gcagcagcaa ggggacacca
     9841 gacacgagca aggcgcgcgc cagcgtccgc ctgccgccat cggtcagggc aaatcgtagc
     9901 cgtaggaatg aatcaccgcg cgcgccttgg cgctcttcag gtaggtcatc aacgcttgca
     9961 ccgccggctt gccggcgccc ttgttcaaca ccacggcgtc ctggcgaatc gggctgtaca
    10021 gcgtgcccgg cacttgccac atcgagccgg aagtcagctt gccgtccaca tacacttgcg
    10081 acagggcgac aaagcccaat tcggcattgc cggtggcgac aaactgatgc gcctgcgaga
    10141 tattttcccc ctgcacgatc ttgggctgga gcgcgggcag cactttcagc ttgtccaggg
    10201 tttccattgc cgcctggcca taaggggcgg ttttcgggtt ggcgatcgac aggcgggcaa
    10261 agtcgccttt tttcagcacc tcgcccttgc tgtccacata gcccggcttg gccgaccaca
    10321 gcaccagctt gccaatggcg taggtaaagc ggctgcccgc cacgccggct tgttcttttt
    10381 ccagcttggc cggggtttcg tcgtcggccg acagcagcac ctcaaacggt gcgccattgg
    10441 tgatctgggc gtagaacttg ccggtggcgc caaacgacag caccgccttg tggccggtgt
    10501 ctttctcgaa ttcggcggcg attttttgca tcggcgcggt aaagttggcg gcaacggcga
    10561 cggcaacttc gtcagcttgg gccaaggggg caaaagccag cagcacggcg gcagaggtca
    10621 gaacgggacg ccacatggca aaactcctgg gtgggtgaaa aatgggttga ttgatcgcgc
    10681 agcaatcctg gctaggcgcg cggtcgcaga cagtacaagg agtacggcaa ggtcgtgcaa
    10741 caacgccagg agggttttgt gagcgactct tactgatagc tgcacaaaat cactgccgaa
    10801 gccttgaaca ccgcgcacac cttgccgcca acttccagcc ccagccgctc caggctgtcg
    10861 tgggtgacca cagcgcacag cgatttgccg ccgggcatca ccagggtgac ttcggcgttg
    10921 accgggccat cgacaatgcg gctgatttcg ccccacagct gattgcgcgc ggtggttttc
    10981 accttgttgt cggtcagcag caaaatcgac gaggacttca ccagcgcgta cagctcctgg
    11041 cccatatgca gctgaaagtt ctcggcggac tcgcgggtga tcaccgccac cagctcgtta
    11101 tcggcgtcca gacgcaggcg cacttcgtaa tcgacatcgc cctcgcgcag cgcacacacc
    11161 gtgccggcaa actggttgcg cgcgctgctg cgcatcgaca tgcgcttgag caactggcgg
    11221 aactgggcaa aatcaccgtg ctcgctgctg tgcatattgg cggccagacg gtccagcgcg
    11281 gcctgatatt cgctttccag cgcgcggtac agcgccacca ctttttcgcc atacggcgtc
    11341 agcgtcgagc cgccgccgtg ccgcccgcca gtcacgcggg taaccagcgg atgctcggcc
    11401 agattgttca tggcgtcgat ggcgtcccac gccgccttgt aagacaaggg caccgccttg
    11461 gccgcctgcg aaatcgagcc atgcttggca atggcttcca gcaggcggat gcgggtgtcg
    11521 cccaaaaacg tgcccagctc ggtgtccact tccagcttgc cgcgcaagcg tggtaaagaa
    11581 ggatgcataa cgnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnggcgccgc
    11701 cggcgccgat caaacgaaac accagccagc gctcgtcacg cagccgccag gcggccagca
    11761 tttctgaata cgccaccgcc gcgcctgcgc tggcgtcctg cgccggcagc gcgtaatggt
    11821 gggcggtgta gcacggctgc tcgtcggcgt cgtagcccac ccagcgctgg gcgtccagtt
    11881 gatattcctg gaactcctcc acccacaatg gcgcttccac ccgatcggcc caggcgtcgg
    11941 gcaaatagcc gcgccagttg tgcgcgccct ccacggcggc cgcgctcgcg cgcatggtgt
    12001 ctgcccagct ttgtcgccct tcctgcctgt ccagtctcat cgcgcgcctc ctgcttgctg
    12061 tttttgccat ccgcctggcc ggtggcctgt ctttcatgcc cagcagatgc catgccagtt
    12121 tatttattca tttaaatcag ctaattacac caacaccacc acgagcagct gttcgatgta
    12181 taacaaaata tacaacgatt gtcgcacaca tgccatacgc catccggcag acaagacaac
    12241 gccccgcacc ggcaaaccgg gcggggcgtg tcaaccaggg agaaccactc acagcactca
    12301 gcgaagcggc cctggccagg cgtgtgaccg caaaccgtac acgccgtatg gcaaggccgc
    12361 gaacaacccc aagggggttt tttgcggcgc ttagccgacc ggcaatcagc cttgcaggcg
    12421 ggcgcgatgg cgggcgattt gcgcgcgcac ttgcaccggc gcagtgccgc cgatgtggtt
    12481 gcgctgggcc agcgagcctt ccggggtcag cacggcgtac acgtcatcgt caatcagcgc
    12541 gctgaactgg cgcagcacat ccagcgacaa gtcggcgatg tccacaccct gcacttcggc
    12601 gtggcgcacg gcgcgggcca ccacttcgtg gctgtcgcgg aacggcacgc cctttttcac
    12661 caggtagtcg gccaggtcgg tggcggtggc aaagccttgc agcacggcgg cgcgcatggc
    12721 ttcgggcttg acggtgacgc cgcgcatcat gtcggcgtag atgcgcaggg tgtcgatcag
    12781 ggtgtccacc gtgtcgaaca gcggttcttt gtcttcctga ttgtctttgt tgtaggccag
    12841 cggctgcgat ttcatcagca tcagcagcgc cagcagatgg ccggtgacgc ggccgctctt
    12901 gccgcgcacc agctcgggca cgtccgggtt tttcttttgc ggcatgatcg agctgccggt
    12961 gcagaagcgg tcggcaatat caataaagcc cacgcgcggg ctcatccaca ggatcagctc
    13021 ttccgacagg cggctcaaat gcaccatcac cagcgaggcg gcggcggtga attcgatggc
    13081 aaagtcacgg tcggacaccg cgtccagcga gttgtggcag acgtcgtcaa accccagcag
    13141 ctgggcggtg taaaaacgat ccaccgggaa ggtggtgccg gccagcgcgg cggcgcccag
    13201 cggcaggcgg ttaacccgct tgcggcaatc caccatgcgc tcgccgtcgc ggccgagcat
    13261 ttccacatag gccagcatat ggtggccaaa ggtcaccggc tgcgccacct gcaagtgggt
    13321 gaagcccggc atcaccgtgt cggcgtgctg ttcggccaga tccagcaggc tggtttgcag
    13381 cgcggcaatc agggtaacga tatggtcgat ggcgtcgcgc agatacaggc ggatgtcggt
    13441 ggccacctgg tcgttgcggc tgcggccggt gtgcaggcgc ttgccggcgt cgccgatttt
    13501 gtcggtcagg cgcttttcca cgttcatgtg cacgtcttcc aggtccacgc tccattcaaa
    13561 gcggccttcc tggatgtctt gcagaatctc ggccatgccg gcttcgatgg cgctcagatc
    13621 ggcttcggac agcacgccgg cgcgggtgag catggcggcg tgggccagcg agccctggat
    13681 gtcgaacgcg gccatgcgtt tatcgaaatc aaccgaggcg gtgtattttt tgaccagttc
    13741 ggaaaccggc tcggaaaagc ggccggacca ggcgtgcgcg tcttgcatgg cggagtgtcc
    13801 tgaaaagatg ggcgtcgccg gcctgcggca ggcgacggaa ttcggggtaa aaacgcgatt
    13861 atatcagccg cgcggctcag ggctgggcgc tgcgctgctg cttgacgccc caggtggcgt
    13921 ccagccgctg gcgggccagg cccagatcgg tgaccgcgtc gcgctcggcc gcctcggcac
    13981 gcgcctgctc ggcctgcgcg gcatccagtg cgcgctgggc cgtcaccagc tggccttcgg
    14041 cgttctggcg gttttgccgg gccagatcgg cctcttgccg ggctttttcc gccaggcggg
    14101 tggcgcgctg gtaattcatc tgcgcggcca gcaaatcatc gtcaatggtc tgggcggcgg
    14161 caacagcagc cagcagcgcg ccagccagcg ccagagagcg tgtcagcagc aacatggcaa
    14221 acctcggggt gtaaagtggg atggcgggat gatacccgaa gcgggcaaca agcgtttatc
    14281 atcaagcgct ttacacctgc ttgcagagga tgaggccatg gccgctggcg aactcgaccc
    14341 cggtctgatg atctggcacg gcaaccagct ggaagacctg cgcgatgtgc tggccgcctg
    14401 gctgcgccgc cagcccttgg cgccgctgga aaacgaaatc atcctggtgc aaagccaggg
    14461 catgggccag tggctgaagc tggcgctggc cggcgccgat ggcgtcagcg cggcggtgag
    14521 catgcagctg ccgtcgcagt ttttgtggcg cgcttaccgc gatgtgctgg ggccggatgc
    14581 cgtgccggcc gattcgccgc tggagcggcg caccctggtg tggcgtttga tgcggctgtt
    14641 gccggcctta agcgccgggc cggagtttgc cgtgctgcgc ggttttctgg ccggcgacga
    14701 cgacggccgc aagctgtttc agctggccgg caagctggcg gaactgtttg accaatacca
    14761 agtgtaccgc gccgactggc tggccgactg gcaagccggg cgcgatggcc tggcgcagac
    14821 ggacgggcag tggcgggcgc tgacggacga cgaccgctgg cagccggcgc tgtggcgcgc
    14881 gctgctggcc gatattgccg ccgacggcca tcaggacatc ggccgcgcgc agattcacca
    14941 gcagtttttg caggcgctgg ccgccgcccc gagccgcccg ccggccttgc cgcgccgggt
    15001 ggtggtgttt ggcatttctg ccctgccgca gcaggcgctg gccgcgctgg cggcgctggg
    15061 ccgccactgc caggtgattc tggcggtgca caacccctgc cagcattact ggggcgatct
    15121 ggacgacccg aaaaccatcg cccgccgcct ggcgcggcgc ggcgctggcg cgctgcccag
    15181 ccgcgacagc ctgtacgccg acaaccagcc gctgctggcc gcctggggcc ggcatggccg
    15241 ggattttttg gcgctgctgg accaatacga ccagcccgag cgctatcagc cgcagctggc
    15301 cagccagggc cagcgcattg atttattcac cgagccagac gccagccggc tgctgggcca
    15361 gctgcaacac ggcatttttg atctcacccc gccgccggcc tcgcccgagg cgcgccagcc
    15421 ggtggcggcg gacgatgact cgctgtgttt tcaccttgcc cacagccgcc agcgcgaagt
    15481 ggaaatcctg cacgaccaat tgctggccta ttgcgaggcg gcgcaggctg ccggcgcgcc
    15541 gctggcgccg cgcgatattg tggtgatgac gccggatgtg gccgcctacg cgccgcatgt
    15601 gcgcgcagtg tttggccgca ttccgcccgg cgacccgcgc catttgccgt tcaccctggc
    15661 cgaccggcgc gcgcgcgaca gctcgccgct gttgcgcgcg ctggatgcgc tgctggccag
    15721 cccgcacgcg cgctttaccg tcagcgaggt gtttgacctg ctggacgcgc cagcgctggc
    15781 gcgccgcttt ggcctgcaag ccagccagct gccggccttg cgccagtggg cggaacaggc
    15841 cggcgtgcgc tgggggctgg acgccgacca gcgcgcccaa ctcaatctgc ccgacggcct
    15901 gaaccaaaac agctgggcgt ttggcctgga gcggctgctg ttgggctatg cggtgggcga
    15961 tggcgacgcc tgggatggcc gcatccagcc ctgcgacgag gccgccggcc tggaagtggc
    16021 cgcgctggga ccgctggcgc agctggtgga cgccttgcgc cgctggtcgc gcgtgctggc
    16081 cgagcctgcc gcgcctgcgc aatggggcga gcgcctgcgc cagctgatgg cggatttttt
    16141 gctgccggac gacgaccacg agcggcgcat gctgctgcgg ctggacagcg cgctggaaga
    16201 ctggctgagc gcctgcgccg acgccgggct gcacgccgcg ctgccgctgg cggtggcgcg
    16261 cgaaggctgg ctggccgggg tggacgaagc cgcgccgtcg cagcagtttt tggcgggcgc
    16321 ggtgaatatc tgcacgctga tgccgatgcg ggcgattccg tttcgtgtgg tgtgtttatt
    16381 gggcatgaac gacggcgatt atccgcgccg ccagccgccc agcgactttg acctgatggc
    16441 gcgcccgtat ggccagcgcc ccggcgaccg cgcccgccgc gacgacgacc gctatctgct
    16501 gctggaagcg ctgctgtcgg cgcgcgaacg cctgcacatc agctgggtgg ggcgcagcgt
    16561 gcgcgacaac agcccgctgc cgccgtcgat gctgattggc cagttgcgcg acgcgctggc
    16621 cgccggctgg cggctggccg acgaccccga ggccagcggc gagcgcctgc tggcggcgct
    16681 cagctgcgaa cacccgctgc aaccgttcag cccgcgctat ttcagcggcg aagacccccg
    16741 gctgttcacc tacgccagcg aatggcgcgc cgcccacgac cgcgccgacg ccgcccccac
    16801 ggcgctgccg cccgccacgc cggccgtcac cctggtgggc gtcaccgagc tggcgcggct
    16861 gctgcgccgg ccgactgcgc atttttttca gcgccggctg cgggtgaact ttgccaacgc
    16921 cgacgacagc ctgctggagc acgagccctt cgagctgtct ggcctggatg gctacgcgct
    16981 gcgcgagcgg ctgtttgccg cgctgcggcg cggcgcgcgc gatggcctgt cggccgaggc
    17041 ggtgtgcgcc gccgagctgg cgcgctggca acgcgaaggc gtgctgccgc tgcaagccgc
    17101 cgaggcgcgc tttgccgcgc tggccgagca agtcaccccg gtgtggcaag cctggcaggc
    17161 gcaggccgcg cactgcccgg cgcggcctgc gcgcgaaatc agcctgagcc tggcgcacgg
    17221 cctgcaactg gaagactggg tgcgcgagct gcacgacagc gaggccggcc tgttgcggct
    17281 attggccctg ccgcacccgc tgcgccacag cggcggctgg cgcgccgagc gcctgctggc
    17341 gccgtggctg gcccacctgg cggtgaacgc cgaccacacc ccgctgcgca cctgcctgct
    17401 ggccagcgac gacggcgaac atggcccgct gtgctggccg gccctgcccg cagaacaggc
    17461 gcaaagcctg ctggccagcg tcgccgaggc ctggcgcgcc gggctggatg cgccgctgcc
    17521 gctggcctgc aaaaccacgc tggcctatct gcaataccga ttgcgccacg ccgacgacga
    17581 gccggaggag gttgaagccg ccgcctggca ggccgccagc cagaccttta tcggcggctg
    17641 gaatctgcgc ggcgaagtgg acgacgaccc ctatctggcc cgcgcctacc ccgactggcc
    17701 ggcgctgctg gccggcggcg agcgcgcccg cgactgggcc gagcgcctgt atctgcccct
    17761 gctgcacgcc accctgtcgc ccaaggaatg tttgtgatga gccagccgct gaaccccttg
    17821 accctgccgc tgtccggcag ctggctgatt gaggccagcg ccggcaccgg caaaacctac
    17881 accctggccg cgctgtatgt gcggctggcg ctgggccacg gcccggacga tggcgcgcgg
    17941 ctgccggcgc tgagccccag ccagattctg gtgatgacct ttaccgaagc cgccgccggc
    18001 gagctgcgcg accgcatccg tcagcggctc accggcctgg ccgactggct gcgccgcgac
    18061 gccccggccg ccgaagccga cgccttcagc ctgggtttgc tgggcgagtt ggacgaagcc
    18121 gacggcctgg gccgcgaaca agcggcgcgg cggctggcgc tggtggccga tttgctggac
    18181 gacgcggcga tttccaccat ccacagctgg tgtttgcgca tgctgcgcga gcacgcgctg
    18241 tacagcggca atctgtttga cgaaagcctg gacagcgacg acggcgcgcg ctacgccgac
    18301 agcgtgcgcg actactggcg cacctttgtc tatccgctgt ccgacgccga ctttgccgcc
    18361 gtggccgtgc actgggccac cccggaccaa ctggccgacg ccctgcgccc gctgctgggc
    18421 gaactgcctg agccgcccgc cgtctggcgt gggccgctgg cgctggcgcg cgggcgtcag
    18481 cacagcctgc aagaagccgc cgcgctcaaa accggctggc ctgagcgggt ggcggaactg
    18541 cgcgcgcgct ttgccgactg gcaggcggcc aaggcgtata acggcaaccg cctgcgcgcc
    18601 gccaccctgg ccaaactgct ggacgcgctg gaggcttggg ccagccagcc gcatctggcg
    18661 cagctggacg acagcctgga ctggagccgc ttgtcgccag acttcttggc ggacatctgg
    18721 aaaggcgacg gcccgccgcc gtgcgagccg ctgagcgcgc tggcgacgct gcacacccag
    18781 ctggcggcgc tgacgccgtt tcaccatggc gtgctggcgc acgccagcca gtggctgggc
    18841 catgtgctgg acgcgcacaa gcgccgcgaa gcggtgatgg attttgacga cctgctgatt
    18901 cgcctggacc aggccttgca gaccgacgcc agccaccccg accccgagca gcgcggccgg
    18961 ctggcggcgg ccattcgcca gcagtttccg gcggcgctgg tggatgagtt tcaggacacc
    19021 gacccgcgcc agtaccgcat cctcgatgcg ctgtaccagg tggccgcgcc gcaccccggc
    19081 acgctgctgg cgatgattgg cgacccgaaa caggcgattt acgcctttcg cggcggcgat
    19141 atttttacct acctcaccgc ccgcgccgcc gcccacggcc gccatgtcac cctggacacc
    19201 aatttccgct ccaccgaggc catggtggcg cgggtgaacg ccatgttcgc ccgcgccgag
    19261 gcccgccccg gcggcgcatt tgcctttgcc gacgacgcgg gcgcgccgct attgccgttt
    19321 cagccggtgc gcgccgccgg ccggcgggaa accctgcacc accagggcgc gccgctggcg
    19381 gccttgcagg tgtggctgga cgccgagccg ctgaacgccg ccagctaccg cgaacgcatg
    19441 gccgagcgct gcgccgccca ggtgtgcgcg ctgctggccg gcgcgcaagc cggcgacacc
    19501 ggctttgccg acgccgacgg cggctggcgc gcgctcaccg ccgccgacat cgccatcctg
    19561 gtgcgcgacg gcggcgaggc aaaaagcgtg cgcgcggcgc tgactcgacg cggcatccag
    19621 agcgtgtatc tgtccgaccg cgattcggtg ttcgccagcc gcgaagccgc cgacctcttg
    19681 cactggctgc gcgcctgcgc cgaacccacc caagagcgct gggtgcgcgc cgcgctggcc
    19741 accgccacgc tggacctgcc ctacgcccgg ctggacgcgc aaagcgaggc cggcgcgcgc
    19801 gactgggacg ccgaagtcga acgctttgtc gcgctgaatg cctgctggcg gcggcaaggc
    19861 gtgctgccca tgctgcgcca gctgctgcac cgctttgatg tgccggcgcg ctgcctggcg
    19921 cgcgacggcg gcgaacgcgc gctgaccaac ctcttgcacc tggccgagct tttgcacgcc
    19981 gacgccgagc tgctggacgg cgaacacgcg ctgatccgcc gcctggccga gcgcatcgcc
    20041 cggcccgatc gcggcggcga cgagcgcatt gtccgcctgg aaagcgacgc cggcctggtg
    20101 cgggtggtga ccatccacaa atccaaaggc ctggaatatc cgctggccat gctgccgttt
    20161 gccgccagct accggccggt ggacggcgcg cggctgccgg cctatcgcta tcaccaaaaa
    20221 gacggcgacg cctatcggcc ggcgctgtcg ctgttcaagc gcgaagacga gcgcctggcg
    20281 caagaccgcg agcgcctgca agaagacctg cggctgtttt atgtggcggt ca
//
LOCUS       DAOPYX010000016        20273 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_2745_length_20273_cov_10.970719, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000016 DAOPYX010000000
VERSION     DAOPYX010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 20273)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20273)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20273
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2745_length_20273_cov_10.970719"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2132
                     /gene="carB"
                     /locus_tag="PKN55_01715"
     CDS             <1..2132
                     /gene="carB"
                     /locus_tag="PKN55_01715"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009115808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HNX69992.1"
                     /translation="IPRFAFEKFPQANDRLTTQMKSVGEVMAMGRSIQESMQKALRGM
                     ETGLSGLDERSTDRETIVRELGEPGPERLLYVADAFRIGMSAREIFEVCKIDPWFLAQ
                     LQDLVAEEAAVKGQSLDALDADTLRRLKRKGFSDRRLAALLGVDQGAVRNKRWAAGIR
                     PVYKRVDTCAAEFSTNTAYMYSTYEEECEAQPSSRQKIMVLGGGPNRIGQGIEFDYCC
                     VHAALSLRESGFETIMVNCNPETVSTDYDTSDRLYFEPLTLEDVLEVVNVEKPIGVIV
                     QYGGQTPLKLARALEANGVPIIGTTPDMIDCAEDRERFQKMLHALNLKQPPNRTARDP
                     QEAIRLAAEIGYPLVVRPSYVLGGRAMEIVHEQADLERYMREAVKVSNDSPVLLDRFL
                     NDAIEVDVDAISDGEQVIIGGIMQHIEQAGVHSGDSACSLPPYSLGAEVQDEIRRQTI
                     AMAKALNVVGLMNVQFAIQGDTIYVLEVNPRASRTVPFVSKVTGQPLAKIAARCMAGI
                     TLAAQGVTREVIPPYFAVKEAVFPFIKFPGVDTILGPEMKSTGEVMGVGGSFAEAYVK
                     SQLAAGDRFPKQGKVFLSVRQSDKNGIVEVARDLLDLGFELVATRGTAQMISEQGLPV
                     TAINKVTEGRPHVVDMIKNGEICLVINTVDEKRQAVQDSYSIRRSALQGRIPQYTTLA
                     GARAVCIGMRHLQDLGVYSVQSLHSQL"
     gene            2384..2860
                     /gene="greA"
                     /locus_tag="PKN55_01720"
     CDS             2384..2860
                     /gene="greA"
                     /locus_tag="PKN55_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008952492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HNX69993.1"
                     /translation="MIKVPLTVVGAEQLKAELQHLKSVERPSVIAAIAEARSHGDLSE
                     NAEYDAAKERQGFVEGRIAEIEGKLSNAQIIDPSTLDADGRVVFGATVQLLDMETDDE
                     VRYQIVGDDEADIKLGKVSVNSPIARALIGKEAGDVAEVQAPGGLREYEVLDVQYL"
     gene            2892..3359
                     /locus_tag="PKN55_01725"
     CDS             2892..3359
                     /locus_tag="PKN55_01725"
                     /inference="COORDINATES: protein motif:HMM:NF025046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4149 domain-containing protein"
                     /protein_id="HNX69994.1"
                     /translation="MDERSWNSRLRQLAMTLWIGGQWLAGWGVAPILFARLDTQLAGE
                     LAGRVFTLVEWLGLACALVWLGFYALRHGGAGLRRGEARLVWAMLACSLANQWLIHPV
                     IERLKAMPVDAAGAPAQMLGLGFGPWHGVSSSVYLLQSLLGLAWVLWPRRRGD"
     assembly_gap    3363..3462
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3584..3895)
                     /gene="yhbY"
                     /locus_tag="PKN55_01730"
     CDS             complement(3584..3895)
                     /gene="yhbY"
                     /locus_tag="PKN55_01730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012698344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HNX69995.1"
                     /translation="MELSSDQRSHLKALAHSLNPVVMIGNNGLSEAVMREIAVNLDAH
                     ELIKIRVLGDDREARVAMFEQICDELGAAQVQHIGKLLVIYRPTGDAKIALPKSKKKP
                     A"
     gene            3949..4566
                     /gene="rlmE"
                     /locus_tag="PKN55_01735"
     CDS             3949..4566
                     /gene="rlmE"
                     /locus_tag="PKN55_01735"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008952495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016436 - rRNA (uridine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HNX69996.1"
                     /translation="MARSKTSKAWLQEHVSDPYVHMAQKDGYRSRAAYKLLEIDEKDK
                     LLRPGITVADLGSAPGSWSQVAIDKVGERGRVLALDILPMNPIAGVQFIQGDFREDDV
                     LAQFVQLLEGRPVDLVISDIAPNMSGNSTIDQARSAYLVELALMFAQEHLKPGGHFLV
                     KVFQGSEFNAFLKAMRETFTEVLSRKPKASRDRSSEIYLLGKNRR"
     gene            4682..6589
                     /gene="ftsH"
                     /locus_tag="PKN55_01740"
     CDS             4682..6589
                     /gene="ftsH"
                     /locus_tag="PKN55_01740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017508223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HNX69997.1"
                     /translation="MNNIGKNIAIWVIIGLVLMTVFNQFSKRQETQNQVEYSTFIGDV
                     EAGRVQSVVIEGHPLRGQWLKGRRADGSAFTTYAPYDPQLVNQLIKSNVRFSAKPEEE
                     PSLLMNIFISWFPMLLLIGVWVFFMRQMQGGGKGGAFSFGKSRARMLDQEQNTVTFAD
                     VAGCDEAKEEVKEIVDYLRDPSRYQTLGGRIPRGILLAGSPGTGKTLLAKAIAGEAKV
                     PFFSISGSDFVEMFVGVGAARVRDMFENAKKNSPCIIFIDEIDAVGRQRGAGLGGGND
                     EREQTLNQLLVEMDGFDTNSTVIVIAATNRPDVLDPALQRPGRFDRQVVVPLPDIRGR
                     EQILNVHMRKVPIAVDVKPEIIARGTPGFSGADLANLVNEAALFAARRNKRLVDMEDF
                     ESAKDKIMMGAERRSMVMTDEEKKNTAYHESGHAIVAKLLPKSDPVHKVTIIPRGRAL
                     GVTMQLPEQDRYAYDRDYLLQRISVLFGGRIAEELFMNQMTTGASNDFERATSMARDM
                     VTRYGMSDKLGPMVYGENEGEVFLGRSVTTHKNLSEATMQQVDAEIRRIIDDQYSLAR
                     RLLDENRDKVEAMTAALLEWETIDAEQIEDIMQGRPPRPPRPAGNTGSGNTPPAPPAP
                     ATPTIKPAQEV"
     gene            6656..7501
                     /gene="folP"
                     /locus_tag="PKN55_01745"
     CDS             6656..7501
                     /gene="folP"
                     /locus_tag="PKN55_01745"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011137343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HNX69998.1"
                     /translation="MSAASLHAGRFVLSLDRPLIMGILNVTPDSFSDGGRYQGVAAAR
                     ARAEAMLAEGADMLDVGGESTRPNAPLVSVQEELDRVLPVLEALCELDVPISLDTRRT
                     EVMRAGLALGVDVINDVAALEDDGAIAAVAASSAAVCLMHKQGTPQSMQRAPQYRDVV
                     AEVIDYLGARVHAALAGGIARERLLVDPGFGFGKTVEHNYALLARLGECARLAPVLAG
                     LSRKSMLGAVTGQSEPAARVAASVAAAVLACQHGAAVVRVHDVAATRDALAVWHAMRD
                     AQRAC"
     gene            7636..8979
                     /gene="glmM"
                     /locus_tag="PKN55_01750"
     CDS             7636..8979
                     /gene="glmM"
                     /locus_tag="PKN55_01750"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular transferase
                     activity, phosphotransferases [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HNX69999.1"
                     /translation="MSARKYFGTDGVRGEVGQAPITPDFVLRLGYAAGQVLINHDSGH
                     RPGVLIGKDTRISGYMLEAALQAGFAAAGVDVYLAGPLPTPGIAYLTRALRLQAGVVI
                     SASHNPYQDNGIKFFAEGGVKLDDALELEIEARIDAPMQVRPSAELGRAKRLTDAQGR
                     YIEFCKSTFPNDRDLRGLKLVVDCANGATYHIAPKVFHELGADVIEIATQPNGFNIND
                     HCGATHTKALQQAVLAHDADYGIALDGDGDRLMMVDRAGRLYDGDQLLYVMAKARKRA
                     GQLEGGVVGTVMTNLAMEHALARQGIEFARAKVGDRYVLEQLLARGWQVGGESSGHLL
                     CLDRHTTGDGIISSLQVLTALSVLDTDLAGVCRDWQPFPQTMINVRLQAGMDWQGASA
                     AALAEAETALAGCGRVVLRPSGTEPVVRVMVEAEDKTLAQHWAQVIAQAIDGQVR"
     gene            9238..10662
                     /locus_tag="PKN55_01755"
     CDS             9238..10662
                     /locus_tag="PKN55_01755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008953168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HNX70000.1"
                     /translation="MPSLPPPNPGLYDEMFDGATPRAHCAAYADWLHGLSDEALSARQ
                     READLLFRRHGVTFTLRDNPALDRHIPFDIVPRILPARDWALLERGLRQRLAALNAFL
                     HDIYHDRDILRAGVIPPEQVLTNPQYQVAMHGLDLPNRVYAHVAGVDLARHSDGAFYV
                     LEDNLCVPSGVSYMLESRKMMRRLFPELFARLDIAPVEHYASLLLTTLREASPVAQPT
                     VVVLTPGRHNAAYFEHAFLARQMGVELVEGQDLFVQNDRVWMRTTAGPQTVDVIYRRI
                     DDAFLDPLAFNPDSLLGVPGLLSVYRKGQVALCNAPGAGVADDKSIYPYVPDMIRFYL
                     GQEPLLNNVQTWQLRKPDDLAYVLTRLPELVVKEVHGSGGYGMLIGPLASRAEIAEFR
                     QRILAAPANYIAQPTLALSTCPSVVEAGLAPRHVDLRPFVLSGGDIRIVAGGLSRVAM
                     QAGSLVVNSAQGGGAKDTWIVEGA"
     gene            10662..11573
                     /locus_tag="PKN55_01760"
     CDS             10662..11573
                     /locus_tag="PKN55_01760"
                     /inference="COORDINATES: protein motif:HMM:NF016086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-E domain-containing protein"
                     /protein_id="HNX70001.1"
                     /translation="MLARTAANLYWMSRYLERAENLARVLEVNQGLAWLKAARPGEDM
                     AAPLRLTDSEAAFAAQHGAELTPAALWAFLGFSAHPASIRSCLARARDNADAARDALT
                     GEMWQAIHATWLGMQAAIDAPPADPANFADWVKERSQLFRGITYGAIHRNDAYRFIRL
                     GTFIERADNTARLLKLRYRGHRAGMADHYGLMALLESVSAQDAYLAVYHDTVSAPKVA
                     ELLLLNPDAPRSLAACFAEIRQILPQIGGPRGRAAKKRATELAAALEYGELQDILAHG
                     LQNYLGEFLDQLTQLNDELHAAYMEAA"
     gene            11570..12304
                     /locus_tag="PKN55_01765"
     CDS             11570..12304
                     /locus_tag="PKN55_01765"
                     /inference="COORDINATES: protein motif:HMM:NF019977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase N-terminal domain-containing
                     protein"
                     /protein_id="HNX70002.1"
                     /translation="MKLHISHRSVYHYPERVRHSTQCLRLTPQSSRRQRVLSWQLGLP
                     ARASSGRDPYGNCQHVLTLDYPHQDITVLATGVVEVDEALADDVDDELAPLVFLRQTM
                     RTTPSAARLALAQRHAEAVRQHGRHGALTLARALAEQSGAAKDSVGASHALLGCLRAL
                     AQPARYVSGYLSLADGGEVAHHNWVELWRDGVWASVDPMSLAPAGGGHVKLAVGMDEL
                     DACPIRGLRRPSMEDMLRQAQHSAQQ"
     gene            12398..13129
                     /locus_tag="PKN55_01770"
     CDS             12398..13129
                     /locus_tag="PKN55_01770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012697422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome-type protease"
                     /protein_id="HNX70003.1"
                     /translation="MTYCAALNLKDGLVFASDSRTNAGVDHIATFRKMHLFQQPGERA
                     IVLLNSGNLATTQSVVSLLRARTLHDGEHLLNISSLYDAARLVGETVREVVDRDTGQQ
                     ASNIDFGCSFLVGGQIRGEQPRLFNVYPQGNFIESSTDTPFFQIGESKYGKPILDRVI
                     RYETDMNEALKCLLISFDSTIRSNLSVGLPMETLRYRIDSFAELRPHRIDEGDEYFQQ
                     LRGRWASGLRQVFASLPDPEWFDEA"
     gene            13621..14625
                     /locus_tag="PKN55_01775"
                     /pseudo
     CDS             13621..14625
                     /locus_tag="PKN55_01775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018151007.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
     gene            complement(15266..15823)
                     /locus_tag="PKN55_01780"
     CDS             complement(15266..15823)
                     /locus_tag="PKN55_01780"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018749064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HNX70004.1"
                     /translation="MSTLDPLNYADYIRSRIRTVPDWPQAGVQFRDITPLLQDPKTFR
                     VLVDVFVHRYMDQRIDLIAGLDARGFILGSVVAYELNIGFVPVRKKGKLPYTTVAEEY
                     ELEYGNATVEVHTDACQPGQRVVVLDDLVATGGTMMAGVKLLSRLGGEIVEAGAIVDL
                     PELGGSQLIRQAGIPLFTLCDFAGH"
     gene            16039..16662
                     /gene="gmk"
                     /locus_tag="PKN55_01785"
     CDS             16039..16662
                     /gene="gmk"
                     /locus_tag="PKN55_01785"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012695943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HNX70005.1"
                     /translation="MQTGNIFIVTAPSGAGKTTLVAALLAADKDVQLSVSHTTRQPRA
                     GEVHGEHYHFVDRATFEAMIARKDFLEYAEVYGNYYGTSLRWLREQLSLGRDILLEID
                     WQGAQQVRKAFPQAVGIFILPPSVEALGERLRGRAKDSEATIEARLAVVRSEIDHAEE
                     ADYLIINEQLDDAVRDLIAVVRAQRLRTARQMQARGEQVRAMQSQAR"
     gene            16718..16924
                     /gene="rpoZ"
                     /locus_tag="PKN55_01790"
     CDS             16718..16924
                     /gene="rpoZ"
                     /locus_tag="PKN55_01790"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008952512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HNX70006.1"
                     /translation="MARITIDDCLKRIPNRFDLTLAATYRARQLASGATPLVEAGKDK
                     PTVLALREVANGHVGREMLDRHRG"
     gene            16961..19150
                     /locus_tag="PKN55_01795"
     CDS             16961..19150
                     /locus_tag="PKN55_01795"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012695945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HNX70007.1"
                     /translation="MSSDVNERIDYDALVANEAAQLLSAARAYVKPDDLPLIRQAFDF
                     SRDAHEGQVRKSGEPYITHPLAVAHILTDWKMDAHGLAAALLHDTMEDTGVAKSTLEE
                     RFGSVVAELVDGLSKLERLEYQTKETAQAENFRKMVLAMAKDIRVIIVKLADRLHNMR
                     TLDAMREDKRRRIAQETIDIYAPVANRIGLNKVYRELQDLCFKHLHPNRYQVLHKAIR
                     AARGNRRELVSKIMQALSQKLVQANIEASIKGREKNLYSIYHKMQEKQLSFSEVLDIY
                     GFRVIVNDIPSCYLALGAMHALYKPIPGKFKDYIAIPKSNGYQSLHTTLFGPYGTPIE
                     VQIRTLDMHHIAEAGVASHWMYKSGDESIDKAQQRTHQWLQEILELQAGSGDAIEFLE
                     HVKVDLFPDEVYVFTPKGRILVLPQGSTPVDFAYAVHTDIGHRCIAAKINHELAPLRT
                     PLKNGDQVEVITTTQSKPNPAWLSFVASGRARSHIRNYLKSLERDEAAHLGERLLKQA
                     VQALATRSLSVDADTWQAYLREFAEKGQKSSDVLMEIGIGKQLPMVVASRLLELAGEA
                     LGEDVRAGPVMIRGNEGAAVQFAACCNPIPGDRIAGVINKGQGLMIHAHDCPTLGRID
                     HDRQLEVEWDLVEPRLFGVPITVLAYSERGALAAVAAAITEAHANIESVDMQNAHTGE
                     GAIQIRFRLQVENILHLADVLANIQEQPTVVRAVRGILSEPAASKGH"
     gene            19256..20221
                     /locus_tag="PKN55_01800"
     CDS             19256..20221
                     /locus_tag="PKN55_01800"
                     /inference="COORDINATES: protein motif:HMM:NF016629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4351 domain-containing protein"
                     /protein_id="HNX70008.1"
                     /translation="MDADDYDNPWKQAIDEYFADFIACYFPQAHQDIDWAAGYETLDK
                     ELPGLARDADLGQRYADKLVKVRTRQGQHDLVYVHIEVQGQHDPDFAQRMFVYHYRIF
                     DRFGQPPVSLAVLADEHPHWKPSRYGYQRWGCALEFAFPTVKLSEHTQSLDALLASDN
                     PFAILTAAHWLTRRSKRNMDERLASKLRLIKLLYHKGWTRQAVLDFFAVIDWMMHLPE
                     ALTQQFRQQLADYEQENIMRYVTSVERQAREEGVLTGLQQGMQQGIQQGEAAFLLRLL
                     NRRFGALPAEWVLRVQQASHAELELWGERVLDAASLDEVFAPQTH"
BASE COUNT         3770 a         6435 c         6364 g         3604 t
ORIGIN      
        1 agattccgcg ttttgccttc gagaaattcc cgcaggccaa cgaccgcctg accacgcaaa
       61 tgaagtcggt gggcgaagtg atggccatgg gccgttcgat tcaggaatcc atgcaaaaag
      121 ccctgcgcgg catggaaacc ggcctgtccg gcctggacga gcgctcgacc gaccgcgaaa
      181 ccatcgtccg cgaactgggc gagccggggc cggaacgcct gctgtacgtg gccgatgcct
      241 tccgcattgg catgagcgcg cgcgaaatct tcgaagtgtg caagatcgac ccgtggttcc
      301 tggcccagct gcaagacctg gtggccgaag aagccgccgt caagggccaa tccctggacg
      361 cgctggacgc cgacaccctg cgccgcctga agcgcaaggg cttctccgac cgccgtctgg
      421 ccgcgctgct gggcgtggat cagggcgcgg tgcgcaacaa gcgctgggcc gccggcatcc
      481 gcccggtgta caagcgcgtg gacacctgcg cggccgagtt ctccaccaac accgcctata
      541 tgtactcgac ttacgaagaa gagtgcgagg cgcaaccgtc cagccgccag aaaatcatgg
      601 tgctgggcgg cgggccgaac cgcatcggcc agggcattga attcgactac tgctgcgtgc
      661 acgccgcgct gagcctgcgc gaatccggct ttgagaccat catggtcaac tgcaacccgg
      721 aaaccgtgtc caccgactac gacacttctg accgcctgta cttcgagccg ctgacgctgg
      781 aagacgtgct ggaagtggtg aatgtggaaa aacccatcgg cgtgatcgtg cagtacggcg
      841 gccaaacccc gctgaaactg gcccgcgcgc tggaagccaa cggcgtgccc atcatcggca
      901 ccacgccgga catgatcgac tgcgccgaag accgcgagcg cttccagaag atgctgcacg
      961 cgctcaacct caagcagccg cccaaccgca ccgcgcgcga cccgcaggaa gccatccgcc
     1021 tggccgctga aatcggctac ccgctggtgg tgcgcccgtc ctacgtgctg ggcggccgcg
     1081 ccatggaaat cgtccacgag caggccgacc tggagcgcta tatgcgcgag gcggtgaagg
     1141 tgtccaacga cagcccggtg ctgctggacc gcttcctgaa cgacgccatc gaagtggacg
     1201 tggacgccat cagcgacggc gaacaggtga tcatcggcgg catcatgcag catatcgagc
     1261 aagccggcgt gcactctggc gactccgcct gctcgctgcc gccgtacagc ctgggcgctg
     1321 aggtgcagga cgaaatccgt cgccagacca tcgccatggc caaggcgctg aacgtggtgg
     1381 gcctgatgaa tgtgcagttt gccattcagg gcgacaccat ctacgtgctg gaagtcaacc
     1441 cgcgcgcctc gcgcaccgtg ccgtttgtct ccaaggtcac cggccagccg ctggccaaga
     1501 tcgccgcgcg ctgcatggcc ggcatcaccc tggccgccca gggcgtgacc cgcgaggtga
     1561 ttccgccgta cttcgccgtc aaagaagcgg tgttcccgtt catcaagttc ccgggcgtgg
     1621 acaccattct tggcccggaa atgaaatcca ccggcgaagt gatgggcgtg ggcggctcgt
     1681 ttgccgaagc ctacgtcaag agccagctgg cggccggcga tcgcttcccg aaacagggca
     1741 aggtgttcct gtcggtgcgc cagtcggaca agaacggcat tgttgaagtg gcccgcgacc
     1801 tgctggacct gggctttgag ctggtggcca cccgtggcac cgcgcaaatg atcagcgaac
     1861 agggcctgcc ggtgaccgcc atcaataaag tcaccgaagg ccgtccgcat gtggtggaca
     1921 tgatcaagaa tggcgaaatt tgtctggtga tcaacaccgt ggacgaaaag cgtcaggcgg
     1981 tgcaagacag ctattccatc cgccgctcgg cgctgcaagg ccgcattccg cagtacacca
     2041 ccctggccgg cgcgcgcgcc gtgtgcattg gcatgcgcca cctgcaagac ctgggcgtgt
     2101 acagcgtgca gtcgctgcac agccagctgt aattgcctgc actgccgggc ctgcggcccg
     2161 gcggtttcac ggcggcgcgc gcctggcgtt ttgccatcgg cgcgcgtttg acttcccgat
     2221 gacatgttct ggcggttttc gcacttggaa accgccgggc ttgccgctac aatcggggca
     2281 ttccgttcta cagacgaacc ttcgcaagcc gccgtttttc aacggcggct tgccttttat
     2341 tgtgtggaaa ccggcctgtc gccggcttcc ctggagaagc aaaatgatca aagttccgct
     2401 gaccgtggtg ggcgccgagc agctcaaggc cgagctgcaa cacctgaaaa gcgtggagcg
     2461 tccgagtgtg atcgccgcca tcgccgaagc gcgcagccat ggcgacctgt cggaaaacgc
     2521 cgaatacgac gccgccaaag agcgtcaggg ctttgtggaa ggccgcatcg ccgaaatcga
     2581 aggcaagctg tccaatgcgc aaatcatcga cccgtccacc ctggacgccg atggccgcgt
     2641 ggtgtttggc gccaccgtgc agctgctgga catggaaacc gacgacgaag tgcgctacca
     2701 gatcgtcggc gacgacgaag ccgacatcaa actgggcaaa gtgtcggtca attcgccgat
     2761 tgcccgcgcg ctgattggca aagaagccgg cgacgtggcc gaagtgcaag cgccgggcgg
     2821 cctgcgtgaa tacgaagtgc tggacgtgca atatctgtaa ctgagcagcc cccactccag
     2881 gagcctgggc aatggatgaa cgcagctgga acagccgctt gcgccaactg gcgatgacgc
     2941 tgtggattgg cggccaatgg ctggccggct ggggcgtggc ccccattctg tttgcccggc
     3001 tggacaccca actggccggc gagctggccg ggcgggtgtt caccctggtg gagtggctgg
     3061 gcctggcctg cgcgctggtg tggctggggt tttacgcctt gcgccacggc ggcgccgggc
     3121 tgcggcgcgg cgaagcccgg ctggtgtggg ccatgctggc ctgctcgctg gccaatcagt
     3181 ggctgattca cccggtgatc gagcggctca aggccatgcc ggtggacgcc gctggcgcgc
     3241 cagcgcagat gctcggcctg ggctttggcc cctggcatgg cgtgtccagc agcgtgtatt
     3301 tgctgcaaag cctgttgggt ttggcctggg tgctgtggcc gcgccgccgg ggcgattgag
     3361 cgnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngcctggcg tcagcctgct
     3481 gtgcataaaa aaagcggcga ggtgagctcg ccgcttttta cttgcctgaa atcccccgcg
     3541 aaaatcgcgt cattcccgcc tgagcgggaa ttcagggggt tgatcaggcg ggttttttct
     3601 tgcttttggg caaggcgatt ttggcgtcgc cggtcgggcg ataaatcacc agcagcttgc
     3661 caatgtgctg cacctgggcg gcgcccagtt cgtcgcaaat ttgctcgaac atggccacgc
     3721 gggcttcgcg gtcgtcgccc agcacgcgga ttttgatcag ttcgtgggcg tcgaggttga
     3781 cggcgatttc acgcatcacc gcttcgctca gaccgttgtt gccaatcatc accaccgggt
     3841 taagcgaatg ggccagcgct ttcagatgac tgcgctgatc agaagagagt tcgattttca
     3901 tcgtattcgt tgtctataaa gaacagtttc aaaacacgag tttactgtat ggcacgcagc
     3961 aaaaccagca aggcatggtt gcaagagcat gtaagcgacc cttatgtgca tatggcgcaa
     4021 aaggatggct accgttcgcg cgccgcctac aaattgttgg aaatcgacga aaaagacaaa
     4081 ctgctgcgcc ccggcatcac cgtggccgac ctgggttcgg cgccgggcag ctggtcgcag
     4141 gtggcgattg acaaggtggg cgaacgcggc cgcgtgctgg cgctggacat cctgccgatg
     4201 aatccgattg ccggcgtgca gttcattcag ggcgattttc gtgaggacga cgtgctggcg
     4261 cagtttgtgc aactgctgga aggtcggccg gttgaccttg taatctccga cattgccccc
     4321 aacatgtctg gcaatagcac cattgatcag gcgcgcagcg cctatctggt ggagctggcc
     4381 ctgatgtttg cccaggaaca cctgaaacct ggcggacatt ttttggtcaa ggtgtttcag
     4441 ggcagcgaat tcaatgcttt cctgaaggcg atgcgagaaa cctttaccga ggtgctcagt
     4501 cgcaaaccca aagcctcacg cgaccgcagc agcgaaatct acctgctggg caaaaatcgg
     4561 cgctgacagg ctgcggttca gggtttacaa tgaaaaaggc gctgtttctg atgaggtttt
     4621 tctgccagga aacagcccag ctatattttt tggcaggcca gggcacagta aggagtccgc
     4681 tttgaacaac atcggtaaaa acatcgccat atgggtcatt atcggcctcg tgctgatgac
     4741 ggtgttcaac cagttcagca agcgtcaaga gacgcaaaac caggtcgaat attccacctt
     4801 cattggcgat gtcgaagcgg gccgcgtgca gtccgtggtg attgagggcc accccttgcg
     4861 cgggcagtgg ctcaagggcc gccgcgctga cggcagcgcc ttcaccacct acgcccccta
     4921 cgatccgcag ctggtcaacc agctgatcaa gagcaatgtg cgcttctcgg ccaagccgga
     4981 agaagagccg tcgctgttga tgaatatctt catctcctgg ttcccgatgc tgctgttgat
     5041 tggcgtgtgg gtgttcttca tgcgtcagat gcagggcggc ggcaagggcg gggcgttcag
     5101 cttcggcaag agccgcgcgc gcatgctgga tcaagaacaa aacaccgtca cctttgctga
     5161 cgtcgccggt tgcgacgaag ccaaagaaga agtgaaggaa atcgtcgatt acctgcgtga
     5221 ccccagccgt tatcaaacgc tgggcgggcg catcccgcgc ggcatcctgc tggccggcag
     5281 ccccggcacc ggtaaaaccc tgctggccaa ggccattgct ggcgaagcca aggtgccgtt
     5341 cttctcgatt tccggctcgg actttgtgga aatgttcgtc ggcgtcggcg ccgcccgcgt
     5401 gcgcgacatg ttcgagaacg ccaagaaaaa ctcgccgtgc atcatcttca tcgatgaaat
     5461 cgacgcggtg ggtcgccagc gcggcgccgg cctgggcggc ggcaacgacg agcgcgaaca
     5521 aacgctgaac cagctgctgg tggaaatgga cggctttgac accaattcca cggtgatcgt
     5581 gatcgccgcc accaaccgcc cggacgtgct cgacccggcg ctgcaacgcc cgggccgctt
     5641 cgaccgccag gtggtggtgc cgctgccgga cattcgcggc cgcgaacaga tcctcaatgt
     5701 gcacatgcgc aaggtgccga ttgcggtgga cgtcaaaccg gaaatcatcg cgcgcggcac
     5761 gccgggcttc tctggcgccg atctggccaa cctggtcaac gaagccgcgc tgtttgccgc
     5821 ccgccgcaac aagcgtctgg tggatatgga agacttcgaa tccgccaagg acaaaatcat
     5881 gatgggcgcc gagcgccgca gcatggtgat gaccgacgag gaaaagaaaa acaccgctta
     5941 ccacgagtct ggccacgcca ttgtcgccaa gctgctgccc aagtctgacc cggtgcacaa
     6001 agtcaccatc atcccgcgcg gccgcgcgct gggcgtgacc atgcagctgc cggagcaaga
     6061 ccgctacgcc tacgaccgtg actatctgtt gcagcgcatc tcggtgctgt tcggcggccg
     6121 cattgccgaa gagctgttca tgaaccagat gaccactggc gccagcaacg acttcgagcg
     6181 cgccacctcg atggcccgcg acatggtcac ccgctacggc atgagcgaca agctcggccc
     6241 gatggtgtac ggcgaaaacg aaggcgaagt gtttctgggc cgttcggtga ccacgcacaa
     6301 aaacctgtcg gaagccacca tgcagcaggt ggacgcggaa atccgccgca tcatcgacga
     6361 tcagtacagc ctggcccgtc gcctgctgga tgaaaaccgc gacaaggtgg aagcgatgac
     6421 cgccgcgctg ctggaatggg aaaccatcga cgccgagcag atcgaagaca tcatgcaagg
     6481 ccgtccgccg cgcccgccgc gtccggctgg caacaccggc tcgggcaaca cgccgccggc
     6541 gccgcccgcc ccggccacgc ccaccatcaa accggcgcag gaagtctgat tctttctcgc
     6601 catcccctcg cgcggcagct tcggctgccg cgccgctttt tatggagctg aaaacatgtc
     6661 cgccgcttcc ctgcatgccg gccgctttgt cctgtcgctg gatcgcccct tgatcatggg
     6721 cattctgaat gtcaccccgg attcgttctc ggatggcggc cgctatcagg gcgtggccgc
     6781 cgcccgcgcc cgcgccgagg ccatgctggc cgaaggcgcc gacatgctgg atgtgggcgg
     6841 cgaatccacc cgcccgaatg cgccgctggt gtcggtgcag gaagagctgg atcgggtgct
     6901 gccggtgctg gaagcgctgt gcgagctgga tgtgccgatt tcgctggaca cccgccgcac
     6961 cgaggtgatg cgcgccggcc tggcgctggg ggtggacgtg atcaacgacg tggcggcgtt
     7021 ggaagacgac ggcgcgattg ccgccgtggc ggcttcgtcc gccgccgtgt gcctgatgca
     7081 caagcaaggc acgccgcaga gcatgcagcg cgcgccgcag taccgcgatg tggtggccga
     7141 ggtgattgat tacctgggcg cgcgcgtgca cgccgcgctg gctggtggca ttgcccgcga
     7201 acgcctgctg gtggaccccg gctttggctt tggcaaaacc gtcgagcaca actatgcctt
     7261 gctggcgcgc ctgggcgagt gcgcccgtct ggcgcccgtg ttggcggggc tgtcgcgcaa
     7321 atccatgctc ggtgcagtca cgggccagag cgagccagcg gcgcgggtgg cggccagtgt
     7381 ggcggcggcg gtgctggcgt gccagcatgg cgcggcagtg gtgcgcgtgc acgatgtggc
     7441 cgccacccgc gatgccttgg cggtgtggca tgcgatgcgc gacgcgcagc gcgcgtgctg
     7501 agcattgtgc gtcttggcaa aactttaatg cttacggcat ggcgtggaac gggtagaatc
     7561 aggacatcat cccggcgcgc gccatgctga gccctcagcc tgcctgcgcg ccctgcatca
     7621 cctcaagagg acacgatgag cgcacgcaaa tacttcggca ccgatggcgt tcgcggtgaa
     7681 gtcggccaag cgccgattac cccggacttt gtcttgcggc tgggctacgc cgccggccag
     7741 gtgttgatca accacgacag cggccaccgc cctggcgtgt tgattggcaa agacacccgc
     7801 atttctggct atatgctgga agccgcgctg caagccggtt ttgccgccgc tggcgtggat
     7861 gtctatctgg ctggcccgct gcccacgccg ggcatcgcct acctgacccg cgccctgcgt
     7921 ttgcaggccg gtgtggtgat ttccgcctcg cacaacccct atcaggataa tggcatcaaa
     7981 ttttttgccg aaggcggcgt caagctggac gacgcgctgg agctggaaat cgaagcgcgc
     8041 atcgacgcgc cgatgcaagt gcgcccctcg gccgagctgg gccgcgccaa gcggctgacc
     8101 gacgcccagg gccgctatat cgagttttgc aaaagcacct tccccaacga ccgcgacctg
     8161 cgcggcctga agctggtggt ggactgcgcc aacggcgcca cctatcacat tgcgcccaaa
     8221 gtgtttcacg agctgggcgc cgacgtgatc gaaatcgcta cccaacccaa cggctttaat
     8281 atcaacgacc actgcggcgc cacccacacc aaggcgctgc aacaggcggt gctggcgcac
     8341 gacgccgact atggcattgc cctggacggc gatggcgacc ggctgatgat ggtggaccgc
     8401 gctggccggc tgtacgacgg cgaccagctg ctgtacgtga tggccaaggc gcgcaagcgc
     8461 gccggccagc tggaaggcgg cgtggtcggc acggtgatga ccaatctggc catggaacac
     8521 gcgctggcgc gccagggcat cgaatttgcc cgcgccaagg tcggcgaccg ttatgtgctg
     8581 gagcaattgc tggcgcgcgg ctggcaggtg ggcggcgaaa gctctggcca cttgctgtgc
     8641 ctggaccgcc acaccactgg cgacggcatt atttccagcc tgcaagtgct caccgcgctg
     8701 tcggtgttgg acaccgacct ggccggcgtg tgtcgcgact ggcagccctt cccgcagacc
     8761 atgatcaatg tccgtctgca agccggcatg gactggcaag gcgcgtcggc ggcggcgttg
     8821 gccgaagccg aaaccgcgct ggccggctgc ggccgcgtgg tgttgcgccc cagcggcacc
     8881 gagccggtgg tacgggtgat ggtcgaggct gaagacaaaa ccctggccca gcactgggcg
     8941 caggtgattg cccaggcgat tgacggccag gtgcgttgag tagctttctc ttctgatcgc
     9001 gtcgcacggt ttgcggccac gcgcttggcc gtgagtgctg tactggggtg agtggtgctt
     9061 ggcgcatctc atcttcaggt attgcgcctt gccgcgcggc gtatccggta caacgcgcac
     9121 cattatggtg cgcgttggcg cattcagcgc catccgcagc gtattgtccg ggcgtgccag
     9181 catggcacac cccttgctga atatcgggct gatgcttgtt gcccggaatg cgccgccatg
     9241 cccagcctgc ccccgcccaa ccccgggctt tatgatgaaa tgttcgacgg cgccacgccg
     9301 cgcgcccatt gcgccgccta cgctgactgg ctgcatggcc tgtcggacga ggcgctgtcc
     9361 gcccgtcagc gcgaggctga tctgctgttt cgccgccatg gcgtcacctt caccctgcgc
     9421 gacaacccgg cgctggaccg tcacatcccc tttgacatcg tgccgcgcat tttgcccgcg
     9481 cgcgactggg cgctgctgga gcgcggcctg cgccagcgtc tggcggcgct gaacgccttt
     9541 ttgcacgaca tctaccacga ccgcgacatc ctgcgcgccg gggtgattcc gcccgagcag
     9601 gtgctgacca atccgcaata ccaagtggcc atgcacgggc tggacttgcc caatcgggtg
     9661 tacgcccatg tcgccggggt ggacctggcg cggcatagcg acggcgcgtt ttatgtgctg
     9721 gaagacaatc tgtgcgtgcc gtccggcgtc agctatatgc tggaaagccg caagatgatg
     9781 cgccgcttgt ttcccgagct gttcgcccgg ctggacatcg ccccggtgga gcactacgcc
     9841 agcctgctgc tgaccacgct gcgcgaagcc agcccggtgg cgcagcccac cgtggtggtg
     9901 ctcacccccg gccggcataa cgcggcttat tttgagcatg cgtttctggc gcggcaaatg
     9961 ggcgttgagc tggtggaagg ccaggatttg tttgtgcaaa acgaccgggt gtggatgcgc
    10021 accaccgccg gcccacagac ggtggacgtg atttaccggc gcatcgacga cgcgtttctg
    10081 gacccgctgg cgttcaaccc cgattcgctg ctgggcgtgc ccggcctgtt gtcggtgtac
    10141 cgcaagggcc aggtggcgct gtgcaatgcg cccggcgccg gggtggccga cgacaaatcg
    10201 atttacccct atgtgccgga catgatccgc ttttatctgg gccaggagcc cttgctgaac
    10261 aatgtgcaaa cctggcagct gcgcaagccg gatgatctgg cctatgtgct gacccgcctg
    10321 ccggagctgg tggtgaaaga agtgcacggc agcggcggct acggcatgct gattggcccg
    10381 ctggccagcc gcgccgagat tgccgaattc cgtcagcgca ttctggccgc gccggccaat
    10441 tacatcgccc agcccactct ggcgctgtcc acctgcccca gcgtggtcga ggctggcctg
    10501 gcgccgcgcc atgtggattt gcgcccgttt gtgctcagcg gcggcgatat ccgcattgtc
    10561 gccggcgggc tgagccgggt ggcgatgcag gccggctcgc tggtggtgaa ttccgcccag
    10621 ggcggcggcg ccaaagacac ctggattgtg gagggcgcgt gatgctggca agaaccgcag
    10681 ctaatttgta ctggatgtcg cgctatctgg agcgcgcaga aaacctggcg cgcgtgctgg
    10741 aggtcaatca aggcctggcc tggctgaagg ccgcccgccc cggcgaggac atggccgcgc
    10801 cgctgcggct gaccgacagc gaggcggcgt ttgccgcgca gcacggcgca gagctcaccc
    10861 cggcggcgct gtgggcgttt ttggggtttt ctgcgcaccc ggccagcatc cgcagctgcc
    10921 tggcgcgcgc ccgcgacaac gccgacgccg cccgcgacgc gctgaccggc gaaatgtggc
    10981 aggccattca cgccacctgg ctgggcatgc aggctgccat cgacgcgccg ccggccgacc
    11041 cggccaactt tgccgactgg gtgaaagaac gctcgcagct gtttcgcggc atcacctatg
    11101 gggcgattca ccgcaacgac gcttatcggt ttattcgcct gggcactttt atcgagcgcg
    11161 ccgacaacac cgcccgcctg ctcaagctgc gctatcgcgg ccaccgcgcc ggcatggcgg
    11221 atcactacgg gctgatggcg ctcttggagt cggtcagcgc ccaggatgcc tatttggcgg
    11281 tgtaccacga cacggtcagc gcgcccaagg tggccgagct tttgctgctt aacccggacg
    11341 cgccgcgcag cctggcggcg tgttttgccg aaatccgcca aatcctgccg caaatcggcg
    11401 gcccgcgcgg ccgcgccgcc aaaaagcgcg ccaccgagct ggccgccgcg ctggaatacg
    11461 gcgaactgca agatattctg gcgcacggcc tgcaaaatta tctgggcgag tttctcgacc
    11521 agctgacgca attaaacgac gaactgcatg ccgcatatat ggaggccgca tgaaactgca
    11581 catcagccac cgcagcgtct atcactaccc ggagcgggtg cgccacagca cccaatgcct
    11641 gcggctgacg ccgcaaagct cgcgccgcca gcgcgtattg tcctggcagc tgggcctgcc
    11701 ggcgcgcgcc agcagcgggc gtgacccgta cggcaattgc cagcatgtgc tgacgctgga
    11761 ttatccccac caggacatca ccgtgctggc caccggcgtg gtggaggtgg acgaagcgct
    11821 ggccgatgat gtggacgacg agctggcccc gctggtgttt ttgcggcaaa ccatgcgcac
    11881 cacgcccagc gccgcgcggc tggcgctggc ccagcgccac gccgaggccg tgcgccagca
    11941 tggccgccat ggcgcgctga ccctggcgcg ggcgttggcc gaacagtccg gcgccgccaa
    12001 agacagcgtc ggcgccagcc atgcgctgct gggctgcctg cgcgcgctgg cgcaaccggc
    12061 ccgctatgtc agcggctatc tgtcgctggc cgacggcggc gaggtggcgc accataactg
    12121 ggtggagctg tggcgggatg gcgtctgggc cagcgtggac cccatgtcgc tcgccccggc
    12181 cggcggcggc catgtcaaac tggcggtggg catggacgag ctggacgcct gcccgattcg
    12241 cggcctgcgc cggcccagca tggaagatat gctgcgacaa gcgcaacact cggcgcagca
    12301 ataaccgcca aatcgttcat catgctgtgt ttgtttcggg atttcccgga taatgccgcg
    12361 acttttttat taacccaacc agactcgcca cttcgccatg acttattgcg ccgccctgaa
    12421 cctgaaagac ggcttggtgt ttgcctcgga ttcgcgcacc aatgccggcg ttgatcacat
    12481 cgccaccttc cgcaagatgc atctgtttca gcagccgggc gagcgcgcca tcgtgttgct
    12541 gaactccggc aacctggcca ccacgcaatc ggtggtcagc ctgctgcgcg cccgcacctt
    12601 gcacgatggc gagcatctgc tgaatatcag cagcctgtac gacgcggcgc ggctggtggg
    12661 cgaaaccgtg cgagaagtgg tggaccgcga caccggccag caggcgtcca atatcgactt
    12721 tggctgttct ttcctggtgg gcgggcagat tcgcggcgag cagccgcgtt tgttcaatgt
    12781 ctatccgcag ggcaatttta ttgagtcgtc caccgacacg ccgtttttcc agattggcga
    12841 atccaaatac ggcaagccga ttctggaccg ggtgattcgc tacgaaaccg atatgaacga
    12901 ggcgctgaag tgcctgctga tttcgtttga ttcaaccatc cgcagtaatt tgtcggtcgg
    12961 cttgccgatg gaaaccctgc gctatcgcat cgacagcttt gccgagctgc gcccgcaccg
    13021 catcgacgaa ggcgacgaat acttccagca actgcgcggc cgctgggcca gcgggctgcg
    13081 tcaggtgttt gcttcgctgc cggatccaga atggtttgac gaggcctgag cgtaggtagt
    13141 gtggcgcgtg cgtcacacgc tcagctggcg cgctggcctt cccttggcga tgtgtctatc
    13201 atgagactgg ctacgtagga atacggttga tgatggcatg ggccaagcgc cgctcacaca
    13261 acccgcctgg ggttgttgcc cgtgtctgct tgatctgtgc gcgcctggcc tggcgcgctg
    13321 ggctgagtgt gctgcgcggc gccaagccgg gttgagttga gctttcaccg catagcgcgt
    13381 gcgacctgac ggtctggcat gctataacgg caacatgctg cccgcttgtc attgactgtc
    13441 aggtaaaatc ctgcctggca gtcattggat ggcgacacgg atgacgcgcc atttcagctt
    13501 tgaattatga aaaaagaata acgggtgggc gtaaaaaagc gcggcaattc aagttgtgta
    13561 aagttgccgc cgataacatg ccaactgtcc cttgacctta gctaaacaag aaccgcactc
    13621 atgctctatt ctctcattcg ccccctgctg ttccggctgg atgctgaaac cgcgcacaat
    13681 gtgaccttgg gcaccttgcg gttccttaac gactacggtc tggacaccct gctcaacaag
    13741 cccattccgg ctcagcccat tgaagtgatg gggctgacct tccccaatcc ggtgggcctg
    13801 gccgctggcc tggacaagaa cggcgactat ctggacgcgc tggctgcgct gggttttggc
    13861 tttgtcgaaa tcggcaccgt caccccgctg ccgcagccgg gcaaccccaa gccgcgcctg
    13921 ttccgctatc cggaagccga agccatcatt aatcgcatgg gctttaacaa cgatggcgtg
    13981 gacgccttgg tggacaacat caagcgctca aggttccagg gcgtgctcgg catcaatatc
    14041 ggcaagaacg cgctcacgcc gatggagcgc gcggtggacg actacctcac ctgcctgaac
    14101 aaggtgtatc tgtacgccag ctacgtcacc atcaatatct cgtcgcccaa caccaaaaac
    14161 ctgcgcgacc tgcaaagctc gaccgcgctg tccgagctgc tgggccgctt gaagatgcgc
    14221 cagctggagc tggcccaaca gcatggccgc tatgtgccgc tggtggtcaa gctcgccccg
    14281 gatctggaag atgaggcaat tgacgacatt gccaaggtgc tgctggccaa ggaaatcgac
    14341 ggcgtgatcg ccaccaacac cacgctgtcg cgcgatggcg tgcagcatat tccgcaagcc
    14401 gcccaggatg gcggcctgtc cggtgcgccg ctgaaggggc gctccacccg ggtgattcgc
    14461 cgcctgtccg acgcgctgga cggcgccatc cccatcattg gcgtgggcgg cattacccgt
    14521 ggcggcgacg cctgggaaaa gctcaacgcc ggtgcgtcgc tggtgcagat ttacagcggt
    14581 ctgatctttc agggcccgca actgatcgaa gccgcggtca atgcctgccg gccgtatttt
    14641 attacccacg gcgtcacccc gcgtcgtcgt ggcccggatg tgcaagcctc caccctgacg
    14701 ccaggtgaga cgcctgcggc agaaacaccc gcggccgacg cgcaaacccc ggcaactgat
    14761 gctgctacgc ccactgtcgc caatcctgag ggggcggtct cttcgcccaa tgaaggcgat
    14821 gccaccgcgc cgccggccaa tcatggcgtg atccagatta cgccgggggt tgagccgccg
    14881 acggtggacg agccgtttac cgaacctgct ccggccacac ctgtcgccgc atcggttgag
    14941 ccgcccaaga agatgacgct ggatcagatg ttggctgccg ccgtggcgcc agtggttccg
    15001 ccgcgcccgc agccggatgc cccggcggcg gatgctggcg ctgaaccggc gcctgtcgcc
    15061 gccgccgcgc cggccgagcc gacggcagat gcgtcgcaag cgcccacgat catcgccacc
    15121 accaaggccg ccaacgccta tatggccagc ggcccgcgca agaaacgccg cccgacctga
    15181 gcgcctgggc gcgcaaccta aaaacaaagc cggcacggtg agtagacacc gtgccggctt
    15241 tgttttgtgc tgttttccgc cgcgctcagt ggccggcaaa gtcacacagg gtgaacagcg
    15301 gaatgccggc ctggcgaatc agctgcgagc cgcccagttc gggcaggtcg acaatggcgc
    15361 cggcttcgac aatctcgccg cccagccgcg acagcaactt caccccggcc atcatcgtgc
    15421 cgccagtggc caccagatcg tccagcacca ccacgcgctg gccgggctgg caggcgtcgg
    15481 tgtgcacttc gacggtggcg ttgccgtatt ccagctcgta ttcttccgcc accgtggtgt
    15541 agggcagctt gccctttttg cgcaccggca caaagccgat gttcagctca tacgccacca
    15601 ccgagccgag aataaaaccc cgcgcgtcca ggccggcgat caaatcaatg cgttggtcca
    15661 tatagcggtg cacaaacaca tcgaccagca cgcgaaaggt ttttgggtct tgcaacagcg
    15721 gggtgatgtc gcggaactgc acgccggctt gcggccagtc gggcacggtg cggatgcgcg
    15781 agcggatgta atcggcgtaa ttcagcgggt caagcgtgga catgaaaatt cctggcaaac
    15841 gataaaacag ggcggattat gcggcgtttg cacaaaaaca cgccagggca aaactgcgcc
    15901 aatttgactg cttggttgac cattccaccg gacatggcgc cacggatagg tgcgcagcgg
    15961 cttggtcgtg gtccaattca gggccaaatt gtgctaccct gagcgattgc cacggtgatt
    16021 gtccaaggaa ttcccgccat gcaaaccgga aacattttca tcgtcaccgc gccgtccggc
    16081 gcgggcaaga ccacgctggt ggccgccctg ctggccgccg acaaggacgt gcagctgtcg
    16141 gtgtcgcaca ccacgcgcca accgcgcgcg ggcgaggtgc acggtgagca ctatcatttt
    16201 gttgaccgcg ccaccttcga ggcgatgatc gcccgcaagg attttctgga atacgccgag
    16261 gtgtatggca attactacgg cacctcgctg cgctggctgc gcgagcagct gtcgctgggg
    16321 cgcgacatcc tgctggaaat cgactggcaa ggcgcccagc aagtgcgcaa ggcgtttccg
    16381 caggcggtcg gcatttttat tttgccgccg tcggtcgaag ccttgggcga gcgcctgcgc
    16441 ggccgcgcca aggacagcga agccaccatc gaggcgcgtc tggccgtggt gcgctcggaa
    16501 atcgaccacg ccgaagaagc cgattatctg atcatcaatg aacagctgga tgacgccgtg
    16561 cgcgacctga tcgccgtagt gcgcgcccag cgcctgcgca ctgcccgcca gatgcaggcg
    16621 cgcggcgaac aggttcgcgc catgcaaagc caggcgcgtt gattctggct gttcttgtct
    16681 ctttcacctt agttttacgc actggaaaaa cacacccatg gcccgcatta ccattgacga
    16741 ctgcctgaaa cgcatcccca accgcttcga cctgaccctg gccgccacct atcgcgcccg
    16801 ccaactggcc tctggcgcca ccccgctggt ggaagccggc aaggacaagc ccaccgtgct
    16861 ggcgctgcgt gaagtggcca atggccacgt cggccgggaa atgctcgatc gccatcgcgg
    16921 ctgagactga cggcgcaacg ggcgaaagag cgagacgcgc atgagcagtg acgtgaacga
    16981 acggatcgac tacgacgcac tggtggccaa cgaggccgcc cagttgctga gcgccgcgcg
    17041 cgcctatgtg aagccggatg acctgccgct gatccggcag gcgtttgact tcagccgcga
    17101 cgcccacgaa ggccaggtgc gcaagagcgg cgagccgtac atcacccacc cgctggcggt
    17161 ggcgcacatc ctcaccgact ggaagatgga cgcccacggc ctggccgccg ccctgttgca
    17221 cgacaccatg gaagacaccg gcgtggccaa atccacgctg gaagagcgct ttggctcggt
    17281 ggtggccgag ttggtggatg gcctgtccaa gctggagcgg ctggaatacc agaccaaaga
    17341 aaccgcccag gcggagaatt tccgcaagat ggtgctggcg atggcgaaag acatccgcgt
    17401 catcatcgtc aagctggccg accgcctgca caatatgcgc accctggacg ccatgcgcga
    17461 ggacaagcgc cggcgcatcg cccaggaaac catcgacatc tacgcgccgg tggccaaccg
    17521 catcggcctg aacaaggtct accgcgaact gcaagacctg tgcttcaagc acctgcaccc
    17581 caaccgctac caagtgctgc acaaggccat ccgcgccgca cgcggcaacc gccgcgagct
    17641 ggtcagcaag atcatgcagg cgctatcgca aaaactggtg caggccaata tcgaggcctc
    17701 gatcaagggc cgcgaaaaaa acctgtacag catctatcac aagatgcagg aaaaacagct
    17761 gtcgttttct gaagtgctgg acatctacgg ctttcgcgtc atcgtcaacg acattcccag
    17821 ctgttatctc gccctgggcg ccatgcacgc cttgtacaag cccattcccg gcaagttcaa
    17881 ggattacatc gccattccca agagcaatgg ctaccaaagc ctgcacacca cgctgtttgg
    17941 cccctatggc acgccgatcg aggtgcagat tcgcacgctg gacatgcacc atatcgccga
    18001 agccggcgtg gccagccact ggatgtacaa gagcggcgac gaatccatcg acaaggccca
    18061 gcagcgcacc catcagtggc tgcaagaaat cctggagctg caagccggct caggcgacgc
    18121 catcgagttt ctcgaacacg tcaaggtgga tctcttcccc gacgaagtct atgtgttcac
    18181 ccccaagggc cgcatcctgg tgctgccgca aggctccacc ccggtggatt ttgcctacgc
    18241 cgtgcacacc gatatcggcc accgctgcat tgccgccaag atcaatcacg agctggcgcc
    18301 cttgcgcacg ccgctgaaaa atggcgacca ggtggaagtg atcaccacca cccagtccaa
    18361 gcccaacccg gcctggctgt cgtttgtcgc cagcggccgc gcgcgcagcc acatccgcaa
    18421 ctacctgaaa agcctggagc gcgacgaagc cgcccacctg ggcgagcgtc tgctcaaaca
    18481 agccgtgcag gcgctggcca cccgcagttt gtcggtggac gccgatacct ggcaggcgta
    18541 tttgcgcgaa ttcgccgaaa aaggccagaa gtccagcgat gtgctgatgg aaatcggcat
    18601 cggcaagcaa ttgccgatgg tggtggccag ccgcctgctg gagctggctg gcgaagcgct
    18661 gggcgaagac gtgcgcgccg gcccggtgat gattcgcggc aacgaaggcg ccgccgtgca
    18721 gtttgccgcc tgctgcaacc cgattccggg cgaccgcatc gccggcgtga tcaacaaggg
    18781 ccagggcctg atgattcacg cccacgactg cccgacgctg gggcgcatcg accacgaccg
    18841 ccagctggaa gtcgaatggg acctggtcga accacgtctg tttggcgtgc caatcaccgt
    18901 gctggcgtac agcgaacgcg gcgcactggc ggcggtggcg gccgccatca ccgaagcgca
    18961 tgccaatatc gaaagcgtgg acatgcagaa cgcccacacc ggtgaaggcg ccatccagat
    19021 tcgcttccgc ctgcaagtgg aaaacatcct gcatctggcc gatgtgctgg ccaatatcca
    19081 ggaacagccc accgtggtgc gcgccgtgcg cggtatcctc agcgaaccgg ccgccagcaa
    19141 ggggcactga ccacacccgc ccccctcctg gcgctttacc gcctgccgcc gcgcgcctgg
    19201 ccaggcgcgt gcgcgcgttc tatgctttct gggtgatgga tgaccggagc gcacaatgga
    19261 cgccgacgat tacgacaacc cctggaagca ggcgattgat gagtatttcg ccgatttcat
    19321 cgcctgctat tttccgcaag cccaccaaga catcgactgg gcggccggtt atgaaaccct
    19381 ggacaaggag ctgcccggcc tggcgcgcga cgccgacctg gggcagcgtt acgccgataa
    19441 attggtgaaa gtgcgcaccc gccagggtca gcatgatctg gtctacgtgc atatcgaagt
    19501 gcagggtcag cacgaccctg actttgccca gcgcatgttt gtctatcact accggatttt
    19561 tgaccgcttt ggccagccgc cggtctctct ggcggtgttg gccgatgagc atccgcactg
    19621 gaagccaagc cgctatggct atcagcgttg gggatgtgcg ctggagtttg cgttccccac
    19681 cgtgaaattg agcgaacaca cccaatcttt ggatgccctg ctggccagcg acaacccctt
    19741 tgccattctg accgctgccc actggctgac tcgccgcagc aagcgcaata tggacgagcg
    19801 cctggccagc aagttgcgct taatcaagct gttgtatcat aaaggctgga cccgtcaggc
    19861 cgtgctggat ttttttgctg tcatcgactg gatgatgcac ctgcccgagg cgctgaccca
    19921 gcaatttcgc cagcaattgg ctgattatga acaggagaac atcatgcgtt atgtgaccag
    19981 cgtggagcga caagcccgtg aggaaggtgt gctgactggg ctgcagcaag gaatgcaaca
    20041 agggatacag caaggtgagg cggctttttt gctgcggctg ctgaatcgcc gttttggcgc
    20101 cttgccggcc gagtgggtgc tgcgcgtgca gcaggcaagt cacgccgagc tcgaactgtg
    20161 gggcgagcgg gtgctggatg cggcttcgct ggacgaggtg tttgccccgc agacgcacta
    20221 agagcggcta acaaaaccct cctggcgttg ttgcccgacc ttgccgtact acc
//
LOCUS       DAOPYX010000017        19927 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_2821_length_19927_cov_8.259813, whole genome shotgun sequence.
ACCESSION   DAOPYX010000017 DAOPYX010000000
VERSION     DAOPYX010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 19927)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19927)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19927
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2821_length_19927_cov_8.259813"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..724)
                     /locus_tag="PKN55_01805"
     CDS             complement(<1..724)
                     /locus_tag="PKN55_01805"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003790975.1"
                     /note="catalyzes the conversion of citrate to isocitrate
                     and the conversion of 2-methylaconitate to
                     2-methylisocitrate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase B"
                     /protein_id="HNX70009.1"
                     /translation="MLEAYRQHVAERAEIGIPPLPLNAKQTAELVELLKTPPAGEEAV
                     LVDLLTNRVPAGVDDAAKVKASFLAAVARGEAASPLVSREKATELLGTMLGGFNIKPL
                     IDLLGDAALGGIAADALKKTLLVFDFFHDVKELADKGNANAKAVLQSWADAEWFTSRP
                     EVPAVQKITVFKVTGETNTDDLSPAPDAWSRPDIPLHAMAMLKNPRDGITPEQAGVRG
                     PVKFLEDLRAKGNLVAYVGDVVG"
     gene            complement(932..1117)
                     /locus_tag="PKN55_01810"
     CDS             complement(932..1117)
                     /locus_tag="PKN55_01810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX70010.1"
                     /translation="MRTKQAPAWCGLGRKPLKARQKPARRIRARQTQFFVTFLPSAGK
                     NRRPWKKTGKSVAFSSH"
     gene            complement(1184..1828)
                     /locus_tag="PKN55_01815"
     CDS             complement(1184..1828)
                     /locus_tag="PKN55_01815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014087665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNX70011.1"
                     /translation="MDSATKDTATRILDAAERLFVEHGFEATSLRTITQQAEVNLAAV
                     NYHFGSKDALFQAVLVRRLAPLNQQCVAELDVLEKSGAPITVEGVLMTFIRPCLALSK
                     DPNRGGAMFVRLLSRTFVENHRLIRDTLPQQYSEFVNRYSKAFSRVLPGLKLEELAWR
                     LHLAFGLMFNAFAGNDVLKIFVKSDIVSARDPDTVVRHMIPFVIAGLTNPATQS"
     gene            2288..4723
                     /locus_tag="PKN55_01820"
     CDS             2288..4723
                     /locus_tag="PKN55_01820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009117266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HNX70012.1"
                     /translation="MSQTCFHCGQPVPPGVDFPIQDRDTQHAACCAGCQAVAQTILDS
                     GLGDYYRHRTAEARQADPLPDELLARLRLYDTPALQASFVRDEGGDVREAALMLEGIT
                     CAACVWLNERHLRGLPGVLSVDINYTSHRARVRWDNSRIALSAILESIAAIGYQAHPY
                     DAERQEKLAQQERKQAINRLWVAGLSMMQVMMYAVPIYLAPDGEIDPQWLWLLHWASF
                     ILTLPVVVYSAVPFYRATWRDLKSRRVGMDTPVTIGILTAFFASLWALLNHIDHGVYF
                     DSVSMFVFLLLGGRYLEGIARRKAGAAAESLVKLTPAFCHRLPDWPASQDSEEAVAAL
                     LNVGDVVRVHAGESFPADGEVLAGHSSVNESLLTGESRPLPRAPGGVVIAGSINLDSP
                     LTVRVSQVGPNTRLAGVVRLLDQALAEKPRLAKLADRFAGWFVAVLLLAAAASYLGWL
                     AIAPDRALWIMVAVLVVSCPCALSLATPAALTAATGHLAGIGMLTTRGHALETLAKVS
                     DAVFDKTGTLTHGDMRLLQTLPLGALPEAEALALARSMEAHAHHPIARAFAAAEHASA
                     PAPALDEVSYQAGCGLQAQVAGHTWRLGKPDYVAALAGPLPAALAGWRDDATVVALGS
                     AHGWAAAFAIGDALRDDAGALIASLRRHGVRCHILSGDAPGAVAAVAAKLGVDSAEGG
                     LLPEDKLARVAAWQAAGRRVLMLGDGVNDAPVLARADVSVAMGGGADVARMSGDMVLV
                     NDQLQRLDDAVLLARRTLSVIRQNLWWAAGYNLVALPLAISGHLTPWLASLGMACSSL
                     LVVSNALRLVRRG"
     gene            4784..5191
                     /locus_tag="PKN55_01825"
     CDS             4784..5191
                     /locus_tag="PKN55_01825"
                     /inference="COORDINATES: protein motif:HMM:NF021158.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaY family lipoprotein"
                     /protein_id="HNX70013.1"
                     /translation="MKRMFNPLAAAALALTLSACASWWGPSGPTLLQGQVRLNGNPPA
                     EVVAVEVRLIDRGEPRMPVTLAEQTLVHPRSLPVTFSLPVIDARRLPANGVYVLEARL
                     YDSQGERARTQEDVRWRPGDQQAVELLLTAKPR"
     gene            5686..6450
                     /locus_tag="PKN55_01830"
     CDS             5686..6450
                     /locus_tag="PKN55_01830"
                     /inference="COORDINATES: protein motif:HMM:NF024376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HNX70014.1"
                     /translation="MPFRVGVAPHTSARLILQQYQPLRASLEKGLGQPVEIATAPDFT
                     DFARRALRGEYDLAITTGHQALLLRDDAHYLPLVTYRAPFQAVVVGLASMPAGSSAAQ
                     LNGKTVLGLSPSSLVTLWGIHWLEQNKVKPQRMQFISAADSMAQLVIQHDAVVGFMSQ
                     ANYEKLSAETRAQLRVVARSPEMAGRIYLLHPRLASRQAQLMRLIQQFGQSAEGMQYF
                     QENKLDGYRLLKPGELEVMRPYADEVRRTLATQPGH"
     gene            6459..9272
                     /locus_tag="PKN55_01835"
     CDS             6459..9272
                     /locus_tag="PKN55_01835"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNX70015.1"
                     /translation="MHLVKTVRGRLLLAALLVEAVMLALLIGNSVRLLYDSLSDQAKR
                     HAEQMSPILNAALMAPLAQRDYATVRAILDESRGKNGILYLAVSDSRGVTVARSGWPA
                     GQPLPEPDASFTLDDKDEGGSRYDVDTPITLYGQKLGSLRFGLDLSHIVAARESLISQ
                     GVLIALGEIMLSAGLLTFLGLYLTRQLSELTRASEALARGQLASRLVAEGDDEIGRLG
                     RAFNAMSRAIRDRLDALHASEQRLNLALDGGELGLWDTDFRTGKITINPRCAAMLGET
                     HGQFHPDVKDWEARVHPEDRHHMRYAAVEHLKGHTAMFESEFRIRHHDGHWVWLLSRG
                     KVVERNSQSRAVRLIGTLLDINERKSTEARLRLLASVFRYAQEGIMITDADGRILEVN
                     RAYHDITGYSRAAVVGQPSDLLHEEGGADEAIRQTMRHALHSHGHWSGEIVNRRANGE
                     LFHEHLSLTSVHDAGGQIVNYIGIFTDISQIKAQQEKLERLARYDMLTGLPNRTLLAD
                     RLSTAIALTRRTGERTGVALIDLDGFKTINDTYGHETGDLVLKEVAQRLTRHIRDTDT
                     AARLGGDEFVLVLTDLRPGDEAHVLDKILAVLAEPYPLEAFGSVAHLSASIGLTLYPD
                     DTADADALLRHADQAMYSAKQQGRNRWHAFDPDIDRHVRARFESRDRVSEALQNRELV
                     LYYQPKVDLALGKVTGMEALLRWQHPERGLVTPGEFLPLIEGSEFEVELSRWVIRNVV
                     EQLHCWNQQGLTFHISANVPGQHLQSPDFVQQLENLFKLYPDVPRERLELEILETVAL
                     GDMEGVSRQMDDCRTLGVRFALDDFGTGYASLEYLRRLPVDVIKIDQTFVRDMLDDVN
                     DLAIVEGIIALSRAFRRQVVAEGVESQAHYLRLQELGCAVGQGYTIARPMPAAQVPTW
                     LANWQPQTLNTQPAPSGVAELNT"
     gene            9286..9507
                     /locus_tag="PKN55_01840"
     CDS             9286..9507
                     /locus_tag="PKN55_01840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018746342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SlyX family protein"
                     /protein_id="HNX70016.1"
                     /translation="MHDTEDRITELEIRLAWQDDLLDNLNQALIRQQQQLDLMQQQLR
                     RLYDMTTQQGQVESGSLLAQLQQEIPPHY"
     gene            complement(9628..9888)
                     /locus_tag="PKN55_01845"
     CDS             complement(9628..9888)
                     /locus_tag="PKN55_01845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000062 - fatty-acyl-CoA binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA-binding protein"
                     /protein_id="HNX70017.1"
                     /translation="MSDLQARFEQAQIDLKSLSERPDNAVLLELYALYKQATAGDVSG
                     ERPGMMDFINRAKYDAWERYRGYSSEAAMERYLEVANAQLGR"
     gene            10279..10458
                     /locus_tag="PKN55_01850"
     CDS             10279..10458
                     /locus_tag="PKN55_01850"
                     /inference="COORDINATES: protein motif:HMM:NF025436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjfB family protein"
                     /protein_id="HNX70018.1"
                     /translation="MSLTSNVSNAPTDVHLSASIFAAKKSQDLQQQNVATLLNSVATP
                     PSNPAHLGNKIDVRA"
     gene            11585..11830
                     /locus_tag="PKN55_01855"
     CDS             11585..11830
                     /locus_tag="PKN55_01855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing protein"
                     /protein_id="HNX70019.1"
                     /translation="MHSFNAKAGDTVRVELNGKNPLAYLTLIQPDGKEMVPAETRGLL
                     LTLPMDGQYSVKVFLFRDGAGSDGKPVDYRLRVLPVK"
     gene            complement(12127..13374)
                     /locus_tag="PKN55_01860"
     CDS             complement(12127..13374)
                     /locus_tag="PKN55_01860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008953305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-xanthine permease family protein"
                     /protein_id="HNX70020.1"
                     /translation="MFQQLKTVVSGAQILFVAFGALVLVPLLTGLNPAMALLGAGVGT
                     LLFQLCTGRQVPIFLGSSFAFIAPIIYATQTWGLPGTMFGLFAAGFMYFVFAALVRWR
                     GIEAVHRLLPPVVIGPVIMIIGLSVATVASNMAMGRAGGNQVMDYQQSLLLAAVSLSA
                     TVLVAVFARGMFRLVPILAGVIAGYVLAAMMGLVDFAKIANAPWFAVPHFITPEVNWA
                     AALFMLPVAIAPAIEHIGGVMAIGGVTGKDYTKTPGLHRTLAGDGLGVCVAGLIGGPP
                     VTTYAEVTGAVMITRNFNPVVMTWAALFAIAMAFFGKFNALLQSIPMPVMGGIMVLLF
                     GTIASIGLKTLIEAKVDLMAPRNLVIVSVVLTAGIGSLELKFGSFSLVGVGLCSILAI
                     VLNLILPRAERGPHDGVVEGQDV"
     gene            complement(13422..14057)
                     /locus_tag="PKN55_01865"
     CDS             complement(13422..14057)
                     /locus_tag="PKN55_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014086078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX70021.1"
                     /translation="MDAFQQIDQDFLLRFPGGLNDPEWLALAKKYRVVDKVRELAASL
                     DAATLAQALDSGRTTAVVDTLDKLISRCPTVSTFERMAFRHYLAYSDTHLPLIAALDG
                     VWRRCEAETFEQLVSVLEMCRHDKCANPAKWPVVTAFLAYADPDAHVHIKPTTTRQLA
                     ARLGVDIGYQSRPNFATYQRVREMVSAFRASSAVAHGQNYITTQAVMYCAL"
     gene            complement(14141..14770)
                     /gene="upp"
                     /locus_tag="PKN55_01870"
     CDS             complement(14141..14770)
                     /gene="upp"
                     /locus_tag="PKN55_01870"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014086079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HNX70022.1"
                     /translation="MPITIVNHPLVQHKLGLLREADISTYKFRTLTHELARLLAYEAT
                     RDFELEPVTIDGWCGDINVQRIKGKKVTVVPILRAGLGMLDGVLDLVPSAKISVVGLY
                     RNEETLEPVPYFDKFVDSLDERIALIIDPMLATGGSMIATIDLLKRKGCQSIKAIVMV
                     AAPEGVKAVSDAHPDVEIVTAALDSHLNEHGYIIPGLGDAGDKIFGTKG"
     gene            complement(14980..15870)
                     /locus_tag="PKN55_01875"
     CDS             complement(14980..15870)
                     /locus_tag="PKN55_01875"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNX70023.1"
                     /translation="MPDWLWPLLALAAAPAAGRALRAALLNRLRRSLTPERVRLPCAL
                     PPAQGILQPCRIATVGGLSLAGWHSPPGRPGAPVLLFVHGWGGSAAQLLPLIAPLHAA
                     GYGVLAWDARCHGHSDDDSYSALPRFAEDIEAALDWLAGQDWAHGAPRWLIGHSVGAA
                     ASLWTARRRAGDVAGVVSLSAFAHPGELMRRWLASKRIPYWPLGRWLLAQIQHIIGHR
                     FDDIAPLAHMPHLRCPVLLIHGERDDTIPSDDARRLQAAACHAPTQLLCLPGDHERAD
                     DLPGCVAQIQHFIAAHPAAR"
     gene            complement(15884..17296)
                     /gene="hemN"
                     /locus_tag="PKN55_01880"
     CDS             complement(15884..17296)
                     /gene="hemN"
                     /locus_tag="PKN55_01880"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017510076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HNX70024.1"
                     /translation="MTATNITLAPTRCEFDRVLIENLDGYGPRYTSYPTADRFHDGFT
                     ERDYRHWLQQRQIGASAWPLSLYVHLPFCNTVCYYCACNKVITKDRSKADTYLDYVEK
                     ELALHAQTMGGGVREVAQLHFGGGTPTFLNDAQLTRLMAAIRQHFHLVTGGEFSIEID
                     PRKVTADTVRLLAELGFNRMSVGVQDFEPEVQQAVNRIQSEEETRLVIDTARACGFKS
                     VSIDLIYGLPLQTEASVMRTVERVLAMDPDRLAIYNYAHLPHAFMPQRRINEAQLPSA
                     KVKLDILQAVVGRLTDAGYVFIGMDHFAKPDDDLSVALKQGRLQRNFQGYSTHADHDL
                     IGIGVSAIGKVGPTYSQNVRTLDEYYAALDADRLPTLRGMVLTEDDILRRNIIQRLMC
                     QFALSKEAIEEVHAIDFAGYFAPELEKLAEYEAHGMLTMDKEWITVTPKGRFLIRTIA
                     MVFDRHLREKQAQARYSRVI"
     gene            17393..18151
                     /gene="fnr"
                     /locus_tag="PKN55_01885"
     CDS             17393..18151
                     /gene="fnr"
                     /locus_tag="PKN55_01885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008953367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HNX70025.1"
                     /translation="MVEHTNIPIHTLKVSCSNCSLRELCLPVGLSREEMSQLDAVIRQ
                     SRRLKRGEYLFRAGEPFKSVFAVRTGFFKTCVGSHDGREQVTGFHMSGELLGLDGISA
                     NAHGCDSIALEDSEVCELPFARMESLGRDIPSLQHHFFRLMSREIVRDQSVMLLLGNM
                     KAEERLAAFLLNLSLRFSTRGFAANDFILRMSREEIGSFLGLKLETVSRTLSRFQANG
                     WITVEHKHIQLVQPTALKELISGCSGNTQQTHAM"
     gene            18299..19273
                     /locus_tag="PKN55_01890"
     CDS             18299..19273
                     /locus_tag="PKN55_01890"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNX70026.1"
                     /translation="MQYTDIAVIGAGLAGVSCARQLAAAGRQVTVFDKSKGMGGRMAT
                     RRGDGFSVDHGAQYFTAQQPDFHAEIHAWQTAGLCAPWQARLVQHRPTGPVDGYPEHR
                     YVATPGMSSLCRALAAALPVQLNHRLTALQRQADGWQLSFADQPSWRARQVLLAAPAP
                     QATALLDAAPALRALAAGVEMEACWAVMLHAQAPLEIGFDAAWVEHGPLRWLARNSSK
                     PGRSSAESWLLHATPAWSAAHIDAPADEVAGLLHAAFAELVDSAVDARNRSAHRWLYA
                     QAAQPLDIGSAYQAELGLGLCGDWCLGTRVEDAWRSGRHLAEQALAAA"
     gene            complement(19364..>19927)
                     /locus_tag="PKN55_01895"
     CDS             complement(19364..>19927)
                     /locus_tag="PKN55_01895"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNX70027.1"
                     /translation="AILADLPSLIEGLMEGAERSAAIVDGLKRFSATDRGGAERFDLA
                     DAVRRSAHWVEKAAPAHVQLLLDAPDAPVMLCGAAGRIQQVLINLVQNAVDATAHHPA
                     PRVSVRLTEADGWATVTVADNGPGLSPEVQQRLFEPFFTTKPVGEGTGLGLSISFGIV
                     EQHGGQLRADNAPEGGARFTLRLPLAN"
BASE COUNT         3812 a         6247 c         6245 g         3623 t
ORIGIN      
        1 tgccgaccac gtcgccgacg taggccacca ggttgccctt ggcgcgcagg tcttccagga
       61 atttcaccgg gccgcgcacg ccggcttgtt ccggggtgat gccatcgcgt gggtttttca
      121 gcatggccat ggcgtgcagc gggatgtccg ggcgggacca ggcgtccggg gccggggaca
      181 gatcgtcggt gttggtttcg ccagtgacct tgaacacggt gattttttgc acggccggga
      241 cttccggacg gctggtgaac cactcggcgt cagcccagga ttgcagcacg gccttggcat
      301 tggcgttgcc cttgtcggcc agttccttca cgtcgtggaa gaaatcgaac accagcaggg
      361 tttttttcag ggcatcagcg gcaatgccgc ccagcgcggc gtcgcccagg aggtcgatca
      421 gcggcttgat gttgaagccg cccagcatgg tgcccagcag ttcggtggcc ttctcgcggg
      481 aaaccagcgg ggaagcagct tcgccacggg cgacagcggc caggaacgag gccttgacct
      541 tggcggcgtc atccacacca gccggcacgc ggttggtcag caggtccacc agcacggctt
      601 cttcgccagc cggcggggtt ttcagcagct cgaccagttc ggcggtttgc ttggcgttca
      661 ggggcagcgg ggggattccg atttcggcac gttctgcgac atgctgacga taagcttcta
      721 gcacagcttc atcctctcta tttcaggcaa gagtatctgg tgggggtaaa acgtcccggc
      781 gggtcggcaa acccggaaca ccggctcgaa agcggaagct gtacaggcga gggcgacatg
      841 cggcgcaagg ctgcggggcc accggatgcc tggagcgggt ccgtgcggga taaggcctgt
      901 cgaaacggcc caattttatc ggaaagcgga cttaatgtga agaaaatgcg acacttttcc
      961 cggttttttt ccacggacgg cgatttttgc cggcagatgg caggaatgtg acaaaaaact
     1021 gggtttgccg cgcgcggatg cggcgggcgg gtttttggcg tgctttcagc ggtttacgcc
     1081 caagcccgca ccaagcgggc gcttgcttgg tgcgcatggc gcacgcacgc cagcgccgga
     1141 tggctcaggc atgcgggttt gcccgaattg cgccaggtgc ggctcaggat tgggtggcgg
     1201 gattggtcag gccggcaatc acgaacggaa tcatgtggcg caccacggtg tccgggtcgc
     1261 gggcagagac aatatcgctc ttgacgaaga ttttcagcac gtcgttgccg gcaaaggcgt
     1321 tgaacatcag gccaaaggcc agatgcaggc gccaggccag ttcttccagc ttcaggccgg
     1381 gcagcacgcg ggaaaacgcc ttgctgtagc gattgacgaa ttcgctgtat tgctgcggca
     1441 gggtgtcgcg gatcagccgg tggttttcca caaaggtgcg cgacagcagg cgcacaaaca
     1501 tggcgccgcc gcgattgggg tctttggaca gcgccagaca ggggcggatg aaggtcatca
     1561 gcacgccttc gacggtgatt ggcgcgccgg atttttccag cacgtccagc tcggccacgc
     1621 attgttgatt caggggcgcc aggcgacgca ccagcacggc ctgaaacagc gcgtctttcg
     1681 agccaaagtg atagttgacc gcagccaggt tcacttcggc ttgctgggtg atggtgcgca
     1741 gcgaggtggc ctcaaagccg tgttcgacga acagacgctc ggccgcgtca agaatgcggg
     1801 tggcggtgtc tttggtggcg ctatccatgg ttttccgttg ggggggatta agagtctgag
     1861 tctctgtcat tatattttta taagtggttg tttgaaactt ttgttcatgc cgaatggcgc
     1921 acaagccggc atcccagagc ttgaccgccg gattcggggg gcgatgctca gcggtcaatg
     1981 ttgtgctttg tatcatgatc cgctgcattt gcccatgggg acatcatact ttacgaacaa
     2041 gcacttaggc ttttttctgc cagtttgatg gctgtttgtc agcggcttgt gcgcacaaag
     2101 accatgccat gagcgatttg tgctgaaaag ccacagaatt ccgacaggcg gaacgccgct
     2161 ggccacatgg cgggcagata tttgctggcg tgcacaaatt gcatcaatta cccgtccggg
     2221 ctgaggcgcg gggtttttcc acacggcgct tggcggcagg ctggcccggt atgtcacaat
     2281 accggccatg agccagacat gttttcactg cggccagccg gtgccgccgg gggtggattt
     2341 ccccattcaa gaccgcgaca cccagcacgc cgcctgctgc gccggctgcc aggcggtggc
     2401 gcaaaccatt ctcgacagcg gcctgggcga ttattaccgc caccgcaccg ccgaggcccg
     2461 ccaggccgac ccgctgcccg atgagctgct ggcgcggctg cggctgtacg acaccccggc
     2521 cttgcaagcc agttttgtcc gcgacgaggg tggcgacgtg cgcgaggcgg cgctgatgct
     2581 ggagggcatc acctgcgcgg cctgcgtgtg gctgaacgag cgccatttgc gcggcctgcc
     2641 cggcgtgctg tcggtggaca tcaactacac cagccaccgc gcgcgggtgc gttgggacaa
     2701 ttcgcgcatc gccctgtcgg ccattctgga atccatcgcc gccattggct atcaagccca
     2761 cccttacgac gccgagcgcc aggaaaaact ggcccagcaa gagcgcaagc aggcgattaa
     2821 ccggctgtgg gtggccgggc tgtcgatgat gcaggtgatg atgtacgccg tgccgattta
     2881 tctggcgccg gatggcgaaa tcgacccgca atggctgtgg ttattgcact gggccagttt
     2941 tatcctcacc ctgccggtgg tggtgtattc ggccgtgccg ttttatcgcg ccacctggcg
     3001 cgacctgaaa tcgcggcggg tgggcatgga tacgccagtc accattggca ttctcaccgc
     3061 gttttttgcc agcctgtggg cgctgctcaa tcacatcgac catggcgtgt attttgattc
     3121 ggtgtcgatg tttgtctttt tgctgctggg cgggcgctat ctggaaggca ttgcccggcg
     3181 caaggccggc gccgccgccg aaagcctggt caagctcacc ccggcgtttt gtcaccgtct
     3241 gcccgactgg ccagccagcc aggacagcga agaagcggtg gccgccctgt tgaatgtggg
     3301 cgatgtggtg cgtgtgcacg ctggcgagag tttcccggcc gatggcgagg tgctggccgg
     3361 gcacagctcg gtgaacgaat cactgctgac cggcgaaagc cggccgctgc cgcgcgcgcc
     3421 gggcggcgtg gtgattgccg gcagcatcaa cctggattcg ccgctgaccg tacgggtgag
     3481 tcaggtgggg ccaaacaccc ggctggccgg tgtggtgcgc ttgctggatc aggcgctggc
     3541 ggaaaaaccc cgcctggcca agctggccga ccgctttgcc ggctggtttg tcgccgtgct
     3601 gctgttggcc gccgccgcca gttatctggg ctggctggcc attgcgccag accgtgcgct
     3661 gtggatcatg gtggcggtgc tggtggtgtc ctgcccgtgc gcgctgtcgc tggccacccc
     3721 agcggcgctg accgccgcca ccggccatct ggccggcatt ggcatgctga ctacgcgcgg
     3781 ccatgcgctg gaaaccctgg ccaaggtcag cgacgcggtg ttcgataaaa ccggcacgct
     3841 tacccacggc gatatgcgcc tgttgcaaac cttgcccttg ggcgcgctgc ccgaggccga
     3901 ggcgctggcg ctggcgcgca gcatggaagc ccacgcccat cacccgattg cccgcgcctt
     3961 tgccgccgcc gaacacgcca gtgcgcccgc cccggcgctg gacgaagtca gctatcaggc
     4021 gggctgtggc ttgcaagcgc aagttgccgg ccacacctgg cggctgggca agccggacta
     4081 tgtggccgcg ctggccgggc ccttgcccgc cgcgctggcc ggctggcgcg acgacgccac
     4141 ggtggtggcg ctgggcagcg cgcacggctg ggcggcggcg ttcgccattg gcgacgcgct
     4201 gcgcgacgac gctggcgcgc tgattgccag cctgcgccgg catggcgtgc gctgccatat
     4261 tttgtctggc gacgcgccgg gcgcggtggc ggcggtggcg gccaagctgg gggtggacag
     4321 cgccgaaggc ggcctgctgc cagaagacaa actggcgcgg gtggcggcct ggcaggcggc
     4381 cggccgccgg gtgctgatgc tgggcgacgg ggtgaacgat gcgccagtgc tggcgcgcgc
     4441 cgatgtgtcg gtggccatgg gcggcggcgc cgatgtggcg cgcatgagcg gcgatatggt
     4501 gctggtcaac gatcagttgc agcggctgga cgacgccgtg ctgctggcgc ggcgcacgct
     4561 gagcgtgatt cgccagaatc tgtggtgggc ggcaggctat aacctggtgg cgctgccgct
     4621 ggccatcagc ggccacctca ccccctggct ggccagcctg ggcatggcgt gcagctcttt
     4681 gctggtggtc agcaatgcgt tgcggctggt gcggcgcggc tgaacctgtc gcccgcgcgc
     4741 cagtccaccg gataacttga ttcacctgaa caggatgcaa aacatgaagc gcatgttcaa
     4801 tcccctggcg gcagccgcat tggcgctgac cctgagcgcc tgcgccagct ggtggggccc
     4861 cagcgggcca acgctgctgc aaggccaggt gcggcttaat ggcaaccccc cggcagaggt
     4921 ggtggcggtg gaagtccgct tgatcgaccg tggtgaaccc cgcatgccgg tgacgctggc
     4981 ggaacagacg ctggtgcacc cgcgcagcct gccggtcact tttagcctgc cggtcatcga
     5041 cgcccgccgg ctgcccgcca acggcgtgta tgtgctggaa gcccggttat acgacagcca
     5101 gggcgagcgc gcccgcacgc aggaagacgt gcgctggcga ccaggcgatc agcaggctgt
     5161 tgaactgctg ctgacggcca agcctcgctg agcatcgctt acacaatcct gctggcgttg
     5221 tgacctgtat tacctgccgg cgcgtgtcca gccagaaacg ctgcgttgag ttttatgatc
     5281 gatgctaaaa gaatgtgtgg ctggtggttt gacggtgaaa ttaatgctgt aagcatattg
     5341 ctgtttcttg gtaatgtcgt ttgcaggatt tattgccatc atcaacccga caaaaccttg
     5401 acttgactgc ttgcgagcct gcataactac gataacaata aggcgtggac gtcccgattg
     5461 atccgctcaa tgacggcaag acgggagtcc tcgccgttca agcgggttgc accggcatac
     5521 gcgggtgaca atgcgcaaaa aagttgtcgc cgctgcgcaa ggtatgggcg cacggagaaa
     5581 cgatggagga gttcagcaat gcacacggtc tgtcatgggt gcatgcgctg gctatacgca
     5641 atggcatggc tgctgggtgc agcgtgtttt gcgatggcca gtgacatgcc gtttcgggtc
     5701 ggcgtcgcgc cccataccag cgcgcgcctg attttgcagc aataccagcc tttgcgcgcc
     5761 agcctggaaa aaggcctggg tcagccagtg gaaattgcca ccgcgccaga ctttactgat
     5821 tttgcccgcc gtgccctgcg cggcgaatat gatctggcca ttacgaccgg gcatcaggca
     5881 ctgctgctgc gtgacgatgc gcactatctt cctctggtca cttaccgggc gccttttcag
     5941 gccgtggtgg tggggctggc ctccatgcca gcgggcagct cggcagccca gctcaatggc
     6001 aaaaccgtac tgggcctgag cccttcctca ctggtcaccc tgtggggcat tcactggctg
     6061 gagcaaaaca aggtcaaacc gcagcgcatg caatttatca gtgcggccga cagcatggcg
     6121 cagctggtga ttcagcatga tgcggtagtc ggcttcatgt cgcaggccaa ctacgaaaaa
     6181 ctcagcgccg aaacccgcgc acagctgcgc gtggtggcgc gcagcccgga aatggccggg
     6241 cgcatttatt tgctgcaccc ccgtctggcc agccgacagg cacaactgat gcgattgatc
     6301 cagcagtttg gccaaagcgc agagggcatg cagtattttc aggaaaacaa gctggacggc
     6361 taccgtctgc tcaaaccggg tgagctggaa gtgatgcggc cttacgcaga cgaagtgcgt
     6421 cgcacgctgg ccacacagcc cggacattag gacatggcat gcatttggta aaaacagttc
     6481 gtggacgtct gctgctggcc gccttgctgg tggaggcggt catgctggcg ctgctgattg
     6541 gcaacagtgt acgcctgctg tacgacagcc tgtcagatca ggccaagcgt catgccgaac
     6601 aaatgtcgcc catcctgaat gccgcgctga tggccccgct ggcacagcgt gactacgcca
     6661 cggtgcgcgc cattctggac gaaagccgag gcaagaacgg tattctgtat ctggccgtca
     6721 gtgattcgcg cggcgtcacc gtggcccgca gtggctggcc tgcaggtcaa ccactgcctg
     6781 agcccgatgc gtccttcacc ctggatgaca aggacgaagg tggctcgcgc tatgatgtgg
     6841 atacccccat caccttgtat ggccaaaaac tgggcagcct gcgttttgga ctggatttga
     6901 gccatattgt ggccgcgcgg gaatcgctga tcagccaggg cgtgctgatt gccttgggcg
     6961 aaatcatgct ctctgccggt ctgctgacct ttctggggct gtatctcacc cgtcagctgt
     7021 ctgagctgac ccgtgccagc gaagctctgg cgcgcggcca gttggcgtca cgactggtgg
     7081 ctgaaggcga cgacgaaatc ggccggctcg gtcgcgcctt caacgccatg tcgcgggcca
     7141 tccgcgaccg gctggatgcg ctgcacgcca gtgagcaacg gctgaacctg gcgctggacg
     7201 gcggcgaact cggcctgtgg gataccgatt ttcgcaccgg caaaatcacc atcaaccctc
     7261 gctgcgccgc catgctggga gaaacccatg gccagtttca cccggatgta aaagactggg
     7321 aggcccgggt tcacccggag gaccgccacc atatgcgtta cgccgccgtc gaacacctga
     7381 aaggccacac cgcgatgttc gagagcgagt tccgcatccg tcatcatgat ggtcactggg
     7441 tatggctgct cagtcgtggc aaggtggtcg agcgcaacag ccagtcgcgc gccgtgcggc
     7501 tgatcggcac cctgctggac atcaacgaac gcaaatccac cgaagcgcgc ctgcgcctgt
     7561 tggccagtgt gttccgctat gcacaggaag gcatcatgat taccgatgcc gacggccgta
     7621 ttctggaagt caaccgtgcc taccatgaca tcaccggcta ttcgcgtgcg gcggtggtgg
     7681 gccagccatc tgacctgttg catgaggaag gcggcgctga cgaggccatc cgccagacca
     7741 tgcgccatgc cctgcacagc catggacact ggagcggcga aatcgtcaat cgccgggcca
     7801 acggtgaact gtttcacgaa cacctgagcc tgacctcggt acacgatgcc ggtggccaga
     7861 tcgtgaacta tatcggcatc tttaccgata tttcgcagat caaggcccag caggaaaaac
     7921 tcgaacgcct ggcccgctac gacatgctca ccggcctgcc caaccgcacc ctgctggccg
     7981 accggctgag cactgccatt gccctgaccc gacgcaccgg cgagcgcaca ggcgtggcac
     8041 tgattgatct ggatggtttc aaaaccatca acgacaccta tggtcacgaa accggcgatc
     8101 tggtgcttaa agaagtggcc cagcgcctga cccgccatat ccgcgacacc gacaccgcag
     8161 cccgactggg gggcgatgag ttcgtgctgg tgctcaccga cctgcgccct ggtgacgaag
     8221 cccacgtgct ggacaaaata ctcgctgtcc tggccgagcc ctatccgctg gaagcctttg
     8281 gctcggttgc ccatctgtca gccagtatcg gcctgacact gtatccggat gacaccgccg
     8341 atgctgatgc gctactgcgc catgccgacc aggccatgta ttccgccaag caacagggcc
     8401 gcaaccgctg gcatgcgttc gaccccgaca ttgaccgcca tgtgcgcgcc cgctttgaaa
     8461 gccgcgatcg ggtatccgaa gccctgcaaa accgggagct ggtgctctac taccaaccca
     8521 aagtcgatct ggctttgggc aaagtgacgg gcatggaagc cctgctgcgc tggcaacatc
     8581 ccgaacgtgg actggtcacg ccgggggaat tcctgccact gatcgaaggc agcgagttcg
     8641 aggtagagct atcacgctgg gtgattcgca atgtggtgga acaactgcac tgctggaacc
     8701 agcagggcct gactttccac atcagcgcca atgtgcctgg ccagcatttg caatcgccgg
     8761 attttgtcca gcaactggaa aatctgttca aactgtaccc ggacgtgccg cgcgaacggc
     8821 tggagctgga aattctggaa acggtggcct tgggagatat ggaaggcgtg tcgcggcaaa
     8881 tggacgactg ccgcaccttg ggcgtgcgct ttgccctgga tgactttggt accggctatg
     8941 cctcgctgga atacctgcgt cggctgccgg tggatgtgat caagatcgac cagacctttg
     9001 tgcgcgacat gctggatgat gtcaacgacc tggccattgt ggaaggcatt attgccctgt
     9061 cgcgtgcctt tcgtcgtcag gtggtggccg aaggggtaga atcgcaggcc cattacctgc
     9121 gtctgcagga actgggttgt gcggtggggc agggctacac catcgcccgg ccgatgccgg
     9181 ccgcacaagt gccaacctgg ctggccaact ggcagccaca aaccttgaac acccagccgg
     9241 ccccgtctgg cgtggccgaa ctgaacacct gagcccacac cctgcatgca cgacaccgaa
     9301 gaccgcatca ccgaactgga aatccgcctg gcctggcagg atgacctgct ggacaacctg
     9361 aaccaggcgc tgattcgcca gcaacagcag ctggatctga tgcaacaaca gctgcgccgg
     9421 ctgtatgaca tgaccaccca gcagggccag gtcgagtcag gcagcctgct ggcccagctt
     9481 cagcaggaaa tcccaccgca ttattgaacg ggatgcagat aggccatgac caggatcgcc
     9541 aacatggcaa gcgcttgcga caagcgggtt atgatgatcg tccgcccatg ccgcatggcg
     9601 cgtgccgcag gaacatccgc tgaaatatca acgacccagc tgtgcgttgg ccacttccag
     9661 ataacgctcc atcgccgctt cgctgctgta gccgcgatag cgctcccacg cgtcgtattt
     9721 ggcgcgattg ataaaatcca tcatgccagg acgctcgccg ctgacatcgc cagcggtagc
     9781 ttgcttgtac agggcgtaca actccagtag cacggcattg tccgggcgct cggacaggga
     9841 tttcagatca atctgggctt gctcgaaacg ggcttgcaaa tcgctcatgg cggtgtctcc
     9901 tgtaaattta gcaggcagtt gctgcgtgtc cggcactgta gcgccgccag atagcacagt
     9961 caaacacttg tttgattttt gctgcattca ggccatgcgt atctgtaagc acttttgatg
    10021 taaatcacaa ttacatgtaa ttttgtccat ttacgtattt ctacgattga acattttcgg
    10081 caaagcgcat tcaatagcaa agtgaagtgt gctctgatgt ggcacgccac acgaaaacac
    10141 ttcaccatca ggtgattgaa cgatggaaga agcgctcaca aggccgttgc cacagggttt
    10201 gccagccttg tgagcgcttc ttcggctttt attcttcagc accaggatat ttaaaacaaa
    10261 tatttcatgg agttcatcat gagcctcacc tccaacgtca gcaacgcccc gaccgatgtg
    10321 catctgtcgg ccagcatttt tgctgccaaa aagtcgcaag acctgcaaca acagaacgtg
    10381 gccaccctgc tgaactcggt ggccaccccg ccgagcaatc ctgcccacct gggcaacaag
    10441 atcgacgttc gcgcctgaac gtcgaactga cgctattcag cagccaggca tgaggcttgg
    10501 cctcaggcat caggaccgga cctgacgctc gccagacagg ctgcccatac gcgccaggaa
    10561 tgcacgcccg gatggaaccc catccgggcg ttttcacgac tgccgccagc ggctggcatc
    10621 aggtaaactg gacacgatac gggcaagctc tcagcctcgc tcaccagacc cagcaacacg
    10681 tctgggccag gcatgcacag caggacaaca gaccgatctg gtgaactacg cccgggcatg
    10741 aagcccgcac gcccatgata ctgaacataa ggagaatgtc ttgatgcgcc gaaccctctg
    10801 cctgttgacc tgggcagccg tcagtggcgg tgccctggcc caagaagcca tgccactgca
    10861 agtggtgcca ttgcaacgcc caggcaatgc tgcgcccgcc aaaccggccg acggcactgg
    10921 tgataccgcg tctgccgaca aggctgccag caagccggca gccaccaagt ctggcacatc
    10981 cggcaagcat gagacccaca aaccggccgc cgccaaacca gatgaccgcc cggctgacaa
    11041 acccgccagc gacaacacac aggtcaaaac cctggcgccc gaagtcaagc tttccagtcc
    11101 gccgcccagc cctgcggcca ccagcaaacc tacggccccg gcagaaaaac tggcccccaa
    11161 ggcgaacacc aacaacacac cggcaggcag accaccggtg gcctctcacg ctgccgacac
    11221 ccgccacaac tccgccacgt cagcagacaa acccgccagc aaacccgcag cacccgcggc
    11281 tgacaccagc aaacctgccg cggacaaacc ggtgagcaag ccggccagcc cacctgccgt
    11341 ggcgccgagc cgtgcagcca gcccggcgcc tgcgccggtc aagatggccg tcgccccacc
    11401 tcgaccggaa atgttcccca aagaaccggc cgctgagctt gtctctccga cccctgctcc
    11461 gcaagccaca ccgccggctg caccggccaa gccacgccca ccggcagcca agccagctgc
    11521 cgccaaaccg gccaaagccg cgggccagac atttaccgga accctcagtg gcgccaccag
    11581 caaattgcac agcttcaacg ccaaggccgg cgataccgtg cgcgtagaac tgaacggcaa
    11641 gaatccgctg gcatacctga ccctgattca gccggatggc aaagagatgg tgccggcaga
    11701 aacccgtggc ctgctgctta ccttgccgat ggatggccaa tacagcgtca aggtattcct
    11761 gttccgggat ggtgcaggca gcgacggcaa acccgtggac taccggctgc gcgtgctgcc
    11821 ggtcaaataa gcgctactca caacacgccg gggtgcgcgt gcggctttgt atgcggcttg
    11881 tactgtctgc ggccgcgtgc ctggccagaa ctgcttcgct gggtttgtga gcaacgctta
    11941 agcgcagctc ctggcatggt tgagtggtct tgtcgaacga ggcgtcaggc gcactgtgcg
    12001 aaatggggtg aaccacttgg ctgccgcttt gccattgagc tccaaataaa cgcctccaaa
    12061 tgacacaggg cggtgaagca agcttcaccg ccctgtgtcc attcatcagc gccgtccaat
    12121 cagcgctcag acatcctgac cttccaccac gccatcatgt gggccacgct cggcgcgcgg
    12181 caggatcagg ttcagcacaa tggccagaat cgagcacagg cccacgccca ccagcgagaa
    12241 gctgccgaac ttcagctcca gcgagccaat gccggcggtc agcaccaccg agacaatcac
    12301 cagattgcgc ggggccatca ggtccacctt ggcttcgatc agggttttca ggccaatgct
    12361 ggcgatggtg ccgaacagca gcaccatgat gccgcccatc accggcatcg ggatggattg
    12421 cagcagggcg ttgaacttgc caaaaaacgc catggcgatg gcgaacaacg ccgcccaggt
    12481 catcaccacc gggttgaagt tgcgggtgat catcaccgcg ccggtcactt cggcataggt
    12541 ggtcaccggc gggccgccaa tcaggccggc cacgcacacg cccaggccgt cgccggccag
    12601 ggtgcggtgc aggccggggg ttttggtgta gtccttgccg gtcaccccgc caatcgccat
    12661 caccccgccg atatgctcaa tcgcaggggc aatcgccacc ggcagcataa acagcgccgc
    12721 cgcccagttg acttccgggg tgataaagtg cggtacggca aaccagggcg cgttggcgat
    12781 cttggcaaag tccaccagcc ccatcatcgc cgccagcaca tagccggcaa tcactccggc
    12841 cagaatcggc accaggcgga acatgccgcg agcaaacact gccaccagca cggtggcgct
    12901 cagcgacacc gctgccagca acaacgattg ctggtagtcc atcacctgat tgccgcccgc
    12961 gcggcccatg gccatattgc tggccacggt ggccaccgac aggccgatga tcatgatcac
    13021 cgggccaatc accactggcg gcagcagacg atgcaccgcc tcgatgccgc gccagcgcac
    13081 cagcgcggca aacacaaaat acataaagcc ggcggcaaac aggccaaaca tggtgcccgg
    13141 caagccccag gtttgcgtgg cgtaaataat cggggcaata aaagcaaagc tggagccgag
    13201 aaaaatcggc acctggcggc ccgtgcacag ctggaacaat aacgtgccca cgccagcgcc
    13261 cagcagcgcc atggccgggt tcagcccggt cagcagcggc accagcacca gcgcgccaaa
    13321 ggcgacaaac aaaatctgcg ccccggacac gacggtcttg agttgttgaa acatgttgat
    13381 ccttaaatga aaatttcagt caatggcggc cggcctggcc gtcacagcgc acagtacatc
    13441 accgcctgag tggtgatgta gttctggcca tgcgccaccg cgctgctggc gcgaaatgcg
    13501 ctgaccatct cgcgcacccg ctggtaggtg gcgaagttgg gccgcgactg atagccaatg
    13561 tccaccccca gccgcgccgc cagctggcgg gtggtggtgg gtttgatgtg cacatgcgcg
    13621 tccgggtcgg cgtaggccaa aaacgccgtc accactggcc acttggccgg gttggcgcat
    13681 ttgtcgtggc gacacatctc cagcacgctc accaattgct cgaatgtctc cgcctcgcag
    13741 cgtcgccaca cgccgtccag cgcggcaatc agcggcaaat gggtgtcgct gtaggccaga
    13801 taatggcgaa acgccatgcg ctcgaaggtg ctcaccgtcg ggcagcggct gatcagtttg
    13861 tccagcgtgt ccaccaccgc cgtggtgcgg ccgctgtcca gcgcctgcgc cagcgtggcg
    13921 gcgtccaggc tggcggccag ctcgcgcacc ttgtccacca cgcggtattt tttggccagc
    13981 gccagccatt ccgggtcgtt caagccgcca ggaaaacgca acaaaaaatc ctggtcgatc
    14041 tgctgaaaag cgtccattcg tgcgttcctt gggtgaggcg gcaaacaaaa gcaagtgcgg
    14101 cagcgcagcg cgtcaggctc gccgacgcgc aaacgtggcg ttatcccttg gtgccaaaaa
    14161 tcttgtcgcc ggcatcgccc aggcccggaa tgatatagcc gtgctcgttc aggtggctgt
    14221 ccagcgcggc ggtgacgatt tccacatccg ggtgggcgtc gctgactgct ttcacccctt
    14281 ccggcgccgc caccatgacg atggccttga tgctctggca gcccttgcgc ttgagcaaat
    14341 cgatggtggc gatcatcgag ccgccggtgg ccagcatcgg gtcgataatc agggcaatgc
    14401 gctcgtccag gctatcgaca aatttgtcga aatacggcac cggctccagg gtttcttcgt
    14461 tgcggtacaa gcccaccacg ctgatcttgg ccgacggcac caaatccagc acgccgtcca
    14521 gcatgccaag gccagcgcgc aggatgggca ccacggtgac ttttttgccc ttgatgcgct
    14581 gcacattgat gtcaccgcac cagccgtcga tggtgaccgg ctccagctcg aagtcgcggg
    14641 tggcttcata cgccagcaga cgcgccagct cgtgggtgag ggtgcggaat ttatacgtgc
    14701 tgatgtccgc ctcgcgcagc aggccgagtt tgtgctgcac cagcggatgg ttgacgatgg
    14761 taataggcat ggttccccgc tcttgaatca aacgtccgac agcttgaaca gacgcaggcg
    14821 cggcggccag tgtggccggg cgccgatggt atgaaatact gtcactggcg cacgcggtcg
    14881 gcgccgccag gcaaaccggt taattttacg cgctcgggcc gggcaacgcc acgcgcaagg
    14941 ccggttttgc cgtgattgtg cgtgaattgg ccgatttact cagcgggcag cggggtgggc
    15001 ggcaataaaa tgctgaatct gcgccacaca gccgggcagg tcgtcggcgc gttcgtggtc
    15061 gccgggcagg caaagcagct gggttggggc gtggcaggcg gcggcctgca agcggcgggc
    15121 gtcgtctgat ggaatggtgt cgtcgcgctc gccatgaatc agcaacaccg gacagcgcag
    15181 gtgcggcata tgcgccagcg gggcaatgtc gtcaaagcga tggccaatga tgtgctgaat
    15241 ctgcgccagc agccagcggc ccaagggcca gtagggaatg cgtttgctgg ccagccagcg
    15301 gcgcatcagc tcaccggggt gggcaaacgc cgacaagctg accacgccag ccacatcgcc
    15361 tgcccgacgg cgcgccgtcc acaggctggc ggcggcgccg actgaatggc caatcagcca
    15421 gcgcggcgcg ccgtgcgccc agtcttgccc ggccagccaa tccagcgcgg cttcgatgtc
    15481 ttcggcaaag cgcggcagcg cgctatagct gtcgtcgtcg ctgtggccat ggcagcgggc
    15541 atcccaggcc agcacgccat agccggcggc gtgcagcggg gcaatcagcg gcagcaattg
    15601 cgcggcgctg ccgccccagc cgtgcacaaa cagcaagacc ggcgcgccgg gccggccagg
    15661 tgggctgtgc cagccggcca ggctcagccc gcccacggta gcgatgcggc aaggctgcaa
    15721 tatgccctgt gctggcggca gcgcgcacgg caggcgcacc cgctcgggcg tcaggctgcg
    15781 ccgcagccgg ttcagcagcg cggcgcgcaa ggcgcggccg gcggctggcg cagcggccag
    15841 cgccagcagg ggccacagcc aatcgggcat cagtcgggtg gagtcagatc acgcgggagt
    15901 aacgcgcctg cgcctgtttt tcgcgcaggt ggcggtcaaa caccatggcg atggtgcgga
    15961 tcaagaaacg ccccttgggg gtgacggtga tccattcttt gtccatggtc agcatgccgt
    16021 gggcttcgta ctcggccagt ttttccagct cgggcgcaaa gtagccggca aagtcgatgg
    16081 cgtgaacttc ttcgatggct tctttggaca gggcaaactg gcacatcagc cgctgaatga
    16141 tgttgcggcg caggatgtcg tcttcggtga gcaccatgcc gcgcaaggtg ggcagccggt
    16201 cggcgtccag cgcggcgtag tattcgtcca gcgtgcgcac attctggctg taggtcgggc
    16261 cgaccttgcc gatggccgac acgccaatgc cgatcagatc atggtcggcg tgggtggagt
    16321 agccctggaa gttgcgttgc agccggcctt gcttgagcgc cacggaaaga tcatcatccg
    16381 gcttggcaaa gtgatccatg ccgatgaaca cataaccggc gtcggtcagc cggcccacca
    16441 cggcttgcag gatgtccagc ttgaccttgg cgctgggcag ttgcgcttca ttgatgcgcc
    16501 gctgcggcat gaaggcatgt ggcagatgag cgtagttata aatggccaaa cggtctggat
    16561 ccatggccag cacacgctcg acggtgcgca tcacgctggc ttcggtttgc agcggcaggc
    16621 catagatcag atcaatgctg accgacttga agccacaggc gcgggcggta tcgatcacca
    16681 gccgggtttc ttcctcgctc tggatgcggt tgaccgcctg ttgcacttcc ggctcaaaat
    16741 cctgcacccc taccgacatg cggttaaagc ccagttcggc cagcaagcgc acggtgtcgg
    16801 cagtgacttt gcgcgggtcg atctcgatcg agaattcgcc gccagtcacc agatgaaagt
    16861 gctggcgaat cgctgccatc agccgggtga gctgggcgtc gttcaaaaac gtgggtgtgc
    16921 cgccgccaaa atgcagctgc gccacctcgc gcacaccgcc tcccatcgtc tgtgcgtgca
    16981 aagccagctc tttttcgaca taatccaggt atgtatctgc tttggagcga tccttggtga
    17041 tcaccttgtt acaagcgcag tagtagcaga cggtgttgca aaacggcaga tgcacataca
    17101 gcgacagcgg ccaggcgctt gcgccgatct gcctctgttg cagccaatgt cgataatccc
    17161 gctcggtaaa accgtcgtgg aaacgatctg ccgttggata cgacgtgtag cgcgggccat
    17221 agccgtcaag attttcaatc agtacgcgat cgaactcaca gcgagtcggc gctaacgtaa
    17281 tatttgttgc tgtcatcaca aaattccttg ggcaggttgc aaactttgca acaaacctgt
    17341 taagtatggt ttgacatgca tcaagctcgg gtcaccgttc atgcgaaaca ccatggttga
    17401 acacaccaat attccgattc acacccttaa ggtgtcctgc tcgaattgca gtttgcgcga
    17461 gctttgcctg cctgtgggcc tcagccgcga agaaatgtcg cagctggacg ccgtcatccg
    17521 gcagtcgcgc cggctcaagc gcggcgagta tttgttccgc gccggcgagc cgttcaagtc
    17581 ggtttttgcc gtgcgcaccg gctttttcaa aacctgcgtc ggcagccatg acggccgtga
    17641 gcaggtcacc ggctttcata tgtcgggtga gcttttgggg ctggatggca tctcggccaa
    17701 cgcccacggc tgcgacagca tcgcactgga agacagcgaa gtctgcgaac tgccgtttgc
    17761 gcgcatggaa agcctgggcc gcgacatccc cagtctgcaa caccattttt tccgtttgat
    17821 gagccgcgaa atcgtgcgcg accaatcggt gatgctgctg ctgggcaata tgaaggcgga
    17881 agaacgcctg gcggcgtttt tgctgaacct gtcgctgcgt ttttccaccc gcggctttgc
    17941 cgccaatgac tttatcctgc gcatgagccg cgaagaaatc ggctctttcc tggggctgaa
    18001 gctggaaacc gtcagccgca cgctgtcgcg ctttcaggcc aatggctgga tcaccgtcga
    18061 gcacaagcac atccagctgg tgcagccgac cgcgctgaaa gagctgattt ccggctgttc
    18121 gggcaacacc cagcaaaccc acgccatgta acaccacatt ggcctgaaca caccaacgcc
    18181 acgcccccgc tcaatgcggg ggcgtggcgt tggtattttt gcaacagatt tgtactgtac
    18241 aaaaacacct ggccggcgca caattttgtc tggattattt acccagtcga gccccgccat
    18301 gcaatacacc gacatcgccg tgattggcgc cggcctggcc ggcgtcagct gcgcccgcca
    18361 actggccgcc gccggccgcc aggtgaccgt gttcgacaaa agcaagggca tgggtgggcg
    18421 catggccacc cggcgcggcg atggcttcag cgtggaccat ggcgcgcaat acttcaccgc
    18481 ccagcagccg gattttcacg ccgaaatcca cgcctggcag acggctggcc tgtgcgcgcc
    18541 gtggcaggcg cggctggtgc aacatcgccc aaccggcccg gtggacggct acccggaaca
    18601 ccgctacgtc gccacgcccg gcatgagcag cctgtgccgt gcgttggcgg cagcgctgcc
    18661 cgtgcaactc aatcaccggc tgaccgcgct ccagcgccaa gcggacggtt ggcaactgag
    18721 ctttgccgac cagcccagct ggcgcgcccg gcaggtgctg ctggcggcgc ctgccccgca
    18781 ggccacagca ttgctggacg ccgcgccagc cttgcgcgcg ctggccgccg gcgtcgagat
    18841 ggaagcctgc tgggcggtga tgctgcacgc acaagcgccg ctggagatcg gctttgacgc
    18901 cgcctgggtg gagcacggcc cgctgcgctg gctggcgcgc aatagcagca agcccggccg
    18961 cagcagcgca gaaagctggt tgctgcacgc caccccggcc tggagcgccg cccatattga
    19021 tgccccggct gatgaagtcg ctggcttgct gcatgcggcg tttgccgagc tggtggacag
    19081 cgccgtcgat gcccgcaacc gcagcgcgca ccgctggctg tacgcccagg ccgcgcagcc
    19141 gctggacatc ggcagcgcct accaagctga actgggcttg ggcctgtgcg gggattggtg
    19201 tctgggtacg cgggtggaag acgcctggcg cagcggccgt catctggccg agcaagcact
    19261 ggccgcagct tgagcggcga gccggctcac cgcattggct tggcccagcc aagcaccgcg
    19321 cacaacatgt tcggcacatc cctggccagg gggttgagcg acgctaatta gccagcggta
    19381 gccgcagagt aaagcgcgcg ccgccttctg gcgcattatc cgcccgcagt tggccgccgt
    19441 gctgctcgac aatgccaaag ctgatcgaca ggcccagccc ggtgccttcg cccactggtt
    19501 tggtggtgaa aaacggctcg aacagccgtt gctgcacctc tggcgacagg cccggcccat
    19561 tatccgccac ggtgacggtg gcccagccat cggcctcggt cagccgcacg ctgacgcgcg
    19621 gggccgggtg gtgggcggtg gcgtccacgg cgttttgcac cagattgatc aacacctgct
    19681 gaatccgccc ggcggcgccg cacagcatca ccggcgcgtc tggcgcgtcc agcagcagct
    19741 gcacatgcgc cggcgcggct ttttccaccc agtgcgccga gcggcgcacg gcatcagcca
    19801 ggtcaaagcg ctcggcgccg ccccggtcgg tggcggaaaa gcgcttgagg ccatcgacaa
    19861 tcgccgccga gcgttcggcg ccttccatca agccttcaat cagcgacggc aaatcggcca
    19921 gaatggc
//
LOCUS       DAOPYX010000018        18451 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_3155_length_18451_cov_11.746847, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000018 DAOPYX010000000
VERSION     DAOPYX010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 18451)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18451)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18451
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3155_length_18451_cov_11.746847"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(68..592)
                     /locus_tag="PKN55_01900"
     CDS             complement(68..592)
                     /locus_tag="PKN55_01900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014088651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HNX70028.1"
                     /translation="MLPSEPFASPAAASFDTPLDMLTSCHQKVLRCCAQLDKLPGYLA
                     EHGVTPVVVTTLEGVLRYFDVAGPLHHADEERDLFPLLAARAPDSQPLLTEIAIEHIT
                     LEGMWARLREQLLAVNAGDAAALDTHLSQAFVQRYRGHIDHEEGELLPLAQQVLSTDE
                     LERIGGAMSARRRD"
     gene            709..1284
                     /gene="ampD"
                     /locus_tag="PKN55_01905"
     CDS             709..1284
                     /gene="ampD"
                     /locus_tag="PKN55_01905"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018746827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HNX70029.1"
                     /translation="MSTALTLGADGWIDQARRAPSPNCDAQTEPPHLLVIHHISLPPG
                     QFAGHDIERLFTNTLPAEGHPDYSWISQLRVSAHWLIRRDGELVQFVSAGQRAWHAGA
                     SCWRGRQTCNDFSLGVELEGTGDTPFTDAQYARLGELARTLAAALPIVEMTGHEHIAP
                     GRKSDPGPSFDWPRAQSACGLPVWFGDQSST"
     gene            complement(1269..3143)
                     /gene="kup"
                     /locus_tag="PKN55_01910"
     CDS             complement(1269..3143)
                     /gene="kup"
                     /locus_tag="PKN55_01910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011134128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HNX70030.1"
                     /translation="MSSASGHKPALPALTLAALGIVYGDIGTSPLYTLKECFSAHTHL
                     TPTVANVTGILSLIFWSIIVVVTLKYISFVLRADNKGEGGILTLAALAGRKLEGLPRS
                     LVLVLGLLGAGLFFGEVVITPAISVLSAVEGLEVVAPELDAYVLPLALGVLIALFLIQ
                     KSGTAKVGAFFGPIMGLWFLTLGALGVLGISKQPAVLQAVNPMLGLSFLADHGWASLL
                     ALGSVVLALTGAEALYADMGHFGRKPIRYAWFGLVLPALLLNYFGQGAHLLSNPEAVE
                     NPFFHLAPDWATMPLVALATAATVIASQAVISGVFSLTRQAIQQGLLSRLSIQHTSDH
                     EIGQIYIPAVNWALLGSVIIVVLAFQNSSALAAAYGIAVTGTMIITSILACTVARRQW
                     GWPLLATVALLSVLLVIDVPFFVANVLKLFSGGWLPLVIGLVMFVIMSTWRRGRELLF
                     QRLQEQALPLDSFIENLETYPPTRVQGTAVFLNSATLGVPHALLHNLKHNKVLHERIV
                     LMTVQNIDEPYVKTENRVQVIRLSNSFWQVVARYGFKEVPNVPEILDLCAQQGLEFEP
                     MDTSFFLSRETLISTDRPGMARWREKLFVLQSKNALRATDFFQIPANRVVEMGTQVEL
                     "
     gene            3387..4907
                     /gene="pap"
                     /locus_tag="PKN55_01915"
     CDS             3387..4907
                     /gene="pap"
                     /locus_tag="PKN55_01915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008952392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0043751 - polyphosphate:AMP
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006797 - polyphosphate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate:AMP phosphotransferase"
                     /protein_id="HNX70031.1"
                     /translation="MFESAELGHRIDKARFREESPALRQALLEAQYELRERGDFPVII
                     MLNGFDGAGRSETMNLLSSWLDPRLIQIHTRTSAFADEDDDNRGRPWLWRYWRALPPK
                     GRIGLMYGSWYSDILFGQVNGHLDSETAAHRVSEVLRLERQLVAEGALIIKLWLHLSR
                     EKQKKRLKALEHDPATRWRVRAQDWRHYKQYADIRRHAEHILRRTSTGEAPWVIVEGE
                     DDNYRHLTIGHVILQALRARLDAAPLTHPRAEAPPLLPPLDGLQLLDTLQLDQPMGKA
                     DYQRELEHWQARLNRLTRHPDFARRALVAVFEGNDAAGKGGAIRRIATALDARAYRIV
                     PIAAPSEDERAMPWLWRFWRQVPSHGNITIFDRSWYGRVLVERVEGFAREHEWLRAYG
                     EINDFEHQLADAGVMVVKFWLAISHDEQLARFQAREQTGFKRFKITEEDWRNREKWLA
                     YKDAVCDMIDRTSTQTAPWTLVEANNKHYARIKVLRTLCERLESQLDTTPEDDAAG"
     gene            complement(4967..5533)
                     /locus_tag="PKN55_01920"
     CDS             complement(4967..5533)
                     /locus_tag="PKN55_01920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX70032.1"
                     /translation="MKWPARPTGLPWRCLLVALSLALGLGIVWAGWRHWRVADARLSQ
                     QRTLAAHAQAQQAALQQRQQQHEHDTPLWHTLRQRGWLAPENRLAALDILAQLPRQPG
                     VQHARAQLGPRQRLSWPWPPDAPLAHSLLHIQLRLSHPRHLADMLAPLASLPGLMLPT
                     ACQWRRQAADDDTVDGECQLAWISQANE"
     gene            complement(5530..6921)
                     /locus_tag="PKN55_01925"
     CDS             complement(5530..6921)
                     /locus_tag="PKN55_01925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX70033.1"
                     /translation="MKAGKTHEWIIVLGDTAAWAWPLFASPAAPRRFAADAEGHAALL
                     RCLGESHGEVHSRVLIDLACETLHEQHTPALRWHDRRALAQQCRRNQFAADALVGWRW
                     RPGLADDTVLQLCGVPMQGSLPAWLHLLTHGAAPPVALYSASWLGEPLARTLGASHGP
                     ALLLQPGLASARLSWLQDGRVRHSRQVGPAGEDISQVLDFFASQPDYPAHGIPTLWQT
                     GEDDSLVHHPALAGFQRQQAPLESGWPDWLSAQLSRQPGDYRWPPGTSRPQLGQCSPR
                     SRRLTLAISLAAAVLGGGWFAWGEALNRQAAQLAEHTRALHASNQQLAASLGPAEVTQ
                     ARRAALNSLLILRTRWPRLGDDLDALAEQLARRPALSVTALHWRADPARLSQGSGAHV
                     QLELRADATLSARQALAELEQLLAACRQAGWNATLSHAPFDTRAEATLHSQSEEVVRF
                     VVQLTLPARKAGQ"
     gene            complement(6918..8111)
                     /locus_tag="PKN55_01930"
     CDS             complement(6918..8111)
                     /locus_tag="PKN55_01930"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HNX70034.1"
                     /translation="MRRYRFRAVHADGRVVHGVRQAANDAALAQRLARNGLTLLRARP
                     CRAARGLTASELAEWCFHLEQLLRAGVPLLDALGSVRDAQDHRRPRDAMAMLVDAVHD
                     GQPLSEAMAAQAESFSPLLCGLVQAGETAGELASTLARLADSLRRDEALRAHLRKSLL
                     YPAFVLVLVLLVAALMLTQVVPQLASLLASTGESLPWSTQALLATSALLLQYGAPLAV
                     AGLALAIGLPWLARQWPWLATARDQLALRLPLWGPLQHKRELARFAEALAMLSASGVP
                     LLDALRVGEAVIDNQVLRHGVSLARRHIANGSGLSDGFAAVGILPPLALRMLRVGENS
                     GELARALAQVSDSYQREVRLASERLLTLLEPTLTVLLGGLLLWMVSAVLGPVYSALTR
                     LGASA"
     gene            complement(8115..9782)
                     /locus_tag="PKN55_01935"
     CDS             complement(8115..9782)
                     /locus_tag="PKN55_01935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018746648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HNX70035.1"
                     /translation="MSALAARLGDTLLARGWLDADQLDIAQAEVRAGRPLEQALAQLG
                     FVPDGVLRDTLAELLGLDSLEARALVADSTALAVVPAHWARQLRVLPLRYAPANQTLT
                     IASARPADLRAHDQLVAASQGQVARVHVQLADEADILQALDRAYGAPDTLSAVLTEWR
                     SGQQDGEAPAVRLAQALIEDAARLGASDIHLEPEAGFVRIRYRIDGVLRPARSLRKAH
                     WPPLLVRLKVMSGLNIAEQRAPQDGQLSMQVAGRTLDIRVACLPTLHGENLSLRLFDR
                     ARGLPTLASLTTATQHAQLTRMLARPEGMLLVTGPTGAGKTTTLYAMLAHLNDSTVNI
                     MTLEDPVEYPLPMVRQTPVNEALKLDFASGIRALMRQDPDILLVGEIRDEATARMAFR
                     AAMTGHLVLSTLHAHSALGAIPRLLDLGVGPDMLAGNLIGVVGQRLVRALCPACRQPV
                     PTDSRARRLLGAAADQAMLYRARGCPLCRHDGHRGRLPVLEILDIDSELDAMIGRRAP
                     LADLRRHAGQQGFIPLAEAMRSRVLAGEVALDEALRVVDLGHRAGEG"
     gene            complement(9779..10171)
                     /locus_tag="PKN55_01940"
     CDS             complement(9779..10171)
                     /locus_tag="PKN55_01940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX70036.1"
                     /translation="MPAATPADPIALAEPAPLARSTPITDESIAPPAPATTPATDPWA
                     RPAGLRRDPTVARTTNALASAPDTEAWQTGNQLLASGQWWPAIAAYQRAYAHTPDPAL
                     AHNLALALTAAGEHVAAQHWQQRAGEHP"
     gene            complement(10516..12267)
                     /locus_tag="PKN55_01945"
     CDS             complement(10516..12267)
                     /locus_tag="PKN55_01945"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX70037.1"
                     /translation="MLLTACQHTPVPAPSEHLMRAEAPAMRPPAPLPPPAPAAAATPT
                     FSLNVIASPVQQILINLARDAKVNMDVHPAISGVVTLHAIRQPLPALLERIAQQVDIR
                     WRHEGDTYLITPDLPYVESYMVDYVAIEREARGESGVVTSVVSASANQGSDARASQSG
                     TQLRSSSQHRFWRSLLSSLEELLRREDETVQIVTIEREQEIRDTTTHTDSQTRSRKLN
                     PPSANQGAAPENGVTVRDGEHSKFSHTRVQPGQRQTRSEDKTVRVTLHLETGVLAVRA
                     TRRQHALVGRFLRQIAHSARRQVLIEATIVEVELTREFETGVDWSRLTGSASPQFVQN
                     SSNLLSDTALRGTMPLDRALGANPVSLLYYHSGSLTSAVKLLEHFGRTRVLSSPKLMA
                     LNNQAALLKVVRDMPYFTLKVRRTEGGADKPDRVDYESTLHTVPEGIVLSVQPQISAN
                     GEVSLHVRPTITQITGYVSDPAVTLADARVNSPIPRVQVRELESTLRLMSGQIGVLGG
                     LIQDRLDNQSDGLPGLGMLDGIGRLFSHKTDRLVRTELVVFLRPTLVEHPAVHGGDLA
                     GLAGTLPDAGFFNGSGR"
     gene            12673..13485
                     /locus_tag="PKN55_01950"
     CDS             12673..13485
                     /locus_tag="PKN55_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011134004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNX70038.1"
                     /translation="MEPDAFIELDKVSFAYGSRRILDGVSLRIARGKLAAIMGGSGSG
                     KTTLLRLISGQVKPDSGRVWVDGQDVAKLNTAGLYAMRRRMGMLFQFGALFTDLSVQD
                     NVAFPLREHTGLPDTMIRDLVLMKLQAVGLRGAAGLMPAELSGGMARRVALARAIALD
                     PDLMLYDEPFTGLDPIALGTIAALIKRLNDALGATAVMVTHDVHVSLNVVDEVYFVAD
                     GKIVASGTPDQVRHSADPFVRQFVDGQPDGPVRLHYPAEDYASQLGLGGRHG"
     gene            13478..14266
                     /gene="mlaE"
                     /locus_tag="PKN55_01955"
     CDS             13478..14266
                     /gene="mlaE"
                     /locus_tag="PKN55_01955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HNX70039.1"
                     /translation="MADSSSRNPFAAFGHRLIEAVWKLGFVCRFFAAILRDSGASLRR
                     FPLVIREIYFAGVLSMIIIVVSGLFVGMVLGLQGYSTLARFGSADALGALVALSLLRE
                     LGPVLAALLFASRSGSAITAEIGLMKATEQLDAMAVMAVNPIARVVAPRFWAGVLSMP
                     VLAAIFNVMGIFGGYFVGVTLIGLDHGTFWSQMQNAVDWHTDIVNGLIKSLVFGAAVS
                     LIAVFEGFDAKPTAEGVSSATTRTVVTSALTILALDFVLTAFMF"
     gene            14292..14774
                     /gene="mlaD"
                     /locus_tag="PKN55_01960"
     CDS             14292..14774
                     /gene="mlaD"
                     /locus_tag="PKN55_01960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007343933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HNX70040.1"
                     /translation="MKRATIDLWVGIFAALGCAAVLFLALKTANLTVGSGSNGTYSLY
                     AEFDNIGGLKVKAPVKMAGVVVGRVTDIRLDNKSFRARATLEMDKQYQFSRDVSAEIL
                     TSGLLGEQYIGLLQGGDPDNLAAGDTIKLTSSALVLEQLIGKFMLNAAEKPAAPADSH
                     "
     gene            14840..15469
                     /locus_tag="PKN55_01965"
     CDS             14840..15469
                     /locus_tag="PKN55_01965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNX70041.1"
                     /translation="MKNLLGKLLICCAFVSFSLHAMAETPDELVRDTSRQVLDVLKRD
                     NGKNTKNIRAEVEALVLPKFDFTRMTALAVGRGWRDATPDQQKQLTDQFRMLLVRTYS
                     STMTRFKNAQITVQASAPAADAREVTVRSDVELPANAGDKRSAQVDYTLIKTPTGWRV
                     YNVSIEGASLVTIYRNNFNDILRQGGPDALIQSLKDKNDALSGGKHASN"
     gene            15456..15728
                     /locus_tag="PKN55_01970"
     CDS             15456..15728
                     /locus_tag="PKN55_01970"
                     /inference="COORDINATES: protein motif:HMM:NF024857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNX70042.1"
                     /translation="MPATDDGLTLALSGPVTFAHTARLARELRPRAAAARVLDWSAVT
                     QVDSSAVALILELARAAGHGHTLRQPPAGLSSLITLYGVEDLVRAP"
     gene            15712..16479
                     /locus_tag="PKN55_01975"
     CDS             15712..16479
                     /locus_tag="PKN55_01975"
                     /inference="COORDINATES: protein motif:HMM:NF016236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ family lipoprotein"
                     /protein_id="HNX70043.1"
                     /translation="MFAHRDPRRARRVLPALLAVSLLAGCAAATPQNRFDPYEAANRK
                     VHAFNDVVDRAALQPLARGYRAVTPEPARAAVSNVFGNLEDVVSVVNHLLQGQFARAS
                     SDLMRVALNSTLGLGGLIDIATPAGLPSYKTGFGETLGRWGYQNSAYVVLPLLGPSTV
                     RDTVGRAADIASNPRTYLLPEPADANVATGVNLLSQRANALELTDAADEAALDKYSFT
                     RDAYLQYRRTRIGAADSPAMNDEPSIDALVPPEAPAK"
     gene            16505..16657
                     /locus_tag="PKN55_01980"
     CDS             16505..16657
                     /locus_tag="PKN55_01980"
                     /inference="COORDINATES: protein motif:HMM:NF023816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX70044.1"
                     /translation="MPRCKWPTYAYVLENGQVHMHGPARELADDPRVVEAYLGLGSKH
                     QAMLSS"
     gene            16897..17958
                     /locus_tag="PKN55_01985"
     CDS             16897..17958
                     /locus_tag="PKN55_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003146062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HNX70045.1"
                     /translation="MHSLSPDYLAKLRFDAQQLATLRALGECRGKQQLFVVQSPEVLS
                     DLRQVAVVESTESSNRLEGVVVAPHRLKSLVLKNATPQSRSEQEVAGYRDALGLIHES
                     GEQMPFSEGTVLQLHGMLYRYMPQSGGHWKATNNDIVERHPDGSSRIRFRPVAAHLTP
                     IAVADLIARYRFALDQHLADPLVLAPLAILDFLCIHPFPDGNGRMARLLTLQLLYHFD
                     YAVGRFISLERIFEESKESYYETLEVSSQGWHEAAHNIAPWLNYFWGALLRAYKEFEE
                     RVGAIEHGRGAKGERIRAEILKRQLPFSISDIEEACPGISRDMVRVVLRAMKMEGLIT
                     PTGKGRAAKWLHIEAEEQT"
BASE COUNT         3298 a         5892 c         6016 g         3245 t
ORIGIN      
        1 aggcgtccat cctgacaaag cctgatggac gcttcgggtc gtgtcaatgt atttaacttg
       61 agtgggctca gtcgcgacgg cgtgcgctca tggcgccgcc aatccgctcc agttcgtcgg
      121 tgctcagcac ctgctgcgcc agcggcagca actcgccttc ttcgtggtcg atatgaccgc
      181 gatagcgctg cacaaaagcc tggctcaggt gggtgtccag tgcggccgcg tcgccagcgt
      241 tcaccgccag cagttgctcg cgcagtcgtg cccacatgcc ttccagggtg atgtgctcga
      301 tggcgatttc ggtcaagagc ggctggctgt ccggcgcgcg cgcggccagc agcggaaaca
      361 aatcgcgctc ttcgtcggcg tggtgcagcg ggccggccac gtcaaaatag cgcagcacgc
      421 cttccagcgt ggtgaccacc accggggtga cgccatgctc ggccagatag ccgggcagtt
      481 tgtccagctg ggcacagcag cgcagcactt tttggtggca gctggtcagc atgtccagcg
      541 gcgtgtcaaa actggccgcc gccgggctgg cgaagggttc ggatggcaac atggcaaaac
      601 tccaaaagtc aaaacggttt gcccaagccg cccaagcggg ctgggcaaac agttctgccg
      661 ggcgacgcgg tatcatcgcc gtcctgattg tctgtttcga gcatcgatat gtccactgcc
      721 ctgacgctgg gcgccgacgg ctggattgac caggcccggc gcgcgccctc gcccaattgc
      781 gacgcccaga ccgagccgcc gcacctgctg gtgattcacc atatcagcct gccgcccggc
      841 cagtttgccg gccacgacat cgagcggctg ttcaccaaca ccttgccggc agagggccac
      901 ccagactaca gctggatttc ccagttgcgg gtgtcggcgc actggctgat tcgccgtgat
      961 ggcgagctgg tgcagtttgt cagcgccggc cagcgcgcct ggcatgccgg cgcctcttgc
     1021 tggcgtggcc ggcaaacctg caatgatttc tcgctgggcg tcgagctgga aggcaccggc
     1081 gacacgccgt tcaccgatgc gcaatatgcc cggttgggcg aactggcgcg cacgctggcg
     1141 gcggccttgc cgattgtcga gatgaccggt cacgagcata ttgcgccggg gcgcaaaagc
     1201 gaccccggcc cctcgtttga ctggccgcgc gcgcaaagcg cgtgcgggct gcccgtctgg
     1261 tttggcgatc agagctcgac ctgagtgccc atctccacca cccggttggc cggaatctgg
     1321 aagaaatcgg tcgcgcgcag ggcgtttttg gattgcaaga caaacagttt ttcccgccag
     1381 cgcgccatgc cggggcggtc ggtggagatc agcgtttcgc gcgacaggaa gaacgaggtg
     1441 tccatcggct caaactccag cccctgctgc gcgcacaggt caagaatctc cggcacattg
     1501 ggcacttctt taaagccgta gcgcgccacc acctgccaga agctgttgga caggcggatg
     1561 acttgcaccc ggttttcggt tttgacatac ggctcgtcga tgttctgcac cgtcatcagc
     1621 acaatgcgct cgtgcagcac cttgttgtgc ttgaggttgt gcagcagcgc atgcggcacg
     1681 cccagcgtgg cgctgttaag aaacaccgcc gtgccctgca cgcgggtggg cgggtaggtt
     1741 tccaggtttt cgataaacga atccagcggc agcgcctgtt cctgcaagcg ctggaacagc
     1801 agctcacggc cacggcgcca ggtgctcatg atcacgaaca tcaccaggcc aatcaccaac
     1861 ggcagccagc cgccagagaa cagcttgagc acattggcga caaagaacgg cacgtcaatc
     1921 accagcagca cgctcaacag cgccacggtg gcaagcagcg gccagcccca ttgccggcgc
     1981 gccaccgtgc aggccaggat ggaggtgatg atcatggtgc cggtgacggc aatgccgtag
     2041 gcggcggcca gggcgctgga attctggaaa gccagcacca cgatgatcac cgagccgagc
     2101 aaggcccagt tcaccgccgg aatgtaaatc tggccgattt catggtcgga ggtgtgttga
     2161 atggacaggc gcgacagcag cccttgctgg atggcctggc gggtgagcga gaacaccccg
     2221 gaaatcaccg cctgcgaggc aatcaccgtt gccgcagtgg ccagcgccac caagggcatg
     2281 gtggcccagt ccggcgccag gtggaagaac gggttttcca ccgcctccgg gttgctcagc
     2341 aaatgcgcgc cctggccaaa gtagttcagc agcagggccg gcagcaccag gccaaaccag
     2401 gcatagcgaa tcggcttgcg gccaaagtgg cccatatcgg cgtacagcgc ctcggcgccg
     2461 gtcagcgcca gcaccaccga gcccagcgcc agcaggctgg cccagccatg gtcggccaga
     2521 aagctcaggc ccagcatcgg attgaccgct tgcagcacgg ccggctgttt gctgatgccc
     2581 agcacgccca gcgcgcccag ggtgagaaac cacagcccca tgatcgggcc aaaaaacgcc
     2641 cccaccttgg cagtgccgct tttttgaatc aaaaacagcg caatcagcac gcccagcgcc
     2701 agcggcagca cataggcgtc cagctcgggc gccaccacct ccagcccttc gaccgcagac
     2761 agcaccgaaa tggccggggt gatcaccacc tcgccaaaga acaggcccgc gcccaacagg
     2821 ccaagcacca gcaccagcga gcgcggcaag ccttccagct tgcggccagc cagcgccgcc
     2881 agggtgagga tgccgccttc gcctttattg tcggcgcgca gcacaaaaga aatgtatttc
     2941 agggtgacca ccacgatgat cgaccagaaa atcagcgaca ggatgccggt gacgttggcg
     3001 acggtggggg tcaggtgggt gtgggcgctg aagcattctt tcagcgtgta cagcggactg
     3061 gtgccaatat cgccgtagac aatgccaaga gcggcaaggg tcagcgcagg cagcgccggt
     3121 ttgtggccgg aagcagagga catggaaaac gccagtgatg tgccaaactg gaatggtagt
     3181 aagtttacgg gggcgcgcca acacctctgt gcaccgggcg cgacccggac acctcttaaa
     3241 agttcattcc ggctctattg cgttgcacaa tcgcgacaaa aacccggttt gggcggttca
     3301 catgcccatt tatggggctg gcctgcgact gcctgatgcg ctgctgctgg agtatgctgg
     3361 cgcaatcttg ccaccggagt tgcaccatgt ttgaatccgc cgagctgggc catcgcatcg
     3421 acaaggcgcg ttttcgtgaa gaaagcccgg cgctgcgcca ggcgctgctg gaggcgcaat
     3481 acgagctgcg cgagcgcggg gattttccgg tgatcatcat gctcaacggc tttgacggcg
     3541 ccgggcgcag cgaaaccatg aatctgctgt cgtcctggct ggacccgcgg ctgatccaga
     3601 ttcacacccg cacctcggcc tttgccgacg aagacgacga caatcgcggc cggccctggc
     3661 tgtggcgcta ctggcgcgcg ctgccaccca aggggcgcat cggcctgatg tatggctcgt
     3721 ggtattccga cattctgttt ggccaggtca acggccacct ggacagcgaa accgccgccc
     3781 accgcgtcag cgaagtgctg cggctggaac gccaactggt ggccgaaggc gcgctgatca
     3841 tcaagctgtg gctgcacctg tcgcgcgaga aacagaaaaa acgcctgaaa gccctggagc
     3901 acgacccggc tacccgctgg cgagtgcgcg cgcaggactg gcggcattac aagcaatacg
     3961 ccgatatccg tcgccacgct gaacacatcc tgcgccgcac cagcacgggc gaagcgccat
     4021 gggtgattgt cgagggcgag gacgataatt accgccacct gaccatcggc cacgtcatct
     4081 tgcaagcctt gcgcgcccga ctggacgccg cgccactgac ccacccacgc gccgaagcgc
     4141 cgcccttgct gccgccactg gacggcctgc agctgctgga caccctccag ctggatcaac
     4201 cgatgggcaa ggccgactat cagcgcgagc tggaacactg gcaggcgcgg ctgaaccggc
     4261 tgacccgcca cccggatttt gcccgtcgcg cgctggtggc ggtgtttgag ggcaatgacg
     4321 ctgccggcaa gggtggcgcc atccgccgca ttgccaccgc gctggacgcc cgtgcctatc
     4381 gcatcgtgcc gattgccgcg ccctccgaag acgaacgcgc catgccctgg ctgtggcgct
     4441 tctggcgaca ggtgcccagc cacggcaaca tcaccatttt tgaccgctcc tggtatggcc
     4501 gtgtgctggt ggagcgggtg gaaggctttg cccgcgagca cgaatggctg cgcgcctacg
     4561 gcgagatcaa cgacttcgag caccagttag ctgacgccgg ggtgatggtg gtcaagttct
     4621 ggctggccat cagccacgat gaacaactgg cgcgctttca ggcgcgcgaa caaaccggct
     4681 tcaagcgctt caagatcacc gaggaagact ggcgcaaccg ggaaaaatgg ctggcctaca
     4741 aagatgcggt gtgcgacatg atcgaccgca ccagcacaca aaccgcgccg tggaccttag
     4801 tagaagccaa taacaagcac tacgcccgca tcaaggtgct gcgcacgctg tgcgagcggc
     4861 tggaaagcca gttggacacc acgccggaag acgacgccgc tggctgatgg cgtcagggcc
     4921 gagtggccgg tgacgttgaa acaccacggc gccggcgagc aggtggctac tcgttggcct
     4981 ggctgatcca ggccaactga cattcgccgt ctaccgtgtc gtcatctgcg gcctgtcgcc
     5041 gccactggca ggcggtgggc aacatcagac cgggcaagct ggccagcggc gccagcatgt
     5101 cggctaagtg gcggggatga ctgagtctca gctggatgtg caacaggctg tgcgccagtg
     5161 gggcatcggg cggccacggc caggacagcc gctgacgtgg gcccagttgg gcgcgggcgt
     5221 gctgtacgcc aggctggcgc ggcagctggg ccagaatatc cagggcggcc agccggtttt
     5281 ctggcgccag ccagccgcgc tggcgcaggg tgtgccacaa gggcgtgtcg tgctcgtgct
     5341 gctgctggcg ttgttgcaag gctgcttgct gcgcctgggc gtgggcggcc agggtgcgct
     5401 gctggcttag ccgggcgtcg gccacccgcc agtggcgcca gccggcccag acgatcccca
     5461 ggcccagcgc cagactgagc gccaccagca ggcagcgcca gggaaggccg gtgggtcggg
     5521 cgggccattt cattggcccg cctttcgggc cggcagggtc agctgcacga caaaccgcac
     5581 gacctcctcg ctttggctgt gcaaggtggc ttccgcgcgg gtatcgaagg gggcgtggct
     5641 gagcgtggca ttccagccag cttgccggca ggcggccagc aactgctcca gctcggccag
     5701 cgcctgacgg gcggaaaggg ttgcgtcggc gcgcaattcc agttgcacat gggcgccact
     5761 gccctgactg agccgggcag ggtcggcgcg ccagtgcaag gccgtaactg acagcgctgg
     5821 ccggcgggcc aactgttcgg ccagggcatc cagatcgtcg cccaggcgtg gccagcgcgt
     5881 gcgcaatatc agcaggctgt tcagggcggc gcggcgtgcc tgcgtgactt ctgccgggcc
     5941 caggctggct gccagttgct ggttgctggc gtgcagggcg cgggtgtgct cggccaactg
     6001 ggccgcctgc cggttcagcg cttcgcccca ggcaaaccag ccaccgccca acacggcagc
     6061 ggccagactg atggccaggg tcagccggcg gctgcggggc gaacattgcc ccagttgtgg
     6121 ccgcgatgtg ccgggtggcc agcgataatc gcccggctgg cggcttagct gtgcgctcag
     6181 ccagtccggc cagccggatt caagcggtgc ttgctggcgc tgaaagcccg ccagggctgg
     6241 atggtgaacc agactgtcgt cctccccggt ttgccagagg gtggggatgc cgtgggccgg
     6301 gtaatccggc tggctggcaa aaaaatccag cacctggctg atgtcctccc cggccgggcc
     6361 gacctggcgg ctgtggcgca cccggccatc ctgtagccag ctcagtcggg cgctggccaa
     6421 gccaggttgc aacagcagcg ccgggccgtg gctggcgccc agcgtgcgcg ccagcggctc
     6481 gcccagccaa ctggctgaat acagcgccac aggcggggct gcgccatgtg tcagcagatg
     6541 cagccaggcg ggcagtgagc cctgcattgg cactccacac agctgcaaca cggtgtcgtc
     6601 tgccaaaccc ggccgccagc gccagcccac cagcgcatcg gcagcaaact ggttgcgccg
     6661 gcattgctgc gccagtgcgc gtcgatcatg ccagcgcaag gccggtgtgt gctgctcgtg
     6721 cagggtttcg caggccaggt cgatcagcac ccggctgtgt acttcaccat gactctcgcc
     6781 caagcagcgc aatagcgcgg cgtggccttc ggcgtcggcg gcaaaacggc gtggggcggc
     6841 tgggctggca aacaggggcc aggcccaggc ggcggtatcg cccagcacga tgatccattc
     6901 gtgagttttg ccggccttca tgcgctggcg cccagccggg tgagcgcgct gtataccggg
     6961 ccgagtacag cgctgaccat ccacaacagc aggccgccca gcagcacggt cagggtgggt
     7021 tccagcaggg tcagcagccg ctcgctggcc agacgcacct cccgctgata actgtcgctg
     7081 acctgggcca gcgcccgagc cagctcgcca ctgttttctc ctactcgcag catgcgcaag
     7141 gccagcggcg gcaagatgcc gacggcggca aagccatcgc tcaggccgct gccgttggcg
     7201 atatggcgac gcgccaggct gacgccatga cgcagcactt ggttgtcgat gacggcctcg
     7261 cccacccgca aggcatccag caagggcacg ccggaggcgc tcaacatggc cagggcctcg
     7321 gcaaagcggg ccagctcacg cttatgctgc aaggggcccc aaagtggcag gcgcaaggcc
     7381 aactgatcgc gcgctgttgc cagccagggc cactgtcgcg ccagccaggg caggccgatt
     7441 gccagcgcca gcccggccac ggccaggggc gcgccgtact gcagcagcag ggctgatgtg
     7501 gccagcagcg cctgggtgct ccacggcagt gattctccgg tgctggccag cagggaggcc
     7561 aactggggca ccacctgggt cagcatcagg gcggccacca gcagcaccag caccagcacg
     7621 aaggccgggt agagcaggct tttgcgcaga tgagcgcgca aggcctcgtc gcgacgcagg
     7681 ctgtcggcca gacgcgccag ggtggaggcc agttcgccag cggtttcacc ggcctgcacc
     7741 aggccgcaga gcaggggtga aaagctttct gcttgcgcgg ccatggcttc ggacaggggc
     7801 tggccatcgt gcaccgcgtc caccagcatg gccatggcgt cgcgcgggcg acgatggtct
     7861 tgggcatcac gcacactgcc cagcgcatcc agcagcggca ccccggcgcg cagcagttgt
     7921 tccagatgaa aacaccattc cgccagttcg ctggcggtca gcccacgtgc cgcccggcat
     7981 ggcctggcgc gcagcaaggt caggccattg cgcgccaggc gctgcgccag cgcggcatcg
     8041 ttggctgcct gacgcacgcc gtgcaccacc cggccatcgg catgcacggc acgaaaccgg
     8101 tagcggcgca tcgctcagcc ctcaccggcc cggtggccca ggtccaccac gcgcaaggcc
     8161 tcgtccaggg ccacttcgcc ggccagcacc cggctgcgca tggcctcggc cagcgggatg
     8221 aagccctgtt ggccggcatg gcggcgcagg tcggccagcg gcgcgcgtcg gccgatcatg
     8281 gcgtccagct cactgtcgat gtcgaggatt tccagcactg gcagccggcc acgatggcca
     8341 tcgtgccggc acagcgggca gccacgggca cggtagagca tggcctggtc ggccgctgca
     8401 cccagcaagc ggcgcgcccg gctatcggtg ggcaccggct gccgacaagc ggggcacagc
     8461 gcccgcacca gtcgctggcc caccacgcca atcaggttgc cggccagcat gtccggcccg
     8521 acccctaaat ccagcaggcg gggaatggcg cccagcgccg aatgggcgtg cagggtgctg
     8581 agcaccagat ggccggtcat cgccgcccgg aacgccatgc gcgcagtggc ttcgtcgcgg
     8641 atttcaccca ccagcagaat gtccgggtcc tggcgcatca gcgcgcggat accgctggca
     8701 aaatccagct tgagggcttc attcaccggt gtttgccgca ccatgggcag tggatattcc
     8761 accgggtctt ccagcgtcat gatgttgacc gtgctgtcat tcagatgcgc cagcatggcg
     8821 tacagcgtgg tggttttgcc ggcgccagtg gggccggtga ccagcagcat gccttccggg
     8881 cgcgccagca tgcgggtgag ctgggcatgc tgggtggcgg tggtcaggct ggccagtgtg
     8941 ggcaggccgc gggcacggtc gaacagccgc aggctgaggt tttcgccgtg cagggtgggc
     9001 aggcaggcca cccggatgtc cagggtgcgg ccggccacct gcatggacaa ctggccatcc
     9061 tgcggggcgc gttgctcggc aatgttgagc ccactcatca ccttcagccg caccaacaag
     9121 gggggccagt gcgccttgcg caggctgcgc gccgggcgca gtacgccgtc gatgcggtag
     9181 cggatgcgta cgaagcccgc ttcgggctcc aggtggatat ccgatgcgcc caggcgggcg
     9241 gcatcttcaa tcagtgcctg tgccagccgc acggcagggg cttcgccatc ctgctggccg
     9301 ctgcgccatt cggtcagtac ggcgctcagg gtatccgggg cgccataggc gcggtccagc
     9361 gcttgcagaa tgtcggcttc atcggccagt tgcacatgca cccgcgccac ctgtccctga
     9421 ctggccgcca ccagttgatc gtgcgcccgc aggtcggcgg gccgggcgct ggcgatggtc
     9481 agggtctggt tggccggcgc atagcgcagg ggcagcacgc gcagctggcg cgcccagtgt
     9541 gcgggcacca ccgccagtgc ggtgctgtcg gccaccagtg cccgcgcttc caggctatcc
     9601 aggcccagca actctgccag ggtgtcacgc agcacgccat cgggcacaaa gcccagttgc
     9661 gccagcgcct gctccagtgg tcgcccggcg cggacttcag cctgggcgat gtccagttgg
     9721 tcggcatcca gccagccccg cgccagcaag gtgtcgccca gccgggccgc cagcgcgctc
     9781 atgggtgttc tcccgcccgt tgctgccagt gctgggcggc aacgtgctcg cctgcggcgg
     9841 tcagcgccag cgccagattg tgcgccagcg ccggatcggg ggtgtgggca taggcacgtt
     9901 ggtaagcggc gatggctggc caccattggc cgcttgccag tagctggttg ccggtttgcc
     9961 aggcctcggt gtcgggggcg ctggcgaggg cgtttgtcgt tctggcgaca gttggatccc
    10021 gacgcaagcc ggccgggcgt gcccatgggt cggtggccgg ggtggtggct ggcgctggcg
    10081 gggcgatcga ctcgtcggtg attggggtcg acctggccag cggggcgggt tcggccaggg
    10141 cgatcgggtc agccggcgtg gcagctggca ttggcgctgc cgtggcctgg gtcggggttt
    10201 gggcttcaat aatgggagaa aatgcgatct gccagtaagc gaccgccacg gccaggctga
    10261 tgacggccag gccaatccat gcgccagtga ctttccggcc atattgccat cgccccacgg
    10321 gtgggcgggg ggcggcagcc actggtggcg gtgtgttgac ggcggcggtc aatgcggcga
    10381 ctggcgggct ggtcggtgtg gggctggcca ccgctggtgc cgttggctgg gcctgcggtg
    10441 gcggactgat ggagggggct gcgggcggat gcagcgatgc tcctggcacg ccaagatgcc
    10501 agtcggaggg gtgactcatc gtccgctccc gttgaaaaac ccggcatcgg gcagtgtgcc
    10561 ggccagcccg gccagatccc cgccatgcac agcagggtgt tccaccaggg tggggcgcaa
    10621 aaacaccacc agttcggtgc gcaccagccg gtcggttttg tgactgaaca agcggccaat
    10681 gccgtccagc atgcccaggc cgggcaggcc gtcactttga ttatccagcc ggtcctggat
    10741 cagtccgccc aatacgccaa tctggccact catcagccgc agggtggatt ccagctcgcg
    10801 cacttgtacg cgcggaatcg ggctgttcac ccgagcgtcg gccagcgtca ccgccgggtc
    10861 gctgacatag ccggtgatct gggtgatggt ggggcgcaca tgcagcgaga cttcgccatt
    10921 ggcgctgatt tgtggctgca cgctgagcac gatgccttcg ggcacggtat gcagcgtgct
    10981 ttcgtagtcc acccggtcgg gtttgtcggc gccgccctcg gtgcggcgca ccttgagggt
    11041 gaaatacggc atgtcgcgca ccactttcag cagtgctgcc tgattgttca aggccatcag
    11101 cttggggctg gacagcaccc tggtgcggcc aaaatgctcc agcagcttca ccgccgaagt
    11161 tagcgagccg ctgtggtaat acagcaaaga taccggattg gcccccagcg cccgatccag
    11221 cggcatcgtg ccgcgcaggg cggtgtctga cagcagattg ctgctgtttt gtacaaactg
    11281 cggcgaagcg ctgccggtca gccgcgacca atccaccccg gtttcaaact cgcgggtcag
    11341 ttccacttcg acaatcgtgg cctcgatcaa cacctggcgg cgggcgctgt gagcaatctg
    11401 gcgcagaaag cgcccaacca gcgcgtgctg gcgacgggtg gcgcgcaccg ccagcacccc
    11461 ggtttccagg tgcagggtca cccgcacggt tttgtcttcg ctgcgcgttt gtcgctggcc
    11521 gggctgtacg cgggtgtggc tgaacttgct gtgctcgcca tcgcgcacgg tgacgccatt
    11581 ttcgggcgca gcgccctggt tggctgatgg cggattcagc ttgcgcgagc gggtctggct
    11641 gtcggtgtgg gtggtggtgt cgcggatttc ctgttcgcgc tcgatggtga cgatctgcac
    11701 ggtttcgtcc tcacggcgca acagctcttc caggctgctc agcagcgaac gccaaaagcg
    11761 atgctggctg ctgctgcgca actgggtgcc ggattggctg gcacgggcat cgctgccctg
    11821 gtttgcgctg gccgacacca ctgacgtgac tacgccgctt tcaccgcgcg cctcccgctc
    11881 aatggccacg taatcgacca tatacgattc gacatagggc aaatcagggg taatcaggta
    11941 ggtgtcgccc tcgtgtcgcc agcggatgtc cacctgctgg gcaatgcgtt ccagcagtgc
    12001 tggcaagggc tggcgaatgg cgtgcagggt gaccacgccg ctgatggccg gatgcacgtc
    12061 catgttcacc ttggcgtcac gcgccagatt gatcaggatt tgctgtaccg gcgaggcgat
    12121 gacattcagg ctgaaggtgg gggtggccgc tgccgctggg gctggcggag gcagtggggc
    12181 tggtggacgc atggccggcg cttcggcgcg catcaggtgc tcgctgggcg ccgggacagg
    12241 cgtgtgctgg caggccgtca gcagcatggc acacagccag aaggcccgct tcattgtctg
    12301 gacctgctgc atggcaacat gccaacaggg aacctaatgt accgatgctc gctccatctc
    12361 atgcacactg caacacacgg cattgttgac cccctggcca agctggcttt gcacgcactg
    12421 ataacgttag aatggccggc cggcttccat gtctgcgtgt atcgccagtc tctggcattg
    12481 cgcgcaatgg gcacttcgcg atcggctctg gctatcttaa tagcgtttta cgctttcggc
    12541 atagcccgat gcgcatgaaa cggcaatttt attgccgtga ggtgcaaaaa agccgcactt
    12601 ttccatggcg tgtgcgcatc agcacgcgcc gggcctgttt ttcctgcaac cgcaacgaga
    12661 ttgacacaca cggtggaacc agacgctttc attgagctgg acaaggtcag ctttgcctat
    12721 ggctcgcgcc gcattctgga cggcgtcagc ctgcgcattg cgcgcggcaa gctcgccgcc
    12781 atcatgggcg gcagcggcag tggcaaaacc accttgctgc ggctgatttc cggccaggtc
    12841 aaacccgaca gcggccgcgt ctgggtggat ggccaggacg tggccaagct caacaccgcc
    12901 ggcttgtatg ccatgcgccg gcgcatgggc atgctgtttc agtttggcgc gctgtttacg
    12961 gatttatccg tgcaggacaa cgtcgccttt ccgctgcgcg aacacaccgg cctgccagac
    13021 acgatgattc gcgatctggt cttgatgaaa ctgcaagccg tcggcctgcg cggcgcggcc
    13081 gggctgatgc cggccgagct gtctggcggc atggcgcggc gggtggcgct ggcgcgcgcc
    13141 attgcactcg accccgacct gatgctgtac gacgagccct tcaccggcct ggaccccatc
    13201 gccctgggca ccatcgccgc gctgatcaag cgcctgaacg acgcgctggg cgccacagcg
    13261 gtgatggtga cccacgatgt gcatgtgtcg ctgaacgtgg tggacgaggt gtatttcgtc
    13321 gccgacggca aaattgtcgc cagcggcacg cccgatcaag tgcgccacag cgccgacccg
    13381 tttgtgcgcc aatttgttga tggccagccg gacggcccgg tgcgcctgca ttacccggcc
    13441 gaggattacg cctcgcagct gggcctggga ggccgccatg gctgattctt cctcgcgcaa
    13501 cccatttgcc gcgtttggcc accgcctgat tgaagcggtg tggaagctcg gttttgtctg
    13561 ccgctttttc gccgctatcc tgcgcgactc tggcgccagc ctgcgccgct ttccgctggt
    13621 gattcgcgaa atctattttg ccggcgtgct gtcgatgatc atcatcgtgg tgtccggcct
    13681 gtttgtcggc atggtgctgg gcttgcaggg ctattccacc ctggcgcgct ttggctcggc
    13741 cgacgcgctg ggcgcgctgg tggcattgtc cttgctgcgt gaactcggcc cggtgctggc
    13801 ggcgctgttg ttcgccagcc gcagcggctc ggccatcacc gccgaaatcg gcctgatgaa
    13861 ggccaccgaa caactggacg ccatggcggt gatggcggtc aacccgattg cccgcgtggt
    13921 ggcgccgcgc ttttgggccg gcgtgctgtc catgccggtg ctggcggcca tctttaatgt
    13981 gatggggatt tttggcggct attttgtcgg cgtcaccctg atcggccttg atcacggcac
    14041 cttttggtca caaatgcaaa atgccgtgga ctggcacacc gacatcgtca atggcctgat
    14101 caaaagcctg gtgtttggcg cggcagtgtc gctgattgcc gtgtttgaag gctttgacgc
    14161 caaacccacc gccgaagggg tgtcttcggc caccacgcgc accgtggtga cctcggcgct
    14221 gaccattctg gcgctggact ttgtattgac agctttcatg ttctaaccgc gaacaagact
    14281 gagaatttga gatgaaacgc gctacgattg atttatgggt gggcattttt gccgcgctgg
    14341 gctgcgccgc cgtgctgttt ctggcgctga aaaccgccaa tctgaccgtg ggcagcggga
    14401 gcaatggcac ctattcgctg tacgccgagt tcgacaatat tggtgggctg aaagtcaaag
    14461 cgccggtgaa gatggccggc gtggtggtgg gacgcgtcac cgacatccgt ctggacaaca
    14521 aatcgtttcg cgcccgcgcc accctggaaa tggacaagca ataccaattc agccgcgatg
    14581 tatcggccga aatcctcacc tccggcttgc tgggcgagca atacatcggc ctgttgcaag
    14641 gcggtgatcc agacaacctg gcggctggcg acaccatcaa gctgacctcc tcggcgctgg
    14701 tgctggagca gctgattggc aaattcatgc tcaatgccgc tgaaaaaccg gccgcaccgg
    14761 ccgatagcca ttaagcgttg tcggttctga tctccggaca acgccacgaa tacacgataa
    14821 atacacggga taccgaaaaa tgaagaatct tctgggcaaa ttattgatct gctgtgcttt
    14881 tgtgtcgttt tcgctgcacg ccatggcgga aaccccggac gaactggtgc gcgatacctc
    14941 gcgccaggtg ctggacgtgc tcaagcgcga caatggcaag aacaccaaga acatccgcgc
    15001 tgaagtggaa gcgctggtgc tgcccaagtt cgacttcacc cgcatgaccg cactggcggt
    15061 aggccgtggc tggcgtgatg ccacgccaga ccagcaaaaa cagctgaccg accagttccg
    15121 catgctgctg gtgcgtactt attcgtccac catgacccgc ttcaagaacg cccagatcac
    15181 cgtgcaggcc agcgccccgg ccgctgatgc gcgtgaagtc accgtgcgct ccgatgtcga
    15241 gctgccggcc aacgctggcg acaagcgcag cgcccaggtg gactacacgc tgatcaaaac
    15301 ccccaccggc tggcgcgtct acaatgtcag catcgaaggc gccagcctgg tgaccatcta
    15361 tcgcaacaac ttcaacgaca tcctgcgtca gggtggcccg gacgcgctga ttcagtcgct
    15421 gaaggacaaa aacgacgcct tgtccggtgg caagcatgcc agcaactgac gatggcctga
    15481 ccctggcgct gtctggcccg gtcaccttcg cccacaccgc ccgcctggcg cgcgagctgc
    15541 gcccgcgcgc agcggccgcc cgcgtgctgg attggtctgc cgtcacccag gtggattctt
    15601 ccgccgtggc gctgattctc gaactggcgc gcgccgctgg ccacggccac accctgcgcc
    15661 agccgccggc tggcttgtcc agcctgatca ccctgtatgg agtggaagac cttgttcgcg
    15721 caccgtgacc cccgacgcgc tcgccgcgtc ctcccggctc tgttggccgt cagcctgctg
    15781 gcaggctgcg ccgccgccac cccacaaaac cgcttcgacc cctacgaagc tgccaaccgc
    15841 aaggttcacg cttttaacga cgtggttgac cgcgccgccc tgcaaccgct ggcgcgcggc
    15901 tatcgcgccg tgacgcccga gccggcgcga gcggcggtca gcaatgtgtt tggcaacctg
    15961 gaagacgtgg tcagtgtggt caatcacctg ctgcaaggcc agtttgcccg cgccagttcc
    16021 gacctgatgc gcgtcgcact gaattccacc ctgggcctgg gcgggctgat cgacatcgcc
    16081 accccggcgg gcctgccctc gtacaagact ggctttggcg aaaccctggg ccgctggggc
    16141 taccagaaca gcgcctatgt ggtgctgccg ctgctggggc cttccaccgt gcgcgacacg
    16201 gtaggccgcg ccgccgacat tgccagcaat ccgcgcacct atctgctgcc agaacccgcc
    16261 gacgccaacg tggccaccgg ggtgaatctg ctcagccagc gcgccaatgc actggaactg
    16321 accgatgccg ccgacgaagc cgcgctggac aaatattcgt tcacccgcga cgcctacctg
    16381 caataccgcc gcacccgtat cggcgcagcg gacagcccgg caatgaacga cgaaccttcc
    16441 atcgacgcgc tggtgccgcc agaagcgccg gctaaataag tgccgctcac aaaacgcact
    16501 tgccgtgccg cgctgcaagt ggccgacata cgcctatgtg ctggaaaacg gccaggtgca
    16561 catgcacggc ccggcgcgcg aactggcgga tgaccctcgc gtggtcgagg cgtatctggg
    16621 gctggggagc aaacatcagg cgatgttgag cagttgaagc ttttggtaaa cccatggcag
    16681 acggcccgat gatcgggccg tttttttgtg cgccaaagtg cggggattcc aaacgcagaa
    16741 cgctggcgcg ctgtggatga cgcactatgc gtggtgatgg cgcagcgagg tgactgataa
    16801 tggggtaatc gtgaagctaa atggggtaac gagcatactt aataccccaa tttacggtag
    16861 cattacccca ttctttcact tgcccagtat ttgtccatgc actctctctc gccagactac
    16921 cttgccaagc tgcgctttga cgcacagcag ttggcaacat tgcgcgcctt gggggaatgc
    16981 agaggcaagc agcagctttt tgtggtgcag tcgcccgagg tcttaagcga tctgcgacaa
    17041 gtggctgtgg tcgagtccac cgagtcgtca aaccgcctgg agggtgtggt ggtggcgcct
    17101 catcggctca agtcactggt gctcaagaat gccacgccgc aaagccgttc cgagcaggaa
    17161 gtggcaggtt accgcgatgc cttggggctg atccacgaaa gtggcgagca gatgccgttt
    17221 tcggagggca cggtcttgca gcttcacggc atgttgtacc gctacatgcc gcaatctggc
    17281 ggacattgga aggccacgaa caacgacatt gtcgagcgcc accccgatgg cagctcacgc
    17341 attcgctttc ggccagtcgc cgcacacctt acccctattg cggtggcaga cctgatcgcg
    17401 cgctaccgct ttgctttgga ccagcatttg gccgacccct tggtgctggc gccgctggcc
    17461 atcctcgatt ttctgtgcat ccaccccttc ccggatggca atggccgcat ggcgcggttg
    17521 ctgaccttgc agctgctcta tcacttcgac tatgccgtag gccgcttcat cagcctggag
    17581 cgcatctttg aagaatcgaa ggaaagctac tacgaaacgc tggaggtcag ctctcaaggt
    17641 tggcacgagg ctgcgcacaa catcgcgccg tggcttaatt atttttgggg ggcactgctg
    17701 cgcgcctaca aagaattcga agagcgcgta ggcgccattg agcacggacg aggcgccaaa
    17761 ggcgagcgca ttcgcgcaga gattctcaaa cgccaactgc ctttctctat ctcggacatc
    17821 gaagaagcct gccccggcat cagccgcgac atggtgcgag ttgtgttgcg cgctatgaaa
    17881 atggaggggt taatcacgcc aacaggcaaa gggcgtgcag cgaagtggtt acacatcgag
    17941 gctgaggaac aaacctaaat gcaacagctt ctgacacaac atcaaagtct gtactatgcg
    18001 tggcagctaa cccggcgcac ggcctcgcga aatcgatctg gtggcagatc gcttggtcag
    18061 catggctcaa gcagttcaat ggaacaagca agcgcgcagc gtctttgaga acaaaacaga
    18121 ctgccaaacc gtgaaagccc gtttgatggc ggccttggtg cagccaaacc agcgaggaga
    18181 tcaatagtgc aagcgaatat caaaggtctg gtagaccggc tggagctttc ccaggccaag
    18241 gccatgatgc ctttgtacga ggccatttcc aacgccgtcg atgctatatg aagcgcatca
    18301 agacggcttt agcaatcaca cgattcgcat tcggccgggt gcgttcgatg accttgctcc
    18361 tcagcgtaga gaaggctatt gcaagaatct cgcgtgcagc aacggctaca tcgaaattat
    18421 tttctatgac aagcttctga aggatgcagc g
//
LOCUS       DAOPYX010000019        18387 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_3175_length_18387_cov_8.678486, whole genome shotgun sequence.
ACCESSION   DAOPYX010000019 DAOPYX010000000
VERSION     DAOPYX010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 18387)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18387)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18387
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3175_length_18387_cov_8.678486"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1329)
                     /locus_tag="PKN55_01990"
     CDS             complement(<1..1329)
                     /locus_tag="PKN55_01990"
                     /inference="COORDINATES: protein motif:HMM:NF025056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HNX70046.1"
                     /translation="MRQIFISYSHQDKAWKNRVVKQLRVLSRAGLDIWDDRRIAAGDD
                     WHKQIQKAIERCDVAVLLISADFLTSPFILDQEVPLLLQRRQEQGIRVIPLIVRPCEW
                     QRIDWLKTIQARPTDGKELSGMKKHQWESALSKLAGEIADLLRAPPPGTLSSPEAEAP
                     STGQNAPIRTDLTHLPAGAAHFLGRDNELAALDAAWASAGKTALVELIAPGGTGKTAL
                     VKRWLEHVKTDNWRGASRVFAWSFYSQGSGDERNASEDLFLAEAIKFFGVAVETSLNP
                     ADKGRALAEAIVAHRCLLVLDGLEPLQHPPGPLAGELRAPGLKALLTHLATVGRPGLA
                     LLTSREALKDLDEWANSPSGAALRIDLGNLSDRDGAALLHAEGARKAGRMPIAADDAE
                     LLEASRQVKGHALTLSLLGKYLALAFAGDIRQFDQIDWAEADAETKGGHAF"
     gene            complement(1593..3005)
                     /locus_tag="PKN55_01995"
     CDS             complement(1593..3005)
                     /locus_tag="PKN55_01995"
                     /inference="COORDINATES: protein motif:HMM:NF013122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HNX70047.1"
                     /translation="MFDTPLAIVDLETTGGHITRDRITEIGILLVDGDQVRPWRALVN
                     PGMPIPPFIEQMTGISNAMVAEAPPFEALAAELLQLLHGRLFIAHNARFDYGFLKNAF
                     RRLELPWRAQQLCTVKLSRLLYPQHYKHNLDSLIARFNLGCANRHRALDDAEAVWRFL
                     GAARAELGDDAIAHAVRQLMGRPAALPPQLDANLIDELPDTPGVYRFWDAGGAPLYVG
                     KSINLRSRVLSHFSADTRINKEMRLSQQVARIDWIDTVGEFGALLVESRQIKTLQPVH
                     NVRGRRERELCAWQLYPREDGFFVPRLIRGDEVDLGEADSVFGVFRSAREAKKTLTDV
                     ADAYRLCHAVLGIDRVTGKKGQPCFARQLGRCHGACIGKESAEQHNQRLLAALARMQR
                     QPWPFAGAMAVVEEDPVSGSVERHVFDRWRFLGSWRDGEPVLEAEPGFELDTYKMLAG
                     CLRKLPKGARLEPWDGWPAA"
     gene            3170..4120
                     /locus_tag="PKN55_02000"
     CDS             3170..4120
                     /locus_tag="PKN55_02000"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018150326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HNX70048.1"
                     /translation="MTATLPAAFADRLSRILPADRLDGVLASFAAVKDVAFRINTLKA
                     QPDAVLAGLRAAGLNPRPVAWSPLAWVVDAAAKRALTETPAFYQGEIYIQNLASQLAP
                     LALAPQPGEMVLDLAAAPGGKTCQMAALMGNQGWLSAVEPVRDRYFRLKHNLDQQGVS
                     IAHCYQKDGRAVGALTPERFDRVLLDAPCSSEARFDLNDPDSMAHWSPGKVREVAHKQ
                     KRLLLSAIHALKPGGVLVYSTCSFAPEENECIVHAALHKLGDAVEVTPVDIPVLHQPG
                     LTEWDGKTLNPQLAHSVRVLPDSLVDGFYLARLVKQRSTQ"
     gene            complement(4211..4573)
                     /locus_tag="PKN55_02005"
     CDS             complement(4211..4573)
                     /locus_tag="PKN55_02005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HNX70049.1"
                     /translation="MTLHRHLPGARLAEAVVCNGIAWLAGQVPENLDADAQAQTANVL
                     AQIDTVLRDLGSDKSQVIDATIFLADLADYDAMNAAWDAWVIPGQTPARATVQAKLAN
                     PAWKVEIKIVAAVSASKA"
     gene            complement(4692..5885)
                     /locus_tag="PKN55_02010"
     CDS             complement(4692..5885)
                     /locus_tag="PKN55_02010"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018747043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HNX70050.1"
                     /translation="MEYLNGLIDLPWWGYVLVALGLTHITIASVTIFLHRHQAHRALD
                     LHPLPSHFFRFWLWLTTGMITKQWAAIHRKHHARCETPEDPHSPQVLGLKKVLWQGAE
                     LYRSACKDQSIMDKFGHGTPDDWLENNIYTPRNGQGIFLMLAIDLILFGPAGLAIWAV
                     QMVWIPFWAAGVVNGIGHYWGYRNFENEDAATNLVPWGILIGGEELHNNHHTFGTSAK
                     LSYKWYEFDIGWMYIRMLEIVGLAKVRRVAPHLALGEGQPAAPLAADTLQTIITNRYA
                     VAAQYARQLKSEYASEIERLLKSAKLPDFHGIHLPRKMKIWLKQDAKDTPECDRVALD
                     TLLAHSDKLHTIYTMRQELTRLWERSSRSRDELLHDLQDWCARAEASGIRALREFSLG
                     LRAAS"
     gene            6114..6737
                     /locus_tag="PKN55_02015"
     CDS             6114..6737
                     /locus_tag="PKN55_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012697404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HNX70051.1"
                     /translation="MDAVKIFIALLVLVNPIGAIPIFIGLTPDASAQERKRIAKTASI
                     AVAVIILTFVALGDSLIRLLGISMGSFQVGGGILVMMISLSMMNAAPTATKTTRQETE
                     EAGGRANIAVVPLALPLLTGPGTISTVIIYSGHAHGWWAYLSLVICALLIALACYVAM
                     IAATPVSRMLGQTGINIVNRVMGMLLAALSVEIIADGAVKLFPALGH"
     gene            6932..8746
                     /gene="typA"
                     /locus_tag="PKN55_02020"
     CDS             6932..8746
                     /gene="typA"
                     /locus_tag="PKN55_02020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018151069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HNX70052.1"
                     /translation="MSRNLRNIAIIAHVDHGKTTLVDQLLRQSGTFRDNQQVDERVMD
                     SNDLEKERGITILAKNTAIEYEGTHINIVDTPGHADFGGEVERVLGMVDGVVLLVDAQ
                     EGPMPQTRFVTKKALALGLRPIVVINKIDRPGARPGWVVDQTFDLFDKLGATDEQLDF
                     PIVYASGLNGFAKLELDQESNDMRPLFDVVLAHVPTPPGDAEAPLQLQISALDYSTYT
                     GRLGVGRVLNGRIKPGQAVVVMNHEDQVAQGRINQVLGFKGLERVPVDQAEAGDIIII
                     SGLEDIGIGVTICDRENPVGLPMLSVDEPTLTMDFMVNTSPLAGTEGKFVTSRQIRDR
                     LNKELLTNVALRVEDTGDADLFRVSGRGELHLTILLENMRREGFELAVAKPRVVYKEI
                     NGEKCEPYENLTIDLEDGHQGSVMEEIGRRRGELTNMESDGQGRTRLEYHIPARGLIG
                     FQSDFMTMTRGTGLMSHVFDDYAPVKADLPGRHNGVLVSQENGDAVAYALWKLEDRGR
                     MFVSPGDKLYEGMIIGIHSRDNDLVVNPIKGKQLTNVRASGTDEAVRLTTPIKLTLES
                     AVEFIDDDELVEITPQSIRIRKRHLNEHDRKKAVRGLL"
     gene            complement(8963..10909)
                     /locus_tag="PKN55_02025"
     CDS             complement(8963..10909)
                     /locus_tag="PKN55_02025"
                     /EC_number="6.2.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014087929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030729 - acetoacetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate--CoA ligase"
                     /protein_id="HNX70053.1"
                     /translation="MHTPLWTPSPERVAGCAMTQFRARAAALAGRDLPDQAALYAWSL
                     AEPGAFWRLLWDEAGVIGEPGDVALAPGATMREARWFPEGRLNVAENLLRRRDDGEAL
                     VFCGEGQVERRLSWAELHQQVAQAAQALRQAGVGVGDRVAGMLPNMPEAIIAMLASAS
                     LGAVWSSCSPDFGVDGALDRFGQVAPKVWLVPDGYYYNGKRVAVADKVAAIAAGLPDA
                     PQVVVVNYTGDGEVAALAGVVSWAAWLAPHPGGDIEFARLPFNHPLYILFSSGTTGKP
                     KCIVHGAGGTLLQHLKEHRLHADLRAGDRFFYFTTCGWMMWNWLVSGLASEATLLLFD
                     GSPFAQDGQLLWDWFARERGTHFGTSAKYLDAAAKQGLAPARSHDLRALRAIFSTGSP
                     LVAEGFDYVYRDIKADVQLSSISGGTDIVSCFALGSPVLPVYRGELQCRGLGMAVDIW
                     NEAGQPVREEKGELVCTAPFPSMPIGFWNDPDGSKYHHAYFARFADVWCHGDFAELTA
                     HDGMVIYGRSDAVLNPGGVRIGTAEIYRQVERVEEVLESLACGQRHDGDERVLLFVKL
                     RPGVTLDDALRERIARQIRQGASPRHVPARIVQVADIPKTLSGKIVELAVKNVIHGEP
                     VNNLGALANPEALEHFRDRAELRS"
     gene            complement(11107..12021)
                     /locus_tag="PKN55_02030"
     CDS             complement(11107..12021)
                     /locus_tag="PKN55_02030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009117903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HNX70054.1"
                     /translation="MRYPSHITLVEVGPRDGLQNEATPVPTDVKLQLIQRLAAAGLTH
                     IEAGSFVSPKRVPQMADSADIVRALQPAASPLHYPVLTPNLQGFDAALAAGAREVAVF
                     AAASESFSQRNIQCSIADSLARFAPVLAAARQHGVAVRGYVSCVLGCPYEGDIAPQAV
                     ADVSLRLLEMGCTEISLGDTIGVGTPAGVRRLLDALLPQIPASQLAGHFHDTYGMAIA
                     NIVAAMECGLATFDSSVAGLGGCPYAQGASGNVATEDVVWLLNGLGVECGVSLEALLD
                     ASALISGFLGKPPASRVARALLAKRGSE"
     gene            complement(12051..14009)
                     /locus_tag="PKN55_02035"
     CDS             complement(12051..14009)
                     /locus_tag="PKN55_02035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011135314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl/propionyl/methylcrotonyl-CoA carboxylase
                     subunit alpha"
                     /protein_id="HNX70055.1"
                     /translation="MFRSLLIANRGEIACRIIRTARAMGIRTIAVYSDADASARFVRL
                     ADEAWRLGPAPARDSYLRAERILDIARASGAEAIHPGYGFLSENAEFAQACADAGVVF
                     VGPPPAAIAAMGSKSAAKQLMERAQVPLTPGYHGDEQDAGFLHQQADQIGYPVLIKAS
                     AGGGGKGMRIVERSADFPDALASCRREASASFGDDRVLIERYLQKPRHIEIQVFADQH
                     GEAVYLFERDCSVQRRHQKVLEEAPAPGMDAARRQEMGEAACAAARAVGYVGAGTVEF
                     IVEPDGRFYFMEMNTRLQVEHPVTEMITGQDLVAWQLRVAAGEPLPLRQDALRIHGHA
                     IEARIYAEDPDRDFLPATGQLIRLRPPTESASVRVDTGVQEGDAITPYYDPMIAKLIV
                     WGDDRQQALARLDQALADYRVLGLTTNIAFLRRLARHPAFAGADLDTGLIARHQAELL
                     PPASALPDADALVAAACWLLAGEAAATPRHGWRLNAECQRRLALQYGEHALSIGAHLH
                     ADGSWSLSLPDGAVGQARAQRNGSQLSIQLDARRLHADCLQHGDALQVYLPSGLTAFT
                     LPDPYAAEADIQHGDTHLKAPMPGRVVALLAPLGEAVEKGAPLMILEAMKMEHTLVAP
                     GPGHVAAYYFAVGEQVSEGSDLLDFVLP"
     gene            complement(14032..14832)
                     /locus_tag="PKN55_02040"
     CDS             complement(14032..14832)
                     /locus_tag="PKN55_02040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019924171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HNX70056.1"
                     /translation="MYFETLELERNGPIATVWMNRPDLHNAFNETLIAELTRAFVVLD
                     GDDSVRVVILAGRGPSFCAGADLAWMRRAADYSEEKNRADALALARMLKTLYRMQKPT
                     IARVHGAAFGGGTGLTAACDMAIAADSATFALSEVRLGLIPATIGPYVADAIGWRQAR
                     RYFLSAERIDAALARQLGLVHDISPAEQLDARIGKLVQELCKGAPAAQAEAKLLLADL
                     RAGSPLDDALLEKTAWHIARVRSQPEAREGLSAFLDKRPPGWLPAKKP"
     gene            complement(14849..16447)
                     /locus_tag="PKN55_02045"
     CDS             complement(14849..16447)
                     /locus_tag="PKN55_02045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011135316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HNX70057.1"
                     /translation="MPIIDSKLNPRAADFQANAAQMRTLVADLRAKIARNALGGGEGP
                     RSKHVARGKLLPRERIDLLLDPGAPFLELSQLAAWDMYNNDAPGAGMISGIGRVSGID
                     CMIVANDATVKGGTYYPMTVKKHLRAQDIAQENHLPCIYLVDSGGAFLPLQDEVFPDR
                     DHFGRIFYNQANLSAAGIPQIAVVMGSCTAGGAYVPAMADESIIVRNQGTIFLGGPPL
                     VRAATGEVVTAEELGGADVHTRISGVADHYALNDAHALGLARQIVSHLNRQRPPGPVR
                     RDSQPPRYAAEELYGVIPVDAKKPFDVREIIARLVDDSALDEFKQNYGTTLVCGFAHL
                     DGWPVGVIANNGILFSESALKGAHFVELCCQRNIPLVFLQNITGFMVGKKYENGGIAR
                     DGAKLVTAVACARVPKFTVLIGGSFGAGNYGMCGRAYSPRFLWMWPNSRISVMGGEQA
                     AGVLAQVKREQLGEAFSAEDEEKLKAPVREQYERQGHPYYASARLWDDGVIDPAQTRD
                     VLAMGLSAAMNAPAEPTRFGVFRM"
     gene            complement(16479..17651)
                     /locus_tag="PKN55_02050"
     CDS             complement(16479..17651)
                     /locus_tag="PKN55_02050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018151764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HNX70058.1"
                     /translation="MSYTSLHFALSEELDMLRDSVRHFAEQEIAPYAAEIDQSNQFPP
                     ALWRKLGEMGLLGITVEEEYGGANMGYLAHMIAMEEISRASASVALSYGAHSNLCVNQ
                     IRRNGTAAQKQKYLPKLISGEHVGALAMSEPNAGSDVVSMKLRADKRGDRYVLNGSKM
                     WITNGGDADTLVVYAKTDVNAGPRGITAFLIEKDFKGFSAGSKLDKLGMRGSNTYPLF
                     FDECEVPEENVLGQVGEGVKVLMSGLDYERAVLAAGPLGIMQACMDITVPYLKERQQF
                     GEPIGNFQLMQGKLADMYVSLSASRAYVYAVGQALDRGEGGRQVRKDAAGVILYAAER
                     ATQMALDTIQCLGGNGYINDYPAGRLLRDAKLYEIGAGTSEIRRWLIGRELMAEQG"
     gene            complement(17777..18172)
                     /locus_tag="PKN55_02055"
     CDS             complement(17777..18172)
                     /locus_tag="PKN55_02055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012695698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HNX70059.1"
                     /translation="MRRTYTITELSSEFGVTLRTIRFYEEQGMLCPARQGRSRVYAAR
                     DRVRLKLILRGKRLGLSLSEIAEIIDLYEVARDESSQALTLLNKLSTRKKQLIQQRED
                     IEQVMHDIEQLEQYCHSVLQPPGLRADCA"
BASE COUNT         3160 a         5870 c         6025 g         3332 t
ORIGIN      
        1 aaaagcatga ccgcccttgg tttccgcatc cgcctcggcc caatctatct ggtcaaattg
       61 acggatgtcg ccggcaaagg ccaaggctag atacttgccc agcaggctca aggtcaaggc
      121 atggcccttg acctgtcggc tggcttccaa aagctcggca tcatcggcgg caatgggcat
      181 tctgccggcc ttcctggccc cttcggcatg cagcagggcc gcgccgtcgc ggtcgctcag
      241 attgcccagg tcgatgcgta gcgccgcgcc gctcgggctg ttggcccatt cgtccaaatc
      301 tttcagcgcc tcccggctgg tcagcagcgc caggccaggg cggccaacag tagccagatg
      361 ggtgagcaag gccttaaggc ccggcgcgcg cagctcgccg gccagcgggc cgggcgggtg
      421 ttgcagcggc tccaggccgt ccagcaccag caaacagcgg tgggcgacaa tcgcttccgc
      481 cagcgcccgg cctttatcgg cggggttgag gctggtttcg acggcaacgc caaaaaattt
      541 gatggcctcg gcgagaaaaa gatcttccga ggcattgcgc tcgtcgccgc tgccctggct
      601 gtaaaaactc caggcaaaca cccggctggc gccacgccaa ttgtcagttt tgacatgctc
      661 caaccaacgc ttgaccagcg cggtcttgcc ggtgccgccg ggggcgatga gttcgaccag
      721 ggcggttttg ccagccgacg cccaggcagc gtcgagggcg gcgagttcgt tgtcacggcc
      781 gaggaagtgg gcagcgccag cggggaggtg ggtgaggtcg gtgcggatgg gggcgttctg
      841 gcccgtggag ggcgcttcgg cctctggcga agaaagcgtg ccgggcggcg gcgctcgcag
      901 caggtcggca atttcgccgg ccagcttgga cagcgccgat tcccattgat gctttttcat
      961 gcctgacaat tctttgccat cagttggtcg tgcctggata gtcttcagcc agtcgatgcg
     1021 ctgccactcg caagggcgga cgatcagcgg aatcacccgg atgccttgct cttgacgtcg
     1081 ttgcagcagc agcgggactt cctggtcgag gatgaatggc gaggtgagga aatcagccga
     1141 aatcagcaat accgccacat cgcagcgctc gatggctttt tgaatttgct tatgccagtc
     1201 atcgccagcg gcaatgcgcc ggtcatccca gatgtccagg ccggcgcggg aaagcacgcg
     1261 gagctgtttg acgacacggt tcttccaggc tttgtcctgg tggctatagc tgatgaatat
     1321 ttggcgcaca gcatggattc cggggtggtt tctcgatgcc cgccccggcg ttcggcttcg
     1381 cgcttgatcg catgagaaat ccggtcagga gggcggcggg gcgggtgatg cgagaaatta
     1441 tgccagaagt ctgcgccacg ggatggcggc gtggcgcttg ctgcgcgcgc gaggcatggc
     1501 ggtgtgtctg ggcatggcga tttgttcggt gttgttttgc gcatgccgcg tcacacgttc
     1561 agcacgccgt gcccctgcgc gcaggcgcgc tattacgccg ccggccagcc gtcccacggc
     1621 tccagccgtg cgcctttggg cagtttgcgc aggcagccgg ccagcatttt gtaggtgtcc
     1681 agttcaaagc ccggctcggc ttccagcacc ggctcgccgt cgcgccagct gcccaaaaag
     1741 cgccagcggt cgaacacatg gcgctcgacg ctgccgctga ccgggtcttc ttccaccacc
     1801 gccatggcgc cggcaaacgg ccagggttgg cgctgcatgc gcgccagcgc cgccagcagg
     1861 cgttggttgt gctgttcggc gctttccttg ccgatgcagg cgccgtggca gcggccaagc
     1921 tggcgggcaa agcagggttg gccttttttg ccggtcaccc ggtcaatgcc cagcacggcg
     1981 tggcacagcc ggtaggcgtc ggcgacgtcg gtcagggttt ttttggcttc gcgcgcgctg
     2041 cggaacacgc caaacacgct gtcggcctcg cccaggtcca cttcatcacc gcgaatcagc
     2101 cggggcacga aaaaaccatc ttcgcgcggg taaagctgcc aggcgcacag ctcgcgctcg
     2161 cggcggccgc gcacattgtg cacgggctgc aaggttttga tctggcgcga ctccaccaaa
     2221 agcgcgccaa actcgccgac ggtgtcgatc cagtcgatgc gcgccacttg ctggctgagg
     2281 cgcatttctt tgttgatgcg ggtgtcggcg ctgaaatgcg acagcacccg gctgcgcagg
     2341 ttgatgcttt tgcccacata cagcggcgcg ccgccggcgt cccaaaagcg gtaaacgccg
     2401 ggggtgtcgg gcagttcgtc gatcaggttg gcgtccagct gcggcggcag cgccgctggg
     2461 cggcccatca gctggcgcac ggcgtgggcg atggcgtcgt cgcccagctc ggcgcgcgcc
     2521 gcgcccaaaa agcgccacac tgcttcggcg tcgtccagcg cgcggtggcg gttggcgcag
     2581 cccaggttaa agcgggcaat caggctgtcc agattgtgct tgtagtgttg cgggtagagc
     2641 aggcgcgaca gcttgaccgt gcacagctgc tgggcgcgcc agggcagctc cagccggcga
     2701 aaggcgtttt ttaaaaagcc gtagtcaaag cgggcgttgt gggcgatgaa caggcggccg
     2761 tgcagcagtt gcagcagctc ggcggccagc gcctcgaagg gcggcgcctc ggccaccatg
     2821 gcgttgctga tgccggtcat ctgctcgata aacggcggaa tcggcatgcc gggattgacc
     2881 agcgcgcgcc aggggcgcac ctgatcgcca tccaccagca ggatgccgat ttcggtgatg
     2941 cggtcgcggg tgatgtggcc gccggtggtt tccaggtcca cgatggcaag gggagtgtca
     3001 aacacgcaag ttctctcaaa acgccggccg cccgatgcga ccggctggca ttgtgacagg
     3061 ccacaccggg aaagtcatag tgcagaggcg cagttttttc aggcggggga attcccgcag
     3121 gggcgcgctg tgattacaat ccgccccgga ttttgacgag aggcccacca tgaccgccac
     3181 cctgcccgcc gcctttgccg accgcctgag ccgcatcctg cctgccgacc gcctggacgg
     3241 tgtgctggcc agctttgctg cggtcaagga cgtggctttt cgcatcaaca ccctgaaagc
     3301 acagccggac gccgtgctgg ccggcctgcg cgccgccggg ctgaacccac ggccggtggc
     3361 gtggtcgccg ctggcctggg tggtggacgc cgccgccaag cgcgcgctga ccgaaacccc
     3421 ggcgttttat cagggcgaga tttacatcca gaacctggcc agtcagttgg cgccgctggc
     3481 cttggcccca cagccgggcg aaatggtgct ggacctggcc gccgcgcccg gcggcaaaac
     3541 ctgccagatg gcggcgctga tgggcaatca gggttggctg tctgctgtcg agccggtgcg
     3601 cgaccggtat tttcggctca agcacaatct ggaccaacag ggggtgagca tcgcccactg
     3661 ctaccagaaa gacggccgcg cggtgggcgc gttgacgccc gagcggtttg atcgggtgct
     3721 gctggacgcg ccgtgctcca gcgaggcgcg ctttgatctg aacgacccgg attcgatggc
     3781 gcactggagc cccggcaagg tgcgcgaggt ggcgcacaag caaaagcgcc tgctgctgtc
     3841 ggccattcat gcgctcaaac cgggcggcgt gctggtgtat tccacctgct cgtttgcccc
     3901 ggaagaaaac gaatgcattg tccatgccgc cctgcacaag ctgggcgacg cggtggaagt
     3961 caccccggtg gacatcccgg tgctgcacca gccagggctg accgaatggg acggcaagac
     4021 gcttaacccg cagctggcgc acagcgtgcg cgtgctgccg gatagcttgg tggatgggtt
     4081 ttatctggcg cggctggtca agcagcgctc gacgcagtaa gcgccgctga cacaattcag
     4141 ctcgcgcaat atgacagggg tcgccaccgc gccaggaagg tttggtttgc ttgcgagcgg
     4201 ggggcggcgc ttatgctttg ctggcgctca ctgccgccac aatcttgatt tccaccttcc
     4261 aggccgggtt ggccagcttg gcctgcacgg tggcgcgggc cggggtctgg ccggggatca
     4321 cccaggcgtc ccaggcggcg ttcatggcgt cgtagtcggc caggtcggcc agaaagatgg
     4381 tggcgtcgat cacctggctt ttgtcgctgc ccaggtcgcg cagcacggtg tcgatttgcg
     4441 ccagcacatt ggcggtttgc gcctgggcgt cggcgtcgag gttttccggc acctggccgg
     4501 ccagccaggc aatgccattg cacaccacgg cttcggccag acgggcgcca ggcaggtgac
     4561 gatgcagggt catgaggtca tccttaatca gggttgcagt acagaaatcg cccattcatg
     4621 gtgggcgggt tgagcaaaac agcccggcgg gccggtgggc gcgtcgggct gtgatggcgt
     4681 cagtcgcgtg cttagctggc ggcgcgcagg cccagcgaga actcgcgcag ggcgcggata
     4741 ccgctggctt cggcgcgggc gcaccagtct tgcaggtcgt gcaagagttc gtcacgcgaa
     4801 cggctggagc gctcccacag gcgggtgagc tcctggcgca tggtgtagat ggtgtgcagc
     4861 ttgtcgctgt gcgccagcag ggtgtccagc gccacacggt cgcattccgg ggtgtccttg
     4921 gcatcctgct tgagccagat tttcatcttg cgcggcaggt ggatgccgtg gaaatccggc
     4981 agcttggccg atttcagcag gcgttcgatt tcgctggcgt attcgctctt cagctggcgg
     5041 gcgtactggg cggccacggc gtagcggttg gtgatgatgg tctgcagggt gtcggcagcc
     5101 agcggggcgg ccggctggcc ctcacccagc gccagatgcg gcgccacgcg gcgcaccttg
     5161 gccaggccga cgatttccag catgcggata tacatccagc cgatgtcgaa ttcgtaccat
     5221 ttgtacgaca gcttggccga ggtgccaaag gtgtggtggt tgttgtgcag ctcttccccg
     5281 ccaatcagga tgccccaggg caccaggttg gtggcggcgt cttcgttttc aaagttgcgg
     5341 tagccccagt agtggccaat gccgttcacc acgccggccg cccagaacgg aatccacacc
     5401 atctgcactg cccagattgc caggccagcc gggccaaaca ggatcaggtc gatggccagc
     5461 atcaggaaaa taccctggcc gttgcgcggg gtgtagatgt tgttttccag ccagtcgtcc
     5521 ggcgtgccgt gaccaaattt atccatgatg gactggtctt tgcaggccga gcggtacagc
     5581 tcggcgcctt gccacagcac ttttttcagg ccgagcactt gcgggctgtg cgggtcttcc
     5641 ggggtttcgc agcgggcgtg gtgcttgcgg tggatggccg cccattgctt ggtgatcatg
     5701 ccggtggtca gccacaacca gaagcggaaa aaatggctgg gcagcgggtg caaatccagc
     5761 gcgcggtgcg cctggtggcg gtgcagaaag atggtgaccg aggcaatggt gatgtgggtc
     5821 aggcccaggg ccaccagcac ataaccccac cacggcagat caatcagccc gttcaggtat
     5881 tccatttgac ttcctttcgt cgaatgacgt aacaggtcat tttaccgcaa ttgccgcctg
     5941 tggcgtgcag ccggcaagat ttgccggaca aaatcggcta cactgccgcg ctcatcgcct
     6001 gtcccaacat ccacggaaca ggcgtgctac gctgaattca acccgcgcgc cccgccctat
     6061 ccctgctggc gcgttgcgcc ccatccgggc gcgctgccgt ggagccaatc acaatggatg
     6121 ccgtcaagat tttcattgcc ctgctggtgc tggtgaaccc gattggggcg attccgattt
     6181 ttatcggcct caccccggac gccagcgcgc aagagcgcaa gcgcatcgcc aaaaccgcct
     6241 cgattgccgt ggcggtgatc atcctgacct ttgtcgccct gggcgactca ctgattcgcc
     6301 tgctggggat ttccatgggc tcgtttcagg tgggcggcgg gattctggtg atgatgattt
     6361 cgctgtcgat gatgaacgcc gcgcccaccg ccaccaaaac cacccgccag gaaaccgaag
     6421 aagccggcgg ccgcgccaat attgccgtgg tgccgctggc gctgcccttg ctgaccgggc
     6481 cggggacgat ttccacggtg attatttatt ctggccacgc ccacggctgg tgggcgtatc
     6541 tgagcctggt gatctgcgcc ttgctgattg cgctggcctg ctatgtggcg atgattgccg
     6601 ccacgccggt gtcgcgcatg ctggggcaaa ccggcatcaa tatcgtcaac cgggtgatgg
     6661 gcatgctgct ggcggccctg tcggtggaaa tcatcgccga tggggcggtg aagctgtttc
     6721 cggcgctagg acattgagcc cccgctggat ggcgtaagcc gaccttggcg cacggcgggc
     6781 gcggcctgct gtgcggagtt ttgcccgccg ccctctgcgg cggttttgcc cccgctgtgt
     6841 tgcgacgcaa tgaatttttg tcgggccaag gtgacagcgc cttgcgccgt gctacaattg
     6901 cgcctatttt tttccacgca ttgaaccact tatgtcccgt aatctgcgca atatcgccat
     6961 catcgcccac gttgaccacg gtaaaaccac cctggttgac caactgcttc gccaatccgg
     7021 caccttccgt gacaaccaac aggttgacga acgggtgatg gactccaacg acctggaaaa
     7081 agagcgcggc attaccattc tggccaaaaa caccgccatc gaatacgaag gcacccacat
     7141 caatatcgtg gacaccccgg gtcacgctga ctttggcggt gaggtggagc gcgtgctggg
     7201 catggtggac ggcgtggtgc tgctggtgga cgcccaagaa ggcccgatgc cgcaaacccg
     7261 tttcgtcacc aaaaaagcgc tggccctggg cctgcgcccg atcgtggtga tcaacaagat
     7321 cgaccgtccg ggcgcgcgtc cgggctgggt ggtggaccaa accttcgacc tgttcgacaa
     7381 actgggcgcc accgacgagc agctggactt cccgattgtc tacgcctcgg gcctgaacgg
     7441 ctttgccaag ctggagctgg atcaagaatc caacgatatg cgcccgctgt ttgacgtggt
     7501 gctcgcacac gtgccgaccc cgccgggcga cgccgaagcc ccgctgcaac tgcaaatctc
     7561 cgcgctggac tactccacct acaccggccg cctgggcgtg ggccgcgtgc tgaacggccg
     7621 catcaagccg ggccaggccg tggtggtgat gaaccacgaa gaccaggtgg cgcaaggccg
     7681 catcaaccag gtgctgggct tcaagggcct ggagcgcgtg ccggtggacc aagccgaagc
     7741 cggcgacatc atcatcatct ccggcctgga agacatcggc attggcgtca ccatctgtga
     7801 ccgcgaaaac ccggtgggcc tgccgatgct gtcggtggac gaaccgaccc tgaccatgga
     7861 cttcatggtc aacaccagcc cgctggccgg caccgaaggc aagttcgtca ccagccgtca
     7921 gatccgcgac cgcctgaaca aagaactgtt gaccaacgtc gccctgcgcg tggaagacac
     7981 cggcgacgcc gatctgttcc gcgtgtccgg tcgtggcgaa ctgcacctga ccattctgct
     8041 ggaaaacatg cgccgcgagg gcttcgagct ggccgtggcc aagccgcgcg tggtgtacaa
     8101 ggaaatcaac ggcgagaagt gcgagccgta tgaaaacctg accatcgatc tggaagacgg
     8161 ccatcaaggt tcggtgatgg aagaaatcgg ccgccgccgt ggcgagctga ccaatatgga
     8221 atccgacggc cagggccgca cccgcctgga ataccacatc ccggcgcgcg gcctgatcgg
     8281 cttccagtcc gacttcatga ccatgacccg cggcaccggc ctgatgagcc acgtgtttga
     8341 cgactacgcc ccggtgaagg ccgacctgcc gggccgccac aatggcgtgc tggtgtcgca
     8401 agaaaacggc gacgccgtgg cctacgcgct gtggaagctg gaagaccgcg gccgcatgtt
     8461 cgtcagcccc ggcgacaagc tgtacgaagg catgatcatc ggcatccaca gccgcgacaa
     8521 cgacctggtg gtcaacccga tcaagggcaa gcagctgacc aacgtgcgcg cctccggcac
     8581 cgacgaagcc gtgcgcctga ccacgccgat caagctgacg ctggaatccg ccgtggaatt
     8641 catcgacgac gacgagctgg tggaaatcac cccgcaatcc atccgcatcc gcaagcgtca
     8701 cctgaacgaa cacgaccgca aaaaggccgt gcgcggcctg ctgtaagact ggctcgggcc
     8761 gcttggcccc acagtaaaaa cccccgctgg cgctttgcgt tggcgggggt ttttgtttgg
     8821 gcatgacgcc tgttacgctt ggtggggggt gagacgtact caccgaatgc agtaaaacag
     8881 ccctgactcg acatgcgcag accgtgccga tcgtcccggc aaatccaccc cgcagagctc
     8941 cacgggcatt ggcgcattgc gctcagctgc gcagttctgc ccgatcgcgg aagtgctcca
     9001 gcgcttccgg gttggccagc gcgcccagat tgttcaccgg ctcgccgtga atcacgtttt
     9061 tcaccgccag ttcgacaatc ttgccactga gggttttcgg gatatccgcc acctgcacga
     9121 tgcgcgccgg cacatggcgc gggctggcgc cttggcgaat ctgccgggca atgcgttcgc
     9181 gcagcgcgtc gtccagcgtg acgccggggc gcagtttgac aaacagcagc acccgctcgt
     9241 cgccgtcatg gcgctggccg caggccaggc tttccagcac ttcttccacc cgctcgactt
     9301 gccggtagat ttccgccgtg ccgatgcgca cgccgccggg gttgagcact gcgtccgagc
     9361 ggccgtagat caccatgccg tcgtgcgcgg tcagctcggc aaaatcgcca tggcaccaga
     9421 catcggcaaa gcgggcaaag taagcgtggt ggtatttgct gccgtccggg tcgttccaga
     9481 agccaatcgg catgctgggg aagggcgcgg tgcacaccag ctcgcctttt tcttcgcgca
     9541 ccggctggcc ggcttcgttc cagatgtcca ccgccatgcc cagcccccgg cattgcagct
     9601 cgccgcgata caccggcagc accgggctgc ccagggcaaa gcaggagaca atatcggtgc
     9661 cgccagaaat cgacgacagc tgcacatcgg ccttgatgtc gcggtagaca taatcaaagc
     9721 cttccgccac cagcggcgag ccggtggaga aaatcgcccg cagcgcgcgc aaatcatggc
     9781 tgcgcgccgg cgccagccct tgcttggcgg cggcgtccag gtatttggcc gaggtgccaa
     9841 agtgggtgcc gcgctcgcgg gcgaaccagt cccacagcag ttggccatcc tgggcaaacg
     9901 ggctgccgtc aaacagcaag agcgtggctt cgctggccag gccgctgacc agccagttcc
     9961 acatcatcca gccgcaggtg gtgaaataga aaaaccggtc gcccgcgcgc agatcggcgt
    10021 gcagccggtg ttctttcaga tgctgcaaca gcgtgccgcc cgcgccgtgg acaatgcact
    10081 tgggcttgcc ggtggtgccg ctggaaaaca ggatgtacag cgggtgattg aacggcaggc
    10141 gggcaaattc aatgtcgccg ccggggtgtg gcgccagcca ggcggcccag ctcaccacgc
    10201 cggccagcgc cgccacttcg ccgtcgccag tgtagttcac caccaccact tgcggcgcgt
    10261 ccggcagccc ggcggcaatc gccgccacct tatccgccac cgccacccgc ttgccgttgt
    10321 agtaataacc gtccggcacc agccacacct tgggcgccac ctggccaaag cggtccagcg
    10381 cgccatccac gccaaaatcc ggcgagcacg acgaccacac cgcacccagg ctggcgctgg
    10441 ccagcatggc gatgatggct tccggcatat tcggcaacat ccccgccacc cggtcgccca
    10501 cccccacgcc cgcctggcgc aaggcttgcg ccgcctgcgc cacctgctgg tgcagctcgg
    10561 cccagcttaa ccgccgctcg acctgccctt cgccacaaaa caccagcgcc tcgccgtcat
    10621 cccggcggcg cagcaggttc tcggccacat tcaagcgccc ctcaggaaac cagcgcgcct
    10681 cacgcatcgt tgcccccggc gccagggcga catcgcccgg ctcgccaatc accccggctt
    10741 cgtcccacag caaacgccag aatgcgcccg gctcggccag gctccacgcg tacaacgccg
    10801 cctgatccgg caaatcccgc ccggccaagg ccgctgcgcg cgcccgaaac tgggtcatcg
    10861 cgcagccggc aacccgctcc gggctgggcg tccacaaagg agtgtgcatg ctgaaatctc
    10921 cctgatcacc gcccggcgct gccggacggc gtgatgtctg tgcaaaatta agttattcgt
    10981 gtgactgtaa cggaaatatc tgcaaccatt tccgctctgt catgcccgcg cagccacggt
    11041 atagctgggt tcaagcctcc agcaaatagg attcggcgcg aagttctgcc ccagctcatc
    11101 ccatcgctat tcacttcccc gcttggccaa caaggcccgc gccacccgcg aggccggtgg
    11161 tttgcccaaa aagccgctga tcaaagcgct ggcgtccagc aaggcttcca gcgacactcc
    11221 gcattccacc cccaggccat tcagcaacca caccacatcc tcggtggcca cattgcccga
    11281 cgcgccttgc gcatacgggc agccgcccag cccggccacc gagctgtcga aggtcgccag
    11341 cccgcattcc atcgccgcga cgatattggc aatcgccatg ccgtacgtgt catgaaaatg
    11401 cccggccaac tggctggctg gaatctgcgg caacagcgcg tccagcagcc ggcgcacccc
    11461 ggctggcgtg ccgacgccga tggtgtcgcc cagcgagatt tccgtgcagc ccatctccag
    11521 cagacgcagg ctgacgtcgg ccaccgcctg cggggcgatg tcgccttcgt aggggcagcc
    11581 gagcacgcag gagacatagc cgcgcaccgc cacgccgtgc tgacgggcgg cggccagcac
    11641 cggggcaaag cgcgccaggc tgtcggcaat gctgcactgg atattgcgct gagaaaaact
    11701 ttcgctggcg gcggcaaaca ccgccacttc acgcgcgccg gcagccagcg cggcgtcaaa
    11761 gccttgcaga ttgggggtga gcaccgggta atgcagcggg ctggcggctg gctggagcgc
    11821 gcgcacgatg tcggcgctgt cggccatttg cggcacgcgc ttgggcgaga caaagctgcc
    11881 ggcttcgata tgcgtcaggc cagcggcggc caggcgctgg atcagttgca gcttgacgtc
    11941 ggtcggcacc ggcgtggctt cgttttgcaa gccgtcgcgc gggccgactt ccaccagggt
    12001 gatatgagag ggatagcgca tgtggggcgt cctgtggtga atcacggcgg tcagggcagc
    12061 acaaagtcca gcaggtcgct gccttcgctg acctgctcgc ccacggcaaa gtaatacgcc
    12121 gccacatggc ccggcccagg ggcgaccagg gtgtgctcca ttttcatggc ttccaggatc
    12181 atcagcggcg cgcctttttc caccgcctcg cccagcgggg cgagcagcgc caccacccgg
    12241 ccgggcatcg gcgctttcag gtgggtgtcg ccgtgctgga tgtcggcttc ggcggcgtac
    12301 gggtcgggca gggtgaaggc ggtcagcccg ctgggcagat acacctgcaa ggcgtcgccg
    12361 tgttgcaggc agtcggcgtg cagccggcgg gcgtccagct ggatgctgag ctggctgccg
    12421 ttgcgctggg cgcgcgcctg accgactgcg ccatcgggca agctcagcga ccagctgccg
    12481 tcggcgtgca ggtgcgcgcc gatgctcagg gcgtgttcgc cgtattgcag cgccaggcgg
    12541 cgctggcatt cggcgttcag ccgccagccg tggcgcgggg tggcggcggc ttcgccggcc
    12601 agcagccagc aggcggcggc caccagcgcg tcggcgtcgg gcagggcgct ggccggcggc
    12661 agcagctcgg cctggtggcg ggcaatcagg ccggtgtcca ggtcggcgcc ggcaaaggcc
    12721 ggatggcgcg ccagccggcg caaaaaggcg atattggtgg tcaggccgag cacgcggtaa
    12781 tcggccagcg cttggtcgag ccgcgccagc gcctgttggc ggtcgtcgcc ccagacaatc
    12841 agcttggcga tcatcgggtc gtagtagggg gtgatggcgt cgccttcctg cacgccggtg
    12901 tccacccgca cgctggcgct ttctgtgggc ggacgcaggc ggatcagctg gccggtggcc
    12961 ggcaaaaagt cgcggtccgg gtcttcggcg tagatgcgcg cctcgatggc gtggccgtgg
    13021 atgcgcagcg cgtcctggcg cagcggcagc ggctcaccag ccgccacacg cagctgccag
    13081 gccaccaggt cctggccggt gatcatttcg gtgaccgggt gctccacttg caggcgggtg
    13141 ttcatctcca tgaagtaaaa gcggccgtcg ggctcgacga tgaattccac cgtgccggcg
    13201 ccgacatagc ccaccgcgcg ggcggcggcg caggcggctt cgcccatttc ctgacggcgg
    13261 gcggcgtcca tgccgggggc gggggcttct tccagcactt tctggtggcg gcgctgcacc
    13321 gagcaatcgc gctcgaacaa atacactgct tcgccgtgct ggtcggcaaa cacctggatt
    13381 tcgatatggc gcggcttttg caaatagcgt tcgatcagca cgcggtcgtc gccaaagctg
    13441 gcgctggctt cgcgccggca cgaggccagt gcatcgggaa agtcggcgct gcgctcaaca
    13501 atgcgcatgc ctttgccgcc gccgccggcc gaggccttga tcagcaccgg gtagccaatc
    13561 tggtcggctt gctggtgcaa aaagccggcg tcttgttcgt cgccgtggta gccgggggtc
    13621 agcggcacct gggcgcgctc catcagctgt ttggcggcgg atttgctgcc catggcggcg
    13681 atggcggccg gcggcgggcc gacaaacacc acgccggcat cggcgcaggc ctgggcgaat
    13741 tcggcgtttt cggacagaaa gccataaccg gggtggatgg cctctgcgcc gctggcgcgg
    13801 gcgatgtcca gaatccgctc ggcgcgcaga tagctgtcgc gcgccggggc cgggcccagt
    13861 cgccaggctt cgtcggccag gcggacaaag cgcgcgctgg cgtcagcgtc ggaatacacg
    13921 gcaatggtgc ggatgcccat ggcgcgggcg gtgcggatga tccggcaggc gatttcgcca
    13981 cggttggcga tcagcagtga gcggaacata aatatccttc aatcgcgaag ttcagggctt
    14041 tttcgccggc aaccaacccg gcgggcgttt atcgagaaag gcgctcagcc cttcgcgcgc
    14101 ctcgggctgg ctgcgcacgc gggcgatgtg ccaggcggtt ttttccagca gggcgtcgtc
    14161 cagcggcgag ccggcgcgca ggtcggccag caagagcttg gcttcggcct gggcggcggg
    14221 cgcgcccttg cacagctctt gcaccagctt gccgatgcgg gcgtccagct gttcagccgg
    14281 gctgatgtcg tgcaccaggc ccagctggcg cgccagcgcg gcgtcgatgc gttcggccga
    14341 caaaaaatac cggcgcgcct gccgccagcc gatggcgtcg gcgacatagg ggccgatggt
    14401 ggccgggatc agccccagac gcacttcgga cagggcaaag gtggcgctgt cggcggcaat
    14461 cgccatatcg caggcggcgg tcagcccggt tccgccgcca aaggcggcgc cgtgcacgcg
    14521 ggcgatggtg ggtttttgca tgcggtacag ggttttcagc atgcgcgcca gcgccagcgc
    14581 gtcggcgcgg tttttttctt cgctgtaatc cgctgcgcgg cgcatccagg ccaggtcggc
    14641 cccggcgcag aaactggggc cgcgcccggc cagaatcacc acgcgcacgc tgtcgtcgcc
    14701 gtccagcacg acaaaggcgc gggtcagctc ggcaatcagc gtttcgttaa aggcgttgtg
    14761 caggtcgggg cggttcatcc acaccgtggc gatcgggccg ttgcgttcca gttccagggt
    14821 ttcgaagtac atggtggcgt ccttgtggct acatgcggaa cacgccaaag cgtgttggct
    14881 cagccggcgc attcatcgcg gcggacaggc ccatggccag cacgtcgcgg gtttgcgccg
    14941 ggtcaatgac gccatcgtcc cacaaacggg cgctggcata gtaggggtgg ccctgacgtt
    15001 cgtattgctc gcgcaccggg gctttgagtt tttcttcgtc ctcggcgctg aatgcctcgc
    15061 ccagttgctc gcgcttgacc tgggccagca cgccggccgc ctgttcgccg cccatcaccg
    15121 aaatgcggct gttgggccac atccacaaaa agcgcgggct gtaggcgcgg ccgcacatgc
    15181 cgtagttgcc ggcgccaaag ctgccgccaa tcagcacggt gaacttgggc acccgcgcgc
    15241 aggccacggc ggtgaccagc ttggcgccgt cgcgggcaat gccgccgttt tcgtattttt
    15301 tgccgaccat aaagccggtg atgttctgca agaacaccag cgggatattg cgctggcagc
    15361 acagctcgac aaagtgcgcg cccttgagcg ccgactcgga aaacaagatg ccattattgg
    15421 cgatcacacc caccggccag ccgtccagat gggcaaagcc gcacaccagg gtggtgccat
    15481 agttttgctt gaattcgtcc agcgcggagt catccaccag ccgggcgatg atttcgcgca
    15541 catcaaacgg ctttttggcg tctaccggaa tcacgccata cagctcttcg gcggcgtagc
    15601 gcggcggctg gctgtcgcgg cgcaccgggc ccggcgggcg ctggcggttg aggtggctga
    15661 caatctgccg ggccaggccc agcgcgtggg cgtcgttcag cgcgtagtgg tcggccacgc
    15721 cagagatgcg ggtgtgcaca tcggcgccgc ccagttcttc ggcggtcacc acttcgccgg
    15781 tggcggcgcg caccagcggc gggccaccca gaaaaatggt gccctgattg cgcacgatga
    15841 tggattcatc ggccatggcc ggcacatagg cgccgccagc agtgcaactg cccatcacca
    15901 cggcgatttg cggaatgccg gcggcgctga gattggcctg gttgtaaaag atgcggccaa
    15961 agtgatcccg gtcgggaaac acttcgtcct gcaagggcag aaaagcgccg ccggaatcca
    16021 ccagataaat acatggcagg tggttttcct gggcgatgtc ctgcgcgcgc aggtgttttt
    16081 tcaccgtcat cgggtaatag gtgccgccct tcaccgtggc gtcgttggcg acaatcatgc
    16141 agtcgatgcc ggacacccgg ccaatgccgc tgatcatgcc ggcgccaggg gcatcgttgt
    16201 tgtacatgtc ccaggcggcc agctgtgaca gttccagaaa cggcgcgcca gggtccagca
    16261 acaagtcgat gcgttcgcgt ggcagcagct tgccgcgcgc cacatgcttg ctgcgcgggc
    16321 cttcgccgcc gcccagcgca ttgcgggcga tttttgcgcg caggtcggcc accagcgtgc
    16381 gcatctgcgc ggcgttggcc tgaaaatccg ctgcgcgcgg gttgagtttg gaatcaataa
    16441 tcggcatctt ggcctccctt ggacgataag gatgtcgctc agccttgttc ggccatcagt
    16501 tcacggccaa tcagccaacg gcggatttcg ctggtgccgg cgccgatttc atacagcttg
    16561 gcgtcgcgca gcaggcgacc ggccgggtag tcgttgatat agccattgcc gcccaggcat
    16621 tggatggtgt ccagcgccat ctgggtggcg cgctcggcgg cgtacagaat caccccggcg
    16681 gcgtccttgc gcacttgccg gcccccttca ccgcgatcca gcgcctgacc caccgcgtac
    16741 acataggcgc ggctggccga caggctgacg tacatatcgg ccagcttgcc ctgcatcagc
    16801 tggaagttgc caatcggctc gccaaactgc tgacgctctt tcagataagg cacggtgatg
    16861 tccatgcacg cctgcatgat gcccagcggg ccggcggcca gcaccgcgcg ttcgtagtcc
    16921 agcccgctca tcagcacttt cacgccttcg cccacctggc ccagcacgtt ttcttccggc
    16981 acttcgcact cgtcaaaaaa cagcgggtag gtgttggagc cgcgcatgcc cagcttgtcc
    17041 agcttgctgc cggccgagaa gcccttgaag tctttttcga tcaagaacgc ggtgatgccg
    17101 cgcgggccgg cgttgacgtc ggtcttggcg tacaccacca gggtgtcggc gtcgccgcca
    17161 ttggtgatcc acatcttgct gccattgagc acatagcggt cgccgcgctt gtcggcgcgc
    17221 agtttcatgc tcaccacatc cgagccggcg ttgggttcag acatcgccag cgcgccgaca
    17281 tgctcgccag aaatcagctt gggcaggtat ttttgctttt gcgccgcggt gccgttgcgg
    17341 cgaatctggt tgacgcacag attggagtgc gcgccgtaag acagcgccac cgaggctgaa
    17401 gcgcggctga tttcttccat ggcgatcatg tgcgccaggt aacccatatt ggcgccgccg
    17461 tattcttctt ctacggtgat gcccagcagg cccatctcac ccagcttgcg ccacagcgcc
    17521 ggcggaaact ggttgctctg gtcgatttcg gcggcgtaag gggcgatctc ctgctcggca
    17581 aagtggcgca cgctgtcacg cagcatgtcc agttcttcac tcaaggcaaa atgcaggctg
    17641 gtgtacgaca tggtgtcaat ctcctcggtg gtgatccgcg cgccgtgcgg cgtggccggc
    17701 tgtgctgcgg gaatttatag tgtgggtaag tgtattacgc ttacgtaaac gtaaatcaag
    17761 cggcgagtga aaatccttat gcgcagtcgg cgcgcaggcc cggcggctgc aacacgctat
    17821 ggcagtattg ctccagttgc tcgatatcgt gcatcacctg ttcgatgtct tcacgttgct
    17881 gaatcagctg ttttttgcgc gtggacaatt tattcagcag ggtcagcgcc tgcgacgatt
    17941 catctcgcgc aacttcatat agatcaatga tttctgcgat ttccgacaaa gacaagccca
    18001 ggcgcttgcc gcgcaggatc aacttcagcc gcacccggtc gcgcgcggcg tacacccggc
    18061 tgcgcccttg gcgggccggg cagagcatgc cttgctcttc ataaaaacga atggtgcgca
    18121 aagtgacgcc aaactcgctg gataactcgg tgatggtgta agtgcgacgc atcgcggggc
    18181 tcgctgttgt gacgtttacg taagcgtaaa ctatgctttt gcatttggca agcgggtggc
    18241 gaggtgggcg tgtttgcgct ggtgcggtgg ggttggtgtg tcaatgaaat gctaacgtgg
    18301 tggcgtgttg gctgagtgag tggtttttta catatataag gaataaagct gaaatcgggg
    18361 tggcctgtcg tgctggcagt ctggaat
//
LOCUS       DAOPYX010000020        17998 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_3273_length_17998_cov_11.338126, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000020 DAOPYX010000000
VERSION     DAOPYX010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 17998)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17998)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17998
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3273_length_17998_cov_11.338126"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1142)
                     /locus_tag="PKN55_02060"
     CDS             complement(<1..1142)
                     /locus_tag="PKN55_02060"
                     /inference="COORDINATES: protein motif:HMM:NF024140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MCP four helix bundle domain-containing protein"
                     /protein_id="HNX70060.1"
                     /translation="MGRMTLSARLMLGIGMMVLSLLVTAALGIYGLTQSQHTMQKMVE
                     KDWERARAAADIIYMANQFNNGMFLMFDNPSLLPEVKAKFPGQRGRISENFKKLATLM
                     TEPEEKAFLDDLTAKRKAYTDVYFKTFKLFEEGKFDEGRLVFKNEAAGPLVTYMKKLE
                     DLVDEQNKHFDTNAKTAEDAADVLRTLLVVVTLGACVVAFATGFWIVRSVTVPLGGEP
                     HVASEVMRKIAKGDLSAHVPVKSGDRSSLLADLASMRDGLREVISSIQKNSLEVAAAA
                     QQVSVASSQVASRSMEQAESASSMALAVETLSTGISGVSENASTTKQIAHEASTRSHT
                     GREAISSTASEMEAIASLVQEAASGIHAMAEKSQQISGIVAVIREIA"
     gene            complement(1331..2092)
                     /locus_tag="PKN55_02065"
     CDS             complement(1331..2092)
                     /locus_tag="PKN55_02065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014085645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HNX70061.1"
                     /translation="MPRADVLLVSRGLAVSRTAAQHLIDTGRVYHGPANQRHRVGKPS
                     AKLDDDVELYVEPHADDRFVSRGGLKLAGALAEVGLDVLGWRALDVGMSTGGFTDCLL
                     QAGAARVVGIDVGHDQLHPRLRADDRVRWFEGVNARAPELDSLRQANDGALFDLIVGD
                     VSFISLTLTLPPLLALLRPGGHILHLVKPQFEVGREGIGRGGLVKDPSRYLDVRMRME
                     SMAVENHLNWLNWLDSPITGGDGNREFFMHAYKPL"
     gene            2311..2508
                     /locus_tag="PKN55_02070"
     CDS             2311..2508
                     /locus_tag="PKN55_02070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX70062.1"
                     /translation="MAKALEKSSEALEHATYFYASWACCLGMMYRKMAHSCNTFVIGQ
                     GKGVPSWLMYANTAAPAYWHV"
     gene            complement(2530..3024)
                     /locus_tag="PKN55_02075"
     CDS             complement(2530..3024)
                     /locus_tag="PKN55_02075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008954808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HNX70063.1"
                     /translation="MPLFTRRHALALAAASVCSLALPARAAPLDGVFYPDLAGKSQGL
                     TGAWRGKVVVLNFWATWCAPCREEMPMLNQYADRYGAAPLAVVGVAIDDKVAVRNFVQ
                     QFNIRYPVLLGDSSALNVMRAAGNQVGVLPFTLVLDKRGEIVARLTGKLSEAQLDAAV
                     RAHL"
     gene            3127..4065
                     /locus_tag="PKN55_02080"
     CDS             3127..4065
                     /locus_tag="PKN55_02080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011137824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HNX70064.1"
                     /translation="MSVLVCGSLAYDTLLHFDGAFSDHLLPDQLHKLSVCFKVPRLRR
                     EFGGCAGNIVYNLRALGGHPCLLASVGEDFAPYQAHLDALGVDSRLVRTVPGQYTAQC
                     FITSDQADNQITAFHPGAMDNPPAAHPSAAGPLQLAILAPGGPQSTLQLADELSALSV
                     PFMFDPGQELPLFSVEQLHALIARARWLALNDYESELLMQRTGLDVAQLAARVAGLVI
                     TRGGQGSDLYADGQHWRVPAAPADAIVDPTGCGDAYRAGLLHGLAQGWPWLDCGRLAS
                     VLGAIKIASRGGQNHLLNHDDIRQRYQAAFGEPCPL"
     gene            complement(4186..4722)
                     /gene="ruvC"
                     /locus_tag="PKN55_02085"
     CDS             complement(4186..4722)
                     /gene="ruvC"
                     /locus_tag="PKN55_02085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HNX70065.1"
                     /translation="MSAPRRVLGIDPGSRITGFGVVDLHGQHRQYVASGCIRAPEGAP
                     LPERIRAILDGIAEVTRLHQPTEAAVEQVFVNVNPASTLLLGQARGAALSALVLANLP
                     IAEYTALQVKQSVVGNGHAGKEQVQAMVVRLLKLNGTPQADAADALAVALCHANHSQG
                     ALSALQHSGLRLRRGRLG"
     gene            complement(4719..5414)
                     /locus_tag="PKN55_02090"
     CDS             complement(4719..5414)
                     /locus_tag="PKN55_02090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003783971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HNX70066.1"
                     /translation="MAAPACPAMVLAAGRGERMRPLTDTTPKPLLQVGAEPLIGWHLR
                     RLAAAGVRRVVVNHAHLGAQIEARLGDGAAWGVQLAYSAEPSALETAGGIATALPLLG
                     EGPFMVVNGDVYTDYPFARLLARAPQLDADGVLAHLVLVANPEHNPKGDFALDADGRV
                     HDAPGLTFSGIGVYHPALFADTPAHAPAKLAPLLRAAMARGQVSGECWDGQWLDVGTP
                     ARLDDARALAAGA"
     gene            complement(5414..6412)
                     /locus_tag="PKN55_02095"
     CDS             complement(5414..6412)
                     /locus_tag="PKN55_02095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012698633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HNX70067.1"
                     /translation="MTARFDTLSAWLATQRPGQTFTLKPASSDASFRRYFRATFADGA
                     TAIVMDAPPEHENCEPFVRVSGLLAAAGVHVPRVHAQDLQQGFLLLDDLGSTTYLAGL
                     TEDKARALYLDAIDALVLIQQAAGGDSLPPYDEALLTREMNLFPEWFASRHLGKPFDH
                     DTLTVWKDAQRLLLDAIQAQPRVLVHRDYHCRNLMVSQPNPGVLDFQDAVYGPLTYDL
                     VSLLKDAYLEWPEEFILDMVIRYWEKARKAGLPVQADIDSFYRDFEWMGVQRHLKVAG
                     IFARLSHRDGKHAYLDDIPLVVHYLKRTCKRYTELLPLGRLIQSLVGEDEQVGYTF"
     gene            6506..8758
                     /locus_tag="PKN55_02100"
     CDS             6506..8758
                     /locus_tag="PKN55_02100"
                     /inference="COORDINATES: protein motif:HMM:NF016348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS-assembly protein LptD"
                     /protein_id="HNX70068.1"
                     /translation="MPLFRLTPIAAALVVYCAHADPVEPADPVAGQIAVEADHMSGVG
                     SERVRAEGRVVAVRDDERVESDWLEYYQTRQHVRAGDRVHMTRGPDDLTGEALDYSME
                     ASQGTLENADVYRNQPGRRGAPGYRAGGALVRMTGKDTYEISQSRFTTCEPGRDDWYV
                     RAREMDLDYASNLGVARGAWVEFKGVPLLYTPWIDFPLQERRKSGLLAPTFKLGGGNG
                     IEFAQPYYLNLAPNYDATVIPRVLSRRGLMLGGEFRYLMPDYNGQMAGEIMPDDRKRN
                     GDTRFAYTLSHLQQAPSLNMAFGYNIRYASDNDYFRDFGDRRAIADGANLLREGWANW
                     HSPFGFFTVKAQRYKTMQDSKALVDEPYARLPQLTWNYGIGLPAGVEANFYAEYNQFS
                     HGQQVVNGGLNARQDGKRLTFYPSLSLPLQASYGFITPKVGLHYTEYWLSAQNAPVQG
                     ETGNYSRALPVASVDAGLVFERDSNWFGQSLTQTLEPRLYYLYIPYRDQSRLPNFDSA
                     RYDTSFAQLFNENHYAGGDRINNANQLTAAITSRFIDNDNGVERLRVSLGQRMYFDEQ
                     RVTLDANDIRRTKTLSDFLASVSGQLTRDAWMDTNYQYNRELGKAERYNLGLRYNPAP
                     GKTLSLRWRYERDTEVAGAALRGPSRQIDIAGQWPIARKWYVVGRQNYSFTGKQSLET
                     LLGVEYNDGCWAVRLVGQRYQKDLNTSTTAFYLQLELNGLGGIGSNPLQTLRQSIPGY
                     SKINDVPGKL"
     gene            8758..10035
                     /locus_tag="PKN55_02105"
     CDS             8758..10035
                     /locus_tag="PKN55_02105"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNX70069.1"
                     /translation="MKKLLIAALLATAFAQAGAAVREADRIVAVVNKDVITEVGLRQR
                     MAEAQAMLKKQNVPLPPAEVLRSQVFEQMVTESVQLQYAEQTGLRLDDAELERTLTRI
                     AESNKLSPEAFRERLKREGTDLNQFREQVRREVMLDRLREREVDNKVVVTDAEADLFM
                     KSAVNANRSEYHLAHILVSLPEQATPQVLEGRARRAEEALQKVRAGGNFAQLAASYSD
                     ARDAMSGGELGWRPASQLPAEFVRELDQLKAGGVTPILRSASGLHIFKLLDKRSGGQS
                     HVIEQIQARHILIKTNEAISEADAKRRILQIRDRLQSGMKFEEAARLYSEDASANRGG
                     DLGWMAQGDTVPEFERVMLALKPGVLSEPVRSPFGWHLIEVTGRRNQDVGAERDVQAV
                     KREIRARKAEQQYQDWIRQLRDSAHVENYLDER"
     gene            10039..11034
                     /gene="pdxA"
                     /locus_tag="PKN55_02110"
     CDS             10039..11034
                     /gene="pdxA"
                     /locus_tag="PKN55_02110"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003741992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HNX70070.1"
                     /translation="MATALPVIAITAGEPAGIGPDLLALLAARPALPAGARWVAIADR
                     HLLASRAAQLGVDAQWTDFQPDAPAPARGLEVLHVPLAAPAEAGVLNVANGRYVLNTL
                     DAAIDGCLAGQFAAMVTAPVHKGVINDAGVAFSGHTEYLAERTHTPRVVMMLTGGGMR
                     VALATTHLPLAQVAPSLTPALLDEVLRIVHADLRDKFGIAQPHILVAGLNPHAGEGGH
                     LGREELDVIIPALDALRAAGLRLSGPLPADTLFNPATLADADAVLAMYHDQGLPVLKY
                     ASFGHGINVTLGLPIVRTSVDHGTALSLAGTGQIDPGSLDAAMALAQQLASVRAA"
     gene            11263..15117
                     /locus_tag="PKN55_02115"
     CDS             11263..15117
                     /locus_tag="PKN55_02115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012698549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/FMN-binding oxidoreductase"
                     /protein_id="HNX70071.1"
                     /translation="MNAPTPRLREIPYNYTSFSDREIVIRLLGEDIWSLLNELRGERR
                     TGRSARMLFEVLGDIWVVRRNPYLQDDLLDNPSRLTLLVGAMRHRLNDIGQRRAGNER
                     VSQMLAAAHQAVDSFAAAFADMAALRKQIQQRFAGITRKDNVAFDGLARVAHVTDATD
                     WRVEYPFVVLYPDTEDEIAHMVKACIELGLTIIPRGGGTGYTGGAVPLSPKSAVINTE
                     KLDRHQGVEYIELPGLEGKHATIQCGAGVVTARVSEAASAAGVVFAVDPTSAEASCIG
                     GNVAMNAGGKKAVLWGTALDNLASWKMVDPDGNWMFVERIGHNYGKIHDVDVASFRVR
                     RFAADGKTLLSENTLNIPGPSFRKVGLGKDVTDKFLAGLPGVQKEGTDGIITSARFVL
                     HRMPKHTRTVCLEFFGTVAEATPAIVEITDYFKPGGGGVLAGVQLAGLEHLDWRYVRA
                     VGYATKAKSKGRPKMVLIADIVSDDEAAVGEAASHIVRLANARNGEGFIAVTPEARKK
                     FWLDRSRTAAISRHTNAFKINEDVVIPLPRLGDYSDGIERINIELSMANKLRLLDQLT
                     EFFHGRLPVDTLGSAVTADELLGDRREAALALIDAVRARWSWINSQMDAPFADYTARY
                     PDAPLEPSAAHLSPATVFHAMRDFALRVSWKRELLEDLDTLFSGKTDAPIRQAVANIH
                     QQVLRGRVFVALHMHAGDGNVHTNIPVNSDDYDMLQTAHKAVARIMELARGLNGVISG
                     EHGIGITKLEFLRDEEIAPFVAYKQQVDPKGHFNQGKLLPGADLRNAYTPSFELLGAE
                     SLILEQSDLGEISASVKDCLRCGKCKPVCSTHVPRANLLYSPRNKILGVGLLTEAFLY
                     EEQTRRGVSLKHFDELTDVADHCTVCHKCVNPCPVKIDFGDVSVAMRNFLRKAGKKKF
                     NPGTAAAMAFLNAKDPATIKAMRAGMMGLGYKAQRAGHQLFKRLGLIQEQTSQPPSTV
                     GKPAVKSQVIHFFNKPMPGNLPKKTSRALLDIEDPNIVPVIRDPQKASEGAEAVFYFP
                     GCGSERLFSQVGLATQAMLYHAGVQTVLPPGYLCCGYPQNATGNADKGQAITTENRVL
                     FHRVANTLNYLDIKTVIVSCGTCMDQLQKYAFGQIFPGCRILDIHEYLMEKGIKLDGV
                     QGVRYMYHDPCHTPMKTHAPLKVVNELMGSEVKLTDRCCGESGTLAATRPDVSTQVRF
                     RKEEDIRKVAAGLREQSPDSEVKILTSCPACLQGLSRYHDDANADADYIVVEMAKAVL
                     GDNWMADYVAKASHGGVERVLL"
     gene            15223..15645
                     /locus_tag="PKN55_02120"
     CDS             15223..15645
                     /locus_tag="PKN55_02120"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012698606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HNX70072.1"
                     /translation="MTSACELCAADGGELLYRDAKLRIVLVNDADYPGFCRVIWNAHV
                     REMTDLPAVDRYYLLNTVLQVEALLREVLRPDKINLASLGNVVPHLHWHVIPRFTDDR
                     HFPAPVWATPQREAATAHTLPHLADCLRERLATLHQHP"
     gene            15838..17487
                     /locus_tag="PKN55_02125"
     CDS             15838..17487
                     /locus_tag="PKN55_02125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX70073.1"
                     /translation="MEIDMHYQATYLAARLAGFDKPQATTIAHAAQYVDESDMSRLQD
                     KDAGFWIRDFKPHPTVQSTNELIRDTVNLWKWDSSTRTGWSEAYLRHLRRVWACFHFL
                     PGNYGPDAPFSYEGPTEARGWRYDDQCAEEFSMLCLTNSPLVANMVNDLLNHQDQPYL
                     PHLIGVRMHVLADTWAHTYFAGTPSWCVNEADNPVTRVFPDGSTAEIKWGPGGQGREE
                     FSPGTSLSYWGMPFLGHGRMGHLPDYPFMRYMYPAKWSGQPIFKNNPRDYLNGMGQMI
                     QAMRCVLTGQPFVINQYAPLSEDVTFKINALIQMLENTDASVRTRKWAEALDSWTFDG
                     QCFGAPPPFRADAWLDEYKRTALENQPGTDYYRFNQAAVRHVQLVGDVLRTDAAITIQ
                     ENPNCAVQRVQLASRSGRPVYIGPMSRSSTLLGGIKYCFPRAATSPISLQLVMVDGRQ
                     ALETGGLVKIITEESAVGPEDCLGDWRTSDSLYYYYDGYAPTRQSWLLEKADGSKGPI
                     RSGDAIRLRNQETTKAISCGREWLSTSSGTSADTEWVIHYL"
BASE COUNT         3458 a         5837 c         5551 g         3152 t
ORIGIN      
        1 gcgatttcac gaatcacggc gacaatgccg gaaatctgct gggatttctc ggccatggcg
       61 tgaatgccgg aggcggcttc ctgcaccagc gaggcaatgg cttccatttc gctggcggtg
      121 gacgaaatcg cttcgcggcc ggtgtgcgag cgggtgctgg cttcgtgggc aatctgtttg
      181 gtggtgctgg cgttttcgct gacgccggaa atgccggtag acagggtttc caccgccagg
      241 gccatcgacg aggccgactc ggcctgttcc atcgagcgcg aagccacttg cgacgaagcc
      301 accgacacct gctgggcggc ggcagcaact tccagcgaat ttttctggat actgctgatc
      361 acttcgcgca ggccgtcacg catcgacgcc aggtcagcca gcaggctgct gcggtcgcca
      421 gacttgaccg gcacatgggc ggacagatca cccttggcaa tcttgcgcat cacttcgctg
      481 gccacatgcg gttcaccacc caacggcacg gtgacgctgc gcacaatcca gaagcctgtg
      541 gcgaaggcca ccacgcatgc acccagggtg accaccacca acagagtacg cagcacatca
      601 gcagcgtctt ctgcggtttt ggcattggtg tcaaagtgct tgttttgctc gtccaccaag
      661 tcttcgagtt ttttcatata ggtcaccaac ggacctgccg cctcgttttt gaacaccagg
      721 cggccttcgt caaacttgcc ttcttcaaat agcttgaagg tcttgaaata gacatccgta
      781 taggctttac gcttggcagt cagatcatca agaaaggctt tttcttccgg ctcggtcatc
      841 agcgtggcca gtttcttgaa attttccgag atgcggccac gctgaccagg aaatttggct
      901 ttgacttctg gcagcagcga ggggttatca aacatcagga acatgccgtt attgaactgg
      961 ttggccatgt aaatgatgtc agccgctgcg cgggcgcgct cccagtcttt ttccaccatt
     1021 ttttgcatgg tgtgctggct ttgcgtcagg ccataaatcc ccagtgcggc tgtcaccaac
     1081 agcgatagca ccatcatgcc aatgccaagc atcagccggg cagagagagt catgcgtccc
     1141 atggtcatta atctccggta agtctcaaat agcgtgtgtg tttagcgcga atacgtagaa
     1201 atacgtagcg caaccatggg ttgcgcggtt tttgtcattt ttgtggctat tgtaacacca
     1261 acatagcaca tgttgcggcg cagcaaaaga gtgtttcttg cgtttgtcac aacaaaaaaa
     1321 tctattgcat ttacagaggc ttgtatgcat gcatgaaaaa ttcccggttg ccatctccac
     1381 cggtgatcgg gctatccagc caattcagcc aattcaaatg attttctacc gccatacttt
     1441 ccattcgcat acgaacatca agataacgcg atgggtcttt taccagccca ccacggccaa
     1501 tgccttcccg ccccacttcg aattggggtt tcactaaatg cagaatatgt ccgcctggcc
     1561 gcaacagggc aagcaaggga ggcagggtca gggtcagcga aataaacgag acatcgccga
     1621 caatcagatc aaacaacgcg ccgtcattgg cctggcgcag gctgtccagc tcgggagccc
     1681 gggcattgac gccttcgaac cagcgtacac ggtcatcggc gcgcaagcgc gggtgcaatt
     1741 ggtcgtggcc aacatcaatg ccgaccaccc gcgcagcgcc tgcctgcaac aggcaatcgg
     1801 taaagccgcc agtggacatc cccacgtcca gcgcccgcca gcccagcaca tccaggccca
     1861 cttcagccaa cgcgccggcc agcttcaagc caccgcgcga gacaaagcgg tcatcggcgt
     1921 gtggttcgac atacagttcg acatcgtcat ccagcttggc actgggcttg cctacccggt
     1981 ggcgctggtt tgccgggcca tgatagaccc gccctgtgtc aatcaaatgt tgcgcggcag
     2041 tgcgcgagac ggccagtcca cgactgacca gcaagacatc ggctcgaggc atgttgtgct
     2101 tgagtgagaa ttattctttt attatatccc aggcccgacc cgacaggctg cttagagcgg
     2161 ctaacaaaac tcagcgcaac gcgcctggcc aggcgcacga ccgcagacag tacggtaagc
     2221 aacaacgcca ggagggtttt gttagccaaa gccgcacatg ccagagccag aatgggcgca
     2281 gaaaacaggc agaacaccag ggtaggccat atggcaaaag ccctggaaaa atcaagcgaa
     2341 gcattggagc atgctacgta tttttacgca agttgggcat gctgtttggg aatgatgtac
     2401 cgaaaaatgg cacattcatg caacactttt gtgattggcc agggcaaggg cgtgccgtca
     2461 tggctgatgt atgccaatac ggcagcgcca gcgtattggc atgtgtgagc ccgttggtgg
     2521 aaagctcgct tacaggtggg cgcgtaccgc ggcgtccagt tgcgcctcgc tgagtttgcc
     2581 ggtcagccgg gcgacaatct cgccgcgctt gtccagcacc agggtgaacg gcagcacccc
     2641 cacctgattg ccggccgcac gcatcacgtt cagcgcgctg ctgtcgccca gcagcaccgg
     2701 atagcggata ttgaactgct gaacaaaatt gcgcaccgcc accttgtcgt caatcgctac
     2761 gcccaccact gccagcgggg cggcgccgta gcggtcggca tactggttga gcatcggcat
     2821 ttcctcacgg cacggcgcgc accaggtggc ccagaaattc aacaccacca ccttgccgcg
     2881 ccaggcgccg gtcagccctt gcgatttgcc ggccaagtca gggtaaaaca ccccatccag
     2941 cggggcggcg cgcgccggca gggccaggct gcacacactg gcggcagcca gcgccagggc
     3001 gtggcgacgg gtaaagagag gcatggaggg cttccttgtt gatctgatca tgacattctg
     3061 tgcacaagac gggcgaatgc tagactagtg gccattgcaa tcttgataat aaaggcctgc
     3121 ttcgccatgt ccgtgcttgt ttgcggttcg ctggcctacg acaccctgct gcattttgac
     3181 ggggcgttca gcgaccatct gctgcccgac cagctgcaca agctgtcggt gtgtttcaaa
     3241 gtgccgcgcc tgcgccgcga gtttggcggc tgcgccggca atattgtcta caatctgcgc
     3301 gcgctgggcg ggcatccctg cctgctggcc agcgtcggtg aggactttgc cccttaccag
     3361 gcgcatctgg acgcgctggg ggtggacagc cggctggtgc gcacagttcc cggccagtat
     3421 accgcgcagt gtttcatcac ctccgaccag gccgataacc agatcaccgc gtttcatccc
     3481 ggcgccatgg acaacccacc ggcggcgcac ccgtcggccg ccgggccgct gcaactggcc
     3541 atcctggccc cgggcgggcc gcagtccacc ttgcaactgg ccgatgagct gagcgcgctg
     3601 agtgtgccgt tcatgtttga tccgggccag gagctgccgc tgttcagcgt cgagcaattg
     3661 catgcgctga ttgcccgcgc ccgctggctg gcgctgaacg actacgaatc agagttgctg
     3721 atgcagcgca ccgggctgga cgtggcgcaa ctggccgcgc gcgtggccgg gctggtgatc
     3781 acccgtggcg ggcaaggctc ggatttgtac gccgacggcc agcattggcg cgtgccggca
     3841 gcgccggccg acgcgattgt cgaccccacc ggctgcggcg acgcctaccg cgccgggctg
     3901 ttgcacggcc tggcgcaagg ctggccctgg ctggactgcg gccggttggc cagcgtgctg
     3961 ggggcgatca agatcgccag ccgcggcggg cagaaccacc tgctgaatca cgacgatatt
     4021 cgccagcgtt atcaggcggc gtttggcgag ccatgtccgc tgtgagtgac tgagtaggaa
     4081 agcgcggatt gctccagcac aatcgcgccg gccaaacaca ccgcaaaatc aaacccctcg
     4141 acgcccggct ttgctggcgt cgaggggttt ttacattggc cgcgcttaac ccaatcgccc
     4201 gcgccgcagg cgcaagccgc tgtgttgcag cgcgctgagc gcgccctggc tgtggttggc
     4261 gtggcacagc gccaccgcca gcgcgtcggc ggcgtcggcc tgtggcgtgc cgttgagctt
     4321 gagcaggcgc accaccatgg cctgcacttg ctctttgccg gcgtggccgt tgccgaccac
     4381 cgattgcttg acttgcagcg cggtgtattc ggcaatcggc agattggcca gcaccagcgc
     4441 cgacagcgcc gcgccgcgcg cctggcccag cagcagggtc gaggccgggt tgacgttgac
     4501 gaacacttgc tcgacggcgg cttcagtcgg ttggtgcagg cgggtgactt cggcaatgcc
     4561 gtccagaatg gcgcggatgc gctcgggcag cggcgcgcct tctggcgcgc ggatgcagcc
     4621 tgaggccaca tactggcgat gctggccgtg caaatccacc acgccaaagc cggtgatgcg
     4681 cgaaccaggg tcgatgccca gcacccggcg cggcgcgctc acgcgccggc cgccagcgcg
     4741 cgggcgtcgt ccagccgcgc cggcgtgccc acgtccagcc actggccgtc ccagcattcg
     4801 ccgctgacct ggccgcgcgc catggccgcg cgcagcagcg gggccagttt ggccggcgca
     4861 tgggccgggg tgtcggcaaa cagggccggg tgatacacgc cgatgccgga gaaagtcagc
     4921 cccggcgcat cgtgcacgcg gccgtcggcg tccagggcaa aatcgccctt ggggttgtgc
     4981 tcggggttgg ccaccagcac caggtgcgcc agcacgccgt cggcgtccag ctgcggcgcg
     5041 cgcgccagca ggcgggcaaa tgggtagtcg gtgtagacgt cgccattcac caccataaat
     5101 ggcccctcac ccagcaaggg cagcgcggtg gcgatgccgc cagcggtttc cagcgcgctg
     5161 ggctcggcgg aatacgccag ttgcacgccc caggcggcgc cgtcgcccag ccgggcttcg
     5221 atctgcgcgc ccaggtgggc gtggttgacc accacccgac gcacgccagc ggcggccagc
     5281 cggcgcagat gccagccaat cagcggctcg gcgcccactt gcagcagggg tttgggcgtg
     5341 gtgtcggtca gcgggcgcat gcgctcgccg cgtccggcgg ccagcaccat ggccgggcag
     5401 gcgggcgcgg ccatcagaag gtgtagccca cttgttcgtc ttcgcccacc agcgactgaa
     5461 tcaggcggcc cagcggcagc agctcggtgt agcgcttgca ggtgcgcttg aggtagtgca
     5521 ccaccagcgg gatgtcgtcc agataggcgt gcttgccgtc gcggtgcgac aggcgggcaa
     5581 agatgccggc cactttcagg tggcgctgca cgcccatcca ttcaaagtcg cgatagaacg
     5641 aatcaatgtc cgcctgcacc ggcaggccgg ccttgcgggc tttttcccag tagcggatga
     5701 ccatgtcgag gatgaattct tccggccatt ccagataggc gtctttcagc agcgacacca
     5761 gatcgtaggt gagcgggccg tacaccgcgt cctgaaaatc cagcacgccg gggttgggtt
     5821 ggctcaccat caggttgcgg cagtggtagt cgcggtgcac cagcacgcgc ggctgcgcct
     5881 gaatggcgtc cagcagcagg cgctgggcgt ctttccacac cgtcagggtg tcgtggtcaa
     5941 acggcttgcc cagatgacgg ctggcgaacc actcgggaaa caaattcatt tcgcgggtca
     6001 gcagcgcctc gtcgtagggc ggcaggctgt cgccgccggc tgcttgctgg atcagcacca
     6061 gcgcgtcgat ggcgtccagg tacagcgcgc gggccttgtc ttcggtgagg ccggccagat
     6121 aggtggtcga gcccagatcg tccagcagca aaaagccctg ttgcagatcc tgggcgtgca
     6181 cgcgcggcac atgcacgccg gcggcggcca gcaggccgga cacgcgcaca aagggctcgc
     6241 agttttcgtg ctcgggcggc gcatccatga cgatggcggt ggcgccatcg gcaaaggtgg
     6301 cgcggaagta gcgccggaag ctggcgtcgc tggaagccgg cttgagggtg aaggtttggc
     6361 cgggacgttg tgtcgccagc caggcggaaa gggtgtcaaa acgtgcagtc attgggggcg
     6421 ccagaattgc ggtaaacgct aaagtatgcg attttatacc gccttgattt ccaacgtaca
     6481 cgggcgcttg tgccccggat gttccatgcc gctctttcgc ctgaccccga ttgccgccgc
     6541 cctggtggtg tactgcgccc acgcagaccc cgtagaaccc gccgacccgg tcgccgggca
     6601 gattgccgtt gaagccgacc acatgagcgg ggtaggctcc gaacgcgtgc gcgccgaagg
     6661 ccgagtggtg gccgtgcgcg acgacgaacg ggtggagtcc gactggctgg aatactacca
     6721 aacccgccag catgtgcgtg ccggcgaccg tgtgcacatg acgcgcggcc cggacgacct
     6781 cactggcgag gcgctggatt acagcatgga agcctcgcaa ggcacgctgg aaaacgccga
     6841 tgtctaccgc aaccagcctg gccgacgcgg tgcgcctggc tatcgcgccg gtggcgcact
     6901 ggtgcgcatg accggcaagg atacctacga aatcagccaa tcgcgcttta ccacctgtga
     6961 gccggggcgc gatgactggt atgtgcgcgc ccgcgagatg gatctggact acgccagcaa
     7021 tctgggcgtg gcgcgtggcg cctgggtgga gttcaaaggc gtgccgctgc tgtacacgcc
     7081 atggattgat tttccgctgc aagagcgccg caaaagcggc ctgctggcgc ctactttcaa
     7141 gctgggcggc ggcaatggca ttgagtttgc ccagccgtat tacctgaatc tggcgcccaa
     7201 ttacgacgcc accgtcatcc cgcgcgtcct gtcgcggcgc ggcctgatgc tgggcggcga
     7261 gttccgctac ctgatgccgg actacaacgg gcagatggcc ggcgaaatca tgccggacga
     7321 ccgcaagcgc aatggcgaca cccgctttgc ctatacgctc agccaccttc agcaagcgcc
     7381 cagcctgaat atggcgtttg gctacaatat ccgctacgcc tcggataatg actatttccg
     7441 cgactttggc gaccgccgcg ccattgccga cggcgccaac ctgctgcgcg aaggctgggc
     7501 caactggcat agcccgtttg gctttttcac cgtcaaggcc cagcgctaca aaaccatgca
     7561 ggacagcaag gcgctggtgg atgagccgta tgcccgtctg ccgcagctga cctggaatta
     7621 cggcatcggc ttgccggctg gcgtggaagc caatttttac gctgaataca accagttcag
     7681 ccacggccag caagtggtca atggcggcct gaacgctcgc caggatggca agcggctgac
     7741 gttctatccg agcttgtcct tgcccttgca agccagctac ggctttatca ccccgaaagt
     7801 cggcctgcac tacaccgaat actggctgtc ggcgcaaaac gccccggtgc aaggcgaaac
     7861 cggcaattat tcgcgggcgc tgccagtggc cagcgtggac gccggcctgg tgttcgagcg
     7921 cgacagcaac tggtttggcc agagcctgac gcaaacgctg gaaccgcggc tgtactatct
     7981 gtacatccct taccgcgatc aatcgcgcct gcccaacttc gacagcgcgc gctacgacac
     8041 cagctttgcc cagctgttca acgaaaacca ctatgccggc ggcgaccgca tcaataacgc
     8101 caaccagctg actgccgcca tcaccagccg ctttatcgac aacgacaatg gcgtcgagcg
     8161 cctgcgcgtg tccttgggcc agcgcatgta ttttgacgaa cagcgcgtca ccctggacgc
     8221 caacgacatt cgtcgcacca aaaccctgtc cgattttctg gcctcggtca gcggccagct
     8281 gacccgcgac gcctggatgg acaccaacta ccagtacaac cgcgagctgg gcaaggccga
     8341 gcgttacaat ctgggcctgc gctacaaccc ggcgccgggc aagacgctga gcctgcgctg
     8401 gcgctacgag cgcgataccg aagtggccgg cgccgccctg cgtggcccgt cgcgacagat
     8461 cgacattgcc ggccagtggc cgattgcccg caaatggtat gtggtgggcc ggcagaatta
     8521 ttcttttact ggcaagcaat cgctggaaac cctgctgggc gtggaataca acgacggctg
     8581 ctgggccgtg cgcctggtgg gccagcgtta tcagaaagac ctgaacacca gcaccactgc
     8641 cttctacctg caactggagc tcaacggcct gggcggcatt ggcagcaatc cgctgcaaac
     8701 cctgcgccag agcatccctg gttatagcaa gatcaacgac gttcccggaa aactctgatg
     8761 aaaaaactgc tgattgccgc cctgcttgcc accgccttcg cccaggccgg cgccgccgtg
     8821 cgcgaggccg accgcattgt ggcggttgtc aacaaggacg tgatcaccga agtcggcctg
     8881 cgccagcgca tggccgaagc ccaggccatg ctgaaaaagc aaaatgtgcc gctgccgccg
     8941 gccgaggtgc tgcgctcgca ggtgtttgag caaatggtca ccgaatcggt gcaactgcaa
     9001 tacgccgagc agaccgggct gcgcctggac gacgccgagc tggagcgcac actgacccgc
     9061 attgccgaaa gcaacaagct cagcccggag gctttccgtg agcgcctcaa gcgcgaaggc
     9121 accgacctga accagttccg cgaacaagtg cgccgcgaag tgatgctgga ccgcctgcgc
     9181 gaacgcgaag tggacaataa agtcgtggtc accgacgccg aggcggatct gttcatgaaa
     9241 agcgcggtaa acgccaaccg cagcgaatac cacctggcgc acattctggt gtcgctgccc
     9301 gagcaagcca ccccgcaagt gctggaaggc cgcgcccgcc gtgccgaaga agcgctgcaa
     9361 aaagtgcgcg ctggcggcaa ctttgcccaa ctggcggcca gttattctga cgcgcgcgac
     9421 gccatgagcg gcggcgagct gggctggcgc ccggccagcc agctgccggc ggagtttgtg
     9481 cgcgagctgg atcagctcaa ggccggcggt gtcaccccca tcctgcgctc ggcctctggc
     9541 ctgcatatct tcaagctgct ggacaagcgc tccggcggtc agtcgcatgt gattgaacaa
     9601 atccaggccc gtcacatcct gatcaagacc aacgaagcca tcagcgaagc cgacgccaag
     9661 cgtcgtatcc tgcaaatccg tgaccgcctg caaagcggca tgaaatttga agaagccgcc
     9721 cgcctgtact ccgaagacgc cagcgccaac cggggcggcg acctgggctg gatggcgcag
     9781 ggcgacaccg tgccggaatt cgaacgggtg atgctggccc tcaagccggg cgtgctgtcc
     9841 gagccggtgc gctcgccgtt tggctggcac ctgattgaag tcactggccg ccgcaaccag
     9901 gacgtgggcg ccgagcgcga cgtgcaggcg gtcaagcgtg aaatccgcgc gcgcaaggcc
     9961 gagcagcaat accaggactg gattcgccag ctgcgcgaca gcgcccatgt ggaaaactac
    10021 ctggacgagc gttaagccat ggccacagcc ctgccggtga tcgccatcac cgccggcgag
    10081 cccgccggca tcggccctga tttgctggcc ttgctggccg ctcgcccggc gctgcccgct
    10141 ggcgcgcgct gggtggcgat tgccgaccgc catctgttgg ccagccgcgc cgcgcaactg
    10201 ggcgtggatg cccaatggac ggattttcag ccagacgcgc ccgccccggc gcgcggcctg
    10261 gaggtgctgc acgtgccgct cgccgcgccg gccgaggccg gcgtgctcaa cgtcgccaac
    10321 ggccgctatg tgctcaacac gctcgacgcc gcgattgacg gctgtctggc cgggcaattt
    10381 gccgccatgg tcaccgcccc ggtgcacaag ggcgtgatca acgacgcggg tgtggcgttt
    10441 tccgggcaca ccgaatacct ggccgagcgc acccacaccc cgcgcgtggt gatgatgctc
    10501 accggcggcg gcatgcgcgt ggcgctggcc accacccatc tgccgctggc gcaggtggcg
    10561 cccagcctga cgccggcctt gctggacgaa gtgttgcgca ttgtccacgc tgacctgcgc
    10621 gataaattcg gcatcgccca gccgcatatt ctggtggccg ggctgaatcc acacgcgggc
    10681 gaaggcggcc atctgggccg cgaagagctg gacgtgatca tcccggcgct ggacgccctg
    10741 cgcgcagcag gcctgcgcct gagcggcccc ttgccggccg acacgctgtt taacccggcc
    10801 accctggccg acgccgacgc cgtgctggcc atgtaccacg accaaggctt gccagtgctg
    10861 aaatacgcca gctttggcca tggtattaat gtcaccctgg gcctgccgat tgtgcgcacc
    10921 tcggtggacc acggcaccgc gctgagcctg gccggcaccg gccagatcga ccctggcagc
    10981 ctggacgccg ccatggcgct ggcgcagcag ctggccagcg tgcgcgccgc ctgagcggcg
    11041 cacacaaaac cccgcgccgc gtgcctgcgt caggcgcgcg gccgccccgc gcaacaccgc
    11101 ccggagggtt ttgtaagcgg cgctcacccc gcaaatcctg ttctttttcc gctgacttgg
    11161 gtcggcggcg gaataacctt gtttccaaac gtgtttttcg cagcgcgcca attccgctaa
    11221 aatgaacgac ttttccgtat ttggccgctg ccgcgagtcc cgatgaacgc tcccacaccc
    11281 cgcttgcgcg aaattccgta caactacacc tcgttttccg accgcgagat cgtcatccgc
    11341 ctgttgggcg aggacatctg gagcttgctc aacgagctgc gcggcgaacg ccgcactggc
    11401 cgttccgcgc gcatgctgtt tgaggtgctg ggcgatatct gggtggtgcg ccgcaacccc
    11461 tacctgcaag acgacctgct ggacaacccc agccggctga ccctgctggt gggcgccatg
    11521 cgccaccggc tgaacgacat cggccagcgc cgcgccggca acgagcgcgt cagccagatg
    11581 ctcgccgccg cccatcaggc ggtggacagc tttgccgccg cctttgccga catggccgcg
    11641 ctgcgcaaac agatccagca gcgctttgcc ggcatcaccc gcaaagacaa cgtcgccttt
    11701 gatggcctgg cccgcgtggc ccacgtcacc gacgccaccg actggcgggt ggaatacccg
    11761 tttgtggtgc tctacccgga caccgaagac gaaatcgccc acatggtcaa agcctgtatc
    11821 gaactgggcc tgaccatcat cccgcgcggg ggcggcactg gctacactgg cggcgccgtg
    11881 ccgctgtcgc ccaagtcggc ggtgatcaac accgaaaaac tcgaccgcca tcagggcgtg
    11941 gaatacatcg agctgcctgg cctggaaggc aagcacgcca ccatccagtg cggcgcgggc
    12001 gtggtcaccg cgcgcgtctc tgaagccgcc agcgcggcgg gcgtggtgtt cgccgtcgat
    12061 ccgacctcgg cggaagcctc ctgtatcggc ggcaatgtgg cgatgaacgc cggcggcaaa
    12121 aaagccgtgc tgtggggcac cgcgctggac aatctggcca gctggaaaat ggtcgacccg
    12181 gacggcaact ggatgtttgt cgaacgcatc ggccacaact acggcaagat tcacgatgtg
    12241 gacgtggcca gcttccgggt gcgccgcttt gccgcagacg gcaaaaccct gctgtcggaa
    12301 aacaccctga acatccccgg cccgtccttc cgcaaggtgg gcctgggcaa ggacgtgacg
    12361 gataaattcc tggccggcct gccgggcgtg caaaaagaag gcaccgacgg catcatcacc
    12421 agcgcgcgct ttgtgctgca ccgcatgccc aagcacaccc gcaccgtgtg tctggaattc
    12481 tttggcacgg tggccgaagc cacgccagcg attgtggaaa tcaccgatta cttcaagccc
    12541 ggcggcggcg gcgtgctggc tggcgtgcag ctggctggcc tggagcatct ggactggcgc
    12601 tatgtgcgcg cggtgggcta cgccaccaag gccaaatcca agggccggcc caagatggtg
    12661 ctgattgccg atattgtctc ggacgacgaa gccgctgtcg gcgaagccgc cagccatatc
    12721 gtgcgcctgg ccaatgcccg caatggcgaa ggctttatcg ccgtcacccc ggaagcgcgc
    12781 aagaaattct ggctggaccg cagccgcacg gcggcgattt cgcgccacac caacgccttc
    12841 aagatcaacg aagacgtggt gattccgctg ccgcgcctgg gtgattactc cgacggcatc
    12901 gagcgcatca atatcgagct gtcgatggcc aacaagctgc gcctgctcga ccaactgacc
    12961 gaatttttcc acggccgcct gccggtggac accctgggca gcgcggtcac cgctgatgag
    13021 ctgctgggcg accgccgcga agcggcgctg gcgctgatcg acgcggtgcg tgcgcgctgg
    13081 agctggatca acagccagat ggacgcgccg tttgccgatt acaccgcccg ctacccggac
    13141 gcgccgctgg agcccagcgc cgcccacctg agcccggcca cggtgttcca cgccatgcgc
    13201 gactttgccc tgcgcgtgtc ctggaagcgc gagctgctgg aagacctgga caccctgttc
    13261 tcgggcaaga ccgacgcgcc gattcgccag gcggtggcca atatccacca gcaggtgctg
    13321 cgcggccggg tgtttgtcgc cctgcacatg cacgctggcg acggcaatgt gcacaccaat
    13381 atcccggtga actccgacga ctacgacatg ctgcaaaccg cacacaaggc ggtggcgcgc
    13441 atcatggagc tggcgcgcgg gctgaatggc gtgatctcgg gcgagcacgg cattggcatc
    13501 accaagctgg aatttttgcg cgacgaagaa atcgccccgt ttgtcgccta caagcaacag
    13561 gttgacccga aaggccactt caaccagggc aagctgctgc cgggcgccga cctgcgcaat
    13621 gcctacaccc ccagctttga actgctgggc gcggaaagcc tgattctgga gcaatccgac
    13681 ctgggcgaga tttccgcctc ggtgaaagac tgcctgcgct gcggcaagtg caagccggtg
    13741 tgctccaccc acgtgccgcg cgccaacctg ctgtacagcc cgcgcaacaa gattctgggc
    13801 gtgggcctgt tgaccgaggc cttcctgtac gaagaacaaa cccggcgcgg cgtcagcctc
    13861 aagcactttg acgagctcac cgacgtggcc gaccactgca ccgtctgcca caagtgcgtg
    13921 aacccctgcc cggtgaagat cgactttggc gatgtgtcgg tggccatgcg caacttcctg
    13981 cgcaaggccg gcaagaaaaa attcaacccc ggcaccgccg ccgccatggc cttcctcaac
    14041 gccaaggacc cggccaccat caaggccatg cgtgcgggca tgatggggct gggctacaag
    14101 gcgcagcgcg ccggccacca gctgttcaag cgcctgggcc tgatccagga gcaaaccagc
    14161 cagccgccgt ccacggtggg caagccggcg gtgaagagcc aggtgattca cttcttcaac
    14221 aagccgatgc cgggcaatct gccgaagaaa accagccgcg ccctgctgga catcgaagac
    14281 ccgaacatcg tgccggtgat ccgcgacccg caaaaggcca gcgaaggcgc cgaagcggtg
    14341 ttttacttcc ccggctgcgg ctcggagcgc ctgttcagcc aggtgggcct ggccacccag
    14401 gccatgctct accacgctgg cgtgcaaacc gtgctgccgc ccggctatct gtgctgcggc
    14461 tacccgcaaa acgccaccgg caacgccgac aagggccagg ccatcaccac ggaaaaccgc
    14521 gtgctgttcc accgcgtggc caatacgctg aattatctgg acatcaagac ggtgattgtc
    14581 tcttgcggca cctgcatgga ccagctgcaa aaatacgcct ttggccagat cttccccggc
    14641 tgccggattc tggacatcca cgaatacctg atggaaaagg gcatcaagct cgacggtgtg
    14701 caaggcgtgc gctatatgta ccacgacccc tgccacacgc cgatgaagac ccacgccccg
    14761 ctgaaggtgg tcaacgagct gatgggcagc gaagtgaagc tgaccgaccg ctgctgcggc
    14821 gaatccggca ccctggccgc cacccgcccg gatgtctcca cccaggtgcg cttccgcaag
    14881 gaagaagaca tccgcaaagt ggccgccggc ctgcgcgagc aaagcccgga cagcgaagtg
    14941 aaaatcctca cctcctgccc ggcctgcctg caaggcctgt cgcgctacca cgacgacgcc
    15001 aacgccgacg ccgattacat cgtggtggaa atggccaagg cggtgctggg cgacaactgg
    15061 atggcggatt atgtggccaa ggccagccat ggcggggtgg agcgggtgtt gttgtaagcg
    15121 ccgctcacag ctgcggcgga tgcgtcagtg cgcctccgcc gccctgtcta caccgtattt
    15181 ttttggcccg tcctgcacgg gccatttttc tgaaagccca ccatgacctc agcctgtgaa
    15241 ctctgcgccg ccgacggcgg cgaactgctc taccgcgacg ccaagctgcg catcgtgctg
    15301 gtcaacgacg ccgattaccc cggcttttgc cgggtgatct ggaacgccca tgtgcgcgag
    15361 atgaccgacc tgccagccgt ggaccgctac tacctgctga acaccgtgct gcaagtggaa
    15421 gccctgctgc gcgaggtgtt gcgcccggac aagatcaacc tggccagcct gggcaatgtc
    15481 gtgccgcatc tgcactggca tgtgatcccg cgctttactg acgaccgcca cttcccggcg
    15541 ccggtctggg ccacgccgca gcgcgaagcg gccaccgcgc acaccctgcc ccatctggcc
    15601 gactgcctgc gcgaacggct ggccaccctg catcagcatc cctgagtggt tttttatcat
    15661 tgcatgtcct gaggcctttg ccggtgtctg ccggcaaagg cctcacagtt tctcccttgc
    15721 cgcgtcctct cctgcatgcc tgttcccggt cggtgaatgg cagccggact gcatcgggcg
    15781 cgcggggccc cggcgcagtg gcgttcaccg ccaacttgcc cccaaggagc ccgaaggatg
    15841 gaaatcgaca tgcactacca ggccacctat ctggcggcgc gactggccgg ctttgacaag
    15901 cctcaggcca ccactattgc ccatgccgcc cagtatgtgg acgaatcgga catgagccgg
    15961 ctacaggaca aggacgccgg tttctggatc agggacttca agccgcaccc caccgtgcag
    16021 tccaccaatg agctgatccg cgataccgtc aatctgtgga aatgggacag cagcacccgc
    16081 accggctgga gcgaagccta tctgcgccac ctgcgccggg tatgggcgtg ttttcacttt
    16141 ttgccgggca actatggccc cgacgcaccg tttagctacg aaggcccgac cgaggcgcgt
    16201 ggctggcgtt acgacgacca gtgcgccgaa gaattcagca tgctgtgcct gaccaacagc
    16261 ccgctggtgg ccaatatggt caacgacctg ctcaaccacc aggatcaacc ctacctgccg
    16321 cacctgattg gtgtgcgcat gcatgtgctg gccgatacct gggcacacac ctattttgcc
    16381 ggcacgccgt cgtggtgcgt gaacgaggcg gataatccgg tcacccgggt gtttccggat
    16441 ggcagtaccg cagaaatcaa atgggggccg ggcgggcaag ggcgggaaga attttcgccg
    16501 ggcacctccc tcagctactg gggcatgccc tttctgggcc acggtcgcat ggggcatctg
    16561 ccggattatc cgttcatgcg ctatatgtac ccggccaagt ggtcggggca gccgattttc
    16621 aaaaacaatc cgcgcgacta tctaaacggc atggggcaga tgatccaggc catgcgctgc
    16681 gtgctgaccg gccagccgtt tgtgatcaac caatatgccc cgctgtcgga agacgtgacg
    16741 ttcaagatca atgccctgat tcagatgctg gaaaatacgg atgccagcgt gcgcacacgc
    16801 aagtgggctg aagcactgga cagctggaca ttcgacggcc aatgctttgg cgcgccaccg
    16861 ccattccgcg ccgatgcctg gctggatgaa tacaagcgca cggcactgga aaatcagcct
    16921 ggcaccgatt actaccgctt caaccaggcc gccgtccgcc atgtccagct ggtgggcgat
    16981 gtgctgcgca ccgatgcggc catcaccatt caggaaaacc ccaactgcgc tgtgcagcgg
    17041 gtgcaactgg cctcccgctc gggtcgcccg gtgtatatcg gcccgatgag ccgcagcagc
    17101 acgctgctgg gcggcatcaa gtattgcttc ccacgcgcgg ccacctcgcc gatttccctg
    17161 caactggtga tggtggatgg ccgtcaggca ctggaaaccg gcgggctggt gaaaatcatc
    17221 accgaagaat cggccgtggg cccggaagac tgcctgggcg actggcgcac cagcgattcc
    17281 ctgtattact actatgacgg ctatgccccc acccggcaaa gctggctgct ggaaaaagcc
    17341 gacggcagca agggcccgat ccgctcgggc gacgccatcc gcctgcgcaa tcaggaaacc
    17401 accaaggcca tcagctgcgg acgcgaatgg ctgtccacct cgtccggcac gtccgccgac
    17461 acggagtggg tgattcatta cctgtgagag gggcgtcatg gtgaaaaata ggggtgacgc
    17521 gcaatattgc aaatgaatta aaaagtgcct ttatgtattt tattggcatg atctgagcgc
    17581 tgagcatgaa gcgcctgaag cgcctatccg cgtgttgcga gataggcgct attggcttaa
    17641 gagccgccca taataatcag tgcatgcgga ccgcagacag tacgggcaac gctaggaggt
    17701 aagcggtagg gctcgcatga acgaaatcag cccacatccg caaatgaaca cgctgccagc
    17761 cgctacagac cttctttttt catgattcca tctaaaaaac cacaaatcag agaggatgtg
    17821 gacagtctct gacctgtcga ttgtggataa acatcagtca aatgcacgat aaaagcatct
    17881 gtaccattgc cctgccgtgc tgcgccacag cgctgtctgt gccatgtcgc tagggtcaat
    17941 aggctttttt ttggcatatt gcgtaaaccc acatcacttt gtcatcctcg cccccgcc
//
LOCUS       DAOPYX010000021        17991 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_3276_length_17991_cov_10.377843, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000021 DAOPYX010000000
VERSION     DAOPYX010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 17991)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17991)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17991
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3276_length_17991_cov_10.377843"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(58..318)
                     /locus_tag="PKN55_02130"
     CDS             complement(58..318)
                     /locus_tag="PKN55_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018747794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase assembly factor 2"
                     /protein_id="HNX70074.1"
                     /translation="MSEAEHPFDEIELKRMRWRSRRGLLELDIVIERFLASDFDQLSV
                     ADLHAYRELLTWDDTDLLDLVNCKIEADAPHLQPVIDRLRRA"
     gene            complement(315..1019)
                     /locus_tag="PKN55_02135"
     CDS             complement(315..1019)
                     /locus_tag="PKN55_02135"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009116985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008177 - succinate dehydrogenase
                     (ubiquinone) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HNX70075.1"
                     /translation="MEKMRFSLYRYNPDKDAKPYMQDIEVEVDATDVKLLDALVKLKA
                     VDDTLSFRRSCREGICGSDAMNINGKNGLACVTDLRSLKQPITLRPLPGLPVIRDLIV
                     DMTQFFKQYHSIKPYVVNDTPAPERERLQSPEDRKELDGLYECILCACCSTSCPSFWW
                     NPDKFVGPAGLLAAYRFIADTRDTITSERLDNLEDPYRLFRCHTIMNCVDVCPKGLNP
                     TKAIGKIKDLMVRRAV"
     gene            complement(1035..2801)
                     /gene="sdhA"
                     /locus_tag="PKN55_02140"
     CDS             complement(1035..2801)
                     /gene="sdhA"
                     /locus_tag="PKN55_02140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HNX70076.1"
                     /translation="MSVPVRRFDAVIVGGGGAGLRAALQLSEAGLKTAVLSKVFPTRS
                     HTVAAQGGISASLGNVQEDRWDWHMYDTVKGSDWLGDQDAIEFMCRKAPEAVIELEHF
                     GMPFDRLENGKIYQRPFGGHMANFGKTPVQRACAVADRTGHAMLHTLYQRNVRANTQF
                     FVEWMALDLIRDADGDVVGVTALEMETGEISVLHAKAVLFATGGAGRIFSASTNAFIN
                     TGDGLGMTIRAGIPLEDMEFWQFHPTGVAGAGVLITEGVRGEGGILLNCNGERFMERY
                     APNAKDLASRDVVSRAMAVEIYEGRGCGKNKDHVLLKLDHLGPEIINQRLPGIREIAI
                     KFAGVDPIKEPIPVVPTCHYQMGGIPTNYKGEVVAPKGDNPEDRVNGFYAAGECACAS
                     VHGANRLGTNSLLDLVVFGKSAGESMIEFIRSERPTHKPLPENAADYSLSRIARLENQ
                     TNGEEVADVRTAMQQVVQRHAGVFRTQEVLEEGVKQIKAVAERVKRTQIKDKSQVWNT
                     ARIEALEMDNLIEVALATLISAEARKESRGAHARDDFPDRNDETWMKHTLYSREDFKL
                     SYKPVHNQPLTVDYIAPQKRVY"
     gene            complement(2801..3145)
                     /gene="sdhD"
                     /locus_tag="PKN55_02145"
     CDS             complement(2801..3145)
                     /gene="sdhD"
                     /locus_tag="PKN55_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008953141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, hydrophobic membrane
                     anchor protein"
                     /protein_id="HNX70077.1"
                     /translation="MVNRIVVGAHYGLRDWILQRATAVIMAIYTVGIVLALLAIPGTH
                     EGWKAFFACTAVRVVTQVTLIALFAHVWVGMRDLWMDYIKPVGLRLTMHVFTIVWLVG
                     TLVYSVKVVWGM"
     gene            complement(3139..3513)
                     /gene="sdhC"
                     /locus_tag="PKN55_02150"
     CDS             complement(3139..3513)
                     /gene="sdhC"
                     /locus_tag="PKN55_02150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014086285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b556
                     subunit"
                     /protein_id="HNX70078.1"
                     /translation="MQKKRPKHLDVGQIRLPVPGIVSILHRISGVVLFLFLPVFIYLL
                     SGSLSSAADFETYQAVVGHPLVKLVLIGLLWAFLHHLCAGIRFLFLDIHKGLELETAR
                     ATAKATLVVSIALTLLIGGVLW"
     gene            complement(4101..4826)
                     /locus_tag="PKN55_02155"
     CDS             complement(4101..4826)
                     /locus_tag="PKN55_02155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014086284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HNX70079.1"
                     /translation="MTTDKPSFQPLYQQIRTLLAQRIAQGEWAAHEALPSEWALAESL
                     GVSQGTVRKALTDLVDEGWLYRQQGKGTFVAPGLNEWGDGHLVTPGQFAEPPGSPVPE
                     LLSCTRANASEDIAQALSLRRAAPLFRVRLLWRLAGVAVALDDAYVPVGRFEEIDARR
                     LRQYGNSLYTLLERRDGVRVRLGAIQARATLPDRETMTLLGLTDVEPLLMITRLGQAL
                     TGEAVEWRERLCRSARWAFQREP"
     gene            5073..6053
                     /locus_tag="PKN55_02160"
     CDS             5073..6053
                     /locus_tag="PKN55_02160"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011134617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016615 - malate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HNX70080.1"
                     /translation="MKAPVRVAVTGAAGQIGYALLFRIASGEMLGKDQPVILQLLDLP
                     QAQAAVKGVMMELEDCAFPLLAGMVASDDPNVAFKDADYAILVGARPRSKGMERKDLL
                     EANGAIFTVQGKALNDHASRNVKVLVVGNPANTNAYIAMKSAPDLPAKNFTAMLRLDH
                     NRALSQLAAKTGKAVADIENMAVWGNHSPTMYADYRFATIGGESVKQMINDDVWNSDV
                     FLPTVGKRGAAIIEARGLSSAASAANAAIDHIRDWALGTNGKWVTMGIPSDGSYGIPE
                     GVMFGFPVTCANGEYTIVQGLEIDEFSRGRINVTLNELEEERAGVAHLLG"
     gene            6212..6559
                     /locus_tag="PKN55_02165"
     CDS             6212..6559
                     /locus_tag="PKN55_02165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX70081.1"
                     /translation="MADLSFFTLIAPALFGPSLGGLLDADSSPQQRQAYELVMCADQL
                     GPRYATPYLQAATRLTSAEFVQQALGEARRQGENLRLRAQTQGGGSGPAVADPAQVYQ
                     CQQKLLALPPGQR"
     gene            complement(6560..7651)
                     /gene="rlmM"
                     /locus_tag="PKN55_02170"
     CDS             complement(6560..7651)
                     /gene="rlmM"
                     /locus_tag="PKN55_02170"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012697877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HNX70082.1"
                     /translation="MTVSVSPAQGWVLYCRPGFERDCAQEAYLHALRQGAELRIAEAV
                     ENSGYVRLEGRARAPDWSALVFARQALSLLAMVELPERDRLTPLLDALPAQPAVFADV
                     WLEMPDTNDGKALSAFTRRFAPLLQDALIDQRRLGGRPDGPRLHVFFPDKQRAWLALG
                     DPRLSAPWPMGILRLRMPPDAPSRSALKLAEAFDMFLSAEDQQRYLHDGLRAVDLGAA
                     PGGWTWQLLRRGLRVIAVDNGPLKGMVAEHPWVTHLRTDGFRFRPDRPVDWVVCDMVE
                     KPSRVATLMANWLIGDHARHAMFNLKLPMKKRQEALESALNDIETLMQAHDIRYRLSV
                     KQLYHDREEVTVYLGREARPRRNGNSKRR"
     gene            complement(7687..8598)
                     /locus_tag="PKN55_02175"
     CDS             complement(7687..8598)
                     /locus_tag="PKN55_02175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012696205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HNX70083.1"
                     /translation="MWFKQLTLYRLDPQALPALDALEAALQQRPFAPCAGLDWFTQGF
                     VPAARHQPDLMLFRQGEMALVALRRQEKVLPASVIRDLTDERVQEIEARDVRRVGKKE
                     KQQLREQVADELLPRAFSRNLHVRAWLDLAHGWLAVDAGSAARAETLLGALRDALPPF
                     PARLPRTRLSPATQMTAWLQGEAPPGFALDADCELKAPGEGGAVVRCSRQDLTASEVR
                     AHLDSGKQVSKLGLIWQERIRFVLTEDLGVRRVQFLDLLQEEAEQAGDDAESLFEATF
                     ALMTGELAVLIADLIDALGGEPQDQDA"
     gene            8830..8906
                     /locus_tag="PKN55_02180"
     tRNA            8830..8906
                     /locus_tag="PKN55_02180"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8864..8866,aa:Pro,seq:cgg)
     gene            complement(9129..10082)
                     /locus_tag="PKN55_02185"
     CDS             complement(9129..10082)
                     /locus_tag="PKN55_02185"
                     /inference="COORDINATES: protein motif:HMM:NF013720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate-binding protein"
                     /protein_id="HNX70084.1"
                     /translation="MSATAPLPDYPAAGAASWCELNEFAVLDFYGEDAPSFLQGQLSS
                     DVREVSEQHAQYSSYSTAKGRMLASFLIWRRDEHYRLLVSADLAEAMKKRLGMFILRA
                     KVKHAIDADTVLLGVQGEAGALEAALGVALPQEVGAVAQAAGGSVLALAGGRCLLALS
                     ADAAPAVRAALAEAGLTQTTSAAWRAADIAHGLPWISVATQEMFVPQMANMELIGAVN
                     FKKGCYPGQEIVARSQYLGKVKRRMFRVRIADPAAAPGAELFSPASGEQVIGNLVNVA
                     PVDGGVEALAVFQLPALEAGVHLGSLSGPRLEVLTLPYSVE"
     gene            complement(10226..10492)
                     /gene="rpsT"
                     /locus_tag="PKN55_02190"
     CDS             complement(10226..10492)
                     /gene="rpsT"
                     /locus_tag="PKN55_02190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017508131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HNX70085.1"
                     /translation="MANSAQARKRARQAEVAREHNASLRTQYRTAVKKVLKAVLAGDK
                     AAAQEVFKSSVAVIDRIADKKVVHKNKASRHKSRLSAKIKALAA"
     gene            10653..12191
                     /gene="murJ"
                     /locus_tag="PKN55_02195"
     CDS             10653..12191
                     /gene="murJ"
                     /locus_tag="PKN55_02195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HNX70086.1"
                     /translation="MNLLQTLARVSSMTLVSRILGFARDAIIARAFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPVLAEYKEKQGEDAARALLANVAGVLTLALAAVTVLG
                     MLAAPWVIYLSAPGFGGDGDKFALTVTLLRITFPYILLISLASLASSVLNTWRQFSIP
                     AFTPTLLNISFIVFSTLLAPYFDPPVLALGWAVFAGGVAQLAFQLPYLRRIRMLPWPK
                     VNFSDAGVNRILKQMGPAIFGVSIGQVSLVINTIFASLLVSGSVSWMYYADRLMEFPT
                     GVLGVALGTILLPSLSRHAAQADDGEFSRLLDWGIRLSLLLALPASVGLAVLSEPLIA
                     TLFMYGKFNAHDVAMTQQALIAYAIGLVGLILVKVLAPGFYARQNVKTPVRIAMITLG
                     CTQVMNLVFIIPLKHAGLSLAIGLGACLNAGLLLYQLRKQGIYQPQDDWGRFARRLLL
                     AVLVMAEVLLALNWGSPFDWAHQPALTRTLWLTAMVVMGVIVYFGMLYAQGFRLADFR
                     RKEH"
     gene            complement(12287..14362)
                     /locus_tag="PKN55_02200"
     CDS             complement(12287..14362)
                     /locus_tag="PKN55_02200"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNX70087.1"
                     /translation="MMTPADTPLHEAISLMREQHVEALLVCYPDSDYGILTQRDVVRL
                     LAEKTSHLPVGAVASHPIISVPKQTSLYYARKLLAEKHIRHLGVLDRGELVGVISFSD
                     ILTSIEHEYVNELQYALKERDEALQISRQNLRMADKVFESTLEGIMITDANGVIETVN
                     PAFSRITGYRKSEVVGRNARILSSGKQPPEFYRHLWISLRENGFWQGELWNRCKNGTL
                     FHEYLTITGIKDETGQYSHFAGIFTDITQRKIAEERLHFLANHDPLTGLPNRTLFMER
                     LLLATQRAQREGSKIGLMFIDLDRFKYINDTMGHAAGDKLLTEIAERLKSTLRECDTV
                     ARLGGDEFTVILENISDVQHVAKIAKKLVSKLNGIVKIEQQEVFVTASIGIAIFPEDG
                     EAPETLLMNADTAMYRAKERGKNNFQFFTSDMNTSTMERMRLENSLHAALSRSELLLH
                     YQPKLKLDTGELFGYEALIRWNHPQMGMVSPAQFIPIAEDSSLIVPIGDWVLHTACAQ
                     ARRWLDRGDLLGRMAVNISHRQLKLANLVDSVHSALQHSGLPAECLELEITESMAMDT
                     TQETLDTLVRLREMGVHLTIDDFGTGYSSLSYLRKLPIDGLKIDRSFVAGVVQEPGDA
                     AITRAIISLAASLGLAVTAEGIEEASQLAFLREQGCPFGQGYFYCRPMDVARLDDWLA
                     ARRRELAAH"
     gene            complement(14426..14869)
                     /locus_tag="PKN55_02205"
     CDS             complement(14426..14869)
                     /locus_tag="PKN55_02205"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNX70088.1"
                     /translation="MSLDLTIPILEPFTDASLGELPAVPVRRTIEQIISPRIFSCQPE
                     TSVVEAARLMREAACGSLLVIEDTRAVGIWTETDALRLNFADPATFELSVRDVMSWPV
                     KTISVDTTFNDAAVLFKRHGVRHYLVVDDQGGYRGILSQSDIVLN"
     gene            complement(15138..16319)
                     /locus_tag="PKN55_02210"
     CDS             complement(15138..16319)
                     /locus_tag="PKN55_02210"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020704072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HNX70089.1"
                     /translation="MQEVVIVAATRTAIGSFGGSLAKISAPDLGAIVIKSLLEKTGVA
                     PEAVSEVILGQVLTAGVGQNPARQALIKAGLPVTTPASTLNVVCGSGLRATHLAAQAI
                     ANGDAEIVIAGGQENMSMSPHILPGSRDGFRMGNAQLVDTMVYDGLTDAYNQYHMGIT
                     AENIAAKYEISRDEQDQLAVTSQNRAEAAQKAGKFADEITPVLIPQRKGDPVAFVNDE
                     FIKHGATLEGLGKLRAAFKKDGTVTAGNASGINDGAAAVLMMSAAKAAELGLTPMARV
                     AGYALSGCAPEIMGMGPVSASQKALAKAGWAVEDLDLVEANEAFAAQALGVTRELKWD
                     LNKVNVNGGAIALGHPIGASGCRVLVTLLHEMQRRDAKKGLATLCIGGGMGVALAVER
                     A"
     gene            complement(16396..>17991)
                     /gene="phaC"
                     /locus_tag="PKN55_02215"
     CDS             complement(16396..>17991)
                     /gene="phaC"
                     /locus_tag="PKN55_02215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014086688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042619 - poly-hydroxybutyrate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I poly(R)-hydroxyalkanoic acid synthase"
                     /protein_id="HNX70090.1"
                     /translation="QLVEMQTNLYQQQMNLWMNFLGQKVPEVASPDPSDKRFAAAEWN
                     DHPFFNLLKQNYLFASKWLTEMVDGVPIDAHQKERMSFFTKQYLDALSPTNFAMTNPE
                     VLKRAIETKGESLVEGMKHMMADMEKGYISMSDESKFEIGKNIAATPGSVIFRNELIE
                     LIQYTPTTEKVYERPLLIIPPCVNKYYLMDLGPDNSMVRHFVSQGYTVYLVSWKSADR
                     EMKHFTWDTYIDKGAIAAIDTVRKISKQDKINVLGFCIGGVILNTTLCVLKDRGLDWV
                     ESATFMTSLVDHTDPGEIRMYIDENLIRSRETKVAAGGIVSGKEIGQTFASLRANDLV
                     WNYVVNNYLLGKTPPPFDLLYWNNDSVDLPLPMHTFFLKEFYLNNALTKKGAITLCGV
                     PIDVSTIELPLYIFAAREDHIVPWTSAYDGLKYLTGAKDKRYVLGASGHIAGAINPVT
                     KNKRNYWVNDNRPATAQEWLAGAESRPGSWWQDWDQWLAPRSGAQVAAPKTPGNKDFK
                     PLCAAPGTFVLARSMPQTAAMMA"
BASE COUNT         3211 a         5601 c         5710 g         3469 t
ORIGIN      
        1 tcccaggtag ggttttaagg gcgcccctgt ccgcaccaca cggacggggc gtcttgttta
       61 agcgcgacgc agccggtcga tgaccggttg caggtgcggg gcgtcggctt cgatcttgca
      121 gttgaccagg tccagcaggt cggtgtcgtc ccaggtcaac agctcgcggt aggcgtgcag
      181 atccgccacg ctgagctgat cgaagtcgct ggccagaaag cgctcgatca cgatgtcgag
      241 ttcaagcagc ccacgccggg aacgccagcg catgcgcttg agctcgatct cgtcgaacgg
      301 gtgttcggcc tcgctcatac ggcgcgacgc accatgaggt ctttgatctt gccgatggcc
      361 ttggtcgggt tcaggccctt cggacacacg tccacgcagt tcatgatggt gtggcaacgg
      421 aacaggcggt acgggtcttc caggttgtcc agacgctcgc tggtgatggt gtcgcgggta
      481 tcggcaataa agcgatacgc ggccaacagg ccagccgggc cgacgaactt gtccgggttc
      541 caccagaacg acgggcagga ggtcgagcag caggcgcaca gaatgcactc gtacaggcca
      601 tccagctctt tgcggtcttc cggcgattgc agacgctcac gctccggcgc cggggtgtcg
      661 ttgaccacat agggcttgat cgagtggtac tgcttgaaga actgggtcat gtccacgatc
      721 aggtcgcgga tgaccggcag gccaggcagc gggcgcaggg tgatcggctg cttgaggctg
      781 cgcaggtcgg tcacgcaggc caggccgttc ttgccgttga tgttcatcgc gtccgagccg
      841 cagatgcctt cgcggcagga gcgacggaag gacagggtgt catccaccgc cttcaacttg
      901 accagggcgt ccaggagctt gacatcggtg gcgtcaactt cgacttcgat gtcctgcatg
      961 tagggcttgg cgtccttgtc cgggttgtag cggtaaaggg agaaacgcat tttttccatg
     1021 atgtcggcgg ctccttagta cacgcgcttt tgcggggcga tgtaatccac cgtcagcggc
     1081 tgattgtgca ccggcttgta gctgagcttg aagtcttcgc gcgagtacag cgtgtgcttc
     1141 atccaggtct cgtcgttgcg atccgggaaa tcatcgcggg cgtgggcgcc acgggattcc
     1201 ttgcgcgctt cggcggagat cagggtggcc agggccactt cgatcaggtt gtccatttcc
     1261 agcgcttcga tgcgggcggt gttccagacc tgggacttgt ccttgatctg ggtgcgcttg
     1321 acgcgctcgg ccacggcctt gatctgcttg acgccttctt ccagcacttc ctgggtgcgg
     1381 aacacgccag cgtgacgctg caccacttgc tgcatggcgg tgcgcacatc ggccacttct
     1441 tcgccattgg tctggttttc cagacgggca atgcgcgaca gcgagtagtc agcggcgttt
     1501 tccggcagcg gcttgtgggt cgggcgctcg ctgcggatga attcgatcat gctttcgccg
     1561 gcagacttgc cgaacacgac cagatccagc agcgagttgg tgcccaggcg gttggcgccg
     1621 tgcaccgagg cgcaggcgca ttcgccagcg gcgtagaagc cgttgacgcg gtcttccggg
     1681 ttgtcgccct tcggtgcgac cacttcgccc ttgtagttgg tcggaatgcc gcccatctgg
     1741 taatggcagg tcggcaccac cgggatcggc tctttgatcg gatccacgcc agcgaatttg
     1801 atggcgatct cgcggatgcc aggcagacgc tggttgatga tttccgggcc gaggtgatcc
     1861 agcttgagca gcacgtggtc tttgttcttg ccacagccac ggccttcgta gatttccacg
     1921 gccatggcgc gggacaccac gtcacgcgaa gccagatcct tggcgttcgg cgcgtagcgc
     1981 tccatgaagc gctcgccatt gcagttgagc agaatgccgc cttcgccgcg cacgccttcg
     2041 gtgatcagca cgcccgcgcc ggccacgccg gtcgggtgga attgccagaa ttccatgtct
     2101 tccagcggaa tgccggcgcg aatggtcatg cccaggccgt caccggtgtt gatgaaggcg
     2161 ttggtcgatg cggagaaaat acggccagcg ccgccagtgg cgaacagcac cgccttggcg
     2221 tgcagcaccg agatttcgcc ggtttccatt tccagcgcgg tcacgcccac cacgtcgccg
     2281 tcggcgtcgc ggatcaggtc cagcgccatc cattcgacaa agaactgggt gttggcgcgc
     2341 acattgcgct ggtacagcgt gtgcagcatg gcgtggccag tgcggtcagc cacggcgcag
     2401 gcgcgctgca ccggggtctt gccgaagttg gccatgtggc cgccgaacgg acgctggtaa
     2461 atcttgccgt tttccagacg gtcaaacggc atgccgaagt gctcgagctc gatcacggct
     2521 tcgggcgcct tgcggcacat gaattcgatg gcgtcttggt cgccgagcca gtcggaaccc
     2581 ttgacggtgt cgtacatgtg ccagtcccaa cggtcttcct gcacattgcc caacgatgca
     2641 gaaatgccgc cctgggccgc cacggtgtgg gagcgggtcg ggaacacttt ggagagaaca
     2701 gcggtcttca gacccgcttc agacagctgc agcgccgcgc gcaggccggc gccgccgcca
     2761 cccacgatca ctgcgtcaaa acgacgaact gggacgctca ttacataccc cacacgactt
     2821 taaccgaata aacaagagtg cccaccagcc agacaatggt gaacacatgc atggtcaggc
     2881 gcaggccgac cggcttgatg tagtccatcc acaggtcgcg catgccaacc cagacgtggg
     2941 cgaacagggc gatcagggtc acttgggtga ccacacgcac ggcggtacag gcaaagaacg
     3001 ccttccagcc ttcatgggtg ccggggatgg ccagcaacgc cagcacgatg ccgacggtgt
     3061 aaatcgccat gatcacggcg gtggcgcgct ggagaatcca gtcacgcagg ccgtaatggg
     3121 cgccaacgac gatgcggttt accacagcac acctccgatc agcagggtca atgcgatgct
     3181 caccaccagg gtggccttgg cggtggcgcg ggcggtttcc agctcaagac ccttgtgaat
     3241 gtcgaggaac aggaagcgga tgccggcgca cagatggtgc aggaaggccc acaacaggcc
     3301 gatcagcacc agtttgacca gcggatgacc gaccacggcc tggtaggttt cgaagtccgc
     3361 cgcagaactg agcgagccgg acaacagata gatgaagacg gggaggaaca ggaacaggac
     3421 aacacccgag atccgatgca ggatcgagac aatgccggga accggcaatc tgatctgacc
     3481 gacgtcgagg tgttttggtc gtttcttctg catagcgctt ccttgatgaa acaagacacc
     3541 tgaggtttga catttccggg gcgtcgcccc gggccttgaa gccgacgtcg aacccgacgc
     3601 gaaggctccg tgtgctgact gcttgccgct tttcgccgct catggcggcg ggcgctgacg
     3661 ccggcaatcc agaagaccgc cgccgccaaa gctgtcggac gcttcatgcc gtccgagctc
     3721 gccgcagcaa aatccctgca aaaacagggg caacaccgtg tttcatgacc cgcccccgca
     3781 caggcgacag ccggccaaac ctgcaaattc tatcaaaaaa ccaagcgccc ccgcatcctg
     3841 ttacagaacg acgacatcga ttttcatctt cgcgccatgc agtgtgttcc cgcagcgctg
     3901 ctggcctggc ttttgaacag ccgaccagtc ttgtgtcatg acggcgtccg gacgggtgcg
     3961 gacggccggt caagaccgat ccgcccacac ctgccgggcc gactgcgacc gggtgaaccc
     4021 ggttctgtga ttggcggtct tgcgacctgt ctcgcggggc cgccctggcg ggcggcgact
     4081 ccatcccatc gaatgctgta ttacggttcg cgctgaaaag cccagcgcgc gctgcggcat
     4141 aaacgctcgc gccattccac cgcctcgccg gtcagcgcct gccccagccg ggtgatcatc
     4201 agcagcggct cgacgtcggt cagccccagc aaggtcatgg tttcccggtc tggcagcgtg
     4261 gcgcgcgcct gaatcgcccc caggcgcacc cgcacgccgt cgcggcgctc cagcagggtg
     4321 tacaggctgt tgccgtactg acgcaggcgg cgggcgtcga tttcttcaaa gcgccccacc
     4381 ggcacataag catcatccag cgccaccgcc accccggcca gacgccacag caggcgcacc
     4441 cgaaacagcg gcgcggcgcg gcgcaggctc agcgcctggg cgatgtcctc gctggcgttg
     4501 gcgcgggtgc acgacagcaa ctcgggcact ggcgagccag gcggctcggc aaactggccg
     4561 ggggtgacca gatggccatc gccccattca ttcaagcccg gcgccacaaa ggtgcccttg
     4621 ccttgctggc gatacaacca gccttcgtcc accaaatccg tcagcgcctt gcgcaccgtg
     4681 ccctggctga cgcccagcga ttcggccagc gcccactcgc tgggcaaggc ctcgtgcgcc
     4741 gcccattcgc cttgcgcaat gcgctgcgcg agcagcgtgc gtatttgctg gtacagcggc
     4801 tggaaactgg gcttatccgt tgtcatcgac gtgtttttac acctttctgt cagggttgtc
     4861 catgcatgtc ttatataaga cataacacta gcacgatcct cctgtttgcg catggcaagt
     4921 caggggggtt tagtgttaca ctcggacaag catgtgatgg gcacagaagg agagcggcgc
     4981 acctgacgcc gacacgccgg cccgcgccgg ccgcatcgtg tctcgcgcag catgcttaaa
     5041 tcccgttccg tatacaaatg gagagtcaat cgatgaaagc ccccgttcgt gttgccgtca
     5101 ccggcgctgc tggtcaaatt ggttacgccc tgctgttccg catcgcctct ggcgaaatgc
     5161 tgggcaaaga ccagccggtg atcctgcaac tgctggacct gccgcaagct caggccgctg
     5221 ttaaaggcgt gatgatggaa ctggaagact gcgccttccc gctgctggcc ggcatggtgg
     5281 ccagcgacga tccgaacgtg gctttcaagg atgccgacta cgccatcctg gtgggcgccc
     5341 gtccgcgcag caagggcatg gagcgcaagg atctgctgga agccaacggc gccatcttca
     5401 ccgtgcaggg caaggcactg aacgaccacg ccagccgcaa cgtcaaggtg ctggtggtgg
     5461 gcaacccggc caacaccaac gcctacatcg ccatgaagtc cgccccggat ctgccggcca
     5521 agaacttcac cgccatgttg cgtctggacc acaaccgcgc cctgtcgcaa ctggccgcca
     5581 agactggcaa ggccgtggcc gacatcgaaa acatggccgt gtggggcaac cacagcccga
     5641 ccatgtacgc tgactaccgc tttgccacca tcggcggcga gagcgtcaag caaatgatca
     5701 acgacgacgt gtggaacagc gacgtgttcc tgccgaccgt cggcaagcgc ggcgccgcca
     5761 tcatcgaagc ccgtggcctg tcctccgccg cctccgccgc caacgccgcc atcgaccaca
     5821 tccgtgactg ggcgctgggc accaacggca agtgggtgac catgggcatc ccgtccgacg
     5881 gttcctacgg cattccggaa ggcgtgatgt tcggcttccc ggtgacctgc gccaatggcg
     5941 aatacaccat tgtgcaaggc ctggaaatcg acgaattcag ccgtggccgc atcaacgtca
     6001 ccctgaacga gctggaagaa gagcgcgctg gcgtggctca cctgctgggt tgattcccac
     6061 ctgtcgctca cccggcgtca gccgggcggg cgcagaaaaa aacgcaccgt caacccatga
     6121 cggtgcgttt tttgtttttg ccgcggcgct tgccgctgcg catggcctga tcgcatgcgc
     6181 tacactgcgc catcttcacc cgagcatccc catggccgac ctcagctttt tcaccctcat
     6241 cgcccccgcc ctgtttggcc cgtcgctggg cggtttgctg gatgccgaca gtagcccgca
     6301 gcagcgccag gcttacgagc tggtgatgtg cgccgaccag ttggggccgc gctatgccac
     6361 gccgtatttg caggcggcca cgcggctgac atcggcggag tttgttcagc aggcgctggg
     6421 ggaagcgcgg cggcagggtg agaacctgcg cttgcgcgcg cagacgcaag gtggcggcag
     6481 cgggccggcg gtggccgacc cggcgcaggt gtatcagtgc cagcaaaagc tgctggcact
     6541 gccgccgggt cagcgctgat cagcgccgct tgctattgcc attgcggcgc gggcgcgctt
     6601 cgcggcccag gtagacggtg acttcttcgc ggtcgtgata cagctgcttg accgacaggc
     6661 gatagcggat atcgtgcgcc tgcatcaggg tttcgatgtc gttcagcgcg ctttccagcg
     6721 cttcctgacg ttttttcatc ggcagtttca ggttgaacat ggcgtggcgg gcgtggtcgc
     6781 caatcagcca gttggccatc agcgtggcca cccgcgaggg tttttccacc atatcgcaca
     6841 ccacccagtc caccgggcga tccgggcgga agcgaaaacc gtcggtgcgc agatgggtca
     6901 cccaggggtg ttcggccacc atgcccttga gcgggccgtt gtccacggca atcacccgca
     6961 ggccacggcg cagcagctgc caggtccagc cgccgggcgc ggcgcccagg tccaccgcgc
     7021 gcaggccgtc gtgcagatag cgctgctggt cttcggcgga caaaaacata tcaaacgctt
     7081 cggccagttt cagcgccgag cggctgggcg cgtcgggcgg catgcgcagg cgcaggatgc
     7141 ccatcggcca cggcgcgctc agccgcgggt cgcccagcgc cagccaggcg cgctgtttat
     7201 ccggaaaaaa cacatgcagg cgcgggccat ccgggcggcc gcccagacgg cgctggtcaa
     7261 tcagcgcgtc ctgcaataag ggcgcaaagc ggcgggtaaa cgccgacagc gccttgccgt
     7321 cgttggtgtc cggcatttcc agccagacat cggcaaacac tgccggttgc gccggcagcg
     7381 cgtccagcag cggggtcagc cggtcgcgct cgggcagctc caccatggcc agcaaggaca
     7441 gcgcctggcg ggcaaacacc agcgcgctcc agtctggcgc tcgggcgcgg ccttccaggc
     7501 ggacatagcc gctgttttcc acggcttcgg caatgcgcag ctcggcgccc tggcgcaggg
     7561 cgtgcaaata ggcttcttgt gcgcaatcgc gctcaaagcc ggggcggcaa tacaacaccc
     7621 agccctgggc tggggaaaca gaaacagtca tcgggtcgtc aattcagagt ggaaaggcgc
     7681 gggcgttcag gcgtcctggt cttgcggttc gccgcccagc gcgtcgatca aatcggcaat
     7741 cagcaccgcc agctcgccgg tcatcagcgc aaaggtggct tcaaacagcg attcggcgtc
     7801 gtcgccggcc tgctcggctt cttcttgcag caggtcaaga aactgcacgc ggcgcacgcc
     7861 caggtcttcg gtgagcacaa agcggatgcg ctcctgccag atcaggccca gcttgctcac
     7921 ctgcttgccg ctgtccagat gggcgcgcac ttcgctggcg gtgaggtcct gacggctaca
     7981 gcgcaccacg gcgccgcctt cgcccggcgc cttgagctcg caatcggcgt ccagggcaaa
     8041 accgggcggg gcttcgcctt gcagccaggc ggtcatttgc gtggcgggcg acagccgggt
     8101 gcgcggcaaa cgcgccggaa acggcggcaa ggcgtcgcgc aaggcgccca gcagggtttc
     8161 ggcgcgggcg gcgctgccgg cgtccaccgc cagccagccg tgcgccaagt ccagccaggc
     8221 gcgcacatgc agattgcggg aaaacgcgcg cggcagcagt tcgtcagcca cctgctcgcg
     8281 cagttgctgt ttttcttttt tgcccacccg ccgcacatcg cgggcttcga tttcttgcac
     8341 gcgctcgtcg gtcaggtcgc ggatcacact ggcgggcaag accttctcct ggcggcgcaa
     8401 ggccaccagc gccatttcgc cctggcggaa cagcatcagg tcaggctgat ggcgcgcagc
     8461 cggcacaaaa ccctgggtga accagtccag cccggcgcag ggcgcaaacg ggcgctgttg
     8521 cagcgcggcc tccagcgcgt ccagcgccgg cagggcctgg ggatcaaggc gatacagggt
     8581 gagttgtttg aaccacatca gaggcgtcca cgcaaaaaga cttgggcagc ggattgtagc
     8641 cgagctgcgc catccgcagg aaataaaccg gcaaagccgc tgtttggccg gcggataagt
     8701 gactgatttt tcatcttctg caaaatttct ctcatttttt tgcaaaaatc ccttgccaag
     8761 ccataagtca tcccctaata tgcgcatctc tcagcgacgc aatgcaacaa agcagtcaac
     8821 gagaaaattc ggggtgtagc ttagcctggt agagcgctac gttcgggacg tagaggccgg
     8881 aggttcgaat cctctcaccc cgaccagatt cgagaaaaac ggaaacatct tcacggtgtt
     8941 tccgtttttt gttttgtccg ttcgccagcg ccgcacgccg cccaggcggc gccggcaccc
     9001 ggcgccgccc aagccagagc ggctaacaaa acccagcgaa gcgatcctgg ctaggcgcgc
     9061 gaccgcagac agtacgggta gtacggcaag gtcgggcaac aacgccagga gggttttgtt
     9121 agcggctctt attccaccga atacggcagg gtcagcacct ccagccgcgg gccgctcaaa
     9181 ctgcccagat gcacgcccgc ttccagcgcc ggcagctgaa acaccgccag cgcctccacg
     9241 ccgccatcca ctggcgcgac attcaccaga ttgccaatca cctgctcgcc gctggccggg
     9301 ctgaacagct cggcgcccgg cgcggcggcc gggtcggcga tgcgcacgcg aaacatccgg
     9361 cgcttgacct tgcccaggta ctgcgagcgg gcaacgattt cctgaccggg atagcagcct
     9421 tttttgaagt tcaccgcgcc aatcagctcc atattggcca tctgcggcac aaacatttct
     9481 tgcgtggcga cgctgatcca cggcaggcca tgggcaatgt cggcggcgcg ccaggcggcc
     9541 gacgtggttt gcgtcagccc ggcctcggcc agcgcggcgc gcaccgccgg ggcggcgtcg
     9601 gcgctcagcg ccagcaggca gcgcccaccg gccagggcca gcacgctgcc gcccgccgcc
     9661 tgcgccaccg cgcccacttc ttgcggcaag gccacgccca aagccgcctc cagcgcgccc
     9721 gcctcgcctt gcacgcccag cagcacggtg tcggcgtcga tggcgtgctt gaccttggcg
     9781 cgcaggatga acatgcccag gcgctttttc atcgcctcgg ccaggtccgc cgacaccagc
     9841 aagcggtaat gctcatcgcg ccgccagatc agaaaactgg ccagcatgcg gcccttggcg
     9901 gtggaatagc tggaatactg ggcgtgttgt tcactgactt cgcgcacatc gctggacagc
     9961 tggccttgca aaaagcttgg cgcatcttct ccgtaaaaat ccagcacggc aaattcgttc
    10021 agctcacacc agctggcggc gccagcggca gggtaatcgg gcaggggggc agtggcgctc
    10081 atgacgggct cctcattcag gatggcaaaa cgggggaatc agatagacat cgcggcaaat
    10141 ggcgcggatt caagaggacg caaaccgcca ataaaaaacc ccgctcgtgg gcggggtttt
    10201 ttattgcagc aagaacgcgc aacaatcagg cagccagggc cttgatcttg gcggacaggc
    10261 ggctcttgtg gcgagaagcc ttgttcttgt gcacaacttt cttgtcggcg atgcggtcga
    10321 tcacggcgac ggaagacttg aacacttctt gagcggcggc cttgtcgccg gccagcacag
    10381 ccttcagcac tttcttgacg gcggtacggt attgggtacg caggctagcg ttatgctcgc
    10441 gggccacttc ggcctggcga gcgcgcttgc gagcttgtgc gctatttgcc atggatgtct
    10501 cctgggacgg attctgtaaa actcgcgatt gtacttacca cggataggtt tggcaagctc
    10561 aagcccttgt tgccccatgt gattctgccg gcgtcagagc ggaaatcgta taaaatgccg
    10621 gctttgccgc tggtcggcgt gttgattgtg ttatgaacct actgcaaacg ctggccagag
    10681 tcagcagcat gaccctggtt tcgcgcattc tcggctttgc ccgcgacgcc atcattgccc
    10741 gcgcctttgg cgccggcatg gccactgacg cgttttttgt ggcgttcaag ctgcccaatc
    10801 tgctgcggcg catttttgcc gaaggcgcgt tctcgcaggc ttttgtgccg gtgctggccg
    10861 aatacaaaga aaaacaaggc gaagacgccg cgcgcgcgct gctggccaat gtggccggcg
    10921 tgctgacgct ggcgctggcg gcggtcacgg tgctgggcat gctggcggcg ccctgggtga
    10981 tttacctgtc ggcgccaggc tttggcggcg atggcgacaa gtttgccctc accgtcaccc
    11041 tgctgcgcat cacttttccg tatatcttgc tgatttcgct ggcctcgctg gccagcagcg
    11101 tgctgaacac ctggcggcag ttttccattc cggccttcac ccccaccttg ctgaacatca
    11161 gctttattgt gttttccacc ctgctggcgc cgtatttcga cccgccagtg ctggcgctgg
    11221 gctgggcggt gtttgccggc ggcgtggcgc agctggcgtt tcagctgccg tatctgcgcc
    11281 gcatccgcat gctgccgtgg cccaaggtga atttctctga cgccggggta aaccgcattc
    11341 tcaagcagat gggcccggcg atttttggcg tgtcgattgg ccaggtgtcg ctggtgatca
    11401 acaccatttt tgcctcgctg ctggtgtccg gcagcgtgtc gtggatgtat tacgccgacc
    11461 ggctgatgga attccccact ggcgtgctgg gcgtggcgct gggcaccatc ttgctgccat
    11521 cgctgtcgcg ccacgccgcc caggccgatg atggcgagtt ttcccgcttg ctggactggg
    11581 gtatccgcct gtcgctgctg ctggcactgc cggcgtcggt gggcctggca gtgctgtccg
    11641 agcccttgat cgccacctta ttcatgtatg gcaagttcaa cgcccacgat gtggcaatga
    11701 cccagcaggc gctgattgcc tacgcgattg gcctggttgg gctgattctg gtcaaggtgc
    11761 tggcgccggg gttttacgcc cggcagaacg tgaaaacccc ggtgcgcatt gccatgatca
    11821 ccctgggctg cactcaagtg atgaatctgg tgtttattat tccgctcaag cacgccgggc
    11881 tgtcgctggc cattggcctg ggcgcctgcc tgaacgccgg gctattgctg taccaactgc
    11941 gcaaacaggg catttatcag ccgcaggacg actgggggcg ctttgcccgc cgcttgctgc
    12001 tggcggtgct ggtgatggcc gaggtgctgc tggcgctgaa ctgggggtcg ccgtttgact
    12061 gggcgcatca gccggcgctg acgcgcacgc tgtggctgac tgccatggtg gtgatgggag
    12121 tgattgtgta ctttggcatg ctgtacgccc agggtttcag gctggcggat ttccggcgca
    12181 aggaacactg agcgctgctg aaaaaacgcg cctggccgtg ccgtgttgca ctgtctgcgc
    12241 gcgccctcag tccctgccgc gccgcgcttg gcgggcgtcg ccccgctcag tgcgccgcca
    12301 actcgcgccg gcgcgccgcc agccagtcgt ccagccgggc gacgtccatc ggccggcaat
    12361 aaaaatagcc ctggccaaac gggcagccct gctcgcgcaa aaaggccagc tgactggctt
    12421 cttcgatgcc ctcggcggtc accgccagcc ccagactggc ggccagcgag atgatggcgc
    12481 gggtgatggc ggcatcaccg ggctcttgca ccacgccagc cacaaaagag cggtcgatct
    12541 tcaggccgtc aatcggcagc ttgcgcagat aagacagcga agaatagcca gtgccaaagt
    12601 cgtcgatggt caggtgcacg cccatttcac gcaggcgcac cagggtgtcc agcgtttcct
    12661 gcgtggtgtc catcgccatg ctttcggtga tttccagctc caggcattcc gctggcaggc
    12721 cgctgtgttg cagcgcgctg tgcacgctgt ccaccagatt ggccagcttg agctggcgat
    12781 gcgagatatt caccgccatg cgcccaagca agtccccacg atccagccaa cgccgcgcct
    12841 gcgcgcaggc ggtgtgcaat acccagtcgc caatcggcac gatcagcgaa gaatcttccg
    12901 caatcgggat aaactgcgcc ggcgacacca tgcccatttg cgggtgattc cagcggatca
    12961 gcgcctcgta gccaaacagc tcgcccgtgt ccagcttgag cttgggctgg tagtgcagca
    13021 agagctcgct gcgcgacagc gcggcgtgca ggctgttttc caggcgcatg cgctccatgg
    13081 tcgaggtgtt catgtccgag gtgaagaact gaaagttgtt cttgccgcgc tccttggccc
    13141 gatacatcgc cgtgtcggcg ttcatcagca gggtttcggg cgcttcgcca tcctcgggga
    13201 agatggcaat gccaatcgag gcggtgacaa acacctcctg ctgttcgatc ttgacgatgc
    13261 cattgagctt ggacaccagt ttcttggcga tcttggccac atgctggacg tcggagatat
    13321 tctccagaat cacggtaaac tcatcgccgc ccagccgcgc cacggtgtcg cattcacgca
    13381 gcgtgctttt cagccgctcg gcgatttcgg tcagcagctt gtcgcccgcg gcatggccca
    13441 tggtgtcgtt gatgtacttg aagcggtcca gatcaatgaa catcaggccg atcttgctgc
    13501 cttcgcgctg cgcacgctgg gtggccagca acaggcgctc catgaatagc gtgcggttgg
    13561 gcaggccggt gagcgggtcg tggttggcca gaaaatgcag gcgttcttcg gcgattttgc
    13621 gctgggtgat gtcggtgaag atgccggcaa agtgcgagta ttggccggtt tcgtctttga
    13681 tgccggtgat ggtcaggtat tcgtgaaaca gcgtgccgtt tttgcagcgg ttccacagct
    13741 cgccctgcca gaagccgttt tcgcgcaagc tgatccacag gtggcgataa aactcgggcg
    13801 gctgcttgcc ggaggacaga atgcgagcgt tgcggccgac cacttcggac ttgcgatagc
    13861 cggtaatgcg cgaaaacgcc gggttgacgg tctcgatgac gccgttggcg tcggtgatca
    13921 tgatgccttc cagggtggat tcaaacacct tgtcggccat gcgcaggttt tgccgggaaa
    13981 tctgcagcgc ttcgtcgcgc tctttcagcg cgtattgcag ctcattgacg tattcgtgct
    14041 cgatgctggt gaggatgtca gaaaaactga tcacgccaac cagttcgccg cggtccagca
    14101 cgcccaggtg gcggatgtgt ttttccgcca gcagcttgcg cgcgtaatac aggctggttt
    14161 gcttgggcac actgatgatg ggatggctgg ccaccgcgcc caccggcagg tgggaggttt
    14221 tttccgccag cagacgcacc acgtcgcgct gggtgagaat gccgtaatca ctgtccgggt
    14281 agcacaccag cagcgcttcg acatgctgtt cgcgcatcag gctgatggcc tcgtgcagcg
    14341 gcgtatcggc cggggtcatc agcgggcggc gctcggggtc gaggatggtg tccaggtgct
    14401 tgagtttgag aaagaactca gcgccctaat tcagcacgat atcgctttgc gacaggatgc
    14461 cgcgataacc gccctgatcg tccaccacca gataatggcg cacgccatgg cgcttgaaca
    14521 acacggcggc atcgttgaag gtggtgtcta ccgagatggt tttcaccggc cagctcatca
    14581 cgtcgcgcac cgacagctca aaggtggcgg gatcagcaaa attcagccgc aaggcgtcgg
    14641 tttcggtcca gatgcctacc gcgcgggtgt cttcgatcac cagcagcgag ccgcaggcgg
    14701 cctcacgcat caagcgggca gcctcaacca cactggtttc aggctggcag gaaaaaatgc
    14761 gcggggaaat gatttgctcg atggtgcgac ggacgggcac tgcaggaagc tctccaagtg
    14821 aggcgtcggt gaacggctca agaataggga tggtcagatc aagcgacacg gcggcgtcct
    14881 gcccaggatg tctgctgtcg tgctgccatg ccacgctgtc attccagcct ccgttgcgcg
    14941 gtgcgcgcca agccaatatg cctcaatctt atcttggcat aattgcctgt ttgacccgca
    15001 ctgtcaaaaa atactgtctt gcagcgcagc atggtttcat gcgtttgatt caccgtcaag
    15061 cacacagtct gacaataaaa aaccctgttc cgactggcgg aacagggttt ttgctacaca
    15121 ccttgcaggg gacaggctca ggcgcgttcg accgccaggg caacgcccat gccgccgccg
    15181 atgcacagcg tagccaggcc cttcttggcg tcacggcgct gcatttcgtg cagcagggtc
    15241 accagcacgc ggcagccaga cgcgccaatc gggtggccca gagcaatggc gccgccgttg
    15301 acgttgacct tgttcaggtc ccacttcagt tcacgggtca cgcccagcgc ttgcgcggca
    15361 aaggcttcgt tggcttccac cagatccagg tcttccaccg cccagccagc cttggccagg
    15421 gctttttgcg aggcgctgac cgggcccatg cccatgattt ccggggcgca gcccgacagc
    15481 gcgtagccag ccacgcgggc catcggggtc aggcccagct cggcggcctt ggcggcgctc
    15541 atcatcagca cggcggcagc gccgtcgttg atgcccgagg cgttgccggc ggtgacggtg
    15601 ccgtccttct tgaaggcggc gcgcagcttg cccaggcctt ccagggtggc gccgtgcttg
    15661 atgaattcat cgttgacgaa ggcaaccggg tcgcccttgc gctgcggaat cagcaccggg
    15721 gtgatttcgt cggcaaactt gccggccttc tgggcggctt cggcgcggtt ttgcgaggtg
    15781 acggccagtt ggtcttgctc gtcgcggctg atttcatact tggcggcgat gttttcggcg
    15841 gtgatgccca tgtggtactg gttgtaggcg tcggtcaggc cgtcgtacac catggtgtcc
    15901 accagttggg cattgcccat gcggaagccg tcgcgcgagc ccggcaggat gtgcggggac
    15961 atcgacatgt tttcctgacc gccggcaatc acgatttccg cgtcgccatt ggcgatggct
    16021 tgcgccgcca ggtgggtggc gcgcaggccg gagccacaca ccacgttcag ggtggaagcc
    16081 ggggtggtca ccggcaggcc agccttgatc agcgcctgac gggccgggtt ctggccaacg
    16141 ccagcggtca gcacctgacc gaggatcact tcgctgaccg cttccggggc cacgccggtt
    16201 ttttccagca gggacttgat cacgatggcg cccagatccg gggcgctgat tttggccagg
    16261 ctgccgccaa acgagccgat ggcggtgcgg gttgcagcga cgatgactac ttcttgcatg
    16321 actttctcca gaaacagggg tcgcgccggc caaaggccgg cgcatgatgc gggaaaacag
    16381 cgatgtcgcc tgggcttagg ccatcatggc ggcggtttgc ggcatggagc gggccagcac
    16441 aaaggtgccc ggcgcggcgc acagcggctt gaagtccttg ttgcccgggg tcttgggcgc
    16501 agccacctga gcgccagagc gcggggcgag ccattgatcc cagtcttgcc accagctgcc
    16561 cggacgcgat tcggcgccag ccagccactc ttgggcggtg gccgggcgat tgtcgttcac
    16621 ccagtagttg cgcttgttct tggtcaccgg gttgatggcg ccggcgatgt ggccggaggc
    16681 gcccagcaca tagcgcttgt ccttggcgcc ggtcaggtat ttcaggccat cgtaagccga
    16741 ggtccacggc acgatgtggt cttcgcgggc ggcaaagatg tacagcggca gctcgatggt
    16801 ggacacatca atcggcacgc cgcacagggt gatggcgccc ttcttggtca gggcgttgtt
    16861 caggtagaac tccttcagga agaaggtatg catcggcagc ggcaggtcca ccgagtcgtt
    16921 gttccagtac aagaggtcaa acggcggcgg ggttttgccc agcaggtagt tgttgaccac
    16981 gtagttccac accaggtcat tggcgcgcag actggcgaag gtctggccga tttccttgcc
    17041 agagacgatg ccaccagcgg ccaccttggt ttcgcggctg cggatcaggt tttcgtcgat
    17101 gtacatgcgg atctcgcccg gatcggtgtg atccaccagc gaggtcatga aggtggccga
    17161 ttccacccag tccaggccac ggtccttgag cacacacagc gtggtgttga ggatcacgcc
    17221 gccgatgcag aagcccagga cattgatctt gtcctgcttg ctgatcttgc gcacggtgtc
    17281 gatggcggcg atggcgccct tgtcgatgta ggtgtcccag gtgaagtgct tcatctcgcg
    17341 gtcggcggat ttccacgaca ccagatacac ggtgtaaccc tggctgacga agtggcgcac
    17401 catggagtta tccgggccca gatccatcag gtagtacttg ttcacgcacg gcggaatgat
    17461 cagcagcggg cgttcgtaca ctttttcggt ggtcggggtg tactggatca gctcgatcag
    17521 ctcattgcgg aagatcaccg agcccggggt ggcggcgatg ttcttgccga tctcgaactt
    17581 ggattcgtca gacatggaaa tgtagccctt ttccatgtcg gccatcatgt gcttcatgcc
    17641 ttcgaccagg ctctcaccct tggtttcgat ggcgcgcttg agcacttccg ggttggtcat
    17701 ggcaaagttg gtcgggctca gcgcatccag atactgcttg gtgaagaagc tcatgcgctc
    17761 cttctgatgg gcgtcgatag gcacgccgtc gaccatttcg gtcagccatt tggaggcaaa
    17821 caggtaattt tgcttgagca ggttgaagaa gggatggtca ttccactcgg cggcggcaaa
    17881 acgcttgtcg gacggatccg ggctggcgac ttccggcact ttctggccca ggaagttcat
    17941 ccacagattc atctgctgct ggtacagatt ggtctgcatt tccaccagtt g
//
LOCUS       DAOPYX010000022        17677 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_3362_length_17677_cov_8.053796, whole genome shotgun sequence.
ACCESSION   DAOPYX010000022 DAOPYX010000000
VERSION     DAOPYX010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 17677)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17677)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17677
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3362_length_17677_cov_8.053796"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..578)
                     /locus_tag="PKN55_02220"
     CDS             complement(<1..578)
                     /locus_tag="PKN55_02220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012697695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HNX70091.1"
                     /translation="MTISRRTLLVSALLSAVMLSACGKKEAAPAPAAEAPAAPQVKEY
                     VVGTDAAYAPFEFENEKKEVEGFDMDVLRAVADKAGFKVKFVNTPWEGIFATLAQGDR
                     DMVISAVTITDERKQSMDFSDPYFEARQLIAIGKGVTDVKKFADLKGKKVAVQTGTTG
                     DEVVQKLLGKTSADIKRFESTPLALKELESGGV"
     gene            complement(657..1298)
                     /locus_tag="PKN55_02225"
     CDS             complement(657..1298)
                     /locus_tag="PKN55_02225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX70092.1"
                     /translation="MLAHFARLCTVFVRCLACLVGGFLLADLAFVVPRLAPVLCRMFS
                     VFLVCHGFLACVSDAGLACTVCMLSALAGYGFACVMTTMSHGFALAALVCPLAKMLAG
                     MALAMAGDTGMRFAGLLGTCLAADRCGIAWQWQIIGQREAGQQCQHAGKQNAKHEYNP
                     FLKHMAILHCIITMCHKLEKNVLNSPLCGVKFPEYPLKQHSALGYYGKSNTLV"
     gene            complement(1575..2411)
                     /locus_tag="PKN55_02230"
     CDS             complement(1575..2411)
                     /locus_tag="PKN55_02230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX70093.1"
                     /translation="MTSQDNHSRTQAPSHVSGRQARKSSRWLGRLMALAVLVVWPLVL
                     VGFFISSCGEEREFFDEPLMRQLGARPDRITSYDFHGLMVPGTKEDAKRLGFSDCSAN
                     DAFFYCTRPRMGKFLGVEVRAASLILDEMDNFIETRFSIQKRNPGVFSYRSIQIEFGP
                     TFYDTDCLNKHNKERPGHAWVRLLECRKNDGIDYFKYLLQADGWVNDTSGRGRVDAYY
                     KRGIPLEIEVGVVNGTANIRPFPLKDVELITQAQAQAASTPAPHDDSQASREKFVRSM
                     KQ"
     gene            complement(2592..4139)
                     /locus_tag="PKN55_02235"
     CDS             complement(2592..4139)
                     /locus_tag="PKN55_02235"
                     /inference="COORDINATES: protein motif:HMM:NF013155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX70094.1"
                     /translation="MKNSLTTALSAVLLLLAALPAMALDDRAASAQVNRWLQAHRQQI
                     PLLRQFVQRLPKGADLHSHLTGAAYAETYLRLAAEHGLCVDSQTLAFVSPDGATCPSP
                     SVSARQLPSGVYSAMVNQLSIRNVALSGSSGHDQFFATFSRFRLPANLPEASVALAAE
                     VSNRAASQNILHLELMTTLQGSGVRELAGKLPWPQTPDFAASLAWLQANGLDAQVEAA
                     RNELDQFDQAYRATQRCGQPDAQPGCQISLRWQQQTSRTASPAQVFSQLAFAFALAHA
                     DGRVVGINLVAPEDDPVALRDYRLHMAMIGWLSRQMPDVNIALHAGELTQGLTPPETL
                     RHHIADAVNVAGAKRIGHGVAIGYEDHAARTLATMHTRQVAVEVCLSSNDVILGVSGE
                     QHPLPVYLAAGVPVVLASDDEGVSRIDLSHEYLRAAQTYRLSYPQLKQLSRNSLEYSF
                     LPGDSLWRNLSAGKVLAVCRNDTPGAKALSPRCAHALGSSEKAQRQWQLEAAFRQFER
                     DPLWAGR"
     gene            complement(4798..5037)
                     /locus_tag="PKN55_02240"
     CDS             complement(4798..5037)
                     /locus_tag="PKN55_02240"
                     /inference="COORDINATES: protein motif:HMM:NF017690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNX70095.1"
                     /translation="MECGVWACEPGMWRIRFAANKLEFFCVLEGHVRLHDADGQVHDV
                     RAGEAGVIPAGFVGAFEVLSPVRKYFVVQQVSISA"
     gene            complement(5184..5600)
                     /locus_tag="PKN55_02245"
     CDS             complement(5184..5600)
                     /locus_tag="PKN55_02245"
                     /inference="COORDINATES: protein motif:HMM:NF036804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family metal-binding protein"
                     /protein_id="HNX70096.1"
                     /translation="MTGHRCKTPATPCPCGSGLALAACCGRLHAGELAVSAEALMRSR
                     YSAYVLGNEAYLLATWHPDTRPAVLGLADDAPVKWLGLDVLACQAGQPGDAQGTVRFV
                     ARYKVNGRAQRLCEHSRFICQDGRWLYVDGQVDEAH"
     gene            complement(5581..6018)
                     /locus_tag="PKN55_02250"
     CDS             complement(5581..6018)
                     /locus_tag="PKN55_02250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX70097.1"
                     /translation="MKSTLLAGACALVLLSACSAVQAPPPVAPPAPDFNAMQNSLFQE
                     TKRLANLVGNGSLSRVEAADQLNARRLAMIGPHPVDDEVFRTYRLLSVQVQQNKITQD
                     KLRITLMNKLDAVRKGYALLDPKPVKPPVFTNMLLQIYDRPPL"
     gene            complement(6243..8126)
                     /gene="speA"
                     /locus_tag="PKN55_02255"
     CDS             complement(6243..8126)
                     /gene="speA"
                     /locus_tag="PKN55_02255"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008953808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HNX70098.1"
                     /translation="MAWTIADSRSMYGIRHWGAGYFDIGDNGQVCVHPRGKHDDASLD
                     LYQLTRDLRARGLALPLLVRFPDILQHRVRRIIGGFDQAREKLGYENGYTLLYPIKVN
                     QQEAVIKSMIADPTLAIGLEAGSKPELMAVLALAPSGGTIVCNGYKDREFVRLALIGQ
                     KLGHKIFIVIEKEAEVPLVIEESKRLGLTPMVGLRVRLSALSHGQWADSGGEKGKFGL
                     SAAQVLSVIGRFVDAGMQNAIRLMHFHMGSQIANISDYRLGFREAVRWYGELINMGLP
                     VDHVDVGGGLAVDYDGTHSRNPWSVNYTIGEYAETIVGMMHEFCDAHGLPMPHLLSES
                     GRALTAHHAVLITNVTDVEQPIDAVPSVDTLEELADPLHKLYELALTGDIELAAETYY
                     RAGQYVATVTELYTDGKLSLAEKAFAEQCYAALCRRLHRALTAAQRSHRQVYDELHDK
                     LADKYFCNFSVFQSLPDTWGIGQLLPTVPLHRLDEQPLRRAVLQDLTCDSDGKVTQYV
                     DHQSIESSMPVHDLKPDEEYLIGVFLVGAYQEILGDMHNLFGDTDSVNVYTQDDGSLR
                     IEGVEVHDTIEDMLRYVHLQPEDLISRYLDKIVRAELTSDERGAFLNELKQGLKRSSY
                     LTV"
     gene            complement(9051..9173)
                     /locus_tag="PKN55_02260"
     CDS             complement(9051..9173)
                     /locus_tag="PKN55_02260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012697186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3149 domain-containing protein"
                     /protein_id="HNX70099.1"
                     /translation="MALMKELLSSDVGILSLITIVVTCVVVGTAITFFIKKSHE"
     gene            9398..9757
                     /locus_tag="PKN55_02265"
     CDS             9398..9757
                     /locus_tag="PKN55_02265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX70100.1"
                     /translation="MSLDLSPTPAQEHEARKWVIIVYVLQILSLFIGITSIIGIIINY
                     IKLDDVRGTWLESHFRWQMRTFWFSLLWAVLGGLTFWFGVGIVVWIVAGIWGLYRIIK
                     GAIYLYDGKTMYMNTLP"
     gene            10244..11947
                     /gene="ilvB"
                     /locus_tag="PKN55_02270"
     CDS             10244..11947
                     /gene="ilvB"
                     /locus_tag="PKN55_02270"
                     /inference="COORDINATES: protein motif:HMM:NF006016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HNX70101.1"
                     /translation="MHASSNTAAHGEAEWLTGAELTVRLLERQGVRVLSGIPGGAILP
                     FYDALSASTEIHHVLARHEQGAGFIAQGMARTTGQAAVCLASSGPGATNLLTAIADAK
                     LDSIPLVAITGQVPQSMIGTDAFQEVDTYGLSVPITKHNFLVDSAEALLEVIPRAFSL
                     ALSGRPGPVLVDIPKDVQLQRIAVSHWPEPGCAQAAPAPDADKIAQAAQMINQAKRPI
                     LYLGGGVVHSGASEAAVTLAEQASLPTTMTLMALGAMPVDHPLSLGMLGMHGAPYTNL
                     ALEECDLLIAVGARFDDRATGKVAAFCPNAAIIHIDIDPSELDKIKTAHVGIAGDVGA
                     VLAQLLPQVQASLREDWNQRVAQLKAERPLQLPGLDDPRSHYGLVQAVADALDDSAFV
                     ATDVGQHQMWVAQAYPLRRPRQWLTSGGLGTMGFGLPTAIGAALANPERTVVCFSGDG
                     SILMNIQEFATLAEAGLNVKVVLMNNASLGLVHQQQELFYGERIFASRFIAQPDFLQI
                     ARGFRIAELDLDAAADPRAALKQALNAPGPCLIHASIDVKEKVYPMVPPGAANKEMIG
                     G"
     gene            11950..12240
                     /gene="ilvN"
                     /locus_tag="PKN55_02275"
     CDS             11950..12240
                     /gene="ilvN"
                     /locus_tag="PKN55_02275"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF006036.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HNX70102.1"
                     /translation="MTAEQLTRPVLEIDVNNHPGVMSHVVGLFSRRAYNVEGIICVPV
                     GTGETSRIWLQINEDERLEQIVKQVEKLEDVLVVRRHDAGHPAFAGLERYAG"
     gene            complement(12330..13454)
                     /locus_tag="PKN55_02280"
     CDS             complement(12330..13454)
                     /locus_tag="PKN55_02280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014087205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HNX70103.1"
                     /translation="MTAKVNPQHKHKKALLIGGGAPNSTLMAGALVAFLEKGAEFDVI
                     SASGAGALMGLLYAAPKNGNPRQALEDWAKVGVSDAIYNRFPVNFKVFMKPGEQAAAY
                     RDWIMNRNPFTKPLYDPSSPMGHGFFSDMAKLFLATLSPSDLSGKSLGMCAHLPFAEQ
                     VIDFDAIPKLKPEFYINAYNMNRAEMTIWGKGDITPAHLRAAFSFPLIYPPYTLNGED
                     YIEGAAIDTLNFKALVNDNEDAKQKGLHCDLDTLVIFDILGADQLIRKPRDLYDAWVG
                     SIITPLVEIAKDDISLFQYRHNIDHKTGKEKRKLLKVDLMGGIPKEHWPQVMDWSASN
                     LELLFKVGYEAGLKFCDENAELLDLPQAKAKQAAPAEAAV"
     gene            complement(13670..14788)
                     /locus_tag="PKN55_02285"
     CDS             complement(13670..14788)
                     /locus_tag="PKN55_02285"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HNX70104.1"
                     /translation="MNSTPHVKTGLVLGGGAPNSSLIAGALVAFAEREMEFDVMSTSG
                     AGALMGLLYNCPRGGDRIEALTRWSQIGVSDELYQFFPVNHKVFMKPGDHAHQFRQSM
                     ASNPWMKWCQSMFPQGGLLSDMMQLTWASMCPSNLSKKSLGLCAHLPFVEDYVDFDLA
                     HHHAGEFYFNAFNLDESRMEVWGKEKLSAERIRAAFSFPFIYPPYELDGQWYIEGASI
                     EPLNFEVMTRPEVEDHVDVVIVADILGSYRLLQKPRNLYDAWVKSIITPLVKIAHSDT
                     QMYKMQMAQHGKTVLQLDFYKNVPDAWLPEIFDWSESNLKRLFEVGYQTGQEFLEEHG
                     LLLGLLPATGKPARVHHAAVRQNHHTLPDLRMPAGALI"
     gene            15703..16017
                     /locus_tag="PKN55_02290"
     CDS             15703..16017
                     /locus_tag="PKN55_02290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008954011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HNX70105.1"
                     /translation="MEQFDNVSVIKKANVYFDGRCVSHSLILADGVRKSVGVILPGAT
                     LTFNTGAPEIMEVLAGQCSVQLKGESTAQNYGAGQSFSVPGDSAFDITVTDTLHYVCH
                     FG"
     gene            complement(16183..16542)
                     /locus_tag="PKN55_02295"
     CDS             complement(16183..16542)
                     /locus_tag="PKN55_02295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009311688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HNX70106.1"
                     /translation="MSTPVLYGIPNCDTMKKARAWLAAQGIEVTFHDYKKLGITAEVI
                     EGWLAQGATLDALVNRKGTTWRKLDEASRAQVEHREGAIALLLAQPSLIKRPVLVQAG
                     RVEVGFSEAAYQALFAH"
     gene            complement(16652..>17677)
                     /locus_tag="PKN55_02300"
     CDS             complement(16652..>17677)
                     /locus_tag="PKN55_02300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008954488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNX70107.1"
                     /translation="KGAMLLHRNIVANMLQAGAWVKPVVREGQEVIVTALPLYHIFSL
                     TANLMVFTEIGALNILITNPRDIPGFIKEIKQYPVTAMTGVNTLFNALLNHPDFASVN
                     FSTWRLALGGGMAVQKAVADKWKAVTGVPLIEAYGLTETSPAACMNPLDLTEYNGTIG
                     VPIPSTEIQIRDSQGHEVAYGESGELCIRGPQVMKGYWRRPDETAKVIDEQGFLATGD
                     MAMMTPEGFVKLVDRKKDMILVSGFNVYPNEVEDVVASHPGVLEVACVGVADEKSGEV
                     VKVFVVRKDPALTEKDILAHCKANLTGYKVPKFVEFRDELPKTNVGKILRRALRDEKP
                     GMTANKG"
BASE COUNT         3883 a         5317 c         5019 g         3458 t
ORIGIN      
        1 acgccgccgc tttccagctc tttgagcgcc agcggggtgg attcaaagcg cttgatgtcg
       61 gcgctggtct tgcccagcag cttttgcacc acttcgtcgc cggtggtgcc ggtctgcacg
      121 gcaactttct tgcccttcag gtcggcgaat ttcttgacgt cggtcacgcc cttgccgatg
      181 gcgatcaact ggcgggcttc aaagtacggg tcagagaaat ccatgctttg cttgcgttcg
      241 tcggtgatgg taacggcaga gatcaccata tcgcggtcgc cttgggccag ggtggcgaaa
      301 atgccttccc acggggtgtt gacgaacttg accttgaagc ccgctttgtc ggcgacggcg
      361 cgcagcacgt ccatatcaaa gccttcgact tcttttttct cgttttcgaa ttcaaacggg
      421 gcataggcgg catcggtgcc aaccacatat tctttcacct gcggggccgc cggtgcttca
      481 gctgccggag ccggggcggc ttcttttttg ccacacgcgg acaacatcac ggcgctcaag
      541 agcgcgctca ccagcagggt gcgacgggaa atggtcataa ttcgggaacc tcgtgataaa
      601 gtaacggacc tagaggggcg acgctcatca ggcacaatgg tgccgagcga tcgcaatcaa
      661 acgagagtat tactttttcc atagtaccca agagcagaat gttgcttcaa tgggtattct
      721 ggaaatttta cgccgcaaag cggcgaattc agcacgtttt tctccaattt gtgacacatg
      781 gtgatgatgc aatgcaatat cgccatgtgt tttaagaaag ggttgtattc atgcttcgca
      841 ttttgcttgc ctgcatgctg gcactgctga ccagcctcac gctggccgat gatctgccat
      901 tgccaggcga tgccgcaccg gtcagcagca aggcacgtgc ccaaaagccc agcaaacctc
      961 atgccagtgt cgccagccat ggcaagcgcc atgccggcaa gcatcttggc aagcgggcac
     1021 acaagcgcgg caagggcaaa accgtgactc atggtcgtca tcacgcaagc aaagccgtat
     1081 ccagcaaggg cagacagcat gcacacagtg cacgcaaggc cagcatccga cacgcaggca
     1141 agaaagccgt ggcacaccag aaacacacta aacatgcggc aaagcacggg cgcaaggcgg
     1201 ggcaccacaa aggcaaggtc agccagcaga aaaccaccca caaggcaggc aaggcagcgc
     1261 acaaacacgg tgcacagtcg cgcaaagtga gcaagcatgc aggtaaacct gcacgctctg
     1321 cacgcgccac gcatccaaag aaacaggcac gtcaccgcac acatcacaga taagggagtc
     1381 ggcgcgttga catgcaccat ggcgcacttg tgcgtttggt gcatgtcatt gagcgcagag
     1441 cacaaagccc ggattagcac gtccgggcga tgccaagaag gtcagacaga agcaaccacc
     1501 tcagcagatc atgtcacgcc atatagctct ggccagagcc tcataaccac tttgttgagt
     1561 atggtgagtg gctcttattg cttcattgag cggacaaatt tttcgcggga agcctgacta
     1621 tcatcatgag gcgccggcgt agatgcagcc tgagcctggg cttgtgtgat cagctcaaca
     1681 tctttaagtg gaaatggtcg gatattggcc gtgccgttga cgactccgac ttcaatctct
     1741 aaagggatac cgcgcttgta atacgcgtca acccgaccac gcccactggt atcatttacc
     1801 cagccatcag cctgcagcag atatttaaaa taatctatgc catcattttt acggcattcc
     1861 aacaatcgta cccatgcatg cccgggtcgc tctttgttat gtttattcag gcagtccgta
     1921 tcataaaaag tcgggccgaa ttcaatttga atcgaacgat aactaaatac gccgggatta
     1981 cgcttctgga tactgaaccg agtttcaata aaattgtcca tctcatccag aatcaacgat
     2041 gccgcacgca cctcaacccc taaaaacttg cccatccgcg gccgggtgca ataaaaaaag
     2101 gcatcattgg cactgcagtc agagaagcct agcctcttgg catcttcttt ggtgccaggc
     2161 accatcaggc cgtgaaaatc gtaggaagtg attcgatctg gtctggcacc cagttggcgc
     2221 atcagtggct catcaaaaaa ctcgcgctct tctccgcatg agctgatgaa aaaaccaacc
     2281 agaaccagcg gccacaccac cagcacggca agcgccatca ggcgccccag ccagcgagaa
     2341 cttttgcgcg cctgcctccc ggaaacatga gaaggagctt gcgtgcggga atgattgtcc
     2401 tgactcgtca tttgttgctg tacctgtgtc cacccgccat atcaggcgta agacaatcac
     2461 ccgggagaaa cctgtttacc agagcttggg caataccgat gtgcctgctc ggcatcgccc
     2521 atcaacatgc gccaaacatg ccaaaaatat caaaaagcaa caaagcggca aattttctgc
     2581 acatcagcag attaccgacc agcccacagc gggtctcgtt caaactgtcg gaatgcagct
     2641 tccagttgcc actggcgctg ggctttttcg ctactgccca gtgcatgggc gcaacgcggc
     2701 gacaaggcct tggcgccagg tgtgtcattg cggcatacgg ccagcacttt acctgcagac
     2761 agattgcgcc acaggctgtc accaggcaga aagctgtatt ccaggctgtt tctggataat
     2821 tgcttgagct gtggatagct cagccggtaa gtttgtgccg cgcgcagata ttcgtggctc
     2881 agatcgatgc gggatacacc ctcatcgtcc gaggccaata ccaccggcac accggcagcc
     2941 agatacactg gcagaggatg ctgttcaccc gatacaccca gaatgacgtc attgctgctc
     3001 aaacacacct cgactgccac ctgacgagtg tgcatggtgg ccaaggtgcg ggcagcatgg
     3061 tcttcatagc caatcgccac accatggcca atgcgcttgg cgcccgcaac gttcaccgcg
     3121 tcagcaatat gatggcgcag ggtttccggt ggcgtcaggc cttgcgtcaa ttcgccggca
     3181 tgcagggcga tattcacgtc cggcatctgg cgcgacagcc agccgatcat cgccatatgc
     3241 aggcgatagt cgcgcaaggc cactgggtca tcctcaggcg ccaccagatt gatgccaacc
     3301 acacggccgt cggcgtgggc cagggcaaag gcaaaagcca attggctgaa cacctgtgcc
     3361 gggctggcgg tgcggctggt ttgttgctgc cagcgcaggc tgatctggca acccggctgg
     3421 gcatcaggct gcccgcaccg ctgggtagcg cgataggctt gatcaaactg atccagttca
     3481 ttccgggcag cctcgacctg agcgtccaga ccattggcct gcaaccaggc caggctggcg
     3541 gcaaagtcag gtgtctgcgg ccagggtagt ttgccggcca actcgcgcac ccccgaacct
     3601 tgcagagtgg tcatcagttc cagatgcagg atattctggc tggccgcccg attgctgact
     3661 tcggcagcca gggccaccga ggcttcaggc aagttggcgg gtaggcggaa gcgggaaaaa
     3721 gtggcaaaaa actggtcatg cccactgctg ccggacaaag cgacattgcg gatggacagc
     3781 tggttgacca tggcgctgta tacgccggac ggcagttggc gggcagagac cgacggcgac
     3841 gggcacgtcg ccccatctgg agagacaaat gccaacgttt ggctatcgac gcacaagccg
     3901 tgctcggcgg ccaggcgcaa ataggtttcc gcatacgcgg cgccagtcaa atggctgtgc
     3961 agatcggccc ccttgggcag gcgctggaca aattgtctca gcaaagggat ctgctggcgg
     4021 tgggcctgca accatcggtt gacttgcgca ctggcggcac gatcatccag cgccatggcg
     4081 ggcaaggccg ccaagagcag cagcacagcg gacagggcgg ttgtcaggga atttttcatg
     4141 tctggatctg ttaaacggca gtcggaggcg tgttaggtca gtcaggccaa tgcaaagggg
     4201 catgaaccgt catgcaggtc atggcgaaaa tacagcctat gtcaaatgct tgcatggaca
     4261 tggatatata catgacccac aggctcctga aaatcaaaca ccatcaatat ggccaatcgg
     4321 cgcatgcccg ccacggcccc atctggccca gtatttttac ttaataaatg gcatgccaac
     4381 atgcctaccg agcattgcag gcatgatata ccagtttaag cccgatgttg gccatgatcc
     4441 aacgcaagcc aatcgatggc tacgtatttc tacctaataa ataaatagct gtaacatggt
     4501 aaaaaacgga tggcggggga ttaaacgaca gaatgccttg gcttgcaaga ggcaggtcgt
     4561 tatcagaaga tagaatcgta cttaaaaacc cagcgcagca ttcctgacca ggcgcgcagc
     4621 cgcagatagc gcaaggagta cggcaaagcc gtgaatgaca ccaggcgggt tttgccagca
     4681 acgcttaggc ggagacgacc agcaagccgg cagcgccgcc ctggacaaac cgcgagagcc
     4741 aattaccagc gcggccaaca cgctgcatga cttccacctc atcgcacgat ggcaaaatca
     4801 agcagatatc gagacctgct gcaccacaaa atacttgcgc accgggctga gcacttcaaa
     4861 cgcccccaca aacccagccg gaatcacccc ggcttcaccg gcgcgcacgt cgtgcacctg
     4921 accatcagca tcatgcagac ggacatggcc ttccagcaca caaaaaaatt ccagtttgtt
     4981 ggcggcaaaa cggatacgcc acataccggg ctcgcacgcc cagacaccac actccatacc
     5041 gtgcatggca tcagtaaaat aggtccaggt ctgccgttgc ggcgcaccat gcacccgacg
     5101 ctctgcggcg ggctggtcat gaacggggac gggggcgtgt tcggcaaatg ccacaaaacg
     5161 caagagatca ggcgtaacca tcatcaatgg gcttcatcta cttggccatc cacatacagc
     5221 cagcggccat cctggcaaat aaaccggctg tgctcgcaca ggcgctgagc acggccattg
     5281 actttataac gggccacaaa gcgcaccgtg ccttgtgcgt cgcctggctg cccggcctgg
     5341 caggccaaca catccagccc cagccacttg accggcgcat cgtctgccag acccagcacg
     5401 gcaggtcggg tatccggatg ccaggtggcc agcagatagg cctcattacc cagcacataa
     5461 gcgctataac gtgagcgcat caacgcctcg gcactgaccg ccagctcacc agcatgcagg
     5521 cggccgcagc aggctgccag cgccaggcca gaaccacacg gacatggcgt ggcaggcgtc
     5581 ttacaacggt ggccggtcat agatttgcag caacatattg gtgaacaccg gtggcttgac
     5641 cggcttgggg tcgagcaaag cataaccttt gcgcaccgcg tccagcttgt tcatcagggt
     5701 gatgcgcagc ttatcctggg tgatcttgtt ctgttgtacc tgcacactga gcagtcgata
     5761 ggtgcggaac acttcgtcat ccaccggatg cgggccaatc atcgccagtc ggcgggcatt
     5821 caactggtca gcggcttcaa cccggctcaa gctgccattg ccaaccagat tggccaggcg
     5881 tttggtttcc tgaaacaggc tgttctgcat ggcgttgaag tccggcgcag gcggcgcaac
     5941 cggaggcggc gcctgcacag cggagcaggc ggacaacagc accagtgcac aagcaccggc
     6001 aagcagggtg gatttcatcg ggcgtctccc tcgaaatgat tctgctgatg gcatgatttt
     6061 gcgcaagtat gccgcaaaat cgcatggttt gtccgctatc cgggcatgcc acgagctttt
     6121 tcccggcccc gacgctcagg catgacaggg ccggagagaa acggcagcca gggtcaccgg
     6181 gcatcactcc cggcatctga ctacgggcag agcagcccta caccgggaga cgaaggccga
     6241 agttacactg tcagataaga agaccgcttc agcccctgct tgagctcgtt caggaaggcg
     6301 ccacgctcat cgctggtgag ctcggcacgc acgattttgt ccagataacg gctgatcagg
     6361 tcttccggct gcaaatgcac ataacgcagc atgtcttcga tggtgtcgtg tacttccaca
     6421 ccttcgatgc gcagcgaacc atcatcctgg gtgtagacgt tgactgaatc cgtgtcgcca
     6481 aacagattgt gcatatcgcc cagaatttcc tggtaggcgc ccaccaggaa cacgccgatc
     6541 aggtattcct cgtccggctt caggtcgtgc accggcatcg atgattcaat gctttggtga
     6601 tcgacatact gcgtcacttt gccgtccgaa tcgcaggtca ggtcttgcag cacggcgcgg
     6661 cgcagcggct gctcgtccag ccggtgcaaa ggcacggtgg gcagcagctg gccaatgccc
     6721 caggtgtccg gcagcgactg gaacaccgag aaattgcaga aatacttgtc cgccagcttg
     6781 tcgtgcagct catcgtacac ctggcggtgc gaacgctggg cggcggtcag cgcgcggtgc
     6841 aggcggcggc acagcgcggc gtagcattgc tcggcaaagg ctttttccgc caggctcagc
     6901 ttgccgtcgg tgtacagctc ggtgacggtg gccacatact ggccggcgcg gtaataggtt
     6961 tctgccgcca gttcgatatc gccggtcagc gccagctcgt acagcttgtg cagcgggtcg
     7021 gccagctctt ccagcgtgtc cacgctgggc acggcgtcaa tcggctgctc gacgtcggtg
     7081 acattggtga tcagcacggc gtggtgcgcg gtcagcgccc ggccggactc ggacagcaaa
     7141 tgcggcatcg gcaggccgtg ggcgtcgcag aattcgtgca tcatgccgac gatggtttcc
     7201 gcgtattcgc caatggtgta gttcaccgac cacgggttgc gcgaatgggt gccgtcgtaa
     7261 tccaccgcca ggccgccgcc cacgtccaca tggtccaccg gcaagcccat attgatcagc
     7321 tcgccatacc agcgcaccgc ctcgcgaaaa cccagccgat agtcgctgat gttggcgatt
     7381 tgcgaaccca tatgaaaatg catcagacgg atggcgtttt gcatgccggc atcaacaaaa
     7441 cggccaatca ccgacagcac ctgcgccgcc gacaggccaa acttgccttt ttcgccgccg
     7501 ctgtccgccc attggccgtg cgacagcgcc gacaggcgca cgcgcaggcc caccatcggc
     7561 gtcagcccca ggcgcttgga ttcttcgatc accagcggca cttctgcttc tttttcgatg
     7621 acgatgaaaa tcttgtggcc cagcttctgg ccaatcagcg ccaggcggac aaattcacgg
     7681 tctttatagc cattgcagac aatggtgccg cccgacggcg ccaaggccag caccgccatc
     7741 aactcgggct tggagcccgc ttccaggcca atggccaggg tcgggtcggc gatcatgctt
     7801 ttgatcaccg cttcttgctg gttgaccttg atcgggtaga gcagggtgta gccgttttcg
     7861 tagccaagtt tttcccgcgc ctgatcaaag ccgccgatga tgcggcgcac ccgatgttgc
     7921 agaatgtcag gaaagcgcac cagcaagggt agcgccaggc cgcgcgcgcg caggtcgcgg
     7981 gtgagctggt agagatccag cgaggcgtcg tcgtgcttgc cgcgtgggtg cacgcacacc
     8041 tggccgttgt cgccgatatc aaaatacccg gcgccccaat ggcgaatgcc gtacatactg
     8101 cggctgtctg caatggtcca agccatgaat agagtccttt cgtggggccg cagggcggcc
     8161 gcgtcgattg atccagtcac acagcgcccg cttcacggct tggggccgaa tcgaggcgaa
     8221 aggctcggcc gcgcggcatg cggccggcgg acctggcggg cgcgaaaaaa ggacgcggat
     8281 tgtagcaata aatacttggc gcgcggggaa tttccctccc tcaccaagcc aggaagccac
     8341 cggcaaaaac aatggcatcg ccccttcttc cgccagcaca acttacggaa gctgagtgag
     8401 agcggccaac aaaactcagc acagcggtcc ttggccggcg tgtggccgta cacagtacgg
     8461 gtgtgcggca aggccacaca acaacgccag gagagttttg ccagccagtc ttgatctgca
     8521 ccatagcaga gcttgatgac tgtttgacgg tatggaaacg acgcgtggca gacaggcaca
     8581 gcatctgagc gcaagagaca agaatcctta cagacatagc cgataacatg catcgtcata
     8641 tgacaagcaa agtattttac taaatcaggg agaatgcgcg catcacccaa aagctttcct
     8701 gaagcaagac ccagaaaact ggttttattg catacacagt acatccatca agccaggaaa
     8761 atcagcgcac caattcagca gattttttgg cccagcgcga gtcgttccat gcatctgaca
     8821 aaggtactgt attgacacgc cacagatccc tcatcaccca cgcggcacaa ggcagatcgt
     8881 gcagcacaca cagaacgcca cagccagaaa acaatcaaca tactgattct taaaaaagaa
     8941 aacggcccga cgatgcaaga catcgacggg ccgttgtccg tcatcccccc ggacgacagg
     9001 caatcaggca tatagcgcgt gaattttacc cacgctatat acagtaaaaa ctattcgtgg
     9061 gacttcttga taaagaacgt gatagcagta cccaccacca cacaggtgac gacgatggtg
     9121 atcaggctca gaatacccac atcagacgac agcagttctt tcatcaatgc catgttaacc
     9181 tcccccggtc gtagtcgacc tgtatgccaa gtttgtttat ttgctgtgca cagtatgctt
     9241 cagtttaaaa attatatata ttgacccaca tcaaatatgt gctgggtgat ggcacagaca
     9301 gtccgccaag caggcgggcg gttttcagct cacacaaaat cagccacaat ccagactccc
     9361 tcaccactac atgccgcgtt cacgtggcca gaccgccatg agccttgatc tatcgcccac
     9421 ccctgcccaa gagcacgaag cccgcaagtg ggtcatcatc gtctatgtct tgcaaatcct
     9481 ctcgctgttc atcggcatta cgtcgatcat tggcatcatc atcaactaca tcaaactgga
     9541 cgatgtacgc ggcacctggc tggaatccca ctttcgctgg cagatgcgca ccttctggtt
     9601 ttccttgctg tgggccgtgc taggagggct gaccttctgg tttggcgtcg gcattgtcgt
     9661 gtggattgtg gccggcatct gggggctgta ccgcatcatc aagggcgcca tttatctata
     9721 cgacggcaaa accatgtata tgaacaccct gccctgagct gtgccgcccg catcggccgg
     9781 gcgctgtggc tttttggcga ccagcccgtc atgttgcagt gcaaggcgct tgcattgccc
     9841 ggcaaatcgc ggcacactgc cccgcataga agcgctcaca caatgacgcg aagcgtcccc
     9901 gacgccttgc cccgccgagc gaacaccagc ggggccatac agccaggggt ttttgtaggc
     9961 gcctctacaa agaagcgttt acaaaatcga atgtttgtct ttttgaagcc tgcatccatg
    10021 ccccaaaccg ccctgcgccc tctgctgtcc ctgtcgccct tgccggcagg cgtgctgctg
    10081 tccggcgtag tggtaagcgt agtggtccta gaccacacgc cgcgggctgc tggatagcgt
    10141 tcaggacgat ccgaatccaa ccaaaccccc gccggcgtga cgctcgcggg ggtttgtgct
    10201 tttcccgcct gcgtcgcccg gcctgcaata tggagaacaa ggcatgcatg cctcttccaa
    10261 cactgccgcg cacggcgaag ccgaatggtt gaccggcgct gaattaaccg ttcgtttgct
    10321 ggagcgccag ggtgtgcgcg tgctgtcggg cattcccggc ggcgccatcc tgccgtttta
    10381 cgacgccctg tcggcgtcca ccgaaatcca ccacgtgctg gcccgtcacg aacagggcgc
    10441 cggctttatc gcccagggca tggcgcgcac caccggccag gcggcggtgt gcctggcctc
    10501 gtccggcccg ggcgccacca atctgctgac cgcgattgcc gacgccaagc tggattccat
    10561 cccgctggtg gccatcaccg gccaggtgcc gcaaagcatg atcggcaccg acgcctttca
    10621 agaggtggac acctatggcc tgagcgtgcc gatcaccaag cataattttt tggtggacag
    10681 cgccgaagcg ctgctggaag tgattccgcg cgccttctcg ctggcgctgt ctggccgccc
    10741 cggcccggtg ctggtggaca tccccaaaga cgtgcaattg cagcgcatcg ccgtcagcca
    10801 ctggccggag ccgggttgcg cccaggccgc gccggcgccg gacgccgaca aaatcgccca
    10861 ggcggcgcag atgatcaatc aggccaagcg cccgattctg tatctgggcg gcggcgtggt
    10921 gcactctggc gcctccgaag ccgccgtgac cctggccgag caagccagcc tgccgaccac
    10981 catgacgctg atggcgctag gcgccatgcc ggtggaccat cccttgtcgc tgggtatgct
    11041 gggcatgcac ggcgcgccgt acaccaatct ggcgctggaa gaatgtgacc tgctgattgc
    11101 cgtgggcgcg cgctttgacg accgcgccac cggcaaggtg gcggcgttct gcccgaatgc
    11161 ggcgattatt cacatcgaca tcgacccgtc cgagctggac aagatcaaga ccgcccacgt
    11221 tggcattgct ggcgatgtcg gcgcagtgct ggcgcaactg ctgccgcagg tgcaggcttc
    11281 cctgcgcgaa gactggaacc agcgggtggc ccagctcaag gccgagcggc cgctgcaatt
    11341 gcccggcctg gacgacccgc gcagccatta cggcctggtg caggccgtgg ccgacgcgct
    11401 ggacgacagc gcctttgtcg ccaccgacgt cggccagcat cagatgtggg tggcgcaagc
    11461 ctacccgctg cgccgcccgc gccaatggct gacctctggc ggcctgggca ccatgggctt
    11521 tggcctgccc accgccatcg gcgcggcgct ggccaacccg gaacgcaccg tggtgtgctt
    11581 ctccggcgat ggcagcatct tgatgaatat tcaggaattc gccaccctgg ccgaagccgg
    11641 gctgaacgtg aaagtggtgc tgatgaacaa cgcctcgctg ggcctggtgc accagcagca
    11701 ggagctgttc tacggcgagc gcatttttgc ttcgcgcttt atcgcccagc cggacttcct
    11761 gcaaatcgcc cgtggttttc gcattgccga gctggacctg gacgccgccg ccgacccgcg
    11821 cgccgcgctc aagcaggcgc tgaatgcgcc cggcccgtgc ctgattcacg ccagcatcga
    11881 cgtgaaagaa aaagtctacc cgatggtgcc gccgggcgcc gccaacaaag aaatgatcgg
    11941 aggttaacca tgaccgcaga acaactcact cgcccggtgc tggaaatcga cgtgaataac
    12001 catcctggcg tgatgtcgca tgtggtgggg ctgttctccc gccgcgccta caatgtggaa
    12061 ggcatcatct gcgtgccggt gggcactggc gaaaccagcc gcatctggtt gcagatcaac
    12121 gaagacgagc gtctggagca gatcgtcaaa caggtggaaa agctggaaga cgtgctggtg
    12181 gtgcgccgtc atgacgccgg ccacccggca tttgccggcc tggaacgcta cgctggctga
    12241 ttgacgcaaa tctggcagta aaaaacacac gccccgcccg gttacccggc ggggcgtgtg
    12301 tttttacgtc aaagcaaaag cactgggggt cagaccgcag cttcagccgg agcagcttgc
    12361 ttggccttgg cttgcggcag atccaacagt tcggcgtttt cgtcgcagaa cttcaggccg
    12421 gcttcgtagc ccaccttgaa cagcagctcc agattgctgg ccgaccagtc catcacttgc
    12481 ggccaatgct ctttggggat gccgcccatc agatccacct tcagcagctt gcgcttttcc
    12541 ttgccggttt tgtggtcgat attgtgccga tactggaaca ggctgatatc gtccttggcg
    12601 atttccacca gcggggtgat gatcgaaccc acccaggcgt cgtacaggtc gcgcggcttg
    12661 cggatcagtt gatcagcgcc gaggatgtcg aaaatcacca gggtgtccag gtcgcagtgc
    12721 aggccttttt gcttggcgtc ttcgttgtcg ttgaccagcg ccttgaagtt cagcgtatca
    12781 atcgccgcgc cttcgatgta gtcctcgcca ttcagggtgt acggcgggta gatcagcggg
    12841 aaggaaaacg ccgcgcgcag atgggccggg gtgatgtcgc ccttgcccca gatggtcatc
    12901 tctgcgcgat tcatattgta ggcgttgata taaaactcgg gcttgagctt ggggatggcg
    12961 tcaaagtcga tcacctgctc ggcaaacggc aggtgggcgc acatgcccag gcttttgccg
    13021 ctcaggtccg acggcgacag ggtggccagg aacagcttgg ccatgtccga gaaaaagcca
    13081 tggcccatcg gcgaggacgg gtcgtacagc ggcttggtga acgggttgcg gttcatgatc
    13141 cagtcgcggt aggcggcggc ttgctcgccc ggcttcatga acaccttgaa gttcaccgga
    13201 aaacggttgt aaatggcgtc agacacgccc actttggccc agtcttccag cgcctggcgc
    13261 gggttgccgt ttttcggcgc agcatacaac aagcccatca gcgcgcctgc gccagaggcg
    13321 gaaatcacgt caaattctgc gcctttttcc agaaacgcca ccaaggcgcc ggccatcagc
    13381 gtggagttgg gcgcgccgcc gccaatcagc agggcttttt tgtgtttgtg ttgcggattc
    13441 accttggctg tcatgcctgt tttcttgtag aggggatgga gaaaacgccc cgccagccac
    13501 ggtgcgcccc agggtaaagg gccgttgttg gaagtggcgg ggatggcgcc gcaggggatt
    13561 cccgcacacg tgtgcgccag acgtgacaat cccggcaaag ccgggattgt gcggtgggtt
    13621 tgcttgccat gcgggtgcag agacacttta agtcatggca ggggcatttt caaatcaatg
    13681 cgccagctgg catgcgcaag tctgggagag tgtggtgatt ttgccgcacc gcagcatgat
    13741 gcacgcgggc aggcttgccg gtggccggca gcagacccaa gagcaggccg tgttcttcca
    13801 ggaattcttg gccggtctgg tagcccacct cgaacaggcg cttgaggttg ctttccgacc
    13861 agtcaaagat ttccggcagc caggcgtccg gcacattctt gtagaagtcc agttgcagca
    13921 cggtcttgcc atgctgggcc atctgcatct tgtacatctg ggtatcgctg tgggcgatct
    13981 tcaccagcgg ggtgatgatg gacttcaccc aagcatcgta cagattgcgc ggtttttgca
    14041 acaagcgata gctgcccagg atgtcggcga caatcaccac atcgacgtgg tcttccacct
    14101 cggggcgggt catcacttca aaattcagcg gctcgatcga agcgccttcg atataccact
    14161 ggccatccag ctcgtacggc gggtagatga acgggaaaga aaacgccgcg cggatgcgct
    14221 cggcagagag tttttccttg ccccagactt ccattctgga ctcgtccaga ttgaaggcat
    14281 tgaaataaaa ctcgccagcg tggtgatggg ccagatcaaa atcgacgtag tcttcgacaa
    14341 acggcaggtg agcgcacaaa cccaggcttt ttttgctgag gttgctcggg cacatcgacg
    14401 cccaggtcag ctgcatcatg tcgctgagca ggccgccttg cgggaacatg ctctggcacc
    14461 atttcatcca cggattgctc gccatcgatt gacggaattg atgagcatgg tcgcccggct
    14521 tcatgaatac cttgtggttg acgggaaaga actgataaag ctcgtcagac acgccaatct
    14581 gcgaccaacg ggtgagcgcc tcgatgcggt cgccaccacg cgggcagttg tacaacagac
    14641 ccatcagcgc gcctgcgccg gaagtcgaca tcacgtcaaa ctccatctcg cgctcggcaa
    14701 acgccaccag tgcgccagcg atcaggctgg aattaggggc gccgccgccc aggacaagtc
    14761 cggtcttcac gtggggggtg ctattcatca ttgttctcct gcgaactgaa ttcggtccag
    14821 ggcgcaaggt ggcaaataca gatccgggcc aaccttgcgt cattcttcca atgaaacagt
    14881 cagtctggtg atgcatgagg cagtgccgca tcgtcaggct ggcgtcagct agcacgccag
    14941 cctggctggt cacttgtcac aggtatgcgc tgacgggact ccccttttat tcctcgtcag
    15001 cacccgagac aaacgaatgc acacaacata tttcttgctg ttgcttttgg cctggttata
    15061 tgaccattgc cccagaaaaa agtaaatacg taaatatgct taccagctcc gataaattca
    15121 taaaaatgac tttatcatca ccggcgcacc gaaactgggc gcgccatttc atgctgcact
    15181 gcagcgtaaa gataagcagc aaatgggcca attgatctga ccacttgctg ataagccaga
    15241 aaattgctag ttcttaaaat tttttaccca atttcacaga attttgcttg ctgactgaac
    15301 gtttgttttg caaccgggcc ttgcatgcct gatggcaaaa actgccacac cggcagaaac
    15361 ctccagccat ggcggcagca gatgcaccct ttcagcacgc tctgctgcac gggcaacacc
    15421 ggcaagcccg ggcaagtggg cagaaaatca cgcagacaac gatggccaga acagtcctca
    15481 gcggcaaagc agaagacaga tgtgttgcgc gtcaactgtt gcgctgcaag atagaaccgc
    15541 cagcacatcc ccagcagatc accacacacg cgcacttgtc atgacgatga tgacagaacc
    15601 aaaaaaacac ctaggtatgc cgcacgggat gtcagcacac acagaactca ggtacactgc
    15661 acaccccggt tttttgattg ttatttaagg gaaaatctac aaatggaaca gtttgacaac
    15721 gtcagcgtaa tcaaaaaagc caacgtctat tttgacggtc gctgcgtcag ccacagcctg
    15781 attctggctg acggcgtacg caaatcggtg ggggtgatcc tgccgggcgc cacgctgacc
    15841 ttcaataccg gcgcaccaga aatcatggaa gtgctggccg gccagtgcag cgttcagctc
    15901 aaaggcgaaa gcacggcgca aaactatggc gcaggccagt cattctcggt gccaggcgac
    15961 agcgcgtttg acatcacggt gaccgacacc ctgcattacg tctgccactt cggctaagcg
    16021 ccacgcctgg ccagacacgc gacggcaaac agtacaagca gtacagcaag gtcggctcac
    16081 cacgtcagga gggtttcctc acctcacggg tggagccaac caatcaaaaa cgggcggatc
    16141 agccagtaca gttttctggc cgatcccgcc cgtgacattg actcagtgcg caaacaacgc
    16201 ctgatacgcc gcctcactga accccacctc cacccgaccg gcctgcacca gcaccgggcg
    16261 cttgatcagc gaaggctgtg ccagcagcaa ggcaatcgcg ccctcccggt gttcaacctg
    16321 tgcccggctg gcctcatcca gcttgcgcca agtagtgccc ttacgattga ccagcgcatc
    16381 cagcgtagcg ccttgcgcca gccagccctc aatgacttcg gcggtgatgc cgagcttttt
    16441 atagtcgtga aacgtcacct cgataccttg tgccgccagc caggcgcggg cttttttcat
    16501 ggtgtcgcaa ttgggaatac catacagaac gggggtggac atggggaaag ctccagggac
    16561 aaaaccggca gtgtagccgg aatgccgccg cagacaagcc acgggcccgg cagcatggct
    16621 ggccgggccc gcagcgcagg gtgcgcagcg cttatccctt gttggcggtc atgccgggct
    16681 tctcgtcgcg caaggcgcgg cgcaggattt tgccgacatt ggtcttgggc agttcgtcgc
    16741 ggaactcgac aaacttgggc accttgtagc cggtgaggtt ggccttgcag tgggcgagga
    16801 tgtctttctc ggtcagcgcc gggtccttgc gcaccacaaa caccttgacc acttcaccgg
    16861 acttctcgtc cgctacgccc acgcaggcca cctccagcac gcccggatga ctggccacca
    16921 cgtcttcgac ttcgttcggg tagacattaa agcccgacac cagaatcatg tcttttttgc
    16981 ggtccaccag tttgacaaag ccctccggcg tcatcatcgc catatcgccg gtggccagaa
    17041 agccctgctc gtcgatcacc ttggcggttt catccggacg ccgccaatag cccttcatca
    17101 cctgtgggcc gcgaatgcac agctcgccgg attcgccata cgccacctca tggccctggc
    17161 tgtcacgaat ctggatctcg gtagaaggga tgggcacgcc aatcgtacca ttgtattcgg
    17221 tcaggtccag cgggttcatg cacgccgccg ggctggtttc ggtcaggcca taggcttcaa
    17281 tcaggggcac gccagtcacg gccttccatt tatccgccac agctttttgc accgccatgc
    17341 cgccgcccag cgccagtcgc caggtggaga aattcaccga agcaaaatcc gggtgattga
    17401 gcagagcatt aaacagggtg ttcaccccgg tcatcgccgt caccgggtat tgcttgattt
    17461 ccttgataaa gcccgggatg tcgcgcgggt tggtgatcag gatgttcagc gcgccaattt
    17521 cggtgaacac catcaggttg gcggtcagcg aaaagatatg ataaagcggc agggcggtga
    17581 caatcacttc ctggccttcg cgcacgacag gcttcaccca ggcgccagcc tgtagcatat
    17641 tggcgacaat attgcggtgc agcagcatgg cgccctt
//
LOCUS       DAOPYX010000023        17614 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_3378_length_17614_cov_11.232644, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000023 DAOPYX010000000
VERSION     DAOPYX010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 17614)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17614)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17614
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3378_length_17614_cov_11.232644"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            96..1427
                     /locus_tag="PKN55_02305"
     CDS             96..1427
                     /locus_tag="PKN55_02305"
                     /inference="COORDINATES: protein motif:HMM:NF016462.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface lipoprotein assembly modifier"
                     /protein_id="HNX70108.1"
                     /translation="MCGSTSALAQEPAGLPALDRQVDEAFRQVLKTPASLDAGLKYAR
                     LLVAAGNYEGGVAALERLLLSPNPQPGIRVELAVLYYRLGSYEMAESLLRQALDDTRL
                     EGEPRTLAQTLLRDVSARNQPSQLRGMLMIGVRRQSNPSARSDADRVFAAGTLTPLAD
                     AYKPKSDTDIQLTARLDHRYDLGWQNEASVVSSLVAQITDYQSSSGRTLRANQENPYD
                     LAWGELTTGLRFKPAPIDLPGLRLRPYLILAELAAQGHRYLNNRGLGLEAEYQFNEKT
                     LASANYEYRHYHYSNRVDLPAAEQLGGPDHNLRLQLSRELAPGHLLSAELGLRQHQTG
                     RAYYDYQGEELRLTYSTSYPSPLTGVAGGYWSTAAWAGAAQRRYGAADPSITASQTRK
                     DTDWRIGLSQSVPLTGNWSMLLQVEHVRTHSNLPNYQARNTSLMGALAYRF"
     gene            1490..2329
                     /locus_tag="PKN55_02310"
     CDS             1490..2329
                     /locus_tag="PKN55_02310"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HNX70109.1"
                     /translation="MSPPPFYRDWWLAAALTTSTALPVGVAQAAQITQPVGVVTALPA
                     AAADKPDTLHVGSNITPGQRLQTGSDGPLHVLFLDQSALTLGPNSELTIEAFSYDADS
                     RQGQIRLGLSKGSLRVVGGYISKNNAAVITTPFATVEIQGGISLVSVTPNATQSTFLF
                     GQQMRVSNPGGNSTTVTRPGFSVNSGQDGPSAPTRMSPQQLTTQLSQLEQTRTPAGTP
                     STPSTPSPQLISTSDRPGQMGLPNQQLAPDRVAVNTDNTQQANPTLTLNNLLTTGAST
                     IQS"
     gene            2384..3415
                     /locus_tag="PKN55_02315"
     CDS             2384..3415
                     /locus_tag="PKN55_02315"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNX70110.1"
                     /translation="MRVLFVSNYPHLPDIHGGLQTTTHDLCLAIQAMGAAAGVLCGRQ
                     ASGPDAWQEDRTLGYPVWRATQPEHALAEVAARWQASAIVVQTGTSLLPMVVRSLATG
                     LPTAVYLHNVETHQLAGTLTPDPSLRYFANSDFTAERWRALCGIDCCVIPPIIDAERY
                     LVAQTGEQVLFVNPTPIKGVEILFALAAACPELPFLVYESWNLAPAWREHCQQRARAL
                     GNVRWQAPTLDMRQAYAQARVLLMPSIWEESFGRTVIEAQLNGLPVLASHRGALPALV
                     GQGGLTLEAHAPIADWAQALRHLYAQPEAAGAAGREQAMRHVAATPLRVGQLLGELAI
                     HAAAHRTDA"
     gene            3465..4259
                     /locus_tag="PKN55_02320"
     CDS             3465..4259
                     /locus_tag="PKN55_02320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX70111.1"
                     /translation="MPAHPLTDSLFAHYPRPQRQDCSFYHSFDLPEGEIIGQWDLRQH
                     ADQYLGGVALNQRSVLEVGPASGFLSFHMENQGAQVTCVEPPLSYLWDAVPFADYDLE
                     HWRQEFTAEIQKVRNSFWYVHHQKQSRVRLLETDPYAIPAAAGQFDVGLLASVLLHCR
                     RPFDLLESVARRTERTMIITEIWNPTLGEGPVCMLLPHQGMKQVHTWWHFTPQFFISA
                     LGILGFTEARVSLHTQRQPAENREVTLFTVVCERPESQATSTHRPR"
     gene            4262..9472
                     /locus_tag="PKN55_02325"
     CDS             4262..9472
                     /locus_tag="PKN55_02325"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNX70112.1"
                     /translation="MPETTLIDTLLTQARQQLALGDAEQALSLAQHAYACAPHRPEAT
                     LALGEILLHSGQYDAAHALLWPAAQQWPHVAALHRCLGLLEQQLGQREAAIDSLRWAC
                     TLDPSQHDAAFRLAWALHDQGELDEALYWACHALAGERNGPRLLQTGWLLQQTGQLAA
                     AAEHYQHAIAAYPAHAAEQGQLHLQLAECQLLLAHTAAAYATLDAGLRRFPDDPGLLT
                     TLAHGQWRGGDALAAIRTARRLTELDPECVAHWHLLGVFLQDSGDWLAADRCFAQVQQ
                     RDLSQSESLFRRAQIQARAGRPADARWLLQQVLHHRPDALPAYTLMAQVLLDLGEIDE
                     ARRLLLRQLRATPAHAEHWRLLALIQRQRGRPALARRALQRALRHDPSHTEAMRMLAW
                     LALEQHDAALALAMVHQLQARAPDDTHTHIQAAFVHAALGDLAQAGRHAERAVAQAPA
                     QAEAWRALSQVRYRQQRLREAEDAIDTALQLAPDRLDSLRQLGWILVAQQRLGHAELA
                     FLRAQEHAPNDPVVWLELAQVRLRAGQFAAGLACLDALQALGPLSPQAQLLQARLLVE
                     GGHSQPAYYTQALALCRRLLAQPSLQGEVAALLVRLRGLGVDAARPLCAMLPHSLWRL
                     SCQQAMNTAVTQHGHAYLARLAQLARQEFPEQPWLALACLYVDSLSAQASPAHLALMA
                     RHAWRALKLHNGLSTGWATRQPRQPGSRLRLAYVASQSHGRLLQRVLASHDPAQVEVF
                     VYSQQPLGDLPAHVHEQPLEPAELASACAANQIDILIDAGGLQPFEGQYALLERYAQR
                     LAPVQVAWLGSWGSAGGLFDVLLTDREAVPASAEQHYAEAIWTLDGGQWCWTPPAQAP
                     AVHAPPAASQGRVTFGVTARGLRLNDDTLRAYAGVLRGLPSARLRLIGAVSLDWPQRA
                     DLLARLHAHGVDASRVEFDPPSHYRDWLAWFQQVDIVLDSFPGNGGLSLLDALWMGVP
                     VVSRCGDWAGARQGASILRPLGLGHWVADSEAEFVATALALASDVPALCQIRTELRAR
                     MQASPLLDGQRVARQIEQHCAAYLARHAPAPTDGDLKQAVREHARRELATWLDKADTR
                     LSLPGVPAGDTPALSVVVILYNQAGLSLRTLQALADQRGVTFETLIIDNASSDQTDAL
                     LARVDGATIVRNADNLGFVLAANQGAALARGRHLLLLNNDAIVQQGALAATCRRLDAD
                     PTIGALGGRIVLMSGGLQEAGNVIFQDGSTAGIGRGEDPFSPAALCSRPADYCSGVYL
                     AVPLPLWRMLDGFDRDFVPAYYEDTDFCLRVWQAGFRVVYAPDIVVEHLEWGSAGNQE
                     AQQRMRDNRLRFLAKHGDWLSRQPRPGRVTLDHDRWRSPADTPRLPRVLIIDNEVPHM
                     ARGGGLPRARLMLQALQDWPVTLFPLWSFDDDWAQVHGSIPDTVEVMLGLGMAGLEAF
                     LERRRGLYDVLWISRPPNLQALAPLRARRPELFAGMRLIYDSEALFALREIGELAVKG
                     RPLTPAQAQARLDAELALAQDLSQVVVVSQRDAQPFRERGHPVSILSHAMPVRRSVPG
                     PKRRAGLVFVGALHPDTPNEDGLLWFVEQVLPRLRARPGGAPVLEVVGECRSPRIAAL
                     ASAEVRVLGAQADLRPCYDRARVFIAPVRFAGGVPVKVIEAAAQGIPVVASAILQRQL
                     GWQDGRDIQGARDAEAFARAIARLLDDDAHWQAQQAAAWARCEADYHPDDFARQVHRL
                     LLGEPTQEHA"
     gene            9469..10302
                     /locus_tag="PKN55_02330"
     CDS             9469..10302
                     /locus_tag="PKN55_02330"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNX70113.1"
                     /translation="MNPHELETAALRQSRRWLAQGQRREAEASLRQALARETNLPGVH
                     AALAQLRWPGPDYRAWLAWLHQHLQPQVYVEIGVEAGHSLALVQPGSRVLAIDPTPVG
                     DPLRHCPAPAQLFAQTSADFFAGAAQTQLAESGFNLAFIDGDHRFEQVLDDFIALEHY
                     AAPGAVVLLHDTLPLTESTAGGARHTGFYSGDAWKVLPCLRALRPDLRLTTLPTAPTG
                     LTVITGLDRHSRVLAERRSLIHRVYARLPSAHAVAHPQQLFNLGENNPAWLARWLANA
                     P"
     gene            complement(10244..11224)
                     /locus_tag="PKN55_02335"
     CDS             complement(10244..11224)
                     /locus_tag="PKN55_02335"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNX70114.1"
                     /translation="MSLVCPLGAIDHGLADQLDITWGDPDAWTAEGLHWTHLPQVRAA
                     INRKVSGAAEVSPLRWFFQAVAAEQPLPLNRVLVLGCGSGTLEREIAQAGWAREIVAI
                     DLSAKVLAVAQAQAQAEGLEGIRYFQADMNRLPVGQPPFTPGSFDAVLGVSSVHHCAN
                     LALLYRHIGLLLAPHGWFFLDEYVGPDQFQWPDSQIRQLNRLAELLPERLMTLRNGSC
                     KRHFRRPSVAEVVAVDPSEAICSSRLLPLLPDYFSQVQVRPYGGAILHMLLAGVAQNF
                     ADATAEPYLRSLMAAEDELYRAGQLPHDFACVMARSPASAPATPGCFRPD"
     gene            complement(11329..11955)
                     /gene="bioD"
                     /locus_tag="PKN55_02340"
     CDS             complement(11329..11955)
                     /gene="bioD"
                     /locus_tag="PKN55_02340"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012695659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HNX70115.1"
                     /translation="MSRAYFITGTDTEIGKTHSAVRLIERWRGEGLRVAAMKPVASGC
                     TLGADGELVNDDVERLIAASGQTDRDAMNPYRFAPPISPHLAARQAGVSIDLALLRQR
                     FAALQADADIVVVEGAGGWYAPLSDTLSMADLARALAIPVLLVVGVRLGCINHARLSA
                     EAIRASGLTLAGWIANQPVADVSCRDEVMATLTATLGAPPWLDIAYQQ"
     gene            complement(11952..12416)
                     /locus_tag="PKN55_02345"
     CDS             complement(11952..12416)
                     /locus_tag="PKN55_02345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019920510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1854 domain-containing protein"
                     /protein_id="HNX70116.1"
                     /translation="MHLDYTLTRNAFGQLELRRGDAVYPGVTAVRAFPIGAPEHGLSL
                     VSPDGHELAWIHHLSELPAEMRALIEAELAGREFMPTIRRICSVSSFATPSTWEVDTD
                     RGPTRFVLKGEDDIRRMAAPVLLISDSHGIHYLIRNPAELDKASRKLLDRFL"
     gene            complement(12432..14720)
                     /locus_tag="PKN55_02350"
     CDS             complement(12432..14720)
                     /locus_tag="PKN55_02350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012697437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNX70117.1"
                     /translation="MNANTLSLPAGPDLPDIWLPAVRGQLAAGENLLGWLELDLDAEL
                     RFAHGLIALTDRRLLSWGEAGDAARAWPLSAALELRHHDHAGVVNLELCDASQRLAQW
                     RYTLNVNAQALRLLAQWEAQLHLLRHGAPEDSDEDETPCCPSCHNPLPPDQDDCPVCA
                     RELHTPPSTWTLLRLWRFARPYRWQLLGGFLLTLASTAATLVPPYLTMPLMDNVLIPF
                     QNGKPIDWPLVSRYLGGLFAAAGLAWVLGWLRTYILALVSERIGRDLRTTTYEHLLKL
                     SQEYFGGKRTGDLMARIGSETDRINVFLSLHLLDFATDVLMIVMTSAILFSINPWLAI
                     VTLAPLPFIAWLIHYVRDKLRTGFEKVDRVWAEVTNVLADTIPGIRVVKAFAQEQREA
                     ERFREANRHNLAINDRLNKTWSLFSPTVTLLTEVGLLVVWAFGIWQISKGDITVGVLT
                     AFLAYISRFYTRLDSMSRIVSVTQKAASGAKRIFDILDHVSSVPEPAKPVHLPQISGR
                     IELRDVGFRYGNRAVSRGVSLDIQPGEMIGLVGHSGSGKSTLVNLICRFYDVSEGSIR
                     VDGVDIRSLPVAEYRRNIGLVLQEPFLFFGTIAENIAYGKPDASREEIIAAARAAHAH
                     EFILRLPHGYDSLVGERGQALSGGERQRISIARALLINPRILILDEATSSVDSTTEKE
                     IQKALDNLVKGRTTIAIAHRLSTLRKADRLVVLDRGRVVEEGSHDALMARQGAYWQLY
                     QAQARQAEADRAEAAAEWGVAP"
     gene            complement(15069..15899)
                     /locus_tag="PKN55_02355"
     CDS             complement(15069..15899)
                     /locus_tag="PKN55_02355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX70118.1"
                     /translation="MAEWNRNTPWRQGHLLSQDAITTLGLRHAARSEQTVVIVASHDC
                     DLAQAPEGEPKIEIVIGCLVTDKDGNCTHAKNARKLHVEFVGGDTFWAEFEATAKSSI
                     EKLKLNEFYPRSDVHLTAENHTIFQMWLASRYRRSAFPDEFERRLTRETKLADKIAKA
                     VRPHGELITGVFFDVDEGAEVVRSGPDDTYTLDISILYDPDVDAAEHAADEVANTIKT
                     AFKDKLFTSTQQWLHIELRFCEHIAESTLSYADFKRLKRWRLEHISLAADPQQSVLAE
                     "
     gene            complement(15909..16535)
                     /locus_tag="PKN55_02360"
     CDS             complement(15909..16535)
                     /locus_tag="PKN55_02360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX70119.1"
                     /translation="MDTAMTYGGWVTDRPAATGVSAAVVRFVGWMRGVLVAGLTRVST
                     GGGQSIERLQRSTQLLPHIASTSKVVDRTPAEDLARIREVLKPAVSDLATTFDVSRQS
                     VYNWLNGEPVADKNVAKLKDLAHAADVLAYEGVVVNAALLKRKFANGRTLLQVAQAGE
                     SAREAANQLVRIYKREAAQRERIAAQFANRTKTPSSADFDLPSANDLA"
     gene            complement(16547..17344)
                     /locus_tag="PKN55_02365"
     CDS             complement(16547..17344)
                     /locus_tag="PKN55_02365"
                     /inference="COORDINATES: protein motif:HMM:TIGR04255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04255 family protein"
                     /protein_id="HNX70120.1"
                     /translation="MGTKLSSAPLYYTVAQVQFNPVLDLDGYIPAIQSKMRETHFSDY
                     KKEVVQRLVFPFGNAEQDQMAAPTVASQSRYLFGDIDGRSSFMLETNTLSFQTTRYDT
                     FETFSETLLGGLRILHNALGLDFVERIGLRYLNAIQPSKEESLRAFLVPEVLGHALLR
                     GEGGKLQHSVSETSFLTAAGQLVSRVLIRDGFIGLPMELSDLAPTIDPRFTQCESLHA
                     IVDTDAAIVRREAFQLEKIDTYLTALHDEIVKSFEATVTDHARVSWA"
BASE COUNT         3171 a         5991 c         5546 g         2906 t
ORIGIN      
        1 catacgttgc actcactgca aggagctgcc aattggccca tctgcccaag cgactgcgtt
       61 cccgtccagg acactgggcc ctcggcgctg ccctgttgtg tggcagcacc agcgccctgg
      121 cccaagagcc cgccggcctg ccggcgctgg atcgtcaggt agacgaagct tttcgccagg
      181 tgctgaaaac cccggccagc ctggatgccg ggctgaaata cgcccgcctg ctggtggccg
      241 ccggcaacta cgaaggtggc gtggccgcgc tggagcgctt gctgctgagc cccaacccgc
      301 aaccgggtat ccgcgtggaa ctggcggtgc tgtactaccg gttggggtcg tatgaaatgg
      361 cagaaagcct gttgcgtcag gcactggacg acacccggct ggaaggcgag ccgcgcacgc
      421 tggcgcaaac cctgttgcgc gatgtcagtg cacgcaacca gccctcgcaa ctgcgcggca
      481 tgctgatgat cggcgtacgc cgacaaagca accccagcgc ccgcagcgac gccgaccggg
      541 tatttgccgc tggcacgctc accccgctgg ccgacgccta caagcccaaa tccgacactg
      601 acatccagct gaccgcccgg ctggatcacc gctacgactt gggctggcag aacgaagcgt
      661 ccgtggtcag ctcactggtg gcgcagatta ccgattatca gtcgtccagc ggccgcacct
      721 tgcgcgccaa ccaggaaaac ccctacgacc tggcctgggg cgagctgacc accggcctgc
      781 gcttcaaacc cgcccccatc gacctgcccg gcctgcgcct gcgcccgtat ctgattctgg
      841 ccgagctggc cgcgcagggg catcgctatc tgaacaaccg tggcctgggc ctggaagcgg
      901 aataccagtt caacgaaaaa accctggcca gcgccaacta cgaataccgc cattaccact
      961 acagcaatcg ggtagatctg cctgccgccg agcaactggg tggcccggac cataatctgc
     1021 gcctgcaact gagccgggag ctggcccccg gccacctgct gagcgccgag ctgggcctgc
     1081 gccagcacca gaccggccgc gcctactatg attaccaagg tgaagaactg cggctgacct
     1141 actccaccag ctatcccagc ccgctgaccg gcgtggccgg tggctactgg agcaccgccg
     1201 cgtgggcggg cgccgcccag cgccgctacg gcgcagcaga ccccagcatc accgccagcc
     1261 agacgcgcaa ggacaccgac tggcgaattg gcctcagcca atcggtgcca ctgaccggca
     1321 actggtcgat gctgctgcaa gtggaacatg tccgcacgca ctccaacctg cccaattacc
     1381 aagccagaaa cacctcgctg atgggtgcgc tggcctaccg tttttgatgg atacgcacca
     1441 tgcatcctga cctgacccaa cctggcgccg atcgcccaag gagcctgcca tgagcccacc
     1501 acccttttac cgtgactggt ggctcgccgc cgccttgacc accagcacag ccttgccggt
     1561 gggcgtggcc caggccgcac aaatcaccca gccggtaggc gtggtgacgg cgctgccggc
     1621 cgccgccgcc gacaaacccg acaccttgca tgtcggcagc aacatcaccc ccggccagcg
     1681 cctgcaaacc ggcagcgatg gcccgctgca tgtgctgttt ctggatcagt ccgcgctgac
     1741 gctcggcccg aactccgagc tgaccatcga agcattcagc tacgatgccg acagccggca
     1801 ggggcagatt cgcctggggc tgagcaaggg ctccttgcgc gtggtggggg gctacatcag
     1861 caaaaacaac gcagcagtca tcaccacgcc ctttgccacg gtagaaatcc agggcggcat
     1921 cagcctggtg tccgtcacgc caaacgccac ccaatccacc tttctgtttg gccagcaaat
     1981 gcgggtcagc aatccgggcg gcaacagcac aaccgtcacc cggccgggct tttcggtgaa
     2041 cagcggtcaa gacggcccat ccgcgcccac ccggatgtcg ccgcagcaac tgaccaccca
     2101 gctctctcag ctggagcaaa cccgcacacc agccggcacg ccctccacgc cgagcacgcc
     2161 gtcgccgcag ctgatcagca ccagcgaccg gcccggccag atgggcctgc ccaaccagca
     2221 gttggcgccc gaccgggtgg cggtcaatac cgacaatacc cagcaagcca accccacgct
     2281 gacgctgaac aacctgctga ccaccggcgc cagcacgatt caatcctgac cgcaggcggg
     2341 gcgcacgccc cgctcgtggc gtttttccgc caggcaggcc tgcatgcgcg tgctttttgt
     2401 ctctaactat ccccacttgc cggatatcca cggcggtctg caaaccacca cccatgacct
     2461 gtgcctggcc atccaggcca tgggcgcagc ggcgggcgtg ctgtgtggcc ggcaagccag
     2521 tgggccagat gcctggcaag aagaccgcac actgggctac ccggtatggc gcgccacgca
     2581 gccagagcac gcactggccg aggtggccgc ccgctggcag gccagcgcca tcgtggtaca
     2641 aaccggcacc agcctgctgc cgatggtggt gcgcagcctg gccaccggcc tgcccaccgc
     2701 ggtgtacctg cacaatgtgg aaacccacca gctggccggc acgctgacgc cagatccctc
     2761 gctgcggtat tttgccaatt ctgatttcac cgccgagcgc tggcgcgcgt tgtgcggcat
     2821 cgactgctgc gtgattccgc cgattatcga cgccgagcgc tacctggtgg cacagacggg
     2881 cgagcaggtg ttatttgtca atcccacgcc gatcaagggc gtggaaatcc tgtttgccct
     2941 ggccgctgcc tgcccggaac tgccgttttt ggtgtacgaa agctggaacc tcgccccagc
     3001 ctggcgcgag cactgccagc agcgcgcccg cgcgctgggc aatgtccgct ggcaagcgcc
     3061 aacgctggac atgcgccagg cctacgccca ggcccgtgtg ctgctgatgc cgtcgatctg
     3121 ggaagaatcc ttcggccgca ccgtcatcga agcccagctc aacggcctgc cggtgctggc
     3181 cagccaccgg ggtgctttgc cggcgctggt gggccagggt ggcctcaccc tggaagccca
     3241 cgcgccgatt gccgactggg cgcaagcctt gcgccacctg tatgcccaac cagaagcagc
     3301 cggcgccgcc ggccgcgaac aggccatgcg ccatgtggcc gccaccccgc tgagggtggg
     3361 ccagttgctg ggcgagctgg ccatccacgc cgctgcccac cgcactgacg cctgatttca
     3421 ttcattgccc acggctggct cgccagccac aaggacttca acacatgccg gcccacccgc
     3481 tcaccgactc gctgtttgcc cactaccccc gcccccagcg gcaggattgc agtttttacc
     3541 actcgttcga cctgcccgaa ggcgagatca tcggccaatg ggatttgcgc caacatgccg
     3601 atcaatacct gggcggcgtg gcgctgaacc agcgctcggt gctggaagtc ggcccggcca
     3661 gcgggttttt gtcgtttcac atggaaaacc agggcgccca ggtgacttgc gtagagccgc
     3721 cgctgtccta tctgtgggac gccgtgccgt ttgccgacta cgatctggag cactggcggc
     3781 aagagttcac cgccgaaatc cagaaagtgc gcaactcgtt ctggtatgtc catcaccaga
     3841 aacaatcgcg cgtgcgcctg ctggaaaccg acccctacgc cattccggcc gccgccggcc
     3901 agtttgacgt cggcctgctg gcctcggtgc tgctgcactg ccgccggcca ttcgacctgc
     3961 tggaaagcgt ggcgcggcgc accgagcgca cgatgatcat caccgaaatc tggaacccca
     4021 ccctcggcga ggggccggtg tgcatgctgc tgccgcatca gggcatgaaa caggtgcaca
     4081 cctggtggca tttcaccccg cagttcttta tctcggcgct gggcattctg ggctttaccg
     4141 aagcgcgcgt cagcctgcac acccagcgtc aacctgccga aaaccgcgaa gtcaccctgt
     4201 tcaccgtggt gtgcgaacgc cccgagtcgc aggccacttc gacccaccgt ccgcgctgaa
     4261 catgcccgaa accaccctga tcgacacctt gctgacccag gccaggcaac agctggcgct
     4321 gggcgacgcc gaacaggcgc tcagcctggc ccagcacgcc tacgcctgcg ccccgcaccg
     4381 cccggaagcc accctggcgc tgggcgaaat cctgctgcac agcggccagt acgacgccgc
     4441 ccatgccctg ctgtggcccg ccgcccagca atggccgcat gtggccgccc tgcaccgctg
     4501 ccttggcctg ctggaacagc agttgggcca gcgcgaagcc gccatcgaca gcctgcgctg
     4561 ggcctgcacg ctggacccca gccagcacga cgctgccttc cggctggcct gggcactgca
     4621 cgaccaggga gagctggacg aagccctgta ctgggcctgc catgcgctgg ccggcgaacg
     4681 caacggcccg cgcctgctgc aaaccggctg gctgctgcaa caaaccggcc agcttgccgc
     4741 tgccgccgag cactatcagc acgccatcgc cgcctacccg gcccacgccg ccgaacaagg
     4801 ccaactgcac ctgcaactgg ccgaatgcca actgctgttg gcacataccg ccgccgcata
     4861 cgccaccctg gacgccgggc tgcgccgttt tcctgatgac cccggcctgc tgaccaccct
     4921 ggcgcatggg caatggcgcg gcggcgacgc gctggcggcc atccgcacag cccgacggct
     4981 gaccgaactc gaccccgaat gcgtggccca ttggcatctg ctgggggtgt ttctgcagga
     5041 ttccggcgac tggttggccg ccgatcgctg ctttgcccaa gtgcagcaac gcgacctgag
     5101 ccaaagcgaa tcgctgtttc gccgcgcgca aatccaggca cgcgccggtc gcccggccga
     5161 cgcccgctgg ctgctgcaac aggtgctgca ccaccgcccc gacgccctcc ccgcctacac
     5221 cctgatggcg caggtgttgc tggatctggg cgagatcgac gaggcccgcc ggctgctgct
     5281 gcgccagctg cgcgccacgc ccgcgcatgc ggaacactgg cggctgctgg cgctcatcca
     5341 gcgccaacgt ggccggccgg cactggcgcg gcgcgcacta cagcgcgccc tgcggcacga
     5401 cccgagccac accgaagcca tgcgcatgct ggcctggctg gcgctggaac aacacgatgc
     5461 cgcgctggcg ctggccatgg tgcatcagct gcaggcccgc gcgcctgacg acacccacac
     5521 ccatatccag gcagcttttg tgcacgccgc gctgggcgac ctggcccagg ccggccgcca
     5581 tgccgagcgg gcagtggcgc aagcgcccgc gcaggccgaa gcctggcgcg cgctgtcgca
     5641 ggtgcgctac cggcaacagc gcctgcgcga agccgaagac gccatcgaca ccgccctgca
     5701 actggcgccc gaccggctgg acagcctgcg ccagctgggc tggattctgg tggcgcagca
     5761 gcgcttgggg cacgccgaac tggctttttt gcgcgcgcag gaacacgccc cgaatgatcc
     5821 ggtggtctgg ctggagctgg cgcaggtgcg gctgcgcgcc ggccagtttg ccgccggcct
     5881 ggcctgcctg gacgccttgc aggcgctggg gccgctgtcg ccacaggcgc agctgctgca
     5941 agcgcgcctg ctggtggaag gcggtcacag ccaacccgcc tactacaccc aggccctggc
     6001 gctgtgccgc cgcctgctgg cgcaacccag cctgcagggc gaagtggcag cactactggt
     6061 gcgtttgcgc gggctggggg tagacgccgc gcggccgctg tgcgccatgc tgccacactc
     6121 gctatggcgg ctcagttgcc agcaggccat gaacaccgcc gtcacccaac acggccacgc
     6181 ctatctggcc cgactggccc aattagcccg gcaggagttt cccgaacaac cctggctggc
     6241 cctggcctgc ctgtatgtag acagcctgtc ggcccaggcc agcccggcgc atctggcgct
     6301 gatggcccgc cacgcctggc gggcgctgaa actgcacaac ggcctgtcca ccggctgggc
     6361 cacacgccag ccccgccagc cgggcagccg gctgcggctg gcttatgtgg ccagccagtc
     6421 gcacggccgt ttgttgcagc gggtgctggc cagccacgac cccgcacaag tcgaagtgtt
     6481 tgtgtacagc cagcagccgc tgggtgattt gccggcgcat gtgcacgagc agccactgga
     6541 gccggccgaa ctggccagtg cctgcgccgc caaccagatc gacatcctga tcgacgccgg
     6601 cggcctgcaa ccgtttgaag ggcaatacgc cctgctggag cgctacgcgc aacggctggc
     6661 gccagtgcaa gtggcctggc tgggcagctg gggcagcgcc ggcgggctgt ttgacgtgct
     6721 gctgaccgac cgcgaagccg tgccggccag cgccgaacag cactacgccg aagccatctg
     6781 gacgctggac ggcggccaat ggtgctggac gccaccggcc caggcgccgg ccgtgcacgc
     6841 cccgccggcc gccagccagg gtcgggtgac gtttggcgtc accgcccgtg gcctgcgcct
     6901 gaacgacgac accctgcgcg cctatgccgg cgtgctgcgc ggtctgccca gcgcgcgcct
     6961 gcgcctgatt ggcgcagtca gcctggattg gccacaacgc gccgacctgc tcgcccgact
     7021 gcacgcgcac ggcgtggacg ccagccgggt ggaattcgac ccacccagcc actaccgcga
     7081 ctggctggcc tggttccagc aggtggacat cgtgctggac agttttccgg gcaatggcgg
     7141 gctgagcctg ctggatgcgc tatggatggg cgtgccggtg gtcagccgct gcggcgactg
     7201 ggccggcgcc cgccaggggg cgtccatcct gcgcccgctg gggctggggc actgggtagc
     7261 ggacagcgag gcggagtttg ttgccaccgc gctggccctg gccagcgatg tgccggcctt
     7321 gtgccagatt cgcaccgaac tgcgcgcccg catgcaggcc tcgcccttgc tggatggcca
     7381 gcgggtggcc cggcaaatcg aacaacactg cgccgcctat ctggcccgcc atgccccggc
     7441 gcccactgac ggcgacctca aacaagcggt gcgcgagcac gcccgccgcg agctggccac
     7501 ctggctggac aaagccgaca cccggctgtc gctgcccggc gtgccggcgg gagacacccc
     7561 ggcgctgtcg gtggtggtga ttttgtataa ccaggccggg ctgagcctgc gcaccctgca
     7621 agccctggcc gaccagcgcg gggtgacctt cgaaacgctg atcatcgaca acgcctccag
     7681 cgaccagacc gacgccctgc tggcgcgggt ggacggcgcc accatcgtgc gcaatgccga
     7741 caacctgggc tttgtgctgg cagccaatca gggcgcggcc ctggcacgcg gccgccatct
     7801 attgctgctg aacaacgacg ccatcgtcca gcagggcgcg ctggccgcca cctgccggcg
     7861 gctggacgcc gaccccacca ttggcgcgct gggcgggcgc attgtgctga tgtctggcgg
     7921 cctgcaagaa gccggcaatg tgatttttca ggatggctcc accgccggca ttggccgggg
     7981 tgaagacccc ttctcgccag cggcactgtg cagccgcccg gctgattatt gctcgggcgt
     8041 gtatctggcc gtgccgctgc cgctgtggcg catgctggac ggctttgacc gcgattttgt
     8101 gccggcttat tacgaagaca ccgatttttg cctgcgcgtc tggcaggccg gttttcgggt
     8161 ggtgtacgcg ccggacatcg tggtggaaca cctggaatgg ggcagtgccg gcaatcagga
     8221 agcccagcag cgcatgcgcg acaaccgcct gcgctttctg gccaaacatg gcgactggct
     8281 cagccgccag ccgcgtccgg gccgggtgac gctggatcac gaccgctggc gcagcccggc
     8341 cgacacccca cgcctgccgc gcgtgctgat catcgacaat gaagtgccgc acatggcgcg
     8401 cggcggtggc ctgccgcgcg cgcggctgat gctgcaagcc ctgcaagact ggccggtgac
     8461 gctgtttccg ctgtggtcat tcgacgacga ctgggcacag gtgcatggct cgattcccga
     8521 cacggtcgag gtgatgctgg gcctgggcat ggccgggctg gaagcgtttc tggaacgtcg
     8581 gcgcggcctg tacgacgtgc tgtggatcag ccgcccgccc aatttgcagg cgctggcgcc
     8641 gctgcgcgcc cgtcggccag agttgtttgc cggcatgcgg ctgatttatg actcggaagc
     8701 gctgtttgcc ctgcgtgaaa tcggcgaact ggcggtcaag ggccggccgc tgacgccggc
     8761 acaggcgcag gcgcggctgg acgccgagct ggcgctggcg caagacctca gccaggtggt
     8821 ggtggtgtcc cagcgcgacg cccagccttt ccgcgagcgc ggccacccgg tgagcattct
     8881 cagccacgcc atgccagtgc ggcgcagcgt gcccggcccg aagcggcgcg ccggcctggt
     8941 gtttgtcggc gccctgcacc cggacacccc caacgaagac ggcctgttgt ggtttgtcga
     9001 gcaggtgctg ccccggctgc gcgcccgacc gggcggcgcg ccggtgctgg aggtggtggg
     9061 cgaatgtcgc tcgccacgca ttgctgccct ggccagcgcc gaggtgcgcg tgctgggcgc
     9121 ccaagccgac ctgcgcccct gctacgaccg cgcccgcgtg ttcatcgccc cggtgcgctt
     9181 tgccgggggc gtgccggtca aagtgatcga agccgccgcc cagggtattc cggtggtggc
     9241 ctcggccatc ctgcaacgcc agttgggctg gcaggatggc cgggacatcc agggcgcacg
     9301 cgatgccgag gcctttgccc gcgccattgc ccgcctgctg gacgacgacg cccactggca
     9361 ggcacaacag gccgccgcct gggcgcgctg cgaagccgat tatcaccccg acgactttgc
     9421 ccgccaggtg caccgcctgc tgctgggcga gccgacgcag gagcacgcat gaatccgcat
     9481 gagctggaaa ccgccgccct ccgccagtct cgccgctggc tggcccaagg ccagcgccgt
     9541 gaggccgaag ccagcctgcg tcaggcgctg gcgcgggaga ccaatctgcc cggcgtgcat
     9601 gccgcgctgg cgcaactgcg ctggccgggg ccggactatc gcgcctggct ggcctggctg
     9661 catcagcatc tgcaaccgca ggtgtatgtg gaaatcggcg tcgaagccgg ccattcgctg
     9721 gcgctggtgc agccgggcag ccgcgtgctg gccatcgacc cgaccccggt gggcgatcca
     9781 ctgcgccatt gcccggcgcc agcgcagtta ttcgcccaga ccagcgccga tttttttgcg
     9841 ggcgcggcgc agactcagct ggcagaaagt gggtttaacc tggcgtttat tgatggcgac
     9901 catcgctttg aacaggtgct ggatgatttc atcgcgctgg aacattacgc cgcgccaggg
     9961 gcggtggtgc tgttgcacga caccctgccg ctgaccgaat ccaccgcagg cggcgcccgg
    10021 cacaccggct tttacagcgg cgacgcctgg aaagtgctgc cctgcctgcg cgccctgcgc
    10081 ccggatttac ggctgaccac cctgcccacc gcgcccaccg ggctgacggt gatcaccggg
    10141 ctggaccggc attcccgcgt gctggccgag cggcgcagcc tgattcaccg ggtgtacgcc
    10201 cgcctgccca gcgcccacgc cgtggcgcac ccgcagcagc tgttcaatct gggcgaaaac
    10261 aacccggcgt ggctggcgcg ctggctggcg aacgcgccat gacgcaggca aagtcgtggg
    10321 gcagctggcc ggcgcgatac agctcgtcct cagcggccat caggctgcgc aaataaggtt
    10381 cggccgtggc atcggcaaaa ttctgcgcca cgccggccag cagcatgtgc aggatggcgc
    10441 cgccgtatgg gcgcacttgc acctggctaa agtagtctgg cagcagcggc aacaggcggg
    10501 atgagcaaat cgcctcgctg gggtccaccg ccaccacttc ggccacgctg ggccgacgaa
    10561 aatggcgctt gcaactgcca ttgcgcagcg tcatcagccg ctcgggcagc agctcggcca
    10621 ggcggttaag ctggcgaatc tggctgtctg gccattgaaa ctgatccggg ccgacgtatt
    10681 catccagaaa aaaccagcca tggggcgcca gcagcaggcc gatatggcgg tagagcaggg
    10741 ccagattggc gcagtggtgc acgctggaca cccccagcac ggcgtcaaaa ctgccggggg
    10801 taaacggcgg ctgccccacc ggcaggcggt tcatgtcggc ctgaaaatag cggatgccct
    10861 ccagcccctc ggcctgcgcc tgagcctgcg ccaccgccag caccttggcc gacaaatcaa
    10921 tcgccacgat ctcgcgcgcc cagccagcct gggcgatttc ccgctccagc gtgccgctgc
    10981 cgcatcccag caccagcacc cggttcaagg gcagcggctg ctcggcggcc accgcctgaa
    11041 aaaaccagcg cagcggcgac acctccgccg caccgctgac cttgcggtta atcgccgcgc
    11101 gcacctgcgg cagatgggtc cagtgcaagc cttcggcagt ccaggcatcc gggtcgcccc
    11161 aggtgatgtc cagttggtcg gccagaccgt gatcaatggc gcccagcggg cagacaagtg
    11221 acatgggaca aatcgcaaaa aattcagaga ggcccagtct agcatgggca gacagcggcg
    11281 ctcgcaaccc ccgtctggcg tgggccaggc aggggttgtg ggccgcgctt actgctggta
    11341 ggcgatatcc agccagggcg gcgcgcccag ggtggcggtc agcgtggcca tcacctcatc
    11401 gcgacagctg acgtcggcca ccggctggtt ggcaatccag ccggccaggg tcagcccgct
    11461 ggcgcgaatc gcctcggcgc tcagccgggc gtggttgatg cagcccagcc gcacgcccac
    11521 caccagcagc accggaatcg ccagcgcgcg cgccaggtcg gccatgctca gggtgtcgga
    11581 cagcggcgca taccagccgc cagcgccttc caccaccacg atgtcggcgt cagcctgcaa
    11641 ggcggcaaag cgctggcgca gcagcgccag atcaatgctc acgccagcct ggcgggcggc
    11701 caggtgcggg gaaatcggcg gggcaaagcg gtaggggttc atcgcgtcgc ggtcggtttg
    11761 cccgctggcg gcaatcagcc gctcgacgtc gtcgttgacc agctcgccat cggcgcccag
    11821 cgtgcagccg gacgccaccg gcttcatcgc cgccacgcgc aggccttcgc cgcgccagcg
    11881 ctcgatcaag cgcaccgcgc tgtgggtttt gccgatttcg gtgtcggtgc cggtgatgaa
    11941 ataggcgcgg ctcacagaaa gcggtccagc agcttgcggc tggctttgtc cagctcggcg
    12001 gggttgcgaa tcaggtagtg aatgccgtgg ctgtcgctga tcagcagcac gggcgcggcc
    12061 atgcggcgga tgtcgtcctc gcccttgagc acaaagcggg tggggccccg gtcggtgtcc
    12121 acttcccagg tgctgggggt ggcaaagctg ctgaccgagc aaatgcggcg aatggtgggc
    12181 atgaactcgc gcccggccag ctcggcttcg atcagggcgc gcatttcggc cggcagctcg
    12241 ctgaggtgat gaatccacgc cagttcgtgg ccatccgggc tgaccagcga caagccatgc
    12301 tcgggcgcgc caatcgggaa ggcgcgcacg gcggtgacgc cggggtaaac cgcgtcgccc
    12361 cggcgcagct ccagctggcc aaaggcgttg cgggtaaggg tgtaatcaag gtgcattgct
    12421 ttctccgcca atcagggcgc cacgccccat tcggccgccg cttcggcgcg gtcggcttcg
    12481 gcctggcgcg cctgcgcctg gtacagctgc cagtaagcgc cctggcgcgc catcagcgcg
    12541 tcgtggctgc cttcttccac cacgcggcca cgatccagca ccaccagccg gtcggccttg
    12601 cgcagggtgg acaggcggtg ggcaatggca atcgtggtgc ggcccttgac cagattatcc
    12661 agcgccttct ggatttcttt ttcggtggtg gaatccaccg aagaagtcgc ctcgtccaga
    12721 atcaaaatgc gcgggttgat cagcagcgcg cgggcaatcg aaatgcgctg gcgctcgccg
    12781 ccagacagcg cctggccgcg ctcgcccacc agcgagtcat agccatgcgg caggcgcagg
    12841 atgaattcgt gcgcgtgcgc ggcgcgggcg gcggcgatga tttcttcgcg gctggcgtcc
    12901 ggcttgccgt aggcgatgtt ctcggcaatg gtgccaaaaa acaggaaagg ctcttgcagc
    12961 accaggccga tattgcggcg gtattccgcc accggcagcg agcggatgtc cacgccatcg
    13021 acgcgaatcg acccttccga cacatcgtaa aaccggcaga tcaggttcac cagcgtgctc
    13081 ttgcccgagc cggaatgccc gaccaggcca atcatctcgc cgggctgaat atccaggctg
    13141 acgccccggc tgaccgcgcg gttgccatag cgaaagccca catcgcgcag ctcgatgcgc
    13201 ccggaaatct gcggcagatg caccggcttg gccggctcgg gcacgctgga aacatggtcg
    13261 agaatgtcaa aaatccgctt ggcgcccgac gccgcttttt gcgtgaccga gacaatccgg
    13321 ctcatcgaat ccagccgggt gtaaaagcgg ctgatatagg ccaggaaggc ggtcagcacg
    13381 ccgacggtga tgtcgccctt ggaaatctgc cagatgccaa acgcccacac caccagcagg
    13441 ccgacttcgg tcagcagggt gacggtgggc gaaaacaaag accaggtttt gttcagccgg
    13501 tcgttgatgg ccagattatg gcggttggcc tcgcgaaagc gctcggcctc gcgctgctcc
    13561 tgggcaaagg ctttgaccac gcgaatgcca gggatggtgt cggccagcac attggtgact
    13621 tccgcccaca cccggtccac tttttcaaag ccagtgcgca gcttgtcgcg cacatagtga
    13681 atcagccagg cgataaacgg cagcggcgcc agggtgacaa tcgccagcca ggggttgatg
    13741 gaaaacaaaa tcgccgaggt catgacgatc atcagcacat cggtggcgaa atccagcaga
    13801 tgcagcgaca aaaacacatt gatgcggtcg gtttccgagc caatccgcgc catcaggtcg
    13861 ccggtgcgct tgccgccaaa atactcctgc gacaacttca gcaagtgctc ataggtggtg
    13921 gtgcgcaaat cgcggccaat gcgctcggac accagcgcca gaatataggt gcgcagccag
    13981 cccagcaccc aggccagccc ggcggcggca aacagcccgc ccagatagcg gctgaccagc
    14041 ggccagtcga tgggcttgcc gttctggaac ggaatcagca cattgtccat cagcggcatg
    14101 gtcaggtagg gcggcaccag ggtggcggcg gtggacgcca gcgtcagcaa aaacccgccc
    14161 agcaactgcc agcggtaggg ccgggcaaag cgccacagcc gcagcaaggt ccaggtggac
    14221 ggcggcgtgt gcagctcgcg ggcgcacacc gggcagtcgt cctgatcggg cggcagcggg
    14281 ttgtggcagc tggggcagca tggggtttcg tcttcgtcgc tgtcttccgg cgcgccgtgg
    14341 cgcagcagat gcagctgcgc ctcccactgc gccagcagcc gcagcgcctg ggcgttgacg
    14401 ttcagcgtgt agcgccattg cgccagccgc tggctggcgt cgcacagctc cagattgacc
    14461 acgccggcat ggtcgtggtg gcgcagctcc agcgcggcgc tcagcggcca ggcgcgcgcc
    14521 gcgtcgccgg cctcgcccca gctcagcagg cggcggtcgg tcagcgcaat caaaccatgg
    14581 gcaaaacgca gctcggcgtc gagatccagc tccagccagc ccagcaggtt ttcaccggcg
    14641 gcaagctggc cgcgcacggc cggcagccag atgtctggca gatccgggcc ggcgggcagg
    14701 gaaagggtgt tggcgttcat gttcgggcgg catccgcaaa ttcagccggc ttctggcgca
    14761 acgcccaaaa gccgtggccc cggacgaagc gcccggggcc gacagggcat cggcgcgtgg
    14821 gcgcgatgca gggttgccgg gaagtataac tgaaatcggt gccagggctg gcttggcagc
    14881 ggtagggcgg gccgcttgca tgaattcaaa tgggacgaaa ctccaagaat tgctaccttt
    14941 tcgcctcaga aaggctgtga ggctacacgc agcgggcaag agcgaccctg acttaaattg
    15001 agcgttttga gacgtaaccc aagtgtacag ttgtcgctca aggggtttct ctatcagatt
    15061 cgaggcccct attcggccag cacagattgc tgggggtcag cggcaaggct gatatgctcc
    15121 agccgccaac gcttcaaccg cttgaaatcg gcgtagctca acgtagattc tgcgatgtgc
    15181 tcacagaacc tcaattcgat gtgcagccac tgctgggtgg acgtgaacag cttgtcttta
    15241 aaagcagtct taatggtatt ggcaacctca tcggctgcat gctcagctgc atcaacatcc
    15301 gggtcataca ggatgctgat atcgagggtg taagtgtcgt cagggccact acgcaccacc
    15361 tcggcacctt catccacgtc aaagaaaacg ccggtaatga gctccccatg gggcctcacc
    15421 gccttagcta tcttgtcggc gagcttggtt tcccgcgtca aacgacgttc gaactcgtca
    15481 gggaaagccg agcggcggta acgacttgcc aaccacattt ggaaaatagt gtgattctcg
    15541 gcggtcaaat gcacgtcgga acgtgggtaa aactcgttta gcttgagttt ttcgatggag
    15601 ctcttagctg ttgcctcgaa ttccgcccaa aaagtatcac cgcccacaaa ctcgacatgg
    15661 agcttgcggg cgttcttggc gtgggtgcag ttgccgtcct tgtctgtcac caagcaaccg
    15721 atgacgatct caatcttggg ctccccttcg ggggcctgtg caaggtcgca atcgtggctg
    15781 gcgacaatga ctacggtttg ttcagatcta gcggcatgac gaagccccaa agtggtgatg
    15841 gcatcttggc ttagcaaatg cccttgccgc catggggtat tccgattcca ttcagccacg
    15901 tttcatcctc aagcaaggtc gttggcagaa gggaggtcga agtcagcact agatggcgtc
    15961 ttcgtacgat tggcgaattg ggccgctatg cgttcacgtt gcgctgcttc gcgcttgtaa
    16021 atgcgcacca actgattcgc ggcttcccgc gccgactcgc cagcctgcgc aacctgtaac
    16081 agcgtccgac cattagcaaa cttacgcttt aacagggcag cgttaaccac gactccctca
    16141 taggccagca catcagcagc gtgcgcgaga tcctttaatt tggctacgtt cttatctgct
    16201 acaggttcgc cgttgagcca gttgtataca gactggcgcg atacatcgaa agtagttgcg
    16261 aggtcggaca ccgccggttt tagcacctcg cggatgcgag cgaggtcttc agccggagta
    16321 cgatcgacaa ctttgctcgt ggacgctatg tgaggtagca gctgagtact gcgctgcaga
    16381 cgctcaatcg actgtccacc accggtactg actctagtca gcccggcaac cagcacgccc
    16441 ctcatccacc ccacgaagcg aacgacggcg gccgatacac cagtagcggc cggtcggtca
    16501 gtgacccaac cgccataggt cattgcagta tccataattc tcccctttat gcccaagaga
    16561 cccgtgcgtg atcggtaact gtagcctcga aagatttgac gatttcgtca tgcagtgctg
    16621 tcaaatacgt atcaatcttc tcaagctgga aggcctcacg gcgaacaatg gccgcatccg
    16681 tatctacgat tgcgtgcagg ctctcacact gcgtgaaacg cggatcaatg gtgggagcca
    16741 agtcacttaa ctccatcggt agcccaataa atccatcccg aattaagaca cgcgacacga
    16801 gctgacctgc ggctgtcagg aaactggtct cactgaccga gtgctggagc tttccaccct
    16861 ccccgcgtag cagcgcatgc cctaacactt ctggaactag aaatgcccgt agactctcct
    16921 ccttcgaagg ctgaatcgca ttcaagtacc gcaagccaat gcgctccaca aagtcaaggc
    16981 caagcgcgtt gtgcagaatg cgaaggcctc ccagcaaagt ttcggagaat gtttcaaaag
    17041 tgtcgtagcg ggtggtctga aacgacaggg tgtttgtctc cagcataaac gacgaacgcc
    17101 catcaatgtc accaaataag taacgcgact gagaagccac agttggcgca gccatttgat
    17161 cttgctcagc attcccgaag ggaaacacga gccgctgaac aacttccttc ttgtagtcag
    17221 agaagtgcgt ttcgcgcatc ttggactgga tggccgggat gtaaccatcc aagtccagca
    17281 ccggattgaa ctgcacctgc gcaaccgtat agtataaagg cgcgctactc agcttagttc
    17341 ccatacaggt gtcctcgtga agtgtcttga tgctatataa tgctagcact ttacactatg
    17401 gttgacagta gaagcaatgc acgaaatagc accattgcat gcacttttac catctacaat
    17461 ttctcagcat ttatcagcgc taagggcaca gtgccactgt caggatgacc tgaatggtac
    17521 gcgaccagcg aagtcagccc ctcacctatc gcgctggcga tccaatcatg ctcttggttt
    17581 gtaatcgttt tcataacgca ccgtgctgat gcct
//
LOCUS       DAOPYX010000024        16830 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_3619_length_16830_cov_8.510700, whole genome shotgun sequence.
ACCESSION   DAOPYX010000024 DAOPYX010000000
VERSION     DAOPYX010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 16830)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16830)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16830
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3619_length_16830_cov_8.510700"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..180
                     /locus_tag="PKN55_02370"
     CDS             <1..180
                     /locus_tag="PKN55_02370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009119850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase Cbb3"
                     /protein_id="HNX70121.1"
                     /translation="PNLTDNIWLYGGTEKTIIETITNGRANQMPAWKDFLGDGKVHLL
                     TAYVWGLSNNKAAAQ"
     gene            497..1942
                     /gene="ccoG"
                     /locus_tag="PKN55_02375"
     CDS             497..1942
                     /gene="ccoG"
                     /locus_tag="PKN55_02375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011134725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase accessory protein CcoG"
                     /protein_id="HNX70122.1"
                     /translation="MSSDKKSIPIKPVPADNKADNKAEVVSLYAEHKKIHPRHVTGLF
                     ANMRVAMVWLTQLVYFGFPWMTWNGRQAVLFDLVNRKFYFFGITFFPQDFIYVAALLM
                     MCALGLFLWTTIAGRLWCGYACPQTVYTEVFLWFEHLIEGDRSKRIKLDAAPMSARKL
                     GLKTAKHTLWIAFSLITGFTLVAYFTPRTMLIDELLVWDLGPWETFWIFFYAFATYGM
                     AGFMREQVCKYMCPYARFQSVMFDADTLIISYDTERGEPRGTRKKGVDPASVGKGSCV
                     DCGICVQVCPTGIDIRNGLQYECIGCAACIDACDEVMDKMNYPRGLIRYTTENALEKH
                     YPESQIWTRIKRPRVIMYSLVLFTVAGIAITGLMMRQPMKVDILRDRASLVRETQDGL
                     LENTYNIRFINISENPQKLKLSVQGLPGIKLVAERDVFDVPPTGNVLVTLNVQAPPES
                     APKGSHKIQFVIKSLDDNAVNLEEPSSFIGE"
     gene            1950..2489
                     /locus_tag="PKN55_02380"
     CDS             1950..2489
                     /locus_tag="PKN55_02380"
                     /inference="COORDINATES: protein motif:HMM:NF017559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FixH family protein"
                     /protein_id="HNX70123.1"
                     /translation="MSQSTTPISTVPWYRQRWPWLLMLGPMVVVVAGLTTAAVAVRTQ
                     DALVNDDYYKRGKEINAELTRDNEAVRLKMGAQVMFGEGGRDVRVMLQSPVAAAPDKV
                     RLLMLHPTLSDRDQQVELVKSGEGFYTGKLTLPAAAHWFVRLEDISGRWRLQSQWKPA
                     EDNVVALSASASGGQAADN"
     gene            complement(2685..3062)
                     /locus_tag="PKN55_02385"
     CDS             complement(2685..3062)
                     /locus_tag="PKN55_02385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008952357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNX70124.1"
                     /translation="MKTAHDLVLAARAGITELTPSQAQAWQATCGALLIDVREPDEFA
                     AGHVPGAVNVPRGMLEFRIADLQPDGNAPVLVYCKTSGRAALSCQSLASLGYTQLRSV
                     AGGFDQWLAEGLPVEHATLPSFD"
     gene            complement(3183..3986)
                     /locus_tag="PKN55_02390"
     CDS             complement(3183..3986)
                     /locus_tag="PKN55_02390"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HNX70125.1"
                     /translation="MSWTLFNLLWTGALIGGLQGVTGAGGGIMAVPALVAVLGWGVVQ
                     AGPVALIGVAGSAALGAWDGFRHGLVRWRAALLMASAGVPASGLGAMLAQQLPGRVLL
                     ALFAAVMLWTAWRLLNKSGQTGDDEASLCPLHPETGRFVWPLRSMLLISGVGVLSGLM
                     TGLLGVGGGFIIVPALRRLSPLPMHSIAATSLLVIALVGAGGVGMATWHGVHPPVDVA
                     APFLLASLVGMLVGRRVSRRLSQAAVQRSFAWMVCAVAGYLLLKSAGLV"
     gene            complement(3983..4846)
                     /locus_tag="PKN55_02395"
     CDS             complement(3983..4846)
                     /locus_tag="PKN55_02395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008952063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNX70126.1"
                     /translation="MNPIIDSFYDPATWTVTHLVYDHPGGVAAIIDPVLDFDPRSGRT
                     RSDSAQQVLAAARALDLHVAWVLETHAHADHLSAAAWLKAQLGCSVGIGERIREVQHT
                     FAGLFNLGDAAPRDGAPFDRLFADGEIFALGQLSVEVLAVPGHTPADVAYRIGEHVFV
                     GDTLFMPDVGTARCDFPGGDAHQLYRSIRRLLALPESTQLMLCHDYPPAGRTEPAWMC
                     TVAAQRAGNIHVHDGVDEATFVERRLARDRTLDMPTLLLPSVQVNIRAGQLPPAEANG
                     VSYLKLPVNAL"
     gene            4863..5108
                     /locus_tag="PKN55_02400"
     CDS             4863..5108
                     /locus_tag="PKN55_02400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX70127.1"
                     /translation="MDEFIIYKSIMIAKSYADAQLMEMAMQNARWFQRALGAFTWPSV
                     ASLTKLSEARLARRAAADRAYKTKAAQQGQAAGVSSV"
     gene            complement(5105..6205)
                     /locus_tag="PKN55_02405"
     CDS             complement(5105..6205)
                     /locus_tag="PKN55_02405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014086360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX70128.1"
                     /translation="MSMLLPDPRLYPRVDPDNLLIRLAMALLTEREAGRALTRAEALK
                     DAIASQLAADDDSVLNAALRHAPSQEVYKAVWQAALDVVGQVGNERHAVIFALPVVLV
                     AGCKGRAELPERLSDVDALSQLLRQHGVIDAQAECFISGKLVHPDTLEGLSFSQLYRY
                     SRNLADAGRGLPVELPGDPAPVKDDCVLVRYLVGVAMREANGEMPVKLGGSMGAWGMP
                     LMQLLGEQLKTANVTLFPIPRVPNWLREAHRQGQAARQEVNLQVFASNIIRKLREYSL
                     PITAIAAAHDNGELRFTISTPGDDKNWAAHAWPLAPLDSVERIAEEFMGLMRECQVED
                     VRLVKEVQTDREHGIPFFVTVNDPAAQSHGLH"
     gene            complement(6145..6915)
                     /locus_tag="PKN55_02410"
     CDS             complement(6145..6915)
                     /locus_tag="PKN55_02410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX70129.1"
                     /translation="MFPAGLGRAGLPMWAAFYGDQWQKTGEKLAVSALTGQKLHNPCA
                     GSNWAFCLDGQTECPQINENSRRRTFFLLQRSNFQQLCLCPWHQGFPLHDSVSVLASP
                     AQAGLTQQGKHRKKSRKNKGGNFWQKKAREISPRFNLQRRNRGETIKKQLLHLNEYGL
                     SRPTVTLSTLFMLLGIIFVKNCRCRANKMFISEHQRYTGPATGNSAICPEHACTSGAA
                     DGICATITPILCPSSGTLPCRCCCLIPACTPVLTPITC"
     gene            7551..9125
                     /locus_tag="PKN55_02415"
     CDS             7551..9125
                     /locus_tag="PKN55_02415"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing methyl-accepting
                     chemotaxis protein"
                     /protein_id="HNX70130.1"
                     /translation="MKLNQPVTGREKPFTHGTIVTKTDLKGIITYANDAFVDMSGFSR
                     EELLGVNHNIVRHPDMPPTLFDDMWKTLRRDKPWQGLVKNRCKNGDHYWVSAFVVPIR
                     ERGQTTGYMSVRSPAPRDGIAKAEILHRQLGRDGKLKKSRGLRLSSTAKVLLGLLFIN
                     AVALTSATLDTPWARWGGVGAMLLGSLLAWLAYGRARQRYAHINHTFARIAEGVLTNP
                     LPTDRDDEIGLIESGLASMQVQVKVIVDDLLHDARLVESRAKKLNDSMAEMMRRFDSQ
                     ADSACAISAAIEQMSASITQVADNAKVAAEHTDSARELAESGETRMRVSHEGTQKTVH
                     IMQNAQGAIDQLHSAVGSIATVTETIHAIAEQTNLLALNAAIEAARAGEAGRGFAVVA
                     DEVRKLAERTSQSTGEIHALVSQVTAVAQSVVAAMGQVGEHTRHGEGQLDAAMAALQQ
                     IVAASIQVNDMVCGIAETNHQQSITSHDLVNRTVNVSEQLEASAQAAQQARSMIDDLL
                     RQAEKMARLARHFEAD"
     assembly_gap    9138..9237
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(9262..9999)
                     /gene="phbB"
                     /locus_tag="PKN55_02420"
     CDS             complement(9262..9999)
                     /gene="phbB"
                     /locus_tag="PKN55_02420"
                     /EC_number="1.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014087410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018454 - acetoacetyl-CoA reductase
                     activity [Evidence IEA];
                     GO_process: GO:0042619 - poly-hydroxybutyrate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetyl-CoA reductase"
                     /protein_id="HNX70131.1"
                     /translation="MTKKIALVTGGMGGIGTAICKALADAGCTVVTTYSKSGKEVQWL
                     ADMKEQGYAMHAYLCDVTDTDACAATVAQIAAEVGPVDILVNNAGITRDGTFKKMNKV
                     DWDMVMRTNLDSLFNLCKPTVDGMVERGWGRVINISSINGQKGQFGQTNYSAAKAGMH
                     GFSMALAQEVAKKGVTVNTVSPGYIGTDMVMAVPEEVRNKIIAGIPVGRLGKPEEIAG
                     LVTYLASDLAGFITGANLAINGGQHMM"
     gene            complement(10428..11306)
                     /gene="rsgA"
                     /locus_tag="PKN55_02425"
     CDS             complement(10428..11306)
                     /gene="rsgA"
                     /locus_tag="PKN55_02425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008952819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HNX70132.1"
                     /translation="MSQTCTGQIITSYGRRYIVETPEGVRYDCTTRGKKTDYACGDHV
                     DLLVQNAEQAVIERAQPRQSLLYRSDAFRSKLIAANVTQLAIVLAPVPTFSEELLNRC
                     LVAAEAGGIAPLIVLNKCDLPQAEAARARLAAWQALGYPLLPLSAREDVSALRARLQG
                     HTSVLVGQSGMGKSTITNALLPDACARVGEISTALDSGRHTTTHAQLYHLDADSHLID
                     SPGLQEFGLRHLHAPDLIHEFPELRPYIGQCRFHNCSHRVEPGCALIAAAQSGAVSAE
                     RLAFLQKLTDELSPRG"
     gene            complement(11303..12550)
                     /locus_tag="PKN55_02430"
     CDS             complement(11303..12550)
                     /locus_tag="PKN55_02430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008952817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HNX70133.1"
                     /translation="MSSPLTLLFVFALALSLALRLWLAARQHAHVRAHRQAVPAAFAG
                     QVSLADHQKAADYTCAKTRLTQLDALWDSAILLGLTLGGGIDALARLWAGWLADPLWQ
                     GVAVIASTLLLSGALSLPLSLYATFGVEARFGFNRSTPALYLADLAKSLALSALLGLP
                     VLALVLWLLAALGDQAWLAVWSVWVGLSLLLMWLFPTVIAPLFNRFQPLADQDLKTRI
                     EGLLARCGFASSGVFVMDGSRRSSHGNAYFTGFGRAKRIVFFDTLIKQLSPAQIEAVL
                     AHELGHFHHRHIVQRMFTSFALSLAVLAGMQWLLGWAGFQPALGVSHGTLATGLLLFL
                     LALPAFTFPLTPLTSLLSRRHEYQADAFAARHASPAELIAALVALYRDNAATLTPDPL
                     HSRFYDSHPPASLRIAHLKELSA"
     gene            12699..13244
                     /gene="orn"
                     /locus_tag="PKN55_02435"
     CDS             12699..13244
                     /gene="orn"
                     /locus_tag="PKN55_02435"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008952816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-exoribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HNX70134.1"
                     /translation="MAQDQNNLIWLDMEMTGLDPDNDRIIEMAVVITDSQLNTIAESP
                     VLVVHQSDAVLDGMDDWNKSTHGRSGLIDKVKASTLDEAAAQEQMLAFLREHVPGRAS
                     PMCGNSICQDRRFMARWMPALEAHFHYRNLDVSTLKELVKRWKPELAKGLRKHGKHEA
                     LADIYESIEEMKYYREHFIRL"
     gene            complement(13373..13705)
                     /locus_tag="PKN55_02440"
     CDS             complement(13373..13705)
                     /locus_tag="PKN55_02440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012696265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HNX70135.1"
                     /translation="MATTDSSPARIAVEVVYALPERQTLLALHVPVGATAQEAVALSG
                     LLRQHPELDLAQHKLGLFGKVSAADTVLREGDRVEIYRPLLADPKEVRRRRAAEGKAM
                     RKGGGEAG"
     gene            complement(13692..14129)
                     /locus_tag="PKN55_02445"
     CDS             complement(13692..14129)
                     /locus_tag="PKN55_02445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008953158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RatA family
                     toxin"
                     /protein_id="HNX70136.1"
                     /translation="MPVVEKNVLVAHTPAQMFHLVDDVERYPKFLPWCARGEVISRQG
                     PELVASLHIDYLKIRQHFTTRNYNHDDYTIDMTLVDGPFEQLSGGWRFIPLGDIGCKI
                     EFRLNYEFSSKILEKIIGPVFSHISGTLVDCFIREADRLYGDD"
     gene            14282..14731
                     /gene="smpB"
                     /locus_tag="PKN55_02450"
     CDS             14282..14731
                     /gene="smpB"
                     /locus_tag="PKN55_02450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003679566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HNX70137.1"
                     /translation="MSIIQNKKAFHDFFIEEKFEAGLVLEGWEVKSIRAGRVQLKESY
                     VIIKQGAIWLIGCHISPLPTASTHVHPDPTRIRKLLLKGGQIDKLIGKVERAGYTLAA
                     LDMHYRNGWVKLEIGLAKGKKQHDKRETEKNREWEREKQRLMKHNVR"
     gene            14739..15071
                     /locus_tag="PKN55_02455"
     CDS             14739..15071
                     /locus_tag="PKN55_02455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX70138.1"
                     /translation="MHRNATFPVLLITAGLLWFLHSTALLPNAHSLVAIGLAVAGAAM
                     LLLDGLNKSTLVSCPMLMYAGLVVYGREHFALPFSVWASCGMVLLGLLLLAARSSAVP
                     ERSRRRPD"
     gene            15127..16704
                     /gene="guaA"
                     /locus_tag="PKN55_02460"
     CDS             15127..16704
                     /gene="guaA"
                     /locus_tag="PKN55_02460"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017506983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HNX70139.1"
                     /translation="MDKILILDFGSQVAQLIARRVREAHVYCELHPYDMPIDAIRAFN
                     PKAIILSGGPNSVYESDYQADPALFSLGVPVLGICYGMQWMAQTLGGKVESGDTREFG
                     YAEIRARGHSKLFNGLQDRSNAEGHGLLDVWMSHGDKVTALPDGFTLIAETPSCPIAA
                     MADEARGFYAVQFHPEVTHTKRGTDILHRFVLSIAGAAPSWTMPNYIDEAVKKIREQV
                     GDEEVILGLSGGVDSSVAAALIHRAIGEQLTCVFVDHGLLRLNEGQMVMDMFARNLGV
                     KVVHVDASAEFMGKLAGETDPEKKRKIIGAEFVEVFQAESKKLTNAKWLAQGTIYPDV
                     IESAGAKTKKAHTIKSHHNVGGLPETLNLKLLEPLRELFKDEVRELGVALGLPHEMVY
                     RHPFPGPGLGVRILGEVKAEYASLLQRADAIFIEELRNTIDEASGKSWYDLTSQAFAV
                     FLPVKSVGVMGDGRTYEWVVALRAVVTSDFMTAHWAELPYSLLGRTSNRIINEVRGIN
                     RVVYDVSGKPPATIEWE"
BASE COUNT         3345 a         5183 c         5208 g         2994 t
ORIGIN      
        1 ccgaacctga ccgacaacat ctggctgtat ggcggcactg aaaagaccat catcgagacc
       61 atcaccaatg gtcgcgccaa ccagatgccg gcgtggaaag acttcctggg cgacggcaag
      121 gtgcacctgc tgaccgccta tgtgtggggt ctgtccaaca acaaggccgc tgctcagtaa
      181 tcgctgaggc ctgttgagat gcgcaacact gcccgcttcg gcgggcagtg tcgtttctgc
      241 ctgttggcgc gggccggatt tgacctgtgt caacaagtgg aaaacttggg ttcactaatt
      301 tcacgcagtc actatagatt ttgatagaat aaatatagcg ccaaatatcc tttgcgtatg
      361 gcgtaaactt cctgcttgtc gagctgtctg gcaagctggc tctgctgaat gcagagggtt
      421 tagccaagca gtggcctgcc gcttcgcgcc ggtctgctgc gactgtttgg ctaaaccttt
      481 ctcaggaacc acgagtatgt cgagcgacaa aaagtccatc ccgatcaagc ccgtgcctgc
      541 cgacaataag gcagacaaca aggctgaagt cgtctctctg tacgccgagc acaagaaaat
      601 ccacccgcgt cacgtcaccg gcctgtttgc caatatgcgc gtggccatgg tctggctgac
      661 ccaattggtg tatttcggct ttccctggat gacctggaac ggccgccagg cggtgctgtt
      721 tgatctggtc aaccgcaaat tctacttttt tggcatcacc ttcttcccgc aggatttcat
      781 ctacgttgcc gccctgctga tgatgtgtgc gctgggcttg ttcctgtgga ccaccattgc
      841 cggccgctta tggtgtggct acgcctgtcc gcagacagtg tacaccgaag tgtttttgtg
      901 gtttgagcac ctgatcgaag gcgaccgcag caagcgcatc aagctggatg cagcaccgat
      961 gtcggcgcgc aaactgggtt tgaagaccgc caagcacacg ctgtggattg ccttctcgct
     1021 gatcaccggc tttaccctgg tggcctactt taccccgcgc accatgctga tcgacgagtt
     1081 gctggtgtgg gatctgggcc cgtgggaaac cttctggatc ttcttctacg cctttgccac
     1141 ctatggcatg gccggcttca tgcgcgagca agtgtgcaaa tacatgtgcc cgtacgcccg
     1201 cttccaaagc gtgatgtttg atgctgacac cctgatcatc tcctacgaca ccgaacgcgg
     1261 tgagccgcgc ggcacgcgca agaagggcgt ggacccggcc agcgtcggca agggcagctg
     1321 tgtggactgc ggcatctgcg tgcaggtgtg cccgaccggc atcgacatcc gcaacggcct
     1381 gcaatacgag tgcatcggct gcgccgcctg tatcgacgcc tgcgacgagg tgatggacaa
     1441 gatgaactac ccgcgcggcc tgatccgtta caccacggaa aacgcgctgg aaaaacacta
     1501 tccggaaagc cagatctgga cccgcatcaa gcgtccgcgg gtgatcatgt attcgctggt
     1561 gctgttcacc gtcgccggta tcgccatcac cggcctgatg atgcgccagc cgatgaaggt
     1621 ggatatcctg cgcgaccgcg cctcgctggt gcgtgaaacc caggatggcc tgctggaaaa
     1681 tacgtacaat atccgcttta tcaatatttc cgagaacccg cagaagctga aactgtctgt
     1741 gcaaggtctg cctggcatca agctggtggc cgagcgagat gtgtttgacg tgccgcccac
     1801 gggcaatgtg ctggtgaccc tgaacgttca ggcaccgccg gagagcgcgc ccaagggcag
     1861 tcacaagatt cagtttgtga tcaagtccct ggatgacaat gcggtcaatc tggaagagcc
     1921 cagcagcttt attggagagt aagcgcatca tgagccaatc gaccaccccg atttccaccg
     1981 tgccctggta tcgtcaacgc tggccttggt tgctgatgct cggcccgatg gtggtggtgg
     2041 tcgctggctt gaccacggct gctgtggccg tgcgcaccca ggatgcactg gtgaacgacg
     2101 actattacaa acgcggcaag gaaatcaatg ccgagctcac ccgcgacaac gaagctgtcc
     2161 ggctgaaaat gggtgcacaa gtgatgtttg gcgaaggtgg ccgtgatgtg cgggtgatgt
     2221 tgcaaagccc ggtggccgct gcgccggaca aagtgcggct gctgatgctg catcccaccc
     2281 tgtcggatcg tgatcagcaa gtcgagctgg tcaagagtgg cgaaggcttt tacacgggca
     2341 agctgacttt gcctgcagcc gcgcactggt ttgtccggct ggaagatatc agcggccgct
     2401 ggcgcttgca aagccaatgg aagcccgctg aagacaatgt ggttgccttg tcggccagcg
     2461 cctcgggcgg tcaggcagcg gacaactgat caactgcggt aattcacttg catgccgtgt
     2521 tcgacggcat accccacccc gcttcggcgg ggtttttcat gtctattgag tgctgaggtg
     2581 gcacgattga ccaaatgcag cacagcactg ctggccaagt gtgcgaccgc agcagatata
     2641 acgggcgcca tgcaacaacg ccaggagggg tttctcagca gcgcttaatc gaacgacggc
     2701 agcgtcgcgt gctccaccgg caagccctcc gccagccact gatcaaaccc gcctgccacc
     2761 gagcgcagct gggtatagcc caggctggcc agtgactgac aagacagcgc cgcgcggccg
     2821 ctggttttgc agtacaccag cacaggcgcg ttgccatcgg gctgtaaatc agcgatgcga
     2881 aactccagca tgccgcgcgg cacattcacc gcaccgggca catggccggc ggcaaattcg
     2941 tccggctcgc gcacatcaat cagcaaagcg ccgcaggtgg cctgccaggc ttgcgcctgc
     3001 gacggggtga gttcggtgat gccggcgcgc gcggccagca ccagatcgtg tgcggttttc
     3061 atcagtcaaa cactcctaaa taaaaaagca tgctggccag tcgcctggca cagcatgtca
     3121 gcagggtgaa gcaagaaaca cccggagaaa accctgagag acaacccgcg gcgggcgcag
     3181 actcacacca gcccggcgct tttcagcaat aaatagccgg ctactgcaca caccatccag
     3241 gcaaaactgc gctgcacggc ggcctgcgac aaacggcgcg acacccgtcg gcccaccagc
     3301 atgccgacga gactggccag caaaaagggc gccgcaacat cgacaggagg atgcacgccg
     3361 tgccaggtgg ccatgcccac gccgccagcg cccaccaggg caatcaccag cagcgaggtg
     3421 gcggcaatgc tgtgcatcgg caaagggctg agccggcgca aggctgggac aatgataaaa
     3481 ccgccgccca cgcccagcag gccggtcatc agcccggaca gcacgcccac accgctgatc
     3541 agcaacatgc tgcgcaaggg ccagacaaag cgcccggttt ccgggtgcag cgggcatagc
     3601 gatgcttcgt catcgccggt ttggcccgat ttgttgagca aacgccaggc ggtccacagc
     3661 atcaccgcgg caaacagcgc cagcaacaca cgccccggca attgctgcgc caacatggcg
     3721 cccaggccgg acgccggcac gccggcgctg gccatcagca aggcggcgcg ccagcgcacc
     3781 aggccatgcc gaaagccatc ccaggcgccc agcgcggcgc tgccagccac gccaatcaag
     3841 gccaccggcc cggcctgcac cacaccccag cccagcaccg ccaccaaggc cggcaccgcc
     3901 atgatgccgc cgcccgcgcc agtgacgcct tgcaggccgc caatcagcgc gccggtccac
     3961 agcagattaa acagcgtcca gctcacagcg cattgaccgg cagcttgaga tagctgacac
     4021 cattggcctc ggccggcggc aactggccgg cgcggatgtt cacctgcacc gagggcagca
     4081 acaaggtggg catgtccagc gtgcggtcgc gcgccagccg gcgctcgaca aacgtcgcct
     4141 cgtccacgcc gtcatgcaca tggatattgc cggcgcgctg tgccgccacc gtgcacatcc
     4201 aggcgggttc ggtgcggcca gccggcgggt agtcgtggca cagcatcaac tgggtggatt
     4261 ccggcagggc cagcagccgg cggatcgagc ggtataactg atgggcgtcg ccgcccggaa
     4321 aatcacagcg cgcagtgccg acatccggca tgaacagggt atcgccgaca aacacatgct
     4381 cgccaatccg gtaggcgaca tcggccgggg tgtggccggg cacggccagc acttccacgc
     4441 tcagctggcc cagggcaaaa atttcgccat cggcaaacag ccggtcaaac ggcgcgccat
     4501 cgcgcggggc ggcgtcgccc agattgaaca ggccggcaaa ggtgtgctgc acttcgcgga
     4561 tgcgctcgcc aatgcccacc gagcagccca gctgcgcttt cagccaggcg gcggcggata
     4621 aatggtcagc gtgggcgtgg gtttccagca cccaggccac gtgcaaatcc agcgcgcgcg
     4681 cagcggccag cacctgttgc gccgaatcac tgcgcgtgcg gcccgagcga gggtcaaaat
     4741 ccagcaccgg gtcgataatc gccgccacgc cgccagggtg gtcgtacacc aagtgggtga
     4801 cggtccaggt ggcagggtcg taaaaactgt cgatgatcgg gttcatgccg ggctccgggc
     4861 gagtggatga atttattata tacaaaagta taatgattgc aaaatcgtat gcagatgctc
     4921 agctgatgga gatggccatg caaaacgccc gctggtttca gcgggcgttg ggtgcgttca
     4981 cttggccaag tgttgcatcg cttaccaaac tcagtgaagc gcgcctggca aggcgtgcgg
     5041 ccgcagaccg tgcttataag acaaaggccg cgcaacaagg ccaggcggct ggtgtgagca
     5101 gcgtttaatg caggccgtgc gattgcgccg ccgggtcgtt gacggtgaca aaaaacggaa
     5161 tgccatgctc gcggtcggtt tgcacttctt tcaccagccg cacgtcttcc acctggcatt
     5221 cgcgcatcag gcccatgaat tcttcggcaa tgcgctccac cgaatccagc ggcgccagcg
     5281 gccaggcgtg cgccgcccag tttttgtcgt cgcccggggt gctgatggta aagcgcagct
     5341 cgccattgtc gtgggcggcg gcgatggcgg tgatcggcag cgaatattca cgcagcttgc
     5401 ggatgatatt gctggcgaac acttgcagat tgacttcctg gcgggcggcc tggccctggc
     5461 ggtgcgcctc gcgcagccag ttgggcacgc gcgggatggg gaacagcgtc acattggcgg
     5521 ttttcagctg ctcgcccagc aattgcatca gcggcatgcc ccaggcgccc atgctgccgc
     5581 ccagtttcac cggcatttcg ccattggcct cgcgcatggc cacgcccacc agatagcgca
     5641 ccagcacgca atcgtccttc accggcgccg ggtcgcccgg cagctccacc ggcaggccgc
     5701 ggccggcgtc ggccagattg cggctgtagc ggtacagctg gctgaagctc agcccttcca
     5761 gcgtgtccgg gtgcaccagc ttgcccgaga taaagcattc cgcctgggcg tcgatcacgc
     5821 cgtgctggcg cagcagctgg ctgagcgcgt ccacatcgct cagccgctcg ggcaactcgg
     5881 cgcggccctt gcagccggcc accagcacca ccggcaaggc aaaaatcacc gcatggcgct
     5941 cgttgcccac ctgacccacc acgtccagcg cagcttgcca gacggcttta tacacttctt
     6001 gcgacggcgc atggcgcagc gcggcgttca gcacgctgtc gtcgtcggca gccagttgcg
     6061 aggcgatggc gtctttcagc gcttcggcgc gggtcagcgc acggccggcc tcgcgctcgg
     6121 tcagcaaggc catggccagg cgaatcaaca ggttatcggg gtcaacacgg gggtacaggc
     6181 ggggatcagg cagcaacatc gacatgggag ggttccgcta ctagggcaca aaatcggcgt
     6241 tattgtcgca cagatgccgt ctgctgcgcc gctcgtgcat gcgtgctcag ggcagattgc
     6301 gctattgccg gtggcagggc cggtgtaccg ctgatgttcg ctaatgaaca tcttatttgc
     6361 cctacagcga caatttttta caaaaatgat gcctagcaac atgaagagcg ttgacagggt
     6421 cacggtcggt ctggataatc cgtactcgtt gagatgcagc aattgcttct tgatagtttc
     6481 tcctctattt ctcctttgta gattgaagcg cgggcttatc tcccgcgctt ttttttgcca
     6541 aaaattcccg cccttgtttt ttctggattt ttttctgtgt ttaccttgct gggtaaggcc
     6601 tgcttgcgca ggtgacgcca gcactgagac gctgtcatgt agggggaaac cctgatgcca
     6661 aggacagaga cacagttgct ggaaattgct gcgctgcagc aaaaaaaatg ttcgtcttcg
     6721 agaattttcg ttgatttgtg gacattctgt ttggccatcc agacaaaatg cccagtttga
     6781 tccagcgcaa ggattgtgca gtttttgccc tgtcagggct gaaacagcca gtttttcacc
     6841 tgttttttgc cattgatcgc cgtagaatgc tgcccacatc ggcagacctg cccggcccaa
     6901 tccggccgga aacacaccac gccgccagcc atggccggca cggtgcgcag catgtgccgc
     6961 tgcattgcag cggcaaacgg cccatctctt gtggtggccc gcgtctgcag gatgcgctct
     7021 cccgggtttt gggtgttgga gagcctgtcg cgcggcatgc atgggtagtt gggggcaggc
     7081 cgcgagatga caagggatca ggcaaatgac ggggcaggtg tgggggcatc cgccccgtca
     7141 tgattcctcg gtgcgtatac cggggccgag gctgattcca gcgcgtattc gttccagtat
     7201 tgccggcggg ttctgaccgt cggtcatcct ccttgatcag atgttggcag tatgttgtga
     7261 gtggccttgt gccagagcgg cagcatggcg cacgcctgtg gccaagcagg ccatggcgtt
     7321 cgcagcatgc ccgcgttgcc tggctgtgcg gggcatggcc ccgatattgc atagagactc
     7381 cagcgcgcca gctgatgcgg gctgaatatc atgcagggca acagtgttgc agcgcccgct
     7441 tgtcgggctc ggcgccgcac ccggatggcg gcaaaaaaat gaatccgcgc cacgcccaac
     7501 ctgcacacaa gacaggcggc gcgcacagta aagccagggg tagccagggg atgaagttga
     7561 atcaacccgt caccgggcgt gaaaaaccgt tcacgcacgg gacgattgtc accaaaaccg
     7621 atttgaaggg gatcatcacc tacgccaatg atgcgtttgt ggacatgtcc ggcttcagtc
     7681 gcgaagagct gctgggggtc aatcacaata ttgtgcggca ccccgatatg ccacccacgc
     7741 tgtttgacga tatgtggaaa accctgcgtc gtgataaacc atggcaaggg ctggtgaaaa
     7801 accgctgcaa gaacggcgac cattactggg tatcggcgtt tgtggtgccc attcgcgaac
     7861 gtggacagac cacagggtac atgtcggtac gttctccggc gccgcgtgac ggcattgcca
     7921 aggcggaaat cctgcatcgc cagctggggc gcgacggcaa gctgaaaaaa tcgcgcggcc
     7981 tgcgcttaag ctccaccgcc aaggtgttgc tgggcctgct gttcatcaac gctgtggcac
     8041 tgaccagcgc cacgctggat acgccatggg cgcgctgggg cggcgtgggc gccatgctgc
     8101 tgggctcgct gctggcctgg ctggcctatg gccgcgcgcg ccagcgctat gcccacatca
     8161 atcacacctt tgcccgcatt gccgaaggcg tgctgaccaa ccccttgccc accgaccgcg
     8221 acgacgaaat cggcctgatt gaatccggcc tggcgtcgat gcaggtgcag gtaaaagtga
     8281 ttgtcgatga cttgctgcac gatgctcgct tggtagaaag ccgcgccaaa aagctgaacg
     8341 acagcatggc cgagatgatg cgccgctttg acagccaggc agacagtgcc tgcgccattt
     8401 ctgcggcgat tgagcagatg agcgcctcca tcacccaagt ggccgacaac gccaaggtgg
     8461 cagccgagca caccgacagc gcgcgcgaac tggcggaaag cggtgaaacc cgcatgcgcg
     8521 tctcgcacga aggcacgcag aaaaccgtcc acatcatgca aaacgcccaa ggcgccattg
     8581 accagttgca cagcgcggta ggcagcattg ccacggtgac cgaaaccatt cacgccattg
     8641 ccgagcaaac caatctcttg gcgctgaacg cggcgattga ggccgcccgc gccggcgagg
     8701 ccgggcgcgg ttttgccgtg gtggccgatg aggtgcgcaa gctggccgag cgcaccagcc
     8761 agagtaccgg cgagattcac gccctggtca gccaggtgac ggcggtggcg caatcggtag
     8821 tcgcagccat gggccaggtg ggcgagcata cccgccacgg tgaaggccag ctggatgccg
     8881 ccatggcggc gctgcaacag attgtcgcgg ccagtattca ggtaaatgat atggtgtgcg
     8941 gcattgccga aaccaaccat cagcagtcga ttacctcgca tgacctggtc aaccgcacag
     9001 tgaatgtcag cgaacagctg gaggcttcgg cgcaggcggc gcagcaggcg cgtagcatga
     9061 ttgatgattt actgcgccag gccgaaaaaa tggctcgatt agcgcggcat ttcgaggcgg
     9121 attgagcgct gcggctgnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnntca
     9241 caccatcacg ccaaacgccg cttacatcat gtgctggccg ccgttgatcg ccaggttggc
     9301 gccagtgatg aaaccggcca gatccgaagc cagataggtc accaggccag cgatttcttc
     9361 cggcttgccc agccggccga ccggaatgcc ggcgatgatc ttgttgcgca cctcttccgg
     9421 caccgccatc accatatcgg tgccgatata gcccggcgac acggtgttga cggtgacgcc
     9481 cttcttggcc acttcctgcg ccagcgccat ggaaaaacca tgcatgccgg ccttggcggc
     9541 ggagtagttg gtctggccga actggccttt ctggccgttg atcgacgaga tattgatcac
     9601 ccgaccccag ccgcgctcga ccatgccgtc cacggtgggt ttgcacagat tgaacagcga
     9661 atccaggttg gtgcgcatca ccatgtccca gtccactttg ttcattttct tgaaggtgcc
     9721 gtcacgggtg atgccggcgt tgttgaccag aatgtccacc gggcccactt cggcggcaat
     9781 ttgcgccacg gtggcggcgc aggcgtcggt gtcggtcacg tcgcacagat aggcgtgcat
     9841 ggcgtaaccc tgctctttca tgtcggccag ccattgcact tccttgccgg acttgctgta
     9901 ggtggtgacc acggtgcagc cggcatcagc cagcgccttg caaatggcgg tgccaatgcc
     9961 gcccatccca ccggtgacca gtgcgatttt tttcgtcatg ggttctctct ccgtctgtgt
    10021 ctgtgtgttc tgcgcgcgcc gtggcaaaac atggcgcgcg gcgcgcctgc gcggcaaggc
    10081 ctggcgcagg cgtcagtgcg gatgttggcg gcagggtctg cgccctgtct tcgccatgcg
    10141 catcgcggcg ctgaccctat ggttatagca ttaaaatttt gcttgagtgt gacgacttgt
    10201 cgtgcggcgc agcattaacc cgtgcgtaaa ccgtgtgctg acgtgaaatt tttcgccaga
    10261 tttccagtaa aacagcaatc atctgccaga aaaatgccag caaggccgat tcacctgggg
    10321 gttttacgaa aaactaccta gtagacaaga atatgctgcg tcgccgcagg cggggcgggg
    10381 tttccgcgtg cgtcagcacc gcaaaaaccc gccagagagg gggcggctca gccgcgtggg
    10441 gacagctcgt cggtgagctt ttgcaagaac gccagccgtt cggccgacac cgcgccggac
    10501 tgggcggcgg caatcagcgc gcagccaggc tcgacgcggt ggctgcaatt atgaaaacgg
    10561 cactggccga tataggggcg cagttcggga aactcatgaa tcaggtcggg cgcgtgcagg
    10621 tggcgcaggc cgaattcctg caagccgggc gagtcgatca aatggctgtc ggcgtccagg
    10681 tgatacagct gggcgtgggt ggtggtgtgg cggccagaat ccagcgcggt ggaaatctcg
    10741 cccacccgcg cgcaggcgtc tggcagcaag gcgttggtga tggtggattt gcccatgccg
    10801 gattgcccga ccagcacgct ggtgtggcct tgcaggcgcg cgcgcagcgc gctgacgtct
    10861 tcacgcgcgg acagcggcag cagcggatag cccagcgcct gccaggccgc cagccgcgcg
    10921 cgcgccgcct cggcctgtgg caggtcgcat ttgttcagca cgatcagcgg agcaatgccc
    10981 cccgcctcgg ccgccaccag gcagcggttc agcagctctt cgctaaaagt cggcactggg
    11041 gccagcacaa tcgccagctg ggtgacattg gcggcgatca gcttgctgcg aaaagcgtct
    11101 gagcggtaca gcaggctctg gcgcggctgc gcgcgctcga tcaccgcctg ttcggcgttt
    11161 tgcaccagca aatccacatg gtcgccgcag gcgtagtcgg tttttttgcc gcgcgtggtg
    11221 cagtcgtagc gcaccccttc cggggtttcc acgatgtaac ggcggccata gctggtgatg
    11281 atctggccgg tgcaagtctg gctcatgcgg acaattcttt caggtgggca atgcgcaggc
    11341 tggccggcgg atgcgaatca taaaaccgcg aatgcagcgg gtcgggggtc agcgtggcgg
    11401 cgttgtcgcg gtacaaggcc accagcgcgg caatcagctc ggcaggcgag gcatgccgcg
    11461 cggcaaaggc gtcggcctgg tattcgtggc ggcgcgataa aaggctggtc agcggggtga
    11521 gcggaaaggt aaaagccggc agcgccagca agaacagcaa taagccagtg gccagcgtgc
    11581 cgtggctgac gcccagcgcc ggctgaaagc ccgcccagcc cagcagccac tgcatgccgg
    11641 ccagcaccgc cagcgacaag gcaaagctgg tgaacatgcg ctgcacaata tggcgatgat
    11701 gaaaatgccc cagttcgtgc gccagcaccg cttcgatttg cgccgggctg agctgtttga
    11761 tcaaggtgtc aaaaaacaca atgcgcttgg cgcggccaaa gccggtgaaa tacgcattgc
    11821 cgtggctgga gcggcgcgag ccgtccatca cgaacacgcc gctggaggca aagccgcagc
    11881 gcgccagcaa gccttcgatg cgggttttca aatcctggtc ggccagcggc tgaaagcggt
    11941 tgaacaaggg ggcaatcacc gtgggaaaca gccacatcag caacaggctc agccccaccc
    12001 acaccgacca caccgccagc caggcctgat cgcccagcgc cgccagcagc cacaacacca
    12061 gcgccagcac cggcaggcca agcagcgccg acagcgccag cgatttggcc aggtcggcca
    12121 ggtagagcgc cggcgtgctg cggttaaagc caaagcgcgc ctccacgcca aaggtggcgt
    12181 acaggctcag cggcaggctg agcgcgccgc tgagcagcaa ggtggaggcg atcaccgcca
    12241 cgccctgcca cagcgggtcg gccagccagc cggcccacag ccgcgccagc gcgtcgatgc
    12301 cgccgcccag cgtcaggccc agcaggatgg cgctgtccca cagcgcgtca agctgcgtca
    12361 gccgggtttt ggcgcaggtg taatccgccg ctttttggtg gtcggccagc gacacctggc
    12421 cggcaaaagc cgccggcacg gcctggcgat gggcgcgcac atgggcatgc tggcgcgcgg
    12481 ccagccacag ccgcaaggcc agactcaggg caagggcaaa aacaaatagc agggttagcg
    12541 gtgagctcat gggcagggcc ggatggagtg aagacggcag gatggactgc gcgggcgcgc
    12601 caacgttcgc tgccgggcgc gacgccaggt cggcgaacgc ggcaagttca tgcgacaata
    12661 accgtcttta tcagagtcaa cagaaacgaa cgcgcattat ggcacaggat caaaacaacc
    12721 tcatctggct ggacatggaa atgaccgggc tggatccgga caacgaccgc attattgaaa
    12781 tggccgtggt gattactgac agccagctca acaccattgc cgaatcgccg gtgctggtgg
    12841 tgcatcagtc cgacgccgtg ctggacggca tggacgactg gaacaaatcc acccacggcc
    12901 gctccggtct gatcgacaag gtcaaggcct cgacgctgga cgaagccgcc gcacaagagc
    12961 agatgctggc gtttttgcgc gagcacgtgc ccggccgcgc ctcgccgatg tgcggcaact
    13021 cgatttgcca ggatcgccgc tttatggcgc gctggatgcc ggcgctggaa gcgcattttc
    13081 actaccgcaa tctggacgtc tccacgctga aagagctggt caagcgctgg aagccggagc
    13141 tggccaaggg cctgcgcaag catggcaagc acgaggcgct ggcggatatt tatgaatcca
    13201 tcgaagaaat gaagtactac cgcgagcact tcatccggct gtaacagcct ggcctccttg
    13261 cgctgcgcgt gttgtcagcg gcgattcgcc cggcctgcgc gcctgtggca tgggcggcat
    13321 ctgcctgcgc cacggcagtg ccgggccacg gcgcctgctc agttggaagg cgtcagcccg
    13381 cctcgccgcc gcccttgcgc atggccttgc cctcggccgc gcggcggcgg cgcacttctt
    13441 tggggtcggc cagcagcggg cggtagattt ctacccggtc gccttcgcgc aagaccgtgt
    13501 cggcggcgct gactttgcca aacaggccca gcttgtgttg ggccagatca agctccgggt
    13561 gttggcgcag caggccgctc agcgccaccg cctcttgcgc agtggcgccc accggcacat
    13621 gcagcgccag cagggtttgc cgctcgggca gcgcatacac cacctcgacg gcgatgcgcg
    13681 ccgggctgct gtcagtcgtc gccatacagt cgatccgcct cacggataaa acaatccacc
    13741 agcgtgccag agatatggct gaacaccggg ccgatgattt tttccagaat cttgctggag
    13801 aattcgtagt tcagccggaa ttcaatcttg cagccaatat cgcccagcgg gataaagcgc
    13861 cagccgccgc tgagctgctc gaacgggccg tccaccagcg tcatgtcgat ggtgtagtcg
    13921 tcgtggttgt aattgcgcgt ggtgaagtgc tgacggattt tcaggtaatc aatgtgcagg
    13981 ctggccacca gctcgggccc ctggcgagaa atcacctcgc cgcgcgcgca ccagggcagg
    14041 aacttcgggt agcgctccac gtcgtccacc agatggaaca tctgcgccgg ggtatgggcc
    14101 accagcacgt ttttttctac aacaggcata ctcagctcgg ccaacatgag ggtaaagatg
    14161 accagcgcca ggcgccggca gcggcattgt accccggccg gccggtcaag cccatgaatt
    14221 tgcgccagtt tggcccggcg cgatgatctg ctaaaatgac cgattcaccc acgggggcgt
    14281 catgagcatt atccagaaca aaaaagcctt ccatgatttc ttcattgaag agaaattcga
    14341 agccggcctg gtgctggaag gctgggaggt caaatccatc cgcgccggtc gggtgcagct
    14401 gaaagaatcc tatgtgatca tcaagcaagg cgccatctgg ctgattggct gccatatttc
    14461 gccgctgccc accgcctcca cccacgtcca ccccgacccc acccgcatcc gcaagctctt
    14521 gctcaagggc gggcagatcg acaagctgat cggcaaggtg gagcgcgccg gctacacgct
    14581 ggcggcgctg gacatgcatt accgcaatgg ctgggtcaag ctggaaatcg gcctggccaa
    14641 gggcaaaaaa cagcatgaca agcgcgaaac cgagaaaaac cgcgagtggg agcgggaaaa
    14701 acaacgcctg atgaagcaca acgtgcgctg agcccgccat gcaccgcaac gcgacttttc
    14761 ctgttttgct gatcaccgcc ggcctgctgt ggtttttgca cagcaccgcg ctgttgccca
    14821 acgcccacag cctggtggcc atcggcctgg cggtggcggg cgcggccatg ctgctgctgg
    14881 acggcctgaa caaatccacc ctggtcagct gcccgatgct gatgtacgcc ggcctggtgg
    14941 tgtacgggcg cgagcatttt gccctgccgt tcagcgtgtg ggcctcgtgc ggcatggtgc
    15001 tgctgggcct gctgctgttg gccgcgcgca gcagcgccgt gcccgagcgc agccgccgac
    15061 gccccgattg aatcgtttga ctgtatcttg actgtatcgc cgctacgccg gcccggaaag
    15121 accctcatgg acaaaatcct cattctcgac tttggctcgc aagtggcgca gctgattgcc
    15181 cgccgcgtgc gcgaggcgca tgtctattgc gaactgcacc cctacgacat gcccatcgac
    15241 gccatccgcg cctttaaccc caaggcgatc atcctctcgg gcggccccaa ctcggtgtac
    15301 gaatccgact accaggccga cccggcgctg tttagcctgg gcgtgccggt gctgggcatc
    15361 tgctacggca tgcaatggat ggcgcaaacc ctgggcggca aggtggaatc cggcgacacc
    15421 cgcgaattcg gctacgccga aatccgcgcc cgtggccact ccaagctgtt caacggcctg
    15481 caagaccgca gcaacgccga aggccatggc ctgctggacg tgtggatgag ccacggcgac
    15541 aaagtcaccg ccctgcccga cggcttcacc ctgattgccg aaactccgtc ctgcccgatt
    15601 gccgccatgg ccgacgaggc gcgtggtttt tacgccgtgc agttccaccc ggaagtcacc
    15661 cacaccaagc gcggcacgga tattctgcac cgctttgtgc tgagcatcgc cggcgccgcg
    15721 ccgtcgtgga ccatgcccaa ctacatcgac gaagcggtga aaaaaatccg cgagcaagtg
    15781 ggcgacgaag aagtgattct tggcctgtcc ggcggcgtgg acagctcggt ggccgccgcg
    15841 ctgattcacc gcgccatcgg cgagcaactc acctgcgtgt ttgtcgatca cggcctgctg
    15901 cgcctgaacg aaggccagat ggtgatggac atgttcgccc gcaacctggg cgtgaaagtg
    15961 gtgcacgttg acgccagcgc cgagttcatg ggcaagctgg ccggcgaaac cgacccggag
    16021 aaaaagcgca agatcatcgg cgccgaattc gtcgaagtgt tccaggccga atcgaaaaag
    16081 ctgaccaacg ccaaatggct ggcgcaaggc accatctacc cggacgtgat cgaatcggcc
    16141 ggcgccaaaa ccaaaaaagc ccacaccatc aagagccacc acaatgtcgg cggcctgccg
    16201 gaaacgctca acctgaaact gctggagccg ctgcgcgagc tgttcaagga cgaagtgcgc
    16261 gagctgggcg tggcgctggg cctgccgcat gaaatggtct accgccaccc cttccctggc
    16321 cccggcctgg gcgtgcgcat cctcggcgag gtcaaagccg aatacgccag cctgctgcaa
    16381 cgcgccgacg cgatttttat cgaagaactg cgcaacacca tcgacgaagc cagcggcaag
    16441 agctggtacg acctcaccag ccaggcgttt gccgtgttcc tgccggtgaa atcggtgggc
    16501 gtgatgggcg acggccgcac ctacgaatgg gtggtggcgc tgcgcgcggt ggtcaccagc
    16561 gacttcatga ccgcccactg ggccgaactg ccctacagcc tgctgggccg gacttccaac
    16621 cggattatca atgaagtgcg cgggattaac cgcgtggtgt acgacgtgtc ggggaagccg
    16681 ccggcgacga ttgagtggga gtgagttggc gtctggcatc gactggcgcc gagtagcatg
    16741 aaaacacact taagcccgcg tcacagcggg ctttttgtcc tggtcaagtt ttttcgaaca
    16801 ccccgatagg gagtcagccg ctcaagcctg
//
LOCUS       DAOPYX010000025        16658 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_3671_length_16658_cov_8.697946, whole genome shotgun sequence.
ACCESSION   DAOPYX010000025 DAOPYX010000000
VERSION     DAOPYX010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 16658)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16658)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16658
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3671_length_16658_cov_8.697946"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..194
                     /locus_tag="PKN55_02465"
     CDS             <1..194
                     /locus_tag="PKN55_02465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX70140.1"
                     /translation="GLRVTLKRRSKEMLKKLSFPRRRESRVLILSGLLCASGVLGVAL
                     YFCRFCVTQGYFKDTVYEL"
     gene            184..1626
                     /gene="tldD"
                     /locus_tag="PKN55_02470"
     CDS             184..1626
                     /gene="tldD"
                     /locus_tag="PKN55_02470"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HNX70141.1"
                     /translation="MNSDLLLQTDALLLAPAGLDESALGGVLADMLTHQVDYADLYFQ
                     YTRGEGWSLDEGIVKSGSFHIDQGVGVRAVAGDKTAFAYSDEISLPALRRAAGAVRAI
                     AAAGGQGRAASPQVVGGRGLYLHADPIASLDAAAKVATLEKLERLARARDPRVVQVMA
                     SLAAEWDVVYIARHDGVRAADIRPLVRLSVTVIAEQHGRREQGSAGGGGRYDFSYFTD
                     AVLESYARQAVDQALVNLEARPAPAGAMTVVLGNGWPGILLHEAIGHGLEGDFNRKGS
                     SAFAGKVGQRVAAPGVTVRDDGTLPDRRGSLSIDDEGNATQCTTLIEDGILKGYMQDA
                     MNARLMGVAPTGNGRRESYAHIPMPRMTNTYMQGGAHSQEEIIASVKDGLYAVNFGGG
                     QVDITSGKFVFSASEAWKIENGKLAYPVKGATLIGNGPDVLNYVSMIGNDMKLDTGIG
                     TCGKEGQSVPVGVGQPTLRIDGGLTVGGTA"
     gene            complement(1782..2159)
                     /locus_tag="PKN55_02475"
     CDS             complement(1782..2159)
                     /locus_tag="PKN55_02475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX70142.1"
                     /translation="MLATLMRLVGVVQLLLGVLYLAVPDWLLAAMGHSSIAADIRYPL
                     GMLAARFLVYGAALLWAARQPQAHRPWIWGMVAIQLVDLVVGVSHTLAGSVPLSLSGF
                     PMFNALWISAGLAYGLRRSAAAQ"
     gene            2523..2861
                     /locus_tag="PKN55_02480"
     CDS             2523..2861
                     /locus_tag="PKN55_02480"
                     /inference="COORDINATES: protein motif:HMM:NF013777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNX70143.1"
                     /translation="MSASPCSSACPVQTVVDLIQGKWTPQILRALLPGMCRFTCLRRQ
                     LPGISAKVLSQRLRELEAQGLLLRTVLPCVPPAVEYRLSASGAELANLLGSLYHFGVR
                     HLNQHTRYAA"
     gene            2858..3319
                     /locus_tag="PKN55_02485"
     CDS             2858..3319
                     /locus_tag="PKN55_02485"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HNX70144.1"
                     /translation="MSLAPQPIARPPRLYLGTLLLSTLAHLRCPWPLWLSIWWRGALA
                     LPLLVCGGLLAWSAFATLRRAGTTGHPGRPNNELVTHGPFAWSRNPVYVAMTALYFAL
                     ALEIGSVWPLLAWLPLWLLMHYGVIRREEAYLLRRFGQAYANYLQHTRRWL"
     gene            3435..5177
                     /locus_tag="PKN55_02490"
     CDS             3435..5177
                     /locus_tag="PKN55_02490"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex family protein"
                     /protein_id="HNX70145.1"
                     /translation="MKPPRPFSACLALTLSLALLAAPALALPQLRIDAPSEVDSLLGR
                     HLALARALNDSLPPDAGQLEALRQDIDNDARSLLATEGYFSPRVRLDRQDEQWTLYVE
                     PGPRTRIGKIALQIDGPGGEGPAGAERLRALREAWSLNQDAPFRQDDWDAAKRQALRA
                     VQIDQYPTARWASTEARIDPDQQRAELLLTLASGPAFTLGPIQVHGLKRYPAEAVTRL
                     AGFTQGTPYHQRWLLDYQAALQATPYFQLVRVEAELNPEQPWLSPVHVYVQEAPKQKI
                     SVGAGYGSDSGPRGSLGYQHHNVAGLGVLFASTLQLERDQQTLDASLTLPRDGDGYRD
                     IAGVKLAHSEVQQLLLRQETVYASRLRARGDDEHGYGLTFTRERSTPDGGDTSRTQAL
                     VARYDWTRRRVDSLLNPQRGHMLAAQLVGASRYAGSDTDFLRAYARAIGFYPLDKQAR
                     VILRGEIGQVWAARANDVPIDWRFRTGGGGSVRGYAYQSLGPREAGAVTGGRVLALAS
                     AEYQHPVGPPGWRLALFADAGNASDRWQDWTPRLGLGAGLRWDSPIGPVGLDIARGQY
                     TKQWQWNLSLGASF"
     gene            complement(5279..6274)
                     /locus_tag="PKN55_02495"
     CDS             complement(5279..6274)
                     /locus_tag="PKN55_02495"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HNX70146.1"
                     /translation="MSKSLKNTLACASALAALLAGPAQAEAQLRLNLVGDVMLDDGPG
                     KVIADGRDPFAPMATLLADADYTIGNLETPVATTGRAMDSKIFTFRAHPRVLPVLKRH
                     LHAVALANNHSGDYGKTAFSETLALLKQHGIPYFGGGANLREAHTPLWLEKSGLRVAL
                     LSYNEFKPRSFEAGAQQPGIAWSEDAQVISDIRAARAAGADLVIPFMHWGWERETTPS
                     ARQRQLARLMIDHGADAVVGGHPHVTQGAETYRGKPIIYSLGNFVFDGFDRPAEKRGW
                     LLRMTLTRHGVARWDTVAADMDAEGIPHPAAQASPCGVAGNARVYACLNGARIAQ"
     gene            complement(6271..6669)
                     /locus_tag="PKN55_02500"
     CDS             complement(6271..6669)
                     /locus_tag="PKN55_02500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX70147.1"
                     /translation="MMPLRILLAACLASAALPVLATPQVACHYSYGGETHTLRAAPSA
                     SPYSVDSVPVGSYFHFRVVLRTRPADLASVEVYTYADRDEDGLALIHQASYPFPPARQ
                     PGRQYGFSGRHRVYEPVRDGELSYWCERLS"
     assembly_gap    6683..6782
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6783..7063)
                     /locus_tag="PKN55_02505"
     CDS             complement(<6783..7063)
                     /locus_tag="PKN55_02505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005654045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HNX70148.1"
                     /translation="MKKIEAVIKPFKLDEVREALSALGVNGLTVTEVKGFGRQKGHTE
                     LYRGAEYVVDFLPKMKLEVVLPDDRVEQAIEAILNSARTGKIGDGKIFV"
     gene            7190..11083
                     /gene="purL"
                     /locus_tag="PKN55_02510"
                     /gene_synonym="purI"
     CDS             7190..11083
                     /gene="purL"
                     /locus_tag="PKN55_02510"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009117159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HNX70149.1"
                     /translation="MPSILKLRGGAALSAFRSDKLHRAISEAGLPTLTIAAEYWHFAE
                     VSAQPDGAESATLARILTYGEPQLEPAPQGELFLAVPRIGTISPWSSKATDIARHCGL
                     GVVERIERGVAYWVSGAELTAEQRQQLAALLHDRMTEQVLGALDDAERLFSHVAPQPL
                     RTVDVLAGGRAALEAANREFGLALSPDEIDYLVENFTAIGRNPTDVELMMFAQANSEH
                     CRHKIFNATFVIDGKAKDKSLFGMIRDTHKAHPHGTLVAYSDNSSVIEGRTVGRFYPE
                     SSSHRYAYHEEPTHILMKVETHNHPTAISPFPGASTGSGGEIRDEGATGRGSRPKAGL
                     TGFTVSNLNIPDLIQPWEDTGCGKPSRIASALQIMLDGPIGGAAFNNEFGRPNLAGYF
                     RTFEAEVHGEMRGYHKPIMIAGGLGNIQGGQIDKHELPEGALLIQLGGPGLLIGLGGG
                     AASSMDTGSNAEDLDFDSVQRGNPEIERRCQEVIDRCWQLGEANPILSIHDVGAGGLS
                     NALPELIHGSNRGGVIDLRAVPLEESGMSPMQIWCNESQERYVLAIHPDSLDAFAALC
                     ERERCPFAVLGTATAEQQLRVRDELLGADAVDMSLEVLLGKPPRMTRDVARVKRAVTP
                     FSTEGHDLNEAVQRVLSLPAVASKSFLITIGDRTVGGMTARDQMVGPWQVPVADVAVT
                     TMGFDTLAGEAMAMGERTPVAAFDAPASGRLAVAEALLNIAAAPIGRLDQVKLSANWM
                     AAAGHPGEDAALYDTVKAVSELCQSLKVSIPVGKDSLSMKTVWEEQGEKRSVTSPLSL
                     IISAFAPVDDVTRTLTPQLRTDIAETALLLVDLGEGRSRMAGSAYAQVFQQMSGEAPD
                     LVDGKKLEAFIHALQDLNADGLVLAYHDRSDGGLFATLAEMSFASRAGMVVELDSLVK
                     AQGEHGDVLTALFNEELGAVLQIDAGQLEVVVTRLMQAGLGACLHLLGAPVAEHDALT
                     LCLGGETVFDQALTELLQTWSLTSAELQSLRDNPECAQSEFDLISEETGGLFAVVPFD
                     TEANPAAPFIQTGVRPRMAILREQGVNGQLEMAAAFDRAGFESVDVHMSDIIEGRVSL
                     NDFKGLAACGGFSYGDVLGAGEGWAKSILFNARARDQFEAFFARNDSFALGVCNGCQM
                     MSNLSEIIPGAEHWPKFRRNASEQFEARFTMVEVPDSPSILFAGMAGLQVPVVVSHGE
                     GRAVFANDDARAQALVAMRYLDHDGNATEIYPHNPNGSPDGITGLTTPDGRFTIMMPH
                     PERVFRSVQMSWRPEGLGEHSPWFRMFANARAWLA"
     gene            11208..12284
                     /gene="hemH"
                     /locus_tag="PKN55_02515"
     CDS             11208..12284
                     /gene="hemH"
                     /locus_tag="PKN55_02515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018151733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HNX70150.1"
                     /translation="MSAFLPEPSFRHDSPARLGVLLINLGTPAAPTARALRPYLKQFL
                     SDRRVVELPRWLWALILNGVILNVRPRKSAEKYASIWTPQGSPLLVNTRSQAEGLASR
                     LAGEHVVVEFAMRYGQPSVEQGVQKLRAQGVERLLLLPLYPQYAASSSGSALDAAFAA
                     LMKLRNMPEVRTVRHYHDHPAYIAALAAQVEAHWRQHGRGEKLLMSFHGVPRYTLDKG
                     DPYPCECHKTGRLLAEALGLAASDYVVCFQSRFGRTEWVKPYTSDVLRELGRQKVGKL
                     DVICPGFAADCLETLEEIAMEGKETFLTAGGGEYRYIPCLNDSTPWLDALASICREQL
                     AGWRPANADLSARKARAQAMGAKA"
     gene            12565..14220
                     /locus_tag="PKN55_02520"
     CDS             12565..14220
                     /locus_tag="PKN55_02520"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNX70151.1"
                     /translation="MRSSASWSQRLPLQTKLLLMLICINLLTVLAYTSYAIYARQQDE
                     IERLDSVLNVAAHAVPGLLAPGVLERERQPQAMSQAEYREQSQRLRRYADQVGLAYLY
                     VLTIQPDGKAAYLMDSPTAQQVAKGDAGHYMEAYPDASPMVKTAWDSGQTQSDEYTDS
                     YGAFRSVFLPMRGVQGQRYVIGADITADSVAAAMRQVLWRQLGIGAAMLLIGALLSWL
                     FARLLAASLRRITRQIGQIAESRDLSATIRVASRDELGEMAQQLNHLFEVLRQSLGKA
                     RDVAEHNAALAAQFRASAGKVQEEVAHSARQMSDMASASSQIASSANLAADIAGQVRD
                     EVRHTSQQLDAARQVLDAMMQGVQANSANSAHLADELHALNAHTGEISQVLAVIGEIS
                     DQTNLLALNAAIEAARAGEAGRGFAVVADEVRALAVRTQTTVKATQDIVTKLTSGIDR
                     AVGSMADSSRQAEQLNADSRGALSSINGMVEQIRQASSSVDQAAHQGDSIRDATGHMS
                     GQMDVVNHTLGLCADDAREIHHAATALGEQALVLQTQLASFRF"
     gene            14541..15032
                     /locus_tag="PKN55_02525"
     CDS             14541..15032
                     /locus_tag="PKN55_02525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX70152.1"
                     /translation="MLFTHLTQAMTALVDVLAQNQRRLVEAQFDVCRQWVEACRPAQL
                     NASATPLANAAEDTRLDGPMQATQSLLIAQVQAGNDLMMLSERLVSSMNRDLVKQLDK
                     SPLTGPSRDALESAVAVGGCAYESMSKATRQVNQFACTNMSAASMRAVKHARKHLSGT
                     RHH"
     gene            15292..>16658
                     /locus_tag="PKN55_02530"
     CDS             15292..>16658
                     /locus_tag="PKN55_02530"
                     /inference="COORDINATES: protein motif:HMM:NF000658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HNX70153.1"
                     /translation="MSPIETGLFSPRYAALPPIFYRVVAPQPLDAPHWAAISPDCAQL
                     LGLNPATDFNDASLAVLAGQRPPAAHPALATAYSGHQFGVWAGQLGDGRALLLGERQG
                     RDGQRYEVQIKGGGLTPFSRMGDGRAVLRSSIREFLCSEAMAGLGIPTSRALALVASR
                     TPVQRESWETAAVVTRVAPSFVRFGMFEHFCHGNRPDALRQLADHVIAAHFAHCRDAP
                     QPYLAWFTEVIAASARLAADWQAVGFCHGVLNTDNMSILGLTLDYGPFGFLDGFDPEH
                     ICNHSDHHGRYSYRRQPYIVQWNLQRLAAALWPLIREPELLAALEGYQAAYEAALAER
                     MRAKLGLLAWRDSDWGLLTDLLDVMAEARADYTLTWRRLCAVRADGGDPLGVRDLFID
                     RAAFDAWRARYAARLREDGQDDDVRASAMRAVNPAYVLRNHLAEQAIQAAQQGDYQPI
                     ERLRAA"
BASE COUNT         2924 a         5425 c         5251 g         2958 t
ORIGIN      
        1 ttggtttaag agtgactcta aagagacgct ctaaagaaat gctgaaaaaa ttgtcattcc
       61 cgcgaaggcg ggaatccaga gttttgattt tatcgggctt gttgtgcgcc agcggtgtgc
      121 tgggcgtggc cctttatttt tgccgttttt gcgtcactca aggatatttt aaggataccg
      181 tgtatgaact ctgatctcct gctccagact gacgccttgt tattggcccc tgccggcttg
      241 gatgagtccg ccttgggtgg ggtgttggcg gatatgctca cccatcaggt ggattacgcc
      301 gatttatatt ttcagtacac ccgaggtgag ggctggagcc tggacgaggg tattgtcaag
      361 tccggcagtt ttcacatcga ccagggggtg ggtgtgcggg cggtggcggg tgataaaacc
      421 gcgtttgctt attccgatga aatctcgctg ccggctttgc gccgggcggc gggggcggtg
      481 cgggcgattg cggcggctgg cggtcagggc cgggcggcga gtccgcaggt ggtgggcggg
      541 cgtggtttgt atctgcatgc cgacccgatt gccagcctgg acgccgccgc caaggtggcc
      601 accctggaaa aactcgaacg cctggcgcgc gcgcgcgatc cgcgtgtggt gcaggtgatg
      661 gccagcctgg cggcggagtg ggatgtggtg tacatcgccc gccacgacgg cgtgcgcgcg
      721 gcggatatcc gcccgctggt gcgcctgtcg gtgacggtga ttgccgagca gcacggccgc
      781 cgcgagcaag gcagcgccgg cggcggcggg cgttatgatt tcagctactt taccgacgcc
      841 gtgctggaaa gctacgcccg tcaggcggtg gatcaagccc tggtcaacct ggaagcccgc
      901 cccgcccctg ccggcgccat gaccgtggtg ctgggcaatg gctggccggg cattctgctg
      961 cacgaagcca tcggccacgg cctggaaggc gatttcaacc gcaagggcag ctcggcgttt
     1021 gccggcaaag tgggccagcg cgtggccgcc cccggcgtga ccgtgcggga cgacggcacc
     1081 ctgcccgacc ggcgcggctc gctgtcgatc gacgacgaag gcaacgccac gcaatgcacc
     1141 acgctgattg aagacggcat cctcaaaggc tatatgcagg acgccatgaa cgcccgcctg
     1201 atgggcgtgg cccccaccgg caacggccgg cgcgaaagct acgcccacat ccccatgccg
     1261 cgcatgacca acacctatat gcaaggcggc gcgcacagcc aggaagaaat catcgcctcg
     1321 gtcaaagacg gcctgtacgc cgtcaacttt ggcggcggcc aggtggacat caccagcggc
     1381 aagtttgtgt tttccgccag cgaagcctgg aaaatcgaaa acggcaaact cgcctacccg
     1441 gtgaaaggcg ccaccctgat cggcaacggc ccggacgtgc tcaactacgt gagcatgatc
     1501 ggcaacgaca tgaaactgga caccggcatc ggcacctgtg gcaaagaagg ccaaagcgtg
     1561 ccggtggggg tcggccagcc caccttgcgg attgatggtg ggttgacggt gggggggacg
     1621 gcgtaagcgg gggttttgcc agatgcggcg caatgggcca catctggcac ggttttcaca
     1681 tgatggctgc tatttgccga tcgtgatgcc gcagtacaaa ccccacgccc gccagcccga
     1741 cccgcaaccg caggccgggc cgccatccca ccgcgtggcg cttactgtgc ggcagccgag
     1801 cggcgcaagc cgtaggccag cccggcgctg atccacagtg cgttaaacat cggaaacccc
     1861 gacaagctca gcggcacgct gcctgccagg gtgtggctta ctcccaccac cagatcgacc
     1921 aactgaatgg ccaccatgcc ccagatccac ggccggtgtg cctgtggctg tcgggcagcc
     1981 catagcaggg cggcgccata caccaaaaaa cgcgcggcca gcatgcccag cggatagcgg
     2041 atatccgcgg caatgctgct gtggcccatg gcggccagca gccagtctgg cacggccaga
     2101 tacagcaccc ccagcagcag ttgcacaacg ccgaccagac gcatcaaggt tgccagcatg
     2161 agatattctc catatgtgaa tagtgttcac atatggtaag ggcagggtcg agatggcaca
     2221 agaaggcact ttttgctcag tgacttacct gtgggtaacc accaaggtgt gggcgggcgc
     2281 tgccacagcc gccaagggcg ttggcgctgt gcacaaagcg cgccgtgacc gtatttgctg
     2341 tttgcgggcg cctgtctggc cagaagcttt tgtcggggtg ttgtgcattg agccaatacc
     2401 atgacagaca agcaaaatgc gtctgagtac acaaggcggg gcttttcatg taattaaaat
     2461 taacatatgc tatgacctga tttgcccgcc aaatgccggg ccatgccatc cggagccacc
     2521 gcatgtccgc ctccccgtgt tcttctgcct gcccggttca gaccgtcgtg gacctgatcc
     2581 agggtaaatg gacaccacaa attctgcgcg ccttgctgcc cggcatgtgc cgctttacct
     2641 gcctgcgccg gcaactgccc ggcatcagcg ccaaggtgct cagccagcgc ttgcgcgagc
     2701 tggaggccca gggcctgttg ctgcgcaccg tgctgccttg cgtgccgcca gcggtggaat
     2761 accggttgag cgccagcggc gccgagctgg ccaatctgct gggcagcctg taccactttg
     2821 gtgtgcgcca tctcaatcaa cacactcggt acgccgcatg agcctcgccc cgcaaccgat
     2881 tgcccgccca ccccggcttt atctgggcac cctgctgctg tcaaccctgg cccatctgcg
     2941 ctgcccgtgg cctttgtggc tgtcgatttg gtggcgtggc gcactggcgc tgcccttgct
     3001 ggtgtgcggt ggattgctgg cctggtcggc gtttgccacc ttgcgccgcg ctggcaccac
     3061 cggccacccc ggccgcccga ataacgagct ggtgacccat ggcccgtttg cctggtcgcg
     3121 caatccggta tatgtggcga tgacggcgct gtattttgcc ctggcgctgg aaatcggcag
     3181 cgtctggccg ctgctggcct ggctgccgct gtggttattg atgcattacg gcgtgattcg
     3241 ccgcgaagaa gcatatttat tgcgccgttt tggccaagcc tatgccaatt atctgcaaca
     3301 tacccgccgc tggttataaa acccccttag cgtcgttgca cggcctggcc gaactgctgt
     3361 agccagacgg cttgtcagcc gccgtcgctg cgtatgattt tgaccccgcc gcgtgagcaa
     3421 ggtatgttaa cggcgtgaaa ccgccccgcc cattctccgc ctgcctggcg ctgacgctca
     3481 gcctggccct gctggccgca ccggccctgg ccctgccgca gttgcgcatc gacgcgcctt
     3541 ctgaagtgga cagcctgctt ggccgccatc tggcgctggc gcgcgcgctg aacgacagcc
     3601 tgccgcccga tgcaggccaa ctggaagccc tgcgtcagga tatcgacaac gatgcccgta
     3661 gcctgctggc cactgaaggg tatttttcgc cacgggtgcg actggatagg caggatgagc
     3721 agtggacgct gtacgttgag cccggcccgc gcacccgcat cggcaagatt gccttacaga
     3781 ttgatggccc cggcggcgaa ggcccggccg gcgccgagcg cctgcgcgcg ctgcgtgaag
     3841 cctggagcct gaatcaggat gcgccgtttc ggcaagacga ctgggacgcc gccaaacgcc
     3901 aggcgctgcg cgccgtgcag atcgaccaat accccactgc ccgctgggcc agcaccgaag
     3961 cgcgcatcga ccccgatcag cagcgcgccg agttgctgct gaccctggcc agtggcccgg
     4021 cgtttaccct gggcccgatt caggtgcatg gtctcaagcg ctatccggcc gaggcggtga
     4081 cgcgcttggc cggttttacc caaggcacgc cctaccacca gcgctggctg ctggactacc
     4141 aggccgccct gcaggccacg ccgtattttc agttggtgcg cgtcgaggct gaactcaacc
     4201 ccgagcaacc atggctcagc ccggtgcatg tttacgtaca ggaagccccc aagcaaaaaa
     4261 tcagcgtcgg cgccggctat ggcagcgaca gcggcccgcg cggctctttg ggctatcaac
     4321 accacaatgt ggccggcctg ggcgtgctat tcgccagcac cctgcaactg gaacgcgacc
     4381 agcaaaccct ggacgccagc ctgaccctgc cgcgcgatgg cgacggctac cgcgacattg
     4441 ccggggtcaa gctggcgcac agcgaggtgc agcaactgct gctgcgccag gaaaccgtct
     4501 acgccagccg cctgcgcgcg cgcggtgacg acgaacacgg ctacggcctg acctttaccc
     4561 gtgaacgcag cacccccgat ggcggcgaca ccagccgcac ccaggcgctg gtggctcgct
     4621 acgactggac ccgccgccgg gtggacagcc tgcttaaccc gcagcgcggg catatgctgg
     4681 ctgcgcaact ggtgggcgcc agccgctacg ccggatcaga caccgacttt ttgcgcgcct
     4741 atgcccgcgc cattggtttt tatccgctgg acaaacaagc acgagtgatt ttgcgcgggg
     4801 aaatcggcca ggtgtgggcc gcccgcgcca acgacgtgcc catcgactgg cgctttcgca
     4861 ctggtggcgg cggcagcgtg cgcggctatg cctatcaaag cctgggcccg cgcgaagctg
     4921 gcgccgtcac gggcgggcgg gtgctggcgc tggccagcgc cgaataccag cacccggtag
     4981 gcccgcccgg ctggcgactg gcgctgtttg ccgacgccgg caacgccagc gaccgctggc
     5041 aagattggac cccccgtctg ggactgggcg ccggcctgcg ctgggacagc ccgatcggcc
     5101 cggtgggtct ggacatcgcc cgtggccagt acaccaagca atggcagtgg aatttatccc
     5161 tgggggcgag cttttaagca ccactcccaa aacacagcgc tgcgcgcctg atcaggcgtg
     5221 cagccgcaga cagtacaagc ctgagcaaca acgccatgag gattttattg agcggcgctt
     5281 actgtgcgat gcgcgcgccg ttcaggcagg catacaccct ggcattgcca gccacgccgc
     5341 acggcgaggc ttgtgctgcc ggatgcggaa tgccttcggc gtccatatcc gccgccacgg
     5401 tgtcccagcg cgccacgccg tggcgggtca gcgtcatgcg cagcagccag ccgcgttttt
     5461 ctgccgggcg gtcaaagccg tcaaacacaa aattgcccag gctgtagata atcggcttac
     5521 cgcgataggt ttctgcgccc tgggtgacat gcggatggcc gcccaccacc gcgtcggcgc
     5581 cgtggtcgat catcagccgc gccaactggc gttggcgggc gctgggcgtg gtttcccgct
     5641 cccaccccca atgcatgaac ggaatcacca agtcggcccc ggcagcgcgc gcggcgcgaa
     5701 tgtcgctgat cacctgggcg tcttcgctcc aggcaatgcc cggctgctgg gcgccggcct
     5761 cgaatgagcg cggcttgaat tcgttgtagc tgagcaaggc cacgcgcagg ccggattttt
     5821 ccagccacag cggcgtgtgc gcctcgcgca gattggcgcc gccgccaaaa tacggaatgc
     5881 cgtgttgctt gagcagcgcc agcgtttcgc tgaaggcggt tttgccgtag tcgcccgagt
     5941 gattattggc cagcgccacc gcgtgcaggt ggcgcttgag caccggcagc acgcgcggat
     6001 gggcgcggaa ggtgaagatt ttgctgtcca tcgcccggcc ggtggtggcg accggggttt
     6061 ccagattgcc gatggtgtag tccgcatcgg ccagcagggt agccattggc gcaaatgggt
     6121 cgcggccgtc ggcaatcacc ttgccggggc cgtcgtccag catcacatct cccaccagat
     6181 tcagccgcaa ctgcgcctcg gcctgcgccg ggccggccag cagggcggcc aaggcgctgg
     6241 cgcaggccag cgtgtttttc aatgatttgc tcatgacagg cgctcgcacc agtagctcaa
     6301 ctcgccgtcg cgcaccggct cgtacacccg gtggcggccg ctgaagccat attgccggcc
     6361 aggctggcgg gccggcggaa agggatagct ggcttggtga atcaaggcca ggccgtcttc
     6421 gtcgcggtcg gcgtaggtgt acacctcgac actggccaaa tccgccggcc gggtgcgcaa
     6481 caccacgcga aaatgaaaat acgagcccac cggcacgctg tccacgctgt agggcgaggc
     6541 gctgggcgca gcgcgcaggg tgtgggtttc gccgccatag ctatagtggc aggccacttg
     6601 cggcgtggcc agcacgggca gggcagcgct ggccagacag gcggccagca aaatacgcag
     6661 aggcatcatg gcaactcgcc aannnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6781 nnaacacaaa aatcttgccg tcgccaattt tgccggtgcg tgcgctgttg aggatggctt
     6841 cgatggcctg ctcgacgcga tcatccggca gcacgacttc cagcttcatt ttgggcagaa
     6901 aatccaccac atattcagcg ccgcgataca gctcggtgtg gcctttctgg cggccaaaac
     6961 cctttacttc agtgacggtc aggccattca cccccagcgc cgacagcgcc tcgcgcactt
     7021 cgtccagttt gaacggcttg atgacggctt cgatcttttt catgctgact cctgtaataa
     7081 ttcaattgaa tccgtggttt agcaaatttt cgcgtcgcaa tcccgctcga aacggacggc
     7141 gttttgatgt attatgaccg ttttttaccc gttgctgcga gttccctcaa tgccttccat
     7201 tctcaagctt cgcggcggcg ccgcgctgtc tgcgtttcgt tccgacaaac tgcatcgtgc
     7261 catctctgaa gccggattgc caaccctgac aattgccgca gaatactggc acttcgccga
     7321 ggtgtcggca cagccggatg gggcggaatc cgccaccctg gcgcgcattc tgacgtatgg
     7381 tgagccgcag ctcgaacccg ccccgcaagg cgagctgttt ctggccgtgc cgcgcatcgg
     7441 cactatctcg ccgtggtcgt ccaaggccac cgacatcgcc cgccattgtg gcctgggcgt
     7501 ggtcgagcgc atcgagcgcg gcgtggccta ctgggtcagc ggcgccgagc tcactgccga
     7561 gcagcgccag cagctggccg cgctgctgca cgaccgcatg accgaacaag tgctgggcgc
     7621 gctggacgac gccgagcggc tgttctcgca tgtcgcgccg cagcccttgc gcaccgtgga
     7681 cgtgctggct ggcggccgcg ccgcgctgga agccgccaac cgcgaattcg gcctggcgct
     7741 gtcgccggac gaaattgatt atctggtgga aaacttcacc gccatcggcc gcaacccgac
     7801 cgacgtcgag ctgatgatgt tcgctcaggc caactcggaa cactgccgcc acaagatttt
     7861 caacgccacc tttgtgatcg acggcaaggc caaggacaaa agcctgttcg gcatgatccg
     7921 cgacacccac aaggcgcatc cgcacggcac gctggtggcg tattcggata actcgtcggt
     7981 gatcgaaggc cgcactgtcg gccgcttcta cccggaaagc agcagccatc gctacgccta
     8041 ccacgaagag ccgacccata tcctgatgaa ggtggaaacc cacaaccacc cgaccgcgat
     8101 ttcgcccttc cccggcgcat ccaccggctc gggcggcgaa attcgcgacg agggcgccac
     8161 cggccgcggc tcgcgcccca aggcgggcct gaccggcttt accgtctcca acctgaatat
     8221 cccggatctt atccagccgt gggaagacac cggctgcggc aagccctcgc gcattgcctc
     8281 ggccctgcaa atcatgctgg atggcccgat tggcggcgcg gcgttcaata acgaattcgg
     8341 ccgccccaat ctggctggct acttccgcac ctttgaagcc gaagtgcacg gcgagatgcg
     8401 cggctatcac aagccgatca tgatcgctgg cggcctgggc aatatccagg gcggccagat
     8461 cgacaaacac gaactgccgg aaggcgcgct gctgatccag ctgggcggcc ccggcctgtt
     8521 gatcggcctg ggcggcggcg ctgcgtcgtc gatggacacg ggttctaatg ccgaagacct
     8581 ggatttcgac tcggtgcagc gtggcaaccc ggaaatcgaa cgccgctgcc aggaagtcat
     8641 cgaccgctgc tggcagctgg gcgaagccaa cccgattctg tcgattcacg acgttggcgc
     8701 tggcggcctg tccaatgccc tgcccgagct gattcacggc tccaatcgtg gcggcgtgat
     8761 tgatctgcgc gccgtgccgc tggaagaatc cggcatgtcg ccgatgcaaa tctggtgcaa
     8821 tgaatcgcaa gagcgctatg tgctggccat tcatccggac agtctggacg cctttgccgc
     8881 gctgtgcgag cgcgagcgct gcccgtttgc tgtactgggt accgccaccg ccgaacaaca
     8941 gctgcgcgtg cgcgacgagc tgctgggcgc cgacgccgtg gacatgtcgc tggaagtgct
     9001 gctgggcaaa ccgccgcgca tgacccgcga cgtggcccgc gtcaaacgcg ccgtcacccc
     9061 gttctccacc gaaggtcatg atctgaacga ggcggtacag cgcgtgctca gcctgccggc
     9121 cgtggccagc aagagcttct tgatcaccat cggcgaccgc accgtgggcg gcatgaccgc
     9181 gcgcgaccag atggtcggcc cgtggcaggt gccggtggcc gatgtggcag tcaccaccat
     9241 gggctttgac accctggccg gcgaagccat ggccatgggc gagcgcaccc cggtggcggc
     9301 cttcgacgcc ccggcctctg gccgcctggc ggtggccgaa gcgctgctga acattgccgc
     9361 tgcgccgatt ggccggctgg atcaggtcaa gctgtcggcc aactggatgg ccgccgctgg
     9421 ccaccctggc gaagacgcgg ccttgtacga cacggtgaaa gctgtgtccg agctgtgcca
     9481 gtcgctgaaa gtgtcgatcc cggtgggcaa ggattcgctg tcgatgaaga cagtgtggga
     9541 agagcaaggc gaaaaacgca gcgtcacctc gccgctgtcg ctgatcatct cggcgtttgc
     9601 cccggtggac gacgtcaccc gcacgctcac cccgcaactg cgcaccgaca ttgccgaaac
     9661 cgccttgctg ctggtggatc tgggcgaagg ccgcagccgc atggccggct cggcctatgc
     9721 gcaggtgttc cagcaaatga gcggcgaagc gccggatctg gtggacggca aaaagctcga
     9781 agcgtttatc catgcgctgc aagacttgaa cgccgacggt ctggtgctgg cctatcacga
     9841 ccgctcggac ggcggcctgt tcgccacgct ggcggaaatg agctttgcca gccgcgccgg
     9901 catggtggtc gagctggata gcctggtgaa agcccagggc gagcacggcg atgtgctgac
     9961 cgccttgttt aacgaagaac tgggcgccgt gctgcaaatc gacgccgggc aactggaggt
    10021 cgtggtcacc cgcctgatgc aggccggcct gggtgcttgc ctgcacctgc tgggcgcgcc
    10081 ggtggccgag catgatgcgc tgacgctgtg cctgggcggc gagacggtgt ttgatcaagc
    10141 gctgaccgag ctgctgcaaa cctggagcct gaccagcgcc gagctgcaat cgctgcgcga
    10201 caaccccgag tgcgcgcaaa gcgaattcga cctgatcagc gaagaaaccg gcgggctgtt
    10261 tgccgtcgtg ccgtttgaca ccgaagccaa cccggcggcg ccgttcatcc agaccggcgt
    10321 gcgtccgcgc atggccatcc tgcgcgaaca gggcgtcaac ggccagttgg aaatggccgc
    10381 ggcgtttgac cgcgctggct ttgaatcggt ggatgtgcac atgagcgaca tcatcgaagg
    10441 ccgtgtgtcg ctgaacgact tcaagggctt ggccgcttgc ggcggcttca gctacggtga
    10501 cgtgctgggc gctggcgaag gctgggccaa gagcattctg ttcaatgccc gcgcccgcga
    10561 ccagttcgag gccttctttg cccgcaacga ttcgtttgcc ctgggcgtgt gcaatggctg
    10621 ccagatgatg tccaacctgt cggaaatcat ccccggcgcc gagcactggc cgaagttccg
    10681 ccgcaatgcc tccgagcagt tcgaagcgcg cttcaccatg gtggaagtgc cggacagccc
    10741 atccatcctg tttgccggca tggcgggctt gcaggtgccg gtggtggtca gccacggcga
    10801 gggccgtgcg gtattcgcca acgatgacgc ccgcgcccag gcattggtgg ccatgcgtta
    10861 tctggatcat gacggcaacg cgaccgaaat ctacccgcac aacccgaacg gttcgccgga
    10921 cggcatcacc ggtttgacca cgccggatgg ccgcttcacc atcatgatgc cgcaccccga
    10981 gcgggtgttc cgcagcgtgc agatgagctg gcgtccggaa ggcctgggcg agcacagccc
    11041 gtggttccgc atgttcgcca acgcccgcgc ctggctggct tgatccacct gtgacgttcc
    11101 cgcgccggag gctggcctct ggcgctgtgc ggcccgcccg gaacctccgg cgcgggccgt
    11161 gtttccaatg tgcccggctt tttgctgaat gatgaggctg aatcctgatg tctgcttttc
    11221 tgcctgaacc cagttttcga cacgacagcc cggcccgcct gggcgtgctg ctgatcaacc
    11281 tgggcacccc ggccgcgccc accgcgcgcg cgctgcgccc ctacctgaag caatttttgt
    11341 ctgaccgccg cgtggtcgag ctgccgcgct ggctgtgggc gctgattctg aacggcgtga
    11401 ttctgaatgt ccgcccgcgc aaatccgccg agaaatacgc ctcgatctgg acgccgcaag
    11461 gctcgccgct gctggtcaac acccgcagcc aggcggaagg cctggccagc cggctggccg
    11521 gtgaacacgt ggtggtggag ttcgccatgc gctacggcca gccctcggtc gagcaaggcg
    11581 tgcaaaagct gcgcgcccaa ggcgtggagc gcctgttgct gctgccgctg tatccgcaat
    11641 acgccgccag cagcagcggc agcgcgctgg acgccgcctt tgccgcgctg atgaaactgc
    11701 gcaatatgcc ggaagtgcgc accgtgcgtc attaccacga ccacccggcc tatatcgccg
    11761 cgctggccgc ccaggtggag gcgcattggc gtcagcacgg ccggggcgaa aaactgctga
    11821 tgagctttca tggcgtgccg cgctacaccc tggacaaggg cgacccctac ccgtgcgaat
    11881 gccacaaaac cggccgcctg ctggccgaag cgctgggtct ggctgccagc gactatgtgg
    11941 tgtgttttca gtcgcgcttt ggccgcaccg agtgggtaaa gccctacacc agcgatgtgc
    12001 tgcgcgaact gggtcggcag aaggtgggca agctggacgt gatctgcccc ggctttgccg
    12061 ccgattgcct ggaaaccctg gaagaaatcg ccatggaagg caaggaaacc tttctgaccg
    12121 ctggcggcgg cgaataccgc tacatcccct gcctgaacga cagcacaccg tggctggatg
    12181 cgctggccag catctgtcgc gaacaactgg ccggctggcg ccccgccaac gccgacctga
    12241 gcgcccgcaa ggcccgtgcc caggcaatgg gcgccaaggc ctgagtctgc ttgtccgagc
    12301 aatcccctgt cgttcgcgcc gcttacaaaa cccagcacag cgtcctggcc agacgctcga
    12361 ccgccatgcg gaacaacgcg agggtttgtg agcggctctt aggcagagtg ttgcgtggca
    12421 tgtgcggcaa gctgcgcagc accgggcaag atacgtgacc tgatcggcat attgccgtaa
    12481 cattaccgct gcatgaccgg gcgtgcccaa cgttcgtgct gtgtatggtt tctccccccg
    12541 ctcgtcccgc aacaggattt caccatgcgt tcttctgcca gctggtcaca gcgcctgccg
    12601 ctgcaaacca aacttttgtt gatgttgatt tgcattaacc tgctgaccgt gctggcctat
    12661 accagttatg ccatctacgc gcgccagcaa gacgaaatcg aacggctgga cagcgtgctg
    12721 aatgtggccg cccatgccgt acctggcctg ctggcccccg gcgtactgga gcgtgagcgc
    12781 cagccgcagg ccatgagcca ggccgaatac cgcgaacaaa gccagcgcct gcgtcgttat
    12841 gcagatcaag tgggtctggc ttatctttac gtgctcacca tccagcccga cggcaaggcc
    12901 gcctacctga tggacagccc cactgcccaa caagtggcca agggtgacgc cggtcactat
    12961 atggaagcct atcccgacgc cagcccgatg gtaaaaaccg cctgggacag cggccagact
    13021 caaagcgacg aatataccga ttcttacggc gccttccgct cggtgtttct gcccatgcgc
    13081 ggtgtccaag gccagcgcta tgtgattggc gccgacatca ccgccgattc ggtggccgcc
    13141 gccatgcggc aagtcttgtg gcgccaactg ggcattggcg cggccatgtt gctgattggt
    13201 gcgctgctct cctggctgtt tgcccgcttg ctggcagcca gcctgcgccg catcacccgc
    13261 cagattggcc agattgccga aagccgcgac ctgtctgcca ccattcgcgt ggccagtcgc
    13321 gatgaattgg gcgagatggc ccagcaactg aatcacttgt ttgaggtact gcgtcaatcg
    13381 ctgggcaagg cacgcgacgt ggccgagcac aatgccgccc tggccgcaca atttcgcgcc
    13441 agcgccggca aagtgcagga agaagtcgcc catagcgccc ggcaaatgag cgacatggcc
    13501 agcgcctcca gtcagattgc cagctcggcc aatctggccg ccgacatcgc cggccaggtg
    13561 cgcgacgaag tgcgccacac cagccagcag ctggatgccg cccgccaggt gttggacgcc
    13621 atgatgcagg gggttcaggc caactcggcc aacagcgccc atctggccga cgagctgcac
    13681 gcactgaacg cccacaccgg cgaaatcagc caggtgctgg cagtgattgg tgaaatttcc
    13741 gaccagacca atctgctggc gctcaacgcc gccatcgaag ccgcccgtgc aggcgaagcc
    13801 ggacgtggct ttgccgtggt ggccgacgaa gtgcgcgcct tggcggtgcg cacacaaacc
    13861 acggtgaaag ccacccagga catcgtgacc aagctcacca gtggcatcga ccgcgccgtg
    13921 ggcagcatgg ccgatagcag ccgccaggcc gaacagctca acgccgactc acgcggcgcg
    13981 ctgtccagca tcaacggcat ggtcgagcaa atccgccagg cctcgtccag tgtggatcaa
    14041 gctgcccacc agggcgacag catccgcgac gccaccgggc atatgtccgg ccagatggac
    14101 gttgtcaacc acacgctggg cttatgtgcc gacgacgccc gcgaaattca ccacgccgcc
    14161 accgccctgg gcgaacaggc gctggtgctg caaacacagt tggccagctt tcggttttga
    14221 gggttttttg ccgttggcat ctgtctgatg gagggtgtgt cgcgttcctg caacgccgtc
    14281 ccaagcgggc ggcgcccatt gctgacaatg gcaaatccag tcctgacaag caactgacta
    14341 gcgggtaagt aaaaacacct gtagtttatg caataggtat attgacccca tggtaaaacg
    14401 gggtaaactg gctgtaatgt gacgcagtgc agactgtttg ccagccaagg caaaaagcgc
    14461 acggcgtttt ttctggtcaa gtcaacccgg gcgcgccgct tcactgagca ccggtcccgc
    14521 catcggcacg gagaacccac atgctgttca cccatctgac gcaagccatg acggcgctgg
    14581 tcgacgttct cgcgcaaaac cagcgccgac tggtcgaagc ccagtttgat gtctgtcggc
    14641 aatgggtgga agcctgccgt ccggcgcagc tcaatgccag cgccacgccg ctggccaatg
    14701 ccgccgaaga cacccggctg gatggcccga tgcaggccac ccaatccttg ctgattgccc
    14761 aggtgcaggc cggcaacgac ctgatgatgc tgtccgaacg cctggtgtcc agcatgaacc
    14821 gcgatctggt caagcagctg gataaaagcc cgctcaccgg ccccagccgc gacgcgctgg
    14881 aatccgccgt cgccgtcggt ggctgtgctt atgaatccat gtccaaggcc acccgtcagg
    14941 ttaatcagtt tgcctgtacc aatatgtctg ctgcctcgat gcgcgcggtc aagcatgcac
    15001 gcaaacacct gagcggcacc cggcatcact gacccggcgg ttaagctttc tgccgtggtt
    15061 gtgtggcctt gccgcactac gccgcgcggc tggcccggcg tgtgcggcgt tgagtgtgat
    15121 gagtagcgcc aggccgtgca cacggtactc acttggccat ctggctgccg tcgtctgccc
    15181 ggcgggcggc cagaatagcc cgccagacca cgccatcccc tcaccacaga agggttttgt
    15241 gcgcggcgct tactgctata gtaagctatc cccccactcg cacgacctgc catgtccccg
    15301 atcgaaaccg ggctgttcag cccccgttac gccgccctgc cgccgatttt ttaccgtgtg
    15361 gttgccccgc aaccgctgga cgccccgcac tgggcggcca tcagcccgga ttgcgcccaa
    15421 ctgctgggct tgaaccccgc cactgacttt aacgacgcca gtctggccgt gctggccggc
    15481 cagcgcccgc ccgccgcgca tccagcgctg gccaccgcgt attccggcca ccagtttggc
    15541 gtgtgggccg gccagttggg cgatggccgc gcgctgctgc tgggcgagcg ccagggccgc
    15601 gatggccagc ggtatgaagt gcaaatcaag ggcggcgggc tgacgccgtt ttcgcgcatg
    15661 ggcgacggcc gcgccgtgct gcgctcgtcg attcgtgaat ttttgtgttc agaagccatg
    15721 gccgggctgg gcattcccac cagccgggcg ctggcgctgg tggcctcgcg cacgccggtg
    15781 cagcgcgaaa gctgggaaac cgccgccgtg gtcacccgcg tggcgcccag ctttgtgcgc
    15841 tttggcatgt ttgagcattt ttgccacggc aaccgcccgg acgcgctgcg ccagctggcc
    15901 gaccatgtga ttgccgcgca ttttgcccac tgccgcgacg cgccccagcc ctatctggcc
    15961 tggtttaccg aagtgattgc cgccagcgcc cggctggcgg ctgactggca ggcggtgggc
    16021 ttttgccacg gcgtgctcaa caccgacaat atgtccatcc ttggcctgac gctggactac
    16081 ggcccgtttg gctttctgga tggttttgac ccggaacaca tctgcaacca ctctgaccac
    16141 cacggccgct acagctaccg ccgccagcct tatatcgtgc aatggaatct gcaaaggctg
    16201 gccgcggcgc tgtggccgct gattcgcgag cccgagctgc tggcggcgct ggaaggctat
    16261 caggcagcgt acgaagccgc gctggccgag cgcatgcgcg ccaagctggg cctgctggcc
    16321 tggcgcgaca gcgactgggg cttattgacc gatttgctgg atgtgatggc cgaggcgcgt
    16381 gcggattaca cgctgacctg gcggcgcttg tgcgcggtgc gcgccgatgg cggcgacccg
    16441 ctgggcgtgc gcgatttgtt tatcgaccgc gccgccttcg acgcctggcg cgcgcgctac
    16501 gccgcgcggc tgcgcgagga cggtcaggac gacgacgtcc gcgccagcgc catgcgcgcg
    16561 gtgaatccgg cctatgtgct gcgcaatcac ctggccgagc aggccattca ggccgcgcag
    16621 cagggcgatt atcaaccgat tgagcggctg cgcgccgc
//
LOCUS       DAOPYX010000026        16041 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_3883_length_16041_cov_8.463218, whole genome shotgun sequence.
ACCESSION   DAOPYX010000026 DAOPYX010000000
VERSION     DAOPYX010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 16041)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16041)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16041
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3883_length_16041_cov_8.463218"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..750
                     /locus_tag="PKN55_02535"
     CDS             <1..750
                     /locus_tag="PKN55_02535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003822006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HNX70154.1"
                     /translation="IETQFHHLVRTVPGKGRLVVNGREASLDRVLARGCWTPVERFGE
                     TGEWSCGPVDADGACDILLVGMPQGRLNWRLLGEHNRLNALAAIAAARHVGVPPAVAI
                     DALSRFANVKRRMEVKGVAGGVTVYDDFAHHPTAIATTLAGLRAKVGAARILAVLEPR
                     SNTMKLGTMKAELPGSLADADQVFCYGANLGWDAAEALAPLGAQAQTFDQLDALVAAV
                     RQAARAGDHVLVMSNGGFGGVHGKLLAALAD"
     gene            750..1682
                     /locus_tag="PKN55_02540"
     CDS             750..1682
                     /locus_tag="PKN55_02540"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNX70155.1"
                     /translation="MEHAENAMPAALSDASLPLVSIGIVTYNQKDFLRECIESALAQD
                     YPHLEIVVADDGSKDGTHEMLRDYQARYPGKFVLRLAERNQGITANSNAAHFACTGKY
                     IAWMGGDDLLLPGKIRRQVEFMEAHPDCTLCYHNLDVFQSDTDQTLFLFNARFHPEGD
                     VRTAIRHGTFNGACSTMVRRDKTPARGFNPAIPMASDWLYWVETLHHGGSVRYLDEVL
                     GRYRRHGGNVTASGQHRLAQTHVDHLSSCQYILALDIDYLDDVMHAYSQKLLALRQHA
                     HFAKFAWYSFVLSPSLKGAVRLLLYYISLGKIRK"
     assembly_gap    1687..1786
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1787..2955)
                     /locus_tag="PKN55_02545"
     CDS             complement(<1787..2955)
                     /locus_tag="PKN55_02545"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNX70156.1"
                     /translation="MNTLSAPLDGRRDLEVISLIGLVHGVSHFFHLLLPPLFPWLMAE
                     FGLSFAAIGVTMTVFFVVSGVGQAMAGFLVDRFGAARVLAGGISCFVLAGLTLHFATG
                     LPMLVAVAAMAGLGNSVFHPADFTVLNRHVSPARLGHAFSAHGLSANLGWACAPLLLT
                     TLASAYGWRQAALGAVLVALLGLLALAWRHAAIADPRHGASRATEARASTLDFLRSRP
                     VWLCFLFFLFTAAAFGAIQNFVSPILHAVYGLARPTAALALSVYMLGSAAGVILGGFL
                     AQKNAHDHLIAAALGLAAVLAALLAGQWLPGWSMLPLMAGMGFFTGVAGPSRDLLVRR
                     AATARFGQSAFGRIYGFVYSGLDLGLACAPLLFAGWMDAGQFSAVLVGIAVLQALA"
     gene            3046..3840
                     /locus_tag="PKN55_02550"
     CDS             3046..3840
                     /locus_tag="PKN55_02550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012431326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNX70157.1"
                     /translation="MQTYPECDPLPRRPPTADEPVIVNLRHLPPDVRVPAHRHDWAQL
                     AYPLRGAMRLSAAGMVWLAPALRAVWIPPQIEHEVVVLGEVELRTVYIAPDVAPLSLS
                     SCAVIEVSDLMRALFEALSDTQPDSADAPLRRQQLTALLLTEIRRAPPLSLGLPMPQD
                     RRLHALCQALIAQPASPLTLAEWAARVGASERTLARGFQRELRMSFAAWRQQLRLAHA
                     LDGISRGRPLGEVASELGYASQPAFSAMFKRAFGVPPGQFLRQHRA"
     gene            complement(3859..4611)
                     /locus_tag="PKN55_02555"
     CDS             complement(3859..4611)
                     /locus_tag="PKN55_02555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX70158.1"
                     /translation="MSHAAPLPDLPQYQSPMFIPRYQPFSGARWSLRHADMVLCPGYW
                     TPPQLVNGMALLTRIDESGKPHTWMSMSPMEIESQEIGCQHARGHVVVMGLGMGWAAA
                     NAALHPAVSQVTVVEFDPEVIAAVSQSGIFDQLPESARARVRVVQGDAYQYVPDTPAD
                     TLLADIWLPLFGAERDAEVRRMCANTGAGRVYFWGQEMVIAHRARQRGLAINADTVAE
                     LVAEMALPLIGPAECPDYPQRIELAAARWLKA"
     gene            complement(4761..4845)
                     /locus_tag="PKN55_02560"
     tRNA            complement(4761..4845)
                     /locus_tag="PKN55_02560"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4809..4811),aa:Leu,seq:taa)
     gene            complement(4911..6482)
                     /locus_tag="PKN55_02565"
     CDS             complement(4911..6482)
                     /locus_tag="PKN55_02565"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNX70159.1"
                     /translation="MKVSTRLFAQALLAALTLLAVMAACAWQLADIRHTLERAQSSQA
                     AVHQLTAAKAAALELAKADPVLPDTAALLTRTSAEVTRALNSVARAEPAASKLLTPWR
                     EYVRQFDSAVRIAASSPADALAIPEQIYAMHLQPMLRQLDALSNALSAQARADAEAAD
                     AGLRQLLWSVLAPLGVSALLIVGSQTWLAGRLSRRLAGLARDARALAAGDLAVRLPNQ
                     GRDEIDQLAAAFNHFVDDLATLLTRAQHEAQVIDDNARSLAGMGASVSAQTRDQSEAV
                     HAASAQIEAISQDVHHIALAASQASDEAADAELLASQASEMGGRAMRALDESQQAVAG
                     AGGHFAVFAQRLAGIEQLSQAIGEIAEQTNLLALNAAIEAARAGESGRSFAVVADEVR
                     KLAERTRQSTQDITRLASEVHDDLGAARTAMTVSRDAVEGAAAEGQAMADALVCITRG
                     VRQVAGHIHDMAERTETQSQACHALAGHLERVASGACQAAAEVDATHQRIEALASIAQ
                     AKQQNMARYVGVVTS"
     gene            complement(6733..7248)
                     /locus_tag="PKN55_02570"
     CDS             complement(6733..7248)
                     /locus_tag="PKN55_02570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018150382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HNX70160.1"
                     /translation="MKQPALIVIDVQNDYFADGAFPLWQADAALSQVEAAVAHARAHH
                     VPVILVQHIAAGPAPFFNRDSHGVAIHPRLLAAAPEAPVVIKTHADSFLETDLQATLD
                     ALGVDSLWLCGMMTHNCVTHTALSPAAARYPVTVLGDASTTVSEILHILALRALAPRV
                     AVKTVAALSAS"
     gene            7376..8329
                     /locus_tag="PKN55_02575"
     CDS             7376..8329
                     /locus_tag="PKN55_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018150381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNX70161.1"
                     /translation="MPRVHPVAILAFDRISAFHLAAPCVVFGEACPLPECFPVQVCAW
                     EAGPLRTTSGFTLSVEHGLEALARAEVVIVPSWRDPAERAPEAVLDALRAAHARGAQV
                     VGLCLGAYLLAQAGLLDGRGATTHWAYAADFARRHPQVRLNADVLYLADGALLTSAGT
                     AAALDCCLQLLRERLGAEDANRAARRLVIAPHRAGGQAQFIERPLPSTRDDARLAGLL
                     DEVRAQLDAPHSLDALAARALMSRRTFTRRFRQLTGQSFQAWLLGERLAFSQRLLEVS
                     ALPIERIAALAGFGSPEAFRLHFRRSFAVSPSAWRQAFTGG"
     gene            complement(8336..12673)
                     /locus_tag="PKN55_02580"
     CDS             complement(8336..12673)
                     /locus_tag="PKN55_02580"
                     /inference="COORDINATES: protein motif:HMM:NF024376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNX70162.1"
                     /translation="MIRMVLSLWLCTLCWLATAAEPVRIGVLAYRPKAQTLAQWQPLA
                     QALKQAIPERDFRVEAYSFPELEAAVARKQVDFVLTNPGHFVLISHQQKLSAPLATLV
                     NDVNGTPSASFGGVVFSLRGRGLDTPERLHGKTVAFTSTMSLGGYQMQALALREAGLD
                     LKRDTLWLTTGAPHDKVVQAVLSGRADAGLVRTGLLEELAGEGRLRLEDIEVMHLPDT
                     PDGQLPWLTSTELYPEWPFVALAGVDAALAKRVAVALLQLPHDAPLTRAMGIHGFTIP
                     ADYGSIEHLLLTLRMPPFDRGPAFTLSDIWARYALSVVLGALGLLVIAGLLFRLWLTN
                     RSLAIERQRVLMHSQALRDSEFRWQCALEGTGDGMWDWDLQDNRVEYSRGWLDMLGYA
                     PGELPAHYQAWLDLLHPQDRTAALAGLAAYLDGHHGHYQLEFRLRSKQGDYKWILSRG
                     MVVSRGPDDQPLRMIGTHTDISERHLAERHEQFRSRVLDLLARGEPLPALLMTVVHEV
                     EQMHPAMLCSILLLNSQTGCLGQGIAPSLPAFYNAAVEGLKIGPDVGSCGAAAHRGQR
                     VVVADIAHHPNWAPYAELACQAGLAACWSQPILSADGQVLGTFAIYHRQPHTPTAADI
                     VLIEQCARLISLAIERRQAEAQLAESETRYRSLIELAHEGIVVVQDGRICYCNPTMEH
                     MANAGPAGLYGQGFLALVHADDQRQVARQLHPLPGSSGHGVPRCQFRLGNGESPRWIE
                     MSSTPINWQGRPAMLNSLSDISERRRTEQQLQLAASVFVHAREGIMITDAEGNIIDVN
                     DTFTRITGYSRDEALGQNPRMLRSGRHDREFYAAMWRSLLDKGHWYGEIWNQRKNGEI
                     FAEMQTISAVCDEDGHTRHYVALFSDITLIKAHEQQLEHIAHYDALTGLPNRVLLADR
                     LQQAMSQTQRRSQRLAVVYLDLDGFKAINDRHGHDIGDQLLMQLARRMRDTLRDSDTL
                     ARLGGDEFVAVLMDLDDTQASAPALTRLLAAAAQPVMLGNQPLQVSASLGVTFYPQEG
                     EVDADQLMRQADQAMYQAKLAGKNRYHLFDAAHDHHIKGQHESLDRIRRALQAGEFVL
                     HYQPKVNMRRGEVIGAEALLRWQHPDKGLLPPAAFLPLVDAHPLAVDLGEWVIDQALT
                     QMSLWQAQGLILPVSVNIGARQLQQARFVSRLREILDSHAEVDPEHLELEILETSALE
                     DLAEVAQVIAACRAMGVKFALDDFGTGYSSLTYLKHLQVARLKIDQSFVRDMLDDPDD
                     LAILSGVIGLAAAFRRDVIAEGVETAEHGAMLLQLGCEEAQGYGIARPMPASKLPAWA
                     AHWQGDPNWRNLPALNRDDLPLLFARTELRAWMTAVTLYLRGERASAPTLDEAHSRFA
                     HWLANEGARRHGAEPLFIHLCEVHAELHAFGRQLCQASAQPAPGELGQLHQRGEVVLA
                     TLRQLARNV"
     gene            complement(12770..14392)
                     /locus_tag="PKN55_02585"
     CDS             complement(12770..14392)
                     /locus_tag="PKN55_02585"
                     /inference="COORDINATES: protein motif:HMM:NF024140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNX70163.1"
                     /translation="MLRQLTIGPRLGAGFAVLVMLMALIAGAATLAINAMQAGARQIV
                     GRDLVKTELAHTVMETAAANAMKLVRLVAAPSQEARIPLYKDIDANKAVLDKALAQLL
                     PLADTPQETTLLEQVKAQGQAYNQIFVDAADLLEGDERAEAQKLLETRTLPALDSLVA
                     TSRQVVELETHRAEQGAQDADARSQQAKQAMLALGGASLLISVLLAWRITRSIVRPLH
                     EAGAAVERVAAGDLRTPLQAEGRDEPALLLRGVARMQGSLREMIGAVNAGAAQVGESA
                     DTLARAAEQIAQRSQAQDRMASTIATAVEALNQQAHSVADTAQHTRSLAEEAVALAEH
                     GRGLVGDAARDVGRLSDAVAASAREVNAVQQRSDSIAGSVDTIRELSDQTNLLALNAA
                     IEAARAGEQGRGFAVVADEVRALATRATQSTHEIHQMIDAMRQQTRAAVQGMDAGVGE
                     VRQGVAQVESIVEPLATLSQRSRASFESMEQLAHAAEQQSGAAQDILRHSRDIAVLAS
                     ENHGLVRDAVSTSQALTEAASRMRQAMAQFSC"
     gene            complement(14643..15545)
                     /gene="cysM"
                     /locus_tag="PKN55_02590"
     CDS             complement(14643..15545)
                     /gene="cysM"
                     /locus_tag="PKN55_02590"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014088132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase CysM"
                     /protein_id="HNX70164.1"
                     /translation="MSIPPTFHTLEDFVGHTPLVRLKRMARGRNNVVLVKLEGNNPGG
                     SVKDRPALSMIRRAEARGDIRPGDTLIEPTSGNTGIGLAMAAAMLGYRMVLVMPENSS
                     AERVATMKAYGAEVVLTPASASMPGAIDEARRRIEAGEGRFLDQFGNPDNPQAHFDST
                     GPEIWTDTAGTITHFVSSMGTTGTIMGTSRYLKSQNPAIQIVGVQPEPGSQIPGIRKW
                     EPEYLPKIYDFSRIDRLELVSQAEAEDTVRRLAREEGILAGPSSGGALAVALKLSEQL
                     ENAVIVSIVCDRGDRYLSTGLFPD"
     gene            complement(15653..>16041)
                     /locus_tag="PKN55_02595"
     CDS             complement(15653..>16041)
                     /locus_tag="PKN55_02595"
                     /inference="COORDINATES: protein motif:HMM:NF024677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TRAFs-binding domain-containing protein"
                     /protein_id="HNX70165.1"
                     /translation="LAIAEYLRGFEADWRDAYPGVNAVTLMEMQETPDPRQAELLPVV
                     RYAVARRMASGQPDYWDYATQLELAVLARDLIGAQTALADALARRREPWEGETTAKNL
                     QFIIDARHPRGEDCGWMHALVAALRA"
BASE COUNT         2861 a         5326 c         5081 g         2673 t
ORIGIN      
        1 attgaaaccc agtttcacca tctggtgcgc accgtgcccg gcaagggccg gctggtggtc
       61 aacggccgcg aagccagcct ggaccgcgtg ctggcgcgcg gctgctggac gccggtggag
      121 cgctttggtg agaccggcga atggagctgc ggcccggtgg acgccgacgg cgcctgcgac
      181 atcctgctgg tgggcatgcc acaaggtcgg ctgaactggc gcctgctggg cgaacacaac
      241 cgcctgaacg cactggccgc cattgccgcc gcccgccacg ttggcgtgcc gccagcggtg
      301 gcgattgatg cgctcagccg ctttgccaac gtcaagcgcc gcatggaagt caaaggcgtg
      361 gctggcgggg tgacggtgta cgacgatttc gcccaccacc ccacggcgat cgccaccacg
      421 ctggccggcc tgcgcgccaa ggtgggcgcg gcgcgcattc tggccgtgct ggagccgcgc
      481 tccaacacca tgaagctggg cacgatgaag gccgagctgc ccggcagcct ggccgacgcc
      541 gatcaggtgt tttgctacgg cgccaatctg gggtgggacg ccgccgaggc gctggcccct
      601 ctgggcgcgc aggcgcaaac ctttgaccag ctcgacgccc tggtggcggc agtgcgccag
      661 gcggcgcgcg ccggcgacca tgtgctggtg atgagcaacg gcggctttgg cggcgtgcac
      721 ggcaagctgc tggcggcgtt ggctgactga tggaacacgc agaaaacgcc atgccagccg
      781 ccttgagcga cgccagcctg cccctggtca gtattggcat cgtcacctac aaccagaaag
      841 actttctgcg cgagtgcatc gaatccgccc tggcgcagga ttacccccat ctggaaatcg
      901 tcgtggccga cgatggctcc aaagatggca cgcatgagat gttgcgcgac tatcaggcgc
      961 gttaccccgg caagtttgtg ctgcggctgg ccgagcgcaa ccagggcatc accgccaatt
     1021 ccaacgccgc acactttgcc tgcaccggca aatacatcgc ctggatgggc ggcgacgatc
     1081 tgctattgcc cggcaagatt cgccggcagg tggaattcat ggaagcccac ccggactgca
     1141 cgctgtgcta ccacaatctg gatgtgttcc agtccgacac cgatcagacg ctgtttttgt
     1201 tcaacgcccg ctttcacccc gaaggcgatg tgcgcaccgc catccgccac ggcaccttca
     1261 atggcgcctg ctccaccatg gtgcgccgtg acaaaacccc ggcacgtggc ttcaacccgg
     1321 ccattccgat ggcctcggat tggctgtact gggtggaaac cctgcaccac ggcggcagcg
     1381 tgcgttatct ggatgaagta cttggccgct accgccgtca tggcggcaat gtcaccgcca
     1441 gcggccagca ccggctggcg caaacccatg tcgatcacct gagcagttgc cagtacattc
     1501 tggcgctgga tatcgactac ctcgacgacg tcatgcacgc ctacagccaa aagctgctgg
     1561 cgctgcgcca gcacgcccac tttgccaaat ttgcctggta tagctttgtg ctgtcgccca
     1621 gcctgaaggg cgcggtgcgg ctgctgttgt actacatcag cctgggcaaa attcgtaaat
     1681 aagcgcnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnngcca gcgcctgcaa
     1801 cacggcaatg cccaccagca ccgcgctgaa ctgaccggcg tccatccagc cggcaaacag
     1861 cagcggcgcg caggccaggc ccaggtccag cccggaatag acaaagccat aaatccgccc
     1921 gaacgccgac tggccaaagc gcgcggtggc ggcacggcgc accagcaaat cccgcgatgg
     1981 cccggccacg ccggtaaaaa accccatgcc ggccatcaag ggcagcatgc tccagcccgg
     2041 cagccactgt ccggccagca gcgccgccag cactgccgcc aggcccagcg cggcggcaat
     2101 caggtgatca tgggcgtttt tctgcgccag aaacccaccc agaatcaccc cggcggccga
     2161 gcccagcata tacaccgaca aggccagcgc tgcggtgggc cgcgccaggc catacacggc
     2221 gtgcagaatc gggctgacaa agttctggat ggcgccaaag gcggcggcgg taaacagaaa
     2281 aaacaaaaag cacagccaca ccggacgcga gcgcaaaaaa tccagcgtgg acgcccgcgc
     2341 ctccgtggcg cggctggcgc catggcgcgg gtcggcaatc gccgcgtgcc gccaggccag
     2401 cgccagcaaa cccaacaagg ccactaacac cgcgcccaag gccgcctgtc gccagccata
     2461 ggcgctggcc agcgtggtca acagcaaggg cgcgcaggcc cagcccagat tggccgagag
     2521 gccatgcgca gaaaacgcat gccccaagcg cgctggcgac acatgacgat tgagcacggt
     2581 gaaatccgcc gggtgaaaca cgctattacc cagcccggcc atcgccgcca cggccaccag
     2641 catcggcagg ccagtagcaa agtgcagagt cagcccggcc agcacaaaac aactgatgcc
     2701 gccggccagc acccgcgccg cgccaaagcg atccaccaga aaaccagcca tggcctggcc
     2761 aacgccagac accacaaaaa acaccgtcat ggtcacgccg atggcggcaa agcttaagcc
     2821 aaactcggcc atcagccagg gaaacagcgg cggcaacagc aaatgaaaaa aatgcgacac
     2881 accgtgcacc aaaccaatca ggctgatgac ttccaggtcg cggcggccgt ccagcggcgc
     2941 agaaagcgta ttcatggcac aagccttgtt ccatccaggg ggacggccag tctagcccgg
     3001 ctggcgcctt gactggaact cgataagcag acaagaattg tcgccatgca gacatacccg
     3061 gaatgcgacc cactcccgcg acgcccgccg acggcggacg agcccgtcat cgtcaacctg
     3121 cggcatttgc cgccggatgt gcgggtgccg gcgcatcggc acgactgggc gcagctggct
     3181 tatcccttgc gcggcgccat gcggctgtcg gccgccggca tggtgtggct ggcgccagcc
     3241 ttgcgcgcgg tgtggattcc gccgcagatc gagcacgaag tggtggtgtt gggcgaggtg
     3301 gagctgcgca cggtgtatat cgcccccgac gtggcgccgc tgtcattgtc atcgtgcgca
     3361 gtgattgaag tctcggattt aatgcgcgcg ctgttcgagg cgctgagcga cacccagcca
     3421 gacagcgccg acgcgccgct gcgccgccaa cagctgaccg cgctgctgct gaccgaaatc
     3481 cgccgcgcgc cgcccttgtc gctgggcttg ccgatgccgc aggaccgccg cctgcacgcc
     3541 ctgtgtcagg cgctgatcgc ccagccggcg tcgccgctga cgctggccga gtgggcggcg
     3601 cgggtggggg ccagcgagcg cacgctggcg cgcgggtttc agcgcgaatt gcgcatgagt
     3661 tttgccgcct ggcgccagca actgcgcctg gcgcacgcgc tggacggcat cagtcgcggc
     3721 cggccgctgg gcgaagtggc cagcgagctg ggttacgcca gccagccggc gttttccgcc
     3781 atgttcaagc gcgcttttgg cgtgccgccc ggccagtttt tgcgccagca ccgcgcctga
     3841 acatggccac agcatggctc aggccttcag ccagcgcgcg gccgccagct caatgcgctg
     3901 cgggtaatcc gggcattcgg ccgggccaat cagcggcaag gccatctcgg ccaccaattc
     3961 agccaccgtg tcggcgttga tggccagtcc gcgctgacgg gcacggtggg cgatgaccat
     4021 ttcctgtccc cagaaataca cccggccagc gccggtattg gcgcacatgc ggcgcacttc
     4081 tgcatcgcgc tcggcgccaa acagcggcag ccagatatcc gccagcaagg tatctgccgg
     4141 cgtgtccggc acatactgat aggcatcgcc ctgcaccacc cgcacccgcg cacgggcaga
     4201 ttcgggtaat tggtcaaaaa tcccggactg gctgacggcg gcaatcactt ccgggtcgaa
     4261 ctccaccacc gtcacctggc tgaccgccgg atgcagcgcg gcattggctg ccgcccagcc
     4321 catgcccagc cccatcacca ccacatgccc tctggcgtgc tgacagccga tttcctggct
     4381 ttcgatttcc atcgggctca tcgacatcca ggtatgcggc ttgccgcttt cgtcgatgcg
     4441 ggtcagcagg gccatgccat tcaccagttg cggcggtgtc caatagcccg gacacagcac
     4501 catatcggca tggcgcaggc tccagcgcgc gccgctgaat ggctgataac ggggaatgaa
     4561 catcggactt tgatactgag gcaggtcggg caatggggca gcgtgggaca tgacgatctc
     4621 ctgaggcagg caaaggcaaa tgccagccac attagagagc gccacccctg gctgtcaatt
     4681 gcgcggcatt gcaagcgcac atagacaaac agccttgccg tgaaacacgg gcaaggctgt
     4741 ttgtcagtca tcgggcaagt tggtgcgccc agccggggtc gaaccggcac gaccgaagtc
     4801 gaggaatttt aagtcccttg cgtctaccaa ttccgccata ggcgcaccga acctaagatt
     4861 atgccagatc acggcggctt cgtcatccgc cggataaggt caaccgggcc tcacgatgtc
     4921 accaccccga catagcgcgc catattctgc tgcttggcct gggcaatcga ggccagcgcc
     4981 tcgatgcgct ggtgggtggc gtccacctcg gccgccgcct ggcaggcgcc gctggccacc
     5041 cgctccagat ggccggccag cgcatggcac gcctgggact gggtttccgt gcgctcggcc
     5101 atatcgtgaa tatggccggc cacctggcgc acgccgcgcg tgatgcacac cagcgcatcg
     5161 gccatggcct ggccttcggc ggcggcgcct tccaccgcgt cgcgcgagac ggtcattgcc
     5221 gtgcgcgccg cgcccagatc atcgtgcacc tcgctggcca gccgggtgat gtcctgggtg
     5281 gattgccggg tgcgctcggc cagcttgcgc acctcatcgg ccaccacggc aaagctgcgg
     5341 ccggattccc ccgcgcgcgc cgcctcaatg gcggcgttca gcgccagcag attggtctgc
     5401 tcggcaatct cgccaatcgc ttgcgacagc tgctcaatgc cggccagccg ctgggcaaac
     5461 acggcaaaat gcccgccagc gccggccacc gcctgttgcg actcatccag cgcgcgcatg
     5521 gcgcgcccgc ccatctcgct ggcctggctg gccaacaatt cagcatcggc ggcttcatcg
     5581 ctggcctggc tggcggccag ggcaatgtgg tgtacatcct ggctgatggc ctcgatctgc
     5641 gcgctggcgg catgcaccgc ttcggattga tcacgggttt gcgccgacac gctggcgccc
     5701 atgccagcca gcgagcgggc gttgtcgtcg atcacctgcg cctcatgctg ggcgcgcgtc
     5761 agcagggtgg ccaggtcatc gacaaaatga ttgaacgcgg ccgccagctg gtcgatttcg
     5821 tcgcggccct gattgggcag ccgcaccgcc agatcgccag ccgccagcgc gcgcgcatcg
     5881 cgcgccaggc ccgccaagcg gcgcgacaag cgcccggcca gccaggtctg gctgccgaca
     5941 atcaacagcg ccgacacccc cagcggggcc agcacgctcc acagcaattg ccgcagcccg
     6001 gcatccgccg cctcggcatc ggcgcgcgcc tgcgccgaca aggcgttgct cagcgcatcc
     6061 agctggcgca gcatcggttg caggtgcatg gcgtagattt gttcgggaat cgccagcgcg
     6121 tcggccggcg agctggcggc aatgcgcacg gcgctgtcaa actggcgcac atactcgcgc
     6181 cagggcgtca acagcttgct ggcggccggc tcggcgcgcg ccacgctgtt cagcgcgcgc
     6241 gtcacctcgg cgctggtgcg ggtgagcagg gcggcggtgt cgggcaacac cgggtcggcc
     6301 ttggccagct ccagcgcggc ggctttggcg gcagtcagct gatgcacggc ggcctggctg
     6361 gactgagccc gttccagggt gtggcggatg tccgccagtt gccaggcgca ggcggccatc
     6421 accgccaaca gggtgagcgc cgccagcagg gcctgggcaa ataaacgagt ggaaaccttc
     6481 atggggcaat gttggcgtga gtcagacacg gccgccatct tgcgcgccga agattgcaga
     6541 aacatgaaat gacatttaat tgcaatatgc atagcccgac gccatgctga ccacgtcata
     6601 tccaacctcg atgtgtccgc acgccaccac cgcaatcgcc acgctaagcg cagcgttcct
     6661 ggtgaggcgc gcggccgcag acagtacaac aaggccgggc aacaacgcca ggagggtttg
     6721 gtgcgcgccg cgctaactgg cgctcaacgc cgccaccgtc ttcaccgcca cccgtggcgc
     6781 cagcgcgcgc agcgccagaa tgtgcaggat ctcgctgacc gtggtgctgg cgtcgcccag
     6841 cacggtgacc ggatagcgcg cggcggcagg cgatagcgcg gtgtgggtga cgcagttgtg
     6901 cgtcatcatg ccgcacagcc acaggctatc cacgcccagc gcatccagcg tggcttgcaa
     6961 gtcggtctcc agaaaactgt cggcgtgggt tttgatcacc actggcgcct ccggcgcggc
     7021 ggccagcagg cgtgggtgaa tggcgacgcc gtggctgtcg cggttaaaaa acggcgccgg
     7081 gccggcggcg atgtgctgca ccagaatcac cggcacatgg tgggcgcggg cgtgggccac
     7141 ggcggcttcc acctggctta gcgcggcgtc ggcctgccac agcggaaaag cgccgtcggc
     7201 gaaataatcg ttttgtacgt caatcacaat cagggcgggc tgcttcatgg cggggggttt
     7261 cctcagggtg gtggtgagcg cccatcatcg cagcgccagc ctgcacccgg cagtgacccg
     7321 caggacagta aacggtaaga tcgggccaat ctgtattgcc ctggcaggag gctttatgcc
     7381 ccgcgtgcat cccgtggcca tcctggcctt tgaccgcatc agcgcctttc atctggccgc
     7441 gccgtgcgtg gtgtttggcg aggcctgccc gctgccggaa tgctttccgg tgcaggtgtg
     7501 cgcctgggag gccgggccat tgcgcaccac gtcgggcttt accctgagcg tcgagcacgg
     7561 gctggaagcc ttggcccgtg cggaagtggt gattgtgccc agttggcgcg acccggccga
     7621 gcgggcgccc gaagcggtgc tggatgcctt gcgggcggcg catgcgcgtg gcgcgcaggt
     7681 ggtggggctg tgtctgggcg cgtatttgct ggcgcaggcc ggcctgctgg acgggcgcgg
     7741 cgccaccacg cactgggcgt atgcggcgga ttttgcccgc cgtcacccac aggtgcggct
     7801 gaacgccgat gtgctgtatc tggctgatgg cgcgctgctg acttcggccg gcacggcggc
     7861 ggcgctggat tgctgcttgc agctgttgcg cgagcgcctg ggcgcggaag acgccaaccg
     7921 cgcggcgcgc cggctggtga ttgccccgca tcgcgctggc ggccaggcgc agtttatcga
     7981 gcggcccttg cccagcacgc gcgacgacgc ccggctggcc ggcctgctgg acgaggtgcg
     8041 cgcccagctg gatgcgccgc acagcctgga tgcgctggcg gcgcgggcct tgatgagtcg
     8101 gcgcaccttc acccggcgtt ttcgccagtt gaccgggcaa tcgtttcagg cctggctctt
     8161 gggcgagcgg ctggcattca gccagcgcct gctggaagtc agcgcgctgc ccatcgagcg
     8221 gattgccgcg ctggccggct ttggctcgcc cgaggcgttc cggctgcatt ttcgccgcag
     8281 ttttgctgta tcgcccagtg cttggcgaca ggcatttact ggcggctgag cgcggctata
     8341 cattgcgcgc cagctggcgc agggtggcca gcaccacctc gccccgctga tgcagttgcc
     8401 ccagttcgcc aggcgccggc tgcgcgctgg cttgacacag ctgacggccg aaggcatgca
     8461 gctcggcgtg gacctcgcaa aggtgaatga acaggggctc ggcgccatgc cgacgtgcgc
     8521 cttcgttcgc cagccagtgg gcaaaccggc tgtgcgcctc atccagggtg ggcgcgctgg
     8581 cgcgctcgcc gcgcagatac agggtcacgg cggtcatcca ggcgcgcagc tcggtgcggg
     8641 caaacagcaa gggcaggtca tcacggttca gtgccggcaa attgcgccag ttggggtcac
     8701 cctgccagtg ggcggcccag gcgggcagct tgctggccgg catgggccgg gcaatgccat
     8761 aaccctgggc ttcttcacag cccagttgca acagcatggc gccgtgttcg gcggtttcca
     8821 cgccttcggc gatcacatcg cggcggaaag ccgccgccag gccgatcacg ccactgagaa
     8881 tggccagatc gtccgggtcg tccagcatgt cacgcacaaa gctctggtcg atcttcaatc
     8941 gagccacttg caggtgctta aggtaagtca gcgaggaata gccggtgcca aaatcgtcca
     9001 gggcaaactt gacgcccatg gcgcggcagg cagcaatcac ctgggccact tcggccaggt
     9061 cttccagcgc gctggtttcc aggatttcca gctcaagatg ctcaggatcc acctcggcgt
     9121 ggctgtccag aatttcgcgc agtcggctga caaaccgggc ttgctgtaac tggcgggcgc
     9181 caatattgac gctgaccggc aggatcaggc cctgtgcctg ccacaggctc atctgcgtca
     9241 acgcctggtc gatcacccat tcacccagat ctaccgccag cgggtgggca tccaccagcg
     9301 gcaggaaggc ggcggggggc agcaggccct tgtctggatg ctgccagcgc aacagggctt
     9361 cggcgccaat cacttcgccc cggcgcatgt tcaccttggg ctggtagtgc agcacgaatt
     9421 cacccgcttg cagcgcccgg cggatgcgat ccaggctttc gtgctgcccc ttgatgtggt
     9481 gatcgtgggc agcatcaaac aggtgatagc ggtttttgcc ggccagcttg gcctggtaca
     9541 tggcctggtc ggcctgacgc atcagttggt cggcgtccac ctcgccttcc tgcgggtaaa
     9601 acgtcacccc caggctggca gacacttgca gtggctggtt gcccagcatc accggctggg
     9661 cggcagcggc cagcaggcgg gtcagtgctg gcgcgctggc ctgggtgtcg tccagatcca
     9721 tcagcacggc aacaaactcg tcaccgccca gccgcgccag ggtatcgctg tcgcgcaggg
     9781 tgtcgcgcat gcgccgcgcc agctgcatca gcaattggtc gccaatatcg tggccatggc
     9841 ggtcattgat ggccttgaag ccgtccagat ccagatacac cacagccagg cgttgactgc
     9901 gccgctgcgt ctggctcatg gcctgttgca ggcgatcggc cagcagcacg cggttgggca
     9961 ggccggtcag cgcatcgtag tgggcaatat gctccagctg ctgctcgtgc gccttgatca
    10021 gggtgatgtc agagaacagc gccacatagt ggcgggtatg gccatcttcg tcgcacactg
    10081 cgctgatggt ctgcatttca gcgaagattt cgccattttt gcgctgattc cagatttcgc
    10141 cataccaatg gcccttgtcc agcaggctgc gccacatggc ggcgtaaaac tcgcggtcgt
    10201 ggcgaccaga acgcagcatg cgcgggtttt gccccaaggc ctcgtcacgg ctgtagccag
    10261 tgatccgggt aaaggtgtcg ttgacgtcaa tgatattgcc ttcggcgtcg gtaatcatga
    10321 tgccttcgcg ggcatgcaca aatacgctgg cagccagttg cagctgttgc tcggtgcgcc
    10381 ggcgctcgct gatgtcgctg agcgaattga gcatggccgg tcggccctgc caattgatgg
    10441 gcgtgctgct catctcgatc cagcgcggcg attcgccatt gcccaggcga aactgacagc
    10501 gcggcacccc atgaccactg ctgccgggca ggggatgcag ctggcgggcc acctggcgct
    10561 ggtcatcggc atgcaccagg gcgagaaaac cctggccata caggccggca gggccagcat
    10621 tggccatgtg ttccatggtg ggattgcagt agcagatgcg tccatcctgc accaccacaa
    10681 tgccttcatg tgccagctcg atcaggctgc gatagcgcgt ttcgctctcg gccagctgcg
    10741 cctcagcctg acgacgctca atggccaggc tgatcagccg tgcgcattgt tcgatcagta
    10801 caatatcggc ggcggtcggg gtatgtggtt ggcgatgata gatggcaaaa gtgcccagca
    10861 cctggccatc agccgacagg atgggctgcg accagcaggc ggccagcccg gcctgacagg
    10921 ccagctcggc ataaggcgcc cagttgggat ggtgggcgat atcagccacc accacacgct
    10981 ggccgcgatg ggccgctgca ccgcagctgc ccacatcagg gccgattttc aggccttcga
    11041 ctgcggcgtt gtaaaatgct ggcaagctgg gagcaatgcc ctggcccagg cagccggttt
    11101 gcgaattgag cagcaggatg ctgcacagca tggccgggtg catctgctcg acttcgtgca
    11161 ccaccgtcat cagcaaggcc ggcagcggct cgccgcgtgc cagcaaatcc agcacccggc
    11221 tgcggaactg ctcgtggcgt tcggccagat ggcgctcgct gatgtcggta tgggtgccga
    11281 tcatacgcag tggttggtca tctgggccac ggctgaccac catgccccgg ctcagtatcc
    11341 atttgtagtc accctgcttg ctgcgcaggc ggaactccag ctggtagtgg ccatggtggc
    11401 catccagata ggcggcaagc ccggccagtg cagcagtacg gtcttgcgga tgcagcagat
    11461 ccagccaggc ttggtaatgg gcgggcaact cgccgggggc gtagcccagc atgtccagcc
    11521 agccacgcga gtactccacc cgattatcct gcaaatccca gtcccacatg ccgtcgccag
    11581 tgccttccag cgcgcattgc cagcgaaact cgctgtcgcg cagcgcctgg ctgtgcatca
    11641 gcacacgctg gcgctcaatc gccagcgagc gattggtcag ccacaggcgg aacagcaaac
    11701 cggcaatcac cagcagcccc agcgccccca gcaccacaga caaggcataa cgtgcccaga
    11761 tgtcgctcag ggtaaaggca gggccgcgat caaacggcgg catgcgcaag gtcagcagca
    11821 ggtgctcaat gctgccgtag tcggcaggaa tggtgaagcc atgaatcccc atggcacggg
    11881 tcagcggagc atcgtgtggc aattgcagca gcgccaccgc cacccgtttg gccagtgccg
    11941 cgtccacacc ggccagcgca acaaatggcc attccggata cagctcggtg ctggtcagcc
    12001 agggcaactg gccatcgggg gtatcaggca gatgcatcac ctcgatgtct tccagccgca
    12061 gccggccttc accagccagc tcttccagca agcccgtgcg caccaggccg gcatcggcac
    12121 ggccgctaag cacagcctgc accaccttgt cgtgcggcgc cccggtggtc agccacaggg
    12181 tgtcacgctt gagatccagc ccagcctcgc gcaaggccag cgcctgcatc tgatagccgc
    12241 ccagcgacat cgtgctggta aaagccaccg ttttgccatg caggcgctcg ggcgtatcca
    12301 gcccgcgccc gcgcaacgag aacaccaccc cgccaaagct ggcgctgggt gtgccattta
    12361 catcgttcac cagggtggcc agcggggcag acaatttttg ctggtggctg atcagcacga
    12421 aatgcccggg attggtcagg acaaagtcta cctgcttgcg cgcgacggcc gcttccagct
    12481 cgggaaaact ataggcttcc acgcgaaaat cccgctcggg aatcgcctgc ttgagcgcct
    12541 gcgccagcgg ttgccattgc gccagggttt gtgccttggg gcgataggcc agcacgccaa
    12601 tacgcaccgg ttcggccgca gtagccagcc agcacagcgt gcacagccac aaggacagga
    12661 ccatgcggat catgcgagag aaatcaagca tattgcaaac caatatactg tgttccgcga
    12721 taaaattcac cgcctgtcag caaggggacc gcaaaaaatg tccggccctt caacagctga
    12781 actgcgccat ggcctgacgc atgcggctgg cggcttcggt cagagcctga ctggtactga
    12841 ccgcatcgcg caccagccca tgattttcac tggccagcac ggcaatatcg cgcgaatggc
    12901 gcaggatgtc ctgcgctgcc ccggactgct gctctgcagc atgggccagt tgctccatgg
    12961 attcaaacga ggcgcgcgag cgctggctga gcgtggccag cggctcgaca atgctttcca
    13021 cctgcgctac cccctggcgc acctcgccca ccccggcgtc catcccctga acagcggcac
    13081 gggtttgctg gcgcatggcg tcgatcatct ggtgaatctc atgtgtggac tgggtggcgc
    13141 gggtggccag cgcgcgcacc tcgtcggcca ccacggcaaa gccgcgtccc tgctcgccgg
    13201 cacgggccgc ctcaatcgcg gcgttcagcg ccaggaggtt ggtctggtcg gatagttcac
    13261 gaatggtgtc cacgctgcca gcaatgctgt ccgagcgttg ctgcacagca ttgacctcgc
    13321 gggccgaggc cgccactgca tcggacaagc gccccacatc gcgggcagca tcgcccacca
    13381 gcccacggcc atgttcggcc aacgccacgg cctcttcggc caggctgcgg gtatgctggg
    13441 cagtgtcggc cacactatga gcctgctgat tcagtgcctc caccgccgtg gcaatggtgc
    13501 tggccatacg atcctgcgcc tgtgagcgct gggcaatttg ttcagcagca cgcgccaggg
    13561 tatcggccga ttcacccacc tgcgccgcgc cagcattgac ggcgccaatc atctcgcgca
    13621 ggctgccctg catgcgcgcc accccgcgca gcaacagggc aggctcgtcc cgcccctctg
    13681 cctgcaaggg ggtgcgcaaa tccccggcgg ccacccgctc cactgctgca ccggcttcat
    13741 gcaagggccg cacaatgctg cgggtgatgc gccaggccag cagcacgctg atcagcagcg
    13801 aagcgccgcc cagcgccagc atggcttgct tggcctgctg tgagcgggca tcggcgtcct
    13861 gcgcaccctg ctcggcccgg tgggtttcca gctccaccac ctgacggctg gtcgcaacca
    13921 ggctatccag cgccggcagg gtgcgggttt ccagcagttt ttgcgcttcg gcccgttcat
    13981 cgccttccag caaatccgcc gcatcgacaa aaatctggtt atacgcctga ccctgagctt
    14041 taacctgctc cagcagcgtg gtttcctgcg gggtgtcggc cagcggcagc aactgcgcca
    14101 acgccttgtc cagcaccgcc ttgttggcgt cgatatcttt gtacagagga atgcgcgcct
    14161 cctgactggg cgcggccacc agccgcacca gcttcatggc attggcggcg gcggtttcca
    14221 tcaccgtatg cgccagttcg gttttgacca gatcgcgccc gacaatctga cgcgcgccag
    14281 cctgcatggc attgatggca agggtggcag cgccggcaat cagcgccatc agcatgacca
    14341 gcacggcaaa gccggcgccc agacgcgggc cgatggtgag ttggcgtaac acgaagttct
    14401 cctgcataaa gcaatggtca agaatcggga gcgaacacag cgcgccgggt caggcgatgg
    14461 cggcgtatgt ccttttggtc gcctcagcgc tgacccacct tacacgggct gcacaaaaaa
    14521 atcttgaact catgcttgaa tcatagtgag gccgccgcgc aattgccgac agacgcaaca
    14581 aaggccgcct accgggatac ggcaagcggc cttggcaggc atgctcgatt tagacgggaa
    14641 gctcagtcag gaaacaagcc agtggacaaa taacggtcgc cacggtcgca gacaatcgac
    14701 acaatcaccg cgttttccag ctgttccgac aatttcagcg ccactgccag cgcaccgcca
    14761 gacgatggcc cggccagaat gccctcttcg cgcgccagcc ggcgcactgt gtcttccgcc
    14821 tcggcctggc tgaccaactc caggcggtcg atacgggaaa aatcataaat cttcggcagg
    14881 tattccggct cccacttgcg gatgccggga atctggctgc ccggctctgg ctgcacgccg
    14941 acaatctgga tggccgggtt ctggcttttc aggtagcgcg aggtgcccat gatggtgccg
    15001 gtggtgccca tgctggagac aaaatgggtg atggtgcccg cggtgtccgt ccagatttcc
    15061 gggccggtgc tgtcaaagtg cgcttgtgga ttgtccgggt tgccaaactg atccagaaaa
    15121 cgcccttcgc cggcttcaat ccgccgacgc gcctcgtcaa tcgcgcccgg catcgacgcc
    15181 gacgccgggg tgagcaccac ttcggcgcca taggccttca tggtggccac ccgttcggcg
    15241 ctggagtttt ccggcatcac cagcaccata cgatagccca gcatcgccgc cgccatggcc
    15301 aggccgatgc cggtattgcc gctggtcggc tcaatcagcg tgtcgccggg gcgaatatcg
    15361 ccgcgcgcct cggcgcggcg aatcatcgac aaggccgggc ggtctttcac cgagccgccg
    15421 ggattattgc cttccagctt gaccagcacc acattattgc gcccgcgcgc catgcgcttg
    15481 agccgcacca gcggggtgtg gccgacaaag tcttcgaggg tgtgaaaagt cggggggatg
    15541 gacatcggcg gcttcctgaa actgaaaaag agttcagcgg attatagccc tggccgccgc
    15601 cgtggcgcgg ggcgaacaca gaatggtttt ctatatggtt agaacgccgg gctcaggcgc
    15661 gcagcgccgc caccagcgca tgcatccacc cgcaatcttc accacgcggg tgccgggcat
    15721 caatgatgaa ctgtaaattt ttggccgtgg tttccccctc ccaaggctcg cgccgtcggg
    15781 ccagcgcatc cgccagtgcg gtttgcgcgc cgatgagatc gcgcgccagc accgccagct
    15841 ccagttgggt ggcgtaatcc cagtaatccg gctggccgct ggccatgcgc cgcgccaccg
    15901 cgtagcgcac caccggtaat agctcggcct ggcgcgggtc gggcgtctct tgcatctcca
    15961 tcagcgtcac cgcgttcacg cccggatagg catcgcgcca gtcggcctca aagccgcgca
    16021 ggtattcggc aatcgccagc t
//
LOCUS       DAOPYX010000027        15570 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_4068_length_15570_cov_11.605930, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000027 DAOPYX010000000
VERSION     DAOPYX010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 15570)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15570)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15570
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4068_length_15570_cov_11.605930"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(52..558)
                     /locus_tag="PKN55_02600"
     CDS             complement(52..558)
                     /locus_tag="PKN55_02600"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HNX70166.1"
                     /translation="MTMQDKIYHVLFICTGNSARSIMAEAVMNQLGQGRFRAYSAGSL
                     PRGEVHPLTLSTLAEQGYPTAGLSSKPWEVFAGPDAPAMDFVFTVCDQAAGEACPVWP
                     GQPMTAHWGFADPAAQVSDDPAQARKAFTAALAQITTRIRLFLSLPLDKLDRLSLQAH
                     LRELGRPA"
     gene            complement(555..2324)
                     /gene="arsA"
                     /locus_tag="PKN55_02605"
     CDS             complement(555..2324)
                     /gene="arsA"
                     /locus_tag="PKN55_02605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008954211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015446 - ATPase-coupled arsenite
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0071722 - detoxification of
                     arsenic-containing substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenical pump-driving ATPase"
                     /protein_id="HNX70167.1"
                     /translation="MLCLHHAPRHLFFTGKGGVGKTSLACACALWLAEHGRRVLLVST
                     DPASNVGQVFGVSIGNQITALPAAPGLSALEIDPPAAAAAYRERMLAPVRGVLPDAVV
                     AGIAEQLSGACTTEIAAFDEFTALLTDGALTAGFDHIVFDTAPTGHTIRLLQLPGAWS
                     EFLDNAHGDASCLGPLAALDKQREQYQAAVATLTDPARTRLVLVARAQRASLREVART
                     QHELSAIGLPHASLVVNALMPAAEAQRDPLAAAVHAREQAALAAMPETLASLPRDTLP
                     LLPDNLVGLDALRALLAQTAPALPPAGEPTPLPAELAARSLGALVDDIAADGHGLVML
                     MGKGGVGKTTLAAAVAAELARRGLPVHLTTSDPAAHLSDTLAGSLPGLTVSRIDPVAE
                     TARYRQHVLDSKGAGLDADARALLEEDLRSPCTEEIAVFQAFSRIIREAGRQFVVMDT
                     APTGHTLLLLDATGAYHREVARQMGDARYTTPMMLLQDPKRTKALIVTLAETTPVLEA
                     AALQADLRRAGIEPWAWLINQSVAAAMPSSALLQQRARHELTELAHIAERHAGRLALI
                     PLLAEEPVGPTRLYALTHPESGA"
     assembly_gap    2386..2485
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2644..3003)
                     /gene="arsD"
                     /locus_tag="PKN55_02610"
     CDS             complement(2644..3003)
                     /gene="arsD"
                     /locus_tag="PKN55_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000855179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite efflux transporter metallochaperone
                     ArsD"
                     /protein_id="HNX70168.1"
                     /translation="MKTLQVYDPALCCSSGVCGVDVDQTLVDFAADLNWLKQQGVAVT
                     RFNLAQQPLAFAQEAAVKAALDAGGAEALPVLLVDGQLRGQGRYPSRAELAQWLALTP
                     EALPVAPAACCSGSRCC"
     gene            complement(3051..3530)
                     /locus_tag="PKN55_02615"
     CDS             complement(3051..3530)
                     /locus_tag="PKN55_02615"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014087222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HNX70169.1"
                     /translation="MNILFLCTGNSCRSILAEATFNHLAPAGWRALSAGSQPTGEVHP
                     RSLALLAAEGIDASDCASKSWDALPVTPDIVITVCASAAGETCPAYLGPVLRSHWGVD
                     DPAKATGSPADIDAAFVRAYRVLRARIEALLALPAEQLHGERAALKAELDRIGQLSA"
     gene            complement(3531..3923)
                     /locus_tag="PKN55_02620"
     CDS             complement(3531..3923)
                     /locus_tag="PKN55_02620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014003887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNX70170.1"
                     /translation="MDKFTAAKLFESLSSGVRLDVYRLLVRHGLDGLVAGAIATELDI
                     PPTNLSFHLKALTQAGLLTVTQEGRFQRYRANIALMLELIAYLSAECCAGQPEQCASL
                     RAASPCPEALLPPLAGAHPTCSDPACEP"
     gene            complement(4004..4774)
                     /locus_tag="PKN55_02625"
     CDS             complement(4004..4774)
                     /locus_tag="PKN55_02625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018153179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HNX70171.1"
                     /translation="MQLATWNVNSLKVRLPQVLDWLAANPVDVLCLQELKQEDHAFPL
                     EALAAAGYRAEFAGQKTYNGVAIVYRDTLTATEVIKDIPDYDDPQRRVIAASFGELRV
                     VCAYFVNGEAVDSDKYAYKLTWLGKLERYLADSLARWPQLALLGDFNIAPDDRDVYDP
                     AGWAGKVLCSAPERAAFQRLVAMGLADSYRHFHADGGQYSWWDYRAAMFRRNLGLRID
                     HILLSPPLLARAQSCEIDRAPRKNERPSDHTPVVVTLG"
     gene            complement(5254..>6385)
                     /locus_tag="PKN55_02630"
     CDS             complement(5254..>6385)
                     /locus_tag="PKN55_02630"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HNX70172.1"
                     /translation="SAAALSLPALAAGKPVFPTLNGQPPLIIAHRGASGYLPDHTLEG
                     YAKAIELGADFIEPDLVMTKDGVLIARHEPNLKDTTDVARRAEFASRKRKMMVDGVEE
                     EGWFASDFTLAEIKTLRAIQPRADRSKAFDGQFQIPTFDEILALRAQKSKETGRQIGV
                     YPETKHPTYHQQLGLSLEKPLVALLAKYQLNSQKAPVFIQSFEAANLKQLRKLTSNKL
                     VYLLDAETVRPDGVVERKMPYDHVLSGDKRSYGDMLSPANLKQVKTFADGIGPWKPYL
                     ISWQAMTDPKTGKPIDVNGDNVIDQRDMTLLEANDVVKRAHQLGLVVHPFTFRNEAKL
                     LAANFQDNPAEEYRLFFELGVDGVFSDFTDTAVATRTLMQMR"
     assembly_gap    6386..6485
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(6624..7544)
                     /locus_tag="PKN55_02635"
     CDS             complement(6624..7544)
                     /locus_tag="PKN55_02635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019923930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNX70173.1"
                     /translation="MMDSMIPVLAGTLAAATPLIFAGLGELVAERSGVINLGVEGMIL
                     IGAIAGFAAAAETGLGPAAGLPAGALAGMGAALVFAVFALSLAANQAACGLALTIFGI
                     GLSAFIGQHYVSYSLPGLKPLSIPLLSDIPVIGPVLFHQDAVVYLSLAAFALVAWTLA
                     KTRLGLLLKAVGESPEAAHAIGYPVLAIRYGAVLFGGAMAGLAGAYLSTVYTPLWVQN
                     MSAGRGWIAVALVVFAAWKPGRLLLGAYLFGAVTILQFHAQGLGIALPVQLLAALPYL
                     ATIVVLVLISRDRRLLQLNLPASLGKPYLP"
     assembly_gap    7599..7698
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(7823..8878)
                     /locus_tag="PKN55_02640"
     CDS             complement(7823..8878)
                     /locus_tag="PKN55_02640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018149834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNX70174.1"
                     /translation="MHLLEPRPQPSQLMRWTAPLIAIVATLLVGSGLFLALGKDPLQS
                     FKVFFVEPLTSSYGWSELLIKACPLILIAQGLAIGFKARIYNIGAEGQLIVGALAGGG
                     IAIQFGDSGAAWVLPAMVLAGALGGALWGAIPALLKTRFNAEETLTTLMLAYVASFLL
                     SYMVNGPWRDPEGMNFPQSVMFADGALFPILLEGSRVNGSVFITLFAVLGFWLFNAKS
                     FLAYQLEVGGQAPAAARYAGFSAQRTVWFSLVLSGLTAGLAGVGEVAGPVGQLNLNIS
                     PGYGFAAIIVAYLGRLHALGIVLAGALMALIYLGGEAAQVALQLPAAITGIFQGMLLF
                     FLLGADAFIDNRLRRRK"
     gene            complement(8865..10397)
                     /locus_tag="PKN55_02645"
     CDS             complement(8865..10397)
                     /locus_tag="PKN55_02645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019923932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNX70175.1"
                     /translation="MSQTPPRLTLTGISKRYPSVVANDGVSLSVAPGEIHAILGENGA
                     GKSTLMKIIYGAVKADAGEIRWNGQPVTIANPAQARKLGIGMVFQHFSLFETLTVVEN
                     IALALDAPFDPAALALRVREVSERYGLPLDPHRLVHSLSVGERQRVEIVRCLLQDPKL
                     LILDEPTSVLTPQAVQKLFDTLRRLASEGVSIVYISHKLHEIQTLCDSATILRGGKVT
                     GEAIPRDETPASLARMMIGRELPVCEAPPYRGPSRPVLEIRQLSQRSDDPFGTSLSDV
                     SLTVNAGEIVGIAGISGNGQAELLAALSGEAPSGKTTVWLDGEAIGHLHPGRRRARGL
                     AFVPEERLGRGAVPSQSLAHNALLTGHRHGGMRRFGLVSYARARQFAQDCIARFDVRC
                     GGPLAAARSLSGGNLQKFIVGREIMLSPKAMIVAQPTWGVDVGAAAFLRQTLLDLSRS
                     GVAILVISEELEELFEISDRIAVLAQGRLSAAVPKAEADAESVGLQMAGLFNAEASHA
                     SA"
     gene            complement(10400..11401)
                     /gene="xdhC"
                     /locus_tag="PKN55_02650"
     CDS             complement(10400..11401)
                     /gene="xdhC"
                     /locus_tag="PKN55_02650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008954776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0009115 - xanthine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase accessory protein XdhC"
                     /protein_id="HNX70176.1"
                     /translation="MRWLNALLDGLAAGEPMVWVSVARLHGSGPREVGASMVVGAHRQ
                     ADTIGGGHLEWQAIAQARQLLSASTGHPAARVCRYNLGARLGQCCGGVVWLLFEVIAP
                     SALADWQARAAQLSAGQALWRELNAGEAASRWRLAAPTEASSALDGGEQDWRWRQRLA
                     RAPFPVWLFGAGHVAQALVTQLRLLAAQVRWIDPRDDAFAGLDLAGVDARVNDDPASE
                     VAEAPPGCFYLVMSHSHALDFALCEAIFARRDAAYFGLIGSHSKRASFEQRLLARGLD
                     AARLSELTCPIGIAGITDKQPAAIALAVAAQMLQLHNARQWLNQAHTLIQPTVFYPE"
     gene            complement(11389..13713)
                     /gene="xdhB"
                     /locus_tag="PKN55_02655"
     CDS             complement(11389..13713)
                     /gene="xdhB"
                     /locus_tag="PKN55_02655"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008954777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004854 - xanthine dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0009115 - xanthine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdopterin binding
                     subunit"
                     /protein_id="HNX70177.1"
                     /translation="MRADTPPKPVGHALPHESAALHVSGAAHYLDDLPELAGTLHAAI
                     GQAPCAHGRLRALQLDAVRAAPGVRAVLTAVDIPGDNNCGPILHDDPLLAADEVQFYG
                     QPLFVVAADSREQARQAVRLAQIDIEPLPALLDIDSAVAAESWVLPPFEMQRGEVDAA
                     FAAAPHRLSGQARVGGQEHFYLEGQISYAQPGDDDSLHVWCSTQHPSEMQQLIAHALG
                     WRAHQITVSCRRMGGAFGGKESQSAQWACLAALLAVRTGRPVKLRLDRDEDMSATGKR
                     HGFQYQWQAAFADDGRLAGLKLEMASHCGFSADLSGPVNDRAICHLDNAYYLDALALR
                     SLRCKTHTVSNTAFRGFGGPQGMFLIETVLDDIARHLQLDPLAVRQANFYDSEPGGAR
                     SVTHYGMPVTDNIAPALVAELAARCQYAQRRQAIAEFNAASPTLKRGLALTPVKFGIS
                     FNATHFNQAGALVHVYTDGTVLASHGGTEMGQGLYTKIRQIVAGEFGLPLADVRLAAT
                     DTSRVSNTSATAASSGTDLNGKAAQAACQQIKARLAALMVELAGGGSADAVRFADGQV
                     RLADFSLPFAELAMRAYRARVQLWDSGFYRTPDIHFDPVSKLGRPFYYFAYGAAACEV
                     AIDTLTGESRVLRADLLHDVGQSINPALDIGQIEGGFVQGMGWLTSEELHWRADGKLM
                     THAPSTYKIPTAADVPAVFNVALYANANREDSIHRSKAVGEPPFMLALAVFFALRDAV
                     AAALPAGAPVQLDAPATPEAIVRALRAGSGACAG"
     gene            complement(13715..15181)
                     /gene="xdhA"
                     /locus_tag="PKN55_02660"
     CDS             complement(13715..15181)
                     /gene="xdhA"
                     /locus_tag="PKN55_02660"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014085680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004854 - xanthine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009115 - xanthine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase small subunit"
                     /protein_id="HNX70178.1"
                     /translation="MIERPIQFYFRGSLRQIAGPSPTRTVLQYLREDLRQTGSKEGCA
                     EGDCGACTVVLGELDADGGLRLRAVNACIQFLPALDGKALFTVEDLADADGLHPLQQA
                     LIDHHGAQCGFCTPGFAMSLFARYHSQPDCPSRAELEDCLSGNLCRCTGYRPILDAAQ
                     AAWQAPRREWDSAPVAAALRQLAALPPLHYRAADGGESFSPRSLAELAALRQQHPAAR
                     LVAGGTDVGLWVTKQGRALDSLIQLGAVTELKQIAEVDGQLEIGAAAPLHDAFAALSQ
                     WTPHWAELARRFASTPVKHAGTLGGNIANGSPIGDSMPALIALGATLRLQRGDVCREL
                     ALEDFYLAYQRTALTEGEFVRAVRVPRPLGEFRAYKVAKRHDQDISAVAAGMQVHTNA
                     AGQVSLARLAYGGMAATPRRAAQAEAALLGAPWSAASVRAAMAALEGDFQPLSDARAS
                     ADYRRHVAAQLLWRFWLDSQGQAGHRLDALAPISLEGC"
     gene            15374..>15570
                     /locus_tag="PKN55_02665"
     CDS             15374..>15570
                     /locus_tag="PKN55_02665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX70179.1"
                     /translation="MNLVTYLGKQILGVLACYQQDDIVIHICGVYPQWDNRLRVFFPR
                     GHALQVGELVTLHLDNRSGVD"
BASE COUNT         2586 a         5068 c         5208 g         2408 t
ORIGIN      
        1 ggccagacgc acggtcgggc aaccgcgcca ggcgggtttt gttggcggcg cttaggccgg
       61 gcggcccagc tcgcgcaaat gcgcctgcaa agacaaacga tccagcttgt ccagcggcag
      121 gctgagaaac aggcgaatgc gggtggtgat ttgcgccagc gcggcggtaa acgccttgcg
      181 cgcctgcgcc gggtcgtcgc tgacctgggc ggccgggtcg gcaaatcccc agtgcgcggt
      241 catcggctgg cccggccaca ccgggcaggc ttcgccggcg gcctggtcgc acacggtgaa
      301 cacaaaatcc atggccggcg catccggccc ggcgaacacc tcccagggtt tgctgctcag
      361 cccggcggtg gggtagccct gctcggccag ggtgctcagg gtcagcggat gcacctcgcc
      421 gcgcggcagg ctgccggcgc tgtaggcgcg aaaccggccc tggcccagct ggttcatcac
      481 ggcttcggcc atgatgctgc gggcggaatt gccggtgcag ataaacaaca catggtagat
      541 tttgtcttgc atggtcatgc tcctgattcg ggatgggtca gcgcatacag gcgcgtcggg
      601 ccaaccggct cttcggccag cagcggaatc agcgccaatc ggccggcgtg gcgctcggcg
      661 atatgggcca gttcggtcag ttcgtggcgg gcgcgctgtt gcagcaaggc agaggatggc
      721 atggcggcgg cgacactttg attaatcagc cacgcccacg gctcgatgcc ggcgcggcgc
      781 aggtcggctt gcagggcggc ggcttccagc accggggtgg tttccgccag ggtgacaatc
      841 aaggccttgg tgcgcttggg gtcttgcagc agcatcatgg gcgtggtgta gcgggcgtcg
      901 cccatttgcc gcgccacttc gcggtggtag gcgccggtgg cgtccagcag caacagggtg
      961 tggccggtgg gcgcggtgtc catcaccaca aactggcggc ccgcctcgcg gatgatgcga
     1021 gagaaagcct gaaacacggc gatttcttcg gtgcagggcg agcgcaggtc ttcttccagc
     1081 agcgcgcgcg cgtcggcgtc cagcccggcg cccttgctgt ccagcacatg ctgacgatag
     1141 cgggcggttt cggcgacggg gtcgatgcgg ctgacggtca ggccgggcag gctgccggcc
     1201 agggtgtcgc tcagatgggc ggcggggtcc gacgtggtga gatgcaccgg caggccgcgc
     1261 cgggccagct cggccgccac ggcggcggcc agcgtggttt tgcccacccc acccttgccc
     1321 atcagcatca ccagaccatg gccatcggcg gcgatgtcgt ccaccagcgc gcccaggctg
     1381 cgcgcggcca gctcggccgg cagcggcgtg ggctcgccag ccggcggcag cgcgggggcg
     1441 gtttgcgcca gcagggcgcg cagcgcgtcc agcccgacca gattgtccgg caagagcggc
     1501 agggtgtcgc gcggcaggct ggccagcgtc tcgggcatgg cggccagcgc cgcctgttcg
     1561 cgcgcatgca ccgccgccgc cagcgggtcg cgctgggcct cggcggccgg catcagcgca
     1621 ttgaccacca gcgaggcgtg cggcaggcca atggcgctga gctcgtgctg ggtgcgcgcc
     1681 acttcgcgca ggctggcgcg ctgggcgcgc gccaccagca ccagccgcgt gcgcgccggg
     1741 tcggtcagcg tggccaccgc cgcctggtat tgctcgcgct gtttgtccag cgccgccagc
     1801 ggccccaggc aggaggcgtc gccgtgggcg ttgtcgagaa actcgctcca ggcgccgggc
     1861 agttgcagca ggcggatggt gtggccggtg ggggcggtgt caaacacaat atggtcaaac
     1921 ccggcggtca gcgcgccgtc ggtcaatagc gcggtgaact catcaaaggc ggcgatttcg
     1981 gtggtgcagg cgccggacaa ttgctcggca atgcccgcca ccactgcgtc gggcagcacg
     2041 ccgcgcactg gcgccagcat gcgctcgcgg taggcggcgg ccgcagcggg cgggtcgatt
     2101 tccagcgccg acaagcccgg cgccgccggc aaggcggtaa tctgattgcc aatgctcacg
     2161 ccaaacacct ggcccacatt cgaggccggg tcggtgctga ccaagagcac gcgccggcca
     2221 tgctccgcca gccacaaggc gcaggcgcag gccagcgagg ttttgcccac gccgcctttg
     2281 ccggtgaaga ataaatgacg cggggcgtga tgtagacaaa gcatggtgag gtgctccaat
     2341 cagtcaaaat taagcgttgc tgacaaagcg cgcctggcgg tgttgnnnnn nnnnnnnnnn
     2401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2461 nnnnnnnnnn nnnnnnnnnn nnnnngtgct ccaatcagtc aaaattaagc gttgctgaca
     2521 aagcgcgcct ggcggtgttg tacgacaccg ggggtgatgc tcatgccgaa agcggtctca
     2581 tcgccagtca atcggtgccg atcatggcga cgggctacta gccagccgcg ctttgctgag
     2641 tggttaacag caacggctgc ccgagcaaca cgctgccggc gccaccggca gcgcctcggg
     2701 cgtcagcgcc agccattgcg ccagttcggc gcggctgggg tagcggcctt ggccgcgcag
     2761 ttggccatcc accagcaaca ccggcagcgc ttcggcgccg cctgcgtcca gcgcggcctt
     2821 gacggcggct tcctgggcaa aggccagcgg ctgttgcgcc aggttaaagc gcgtcaccgc
     2881 cacgccttgt tgcttgagcc agttcaggtc ggcggcaaaa tccaccaggg tttgatccac
     2941 gtccacgccg cacacgccgc tgctgcaaca cagcgccggg tcgtacactt gcagggtttt
     3001 cattttaaga ctccatccag cattgaaata atactgtcaa aaaatcacgc tcacgccgac
     3061 agctggccaa tgcggtccag ctcggctttc agcgcggcgc gttcgccgtg cagctgttcg
     3121 gccggcaagg ccagcagggc ttcgatgcgc gcgcgcagca cgcggtaggc gcgcacaaag
     3181 gcggcgtcga tgtcggccgg gctgccggtg gccttggccg ggtcgtccac cccccagtgg
     3241 ctgcgcaaca ccgggcccag atacgccggg caggtttcgc cggcggcgct ggcgcacacg
     3301 gtgatgacaa tatccggcgt caccggcagc gcgtcccagg atttgctggc gcaatcgctg
     3361 gcgtcgatgc cctcggcggc cagcagcgcc agcgagcgcg gatgcacttc gccagtgggc
     3421 tggctgccgg cgctgagtgc gcgccagccg gccggggcca ggtggttgaa ggtggcctca
     3481 gccaggatgg agcggcagga gttgccggtg cacaaaaaca gaatattcat tcaaggctca
     3541 caagcagggt cagaacacgt cgggtgggcg ccggccagcg gcggcaacag cgcctccggg
     3601 cagggcgagg cggcgcgcag gctggcgcat tgctcgggct ggccggcgca gcattcggcg
     3661 ctcaggtagg caatcagctc cagcatcagc gcaatattgg cgcgataacg ctgaaaccgc
     3721 ccttcctgcg tcaccgtcag caagccggcc tgggtcagcg ccttcaggtg aaacgacaga
     3781 ttggtgggcg gaatatccag ttcggtggca atggcgcccg ccaccaggcc atccagacca
     3841 tggcgcacca acaggcgata cacatccagg cgcacgccgg aggacagcga ttcaaacagt
     3901 ttggccgcag taaatttatc catgttttga ctatacaact aaatttgaaa tattgacaat
     3961 gaagatggtg tgacgcggcg cccacatggg cgccaagcgc gcctcaaccc agggtgacca
     4021 ccactggcgt gtggtctgat ggccgttcgt ttttgcgcgg ggcgcggtcg atctcgcagg
     4081 attgcgcgcg cgccagcagc ggcggcgaca gcaggatatg gtcgatgcgc aggcccaggt
     4141 tgcggcggaa catggccgcg cggtaatccc accagctgta ctggccgccg tcggcatgaa
     4201 aatgccgata gctgtcggcc aggcccatgg ccaccaggcg ctgaaacgcc gcgcgctcgg
     4261 gcgcggagca cagcaccttg ccggcccagc cggccgggtc gtacacgtcg cggtcgtccg
     4321 gggcgatgtt gaaatccccc agcaaggcca gctgcggcca gcgcgccagg ctgtcggcca
     4381 gatagcgctc cagcttgccc agccaggtga gtttgtaggc gtatttgtcc gaatccaccg
     4441 cctcgccatt gacgaaatac gcgcacacca cgcgcagctc gccaaagctg gcggcaatca
     4501 cccggcgttg cgggtcgtcg taatcgggga tgtctttgat gacctcagtg gcggtcagcg
     4561 tgtcgcggta gacaatcgcc acgccgttat aggttttttg cccggcaaat tcggcgcggt
     4621 agccggcggc ggccagggct tccagcggaa aggcgtggtc ttcttgcttg agctcttgca
     4681 ggcacagcac gtccaccgga ttggcggcca gccagtccag cacttgcggc aggcgcactt
     4741 taagagaatt gacgttccag gtggcgagtt gcatggcggt ttccttgagt cacaccgcct
     4801 tcaacgtgcg gctggcgcat tatgccagac gtttcggtgc ttgcggcgga cagggagcgg
     4861 cgcttgggcg ggctgaagga gaggcgtcag ccggggggcg caggacaaag gcgggggcgg
     4921 cttcgctgcc ggcgggggct ttggcggcag gttgcggtgc gcgcagaaac agcggaaacg
     4981 aggtgttgga taccggtttc atgacatctc ccgggtgcaa ggcggatcac atgattcagc
     5041 cagagtttgc cgtgtggcgt caagcctgcc acacggcatt catccggcat gggcggatgt
     5101 cagtgcataa gcgtgacatt ctgcccacac tgcgcattgc cgggggctgc cggcgggtgc
     5161 aagcgcaagc gacagggcac caccggagca tagccaatcc gtctacgggg gcgcatcaca
     5221 tgaatatgcg gggcgcaccg gttttggcgc gtattaacgc atttgcatca gtgtgcgggt
     5281 ggccacggcg gtgtcggtga agtcgctgaa cacgccgtcc acgcccagtt caaagaacag
     5341 ccggtattct tccgccgggt tgtcctggaa attggccgcc agcagcttgg cttcgttgcg
     5401 gaaggtgaac ggatgcacca ccaggcccag ctggtgggcg cgcttgacca cgtcgttggc
     5461 ctccagcagg gtcatgtcgc gctggtcgat cacattgtcg ccgttgacgt cgatgggctt
     5521 gccggttttc gggtcggtca tcgcctgcca gctgatcaga tacggcttcc acgggccgat
     5581 gccgtcggca aaggttttta cttgcttgag gttggccggg ctgagcatat cgccatagct
     5641 gcgcttgtcg ccgctgagca catggtcata cggcatcttg cgctcgacca cgccatccgg
     5701 gcgcacagtt tccgcatcca gcaggtacac cagcttgttg gaggtcagct tgcgcagttg
     5761 cttgaggttg gcggcttcaa aagactggat gaacaccggg gctttttgac tgttcagctg
     5821 atatttggcc agcagcgcca ccagcggttt ttccaggctc aggcccagtt gctggtgata
     5881 ggtcgggtgc ttggtttccg ggtagacgcc aatctggcgg ccggtttctt tggatttttg
     5941 cgcgcgcagg gcgaggattt cgtcaaaagt cggaatctga aactgcccgt caaacgcctt
     6001 gctgcggtcg gcgcgcggct ggatggcgcg cagggttttg atttccgcca gggtaaagtc
     6061 gctggcgaac cagccttctt cttccacgcc atccaccatc atcttgcgct tgcggctggc
     6121 gaattctgca cggcgggcca cgtcggtggt gtctttcaga ttgggttcgt ggcgggcgat
     6181 cagcacgccg tccttggtca tcaccagatc cggctcgata aagtccgcgc ccagctcgat
     6241 ggccttggcg tagccttcca gcgtgtggtc gggcagataa cccgaagcgc cacggtgggc
     6301 gatgatcagc ggcggctggc cattgagggt gggaaacact ggcttgccgg ccgccagcgc
     6361 gggcagggac agcgcggcgg cgctgnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6481 nnnnngcgcc aagggtggcg ggcgaacagt ctggcacagc aagatgaggg ttttttgacg
     6541 gcctccccga ccctaggccg ccgcaccacg cgcctggcca ggcgcacgat gcggcgtgtg
     6601 gtgagcggcg cttcaagtgg cgcttacggc aggtagggct tgcccagcga cgccggcagg
     6661 ttcagttgca acaagcggcg gtcgcgcgag atcagcacca gcaccacaat ggtggccagg
     6721 tagggcagcg ccgccagcag ttgcaccggc agggcaatgc ccaggccctg ggcgtggaat
     6781 tgcagaatgg tcaccgcgcc aaacaaatac gcccccagca gcaggcggcc cggcttccag
     6841 gcggcaaaca ccaccagcgc cacggcaatc cagccgcgcc cggcgctcat gttctgcacc
     6901 cacagcgggg tgtagacagt ggacagatag gccccggcca gcccggccat tgccccgcca
     6961 aacagcaccg cgccatagcg gatggccagc accggatagc caatggcgtg cgccgcctcg
     7021 ggcgattcgc ccaccgcttt cagcagcagg cccagccggg ttttcgccaa ggtccacgcc
     7081 accagggcaa acgccgccag cgacagatac accacggcgt cttggtgaaa cagcaccggg
     7141 ccaatcaccg ggatatccga caacagcgga atcgacagcg gcttgagccc cggcaggctg
     7201 tagctgacat agtgctggcc aataaacgcc gacaggccaa tgccaaaaat ggtcagcgcc
     7261 agcccacagg cggcctggtt ggcggccagc gacagcgcaa acacggcaaa caccagcgcc
     7321 gcgcccatgc cggccagcgc gccggccggc aggccagcgg ccgggcccag cccggtttcg
     7381 gcggcggcgg caaagccggc aatcgcgcca atcaaaatca tgccttccac gccaaggttg
     7441 atcaccccgc tgcgctcggc caccagctcg cccagcccgg caaaaatcag cggcgtggcc
     7501 gccgccagcg tgccggccag caccggaatc atggaatcca tcatgcttgc atctcctggc
     7561 gcgccgcctc cagccagcgg cgcgcgtctg tgtccggcnn nnnnnnnnnn nnnnnnnnnn
     7621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7681 nnnnnnnnnn nnnnnnnntg cttgcatctc ctggcgcgcc gcctccagcc agcggcgcgc
     7741 gtctgtgtcc ggccgctgcg cgcagccggg taaaacggtt ttgggttcag tcggtggcca
     7801 tgccacgggt ggcgcgccgt gcttacttgc gccgacgcag gcggttgtcg ataaaggcat
     7861 cggcgccgag caggaagaac agcagcatgc cctgaaaaat cccggtaatc gccgccggca
     7921 gttgcagcgc cacttgtgcg gcttcgccgc ccaggtagat cagcgccatc agcgcgccgg
     7981 ccagcacgat gcccagcgcg tgcaggcggc ccagataagc gacgatgatg gcggcaaagc
     8041 cgtagccggg cgagatgttc aggttaagct ggcccaccgg gccggccact tcgcccacgc
     8101 cggccaggcc ggcggtgagg ccggagagca ccaggctgaa ccacaccgtg cgctgggcag
     8161 aaaacccggc gtagcgcgcc gccgccgggg cctggccgcc cacttccagc tgataggcca
     8221 aaaagctctt ggcgttgaac aaccagaagc ccagcacggc aaacagggtg atgaacaccg
     8281 agccattcac ccgcgagcct tccagcagga tggggaacag cgcgccatcg gcaaacatca
     8341 ccgattgcgg aaaattcatc ccctccgggt cgcgccacgg gccgttgacc atatacgaca
     8401 gcagaaaact ggccacataa gccagcatca gcgtggtgag ggtttcttcg gcgttgaagc
     8461 gggttttcag tagcgcagga atcgcccccc acagcgcgcc gcccagtgcg ccggccagca
     8521 ccatggccgg cagcacccag gccgcgccgc tgtcgccaaa ctggatggcg atgccgccgc
     8581 cggccagcgc gccgacaatc agctggcctt cggcgccgat attgtaaatg cgcgccttaa
     8641 agccaatggc caggccctgg gcgatcagga tcagcggaca ggccttgatc agcagttcgc
     8701 tccagccgta gctgctggtc agcggctcga caaaaaacac cttgaacgat tgcagcgggt
     8761 ctttgcccag cgccagaaac agccctgacc ccaccagcag ggtggcgaca atggcgatca
     8821 gcggcgcggt ccagcgcatc agttgcgagg gttgcgggcg gggttcaagc agatgcatgg
     8881 ctggcctccg cgttgaacag gccggccatt tgcaggccca ccgactcggc gtcggcctcg
     8941 gccttgggca cggccgccga cagccggccc tgggccagca cggcgatgcg gtcgctgatt
     9001 tcaaacaatt cttccaactc ctcggaaatc accagaatcg ccacgcccga gcgggacaaa
     9061 tccagcaggg tctggcgcaa aaacgccgcc gcgccgacgt ccacccccca ggtgggctgg
     9121 gcgacgatca tggctttcgg gctgagcatg atctcgcgcc cgacaatgaa tttttgcaaa
     9181 ttgccgccgg acagcgaacg cgccgccgcc agcgggccgc cgcagcgcac gtcaaagcgg
     9241 gcgatgcagt cctgggcaaa ctggcgcgcg cgcgcatagc tgaccaggcc aaaacggcgc
     9301 atgccgccgt ggcggtggcc ggtgagcagg gcgttatgcg ccagcgattg cgagggcacc
     9361 gcgccgcgcc ccaggcgctc ttccggcaca aaggccagcc cgcgcgcgcg tcggcggccg
     9421 gggtgcaaat ggccaattgc ttcgccatcc agccagacgg tggtcttgcc gctgggcgct
     9481 tcgcccgaca gcgccgccag cagctcggcc tggccattgc cggaaatccc ggcaatgccg
     9541 acgatctccc ccgcgttcac cgtcagcgag acatcactta gcgacgtgcc aaacgggtcg
     9601 tcgctgcgct ggctgagctg gcggatttcc agcaccggcc ggctggggcc acggtagggc
     9661 ggcgcttcgc acaccggcag ttcgcggccg atcatcatcc gcgccaggct ggccggggtt
     9721 tcatcacgcg gaatcgcctc gccggtcacc ttgccgccgc gcagaatggt ggcgctgtcg
     9781 cacagggttt ggatttcgtg cagcttgtgg ctgatgtaga caatcgacac gccctcgctg
     9841 gccagccggc gcagggtgtc gaacagcttt tgcaccgcct gcggggtgag caccgaggtg
     9901 ggttcgtcca ggatcaacag cttggggtct tgcagcaggc agcgcacgat ttccacccgc
     9961 tggcgctcgc ccaccgacag gctgtgcacc agccggtgcg ggtccagcgg caggccgtag
    10021 cgctcggaca cctcgcgcac ccgcagcgcc agcgccgccg ggtcgaacgg cgcatccagc
    10081 gccagggcga tgttctcgac cacggtcagg gtttcgaaca gcgaaaaatg ctgaaacacc
    10141 atgccaatgc ccagcttgcg cgcctgcgcc gggttggcga tggtcaccgg ctggccgttc
    10201 cagcggattt cgccagcgtc ggccttgacc gcgccataga tgattttcat cagcgtgctt
    10261 ttgcccgcgc cattttcgcc aagaatggcg tggatctcgc cgggcgccac cgacaggctg
    10321 acgccatcat tggcgaccac gctggggtaa cgcttgctga tcccggtcag ggtcagacgg
    10381 ggaggggtct gcgacatgct tactccggat aaaaaacagt cggctgaatc agcgtatgcg
    10441 cctgattcag ccattggcgt gcattgtgca gctgcaacat ctgcgcggca acggccaagg
    10501 caatcgccgc cggctgtttg tcggtgatgc cggcaatgcc aatcgggcaa gtcagctcgc
    10561 tcaggcgggc ggcgtccagc ccgcgcgcca gcagccgctg ctcaaaactg gcgcgtttgc
    10621 tgtgcgagcc aatcaggcca aaatacgccg cgtcgcggcg ggcaaagatg gcttcgcaca
    10681 gggcaaaatc cagcgcgtgg ctgtggctca tcaccagata aaaacagccc ggcggcgcct
    10741 cggccacttc gctggccggg tcgtcgttca cccgcgcgtc caccccggca agatccagcc
    10801 cggcaaaggc gtcgtcgcgc gggtcgatcc agcgcacctg cgccgccagc aggcgcagtt
    10861 gcgtcaccag cgcctgcgcc acatggccgg cgccaaacag ccacaccgga aacggcgcgc
    10921 gcgccagccg ctgccgccag cgccagtctt gctcgccgcc gtccagcgcg ctgctggcct
    10981 cggtgggcgc ggccaagcgc cagcgtgagg ccgcttcgcc agcgttcagt tcacgccaca
    11041 gcgcctggcc ggcgctcagc tgggcggcgc gcgcctgcca gtcggccagc gcgctggggg
    11101 caatcacctc aaacagcagc cacaccacgc cgccgcagca ctggcccagc cgcgcgccca
    11161 gattgtagcg gcagacgcgg gccgccgggt ggccagtgct ggcgctgagc agctggcgcg
    11221 cctgggcgat ggcctgccat tccagatggc cgccgccaat ggtgtcggcc tgacggtgcg
    11281 cgcccaccac catgctggcg cccacttcgc gcgggccgga gccatgcagc cgcgccacgc
    11341 tcacccacac catcggttcg cccgccgcca ggccatccag cagcgcgttc agccagcgca
    11401 tgcgccgctc ccggcgcgca gcgcgcgcac aatcgcctct ggcgtggccg gcgcgtccag
    11461 ttgcaccggc gcgccggcgg gcagggcggc ggccaccgcg tcgcgcaggg caaaaaacac
    11521 cgccagcgcc agcatgaacg gcggctcgcc caccgccttg ctgcggtgga tgctgtcttc
    11581 gcggttggcg ttggcgtaca gcgccacatt gaacaccgcc ggcacatccg ctgcggtggg
    11641 gattttgtag gtggacggcg cgtgggtcat cagcttgccg tcggcgcgcc agtgcagttc
    11701 ttcgctggtc agccagccca tgccctgcac aaagccgccc tcgatctggc cgatgtccag
    11761 cgccgggttg atcgactggc cgacgtcgtg cagcaggtcg gcgcgcagca cccggctttc
    11821 gccggtcagt gtgtcgatgg ccacttcgca ggccgccgcg ccgtaggcaa agtaataaaa
    11881 cggccggccc agcttgctga ccgggtcaaa gtgaatgtcc ggggtgcgat aaaacccgct
    11941 gtcccacagc tgcacccgcg cccggtaggc gcgcatggcc agctcggcaa acggcaggct
    12001 gaagtccgcc agccgcacct ggccatcggc aaaccgcacc gcgtcggcgc tgccgccgcc
    12061 cgccagctcg accatcagcg ccgccagccg cgccttgatc tgctggcagg ccgcctgcgc
    12121 cgccttgccg ttcaggtcgg ttccgctgga ggcggcggtg gccgaggtgt tggacacccg
    12181 gctggtgtcg gtggcggcca gccgcacatc ggccagcggc aggccaaatt caccggcgac
    12241 gatctggcga atcttggtgt acaggccctg gcccatctcg gtgccgccgt gcgaggccag
    12301 cacggtgccg tcggtgtaga catgcaccag cgcgccggcc tggttgaagt gggtggcgtt
    12361 gaacgagatg ccaaatttca ccggggtcag cgccaggccg cgcttgagcg tggggctggc
    12421 ggcgttgaat tcggcaatcg cctggcggcg ctgggcgtat tggcagcgcg cggccagctc
    12481 ggccaccagc gccggggcga tattgtcggt gaccggcatg ccgtagtggg tgacgctgcg
    12541 cgcgccgccc ggctcgctgt cgtaaaaatt ggcctgacgc accgccagcg ggtcgagctg
    12601 caaatggcgg gcgatgtcgt ccagcacggt ttcgatcaag aacatgccct gcgggccgcc
    12661 aaaaccacga aacgcggtgt tggatacggt gtgggttttg cagcgcaggc tgcgcagcgc
    12721 cagcgcatcc agataatagg cattgtccag atggcagatg gcgcggtcgt tgaccgggcc
    12781 agacaaatcc gccgaaaacc cgcagtgcga ggccatctcc agcttgagcc cggccagccg
    12841 gccatcgtcg gcaaacgccg cctgccattg atactgaaag ccatggcgct tgccggtggc
    12901 gctcatgtcc tcgtcgcggt ccagccgcag cttgactggc cggccagtgc gcaccgccag
    12961 cagcgccgcc aggcaggccc attgcgccga ctgggattct ttgccgccaa acgccccgcc
    13021 catgcgccga caactgacgg tgatttgatg cgcgcgccag cccagcgcgt gggcaatcag
    13081 ctgctgcatc tcgctggggt gttgggtgga gcaccacaca tgcaggctgt cgtcgtcgcc
    13141 gggctgggcg taagaaatct gcccttccag ataaaaatgc tcctgcccgc ccacccgcgc
    13201 ctggccagac aagcggtgcg gcgcggcggc aaaggcggcg tccacctcgc cgcgctgcat
    13261 ctcgaacggc ggcagcaccc aggattcggc ggccaccgcg ctgtcgatgt ccagcagcgc
    13321 cggcaagggc tcgatgtcga tctgcgccag ccgcaccgcc tggcgcgcct gctcgcggct
    13381 gtcggcggcc accacaaaca gcggctggcc gtaaaactgc acctcatccg ccgccagcag
    13441 cgggtcgtcg tgcaggatgg ggccgcaatt attgtcgccg ggaatatcca ctgcggtgag
    13501 cacggcgcgc acgccggggg cggcgcgcac cgcgtccagc tgcaaggcgc gcaggcggcc
    13561 atgcgcacag ggggcctggc caatggcggc gtgcagggtg ccggccagtt cgggcaggtc
    13621 gtccagatag tgggcggcgc cgctgacgtg cagcgcggcg ctttcgtgcg gcagggcatg
    13681 gcctaccggc ttgggtgggg tgtcggcgcg catctcagca gccctccaga ctaatcggag
    13741 ccagggcgtc cagccgatgg ccggcctggc cctggctgtc cagccagaag cgccacagca
    13801 gttgcgcggc gacatggcgg cggtagtcgg cgctggcgcg ggcgtcgctc agcggctgaa
    13861 aatcaccttc cagcgcggcc atggcggcgc gcacgctggc ggcgctccac ggcgcgccca
    13921 gcaaggcggc ctcggcctgc gcggcgcggc gcggggtggc ggccatgccg ccgtaggcca
    13981 gccgcgccaa gctcacttgg ccggcggcgt tggtgtgtac ctgcatgccg gcggccacgg
    14041 cggaaatatc ctggtcgtgg cgcttggcca ctttataggc gcggaattca cccagcgggc
    14101 gcggcacgcg cacggcgcgg acaaactcgc cctcggtgag cgcggtgcgc tggtaggcca
    14161 gataaaaatc ttccagcgcc agctcgcggc acacgtcgcc gcgttgcagg cgcagcgtgg
    14221 cgcccagggc aatcagcgcc ggcatgctgt cgccaatcgg cgagccattg gcgatattgc
    14281 cgcccagcgt gccggcgtgc ttgaccgggg tggaggcaaa gcggcgcgcc agctcggccc
    14341 agtgcggcgt ccactggctg agcgcggcaa aggcgtcgtg cagcggcgcg gcggcgccga
    14401 tttccagctg gccgtctact tcggcaatct gtttcagctc ggtcaccgcg cccagctgaa
    14461 tcaagctgtc cagcgcgcgg ccttgcttgg tcacccacag ccccacgtcg gtgccgccgg
    14521 ccaccagccg cgcggcgggg tgttgctggc gcagcgcggc cagctcggcc aggctgcgcg
    14581 ggctgaaact ctcgccgccg tcagcggcgc gatagtgcaa aggcggcagc gccgccagct
    14641 ggcgcaaggc ggctgccacc ggcgcgctgt cccactcccg gcgcggggcc tgccaggcgg
    14701 cctgggcggc gtcgagaatt ggccggtagc cggtgcagcg gcacagattg cccgacagac
    14761 agtcttccag ctcggcgcgg ctggggcagt cgggctggct gtggtagcgg gcaaacagcg
    14821 acatggcaaa gcccggcgtg caaaagccgc actgcgcgcc gtggtggtcg atcagcgcct
    14881 gttgcagcgg gtgcaggcca tcggcgtcgg ccaggtcttc cacggtgaac agcgccttgc
    14941 cgtccagcgc cggcaaaaac tgaatacagg cgttcaccgc gcgcaggcgc aggccgccgt
    15001 cggcgtccag ctcgcccagc accacggtgc aggcgccgca atcgccctcg gcgcagcctt
    15061 ccttgctgcc ggtctggcgc aggtcttcac gcaggtattg cagcacggtg cgggtgggcg
    15121 aggggccggc gatctggcgc aggctgccgc gaaaataaaa ctgaatcggt cgttcgatca
    15181 tgatgcatac actcaagctg tcatgcgctc aaagtagcac atggcgcgcg ccggcaggca
    15241 ggggccggca gcgataagca ctatgcgccg cagattatgg ctggcgactg gccgcgcgtg
    15301 catcctgcca cgctggccgg gaatgactgt tgtatgattg ccggattccc ccactccctg
    15361 cggaatccaa gccatgaatc ttgtcaccta tctgggcaag caaatccttg gcgtgctggc
    15421 ctgttaccaa caggacgaca tcgtcattca catctgcggc gtataccctc aatgggataa
    15481 ccgcctgcgc gtgttttttc cgcgcggcca tgccttgcag gtgggcgaac tggttaccct
    15541 gcatctggat aaccgcagcg gcgtggatga
//
LOCUS       DAOPYX010000028        15514 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_4089_length_15514_cov_13.614982, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000028 DAOPYX010000000
VERSION     DAOPYX010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 15514)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15514)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15514
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4089_length_15514_cov_13.614982"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(2..748)
                     /locus_tag="PKN55_02670"
     CDS             complement(2..748)
                     /locus_tag="PKN55_02670"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011134215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acyltransferase"
                     /protein_id="HNX70180.1"
                     /translation="MLQERLARAPRAARLSVRLAANADELRQAQKLRYRIFGEEMGAQ
                     LASAQLGIDCDEYDELCDHLVVVSPDGEVVGTYRMLSPDNARRAPKLYSEHEFDLSRL
                     THVRDSLVEVGRSCVHADYRTGGTIALLWSGLAQYVREHNAEYLAGCASVSLNDGGHQ
                     AASLYRKLEASHLAPAEWRVFPHLPLPLAQLTDTVDVAIPALIKGYLRAGAFVCGEPA
                     WDPDFNCADFFMLLPMGRVTERYNRHFVGE"
     gene            939..2840
                     /gene="mnmG"
                     /locus_tag="PKN55_02675"
     CDS             939..2840
                     /gene="mnmG"
                     /locus_tag="PKN55_02675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014088604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HNX70181.1"
                     /translation="MIYPTQFDVIVVGGGHAGTEAALAAARMGRATLLLSHNIETLGQ
                     MSCNPSIGGIGKGHLVREVDALGGAMALATDMGGIQFRTLNSSKGPAVRATRAQADRV
                     RYKAAIRHMLENQDNLMLFQQPVDDLLLDGDRVAGVITQAGITFRARSVVLTAGTFLS
                     GKIHIGLENYTGGRAGDPAALTLGARLRELALPVGRLKTGTPPRLDGRTIRFDLLEEQ
                     PGDDPRPVFSVRGSRDMHPRQLPCWITHTNTQTHDIIRSGFDRSPMFTGVIEGVGPRY
                     CPSIEDKINRFADRDSHQIFLEPEGLDTHEIYPNGISTSLPFDIQLAAVRSMRGLENA
                     TILRPGYAIEYDYFDPRALKSTLESKHIAGLFFAGQINGTTGYEEAAAQGCLAGINAG
                     LYVQERDGWSPRRDQAYLGVLVDDLITRGVTEPYRMFTSRAEFRLQLREDNADLRLTE
                     AGRALGVVGDAQWAMFCRKRDAVAAELERLRSTWVNPRVLAAADAERVLGKAIEREYN
                     LQDLLRRPQVSYASLATLPGFGAEPAPDEVAEQVEIQVKYQGYIERQAEENARRDQIE
                     HIALPADLDYASVPGLSKEVQQKLGQHRPETLGQASRLSGMTPAAVSLLLVYLKRAGN
                     DARKTSHDA"
     gene            2830..3450
                     /gene="rsmG"
                     /locus_tag="PKN55_02680"
     CDS             2830..3450
                     /gene="rsmG"
                     /locus_tag="PKN55_02680"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003767114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HNX70182.1"
                     /translation="MTLEQELEAGLAQLGLELSAEQIDRLNQYLALLNKWNKTYNLTA
                     VRETERMVAYHVLDSLSALPHIQGVRVLDVGSGGGMPGMLFAIARPDWQLTLIDANHK
                     KTTFLRQAAIELGLNNVEVHCERVEALAAPAFDVITSRAFADLAEFVRLTRHVLADGG
                     VWAALKGVYPYEEIAQLPDDIRVVSVQALHVPGLDAERHLVTLARV"
     gene            3469..4260
                     /locus_tag="PKN55_02685"
     CDS             3469..4260
                     /locus_tag="PKN55_02685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007341697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HNX70183.1"
                     /translation="MATVIAITNQKGGVGKTTTVVNLAAGFAAKGLRVLLVDLDPQAN
                     ATMGCGIDKAKLPAGGLYRLLLGEATLEAVRCRSESGGFDVLPANRELGGAEVELVSA
                     PRREQRLKDALAEVRADYDLILLDCPPALNLLTLNGLAAASRVIIPMQCEYYALEGLS
                     DLVNTLKKARASLNPGIEMLGLLRTMYDPRFTLSQQVSAQLQQYFPGKLFETAIPRNV
                     RLAEAPSHGMPALAYDRASRGAKAYLALVEEIAQRLHLTLPKQVG"
     gene            4264..5106
                     /locus_tag="PKN55_02690"
     CDS             4264..5106
                     /locus_tag="PKN55_02690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008952155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HNX70184.1"
                     /translation="MAKPKGLGRGLDALLAVNHSDDQLQTLPIHAIEPGKYQPRTHMD
                     AAALEALAESIRAQGVIQPLVVRPVGYERYELIAGERRWRASQLAGLAEVPAVVRTVP
                     DEAALAMALIENIQREDLNPLEEALGLKRLADEFGMTHEAVAAAVGRSRSAVSNLLRL
                     LSLAEPVQALVNDGKLDMGHARALLTLPVLQQVSLAQDIAERGLSVRDAEQRAQQLLH
                     PPAPRSEARADPDVLRLAEEVSEKVGAKVSIRPGRKGSGKLVVEYATLDQLDNLIAAL
                     SGRQ"
     gene            5187..5585
                     /locus_tag="PKN55_02695"
     CDS             5187..5585
                     /locus_tag="PKN55_02695"
                     /inference="COORDINATES: protein motif:HMM:NF015834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HNX70185.1"
                     /translation="MSIISEFVLGLPKQLADAVSKLLRWQIRLSLPVVIVSGILGMPS
                     WHAPVSALLGALVGIVPALVYVRIAYRKPRGAPGKLLSAHFAAEAAKLAVTGLMFALV
                     LALYKDVVPLALFSSFFATLVAYWIALLSK"
     gene            5600..6388
                     /gene="atpB"
                     /locus_tag="PKN55_02700"
     CDS             5600..6388
                     /gene="atpB"
                     /locus_tag="PKN55_02700"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014088599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HNX70186.1"
                     /translation="MAGSATDYIKHHLTHAKAPHDGGFWSLNVDTFSVSLILGFIFLF
                     VFARVVRNFSVENPGRLQCLVESIVEMVGNQVRDIFHAKSDMVAPLALTVFCWVFLMN
                     AMDMLPVDLLPYIAQLIGGAMGADPHHVYLRVVPSADVNATFAMSLSVMFLIIGFSIK
                     AKGFGGYMHELFSAPFGPKVGPINFIFQLIELIAKPISLALRLFGNMYAGELIFILIA
                     LLPWYVQWLLGGPWAIFHILVITLQAFVFMMLTIVYLSLAVEDH"
     gene            6456..6749
                     /gene="atpE"
                     /locus_tag="PKN55_02705"
     CDS             6456..6749
                     /gene="atpE"
                     /locus_tag="PKN55_02705"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008952158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HNX70187.1"
                     /translation="MEAFVSQIQGMTALAAAIMIGLGAIGTALGFGILGGKFLESSAR
                     QPEMIPVLQTKLFIIAGLLDAISMIGVGVGLLYTFNNPFLTAGVAALKAAAGH"
     gene            6796..7266
                     /locus_tag="PKN55_02710"
     CDS             6796..7266
                     /locus_tag="PKN55_02710"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HNX70188.1"
                     /translation="MDINATLLGQALTFAVLVWFTMKFVWPPLTNMMDERAKRIADGL
                     AAAERGKQDLEEASRRVEAEIRAAQQKATEMLINAEKRANQIVEEAKQAARTEGERII
                     ADAKAEIDQEVLRAKESLRSQVAGLAVAGAEKILRKEVDAAKHADLLASIKAEL"
     gene            7274..7810
                     /locus_tag="PKN55_02715"
     CDS             7274..7810
                     /locus_tag="PKN55_02715"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014088597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HNX70189.1"
                     /translation="MAEIITVARPYAEAVFRLAKDANRLAPWGEALAVLAAMMEHSEV
                     VTLVTNPKSTAQEVGKLIVDVLGDRADVEVKNFIDALQENHRLTLLPEIARQFELLKS
                     REESVVEATVESAFPLNDQQAQELAGTLKAKFGKDVRLSTSVDPDLIGGVRIKVGDDV
                     IDASVRGKLQAMAVSLKS"
     gene            7819..9363
                     /gene="atpA"
                     /locus_tag="PKN55_02720"
     CDS             7819..9363
                     /gene="atpA"
                     /locus_tag="PKN55_02720"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008952161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HNX70190.1"
                     /translation="MQLNPSEISELIRAKIQNLPSSTEVRTTGTVVSVTDGIVRVHGL
                     ADVMQGEMLEFPGNTFGLAMNLERDSVGAVVLGEYEHISEGDEVKTTGRILEVPVGRE
                     LVGRVVNALGQPIDGKGPLNTKLSSPIEKIAPGVIARKSVDQPMQTGLKAIDSMVPIG
                     RGQRELIIGDRQTGKTAVALDAIINQKGTGVVCIYVAVGQKASSIANVVRKLEEHDAL
                     GHTIIVAASASEAAALQYIAPYSGCTMGEYFRDRGEDALIVYDDLSKQAVAYRQISLL
                     LRRPPGREAYPGDVFYLHSRLLERAARVNADYVEKFTNGEVKGQTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLESDLFNSGIRPAINAGISVSRVGGAAQTKVIKKLGGG
                     IRLALAQYRELASFAQFASDLDEATRKQLQHGEIVTELMKQKQFSTLKVSEMALTLFA
                     VTKGFYEGVPVKKALAFEAAFLSHMKANQAAVLSAIEASGELSGDNEKILAKELESFK
                     AGFNFN"
     gene            9397..10263
                     /gene="atpG"
                     /locus_tag="PKN55_02725"
     CDS             9397..10263
                     /gene="atpG"
                     /locus_tag="PKN55_02725"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003705028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HNX70191.1"
                     /translation="MAVGKEIRTKIKSVQNTQKITRAMQMVATSKMRKTQDRMRAARP
                     YAEKVRQVMAHLAQTNPDANNPLLAKRSSVKRAGVILITSDKGLAGGLNANLLRRFCQ
                     RVDELMGQGVQVDVCALGQKGLAAAQRLKLNVVASATQLGDTPQMDKLIGPISVMINA
                     YAEGTIDELHIVYTRFVNTMKQEPTLEQLLPLSTKHMAVEHSHPWDYLFEPDVKSVLD
                     FLVRRYLESVIYQGVAENMASEQAARMVAMKAATDNAANLIESLKLTYNKARQAAITT
                     ELSEIVAGAAAV"
     gene            10300..11676
                     /gene="atpD"
                     /locus_tag="PKN55_02730"
     CDS             10300..11676
                     /gene="atpD"
                     /locus_tag="PKN55_02730"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008952163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HNX70192.1"
                     /translation="MSQGKIVQIIGAVIDVEFPRDAMPKVYDALKVLETGLTLEVQQQ
                     LGDGVVRAIAMGSSDGLRRGLAVANSGAPVSVPVGKATLGRIMDVLGDPIDEKGPIGE
                     EKRMPIHRKAPAFDELSAATELLETGIKVIDLLCPFAKGGKVGLFGGAGVGKTVNMME
                     LIRNIAIEHSGYSVFTGVGERTREGNDFYHEMNDSNVLDKVSLVYGQMNEPPGNRLRV
                     ALSGLTMAENFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEMGK
                     FQERVASTKAGSITSIQAVYVPADDLTDPSPATTFAHLDATVVLSRDIAALGIYPAVD
                     PLDSTSRQLDPLVVGEEHYNVARGVQMTLQKYKELRDIIAILGMDELSEEDKLTVARA
                     RKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIAGFKAILSGEYDHLPEQAFYMVGGIDE
                     VIEKAKTV"
     gene            11704..12117
                     /locus_tag="PKN55_02735"
     CDS             11704..12117
                     /locus_tag="PKN55_02735"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009426372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HNX70193.1"
                     /translation="MHVDVVSAEAQIYSGEAEFLVAPAEMGEIGVYPRHVPLLTRIKP
                     GSLRIRVPGQAKEVLVVVSGGMMEVQPSLITVLADTAIRGEELDEQRAQAAKRDAEAA
                     LSKATDDQETAQARAALKAAIAELHALEYLRKRVH"
     gene            12270..13640
                     /gene="glmU"
                     /locus_tag="PKN55_02740"
     CDS             12270..13640
                     /gene="glmU"
                     /locus_tag="PKN55_02740"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011134229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HNX70194.1"
                     /translation="MNPVSVVILAAGRGKRMYSDLPKVLHPIAGKPMLARVIDLALSL
                     LPSQCVVVIGHGAEQVSAAFAGHAGPLAFALQSEQLGTGHAVKMALPHLPSEGRTLVL
                     YGDVPLTEAATLRRLLDTPADDVALLTDTLVNPTGYGRIVRDAAGRIQCIVEEKDASA
                     EQKALCEINTGIVALPNARLAGWLAELKNGNAQGEYYLTDVIGLAVRDGVTVHGVGAP
                     ASWQVAGVNNKAQLAELERIFQANQARVLLDAGVTLADPARIDIRGELRCGRDVSIDV
                     NCVFEGDVTLGDGVSIGPNCVLKNVSLAAGVQVAAFSHLDGAQVGADARIGPFARLRP
                     GARLAEDVHIGNFVEIKNSALSAGAKVNHLSYVGDADVGAKVNVGAGTITCNYDGVNK
                     FRTTIEAGAFIGSGTMLVAPVTVGAGATIGAGSVISKDAPAEQLTVARAKQLSLSGWQ
                     RPLKKT"
     gene            13743..>15514
                     /gene="glmS"
                     /locus_tag="PKN55_02745"
     CDS             13743..>15514
                     /gene="glmS"
                     /locus_tag="PKN55_02745"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HNX70195.1"
                     /translation="MCGVVGAIAQRNVVPILLEGLRRLEYRGYDSVGVAVVGEQGLAR
                     ERSTERVAELAEQTRRVGLKGLTGIGHTRWATHGAPCERNAHPHFSHGLLSLVHNGII
                     ENHQPLRERLAAQGYAFESDTDTEVMVHLIHSYYVTHHDLLRAVQQTLAELHGAYAIG
                     VIAADNPDVMVCARHGSPLLLGLGIEENFFASDVSALLPVTQKVIYLEEGEVARLTLT
                     EVQVFNQAGERVEPVVHVSEQSADVTELGEYRHYMQKEIHEQPRALADTLERVGNTIE
                     PSLFGAEAPALFADINAVTIIACGTSYHAGLVAKYWLESLAGLPTSVEVASEYRYRDS
                     VANPSALVVTISQSGETADTLAALKHAKALGHDKTLSICNVPESSLIRLSALRFLTHA
                     GPEIGVASTKAFTTQLVALYLLTLALAKVRGRLSAEREADALADLKRLPGAVNALLAL
                     ESGLEQWAEALATRQHALFLGRHTLFPIALEGALKLKEISYIHAEAYPAGELKHGPLA
                     LVDRDMPIIVCAPNDALFDKLASNMQEVRARNGELFVLSDAAVEAGPGQHVLALPAGM
                     RDLNPLLYTIPLQLLAYHTAIRKGT"
BASE COUNT         2967 a         4821 c         4856 g         2870 t
ORIGIN      
        1 cttattcgcc gacaaagtgg cggttgtaac gctcagtcac ccgccccatc ggcagcagca
       61 taaagaaatc cgcgcagttg aaatccgggt cccaggccgg ctcgccgcag acaaaggccc
      121 cggcgcgcag ataacccttg atcagcgccg gaatcgccac gtccacggtg tcggtcagct
      181 gggccagcgg cagcggcagg tgcgggaaca cccgccattc cgctggcgcc aggtggctgg
      241 cttccagttt gcggtacagg ctggccgcct gatggccgcc atcgttcagg ctgacgctgg
      301 cgcagccggc caggtattcg gcgttgtgct cgcgcacata ctgcgccagg cccgaccaca
      361 gcagggcgat ggtgccgccg gtgcggtagt ccgcatgcac gcaggagcgg ccgacttcca
      421 ccaggctgtc gcgcacatgg gtgaggcggg aaagatcgaa ctcatgttcg gaatacagct
      481 tgggcgcccg gcgggcgttg tccgggctga gcatgcgata ggtgccgacc acttcgccat
      541 ccgggctgac caccaccagg tgatcgcaca gctcgtcgta ctcgtcgcag tcgatgccca
      601 gctgggccga ggccagttgc gcgcccattt cttcgccaaa aatgcggtag cgcagttttt
      661 gcgcctggcg cagttcgtcg gcgttggcgg ccaggcgcac gctcaggcgg gccgcgcgcg
      721 gggcgcgggc aagtctttct tgcagcatgg ttttctcccg atcatggttg tccgccagtg
      781 tgcgagccgg cgatgacaag cgtttgacgc cgaggtgacg gaatcatgat cgggtaaaaa
      841 cccggccggc ggctggcaaa tcaacaactt acgccgagaa cggttttcgc atacaatact
      901 gccccctgtt caagcacttt cccgctggtg agcgcgccat gatctacccc acccagtttg
      961 acgtgattgt tgtcggcggc ggccacgccg gcaccgaagc tgccctggct gccgcccgca
     1021 tgggccgcgc cacgcttttg ctcagccaca atatcgaaac cttgggacag atgtcgtgca
     1081 acccgtcgat tggcggcatc ggcaaaggcc atctggtgcg cgaagtggac gcgctgggcg
     1141 gggcgatggc gctggccacc gatatgggcg ggattcagtt tcgcacgctg aacagctcaa
     1201 aaggcccggc ggtgcgcgcc acccgcgccc aggccgaccg cgtgcgctac aaggcggcca
     1261 tccgccatat gctggaaaac caggataatc tgatgctgtt ccagcagccg gtggacgatt
     1321 tgctgctgga cggcgaccgc gtggccgggg tgatcaccca ggctggcatc acttttcgcg
     1381 cccgctcggt ggtgctgacc gccggcacgt ttttgtccgg caagattcat atcggcctgg
     1441 aaaactacac cggcgggcgc gctggcgacc cggcggcgtt gaccctgggc gcgcgcctgc
     1501 gtgagctggc cttgccggtg gggcggctga aaaccggcac gccgccgcgc ctggacgggc
     1561 gcaccatccg ctttgacctg ctggaagaac aacccggcga cgatccgcgc ccggtgtttt
     1621 ccgtgcgcgg ctcgcgcgac atgcacccgc gccaactgcc ctgctggatc acccacacca
     1681 atacccagac ccacgacatc atccgctcgg gctttgatcg ctcgccgatg ttcaccgggg
     1741 tgatcgaagg cgtggggccg cgctactgcc cgtcgattga agacaagatc aaccgctttg
     1801 ccgaccgtga cagtcaccag atctttctcg agccggaagg gctggacacc cacgaaatct
     1861 acccgaacgg gatttccacc agcctgccgt ttgatatcca gctggcggcg gtgcgcagca
     1921 tgcgcgggct ggagaacgcc accatcctgc gtccgggcta cgccatcgaa tacgattatt
     1981 ttgatccgcg cgcgctgaaa tccacgctgg aaagcaaaca catcgccggt ttgttcttcg
     2041 ccggccagat caacggcacc accggctacg aagaagccgc cgcacaaggc tgcctggctg
     2101 gcatcaacgc cgggctgtat gtgcaagagc gcgatggctg gagcccgcgc cgcgaccagg
     2161 cctatctggg cgtgctggtg gatgatttga tcacccgtgg ggtgaccgag ccgtaccgca
     2221 tgttcaccag ccgcgccgag ttccgcctgc aattgcgcga agacaacgcc gacctgcgcc
     2281 tgaccgaggc tggccgcgcg ctgggcgtgg tgggcgatgc gcagtgggcg atgttctgcc
     2341 gcaagcgcga cgcggtggcc gccgagctgg agcggctgcg cagcacctgg gtgaatccgc
     2401 gcgtgctggc cgccgccgac gccgagcgcg tgctgggcaa ggccattgag cgcgaataca
     2461 acctgcaaga cctgctgcgc cgcccgcagg tcagctacgc cagcctggcc accctgcccg
     2521 gctttggcgc cgagccggcg ccggacgagg ttgccgagca ggtggaaatt caggtcaaat
     2581 accaagggta tattgagcgc caggccgaag aaaacgcccg ccgcgaccag atcgaacaca
     2641 ttgccttgcc ggccgatctg gactacgcca gcgtgcctgg gctctccaag gaagtacaac
     2701 aaaaacttgg ccagcatcgc ccggaaaccc tgggccaggc atcgcgcctg tccggcatga
     2761 cgccggcggc ggtgtcgctg ttgttggtct atctgaaacg cgccggcaac gatgcccgaa
     2821 agacttcgca tgacgcttga acaagaactg gaagccggcc tggcgcaact gggcctggaa
     2881 ttgtccgccg agcaaatcga ccgcctgaac caatatctgg cgctgctcaa taaatggaac
     2941 aaaacctata acctcaccgc cgtgcgcgaa accgagcgca tggtggccta ccacgtgctg
     3001 gacagcctga gcgcgctgcc gcacatccag ggcgtgcgcg tgctggacgt cggctccggc
     3061 ggcggcatgc ccggcatgct gttcgccatc gcccgcccgg actggcagct gaccctgatc
     3121 gacgccaacc acaaaaaaac caccttcctg cgtcaggcgg cgattgagct ggggctgaac
     3181 aatgttgaag tgcattgcga acgcgtcgag gcgctggcgg cgccggcgtt tgacgtgatc
     3241 acctcgcgcg cctttgccga tctggccgag tttgtgcgcc tgacccgcca tgtgctggcc
     3301 gacggcggcg tgtgggcggc gctcaaaggc gtgtatcctt acgaggaaat cgcccagctg
     3361 cccgatgaca tccgcgtggt gtcggtgcag gcgctgcatg tgccggggct ggatgccgag
     3421 cggcatctgg tcaccctggc ccgcgtttaa tcaggaagga cgtttggcat ggccacggtt
     3481 attgcaatca ccaaccagaa aggcggcgtc ggcaagacca ccaccgtggt caatctggcc
     3541 gcgggttttg ccgccaaagg gctgcgcgtg ctgctggtcg atctcgaccc gcaggccaac
     3601 gccaccatgg gctgcggcat cgacaaggct aaattgccgg ccggcggtct gtatcgcctg
     3661 ctgctgggcg aagcgacgct ggaggcggtg cgctgccgct cggaaagtgg cggttttgac
     3721 gtgctgccgg ccaaccgcga gctgggcggc gccgaagtcg aactggtgtc ggccccgcgc
     3781 cgcgaacagc ggctgaaaga cgccctggcc gaagtgcgcg ccgactatga cttgattctg
     3841 ctggattgcc cgccggcgct gaatctgctg acgctcaacg gcctggccgc cgccagccgg
     3901 gtgattatcc cgatgcagtg cgagtattac gcgctggaag gcttgtccga cctggtcaac
     3961 accctgaaga aagcccgcgc ctcgctgaat ccgggcatcg agatgcttgg cttgctgcgc
     4021 accatgtacg acccgcgctt taccctgtcg cagcaggtca gcgcccaatt gcagcaatat
     4081 ttccctggca aactgttcga gacggcgatt ccgcgcaatg tgcggctggc ggaagcgccc
     4141 agccacggca tgccggcgct ggcgtatgat cgcgcctcgc gcggggccaa agcgtatctg
     4201 gcgctggtgg aagaaatcgc ccagcgcctg catctcaccc ttcccaagca ggtgggataa
     4261 atcatggcca aacccaaagg actgggccgc gggctggatg ccctgctcgc ggtcaatcat
     4321 agcgacgatc agctgcaaac cctgccgatt cacgccatcg agccgggcaa gtatcagccg
     4381 cgcacgcata tggacgccgc cgcgctggaa gcgctggccg agtcgatccg cgcccagggg
     4441 gtgattcagc cgctggtggt gcgcccggtg ggctacgagc gctacgagct gattgccggc
     4501 gaacggcgct ggcgcgccag ccagctggcc gggctggccg aagtgccggc cgtggtgcgc
     4561 accgtgccgg acgaagccgc gctggcgatg gcgctgatcg aaaatattca gcgcgaagac
     4621 ctgaatccgc tggaagaagc gctgggtttg aagcggctgg ccgacgagtt tggcatgacg
     4681 cacgaggcgg tggccgccgc ggtgggccgc tcgcgcagcg cggtgagcaa tctgttgcgc
     4741 ttgctgtcgc tggccgagcc ggtgcaggcc ttggtgaacg acggcaagct ggatatgggc
     4801 cacgcgcgcg ccttgctcac cctgccggtg ttgcagcaag tcagtctggc gcaggacatt
     4861 gcggaacgag gcttgtcggt gcgcgacgcc gagcagcgcg cccagcagct cttgcatccg
     4921 ccggcgccgc gcagtgaggc gcgggccgat ccggacgtgt tgcgcctggc ggaagaggtg
     4981 tcagaaaagg tgggcgccaa ggtcagcatc cggcctgggc gcaagggcag cggcaagctg
     5041 gtggtcgaat atgcgacttt agaccagctt gacaacttga tcgccgcgct gagcggccgt
     5101 cagtgaaatt gccaagcctt tcagatgttt aaaatcggcg cggtcttgta taagaccgcg
     5161 caattcattg acgtgacgga tgccctgtga gtataatctc tgagttcgtg cttgggctgc
     5221 ccaagcaact ggctgacgct gtaagcaaat tgttgcgctg gcaaattcgc ctgtcgctgc
     5281 cggtggtgat tgtgagcggg attctcggca tgccctcgtg gcacgcgccc gtgtctgccc
     5341 tccttggcgc gcttgtcgga attgtgccgg cgctggtgta tgtgcgcatt gcctaccgca
     5401 agccgcgggg tgcgcccgga aaactgctgt ccgcgcactt tgccgccgaa gccgccaaac
     5461 tggccgtgac cggcctgatg tttgccctgg tgctcgcgct ctataaggat gttgtgccat
     5521 tagcgctgtt ttcaagtttt tttgcaacgc tggtggccta ttggatcgcc cttctatcta
     5581 aataaatcga gaacgtgtta tggcaggctc cgctactgac tacatcaagc accacctgac
     5641 ccacgccaag gcgccgcatg acggcggttt ttggtcgctc aacgtcgaca ccttcagcgt
     5701 gtcgctgatt ctgggtttca tcttcctgtt tgtattcgcc cgcgtcgtgc gcaatttcag
     5761 cgtcgaaaat ccgggtcgtc tgcaatgtct ggtggaatcc atcgtcgaga tggtcggcaa
     5821 ccaagtgcgc gatattttcc acgccaagag cgatatggtg gccccgctgg ccctgaccgt
     5881 gttctgctgg gtgttcctga tgaacgccat ggacatgctg ccggttgacc tcctgcccta
     5941 cattgcccaa ctgatcggcg gcgccatggg tgctgatccg catcacgttt atctgcgtgt
     6001 ggtgccttcc gctgacgtca acgccacctt cgccatgtcg ctgtcggtga tgttcctgat
     6061 cattggcttc tcgatcaagg ccaagggttt tggcggctac atgcacgagt tgttttccgc
     6121 accgtttggc ccgaaagttg gcccgatcaa cttcatcttc cagctgatcg agctgatcgc
     6181 caagccgatt tcgctggccc tgcggttgtt cggcaatatg tatgccggtg aactgatttt
     6241 catcctgatt gctctgctgc cgtggtatgt gcagtggctg ctgggcggtc cgtgggccat
     6301 cttccacatc ctggtgatca cgctgcaagc gttcgtgttc atgatgttga ccatcgtgta
     6361 cctgagcctg gcggtggaag accactgatt taaaacgcga ttttcgcaat taacggtttc
     6421 tcaaccatcc ttctgtaaat ccttaggaga tataaatgga agcattcgtt tcgcagattc
     6481 agggcatgac cgcactggcc gccgccatca tgatcggctt gggcgccatc ggcaccgcac
     6541 tgggtttcgg cattctgggc ggcaagttcc tggaaagctc ggcccgccaa ccggaaatga
     6601 tcccggttct gcaaaccaag ctgttcatta tcgccggtct gcttgacgcc atctcgatga
     6661 tcggtgtggg tgtgggtctg ctgtacacct tcaacaaccc gttcctgacc gctggtgttg
     6721 ccgctctgaa ggctgctgct ggtcactaat ccgccgcaag tccggttgtt gtaaacactc
     6781 tgggggaaaa caagcgtgga tatcaacgca acactgctgg gacaggcgct cacattcgcc
     6841 gtcctggtgt ggttcaccat gaaatttgtt tggcctccgc tgaccaacat gatggacgag
     6901 cgcgccaagc gcattgctga tggcttggcc gctgcggaac gcggtaaaca ggatctggaa
     6961 gaagcatctc gtcgcgtcga agctgaaatt cgcgccgccc aacaaaaggc gaccgaaatg
     7021 ctcatcaacg ccgagaagcg cgccaaccag atcgtcgaag aagcgaaaca ggctgctcgg
     7081 accgaaggcg aacgcatcat tgctgacgcc aaggccgaaa tcgatcaaga agtgctgcgc
     7141 gccaaggaat ccctgcgcag ccaagtggct ggtcttgctg tcgccggcgc agagaaaatc
     7201 ctgcgcaagg aagtcgacgc agccaaacac gctgatctgc tagcctccat caaagcggag
     7261 ttgtaattaa ctcatggcag aaatcattac cgtagcgagg ccttacgccg aagcggtatt
     7321 ccggttggcc aaagacgcaa atcgactcgc cccttggggc gaggccctcg ccgtccttgc
     7381 tgccatgatg gagcactcgg aggtggtgac cctggtcacc aaccccaaga gcaccgctca
     7441 agaggtcgga aagctgatcg tcgatgtgct gggtgaccgc gccgacgttg aagtgaaaaa
     7501 cttcatcgac gcgctgcagg aaaaccaccg tctgacgttg ctaccggaaa ttgccagaca
     7561 gtttgaactc ctgaaatcgc gtgaagagag tgtggtggaa gccacagtcg aatccgcctt
     7621 tccgctcaac gaccaacagg cccaggagct tgccggcacg ctcaaagcga agttcggcaa
     7681 ggatgtccgc ctgtcaacga gcgtcgaccc cgacctgatc ggtggggtgc ggatcaaggt
     7741 gggcgacgat gtcattgacg cctcggtgcg cggcaaactg caagcaatgg cggtaagcct
     7801 caaaagttag gagagatcat gcagttaaac ccttctgaaa tcagcgagct gattcgcgcg
     7861 aagattcaga acttgccgtc gagcaccgaa gtgcgcacca cgggcaccgt ggtgtcggtg
     7921 actgacggca tcgtccgcgt ccacggcctc gccgatgtga tgcagggcga aatgctcgaa
     7981 ttcccgggca acaccttcgg tctcgccatg aacctcgagc gtgactcggt gggcgccgtg
     8041 gtgctgggcg aatacgagca catcagtgaa ggtgacgaag tcaagaccac cggccgcatt
     8101 ctggaagtgc cggtcggccg cgaactggtg ggccgcgtgg tgaacgcgct gggccaaccg
     8161 atcgacggca aaggtccgct gaacaccaag ctgtcctcgc cgattgaaaa aatcgcgccg
     8221 ggcgtgattg cccgtaaatc ggttgaccaa ccgatgcaaa ccggcctgaa ggccatcgat
     8281 tcgatggtgc cgattggccg tggtcagcgt gagctgatca ttggtgaccg tcaaaccggt
     8341 aaaaccgctg ttgccctgga tgccatcatc aaccagaagg gcactggcgt tgtgtgtatc
     8401 tatgtggcag tgggccaaaa agcctcctcc atcgccaacg tggtgcgcaa gctggaagaa
     8461 cacgatgccc tgggtcacac catcatcgtt gccgcttccg cttccgaagc cgccgcgctg
     8521 caatacatcg caccgtactc cggttgcacc atgggcgaat acttccgcga tcgcggcgaa
     8581 gacgccctga tcgtgtatga cgatctgtcc aagcaagccg tggcctaccg tcagatctcc
     8641 ctgctgctgc gccgtccgcc gggccgtgaa gcctacccgg gtgacgtgtt ctatctgcac
     8701 agccgcctgc tggagcgcgc tgctcgcgtg aacgccgatt acgttgaaaa gttcaccaac
     8761 ggtgaagtga aaggccagac cggttcgctg accgccctgc cgatcatcga aacccaagcg
     8821 ggtgacgtgt ccgccttcgt gccgaccaac gtgatctcga ttaccgacgg ccagatcttc
     8881 ctggaaagcg acctgttcaa ctcgggcatc cgtccggcta tcaacgccgg tatttcggtg
     8941 tcccgcgtgg gtggcgctgc acagaccaag gtcatcaaga agctgggcgg cggtatccgt
     9001 ctggccctgg ctcagtatcg cgaacttgcc tcgtttgccc agtttgcctc tgacctggac
     9061 gaagccaccc gcaagcagct gcaacacggt gaaatcgtga ccgagctgat gaagcaaaag
     9121 caattctcca ccctgaaggt gtcggagatg gcgctgacgc tgttcgcggt gaccaagggt
     9181 ttctacgaag gcgtgccggt caaaaaggcg ctggcgttcg aagcggcttt cctgtcgcac
     9241 atgaaggcca atcaagccgc tgtgctgtct gccattgaag cttccggtga attgtcgggc
     9301 gacaacgaga aaatccttgc caaggaactc gagtcgttca aagccggctt caacttcaac
     9361 tgatcctttc ggataacaga ctgaaaggaa cagggaatgg cagtcggcaa agagattcgc
     9421 accaagatca agagcgtgca gaacacgcaa aagatcaccc gcgccatgca gatggttgca
     9481 acgtcgaaaa tgcggaaaac gcaggatcgc atgcgcgcag ctcgtcctta cgccgaaaag
     9541 gtgcgccagg tcatggcgca ccttgcccag accaaccctg acgcgaacaa tccgctgctg
     9601 gccaagcgca gcagcgtcaa gcgcgccggt gtgatcctga tcacctcgga caagggtttg
     9661 gcgggcggcc tgaacgccaa cctgctgcgt cggttctgcc agagagtgga cgaactgatg
     9721 gggcagggcg tgcaggttga cgtatgtgcg ctgggccaaa aaggcctggc tgctgcgcag
     9781 cgcctgaagc tgaacgtcgt ggccagcgcc acccaactgg gtgacacccc gcaaatggat
     9841 aaattgatcg gcccgatttc ggtgatgatc aatgcctacg cggaaggcac catcgacgaa
     9901 ctgcacatcg tttacacccg cttcgtgaac accatgaagc aggagccgac gctggagcaa
     9961 ctgctgccgc tctcgaccaa acacatggct gttgagcatt cccacccgtg ggattacctg
    10021 ttcgagccgg acgtgaagtc ggtccttgac ttcctggtcc gccgctatct ggaatcggtg
    10081 atttaccagg gtgtggccga gaacatggcc tcggaacagg ccgcccggat ggtggccatg
    10141 aaggccgcca cggacaatgc agccaatctg atcgagagcc tcaagctcac ttacaacaag
    10201 gcccgtcagg cagcgattac cacggaactt tccgaaatcg tcgcgggcgc cgctgcggtg
    10261 taaggcgcgg gcaaccgata tattttttag gaaccgataa tgagccaagg caaaatcgtt
    10321 cagatcattg gcgcggttat tgacgtggag ttcccgcgcg atgccatgcc taaggtctat
    10381 gacgcattga aggtgctcga aacgggtctg acgctcgaag ttcagcaaca gctgggtgac
    10441 ggcgtggtcc gtgcgatcgc catgggtagc tccgacggtc tgcgtcgcgg cttggcagta
    10501 gccaacagcg gtgcgccggt gtctgtcccg gtgggtaagg ccactctggg ccgcatcatg
    10561 gacgtgctgg gtgacccgat tgacgaaaag ggcccgatcg gcgaagaaaa gcgcatgccg
    10621 atccaccgca aggccccggc attcgacgag ctgtccgccg ccaccgaact gttggaaacc
    10681 ggcatcaagg tgatcgacct gctgtgcccg ttcgccaagg gcggtaaagt gggtctgttc
    10741 ggcggcgccg gtgtgggcaa gactgtgaac atgatggagc tgatccgtaa catcgccatc
    10801 gaacacagcg gctactcggt gtttaccggc gtgggtgagc gtactcgtga gggtaacgac
    10861 ttctaccacg aaatgaacga ctccaacgtg ctggacaaag tgtcgctggt gtacggtcag
    10921 atgaacgaac cgccgggcaa ccgcctgcgc gtggcgctgt ctggcctgac catggccgag
    10981 aacttccgtg acgaaggtcg cgacgttctg ctgttcgtgg acaatattta ccgttacacc
    11041 ctggccggta ccgaagtgtc cgcgctgctg ggtcgtatgc cttctgctgt gggttaccaa
    11101 ccgaccctgg ccgaagaaat gggtaaattc caggagcgcg tggcttcgac caaggcgggt
    11161 tcgatcacct ccatccaggc cgtgtacgtg cctgccgatg acttgactga cccgtccccg
    11221 gccaccacct tcgcccactt ggacgccacc gtggtgctgt cgcgtgacat cgccgccctg
    11281 ggtatctacc cggccgtgga tccgctggac tccacctccc gtcagctcga tccgctggtg
    11341 gtgggtgaag aacactacaa cgtggcgcgc ggcgtgcaga tgactctgca aaagtacaag
    11401 gaactgcgtg acatcatcgc gattctgggt atggacgaac tgtctgaaga agacaaactg
    11461 accgtggccc gtgcgcgtaa gatccagcgt ttcctgtcgc agccgttctt cgtggcggaa
    11521 gtgttcaccg gctcgccggg caagtacgtg tcgctgaagg acaccattgc tggcttcaag
    11581 gcaattctca gtggcgagta cgatcacctg ccggaacaag cgttctacat ggtgggtggc
    11641 atcgacgaag tcatcgagaa ggccaaaacc gtctaatgga aggggcttgt tatggctagt
    11701 acgatgcatg ttgacgtggt cagcgccgaa gcgcagatct attccggcga agccgagttc
    11761 ctcgtggctc ccgctgaaat gggcgagatt ggcgtgtatc cccgtcacgt gccgctactg
    11821 acccgtatca agcccggctc gctgcgcatc cgcgtaccgg gccaggccaa agaagtgctg
    11881 gtggtggtgt cgggtggcat gatggaagtg caacccagcc tcatcaccgt gcttgccgat
    11941 acggccattc gcggcgagga gctcgacgag caacgcgcac aagcggccaa acgcgatgcc
    12001 gaggcggcgc tgtccaaggc caccgacgac caagagaccg cgcaagctcg cgcggcgctc
    12061 aaggcggcaa ttgccgagct gcacgcactc gagtacctgc gcaagcgggt gcactaaaac
    12121 actgttctgt gcgccatctg gcgggcagaa ccgggtttgc aggccaccgt cacagacggt
    12181 ggcctttttg tttgttatgc tatcggcgag aatgttgtga ctgaattcca gcatgcgtcg
    12241 ccgtcgtgcg gcgcgtttgt cgaggccgta tgaaccctgt ttctgttgtc attcttgccg
    12301 ccggtcgcgg caagcgcatg tattccgacc tgcccaaagt gctgcacccg attgccggca
    12361 agcccatgct ggcccgcgtc attgatctgg ccctgtcgct gttgcccagt cagtgcgtgg
    12421 tggtgattgg ccacggcgcc gaacaagtca gcgccgcttt tgcgggccat gccggcccgc
    12481 tggcgtttgc gctgcaaagc gagcaattgg gcaccggcca cgccgtcaag atggcgctgc
    12541 cgcacctgcc cagcgaaggg cgcaccctgg tgctgtacgg cgatgtgccg ctgaccgagg
    12601 ccgccaccct gcgccgcctg ctggacaccc cggccgatga cgtcgccctg ctcaccgaca
    12661 ccctggtcaa tccgaccggc tatggccgca tcgtgcgcga cgccgccggg cgcattcagt
    12721 gcatcgtcga agaaaaagac gccagcgccg agcaaaaagc cctgtgcgaa atcaacaccg
    12781 gcattgtcgc cctgcccaat gcccgtctgg ccggctggtt ggccgagctg aagaacggca
    12841 acgcccaagg cgaatattat ctgaccgacg tcatcggttt ggcggtgcgc gatggcgtca
    12901 ccgtgcatgg cgttggcgcg ccggcctcct ggcaggtggc cggggtcaac aacaaggcgc
    12961 agctggccga actggagcgg atttttcagg ccaaccaggc ccgcgtgttg ctggacgctg
    13021 gcgtgacgct ggccgacccg gcgcgcatcg acattcgggg cgagttgcgc tgcggtcggg
    13081 atgtcagcat cgacgtcaat tgcgtgtttg aaggcgacgt tactctgggc gatggcgtca
    13141 gcatcggccc caactgcgtg ctgaaaaacg tcagcctggc cgctggcgtg caggtggcgg
    13201 cgttttcgca tctggatggc gcgcaagttg gcgccgacgc ccgcatcggc ccgtttgccc
    13261 gcctgcgccc cggcgcgcgg ctggctgagg acgtgcacat cggcaatttt gtcgaaatca
    13321 agaacagcgc gctgtcggcc ggggccaagg tcaatcatct gagctatgtc ggcgacgccg
    13381 acgtgggcgc caaggtcaac gtcggcgccg gcacgattac ctgcaactac gacggcgtga
    13441 acaaattccg caccaccatc gaagccgggg cgtttattgg ctcgggcacc atgctggtgg
    13501 ccccggtcac cgtgggcgcg ggcgccacca ttggcgccgg ttcggtgatc agcaaggatg
    13561 ccccggccga gcagctgacc gtggcgcgcg ccaagcagct cagcctcagc ggctggcagc
    13621 gcccgctgaa aaaaacctga ttctggctgg cgtgcgccgc gcggctgccc acataggcgc
    13681 agccgcgccg gccgcgtaaa atgccggtat tcccaattat tttcccttgc aaaggttttc
    13741 ccatgtgtgg agtcgtcggc gccatcgcgc aacgtaatgt cgtgcccatc ctgctggaag
    13801 ggctgcggcg gctggaatat cgcggctatg actcggtggg cgtcgccgtg gtgggcgagc
    13861 agggcctggc gcgcgagcgc agcaccgaac gcgtggctga gctggccgaa caaacccgcc
    13921 gcgtgggcct gaaaggcttg accggcattg gccacacccg ctgggccacc catggcgcgc
    13981 cgtgcgagcg caacgcccac ccgcattttt ctcatggcct gctgtcgctg gtgcacaacg
    14041 gcatcatcga aaaccaccag ccgctgcgcg aacgcctggc ggcgcagggc tatgcgtttg
    14101 aatcagacac cgacaccgaa gtcatggtgc atctgatcca ctcttactac gtcacccatc
    14161 acgacctgct gcgcgccgtc cagcaaaccc tggccgagct gcacggcgct tacgccatcg
    14221 gcgtgattgc cgccgacaat ccggacgtga tggtctgcgc ccgccacggc agcccgctgc
    14281 tgctgggcct gggcattgaa gaaaacttct ttgcttccga cgtctccgcc ttgctgccgg
    14341 tcacgcaaaa agtgatttac ctggaagaag gcgaagtcgc ccggctgacg ctgaccgagg
    14401 tgcaggtgtt caatcaggct ggcgagcgtg tcgagccggt ggtgcatgtg tccgaacaat
    14461 cggccgatgt caccgagctg ggcgaatacc gccactacat gcaaaaagaa atccacgaac
    14521 agccgcgcgc gctggccgac accctggagc gggtgggcaa caccatcgag cccagcctgt
    14581 ttggcgccga agcgccggcc ttgttcgccg acatcaacgc cgtgaccatc atcgcctgcg
    14641 gcaccagcta tcacgccggc ctggtggcca aatactggct ggaaagcctg gccgggctgc
    14701 ccaccagcgt ggaagtggcc agcgaatacc gctaccgcga cagcgtggcc aacccctcgg
    14761 cgctggtggt caccatctcg caatccggcg aaaccgccga caccctggcc gcgctcaagc
    14821 acgccaaggc gctgggccac gacaagacgc tgagcatctg caatgtgccg gaaagctcgc
    14881 tgatccgcct gtcggcgctg cgttttctga cccacgccgg cccggaaatc ggcgtggcct
    14941 cgaccaaggc cttcaccacc cagctggtgg cgctgtacct gctgaccctg gcgctggcca
    15001 aggtgcgcgg ccgcctgagc gccgaacgcg aagccgacgc gctggccgac ctcaagcgcc
    15061 tgcccggcgc ggtgaacgcg ctgctggcgc tggaaagcgg gctggagcaa tgggccgaag
    15121 cgctggccac ccgccagcac gcgctgttcc tgggccgtca caccctgttc ccgattgccc
    15181 tggaaggcgc gctcaagctc aaggaaattt cttacatcca cgccgaagcc tacccggccg
    15241 gcgagctcaa acacggcccg ctggcgctgg tggaccgcga catgccgatt atcgtctgcg
    15301 cgcccaatga cgcgctgttc gacaagctgg cgtccaatat gcaagaggtg cgcgcacgca
    15361 acggcgaact gtttgtgctg tccgacgccg ccgttgaagc cggccccggc cagcatgtgc
    15421 tggccctgcc cgccggcatg cgcgacctga acccgctgct gtacaccatc ccgctgcaat
    15481 tgctggccta tcacaccgcc attcgcaagg ggac
//
LOCUS       DAOPYX010000029        15500 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_4093_length_15500_cov_7.964649, whole genome shotgun sequence.
ACCESSION   DAOPYX010000029 DAOPYX010000000
VERSION     DAOPYX010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 15500)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15500)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15500
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4093_length_15500_cov_7.964649"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(112..1011)
                     /locus_tag="PKN55_02750"
     CDS             complement(112..1011)
                     /locus_tag="PKN55_02750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018151246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNX70196.1"
                     /translation="MGQRMLASAEWLLLAVALVWGTSYGVAKLALEHYPVFGVLAVRF
                     ILAGLCLLPALRAIRWQAWRAGLPMGGILLAILLCETWALQYTSASNAAFLISLCLVL
                     TPLAEWLLYGQRPAARIWALVGLSWLGALLLTGDSAMHFSGGDALMLLAAVLRAFGVC
                     WLKQLADRQPATAQALTAMQCWTVGLGALALSASQGGLPPLPRDPGFWLAISYLALLC
                     TVCAFAIQNYALRRSDPSRVALLMGSEPVFGALFAWLWLGEAMGPLRWLGGGLMAVAA
                     VMATGGGSRVEKEVGEKKEIRFT"
     gene            1236..2168
                     /locus_tag="PKN55_02755"
     CDS             1236..2168
                     /locus_tag="PKN55_02755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018151248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HNX70197.1"
                     /translation="MDTNKLLGVLPDLLVLVKVVESGSFSAAGRALGCTPSAVSRQMA
                     RLEQALGSRLLERTTRSLRLTEAGQLAFQHSQAMLESAQAVLDSAGAHQAEPSGRVRL
                     AMPKAFGRRVIHPLMPAFLARYPKVDVQLLLDDHARDPISDEVDLVVLATDRPPPGLV
                     ARPLMPVAQWLCASPEYLARCGEPAQPQALQDHDCLFLGETPDDCVWEFARDGQRQRV
                     RVSGRYIVNHSDIRRDAVLRGLGIASLPQFVAADDVRAGRLRRVLADWECIPHAYWGH
                     AYLLYPAHRQLPRKVRVMIDYLLETVRDPAAGVA"
     gene            complement(2176..2706)
                     /locus_tag="PKN55_02760"
     CDS             complement(2176..2706)
                     /locus_tag="PKN55_02760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX70198.1"
                     /translation="MNRRLAYAALLGLGLLGWAAPAAARYQDPTLRFSLELPPGWTAR
                     ARPPGAPADNTVRLRPPRRTDRDRGQVRVDVWPGPVLKPAALARHLADIGSDDDAAVL
                     ARRPASARQPIHWTEYRIGMYGADGEWRIERRLEGVQAVPGQTWHIRCTAAESEFRHY
                     RYLLEGICLSFVPPKR"
     gene            2847..4367
                     /gene="gpmI"
                     /locus_tag="PKN55_02765"
     CDS             2847..4367
                     /gene="gpmI"
                     /locus_tag="PKN55_02765"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008954892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HNX70199.1"
                     /translation="MNAITPVLLLILDGFGHREEGEDNAILHANTPNWDALRARNPYS
                     VLDASEHAVGLPGGQFGNSEVGHLNIGAGRVVQQDISRIDTDIEQGSFANNPVIADAL
                     NRAKASRLHVFGLLSDGGVHSHENHIHALLRAAQAAGVGEVCLHAFLDGRDTPPRSAQ
                     TYLQRLDSVLAECPNARLVSVCGRFWAMDRDKRWERVQTAYRLVVDGEGEFHADTGLA
                     ALAAAYARDENDEFVKPTAIGAPCRIDDGDVVLFMNFRADRARELSSALTDPAFDGFA
                     ARQPKLALFASVTSYGEAYSHPVAYPPQTLKNGLGEYLAGLGLKQLRIAETEKYPHVT
                     YFFNGGEEQVYPGEDRILVQSPKVATYDLQPEMSAPEVADKIVQAIASGQYQAIICNF
                     ANGDMVGHTGNFPAAVKAIEALDACVGRCVEAMRAAGGEVLITADHGNAEQMFDHGHD
                     QPHTQHTLNLVPLLYVGRAAQLQSGGALRDIAPTLLAMMGLPQPAEMTGRSLIQFQ"
     gene            4378..5808
                     /locus_tag="PKN55_02770"
     CDS             4378..5808
                     /locus_tag="PKN55_02770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008954891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HNX70200.1"
                     /translation="MKPSTPLLLILLPALAMAAPPAGDATQAPKQDLKDVRQQINALE
                     KEVKAKEANRAEATDALRASEVAISEANRALASLNEQQSHSEQQLAKVRANIQQAQRD
                     VAVSRKRLGRILNAQYRHGQNDALTVLLNQQDPNQTQRDLAYYRKIAQAQQQVGQQLK
                     TQLAELERLSGQLETEKAKLDDIAASRVEHKQKLVKEKENRQQVVSQLSNQIQQQRQQ
                     LGKLQADEQRINALIDKINRELEAQRREAARKAEEARKLAQAKHEQAVREAKAKAERE
                     AKAQREAAEREAKARHARDLAAAQAAAKAGKPAPPPEPAPPPPPPPKAEPKAEPPAKA
                     VDDVADDSSSGRAFASLQGKLRLPVRGEITGRFGAARAEGMTWKGVFIRAGGGQPVRA
                     VADGRVVYADWLRGFGNMLIVDHGGGYMTVYGSGEALMKSTGDKVRAGDTVATTGASG
                     GGSETGLYFEIRHLGRPLNPLSWAQS"
     gene            5886..7361
                     /locus_tag="PKN55_02775"
     CDS             5886..7361
                     /locus_tag="PKN55_02775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012698208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HNX70201.1"
                     /translation="MHTLQKMALVSAGAVAGAILSLSINAMADKEPANATTLPLTELR
                     TFTEVFGRIKQDYVEPVEDKKLITEAINGMLSGLDPHSSYLDPEAFKELREGTQGEFG
                     GLGIEIGSEDGLVKVIAPIEDTPAFRAGIKSGDLIVKIDEVSVRGLSINEAVKRMRGK
                     PGTKVTLTLARKGESKPLVVPLTRAVIKTKSVKFKMLEADYGYIRITQFQEHTTENLA
                     QALEALYKENAKPLKGLVLDLRDDPGGLLNSAVGVSAAFLPKDALVVYTDGRAPDAKM
                     RLTATPANYMREQSKQDYFQRTPAEVKTLPLVVLVNSGTASASEIVAGALQDHKRAVV
                     VGVKTFGKGSVQSVLPLGNQGGIKLTTARYYTPNGRSIQATGITPDVVVEEATLSGGA
                     ATPSLRIREADLEHHLSNPNGDKESKPEAAPKADKAEKPAAKPRDDKSKGDSKDEPAN
                     PRDPNPKTDNQLNQALTVLKVQQILPGGASPVKDAKPAASK"
     gene            complement(8117..8782)
                     /locus_tag="PKN55_02780"
     CDS             complement(8117..8782)
                     /locus_tag="PKN55_02780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008953931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HNX70202.1"
                     /translation="MAGKAAKGQAFKDALRPLRRALPKAAPAHIAPPPPPAPEPEPVF
                     SELFADVQPLKTDNRYPHRLPAPPHWPRHSAAQAHANPRPNAHITDSMVGWFEPATID
                     PSFVRAGMQRHTLKKLRQGHWPVVAELDLHGLSRFDAQQHLAVLLHRARQHGQCCVRV
                     IHGKGLGSREGLPVLKQVIRTWLRHHPHVLAFCEADDAQGGAGALLVLLRRDSEGLGP
                     DGG"
     gene            complement(8806..9756)
                     /gene="trxB"
                     /locus_tag="PKN55_02785"
     CDS             complement(8806..9756)
                     /gene="trxB"
                     /locus_tag="PKN55_02785"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HNX70203.1"
                     /translation="MSTPRHSRLLILGSGPAGYTAAVYAARANLNPVIITGLAQGGQL
                     MTTTEVDNWPADAQGVMGPELMQRFQQHAERFGTEMIFDHIHTAALTEKPIRLVGDSG
                     EYTCDALIIATGASAQYLGLPSEEAFMGRGVSACATCDGFFYRNKDVAVIGGGNTAVE
                     EALYLANIARHVTVVHRRDKFRSEKILADKLMARVAEGKVTLAMNHTLDEVLGDASGV
                     TGMRIKSTDDGATRDIELAGVFVAIGHKPNTELFVGQLDMDNGYLVTRGGRDGNATAT
                     NLPGVFAAGDVQDHIYRQAVTSAASGCQAALDAERYLDQL"
     gene            complement(10084..13578)
                     /gene="smc"
                     /locus_tag="PKN55_02790"
     CDS             complement(10084..13578)
                     /gene="smc"
                     /locus_tag="PKN55_02790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012698321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HNX70204.1"
                     /translation="MRLTHLKLAGFKSFVDPTTIPVPGQLVAVIGPNGCGKSNVIDAV
                     RWVLGESSAKQLRGESMQDVIFNGSSTRKPVSRASVELVFDNSEGRASGPWGQFAEIS
                     IKRVLTRQGESSYWINQQQVRRRDIADLFLGTGVGAKGYAVIEQGMISRIIEARPEEL
                     RAYLEEAAGVSRYKERRRETESRLSDTRDNLARIEDIRQELERQTEKLLEQAEVAQQY
                     HGLRDALTHKQNLLAMARRRDAQQAEAKARAELARLEAELEGMSSQGTELDARLEAAR
                     EAHFAATDAVHQRQAEQAEASAQLARLEEQQRHRRDTASRLQRDLDGVRQELAGLSQQ
                     ERQLNDEIEHWEDQLEEARLAAEEAALAHDGEDDGVPAAEALFRQADEALNTLTRRQA
                     ELTRQRDLARQQAQHAETALRRQAERRERLHDEARRLSGEDSEALSLAREALAQAETE
                     AEQAREVTQQAEAALEQLTRQRQERQALHTRQRDALTRLHADLTALDKLLAQAARAGR
                     GEAWLAEHGLAGQAPLWRQLDIQPGWETAVEAALAPLLSARAVDDWPSDAPPSRLALL
                     ARHADEPAAEAEPLPLPTLASKLSLRAPELTGALADWLRGGYVVDSLDELYRWQARLG
                     PGQWLVCRDGHRAMGASVAYWAADADSGMIGRQAERERLAAEHEALAPEVTALGEACA
                     ALDAELHDQQQALRQLRQHAASAEQARAQAALHCARQEHTQAQHAARRAAIDEEQARL
                     DEEAEQEHASIEHANLLAEEAELQLDELSLALEQAQVRRLDAETGLTLARTRSRELER
                     HAQEARFALSTAQNRLNELEHRRDDLSERRDTLNERQENLVIEQDSLIQSDLDELLQS
                     ALIVRDECEEKLIAARDAANHATELLRQLTQARQQVDGALDPLRDAINDWRLKSQEAL
                     LTVERFDEELRNAEADETALLADLTDAVKASGLVGEIGKLTQAITGLGAVNLAALDEL
                     TAARERHDYLAEQAADLTAAMDTLLEAIAKMDGETRDMLQSTFDQVNASMRELFPLLF
                     GGGRAELTLTGEDLLDAGVQIIAQPPGKKNSTIHLLSGGEKALTAMSLVFSLFRLNPA
                     PFCLLDEVDAPLDDANTGRFCELVKRMADRTQFLYISHNRLTMEMAEQLIGVTMQEQG
                     VSRIVAVDIVEALKMREQ"
     gene            13680..14240
                     /locus_tag="PKN55_02795"
     CDS             13680..14240
                     /locus_tag="PKN55_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HNX70205.1"
                     /translation="MDEFSLTDHFLIAMPNMTDPNFAGTVVYLCEHNEHGAMGVVVNR
                     PAEVSLGELFDQIDLSLPPGELPGQPVYLGGPVQTDRGFVLHQPLDNWNSTLAVSDDT
                     GLTTSRDILVAVSQGEGPYRMLVTLGYAGWGAGQLEQELAQNAWLTVPANLDCVFDLP
                     AQDRFRAAMQLLGFDPLALSGEAGHA"
     gene            14233..14688
                     /gene="ruvX"
                     /locus_tag="PKN55_02800"
     CDS             14233..14688
                     /gene="ruvX"
                     /locus_tag="PKN55_02800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003795480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HNX70206.1"
                     /translation="MPDVALPQGALLGFDFGQARIGVALGNTEVAIAHPLETITGDSN
                     DQRFARIGVLIQEWSPAALVVGLPTHADGTPHEMTRLARKFAQRLHGRFGLPVWLVDE
                     RHSSQVAEVQLNDAGLRGRRQKPALDQVAAQVILQSFLDMPQAAGIEPL"
     gene            14888..15499
                     /locus_tag="PKN55_02805"
     CDS             14888..15499
                     /locus_tag="PKN55_02805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX70207.1"
                     /translation="MLSEPHSFLAQAFAGPRSYLPMASHAYALSGNPLTMDDFHRRYL
                     LLNQYLADPHPLTQLKPEAIAPGLATLTCQQVIELLLHWPGAPQRNAILDAVCVEFGL
                     CPVDNSPFRIWGYYAPGQGALPLHVYIGCDGWLYDTLPGLPAHRTRDSHGRCPPSWLL
                     RHGMQPLDEAHVCSTAVHALAAGTLASIHAPEQAWRTVTEASG"
BASE COUNT         2630 a         5039 c         5176 g         2655 t
ORIGIN      
        1 cattgcttgt gtcgtgtact ttctcctgcc cgagcaccaa tccagcctga gtggcaaagg
       61 cactcaccat tgccagcgcc gcgagtcgtg cgctatatgt acgcaacggt tttacgtgaa
      121 gcgaatctct ttcttctcac ccacctcctt ctccacccta ctccctcccc ccgtcgccat
      181 caccgccgcg accgccatca gcccgccgcc cagccagcgc agcgggccca tcgcctcacc
      241 cagccacagc caggcgaata gcgcgccgaa caccggttcg ctgcccatca gcagggcgac
      301 gcggctgggg tcgctgcggc gcagggcgta gttctggatg gcaaaggcgc agacggtgca
      361 cagcagggcc aggtagctga tggccagcca gaagccgggg tcgcgcggca ggggcggcag
      421 gccgccttgg ctggcgctca gggcgagggc gcccaggccg acggtccagc attgcatggc
      481 ggtcagcgcc tgggcggtgg cgggttggcg gtcggccagc tgtttcagcc agcacacgcc
      541 aaaggcgcgc agcacggcgg ccagcagcat cagcgcgtcg ccgccggaaa agtgcatggc
      601 gctgtcgccg gtgagcagca gcgcgcccag ccaggacagg cccaccagcg cccagatgcg
      661 cgcggccggg cgctggccgt acagcagcca ttcggccagc ggggtgagca ccaggcacag
      721 gctgatcaaa aacgccgcgt tgctggcgct ggtgtattgc agcgcccagg tttcgcacag
      781 caggatggcc agcagaatgc cgcccatcgg caggccggcg cgccaggctt gccagcggat
      841 ggcgcgcagc gccggcagca ggcacagccc ggccaggata aagcgcacgg ccagcacgcc
      901 gaacaccggg tagtgctcca gcgccagttt ggccacgcca tagctggtgc cccacaccag
      961 cgccaccgcc aacagcagcc attcggcgct ggctaacatt cgttgtccca tcgtgttctc
     1021 cttgctgcgg cttacttcac ccggttggct tggccacccg actgtccgtc tggctggcca
     1081 tgcggggcgg tgttcgccgt gctgataaaa aagtgaaccc actcacccgc ctgagcaggg
     1141 gcgcggcgcg agtggcgaaa aattctcgat gggcagacga tacattgatg caaaaacggc
     1201 gataatcccg ttttttagaa cagtagctgt gcgccatgga cacaaataaa ctgctggggg
     1261 tgttgcccga tttgctggtg ttggtgaagg tggtggaaag cggcagtttt tcggcggcgg
     1321 ggcgggcgtt gggttgcacg ccatcggcgg tcagccggca gatggcgcgg ctggagcagg
     1381 cgctgggcag ccggctgctg gagcgcacca cgcgcagcct gcggctgacc gaggccggcc
     1441 agctggcgtt tcagcacagc caggccatgc tggaatcggc ccaggcggtg ctggatagcg
     1501 ctggcgcgca tcaggccgag cccagcggcc gggtgcggct ggccatgccc aaggcgtttg
     1561 gccggcgggt gattcacccg ctgatgccgg cgtttttggc gcgctacccc aaggtggatg
     1621 tgcaactgct gctggacgac cacgcccgcg acccgatcag tgatgaggtg gatttggtgg
     1681 tgctggccac cgaccgaccg ccgccggggc tggtggcgcg gcccttaatg ccggtggcgc
     1741 agtggctgtg cgcctcgccc gagtatctgg cccgatgcgg cgagccggcc cagccgcagg
     1801 cgctgcaaga ccatgattgc ctgtttctgg gagaaacacc ggacgattgc gtgtgggaat
     1861 tcgcccgtga cggccagcgc cagcgggtgc gggtgtccgg gcgctatatc gtcaaccaca
     1921 gcgacatccg ccgcgacgcg gtgctgcgcg gcctgggcat tgccagcctg ccgcagtttg
     1981 tcgccgccga tgacgtgcgc gccggccggc tgcgccgggt gctggccgat tgggagtgca
     2041 tcccccatgc ctattggggc catgcgtatc tgctgtaccc ggcgcaccgg caattgccgc
     2101 gcaaggtgcg ggtgatgatt gattacctgc tggagacagt gcgcgatccg gcagcggggg
     2161 tggcgtgatg aaagcctagc gttttggcgg tacaaagctc agacaaatac cttccagcag
     2221 atagcggtaa tggcgaaact cgctttcggc ggcggtgcag cggatgtgcc aggtttgccc
     2281 cggcacggct tgcacgccct ccagccgacg ttcgatgcgc cattcgccgt cggcgccata
     2341 catgccgatg cggtattcgg tccagtggat gggctggcgg gcgctggccg ggcggcgggc
     2401 cagcacggcg gcatcgtcgt cgctgccgat gtcggccaga tggcgggcca gggcggcggg
     2461 tttgagcacc gggccgggcc acacgtccac ccgcacctgg ccacggtcgc ggtcggtgcg
     2521 tcggggcggg cgcaggcgca cggtgttgtc ggccggcgcg ccgggcgggc gggcgcgcgc
     2581 ggtccagccc ggcggcagct ccaggctgaa gcgcagggtg gggtcctggt agcgcgccgc
     2641 ggcaggcgcg gcccacccca gcaggccaag ccccagcagc gcggcatacg ccaggcgccg
     2701 attcatatgg cagcagaaaa atcattcatg atgcagtgca ttatacgaag ttcgcgccgc
     2761 aggcatagca cgccgcgcgc gccgcgctgg aaaaacggta caatgccgcc atcttgtgtt
     2821 gacccgactg aacctgtccg atgcccatga atgcgattac cccggtgctg ttgttgattc
     2881 ttgacggttt tggccaccgc gaggaaggcg aagacaacgc catcctgcac gccaacaccc
     2941 ccaactggga cgcgctgcgc gcccgcaacc cctacagcgt gctggacgcc tccgagcacg
     3001 ccgtgggcct gcccggcggg cagtttggca attcggaagt cggccacctg aacattggcg
     3061 ccggccgcgt ggtgcagcag gacatcagcc gcatcgacac cgacatcgaa caaggcagct
     3121 ttgccaataa tccggtgatt gccgacgcgc tgaaccgcgc caaggccagc cgcctgcatg
     3181 tgtttggcct gctgtccgac ggcggcgtgc acagccacga aaaccatatt cacgccctgc
     3241 tgcgcgccgc ccaggcggcg ggcgtgggcg aggtgtgcct gcacgccttc ctggatggcc
     3301 gcgacacccc gccgcgcagc gcgcaaacct atttgcagcg gctggacagc gtgctggccg
     3361 agtgcccgaa tgcccggctg gtcagcgtgt gcggccgctt ctgggcgatg gaccgcgaca
     3421 agcgctggga gcgcgtgcaa accgcctacc ggctggtggt ggacggcgaa ggggaattcc
     3481 acgccgacac cggcctggcc gcgctggcgg cggcttacgc ccgcgacgaa aacgacgaat
     3541 tcgtcaaacc caccgccatt ggcgcgccgt gccgcatcga cgatggcgat gtggtgttgt
     3601 tcatgaactt ccgcgccgac cgcgcccgcg agctgagcag cgcgctgacc gacccggcgt
     3661 ttgacggctt tgccgcccgc cagcccaagc tggcgctgtt tgcctcggtg accagctatg
     3721 gcgaggcgta ttcccacccg gtggcctacc cgccgcaaac cctgaaaaac ggcctgggcg
     3781 aatacctggc cgggctgggg ctcaagcagc tgcgcattgc cgaaaccgag aaatacccgc
     3841 acgtcactta tttcttcaac ggcggcgaag aacaggtgta tccgggcgaa gaccgcattc
     3901 tggtgcagtc gcccaaggtg gccacctatg atttgcagcc ggaaatgagc gcgccggaag
     3961 tggccgacaa gattgttcag gccatcgcca gcggccagta tcaggcgatt atctgcaact
     4021 tcgccaatgg cgacatggtg ggccacaccg gcaacttccc ggcggcagtc aaggccatcg
     4081 aggcgctgga cgcctgcgtc ggccgctgcg tcgaggccat gcgcgccgca ggcggcgagg
     4141 tgttgatcac cgccgaccac ggcaacgccg agcagatgtt tgaccacggc cacgaccagc
     4201 cgcacaccca gcacacgctg aacctggtgc cgctgctgta tgtgggccgc gccgcccagc
     4261 tgcaatccgg cggcgcgctg cgcgacatcg cccccaccct gctggcgatg atgggcctgc
     4321 cgcagccggc cgagatgacc ggccgctcgc tgattcaatt ccagtgaccc tgcgcccgtg
     4381 aagccgtcta ctcccctgct gttgatcctg ctgccggcgc tggccatggc cgcgccgccg
     4441 gctggcgacg ccacccaggc gccaaaacag gatctgaaag acgttcgcca gcagatcaac
     4501 gcgctggaaa aagaagtcaa ggccaaagag gccaaccgcg ccgaagccac cgacgccctg
     4561 cgcgcttcgg aggtggccat cagcgaggcc aaccgcgcgc tggcttcgct gaacgagcaa
     4621 caaagccatt ccgaacagca gttggccaag gtgcgcgcca atatccagca ggcccagcgt
     4681 gatgtggccg tgtcgcgcaa gcggctgggg cgcattctga acgcccagta ccgtcatggc
     4741 cagaacgacg ccttgaccgt gctgctgaac cagcaagacc ccaatcaaac ccagcgcgat
     4801 ctggcgtact atcgcaagat tgcccaggcg cagcagcaag tcggccagca gctgaaaacc
     4861 cagctggccg agctggagag attgtccggc cagttggaaa ccgaaaaagc caagctggat
     4921 gacatcgccg cttcgcgtgt cgagcacaag caaaagctag tcaaggaaaa agaaaaccgc
     4981 cagcaggtgg ttagccagct gtccaatcag attcagcagc agcgccagca gttgggcaag
     5041 ctgcaagctg acgaacaacg catcaatgcc ctgatcgaca agatcaaccg cgaactggaa
     5101 gcccaacgcc gcgaagccgc gcgcaaggcc gaagaggcgc gcaagctggc gcaggccaag
     5161 catgaacagg ccgtgcgcga agccaaggcc aaggcggaac gtgaagccaa ggcgcagcgc
     5221 gaagccgccg agcgcgaggc caaggcccgc cacgcccgcg atctggccgc cgcgcaagct
     5281 gccgccaagg ccggcaagcc ggcgccgccg cccgaacccg cgccgccgcc tccgccaccg
     5341 cccaaggcgg aacctaaagc cgaaccgcca gccaaagcgg tggatgacgt tgccgatgat
     5401 tccagctctg gccgcgcttt tgccagcctg caaggcaagc tcaggctgcc ggtgcgcggc
     5461 gaaatcaccg gccgctttgg cgctgcgcgc gccgagggca tgacctggaa aggcgtgttt
     5521 atccgcgcag gtggcggtca gccggtgcgc gccgtggccg atggccgcgt ggtgtacgcc
     5581 gactggctgc gcggttttgg caatatgctg attgtggatc acggcggcgg ctatatgacc
     5641 gtatacggca gtggcgaggc gctgatgaaa tccaccggcg ataaagtacg cgccggcgac
     5701 accgtggcca ccaccggcgc ttccggcggc ggcagcgaaa ccgggctata tttcgaaatc
     5761 cgccacctgg gccgtccgct caaccccttg agctgggcac aatcgtaatc catcggcgca
     5821 gcccgccccc atgctgaatc gggctgcgca gtttgaagaa cacaccggca gcgccgggga
     5881 gaactatgca cactctgcaa aaaatggcct tggtcagcgc tggcgccgtg gctggcgcca
     5941 ttctcagcct gagcatcaat gccatggccg acaaagaacc ggccaacgcc accaccctgc
     6001 cgctgaccga gctgcgcacc ttcaccgagg tgtttggccg catcaagcag gattatgtcg
     6061 agccggtgga agacaaaaaa ctgatcaccg aggcgatcaa cggcatgctg tccgggctgg
     6121 atccgcactc gtcctacctc gacccggaag ccttcaaaga actgcgcgaa ggcacccagg
     6181 gcgagtttgg cgggctgggc attgaaatcg gctcggaaga cggcctggtg aaagtcatcg
     6241 cccccatcga agacaccccg gcgtttcgcg ccggcatcaa gtcgggtgac ttgatcgtca
     6301 agatcgacga ggtatcggtg cgcggcctga gcatcaacga agcggtcaag cgcatgcgcg
     6361 gcaagccggg caccaaggtc acgctgaccc tggcgcgcaa gggcgaaagc aagcccttgg
     6421 tggtgccgct gacccgcgcg gtgatcaaga ccaagagcgt gaagttcaag atgctggaag
     6481 ccgactacgg ctatatccgc atcacccagt tccaggaaca caccacagag aatctggcgc
     6541 aggcgctgga agcgctgtac aaagaaaacg ccaagccgct caagggcctg gtgctggacc
     6601 tgcgcgatga tccgggtggc ctgctcaata gcgccgtggg cgtgtccgcc gccttcctgc
     6661 ccaaggatgc gctggtggtg tacaccgacg ggcgcgcgcc ggacgccaag atgcgcttga
     6721 ccgccacccc ggccaactat atgcgcgagc aaagcaagca ggattacttc cagcgcaccc
     6781 cggctgaagt caaaaccctg ccgctggtgg tgctggtcaa cagcggcacc gcttcggcct
     6841 cggaaatcgt cgccggcgcg ctgcaagacc acaagcgcgc ggtggtggtg ggggtgaaaa
     6901 cctttggcaa gggctcggtg cagtcggtgt tgccgctggg caaccagggt ggcatcaagc
     6961 tgaccacggc ccgctattac accccgaatg gccgctcgat ccaggccacc ggcatcaccc
     7021 cggatgtggt ggtggaagaa gccacgctga gcggcggtgc ggcaacgccg tcgctgcgca
     7081 tccgcgaagc cgaccttgaa caccatctga gcaacccgaa tggcgacaag gaaagcaagc
     7141 cggaagccgc acccaaggca gacaaagcgg aaaaacccgc cgccaagccg cgtgacgaca
     7201 aatccaaggg agacagcaag gacgagccgg ccaatccgcg cgaccccaac ccgaaaaccg
     7261 acaaccagct gaatcaagcg ctgaccgtgc tgaaagtgca gcaaatcctg ccgggcggcg
     7321 ccagcccggt gaaggatgcc aagccggcgg ccagcaagta agccaccccc tggcggtttg
     7381 tgccagcaca cgcgccagca atcagccgcc gttcgacatg gccacatcac cacgcggatg
     7441 tggccatgtc atacacgccg ccccggagcc agcctcccgg gcggcgtgtt gtctgtccgg
     7501 taggcagtgc gcttcagcgc acaaagacat ggtaattttc cggaaatgct ctacactgca
     7561 aatcactctg atttcatcag gaacaggaat ggctgatagc caacctgcct ggccatatcc
     7621 atcagggatt caagtggcgc gggccgtgtg acccggattc gcccgccgcg ccgtatcatc
     7681 acttgcttga ggctgataac acgtatgacc gcccccgact ctgctttctg tgatcaagga
     7741 caacgcgccc ccggcgaaca agcctgccag gattgcggca ttattgatgc cctcaccggc
     7801 caaccggcgc gttgcagcaa ccccgtatac gaaacagcgc gtgaagcagg tgaacgcggc
     7861 aaggcagccc agattgccca actactggca gagggcggcc ctccttccct gtaagaacac
     7921 ccaaaagcca tgcgcccgct cccgtttatc cggggcgggc gcatggcttt tggcacggac
     7981 gatttgccca ggcgggcgac agccccagac cttgggcgcc actcaaaaaa tctgcctgtg
     8041 cacacggtct gtaacaatgt agtctgtgtg ttcagtaacc ttctgttctg gcggtttttg
     8101 tccgtcgtgc tcaggcttac ccgccgtctg gccccagccc ctcactgtcc cgccgcaaca
     8161 acaccagcaa ggctcccgcg ccgccctggg cgtcgtcggc ctcacaaaac gccaagacat
     8221 gcggatggtg acgcagccag gtgcggatca cctgcttgag caccggcagc ccctcgcgcg
     8281 agcccagccc cttgccgtga atcacccgca cacaacactg cccatgctgg cgggcgcggt
     8341 gcaagagcac ggccagatgc tgctgcgcgt caaagcggga caaaccatgc aaatccagct
     8401 cggccaccac cggccaatgg ccttgccgca gttttttcag ggtgtggcgc tgcatgccgg
     8461 cgcgcacaaa gctggggtcg atggtggctg gctcaaacca gccaaccatg ctgtcggtga
     8521 tatgcgcatt ggggcgtggg ttggcgtgcg cctgggcggc gctgtggcgc ggccagtgcg
     8581 gcggggccgg caaacggtgt ggatagcggt tgtcggtttt cagcggctgc acatcggcaa
     8641 acagctcgct gaacaccggc tccggctcag gggcgggcgg cggcggtggg gcgatatgcg
     8701 ctggcgccgc cttgggcagg gcgcggcgca gcgggcgcaa ggcgtccttg aacgcctgcc
     8761 ccttggctgc tttgcccgcc atcgccgccg ccccgcgcgc cgtgcttaca gctggtcgag
     8821 gtagcgttcg gcgtccagcg cagcctggca gccggacgcg gcgctggtga ccgcctgacg
     8881 atagatatga tcctgcacat cgccggcggc gaacacgccc ggcagattgg tggcggtggc
     8941 gttgccgtca cggccgccac gggtcaccag atagccattg tccatgtcca gctggccgac
     9001 aaacagctcg gtgttcggct tgtggccgat ggcgacaaac acgccggcca gctcgatgtc
     9061 gcgggtggcg ccgtcgtcgg tggacttgat gcgcatgccg gtcacgccgc tggcgtcgcc
     9121 cagcacttcg tccagggtgt ggttcatcgc cagggtgacc ttgccttcgg ccacgcgcgc
     9181 catcagcttg tcggccagga ttttttcgct gcggaattta tcgcggcgat gcaccacggt
     9241 gacgtggcgg gcgatattgg ccagatacag cgcttcttcc acggcggtgt tgccgccgcc
     9301 aatcacggcg acgtctttat tgcggtagaa aaacccatcg caggtggcgc aggcggacac
     9361 gccacggccc ataaaggcct cttccgacgg caggcccaga tactgggcag aggcgcccgt
     9421 ggcgataatc agcgcgtcgc aggtgtattc gcccgaatcg ccgaccagac ggatgggttt
     9481 ttcggtcagc gcggcagtgt ggatatggtc aaagatcatt tccgtgccaa agcgctcggc
     9541 gtgctgctga aaacgctgca tcagctccgg gcccatcaca ccttgggcgt cagccggcca
     9601 gttgtccact tcggtggtgg tcatcagttg gccgccttgc gccaggccgg tgatgatcac
     9661 cggattgagg ttggcgcgcg cggcgtagac ggcggcagtg taaccagcgg ggccggagcc
     9721 caggatcaag aggcggctgt ggcgtggggt gctcatgtgt gcggtcctgt tgggtgcaga
     9781 gtggcaagag atgcgccgcg ccgggcggat ggctgggcgc aaggcgggaa gccgcgcggc
     9841 gggcggggcg ttccgggaca atagtctagt attttattca agcaatggat gggtttcaat
     9901 aaatagtatt tttctatcga acgatagttt gaattgatgg cctgccagct tgctttgccc
     9961 cgccttgggc aggggagcaa aggactgcgc aggagccgcg cactcaccaa acccagtccg
    10021 caaacaacac aagacatacc gccacgccaa gggctacaac accaggtgat ttgtgcgcgg
    10081 cgcttactgt tcgcgcattt tcagcgcttc gacaatatcc accgccacaa tgcggctgac
    10141 gccctgttcc tgcatggtca cgccgatcag ctgttcggcc atttccatgg tcagccggtt
    10201 gtggctgatg tacaaaaact gggtgcggtc ggccatgcgt ttgaccagct cgcaaaagcg
    10261 cccggtgttg gcgtcgtcca gcggggcgtc cacttcgtcc agcaagcaga acggcgccgg
    10321 gttgaggcgg aacagcgaaa acaccaggct catcgcggtc agggcttttt cgccgccaga
    10381 cagcaggtga atggtggagt tctttttgcc gggcggctgg gcgatgatct gcacgccagc
    10441 gtccagcagg tcttcgccgg tcagcgtcag ctcggcgcgg ccgccgccaa acagcagcgg
    10501 gaacagctcg cgcatcgagg cgttgacctg gtcaaacgtg ctttgcagca tgtcgcgggt
    10561 ttcgccgtcc atcttggcga tggcttccag cagggtgtcc atggcggcgg tcaggtcggc
    10621 ggcttgctcg gccaggtagt cgtggcgttc gcgcgcggcg gtcagttcgt ccagcgccgc
    10681 caggttgacc gcgcccaggc cggtgatggc ctgggtgagc ttgccgattt cgcccaccag
    10741 accggaggct ttcaccgcgt cggtgaggtc ggccaagagc gcggtttcgt cggcctcggc
    10801 gttgcgcagt tcttcgtcaa agcgctccac ggtcagcagc gcttcctggc ttttcagccg
    10861 ccagtcgttg atggcgtcgc gcagcgggtc cagcgcgccg tccacctgct ggcgggcctg
    10921 ggtgagctgg cgcaagagtt cggtggcgtg gttggcggcg tcgcgggcgg cgatgagttt
    10981 ttcttcgcat tcatcgcgca caatcagcgc gctttgcaat agctcgtcca gatcgctctg
    11041 gatcaggctg tcttgctcga tcaccaggtt ttcctggcgc tcgttcagcg tgtcgcggcg
    11101 ctcgctcagg tcgtcgcggc ggtgttccag ctcgttcagg cggttttgcg cggtggacag
    11161 ggcaaagcgc gcctcctggg cgtggcgctc cagctcgcgg ctgcgggtgc gcgccagggt
    11221 gaggccggtt tctgcgtcca gccggcgcac ctgggcttgc tccagcgcca ggctgagttc
    11281 gtccagttgc agttcggctt cttcggccaa cagattggcg tgctcgatgc tggcgtgttc
    11341 ttgttcggct tcttcgtcca gccgggcttg ttcctcgtcg atggcggcgc ggcgtgcggc
    11401 gtgctgggcc tgggtgtgtt cctggcgggc gcagtgcagc gcggcctggg cgcgcgcctg
    11461 ttcggcgctg gcggcgtgct ggcgcagttg ccgcagcgcc tgttgctggt cgtgcaattc
    11521 ggcgtccagc gcggcgcagg cctcgcccag ggcggtgacc tccggcgcca gcgcctcgtg
    11581 ttcggcggcc agccgttcgc gctcggcctg gcggccgatc atgccgctgt cggcgtccgc
    11641 cgcccaatag gccacgctgg cgcccatggc gcggtggccg tcgcggcaca ccagccactg
    11701 gcccggcccc agccgcgcct gccagcggta tagctcgtcc aggctgtcca ccacatagcc
    11761 gccgcgcagc cagtcggcca gcgcgccagt cagctcgggc gcgcgcaggc tgagtttgct
    11821 ggccagggtg ggcaagggca gcggttcggc ttcggcggcc ggttcgtcgg cgtggcgggc
    11881 cagcagcgcc agccggctgg gcggggcgtc gctgggccag tcgtccactg cgcgcgccga
    11941 caacagcggc gccagggcgg cttctacggc ggtttcccag ccgggctgga tgtccagttg
    12001 ccgccacagc ggcgcctgcc cggccaggcc atgttcggcc agccaggctt cgccgcgccc
    12061 ggcgcgcgcc gcttgcgcca gcagtttgtc cagcgcggtc aggtcggcgt gcagccgggt
    12121 cagcgcgtcg cgctggcggg tgtgcagggc ttggcgctcc tggcgctggc gggtcagttg
    12181 ctccagcgcg gcttcggcct gctgggtgac ttcgcgcgct tgctcggcct cggtttcggc
    12241 ttgcgccagg gcttcgcggg ccaggctcag cgcctcgctg tcttcgccgc tcaggcggcg
    12301 cgcctcgtcg tgcaggcgct cgcggcgctc ggcctggcgg cgcagtgcgg tttcggcgtg
    12361 ttgcgcctgc tggcgggcca gatcgcgctg acgggtcagc tcggcctggc ggcgggtcag
    12421 ggtgttcagc gcttcgtccg cctggcggaa cagcgcctcg gcggccggca cgccgtcgtc
    12481 ttcgccatcg tgggccaggg cggcttcttc ggcggccagc cgggcttctt cgagttggtc
    12541 ttcccagtgt tcgatttcgt cgttgagctg gcgctcttgc tggctgagcc cggccagctc
    12601 ctggcgcacg ccgtccaggt cgcgttgcag gcggctggcg gtgtcgcggc ggtggcgttg
    12661 ttgttcttcc agccgcgcca gctgcgcgct ggcttcggct tgttcggcct ggcgctggtg
    12721 cacggcgtcg gtggcggcaa aatgcgcctc gcgggcggct tctagccggg cgtccagttc
    12781 agtgccctgg ctgctcatgc cctccagctc ggcttccagc cgcgccagct cggcgcgcgc
    12841 cttggcctcg gcctgttgcg cgtcgcggcg gcgggccatg gccagcaggt tttgcttgtg
    12901 ggtcagcgcg tcgcgcaggc cgtggtattg ctgggcgact tcggcctgct ccagcagttt
    12961 ttcggtttgc cgctccagct cctggcgaat gtcttcgatg cgggcgaggt tgtcgcgggt
    13021 gtcggagagg cgcgattcgg tttcccggcg gcgctctttg tagcgcgaca cgccggcggc
    13081 ttcttccagg taggcgcgca gttcttccgg gcgcgcctcg atgatgcgcg agatcatgcc
    13141 ctgctcgatc accgcatagc ccttggcgcc gacgccagtg cccagaaaca aatcggcgat
    13201 gtcacgccgg cgcacttgtt gctggttgat ccagtagctg gattcaccct ggcgagtcag
    13261 cacgcgcttg atggagattt cggcaaattg cccccacggc ccgctggcgc ggccttcgct
    13321 attatcgaac accagttcga ccgaggcgcg gctgaccggc ttgcgggtgg acgagccgtt
    13381 aaaaatcacg tcctgcatgg attcaccacg cagttgcttg gccgaggatt cgcccagcac
    13441 ccagcgcacg gcgtcgatca cattggattt gccgcagcca ttggggccga tgacggccac
    13501 cagttggccg ggcacgggga tggtggtcgg gtccacaaag gatttgaacc cggccagttt
    13561 cagatgagtc aggcgcacga aagcttcaca gtgccacagg aaattcagga atgcgtgatt
    13621 gtaacgaaaa ccaggtcagc gcgcgccgca agcgcagcag cctatgccat aatgctgaca
    13681 tggatgaatt ttctttgacc gatcacttcc tgatcgccat gcccaatatg accgacccga
    13741 actttgccgg tacggtggtt tacctgtgcg agcacaacga gcacggggcg atgggtgtgg
    13801 tggtcaaccg ccctgccgaa gtctcgctgg gcgaactatt cgaccagatc gacctctcct
    13861 tgccgccagg tgagctgccc ggacagccgg tgtacctggg cgggccggtg caaaccgacc
    13921 gtggctttgt gctgcatcag ccgctggaca actggaactc cacgctggcc gtctcggacg
    13981 acaccggctt gaccacctcg cgcgatattc tggtggcggt cagccagggc gaggggcctt
    14041 accgcatgct ggtgacgctg ggttacgccg gctggggcgc cggtcagctg gaacaagagc
    14101 tggcacagaa cgcctggctg acggtgccgg ccaatctgga ttgcgtgttt gatctgccgg
    14161 cgcaggatcg cttccgcgct gccatgcaat tgctgggctt tgacccgctg gcactgtcgg
    14221 gcgaggccgg ccatgcctga tgttgcgctg ccgcaaggcg ctttgctggg ctttgacttt
    14281 ggccaggcgc gtattggcgt ggccttgggt aataccgaag tggcgattgc ccatccactg
    14341 gaaaccatca ctggtgacag caacgaccag cgctttgccc gcatcggcgt cttgattcag
    14401 gaatggtcgc ccgccgcgct ggtggtgggc ctgcccaccc acgccgacgg caccccgcac
    14461 gagatgaccc ggctggcgcg caaatttgcc cagcgcctgc atgggcgctt tggcttgccg
    14521 gtgtggctgg tggacgagcg ccacagctcg caggtggccg aagtgcagct gaacgacgcc
    14581 ggcctgcgcg gccgccggca aaagccggcg ctggatcagg tggcggcgca ggtgattctg
    14641 caaagttttc tggatatgcc gcaggcggcg ggtatcgaac cgctgtaaga aatccgcctg
    14701 gcgttgtgac gcaacctcgc cgtacgactt gtactgtctg cgctcgcatt cctggccaga
    14761 cattcgactg cagacacggc aaggtcagac aagcaggcga ggcagcttgt gtgtgtgcga
    14821 tgccaaggct tcacaccggc agacgccggg cgttcctgtg ctgggctgtt ttcagcaagg
    14881 agcgtgcatg ttgtccgaac cccattcgtt tttggcgcag gcctttgccg gcccgcgcag
    14941 ctatctgccg atggccagcc atgcctacgc cctgtcgggc aatccgctga ccatggacga
    15001 ttttcatcgc cgttatctgc tgctcaacca gtatctggcc gacccgcatc cgctgaccca
    15061 gctcaagccc gaggccattg ccccgggcct ggccacgctg acctgtcagc aggtgattga
    15121 gctgctgctg cactggccgg gcgcgccgca gcgcaatgcc attctggacg cggtgtgtgt
    15181 cgagtttggt ctgtgtccgg tggataacag cccgtttcgc atctgggggt attacgcccc
    15241 aggtcagggg gcgttgccgc tgcatgtgta tatcggctgc gacggctggc tgtacgacac
    15301 cttgcccggc ctgcccgcac accgcacccg cgacagccat ggccgctgcc cgccaagctg
    15361 gttgttgcgc cacggcatgc agccgctgga tgaggcacac gtgtgcagca cagccgtgca
    15421 tgccctggcc gctggcaccc tggccagcat tcatgcgccg gagcaggcct ggcgcacggt
    15481 gacagaagcc agcggataag
//
LOCUS       DAOPYX010000030        15383 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_4139_length_15383_cov_8.903053, whole genome shotgun sequence.
ACCESSION   DAOPYX010000030 DAOPYX010000000
VERSION     DAOPYX010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 15383)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15383)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15383
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4139_length_15383_cov_8.903053"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(284..1054)
                     /locus_tag="PKN55_02810"
     CDS             complement(284..1054)
                     /locus_tag="PKN55_02810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX70208.1"
                     /translation="MGHGHGGKGDAGGKGAAAILTPVALTTGADSVGTSGNDWITATA
                     ATLNSGDSLVGGDGFDTISLSGSGVFNFSTLASFSGFERIQGGSADQTIILKDGLSLT
                     VDLGSGHNTLTAASGNTLVVTHGGTDTITGGSGSLTVRAEAGVSTIIAGSGGSHVDVG
                     YGTVNLTAGSGVDVIDIGHSSGQATVSGFAQGADKIDVHELHVSSYAELQAVASITSS
                     GGNTTITVTGGDTITLVGVSSVTESDFVFSSTSSAMHC"
     gene            1464..2498
                     /locus_tag="PKN55_02815"
     CDS             1464..2498
                     /locus_tag="PKN55_02815"
                     /inference="COORDINATES: protein motif:HMM:NF019379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1615 domain-containing protein"
                     /protein_id="HNX70209.1"
                     /translation="MPPFPAYPNEAAARALVQNKLLPASVRDKAGWSQEIVGAFTGLH
                     LPYRADLLCAAMAVIEQESTWQADPVVPGLGEMVWKKIAEKAGGVIPVAALKVAFLKP
                     SATGESYTSRIDRLRTEKEMNALFEDMVADARRLGLPVNMKNPIRTGGPMQVSVEFAE
                     EHVRAKGYPYPRKGSWRDEVFSRRGGLYFGIANLLDFPADYPDMRYRFADFNAGRYSS
                     RNAAFQAVVSRLSGMKLALDGDLLIYRDGAPSSSISSTQLALERLEGRLGMSRAEVSR
                     DLRLEKTPAFSQSALYQKVFALAGGHAARAVMPQIQLVSPKITRKLTTEWFADRVDGR
                     YKACLAREAR"
     gene            complement(2554..3669)
                     /gene="tgt"
                     /locus_tag="PKN55_02820"
     CDS             complement(2554..3669)
                     /gene="tgt"
                     /locus_tag="PKN55_02820"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HNX70210.1"
                     /translation="MLQFTLHSTSAGARRGTLTLNHGQIETPTFMPVGTYGSVKAMSP
                     LELNEIGAQIILGNTFHLWLRPGLDVIRQFGGLHQFIGWDKPILTDSGGFQVFSLGDL
                     RKITEEGVTFQSPINGDKLFLSPEKSMEIQHVLNSDIVMIFDECTPYPADEKTAADSM
                     RMSLRWAERSRKAFDDLHNPNALFGIVQGSMYPALREESLRELMAIGFDGIAIGGLSV
                     GEPKHEMQRMLQVMQPMLPAEKPHYLMGVGTPEDLVYGVAHGIDMFDCVMPTRNARNG
                     WIFTRFGDVKIKNAKYRSDTRPLDETCGCYACQNFSRAYLHHLHRAGEILGARLNTIH
                     NLYYYQQLMADMRAAIEADRFNDFVAQFHADRQRGVD"
     gene            3787..3863
                     /locus_tag="PKN55_02825"
     tRNA            3787..3863
                     /locus_tag="PKN55_02825"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3821..3823,aa:Val,seq:gac)
     gene            4070..5974
                     /gene="thrS"
                     /locus_tag="PKN55_02830"
     CDS             4070..5974
                     /gene="thrS"
                     /locus_tag="PKN55_02830"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HNX70211.1"
                     /translation="MPDIRLPDGSIRSFEQPVTVAGVAASIGAGLARATLAGKVDGKL
                     VDASHLIEHNADLVIVTERDADGLDIIRHSSAHLLAHAVKTLFPDAQVTIGPTIENGF
                     YYDFSYKRPFTPDDLAAIEKKMAELAKADIPVERYELPRDEAIAYFKSIGEAYKAEII
                     ESIPAGEVLSLYRQGDFTDLCRGPHVPSTGKIKVFKLMKLAGAYWRGNSKNEMLQRVY
                     GTAFMKKEELEAYLHMLEEAEKRDHRKLGQQLDLFHMQDEAPGSVFWHPKGWRLFQSL
                     INYMRQRQEQAGYVEVNTPDVMDRGLWETSGHWFNYRENMFTTQTEDERVFALKPMNC
                     PGAVSMYAHGLHSYRDLPMRMAEFGKVHRYEPSGALHGLLRVRHFTQDDAHIFCTEAQ
                     MEQECRDVVALILDIYKDFGFDNVRIKLSTRPENRIGSDEIWDKLEGALSSALESMNM
                     PYELFPGEGAFYGPKLEFVLRDTLGRDWQCGTLQVDMNLPERFDISYVAEDNSRKRPV
                     MLHRALFGSLERFTGILIEHYAGAFPTWLAPQQAVVMTITEAQCDYARDVVAQLRAAG
                     VRVEADLRNEKIGYKIREHSLQKLPYQIIIGDKEKAGGLVAVRTRTGEDLGQLTLSQF
                     IERIKSETLA"
     gene            6023..6541
                     /gene="infC"
                     /locus_tag="PKN55_02835"
     CDS             6023..6541
                     /gene="infC"
                     /locus_tag="PKN55_02835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012607480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HNX70212.1"
                     /translation="MAQEKEPRINGEITAREIRLVGSEGEQLGIVTLRDALERAEEAE
                     LDLVEIAPTAQPPVCKLMDYGKFKYQESKKKHEAKLKQKQIQVKEVKFRPGTDDGDYN
                     VKLRNLIRFLTDGDKAKITLRFRGREMAHQDIGLALLKRVEADLIEVGTVEQFPKLEG
                     RQMVMMIGPKKK"
     gene            6709..6906
                     /gene="rpmI"
                     /locus_tag="PKN55_02840"
     CDS             6709..6906
                     /gene="rpmI"
                     /locus_tag="PKN55_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012698199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HNX70213.1"
                     /translation="MPKMKSKSGAKKRLKVLGNGGVKRSMAFKRHILTKKTTKTKRQL
                     RGTVMVHSTNMAAVRAMLPYA"
     gene            6917..7276
                     /gene="rplT"
                     /locus_tag="PKN55_02845"
     CDS             6917..7276
                     /gene="rplT"
                     /locus_tag="PKN55_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004192938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HNX70214.1"
                     /translation="MPRVKRGVTARARHKKILALAKGYRGRRKNVYRIAKQAVMKAGQ
                     YAYRDRRQRKRQFRTLWIARINAAARELGLSYSKFMNGVKKAAIEIDRKVLADLAVFD
                     KPAFAKIVEQAKASLAA"
     gene            7543..8466
                     /gene="pheS"
                     /locus_tag="PKN55_02850"
     CDS             7543..8466
                     /gene="pheS"
                     /locus_tag="PKN55_02850"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011134906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HNX70215.1"
                     /translation="MVGLEQVKARYLGKSGELTELLKQLGKLPPEERKTAGAAINETK
                     QAFEAAFNARRDALAAEKLAAQLAAESLDVTLPGRGAAVGGLHPVTLTLERIAGLFHS
                     LGFDVADGPEIESDFHNFQALNIPANHPARAMQDTFYVEGGDVLRTHTSPIQIRYMLN
                     NPPPIKIIAPGRVYRVDSDATHSPMFHQMEGLWVDERVSFADLKATLTDFLRRFFERD
                     DLQVRFRPSFFPFTEPSAEIDVLGERGWLEVGGCGMVHPNVLKNVNIDPERYTGFAFG
                     IGLDRFAMLRYGVNDLRLFFENDLNFLKQFN"
     gene            8486..10843
                     /gene="pheT"
                     /locus_tag="PKN55_02855"
     CDS             8486..10843
                     /gene="pheT"
                     /locus_tag="PKN55_02855"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014086536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HNX70216.1"
                     /translation="MKFSENWLKSWVNPGLSTEQLNYLLTMAGLEVESCEPAAPAFSG
                     VVIAEVKTVRKHENADRLRVTEVDVGTGELVQIVCGAPNVAEGVKVPCALPGAVLPGD
                     FKIKPTKMRGVPSNGMLCSGKELGVPDEVDGLLLLPADAPVGASIRDYLDLDDHSIEL
                     KITPNRADCLGMRGIAREVAALTGAPLTAPALPAVAVTLDERRAVTLRADAACGRYLG
                     RVIRGVNQAAPTPDWMKTRLARAGLRSISAIVDVTNYILLEQGQPLHAFDLASLQGGI
                     VVRMAEQGETLTLLNGKEQALDADMLVIADEHKAVALAGVMGGANSEVSDSTQDVFLE
                     SAYFAPEAIAGRARRLGFSSDASFRYERGVDFTLQAEAMERATALILDICGGQAGPVT
                     EAVGQLPARAPVKVRVARVNQVLGLQLSADDMAAMFARLGFVAQADGEGFLVTPPSFR
                     FDIAIEEDLIEEVARLHGYDNIPSDAPQARARMLAQPETLTPRGELRRRVAARDYQEI
                     VSYAFVDSAWEQDFAANAAPIKLINPIASQMSVMRSTLFGGLVDTLVSNLNRKQARVR
                     LFEIARVFLKRGEAVEQPERVAGLAYGGRLPEQWGAKSERVDFYDVKADVEALLAPRV
                     AEFRKASHPALHPGRTADVLLDGVVIGVLGELHPQWVQKYDLPAAPVLFELDFAALLA
                     RQPVAAQPVSRLQPVRRDFAFVVDESVEAGALLAAFEALNLPAVTGIGLFDVYRGKGL
                     EEGKKSLAFKVLMQDTAKTLTDADVEPVVQAVVACAAQHGAHLRL"
     gene            11033..11335
                     /locus_tag="PKN55_02860"
     CDS             11033..11335
                     /locus_tag="PKN55_02860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002641098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HNX70217.1"
                     /translation="MTLTKAELADLLFDKVGLNKREAKDMVESFFEEIRVALEEGDAV
                     KLSGFGNFQLRDKPQRPGRNPKTGEEIPITARRVVTFHASQKLKGMVDDHYASKRA"
     gene            11307..11636
                     /locus_tag="PKN55_02865"
     CDS             11307..11636
                     /locus_tag="PKN55_02865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HNX70218.1"
                     /translation="MTTTRPSAPDLPPVPGKRYFTISEVSELTGLTPELLRQWELASA
                     PPSLAAQARPRRHYQHHEVLLVRRMRNALLASRPATTPAPATTNGRLGASSLRAELAS
                     ILAWLDI"
     gene            11695..11771
                     /locus_tag="PKN55_02870"
     tRNA            11695..11771
                     /locus_tag="PKN55_02870"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11729..11731,aa:Pro,seq:ggg)
     gene            12102..12344
                     /locus_tag="PKN55_02875"
     CDS             12102..12344
                     /locus_tag="PKN55_02875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX70219.1"
                     /translation="MLAITAAPSRNTVINRVSVWHDYLLCWRCWLLFLSQKSQNPSLS
                     LSGHALLTWIKAAHNTFLVLWCDLAILGVLLLWSYA"
     gene            12671..13000
                     /locus_tag="PKN55_02880"
     CDS             12671..13000
                     /locus_tag="PKN55_02880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H-NS histone family protein"
                     /protein_id="HNX70220.1"
                     /translation="MVDFQTFTFQELLQLKVELEVELKKREQEEKAAARKKILEIART
                     FNFDLDELFTSPKAGAGAPRRVVEPKYINPKDPEQTWTGRGRQPVWVQEHQANGGTLE
                     ELLINKA"
     gene            13207..13755
                     /locus_tag="PKN55_02885"
     CDS             13207..13755
                     /locus_tag="PKN55_02885"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNX70221.1"
                     /translation="MLALLCQYARCARDARLPMCAVRALGPIRYFQELLKMEFSAPHD
                     PSLRVLSLSIPAHRRHLPLLGAAVEAAAREAGASADAAYAINMAVVQAISAVLRYAGT
                     PALAQFELECLDSPDRIQVCIHDQGPPWVGTQALPGQADTLQAFELVRAMVDALEYLP
                     HPSGNRLKLTRFRTRPVLDDED"
     gene            complement(14146..15297)
                     /locus_tag="PKN55_02890"
     CDS             complement(14146..15297)
                     /locus_tag="PKN55_02890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012697768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HNX70222.1"
                     /translation="MAVAHRLDHIAPFHVMAILEKAKALEAAGHDVIHMEIGEPDFPT
                     PAPIVAAGMAALQNGRTFYTPALGIPELRQAIAHFYQQRYGVDVPARRIVVTPGASGA
                     LQLALAALVNPGEQVLLPDPTYPCNRHLVSLVNGEPVSIPVNADSGYQLQAEDIARHW
                     SANTVAAMIASPANPTGTLIAPDALQQLHQAVRAQGGVLLVDELYHGLTYDGDAHTAL
                     AVSDDIFVVNSFSKYFQMTGWRLGWLVVPDAYVDAVTRLAQNLFLSPSTPAQYAALAA
                     FTPETQRILESRRAEFQRRRDALLAAFAPLGFKFPAQPQGAFYVYADVSAHTDDSFAW
                     AEQILQDAHVAIAPGRDFGQHQAARHVRLAYTTSEARLREAAARIAACL"
BASE COUNT         3044 a         4671 c         4648 g         3020 t
ORIGIN      
        1 tgggcttggc gcgcggctct cgccatagcg ctgcgcataa catgcttttg cctggcggta
       61 ctgtttgcgg ccacatgctt ggcctgcagc gctttgctgc gtgttgtatc ggcccatcct
      121 gccggggcgg gcggccgcac acagtacggc tgtcgggcaa gcgcgccagg aggggtttgt
      181 gagccgcgct tagtgccccc ccaggcgttg ttgtgtgacc ttgtcgtacg atttgtactg
      241 tctgcggtca cacgcctggc caggatcgct tcgctgaatt ttgttagcag tgcatggcgc
      301 tgctggtgct gctgaacaca aaatcgcttt ctgtcaccga gctcaccccc accagggtga
      361 tggtgtcgcc acccgttacc gtgatggtgg tgttgccgcc gctgctggtg atcgacgcca
      421 cggcttgcag ttcggcatag ctggacacat gcagctcgtg cacgtcgatt ttgtcggcgc
      481 cctgggcaaa gccgctgacg gtggcctggc cgctgctgtg cccgatgtcg atgacgtcca
      541 cgccgctgcc agcggtgagg ttgaccgtgc catagccgac atcgacatgg ctgccgccgc
      601 tgccggcgat aatggtggac acacccgctt cggcgcgcac ggtcaggctg ccgctgccac
      661 ccgtgatggt gtcggtgccg ccgtgagtga ccaccagggt gttgccgctg gcagcggtga
      721 gggtgttatg gccgctgccc aggtctacgg tcaggctcag gccatccttg agaataatcg
      781 tctggtccgc gctgccgcct tgaatgcgct caaagccgga aaagctggcc agggtgctga
      841 aattgaaaac accgctgccg ctcaggctga tggtgtcgaa tccatcgccg ccaaccaggc
      901 tgtcaccact attcagcgtg gctgccgtgg cggtaatcca atcattgccg ctggtgccca
      961 cactgtcggc gccggttgtc agcgccacgg gtgtcaagat cgccgctgcg cctttgccgc
     1021 ctgcatcgcc cttgccgccg tgtccgtgtc ccatgatgat cctcctgaaa aatcaggcag
     1081 ttctcgctgc cttcatgact gttgcgtgat gagataaatg atacgaatcc cattgccacc
     1141 cgtcagccca tgacatcgta aaactcggta aacctgcgtt agcacagtgc aaaaccgctc
     1201 aagttatcgt cgtcgcggtg tggtggcggt ggccaaagcc gggctttgct acaatatcgg
     1261 ccgtgtatat tggctggtgc aaagcttgtg atgaaacctt ctctgtttgc cgccgcgacg
     1321 ctggcggccc tgctcagtgc ctgcgccacg gcaccccagc aaaccactcc tgttgttacc
     1381 cccagcgtgc ccagcatgcc cccaccccga tcaccacgcc aaccgggccc gtggtgtcgc
     1441 agccggtcag cccgccggcg ccggtgccgc catttcccgc ctatcccaat gaagccgctg
     1501 cccgtgccct ggtacaaaac aagttgctgc cggccagtgt gcgcgacaag gcgggctggt
     1561 cgcaggagat tgtcggggcg tttaccggcc tgcatctgcc gtatcgggcc gatttgctgt
     1621 gcgctgccat ggcggtgatc gagcaggaat ccacctggca ggccgatccg gtggtgccgg
     1681 gcttgggtga gatggtgtgg aagaaaatcg ccgaaaaagc cggcggggtg attccggtgg
     1741 cggcgttgaa ggtggccttc ctcaagcctt cggccaccgg cgagagttac acctcgcgca
     1801 tcgaccgact gcgcactgaa aaagaaatga acgccctgtt tgaagacatg gtggccgacg
     1861 cgcgccggct gggcttgccg gtcaatatga aaaacccgat ccgcactggc gggccgatgc
     1921 aggtcagtgt ggagtttgcc gaagaacatg tgcgcgccaa gggctaccct tatccgcgca
     1981 agggcagctg gcgtgatgag gtgttcagcc ggcgcggcgg gctgtatttc ggcattgcca
     2041 atttgctgga tttccctgcg gattacccgg atatgcgcta ccgctttgct gatttcaatg
     2101 ccgggcgcta cagcagccgc aatgccgcgt ttcaggctgt tgtgtcgcga ttgtcgggta
     2161 tgaagttggc gctggatggg gatttgctga tttatcgcga tggcgcaccc agcagcagca
     2221 tcagcagcac gcaactggcg ctggaacggc tggaggggcg cttgggcatg agtcgggccg
     2281 aggtgagccg ggatctgcgg ctggagaaaa ccccagcctt cagtcagagc gcgctgtatc
     2341 agaaggtgtt tgccctggct ggcggccatg ccgcgcgggc ggtgatgccg caaatccagc
     2401 tggtcagccc gaaaatcacc cgcaagctga ccactgaatg gtttgccgac cgggtggatg
     2461 gccgttacaa ggcgtgtctg gcgcgcgagg cgcgttgatg cgcggctgcc tcactgtgca
     2521 ctggcgcagt gaggcagcac gctggcgtgg cggtcaatcc acgccgcgct ggcggtcggc
     2581 gtggaattgc gcaacaaagt cgttaaagcg gtcggcctcg atggcggcgc gcatatcggc
     2641 catcagttgc tggtagtaat acaggttgtg gatggtgttc aagcgtgcgc ccaggatttc
     2701 gccggcgcgg tgcaggtggt gcaggtaggc gcggctgaaa ttttggcagg cgtagcagcc
     2761 gcaggtttcg tccagcggac gggtgtcgct gcggtatttg gcgtttttga tcttcacatc
     2821 gccaaagcgg gtgaaaatcc agccattgcg ggcgttgcgg gtgggcatca cgcagtcgaa
     2881 catatcaatg ccgtgcgcca cgccgtacac caggtcttcc ggcgtgccca cgcccatcag
     2941 atagtgcggc ttctcggccg gcagcatcgg ctgcatcact tgcagcatgc gctgcatctc
     3001 gtgcttgggc tcgcccaccg acaaaccgcc aatggcgatg ccgtcaaagc cgatggccat
     3061 cagctcgcgc agcgattctt cgcgcagcgc cgggtacatg ctgccctgca cgatgccaaa
     3121 cagcgcattc gggttgtgca gatcgtcaaa cgccttgcgg ctgcgctcgg cccagcgcag
     3181 gctcatgcgc atcgagtcgg cggcggtttt ttcgtcggcc gggtagggcg tgcattcgtc
     3241 aaaaatcatc acgatgtcgg aattcagcac atgctggatt tccatggact tctccggcga
     3301 cagaaacagc ttgtcgccgt tgatcgggct ttggaaggtc acgccttctt cggtgatttt
     3361 gcgcaaatcg cccaggctga acacctgaaa gccgcccgag tcggtgagga tgggtttgtc
     3421 ccagccaata aactggtgca ggccgccaaa ctggcggatg acgtccagcc ccgggcgcag
     3481 ccacaggtga aaggtgttgc ccaggataat ctgggcgccg atttcgttca gttccagcgg
     3541 gctcatcgcc ttgaccgagc catacgtgcc caccggcatg aacgtcggcg tttcgatctg
     3601 gccgtggttg agggtcagcg tgccgcgacg ggcgccggcg ctggtggaat gtaaggtgaa
     3661 ctgcaacatg gtgtcggtca agtttccaga aaaatcagac ggctagtata gcgcatgaag
     3721 aattttgaaa aaaatgcttg cacaagtgaa agtccatcta tacaatgcgc agctcctggt
     3781 aacgacaggc acgtagctca gctggttaga gcaccacctt gacatggtgg gggtcgttgg
     3841 ttcgagtcca atcgtgccta ccaatattat tgcggagtaa tcccatggct ttccgaacta
     3901 ctactaccgg catcagcacc ctcgcttgag cctcggaaag ttattgcgaa ttactgcgta
     3961 aacaccaaaa aagtgcggct cttgccgcac tttttttttg cccgttcgtt ttgccccatc
     4021 ctcagccgtc ccgtaaacgg gtctggctga tacagcccgg agaacagaca tgcctgacat
     4081 tcgattgccc gatggttcca tccgctcctt tgaacagccg gtcaccgtgg caggcgtggc
     4141 cgcctcgatt ggcgccggcc tggcccgcgc caccctggcc ggcaaagtgg acggcaagct
     4201 ggtggacgcc agccatctga ttgaacacaa cgccgacctg gtgattgtca ccgaacgcga
     4261 cgccgacggc ctggacatca ttcgccactc cagcgcccac ttgctggcgc acgcagtcaa
     4321 gacgctgttc cccgatgcgc aagtgaccat cggcccgacc atcgaaaacg gcttttacta
     4381 cgatttcagc tacaagcgcc cgttcacccc ggacgatctg gccgccatcg aaaagaaaat
     4441 ggccgagctg gccaaggcgg atattccggt cgagcgctac gagctgccgc gcgatgaagc
     4501 cattgcttac ttcaagtcga ttggcgaagc ctacaaggcc gagatcatcg aatccatccc
     4561 ggcgggcgaa gtgctcagcc tgtaccgtca gggtgacttc accgacctct gtcgcggccc
     4621 gcacgtgccg tccaccggca agatcaaggt gttcaagctg atgaagctgg ccggcgctta
     4681 ctggcgcggc aacagcaaga acgaaatgct gcaacgcgtg tacggcacgg ctttcatgaa
     4741 aaaagaagag ctggaagcct acctgcacat gctggaagaa gcagaaaagc gcgaccaccg
     4801 caagctgggt cagcagctgg atctgttcca tatgcaggac gaagcgccgg gctcggtgtt
     4861 ctggcacccc aagggctggc gtttgttcca gagcctgatc aactacatgc gccagcgtca
     4921 ggaacaagcc ggttatgtgg aagtgaacac gccggacgtg atggaccgtg gtctgtggga
     4981 aacctccggc cactggttca actaccgcga aaacatgttc accacgcaaa ccgaagacga
     5041 acgcgtgttc gccctcaagc cgatgaactg tccgggcgcg gtgtcgatgt acgcccacgg
     5101 cctgcacagc taccgcgacc tgccgatgcg catggccgaa ttcggcaagg tgcaccgcta
     5161 cgagccgtcc ggcgcgctgc atggtttgtt gcgtgtgcgc cactttaccc aggatgacgc
     5221 gcatatcttc tgcaccgaag cgcaaatgga gcaggaatgc cgtgatgtgg tggcgctgat
     5281 tctggacatt tacaaagact ttggctttga caatgtccgc atcaagctct ctacccgccc
     5341 ggaaaaccgc atcggctcgg acgaaatctg ggataagctg gaaggcgcgc tgtcgtcggc
     5401 gctggagtcg atgaacatgc cttacgagct gttcccgggc gaaggcgctt tctacggccc
     5461 caagctggag ttcgtgctgc gcgacaccct gggccgcgac tggcagtgcg gcaccttgca
     5521 ggtggacatg aacctgcccg agcgtttcga catcagctat gtggccgaag acaatagccg
     5581 caaacgtccg gtgatgctgc accgcgcgct gtttggttcg ctggagcgct ttaccggcat
     5641 cctgatcgaa cactacgccg gcgccttccc gacctggctg gcgccgcagc aggcggtggt
     5701 gatgaccatc accgaagcgc agtgcgatta tgcccgcgat gtggtggcac aattgcgcgc
     5761 cgcaggcgtt cgtgttgaag ccgacttgag aaatgagaaa attggctata aaatccgcga
     5821 gcatagcctg caaaaattgc cgtaccagat tatcattggc gacaaagaga aagcaggcgg
     5881 tctggttgcc gtgcgcacgc ggactggcga ggatcttggc cagttgacgc tttcccagtt
     5941 tatcgaacgg atcaagtccg aaaccttggc ctgaaactga ccacggcgca tgacattttt
     6001 gtaataatta ggagaatttg ctatagctca ggaaaaagaa ccccggatca acggtgaaat
     6061 caccgcacgt gaaatccggc tggtaggcag tgaaggcgaa caactcggca tcgttaccct
     6121 gcgcgacgcg ctggagcgcg ccgaggaagc cgaacttgat ctggtcgaaa tcgcacccac
     6181 cgcgcagccg ccggtgtgca agctgatgga ctacggcaag ttcaagtacc aggaaagcaa
     6241 gaaaaaacac gaagccaaac tgaagcaaaa gcaaattcag gtcaaggaag tcaaattccg
     6301 ccccggcacg gatgacggtg actacaacgt caagctgcgc aacctgatcc gcttcctgac
     6361 ggatggcgac aaggccaaga tcaccctgcg cttccgtggg cgcgaaatgg cccaccagga
     6421 catcggcctg gcgctgctca agcgcgtcga ggcggatctg atcgaagtcg gcaccgtcga
     6481 gcagtttccc aagctcgaag gccggcagat ggtcatgatg attggcccca agaagaaata
     6541 aggctatcat atgcagttca tcggcacggc agggtgacct gccgtgttga tttttgcgaa
     6601 accgtgcaag cggcttcgca caaaagcgtg gcgttgcggt tctcaaagca cagtggggtt
     6661 tcctgctgtc acctctcgcc aaatctaaca aatcagcagg agcaatccat gcctaagatg
     6721 aaaagcaagt cgggcgcgaa aaagcgtctg aaagtgttgg gcaacggtgg tgttaagcgc
     6781 tcgatggcat tcaagcgcca catcctcacc aagaagacca ccaagaccaa gcgccaactg
     6841 cgcggcaccg tgatggtcca cagcaccaat atggctgctg ttcgtgcaat gctgccctac
     6901 gcttaaggag taaactatgc ctcgcgtcaa acgtggcgtt accgcccgtg cccgtcacaa
     6961 gaaaatcctc gcgcttgcca aaggctatcg cggtcgtcgc aaaaacgtat accggatcgc
     7021 caaacaggcg gtgatgaagg ccggtcaata cgcttaccgt gaccgtcgtc aacgcaaacg
     7081 ccagttccgc accctgtgga tcgcccgtat caacgccgcc gcgcgtgaac tcggtctgtc
     7141 ctacagcaag ttcatgaacg gcgtgaagaa ggctgccatc gagatcgacc gcaaggtgtt
     7201 ggccgatctg gcagtgttcg acaagccggc attcgccaaa atcgtcgagc aagccaaggc
     7261 cagcctcgcc gcttaagcaa gtgcgttgaa aaagggaggc cccgagcctc ccttttttta
     7321 tcacccgggt ttttatgctg cgcacaaaac ctggcgtgcg gcgcgccgcc tgaccacgcg
     7381 ctcagcccgt tcaccacgcc cgatgccgcc atgccatgtc ctggcccgcg tcgggcgcgg
     7441 taaaccgact gaaccgaccg cattcaccca aagaccgcaa tcatgaccca actcgcaacg
     7501 attcttgcca cggcccaggc agccttggcc gacgtgaccg acatggtcgg gctcgaacag
     7561 gtcaaggccc gctaccttgg caaatccggc gagctgaccg agctgctcaa gcagctgggc
     7621 aagctgccgc ctgaagagcg caaaaccgcc ggcgccgcca tcaacgaaac caagcaggcg
     7681 tttgaagccg ccttcaacgc ccgccgcgac gcgctggccg ccgaaaaact ggccgcccag
     7741 ctggccgctg aatcgctgga cgtcaccctg cccggccgcg gcgccgccgt gggtggcctg
     7801 cacccggtga cgctgacgct ggagcgcatt gccgggctgt ttcattcgct gggctttgac
     7861 gtggccgacg gcccggaaat cgaatcggat tttcataatt ttcaggcgct gaatattccg
     7921 gccaaccacc cggcgcgcgc catgcaagac accttctacg tggaaggcgg cgacgtgctg
     7981 cgcacgcaca cctcgccgat ccagatccgc tacatgctca acaacccgcc gccgatcaag
     8041 atcatcgcgc cgggccgggt gtatcgcgtg gattccgacg ccacccattc gccgatgttc
     8101 caccagatgg aaggcctgtg ggtggacgag cgcgtcagct ttgccgacct gaaagccacg
     8161 ctgaccgact tcttgcgccg ctttttcgag cgcgacgacc tgcaagtgcg tttccgcccg
     8221 tcgttcttcc cgtttaccga accgtcggca gaaatcgacg tgctgggcga gcgcggctgg
     8281 ctggaagtgg gcggctgcgg catggtgcac cccaatgtgc tgaaaaacgt caatatcgac
     8341 ccggaacgct acaccggctt tgccttcggc attggtctgg atcgcttcgc catgctgcgc
     8401 tatggcgtca acgacctgcg tctctttttt gagaacgacc tgaacttcct caagcaattc
     8461 aactgaccgc aggcgaggct caccgatgaa attctctgaa aactggctga aaagctgggt
     8521 gaaccccggc ttgtccaccg aacaactgaa ctacctgctg accatggccg ggctggaagt
     8581 ggaaagctgc gagcccgctg cgccggcgtt ttccggcgtg gtgattgctg aagtcaaaac
     8641 cgtgcgcaag cacgaaaacg ccgaccgtct gcgcgtcacc gaagtggacg tgggcacggg
     8701 cgagctggtg caaatcgtct gtggcgcgcc caatgtcgcc gaaggcgtga aagtgccgtg
     8761 cgcgctgccg ggcgccgtgc tgccgggcga cttcaagatc aagccgacca agatgcgcgg
     8821 cgtgccgtcc aacggcatgc tgtgctccgg caaggaactg ggcgtgcccg atgaggtgga
     8881 cggcctgttg ctgctgccgg ccgatgcgcc ggtgggcgcc tcgatccgcg attatctgga
     8941 tctggacgac cacagcatcg agctgaaaat cacccccaac cgcgccgact gcctgggcat
     9001 gcgcggcatc gcccgcgaag tggctgcgct gaccggcgcg ccgctgactg cgccggcgct
     9061 gccggccgtg gcggtgacgc tggacgagcg ccgcgccgtc accctgcgcg ctgacgccgc
     9121 ctgcggccgt tatctgggtc gggtgattcg cggcgtcaac caggccgcgc cgacgccgga
     9181 ctggatgaaa acccgcctgg cccgcgccgg cctgcgctcg atttccgcca ttgtcgatgt
     9241 caccaattac atcctgctgg aacaaggcca gccgctgcac gcctttgatc tggccagcct
     9301 gcaaggcggc atcgtggtgc gcatggccga gcagggcgaa acgctgaccc tgctcaacgg
     9361 caaggaacag gcgctggacg ccgatatgct ggtgattgcc gacgagcaca aagccgtcgc
     9421 cctggctggg gtgatgggcg gcgccaatag cgaagtcagc gacagcaccc aggatgtgtt
     9481 tctggaatcc gcctactttg ccccggaagc cattgccggg cgcgcccgcc ggctgggttt
     9541 ctcctccgac gcctcgttcc gctacgagcg cggcgtggac ttcaccctgc aagccgaagc
     9601 catggagcgc gccaccgcgc tgattctgga catctgcggc ggccaggccg gcccggtgac
     9661 cgaggccgtg ggccagctgc cggcgcgcgc gccggtcaag gtgcgcgtgg cgcgcgtcaa
     9721 ccaggtgctg ggcttgcaac tgagcgcgga tgacatggcc gccatgttcg cccgcctggg
     9781 ttttgtcgcc caggccgacg gcgaaggctt tttggtgacg ccgcccagct tccgctttga
     9841 catcgccatc gaagaagacc tgatcgaaga agtcgcccgc ctgcacggct acgacaatat
     9901 tccgtccgac gccccgcaag cgcgcgcgcg catgctggcc cagccagaaa cgctgacccc
     9961 gcgcggcgaa ctgcgccgcc gcgtggccgc ccgcgactat caggaaatcg tcagctacgc
    10021 ctttgtggac agcgcctggg aacaggactt tgccgccaac gccgcgccga tcaagctgat
    10081 caacccgatt gccagccaga tgagcgtgat gcgctccacc ctgtttggcg gtctggtgga
    10141 caccctggtg agcaacctca accgcaagca agcgcgcgtg cgtctgtttg aaatcgcccg
    10201 cgtgttcctc aagcgcggcg aggcggtcga gcagcccgag cgcgtggccg gcctggccta
    10261 cggcggccgc ctgcccgagc aatggggcgc caagagcgag cgcgtggact tttacgacgt
    10321 caaggccgat gtggaagccc tgctggcccc gcgtgtggcg gagttccgca aggccagcca
    10381 cccggcgctg catccgggcc gcacggccga cgtgctgctg gacggcgtgg tgattggcgt
    10441 gctgggcgaa ttgcatccgc agtgggtgca aaagtacgac ctgccggccg cgccggtgct
    10501 gtttgagctg gattttgccg ccctgctggc ccgccagccg gtggccgccc agccggtgtc
    10561 gcgcttgcag ccggtgcgcc gcgactttgc ctttgtggtg gacgaaagcg tcgaggccgg
    10621 cgcgctgctg gcggcgtttg aggcgctgaa cctgccggcg gtgacgggca ttggcctctt
    10681 tgacgtctat cgcggcaaag gtctggaaga aggtaaaaag agccttgctt tcaaggtgtt
    10741 aatgcaggat actgcgaaaa cgctgaccga cgctgacgtc gagccggtgg tgcaggcggt
    10801 ggtggcctgc gccgcccagc atggcgccca tctgcgcctg taagcctccc caccttgtgt
    10861 aatgcaaagc tgctgccggc tattgggccg gcagcagctt gtttttccgc gcctgcctgg
    10921 gcaggcgcca accatgattt gactgtcgcc acatccggcg cgcgtctctg tcttttgctt
    10981 gttgcggggg cttttgatgg cgcgcggccg gcgttatgat taaggggtaa gcatgacact
    11041 gaccaaggct gaacttgccg atttgctatt cgacaaagtc ggcttgaaca aacgcgaagc
    11101 caaagatatg gtggagtcgt tttttgaaga aattcgcgtg gcgctggaag aaggcgatgc
    11161 cgtgaagctg tcgggctttg gcaatttcca gctgcgcgac aaaccgcagc gtccgggacg
    11221 caaccccaaa accggcgagg agattcccat caccgcgcgc cgggtggtca cgttccacgc
    11281 cagccaaaag ctgaagggaa tggtcgatga ccactacgcg tccaagcgcg cctgatctgc
    11341 cgccggtgcc cggcaaacgg tatttcacca tcagcgaagt cagcgagctg accgggctga
    11401 cgccggagtt actgcgccag tgggaactgg ccagtgcacc gcctagcctg gccgcgcaag
    11461 cccggccgcg tcggcattat cagcatcacg aggtgctgtt ggtgcgccgc atgcgcaatg
    11521 ccctgctggc cagccgaccg gcaacgacac ctgcgccggc cacgaccaat ggtcgtctgg
    11581 gcgccagttc gctgcgggct gaactggcct cgattctggc ctggctggat atctgattgt
    11641 cactgggggc tagcgcagca gaaattgtgc tgttataatt gcgctcttgt tgtacggagt
    11701 gtggcgcagc ctggtagcgc atctgcatgg ggtgcagagg gtcggtggtt cgaatccact
    11761 cactccgacc aaattcgaaa aagccgacag tttactgtcg gcttttttca tgtctgtatg
    11821 gtttgtcgtc gtatccatgg caatcctgga tcagaaccgc tcacaaaacc ctgctggcgt
    11881 tgttgcgcaa ccttgccgta cttcttactg tctgcggtcg tccacctagc gcaggatcgc
    11941 agcgccgggt tttgtgagcg acgctaagcc acattctggc cggcttgcca tcagcatcgg
    12001 gccgtgccat cagcggcagt ggcatgcgag ccaagcggta ttcacctggc agcgctttta
    12061 cagaaaatga actcttttcg actggcctgt ctaaggacct tatgctcgcc atcacggcgg
    12121 cgccatcgcg caataccgtg ataaaccgag tgagtgtgtg gcacgattat ctgctgtgct
    12181 ggcgctgctg gcttttgttt ctttctcaga aatcacaaaa tccatcgctt tccctgagcg
    12241 gccatgcttt gctgacctgg ataaaggccg ctcacaacac ttttctggtg ttgtggtgtg
    12301 atcttgccat ccttggtgta ctgcttctgt ggtcgtacgc ctaagcagaa ccgcagcgct
    12361 gggttttgtg agccgcgcta aatacgctgg cgcgtgtcac acaccgacca gccaggcaga
    12421 atccctgctg agggtagtta aatcatttag tctggccaga caggtagatt cacctattca
    12481 gatctgtttc ttcacgttat ttaacacgcc cattgccagt gtggctcaag gtctgaccag
    12541 gaagaatttt cctgcccgga attttacccg gcccgacatt aaataaaatg cttgacgaaa
    12601 ccatgatgaa ttggctaatt taatagggac attaaatgcc cccgacacac tgacccaagg
    12661 atattaaaca atggttgatt tccagacctt caccttccaa gaactgctgc aactgaaagt
    12721 cgaactcgaa gtcgagctga aaaagcgcga gcaggaagaa aaagccgccg cccgcaagaa
    12781 gattctcgaa atcgctcgca ctttcaactt tgatctggat gaactgttca cctcgcccaa
    12841 ggctggcgca ggtgctccgc gtcgcgtggt tgagcccaag tacatcaacc cgaaagatcc
    12901 ggaacaaacc tggactggcc gtggccgtca gccggtttgg gtgcaagagc accaagccaa
    12961 cggtggcacc ctggaagagc tgctgatcaa caaggcctga ttttccccgc cttgccgcca
    13021 cgcgccgctc cgtgttcacg gttgcggcgc gtggcgtttt attgtctgtc catttcctca
    13081 acatgcctct tctgcggtgc ggcttttgtc atgccggcaa gccagaagca tgccgaatgt
    13141 cgcttttgcg gcttgaatcg ccctgataac ccgctacact gaatgtatgt cattcattgt
    13201 gcgtaaatgt tggcgctgct ctgccaatat gcaagatgcg caagggatgc ccgtttgccg
    13261 atgtgcgccg tacgcgcgct cggaccaatc aggtactttc aggagctgtt gaagatggaa
    13321 ttttctgcgc cgcacgatcc ctccctgcgt gtgctgagct tgagcattcc ggcgcatcgt
    13381 cgccacttgc cgttattggg cgctgcagtt gaggctgccg cgcgtgaggc cggcgccagc
    13441 gcagatgccg cctatgccat caatatggcc gtggtgcagg ctatttctgc cgtgctgcgc
    13501 tatgccggta cgccggcgct ggcccagttc gagctggaat gcctggattc gcccgatcgc
    13561 atacaagtgt gcatccacga tcaagggccg ccctgggtgg gcacacaggc cctgccaggc
    13621 caggctgata ccttgcaggc gttcgaattg gtacgcgcca tggttgatgc gctggaatac
    13681 ttgccacatc ccagcggaaa ccggctaaag ctcactcgtt ttcgtacccg tcccgtgctg
    13741 gacgacgaag attaagcgtc gctcacaaaa ctcagcaaag cgcgcctggt cagccgtgcg
    13801 gctgcagacc gccgcgccac gacaccaaga gggtgttgtg agcggcgctg agcgccacgg
    13861 acaaaacatg ccgacgactt tgctatgcaa tggccacatg cttgagcaag atcgctttgc
    13921 tggattttgt cttcgaatct gctgagattc ttgccatgtg gttttgcatg ctgatgaggc
    13981 agcatgacat accagacata aaatgttgta ttcataccac agtttatggt tgtttcccat
    14041 gtaaccggca aacaataatc ttgccccggt gaactgatga aaaagcccgc gcagggccag
    14101 gcctgcgcgg gcttttttac catccgccaa tctgcttggt ggcttttaca gacacgctgc
    14161 gatacgtgcc gccgcctcgc gcaagcgcgc ctcagaggtg gtgtaagcca ggcgcacatg
    14221 gcgggcggcc tggtgctggc caaaatcgcg gcccggggca atcgccacat gggcatcctg
    14281 caaaatctgc tcggcccagg caaagctgtc gtcggtgtgc gccgagacat cggcatacac
    14341 ataaaatgcg ccttgcggct gcgccggaaa cttaaagccc agtggcgcaa atgctgctag
    14401 cagcgcgtcg cggcggcgct gaaattctgc ccggcggctt tccagaatgc gctgggtttc
    14461 cggagtaaat gccgccagcg ccgcatattg cgccggggtg gagggcgaca aaaacaaatt
    14521 ctgcgccagc cgggtcaccg catccacata ggcatcggga accaccagcc agcccagccg
    14581 ccagcccgtc atctggaaat acttggaaaa actgttcacc acaaaaatat catcactcac
    14641 cgccagcgcg gtgtgcgcat caccgtcata ggtcaggcca tgatacagct cgtccaccaa
    14701 taacaccccg ccttgcgcgc gcaccgcctg atgcaattgc tgcaatgcgt caggggcaat
    14761 cagcgtgccg gtcgggttgg ccgggctggc aatcatggcc gccacggtat tggccgacca
    14821 atggcgggca atatcttcgg cctgcaattg atagccgctg tcggcattca ccggaatact
    14881 caccggctcg ccattcacca ggctcaccag atgccggttg catgggtagg tggggtcggg
    14941 caataacacc tgctcgcccg gattcaccag cgccgccagc gccagttgca gcgcgccaga
    15001 cgcgcccggc gtcaccacaa tgcgccgcgc cggcacgtcc acgccatagc gctgctgata
    15061 aaagtgggca atcgcctggc gcaattccgg aatgcccagc gccggggtat aaaacgtgcg
    15121 gccgttctgc aaggcggcca tgccggcggc aacaatcggc gccggggtgg gaaagtccgg
    15181 ctcgccgatt tccatgtgaa tcacgtcatg cccggccgcc tccagcgcct tggctttttc
    15241 cagaatggcc atgacatgaa atggggcgat gtggtccaga cggtgggcga cagccataaa
    15301 acacctttgc aaaaagccga ttatacgccg gccaatcgcc gcgccaaccg ccagcggcaa
    15361 aatcctgggc ttaaattccg cat
//
LOCUS       DAOPYX010000031        15218 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_4223_length_15218_cov_10.612016, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000031 DAOPYX010000000
VERSION     DAOPYX010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 15218)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15218)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15218
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4223_length_15218_cov_10.612016"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1284)
                     /locus_tag="PKN55_02895"
     CDS             complement(<1..1284)
                     /locus_tag="PKN55_02895"
                     /inference="COORDINATES: protein motif:HMM:NF024589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNX70223.1"
                     /translation="MSEALQPNAPAGGFAAISKYNPHLWSAEQLRAIFVARQHELSEL
                     LDTLRTTPVDSVGQHTLLIGARGMGKSTLMQRLALAVDDDAELRTHWLALRFPEEQYT
                     VNTVGQFWANVLDSLIDALEQRGQPTQALDAQAQRLAALPPAEQAAACLDAINHCADQ
                     YQQRLLLLVDNTDLLLHNIGAAAHWSLRQVLQSNARLLWVGGSYQSLEADSHYHDAFL
                     DFFRNIELRPLRLEEMQQALLALAATFGGDSARHAMQSQLNAHPERLPTLRQLSGGNP
                     RTTVMFYELLAHSPHGDVRSDLEALLDAMTPLYKARMDALAELPRKLLAHVFEHWAPI
                     SLGQLADASHVAKSSISPQLQRLQLDGLIEKTPLHGTTRSGYQAAERFFNIWYLMRFS
                     PRRQRARLAWLVEFMRLWFSQDELCQMARQRLDALP"
     gene            complement(1277..2482)
                     /locus_tag="PKN55_02900"
     CDS             complement(1277..2482)
                     /locus_tag="PKN55_02900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX70224.1"
                     /translation="MMLGQPATGEDFCGRRQELDDLWRYLENEHIRFPGVRRLGKTSI
                     LKRLQEQAAEQGVLARWLDVSHIHSAESFVLALEQAYPPPALRKFANDQAQSIAGWFS
                     RLRRIDASLPEIIGGGGLGIELGDGPMPDWLVAGEVLRQRLQPQPLLILLDEFPVMLQ
                     TLLDTRPADAQQLLAWLRIWRQTGGPCRFVFTGSIGLQSLLERHHLADKMNDCYDYPL
                     GAFRPTEARAMWQKFTQQAGSSASDEVIENALTRLGWLSPYFLCLLLDETLRAARERQ
                     QENPTPADRDMLILSDVHDAYEKLLANRSRFYHWELRLKTTLPASELALCQDLLKHVS
                     RHADGLTLRQLHSRLSKHEADAERRTQRLQDLLVRLSDEGYLSPPSASQRVQFLSFPL
                     RDWWSRNHV"
     gene            complement(2554..3663)
                     /gene="rodA"
                     /locus_tag="PKN55_02905"
     CDS             complement(2554..3663)
                     /gene="rodA"
                     /locus_tag="PKN55_02905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012698515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HNX70225.1"
                     /translation="MNDYYLLRRAWERLRSPIDGWLLALIMALFALSLVVLFSASNQN
                     LDKVINKMTFMLVALGIMWGVANVRPQTLMQLAVPGYIVGVLMLLGVALFGEVVNGSR
                     RWLHVGVTRIQPSEVLKIMVPMMLAWYFQKHENALRWHHYLVGAVIIIIPSLFILKQP
                     DLGTATLIMASGFFVLFLAGLPWKVMITGAVAFGASLPFVWNMLHDYQRKRVLTLIDP
                     SSDPLGAGYHIIQSMIAIGSGGPTGKGWLSGTQTHLEFIPERTTDFIFAVYAEEFGLL
                     GNLILLALYLLVIGRGLMIAAKATTLFGRLMAGSITLSFFTYAFVNMGMVAGILPVVG
                     VPLPLISYGGTAMVTLLIGFGILMSIHKNRPLIKR"
     gene            complement(3660..>5198)
                     /gene="mrdA"
                     /locus_tag="PKN55_02910"
     CDS             complement(3660..>5198)
                     /gene="mrdA"
                     /locus_tag="PKN55_02910"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008954687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HNX70226.1"
                     /translation="SANIWRFPGVEVKARLFRDYPFKEITAHVIGYIGRINQKEMERL
                     EENDELADYRGSTHIGKTGLEYTYEKVLHGTAGFEEMETDAGGRPVRTLRRSSPINGH
                     TLKLALDIELQKFAWERFGERRGALVALDPATGGVLAFISKPGYDPNLFIDGIDSQSW
                     KDLNDDWKKPLVNRALRGLYPPGSTFKPFMAMAALETKFRDPNYTIPDPGYFSLPGST
                     HRFRDSKPSGWGSMSMFRSIQVSSDTYYYKLAWDMGIDRLEPQLAKFGFGSPTGIDLD
                     GEARGVLPSKEWKRKRFAGKRYPEAARVWTPADVVPIGIGQGYNTYTPLQVAHAVAIL
                     ANNGVVYRPHVVKEIENLKTGEITKVEPNPVRDNHPDPANIAFVKSAMEAVLKPGGTA
                     ARIGAGLSYRMAGKTGTAQVVQIKQGAKYNAAALAEQHRDHSWFVAFAPVEAPRIAIA
                     VIVENGGWGAAAAAPLAREVADFYLTGRKGEVSAPNSDAPSAQHVRADKNKPAAAPAE
                     EVVE"
     assembly_gap    5199..5298
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5299..5642)
                     /gene="mreD"
                     /locus_tag="PKN55_02915"
     CDS             complement(<5299..5642)
                     /gene="mreD"
                     /locus_tag="PKN55_02915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011137905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HNX70227.1"
                     /translation="MAFDRPKELLKPIKPRWIVLSFVLAIAVELLPLDATLANWLPDF
                     TAMLALYWTLNQPRRFGIGWSFGIGLIADVASASVFGQHALAYSVISYLLLSRQRQVV
                     MYNLGQQTLVVLG"
     gene            complement(5646..6548)
                     /gene="mreC"
                     /locus_tag="PKN55_02920"
     CDS             complement(5646..6548)
                     /gene="mreC"
                     /locus_tag="PKN55_02920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014085609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HNX70228.1"
                     /translation="MDIASSPRFFRRGPKPLTRLLACGALSLGLLVADAKLHLLDPAR
                     GALTALLYPMQWLVTAPPEGLRQLDTFLTTQATLTHENQALRTYQLELEARLQRQAAL
                     QRENAELRQTLSLRSQHGGNTLPAEIVYTSRDPFTFTLIIDKGSMDGVQAGLPVIDAR
                     GLIGQITRVQPLTAEVTLLIERNHPVPVMIQRNGLRAVMYGMGGQAEIRHIAAHADVQ
                     VGDMLVTSGIDGVYPAGLPVAKVAMVDRNGGSPFARIVCTPLAGVGENRTLLVLNETL
                     NLPPRPEAAPAPKTKSRRKRGSDE"
     gene            complement(6631..7671)
                     /locus_tag="PKN55_02925"
     CDS             complement(6631..7671)
                     /locus_tag="PKN55_02925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012698511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HNX70229.1"
                     /translation="MFRSITGYFSHDLAIDLGTANTLIYVTGKGIVLDEPSVVSIMHE
                     GASNKKSILAVGLEAKKMLGRTPGSIQAIRPMKDGVIADFTVTEQMLKQFIKKVSPSR
                     VFSSSPRIVICVPCGSTQVERRAIKESALGAGARRVELIEEPMAAAIGAGLPVEEATG
                     SMVVDIGGGTTEVGVISLGGIVYSNSVRVGGDKFDEAIINYIRRNYGMLIGETTAEEI
                     KKTIGSAFPGAEVREMEVKGRNLAEGIPRAFTVSSNEILEALTEPLNQIVSAVKIALE
                     QTPPELGADIAEKGMVLTGGGALLRDIDRLLQEETGLPVFVADDPLTCVVRGSGRALD
                     KMDKVGTLFTND"
     gene            7890..8177
                     /gene="gatC"
                     /locus_tag="PKN55_02930"
     CDS             7890..8177
                     /gene="gatC"
                     /locus_tag="PKN55_02930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004574467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HNX70230.1"
                     /translation="MSLSATDVSRIARLARLHMDESEIAAVQGQLNGIFGLIEAMQAV
                     DTTGVAPMSHPQDVSLRLRDDVVSESDRRAAYQAVAPSVENGLYLVPKVIE"
     gene            8227..9675
                     /gene="gatA"
                     /locus_tag="PKN55_02935"
     CDS             8227..9675
                     /gene="gatA"
                     /locus_tag="PKN55_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017507471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HNX70231.1"
                     /translation="MNYTLKQLSDALAAGQMSSRELTRDYLARIDALNPQLNAFITVD
                     HDGALAAAAAADAARAAGQAGALTGVPFAHKDVFVTEGLLTTCGSKMLGNFVGPYDAH
                     VVSQCRQAGLVMLGKTNMDEFAMGSSNENSAFGAVKNPWDVKAIPGGSSGGSAAAVAA
                     GLVPAATGTDTGGSIRQPASHCGITGIKPTYGTVSRYGMVAYASSLDQGGPMARTAED
                     CALLLNVMAGFDARDSTCLDRAKEDYTRSLNHPVAGLTIGLPKEFFAAGLSADVARVI
                     DAAVAQFKALGAKIVEVSLPKTELSIPAYYVIAPAEASSNLSRYDGARYGHRAAQYKD
                     LTDMYERSRAEGFGAEVKRRILVGTYVLSHGYYDAYYIQAQKIRRLIAEDFQAAFQQC
                     DFILGPVAPTAAFNLGEKSDDPVQMYLSDIYTLAVNLAGLPGMSLPAGFADNGRPVGV
                     QLIGNYFSEAAMLGAAHQFQLATDWHARTPAL"
     gene            9722..11155
                     /gene="gatB"
                     /locus_tag="PKN55_02940"
     CDS             9722..11155
                     /gene="gatB"
                     /locus_tag="PKN55_02940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012698508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HNX70232.1"
                     /translation="MKWEVVIGLEVHTQLSTQSKIFSGSSTAFGAAPNTQASAVDIAL
                     PGVLPVMNRAAVDKAIRFGLAIGAKINRTSIFARKNYFYPDLPKGYQISQFELPIVEG
                     GSLQIQVGDSEKTVNLTRAHLEEDAGKSLHEDFHGMTGIDLNRAGTPLLEIVSEPEMR
                     SAAEAVAYARALYTLVTWLGICDGNMQEGSFRVDANVSIRPFGQAEFGTRREIKNLNS
                     FRFLEQAINAEIRYQTELIEDGGKVQQATVLFDPDSGETRAMRSKEDAHDYRYFPDPD
                     LLPLKVSDAEVDAIRADMPELPGEMKQRFIDSFAVSAYDAGLLTQSLALARYFEAAAQ
                     ACPGQGKLVANWVNGEVAARLNREEKSIADCPLSAERLAGLVARVADNTLSNKLAKQV
                     FEALWDSELSADAVIERDGLKQVSDVGAIEKLVEEALAANPKAVEEFRAGKEKALNAL
                     AGQVMKASKGKANPAQVQDILRQKLAG"
     gene            complement(11225..12130)
                     /locus_tag="PKN55_02945"
     CDS             complement(11225..12130)
                     /locus_tag="PKN55_02945"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HNX70233.1"
                     /translation="MDARLPELRQLASLVEIIRHGSFTRAASALHQTQSALSKQIAQL
                     EARLGQPVLVRGPGQLRLTDAGEVALRRAEQMLAHGQAMLAELDDQTPQIRGALRLGL
                     PLFGADALFTQVIVEYLHRYPEVRVQLEEGGSKRIEQALLTGTLELGAALTPSHPELA
                     HTPFCHEPLDVLAPANHPCATAGAVTLAALADTPFLFYERSFTLNDRLWQACRQAGFT
                     PREGGRSGQADFLATLVAAGQGVVLLPRVVARGLARPGLVRLALSEPAMTWDLAFIWR
                     REGYLSRAARAWLALVQEMRQPVAD"
     gene            12233..12619
                     /locus_tag="PKN55_02950"
     CDS             12233..12619
                     /locus_tag="PKN55_02950"
                     /inference="COORDINATES: protein motif:HMM:NF015729.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HNX70234.1"
                     /translation="MSNAISSGLQALAQVTALLALWSLGKVLAAWWALPVPGGVIGLA
                     LLLLLFACRLVQPGWLRAGAGLLLAEMLLFFIPAVMSLLDYGPLLRAQGPRILLVIVS
                     GTLLVMMSTALAVEWVCRRHGHALED"
     gene            12631..13305
                     /locus_tag="PKN55_02955"
     CDS             12631..13305
                     /locus_tag="PKN55_02955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018747151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HNX70235.1"
                     /translation="MPLWSWPWLTLLAYALARSLHRRQRRFWLSPIVLAPLLLFALAI
                     PSHTHYADYAHDTGWLVSLLGPATVAFAVPIWTQRALLARHWLALGAGMLAGSAMALF
                     SSWQLAHALALDGQVARSLLPRSITTPFAMEMSRDLGGAPELTAAFVMVTGIVGMLAG
                     HLLLRLLPLKTALARGAMLGVAAHGAGAAKAHELGGPEGTVAGLLMVLTGIFNLLLAP
                     LLLKLL"
     gene            13444..14391
                     /gene="mmuM"
                     /locus_tag="PKN55_02960"
     CDS             13444..14391
                     /gene="mmuM"
                     /locus_tag="PKN55_02960"
                     /EC_number="2.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008953813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047150 - betaine-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase"
                     /protein_id="HNX70236.1"
                     /translation="MLSQTLQDFPLLILDGALATELERRGCDLRDALWSARVLLDNPE
                     LIRAVHHDYFAAGADVATTASYQASYEGFARRGLSAEQATACLQLSVQLAREARDAFW
                     AANPQLHGVRPRPLVAASVGPFGAMRADGSEYVGHYGVDDATLRDFHRPRLAALLAAG
                     PDVLACETLPCLQEACVLAELLRAEFPQAQAWVSFSCRDGAHTCQGEPLAEAVAALAD
                     CPQVVAVGVNCTAPGFIPELVAAARAASDKPVLVYPNAGETYDPVHKCWHGCADPQAF
                     AEAARHWHAQGARLIGGCCRTTPEDIRALAAWARPAATN"
     gene            15134..>15218
                     /locus_tag="PKN55_02965"
     CDS             15134..>15218
                     /locus_tag="PKN55_02965"
                     /inference="COORDINATES: protein motif:HMM:NF014439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HNX70237.1"
                     /translation="MFGGVSTLSLDSKGRLAIPARHRERLLA"
BASE COUNT         2689 a         4634 c         4931 g         2864 t
ORIGIN      
        1 gggcagcgca tccaggcgct gtctggccat ctggcacagc tcgtcctggc tgaaccacag
       61 gcgcatgaac tccaccagcc aggccaggcg ggcgcgttgg cggcggggag agaagcgcat
      121 caaataccag atattgaaaa accgctcggc agcttggtag ccgctgcggg tggtgccatg
      181 cagcggggtt ttttcgatca ggccgtccag ttgcaggcgc tgcaattgtg gcgagatgct
      241 gcttttggcc acgtgcgagg cgtcggccag ttggcccagt gagatgggcg cccagtgttc
      301 aaagacatgc gccagcagtt tgcgcggcag ctcggccagt gcatccatgc gcgccttgta
      361 gagcggggtc atggcgtcca gcagcgcttc cagatcactg cgcacgtcgc cgtgtgggct
      421 gtgggcgagc aattcataaa acatcaccgt ggtgcgcggg ttgccgccag acagttggcg
      481 cagggtgggc aggcgctcgg ggtgggcgtt cagttggctt tgcatggcat ggcgcgcgct
      541 gtcgccgcca aaggtagcgg ccagcgccag caaggcttgt tgcatttctt ccagccgcag
      601 cgggcgcaac tcgatattgc ggaagaaatc cagaaacgca tcgtgatagt ggctgtcggc
      661 ttccagactt tggtagctgc cgcccaccca gagcaggcgc gcattgcttt gcagcacctg
      721 gcgcaaactc cagtgggcgg cggcgccgat gttgtgcaac agcaggtcgg tgttgtccac
      781 caagagcagc aggcgttgct gatattggtc ggcgcagtgg ttgatggcgt ccagacaggc
      841 ggcggcttgc tcggcggggg gcaaggcggc caggcgttgg gcctgggcat cgagggcttg
      901 ggtgggttgg ccgcgttgct ccagtgcgtc gatcaggcta tccagcacat tggcccagaa
      961 ttggccaacg gtgtttactg tgtattgctc ttcaggaaag cgcagcgcca gccagtgagt
     1021 gcgcaattcg gcgtcgtcgt ccacggccag cgccagacgt tgcatcaggg tggatttgcc
     1081 catgccgcgc gcgccaatca gcagggtgtg ctgtcccacg ctatcgaccg gggtggttcg
     1141 cagggtgtct aacagctcgc ttaactcgtg ttgtcgggcc acaaagatgg cgcgcagttg
     1201 ctcggcgctc cacaggtgcg gattgtattt gctgatggcg gcaaagccgc cagccggggc
     1261 attgggctga agtgcttcag acatggttgc ggctccacca gtcgcgcagc ggaaacgaca
     1321 aaaattgcac gcgctggctg gcgctgggtg ggctcagata gccttcgtcg ctcagccgca
     1381 ccaacaaatc ttgcaggcgc tgggtgcggc gctcggcgtc ggcctcgtgc ttgctcagcc
     1441 ggctgtgcaa ctggcgcagg gtcaagccgt cggcgtggcg tgaaacgtgt ttgagcaggt
     1501 cctggcacaa tgccagttcg ctggcgggta gtgtggtttt aaggcgtagt tcccagtgat
     1561 aaaagcgcga gcggttggcc agcagttttt cgtaggcatc gtggacatcg ctgagaatta
     1621 gcatgtcgcg gtcggctggc gtggggtttt cttgctggcg ttcgcgcgcc gcgcgcaggg
     1681 tttcgtccag cagcaggcac aaaaaatagg gtgacaacca gcccaggcga gtcagcgcgt
     1741 tttcaatcac ctcatcgctg gcgctgctgc ccgcttgctg ggtgaatttt tgccacatgg
     1801 cccgcgcttc agtcgggcga aaagcaccca gcgggtagtc gtagcagtcg ttcatcttgt
     1861 cggccaggtg gtggcgttcc agcaaggact gcaagcctat cgagccggtg aacacaaagc
     1921 ggcatgggcc gccggtctgt cgccagatgc gcagccaggc cagcaattgc tgcgcatcag
     1981 cggggcgggt gtccagcagg gtttgcagca tcaccggaaa ttcgtcgagc agaatcagca
     2041 aaggttgtgg ctgcaaacgt tggcgcaaga cttcgccagc aaccagccag tctggcatgg
     2101 ggccatcacc caactcgatg cccaggccgc cgccgccgat gatttcgggt aggctggcat
     2161 ctatacgacg caagcggcta aaccagcccg cgatgctttg agcttggtcg ttggcaaatt
     2221 tccgcagggc gggcggtggg taagcttgct ccaacgccag cacaaagctt tcggcgctgt
     2281 ggatgtgcga gacatccaac catcgcgcca gcacaccttg ctcggcggct tgttcttgca
     2341 ggcgtttcag aatggaggtc ttgcccagcc ggcgcacgcc aggaaagcga atgtgctcgt
     2401 tttccaagta gcgccagagg tcgtcaagct cttgtctgcg gccacagaaa tcttctccgg
     2461 tggcgggctg gccaagcatc atggttgggc tcctgaatta attcaaaatt gaatttattc
     2521 gattttgaat taattcacaa ggcccgcccg tcctcaccgc ttgatcagcg gccggttctt
     2581 gtgaatgctc atcaaaatac caaagccgat cagcagggtg accatggcgg tgccgccgta
     2641 agagatcagt ggcagcggca cgccaaccac cggcaggatg ccggccacca tgcccatatt
     2701 gacgaaggcg taggtgaaaa acgacagggt gatcgagccg gccatcaggc ggccaaacag
     2761 tgtggtggct ttggcggcga tcatcaggcc acggccaatc accagcaggt agagcgccag
     2821 caggatgagg ttgccaagca ggccaaattc ttcggcgtac acggcaaaga taaagtcagt
     2881 ggtgcgctcg gggatgaatt ccaggtgggt ttgcgtgccg ctgagccagc ccttgccggt
     2941 ggggccgccg gagccgatgg cgatcatcga ctggatgatg tggtagccgg cgcccagcgg
     3001 gtcggagctg gggtcgatca gggtgagtac gcgcttgcgc tggtaatcgt gcagcatgtt
     3061 ccacacaaac ggcaggctgg cgccaaacgc caccgcgccg gtgatcatca ctttccacgg
     3121 caggccggcg aggaacagca caaaaaaccc cgaggccatg atcagcgtgg cggtgcccag
     3181 gtcgggctgc ttgaggatga acagcgaggg gatgatgatg atcaccgcgc ccaccagata
     3241 atggtgccag cgcagggcgt tttcgtgctt ttggaagtac caggccagca tcatcggcac
     3301 catgattttc agcacttccg agggttggat gcgggtgacg cccacatgca gccagcgccg
     3361 cgagccattg accacttcgc caaacagcgc cacgcccaac agcatcagca cgccgacaat
     3421 atagccgggc acggccagtt gcatcagggt ttgcgggcgg acattggcca cgccccacat
     3481 gatgcccagc gccaccagca tgaaggtcat cttgttgatg actttgtcca gattctggtt
     3541 ggacgccgaa aacaacacca ccaggctgag cgcaaacagc gccatgatca gcgccagcag
     3601 ccagccgtcg atgggcgagc gcagccgctc ccaggcacgg cgcagcaagt aatagtcgtt
     3661 cattccacca cctcttccgc cggggcggcg gcgggtttgt tcttgtcggc gcgcacatgt
     3721 tgcgcgctgg gggcgtcgct attgggggcg ctgacttcgc ctttgcggcc ggtcagataa
     3781 aagtcggcca cctcgcgcgc cagcggtgcg gcggcagcag cgccccaacc gccgttttcc
     3841 acaatcacgg caatggcaat gcgtggcgct tccaccgggg caaacgccac aaaccacgag
     3901 tggtcacggt gttgctcggc cagggcggcg gcgttgtact tggcgccttg cttgatctgc
     3961 accacctgcg cggtgccggt tttgccggcc atgcggtagc tcaggccggc gccaatgcgc
     4021 gcggcggtgc cgccgggttt cagcaccgct tccatggcgg atttcacaaa ggcgatattg
     4081 gccgggtcgg ggtgattgtc gcgcaccgga ttgggttcga ccttggtgat ttcgccggtt
     4141 ttcaggtttt cgatttcctt gaccacgtgc gggcggtaga ccacgccgtt attggccagg
     4201 atggccaccg catgcgccac ttgcagcggg gtgtaggtgt tgtagccctg gccaatgccg
     4261 atgggcacca cgtcggccgg ggtccatacg cgtgcggcct cggggtagcg tttgccggca
     4321 aagcgtttgc gcttccattc cttgctgggc aacaccccgc gtgcctcgcc atccagatca
     4381 atgccggtag ggctgccaaa gccgaatttg gccaactgcg gctccagccg gtcgatgccc
     4441 atgtcccagg ccagcttgta ataataggta tccgacgaca cctgaatcga gcggaacatg
     4501 ctcatgctgc cccagccgct gggcttggaa tcacgaaagc ggtgggtgct gcccggcagc
     4561 gagaaatacc ctggatcagg aatggtgtaa ttggggtcgc ggaatttggt ttccagcgcc
     4621 gccatggcca tgaagggctt gaaggtggag ccaggcggat acaggccgcg cagggcgcgg
     4681 ttgaccagcg gttttttcca gtcgtcgttc aggtctttcc agctctggct gtcgatgccg
     4741 tcgatgaaca gattggggtc gtagcccggt ttggagataa atgccagtac tccgcccgtg
     4801 gccgggtcca gcgccaccag cgcgccacgc cgctcgccaa agcgctccca ggcgaacttt
     4861 tgcagctcga tgtccagcgc cagcttcagc gtgtggccat tgatcggcga cgagcggcgc
     4921 agcgtgcgca ccgggcggcc gccggcgtcg gtttccattt cttcaaagcc ggcggtgccg
     4981 tgcagcactt tttcgtaggt gtattccagc ccggttttgc cgatatgggt gctgccgcga
     5041 taatcggcca gttcgtcgtt ttcttccagc cgctccattt ctttttggtt gatgcggccg
     5101 atgtagccga tcacatgggc ggtgatttcc ttgaagggat aatcgcggaa cagccgtgct
     5161 ttgacctcga cgccgggaaa gcgccagata ttggccgann nnnnnnnnnn nnnnnnnnnn
     5221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5281 nnnnnnnnnn nnnnnnnncc cagcaccacc agggtttgct ggcccaggtt gtacatcacc
     5341 acctggcgct ggcgcgacag cagcagatag ctgatcacgc tataggccag cgcgtgctgg
     5401 ccgaacacgc tggcgctggc cacgtcggca atcaggccga tgccaaacga ccagccaatg
     5461 ccaaaacggc gcggctggtt cagtgtccag tacagggcca gcatggcggt aaagtctggc
     5521 agccagttgg ccagggtggc atccagcggc agcagctcga cggcaatcgc cagcacaaag
     5581 ctcagcacaa tccagcgcgg cttgattggc tttagcagtt ctttgggacg gtcaaacgcc
     5641 atggtttatt catcacttcc acgtttgcgg cgggatttgg tcttgggtgc gggcgccgct
     5701 tccggccggg gtggcaggtt caaggtttcg ttgagcacca gcagcgtgcg gttttcgccc
     5761 acgccggcca gtggggtgca gacaatgcgg gcaaacggcg agccgccgtt gcggtccacc
     5821 atggcgactt tggccaccgg caggccggct gggtagacgc cgtcgatgcc cgaggtcacc
     5881 agcatatcgc ccacctgtac atcggcgtgg gcggcgatat ggcggatttc tgcctggccg
     5941 cccatgccat acatcactgc gcgcagacca ttgcgctgga tcatcaccgg caccggatgg
     6001 ttgcgctcaa tcagcagggt cacttcggcg gtcagcggct gcacgcgggt aatctgcccg
     6061 atcagcccgc gcgcgtcgat caccggcaag ccggcctgca caccgtccat gctgcctttg
     6121 tcgataatca gcgtgaaggt gaacgggtcg cggctggtgt agacgatttc tgccggcagg
     6181 gtgttgccgc catgctggct gcgcaggctc agcgtttgcc gcagctcggc gttttcccgt
     6241 tgcaacgccg cctggcgttg cagacgtgct tccagctcca gctggtaggt gcgcaaagcc
     6301 tggttttcat gcgtcagcgt ggcctgggtg gtcaaaaaag tgtctagctg gcgcaagcct
     6361 tccggtgggg cggtcaccag ccactgcatc gggtagagca gcgcggtcag cgcgccgcgc
     6421 gccgggtcga gcagatgcag cttggcgtcg gccaccagca ggcccagcga caaggcgccg
     6481 cacgccagaa ggcgcgtcag cggcttgggg ccgcgacgaa aaaaacgggg agagctggcg
     6541 atatccatca catgccatca gggggaagtc ggcgcgcgcc agccggacag ggggccggat
     6601 ggcgcgtctt ggtcagcgcg gggtgtcgcg tcagtcgttg gtgaacagcg tgccaacctt
     6661 gtccatcttg tccagcgccc ggcccgagcc gcgcaccacg caggtcagcg gatcgtcggc
     6721 gacgaacacc ggcaggccgg tttcttcttg cagcagacgg tcgatgtcgc gcagcagcgc
     6781 gccgccgcca gtcagcacca tgcctttttc ggcaatgtcg gcgcccagtt ccggcggggt
     6841 ttgctccagg gcgattttca ccgccgagac gatctggttc aaaggctcgg tcagcgcttc
     6901 gaggatttcg ttggacgaca cggtgaaggc gcgcgggatg ccctcggcca gattgcggcc
     6961 tttgacttcc atttcgcgca cttcggcgcc ggggaaggcc gagccgatgg ttttcttgat
     7021 ttcttcggcg gtggtttcac caatcagcat gccgtaattg cgacggatgt aattgatgat
     7081 ggcttcgtcg aacttgtcgc cgcccacgcg caccgagttg gaatacacga tgccgcccag
     7141 cgagatcacg cccacttcgg tggtgccgcc gccgatgtcc accaccatcg agccggtggc
     7201 ttcttccacc ggcaggccgg cgccaattgc ggcggccatc ggttcttcga tcagctcaac
     7261 gcggcgcgcg ccggcgccca gcgccgattc tttgatggcg cgacgctcca cctgggtgga
     7321 gccgcacggc acgcaaatca cgatgcgcgg gctggagctg aacacccggc tggggctgac
     7381 cttcttgatg aactgcttga gcatctgctc ggtgacggtg aagtcggcga tcacgccgtc
     7441 tttcatcggg cggatggctt gaatcgagcc cggggtgcgg ccgagcattt tttttgcttc
     7501 caggccaacg gccaggatgg attttttatt ggatgcgcct tcgtgcatga tcgacaccac
     7561 cgagggttcg tcgagcacga tgcccttgcc ggtcacgtag atcagggtgt tggcagtgcc
     7621 aaggtcgatg gcgaggtcat gagaaaaata accggtaatc gagcgaaaca tcgtagtcct
     7681 gtagtcggcg ggccgcggcg gcgcgtcagg gcgcaggccg gcgcagcgga attttgggtg
     7741 gcaagggtgt cgtgacgttg acacgacggg atgagcctgg ttccgcaagc gtaacgaagc
     7801 cagcatgacg gctggcgggc aattgggcga aaccagcaga ctatgatacc ctacaacatt
     7861 cgacaaatta aagtcttaac gaggttttta tgtccctttc cgcgaccgat gtcagccgga
     7921 tcgcccggct tgcccgcctg cacatggacg agtcggaaat tgccgccgtg cagggccagc
     7981 tcaacgggat ttttggcctg atcgaagcca tgcaggcggt ggacaccact ggcgtggcgc
     8041 ccatgtcgca cccgcaggac gtcagcctgc gcctgcgtga cgacgtggtc agcgaaagcg
     8101 accgccgcgc cgcctaccag gccgtggccc ccagcgtgga aaacggcctt tacctggtgc
     8161 ccaaggtgat tgaataaacc gcgccgctgc ggaccctccc tacagaattc ttttcgtgtg
     8221 cccgtcatga attacacgct caaacagctt tccgacgccc tggccgccgg ccagatgtcc
     8281 agccgcgaac tcacccgcga ctacctcgcc cgcattgacg cccttaaccc gcagctgaac
     8341 gccttcatca ccgtcgatca cgacggcgcc ctggccgccg ccgccgccgc cgacgccgcg
     8401 cgcgccgccg gccaggccgg cgcgctgacc ggcgtgccgt ttgcccacaa ggacgtgttc
     8461 gtcaccgaag gcctgctcac cacctgcggc tcgaaaatgc tgggcaattt tgtcggcccc
     8521 tacgacgctc acgtggtcag ccagtgccgc caggccggcc tggtgatgct gggcaaaacc
     8581 aatatggacg agttcgccat gggctcgtcc aatgaaaatt ccgcctttgg cgcggtgaaa
     8641 aacccgtggg acgtcaaggc cattccgggc ggcagctcgg gcggctcggc cgccgccgtg
     8701 gccgccggcc tggttcccgc cgccaccggc accgacaccg gcggctcgat ccgccagccg
     8761 gccagccatt gcggcatcac cggcatcaag cccacctatg gcacggtcag ccgctatggc
     8821 atggtggcct acgccagctc gctggatcag ggcgggccga tggcgcgcac cgccgaagac
     8881 tgcgcgctgc tgctgaatgt gatggccggc tttgacgcgc gcgactccac ctgcctggac
     8941 cgcgccaaag aagactacac ccgcagcttg aaccatccag tggccggcct gaccatcggc
     9001 ctgcccaaag aattttttgc cgccggcctg tcggccgacg tggcgcgggt gatcgacgct
     9061 gccgtggcgc agttcaaggc gctgggcgcc aagattgtgg aagtcagcct gccgaaaacc
     9121 gagctgtcca tcccggcgta ttatgtgatc gccccggcgg aagcctcgtc caatctgtcg
     9181 cgctacgacg gcgcgcgcta tggccaccgc gccgcgcagt acaaagacct caccgatatg
     9241 tacgaacgct cgcgcgccga aggctttggc gccgaggtca agcgccgcat cctggtgggc
     9301 acgtatgtgc tgtcgcacgg ctattacgac gcgtattaca ttcaggcgca aaaaatccgc
     9361 cggctgatcg ccgaggattt ccaggccgcc ttccagcagt gtgatttcat tctgggcccg
     9421 gtggcgccga cggcggcgtt taatctgggt gagaagtctg acgacccggt gcaaatgtac
     9481 ctgtccgaca tctacacgct ggcggtgaac ctggccggtc tgccgggcat gagcctgccg
     9541 gcggggtttg ccgacaatgg ccgtccggtg ggcgtgcagc tgattggcaa ttacttcagc
     9601 gaagccgcca tgctgggcgc cgcccatcag ttccagctcg ccaccgactg gcacgcccgc
     9661 acgccggcgc tgtgacagcg cgcggccaca caagcacgtt attgcgcaag gaattctagg
     9721 catgaaatgg gaagtcgtga tcgggctgga agtccacacc cagctctcca cccagtcgaa
     9781 aatcttttct ggcagcagca ccgcctttgg cgcggcgccc aacacccagg ccagcgcggt
     9841 ggacatcgcc ctgccgggcg tgctgccggt gatgaaccgc gccgccgtgg acaaggccat
     9901 ccgctttggc ctggcgattg gcgccaagat caaccgcacc tcgatcttcg cccgcaaaaa
     9961 ttatttttac cctgacctgc ccaagggcta tcagatcagc cagttcgagc tgccgattgt
    10021 cgaaggcggc agcctgcaaa ttcaggtggg cgacagcgaa aaaaccgtca acctcacccg
    10081 cgcccacctg gaagaagacg ccggcaagag cctgcacgaa gacttccacg gcatgaccgg
    10141 catcgacctg aaccgcgccg gcaccccgct gctggaaatc gtctccgagc cagaaatgcg
    10201 ctcggccgcc gaagcagtgg cctacgcccg cgcgctgtac accctggtga cctggctggg
    10261 catttgcgac ggcaatatgc aggaaggcag cttccgcgtg gacgccaatg tgtcgattcg
    10321 cccgtttggc caggctgagt tcggcacccg ccgcgagatc aaaaacctga actccttccg
    10381 ctttctggag caggcgatca atgccgaaat ccgctaccag accgagctga tcgaagacgg
    10441 cggcaaggtg cagcaagcca ccgtgctgtt cgacccggac agcggcgaaa cccgcgccat
    10501 gcgcagcaag gaagacgccc acgattaccg ctacttcccc gacccggacc tgctgccgct
    10561 gaaggtcagc gacgccgaag tggacgccat ccgcgccgac atgccggaac tgccgggcga
    10621 gatgaagcag cgctttatcg acagctttgc cgtgtccgcc tacgacgccg gcctgctgac
    10681 gcaatcgctg gcgctggcgc gctactttga agccgccgcc caggcctgcc cgggccaagg
    10741 caagctggtg gccaactggg tgaatggcga agtggccgcg cggctgaacc gcgaagaaaa
    10801 atccatcgcc gactgcccgc tgtccgccga acggctggcc ggcctggtgg cgcgcgtggc
    10861 tgacaacacc ttgtccaata aactggccaa gcaggtgttc gaggcgctgt gggacagcga
    10921 gctgagcgcc gacgcggtga tcgagcgcga cggcctcaag caggtgtcgg acgtcggcgc
    10981 catcgaaaaa ctggtggaag aagccctcgc cgccaacccc aaggcagtgg aagaattccg
    11041 cgccggcaag gaaaaggcgc tgaacgcgct ggccggccag gtgatgaagg ccagcaaggg
    11101 caaggccaac ccggcgcagg tgcaggacat tctgcgccag aagctggctg gctaagcttc
    11161 tgcctgctgg cagtatcgcg ccgcgcccga cctgtgccgg gcgcggcgtt tttgcacaca
    11221 gctttcagtc tgcgactggt tggcgcatct cttgtaccag tgccagccag gcacgcgctg
    11281 cgcgtgacag atagccttcg cgtcgccaga taaaagccag gtcccaggtc atcgccggct
    11341 cgctcagcgc caggcgaacc aagccagggc gtgccagtcc acgcgccacc acgcgtggca
    11401 acagcaccac tccttgaccc gcggccacca gcgtggccag aaaatccgct tggccactgc
    11461 gtccgccttc gcgcggggtg aagcccgcct gccggcaagc ctgccagaga cggtcattca
    11521 gggtaaagct gcgttcgtaa aacaaaaacg gcgtgtccgc cagcgccgcc agcgtgaccg
    11581 cacccgccgt ggcgcatggg tgattggctg gcgccagcac atccagtggc tcatggcaga
    11641 acggcgtatg ggccagctcc gggtgcgatg gcgtcagggc ggcgcccagc tccagcgtgc
    11701 cagtgagcaa ggcttgctca atgcgcttgc tgccaccttc ttccaactgc acgcgtacct
    11761 cggggtagcg gtgcagatat tccacaatca cctgggtgaa cagcgcatcg gcgccaaaca
    11821 agggcaggcc caggcgtagt gctccacgaa tctggggggt ctgatcatcc agttctgcca
    11881 gcattgcctg gccatgtgcc agcatctgct cggcccggcg caaggccact tcgccagcat
    11941 cggtcagcct tagctggccg gggccgcgca ccagtacagg ttggcccagc cgtgcttcca
    12001 gttgggcaat ttgcttactc agtgccgatt gcgtttgatg cagggcgctg gcggcgcggg
    12061 taaagctgcc gtggcggatg atttccacca ggctggccag ttgccgtaat tccggcaggc
    12121 gtgcgtccat ttgtattcct taatggaatt ggcttcatga aaacaattcg tttggggaat
    12181 tttatccgac ctcgtatgct gaccacagtg aaatgtgagt gaggaatgga tgatgtccaa
    12241 cgcgatttct tctgggctgc aagcattggc ccaagtcaca gccttgctgg cgctgtggag
    12301 cttgggcaag gtgctggcgg cctggtgggc gctgccggtg cccggtgggg tgatcgggct
    12361 ggcgttgctg ttgctgctgt ttgcctgccg gctggtgcag ccaggctggt tgcgcgcggg
    12421 tgccggcctg ctgctggccg agatgttgtt gttttttatt ccagcggtga tgtcgctgct
    12481 ggattatggg ccgctgctgc gtgcgcaagg cccgcgtatc ctgctggtga ttgtgtctgg
    12541 caccttgctg gtgatgatga gcactgcgct ggcggtggaa tgggtgtgcc gccgccatgg
    12601 ccacgccctg gaggattgag catgtggcaa atgccgctct ggagttggcc ctggctcacg
    12661 ctgttggcct atgcgctggc gcgcagcctg catcgccgac agcggcgctt ctggctgtca
    12721 ccgattgtgc tggcgccgct gctgctgttt gcgctggcca tccccagcca tacccactat
    12781 gccgattatg cccacgacac cggttggctg gtgagcctgc tgggcccggc caccgtggca
    12841 tttgctgtgc caatctggac gcagcgcgcg ctgctggcgc gtcactggct ggcgctgggc
    12901 gcgggtatgc tggccggttc agccatggct ttgttcagtt cgtggcaact ggcgcatgcg
    12961 ctggcgctgg atggccaggt ggcgcgctca ctgttgccgc gctcaattac cacgccattt
    13021 gccatggaaa tgtcgcgtga tctgggcggt gcgccggagc tgactgccgc atttgtgatg
    13081 gtcaccggca ttgttggcat gctggccggt catttgctgt tgcggctgtt accgctgaaa
    13141 accgcattgg cgcgcggcgc catgctgggt gtggcggccc atggcgccgg ggcggccaag
    13201 gcgcatgagc tgggcgggcc tgagggtacg gtggctggcc tgctgatggt gctgaccggc
    13261 atattcaacc tgctgttggc gccactgctg ctcaagttgt tgtgatgcgg ctggctatct
    13321 aaggccacgc gaccctgagg gtttggtggg cggggctcgc catggcatca tgccctgctg
    13381 cgctgcccgg ccaccccgtg gcggcgcctg aatcctgcct tttatcctac ggacggagcc
    13441 gccatgctaa gccaaaccct gcaagacttt cccctgttga ttctcgatgg cgccttggcc
    13501 accgagctgg agcggcgcgg ctgcgatttg cgcgatgcgc tgtggtcggc gcgcgtgctg
    13561 ctggacaacc ctgagctgat tcgcgccgtg caccacgatt attttgccgc tggcgccgat
    13621 gtggccacca ccgccagtta tcaggccagc tatgaaggct ttgcccggcg cggtttgagc
    13681 gccgagcagg ccaccgcctg cctgcaattg tcggtgcaat tggcgcgtga ggcgcgcgat
    13741 gcgttctggg cggccaatcc gcagctgcac ggcgtgcgcc cgcgccccct ggtggcggcg
    13801 tcggtggggc cgtttggcgc catgcgcgcc gatggctcgg aatacgttgg ccactatggc
    13861 gtggacgacg ccacgctgcg tgattttcat cgcccgcgcc tggcggcgct gctggcggcc
    13921 gggccggatg tgctggcctg cgaaaccctg ccctgcctgc aagaagcgtg cgtgctggct
    13981 gaattgctgc gcgcggagtt tccgcaggcg caggcctggg tcagcttcag ctgccgcgat
    14041 ggcgcgcaca cctgccaggg cgagcccttg gccgaggcgg tggcggcgct ggccgactgc
    14101 ccgcaagtgg tggcggtggg ggtgaactgc accgcgccgg gctttattcc cgagctggtg
    14161 gccgccgccc gcgccgccag cgacaaaccg gtgctggtgt atcccaacgc cggcgaaacc
    14221 tacgacccgg tgcacaaatg ctggcacggc tgcgccgacc cgcaggcctt tgccgaagcg
    14281 gctcgccact ggcatgccca aggcgcgcgg ctgattggcg gctgctgccg caccacgccg
    14341 gaggatattc gcgcgctggc ggcctgggcg cggccggcgg cgacaaatta accttttggc
    14401 attatttttc cggcgtctgg ttccttgacc ggccgtttga tgatggggca atgcaacatg
    14461 catgctgtgg caaaaaaacc accaatgatc cactttgtgc ttgggcacga ttgagcattg
    14521 cgctacgttt gatccgtaga aatacggagg ttttattgtc atggcatcgg aggtgtggca
    14581 ggcaatggcc aatcggcttg ccgcattgca gcatacagaa agggtcgggc tgaggttaaa
    14641 tccatgattt gcatgggcat atttctggag tgctcgctaa gtgcttgtaa aacaagccaa
    14701 aaccccgggg cgtggttgac cggggttttt tttgtcctta tagtggcgcg cagtgggaaa
    14761 aagtggttga aagtgggaaa atcccgataa agcctcaaaa aaaccgcgct tcagcgcgac
    14821 tgccaacaac agcgaacaaa actcggcgca gcccgcctgg ccagatgtat gaccacggtc
    14881 cgtgccagaa gtccggcaag gtcgggtaag tccacctgag caatctgttg gcggcgctca
    14941 gggaaatact ctgctgggca gcgcgggttg acaggacaaa aatggccggc atgtccggcc
    15001 acacccatcc cgccccgtgc gggagcaggg ggctgccaaa cttggcggcc gaatacatcg
    15061 ccacccggcg aaacccttgc ctggcaaggg cgcacggggg cgaatattga aaatacgtga
    15121 accgggtcct tccatgtttg gtggcgtcag cacgctctcg ctcgacagca aaggtcgcct
    15181 ggccattccg gcccggcacc gcgagcgcct gctggccg
//
LOCUS       DAOPYX010000032        15165 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_4242_length_15165_cov_11.248048, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000032 DAOPYX010000000
VERSION     DAOPYX010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 15165)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15165)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15165
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4242_length_15165_cov_11.248048"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..1599)
                     /gene="nifA"
                     /locus_tag="PKN55_02970"
     CDS             complement(1..1599)
                     /gene="nifA"
                     /locus_tag="PKN55_02970"
                     /inference="COORDINATES: protein motif:HMM:TIGR01817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nif-specific transcriptional activator NifA"
                     /protein_id="HNX70238.1"
                     /translation="MMDTPHPPHAAGGQGCHFCPTHSLNLLQLSALYAVSQVLNRTLD
                     TADMLREMLRVLHDEAGLTHGLIAVVEPDSGALMVHSLHHPQAPQPETVRYQPGEGIL
                     GLLLEQPRTVMLKRVADEPRFLNRLGVYAPHLPFIAVPIKVGSAVCGVLAVQPEDEDD
                     GLLSDRGQFVEMVANLIGKSVQLAREVAQERHALIEERDSLRRTVRQQFGFDNVAGHS
                     ASMRRVFDQARQVARWNTTVLIRGETGTGKELIANAIHYHSPRSRHALVKLNCAALPE
                     NLLETELFGHERGAFTGAVDTHKGRFEQAHGGTLFLDEIGEISSAFQAKLLRVLQEGE
                     FERVGGSRTIRVDVRVIAATHRDLEAAVDAGEFREDLFYRLNVMPLFLPPLRERIEDI
                     PDIAQHLLGKIGQAQGRKLRMTDAATSRLAAHHWPGNVRELENCLERAAVLSEDGRID
                     VGLICFPGARERGNARPAARPAPAAHLTPPSVDDPSLSERERVIAALEQAGWVQAKAA
                     RLLGMTPRQIAYRIQTLNIEVKQL"
     gene            complement(1644..3158)
                     /gene="nifL"
                     /locus_tag="PKN55_02975"
     CDS             complement(1644..3158)
                     /gene="nifL"
                     /locus_tag="PKN55_02975"
                     /inference="COORDINATES: protein motif:HMM:TIGR02938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen fixation negative regulator NifL"
                     /protein_id="HNX70239.1"
                     /translation="MSAASAFSPEVSLPPELYRQAVEEADLAISITDRHANILYANAA
                     FTRVTGYQPEQVIGHNESMLSHRTTPREVYQELWSRLAAGQPWNGRLLNRRQDGSVYL
                     AEVSITPVHAPDGSVSHYLGMHRDVTTLRQLECMVNNQKQLIASVLDAAPMVFALLDG
                     NGGLMLSNQAYHSMARELSNDNPAHALMDTLCPDWRAQLASDPAHCTFTDQEARLDRP
                     GGAPRWYTCSAQLIRLSNEAVDGFFCAQNAVGLLLTCTDITALRAEQERARTAALKAI
                     LADEERVASIRESLSAALFRLEEPMNVIFSAVNLLRRRDPASASMLEDALDASRVHLD
                     ALREVIPPRGPETMSNVNLNEILRDVLDIVTPSLLANGVVVDWLPAPTLPTMYGRSVQ
                     LRILFKALIDNAIEAMSGKGWSRRELTLNTRQRGDCIVITVADRGPGMTPDCELRAFE
                     PFFTTKRGGGRHLGTGLSRAYQVATDHGGYIDLGENPGGGCLVTVEFRLDGDPV"
     gene            3628..4206
                     /gene="rsxA"
                     /locus_tag="PKN55_02980"
     CDS             3628..4206
                     /gene="rsxA"
                     /locus_tag="PKN55_02980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012696914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HNX70240.1"
                     /translation="MSEYILLLLSTALVNNVVLVKFLGLCPAMGVSKSVDSALGMGLA
                     TTFVITLAAGASWMLENWLLAPLGLSYLRILTFILVIAAVVQFTEMVIRKMSPGLYQA
                     LGIYLPLITTNCAVLGVALLNISQQFGLFKSLVYGFGSAMGFTLVMVIFAGLRERVAL
                     SRVPQAFDGAPIAFVTISLLALAFMGFSGLNI"
     gene            4226..4756
                     /locus_tag="PKN55_02985"
     CDS             4226..4756
                     /locus_tag="PKN55_02985"
                     /inference="COORDINATES: protein motif:HMM:TIGR01944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HNX70241.1"
                     /translation="MLAAVLSLTVLGAVLGVVLGVANRFLQVEGNPLVDEVLEMMPGS
                     NCGQCGFPGCSGAASALVDGSAAATVCPPGGKALASALAAKLGLSLDLSGMDDSGPKL
                     AAVAEELCIGCCRCAKVCPTDAIVGAARQIHNVLREACTGCESCVPVCPTEALLMKPM
                     PVTLQHWVMPRPAAVA"
     gene            4785..6308
                     /gene="rsxC"
                     /locus_tag="PKN55_02990"
     CDS             4785..6308
                     /gene="rsxC"
                     /locus_tag="PKN55_02990"
                     /inference="COORDINATES: protein motif:HMM:NF003454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HNX70242.1"
                     /translation="MNLRTLLDKLLGSADWGIHPDDHKRPGADAPVRVMPAPSRLYLP
                     LSQHSGAPARPIVLVGQKVKKGECLAEAQGMVSAPVHAPTSGTVLAVADVTAPHPSGL
                     AMPAIMLEPDGLDEWIELKGCDDPFALSPAEINKKVAAAGVVGMGGGTFPSAVKLTGA
                     NRAGIHTVIINGGECEPYLSCDDVLMRTQAAGIVAGVRLIVHASGAKRALIGIEDNKP
                     EAIAAMQAAAAGYDTVRVCPVPARFPMGFDKQMVKTLTGIEVPADGRSFDAGVLVNNV
                     GTALAVWQAVAEGRPLVSRLVTLGGACVNAPGNIDVPLGALAEDVLAFAGGLKGDGLG
                     LARRVIGGPMMGVQVQHWRVPVMKGVSGILALDAKEARLEDASDCIRCGSCVEACPMG
                     LLPSDMAANIRKEALGEAVELGLKDCIACGCCAYVCPSSIPLVQHFTYAKNELAARDR
                     ATLRMEATKKLAQAREARLAREAQEKAEAAARRKAERAAAAAKTAAAAEASKQGEPT"
     gene            6305..7399
                     /locus_tag="PKN55_02995"
     CDS             6305..7399
                     /locus_tag="PKN55_02995"
                     /inference="COORDINATES: protein motif:HMM:NF015100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HNX70243.1"
                     /translation="MMPSSLSGSQLVASPHAHAGKTVTGTMLRVELALVPATLFSFWL
                     YGWPSFLVWALTLASCAGFEALCLRLKGHPQPARVLKDHSALLTGWLLALTLPPWAPW
                     WVPVLGGFIAIVIGKQVFGGLGQNVFNPAMVARVALLVSFPLPLTTWVLPGAQASLPA
                     PGLADSLMIFLGAMPIPDAMASASPLGFAKTELSRGVDLLHSLSSGHAPLAAWHGVRV
                     GSLGESAAPLLLAGGLYLIAVRIISWHIPLAVLAGLAIPAAIGHAANPAHYLDAATHL
                     LSGAAILGAFFIATDYVTSPTTPLGKLLFGLGIGFLTWIIRTWGGYPEGMAFAVLFMN
                     ALAPMLDRAFKPRILGRQRNGKPLDAPKEA"
     gene            7401..8009
                     /gene="rsxG"
                     /locus_tag="PKN55_03000"
     CDS             7401..8009
                     /gene="rsxG"
                     /locus_tag="PKN55_03000"
                     /inference="COORDINATES: protein motif:HMM:NF002519.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HNX70244.1"
                     /translation="MNLDAIREKLGYQPVLLGVVALVVSGALALAATSTHDAIAAAEA
                     ADLRNSLSDVLPKGFADNDLVKDVVTLQYKDKPLTIYRARLGSALKGVVFQVSERGYS
                     GEILVLMAVDVDGKMLGVRVLKHTETPGLGDKIEIAKDKWILSFNGKSLGEPPADKWG
                     VKKDGGVFDQFAGATITPRAVVKAVKGGMLFFSEHKAEILGG"
     gene            8012..8707
                     /locus_tag="PKN55_03005"
     CDS             8012..8707
                     /locus_tag="PKN55_03005"
                     /inference="COORDINATES: protein motif:HMM:NF009070.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit E"
                     /protein_id="HNX70245.1"
                     /translation="MADSKAPALGNVMKEGLWGQNVVFSQLLAMCPTMAVTTSGTNGL
                     GMGLATTAVLIVSNILVSMIRHTVSSQVRIPVFVVVIAGLVTVVDMVLNAWMHDLYKV
                     LGLFIALIVVNCAILGRAEAFAVKNGVLISAVDGLAMGLGFTGALTFVGLLREFFGAG
                     TLFSNASNLLGPSFAFLEMKLPGYGGALLMILPPGGFAVLGFILAGKRAMELRQARLA
                     KAAPPPAAQPSHC"
     gene            8846..9118
                     /locus_tag="PKN55_03010"
     CDS             8846..9118
                     /locus_tag="PKN55_03010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018748296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HNX70246.1"
                     /translation="MQVGVVYSEPGQQVWLNLEVPDESTVVEAIERSGILKQFPHVAL
                     AERKLGIFGRVVKPDAVLKAGDRVEIYRPIIADPETVPRRDTGGEE"
     gene            9409..10737
                     /locus_tag="PKN55_03015"
     CDS             9409..10737
                     /locus_tag="PKN55_03015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX70247.1"
                     /translation="MTIPSRIALLGAGLALPMSALAGNGVSVGGIPLEFFLFAATLLG
                     VALFHHFSLWVALGGFAVISVYKIAVTGFVTGAGLAGFAGHLHHEWVTVANLFCLLTG
                     FAILARHFEKSHVPVILPKYLPDDWKGGFALLVMVFVISSFLDNIAAALIGGAMAHQL
                     FKAKVHIGFLAAIVAASNAGGSGSVVGDTTTTMMWIDGVSPLIVLDAYVAAVVALVIV
                     GYFGAKQQHAYSPILKNSHAHTHVDWGRLLIVGLMLVFAVGANVTVNTFYTSMADSFP
                     FIGVAVWSAILLTIPVRRHDWEVLPETVQGSIFLLSLVMCASMMPVEQLPPASWMSAL
                     ALGFISAIFDNIPLTALALKQGGYDWGFLAYAVGFGGSMLWFGSSSGVALTNMFPEGR
                     SAGQWLRHGWHVPVGYVAGFAVLLFTLGWHPDAPHGEPKTPPAAVSAPAS"
     gene            11350..>15165
                     /locus_tag="PKN55_03020"
     CDS             11350..>15165
                     /locus_tag="PKN55_03020"
                     /inference="COORDINATES: protein motif:HMM:TIGR03903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOMM system kinase/cyclase fusion protein"
                     /protein_id="HNX70248.1"
                     /translation="MRIEIPGYTLESLLGAGSYGAVYLARADKNGQTVAIKIAHVHDD
                     ARRIERFRRETSLCSRLRHPSIVQLLDDGVCGQYVYGVFEYVPGVTLKQRLLQEGPLD
                     PLETGRLMSQVLDALACAHQLGIVHRDLKPENIMIARYGVVNHAKVLDFGISTVVPDL
                     REPHFKNMTLADECLGTPTYCAPEQLRGENPSVQADLYAWALVFIECLTGRPVFEGVS
                     AADIFYQKLVPQETVLPKSIGLHPVATLLRKALRKKRVERHASAGDMLLELSRIRLDD
                     LVGVSLPQAAAERAAELPTTICQPSREEKRQVTILCCSVSVCQPDAGDGLDSDGFEQR
                     ESLQRTLCQAFQEAALMRGGTLIGSLGHQFSVMFGYPRSTEGDARLAASMAVELALLA
                     RERQGDAALRRAGAELEVRIGLHAGMVVAADGEVSPGNTLNIALQMESHAGPGEIWAS
                     EAYRQLLRGLAEFEPAGRVRVPSASATVGCFKLLAHTLQPIEVAAGSDSWHTRCIGRA
                     RELRQVLRGWRQARQGQGGGWLLRGEAGVGKSCLSETLRRHIASRGGLTLCARCFPEY
                     ENNALSPLLSLLRQHVLAGVEPVAQIARLTQVLHDAGCDVDRVMPIFCAWLALPFARQ
                     DAGAVSPVLQKQLLIEALGQWLSHLARQTPVLLVVEDLHWADSATLDALHHMAGMLAA
                     QPLALLMTCRPGAVLQADSVQVLELARLKPNEAAAMVRHQFAPRLLADSVVDSIVART
                     EGVPLFIQELSHVLRDTSLVETDGVWSFKPGAQVRAIPITLRESLHSRFDQLGPARDI
                     LQLAATIGREVDGGLLRVCAAEAGPDALALALDILVDAGLLARADDDRYVFRHALIRD
                     AAYDIMLISQQRRRHEVVAQAIVEHDPQQLSDAPGSVAHHFARAEHYPRAVELGLAQL
                     RLTQHRSLNDETIAYADQVNEWINRLEGRAQLEARLELNGCVTQALMNKYGWAHPQVA
                     EKIALSESILAQDISAELRLQHLWTTLTFHHVASNRADVMQLAQAMLQHGQARDDSHA
                     LVSARLWLGLAQYSEGRFDDAERELSAAIDAHDHEAHAGHAVRFGLDTLVWSRAARAL
                     VRWGLGYEQSARDDARVAVARARQLAHAPSLGIALLYLGLFELGRDDATAAQAVCDEL
                     IELAHRYGLPAFLGYAQIIRSWAVGDTAHADAGIQALWSIGCRYCQTYYRAFAGHTLA
                     RAGDLAGALARLDDSLSLVEPLAERLHESELYWHKARYLRASGAEASQVRAAAAADSA
                     RAGGKCRTEA"
BASE COUNT         2389 a         4785 c         5045 g         2946 t
ORIGIN      
        1 ttacagctgc ttgacctcga tattcaaggt ctggatgcga taggcaatct gccggggggt
       61 catgcccaac aggcgcgcgg ctttggcctg cacccagccg gcttgctcca gcgcggcgat
      121 cacccgctcg cgctcggaca gcgaggggtc gtccaccgag ggcggggtca ggtgggcggc
      181 gggggcgggg cgagccgccg ggcgcgcgtt gccgcgttcg cgcgcgcccg gaaaacagat
      241 caagcccacg tcgatgcggc cgtcttctga caacaccgcc gcccgctcca gacagttttc
      301 cagctcgcgc acattgcccg gccagtggtg ggcggccagc cggctggtgg cggcgtcggt
      361 catgcgcagc ttgcggccct gcgcctggcc gattttgccc aacagatgct gggcaatgtc
      421 cgggatgtcc tcgatgcgct cgcgcagcgg cggcagaaac aagggcatca cgttcagccg
      481 gtaaaacaaa tcctcgcgga attcgccagc gtccaccgcc gcttccaggt cacggtgggt
      541 ggcggcgatc acccgcacgt ccacgcggat ggtgcggctg ccgcccaccc gctcgaactc
      601 gccttcctgc aacacccgca acagcttggc ctgaaacgcc gaagaaattt cgccgatttc
      661 gtccagaaac aaggtgccgc catgcgcctg ctcaaaacgg cccttgtggg tgtccaccgc
      721 gccggtgaag gcgccgcgtt cgtggccaaa cagctcggtt tccagcaggt tttccggcag
      781 cgccgcgcag ttgagcttga ccagcgcatg gcgcgagcgc ggcgagtggt aatgaatggc
      841 gttggcgatc agctccttgc cggtgccggt ttcgccgcga atcagcaccg tggtgttcca
      901 gcgcgccacc tggcgcgcct ggtcgaacac ccggcgcatg ctggccgagt ggccggcgac
      961 attgtcaaag ccaaactgct ggcgcacggt gcggcgcagc gagtcgcgct cttcgatcag
     1021 cgcgtggcgc tcctgcgcca cttcgcgcgc cagctgcacg ctcttgccga tcaggttggc
     1081 gaccatttcc acaaactggc cacggtcgct gagcaagccg tcgtcttcgt cttccggctg
     1141 caccgccagc acgccgcaca ccgcgctgcc aaccttgatt ggcacggcga taaacggcag
     1201 gtgcggggcg tacacgccca gccggttgag aaaacgcggt tcatcggcca cgcgcttgag
     1261 catcaccgtg cgcggctgct ccagcagcag gccgagaatg ccctcgccag gctgatagcg
     1321 caccgtttcg ggttgtggcg cttgtggatg gtgcaggcta tgcaccataa gtgcgccgct
     1381 gtccggctcg accacggcaa tcaggccgtg ggtgaggccg gcttcgtcgt gcagcacgcg
     1441 cagcatttcg cgcagcatgt cggcggtgtc cagggtgcgg ttgagcacct ggctgaccgc
     1501 gtacagcgcc gacagctgca acaggttcag gctatgggtg gggcaaaaat gacaaccttg
     1561 tccaccagcc gcatgcgggg ggtgcggggt atccatcatt cagttcgctc tatccggact
     1621 caggggcatt gacgacgcca ggctcatacc gggtcgccgt caaggcggaa ttccacggtc
     1681 accaggcagc cgccgccagg gttttctccc aggtcgatat agccgccgtg gtcggtcgcc
     1741 acctggtagg cgcgcgacaa gccggtgccc aggtggcggc cgccgccgcg tttggtggtg
     1801 aaaaacggct caaaggcgcg cagctcgcaa tccggggtca tgcctggccc ccggtcggcg
     1861 acggtgatga caatacagtc gccgcgctgg cgggtgttca gcgtcagctc gcgccgtgac
     1921 cagcccttgc cgctcattgc ctcgatggcg ttgtcgatca gggccttgaa cagaatgcgc
     1981 aactgcaccg agcggccata catggtgggc agggtgggcg ccggcagcca gtccaccacc
     2041 acgccattgg ccagcaggct gggggtgacg atgtccagca cgtcgcgcag gatttcgttg
     2101 aggttgacgt tgctcatggt ttccggcccg cgcggcggaa tcacctcgcg cagcgcgtcc
     2161 agatgcacgc ggctggcgtc cagcgcgtct tccagcatgc tggcgctggc cgggtcgcgc
     2221 cggcgcagca ggttcaccgc cgagaaaatc acattcatcg gctcttccag ccggaacagg
     2281 gccgccgaca ggctttcgcg aatcgacgcc acccgttctt cgtcagccag aatggccttc
     2341 agcgcggcag tgcgcgcgcg ctcctgctcg gcgcgcagcg cggtgatgtc ggtgcaggtc
     2401 agcagcaggc caacggcgtt ttgcgcgcag aaaaacccat ccaccgcctc attgctcaga
     2461 cgaatcagct gggcgctgca ggtgtaccag cgcggcgcgc cgccaggccg gtccagccgg
     2521 gcttcctggt cggtgaaggt gcagtgcgcc gggtcgctgg ccagttgggc gcgccagtcc
     2581 gggcacagcg tgtccatcag cgcgtgcgcc ggattgtcgt tgctcagctc gcgcgccatg
     2641 ctgtggtagg cctgattgct cagcatcagc ccgccattgc catccagcaa ggcaaacacc
     2701 atcggcgcgg cgtccagcac cgaggcgatc agttgttttt ggttgttcac catgcattcc
     2761 agctggcgca gggtggtgac gtcgcggtgc atgcccagat agtggctgac gctgccatcc
     2821 ggcgcgtgca ccggggtgat gctgacctcg gccaggtaca cgctgccatc ctgccggcgg
     2881 ttgagcagcc ggccattcca gggttggccg gcggccagcc ggctccaaag ctcctggtac
     2941 acctcgcgcg gggtggtgcg gtgcgacagc atggattcgt tgtggccaat cacctgctcg
     3001 ggctggtagc cggtgacgcg ggtgaaggcg gcgttggcgt acaggatatt ggcgtggcgg
     3061 tcggtgatgg agatggccag atcggcttct tccaccgcct gtcggtacag ctccggcggt
     3121 aatgaaacct cgggggagaa agcggatgcg gccgacatga tgggtctctc cttgcgtgcg
     3181 gatgtctgtc cggtttggcc ccgcgcgcat gcggcgggct gtggggcgtg tgggcgtcag
     3241 gccgcaggag ggtgtaaagc gtcgctcaca aaaccctcct ggcgttgttg tgcggccttg
     3301 ccgtactgct tgtgctgtct gcggccgcac gcctggccag gatcgcttcg ctgagctttg
     3361 tgagcgacgc taaataaacc tgcgggctgg cgcgcaataa ggcttagcag gaatcgtgcc
     3421 gaccatcgaa atcgggtaaa tcgctgaaat cgctcgccaa gggacaaaac tgacgaaacc
     3481 cctgtcggat acccgacatt gcgttgcaac aatgtttgcc gtcaaaacaa aaaatccagt
     3541 ttatacatag gtttatgttt tctcaggccg gcgtggcatg ccttatgcac aggcgcggac
     3601 attcccaaat ccgtctgtga gcgcatcatg agcgaatata ttctcctgtt attgtccact
     3661 gccctggtca ataacgtggt gctggtgaag tttctcggct tatgcccggc catgggggtg
     3721 tccaagagcg tggacagcgc gctgggcatg ggcctggcca ccacctttgt catcaccctg
     3781 gcggctggcg cgtcctggat gctggagaac tggctgctgg cgccgctggg gctttcttac
     3841 ctgcgcatcc tcaccttcat tctggtgatt gccgccgtgg tgcagttcac cgaaatggtg
     3901 attcgcaaga tgagccccgg cctgtaccaa gcgctgggca tctatctgcc gctgatcacc
     3961 accaactgcg ccgtgctggg cgtggcgctg ctcaatattt ctcaacaatt tggcctgttc
     4021 aagagcctgg tgtacggctt tggctcggcc atgggcttta ccttggtgat ggtgatcttt
     4081 gccggcctgc gcgaacgcgt ggcgctgtcg cgcgtgccgc aggcctttga cggcgcgccc
     4141 atcgcctttg tcaccatcag tttgctggcg ctggccttta tgggcttctc cggcttgaac
     4201 atctgaatct ctgaaaggaa ccgtcatgct tgccgctgtc ttgagtctga ccgtgctggg
     4261 cgccgtgctg ggcgtggtgc tgggggtggc caaccgcttt ttgcaggtgg aaggcaatcc
     4321 gctggtggat gaggtgctgg agatgatgcc tggctccaac tgtggccagt gcggttttcc
     4381 gggctgttct ggcgccgcct cggcgctggt ggatggctcg gccgccgcca ccgtctgccc
     4441 gcccggcggc aaggcgctgg cctcggcgct ggccgccaag ctggggctga gcctggattt
     4501 gtccggcatg gacgacagcg gccccaagct ggccgccgtc gccgaagaac tctgcatcgg
     4561 ctgctgccgc tgcgccaagg tgtgccccac cgacgccatc gtcggcgccg cccgccagat
     4621 tcacaacgtg ctgcgcgaag cctgcaccgg ttgcgaaagc tgcgtgccgg tgtgccccac
     4681 cgaagccctg ctgatgaagc cgatgccggt caccctgcaa cactgggtga tgccgcgccc
     4741 ggccgccgtg gcctgaatcg ctcaatcgtc aaggaaatcc tgtcatgaac ctgcgcacct
     4801 tgctggataa attactgggc agcgccgatt ggggcattca ccccgacgac cacaagcgcc
     4861 ctggcgccga tgcgccggtg cgggtgatgc ccgcgccatc gcggctgtat ctgccgctgt
     4921 cgcaacactc gggcgccccg gcccggccga ttgtgctggt gggccaaaaa gtcaaaaaag
     4981 gcgaatgtct ggccgaagcc cagggcatgg tgtccgcgcc ggtgcacgcg ccgacctccg
     5041 gcacggtgct ggcggtggcc gatgtgaccg cgccgcaccc ctccggcctg gccatgcccg
     5101 ccatcatgct tgagccggat ggcctggatg aatggattga gctcaaaggc tgtgacgacc
     5161 cctttgccct gtcgccggcc gagatcaaca aaaaggtcgc cgccgctggc gtggtgggca
     5221 tgggcggcgg cacctttccg tcggcggtca agctgaccgg cgccaaccgc gccggcattc
     5281 acaccgtcat catcaatggc ggcgagtgcg agccctatct gtcctgcgac gacgtgctga
     5341 tgcgcaccca ggcggccggc atcgtcgccg gcgtgcggct gatcgtgcac gccagcggcg
     5401 ccaagcgcgc gctgatcggc attgaagaca ataaacccga agccatcgcc gccatgcagg
     5461 ccgccgccgc cggctacgac accgtgcgcg tgtgcccggt gccggcgcgt tttcccatgg
     5521 gctttgacaa acagatggtc aagacgctca ccggcatcga agtgccggcc gatggccgct
     5581 cgtttgacgc tggcgtgctg gtcaacaatg tgggcacggc gctggccgtg tggcaggcgg
     5641 tggccgaagg ccggccgctg gtgtcgcggc tggtgacctt gggcggcgcg tgcgtgaacg
     5701 cgccgggcaa tatcgacgtg ccgctgggcg ccttggctga agacgtgctg gcctttgccg
     5761 gcggcctcaa gggcgacggc cttggcctgg cgcgccgggt gattggcggg ccgatgatgg
     5821 gcgtgcaagt gcagcattgg cgcgtgccgg tgatgaaggg cgtcagcggc attctggcgc
     5881 tggacgccaa agaagcccgg ctggaagacg ccagcgactg catccgctgc ggcagctgcg
     5941 tagaggcctg cccgatgggc ctgctgccct cggacatggc cgccaatatc cgcaaggaag
     6001 ccctgggcga agccgtggag ctgggcctga aagactgcat tgcctgcggc tgttgcgcct
     6061 atgtgtgccc gtccagcatt ccgctggtgc agcacttcac ctacgccaaa aacgaactgg
     6121 ccgcccgcga ccgcgccacc ctgcgcatgg aagccaccaa gaaactcgcc caggcgcgcg
     6181 aagcgcggct ggcgcgcgaa gcccaggaaa aggccgaagc cgccgcccgc cgcaaggccg
     6241 agcgcgccgc cgccgccgcc aaaaccgccg ccgcggctga ggcaagcaag caaggagaac
     6301 cgacatgatg ccgtcgtctt tgtcgggcag ccagctggtt gcctcgccgc acgcccatgc
     6361 gggcaaaacc gtcacaggca ccatgctgcg agtggagctg gcgctggttc cggccacctt
     6421 gttttcgttt tggctgtatg gctggccgtc gtttctggtg tgggcgctga ccctggcctc
     6481 ttgtgccggg tttgaggcgc tgtgcctgcg gctgaagggc catccgcagc cggcgcgcgt
     6541 gctcaaggac cattccgccc tgctcacggg ttggctgctg gcgctgacct tgccgccgtg
     6601 ggcgccgtgg tgggtcccgg tgctgggcgg ttttatcgcc atcgtgattg gcaagcaggt
     6661 gtttggcggc ctggggcaga acgtgttcaa cccggccatg gtggcgcggg tggcgctgct
     6721 ggtgtcgttt ccgctgccgc tgaccacctg ggtgctgccg ggcgcgcaag ccagcctgcc
     6781 ggcgccgggc ctggccgaca gcctgatgat ttttttgggc gccatgccga ttccggacgc
     6841 catggccagc gcctcgccgc tgggctttgc caaaaccgag ctgtcgcgcg gcgtggattt
     6901 gctgcacagc ctgagcagcg gccatgcgcc cttggccgcc tggcacggcg tgcgggtggg
     6961 cagcctgggt gaaagcgccg cgccgctgct gttggccggc gggctgtacc tgatcgccgt
     7021 gcgcattatc tcctggcaca tcccgctggc ggtgctggct ggcctggcca tcccggccgc
     7081 catcggccac gccgccaatc cggcgcacta cctggacgcc gccacccacc tgctgtctgg
     7141 cgcggcgatt ctgggcgcgt ttttcatcgc caccgactat gtgacctcgc cgaccacgcc
     7201 gctgggcaag ctgctgtttg gtctgggcat tggctttttg acctggatca tccgcacctg
     7261 gggcggctac ccggaaggca tggcgtttgc cgtgctgttc atgaatgcgc tggcgccgat
     7321 gctggaccgc gccttcaagc cgcgcatcct cggccgtcaa cgcaacggca aaccgctgga
     7381 cgcccccaag gaggcctgac atgaatctgg atgcaattcg ggaaaaactg ggctatcaac
     7441 ccgtgctgtt gggcgtggtg gccctggtgg tcagcggcgc gctggcgctg gccgccacca
     7501 gcacccacga cgccatcgcc gccgccgagg ccgccgacct gcgcaattcg ctcagcgacg
     7561 tgctgcccaa gggctttgcc gacaacgacc tggtcaagga cgtggtcacc ctgcaataca
     7621 aggacaaacc gctgaccatc taccgcgccc ggctgggcag cgcgctcaag ggcgtggtgt
     7681 ttcaggtcag cgaacgcggc tacagcggcg agattctggt gctgatggcg gtggatgtgg
     7741 acggcaagat gctgggcgtg cgcgtgctca agcacaccga aacccctggt ctgggcgaca
     7801 agattgaaat cgccaaagac aaatggattc tcagcttcaa cggcaaatcg ctgggcgagc
     7861 cgccggcaga caaatggggc gtgaaaaaag acggcggcgt gtttgaccag tttgccggcg
     7921 ccaccatcac cccgcgcgcc gtggtcaagg cggtaaaggg cggcatgctg tttttcagtg
     7981 aacacaaagc ggaaattctc ggaggctgat catggctgac agcaaggcgc ccgcgctggg
     8041 caatgtaatg aaagaaggcc tgtggggaca aaacgtggtg ttctcgcaac tgctggccat
     8101 gtgtccgacc atggcggtga ccaccagcgg caccaatggc ctgggcatgg gcctggccac
     8161 cacggcggtg ctgattgtgt cgaatattct ggtgtcgatg attcgccaca ccgtcagctc
     8221 gcaagtgcgc atcccggtgt ttgtggtggt gattgccggc ctggtgaccg tggtggacat
     8281 ggtgctcaac gcctggatgc acgacctata caaggtgctg ggcctgttta ttgcgctgat
     8341 tgtggtcaac tgcgccatcc tgggccgcgc cgaggcgttt gccgtgaaaa acggcgtgct
     8401 gatttccgcc gtggacggcc tggccatggg cctgggcttt accggcgcgc tgacttttgt
     8461 cgggctgctg cgtgagttct ttggcgccgg cacgctgttc agcaatgctt ccaatttgct
     8521 tgggccgagc tttgccttcc tggaaatgaa actgcccggc tacggcggcg ccctgctgat
     8581 gattctgccg ccgggtggct ttgccgtgct gggctttatc ctggcgggca agcgcgccat
     8641 ggagctgcgc caggcccgtc tggccaaggc cgccccgccg cccgccgccc agccttcgca
     8701 ctgctaagag cggctaacaa aaccctcctg gcgttgttgc ccgactgttg tactgtctgc
     8761 ggtcgtgcgc ctagccagga tcgcttcgct gggttttgtg agccgctcta agccgacatc
     8821 gacgacatca tcaaggagtt tcatcatgca agtgggtgtg gtctattccg aaccgggcca
     8881 gcaagtctgg ctgaatctgg aagtgccgga cgaatccacc gtggtcgagg ccatcgagcg
     8941 ctctggcatt ctcaagcagt ttccgcatgt ggcgctggcc gagcgcaagc tgggcatctt
     9001 tggccgcgtg gtcaagcccg acgccgtgct caaggccggc gaccgggtgg aaatctaccg
     9061 gcccatcatc gccgaccctg aaaccgtgcc gcgccgcgac acgggcgggg aggaatgatc
     9121 gggataaccc gaatccatgg ccgaaagtgt cttgggtata ctgccggcct tggcaagttg
     9181 ttctggccgc ctgtgctggg cgccgctcac aaaaccctcc tggcgttgtg acgctgcgct
     9241 gagtgtggtg agcggcgctg aagaagcgcc cacccaatct ttactggcgc ggttgggtgg
     9301 cgcccacatt ctacgcaaca ggcggcgcct cctggcccgc gcgcctgccg cagcgcgtcg
     9361 cgtcttgacg gggcgattgc cgttttttca acctttgagt aagccatcat gactattccc
     9421 tcgcgcatcg ccctattggg cgcgggcctc gcgctgccca tgtcggcgtt ggccggcaat
     9481 ggcgtcagcg taggcggcat cccgctggag ttctttttgt ttgccgccac cctgctgggc
     9541 gtggcgctgt ttcaccactt ctcgctgtgg gtggcgctgg gcggttttgc cgtcatcagc
     9601 gtgtataaaa tcgccgtcac tggctttgtg actggcgcgg gtctggccgg ttttgccggg
     9661 catttgcacc acgaatgggt gacggtggcc aatttgttct gcctgctcac cggctttgcc
     9721 atcctggccc ggcattttga aaaaagccat gtgccggtga ttctgcccaa atacctgccc
     9781 gacgactgga agggcggctt tgcgctgctg gtgatggtgt ttgtgatttc cagctttctg
     9841 gacaatatcg ccgccgcgct gattggcggc gccatggccc accagctgtt caaggccaag
     9901 gtgcatatcg gttttctggc ggcgattgtc gccgcgtcca atgccggcgg ttccggctcg
     9961 gtggtgggcg acaccaccac caccatgatg tggatcgacg gcgtcagccc gctgattgtg
    10021 ctggatgcct atgtggccgc cgtggtggcg ctggtgattg tcggctactt tggcgccaag
    10081 cagcagcacg cctactcgcc catcctcaag aacagccacg cccacaccca tgtggattgg
    10141 gggcgcttgt tgattgttgg cctgatgctg gtgtttgccg tgggcgccaa tgtcaccgtc
    10201 aacacgttct ataccagtat ggccgacagc tttccgttta tcggcgtggc ggtgtggagc
    10261 gccatcctgc tgactatccc ggtgcgccgt cacgactggg aagtgctgcc ggaaaccgtg
    10321 caaggttcga tcttcttgct gtcgctggtg atgtgcgcgt cgatgatgcc ggtggagcaa
    10381 ctgccgccgg cgtcgtggat gagcgccttg gcgctgggct ttatctcggc gatttttgac
    10441 aacatcccgc tcaccgcgct ggcgctcaag caaggcggct acgactgggg ctttttggcc
    10501 tacgccgtcg gctttggcgg ctcgatgctg tggtttggtt cgtcctctgg cgtcgcgctg
    10561 accaatatgt tccccgaggg ccgctccgcc gggcaatggc tgcgccacgg ctggcatgtg
    10621 ccggtgggct atgtggccgg ctttgccgtg ctgctgttca ccctgggctg gcatccggat
    10681 gcgccgcatg gcgagccgaa aaccccgccg gcagcagtga gcgccccggc ttcctgagct
    10741 tgcccgcttg ataccctggc cgcctttggg gacggccgac gctgcgtctg attgatcacg
    10801 ccgcgcctct tcctggggcg cggcgtgatg ttttctgcct tgctgttcct gacatgatgc
    10861 gtgattacaa cacttgcgcc gtgctttctc cctgctgccg gctatcctga tgccatggtt
    10921 gttatgaggt gtaattgagg cagctatccg ggatatgggt caatatgcgt agatttactc
    10981 aagtagtttt acgtatttag caaaatggca ttgatttttg ggcggtgccg acttctgggc
    11041 gcccagtctg tttgccaggt tggccaagca tccgctgcgg cggaaggtgt gatgtcatgt
    11101 gtcgggaatg cgctgccctg tgtcactatg gggccactcg acctgatgcc gccgtggctg
    11161 tgtatccata tggcgttttg agtcggagag ttcagcatga tgcaggtgcg caggtttcat
    11221 gtgttggcca tgacgtccat ggatttggca tggggcatgc gcgcctgagc caaccaagga
    11281 caaagaccag ccatgaaagc gcctcttgcc ggggacatcc cccgcccgct ccccccttcc
    11341 gggtcacccg tgcgcattga gatccccggt tacaccctgg agtccttgct gggcgccggc
    11401 agttatggcg ccgtctatct ggcgcgtgcc gacaagaatg gccagacggt agccatcaaa
    11461 attgcccatg tgcacgacga cgcccgccgc attgagcggt ttcgtcgcga aacctcgctg
    11521 tgctcacgct tgcgccatcc cagcattgtg caactgctgg atgacggtgt atgcgggcag
    11581 tatgtgtatg gggtgtttga atatgtcccc ggcgtcaccc tcaagcagcg cctgctacag
    11641 gaaggcccgc tggacccgct ggaaaccggc cggctgatga gccaggtgct ggatgcgctg
    11701 gcttgcgccc accaactggg cattgttcac cgcgacctca agccagaaaa catcatgatt
    11761 gcccgttatg gggtggtcaa ccatgccaag gtgctggatt ttggtatcag caccgtggtg
    11821 cccgacctgc gtgagccgca cttcaagaac atgaccctgg cagacgaatg cttgggcacg
    11881 cccacctact gcgcgccgga acaattgcgc ggcgaaaacc ccagtgtgca ggccgatttg
    11941 tacgcctggg cactggtgtt catcgagtgc ctgactggcc gtccggtatt tgaaggcgtg
    12001 tcggcggcgg atatttttta tcaaaagctg gtgccgcaag aaaccgttct gcccaagtct
    12061 attggtctgc acccggtggc tactttgttg cgcaaggcct tgcgcaagaa gcgcgtcgag
    12121 cggcatgcct cggcgggcga catgctgctg gagctgtcgc gcatccgcct ggacgatctg
    12181 gtgggcgtat cgctgccgca agcggccgca gagcgcgccg ccgagctgcc caccaccatc
    12241 tgccagccca gtcgtgaaga aaagcgtcag gtcacgatct tgtgttgcag cgtttcggtc
    12301 tgtcagccgg atgccggtga tggcctggac agcgatggtt tcgaacagcg tgaaagtctg
    12361 caacgcacct tgtgtcaggc cttccaggaa gccgcgctga tgcgcggtgg cacactgatc
    12421 ggctcgctgg gccaccagtt cagtgtgatg tttggttacc cacgcagcac cgaaggtgat
    12481 gcccggctgg ccgccagcat ggccgtggag ctggcgttgc tggcgcgcga gcgccagggc
    12541 gatgctgcct tgcgccgtgc cggcgccgaa ttggaagtgc gcattggcct gcacgccggc
    12601 atggtggtgg ccgctgacgg cgaagtctcg ccaggcaata ccctcaatat tgccctgcaa
    12661 atggaaagcc acgccggccc cggcgaaatc tgggccagtg aagcgtatcg ccaactgctg
    12721 cgtggcctgg ccgaattcga accggccggc cgggtgcgtg tgccgtcggc gtcggccacg
    12781 gtgggctgct tcaaactctt ggcccacacc ttgcagccca tcgaagtggc ggccgggtcg
    12841 gattcctggc acacccgctg catcggccgc gcccgtgagc tgcgccaggt gttgcgcggt
    12901 tggcggcagg cccgccaggg ccagggcggc ggctggttgc tacgcggcga ggccggcgtg
    12961 ggtaaatcct gcctgagcga aaccctgcgt cggcatatcg ccagccgtgg cggcctgacg
    13021 ctgtgcgcgc gttgtttccc ggaatacgaa aacaacgcgc tgtcgccgct attgtccttg
    13081 ctgcgccagc atgtgctggc cggcgtcgag ccggtggcgc agattgcccg cctcacccag
    13141 gtattgcatg acgccggctg cgatgtggat cgggtgatgc cgattttttg cgcctggctg
    13201 gccttgccgt ttgcccgcca ggatgccggt gcggtgtcgc ccgtgctgca aaagcagttg
    13261 ctgatcgagg cattgggcca gtggctgagt catcttgccc ggcaaacccc ggtgctgctg
    13321 gtggtggaag acctgcactg ggcggattcg gccacgctgg atgccttgca ccatatggcc
    13381 ggcatgctgg ccgcgcaacc gctggcgctg ctgatgacct gccgtccggg tgccgtattg
    13441 caggcggatt cggtgcaggt gctcgaactg gcgcggctca agccaaacga agccgccgcc
    13501 atggtgcgcc accagtttgc cccgcgcctg ctggccgaca gtgtggtgga cagcattgtt
    13561 gcccgcaccg aaggcgtgcc gctgttcatt caagagctct cccacgtgct gcgcgacacc
    13621 agcctggtgg aaaccgacgg ggtgtggagc ttcaagcccg gcgcccaggt gcgcgccatc
    13681 cccatcaccc tgcgcgaatc gctgcacagc cgttttgacc aactgggccc ggcgcgcgac
    13741 attctgcaac tggccgccac cattggccgc gaggtggacg gcggcttgct gcgcgtgtgc
    13801 gctgctgaag cggggccgga tgcgctggcg ctggcgctgg atattctggt ggacgccggc
    13861 ctgctggcgc gcgccgacga cgaccgctat gtgttccgcc acgcgctgat tcgcgacgcc
    13921 gcttacgaca tcatgctgat cagccagcag cggcggcgcc atgaagtggt ggcgcaggcg
    13981 attgtcgagc acgacccaca gcaactgagc gacgcgccgg gctcggtggc tcaccacttc
    14041 gcccgcgccg aacactaccc gcgcgctgtc gagctgggcc tggcgcagct gcggctgacc
    14101 cagcaccgtt cgctgaacga cgaaaccatc gcctatgccg atcaggtcaa cgaatggatc
    14161 aaccggctgg agggccgcgc ccagctggag gcccggctgg agctcaatgg ctgcgtcacc
    14221 caggcgctga tgaacaaata tggctgggcg catccgcagg tggcggaaaa aatcgccctg
    14281 tcggaaagca ttctggcgca agacatttcg gctgaactgc gtttgcaaca tttgtggact
    14341 acgctgacct ttcaccatgt ggccagcaat cgcgccgacg tcatgcagct ggcgcaagcc
    14401 atgctgcagc acggccaggc gcgcgacgac agccatgctt tggtgtcggc gcgcttgtgg
    14461 ctgggcttgg cgcagtattc tgaaggccgt tttgatgatg ccgagcgcga attgtccgcc
    14521 gccatcgacg cgcatgacca tgaggcgcac gccggccacg ccgtgcgctt tggcctggac
    14581 accttggtgt ggtcgcgcgc ggcgcgcgcg ctggtgcgct gggggctggg ctacgaacaa
    14641 tccgcccgcg acgacgcccg cgtggcggtg gcgcgcgcgc gccagctggc gcatgcgccg
    14701 tcgctgggca ttgccttgct ctatctgggg ctttttgagc tgggacgcga tgacgccacg
    14761 gccgcgcaag cggtgtgcga tgagttgatc gagctggcgc atcgttatgg cctgccggcg
    14821 tttttgggtt acgcccagat catccgcagc tgggccgtgg gcgacacggc gcacgccgat
    14881 gccggcattc aggcgctgtg gagcatcggc tgccgctact gccagaccta ttaccgcgct
    14941 tttgccggcc acaccctggc ccgggccggc gatctggccg gcgcgctggc gcggctggac
    15001 gacagcctgt cgctggtgga gccgctggcc gagcgcctgc atgaatcaga actttactgg
    15061 cacaaggcgc gctatctgcg cgccagcggc gctgaggcca gccaagtgcg cgccgccgcc
    15121 gccgccgaca gcgctcgcgc tggcggcaaa tgccggaccg aagca
//
LOCUS       DAOPYX010000033        15086 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_4272_length_15086_cov_9.526113, whole genome shotgun sequence.
ACCESSION   DAOPYX010000033 DAOPYX010000000
VERSION     DAOPYX010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 15086)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15086)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15086
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4272_length_15086_cov_9.526113"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            141..971
                     /locus_tag="PKN55_03025"
     CDS             141..971
                     /locus_tag="PKN55_03025"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNX70249.1"
                     /translation="MPADTGPQRQPPTLNSLPRPVFSRHEALAAGAWTAEHSHPWGQL
                     SYASQGVLGVRTPAGDHMAPPRFAVWTPPGVRHQLFNRRRAQMHSLYIAPEASILPAE
                     CCVLQVSPLARELILRFASLPVEYDQAGAPGRLVAVLLDELAGLPRAAFGLPLPHDRR
                     LLQICAALEAQPDDARTLADWAGVVGASERTLARRFVQETGLGFGQWRQRMRLLLSLD
                     ALQAGDSVTQVALAHGYDSPSAFIAAFRSAFFCTPGALLRQQDPDASARVAHKNLPLA
                     "
     gene            1345..2178
                     /locus_tag="PKN55_03030"
     CDS             1345..2178
                     /locus_tag="PKN55_03030"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNX70250.1"
                     /translation="MSHYQRIACLCTEAVDTLYALGAEEHIAGISGFTVHPPRAREEK
                     PKISGFSSGKIERILSVEPDLVVGYSSLQAELCKDLSKAGVEVLLFNHRTISGILRMV
                     RVLAALVEKEKQGNLLEITLHTRLDVARTQAKLWDLAGWRRPVVYFEEWHEPLTTGIG
                     WVSELIQLAGGVDAFAELSQGNSAKERKIDDAQEVIRRQPDIIIGSWCGRAFDREALM
                     ARPGWADIPAVRMAEDGMVCEIKSSDILAPGPAAIQHGLSQLQQLIGRWHARHHGTLA
                     E"
     gene            2175..3632
                     /locus_tag="PKN55_03035"
     CDS             2175..3632
                     /locus_tag="PKN55_03035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011657662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyric acid synthase"
                     /protein_id="HNX70251.1"
                     /translation="MTRTDFPFSMLMVQGCTSDAGKSTLVAGLCRLLARDGVAVAPFK
                     PQNMALNSAVTVDGGEIGRAQALQALAAGLPPHSDFNPVLLKPSSDIGAQVIIHGRVR
                     ADMSARDYHRYKTTAMQAVLESFARLRQRYACVLVEGAGSPAEINLRARDIANMGFAE
                     AVDCPVILVADIDRGGVFAHLTGTLDCLSASERARVVGFVINRFRGDISLLQGGLDWL
                     EAKTGKPVLAVLPYLHGLMLDAEDAIQAEQRGEGLNVVVPVLPRISNHTDFDALRAHP
                     QVKLCFVGPDAPKPPADLVILPGSKHTRADLAFLQAQGWPDYLRRHLRYGGKLLGICG
                     GYQMLGRSVADPDGHEGSPGVSAGLGWLDADTTLAANKQLREVSGRCLFAEAPVRGYE
                     IHLGHTDGPDCRQRPAFALADGRLDGAMSADGQILGSYLHGLFDHPEAGQALLRWAGL
                     GDAQQVDLAALREASLERLADACQPLYQRLRSLNR"
     gene            3756..4883
                     /locus_tag="PKN55_03040"
     CDS             3756..4883
                     /locus_tag="PKN55_03040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX70252.1"
                     /translation="MRIVNTDITMQSERRASRSARVVNTRLEALVVPRSPMAGTLSPL
                     SEPVTLSEEGKKLAEQARQQIQDHNKASANKVSAETEEAALSPGMAILRSILETVFGV
                     KVKLFNPRDLDPKTGQPTPDAAAGQANPPAAPVADLPNQEPTIEQLIQTQSTGASALR
                     YTTTRYSEEESLDWQASGVVQTADGQTLSFELSLGLSRSAQWEETQVKRIGPPPKDPL
                     VINFAGTAAQLGAGRRTLDLDQDGENETFSMLASGSGFLALDKDGDGKVSDGGELFGT
                     QSGNGFADLAQYDEDGNGWIDEGDTAFAQLKIWAQGEHGQDQLLSLTEAGVGALFLGS
                     QSTEFSLKDNQQQLQGQVRQTGVFLRENGTAGTLQQIDLAV"
     gene            5034..5402
                     /locus_tag="PKN55_03045"
     CDS             5034..5402
                     /locus_tag="PKN55_03045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012698078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2325 domain-containing protein"
                     /protein_id="HNX70253.1"
                     /translation="MNATQAMLVGADTLGNIPAVLSGYGIEIHRHLTGRNSAHQRKQD
                     RLPAGTDLLILFTDFLGHNVMKHFRSLAAQENIRFIACRRSVCALQQSLDSAGFASAD
                     CQRCQANDCVQRKGKSRANA"
     gene            5655..6980
                     /locus_tag="PKN55_03050"
     CDS             5655..6980
                     /locus_tag="PKN55_03050"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNX70254.1"
                     /translation="MKHAPLFLALTLLAPPALAESLDAAWAAALAANPALQASEANTQ
                     ASRHLVNSAKANRWPTLSAGAVWARHQTMPKVQTDLTGLTSGLAGLLPPMALARLPGS
                     VAQPVADDKGWSANATVSLPVFTSGKITAGIRAAEAGVEAAEASETRARQDLKLAVAE
                     AYLNVLRAGEALKVANQLVEALAAHQRDVGQFFQQGLVARSDVLAVEVALADARQKHI
                     QARHAVSLGRSAYNRLLARPLSTPVSLDEVNYPRQAEPLAALQDNAARQRPELEELTA
                     QAQALHAQARAIRAEDGPQVALFAAHTYVQNSYLTREHVNSAGVMLRWNVLDAGIHRE
                     RAAAVAVKADALRVQRNDLASQVALQVEQAWSLQDEAAERREVARAALALADEHLKIQ
                     LDRYANGLAIQTEVLDAQTRRADAARNLFNARYDHALAVLRLKRATGEL"
     gene            6981..8003
                     /locus_tag="PKN55_03055"
     CDS             6981..8003
                     /locus_tag="PKN55_03055"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNX70255.1"
                     /translation="MARPSRPLLIALLLSLAMLAAAGLYWRAQRGKLPEGLVQANGRI
                     EGDHVLVSGKFAGKLARVLVREGDSVSAGQVLAELDDSQIRAKVAQAEASVAALQAQL
                     KVAEQQVPLAVASARAARERAAAASAQAARDAERFDALLERGSVDRRRSEQMRLAATA
                     AATQLTQAEQQVREAELGPDRVAALRAQLGQAEAALAEARAVHNDLTLRAPSPGVVVS
                     KLKQAGETVAAGAPVFDLVDLDRLYLKAYIPENLIGKVRLGQPARLYVDAFPDAAFAA
                     QVATIASRAEFTPKEVQTADERVKLVYAVRLNLSDNRERKLSPGLPADAVIRWDSAVD
                     WQKPRW"
     gene            8012..9946
                     /locus_tag="PKN55_03060"
     CDS             8012..9946
                     /locus_tag="PKN55_03060"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNX70256.1"
                     /translation="MSELPPLIAARGLVKRYGATLAVNALDLSVARGELFGLIGPDGA
                     GKSSVMKAIAGVLTFDGGELAVFGQSLVSDAACEAAKARLGLMPQGLGQNLYADLSVE
                     ENLDFFARLRLVPPDVAAARKDKLLEMTRLARFRQRPMKNLSGGMKQKLGLVCTLIHA
                     PELVILDEPTTGVDPVSRRDFWAILAELLAEEQMTALVSTAYMDEASRFHRVALMHAG
                     QALAVGRPSELLAHAPGCMVTVHTEAQWPALAALRPQFAQLGALGNALQVYADGGDEA
                     QALAAVDACLRPALAADSYQLEAGEPELEDVFIAMLRQRGERLPDSQALTAAAPARAS
                     GGEPVIEARALTRDFAEFRAVDAVSFQVQPGEIFGLLGANGAGKTTVIKMLTGILPPS
                     SGVGRVAGVDMRGAGRAIKERIGYMSQAFSLYLDLTVSENIRLYAGIYGLNRARTQAR
                     LDWIVDMAGLHGFEDALAASLPMGLRQRLALGCALVHQPQVLFLDEPTSGVDPLGRRQ
                     FWDILFTLARRDGVALLVTTHAMSEAEHCDHLALMFAGRLVADASPAAMKRELTAQRG
                     PLWDIRCADPAAALARLRAAGVAASAYGNRLHLFAREDELHARVRPLLGADARCQLRP
                     VSMEDVFVERVTALEAEKPA"
     gene            9943..11094
                     /locus_tag="PKN55_03065"
     CDS             9943..11094
                     /locus_tag="PKN55_03065"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNX70257.1"
                     /translation="MNRHRILAVASKEWREILRDRLFFALAFIVPAFLMLLFGYGLSL
                     DVDHLPLGVIDYDHSEPSRAYTERFSASQYFTFKGYLRDERQAGELLSSNQLRAVIII
                     PPRFGELVQSGQPAQTQTLLDGTFPSRALIAKGYVTAINAAASLDNLARHAGQLGATP
                     DEARRALQPVRLEVRYLYNQSISSIWGLAPKLIMFILIASPPFLTALGVVREKERGSI
                     FNLYASNTSRGEFLLGKLAPYVAISTLNILILWALAVWLYGVPFKGDPLFFLLASVVY
                     VLCTTGIGLLVSVLVRTQMAAMIVTLIVTLVPAILYSGFIIPIPSLKPVAYVVAHLMP
                     AVYYTRIAVGCFLKGVGLTALWPELLILAGYACALFALGYRLFTKRPSA"
     gene            11091..12230
                     /locus_tag="PKN55_03070"
     CDS             11091..12230
                     /locus_tag="PKN55_03070"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNX70258.1"
                     /translation="MNRDDLALWALRLRVMLGKELRQLLRDRVLLAFIVYAFSVDIFL
                     AASGVSLQLNRAATVVQDFDHSTASRELSSRFVSPYFRVDGQISHDSEAQARLDDGRA
                     MLALTIPPGFQADLQRGQPTAVQLQVDATNSVLGFLASSYASLITSRFGLEQAASALG
                     ASGAALPVIDNQVRVWFNPNQLDSWFMGITELLNIVTVFSILLPAAAMVREKERGTVE
                     QLLVSPLSPFQIVFPKVLAMTLVIVLGSALALFGVLQLGFAVPVRGSAVLFLLLTALY
                     VFTTAGLGLAAATLARNLAQVGMLSILILAPMIFLSGAWTPPEAMPAWLRALTYLSPM
                     HYYIDAAFGVLLKGQGALELWRSLLGMTLIGVTVFVWGLRRVRMG"
     gene            12402..14918
                     /locus_tag="PKN55_03075"
     CDS             12402..14918
                     /locus_tag="PKN55_03075"
                     /inference="COORDINATES: protein motif:HMM:NF013727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HNX70259.1"
                     /translation="MSFLKKRKPAAVRNDAPALPPKLSRLLHLATGLALTLGVLLLVA
                     ALASYSPDDNAWSHSADLPYTHNWMGAPGAWLSDILLFVFGLSAWWLPLYAALMVWFG
                     QRDDERFSPELLSWAGLAVYGGFTVLLLSSSAFEAIRLYSLDVALPLSAGGIVGDMLG
                     KTLHRALGFNGASLLLMIAMLVGFSAFTHVSWLNLMEAIGAGVEYLALAVRRLWQRSL
                     GEEMLDTPHAGSPASQAPAAEVASEPVADSPAEEAPAAPLASGSRIEPRLGDEPALAD
                     EHPAQADIAAQADSPADHDAATASAASHEPPVLVLDSEDEAIPAADAPAPSLPAAQPS
                     TVPAQPPRRHGPPRLLLPSAPLPPDTLLQRLPPDAATDEETVDKARVEQALLALGVPA
                     TVLSAHVGPVVIRYEFEAGDAIAPERRATLARELTQALAVDSLRLLDSVPGKSCMALE
                     HPRRQRQPVLLSVALRSPAWRNSPSRLTLALGVDSSGQPMTADLARMPHVLLAGAPGS
                     GKTTALHAMLLSLLYKATPEEARLLLIDPEHSLAAYRELPHLLAPVVSDMQQAALALG
                     WCMSEMERRYQLMARLGVRNLASYNQKLREADSRDERIADPFSEPDDLKPLELMPAIV
                     VVVNELAELMAQGGKQVEERLARLAQKARAAGIHLVLATGRASAEVITGLIKANIPTR
                     MAFQVASKVDSRLILEQMGAETLLGQGDMLYKPPGSDYALRVHGADVSPADISQVTEF
                     VRRTGKPDYVDGLLEGAPSVGLPRPHRVADPSEVALDPLYDDAVARVRQLDKATVMLL
                     QSEFNIGATRAIRLLDSLELTGVISAADANGHRKVLAVSL"
BASE COUNT         2537 a         4929 c         4906 g         2714 t
ORIGIN      
        1 gtgttcattt catgaaaatg gtcatcaact gctctgcgga cttgagctga tatccattca
       61 tgcgattgcc ctgtaccgcc atctgtgttg gcggtttctc gtgataaagt caaacactat
      121 cgagaaactg ccaaatcacc atgcctgctg acaccggccc acagcgccag cctcccacct
      181 tgaacagctt gccgcgcccg gtgttcagcc gccatgaagc actggctgcc ggggcgtgga
      241 ctgccgagca cagccaccca tgggggcagt tgtcctacgc cagtcagggc gtgctggggg
      301 tgcgcacgcc agcgggcgac catatggcgc cgccacgatt tgccgtgtgg acgccgccgg
      361 gagtgcgcca tcagctattc aaccgccgcc gcgcgcagat gcacagcctg tacatcgcgc
      421 ccgaggcctc aatcctgcca gccgaatgct gcgtgttgca ggtcagcccg ctggcgcgcg
      481 agttgatttt gcgctttgcc agtctgccgg tggaatacga ccaagccggt gcgcccggcc
      541 ggctggtggc ggtgttgctg gatgagttgg ccggcttgcc gcgtgcggca ttcggcctgc
      601 ccttgccgca cgaccgacgc ttgctacaga tatgcgccgc gctggaagct cagccggatg
      661 acgcacgcac gctggcggac tgggccggcg tggtgggggc ttccgagcgc acgctggcgc
      721 ggcggtttgt gcaggaaacc ggcctgggct ttggccagtg gcgccagcgc atgcgcttat
      781 tactgtcact ggatgccttg caagccgggg acagcgtcac ccaggtggcg ctggcgcacg
      841 gctatgactc tccatccgcg tttattgcgg cttttcgcag tgcatttttt tgcacgcctg
      901 gtgcgctgct gcgccagcaa gaccctgatg cctcggcgcg ggttgcgcac aaaaatttgc
      961 ctttggcata gatggcgggc ggtgtcagcc agacagaccc tcaccccgcg ctgggcggca
     1021 gcacaggctg gcagccaagt gtttacattt attactcttg tcccccactg actcgtcact
     1081 gagtcaggtc agttctttat caggaatttc attccattgg aattgccttt cgcttaggtg
     1141 aagttcaggt gacttaagca tagccagtgc ggtcaacttg cattaccatg acattcatac
     1201 acggataaaa aatccgtatg gcatttttat aggcatgtgt cacatcttga tagtgcgcgc
     1261 gctttgcagc cgtctgccgg tggcgccccc cgaggcgcgc acattgatga ccccagtaag
     1321 aaagaggaaa aggcccaatc cgtcatgagc cattaccaac gtattgcctg cttgtgcacc
     1381 gaagcggtgg acacgctgta tgccttgggc gcagaagaac acatcgccgg catttccggc
     1441 ttcaccgtgc acccgccgcg cgctcgcgaa gaaaaaccca agatcagcgg gttttccagc
     1501 ggcaagatcg agcgcattct gtcggtcgag cctgatttgg tggtgggcta ctccagcctg
     1561 caggccgagt tgtgcaagga tttgtccaag gccggcgtcg aggtgctgct gttcaaccac
     1621 cgcaccattt ccggcatcct gcgcatggtg cgggtgctgg ccgcgctcgt cgaaaaagaa
     1681 aaacagggca atctgctgga aatcaccctg cacacccggc tggacgtggc gcgcacccag
     1741 gccaagctgt gggatctggc cggctggcgt cggccagtgg tgtactttga agaatggcac
     1801 gagccgctga ccaccggcat tggctgggtg tccgagctga tccagctggc cggcggggtg
     1861 gatgcctttg ccgagttgag ccagggcaat agcgccaaag agcgcaagat cgacgatgcg
     1921 caagaagtga ttcgccgcca gccggacatc atcatcggtt cgtggtgcgg acgcgcgttt
     1981 gaccgtgagg cactgatggc gcggccgggg tgggcggaca tcccggcggt gcgcatggcc
     2041 gaggacggca tggtgtgcga gatcaagtcc agcgacattc tcgcccccgg cccggcggcc
     2101 attcagcacg gcctaagcca gctgcaacag ttgattggcc gctggcatgc gcgccatcac
     2161 ggtacactgg cggaatgacc cgcacagact ttccgttttc catgctgatg gtgcaaggct
     2221 gcacctcgga cgccggcaaa agcacgctgg tggctggcct gtgccggctg ctggcgcgcg
     2281 acggcgtggc cgtggcccca ttcaagccac agaatatggc gctgaacagc gctgttaccg
     2341 tcgatggcgg cgaaattggc cgcgcccagg cgctgcaagc gctggcggcc ggtttgccgc
     2401 cgcattccga cttcaacccg gtgctgctca agcccagcag cgacatcggc gcgcaagtga
     2461 tcatccacgg ccgcgtgcgc gccgacatgt ccgcccgcga ctaccaccgc tataaaacca
     2521 ccgccatgca agcggtgctg gaatcctttg cccgcctgcg ccagcgctac gcctgcgtgc
     2581 tggtggaagg cgccggcagc ccggcggaaa tcaatctgcg cgcgcgcgac atcgccaata
     2641 tgggctttgc cgaggcggtg gactgcccgg tgattctggt ggccgacatc gaccgtggcg
     2701 gcgtgttcgc ccacctcacc ggcacgctgg actgcctgtc cgccagcgag cgcgcccgcg
     2761 tggtcggctt tgtcatcaac cgttttcgtg gcgacatcag cctgctgcaa ggcgggctgg
     2821 actggctgga agccaaaacc ggcaagccgg tgctggcggt gctgccctat ctgcacggcc
     2881 tgatgctgga cgccgaagac gccatccagg ccgagcagcg cggcgaaggg ctgaatgtcg
     2941 tcgtgccggt gctgccgcgc atcagcaatc acaccgactt tgacgccttg cgcgcccacc
     3001 cgcaggtcaa gctgtgcttt gtcggcccgg acgcgcccaa accgccggct gatctggtga
     3061 ttctgcccgg cagcaagcac acccgcgccg acctggcgtt tttgcaggcg caaggctggc
     3121 cggactatct gcgccgccat ctgcgctacg gcggcaagct cttgggcatt tgcggcggct
     3181 accagatgct gggccgcagc gtggccgacc cggacggcca cgaaggctcg cccggcgtca
     3241 gcgccggctt gggctggctg gacgccgaca ccacgctggc ggccaacaag caattgcgag
     3301 aagtcagcgg ccgttgtctg tttgccgagg cgccagtgcg cggctacgaa attcacctgg
     3361 gccacaccga cggcccggat tgccgccagc gcccggcctt tgccctggcc gatggccgcc
     3421 tggacggcgc catgagcgct gatgggcaga ttctgggcag ttatctgcat ggcctgttcg
     3481 accaccccga agccggccag gcgctgctgc gctgggccgg gctgggcgac gcccagcagg
     3541 tggatctggc cgcgctgcgc gaagccagcc tggaacggct ggctgacgcc tgccaaccct
     3601 tgtatcagcg cttgcgcagc ctgaatcggt aaaactacgt ggcaaacatg gccgatagcc
     3661 gcaacagcat cttgatttaa agaagttttt caagattctg cgccagcgga cgataagtat
     3721 gtcatcttta ctttattgtc tgccggaggc ccgccatgcg cattgtcaac accgacatca
     3781 ccatgcaatc cgaacggcgc gccagccgct cggcgcgcgt ggtgaacaca cgtctggagg
     3841 ccctggtggt gccgcgcagc ccgatggccg gcacgctgtc gcccttgtct gagccggtca
     3901 cgctgagcga agaaggcaaa aaactggccg agcaagcgcg ccagcagata caggaccaca
     3961 acaaggcatc ggccaacaag gtcagcgccg aaaccgaaga agccgcgctc agccccggca
     4021 tggcgattct gcgttcgatt ctggaaaccg tgtttggcgt caaggtcaag ctgttcaacc
     4081 cgcgcgacct cgatccaaaa accggccagc ccaccccaga cgcagccgct ggccaggcca
     4141 atccgccggc cgccccggtg gccgatctgc ccaatcaaga acccaccatc gagcaattga
     4201 tacaaacgca aagcacaggc gcctctgccc tgcgctacac caccacccgc tacagcgaag
     4261 aagaatccct ggattggcag gccagtggcg tggtgcaaac cgccgatggg caaacgctga
     4321 gctttgaact cagcctgggt ttgtcgcgta gcgcgcaatg ggaagaaacc caggtcaagc
     4381 gcattggccc gccaccgaaa gacccgctgg tgatcaactt tgccggcact gccgcccaat
     4441 tgggcgctgg ccggcgcacg ctggacctgg atcaggacgg cgagaatgaa accttctcga
     4501 tgctggcttc cggcagcggc tttctggcgc tggataaaga cggcgacggc aaggtcagcg
     4561 atggcggcga gttgtttggc acacaaagcg gcaatggctt tgccgacctg gcccagtacg
     4621 acgaagacgg caatggctgg atcgacgaag gcgacacggc atttgcccag ctgaaaatct
     4681 gggcgcaggg cgaacacggc caggatcaac tgctcagcct gaccgaagct ggcgtgggcg
     4741 cgctgttttt gggcagtcaa tccaccgagt tttccttgaa ggacaatcag cagcagttgc
     4801 aaggccaagt gcggcaaacc ggggtgttcc tgcgtgaaaa cggcacagcg ggcacgctgc
     4861 aacagattga tttggccgtg taaaccgcca ttgggcgata cctctccatc agccggcgcg
     4921 ccactgggcg cggccggctg atttttttgc cacaccccgt gtttttacgc ttgcaaaatg
     4981 gcttgggctg ccgttaagat gataacgaat atcatttaca tgagtagctt gccatgaacg
     5041 ccacccaagc catgctggtc ggagccgaca ccctgggcaa tatccccgcc gtgctcagcg
     5101 gctacggcat tgaaattcac cgccacctca ccggccgcaa cagcgcccac cagcgcaaac
     5161 aggatcgcct gccggccggc actgacttgt tgatcttgtt cactgatttt cttgggcaca
     5221 atgtgatgaa gcattttcgc tcgctggctg cgcaagaaaa catccgcttt atcgcctgcc
     5281 gccgctcggt gtgcgcgctg caacaatcgc tggacagcgc cggttttgcc agcgccgact
     5341 gtcaacgctg ccaggccaat gactgcgtac agcgcaaggg caagagccgg gcaaacgcct
     5401 gaggccgtgg gctcagcaaa ctttcctggt gtgcttgccg tacacgcttg tgccgtcagt
     5461 ggccgcttgc cgatgcggtt ttcacgcccc gtccttggac ggggcgtgcg cttttctgcg
     5521 gctcgacgcc ctgacctgct tcatggtagc gtgctggctc taaaaaaata tgcggtccgc
     5581 ttgccggtgc tatttttcaa acgtatgctt gagcgcattc gtgtcgcgcg acctcaaact
     5641 tctgcaaagt cagcatgaaa cacgcccctc tttttctggc cctgaccctg ctggcgccgc
     5701 cggcgctggc cgaatcgctg gacgccgcct gggccgccgc actggcggcc aacccggcgc
     5761 tgcaagccag tgaagccaac acccaggcca gccgccatct ggtgaacagc gccaaggcca
     5821 accgctggcc cacgctgtcg gccggcgcgg tgtgggcgcg ccaccaaacc atgcccaagg
     5881 tgcaaaccga cctcaccggg ctgaccagcg gcctggccgg cctgctgccg ccgatggcgc
     5941 tggcgcgcct gcccggctcg gtggcgcagc cggtggccga tgacaagggc tggagcgcca
     6001 acgccaccgt cagcctgccg gtgttcacca gcggcaagat caccgccggc attcgcgccg
     6061 ccgaagccgg cgtcgaggcc gccgaggcca gcgaaacccg cgcccggcaa gacctcaagc
     6121 tggcggtggc cgaggcgtat ttgaatgtgt tgcgcgctgg cgaggcgctg aaagtggcca
     6181 atcagctggt ggaagcgctt gccgcccatc agcgcgatgt cgggcagttt tttcagcaag
     6241 ggctggtggc gcgcagcgat gtgctggcgg tggaagtggc gctggccgac gcccggcaaa
     6301 agcatatcca ggcccgccac gccgtcagcc tgggccgcag cgcctataac cgcctgctgg
     6361 cgcggccgct gtccaccccg gtcagcctgg atgaggtgaa ttatccgcgc caggccgagc
     6421 cgctggccgc cctgcaagac aacgccgccc gccagcgccc cgagctggaa gagctcaccg
     6481 cccaggcgca ggcgctgcat gcccaggcgc gcgccatccg cgccgaagac ggcccgcagg
     6541 tggcgctgtt tgccgcgcac acctatgtgc aaaactccta cctgacccgc gagcatgtca
     6601 attccgccgg ggtgatgctg cgctggaatg tgctggacgc cggcatccac cgcgagcgcg
     6661 ccgccgccgt ggcggtgaaa gccgacgcgc tgcgcgtgca gcgcaacgac ctggccagcc
     6721 aggtggcctt gcaggtggag caagcctggt ccttgcagga cgaagccgcc gagcggcgcg
     6781 aggtggcgcg cgccgcgctg gcgctggccg acgaacacct gaaaatccag ctcgaccgct
     6841 acgccaacgg cctggccatc cagaccgaag tgctggacgc acaaacccgc cgcgccgacg
     6901 ccgcgcgcaa tctgtttaac gcccgctacg accacgcgct ggcggtgctg cgcctgaagc
     6961 gcgccaccgg agaactgtaa atggcccgtc cttcccgtcc gctattgatt gccctgctgt
     7021 tgtcgctggc catgctggcg gcagccggcc tgtactggcg cgcccagcgc ggcaagctgc
     7081 ccgaaggcct ggtgcaggcc aatggccgca tcgagggcga ccatgtgctg gtgtcgggca
     7141 agtttgccgg caagctggcg cgcgtgctgg tgcgcgaggg cgacagcgtc agcgccggcc
     7201 aggtgctggc cgaactggac gacagccaga ttcgcgccaa agtcgcccag gccgaagcca
     7261 gcgtggccgc cttgcaggcg caactgaaag tggccgaaca acaagtgccg ctggcggtgg
     7321 cctcggcgcg cgccgcccgc gagcgcgccg ccgccgcctc ggcccaggcc gcgcgcgacg
     7381 ccgaacgctt tgatgccctg ctggagcgcg gttcggtgga ccgccgccgc agcgagcaaa
     7441 tgcgcctggc cgccaccgcc gccgccaccc aactgaccca ggccgaacag caagtgcgcg
     7501 aagccgaact cggccctgac cgggtggcgg cgctgcgcgc ccagttgggt caggccgaag
     7561 ccgcgctggc ggaagcgcgc gccgtgcaca acgatctcac cctgcgcgcg cccagccccg
     7621 gcgtggtggt cagcaagctc aagcaagccg gcgaaaccgt cgcggcgggc gcgccggtgt
     7681 ttgacctggt ggacctggat cgcctgtacc tgaaagccta tatcccggaa aacctgatcg
     7741 gcaaagtgcg tctgggccag ccggcgcgcc tgtatgtgga cgccttcccc gacgccgcct
     7801 ttgccgccca ggtggccacc atcgccagcc gcgccgaatt cacccccaaa gaggtgcaaa
     7861 ccgccgatga gcgggtcaag ctggtgtacg ccgtgcggct gaacctcagc gacaaccgcg
     7921 agcgcaagct cagccccggc ctgccggccg acgcggtgat tcgctgggac agcgccgtag
     7981 actggcaaaa gccgcgctgg tgagccaggc aatgagcgaa ctgcccccgt tgattgccgc
     8041 gcgcggcctg gtgaaacgct atggcgccac gctggcggtg aacgcgctgg atttaagcgt
     8101 ggcgcgcggc gagctgttcg ggctgattgg cccggacggc gccggcaaat ccagcgtgat
     8161 gaaagccatt gccggcgtgc tcacctttga cggcggcgag cttgctgtat ttggccaatc
     8221 cttggtcagc gatgccgcct gcgaggcggc caaggcgcgg ctggggctga tgccgcaggg
     8281 cctgggccag aacctgtacg ccgatttgtc ggtagaagaa aacctggatt tttttgcccg
     8341 cctgcgcctg gtgccgcccg atgtcgccgc cgcgcgcaag gacaaactgc tggaaatgac
     8401 ccggctggcg cgctttcgcc agcggccaat gaaaaacctc tccggcggca tgaaacaaaa
     8461 actcggcctg gtgtgcacgc tgattcacgc ccccgagctg gtgattctgg acgaacccac
     8521 caccggcgtg gacccggtct cgcggcggga tttctgggcg attctggccg agctcttggc
     8581 cgaagaacag atgaccgcgc tggtgtccac cgcctatatg gacgaagcca gccgctttca
     8641 ccgcgtggcg ctgatgcacg ccggccaggc gctggcggtg ggccgcccca gcgagctgct
     8701 ggcgcacgcc cccggctgca tggtgaccgt gcacaccgag gcgcaatggc cggcgctggc
     8761 ggcgctgcgc ccgcagtttg cccaactggg cgcgctgggc aatgccttgc aggtgtacgc
     8821 cgacggcggc gacgaagcgc aggcgctggc cgccgtggac gcctgcctgc gcccggcgct
     8881 ggcggcggac agctatcagc tggaagccgg cgaacccgag ctggaagacg tgttcatcgc
     8941 catgctgcgc cagcgcggtg agcgcctgcc cgacagccag gcgctcaccg ccgccgcccc
     9001 ggcgcgcgcc agcggcggcg agccggtgat tgaagcgcgg gcgctgaccc gcgactttgc
     9061 cgagtttcgc gcggtggacg cggtgagttt tcaggtgcag ccgggcgaga tttttggcct
     9121 gcttggcgcc aacggcgccg gcaaaaccac ggtgatcaag atgctcaccg gcatcctgcc
     9181 gcccagcagc ggcgtgggcc gggtggccgg ggtggacatg cgcggcgccg ggcgcgccat
     9241 caaagaacgc atcggctata tgtcgcaggc gttttccctg tatctggacc tcaccgtcag
     9301 cgagaacatc cgcctgtacg ccggcattta tggcctgaat cgcgcgcgca cccaggcgcg
     9361 gctggactgg attgtggaca tggccggcct gcacggcttt gaagacgccc tggccgccag
     9421 cctgccaatg ggcctgcgtc agcggctggc gctgggctgc gcgctggtgc atcagccgca
     9481 ggtgctgttt ctggacgaac ccacctcggg ggtggaccca ctgggccgcc gccagttttg
     9541 ggatatttta ttcaccctgg cccgccgcga cggcgtggcg ctgctggtga ccacccacgc
     9601 catgagcgag gccgaacact gcgaccacct ggcgctgatg tttgccggcc gcctggtggc
     9661 cgacgccagc ccggcggcga tgaagcgcga actcaccgcc cagcgcggcc cgctgtggga
     9721 catccgctgc gccgaccccg ccgccgccct ggcgcggctg cgcgccgctg gcgtggcggc
     9781 cagcgcctac ggcaaccgcc tgcacctgtt tgcccgcgaa gacgaactgc acgcccgcgt
     9841 gcgcccgctg ctgggcgccg acgcccgctg ccaactgcgg ccggtgagca tggaagacgt
     9901 gtttgtcgag cgcgtcaccg cgctggaagc cgagaagccc gcatgaaccg acaccgcatc
     9961 ctcgccgtgg ccagcaagga atggcgcgaa atcctgcgcg accggctgtt ttttgccctg
    10021 gcttttatcg tgccggcctt tttgatgctg ctgtttggct atggcctgtc gctggatgtg
    10081 gatcatctgc cgctgggggt gattgactat gaccacagcg agcccagccg cgcctatacc
    10141 gagcgcttca gcgcctcgca atatttcacc ttcaaggggt atctgcgcga tgagcgccag
    10201 gcgggtgagt tgctgtccag caaccagctg cgcgcggtga tcatcattcc gccgcgcttt
    10261 ggcgagctgg tgcagtccgg ccagccggcg cagacgcaaa ccttgctgga cggcaccttt
    10321 cccagccgcg cgctgatcgc caagggctat gtcaccgcca tcaacgccgc cgccagcctg
    10381 gacaatctgg cccgccacgc cggccagctg ggcgccacgc cagacgaggc gcggcgcgcg
    10441 ctgcaaccgg tcaggctgga ggtgcgctat ctgtataacc agagcatttc cagcatctgg
    10501 gggctggcgc ccaagctgat catgttcatc ctgatcgcct cgccgccgtt tctgactgcg
    10561 ctgggcgtgg tgcgcgagaa agaacgcggt tcgattttca atctgtacgc ctccaacacc
    10621 agccggggcg agtttttgct gggcaagctg gcgccgtatg tggcgatttc cacgctgaac
    10681 atcctgattt tgtgggcgct ggcggtgtgg ctgtatggcg tgccgttcaa gggcgacccg
    10741 ctgttctttt tgctggccag cgtggtgtat gtgctgtgca ccaccggcat cgggctgttg
    10801 gtgtcggtgc tggtgcgcac gcagatggcg gcgatgatcg tcaccctgat cgtcaccctg
    10861 gtgccggcga ttttgtattc gggttttatt attccgattc cctcgctcaa gcccgtggcc
    10921 tatgtggtgg cgcacctgat gccggcggtg tactacaccc gcattgccgt tggctgtttt
    10981 ctcaaggggg tggggctgac ggcgctgtgg ccggagttat tgattttggc cggctacgcc
    11041 tgcgccttgt ttgcgctggg ctatcggctg ttcaccaaga ggccatcggc atgaatcgcg
    11101 acgatctggc gctgtgggcg ctgcgcctgc gggtgatgct gggcaaggag ctgcgccagc
    11161 tgctgcgcga ccgggtgttg ctggcgttta ttgtctacgc gttcagcgtg gatatttttc
    11221 tggcggcctc gggcgtcagc ctgcaactga accgcgccgc caccgtggtg caggactttg
    11281 accactccac cgcctcgcgc gagctgagct cgcgctttgt gtcgccgtat tttcgcgtgg
    11341 atgggcaaat cagccacgac agcgaagccc aggcgcggct ggacgacggc cgcgccatgc
    11401 tggcgctgac cattccgccg ggctttcagg cggatttaca gcgcggccag cccaccgccg
    11461 tgcaattgca agtagacgcc accaattcgg tgctgggctt tttggcgtcg agctacgcca
    11521 gcctgatcac cagccgtttt ggcctggagc aagccgccag cgcactgggc gccagcggcg
    11581 cggcgctgcc ggtgattgat aaccaggttc gcgtgtggtt caaccccaat cagctggaca
    11641 gctggtttat gggcatcacc gagctgttga atatcgtcac ggtgttttcc attttgctgc
    11701 cggcggcggc gatggtgcgc gaaaaagaac gcggcacggt cgagcaattg ctggtcagcc
    11761 ccttgtcgcc gttccagatt gtgttcccca aggtgctggc gatgacgctg gtgattgtgc
    11821 tgggcagcgc gctggcgctg tttggcgtgc tgcaactggg gtttgccgtg ccggtgcgcg
    11881 gcagcgccgt gctgtttttg ctgctgaccg cgctgtatgt gttcaccacc gccggcctgg
    11941 gcctggccgc cgccacgctg gcgcgcaatc tggcccaggt gggcatgttg tccattttga
    12001 ttctggcgcc gatgattttt ctttctggcg cctggacgcc gccagaagcc atgccggcct
    12061 ggctgcgcgc gctgacttat ctgtcgccga tgcactatta catcgacgct gcctttggcg
    12121 tgttgctcaa gggccagggc gcgctggagc tgtggcgctc gctgcttggc atgacgctga
    12181 tcggcgtgac ggtgtttgtc tgggggctgc gccgggtgcg catgggttga gcacttttac
    12241 acaattacac aatacgcctg atgttgttgt atggctttgt catttaataa atgctgtatg
    12301 gatggctctg ccccgccaag atttttatcc atcgccctgc cgcctgcgcg ccggggcatt
    12361 ccccgctata atcggcgcgg ttccgacccc agacgcgagt catgtcattt ctgaaaaaac
    12421 gtaagccggc agcggtgcgc aatgatgccc cggcgctgcc ccccaagctg tcccgcctgc
    12481 tgcacctggc cactggcctg gcgctgacgc tgggtgtatt gctgctggtg gccgcgctgg
    12541 ccagttactc cccggatgac aacgcctggt cacatagcgc cgacctgccc tacacccata
    12601 actggatggg cgcgccaggt gcctggctgt ccgacatcct gctgtttgta tttggcctgt
    12661 cagcctggtg gctgccgctg tatgccgcgc tgatggtctg gtttggccag cgtgacgacg
    12721 agcgcttttc gcccgagctg ctcagttggg cgggtctggc ggtgtatggc gggtttaccg
    12781 tgctgttgct gtccagctcg gcatttgagg ccattcgttt gtatagcctg gacgtggcgc
    12841 tgccgctgtc ggctggcggg attgtcggcg acatgctggg caagacgctg caccgggcac
    12901 tgggcttcaa cggcgcttca ctgctgctga tgatagccat gctggtggga ttttccgcat
    12961 tcacccatgt gtcgtggctg aacctgatgg aagccattgg cgccggggtg gaatacctgg
    13021 cgctggcggt gcgccggttg tggcagcgca gcctgggcga agaaatgctg gacaccccgc
    13081 atgctggctc gcccgccagc caagcccccg ctgccgaggt ggccagcgaa cctgtcgccg
    13141 acagcccggc cgaggaggca ccagccgctc cgttggccag cggtagccgc attgagccac
    13201 ggctgggtga cgagcccgcc ctggcggatg agcatccggc acaggcagac atcgctgcgc
    13261 aggcagacag cccggcagat cacgatgcag caaccgccag cgcagccagc cacgagccgc
    13321 cggtgctggt gctggacagc gaagacgagg ccatccccgc cgccgatgcc ccggcgccaa
    13381 gcctgcccgc agcgcaacca tccaccgtac cggcccagcc accgcgccgc catggcccgc
    13441 cccggctgct gctgcccagt gcgcctctgc cacccgatac cctgttgcaa cgcctgccgc
    13501 cagacgcggc caccgacgag gaaacggtag acaaagcccg cgttgaacag gcgctactgg
    13561 ccctgggggt gccggccacc gtgctgagcg cccatgtggg cccggtggtg attcgctatg
    13621 agttcgaagc cggtgatgcc atcgcaccgg aacgccgcgc caccctggcc cgtgaattga
    13681 cccaggcgct ggccgtggat tcactgcgcc tgttggatag cgtgcccggt aaatcctgca
    13741 tggcgctgga gcacccccgc cgccagcgcc agcccgtttt gctgtccgtg gcgctgcgca
    13801 gcccggcctg gcgcaacagc ccctcgcggc tgaccctggc gctgggggtg gacagcagcg
    13861 gccagcccat gaccgccgac ctggcgcgca tgccgcatgt gctgctggcc ggcgcgcccg
    13921 gctcgggcaa gaccacggcg ctacacgcca tgctgctctc gctgctgtac aaagccaccc
    13981 cggaagaagc ccgcctgctg ctgatcgacc ccgagcacag cctggccgcc tatcgtgagc
    14041 tgccgcatct gctggccccg gtggtcagcg atatgcagca agctgctctt gccttgggct
    14101 ggtgcatgag cgagatggag cgccgctatc agctgatggc gcggttgggc gtgcgcaatc
    14161 tggccagtta caaccaaaaa ctgcgcgaag ccgacagccg cgacgaacgc attgccgacc
    14221 cattcagcga gccggacgac cttaaaccgc tggaactgat gccggctatc gtggtggtgg
    14281 tcaatgaact ggccgagctg atggcgcagg gaggcaagca ggtggaagag cgcctggcac
    14341 gcctggcgca aaaagcccgc gcggccggca ttcatctggt gctggccacc gggcgcgctt
    14401 cggctgaggt gatcactggc ctgatcaagg ccaatattcc cacccgcatg gcgtttcagg
    14461 tggcatccaa ggtggatagc cggctgattc tggagcagat gggcgccgaa accctgctgg
    14521 gccagggcga tatgctgtac aagccgccgg gcagtgatta tgccctacgg gtgcacggcg
    14581 ctgatgtcag cccggctgat atcagtcagg tcaccgagtt tgtgcgccgc accggcaagc
    14641 cggattatgt ggatggcctg ctggaaggcg cgccctcggt gggcttgccg cgtccgcatc
    14701 gggtggcgga tccatccgaa gtggcgctgg acccgctgta cgacgatgcc gtggcgcggg
    14761 tgcgccagtt ggacaaggcc acggtgatgc tgctgcaaag cgaattcaac atcggcgcca
    14821 ctcgcgccat ccgcctgttg gacagtctgg agctgactgg cgtgatctcg gcagccgatg
    14881 ctaacggcca ccgcaaggtg ctggccgtca gcctttgacc accgccgggt gcaatgcgcc
    14941 cggccagtac cccgcggatt gaccaacacc aacagcctgc catgtggtca tgggcaggct
    15001 ttttcgtttt cgaacatgtt tagagcggct aacaaaaccc tcctggcgtt gttgcacgac
    15061 cttgccgtac tacccgtact gtctgc
//
LOCUS       DAOPYX010000034        14832 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_4384_length_14832_cov_10.426947, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000034 DAOPYX010000000
VERSION     DAOPYX010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 14832)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14832)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14832
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4384_length_14832_cov_10.426947"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(257..1204)
                     /gene="ispH"
                     /locus_tag="PKN55_03080"
                     /gene_synonym="lytB"
     CDS             complement(257..1204)
                     /gene="ispH"
                     /locus_tag="PKN55_03080"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004283681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HNX70260.1"
                     /translation="MSQPIQVLLANPRGFCAGVDRAIAIVERALAQFGAPIYVRHEVV
                     HNKFVVNNLREQGAIFIEDLADVPPGSTLIYSAHGVPLSVRAEAEQRGLRVFDATCPL
                     VTKVHVEVRRMRDAGQEIVMIGHAGHPEVEGTMGQSPDGMYLVETVADVATLSVRNPQ
                     ALAYVSQTTLSVDETRDIIDALRARFPDIHGPKKDDICYATQNRQDAVKALTAQCDLV
                     IVVGSPNSSNSNRLREVAALRGVTAYMVDNASELKPEWFAQASQVGVTAGASAPDVLV
                     RSVIERIRELGADHVAELPGVEERVRFSLPPGLRERDEG"
     gene            complement(1201..1683)
                     /gene="lspA"
                     /locus_tag="PKN55_03085"
     CDS             complement(1201..1683)
                     /gene="lspA"
                     /locus_tag="PKN55_03085"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018748263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HNX70261.1"
                     /translation="MPEAVRWFVLSALVIVLDQLSKVYFNGAYQYGEVREVIPGLFNF
                     VLVYNPGAAFSFLADAGGWQKFFFTALAFAVSGWLGWQMLRGGQSRMMNLAGALIIGG
                     ALGNVIDRLIHGHVIDFIQVYYQHYYWPAFNLADSAICLGAALMVLDSFRQPATRKPA
                     "
     gene            complement(1676..4546)
                     /gene="ileS"
                     /locus_tag="PKN55_03090"
     CDS             complement(1676..4546)
                     /gene="ileS"
                     /locus_tag="PKN55_03090"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008953258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HNX70262.1"
                     /translation="MSTDSKKYAVNLLDTAFPMRGDLAKREPGWVAQWQAGQRYQALR
                     QRTAGRPKFVLHDGPPYANGDIHIGHAVNKILKDIIIRSKTLAGFDAPYVPGWDCHGL
                     PIEHQIEKLIKGDKKAIEAAPGIHQLIVAYRQQNGLDPKETRLPASVVRKLCRSYAMV
                     QIERQKADFIRLGVLGDWDNPYLTMNYRTEADIVRTLGKIHQNGYLVQGKKPVHWCID
                     CGSALAEAEVEYEDKTSPAIDVGFLVADTARLAAAFGLASLDGQARAVIWTTTPWTLP
                     ANQAVAVHPNLDYQLWATARGALILAAELAEGALKRYNLADGAQLLGQAKGAALDLLP
                     LQHPFQPRQVPVILGEHVGADAGTGLVHTAPAHGLEDFQAGLKYQLAVDNPVGDDGRF
                     IPGTPLFAGLSVWEANPKVLETLTEHDALLASEALKHSYPHCWRHKTPIIFRATSQWF
                     IGMDTVGQDGVALRANAMRAVEHTQFFPAWGRARLEAMIGNRPDWCVSRQRNWGVPMT
                     FFVHKETGALHPRSAELLEQVALRIEQEGIEAWFQLDAAELLGDEASQYRKLTDTLDV
                     WFDSGSTHYAVLRQRDELKWPADLYLEGSDQHRGWFQSSMLTGCATTGRAPYDQLLTH
                     GFVVDGQGRKMSKSLGNVIAPQKINDTLGADILRLWVAATDYSGELAISDTILKGVTE
                     SYRRLRNTIRFLLANLTDFDLARDGVAVDNLLEIDRYALAQTRALQSRLTGDLYPRYA
                     FHHAMQDIVTFCSDDLGAFYLDILKDRLYTTAADSSARRSAQTALWHITRALLLWLQP
                     VLCFTSDEAWAVFSGDAEDSALFHTWHALPEVAGEAELLARWSALRAVRANVNKLIED
                     QRVANVLGSSLQAEVDIVADAELYPLLASLGDDLKFVLIVSKVTLSAGEHTAISVRAS
                     EAQKCERCWHWRDDVGAHAEHPTLCGRCVSNLFGDGEARAHA"
     gene            complement(4543..5475)
                     /locus_tag="PKN55_03095"
     CDS             complement(4543..5475)
                     /locus_tag="PKN55_03095"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003823926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HNX70263.1"
                     /translation="MQVHFGASAARPSACALTIGNFDGVHRGHQAMLACLRERASARG
                     LPTALLTFEPHPREVFARGNPPARLATLRDKVLSLKATGLLDHLYVHRFTPAFAALSA
                     QDFIDQILVAGLGAQYVLIGDDFQFGARRLGNFSTLQACPHFVTEAMPTISVHGERAS
                     SSRIREALAAGDFAHAEALLGRHYQLTGRVMHGKKLGRTLGYPTANVHLPHRKPALAG
                     IFVVEVDTAAGRFGGVASLGKNPTVDQGDDYKLEVHLFDFAGNLYGQLICVRFLAKLR
                     DEQRFDSLAALTAQIDADAAAARHHLSTLQRVSA"
     gene            complement(5595..6050)
                     /locus_tag="PKN55_03100"
     CDS             complement(5595..6050)
                     /locus_tag="PKN55_03100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008953218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HNX70264.1"
                     /translation="MMELRLRAWLARCLPWSRAANALRAPLTLGVRLFLADVFFRAGM
                     TKLADWDVTLALFTDEYQVPVLPPMLAALLATACEILLPMLLSLGLATRVAALGLFAL
                     NLMAVISYPAMPEIARQFHVYWGMLLALLLAYGGGGWSVDRFLLRRLHP"
     gene            complement(6116..6865)
                     /locus_tag="PKN55_03105"
     CDS             complement(6116..6865)
                     /locus_tag="PKN55_03105"
                     /inference="COORDINATES: protein motif:HMM:NF021364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding domain-containing protein"
                     /protein_id="HNX70265.1"
                     /translation="MSALLRWQSQLIDAITAPEAPSPLPAGLSQPGLDAYRGNARLVR
                     VEALLAAFPVCGQLVGAEFMRGVCRHYVSAEPAASANLCDDGARMGDFLAQFAPAAEL
                     PYLADVARFEWAWHRAAWAAAADCWRPAQLAALPPEAWADVRLSRHPAASCVASPWPL
                     GEIFACHQDNPHGDFYIDLNSGGGVWLLSRPALAPQWLALSPGQAALLEALWLNAPLG
                     DAAQAALAVEPSLNLADSLGWLAAAGAWADT"
     gene            complement(6862..7689)
                     /locus_tag="PKN55_03110"
     CDS             complement(6862..7689)
                     /locus_tag="PKN55_03110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014086156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 domain-containing protein"
                     /protein_id="HNX70266.1"
                     /translation="MLPCSAGIGLRAPHVRAALDGHPAIRWIEIHSENVFGGGPAGQL
                     ARELAARYPLSLHGVGMGLGAVEPLDAEHLRALTTLVQAVRPAAVSEHLSFNRAHGKV
                     VNDLLPLPYTEECLAHVAQRVQQVQDALGQRLLVENVSACLACPDDCIGEGEFLAELA
                     ARTGCGVLLDVNNLYVNQLNLGRDARAAMQALPARGVVAEIHLAGFIRRDGLVIDSHS
                     QPVCEAVWGLYQEALTRFGPTPTLIEWDLDIPPLDTLLAEAARAQRVLDACVVKEVA"
     gene            complement(7823..8083)
                     /locus_tag="PKN55_03115"
     CDS             complement(7823..8083)
                     /locus_tag="PKN55_03115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008953215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2282 domain-containing protein"
                     /protein_id="HNX70267.1"
                     /translation="MQASRALIASALAGLVAAGSLAAAPAMAADKEKCFGIATAGKND
                     CANALGTHSCAGQATKDKDPGDWKFVAKGNCEKMGGSLTAGK"
     gene            8082..8288
                     /locus_tag="PKN55_03120"
     CDS             8082..8288
                     /locus_tag="PKN55_03120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX70268.1"
                     /translation="MVKLLVNQLSKVANATESTAVMRRFLPLGRRSNGHPYAAYKKFC
                     PFFGRILPGLLAEQLAWLSRPIMP"
     gene            complement(8315..9358)
                     /locus_tag="PKN55_03125"
     CDS             complement(8315..9358)
                     /locus_tag="PKN55_03125"
                     /inference="COORDINATES: protein motif:HMM:NF013384.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low specificity L-threonine aldolase"
                     /protein_id="HNX70269.1"
                     /translation="MSDAPIYFTSDNAAGMCPEVLQALIAANDGPAAAYGHDPLTQAL
                     EAQFAELFEYPVTVLLVSTGTAANALSLAALTPPWGSVLCHADSHIQHDECGAPEFFT
                     AGAKLQPLAGARGKLCPDALAAALRQKKGDVHSVQPSAVSLTQATEVGSVYTLDELEA
                     IGAVCRQAGVGLHMDGARFANALARLDCSPAEMTWQRGVDVLSFGASKNGAMAAEAIV
                     VFDPGLARELAFRRKRAGHLLSKTRFCAAQLQGYLHDDAWLRHARHANAMAARLAHGL
                     AAVPGVTLNAPVEANIVFCQLPEAVLSGLQAAGFMFYTGRWAPGVARLVTSFATREDE
                     VDALLAEAGRLAG"
     gene            complement(9523..12921)
                     /gene="mfd"
                     /locus_tag="PKN55_03130"
     CDS             complement(9523..12921)
                     /gene="mfd"
                     /locus_tag="PKN55_03130"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019920750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HNX70270.1"
                     /translation="MSAALFASLRPGQKHRLGALGPGADARLLAAQIEPGLPLLVLTA
                     DAQAAQRLKEEIPVFAPAARVLLLPDWETLPYDHFSPHADLVSERLATLWQIRQGQAD
                     IILAPVSTAMTRLAPVSFLAGRTFMLSQGQKLNADALRADLALAGYTHVTQVYSPGEF
                     SVRGGLIDLFPTGSALPYRIDLFDDEIDNLRTFDVDTQRTLYPVKDVRLLPAREFPTD
                     ESGVTTFRQQFRERMEGDPSRAQIYKDVSSQCWPAGIEYYLPLFFSETATLFDYLGEH
                     ARLALHHDVLAAAEGFWQEIGSRYTLLRGDPARPLLPPGEVFIYPDALMAALKTYPRL
                     ELPADGEADASVAALPALAVDRRADDPLAALRQFVAGFAGRALIVAESLGRRETLQHF
                     LAEHGLRPQPVADFAGFVDGQTPLALLAAPLSAGFIDPAAQLAVITESELYQHSPRLR
                     RRQRSRASQDAMLKDLAEVKIGDPVVHETHGIGRYQGLVSMDLGEGESEMMMLEYADQ
                     ARLYVPVSQLHLISRWAGGAQENAPLTKLGSPQWDKAKQRAAEKARDTAAELLNLYAQ
                     RAAREGHAFALSEDDYAAFADGFAFDETPDQQAAIDAVIADMTSGKPMDRLVCGDVGF
                     GKTEVALRAAFVAVLGGKQVAVLAPTTLLTEQHFQNFADRFADWPVKIAELSRFKSGK
                     ELKAAMAGLADGSVDIVIGTHRLTQPDVEFARLGLVIIDEEHRFGVRQKEQLKRLRAN
                     VDVLTLTATPIPRTLAMSLEGLRDFSVIATAPNRRLAVKTFVTPHSHGVVREAALREL
                     KRGGQVFYLYNEVDTIENMRERLAELLPEARIGVAHGQLRERELEQVMRDFHQQRFNV
                     LLCSTIIETGIDIPNANTILIHRADKFGLAQLHQLRGRVGRSHHQAYAYLLTGEGLSR
                     DAQKRLEAIQSMEELGSGFYLAMHDLEIRGAGEVLGEGQSGEMHEVGFSLYTEMLKQA
                     VKALKKGHEPDLDAPLGVTTEINLHSPALLPEDYCPDVHERLVLYKRLASCDSDGDID
                     AIHEELIDRFGLPPQPVKTLIDCHRLRLAARELGIQKIDASEVAIQLTFIKNPPIDPM
                     KIIGLIQNKRNYKLAGQDKLRVESALPDVALRCARVKELLRELS"
     gene            13292..>14832
                     /locus_tag="PKN55_03135"
     CDS             13292..>14832
                     /locus_tag="PKN55_03135"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNX70271.1"
                     /translation="MTRHGWTALGIAAVYLAGARLPILAGDPELARALLAMPVGLLAG
                     LTARHGLRCLWATTGLTLLNAALGGFAAEAVAQSLAAWLVCAASLSTTRHLPALLDTG
                     AWRARMRVIAGFVLAAVIGALALAVSLWWLCPEFCDPDIFILQQLAVHGWSLALYVPL
                     VLAVSDWRGSVRPWELLALLLTGAVVWWLGLWDRLMASSCFFPCQFVWAPLIGWAALR
                     TGVFGVSVTGMVASALTTLSVLAHSAWPASTLVMLAYVACVLGLVQAAGLAIAALIEA
                     HRASLIALNLARNQFAALMANSASMIFIKDTAGRYLQVSHSYAALLGGEPADMVGSRL
                     ERWVDDAGTVARLTAIDAEVMLRRQPRQSEEVLVVNGHSHILLNICFPLFDERGELSG
                     MGGIAVDITAQREEERRRREAEERYQVLVEQSLVGIYIQQDEKLVYVNPKVASTLGYT
                     QEELLGRSLTELLDPKETARVRGQIGDRYQHHIENMQYTLPVRHRDGHVIEVEVYSRL
                     FEYQGR"
BASE COUNT         2574 a         4818 c         4890 g         2550 t
ORIGIN      
        1 gacaacaacc tgggctcata gctgacgtcc tggcgctgcg cgtcatgcag cttgatcttg
       61 acctgactgg gctggttgac cgatgcggtg gccatgaact ggcgcagttg cgcgctggct
      121 tgcgggtctt gcagcacccg gcgcaattta tcgctgaaca ccactttcat tgtcattctc
      181 ccaaaaccat gcatcgcggc gcaccccatg ccaatatagt atggggtgcg tcaacacacg
      241 ccagcaaacg aaaaacttac ccctcgtccc gctcgcgcag ccctggcggc agcgagaagc
      301 gcacgcgctc ttccacgccg ggcagttcgg ccacgtggtc ggcgccgagt tcgcggatgc
      361 gttcgatcac cgagcgcacc agcacgtccg gggccgaggc gccggcggtg acgcccacct
      421 gactggcctg ggcgaaccat tccggcttga gctcgctggc gttgtccacc atataggcgg
      481 tcacgccgcg cagcgcggcc acttcgcgca ggcggttgga gttggagcta ttgggcgagc
      541 cgaccacaat caccaggtca cactgggcgg tgagcgcctt gacggcgtcc tgacggtttt
      601 gcgtggcgta gcagatatcg tcttttttcg ggccgtggat gtcaggaaag cgcgcgcgca
      661 gggcgtcgat gatgtcgcgg gtttcgtcca ccgacagcgt ggtttgcgac acataggcca
      721 gcgcctgcgg gttgcgcacg ctcagcgtgg ccacgtcggc gacggtttcc accagataca
      781 tgccgtcggg cgactggccc atggtgcctt ccacctcggg gtggccggcg tggccgatca
      841 tgacgatttc ctggccggcg tcgcgcatgc ggcgcacttc cacatgcacc ttggtgacca
      901 gcgggcaggt ggcgtcgaac acgcgcaggc cgcgctgttc cgcttcggcg cgcaccgaca
      961 gcggcacgcc gtgggcggaa taaatcagcg tgctgcctgg cggcacgtcg gccaggtctt
     1021 cgataaagat cgccccttgc tcacgcagat tgttgacgac gaatttattg tgcaccactt
     1081 cgtgacgcac ataaatcggc gcgccaaact gcgccagggc gcgttcgaca atggcaatgg
     1141 cgcggtccac cccggcgcaa aagccgcgcg ggttggcaag caacacctga atgggctggc
     1201 tcatgccggt ttccttgtgg cgggttgacg aaaactgtcc agcaccatca gcgcggcgcc
     1261 caggcagatg gcgctgtcgg ccagattgaa cgccggccaa tagtaatgct gataataaac
     1321 ctggataaaa tcaatcacat ggccgtgaat cagccggtcg atgacattgc ccagcgcgcc
     1381 gccgatgatc agcgcgccgg ccaggttcat catccggctc tggccaccgc gcagcatctg
     1441 ccagcccagc cagccggaca cggcaaacgc cagcgcggtg aagaaaaact tctgccagcc
     1501 gccggcgtcg gccagaaagc tgaacgccgc gccggggtta tacaccagca caaagttgaa
     1561 caggccgggg atcacctcgc gcacttcgcc atactgatag gcgccgttga aatacacctt
     1621 gctcagttga tccagcacga tcaccagcgc cgacagcaca aaccagcgaa cggcctcagg
     1681 catgggcgcg cgcctcgccg tcgccaaaca gattgctcac gcagcggcca cacagggtgg
     1741 ggtgctcggc gtgggcgccc acgtcgtcgc gccagtgcca gcagcgctcg cacttttgcg
     1801 cttcggaggc gcgcacgctg atggcggtgt gctcgcccgc gctcagcgtc accttggaga
     1861 cgatcagcac gaatttcagg tcatcgccca gcgaggccag cagcgggtag agctcggcgt
     1921 cggcgacgat gtccacttcg gcttgcagcg acgagcccag cacattggcc acgcgctggt
     1981 cttcgatcag cttgttgacg ttggcgcgca cggcgcgcag cgcgctccag cgcgccagca
     2041 gctcggcttc gccggccact tccggcaatg cgtgccaggt gtggaacagc gcgctgtctt
     2101 cggcgtcgcc gctgaacacc gcccaggctt cgtcgctggt gaagcacagc accggttgca
     2161 gccacagcag caaggcgcgg gtgatgtgcc acagcgcggt ttgcgccgag cggcgcgccg
     2221 acgaatcggc ggcggtggtg tacaggcggt ctttgaggat gtccaggtaa aacgcgccca
     2281 ggtcgtccga gcagaaggtg acgatgtcct gcatggcgtg gtggaaggcg tagcgcgggt
     2341 acagatcgcc cgtcagccgg ctttgcagcg cgcgggtttg cgccagcgcg tagcggtcga
     2401 tttccagcag attgtccacc gccacgccgt cgcgggccag gtcgaaatcg gtcagattgg
     2461 ccagcaggaa gcggatggtg ttgcgcaggc ggcgatagct ttcggtgacg cccttcagga
     2521 tggtgtcaga aatcgccagc tcgcccgagt aatccgtcgc ggccacccac aggcgcagga
     2581 tgtcggcgcc cagggtgtcg ttgatttttt gcggggcgat gacattgccc agcgatttgg
     2641 acatcttgcg gccctggccg tccaccacaa agccgtgggt cagcaactgg tcgtacgggg
     2701 cgcggccggt ggtggcgcag ccggtgagca tcgagctctg gaaccagccg cgatgctggt
     2761 ccgagccttc cagatacaaa tccgccggcc atttcagctc gtcgcgctga cgcagcacgg
     2821 cgtagtgggt ggagccggag tcaaaccaca cgtccagggt gtcggtcagc ttgcggtatt
     2881 ggctggcctc gtcgcccagc agctcggcgg cgtccagctg gaaccacgcc tcgatgcctt
     2941 cttgttcgat gcgcagcgcc acctgttcca gcagctcggc cgaacgcggg tgcagggcgc
     3001 cggtttcttt gtgcacaaag aaggtcatcg gcacgcccca gttgcgctgg cgcgacacgc
     3061 accagtccgg gcggttgccg atcatcgctt ccagccgggc gcggccccag gccgggaaga
     3121 actgggtgtg ctccaccgcg cgcatggcgt tggcgcgcag cgccacgccg tcctggccaa
     3181 cggtgtccat gccgataaac cactggctgg tggcgcggaa gataatcggg gttttatgcc
     3241 gccagcagtg cgggtagctg tgcttgagcg cctcgctggc cagcagggcg tcgtgctcgg
     3301 tgagggtttc cagcactttc gggttggctt cccacacgct caggccggca aacagcggcg
     3361 tgccggggat aaagcggcca tcgtcgccca ccggattgtc caccgccagc tgatacttca
     3421 ggccagcctg gaagtcttcc agaccatgcg ccggggcagt atgcaccagg ccggtgccgg
     3481 cgtcggcgcc gacgtgctcg cccagaatca ccggcacctg gcgcggctgg aagggatgct
     3541 gcaagggcag caggtccagc gcggcgccct tggcctggcc cagcagttgg gcgccgtcgg
     3601 ccaagttgta gcgcttgagc gcgccttcgg ccagctcagc ggccagaatc agcgcgccgc
     3661 gcgcggtggc ccacagctgg taatccagat tcgggtgcac cgccaccgcc tggttggccg
     3721 gcagcgtcca cggcgtggtg gtccagatca ccgcgcgcgc ctggccgtcc agcgacgcca
     3781 ggccaaaggc ggcggccagg cgggcggtgt cggccaccag aaagccgacg tcaatcgccg
     3841 gcgaggtttt gtcttcgtat tccacctcgg cttcggccag cgccgagccg cagtcgatac
     3901 accagtgcac cggcttcttg ccctgcacca gatagccgtt ttggtggatt ttgcccagcg
     3961 tgcgcacgat gtccgcttcg gtgcggtagt tcatggtcag gtaggggttg tcccagtcgc
     4021 ccagcacgcc caggcggata aagtcggctt tttgccgctc gatctgcacc atggcgtaag
     4081 aacggcacag cttgcgcacc accgaggccg gcaggcgggt ttctttcggg tccaggccgt
     4141 tttgctggcg ataggcgacg atcagctgat gaatgccggg cgcggcttcg atggcttttt
     4201 tgtcgccctt gatcagtttt tcgatctggt gctcgatcgg caggccgtgg caatcccagc
     4261 ccggcacata gggcgcgtca aagccggcca gggttttcga gcggatgatg atgtctttga
     4321 gaatcttgtt gaccgcatgg ccgatgtgaa tgtcgccgtt ggcgtagggc gggccgtcgt
     4381 gcagcacaaa cttgggccgg ccggcagtgc gctgacgcag cgcctgatag cgctggccgg
     4441 cttgccactg cgccacccag ccgggttcgc gcttggccag gtcgccgcgc atgggaaagg
     4501 cggtgtccag caggttgacg gcgtatttct tggagtcggt gctcatgcgg aaactctctg
     4561 caaagtcgat agatggtggc gggcggcggc ggcgtcggca tcaatctgcg ccgtcagcgc
     4621 cgccagcgaa tcaaagcgtt gttcatcgcg cagcttggcc agaaagcgca cgcaaatcaa
     4681 ttggccgtac agattgccgg caaagtcgaa cagatggact tccagcttgt aatcgtcccc
     4741 ctgatcgacc gtggggtttt tgcccaggct ggccacgccg ccaaagcggc cggcggcggt
     4801 gtccacttcc accacaaaaa tcccggccag cgccggtttg cggtgcggca ggtgcacatt
     4861 ggcggtgggg tagcccaggg tgcggcccag ctttttgccg tgcatcaccc ggccggtgag
     4921 ctggtaatgc cggcccagca gcgcctcggc gtgggcaaag tcgcccgcgg ccagcgcctc
     4981 gcggatgcgc gagctggagg cgcgctcgcc gtgcacgctg atggtgggca tcgcctcggt
     5041 gacaaagtgc ggacaggctt gcagggtgga aaagttcccc agccggcggg cgccaaactg
     5101 gaaatcatcg ccaatcagca cgtattgcgc gcccaggcca gccaccagaa tctgatcaat
     5161 aaaatcctgc gcgctcagcg cggcaaacgc cggggtgaag cgatgcacat aaagatgatc
     5221 aagtaagccg gtggccttga gcgacagcac tttgtcgcgc agcgtggcca gccgcgccgg
     5281 cggattgccg cgggcaaaca cctcgcgcgg gtgcggctcg aaggtgagca gcgccgtggg
     5341 caagccgcgc gcgctggcgc gctcgcgcaa acaggccagc atggcctggt ggccacggtg
     5401 cacgccgtca aaattgccaa tggtcagcgc gcaggccgaa ggccgggcgg ctgaggcgcc
     5461 aaaatgaacc tgcatggctg aaaatcgctg gaaaaccttg gattctaacg ggtatcgccg
     5521 cgctgcacca caatctggcg catgcgcggc gacaacagcg gcccacgggt gcgagcgggc
     5581 catggcgggc tattttacgg atgcaaacgc cgcagcaaaa agcgatccac cgaccagccg
     5641 ccaccgccat aggccagcag cagggccagc agcatgcccc aatagacatg aaactggcgg
     5701 gcaatctctg gcatggccgg gtaggaaatc accgccatca ggttcagcgc aaacaaaccc
     5761 agcgccgcca cgcgggtggc cagccccagc gacagcagca tgggcagcag aatttcgcac
     5821 gcggtggcca gcagcgccgc cagcatcggc ggcagcacgg gcacttggta ttcgtcggtg
     5881 aacaaggcca acgtcacatc ccagtcggcc agcttggtca tgccggcgcg aaaaaacaca
     5941 tcggccaaaa ataagcgcac acccagggtc aagggcgcgc gcagggcatt ggccgcgcgc
     6001 gaccacggca ggcagcgcgc cagccaggcg cgcaaacgca gttccatcat gttttctcct
     6061 tcgcgccgtt cacaacctgc gcctggcgtc attgcaccgg gctggctcag cgattttatg
     6121 tatccgccca agcgccggcg gcagccagcc agcccaggct atcagccaga ttcaagctgg
     6181 gctccaccgc cagcgccgcc tgtgcggcgt cgcccagggg ggcattcagc cacagtgctt
     6241 ccagcagcgc ggcctgaccg ggactgagcg ccagccattg cggggcaagc gcaggtcggc
     6301 tgagcagcca cacaccgccg ccactgttca agtcaatata gaagtcgccg tgcggattat
     6361 cctgatggca agcaaaaatc tcacccagcg gccagggcga ggccacgcag ctggcggccg
     6421 ggtggcggct cagccgcaca tcggcccagg cctcgggcgg cagcgccgcc agctgcgccg
     6481 gacgccagca gtcggcagcc gctgcccagg cggcgcgatg ccaggcccat tcaaagcgcg
     6541 ccacatcggc cagatagggc agctcggcgg ccggagcaaa ctgcgccaga aaatccccca
     6601 tgcgcgcgcc gtcatcgcac agattggcgc tggccgccgg ctcggcgctg acatagtgac
     6661 ggcacacacc gcgcatgaat tctgcgccaa ccagctgccc gcacaccgga aacgccgcca
     6721 gcagcgcctc cacccgcacc agccgggcat tgccgcgata ggcgtccagc cccggctggc
     6781 tcagcccggc cggcaagggc gacggcgctt caggcgcggt gatggcgtca atcagctggc
     6841 tttgccagcg cagcagcgcg ctcatgccac ctccttgacc acacaggcgt ccagcacgcg
     6901 ctgggcgcgg gcggcttcgg ccagcagggt atccagcggg gggatgtcca aatcccattc
     6961 aatcagcgtc ggcgtcggac caaagcgcgt cagcgcctct tggtacagac cccataccgc
     7021 ttcgcacacc ggctggctgt gcgaatcaat caccaggccg tcgcggcgga taaacccggc
     7081 caggtggatt tccgccacca cgccgcgcgc gggcagcgcc tgcatggcgg cgcgggcgtc
     7141 gcggcccagg ttcagttggt tgacgtacag attattgacg tccagcagca cgccgcagcc
     7201 agtgcgggcg gccagctcgg ccagaaactc gccctcgcca atacaatcat ccgggcaggc
     7261 cagacaggcc gagacattct ccaccagcag gcgctggccc agcgcatcct gcacctgctg
     7321 cacgcgctgg gcgacgtgcg ccaggcattc ttcggtgtag ggcagcggca agagatcatt
     7381 caccaccttg ccgtgcgcgc ggttgaacga cagatgctcg gacaccgccg ccggccgcac
     7441 cgcctgcacc agcgtggtca gcgcgcgcag gtgctcggcg tccagcggct cgaccgcgcc
     7501 cagacccatg cccacgccgt gcaggctgag cggatagcgc gcggccagct cacgcgccag
     7561 ttggccggcc gggccgccac caaacacgtt ttcgctgtgg atttcaatcc agcggatggc
     7621 cgggtggccg tccagcgcgg cgcgcacatg cggcgcgcgc aggccgatgc cggcggaaca
     7681 aggcagcatg gaaaactcca gaggcaggaa attaaagaga aaaatctggc gggagccgca
     7741 gggcaaaaca tcgccgccga cgctttgcct tgccgcccca ggcccggcgc aactggctgc
     7801 cgggcccaaa gcaaagcggt gcttacttgc ctgcggtcag cgagccgccc attttttcgc
     7861 agttgccctt ggcgacaaat ttccagtcac ccggatcttt gtccttggtg gcctgaccgg
     7921 cgcagctgtg ggtgcccagg gcgttggcgc agtcgttttt gccggcggtg gcgatgccga
     7981 agcatttttc tttgtcggcg gccatggccg gggcggcggc cagcgagcca gcggccacca
     8041 ggccagccag ggcggaagcg atcagagcgc gggatgcttg catggtgaaa ctccttgtca
     8101 atcaactgag taaagtggcc aatgccaccg aatcaacggc ggtgatgcgc cgttttctgc
     8161 cccttggtcg gcgcagcaac ggacatcctt acgcagcata caaaaaattt tgcccatttt
     8221 ttggaaggat tttgcctggc ctgcttgccg aacagttggc atggctatct cgaccaatca
     8281 tgccttgacg ccagccgggt ttggcaagcc ccgcttagcc tgccagtcgg cccgcttccg
     8341 ccaacaacgc atccacctca tcctcgcggg tggcgaacga agtcaccagc cgcgccacgc
     8401 ctggcgccca gcggccggtg taaaacataa agcccgccgc ctgcaagccg ctcagcaccg
     8461 cctcgggcag ttggcaaaac acgatattgg cctcgaccgg cgcgttcagc gtcactcccg
     8521 gcacggcagc caggccgtgc gccaggcgcg cggccatggc gttggcgtgg cgggcgtgac
     8581 gcagccaggc gtcgtcgtgc agatagcctt gcagctgggc ggcgcaaaag cgggtttttg
     8641 acagcaaatg gccggcgcgc ttgcggcgga aggccagctc gcgcgccagg ccaggatcga
     8701 acaccacaat cgcctcggcc gccatcgcgc cgttcttgct ggcgccaaac gacagcacat
     8761 ccaccccgcg ctgccaggtc atttctgctg gcgagcaatc cagccgcgcc agcgcattgg
     8821 cgaaccgcgc gccatccata tgcagcccca ccccggcctg ccggcacacc gcgccaatcg
     8881 cctccagctc gtccagcgtg taaacactgc ccacctcggt ggcctgggtc aggctgacgg
     8941 cggatggctg cacgctgtgc acatcgcctt ttttctgccg caaggccgcc gccagcgcat
     9001 ccgggcagag cttgccccgc gcgccagcca gcggctgcaa tttggccccg gcggtaaaaa
     9061 actccggcgc gccgcattca tcatgctgga tatggctgtc ggcatgacac agcacactgc
     9121 cccacggcgg cgtcaacgcc gccaggctca gcgcattggc ggcggtgccg gtgctcacca
     9181 gcaataccgt caccggatac tcgaacagct cggcaaactg cgcctccagc gcctgggtca
     9241 aggggtcatg accatacgcc gccgccggcc catcgttggc ggcaatcagc gcctgcaaca
     9301 cctccgggca catgccggcg gcgttgtcgc tggtgaagta aatcggggca tcagacattc
     9361 agggctttct gtgtgggtca ggtcgatggg gaaaatccag catatattta gcgtcgctca
     9421 caaaacccag cgcagcggtc ctggcgaggc gtgtgaccgc agacagtaca agtcgtacgg
     9481 caaggtcaca caacaacgcc aggagggttt tgtgcgcggc gctcaactca actcccgcag
     9541 caactccttc acccgcgcac agcgcagcgc cacgtccggc agggcgcttt ccacccgcag
     9601 tttgtcttgc ccggccagtt tgtaattgcg cttgttctgg atcagcccga tgattttcat
     9661 cgggtcaatc ggcgggtttt tgataaaggt gagttgaata gccacttcgc tggcgtcgat
     9721 tttctggatg cccagttcgc gggcggccag gcgcaggcgg tggcagtcga tcagggtttt
     9781 gaccggttgc ggcggcaggc cgaagcggtc gatcagttct tcgtggatgg cgtcgatgtc
     9841 gccgtcgctg tcgcagctgg ccaggcgctt gtacagcacc agccgctcgt gcacgtccgg
     9901 gcagtagtct tccggcagca gcgccgggct gtgcagattg atttcggtgg tgacgcccag
     9961 cggggcgtcc aggtcgggtt cgtggccttt tttcagcgcc ttgaccgcct gtttgagcat
    10021 ttcggtgtac agcgagaagc ccacttcgtg catttcgccg gattggccct cgcccagcac
    10081 ttcgccggcg ccacggattt ccaggtcgtg catggccaga taaaagccgc tgccgagttc
    10141 ttccatcgac tgaatcgctt ccagccgttt ttgcgcatcg cggctcagcc cttcgccggt
    10201 cagcaggtag gcgtaggcct ggtggtggct gcggccgacg cggccgcgca gctggtgcag
    10261 ctgggccagg ccaaatttgt cggcgcggtg gatcaggatg gtgttggcgt tggggatgtc
    10321 gatgccggtt tcgatgatgg tcgagcacag cagcacattg aagcgctgct ggtgaaaatc
    10381 gcgcatcacc tgctccagct cgcgttcgcg cagttggccg tgcgccacgc cgatgcgcgc
    10441 ttctggcaat agctcggcca gccgttcgcg catgttttcg atggtgtcca cttcgttata
    10501 cagataaaac acctggccgc cgcgcttgag ctcgcgcagc gccgcctcgc gcaccacgcc
    10561 atggctgtgc ggggtgacaa aggttttcac cgccagccgg cggttggggg cggtggcgat
    10621 caccgagaag tcgcgcaggc cttccaggct catcgccagg gtgcgcggaa tcggcgtggc
    10681 ggtcagcgtc agcacgtcca cattggcgcg caggcgcttg agctgttctt tctggcgcac
    10741 gccaaagcgg tgttcctcgt cgatgatcac caggcccagc cgggcaaatt ccacatcggg
    10801 ctgggtcagc cggtgggtgc cgatcacaat gtccacgctg ccgtcggcca ggccggccat
    10861 ggcggccttg agctctttgc cgctcttgaa gcgcgacagc tcggcgattt tcaccggcca
    10921 gtcggcaaag cggtcggcga aattctgaaa gtgctgttcg gtcagcaggg tggtcggcgc
    10981 cagcaccgcc acctgtttgc cgcccagcac cgccacaaac gccgcgcgca gggccacttc
    11041 ggttttgcca aagcccacat cgccgcacac cagccggtcc atcggcttgc ccgaggtcat
    11101 gtcggcaatc accgcgtcga tggcggcttg ctggtcgggg gtttcgtcaa aggcaaagcc
    11161 gtcggcaaag gcggcgtagt cgtcttcgga caaggcaaag gcgtggcctt cgcgggcggc
    11221 gcgctgggcg tacaggttca gcagctcggc ggcggtgtcg cgggcttttt cggcggcgcg
    11281 ctgtttggcc ttgtcccatt gcggcgagcc cagtttggtc agcggggcgt tttcctgcgc
    11341 gccgccggcc cagcgcgaaa tcagatgcag ctggctcacc ggcacataca gccgcgcctg
    11401 gtcggcgtac tccagcatca tcatctcgga ttcgccctcg cccaggtcca tcgacaccag
    11461 gccctgatag cggccgatgc cgtgggtttc gtgcaccacc gggtcgccaa tcttgacctc
    11521 ggccaggtct ttgagcatgg cgtcctggct ggcgcggctg cgctggcggc ggcgcaggcg
    11581 cgggctgtgc tggtagagct cgctttcggt gatcaccgcc agttgggcgg ccgggtcgat
    11641 aaagccggcg ctcagcggcg cggccagcag cgccagcggc gtctgcccgt caacaaagcc
    11701 ggcaaagtca gccaccggct gcgggcgcag gccgtgctcg gccagaaaat gttgcagggt
    11761 ttcccgccgg cccaggcttt ccgccacgat cagcgcgcgg ccggcaaagc cggcgacaaa
    11821 ctgacgcagg gcggccagcg ggtcgtcggc gcggcggtcc accgccagcg ccggcagcgc
    11881 cgccacgctg gcgtctgctt cgccgtcggc gggcagctcc aggcgcgggt aggttttcag
    11941 cgccgccatc agcgcgtccg ggtagataaa cacctcgcca ggcggcagca gcggccgcgc
    12001 cgggtcgccg cgcaataggg tgtagcgcga gccgatttct tgccagaagc cttccgccgc
    12061 cgccagcacg tcgtgatgca gcgccagccg ggcgtgttcg cccaggtagt cgaacagggt
    12121 ggcggtttcg ctgaaaaaca gcggcagata gtattcgatg ccggccggcc agcactggct
    12181 ggacacgtct ttgtagattt gcgcgcgcga cgggtcgcct tccatgcgct cacgaaactg
    12241 ctggcggaag gtggtgacgc cgctttcatc ggtggggaat tcccgcgccg gcagcagacg
    12301 cacgtctttc accgggtaga gcgtgcgctg ggtgtccacg tcgaaggtgc gcagattgtc
    12361 gatttcgtcg tcgaacagat caatgcggta gggcagcgcc gagccggtgg gaaacagatc
    12421 aatcaagccg ccgcgcaccg agaattcgcc ggggctgtac acctgggtga catgggtgta
    12481 gccggccagc gccaggtcgg cgcgcagggc gtcggcgttg agcttttgcc cctggctgag
    12541 cataaaggtg cgcccggcca gaaagctcac tggcgccagc cgggtcatgg ccgtgctgac
    12601 cggggccagg atgatgtcgg cctgaccctg gcggatttgc catagcgtgg ccagccgctc
    12661 ggacaccagg tcggcgtgcg gcgaaaaatg gtcgtagggc agggtttccc agtcgggcag
    12721 caacagcacg cgcgcggcgg gggcaaacac cgggatttct tctttcagcc gttgcgccgc
    12781 ctgggcgtcg gcggtcaaca ccagcagcgg caggccgggt tcgatctggg cggccagcag
    12841 gcgggcgtcg gcgccggggc cgagcgcgcc cagacggtgt ttctggccgg gacgaagaga
    12901 ggcaaacaag gcagcagaca tgggctgggc cacaacaaca agaaagacgc gattgtagcc
    12961 cagccaggca atgtgcttcg agtgccagac atcggctcac cttgatccaa gtcatgccat
    13021 acgtataaat cacgggtgtt tgtctcagct tgttcataca atcggcgcat atcggtatga
    13081 ttggatgccc tggcgcgcaa acctgccacc aggacaacac atgcaacatc atcgcgccac
    13141 gcccgcacat ccgtagggca ggctgccaat aaacaccgct caccccctat ccgccaggat
    13201 ggcgccagca gcctagtctg tcgctgcggc ccaggatggc gatgcagtag ggcgtgaagg
    13261 gtgccaagaa cacagtaagg ttgacatcat cgtgacgcgc catggctgga ctgctttagg
    13321 aatcgccgcc gtatacctgg ccggcgcgcg cctgcccatt ctggcgggcg acccggaact
    13381 cgcccgcgcc ctgctggcca tgccggtggg cctgctggcc ggcttgactg cccgccatgg
    13441 cctgcgctgc ttgtgggcca ccaccgggct gaccctgttg aatgccgcgc tgggtggctt
    13501 tgccgccgag gcggtggcgc aatcgctggc cgcctggctg gtgtgcgcgg ccagcctgtc
    13561 caccacccgc catctgcccg ccctgctgga taccggcgcc tggcgcgcgc gcatgcgcgt
    13621 gattgccggc tttgtgctgg cggccgtcat tggtgcgctg gcgctggcgg tcagcctgtg
    13681 gtggctatgc ccggaattct gcgacccgga tattttcatc ctgcaacagc tggccgtgca
    13741 tggctggtcg ctggccttgt atgtgccgct ggtgctggcg gtgtcggact ggcgcggcag
    13801 cgtgcggccg tgggaactgc tggcactgct gctgactggc gcagtggtct ggtggctggg
    13861 cctgtgggac cggctgatgg cttcgtcgtg cttttttccc tgccagtttg tctgggcgcc
    13921 gctgattggc tgggccgcct tgcgcaccgg ggtgtttggc gtatccgtaa ccggcatggt
    13981 ggccagcgcc ctgaccacgc tgtcagtgct tgcccactcg gcctggccgg cctccaccct
    14041 ggtcatgctg gcctatgtgg cctgcgtgct ggggctggtg caggcggcgg ggctggcgat
    14101 tgccgccctg atcgaagccc atcgcgccag cctgattgcg ctgaatctgg cgcgcaacca
    14161 gtttgctgcg ctgatggcca acagcgcctc gatgattttc atcaaggata ccgccggacg
    14221 ctatttgcag gtcagccaca gctatgccgc cttgctgggc ggcgagcccg ccgacatggt
    14281 gggctcgcgc ctggaacgct gggtagacga tgccggcacg gtggcacggc tgacggccat
    14341 cgatgccgaa gtgatgctgc gccgccagcc caggcaaagc gaagaagtgc tggtggtgaa
    14401 cggacactcc catatcctgc tgaacatctg ctttccgctg tttgacgagc gtggcgagct
    14461 gtccggcatg ggtggcattg ccgtggacat caccgcccag cgcgaggaag agcgccgccg
    14521 ccgcgaggca gaagaacgct atcaggtgct ggtggagcaa tccctggtgg gtatctacat
    14581 ccagcaggat gaaaaactgg tctacgtcaa tcccaaggta gccagcacgc tgggctatac
    14641 ccaggaagaa ctgctcggac gttcgctgac cgagctgctg gaccccaaag aaaccgcacg
    14701 ggtgcgcggc cagatcggcg accgctatca gcatcatatc gaaaacatgc aatacacctt
    14761 gccggtgcgc caccgcgatg gccatgtgat cgaggtggaa gtctacagcc ggctgtttga
    14821 ataccagggc cg
//
LOCUS       DAOPYX010000035        14202 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_4657_length_14202_cov_7.864494, whole genome shotgun sequence.
ACCESSION   DAOPYX010000035 DAOPYX010000000
VERSION     DAOPYX010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 14202)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14202)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14202
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4657_length_14202_cov_7.864494"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            235..2220
                     /gene="mnmC"
                     /locus_tag="PKN55_03140"
     CDS             235..2220
                     /gene="mnmC"
                     /locus_tag="PKN55_03140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011135493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)-methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016645 - oxidoreductase activity, acting
                     on the CH-NH group of donors [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HNX70272.1"
                     /translation="MTASSPLLDWQDGQPVSTRFGDVYFSRDSGLDETRHVFLAHNRL
                     AERWAALPADGRFVLGETGFGTGLNVLCAWQLWRRCAPAGARLHVISTEKYPIPPEQL
                     ARALALWPELAEFSQALLAQYAALGPGWHRFSFDEGAVTLTLLVGDALDTLPQLDAAS
                     SAVDAWFLDGFAPARNPELWSPALFAQLARLSAPGASYATFTSAGVVKRGLAEAGFSV
                     RRVAGFGSKRHMLCGELAAPPAPAWRAPWFAWPRHTGQRRALVLGGGIAGAAAAAALA
                     RRGWAVTLLEQKAALADGASGNAQGVLHTKLSPHLTPLTRLLLSGYAYSLRQLPRQLR
                     AGEDWDACGIVQQSFDADEAQRHAGLAALGLPEALLRPVDAAQASTLAGVALPHGGLW
                     FAQGGWVHPPAWVRQLAATPGVEVCCGADIVALEHDGEQWQARAADGRQWQAPVVVVA
                     NAHQAGQFSQLAHLPIKPIRGQVSHAPATEASLALRAVVCGESYISPARHGRHCFGAT
                     FRFQAEQLAVTVEEHQENLDKLAALSPDGWRRLGGEALSAQALDGRSGWRATTPDYLP
                     LVGPVADAAAFADAYPAPERCGPRQPGPDAPWLPGLYVSLAHGSRGLVTAPLAGELLA
                     AMLNHEPAPLPQPLLDALHPNRFLWRAQKRRHAPERA"
     gene            2220..2597
                     /locus_tag="PKN55_03145"
     CDS             2220..2597
                     /locus_tag="PKN55_03145"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNX70273.1"
                     /translation="MTVSHYADCPPYRTKDGSEIRELMHPDVHGNHGQSLAEARVPAG
                     GRTLAHRHARSEELYHVTAGAAWLYRDGQKVWLQAGDTARIAPGCVHWLDNPGPAELA
                     VLCCCAPAYAHTDTELIDEASDV"
     gene            2590..3294
                     /locus_tag="PKN55_03150"
     CDS             2590..3294
                     /locus_tag="PKN55_03150"
                     /inference="COORDINATES: protein motif:HMM:TIGR01621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01621 family pseudouridine synthase"
                     /protein_id="HNX70274.1"
                     /translation="MFEVLAVTERWVLIDKHPDVSFHREDADIGLLDALRAATGLPAL
                     YPVHRLDAITSGLLLFARDPATAAALGRAWQAGEVGKLYLAIAPGKPSKKQGWVKGDM
                     EKSRRGGWKLLHSRANPAVTWFDSVSLAPGLRLYLLRPYTGRTHQLRVALKSLGAPIL
                     GDALYGPAQHDSDRGYLHAALLSFALDGQRWTYTCLPSRGQWFAQPDSQTQMAALLAA
                     GRARLEEEMPTGSTLD"
     gene            complement(3515..3787)
                     /locus_tag="PKN55_03155"
     CDS             complement(3515..3787)
                     /locus_tag="PKN55_03155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX70275.1"
                     /translation="MKAALIWNSPALPGRQLRITRPHLYWLAALMAGALAATLWIALT
                     ALEFVQQGNGALDTHMAAGGLLLLALGALAACVACLLLLYELRPQE"
     gene            complement(4067..5077)
                     /gene="holA"
                     /locus_tag="PKN55_03160"
     CDS             complement(4067..5077)
                     /gene="holA"
                     /locus_tag="PKN55_03160"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011134062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HNX70276.1"
                     /translation="MPAKSLEQLPRELAAGLGPLYVIHGEEMLLALEAADSIRAAARA
                     QGYDDREVLTVESQHFDWSQLVEASLSVSLFASRKLLEIRLPGGKPGAAGSEALIHYA
                     ARLPADTVTLILLPKLDKTQLNSKWFEALSAAGVTLAAQPVDRQHLPNWVAGRLARQQ
                     QRLNDEAMQFFVERVEGNLLAARQEVDKLALLFPAGATLTLADLQSAVANVARYDVFQ
                     LSEAWLSGDAPRTARMLDGLQAEGETPVLVVWRLADELRGLVRIRQGLREGRPMAQLL
                     RDNRVWGERQRHIEHALRRLSLRRLADALAACAEVDQAIKGAASDDPWLLLRRLALGL
                     AS"
     gene            complement(5058..5585)
                     /gene="lptE"
                     /locus_tag="PKN55_03165"
     CDS             complement(5058..5585)
                     /gene="lptE"
                     /locus_tag="PKN55_03165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014088692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HNX70277.1"
                     /translation="MNRRTWLGAAAAALLLSACGFHLRGVGGEVRPLPFSSVWLATGA
                     TQLHPHLERQLRFRPDVQMVSQPQAAEAILTVTDERTAKDILTINRGGKVNEYELTYR
                     VTVRVTKNGVGYGEPITVLTRREFSYTDSQVLGKEQEEQLLFRDMREDAATQIVRRLA
                     ALKPVVELPDAGKKP"
     assembly_gap    5725..5824
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(6021..8639)
                     /gene="leuS"
                     /locus_tag="PKN55_03170"
     CDS             complement(6021..8639)
                     /gene="leuS"
                     /locus_tag="PKN55_03170"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HNX70278.1"
                     /translation="MQEHYSPRDIEPAVQARWEKNQIFKAVEDTSRPKFYCLSMFPYP
                     SGKLHMGHVRNYTIGDVLSRYKALNGFNVLQPMGWDAFGMPAENAAMKHNVAPAAWTY
                     ENIAYMKQQLKSLGFAIDWDREVTTCKPDYYRWEQWLFTRLFQKGLIYKKNGVVNWDP
                     VDQTVLANEQVIDGRGWRSGALVEKRDIPMYYFRITAYADELLADLDTLDWPPQVVTM
                     QKNWIGKSFGADITFAYDQASIGHAGQLKVYTTRPDTLMGATYVAVAAEHPLATQAAQ
                     GNAELAAFIAECKSGSVAEADMATMEKKGMNTGLFVIHPLTGEKLPVWVANYVLMGYG
                     EGAVMAVPAHDERDYAFANKYQLPMVQVLKPTADGLPEFDATVWHDWYAMKEDGATVT
                     VNCGKYDGLSVSAAFDAIVADLAAKQAGEKKTQYRLRDWGISRQRYWGCPIPIIHCDS
                     CGDVPVPDDQLPVVLPEDVVPDGMGSPLAKMPEFYQTTCPCCGKPARRETDTMDTFVE
                     SSWYFARYASPDCTTGMVDERANYWMNVDQYVGGIEHAILHLLYARFFNKLMRDEGLL
                     NNTEPFQRLLTQGMVIADTFYRDLPDGKKDWINPADVDMQRDGKGKIIQAVLRADGQP
                     VVIGGTEKMSKSKNNGVDPQALIEQYGADTARLFMMFAAPPSQSLEWSDAGVEGAFRF
                     LKRLWKTVHEHVAAGVASAYQGGELTAAEKALRFKLHSTIQKVSDDYGVRQQFNTAIA
                     AVMELLNTLDKTALSAEQGRAVAQETLESAVLLLAPIVPHVTDALWSELRPGTTLEAQ
                     RWPAVDASALVQDEIELMVQVNGKLRGSITVAKDADRAVIEAAALANENVQKFTEGQP
                     PKKVVVVPGRLVNIVV"
     gene            complement(8825..9649)
                     /locus_tag="PKN55_03175"
     CDS             complement(8825..9649)
                     /locus_tag="PKN55_03175"
                     /inference="COORDINATES: protein motif:HMM:NF016629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HNX70279.1"
                     /translation="MYILLECQSRSGPHMPVRMLQYVAALYDHLLRSDTMDIRDGLPP
                     VLPIVLYNGDARWTHPAELAPLIQPHPPALAPFQPSLRFWLLDQGVWLDARWRGVMAA
                     IFRFEHTRDVDSAKRAIRELAEQIAHSPARRVLDRVLMRWVQHRLNRKLPGIDAIQHH
                     HLIESMDMLETNIDRWKEQALAEGREQGMLLGMQQGMQQGIQQGMASGIEQGRQQGLA
                     QGERQLLQRLLDRRFGPLPDWALARLAHADAAQLEAWSEAMLDAAALSEVLGTPPH"
     gene            complement(9799..9984)
                     /locus_tag="PKN55_03180"
     CDS             complement(9799..9984)
                     /locus_tag="PKN55_03180"
                     /inference="COORDINATES: protein motif:HMM:NF016629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HNX70280.1"
                     /translation="MSDAHHDSGYKYLFSHAELVRELLETFAPPGVAAQLDYATLRRE
                     NGHYVTPALKQREQDVV"
     gene            complement(10166..10732)
                     /locus_tag="PKN55_03185"
     CDS             complement(10166..10732)
                     /locus_tag="PKN55_03185"
                     /inference="COORDINATES: protein motif:HMM:NF016819.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HupE/UreJ family protein"
                     /protein_id="HNX70281.1"
                     /translation="MRKSLLILPLALASSAALAHSGHAGHDSAMLAGLSHPLGGADHL
                     LAMLAVGLWAGQQGGAARWQAPLAFVALLAVGGALGAAGLWLPGVEAGIAASVLALGL
                     LLAARARLPRWAGLGLTGGFALLHGLAHGAEMPASASLLAYGAGFMAASAALHLAGLA
                     LYLPLHRISRAIGALLAVAGVGLLLGMA"
     gene            complement(10725..11357)
                     /gene="ureG"
                     /locus_tag="PKN55_03190"
     CDS             complement(10725..11357)
                     /gene="ureG"
                     /locus_tag="PKN55_03190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014087182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreG"
                     /protein_id="HNX70282.1"
                     /translation="MSATPLRVGIGGPVGSGKTHLTWALCAALRERYNIAVVTNDIYT
                     QEDAQFLVRNEALSPDRIIGVETGGCPHTAIREDASINLEAVARLNARHPGLDVIFVE
                     SGGDNLAATFSPELSDLTIYVIDVSAGDKIPRKGGPGICKSDLLVINKIDLAPLVGAS
                     LEVMARDAKTMRGERPFLFSNLKTGQGLADIIALIEEKGMLNLDPEASHA"
     gene            complement(11508..12188)
                     /locus_tag="PKN55_03195"
     CDS             complement(11508..12188)
                     /locus_tag="PKN55_03195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008954173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory UreF family protein"
                     /protein_id="HNX70283.1"
                     /translation="MTPLAALQCLRLASPGLPIGAYSYSQGLETAIDLGQIHDRASAE
                     HWLRGLLAGPVSRYDAPMLARAHRASAANDAPALVALNQTLLASRDSREQRQESVQMG
                     YSLRQLLAALPETASQPLPAALQDGELAFLLAYGWAGCRLGLDAATVVQAYLWGWLEN
                     QVMVLMKALPMGQTAGQQLMSALLPALAAASEQALTLPDARLSAFAPMQSWMALRHET
                     QYSRLFRS"
     gene            complement(12185..12703)
                     /gene="ureE"
                     /locus_tag="PKN55_03200"
     CDS             complement(12185..12703)
                     /gene="ureE"
                     /locus_tag="PKN55_03200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014087180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreE"
                     /protein_id="HNX70284.1"
                     /translation="MLTLTRHAEPGEHWDAELAMTHELRCKTRLRTRSADGEDVALFL
                     AAGSPPLADGDRLLANDGRVARVRALPEPLLHVTAADARELTRAAYHLGNRHVPVQVG
                     EGWLRLAEDSVLHAMLDQLGVSVQALDAPFQPESGAYGGGHHHSHGKDSVFQYAPRLH
                     QFRRDGSPDDSA"
     gene            complement(12712..>14202)
                     /gene="ureC"
                     /locus_tag="PKN55_03205"
     CDS             complement(12712..>14202)
                     /gene="ureC"
                     /locus_tag="PKN55_03205"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014087179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit alpha"
                     /protein_id="HNX70285.1"
                     /translation="NALILDHWGIVKADIGLKAGRIAGIGKAGNPDIQPGVTLLIGAS
                     TEIIAGEGCIITAGGIDTHIHFICPQQIEEALMSGVTTMIGGGTGPATGTNATTCTSG
                     PWHLAQMLKAADSFPMNLGFTGKGNASLPEPLREQVRAGAIGLKLHEDWGTTPAAIDN
                     CLSVADEMDVQVAIHTDTLNESGFVEATFAAFKGRVIHTYHTEGAGGGHAPDIIKACG
                     QPNVLPSSTNPTRPYTVNTIDEHLDMLMVCHHLDPSIAEDVAFAESRIRRETIAAEDI
                     LHDLGAFSMISSDSQAMGRVGEVILRCWQTADKMKRQRGALPDDGPGNDNFRAKRYIA
                     KYTINPALTHGVAHEVGSVEVGKLADLVLWKPAFFGVKPSLILKGGMIAAAAMGDANA
                     SIPTPQPVHYRPMFGAFGAAMTATSVTFVSQAALDANIGQQLGLAKRLVAVQGCRSVQ
                     KTDLIHNNWLPDISVDPQTYQVRANGELLWCEPAEVLPMAQRYFLF"
BASE COUNT         2237 a         4599 c         4798 g         2468 t
ORIGIN      
        1 gcgcacggcc cgtgcaatat ttgcgtagcg ccgccaaccc tcctgacgtt gttgtgcggc
       61 ctggccagta ctgcctgtac tgtctgcggc cgcacacctc atcaggaccg ctgcacgggg
      121 ttgcgcgcgc gacgcttaaa ccatgataaa gccaggtcgc gccacaccca ccctgcccgc
      181 cagcggctgg ctgggctatc atccggcttt atatttgctg atgtttgacc gcgcatgact
      241 gcttcaagcc ccctgctgga ttggcaggat ggccagccgg tgtccacccg gtttggcgat
      301 gtgtatttct cccgcgatag cggcctggat gaaacccgcc atgtgtttct ggcgcataac
      361 cgcctggccg agcgctgggc ggccctgcct gcggatggcc gctttgtgct gggcgagacc
      421 ggctttggca ccgggctgaa tgtcttgtgc gcctggcagc tgtggcggcg ctgtgcgcca
      481 gcgggcgcgc ggctgcatgt gatcagcacg gaaaaatacc ccatcccccc cgagcagctg
      541 gcgcgcgcgc tggccttgtg gcccgagctg gccgagttca gccaggcgct gctggcgcaa
      601 tatgccgcgc tggggccggg ctggcatcgc ttcagttttg atgagggcgc ggtcacgctc
      661 accttgctgg tgggcgacgc gctggacacc ctgccgcagc tggacgccgc cagcagcgcg
      721 gtggacgcct ggtttctgga tggctttgcc ccggcgcgca acccggagct ttggtcgccc
      781 gcgctgtttg cccagctggc gcggctgtcg gcgccggggg ccagttacgc cacatttact
      841 tctgccggcg tggtcaagcg cggcctggcc gaggcgggct ttagcgtgcg gcgggtggct
      901 ggctttggca gcaagcggca tatgctgtgc ggcgagctgg ccgcgccgcc cgcgccggcc
      961 tggcgcgcgc cgtggtttgc ctggccgcgc cacaccggcc agcgacgcgc gctggtgctg
     1021 ggcggcggca ttgccggcgc ggcggcggcg gcggcgctgg cgcggcgcgg ctgggcggta
     1081 accctgctgg agcaaaaagc ggcgctggcc gacggcgctt ctggcaatgc ccagggcgtg
     1141 ctgcacacca aattgtcgcc gcacctcacc ccgctcaccc gtttgttatt gtccggctac
     1201 gcctacagcc tgcgccagct gccccggcag ctgcgcgctg gcgaggattg ggacgcctgc
     1261 ggcattgttc agcagagttt tgacgcagac gaagcccagc ggcacgccgg cctggcggcg
     1321 ctgggcctgc ccgaggcgct gctgcgcccg gtggatgcgg cgcaggccag cacgctggcc
     1381 ggggtggcct tgccgcatgg cgggctgtgg tttgctcagg gcggctgggt gcaccctccg
     1441 gcctgggtgc gccagctggc ggccacgccg ggggtggaag tgtgttgcgg ggcggacatc
     1501 gtcgcgctgg agcacgacgg cgaacaatgg caggcgcgcg cggcggacgg tcgccagtgg
     1561 caagcgccgg tggtggtggt ggccaatgcc catcaggccg gccagttcag ccagctggcg
     1621 catttgccga tcaagccgat tcgcggccag gtcagccatg cgccggccac cgaggccagc
     1681 ctggcgctgc gcgcggtggt gtgcggcgag tcgtatatct cgccggcccg ccatggccgc
     1741 cactgctttg gcgcgacttt tcgttttcag gccgagcaac tggccgtcac cgtcgaagag
     1801 catcaagaaa acctggataa actggccgcg ctcagcccgg acggctggcg gcgattgggg
     1861 ggcgaggcgc tcagcgcgca ggcgttggac ggccgttcgg gctggcgcgc caccacgccg
     1921 gactatctgc ccttggtcgg cccggtggcc gacgctgccg cctttgccga cgcctacccg
     1981 gcccccgagc gctgcggccc gcgtcagccg gggccagacg cgccgtggct gccggggctg
     2041 tatgtttcgc tggcgcatgg ctcgcgcggc ttggtgacgg cgccgctggc tggcgagctg
     2101 ctggcggcca tgctcaacca cgaaccggcg ccgctgccgc agccgctgct ggatgccctg
     2161 catcccaacc gttttttatg gcgcgcgcaa aagcgccgcc acgccccgga gcgcgcctga
     2221 tgactgtctc tcactacgcc gactgcccgc cctaccgcac caaggatggc tcggagatcc
     2281 gcgagctgat gcacccggac gtgcacggca accacggaca aagcctggcc gaggcgcgcg
     2341 tgccggccgg cgggcgcacg ctggcgcacc gccacgcgcg cagcgaagag ctctaccacg
     2401 tcactgccgg cgccgcctgg ctgtaccgcg atgggcaaaa agtgtggctg caagccggcg
     2461 acaccgcgcg cattgccccc ggctgcgtgc actggctgga caaccccggc ccggccgagc
     2521 tggccgtgct gtgctgctgc gcgccggcct acgcccacac cgacaccgaa ctgatcgacg
     2581 aggccagcga tgtttgaggt gctggccgtc accgagcgct gggtgttgat cgacaaacac
     2641 ccggacgtgt cgtttcaccg cgaagacgcc gatatcggcc tgctggacgc cctgcgcgcc
     2701 gccaccggcc tgccggcgct gtacccggtg caccggctgg acgccatcac ctccggcctg
     2761 ctgctgttcg cccgcgaccc ggccaccgcc gccgcgctgg gccgcgcctg gcaggccggc
     2821 gaggtgggca agctgtatct ggccattgcg ccgggcaagc cgagcaaaaa gcaaggctgg
     2881 gtgaagggcg atatggaaaa atcccgccgc ggcggctgga agctgctgca cagccgcgcc
     2941 aacccggccg tcacctggtt tgacagcgtc agcctggccc ccggcctgcg cctttacctc
     3001 ttgcgcccct acaccggccg aacccaccag ctgcgagtgg cgctgaaaag cctgggcgcg
     3061 ccgattctgg gcgatgcgct ctatggcccg gcccagcacg acagcgaccg tggctatctg
     3121 cacgccgcgc tgctgagctt tgccctggac ggccagcgct ggacctacac ctgcctgccc
     3181 agtcgcggcc aatggtttgc ccagccagac agccagacgc agatggcggc gctgctggca
     3241 gcgggtcggg caaggctgga ggaagaaatg ccgacggggt cgacgttgga ttaagagcgg
     3301 ctcacaaaac catcctggcc ttgttgcccg accttgccgt actttgctgt actgtctgcg
     3361 gtcgtgcgcc tagccaggat cgctgcgctg ggttttgtga gccgctctga cggcggcccc
     3421 gtgcgagtat ggcggagaga tagggattcg aactcagccg cagcccaaga gcggcgcacc
     3481 ctgcattcgg ttttgtgggc gtagtggggt tcggctactc ctgcgggcgc agctcataca
     3541 gcagcagcaa gcacgccaca cacgccgcca gcgcgcccag cgccagcaag agcaggccgc
     3601 ctgcagccat gtgtgtatcc agcgcgccat tgccttgctg gacaaattcc agcgcggtca
     3661 gcgcaatcca caaggtggcg gccagggcgc cggccatcag cgcggccagc cagtacagat
     3721 ggggacgggt gatgcgcagt tgccggccag gcagcgccgg gctgttccag atcagtgcgg
     3781 ctttcatgat gcatcctccc aatgtggtga cgcagcatga tcagcataag ccgtgccagg
     3841 ctgatgacag aataatttta aaaataaaac atttgtaaaa caaatgcttg gttaattctt
     3901 tgcggccgtg caatggctgg catgagccgg gagcgggcga aaaaatattg caacgcacaa
     3961 agttgggcag ggcgcgaggt ctgcatcact cactggacgc tggcgccacg cccgccatgt
     4021 tttgcatacg cagccttaaa ttgcacaggg caatcgccgc cgtgaatcag ctcgccaagc
     4081 ccagggccag ccggcgcagc aacagccagg ggtcgtcgct ggcggcgccc ttgatggcct
     4141 gatccacctc ggcgcaggcg gccagtgcgt cggccagccg gcgcagcgac aggcggcgca
     4201 aggcgtgttc gatatggcgc tggcgctcgc cccacacccg gttgtcgcgc agcagctgcg
     4261 ccatcggccg gccttcgcgc aggccctggc ggatgcgcac caggccgcgc agctcgtcgg
     4321 ccagccgcca caccaccagc accggggttt cgccctcggc ctgcaagcca tccagcatgc
     4381 gcgcggtgcg cggcgcgtcg ccagacagcc aggcctcgga cagctgaaac acatcgtagc
     4441 gggcgacatt ggccaccgcg ctttgcaggt cggccagggt cagcgtggcg ccggcaggaa
     4501 acagcaaggc cagcttgtcc acttcttgcc gggcggccag cagattgcct tccacccgct
     4561 cgacaaaaaa ctgcatggcc tcgtcgttca gccgctgttg ctggcgcgcc agccggccgg
     4621 ccacccagtt gggcaggtgc tggcgatcca ccggctgcgc cgccagggtg acgccagcgg
     4681 cggacagcgc ttcaaaccat ttgctgttca gctgggtttt gtccagcttg ggcaacaaaa
     4741 tcagcgtcac cgtgtcggcc ggcagccgcg cggcgtagtg aatcagcgcc tcgctgccgg
     4801 cggcgcctgg cttgccgccg ggcaggcgga tttccagcag cttgcggctg gcgaacaagg
     4861 acacgctgag cgaggcttcc accagctgcg accaatcaaa atgctggctt tctacggtca
     4921 gcacttcgcg gtcgtcgtag ccctgcgcgc gggcggcggc gcggatgctg tcggcggctt
     4981 ccagcgccag cagcatctct tcaccgtgga tcacatacag cgggcccagg ccggcggcca
     5041 gctcgcgcgg cagttgctca aggctttttg ccggcatcgg gcagctcgac cacgggtttc
     5101 agcgccgcca gacggcgcac aatctgggtg gcggcgtctt cgcgcatgtc gcggaacagc
     5161 agttgttctt cctgttcctt acccagcacc tggctatcgg tgtagctgaa ctcacggcga
     5221 gtcagcacag tgattggctc gccgtagcca acgccattct tggtgacgcg cacggtgacg
     5281 cgataagtca gctcgtattc gttgaccttg ccgccacggt tgatggtgag gatatctttg
     5341 gccgtgcgct cgtcggtgac ggtgaggatg gcttcagcag cctgcggctg cgataccatc
     5401 tgcacatccg ggcggaagcg cagttggcgc tccagatgcg ggtgcagctg ggtggcgccg
     5461 gtggccagcc agacgctgga gaacggcagc gggcgcactt cgccgcccac gccgcgcagg
     5521 tggaaaccac acgcggacaa cagcagcgca gcagcggcag cgccaagcca ggtgcgtcga
     5581 ttcatcgggc gtactccgga taaaaaaatc ggtgtccaat agacaaggcg cgggcgaggc
     5641 ccgcgccaat taagagccgc taagcgccgc tcacaaaact ctcctggcgt tgttacgcgg
     5701 ccttgtcgta cttgttgtac tgccnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5821 nnnntgcggc cgcgtgccta gccaggaacg ctgcgctgag ttttgtgagc ggcgctaaac
     5881 aaaacccgcc tggcgttgtt acgcggcctt gtcgtacttg ttgtactgcc tgcggccgcg
     5941 tgcctagcca ggaacgctgc gctgagtttt gtgagcggcg ctaaaaaccc acccgccccg
     6001 gcctgcgccg gggcaaggcg tcaaaccacg atattgacca gacggcccgg caccaccacc
     6061 acctttttgg gcggctggcc ttcggtgaac ttttgcacgt tttcgttggc cagcgcggcg
     6121 gcctcgatca ctgcgcggtc ggcgtccttg gccacggtga tgctgccgcg cagcttgccg
     6181 ttgacctgca ccatcagctc gatttcgtcc tgcaccagcg cgctggcgtc caccgccggc
     6241 cagcgctggg cttccagcgt cgtgcccggg cgcagttcgc tccacagcgc gtcggtgacg
     6301 tgcggcacaa tcggcgccag cagcagcacg gcggattcca gggtttcctg cgccacggcg
     6361 cggccttgct cggcggacag cgcggttttg tccagggtgt tgagcagctc catcaccgcc
     6421 gcgatggcgg tgttgaactg ctggcgcacg ccgtaatcgt cgctgacctt ctggatggtg
     6481 ctgtgcagct tgaagcgcag ggctttttcc gccgcggtga gctcaccgcc ctgataggcg
     6541 ctggccacgc cagcggccac gtgctcgtgc acggtcttcc acagccgctt gaggaagcgg
     6601 aacgcgcctt ccacgccggc gtccgaccat tccaggctct ggctgggcgg ggcggcgaac
     6661 atcatgaaca ggcgggcggt gtcggcgccg tattgctcga tcagcgcttg cgggtccacg
     6721 ccgttgttct tggacttgga cattttttcc gtgccgccaa tcaccaccgg ctggccatcg
     6781 gcgcgcagca cggcctggat gatcttgccc ttgccgtcgc gctgcatatc gacatcggcc
     6841 gggttgatcc agtctttctt gccgtccggc agatcgcggt agaaggtgtc ggcaatcacc
     6901 atgccctggg tcagcaggcg ctggaacggc tcggtgttgt tcagcaggcc ttcgtcgcgc
     6961 atcagcttgt tgaagaagcg cgcgtacagc aggtgcagga tggcgtgttc gatgccgccg
     7021 acgtattgat cgacgttcat ccagtaattg gcgcgctcgt ccaccatgcc ggtggtgcaa
     7081 tccgggctgg cgtagcgggc aaagtaccag ctggactcga caaaggtgtc catggtgtcg
     7141 gtttcgcggc gcgccggctt gccgcagcac gggcaagtgg tttggtaaaa ctcgggcatt
     7201 ttggccagcg gcgaacccat gccgtccggc accacgtctt ccggcagcac caccggcagt
     7261 tgatcgtccg gcaccggcac gtcgccacag ctgtcgcaat ggatgatggg aatcgggcag
     7321 ccccagtagc gctggcggct gatgccccag tcgcgcaggc ggtattgggt tttcttttcg
     7381 ccggcttgct tggcggccag gtcggcgaca atggcgtcaa aggcggcgct gacgctcagg
     7441 ccgtcgtact tgccgcagtt caccgtcacc gtggcgccgt cttctttcat ggcgtaccag
     7501 tcgtgccata cggtggcgtc gaactcgggc aggccgtcgg cggtgggctt gagcacctgc
     7561 accatcggca gctggtattt gttggcaaag gcatagtcgc gttcgtcgtg cgccggcacc
     7621 gccatcaccg cgccttcgcc gtagcccatc agcacatagt tggccaccca caccggcagt
     7681 ttttcgccgg tcagcggatg gatgacgaac aggccggtgt tcatgccttt cttttccatg
     7741 gtggccatat cggcctcggc caccgagccg gatttgcact cggcgataaa cgccgccagt
     7801 tcggcattgc cctgcgcggc ctgggtggcc agcgggtgtt cggcggccac ggccacgtag
     7861 gtggcgccca tcagcgtgtc cggacgggtg gtgtacacct tcagctggcc ggcgtggccg
     7921 atgctggctt ggtcgtaggc aaaggtgatg tcggcgccaa agctcttgcc gatccagttt
     7981 ttctgcatgg tcaccacttg cggcggccag tccagcgtgt ccaggtcggc cagcagctcg
     8041 tcggcgtagg cggtgatgcg gaagtaatac atcgggatgt cgcgcttttc caccagcgcg
     8101 cccgagcgcc agccgcgccc gtcgatcacc tgctcgttgg ccagcacggt ttgatccacc
     8161 ggatcccagt tgaccacgcc gtttttcttg tagatcaagc ccttctggaa caggcgggtg
     8221 aacagccatt gctcccagcg gtagtaatcc ggcttgcagg tggtgacttc acggtcccaa
     8281 tcaatggcaa agcccaggct cttgagctgt tgcttcatgt aggcgatgtt ttcgtaagtc
     8341 cacgccgccg gggcgacgtt gtgcttcatc gcggcgtttt ccgccggcat gccaaaggcg
     8401 tcccagccca tcggttgcag cacgttaaag ccgttcagcg ccttgtagcg gctgagcacg
     8461 tcgccgatgg tgtagttgcg cacatggccc atgtgcagct tgcccgacgg gtaggggaac
     8521 atcgacaggc aatagaactt gggccggctg gtgtcttcca cggctttgaa aatctggttt
     8581 ttttcccagc gcgcctgcac ggcgggctcg atatcacgag gagagtaatg ttcttgcatg
     8641 atgggcaatg tgtcggcaat cggttacggc ggcgcacgcg ccgccaaagg cggaatcggg
     8701 cgattatacc cgatgtcgcg ccccgccatg cgccgggcaa gcggtgcatg gcggggcgcg
     8761 ggggtgggct tgatgaaaat ggccggggga gcggccagaa tagggatgag ctaaagctgg
     8821 gcgctcagtg cggcggcgtg cccagcacct cgctcaaggc ggcggcgtcc agcatggctt
     8881 cgctccaggc ttccagctgc gcggcgtcgg cgtgcgccag gcgcgccagc gcccaatctg
     8941 gcaaagggcc aaagcgccgg tcgagcaagc gctgcaaaag ctgacgctca ccctgggcga
     9001 gcccttgctg gcgcccctgc tcaatgccgc tggccatacc ttgctggatg ccctgttgca
     9061 taccttgctg catgcccagc aacatccctt gctcgcggcc ctcagccagc gcctgttctt
     9121 tccagcggtc gatattggtt tccagcatgt ccatgctctc aatcagatga tggtgttgga
     9181 tggcgtcgat gccaggcagt ttccggttca gtcgatgctg cacccagcgc atcaagactc
     9241 ggtccagcac gcgccgggcc ggcgagtggg cgatttgttc ggccagttcg cggatggcgc
     9301 gcttggcgct atccacatcg cgggtgtgtt caaaacgaaa aatcgccgcc atcacgccac
     9361 gccagcgcgc gtccagccag acgccttggt ccagcagcca gaagcgcagg ctgggctgaa
     9421 acggcgccag cgccggggga tgcggctgaa tcagcggagc aagttctgcc gggtgtgtcc
     9481 agcgtgcgtc gccattgtag agcacgatgg gcagcaccgg gggcagtcca tcgcggatat
     9541 ccatggtgtc gctgcgcagc aggtggtcgt acaaggcggc gacatattgc agcatgcgca
     9601 ccggcatgtg ggggccgctg cggctttggc attccagcag aatatacaaa aattaagagc
     9661 ggctaacaaa acccagcgaa gcgatcctgg ctaggcgcgc gaccgcagac agtacgagta
     9721 gtacggcaag gtcgtgcaac aacgccagga gggttttgtt agctgctcta aatgcgctgg
     9781 ccggctaggc ggaccgagct acaccacatc ttgttcgcgc tgcttcagcg ccggggtgac
     9841 gtagtggccg ttttcccggc gcaaggtggc gtaatcaagt tgcgccgcca ccccgggcgg
     9901 ggcgaatgtc tccagcagct cgcgcaccaa ttcagcatgg ctgaacagat atttataacc
     9961 gctgtcatga tgggcgtcgg acatggtggc atcacctggc gcagggatca tccagtgtag
    10021 cgctccatgc cgcagccagg cattcatcgc gctttatttg cgcaggcaca gcctgcttga
    10081 ttggcataaa acaccacact cagccggcaa accagacaat aggcatctgg cgtctggcgg
    10141 gctgccgccc gccaggccag gtcgcttacg ccatccccag cagcaacccc actcccgcca
    10201 ccgccagcag tgcgccgatg gcgcggctga tgcggtgcag cggcaggtac agcgccaggc
    10261 cggccaggtg cagcgcggcg gaggcggcca tgaagccggc gccgtaggcc agcaggctgg
    10321 cgctggccgg catttctgcg ccgtgggcca ggccgtgcag cagggcaaag ccgccggtca
    10381 ggcccaggcc ggcccagcgc ggcaggcgcg cgcgggcggc cagcagcagg cccagcgcca
    10441 gcaccgaggc ggcgatgccg gcttctacgc cgggcagcca cagcccggcc gcgcccagcg
    10501 cgccgcccac cgccagcagg gcgacaaagg ccagcggcgc ttgccagcgc gcggcgccgc
    10561 cttgctggcc ggcccacagg cccaccgcca gcatggccag caggtggtcg gcgccgccca
    10621 gcgggtggct caggccggcc agcatggccg agtcatggcc ggcgtggccg ctgtgcgcca
    10681 gggcggcgct gctggccagg gccagcggca ggataagcag ggatttacgc atgagacgcc
    10741 tccgggtcaa ggttgagcat gcctttttct tcgatcaagg caatgatgtc ggccaggccc
    10801 tggccggttt tcaggttgga aaacaaaaac ggccgctcgc cgcgcatggt cttggcgtcg
    10861 cgcgccatca cctccagcga ggcgcccacc agcggggcca ggtcaatttt gttgatcacc
    10921 agcaggtcag acttgcaaat gcccggccca cccttgcgcg gaatcttgtc gccggcggag
    10981 acatcaatca cgtagatggt caggtcggac aattccgggc tgaaggtggc ggccaggttg
    11041 tcgccgccgg attcaacaaa aatcacgtcc aggccaggat ggcgggcgtt cagccgggcg
    11101 acggcttcga ggttgatcga ggcgtcttcg cggatggcgg tgtgcgggca gccgccggtt
    11161 tccacgccga tgatgcgatc cggcgacagc gcctcgttgc gcaccagaaa ctgggcgtct
    11221 tcctgggtgt agatgtcgtt ggtgaccacg gcgatgttgt aacgctcgcg cagcgcggcg
    11281 cacaaagccc aggtgagatg ggtcttgccc gagcccaccg ggccgccgat gcccacgcgc
    11341 aaaggggtgg ctgacatgaa acgctcctta cagtaaaacc cgtccgccgc agacagcggg
    11401 ccgggggtct atcgtgagat aaaaaatcca tttgcccatc ggctggcgag gggcgaaaac
    11461 tcagccggct aaaaccaccg caccttgcac acggcaaccc acctcaatca cgagcggaac
    11521 agccggctgt actgggtttc gtggcgcagg gccatccagc tttgcatggg ggcgaaggcg
    11581 ctcagccggg cgtcgggcag ggtcagcgcc tgttcgctgg cggcggccag cgccggcagc
    11641 agggcggaca tcagttgctg gccggcggtc tggcccatgg gcagcgcctt catcagcacc
    11701 atcacctggt tttccagcca gccccacaga taggcttgca ccacggtggc ggcgtccagc
    11761 cccagccggc agccggccca gccgtaggcc agcaaaaacg ccagttcgcc gtcttgcagg
    11821 gcggcgggca gcggctggct ggcggtttcc ggcagggcgg ccagcagctg gcgcagcgaa
    11881 tagcccatct gcacgctttc ctgacgctgt tcgcggctgt cgcggctggc cagcagggtc
    11941 tggttcagcg ccactagcgc tggcgcgtcg ttggcggcgc tggcgcgatg ggcgcgcgcc
    12001 agcatcggcg cgtcgtagcg gctgaccggg ccggccagca ggccgcgcag ccagtgctcg
    12061 gcgctggcgc ggtcgtggat ctggcccaga tcaatggcgg tttccagccc ttgcgagtag
    12121 ctgtaagcgc caatcggcag gccggggctg gccagacgca ggcattgcag ggcggccagc
    12181 ggggtcatgc gctgtcgtcc gggctgccgt cgcggcgaaa ctggtgcagg cgcggcgcgt
    12241 actgaaacac gctgtccttg ccgtgcgagt ggtgatggcc gccgccgtag gcgccggatt
    12301 ccggctggaa cggcgcatcc agcgcctgca cgctgacgcc cagttgatcg agcatggcgt
    12361 gcagcacgct gtcttcggcc aggcgcagcc agccctcgcc cacttgcacc ggcacatggc
    12421 ggttgcccag gtggtaggcg gcgcgggtga gttcgcgcgc gtcggcggcg gtgacgtgca
    12481 gcagcggctc gggcagggcg cgcacgcgcg ccacccggcc gtcgttggcc agcaggcggt
    12541 cgccgtcggc cagcggcggg ctgccggcgg ccagaaacag cgccacgtcc tcgccgtcgg
    12601 cgctgcgggt gcgcagccgg gttttgcagc gcagctcgtg ggtcatggcc agttcggcgt
    12661 cccagtgttc gccgggctcg gcgtgtcggg tcagggtgag catgggcggc ctcagaacag
    12721 aaaataccgc tgcgccatcg gcagcacctc ggccggctcg caccacagca gctcgccatt
    12781 ggcgcgcacc tgataggttt gcggatcgac gctgatgtcc ggcagccagt tgttgtggat
    12841 caggtcggtt ttttgcacgc tgcggcagcc ctgcaccgcc accaggcgct tggccaggcc
    12901 aagttgctgg ccgatattgg cgtccagcgc ggcttgcgag acaaaggtca ccgaggtggc
    12961 ggtcatcgcc gcgccaaaag cgccgaacat cggccggtaa tgcaccggct gcggggtggg
    13021 gatgctggcg ttggcgtcgc ccatggcggc ggcggcgatc atgccgccct tgagaatcag
    13081 gctgggcttg acgccaaaaa acgccggctt ccacagcacc aggtcagcca gcttgcccac
    13141 ttcgacgctg cccacctcgt gcgccacacc gtgggtgagc gccggattga tggtgtattt
    13201 ggcgatgtag cgcttggcgc ggaagttgtc attgcccggc ccgtcgtcgg gcagcgcgcc
    13261 gcgctggcgc ttcattttgt cggcggtttg ccagcagcgc aaaatcacct cgcccacccg
    13321 gcccatggcc tggctgtcgg acgaaatcat cgaaaacgcc cccaggtcgt gcaggatgtc
    13381 ttcggcggcg atggtttccc ggcggatgcg gctttcggca aaagccacgt cttcggcaat
    13441 gctcgggtcg aggtggtgac acaccatcag catgtccaga tgctcgtcga tggtgttcac
    13501 cgtgtacggc cgggtggggt tggtcgagct gggcagcaca ttgggctggc cgcaggcctt
    13561 gatgatgtcc ggggcgtggc cgccgcccgc gccttcggtg tggtaggtgt gaatcacccg
    13621 gcccttgaag gcggcaaagg tggcttcgac aaagccggat tcgttcagcg tgtcggtgtg
    13681 gatggccacc tgcacgtcca tttcgtcggc caccgacagg cagttgtcga tggcggccgg
    13741 ggtggtgccc cagtcttcat gcagcttcag gccgatggcg ccggcgcgca cctgctcgcg
    13801 cagcggctcg ggcaggctgg cgttgccctt gccggtaaag cccaggttca tcgggaagct
    13861 gtccgccgcc ttgagcatct gcgccagatg ccacggcccc gaggtgcagg tggtggcgtt
    13921 ggtgccggtg gccgggccgg tgccgccgcc aatcatggtg gtgacgccgc tcatcagcgc
    13981 ttcttcgatt tgctgcgggc agatgaagtg aatatgggtg tcgatgccgc ccgcagtgat
    14041 aatgcagcct tcgccggcga taatctcggt ggacgcgcca atcagcagcg tcacgccggg
    14101 ttgaatatcc gggttgccgg ccttgccgat gccggcaatg cgcccggcct tcaggccgat
    14161 gtcggccttg acgatgcccc agtggtccag aatcagcgca tt
//
LOCUS       DAOPYX010000036        14085 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_4714_length_14085_cov_12.416679, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000036 DAOPYX010000000
VERSION     DAOPYX010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 14085)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14085)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14085
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4714_length_14085_cov_12.416679"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..702
                     /locus_tag="PKN55_03210"
     CDS             <1..702
                     /locus_tag="PKN55_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008952253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HNX70286.1"
                     /translation="SIKVAEAAKVIENTQRDLNIALMNELAILFEKMGIDTLDVLEAA
                     GTKWNFLKFKPGLVGGHCIGVDPYYLTHKAEKLGYIPQVILAGRRINDSMGKFIAQRA
                     IKEMIHGGSLVAGSVVTVLGLTFKENCPDLRNSRVIDVIQELREFGVQVQVCDPQAEH
                     DEAEEEYGVSLTPFAELAPADAVIVCVAHSEFAALDIPGYQKLLKPDARVLIDVKGVV
                     DKAAAQAAGLRFWRL"
     gene            715..1752
                     /locus_tag="PKN55_03215"
     CDS             715..1752
                     /locus_tag="PKN55_03215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018746502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNX70287.1"
                     /translation="MTAYDTLREQLSAQPRTWLVTGCAGFIGSNLLEALLRLGQTVIG
                     LDNFSTGHQGNLDEVRQLVGDAAWARFTFIQGDIRDADACARAVYGVDYVLHQAALGS
                     VPRSLDDPWNTHDNNINGFLRLLIASRDAGVKRFVYAASSSTYGDHPGLPKVEEQIGK
                     PLSPYAVTKYVNELYGDVFARAYGVSAVGLRYFNVFGPRQDPNGAYAAVMPMWFAALL
                     NNDTVYINGDGETSRDFCFVGNAVQANLLAATAPSLPEGHEVFNVAVGDRTTLNELYA
                     AIREQVARWRPEAAQCQPVYRDFRAGDVRHSQADISKAASLLGYAPTHRVAEGLDIAA
                     PWYAQLFGATA"
     gene            1749..2783
                     /locus_tag="PKN55_03220"
     CDS             1749..2783
                     /locus_tag="PKN55_03220"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HNX70288.1"
                     /translation="MKRVLIIRRDNIGDLVCTTPLFSALREALPNAYLAALVNSYNAE
                     ALHDNPVLDDVFVYTKAKHSERSKLRVWWDTWRLMRSLRAKQFDLVLLPSGGDQAHAL
                     KLARMIKPGKVAGFAPTKGLDVTVPADNKPQLEAERILKLLPALGLRRGKLSPVSTFA
                     RNEERAAARMALVQANIEPGPRKPLVGLHISARKPRQRWPAERFVELATRLNKATGAA
                     FMLFWSPGDEDHPQHPGDDRKAREIIAACQERNLPLLPYPTERLEQLIGGLSVCQSVI
                     CSDGGAMHLAAGLGKPMVCLFGNSDAKVWAPCGSAPFKILQPASQTVADISVDEVAAT
                     WRRLHIGQIR"
     gene            3219..3875
                     /locus_tag="PKN55_03225"
     CDS             3219..3875
                     /locus_tag="PKN55_03225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014088468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HNX70289.1"
                     /translation="MRILLVEDDLLLGDGVEAGLRQTGFTVDWIKDGVSARSALEVVD
                     YDGMVLDINLPRLSGLDLLSEIRRAGNALPVLLLTARDTVEDRILGLDAGADDYLVKP
                     FALGELVARLRAVVRRAHGRASPTLEYAGLSLDPGAHKACYQGQDIELTGKEFQLLEL
                     FLDNRNKVLSRQQIEEKLYGWNQEVASNAVEVHIHHLRKKLGAQFIRTHRGVGYQLGA
                     "
     gene            3966..5369
                     /locus_tag="PKN55_03230"
     CDS             3966..5369
                     /locus_tag="PKN55_03230"
                     /inference="COORDINATES: protein motif:HMM:NF020110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HNX70290.1"
                     /translation="MFRRLITWLNRSIHRRLLALLALGLPLLWLASAGLAYMTARYEV
                     NELFDTEQVLFAQFLASVDLRARQAGAEILPHRPQEIKDLFDDDHSGGEIDADDFAFQ
                     LRDAQGHLRLSDVRQTPLPARMDYTGFADVKIDKTVWRVYYLNDRARDLSIAVGQKLK
                     ERTSLARVLTLGQLTPWLLTMPLVLGWIWWGVRQGLRPLRSLTDQIASQQPTRLTPLA
                     AGQVPDEIAPLVHALNALLARLDEALDSERRFTADAAHELRTPLAALRVQVEVAQLAN
                     GPEARRKALGQVLAGIDRATRLVSQLLTLARLDHNPQALAGDAELVALARQHLLDASD
                     DATARNVRCEWPDSLPIWPHPVNPALPDVLLRNLVDNAVRYTPAGGRVWLEDAGQGWL
                     TLANDAPETLDAETLARLGERFFRPAGQSASGSGLGLSIARRAARLCGLELSLSLREG
                     VFRAHLRTSTTRLASRG"
     gene            5615..6745
                     /locus_tag="PKN55_03235"
     CDS             5615..6745
                     /locus_tag="PKN55_03235"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HNX70291.1"
                     /translation="MNWNACAGLALLALCVCGAARAEPAEGLLKLIVWPGYAERGQTD
                     LRYDWVTPFERETGCKVSVRVALNSDEMVNLMNQGGFDLVSASGDASLRLIFARKVAA
                     LDPARVPGWEQIEPRLRQGSGLQVAGATYGVPFVWGANMLLYDSRQFRQAPGWSAVFE
                     PQTLADGQPSQGRVQMHRGPMAIAEAALYLMKQRPALGIVDPYELSPTQYAQVLALLR
                     QQRNWVWQYWQDPEQQVHDFRQQTLALSTGWGYTARALQRQGISVRTVIPREGATGWV
                     DSWMLHSQAAHPVCAYQWMAYTLRPRVQAMVAEWYGANPANAQACALRGPAGESVCDD
                     SQHKRFADLHFWRTPQANCASQPRCVPYATWAKDYARIVAGR"
     gene            6966..8249
                     /gene="gabT"
                     /locus_tag="PKN55_03240"
     CDS             6966..8249
                     /gene="gabT"
                     /locus_tag="PKN55_03240"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009118597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009450 - gamma-aminobutyric acid catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HNX70292.1"
                     /translation="MSTTTNASLQQRKEAATPRGVGVMCGFYIDRAKNAELWDIEGRR
                     YIDFAGGIGVLNTGHLNDKVAAAVAAQAEKFSHTCYQVVPYELYVSVAEKLNAITPGD
                     HAKKTAFFTTGAEAVENAIKVARSYTGRPGVIAFFGGYHGRTNMTLGLTGKVAPYKLG
                     FGPFPGEVFHAPYPNPMHGVSEEEALKGLEKIFKCDIDPKRVAAIIIEPVQGEGGFNV
                     TPNSFLAALRAICDEHGILLIADEVQSGFARTGKMFAMDHTGVIPDLMTMAKSLAGGY
                     PLSAVTGRAEVMDAPAAGGLGGTYAGSPLALAAAEAVIEVIAEDKLCERAQLLGDKLK
                     AKLDSLKADIPHIAQARGLGAMVAVEFMKPGTNTPDAEFTKKAQQYALQHGLILLTCG
                     MYYNVMRFLFPLTIEDAVFDEALGIMEAALRHAAA"
     gene            8314..9774
                     /gene="gabD"
                     /locus_tag="PKN55_03245"
     CDS             8314..9774
                     /gene="gabD"
                     /locus_tag="PKN55_03245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014085812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009013 - succinate-semialdehyde
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0009450 - gamma-aminobutyric acid catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HNX70293.1"
                     /translation="MLNLKDPELLRQQCYIDGAWVDADNGATLDVTNPATGELVARVP
                     KMGAAETRRAIEAAERAWPAWKRKTAKERSAILRRWFELMMAAQDDLAAILTAEQGKP
                     LAEAKGEIAYGASYFEWYAEEAKRVYGDTIPTHQGDKRVVVLKDPIGVCAAVTPWNFP
                     NAMITRKAGPALAAGCPIVVKPASQTPLSALAIAVLAERAGVPAGVFSVLTGSAAAIG
                     GELTASPIVRKFSFTGSTEIGRELIAQCAPTVKKVSMELGGNAPFIVFDDADLDAAVE
                     GAMISKYRNAGQTCVCANRLYVQAGVYDAFAEKFAAAVAQLKVGNGFEAGVTQGPMID
                     EKAVGKVEEHIADALAKGGKLVAGGKRHALGGSFFEPTIITGVTQAMKVAKEETFGPL
                     APLFKFDTEADVVAMANDTEFGLASYFYSRDIGRVWRVAEQLEYGMIGINTGIISNEA
                     APFGGVKQSGLGREGSHYGMDDYLEIKYLLVGGLDR"
     gene            9989..10498
                     /locus_tag="PKN55_03250"
     CDS             9989..10498
                     /locus_tag="PKN55_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012697930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HNX70294.1"
                     /translation="MPAPILISACLLGAPVRYDGEHCRQDHPWLRALADAGRLLPICP
                     EVAGGLPTPRPPAELCGGDGHALWAGAAQVRTASGDELSAAFCAGARAACALAAQHGV
                     RLAILKARSPSCGCRQVYSGQFDGQLRAGQGVAAAALATAGVTLFDEQQLTEAARWLA
                     ANEAEHAPR"
     gene            10485..11174
                     /locus_tag="PKN55_03255"
     CDS             10485..11174
                     /locus_tag="PKN55_03255"
                     /inference="COORDINATES: protein motif:HMM:NF024676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family protein"
                     /protein_id="HNX70295.1"
                     /translation="MRRADRLYRLVDHLRGRRCVTAAQLAQWLEVSERTVYRDVRDLM
                     ASGVPIEGEAGVGYRLSRHHHLPPLMFSADELAALRLGMAMVSTWGDPALAELAANAQ
                     AKINAVLPPSAARQPAAQLFVPDLHDYPVARLTPLRRAIAARQYVELDYCRADGALSR
                     RRVRPLGLFFWGVSWTLAAWCEARDEFRNFRIDRMLACTPLADTFTPQAGRTLEDFFR
                     AVGASQPPAGG"
     gene            11247..12518
                     /locus_tag="PKN55_03260"
     CDS             11247..12518
                     /locus_tag="PKN55_03260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX70296.1"
                     /translation="MRPTLLALLLSSLLSTAALAQNPPPPPPGGPAGGPPPGAPGPQR
                     YSLEQALSDQAQLHTIAFSGLAFLTGDFASDTFLPPGKVSDYFGFQYMRDIDTASGGH
                     QGAFLTRIAHAMLALLTPEQRTLLIELARLQQPEVRRFAERRLPLIQAFRQQLDGRPL
                     SGPGALSQAAVMKYSAELYELDGKLAYQRAKVMAHIVRSLDAQQRQALARMKFGDSRT
                     WPNAPEVLDKRQFSHDVHVLVMTYASEMFSWAAGSLEADVYFCPERHGMYFGGFGMKT
                     APAIGHPDYAISTALTGDSGQALLALLTPEQRGALTELPAAQRADLAEIVRLRRQIAS
                     ELRKLLNGEPADQAKVLAASRRYGELDGRLSWRYGMAFARIQASLSPPQRAQLARLRP
                     PSGPGEPKGPFIYSEPVRNLSVGDSGFLFSR"
     gene            complement(12508..13083)
                     /locus_tag="PKN55_03265"
     CDS             complement(12508..13083)
                     /locus_tag="PKN55_03265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX70297.1"
                     /translation="MPSMRMAPRRRGWVTALLTVLAVLLFALERLIVDDLRALGRWLA
                     RRDWVARAEAWVGRLSPYPALLCFALPGLAILPVKLLAVALLSQGHVLAGVGVLLAAK
                     AAGTLAVTRIYHAAEPALLSLAWFARVRATLLHWRKVVYDSVRQQYAWRLARVQWRAW
                     RHRLRGWRQQGGQRWRRRWAAIRRRLRQLNG"
     gene            13284..>14085
                     /gene="hemF"
                     /locus_tag="PKN55_03270"
     CDS             13284..>14085
                     /gene="hemF"
                     /locus_tag="PKN55_03270"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003685602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HNX70298.1"
                     /translation="MRPELVKTWLQDLQSKIVAALEAVDGQPFVRDGWTRPTGGGGLS
                     CVIENGGVFERGGVNFSHVTGPALPPSATAARPELAGRAFEAMGVSLVLHPRNPYAPT
                     AHMNVRYFVATAEGQEPVWWFGGGMDLTPYYGEVDDAVHFHRTCQQALQPFGEHYHPQ
                     YKAWCDRYFYLKHRDEPRGVGGIFFDDLNEGGFDHCFALTQAVGEHFVPAYLPIVERR
                     CQRPYGEHERDWQAYRRGRYVEFNLVFDRGTLFGLQSGGRTESILMSMP"
BASE COUNT         2392 a         4719 c         4564 g         2410 t
ORIGIN      
        1 tcgatcaagg tggccgaagc cgccaaggtg atcgaaaaca cccagcgcga cctcaacatc
       61 gccctgatga acgagctggc catcctgttt gaaaaaatgg gcatcgacac cctggacgtg
      121 ctggaagccg ccggcaccaa gtggaacttc ctcaagttca agccgggcct ggtcggcggc
      181 cactgcatcg gcgtggaccc gtactacctc acccacaaag cagaaaaact cggttacatc
      241 ccgcaggtga tcctggctgg ccggcgcatc aacgacagca tgggcaaatt catcgcccag
      301 cgcgccatca aggaaatgat ccacggcggc agcctggtgg cgggcagcgt ggtcaccgtg
      361 ctgggcctga ccttcaaaga aaactgccct gacctgcgca actcgcgggt gatcgacgtg
      421 attcaggaac tgcgcgaatt tggcgtgcaa gtgcaagtgt gcgacccgca ggccgaacac
      481 gacgaagccg aagaagaata cggcgtgtcg ctgaccccgt ttgccgagtt ggccccggcc
      541 gatgcggtga ttgtctgcgt ggcgcacagc gaatttgccg cgctggacat ccccggctac
      601 cagaagctgc tcaagcccga cgcccgcgtg ctgatcgacg tcaagggcgt ggtggacaaa
      661 gccgccgccc aggccgccgg cctgcgcttc tggcgtctgt gaggcggcat gaccatgacc
      721 gcttacgaca ccctgcgtga acaactcagc gcccagccgc gcacctggct ggtgaccggc
      781 tgcgccggct ttatcggcag caatctgctg gaagccctgc tgcgcctggg ccagacggtg
      841 attggcctgg ataacttctc caccggccat caaggcaatc tggacgaagt gcgccagctg
      901 gtgggcgacg ccgcctgggc gcgctttacc tttatccaag gcgacatccg cgacgccgac
      961 gcctgcgcgc gcgcagtgta cggcgtggac tacgtgctgc atcaagccgc gctgggcagc
     1021 gtgccgcgct cgctggacga cccgtggaac acccacgaca acaatatcaa cggctttttg
     1081 cgcctgctga tcgccagccg tgacgctggc gtcaagcgct ttgtctacgc cgcgtccagc
     1141 tcgacttacg gcgaccaccc cggcctgccc aaggtggaag agcaaatcgg caagccgctg
     1201 tcgccgtatg cggtgaccaa atacgtcaat gagctgtacg gcgatgtgtt tgcccgcgcc
     1261 tacggcgtgt cggcggtggg cctgcgctac ttcaatgtgt ttggcccgcg ccaggatccg
     1321 aatggcgcct acgccgcggt gatgccgatg tggtttgccg cgctgctgaa caacgacacc
     1381 gtgtacatca acggcgatgg cgaaaccagc cgcgacttct gctttgtcgg caacgccgtg
     1441 caggccaatc tgctggccgc cactgcgccg tcgctgcccg agggccacga agtgttcaac
     1501 gtcgccgtcg gcgaccgcac cacgctgaac gagctgtacg ccgccatccg cgagcaagtg
     1561 gcgcgttggc ggcccgaggc ggcgcagtgc cagccggtgt accgcgattt tcgcgccggc
     1621 gacgtgcgcc actcacaagc cgacatcagc aaggccgcca gcctgctggg ctacgcgccg
     1681 acacaccgcg tggccgaagg gctggatatc gccgcaccgt ggtatgcgca actgtttgga
     1741 gccactgcgt gaagcgggtg ctgattatcc gccgcgacaa tattggcgac ctggtgtgca
     1801 ccacgccttt gttctcggcg ctgcgcgaag ccttgcccaa tgcctatctg gcggcgctgg
     1861 tgaacagcta caacgccgaa gcgctgcacg acaacccggt gctggacgat gtgtttgtct
     1921 acaccaaggc caagcacagc gaacgctcga aactgcgcgt gtggtgggac acctggcgct
     1981 tgatgcgcag cctgcgcgcc aagcagtttg acctggtgct gctgccctcg ggcggcgacc
     2041 aggcgcacgc gctgaagctg gcgcggatga tcaagcccgg caaggtggcc ggctttgcgc
     2101 cgaccaaggg cctggatgtc accgtgccgg ccgacaacaa gccgcagctg gaagccgagc
     2161 gcatcctcaa gctgctgccg gcgctgggcc tgcgccgggg caagctgtcg ccagtcagca
     2221 cctttgcccg caacgaagaa cgcgccgccg cgcgcatggc gctggtgcaa gccaatatcg
     2281 agcccggccc gcgcaagccg ctggttggcc tgcacatcag cgcgcgcaaa ccgcgccagc
     2341 gctggccggc cgagcgcttt gtcgaactgg cgactcggct gaacaaagcc actggcgcgg
     2401 ccttcatgct gttctggtcg cccggcgacg aagaccaccc gcaacacccc ggcgacgacc
     2461 gcaaggcgcg cgagatcatc gccgcctgcc aggagcgcaa cctgccgctg ttgccctacc
     2521 cgaccgaacg gctggagcaa ctgattggcg gcctgtcggt gtgtcagtcg gtgatttgca
     2581 gcgacggcgg cgccatgcac ctggccgccg gcctgggcaa gccgatggtg tgtctgtttg
     2641 gcaacagcga cgccaaggtg tgggcgccgt gcggcagcgc gccgttcaaa atcctgcagc
     2701 cggccagcca gaccgtggcc gacatcagcg tggacgaagt ggccgccacc tggcggcggc
     2761 tgcatatcgg gcagattcgc tgagtggcgc ggcggggcct gtgtgcaggc ctcgccgtgt
     2821 gtcgccgcgc gttgttttgg tcccgctgcc cccgcgcctg ctatgccggc agagccgcag
     2881 cgcggacttg catcacacac cacacgcggc ctgctggctt actgctccac ggcggatggg
     2941 ctcttctgct gtcatgccgc gtaatgtgtc ccttcctgta ctcaatggcg ttttgtcagc
     3001 cgctcttcgc cttaagccgt gattcatctt ggcgcgctat cctgcgcaat tctgtcatcg
     3061 ccgctgcccg gcatctggcg tggcagagga tggcaaaacc ttgcgcagcc tcaagtcatg
     3121 acgcttgcgt ttcacgtaga gtgcccgcat gaaagctttt gttgactgtt tgttcgcgct
     3181 tttcgccgga tccggcggtg ggtgcgtcgg aggccgccat gcgcatcctg ctggtagaag
     3241 atgacctctt gctgggcgac ggggtcgaag ccggcctgcg ccagaccggc ttcacggtgg
     3301 actggatcaa agacggcgtg tcggcgcgct cggcgctgga agtggtggat tatgacggca
     3361 tggtgctgga catcaacctg ccgcgcctgt ccgggctcga cctgttaagc gaaatccgcc
     3421 gcgccggcaa tgccttgccg gtgctgctgc tcaccgcccg cgatacggtg gaagaccgca
     3481 tcctggggct ggacgccggg gcggatgatt atctggtcaa gccgtttgcc ttgggcgagc
     3541 tggtggcgcg cctgcgcgcc gtggtgcgcc gcgcccatgg ccgcgccagc ccaacgctgg
     3601 aatacgccgg cctgagcctg gaccctggcg cgcacaaggc gtgttatcag gggcaggaca
     3661 tcgagctgac cggtaaagaa ttccagctgc ttgagctgtt tctggataac cgcaataaag
     3721 tgctgtcgcg ccagcaaatc gaagaaaaac tctacggctg gaatcaagaa gtcgccagca
     3781 acgccgtgga agttcacatc caccacctgc gcaaaaagct gggcgcacaa ttcatccgca
     3841 cccatcgcgg cgttggctat caattgggcg cctgatgccc gtcagtgggc caggctggca
     3901 acacggcctg cggggctggc atcatcaagg ccccgcgccg ttttatcctg ctgaaagcct
     3961 gccccatgtt ccgacgcctg atcacctggc tgaaccgttc catccaccgt cgcctgctgg
     4021 ccttgctggc actgggcttg ccgctgttgt ggctggccag cgccgggctg gcctatatga
     4081 ctgcacgcta cgaagtcaac gaactcttcg acaccgaaca ggtgctgttt gcccagtttc
     4141 tggcctcggt ggatttgcgc gcccgtcagg ccggggcaga aatcctgccg catcgcccac
     4201 aagaaatcaa agacctgttc gacgatgatc acagcggcgg cgagatcgat gctgatgatt
     4261 ttgcctttca gctgcgtgat gcccaagggc atctgcgctt gagtgatgtg cggcaaacgc
     4321 ccctgccggc gcgcatggat tacaccggct ttgccgatgt gaaaatcgac aaaaccgtgt
     4381 ggcgggtgta ctacctgaac gaccgcgccc gcgatttatc gattgccgtg ggccaaaagc
     4441 ttaaagagcg caccagcctg gcgcgggtgc tgacgctggg ccagcttacc ccctggctgt
     4501 tgaccatgcc gctggtgttg ggctggatct ggtggggcgt gcgccagggc ttgcgtcctt
     4561 tgcgcagtct caccgaccag atcgccagcc agcagcccac ccggctgacg ccgctggcgg
     4621 ccgggcaggt gccggatgaa atcgcgccac tggttcacgc cctgaatgcc ttgctggcac
     4681 gcttggacga agcgctggac agcgagcggc gctttaccgc cgacgccgcc cacgagctgc
     4741 gcacgccact ggcagcgctg cgcgtgcaag tggaagtcgc ccaactggcc aacggccccg
     4801 aggcgcggcg caaagccttg ggccaggtgt tggccggtat tgaccgcgcc acccgcttgg
     4861 tcagccaact gctgaccctg gcgcgtctgg accataatcc gcaggcgctg gctggcgatg
     4921 ccgagctggt ggcgctggcc cgtcagcatt tgctggacgc cagcgacgac gccaccgcgc
     4981 gcaatgtgcg ctgcgaatgg ccagacagcc tgcccatctg gccgcatccg gtgaatccgg
     5041 ccctgccgga cgttttgctg cgtaatctgg tggataacgc cgtgcgctac acccccgctg
     5101 gcgggcgggt gtggctggaa gacgccggcc aaggctggct gacgctggcc aatgacgcac
     5161 cggagacgct ggatgccgag acattggcgc gcctgggcga gcggtttttt cggccggccg
     5221 ggcaaagcgc cagcggcagc ggcctgggct tgtcgattgc ccgccgcgcc gcacggctgt
     5281 gtgggctgga attgtccctg tcgctgcgtg aaggcgtgtt tcgcgcccac ttgcgcacct
     5341 ctacgacccg attggcctcg cgcggctgag cgccgggctt gtgtgtgatc gggtgtcaaa
     5401 aaaattcaac acttctgcgg cagagtgttt aaatttttgc gatttcctgt ggtattttct
     5461 taaacgcacg cctacaatca aatacgttca tcctcgcccg gatggctgcg cgggcttggt
     5521 gctcgcgccc tggcgtgatt ccatggacat tcctgcttgg cagccaccgg caattccccg
     5581 atagtaggcg ccagtgaagc catgaaagat cgccatgaac tggaatgcat gcgctggcct
     5641 tgccctgctg gcgctgtgtg tgtgtggtgc ggcccgtgcc gaaccggctg aaggcctgct
     5701 caaactgatt gtctggcctg gttatgccga gcgtgggcaa accgacctgc gctacgactg
     5761 ggtgacgccg tttgaacggg aaaccggctg caaggtcagc gtgcgggtgg cattgaattc
     5821 tgacgagatg gtcaacctga tgaatcaggg aggctttgat ctggtcagcg cctcgggcga
     5881 cgccagcctg cgcctgatct ttgcccgcaa ggtggccgcc ctggatccgg cgcgtgtgcc
     5941 cggctgggag cagatcgagc cacgcctgcg tcagggcagc ggcttgcagg tggctggcgc
     6001 cacctatggt gtgccctttg tctggggcgc caatatgctg ttgtacgaca gtcggcagtt
     6061 tcggcaggcg ccgggctggt cggccgtgtt tgagccgcaa accctggctg atggccagcc
     6121 cagtcagggg cgtgtgcaga tgcaccgtgg gccgatggcc attgccgaag ctgcgctgta
     6181 tctgatgaaa cagcgcccgg cactgggtat tgtcgatcca tacgaattgt cgcccaccca
     6241 gtatgcccaa gtgctggcgc tgttgcgcca gcagcgcaac tgggtgtggc aatactggca
     6301 ggacccggaa cagcaggtgc acgattttcg ccagcagact ctggcgttga gcacgggctg
     6361 ggggtatacg gcgcgtgcct tgcagcgcca gggcatcagc gtgcggacag tgattccgcg
     6421 cgaaggcgcc actggctggg tggacagctg gatgttgcac agtcaggccg cacacccggt
     6481 gtgcgcctat cagtggatgg cctataccct gcgcccgcgt gtgcaggcca tggtggctga
     6541 atggtatggc gccaatccgg ccaatgccca ggcgtgcgcc ctgcgcgggc cggcgggtga
     6601 gtcggtgtgt gacgacagcc agcacaagcg ttttgccgat ttgcacttct ggcgcacgcc
     6661 acaggccaat tgcgccagcc agccgcgttg tgtgccctat gctacctggg ccaaggatta
     6721 tgcccggatc gtggcgggcc gctgacgccg ggcagccagt gttgttttac tgtttttgtc
     6781 ggttttttgt agagggaatc gccggccaac tggtcggcgc gtatccgtag accgccacag
     6841 gcggcatgag ccagcagcaa ggctccggaa cccacgcgcg agccgggtgg ccgcgcgcgt
     6901 tgtcatcatg atgtcttgac ccaacacacg gggcggcgcc gccgccgcac gggagaaccc
     6961 atacaatgag caccaccacc aacgccagcc tgcaacaacg caaagaagcc gcaaccccgc
     7021 gcggggtcgg cgtgatgtgc ggtttttaca tcgaccgcgc caaaaacgct gaactctggg
     7081 acatcgaagg tcgccgctac atcgactttg ccggcggcat tggcgtgctg aacaccggcc
     7141 acctgaacga caaagtggcc gccgccgtgg ccgcccaagc cgagaaattc agccacacct
     7201 gctatcaggt tgtgccctac gagctgtatg tgtcggtggc ggaaaaactc aacgccatca
     7261 ccccgggcga ccacgccaag aaaaccgcgt tcttcaccac tggcgccgaa gccgtggaaa
     7321 acgccatcaa ggtggcgcgc agctacaccg gccgtccggg cgtgatcgcc ttctttggcg
     7381 gctaccacgg tcgcaccaat atgaccctgg gcctgaccgg caaagtggcc ccgtacaaac
     7441 tgggctttgg ccccttcccg ggcgaagtgt tccacgcccc ctacccgaac ccgatgcacg
     7501 gcgtgagcga agaagaagcg ctcaagggcc tggaaaagat tttcaagtgt gacatcgacc
     7561 ccaagcgcgt ggcggccatc atcatcgagc cggtgcaagg cgaaggcggc tttaatgtca
     7621 ccccgaacag cttcttggcc gcgctgcgcg ccatctgtga cgaacacggc atcctgctga
     7681 tcgccgacga agtgcaatcc ggctttgccc gcaccggcaa gatgttcgcc atggaccaca
     7741 ccggcgtgat ccccgacctg atgaccatgg ccaagtcgct ggctggcggc tacccgctgt
     7801 ccgccgtcac cggccgcgcc gaagtgatgg acgccccggc cgcaggcggc ctgggcggca
     7861 cctacgccgg cagcccgctg gccctggccg ccgccgaagc ggtgattgaa gtcatcgccg
     7921 aagacaagct gtgcgaacgc gcgcaactgc tgggcgacaa gctcaaggcc aagctggaca
     7981 gcctgaaggc tgacattccg cacatcgccc aagcccgtgg cctgggcgcc atggtggcag
     8041 tggaattcat gaagccgggc accaacaccc cggatgctga gttcaccaag aaagcccagc
     8101 aatacgcgct gcaacacggc ctgatcctgc tgacctgcgg catgtactac aacgtgatgc
     8161 gcttcctgtt cccgctgacc atcgaagacg ccgtgttcga tgaagccctg ggcatcatgg
     8221 aagccgcgct gcgccacgcc gccgcctgat tctgccgccc gccggcccac ccactgggtg
     8281 ggccgtttgc ctgtcatcac cggaaagccc gacatgctga atctgaaaga ccccgagctg
     8341 ctgcgtcagc agtgctatat cgacggcgcc tgggtggatg ccgacaatgg cgccaccctg
     8401 gatgtgacca atccggccac tggcgaactg gtggcgcgcg tgccgaaaat gggcgccgcc
     8461 gaaacccgtc gcgccatcga ggccgccgaa cgcgcctggc cggcctggaa gcgcaaaacc
     8521 gccaaagaac gcagcgccat cctgcgccgc tggtttgaac tgatgatggc cgcccaggac
     8581 gacctggccg ccatcctcac cgccgaacaa ggcaagccgc tggccgaagc caagggcgaa
     8641 atcgcctacg gcgccagcta cttcgagtgg tacgccgaag aagccaagcg cgtgtacggc
     8701 gacaccatcc ccacccacca gggcgacaag cgcgtggtgg tgctgaaaga cccgattggc
     8761 gtgtgcgccg ccgtcacccc gtggaacttc cccaacgcca tgatcacccg caaggcaggc
     8821 ccggcgctgg ccgccggctg cccgatcgtg gtcaagcccg cctcgcaaac tccgctgtcg
     8881 gcgctggcga ttgccgtgct ggccgagcgt gctggcgtgc cggctggcgt gttcagcgtg
     8941 ctgaccggtt ccgctgccgc gattggcggc gagctgaccg ccagcccgat tgtgcgcaag
     9001 ttctcgttta ccggctcgac cgaaatcggc cgcgaactga ttgcccaatg cgcgccgacg
     9061 gtgaaaaaag tctcgatgga gctgggcggc aacgcgccgt tcatcgtgtt tgacgacgcc
     9121 gacctggacg cggcggttga aggcgcgatg atctccaaat accgcaacgc cgggcaaacc
     9181 tgcgtctgcg ccaaccgcct gtatgtgcag gccggcgtat acgacgcctt cgccgaaaaa
     9241 ttcgccgccg ccgtggccca gctcaaggtc ggcaacggct ttgaagccgg cgtcacccaa
     9301 ggcccgatga tcgacgaaaa agccgtcggc aaagtggaag aacacatcgc cgacgccctg
     9361 gccaagggcg gcaagctggt ggccggcggc aagcgccatg ccctgggcgg cagcttcttt
     9421 gagccgacca tcatcaccgg cgtgacccag gcgatgaaag tggccaagga agaaaccttc
     9481 ggcccgctgg ccccgctgtt caagttcgac accgaagccg acgtggtggc catggccaat
     9541 gacaccgaat tcggcctggc cagctacttc tacagccgcg acatcggccg cgtctggcgg
     9601 gtggccgagc agctggaata cggcatgatc ggcatcaaca ccgggattat ctccaacgaa
     9661 gccgcgccgt ttggcggggt gaagcaatcc ggcctcggcc gcgaaggctc gcactacggc
     9721 atggacgact atctggaaat caaatacctg ctggtcggcg gtctggaccg ctaagcgctg
     9781 ctgaccaagc cgtgttgcgt gtactgtctg ctcgtgtgcc tggcaggcac gctgcgctga
     9841 gtttggtgag cacccgtttg atctttcctg tgtgaccccg tgagcctgaa acggcgcgcc
     9901 ctgttgggtg cgccgttttt ttgcgtcgct gctggctgcg cgggtatgct ggccgccgtt
     9961 gaccccgcgc cacaagaaag cccctgccat gcccgccccc atcctgatca gcgcctgcct
    10021 gctgggcgcg cccgtgcgct acgacggcga gcactgccgc caggatcacc cctggctgcg
    10081 cgcgctggcc gacgccggcc ggctattgcc catctgcccg gaagtggccg gcggcctgcc
    10141 caccccgcgc ccgccggccg agctgtgcgg cggcgacggc cacgcgctgt gggccggcgc
    10201 cgcgcaggtg cgcaccgcca gcggcgacga gctgagcgcg gcgttttgcg ctggcgcccg
    10261 cgccgcctgc gcgctggccg cacaacacgg cgtgcgactg gccatcctca aggcgcgcag
    10321 cccgtcctgc ggctgtcggc aggtgtacag cggccagttt gacggccaac tgcgcgccgg
    10381 ccagggcgtg gccgccgccg cgctggcgac ggccggggtg acgctgtttg atgaacagca
    10441 actgactgag gccgcgcgct ggctggcggc aaacgaggcc gagcatgcgc cgcgctgacc
    10501 gcctgtatcg gctggtggac catctgcgcg ggcggcgttg cgtcaccgcc gcccagctgg
    10561 cgcaatggct ggaagtctcc gagcgcacgg tgtaccgcga tgtgcgtgac ctgatggcca
    10621 gcggcgtgcc gattgaaggc gaggccggcg tcggctatcg gctgtcgcgc catcatcacc
    10681 tgccgccgct gatgttcagc gccgacgagc tggccgcgct gcggctgggc atggccatgg
    10741 tgtccacttg gggcgacccg gcgctggccg agctggcggc caatgcccag gccaagatca
    10801 acgccgtgct gccgcccagc gccgcgcgcc agccggcggc gcagctgttt gtgccggatt
    10861 tgcacgatta cccggtggcg cggctgacgc cgctgcgccg ggcgattgcc gcgcgccagt
    10921 atgtcgagct ggattattgc cgcgccgatg gcgcgctcag ccgtcggcgg gtgcgcccgc
    10981 tggggctgtt tttttggggg gtgagctgga cgctggccgc ctggtgcgag gcgcgcgatg
    11041 agtttcgcaa ttttcgcatc gaccgcatgc tggcctgcac gccgctggcc gacaccttca
    11101 ccccgcaagc cgggcgcacg ctggaggatt tttttcgggc ggtgggcgcc agccagccgc
    11161 ccgctggcgg ctgatgggcc catcaagcgc cgggcgcgca tgccgatagc aaaatattcg
    11221 ccctgactaa tcccgaaagc gtgcccatgc gccccaccct gctggctctg ttgttgtcct
    11281 cgctgctgtc cactgccgcc ctggcgcaaa acccaccgcc gccgccgccg ggcggcccgg
    11341 ctggcggccc gccgcctggc gcgccgggtc cgcagcgcta ttcgctggag caggcgctgt
    11401 ccgaccaggc gcagctgcac accatcgcct tcagcggcct ggcctttttg accggggact
    11461 ttgccagcga cacctttttg ccgccgggca aggtgtcgga ttattttggc tttcagtata
    11521 tgcgcgacat cgacaccgcc agcggcggcc atcaaggcgc gtttctcacc cgcatcgccc
    11581 acgccatgct ggccttgctc acccctgagc agcgcacgct gctgattgag ctggcgcggc
    11641 tgcaacagcc cgaggtgcgc cgctttgccg agcgccgctt gcccttgatt caggcgtttc
    11701 gccagcagct ggacggccgg ccgttgtccg ggccgggggc tttgtcgcag gcggcggtga
    11761 tgaaatactc cgccgagctg tacgagctgg acggcaagct ggcctatcag cgcgccaagg
    11821 tgatggcgca tattgtgcgc agcctggacg cccagcagcg ccaggcgctg gcgcgcatga
    11881 agtttggcga ttcgcgcact tggcccaatg cgccggaagt gctggacaag cgccagtttt
    11941 cgcacgatgt gcatgtgctg gtgatgacct acgccagtga gatgttctcc tgggcggccg
    12001 gctcgctgga ggcggatgtg tatttttgcc ccgagcgcca cggcatgtat tttggcggct
    12061 ttggcatgaa aaccgccccg gcgattggcc acccggatta cgccatcagc accgcgctga
    12121 ccggcgacag cggccaggcg ctgctggccc tgctcacccc cgagcaacgc ggcgcgctga
    12181 ccgagctgcc cgccgcccag cgcgccgacc tggccgagat tgtgcgcctg cgccggcaaa
    12241 tcgccagcga gctgcgcaaa ctgctgaacg gcgagccggc cgaccaggcc aaggtgctgg
    12301 cggcctcgcg ccgctatggc gaattggatg gccgcctgtc ctggcgctat ggcatggcct
    12361 ttgcccgcat tcaggccagc ctgagcccgc cgcagcgcgc ccagctggcg cggctgcgcc
    12421 cgccaagcgg gccaggcgag ccgaaagggc cgtttatcta ttccgagccg gtgcgcaatc
    12481 tgtcggtggg ggattccggg tttttgttca gccgttaagc tgacgcagcc ggcgacggat
    12541 ggccgcccag cgccgtcgcc agcgctggcc tccctgctgg cgccagccgc gcaaccggtg
    12601 ccgccaggcg cgccactgta cgcgggccag ccgccaggcg tattgctggc gcacgctgtc
    12661 gtataccacc ttgcgccagt gcaatagcgt ggcgcgcacc ctggcaaacc aggccaggct
    12721 gagcagcgcc ggctcggccg cgtggtaaat ccgcgtgacc gccagcgtgc ctgccgcctt
    12781 ggccgccagc agcaccccca cccccgccag cacatgaccc tgcgacagca gggccaccgc
    12841 cagcagtttc accggcaaaa tcgccagccc cggcagcgca aagcacagca aggccggata
    12901 cggcgacaag cgccccaccc aggcctcggc gcgcgccacc cagtcacggc gcgccagcca
    12961 gcgccccagc gcgcgcaagt catcgacaat caggcgctcc agggcaaaca gcagcaccgc
    13021 cagcaccgtc aacagggcgg tgacccagcc gcgccggcgc ggcgccatac gcatagacgg
    13081 catgatgtgt tctcagcaac agaatgcggt gcagtgtgag gggcgtgcgg taaaaagcaa
    13141 gtgccgtggg tgaatttccg tctcgccgct cccggcgcat ggccatggtt gtccgcctgg
    13201 ccggcacggt ccgctggcgg cggtgatcca ggggccgcca cgcgctacaa tggccgccca
    13261 atcccccctg actggagcct gtcatgcgtc ccgaacttgt caaaacctgg ctacaggact
    13321 tgcaaagcaa gattgtcgcc gcgctggaag ccgttgacgg ccagcctttt gtccgcgacg
    13381 gctggacccg ccccactggc ggtggcgggc tgtcctgcgt gatcgaaaac ggtggcgtgt
    13441 tcgagcgcgg cggcgtgaat ttctcgcacg tcaccggccc ggcgctgccg ccctcggcca
    13501 ccgccgcccg ccccgagctg gccggccgcg cctttgaagc catgggcgtg tcgctggtgc
    13561 tgcacccgcg caacccctac gcccccaccg cccacatgaa tgtgcgctac tttgtcgcca
    13621 ccgccgaagg ccaggagccg gtgtggtggt ttggcggcgg catggacctc accccgtatt
    13681 acggcgaggt ggacgacgcc gtgcattttc accgcacctg ccaacaggcg ctgcagccgt
    13741 ttggcgaaca ctaccacccg caatacaaag cctggtgcga ccgctatttt tacctcaagc
    13801 accgcgacga accgcgcggc gtgggcggga ttttctttga cgacctgaac gaaggcggct
    13861 ttgaccactg ctttgccctc acccaggcgg tgggcgaaca ctttgtgccc gcctacctgc
    13921 ccatcgtcga acgccgctgc caacgcccct acggcgagca cgaacgcgac tggcaagcct
    13981 accgccgtgg ccgctatgtg gaattcaacc tggtgttcga ccgtggcacc ctgttcggcc
    14041 tgcaatccgg cggccgcacc gaatccatcc tgatgtcgat gccgc
//
LOCUS       DAOPYX010000037        13977 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_4762_length_13977_cov_8.240698, whole genome shotgun sequence.
ACCESSION   DAOPYX010000037 DAOPYX010000000
VERSION     DAOPYX010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 13977)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13977)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13977
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4762_length_13977_cov_8.240698"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..949
                     /gene="dxr"
                     /locus_tag="PKN55_03275"
     CDS             <1..949
                     /gene="dxr"
                     /locus_tag="PKN55_03275"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008953647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HNX70299.1"
                     /translation="TEVLYGAEALAQVATLPEVDAVMAAIVGAAGLPPTLAAVRAGKR
                     VLLANKESLVMAGELFMAEVRASGSVLLPIDSEHNAIFQSLPDDYRGDLAAAGVERIV
                     LTASGGPFRGWRAEQLADVTPAMAVKHPNWVMGRKISVDSATLMNKGLEVIEARWLFN
                     AAPDAIEVVVHPQSVIHSMVRYRDGSVVAQLGTPDMRTPIAHALAWPQRIDAGVAPLD
                     FSTLSGLTFEAPDTLNFPCLRLAFDALHAGGNAPAVLNAANEIAVAAFLDGALPFTGI
                     PGLVDAALARFAGQPCASVEALLSCDGEVRAFCRARLAV"
     gene            953..2293
                     /gene="rseP"
                     /locus_tag="PKN55_03280"
     CDS             953..2293
                     /gene="rseP"
                     /locus_tag="PKN55_03280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014087620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HNX70300.1"
                     /translation="MLSTILAFAVAIGVLVTFHEFGHYWVARRCGVKVLRFSIGFGRP
                     LFSVRWRGTEWSISPIPLGGYVKMLDEREMDVPAHERHLAFNAQPVSRRMAIVAAGPL
                     ANLLLAVLLYMALFMSGVNWIKPGVGSVQPGSPAAQAGLRGGEVIAAVNGEAVESWQD
                     LRLALMEGLADKAITLTLSQPTTQQLTVPPAALAELEADNHGLARLGLSPARPLAEIA
                     MVDANGAGARGGLQVGDRLLALDGQPLRGWEDFSRQVRQHPGQPLMLTVSRHGRELTL
                     RLTPDSLAGPDGQRLGRLNVAPRQDEAWMNSLRMSRQDDPISALAHAVEKTWDTAWSS
                     FSMIGRMVLGQLSMDNLSGPLMIADYAGQSARQGTQSYLEFMALISISLAVLNLLPIP
                     VLDGGHLMYYTVELLRGRPVSERTMALGQRIGLAALLGLMTFALFNDIQRLVTG"
     gene            2336..4594
                     /gene="bamA"
                     /locus_tag="PKN55_03285"
     CDS             2336..4594
                     /gene="bamA"
                     /locus_tag="PKN55_03285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014087619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HNX70301.1"
                     /translation="MGLSLAPLAWAAEPFVIKDIRVEGLQRTEPGTVFNYLPVKVGDT
                     FSDDKAESAIKALFATGFFADVRIETENNVVIVAIAERPVIAELNINGSKEFDSKQLK
                     KALKDNGLAESRVFDKSVLEQAEQELKRQYYSKGKYSVQINTKVTKLERNRVAVTMDI
                     DEGVAARIKQIKIVGTKAFKEGDLLDEFALTDSGWLTWLTRDDQYSKQKLQGDLERLR
                     SYYLNRGYFEFNIDSTQVSISPDKRDVYITVNVSEGEKYTVSDVRFAGDLTVPEATLK
                     PLVQIKPGETFTRDKVNDTVSAITDLLGNSGYAFANVNAVPEVDKDKHQVAFTFFVDP
                     GRRVYVRRINVAGNTRTRDEVIRREMRQMESGWYDNQKIKRSKERLDLLGYFTDVNVE
                     TPAVTDTPDQVDVNVSVTEKPTGSVQLGAGFSQDNGFALSASISQTNFFGSGKAVSAG
                     FNTDKVNEFYNLSFTDPYFTVDGTSLGYDVYRKTYDPSQKDRGQYKTKTDGIMVRAGV
                     PITEFDRINFSAGVERTKIGLFSDSPKRYVDFVNEYSSSNFSLLASASWARDTRDSAL
                     WPTKGSVSKAAAEFALPGGDVQYYRFSVGNQWYYPLSKNLTLMLNGELGMVNSWSSKK
                     LPFYENFYLGGISSVRGFESGTIGAQDENNNAYGGTRRAVFNAEVLFPFPGLRDDKTL
                     RLSTFFDVGTVFGGQFNSGLTWRDNLRYSAGLGLSWVSPLGPMRFSFSQPLHKKSNDH
                     LERFQFTLGTSF"
     gene            4602..5111
                     /locus_tag="PKN55_03290"
     CDS             4602..5111
                     /locus_tag="PKN55_03290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012697752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HNX70302.1"
                     /translation="MSKLALLWRALMASLVLAMLWLAAPAMADELRLGYVDTERVYRE
                     ATAAVKAQQRLKAEFAPREAELADMARRARELKQTLDASKLSDAERRTRERELQALDR
                     DFQAKQLEFRQEFGQRRTEEFAAIQQRANQVIRDLARSERFDLILQDVVYVSPRFDIT
                     ERVLKALDD"
     gene            5200..6255
                     /gene="lpxD"
                     /locus_tag="PKN55_03295"
     CDS             5200..6255
                     /gene="lpxD"
                     /locus_tag="PKN55_03295"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008953651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HNX70303.1"
                     /translation="MAWTLSEIVARLGGELHGEDRSVARLAPLDEAGADEIAFVTGAK
                     HADALRASAAGAVIVKADLLDIAARPAIVCADPYVYFARLAGLMCPPAAARAAGAHPS
                     AAVAADAQVAASAEIREFVSIGAGAVIGERVLLMAGAVVEAGAVIGDDCVLHPRVVVH
                     HGCVVGARCILHAGAVIGADGFGNAWAGDHWEKIPQIGRAVLGDDVEIGANTTIDRGA
                     LADTVIERGVRLDNLIHIAHNCRVGAHTAMAACVGVAGSTSIGANCMIGGAAMISGHL
                     KIGDRVHISGGTLVAKNLDKPGQYTAVYPLATHKEWLGNAAHVRQLGKLAARVKQLEA
                     RLAAANATDPHSTKEPQ"
     gene            6252..6710
                     /gene="fabZ"
                     /locus_tag="PKN55_03300"
     CDS             6252..6710
                     /gene="fabZ"
                     /locus_tag="PKN55_03300"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012697750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HNX70304.1"
                     /translation="MSEFATLIDLRAILKLLPHRYPFLLVDRVTELEVGKRIVALKNV
                     TINEPFFVGHFDGYPVMPGVLIVEALAQAAGILAVKTHDGDVRQENALYFFVGIDNCR
                     FKRQVIPGDQLTLEVEMLRVARGIGKFRAVAKVDGEIAAEADLMCARREV"
     gene            6714..7487
                     /gene="lpxA"
                     /locus_tag="PKN55_03305"
     CDS             6714..7487
                     /gene="lpxA"
                     /locus_tag="PKN55_03305"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008953653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HNX70305.1"
                     /translation="MAIHPTAIIAPGAQIDASVEIGPYAVIGEHVSIGAGTRVGPHTV
                     IEGHTTIGRQNQIFQFASLGAAPQDKKYAGEPTRLEIGDNNTIREFCTFNVGTVQDGG
                     LTRLGNDNWVMAYVHIAHDCLVGDHCILANNATLAGHISIGDHVFLGGLTAVHQFCTI
                     GAHAMTAGGSIVVQDIPPYVTAAGNHAHPAGINSEGLRRRGFDAEQISRIKRAYKQIY
                     RQGLSLEDAKAAITEAAADAPELALFVDFFARAKRGIIR"
     gene            7487..8656
                     /gene="lpxB"
                     /locus_tag="PKN55_03310"
     CDS             7487..8656
                     /gene="lpxB"
                     /locus_tag="PKN55_03310"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018747500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HNX70306.1"
                     /translation="MGLFPPDAGLRIAMVAGEASGDMLGARLIDAIRARRPDARFVGI
                     GGPKMQAAGLESLFPQETLAVRGYVEVLRNLRAILAIRRELKAKLLAERPDVFIGVDA
                     PDFNLAVEHSLKAAGIRTIHYVSPSIWAWRGERVHKIKQAVNRVLALFPMEPPIYQAA
                     GVPVSYVGHPLAESMPIVPDRQGAREVLNILPRQAPVFTLMPGSRQGEVDVMAPLFIA
                     AARLIAKEVPDAQFLVPLATRPTFDRFEQLLWRLKAHQLPIRRLFGHAQHAMAAADAV
                     LVASGTATLEVALAKRPMVISYKISPITYRIVKRKLQLPYVGLPNILCGEFIVPELLQ
                     DDATPENLAQALLNAWRDADYRAWLDGKLTELHHTLKQDSAARAAQAVLEVAEHG"
     gene            8649..9251
                     /gene="rnhB"
                     /locus_tag="PKN55_03315"
     CDS             8649..9251
                     /gene="rnhB"
                     /locus_tag="PKN55_03315"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011135758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HNX70307.1"
                     /translation="MAEQQNRLCGVDEAGRGPLAGAVFAAAVILDPTRPISGLADSKK
                     LSEARRDALALEIREKALAWAIASASVEEIDQINILHASMLAMARAVDALAVTPSEVQ
                     VDGNRTPKLFKPLPVSAIVKGDAKVAEISAASILAKTARDAELVALDAQYPAYGFARH
                     KGYPTAEHLAAIAEHGVLSIHRKTFGPVKAWLATHQGELF"
     gene            9251..9763
                     /locus_tag="PKN55_03320"
     CDS             9251..9763
                     /locus_tag="PKN55_03320"
                     /EC_number="3.2.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011135759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033958 - DNA-deoxyinosine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-deoxyinosine glycosylase"
                     /protein_id="HNX70308.1"
                     /translation="MAAVGEGAALARCFAPVVNADTQVLILGSLPGAASLAAQQYYAH
                     PRNQFWPLLAAVLKQPDLPALAYPARLQALLAAGVGLWDVVAQAQRRGSLDANIRDAQ
                     ANPLPALIAQLPRLHTVAFNGATAARAAVQLTPYPWLRVLPLPSSSPAHTLAFADKLA
                     AWQALGDDIH"
     gene            complement(9829..10845)
                     /locus_tag="PKN55_03325"
     CDS             complement(9829..10845)
                     /locus_tag="PKN55_03325"
                     /inference="COORDINATES: protein motif:HMM:NF012659.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX70309.1"
                     /translation="MILSRSSLALTLALVLGGLTAQAHAAGYAGAFSAEQACTATVNK
                     PKAGKPDNNPDQFTVQSGVVYPIIDADAPNAPTWYRIQAQGAQPAERWVSAHCGRGAL
                     TSRPASTSSTSSASSGKACNLAGQGDSYVFAVSWQAAFCETHQSKPECQQPTPWASQN
                     FTLHGLWPNLGRCGTNYGYCSSQPQQKNFCDYPEPAISASTMRQLGEVMPSAAAGSCL
                     QRHEWFKHGTCQTEWDADGYFEVAIDLVKQFNASGVGPFIAANRGLSIPTQDLFAVID
                     QGLGANAHKRMKFFCTNGQLVDIYMNLPDSIPRQQPKLGPLLQAAKEGFSSSCGESFR
                     VDVM"
     gene            complement(10965..11138)
                     /locus_tag="PKN55_03330"
     CDS             complement(10965..11138)
                     /locus_tag="PKN55_03330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX70310.1"
                     /translation="MLTAFFSCPPRASVQHVTGEKARNQVWEYDSTGALKRQEVESRR
                     ANRSFLLMLEGKW"
     gene            complement(11266..12060)
                     /locus_tag="PKN55_03335"
     CDS             complement(11266..12060)
                     /locus_tag="PKN55_03335"
                     /inference="COORDINATES: protein motif:HMM:NF014376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNX70311.1"
                     /translation="MPLGPDWRAVDLPEIDTLPSPVFLRSQRLPARHSFLPHTHRWRQ
                     LVYATAGALLVQAPDTWCVITPEQAVWLPTGVLHATGALHDAEFRNLYVDAALALDMP
                     EHCQVLAATPLLRALIVELDAIGQRQESADYTARVQQLIVDQLPRLPRQDVHLPWPKD
                     PQLRTLCEALYAQPADPRDLPAWGAALGASARTLSRRFERELGISLRDWRTRLRLIRA
                     LEWLGAGRAVTDVALELGYASPSAFSHMFRQAMGCSPSDWRRGECG"
     gene            12158..13342
                     /locus_tag="PKN55_03340"
     CDS             12158..13342
                     /locus_tag="PKN55_03340"
                     /inference="COORDINATES: protein motif:HMM:NF018485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfB/YjiJ family MFS transporter"
                     /protein_id="HNX70312.1"
                     /translation="MSIAVPLDPALASARWRVLAAGVCALILTVGLARFAYTPMLPIM
                     RAQAGLSDLAGGWLATFNYVGYISGALLAATLSRLQDKFRLYRLGLALAVLTTLGMGL
                     TDNLYLWTALRLLAGISSTAGLLIASGLVLNWLIRHGCRAELGLHFTGIGLGIVVSGL
                     AVAAMASLDWAGQWLGLGVLGLVFMLPAWWWMPAPAGAGATATAPAAGPSRRWMGFFV
                     AAYFCAGFGYVISATFIIAIVGKLPLLANLGGWVWVLVGLMATPSCFVWDRLAARLGQ
                     IPALLLAYGLQTVSIALPAASEGALLNLLSAALYGGTFVGIVSLTLSIIGRHFPANPA
                     KAMARMTLSYGVAQIVAPALAGYIATVTGSYRGALWLAAAVMLAGMAALAALREKPAH
                     RP"
     gene            13583..13903
                     /locus_tag="PKN55_03345"
     CDS             13583..13903
                     /locus_tag="PKN55_03345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX70313.1"
                     /translation="MYDCQSDSYADFTVAQQTTTTTTQALRRPEPLAGDVRVRALPGH
                     EAVLTELAISAERQGYLAERRGARLTLFAVTLADVLAFNRAFATRATFSAEFRLAAPV
                     AFHG"
BASE COUNT         2439 a         4527 c         4517 g         2494 t
ORIGIN      
        1 caccgaagtg ctgtacggcg ccgaggcgct ggcgcaggtg gccaccctgc cggaagtaga
       61 cgcggtgatg gccgccattg tcggcgccgc cggcctgccg cccaccctgg cggcggtgcg
      121 cgccggcaag cgcgtactgc tggccaataa agaatcgctg gtgatggccg gcgagctgtt
      181 tatggccgag gtgcgcgcca gcggcagcgt gctgctgccg attgacagcg agcacaatgc
      241 gattttccag tcgctgccgg acgactatcg cggcgatctg gccgctgccg gcgtcgagcg
      301 catcgtgctg accgccagcg gcgggccgtt ccgcggctgg cgcgccgagc aactggccga
      361 tgtcaccccg gccatggcgg tgaaacaccc caattgggtg atgggccgca agatttcggt
      421 ggattccgcc accctgatga acaagggcct ggaagtgatc gaggcgcgct ggttgttcaa
      481 tgccgcgccg gacgccatcg aagtggtggt gcatccgcaa agcgtgattc actcgatggt
      541 gcgctaccgc gatggctcgg tggtggcaca gctgggcacg ccggacatgc gcacgccgat
      601 tgcccatgcc ctggcctggc cgcagcgcat cgacgcgggc gtcgccccgc tggatttctc
      661 caccctgtcc gggctgacct tcgaagcgcc ggacacgctg aactttccct gcctgcgcct
      721 ggcgtttgac gcgctgcacg ccggcggcaa cgccccggcg gtgctcaacg ccgccaatga
      781 aatcgccgtc gccgccttcc tggatggcgc gctgccgttc accggcattc ccggcctggt
      841 agatgctgcg ctggcgcgct ttgccggcca gccctgcgcc agtgtcgaag cgctgctgag
      901 ctgcgatggc gaagtgcgcg ccttctgtcg cgcccgactg gcggtctgag gcatgctgag
      961 cacaattctc gcctttgccg tggccatcgg cgtgctggtg acctttcatg aatttggcca
     1021 ctattgggtg gcgcgccgtt gcggcgtgaa agtgctgcgt ttttccattg gttttggccg
     1081 gccgctgttc agcgtgcgct ggcgcggcac ggaatggagc atctcgccga ttccgctggg
     1141 tggttacgtc aagatgctgg acgagcggga aatggacgtg ccggcgcacg agcgccatct
     1201 ggcttttaat gcccagccgg tcagccggcg catggcgatt gtcgccgccg gcccgctggc
     1261 caatctgctg ctggcggtgc tgctgtatat ggcgctgttc atgtctggcg ttaattggat
     1321 caagccggga gtgggttcgg tacaaccggg ctcgccagcg gcgcaggcgg gcctgcgtgg
     1381 cggcgaggtg attgccgcag tgaatggcga agccgtggaa agctggcagg acttgcgcct
     1441 ggcgctgatg gaaggcctgg cggataaagc catcaccctg acgttaagcc aacccaccac
     1501 gcaacaactg accgtgccgc cggcggcgct ggctgaactg gaagccgaca accacggcct
     1561 ggcccggctc ggcctgtcgc ctgcgcggcc gctggcggaa atcgccatgg tggacgccaa
     1621 tggcgctggc gcgcgcggcg gcctgcaagt gggcgaccgc ctgttggcgc tggatggcca
     1681 gccgctgcgc ggctgggagg atttttcccg gcaggtgcgc cagcaccctg gccagccctt
     1741 gatgctgacc gtgtcgcgtc atggccgtga attgacgctg cgtttgacgc ccgacagcct
     1801 ggccgggccg gatggccagc gccttggccg gctgaatgtg gcgccgcgcc aggacgaagc
     1861 ctggatgaat agcctgcgca tgagccgcca ggacgacccg atcagcgcac tggcgcacgc
     1921 ggtggaaaaa acctgggaca ccgcctggag cagcttttcc atgatcggcc gcatggtctt
     1981 gggacagttg tcgatggaca atctgtccgg cccgctgatg attgccgact acgccggcca
     2041 gagcgcgcgc cagggcacac aatcgtatct ggaattcatg gcgctgatca gcatcagcct
     2101 ggctgtgctc aatctgctgc cgattccggt gctcgacggg gggcacttga tgtattatac
     2161 cgtcgagcta ttgcgcgggc gtccggtttc cgaacgcacc atggccctgg gtcagcgcat
     2221 cgggctggcc gcgctgcttg gcctgatgac gtttgcgctt ttcaacgaca ttcaacgtct
     2281 cgtgacgggt taaccgccaa tctctatgaa aatcaagaca ctagttctct ccatcatggg
     2341 tctgtccctg gcgccgctgg cctgggcggc ggaaccgttc gtgatcaagg atatccgggt
     2401 ggagggcctg caacgcaccg aacccggcac cgtattcaac tatctgccgg tcaaggtcgg
     2461 cgataccttc tctgacgaca aggccgaatc cgccatcaag gcgctgttcg ccactggttt
     2521 ttttgccgat gtgcgcatcg aaaccgaaaa caatgtggtg attgtcgcca tcgccgagcg
     2581 tccggtgatt gccgagctga acatcaacgg ctcgaaagaa ttcgacagca agcagctgaa
     2641 aaaggccctg aaagacaacg gcctggctga gtcgcgcgta ttcgataaat cggtgctgga
     2701 acaagccgaa caagagctca agcgccagta ttacagcaag ggcaaatact cggtacagat
     2761 caacaccaag gtgaccaagc tggaacgcaa ccgcgtggcg gtcaccatgg acatcgacga
     2821 aggcgtggct gcgcgcatca agcagatcaa gatcgtcggc accaaggcct tcaaggaagg
     2881 cgatctcttg gacgaattcg ccctgaccga ctcgggctgg ctgacctggc tgacccgcga
     2941 cgaccagtat tccaagcaaa agctgcaagg cgacctggag cgtctgcgct cttactacct
     3001 gaaccgcggt tacttcgagt tcaatatcga ctccacccag gtgtcgatca gcccggacaa
     3061 gcgggatgtg tatatcaccg tcaacgtcag cgaaggcgaa aaatacactg tctcggacgt
     3121 gcgctttgcc ggcgatttga ccgtgccgga agccaccctc aagccgctgg tgcagatcaa
     3181 gccgggtgaa accttcaccc gcgacaaggt caacgacacc gtcagcgcca tcaccgacct
     3241 gctgggcaac agcggctatg cgtttgccaa cgtcaatgcc gtgccggaag tggacaagga
     3301 caaacatcag gtggcgttca ccttctttgt cgatccgggc cgccgtgtgt acgtgcgccg
     3361 catcaatgtg gccggcaaca cccgcacccg cgacgaagtg atccgccgcg aaatgcgcca
     3421 gatggaaagt ggctggtacg acaaccagaa aatcaagcgc agtaaagagc gtctggacct
     3481 gctgggctac ttcaccgacg tcaacgtgga aaccccggcc gtcaccgaca cgcctgacca
     3541 ggtggacgtg aatgtcagcg tcaccgaaaa acccaccggc agcgtgcagc tgggcgccgg
     3601 tttctcgcag gacaacggct ttgccctgtc cgcgtcgatc tcgcagacca acttctttgg
     3661 ttcgggcaag gccgtgtcgg cgggctttaa caccgacaag gtgaacgagt tctacaacct
     3721 gtcgttcacc gacccgtact ttaccgtcga tggcaccagc ctgggttatg acgtctaccg
     3781 caagacttac gaccccagcc agaaggatcg cggtcagtac aagaccaaaa ccgacggcat
     3841 catggtgcgt gccggtgtgc cgatcaccga attcgatcgc attaatttca gtgccggcgt
     3901 cgagcgcacc aagattggcc tgttctccga cagccccaag cgctatgttg acttcgtcaa
     3961 cgagtacagc agcagcaact tcagcctgct ggcttcggcc agctgggcgc gcgacacccg
     4021 cgactcggcg ctgtggccga ccaagggctc ggtaagcaaa gccgccgctg aatttgccct
     4081 gccgggcggt gatgtgcagt actaccgctt tagcgtaggc aatcagtggt actacccgct
     4141 gagcaaaaac ctgacgctga tgctgaatgg cgagctgggc atggtcaaca gctggtccag
     4201 caagaagctg ccgttctacg aaaacttcta cctgggcggc atcagctcgg tgcggggttt
     4261 tgaatccggc accattggcg cccaggatga aaacaacaac gcttacggcg gcacccgccg
     4321 tgcggtgttc aacgccgaag tgctgttccc cttcccgggg ctgcgcgacg acaaaaccct
     4381 gcgcctgtcc accttctttg acgtgggcac ggtgtttggc ggccagttca acagcggcct
     4441 gacctggcgc gataacctgc gctactcggc cggtctgggc ttgtcctggg tgtcgccgct
     4501 gggcccgatg cgcttcagct tctcgcagcc gctgcacaag aagtccaacg accatcttga
     4561 gcgcttccag ttcacgctgg gcacctcgtt ctaagcccat gatgagcaag cttgccttat
     4621 tgtggcgtgc gctgatggcc agtctggtgc tggccatgct ctggctggcc gcaccggcca
     4681 tggccgacga gctgcgcctg ggctacgtcg ataccgagcg ggtctatcgc gaggcaacgg
     4741 cggcggtgaa agcgcagcag cgcctgaaag ccgaattcgc cccgcgcgaa gctgagctgg
     4801 ccgacatggc gcgccgcgcg cgcgagctca agcaaacgct ggacgccagc aaactctccg
     4861 acgccgagcg gcgcacgcgc gagcgcgagc tgcaagcgct ggaccgcgac tttcaggcca
     4921 agcagctgga attccgccag gaattcggcc agcgccgcac cgaagaattt gccgccatcc
     4981 agcagcgcgc caaccaggtg atccgtgact tggcgcgcag cgaacgcttt gacctgattt
     5041 tgcaggatgt ggtgtacgtc agcccccgtt tcgacatcac cgagcgggtg ctgaaagcac
     5101 tggacgactg acgcgcgtca gcccgtctga cctggccgcc cgtgggcggc cgggcggcgt
     5161 ggctggcgcg tgtgtttttt tgcccgccaa ggaccataca tggcctggac cctttctgag
     5221 attgtcgccc gcctgggcgg cgaattgcac ggcgaagacc gcagcgtggc ccgcctggcg
     5281 ccgctggacg aagccggcgc cgacgagatt gcctttgtca ccggcgccaa gcacgccgac
     5341 gcgctgcgcg cctcggccgc tggcgcggtg attgtcaaag ccgacctgct ggacattgcc
     5401 gcccgcccgg cgattgtctg cgccgacccc tatgtgtatt ttgcccggct ggccgggctg
     5461 atgtgcccgc cggcggcggc gcgtgccgct ggcgcgcatc ccagcgcggc ggtggccgct
     5521 gacgcgcagg tggccgccag cgcggaaatc cgcgagtttg tcagcattgg cgccggcgcg
     5581 gtgatcggcg agcgggtgtt gttgatggcc ggcgcggtgg tcgaggccgg cgcggtgatc
     5641 ggcgacgatt gcgtgttgca cccgcgcgtg gtggtgcacc acggctgcgt ggtcggcgcc
     5701 cgctgcattc tgcatgctgg cgcggtgatt ggcgccgatg gctttggcaa cgcctgggcc
     5761 ggcgaccact gggaaaaaat cccgcaaatc ggccgcgcgg tgctgggcga cgatgtagaa
     5821 atcggcgcca acaccaccat cgaccgtggc gccctggccg acacggtgat tgagcgcggc
     5881 gtgcgcctgg ataatctgat ccacatcgcc cacaactgcc gggtgggcgc gcacaccgcc
     5941 atggccgcct gcgtcggcgt ggccggcagc accagcatcg gcgccaactg catgatcggc
     6001 ggcgcggcga tgatctccgg ccatctgaaa atcggcgacc gcgtgcacat ctccggcggc
     6061 acgctggtgg ccaaaaatct ggacaaacct ggccagtaca ccgccgtgta cccgctggcc
     6121 acccataaag agtggctggg caacgccgcg catgtgcgtc agctgggcaa gctggcggcg
     6181 cgcgtcaagc agctggaagc ccgtctggct gccgccaacg ccaccgaccc ccactccacc
     6241 aaggaacccc aatgagcgaa tttgccaccc tgatcgacct gcgcgccatt ctcaagctgc
     6301 tgccgcaccg ctacccgttt ttgctggtgg accgcgtcac cgaactggaa gtcggcaagc
     6361 gcatcgtcgc cctgaaaaac gtcaccatca acgagccgtt ttttgtcggc cactttgacg
     6421 gctacccggt gatgccgggc gtgctgattg tcgaagcgct ggcccaggcc gccggcatcc
     6481 tggcggtgaa aacccacgat ggcgatgtgc gtcaggaaaa cgccctgtat ttctttgtcg
     6541 gcatcgacaa ctgccgcttc aagcgccagg tgatccccgg cgaccagctg accctggaag
     6601 tggaaatgct gcgcgtggcg cgcggcatcg gcaaattccg cgccgtggcc aaggtggatg
     6661 gcgaaatcgc cgccgaagcg gatctgatgt gcgcgcgccg ggaggtgtaa gccatggcga
     6721 ttcatcccac cgccattatt gcgccgggcg cacagattga cgccagcgtg gagattggcc
     6781 cttacgcggt gattggcgag catgtcagca tcggcgccgg cacccgcgtg ggcccgcaca
     6841 cggtgattga aggccacacc accattggcc gccagaacca gatttttcag ttcgcctcgc
     6901 tgggcgccgc gccgcaagac aaaaaatacg ccggcgaacc cacccggctg gaaattggcg
     6961 acaacaacac catccgcgag ttttgcacct ttaacgtcgg caccgtgcaa gacggcggcc
     7021 tgacccggct gggcaacgac aactgggtga tggcctatgt gcacatcgcc catgattgcc
     7081 tggtgggtga ccactgcatc ctggccaata acgccaccct ggccggccac atcagcatcg
     7141 gcgaccatgt gtttctcggc gggctgaccg ccgtgcatca gttttgcacc atcggcgccc
     7201 acgccatgac cgccggcggc tcgattgtcg tgcaggacat cccgccctat gtcaccgccg
     7261 ccggcaacca cgcccacccg gccggcatca atagcgaagg cctgcgccgc cgtggctttg
     7321 acgccgagca aatcagccgc atcaaacgcg cctacaagca gatttaccgc cagggcctga
     7381 gcctggaaga cgccaaggcc gccatcaccg aggccgccgc cgacgccccg gaactggcgc
     7441 tgtttgtgga cttttttgcc cgcgccaagc gcggcatcat ccgctgatgg gcctgtttcc
     7501 acccgacgcc ggcctgcgca tcgccatggt ggcaggcgaa gcctcgggcg atatgctggg
     7561 cgcgcggctg atcgacgcca tccgcgcccg ccgcccggat gcgcgctttg tcggcatcgg
     7621 cggccccaaa atgcaggccg ccgggctgga aagcctgttt ccgcaagaaa ccctggccgt
     7681 gcgcggctat gtggaagtgc tgcgcaatct gcgcgccatc ctcgccatcc gccgcgagct
     7741 caaggccaag ctgctggccg agcgcccgga cgtgttcatc ggcgtggacg cgccggattt
     7801 caatctggcg gttgaacaca gcctgaaagc cgccggcatt cgcaccattc actacgtcag
     7861 cccgtcgatc tgggcctggc gcggcgagcg cgtgcacaaa atcaaacaag cggtaaaccg
     7921 cgtgctggcg ctgtttccga tggagccgcc gatttaccag gcggccggcg tgccggtcag
     7981 ctatgtgggc cacccgctgg cggaaagcat gcccatcgtc cccgaccgcc agggcgcgcg
     8041 tgaggtgctg aacatcctgc cgcgccaggc gccagtgttc accctgatgc ccggcagccg
     8101 ccagggcgaa gtggacgtga tggccccgct gttcatcgcc gccgcccgct tgatcgccaa
     8161 agaagtgcca gatgcgcaat ttttggtgcc gttggccacc cgcccgacct ttgaccgctt
     8221 tgaacaactg ctgtggcggc tgaaagccca ccaactgccc atccgccgcc tgttcggcca
     8281 cgcccaacac gccatggctg ccgccgacgc cgtgctggtg gccagcggca ccgccacact
     8341 ggaagtggcc ctggccaaac ggccgatggt gatcagctac aaaatctcgc cgatcaccta
     8401 ccgcatcgtc aaacgcaaac tgcaactgcc ctacgtcggc ctgcccaata tcctgtgcgg
     8461 cgaattcatc gtgccggaac tgctgcaaga cgacgccacc ccggaaaacc tcgcccaggc
     8521 cctgctcaac gcctggcgcg acgccgacta ccgcgcctgg ctggatggca aactgaccga
     8581 actgcaccac acgctcaagc aggatagcgc tgcacgtgcg gcacaagcgg tgctggaggt
     8641 ggcagaacat ggctgaacaa cagaaccgac tctgcggcgt tgacgaagcc ggccgtggcc
     8701 ccttggccgg tgcggtgttt gccgcggcgg tgattctcga ccccacccgc ccgatcagcg
     8761 gcctggccga ttccaaaaaa ctctccgaag ccaggcgcga tgcgctggcg ctggaaatcc
     8821 gcgaaaaagc cctggcctgg gcgattgcca gcgccagtgt ggaagaaatc gaccagatca
     8881 atatcctgca cgccagcatg ctggccatgg cgcgggcggt ggatgcgctg gcagtgacgc
     8941 ccagcgaagt gcaggtggat ggcaaccgca cgcccaagct gttcaaaccg ctgccggtca
     9001 gcgccatcgt caaaggcgac gccaaggtgg cggagatttc cgcggcgtcg attctggcca
     9061 aaactgcgcg cgacgccgag ctggtggcgc tggatgcgca atatccggcc tatggctttg
     9121 cccgccacaa gggctacccc accgccgagc atctggcagc gattgccgaa cacggcgtgc
     9181 tgagcattca ccgcaagaca tttggcccgg tcaaagcctg gctggcgacg catcagggcg
     9241 agttgttctg atggcggcgg tgggcgaggg cgctgcgctg gcgcgctgtt ttgccccggt
     9301 ggtcaacgcc gacacgcagg tgttgattct gggcagcctg cccggcgcgg cctcgctggc
     9361 ggcgcagcag tattacgccc acccgcgcaa ccagttctgg ccgctgctgg cggcggtgct
     9421 gaagcagccc gacttgccgg cgctggccta ccccgcgcgg ctgcaagcct tgctggcggc
     9481 gggggtcggg ctgtgggatg tggtggcgca ggcgcaacgg cgcggcagcc tggacgccaa
     9541 tattcgcgat gcccaggcca acccgctgcc ggcgctgatt gcccagctgc cgcgcctgca
     9601 cacggtggcc ttcaatggcg ccaccgccgc gcgggcggcg gtgcaactga cgccttaccc
     9661 ctggctgcgg gtattgccgc tgccctcgtc cagtccggcg cacacgctgg cgtttgccga
     9721 caagctggcc gcctggcagg ccctgggcga cgacatacac tagcctgtca ctgcttgagc
     9781 atgtctttgc ccagcccggc ctgtcggtga cagcgccaga cccggtgctt acatcacatc
     9841 cacccggaag ctctcgccgc aactggaaga aaacccttcc ttggcggctt gcaacagcgg
     9901 ccccagcttg ggctgctgac gcgggatgct gtccggcagg ttcatgtaga tgtctaccag
     9961 ttggccattg gtgcagaaga atttcatccg cttgtgcgca ttggcgccca ggccctgatc
    10021 aatgacggca aacagatcct gggtgggaat gctcaggccc cggttggccg caatgaacgg
    10081 cccgacaccc gaggcattga attgcttgac cagatcaatg gccacctcaa agtagccatc
    10141 ggcatcccat tcagtctggc aggtgccgtg cttgaaccac tcatgacgtt gcaggcaaga
    10201 acccgccgca gcgctgggca tcacttcgcc cagttggcgc atggtgctgg cgctgatggc
    10261 cggctcagga tagtcgcaga aattcttctg ctgcggctgg ctgctgcaat agccataatt
    10321 ggtgccgcag cgccccagat tgggccacag gccatgcagg gtgaagttct ggctggccca
    10381 cggggtgggc tgctgacatt ctggcttgct ttgatgggtt tcacagaaag ccgcctgcca
    10441 gctgacggca aacacatagc tgtcaccttg cccggccaga ttgcaggcct tgccgctgct
    10501 ggctgacgac gtgctgctgg tgctggcagg tctggatgtc agcgcacccc gaccgcaatg
    10561 ggccgatacc cagcgctctg ccggctgtgc gccttgggct tgtatccgat accaggtcgg
    10621 ggcattgggt gcatcagcgt caatgatcgg atacaccacc ccggactgca cggtgaactg
    10681 atcgggatta ttgtcaggct tgccggcctt gggcttgttg acggtggcgg tgcaggcttg
    10741 ctcagcagag aatgcgccag catagccggc agcgtgggcc tgggcggtca gaccgccgag
    10801 caccagggcc agcgtcaggg caagagaaga gcgtgacaga atcatgcagg gctccttggg
    10861 aagggaagat gaacgggcgg cttgccatat ccacaacggc aatttatgca ttattcatca
    10921 tacccattca tgtctgacaa taactgacta cccccttctc cccgttacca cttgccttcc
    10981 agcatcagca aaaagctgcg gttggcgcga cggctttcca cttcctggcg cttgagcgca
    11041 ccagtgctgt cgtactccca cacctgattg cgggcttttt caccggtaac atgctgtacc
    11101 gaggcgcgtg gagggcagga gaaaaaagcc gtgagcatcc gaaggctttg ttaagagccg
    11161 ctcacaaaac tcagcgaagc gcgcctggct aggtgcgcag ccgcagacag tacaaggcgt
    11221 acggccaggc tgcgcaacaa cgccaggagg gtcttgttag cagctctaac cacactcccc
    11281 ccgccgccag tcgctcgggc tgcaccccat ggcctggcgg aacatatggc tgaacgccga
    11341 gggtgaggcg tagcccagct ccagcgccac gtcggtgacg gcgcggccgg cgcccagcca
    11401 ttccagcgcg cggatcagcc gcaggcgggt gcgccagtcg cgcaggctga tgcccagttc
    11461 gcgctcgaag cgtcggctca gcgtgcgcgc cgaggcgccc agcgcggcgc cccaggcggg
    11521 caggtcgcgc gggtcggcgg gctgggcgta cagcgcctcg cacagggtgc gcagctgcgg
    11581 gtcttttggc cacggcaggt gcacatcctg ccggggcagg cgcggcagtt gatcgacaat
    11641 cagctgctgc acgcgggcgg tgtagtcggc gctttcctgg cgctggccaa tggcgtccag
    11701 ctcgacaatc agcgcgcgca gcagcggggt ggcggccagc acctggcagt gctcgggcat
    11761 gtccagcgcc agcgcggcgt ccacatacaa attgcgaaat tccgcatcgt gcagggcgcc
    11821 ggtggcgtgc agcacgccag tgggcagcca caccgcttgc tcgggggtga tcacgcacca
    11881 ggtgtctggc gcctgcacca gcagcgcgcc ggcagtggcg tacaccagct gacgccagcg
    11941 gtgggtgtgc ggcaaaaaac tgtgccgcgc cggcaggcgc tgcgagcgca aaaacaccgg
    12001 gctgggcagg gtgtcgattt ctggcaggtc tacggcgcgc cagtctgggc caaggggcat
    12061 ggcagctgtc ttctcatcga tagtgatcgt cagattatcg aatgccggcc agccgcgcca
    12121 gccttaccat ggcgcattgc ctgccggagc gcccatcatg tctattgctg tccccctcga
    12181 ccccgccctg gccagcgccc gctggcgggt gcttgccgct ggcgtgtgcg cgctgattct
    12241 caccgtgggg ctggcgcgct ttgcctatac gccgatgctg ccgatcatgc gcgcgcaagc
    12301 cgggctgagc gatctggccg gcggctggct ggccacgttc aattatgtcg gctatatcag
    12361 cggcgccttg ctggcggcaa ccttgtcgcg cttgcaggat aaattccgcc tgtatcggct
    12421 ggggctggcg ctggcggtgc tgaccacgct gggcatgggg ctgaccgaca atctgtatct
    12481 gtggacggcc ttgcgcctgc tggccgggat ttccagcacc gccggcttgc tgattgcctc
    12541 tggcctggtg ctgaactggc tgattcgcca cggctgtcgc gccgagctgg gcctgcactt
    12601 taccggcatt ggcctgggca ttgtggtgtc cgggctggcg gtggcggcga tggcgtcgct
    12661 ggattgggcc gggcagtggc tgggcctggg cgtgctgggc ctggttttca tgctgcccgc
    12721 ctggtggtgg atgcccgcgc cagccggggc cggcgccacc gccacagcgc cggccgccgg
    12781 gcccagccgg cgctggatgg ggttttttgt ggcggcgtat ttttgcgcgg ggtttggcta
    12841 tgtgatcagc gcgaccttta tcatcgccat tgtcggcaag ctgccgctgc tggccaatct
    12901 gggcggctgg gtgtgggtgc tggtggggct gatggccacg ccgtcgtgct ttgtctggga
    12961 ccgattggca gcgcggctgg ggcagattcc ggcgcttttg ctggcctatg gcctgcaaac
    13021 cgtgtcgatt gccctgccgg cggccagtga gggcgcgctg ctgaacctgc tgagcgcggc
    13081 cttgtacggc gggacctttg tcgggattgt cagcctgacg ctgtcgatta ttggccggca
    13141 ttttccggcc aacccggcca aggcgatggc gcgcatgacg ctcagctatg gcgtggcgca
    13201 gattgtggcg ccggcgctgg ctggctatat cgccaccgtc accggcagct atcgcggcgc
    13261 gctgtggctg gcggcggcgg tgatgctggc cggcatggcg gcgctggcgg ccctgcggga
    13321 aaaacccgct caccgccctt gacaaaatca acaagcccgc agtacattgc cacccacagc
    13381 gccgatttga gcttcagctt gcgggcgctt tacaaatgca gctaaagcgt atgcgaaacc
    13441 cgcacagctt tggctggcgg gtttttcgtt ttggccggcc cgcccggcag cgccgattta
    13501 atgcacacaa cttgcaggcg cttgataaat cttgctaaag cgtggcccac acgggcttcg
    13561 cacaccgaac aggagatgcc cgatgtacga ttgccaatcc gacagctacg ccgatttcac
    13621 cgtcgcccag cagaccacca cgaccaccac gcaggcgctg cgccgccccg agccactggc
    13681 cggcgatgtg cgcgtgcgtg ccctgcccgg ccacgaggcc gtgctcaccg agctggccat
    13741 cagcgccgag cgccagggct atctggccga gcgacgcggc gcacggctga cgctgtttgc
    13801 ggtgacgctg gctgacgtgc tggcattcaa ccgtgccttt gccacccgcg ccaccttcag
    13861 cgccgaattc cgcctggcag cgccagtggc gtttcatggc tgagcggttc gcccacgact
    13921 gaaaaaaccc aggattgccg ctggtgcgga catcctgggt tttgattttt tggcaaa
//
LOCUS       DAOPYX010000038        13966 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_4773_length_13966_cov_7.003810, whole genome shotgun sequence.
ACCESSION   DAOPYX010000038 DAOPYX010000000
VERSION     DAOPYX010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 13966)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13966)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13966
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4773_length_13966_cov_7.003810"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(51..2066)
                     /locus_tag="PKN55_03350"
     CDS             complement(51..2066)
                     /locus_tag="PKN55_03350"
                     /inference="COORDINATES: protein motif:HMM:NF020084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNX70314.1"
                     /translation="MLFFRKKAVDDGAPASGARADALPPVQVIRAQAGQLAQALPAQP
                     EPRVRLALGYVSPNANMDAVARSVRERFPRAAVVLTSTAGELCATRAGDALYQDTPQG
                     WQGVVLQLFSEAIIEQAHVANVPLGCEDIRQGAPKLRHDARVAQLARTLGQLSVPFSM
                     RAEEGFVLTLVDGLSNSESFLMEAVYESNRFPYLFVGGSAGGLLDFSHTRLNDGQQTR
                     ENHAMLCFIKLTPAYRYGVFKTQNFEDTGKSFLIAGANTELRTVDSVLEQKTGEVRDM
                     IGELCQHFHCREDELDGKLQNHTFGIKINGEIFVRSVLQINRQTRKLHFACDIAFGET
                     LHLLKAVDFASKTANDYRTFSQNKPAPVGGILSDCILRRLNNAAQLARAQVYGDVPVA
                     GFSTFGELLGVNINQTLTAVFFYPASQGFRDEFVDQFTLHYANYKNYFFRRELSRSTV
                     ISGMKSQIIQDLQGYKTLATELGETLPAFRGNAQTQIEQLESIETRLRDFSTHVSDGA
                     RSTESVRQQIGELAEHAGQIGNVLGMIKKIAEQTNLLALNAAIEAARAGEAGRGFAVV
                     ADEVRKLANNTQSNLETTGGSIDNVLRSIEQVSSQIHQVGELVGGFAERMGDTLDTLQ
                     QLAGAARQGKGDVEAMLAHTESLYQHMQKVDQEMAGILLLERSDLAQ"
     gene            complement(2308..2868)
                     /locus_tag="PKN55_03355"
     CDS             complement(2308..2868)
                     /locus_tag="PKN55_03355"
                     /inference="COORDINATES: protein motif:HMM:NF013786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HNX70315.1"
                     /translation="MDAAGLSAPRWLRRAEHAPAQLAAGETLLLPVRTAGLAPALARQ
                     WLRAQLDALPCLATGWRDSPRGPQPHNPALRLSLSYGQGVAVLALRAGAVGVDLTPLT
                     PPVDWPAVAATYFSPAHCAQLAALPPAQQGAAFAAAWAALEAGNKCLGLALTEYCADS
                     ARQRAALTIWHDRSQLTGHMLALAWS"
     gene            complement(2850..6824)
                     /locus_tag="PKN55_03360"
     CDS             complement(2850..6824)
                     /locus_tag="PKN55_03360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019922399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HNX70316.1"
                     /translation="MILHGLSRPDFLTDTGLPDLLEATAARQPDRPALYWGELCLSYG
                     ELLARASLAAHHLLQAGAAPGQHIGLWMPRGAEALVMQAAITLTGAAWLPFDADTPPE
                     RVEICLRDAGALGLVCHPDMAARLSDVNLPRWTPEALQAAVDGPLRRREGLRPEHPAY
                     VIYTSGSTGVPKGIAVSHGSICHFLRSENSVLGVREDDLVYQGFSLAFDMSFEEIWIS
                     YLVGAALWIAPKCLVSDPDALPEALIRQRVTVLHAVPSLLALFGRDVPNLRIINLGGE
                     MCPQALVERWATHERPLFNTYGPTEATVSASLARLVPGQPVTIGQPLPNYGLLVVDPA
                     DMRLLPAGEVGELCIVGPGVASGYLGRPELTAEKFLDNPFIERGYDRRLYRTGDLAKI
                     DETGQVQCLGRADDQVKIRGFRVELGEIEAALCDQADIGAAAVIVKAIDEVDQLIAYL
                     VTDSGQPANVAALRAGLRERLPPYMVPALFITLDAMPRLTSGKVDRKALRARELDALP
                     AVEDQAEPTNDAERELFGHLRRLFPGQPLAREADFFNDLGGHSLLAARLVSALRQTPR
                     YAGITVQEIYRARTVGAMASALEQLSAQAAANVAPSFAAVSPLKRALCGLAQAAMLPP
                     LVSLRMLQWLAPFFTYHFLTGDPDDSIALAVAASLLVFLLAHVASFAVAIAGKWLVLG
                     RLKPGRYPLWGVTYFRWWLADRLADVPATYLLHGSPLLTAYWRAMGARIGQDTLIGSM
                     SLRAPDAVSIGAGVNIGASVNLENVRLEPDALVVGQIEIGDAAYVGSYGVLEGGSRIG
                     AGAYLDGLSSLAQGQQVPAGERWEGAPARRSAQPIRPRPTPPARPGLPMRALALAGYV
                     LGAAAVAALFFLPVFPSFILIDWLDVHELDLGQLGLPAWQAFFWYALLALPASAVLVA
                     ATILLSAAIRWLVLPRLQPGRWSVFSRVYYRKWLTNQIQESSLHVLHGLYATVFAPFW
                     YRVLGARIGKGAEISTAMGVVPDMLTLGDDTFIADAVMLGDEEIDSGWMTLRPTEIGA
                     RSFVGNGAYLPDGTELPADVLIGVQSRAPANGQMRAGDTWMGSPALHLPAREVTSGFA
                     EHLTFRPSVARRIGRGTVEAVRIVLPLALIIASGYLTVIKVLPAAETEHWALAAWRLC
                     LAGLCYGVGSFAVVLAAKWLLIGRYTPRAAPMWTPFVWLSEAVTNLYESIAVPNFVNF
                     LRGTPFLPWVLRLLGCQIGRGVVLDTTDVTEYDCVRIGDGSVLNAWSGPQTHLFEDRV
                     MKIGRVDIGAQVLVGARTTVLYDSQVGDGALLGPLSLVMKGEHIPPRTQWNGSPCKPW
                     TPPA"
     gene            complement(6784..>6989)
                     /locus_tag="PKN55_03365"
     CDS             complement(6784..>6989)
                     /locus_tag="PKN55_03365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX70317.1"
                     /translation="ERRLTWLYTARQPNDRPGVIPETGSHRKVLLPILAIADHTRQAV
                     ACREPWIRLPDDFARTFPPGFFD"
     assembly_gap    6990..7089
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7090..8113)
                     /locus_tag="PKN55_03370"
     CDS             complement(<7090..8113)
                     /locus_tag="PKN55_03370"
                     /inference="COORDINATES: protein motif:HMM:NF019040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HNX70318.1"
                     /translation="MFVLLRGGLDGLHAISPVADPRWPSLRPTLAQPVLQSGLALPGS
                     GFALHPALADCLTLYRAGELWFCPTAGSPNTSRSHFQAQDVMELGTGAISGNTGWMGR
                     LASQLDGRLAANFAANAPLALYGAGVAIEHIPLARQALRLPGGALQEALLRAHAGSPS
                     QRWLQQAIDTQALLAQADPDQASRNAAPLAGWAAQARQMGQVLRQQPELALAFVDLSG
                     VDSHVNQAAGLAQPLTQLGQGLLAMKTALGEAEWARTRVVVLSEFGRTAAENGTRGTD
                     HGHGGLALLAGGGLGREGRMLGDFAGLQANALFEGRDLPVTLDWRSLLAAVSQAVFGL
                     SARALDQ"
     gene            8408..8767
                     /locus_tag="PKN55_03375"
     CDS             8408..8767
                     /locus_tag="PKN55_03375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008953285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HNX70319.1"
                     /translation="MVETFLVLIGLQLLGEWLARWLHIPVPGPVLGMLLLFVGLCLRR
                     GVPTALQHGVPAFLQHLSLLFVPAGAGVLLYAHLLNGQTVWQLALALAVGTTVTLLVS
                     ALLLAGLMRWRGEHGHD"
     gene            8760..9476
                     /locus_tag="PKN55_03380"
     CDS             8760..9476
                     /locus_tag="PKN55_03380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011137153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HNX70320.1"
                     /translation="MTDFASLWQAARQQPLTWVFLTMLCYRLAIALNRQSGGHALANP
                     VGLGALLLIGVLLATGTDYKQYFEGGKFIQMLLGPATVALAVPLYANLHRLSKVFWPL
                     LISLLLGSLVGIVSSAGVAWALGLDDILIKSLLSRSVSTPISMGIASEIGGAPELAAV
                     FVIVSGVFGAMLGWPLLSRLRLGQDVVYGFATGMAAHGIGTARAFQRSDTAGAFSGLA
                     MGLNGLVTAILIPLLLRFWH"
     gene            9591..>9988
                     /locus_tag="PKN55_03385"
     CDS             9591..>9988
                     /locus_tag="PKN55_03385"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HNX70321.1"
                     /translation="MDLILWRHAEAEEGSNDLARELTRKGQQQAGLMAAWLRLRLPRD
                     FQLLASEARRSQQTAAFLSKNYRVLPEINPDATVESVLKASHWPDARHPIVLVGHQPY
                     MGMIASQLIGGEAQMWNVKKGGVWWISQRVR"
     assembly_gap    9989..10088
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<10089..11595)
                     /locus_tag="PKN55_03390"
     CDS             complement(<10089..11595)
                     /locus_tag="PKN55_03390"
                     /inference="COORDINATES: protein motif:HMM:NF020390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 domain-containing protein"
                     /protein_id="HNX70322.1"
                     /translation="MKQAMRVLGGVACMLATLTQAAGPLKLDTPMDAPLARAMLARWG
                     YGATDASVAALTGQTPRAMVQRGWRAPSGVAPAVRTALDALADAPPLAERWAQYGPVR
                     VQEARKAQDIPLVQSMARQGNAMKSATVQRRLLMMANSDNPAHEVLLAFWLNHFSVYL
                     HKGQLPWLVDDYASRIEAAMADDRFESLLRAAFYHTAMQGYLDNINSTAPDSELGRRG
                     RTGLNENLARELLELHTLGVGGGYQQADVEATARLISGVSAPDLGTADALADGGFALD
                     PRRRGEGSVQVLGQTYALADGRAAMDALLHRLALHPATARHVSRKLAQRFVGDTPPAA
                     LVERMAQAYVQSGGRLSPALWVMLASPAFADSLLKQRMFRLPQDYVLATARAVCQDAP
                     ITHAEPLLNMLQAMGQAPFLRLTPDGYPQNAGEWQTALGMALRVQLAQALAQRQWRLT
                     GPADAAQACNWDAAAAERSLGPWTAATRAAAEGLPPAQQAILLLSSPATLWR"
     gene            11741..12490
                     /gene="pgeF"
                     /locus_tag="PKN55_03395"
     CDS             11741..12490
                     /gene="pgeF"
                     /locus_tag="PKN55_03395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003780787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HNX70323.1"
                     /translation="MSADFFLRPHWAAPAHVHALSTTRQGGVSQPPYASFNLGAHVGD
                     DPAAVAANRALLRQQLPAEPAWLNQVHGIHVVDAASVAGTPVDADASFSRTPGVVCAV
                     MTADCLPVLFTDRAGSVVAAAHAGWRGLLAGVLEATLAAMACPAHEVLAWCGPAISRD
                     AFEVGSEVRAAFVAHNPAAAAAFSPLPDGKWLADLPGLARQRLLAAGVPDNQCVDSEL
                     CTVIEREQFFSYRRDGCTGRMASLIWLAPAV"
     gene            12538..13254
                     /locus_tag="PKN55_03400"
     CDS             12538..13254
                     /locus_tag="PKN55_03400"
                     /inference="COORDINATES: protein motif:HMM:NF005436.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNX70324.1"
                     /translation="MIKAIVSGHSRGLGAAIVEALLARQLPVLGLARSALPARAGLSQ
                     CALDLADPAALTAWLAGPTLHDWLADASAVWLVNNAGQVQPIAALGEQDPQAIVQAIN
                     LNVTAPLLLSNAVLAASPHCVERRLLHVSSGAARHAYPGWSVYCASKAALDHHAQAVA
                     AERHTGVRLVSLAPGVIDTEMQADIRAASLAQFPLRERFDALKANGQLTSPQTCAEHL
                     VAALAHPAFGEVVLTDLRHG"
     gene            13358..>13966
                     /locus_tag="PKN55_03405"
     CDS             13358..>13966
                     /locus_tag="PKN55_03405"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF012630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031012 - extracellular matrix [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="matrixin family metalloprotease"
                     /protein_id="HNX70325.1"
                     /translation="MTAPTSSASFAVIGSSSSATTNALISGFKWGSSTVGSSASLSYS
                     FPWASGGSATFAGLNGQSYSDLNEQNTGTALNTTQQAAFRSALQAWANVANLSFSEVS
                     DTRSNVGDIRVAWTSAAYGISTGEQAWGWAGYPGAWPSAGDVWISARGSDTASQSWAA
                     GSYNFEALMHELGHALGLKHPFEDSPTLAKSLDTRQYTLMSYT"
BASE COUNT         2429 a         4696 c         4346 g         2295 t
ORIGIN      
        1 cgctttgagc ggctcacacc atgaaggccc ccggctttgc cgggggattg ttactgcgcc
       61 aggtcactgc gttccagcag cagaatgccc gccatttctt gatctacttt ctgcatatgc
      121 tgatacagcg attcggtatg cgcgagcatc gcctcgacgt cgcccttgcc ttgccgtgca
      181 gcgccggcca gctgttgcag ggtgtccagc gtatcgccca tgcgctcggc aaagccaccc
      241 accagctcgc ccacctggtg aatctggctg ctgacctgct cgattgagcg caagacattg
      301 tcgatcgagc cgccagtggt ttccagattg gattgggtgt tgttggccag cttgcgcact
      361 tcgtcggcca ccacggcaaa acctcggccg gcttcgccgg cgcgcgcggc ctcaatggcg
      421 gcgttcaagg ccagcagatt ggtctgctcg gcgattttct tgatcatgcc cagcacattg
      481 ccaatttgcc cggcgtgctc ggccagctcg ccaatttgct ggcgcaccga ttcggtcgag
      541 cgggcgccgt cgctgacatg ggtgctgaaa tcgcgcagcc gggtttcgat gctttccagt
      601 tgctcaatct gcgtctgcgc attgccacgg aaggccggca gggtttcgcc cagctcggtg
      661 gccagggttt tgtagccctg caaatcctgg atgatctggc ttttcatgcc gctgatcacc
      721 gtgctgcgcg acagctcgcg gcggaaaaag taatttttgt aattggcgta atgcagggtg
      781 aactgatcga caaactcatc gcggaaaccc tggctggccg ggtagaaaaa caccgccgtc
      841 agggtctggt tgatattcac ccccaagagc tcgccaaaag tggaaaaccc ggccaccggc
      901 acatcgccat acacctgggc gcgagcgagc tgggcagcgt tgttcagccg gcgcaaaatg
      961 cagtcggaca aaatcccgcc cactggcgcc ggtttgttct ggctgaaggt acggtagtca
     1021 ttggcggtct tgctggcaaa atccaccgcc ttgagcaggt gcagggtttc accaaaggcg
     1081 atgtcacagg caaagtgcag cttgcgcgtt tgccggttga tttgcagcac cgagcgcaca
     1141 aagatttcgc cattgatctt gatgccgaaa gtatggtttt gcagcttgcc gtccagctcg
     1201 tcctcgcggc aatgaaaatg ctggcacagc tcgccgatca tgtcgcgcac ttcgccggtt
     1261 ttttgctcca gcactgaatc caccgtgcgc agctcggtat tggcgccggc aatcaggaaa
     1321 gatttgcctg tgtcttcaaa gttctgggtt ttgaacacgc catagcgata cgccggggtg
     1381 agcttgataa agcacagcat ggcgtggttt tcgcgggttt gctggccgtc attcagccgg
     1441 gtgtggctga agtccagcaa accgccggcc gagccgccaa caaacagata cggaaagcgg
     1501 ttggattcgt acaccgcttc catcaaaaaa ctctcgctat tggacagccc atccaccagg
     1561 gtcagcacaa agccttcttc cgcgcgcatc gagaacggca ccgacaattg gcccagcgtg
     1621 cgcgccagct gcgccacccg cgcatcgtgg cgcagcttgg gtgcaccctg gcggatgtcc
     1681 tcacagccca gcggcacatt ggccacatgc gcctgctcga tgatggcctc gctgaacagc
     1741 tgcaacacca cgccctgcca gccttgcggg gtgtcctggt acagcgcatc gcccgcccgc
     1801 gtggcgcaca gctcgcccgc cgtgctggtc agcaccacgg ccgcgcgcgg aaagcgctcg
     1861 cgcacgctgc gcgccacggc gtccatattg gcgttggggc tgacatagcc caatgccagc
     1921 cgcacgcgcg gctcgggctg ggcaggcagc gcctgcgcca attgcccggc ctgggcacgg
     1981 atcacttgca cggggggaag ggcgtcggcg cgcgcgccag acgcgggcgc accgtcgtca
     2041 acggcttttt tgcggaagaa caacatctcg gcctcacttg gtgggtggtg tgtatgccgg
     2101 cgcttcatgg tggcgccgtg catcggccgg acgggccggc ctggcccgga caagagggcc
     2161 gccgggccgg aaagcatacg cagcatgcca gcagaagcag atggcataca tattgacgct
     2221 aggatacaac tgtttgaaca agtgtgcagc gccgatgccg cgcttgccat tagacaaaag
     2281 tgctgaaacc caagtagata caatatgtca tgaccaggcc agcgccagca tatggccagt
     2341 caactggctg cggtcgtgcc atatggtcaa cgccgcgcgc tggcgtgcgc tgtcggcgca
     2401 gtattccgtc agcgccagcc ccaggcattt attacccgcc tccagcgccg cccaggcagc
     2461 ggcaaaagcc gcaccctgct gcgcgggcgg taaagcggcc agttgcgcgc agtgcgccgg
     2521 gctgaaatac gtcgctgcca ccgccggcca gtctactggc ggcgtcagtg gggtgagatc
     2581 cacgccgacc gcacccgcgc gcagcgccag caccgccacg ccctggccat aactcaaact
     2641 cagccgcaag gccgggttgt gcggctgcgg cccgcgtggg ctgtcgcgcc agccggtggc
     2701 cagacagggc agcgcgtcca gttgcgcgcg cagccattgc cgcgccaagg ccggcgccag
     2761 gccggcggtg cgcaccggca gcagcagcgt ttcgccagcg gccagctgcg ccggggcgtg
     2821 ctcggcgcgg cgcagccagc gcggcgcgct caggccggcg gcgtccatgg cttacagggc
     2881 gagccgttcc attgagtgcg cggcgggatg tgctcgccct tcatcaccag cgacaacggc
     2941 cccagcagcg cgccatcgcc cacctggctg tcgtacaaca ccgtggtgcg cgcgcccacc
     3001 agcacctgcg cgccaatgtc cacccggccg attttcatca cccggtcttc aaacaaatgc
     3061 gtctgcgggc cagaccaggc attcagcacg ctgccatcgc caatgcgcac gcagtcgtat
     3121 tcggtcacgt cggtggtgtc cagcaccacg ccacggccga tctgacaacc cagcaggcgc
     3181 agcacccacg gcaaaaatgg cgtgccgcgc aaaaagttga caaagttcgg cacggcgatg
     3241 gattcataca gattggtcac cgcctcgctc agccagacaa atggcgtcca catcggcgcg
     3301 gcacgcgggg tgtagcggcc aatcagcagc catttggctg ccagcaccac ggcaaacgag
     3361 cccacgccgt agcacaagcc cgccagacac agccgccagg cggccagcgc ccagtgctcg
     3421 gtttcagcgg cgggcagcac cttgatcacc gtcagatacc cgctggcgat gatcaaggcc
     3481 aacggtagca caatgcgtac cgcttctacc gtgccacggc cgatgcgtcg ggccaccgag
     3541 gggcggaagg tcaggtgttc ggcaaaaccg ctggtgactt cccgcgccgg caggtgcagc
     3601 gcaggcgagc ccatccaggt gtcgccggct cgcatctggc cattggctgg cgcacgcgat
     3661 tgcacgccaa tcagcacatc agccggcaac tcggtgccat cgggcaggta ggcgccattg
     3721 ccgacaaagc tgcgtgcgcc gatttcggtg gggcgcagtg tcatccagcc gctgtcgatt
     3781 tcttcgtcgc ccagcatcac tgcgtcagca ataaaggtgt cgtcgcccag cgtcagcatg
     3841 tccggcacca cgcccatggc ggtggaaatc tcggcgccct tgccaatgcg tgcgcccagc
     3901 acccggtacc agaatggcgc aaacaccgtg gcgtacaggc catgcagcac atgcaggctg
     3961 gattcctgaa tctggttggt cagccatttg cggtaataca cccggctgaa taccgaccag
     4021 cgccccggtt gcagacgcgg cagcaccagc cagcggatgg ccgccgacag caaaatggtg
     4081 gccgccacca gcaccgcgct ggccggcaag gccagcaagg cataccagaa aaacgcctgc
     4141 caggccggca agcccagttg gcccaggtcc agctcgtgca cgtccagcca gtcaatcagg
     4201 ataaagctgg ggaacaccgg caagaagaac agcgccgcca ccgccgccgc gcccagcaca
     4261 tagccggcca gtgccaacgc ccgcatcggc aggccggggc gcgctggcgg ggtgggccgt
     4321 gggcggatgg gttgggcgct gcgccgtgcc ggcgcgccct cccagcgctc gcccgccggc
     4381 acctgctggc cctgcgccag gctggacagc ccatccagat acgcgccagc accaatgcgg
     4441 ctaccgccct ccagcacacc atacgagccg acataggccg cgtcgccaat ctcaatctgc
     4501 cccaccacca gcgcatccgg ctccaggcgc acgttttcca ggttcaccga agcgccgata
     4561 ttcacgcccg caccaatgct caccgcatct ggcgcgcgca gcgacatcga gccaatcagg
     4621 gtatcttggc caatccgtgc acccatcgcc cgccaatagg cggtgagcag cggcgagccg
     4681 tgcagcagat aggtggccgg cacatcagcc agccggtcgg ccagccacca gcggaaatac
     4741 gttacgcccc acagcgggta gcgaccgggc ttgagtcgcc caagcaccag ccatttgccg
     4801 gcaatcgcca cggcaaagct ggccacatgc gccagcaaaa acaccaagag cgacgccgcc
     4861 actgccagag caatgctgtc gtccgggtcg ccggtcaaaa agtggtaagt aaagaacggc
     4921 gccagccact gcaacatgcg caggctgacc aggggcggca gcatggccgc ttgcgccagg
     4981 ccgcacagcg cgcgcttgag cgggctgacc gcggcaaagc tgggcgcgac attggctgcc
     5041 gcctgggcgg acaattgctc cagcgcgctg gccatcgcac ccaccgtgcg ggcgcgataa
     5101 atctcctgca cggtaatgcc ggcatagcgc ggcgtttgcc gcagcgccga caccagccgc
     5161 gccgccagca gcgaatggcc gcctaaatca ttaaaaaaat ccgcctcgcg cgccagtggc
     5221 tggccgggaa acaagcggcg caaatggcca aacagctcgc gctcggcgtc attagtcggt
     5281 tcggcttggt cttccaccgc cggcaaggca tccaactcac gcgcgcgcag cgccttgcga
     5341 tccaccttgc ccgaggtcag ccgtggcatg gcgtccaggg tgatgaacag cgccggcacc
     5401 atatatggcg gcagccgctc gcgcagcccg gcgcgcaagg cggcgacatt ggccggctgg
     5461 ccgctgtcgg tcaccagata ggcaatcagc tggtccactt cgtcgatggc tttgacaatc
     5521 accgccgccg cgccaatatc ggcctgatcg cacagcgccg cttcgatttc gcccagctcc
     5581 acccgaaacc cgcgaatttt cacctggtca tccgcccggc ccaggcactg cacctgaccg
     5641 gtttcgtcga tcttggccag atcaccggtg cggtacagcc ggcggtcgta gccgcgctcg
     5701 ataaacgggt tatccagaaa cttctcggcg gtcagctcgg ggcggcccag atagccgctg
     5761 gccacgccgg ggccgacaat gcacagctcg cccacctcac ccgccggcaa caagcgcata
     5821 tcggccggat ccaccaccaa caggccataa ttgggcagcg gctggccaat ggtcaccggc
     5881 tggccgggca ccagtcgcgc caggctggcc gacacggtgg cctcggtcgg gccgtaggta
     5941 ttgaacaagg gccgctcatg ggtggcccag cgctcaacca atgcctgcgg gcacatctcg
     6001 ccgcccaggt tgatgatgcg caaattgggc acgtcgcggc caaacagcgc cagcaggctg
     6061 ggcaccgcgt gcagcacggt cacgcgctgg cgaatcagcg cctcgggcag cgcgtccggg
     6121 tcgctgacca ggcatttggg cgcaatccac aaggcggcgc caaccagata gctgatccag
     6181 atttcctcga acgacatatc aaacgccagc gagaaaccct gatacaccag gtcgtcctcg
     6241 cgcacgccca acacgctgtt ttcgctgcgc aaaaagtggc agatgctgcc atggctgacg
     6301 gcaatgccct tgggcacgcc agtcgagccg gaggtgtaga tcacataggc cgggtgttcc
     6361 gggcgcaggc cttcgcggcg gcgcaagggg ccgtccactg ccgcttgcag cgcctcgggc
     6421 gtccagcgcg ggagattcac gtcgctcagc cgggcggcca tgtccgggtg gcacaccagg
     6481 cccagcgcgc cagcgtcgcg caggcagatt tccacccgct cgggcggggt gtcggcgtca
     6541 aacggcaacc aggcggcgcc agtcagggtg atggcggcct gcatcaccag cgcctcggcg
     6601 ccgcgcggca tccacaggcc aatatgctgg cccggcgcag cgccggcttg cagcaggtga
     6661 tgcgccgcca ggctggcgcg cgccagcagc tcgccatagc tcaggcagag ctcaccccaa
     6721 tacagcgccg gccggtcggg ctggcgggcg gcggtggctt ccagcaagtc gggcaagccg
     6781 gtgtcagtca aaaaatccgg gcgggaaagt ccgtgcaaaa tcatcgggca ggcgtatcca
     6841 gggctcgcgg caggcgacgg cctgccgggt atgatcggca attgctaata ttggcaataa
     6901 caccttgcgg tgactgcctg tttctggaat aacgcccggc ctgtcatttg gttgacgcgc
     6961 tgtgtaaagc cacgttaagc gccgctcacn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7081 nnnnnnnnnc ctgatccagc gcccgcgccg acagtccgaa caccgcctgg ctgaccgccg
     7141 ccagcaggct gcgccagtcc agggtgaccg gcaaatcgcg cccttcaaac agggcattcg
     7201 cctgcaggcc ggcaaaatcg cccagcatgc gcccttcgcg ccccaggccg ccgccggcca
     7261 atagcgccag gccgccatgg ccgtggtcgg tgccgcgtgt gccgttttcg gcggcggtgc
     7321 ggccaaactc gctgagcacc accacgcggg tgcgcgccca ttctgcctcg cccagcgccg
     7381 ttttcatcgc caacaggccc tggcccagct gtgtcagcgg ctgcgccagg ccggccgcct
     7441 gattgacgtg gctatccacc ccgcttaaat cgacaaaggc cagcgccagt tcgggctgct
     7501 ggcgcagcac ctgacccatc tgccgggctt gcgccgccca gccggccaag ggggcggcgt
     7561 tgcggctggc ctgatccggg tcggcttgcg ccagcagcgc ctgggtgtcg atggcttgtt
     7621 gcagccagcg ctggctgggc gagccggcat gcgcgcgcaa cagcgcctcc tgcaaagcgc
     7681 cgccgggcag gcgcaaggcc tggcgcgcca agggaatatg ctcgatggcc acaccagccc
     7741 catacagggc caagggggca ttggcggcaa aattggcagc cagccggccg tccaactggc
     7801 tggccagccg gcccatccaa ccggtgttgc cgctgatggc gcccgtgccc agctccatca
     7861 cgtcctgcgc ctgaaaatgg ctgcggctgg tgttgggcga gcccgccgtc gggcaaaacc
     7921 acagctcgcc agcccgatac aaggtcaaac agtcggccag cgccgggtgc agggcaaagc
     7981 cgctgccagg caaggccagg ccgctttgca gcaccggctg cgccagtgtc gggcgcaggc
     8041 tgggccagcg ggggtcggcc acgggtgata tggcatgcag gccatccagg ccgccacgca
     8101 gcagcacaaa caccagccgt ccttcacgcg cggccgctgg cgcagcccac gtgcgccaag
     8161 tgaatggcaa taaactggcg gcggtcagcg cgcgcaaagc cgcacgacgc cccgaacaac
     8221 aggacaatac cgacatgaca tgctcacaat cagggggaag ctcacacagg ctgccagctt
     8281 ggcataagcg cgctattcgg gccagtgctg gcgcggggaa tcgttggatg cgctgtcata
     8341 tggtctgacc attagcagcc cacagtcgta taatgtgctc ttttgcacac acaagaggga
     8401 cacaagcatg gtggaaacct ttctggtgtt gattggcttg cagctgctgg gcgaatggct
     8461 ggcgcgctgg ctgcatattc cggtgcccgg cccggtgctg ggcatgttgc tgttgtttgt
     8521 tggcttgtgc ctgcgccgtg gcgtgcccac cgccttgcag cacggcgtgc cggcgttttt
     8581 gcagcatttg tcgctgctgt ttgtgccggc tggcgctggc gtgctgctgt atgcccatct
     8641 gctgaatggc cagaccgtct ggcagctggc cttggcgctg gcggtgggca ccacggtgac
     8701 tttgctggtc agcgccctgc tgctcgccgg gctgatgcgc tggcgcgggg agcacggcca
     8761 tgactgattt tgccagcctg tggcaggcgg cgcgccaaca gccgctcacc tgggtgtttc
     8821 tcaccatgtt gtgctaccga ttggcgattg cgctgaatcg ccaaagcggc ggccacgcgc
     8881 tggccaaccc ggtggggctg ggcgcgctgc tgctgattgg cgtgctgctg gccaccggca
     8941 ccgattacaa acagtatttt gaaggcggca aattcatcca gatgctgctg ggcccggcca
     9001 ccgtggcgct ggcggtgccg ctgtacgcca acctgcaccg gctgtccaag gtgttctggc
     9061 ccttgttgat cagcctgctg ttgggctcgc tggtgggcat tgtctccagc gcgggcgtgg
     9121 cctgggcgct ggggctggat gacattttga tcaaatcgct cttgtcgcgc tcggtatcca
     9181 cccccatctc tatgggtatt gccagcgaaa tcggtggcgc gccggagctg gcggcggtgt
     9241 ttgtgattgt atccggcgtg tttggcgcca tgctgggctg gccgctgctc agccgtttgc
     9301 gtttggggca agatgtggtg tacggttttg ccactggcat ggccgcgcat ggcattggca
     9361 cagcccgggc gtttcagcgt tcagacacgg ctggggcgtt ttctggtctg gcgatgggcc
     9421 tgaatggcct ggtgacggcg attttgattc cgctgctttt gcgcttttgg cattagcgcg
     9481 cagacgcggc agaacagacg tcttcacacg ccggtcacac cgggccggta ggctgccccg
     9541 attgcgccca tagcaattgg ccgcgccgaa atcataataa ggagactcaa ttggatctga
     9601 ttctgtggcg tcatgccgaa gccgaggaag gcagcaacga tctggcccgc gagctgaccc
     9661 gcaaaggcca gcagcaagcc ggcctgatgg ccgcctggct gcgcctgcgc ctgccgcgcg
     9721 attttcagct gctggccagc gaagcccgcc gctcgcaaca aaccgctgcg tttttgtcaa
     9781 aaaactaccg cgtgctgccg gaaatcaacc cggacgccac ggtggaatcc gtacttaaag
     9841 caagccactg gcccgacgcc cgccacccca tcgtactggt gggccatcag ccttatatgg
     9901 gcatgattgc ctcgcaactg attggcggcg aagcacaaat gtggaatgtc aaaaaaggcg
     9961 gcgtgtggtg gatcagccag cgcgtgcgnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10081 nnnnnnnnaa cgccacagcg tggcggggga ggacagcagc aagatggcct gttgcgccgg
    10141 tggcaagcct tccgccgccg cccgcgtggc tgccgtccat ggccccaggc tgcgctcggc
    10201 ggcagcggca tcccagttgc acgcctgggc cgcatctgcc gggccggtca gccgccattg
    10261 ccgctgtgcc agcgcctggg ccagctgtac gcgcaaggcc atgcccagcg ccgtctgcca
    10321 ttcgccggca ttttgtggat agccatctgg cgtcaagcgc aggaatggcg cctgccccat
    10381 cgcctgcaac atattcagca aaggttcggc atgggtgatg ggggcgtcct gacacaccgc
    10441 gcgcgcggta gccagcacgt aatcctgtgg cagacggaac atccgctgct tgagcaggct
    10501 gtcggcaaag gctgggctgg ccagcatcac ccacaaggca ggcgacagtc gcccgccgct
    10561 ttgcacatag gcctgtgcca tgcgctcgac cagcgccgca ggcggcgtat cgccgacaaa
    10621 gcgttgtgcc agcttgcggc tgacatgccg tgccgtggcc gggtgcaagg ccagccggtg
    10681 caagagcgca tccattgccg cgcgaccgtc agccagtgcg tatgtttgcc ccagcacctg
    10741 caccgagcct tcgccacgcc ggcgtggatc cagggcaaag ccaccatcgg caagcgcatc
    10801 cgccgtgccc agatccggcg cactcacccc gctgatcaac cgtgccgtgg cttcgacatc
    10861 cgcctgctga tagcccccgc ccacacccaa ggtatgcagc tccagcagct cgcgcgccag
    10921 attttcgttc agcccggtgc gcccgcgccg gcccagctcg ctgtctgggg cggtggaatt
    10981 gatattgtcc agatagccct gcatggccgt gtggtaaaac gcggcgcgca agagagattc
    11041 aaaacggtca tccgccatgg ccgcctcaat gcgtgaggca taatcatcca ccagccaagg
    11101 caactggcct ttgtgcagat acaccgaaaa atgattcagc cagaaggcca gcaacacctc
    11161 atgtgccgga ttgtcgctgt tggccatcat caatagccgt cgctgaaccg tagccgactt
    11221 catggcattg ccctggcggg ccatggattg caccagcgga atatcctgcg ccttgcgtgc
    11281 ttcttgtacc cggaccgggc catattgcgc ccagcgctcg gccagggggg gcgcatcggc
    11341 cagcgcatcc agcgcggtgc ggacagcagg agccacaccg ctgggtgcgc gccagccgcg
    11401 ctgcaccatg gcccgaggcg tttgcccggt aagcgccgcc acactggcat cggtggcgcc
    11461 atagccccag cgcgccagca tggcccgcgc cagtggcgca tccatcgggg tgtccagctt
    11521 cagtggcccc gccgcctgcg tcagcgtggc cagcatgcac gccaccccgc ccagcacccg
    11581 cattgcttgt ttcatcgttg gctccgcatt tcatcacagc acagcattat cctgcccgac
    11641 ggtgatttcg gccatggggg cgcttgcggc cccttgcctg acaattcggg ccgactgcgg
    11701 cacaatgccg ctttagcctg ccctccatcc tgactctgcc atgtctgctg atttttttct
    11761 gcgcccgcac tgggcggccc ccgcccatgt gcatgccctg tccaccaccc gccagggcgg
    11821 ggtcagccag ccgccttacg ccagctttaa cctgggcgct catgtcggtg acgacccggc
    11881 cgccgtggcc gccaaccgcg cgctgctgcg ccagcaactg cccgccgagc cggcctggct
    11941 gaaccaggtg cacggcatcc atgtggtgga cgccgccagc gtggccggca cgccggtgga
    12001 cgccgacgcc agcttcagcc gcacgccggg cgtagtgtgc gcggtgatga ccgctgactg
    12061 cctgccggtg ttgttcaccg accgtgccgg cagcgtggtg gccgccgccc atgccggctg
    12121 gcgcggcctg ctggccggtg tgctggaagc caccctggcc gccatggcct gcccggcgca
    12181 cgaggtgctg gcctggtgtg gcccggccat cagccgcgat gcctttgaag tgggcagcga
    12241 agtgcgcgcc gcctttgtgg cgcacaaccc ggccgcagcg gcggcgtttt cgccgctgcc
    12301 agacggcaaa tggctggccg acctgcccgg cctggcccgc cagcgcctgc tggcggccgg
    12361 cgtgccggac aatcagtgtg tggacagcga gctgtgcacg gtgatcgagc gcgagcagtt
    12421 tttttcatac cgccgcgacg gctgcaccgg ccgcatggcc agcctgatct ggctggcgcc
    12481 tgctgtgtga aacgtttata ttgatcggtt gccgattttg cccgcaggag tgcttccatg
    12541 atcaaagcca ttgtcagcgg ccacagccgt ggcctgggcg ccgccattgt cgaggccttg
    12601 ctggcccgtc agttgccggt gctgggcctg gcgcgcagcg ccctgcccgc acgcgccggg
    12661 ctcagccaat gcgccctgga cctggccgac ccggccgcgc tcaccgcctg gctggctggc
    12721 cccacactgc atgactggct ggccgacgcc agcgccgtct ggctggtgaa caacgccggc
    12781 caggtgcagc ccatcgccgc actgggcgaa caagacccac aggccatcgt gcaggccatc
    12841 aatcttaacg tcaccgcccc gctattgctc agcaatgccg tgctggccgc cagcccgcac
    12901 tgtgttgagc gccgcctgct gcatgtgtcc agcggcgccg cccggcacgc ctaccccggc
    12961 tggagtgtgt attgcgccag caaggccgcg ctggaccacc acgcccaggc cgtggccgcc
    13021 gaacgccaca ccggcgtgcg cctggtcagc ctggcccctg gggtgatcga caccgagatg
    13081 caggccgaca tccgcgccgc cagcctggcg cagtttccgc tgcgcgaacg ctttgacgcc
    13141 ctgaaagcca acggccagct caccagcccg caaacctgcg ccgagcacct ggtggccgcc
    13201 ctggcccacc cggcatttgg cgaagtggtg ctcaccgacc tgcgccacgg ctgaacacat
    13261 ccagcctgcg gcgggccgtt tggcaaacca catcccgccg catccacgct acaatccccc
    13321 cggaatgatt gtctgattca agccgaggtt tgccaccatg actgcaccca cttcttccgc
    13381 cagctttgcc gtgattggca gttcgtccag cgccaccacc aatgcgctga tttccggctt
    13441 caaatggggc agcagcacgg tgggcagcag cgcctcgctg agctacagct ttccctgggc
    13501 cagcggcggc agcgccacct ttgccgggct gaacgggcaa agctactccg acctgaacga
    13561 acaaaacacc ggcacggcgc tgaacaccac ccagcaagcg gcgtttcgca gcgcgctgca
    13621 agcctgggcc aatgtcgcca acctcagctt tagcgaagtc agcgacaccc gcagcaatgt
    13681 gggcgatatc cgcgtagcct ggacctccgc cgcctatggc atcagcaccg gcgagcaagc
    13741 ctggggctgg gcaggctatc cgggcgcctg gcccagcgcg ggcgatgtgt ggatttcggc
    13801 gcggggcagc gacaccgcca gccaatcctg ggcggccggc tcgtataact tcgaagcgct
    13861 gatgcacgaa ctgggccacg ccctgggcct gaagcacccg tttgaagact cgcccaccct
    13921 ggcaaaaagc ctggacaccc gccaatacac gctgatgtcc tacacc
//
LOCUS       DAOPYX010000039        13567 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_4983_length_13567_cov_7.660820, whole genome shotgun sequence.
ACCESSION   DAOPYX010000039 DAOPYX010000000
VERSION     DAOPYX010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 13567)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13567)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13567
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4983_length_13567_cov_7.660820"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(81..1034)
                     /gene="lipA"
                     /locus_tag="PKN55_03410"
     CDS             complement(81..1034)
                     /gene="lipA"
                     /locus_tag="PKN55_03410"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008953896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HNX70326.1"
                     /translation="MKTDNTQGVKLKGEAKTARIPIKVVQLDERLKKPEWIRVKSPSG
                     ARFHEIKDILRQQKLHTVCEEASCPNIGECFGSGTATFMIMGDICTRRCPFCDVGHGR
                     PNPLDPNEPRHLAETVAALRLKYVVITSVDRDDLRDGGAGHFADCIREVRALSPDTKI
                     EVLVPDFRGRLDIALDILSETRPDVMNHNLETAPRLYKQARPGADYQHSLELLKAYKA
                     RNPDVATKSGIMVGLGETDEEVYEVMRDMRAHDIDMITIGQYLQPTTTGHLPVLRYVH
                     PDVFKQFETEAYAMGFRHAAVGALVRSSYHADQQAHQAAAV"
     gene            complement(1060..1665)
                     /gene="lipB"
                     /locus_tag="PKN55_03415"
     CDS             complement(1060..1665)
                     /gene="lipB"
                     /locus_tag="PKN55_03415"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009116829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HNX70327.1"
                     /translation="MLIKHLGMVDYEPTWRAMQAFTDARDEHTPDELWLVQHPPVYTL
                     GLAGKPEHLLCATDIPLVKTDRGGQITYHGPGQLVVYMMIDFKRLGLGVRELVRHIEQ
                     AVIDLLAEFGVTGNGDVDAPGVYVDGAKIASLGLRIRNHATYHGLSLNVDMDLTPFSW
                     INPCGYSGLRVTQLRELGVTLDVNAVAERLLPHLRAQLAAH"
     gene            complement(1687..1962)
                     /locus_tag="PKN55_03420"
     CDS             complement(1687..1962)
                     /locus_tag="PKN55_03420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF493 domain-containing protein"
                     /protein_id="HNX70328.1"
                     /translation="MTQTPDTPPLLEFPCRFPIKIMGAAHPEFTRTVLEVTRIHAPDF
                     EEVDLVVRESSGGNYLAATITVRATSREQLDDLYRAFSGHPMVKMVL"
     gene            3041..4150
                     /locus_tag="PKN55_03425"
     CDS             3041..4150
                     /locus_tag="PKN55_03425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014088055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNX70329.1"
                     /translation="MSVASPDTDVLVSFRGVQKSYDGENLIVKHLDLDIRRGEFLTLL
                     GPSGSGKTTCLMMLAGFETPTAGEIRLDGKLLNRVPPHKRNIGMVFQNYALFPHMTVG
                     ENLAYPLKIRKLPAAEVDAKVKRALGMVKLDKFANRYPGQMSGGQQQRVALARALVFE
                     PQLVLMDEPLGALDKQLREHMQLEIKHIHENLGVTVVYVTHDQSEALTMSDRVAVFDK
                     GEIQQIDPAETLYEAPVNSFVAGFIGESNAISGTIEHVDGDFCQARLIGGGSVSARAV
                     CVRQAGQPTTLSIRPERVMLGERAVGCANRFDATIAEFIYLGDHLRLRLDFAGNSGFM
                     VKVPVPEVDPAWQVGNRLSIGWRAEDLRALDPLVV"
     gene            4250..5287
                     /locus_tag="PKN55_03430"
     CDS             4250..5287
                     /locus_tag="PKN55_03430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018152307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNX70330.1"
                     /translation="MKKMIQLAAVAAVASVFAGQAVARDLTVVSFGGANKEAQVAAFY
                     QPFEKATGQKVVGVDYNGEVAKVKAMVEAGKVTWDVVEVESPEVLRGCEEGVYEKIDP
                     KLIGGKAAFLPGTVSECGVGIFVWSTAIAYNADKLKTGPTSWADFWDVKKFPGKRGLR
                     KGAKYTLEFALLADGVKPADVYKVLGTKAGVDRAFKKLDQLKANIQWWEAGAQPPQFL
                     ASGDVVMSSAYNGRIDAAQKEGKNLKIVWNGSIWDIDSWVIPKGSPNKEQALKFVQFA
                     SKPENQKTYSHKIAYGPTNKSAVGMIDPKVLPSLPTAPANLNGALGINVQFWVDHGEE
                     LEERFNAWAAK"
     gene            5477..6733
                     /locus_tag="PKN55_03435"
     CDS             5477..6733
                     /locus_tag="PKN55_03435"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNX70331.1"
                     /translation="MNMTADAPLLAADGVPLAVKLKRAERRKKLRAALLVAPLAVFLL
                     ATFILPIALLLYRSVENPEVPAMLPKTVSALAGWQGQGLPDEAALGVFVQELTAAKDN
                     KAMVGNAAKRLNMEISGYRSLLTKTVRAMPLDPAAGPLREQFVNIDERWAEADYWRVL
                     RHQDSRYTGFYLQSSLDIKRGADDSLHMAGDDEAIYLKIFGRTLWMSAVVTLFCFLLG
                     YPLAYWLVNLPTRKSNLLMIVVLLPFWTSVLVRVAAWIVLLQNEGLINGALMAMGFVD
                     SPVQLAFNRIGVYIAMVHILLPFMILPVFSVMKAIPPTYMRAAVSLGSHPIPAFWKVY
                     FPQTVAGVGAGALLVFIMSIGYYITPALLGGPDDQMVSYYVAFFTNNTVNWGMAAALG
                     SLLLIATMALYTLYARIVGANRLSLG"
     gene            6749..7579
                     /locus_tag="PKN55_03440"
     CDS             6749..7579
                     /locus_tag="PKN55_03440"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNX70332.1"
                     /translation="MLQPYATPLEKLWFYAFRLFCIGVLVFLILPLLVIVPLSFSADS
                     FLMYPIHSFSLRWYEAFFTNSEWMDSLKNSMIVAPAATLLATVLGTVAAVGLTSADFP
                     GKSFMMTVLISPMVVPVVIVGVGAYLFFAPLGLANSYLGLVMVHAALGVPFVVITVSA
                     TLRGFNPNLVRASASLGGNPVYTFFRVTLPLIAPGVISGGLFAFAASFDEVVVTLFLA
                     GPEQTTLPRQMFAGIKENISPTIAAAATVLILFSIVLLLTLEWLRGRSEKMRTAASAA
                     "
     gene            7863..8855
                     /locus_tag="PKN55_03445"
     CDS             7863..8855
                     /locus_tag="PKN55_03445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014086427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HNX70333.1"
                     /translation="MKHALLALATLSALSAVAHAEEVVVYSARNEQLIKPLFDAYKKE
                     TGVDVKFVTGQEGPLMARLKAEGANTPADILLTVDAGNLWQAAHEGLLKPVQSKVLDA
                     NVPAHLRDPKGQWFGLSVRARTIFFNTHKVKPAQLSSYEDLAEAKWKGKLCLRTSKKV
                     YNQSLVGMMMADIGPVKTERVVRGWVDNLATDVFPDDTKMLEAIAAGQCDVGIANTYY
                     FGRLMEKNPSLPIGIFWANQQGSGTHVNVSGAGVTRYAKNEKGAVRLIEWLSSEKAQN
                     LYADVNMEYPVNRKVNVDPVVAKWGDFKHNYINVAKAGELQAEAVKLMDRAGYR"
     gene            9026..10708
                     /locus_tag="PKN55_03450"
     CDS             9026..10708
                     /locus_tag="PKN55_03450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011135461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HNX70334.1"
                     /translation="MHGETSSPATTYSPGWRKPAWWPAVSRWQALAALISSVTVLPLL
                     VIALSFADVNADIWAHLSEFVLPRLLANTALLLLGVGVGVLLLGVPLGWLVAVHEFPG
                     RRVFNWALMLPLAMPAYVMAFTQIGLLDFSGVLQSWLREVWGSSAWFPPIRSRGGVVL
                     VMSLALYPYVYLLARDAFASQGRRALEAAQSLGLSPWQGFWRVALPMARPWLIGGVSL
                     ALMEALADFGTVSIFSFDAFTTAIYKAWFAMFSLSSASQLSSLLVLLVFALIWLEQRL
                     RGGRAYHAGRLAQPQPRLPLHGWPAALACGFASLVLLLAFVLPFGQLLVWVARVWEAD
                     LDARYIDFVWHSLALAIMAAGLVTLCALALSYAQRRDPSPTARLWVRLATLGYAVPGT
                     VLAVGLFIPVAALDNLLIAWLGAALPEGTTAIFKGTLAVMLLAYLARFAAVGFSSVDA
                     AMQRITRSQEETARSLGVAGFALLRRVHVPMLRGGLLTAVLMVCVDVMKEMPITLMTR
                     PFGWDTLAVRIFEMTSEGEWERAALPAVALVLVGLLPVLLLARAPSHTGARR"
     gene            10705..11754
                     /locus_tag="PKN55_03455"
     CDS             10705..11754
                     /locus_tag="PKN55_03455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012698081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNX70335.1"
                     /translation="MTSSPLLQLDAISQRYARHSVVEQLSLSLQRGEIGCLLGSSGCG
                     KTTVLRCIAGFEPLSGGRILIDGQLLADARLHTPAHKRQIGMVFQDYGLFPHLTVAGN
                     VGFGLDALARDARAAQVARMLALVGLDGLAQRYPHELSGGQQQRVALARALAPQPRLL
                     LLDEPFSNLDVSLRERLGQEVRDILKAAQATAILVTHDQHEAFAMADKVGVMEQGRIM
                     QWDSPYNLYHQPANRMVADFIGQGVLLPGEVCADACIRIELGEACSLRPHGFAAGSRV
                     EVLLRPDDVAHDDASPLRARVAARAFRGAEFLYTLALPSGQTVLSLAPSHHDHAIGEE
                     IGVRLDAEHLVAFAV"
     gene            complement(11765..12370)
                     /locus_tag="PKN55_03460"
     CDS             complement(11765..12370)
                     /locus_tag="PKN55_03460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018749323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE/ArgO family amino acid transporter"
                     /protein_id="HNX70336.1"
                     /translation="MQSAVFFQGLAVSAGLIVAIGAQNAFVLAQGVRGAHRLQIALAC
                     ASSDALLIALGVGGAGALIASQPALLALARWGGAAFLLVYAAMALRRAWHGESMTLPA
                     DSQQSVWRVLLATLAVTWLNPHVYLDTVVLLGGVAAQLPAQERFTFGLGAALASWLWF
                     FALAYGARLLAPLFTRPTSWRVLDISVALVMLTVAYQLLQG"
     gene            12477..13373
                     /locus_tag="PKN55_03465"
     CDS             12477..13373
                     /locus_tag="PKN55_03465"
                     /inference="COORDINATES: protein motif:HMM:NF002964.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator ArgP"
                     /protein_id="HNX70337.1"
                     /translation="MLDAKPLDALEAIVRAGSFELAAQWLCITPSAVSQRIRQLEEHL
                     GQTVLVRSQPVRPTPAGKRLLRHVRQLQLLEAELRHDLAGDVPDAFTTLAVAVNADSL
                     ETWFPEAIADCVAQENLLLQLIVDDQDYTHALLKNGDVIGCVTTQAQALTGCQSVCLG
                     RMPYVCVATPAFARRYFADGVSAEALRAAPAILFNLKDSLHLNFMADYGLALNDFPYF
                     TVPAVHALLNVVLAGLGYGLTPRLQVADLLASGRLVDVLAGAPGPALTLYWHSWALQS
                     PKLARFGAAVQAGAAQAMPPAL"
BASE COUNT         2378 a         4105 c         4349 g         2735 t
ORIGIN      
        1 gggtgtcgtg agcgaagaca agaaaagggg cgagccttgc actcgcccct gatcacaccc
       61 aaaggatttt taggtgctct ttatactgcc gccgcctgat gcgcctgctg atcggcgtgg
      121 taagacgagc gcaccagcgc gcccaccgcc gcgtggcgga agcccatggc gtaggcttcg
      181 gtttcaaact gcttgaacac gtccgggtgc acgtagcgca gcaccggcag gtggccggtg
      241 gtggtcggtt gcaggtattg gccgatggtg atcatgtcga tgtcgtgggc gcgcatatcg
      301 cgcatcactt cgtagacttc ttcgtcggtt tcgcccaggc cgaccatgat gccggatttg
      361 gtggccacgt ccgggttgcg cgccttgtag gctttcagca gctccagcga gtgctggtaa
      421 tcggcgccgg ggcgggcttg cttgtacagg cgcggcgcgg tttccaggtt gtggttcatc
      481 acgtccgggc gggtttcgct gaggatgtcc agggcgatgt ccagccgacc ccgaaaatcc
      541 ggcaccagca cttcgatctt ggtgtctggc gacagggcgc gcacttcgcg gatgcagtcg
      601 gcaaagtggc cggcgccgcc gtcgcgcagg tcgtcgcggt ccacgctggt gatcaccacg
      661 tatttcaggc gcagcgcggc cacggtttcc gccaggtggc gcggctcgtt cgggtccagc
      721 ggattggggc ggccgtggcc gacgtcgcag aacgggcagc ggcgggtgca gatgtcgccc
      781 atgatcatga aggtggcggt gccgctgcca aagcattcgc cgatgttcgg gcagctggct
      841 tcttcgcaga cggtgtgcag cttttgctgg cgcaggatgt ccttgatttc atgaaagcgc
      901 gcgccagacg gggatttgac gcggatccat tcgggttttt tcagccgttc gtccagctgc
      961 accactttga tggggatgcg cgcggttttg gcttcgccct tgagcttcac gccttgtgtg
     1021 ttgtcggttt tcatgttgtc tccagtgggg gcgggcgcgt taatgcgccg ccagttgcgc
     1081 gcgcagatgc ggcagcaggc gctcggccac ggcgttgaca tccagtgtca cgcccagctc
     1141 gcgcaattgg gtgacgcgca ggccgctgta gccgcacggg ttgatccagc tgaacggggt
     1201 gaggtccatg tccacattca ggctcaggcc gtgataggtg gcgtggttgc ggatgcgcag
     1261 gcccagcgag gcgattttgg cgccgtccac atacacgccg ggcgcatcga catcgccatt
     1321 gccggtaacg ccaaattcgg ccagcagatc aatcaccgct tgttcaatat ggcgcaccag
     1381 ctcgcgcacg cccagcccca ggcgcttgaa gtcgatcatc atatacacca ccagctggcc
     1441 ggggccgtgg taggtgatct ggccgccacg gtcggttttc accagcggaa tatcggtggc
     1501 gcacagcaga tgctcgggct tgccggccag gcccagggtg tacaccggcg ggtgctgcac
     1561 cagccatagt tcgtccgggg tgtgttcgtc gcgggcgtcg gtaaacgcct gcatggcgcg
     1621 ccaggtgggc tcgtaatcca ccatgcccag gtgtttgatc agcataggac ggctcgcagg
     1681 gtgaggttac aacaccatct tgaccatggg gtggccgctg aaagcgcgat acaaatcgtc
     1741 cagctgctcg cgcgaggtgg cgcgcacggt gatggtggcc gccagataat tgccgccgct
     1801 gctttcgcgc accaccagat cgacttcttc aaaatccggg gcgtgaatgc gggtgacttc
     1861 cagcacggtg cgggtgaatt caggatgcgc cgcgcccatg atcttgatcg ggaaacgaca
     1921 gggaaattcc agcaacggag gggtatcggg ggtttgggtc atggtgagcc tcggcgccaa
     1981 atccgccata cggtggcggt gcggttgttc aaaccgggaa gatgcgccca ataggaaaaa
     2041 atttcaatat tgaacgcgaa atgttgtgtt gggaaagcgc cggcccatct aggccagcac
     2101 cctgccaggc aagcaggaca gttccaggca acaccccggc cacaaacagc cactgaagcc
     2161 ctggtctgcg acaccacaca gcggcatcgg cacattcagc cagcaacatc atataggtgg
     2221 ttagcgttgc gcctcccttg gcgttgttgc gcaaccttgc cgtaccactt gtactgtctg
     2281 cggccgcccg cccagtcagg atcgctgcgc tgatttttgt gagcggcgca tatctataag
     2341 agcatctggt tcacctgccc gatacgcccg cccgttgccg acaggtcaga ccagcggaaa
     2401 gacgatggca aaacccggaa tggatacacg ataacagttt gtcgtggcct gtgtcgcaag
     2461 tgacatgtca gatgaacacg accgtgtgct taatctttta aacacctcac gtgggcggcc
     2521 atgcagccgg caaggtgaat catgcgttcg agttaacgtc aatgttgcta tgcaacattt
     2581 tattgaaaat cgacaatgtg ttgtgtgcat gcaacatctg ggtgttcgat taattcaacg
     2641 ctttatgaac actggttcat taaatatgca ccaaacaagt gcaaaaccgc acaaaaagcg
     2701 cgcattccca tgggttttct cggtattttt gccgactcca gtggggtgtt tgccccggat
     2761 gagtgtttaa tggatcagcg ttttgataaa tttattaaac actcgcggtt ttctggcatg
     2821 cttctcgctc taagtctggc cgttaaggca gcacccttgt catggtgtca gtgacctggg
     2881 tgaccgggca ggatgcaggc ggattttctg cggcgagtgc cgctggcatg acgcaaaatg
     2941 ctttacaagc aaaagcgcat ccccgtaatc ttgcctcaca aaaatagaat tcgattccgc
     3001 attgcggaac agtggcagcc cctaagagga gatcatttca gtgtccgttg cctcccctga
     3061 taccgatgtg ctggtgagct ttcgtggcgt gcaaaaaagc tacgatggcg aaaacctcat
     3121 cgtgaaacac ctcgacctcg acatccgtcg tggcgagttc ctcactctgc tggggccgtc
     3181 cggctcgggc aagaccacct gcctgatgat gctggcgggt tttgaaaccc ccaccgccgg
     3241 cgaaatccgt ctggatggca agctgcttaa ccgcgtgccg ccgcacaagc gcaatatcgg
     3301 catggtgttc cagaactatg ccttgttccc gcacatgacg gtaggcgaaa acctggccta
     3361 cccgctgaaa atccgcaagc tgcccgccgc cgaagtggac gccaaggtca agcgcgcgct
     3421 gggcatggtc aagctggata aattcgccaa ccgctacccc ggccagatgt ccggcggcca
     3481 gcagcagcgc gtggcgctgg cgcgggcgct ggtgttcgag ccgcagctgg tgctgatgga
     3541 cgaaccgctg ggcgcgctgg ataaacagct gcgcgagcat atgcagctgg aaatcaagca
     3601 cattcacgaa aacctgggcg tcaccgtggt gtacgtcacc cacgaccaat ccgaagcgct
     3661 gaccatgtct gaccgcgtgg cggtgttcga caagggcgaa atccagcaga tcgacccggc
     3721 ggaaacgctg tacgaagcgc cggtcaacag ctttgtcgcg ggcttcatcg gcgagagcaa
     3781 cgccatttcc ggcaccatcg aacatgtgga cggcgatttc tgccaggcgc gcctgattgg
     3841 cggcggcagt gtctcggccc gcgcggtgtg cgtgcgccag gccggccagc cgaccacgct
     3901 gtccatccgc cccgagcggg tgatgctggg tgagcgcgcg gtaggctgcg ccaaccgctt
     3961 tgacgccacc atcgctgaat tcatttatct gggcgaccac ctgcgcctgc gcctggactt
     4021 tgccggcaac agcggcttta tggtcaaggt gccggtgccg gaggttgacc cggcctggca
     4081 agtgggcaat cgcctgagca ttggctggcg ggctgaggat ttgcgcgcgc tcgacccgtt
     4141 agttgtttga aacctgaacc cggcaacggg cgatggttaa agcatgcagc actgttgtat
     4201 cccctaagca acgtagcacc cctactgtcc ctcaagagga gagaaccgca tgaaaaagat
     4261 gatccagctg gctgctgtgg cagccgtcgc cagtgtgttc gccggccagg ccgttgcgcg
     4321 cgatctgacc gtggtgtcgt ttggcggcgc caacaaggaa gcccaagtgg cggccttcta
     4381 ccagccgttt gaaaaagcca ccggccagaa agtggtgggc gtggactaca acggcgaagt
     4441 ggccaaggtc aaagccatgg tcgaagccgg caaggtgacc tgggatgtgg tggaagtgga
     4501 atccccggaa gtgctgcgcg gatgtgaaga aggcgtttac gaaaaaatcg acccgaaact
     4561 gatcggcggc aaggcagctt tcctgccggg caccgtgtcg gaatgcggcg tgggtatttt
     4621 tgtgtggtcc accgccatcg cctacaacgc cgacaagctg aaaaccggcc cgaccagctg
     4681 ggcggatttc tgggacgtga agaaattccc gggcaagcgc ggcctgcgca agggcgccaa
     4741 gtacaccctg gaattcgcac tgttggctga cggcgtgaag ccggccgatg tgtacaaggt
     4801 gctgggcacc aaggccggtg tggatcgcgc cttcaagaag ctggatcaac tcaaggccaa
     4861 catccaatgg tgggaagccg gcgctcagcc gccgcaattc ctggcctctg gcgatgtggt
     4921 gatgagctcg gcctataacg gccgcatcga cgccgcccag aaagaaggca aaaacctgaa
     4981 gatcgtctgg aacggctcga tttgggatat cgactcctgg gtgattccga agggttcgcc
     5041 gaacaaagag caggcgctga agttcgtgca gttcgccagc aagccggaaa accaaaagac
     5101 ctactcgcac aaaatcgctt acggcccgac caacaagtcc gccgttggca tgatcgaccc
     5161 gaaggtgctg ccgtcgctgc cgaccgcccc ggccaacctg aatggcgcac tgggcatcaa
     5221 tgtgcagttc tgggttgacc acggcgaaga gctggaagag cgcttcaacg cctgggccgc
     5281 caagtaagac tcacgccggg cgggcgcccg gcgcgacttc ttgtggttca agggttgccg
     5341 gcttggccgg cggccgttgt ctggcctgag cggtgatgct gcttgctcgg gtcttgggag
     5401 atggtggggc catctcccgc tggggccccg tcggggggcg gggccctggc gcctcgtatg
     5461 attggaacgc ctgttcatga acatgactgc tgatgctccg ctgctggccg ccgatggcgt
     5521 gccgctggcg gtcaaactca agcgcgccga acggcgtaag aagcttcgcg ccgccctctt
     5581 ggtggcgccg ctggcggtat ttttgctggc gacctttatt ctgccgattg ccttgctgct
     5641 gtatcgcagc gtggaaaacc cggaagtgcc ggccatgctg cccaaaaccg tcagcgcgct
     5701 ggccggttgg caaggccagg gcctgcccga cgaggctgcg ctgggcgttt tcgtccagga
     5761 gctgactgca gccaaagaca ataaagccat ggtcggcaac gccgccaagc gcctgaatat
     5821 ggaaatcagc ggctatcgca gcctgctgac caaaaccgtg cgcgccatgc cgctggaccc
     5881 ggccgccgga cccttgcgcg aacaattcgt caatattgac gagcgctggg ccgaagccga
     5941 ctactggcgc gtgctgcgtc accaggacag ccgttacacc ggtttttatc tgcaatcctc
     6001 gctggacatc aagcgcggcg ctgacgacag cctgcacatg gccggcgacg acgaagcgat
     6061 ttatctgaag atttttggcc gcacgctgtg gatgagcgcc gtggtcaccc tgttctgctt
     6121 tttgctgggc tatccgctgg cctactggct ggtcaacctg cccacccgca aatccaatct
     6181 gctgatgatt gtggtgctgc tgccgttctg gacgtcagtg ctggtgcggg tggcggcgtg
     6241 gattgtgctg ctgcaaaacg aaggcctgat caatggcgcg ctgatggcga tgggctttgt
     6301 cgattcgccg gtgcaactgg cctttaaccg catcggcgtg tacatcgcca tggtgcacat
     6361 cctgctgccg ttcatgattc tgccggtgtt cagcgtgatg aaagccattc cgcccactta
     6421 tatgcgcgcc gccgtgtcgc tgggcagcca cccgattccg gccttctgga aggtgtattt
     6481 tccgcaaacc gtcgccggcg tgggcgcggg cgcgctgctg gtgttcatca tgtccatcgg
     6541 ctactacatc accccggcgc tgctgggcgg cccggatgac cagatggtca gctactacgt
     6601 ggccttcttc accaataaca cggtgaactg gggcatggcc gccgcactgg gcagcctgct
     6661 gctgatcgcc accatggccc tgtacacgct gtatgcgcgc attgtcggcg ccaaccgcct
     6721 gagcctgggt taagaaggag atttgaccat gctgcaacct tacgcaaccc cgctggaaaa
     6781 actgtggttt tacgccttcc ggctgttctg catcggcgtg ctggtgtttc tgatcctgcc
     6841 cttgctggtg attgtgccgc tgtcgttttc ggccgactct ttcctgatgt acccgattca
     6901 cagcttctcg ctgcgttggt atgaagcgtt tttcaccaat agcgaatgga tggactcgct
     6961 gaaaaacagc atgatcgtcg ccccggcggc caccctgctg gccaccgtgc tgggcaccgt
     7021 ggcggccgtg ggcctgacca gcgcggattt tccgggcaag tcgttcatga tgaccgtgct
     7081 gatctcgccg atggtggtgc cggtggtgat tgtcggcgtg ggcgcctatc tgttctttgc
     7141 cccgctgggc ctggccaaca gctatctggg cctggtgatg gtgcatgccg ccctgggcgt
     7201 gccgtttgtg gtgatcaccg tctccgccac cctgcgcggc tttaacccca atctggtgcg
     7261 cgccagcgcc agcctgggcg gcaacccggt gtacaccttc ttccgcgtca ccctgccgct
     7321 gatcgcgccg ggcgtgatct ccggcggcct gtttgccttt gccgcctcgt ttgacgaagt
     7381 ggtggtcacc ctgttcctgg ccggcccgga acaaaccacc ctgccgcgtc agatgtttgc
     7441 cggcatcaag gaaaacatca gccccaccat cgccgccgcc gcgactgtgc tgattttgtt
     7501 ctccatcgtg ctgctgctga cgctggaatg gctgcgcggc cgcagcgaaa aaatgcgcac
     7561 cgccgcctcg gcagcgtgag cgtaatcagc aatcaattga tgtaatcgtt tctctccacg
     7621 tcgttcgttt tccctccttg cacaaccgac gtcgccccag ccagtgggcg gcacaggttg
     7681 tgcttttttt tgtgccgtgt agatgcccgg aatgggccat tgccatgtgc cagcgccgcc
     7741 acaaagtggc atgaattgtc cagtgcttga cggcggtcaa tgcatcgcta attgataatc
     7801 cctatctttt gcgttaggat aaagccatcc gcccattgac ccttagctga ataggtgtgc
     7861 ctgtgaaaca cgctttgctt gcccttgcca cgttgtccgc cctgtctgct gtcgcccatg
     7921 ccgaagaagt ggtggtgtac agcgcgcgta acgaacagct gatcaaaccg ctgtttgacg
     7981 cctacaaaaa agaaaccggc gtggacgtca aattcgtcac cggccaggaa ggcccgctga
     8041 tggcgcgcct gaaggccgaa ggcgccaaca ccccggcgga tattctgctg accgtggatg
     8101 ccggcaatct gtggcaggct gcgcatgaag gcctgctcaa gccggtgcag tccaaggtgc
     8161 tggacgccaa tgtgccggcc cacctgcgcg accccaaggg gcagtggttt ggcttgtcgg
     8221 tgcgcgcgcg cacgattttc ttcaacaccc acaaggtgaa gccggcgcaa ttgtccagct
     8281 acgaagacct ggccgaggcc aagtggaaag gcaagctgtg tctgcgcacc tcgaaaaagg
     8341 tgtacaacca gtcgctggtg ggcatgatga tggccgatat cggcccggtg aaaaccgagc
     8401 gcgtggtgcg cggctgggtg gataatctgg ccaccgatgt gtttccggat gacaccaaga
     8461 tgctggaagc catcgccgcc ggccagtgtg atgtcggcat cgccaacacc tattactttg
     8521 gccgcctgat ggagaaaaac ccctcgctgc cgatcgggat tttctgggcc aatcagcaag
     8581 gctcgggcac gcatgtgaat gtgtcgggcg cgggcgtcac ccgctacgcc aaaaacgaaa
     8641 aaggcgcggt gcgcctgatc gaatggctgt cctcggaaaa agcccagaac ctgtacgccg
     8701 atgtgaacat ggaatacccg gtcaaccgca aggtgaacgt ggacccggtg gtggccaaat
     8761 ggggggattt caagcacaac tacatcaatg tggccaaggc gggcgagctg caagctgagg
     8821 cggtcaagct gatggaccgc gcgggttatc gttgattcca ggcagcaaaa cggcgtcacg
     8881 cgggttgatt gtcagcgctg gcgcagtgtt tgctgcgcac tgaatgccgc accctgaaac
     8941 tgggcgtgcg gcattgtgca ttttggcccg atggcaagca ggtttgtctt ggccttgact
     9001 taaattcttt atcaggaacc ccgccatgca tggcgaaacc tcctctcctg caacgaccta
     9061 ctcgcccggc tggcgcaagc cggcgtggtg gcccgctgtg tcgcgctggc aggcgctggc
     9121 ggcgctgatc agcagcgtca ccgtgttgcc gctgctggtg attgccttgt cgtttgccga
     9181 cgtcaacgcc gacatctggg cgcatttgtc cgagtttgtc ttgccgcgcc tgctggccaa
     9241 caccgccttg ttgctgctgg gcgtcggcgt cggggtgctg ctgctgggcg tgcccttggg
     9301 ctggctggtg gcggtgcatg agtttcctgg ccggcgggtg ttcaattggg cgctgatgct
     9361 gcccttggcc atgccggcct atgtgatggc cttcacccag attggcctgt tggatttttc
     9421 tggcgtgctg caaagctggc tgcgcgaggt gtggggcagt tcggcgtggt ttccgcccat
     9481 ccgctcgcgc ggcggcgtgg tgttggtgat gagcctggcg ctctacccct atgtgtatct
     9541 gctggcgcgc gacgccttcg ccagccaggg ccggcgcgcg ctggaggcgg cgcagtcgct
     9601 ggggctcagc ccctggcagg ggttttggcg ggtggcgctg ccgatggcgc ggccgtggct
     9661 gattggcggc gtgtcgctgg ccttgatgga ggcgctggcc gattttggca cggtgtcgat
     9721 tttcagtttc gacgccttca ccacggcgat ttataaagcc tggttcgcca tgttctcgct
     9781 gtcgtccgcc tcgcagctgt cgtctttgct ggtgctgctg gtgtttgccc tgatctggct
     9841 ggagcagcgt ctgcgcggcg gccgcgccta ccacgccggc cggctggcgc agccacagcc
     9901 gcgcttgccg ctgcatggct ggcctgcggc gctggcctgc ggttttgcca gcctggtgct
     9961 gctgctggct tttgtgctgc cgtttggcca attgctggtg tgggtggcgc gggtgtggga
    10021 agccgacctg gacgcccgtt atatcgactt tgtctggcat tcgctggcgc tggccatcat
    10081 ggccgctggc ttggtgacgc tgtgcgcgct ggccttgtcc tacgcccagc gccgcgaccc
    10141 ctcgcccacg gcgcggctgt gggtgcgcct ggccaccctg ggctatgccg tgcccggcac
    10201 ggtgctggcg gtgggtttgt tcatcccggt ggcggcgctg gataatctac tgattgcctg
    10261 gctgggcgcc gccctgccag aaggcaccac ggcgattttc aagggcacgc tggcggtgat
    10321 gctgctggcc tatctggcgc gctttgccgc cgtggggttt tcttcggtgg acgccgccat
    10381 gcagcgcatt acccgcagcc aggaagaaac cgcccgcagc ctgggcgtgg ctggctttgc
    10441 gctgttgcgc cgcgtgcatg tgccgatgct gcgcggcggc ctgctgaccg ccgtgctgat
    10501 ggtgtgcgtg gatgtgatga aagaaatgcc catcaccctg atgacgcgtc cgtttggctg
    10561 ggacaccctg gcggtgcgga tttttgaaat gacttccgag ggcgagtggg agcgcgccgc
    10621 gctgccggcg gtggccttgg tgctggtggg cctgctgccg gtgctgctgc tggcgcgcgc
    10681 gccctcgcac actggagcgc gcagatgact tcttctcctt tattgcagtt ggacgccatc
    10741 agccagcgct atgctcgcca cagcgtggtg gaacaattgt ccctgtcttt gcagcgcggc
    10801 gaaatcggct gcctgctggg cagctccggc tgcggcaaaa ccaccgtgct gcgctgcatt
    10861 gccggctttg agccgctcag cggcgggcgc atcctgattg atggccagct gctggccgac
    10921 gcccgcctgc acaccccggc gcacaagcgc cagatcggca tggtgtttca ggattatggt
    10981 ttgttcccgc acctgaccgt ggccggcaat gtcggctttg gcctggatgc gctggcgcgc
    11041 gacgcccgcg cggcgcaggt ggcgcgcatg ctggcgctgg ttggcctgga tggcctggcg
    11101 cagcgctacc cgcatgaatt gtccggcggc cagcagcagc gggtggcgct ggcgcgggcg
    11161 ctggccccgc agccgcgcct gttgctgctg gacgagccgt tttccaatct ggatgtgtcg
    11221 ctgcgcgagc ggctgggcca ggaagtgcgc gacatcctca aggcggcgca ggccacggcg
    11281 attttggtca cccacgacca gcacgaagcc ttcgccatgg ccgacaaggt gggggtgatg
    11341 gagcagggcc gcatcatgca gtgggacagc ccgtacaatc tgtaccacca gccggccaac
    11401 cgcatggtgg cggattttat cggccagggc gtgctgctgc ctggcgaagt gtgcgccgac
    11461 gcctgcatcc gcatcgagct gggcgaggcg tgcagcctgc gcccgcacgg ctttgccgcc
    11521 ggcagccggg tggaagtgct gctgcggccg gacgatgtgg cgcacgacga cgccagcccg
    11581 ctgcgcgcgc gcgtggcggc gcgggcgttt cgcggcgcag agtttctgta caccctggcg
    11641 ctgccctccg gccagaccgt gctgtcgctg gcccccagcc accacgacca cgccattggc
    11701 gaagaaatcg gcgtgcgtct ggatgccgaa cacctggtgg cgtttgccgt ctgagtttgc
    11761 cccatcagcc ttgcagcagc tgataagcca cggtcaacat caccagcgcc acgctgatgt
    11821 ccaacacccg ccaactggtc ggccgggtaa acagcggggc cagcaggcgc gcgccgtagg
    11881 ccagggcaaa aaaccacagc cacgaggcca gcgccgcgcc caggccaaag gtgaatcttt
    11941 cttgcgcggg cagttgcgcc gccaccccac ccagcagcac cacggtgtcc agatacacat
    12001 gcgggttcag ccaggtcacc gccagggtgg ccagcagcac tcgccacacc gattgctggc
    12061 tatccgccgg cagggtcatc gattcgccat gccaggcgcg gcgcaaggcc atggccgcgt
    12121 acaccagcaa aaaggccgcg ccgccccagc gcgccagcgc cagcaaggcc ggctggctgg
    12181 caatcaaggc cccggcgccg cccacgccca gggcaatcag caaggcatcc gaactggcgc
    12241 aagccagcgc aatctgcaag cgatgcgcgc cgcgcacccc ctgcgccagc acaaaggcgt
    12301 tttgcgcgcc aatcgccaca atcaagccgg cactgaccgc caacccctga aaaaataccg
    12361 cgctttgcat gtccattcct gtccgtctgt gcagaaaacc tgatggactc agcttacggg
    12421 ctggaataaa ttcagtaaaa ttgtttgttt ttcatcatca ttaaaaaaac ttaatcatgc
    12481 tcgacgccaa acccctggac gcgctggaag ccattgtccg cgccggcagt tttgagctgg
    12541 ccgcgcagtg gctgtgcatc accccatcgg cggtgtcgca gcgcattcgc cagctggaag
    12601 aacacctcgg gcaaaccgtg ctggtgcgca gccagccggt gcgccccacc ccggccggca
    12661 agcgcctgct gcgccatgtg cgccagctac aattgctgga ggctgagtta cgccatgatc
    12721 tggcgggtga tgtgcccgat gcctttacca cgctggcggt ggccgttaac gccgactcac
    12781 tggaaacctg gtttcctgaa gcgattgccg actgtgtggc gcaagaaaac ctgctgctgc
    12841 aattgattgt cgatgaccag gactacaccc acgccctgct gaaaaacggc gatgtgattg
    12901 gctgtgtgac gacgcaggcg caggcgctga ccggctgcca gagcgtgtgc ctggggcgca
    12961 tgccctatgt gtgcgtggcc acgccggcat ttgcccgccg ctattttgcc gatggcgtca
    13021 gcgccgaggc gctgcgcgcg gcgccggcga ttttgtttaa cctgaaagac agcctgcacc
    13081 tgaacttcat ggctgattac ggcctggcgc tgaatgattt tccgtatttc accgtgccgg
    13141 cggtgcatgc gctgctgaat gtggtgctgg ccgggctggg ctatgggctg acgccgcgtt
    13201 tacaagtggc cgacttgctg gccagcggcc gcctggtgga tgtgctggcc ggcgcgcccg
    13261 gcccggcgct gacactgtat tggcacagct gggcgctgca atcgcccaag ctggcgcgct
    13321 ttggcgcggc ggtgcaggcc ggcgcggcgc aggcgatgcc accagcatta tgaggcttgg
    13381 tcgggtgaga cgcgctgccg caggcagctg cgccagcaaa ccggactgac gtcgtcaatc
    13441 atactgacag ttgcgcaacc gcaaactata taaaagcgac acgatgtcaa ggctgcacgc
    13501 acgccaaggg cggcaagctt actggcgggt gccgcccatc ttgcggtaac ggccctggcc
    13561 atcgcct
//
LOCUS       DAOPYX010000040        13469 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_5038_length_13469_cov_8.630759, whole genome shotgun sequence.
ACCESSION   DAOPYX010000040 DAOPYX010000000
VERSION     DAOPYX010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 13469)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13469)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13469
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5038_length_13469_cov_8.630759"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     repeat_region   1..181
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=1..35
                     /rpt_unit_seq="cacccggtgggtcaccaccgggtgtggattgaaac"
     gene            300..581
                     /locus_tag="PKN55_03470"
     CDS             300..581
                     /locus_tag="PKN55_03470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HNX70338.1"
                     /translation="MPTATATARLEARISTDLHAMLKRAAELQGRTMTDFVVSAVQDA
                     AQRAIEQAEVIRLSLADQECFAQALLSPPKPAPALERAFTRRNTLLRTE"
     gene            578..1075
                     /locus_tag="PKN55_03475"
     CDS             578..1075
                     /locus_tag="PKN55_03475"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNX70339.1"
                     /translation="MSQAPFRLTPLDAAHDRAAFCCEAESLNRYLCKQASQDMRRRVA
                     ACFVALADDRRIAGYYTLASASLVLADLPASIGKKLPRYPSVPAVRLGRLAVDQAFKG
                     QGLGGALLADALARAARSEIAAFALVVDAKDEAAAAFYRHHGFIALPNSPLILFLPLA
                     TVPHA"
     gene            complement(1353..1733)
                     /locus_tag="PKN55_03480"
     CDS             complement(1353..1733)
                     /locus_tag="PKN55_03480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007342710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HNX70340.1"
                     /translation="MDIRPIRTEEDYQAALREVSAYFDNEPAPGTADGDRFEILLTLV
                     EAYEAKHYPIDLPDPVEAIKFRMDQAGLTPKDLTPVIGRLNRVYEVLNRKRPLTLNMI
                     WKLHDKLGIPAECLIQPQKMHRPA"
     gene            complement(1735..2040)
                     /locus_tag="PKN55_03485"
     CDS             complement(1735..2040)
                     /locus_tag="PKN55_03485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007342711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
                     /protein_id="HNX70341.1"
                     /translation="MNIIAVKFLRVFWEKHPDAEQALKSWVDEARKAAWTQPAEIKER
                     YRSASILKNRRVVFNIKGNDYRLVVSVAYHYQAIYVKFIGTHQEYDAIDAETVEMEV"
     gene            2811..4184
                     /gene="cysS"
                     /locus_tag="PKN55_03490"
     CDS             2811..4184
                     /gene="cysS"
                     /locus_tag="PKN55_03490"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014087932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HNX70342.1"
                     /translation="MLSLYNTLTRQKEVFTPIEPGKVRMYVCGMTVYDYCHLGHARVM
                     VVFDMVSRWLRASGLDVTYVRNITDIDDKIIKRAAENGESIQALTQRFIDAMNQDADA
                     LGIVRPTFEPRATEHISGMLALIAQLIANGKAYPAANGDVYYAVREFDGYGKLSGKSL
                     EDLRAGERVDVDPYKRDPLDFVLWKASKAGEPAWASPWGEGRPGWHIECSAMSCHHLG
                     EHFDIHGGGADLQFPHHENEIAQSEGAHGHAYVNYWMHNGFIRVDDEKMSKSLGNFFT
                     IREVLEKFDAEVVRFFILRAHYRSPLNYSDVHLADARNALTRLYTTLKNVPAAEVAID
                     WANPYAARFQAAMNDDFNTVEAMAVLFELAAESNRSGSAELAGLLKALGGVLGLLQRD
                     AQAFLQGGDDADGLSAEAIEALIAARRAARAAKNWAESDRIRDELTAAGIVLEDGAQG
                     TTWRRAG"
     gene            complement(4346..5707)
                     /locus_tag="PKN55_03495"
     CDS             complement(4346..5707)
                     /locus_tag="PKN55_03495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011133938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNX70343.1"
                     /translation="MPFSRLGLSDPIVRAVTELGYTTPTPIQTQAIPAVLSGGDLLAG
                     AQTGTGKTAGFTLPILQRLSSTPPANPQARPGVRALILTPTRELAAQVEENVRGYARY
                     LKLTSMVMFGGVGMNPQIEQLKKRVDILVACPGRLLDHLSQGSLDLNSVEILVLDEAD
                     RMLDMGFIHDIKKVLAKLPPKRQNLLFSATFSEDIRGLADRLLDRPALIEVAPRNATA
                     DRVRQQVYRVDRERKRDLLSHLIQQNNWQQVLVFTRTKHGANRLAEKLEKDGIPSAAI
                     HGNKSQSARTRALAGFKSGELRVLVATDIAARGIDISELPHVVNFELPNVPEDYVHRI
                     GRTGRAGAEGEAVSLVCVDENKLLVDIEKLIRRPLPCSQLPGFEVDPSIAAEPIPNGR
                     NAPRGGGGGRGAPRGGSQPAGKPGQGGKPQGARQGQGGPRNGGNGGNGQARAPKPSTS
                     RHH"
     gene            6206..6874
                     /locus_tag="PKN55_03500"
     CDS             6206..6874
                     /locus_tag="PKN55_03500"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: protein motif:HMM:NF009695.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008967 - phosphoglycolate phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HNX70344.1"
                     /translation="MFSTPIRAIAFDLDGTLVDSLTDIAAAANAARAELGLAPLADSR
                     VRSFIGDGSAMLMARTAQDDPHAQVDDSPLQIAARAHFNQHYARVVAEHTRPYPGAVE
                     GLQALAALGLPLACVTNKPIAFTTPLLAALDLERYFELVLGGDSLAAKKPDPLPLAHV
                     AQALAVAPAQLLMVGDSKNDVLAARAHGSPVLAVSYGYTASASALGADAVIDRIDQIV
                     ALLG"
     gene            6972..7160
                     /locus_tag="PKN55_03505"
     CDS             6972..7160
                     /locus_tag="PKN55_03505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017244801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HNX70345.1"
                     /translation="MTCNIGKTERILRVIVGLIITSLAFVGPQSPWAYLGLVPVLTGL
                     LGWCPPYAMLGINTCKKK"
     gene            complement(7375..10545)
                     /locus_tag="PKN55_03510"
     CDS             complement(7375..10545)
                     /locus_tag="PKN55_03510"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNX70346.1"
                     /translation="MNLPPAARAVLHALACVPDGLSERALLMVLPPLLPADAPPLPPR
                     PQAILRRLTEQGWLTARPGGWRLAEARLGEVWRDIAELPWLWPLLAMLMEKLYDVPPS
                     PAQAHRQDWLARLQAGDRSFLPGLSAAHAALAQLNAKADAAQPAAPSVERRLAWRLEA
                     SDGGVELEAREQKRTAQGWTPGRVLSWKRLLGELPDWLSAQDHAALRCAKVSEVYYYG
                     GESYTLDEDALPALVGHPALFAAEPPHARIELSLGQPALVLRQQGDMLRLEVSPPQLT
                     DARTQWLDHSQPEHWRLYRLDGELKRLVALVGHGLAVPRAARAPLLDAISQLAPLLPI
                     HSDLTDLAGQLPALPADATLYALLRADGDSLRVQLRVRPLPGGRDYRPGQGMVTVVGA
                     VDGEAVHTCRDLDAERRAHAAVLRQCPTLAACPHDPIIGERVCVSIDQALDVLAELQA
                     VGEAQLRCVWPAGQRLRLRGPAHAGQARLSIRQRGDWFAVNGELALEDDGRLLQLREL
                     LTLVGQHSGRYVRLGEADWLALDDALRQQLAALARASAGLDADGAAQLSRLAAPMLAQ
                     LADSAAACEGDAGWTAQRARWQALEQISPAVPSALRATLRDYQLAGFHWLARLAHWGV
                     GACLADDMGLGKTMQTLALCLLRAEHGPQLVVAPTSVAQNWLAEAARFAPSLRLRPYA
                     QQRALDALAPGDLVVVSYGLFQLDAERFAQVHWASVILDEAQAIKNPDTRRAQAAFAL
                     RADFRLAASGTPVENHLGELWSLFRFLNPGLLGSLEQFGERFATPIAEGDEPARLALR
                     RLLQPFILRRTKTQVLTELPPRTELTYRVELSDGERELYEALRQEALEQVAQAPEHER
                     AMQVLAHITRLRRLCCHPTLALPNATLPGSKLAAFARLMRELLDNGHKALVFSQFVDH
                     LALARRWLDEQGIAYHYLDGATPARQRQKKVEAFQGGQGEVFLISLKAGGSGLTLTAA
                     DYVLHLDPWWNPAVEDQASDRAYRMGQQRPVTIYRLVAAHTIEERILALHAAKRELAD
                     ALLAGGDASARLDTDALLALLREAAS"
     gene            complement(10736..12106)
                     /gene="ccoG"
                     /locus_tag="PKN55_03515"
     CDS             complement(10736..12106)
                     /gene="ccoG"
                     /locus_tag="PKN55_03515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014085545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase accessory protein CcoG"
                     /protein_id="HNX70347.1"
                     /translation="MTVATLRRPATGGPCVPKLTSGRFNTWRVAMVLLTQLIFLGAPW
                     LRWDGHQAIWLDLEGQRIFLFAWCFWPQDLVYLAGALIICALGLFWWTALAGRLWCGN
                     SCPQTVYTEIMLWIEHWIEGDRPARLKLRADPYKPAHLARRAASQAVMIAVSLVIGFG
                     LVAYFVPAPELLASVLDGSMGGWTLFWLLSYAGFTYLLAGRLREQVCLHMCPYARFQS
                     VMFDSHTLLVGYDAKRGEPRGARRQQQTANPRGDCVDCGVCVQVCPTGIDIRNGLQYE
                     CIGCGACIDACDTVMDKLRAPRGLIRMTTPHAEASGQPARVSWLRPRPLIYLGLIAAT
                     ALAMAVGLWQRAPLHVNVLRDRAVMVRETPEGLLENAYQIKVFNADSRPHVLQLSAEG
                     LPGARVLTEPAQLLLPAHGQLQLLARVQAAPDNVQHGAQVLHLNVRSRDDASFQRQED
                     TVFIGE"
     gene            12526..13284
                     /locus_tag="PKN55_03520"
     CDS             12526..13284
                     /locus_tag="PKN55_03520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX70348.1"
                     /translation="MFIPPVSPQLPVYQPKAPDMVPDVTAVGRTSPVQAETLGRSVQQ
                     DAQRASERRPLRPRKPNDWDAVDVSERGELLLLLDDVAEELLAVRSRLASDEAVYTVL
                     RAADQLRQAYQRLAAPYGRHDNPADLLGVAASLQEGLTHVPAQDDGLPMSSLGVRLGA
                     EGELHVDAARLAWACQQRPDMARRLDAVVANLHAQVLGAQRRLNHPATGPGRGLDAQL
                     LSAHYAAQSAQVMASASHPPRRMPPTIHDVVEKP"
     gene            13384..13459
                     /locus_tag="PKN55_03525"
     tRNA            13384..13459
                     /locus_tag="PKN55_03525"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13417..13419,aa:Asn,seq:gtt)
BASE COUNT         2407 a         4300 c         4385 g         2377 t
ORIGIN      
        1 cacccggtgg gtcaccaccg ggtgtggatt gaaactgccg cctgcgccgc catcgcaatg
       61 tttgggcttt cgtcacccgg tgggtcacca ccgggtgtgg attgaaacac tcgcaaattt
      121 gtgcagcgtg cccgcgaggc agggtcaccc ggtgggtcac caccgggtgt ggattgaaac
      181 ccgtgacggg ctgcccgata tgtggcatgg cgcagactga acaagcaacc ataaagccgc
      241 aagacctgcc ttgagctgta cggcaatttg ccgtattgtt gaatcaggag gataaaacca
      301 tgcccactgc caccgcaacc gctcgtctcg aggcccgcat cagcaccgat ctgcatgcga
      361 tgctcaagcg cgcagctgaa cttcaggggc gcaccatgac tgattttgtg gtgtccgccg
      421 tgcaagacgc ggcgcaacgc gccattgagc aagccgaagt gatccggctg tctctggccg
      481 atcaggaatg ttttgcgcaa gcgctgttgt cgccacccaa accggcgccc gccttggagc
      541 gcgcctttac ccggcgcaac acgctgttgc gcacggaatg agccaggcgc cgtttcggct
      601 gacgccgctg gatgccgcac atgatcgcgc cgctttttgc tgcgaggcgg agtcgctgaa
      661 ccgctatctg tgcaagcaag ccagccaaga catgcgccgc cgtgtggcgg cttgttttgt
      721 ggccctggcg gatgatcggc gcattgccgg ctactacaca ttggcctcgg caagcctggt
      781 gctggccgat ctgccggcca gcattggcaa aaaactgccg cgctatccaa gcgtgcccgc
      841 cgtccgcctg ggccgcctgg cggtcgatca ggcgtttaag gggcaaggtt tgggaggggc
      901 gctgctggcc gatgcgctgg cgcgcgctgc ccgttcagaa attgccgcct ttgccctggt
      961 ggtggatgcc aaggatgagg ccgccgccgc gttctaccgg catcacggct ttattgcctt
     1021 gccaaactca ccgcttatcc tgtttttgcc gctggccacc gtcccgcacg cttgacgcac
     1081 cattgaaacg tccagccgcg attgagctgg gcggcaggcc agaggtcacc cggtgggtca
     1141 ccaccgggtg tggattaaga gcggctaaca aaaccctcct ggcgttgttg cacgaccttg
     1201 ccgtactacc tgtactgtct acggtcgtgc gcctagccag gatcgcttcg ctgggttttg
     1261 ttagccgctc taaaacgaaa attacacccg caccgcgcaa atcatgcgca tcacccggca
     1321 gatcgttacc cgttatggat gaagcacaaa gatcaggccg ggcggtgcat cttctggggc
     1381 tgtatcaggc actcggccgg aatgcccaac ttatcgtgta gcttccagat catgttcagc
     1441 gtcagcgggc gtttgcggtt cagaacctca tagacgcgat tcagccgacc gatgacaggc
     1501 gttaagtcct tgggcgtcag ccctgcttga tccatgcgga atttgatggc ctcgaccggg
     1561 tcgggcaggt caatcgggta gtgtttggct tcgtaggctt ccaccagcgt cagcaggatt
     1621 tcaaaacggt caccgtctgc cgtgccaggg gccggttcat tgtcgaagta ggccgagact
     1681 tcacgtagcg cggcctgata gtcctcttcg gtgcggatcg ggcgaatatc catgtcagac
     1741 ctccatttct acagtttcgg cgtctatggc atcgtattcc tgatgggtgc cgatgaattt
     1801 cacatagatc gcttggtagt ggtaagcaac agacaccacc agtcggtaat cgttgccctt
     1861 gatgttgaac accacgcggc gatttttgag gatgcttgcg cttcgatagc gttctttgat
     1921 ctcagcgggt tgtgtccatg cggccttcct ggcttcatct acccaagact tgagcgcttg
     1981 ttctgcatcc gggtgttttt cccagaaaac gcgaagaaat ttaacggcga tgatgttcat
     2041 cgtggcgatt atagtcccac cttgggacaa aagaaatgac aagcaagaca acacactatc
     2101 cggcaacacg atggccacct ggtggggcgt gcaaggattt gatgctcgct cggcaggcgc
     2161 ccggcgggtc acatcagtga caatttgacg gcccactgat tgtctgatcg ggcattgacc
     2221 cgcccgcacg ccctgcgcac aatgcccaca cccaccaggc caccccaccg ccaaagctcg
     2281 ccccgcgcga accccaagcg aacggccaaa cccggtgggg ttcgcgctgg agtgagtcca
     2341 ttgaatataa aactcttttt tctcgatgct cgggggtatg cccgctcacc atccaatgtg
     2401 ggcggcaggt tcgcgcgcga cgcagaaaaa tgggtggaaa ttcaaggggt ttctggcgag
     2461 aagtcacccg gtgggtcacc accgggtgtg gattgaaact gcgcgggacc tgcgcaacag
     2521 ctcgcagttc gagcgtcacc cggtgtgtca ccaccgggtg tggattgaaa ctaccgtctg
     2581 gtggcgggtg gggtggagta cggctgtcac ccggtgggtc accaccgggt atagcttgaa
     2641 accacatgca ggaagcccct gaaacatccc tgccgtagtc acccgccttc tcaccaaata
     2701 ccggttaaag cgcacgttcg ctaaagccat cacggcagcg ctcggcccgt gcttgggctg
     2761 ggcgatgcta caatgctggt tttacgcatt cgcccgaatc aggactgtcc atgctctcgc
     2821 tttacaacac gctcacccgc cagaaagagg tgttcacccc catcgaaccc ggcaaggtcc
     2881 gcatgtatgt gtgtggaatg acggtgtacg actactgcca tctgggtcat gctcgggtga
     2941 tggtggtgtt tgacatggtc agccgctggc tgcgcgccag cgggctggac gtgacttatg
     3001 tgcgcaatat caccgatatt gacgacaaaa tcatcaagcg cgcggctgaa aacggtgaat
     3061 ccatccaggc gctgacccag cgctttatcg acgcgatgaa ccaggacgcc gacgcgctgg
     3121 gcattgtgcg ccccaccttc gagccgcgcg ccaccgagca tatctccggc atgctggcgc
     3181 tgattgccca gctgatcgcc aacggcaagg cctacccggc ggccaatggc gatgtgtatt
     3241 acgccgtgcg cgagtttgac ggctacggca agttgtcggg caaatcgctg gaagacctgc
     3301 gcgcgggcga gcgggtggat gtggatccat acaagcgtga tccgctggac tttgtgctgt
     3361 ggaaggcctc gaaagccggc gagccggcct gggcgtcgcc gtggggcgag ggccggccgg
     3421 gctggcatat cgagtgctcg gcgatgagct gccaccacct gggcgagcat ttcgacatcc
     3481 acggcggcgg cgcggatttg cagtttccgc accacgaaaa cgaaatcgcc caaagcgagg
     3541 gcgcgcacgg ccacgcctat gtgaattact ggatgcacaa tggctttatc cgcgtggacg
     3601 acgaaaaaat gtccaaatcg ctgggcaatt tcttcaccat ccgcgaggtg ctggaaaaat
     3661 tcgacgccga agtggtgcgc ttttttattc tgcgcgccca ctatcgcagc ccgctgaatt
     3721 attctgacgt gcatctggcc gacgcccgca atgcgctgac ccgcttgtac accacgctga
     3781 aaaacgtgcc ggcggccgag gtggccatcg actgggccaa cccgtatgcc gcgcgttttc
     3841 aggcggcgat gaacgacgat ttcaacaccg tcgaagccat ggccgtgctg tttgagctgg
     3901 cggcagaaag caaccgcagc ggctcggccg agctggctgg cctgctgaag gcgctgggcg
     3961 gcgtgctggg cttgttgcag cgcgatgcgc aggcgttttt gcagggcggc gatgacgccg
     4021 atgggctgtc agccgaggcc atcgaggcgc tgattgccgc ccgccgcgcc gcacgcgccg
     4081 ccaaaaactg ggcagaaagc gaccgcatcc gcgacgagct gaccgccgcc ggcatcgtgc
     4141 tggaagatgg cgcgcagggc acgacctggc gccgcgccgg ctgatacccc gtcagccgcg
     4201 ataaaaaacc ccgcgacatc aggccgatgt cgcggggttt ttttagcgct gccaacaaaa
     4261 ctcagcgaag cggtgctggc cagatgccgt acatgtggta cggcaaggtc gggcaacgac
     4321 gccaggaggg ttttgtgaac cgcgcttagt gatggcgcga ggtggacggc ttgggcgcgc
     4381 gcgcctggcc attaccacca ttaccgccat tgcgcgggcc gccttggccc tggcgggcgc
     4441 cttgcggctt gccgccctgg ccgggcttgc cggccggctg gctgccgccg cgaggcgcgc
     4501 cgcgcccacc gccgccgccg cgcggggcgt tgcggccatt gggaatcggc tcggcggcaa
     4561 tgctcgggtc cacttcaaaa cccggcagct gggagcacgg cagcggacgg cggatcagtt
     4621 tttcgatatc caccagcagc ttgttttcgt ccacgcacac cagcgacacc gcctcgccct
     4681 cggcgccggc gcggccggtg cggccaatgc ggtgcacgta gtcttccggc acattgggca
     4741 actcgaagtt gaccacgtgc ggcagctcgc tgatgtcgat gccgcgcgcg gcgatgtcgg
     4801 tggccaccag cacgcgcaat tcgccgctct tgaagccagc cagcgcgcgg gtgcgggccg
     4861 attggctctt gttgccgtga atggcggcgg acgggatgcc gtctttttcc agcttttctg
     4921 ccaggcggtt ggcgccgtgc ttggtgcggg tgaacaccag cacttgttgc cagttatttt
     4981 gctggatcag gtgcgacagc aaatcgcgct tgcgttcgcg gtccacgcgg tacacttgct
     5041 gacgcacgcg gtcggcggtg gcgttgcgcg gggcgacttc gatcagcgcc gggcggtcca
     5101 gcagccggtc ggccaggccg cggatgtcct cagagaaggt ggccgagaac agcaggtttt
     5161 gccgtttggg cggcagcttg gccagcacct ttttgatgtc gtggataaag cccatgtcca
     5221 gcatgcggtc ggcttcgtcc agcaccagaa tttcgacgct gttcaaatcc agcgagcctt
     5281 gcgacagatg atccagcagg cggccagggc aggccaccag gatatccacg cgctttttca
     5341 gctgctcgat ttgcgggttc atgcccacgc cgccaaacat caccattgag gtgagcttga
     5401 gataacgggc gtaaccgcgc acgttttctt ctacctgcgc cgccagctcg cgggtggggg
     5461 tgaggatcag cgcgcgcacg ccggggcgcg cctgcgggtt ggccggcggg gtgctggaca
     5521 aacgctgcaa gatgggcagg gtgaagccgg cagtcttgcc ggtgccggtc tgcgcgcccg
     5581 ccagaagatc accaccgctc aacacggcag ggatggcctg ggtctggatc ggggtggggg
     5641 tggtgtagcc gagctcagtg acagcgcgga cgatggggtc ggacaacccc agtcgggaaa
     5701 aaggcatgca aacaactcca gccgccgcgc atctgcgcgg acgggagata aaaccagggc
     5761 gggaaccttg ccggggaacg cgcacgcgcc gtccgtcaca cgccgccacg ccggaccaac
     5821 aaacgcggcc agcgccccgt gcatccattc agttcacagc tttgaatgcg gcgcgcctgg
     5881 atgtggggct gacgtaggct tgcgccggac aaacgcgagg cgcttgtgtc agtctgtgtc
     5941 aggctatatc agccacagaa aaagcgaatg gttcgcgatt atagcgaatc gcacacaaac
     6001 taggtgtaaa tactgggttt ctgtgctgcg tacgtcgtac acaaagcggg gcatcttaac
     6061 attgccgggc gggaaatggt ctgaccaagc accgtatttt tacggattta tgatctacgt
     6121 cagattttca ccacaccgta tccgcttgcc ttgaccatgg acgggcaggc accatgctgt
     6181 tttgtttgcc accggaatgt ttgccatgtt cagcaccccg attcgcgcca tcgcctttga
     6241 cctggatggc accctggttg acagcctgac cgacatcgcc gccgccgcca acgctgcccg
     6301 cgccgagctg ggcctggcgc cgctggccga tagccgggtg cgcagcttta ttggcgatgg
     6361 ctcggccatg ctgatggcgc gcaccgccca ggacgaccca catgcccagg tagatgattc
     6421 cccgctgcaa atcgccgccc gcgcgcactt caaccagcat tacgcccgcg tggtggccga
     6481 gcacacccgc ccctatcccg gcgcggtgga aggcttgcag gcgctggcag cgctgggcct
     6541 gccgctggcc tgcgtgacca ataagccgat tgcttttacc accccgctgc tggcagcgct
     6601 ggatctggag cggtatttcg agctggtgct gggcggcgac agcctggccg ccaaaaaacc
     6661 cgatccgctg ccgctggcgc atgtcgccca ggccctggcc gtcgccccgg cacagctatt
     6721 aatggtgggc gactcaaaaa acgacgtgct ggccgcccgc gcgcacggct cgccggtgct
     6781 ggcggtgagc tatggctata ccgcatcggc cagcgcactg ggcgcagatg cagtgattga
     6841 ccgcatcgac cagattgtgg cgctgttggg gtgagctggg cgggcatgcc tgccggacag
     6901 ggctttcctt tcagcacaat ttgttatatg tttatataaa ttgttcaagt tcagcccaag
     6961 gagcccttac catgacctgc aatatcggca aaaccgagcg cattctgcgc gtgatcgtcg
     7021 gcctgatcat caccagcctg gcgtttgtcg gcccgcaaag cccgtgggcg tatctgggcc
     7081 tggtgccggt gctgaccggc ctgctgggct ggtgcccgcc gtatgcgatg ctgggcatca
     7141 atacctgcaa gaaaaagtaa gctgccgaaa gctcattcca gacgggacgt catcccggcc
     7201 ctctgacgaa agccccaccc ccgttgctcg ggcgtggggc tttgtctgtt tagcgggact
     7261 tcgagcggct cacaaaaccc agcgcagcgg tcctggctag gcgtgcggcc gcagacagta
     7321 caagcagtac ggccaggtcg cgcaacgacg ccaggagggt tttgtgagcc gcgcttagct
     7381 cgccgcttcg cgcagcaggg ccaacagagc atcggtgtcc aggcgggcgc tggcgtcgcc
     7441 gccggccagc agggcgtcgg ccagttcgcg tttggcggcg tgcagggcga ggatgcgttc
     7501 ttcgatggtg tgggcggcca ccagccggta gatggtcacc gggcgttgct ggcccatgcg
     7561 gtaggcgcgg tcgctggcct ggtcttctac tgccgggttc caccaggggt ccagatgcag
     7621 cacatagtcg gcggcggtca gcgtcaggcc cgagccgccg gctttcaggc tgatgagaaa
     7681 cacctcgccc tgaccgccct gaaacgcttc gacttttttc tggcgctggc gcgccggggt
     7741 ggcgccgtcc aggtagtggt aggcaatgcc ttgttcgtcc agccagcggc gcgccagcgc
     7801 caggtgatcg acaaactggc tgaacaccag cgccttgtgg ccgttgtcca gcagctcgcg
     7861 catcagccgg gcaaaggcgg ccagcttgct gccgggcagg gtggcgttgg gcagggccag
     7921 ggtggggtgg cagcacagcc ggcgcaggcg ggtgatgtgc gccagcacct gcatggcgcg
     7981 ctcgtgctcg ggcgcttgcg ccacttgctc cagcgcctcc tggcgcaggg cttcgtacag
     8041 ctcgcgctcg ccgtcggaca gctcgacccg ataggtcagc tcggtgcgcg gcggcagttc
     8101 ggtcagcacc tgggttttgg tgcggcgcaa gataaagggt tgcagcaggc ggcgcagcgc
     8161 caggcgggcg ggctcgtcgc cctcggcaat tggcgtggca aagcgctcgc caaattgctc
     8221 caggctgccc agcaagcccg gattgagaaa acgaaacaag ctccacagct cgcccaaatg
     8281 gttttccacg ggcgtgccgc tggcggccag ccggaaatcg gcgcgcaggg caaacgccgc
     8341 ctgggcgcgg cgggtgtccg ggtttttgat ggcctgggct tcgtccagaa tcacgctggc
     8401 ccaatgcacc tgggcaaagc gttcggcgtc cagctgaaac aagccatagc tgaccaccac
     8461 cagatccccc ggcgccagcg cgtccagcgc gcgttgctgg gcatacggcc gcaggcgcag
     8521 gctgggggca aagcgggcgg cttcggccag ccagttttgc gccaccgagg tgggggcgac
     8581 caccagttgc gggccgtgtt cggcgcgcag caggcacagc gccagggttt gcatggtttt
     8641 gcccagaccc atatcgtcgg ccagacaagc gcccacgccc cagtgcgcca gccgcgccag
     8701 ccaatgaaaa ccggccagct gataatcgcg cagcgtggcg cgcaaggcgc tgggcacggc
     8761 ggggctgatc tgttccagcg cttgccagcg cgcgcgctgc gcggtccagc cggcgtcgcc
     8821 ttcacaagcg gcggcgctgt cggccagttg cgccagcatc ggcgcggcca gccggctgag
     8881 ctgggcagcg ccgtcggcgt ccagcccggc gctggcgcgg gccagcgcgg ccaattgctg
     8941 acgcagcgcg tcgtccagcg ccagccagtc ggcttcgccc aagcgcacat agcggccgct
     9001 gtgctggccg accagggtta acagttcgcg cagttgcagc aggcggccgt cgtcttccag
     9061 cgccagctcg ccgttgacgg caaaccagtc gccgcgctgg cgaatgctca gccgcgcctg
     9121 gccggcgtgc gccgggccgc gcaggcgcaa gcgctggcct gccggccaca cgcaacgcag
     9181 ctgcgcctcg cccaccgctt gcagctcagc cagcacgtcc aaggcttgat caatgctgac
     9241 gcacacccgc tcgccgatga tggggtcgtg cgggcaggcg gccagtgtcg ggcattggcg
     9301 cagcacggcg gcgtgggcgc ggcgttcggc gtccaggtcg cgacaggtgt gcaccgcctc
     9361 gccgtccacc gcgcccacca ccgtcaccat gccctggccg gggcggtagt cgcgcccgcc
     9421 aggcagcggg cgcacgcgca gttgcacgcg caggctgtcg ccgtcggcgc gcagcagcgc
     9481 atacagcgtg gcgtcggccg gcagcgccgg cagttggccg gccaggtcgg tgaggtcgct
     9541 gtgaatcggc aacagcgggg ccaactggct gatggcgtcc agcaggggcg cgcgcgccgc
     9601 gcgcggcacc gccaggccat ggccgaccag cgccaccagc cgcttgagct cgccatccag
     9661 ccgatacagc cgccagtgtt cgggctggct gtggtccagc cactgcgtgc gggcgtcagt
     9721 cagctgtggc ggcgagactt ccagccgcag catatcgccc tgctggcgca gcaccagcgc
     9781 cggctgcccc aggctaagct cgatgcgcgc gtgcggcggc tcggcggcaa acagcgccgg
     9841 gtggcccacc agcgccggca gggcgtcttc gtccagggtg tagctttcgc cgccgtagta
     9901 atacacctcg ctgaccttgg cgcagcgcag cgcggcgtgg tcttgcgcgc tcagccagtc
     9961 gggcagctca cccagcaggc gcttccagct cagcacacgg ccgggggtcc agccctgggc
    10021 ggtgcgtttt tgttcgcgcg cttccagctc gacgccgccg tcgctggctt ccagccgcca
    10081 ggccagccgg cgctcgacgc tgggggcggc cggctgggcg gcgtcggcct tggcgttcag
    10141 ttgcgccagc gcggcgtggg cggcgctcag ccccggcaaa aaactgcggt cgccggcttg
    10201 cagccgggcc agccagtctt gccggtgcgc ctgcgccggg ctgggcggca cgtcgtacag
    10261 tttttccatc agcatggcca gcagcggcca tagccagggc agctcggcga tgtcgcgcca
    10321 cacctcgccc agccgcgcct cggccagccg ccagccgccg gggcgggcgg tcagccagcc
    10381 ttgctcagtc agccggcgca agatggcttg cgggcgcggc ggcaagggtg gggcgtcggc
    10441 cggcagcaag ggcggcagca ccatcaataa agcgcgttcg gacaggccat cgggcacaca
    10501 ggccagcgcg tgcagcacgg cgcgggcggc ggggggaagg ttcatggcgg gcaagtacaa
    10561 ggtgcaaacg gggaaggccc ggattatagc ctgtggcccg acgtggcggc gcccacccgc
    10621 tgggctgggt gggcgtggct gcgtgtgatt tgtcagcgag ccgagcggct caccactctc
    10681 agcgcagcaa atctggtcag gcgcacgacc gcgacaagca tggggtgagc ggcgcttatt
    10741 cgccgataaa caccgtgtct tcttggcgct ggaagctggc atcgtcccga ctgcgcacat
    10801 tcaagtgcag tacctgtgca ccatgctgca cattgtccgg cgcggcttgc actcgcgcca
    10861 gcaattgcag ctggccatgc gccggcaaca gcaattgcgc cggttcggtc agcacccgcg
    10921 cgccaggcag gccttcggcg ctcagttgca agacatgcgg gcggctgtcg gcgttgaaca
    10981 ctttgatctg gtaggcgttt tccagcaggc cttccggggt ttcccgcacc atcaccgcgc
    11041 ggtcgcgcag cacattcaca tgcagcggcg cacgctgcca caggcccacc gccatggcca
    11101 gcgccgtggc ggcaatcagg cccagataaa tcaagggacg cggacgcagc cagcttaccc
    11161 gcgccggctg gccgctggcc tcggcgtgcg gggtggtcat ccggatcagc ccgcgcggcg
    11221 cgcgcagctt gtccatcacc gtgtcgcagg cgtcgatgca ggcgccacag ccgatgcatt
    11281 cgtattgcag gccgttgcga atatcaatgc cggtggggca cacctgcacg cacacgccgc
    11341 agtccacgca gtcgccgcgc ggattggcgg tttgctgctg gcggcgcgcg ccgcgcggct
    11401 cgccgcgctt ggcgtcgtag cccaccaata aggtgtgcga atcaaacatc acactctgaa
    11461 aacgcgcata cgggcacata tgcaggcaca cctgctcgcg cagccggccg gccagcagat
    11521 aggtaaagcc ggcgtaagac agcagccaga acagcgtcca gccgcccatg ctgccgtcca
    11581 gcacactggc caacagctcg ggcgccggca caaaatacgc caccagacca aagccaatca
    11641 ccaggctgac ggcaatcatc accgcctggc ttgccgcgcg ccgcgccaga tgcgccggtt
    11701 tatacgggtc ggcgcgcaat ttcagccgcg ccgggcggtc gccctcgatc cagtgttcga
    11761 tccacagcat gatttcggtg tacaccgttt gcgggcagct attgccgcac cacagccggc
    11821 cggccagcgc cgtccaccag aacagcccca gcgcacagat aatcagcgcg ccagccagat
    11881 acaccaaatc ttgcggccag aaacaccagg caaacaaaaa aatccgctgg ccttccagat
    11941 ccagccagat ggcctggtgg ccatcccagc gcagccaggg cgcgcccaaa aaaatcagct
    12001 gggtgagcaa caccatggcc acccgccagg tgttgaagcg cccagatgtg agcttgggca
    12061 cacaggggcc gccggtggca gggcggcgca gggtggcaac agtcatgtta ttctccggca
    12121 acaatgaatg gccgcagggt aacgcgccgg ttctgtgccc tgccagacac agaaaaacct
    12181 gattttgcag agcacagaaa acccgtgtat ccagcgcggg gcggcgcatc gactttctgt
    12241 tgataaatca tcaagttgca tgacaattac attgcatgca tactgatggc tgtacactct
    12301 gggacgcgcg ggaacttttc tgagcctggg cccgccgtac tctgcaacac ggcgaataaa
    12361 cgccgctcac aatacccagc gcagcgcgcc tggctaggcg cacggccggt acacgcaata
    12421 cggcaaggtt gcggcacaat cccagacagg ttttgtcagc agcgccaaaa cagcgcacgg
    12481 catgcgccaa gcccagtgtc cgaggtcagc aaggagtgcg tcatcatgtt catccccccg
    12541 gtttctcccc agctcccggt gtaccagccg aaagcgccgg atatggtgcc ggacgtcacc
    12601 gccgtgggcc gcacctcgcc cgtacaggca gaaacgcttg gccgttcggt gcagcaagat
    12661 gcccagcgcg ccagcgagcg tcggccactg cggccgcgca agcccaacga ctgggacgcg
    12721 gtggatgtgt ctgaacgcgg cgaactatta ctcttgctgg atgatgtggc cgaagaactt
    12781 ctggcggtgc gcagccggct ggccagcgat gaggcggtgt acaccgtgct gcgcgccgcc
    12841 gatcaattgc gccaggctta tcagcgcctg gccgcgccgt atggccggca tgacaacccg
    12901 gccgatttgc tgggcgtggc agccagcttg caggaagggc tgacgcatgt gccggcacaa
    12961 gacgatggcc tgccaatgag cagcctgggc gtgcggctgg gggctgaagg tgagctgcat
    13021 gtggatgccg cccggctggc ctgggcctgc cagcagcgcc cggacatggc gcgccggctg
    13081 gatgccgtgg tggccaatct gcatgcccag gtgctgggcg cccaacgccg gctgaaccac
    13141 ccggctaccg gcccggggcg cggtctggat gcgcaattac tgtcggcgca ttatgcagcc
    13201 cagagtgccc aggtgatggc cagtgccagc catccaccgc gccgcatgcc gccgaccata
    13261 catgatgttg ttgaaaaacc atgatttttt caaaaaattt acgaataacg gcattttttt
    13321 cacattaact gttgacaagc atggggcgca tgcgtatgat gcgctccatc gattcaattc
    13381 gattcctcga tagctcagtc ggtagagcga cggactgtta atccgcaggt ccctggttcg
    13441 agcccaggtc gaggagccag attcaaaaa
//
LOCUS       DAOPYX010000041        13260 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_5150_length_13260_cov_8.487997, whole genome shotgun sequence.
ACCESSION   DAOPYX010000041 DAOPYX010000000
VERSION     DAOPYX010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 13260)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13260)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13260
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5150_length_13260_cov_8.487997"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..404
                     /gene="fliP"
                     /locus_tag="PKN55_03530"
     CDS             <1..404
                     /gene="fliP"
                     /locus_tag="PKN55_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012697819.1"
                     /note="FliP, with proteins FliQ and FliR, forms the core
                     of the central channel in the flagella export apparatus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliP"
                     /protein_id="HNX70349.1"
                     /translation="VPFSEDKINFLQALDKGSVPMKQFMMRQTREKDLEFFIEVSQSA
                     KPRGPEDISMKTLAPAFIISEMKTAFQIGFMIFIPFLIIDLVVASILMAMGMMMVSPV
                     TIALPFKLMLFVLVDGWTLLLGSLVQSFYVS"
     gene            416..685
                     /gene="fliQ"
                     /locus_tag="PKN55_03535"
     CDS             416..685
                     /gene="fliQ"
                     /locus_tag="PKN55_03535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HNX70350.1"
                     /translation="MTPEFVINLVQNALFMLIVVSAPIMAVALLVGLLVSVLQAATQI
                     NEMTLTFIPKLLAMFVVLVVAGPWMINTLMDYTIRLFTSIPNAVG"
     gene            701..1501
                     /gene="fliR"
                     /locus_tag="PKN55_03540"
     CDS             701..1501
                     /gene="fliR"
                     /locus_tag="PKN55_03540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HNX70351.1"
                     /translation="MFPAALHLSDAQISALLSQYLWPLFRILGFFMAEPLFSSRSIPR
                     RAKVIMGIMLTIMISPLLPPMPVIPVISGAGLLVLMHQVLIGLAMGYVMRLMLTAVEM
                     AGFFISSQMGLGFAMFFDPQHSGQVPTISRLMSLLVLLLLMAMNGHHLMLSTLIDSFK
                     VFPITADGLPLISYRLLAEWGAEVFSLGLLLSMPVMAALLIANVAMGIMTRAAPQFNV
                     FSFGFPLTITIGFVVLYFMMPYFIAATQMLYQHGTELVLTLIKAKAEP"
     gene            complement(1519..2397)
                     /locus_tag="PKN55_03545"
     CDS             complement(1519..2397)
                     /locus_tag="PKN55_03545"
                     /inference="COORDINATES: protein motif:HMM:NF020481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1853 family protein"
                     /protein_id="HNX70352.1"
                     /translation="MTASADFQHAVVRDLAELLLGAAPWHTGCEIPRERLLGAHGLAL
                     LHQLDRAPAPLLAILAQRPASRRLGRYVEALLWAWCQLAPHCSELHANLPVRDGGRTL
                     GEFDLLLRLDGEPWHVEVACKYYLVVGRQAVGVDLHDAWLRKFAKLEQQLRLSRHPLA
                     QAVLPAGWQNCQVGSLLRGRFFHRDAPVTPAPLNPLAPAGWWRPLAEPWPGEQDASVR
                     YMHLPRLRWLGSACGGQTGGDSAASLRAQLADYDSPQQIAVLQQNGEGVWQEAGRGFI
                     VPGSWLESDMLTLAEA"
     gene            complement(2394..3146)
                     /locus_tag="PKN55_03550"
     CDS             complement(2394..3146)
                     /locus_tag="PKN55_03550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011137192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HNX70353.1"
                     /translation="MMKPEAASALQNIRVVLARPSHPGNIGSAARAMKTMGLTRLTLV
                     APREFPSTEAVTLASGAADVLEQATLCASLQEALADVSVAAALTSRRREIATPLLTPR
                     QACPSLLARAAAGEQVALVFGNETFGLSIEEVELCNVLVTIPGNPAYFSLNLAQAVQV
                     LAYELFSHTGQDLDALRPEVESATQQQVEGFYQHLEHTLTHIGFFERRPSERLMRRLR
                     TLFNRSQLQREEIDILRGMLKQIERHAPDRAQ"
     gene            3298..4080
                     /locus_tag="PKN55_03555"
     CDS             3298..4080
                     /locus_tag="PKN55_03555"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018748315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HNX70354.1"
                     /translation="MHPMLTIAVKAARRAASVIQRASNNLDILRPEQKRPNDFVSEVD
                     RASEQTIIDTLREAYPDHAILAEESGASRMGKSEYEWIIDPLDGTTNYLHGYPQYCVS
                     IALSVRGALTLGVIYDPCRNDLFTACRGSGAFLNDRRIRVGKRTMLADCLVATGHPHV
                     NTEKLDLDMAQLKAMIQRTVGVRREGSAALDLAYVACGRTDGYWEMNLNPWDIAAGAL
                     LVQEAGGIVTDLDGNENWLESGDILAANPKIMHQMLQVLNQA"
     gene            4246..5172
                     /locus_tag="PKN55_03560"
     CDS             4246..5172
                     /locus_tag="PKN55_03560"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNX70355.1"
                     /translation="MSASQGAPAVQRLTLTAADGYTLTARRFSPAGPARANLVMAGAT
                     AVPQKFYRRFAEFARQQGYDTLTLDYRGIGQSAPKSLRGFRMDYLDWGRLDLAAAVNL
                     MHDPSRPLFMVGHSYGGHALGVLPNHQLLDAFYVFGTGSGWHGWMPPLERLRIVLLWN
                     LVGPLVTRWKGYLPFKRMGMGENLPLDVYRQWKRWCSYPRYFFDDPARPELHEAFAQV
                     RMPIVAANSLDDAWASPRSRDAFMQGYRNADWQAHTIQPDAHTGPIGHMGYFRPSSQR
                     LWQEALDWFAQRKVMSAPPASAAIPPVVALLA"
     gene            complement(5183..5602)
                     /locus_tag="PKN55_03565"
     CDS             complement(5183..5602)
                     /locus_tag="PKN55_03565"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HNX70356.1"
                     /translation="MSQISVADFQALIDAELPLVGLFGIRTSDIGHGQARMRMHFNPG
                     LIRPGGTIAGPALMALADVTLYAVVLGMIGKVELAVTTSLNINFLRKPGQKDILAEGR
                     ILKLGQRLAVGEVLLYTEGEAEPVAHVTGTYSIPPRR"
     gene            5805..6017
                     /gene="rpmE"
                     /locus_tag="PKN55_03570"
     CDS             5805..6017
                     /gene="rpmE"
                     /locus_tag="PKN55_03570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012696882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HNX70357.1"
                     /translation="MKAGIHPDYKDLNVTCSCGHTFVTRSTMGKDAFHVEVCSQCHPF
                     YTGKQKVLDTAGRIDKFKQKFGKFAR"
     gene            6129..7778
                     /locus_tag="PKN55_03575"
     CDS             6129..7778
                     /locus_tag="PKN55_03575"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008954335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNX70358.1"
                     /translation="MLTYTPPSHPELPKPTEKPWLLLLLCFIWLWPGILGHGPWKPDE
                     PYALGVVQWMLHSGDWLTPRIAGEPYLDTPPLYYWVAALFARAFSPWLLPLHDAARLA
                     TPLFMSLTLLFVGMTARELIGRRHGRSAVLILIGCLGLIDTGHQLSADVAILCAEAAC
                     LLGLTLALRRGFLGALALALGSAALMLSASLVELVLLWMTAAMLPAFRGWRSRQYVKT
                     ITIALALGAPLVLMWPMSMAKHQPELFAQWWHSLALGPFYGFGRFALLHDFGYYLRLL
                     PWFAWPAWPLAAWAVWRGRERLSSTRYQLPLVLVLMNLLMLMLSPRQNPVYALPLLLP
                     LAVLAAVELDTLKRGAAAFFNWFGIFTLGLAGVLVWLGWTAMNFGWPAKLAAKAVKYS
                     PSYEAQIHVLPLLLALAMSLAWGWAVTRRRLMGRQAVTNWALGMTLLWSLAGTLWLPW
                     LDAQKGYHMVMHSLRASLPAQLDSCVASAPGNQLPRAMAYYYVNLPLLNSETVQGHSC
                     RYRLISTPEANYQPEGPWKMLWRGARPAEKREFYVLLKRQD"
     gene            complement(7857..10649)
                     /locus_tag="PKN55_03580"
     CDS             complement(7857..10649)
                     /locus_tag="PKN55_03580"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNX70359.1"
                     /translation="MWVRRKLSGLVLVVLTVGAAAPAALWALWDVQDTRLRLYQAFDQ
                     DIRQFQGKLASGLQAPLWDLNPELATPLLDAQFQDTRLVSLSVLTDTRQLFYLRDELR
                     RRDGHCATRYFPVLRQGRELGHTLAEFCDGVVQAQIARERDERLWLIGTQFGLTLLLI
                     VAMLHWRLVLPVRQLVAQARALAERRLLQPFLWRRQDELGQLGNALETTRQALAQLFS
                     RLEQQNQALLAELGQREAISQALAHSQRRYQALVDEAPEGIALLNPDSGQFIEGNPQA
                     ARLLGCRPDALARHTPFDAELVALPDAPALWQQRWRQALRAADDGALSQFRWRFQPLD
                     GQPQRHIDVHLGRLDTGGDRAIRATLLDVTAQVTAERQLHLLQRAVEQSHDGIAILDS
                     ELRLRSCNPGFYRLFSDEQTGQLLPGLSTDNPALYQALLTRLDSEGSWQDAMWLNTRA
                     GSRYCQVRISRIVSEEDPDNHICVVTDTTESHQQEDRIRYLAHHDPVTGLTNRHFLYG
                     RMAEAIHQLPPRPFALGFLDLDRFKTINDTLGHAAGDCLLQAVARRLQTLVRADDLVS
                     RIGGDEFVILASEMCCADEAAMLFSRVLAAFHDPFELEGKLFHISPSIGVCFFPADGD
                     TPDILMRNADTAMYAAKAAGRNTYQLFAQAMHAQNAERLDLEREMRNGVQAQQFVAYY
                     QPKVNADGELLCCEALVRWQHPALGVLSPARFLPLAEETGLIVAIGRQVLLAACHDCL
                     RWRQSGYPDMRVAVNVSSRQFSHDDLAATVQSCLDDSGLPPDALELEITESLLLDNLD
                     AAIRLLQALRALGVKISLDDFGTGYSSLSYLQKLPIDTLKIDRSFIQHIDSRSSDAAI
                     TRAIITLAHGLGVRVVAEGVETAGQCAVLVEMQCDLMQGFWFSRPVPAQTLAAMLGQR
                     LGGQCLLPEESAAEDS"
     gene            complement(10754..11908)
                     /locus_tag="PKN55_03585"
     CDS             complement(10754..11908)
                     /locus_tag="PKN55_03585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042925 - benzoate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0042919 - benzoate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoate/H(+) symporter BenE family transporter"
                     /protein_id="HNX70360.1"
                     /translation="MRRFFHPAHMSAGLIAVVVGYTSSAAIIFQAASAVGATPAQIGS
                     WLWALGIGMGVTSAGLAWWHKTPVLTAWSTPGAALLVTALAGVSMGEAVGAFLFASAL
                     AVLCGVTGWFDTLMQKVPKAIAAAMLAGVLLRFGLGAFTALPAQPWLVGGMLLGFLLL
                     RRHTPRFAVPLTLLIGLALAAAQGLIHLEKLTVSLAQPVWMAPEFSWSALIGVGLPLF
                     VVTMASQNMPGVAVLRANGYTLPVSPLVGWTGLAGVLLAPFGGFSFNLAAITAAICMS
                     PDADPDPAQRYRASIWAGLFYIATGMLGATVVGLFSALPQALILAIAGLALLGTIGNG
                     LASALEHPGERDAALMTFVVTASGMSLFGIGSAFWGLVLGLLTALLQRRG"
     gene            12008..12622
                     /locus_tag="PKN55_03590"
     CDS             12008..12622
                     /locus_tag="PKN55_03590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HNX70361.1"
                     /translation="MPPPTLPNTVTPPPLGDTLRALRKTRGWSLDQTAAATGVSKAML
                     GQIERGESSPTVATLWKLAGGLHISLSQLLEPPPTPPDTLLRDAASLRRQPASDAMLV
                     APLFPYDPQVGFEVFELTLLPGYTRRSEPHATGVIEYILVTAGAMDVWLDGDWRPLAQ
                     GQALRFAADREHGYRNQGETPAVFHNVIHYPCGHAQSLAEPTIR"
     gene            complement(12687..13175)
                     /locus_tag="PKN55_03595"
     CDS             complement(12687..13175)
                     /locus_tag="PKN55_03595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011134878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HNX70362.1"
                     /translation="MMAEHHADILWLRNGQDFLNQRYSRKHLLRFDGGIEVAGSSSPH
                     IVPLPMSEAAAIDPEEAFVASLSSCHMLWFLSIAAQQRFCVDRYADAAVGEMGANAEG
                     KTAMLRVTLRPEVVFSGAHQPSPAQIAQMHHTAHEACFIANSVKTEVCCQPVMHMGQA
                     LS"
BASE COUNT         2594 a         4315 c         3845 g         2506 t
ORIGIN      
        1 gggtgccttt ctcggaagac aagatcaatt ttttgcaggc gctggacaag ggctcagtgc
       61 cgatgaagca gttcatgatg cgccagaccc gcgaaaaaga tctggagttt ttcattgaag
      121 tttcgcaaag cgccaagcca cgcggcccag aagacatcag catgaaaacc ttggcgccgg
      181 cctttatcat cagcgagatg aaaaccgcct tccaaatcgg ttttatgatc tttattccct
      241 ttttgatcat tgacttggtg gtagcctcca ttctgatggc catgggtatg atgatggtgt
      301 cgccagtgac catcgccttg ccattcaagc tcatgctgtt tgtgctggtg gatggttgga
      361 ccctgctgct ggggtcgctg gtgcaaagtt tttacgtcag ttagcgagga tcgccatgac
      421 tccggaattc gtcatcaatc tggtgcaaaa cgccttgttc atgctgattg tggtgtccgc
      481 gccgattatg gcggtggcgc tgctggtggg tttgctggtc agcgtgctgc aggcagccac
      541 ccagatcaac gaaatgacgc tgaccttcat ccccaagctg ctggccatgt ttgtcgtgct
      601 ggtggtggcg ggtccgtgga tgatcaacac cctgatggat tacaccatcc gtttgttcac
      661 cagcattccc aacgctgtcg gctaaaaggc tgtcgccgct gtgtttcccg ctgccctgca
      721 tctgtctgat gcgcaaatct ctgccctgct cagccaatat ttgtggccgc tattccgcat
      781 tcttggtttt ttcatggccg agccgctgtt cagttcgcgc agcattccac gtcgcgccaa
      841 ggtgatcatg ggcatcatgc tgaccatcat gatttcaccc ttgctgccgc cgatgccggt
      901 gatcccggtg atttctggcg ccggattatt ggtgttgatg catcaggtgc tgattggcct
      961 ggccatgggc tatgtgatgc gcttgatgct gacagcagtg gaaatggccg gctttttcat
     1021 cagttcgcag atgggcctgg gttttgccat gttttttgac ccacagcact caggccaggt
     1081 gccgaccatc tcccgtttga tgtctctatt ggttttgcta ctgttgatgg cgatgaatgg
     1141 ccatcacctg atgctgtcca ccttgatcga cagcttcaag gtgtttccca tcactgccga
     1201 cggtttgccg ctgatcagct atcgcctgct ggccgagtgg ggggccgagg tgttttcact
     1261 gggtttgctg ttgtccatgc cagtgatggc ggcgctgctg atcgccaacg tggcgatggg
     1321 catcatgacc cgcgcggcgc cgcagttcaa cgtgttctcg tttggttttc cgctgaccat
     1381 caccattggc tttgtcgtac tgtacttcat gatgccgtac tttattgctg ccacccagat
     1441 gctttaccaa catggcacgg aactggtgct caccctgatc aaggccaagg ccgagccctg
     1501 agcctgcagc ggcaccgctc aggcctctgc cagcgtcagc atgtcggatt ccagccagct
     1561 gcccggcaca ataaaacccc gcccggcttc ttgccacacg ccttcgccgt tctgttgcag
     1621 gacggcgatt tgctgcggac tgtcataatc agccagctgt gcacgcaaac tcgccgcact
     1681 atcgcctccg gtctgcccgc cacaagcaga ccccagccag cgcaggcgcg gcaaatgcat
     1741 gtagcgcacc gaggcatctt gctcacccgg ccagggctct gccagtggcc gccaccagcc
     1801 cgctggcgcc aaaggattca agggcgccgg ggtgacaggg gcatcacggt gaaaaaatcg
     1861 cccgcgcagc agcgaaccca cctggcaatt ctgccagcca gcaggcaaca cggcctgtgc
     1921 cagcggatgc cggctcaggc gcaactgctg ctccagcttg gcaaacttgc gcaaccaggc
     1981 gtcgtgcaga tcaaccccca ccgcctgacg gccgaccacc agataatatt tgcatgccac
     2041 ttccacatgc cagggctcgc catccagccg tagcaagagg tcgaactcgc ccagcgtgcg
     2101 tccaccatcg cgcactggca gattggcgtg cagctcgctg caatgcggcg ccaactggca
     2161 ccaggcccac agcagcgcct caacatagcg ccctagccga cggctggccg ggcgctgtgc
     2221 caagatagcc agcaaagggg cgggggcgcg atccagttga tgcagcaagg ccagaccatg
     2281 cgcccccaat aaccgctcgc gcgggatttc gcagccggtg tgccaaggcg ccgcgccaag
     2341 cagcaattcg gccagatcgc gcaccacggc gtgctgaaaa tctgcggatg ccgtcattgt
     2401 gcgcggtcgg gcgcgtgccg ctcgatttgc ttgagcatgc cgcgcaggat gtcgatttcc
     2461 tcgcgttgca gctggctgcg gttaaacagc gtgcgcaagc ggcgcatcag ccgctcggat
     2521 gggcgacgct caaaaaagcc gatatgcgtc agcgtgtgct ccaggtgctg gtaaaaccct
     2581 tccacctgct gttgcgtggc ggattccact tccgggcgca gggcgtccag atcttgcccg
     2641 gtgtggctga acagctcata agccagcacc tgcaccgcct gggccagatt cagcgagaaa
     2701 tacgccggat tgccgggaat ggtcaccagc acattgcaca gctcgacttc ctcaatcgac
     2761 aagccaaagg tttcgttgcc aaacaccagc gccacctgct cgccggcggc ggcgcgcgcc
     2821 agcagcgatg ggcacgcttg gcgcggggtg agcagtggtg tggcgatttc gcgccggcgg
     2881 ctggtcagcg ccgccgccac gctgacatcg gccagggctt cttgcaggct ggcgcacagc
     2941 gtagcctgct ccagcacatc cgccgcgcca gaggccagcg tcaccgcctc tgtgctgggg
     3001 aattcccgtg gggccaccag cgtcagccgg gtcagcccca tggttttcat ggcgcgcgcg
     3061 gcggagccga tattgccagg atgcgacggc cgggccagca ccacgcgaat attttgcagc
     3121 gcagaggctg cttcgggttt catcattgga aaaatcacat taaaatgcag agcattacca
     3181 aacccgaaaa gtgtttcggg tttttttgtt cattaacaga cttccagcgc gcgccggcct
     3241 taaatgggtc ggccatcact cgccagcgtc ggcgcgcgca ctatcgaaag gccatccatg
     3301 catccgatgc tcaccatcgc ggtcaaagcc gcccgtcgcg ccgccagcgt catccagcgc
     3361 gcgtccaaca atctcgacat cctgcgtccc gagcaaaaac gcccgaacga ttttgtcagc
     3421 gaggttgacc gcgcgtccga gcaaacgatc atcgacaccc tgcgtgaggc ctacccggat
     3481 cacgccattc tagcagagga gtccggcgcc tcccggatgg ggaagtctga atatgagtgg
     3541 atcatcgatc cgctggatgg caccaccaac tatctgcacg gctatccgca gtactgcgtc
     3601 agcattgcgc tgagcgtgcg cggcgcgctc accctgggcg tgatttacga tccttgccgc
     3661 aatgacctgt tcaccgcctg ccgtggctct ggcgctttcc tgaacgaccg ccgcatccgc
     3721 gttggcaagc gcaccatgct ggccgattgc ctggtggcca ccggccatcc gcacgtcaac
     3781 accgaaaaac tcgacctgga catggcgcaa ctgaaagcca tgattcagcg caccgttggc
     3841 gtgcgccgcg aaggctcggc cgcgctggat ctggcctatg tggcctgcgg ccgcaccgat
     3901 ggctactggg aaatgaacct caacccgtgg gacatcgccg ctggcgcgct gctggtgcaa
     3961 gaagccggcg gcatcgtcac cgacctggac ggcaatgaaa actggctgga atccggcgac
     4021 atcctggccg ccaacccgaa aatcatgcac cagatgctgc aagtgctgaa tcaggcctga
     4081 tccttgctgg cgcactgaac gcaaaaccgc catccggata tttttcggat ggcggttttt
     4141 ttgtgcacgc gagcaaaagc atttagtagt tatacgatct tgtcatgtcc tggtgagcaa
     4201 tgacaccatc aatatttgtt tgctaacttg ttgatgaggt tcaccatgtc ggcttcccaa
     4261 ggcgcaccgg ctgtccagcg cctgaccctg acagccgccg acggctatac gctgacggcc
     4321 cgccgttttt cgccggccgg gccggctcgc gccaatctgg tgatggccgg cgccaccgcc
     4381 gtgccgcaaa aattctatcg ccgctttgcc gaatttgccc gtcaacaggg ctatgacacg
     4441 ctgacgctgg attatcgcgg cattggccaa tccgccccca aatcgctgcg tggctttcgc
     4501 atggactacc tcgactgggg ccggctggat ctcgctgctg ccgtaaacct catgcatgac
     4561 cccagtcgcc ccttgttcat ggtgggtcac tcctacggcg gccatgcgct gggcgtgctg
     4621 cccaatcacc agctgctgga tgcgttttat gtgtttggca ccggctcggg ctggcatggc
     4681 tggatgccgc cgctggagcg cttgcgcatt gtgctgctgt ggaatttggt tggcccgctg
     4741 gtcacccgct ggaaaggtta tttgccgttc aaacgcatgg gcatgggcga aaacctgccg
     4801 ctggatgttt accggcagtg gaagcgctgg tgcagctatc cacgctactt ttttgacgac
     4861 ccggcacggc cggaactgca cgaggcgttt gcccaggtgc gcatgccgat tgtcgccgcc
     4921 aactcgctgg acgacgcctg ggcctcgccg cgctcgcgcg atgctttcat gcaaggctac
     4981 cgcaacgctg actggcaggc gcacaccatc cagccagacg cccataccgg gccaattggg
     5041 catatgggtt acttccgccc cagctcacag cggttgtggc aagaggcact ggactggttt
     5101 gcccagcgca aggtcatgtc agcaccaccc gccagcgcag ccatcccgcc cgtggtggcg
     5161 ctgctggcct gagcggacag ggtcagcgcc ggggtggaat ggaataggtg ccggtgacat
     5221 gcgccaccgg ctcggcttca ccctcggtgt acagcagcac ttcgcccacc gccagccgct
     5281 ggcccaactt gagaatgcgg ccttcggcca ggatgtcctt ctggccgggt ttgcgcaaaa
     5341 aattgatgtt caggctggtg gttactgcca gctccacctt gccgatcatg cccagcacca
     5401 cggcatacaa ggtgacatcc gccagcgcca tcagcgccgg gccggcaatg gtgccgcccg
     5461 ggcggatcag accagggttg aagtgcatgc gcatgcgcgc ctggccatgg ccgatgtcgc
     5521 tggtgcggat gccaaacaag cccaccaagg gcaactcggc gtcaattaaa gcctgaaaat
     5581 cggcaacaga aatctggctc acagcaagct ccagcagggc ggggaaaaca gcagcatggt
     5641 cagtgggcgc ctgttgcgtc aatcttgcga cgcaccatca cgagcctgct tgcccaaaca
     5701 ggcatggcgc gcggcttgtc aagtatttgc gtatggcgca taatgccggt ttcagcaaag
     5761 tagcgtaggc aaaccccctg cgcccgtgac ccaaaggaaa aaccatgaaa gctggcatcc
     5821 atccggatta caaagacttg aacgtgacct gctcctgcgg ccacaccttc gtcacccgtt
     5881 ccaccatggg caaagacgcc ttccacgtgg aagtgtgctc gcagtgccac ccgttctaca
     5941 ccggcaagca aaaggtgctg gacaccgctg gccgcatcga caagttcaag caaaagttcg
     6001 gcaagttcgc tcgttaatcg acgaaccccc aacaaaaaaa ggcagcctcg gctgcctttt
     6061 tttgttggct gcaatcccgc acaatatcgc ctttgccacg tcggcaaccc tgccaagcct
     6121 atgcgtctat gctgacctat accccgccct cgcaccccga actgcccaag ccaacagaaa
     6181 agccctggct gttgctgcta ttgtgcttta tctggctgtg gccgggcatt ttgggccatg
     6241 gcccgtggaa acctgatgag ccttatgccc ttggtgtcgt gcagtggatg ctgcacagcg
     6301 gcgactggct gacaccacgc attgccgggg aaccgtatct ggacacccca ccgttgtatt
     6361 actgggtggc tgcgctgttt gcccgggcat tttctccatg gctattgccg ctgcatgacg
     6421 cggcgcggct ggccactccc ttgttcatgt cgctgactct gctgtttgtc ggcatgaccg
     6481 cacgcgagct gatcggccgc cgccatggtc gcagtgccgt gttgatcctg attggctgcc
     6541 tgggtttgat cgataccggt caccagctca gcgccgacgt agccatcctg tgcgccgaag
     6601 cggcctgcct gctggggctg acacttgcct tgcgccgtgg ttttctcggg gcgctggcgc
     6661 tggcgctggg cagtgccgcc ctgatgctgt cggccagtct ggtggaactg gtgctgctgt
     6721 ggatgacggc cgccatgttg cccgcgtttc gtggctggcg cagccgccag tatgtcaaaa
     6781 ccatcaccat tgccttggcc ctgggcgcgc ctttggtgct gatgtggccg atgtcgatgg
     6841 ccaaacacca gccggaactg tttgcccagt ggtggcattc gctggcgctg gggccgtttt
     6901 atggctttgg ccgctttgcc ctgctgcacg attttggcta ctacctgcgc ctgctaccat
     6961 ggtttgcctg gcccgcctgg ccgctggccg cctgggcggt ctggcgtggt cgggagcggc
     7021 tgtcgtccac ccgctatcag ctgccgctgg tgctggtgct gatgaacctg ctgatgctga
     7081 tgctgtcgcc ccgtcagaac ccggtctatg cactgccgct attgctgccg ctggccgtac
     7141 tggctgccgt ggaactggac accctcaaac gtggcgccgc tgcgttcttt aactggttcg
     7201 gtattttcac tctggggctg gccggtgtgc tggtctggct gggctggacg gcgatgaatt
     7261 ttggctggcc tgccaaactg gccgcaaaag cggtcaaata cagccccagt tatgaagccc
     7321 agattcatgt cctgcccttg ctgctggcac tggccatgtc gctggcctgg ggctgggcag
     7381 tcacgcgccg ccggctgatg ggacgccagg ccgtgaccaa ctgggcgctg ggcatgacct
     7441 tgctgtggag tctggccggc accctgtggt tgccatggct ggatgcccag aaaggctacc
     7501 atatggtcat gcactcactg cgcgccagcc tgcccgcaca actggacagc tgcgtggcca
     7561 gtgcgccggg caaccagctg ccgcgcgcca tggcgtatta ctatgtcaat ctgccgctgc
     7621 tcaatagcga aaccgtgcag ggccacagtt gtcggtaccg tttgatcagc acaccagaag
     7681 ccaattatca gccggaaggc ccgtggaaaa tgctgtggcg tggcgcccgg ccggcggaga
     7741 aacgcgagtt ttatgtgctg ctcaaacgcc aggactaaaa accgccaaca acactcagcg
     7801 caacgctcct gagacagtgc ggggatcgtg cagcaacgcc agagggttag cggcacttaa
     7861 ctgtcttcgg cggcagattc ttccggaagc aaacactgac cacccaggcg ctggcccagc
     7921 atggctgcca gcgtctgggc cggcaccggg cggctgaacc agaagccctg catcagatca
     7981 cattgcattt caaccagcac cgcacactgg ccggcggttt ccaccccttc ggccaccacg
     8041 cgcaccccca gcccgtgcgc cagcgtgata atggcgcggg taattgccgc atcgctgctg
     8101 cggctgtcga tgtgctgaat gaaactgcgg tcgatcttca gcgtatcgat gggcagtttt
     8161 tgcaaatagc tcaggctgga atacccggtg ccaaaatcat ccagcgaaat cttcaccccc
     8221 agtgcacgca aggcttgcag caggcggatg gcggcatcca gattgtccag cagcaggctt
     8281 tcggtgattt ccagctccag cgcatcgggc ggcaggccgc tgtcatccag acagctctgc
     8341 acggtcgccg ccagatcatc atggctgaac tggcggctgg acacattcac cgccacccgc
     8401 atgtccggat agcctgattg ccgccagcgc aggcaatcgt gacaagccgc cagcagtacc
     8461 tgacggccaa tggcgacaat caggccggtt tcttccgcca gtggcaaaaa gcgggcggga
     8521 gacaacaccc cgagtgccgg atgctgccag cgcaccagcg cctcacaaca cagcagctcg
     8581 ccgtcggcgt tgaccttggg ctggtaatag gccacaaact gctgggcctg cacaccgttg
     8641 cgcatctcgc gctccagatc cagccgttcg gcattctgtg cgtgcatggc ttgggcaaac
     8701 agctgatacg tattgcgccc ggccgccttg gccgcataca tcgccgtatc ggcattgcgc
     8761 atcaggatat ctggcgtatc gccatcggcc gggaaaaagc acaccccaat gctggggctg
     8821 atatggaaca acttgccttc cagctcgaat ggatcatgaa acgccgccag cacccgcgaa
     8881 aacaacatgg ccgcctcgtc agcacagcac atctcgctgg ccaggatgac aaattcgtcg
     8941 cccccgatgc gtgacaccag atcgtccgca cgcaccaggg tttgcaagcg ccgggccacc
     9001 gcctgcaaca ggcaatcgcc agcggcgtgc cccagcgtgt cgttgatggt tttgaagcga
     9061 tccaggtcga gaaagcccag ggcaaacggg cgcggtggca attgatgaat cgcctcggcc
     9121 atccgcccat acaaaaaatg ccggttggtc agcccggtga ccggatcgtg gtgcgccaga
     9181 tagcggatgc gatcttcctg ctgatggctt tccgtggtgt ccgtcaccac acagatatga
     9241 ttgtccgggt cttcctcgct gacgatacga ctgatgcgca cctggcaata gcggctgccg
     9301 gcgcgagtgt tcagccacat ggcatcctgc cagctgcctt cgctgtccag tcgcgtcagc
     9361 agcgcctgat aaagcgccgg gttatcggtg ctcaggcctg gcagcaactg gccggtctgc
     9421 tcgtcgctga acaagcggta aaagcccggg ttgcaactgc gcagacgcag ctcgctgtcc
     9481 agaatggcga tgccatcgtg gctttgctcg acggcgcgtt gcaataaatg caactggcgc
     9541 tcggccgtca cctgggccgt cacgtccagc aaagtggctc ggatggcccg atcgccgccg
     9601 gtatccagcc ggcccagatg gacatcgata tgtcgttgtg gctggccatc cagcggctga
     9661 aaacgccagc gaaactggct aagcgccccg tcatccgctg cacgcaaagc ctgtcgccag
     9721 cgctgctgcc acagggctgg agcatctggc aaagcgacca gttcggcatc aaaaggggta
     9781 tggcgggcca gggcatccgg ccggcagccc agcaaacgcg ccgcctgtgg attgccctca
     9841 ataaattggc cgctgtccgg attcagcagg gcaatacctt ccggcgcctc atccaccagc
     9901 gcctgataac gacgctggct gtgcgccagc gcctggctga tggcctcacg ctggcccagc
     9961 tcagccagca gggcctggtt ttgctgctcc agtcgggaaa acaactgcgc cagtgcctgg
    10021 cgagtggttt ccagtgcatt acccaattgc cccagctcat cctgacgccg ccacaaaaat
    10081 ggctgcaata gccggcgctc ggccagggca cgcgcctgtg ccaccaactg acgcaccggc
    10141 aataccagcc gccagtgcag catggccaca atcagcaaca gggtcagccc gaactgcgtc
    10201 ccgatcagcc acaaccgctc gtcgcgctca cgggcaatct gcgcctgcac cacgccatca
    10261 caaaactccg ccagggtatg ccccagctcg cgaccttgcc gcagcaccgg aaaataccgg
    10321 gtcgcacagt ggccatcccg gcggcgcaac tcgtcacgga ggtaaaacaa ctgacgggtg
    10381 tcggtcagta cactcaggct gaccagacgg gtgtcctgaa actgggcatc cagcaagggc
    10441 gtggccagct cggggttaag gtcccacaat ggcgcttgca agccagacgc cagcttgccc
    10501 tgaaactgac ggatatcctg atcaaatgcc tgatacaggc gcaggcgcgt atcctgcaca
    10561 tcccacaagg cccacaaagc cgccggggcg gccgccccca cggtcagcac caccagcacc
    10621 agcccagata acttgcgacg aacccacatg cagccttcct tcccggtggc gtggacacat
    10681 catggcaatt tccctaccgc cggtctgact atagagtaat cctgcgtcag ccgcatgacc
    10741 acatgggcgc cgctcagcca cgtcgctgca acagcgcggt cagcaggccc agcaccagcc
    10801 cccagaatgc gctgccaatg ccaaacagcg acatccccga agccgtcacc acaaaggtca
    10861 tcaaggccgc gtcgcgctcg ccggggtgct ccagcgcgct ggccagaccg ttgccaatcg
    10921 tgcccaatag cgccaggccg gcaatcgcca gaatcagcgc ttgtggcagg gcgctgaaca
    10981 agcccaccac cgttgcgcca agcatgccgg tggcaatata aaacaagccc gcccagatcg
    11041 acgcccgata gcgctgggcc gggtcggggt cggcgtccgg gctcatgcag atggcggcgg
    11101 tgatggcggc cagattaaac gaaaacccgc caaacggcgc cagcagcacg ccggccagcc
    11161 cagtccagcc caccagcggc gaaaccggca acgtataacc attggcgcgc aacaccgcca
    11221 cgccaggcat attctgcgag gccatggtca ccacaaacag cggcaggccc acgccaatca
    11281 gcgctgacca cgaaaattcc ggcgccatcc ataccggctg cgccaggctg acggtgagtt
    11341 tttccagatg aatcaaaccc tgcgccgccg ccagtgccag gccaatcagc agggtcagcg
    11401 gcacggcaaa gcgcggcgtg tggcggcgca gcaacaaaaa gcccagcaac atgccgccca
    11461 ccagccacgg ttgcgccggc aaggcagtaa acgcgcccag accaaagcgc agcagcaccc
    11521 cagccagcat ggcggcggca atcgccttgg gcactttctg catcaacgta tcaaaccaac
    11581 cggtgacccc gcacagcacc gccaacgccg aagcaaacaa aaacgcgccc accgcctcgc
    11641 ccatcgacac cccggccagc gccgtgacca acagcgctgc gcccggcgta gaccacgccg
    11701 tcagcaccgg ggttttatgc caccaggcca ggccagcact ggtcaccccc atgccgatgc
    11761 ccaatgccca tagccacgaa ccgatttgcg ccggcgtggc gcccaccgcg ctggctgcct
    11821 gaaaaataat tgccgccgaa ctggtgtagc ccaccaccac ggcaatcagc ccggctgaca
    11881 tatgggccgg atgaaaaaaa cgacgcatgc aaaccccttt atccctaacc cggcatgaca
    11941 gaccgacaga tggattgtta tgatgagagg atgctaaccg atgtgcgcca taacgcacaa
    12001 gcttgccatg ccaccaccca cgttgcccaa taccgtcacc ccgcccccac tgggcgacac
    12061 cctgcgcgcg ctacgcaaaa cccgcggctg gagcctggac caaaccgccg ccgccactgg
    12121 cgtcagcaaa gccatgctgg gccagatcga acgtggcgaa tccagcccca ccgtggccac
    12181 actgtggaag ctggccggcg gcctgcatat ctcgctctcg caactgctgg aaccgccgcc
    12241 caccccgccc gacaccctgc tgcgcgacgc cgccagcctg cgccgccaac cggccagcga
    12301 cgccatgctg gtggcgccgt tgtttccgta tgatccgcaa gtgggttttg aagtattcga
    12361 gctgacgctt ttgcccggct acactcgccg ttctgaaccc cacgcgacgg gggtgattga
    12421 atatattttg gtgacagcgg gcgccatgga tgtctggctg gacggcgact ggcgcccgct
    12481 ggcccaaggc caggccctgc gctttgccgc cgaccgtgaa cacggctacc gcaatcaagg
    12541 agagacgccg gcagtgtttc acaatgtgat tcactacccc tgtggccacg cgcaaagcct
    12601 ggcggaaccc accatccggt gactcacccc acctgatcac gggacaaggc cggacacgcc
    12661 cgcccggaag attttgtgag cagctcttaa gacaaagcct gacccatgtg catgaccggc
    12721 tggcagcaca cttcggtttt gaccgaattg gcaataaaac acgcctcatg cgcggtgtga
    12781 tgcatttgcg caatctgggc cgggcttggc tgatgcgcgc cggaaaacac cacctccggc
    12841 cgcagcgtca ccctcagcat cgccgtcttg ccctctgcat tcgcccccat ctccccgacg
    12901 gccgcatcgg cgtagcggtc aacacaaaac ctctgctggg cggcaatcga caaaaaccac
    12961 agcatatggc agctggacag cgaggcgaca aaggcttctt ccgggtcgat ggccgccgcc
    13021 tcagacatcg gcaagggcac aatatgcggc gacgacgacc ccgccacctc gatgccgccg
    13081 tcaaaccgca gcaggtgttt tctgctgtag cgctggttta aaaagtcctg cccgttgcga
    13141 agccatagga tgtcggcgtg gtgttcagcc atcatggtgt gctcgaaaaa aattgcatgg
    13201 tcaaattagc gcggcgctca ctggcgagaa ggcgtcagct gatggacgcc agcgcctcac
//
LOCUS       DAOPYX010000042        12842 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_5396_length_12842_cov_10.752327, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000042 DAOPYX010000000
VERSION     DAOPYX010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 12842)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12842)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12842
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5396_length_12842_cov_10.752327"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..764)
                     /gene="motD"
                     /locus_tag="PKN55_03600"
     CDS             complement(<1..764)
                     /gene="motD"
                     /locus_tag="PKN55_03600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008954366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotD"
                     /protein_id="HNX70363.1"
                     /translation="MRRRPPEDPDHHERWMVSYADFVTLLFAFFVVMYAISSINEGKY
                     KQMTQSMIAAFNQGSISPKVIGQSGSANTHVAVEDANPMTSKVAASLKQQQDMRMRQL
                     AAQVRQLLDPLMQRGQVRVTDSPLGIAVEINDSALFESGQATPSTASVSWLGRLGGVL
                     KGHPNRIRVEGYTDDRPIRTSVYPSNWELSAARAGSVVRLFVENGVASPRLVAIGRAE
                     NRPVASNDSADGRARNRRVTISILPESAGDNDLVPVA"
     gene            complement(786..1724)
                     /locus_tag="PKN55_03605"
     CDS             complement(786..1724)
                     /locus_tag="PKN55_03605"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018748896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HNX70364.1"
                     /translation="MSEPQWERQVLEKLATAALTEQRRTRQWKLFFRLVWVLLAILLL
                     YGLLRNGDSEGALASTGDHVALLTLSGAISAENHTADRMIAGLQKAYKSSGTRAVVIR
                     ANSPGGSPVLSGMVNDEIRRLKGEHPDIPVYVVVEEVCASGCYYIAAAADKIFVDKAS
                     IVGSIGVLSDGFGFTGALEKLGVERRLMTAGSHKALADPFSPRKAEDEAIRQALLDDI
                     HRQFIQVVKTGRGARLKDDPTIFTGLYWLGEKSIPLGLADGYGTVDSVARELVKTDNI
                     VDVTPSDDLVDRFTKRFGVMLSAALLSQGDSGPQLK"
     gene            complement(1773..2117)
                     /locus_tag="PKN55_03610"
     CDS             complement(1773..2117)
                     /locus_tag="PKN55_03610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008954368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HNX70365.1"
                     /translation="MADAAPGQLIGAASALQDSRPGLRFTLPDGRPGIAVRFRGQVYA
                     YVNTCAHVPVELDLVAGDVFDISGQYLVCAMHGAYYDPQTGRCLGGPCPGRRLTPLTV
                     IERDGQVWLASA"
     gene            complement(2090..2767)
                     /locus_tag="PKN55_03615"
     CDS             complement(2090..2767)
                     /locus_tag="PKN55_03615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012696549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HNX70366.1"
                     /translation="MTLPADAHAFDLVVFDWDGTLMDSTAHITRSIQRACADLGLPVP
                     ERARASHVIGLGLVDAMRHVCPGLPAERYQDMVNAYRTHYLAGDQAIELFDGVAEALA
                     RWRARGVLLAVATGKSRIGLDRALAATGLGECFAITRTVDECRSKPHPQMLDDITEFL
                     GADKRRTLMVGDTTHDLQMAQNAGTWGAGVSYGAHPVEALRDCAPLACFDSFAEFDTW
                     LTPRLAN"
     gene            complement(2764..3702)
                     /locus_tag="PKN55_03620"
     CDS             complement(2764..3702)
                     /locus_tag="PKN55_03620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019389524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HNX70367.1"
                     /translation="MTDLRKDSVTFVTVDDAGHDQRVDNFLLRHLKGVPKSHVYRILR
                     SGEVRVNKGRVDASHRLQHGDVLRIPPVRVAQSATPAGRAAPAQEFAIVYEDDALLVI
                     DKPAGVAVHGGSGVSFGVIEQLRAARPEARFLELVHRLDRETSGLLMLAKKRSALVKL
                     HEMIRDNHMDKRYQALGVGDWPEARKQVKLPLLKFNLPDGERRVRVADDGQHAHTNFR
                     VLKRASGFVLLEAQLKTGRTHQIRVHMAASGAPIAGDEKYGDYAFNRELHKRGLKRMF
                     LHAWRLRLNHPLSGAPLELLAPLPRELQSFLDTLSA"
     gene            4415..7810
                     /gene="rne"
                     /locus_tag="PKN55_03625"
     CDS             4415..7810
                     /gene="rne"
                     /locus_tag="PKN55_03625"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018748900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008995 - ribonuclease E activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HNX70368.1"
                     /translation="MKRMLFNATQAEELRVAIVDGQKLIDLDIETVGKEQKKGNIYKG
                     VITRIEPSLEACFVDYGTERHGFLPFKEVSRAYFRQFDGGGRPRIQDVLREGQEIIVQ
                     VEKDERGNKGAALTTYVSLAGRYLVLMPNNPRGGGVSRRIEGEERNELRELLAQLETP
                     AGMSLIARTAGIGRSLEELQWDLNYLLQLWTAIESAAGAQNAPFLIFQEGSLVIRAIR
                     DYFQPDIGEILIDTEEIHAQARQFMSHVMPGNVNRVKLYKDDVPLFSRFQIEHQIETA
                     FSRAVTLPSGGAIVIDHTEALVSVDVNSARATKGSDIETTALNTNLEAADEIARQLRL
                     RDLGGLIVIDFIDMENPKNQREVENRLREALKTDRARVQMGKLSRFGLLELSRQRLQP
                     SLGESSHMPCPRCHGTGFIRGIESSALHILRIIQEEAMKDNTCAVHAQVPVDVATFLL
                     NEKRADLFTIEARLKVSVVLIPNIHLETPHYKIVRLRADDLPEGEEPSYKMVETPTEE
                     NGYAGSQPEKAKPERQQAAVQGITPAQPAPVSARDQEPAPAPAAAAPAAAAPAPSPVA
                     AAPAQPGVMARLVGWVKSLFTTEVPAPAPAPEPVKPAAASPRREGNGQRRDRDRRPRP
                     PRDDSAPRRRDDNRSERAERAERDADAPRPEREPRAPRAERQERAERPERAEREPREQ
                     REPREPRERSERPERGSRPERQALRHEMREREEREAREQQQGERPNAAPADADAPRAE
                     RAERPERRRRQPEAAPAAEAVEAELKAAEAVAQTAAAQDEQADVAPAEGEAREPRSRR
                     RRGRRGGERRRDEAQADGNLEAAAPQIDPETQHDAVPSAAAPVAAAVAVAAAAVAVEV
                     AAETPLASAVQEAVPVAAPEAEQAAAPVAVIPVAVPTPLEPVAVAASAPVAVETVPMP
                     EPQAQVVVDEPVLAPAVSVAPVELPPVAVAAVVEAVVVADAVAVEPEVALAEHAVASI
                     EPVAVQPAVEPVAEVAVPAMPEPLAAPVSIAEAIALAEPVSEPVVAEPSAAEPVSPVA
                     EPAVASVALGGLELVSTRVAEPLAVMDEAQAPRRRRRDVAESLAAGTEQAALQQVETL
                     NLPAAVPQAAASLATAQPVRRRDRLAAQSGVEAVEMVQVQTRD"
     gene            7915..7990
                     /locus_tag="PKN55_03630"
     tRNA            7915..7990
                     /locus_tag="PKN55_03630"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7948..7950,aa:Asn,seq:gtt)
     gene            8117..8521
                     /locus_tag="PKN55_03635"
     CDS             8117..8521
                     /locus_tag="PKN55_03635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX70369.1"
                     /translation="MRGLWRAVQACGVVALLAVAGVACEQRNPVFIGIWMCGDMELQF
                     SGDGRFRWRGVDGDELNGNFHLVRDGSAYRIDYQPVRWAVQEKRYIPTRYPVDGFYLS
                     ATPSGEGGDMLACQRKRVRGAARVLSWLDEGP"
     gene            complement(8606..8681)
                     /locus_tag="PKN55_03640"
     tRNA            complement(8606..8681)
                     /locus_tag="PKN55_03640"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8646..8648),aa:His,seq:gtg)
     gene            complement(8744..8820)
                     /locus_tag="PKN55_03645"
     tRNA            complement(8744..8820)
                     /locus_tag="PKN55_03645"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8784..8786),aa:Arg,seq:tct)
     gene            complement(8839..8916)
                     /locus_tag="PKN55_03650"
     tRNA            complement(8839..8916)
                     /locus_tag="PKN55_03650"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8879..8881),aa:Pro,seq:tgg)
     gene            9125..9976
                     /gene="folD"
                     /locus_tag="PKN55_03655"
     CDS             9125..9976
                     /gene="folD"
                     /locus_tag="PKN55_03655"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012696501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HNX70370.1"
                     /translation="MAAQLIDGKTISEALLNRVKAGVDARLAEGRRAPALAVVLVGNN
                     PASEIYVRNKKRSCEKVGIRSVAFDLPASTTQAELLAIVDQVNADPEIDGILVQLPLP
                     AHIDADAVIERIDPKKDVDGFHPYNIGRLALKMPLLRPCTPRGVMTMLEMSGIDPKGK
                     HAVIIGASNIVGRPMALELLLARATVTVCHSATADLAAEVRRADIVVAAVGKPGFVPG
                     DWIKEGAVVVDVGINRLPDGKVVGDVDFAAASERASWITPVPGGVGLMTVATLLQNTL
                     DSANLHA"
     gene            10015..10875
                     /gene="purU"
                     /locus_tag="PKN55_03660"
     CDS             10015..10875
                     /gene="purU"
                     /locus_tag="PKN55_03660"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012696500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HNX70371.1"
                     /translation="MSERNSATLLISCPDRKGLVATIANFLMAYNANILHADQHQDSS
                     ENLFLMRVEWDLSGFTLPMENFAAAFQPIADENQMTWRVTLSSKKPRMALFVSKYEHC
                     LVDLMHRWRIGELNCDIALVISNHEDCRRLVEFNGIPFHVVPVTRDNKAEMEARQWAL
                     LEEAQVDFIVLARYMQVLSPSFVERYPSRVINIHHSFLPAFDGAKPYHRAFARGVKLI
                     GATSHYVTEVLDDGPIIEQEVTRISHRDDVEDLVQKGRDLEKVVLSRAVRWHIENRIL
                     AYANKTVIFD"
     gene            10928..11470
                     /locus_tag="PKN55_03665"
     CDS             10928..11470
                     /locus_tag="PKN55_03665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX70372.1"
                     /translation="MRLIDDLIELACLRARPLAEYRHPAWQLLCALLILSVINSASAE
                     NFTGPLPARLGFFAVFALLESVLVTAVLTLWLRLSGWRERDSLWPLVVLSSGVQALEP
                     LTSWLGEAAGQTLSGLLAVYSVLVLVLGVARATGMPAWRVAAGVVMFLPLGAMLLAVM
                     LGAAADMGWVTLPVAPADAN"
     gene            complement(11883..12452)
                     /locus_tag="PKN55_03670"
     CDS             complement(11883..12452)
                     /locus_tag="PKN55_03670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX70373.1"
                     /translation="MQINTNASTTLPLNSKSSGKSISTPEGQECTSVTWTAFTTSAEK
                     SRELELRYAEIMTGLKGEEALSKHKKDMEKTMALMGPETREHRPSAAELEKQLQMQAD
                     SIFRGPAGNILAVVYKDGSAYSHANIDLSALAASAKGLSEHEYRAAMRNGVAAALGSR
                     AVASYYDYNKPAPTMREIHEEEHAFNRRA"
     gene            complement(12513..>12842)
                     /locus_tag="PKN55_03675"
     CDS             complement(12513..>12842)
                     /locus_tag="PKN55_03675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX70374.1"
                     /translation="LGGFGDDLYVQYIGYGNGQDTINDGVTASYTPGYGGGIDKLYIP
                     NVTLDQLIPLRQANDLWISSMADASDGQITEGVVISNFFLGGIYTIEYLETQDYILSL
                     SDYFGVV"
BASE COUNT         2362 a         3978 c         4062 g         2440 t
ORIGIN      
        1 gccaccggca ccagatcatt atcgccagcg ctttccggca ggatgctgat ggtcacccgg
       61 cggttgcggg cgcggccgtc ggcgctgtcg ttgctggcca ccgggcggtt ttcggcgcgg
      121 ccgatggcca ccaggcgcgg cgaggcgacg ccgttttcga caaacaggcg caccacgctg
      181 ccggcgcggg cggcggataa ttcccagttg gaggggtaga cgctggtgcg aatcggccgg
      241 tcgtctgtgt agccttccac ccggatgcgg ttgggatggc ctttcagcac gccgcccagc
      301 cggcccagcc agcttaccga ggcggtggag ggggtggcct ggccggattc aaacagcgcg
      361 ctgtcgttga tttccacggc aatgcccagc gggctgtcgg tcacccgcac ctggccgcgc
      421 tgcatcagcg ggtccagcag ctggcgcacc tgcgccgcca gctggcgcat gcgcatgtct
      481 tgctgctgtt tcagcgaggc ggccaccttg ctggtcatcg ggttggcgtc ttccaccgcc
      541 acatgggtgt tggcgctgcc ggactggcca atcaccttgg gcgagatcga gccctgattg
      601 aacgccgcaa tcatggattg ggtcatctgc ttgtatttgc cctcgttgat cgacgagatg
      661 gcgtacatca ccacaaaaaa agcaaacagc aaggtgacaa agtcggcgta ggacaccatc
      721 cagcgttcgt ggtggtcggg gtcttccggc ggacggcggc gcatgggcgg ggctctcctg
      781 cgggcttatt tcagttgcgg gccgctgtcg ccctggctca acagcgcagc ggacagcatc
      841 acgccaaagc gtttggtgaa gcggtccacc agatcgtcgc tgggggttac atcgacaata
      901 ttgtcggttt tcaccagctc gcgcgctacg ctgtccaccg tgccatagcc gtcggccagg
      961 cccagcggaa tgcttttttc acccagccag tacaggccgg tgaaaatagt cgggtcgtcc
     1021 ttcagtcggg cgccgcgccc agttttcact acctgaatga actgacggtg aatatcgtcc
     1081 agcagcgcct ggcggatggc ttcatcttct gccttgcgcg gcgagaacgg gtcggccagc
     1141 gccttgtggc tgccggcggt catcagccgg cgctccacgc cgagtttttc cagcgcgccg
     1201 gtaaagccaa agccatcgga cagcacgcca atcgagccga caatgctggc cttatcgaca
     1261 aaaatcttgt cggcggcggc ggcgatgtaa taacaacccg aggcgcagac ttcttcaacc
     1321 accacataca ccggaatatc cgggtgctcg cccttcagcc ggcgaatttc gtcattgacc
     1381 atgccggaca gcaccgggct gcccccgggg ctgttggcgc gaatcaccac ggcgcgcgtg
     1441 ccgctgctct tgtaggcttt ttgcaggccg gcaatcatgc gatccgccgt gtggttttct
     1501 gcgctgatgg cgccggatag cgtcagcagc gccacatggt cgccggtgct ggccagcgcg
     1561 ccctcactgt cgccgttgcg cagcaggcca tacagcagca agatggccag cagcacccac
     1621 accaggcgga aaaacagctt ccactggcgg gtgcggcgtt gctcggtcag ggcggcggtg
     1681 gccagttttt ccagcacctg gcgttcccac tgcggttcac tcatgcaaga tttcctgtgg
     1741 ctggcgcacg cgcggggcgc ggcgcatcgg gttcaggcgg acgcgagcca tacttggccg
     1801 tcgcgctcaa tcacggtcag cggcgtcagc cgccggcctg ggcaggggcc gcccaggcag
     1861 cggccggttt gcggatcata gtacgcgcca tgcatggcgc acaccagata ctggccggaa
     1921 atgtcaaaca catcgcctgc caccaaatcc agttcgaccg gcacatgcgc gcaggtgttg
     1981 acataggcgt acacctggcc gcgaaagcgc acggcaatgc cggggcggcc gtcgggcagg
     2041 gtgaagcgca ggccagggcg gctgtcttgc agcgcgctgg cggcgccgat cagttggcca
     2101 ggcgcggcgt cagccatgtg tcgaactcgg caaacgaatc aaaacacgcc agcggcgcgc
     2161 agtcgcgcag ggcttccacc gggtgcgcgc cgtagctgac gccggcgccc caggtgccgg
     2221 cgttttgcgc catttgcaaa tcgtgggtgg tgtcgcccac catcagcgtg cggcgcttgt
     2281 cggcgcccaa aaactcggtg atgtcgtcca gcatctgcgg atgcggcttg gagcggcatt
     2341 cgtccaccgt gcgggtgatg gcaaagcact cgcccaggcc cgtggcggcc agcgcgcggt
     2401 ccaggccgat gcggctcttg ccggtggcca ccgccagcag cacgccgcgc gcgcgccagc
     2461 gcgccagcgc ctcggccacg ccatcgaaca gctcgatggc ctggtcgccg gccaggtagt
     2521 gggtgcggta ggcgttgacc atgtcctggt agcgctcggc cggcaggccg gggcaaacat
     2581 ggcgcatggc gtccaccagc cccaggccga tcacatggct ggcgcgggcg cgctcgggca
     2641 ccggcaggcc caggtcggcg caggcgcgct ggatcgagcg ggtgatgtgg gcggtggagt
     2701 ccatcagggt gccatcccag tcaaacacca ccagatcaaa agcgtgggca tcagcaggca
     2761 gggtcatgcg ctcagggtat ccagaaaaga ttgcaattcg cgcggcagcg gggcgagaag
     2821 ctccagcggc gcgccgctca gcgggtgatt caggcgcagc cgccaggcat gcagaaacat
     2881 gcgcttgagc ccgcgcttgt gcagctcacg attgaaagca tagtcgccat atttttcatc
     2941 gccagcaatc ggcgcgccgc tggcggccat gtgtacgcga atctggtggg tgcggccggt
     3001 tttcagctgg gcttccagca gcacaaagcc gctggcgcgc ttgagcacgc gaaaattggt
     3061 gtgcgcatgc tggccatcgt cggccacccg cacccggcgc tcgccgtcgg gcaggttgaa
     3121 cttgagcaag ggcagcttga cctgcttgcg cgcctcgggc cagtcgccca cgcccagcgc
     3181 ctgatagcgc ttgtccatgt ggttgtcgcg gatcatttcg tgcagcttga ccagcgccga
     3241 gcgttttttc gccagcatca acaggccgga ggtttcccgg tccagccggt gcaccagctc
     3301 cagaaagcgc gcctcgggcc gcgcggcgcg caattgctcg atcacgccaa acgacacgcc
     3361 cgagccgcca tgcaccgcca ccccagccgg tttgtcgatc accagcaggg cgtcgtcttc
     3421 gtagacgatg gcgaattcct gcgcgggcgc ggcgcggccg gcgggcgtcg cgctttgcgc
     3481 cacgcgcacc ggcggaatgc gcagcacatc gccgtgttgc agccggtggc tggcgtccac
     3541 ccggcctttg ttcacccgca cctcgccgct gcgcaggatg cgatagacgt gacttttggg
     3601 cacgcccttg aggtggcgaa ggagaaaatt gtcaacgcgc tggtcatggc cagcatcgtc
     3661 aactgtcaca aaggtgacgg agtctttacg caagtctgtc attttgctta tactgcgccg
     3721 aaccggggcg ggttgtggat aacccgtgaa tccccggtga ataagttcgg gaaacccaga
     3781 ctcttgtcgc ctcatcgtcc cgacgcgatc acgtcgaggc cacgcgccaa acgcgcccgg
     3841 tccggcatcg tccatgcggc ggcgacacgg ttgtctcgct caccgaaaag cagcgatggt
     3901 aaagcattta cgcgcaacac gcactcacca gaattttcgt tttccggcgt tcaagcccgc
     3961 cagaaaacca tgcagcaaag tttgacccga cgcggtggcc cgatccgacg tgattccatg
     4021 ctcgccccct tgaacaaccg aatgccgggg cgggcattac tgacaccaag acgcgcctgc
     4081 ggcagcaggc ggaacagccg ctttacccgt ggccccgcga aacatacagg aagtcggctg
     4141 aacccattgc cgtaccgctc tttgtgaagg acttcctttc ggtgcgcccc ctgcctgggg
     4201 ctgtcgaaaa cttgctgcgc ccagtgcgcc catttttccg agccgctttg acgccgccac
     4261 tggcggggct gtcagcgcgg attgcctgtt caggcccgtc ttgctgtcgg gcgctgcgcc
     4321 ggactgttgc cggcttgcag tggagaagtg tgcgccgttg gcggccagaa tgccgccaac
     4381 ccctgcgtcg agtgccgcgc aggaccttac taaaatgaaa cgcatgttat tcaatgcgac
     4441 gcaggccgaa gagctgcgcg tcgccattgt cgatggccag aagctgatcg acctcgatat
     4501 cgaaaccgtc ggcaaggagc aaaaaaaggg caatatctac aagggcgtga tcacccgcat
     4561 cgagccctcg ctggaagcct gctttgtcga ttacggcacc gagcgtcatg gttttctgcc
     4621 gttcaaggaa gtctcccgcg cctatttccg tcagtttgac ggcggcggcc gtccgcgcat
     4681 ccaggatgtg ctgcgcgaag gccaggaaat catcgtccag gtagaaaaag acgaacgcgg
     4741 caacaagggc gcggcgctga ccacttacgt cagcctggct ggccgttatc tggtgctgat
     4801 gcccaataac ccgcgtggcg gcggcgtgtc gcgccgcatc gaaggcgaag agcgcaacga
     4861 gctgcgcgag ctgctggccc agctggaaac cccggccggc atgagcctga tcgcccgcac
     4921 cgccggcatt ggccgctcgc tggaagagct gcaatgggat ttgaactacc tgttgcaact
     4981 gtggaccgcg attgaaagcg ccgccggcgc ccagaatgcg ccgttcctga tcttccagga
     5041 aggcagcctg gtgattcgcg ccatccgcga ttacttccag cccgacattg gcgaaatcct
     5101 catcgacacc gaggaaatcc acgcccaggc gcgtcagttc atgagccatg tcatgccggg
     5161 caacgtcaac cgcgtcaagc tgtacaaaga cgacgtgccg ctgttctcgc gcttccagat
     5221 cgaacaccag atcgaaaccg ccttctcgcg cgccgtcacc ctgccgtccg gcggcgccat
     5281 cgtcatcgac cacaccgaag cgctggtgtc ggtggatgtc aactcggcgc gcgccaccaa
     5341 gggcagcgac atcgaaacca ccgcgctgaa caccaacctg gaagccgccg acgaaatcgc
     5401 ccgtcagctg cgtctgcgcg acctgggcgg gttgatcgtg atcgatttca tcgacatgga
     5461 aaacccgaaa aaccagcgcg aagtagaaaa ccgcctgcgc gaagcgctga aaaccgaccg
     5521 cgcccgcgtg caaatgggca agctgtcgcg ctttggcctg ttggagctgt cgcgtcagcg
     5581 cctgcaaccc agcctgggcg aaagcagcca catgccctgc ccgcgctgtc atggcaccgg
     5641 ctttatccgc ggcattgaat cctccgccct gcatatcctg cgcatcattc aagaagaggc
     5701 gatgaaggac aacacttgcg ccgtgcacgc gcaagtgccg gtggacgtgg ccaccttcct
     5761 gctgaacgaa aagcgcgcgg atctgttcac catcgaagcg cgactgaagg tcagcgtggt
     5821 gctgatcccc aatatccatc tggaaacccc gcactacaag atcgtgcgcc tgcgcgccga
     5881 cgatctgccg gaaggcgagg agcccagcta caagatggtg gaaaccccca ccgaagaaaa
     5941 cggctatgcc ggctcccagc cggaaaaagc caaacccgag cgccagcagg ccgccgtgca
     6001 aggcatcacc ccggctcagc cggcgccggt gtccgcccgc gatcaagagc cggcgcccgc
     6061 cccggcagct gcggcgcctg ccgccgccgc cccggcgccg agcccggtgg cggctgcccc
     6121 ggctcagcct ggcgtgatgg cgcgtctggt gggttgggtg aaatcgctgt tcactactga
     6181 agttcccgcc ccggccccgg cgcccgagcc ggtcaagccg gccgccgcca gcccgcgccg
     6241 cgaaggcaat ggccagcgcc gcgaccgtga ccgccgcccg cgcccgccgc gtgacgacag
     6301 cgcgccgcgc cgccgcgacg acaaccgcag cgaacgcgcc gagcgcgccg agcgcgacgc
     6361 cgacgccccg cgtccagaac gcgaaccgcg cgccccgcgc gctgaacgcc aggagcgcgc
     6421 cgagcgtcct gaacgcgccg aacgtgagcc gcgtgaacaa cgcgaaccgc gtgagccgcg
     6481 cgagcgcagc gaacgcccgg aacgcggctc ccgccccgag cgtcaggccc tgcgccatga
     6541 aatgcgcgaa cgcgaagagc gcgaagcccg cgagcaacaa caaggcgaac gcccgaacgc
     6601 cgcgccggcc gatgccgacg cgccgcgcgc agaacgcgcc gagcgcccgg aacgtcgccg
     6661 tcgtcagcct gaagccgcgc cggcagccga agccgtcgag gccgaactga aggccgctga
     6721 agccgtggcg caaaccgccg ctgcccagga cgagcaggcc gatgtcgccc cggccgaagg
     6781 tgaagcccgt gagccgcgca gccgtcgccg tcgtggccgt cgtggtggcg aacgccgtcg
     6841 tgatgaggct caggcggatg gcaaccttga agccgctgcc ccgcagattg accctgaaac
     6901 ccagcatgat gccgtgccgt ctgctgctgc cccggttgcc gctgcggttg ccgtagccgc
     6961 tgctgcggtg gcggtcgaag tggccgctga gacgccgctt gcctctgccg tgcaggaggc
     7021 tgtgccggtg gcagcgccag aggccgagca ggctgctgcg ccggtagcgg tgattccggt
     7081 ggccgtgccg accccgctcg aacccgtggc ggtggcagcg tctgcgcctg tggctgtcga
     7141 gactgtgccg atgcctgagc cgcaagcgca agttgtggtg gacgagccgg tacttgcgcc
     7201 cgccgtgtct gtcgcgccgg ttgagttgcc gcctgtggcc gtcgcagcgg tggtggaggc
     7261 tgttgtggtg gcagatgctg tggcggtcga accggaagtg gctttggcag aacatgccgt
     7321 cgccagcatc gagccggtgg ccgttcagcc tgctgttgag ccagttgctg aggtcgccgt
     7381 gccggccatg ccggaaccgc tggcggcccc ggtgagcatc gccgaagcga ttgcgttggc
     7441 tgagccggtc agtgagcctg ttgtggctga gccgtcggcc gccgagccgg tttctccggt
     7501 cgccgagcct gccgtggcca gtgtcgcgct gggtgggctg gagctggtga gcacccgtgt
     7561 ggctgagccg ttggctgtga tggacgaagc acaagcaccg cgtcgccgtc gtcgtgatgt
     7621 ggctgaatcg ctggcggccg gcaccgagca agctgctttg cagcaggtgg aaacgttgaa
     7681 tctgcctgcc gctgtgccgc aagctgctgc cagcttggca acggctcagc ctgtgcgccg
     7741 tcgtgatcgt ctggcggctc agtctggcgt tgaggcggtt gagatggttc aggtgcaaac
     7801 ccgcgattga tcgcgctcac ggggcggttg gatgtgcttg tccaactgcc ccgctaaaaa
     7861 gtgcagaaag tgcttgacct ggctttctaa gatcgttatt atctctttct cgattcctcg
     7921 atagctcagt cggtagagcg acggactgtt aatccgcagg tccctggttc gagcccaggt
     7981 cgaggagcca gattcagcaa aaaagccagc acagatgtgc tggctttttt gtttttgtgc
     8041 acaaattgct gatgctaagt atttttgcgg atagcctctg gtatattcat gacatctgtg
     8101 tgatccggga accaatatgc gcggtttgtg gcgcgctgtg caggcgtgtg gggtggtggc
     8161 gttgctggca gtggccggtg tggcgtgtga gcaacgtaat ccggtgttta tcggtatctg
     8221 gatgtgtggc gatatggagc tgcaattcag tggcgatggg cgctttcgtt ggcgtggcgt
     8281 agatggcgat gagttgaacg gcaattttca tctggtgcgc gacggtagtg cttatcgcat
     8341 tgactatcag ccggtgcgct gggcggtgca ggaaaagcgt tatattccca ctcgctatcc
     8401 ggtagatggc ttttatctga gtgccacccc gtctggtgag ggtggtgaca tgctggcctg
     8461 tcagcgcaag cgtgtgcgtg gtgctgcgcg ggtgttgtcc tggctggacg aagggccctg
     8521 agtggattgg tgttttttgg ggtttgctgt tttggtttgg tttgaatgca aaaaagccca
     8581 gtctgttgac tgggctttta atgcttgggg tggatgatgg gactcgaacc cacgacagcc
     8641 ggaatcacaa tccgggactc taccaactga gctacaccca ccgctgaaac tgtaatgatc
     8701 ttgctgaata cagtgcaaaa tcagaaaaac tgtcttgttt gactggcgcg cccgacagga
     8761 atcgaacctg taaccgccgg cttagaaggc cgatgctcta tccggttgag ctacgggcgc
     8821 tcaactgtcg gttgcaactg gtcggggtgg tgggattcga actcacgacc ccctgatccc
     8881 aaatcaggtg cgctaaccag gctgcgctac accccgaaca agagaacagg actataccca
     8941 ggctttggca ggctgtcaag ctttgacttg ataaaatcgc tcagccagcc atttgcgttg
     9001 agcgcagcgc ctggcgtgtc agcatttgcc gggtgcgtgg cggtcaatgg ttgagccgtg
     9061 caggccaaca atgcgaaaat cccggttttg tttacgaaca tcttgactgg aaagacccaa
     9121 caccatggcg gctcagctta ttgacggcaa aaccatttcc gaagccctgc tcaaccgtgt
     9181 caaagccggc gtggacgccc ggctggccga gggccgtcgc gcacccgcgc tggccgtggt
     9241 gctggtgggc aacaacccgg cctcggaaat ctatgtgcgc aacaaaaagc gctcgtgcga
     9301 aaaagtcggc atccgctcgg tggcgtttga tctgccggcc agcaccactc aggctgaact
     9361 gctggccatt gttgaccagg tcaacgccga cccggaaatc gacggcattc tggtgcaact
     9421 gccgctgccg gcgcatatcg acgccgatgc ggtgattgaa cgcatcgacc cgaaaaaaga
     9481 cgtggacggc tttcacccct acaacatcgg ccgcctggcg ctgaaaatgc cgctgctgcg
     9541 cccgtgcacc ccccgcgggg tgatgaccat gctggaaatg tccggcatcg accccaaggg
     9601 caagcacgcg gtgatcattg gcgcatccaa tattgtcggc cggccgatgg cgctggagct
     9661 gttgctggcg cgcgccaccg tcaccgtctg ccacagcgcc accgctgacc tggccgccga
     9721 agtgcgtcgc gccgatatcg tggtggcggc ggtgggcaag ccgggctttg tgccgggcga
     9781 ctggatcaag gaaggcgccg tggtggtgga cgtgggcatc aaccgtctgc cggatggcaa
     9841 ggtggtgggc gatgtggact ttgccgccgc cagcgagcgc gccagctgga tcactccggt
     9901 gccgggcggc gtgggcctga tgacggtggc caccctgctg caaaacaccc tggactcggc
     9961 caatctgcac gcctgagccg ttttttgacc gattgctgac gaaaaaccta aatcatgtct
    10021 gagcgcaact ccgcaaccct gctgatcagt tgtcctgacc gcaaggggct ggtggccacc
    10081 atcgccaact ttttgatggc ctacaacgcc aacatcctgc acgccgacca gcaccaggat
    10141 tccagcgaaa acctgtttct gatgcgcgtc gaatgggacc tgagcggctt taccctgccg
    10201 atggaaaact tcgccgccgc gtttcagccg attgccgacg aaaaccagat gacctggcgg
    10261 gtcaccctgt ccagcaaaaa accgcgcatg gcgctgtttg tctccaaata cgaacactgc
    10321 ctggtggacc tgatgcaccg ctggcgcatt ggcgaactga actgcgacat cgccctggtg
    10381 atttccaacc acgaagactg ccgccgtctg gtggaattta acggtattcc cttccatgtc
    10441 gtgccggtca cccgcgacaa caaagccgaa atggaagccc gccagtgggc gctgctggaa
    10501 gaagcccagg tggatttcat cgtgctggcg cgctatatgc aagtgctgtc gccgtccttt
    10561 gtcgagcgct atccgagccg ggtgatcaat atccatcact ccttcctgcc ggcctttgac
    10621 ggcgccaagc cctatcaccg cgcctttgcc cgtggcgtga agctgattgg cgccaccagc
    10681 cactacgtca ccgaagtgct ggacgacggc ccgatcatcg agcaggaagt cacccgcatc
    10741 tcgcaccgcg acgacgtgga agacctggtg caaaaaggcc gcgacctgga aaaagtcgtg
    10801 ttgtcgcgcg ccgtgcgctg gcatatcgaa aaccgcatcc tggcctacgc caacaaaacc
    10861 gtcatcttcg actgaccccg ccccacgtcc gccgcgcacg cggcggacgc ctgcctgaga
    10921 ctctcccatg cgcctgattg atgacttgat cgagctggcc tgcctgcgcg cccgcccgct
    10981 ggccgaatac cgccatccag cttggcagtt gctgtgcgcc ttattgattt tgtcggtgat
    11041 caacagcgcc agcgcagaaa actttaccgg ccccttgccg gcgcggctgg ggttttttgc
    11101 cgtgtttgcg ctgctggaaa gtgtgctggt caccgccgtg ctgacgctat ggctgcggct
    11161 gagcggctgg cgcgagcggg attccctgtg gccgctggtg gtgctgtcca gcggcgtgca
    11221 ggcgctggag ccgctgacca gctggctggg cgaggccgcc gggcaaacgc tcagcggact
    11281 cttggcggtg tattcggtat tggtgctggt gttgggcgtg gcgcgcgcca ccggcatgcc
    11341 cgcctggcgg gtggccgccg gcgtggtgat gttcctgccg ctgggcgcta tgctgttggc
    11401 ggtgatgctg ggcgcggcgg cggatatggg ttgggtgacg ctgccggtgg cgcctgctga
    11461 cgcgaattag agccgctcac aaaaccctcc tggctttgtt gtacggcctt ggcgcgctac
    11521 ccgcgctgtc tgcgattctg gcggacggtg caaggcgtat ggccaaaccg cgcgacaaca
    11581 ccaggcgggt tgggtgagcg gtgcttgcca atgcatcaag ccaaatcgag atatgaacat
    11641 gggctgtgcg ctcgtgagct attgctgacg gtaatttgaa cgacaaagtc gatgcaggca
    11701 gcgtcactgg gctggtgggc cgcccgcgaa gagatcagca agggatgcgc tgaaaaaagt
    11761 gacttctgaa ggaatggtgg cttaattttt tgattttgcc gtatttttta tgggtgcgat
    11821 ttttctgaat ggctcagtgc atcccaagcg tactggccga ttggcaaaac acgtaaagtg
    11881 catcacgccc gacggttaaa tgcatgttct tcttcgtgaa tttctctcat ggtcggcgcc
    11941 ggcttgttgt agtcgtaata actggccacc gcgcggctgc ccagggctgc ggcaacgcca
    12001 ttgcgcatgg cagcacggta ttcatgctca cttaaacctt tggcggatgc ggccaaagca
    12061 gataggtcga tattggcatg tgaataggct gagccatcct tatacaccac cgccaaaata
    12121 ttgcccgccg ggccacggaa aatactgtca gcctgcattt gcagttgctt ttcaagctcg
    12181 gccgcagagg ggcgatgctc tctggtttcc ggtcccatta atgccatggt tttttccatg
    12241 tctttcttat gcttactcag cgcctcctcg ccttttagcc ctgtcataat ttcagcatag
    12301 cgcagctcga gttcacgact tttttctgca ctggtcgtaa atgcggtcca agtaacagat
    12361 gtacattctt gtccttctgg cgtgctgatc gatttgccgc ttgacttact gttgaggggc
    12421 aaagtggtcg aagcattggt attgatttgc atattacctc catagggtca gggtaaacag
    12481 gtagctccaa ataaaatgct gcctgctgac ggttacacta cgccaaaata atcagatagg
    12541 ctgagaatat aatcttgggt ttccaaatat tcaatagtgt aaattccacc caggaaaaag
    12601 ttggagatga ccaccccctc ggtgatttga ccatcactgg catcggccat actgctgatc
    12661 cacagatcat ttgcttgccg taaaggaatc agttggtcaa gtgtcacatt ggggatatac
    12721 agcttatcga ttccgccacc ataaccaggg gtgtagctgg cagtcacacc atcattgatg
    12781 gtgtcctggc cattgccata gccgatatat tgcacataaa gatcatcacc aaagccgccc
    12841 ag
//
LOCUS       DAOPYX010000043        12828 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_5404_length_12828_cov_12.776638, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000043 DAOPYX010000000
VERSION     DAOPYX010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 12828)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12828)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12828
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5404_length_12828_cov_12.776638"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(2..1183)
                     /locus_tag="PKN55_03680"
     CDS             complement(2..1183)
                     /locus_tag="PKN55_03680"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008954531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HNX70375.1"
                     /translation="MQDPVVIVGMARTPMGGLQGSLSSLTAAQLGAAAVAAAVARAGV
                     AGEDIDEALMGCVLPAGQGQAPARQAVLGAGLPQSVPCSTVNKMCGSGMKAAMLAHDV
                     LLAGASRVVLAGGMESMSNAPYLLAKARAGFRLGHGEIKDHMFLDGLEDAYQKGALMG
                     VFAEQCAEHYGFTRAMQDEFAIRSLTRAQDAIRAGRFASEITPVTLQGRKGEEVVSDD
                     EQPLKANLDKIPQLKPAFKKDGTVTPANASSISDGAAALVLMRASEAERRGLTPLARI
                     VGHSSHAQAPAWFTTAPTFAIEKLLGRIGWSADSVDLYEINEAFAVVTLAAMHDLKLP
                     AEKVNVHGGACALGHPIGASGARIVVTLLSALQQYGLRRGVASLCIGGGEATALAIER
                     M"
     gene            complement(1315..1719)
                     /locus_tag="PKN55_03685"
     CDS             complement(1315..1719)
                     /locus_tag="PKN55_03685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018151881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HNX70376.1"
                     /translation="MSTVQDRTFTIGELAREFDITPRAIRHYEDQGLLEPERRGQHRI
                     YSRRDRVRLMLTLRGKRIGLTLQEIRELFELYDTANDDESQLFEFIKLLSRRESQLRQ
                     QQEDINAVLKEVGSLRVQCERLLEKRTGGIPE"
     assembly_gap    1800..1899
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1968..2822
                     /locus_tag="PKN55_03690"
     CDS             1968..2822
                     /locus_tag="PKN55_03690"
                     /EC_number="1.1.1.157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014087833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HNX70377.1"
                     /translation="MQIQHIGVVGAGQMGSGIAQVCAQAGLTVTLVDVHQAALSRARD
                     TIARSLARLVKKGALTQVDADASQARIAYHDAPTELAHCQLVIEAATENWEIKAQLFA
                     RIASVVNEQTILASNTSSISLTRLAARVPAAHRVIGMHFMNPVPVMPLVEIIRARQTD
                     DATFAAIRALSERLGKTPVSVNDAPGFVSNRVLMPMINEAIFCLHEGIASAGDIDAVM
                     KLGMNHPIGPLALADLIGLDTCLAIMDVLYHELRDSKYRACPLLVQMVDAGQLGRKTA
                     KGFYGYES"
     gene            3207..5186
                     /locus_tag="PKN55_03695"
     CDS             3207..5186
                     /locus_tag="PKN55_03695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012695700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNX70378.1"
                     /translation="MASPQALQVNAHAPSRPALDTFPKLLLNHAAVRGNRPAMREKDL
                     GIWQTWSWAEVAAEVRAIACGLAARGFARGDRLAIIGDNRPRLYWSMAAAQCLGGVPV
                     PLYQDAVAEEMVFVLQDAGARFAVVEDQEQVDKLLEIRDRLPTLTHIIYDDPRGMRHY
                     QHDGLHSFAEVLGDGRVHHDEHPHFFQSEVDGVGADDIAIMLYTSGTTGNPKGVAHTY
                     HSMIVTARNSIEAEGLSDQEEVLAYLPMAWVGDNLFSYAQSMVAGFCVSCPESSETVM
                     TDMREIGPTYYFAPPRVYENLLTQVTIRMEDASPLKRWMFNYFMEHARKTGTRIVDGQ
                     PVSMIDRLGYWLGNLLVYGPVKNVLGFSRIKLAFTAGEAIGPELFDFYRALNINIKQL
                     YGSTEAYVMVCVQPNGQVKPDTVGTPAPGVEVKIADSGEVLFRGPGVFHSYWNRPDAT
                     AETKTDDGWVLTGDAGFFDDDGHLKIIDRAKDVGKFTQGGMFAPKYLENKLKFFPYIK
                     EAVTFGDGRDHATAFINIDLSAVGNWAEKRNLPYTGYTDLAGQAPVYELIRECVEKIN
                     ADLARDPRLGHSQIKRFLILHKELDADDGELTRTRKVRRKFISERYGVLVEALYSSQE
                     SCMIDAQVKFEDGRVGNIRATLKLCDVQTYPLATA"
     gene            5202..5990
                     /locus_tag="PKN55_03700"
     CDS             5202..5990
                     /locus_tag="PKN55_03700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012695701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNX70379.1"
                     /translation="MNPHRKIGDVILKAENISLSFGGVKALTNISLDVREHEICAIIG
                     PNGAGKSSMLNVINGVYHPQEGRVSYKGHVRDSMHAHEAARHGIARTFQNIALFKGMS
                     VLDNIMTGRNLKMHTNFLQQALYWGATQREEIRHRRKVEEIIDFLQIENIRKTPVGRL
                     PYGLQKRVELGRALAAEPTLLLLDEPMAGMNVEEKQDMCRFILDVNDEFGTTIVLIEH
                     DMGVVMDISDRVIVLDYGKKIGDGSPEEVRANPDVIAAYLGTSH"
     gene            6012..6893
                     /locus_tag="PKN55_03705"
     CDS             6012..6893
                     /locus_tag="PKN55_03705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012695702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HNX70380.1"
                     /translation="MQFFIEVMVGGLLAGVMYSLVALGFVLIYKASGVFNFAQGALVL
                     FAALTFVSIEKMGVPFWIALAITLGIMVLMGVAIERIVLRRLVNQPPISLFMATIGLS
                     FFIEGLAQTVWGTEVHGLELGITDEPFDVMGVFISKFDLFAATLSGVLVAGLALFFQK
                     TKLGRALRAVADDHQAALSVGIPLKHIWAIVWAVAGFVALVAGMLWGSRLGVQFALTL
                     VVLKALPVLILGGFTSVPGAIVGGLIVGAGEKLAEVYLGPFIGGGIENWFPYMLALVF
                     LLVRPEGLFGERHIERV"
     gene            6947..8011
                     /locus_tag="PKN55_03710"
     CDS             6947..8011
                     /locus_tag="PKN55_03710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012695703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HNX70381.1"
                     /translation="MIYREAGQFKINYQSDSAILPIVQDRMFMIGLMVVSFLVIPVLG
                     SDYFLAGILTPFLILSLAALGLNILTGYAGQLSLGSAAFMAVGAFATYNFMIRIPGIP
                     LLLALVLGGLMAALVGILFGLPSLRIKGFYLAVATLAAQFFIEWALTKFGWFTNYSSS
                     GVISAPEMQVFGIVIDSAAKKYLFTLAIVALLTLAAKNMVRSSLGRSWMAVRDMDVAA
                     EVIGIPILKTKLLAFAISSFYCGIAGALWAFVLLGTVDPQAFDLHRSFQVLFMIIVGG
                     VGSILGSYLGAAFIVLLPIVLNLIGQLFHGAVSTQFLSNMEMMLFGGLIIFFLIVEPL
                     GLARLWQIAKEKLRMWPFPH"
     gene            8227..9555
                     /locus_tag="PKN55_03715"
     CDS             8227..9555
                     /locus_tag="PKN55_03715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018747578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNX70382.1"
                     /translation="MKTNSLKRSVLAVAVSLGTLAAGQAWAAEQFIPIPSYRVGPYAS
                     GGSKYYGGLIDYINLVNAKEGGVGGVKLTYEECETEYKNDRGVECYERLKAKGEAGAS
                     MFNFMSTGITYATMDRASQDKIPLVTIGFGRADAKDGSVFPYVFPLITSYWSQASAKI
                     SYIGQRLGGMDKLKGKKIVNLYHGSAYGKETMPVLEAFAKKYGFELVNVEVPAPGTEQ
                     QSQWLEIRRQQPDFVILRSWGVMTPAAIKAAAKVGFPRDKIVAVWWGGSEEDVIPAGE
                     AAKGYIAGSFASDGVGFPVIQDIQKTLYANGKGNLSDQSAIGTVNYNRGVIGGIMMVE
                     AIRVAQGKFGKKALNGDEVRWGLEHLDLSPARLKALGAANMIPGVKTSCANHEGSGYT
                     RFMQWDGKAWKPASGWIAPDNSVLDPLVKASAEKYAKEKGITPRDCSKEK"
     gene            9640..10488
                     /locus_tag="PKN55_03720"
     CDS             9640..10488
                     /locus_tag="PKN55_03720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012695705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNX70383.1"
                     /translation="MSQTGIVPGHTDQQPSPFLLSVNNIEVIYNHVILVLKGVSLDVP
                     EGSIVALLGGNGAGKSTTLKAISNLLASERGDVTKGSVEFRGERVDQLTPDALVKRGV
                     VQVMEGRHCFGHLTVEENLLTGAYTRKASRAEIAQDLEKVYHYFPRLKVRRQSQAGYT
                     SGGEQQMVAVGRALMARPSMILLDEPSMGLAPQIVEEIFEIVKDLNQKERVSFLLAEQ
                     NTMVALRYANFGYILENGRVVMDGAADELASNEDVKEFYLGLGGDGRKSFREVKHYRR
                     RKRWLA"
     gene            11060..12295
                     /locus_tag="PKN55_03725"
     CDS             11060..12295
                     /locus_tag="PKN55_03725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012695706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNX70384.1"
                     /translation="MPHLDALETRPHAQRVAEQYARLPAQLAYAREHSPAYAQALAGL
                     DLSAVTDAQALAGLPVTRKSALIEQQQACPPFGGYAAMAAGAASRVFASPGPIYEPQG
                     PGEDFWRMSRALYAAGFRSGDIVHNTFSYHFTPGGFMLDSGARALGCAVFPAGVGQTE
                     QQVMALRDIGATGYTGTPSFLKIILDKADELGIALPRLNKALVTGEALPNSLRQQFEA
                     RGIQVRQCYATADLGLIAYESQPGEGLVVDEGIVLELVKPGTGELVADGDVGEVVVTP
                     LHNRAYPLVRFATGDLSAFLDSAGSCGRSNRRIKGWLGRADQTAKIKGMFVHPAQIHA
                     IGPRHPALGRLRLVVERVNDLDHMTLHAESADSDAALRQAVADSLRELTKLKGEVCLC
                     APGSLANDGKIIDDQRPLA"
     gene            12381..>12828
                     /locus_tag="PKN55_03730"
     CDS             12381..>12828
                     /locus_tag="PKN55_03730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014087834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNX70385.1"
                     /translation="MTATLPTVPLLIQGEFVESATTQWRDVVNPATQEPLARVPFATA
                     DEINAAIASAKAAFKTWRKTPIGARARIFLKLQQLIRENMADLAATLTAEQGKTLADA
                     EGDVFRGLEVVEHAANIGTLQMGEFAENVASGVDTYTLLQPLGVCAG"
BASE COUNT         2314 a         3956 c         4076 g         2382 t
ORIGIN      
        1 cttacatccg ctcaatcgcc aacgccgtcg cctccccgcc gccaatgcac aaactcgcca
       61 ccccacggcg caaaccatac tgctgcaaag ccgacaacaa cgtcaccaca atccgcgccc
      121 ccgacgcgcc aatcggatgg cccagcgcac acgcgccgcc atgaacattc accttctccg
      181 ccggcaactt caaatcatgc atcgccgcca gcgtcaccac ggcaaacgcc tcgttgattt
      241 catacaaatc cacactgtcg gcgctccagc caatccgccc cagcaacttc tcgatggcga
      301 aggtcggcgc cgtggtgaac caggccgggg cctgggcgtg gctgctgtgg cccacaatcc
      361 gcgccagcgg ggtcaagccg cgccgctcgg cctcgctggc gcgcatcaac accaaggcgg
      421 cggcgccgtc agaaatcgag ctggcgttgg ccggggtcac cgtgccgtct ttcttgaacg
      481 ccggcttgag ctgggggatt ttgtccagat tggccttcag cggttgctca tcgtcgctga
      541 cgacttcttc gcccttgcgg ccttgcaggg tcaccggggt gatttcgctg gcaaagcggc
      601 cggcgcgaat ggcgtcttgc gcgcgggtca gcgagcggat ggcgaattcg tcctgcatgg
      661 cgcgggtaaa gccatagtgc tcggcgcact gctcggcaaa cacccccatc agcgcgcctt
      721 tttggtaggc gtcttccagg ccgtcaagaa acatatggtc tttgatttcg ccgtggccca
      781 gccgaaagcc ggcgcgcgcc ttggccagca gatagggggc gttgctcatg ctttccatgc
      841 cgccggccag caccacccgg ctggcgccgg ccagcaacac atcatgcgcc agcatggcgg
      901 ccttcatgcc cgatccgcac atcttgttca ccgtgctgca cggcaccgac tgcggcaggc
      961 cggcgcccag caccgcctgg cgcgccgggg cctggccctg gccggcgggc agcacgcagc
     1021 ccatcagcgc ctcgtcgatg tcttcgccgg ccacgccagc gcgcgccacg gcggcggcca
     1081 cggcggcggc gcccagctgg gcggcggtga ggctggacaa gctgccttgc aggccgccca
     1141 tcggggtgcg cgccatgccg acaatcacta ccggatcttg catgtgtctc tccttgtttt
     1201 tttgccgccg ggtgtgccgg cgtgtgcgtg gccgcgtgcg tcggcgctgg cgcgccgcgc
     1261 gcatcggtaa aattacctat cgtttacgtt aacgtaaacc aaccggcaaa aaactcactc
     1321 ggggatgccc ccggtgcgtt tttccaacaa acgctcgcac tgcacccgca aggagccgac
     1381 ttccttgagc accgcattga tgtcttcctg ctgctgacgc agctggcttt cgcggcgcga
     1441 gagcagcttg atgaactcga acaactgcga ctcgtcatcg ttggcggtgt cgtagagttc
     1501 gaacagttcg cggatttcct gcaaggtgag cccgatgcgc ttgccgcgca gcgtcagcat
     1561 caaacgcacg cggtcgcggc gcgaatagat gcggtgctgg ccgcgccgtt cgggctccag
     1621 caagccttgg tcttcgtagt gacgaatcgc ccggggggtg atgtcgaact cgcgcgccag
     1681 ctcgccgatg gtgaaggttc tgtcctgaac tgtgctcaca atgctgcaac tcgtagggat
     1741 atttcgggtt aagcggctga ttttaccgcg tttcacccca tcacggccac cctggcggcn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt tgttgacgtt tacgtaaacg
     1921 tgatagtagt ttaaccacac ctcaacacgc aaggcaagga gagcagcatg caaattcaac
     1981 acattggcgt agtcggcgcc gggcagatgg gcagcggcat tgcccaggtg tgcgcccagg
     2041 ccgggctgac ggtgacgctg gtggacgtgc accaggcggc gctgtcgcgc gcgcgcgaca
     2101 ccatcgcccg cagcctggcg cggctggtga aaaaaggcgc gctgacccag gtcgacgccg
     2161 acgcatccca ggcgcgcatc gcctaccacg atgcgcctac cgagctggcg cactgccagc
     2221 tggtgatcga ggccgccacc gaaaactggg agatcaaagc ccagctgttt gcccgcattg
     2281 ccagcgtggt caacgaacaa accattttgg cgtcaaacac ttcgtcgatt tcgctcaccc
     2341 gcctggccgc gcgcgtgccg gcggcgcatc gggtgattgg catgcacttc atgaacccgg
     2401 tgccggtgat gccgctggtg gaaatcatcc gcgcccgcca gaccgacgac gccacctttg
     2461 ccgccatccg cgcgctgagc gagcggctgg gcaagacgcc ggtcagcgtc aacgatgcgc
     2521 cgggctttgt ctctaaccgg gtgctgatgc cgatgatcaa cgaggcgatt ttttgcctgc
     2581 acgagggcat tgccagcgcc ggcgacatcg acgcggtgat gaagctgggc atgaaccacc
     2641 cgattggccc gctggcgctg gccgacctga ttggcctgga cacctgcctg gcgatcatgg
     2701 acgtgctcta ccacgaattg cgcgacagca aataccgcgc gtgtccgctg ctggtgcaga
     2761 tggtggacgc cggccaattg ggccgtaaga cggctaaggg tttttacggt tatgaatcat
     2821 aggcttgatc ttgtcatgca ctgctgcggt gcaacatcga tagttgttgc atgacattga
     2881 ccgcttcggc tagtctagtt ccggcaagtt tacgttaacg tcaacgtaat gaaatccgat
     2941 tcagcttgcc ggcgcattcc gaggcggcca ccgcccaccc gcgccacatg acccggcgcc
     3001 acgacgccgg gtcatgattt gtcgccagag accgggccca cccggcgcgc gacaccctga
     3061 accggctgtg cgctggcgcg cagcctgaac agattcagag ccgctcgccc cgctgcatgc
     3121 gcggcccagg ccgttgacac ggtcaggccg ccgcgccagg caaagcgcgc ggttcgttag
     3181 agaacatgag gacggagaca acacacatgg caagcccgca agcgttgcag gtcaacgcgc
     3241 acgcgccatc caggccggcg ctggacacct tccccaagct gctgctcaac catgccgccg
     3301 tgcgcggcaa tcgtcccgcc atgcgggaaa aagacctggg catctggcaa acctggagct
     3361 gggccgaggt ggccgccgag gtgcgcgcca ttgcctgcgg cctggccgcg cgcggctttg
     3421 cccgtggcga ccgcctggcc atcatcggcg acaaccgccc gcgcctgtac tggagcatgg
     3481 ccgccgccca gtgtctgggc ggcgtgccgg tgccgctgta tcaggatgcg gtggcggaag
     3541 aaatggtgtt tgtgctgcaa gacgccggcg cgcgctttgc cgtggtggag gatcaggagc
     3601 aggtggacaa gctcctggaa atccgcgacc gcctgccgac gctcacccac atcatttacg
     3661 acgacccgcg cggcatgcgc cactaccagc atgacggcct gcacagcttt gccgaagtgc
     3721 tgggcgatgg ccgcgtgcac cacgacgagc acccgcattt tttccagagc gaagtggacg
     3781 gcgtgggcgc cgacgacatc gccatcatgc tgtacacctc gggcaccacc ggcaacccca
     3841 agggcgtggc gcacacctac cacagcatga ttgtcaccgc gcgcaacagc atcgaggcgg
     3901 aagggctgag cgaccaggaa gaagtgctgg cctatctgcc gatggcctgg gtgggcgaca
     3961 acctgttttc ttatgcgcag tcgatggtgg ccgggttttg cgtgtcctgc ccggaatcca
     4021 gcgagacggt gatgaccgat atgcgcgaaa tcgggccgac gtattacttt gccccgccgc
     4081 gcgtctacga aaacctgctg acccaggtga ccatccgcat ggaagacgcc agcccgctca
     4141 agcgctggat gttcaattac ttcatggagc atgcgcgcaa gaccggcacc cgcattgtcg
     4201 atggccagcc ggtgagcatg atcgatcgtc tgggctactg gctgggcaat ctcttggtgt
     4261 atggcccggt gaaaaatgtg ctgggcttct cgcgcatcaa gctggcgttt actgcgggcg
     4321 aggccatcgg cccggagctg tttgattttt atcgcgcgct gaatatcaac atcaagcagc
     4381 tgtacggctc gaccgaggcc tatgtgatgg tgtgcgtgca gcccaatggc caggtgaagc
     4441 cggacacggt gggcacgccg gcgccagggg tggaggtcaa gatcgccgat tctggcgagg
     4501 tgctgttccg tggccctggc gtgttccaca gctactggaa ccgcccggac gccaccgccg
     4561 aaaccaagac cgacgatggc tgggtgctga ccggcgatgc cgggttcttt gacgacgacg
     4621 gccacctgaa gatcatcgac cgcgccaagg atgtgggcaa gttcacccag ggcggcatgt
     4681 ttgcgccgaa gtacctggaa aacaagctga agttcttccc gtatatcaaa gaggcggtca
     4741 cttttggcga tggccgcgac cacgccacgg cgttcatcaa tattgacctc tctgcggtgg
     4801 gcaactgggc ggaaaaacgc aatctgccct acaccggcta caccgacctg gccggccagg
     4861 ccccggtgta cgagctgatc cgcgaatgcg tggaaaagat caacgccgat ctggcgcgcg
     4921 acccgcgtct gggccactcg cagatcaagc gctttttgat tttgcataaa gagctggacg
     4981 ccgacgacgg cgagctgacc cgcacccgca aggtgcgccg caaattcatt tccgagcgtt
     5041 atggcgtgct ggtcgaggcg ctgtattcca gccaggagag ctgcatgatc gacgcccagg
     5101 tgaagttcga ggatggccgc gtgggcaata tccgcgccac gctcaaactg tgtgatgtgc
     5161 agacctatcc cctggccacg gcataacgga gaagcgagcc catgaatcct catcgcaaaa
     5221 ttggcgacgt catcctcaag gcagaaaaca tttcgctgtc gtttggcggg gtgaaggcgc
     5281 tgaccaatat cagcctggat gtgcgcgagc atgaaatctg cgccatcatc ggccccaacg
     5341 gcgccggcaa gagctcgatg ctcaatgtca tcaacggcgt ctatcacccg caagaaggcc
     5401 gcgtcagcta caagggccat gtgcgcgaca gcatgcacgc ccacgaagcc gcccgtcacg
     5461 gcattgcccg cacttttcag aacatcgcct tgttcaaggg catgagcgtg ctggacaaca
     5521 tcatgaccgg gcgcaacctc aagatgcaca ccaacttcct ccagcaggcg ctgtactggg
     5581 gcgccaccca gcgcgaggaa atccgccacc gccgcaaggt ggaagaaatc atcgactttc
     5641 tgcaaatcga gaacatccgc aaaaccccgg tcggccgcct gccctatggc ctgcaaaagc
     5701 gcgtcgagct gggccgcgcg ctggccgccg aacccacctt gctgctgctg gacgagccga
     5761 tggccggcat gaacgtcgaa gaaaaacagg atatgtgccg cttcatcctc gacgtcaacg
     5821 acgagttcgg caccaccatc gtgctgatcg aacacgatat gggcgtggtg atggatattt
     5881 ccgaccgggt gatcgtgctg gactacggca agaaaattgg cgacggcagc cccgaggaag
     5941 tgcgcgccaa cccggatgtg atcgccgcct atctgggcac gtcccactag ccggccaggg
     6001 agagacaaat catgcagttt tttattgaag tgatggtcgg cggcctgctc gccggggtga
     6061 tgtattcact ggtggcgctg ggctttgtgc tgatttacaa ggcttccggc gtgttcaact
     6121 ttgcccaggg cgcgctggtg ctgtttgccg cgctgacctt tgtcagcatc gaaaaaatgg
     6181 gcgtgccgtt ctggatcgcc ctggccatca ccctgggcat catggtgctg atgggggtgg
     6241 cgattgagcg gattgtgttg cgccggttgg tcaaccagcc gccgatttcg ctgttcatgg
     6301 ccaccatcgg cttgtccttc ttcatcgaag ggctggcgca aacggtgtgg ggcaccgagg
     6361 tgcatggcct ggagctgggc atcaccgacg agccgtttga cgtgatgggc gtgtttatct
     6421 ccaagtttga cctgttcgcc gccacgctgt ccggcgtgct ggtggccggt ctggcgctgt
     6481 tcttccagaa aaccaagctg ggccgcgcgc tgcgcgcggt ggccgatgac catcaggcgg
     6541 cgctgtcggt gggcattccg ctcaagcaca tctgggcgat tgtctgggcg gtggccggtt
     6601 ttgtcgccct ggtggccggc atgctgtggg gctcgcggct gggcgtgcag ttcgccctca
     6661 ccctggtggt gctcaaggcc ttgccggtgc tgattctggg cggcttcacc tcggtgccgg
     6721 gcgccattgt cggcgggctg attgtcggcg cgggcgaaaa gctggccgaa gtctacctgg
     6781 gcccgtttat cggcggcggc atcgaaaact ggttccctta catgctggcg ctggtgtttc
     6841 tgctggtgcg ccccgagggc ttgtttggcg agcgccatat cgagcgagtg taaagcctgc
     6901 ttgatgtggc gctgacatca cgcctgacat tctggagaaa actgcaatga tctaccgcga
     6961 agctggacag ttcaaaatca attaccagag cgattcggcc attttgccca tcgttcagga
     7021 ccgcatgttc atgatcggcc tgatggtcgt gagctttctg gtcatcccgg tgctgggctc
     7081 ggattacttt ctggccggca tcctcacccc gtttctgatc ctgtcgctgg cggcgctggg
     7141 cctgaacatc ctcaccggct acgccggcca gctgtcgctg ggctcggcgg cgttcatggc
     7201 ggtgggggcc ttcgccacgt acaacttcat gatccgcatc cccggcattc cgctgttgct
     7261 ggccctggtg ctgggcgggc tgatggcggc gctggtgggc attttgtttg gcctgcccag
     7321 cctgcgcatc aagggctttt atctggcggt ggccaccctg gcggcgcagt tctttattga
     7381 atgggcgctg accaagtttg gctggttcac caattattcc agctcggggg tgatttccgc
     7441 gccggaaatg caggtgttcg gcatcgtcat cgactccgcc gccaagaaat acctgttcac
     7501 cctggccatt gtcgccctgc tcaccctggc ggccaaaaat atggtgcgca gctcgctggg
     7561 ccgcagctgg atggcggtgc gcgacatgga cgtggccgcc gaagtgatcg gcatccccat
     7621 cctgaaaacc aaactgctgg cctttgccat cagctcgttc tactgcggca ttgccggcgc
     7681 gctgtgggcc tttgtgctgc tgggcacggt ggacccgcaa gcctttgacc tgcaccgctc
     7741 gtttcaggtg ctgttcatga tcattgtcgg cggcgtgggc tcgattctcg gttcttacct
     7801 gggcgcggcg ttcatcgtgc tgctgcccat cgtgctgaac ctgattggcc agctgttcca
     7861 cggcgcggtc agcacccagt ttctgtccaa tatggaaatg atgctgtttg gcggcctgat
     7921 tattttcttc ctgattgtcg agccgctggg cctggcccgc ttgtggcaga tcgccaagga
     7981 gaagctgcgc atgtggccgt tcccgcactg agcgcggctc acaaaaccct cctggcgtgg
     8041 ttgcacgacc ttgccgtact agccgtctac ggtcgtgcgc ctagccaggg ccgcttcgct
     8101 gagtttggtg agcgacgctg aagcacggat gcctgcatcc atcccttgtg tagtcccgcc
     8161 atgccgtcgc gcagacggcg gtgtggcaaa gccgtagctg acattcaaaa accgaggaga
     8221 gacaccatga agacgaattc cctgaagcgt tccgtcctgg ccgtggccgt gtcgttgggc
     8281 acgctggccg caggccaggc ctgggccgcc gagcagttca tcccgattcc cagctatcgc
     8341 gtcggcccct acgcctcggg cggctccaag tattacggcg gcctgattga ctacatcaac
     8401 ctggtcaacg ccaaagaggg cggcgtcggc ggcgtcaagc tcacctacga agagtgcgaa
     8461 accgaataca aaaacgaccg tggcgtagag tgctacgagc ggctcaaggc caagggcgaa
     8521 gccggcgcct cgatgttcaa cttcatgtcc accggcatca cctacgccac catggaccgc
     8581 gcctcgcagg acaaaatccc gctggtgacc atcggctttg gccgcgccga cgccaaggac
     8641 ggcagcgtgt tcccgtatgt gttcccgctg atcacctcgt actggagcca ggcctcggcc
     8701 aagatcagct atatcggcca gcgcctgggc ggcatggaca agctcaaggg caaaaagatc
     8761 gtcaacctct accacggctc ggcttacggc aaagaaacca tgccggtgct ggaggcgttc
     8821 gccaagaaat acggctttga gctggtgaat gtggaagtcc cggcgccggg caccgagcag
     8881 caatcgcaat ggctggaaat ccgccgccag cagccggatt tcgtgattct gcgcagctgg
     8941 ggggtgatga ccccggcggc gatcaaggcc gccgccaagg tgggcttccc gcgtgacaag
     9001 atcgtcgccg tgtggtgggg cggttcggaa gaagacgtca tcccggctgg tgaggccgcc
     9061 aagggttata tcgccggcag cttcgcctcg gatggcgtgg gcttcccggt gattcaggac
     9121 atccagaaaa ccctgtacgc caacggcaag ggcaatctgt ccgaccaaag cgccattggt
     9181 acggtgaact acaaccgtgg cgtgatcggc gggatcatga tggtggaagc catccgcgtg
     9241 gcgcaaggca agttcggcaa aaaagcgctg aacggcgacg aagtgcgctg gggcctggaa
     9301 cacctcgacc tcagcccggc ccgcctgaag gcgctgggcg cggccaatat gatccccggc
     9361 gtgaaaacct cgtgcgccaa ccacgaaggc tcgggctaca cccgcttcat gcaatgggac
     9421 ggcaaggcct ggaagccggc ctccggctgg attgccccgg acaacagcgt gctcgacccg
     9481 ctggtgaaag cctcggccga gaagtatgcc aaagaaaagg gcatcacccc gcgcgattgc
     9541 agcaaggaaa agtaatccac ccggccccgg tgtctgccgg ggaggatgtc ctgtttgccg
     9601 gcggatgcac gttatccgcc ggccctatgg agccgcatca tgagtcaaac cggcattgtt
     9661 cccggccaca ccgaccagca gcccagcccg ttcttgctgt cggtcaacaa tattgaagtg
     9721 atttacaacc atgtgattct ggtgctcaag ggcgtgtcgc tggacgtgcc cgaaggcagc
     9781 atcgtcgcct tgctgggcgg caacggcgcg ggcaagagca ccacgctgaa agccatttcc
     9841 aacctgttgg ccagcgaacg cggcgatgtc accaagggct cggtggagtt tcgcggcgag
     9901 cgggtggatc agctcacccc ggacgcgctg gtcaagcgcg gcgtggtgca ggtgatggaa
     9961 ggccgccatt gttttggcca cctcaccgta gaagaaaacc tgctgaccgg cgcgtatacg
    10021 cgcaaggcca gccgggcgga aatcgcccag gacctggaga aggtctatca ctattttccg
    10081 cgcctgaaag tgcgccggca gagtcaggcc ggctacacct cgggcggcga gcagcagatg
    10141 gtggcggtgg gccgcgcgct gatggcgcgc ccgagcatga ttttgctgga cgagccgtcg
    10201 atgggcctgg cgccgcagat tgtggaagag attttcgaga ttgttaaaga cttgaatcaa
    10261 aaagagcggg tgagttttct gctggccgag cagaacacca tggtggcgct gcgctacgcc
    10321 aattttggct acatcctgga gaacggccgg gtggtgatgg atggcgcggc ggatgagttg
    10381 gccagcaatg aggatgtgaa ggagttttat ctgggcctgg gcggggatgg gcgcaagagt
    10441 ttccgcgagg tgaagcatta tcggcggcgt aagcggtggt tggcgtaagg gtttatctgt
    10501 ctgggtgatg tggttgcgct gacgcgcatg ggctggggct gaattggggc ggggcttcgc
    10561 cccgcacccg accctatttc tttgtctcgc caaagaaata ggggaaagaa aggcgacccg
    10621 ggcgtcgcgc cctacgcctg cggcttcggg tgccttgcgc ttctcgccgg ccagggccgc
    10681 cacggaatgg cgcgtcggct gcgccgccgc ttagccgtgg cggacacctc cctgtccggc
    10741 tgcgatgctc agcgcgccac acgggggtga acagcggcta agcgcaacgg gcaaggcact
    10801 aaaagtattg aatttataga ttattggggt tttggtgagc gctaaggttg tgcagtgcta
    10861 cccggcattt accccacccc cgcgtcgtcc ctgcgcaggc ggggacccag gcaaggaacc
    10921 acagcgtggc gacggtgaaa acgcgttggc cgtggtttgg tgagtgactc acccacccta
    10981 cgccatcaca agaatcaggt ttggccagca gcacggccag tatgttgaat acgcagcatc
    11041 tcaattcgag gatattccga tgccccatct ggacgctctg gaaacccggc cgcacgcgca
    11101 gcgtgtggcg gagcaatacg cccgtttgcc ggcgcagttg gcttatgccc gtgagcatag
    11161 cccggcttat gcccaggcgt tggccggcct ggatttgtcc gctgtcaccg atgcgcaggc
    11221 gctggcgggg ttgccggtga cgcgcaagtc ggccttgatt gagcagcagc aggcgtgccc
    11281 gccgtttggc gggtatgccg ccatggcggc tggggcagcc agtcgggtgt ttgcctcgcc
    11341 cggcccgatt tacgaacccc aggggccggg tgaggatttt tggcggatga gccgcgcgtt
    11401 gtacgccgcc gggtttcgca gcggcgatat tgtgcacaac acgttttctt atcacttcac
    11461 tccgggcggc ttcatgctgg attccggcgc gcgcgccttg ggctgcgcgg tgtttccggc
    11521 cggggtgggg cagaccgagc agcaggtgat ggcgctgcgc gatattggcg ccaccggcta
    11581 caccggcacg ccgtcgtttt tgaaaatcat cctggataaa gccgacgagc tgggcattgc
    11641 cctgccccgc ttgaacaagg cgctggtcac tggcgaggcc ttgcccaaca gcctgcgtca
    11701 gcagtttgag gcgcgcggca ttcaggtgcg ccagtgctac gccacggcgg atttggggct
    11761 gattgcctac gagagccagc ccggcgaagg cttggtggtg gacgagggca ttgtgctgga
    11821 gctggtcaag cctggcacgg gcgagttggt ggccgatggc gatgtcggcg aggtggtggt
    11881 caccccgctg cataaccgcg cctacccgct ggtgcgcttt gccacgggcg atttgtcggc
    11941 gtttctggac agcgctggca gttgcgggcg cagcaatcgc cgcatcaagg gctggctggg
    12001 ccgcgccgac cagaccgcca agatcaaggg catgtttgtg cacccggcgc aaatccacgc
    12061 catcggcccg cgccatccgg cgctggggcg cttgcgcctg gtggtggagc gggtgaacga
    12121 cctggaccac atgaccttgc acgccgagag cgccgacagc gacgccgcgc tgcgccaggc
    12181 ggtggccgac agcctgcgcg agctgaccaa gctcaagggc gaggtgtgcc tgtgcgcgcc
    12241 gggcagcctg gccaacgacg gcaagattat tgacgaccag cgtccgctgg cctgacccac
    12301 acagaggccg ggcgccacgt ccgcccggcc cggataacgc tggcggggcg atacgccccc
    12361 acccaattag gagacactgc atgaccgcca ccttgcctac cgttccgctg ctgattcagg
    12421 gcgaatttgt cgagtccgcc accacgcaat ggcgcgatgt ggtcaacccg gccacccagg
    12481 agccgctggc ccgcgtgccg ttcgccacgg ccgatgaaat caacgccgcc atcgccagcg
    12541 ccaaggctgc gttcaaaacc tggcgcaaga cgccgattgg cgcgcgcgcg cggatttttc
    12601 tcaagctgca acagctgatc cgcgaaaaca tggccgatct ggccgccacg ctgaccgccg
    12661 agcaaggcaa aaccctggct gacgccgagg gcgatgtgtt ccgtggcctg gaagtggtcg
    12721 agcacgccgc caatatcggc acgctgcaaa tgggcgagtt tgccgaaaac gtcgccagcg
    12781 gcgtggacac ctacaccctg ctgcaaccgc tgggcgtgtg cgccggca
//
LOCUS       DAOPYX010000044        12827 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_5407_length_12827_cov_11.263858, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000044 DAOPYX010000000
VERSION     DAOPYX010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 12827)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12827)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12827
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5407_length_12827_cov_11.263858"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(5..1966)
                     /locus_tag="PKN55_03735"
     CDS             complement(5..1966)
                     /locus_tag="PKN55_03735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012696091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HNX70386.1"
                     /translation="MSKSYDESSVRVLKGLEPVKERPGMYTRTTDPTHICQEVIDNAA
                     DEALGGFARKIAVTLHLDGSLSVEDDGRGIPVGLHPDEGVPVVELVFTRLHAGGKFNK
                     KDGGAYAFSGGLHGVGVSVTNALSTRLEVEVKRESAVWQLAFSGGEVIEPLRKLGDCP
                     PRTSGTRVRVWPDARYFESPRYSMAELERLLRAKAVLLPGVAVTLTLEKADGQHEVRS
                     WRYPDGLKSYLVELYGDDEPVAPAFAGENYIGAEHEQFSPGEGAAWALAWFEHNGGGE
                     SYVNLIPTASGGTHEAGLRTGVFEAVKSFVDHHNLLPRGVKLQAEDVWGRVRFVLSAR
                     VLDPQFQGQTKEKLNNRDALKLVASLVRDPLEIWLNAHVDHARKIAELAIRQAQSRLK
                     SVKKVEKKKGSGVAVLPGKLTDCESEDIQRNELFLVEGDSAGGSAKLARDKEYQAILP
                     LRGKVLNSWETDRDQLFSNAEIHDIAVAIGVDPHGADDSPDLSGLRYGKIAILSDADV
                     DGSHIQVLLLTLFYRHFPALIRAGHIYVAKPPLYRVDVPGQGKTRPPRKFYALDDAEL
                     DSMLERLRSEHVREGSWSIGRFKGLGEMNPEQLKDTTMHPDTRRLSRVVLPPVGTADA
                     TFTMLMGKGEAASRRAWMEEKGNLVEADV"
     gene            complement(2055..2594)
                     /locus_tag="PKN55_03740"
     CDS             complement(2055..2594)
                     /locus_tag="PKN55_03740"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012696089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HNX70387.1"
                     /translation="MLDRDGYRPNVGIILCNARNEVFWGKRVREHSWQFPQGGIKPGE
                     SPEAAMYRELMEEVGLSPHHVKILGRTRDWLRYDVPTHWIRREWRGSYRGQKQIWFLL
                     RLTGRDNDVCLRATNHPEFDAWRWNTYWAPVDAVIEFKRDVYERALGELAKFLKGLDP
                     RQGLAPPSLPEAPPAAARR"
     gene            complement(2730..3302)
                     /locus_tag="PKN55_03745"
     CDS             complement(2730..3302)
                     /locus_tag="PKN55_03745"
                     /inference="COORDINATES: protein motif:HMM:NF015823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-type transport auxiliary lipoprotein family
                     protein"
                     /protein_id="HNX70388.1"
                     /translation="MKPVIVRLLPCLAALALAACASAPLAETRYRLPEPAPLSAAQPA
                     MPALAVTVEQAAWLNGLAMHYQLDAQRPQLARYRDSLWADRPAALLAARLRQRLSAIP
                     VAAGAPWSLRLYLDRFEQVYQPDLHSQGEARWQAELRDSHGALRARHTFSASAPASQA
                     HAEAGARALGAASDALIEDTLRWAREGGVR"
     gene            complement(3299..4156)
                     /locus_tag="PKN55_03750"
     CDS             complement(3299..4156)
                     /locus_tag="PKN55_03750"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HNX70389.1"
                     /translation="MESKAFALWTGLFVIALLAALAAVVIWMSGVRQGERVEYTLLSR
                     QSVSGLNREAAVQYRGIPVGKVVELGIDPADPKQVRVRVAVDSTVRLSAQAWARLGTQ
                     GLTGLTFVELHDDGGPPWRDPAGAPLPMRPSLWQDTTSKLPAALGEARQLGERLNLLL
                     DDANRRELASLLANLNRFSAQLPALGEQSTATLASARQLADELRQLSQGLRAVLAQAG
                     PAVGQASARLNQDTLPRLDQLASQAEHTAQALDGLIDSHSRRPQQLLLGAPMDPPGPG
                     EAGFAGAAR"
     gene            complement(4146..4910)
                     /locus_tag="PKN55_03755"
     CDS             complement(4146..4910)
                     /locus_tag="PKN55_03755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001325019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNX70390.1"
                     /translation="MSAPAVRLRGVSTRFGERVIHHQLDLDVQQGEVLALVGASGCGK
                     TTLLRHMIGLTQPAAGVVEVLGVRVSPEQFLAQRSLRQRCGVLFQSGALFSALSVLEN
                     VALPLLERGGLGRADAAHIARCKLAQVGLAAEDGDKLPAQLSGGMIKRAALARALALD
                     PELLFLDEPTAGLDPLGAQAFVALIAELRALHGLTAVMVTHELERVAPLLDRVAVLAD
                     RRVVAIGPLAAVRRADHPFIQAYFAAHDPQGENHGK"
     gene            complement(4907..6022)
                     /locus_tag="PKN55_03760"
     CDS             complement(4907..6022)
                     /locus_tag="PKN55_03760"
                     /inference="COORDINATES: protein motif:HMM:NF014460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNX70391.1"
                     /translation="MNPVSAAPPTLHLSADGEVQLAGRWTFAGLRDHAELRAAVRAQS
                     PQARWDVSHAELDAVGALWLWQDWQGQLPAALRATEAQRTLWRDLAAAQAPAPTPIRR
                     DALAPLRAVGRGALALLTQAQLVCMLWGRLLLASLHLLRHPSALPARELSANIYHAGV
                     SALGITALVGLLIGVVLSYLSSQQLARYGANVLIVDLLGISILRELGPLLAAILVAGR
                     SGSAMTAQLGTMKLNQELDALAAMGLSATLRLAWPKTVALLLVLPLLAVWTSALALVG
                     GMLAAHWELGLSFGQFLDRFPRVVKLVHLWIGLGKSAAFGLLIGLIGCHFGLTAPPSS
                     EGLGRATTRAVVAAITLVIVADALFAVLFSITLRPRP"
     gene            complement(6019..6807)
                     /locus_tag="PKN55_03765"
     CDS             complement(6019..6807)
                     /locus_tag="PKN55_03765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018747779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HNX70392.1"
                     /translation="MTPTPLRLAARDALALTELPAKLSAVAALDLAAPCGAELALTPP
                     TPLPGRPARPELIPPSQLAQRGLGTREGRAALIHALAHIELNAIDLALDIVWRFAGLP
                     DAFYRDWLGVAQEEAEHFQLLNAHLNTLGHQYGDFPAHNGLWDMAERTADDLLARLAL
                     VPRTLEARGLDVSPGIQAKLRQVGDLDAVAILDVILRDEIGHVALGVRWFAEFCAARG
                     LEPVAHAEALRLAYRAPRPRGPFNLAARRQAGFADAELAALDAR"
     gene            complement(6950..9334)
                     /gene="ppsA"
                     /locus_tag="PKN55_03770"
     CDS             complement(6950..9334)
                     /gene="ppsA"
                     /locus_tag="PKN55_03770"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HNX70393.1"
                     /translation="MAENYVVWFDKLRMTDVETVGGKNASLGEMISQLASSGVRVPGG
                     FATTAQAYRDFLAHNGLADRINAMLAKLDVDDVVELARVGKEIRQLIVDTPFPAQLEA
                     EIRTAYDQMVADSGAAISVAVRSSATAEDLPDASFAGQQETFLNILGFENVMAAIKHV
                     FASLYNDRAISYRVHKGFAHDVVALSAGIQRMVRSDCGAAGVMFTIDTESGFNDVVFV
                     TASYGLGETVVQGAVNPDEFYVHKPTLKAERPAILRKNLGSKLIKMEFTDTREAGRSV
                     KTLDVAPELRTQFSINDADVEELARFALIIEKHYDRPMDIEWGKDGVDGKLYILQARP
                     ETVKSQESRVDTLRRYRLKNKSNVLVEGRAIGQKVGQGVVRLIKDASEMDRVKPGDVL
                     VTDMTDPDWEPVMKRAAAIVTNRGGRTCHAAIIARELGIPAVVGCGNATDVLKDGMQV
                     TVSCSEGDTGNIYDGLLEVEIIDLALDQMPDSPVKIMMNVGNPELAFDFCQLPNEGVG
                     LARMEFVINRQIGIHPKALLELDSLSPELQADIRSRIAGYASPVDFYVDKIAEGVATL
                     AAAFYPKKVIVRMSDFKSNEYANLVGGSLYEPHEENPMIGFRGAARYIAPEFRDCFEL
                     ECRAIRKVRDVMGLTNVEVMIPFVRTLKEAESVVQILKDFGLERGKNGLRLIMMCELP
                     TNAVLAEQFLEHFDGFSIGSNDMTQLTLGVDRDSGGPIAPTFDERDPAVKAMLHMAIN
                     ACRRLNKYVGICGQGPSDHPDFAKWLVEEGIETISLNPDTVVETWLYLAKEVQK"
     gene            9501..10325
                     /locus_tag="PKN55_03775"
     CDS             9501..10325
                     /locus_tag="PKN55_03775"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012698114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HNX70394.1"
                     /translation="MPHRRSAFFVSDRTGITAESLGHTLLTQFEATEFKRETIPFIDT
                     PDKAGEVSRRIAERAQSDHHRPLVFLSIIDPGVRKAIEVDDCALVIDLFDSFISTLEQ
                     ELGQHASRAAGKSHGITNEKTYHHRIDAVNFSLNHDDGVKPSDLAEADVILVGVSRSG
                     KTPTCLYLALQYGIRAANYPLTPEDFGKPILPKLLLPHRHKLFGLTITPDRLVQIRSE
                     RRPNSTYASLDNCRFEVKEAEAMMRQQGVTFISTTHKSIEEIASTIIHSARLTKRF"
     gene            complement(10374..12500)
                     /locus_tag="PKN55_03780"
     CDS             complement(10374..12500)
                     /locus_tag="PKN55_03780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018746352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HNX70395.1"
                     /translation="MTGARTAPFLPMSRAEMKQLGWDACDIILVTGDGYIDHPSFGMA
                     LIGRLLEAQGFRVGIISQPDWKSAEPFRALGRPRLFFGVTAGNMDSMINRYTADRKPR
                     SDDAYTPHGEAGKRPDRAVVVYAQRCREAFPGVDIMIGGIEGSLRRIAQYDYWSDKVR
                     QSVLAYAKADLLLFGNAERALLEVAHRAAHGERLAQMRDIRGTAFLTSQGWRPDDSWQ
                     EIDSTTVDAPGRVDPHPDPYAMEPVNPAPSDPSAPQPVRLISAAERVRQRKAQRAKTV
                     VRLPAYEAVAYDPVLYAHASRTLHLESNPGNARALVQAHGERDVWINPPPIPLTTPEM
                     DYVYDLHYARQPHPSYGNARFPAWDMIRFSVNIMRGCFGGCTFCSITEHEGRIIQSRS
                     EESILQEIEHIRDKTPGFTGHITDLGGPTANMYRLACKDRNIEASCRRLSCVYPGICE
                     NLNTDHSDLIQLYRKARAVPGVKRITIGSGVRYDLAVESPEYIRELATHHVSGYLKIA
                     PEHTEDGPLSKMMKPGIGSYERFKALFERFSQQAGKKQYLIPYFIAAHPGTSDEDMLN
                     LALWLKHNGFKPDQVQAFTPTPMALATTMWHTGRNPLKKLARSSEKVEVIKDAYRRKL
                     HKAFLRYHDAENWPLLREALVKMGRADLIGHGPQQLIPPESGAARPGAAKSVRAPHGK
                     PLGKRPTVAGKPQSRPPATQKKTGRR"
BASE COUNT         2230 a         4136 c         4243 g         2218 t
ORIGIN      
        1 ccgtctacac atccgcctcc accagattgc ctttttcttc catccacgcc cggcggctgg
       61 cggcttcgcc tttgcccatc agcatggtga aggtggcgtc ggccgtgcca accggcggca
      121 gcaccacgcg gctgagccgg cgggtgtcgg ggtgcatggt ggtgtctttc agttgctcgg
      181 ggttcatttc gcccaggcct ttgaagcggc cgatgctcca gctgccttcg cgcacatgtt
      241 cgctgcgcaa ccgctccagc atgctgtcca gctcggcgtc gtccagcgcg tagaatttgc
      301 gcggcgggcg ggttttgcct tggccgggca cgtccacgcg gtacagcggc ggcttggcca
      361 cgtagatgtg gccggcgcgg atcagcgccg gaaagtggcg atagaacagc gtcagcagca
      421 gcacctggat gtgcgagccg tccacgtcgg cgtcagacag gatggcgatt ttgccgtagc
      481 gcaggccaga cagatccggg ctgtcgtcgg cgccgtgcgg gtctacgccg atggcgacgg
      541 cgatgtcgtg gatttcggca ttcgagaaca attggtcgcg gtcggtttcc cagctgttga
      601 gcaccttgcc acgcagcggc aggatggcct ggtattcctt gtcgcgggcc agtttggccg
      661 acccgccggc cgaatcgcct tccaccagaa acagctcgtt gcgctggatg tcttcgcttt
      721 cgcagtcggt cagcttgccg ggcagcaccg ccacgccgga gccttttttc ttttcgactt
      781 ttttcaccga cttcagccgg ctttgcgcct ggcggatggc cagttcggcg attttgcgcg
      841 cgtggtccac atgggcgttc agccagattt ccagcgggtc gcgcaccagg ctggccacca
      901 gtttcagcgc gtcgcggttg ttgagttttt ctttggtctg gccctgaaac tgcgggtcga
      961 gcacccgcgc cgacagcaca aagcgcaccc ggccccacac gtcttcggct tgcagtttga
     1021 cgccgcgcgg cagcaggttg tggtgatcga caaagctttt caccgcctcg aacaccccgg
     1081 tgcgcaggcc ggcttcgtgg gtgccgccgc tggcggtggg gatcaggttg acatagcttt
     1141 cgccgccgcc gttgtgctca aaccaggcca gcgcccaggc ggcgccttcg ccgggggaga
     1201 attgctcgtg ctcggcgcca atgtaattct cgccggcaaa agccggggcg acaggttcgt
     1261 cgtcgccata cagttcaacc aggtagcttt tcaggccgtc cgggtagcgc cagctgcgca
     1321 cctcgtgctg gccgtcggct ttttccaggg tgagggtcac cgccacgccg ggcagcagca
     1381 ccgccttggc gcgcagcagg cgctccagct cggccatgga ataacgcggg ctttcaaaat
     1441 agcgcgcgtc cggccacacg cgcacgcggg tgccgctggt gcgcggcggg caatcgccca
     1501 gcttgcgcag gggctcgatc acctcgccgc cagaaaacgc cagctgccag accgcgcttt
     1561 cgcgtttgac ttccacttcc agccgggtgg acagcgcatt ggtcaccgac acgcccacgc
     1621 cgtgcaggcc gccagaaaac gcatacgcgc cgccgtcttt tttattgaac ttgccgccag
     1681 cgtgcaggcg ggtgaacacc agctcgacca ctggcacgcc ttcgtccggg tgcaggccaa
     1741 ccggaatccc ccggccgtcg tcctccacgc tcagcgagcc gtccaggtgc agggtgacgg
     1801 cgattttgcg ggcaaagccg cccagcgctt cgtcggcggc gttgtcgatg acttcctggc
     1861 agatgtgggt ggggtcggtg gtgcgggtgt acatgcccgg ccgttccttg accggctcca
     1921 ggcccttgag cacgcgcacg gaagattcgt cgtaggattt gctcatcggt ggcattcagt
     1981 gaagactgcg ccgccgcgtc tgggcggcgg caaaaaacaa atatcccgcc gacaggcggg
     2041 atgcggatgg ggcgtcagcg gcgcgccgca gcgggcgggg cctcgggcag ggacggcggg
     2101 gccaaaccct ggcgcgggtc cagccctttc aaaaacttgg ccagctcgcc cagcgcgcgc
     2161 tcgtaaacgt cgcgcttgaa ttcaatcacc gcgtccaccg gcgcccaata ggtgttccag
     2221 cgccaggcgt cgaactcggg gtgattggtg gcgcgcaggc acacgtcgtt gtcacggccg
     2281 gtcagccgca acaaaaacca gatttgcttc tggccgcgat agctgccgcg ccactcacgg
     2341 cgaatccagt gcgtgggcac gtcgtagcgc agccagtcgc gggtgcgccc cagaatcttg
     2401 acgtggtgcg gcgacaggcc gacttcttcc atgagttcgc ggtacatggc cgcctccggg
     2461 ctttcgcccg gcttgatgcc cccttgcgga aactgccacg agtgctcgcg cacccgctta
     2521 ccccagaaca cttcattgcg ggcgttgcag aggatgattc cgacgttggg gcggtaaccg
     2581 tcccggtcta acatgatgca acctgctcaa tatccaatgg cctgaattct ggcacatttc
     2641 tcgccaataa gacagacggg gcagggcaaa gggcgtcagc gggcggtttt ttggcggatg
     2701 agcgcaggcg cgaatccacc atcaggtctt tacctcacgc ccccctcgcg cgcccagcgc
     2761 aaggtgtctt caatcagcgc gtcgctggcg gcgcccagcg cgcgcgcgcc ggcttcggcg
     2821 tgcgcctggc tggccggggc gctggcgctg aaggtgtggc gggcgcgcaa ggcgccgtga
     2881 ctgtcacgca gttccgcctg ccagcgcgct tcgccctggc tgtgcagatc gggctggtaa
     2941 acctgttcga agcggtccag atacaggcgc agcgaccagg gcgcgccggc ggccaccggg
     3001 atggcggaca ggcgttggcg cagacgggcg gccagcagcg cggccgggcg gtcggcccac
     3061 aggctgtcgc ggtagcgcgc cagttgcggg cgctgggcgt ccagttggta gtgcatggcc
     3121 aggccgttca gccaggcggc ttgctcgacg gtgaccgcca gcgccggcat ggccggctgg
     3181 gcggcgctca gcggggcggg ttcgggcagg cggtagcggg tttccgccag cggggcgctg
     3241 gcgcaggcgg ccagcgccag cgcggccagg cagggcaaca ggcggacaat cacgggcttc
     3301 atcgtgcggc tccggcaaag ccggcctctc cggggccggg gggatccatc ggcgcgccca
     3361 gcagcaattg ctgcgggcga cggctgtggc tgtcgatcaa gccatccagc gcctgggcgg
     3421 tgtgctcggc ctggctggcc agttgatcca gccggggcaa ggtgtcctgg ttcaggcggg
     3481 cgctggcctg accgacggcg gggccggcct gggccagcac ggcgcgcagg ccttgcgaca
     3541 gctggcgcaa ttcgtcggcc agttggcggg cgctggccag ggtggcggtg gattgctcgc
     3601 ccagcgccgg cagctgcgcc gaaaagcggt tcagattggc cagcaggctg gccagctcgc
     3661 ggcggttggc gtcgtccagc aagagattca agcgctcacc cagctggcgc gcctcgccca
     3721 atgccgccgg cagcttgctg gtggtgtctt gccacagcga tggccgcatc ggcagcggcg
     3781 cgccggccgg gtcgcgccag ggcgggccgc cgtcgtcgtg cagctcgaca aaggtcagcc
     3841 cggtcagccc ttgcgtgccc agccgcgccc acgcctgggc gctgagccgc accgtgctgt
     3901 ccaccgccac gcgcacgcgc acttgcttgg ggtcggccgg gtcgatgccc agttcgacca
     3961 ccttgcccac cgggatgccg cgatactgca ccgccgcttc gcggttcaag ccgctgaccg
     4021 actggcgcga gagcagggtg tattccaccc gctcgccctg gcgcacgccg ctcatccaga
     4081 tcaccaccgc cgccagcgcg gccagcaggg cgatgacaaa caggccggtc cacagggcaa
     4141 aggccttact ttccatggtt ctccccttgc gggtcatgcg cggcgaaata cgcctgaata
     4201 aaagggtgat cggcgcggcg cacggccgcc agcgggccga tggccaccac gcggcggtca
     4261 gccagcaccg ccacgcggtc caagagcggc gccacgcgct ccagctcgtg ggtgaccatc
     4321 accgccgtca gcccgtgcag cgcccgcagc tcggcaatca gcgccacaaa ggcctgcgcg
     4381 ccaagcgggt ccagcccggc ggtgggttcg tccaaaaata ataattccgg gtccagcgcc
     4441 agggcgcggg ccagggcggc gcgcttgatc atgccgccgg acaattgcgc cggcagtttg
     4501 tcgccatcct ccgccgccag gcccacctgc gccagcttgc agcgggcgat gtgggcggcg
     4561 tcggcgcggc caaggccgcc acgctccaac aagggcaggg cgacgttctc cagcacgctc
     4621 agcgccgaaa acagcgcgcc actctgaaac agcacgccac agcgctggcg cagactgcgc
     4681 tgggcgagaa actgctcagg gctcacccgc acgcccagca cctccaccac gccggccgct
     4741 ggctgggtga ggccgatcat atggcgcagc aaggtggttt tgccgcagcc agaggcgccg
     4801 accagcgcca gcacttcgcc ttgctgcacg tccagatcaa gctgatggtg aatcacccgt
     4861 tcgccaaaac gggtgctcac cccgcgcagc cgcacggccg gcgcgctcat ggccgtggcc
     4921 tcagggtgat ggaaaacagc acggcaaaca gcgcgtcggc gacaatcacc agggtgatgg
     4981 ccgccaccac ggcgcgggtg gtggcgcggc ccaggccttc gctggatggc ggcgcggtca
     5041 ggccaaaatg gcagccaatc aggccaatca gcaagccaaa cgccgccgat ttgcccaggc
     5101 caatccacaa atgcaccagc ttgaccacgc gcggaaaacg atcgagaaac tgaccaaacg
     5161 acaagcccag ctcccaatgc gccgccagca tgccgccgac cagcgccagc gcgctggtcc
     5221 acaccgccag caagggcagc accaacagca gggcaacggt ttttggccag gccaggcgca
     5281 aggtggcgga caagcccatg gcggccagcg cgtcgagctc ttgattcagc ttcatcgtgc
     5341 ccagttgcgc ggtcatcgcc gagcccgagc gcccggccac cagaattgcc gccagcagcg
     5401 ggcccagctc gcgcagaatg ctgatgccga gcaaatcgac aatcagcaca ttggcgccgt
     5461 agcgcgccag ctgctgcgag gacaaataag acagcaccac gccaatcaag aggccgacca
     5521 gcgcggtgat gcccagcgca gacaccccgg cgtggtaaat attggccgac agctcgcgcg
     5581 ccggcagcgc cgatggatgg cgcagcaggt gcaggctggc cagcagcagc cgcccccaca
     5641 gcatgcacac cagttgcgcc tgcgtcagca aggccaatgc gccgcgcccc acggcgcgca
     5701 gcggcgccag cgcatcgcgg cggatgggcg tcggcgcagg cgcttgggcg gcggccaggt
     5761 cgcgccacag cgtgcgctgg gcctcggtgg cgcgcagcgc ggcgggcagc tggccttgcc
     5821 agtcctgcca cagccacagc gcgcccaccg cgtccagctc ggcatggctg acgtcccagc
     5881 gcgcctgggg cgactgcgcg cgcacggcgg cacgcagctc ggcgtggtcg cgcaggccgg
     5941 caaatgtcca gcggccagcc agttgcacct caccgtcggc gctcaggtgc agcgttggcg
     6001 gagcggcgga gacggggttc accgcgcgtc cagcgccgcc agttcggcgt cggcaaaacc
     6061 agcctggcgg cgggcagcca ggttgaacgg cccgcgcggg cgcggcgcgc ggtaggccag
     6121 gcgcagcgct tcggcatggg ccacaggctc caggccgcgc gcggcgcaaa attcggcaaa
     6181 ccagcgcacg cccagcgcca catggccgat ttcatcgcgc agaatcacat ccagaatcgc
     6241 caccgcgtcc aggtcgccca cctggcgcag cttggcctga atgccgggcg agacgtccag
     6301 cccgcgcgct tccagcgtgc gcggcaccag cgccagccgc gccagcaggt catcggcggt
     6361 gcgctcggcc atgtcccaca ggccgttgtg agccggaaaa tccccgtact ggtggcccag
     6421 ggtgttcagg tgggcgttca gcagctgaaa atgctcggct tcttcctgcg ccacgcccag
     6481 ccagtcgcgg taaaaggcgt caggcaggcc ggcaaagcgc cagacgatgt ccagcgccag
     6541 gtcaatggcg ttgagttcga tatgcgccag cgcgtggatc agcgcggcgc ggccttcccg
     6601 cgtgcccagg ccgcgctgag ccagttggct gggcgggatc agctccgggc gggccgggcg
     6661 gccgggcagc ggcgtcggcg gggtgagggc aagctcggcg ccgcagggcg cggcaaggtc
     6721 cagcgcggcg acggcgctga gtttggcggg cagttcggtc agcgccagcg cgtcacgggc
     6781 ggccaggcga agaggggtgg gcgtcatggc aaatcggtca acaggggtca agttggcgac
     6841 gcggccaggc gcgtgcgctg gccagcagac cgccaccata gcaaaaagtg cgcggccata
     6901 cgcaaaaaac ccccatcgct gggggttttt tgttgcgctc ggcaagtgct tacttctgca
     6961 cttccttggc caggtacaac caggtttcca ccacggtgtc cgggttcagc gagatggtct
     7021 cgatgccctc ttccaccagc cacttggcga agtccgggtg atcggacggg ccttggccgc
     7081 agatgccgac atacttgttc aggcggcggc aggcgttgat ggccatgtgc agcatggctt
     7141 tcaccgccgg gtcgcgctcg tcgaaggtcg gggcaatcgg gccgccggag tcgcggtcca
     7201 cgcccagggt cagctgggtc atgtcgttgg agccgatgga gaagccatcg aagtgctcca
     7261 ggaactgttc ggccagcacg gcgttggtcg gcagttcgca catcatgatc aggcgcaggc
     7321 cgttcttgcc gcgctccagg ccgaagtcct tgaggatctg caccaccgat tcggcttcct
     7381 tgagggtgcg cacgaacggg atcatcactt cgacattggt caggcccatc acgtcgcgca
     7441 ccttgcggat ggcgcggcat tcgagctcga agcagtcgcg gaattccggg gcgatgtagc
     7501 gggcggcgcc acggaagccg atcatcgggt tttcttcatg cggctcgtac agcgaaccgc
     7561 cgaccaggtt ggcgtactcg ttcgacttga agtcggacat gcgcacgatg acctttttcg
     7621 ggtagaacgc cgcagccagg gtggccacgc cttcggcgat cttgtccacg tagaagtcca
     7681 ccgggctggc gtagccggcg atgcggctgc ggatgtcggc ttgcagttcc ggcgacaggc
     7741 tgtccagctc cagcagggct ttcgggtgga tgccgatctg acggttgatg acgaactcca
     7801 tgcgcgccag gcccacgcct tcgttgggca gctggcagaa gtcgaaggcc agctcagggt
     7861 tgccgacgtt catcatgatc ttgaccgggg aatccggcat ttgatccagc gccaggtcga
     7921 tgatttcgac ttccagcagg ccgtcataga tgttgccggt gtcgccttcc gagcacgaca
     7981 cggtgacttg catgccatcc ttcagcacgt cggtggcgtt gccgcagccg accacagccg
     8041 ggatgcccag ttcgcgggcg atgatggcgg cgtggcaggt gcgaccgcca cggttggtga
     8101 cgatggcggc ggcgcgcttc atcaccggct cccagtcggg gtcggtcatg tcggtgacca
     8161 gcacatcgcc cggcttgacg cgatccattt cggaggcgtc cttgatcagg cgcaccacgc
     8221 cctggcccac tttctggcca atggcgcgac cttccaccag cacattgctc ttgttcttca
     8281 ggcggtagcg gcgcagggtg tccacgcggg attcttgcga cttcacggtt tccgggcgag
     8341 cttgcaggat gtacagcttg ccgtccacgc cgtccttgcc ccactcgatg tccatcggac
     8401 ggtcgtagtg tttttcgatg atcagggcaa agcgggcgag ttcttcgaca tcagcgtcgt
     8461 tgatggagaa ctgggtgcgc agttccgggg cgacatccag ggttttcacc gaacggccgg
     8521 cttcgcgggt gtcggtgaat tccatcttga tcagcttgga gccgaggttc ttgcgcagga
     8581 tggccgggcg ctcagccttg agggtcggct tgtgcacata gaattcatcc gggttcacgg
     8641 cgccttgcac cacggtttcg cccaggccgt acgaggcggt gacgaacacc acgtcgttaa
     8701 agccggattc ggtgtcgatg gtgaacatca caccggcggc gccacagtcg gagcgcacca
     8761 tgcgctggat gccggcggac agggccacca catcgtgggc aaagcccttg tgcacgcggt
     8821 aggaaatggc gcggtcgttg tacagcgagg cgaacacatg cttgatggcc gccatcacgt
     8881 tctcgaagcc gagaatgttc aggaaggttt cctgctgacc ggcgaacgag gcgtcgggca
     8941 agtcttctgc ggtggcggaa gaacgcacgg ccaccgagat ggcagcgccg gagtcggcga
     9001 ccatctggtc gtaagcggtg cggatttctg cttccagctg cgccgggaac ggagtgtcca
     9061 cgatcagttg gcggatttcc ttgccaacgc gagcgagctc gacgacatcg tccacgtcaa
     9121 gcttggcgag catcgcgttg atgcggtcgg ccaggccgtt gtgagcgaga aaatcgcggt
     9181 aagcctgcgc agtggtggcg aaaccgccag gcacgcgcac gccggagctg gcgagctggc
     9241 taatcatttc gcccagcgac gcatttttgc cgccgacggt ttccacgtcg gtcatgcgca
     9301 gcttgtcgaa ccagacaacg taattctctg ccatgtaacg aacttcctgt gctagatggg
     9361 ggctatcgaa aaccggattc tatccgaaag cgtaaacttt gcgtaacctt aatgcactgc
     9421 acaaaagccg gatttgccct gcgaaagtgc atgttcccac gggtataatc aagcgcaatg
     9481 cccatttagc cggagttcac atgccacatc gccgctcagc cttctttgtt tccgaccgca
     9541 ccggcatcac cgccgaatcc ttgggccaca cgctgctgac ccagttcgag gccacggaat
     9601 tcaagcggga aaccattccg ttcatcgaca cgccagacaa ggccggcgag gtcagccgac
     9661 gcattgccga gcgcgcgcag agcgaccatc accgcccgct ggtgtttctg tccattatcg
     9721 acccaggcgt gcgcaaggcc attgaggtgg acgattgcgc gctggtgatt gatctgtttg
     9781 attcgttcat cagcacgctg gaacaagaac tcggccagca cgcatcacgg gcagcgggca
     9841 aatcccacgg catcaccaac gagaaaacct atcatcatcg aatcgacgcg gtgaatttct
     9901 cgctcaatca tgacgacggc gtgaaaccga gtgatctggc tgaagccgat gtcattctgg
     9961 tgggggtgtc gcgctcgggc aaaaccccga cctgcctgta tctggcgctg caatacggca
    10021 ttcgtgccgc caattatccg ctgacgccgg aagattttgg caaaccgatt ctgcccaaat
    10081 tgctgctgcc gcatcgtcac aaattgtttg gcctgaccat taccccggat cgcctggtgc
    10141 aaatccgctc ggaacgccgg ccaaacagca cttacgcctc gctggacaat tgccgctttg
    10201 aggtgaaaga agccgaggcg atgatgcgcc agcagggggt gacgtttatc agcaccacgc
    10261 acaaatccat cgaagaaatc gccagcacca tcatccacag cgcccggctg accaagcgtt
    10321 tttaagcgcg gttcagccaa gccggcagac gcgctgacgc gcgcctgacc ggcttaacgg
    10381 cggccggttt ttttctgcgt tgctggcggc cgtgactgcg gctttcctgc aacagtcggg
    10441 cgtttgccca gcggtttgcc gtgcggcgcg cggaccgatt tggccgcgcc agggcgcgcg
    10501 gcgccgcttt ctggcggaat cagctgctgc gggccatggc caatcaggtc ggcgcggccc
    10561 atcttcacca gcgcttcgcg cagcaagggc cagttctcgg cgtcgtgata gcgcaaaaac
    10621 gccttgtgca gtttgcgccg gtaggcatcc ttgatcactt ccactttttc agagctgcgc
    10681 gccagttttt tcagcggatt gcgcccggtg tgccacatgg tggtggccaa ggccatcggc
    10741 gtgggggtga acgcctgcac ctggtcgggt ttgaagccgt tgtgcttcaa ccacagcgcc
    10801 aggttgagca tgtcttcgtc gctggtgccg gggtgggcgg cgataaagta gggaatcaga
    10861 tactgcttct tgccggcctg ctggctgaaa cgctcaaaca gcgccttgaa gcgctcgtaa
    10921 ctgccaatgc ccggcttcat catcttggac aaaggcccgt cttcggtgtg ctccggggcg
    10981 attttcagat agccgctgac gtggtgggtg gccagctcgc ggatgtattc cggcgactcc
    11041 accgccaggt cgtagcgcac gcccgagcca atggtgatgc gcttcacccc cggcacggcg
    11101 cgcgccttgc gatacagctg gatcaaatcg ctgtggtcgg tgttgaggtt ttcgcaaatg
    11161 cccggataca cgcaggacaa gcggcgacag gaggcttcaa tattgcggtc tttgcacgcc
    11221 agccggtaca tattggcggt cggcccgccc aggtcggtga tatggccggt aaaccccggt
    11281 gttttgtcgc gaatatgctc gatttcctgc aaaatcgatt cttctgaacg gctttgaata
    11341 atgcggcctt cgtgctcggt gatcgagcag aaggtacagc cgccaaagca gccgcgcatg
    11401 atgttcaccg aaaaacggat catgtcccag gccggaaaac gggcattgcc atagctgggg
    11461 tgcggctggc gggcgtagtg cagatcgtaa acgtagtcca tctccggcgt ggtcagcgga
    11521 atcggcggcg ggttgatcca cacatcgcgc tcgccatgcg cctgcaccag cgcgcgggca
    11581 ttgccggggt tggattccag gtgcagggtg cggctggcgt gggcgtacag cactggatcg
    11641 taggccacag cttcatacgc cggcaaacgc accacggttt tggcgcgctg cgccttgcgc
    11701 tggcgcacgc gctcggcggc ggaaatcaat cgcaccggct gcggcgcgct ggggtcgctg
    11761 ggcgccgggt tgaccggctc catggcgtaa gggtcgggat gcgggtccac ccggcctggc
    11821 gcatccaccg tggtcgagtc gatttcctgc cagctgtcgt ctggccgcca gccctggctg
    11881 gtcaaaaacg ccgtgccgcg aatatcacgc atctgcgcca ggcgctcgcc gtgcgcggcg
    11941 cggtgcgcca cttccagcaa ggcgcgctcg gcgttgccaa acagcagcaa atccgccttg
    12001 gcgtaagcca gcaccgattg gcgcactttg tcgctccagt aatcgtactg ggcaatccgc
    12061 cgcaagctgc cctcaatgcc accaatcatg atgtctacac ccggaaacgc ctcgcggcag
    12121 cgctgcgcat acaccaccac ggcccgatcc ggccgcttgc cggcctcgcc atgcggggtg
    12181 taggcgtcgt cagagcgcgg tttgcggtct gcggtgtagc ggttgatcat cgaatccata
    12241 ttgccggcgg tgacgccaaa aaacagccgg ggccgaccca gcgcgcgaaa cggctcggcg
    12301 cttttccagt cgggctggct gatgatgccc acccgaaaac cttgtgcttc cagcaggcgg
    12361 ccaatcagcg ccatgccaaa gctgggatgg tcgatatagc cgtcgccggt caccagaatg
    12421 atgtcgcagg cgtcccagcc cagctgcttc atttcagcgc ggctcatcgg caaaaatggc
    12481 gccgtgcgcg cgccggtcat cggctgacgc ggcagggaaa taatcgaggg cggggcggcg
    12541 gaatcggtcg aaaaagtcat gggcaggcct gggcagtgcg cggtaagaca agctggacat
    12601 tgtgccagac aatccgccac accggcaaaa aaggctttga attcatctgg atagccgtat
    12661 tgtcagcatg ggtttgccat gcatggaggc taaacgatgc aaagagaggc gtgattggga
    12721 taaacccgcg cgaagcgcgg ctcacaaaac acagcaaatt agtgagccga gctaaaaata
    12781 caaaaccccc tgtagcgtga gctacagggg gctgtaatag gagcctg
//
LOCUS       DAOPYX010000045        12532 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_5619_length_12532_cov_12.289252, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000045 DAOPYX010000000
VERSION     DAOPYX010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 12532)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12532)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12532
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5619_length_12532_cov_12.289252"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(2..418)
                     /locus_tag="PKN55_03785"
     CDS             complement(2..418)
                     /locus_tag="PKN55_03785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX70396.1"
                     /translation="MSLQPRLLLLHKHRTSGRVRYLRLPDGMLAFAPLPALAMLRDPG
                     YAPKLYFHPATVTREAEAWLALPAGSIELDAEFHCWVDTPEGDVPVLLGAFTAIDPPF
                     DAAERCAGRFIQLTEARQCREVELEILRLAYEHVLG"
     gene            complement(422..772)
                     /locus_tag="PKN55_03790"
     CDS             complement(422..772)
                     /locus_tag="PKN55_03790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX70397.1"
                     /translation="MPATLDPAVGESLCAALREQAARLGVSLGDEPIWAQASLSEAAD
                     PYTQAVSVLATWRGGARYGQATFFPDGRVFAEYQVLAPHPTRPGCFIDAVQVWGAPAA
                     WRGEAVLIELPAED"
     gene            complement(772..1050)
                     /gene="fdxB"
                     /locus_tag="PKN55_03795"
     CDS             complement(772..1050)
                     /gene="fdxB"
                     /locus_tag="PKN55_03795"
                     /inference="COORDINATES: protein motif:HMM:TIGR02936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin III, nif-specific"
                     /protein_id="HNX70398.1"
                     /translation="MPQVTGLTRGGSEWIPQFVTDLNQEKCIGCGRCYKVCPRDVLDL
                     VERELEDDDEDDDNMMVMSIANANDCIGCGSCGRVCPKDCYSYEPLAA"
     gene            complement(1056..1265)
                     /locus_tag="PKN55_03800"
     CDS             complement(1056..1265)
                     /locus_tag="PKN55_03800"
                     /inference="COORDINATES: protein motif:HMM:NF016938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCE_0567 family metalloprotein"
                     /protein_id="HNX70399.1"
                     /translation="MSEDEIQALDKEVKKLKRIASEWASQLHDLVEDRLPAAYHEIPT
                     ISQSTYDACKAWADATARLKAAQKG"
     gene            complement(1282..1752)
                     /locus_tag="PKN55_03805"
     CDS             complement(1282..1752)
                     /locus_tag="PKN55_03805"
                     /inference="COORDINATES: protein motif:HMM:TIGR02935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifX-associated nitrogen fixation protein"
                     /protein_id="HNX70400.1"
                     /translation="MSTAPVVQESDPILGTDFIREMVRQMRALDTYGVYNGQSPEKIL
                     DPFVLTRARRQEIPLVGDPDEETLARVKALYNAIAVLIEKECRLLAVPLIHLTHEGFG
                     RALITVGKLVVVDKTLRDVHRFGFDSLSKMKDEADKLLAVALERIGKYSDVAGL"
     gene            complement(1774..2214)
                     /locus_tag="PKN55_03810"
     CDS             complement(1774..2214)
                     /locus_tag="PKN55_03810"
                     /inference="COORDINATES: protein motif:HMM:NF016160.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR reducing system RseC family protein"
                     /protein_id="HNX70401.1"
                     /translation="MIEHLGIVEAVQDGKTTISVATGGCRSCHKESECGIGQVAGGRA
                     VTRLTLPAPSGLQVGESVRVRISEDSLRRSAFMGYLLPPVMMLLSAALGHFAIGGDVA
                     VAVGALTGFLFALLLARLAPARLPEVSAATPTHEATPQPNVLSH"
     gene            complement(2211..2639)
                     /locus_tag="PKN55_03815"
     CDS             complement(2211..2639)
                     /locus_tag="PKN55_03815"
                     /inference="COORDINATES: protein motif:HMM:NF014620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HNX70402.1"
                     /translation="MLKIAFASNDRTRVNQHFGAAEGFVLYEVSPTRTSMVGVAEFAP
                     EAMDGNENKLGAKVDFLEGCAAVFVMAIGASAIKQLVARGIRPIRITESDKIEDLLYD
                     IAQGMKHGGVAWIDQAMAEHARPARSADRFAAMQEEGWEG"
     gene            complement(2656..4047)
                     /gene="nifN"
                     /locus_tag="PKN55_03820"
     CDS             complement(2656..4047)
                     /gene="nifN"
                     /locus_tag="PKN55_03820"
                     /inference="COORDINATES: protein motif:HMM:TIGR01285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005488 - binding [Evidence IEA];
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogenase iron-molybdenum cofactor
                     biosynthesis protein NifN"
                     /protein_id="HNX70403.1"
                     /translation="MAEIVLSKKALAVNPLKVSQPLGASLVFLGLNKSLPLMHGSQGC
                     TAFGKVFFVRHFREPIPLQTTAMDQVSTIMGADENVIEALRTLCDKQKPEIIGLVTTG
                     LSETQGTDIHRCVREFRAAYPQYADTSVVAVNTPDTLGCLETGYALAVEALLDTLAPD
                     TRHAGRRPKQVTVLAGGHLTPGDLETLRDWIEAFGLRPVLVPDIGDSLDGHLVEAEYS
                     PLTLGGTPRAEIASLGESVATLVIGESLYRAASKLEARTGVPTYRFAGLMGLAACDAF
                     TLTLSQLSGKPVPPRLERQRAQLQDAMVDSHFMLGMARVAVAGDPDLLAMHCEFLASM
                     GAEIIAAVSPVKTDGLARLPVAKVIVGDLEDLELAARGQRVQLVVSNSHAAGTAQRLG
                     APLMRAGFPQYDWVGGFARAWVGYHGTRQALFDLANLMLSQHHDIDPYRSRYWAAERD
                     GAPLALAEAGMVH"
     gene            complement(4281..5684)
                     /gene="nifE"
                     /locus_tag="PKN55_03825"
     CDS             complement(4281..5684)
                     /gene="nifE"
                     /locus_tag="PKN55_03825"
                     /inference="COORDINATES: protein motif:HMM:TIGR01283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005488 - binding [Evidence IEA];
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogenase iron-molybdenum cofactor
                     biosynthesis protein NifE"
                     /protein_id="HNX70404.1"
                     /translation="MKASDIQALLNEPACSHNHKEKSGCARPKPGATAGGCSFDGAQI
                     ALLPIADVAHIVHGPIACAGASWDNRGTRSSGPTLYRIGMTTDLSETDVVMGRGEKRL
                     LHAIRQAVESYRPAAVFVYATCVTALIGDDMEAVCKAATDFWGVPVIPVDAAGFYGTK
                     NLGNRLAGEAMFKYVIGTREPQPAAPRPDGLPTYDVSLIGEYNIAGEFWHVAPLFDEL
                     GLRILATLSGDGRFAEVQTMHRAQANMVVCAKALLNVARKLQDNFGTPFFEGSFYGVA
                     DTSAALREFARLIGDDDLSHRTEMVIAREEALANAALAPWREKLAGKRALLYTGGVKS
                     WSIVSALQDLGMTVVATGTKKSTEEDKARIRELMGDDVRMIDDGSPKGLLSAYHDLKA
                     DILIAGGRNLYTALKARIPFLDINQEREFGYAGYTGMVELARQLHLSISSPVWPAVRQ
                     PAPWADSRRALGELLSA"
     gene            complement(6048..7019)
                     /locus_tag="PKN55_03830"
     CDS             complement(6048..7019)
                     /locus_tag="PKN55_03830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX70405.1"
                     /translation="MKDTAAWSEYCKSLAPALIHACTMAPVSTGPSALVRELANTAPD
                     WPFRHAACRGGWHRLGGLIDAKGKRLTDNLESWAETELATRQGDLGWLAEDYAEQGLL
                     ATRLVGRTHYLVAQAGDGPADFLQLEIEELQEIASHTLFADGEPATLDELLDPQLPAH
                     SGRPLGLPYYVFRRVSHAGAFVARMRAKHPEPGPIHRMLEDWAACSAGQASDFCNHWV
                     LAMSEHQDRFLQSVFRAKPMSTLEGHPPAFHGEQGQTNGALRAALTAYDRACGYPMAW
                     YFHMLVTHAVPHWVAQTIIEDAQAGFEYLPERDLAVVRDWLHRPYAL"
     gene            complement(7337..7603)
                     /locus_tag="PKN55_03835"
     CDS             complement(7337..7603)
                     /locus_tag="PKN55_03835"
                     /inference="COORDINATES: protein motif:HMM:NF040002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6129 family protein"
                     /protein_id="HNX70406.1"
                     /translation="MIATDTLDALTAEVAVNAAVAHLRGCFPGLHFTECSEDDVPVRA
                     TPVRETTDHLIYLVSGASGHCLSLTHSADAATGVLVASRVADDD"
     gene            complement(7600..8328)
                     /locus_tag="PKN55_03840"
     CDS             complement(7600..8328)
                     /locus_tag="PKN55_03840"
                     /inference="COORDINATES: protein motif:HMM:NF014620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dinitrogenase iron-molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HNX70407.1"
                     /translation="MSNTPIASREAALRIALAARTLNNLNVRALVEALADKCGLPITE
                     DRLAQLTVEDLRALLAGDYADQSCHVGVDADTLKAAIRLLWGEGVAGSDFPPVQAYAD
                     GDMPGSLRVAIASNHNELLDGHFGSCERFLIYQVSPSEIRLVGVRSTLSTADAEDKNA
                     ARAELINDCQILGVQSIGGPAAAKVVRAGVHPVKRPIGGEARDMLAELQGRLLAPPPW
                     LAQIMGVPAHSLAKFEADMEEIEP"
     gene            complement(8365..8556)
                     /locus_tag="PKN55_03845"
     CDS             complement(8365..8556)
                     /locus_tag="PKN55_03845"
                     /inference="COORDINATES: protein motif:HMM:NF012267.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNX70408.1"
                     /translation="MAMTIDSDMCTACGDCKPVCPTKSISSGKVFYKIDAATCTECDG
                     HNDTPLCVDVCPSGCIGPA"
     gene            complement(8594..8821)
                     /gene="nifT"
                     /locus_tag="PKN55_03850"
     CDS             complement(8594..8821)
                     /gene="nifT"
                     /locus_tag="PKN55_03850"
                     /inference="COORDINATES: protein motif:HMM:TIGR02934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative nitrogen fixation protein NifT"
                     /protein_id="HNX70409.1"
                     /translation="MANIMIRQAENGGLSFYLPKRDLEASIESIEFDTPEKWGGELKL
                     DNGGSYYIDPIAKPPRLPISLRAKRLGAAED"
     gene            complement(8909..10480)
                     /gene="nifK"
                     /locus_tag="PKN55_03855"
     CDS             complement(8909..10480)
                     /gene="nifK"
                     /locus_tag="PKN55_03855"
                     /EC_number="1.18.6.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016612 - molybdenum-iron nitrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0016163 - nitrogenase activity [Evidence
                     IEA];
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogenase molybdenum-iron protein subunit
                     beta"
                     /protein_id="HNX70410.1"
                     /translation="MMQTVDKIKPCYPLFGDDDYKASLKAKRDLFEEGHGADKVYETF
                     LWTTTKEYQDLNFQREALTVDPAKACQPLGAVLCSLGFEKTLPYVHGSQGCVAYFRSY
                     FNRHFKEPIACVSDSMTEDAAVFGGQKNMFDGLENAKALYKPTMIAVSTTCMAEVIGD
                     DLNAFINNAKKAGHVEQDYPVPFAHTPSFVGSHVTGWDNMMEGILRYFTLNKMEGKVV
                     GSSGKVNVVPGFETYLGNYRVIKRMLGEMDVDYTMLSDPTEVLDTPADGEYRMYAGGT
                     TIAEVEAAPNAATTLLLQPWHLEKTKKFVETTWNHDVPKLNIPMGVDWTDELLMKVSE
                     VTGKPIPDSLAKERGRCMDLISDSHAWLHGKKFAVYGDPDFVLGMVKILLECGAEPTH
                     ILAHNGNKRWAKGVQKLLDSSPFGVNGKVYVGNDLWHLRSLCFTDKPDFLIGNSYGKY
                     IQRDTKHKGDEFEVPLIRLGFPIFDRHHLHRMTTMGYEGAMYVVTTLVNAVLEQLDRE
                     TLGMGTTDYNFDLVR"
     gene            complement(10540..12012)
                     /gene="nifD"
                     /locus_tag="PKN55_03860"
     CDS             complement(10540..12012)
                     /gene="nifD"
                     /locus_tag="PKN55_03860"
                     /EC_number="1.18.6.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016612 - molybdenum-iron nitrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0016163 - nitrogenase activity [Evidence
                     IEA];
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogenase molybdenum-iron protein alpha chain"
                     /protein_id="HNX70411.1"
                     /translation="MSMTREQTEALISEVLEVYPEKAKKDRKKHLAANDTSLEQSKKC
                     ITSNRKSLPGVMTIRGCAYAGSKGVVWGPIKDMIHISHGPVGCGQYSRAGRRNYYVGT
                     TGVNSFGTMNFTSDFQEKDIVFGGDKKLTKLIEEVEQLFPLHNGISVQSECPIGLIGD
                     DIEAVSKKSAVVINKPIIPVRCEGFRGVSQSLGHHIANDAIRDWVLDKRDGQAYESTP
                     YDVAIIGDYNIGGDAWATRVILEEMGLRVVAQWSGDGTLAEMMNTPKVKLNLLHCYRS
                     MNYISRHMEEKYGIPYMEYNFFGPTKIIESIRKIAAFFDDSVKEKSEAVIARYQPMMD
                     EIVAKYKPRLQGKKVMLYVGGLRPRHVIGAYEDLGMEVIGTGYEFAHNDDYDRTLKEM
                     GDATLLYDDVTGFELEEFVKKIKPDLVGSGIKEKYIFQKMGIPLRQMHSWDYSGPYHG
                     YDGFAIFARDMDIALSSPVWSKLTPPWKTAPASAEEKKAA"
     gene            complement(12154..>12532)
                     /locus_tag="PKN55_03865"
     CDS             complement(12154..>12532)
                     /locus_tag="PKN55_03865"
                     /inference="COORDINATES: protein motif:HMM:NF012369.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX70412.1"
                     /translation="IVKYANSGGVRLAGLICNSRNTDREDELIMALASRLGTTMIHFV
                     PRDNAVQHAEIRRMTMVEYDPKHKQADEYRTLANKIVNNTNFIIPTPIEMEELEELLM
                     EFGIMEAEDESIVGKTAAELAAG"
BASE COUNT         2227 a         3874 c         4031 g         2400 t
ORIGIN      
        1 ctcaccccaa cacatgctca tacgccaggc gcagaatctc cagctccacc tcgcggcact
       61 gccgcgcctc agtaagctgg ataaaccgcc cggcgcagcg ctcggcggcg tcaaacggcg
      121 ggtcaatcgc ggtaaacgcc cccagcaaca ccggcacatc gccttctggc gtatccaccc
      181 agcaatgaaa ctcggcgtcc agctcgatgc tgccggcggg cagggccagc caggcctcgg
      241 cttcgcgcgt caccgtggcc ggatgaaaat acaatttggg cgcatagccc ggatcgcgca
      301 gcatggccag cgcaggcagc ggcgcaaagg ccagcatgcc gtcgggcagg cgcagatagc
      361 gcacgcggcc gctggtgcga tgcttgtgca acagcaacag gcgcggttgc aaggacatgg
      421 ctcaatcctc ggcgggcagc tcgatcaaca cggcttcgcc gcgccacgcg gcgggcgcgc
      481 cccaaacctg caccgcgtca ataaagcagc cgggccgcgt ggggtgcggc gccagcactt
      541 ggtattcggc aaacacccgg ccgtcgggaa aaaatgtcgc ttggccatag cgcgcgccgc
      601 cccgccaggt ggccagcacg ctgaccgcct gggtgtaagg gtcggccgct tcgctcaggc
      661 tggcctgggc ccagatgggc tcgtcgccca gcgagacgcc aaggcgcgcg gcctgctcac
      721 gcagcgccgc gcacagtgat tcgcccacgg cgggatccag cgtggccggc atcaggcggc
      781 cagcggctcg tagctgtagc agtccttggg gcagacgcgg ccacaggagc cacagccaat
      841 gcagtcattg gcgttggcga tcgacatcac catcatgttg tcgtcgtctt cgtcgtcgtc
      901 ctccagctcg cgctcgacca ggtcgagcac gtcgcgcgga cagaccttat agcagcggcc
      961 gcagccgatg catttttctt ggttcaggtc ggtgacaaac tgcggaatcc attcggatcc
     1021 gccacgggtc aggccggtga cttgcggcat gggaatcacc ctttctgtgc tgctttcagc
     1081 cgggcggtgg cgtccgccca ggccttgcag gcatcgtaag tggattgaga aatggtcggg
     1141 atctcgtggt aggccgccgg caggcggtct tccaccagat cgtgcaattg cgacgcccac
     1201 tcggaggcga tgcgcttgag ttttttcact tctttatcaa gcgcctggat ttcgtcttcg
     1261 ctcatcttcg cctccgtgtg cttacaggcc ggccacgtcg gaatatttgc cgatgcgctc
     1321 cagcgccacg gccagcagct tgtcggcttc gtccttcatt ttggacaggc tgtcaaagcc
     1381 aaagcggtgt acgtcgcgca gggttttgtc caccaccacc agcttgccca cggtgatcag
     1441 cgcgcggcca aagccttcgt gggtcaggtg gatcagcggc acggccagca ggcggcattc
     1501 tttttcgatc agcacggcga tggcgttgta cagcgccttc acccgcgcca gggtttcttc
     1561 gtccgggtcg cccaccagcg ggatttcctg acggcgggcg cgggtcagca cgaagggatc
     1621 gaggattttt tccgggcttt ggccgttgta gacgccgtag gtgtccagcg cgcgcatctg
     1681 gcgcaccatt tcgcggatga agtcagtgcc caggatcggg tcggattctt gcacaacagg
     1741 ggcggtgctc atggtggggt ctccagtggg gtgtcagtgt gaaagcacgt tgggctgcgg
     1801 cgtggcttca tgagtcgggg tggcggcgct gacttcgggc aggcgcgccg gggccagtcg
     1861 ggccagcaac agggcaaaca gaaaaccagt cagtgcgccc accgccacgg cgacatcgcc
     1921 gccgatggca aagtggccca gcgccgcgct cagcaacatc atcaccggcg gcagcaaata
     1981 gcccatgaag gcgctgcggc gcaggctgtc ttcgctgatg cgcacgcgca ccgattcgcc
     2041 cacttgcagg ccgctgggcg cgggcagggt cagccgggtg acggcgcggc cgccagccac
     2101 ctggccgatg ccgcattcgc tttctttgtg gcagctgcgg cagccgccag tggccacgct
     2161 gatggtggtc ttgccgtcct ggacggcttc gacaatgccc agatgttcga tcatccttcc
     2221 cacccttctt cctgcatggc ggcaaagcgg tcggcgctgc gcgccgggcg ggcgtgttcg
     2281 gccatggcct ggtcgatcca ggccaccccg ccgtgcttca tgccctgggc gatgtcgtag
     2341 agcaggtctt cgattttgtc ggattcggtg atgcgaatcg ggcggatgcc gcgcgccacc
     2401 agctgtttga tcgccgaggc gccgatggcc atcacgaaca ccgccgcgca gccctccaga
     2461 aaatccacct tggcgcccag cttgttttcg ttgccgtcca tggcctcggg ggcgaactcg
     2521 gccacgccca ccatgctggt gcgggtgggg ctgacctcgt agagcacaaa cccttctgcc
     2581 gcgccaaagt gctggttgac gcgcgtgcgg tcgttggaag caaaagcaat cttgagcatg
     2641 tcgtgtgtct cccggtcagt ggaccattcc agcttcagcc agcgcgaggg gcgcgccgtc
     2701 gcgttctgct gcccagtacc gggatcggta gggatcaatg tcatggtgct ggctcaacat
     2761 caggttggcg agatcgaaca gggcttggcg cgtgccgtga tagcccaccc aggcgcgggc
     2821 gaaaccgccc acccagtcat actgcggaaa gccggcgcgc atcagcggcg cgcccaaacg
     2881 ctgggccgtg ccggcggcgt gcgaattgga caccaccagc tgcacgcgct gaccgcgcgc
     2941 cgccagctcc aggtcttcca ggtcgccgac aatcaccttg gccaccggca ggcgcgccag
     3001 gccgtcggtt tttaccggcg acacggcggc aataatctcg gcgcccatgc tggccagaaa
     3061 ctcgcagtgc atggccagca gatcagggtc gcccgccacc gccacccgcg ccatgcccag
     3121 catgaaatgg ctgtccacca tggcgtcctg caattgggcg cgctggcgct ccagacgcgg
     3181 cggcacgggc ttgccggaca gctggctgag ggtgagggtg aatgcatcgc aggccgccaa
     3241 ccccattaat ccggcaaagc ggtaggtcgg cacgcccgtg cgcgcttcca gcttgctggc
     3301 ggcgcgatac agcgattcgc caatcaccag ggtggccacc gattcgccca ggctggcgat
     3361 ttccgcgcgc ggcgtgccgc ccagggtcag cggcgaatat tcggcctcga ccagatggcc
     3421 atccagcgaa tcgccaatat caggcaccag caccggccgc aggccaaagg cttcgatcca
     3481 gtcgcgcaga gtttccagat cgcccggcgt caggtggccg cccgccagca cggtgacctg
     3541 cttggggcgg cggccggcgt ggcgggtgtc tggcgccagg gtgtccagca gcgcttccac
     3601 cgccagcgcg tagccggttt ccaggcagcc cagcgtatcc ggggtgttca ccgccaccac
     3661 gctggtgtcg gcgtactgcg gataggccgc gcgaaactcg cgcacacagc ggtgaatatc
     3721 cgtgccctgg gtttctgaca gcccggtggt gaccaggccg atgatctcgg gcttttgctt
     3781 gtcgcacagc gtgcgcaagg cctcgatcac gttctcgtcc gcgcccatga tggtggacac
     3841 ctggtccatg gccgtggtct gcaagggaat cggctcgcga aaatggcgca cgaaaaacac
     3901 cttgccaaac gcggtgcaac cctgcgagcc atgcatcagc ggcaggcttt tgttcaagcc
     3961 gagaaacacc agcgatgcgc ccaaaggctg gctgaccttc agcggattca ccgccagggc
     4021 ttttttcgac agtacgattt cagccatgct gacctccctc ggtgatgttg ccaggagtgc
     4081 agcaaagaac gcgccaatag aatatgtatt gaataacaat ggcttggcta attcatctgt
     4141 cgcaaaccag acaaaatgtg catgggagca cacgcagcca aacaaggctc aggagaatgg
     4201 aaaaaatgca aacggggccg ggcgatcaag cccggcccct cggcgcggca cacacagcgt
     4261 ccgtgcagac ttaacgccgc ttacgcgctc agcagctcgc ccagcgcccg gcggctatcc
     4321 gcccagggcg ccggttggcg cacggccggc cacaccgggc tgctgatgga cagatgcagc
     4381 tggcgcgcca gttcaaccat gccggtgtag ccggcgtagc caaattcgcg ttcctggttg
     4441 atgtcgagga agggaatgcg cgccttcagc gcggtgtaca gattgcgccc gccggcaatc
     4501 aggatgtcgg ccttcaggtc gtggtaggcg gacagcaggc ccttggggct gccgtcgtcg
     4561 atcatgcgca catcgtcgcc catcagctcg cggatgcggg ccttgtcttc ttcggtggat
     4621 tttttggtgc cggtggccac cacggtcatg cccaggtctt gcagcgctga gacaatcgac
     4681 caggatttca ccccaccggt gtacaagagc gcgcgcttgc cggccagttt ttcccgccac
     4741 ggcgccagcg cggcattggc cagcgcttct tcgcgggcaa tgaccatttc ggtgcggtgc
     4801 gacaggtcat catcgccaat caggcgggca aattcgcgca gtgctgccga ggtgtcggcc
     4861 acgccgtaaa aactgccttc aaaaaacggc gtgccaaaat tgtcttgcag cttgcgcgcc
     4921 acgttcagca aggccttggc gcacaccacc atattggcct gggcgcggtg catggtctgc
     4981 acctcggcaa accggccatc gcccgacaag gtcgccagaa tgcgcaagcc cagctcgtca
     5041 aacagcgggg cgacatgcca gaactcgccg gcaatattgt attcgccaat caggctgaca
     5101 tcataggtgg gcaggccgtc cgggcgcggc gcggccggct gcggctcgcg cgtgccgatc
     5161 acatacttga acattgcttc accggccagg cggttgccca ggtttttggt gccgtaaaag
     5221 ccggcggcgt ccaccggaat caccggcacg ccccaaaaat cggtcgctgc cttgcagacg
     5281 gcttccatat cgtcgccaat cagcgcggtg acgcaggtgg cgtagacaaa caccgccgcc
     5341 gggcggtaac tctccaccgc ctgacgaatg gcgtgcagca agcgcttttc gccacgcccc
     5401 atcaccacat cggtttctga cagatccgtg gtcatgccga tgcggtacag cgtggggccg
     5461 gaagaccggg tgccgcggtt gtcccacgac gccccggcac aggcaatcgg cccgtggacg
     5521 atgtgggcaa cgtcggcgat gggcagcaag gcaatctgcg cgccatcaaa cgaacaaccg
     5581 cccgccgttg cgccgggctt gggtcgggcg caaccggatt tttccttgtg gttgtggcta
     5641 caggctggct cgttgagcaa agcctgaata tcgcttgctt tcattgcgca tctccccttg
     5701 agttcaccct agggcatcgc aattcgcatg ccacatttaa ccatttgata tttatcaaat
     5761 atttgccttg tcagctttgt ggcgaagctg acaaagcgtg gcagcccagc caaaaaaccg
     5821 cgcgcaggcc agtggttcat gcaagccaca gcggccaagg cgttgttatc gctggccgct
     5881 gtgatgagag ggatcggggg caactgagcg ccgccccccg gcaaacgcag atttagcgtc
     5941 gcgcaccaaa ctcggcgcag cgctccgggc caggcgcatg accgcagaca gtgccagcag
     6001 tacggcaagg tcgggcaagc acgccaggag ggtttggtga gtgacgctta cagggcatac
     6061 ggccgatgca gccaatcgcg caccaccgcc agatcccgct cgggcagata ctcaaacccg
     6121 gcttgcgcgt cttcgatgat ggtttgcgcc acccagtgcg gcacggcgtg ggtcaccagc
     6181 atatggaaat accaggccat cggataaccg caggcgcggt cgtaggcggt cagcgcggcg
     6241 cgcaaggcgc cgttggtctg gccttgttca ccgtgaaagg ccggcggatg gccttccagc
     6301 gtgctcatcg gcttggcgcg gaacaccgac tgcaagaagc ggtcttggtg ttcgctcatc
     6361 gccagcaccc agtgattgca aaaatcactg gcctggccgg cgctgcacgc cgcccagtct
     6421 tccagcatgc ggtggatggg gccgggttcg gggtgtttgg cgcgcatgcg cgccacaaag
     6481 gcgccggcat ggctgacgcg gcggaacaca taatacggca ggcccagcgg acggccgctg
     6541 tgggcgggca gttgcgggtc gagcagttcg tccagggtgg ccggttcgcc atcggcaaac
     6601 agggtgtggc tggcgatttc ctgtaattct tcgatttcca gttgcaaaaa atccgccggg
     6661 ccatcgccag cctgcgccac cagataatgg gtgcgcccca ccagccgggt ggccagcaac
     6721 ccttgttcgg cgtagtcctc ggccagccag cccagatcac cttggcgagt cgccagctcg
     6781 gtttccgccc agctttccag attgtcggtc aggcgtttgc ccttggcgtc gatcagcccg
     6841 cccagccggt gccagccgcc ccggcaggca gcgtgacgga aaggccagtc tggcgcggtg
     6901 ttggccagtt cgcgcaccag cgccgacggc ccggtggaca ccggagccat ggtgcaggcg
     6961 tgaatcaggg ccggggccag ggatttacag tattcggacc aggcagcggt atctttcatg
     7021 cggcgttcct tgtcgcagtc tcaacactct ggcgcccgcc tcatctggcg gccgccaaac
     7081 caaaaaacag tcggaatcag cgtcgcgaca gcgcatgcgc cggcggcttg tcctgcggtt
     7141 ttgcccagcc caccgcacag gatgggctgg gcaaaaccgg ctcgccgaca ggcaagccgg
     7201 aaaacaacaa tttttttagc gcggcctaag agtcgctcac aaaacccagc gcagcgcgcc
     7261 tggccaggcg cgcgcagaca gtgcaagcag tacggcaagg ccgcgcaaca acgccaggag
     7321 ggttttgtga ccgacgctag tcgtcgtcgg ccacgcggct ggccaccagc acgccggtgg
     7381 cggcgtcggc gctgtgggtc agcgacaaac aatggccgct ggcgccagac accagataaa
     7441 tcagatggtc ggtggtttcc cgcactgggg tggcgcgcac cggcacgtcg tcttcgctgc
     7501 attcggtaaa gtgcaggcca ggaaaacagc cgcgcagatg ggccacggcc gcgtttacgg
     7561 ccacttcggc ggtcagggcg tccagggtgt cggtggcgat catggttcga tttcctccat
     7621 gtcggcttca aacttggcca gcgaatgcgc cggcacgccc atgatttgcg ccagccacgg
     7681 cggcggcgcc aacaggcggc cttgcagctc ggccagcata tcgcgcgctt cgccgccaat
     7741 cgggcgcttg accgggtgca cgccagcgcg caccactttg gcggcggcgg ggccgccgat
     7801 ggattgcacg cccagaatct ggcagtcgtt gatcagctcg gcgcgcgcgg cgtttttgtc
     7861 ttcggcgtcg gcggtggaca gggtgctgcg cacgcccacc aggcggattt cgctcgggct
     7921 gacttgataa atcaggaagc gctcgcacga gccaaaatgg ccgtcgagca gttcattgtg
     7981 attggaggca atcgccacgc gcagcgagcc gggcatgtcg ccgtcggcat aggcttgcac
     8041 aggcggaaaa tcgctgccgg ccacgccttc gccccacaac aggcggatgg cggctttcag
     8101 ggtgtcggcg tccacgccga catggcagct ttggtcggcg taatcgccgg ccaggagcgc
     8161 gcgcaggtct tccacggtca actgcgccag gcggtcttcg gtgatcggca gcccgcattt
     8221 gtcggccagg gcttcgacca gggcgcgaac attgaggttg ttcagggtgc gcgcggcaag
     8281 ggcgatgcgc agcgcggctt cacgagaggc gatcggcgta ttggacatga cgggctcctg
     8341 gagcgaagag acagacggtg agaatcaagc cgggccgata cagccggacg ggcagacgtc
     8401 cacgcacagc ggggtgtcgt tgtggccgtc gcactcggta caggtggcgg cgtcgatctt
     8461 gtagaacacc ttgccgctgc tgatcgattt ggtcgggcaa accggcttgc agtcgccaca
     8521 ggcagtgcac atatcggaat cgatggtcat ggccatgatg aaatcctcct gaaaaacacg
     8581 gtgtgtgaaa aattcagtct tcggcagcgc ccaggcgttt ggcgcgcagc gagatcggca
     8641 ggcgcggcgg cttggcgatc gggtcgatgt agtagctgcc gccgttatcc agcttgagtt
     8701 cgccgcccca tttttccggg gtgtcgaatt cgatggattc gatggacgct tccaggtcgc
     8761 gcttgggcag ataaaacgac agcccgccat tttcggcttg gcggatcatg atgttggcca
     8821 tggggtcggc tccttgaatg gcgggtgagg cgggcgcgcc gcccgccgga taaccggcgg
     8881 gcggcaggac tgcgcttcgg gggctggatc agcgaaccag gtcgaagttg tagtcagtgg
     8941 tgcccatgcc gagggtttcg cggtccagct gctccagcac ggcattcacc agggtggtga
     9001 ccacgtacat ggcgccttcg tagcccatgg tggtcatccg gtgcaggtga tgacggtcga
     9061 agatcgggaa gcccaggcgg atcagcggca cttcgaactc gtcgcccttg tgcttggtgt
     9121 cgcgctggat gtacttgccg tagctgttgc caatcaggaa gtccggcttg tcggtgaagc
     9181 acaggctgcg caggtgccac aggtcattgc cgacatacac cttgccgttg acgccgaacg
     9241 ggctggagtc cagcagcttt tgcacgccct tggcccaacg cttgttgccg ttgtgcgcca
     9301 ggatgtgggt cggctcggcg ccgcattcca gcaggatctt gaccatgccc agcacgaagt
     9361 ccgggtcacc gtacacggcg aacttcttgc catgcagcca ggcgtggctg tcggagatca
     9421 agtccatgca gcggccgcgt tctttggcca gcgaatccgg aatcggcttg ccggtgactt
     9481 ccgacacctt catcagcaac tcgtcggtcc agtccacgcc catcgggatg ttcagcttgg
     9541 gcacgtcgtg gttccaggtg gtttcgacga atttcttcgt cttttccagg tgccacggtt
     9601 gcagcagcag ggtggtggcg gcgttcggcg cggcttccac ttcggcgatg gtggtgccgc
     9661 cagcgtacat gcggtattcg ccatcggccg gggtgtccag cacttcggtc gggtcggaca
     9721 gcatggtgta atccacgtcc atctcaccca acatgcgctt gatcacgcgg tagttgccca
     9781 ggtaggtttc aaagcccggc accacgttga ccttgccgct ggagccgaca accttgcctt
     9841 ccatcttgtt cagcgtgaag tagcgcagga tgccttccat catgttgtcc cagccggtga
     9901 catgcgaacc gacgaacgac ggggtgtggg caaacggcac cgggtagtct tgctcgacgt
     9961 ggccggcctt cttggcgttg ttgatgaagg cgttcaagtc atcgccaatc acctcggcca
    10021 tgcaggtggt ggacacagcg atcatggtcg gcttgtacag cgccttggcg ttttccaggc
    10081 cgtcgaacat gttcttctgg ccgccgaaca cggctgcgtc ttcggtcatc gagtcggaca
    10141 cgcaagcgat cggctctttg aagtgacggt tgaagtacga gcggaagtac gccacgcagc
    10201 cttgcgaacc gtgcacatac ggcagggtct tttcaaagcc cagcgagcac agcacggcgc
    10261 ccagcggttg gcaggccttg gccggatcga cggtcagcgc ttcgcgctgg aagttcagat
    10321 cctggtattc cttggtggtg gtccacagga aggtttcgta caccttgtct gcgccgtggc
    10381 cttcttcgaa caggtcgcgc ttggccttca gcgaggcttt gtaatcgtca tcgccgaaca
    10441 gcggatagca cggtttgatt ttgtcaacag tttgcatcat ggtctccctg accttcgcca
    10501 ctcatccgtg gcgaaggcaa agttgggcgg gggcgaaaat caggcggctt ttttctcttc
    10561 ggcgctggcc ggagcagtct tccacggcgg ggtcagcttg ctccacaccg ggctggacag
    10621 agcgatgtcc atgtcgcggg cgaagatggc gaagccgtcg tagccgtggt acgggccgga
    10681 gtaatcccag gagtgcatct ggcgcagcgg gatgcccatc ttctggaaga tgtacttttc
    10741 cttgatgccg gagccaacca ggtcgggctt gattttcttg acgaattctt ccagctcgaa
    10801 gccggtcaca tcgtcataca gcagggtggc gtcgcccatt tctttcaggg tgcggtcgta
    10861 gtcatcgttg tgggcgaatt cgtagcccgt gccgatcact tccatgccca gatcttcgta
    10921 ggcgccgatc acgtgacgcg gacgcagacc accgacgtac agcatcactt tcttgccttg
    10981 caggcgcggt ttgtacttgg cgacgatttc gtccatcatc ggctgataac gggcgatcac
    11041 ggcctcggac ttttccttga cggaatcatc aaagaaggcg gcaatcttgc ggatcgattc
    11101 gatgatcttg gtcgggccga agaagttgta ttccatgtaa ggaatgccgt acttctcttc
    11161 catgtggcgg ctgatgtagt tcatcgaacg gtagcagtgc agcaggttca gcttgacctt
    11221 gggggtgttc atcatttccg ccagggtgcc gtcgcccgac cactgcgcca ccacgcgcag
    11281 acccatttct tccaggatca cgcgggttgc ccaggcgtcg ccgccgatgt tgtagtcgcc
    11341 gatgatggcc acgtcgtacg gggtggactc ataagcctga ccgtcgcgct tgtccagcac
    11401 ccagtcacgg atggcgtcgt tggcgatgtg gtgacccagc gactgggaca cgccacggaa
    11461 gccttcgcaa cgcaccggga taatcggctt gttgatgacc acggcgctct tcttggagac
    11521 ggcttcgatg tcatcgccaa tcagaccgat cgggcattcg gattgcaccg agatgccgtt
    11581 gtgcagcggg aacagttgtt ccacttcttc aatcagcttg gtcagctttt tgtcgccgcc
    11641 aaacacgatg tctttttcct ggaaatcaga agtgaagttc atcgtgccga aggagttcac
    11701 cccggtggtg ccgacgtaat agttgcggcg accagcgcgg gagtactgac cacaccccac
    11761 cgggccgtgg gagatgtgga tcatgtcctt gatcggaccc caaaccacgc ccttggagcc
    11821 ggcgtaggcg caaccacgga tggtcatcac gcccggcagg gacttacggt tggaggtaat
    11881 gcactttttg gactgttcca gcgaggtgtc gttggcagcc aggtgctttt tgcgatcttt
    11941 cttggctttt tccgggtaaa cctccagcac ctcagagatg agggcttcgg tttgttcacg
    12001 cgtcatgctc atagtggcct ccgtcgcgtt gccaccaatc tgtggcgaaa cgcttgtgtt
    12061 gaaggggcag gcgccagggc ggggggccgc ctgccgggtc gcagagtcag gtgcaggccg
    12121 gtcgggcaat ccgccaacct gcggtacagc gcattagcca gcggccagtt cggcggcggt
    12181 cttgccgacg atggactcgt cttcggcttc catgatgccg aattccatca gcagctcttc
    12241 cagctcttcc atctcgatcg gggtcgggat gatgaagttg gtgttgttga cgatcttgtt
    12301 ggcgagcgtt cggtattcgt cggcctgttt gtgcttcgga tcgtattcga ccatggtcat
    12361 gcggcggatt tcagcatgct gaacggcgtt gtcgcgcggc acgaagtgga tcatggtggt
    12421 gcccaggcgg gaagccaggg ccatgatcag ttcgtcttcg cggtcggtgt tgcggctgtt
    12481 gcagatcagg ccggccagac ggacgccgcc ggagttggcg tacttcacga tg
//
LOCUS       DAOPYX010000046        12520 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_5624_length_12520_cov_9.480947, whole genome shotgun sequence.
ACCESSION   DAOPYX010000046 DAOPYX010000000
VERSION     DAOPYX010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 12520)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12520)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12520
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5624_length_12520_cov_9.480947"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            73..774
                     /locus_tag="PKN55_03870"
     CDS             73..774
                     /locus_tag="PKN55_03870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019923990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNX70413.1"
                     /translation="MMVHQPESAATLVALQSVSQCYPGRQGMVKVLDEVSLSVCQGDF
                     IAITGESGSGKSTLLNIIGLLMPPSSGVVNYQGRPVSFGRGAEVAQRAAGRPIGFVFQ
                     QFNLIPSLSVLDNVALPLLYQGLAPAARAAQSLAVLETLGIADKAGQRPDDLSGGQKQ
                     RVAIARALVAQPALMIADEPTGSLDPRHADEVMTLLCELNQAGMALVMVTHDLALAAR
                     AKSRWRIAAGRIVHD"
     gene            767..1933
                     /locus_tag="PKN55_03875"
     CDS             767..1933
                     /locus_tag="PKN55_03875"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNX70414.1"
                     /translation="MIKPRTLWLAAEALRNIQFSRTRIVLTLVGIVISTASVVAILTI
                     GWNAATESVRRFSSLGANLFVVSNLEQGQVALYADMEAEKQALRARFPFVQGAAWVFS
                     LSSRAKGGARQADAVLYAGDAQFRPMVSPQWRQDGPSAGVWVGAGLLAELGLPARPGP
                     DVGLRLEGRYYPVAGVFAELDNIPFVDIDARNAVILPASATRHLFPGPAFARMLIRAP
                     AAQVNESTASALRGYFVQRGFVAAEVNYPAKIIESLKTQQQLFAWLLAGVALISVVVA
                     AVGVMSSTLSDLADRRREIAIRLIVGASVRDIFWMLSVEALALSLLGAAGGVVVGLLC
                     AALAAWLAGWAFAVHGGYVLLAVLCSSGICLGFSLFPVRYLMRPSIVEALRAES"
     gene            complement(2294..3685)
                     /locus_tag="PKN55_03880"
     CDS             complement(2294..3685)
                     /locus_tag="PKN55_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012698030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HNX70415.1"
                     /translation="MTDLAARLAAVIDPARIRTDADSLQQYGRDWTRYYAPAASAVVF
                     PHSLGEVQALVQLARAERLALVPSGGRTGLSGGACAVAGEVVVSFEQMREISDFDPVA
                     ATVRCQAGVVTESLQQYALAQGLYFPVDFASRGSSCIGGNIATNAGGIKVVRYGMFRD
                     WVAGLTVVTGAGDVLRLNQGLVKNNSGYDLRHLFVGSEGTLGLIVDATLRLARAPAPA
                     RVMVCAAPALEHILQVFHAFRARLTLNAFEFFSEAAQQAVLARGHVRRAVDTVTPYYA
                     LLEFDAEHEPAALAAFEACVDAGWLADGVISQSDTQAAALWRLREDISESITPRTPYK
                     NDVSVVISRAPAFIAELDALLAREYPDFDVVWFGHIGDGNLHINVLRPLDMDIDTFRG
                     HCERVNSLVFDVLARYQGSMSAEHGVGLLKRDYLPVTRSAEEIHYLRGIRQVFDPDGI
                     LNPGKLLPAAESA"
     gene            3797..5026
                     /gene="serA"
                     /locus_tag="PKN55_03885"
     CDS             3797..5026
                     /gene="serA"
                     /locus_tag="PKN55_03885"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014086705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HNX70416.1"
                     /translation="MQSYSLDKDKIKVLLLEGVHQTAVEAFQASGYVTLDYHAKALPE
                     AELCAAIADAHIVGIRSRTQLTETVFAAARKLMAVGCFCIGTNQVDLAAAARRGVPVF
                     NAPFSNTRSVAELVLAEIIMLMRGIPQRNALAHRGGWLKSADHAYEVRGKTLGIVGYG
                     HIGAQISVLAENLGMQVVFYDIETKLPLGNARQLPSLEALLGVADVVTLHVPETEQTR
                     HLIGAAELAAMKPGSHLINASRGTVVDIDALAAALHSKHLHGAAIDVFPLEPQANGDE
                     FLSPLRGLDNVLLTPHIGGSTQEAQANIGAEVASKLIRYSDNGSTLSAVNFPEVSLPA
                     HADSCRLLHIHRNQPGMLARINALFSGQNINIRAQYLQTSGDLGYVVIDADSAASSTA
                     LAMLQAIEGTLRCRVLY"
     gene            5123..6835
                     /locus_tag="PKN55_03890"
     CDS             5123..6835
                     /locus_tag="PKN55_03890"
                     /EC_number="2.7.7.65"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HNX70417.1"
                     /translation="MTQPDPFHDPLSRAFGSAELAGIVQFCNQASRADVCAAVVRLVA
                     QLRDDEVTLPGFPPRRACPGHPGQCQPDFHAALTSLESELTQTQASLARLSAAVASLR
                     ARTPDWQGGASPDELAAGLEHWLHRQPGHAIQQPPQARNAPYAAADLAVLLPARLATG
                     KPFSLLCLSLDHFASLRLRHGAALAERLLSQLGQRLLAHARHDQVAHLGQDEFAILSE
                     HIDDSAGHAARRVLQRVGIELKIDGERFALSASVGVSQFPADGEDAETLLLRARQATA
                     AARRAGGATWARYHAEHTPAQQAREALENDLRLALGRPDEMHLHYQPIINFRTGQVVC
                     MESLARWQHPRLGMVSPERFIPIAEESSLIVALGHRLLDTALAQWQSWRQAGLPVAPV
                     SVNLSGRQLCKPDLADDVAALLHRHQMPPAMLQLEITETALAQAGAAPISRTLDALKA
                     LGVSIALDDFGCGYANLQQLYRFRPEQIKIDRSFISKIGLDPHSEVIVAALLSLSQNF
                     GVGVVAEGVETLEQANWLWRHGCDVLQGFLFSRPLPATEMAAYLRDLPQSRAMCPLGE
                     RAPM"
     gene            complement(6848..7759)
                     /gene="gluQRS"
                     /locus_tag="PKN55_03895"
     CDS             complement(6848..7759)
                     /gene="gluQRS"
                     /locus_tag="PKN55_03895"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007341526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HNX70418.1"
                     /translation="MNAPSFQDLPASAYRGRFAPSPTGLLHAGSLTTAVGSYLHARSQ
                     GGQWLLRIEDLDPPREVPGAADAILRTLEALGFTWDGPVIWQSQRLTAYQDALDALIA
                     SGHAYPCACTRREIALAGHPGVDGVVYPGTCRNGLTAGKAGRAWRLHVPAGEVMFMDG
                     HYGAQRQDVAHQVGDYVLKRADGLFAYQLAVVVDDAWQGISEVVRGADLLDSTPRQIV
                     LQRALNAPTPRYTHLPLLVNAAGEKLSKQTLAPAIRPDQAADELRLALTRLNHAPPAD
                     CQGLDELWGWALAHWSLARAPAAPVPV"
     gene            complement(7814..8434)
                     /locus_tag="PKN55_03900"
     CDS             complement(7814..8434)
                     /locus_tag="PKN55_03900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012696052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemoreceptor glutamine deamidase CheD"
                     /protein_id="HNX70419.1"
                     /translation="MPIPLSFLQNPLPREVYFDKHFGLEATKLWPGEILATREDWLLV
                     TVTGSCVVLCLHDPVAAVGGMAHFMVPDLEAGATDTHAPARYASHIMESLIDAMLLQG
                     ARRERLTALLYGGANILKGLYLGAPGERNVLFTRNYLSQEGIPLHDEAALGMTPRKIY
                     FQPKRGRTKIRGIREAHNRTIFERELEYRHRLRELQLPHEGEVFDL"
     gene            complement(8460..8849)
                     /locus_tag="PKN55_03905"
     CDS             complement(8460..8849)
                     /locus_tag="PKN55_03905"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNX70420.1"
                     /translation="MPSPQAAPADILLVDDSMCCRQSIGFALRRAGHQVREASSADAA
                     LRELERTGPPKVCIVDHIMADLDGACLIHILRQRPDTTHTAMVLMSTVETNYLRALAR
                     EVGAHAWLAKPFAPEQLLAIISILLDR"
     gene            complement(9187..10377)
                     /locus_tag="PKN55_03910"
     CDS             complement(9187..10377)
                     /locus_tag="PKN55_03910"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNX70421.1"
                     /translation="MRSHDATGSTPQTTGLEHAFDRFTQVSGQLIDAYLALQQQVSHL
                     SEQLALANEQLSRRVAENTALLGRLSLLLNMLPAGVIEIDAEGLVSRQNRAASALLGE
                     DWSHRPWEACASSFGHAPHDDGQVSYHTPQGELRHMLIQRQPLPDDDGAIILVQDVSR
                     ALEMQQQLAQQERFVAMGRMAASLAHQLRTPLATAMLYTSHLNRPSIDDADRLKFAGK
                     ALTRLRALEGLVSDMLGFVRGHVEAREWVALPRLLADAEQVFAPQAQAREVVLDWPAQ
                     APQVELYSDGKALLGAFVNLLENALVFSPPQGHIRVSTELGNGVLKLTVQDDGPGVSA
                     EVAPRIFDPFFTTRADGTGLGLAIARGVAEVSGGELTLQASPRGACFRLQLPCRVMPQ
                     EPAD"
     gene            10536..11531
                     /locus_tag="PKN55_03915"
     CDS             10536..11531
                     /locus_tag="PKN55_03915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011136993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein"
                     /protein_id="HNX70422.1"
                     /translation="MSELLKKIDARTKLAGTNKLEILLFSLGTDQRTGRRETFGINVF
                     KVREVMRTPEITAAPEMTASVEGMVSLRGQLVPVIDLAKYTGIVTETKPDIMIVTEYN
                     GHTQGFLVEAVDTILRLDWSLMRVPPEMISNRMGGLVTAVTELNDQTLVMMIDVEKVL
                     AETTQIDDSYQFVNIEPVSVPRMVFYADDSAVARKQIERTLDAMGIRHAGAINGRQAW
                     EELQRLATQAEMAHRPLNEMLHLVLTDVEMPEMDGYILTKMIKTDIRFAGIPVLMHSS
                     LSGHSNQKLGQSVGVDEYVSKFEPHKLAQKVRQLLKLDAPQSTLLPSGQATLPRD"
     gene            11566..12501
                     /locus_tag="PKN55_03920"
     CDS             11566..12501
                     /locus_tag="PKN55_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008954976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein"
                     /protein_id="HNX70423.1"
                     /translation="MTASDASLLANIDARTKLAGSNKMEILLFTLGTREIFGINVFKV
                     REVSQTPEITKTPNMPMGVQGVISLRGNIIPVIALDKFIGRSKPDNVYASMIVTEFNK
                     STQAFLVGEVDRIIRVDWDKVRAPENMVSTSQQAMITAVTELEDGKLVSILDVEQILA
                     SVIGETRIPDIPKANLDADQFIFFCDDSVVARKEITGMLDKMGVKYHQANNGREAWDR
                     LQAFANRQWNEGESLHDTLKLILVDAEMPEMDGYVLTRLIKNDPRFADIPVVMHSSLS
                     SNANRAMGTSVGVDAYVAKFEPALLAETMLPFLQR"
BASE COUNT         2274 a         3715 c         4060 g         2471 t
ORIGIN      
        1 agccgcacat gtccaaggtg ctggaaaccc tgctgattgc cgagcgggca gaggccgcac
       61 aacgttgatg tcatgatggt gcatcaacct gagtctgcgg ccaccctggt ggcgctccag
      121 tcggtcagcc agtgctatcc aggccggcag ggcatggtca aggtgctgga tgaggtgtcg
      181 ctgtcggtgt gtcaggggga tttcatcgcc attaccggtg aatctggttc aggcaagagc
      241 actttgctga atattattgg cttgttgatg ccgccttcgt ctggcgtggt gaactatcaa
      301 gggcggccgg tgagtttcgg tcggggcgcc gaggtggccc agcgtgctgc gggtcggcct
      361 attggctttg tgtttcagca gttcaatttg attccatcac tgtccgtgct ggataacgtg
      421 gccctgccct tgctgtatca ggggctggcg ccggctgcgc gggcggcgca aagcctggcc
      481 gtgctggaga cgctgggcat tgccgacaag gcggggcaac ggccggatga tttatctggc
      541 gggcaaaaac agcgggtggc gattgcccgt gccttggtgg cgcagcccgc gctgatgatt
      601 gccgacgaac ccaccggcag cctggaccca cgccatgccg atgaggtgat gaccttgctg
      661 tgcgagctga atcaagctgg catggcgctg gtgatggtca cccatgatct ggccctggcg
      721 gcgcgcgcca aatcgcgctg gcggattgcc gcaggacgga ttgtccatga ttaagccccg
      781 cacgctgtgg ctggcggcgg aagcgctgcg caatattcaa ttctcccgca cccgcattgt
      841 gctgaccttg gttggcattg tgatttccac tgcgtcggtg gtggcgattc tcaccattgg
      901 ctggaatgcc gccactgagt cggtgcgccg tttttccagc ttgggggcca atttgtttgt
      961 ggtcagcaat ctggagcaag ggcaggtggc cttgtacgcc gatatggagg ccgaaaagca
     1021 ggctttgcgc gcgcgctttc cgtttgtgca aggtgcagca tgggtgtttt ctttatccag
     1081 tcgcgccaag ggcggcgcgc gccaggcgga tgcggtgctc tacgcgggcg atgcgcagtt
     1141 tcgacccatg gtgtcgccgc aatggcggca ggatgggccg agcgcgggcg tttgggtggg
     1201 ggccggcttg ctggcggagc tgggcttgcc cgcccggcct ggtccagatg ttggcctgcg
     1261 gctggagggg cgttattacc cggtggccgg ggtgtttgcc gagctggaca atattccatt
     1321 tgtggatatc gacgcgcgca atgcggtgat tttgccggca tccgccaccc gtcatctgtt
     1381 tcccggccct gcttttgccc gcatgctgat tcgggcgccg gcagcgcagg tgaacgaatc
     1441 caccgccagc gccttgcgcg ggtattttgt tcagcgcggc tttgttgccg ccgaggtgaa
     1501 ttatcccgcc aagatcattg aatcactgaa aacccaacaa cagttgtttg cctggttgct
     1561 ggccggggtg gcgttgattt cagtggtggt ggcagcggta ggggtgatga gcagcacgtt
     1621 gtctgatttg gcggatcgtc ggcgagaaat tgccattcgg ctgattgtgg gcgcctcggt
     1681 gcgcgatatt ttctggatgc tgtcggttga agccttggcg cttagcttgc tgggcgcggc
     1741 gggcggcgtg gtggtgggtt tgctgtgtgc ggcgctggcg gcttggctgg ccggttgggc
     1801 atttgccgtg catggtggct atgtgctgct ggcggtgctg tgctccagcg gcatttgcct
     1861 ggggttttct ttgtttcctg tgcgctatct gatgcgcccc tcaattgttg aagccttgcg
     1921 cgctgagtca tagcacgccg ttccttaaga gtcgctcaca aaaccccctg gcgttgttgt
     1981 gtgaccttgc cgtactaccc gtactgtctg cggtcacacg cctagccaag gccgcttcgc
     2041 tgggttttgt gagcgactct taaatacttg tccacgcgac agaaccgcac tgtccacacc
     2101 ccagggggac aggggtgtga accgcctgtg gacaaatcat ccagctggct gatttatcat
     2161 gacttttctg tgttgcctat tttttgggca gtggggtggg catccacacc ggcgcgggag
     2221 agaagtgtgc accggctgtg gataaagcag tttattgatt gatttttaag caaaaacttt
     2281 tgttgcacat tttttacgca gattctgccg ccggcagcag cttgccgggg ttgagaatcc
     2341 cgtcggggtc gaacacctgg cggatgccgc gcaggtagtg gatttcttcg gcgctgcgcg
     2401 tcaccggcag gtagtcgcgc ttgagcaggc ccacgccgtg ttcggccgac atactgccct
     2461 gatagcgcgc cagcacgtca aacaccaggc tgttcacccg ctcgcagtgg ccacggaagg
     2521 tgtcgatgtc catgtccagt gggcgcagca cattgatgtg caagttgccg tcgccaatat
     2581 ggccaaacca caccacgtca aaatccgggt attcgcgcgc cagcagcgcg tccagctcgg
     2641 cgataaaggc cggcgcgcgt gagatcacca ctgaaacatc gtttttgtac ggggtgcgcg
     2701 gagtgatgga ttcgctgatg tcctcgcgca gccgccacag cgccgccgcc tgcgtgtcgg
     2761 attggctgat cacgccatcg gccagccagc cggcgtccac gcaggcctca aacgccgcca
     2821 acgcggcggg ttcgtgttcg gcgtcgaatt ccagcagcgc gtaatacggc gtcaccgtgt
     2881 caacggcgcg gcgcacatgg ccgcgcgcca gcaccgcttg ctgggcggct tcggagaaaa
     2941 actcaaaggc gttcagcgtc agccgggcgc gaaaggcgtg gaacacctgc aggatgtgct
     3001 ccagcgccgg cgccgcacac accatcaccc gcgccggggc cggcgcgcgc gccaggcgca
     3061 gggtggcgtc cacaatcagg cccagcgtgc cttcgctgcc gacaaacaaa tggcgcaggt
     3121 cataaccgct attgtttttc accaagccct ggttcagccg cagcacatcg ccagcgccag
     3181 tcaccacggt gagcccggcc acccagtcgc ggaacatgcc gtagcgcacc accttgatgc
     3241 cgccggcgtt ggtggcgata ttgccgccaa tgcacgacga gccgcgcgag gcaaagtcca
     3301 ccggaaaata cagcccctgc gccagggcat actgttgcag gctttcggtc accaccccgg
     3361 cctgacagcg cacggtggcg gccaccgggt caaaatcgct gatctcgcgc atctgctcga
     3421 acgacaccac cacttcgcca gccaccgcgc aggcgccgcc agacaggccg gtgcgcccgc
     3481 ccgagggcac cagcgccagc cgctcggcgc gcgccagctg caccagcgcc tgcacttcgc
     3541 ccaggctgtg cggaaacacc acggcgctgg ccgctggggc gtaatagcgc gtccagtcgc
     3601 ggccatattg ttgcaggctg tcggcgtcgg tgcggatgcg cgcggggtca atcacggcgg
     3661 caaggcgggc ggcaaggtcg gtcatggtgg gtgagtccgt ggaggagggg ataaaaaagt
     3721 tttgcagggc tggggctggc aaaatccaga atacctgata aatttactca agaattcagc
     3781 aaaaaggaag cctcccatgc aaagctattc gctcgacaag gacaaaatca aggtgctgct
     3841 gctggaaggc gtgcaccaga ccgccgtgga agcgtttcag gccagcggtt atgtcacgct
     3901 ggattatcac gccaaggcgc tgcccgaggc ggaattgtgc gcggcgattg ccgatgcgca
     3961 cattgtcggc atccgctcgc gcacccagct taccgagacg gtgtttgccg ccgcgcgcaa
     4021 gctgatggcg gtgggctgct tttgcatcgg caccaatcag gtggacctgg ccgccgccgc
     4081 ccgacgcggc gtgccggtgt tcaatgcgcc gttttccaac acccgctcgg tggccgaact
     4141 ggtgctggcg gaaatcatca tgctgatgcg cggcattccg cagcgcaatg cgctggcgca
     4201 tcgcggcggc tggctgaaat cggcggacca cgcttacgag gtgcgcggca agacgctggg
     4261 cattgtcggc tatggccata tcggcgcaca gatcagcgtg ctggcggaaa acctgggcat
     4321 gcaggtggtg ttctacgaca tcgaaaccaa gctgccgttg ggcaacgccc gccagctgcc
     4381 cagcctggag gcgctgctgg gcgtggccga tgtggtgacc ctgcatgtgc cggaaaccga
     4441 acaaacccgc catttgattg gcgcggccga gctggcggcg atgaagcccg gcagccattt
     4501 gatcaacgcc tcgcgcggca cggtggtgga catcgacgcg ctggccgccg cgctgcacag
     4561 caagcatctg catggcgcgg cgattgatgt gtttccgctg gagccgcaag ccaatggcga
     4621 tgagttcctg tcgccgctgc gcgggctgga caatgtgctg ctcaccccgc acatcggcgg
     4681 ctccacccaa gaggcgcagg ccaatatcgg cgccgaggtg gccagcaagc tgattcgtta
     4741 ttccgacaat ggctcgaccc tgtcggcggt gaatttcccg gaggtctcgt tgcccgccca
     4801 cgccgacagc tgccgcttgc tgcatattca ccgcaaccag cccggcatgc tggcgcgcat
     4861 caatgcgctg ttctccgggc aaaacatcaa tatccgcgcg caatatctgc aaaccagcgg
     4921 ggacttgggc tatgtggtga tcgacgccga cagcgccgcc agcagcaccg cgctggccat
     4981 gttgcaggcg attgagggca cgctgcgctg ccgcgtgttg tattaaagcg gcgcgcggct
     5041 gctgcggcgc gccatcggcg acgctggcat gggggggaat cctcacttat gatgaggtta
     5101 ttgcatcgtc ggcggggtac gcatgacaca gccagatccc ttccacgacc cattgtcgcg
     5161 ggcgttcggc tcggccgaac tggccgggat agttcaattt tgtaatcagg ccagccgcgc
     5221 cgatgtttgc gccgccgtgg tgcgcctggt ggcgcagctg cgcgacgacg aggtgaccct
     5281 gccggggttt ccgccgcgtc gcgcctgtcc gggccacccc gggcagtgcc agccggattt
     5341 tcatgccgcg ctgacctcac tggaaagcga actcacccaa acccaggcca gcctggcgcg
     5401 cctgtcggcc gccgtggcca gcctgcgcgc ccgcacgccc gattggcagg gcggcgccag
     5461 cccggatgaa ctggccgccg gcctggagca ttggttgcat cgtcagccgg gccacgccat
     5521 tcagcaaccg ccgcaggcgc gcaacgcgcc ttacgccgcg gctgatctgg cggtgctgct
     5581 gccggcgcgg ctggccacgg gcaagccgtt ttcgctgttg tgcctgagcc tggatcattt
     5641 tgcctcgctg cgcctgcgcc atggcgcagc cttggccgag cgtttgctgt cgcaactggg
     5701 ccagcgcctg ctggcgcatg cgcggcatga tcaggtggct cacctgggcc aggacgagtt
     5761 cgccattctc agcgagcata ttgacgacag cgccggccac gccgcccgcc gggtgttgca
     5821 gcgggtgggc attgaactga aaattgacgg cgagcgtttt gccttgtcgg ccagcgttgg
     5881 cgtcagccag tttccggcgg atggcgagga cgccgaaacc ttgctgctgc gggcgcgcca
     5941 agccacggcg gcggcgcggc gcgccggtgg cgccacgtgg gcgcgctatc atgccgagca
     6001 cacccccgcc cagcaggcgc gcgaagcgct ggaaaacgac ctgcgcctgg cgctgggccg
     6061 cccggacgaa atgcatctgc attatcagcc catcatcaat tttcgcactg gccaagtggt
     6121 gtgcatggag tctctggcgc gctggcagca tccgcgcctg ggcatggtca gcccggagcg
     6181 gtttattccg attgccgaag aaagttcttt aatcgttgcc ctgggccatc gcctgctgga
     6241 cactgcgctg gcgcagtggc aaagctggcg tcaggccggc ttgcctgtgg cgccggtatc
     6301 ggtgaatttg tccggccggc aattgtgcaa gcccgatctg gcggatgatg ttgccgcctt
     6361 gctgcatcgc caccagatgc caccggccat gttgcagctg gaaatcaccg aaaccgcgct
     6421 ggcgcaggcc ggggcggcgc ccatcagccg caccttggat gcgctcaagg cgttgggggt
     6481 gagcatcgcc ttggacgatt ttggctgtgg ttacgccaat ttgcagcagc tttaccgctt
     6541 ccgccccgag caaatcaaga tcgaccgcag ctttatcagc aaaattgggc tggatccgca
     6601 tagcgaggtg attgtcgctg cgctcttgag tctgtcgcag aattttggcg tgggcgtggt
     6661 cgccgaaggg gtggaaaccc tggagcaggc caattggctg tggcggcatg gttgcgatgt
     6721 gctgcaaggc tttttgttca gccgaccatt gccggcgaca gaaatggcgg cgtatttgcg
     6781 cgatttgccg caatcgcgcg ccatgtgccc gcttggcgaa cgcgcgccga tgtgatcgcc
     6841 gtcagcgtca aaccggcacg ggcgccgctg gcgcgcgcgc caggctccag tgggccagcg
     6901 cccagcccca cagctcgtcc aagccttggc agtcagctgg cggcgcatgg ttgagtcggg
     6961 tcagcgccag acgcagctcg tctgcggcct gatcgggccg gatggccggc gccagggttt
     7021 gtttgctgag tttttcaccg gcagcattca ccagcaatgg caggtgggtg taacgcggtg
     7081 tgggggcgtt caaagcccgt tgcagcacaa tctgacgcgg tgtggagtcc agcaggtcgg
     7141 cgccgcgcac cacctcgctg atgccttgcc aggcgtcatc taccaccacg gccagttgat
     7201 aggcaaacag cccgtcggcg cgtttaagca catagtcgcc tacttggtgc gcgacatcct
     7261 ggcgctgtgc gccgtaatgg ccatccataa acatcacttc gcctgccggg acatgcagcc
     7321 gccaggcgcg gccggctttg cctgctgtca ggccgttgcg gcaagtgccc ggatacacta
     7381 caccgtctac tccggggtgg ccggccaggg cgatttcgcg gcgggtgcag gcgcaggggt
     7441 aggcatggcc gctggcaatc agggcatcca gcgcgtcctg ataggcggtc agtcgctgac
     7501 tttgccatat cacggggcca tcccaggtaa acccgagggc ttccagcgtg cgcagaatgg
     7561 cgtcggcggc gccgggcact tcgcggggtg ggtccaggtc ttcgatgcgc agcagccatt
     7621 ggccgccctg gctgcgtgcg tgcagatagc tgcccacggc ggtggtcagt gagccggcgt
     7681 gcaacaggcc ggtggggctg ggggcaaagc gtccgcggta tgcgctggcg ggcaagtcct
     7741 gaaaagaggg tgcgttcatg gccgtggttt taaccgaatc tggcctgttc tgccaagttc
     7801 aggacaacaa tcatcacagg tcaaatactt caccctcatg cggcagttgc agctcgcgca
     7861 ggcggtggcg gtattccagt tcacgctcga aaatggtgcg gttatgcgcc tcacggatgc
     7921 cacgtatttt ggtgcgtccg cgtttgggct gaaaataaat tttgcgcggc gtcattccca
     7981 gggcggcttc gtcgtgcagt gggatgcctt cctggctcag gtaattgcgg gtgaacagca
     8041 cattgcgttc gcctggtgcg cccagataca ggcccttgag gatattggcg ccgccataga
     8101 gcaaggcggt caagcgttcg cggcgggcac cctgcagcag catggcgtcg atcagcgatt
     8161 ccatgatgtg gctggcgtaa cgggccggtg cgtgggtgtc ggtggcgccg gcttccagat
     8221 ccggcaccat gaagtgggcc atgccgccta cggctgccac cgggtcgtgc aggcacagca
     8281 ccacgcaact gcctgtcacg gtgaccagca gccagtcttc gcgggtggcc agtatttctc
     8341 ctggccacag tttggtggct tccaggccaa agtgtttgtc gaaatacact tcacgtggca
     8401 gcgggttctg caagaaggag agcggaatgg gcatgatcgg ggccgtcgca gggtcagggt
     8461 tagcggtcga gcaggatgct gatgatggcc agcagttgtt ccggagcaaa tggcttggcc
     8521 agccaggcat gtgccccgac ttcgcgcgcc agtgcgcgca gatagttggt ctcgacggta
     8581 gacatcagca ccatcgcggt gtgggtggta tctggtcgct gacgcagaat gtggatcagg
     8641 caggcgccat ccagatcggc catgatgtgg tcaacgatgc agactttggg cggaccggtg
     8701 cgctccagtt cgcgcagggc ggcatcagca ctgctggctt ctcgcacctg atggccggca
     8761 cgacgcaggg caaagccgat gctttgccgg caacacatgg agtcatccac cagcaggatg
     8821 tcggcggggg ctgcttgcgg ggatggcacg ttcggtccac tccttcggtc tgtcgggttc
     8881 cgggaagatg gcgcgccgtg gcgtgggctg ggcggtctgg cgcaaatgac gtcatgccga
     8941 catcccgtgg tcatggtggc gtcatgccga cagtatagaa gcgttgccga tttttttcat
     9001 gtcattatcg tgggcaatga gatattgatt gctgaaatcg gcggtaaaca agcatcagcc
     9061 ttgcccagca ggacgaaaat ggctcataaa atgctgcaga gcagccaaat ccggcgtgtc
     9121 gtggggcagt cagaaccggc ctgcatgcgg gcaaaacgac gcaagctggc tctggcaagc
     9181 ggtgtttcag tctgctggct cttgcggcat gacccggcac ggcagttgca gccggaagca
     9241 ggcgccgcgg gggctggctt gcagggtcag ttcgccgcca gacacctcgg ccacgccacg
     9301 ggcaattgcc aggccaagcc cggtgccgtc ggcacgggtg gtgaaaaacg gatcaaaaat
     9361 ccggggcgcc acctctgcgc tcacgccggg gccgtcatcc tgcacggtca gttttagcac
     9421 gccattgccc agttcggtgc tgacgcgaat atggccttgc ggtggagaga acaccagggc
     9481 attttccagt aaattcacga atgcgcccaa caatgccttg ccatcactgt acaattcgac
     9541 ttgcggtgcc tgtgctggcc agtccagcac cacctcgcgc gcctgtgcct gcggagcaaa
     9601 aacctgctcg gcatcggcca gcaggcgtgg cagcgccacc cattcccgcg cctcgacatg
     9661 gccgcgcaca aagcccagca tatcgctgac caggccttcc agtgcacgca gccgggtcag
     9721 cgccttgccg gcaaatttca gccggtcggc gtcgtcgatg ctgggccggt tcaggtggga
     9781 ggtgtacagc atggcggtgg ccaaaggtgt gcgcagctgg tgcgccagcg aggcagccat
     9841 gcggcccatg gcgacaaaac gctcttgctg ggccaattgt tgttgcattt ccagcgcacg
     9901 gctgacatct tgcacaagga taatggcacc atcgtcatcc ggaagaggct ggcgctggat
     9961 cagcatgtgg cgtaattcac cttgtggcgt gtggtaactg acctgaccat cgtcatgcgg
    10021 ggcatggcca aagctgctgg cgcaggcttc ccatgggcgg tggctccagt cctcgcccag
    10081 caaggcggag gcggcacggt tttgtcggct gacgagccct tcggcgtcaa tttcgatcac
    10141 accggcgggc agcatattga gtaaaagcga taaacgccct aataatgcag tgttttcggc
    10201 aacccggcgc gaaagttgct cgttggccag tgccagttgc tcggacaaat gggagacctg
    10261 ctgctgaagc gccagatagg cgtcaatcag ctggccggat acctgggtaa agcggtcaaa
    10321 ggcgtgttcc agccctgtgg tttgtggcgt ggagcccgtg gcatcatgag atcgcatatt
    10381 tatttatgac gtttagcacg cttttttgtt tacaatagag atggaaaatc gtatcagact
    10441 gcccctggct tgccgaggca ctaccgctgg cctggtcaag aaaatcacgg catgtccgat
    10501 acagttacac aaaacagcgt cttgcggagc agactgtgtc tgaacttctg aaaaaaatcg
    10561 atgcgcgaac caagctcgcc ggcaccaaca agcttgagat cctgctgttc tcgctgggaa
    10621 cggaccaacg cactggtcgc cgcgagacgt ttggcatcaa tgtcttcaag gtgcgcgaag
    10681 tcatgcgtac cccggaaatc accgccgcgc cagaaatgac cgcgtcggtg gagggcatgg
    10741 tcagcctgcg cggccagctg gtgccggtga ttgacctggc caagtacacc gggatcgtca
    10801 ccgaaactaa acccgacatc atgatcgtga ccgagtacaa cggccacact caagggtttc
    10861 tggtcgaggc ggtggacacc atcctgcgcc tggactggtc gctgatgcgc gtgccgccgg
    10921 agatgatctc caaccgtatg ggcggcctgg ttactgcggt gaccgagctg aacgaccaga
    10981 cgctggtgat gatgattgac gtcgaaaagg tgctggccga aaccacccag atcgacgact
    11041 cttaccagtt cgtcaacatt gagccggtta gcgtgccgcg catggtgttc tacgccgacg
    11101 actcggcggt ggcgcgcaag cagattgagc gcacactcga cgccatgggc atccgccacg
    11161 ctggcgccat caatggccgc caggcttggg aagagctgca acgcctggcc acccaggccg
    11221 aaatggccca tcgtccgctc aacgaaatgc tgcatctggt gctgaccgat gtggaaatgc
    11281 cggagatgga cggctatatc ctcaccaaga tgatcaagac cgatatccgc tttgccggca
    11341 tcccggtgct gatgcattcc tcgctgtccg ggcattccaa ccagaagctc ggccagtcgg
    11401 tcggcgtgga cgagtatgtc tcgaagttcg agccgcacaa gctggcccag aaagtgcgcc
    11461 agctgctcaa gctggatgcg ccgcagtcca cgctgctgcc gtccggacag gccaccctgc
    11521 cgcgcgactg acccccgttc acctggctga aaataggttt agaagatgac cgcttccgac
    11581 gcttcgctgc ttgccaacat cgatgcccgc accaagctgg caggctccaa caaaatggag
    11641 atcctgctgt tcacgctggg cacccgcgaa atcttcggca tcaatgtgtt caaagtccgc
    11701 gaagtttcgc agactccgga aatcaccaag acgcccaata tgccgatggg tgtgcaaggg
    11761 gtgatctcgc tgcgtggcaa tatcattccg gtgattgcgc tggacaagtt catcggccgc
    11821 agcaagcctg acaatgtgta tgcctcgatg atcgtcaccg agttcaacaa aagcacccag
    11881 gcttttctgg tgggcgaagt agaccgcatc atccgcgtgg actgggacaa ggtgcgtgcg
    11941 ccggaaaaca tggtatccac cagccagcag gccatgatta ccgctgtgac cgaactggaa
    12001 gacggcaaac tggtgtcgat tctggacgtc gaacagattc tggccagtgt gattggtgaa
    12061 acccgcattc cggatatccc caaggccaat ctggatgcgg atcagttcat cttcttctgc
    12121 gacgattcgg tcgtggcacg caaggaaatc actggcatgc tggacaaaat gggcgtcaag
    12181 tatcatcagg ccaacaatgg ccgtgaagct tgggatcgcc tgcaagcctt cgccaaccgt
    12241 cagtggaacg agggcgagag tctgcacgac accctcaagc tgattctggt ggatgccgaa
    12301 atgccggaaa tggacggcta tgtactgacc cgcctgatca agaatgatcc acgctttgcc
    12361 gatatcccgg tggtgatgca ctcctcgctg tcgtccaacg ccaaccgggc aatgggcacc
    12421 agcgtgggcg tcgatgccta tgtggcaaaa ttcgaacccg cactgctggc agagaccatg
    12481 ctgccatttt tgcaacgata attccatgtg agctgattta
//
LOCUS       DAOPYX010000047        12517 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_5627_length_12517_cov_8.015808, whole genome shotgun sequence.
ACCESSION   DAOPYX010000047 DAOPYX010000000
VERSION     DAOPYX010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 12517)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12517)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12517
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5627_length_12517_cov_8.015808"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            4..79
                     /locus_tag="PKN55_03925"
     tRNA            4..79
                     /locus_tag="PKN55_03925"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:37..39,aa:Thr,seq:tgt)
     gene            complement(216..689)
                     /locus_tag="PKN55_03930"
     CDS             complement(216..689)
                     /locus_tag="PKN55_03930"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011134711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HNX70424.1"
                     /translation="MSAQDQTRFLSMTVLMTPDMANFSGNVHGGAILKLLDQVAYACA
                     SRYAGQYVVTLSVDQVTFKQPIHVGELVTFLAAVNHVGRTSMEIGIKVVAENIHSRVS
                     RHTNSCYFSMVAVDEQGKPVEVPRFEPANEEERARFTKAERRKALRRALAEENLH"
     gene            complement(827..1705)
                     /locus_tag="PKN55_03935"
     CDS             complement(827..1705)
                     /locus_tag="PKN55_03935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012698041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNX70425.1"
                     /translation="MSFPASWRAPALLLALWLIWGYNWVVSKTGLHWMGALEFAAGRT
                     VLATLTLGLILLGSRRALAPPPWRATLWLGLTQTTGFTGLTHLALVAGGAGKVSVLAY
                     TMPFWTLPLAWLWLGERVRGGQWLAIGLALLGLLAILEPWRASHTAGLPEVLALAGGV
                     SWAASAIIAKQLRASHQVDVLALTFWQMLYGLPPLLALAWLWPGAPTAWAEPGLWLTL
                     GFAGVFAGGIGWLMWLMLLGRLSAGAAGLNVLIIPAIAVLAAWLQLGEQPSSAECIGM
                     ALIALALTLLALRKSR"
     gene            complement(1743..2531)
                     /locus_tag="PKN55_03940"
     CDS             complement(1743..2531)
                     /locus_tag="PKN55_03940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012697673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HNX70426.1"
                     /translation="MYLNPILSTQNEHIKALARLAAKPAERRARGQLLLEGIHLLDAA
                     LQHGLAVDEVLVQSRALAHPEVSALLARLPASCRGHEVSEAVMAKLSSLNTPPELIAV
                     IPTPAAAAPNADDSLLLLDAVQDPGNLGAILRTAAAAGVRQVWLSPQCADPWSAKVLR
                     AGMGAHFAVALHTDAELTRVLAEFNGPAIVTSLDATQSLYALDLRQPCAFVFGNEGAG
                     VSAPVLARASHPVRIPMPGVAESLNVAAAVAVCLFEQVRQRLPG"
     gene            complement(2607..3947)
                     /locus_tag="PKN55_03945"
     CDS             complement(2607..3947)
                     /locus_tag="PKN55_03945"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HNX70427.1"
                     /translation="MTTWLDTLTQHRLPVLEATCKRVDAFRRIPSDQFNLADLSNVVL
                     ADPLMAFQVLRSTARIERTRTFSESLNIEHALMILGANAFFEEFNKFDALESMFSLSQ
                     TGVDQIISLMSRSRVAALLIKDWLSLMEEHRVEDCFVAALMHNVPACAFRAETGVDKS
                     FADLDVLARQAGFLDYKQMIERFIESQRLSDLLLDLLTTPPGFDRRRLLLKSAIEVAN
                     MLEQGWWRIELVEKIVGVAHLLARPFETIWHDMLQLVLNVARNPHVPGYGLLARQLAA
                     MPEPEQPLSQSFLNLSIQWDPASSSEPPAEFDQALNWVVSFLAKDMAQERVIFFRHNS
                     PHWQLSATQYVGLNDDDTLIARPLPLAQHPFFASLGQKAQAFRCQPGQLDRLRATFAD
                     DFFARIGDHGFAAMSVVRDGRLLGVFYFDNCSSGKPVDDLVYQQFKAAVSRLCA"
     gene            complement(3944..4669)
                     /locus_tag="PKN55_03950"
     CDS             complement(3944..4669)
                     /locus_tag="PKN55_03950"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNX70428.1"
                     /translation="MMRLHLRQLAIFLMALALAIVAGLLGAPWPLVWMQPPLAMLLAM
                     LWRTPRWWWLLHLLLLPAVFLAMQADLPPWLYLLAALLLFAISRNALTERVPLFLTSQ
                     AALDALAARLPPAARLIDLGCGTGRLLEGLSRRRPDLILHGVESAWLPWLWARLRLAR
                     RPNVSVTLGDIWPASLAGYGVIYTYLSPEPMARIWHKVKQEADSGAWLISNSFAVPDV
                     QPDESIAVDDATASTLYIWRLTT"
     gene            complement(4666..5631)
                     /gene="motB"
                     /locus_tag="PKN55_03955"
     CDS             complement(4666..5631)
                     /gene="motB"
                     /locus_tag="PKN55_03955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018747497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HNX70429.1"
                     /translation="MADESQRPIIIKRVKKGHGDHHGGAWKIAYADFVTAMMAFFLLL
                     WLLSSVSQGALNGIAEYFRTPLKIALMGGSRSGDTTSPIKGGGQDLTRTDGQLRKSHE
                     IFDEKSREKEKNRERDKQALSNLQKRIKEAIENNKILQQYKNQLLLEMTPEGLRVQIV
                     DEKNRPMFDSGSAQLLPHTRELLHELGKQLNEVPNRLTISGHTDASQYATGDRGMGNW
                     ELSSERANAARRELVGGGMQDNKLLRVIGQGSATPLDKANPLNPVNRRISIVVLNKES
                     EAAVLRESEPGETVYAPPGLANRMTPPAPATAATAATPPPGSPAQ"
     gene            complement(5657..6523)
                     /gene="motA"
                     /locus_tag="PKN55_03960"
     CDS             complement(5657..6523)
                     /gene="motA"
                     /locus_tag="PKN55_03960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008952786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HNX70430.1"
                     /translation="MFVIIGYIIVFSSVIGGFLLAGGHVAALIQPAEFIIIVGAAVGA
                     FITAHGGAPMKAIFAAVPGAFKASRYNKALYMELFALLYELLSKVRKEGLMSIEADVD
                     DPQNSPIFSKYPIVVDDHVVIEFLCDYLRLMVGGNLNPFEIENLMDIEIETHHSEGAM
                     PVNALARMADSLPAYGIVAAVMGVVHTMESVGVSPAELGRLIAAALVGTFLGILISYG
                     FVSPLSTRLEHRLNDVHRVLQCVKVTLLASLNGYAPPVAVEFGRKALNSEDRPGFLEL
                     EEHLKKARSGGR"
     gene            complement(6732..8111)
                     /locus_tag="PKN55_03965"
     CDS             complement(6732..8111)
                     /locus_tag="PKN55_03965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014087389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HNX70431.1"
                     /translation="MNTPRTTQAWQALDAAHHLHPFSDAHSLNQLGSRVVVRGEGVRI
                     WDSEGQCIIDGMAGLWCVNLGYGRKELAEAAYQQMLELPFYNTFFKTTHPPVIELSTL
                     LAELTPPQFNHVFLTNSGSESNDTVIRMARHFWAAQGQPQKKTLISRINAYHGSTIGG
                     ASLGGMSYMHAQGDLPIPGIVHIEQPYWYVNGGELSPEEYGLKAARALEAKILELGAE
                     NVAAFIGEPIQGAGGVIIPPSTYWPEIQRICREHDILLISDEVICGFGRTGRWFGCEY
                     FGFAPDLMPIAKGLSSGYQPIGGVMVGDRVAQVLMNSGDFNHGMTYSGHPVAAAVAAA
                     NLKILRDEGIVDRVRDDTGPYLQKKWQEAFADHPHLDDVRGVGFLAAFTWVKDKSRRE
                     LFANPGEVGTICRDIFFKNNLIMRAVGDSMVSAPPLVMTRTEIDEMIALAARCVDQTT
                     NTLRARGLI"
     gene            complement(8198..9532)
                     /locus_tag="PKN55_03970"
     CDS             complement(8198..9532)
                     /locus_tag="PKN55_03970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008952789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HNX70432.1"
                     /translation="MSSYMIEWMREHRITEVECIVPDMTGVARGKIVPKDKFISEPEM
                     RLPEAVLIQTVTGDYPDDSVLDPVDADMILQPDQNTIRFVPWAVDPTAQIIFDCYKSD
                     GQMSEAAPRAVLRRVLDLYAQRGWSPVIAPEMEFYLIATNPDPDIPVTPPVGRTGRAE
                     FGRRSYAIDAVNEFDPLFEDIYDYCHAQNLEVDTLIHEIGTAQMEINFLHGNPLDLAD
                     QVFLFKRTVREAAFRHGMYATFMAKPMEGEPGSAMHMHQSVIDTASGRNVFSNEDGSP
                     SPAFYHFIGGLQRYLPKTMPFFAPYVNSYRRLTPYTAAPTNVQWGFDNRTVGLRVPHS
                     SPAARRVENRVPGVDVNPYIAMAASLACGYLGMVNQIDCAEPVQINAYSLPYELPRGT
                     EDALEQLKACPEIAEILGPRFVELYIAMKEKEFSEYFRVISPWERKFLLLHV"
     gene            complement(9529..10320)
                     /locus_tag="PKN55_03975"
     CDS             complement(9529..10320)
                     /locus_tag="PKN55_03975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011135571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HNX70433.1"
                     /translation="MYAVVGIPCDIKPVGKHPFHAAGVKYVAAVAGGARCLPLLLPAL
                     GGQLPLAAALDQFDGVLLTGSLSNVEPQHYAGAASRPGTLHDPARDATTLPLIGEVIA
                     RGMPLLGICRGFQEINVALGGTLHQHVQEQAGLADHRDLSGDAPLEVSYGLAHPVRLR
                     PGSLLAQALGVDEIQVNSLHQQGVATLAPGLRAEAHAPDGLIEAFSLPDAPGWLHAVQ
                     WHPEWQYAHNPASLALFTAFGEACRQYRQARTGQPALPTPQGEAR"
     gene            complement(10549..10764)
                     /locus_tag="PKN55_03980"
     CDS             complement(10549..10764)
                     /locus_tag="PKN55_03980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX70434.1"
                     /translation="MQDGLRRLRSPLFTILDDLPIHATTMARMLHGKTGVSWHASLFA
                     ARIYKQSGGIVDFFKQTMRFASLTPMA"
     gene            10782..11327
                     /locus_tag="PKN55_03985"
     CDS             10782..11327
                     /locus_tag="PKN55_03985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008952792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNX70435.1"
                     /translation="MDVGARLRMVRDKFGLSQRELAKRAGVTNGTISLIEQNRVSPSI
                     SSLKKVLEGLPMSLADFFTFDAETAAQQVFYRHDELPDLGNNGIELRLVGVGHERRDI
                     AILRERYEIHADTGPDMLQHDGQEGGVVIRGHVELTVGTESRVLGPGDAYYFDSQQPH
                     RFRNVGDEICEVVSASSPPTF"
     gene            complement(11535..12095)
                     /locus_tag="PKN55_03990"
     CDS             complement(11535..12095)
                     /locus_tag="PKN55_03990"
                     /inference="COORDINATES: protein motif:HMM:NF024978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4136 domain-containing protein"
                     /protein_id="HNX70436.1"
                     /translation="MRSMIRHALLASAALTLLAGCAQLSVQTDYNPARPLPPLRTYQL
                     GEVRVYDAQGRLDADNTLQKDRIGRVLATAFAERGVSPLSRQADVQVNVEVEQREVSQ
                     IIDPRPRVSVGIGRGIYPGWGLGWQVPMQRDYQEGRLRVDLLKDGALLWRGETRATLN
                     SNGQWDEAGMLDGMRQVIKALPPPAP"
     gene            complement(12131..>12517)
                     /locus_tag="PKN55_03995"
     CDS             complement(12131..>12517)
                     /locus_tag="PKN55_03995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014087393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HNX70437.1"
                     /translation="GSDARVEQGDKVLTVFADDDFKLEQVIDVLKTKLSKRNVDIRCL
                     DYAKVEKVSGNKVKQAITVKVGVDTELAKKLVRLIKDSKLKVQASIQGDAVRVSGAKR
                     DLLQECIAMVRKEVTDFPLQYNNFRD"
BASE COUNT         2419 a         3900 c         3886 g         2312 t
ORIGIN      
        1 ttcgccggcg tagctcagtt ggtagagcag ctgacttgta atcagaaggt cgagggttcg
       61 actcctttcg ccggcaccag ttttttgcag caaatttaac gggttatcgt gttcacacac
      121 gaaacccgtt ttattttttg gcacatcttg tgccaaattt gtgccagatc accatgcctg
      181 atgatctggc gtgatgcctg ccgccgtcag tccgctcaat gcaaattctc ttccgccagc
      241 gcccggcgca atgccttgcg gcgctcggcc ttggtaaagc gcgcgcgttc ttcttcgttg
      301 gccggctcaa agcgcggcac ttccaccggt ttgccctgtt cgtccaccgc caccatgctg
      361 aaataacagc tattggtgtg ccggctgacg cgcgagtgga tgttttccgc caccaccttg
      421 atgccgattt ccatcgaggt gcggccgaca tggttcaccg ccgccagaaa ggtcaccagc
      481 tcgccgacgt ggatgggttg tttaaacgtc acctgatcca ccgacagggt caccacatac
      541 tggccagcgt aacggctggc gcaggcgtag gccacttggt cgagcagttt caggatggcg
      601 ccgccgtgca cattgccgga aaaattggcc atgtccggcg tcatcagcac ggtcatcgat
      661 aaaaagcggg tctggtcttg cgcggacatt gggtgaatct cctcgtgggg tggttttcat
      721 gtgtagaacg gctcataaaa ccctcctggc gtcgttgcgc ggccttgccg tacgcttgta
      781 ctgtctgcgg ccgcacgcct agccaggacc gctgcgctgg gttttgttag cgactcttac
      841 gcagggcaag caaagtcagc gccagggcaa tcagcgccat gccaatacat tcggccgagg
      901 acggctgctc gcccagttgg agccaggcag ccagcacggc gatggccggg atgatcagca
      961 cattcagccc ggcggctccg gctgacaggc ggcccagcag catcaaccac atcagccagc
     1021 caatgccgcc ggcaaacacc ccggcaaagc ccagcgtcag ccataagccc ggttcggccc
     1081 aggcggtggg cgcacccggc cacagccagg ccagcgccag cagcggtggc agaccgtaca
     1141 gcatttgcca gaaggtcagc gccagcacgt ccacctgatg gctggcgcgc aattgcttgg
     1201 cgatgatggc gctggccgcc cagctgacac cgccagccag cgccagcacc tcgggcaggc
     1261 cggcggtgtg gctggcgcgc cagggctcca gaatggccag caggcccagc agcgccaggc
     1321 cgatggccag ccactggccg ccgcgcacgc gctcgcccag ccatagccag gccagcggca
     1381 aggtccagaa cggcatggtg taggccagca ccgacacctt gcctgcgccg ccagccacca
     1441 gcgccagatg ggtgaggccg gtaaagccgg tggtttgcgt cagccccagc cacagcgtgg
     1501 cgcgccaggg cggcggagcc aacgcgcggc ggctgcccag cagaatcagc cccaaggtga
     1561 gggtggccag cacggtgcgg cccgcggcaa actcgagcgc gcccatccag tgcaggccgg
     1621 ttttggacac cacccagttg taaccccaga tcagccagag cgccaacaac agcgccggcg
     1681 cgcgccagga agcaggaaaa gacatgagca cggacaatgc aggaagataa aaaaaagctg
     1741 gttcagccgg gcagccgctg gcgcacttgc tcaaacaaac acaccgccac cgccgccgcc
     1801 acgttaaggg attccgccac acccggcatc gggatgcgca ccgggtggct ggcgcgggcc
     1861 agcactggcg ccgacacgcc ggcgccttcg ttgccaaaca caaaggcgca cggttggcgc
     1921 aaatccagcg catacagcga ttgcgtggcg tccaggctgg tgacaatggc cgggccgttg
     1981 aattcggcca gcacgcgggt gagctcggcg tcggtgtgca gcgcgacggc aaagtgcgcg
     2041 cccatgccgg cgcgcagcac cttggccgac cacggatcgg cgcactgcgg cgacagccac
     2101 acctgacgca cgccagcggc ggcggcggtg cgcagaatgg cccccaggtt gccggggtct
     2161 tgcaccgcgt ccagcagcaa caggctgtcg tcggcgttgg gcgcggcggc agccggggtg
     2221 ggaatcacgg caatcagctc gggcggggta ttcaggctgc tgagcttggc catcaccgcc
     2281 tcgctgactt catggccgcg acagctggcc ggcaggcgcg ccagcagcgc cgacacttcc
     2341 gggtgggcca gcgcgcggct ttgcaccagc acttcgtcca ccgccaggcc gtgctgcaaa
     2401 gcggcgtcca gcagatggat gccttccagc aataactggc cgcgcgcgcg ccgctcagcc
     2461 ggcttggccg ccagccgcgc cagcgccttg atgtgttcgt tttgggtaga cagaatcggg
     2521 ttcagataca ttgggcagcc ccagaaaaca gttcgcccgg ccagcaagcg ccagccgggc
     2581 ggaaatcgcc atcagatcaa gcagggtcag gcacacagcc gcgacacggc ggccttgaat
     2641 tgctgataaa ccagatcgtc caccggtttg ccagacgagc agttgtcaaa ataaaacacg
     2701 cccagcaagc gcccatcgcg caccaccgac atggcggcaa aaccatggtc gccaatgcgg
     2761 gcaaagaaat catcggcaaa ggtggcgcgc agccggtcca actggccggg ctggcagcgg
     2821 aaggcttgcg ccttttgccc caggctggca aagaacggat gctgggccag cggcagcggg
     2881 cgggcaatca aggtgtcgtc gtcgttcagg ccaacatact gggtggcaga cagctgccag
     2941 tgtgggctgt tgtggcggaa aaagatcacc cgctcttgcg ccatgtcctt ggccaggaag
     3001 ctgaccaccc agttcaatgc ctggtcgaat tcagccggcg gctcgctgct gctggccgga
     3061 tcccactgga tgctgaggtt cagaaagctc tggctgagcg gctgttccgg ctcaggcatg
     3121 gcggccagtt ggcgggccaa aaggccatag cccggcacat gcgggttgcg cgccacattg
     3181 agcaccagtt gcagcatgtc gtgccagatg gtttcaaacg gacgcgccag caggtgggcc
     3241 acgccgacga ttttttccac cagctcaatg cgccaccagc cttgttccag catattggcc
     3301 acttcgatgg cgcttttcag cagcaggcgg cggcggtcaa agccgggcgg cgtggtcagc
     3361 aggtcaagca gcagatcgga cagacgctgg ctttcgataa agcgctcgat catctgtttg
     3421 taatccagaa agcctgcctg ccgcgccagc acatccagat cggcaaagga tttgtccacg
     3481 ccggtttcag cacggaaggc gcatgccggc acattgtgca tcagtgcggc cacaaagcag
     3541 tcttccacgc ggtgttcttc catcagcgac agccaatcct tgatcagcag cgccgccacg
     3601 cgcgagcgcg acatcaggct gatgatctga tccaccccgg tttgcgacag ggagaacatg
     3661 gattccagcg cgtcgaattt gttgaattct tcaaaaaacg cattggcgcc cagaatcatc
     3721 agcgcgtgct caatattcag cgattcgctg aaggtgcgcg tgcgttcgat gcgcgcggtg
     3781 ctgcgcaaga cctgaaacgc catcagcggg tcggccagca ccacattgga caggtcagcc
     3841 aggttgaact ggtcgctggg aatgcggcga aaggcatcca cgcgtttgca ggttgcctct
     3901 agcaccggca gacggtgctg agtcagggta tcaagccagg tagtcatgtc gtcaaacgcc
     3961 aaatgtacag ggttgaggcg gttgcatcgt caaccgcaat cgattcgtcc ggttggacat
     4021 cgggcaccgc aaaagagttg ctgatcagcc aagcgccgga atcagcctct tgttttactt
     4081 tatgccagat tctggccatt ggttccgggg aaagataggt gtaaatcacc ccataaccgg
     4141 ccagtgaggc cggccagatg tcacccagcg tcacgctgac attgggccgg cgggccaagc
     4201 gcagccgcgc ccacagccag ggcagccagg cgctttccac cccgtgcaga atcaggtcgg
     4261 gccggcggcg tgacaggcct tccagcaggc ggccggtgcc acagcccaga tcgatcagtc
     4321 gggcagcggg gggcaggcgg gcggccaggg catccagcgc ggcctgtgaa gtcaggaaca
     4381 gcggcactcg ctcggtcagc gcattgcggc tgatggcaaa cagcagcaat gcagccagca
     4441 gatatagcca aggaggcaga tcagcctgca tcgccaaaaa gacagcgggc aacaacagca
     4501 gatgcaacag ccaccaccag cgtggtgtgc gccacagcat ggccagcagc atcgccagcg
     4561 gcggctgcat ccacaccagc ggccacgggg cgcccagcag gccggccaca atggccagcg
     4621 ccagcgccat cagaaagata gccagctggc gcaggtggag tcgcatcatt gagcgggcga
     4681 gcctggcggc ggggtagccg cagtagctgc agtggccgga gccggcggcg tcatccggtt
     4741 ggccaggccc ggcggggcgt aaaccgtttc acccggctcg ctttcccgca ggacagccgc
     4801 ttcggattct ttgttcagca ccacgatcga aatacggcgg ttaaccggat tgagcgggtt
     4861 ggccttgtcc agcggggtgg ccgagccctg accgatcacc cgcaacagct tgttatcctg
     4921 catgccgcca ccgaccagtt cacgacgggc agcattggcg cgctcggacg acagctccca
     4981 gttgcccatg ccacgatcgc cggtggcgta ttgcgaggca tcggtgtggc cggagatggt
     5041 cagccggttg ggcacttcgt tcagctgttt gcccagttca tgcagcagct cgcgggtatg
     5101 tggcagcaat tgtgcgctgc ccgagtcgaa catcggccgg tttttctcgt ccacaatctg
     5161 tacgcgcagg ccttccggcg tcatttccag caacagttga ttcttgtact gctgaagaat
     5221 cttgttgttt tcgatggcct ctttgatgcg tttttgcagg ttggacagcg cctgcttgtc
     5281 gcgctcacga tttttttctt tctcgcggct cttttcatca aagatttcgt gcgacttgcg
     5341 cagctgacca tcggtgcgag tgagatcctg cccgccgccc ttgatcgggc tggtggtgtc
     5401 gcccgagcgg ctgccgccca tcagcgcgat cttcagcggg gtgcgaaagt attcggcaat
     5461 gccgttgagc gcgccctgcg acaccgagga cagcagccac aggagcagga agaatgccat
     5521 catggccgtc acaaagtcgg cgtaggcaat cttccaggcg ccgccatggt ggtcgccatg
     5581 gcccttcttg acgcgtttga taataatagg gcgttgagat tcgtctgcca tgattcctgc
     5641 cttgtctcaa ccgcgatcaa cgaccgccgc tgcgagcttt cttgagatgt tcttccagct
     5701 ccaggaagcc cggacgatct tcgctgttga gcgccttgcg gccgaattcc accgccaccg
     5761 gcggggcgta gccgttcagg ctggccagca gggtgacttt cacgcattgc agcacgcggt
     5821 gcacgtcgtt caggcggtgt tccagacgag tggacagcgg gctgacgaaa ccgtacgaga
     5881 tcaaaatacc caggaaggtg cccaccaggg cggcggcaat cagccggccc agttcggcag
     5941 gcgacacgcc caccgattcc atggtgtgca ccacgcccat caccgccgcc acaataccgt
     6001 aggcgggcag cgagtcggcc atgcgcgcca gcgcgttcac cggcatggcg ccttcgctat
     6061 ggtgggtttc gatttcgatg tccatcaggt tttcgatttc gaacggattc aggttacccc
     6121 ccaccatcag gcgcaggtag tcacagagaa attcgatgac tacgtggtcg tcgaccacaa
     6181 tcgggtactt ggaaaaaatc ggactgttct gcgggtcgtc gacatcggct tcgatcgaca
     6241 tcaggccttc tttacgcacc ttggacagca gctcgtacag cagggcaaac agctccatgt
     6301 acagcgcctt gttgtagcgc gaggccttga acgcaccggg gacggcggca aagatggcct
     6361 tcatcggcgc gccgccgtgg gcagtgatga aggcacccac cgctgcaccc acgatgatga
     6421 tgaattccgc tggctggata agtgcagcca cgtgaccgcc ggccagaagg aagccgccga
     6481 taacgctgga aaaaacgatg atgtagccga tgattacaaa catgtaccga gatccctggg
     6541 tttttgttat ctatcgtctg cgcctgtcct ggcttaaggc cgggcgccac aaacagaaaa
     6601 gcggctcgat tctatcaaac ccgcaggccg tgcgcgaaaa cttttatcct tattggattg
     6661 cagcttgcgc tcgcgtcccc atcaagcatg ttcgcgccag atggtggacg cgccgccctg
     6721 ggggggcgaa gtcaaatcag gccgcgtgcg cggagggtgt tcgtggtctg atccacacaa
     6781 cgcgcagcca gtgcaatcat ctcatcaatt tctgtccggg tcattaccag cggcggcgcg
     6841 ctgaccatgc tgtcgcccac ggcgcgcatg atcaggttgt tcttgaaaaa gatgtcgcgg
     6901 cagattgtgc cgacttcccc ggggttggcg aacaactcgc gccgtgattt gtccttgacc
     6961 caggtaaacg ctgctaaaaa gcccacgccg cgcacatcgt ccagatgtgg gtggtcggca
     7021 aatgcttctt gccatttttt ttgcaaataa gggccggtgt cgtcgcgcac ccggtcgacg
     7081 atgccttcgt cgcgcaggat tttcaggttg gccgccgcca ctgccgccgc caccgggtgg
     7141 ccggagtagg tcatgccgtg gttgaaatcg cccgaattca tcaacacctg ggccacccgg
     7201 tcgccgacca tcaccccgcc aatcggctga taacccgacg acaagccctt ggcaatcggc
     7261 atcagatccg gcgcaaagcc aaaatactca cagccaaacc agcgcccggt gcggccaaag
     7321 ccgcaaatca cttcgtcgct gatcagcaaa atatcgtgct cgcggcagat gcgctggatt
     7381 tccggccaat aggtggatgg cggaataatc accccgccgg cgccctgaat cggctcgcca
     7441 ataaaggcgg cgacgttttc ggcgcccagt tcaaggattt tggcttccag cgcgcgggcg
     7501 gctttcaggc cgtattcttc cgggctgagt tcgccgccat tcacatacca atacggctgc
     7561 tcgatatgca caatgccggg aatcggcaaa tcgccctgcg cgtgcatata gctcatgccg
     7621 cccagcgagg cgccgccaat ggtggagccg tgataggcgt tgatgcggct gatcagggtt
     7681 tttttctgcg gctggccttg cgccgcccaa aaatgccgcg ccatgcggat cacggtgtcg
     7741 ttggattccg agccggaatt ggtcaaaaac acatggttga actgcggcgg ggtcagctcg
     7801 gccagcaggg tggacagctc gatcaccggc gggtgagtgg tcttgaaaaa ggtattgtaa
     7861 aacggcagtt ccagcatttg ctggtaagcc gcctcggcca actccttgcg gccatagccg
     7921 agattgacgc accacagccc ggccatgccg tcgatgatgc actggccttc cgaatcccag
     7981 atgcgcacgc cttcgccgcg caccaccacc cggctgccca attgattgag cgaatgggcg
     8041 tcagagaacg gatgcagatg atgggcggcg tcaagcgcct gccacgcttg cgtggtgcgg
     8101 ggggtgttca tgatgcggct cctggtgacg ttggccatgc gcccatggca tggccaacgg
     8161 aataatctgg gagaggatgg gatgaatggc gtgcagttta cacatgcagc agcaggaatt
     8221 tacgctccca cgggctgatc acgcggaaat actcagagaa ttctttttct ttcatggcga
     8281 tgtacagctc gacaaagcgc gggccaagaa tctcggcgat ttccgggcag gccttgagtt
     8341 gctccagggc atcttcagtg ccgcgcggca gctcgtaagg cagcgaatag gcgttgatct
     8401 gcaccggctc ggcgcagtcg atctggttga ccatgcccag atagccgcag gccagcgagg
     8461 cggccatggc gatataggga ttgacgtcca cccccggcac gcggttttcc acccggcgcg
     8521 ccgccgggct ggagtgcggc acgcgcaggc ccaccgtgcg gttgtcaaag ccccattgca
     8581 cattggtcgg cgcggcggtg tacggggtca gccggcggta agagttcaca tatggggcaa
     8641 aaaacggcat ggttttcggc agatagcgct gcaagccgcc gatgaagtga taaaacgccg
     8701 gcgacgggct gccgtcttcg ttggagaaca cattgcgccc gctggcggtg tcgatcacgc
     8761 tctggtgcat atgcatggcg ctgcccggct cgccttccat cggcttggcc atgaaagtcg
     8821 cgtacatgcc atggcggaac gccgcctcgc gcaccgtgcg cttgaacaaa aacacctggt
     8881 cggccaggtc cagcgggttg ccgtgcaaga agttgatttc catctgcgcg gtgccgattt
     8941 cgtgaatcag cgtgtccact tccaggtttt gcgcatggca gtagtcgtag atgtcctcga
     9001 acagcgggtc aaattcgttc acggcatcaa tggcgtagga gcggcggcca aattcggcgc
     9061 gaccggtgcg ccccactggc ggcgtcaccg ggatgtccgg gtccgggttg gtggcgatca
     9121 ggtaaaactc catttccggg gcaatcaccg gcgaccagcc gcgctgggcg tataaatcca
     9181 gcacccggcg cagcacggcg cgcggcgcgg cttccgacat ctggccatcg gacttgtagc
     9241 agtcaaagat gatctgcgcg gtcgggtcca ccgcccaggg cacaaagcgg atggtgtttt
     9301 gatccggttg cagaatcatg tcggcgtcca ccgggtccag cacgctgtcg tccgggtaat
     9361 cgccagtcac agtttgaatc agcacggctt ctggcagccg catttctggt tcgctgatga
     9421 acttgtcctt gggcacaatc ttgccgcgcg ccacgccggt catatcgggg acaatgcatt
     9481 ccacttcggt gatgcgatgc tcgcgcatcc attcgatcat atagctgctc atcgggcttc
     9541 tccttgcggg gtgggcaacg ctggctgacc ggtgcgggcc tgccggtatt gccgacaggc
     9601 ttcgccaaac gcggtaaaca gcgccagcga agccgggttg tgtgcatact gccattccgg
     9661 gtgccattgc accgcgtgca gccagcccgg cgcatcgggc aggctgaagg cttcgatcag
     9721 cccatccggc gcgtgcgcct cggcgcgcag gccgggggcc agggtggcca cgccttgctg
     9781 atgcagtgag ttcacctgaa tctcgtccac gcccagcgcc tgggccagca ggctgccggg
     9841 gcgcagccgc accgggtgcg ccagcccgta gctgacctcc agcggcgcgt cgccggataa
     9901 atcccggtgg tcggccaggc cggcttgttc ctgcacatgc tgatgcagcg taccgcccaa
     9961 ggcgacgttg atctcttgaa agccgcggca aatgcccagc aagggcatgc cgcgcgcgat
    10021 cacttcgcca atcagcggca gcgtggtggc gtcgcgcgcc gggtcgtgca gcgtgccggg
    10081 gcggctggct gcgccagcgt agtgctgcgg ctcgacattg gacaagctgc cagtcagcaa
    10141 cacgccgtca aactgatcca gcgccgccgc cagcggcagt tgcccgccca gcgccggcag
    10201 cagcagtggc aaacaacggg cgccgccggc cacggcggcg acgtatttca cgccagcagc
    10261 atgaaacgga tgcttgccta ccggcttgat gtcacaggga attcccacta cggcgtacat
    10321 agcgcgctcc ttgcacccat ccggaagaag aacagcccac ccaaccctcc tggcgttgtt
    10381 gcccgatctt gccgtgctgt cagcacggtc tgcggtcgcg cgcctggcca ggcgcgctgc
    10441 gctgagtttt gtgagctgtc cgaaatatct gtcaggactg atgccatcgt taacaagttc
    10501 ttaagttctg cggccatagc gattaccata ggcggccgcg ccgccggctc aggccatggg
    10561 cgtgagtgag gcaaatcgca tggtttgttt aaaaaaatct acaataccgc cagattgttt
    10621 gtaaattctg gctgcaaaca ggctggcatg ccaagaaaca ccggttttgc catgcagcat
    10681 tcgtgccatt gtcgtagcat gaatgggcag gtcgtctaga attgtgaaca gtggtgaccg
    10741 caagcggcgc aggccgtctt gcatatcagg aaggatcagg catggatgtg ggcgcgcgtt
    10801 tgcgcatggt gagggacaag ttcgggctgt cgcagcgtga gctggccaaa cgggccggcg
    10861 tcaccaatgg caccatttcc ctgattgaac agaaccgggt cagcccgtcg atcagctcgc
    10921 tgaagaaagt gctggaaggt ctgcccatgt cgctggccga cttcttcacc ttcgacgccg
    10981 agacggccgc acagcaggtg ttttaccgcc atgatgagct gcccgacctg ggcaataacg
    11041 gcattgagct gcggctggtg ggcgtcgggc acgagcggcg cgacatcgcc atcctgcgcg
    11101 agcgctatga gattcacgcc gacaccgggc cggacatgct gcaacacgac ggccaggaag
    11161 gcggcgtggt catccgtggc catgtagagc tcaccgtagg cacagaatca cgcgtgctgg
    11221 ggccgggcga cgcgtattat tttgacagcc agcagccgca ccgctttcgc aatgtcggcg
    11281 atgaaatctg cgaagtggtc agcgccagct cgccgcccac cttctgatga tcccgtctga
    11341 cgggctcact gtcagccgcg ccgattgcgt ggcgtttggt gagccgcgta gcaaacaggg
    11401 ggcgccaggc agccacactt agagcggcta acaaacccct cctggcgttg ttgcccgacc
    11461 ttgccgtact accccgtact gtctgcggtc gcgcgcctgg ccaggatcgc ttcgctcagc
    11521 tttgttagcc acacttaggg tgctggcggc ggcagcgcct tgatcacctg acgcatgcca
    11581 tccagcatac cagcctcgtc ccactgacca ttgctgttga gcgtggcgcg ggtttcgccg
    11641 cgccacagca gtgcaccatc cttgagcagg tccacccgca agcggccttc ctgatagtcg
    11701 cgctgcatcg gcacttgcca gcccaaaccc cagcccggat agatgccccg gccaatgccc
    11761 acgctgacgc gcgggcgtgg gtcgatgatc tggctgactt cgcgctgttc gacttccaca
    11821 ttcacctgca catcggcctg acgcgacaag gggctgacac cgcgctcggc aaatgccgtg
    11881 gccagcacac ggccgatgcg gtctttttgc aaggtgttgt cggcgtccag ccggccctgg
    11941 gcgtcgtaaa cgcgcacctc gcccagttga taagtacgca gcggcggcag cggtcgggcc
    12001 gggttgtagt cggtctgcac cgacaactgg gcgcagccag ccagcagcgt cagcgcagcg
    12061 ctggccagta aagcatggcg aatcatggag cgcatcgcac cctccagaac ccggcggcgc
    12121 gccgggagta tcaatcgcgg aagttattgt attgcagcgg aaaatccgtg acttccttgc
    12181 gcaccatggc gatgcactct tgcagcaaat cgcgcttggc gccggacacg cgcacggcgt
    12241 cgccctgaat cgacgcctgc accttcagct tgctgtcctt gatcagacgc accagctttt
    12301 tggccagctc ggtgtccacg cccactttga cggtgatggc ctgcttgacc ttgttgccgc
    12361 tgactttttc gactttggcg taatccaggc agcggatgtc cacattgcgc ttgctgagct
    12421 tggtcttgag cacgtcaatc acctgctcca gcttgaaatc atcgtcggca aacacggtca
    12481 gcaccttgtc gccctgctcg acgcgggcgt ccgagcc
//
LOCUS       DAOPYX010000048        12513 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_5629_length_12513_cov_9.062209, whole genome shotgun sequence.
ACCESSION   DAOPYX010000048 DAOPYX010000000
VERSION     DAOPYX010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 12513)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12513)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12513
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5629_length_12513_cov_9.062209"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..797)
                     /gene="prmC"
                     /locus_tag="PKN55_04000"
     CDS             complement(<1..797)
                     /gene="prmC"
                     /locus_tag="PKN55_04000"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019389826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HNX70438.1"
                     /translation="MSTLAQLLADAGLPRLEAQLLLHMAGGGNRVTQLTWPEREASAE
                     VAQRFAALCARRHAGEPIAYLLGEREFFGLDLAVSPAVLIPRPDTEILVDTALARLPA
                     DGRALDLGTGSGAVALALAHSRPDADIWALDASPEALAQAQANGQRLNLAVTWRLSDW
                     YAAVAGEQFDLLVSNPPYIAADDPHLQQGDLRFEPAGALTDFADGLSCIRQLIAGAPT
                     HLRPGGWLLMEHGWQQAPAVRELLSAAGFIDVASWQDLAGHERVTGGR"
     gene            complement(794..2236)
                     /gene="mgtE"
                     /locus_tag="PKN55_04005"
     CDS             complement(794..2236)
                     /gene="mgtE"
                     /locus_tag="PKN55_04005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008953273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HNX70439.1"
                     /translation="MNTLDTQKTQDSLQGSLQQVLALLHRHRLVENLVHKQDMPKHTL
                     VESLVHKQNLRALQKKLDQLHTADVAYILESLPLEDRLVVWELVKAERDGDILLEVSD
                     AVRESLIEVMDTHELVAAAEQLDADELADLAPDLPQQVVYEVMDGLDSKEREQLQSAL
                     SYTEDQVGSLMDFELVKIRADVTLEVVLRYLRRFEELPDQTDKIFVVDEDNRVQGVLP
                     LKTLLVSDPDALVEVVMARDVVVFHPEDDSGDAAQAFERYDLITAPVVNADNQLIGRL
                     TVDEMVDVIREESDSEVLSMAGLKEEEDLFASVWDSVKNRWAWLAINLVTAFVASRVI
                     GVFEGAIEQLVALAALMPIVAGIGGNSGNQTTTMIVRALALGQLQTSSAMRLWRKELG
                     VSMVNGLVWGGIIGLIASLLYGSFSLGLVMTAAMTLNLMLAATIGVMIPMLMVRAGRD
                     PAMGSSVMITACTDSGGFFIFLGLATLFLL"
     gene            complement(2467..>2847)
                     /locus_tag="PKN55_04010"
     CDS             complement(2467..>2847)
                     /locus_tag="PKN55_04010"
                     /inference="COORDINATES: protein motif:HMM:NF018672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HNX70440.1"
                     /translation="NTGDNTMWMTIATALTALIAVLHGYFMWLEMCGWTRPAGRRAFG
                     LTIEFAEQTRRLAANQGLYNGFLAAGLAWSLWPGSPAELPWFFLACVLVAGVYGGYSV
                     SRKIFWIQGAPAALALVALALARA"
     assembly_gap    2848..2947
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2948..3409)
                     /locus_tag="PKN55_04015"
     CDS             complement(2948..3409)
                     /locus_tag="PKN55_04015"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HNX70441.1"
                     /translation="MSPAIFTQTVEVRWSDLDANQHMRHSAYADLCTHARLGWLAQSG
                     FAGGEFARLGLGPVLFQESTEYFREVHLGEVLTIDIRLEAASPDYGRWKLRQDMHKAD
                     GRLAATHRVAGAWLDLTTRKLIAPPTVLAEIFAALTQVDFTPLPQPQYRSA"
     gene            3476..4090
                     /locus_tag="PKN55_04020"
     CDS             3476..4090
                     /locus_tag="PKN55_04020"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HNX70442.1"
                     /translation="MTAKKMQTREHILRQALALASQVGVAGLTIGSVAEACGLSKSGM
                     FAHFGSKEALQLAVVENAQQHFVREVFQPILTLERGLPRLQALMAAWLGRLDAGHDLP
                     GGCPLMAAVYEFDDQPGAVRDALVAGQGWQRDTLLRLVQSACEAGQLPADTDAEVLAL
                     LLFGLVQGVHHDARLLGRADSATLARRACAILLAQPPRLSTMPR"
     gene            4220..5494
                     /gene="serS"
                     /locus_tag="PKN55_04025"
     CDS             4220..5494
                     /gene="serS"
                     /locus_tag="PKN55_04025"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009118021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HNX70443.1"
                     /translation="MLDIQLLRNELDAVAARLAARGFTLDAEAFAQLEHERKTLQTRT
                     QDLQARRNSLSKQVGEAKRKGEDASAILAEVAGLGDELKANETALAQLQDRLSALLMT
                     IPNLPHESVPAGRDESDNVEVRRVGEPRAFDFPVKDHVDLGAALGLDFDTAAKLSGSR
                     FAVLKGQMARLHRALAQFMLDTHVSEHGYTEVYVPYMVNADSMRGTGQLPKFEEDLFR
                     IPRGDDTFYLVPTAEVPVTNFMRDELVKAEQLPMRYCAHTPCFRSEAGSYGRDTRGMI
                     RQHQFDKVEMVQIVRPEESFAALEALTGHAEAILQKLGLPYRTIVLCTGDMGFGACKT
                     YDLEVWLPAQNTYREISSCSNMGDFQARRMQARFKNEQGKNELVHTLNGSGLAVGRTL
                     VAVLENYQNADGSVTVPEALRPYMGGLDTLRP"
     assembly_gap    5591..5690
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(5694..6539)
                     /gene="asd"
                     /locus_tag="PKN55_04030"
     CDS             complement(5694..6539)
                     /gene="asd"
                     /locus_tag="PKN55_04030"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011134146.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HNX70444.1"
                     /translation="MSDRLAVLPQYLLPKQALTALAGKVAQAQAGGLTTQLIRWFVQR
                     YQVDMHEAANPDIASYPSFNAFFTRPLKAGARPLAQTALVCPVDGAISQCGPIAGEQI
                     FQAKGHHYSATALVGGDAALAAQFQDGAFATLYLSPRDYHRIHMPCAGRLTRMIYVPG
                     ALFSVNPTTARGVPGLFARNERVVCVFESEYGPFVLTLVGATIVGSMATTWHGVINPP
                     RPGLVREWRYDGADAPQLAKGEEMGRFLLGSTVVMLFPKGMLDFNPDWVPARPVRMGE
                     AMSAR"
     gene            complement(6688..6778)
                     /locus_tag="PKN55_04035"
     tRNA            complement(6688..6778)
                     /locus_tag="PKN55_04035"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6742..6744),aa:Ser,seq:gga)
     gene            7283..7702
                     /locus_tag="PKN55_04040"
     CDS             7283..7702
                     /locus_tag="PKN55_04040"
                     /inference="COORDINATES: protein motif:HMM:NF018110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlhE"
                     /protein_id="HNX70445.1"
                     /translation="MKKAILICAMLGFAAQSHAVVGSWSKQTAHYPIYFSGGYAEMPF
                     NAPASMPTLTSFVTSVSWQWQSYGNGSSSEIVLICYSKKYSNVIGNCNRINAQQGSTS
                     VFNGFDASGKFWIKHTLTGGNYPAISNGFDQVTVNHQ"
     gene            7859..8944
                     /locus_tag="PKN55_04045"
     CDS             7859..8944
                     /locus_tag="PKN55_04045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX70446.1"
                     /translation="MNNISSLGASNSAYQAKNGAIASAASADASAKNQPSSSAQVTLS
                     GKAVMLSRLWRINDPSASAPKVLSSVTTNMLTGNSYDFLSSSDRKFLEKVYAYAQEKN
                     IDLAQVDNLATDMAAYRMLYATNGPGLSAIGPGNIFTTDGKEIVYAFNESDYYTAERI
                     KSDSALSSTEVDGGFLRYALDPWQNSVHAVDFKFLERVVIDFSAETGRSINENTVTAG
                     KGSSDLHFGVNGYYVKEKSSTSSSTSDKATNNTPNVLSYLSEQDRKQLIGAYKMLMSL
                     GMSTEQLDQAATRLGSMRMHTVTGQSSASSDASAQTAKAIGAYHRSDSAFSLFDGMLP
                     ANKPKNHTPIVQMSSVLEKLLMAEKAE"
     gene            9028..10095
                     /locus_tag="PKN55_04050"
     CDS             9028..10095
                     /locus_tag="PKN55_04050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX70447.1"
                     /translation="MSISAIISPTSSEVQLSKSSAASTSAQESRVSRGHTLMLERLWR
                     IKDPSAPEPKILSTVTTDMLSGNSFDFLSYSDRRLLEKAYTYAEDNNIDLEQVDLLAF
                     DMAGYRMAGPGGHIPNKPGNSYTLDGKWIINAFDERDTKIAYQIKNGKAMLSTEFDRG
                     FLNEALDPEKAPTHGVDFAFLAKMVKEFSDQAGQPDENLQMPDDPNRRYAEPGRGSYL
                     VETLNGKKPPTHPDNLGKNADGSGDVLNYLTKQDRKKLLQAYQMLMSLGMSTDQLDQT
                     ATSLGAVRMREATGQSAPASSTSATHKATRAYSSQNGLTSLLDTLSPANRQKNRQPVT
                     QMSKVLEKLLMAEKAEQAGRA"
     gene            complement(10127..10897)
                     /locus_tag="PKN55_04055"
     CDS             complement(10127..10897)
                     /locus_tag="PKN55_04055"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HNX70448.1"
                     /translation="MMAHWGMLGTALAMAGLGAALGGIGGLLGIGGGLLAIPVLALWF
                     GMDQSMAQGTALVMIVPNVLLAFYRYRQHHTIPLSRALGLGGIAMLASYPAARLAVGL
                     DHHTLQWVFIGFLLGLCAYLCWLVARPPLAHAPQPRVLSARYLPVVGVVSGVFSGLFT
                     VGGGLVATPMLVRGFGHRQAAAQGLALALVVPGSLIALSAYASAGQVSWSLGLPMALG
                     GLSTISWGVACARRLPERWLRSVFIGFLLVTTALMWPV"
     gene            11002..11910
                     /locus_tag="PKN55_04060"
     CDS             11002..11910
                     /locus_tag="PKN55_04060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008952653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HNX70449.1"
                     /translation="MDGWSDLAFFAALLRAGSLTALAREQGVTPPVISRRLAALEARL
                     GMRLLQRTTRRMHLTQEGELYAQRGQVLLAELEALEADVSARHQTVSGLLRVNASFGF
                     GRRHIAPAVAAFSRQHPRVSVQLTLTDRPLNLVDSGFDLGIRVGGLPDSRDHACLLAG
                     NRRLLCAAPSYLARNPAPERPEALSRHQCIVIRENNQAYGNWQLSRAGQQVTVKVTGG
                     LSTNDGSTALAWGLAGHGILLRSTWEIAPHLRVGELIEVLPDWRFAPADVYAVYPQRA
                     QAPAKLSRFVAFLQAHFAAHRDGLAW"
     gene            complement(11917..>12513)
                     /locus_tag="PKN55_04065"
     CDS             complement(11917..>12513)
                     /locus_tag="PKN55_04065"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HNX70450.1"
                     /translation="APNVVLAFWRYSQHHPIALRRALTLGAVSIVVSYPAARLAVSLD
                     HHTLRWIFIGFLLWLVGYLGWQLYARRAKGAPAPRILSERWLPLVSVVAGAFSGLFTV
                     GGGTISSPMLVGLFGYRQAAAQGLGLCMIVPSSLVAMGAYTHAGVVDWQLGIPLAIGG
                     LSTISRGVALAQRLPDRKLRGIFLVFLVITVVLMLPKG"
BASE COUNT         2647 a         3765 c         3644 g         2257 t
ORIGIN      
        1 cgcccgcccg tcacccgctc gtgcccggcc aggtcctgcc aggaagccac gtcgataaac
       61 cctgctgcgc tgagcagctc acgcacggcc ggcgcttgct gccagccgtg ttccatcaac
      121 agccagccgc cggggcgcag gtgggtgggg gcgccggcga tcagttggcg gatgcagctc
      181 aggccgtcgg caaagtcggt cagcgcgccg gccggttcga agcgcaggtc gccttgttgc
      241 aggtgggggt cgtcggcggc gatatagggc gggttggaca ccaagagatc aaactgttcg
      301 cccgccacgg cggcgtacca gtctgacagc cgccaggtga ccgccaggtt cagccgctgg
      361 ccattggcct gcgcctgcgc cagcgcctcg ggcgaggcgt ccagcgccca gatgtcggcg
      421 tcggggcggc tgtgcgccag cgccagcgcc actgcgccgc tgccggtgcc cagatccagc
      481 gcgcggccgt cggccggcag ccgcgccagc gcggtgtcca ccaggatttc cgtgtccggg
      541 cgcggaatca gcacggccgg cgacacggcc agatccaggc caaaaaactc gcgctcaccc
      601 aatagatagg caatcggctc gcccgcgtgg cggcgggcgc acagcgcggc aaagcgctgg
      661 gccacctcgg cgctggcttc gcgttcgggc caggtcagtt gggtgacgcg gttgccgcca
      721 ccggccatgt gcagcaacag ttgcgcctcc aggcgcggca ggccggcatc ggccagcagt
      781 tgggcaaggg tgctcatagc aggaacaagg tcgccaggcc aagaaagata aagaacccgc
      841 cagaatcagt gcaggcggtg atcatcaccg acgagcccat ggccgggtcg cggccagcgc
      901 gcaccatcag catgggaatc atcacgccaa tggtggcggc cagcatcaga ttcagcgtca
      961 tcgccgccgt catcaccagc cccagcgaaa agctgccgta caagagcgag gcaatcaggc
     1021 cgatgatgcc gccccacacc aggccgttga ccatcgacac ccccagctcc ttgcgccaca
     1081 gccgcatggc gctgctggtt tgcaattggc ccagggccaa ggcgcgcacg atcatggtgg
     1141 tggtctggtt gccggagttg ccgccaatgc cggcgacaat cggcatcagc gcggccagcg
     1201 ccaccagctg ctcgatggcg ccttcaaaga cgccaatcac ccggctggcg acaaaagccg
     1261 tcaccagatt gatggccagc caggcccaac ggtttttcac cgaatcccac acgctggcga
     1321 acaggtcttc ttcttccttc agaccggcca tggacagcac ttcgctgtcg gattcctcgc
     1381 ggatcacgtc caccatctcg tccacggtca gccggccaat cagctgattg tcggcgttga
     1441 ccactggcgc ggtgatcaaa tcgtagcgct cgaaggcctg ggcggcgtcg ccgctgtcgt
     1501 cttcggggtg gaacaccacc acgtcacgcg ccatcaccac ttccaccagc gcgtccgggt
     1561 cggacaccag cagggttttc agcggcagca cgccttgcac gcggttgtct tcgtccacca
     1621 caaaaatctt gtcggtctgg tcgggcagct cttcaaagcg gcgcaggtag cgcaacacca
     1681 cttccagggt gacgtcggcg cgaatcttga ccagctcgaa gtccatcagg ctgccgactt
     1741 gatcttcggt gtaggacagc gccgattgca gctgctcgcg ctctttgctg tccaggccgt
     1801 ccatcacctc gtacaccacc tgctgcggca ggtcgggcgc caggtcggcc agttcgtcgg
     1861 cgtccagctg ttcggcggcg gccaccagct cgtgggtgtc catcacctcg atcagcgatt
     1921 cgcgcaccgc gtcggagact tccagcagga tgtcgccgtc gcgctcggcc ttgaccagct
     1981 cccacaccac cagccggtct tccagcggca gggattccag gatataggcc acgtcggcgg
     2041 tgtgcagttg gtcgagcttt ttttgcaggg cgcgcaggtt ctgcttgtgc accagcgatt
     2101 ccaccagggt atgcttgggc atatcctgtt tgtggacgag gttttccacc agacggtggc
     2161 gatgcagcaa agccagcacc tgctggaggc tgccttgcag actgtcctgc gttttttgag
     2221 tatcgagcgt gttcatgcgc gcatcctctc cccggcccct ggcgtcacgg gcatggtgag
     2281 aatgcggtga ggcgggcggt cagcggggaa gtgctgttat ggcgtttagt aagcggtaac
     2341 tgggtaagtc catggtcgta actgggcgaa gcccgagcat ttcaacggaa ggcgtaaata
     2401 taccactggc gggctaggcc tgccagtggc cggatgttgc agtgcaatga atcttgcttg
     2461 atgtcctcag gctcgcgcca gggccagcgc aaccagcgcc aatgcggctg gcgcgccctg
     2521 aatccagaaa atcttgcggc tgacgctgta acccccatac acacccgcca ccagcacgca
     2581 agccagaaaa aaccacggca attccgccgg gctgcccggc cacagcgacc acgccagccc
     2641 cgctgccaaa aacccgttgt acagcccctg attggcggcc agccggcggg tttgttcagc
     2701 aaactcaatg gtcaggccaa acgcgcgccg gcctgccggg cgcgtccatc cacacatttc
     2761 cagccacata aaatagccgt gcagcacggc aatcaaggcg gtcagggcgg tggcgattgt
     2821 catccacatg gtgttgtctc cagtgttnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2941 nnnnnnntca ggcgctacgg tattgcggtt gcggcagcgg ggtgaaatcc acctgtgtca
     3001 gcgcggcaaa aatctcagcc agcacggtgg gcggcgcaat gagtttgcgg gtggtcaaat
     3061 ccagccaggc gcccgccacg cgatgggtgg cagccagacg gccgtcggcc ttgtgcatat
     3121 cctggcgcag cttccagcgg ccatagtccg ggctggcggc ttccaggcga atgtcgatgg
     3181 tcagcacttc gcccagatgc acttcacgaa aatactcggt gctttcctga aacagcaccg
     3241 gccccaggcc caggcgggcg aattcgccac cggcaaagcc ggattgcgcc agccagccca
     3301 gccgggcgtg ggtgcatagg tcagcatagg cgctgtggcg catgtgctgg ttggcgtcca
     3361 gatcagacca gcgcacctcg acggtttggg tgaaaatagc cggggacatg attcctccaa
     3421 taaaaaagca cgaacggtcg ttctttttta acgtaaaatc gagcggatga ccagtatgac
     3481 agccaaaaaa atgcaaaccc gtgaacacat cctgcgccag gcgctggcgc tggccagcca
     3541 ggtgggggtg gccgggctga ccattggcag cgtggccgag gcctgtggtt tatccaaaag
     3601 cggcatgttt gcccactttg gcagcaaaga agcgctgcaa ttggcggtgg tggaaaacgc
     3661 tcagcagcac tttgtacgcg aggtgtttca gcccattctg accctggagc gcggcttgcc
     3721 acgcttgcag gccttgatgg ccgcctggct ggggcggctg gacgccggcc acgacttgcc
     3781 cggcggctgc ccactgatgg cggcggtgta tgaatttgac gaccagcccg gcgcggtgcg
     3841 cgatgcactg gtggccggcc agggctggca gcgcgacacg ctattgcgtc tggtgcaaag
     3901 cgcctgcgaa gccgggcagc tgccggccga caccgacgcc gaagtgctgg cgctattgct
     3961 gtttggcctg gtgcagggcg tgcaccacga cgcacgccta ttgggccgcg ccgacagcgc
     4021 cacgctggcc cgccgcgcct gcgccatctt gctggcgcaa ccgccccgcc tgagcaccat
     4081 gccaaggtga cacctgccac ctgccacctg gcccggcctg agcccaccgc gcccggcgca
     4141 tacaagcggt ataatggtcg gcaagacgcg ccacgcgcgc gcaacgcaca cgccaacatc
     4201 tcgatatcag gattttttca tgcttgacat tcaactgctt cgcaacgagc tggacgccgt
     4261 ggccgcccgc ctggccgccc gtggcttcac cctggacgcc gaggcgtttg cccagctgga
     4321 gcacgagcgc aaaaccctgc aaacccgcac gcaagacctg caagcccgcc gcaacagcct
     4381 gtccaaacaa gtgggcgaag ccaagcgcaa gggcgaagac gcctcggcga ttctggccga
     4441 agtggccggc ctgggcgacg agctgaaagc caacgaaacc gcgctggcgc agctgcaaga
     4501 ccgcctgtcg gccctgctga tgaccatccc caacctgccg cacgaatccg tcccggccgg
     4561 ccgcgatgaa agcgacaatg tggaagtccg ccgggtgggc gagccgcgcg cgtttgactt
     4621 cccggtgaaa gaccatgtcg acctgggcgc cgcgctgggc ctggattttg acaccgccgc
     4681 caagctgtcg ggcagccgct ttgccgtgct caagggccag atggcccgcc tgcaccgcgc
     4741 gctggcgcag ttcatgctgg acacccacgt cagcgaacac ggctacaccg aagtgtacgt
     4801 gccgtatatg gtcaacgccg actcgatgcg cggcaccggc cagctgccca agtttgaaga
     4861 agacctgttc cgcatcccgc gcggcgacga caccttctat ctggtgccca ccgccgaagt
     4921 gccggtgacc aacttcatgc gcgacgagct ggtgaaggcc gagcaactgc cgatgcgcta
     4981 ctgcgcgcac accccgtgct tccgctcgga agccggttcg tatggccgcg acacccgcgg
     5041 catgatccgc cagcaccaat tcgacaaggt ggaaatggtg cagatcgtgc gcccggaaga
     5101 gtcgtttgcc gccctggaag cgctgaccgg tcacgccgaa gccatcctgc aaaagctcgg
     5161 cctgccctac cgcaccatcg tgctgtgcac tggcgacatg ggctttggcg cgtgcaaaac
     5221 ctacgacctg gaagtctggc tgccggcgca gaacacctac cgggaaatct ccagctgctc
     5281 gaatatgggc gacttccagg cccgccgcat gcaggcgcgc ttcaagaacg aacaaggcaa
     5341 aaacgagctg gtgcacacgc tgaacggctc tggcctggcc gtgggccgca ccctggtggc
     5401 ggtgctggaa aactaccaga acgccgacgg cagcgtgacc gtgccggaag cgctgcgccc
     5461 gtatatgggc gggctggaca ccctgcgccc gtaatcggaa cggcgcatgg cgagcgatgc
     5521 acggtcacgc cgtgcatcaa aaatttgccc ccggccagtg ttctggcgcg ggggcagatt
     5581 ttttgattcg nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn cgctcagcgc
     5701 gcgctcatcg cctcccccat ccgcaccggc cgcgccggaa cccaatccgg gttgaaatcc
     5761 agcatgccct tggggaacag catcaccacg gtggagccca gcagaaagcg gcccatctcc
     5821 tcgcccttgg ccagctgcgg cgcatcggcg ccgtcgtagc gccattcgcg cacaaggccc
     5881 gggcgcggcg ggttgatcac gccatgccag gtggtggcca tgctgccgac aatggtggcg
     5941 ccgaccaggg tcagcacaaa cggaccatac tctgattcaa acacgcacac cacccgctca
     6001 ttgcgggcaa acaggccggg cacgccgcgc gcggtggtcg ggttcaccga aaacagcgcg
     6061 ccgggcacgt agatcatccg cgtcagccgg ccagcgcacg gcatgtggat gcggtgataa
     6121 tcgcgcgggc tcaggtagag cgtggcaaac gcgccatcct gaaactgcgc cgccagcgcc
     6181 gcatcgccgc ccaccagcgc ggtggcgctg tagtgatggc ccttggcctg aaaaatctgc
     6241 tcgccggcaa tcggcccaca ctggctgatg gcgccatcca ccggacagac caaggccgtc
     6301 tgcgccagcg gccgcgcgcc ggctttaagc gggcgggtaa aaaacgcatt aaagctgggg
     6361 tagctggcga tgtccgggtt ggccgcttcg tgcatgtcca cctgatagcg ctgcacaaac
     6421 cagcggatca gctgcgtggt caggccgccg gcctgggctt gggccacttt gccggccagc
     6481 gcagtcagcg cctgtttggg caacaggtat tggggcaata cggcaagacg gtcggacacg
     6541 gcggcggcct gagaatgaga aaaagggcgc ttattatagc cagattgccg ggaaaagcgg
     6601 tttgatgcgc ggcaaaaggc cgcgcgatgc agagggcgcc aaaacaaaaa gcccgttgat
     6661 tactcaacgg gctttttcgc caaattctgg cggagagggg gggattcgaa cccccgatag
     6721 gctattaacc tatacacgct ttccaggcgt gcgacttaaa ccactcatcc acctctccgg
     6781 gacaagagct gcgcattata gccagggttt tttctgtgcg caagcccctg cgcattctgc
     6841 gagggcaccg cttaaagagt cgctcacaaa acccagcgca gcggccctgg ccaggcgcac
     6901 gaccgcagac agtacgagag aagtacggca aggtcacaca acgatgccag ggggggtgtg
     6961 agcggcgcta agcccatatt tcatgccccc tactggctct ggccagagtg gggcatattt
     7021 tctgtatttt catgccatat atcttctgcg tttttatgtc atatatatat tgtgatatgc
     7081 acgagacaaa gcacgtgctt taagtaattt tacctatatc ccatctaggg gaaactacgt
     7141 agaacatact gattttgtag ggaaatttgc aaaaatacaa cagtttttta tgatgaggtt
     7201 gactcagcag ggaagtaggt ctacattcaa tatagttata aaacatgctt tggtattttc
     7261 atttaatcga gggatattga ccatgaaaaa agcgatactg atttgtgcaa tgctggggtt
     7321 tgctgcacag agccatgcag tggtggggtc gtggagcaag caaactgcgc attacccgat
     7381 ctacttcagt ggcggctatg cagaaatgcc gttcaatgcg ccagcttcca tgcctacatt
     7441 gacttctttt gtaacgtcgg tttcgtggca gtggcaatct tatgggaatg gcagtagttc
     7501 agaaattgta cttatctgtt attcaaaaaa atattctaat gttattggta actgcaatag
     7561 aatcaatgct cagcagggca gtactagtgt atttaatggc tttgatgcct ccggaaaatt
     7621 ctggatcaaa catactttaa caggagggaa ttaccctgcg atatcgaatg gctttgatca
     7681 agtcaccgtg aatcatcaat aatttgtatc tctatagata taggttataa gtggccagta
     7741 ttccgccatg gggtgtccat ggcggtctct attgctacag cagttttcaa aattttattg
     7801 aatgcttagg taacatgatc tttgttggtg ttttaaactt atttaaggat gctaaaccat
     7861 gaacaatatt tcttccttgg gcgcaagcaa tagtgcatac caagcaaaaa atggtgctat
     7921 agcttctgct gcatctgctg atgcttcagc aaaaaatcag ccttcatcca gtgcacaagt
     7981 cactttgagt ggtaaagcgg tcatgctgag ccggctgtgg cgaattaatg atccttctgc
     8041 ctctgcacca aaagtattgt cctctgttac cactaatatg ttgaccggta attcttatga
     8101 ttttctctca agctcagatc gcaaatttct tgagaaggta tatgcctatg cacaagaaaa
     8161 gaatattgac ctggcccagg tagataatct ggctactgat atggctgcat atagaatgct
     8221 gtatgctaca aatggccctg gcctgagcgc aattggcccg ggcaatattt ttactacaga
     8281 tggcaaggaa attgtttatg cattcaatga gagtgactac tatactgctg agcgtatcaa
     8341 atcagattca gcgttgtctt ctaccgaagt tgatggtgga tttttaagat atgctctgga
     8401 tccatggcag aattctgttc atgctgtaga ttttaaattt ctggaaagag tggtgattga
     8461 tttttctgcg gaaacaggta gatctatcaa tgaaaataca gtaacagcag gtaagggtag
     8521 ttcagatctc cattttggtg taaatgggta ctacgttaaa gaaaaatcca gtacttccag
     8581 tagcacatcc gacaaggcca caaataatac acctaatgta ttaagttatc tgtctgagca
     8641 agacagaaag caattgattg gtgcttataa aatgctgatg tcgctgggta tgagcaccga
     8701 gcagctggat caggccgcca cgcgcttagg gtcaatgcgc atgcatacgg tgactggaca
     8761 atccagcgca agcagcgatg cctcagcgca aactgccaaa gctattgggg cttaccatcg
     8821 ttcggatagt gccttttcac tgtttgatgg catgcttcct gccaacaaac caaagaacca
     8881 cacgcccatt gtccaaatgt cgagtgtgct ggaaaagctg ctgatggcgg aaaaggccga
     8941 gtaaggttaa agcacgagta ttactcaaca ccagatggcg gcaaatggga gctggccgcc
     9001 ttttcttaac gcttacagag agccatcatg agcataagtg caataatcag cccaacatca
     9061 tccgaggtgc aactgtctaa atcatcggca gcaagcacca gcgctcaaga gtctcgagtg
     9121 tcccgtggtc ataccttgat gcttgagcgc ttgtggcgca tcaaggatcc ttctgctcct
     9181 gagccgaaaa ttctatccac tgtcactact gacatgttgt caggcaattc atttgacttt
     9241 ttatcatatt cggatcgtcg gttgctggaa aaagcctaca cttacgcaga agacaataat
     9301 attgacttgg agcaggtgga tttgctggct tttgatatgg caggttatcg tatggcaggt
     9361 cctggtgggc atattccaaa taaaccaggg aactcctata cgctggatgg caagtggata
     9421 ataaatgcat tcgacgagcg agataccaaa attgcttacc aaatcaaaaa tggcaaggca
     9481 atgttgagta ctgagtttga tcgggggttt ttgaatgaag cactagaccc agaaaaagcg
     9541 cctacgcatg gcgttgattt tgcctttcta gccaagatgg tgaaagagtt ttccgaccaa
     9601 gccggccagc cagatgaaaa tctccagatg ccagatgacc ccaatcgtcg ctatgctgaa
     9661 ccggggcgag ggtcgtatct ggtcgaaacc ctcaacggca aaaagccgcc cacccatccg
     9721 gataatctgg gcaaaaatgc cgatggcagc ggggatgtgc tgaattacct gaccaagcaa
     9781 gaccgcaaga agctgttaca ggcttatcag atgctgatgt cgctgggcat gagcaccgat
     9841 cagcttgacc aaaccgccac cagcctgggg gcggtgcgca tgcgcgaagc caccggccaa
     9901 tcggccccgg ccagcagcac aagcgctacc cataaagcca cacgcgcata cagcagtcag
     9961 aacggtttga cctcactgct ggataccctg agcccggcta atcggcagaa aaaccgccag
    10021 ccggtcacgc aaatgtccaa ggtgctggaa aagctgctga tggcggaaaa ggccgagcag
    10081 gcagggcggg cttgaccggg ggcgtgaggg gggcgccgct ttattctcaa accggccaca
    10141 tcagcgcggt ggtcaccagc aaaaagccga tgaacacgct gcgcagccag cgttccggca
    10201 gacggcgggc gcaggccaca ccccaggaaa tggtggagag cccacccagc gccatgggca
    10261 ggcccagcga ccagctgacc tgcccggcgc tggcgtaggc gctgagcgca atcagggagc
    10321 caggcaccac cagcgccagc gccagccctt gtgccgccgc ctggcgatgg ccaaagccgc
    10381 gcaccagcat tggcgtggcc accagcccgc cgcccacggt aaacagcccg gaaaataccc
    10441 cactgaccac ccccaccacc ggcagataac gggcggataa cacccgtggt tgcggcgcgt
    10501 gcgccagggg tgggcgtgcc accagccagc acaggtaagc gcacagccca agcaagaagc
    10561 cgataaacac ccattgcagg gtgtgatggt ccagccccac cgccagcctt gccgccgggt
    10621 aggaggccag catggcaatg ccgcccagcc ccagcgcccg gctcagtggg atggtgtggt
    10681 gctgacggta gcggtaaaac gccagcagca cattgggcac aatcatcacc agcgcggtgc
    10741 cttgcgccat ggattgatcc atgccaaacc atagcgccag caccggaatg gccagcaatc
    10801 cgccgccaat gcccagcagg ccaccaatgc cgcccagcgc cgcgcccagg cccgccatag
    10861 ccagcgccgt ccccagcatg ccccaatgcg ccatcatgac aaaatccttg ttcagcaaac
    10921 tcagcagccg acagtgtaat ccattctccc aacgctataa tccacgctat tgacaagcta
    10981 ttattgtgct ggaggtcaac aatggacgga tggtcagacc tggcgttttt tgccgcgctg
    11041 ctgcgcgccg gcagcctgac cgcgctggcg cgcgagcaag gcgttacccc gccggtgatc
    11101 agccgacggc tggcggcgct ggaggcgcga ctgggcatgc gcctgttgca gcgcaccacc
    11161 cgacgcatgc atttaaccca ggaaggcgag ctgtacgccc agcgcggcca ggtgctgctg
    11221 gccgagctgg aagcgctgga agccgacgtc agcgcccgcc accaaaccgt cagcgggctg
    11281 ttgcgggtga atgcctcgtt tggctttggc cgccgccaca tcgccccggc ggtggcggcg
    11341 ttttcgcgcc agcatccgcg cgtcagcgtg caactgacgc tgaccgaccg gccgctgaat
    11401 ctggtggaca gcggctttga cctgggcatt cgcgtcggtg gcctgcccga cagccgcgac
    11461 cacgcctgcc tgctggccgg caatcgccgg ctgctgtgcg ccgcgcccag ctatctggca
    11521 cgcaacccgg cgcccgagcg gcccgaggcg ctcagccggc atcaatgcat tgtcattcgg
    11581 gaaaacaatc aggcgtatgg caactggcag ctcagccgcg ccggccagca ggtgacggtc
    11641 aaagtcactg gcgggctgtc caccaacgac ggcagcaccg cgctggcctg ggggctggcc
    11701 ggccacggca ttttgctgcg ctccacctgg gaaatcgccc cgcatctgcg cgtgggcgag
    11761 ttgattgaag tgctgccgga ctggcgcttt gccccggccg acgtgtatgc cgtctacccg
    11821 cagcgcgcgc aagcgccggc caagctcagc cggtttgtgg cgtttttgca ggcgcatttt
    11881 gccgcgcacc gggacgggtt ggcgtggtga caccccctag cccttcggca gcatcaacac
    11941 tacggtaatc accaagaaca ccagaaaaat cccacgcaat ttacgatccg gcaggcgctg
    12001 ggccagcgcc acgccgcgcg agatggtgga cagcccgcca atcgccagcg gaatgcccag
    12061 ctgccagtcc accacgccgg cgtgggtgta ggcgcccatg gccaccagcg agctgggcac
    12121 aatcatgcac agccccaggc cctgcgctgc cgcctggcgg tagccaaaca ggcccaccag
    12181 catcggtgag gaaattgtgc cgccaccgac ggtaaacaaa cccgaaaacg cacccgccac
    12241 caccgacacc aagggcagcc agcgctcgga cagtatgcgc ggcgccggcg cacccttggc
    12301 gcgacgggcg tacagctgcc agcccagata gcccaccagc cacagcaaaa agccgatgaa
    12361 aatccagcgc agggtgtgat ggtccagcga caccgccagc cgcgcggccg ggtaagacac
    12421 cacaatcgac accgcgccca gcgtcagcgc ccgccgcagc gcaatcgggt gatgctggga
    12481 atagcgccag aacgccagca ccacattggg cgc
//
LOCUS       DAOPYX010000049        12061 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_5940_length_12061_cov_10.796019, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000049 DAOPYX010000000
VERSION     DAOPYX010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 12061)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12061)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12061
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5940_length_12061_cov_10.796019"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            300..1448
                     /gene="ssuD"
                     /locus_tag="PKN55_04070"
     CDS             300..1448
                     /gene="ssuD"
                     /locus_tag="PKN55_04070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018747175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008726 - alkanesulfonate monooxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
                     /protein_id="HNX70451.1"
                     /translation="MSLDIFWFLPTSGDTRYLGRADAARPLNNAYLKQIAVAVDQLGY
                     DGILIPTGSGCLDPWVTASTVATVTQRIKLLVALRTALTQPTAAARQAATLDQVSNGR
                     LLVNVVAGGDAIELAGDGIFLDHDARYEEAREFLTIWRQLLAGETVNFSGKYHTVKDA
                     RNFFPPVQTPHPGIYFGGSSPAAHDLAAEQVDAYLTWGEPPAAVAEKIADVRARAAQH
                     GRTVRFGVRLHVIVRETNEAAWAAADALIEHVDEATIAKVQARYAQMDSEGQRRMAAL
                     HNGRRDQLEVSPNLWAGVGLVRGGAGTALVGDPHTVAARLKEYADLGVDSFVLSGYPH
                     LEEAYRFAELVFPLLPGKAELRPAGKIVTGGPFDASTLADVPAEQEAA"
     gene            1445..2305
                     /locus_tag="PKN55_04075"
     CDS             1445..2305
                     /locus_tag="PKN55_04075"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNX70452.1"
                     /translation="MNVIDMRCRPAYLHDFFGATPGTAAYETARWLNRRVGSRHDEHF
                     TPSLTREGFLQEVHAAGLRHAVVVGRHTPGQHLPNDRIQQIVADDPVLLGVGAVDPIV
                     QGVQPALDELERAVNSLGLIGIDVEPGFGQPARHADDRAYFPVYEACAALNVPVFIMS
                     GPTTPDPSYNDPAAIGRLAQAFPTLPIVCYHGFYPNVAGILGVAFRHENVYVVPDMYL
                     FVAGARDYVEAGNGFLREQLLFGSSYPFRPIGQSIDDFLALGFKDGAVEAVLHDNAAR
                     LLKLGPAPAR"
     gene            2329..3288
                     /locus_tag="PKN55_04080"
     CDS             2329..3288
                     /locus_tag="PKN55_04080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012206439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HNX70453.1"
                     /translation="MTDAFQPSRRRLLGALSAAALAAALPRPARAASDTLRIGYQKYG
                     TLVLLKAQGTLEKRLAPLGIQVKWAEFPGGPQLLEALNVGSVDFGTTGETPPIFAQAA
                     GADLQYVAYEPPAPKGEAIVVAKDSPLKSVKDLKGKRVALNKGSNVHYLLVKALEKAG
                     LKYEDVQTVFLNPSDARAAFERGSVDAWVIWDPFLAAAEHQLGARQLADGVGLVSNHQ
                     FYLASRRYAEQNGKVIKLIVEELARLDAWARQNFKDVAGQLSSQIGLDVNIVRVAAER
                     GTYDVRLLDAKVIAEQQRIADVFHQLKLIPKPLSVKDIVWSGQ"
     gene            complement(3465..4289)
                     /locus_tag="PKN55_04085"
     CDS             complement(3465..4289)
                     /locus_tag="PKN55_04085"
                     /inference="COORDINATES: protein motif:HMM:NF024701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNX70454.1"
                     /translation="MPSSGPRIPRKPAGRYHHGDLRRALIDASLELVRQRGPSGFTLA
                     EICRAAGVSQAAPYRHFEGKDHVLAVAASEGYQLLHAAMAGIAREPNQLNETLERMAR
                     AYLNFAIRHPAHLAVMFSHYPGDHFGGLLKEPNHGGPDGLKNLPPPQNQTEEAILASW
                     HAGQAGFQALTQAILTAAQGSPMAARINETTASHYSAAIWSMVHGLAMLLLERMIPPE
                     WMQDDFKLAIELVVRPWINGLADMPTPVLSQMVNCPRLHMMGVQLPALADPAHPAG"
     gene            4594..5595
                     /locus_tag="PKN55_04090"
     CDS             4594..5595
                     /locus_tag="PKN55_04090"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014087674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HNX70455.1"
                     /translation="MSQIPQTLTHWLIDAQRKAGGDSVMLQLLDDIATACKMISHEIA
                     QGAMLGNLGKAGSENVQGEEQKQLDVIANDIFLAVNAQAGHYPAMASEELEDVYVVDG
                     AAEGKYLLLFDPLDGSSNIDVNVSVGSIFSILRLPEGTDPSQAAAFLQTGVQQVAAGY
                     ALYGPSTMLVISVGQGVHGFTLDRNTGLFYLTHPDMSIPADTKEFAINASRARFWEAP
                     VKRYVDECLAGKTGPRGRDFNMRWIASMVAEVHRILTRGGLFMYPMDAENQAKGGKLR
                     LMYEANPMSFLVEQAGGAASTGRQRIMDVAPTGLHQRISVILGSKNEVERVASYHAE"
     gene            6154..>6451
                     /locus_tag="PKN55_04095"
     CDS             6154..>6451
                     /locus_tag="PKN55_04095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014088301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HNX70456.1"
                     /translation="MPINQTEYRVDPKQPIVTRTDLKGVITYANPAFVALSGFSEKEL
                     IGHSHNVVRHPDMPPEAFDDLWQTLKAGSPWQGIVKNRAKDGGFYWVDAYVTPVT"
     assembly_gap    6452..6551
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6552..7892
                     /locus_tag="PKN55_04100"
     CDS             <6552..7892
                     /locus_tag="PKN55_04100"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNX70457.1"
                     /translation="RAKDGGFYWVDAYVTPVTENGQTIGYMSVRNAPSPQQISEAERL
                     YAAIRNKQAKMPSTLARARRLPSFSRRMWGAGLLCGALAGAANLVLDKMGLAWMSVPL
                     GALLGIAPMAWLFPQLTRTLNEVSAGFINLAEGKLNMPVRQSWHCALGDVQVRLESMR
                     IYQKAVVADVITEAQANSASANMLNANLGQLVQCFHAQNVMLAEMAAQLDSVGSSVKE
                     IVAVSDAMSHEAEHTRERVAEGDVTMLDISSSSRNTLDAMTHSCASMQALRQTVAKIQ
                     EMSALIDDIAGQTNLLALNASIEAARAGEHGRGFSVVADEVRKLAERTVSSNQSIQDT
                     LQSIHSHVFEAFGRTEKAMGTVEHSQQAIGHFSQGLNNIRDAAEHALQQARALLGFTS
                     QQSEATQDVVVHLNEVFASLERNEQTAVDLQQQAGRLSQSSLSLNKLLGHFRIA"
     gene            8090..9844
                     /gene="ggt"
                     /locus_tag="PKN55_04105"
     CDS             8090..9844
                     /gene="ggt"
                     /locus_tag="PKN55_04105"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HNX70458.1"
                     /translation="MLTLSFRHWASAALLAGGLLGSLPTLADQPPPEAASGFARITAP
                     TAFDHAMIVTANPLASDAGAAILRQGGSAVDALIAAQWVLGLTEPQSSGLGGGGFLLT
                     HDGKRTLQSWDGRETAPAAARGERFIGADGKPLPFSQAVASGKAVGVPGLVAMLYAAH
                     QQHGKLPWAALAQPAIQLAEQGFAVSPRLYTLLVQETRLQADANARRYFFSEDGRPRP
                     VGSLLRNPDYADTLRRIARQGPAGFYQGPVAADMVRAVQNHPTPGDLTLADLASYRPK
                     QRAPLCGPYRQWQVCGMGAPSSGGVAVLQLLGMLARFQLNTLPPTSADAVHLYSEAGR
                     LAFADRERYLADPDFVAVPQAGLIAPDYLAARSQLIDPQRSHGPARAGEPAGVKLSAW
                     GRDAAPELPSTTHLVAFDRDGRAASMTSSVEDAFGSRIMVRGFLLNNQMTDFSFLPEQ
                     DGAPVANRIQPGKRPRSAMSPTLVLDQQGQVQMAVGSPGGSLIIHYVAQALLGLLEWR
                     LDPQAAANLPHYGSRNGPTELEADQQLERLIEPLRQRGHEVKLTPMTSGLSIIVRQPQ
                     GGYLGGADPRREGKVSGF"
     gene            10058..10540
                     /locus_tag="PKN55_04110"
     CDS             10058..10540
                     /locus_tag="PKN55_04110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX70459.1"
                     /translation="MMEWNVYAIARALHVLGVVLWIGGVAMVTTVLLPAIRRSTPPAE
                     QYRLFERIEHRFAAQARWSTQLVGASGLAMLWLTDSWARLANTGWLHLMLAVYAVFTL
                     MLFVLEPLLIHRLLAGWARRTPGAAMRRLQRMHWLLLAISLAAVVFGVVGAHGGWQLG
                     "
     gene            complement(10826..11710)
                     /locus_tag="PKN55_04115"
     CDS             complement(10826..11710)
                     /locus_tag="PKN55_04115"
                     /inference="COORDINATES: protein motif:HMM:NF013412.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HNX70460.1"
                     /translation="MTAFHAGEQALQALHGQRERLAEVGARVVRPAMPDAHRAFFAEL
                     PWLLAGAVDADGQPWASALCGAPGFIHSPEADRLDIAAPLAAHDPLRACLRVGARIGL
                     LGLQPHSRRRNRANGQVAALTPQGLSVAVAQSFGNCPKYIHPRTLNWQPAASAPTLHC
                     AATLDARAQALIRQADTCFIASAHPEDDGQPAHGVDVSHRGGPPGFVQVVDEQRLWLP
                     DYSGNGYFNTLGNLHLHPRAGLLWLDFVHGDMLWLAAQAELVGAEGAASDAQQNGRGV
                     RLTVREVRYAPGALAVRE"
     gene            complement(11728..>12061)
                     /locus_tag="PKN55_04120"
     CDS             complement(11728..>12061)
                     /locus_tag="PKN55_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011135327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutathione binding-like protein"
                     /protein_id="HNX70461.1"
                     /translation="PIARCSRWRQVRAGGAARLITVFGAKFNPEEVIARAHALFVILD
                     AELAQRDWLVGEQVTIADIALYSYTAHAPEGNVALDAYPHIRAWLARIEALPGFVAMP
                     ATAAGLRA"
BASE COUNT         2093 a         3878 c         3844 g         2146 t
ORIGIN      
        1 ctgcccacaa aacgctcttg gctttgttgc ccagccttgc cgtgcttctg gctctgtcgg
       61 cgtgctggcc aggatcgctg cgctgggttt tgtgggcggc atgctaagtt gcacaagatg
      121 ccgggcagta tacccgcact ggccagccaa cctgttatga caggttataa ctttatctgc
      181 aaagctttgc agttttcctc gtttccgccg gctggccggc gctgcctaga atcctcgcca
      241 tcccctgacg cgactgtcgg ccccgccggc ggctgcccac ctgtaatgag gaatgtgcga
      301 tgagtcttga tattttctgg tttctgccca cctcgggcga cacccgttac ctgggccgcg
      361 ccgacgccgc ccgcccgctg aacaatgcgt atctcaagca gatcgccgtg gcggtggatc
      421 agctcggtta cgacgggatt ctgattccca ccggcagcgg ctgcctggac ccctgggtga
      481 ccgcctcgac cgtggccacc gtcacccagc gcatcaagct cctggtggcg ctgcgcaccg
      541 cgctgaccca gcccaccgcc gccgcccgtc aggccgccac gctggatcag gtcagcaatg
      601 gccgcctgct ggtcaatgtg gtggctggcg gcgacgccat cgagctggcc ggggacggga
      661 tttttctgga ccacgacgcc cgctacgaag aagcccgcga gtttctcacc atctggcgcc
      721 agctgctggc cggcgagacg gtgaatttct ctggcaaata tcacacggtg aaagacgcgc
      781 gcaatttctt cccgccggtg caaaccccgc atccgggcat ttattttggc ggctcgtcgc
      841 cggcggcgca tgacttggcc gccgaacagg tggacgccta cctgacctgg ggcgagccgc
      901 cggccgccgt ggcggaaaaa atcgccgacg tgcgcgcccg cgccgcccag catggccgca
      961 ccgtgcgttt tggcgtgcgc ctgcatgtga ttgtgcgcga aaccaacgaa gccgcctggg
     1021 cggcggccga tgcgctgatc gagcatgtgg acgaggccac catcgccaag gtgcaggcgc
     1081 gctacgcgca gatggattct gaaggccagc ggcgcatggc cgcgctgcac aatggccggc
     1141 gcgaccagct ggaagtcagc cccaatctgt gggcgggcgt tggcctggtg cgcggcggcg
     1201 ccggcaccgc gctggtgggc gacccgcaca ccgtggcggc gcggctgaag gaatacgccg
     1261 acctgggcgt ggacagcttt gtgctgtccg gttacccgca cctggaagaa gcctaccgct
     1321 ttgccgagct ggtgtttccg ctgctgccgg gcaaggccga gctgcgcccg gccggcaaga
     1381 ttgtcactgg cggcccgttt gacgccagca ccctggccga cgtcccggcc gaacaggagg
     1441 ccgcatgaac gtcatcgaca tgcgctgccg gccggcctat ctgcacgact tttttggcgc
     1501 cacgcccggc acggcggcgt atgagacggc gcgctggctt aaccgccggg tgggctcgcg
     1561 ccacgacgag cacttcaccc cgtcgctgac ccgcgagggc ttcttgcagg aagtccacgc
     1621 cgccggcctg cgccacgccg tggtggtggg ccgccacacc cccggccagc acctgcccaa
     1681 tgaccgcatc cagcagattg tcgccgacga cccggtgctg ctgggggtgg gcgcagtgga
     1741 cccgattgtg cagggcgtgc agccggcgct ggatgagctg gagcgcgcgg tgaacagcct
     1801 ggggctgatc ggcattgatg tcgagccagg ctttggccag ccggcgcgcc acgccgacga
     1861 ccgcgcgtat ttcccggtgt acgaagcctg cgcggcgctg aatgtgccgg tgttcatcat
     1921 gtccggcccg accacgcccg accccagcta caacgacccg gcggccatcg gccggctggc
     1981 gcaggctttc cccaccttgc cgattgtctg ctaccacggc ttttacccca atgtcgccgg
     2041 cattctgggc gtggccttcc gccacgagaa tgtttatgtg gtgccggaca tgtatttgtt
     2101 tgtggcgggc gcgcgcgatt acgtcgaggc cggcaatggc tttttgcgcg agcaattgtt
     2161 gtttggttcg tcctacccgt tccggccgat tggccagtcg attgacgatt ttctggcgct
     2221 gggcttcaag gacggcgccg tagaagcggt gctgcacgac aacgccgcgc gcttgctcaa
     2281 gctgggcccg gcgccggccc gctgaaccga ccccctggag aacacatcat gaccgacgct
     2341 tttcaaccgt cccgccgccg cctgctgggc gccttgtccg ccgccgccct ggccgccgcg
     2401 ctgccgcgcc cggcgcgggc cgccagcgac accctgcgca tcggctacca gaaatacggc
     2461 acgctggtgc tgctgaaggc gcaaggcacg ctggaaaaac gcctggcccc gctgggcatc
     2521 caggtgaaat gggcggaatt ccccggcggc ccgcaactcc tggaagcgct gaacgtcggc
     2581 agtgtggact ttggcacgac gggcgaaacc ccgccgatct tcgcccaggc cgctggcgcc
     2641 gacttgcaat atgtggccta tgagccgccg gcgcccaagg gcgaggcgat tgtggtggcc
     2701 aaggattcgc cgctgaaatc ggtgaaagac ctcaaaggca agcgcgtggc gctgaacaag
     2761 ggctcgaatg tccactacct gctggtcaag gcgctggaaa aagccgggct gaaatacgaa
     2821 gacgtgcaaa ccgtgtttct caacccgtcc gacgcgcgcg ccgccttcga gcgcggcagc
     2881 gtggacgcct gggtgatctg ggatccgttc ctggccgccg ccgagcatca actgggcgcg
     2941 cgccagctgg ccgacggcgt cgggctggtc agcaatcacc agttttacct ggcctcgcgc
     3001 cgctatgccg agcaaaacgg caaggtgatc aagctgattg tggaagaact cgcccgcctg
     3061 gacgcctggg cgcggcaaaa cttcaaggac gtggccggcc agctgtcctc gcaaatcggc
     3121 ctggacgtca acatcgtgcg ggtggccgcc gaacgcggca cttacgatgt gcgcctgctg
     3181 gacgccaagg tgattgccga gcaacagcgc atcgccgatg tgtttcatca gctcaagctg
     3241 attcccaagc ctttgtcggt gaaagacatt gtctggtcgg ggcagtaagc gccgctcaca
     3301 aaaccctcct ggcgttgttg tacggtcttg ccgccctgct tgactgtctg cggtcgtgcg
     3361 cccggtcagg gccgctacgc tgagttttgt cagccacgcc cacacacaca ggcagacgaa
     3421 cacaggccca cgccatacag cgtgggcctg tgtgcattgg cggctcagcc tgccgggtgg
     3481 gcggggtcgg ccagtgccgg cagttgcaca cccatcatgt gcagacgcgg gcaattgacc
     3541 atctggctca gcactggagt tggcatgtcg gccaggccgt tgatccatgg ccgcaccacc
     3601 agttcgatgg ccagcttgaa atcatcctgc atccattctg gcggaatcat gcgttccagc
     3661 agcagcatgg ccagaccgtg caccatcgac cagatggccg ccgaataatg gctggccgtg
     3721 gtttcgttga tgcgcgcggc catcggcgag ccttgcgctg cggtcaggat ggcctgggtc
     3781 agcgcctgaa aaccggcctg gccggcatgc cagctggcca gaatggcttc ttcggtctgg
     3841 ttttgcggcg gcggcaggtt tttcaggccg tccgggccgc catggttggg ctctttgagc
     3901 aagccaccaa agtgatcgcc cggataatgg ctaaacatca ccgccaggtg cgcggggtgg
     3961 cggatggcaa agttcaggta ggcacgcgcc atgcgctcca gggtttcgtt cagttgattg
     4021 ggctcgcggg caatgccggc catggccgcg tgcagcagct ggtagccttc gctggcggcc
     4081 accgccagga cgtgatcctt gccttcgaag tgccggtagg gcgcagcctg gctgacgcca
     4141 gcggcccggc agatttcggc cagggtaaag ccagacgggc cacgctggcg caccagttcc
     4201 agcgaggcat caatcagcgc gcggcgcaga tcgccatgat gatagcggcc ggcaggctta
     4261 cgcgggatgc gaggtccgga tgacggcatg atgaagacac tcatacaaat gtgtaatgag
     4321 gcatatctta ttgcaatgcg cggagcgctg acagtctgtt ctacttatgt taatatacaa
     4381 tacatctcca aggccttgct ggcattgccc atttgccaac aagacgtctt tgcaggatgc
     4441 gtgttgatac cccgacagct ctccggctga tcggggtttt tttcggcgat accctggccc
     4501 ggcggcccag ggttttggcg ctataatgac ggccaatcca aacaccctgg gctgcattgg
     4561 cggccctttt tgccattcca caaggttttc gacatgtccc agattcccca gaccctgacc
     4621 cactggctga tcgacgccca gcgcaaggct ggcggcgaca gcgtgatgtt gcagctgctt
     4681 gacgacatcg ccaccgcctg caagatgatc tcccacgaaa tcgcccaagg cgccatgctt
     4741 ggcaatctgg gcaaggccgg tagcgagaat gtgcaagggg aagagcaaaa acagctggac
     4801 gtgattgcca acgatatctt tctggcggtg aacgcccagg ccggtcatta cccagcgatg
     4861 gcttcggaag agctggaaga cgtctatgtg gtggatggcg cggccgaggg caagtatctg
     4921 ctgctgtttg acccgctgga tggctcgtcc aatatcgacg tgaatgtctc ggtgggcagt
     4981 attttctcca tcctgcgcct gcctgaaggc actgacccca gccaggcggc cgccttcctg
     5041 caaacgggtg tgcaacaagt ggctgccggc tatgcgctgt atggcccgtc caccatgctg
     5101 gtgatcagtg tcggccaggg cgtgcacggc tttaccctgg atcgcaacac cggcctgttc
     5161 tacctgactc acccggacat gagcatcccg gcagacacca aggaatttgc catcaacgcc
     5221 tcgcgtgccc gcttctggga ggcgccggtc aagcgctatg tggacgaatg cctggccggc
     5281 aaaaccggcc cgcgtgggcg tgatttcaat atgcgctgga ttgcctcgat ggtggccgaa
     5341 gtgcaccgca tcctcacccg tggcgggctg ttcatgtacc cgatggacgc cgaaaatcag
     5401 gccaagggcg gcaagctgcg cttgatgtat gaagccaacc cgatgtcctt cctggtggaa
     5461 caagccggtg gcgccgccag caccggccgc cagcgcatta tggatgtcgc gccgaccggc
     5521 ctgcatcagc gcatttcggt gattctgggc tcgaaaaacg aagtcgagcg cgttgccagc
     5581 taccacgccg agtaagcgtc gtactgcgtc cgcaccacgc gcgcagccca tccgggttgc
     5641 gcgtttttta ttggcacaga atattgtgtc aatgctttgt actattcaat aaatggcatg
     5701 tactggacaa aatacaccgt aatgaattca atatgtcagg tacttgccct ggcaatgggg
     5761 cagtgatacg ggctggcgtg cgttcagttt gacaaggctt atccgttttg cgcacaccat
     5821 cccccattaa tgtcatatga attttgtgca ccgcaaaaca accaacagtc aagtgtttca
     5881 gaaataggtc cctcccaacc agcctctcta agcattcgta cgaattgaaa cccaactggg
     5941 ctctgctata gtgaaacaca gggcactatc ttgcaatgca caacatgcgg gagccctgat
     6001 ggggtgggag agttgcacat aaagcgtcag gtttgtacgg catcctgaga aaaacaatag
     6061 ccaagccacc cacttttacc gttctgtttg ctgcttttct tgactgacgt ggcaagcgct
     6121 gacaaccgga gaggtgccca catgaaaatc aacatgccga tcaatcaaac cgaatatcgg
     6181 gttgatccca aacagcctat tgtcacccgc actgatctta aaggggtgat tacttatgct
     6241 aacccggcct ttgttgcgct aagcggtttt agcgaaaaag aattaattgg tcatagccat
     6301 aatgtggtgc gccacccgga catgccgccc gaagcctttg acgatctgtg gcaaacgctg
     6361 aaggctggct cgccctggca gggcattgtg aaaaatcgcg ccaaggatgg cggcttttac
     6421 tgggtggacg cctacgtcac cccggtgacc gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6541 nnnnnnnnnn ncgcgccaag gatggcggct tttactgggt ggacgcctac gtcaccccgg
     6601 tgaccgaaaa cggccagacc attggttata tgtcagtgcg caatgcgccg tccccgcagc
     6661 aaatttccga agccgagcgt ttgtacgccg ccattcgcaa caaacaagcc aaaatgccat
     6721 ccaccttggc gcgcgcacgt cggctgccgt cgttttcccg gcgcatgtgg ggggcggggc
     6781 tgctgtgcgg cgctttggct ggcgccgcca atctggtgct ggacaagatg ggcctggcct
     6841 ggatgagcgt gccgctgggc gccttgctgg gcattgcgcc gatggcctgg ctgtttccgc
     6901 agctcacccg cacgctgaac gaggtatcgg ccggctttat caacttggcc gaaggcaagc
     6961 tgaacatgcc ggtgcggcaa agctggcact gcgcgctggg cgatgtgcag gtgcggctgg
     7021 aatccatgcg catctaccaa aaagccgtgg tggccgatgt gattaccgaa gcgcaggcca
     7081 acagcgcctc ggccaatatg ctcaacgcca atctgggcca gctggtgcaa tgctttcacg
     7141 cccagaacgt catgctggca gagatggccg cccagctgga cagtgtgggc agctcggtca
     7201 aagaaatcgt cgccgtgtct gacgccatgt cgcacgaagc cgagcacacc cgcgagcggg
     7261 tggccgaagg cgatgtcacc atgctggata tctccagctc ctcgcgcaac accctggatg
     7321 cgatgaccca ctcctgcgcg tcgatgcagg cgctgcgcca gacggtggcc aagattcagg
     7381 aaatgtcggc gctgatcgac gatatcgccg ggcagaccaa tctcttggcg ctgaacgctt
     7441 caattgaggc tgcgcgcgcc ggcgagcacg ggcgcgggtt ttctgtggtg gccgacgaag
     7501 tgcgcaagct ggccgaacgc acagtgagtt ccaatcagag tatccaggac accctgcaat
     7561 ccatccacag ccatgtgttc gaggcgtttg gccgcaccga aaaagccatg ggcacggtcg
     7621 agcacagcca gcaggcgatt ggccactttt cgcaggggct gaacaatatc cgcgacgccg
     7681 ccgaacacgc gctgcaacag gcccgcgcgc tgctgggttt tacctcgcag caatcggaag
     7741 ccacccagga tgtggtggtg cacctgaatg aagtgttcgc ctcgctggag cgcaacgaac
     7801 aaaccgccgt ggatttgcag cagcaagccg gccggctatc gcaaagcagc ctgagcctga
     7861 acaagctgct cgggcatttc cgtatcgctt gagtgtcatg aatcgcctgg ccggccgagt
     7921 caggtggttt actgtggccc gcgcgtctcc ccggtgtcgc gcgggatttt ttgccagcgg
     7981 cctgccgccc atgccacagt gccccgttgc ctcacagcct gcgcctgatg gcgttatcgt
     8041 cgcagagccc gtgcgaacgg cgcaccggcc aggactccag gagtatttga tgctgacctt
     8101 gtcttttcgc cactgggcat cggccgcact gctggccggt ggcctgctgg gcagcctgcc
     8161 cacgctggcc gaccagccgc cgcccgaagc cgccagcggc tttgcccgca tcacggcccc
     8221 cacggcgttt gaccacgcca tgattgtcac cgccaacccg ctggccagcg atgccggcgc
     8281 cgccatcctg cgccagggcg gcagcgcggt ggatgcgctg attgccgcgc aatgggtgct
     8341 ggggctgacc gagccgcagt cgtccggcct gggcggcggc ggctttttgc tgactcacga
     8401 cggcaagcgc acgctgcaaa gctgggacgg ccgggaaacc gcgcccgccg ccgcgcgcgg
     8461 cgagcgcttt atcggcgccg acggcaagcc gctgcccttt agccaggcgg tggccagcgg
     8521 caaggcggtg ggcgtgcccg gcctggtggc catgctgtac gccgcccacc agcaacacgg
     8581 caaactgccg tgggccgcgc tggcgcagcc ggccattcag ctggccgaac agggctttgc
     8641 cgtgtcgcca cggctgtata ccttgctggt gcaggaaacc cgcctgcaag ccgacgccaa
     8701 cgcccgccgt tatttcttta gcgaagatgg ccggccacgg ccggtgggca gcctgttgcg
     8761 caacccggac tacgccgaca ccctgcgccg cattgcccgc cagggcccgg ccgggtttta
     8821 tcaggggccg gtggccgcag acatggtgcg cgcagtgcaa aaccacccca cgccgggtga
     8881 tctgaccctg gccgacctgg ccagttatcg ccccaaacag cgcgcgccgc tgtgcgggcc
     8941 gtaccggcaa tggcaggtgt gcggcatggg cgcgcccagc tcgggcgggg tggcggtgtt
     9001 gcagctattg ggcatgctgg cgcgttttca gctgaacacc ctgccgccca cctcggccga
     9061 cgccgtgcat ctgtatagcg aagccggccg gctggcgttt gccgaccgcg agcgctatct
     9121 ggccgacccg gattttgtcg ccgtgccgca agccgggctg attgcgccgg attacctggc
     9181 cgcgcgcagc cagctgatcg acccgcaacg cagccatggc ccggcccgcg ctggcgagcc
     9241 ggccggggtg aagctgtcgg cctgggggcg cgacgccgcg ccggaattgc cgtccaccac
     9301 ccatctggtg gcgtttgacc gcgacggccg cgccgcctcg atgaccagct cggtagaaga
     9361 cgcctttggc agccgcatca tggtgcgcgg ctttttgctg aacaaccaga tgaccgactt
     9421 ctcgtttttg cccgagcagg acggcgcgcc cgtggccaac cgcatccagc cgggcaagcg
     9481 cccgcgcagc gccatgtcgc ccaccctggt gctggatcag caaggccagg tgcagatggc
     9541 agtgggttca cccggcggca gtctgatcat ccactatgtg gcgcaggcgc tgctgggcct
     9601 gctggaatgg cggctggacc cgcaagccgc cgccaatctg ccgcactacg gcagccgcaa
     9661 cggccccacc gaactggaag ccgatcaaca actggaacgg ctgatcgagc cgctgcgcca
     9721 gcgcgggcat gaggtcaagc tcacgccgat gaccagcggg ctgtccatca tcgtgcgcca
     9781 gccgcagggc ggttatctgg gcggggcaga cccgcgccgc gaaggcaagg tatcaggctt
     9841 ttgacgcgac tcacaaaatc ctcccggcgt ggttgtatga ccttgccgtg tgacttgtac
     9901 tgtctgcggt cacacgcctg gccagaatcg cggcgctggg ttttgtgagc cgcttttagc
     9961 gcagggggcg gcgtgccggc gcttgatgca aatcatggcc gacccgccgc cccggccgct
    10021 atggtgcggg aatccccgcg ccagaaaggc ctgatgaatg atggaatgga atgtatacgc
    10081 cattgcccgc gccctgcatg tgctgggcgt ggtgctgtgg ataggcgggg tggccatggt
    10141 caccaccgtg ttgctgccgg ccattcgccg cagcacgccg ccggccgagc aataccggct
    10201 gttcgaacgc atcgagcatc gctttgctgc tcaggcgcgc tggagcacgc aactggtggg
    10261 cgccagcggc ctggccatgc tgtggctgac cgacagctgg gcgcggctgg ccaacaccgg
    10321 ctggctgcac ctgatgctgg cggtgtatgc ggtgttcacc ctgatgctgt ttgtgctgga
    10381 gcccttgctg attcatcgtc tgctggccgg ctgggcgcgc cgcaccccgg gcgctgccat
    10441 gcgccgcttg cagcgcatgc actggctgtt actggccatc agcctggccg ccgtggtgtt
    10501 tggtgtggtg ggcgcgcatg gcggctggca attgggctga gccgcgcata gcatttttct
    10561 gcgtggcggt acggcctgct tagagtcgct aacaaaaccc agcgcagcgg ccctggctag
    10621 gcgtgtgacc gcagacagta caagcagtac ggcaaggtca cacaacaacg ccagggggtt
    10681 ttgttagcgg ctcttagctg ctcacaaaac ccagcgccgc acgcctggcc aggcgcgcgg
    10741 gcgcagacag tacaagccaa gcagtacggc aaggctgcac aacaacgcca ggagggtttg
    10801 gtgagcggcg ctcacggtgg gcggcctact cgcgcaccgc caacgccccc ggcgcatagc
    10861 gcacctcgcg caccgtcagc cgcacgccgc gcccattctg ctgcgcatcc gacgccgcgc
    10921 cttccgcccc caccaactcg gcctgcgccg ccagccacag catgtcgccg tggacaaaat
    10981 ccagccacag cagccccgcg cgcgggtgca gatgcaaatt gcccaaggta ttgaaataac
    11041 cattgccaga atagtccggc agccagagcc gctgctcgtc cacgacctgc acaaaccccg
    11101 gcggcccgcc gcgatgcgag acgtccacgc catgcgccgg ctggccgtcg tcttccgggt
    11161 gggcgctggc gataaagcag gtgtcggcct ggcggatcag cgcctgggcg cgggcgtcca
    11221 gcgtggcggc gcaatgcagg gtgggcgcgc tggcggcggg ctgccagttg agggtgcgcg
    11281 ggtggatgta tttcgggcag ttgccaaagc tttgcgccac cgccacgcta agcccctgcg
    11341 gcgtcagcgc cgccacctgg ccattggcgc gattgcgtcg gcggctgtgc ggctgcaagc
    11401 ccagcaggcc gatgcgcgcg cccacccgca ggcaggcgcg caaggggtcg tgggcggcca
    11461 gcggcgcggc gatgtccagc cggtcggcct cggggctgtg gataaaaccg ggcgcgccgc
    11521 acagcgcgct ggcccagggc tggccgtcgg cgtccaccgc acctgccagc agccagggca
    11581 gctcggcaaa aaatgcccgg tgcgcgtccg gcatggccgg gcgcaccacc cgtgcgccca
    11641 cttccgccag ccgttcgcgc tggccgtgca aggcctgcaa ggcttgctcg ccggcatgaa
    11701 acgccgtcat cgccattccc cctgcgctca ggcgcgcaag ccggcggcgg tggccggcat
    11761 ggccacaaaa cccggcaagg cttcgatgcg cgccagccag gcgcggatgt gcggataggc
    11821 gtccagcgcg acattgcctt ctggcgcgtg cgcggtgtag ctgtacagcg cgatgtcggc
    11881 aatcgtcacc tgctcgccca ccagccagtc gcgctgcgcc agctcggcgt ccagaatgac
    11941 aaacagcgca tgggcgcggg cgatgacctc ttccgggttg aacttggcgc caaacacggt
    12001 gatcaagcgc gccgcgccac ccgcgcgaac ttgccgccag cgactgcatc gggctattgg
    12061 g
//
LOCUS       DAOPYX010000050        11962 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_6021_length_11962_cov_10.556479, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000050 DAOPYX010000000
VERSION     DAOPYX010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 11962)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11962)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11962
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6021_length_11962_cov_10.556479"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..777)
                     /locus_tag="PKN55_04125"
     CDS             complement(<1..777)
                     /locus_tag="PKN55_04125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008953409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HNX70462.1"
                     /translation="MLTWIKKQLPSSGLTAFITHQDCLLHNVGEGHPECPERLIAIRD
                     QLMASQLYDELHEVEAPEVTDQQLARVHPPHYLESLEASTPASGTYRVDPDTAMMAHT
                     LRAARRAAGGAVKAVDMVCEGLAPNAFCSIRPPGHHAESAKSMGFCFYNNVAIAASHA
                     LSQHGLERVAIIDFDVHHGNGTEEIFQNDPRVMMVSIFQHPFYPYCGDVPLGPNMHNV
                     PMKAGSGSSAFREAVEMAWLPNLEAFQPQMIFISAGFDAHR"
     gene            complement(1151..2107)
                     /gene="rpoS"
                     /locus_tag="PKN55_04130"
     CDS             complement(1151..2107)
                     /gene="rpoS"
                     /locus_tag="PKN55_04130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008953405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HNX70463.1"
                     /translation="MSHSIDSLHDEEVSDLEEPLEEAVADADTSEDVADSSFASMDEL
                     ADVTQIYLNDIGHNALLSADEEKALCRRVVQGDFAARQRMIEHNLRLVVNIAKHYINR
                     GLPLLDLIEEGNIGLMHALEKFDPERGFRFSTYATWWIRQSIERAIMNQSRTIRLPVH
                     VIKELNVCLRAQRHLEMQAGREPTLEEISHLVDKPVEEVRRILSLNERVASLDAPLDI
                     DPMLTVGESIPDEQQEEPDELLHNAEIERYVREWLQQLNDKQRTVIERRYGLNGYEIC
                     TLEELAASMSLTRERVRQIQIEGLETLRRLLRRKGVSKDMLL"
     gene            complement(2161..3327)
                     /locus_tag="PKN55_04135"
     CDS             complement(2161..3327)
                     /locus_tag="PKN55_04135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011137228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HNX70464.1"
                     /translation="MASGSRGAVTVQAAPAGEVSVYRAKAPVKVSPASSTSTTTTASS
                     THIPAYPMNSSQAAPAPTPVPMGSRAPSSRDPDDDRPDSYKVKAGDTLYSIALNYGQD
                     YRDIAAWNQLDDPGVIKVDQVLRLTPPAEPAPARSVAVAPGAVPAAEKPAPTPPVADA
                     GARHGPKAVKLPYSAEAVRKIGSVAEAAPADKSADKSEARSDSKTEKQAKADKPASKT
                     EKDNKVDSKAVDKDKLGKDKERDSSKNTEAPRAPTVAEPAASAQAGEEDVASWGWPTQ
                     GKVINRFTDNNKGIDIGGKTGQPVLASAKGRVVYSGSGLRGYGKLIIIKHNKTFLTAY
                     AHNSQLLVKEGQVVTKGQKIAEMGNSDADQVKLHFEIRRFGKPVDPQKYLGNAP"
     gene            complement(3640..4299)
                     /locus_tag="PKN55_04140"
     CDS             complement(3640..4299)
                     /locus_tag="PKN55_04140"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003222172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HNX70465.1"
                     /translation="MNTPQLGIGMTSPRTRTRMVDRLREKGIADARVLAAMAELPRHL
                     FVENALAHRAYDDDALPIGHGQTISQPFTVARMTELLLNGKSPRKILEIGTGCGYQTA
                     VLTKLFPEVYSIERIAALLDRARANLRAVKQVRARLQHGDGHLGLPEAAPFDAIIMTA
                     AASHVPPALLEQLLPGGRMILPLGTQEQFLWMIDKQEDGSIQKTRLDPVRFVPLLNGQ
                     Q"
     gene            complement(4296..5042)
                     /gene="surE"
                     /locus_tag="PKN55_04145"
     CDS             complement(4296..5042)
                     /gene="surE"
                     /locus_tag="PKN55_04145"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012695845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HNX70466.1"
                     /translation="MNFLISNDDGYFAPGLAALAQTLSRLGNVTVVAPERDRSGASNS
                     LTLDRPLTARRAANGFFSVNGTPTDCVHLAITGLMDSPPDMVFSGINHGPNMGDDTLY
                     SGTVAAATEGYLLGIPSVAMSLAGKPGEHFASACLVAEQLVRRWQEAPWAQGAVLLNV
                     NVPDVPPEQLGPWQVTRLGRRHHAEPVVQSRNPRGETIYWVGAVGRAQDAGPGTDFHT
                     VAAKGVSVTPLQLDLTAHHTLDPLSAWLAP"
     gene            complement(5126..6043)
                     /gene="nadA"
                     /locus_tag="PKN55_04150"
     CDS             complement(5126..6043)
                     /gene="nadA"
                     /locus_tag="PKN55_04150"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011137225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HNX70467.1"
                     /translation="MSPRVVTLAHYYTAPEIQRMADKVGDSLELSLYARDAQADIIVF
                     AGVRFMAETAKILNPQAQVILPDAGSTCSLVTQTDVAALRAWRESYPDHVHVSYINSS
                     AEHKALSDWIVTSRNVDDIIAHLYAEGKKVIFSPDRNMGAYLNFQYGYDMPLWSAVCE
                     VHDKFNEDALNEAFAGVSGQAFLIAHPESPLPVLQRADYVGSTSGMLNWVKSYQGELS
                     ATIFVATEDGILYNMHEARPDLNLVQAPIYAGCQCNSCPYMKLNTVAAVQAAQAGQGE
                     RIDYLTPEQMDAARLPIERMLAFSQRYVA"
     gene            6612..9284
                     /locus_tag="PKN55_04155"
     CDS             6612..9284
                     /locus_tag="PKN55_04155"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011137223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetate--CoA ligase family
                     protein/GNAT family N-acetyltransferase"
                     /protein_id="HNX70468.1"
                     /translation="MKPHYLTPLFAPRTVAVIGASDTPGSVGQAVFANLLAGGYQGKL
                     YPVNLNHRVVGGIAAVPNVKMLEPEIDLAVVTTAIRSLPGVMKDCAKKGVKAVLLTRE
                     FADTDELESEVMKESLAIAKRAGVRVLGPNMLGLMRPVIGLNASNYSAKVRPGNLALV
                     AHSSALCTAMLDWAEAKGIGFSSVISMGESSDLDFGEILDYLVADGYTQGILLHVHSI
                     HDARRFMSSLRAAARGKPVVVIKSGRYLDQHTGLTHSSHMVASGDVFDSALARAGVLR
                     VNTIAQLFTAARVLAAKYRVQGTRLAIVTNGIGPGVLAADSVMSYGAELAQLSPDTMQ
                     LLNEELPRNWSRANPVDIIGDASPVRFRMAVKACLDDPNVDGVLVIFTPQAGTDHHTT
                     AELMVGLQKETTKPMFMSWLGDSKVASSRELFSRARMAHFRTPEFAVEVFRSLASFHY
                     NQQLLLQTPGPLQGEQAEPNVAHAQSIIDAALAQGREVLSERESKEILAAFNIPVNQT
                     RLATTADEAVAHAETLGYPVVLKIDSPDVIYKSDVGGVELNITNSASVREVFANILER
                     TRQALPNALVHGVSVQPMVRRRHARELMIGVVHDNSFGPVITFGAGGIATEIQHDRSI
                     ALPPLNSYLAEKMIARTRISKTLGEFKHMPAVDMDQLKTLMGRVSDMVCELPDLLEAD
                     INPLLIDDRNAVVLDARIIVRKTRHSRRYRHMAIMPYPSDMVEHMTLNDNTEVTIRPM
                     RPEDADMQQEFVRNLSEESRYNRYMSSIKQLSQPVLVRFTQLDFDREMALVMTRMVDG
                     REEQLGVSRYFTDPDNESCEFALVVADNWQGRGIGPIMMNRLFDAAKRQGLTIMRGEV
                     LANNKGMLKLMAKLGFTVSPHPEDRQLTWVTRPL"
     gene            9453..9701
                     /gene="rpsP"
                     /locus_tag="PKN55_04160"
     CDS             9453..9701
                     /gene="rpsP"
                     /locus_tag="PKN55_04160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003740833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HNX70469.1"
                     /translation="MVVIRLARGGAKNRPFFNIVVTDSRNRRDGRFIERVGFYNPVAN
                     ERQERVRLAMDRVNYWTGVGAQVTEAVAKLLKQQKVAA"
     gene            9704..10252
                     /gene="rimM"
                     /locus_tag="PKN55_04165"
     CDS             9704..10252
                     /gene="rimM"
                     /locus_tag="PKN55_04165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007343854.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HNX70470.1"
                     /translation="MSGSPVMTGAAAPDESTLVSMGYIAGAFGVRGWVKIHADTEYAD
                     SLFDYPRWWVGRPGGAWRAYTLEDGSVHSKGVAAKLEGVLDRDQAALMRGLTIAIERA
                     ELPPPEEGEYYWTDLIGLSVVNTQGETLGVVSELLATGAANDVLVVRQDKTERLIPFV
                     GAYVLEVDLPGKTIRVDWGLDY"
     gene            10255..11010
                     /gene="trmD"
                     /locus_tag="PKN55_04170"
     CDS             10255..11010
                     /gene="trmD"
                     /locus_tag="PKN55_04170"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014085998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HNX70471.1"
                     /translation="MQIDVVTLFPEMFSALTASGVTRRALEQGIWALHTHYPRDFAYD
                     NYRTVDDRPYGGGPGMVMRAEPLEAAIIAARQRQAEAGVADSHCIYLSPQGRPLGDAR
                     VRELAQRPGLILLAGRYEGVDERLIQRQVDEEISIGDYVLSGGELPAMVLMDAVLRLL
                     PGVLNHAQSAEQDSFVDGLLDCPHYTRPEEYQGVRVPDVLLSGDHARIARWRLKQSLG
                     RTASRRPDLLAARPLSKQESRLLAEYQQEQDSA"
     gene            11027..11398
                     /gene="rplS"
                     /locus_tag="PKN55_04175"
     CDS             11027..11398
                     /gene="rplS"
                     /locus_tag="PKN55_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003798459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HNX70472.1"
                     /translation="MNLLQQLEQEEIARLGKTIPDFAPGDTVIVQVKVKEGNRERLQA
                     YEGVVIAKKNRGLNSAFTVRKISAGTGVERTFQTYSPLVAQIDVKRRGDVRRAKLYFL
                     RERSGKSARIKEKLPAKKTAA"
     gene            11496..>11962
                     /locus_tag="PKN55_04180"
     CDS             11496..>11962
                     /locus_tag="PKN55_04180"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011137218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HNX70473.1"
                     /translation="MATDSSRSPDESAVLAAPFGRLGVAAHAGVLTGIAFLPADTPLR
                     APISDGVCAQLAQELASYWDAPQHVFQLPWRAEGTLFQQRVWQVMLQIPPGQTLSYGQ
                     VAQQLASAPRAVGGACGRNPLPIVIPCHRIVAAGGLGGFNQGRDEGLLGVKRW"
BASE COUNT         2216 a         3725 c         3755 g         2266 t
ORIGIN      
        1 acggtgggcg tcaaacccgg cagagatgaa gatcatctgc ggctggaaag cttccagatt
       61 gggcagccag gccatttcca ctgcttcgcg gaaggcggag ctgccgctgc cggccttcat
      121 cggcacattg tgcatgttcg ggcccagcgg cacgtcgcca cagtaggggt agaacgggtg
      181 ctggaagatc gacaccatca tcacgcgcgg gtcgttctgg aagatttctt cggtgccgtt
      241 gccgtggtgc acgtcaaagt cgatgatggc cacacgctcc aggccgtgct gcgacagcgc
      301 gtgagaggcg gcgatggcca cgttgttgta aaaacagaaa cccatcgact tggccgattc
      361 ggcgtggtgg ccaggcgggc ggatgctgca aaaggcgttg ggggccaggc cttcgcatac
      421 catgtccact gccttgaccg cgccgcccgc cgcgcggcgc gcggcgcgca gggtgtgcgc
      481 catcatggcg gtatcaggat ccacgcgata ggtgccgctg gccggcgtgc tggcttccag
      541 cgattccagg tagtgcggcg gatgcacgcg ggcgagttgc tggtcggtga cttccggcgc
      601 ctcgacttcg tgcagttcgt cgtacagttg cgaggccatc agttggtcac ggatggcaat
      661 cagccgctcg gggcattccg ggtggccttc gcccacattg tgcagcaggc aatcctggtg
      721 ggtgatgaag gcggtcagac cggaactggg gagttgtttt ttaatccacg tcaacaattg
      781 actgcttcct gatagttcgt ccatttgtag aaagggccac gacttccccg tgcacgccct
      841 ggccagcggt cgcgtccgca cgccagcgcc cgcccggaaa gggaacaggg agcgacatca
      901 acgaaacgcc agcggatgac agcgtcgtca gcctttccga ctgcgcgctg ttcggtttat
      961 caccttgatc ctgcgcagaa aacccggagg gctgcacggt caggcttatg atttttggat
     1021 gaatccaagt gtgcgctgaa tgtcgaaaaa atgcaatcgt tcagcccggg attgcgccag
     1081 gccagatgcg ccacgccccg ccgggcggaa aagccgggcg gggcgtggca aagtgaggca
     1141 caacagcaga tcagagcagc atgtctttgg agacgccctt gcgccgcaac agccggcgca
     1201 gggtttccag gccttcgatc tggatttgcc gcacccgctc gcgggtcagg ctcatgctgg
     1261 cggccagttc ttccagcgtg cagatttcgt agccattcaa gccatagcgg cgctcgatca
     1321 ctgtgcgttg cttgtcatta agctgttgca gccactcgcg cacatagcgc tcgatttcgg
     1381 cgttgtgcag cagctcatct ggctcttcct gctgctcgtc ggggatggat tcacccacag
     1441 tcagcatcgg gtcgatgtcc agcggcgcat ccagcgaggc cacccgttcg ttcaggctga
     1501 ggatgcggcg gacttcttcc actggcttat ccaccagatg ggagatttcc tccagcgtcg
     1561 gctcgcgccc ggcctgcatt tccagatggc gctgggcgcg cagacagacg ttcagctctt
     1621 tgatcacatg aacaggcagg cgaatggtgc gcgactggtt catgatcgcc cgctcgatgc
     1681 tctgccgaat ccaccaggta gcgtaggtgg aaaagcggaa cccgcgttcg gggtcgaatt
     1741 tttccagcgc gtgcatcagg ccgatattgc cttcctcgat caggtcaagc agcggcaggc
     1801 cccggttgat gtagtgcttg gcgatgttca ccaccaggcg caaattgtgc tcaatcatgc
     1861 gctgacgcgc agcgaaatcg ccttgtacca cgcggcggca cagggctttt tcttcgtcgg
     1921 cgctcaagag cgcattgtgg ccgatgtcgt tcagataaat ctgggtgaca tcggcgagct
     1981 cgtccatgct ggcgaacgag gaatcggcaa cgtcctcgct ggtgtcggcg tcagcgacgg
     2041 cctcctccag cggttcttcc agatccgaca cttcttcgtc atggaggctg tcgatactgt
     2101 ggctcatgct atgtcccctt gctccccatg ctgcccgcca gcgacacacg ctggcatgga
     2161 tcagggagcg ttacccagat atttttgcgg gtcaacaggc ttgccgaagc gccggatctc
     2221 gaagtgcagc ttgacctgat cggcgtcgga attacccatc tccgcaattt tttgcccctt
     2281 ggtgacgact tggccttcct tgaccagcaa ctggctgtta tgcgcgtagg cggtcaggaa
     2341 agtcttgtta tgcttgatga tgatcagctt gccgtagcca cgcaggcccg aaccactgta
     2401 caccacccgg cctttggccg aggccagcac cggctggccg gttttgccgc cgatgtcgat
     2461 acctttattg ttgtcggtga agcggttgat caccttaccc tgcgttggcc aaccccaact
     2521 ggccacatct tcttcacctg cctgggcgct ggcggccggc tctgcaacgg tcggcgcacg
     2581 aggcgcttca gtattcttgc tgctgtcgcg ttctttgtcc ttgcccagct tgtctttgtc
     2641 gacggctttg ctatcgactt tattatcttt ctcggttttg ctggccggct tgtctgcctt
     2701 ggcctgtttt tcggtcttgc tgtcgctacg tgcctcgctc ttgtccgcgg atttgtctgc
     2761 tggcgcggct tctgccaccg agccaatctt gcgcacggct tcagccgaat acggcagctt
     2821 gaccgccttg gggccatgac gagcgccggc atcagccacc ggcggggtag gcgccggttt
     2881 ttctgcggcc ggaaccgcac caggcgccac cgccacactg cgtgcaggcg caggctcggc
     2941 aggcggggtc agccgcaaca cctgatctac cttgatgacc cccgggtcat cgagttggtt
     3001 ccaggcagca atatcacggt aatcctggcc gtagttcagg gcaatactgt acaacgtgtc
     3061 gccagccttg accttgtagc tgtccggtcg gtcatcgtcc ggatcgcggc tggaaggcgc
     3121 ccgcgagccc attggcacgg gcgtcggcgc aggcgcggct tgcgacgaat tcattggata
     3181 ggcgggaata tgcgtactgc tggccgtagt cgtggtgctg gtgctgctgg ccggagagac
     3241 cttcaccggg gctttggcac gatacacgct gacctcaccc gctggtgcgg cctgcaccgt
     3301 caccgcgccc cggctgccgc tggccatcgg cgcaggcggc ggagtgtagg catcagggta
     3361 ggccgtggcc ggacgggtct gtccgccctg cacaaccggg gcagggcttt gtgacagcgc
     3421 agaacatccg gacagggtca ggccaagcac ggcaaggtag aacggtttag cggctcgcat
     3481 agttcactat cattgagtca tttgtaagac gaccccatcc gtttgtctag cgacttcggc
     3541 aaggattcgg cggattttct ttgaaacggc attcaacagc ccggacgcat gtcgcatgca
     3601 tacaacatgc gtacaaaatg gcatgccata ccggccggct tattgctgac cattcagcag
     3661 tggcacaaaa cgcacagggt caaggcgggt tttctggatg gagccatcct cttgtttgtc
     3721 aatcatccag agaaactgct cctgggtgcc cagcggcaga atcatgcgcc cgccaggcag
     3781 taattgttcc agcagtgccg gcggcacatg gctggccgcc gccgtcatga tgatggcgtc
     3841 aaaaggggcg gcttccggca ggcccagatg gccgtcacca tgttgcagcc gcgcacgcac
     3901 ctgcttgacg gcgcgcagat tggcgcgggc acgatccagc agggcggcga tgcgttcgat
     3961 ggaatacacc tcgggaaata atttggtcag tacggcggtt tgatacccac aaccggtgcc
     4021 gatttccaga atcttgcgcg gcgatttgcc attgagcagc agttcggtca tccgcgccac
     4081 ggtaaacggc tgggaaatgg tctgaccgtg gccgatgggc aaagcgtcgt cgtcataagc
     4141 gcgatgcgcc agcgcgtttt ccacgaacag gtgacgtggc aattctgcca tcgccgccag
     4201 cacgcgggcg tcggcaatgc ctttttcccg cagccggtca accatccggg tacgggtgcg
     4261 cggtgatgtc atgccaatgc caagttgcgg ggtgttcatg gggccagcca agcagaaaga
     4321 gggtcaagag tgtgatgggc ggtgagatcc agttgcaaag gtgtcaccga cacacctttg
     4381 gccgccacgg tatgaaaatc ggttcccggg ccggcatctt gagcacgacc gacagcaccc
     4441 acccagtaaa tggtttcacc acgcggattg cgactttgca ccaccggctc ggcatggtgg
     4501 cgacggccca gacgggtcac ctgccatggg cccagttgct cgggtggcac atcgggcacg
     4561 ttgacgttca gcagcaccgc cccctgcgcc catggcgcct cttgccagcg gcgcaccagt
     4621 tgctcggcca ccaggcaggc gctggcaaag tgttcgccgg gcttgccggc cagcgacatg
     4681 gctaccgagg gaatgccgag cagataacct tcggtggcgg cggccaccgt gccggaatac
     4741 aaagtatcgt cgcccatgtt cgggccgtgg ttgatgccgg aaaacaccat gtccggcggg
     4801 ctgtccatca ggccggtgat ggccagatgc acgcaatcgg tgggcgtacc gttgacgctg
     4861 aaaaaaccat tcgccgcccg acgtgccgtc agcggccgat ccagggtcag cgaattggag
     4921 gcgccgctgc ggtcgcgctc cggcgccacc acggtgacgt tgcccaggcg tgacaaggtc
     4981 tgcgccagcg cggcaaggcc gggggcaaag taaccatcgt cattgctgat gagaaaattc
     5041 atggcgagtc acaaaaggca atgccgcgat tgtaacccgc cacgggtaaa agacaatcgt
     5101 cattcacgtg gcgggcggaa tgccttcagg cgacgtaacg ttgactgaac gccagcatgc
     5161 gctcgattgg caggcgggca gcgtccatct gttccggtgt cagatagtcg atacgctcgc
     5221 cctgaccagc ctgcgccgcc tgcacggcag ccaccgtatt cagcttcatg tacggacagg
     5281 aattgcactg gcagccagcg tagatcggcg cctgtaccag gttcagatcc ggtcgcgcct
     5341 catgcatgtt gtaaaggatg ccatcttcgg tggccacaaa aatcgtcgcc gacagctcac
     5401 cttgatagct cttgacccag ttcaacatac ccgaggtcga tccgacgtaa tctgcccgtt
     5461 gcagcaccgg tagcgggctt tccgggtgtg caatcaggaa ggcctggccg ctgacgccgg
     5521 caaacgcctc gttcagcgcg tcttcgttga acttgtcgtg cacttcgcac accgccgacc
     5581 acagcggcat gtcgtagccg tactggaagt tcaggtaggc gcccatattg cggtcgggcg
     5641 agaagatcac ctttttgcct tcggcgtaca gatgggcaat gatgtcatcg acattgcggc
     5701 tggtgacgat ccagtcggac agggccttgt gttcggcgct ggaattgata tacgacacgt
     5761 gcacatggtc cggatagctt tcacgccagg cgcgcagcgc ggcaacatcc gtctgggtaa
     5821 ccagcgagca ggtcgaaccg gcgtcgggca ggatgacttg cgcttgcggg ttgagaatct
     5881 tggcggtttc tgccatgaag cgcacaccgg caaacacaat gatgtcagcc tgggcatcac
     5941 gcgcgtacag cgaaagctcc aggctgtcgc ccaccttgtc agccatgcgc tggatttcgg
     6001 gtgcagtgta gtaatgggcg agggttacaa cacgggggga catcgggtta tggctcctgc
     6061 cgcactgacg cggcgatatg ggctggcaaa gtcgcggcga catgccctgc gccggaaccc
     6121 ggcgatgccg cgcttgaccg cagccggcca gcctttgcgc aaggtgcgca gaccaggctg
     6181 gcggtgcaat gcatcatgaa aaacaatcac gccaatcccc cagggcggga tggacggcgc
     6241 ttgcgaatag tggaacatag caaaaaagcc acagcgctgc cagccacgtg cgcattggcg
     6301 atgttaatga accacggttt tttgcgaagg gaaacagtct ggtagataat gctgcaccgc
     6361 acaatcatat tttgacatgg cacttccacg acgccggcca tcgccgggac tggcgtcacc
     6421 atggcggtgc ggcgcatttt gcggcagcct tgatgttgcg gcgcaccgcg cccacgcccc
     6481 gcactcaccc cccacccggc gatggcaagc acgcgacagg acgctttcat ccccccgcct
     6541 gtccggccct gtttgcggcg cgcgtcaccc gcgcgtctcg ataaaactga ttgaatgacc
     6601 aggttaatat catgaaaccg cactatctca ccccgctttt cgcgccccgc accgtcgccg
     6661 tcatcggcgc cagcgacacc ccaggctctg tcggtcaggc ggtgttcgcc aacctgctcg
     6721 ccggggggta tcagggcaag ctgtatccgg tcaacctgaa tcaccgcgtg gtgggcggca
     6781 tcgccgccgt gcccaatgtc aaaatgctgg agccggaaat cgacctggcc gtggtcacca
     6841 ccgccatccg ttcgctgccc ggcgtgatga aggattgcgc caaaaaaggc gtcaaggccg
     6901 tgctgctcac ccgcgaattc gccgacaccg acgagctgga aagcgaggtg atgaaagaat
     6961 cgctggccat cgccaagcgc gccggcgtgc gcgtgctggg cccgaacatg ctgggcctga
     7021 tgcgcccggt gatcggcctg aacgccagca attattccgc caaggtgcgc ccgggcaatc
     7081 tggcgctggt ggcgcattcg tcggcgctgt gtaccgccat gctggactgg gccgaagcca
     7141 agggcattgg tttttccagc gtgatctcga tgggcgaaag ctcggacctg gactttggcg
     7201 aaatcctgga ttatctggtg gccgacggct acacccaggg gattttgctg catgtgcact
     7261 ccatccacga cgcccgccgc ttcatgagct cgctgcgcgc cgccgcgcgc ggcaagccag
     7321 tggtggtgat caaatccggc cgctatctgg accagcacac cggcctgacc cactccagcc
     7381 atatggtggc cagcggcgat gtgtttgact ccgcgctggc gcgcgccggt gtgctgcggg
     7441 tgaacaccat tgcccagctg tttaccgccg cccgcgtgct ggccgccaaa taccgcgtgc
     7501 aaggcacccg gctggcgatt gtcaccaacg gcatcggccc cggcgtgctg gccgctgaca
     7561 gcgtgatgag ctacggcgcc gagctggccc agctgtcgcc ggacaccatg cagctgctga
     7621 atgaagaact gccgcgcaac tggtcgcgcg ccaacccggt ggacatcatc ggcgacgcca
     7681 gcccggtgcg ctttcgcatg gcggtcaaag cctgtctgga cgacccgaac gtggacggcg
     7741 tgctggtgat cttcaccccg caggccggca ccgaccacca caccaccgcc gagctgatgg
     7801 tgggcctgca aaaagaaacc accaagccga tgttcatgtc ctggctgggc gattcgaaag
     7861 tggcctccag ccgcgagctg ttcagccgcg cgcgcatggc gcacttccgc accccggaat
     7921 ttgccgtgga agtgttccgt tcgctggcct cgttccacta taaccagcaa ctgctgctgc
     7981 aaacccccgg ccccttgcag ggcgaacagg ccgagccgaa tgtggcgcac gcgcaaagca
     8041 tcatcgacgc cgcgctggcc cagggccggg aagtgctgtc cgagcgcgaa tccaaagaaa
     8101 tcctcgccgc cttcaatatc ccggtcaacc agacccggct ggccaccacg gctgacgaag
     8161 ccgtggcgca cgccgaaacg ctgggctacc cggtggtgct gaagatcgac tcgccggacg
     8221 tgatttacaa atccgacgtc ggcggcgtcg agctgaacat caccaacagc gcctcggtgc
     8281 gcgaagtgtt cgccaatatt ctcgagcgca cccgccaggc gctgcccaat gcgctggtgc
     8341 acggcgtgtc ggtgcaaccg atggtgcgcc gccgccatgc ccgcgagctg atgatcggcg
     8401 tggtgcacga caactccttt ggcccggtga tcacctttgg cgcgggcggc atcgccaccg
     8461 aaatccagca cgaccgcagc attgccctgc cgccgctgaa cagctatctg gccgaaaaaa
     8521 tgatcgcccg cacccgcatc agcaaaacgc tgggcgagtt caagcacatg ccggcagtgg
     8581 acatggacca gctgaaaacg ctgatgggcc gtgtgtccga catggtgtgc gagctgcccg
     8641 acctgctgga agccgacatc aacccgctgc tgatcgacga ccgcaacgcc gtggtgctgg
     8701 acgcgcgcat catcgtgcgc aaaacccgcc attcgcgccg ctatcgccat atggccatca
     8761 tgccctaccc gtcggacatg gtggagcaca tgacgctgaa cgacaacacc gaggtcacca
     8821 tccggccgat gcgcccggaa gacgccgaca tgcagcagga attcgtgcgc aatctgtcgg
     8881 aagaaagccg ctacaaccgc tatatgtcca gcatcaagca gctgtcgcag ccggtgctgg
     8941 tgcgcttcac ccagctggac ttcgaccgcg aaatggcgct ggtgatgacc cgcatggtcg
     9001 atggccgcga agagcaactg ggcgtgtcgc gctactttac cgacccggac aacgaatcct
     9061 gcgaattcgc cctggtggtg gccgacaact ggcaagggcg cggcatcggc ccgatcatga
     9121 tgaatcgcct gtttgacgcc gccaagcgcc aggggctgac catcatgcgc ggcgaagtgc
     9181 tggccaataa caagggcatg ctcaagctga tggccaagct gggctttacc gtctcgccgc
     9241 atccggaaga tcgtcagctc acctgggtga cccgtccgct gtaaactgcc tgtctgcgcg
     9301 ccgggccaag cgcaggtggc tggcccggcg cgcggctttt gtgaccctgg gcgcgacacc
     9361 ctgcgcccag ggttgtttaa acacttgcca gtaaaaggaa aagcggctta aaatcgcagc
     9421 ctttttactg tctgattaaa ctaggaacca ccatggttgt gattcgtctt gctcgcggcg
     9481 gcgccaagaa ccgtccgttt ttcaatatcg tcgtgaccga ttcccgcaac cgccgcgacg
     9541 gccgcttcat tgagcgcgtg ggcttctaca acccggtcgc caacgagcgt caagagcgcg
     9601 tgcgtctggc gatggaccgc gtgaactact ggaccggcgt cggcgcgcaa gtgaccgaag
     9661 ctgtggccaa gctgctcaag caacagaaag tggccgcctg agcatgtcgg gctcccctgt
     9721 gatgaccggg gccgctgcgc ccgatgaatc gacgctggtc agcatggggt atatcgccgg
     9781 cgccttcggt gtgcgcggct gggtgaaaat ccacgccgac accgaatacg ccgacagcct
     9841 gttcgactac ccgcgctggt gggtgggtcg tccgggcggc gcctggcgcg cctacacgct
     9901 ggaagatggc tcggtgcaca gcaaaggcgt ggccgccaag ctcgaaggcg tgctggatcg
     9961 tgaccaggct gcgctgatgc gcgggctgac catcgccatc gaacgcgccg aactgccgcc
    10021 gccggaagaa ggcgagtact actggaccga cctgatcggc ctgagcgtgg tcaataccca
    10081 gggcgaaacc ctgggcgtgg tgagcgaact gctggccacc ggcgccgcca acgatgtgtt
    10141 ggtggtgcgt caggacaaga ccgaacggct gattccgttt gtcggcgcct acgtgctgga
    10201 agtcgatctg cctggcaaga ccatccgggt ggactggggg ctggactact gacgatgcag
    10261 atcgacgtcg tcaccctgtt cccggagatg ttcagcgcgc tgaccgccag cggcgtcacc
    10321 cgacgcgcgc tggagcaagg catctgggcg ctgcacaccc attacccccg ggatttcgcc
    10381 tacgacaatt accgcaccgt ggacgatcgc ccttatggcg gcggccccgg catggtgatg
    10441 cgggcggaac cgctggaagc ggcgattatt gccgcgcgtc agcgtcaggc tgaggccggc
    10501 gtggccgaca gccattgcat ctacctgtcg ccgcaaggcc gcccactggg cgacgcccgc
    10561 gtgcgcgaac tggcgcaacg ccctggcctg atcttgctgg ccgggcgcta cgaaggggtg
    10621 gatgagcggc tgatccagcg tcaggtcgat gaagaaatct ccattggcga ctatgtcctg
    10681 tccggcggcg agctgccggc catggtgctg atggatgccg tgctccggct gttgccgggt
    10741 gtgttgaatc acgcccagtc ggccgaacag gattcgtttg tggacgggct gcttgactgc
    10801 ccgcactaca cccggcctga agaataccag ggggtgcgcg tgccggatgt cttgctgtcc
    10861 ggcgaccacg cccgcattgc ccgctggcga ctcaagcagt cgctggggcg cacggccagc
    10921 cgtcgccctg atctgcttgc cgctcgcccg ctatccaaac aggaatctcg gctgctggct
    10981 gagtatcagc aggaacaaga ttccgcataa caactggagt aatcccatga atctgctgca
    11041 acaactcgag caggaagaaa tcgcccgcct gggcaaaacc atccctgact tcgcaccggg
    11101 tgacaccgtg atcgtgcaag tgaaggtgaa ggaaggcaac cgtgagcgtc tgcaggctta
    11161 cgaaggtgtg gtgatcgcca agaaaaaccg tggcctgaac agcgccttca ccgtgcgcaa
    11221 aatctccgcc ggcaccggcg tggaacgcac cttccaaacc tactcgccgc tggttgccca
    11281 gatcgacgtg aagcgtcgcg gtgacgtgcg tcgcgccaag ctgtacttcc tgcgcgaacg
    11341 ctcgggcaag tccgctcgca tcaaggaaaa gctgccggcc aagaagaccg ccgcttaatc
    11401 gcgccgggtt cttcgccaca gcaaaacacg ctggccatct ggtcagcgtg ttttttttgc
    11461 gccatgctgc cgcttttgcc ccagaaagcc ccgccatggc caccgactct tcgcgctctc
    11521 ctgatgaatc cgccgtgctg gcggcgccgt ttggccggct gggcgtggcc gcgcacgcgg
    11581 gcgtgctgac cggcattgcg tttttgccag cggacacccc cttgcgcgcg ccgatcagcg
    11641 atggcgtctg cgcgcagctg gcgcaagagc tggccagcta ctgggatgcg ccgcagcacg
    11701 tcttccagct gccctggcgc gccgagggca cgctgtttca gcagcgggtg tggcaggtga
    11761 tgttgcagat tccgccaggg cagaccctga gctatggcca ggtggcccag cagttggcca
    11821 gcgcgccgcg cgcagtgggt ggcgcgtgtg ggcgcaatcc cttgccgatt gtgattccgt
    11881 gtcaccggat tgtcgcagcg ggcggcctgg gcggattcaa tcagggacgg gacgaaggct
    11941 tattgggggt gaagcgctgg tt
//
LOCUS       DAOPYX010000051        11948 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_6028_length_11948_cov_11.335323, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000051 DAOPYX010000000
VERSION     DAOPYX010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 11948)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11948)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11948
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6028_length_11948_cov_11.335323"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..530)
                     /locus_tag="PKN55_04185"
     CDS             complement(<1..530)
                     /locus_tag="PKN55_04185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014086890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HNX70474.1"
                     /translation="MKVNLPVTQNEVFLDPARPIVTKTDLKGQITYANRAFIDISGFA
                     ENELIGVSHNIVRHPDMPPEAFADLWTTLKAGRPWSGLVKNRNKTGDFYWVEAYVSPI
                     TENGETIGYMSVRNAPKREDVAAAEALYRDVRDKRRTLPATPKGPGRYSLPLSKKLRS
                     AYWGAACLMLLIALLP"
     gene            complement(1062..1631)
                     /locus_tag="PKN55_04190"
     CDS             complement(1062..1631)
                     /locus_tag="PKN55_04190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018149998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HNX70475.1"
                     /translation="MTAKRIVVLAGDYVEDYEIMVPFQALQMLGHTVHAICPGKIAGE
                     QVRTAVHDFEGDQTYSEKPGHRFTLNASFAHCQPEQYDALLIPGGRAPEYLRLNPDVL
                     NWVRHFMQANKPVAAVCHGVQLLTAAGVLAGRKVTAYPACAPEVTAVGGEYVEVAVTD
                     AVTDGKLVTAPAWPAHPAWLAAWLTVLEA"
     gene            1829..2101
                     /locus_tag="PKN55_04195"
     CDS             1829..2101
                     /locus_tag="PKN55_04195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003101146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HNX70476.1"
                     /translation="MCELFSGQDPRRYTSVRRSLRLRGFVTSLSLEQYFWEVLDALSA
                     EQGMSTPQFIATLYDEVLRRHGEVRNFTSLLRVACGVHLQARSAPA"
     gene            complement(2171..2246)
                     /locus_tag="PKN55_04200"
     tRNA            complement(2171..2246)
                     /locus_tag="PKN55_04200"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2211..2213),aa:Phe,seq:gaa)
     gene            2953..3441
                     /locus_tag="PKN55_04205"
     CDS             2953..3441
                     /locus_tag="PKN55_04205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017509610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HNX70477.1"
                     /translation="MPHLDLDKTDIKILAALQQNGRLTNVELADAVALSPSPCLRRLK
                     HLEESGIIKRYVALLDPARLGLGLQAFVNVALEKRGSVHMQSFLQAVQRWPEVIACYA
                     LTGDMDYMLHVYCEDLDHFSRFVMGELLTHPGVEDLRSSFVLTDVKRTTALPLHQLGA
                     PR"
     gene            complement(3572..5080)
                     /locus_tag="PKN55_04210"
     CDS             complement(3572..5080)
                     /locus_tag="PKN55_04210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018746161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HNX70478.1"
                     /translation="MRRVLAMVSLLFTFLFVMPSWAEDAPAAAAAVTQAAPAADAPAA
                     APAAPAAAVAAAPAADAAAAPAPVANKGDTAWMLASTLLVLLMAAPGLGLFYGGLVRT
                     KNMLSILMQSLVVFCLLGVLWAVYGYSLAFTEGNAFFGSLDKLFLKGVTVDSMAATFS
                     KGVVIPEYVYIAFQLTFAGLTSALIIGGFAERIKFSAVLAFVVVWFTFSYLPLAHMVW
                     YWAGPDAYTTAEAGAKAGETAGFLFQKGALDFAGGTVVHINAGVAALVGALIIGKRVG
                     YGRESMAPHSLTMTMVGGAMLWVGWFGFNAGSNLEATGTAALAMVNTMLATCAATVSW
                     TLVEWMIKGKPSMLGAISGTIAGLVVITPACGFVGPMGSIIMGLLGGMVCYWGVNGLK
                     RMLGVDDSLDVFGVHGVGGILGALLTGVFAAPSLGGTGVYDYVANAVGEYDMVAQVTS
                     QAWGVGVAIIWSAVVSFVAFKLIDLTIGLRVSEEQEREGLDITTHGETAYHH"
     gene            complement(5138..5476)
                     /locus_tag="PKN55_04215"
     CDS             complement(5138..5476)
                     /locus_tag="PKN55_04215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HNX70479.1"
                     /translation="MKWISAIIKPFKLDEVRVALEDIGVRGLTVTEVKGFGRQKGHTE
                     IYRGAEYQVSFLPKTRIDIAVRNDQLDAALAAIEKAAATGKMGDGKIFVLDLQQVIRI
                     RTGESGEDAL"
     gene            6013..6978
                     /gene="speB"
                     /locus_tag="PKN55_04220"
     CDS             6013..6978
                     /gene="speB"
                     /locus_tag="PKN55_04220"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009116223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HNX70480.1"
                     /translation="MSEMIYGDGAIRRPGLYGSSIENTYAGVLSFMRRTYSRELNGVD
                     VVVSGVPLDLSTTFRSGARLGPQAIRAASVQLAELKPYPWGFDPFDDLAVIDYGDCWF
                     DAHNPLSIKPTIVEHARTILASGAKMLTLGGDHFITYPLLIAHAEKYGKPLALLHFDA
                     HCDTWPDDSPDSLNHGTMFYKAIKDGLIDPAKSVQVGIRTWNDDFMGVKVLDAPWVHD
                     HGPEAVLAEITRVIGDHPVYVTFDIDCLDPSAAPGTGTPVPGGLSSAQALKIVRNLGG
                     LNIVGMDVVEVAPSYDQSEITAIAAAHIACDMLCLLRNQKLARQG"
     gene            complement(7147..7222)
                     /locus_tag="PKN55_04225"
     tRNA            complement(7147..7222)
                     /locus_tag="PKN55_04225"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7187..7189),aa:Met,seq:cat)
     gene            complement(7301..8122)
                     /locus_tag="PKN55_04230"
     CDS             complement(7301..8122)
                     /locus_tag="PKN55_04230"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014088359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HNX70481.1"
                     /translation="MDILLLCKALIMGIVEGITEFFPISSTGHLILAGDLINFDDGKG
                     KVFEVVIQLGAILAVCWEYRARIAALACGVRRDPVALRFVVGLGLAFLPAAVIGVLTI
                     KTIKFYLFNAVAVAVALVVGGLVILWVESRPQKPRITRIDDMRLRDALKVGFAQVFSL
                     IPGVSRSGSTIIGGMLFGLDRKVATEFSFFLAIPIMFAATAYDVLKHWQAFSMADVPV
                     FGVGFVAAFLSAFLAVRGLLRFVATHTFVVFAWYRIVFGLLILLSWKMGWVSWTA"
     gene            complement(8249..8863)
                     /locus_tag="PKN55_04235"
     CDS             complement(8249..8863)
                     /locus_tag="PKN55_04235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014088360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HNX70482.1"
                     /translation="MMLKKWWVALLLLVSAVAMADIQPGRDYVELRAPQPSGAPAGKV
                     EVIEFFSYTCIHCYRLEPAVESWAAKLPAHVSFRREQIVWSKEMEPLARLFATQRQMG
                     LLDKLHKPVFDAMMRDKQNLADEKLLTPFLKAQGVDVNAFLQTSKSFGMASQVSRAAK
                     MTRDYQVEGTPTFVVAGRYATMAAEPARLLQVVDELIAKAKNGR"
     gene            complement(9105..9989)
                     /locus_tag="PKN55_04240"
     CDS             complement(9105..9989)
                     /locus_tag="PKN55_04240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011137546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HNX70483.1"
                     /translation="MSRDYKNSTRPPRGGNGASRGAPAPRTPASTSAKKSSGNGKGVV
                     AGVLIGLLVGVGGAAGVAFYLNRANTPFAPRDAKPAKEEASDMVSLPAQPARPAPPPG
                     PATLEPGAPGSRAPDLAANLPTNPLLDTPPASPPPAAPPAAAPQPSQAPAEDKPARPG
                     KADAKPDAPQTPAEKHAKAEPPQYDFYKILPGNAPPPAPQEKPATDPAKDKEAADKGK
                     KIYLQVGAYQKEVDADNMKAKLALLGVEANIQSVNSSDKGLLHRVRIGPLTRSEDVER
                     LRGQLKANGLNPTPVKAD"
     gene            complement(10139..11629)
                     /locus_tag="PKN55_04245"
     CDS             complement(10139..11629)
                     /locus_tag="PKN55_04245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018152652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HNX70484.1"
                     /translation="MSLARITSRALAGTDAPEVTVEVHLANGLPSLTLVGLPDTEVKE
                     SRERVRAAIQLAGFEFPNRRVTINLAPADLPKDSGRFDLPIALGILAASGQLSAQHLA
                     RHEFVGELALNGELRPIRGALLMAMAATRAGRTLVLPRASAGEAALWPQASLAQADSL
                     QAVCAMLNGQARLEAPEPPPPALAPNYPDLSDLKGQLAARRALEIAAAGRHSLLLSGP
                     PGTGKSMLATRLPGILPAMSETEALEVASVRSLASGGLDPTQFWTRPYRAPHHSASAA
                     ALVGGGGQPRPGEVSLALHGVLFLDELPEFERKTLDMLREPLETGRVTISRAARQVDF
                     PARFQLVAAMNPCPCGYHGHPSGRCRCTPEQISRYRGKISGPLLDRIDMRVEVPSLPA
                     QDLLRAAPGEPSARVRERVEAAFAIQLARQGVANALLDGATLDDIARPDAEGSALAAQ
                     AIERFHLSGRAYHRILRLARTIADLEASAAVNARHIAEAIQYRRGL"
     gene            complement(11641..11946)
                     /locus_tag="PKN55_04250"
     CDS             complement(11641..11946)
                     /locus_tag="PKN55_04250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011137548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory factor UbiK family protein"
                     /protein_id="HNX70485.1"
                     /translation="MSQKLFDEISAKIGDTLASSPAKDLEKNLRSLMGSAFTKLDLVT
                     REEFDVQQEVLARTREKLEQLEARLAKLEALVLPAEPAADAAAAVEAEAEQAAQGHS"
BASE COUNT         2262 a         3713 c         3729 g         2244 t
ORIGIN      
        1 tccggcagca gggcgatcag cagcatcaga caggcggcgc cccagtaggc gctgcgcagc
       61 tttttgctca gcggcaggct gtagcggccg gggcctttgg gggtggctgg cagggtgcgg
      121 cgtttgtcgc gcacatcgcg gtacaaggcc tcggcggcgg cgacatcttc gcgcttgggg
      181 gcattgcgca ccgacatata gccaatggtt tcgccgtttt cagtgatcgg cgagacatac
      241 gcctccaccc agtaaaaatc accagttttg ttgcggttct tgaccaagcc gctccaggga
      301 cggccggcct tcagcgtggt ccacaggtcg gcaaatgcct cgggcggcat atcgggatgg
      361 cggacaatat tatggctgac gccaatcagt tcgttttctg caaagccact gatgtcgata
      421 aaagcgcggt tggcgtaggt gatctgcccc ttgaggtcag tcttggtcac gatcggccgc
      481 gccggatcga gaaagacttc gttctgggtg actggcagat tgactttcac acatgcctcc
      541 tgatcgccgc actcgggcag ggctgctgct gatgcggtct tgctgtctgg aaaggaacat
      601 ccactcggga tggtgtcttg ctgcctgagc atgacaatat tacaagaaag tgattatata
      661 ccggcttgcc ggtggattac tgttgaatat tgatatctat caacgacgtt cagcttgccc
      721 cgtgcatgtg atgacggcaa acgtttgttt tgccaggccc tagcagacca agcgtcatgc
      781 actgcacaca tgccaaatag aaggcccggc aagccacagt cagatctacg ctactctgat
      841 tgccgcgcca tcatgttgcg ctgggtcaaa accatgggca acaccaatga catgtaaacg
      901 cattgccccg gccaaatggc cggggcaatg cacaccgctc gccactccat cagatctaca
      961 ggggcttttt ctggtcatgg cgatgaccag gtgtctgacc gggatggatg aggcagtgat
     1021 gcacgcccag cgcaaagtgg ccacggcgtg cgactgctgg atcaggcctc gagcacggtc
     1081 agccaggctg ccagccaggc cggatgagcc ggccaggcag gggcagtgac cagcttgccg
     1141 tcggtcacgg cgtcagtaac ggcaacttcg acatattcac cccccacagc agtcacttcc
     1201 ggagcgcagg cggggtaggc ggtgaccttg cgtccggcca gtaccccggc ggcggtcagc
     1261 agttgcacgc catgacacac ggcggccacc ggcttattgg cctgcatgaa gtggcgcacc
     1321 cagttcagca catccgggtt cagtcgcaga tattccggcg cgcgaccacc aggaatcagc
     1381 agcgcatcgt attgctcggg ctggcagtgg gcaaagctgg cgttcaaggt gaagcggtga
     1441 ccgggttttt cgctgtaggt ctggtcgcct tcgaagtcgt gcaccgccgt gcgcacctgc
     1501 tcgccagcga tcttgcccgg gcagatggcg tgcacggtat ggccgagcat ttgcagtgcc
     1561 tggaacggca ccatgatttc gtaatcctcg acgtagtcac cagccagcac cacaatacgt
     1621 ttggcagtca tgcggaagtc tcccgtttga tgagtcatga gtctgggtca ctgctcacca
     1681 ccggccgtgc gctcgcatca gtcaagtaga ggtgcgcagc ccccaatgaa ggcggttacc
     1741 cgctgccttt cactgtagcc actcggtcgc gcaacctggt agtagcgatg tactacatga
     1801 gtatggattc agtcagaagg agaagtgcat gtgcgaattg ttttccgggc aggatccgcg
     1861 tcgttacacc agcgtgcgtc gttctttgcg gctgcgcggc tttgtcacca gcctgtcgct
     1921 ggaacagtat ttttgggaag tgctggatgc cttgtcggct gagcagggca tgagtacgcc
     1981 gcaattcatc gccacccttt acgacgaagt gctcaggcgg catggcgaag tgcgcaattt
     2041 cacgtcttta ttgcgggtgg cgtgcggggt acatctgcaa gcgcgcagcg cgccggcttg
     2101 aggtcagctt gcttcagacg ttcagacaaa acgaaaaccc ccgcatgcaa gcatgcgggg
     2161 gtttgaattc tggtggccag tcgcggaatc gaaccacgga cacgcggatt ttcaatccgc
     2221 tgctctacca actgagctaa ctggccctca agagatgcgt attaaaccgg atttcgccgg
     2281 atgggtcaag tggtttttgc aaaaaatctg catgcaacac aagtggagca gatttttgtt
     2341 ggtaaatcac acacttgctc gccagacgct gaaaaattgc gaatggggag cgttctgttt
     2401 gtcttgtggc gttggcgtaa gcagggaatt gcgcggcaat ggccaatttc ggccgcttgt
     2461 gcgcccagcc cgacaaacgc ggtggattcc cctgccaata tgctgactgt ccatattttc
     2521 cgcccccgtg cccggccatt ttctgtcaat cgtgcgttca attaggctga aagcaaattg
     2581 aaagcttggc tttttatgct tgcaaagcat tgaaactgaa caagtttctt gtgcggatat
     2641 ccgtcgattt tgggtgagcc aaggaggggg agtcctggct gttcgagata tgccaaaaaa
     2701 ttatggcgat gcattttctg ccaaaaactg cggccatcac gcaacagcca gcatgtttgg
     2761 ccagggtttt tccatgaatc atggccgatg actcgtagat tcacttaaaa ttcctgccag
     2821 gcaggaatga attttgcaag ctcgcccagt ccgcaggcgc ccattgccat tggcgccagg
     2881 ctcgacgcca agacaacaat gatatggccg attctgccgc cggccctgat acacactgga
     2941 gtaagcaata ccatgcctca cctcgacctg gataaaaccg atatcaaaat tctggccgcc
     3001 ctgcagcaaa atggccggct gaccaatgtg gagctggccg acgccgtggc gctgtcgccc
     3061 tcgccctgcc tgcgccggct gaagcatctg gaagagtcgg gcatcatcaa gcgttatgtc
     3121 gccctgctgg accccgcccg cctgggactg ggcttgcagg cgtttgtgaa tgtcgcgctg
     3181 gaaaagcgcg gcagcgtgca tatgcaaagc tttttgcagg cggtgcagcg ctggccggag
     3241 gtgattgcct gctatgcgct gacgggcgat atggattaca tgctgcatgt gtattgcgag
     3301 gatctggatc atttttcccg gtttgtcatg ggcgaattgc tcacccaccc cggcgtggaa
     3361 gacctgcgct ccagctttgt gctgaccgac gtcaagcgca cgacggcgct gcccttgcac
     3421 cagttgggcg cgccgcgctg agcggagctg gccaggcgcc cgcccgccgg attcgggaag
     3481 gcgcctggcc ggcaatagcc agaaaacaca aaaggcgccg caaggcgcct tttgtgttct
     3541 gtgccgcccg gcaaacgccg ggggttcagg atcagtgatg gtaggcggtt tcaccgtggg
     3601 tggtgatgtc cagaccttca cgttcttgct cttcgctgac gcgcagaccg atggtcaggt
     3661 cgatcagctt gaaggccacg aaggacacca cagccgacca gatgatggcc acgccaacac
     3721 cccaggcttg cgaggtcact tgcgccacca tgtcgtattc gcccacggcg ttggccacgt
     3781 agtcgtacac accggtgccg cccaggctcg gcgcagcaaa cacgccggtc agcagagcgc
     3841 ccaggatgcc gcccacgccg tgcacgccga acacatccag cgagtcatcc acgcccagca
     3901 tgcgcttgag gccgttcaca ccccagtagc acaccatgcc gcccagcaga cccatgatga
     3961 tgctgcccat cgggccgacg aagccgcaag ccggggtgat caccaccagg ccggcgatgg
     4021 tgccggaaat cgcgcccagc atggacggct tgcccttgat catccattcc accagggtcc
     4081 acgacacggt ggcggcgcag gtggccagca tggtgttgac catggccagt gcggcggtgc
     4141 cggtggcttc caggttggag ccggcgttaa agccaaacca gcccacccac agcatcgcgc
     4201 cgccaaccat ggtcatggtc aggctgtgcg gggccatgga ttcgcggccg tagcccacgc
     4261 gcttgccgat gatcagtgcg ccaaccaggg cagccacacc ggcgttgatg tgcaccacgg
     4321 tgccgccagc gaagtccagc gcgcccttct ggaacaggaa gcctgcggtc tcaccagcct
     4381 tggcgccagc ttcggccgtg gtgtaagcgt caggaccggc ccagtaccac accatgtgcg
     4441 ccagcggcag gtaggagaag gtgaaccaca ccaccacaaa ggccagcacg gcggagaact
     4501 tgatgcgctc ggcaaagccg ccgatgatca gcgcggaagt caggccggca aaggtcagct
     4561 ggaaggcgat gtacacgtat tccggaatca ccacgccctt gctgaaggtg gcggccatgg
     4621 aatccacggt cacgcccttc aggaacagct tgtccaggct gccaaagaag gcgttgccct
     4681 cggtgaaggc caggctatag ccatacaccg cccacagcac gcccagcagg cagaacacca
     4741 ccaggctttg catcaggatg gacagcatgt tcttggtgcg caccaggcca ccgtagaaca
     4801 gacccaggcc cggcgcggcc atcagcagca ccaagagggt ggaggccagc atccaggcgg
     4861 tgtcgccctt gttggcaacc ggagccgggg cagccgcggc atccgcagcc ggagcggcag
     4921 ccacagcggc cgcaggggct gccggcgctg cggccggggc gtcagccgcc ggcgcagctt
     4981 gagtcacagc ggcggcggct gccggcgcat cttccgccca gctgggcatc acgaacagaa
     5041 aggtgaacaa caagctcacc atggccagaa cacgtctcat gccactttct ccttaggttt
     5101 gaaaaacggc gagccgcccg gatgggccgg cgctcaatta cagggcatct tcgccggact
     5161 cgccggtgcg gatgcggatg acttgttgca aatccagaac aaaaatcttg ccgtcgccca
     5221 tcttgccggt ggcggccgct ttttcgatgg cggccagggc ggcgtccagt tgatcgttgc
     5281 gcacggcaat gtcgatgcgg gttttaggca aaaaactcac ctgatattcc gcgccacgat
     5341 agatttccgt gtggcccttc tggcgaccaa agcccttgac ctcggtgacg gtcagtccgc
     5401 gcacgccgat gtcctccagc gccacacgca cttcatcgag cttgaaaggc ttgatgatcg
     5461 cgctgatcca tttcatgttt cacctctgcg tttctcacgt gtttttgctg tgccggctgc
     5521 cgccctgcgc gggtgagtca gccgcttgtt tttgtgcgct gcgtaatgtc tgaatttctg
     5581 cacagctaaa agcacaaggt gtgccaagca tcaaaagcgc cccccagggc tttgagtgtt
     5641 ttcaggggct tatgcgtgat ttattctggt tttttcagtg ttcagtcata aaaacccctg
     5701 ccaaaccctg ctttggcatg cactgccttg gcgcaattgg cccgctgaca gcgcgcgcgc
     5761 cacgcggccc ggcggcgaca cgcgctggct gatttggtct gcgcacacgg cgtgtgcccc
     5821 acaatcgcgc ctgaagtcag tcaagcacca aaatggcgcg gctgcatgca cgcttgccga
     5881 tcaaatcgcc gcgcggcaca cttgccgtca cgcgccgcgt gggcgcgcca gcctcaaccc
     5941 gtcggctggg caagatggac gggaatcgtc gcggccagac ggccggggcg tgctctcgtg
     6001 tggagcagca agatgagtga aatgatttac ggcgacggcg ccatccgccg tccgggcctg
     6061 tatggctctt cgattgaaaa cacctacgcc ggcgtgctgt cgttcatgcg ccgcacttac
     6121 agccgcgagc tgaacggcgt ggacgtggtg gtgtctggcg tgccgctgga tttatccacc
     6181 accttccgct cgggcgcgcg gctgggcccg caggccatcc gcgccgccag cgtgcaactg
     6241 gccgagctca agccctaccc gtgggggttt gatccgtttg acgacctggc ggtgattgac
     6301 tacggcgatt gctggtttga cgcgcacaac ccgctgagca tcaagccgac gattgttgag
     6361 cacgcgcgca ccattctggc cagcggcgcc aagatgctca ccctgggcgg cgatcacttc
     6421 atcacctatc cgctgctgat tgcgcacgcc gaaaaatacg gcaagccgct ggccctgctg
     6481 cactttgacg cccactgcga cacctggccg gacgactcgc cggattcgct caaccacggc
     6541 accatgtttt acaaggccat caaggacggg ctgattgacc cggccaagtc tgtgcaggtg
     6601 ggcattcgca cctggaatga tgatttcatg ggggtgaagg tgctggatgc accgtgggtg
     6661 cacgaccacg gccccgaggc cgtcttggcg gaaatcacgc gggtgattgg cgaccacccg
     6721 gtgtatgtga cgttcgacat cgactgtctg gacccgtccg ccgcccccgg cactggcacc
     6781 cccgtgccgg gtgggctgtc cagcgcgcag gcgctgaaga ttgtgcgcaa tctgggtggg
     6841 ctgaatattg tcggtatgga cgtggtggaa gtggcgccca gctacgacca gagcgaaatc
     6901 accgcgattg ccgccgcaca cattgcctgt gacatgctgt gtctgctgcg caaccagaag
     6961 ctggcacgcc agggctaaga gcggctaaca aaactctcct ggcaacctgt gtggcgctcg
     7021 catgagcggc gcatgtcggc tcaggagggt ttttggcgca acgggctgtt tgctgatgtc
     7081 gcgcgggttt ttttcaagcg cgcaaagaaa aacgcccccg gcatcagccg agggcgtttt
     7141 tgaatttggt tgcggggaca ggactcgaac ctgtgacctt cgggttatga gcccgacgag
     7201 ctgccaactg ctccaccccg cgtcagagag gtgaactata cgcagggctg ttcaggctgt
     7261 caagggtttt tgttgcgctg caagaggacg gctcgcgcca tcacgccgtc cagctcaccc
     7321 agcccatttt ccagctcagc aaaatcagca ggccaaacac aatccgatac caggcaaaca
     7381 ccacaaaggt gtgggtggcg acaaagcgca gcaggccgcg caccgccaga aacgccgaca
     7441 gaaacgccgc gacaaagccc acgccaaaca ccggcacatc ggccatggaa aacgcctgcc
     7501 agtgcttgag cacatcgtaa gccgtggcgg cgaacatgat gggaatggcc agaaagaacg
     7561 aaaactcggt ggccaccttg cggtccaggc caaacagcat gccgccgatg atggtcgagc
     7621 ccgagcgcga cacgccagga atcagcgaaa acacctgggc aaagcccact ttcagcgcgt
     7681 cgcgcaggcg catatcgtcg atgcgggtaa tgcgcggctt ttgcgggcgc gactccaccc
     7741 acaaaatcac cagcccaccg accaccaatg ccaccgccac cgccacggcg ttgaacagat
     7801 aaaacttgat ggtcttgatg gtcagcacgc caatcactgc ggccggcaaa aaggccaggc
     7861 ccaggcccac cacaaagcgc agggcgacag ggtcgcggcg cacgccgcag gccagcgcgg
     7921 cgatgcgcgc gcggtattcc cagcacaccg ccagaatcgc ccccagctga atcaccactt
     7981 cgaacacctt gcccttgcca tcgtcaaagt tgatcaaatc gccagccaga atcaaatgcc
     8041 cggtgctgga aatcggaaaa aactcggtaa tgccttcaac gatgcccatg atcagggcct
     8101 tgcacaacaa caggatgtcc atcagtggct ttccgggcgc gcgtgctgcg gatgaatgcg
     8161 cgaaagtcaa aaaactgcgc cagcctggtg atcaggcggc gccatgcatc aggcacacgg
     8221 gccgcaaaag cggcccgtgt gcaagagatc aacggccgtt tttggccttg gcgatcagct
     8281 catccaccac ttgcagcaga cgggcgggct ctgcggccat ggtggcgtag cggccggcca
     8341 ccacaaaggt gggcgtgcct tccacttgat aatcacgggt catcttggcg gcgcgcgaca
     8401 cctggctggc catgccgaaa gatttgctgg tttgcaagaa cgcattgacg tccacgcctt
     8461 gcgccttcag gaacggggtc agcagttttt cgtcggccag gttttgcttg tcgcgcatca
     8521 tcgcgtcaaa caccggcttg tgcagcttgt ccagcaagcc catctggcgc tgggtggcga
     8581 acaggcgagc cagcggctcc atttccttgc tccacacaat ctgctcgcgg cggaagctca
     8641 catgcgccgg caacttggcc gcccagcttt ccaccgccgg ctccagccgg tagcaatgaa
     8701 tgcaggtgta cgagaaaaac tcgatcactt ccaccttgcc ggctggcgcg ccactgggtt
     8761 gcggggcgcg cagttcgaca taatcgcggc cgggctggat atccgccatg gccacggcgg
     8821 acaccagcag caacagggct acccaccatt ttttcagcat catgtttcct tcaatttctg
     8881 gattcggtca tgccccaaag acagccttcg gagcgctacg ctcacacttg ccgcaaggtg
     8941 cgccgccgca gggcagcgca acgatgcccg aagggttctg ttcgcggcgc ttagcgcggc
     9001 tcacaaaact cagcgaagcg atcctggcca ggcgcgcgac cgcagacagt aaacaagtac
     9061 ggcaaggtcg ggcaacaaca ccaggagggt tttgtgagcc gttcttagtc tgccttcacc
     9121 ggcgtcgggt tcaatccatt ggccttcaac tggccgcgca ggcgctcgac atcttccgag
     9181 cgggtcagcg ggccgatgcg cacccgatgc agcaaaccct tgtccgagct gttgaccgac
     9241 tggatattgg cctcgacccc cagcagcgcc aatttggctt tcatattgtc ggcgtccact
     9301 tctttttgat aggcgcccac ttgcaggtaa atctttttgc ccttgtcagc ggcctctttg
     9361 tctttggccg gatcggtggc gggtttttcc tgcggggctg gcggcggcgc attacccggc
     9421 agaatcttgt aaaagtcata ctgcggcggc tcggccttgg cgtgtttttc tgccggcgtc
     9481 tgcggagcat ccggtttagc atcggccttg cccggtctgg cgggcttgtc ttctgccgga
     9541 gcctgggacg gttgcggcgc agcggcaggc ggcgctgctg gtggcgggct ggccggtggc
     9601 gtatccagca aaggattggt aggcaggttg gcagccagat ccggcgcgcg gctaccaggt
     9661 gcgccaggct ccagcgtagc cgggcctggc gggggggccg gtctggctgg ctgcgccggc
     9721 agcgatacca tatccgaggc ttcttccttg gcgggtttgg cgtcgcgcgg ggcaaacggg
     9781 gtattggcgc gattcaggta gaacgccacc cccgcagcgc cacccacccc caccagcagg
     9841 ccaatcagca ccccggcgac cacgcccttg ccattgccgg atgatttttt ggctgacgtg
     9901 gacgctggcg tgcgcggcgc tggcgcgcct cggctggcgc cattgccacc acgcggtggc
     9961 cgagtgctgt ttttgtaatc acgggacatg ctgctggcgc gtctttctat cagatgagtt
    10021 catggccagc gcgcagcgct ggcccgctct tctcctgtcg gtggcggcag ggaagggccg
    10081 tggttccggc gatgcggaac acggcgcgct gcgattatat cagccgggag gggcgtgact
    10141 acaagccgcg acggtattga atagcctcgg caatatggcg ggcgttgacc gccgcgctgg
    10201 cttccaggtc ggcgatggtg cgcgccagcc gcaggatgcg atggtaggcg cggccagata
    10261 aatgaaagcg ctcgatggcc tgcgccgcca gcgcggagcc ttcggcgtcc gggcgggcga
    10321 tgtcgtccag cgtggcgccg tccagcaagg cattggccac cccctggcgc gccagctgga
    10381 tggcgaaagc cgcctccacc cgctcgcgca cccgcgcgct gggctcgccg ggcgcggcgc
    10441 gcagcaaatc ctgcgctggc aggctgggca cttccacccg catgtcgatg cggtccagca
    10501 atgggccgga tattttgccg cgatagcggc tgatttgctc tggcgtgcag cggcaacgcc
    10561 cggaagggtg gccgtgatag ccgcaggggc aggggttcat cgccgccacc agctgaaagc
    10621 gcgccgggaa atccacctga cgcgccgcac gcgaaatggt cacccggccg gtttccagcg
    10681 gctcacgcag catgtccagc gttttgcgct caaactcggg caattcatcc agaaacaaca
    10741 cgccatgcag ggccagcgac acctcgcccg gacgcggctg gccgccgccg cccaccagcg
    10801 ccgccgccga ggcggaatga tgcggcgcac gataggggcg ggtccagaat tgtgtcgggt
    10861 ccagcccgcc gctggccagc gagcgcacgc tggccacttc cagcgcttcg gtctcgctca
    10921 tcgccggcag aatgccgggc aagcgagtgg ccagcatgga tttgccggtg cccggcgggc
    10981 cggacagcag caggctgtgg cggccggcgg cggcgatttc cagcgcgcgc cgcgccgcca
    11041 gctggccttt caggtcgctc aggtcggggt agttcggcgc cagcgccggc ggcggtggct
    11101 cgggcgcctc cagccgggcc tggccattga gcatggcgca caccgcttgc aggctgtcgg
    11161 cctgcgccag cgaggcctgc ggccacagcg ccgcctcgcc cgcgctggcg cgcggcagca
    11221 ccagcgtgcg cccggcgcgg gtggcggcca tggccatcaa cagcgcgccc cgtattggcc
    11281 gcaactcgcc attcagcgcc agctcgccga caaactcatg acgggccaga tgctgggcgc
    11341 tcagttggcc cgaggcggcc agaatgccca gggcaatcgg caaatcaaag cgcccggagt
    11401 ccttgggcag gtctgcgggc gccagattga tggtgacgcg gcggttggga aactcaaacc
    11461 ccgccagctg tatcgccgcg cgcacgcgct cgcggctttc cttgacttcg gtgtcgggca
    11521 ggccgaccaa ggtcagcgag ggcaggccgt tggccagatg gacttccacg gtgacttccg
    11581 gggcgtccgt tccggccagg gcccggctgg tgatccgcgc cagcgacacg gcggggcgtc
    11641 ttaggagtgg ccctgagcgg cttgttccgc ctcggcttct accgccgcgg cggcgtcggc
    11701 tgccggctcg gccggcagca ccagggcttc cagcttggcc agacgggctt ccagctgttc
    11761 cagtttttca cgggtgcgcg ccagcacttc ttgctgcacg tcgaattctt cgcgggtgac
    11821 cagatccagc ttggtgaagg ccgagcccat caggctgcgc aggttttttt ccagatcctt
    11881 ggccgggctg gaggccaggg tgtcgccaat cttggcgctg atttcgtcga acagtttttg
    11941 gctcagca
//
LOCUS       DAOPYX010000052        11924 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_6050_length_11924_cov_8.423962, whole genome shotgun sequence.
ACCESSION   DAOPYX010000052 DAOPYX010000000
VERSION     DAOPYX010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 11924)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11924)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11924
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6050_length_11924_cov_8.423962"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(142..1671)
                     /gene="purF"
                     /locus_tag="PKN55_04255"
     CDS             complement(142..1671)
                     /gene="purF"
                     /locus_tag="PKN55_04255"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004283549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HNX70486.1"
                     /translation="MCGIIGVVGQTPVNQLLYDGLQVLQHRGQDAAGIVTANGNSFHM
                     HKGSGMVRDVFRTRNMRSLLGKAGIGHVRYPTAGSASSLAEAQPFYVNSPFGIVLAHN
                     GNLTNTEALKRSVFEKDLRHINTQSDSEVLLNVFAHELEHYIEGTQLSVEAIFKGVAG
                     VHRRAKGAYAVVALIAGHGLVAFRDPCGIRPLVLGRNDAGEYMLASESVALDCSGFTL
                     VRDVAPGEAVYITFAGELHSRQCAEQSKLAPCLFEYVYFARPDSIIDGASVHAARLNM
                     GEKLAEKIRAQGIELDVVIPIPDTSLPSALELAKCLNLPFRMGFIKNRYVGRTFIMPG
                     QAVRKKSVRQKLNPIASEFAGKNVLLVDDSIVRGTTSHEIVQMARDAGAKKVYFASAA
                     PAVRFPNVYGIDMPTRAELLATNRDDVQIAAEIGADAVIYQDLEALKQAVREASHPDA
                     LALNVFETSCFDGHYITGDIDEAFLANIEARRKESMASREESQGMVDLNLNVAEQNLV
                     Q"
     gene            complement(1943..2545)
                     /locus_tag="PKN55_04260"
     CDS             complement(1943..2545)
                     /locus_tag="PKN55_04260"
                     /inference="COORDINATES: protein motif:HMM:NF014704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HNX70487.1"
                     /translation="MNTLDWGLLAILLLSALYGLRRGLVAEVLILAGWLGSFLLARMA
                     AGTVGQLLPLGESAEGPRWLAGFVLTMLAGWLGFFLLRQVIGGMLSVAGLSGVDRVLG
                     MGFGLVRGALLLTVLTLLVGLTDLPQHTLWQHSAVARPLERLALTARAWLPADIAARV
                     HFAQADDAAPLSGWVSPQVSDMVQPAMPASPPTMANPGRR"
     gene            complement(2545..>2917)
                     /locus_tag="PKN55_04265"
     CDS             complement(2545..>2917)
                     /locus_tag="PKN55_04265"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HNX70488.1"
                     /translation="AAILSGKESKPESKKDKEASKPEPKTEKSADKPAEKKTEDKPKD
                     SGGKQRYVIQLAALSDPAKADALKQKLSGLGVSASVSRAETSKGEIHRVRVGPFASQD
                     EAKAVMNKLNAAGVSGIMVPQ"
     assembly_gap    2918..3017
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3018..3513)
                     /locus_tag="PKN55_04270"
     CDS             complement(<3018..3513)
                     /locus_tag="PKN55_04270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX70489.1"
                     /translation="MSAMSSQQDELDQLRKRARRRLVGAIVMSLIAGGVMLRVLDSKP
                     QHEMRPETVDIVSNLPGQQPAKPAGEAAATAAPAEGGSTSTPAASTPPADAKATAPAG
                     AAQTAAPAPAAPSAEAAPAAVAPPAPAPVPKLAAAMPAPVVEPSVATVKPPVAAAKAE
                     PKPEA"
     gene            complement(3688..4965)
                     /gene="folC"
                     /locus_tag="PKN55_04275"
     CDS             complement(3688..4965)
                     /gene="folC"
                     /locus_tag="PKN55_04275"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012697584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HNX70490.1"
                     /translation="MSQNLTLADWLTRLEALNINSIDLGLDRVGAVRDAMGLIPPCPV
                     ITVAGTNGKGSVCAMLSTILHAAGYRVGTFTSPHLLAYNERVAIDLKPASDAQLAAGF
                     AAVEAGRGDTRLTYFEFSTLAAMHTFISAQVDVIVLEVGLGGRLDATNAFDPSCAVLT
                     SVDLDHQDWLGGTREAIGREKAGIFRAATPAICADPRPPRAVIEHAEQIGARLLCLGQ
                     DFGFAKQEDSPQWNFWLGGARRSALPIPALRGGYQLANASAALAALETLRDALPVGMG
                     DIRRGLLEVEWPGRFQVLPGRPVTVLDVAHNPQAARALATNTLSLGFAEQRYAVFGML
                     GDKDADSVIEVMREHIDTWLLPPLDSPRALSPAALAEKLAAHGARDVRQCDSLDQAWR
                     LATSLAGENDRITVFGSFYTVAGVMQARLAARQ"
     gene            complement(4985..5401)
                     /locus_tag="PKN55_04280"
     CDS             complement(4985..5401)
                     /locus_tag="PKN55_04280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX70491.1"
                     /translation="MRPFVAPLRRSPRLLAALLAAYAAAWLAISQLPLAPALLAVALS
                     GLALWRGLRQQGWLGVPLPQLEVSARGELIAHWPSHAEQVGVRADSLALPWLIVLKLN
                     TQRGRLALLLTVDSAPADALRRLRVYLRWAQPTTVA"
     gene            complement(5410..5757)
                     /locus_tag="PKN55_04285"
     CDS             complement(5410..5757)
                     /locus_tag="PKN55_04285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012697586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX70492.1"
                     /translation="MYFVDRAVAVIKPKQAFADWLNAVPDNDMELSLDSLRADSTVIL
                     IPEFNEPEEGVAYIDDIFEQLFAMELSSWYDDPSLWPQDRSLKTFWEWFDVEIHSTLI
                     DSVDADLNNAPLE"
     gene            6492..10124
                     /gene="putA"
                     /locus_tag="PKN55_04290"
     CDS             6492..10124
                     /gene="putA"
                     /locus_tag="PKN55_04290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012697331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0004657 - proline dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trifunctional transcriptional regulator/proline
                     dehydrogenase/L-glutamate gamma-semialdehyde
                     dehydrogenase"
                     /protein_id="HNX70493.1"
                     /translation="MPSFTELSRAQSPLRDAITSAYRRDERECVHTLIPQASLSPAQV
                     LDVKGLARRLVTEVRAKRTRASGVDALMHEFSLSSQEGVALMCLAEALLRIPDKHTAD
                     RLIRDKISKGDWKAHLGQSQSLFVNAATWGLVVTGKLVSTHSETSLSSALTRLIAKQG
                     EPVIRKGVDLAMRMLGKQFVTGETIDEALANGREREARGYRFSYDMLGEAAMTEADAQ
                     RYLADYEMAIHAIGKASAGRGVYEGPGISVKLSAIHPRYSRAQRDRVMNELLPRLKKL
                     LVLAKQYNIGLNIDAEEADRMELSLDLLEALAFDPELAGWNGIGLVVQAYQKRCPYII
                     DHLIDLARRSHHRFMVRLVKGAYWDAEIKRAQVDGLPGYPVYTRKVYTDVSYLACAKK
                     LLAAQDAIYPQFATHNAYSLAAVYELGEGKDYEFQCLHGMGETLYDQVVGDDKLGRAC
                     RIYAPVGSHETLLAYLVRRLLENGANTSFVNRIVDETVSIDDLIADPVEESIRLDGQP
                     HDKIPLPLALYGAARANSRGIDLSSEHALGVLSQGLLDSDRPDWQALPLLAAGDIASA
                     EPQVVLNPANHSDKVGVVYEASLADVQSALQAAEAAAPRWAATPVAERAACLQRVADA
                     MEDNLATLMGLAIREAGKSIPNAIAEVREAIDFCRYYAERISLEFNNDSHQALGPVVC
                     ISPWNFPLAIFTGEVAAALAAGNPVLAKPAEQTALIATLAVRLFHAAGVPRDVLQLLP
                     GRGEVVGAALTGDPRVKGVIFTGSTEVAKLIHRTLAARSEEVPLIAETGGQNAMIVDS
                     SALPEQVVADVINSAFDSAGQRCSALRILCLQEDIADKVVTMLKGAMDQLTVGNPAQL
                     ATDIGPVIDAEARAGLLAHIERMKGEAKGCYQVRLGEACANGTFVPPTLFEIRSIDQL
                     KREVFGPVLHVIRFAAPELPQLIRDINATGYGLTLGVHSRIDDTIDLVCDTAHVGNIY
                     VNRNIVGAVVGVQPFGGEGLSGTGPKAGGPLYLHRLARASVAPQLTADTQSPALERLA
                     QLRDALPKLNCLNRHECEQLMHWAEQAAKLSPLARAVTLPGPTGETNSLRFAPRGTVA
                     CVADHAPSLVAQLIAALATDNRVLLGGAAADVVMHALPQTLAIAWLASPVTQPTLNAV
                     LFEGAADSEAALRAALAERDGPLVALHVCVQPTEADLYRLVAERTVSVNTTAAGGNAS
                     LMTIGD"
     gene            10477..11670
                     /locus_tag="PKN55_04295"
     CDS             10477..11670
                     /locus_tag="PKN55_04295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011135091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HNX70494.1"
                     /translation="MSHKLLTISCAVAAALSIAACGKKEEAKPADAAAPAAAPAAAGD
                     VVVKLGFAAPLTGPQAHYGEEYKNGVTLAIEDANAEKPTIDGKPVKFELLAEDDLADP
                     KTATQVAQKFVDAKVNGIIGHFNSGTAIPASKIYSDAGIPQVAMATAPAFTQQGFKTT
                     FRSMTSDTQQGTVMGKFVVETLKAKKIAIIDDRTAYGQGLADEFEKAVKANGGEVVKR
                     EFTTDKATDFAAILTTLKATNPDVVFYGGADAQSAPMAKQMKRLGLKAPLVSGEMTKT
                     PTFLKLAGKEAEGSIASLAGLPLDQMPKGKDYETRYKARFNADVATYSPYGYDATRVV
                     IEAMKKANSVEPSKYLPELAKIEYAGVTSSSWAYDDKGDLKDGGITVYKVENGEWKVM
                     QTIGK"
BASE COUNT         2186 a         3980 c         3700 g         1958 t
ORIGIN      
        1 cttgccggca tgaaaaacgc cacgccatgc cttacggggc atggcgtggc gtgggtgcca
       61 cagcactttc atccaatcgc gccaccaaca ccactcaggc agagccgtga gatggcgagc
      121 ggagggtttg gtgagcggcg cttactgcac caggttctgt tccgccacgt tcagattcaa
      181 atccaccatg ccttgcgatt cttcgcggct ggccatcgat tccttgcggc gcgcttcgat
      241 attggccaaa aacgcttcgt cgatgtcgcc agtgatgtaa tggccgtcaa agcacgaggt
      301 ttcgaatacg ttcaatgcca gcgcgtcggg gtggctggcc tcgcggacgg cttgcttgag
      361 cgcttccagg tcttgataaa tcaccgcatc cgcgccgatt tcagcggcaa tctgcacgtc
      421 gtcgcggttg gtggccagca gctcggcgcg ggtcggcata tcgatgccgt acacattggg
      481 gaagcgcacc gccggcgcgg ccgaggcaaa gtacactttc ttggcgccgg cgtcgcgcgc
      541 catctgcacg atttcgtggc tggtggtgcc gcgcacgatg gaatcgtcca ccagcagcac
      601 attcttgccg gcaaattcgc tggcgatcgg attgagcttt tggcgcaccg actttttgcg
      661 caccgcctgg cccggcatga tgaaggtgcg gccaacatag cggtttttga tgaagcccat
      721 gcggaacggc aggttcaggc acttggccag ctccagcgcc gacggcaggc tggtgtcggg
      781 gatggggatc accacgtcca gttcaatgcc ctgcgcgcgg attttctcgg ccagcttctc
      841 gcccatattc agtcgggcgg cgtgcaccga ggcgccgtcg atgatcgaat ccgggcgggc
      901 aaaatacaca tattcaaaca agcacggcgc cagcttgctc tgttcggcgc attggcggct
      961 gtgcaactcg ccggcgaagg tgatgtacac cgcttcgccc ggcgccacat cgcgcaccag
     1021 ggtaaagccg gaacagtcca gcgccaccga ttccgaggcc agcatatatt cgccagcgtc
     1081 gttgcggccc agcaccagcg ggcggatgcc gcacggatca cggaaggcca ccaggccatg
     1141 gccggcaatc agcgccacca cggcgtaagc gcccttggcg cggcgatgca cgccggccac
     1201 gcccttgaaa atcgcctcga ccgacagctg ggtgccttcg atatagtgtt ccagctcgtg
     1261 ggcaaacaca ttcagcagca cttccgaatc ggactgggtg ttgatgtggc gcaggtcttt
     1321 ttcgaacacc gagcgcttga gcgcctcggt gttggtcaga ttgccgttgt gcgccagcac
     1381 gatgccaaac ggcgaattga cataaaacgg ctgcgcctcg gccaggctgg aggccgaacc
     1441 ggccgtcggg tagcgcacat ggccaatgcc ggccttgccc agcagcgagc gcatgttgcg
     1501 ggtgcggaac acgtcgcgca ccatgccgct gcccttgtgc atgtgaaagc tgttgccgtt
     1561 ggcagtgaca atgccggcgg cgtcctggcc gcgatgctgc aacacttgca ggccgtcata
     1621 cagcagctga ttgaccggcg tttgtcccac taccccaata attccgcaca taacagagaa
     1681 ttcccaacat gctggcggac gctgtccgcc ggatgagacg gccgcgtggc cgccacgaaa
     1741 aaacccagca aaagatcaca ccggccaaga ccggcgccat atcgcgggcc acgcccgcga
     1801 cgaaaaatcc acttagagtc gctcacaaaa ctcagcgaag cggccctggc taggcgtgtg
     1861 accgcagaca gtacaacaag tacgacaagg tcaggtcaca caacaacgcc agggggtttt
     1921 gtgagcggct cttaccccgc catcagcgac gccccggatt ggccatggta ggcgggctgg
     1981 ccggcatggc cggctgcacc atatcgctga cctggggtga tacccagcca ctcagcggcg
     2041 ccgcatcgtc agcctgggca aaatgcacgc gggcggcgat gtctgccggc agccaggcgc
     2101 gcgcggtcag cgccaagcgc tccagcggcc gcgccaccgc cgagtgttgc cacagcgtgt
     2161 gttgaggcag atcggtcaag cccaccagca gggtgagcac tgtcagcagc agcgccccgc
     2221 gcaccaggcc aaaacccatg cccagcaccc gatccacccc ggacaacccg gccacgctca
     2281 gcatgccgcc aatcacctgg cgcaacagga aaaaacccag ccagccggcc agcatggtca
     2341 gcacaaaccc ggccagccag cgcgggcctt cggcagactc tcccagcggc aacaactggc
     2401 ctaccgtgcc ggccgccatc cgcgccagca aaaaactgcc cagccaaccg gccagaatca
     2461 atacctcggc caccagcccg cgccgcaggc catacagcgc tgacagcaac aagatggcca
     2521 gcagccccca atccagcgta ttcattattg cggcaccatg atgccggaca ccccagccgc
     2581 gttcagcttg ttcatcaccg ccttggcttc gtcctgcgag gcaaacggcc ctacccgcac
     2641 acggtggatt tcgcccttgc tggtttccgc gcggctgacc gaggcggaaa cgcccaagcc
     2701 cgagagcttt tgcttgagcg catcggcttt ggccgggtca gacagcgcgg ccagctggat
     2761 cacatagcgt tgcttgcccc cgctgtcctt gggcttgtcc tcggtttttt tctcggcggg
     2821 tttgtctgcc gatttctcgg ttttcggctc gggcttgctg gcttccttgt cttttttcga
     2881 ctcgggtttg ctttccttgc cgctgagaat ggcagccnnn nnnnnnnnnn nnnnnnnnnn
     2941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3001 nnnnnnnnnn nnnnnnntgg cttcaggttt cggttcggcc ttggccgctg cgaccggcgg
     3061 tttaaccgtg gcgacactgg gttccaccac gggcgccggc atggcggcgg ccagcttggg
     3121 caccggcgcg ggagccggcg gcgcgaccgc agccggggcg gcttctgccg acggcgctgc
     3181 cggtgcagga gctgccgtct gggcggcgcc agccggggcg gtggccttgg cgtctgctgg
     3241 cggcgtgctg gccgcgggcg tcgatgtgct gccgccctcg gccggagccg cggtggccgc
     3301 agcctcgccg gcaggcttgg cgggctgctg gcccggcaga ttgctgacaa tgtccacggt
     3361 ttccgggcgc atttcgtgtt gcggtttgct gtccagcacg cgcagcatca cgccgccggc
     3421 aatcagactc atgacgatgg cgccaaccag acgccgccgc gcgcgtttgc gcagctgatc
     3481 cagttcgtcc tgctggctgc tcattgctga catcgatcat cctgtacgag aaaacggcgc
     3541 gccgggctag gctgcgccgc aaaagtccat cattcagcgc cgcccaccaa accctccagg
     3601 cgttgttgcc acctgtcctg cctgcggccg cgcgcctggc gcaggcacgc tacgctgagt
     3661 ttggtgagtg gttctatatg ccgtggctca ctgacgggcg gcaagacggg cctgcatcac
     3721 cccggccacg gtgtaaaacg agccaaagac ggtaattcta tcattttcgc cggccagcga
     3781 cgtggccagg cgccaggctt gatcaaggct gtcgcactga cgcacatcgc gcgcgccgtg
     3841 cgcggccagt ttttccgcca gcgccgccgg gctgagcgcg cgcgggctgt ccagcggcgg
     3901 cagcagccag gtatcgatat gctcgcgcat cacctcgatc acgctgtcgg cgtctttgtc
     3961 gcccagcatg ccaaacaccg cgtagcgttg ttcggcaaag cccaggctca gggtgttggt
     4021 ggccagcgcg cgcgccgcct gcgggttgtg cgccacgtcc agcaccgtca ccggccggcc
     4081 gggcagcacc tgaaaacgcc ctggccattc tacttccaac aggccgcgcc ggatgtcgcc
     4141 catgcccacc ggcagcgcgt cgcgcagcgt ttccagcgct gccaacgccg cgctggcgtt
     4201 ggccagttga tagccgccgc gcaaggccgg aatcggcaag gcgctgcgtc gggccccgcc
     4261 cagccagaaa ttccactgcg ggctgtcttc ctgtttggca aagccaaaat cctggcccag
     4321 acacagcaag cgcgcgccaa tctgctcggc gtgttcaatc accgcgcgcg gcgggcgcgg
     4381 gtcggcgcag atggccggcg tcgccgcccg aaaaatcccg gctttttcgc ggccaatcgc
     4441 ctcgcgcgtg ccgcccagcc aatcctggtg gtccaggtcc acgctggtca gcaccgcgca
     4501 gctggggtca aaagcgttgg tggcgtccag ccggccgccc aggccgactt ccagcacaat
     4561 cacgtccacc tgggccgaga taaaggtatg catcgccgcc agggtggaaa actcaaaata
     4621 agtcagccgg gtgtcgccgc gcccggcctc gacagcggca aagccggcgg ccagttgggc
     4681 gtcgctggcg ggcttgaggt caatcgccac ccgttcgttg taggccagca gatgcggcga
     4741 ggtaaacgtc cccacccgat agccggcggc gtgcaggatg gtggacagca tggcgcacac
     4801 cgagcccttg ccattggtgc cggccacggt gatcaccggg cagggcggaa tcagccccat
     4861 ggcgtcgcgc acggcgccca cgcggtccag ccccaggtcg atgctgttga tgttgagggc
     4921 ttccagccgg gtcagccaat cggccagcgt caagttttga ctcatgatgt caggcagtgt
     4981 cctgtcaggc gacggtcgtc ggctgcgccc agcgcagata cacccgcagc cggcgcaagg
     5041 cgtcggcggg ggcactgtcc acggtgagca gcaaggccag gcggccgcgc tgggtgttca
     5101 gtttcagcac aatcagccaa ggcagcgcca ggctgtcggc gcgcacgccc acctgttcgg
     5161 cgtggctggg ccaatgggca atcagctcgc cgcgcgcgct cacttccagc tgcggcagcg
     5221 gcacgcccag ccagccttgc tgacgcaggc cgcgccacaa ggccagcccg ctcagcgcca
     5281 ccgccagcaa ggctggcgcc agcggcaact ggctgatggc cagccaggcg gcggcgtagg
     5341 cggccaataa ggcggcaagc aaacgcggcg agcggcgcag cggcgccaca aatggcctca
     5401 tgccagcgtt cattccagcg gggcgttgtt caggtcggcg tccaccgagt caatcagggt
     5461 ggagtggatt tccacgtcga accattccca gaaggttttc agcgaacggt cttgcggcca
     5521 caggctgggg tcgtcgtacc agctggacag ctccatggcg aacagctgct cgaagatgtc
     5581 atcaatatag gccacgcctt cttccggctc gttgaattcc ggaatcaaaa tcaccgtgct
     5641 gtcggcgcgc aggctgtcca gcgacagctc catatcgttg tccggcacgg cgttgagcca
     5701 gtcggcaaag gcttgtttgg gtttgatcac cgcgacggcg cgatcgacaa aatacatggc
     5761 agacgctcca caaagtttaa gttcaggagg cgcttctcgc gcgcgaaagc ggcgtattgt
     5821 agcagggccg gcgcatgacg agtgaccgag caagggcatg cgcagctgcg cgatggccga
     5881 ttgtcgatca ggcgcaagct gaagcagccc gcaaagctct cctggctttg gcgcccggcc
     5941 cggcacacac caaaaaattt tgtctataac tgtccgctgc gtgccctgga cgccggcaag
     6001 ctccaccctg cccctggcag cggtctctgc cgtgacatcg ccgccccact catccgggct
     6061 tgacaaaacg ccctcggact tgcttcgcgc gcgacgccgc caagcaagcg tttgcctggc
     6121 gttctggcaa aaacaacact ttgttttata ttgaaattct tcccgcccac cgctccattc
     6181 gcacctatca cccccctgtt ttctgtggtt tttttgccct tggggccgtc agcggcgggg
     6241 tttttcgtgg ttttgtacgg aattgcgaca tgggcaacca tggctgtgtc atccgggggg
     6301 aaagtggatt ttccgggcgc aactttcacg acacaatgcg ccggtcactg tctgggcgac
     6361 cccgcccgca ctgtggcgag cccagccaga agcgggcctt tgccgccgcg ccggaacagc
     6421 catcctggcc agcccggcag gcaggcgatg ctgctggccg ctcgcgccga tcacctacgg
     6481 agttcatcac catgccgtcg tttaccgaac tgtcccgcgc gcaatcgccg ctgcgtgacg
     6541 ccatcacttc cgcttaccgc cgcgacgaac gcgaatgcgt tcacacgctg attccgcaag
     6601 cctcgctctc gcccgcccag gtgctggacg tcaagggcct ggcgcgccgg ctggtgaccg
     6661 aagtgcgcgc caagcggacg cgggccagcg gggtggatgc gctgatgcat gaattttcgc
     6721 tgtccagcca ggaaggcgtg gcgctgatgt gcctggccga agccctgctg cgcatccccg
     6781 acaagcacac cgccgaccgc ctgattcgcg acaagatcag caagggcgac tggaaagccc
     6841 acctggggca aagccagtcg ctgtttgtca acgccgccac ctggggcctg gtggtcaccg
     6901 gcaagctggt gtccacccac agcgaaacca gcctgtcctc ggcgctcacc cgcctgatcg
     6961 ccaagcaagg cgagccggtg atccgcaagg gcgtggacct ggccatgcgc atgctgggca
     7021 agcagtttgt cactggcgaa accatcgacg aggcgctggc caacggccgc gagcgcgaag
     7081 cgcgcggtta tcgcttcagc tatgacatgc tgggcgaagc cgccatgacc gaagccgacg
     7141 cccagcgcta tctggccgac tacgaaatgg ccatccacgc catcggcaag gcctcggccg
     7201 gccgtggcgt gtacgaaggg ccggggattt cggtcaagct gtcggccatt cacccgcgct
     7261 acagccgcgc ccagcgcgac cgggtgatga acgagctgct gccgcgcctg aaaaaactgc
     7321 tggtgctggc caagcaatac aatatcggcc tgaacatcga cgccgaagaa gccgaccgca
     7381 tggagctgtc gctggacctg ctggaagcgc tggcgtttga ccccgagttg gccggctgga
     7441 acggcatcgg cctggtggtg caggcttacc aaaagcgctg cccctacatc atcgaccacc
     7501 tgattgacct ggcccgccgc agccaccatc gctttatggt gcgcctggtc aagggcgcct
     7561 actgggatgc cgaaatcaag cgcgcccagg tggacggcct gccgggctac ccggtgtaca
     7621 cccgcaaggt gtacaccgat gtgtcttacc tggcctgcgc caaaaagctg ctggccgcgc
     7681 aagacgccat ctatccgcag ttcgccaccc acaacgccta ttcgctggcg gcggtgtacg
     7741 aactgggcga aggcaaggat tatgaattcc agtgcctgca cggcatgggc gaaaccctgt
     7801 acgaccaagt ggtgggcgac gacaaactgg gccgcgcctg ccgcatttat gcgccggtcg
     7861 gctcgcacga aactctgctg gcctacctgg tgcgccgcct gctggaaaac ggcgccaaca
     7921 cctcgtttgt gaaccgcatt gttgacgaaa ccgtcagcat cgacgacctg attgccgacc
     7981 cggtggaaga atccatccgc ctggatggcc agccgcacga caagattccg ctgccgctgg
     8041 cgctgtatgg cgcggcgcgc gccaactcgc gcggcattga tctctccagc gaacacgcgc
     8101 tgggcgtgct gtcgcaaggc ctgctggaca gcgaccgccc ggactggcaa gcgctgccgc
     8161 tgctggctgc cggcgacatc gccagcgccg agccgcaggt ggtgctcaac ccggccaacc
     8221 acagcgacaa agtgggcgtg gtctacgaag ccagcctggc cgatgtgcaa tccgcgctgc
     8281 aagccgccga agccgccgcg ccgcgctggg ccgccacccc ggtggccgaa cgcgccgcct
     8341 gcttgcagcg cgtggccgac gccatggaag acaatctggc cacgctgatg ggcctggcga
     8401 ttcgcgaagc gggcaaatcc atccccaacg ccatcgccga agtgcgcgag gcgattgact
     8461 tctgccgcta ctacgccgaa cgcatcagcc tggaattcaa caacgacagc catcaggcgc
     8521 tgggcccggt ggtgtgcatc agcccgtgga acttcccgct ggccatcttt accggcgaag
     8581 tggccgccgc gctggccgcc ggcaacccgg tgctggccaa gccggccgag caaaccgcgc
     8641 tgatcgccac cctggccgtg cgcctgttcc acgccgctgg cgtgccgcgc gacgtgctgc
     8701 aactgttgcc gggccgtggc gaagtggtgg gcgcggcgct gaccggcgac ccgcgcgtga
     8761 agggggtgat tttcaccggc tcgaccgaag tggccaagct gattcaccgc accctggctg
     8821 cgcgcagcga agaagtgccg ctgattgccg aaaccggcgg ccagaacgcc atgatcgtcg
     8881 atagctcggc gctgccggaa caagtggtcg ccgatgtgat caactcggcc ttcgacagcg
     8941 ccggccagcg ctgctcggcg ctgcgcatcc tgtgcctgca agaagacatc gccgacaagg
     9001 tggtgaccat gctcaagggc gccatggacc aactgaccgt gggcaacccg gcccagctgg
     9061 ccaccgatat tggcccggtg atcgacgccg aagcccgcgc cggcctgctg gcgcatatcg
     9121 agcgcatgaa gggcgaggcc aagggctgct atcaagtgcg cctgggcgag gcctgcgcca
     9181 atggcacgtt tgtgccgccg acgctgtttg aaatccgcag cattgaccag ctcaagcgcg
     9241 aagtgtttgg cccggtgctg catgtcatcc gctttgccgc ccccgagctg ccgcagctga
     9301 tccgcgacat caacgccacc ggctacggcc tgaccctggg cgtgcacagc cgcatcgacg
     9361 acaccattga tctggtgtgc gacaccgcgc atgtgggcaa tatctacgtc aaccgcaata
     9421 tcgttggcgc cgtggtcggc gtgcagccgt ttggcggcga aggtttgtcc ggcaccggcc
     9481 cgaaagccgg cggcccgctg tatctgcacc gcctggcccg cgccagcgtc gccccgcagc
     9541 tgactgccga cacccaaagc ccggcgctgg agcgcctggc ccaactgcgc gatgcactgc
     9601 ccaagctgaa ctgcctgaat cgccacgaat gcgagcagct gatgcactgg gccgagcaag
     9661 ccgccaagct cagcccgctg gcgcgcgccg tcaccctgcc aggcccgacc ggcgaaacca
     9721 acagcctgcg ctttgccccg cgcggcacgg tggcctgcgt ggccgaccat gcgccgtcgc
     9781 tggtggccca gctgattgcc gcgctggcca ccgacaaccg cgtgctgctg ggcggcgccg
     9841 ccgccgatgt ggtgatgcac gccttgccgc aaaccctggc catcgcctgg ctggcgtcgc
     9901 cggtgacgca gccgacgctg aacgccgtgc tgtttgaagg cgccgccgac agcgaagccg
     9961 cgctgcgcgc cgcgctggcc gagcgcgatg gcccgctggt ggcgctgcat gtctgcgtgc
    10021 agccaaccga ggccgacctg tatcggctgg tggccgaacg cacggtcagc gtcaacacca
    10081 cggcggccgg cggcaacgcc agcctgatga ccatcggtga ttgagccact gggcgccggc
    10141 cgctgcaatg cggccggcgc ccgatgattt tatgccccct cctggtgcat ccgcatctgg
    10201 agggggtggc gcatcaagat ctatataagg tagtgcgcca aaatggggcg atgcagatga
    10261 tgggtctgcc cgcccgcttc agggagaacc ccgcttgccc tcagtctgcg cagccaaaca
    10321 ggcagacgct gcggcaaacg gggagatatt gcgcgtgaaa cccgattcgg ccggtttcac
    10381 gtacaatccg gggcgcgcgc cacaagcgcg cccagttaca ggtgttgccg gccccaccgg
    10441 cggccggttt cattccacaa tcagaggaag aagactatgt ctcacaaact tttgaccatc
    10501 tcgtgcgcag ttgccgccgc cctgtccatc gccgcttgcg gcaaaaagga agaagccaag
    10561 ccggctgacg ccgccgcccc ggctgcggcc ccggctgctg ctggcgatgt ggtggtcaag
    10621 ctcggctttg ccgccccgct gactggcccg caagcgcact atggcgaaga gtacaaaaat
    10681 ggcgtgaccc tggccatcga agacgccaac gccgaaaagc cgaccatcga cggcaagccg
    10741 gtgaagttcg agctgctggc cgaagacgac ctggccgacc cgaaaaccgc cacccaagtg
    10801 gcgcaaaagt ttgttgacgc caaagtgaac ggcatcatcg gccacttcaa ctctggcacc
    10861 gccatcccgg cttccaagat ctactctgac gccggcatcc cgcaagtggc catggccacc
    10921 gccccggcct tcacccagca aggcttcaag accaccttcc gctcgatgac ctccgacacc
    10981 caacagggca ccgtgatggg caagttcgtg gtggaaaccc tgaaggccaa gaaaatcgcc
    11041 atcatcgacg accgcaccgc ctacggccaa ggtctggcgg atgagttcga aaaggccgtc
    11101 aaggccaacg gcggcgaagt ggtcaagcgt gaattcacca ccgacaaggc caccgacttt
    11161 gccgccatcc tgaccaccct gaaggccacc aacccggatg tggtgttcta cggcggcgcc
    11221 gacgcccaat ccgccccgat ggccaagcag atgaagcgcc tgggcctgaa ggccccgctg
    11281 gtgtcgggcg aaatgaccaa gaccccgacc ttcctgaagc tggccggcaa agaagccgaa
    11341 ggcagcatcg cctcgctggc tggcctgccg ctggaccaga tgcccaaggg caaggactac
    11401 gaaacccgct acaaggcccg cttcaatgct gacgtagcca cctactcgcc gtacggctac
    11461 gacgccaccc gcgtggtgat cgaagccatg aaaaaagcca actcggttga gccgagcaag
    11521 tacctgccgg aactggccaa gattgaatac gctggcgtga cctcgtccag ctgggcctac
    11581 gacgacaagg gcgacctgaa agacggcggc atcaccgtct acaaggtgga aaacggcgaa
    11641 tggaaggtga tgcagaccat cggcaagtaa tcgccatggg ctgatggcct gttgctgcac
    11701 ccgacacccc gctgcgccat gcgtgagcgg ggtgtttagc gtgaaacact cagatttttg
    11761 tgcgcaaaaa agcatcgcga acaatcaaga cgcgatgcga gcagcaaaga gcaggggcgg
    11821 tgaactcatt taccttggcc aagcactatc cctagtgcct ctaaccgtgg cacgtggtga
    11881 agctgccaga agccaagcgt ggctttgtgc tgctgccgcg tcgc
//
LOCUS       DAOPYX010000053        11840 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_6127_length_11840_cov_10.106916, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000053 DAOPYX010000000
VERSION     DAOPYX010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 11840)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11840)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11840
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6127_length_11840_cov_10.106916"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..924
                     /locus_tag="PKN55_04300"
     CDS             <1..924
                     /locus_tag="PKN55_04300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008954704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HNX70495.1"
                     /translation="AEADESDASFLCLQPVMAVVTNIDADHMDTYDHSLEKLKQAFVD
                     FLHRLPFYGHAVLCVDDANVRSILPQVKRPVTTYGFAADAQVRAENVVPDGQCMRFDA
                     VWVNGETHREPIVLNTPGRHNVLNALATIAIALECGAGIDAIRQGLAQFSGVGRRFQR
                     YGEVPVPTGGSFTLVDDYGHHPVEMAATLAAARGAFPGRRLVLAFQPHRYSRTRDLFE
                     DFVRVLGTVDGVLLTEVYPAGEAPIVAADGRALARALRVAGKLEPVFVEQVAELPAAI
                     MEFVRDGDVVLTMGAGSIGATPGKLVAAARG"
     gene            935..1846
                     /locus_tag="PKN55_04305"
     CDS             935..1846
                     /locus_tag="PKN55_04305"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008954705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HNX70496.1"
                     /translation="MKQYGKVAVMFGGASAEREVSLMSGQAVLDALRRQGVDAHAFDP
                     AEKPLSALQDEGFDRVFIALHGRGGEDGTVQGALELMKLPYTGSGVMASSIGMDKWRT
                     KLVWQAVGIPTPEYEVLTADSDFAEVEARLGLPLMVKPAHEGSSIGIVKVSEPGQLAA
                     AWHDAASLDRLVIAERFIAGREYTAPVLDDQALPLIRIAAPEGNYDYQHKYFTDDTQY
                     FCPCGLPENLEAQMRDTVLRAFKVLGAEGWGRIDIMLDDAGRFYLLEINTSPGMTSHS
                     LVPMAARVAGIEFDALCLRILDGAHVG"
     gene            1836..>2071
                     /locus_tag="PKN55_04310"
     CDS             1836..>2071
                     /locus_tag="PKN55_04310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HNX70497.1"
                     /translation="MWDNHRLLRGVANLLLLASILTLLVAGAQWVVRSGLLPIRAVHI
                     RSKLTHVTQEQLRYVAEHELKGSFLTVDITETRAA"
     assembly_gap    2072..2171
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2172..2429
                     /locus_tag="PKN55_04315"
     CDS             <2172..2429
                     /locus_tag="PKN55_04315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012698493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HNX70498.1"
                     /translation="VLPEELKLSARRAWQVKLDNSVILELGRQDIGLRVGRFAASWRD
                     QLSRLSYKIEYVDLRYPNGFAVRMPDYKPGKGAPAMPRPAA"
     gene            2459..3697
                     /gene="ftsA"
                     /locus_tag="PKN55_04320"
     CDS             2459..3697
                     /gene="ftsA"
                     /locus_tag="PKN55_04320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008954707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HNX70499.1"
                     /translation="MSKPREPKNLLVGLDVGTSKIVAAVAEVLEDGALNIIGMGQTSS
                     RGLKKGMVVNIESTVQAIQRAVEEAELMADCTIHQVFTGIAGSHIKSVNSHGMVAIRE
                     REVTQADIDRVIETARAVTTPPDHQVLHILTQEFSIDGQEDVREPLGMSGVRLETRVH
                     IVTGAVSAAQNILKCVRRCNLDVADLILQPLASATAVLTDDERDLGVCLIDIGGGTTD
                     IAVYTRGAIRHTAVIPIAGDQITNDIAMALRTPTKEAEDIKLVHGCALMQLADPADMI
                     DVPGVGERGPRQLSRATLAEVIEPRVEELFQLIQQELRRTGFEDVLSSGIVLTGGAAK
                     MPGMVELAEEVFHMPVRVGVPKYFGGLAEAVKDPRFSTSIGLLLIGKDQLQKTAGPKP
                     ETASVGGLFSRMKAWFAGNF"
     gene            3776..4975
                     /gene="ftsZ"
                     /locus_tag="PKN55_04325"
     CDS             3776..4975
                     /gene="ftsZ"
                     /locus_tag="PKN55_04325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011137884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HNX70500.1"
                     /translation="MSSNMVFEMVQEIAPEAVIKVIGVGGGGCNAVDNMIESQVKGVE
                     FIAANTDAQALRRNRAPVQIQIGQTLTKGLGAGANPDVGRSAAIEDRDRIAEVLRGAN
                     MVFIATGMGGGTGTGAAPVIAEIAKEMGILTVGVVTRPFSHEGRRHQVAGAGIEELKK
                     YVDSLIVIPNEKLLQVLGSDVTMRQAFRAADDVLRGAVAGIAEVITCPGFINVDFADV
                     KNVMGQMGMAMMGSAVASGLDRSRIAAEEAVASPLLDDISLDGARGVLVNISTAPGCL
                     LLREYHEIMEIIRNYAHADAEVKFGTAEVDGMSEDEIRVTLIATGLGGGKKPAREERR
                     EQLKVVKNGTDGTTMEVPDFQQPDFDKPSVFRARSGRGAPQQVDFSNPSVGDSFDIPA
                     FLRRQAD"
     gene            5235..6146
                     /gene="lpxC"
                     /locus_tag="PKN55_04330"
     CDS             5235..6146
                     /gene="lpxC"
                     /locus_tag="PKN55_04330"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004284485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HNX70501.1"
                     /translation="MHQRTLSQTISASGVGLHSGERVKLTLRPAPPDTGIIFRRTDLT
                     PPVELRAEPALVNDTRLSSTLVDPNGVRVATVEHLMSALAGLGIDNLIVEMTGPETPI
                     MDGSAAPFIYLLDSAGISEQTALKRYIRVLKPLEVVEGDKWVKLTPYDGFTVSLEIEF
                     NHPVFRRTPQHLTIDFAAQSYIDEVSRARTFGFMHEVEYMRMNGLGLGGNLDNAIVID
                     DDSVLNKDGLRFNNEFVRHKILDAIGDLYMIGHPLIAAFSGFKSGHAMNNKLLRELQA
                     RPDAWEYATFRRPSDAPSSFHRLPQAA"
     gene            6212..6697
                     /gene="moaC"
                     /locus_tag="PKN55_04335"
     CDS             6212..6697
                     /gene="moaC"
                     /locus_tag="PKN55_04335"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HNX70502.1"
                     /translation="MSALTHFNADGQAHMVDVGDKADTHRRAIASGVIRMQPDTFALV
                     QSGSHKKGDVLGIARVAAIMASKHTSTLIPLCHPLALSHVDVEFQLDAAASAITCRVT
                     AETVGKTGVEMEALTAVQIGLLTIYDMCKAVDRGMVIGEVRLLEKQGGKSGLWRAEGL
                     D"
     gene            complement(6783..7901)
                     /gene="proB"
                     /locus_tag="PKN55_04340"
     CDS             complement(6783..7901)
                     /gene="proB"
                     /locus_tag="PKN55_04340"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014085745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HNX70503.1"
                     /translation="MQSVIHASQRIVVKVGSSLVTNDGKGLDHAALSRWASEIADLTR
                     RGKQVVLVSSGAIAEGLQRLGWATRPKAVHELQAAAAVGQMGLVQAYETAFRAEGLKT
                     AQVLLTHEDLADRTRYLNARSTLITLLKLNVVPIINENDTVATSEIRFGDNDTLGALV
                     TNLIEADALIILTDQQGLYTADPRKHADAEFVHEAQAGDPRLEDMAGGAGSSVGTGGM
                     ITKIIAAKRAARSGAATVIACGREANVLSRLADGEAIGTQLIAADTPLLARKQWLADH
                     LQLAGRLVLDEGAAHAVVHKGTSLLPIGVVAVEGEFLRGEALACVDPQGHEVARGLVN
                     YSSDETRLIMRHATREIESILGYMGEPELIHRDNMVSV"
     gene            8077..8550
                     /locus_tag="PKN55_04345"
     CDS             8077..8550
                     /locus_tag="PKN55_04345"
                     /inference="COORDINATES: protein motif:HMM:NF022479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2721 domain-containing protein"
                     /protein_id="HNX70504.1"
                     /translation="MELTTAIPTLAHAIQQSVAPVFLLTGVGSLLGVLVNRLGRIVDR
                     YRQLRSLPAEQITPHRAEMALLQRRSRLIHFAIRYSTLCALLICVVISLLFIGVELGS
                     ELSPLIAALFVCGMLSLIASLVCFLKEISLATWRLEAAFDADLRQDTPPPPDGQS"
     gene            complement(8611..9963)
                     /gene="bioA"
                     /locus_tag="PKN55_04350"
     CDS             complement(8611..9963)
                     /gene="bioA"
                     /locus_tag="PKN55_04350"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011137756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HNX70505.1"
                     /translation="MPSTDWLSRSTRAVWHPCTQMQRHETFPLIPIARGEGVWLIDFD
                     GKRYLDAVSSWWVNLFGHANPRINAALIDQLGRLEHVMLAGFTHQPVVELSERLAALS
                     GLGHAFYGSDGASATEIALKMSFQYWQQSGAPQKSRFIHLEHSYHGETLGALAVTDVP
                     LFRQTYAPLLNAALTAPSPDARLAGPGETAETVARRAAAGLEALLQQHAGEVAALIIE
                     PLVQGAAGMAMHHPLYLAEARRLCSQYGAHLIADEIAVGFGRTGTLFACEQAGVRPDF
                     LCLSKGITGGYLPLSCVLTTDEVYAAFYHPDIRRGFLHSHSYTGNALACRAALAVLDI
                     FAEDDVLAQNRAKAAAFAPLLAPLQAHPAVRHFRQQGMIWAFDVATTRSDFALAFFSA
                     MLEQGCLLRPIGQTVYFMPPYVIEAEQMAQLVNATLRTLDALVGDGNGQAGEQEPAVP
                     "
     gene            10059..10571
                     /locus_tag="PKN55_04355"
     CDS             10059..10571
                     /locus_tag="PKN55_04355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HNX70506.1"
                     /translation="MKQVFRARRATPAQQGLTLIGQMMVLLVAGALGAVALSSWLDVQ
                     VRERATEAYNQLEAIKPAAEKLGREQRGQPWPASLAMATLGEAPAGSRFVAGSWQASA
                     QDRLLTVSVQLQDTGRHLDGQRLLLKGWLEEDGDVRWVCASDVDPRWHKQLPAPCQYK
                     PSRSPEPKSE"
     gene            10840..11727
                     /locus_tag="PKN55_04360"
     CDS             10840..11727
                     /locus_tag="PKN55_04360"
                     /inference="COORDINATES: protein motif:HMM:NF024676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HNX70507.1"
                     /translation="MSVDPPKHEMLANRLAEILLKLNLDERPSRQELAAEFKVTERTI
                     YRDLNRLGSIVEQLPDGRYQLASEYRGKLKPKDLQSFAQLAGVEQLFPDASPQFLVAL
                     LDTLCQGSLLVHGHQYERFKPHDKSFDLLNEAVTGQRICRLTYRDKPRELHPYRLINK
                     NGIWYLAATEQGQLKAFAFSRISQLSVTEQTFTPDATIQANIENEDDIWFSREKTEVL
                     LSIAPEIAYYFRRRKLLPKQELVRELESGGLIVSSQISHVDQILPLAQFWLPRIRVVE
                     PAWLRERLETVLRDYLEQK"
BASE COUNT         2157 a         3557 c         3804 g         2222 t
ORIGIN      
        1 gccgaagcgg atgagtcgga tgcttcgttt ttgtgtctgc aaccggtgat ggccgtggtg
       61 accaacatcg acgccgacca tatggatact tacgaccaca gcctggaaaa acttaagcag
      121 gcttttgtcg attttttgca tcggctgcca ttttatggcc atgccgtgct gtgcgtggac
      181 gacgccaatg tgcgcagcat cctgccgcag gtgaagcgcc cggtgaccac ttacggcttt
      241 gcggccgacg cccaggtgcg cgccgagaat gtcgtgccgg atggtcagtg catgcgcttt
      301 gacgcggtgt gggtgaatgg cgagacgcat cgtgagccca tcgtgctgaa cacgccgggc
      361 cgccacaatg tgctcaacgc gctggccacc atcgccatcg ccctggagtg cggcgccggc
      421 atcgacgcca ttcgtcaggg tctggcgcag ttttctggtg tgggccgacg ctttcagcgc
      481 tatggcgaag tgcctgtgcc gacaggtggc agcttcacgc tggtggatga ctacggccat
      541 catccggtgg aaatggccgc cacgctggcc gccgcgcgcg gggcatttcc ggggcgacgc
      601 ctggtgctgg ccttccagcc gcatcgctac tcgcgcactc gtgacctgtt cgaggacttt
      661 gtccgcgtgc tgggtacggt ggatggtgtg ttgttgactg aagtctaccc ggcgggcgaa
      721 gcgccgattg tggcggccga tggccgcgca ctggcgcgcg ccttgcgcgt ggctggcaag
      781 cttgagccgg tgtttgtcga gcaggtggcc gaattgccgg cggccatcat ggaatttgtc
      841 cgcgacggtg atgtggtgct gaccatgggc gcgggctcga ttggcgccac gccgggcaag
      901 ctggtcgccg ctgcgcgtgg atgaggaagc aggaatgaag caatacggca aagtggcggt
      961 gatgtttggc ggcgcctccg ccgagcggga agtctcgctg atgagcggtc aggctgtgct
     1021 tgacgccctg cgtcgccagg gcgtggacgc ccacgccttc gacccggcgg aaaaaccgct
     1081 gtcggcactg caggatgaag ggtttgatcg cgtgttcatc gccctgcacg gtcgcggtgg
     1141 cgaagatggc acggtgcaag gcgcgctgga actgatgaag ctgccctaca ccggcagtgg
     1201 cgtgatggcg tccagcatcg gcatggacaa atggcgcacc aagctggtgt ggcaggcggt
     1261 gggcattccc accccggaat acgaagtgct gaccgccgac agtgattttg ccgaggtgga
     1321 agcgcggctg ggcttgccgc tgatggtcaa gccggcgcac gaaggctcgt cgatcggcat
     1381 cgtcaaggtc agcgagccgg ggcaactggc tgccgcctgg cacgacgccg ccagcctgga
     1441 ccgcctggtg attgccgagc gtttcatcgc cgggcgtgaa tacaccgcgc cggtgctgga
     1501 cgatcaggct ttgccgctga ttcgcattgc cgcaccagag ggcaattacg actatcagca
     1561 caagtatttt accgacgaca cccagtattt ctgtccgtgc ggtttgccgg agaacctgga
     1621 agcgcagatg cgcgatacgg tgttgcgtgc gttcaaggtg ctgggggccg aaggctgggg
     1681 tcgcatcgac atcatgctgg atgacgccgg ccggttttac ttgctggaaa tcaacacctc
     1741 gccgggcatg accagccaca gcctggtgcc gatggcagca cgggtggccg gcattgaatt
     1801 tgatgcgctg tgcctgcgca tcctggacgg cgcgcatgtg ggataaccac cggttgctgc
     1861 gcggcgtcgc caacctgttg ttgctggcgt cgatcctgac gctgctggtg gcgggcgcgc
     1921 agtgggtggt gaggagcggc ttgctgccga tccgtgcggt gcatatccgc agcaagctta
     1981 cccacgtgac gcaggagcag ttgcgttatg tggccgagca cgagctgaaa ggctcgttct
     2041 tgacggtgga tatcaccgaa acccgtgcgg cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2161 nnnnnnnnnn ngtgctgccg gaagaactga aactgtcggc gcggcgcgcc tggcaggtca
     2221 agctggacaa tagtgtgatc ctggagttgg ggcgccagga catcggcctg cgggtgggcc
     2281 gctttgctgc cagctggcgc gatcaactgt cgcgcctgtc gtacaagatt gaatatgtgg
     2341 atttgcgcta ccccaacggc tttgccgtgc gcatgccgga ctacaagccg ggcaagggcg
     2401 ccccggccat gccgcgtccg gcggcctgaa tgaatacgaa agcttaacgg agcacgtggt
     2461 gagcaaaccg agagaaccta aaaacctgct ggtcgggctg gatgtgggca cgtccaagat
     2521 tgttgccgcc gtggccgagg tgctcgagga tggcgcgctg aacatcatcg gcatgggtca
     2581 gacatcgtcg cgtggcctga aaaagggcat ggtggtgaat atcgaatcca ccgtgcaagc
     2641 cattcagcgt gcagtggaag aagccgaact gatggctgac tgcaccattc accaagtatt
     2701 taccgggatt gccggcagcc atattaaaag cgtaaattct cacggcatgg tggcgattcg
     2761 cgagcgtgaa gttacccagg ctgatattga tcgggtgatc gagacggcgc gggcggtgac
     2821 cacgccgccg gatcaccaag tgctgcacat cctgacccag gagttttcca ttgacggtca
     2881 ggaagacgtg cgcgaaccgc tgggcatgag tggcgtgcgc ctggaaaccc gggtgcacat
     2941 cgtcaccggg gcggttagcg ccgcgcaaaa tattttgaag tgtgtgcgac gttgcaatct
     3001 ggacgttgcc gatctgatcc ttcagccact ggcatcggcc acggcggtgt tgactgacga
     3061 cgagcgcgat ctgggcgtat gcttgatcga catcggcggc ggcaccaccg atattgccgt
     3121 gtacacccgc ggggcgattc gccacacggc ggtgataccg attgccgggg atcagatcac
     3181 caatgacatt gccatggctt tgcgtacgcc aaccaaagag gccgaagata tcaagctggt
     3241 tcacggttgc gcgttgatgc aactggcgga tccggcagat atgattgacg tgcccggcgt
     3301 gggcgagcgc gggccgcgcc agctgtcgcg cgccaccctg gcagaagtga tcgagccgcg
     3361 tgtggaagaa ttgttccaat tgattcaaca ggaactgcgc cgcaccggtt ttgaagatgt
     3421 gctgtcctcc ggcatcgtat tgaccggtgg ggcagccaaa atgcccggca tggtggaact
     3481 ggcggaagaa gtattccaca tgccggtccg ggtgggggta cccaaatact tcggcgggct
     3541 ggccgaagcg gtgaaggatc cccgcttttc cacgtcgatc ggcttgttgc tgatcggcaa
     3601 ggatcagttg caaaaaaccg caggtccaaa acccgaaacc gcctcggtgg gtggcctgtt
     3661 ctcgcggatg aaagcctggt tcgccgggaa tttctgacgc acgcattatc gttgtagcga
     3721 cattttttgt tttatagggc agtcgtcaac gcagtttttc gaggagagaa ccgtcatgtc
     3781 ttccaacatg gtctttgaga tggtgcagga aatcgcaccc gaagccgtca tcaaggtgat
     3841 tggcgtcggg ggtggtggct gcaatgccgt cgataacatg atcgaaagcc aggtcaaggg
     3901 cgttgaattc atcgccgcca ataccgacgc ccaggccttg cgccgcaacc gtgcgccggt
     3961 gcagattcaa atcggccaga ccctgaccaa gggcttgggc gcaggcgcca acccggatgt
     4021 cggccgcagc gccgccatcg aagaccgcga ccgcatcgct gaagtgctgc gcggcgccaa
     4081 tatggtgttc atcgccaccg gcatgggcgg cggcaccggc accggcgctg cgccggtgat
     4141 tgccgaaatc gccaaggaaa tgggcattct gaccgtcggt gtggtcactc gcccgttctc
     4201 gcacgaaggc cgtcgccacc aagtggccgg tgcgggcatc gaagaactga aaaaatacgt
     4261 cgattcgctg atcgtcatcc ccaacgagaa gctgctgcag gtgctgggct cggatgtgac
     4321 catgcgccag gctttccgcg ccgctgacga tgtgctgcgc ggcgccgtgg ccggcatcgc
     4381 tgaagtgatt acctgcccgg gcttcatcaa cgtcgacttt gccgacgtca agaacgtgat
     4441 gggccagatg ggcatggcga tgatgggctc ggccgtggct tccggcctgg atcgctcgcg
     4501 cattgccgct gaagaggctg ttgccagccc gctgctggac gatatcagtc tggacggcgc
     4561 acgcggcgtg ctggtgaata tctccaccgc tccgggctgc ctgctgctgc gtgaatacca
     4621 tgaaatcatg gaaatcatcc gcaactacgc ccacgccgac gccgaagtga aattcggcac
     4681 tgccgaagtg gacggcatgt cggaagacga aatccgtgtg accttgatcg ccaccgggct
     4741 gggcggcggc aagaaacctg cacgcgaaga acgtcgcgag caactgaaag tggtgaagaa
     4801 cggcaccgat ggcaccacca tggaagtgcc ggacttccaa cagccggatt tcgacaaacc
     4861 cagcgtgttc cgcgcccgtt cgggccgtgg cgcgccgcag caggtggatt tctctaatcc
     4921 ctcggtgggc gattcgttcg atattccggc tttcctgcgc cgccaggctg attgaagctg
     4981 actgtgtgtg cgtgcggcgt aggctgcgcc gcgcgaacac gccccggttt cgcgcctgtc
     5041 cgcttggtgc gttgcctcga caacgcatga cggctgcccg cctttcgcgg gcaggcgcgg
     5101 atttttattg ccttcggaac tttgatagtg ctgggctatc agagtcggca agacaatcaa
     5161 tcagccggtc aattcggctg tgttagactt catcacatcg tttcgccccc tgtcgccaga
     5221 agggacccgg catgattcac cagcgcacac tcagccaaac tatctccgcc agcggcgtcg
     5281 gcctgcactc cggcgaacgg gtgaaactca ccctgcgccc cgcgccgccc gatactggca
     5341 tcatctttcg tcgcaccgac ctcaccccgc cggttgaact gcgcgccgaa cccgcgctgg
     5401 tcaacgacac ccgtctgtcg tccaccctgg tggacccaaa cggcgtgcgc gtggccaccg
     5461 tcgagcacct gatgagcgcc ctggccggcc tgggcatcga caacctgatt gtagaaatga
     5521 ccggcccgga aacgccgatc atggacggca gcgccgcgcc gtttatctat ctgctggaca
     5581 gcgccggcat cagcgagcaa accgcgctca agcgctatat ccgcgtgctc aagccgctgg
     5641 aagtggtgga aggcgacaaa tgggtgaagc tcaccccgta tgacggcttt actgtcagcc
     5701 tggaaatcga attcaatcac ccggtgtttc gccgcacccc gcaacacctg accatcgact
     5761 ttgccgcaca atcgtatatc gacgaagtca gccgcgcgcg cacctttggc tttatgcacg
     5821 aagtcgaata catgcgcatg aacggcctgg ggctgggcgg caatctggac aacgccatcg
     5881 tgatcgacga cgactcggtg ctgaacaaag acggcctgcg ctttaacaac gaatttgtcc
     5941 gccacaagat tctcgacgcc atcggcgacc tgtacatgat tggccacccg ctgattgccg
     6001 ctttttccgg cttcaagtct ggccacgcca tgaacaacaa gctgctgcgc gaattgcagg
     6061 cgcggccgga cgcctgggaa tacgccacct tccgccgccc cagcgacgcg ccgtccagct
     6121 tccatcgcct gccccaagcc gcctgagcgg atttccccct gtttgccgcc ggcgtctttg
     6181 ccggcggcct gattgattgc aaggagcgcc catgtcggca ctgactcact tcaacgccga
     6241 cggccaggcg catatggtcg atgtcggcga caaagccgac acccaccgcc gcgccatcgc
     6301 cagcggcgtg atccgcatgc agccggacac ctttgctctg gtgcaaagcg gcagccacaa
     6361 aaaaggcgac gtgctgggca ttgcccgcgt cgccgccatc atggccagca aacacaccag
     6421 cacgctgatt ccgctgtgcc acccgctggc gctgagccat gtcgatgtcg aatttcagct
     6481 ggacgccgct gccagcgcca tcacctgccg ggtgactgcc gagaccgttg gcaaaaccgg
     6541 cgtggaaatg gaagcgctga ccgccgtgca aatcggcctg ctgaccatct acgatatgtg
     6601 caaggcggtg gatcgcggca tggtgatcgg tgaagtgcgt ctgctggaaa aacaaggcgg
     6661 caagtcgggg ctgtggcggg cggaaggcct ggattagcaa tacggtaaac cagtgccctg
     6721 gcgcacagca aaacggcggc tcctgactgg gccgccgttt tgctgtggca agacaagcgc
     6781 tgctacacgc tcaccatatt atcccggtga atcagctccg gttcgcccat atagcccaga
     6841 atggattcga tctcacgggt ggcgtggcgc atgatcagcc gggtttcgtc cgagctgtag
     6901 ttgaccagcc cgcgcgccac ttcgtggcct tgcggatcga cgcaggccag cgcttcgccg
     6961 cgcaggaact ctccttccac cgccaccacg ccaatcggca gcaggctggt gcccttgtgc
     7021 acgacggcat gggcggcgcc ttcgtccagc accaggcggc cggccagttg cagatgatcg
     7081 gccagccatt gtttgcgcgc cagcagcggg gtgtcggcgg caatcagctg ggtgccaatc
     7141 gcctcgccgt cggccagccg cgacagcaca ttggcttcgc gcccgcaggc aatcaccgtg
     7201 gcggcgccgc tgcgcgcggc gcgcttggcg gcgatgatct tggtgatcat gccgcccgtg
     7261 cccactgagc tgccggcgcc gccggccatg tcttccagcc gcgggtcgcc cgcctgcgcc
     7321 tcgtgcacaa actcggcgtc ggcgtgcttg cgcgggtcgg cggtgtacaa accttgctgg
     7381 tcggtgagga tgatcagcgc gtcggcttca atcagattgg tgacgagcgc gcccagggtg
     7441 tcgttgtcgc caaagcggat ttcgctggtg gccaccgtgt cgttttcgtt gatgatcggc
     7501 accacattca gcttgagcag ggtgatcagc gtcgagcggg cgttcagata gcgggtgcgg
     7561 tcggccaggt cttcgtgggt gagcagcacc tgggcggttt tcaggccttc ggcgcgaaag
     7621 gcggtttcat acgcctgcac cagccccatc tggccgacgg cggcggcggc ctgcaattcg
     7681 tgcaccgcct tgggtcgggt cgcccagccc agccgttgca gcccttcggc aatcgcgccg
     7741 gacgacacca gcaccacttg cttgccccgg cgggtcaggt cggcgatttc gctggcccag
     7801 cgcgacagcg cggcgtggtc cagacccttg ccatcgttgg tgaccagact ggagccgacc
     7861 ttgacgacaa tgcgctggct ggcgtggatg acggactgca taaggccatt cctgaaaatt
     7921 gcggtgcgcc attatacaaa gctggcgcag gctgagggga ggcttgcgcg cgccgcgtgt
     7981 gcggatgggg atggccgttt tcggcgcttg tttgccaagg ccgcgcccgc tatgcttttg
     8041 cgctgtcatg atggcgccct gcgccacgga gcttgcatgg agctgaccac tgccatccct
     8101 accctggctc acgccattca acaatccgtt gccccggtat tcctgctgac cggggtaggc
     8161 tcactgctgg gcgtgctggt caaccggctg gggcggattg tcgatcgcta tcgacagttg
     8221 cgcagcctgc cggctgaaca gatcaccccg catcgggccg agatggcgct gctgcaacgg
     8281 cgttcgcggc tgattcactt tgccattcgc tacagcacac tgtgcgcttt gctgatttgt
     8341 gtggtgattt ccttgctgtt cattggcgtt gagctgggca gcgagctgtc gccgctgatt
     8401 gctgcgctgt ttgtctgcgg catgctgtcg ctgattgcca gcctggtgtg ttttctcaag
     8461 gaaatttctc tggccacctg gcggctggag gcagcgtttg atgcagattt gcgccaggac
     8521 accccaccgc cgcccgacgg ccagtcttag ccccgccttt ggggtggacg gcgcgcccat
     8581 gtttgatggg ttaaccgtgc cgggcgcggc tcacggcacg gcgggctctt gttcgccagc
     8641 ctgaccgttg ccatcgccca ccagcgcatc cagcgtgcgc aaggtggcgt tcaccagttg
     8701 cgccatctgt tctgcctcaa tcacatacgg cggcatgaaa tacaccgtct ggccaatcgg
     8761 gcgcagcaag cagccttgct ccagcatggc gctgaaaaac gccagggcaa agtcgctgcg
     8821 cgtggtggcc acgtcaaacg cccagatcat gccctgctgg cggaagtgac gcaccgccgg
     8881 gtgcgcttgc agcggggcga gcagcggggc aaaggcggcg gccttggcgc ggttttgcgc
     8941 cagcacgtcg tcttcggcaa agatgtccag caccgccagc gcggcgcggc aggccagcgc
     9001 attgccggtg tagctgtgcg aatgcaagaa gccgcgacgg atgtccgggt ggtaaaacgc
     9061 cgcatacacc tcgtcggtgg tcagcacgca ggacagcggc aggtagccgc cggtgatgcc
     9121 cttggacaaa cacaaaaagt ccgggcgcac gccggcctgc tcgcaggcga acagcgtgcc
     9181 ggtgcggcca aagccgacgg cgatttcgtc agcgatcagg tgcgcgccat attggctgca
     9241 cagccggcgc gcctcggcca ggtacagcgg gtggtgcatg gccatgccgg cggcgccttg
     9301 caccagcggc tcgataatca gcgccgccac ctcgccagcg tgttgctgca acagtgcttc
     9361 cagcccggcg gcggcgcggc gggcgacggt ttcggcggtt tcgccggggc cggccagccg
     9421 cgcatcgggg ctgggcgcgg tcagcgcggc gttcagcagt ggggcatagg tctggcggaa
     9481 cagcggcacg tcggtgaccg ccagcgcgcc cagggtttcg ccgtggtagc tgtgttccag
     9541 gtggataaag cggctttttt gtggggcgcc gctttgctgc cagtactgaa aactcatttt
     9601 cagcgcgatt tccgtcgcgc tggcgccgtc cgagccgtaa aatgcatggc ccagcccgga
     9661 cagggcggcc agccgctcgg acaactccac caccggctgg tgggtaaagc cggccagcat
     9721 cacatgctcc agccggccca gctggtcgat cagcgcggcg ttgatgcgcg gattggcgtg
     9781 gccaaacaga ttgacccacc acgagctgac tgcgtccaga taacgcttgc cgtcaaagtc
     9841 gatcagccac accccttcgc cacgggcgat gggaatcagc ggaaaggttt cgtggcgctg
     9901 catttgcgtg caggggtgcc agacggcgcg ggtgctgcgg ctgagccaat cagtgctggg
     9961 catgacaaat ccggggcaga acgtaaaaca ggcagtctaa gcagtccggc ggcggcaggc
    10021 aaacctgcgc ggtgggcgat tcatttcagg aaagtggcat gaagcaggtt ttcagggcgc
    10081 ggcgggcaac gccggcgcaa cagggcttga ccctgattgg tcagatgatg gtgttgctgg
    10141 tagccggcgc gctgggcgcg gtggccttgt cgtcgtggct ggatgtgcag gtgcgcgagc
    10201 gcgccaccga ggcgtacaac cagctggagg ccatcaagcc tgcggcagaa aaactcggcc
    10261 gcgagcaacg cggccaaccg tggccggcca gcctggccat ggccacattg ggcgaagcgc
    10321 cggcaggcag ccgctttgtg gctggcagct ggcaggccag cgcccaggac cggctgctga
    10381 ccgtgtcggt gcaattgcag gacaccggcc gccatctgga cggccagcgc ctgctgctca
    10441 agggctggct ggaagaggat ggcgatgtgc gctgggtgtg cgccagcgat gtggacccgc
    10501 gctggcataa gcaactgccg gcgccgtgtc agtacaagcc gagccgttcg ccggagccga
    10561 aatccgaata aatgtggcgc cttgtggcgt ctcttgagcc ttgacccagc gccggactat
    10621 ccggcgcttt ttgttgtcag ggtggcgcta tccctggcta aggctgatca agcctggtga
    10681 taaatgacaa ttgctgacga caacgggttg gtgacttgct cgatgattca atataaatca
    10741 tactcggatg tgataaaaac ctaaattgtg ctgacatagc ctgtcagtcc gttgtggttt
    10801 actgcttcac atgtcagatt tttccaagcg gactgtttca tgtcagttga tccacccaaa
    10861 cacgaaatgc tggccaaccg gctggcggaa atcctgctca agctcaatct ggacgaacgg
    10921 cccagccgcc aggaactggc cgcagagttc aaggtaaccg aacgaaccat ctaccgcgac
    10981 ctgaaccgcc tgggcagcat tgtggaacaa ctgccggatg ggcgttacca gctggccagc
    11041 gaataccggg gcaagctcaa gcccaaggac ctgcaatcct tcgcccagct ggccggggtg
    11101 gaacagctgt ttccggatgc cagcccgcag tttctggtgg ccctgctcga taccctgtgc
    11161 cagggcagcc tgctggtgca tggccatcaa tacgaacgtt tcaaaccgca cgacaaaagc
    11221 tttgacctgc tgaacgaggc ggttaccgga caacgaatct gccggctgac ctatcgcgac
    11281 aagccccgcg aactgcaccc ctaccggctg atcaacaaaa acggcatctg gtatctggcc
    11341 gccaccgagc aaggccagct caaggcgttt gccttcagcc ggatcagcca actgagcgtg
    11401 actgagcaga cctttacccc cgatgccacg attcaggcca atatcgaaaa cgaagacgat
    11461 atctggttca gccgggaaaa aaccgaagtc ctgctcagca tcgcgcccga aattgcctac
    11521 tacttccgcc gccgcaagct gctgcccaag caggagctgg tgcgcgagct ggaaagtggc
    11581 ggcttgatcg tcagcagcca gatcagccat gtagaccaga ttctgccgct ggcccagttc
    11641 tggctgccgc gcatccgggt ggttgagccg gcctggctgc gcgaacgact ggaaacggta
    11701 ttgcgcgact atctggagca gaagtgacca tgccgctgcc tgcgcgcccg ttgctttatc
    11761 agtgcccggc ttgcggcttg cggcagattg tgacacccat cggcgatgtg ctgctgggcg
    11821 agccggctgc ctgcctgcat
//
LOCUS       DAOPYX010000054        11676 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_6264_length_11676_cov_11.617331, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000054 DAOPYX010000000
VERSION     DAOPYX010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 11676)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11676)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11676
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6264_length_11676_cov_11.617331"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1087)
                     /locus_tag="PKN55_04365"
     CDS             complement(<1..1087)
                     /locus_tag="PKN55_04365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012698275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HNX70508.1"
                     /translation="MTPPILPSLPPAPHRVPHWFALSYLGALVVVSLYPFTEWEFNGQ
                     PWLDFLLYPLPYYRTAFDQGVNVLAYVPYGVALARMFRPAWLGVLAALGLGALTSLVI
                     EIIQLYLPMRVASNLDVLCNAAGALAGGLLATLPWTVRLGRAMQRLRRRWLLADNSAD
                     YALMLVALWFLTQINPANPLFGVVVMPEGLPQPFESPIADPALFLFLLEAGGAMLNLT
                     ATLLFIASFLARRRDYAPVLGGFFLLAWLFKVAVAAAMLKPVAYFAWINPHVLAGLAL
                     GGVLTWLLCHTQRLVQSLCALLLLGLAQLAAAQWPLVAADDDQRGLFRWAYHLTNINA
                     LSEFANRLWPWAALACVLLSISRQLRRE"
     gene            complement(1146..2210)
                     /locus_tag="PKN55_04370"
     CDS             complement(1146..2210)
                     /locus_tag="PKN55_04370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011137646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HNX70509.1"
                     /translation="MKKLLLSMSLLASSMAFAADELHLYNWNNYLTEATAKRFEQSCK
                     CKLVQDYYGDNEEMLAKLAAGAKGYDIVVPTGFAVQTLIGQKGAAPLDKAQLSNLKNI
                     NPGYMGGFFDKTNTYSVPYAFTTTVLGYNETQLQKAGVLDKANSWALIFDPAVVSKIK
                     GKVTVLDSQRELMAAALMYLGYPANETDPAKWKQAQQVILKAKPYWAAFNNQSYIKEL
                     TVGNAWVSLGYSNDLFQAQQDALKAKRPFKLNFSLQQEGNTLSMDNLVILKDAPRKDL
                     AHKFINFMLDGKNAAELTNEVGAGNPNQAAMPNVKPEITKLSAIFPSKEALPKLQQLQ
                     DLNGKQRRELNKLWSEIKLK"
     gene            complement(2460..3236)
                     /locus_tag="PKN55_04375"
     CDS             complement(2460..3236)
                     /locus_tag="PKN55_04375"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012698120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNX70510.1"
                     /translation="MAKRQSWWLWAAAALAYVFLYLPLVIVVVYSFNDSKLNAEWVGF
                     TLDWYHKLFNNREMLVAARNSMVIGVVSAVLATFFGALAGIAMHRYKLRLLPFLVLTP
                     IAIPEILMGVSLLIFFVLLNVTLGMTSIILAHVAFCIGFVAIVVRARMQGMDNSLVEA
                     ARDLGATPWQAFRLVTLPQIMPGILAGGLMAFTLSIDDFVITFFTAGVGSSTLPLQIY
                     SMIKIAVTPEVNAISTLLMVLTLCLIVLVSKVSPGALRAE"
     gene            complement(3229..4161)
                     /locus_tag="PKN55_04380"
     CDS             complement(3229..4161)
                     /locus_tag="PKN55_04380"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011137648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNX70511.1"
                     /translation="MQERLGKWLLSWPPLAYLVVFFLLPSLIMMFAAFRYPGDYGGLM
                     PLTSEDPATGKLVLDITLDNWRDYLTLESFVRLVDEPLYIELFIKSLWYATLTTLICI
                     AMGYPLAWLIARSPKKYRDLMLLLVILPFWSNFLIRIYAWMILLGPQSALSKAINLVV
                     TAFGHEPVSLLFTPFAVIVCLVYVHLPFMVLPLYANLEKHDMALLDAAQDLGASAWQR
                     FWKITWPLSLPGVYAGAALVFIPALGMFAVPDIIGGTEGIMIGNLIKQQFLETRDWPF
                     GAVLSIMLTGGVLLMAGLAAAVGRGKQKAKQKHG"
     gene            complement(4165..5253)
                     /locus_tag="PKN55_04385"
     CDS             complement(4165..5253)
                     /locus_tag="PKN55_04385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011137649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNX70512.1"
                     /translation="MALLEIRNVVKRFGDFTAVNHVSLSVNAGEFFTLLGPSGCGKTT
                     LLRMLAGFEQPDSGEILLDGKDVSNVPPEKRPVHTVFQSYALFPHMTVRENIAFPLKM
                     AGWSADKIAPQVDELLEDVRLSKFGKRYPHELSGGQRQRVAIARALVDRPRLLLLDEP
                     LSALDAKLREEMQIELINLQKEVGITFVYVTHDQGEALALSHRIAVMNSGQVEQLDMP
                     ETIYSYPRNRFVADFIGQCNVLESEVKAIHGDGTMTIAIKGCGEMKALAREGVTVGQQ
                     GWLALRPEKIKLDRELPELANEAYFKGRVHDCLYMGDVTLYIVEVGEGVRIEAMLPNS
                     APGLAKLFDDNDLVEIAWRFDAGSFLTE"
     gene            complement(5697..6833)
                     /gene="obgE"
                     /locus_tag="PKN55_04390"
     CDS             complement(5697..6833)
                     /gene="obgE"
                     /locus_tag="PKN55_04390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012698411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HNX70513.1"
                     /translation="MKFIDEARIEVCGGKGGNGSASMRREKFVPRGGPDGGDGGRGGN
                     VIAVADKDINTLVDYRFVKKYIARNGENGRGADCYGKGADDIILRMPVGTLITDSLTG
                     EVVADLTTDGMEVIIARGGKGGLGNIHFKSSVNRAPRQFTHGEPGEQRELKLELKVLA
                     DIGLLGMPNAGKSTFIRSVSAARPKVADYPFTTLHPNLGVVRIDENRSFVIADIPGLI
                     EGAADGAGLGHRFLKHLQRTGLLLHIVDLAPFDPDTDPVAEARAIVEELRKYDESLYN
                     KPRWLVLNKLDMVDDDERTARVQQFLADYGWQENPDPYADFDPMAPRHFVISALTGEG
                     TRELTYAVMDYLDTLRQRAEQDRLAAAAAARPGELIAPSQPQAD"
     gene            complement(7001..7279)
                     /gene="rpmA"
                     /locus_tag="PKN55_04395"
     CDS             complement(7001..7279)
                     /gene="rpmA"
                     /locus_tag="PKN55_04395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007341687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HNX70514.1"
                     /translation="MAHKKAGGSSRNGRDSESKRLGTKAYGGELIPAGSIIIRQRGTR
                     FHAGDNVGMGKDHTLFAKVDGYVKFEVKGAAKRKTVTVLPYTGEAEAA"
     gene            complement(7295..7606)
                     /gene="rplU"
                     /locus_tag="PKN55_04400"
     CDS             complement(7295..7606)
                     /gene="rplU"
                     /locus_tag="PKN55_04400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011134403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HNX70515.1"
                     /translation="MYAVVKTGGKQYRVTIGEKLKVEQIPADIDSQIVLDQVLMVADG
                     DQVAVGTPLVAGATVTATVVSHGRGEKIRIFKMRRRKHYQKHQGHRQNYTEIRIDAIS
                     K"
     gene            7879..9939
                     /locus_tag="PKN55_04405"
     CDS             7879..9939
                     /locus_tag="PKN55_04405"
                     /inference="COORDINATES: protein motif:HMM:NF012602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HNX70516.1"
                     /translation="MPPIQRPSVCPHDCPSVCALSVEVGDDGRIGRVRGAAQPYTDGV
                     ICAKVARYAERVHHPERLTQPLLRVGDKGSGQFTPISWDAALDLLAERIVSATARHGA
                     QSIWPYHYAGTMGVVQRAAIRRLGHLAGWSRQRETFCVALSDAGWLAGVGAKRGLDPR
                     EVMESELIVVWGGNPVHTQVNFMHWLQKARRGRDVPLVVVDPYRTATAAKADVHLALR
                     PGTDGALACAVMHVLLAEGLADRDYLAKHTDFSPEVEAHLASRTPAWAAEITGLSVEQ
                     IVQFARLYGGARRSFLRVGYGFSRQRNGAAAMHAVSCLPAVTGAWQWPGGGALYSNGG
                     LYGLNTRALYGLDAVTPDTRVLDMGRLGAVLAGERRDLGDGPPVSVLLVQNTNPAVVA
                     PDSLAVRRGLLRDDLFVCVHEQFMTDTARLADLVLPATTFLEHDDLYQASGHTFLQAA
                     RALIAPLGECRANHDFISQLAARLGVSHPAFQQDAWALVDQVLRESGQPDADSLYAAG
                     WHDCTPGFARAHFLEGFGHADGRFRFAPDWAARGEGHASMPRYPDHQAVIDAASAERP
                     FRLVAAPARHFLNSSFTETPSSRQLEGRPTAQLHPDAAARLGIADGELIRLGNAQASV
                     LLHARTVAGVQPDTVVVESQWPNDAFIEGVGINALVSAEPGFPAAGVAYHDTAVWVQR
                     VAGE"
     gene            9971..10228
                     /locus_tag="PKN55_04410"
     CDS             9971..10228
                     /locus_tag="PKN55_04410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX70517.1"
                     /translation="MRILPVFAPLRLNAAMRGLLERVLPSAGFLSFLLYIVWTEWQAA
                     PWPAAIGLTLAAGLTLFTRTGLIGATALGSVVYFACGLVWR"
     gene            10425..>11676
                     /locus_tag="PKN55_04415"
     CDS             10425..>11676
                     /locus_tag="PKN55_04415"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNX70518.1"
                     /translation="MTAAAPRPWPAPIRALGHAHFRRYFAGQAVSILGSWIQQVAMAW
                     LVYRLTGSAALLGVTAFAALIPQLLVGPFAGAWVDKHDKRRALLWVQALLTVQALALA
                     ALTWLGWVSPWLLVAMSGLLGVLNSFDTPLRQSLIGSFVGRREDLPNALALNAMLFNS
                     GRFIGPPIAGALLAALSEAACFALNALTFLALIIAVASIRMPAPPKAQGSMGDVFREG
                     VSYAWHTWLVRLLIFTLIALNLTASAYAVLLPVFARDVFAGDARTLGWLWGAAGCGAF
                     VSTLYLATRQSMPGLVRSIVWGAALSALALLVFGLTAWLPLALAAMAALGFGISVSNV
                     GINMLLQSTAPDRLRGRVVSFFTSARFGFDALGGLLAGLLATALGAGWTLALEGVLLL
                     GFVLFLLSRRRALRQAIDRHDPTAH"
BASE COUNT         2038 a         3743 c         3718 g         2177 t
ORIGIN      
        1 gctcgcggcg caactggcgg ctgatcgaca acagcacgca cgccagtgcc gcccacggcc
       61 acagccggtt ggcgaattcg gatagggcgt tgatattggt caaatggtag gcccagcgga
      121 acaggccacg ctggtcgtcg tcggcggcca ccagcggcca ttgtgccgcc gccagttgcg
      181 ccaggcccag caacagcagc gcgcagaggc tttgcaccag ccgctgggtg tggcacagca
      241 gccaggtcag cacgccgccc agcgccaaac cggccagcac atgcgggttg atccaggcaa
      301 aatacgccac cggcttgagc atggcggcgg ccactgccac cttgaacaac caggccagca
      361 gaaaaaaccc gcccagcacc ggcgcataat cgcgccgtcg cgccagaaaa ctggcgataa
      421 acaacagcgt ggcggtcagg ttcagcatgg ccccgccggc ttccagcaga aacaaaaaca
      481 aggctggatc ggcaatcggt gattcaaacg gctgcggcag cccttccggc atcaccacca
      541 cgccaaacag cgggttggcc gggttgatct gggtgagaaa ccacagcgcc accagcatca
      601 aggcgtagtc ggcgctgttg tcggccagca gccagcgccg gcgcagccgc tgcatggcgc
      661 ggcccagccg cactgtccac ggcagcgtag ccagcaagcc gccagccagc gcacccgcgg
      721 cattgcacag cacgtccaga ttggacgcca cccgcatcgg cagatacagc tggatgattt
      781 caattaccag gctggtcagc gcccccaggc ccagcgccgc caacacgccc agccaggccg
      841 gccggaacat gcgcgccaag gcgacgccat acggcacata ggccagcaca ttgacgccct
      901 gatcaaaggc agtgcggtaa tacggcagcg gatacagcag gaaatccagc cagggttggc
      961 cgttgaattc ccattcagtg aacgggtaaa gactgaccac caccagcgca cccagataac
     1021 tgagggcaaa ccagtgcggc acccggtgcg gggcgggcgg caaagaaggc aggattggcg
     1081 gcgtcatggc tgtgcgccgc ccgcccgatg ccgggcgagc ggactgcgcc agaagcggga
     1141 gagtattact tcagcttgat ttccgaccac agcttgttca actcacgacg ttgcttgccg
     1201 ttgaggtctt gcagctgttg cagcttgggc agcgcttcct tgctcgggaa gatggccgac
     1261 agcttggtga tttccggctt cacgttcggc atggcggcct ggttggggtt gccggcgccc
     1321 acttcgttgg tcagctcggc ggcgttcttg ccatccagca tgaagttgat gaacttgtgc
     1381 gccaagtcct tgcgcggggc gtctttcagg atcaccaggt tatccatcga cagggtgttg
     1441 ccttcttgtt gcaggctgaa attcagcttg aacgggcgct tggccttcag cgcatcctgc
     1501 tgggcctgga acaggtcgtt ggaatacccc agcgacaccc aggcgttgcc cacggtcagc
     1561 tctttgatat agctctggtt gttgaacgct gcccagtacg gcttggcctt cagaatcacc
     1621 tgctgggcct gcttccactt ggccgggtcg gtttcgttgg ccgggtagcc cagatacatc
     1681 agcgcggcgg ccatcagctc gcgctgcgaa tccagcacgg tgaccttgcc cttgatcttg
     1741 gagaccacgg ccggatcaaa aatcagcgcc cagctattgg ccttgtccag cacgccggct
     1801 ttttgcagct gggtttcgtt atagcccagc accgtggtgg tgaaggcgta cggcaccgaa
     1861 taggtgttgg tcttgtcgaa gaaaccgccc atataacccg ggttgatgtt cttcagattg
     1921 ctcagctgcg ccttgtccag cggcgcggcg cctttctggc cgatcagcgt ttgcacggca
     1981 aaaccggtgg gcaccacaat atcgtagccc ttggcgcccg cggccagctt ggccagcatc
     2041 tcttcgttgt cgccgtagta atcctgcacc agcttacact tgcacgactg ctcaaagcgc
     2101 ttggcggtgg cttcggtcag gtagttgttc cagttgtaca ggtgcagctc atcagcggca
     2161 aacgccatgg aactggccag caaggacatc gacagcagca gctttttcat cttgttccct
     2221 ttacggttcg gtcaaaatca agtcacacac actgcacacc tgcttgctgg ctggccggca
     2281 aacaggcaga aattatccca ctcacgcgcc atcgtgccca ggcgggcatc cagaactcgc
     2341 cgccgcgtgg cgcgggtgaa ggctcaactc caccgtcacc ctcagcgcgc cgtggccgct
     2401 tgccaggggg cgacgcagtg agaaacaaaa cggcgatggt gaagcaccga tgcggcccat
     2461 cactccgccc gcaaggcccc cggcgacacc ttggacacca gcacaatcaa acacaaggtc
     2521 agcaccatca acagcgtcga aatcgcattc acctctggcg tcaccgcgat cttgatcatc
     2581 gaataaatct gcaaaggcag cgtcgatgag cccaccccgg cggtaaagaa ggtgatcaca
     2641 aaatcatcaa tcgacaaggt gaacgccatc aggccgccgg cgagaatgcc cggcatgatc
     2701 tgcggcagcg tcaccagccg gaaagcctgc cacggcgtgg cgcccaggtc gcgggcggct
     2761 tccaccaggc tgttgtccat gccctgcatg cgcgcgcgca ccacaatcgc cacaaagccg
     2821 atgcaaaacg ccacatgggc caggatgatc gaggtcatgc ccagggtgac attcagcaac
     2881 acaaagaaaa tcagcaggct cacgcccatc aggatttccg ggatggcgat cggggtcagc
     2941 accaggaagg gcagcaggcg cagcttgtag cggtgcatgg cgatgccggc cagcgcgcca
     3001 aaaaaggtgg ccagcacggc ggacaccacg ccaatcacca tcgaattgcg cgccgccacc
     3061 agcatttcgc ggttattgaa cagcttatgg taccagtcca gggtgaagcc cacccactcg
     3121 gcgttgagct tggaatcgtt gaacgaatac accaccacga tcaccagcgg caggtacagg
     3181 aacacatagg ccagcgccgc cgccgcccac agccaccagg attgtcgttt agccatgttt
     3241 ctgcttcgcc ttctgtttgc cgcgccccac ggcggcggcc agacccgcca tcagcagcac
     3301 gccgccggtc agcatgatcg acagcacggc gccaaacggc cagtcgcggg tttccaggaa
     3361 ttgctgcttg atcaggttgc cgatcatgat gccctcagtg ccgccgatga tgtccggcac
     3421 ggcaaacatg cccagcgccg ggataaacac cagcgccgca ccggcgtaca cgccaggcag
     3481 cgacaagggc caggtgatct tccagaagcg ctgccaggcc gaagcgccca aatcctgcgc
     3541 agcgtccagc aaggccatgt cgtgcttttc cagattggcg tacagcggca acaccatgaa
     3601 tggcagatgc acatacacca ggcagacaat caccgcaaac ggggtgaaca gcaggctgac
     3661 cggctcgtgg ccaaacgccg tcaccaccag attgatggcc ttgctcagcg ccgattgcgg
     3721 gcccagcagg atcatccagg cataaatccg gatcaggaaa ttcgaccaga acggcagaat
     3781 caccagcaac agcatcaggt cgcggtattt tttcgggctg cgcgcaatca gccaggccag
     3841 cgggtagccc atggcgatgc aaatcagcgt ggtcagcgtg gcgtaccaca gtgatttgat
     3901 gaacagctcg atatacagcg gctcgtccac cagccggaca aagctttcca gcgtcagata
     3961 atcgcgccag ttatccaggg tgatgtccag caccagcttg ccggtggccg ggtcttccga
     4021 ggtcagcggc atcagcccgc cgtaatcgcc agggtagcga aaggcggcaa acatcatgat
     4081 cagcgacggc agcaagaaaa acaccaccag atacgccagc ggcggccacg acagcagcca
     4141 ttttcccaaa cgttcctgca tggcttactc cgtcaggaag ctgccggcat caaaacgcca
     4201 ggcgatttca accagatcat tgtcgtcaaa cagcttggcc aggcccggcg ccgagttggg
     4261 cagcatggcc tcgatgcgca cgccctcgcc cacttcgacg atgtacagcg tcacatcgcc
     4321 catgtacaga cagtcatgca cgcggccctt gaagtaagcc tcgttggcca gctcgggcag
     4381 ttcgcggtcg agtttgattt tttccgggcg cagcgccagc cagccttgct ggcccaccgt
     4441 cacaccttcg cgcgccagcg ccttcatttc gccgcagccc ttgatggcga tggtcatcgt
     4501 gccgtcgccg tggatggcct tgacttcgct ttccagcaca ttgcactggc cgatgaaatc
     4561 cgccacaaag cggttgcgcg ggtagctgta gatggtttcc ggcatgtcca gctgctcaac
     4621 ctggccgctg ttcatcaccg caatgcggtg cgacagcgcc agcgcctcgc cctggtcatg
     4681 ggtgacgtag acaaaggtga tgccgacttc tttttgcaga ttgatcagct cgatctgcat
     4741 ttcttcgcgc agcttggcgt ccagtgccga cagcggctcg tccagcagca agaggcgcgg
     4801 gcggtccacc agcgcgcggg caatcgccac ccgctggcgt tggccgccgg acagctcgtg
     4861 cgggtagcgt ttgccgaatt tgctcaggcg cacgtcttcc agcagttcgt ccacttgcgg
     4921 ggcgattttg tcagcgctcc agccggccat tttcagcgga aaggcgatgt tttcgcgcac
     4981 ggtcatgtgc ggaaacagcg catagctctg gaataccgta tgcaccgggc gcttttcggg
     5041 cggcacattg gacacatcct tgccatccag caggatttcg ccgctgtcgg gctgttcaaa
     5101 gccggccagc atgcgcaaga gcgtggtttt gccgcagccg gacggcccca gcagggtgaa
     5161 gaactcgcca gcgttgaccg acaggctgac gtggttgacc gcggtgaagt ccccgaaccg
     5221 cttgactaca ttacgaattt caagaagcgc catggtgttc ccgtccgacc ggcggccgga
     5281 cgccgtgaga agaattcaat ccgtcgcgcc tggcccaagg ccgctgcgcg tcccgtaaca
     5341 tgacatagtg ccggaaatcc ggccccccgt catgctgggg gtgtggaact gggttccgta
     5401 gcgtggcttg tgggggtgaa gcaaggggtg ggccacggcg atgaggtggc attgttcagc
     5461 gccacttggc gtcgctgaca aaactcagcg cagcgattct ggctaggtgt gcggccgtag
     5521 gcagtgcaag ccgtacggca aggccgcaca acgacgccag gagggtgagt ggcgctcagg
     5581 cccggccggg cgtcgcacgc cgatcaagct gggcgctctc gggtgcgaca tcggcgcaag
     5641 atagttgcgc cgacaggggg ttggcatcaa agcagggccg gcgtgagcgc gccggctcag
     5701 tcggcctggg gctggctcgg ggcgatcagt tcgcccggac gggcggcggc tgcggcggcc
     5761 aggcggtctt gctcggcgcg ctggcggagg gtgtccagat agtccatcac cgcgtaggtc
     5821 agctcgcgcg tgccttcgcc ggtcagcgcc gaaatcacaa agtggcgcgg cgccatcggg
     5881 tcgaagtcgg cgtaggggtc ggggttttcc tgccagccat agtcggcaag gaactgctgc
     5941 acgcgggcgg tgcgctcgtc gtcgtccacc atgtccagct tgttcagcac cagccagcgc
     6001 ggtttgttgt acagcgattc gtcgtatttg cgcagctctt cgacaatcgc gcgcgcctcg
     6061 gccaccgggt cggtgtccgg gtcaaacggg gccaggtcca caatgtgcag cagcaggccg
     6121 gtgcgctgca aatgcttgag aaagcgatgg cccaggccag cgccatccgc cgcgccttcg
     6181 atcaggccgg ggatgtcggc aatcacaaag ctgcggtttt cgtcgatgcg caccacgccc
     6241 agattggggt gcagggtggt gaacgggtaa tccgccacct tgggccgcgc ggcggacacc
     6301 gagcggataa aggtggattt gccggcgttg ggcatgccca acaggccgat gtcggccagc
     6361 accttgagct ccagcttcag ctcgcgctgc tcgcccggct cgccgtgggt aaactggcgc
     6421 ggcgcgcggt tgaccgagct tttaaagtgg atattgccca gcccgccctt gccgccacgg
     6481 gcgatgatga cttccatgcc gtcggtggtc aggtcggcca ccacttcgcc ggtcagcgaa
     6541 tcggtaatca gcgtgcccac cggcatgcgc aggataatat cgtccgcgcc cttgccatag
     6601 cagtcggcgc cccggccgtt ttcgccgttg cgggcgatgt attttttcac aaagcggtag
     6661 tccaccaggg tgttgatgtc tttgtccgcc acggcaatca cattgccgcc gcgcccgccg
     6721 tcaccaccat ccgggccgcc gcgcgggacg aatttttccc ggcgcatgct ggccgagcca
     6781 ttgccgccct tgccgccgca cacttcgatg cgcgcttcgt cgatgaattt catgtcactc
     6841 tccttgaacg cagccaagtt tggccgcgct gtgcgcgggg caacggtgag ccatcagccg
     6901 ccgcgccggg caggggctca tggttcacgg ctgcgccgca aacaacaaaa agagccctat
     6961 cgtgggatag ggctctgatg acgctaaaac tgcgtgtaaa ttacgcggct tcggcttcgc
     7021 cagtgtacgg cagcacagtc acggtcttgc gcttggcggc gcctttgacc tcaaacttga
     7081 catagccgtc aaccttggcg aacagggtgt gatccttgcc catgccaacg ttgtcgccag
     7141 cgtggaagcg agtgccacgt tgacggatga tgatgctgcc agcgggaatc agttcgccgc
     7201 cataagcctt ggtgcccagg cgcttggact cagagtcacg accgttgcgc gagctaccgc
     7261 ctgccttttt gtgtgccatg agagttctcc cggattactt cgaaatcgcg tcgatgcgaa
     7321 tttcggtgta gttctgacga tggccctggt gcttttggta gtgcttacga cgacgcatct
     7381 tgaagatgcg gatcttttcg ccacgaccat gcgacacaac ggtcgcagtc acggttgcgc
     7441 cggccaccag cggggtaccc actgcaacct gatcgccatc agcaaccatc agtacctgat
     7501 ccaggacgat ttggctgtcg atgtctgctg gtatctgttc tactttaagt ttttcgccga
     7561 tggtcacgcg atactgcttg ccaccggttt ttacgaccgc atacattcgt taagctccta
     7621 aaaatacgtt tcctcgcgag cctttcgcgg gaagcgagtg actatacgca agttgccggg
     7681 cggcggcaag attttttgcg cgcaacccgc ttgattggca aggcgcagga ttttttgcct
     7741 gggccaccgc atggcgtcgc agcggcaaat ctccacagac cctcccacgc caagcttatg
     7801 ccccacctgc ctttgccatt cggtttccgg caggcacaat gacgggctca ctcaactgtc
     7861 tgtccgctcg cgcccgccat gcctccgatc cagcgtcctt ccgtttgtcc ccatgattgc
     7921 cccagtgtct gcgccctgtc agtagaggtg ggcgacgatg gtcgcattgg ccgcgtgcgc
     7981 ggtgcagcac agccttacac cgacggggtg atctgcgcca aggtggcgcg ctacgccgag
     8041 cgcgtgcacc accccgagcg gctaacccag ccgctgttgc gggtgggcga caagggcagc
     8101 ggccagttca ccccgatcag ctgggacgcc gcactggatc tgttggccga gcgcatcgtc
     8161 agcgccaccg cccgccacgg cgcgcaaagc atctggccct accactacgc cggcaccatg
     8221 ggcgtggtgc agcgcgccgc catccgccgg ctgggccatc tggccggctg gtcgcgccag
     8281 cgggaaacct tctgcgtggc cttgtccgac gccggctggc tggccggcgt cggcgccaag
     8341 cgcgggctgg acccgcgcga ggtgatggag tccgagctga ttgtggtgtg gggcggcaac
     8401 ccggtgcaca cccaggtgaa tttcatgcac tggctgcaaa aggcccggcg cgggcgcgat
     8461 gtaccgctgg tggtggtgga cccctaccgc accgccactg ccgccaaagc cgatgtgcac
     8521 ttggctttgc gccccggcac cgatggcgcg ctggcctgtg cagtgatgca tgtgctgctg
     8581 gccgaaggcc tggccgaccg ggattatttg gccaaacaca ccgatttctc ccccgaggtg
     8641 gaagcccatc tggccagccg cacgccggcc tgggcggccg agatcaccgg cttgtcggtc
     8701 gagcagattg tgcagtttgc ccggctgtat ggcggcgcgc ggcgcagctt tttgcgtgtg
     8761 ggctatgggt tttcccgcca gcgcaatggc gcggcggcca tgcacgcggt cagctgtctg
     8821 ccggcggtca ccggcgcctg gcaatggccg ggcggcggcg cgctgtacag caacggcggc
     8881 ctgtacgggc tgaacacccg cgcactgtac ggcctggacg ccgtcacccc ggacacccgc
     8941 gtgctggaca tgggccggct gggcgcggtg ctggctggtg aacggcgtga cctgggcgat
     9001 ggcccgccgg tcagcgtgtt gctggtgcaa aacaccaatc cggcagtggt ggcgccggac
     9061 agcctggcgg tgcggcgcgg cctgctgcgt gacgacctgt ttgtctgcgt gcacgaacag
     9121 ttcatgaccg acaccgcgcg cctggccgat ctggtgctgc cggccaccac ctttctggag
     9181 cacgacgacc tgtatcaggc ctctggccac acctttttgc aggcggcgcg cgcgctgatt
     9241 gcgccgctgg gcgaatgccg cgccaaccat gacttcatca gccagctggc cgcgcggctg
     9301 ggggtgtcgc atccggcttt tcagcaagac gcctgggcgc tggtggacca ggtgttgcgc
     9361 gagagcggcc agcccgacgc cgacagcctg tacgccgccg gctggcatga ctgcacgcca
     9421 ggctttgccc gcgcgcattt tctggaaggc tttggccatg ccgacgggcg ctttcgcttt
     9481 gccccggatt gggcggcgcg cggcgaaggc cacgccagca tgccgcgcta ccccgaccat
     9541 caggcggtga tcgacgccgc cagcgccgag cggccgttcc ggctggtggc cgcgccggcg
     9601 cggcactttt tgaactccag ctttaccgaa acccccagct cacgccaact ggaaggccgc
     9661 ccgaccgcgc aactgcaccc cgacgccgcc gcgcgcctgg gcattgccga cggcgagctg
     9721 atccgcctgg gcaacgccca ggccagcgtg ctgctgcacg cccgcaccgt tgccggcgtg
     9781 cagcccgaca ccgtggtggt ggaaagccag tggccaaacg acgcgtttat cgaaggggta
     9841 ggcatcaatg cgctggtcag cgctgaaccg ggctttccgg cggcgggggt ggcgtatcac
     9901 gatacggcgg tgtgggtgca gcgggtggcg ggcgaataaa agccgttcaa aaaccctgcc
     9961 tggcgtgctg gtgcgcatcc tgcccgtatt tgcgccgctg cggctgaatg ccgccatgcg
    10021 tggcttgctg gagcgagtat tgcccagcgc ggggtttctg agtttcctgc tgtatatcgt
    10081 ctggaccgaa tggcaggcgg cgccgtggcc agcggccatt gggctgacct tggcggcagg
    10141 gctgacgctg ttcacccgca ccgggctgat tggcgctacc gccttaggct cggtggtgta
    10201 tttcgcctgt ggcttggtgt ggcgctagcg caggcgcgcc aggactgccc ggggtcgttc
    10261 agtcaactcg gcatggcgat tctgggcaag cgcaccgcca cgcacctcgt ctatcaggcg
    10321 gtagtgccac acgaccaagc gcatttttct tggtgttaca accgccatgc gcagtgagct
    10381 ggctgctatg ctagcgcctt gctgacaacc gccctgttcc tcccatgacc gctgccgcac
    10441 cccgcccctg gcccgcgccc atccgcgcgc tgggccatgc gcattttcgc cgttatttcg
    10501 ctggccaggc ggtgtccatc cttggttcgt ggattcagca ggtggcgatg gcctggctgg
    10561 tgtatcggct caccggctcg gcggccttgc tgggggtgac tgcgtttgcc gcgctgattc
    10621 cgcagctgct ggtggggccg tttgccgggg cgtgggtgga caagcacgac aagcgccgcg
    10681 cgctgttgtg ggtgcaggcc ttgctcaccg tgcaggcgct ggcgctggcg gcgttgacct
    10741 ggctgggctg ggtcagcccg tggctgctgg tggcgatgtc tggcctgctg ggcgtgctca
    10801 atagctttga cacgccattg cggcaatcgc tgattggcag ttttgttggc cggcgtgaag
    10861 atttgcccaa tgcgctggcg ctgaacgcca tgctgttcaa tagcggccgc tttatcggcc
    10921 cgccgattgc cggtgcgctg ctggcggcct tgtccgaagc ggcgtgtttt gcgctgaacg
    10981 cgctgacttt tctggcgctg atcatcgccg tggccagcat ccgcatgccg gcgccgccca
    11041 aggcgcaggg ctcgatgggc gatgtgtttc gtgaaggggt cagctacgcc tggcacacct
    11101 ggctggtgcg gctgctgatc tttaccctga ttgcgctcaa cctcaccgcc tcggcctacg
    11161 ccgtgctgct gccggtgttt gcccgcgatg tgtttgccgg cgatgcgcgc acgctgggct
    11221 ggctgtgggg cgcggcgggc tgcggggcgt ttgtgtccac gctgtatctg gccacccgcc
    11281 aatccatgcc cggcctggtg cgcagcatcg tctggggcgc ggcgctcagc gcgctggcgc
    11341 tgttggtgtt tgggctcacc gcctggctgc cgctggcgct ggcggcgatg gcggcgctgg
    11401 gctttggcat ttcggtgagc aatgtcggca tcaatatgct gctgcaatcc accgcccccg
    11461 accggctgcg cggccgggtg gtgtcgtttt tcacctcggc gcgctttggc tttgacgccc
    11521 tgggcggctt gctggccggc ctgctggcca ccgcgctggg cgccgggtgg acgctggcgc
    11581 tggaaggcgt gctgctgctg ggctttgtgc tgttcttgct cagccgccgc cgggctttgc
    11641 gccaggcgat tgaccggcac gatccgacgg cgcatt
//
LOCUS       DAOPYX010000055        11597 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_6329_length_11597_cov_7.953821, whole genome shotgun sequence.
ACCESSION   DAOPYX010000055 DAOPYX010000000
VERSION     DAOPYX010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 11597)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11597)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11597
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6329_length_11597_cov_7.953821"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            370..1407
                     /locus_tag="PKN55_04420"
     CDS             370..1407
                     /locus_tag="PKN55_04420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011135049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HNX70519.1"
                     /translation="MIVRPARTTDLPALEHMAAASAAGVTGLTPHREYLFERIQQSIA
                     ALDSDVAWQSEESYTFMLEVDGQVVGTAGLIASAGFTQPFYSYRNETLIHASAALGVN
                     NKIHALTLCNDLTGVTQLCGFYLDPAFLDSAAADMLSRARLLFIASHPQRFSERLFDE
                     MQGHTDAEGRYPFWEAIGKPFFKMEVEEVDRRIALGNKTFIAELMPTYPIYVPLLPDA
                     AQQAMGQIHPDAEVPFAILMDEGFEADDYIDAIDGAPTLTAKTHWVRSIMHSRIAQLH
                     IIDNRPAGTETYLICNQRTSQFRAVLADVLWHGDEVWVSPDTAQALACEPGDSVRLTP
                     FTPVVGQGEQA"
     gene            1404..2453
                     /gene="astA"
                     /locus_tag="PKN55_04425"
     CDS             1404..2453
                     /gene="astA"
                     /locus_tag="PKN55_04425"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008954501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008791 - arginine N-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HNX70520.1"
                     /translation="MMLIRMARPDDLDALVALARLADIGVTSLQADPAQLAERIERSQ
                     RTLRNELPRANQGYLFVLECSDTGQLVGTCSIESALGLHSPWYNYRVGKVIHASPSLN
                     IYSSAETLFLSNDHTGSTEVCALFLHPDWRREHLGAMLSKCRFLFMAQHPERFANKVI
                     AEMRGVADEQGRSPFWEALGRHFFTVEFAQADHLTGVGDKSVVAELMPKYPVYVDLLP
                     AEARAVIGQVHEKTQPALAMLQEEGFRYQGYVDIFDAGPTVEAYLHEIRAVRHSRLWT
                     VGEEKAAPSAHAQPWLLASTGLSDFRMTVKLLDAPHGVLPLDDDTAQMLGVREGDVLR
                     AVPLHPPQDLSQVLP"
     gene            3057..3986
                     /locus_tag="PKN55_04430"
     CDS             3057..3986
                     /locus_tag="PKN55_04430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017510038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HNX70521.1"
                     /translation="MDIFLQQILNGLVLGSIYALIALGYTMVYGIMGLINFAHGEVVM
                     FGAMVTISIIGMLTGMGLSLPGPVVVLLALMLAIPVCMLMGFAIERIAYRPLRNAPRL
                     APLITAIGLSIVLQNVAILIWGRNYIPFPPLLPHDPINVLGASITDLQVFIVISAIAI
                     MAGLLFMVEKTKLGRAMRATSQNPAVAGLMGVNVNTIISATFVLGAGLGAVAGVMVAA
                     NYDQAHSHMGFLIGLKAFTAAVLGGIGNLGGAVVGGILLGVIESLGAGYIGDLTGGFL
                     GSHYQDIFAFAVLILVLVFRPSGLLGERVAERA"
     gene            4006..5115
                     /locus_tag="PKN55_04435"
     CDS             4006..5115
                     /locus_tag="PKN55_04435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012697383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNX70522.1"
                     /translation="MPQNQSKRMLAFALSGIVLAVLPFIVGSTLGNSWVRTLDFALLY
                     IMLALGLNIVVGFAGLLDLGYIAFYAVGAYTFALLNSPHLSIFMPWWFILPLGAILAA
                     FFGIMLGTPVLRLRGDYLAIVTLGFGEIIRIFMNNLNAPVNITNGPQGINMIKPIEVA
                     GFSLGKPVELFGTTYNSVYIYYYLFLLLAVLTVIICHRLQHSRIGRAWVALREDDVAA
                     NAMGLNIRNIKLLAFAMGATFGGVAGGLFASFQGFVSPESFGLMESIMVLAMVVLGGM
                     GNISGVILGAVLLTVTPELLRDIIGPLQMKAFGRMIVDPENARMLMFGLALIVVMLVR
                     PEGLWPSKRRKAEFHDALDDGIPAADSQAADGLKG"
     gene            5120..5890
                     /locus_tag="PKN55_04440"
     CDS             5120..5890
                     /locus_tag="PKN55_04440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008954506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNX70523.1"
                     /translation="MADALLKIEGIHKRFGGLHALNDVGLTINRGEIYGLIGPNGAGK
                     TTLFNVLTGLYQPDEGKFTFNGQDLFRQKPHVVVKAGIARTFQNIRLFGEMTALENVM
                     VGRHVRTKAGALGAILRDRATMAEEKAIRDRARELLNYVGIGNVANERARNLSYGHQR
                     RLEIARALATDPTLLALDEPAAGMNPRETEDLQALMEKVRSSGVTLLLIEHDVKLMMT
                     LCDRIAVLDYGKKIAEGVPQEVKSNPRVIEAYLGAAHA"
     gene            5887..6597
                     /locus_tag="PKN55_04445"
     CDS             5887..6597
                     /locus_tag="PKN55_04445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011135058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNX70524.1"
                     /translation="MSLLEVKNLKVAYGGIQAVKGIDLHIDQGELVTLIGANGAGKTT
                     TLRTLVGMVKPAEGTIQYDGKPTGKTPVFDFVRKGLVMVPEGRGVFGRLTVEENLRMG
                     AYYRNDESAIRSEIDYVYELFPRLKERMTQLAGTLSGGEQQMVAMGRAMLSKPKLLLL
                     DEPSMGLAPIIVQKIFEIVQMISKQGVTILLVEQNAKLALEISDRGYVMESGRITMSG
                     AASELLSSDAVQKAYLGE"
     gene            6608..7378
                     /locus_tag="PKN55_04450"
     CDS             6608..7378
                     /locus_tag="PKN55_04450"
                     /inference="COORDINATES: protein motif:HMM:NF014724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HNX70525.1"
                     /translation="MLIERPELWLSALVGAFVLPPGVWLWLLALAAWQRRRRPWLARL
                     CVGGTMLTLYALSTPWLAARLQQSVEIATPIRAADLQQVDAIVVLGGGRREAAADQGG
                     MDTISDGQLVRLRHAAQLARQSGKPLLASGGSPEGGPAEAELMALSLAQDFATPVRWQ
                     ETRSVNTAENARFSAEILHAAGVRRIALVTHSWHMPRALRSFAATGLQVIPAPTGQSR
                     PTLRLPYSVLPSAQALWHSSQALREGVGLLAYALRGVF"
     gene            complement(7463..8143)
                     /locus_tag="PKN55_04455"
     CDS             complement(7463..8143)
                     /locus_tag="PKN55_04455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nuclease-related domain-containing protein"
                     /protein_id="HNX70526.1"
                     /translation="MIIKPPDSLQARLAAQRATLPATDPASEARLRGLHQHLRGLQHS
                     ANDLDAQLADGTRWALLHDLHLRIDGRSLWIDHLLINRQLDILLLASPHYGDSLSVDE
                     QGEFFREVRGRRLGFASPLNRLHRQLALLRTWSRQLAWPYRQGGPLTPSWLFAVLLAP
                     HADSEAPAAFAELLVRPHQLNDWLAIHTRHPERSTLDTLAHSVSVDTLRGFAQQWRDR
                     HIGPEQAG"
     gene            complement(8162..8533)
                     /locus_tag="PKN55_04460"
     CDS             complement(8162..8533)
                     /locus_tag="PKN55_04460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX70527.1"
                     /translation="MSRFNNHHHSHQHSPGRQPGYSGAHAGNKDVPPHCQGKLRSLVT
                     NQTYPALCYDVRITGMQVCTPYVPQQGERIELTVYPPDIGGRPSEPFTTEVEAVCCLE
                     VQQGQLYQLTVNIVTSSVRRR"
     gene            complement(8520..9077)
                     /locus_tag="PKN55_04465"
     CDS             complement(8520..9077)
                     /locus_tag="PKN55_04465"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012697732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HNX70528.1"
                     /translation="MALTEHTLDSEQLLEGGFLRVYRDRVRLPDGSEARREYIAHPGA
                     VAILPLTEAGELVLVRQYRYPVRQTFLEIPAGKIDAGEAWLATAQRELLEETGYTARR
                     WHALPVAHPCIGYSDEKIAYFVAEGLIAGDSQPDEGEFVEVEHLPLTEVIARAQRGEL
                     SDSKTLTGLFWLQAWQSGMLRSEPL"
     gene            complement(9064..9624)
                     /locus_tag="PKN55_04470"
     CDS             complement(9064..9624)
                     /locus_tag="PKN55_04470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012697733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2062 domain-containing protein"
                     /protein_id="HNX70529.1"
                     /translation="MPRKWFKRYLPDPATLAANRWLAWMGPRLQDPQLWTLHRRNVAR
                     AVAVGLFAGLMPGPTQMLTAGALAWCWRINLPVALATTLYTNPLTIVPLYWLAYEYGC
                     LLTGQQGGLPALPPEWATQGFGVWLGQMVDWAQTLGWPLLLGVPAQGLTLGLAGHGLV
                     MLAWRWHTVHAWRHRRHARKTSHGSD"
     gene            9856..>11597
                     /locus_tag="PKN55_04475"
     CDS             9856..>11597
                     /locus_tag="PKN55_04475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008954351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HNX70530.1"
                     /translation="MPAYKAPLRDFDFVLNEVIQVQNVISTLPGYEEATQDVFASYLD
                     AAAKFCENELAPINRAADEEGCSFNPADHSVTTPAGFKEAYQQYCELGFTGLDCDPAY
                     GGQGMPKVLAFPMMEMQCSANVAWSMYPGLSHGAYAAIHAHGTDEQKATYLPKLVDGS
                     WTGTMCLTEPHCGTDLGLLKTKAEPNGDGSYSITGTKIFISAGEHDMSDNIIHLVLAR
                     LPDSPKDVKGISLFIVPKFHADGSRNPVFCGALEHKMGIKANATAVINLDGAKGYLIG
                     ERDKGLACMFTMMNAARLGCGMQGLGLGEASFQGALSYARERLQMRALTGPKAPEKAA
                     DPIIVHPDVRRMLLTQKAYTEAGRAMTAWLALQLDIEEKSPDADARSQAADLVALLTP
                     VAKAFMTDNGYTAANMGMQVFGGHGFIREWGMEQLVRDCRISQIYEGTNGIQAIDLLG
                     RKVLMDQGQKLRKFTKIVHKYCEANGGDSRLKDMAEAVSKLLKDVGDVTMHVGMQSMM
                     NRDEAGAAATDYLRLIGHLTYGFFWLWISKTALDKLDAGTGEKAFYEAKLTTARFYFA
                     KLFPEVHSLLVTLKA"
BASE COUNT         2206 a         3606 c         3548 g         2237 t
ORIGIN      
        1 gatgatagtg cggattaccc gtgtgaaagt aggtcaacgc caggctccta ttcaaacccc
       61 ctgtagcaac gctacagggg gttttgtgtt ttatgcattc acttctgagc gcatctcttg
      121 tgtgaatttc tgctgatttt tggcaaaaaa tcagagaaaa ccacgtcttc gcaatctgta
      181 cgcctgtgtc gcaaacccga aatgcctgct tgtcgaattt gattaaaatc agataaagcc
      241 gttgtccggc cagaagacgc cccattgtca tctgcacccg gcaaaacggc atggattcgc
      301 ccgaccgcca ccccccggcg gcctgggctg gcgtgtgttg cgcgaacagg ctagggagtg
      361 agtggcatga tgattgttcg accggcgcga acaaccgatt taccggcact ggaacacatg
      421 gctgccgcca gtgcggccgg cgtgaccgga ttaaccccac atcgggaata tctgttcgag
      481 cgcatccagc aatcgattgc cgccctggac agtgatgtgg cctggcaaag cgaagaaagc
      541 tacaccttca tgctggaagt tgatggccag gtggtgggca cggccgggct gattgcctcg
      601 gcggggttca cccagccgtt ttacagctac cgcaatgaaa ccctgattca cgcctcggcg
      661 gcgctggggg tgaacaacaa gattcatgcg ctcactctgt gcaatgacct gaccggcgtc
      721 actcagctgt gtgggtttta tctggatccg gcgtttctgg attctgccgc cgccgatatg
      781 ctgtcgcgcg cgcgcctgct gtttattgcc tcgcatcccc agcgcttttc cgagcgcctg
      841 tttgacgaaa tgcagggcca caccgacgcc gaagggcgtt accctttctg ggaagccatc
      901 ggcaagccgt ttttcaagat ggaagtcgaa gaagttgacc gccgcattgc cctgggcaac
      961 aagaccttca tcgccgagct gatgcccacc tacccgattt acgtgccgct gctgcccgat
     1021 gccgcccagc aggcgatggg ccagattcac ccggatgcgg aagtcccgtt tgccatcctg
     1081 atggatgaag gcttcgaggc ggatgattac atcgacgcca tcgacggcgc gccgacgctg
     1141 accgccaaaa cccattgggt gcgctcgatc atgcatagcc gcatcgctca gctgcacatc
     1201 atcgacaacc gtccggccgg caccgaaacc tatttgattt gtaaccagcg caccagccaa
     1261 ttccgcgccg tgctggccga tgtgctgtgg catggcgacg aagtctgggt gtcgccggat
     1321 accgcccagg cgctggcctg tgagccgggc gacagcgtgc gccttacccc gttcaccccg
     1381 gttgtcgggc aaggagagca agcatgatgc tgatccgcat ggcgcgtccg gacgatctgg
     1441 acgccctggt ggcgctggcc cgactggctg atattggcgt cacctcattg caggccgacc
     1501 ccgcccagct ggccgagcgc atcgagcgca gccagcgcac cttgcgcaac gagctgccgc
     1561 gcgccaatca aggctatctg tttgtgctgg aatgcagcga caccggccaa ttggtcggca
     1621 catgcagcat tgagtcggcg ctgggcctgc attcgccctg gtacaactac cgggtgggca
     1681 aggtgatcca cgcctcgccg tcgctgaata tctattccag cgcagaaacc ctgtttctgt
     1741 ccaatgacca caccggcagc accgaagtgt gcgcgctgtt cctgcacccg gactggcggc
     1801 gtgagcactt gggcgccatg ttgtccaagt gtcgttttct gttcatggcg caacaccccg
     1861 agcgctttgc caataaagtc atcgccgaaa tgcgcggcgt ggccgacgaa caaggccgct
     1921 cgccgttctg ggaggcgctg ggccggcatt tcttcaccgt ggagtttgcc caggccgatc
     1981 atctgaccgg ggtgggcgac aagtctgtgg tggccgagct gatgcccaag tatccggtgt
     2041 atgtcgatct gctgccggcc gaggcgcgcg ctgtgattgg ccaggtgcac gaaaaaaccc
     2101 agccggcgct ggccatgctg caagaagaag gcttccgcta ccagggctac gtcgatattt
     2161 ttgacgccgg cccgaccgtg gaggcctatc tgcacgaaat tcgtgcagtg cggcacagcc
     2221 ggctgtggac ggtgggcgaa gaaaaggccg cgccatcggc gcacgcccaa ccgtggctgc
     2281 tggccagcac cggattgagc gattttcgca tgacggtgaa gctgctggat gcgccgcatg
     2341 gcgtgctgcc gctggatgat gataccgccc agatgctggg tgtgcgcgag ggcgatgtgc
     2401 tgcgggcagt gcctttgcat ccgccgcagg atttaagcca ggttttaccc tgaaaatacc
     2461 ctgtattaag agaaatatct tgccacgcat tcaattgtgc gtggctgttt gagcatgaac
     2521 tgcttgcctt cgctgatttt gtggtggaaa gtgttggcaa gctggcgcgt ttgctgcgct
     2581 gattgtttaa aaacgaacat tcctggcact tttcctgctt atatctgttc gttctgtatc
     2641 aatgtatttg cgacaagtga gttttacccg gtgtttttca atgtgcgctg cacaattcag
     2701 gtgcgttgca caatttgagt gctaaataag ctttcaggag cttgtcatgc tgaccggtaa
     2761 tgcagatcaa gccatctcgg tttgcattgc aacataacga ttaccgtatt tatacgtatc
     2821 gtttttattt tttgttgtaa aatcattgta ttgcagaatg gcttcccaat ggggcaaacc
     2881 caatgtggcg gcaggtaaag cggttcagat tatgattgac agcgtgtaat cagctagaca
     2941 taatccgacc gctcgcgcgc tgacggtgtc aaaaacctgt tggcgcgtgc taataaaacc
     3001 aacgtgccgt cggttcacca gaccgactga ggcggctctc aaggagattt cccacagtgg
     3061 atatttttct tcaacaaatc ctgaacgggc tggtgctcgg cagtatttat gcgctgattg
     3121 cgctggggta taccatggtg tatggcatca tgggtctgat caactttgcc catggcgagg
     3181 tggtgatgtt tggcgccatg gtcaccatct ccatcatcgg catgctgacc ggcatgggct
     3241 tgtctttgcc cggcccggtc gtggtgctgc tggccttgat gctggccatc ccggtgtgca
     3301 tgctgatggg cttcgccatc gagcgcattg cttaccgccc gctgcgcaat gcgccgcgtc
     3361 tggccccgct gatcaccgcc attggcctgt ccattgtgtt gcagaacgtc gccatcctga
     3421 tctggggccg caactacatt cccttcccgc cgctgttgcc gcacgacccg atcaatgtcc
     3481 tgggcgccag catcactgat ctgcaagtgt tcatcgtgat ctccgccatc gccatcatgg
     3541 ccggcctgtt gttcatggta gaaaaaacca agctgggccg cgccatgcgc gccaccagcc
     3601 aaaacccggc agtggctggc ctgatgggcg tgaacgtcaa caccatcatc tccgccacct
     3661 ttgtgctggg cgccggtctg ggcgcggtgg ccggcgtcat ggtggccgcc aactacgacc
     3721 aggctcactc gcacatgggc tttctgattg gcctgaaggc ctttaccgcc gctgtgctgg
     3781 gcggcattgg caacctgggc ggcgccgtgg tcggcggcat cctgctgggg gtgatcgagt
     3841 cgctgggcgc tggctatatc ggcgacctca ccggcggctt cctgggcagc cactatcaag
     3901 acattttcgc cttcgccgtg ctgatcctgg tgctggtgtt ccgtccgtcc ggcctgctgg
     3961 gcgaacgcgt ggccgaacgc gcctgattcg gggagaacta cacacatgcc gcaaaaccaa
     4021 tccaaacgga tgcttgcctt tgccctgtcg ggcatcgtgc tggccgtgct gccctttatt
     4081 gtcggcagca ccctggggaa ctcctgggtt cgcaccctgg acttcgcatt gctgtacatc
     4141 atgctggcgc tgggcctgaa catcgtggtc ggcttcgctg gcctgctgga cctgggctat
     4201 atcgccttct acgccgtggg cgcctacacc tttgccctgc tgaactcgcc gcacctgagc
     4261 atcttcatgc cgtggtggtt cattctgccg ctgggcgcca ttctggcggc cttcttcggc
     4321 atcatgctgg gcacgccggt gttgcgcctg cgcggtgact atctggccat cgtcaccctg
     4381 ggctttggtg aaatcatccg cattttcatg aacaacctga atgcgccggt gaacatcacc
     4441 aacggcccgc aaggcatcaa tatgatcaag ccgatcgaag tggccggctt ctcgctgggc
     4501 aagccggttg agctgttcgg cacgacctac aacagcgtgt acatctatta ctacctgttc
     4561 ctgctgctgg ccgtgctgac cgtgatcatc tgccatcgcc tgcaacactc ccgcattggc
     4621 cgcgcctggg tggccttgcg tgaagacgac gtggccgcca acgccatggg cctgaacatc
     4681 cgcaatatca aactgctggc ctttgccatg ggcgccacct ttggcggcgt ggccggcggc
     4741 ctgttcgcca gcttccaggg ctttgtctcg ccagaatcct ttggcctgat ggaatccatc
     4801 atggtgctgg ccatggtggt gctgggcggc atgggcaata tctccggcgt gattctgggc
     4861 gccgtgctgt tgaccgtcac cccggaactg ctgcgcgaca tcattggccc gctgcaaatg
     4921 aaagcctttg gccgcatgat cgtggacccg gaaaacgccc gcatgctgat gtttggcctg
     4981 gcgctgattg tggtgatgct ggtgcgcccg gaaggcctgt ggccgtccaa gcgccgcaag
     5041 gcagaattcc acgatgcgct ggacgacggc attcctgccg ccgactccca ggctgccgac
     5101 ggactgaaag ggtaatgaca tggccgacgc actgctgaaa atcgaaggta tccacaaacg
     5161 ctttggcggc ctgcacgcgc tcaacgacgt aggcctgacc atcaatcgcg gcgaaatcta
     5221 cggcctgatc ggcccgaatg gcgccggcaa gaccacgctg ttcaacgtgc tgaccgggct
     5281 gtatcagccg gacgaaggca agttcacctt caatggccag gacttgttcc gccagaagcc
     5341 gcacgtggtg gtgaaggccg gtattgcccg caccttccag aacattcgcc tgtttggcga
     5401 aatgaccgcg ctggaaaatg tcatggtcgg ccgtcatgtg cgcaccaagg ccggcgccct
     5461 gggcgccatc ctgcgcgacc gcgccaccat ggccgaagaa aaagccatcc gtgaccgcgc
     5521 ccgtgagctg ctcaactatg tgggcatcgg caatgtcgcc aacgaacgcg cccgcaatct
     5581 gtcctacggc caccagcgcc gcctggaaat cgcccgcgcg ctggccaccg acccgaccct
     5641 gctggcgctg gacgaaccgg ccgccggcat gaacccgcgt gaaaccgaag acctgcaagc
     5701 cctgatggaa aaagtccgca gcagcggcgt gaccctgctg ctgatcgaac acgacgtcaa
     5761 gctgatgatg accttgtgcg accgcatcgc cgtgctggat tacggcaaga aaatcgccga
     5821 aggcgttccg caagaagtca agagcaaccc ccgagtcatc gaggcctacc tgggagcggc
     5881 acacgcatga gcctgttaga agtcaaaaac ctcaaagtgg cctacggtgg cattcaggct
     5941 gtcaagggca tcgacctgca cattgaccaa ggcgagctgg tgaccttgat cggcgccaac
     6001 ggcgccggca agaccaccac cctgcgcacc ctggtgggca tggtcaaacc ggccgaaggc
     6061 accattcagt acgatggcaa gccgaccggc aaaaccccgg tgttcgactt tgtccgcaag
     6121 ggcctggtga tggtgccgga aggccgtggc gtgtttggcc ggctgaccgt cgaagaaaac
     6181 ctgcgcatgg gcgcttacta tcgcaacgac gaaagcgcca tccgcagcga aatcgactac
     6241 gtctacgagc tgttcccgcg cctgaaagag cgcatgaccc agctggccgg caccctgtcc
     6301 ggcggcgagc agcaaatggt ggccatgggc cgcgccatgc tgtccaagcc caagctgctg
     6361 ctgctggatg agccgtcgat gggtctggcg ccgatcatcg tgcaaaagat cttcgaaatc
     6421 gtgcagatga tttccaagca aggcgtgacc attttgctgg tcgaacaaaa cgccaagctg
     6481 gcgctggaaa tctccgaccg tggctatgtg atggaatccg gacgcatcac catgtccggc
     6541 gccgccagcg aactgctgag cagcgacgct gtgcaaaaag cctacctcgg cgaataaggc
     6601 gcagcccgtg ctgatcgaac gccccgaact gtggctgtcc gccctggtcg gggcgtttgt
     6661 tttgccgcct ggcgtctggc tgtggctgtt ggcgctggct gcctggcagc gccgccgccg
     6721 gccctggctg gcgcggctgt gcgtgggcgg caccatgctg acgctatacg cgctgtccac
     6781 cccctggctg gcggcgcggc tgcaacaatc ggtggaaatc gctacaccca ttcgcgccgc
     6841 agacttgcag caggtggacg ccatcgtggt gctgggtggc ggccggcgtg aagccgccgc
     6901 cgaccagggc ggcatggaca ccatcagcga cggccaactg gtgcgtctgc gccacgctgc
     6961 ccagctggcg cgccaatccg gcaaaccgct gctggcctcc ggcggcagcc cggaaggcgg
     7021 cccggccgag gccgagctga tggccttgtc gctggcgcag gactttgcca cgccagtgcg
     7081 ctggcaggaa acccgctcgg tgaataccgc cgaaaacgcc cgtttcagcg ccgagatcct
     7141 gcacgccgcc ggcgtgcgcc gcattgccct ggtcacccac agttggcata tgccgcgcgc
     7201 gctgcgcagt tttgccgcca ccggcttgca agtcatcccc gcccccaccg gccaaagccg
     7261 gcccaccctg cgtctgccgt atagcgtgct gcccagcgcg caagcgctgt ggcacagcag
     7321 ccaggcgctg cgcgaaggcg tgggcctgct ggcttatgcg ctgcgtgggg tgttctgagc
     7381 gtaagcgcgg cgggtgctgg caacaaaaaa caacggcggt cgatgattcg accgccgttg
     7441 ttttttgatg aggcactgtt tttcagcccg cctgctccgg cccaatatgc ctgtcacgcc
     7501 actgctgggc aaagccgcgc aaggtgtcca ccgacacact gtgcgccagg gtatccagcg
     7561 tgctgcgctc gggatggcgg gtgtggatgg ccagccagtc gttcagctgg tgtgggcgca
     7621 ccagcagctc ggcaaatgcg gcgggcgctt cgctgtcggc atggggcgcc agcagcacgg
     7681 caaacagcca gctgggtgtg agcggcccac cctgacggta tggccaggcc agttgccgcg
     7741 accaggtgcg cagcagcgcc agctgacggt gcaggcggtt cagcggcgag gcaaagccca
     7801 ggcggcggcc gcgcacttca cgaaaaaact cgccctgctc gtccacgctc aggctgtcgc
     7861 cataatgcgg gctggccagg agcagaatgt ccagctggcg attgatcagc aaatggtcaa
     7921 tccacagcga gcgcccgtcg atacgcagat gcaggtcgtg cagcagcgcc cagcgcgtgc
     7981 cgtcggccag ttgggcgtcc agatcgttgg cgctgtgctg caagccgcgc aaatgctggt
     8041 gcagaccgcg caagcgggct tcgctggcgg ggtcggtcgc gggcaaggtg gcgcgctggg
     8101 cggccagccg ggcctggaga ctatcaggag gtttgatgat catgctggct gtgcgccaca
     8161 ctcagcgccg gcgcaccgag ctggtgacga tattgacggt cagctgatac agctggcctt
     8221 gttgcacttc caggcagcat acggcttcga cttcggtggt gaagggctcg ctggggcggc
     8281 caccgatatc gggcggatag acggtcagct cgatgcgttc gccctgctgc ggcacatacg
     8341 gcgtgcatac ctgcatgccg gtgatgcgca cgtcgtagca cagtgccggg taagtctgat
     8401 tggtgaccag cgagcgcagc ttgccctggc agtgcggcgg cacgtcctta ttgccggcgt
     8461 gcgcgccaga gtagcctggc tggcggcctg ggctgtgctg atgggaatgg tgatggttgt
     8521 taaagcggct cactgcgcaa catcccagat tgccaggctt gcagccagaa caggccggtc
     8581 agcgttttgc tgtcgctgag ctcgccgcgc tgggcgcggg cgatcacttc ggtcagcggc
     8641 aggtgttcca cttcgacaaa ctcgccttcg tctggctggc tgtcgccagc aatcaggcct
     8701 tcagccacaa aataggcgat tttttcgtct gaatagccaa tgcacggatg cgccactggc
     8761 agcgcatgcc agcggcgggc ggtgtagccg gtttcttcca gcagttcgcg ctgggcggtg
     8821 gccagccagg cctcgccagc gtcgatcttg ccggcgggga tttccagaaa ggtctggcgc
     8881 accggatagc gatactggcg caccagcacc agctcgccgg cctcggtcag cggcaaaatc
     8941 gccaccgcgc cggggtgggc gatgtactcg cgccgcgcct cgctgccatc gggcaggcgc
     9001 acgcgatccc gatacacgcg caaaaagccg ccttcgagca gttgttccga atccagtgtg
     9061 tgttcagtca gagccatgtg atgttttcct tgcgtgccgc cgatgccgcc aagcgtgcac
     9121 agtatgccat cgccatgcca gcatcaccag tccatggccg gccaggccca gggtcagtcc
     9181 ctgcgccggc acgcccagca ataatggcca gcccagggtt tgcgcccaat ctaccatctg
     9241 ccccagccag acgccaaatc cctgcgtagc ccactcgggc ggcagcgctg gcaggccgcc
     9301 ttgctggccg gtgagcagac agccatattc ataagccagc cagtataaag gaacaatggt
     9361 cagcgggttg gtgtacagcg tggtggccag cgccaccggc agattgatgc gccagcacca
     9421 ggccagcgcg ccggctgtca gcatctgtgt gggcccgggc atcagcccgg caaacaagcc
     9481 caccgccacc gcgcgcgcca cattgcgtcg gtgcagcgtc cacagctgcg gatcttgcag
     9541 gcgtgggccc atccaggcca gccagcggtt ggcggccagg gtggccgggt cgggcagata
     9601 acgtttgaac cacttgcgcg gcatggtgat ttgtccagaa aaggctagct tatagagtag
     9661 ggtaggtgac cagattgggg tggctgaccg ttgttgcaat gcaacaacgg tggattgtcg
     9721 cgcgggaagt gtggctagaa acatacactt aaaacattcg tttgaaaaaa gattgccagc
     9781 cccggatttt gcccttacat ttaccccgcc aacacgccat ccagcaagga gggcgaacct
     9841 acacggagag acatcatgcc tgcatacaaa gccccgctgc gcgattttga ttttgtcctg
     9901 aacgaagtga ttcaggtgca aaacgtgatt tccaccctgc ctggctacga agaagccacc
     9961 caggacgtgt ttgcctccta tctggatgcc gcggccaagt tctgcgaaaa cgaactggcc
    10021 ccgatcaacc gcgccgccga cgaagagggt tgcagcttca atccggctga ccacagcgtc
    10081 accaccccgg ccggcttcaa agaagcctac caacaatact gcgaactggg cttcaccggc
    10141 ctggattgtg acccggccta cggcggccag ggcatgccca aggtgctggc tttcccgatg
    10201 atggaaatgc aatgctcggc caacgtcgcc tggtcgatgt accccggcct gagccacggc
    10261 gcctacgccg ccatccacgc ccacggcact gacgaacaaa aagccaccta cctgcccaag
    10321 ctggtggacg gcagctggac cggcaccatg tgtctgaccg aaccgcactg cggcaccgac
    10381 ctgggcctgc tgaaaaccaa ggccgagccg aatggcgacg gcagctacag catcaccggc
    10441 accaagatct tcatctcggc cggcgaacac gacatgagcg acaacatcat tcacctggtg
    10501 ctggcccgcc tgccggattc gcccaaggac gtgaagggca tctcgctgtt catcgtgccg
    10561 aagttccacg ccgacggcag ccgcaacccg gtgttctgcg gcgcgctgga acacaagatg
    10621 ggcatcaagg ccaacgccac cgcggtgatc aacctggacg gcgccaaggg ctatctgatt
    10681 ggcgaacgcg acaagggcct ggcctgcatg ttcaccatga tgaacgccgc ccgtctgggc
    10741 tgcggcatgc aaggcctggg cctgggcgaa gcttcgttcc agggcgcgct gagctatgcc
    10801 cgcgaacgcc tgcaaatgcg cgcgctgacc ggcccgaaag ccccggaaaa agccgccgac
    10861 ccgatcatcg tccacccgga cgtgcgccgc atgctgctga cgcaaaaagc ctacaccgag
    10921 gccggccgcg ccatgaccgc ctggctggcg ctgcaactgg acatcgaaga aaaatccccg
    10981 gacgccgacg cccgcagcca ggccgctgat ctggtggcgc tgctgacccc ggtggccaag
    11041 gccttcatga ccgacaatgg ctacaccgcc gccaatatgg gcatgcaggt gtttggcggc
    11101 catggcttta tccgcgaatg gggcatggaa cagctggtgc gcgattgccg catctcgcaa
    11161 atctacgaag gcaccaacgg cattcaggcc atcgacctgc tgggccgcaa ggtgctgatg
    11221 gaccaaggcc agaagctgcg caagttcacc aagatcgtgc acaagtactg cgaagccaac
    11281 ggcggagatt cgcgcctgaa agacatggcc gaagcagtga gcaagctgct gaaggatgtg
    11341 ggcgatgtca ccatgcacgt cggcatgcag tcgatgatga accgcgacga agccggcgcc
    11401 gccgccaccg actacctgcg cctgattggc cacctgacct atggcttctt ctggctgtgg
    11461 atcagcaaga ccgcgctgga caagctggac gccggcacgg gtgagaaagc cttctacgaa
    11521 gccaagctga ccaccgcgcg cttctacttt gccaagctgt tcccggaagt gcacagcctg
    11581 ctggtcaccc tgaaagc
//
LOCUS       DAOPYX010000056        11590 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_6338_length_11590_cov_10.253143, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000056 DAOPYX010000000
VERSION     DAOPYX010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 11590)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11590)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11590
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6338_length_11590_cov_10.253143"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(134..1516)
                     /locus_tag="PKN55_04480"
     CDS             complement(134..1516)
                     /locus_tag="PKN55_04480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008953009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015514 - nitrite efflux transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015707 - nitrite transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NarK family nitrate/nitrite MFS transporter"
                     /protein_id="HNX70531.1"
                     /translation="MSSRIITRWEPEHPTFWQQEGKAVARRNLWISIPALTLAFIIWQ
                     VWSVAVLNMPNIGFRYSENQLFWLAALPALSGATLRIFYSFMVPVFGGRKWTALSTAS
                     LLIPAIGIGFAVQDPNTSYATMAGLALLCGFGGGNFSSSMANISFFFPKAEKGTALGL
                     NAGLGNLGVSVVQFVVPIVITLGLFGALGGEPQTWVKGAVTKQMWLQNAGFVWVPFIV
                     LSTLAAWFGMNDLASAKASFADQAVIFKRKHNWLMCWLYIGTFGSFIGFAAGFPLLVK
                     GQFPQIDPSKYVFFGPLVGALARPIGGWLSDKIGGAKVTQTVFVLMVAAVFGVLQFLP
                     HGGQGGNFQGFFAMFLCLFALTGIGNGSTFMQVPAIFLTLHKRMAKPGDAAQKQAAAD
                     ATKEAAAVLGFSGAIGAYGGFFIPKSYGTSIALTGGVEAALYAFIAFYLSCLLINGWY
                     YARKNAEVPC"
     gene            complement(1605..2858)
                     /locus_tag="PKN55_04485"
     CDS             complement(1605..2858)
                     /locus_tag="PKN55_04485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter"
                     /protein_id="HNX70532.1"
                     /translation="MASDPTQAGNARQWSVLAVSTLSFTVCFMVWMMFGVVGIPLKQQ
                     LGLNETEFGILAATPVLTGSLVRVPLGIWTDRFGGRVVFFLLMLSTVLPIYLMEYATA
                     YWHFLAIGLFVGLAGGSFSVGTPYVARWFSRDKQGLAMGVFGAGNSGAAVNKFAAPAL
                     IAAGGWMLVPKVYAGVMLVTAILFWLFSAHNPQHTVGKSVRFADQLKVMGDRRVWRYS
                     QYYSVVFGGYVGLALWMTKYYVGEYGFDMKLAAFLAACFSLPGGVLRAIGGWMSDKYG
                     AYKTTWWVMWVLWVCFFILSYPQTALVIQTVKGPMSLNIGLSPWLFTSVMAVAGVAMA
                     VGKASVFKFISDDFSDNIGAVSGVVGLAGGLGGFILPIMFGALLDLTGVRTSCFMLLY
                     GTVCVSLVWMHYSFKPALLSNRQAA"
     gene            3113..4087
                     /gene="moaA"
                     /locus_tag="PKN55_04490"
     CDS             3113..4087
                     /gene="moaA"
                     /locus_tag="PKN55_04490"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014086649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HNX70533.1"
                     /translation="MLQDRFGRAVEYVRLSVTDRCDLRCSYCIPKGFKDFAEPEDWLT
                     FAEIERVIGAFVRLGARRVRLTGGEPLLRRGVVDLAARLAALPGLRDLSVSTNGTQLA
                     RLAGPLKAAGVGRVNVSLDSLRRECMAEVTGCDSLPQVLDGLKAAKAAGLTPIKLNMV
                     LLPGVNDQDVESMVAFCIEHGFILRLIEAMPMGDSGRSVGYLDAQGVLARLRARFDLR
                     PQTVSLGGGPARYWATADGRFTLGVISPISQHFCATCNRVRLSVDGVLYMCLGQEESV
                     ELRPLLRAGISDADLQQVLRQAIELKPERHVFASAPGKVVRIMAKTGG"
     gene            4157..4810
                     /locus_tag="PKN55_04495"
     CDS             4157..4810
                     /locus_tag="PKN55_04495"
                     /EC_number="4.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018151846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HNX70534.1"
                     /translation="MPSIEHFLSGFRRFQQYYYLDNDSLFTDLKRGQHPSTLLVGCCD
                     SRVDPLMLTGCEPGELFIVRNVANLVPPCQEDSHYHGVSAALEYAVISLQVERIIVLG
                     HASCGGIRALMEGITDHQEDGGFLAKWLSIADPAREFVLAHHANDNVATQLAVAERVS
                     ILVSLDNLMSFPWVRNRVETGKLQLLGWYFDIQEGALYGFDAHSKRFTPLVCPLGAV"
     gene            4807..6747
                     /locus_tag="PKN55_04500"
     CDS             4807..6747
                     /locus_tag="PKN55_04500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014086650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pili methyl-accepting chemotaxis
                     transducer N-terminal domain-containing protein"
                     /protein_id="HNX70535.1"
                     /translation="MSDEAALPAGALPLSQRLTVKILFSFISFLLLALAAIGATLLLS
                     WQLEGGAAAINDAGSLRMRTYQLGLQLRQVEQDPARAVQLSANLGRFEQTLQQLEQGD
                     PARPLLVPRRADILAELDEVRTHWSVRMRPLLLSSAEQTLNRTVRGDAFEREIEPFVG
                     KIDTLVRDIEQHNARKTEWLRLCQTLLTTLAVLGTVALIYLMFLIVIRPLERLHLGIQ
                     RLASGDFSSRVPVETHDEFGEVAAGFNDMAGRLAEANATLEFQVEAKTAKLTEKNREL
                     SLLYEIAAFLNRPATLEELSQGFLERVSAAFAADGGAVRLSDTPHGMLFLVAQHGLPA
                     GLSEREHCLPLGECVCGEAAASGETRIETFTAGDGRFDCQREGFVVVSSFAISSQARL
                     LGVYHLHFRQPRIFTRQQRQLLETLGQHLAAALDNQRLAATARELAVLEERNLVAQGL
                     HDSIAQGLSFLNLQTQMLEDALRRDDREALAESLGQIQAGVQESYDDVRELLTNFRAS
                     LAAEDLFSAVDAILERFRRQTGLRVEVQREGDGAPLSPDQQLQVLFILQESLSNVRKH
                     ARASRVVVRIEDERDLLLTVRDDGVGFDAQEVAQRPSGHFGLSIMQERAQRAGAELTI
                     GPAAPYGTQVVVRLAQRDRLSA"
     gene            6961..7626
                     /locus_tag="PKN55_04505"
     CDS             6961..7626
                     /locus_tag="PKN55_04505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011136082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNX70536.1"
                     /translation="MTSPIRVLLVDDHTLFRSGVRALLARQAEIDVVGEAENGPEGVK
                     LAASLNPDVVLLDLNMPGLSGVETLQLLLQDAPQRAVVMLTVSEDADDLARALRAGAR
                     GYLLKNINADFLVNAIRRAADGESVMSEAMTAKLMAQFRQGGASPAPAVSEADKLTPR
                     EREILVFIARGDSNKEIARTLDLAESTVKIHVQNILKKLSLSSRVQAAVFAVEHGLAD
                     SRP"
     gene            7778..8953
                     /locus_tag="PKN55_04510"
     CDS             7778..8953
                     /locus_tag="PKN55_04510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014085833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA C-terminal
                     FtsZ-binding domain-containing protein"
                     /protein_id="HNX70537.1"
                     /translation="MSDFQLGILALGAVVIGAVFGYNWFQERRYRQQTKTAFEREHPD
                     VLLDVPGRDGKGERLEPALDDDMPNPLDPAPHKESAGSTQADTLIRYAQANPASEAAP
                     DVADMTEMSEDELDQSLSSIEQQLLIATLLDPMLDFIAEVRFEQPVTLPRLPSFAIDK
                     RVEMLGNNPDSGWERARANRSYSQLNIGVQMVDRRGPLNESELNAFCSQAQDFAEQHG
                     SNASFPQRQTRLTAARELDQFCAGVDVMIGLNLLSAQPFDGERLRALAESAGMQLEAD
                     GHFHYLSDSGNVLFMLGNANGQPFSQDSLAHDDFSALTLLFDVPRVAGGISVFDRAVA
                     LARQLAGELDAQLIDDNQRPLTESGINTIRHQLQQLYSRMDDRGIAPGSVAALRLFA"
     gene            8950..11379
                     /gene="ligA"
                     /locus_tag="PKN55_04515"
     CDS             8950..11379
                     /gene="ligA"
                     /locus_tag="PKN55_04515"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014085834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HNX70538.1"
                     /translation="MTQTSLFAARAAELRALLFQYGREYYVLDAPSVPDAEYDRLFRE
                     LQALEAAHPELRSDDSPTQRVGGEALAEFAPVVHAVPMLSIRTETDVTAAGAEAFDAS
                     VRRELDLLPLDAPIDYAVELKFDGLAISLRYQDGVLTQAATRGDGATGEDVTQNIRTI
                     GQIPLRLNGAAPALLEVRGEVYMRRDHFEALNAGLLARGEKPYVNPRNTAAGAVRQLD
                     PKITAQRPLSFFAYGLGQVTGWDAMPDTHAGVLDALAAFGLPVCEVREVVAGAAGLAA
                     FHQRVAELRDSLPFDIDGVVYKVNSLAVQRQLGFRTREPRWAVAHKYPAQEALTVVEA
                     IDVQVGRTGALTPVARLRPVFVGGVTVTNATLHNEDEVQRKDVRVGDTVVVRRAGDVI
                     PEVVSVVLAQRPMQALPGADLFSEGDSAPLHPPYRLPSHCPVCGSHTVREPGEAITRC
                     AGGLSCRAQRSQAIQHFAGRRMMDIDGLGERYIDSLVEFELVRGVAGLYALDLDKLLD
                     MKRRADERDNCVPDTVKAGKVATKWADNLLQAIAASKTPPLARLLFALGIRHVGESTA
                     KTLADCLGQLDWVRRAPAAALAALPDIGAVVADSIAGFFAEPGNQAEVDALLAAGVAP
                     QQAAHPSTKLHGLFEPVTLLVKLAIPRLTELRAGQLLAQVADLYALAALNRAQVIALD
                     LPAEAANALADWLDKPTQRAALAQLADYRRDILAAIPAEAEQAAAPQPLAGKTVVLTG
                     TLPTLSRDEAKVRVEAAGGKVSGSVSKKTDFVVAGEAAGSKLDKAQALGVPVLDEAAL
                     LALLAAPDSSD"
     gene            11404..>11590
                     /locus_tag="PKN55_04520"
     CDS             11404..>11590
                     /locus_tag="PKN55_04520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HNX70539.1"
                     /translation="MQAIRKAVFPVAGMGTRFLPATKASPKEMLPIVDKPLIQYAVEE
                     ALEAGMTDLIFITGRNKR"
BASE COUNT         2125 a         3658 c         3766 g         2041 t
ORIGIN      
        1 gccactggcc gatctgcccg cataaaaaac ggcgcccggg ggcgggcgcc gcaatgcttg
       61 tgtggacaag ccggagaaac cgcccaccca cggggcggac ggatgacaag accgatattg
      121 cctggcgcag cgctcagcag ggcacctcag cgttcttgcg ggcgtaatac cagccattga
      181 tcagcaggca gctcagatag aacgcaataa acgcatacaa ggccgcttcc acgccgccgg
      241 tcagcgcaat cgaggtgccg tagcttttcg ggatgaaaaa cccgccatag gcgccaatcg
      301 cgccggagaa gcccagcacg gcggcggctt ctttggtggc gtcggcggcg gcttgttttt
      361 gcgcggcgtc gcccggcttg gccatgcgct tgtgcagggt gaggaagatg gccggcactt
      421 gcataaaggt cgagccgttg ccgatgccgg tcagggcgaa caggcacagg aacatggcga
      481 aaaagccctg gaagttgccg ccctggccgc cgtgcggcag aaattgcagc acgccaaaca
      541 ccgccgctac catcagcaca aacaccgttt gtgtgacctt ggcgccgccg attttgtcgg
      601 acagccagcc gccaatcggc cgggccagcg cgcccaccag cgggccgaag aacacatatt
      661 tgctcgggtc aatctgcggg aactggcctt tcaccagcag cggaaaacca gcagcaaagc
      721 cgatgaacga accaaaggtg ccgatgtaca gccagcacat cagccaattg tgcttgcgct
      781 tgaaaatcac cgcctgatcg gcaaacgagg ccttggccga ggccaggtca ttcatgccaa
      841 accaggcggc cagggtggac agcacaataa acggcaccca gacaaaaccg gcgttttgca
      901 gccacatctg cttggtgacc gcgcccttca cccaggtttg cggctcgccg cccagcgcgc
      961 caaacaggcc cagggtgatg acgatgggca cgacaaactg caccaccgac acgcccagat
     1021 tgcccaggcc ggcattcagg cccagcgccg tgcctttttc ggccttggga aagaaaaagc
     1081 tgatattggc catcgacgag ctgaaattgc cgccgccaaa gccgcacagc agcgccaggc
     1141 cggccatggt ggcgtagctg gtgttggggt cttgcacggc aaaaccaatg ccgatggccg
     1201 gaatcaacaa actggcggtg gacagcgccg tccacttgcg cccgccaaac accggcacca
     1261 tgaaggaata aaaaatccgc agcgtggcgc cagacaaagc cggcagcgcc gccagccaga
     1321 acagctgatt ctcgctgtag cgaaagccga tattgggcat gttcagcacc gccacgctcc
     1381 acacctgcca gatgatgaag gccagggtga gcgcggggat ggaaatccac agattgcgcc
     1441 gggcaacggc tttgccctct tgctgccaga aagtcggatg ctccggctcc cagcgggtga
     1501 tgatgcgact ggacatggaa agtctctcca cttgcgagcg cggggcggcc gccccgctgg
     1561 ttggccgccg gcgcgcacag gccagcggcg gatgggtgag ggggtcaggc cgcctgccgg
     1621 ttgctgagca aggcgggttt gaacgagtag tgcatccaca ccaggctgac gcacaccgtg
     1681 ccgtacagca gcatgaagca gctggtgcgc acgccggtca ggtccagcag cgcaccgaac
     1741 atgatcggca agataaaacc gcccaggcca ccggccaggc cgaccacgcc agacaccgcg
     1801 ccgatgttgt cgctgaaatc atccgagatg aacttgaaca cgctggcctt gcccacagcc
     1861 atcgccaccc cggccaccgc catgacgctg gtgaacagcc acggcgacag gccgatgttc
     1921 aggctcatcg ggcctttgac ggtttgaatc accagcgcgg tttgcgggta agacaaaata
     1981 aagaagcaca cccacagcac ccacatcacc caccaggtgg ttttgtaggc gccgtatttg
     2041 tccgacatcc agccgccaat ggcgcgcagc acgccgccgg gcagcgagaa acaggccgcc
     2101 agaaacgccg ccagcttcat gtcaaaaccg tattcgccga cgtaatactt ggtcatccac
     2161 agcgccaggc cgacatagcc gccaaacacc accgagtaat actggctgta gcgccacacc
     2221 cggcggtcgc ccatcacttt cagttggtcg gcaaagcgca cgctcttgcc cacggtgtgc
     2281 tgcgggttat gcgcgctgaa cagccagaac aaaatcgccg tcaccagcat caccccggcg
     2341 tacaccttgg gcaccagcat ccagccgcca gcggcaatca gcgccggggc ggcgaatttg
     2401 tttaccgccg cgcccgagtt gcccgcgcca aacacgccca tggccaggcc ttgcttgtcg
     2461 cgggaaaacc agcgcgccac atacggcgtg cccaccgaaa acgagccgcc ggccaggccg
     2521 acaaacaggc caatggccag aaaatgccag taggcggtgg catactccat cagataaatg
     2581 ggcagcacgg tggacagcat cagcaagaaa aacaccacgc gcccgccaaa gcggtcggtc
     2641 cagatgccca gcggcacgcg caccagcgag ccggtcagca ccggggtggc ggcgaggatg
     2701 ccgaattcgg tttcattcag gccgagttgt tgtttcagcg gaatgccgac gacgccaaac
     2761 atcatccaca ccatgaagca cacggtgaat gacaaagtgc tgacggccag caccgaccat
     2821 tgccgggcat tgccggcctg tgtggggtcc gaagccatga tgcttccttt ggtgatcaat
     2881 catggttcga gcttacgagc cggccgccca cccgcccatt ggccgaacag tgggcgcagt
     2941 ggggtttttg gaactagcgg ggctagtccg tttgggtgag cgggtggggc atggctgcct
     3001 cgccggcagc ccgccctagt tccaatggct aatcccccac cctgccgatg gccgactgtg
     3061 gcgcggccgg cgctggccta tcatcggtct atcagtcatc cggagtcgga acatgctgca
     3121 agaccgtttt ggccgggccg tggaatatgt gcgcttatcg gtcaccgacc gctgcgactt
     3181 gcgttgcagc tactgcatcc ccaaggggtt caaagacttt gccgagccgg aggactggct
     3241 gacgtttgcc gagatcgaac gggtgattgg cgcgtttgtg cggcttggcg cccggcgggt
     3301 gcggctgacc ggcggcgagc ccttgctgcg ccggggcgtg gtggatctgg ccgcgcggct
     3361 ggcggccttg ccgggcctgc gcgatttgtc ggtgtccacc aatggcaccc agttggcgcg
     3421 gctggccggg ccgttgaagg cagccggggt ggggcgggtg aatgtcagcc tggattcgct
     3481 gcgtcgcgaa tgcatggccg aggtcactgg ctgcgatagc ctgccgcagg tgctggacgg
     3541 gctgaaagcc gccaaggcgg ccgggctgac gcccatcaag ctgaatatgg tgctgctgcc
     3601 gggagtgaat gatcaggatg tggaatccat ggtggcgttc tgcatcgaac acggctttat
     3661 cttgcggctg attgaagcca tgccgatggg cgattctggc cgcagcgtcg gctatctgga
     3721 cgcacaaggc gtgctggcgc gcttgcgggc gcggtttgat ttgcgcccgc aaaccgtcag
     3781 cctgggcggc gggccggcgc gctattgggc caccgccgat ggccgcttta ccctgggggt
     3841 gatttcgccg atttcccagc acttttgcgc cacctgcaac cgcgtgcgcc tgtcggtgga
     3901 tggcgtgttg tatatgtgtc tgggccagga agaaagcgtg gaattgcgcc ccttgctgcg
     3961 cgccggcatc agcgacgccg acttgcagca ggtgttgcgc caggcgattg agctcaagcc
     4021 ggagcggcat gtgtttgcca gtgcgccggg caaggtggtg cgcatcatgg cgaaaaccgg
     4081 cggctgaaat cgtccgggtt ctcgcgtatg cttgccgcgc cccggtttta ttgccatgac
     4141 tttcccctga gccctcatgc catccattga acattttttg tctggctttc gccgtttcca
     4201 gcagtattac tatctggaca acgacagcct gtttaccgac ctcaagcgcg gccagcatcc
     4261 ttccaccctg ctggtgggct gctgtgattc gcgggtggac cctttgatgc tcaccggctg
     4321 cgagccaggc gagctgttta ttgtgcgcaa cgtcgccaat ctggtgccgc cgtgccagga
     4381 agacagccat tatcacggcg tgtccgccgc gctggagtac gcggtgattt ctttgcaggt
     4441 cgagcgcatc atcgtgctgg gccacgccag ttgcggcggc atccgcgcgc tgatggaagg
     4501 catcaccgac catcaggagg acggcggctt tctggccaag tggctgagca ttgccgaccc
     4561 ggcgcgcgag tttgtgctcg cgcatcacgc taacgacaat gtggccaccc agttggcggt
     4621 ggccgagcgg gtgtcgattt tggtcagcct ggataatctg atgagctttc cctgggtgcg
     4681 caaccgggtg gaaaccggca aactgcaatt attgggctgg tactttgata ttcaggaggg
     4741 cgctttgtac ggctttgatg cgcacagcaa gcgctttacc ccgctggtct gcccactggg
     4801 ggcggtgtga gcgacgaggc cgcactgccc gccggcgccc tgccgctgtc gcagcggctg
     4861 acggtgaaga ttttgttcag cttcatcagc tttttgctgc tggcgctggc ggcgattggc
     4921 gccacgctct tgctgtcctg gcagctggaa ggcggcgcgg cggcgatcaa cgacgccggc
     4981 agcctgcgca tgcgcaccta tcagctgggc ctgcaactgc gccaggtcga gcaagacccg
     5041 gcgcgcgcgg tgcagttaag cgccaacctg ggccgctttg agcaaacctt gcaacaactg
     5101 gagcagggcg acccggcgcg cccgctgctg gtgccgcgtc gggcggatat tctggccgag
     5161 ctggacgagg tgcgcacgca ctggagcgtg cgcatgcgcc ccttattatt gtccagcgcc
     5221 gagcaaacgc tgaatcgtac cgtgcgcggt gacgcatttg aacgcgagat cgagcctttt
     5281 gtcggcaaga tcgacaccct ggtgcgcgat atcgagcagc acaatgcccg caaaaccgaa
     5341 tggctgcggc tgtgccagac cttgctgacc accctggcgg tgctgggcac ggtggcgttg
     5401 atttatctga tgttcctgat tgtcatccgc ccgctggagc gcctgcacct gggcattcaa
     5461 cgcctggcga gcggcgattt ttccagccgg gtgccggtgg aaacccacga cgaatttggc
     5521 gaagtggccg ctggctttaa cgatatggcc ggccgcctgg ccgaggccaa cgccacgctg
     5581 gaattccagg ttgaggccaa aaccgccaaa ctcaccgaga aaaaccgcga actgtcgcta
     5641 ttgtatgaaa tcgctgcttt tctgaatcgc cccgccacgc tggaagaact cagccagggt
     5701 tttctggagc gagtcagcgc ggcgtttgcc gccgacggcg gcgcggtgcg gctgagcgac
     5761 accccgcacg gcatgctgtt tctggtggcc cagcacggcc tgccggccgg tttgtccgag
     5821 cgcgagcatt gtttgccgct gggtgaatgt gtgtgcggcg aggcggcggc cagcggcgaa
     5881 acgcgcatcg aaaccttcac cgccggcgat gggcgctttg actgtcagcg cgaaggcttt
     5941 gtggtggtgt cctcgtttgc catcagctcg caggcgcgct tgctgggcgt gtatcacctg
     6001 cattttcgcc agccgcgcat ctttacccgc caacagcgcc aactgctgga aaccctgggc
     6061 cagcacctgg ccgccgcgct ggacaaccag cggctggcgg ccaccgcgcg cgaactggca
     6121 gtgctggaag aacgcaatct ggtggcgcag ggcctgcacg acagcattgc ccagggtttg
     6181 tcttttttga atctgcaaac ccagatgctg gaagacgcgc tgcgccgcga cgaccgcgag
     6241 gcgctggccg agagcctggg gcaaatccag gccggcgtgc aggaaagcta cgacgatgtg
     6301 cgcgagctgc tgaccaattt tcgcgccagc ctggcggcgg aggatttgtt cagcgcagtg
     6361 gacgccattc tggagcgctt tcgccggcaa accggcctgc gggtggaagt gcagcgcgag
     6421 ggcgacggcg cgccgctgtc gccggatcaa cagctgcaag tgctgttcat cctgcaagaa
     6481 tccttgtcca atgtgcgcaa gcacgcccgc gccagccggg tggtggtgcg catcgaggac
     6541 gagcgtgatc tgttgctgac cgtgcgcgac gatggcgtgg ggtttgatgc gcaggaagtc
     6601 gcccagcgcc ccagcggcca ctttggcttg tccatcatgc aagagcgcgc ccagcgcgcc
     6661 ggcgccgagc tgaccattgg cccggcggcg ccttacggca cgcaggtggt ggtgaggttg
     6721 gcgcagcgcg accggctcag cgcttagaac agctaacaaa accctcctgg cgtcgttgcg
     6781 cggccttgcc gtacaacccg cactgtctgc gcgcgcctgg ccaggaccgt tgcgctgggt
     6841 tttgtgagcc gcgcttggta gggatacgct gaaaaaacca tccctttccc tcgaagctgt
     6901 caatccagca tattgattgt gctgaatttt gttcaatcgt ttttttcagg caggttcgcc
     6961 atgacttctc ccattcgcgt gctgttggtc gatgaccaca ctttattccg cagcggtgtg
     7021 cgcgccctgc tggcccgtca ggccgaaatc gacgtggtcg gcgaggcgga aaacggcccc
     7081 gaaggcgtca aactggcagc ttcactcaac ccggacgtgg tgttgctgga cttgaacatg
     7141 cccggtttat ccggggtgga aaccctgcaa ctgttgttgc aggatgcgcc gcagcgggcg
     7201 gtggtgatgc tcaccgtctc ggaagacgcc gacgacctgg cccgcgcgct gcgtgctggc
     7261 gcgcgcggct atttgctgaa aaacatcaac gccgattttt tggtgaacgc catccgccgc
     7321 gccgccgatg gcgaatcggt gatgagcgag gcgatgaccg ccaaactgat ggcgcaattc
     7381 cgccagggcg gcgccagccc ggcgccggcg gtcagcgaag ccgacaagct caccccgcgc
     7441 gagcgggaaa ttctggtgtt catcgcccgt ggcgacagca ataaagaaat cgcccgcacc
     7501 ctggatctgg ccgaaagcac ggtgaagatt cacgtgcaaa acatcctgaa aaaactcagc
     7561 ctcagcagcc gggtgcaagc ggcggtgttt gccgttgagc atggtttggc tgacagccgg
     7621 ccctgacatc gggcacaatg ccggcctttg ccgccagcgc cgtctgcggt tttacatgga
     7681 taaacccttg tgatagcggt gaaacgcttc gcacgacgga tgcattggtt ataatggcgg
     7741 cgacaacaac acaacccatc agggtcggta gcggcgaatg agtgacttcc aactcggcat
     7801 ccttgcgctg ggcgcagtgg tgattggcgc ggtattcggt tacaactggt tccaggagcg
     7861 gcggtaccgc caacagacaa aaactgcgtt tgaacgcgag catccggacg tgctgctgga
     7921 cgtgcccggc cgcgacggca aaggcgagcg cctggagccg gcgctggacg acgacatgcc
     7981 caacccgctg gatccagccc cgcataaaga atcggccggc tcgacccagg ccgatacgct
     8041 gattcgctac gcccaagcca atccggccag cgaagctgcg cccgatgtgg ccgacatgac
     8101 cgaaatgagc gaggacgagc tggatcagtc gctgtcgtcg attgagcagc aactgctgat
     8161 tgccaccctg ctcgacccga tgctggattt catcgcggaa gtgcgctttg agcagccggt
     8221 cacgctgccg cgcctgccgt cgtttgccat cgacaagcgg gtggaaatgc tgggcaacaa
     8281 cccggacagc ggctgggagc gtgctcgcgc caatcgctcg tacagccaac tgaacatcgg
     8341 cgtgcaaatg gtcgatcgcc gtggcccgct gaatgaatcc gaactgaacg ccttttgctc
     8401 gcaagcccag gattttgccg agcagcacgg ttccaatgcc agctttccgc aacggcaaac
     8461 ccggctgacc gccgcgcgcg agctggatca attctgcgcc ggtgtggacg tgatgatcgg
     8521 cctgaacctg ctgtcggccc agccgtttga cggcgagcgc ctgcgcgcgc tggccgaatc
     8581 ggccggcatg caactggaag ccgacgggca ttttcattat ttgtctgatt ccggcaatgt
     8641 gctgttcatg ctgggcaacg ccaatggcca gccattcagc caagacagcc tggcgcacga
     8701 tgacttctcg gcgctgacct tgctgtttga cgtgccgcgc gtggctggcg gcatctcggt
     8761 gttcgaccgc gccgtggcgc tggctcgcca actggctggc gagctggatg cacagctgat
     8821 cgacgacaac cagcggccgc tgaccgaaag cggcatcaac accatccgcc accagctgca
     8881 acagctctac agccgcatgg acgaccgcgg cattgcgccg ggcagcgtgg ccgctttgcg
     8941 cctgtttgca tgactcagac ctcactcttc gccgcgcgcg cagccgagct gcgcgcgttg
     9001 ctttttcagt acggccgcga atattacgtg ctggacgcac ccagcgtgcc cgacgccgag
     9061 tacgaccggc tgtttcgtga gctgcaggcg ctggaagccg cccatcccga gctgcgcagc
     9121 gatgattcgc ctactcagcg cgttggcggc gaagcgctgg ctgaatttgc cccggtggtg
     9181 cacgccgtgc cgatgctgtc gatccgcacc gaaaccgacg tcaccgccgc cggcgccgag
     9241 gcgtttgacg ccagcgtgcg ccgcgagctg gatttgctgc cactggatgc gcccatcgac
     9301 tacgccgtcg agctgaaatt cgacggcctg gccatcagcc tgcgctatca agacggcgtg
     9361 ctcacccagg ctgccacccg tggcgacggc gccaccggcg aggacgtcac ccagaatatc
     9421 cgcaccatcg gccagattcc gctgcggctg aatggcgccg cgccggccct gctggaagtg
     9481 cgcggcgagg tgtatatgcg ccgcgaccac ttcgaggcgc tgaacgctgg cctgctggcg
     9541 cgcggtgaaa aaccctatgt caatccgcgc aacaccgctg ccggcgcggt gcgccagctc
     9601 gacccgaaaa tcaccgcgca acggccgctg tcgttttttg cctacggcct gggccaggtg
     9661 accggctggg acgccatgcc cgacacccac gctggcgtgc tggacgcgct ggcggcgttt
     9721 ggtctgccgg tgtgcgaggt gcgcgaagtg gtggccggcg ccgccgggct ggcggcgttt
     9781 caccagcgcg tggccgagct gcgcgacagc ctgccgtttg acatcgacgg cgtggtgtac
     9841 aaggtcaata gcctggccgt gcaacgccag ttgggttttc gcacccgcga gccgcgctgg
     9901 gcggtggcgc acaaataccc ggcgcaagaa gcgctgaccg tggtggaagc catcgacgtg
     9961 caagtgggcc gcactggcgc gctgaccccg gtggcgcggc tgcgcccggt gtttgttggc
    10021 ggcgtcaccg tcaccaacgc cacgctgcac aacgaagacg aagtgcagcg caaggacgtg
    10081 cgcgtgggcg acaccgtggt ggtgcgccgc gccggcgatg tgattccgga agtggtcagc
    10141 gtggtgctgg cgcaacggcc gatgcaagcg ctgccggggg cggatttatt cagcgagggt
    10201 gacagcgcac cgctgcaccc gccttaccgc ctgcccagcc attgcccggt gtgcggcagc
    10261 cacaccgtgc gcgagccggg cgaggcgatt acccgctgcg ccggcgggct cagctgtcgc
    10321 gcccagcgca gccaggccat tcagcatttt gccggccgcc ggatgatgga catcgacggc
    10381 ctgggcgagc gttatatcga cagcctggtc gagtttgagc tggtgcgcgg cgtagccggg
    10441 ctgtacgcgc tggacctgga caaactgctg gacatgaagc gccgcgccga cgagcgcgac
    10501 aactgcgtgc cggacacggt caaggccggc aaggtggcca ccaagtgggc ggataattta
    10561 ttgcaggcga ttgccgccag caaaacgccg ccgctggcgc gcctgctgtt tgccctgggc
    10621 atccgccatg tcggcgaatc caccgccaaa accctggccg attgcctggg ccagctggac
    10681 tgggtgcgcc gcgcaccggc cgccgcgctg gcggcattgc cggatattgg cgcggtggtg
    10741 gccgactcca tcgccggctt ttttgccgag ccaggcaatc aggccgaagt cgatgcgctc
    10801 ttggccgctg gcgtggcgcc gcagcaggcg gcgcatccct cgaccaagct gcatggcctg
    10861 tttgagccgg tgaccttgct ggtcaagctg gccattcccc ggctgaccga gctacgcgcc
    10921 ggccagttgc tggcgcaagt ggcggatttg tacgcgctgg cggccttgaa ccgtgcccag
    10981 gtgatagcgc tggatttgcc ggccgaggcg gccaatgccc tggccgactg gctggacaag
    11041 cccactcagc gcgctgcgct ggcgcaattg gctgattacc gccgcgacat tctggcggcc
    11101 atcccggccg aggccgagca ggccgccgcg ccgcagccgc tggccggcaa gaccgtggtg
    11161 ctgaccggca ccctgcccac cttgtcgcgc gacgaggcca aggtacgggt ggaggctgcc
    11221 ggcggcaagg tgagcggcag cgtgtcgaaa aaaaccgatt ttgtggtggc gggcgaagcc
    11281 gccggcagca agctggacaa ggcgcaggcg ctgggcgtgc cggtgctgga cgaagccgca
    11341 ctgctggcct tgctggctgc ccctgattct tctgactgac cccgcttctg gagactgttt
    11401 ctcatgcaag ccattcgtaa agccgtgttt ccggttgccg gcatgggcac ccgctttctg
    11461 cccgccacca aggccagccc gaaggaaatg ctgcccatcg tggacaagcc gctgattcag
    11521 tatgcggtgg aagaagcgct ggaagccggg atgaccgatt tgatcttcat caccgggcgc
    11581 aacaaacgcg
//
LOCUS       DAOPYX010000057        11516 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_6389_length_11516_cov_11.548730, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000057 DAOPYX010000000
VERSION     DAOPYX010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 11516)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11516)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11516
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6389_length_11516_cov_11.548730"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1523)
                     /locus_tag="PKN55_04525"
     CDS             complement(<1..1523)
                     /locus_tag="PKN55_04525"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNX70540.1"
                     /translation="MFAFSRWSVKNKLLALFLMLNLITVTAYSVYGFILKSQGVVAEI
                     DSRLTASASAVPDVLGSDYMHRLTQRNAVSAEEYLLRMSQLGRYAAQINLAYLYVVKM
                     DNGKVVFLSDGAPEEEIKAGKFKKQFEEYTDASDGLKDALASGSMRFDEYTDQFGYFR
                     SVFIPRKLANGETVALCADIRIDHVRSLLLNNIIQSVLIGLASFIVGGLIAWWLTGIL
                     ARSLERIGADIQKVASERDLSRTIKIPSQDEIGRMADHLNSMLGEMRRAIEDAAEAAK
                     TNSSTAHVLKDTCAQLVTSAADSATRVEQVTRYNQNIRAEAETSATQARDLAGRISVL
                     DEKLAGSREQVEIMMEAVNRNAETSVGIAQRFDGLAQNIRDITQILARIATISDQTNL
                     LALNAAIEAARAGEAGRGFAVVADEVRKLATQTQSTLSETNEFVERVLATIETTTREV
                     SEQAAESRELVSASSTVIDTIVQTSQLMEEAADVVNHTATSAESIRHDIEAVGGELE"
     gene            1790..2428
                     /locus_tag="PKN55_04530"
     CDS             1790..2428
                     /locus_tag="PKN55_04530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012695567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase"
                     /protein_id="HNX70541.1"
                     /translation="MTHTSLPSGEALPPLYLASASPRRRELLAQIGLSCIQLPMDIDE
                     TVLPDEAPADYVLRLAAAKARAGWARVAAEGLPARPLLAADTTVTVDGEILGKPADAF
                     EARAMLQRLSGRSHEVLTAVAVCHGVRVDSALSVSRVELLPLTAAMLDGYIASGEPFD
                     KAGAYGIQGRAASFIAHLDGSYSGVMGLPLCETARLLAMHGLDILGSHGHEQ"
     gene            2418..3923
                     /gene="rng"
                     /locus_tag="PKN55_04535"
     CDS             2418..3923
                     /gene="rng"
                     /locus_tag="PKN55_04535"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019389834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HNX70542.1"
                     /translation="MNSESILLAQWQREVQRANEQILVNITPQETRVAVLEDGIVQEL
                     HIERAASRGLVGNIYLGKVRRVLPGMQSAFIEIGLERAAFLHIADILEQRQHPEAPAR
                     IENMLFEGQTVLVQVIKDPIGAKGARLSTQISLAGRFLVHLPQETHIGVSQKIDSEQE
                     RAQLKERLEALLPSGSPRGYIIRTSAETASDEELAADIGYLTKLWADIESRARISPAQ
                     TLLYQDLSLALRVLRDMVSECTEKIIVDSTENYGKMVEFAEDYVTQALPKITRYSGER
                     PLFEMHGIEAEIQKALARRVNLKFGGYLIIDQTEAMTTIDVNTGGFVGAKSFEDTIFK
                     TNLEATYSIARQLRLRNLGGIIIVDFIDMDREDHRQAVLLELAKALARDRTRVTLNGF
                     TSLGLVEITRKRTRESLAHVLCEPCPTCQARGEIKTAQTVCYEIQREIVREARQFEAK
                     GFRILAAQSVIDMFLDEESQSLAMLIDFIGKPISLAVESQYTQEQFDVVLL"
     gene            4018..4860
                     /gene="cysE"
                     /locus_tag="PKN55_04540"
     CDS             4018..4860
                     /gene="cysE"
                     /locus_tag="PKN55_04540"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009116651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HNX70543.1"
                     /translation="MNTATPVIEDPVWDAVRREAAASAADEPLLASFLHMTVLRHRTL
                     EAVLSFHLSTKLAATVMDSRALMELIDEALQQDDALRAAMRADIEACYVRDPACDAYS
                     TPLLYYKGFHALQAYRVTHWLWGQGRKTLALFLQNRISEVMGVDIHPAARIGCGVMLD
                     HATGFVVGETAVIGDNVSILQGVTLGGTGKESGDRHPKIGNGVLIGANATVLGNITVG
                     EGAKIGAGSVVLAPVPAHATVAGVPAKVVGNTGHDAPALSMDQHFDGDEGCTVCPVRG
                     PQHG"
     gene            4853..5347
                     /locus_tag="PKN55_04545"
     CDS             4853..5347
                     /locus_tag="PKN55_04545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018151549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF45 domain-containing protein"
                     /protein_id="HNX70544.1"
                     /translation="MAELRYLTGYPPQLIEQVAALRDQGRLGDWLQQRYPDGAHDIQS
                     DRALYDYAQALKQRGLKNTPPLAKVLYDSQQHPVRGTLGTHTRAARVQGGKLKTRHEI
                     RIGALFRDAPEPMLRMIVAHELAHLKESEHNKAFYQLCEHLAPGYAQLEFDARVWLIW
                     RESA"
     gene            complement(5432..5731)
                     /locus_tag="PKN55_04550"
     CDS             complement(5432..5731)
                     /locus_tag="PKN55_04550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX70545.1"
                     /translation="MNAKTLTQSLIAGVFAVSAGLGFAADAAKPEAKPAAAPAPAAAA
                     AAPAAKAEAKPEVKAEVKKTEKAEKKDVKKVEHKAEHKAEKKVEKTEAKPEAAKQ"
     gene            6070..7353
                     /gene="gshA"
                     /locus_tag="PKN55_04555"
     CDS             6070..7353
                     /gene="gshA"
                     /locus_tag="PKN55_04555"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008954810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HNX70546.1"
                     /translation="MPVPCLTTALTGPLRELETRILNAQPQIEHWFRSQWTQYTPPFY
                     GSVDLRNSGFKLAPVDMNLFPGGFNNLSQDFLPLAVQATQSALEKICPEARSVLLVPE
                     NHTRNTFYLRNVAALKHILELAGLKVRLGSLSPEVTAPTVLELGDGETLTIEPLVRHG
                     HRVSLGDDFNPCVVLLNNDLSAGVPEILRNVDQAVLPPLHGGWAVRRKSNHFAAYDQV
                     AHEFAELIGIDPWFINPLFNGCKGLDFHQRQGEEALAEVVDQMLGKIAAKYREHKIDA
                     KPFVIVKADAGTYGMGIMSVKDAREVIGLNRKQRNKMAVIKEGQEVSEVIVQEGVYTF
                     EQVNEAVAEPVVYMIDRYVVGGFYRVHTGRGVDENLNAPGMHFVPLSFESPCLPDRKG
                     SPDCPPNRFYAYGVVARLALLAGAVELDNTDPLRG"
     gene            7374..8315
                     /gene="gshB"
                     /locus_tag="PKN55_04560"
     CDS             7374..8315
                     /gene="gshB"
                     /locus_tag="PKN55_04560"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012698534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HNX70547.1"
                     /translation="MRIAFIADPLESFKIYKDSTFAMMEEAARRGHSLYALLAEDMYV
                     AAGVVRAKVSPISLTGHEDWFVRGAAEDCALLAFDAVVMRKDPPFDAEYLYATHLLSL
                     AETQGARVFNRGQALRDYNEKLAILQFPEWTTPTLVSSQPERLRAFVGEQGDTILKPL
                     DSMGGDGIFRVSPSDPNLSVILETMTLRGERTIMAQRYLPQIVDGDKRVLVIDGEPVP
                     WCLARIPKAGETRGNLAAGGRGEARELSARDREIAQALGPRLKADGLLLVGLDVIGEH
                     LTEVNVTSPTCFREIMDQTGLNVAGLFIDALERRCAA"
     gene            8303..9370
                     /locus_tag="PKN55_04565"
     CDS             8303..9370
                     /locus_tag="PKN55_04565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012698535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HNX70548.1"
                     /translation="MRRLSRLAGLLAALMLLAGCQREPTLWQQESYVFGTRVQITTAD
                     VPENVARPAVAAALADLDRWHVRLHAWRDDSELTRINRALAAGQPVDTDSEVLGLLRQ
                     AQDISARAEGLFNPAIGQLVALWGFHADSFAPVRPEPGALARLLASAPSMADVQFDGQ
                     RVSSRNRAVSFDLGGMAKGWALDRVADRLRQAGVKSALINIGGNILALGSKGDTPWKV
                     GLQAPRDNQAMAVIVLHDGEALGTSGDYQRYFELDGVRYCHLIDPRNGSAACQRQAAT
                     VLVEPGPDAGLRSDVASKPLFFAPRADIARLAGRFAIRDVLLVEADGHTWLSPAMQPR
                     VQWLQRPQQVDVLTGFAEAPT"
     gene            9544..9936
                     /locus_tag="PKN55_04570"
     CDS             9544..9936
                     /locus_tag="PKN55_04570"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008954813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004143 - diacylglycerol kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HNX70549.1"
                     /translation="MTGHTESPFKGKTGLRRLINALGYSLDGLAAAFRHEAAFRQLSL
                     LAAVLLPLAIWLPVTPIERALMMAAPLATLIIELLNSAIEAAVDHTSLDRHPLAKRAK
                     DMGSAAQLLGLINLAVVWGGILYGHYLA"
     gene            complement(10082..10528)
                     /locus_tag="PKN55_04575"
     CDS             complement(10082..10528)
                     /locus_tag="PKN55_04575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX70550.1"
                     /translation="MPTKTKWDSRFFLTGAGVLSVMFMLDGDWLLLPLALAVLLWGAR
                     QADSERVARLMSGEGDADALSMLFEPRRSHSLLPLDHFTCRDLMFYRAGWPVYRFLRS
                     SRCWFEFVGEREQVGALTEQHILVFPGVVYCKREDVALDEAPARQR"
     gene            11147..>11516
                     /locus_tag="PKN55_04580"
     CDS             11147..>11516
                     /locus_tag="PKN55_04580"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018748166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase domain-containing protein"
                     /protein_id="HNX70551.1"
                     /translation="MMIQTLRLTVLSSVLLSAAVMAADKPNVMILATGGTIAGTGATS
                     TTTVGYTAAKVGVERLIEAVPELKNVANVKGEQVFQIASENMTNDHWLKLSKRVNELL
                     AQRDVDGIVITHGTDTLEETA"
BASE COUNT         2075 a         3675 c         3644 g         2122 t
ORIGIN      
        1 tgctccagtt cgccgcccac ggcttcgatg tcatggcgga tgctttcggc gctggtggcg
       61 gtgtggttca ccacatcggc ggcttcttcc atcagctggc tggtttgcac gatggtgtcg
      121 atcaccgtgc tggaggcgct gaccagctcg cggctttcgg cggcctgctc ggacacttcg
      181 cgcgtggtgg tttcgatggt ggccagcacc cgctcgacaa attcattggt ttccgacagc
      241 gtggactggg tttgcgtggc cagtttgcgc acctcgtcgg ccaccacggc aaagccgcgg
      301 ccggcttcgc cggcgcgggc ggcttcgatg gcggcattca gcgccaggag gttggtctgg
      361 tccgagatgg tggcgatgcg cgccagaatc tgggtgatgt cgcggatgtt ttgcgccagg
      421 ccgtcaaagc gctgggcgat gcccacgctg gtttcggcgt tgcgattcac cgcttccatc
      481 atgatttcca cctgctcgcg cgagccggcc agtttttcgt ccagcacgct gatgcgcccg
      541 gccaggtcgc gcgcctgggt ggccgaggtt tccgcctcgg cgcgaatgtt ctggttgtag
      601 cgggtgactt gctcgacgcg ggtggcgctg tcggcggcgc tggtcaccag ctgggcacag
      661 gtgtctttca gcacatgcgc ggtgctgcta ttggttttgg cggcttcggc ggcgtcttcg
      721 atggcgcggc gcatttcgcc cagcatgctg ttgaggtggt cggccatgcg gccgatttcg
      781 tcctggctgg ggattttgat ggtgcgcgac agatcacgct cggaggccac tttctggatg
      841 tcggcgccaa tgcgctccag cgagcgcgcc agaatgccgg tcagccacca ggcgatcagc
      901 ccgcccacaa taaagctggc caggccaatc agcaccgatt gaatgatgtt gttcagcagc
      961 aggctgcgca catggtcgat gcggatgtcg gcgcacaggg cgacggtttc accattggcc
     1021 agcttgcgcg ggatgaacac cgagcggaag tagccaaatt ggtcggtgta ttcgtcaaag
     1081 cgcatcgagc cgctggccag cgcgtctttc aggccgtcgc tggcgtcggt gtattcttca
     1141 aactgctttt tgaacttgcc ggccttgatt tcttcttctg gcgcgccatc cgacaaaaac
     1201 accaccttgc cattgtccat cttgaccaca tacagatagg ccagattgat ttgcgcggcg
     1261 tagcggccca gctggctcat gcgcagcagg tattcctctg ccgatacagc gttgcgctgg
     1321 gtgagccggt gcatatagtc gctgcccagc acatcgggca ccgcgctggc cgaggcggtg
     1381 aggcggctgt cgatttcggc gaccacccct tgtgatttga ggataaagcc atacaccgag
     1441 taggcggtga cggtgatcag gttgagcatc aggaacaagg caagcagctt gttcttgact
     1501 gaccagcggg agaacgcaaa catggctgga ttccttctgg agaacgccgg catggcctgg
     1561 cgttttgcag cagccgggca tgctgataag ctaacgagaa accggccgga cgccaacagg
     1621 cgttgcacgg cgtctgaccg ggcttgccgt gacgcaatgc cgccatcgtg gcgcaaagcg
     1681 ctggcgggcc gtgatacacc tatgaccgat gtcggatgtc cctttgggat agactttggt
     1741 cacggtccgc caatataaaa caaaagcctg attcaggcca tgacaaatca tgacacacac
     1801 ctccctgcct tctggcgaag ccctgccgcc cctttatctg gcttcggcca gcccgcgccg
     1861 gcgcgaactg ctggcgcaaa tcggcctgtc ctgcatccag ttgccgatgg acatcgacga
     1921 aaccgtcctg cccgacgaag ccccagccga ctacgtgctg cgtctggccg ctgccaaggc
     1981 gcgcgccggc tgggcgcggg tggccgccga agggctgccg gcgcggcccc tgctggccgc
     2041 cgacaccacc gtgacggtgg atggcgaaat cctgggcaag ccggccgacg cctttgaggc
     2101 gcgcgccatg ctgcaacgct tgtccggccg cagccacgag gtgctcaccg ccgtggcggt
     2161 gtgccacggc gtgcgggtgg acagcgcctt gtcggtcagc cgcgtcgagc tgctgccctt
     2221 gactgccgcc atgctggatg gctacatcgc cagcggtgag ccgttcgaca aggctggcgc
     2281 gtatggcatt cagggccgcg ccgccagttt tatcgcccat ctggacggca gttactccgg
     2341 cgtgatgggg ctgccgctgt gcgaaaccgc gcgcctgttg gccatgcatg gcctggatat
     2401 tctggggagc catggccatg aacagtgaat ccattctgct ggcgcagtgg cagcgcgagg
     2461 tgcagcgcgc caacgaacaa atcctggtga acatcacccc gcaagaaacc cgggtggcgg
     2521 tgctggaaga cggcatcgtg caggaattgc acatcgagcg cgccgccagc cgggggctgg
     2581 tgggcaatat ttatctgggc aaggtgcgcc gcgtgctgcc cggcatgcaa agcgccttta
     2641 tcgaaatcgg cctggagcgc gcggcctttt tgcacattgc cgacattctc gaacagcgcc
     2701 agcacccaga ggcgccggcg cgcatcgaga acatgctgtt tgaaggccag accgtgctgg
     2761 tgcaggtgat caaagacccg attggcgcca agggcgcgcg gctgtccacc cagatcagcc
     2821 tggccgggcg ctttttggtg cacctgccgc aggaaaccca tatcggcgtt tcgcaaaaaa
     2881 tcgacagcga gcaagagcgc gcccagctga aagagcggct ggaagcgctg ctgccgtccg
     2941 gctcgccgcg cggctacatc atccgcacca gcgcagaaac cgcctcggat gaagagctgg
     3001 ccgccgacat tggctatctg accaaactgt gggccgacat cgaaagccgc gcgcgcatca
     3061 gcccggcgca aaccctgctg tatcaggatt tgtcgctggc gctgcgcgtg ctgcgcgata
     3121 tggtcagcga atgcaccgaa aaaatcatcg tcgactccac cgagaactac ggcaagatgg
     3181 tggagtttgc cgaggattac gtcacccagg cgctgcccaa gatcacccgc tacagcggcg
     3241 agcgcccgct gttcgagatg catggcattg aagccgaaat ccagaaagcc ctggcgcgcc
     3301 gggtgaacct gaaattcggc ggctatctga tcatcgacca gaccgaggcg atgaccacca
     3361 tcgacgtcaa caccggcggt tttgttggcg ccaagagctt tgaagacacc atcttcaaga
     3421 ccaacctcga agccacttat tccatcgccc gccagctgcg cctgcgcaat ctgggcggca
     3481 tcatcattgt cgattttatc gacatggacc gcgaagacca ccgccaggcg gtgctgctgg
     3541 agctggccaa ggcgctggcg cgcgaccgca cccgcgtcac cctgaacggc ttcaccagcc
     3601 tgggcctggt ggaaatcacc cgcaagcgca cccgcgaaag cctggcgcat gtgctgtgcg
     3661 aaccctgccc cacctgtcag gcgcgcggcg aaatcaagac cgcgcaaacc gtctgctatg
     3721 aaatccagcg cgaaatcgtg cgcgaggcgc gccagtttga agccaagggc tttcgcattc
     3781 tcgccgccca gtcggtgatt gatatgttcc tcgacgaaga atcgcaaagc ctggccatgc
     3841 tgatcgactt tatcggcaag ccgatttcgc tggcggtgga aagccagtac acgcaagaac
     3901 aattcgacgt tgtgctgcta taaactggcc ccatctcgct gttgatgtga cccgaaccgt
     3961 ccgccccgcc ggcacacctg ccggcggggc gcgccgcctt tgccggaacc ccgcctgatg
     4021 aacactgcca cacccgtgat tgaagacccc gtctgggacg ccgtgcgccg cgaagccgcc
     4081 gccagcgccg ccgacgaacc gctgctggcc agctttttgc acatgaccgt gctgcgccac
     4141 cgcacgctgg aagccgtgtt gagctttcat ttgtccacca aactggccgc cacggtgatg
     4201 gacagccgcg cgctgatgga gctgatcgac gaagccctgc aacaagacga cgccctgcgc
     4261 gccgccatgc gcgccgacat cgaagcctgc tatgtgcgcg acccggcctg cgacgcctac
     4321 tccaccccgc tgctgtacta caagggtttt cacgccctgc aagcctaccg cgtcacccac
     4381 tggctgtggg gccagggccg caagacgctg gcgctgtttt tgcaaaaccg catctctgaa
     4441 gtcatgggcg tggacatcca cccggccgcg cgcatcggct gcggcgtgat gctggaccac
     4501 gccaccggct ttgtggtcgg cgaaaccgcc gtgattggcg acaatgtctc catcttgcaa
     4561 ggcgtcaccc tgggcggcac cggcaaggaa tctggcgacc gccaccccaa aatcggcaac
     4621 ggcgtgctga ttggcgccaa cgccaccgtg ctgggcaata tcaccgtcgg cgaaggcgcc
     4681 aagatcggcg ccggcagcgt ggtgctcgcc ccagtgccag cgcacgccac cgtggccggc
     4741 gtgccggcca aagtggtggg caacaccggc cacgacgccc cggcgctgag catggaccaa
     4801 cactttgacg gcgacgaagg ctgcaccgtc tgcccggtgc gcggcccgca acatggctga
     4861 actgcgctac ctcaccggct acccgccgca actgatcgaa caagtggccg cgctgcgcga
     4921 ccaaggccgg ctgggcgact ggctgcaaca acgctacccg gacggcgcgc acgacatcca
     4981 gtccgaccgc gcgctgtacg actacgccca ggcgctgaaa caacgcggcc tgaaaaacac
     5041 cccgccactg gccaaagtgc tgtacgacag ccagcaacac ccggtgcgcg gcacgctggg
     5101 cacccacacc cgcgccgccc gcgtgcaagg cggcaaactg aaaacccgcc acgaaatccg
     5161 catcggcgcg ctgttccgcg acgcgcccga acccatgctg cgcatgatcg tcgcccacga
     5221 actggcgcac ctgaaagaaa gcgagcacaa caaggcgttc taccagctgt gcgagcatct
     5281 ggcgccgggg tatgcgcagc tggagtttga tgcgcgggtg tggttgattt ggcgggagtc
     5341 cgcctgatat cgcgctgtgg cagaacacgc aaaacccccg gcgcagagag cctgcggcgg
     5401 gggatgaaaa ctcagggcgc gggccgggcg cttattgctt ggcggcttcc ggcttggctt
     5461 cagtcttttc gactttcttt tccgctttgt gctcagcctt gtgttcaacc ttcttcacgt
     5521 ctttcttctc ggccttttca gtctttttca cttcggcctt cacttccggc ttggcttcag
     5581 ccttggcggc cggggcggca gcagcagcgg ccggggccgg cgcggcggcg ggtttggctt
     5641 ccggcttggc ggcgtcggcg gcaaagccca ggccggcgga cacggcaaac acaccagcga
     5701 tcagggattg ggtcagggtc ttggcgttca tggcggctat ccttttatct ttcacacatt
     5761 cagggtttgg gtttgccccc gaggcttcgg ggatcgctgc gttgtcgcag ccgatgagtg
     5821 aagattacgc cgccccctga gcggggtctg tgagcgatgg gtgtcggaat gtaactgcgt
     5881 gtacatgtcc gccttgaaaa aaccgttctc cccgccatct agcaattcag tttctggccc
     5941 gcctgacggt gaccccgcgt gtgaggccga aaaaaattgc aaccttgccg tgctaaaaat
     6001 caggtaagcc gggacgcggg atccgcgtgg ccggctgtct gattttctgc cgtccatgaa
     6061 aggcgcatca tgcccgttcc ttgcctgacc actgcgttga ctggcccgct gcgcgagctg
     6121 gaaacccgca tcctgaatgc ccagccgcag atcgaacact ggttccgcag ccagtggacg
     6181 caatacaccc cgccgtttta cggctcggtg gatttgcgca atagcggctt taagctggcg
     6241 ccggtggaca tgaacctgtt tccgggcggc tttaacaacc tgagccagga ttttttgccg
     6301 ctggcggtgc aggccacgca aagcgcgctg gaaaaaatct gtcccgaggc gcgcagcgtg
     6361 ctgctcgtgc cagaaaacca cacccgcaat accttttatc tgcgcaatgt cgccgcgctc
     6421 aaacacattc tggagctggc cgggctgaaa gtgcggctgg gcagcctgtc gccggaagtc
     6481 accgcgccca ccgtgctgga gctgggcgac ggcgagacgc tgaccatcga gccgctggtg
     6541 cgccacggcc atcgcgtcag cctgggcgat gatttcaacc cctgcgtggt gctgctgaat
     6601 aatgacctgt ccgctggcgt gcctgaaatc ctgcgcaatg tcgatcaggc agtgttgccg
     6661 ccgctgcatg gcggctgggc ggtgcggcgc aagagcaacc actttgccgc ttacgatcag
     6721 gtggcccacg aatttgccga gctgattggc attgatccgt ggtttatcaa cccgctgttc
     6781 aacggctgca aggggctgga ctttcatcag cgccagggcg aagaagcgct ggccgaggtg
     6841 gtggatcaga tgctgggcaa gattgccgcc aaataccgcg aacacaagat cgacgccaag
     6901 ccgtttgtca tcgtcaaggc cgatgccggc acctatggca tgggcatcat gagcgtgaaa
     6961 gacgcccgtg aggtgattgg cctgaaccgc aagcagcgca acaagatggc ggtgatcaag
     7021 gaaggccagg aagtcagcga ggtgatcgtg caagaaggcg tctacacctt cgagcaggtc
     7081 aacgaagccg tggccgagcc ggtggtgtat atgatcgacc gctatgtggt gggcggcttt
     7141 taccgggtgc acaccggccg tggcgtggac gaaaacctca acgcgccggg catgcacttt
     7201 gtgccgctgt cgtttgaatc gccctgcctg ccggaccgca agggcagccc ggattgcccg
     7261 cccaaccgct tttacgccta cggcgtggtg gcgcgcctgg ccttgctggc cggggcggtt
     7321 gagctggaca ataccgatcc cctgcgtggc tgatgttttc tggagaaccc ccgatgcgca
     7381 ttgcctttat tgccgacccg ctggagtcgt tcaagattta caaagactcc actttcgcca
     7441 tgatggaaga agccgcgcgc cgggggcatt cgctctacgc gctgctggcc gaggatatgt
     7501 acgtggccgc tggcgtggtg cgcgccaagg tcagcccgat cagcctgacc gggcacgaag
     7561 actggtttgt gcgcggcgcc gccgaagact gcgcgctgct ggcgtttgac gcggtggtga
     7621 tgcgcaaaga cccgccgttt gacgccgaat acctgtacgc cacccatctg ctgtcgctgg
     7681 cggaaaccca gggcgcgcgg gtgttcaatc gcggccaggc tttgcgtgac tataacgaga
     7741 agctggcgat tttgcagttt ccggaatgga ccacgcccac cctggtcagc tcgcagcccg
     7801 agcgcctgcg cgcctttgtc ggcgagcagg gcgacaccat cctgaaaccg ctggacagca
     7861 tgggcggcga cgggattttt cgcgtcagcc caagcgaccc caatctgtcg gtgattctgg
     7921 aaaccatgac cctgcgcggc gagcgcacca tcatggcgca gcgctatctg ccgcagattg
     7981 tcgatggcga caagcgcgtg ctggtgatcg acggcgagcc cgtgccctgg tgcctggcgc
     8041 gcatcccgaa agccggcgaa acgcgcggca atctggccgc tggcgggcgc ggcgaagcgc
     8101 gtgagctgag cgcgcgcgac cgtgaaatcg cccaggcgct gggcccgcgc ctgaaagccg
     8161 acgggctgtt gctggtgggg ctggacgtga ttggcgagca cctgaccgaa gtcaacgtca
     8221 ccagccccac ctgctttcgg gaaatcatgg accagaccgg cctgaacgtg gccggcctgt
     8281 tcatcgacgc gctggagcgg cgatgcgccg cctgagccgt ctggccggcc tgctggcggc
     8341 gctgatgctg ctggccggct gccagcgcga acccaccctg tggcagcaag aaagctatgt
     8401 gtttggcacc cgcgtgcaaa tcaccacggc ggatgtgcca gaaaacgtgg cgcgcccggc
     8461 ggtggccgcc gcgctggcgg atctggaccg ctggcatgtg cgcctgcacg cctggcgcga
     8521 cgacagcgag ctgacccgca tcaaccgcgc gctggccgcc ggccagccgg tggacaccga
     8581 cagcgaagtg ctgggcctgc tgcgccaggc gcaagatatc tcggcccgcg ccgagggcct
     8641 gtttaacccg gccatcggcc agttggtggc gctgtggggt tttcatgccg acagcttcgc
     8701 cccggtgcgc cccgagcccg gcgcgctggc ccggttgctc gccagcgcgc cgagcatggc
     8761 cgatgtgcag tttgacggcc agcgggtcag cagccgcaac cgcgcggtgt cgtttgacct
     8821 gggcggcatg gccaagggct gggcgctgga ccgggtggcc gaccggctgc gccaggcggg
     8881 tgttaagtct gccttaatca atatcggcgg taatatcctg gcgctgggca gcaagggcga
     8941 cacgccctgg aaggtgggcc tgcaagcccc gcgcgacaac caggcgatgg cggtgattgt
     9001 gttgcacgac ggcgaggcgc tgggcacctc gggcgattat cagcgctatt ttgagctgga
     9061 cggcgtgcgc tactgccatt tgatcgaccc acgcaatggc tcggccgcct gccagcgcca
     9121 ggccgccacc gtgctggtgg agccgggccc cgacgccggc ctgcgctcgg acgtggccag
     9181 caaaccgctg ttttttgccc cgcgcgccga catcgcccgg ctggccggcc gctttgccat
     9241 ccgcgatgtg ctgctggtgg aggccgacgg ccacacctgg ctgagcccgg cgatgcagcc
     9301 gcgcgtgcag tggctgcaac gcccgcagca ggtggatgtg ctgaccggct ttgctgaagc
     9361 gcccacctaa gcgccgctca caaaatcccc tggcgttgtt gtgtgacctt gccgtacttg
     9421 tgtactgtct gcgctcacac gcctagccag gggcgctgcg ctgagtgttg ggcgcggcgc
     9481 taaaccctga atccaacccc tctgtgacct cgccgccatg cggcggccac cccgcgagtt
     9541 ttaatgactg gacacactga aagcccgttc aagggcaaaa ccggcctgcg ccgcctgatc
     9601 aacgcgctcg gctattcgct ggatggcctg gctgcggcgt ttcgccacga ggcggcgttt
     9661 cgccagctgt cgctattggc cgccgtgctg ctgccgctgg ccatctggct gccggtgacg
     9721 ccgattgagc gcgcgctgat gatggcggcg ccgctggcca cgctgattat cgagctgttg
     9781 aattccgcca ttgaagccgc cgtggaccac acctcgctgg accgccaccc gctggccaaa
     9841 cgcgccaagg acatgggcag cgccgcccag ctgctgggcc tgatcaatct ggccgtggtg
     9901 tggggcggga ttttatatgg gcattatctg gcgtgatggc ggccggcgcc gtgggctgag
     9961 tggcgcttag cgcggctcac aaaacttagc gctgcgatcc tggctaagcg cccgaccgca
    10021 ggcagtacaa gttgtgcggc aaggtcaggc tacaacacca ggagcgtttt gtgcgccgct
    10081 cttagcgttg ccgcgcgggc gcttcgtcca gcgccacatc ttcgcgtttg caatacacca
    10141 cgccagggaa caccagaata tgctgctcgg tgagtgcgcc cacttgctcg cgctcgccga
    10201 caaattcaaa ccagcagcgg ctggaacgta aaaagcggta caccggccag ccggcgcgat
    10261 agaacatcag atcacgacag gtaaagtgat ccagcggcag caggctgtgg ctgcggcgcg
    10321 gctcaaacag catggacagc gcgtcagcgt cgccctcgcc ggacatcagc cgcgccaccc
    10381 gttcgctatc cgcctggcgc gcgccccaca gcaacactgc cagcgccagc ggcagcagca
    10441 gccaatcgcc gtccagcatg aacatcaccg acagcacccc cgcgccggtc agaaaaaacc
    10501 ggctatccca ttttgtcttt gttggcatga cgcaccccca tattgcctgg ccagcctgcg
    10561 ccggcgcgcg atgtattgtg tattgcacgt ttcagcctag atcaattcgc taaaaaatcc
    10621 ggaccaaaac agccgtgatg gccgagaatg tttttcggcc ttcctgaatt caggttaagc
    10681 aaaaatccgg cctattctac aggcggcgtt cgtgtttgcg gcaatggggg tgccatcatg
    10741 gcaattgcaa gctgttttga atgttttgct ttgtaaatca tgctattgga tattggcatc
    10801 gcccgctggc aattgaatgg gctgggcgct gggctatcca ccctctgtcc atgcataaaa
    10861 tatattgtcc gcggcctgac attctcagtt ggccaaggcg ggcgtgttcg gcgcggcgaa
    10921 cgggcgaacg gtgttttgtt cggtttcccg attactgttt gtgcgcatcg ggcaaaaaac
    10981 catatttgat caatctatta tcaaaaagtg cccgagggcg cgccgctggc gcgctttgtg
    11041 cgttattcgg gccacctgct cccacacccg acgggaggcg gccacggcgc gtcccaccca
    11101 gcgcgcccgt gcttcaaagc gtgaataaac tgcgaggaac tgtctgatga tgatccaaac
    11161 cctgcgtctg accgtattgt ccagcgtgct gctgtccgcc gccgtcatgg ccgccgacaa
    11221 gccgaacgtg atgattctgg ccactggcgg caccattgcc ggcaccggcg ccacctccac
    11281 caccaccgtc ggctacaccg ccgccaaggt gggcgtcgag cgcctgatcg aagctgtgcc
    11341 cgagctgaag aacgtcgcca acgtcaaggg cgagcaagtg ttccagatcg ccagcgaaaa
    11401 catgaccaac gaccactggc tgaaactgtc caagcgcgtc aacgagctgc tggcccagcg
    11461 tgacgtggac ggcatcgtca tcacccacgg caccgacacc ctggaagaaa ccgcct
//
LOCUS       DAOPYX010000058        11461 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_6438_length_11461_cov_10.898036, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000058 DAOPYX010000000
VERSION     DAOPYX010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 11461)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11461)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11461
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6438_length_11461_cov_10.898036"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..414
                     /gene="mraZ"
                     /locus_tag="PKN55_04585"
     CDS             <1..414
                     /gene="mraZ"
                     /locus_tag="PKN55_04585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011137898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HNX70552.1"
                     /translation="DSKGRLAIPARHRERLLAEFSGRLVVTLDSPKFLLVYPEKNWLP
                     VMEKLAALPVTHPGARAYARLLLGNAETLELDSAGRVLLPARLRQKAGLGKEVALVGM
                     GHKFELWNAAAFEAETDAALAMPAEALAELGALSL"
     gene            411..1364
                     /gene="rsmH"
                     /locus_tag="PKN55_04590"
     CDS             411..1364
                     /gene="rsmH"
                     /locus_tag="PKN55_04590"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011137897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HNX70553.1"
                     /translation="MSAETLIHRTVLLAEAVDALSVKPDGIYVDCTFGRGGHSRRILQ
                     QLGPQGRLIAFDKDPAAIANAQTIDDGRFQIVHDGFVCFAQALEALGVAQVDGVLMDL
                     GISSPQIDEAERGFSFRFDAPLDMRMDTTRGLTAAQWLAEADEADIREVIKTYGEERF
                     AKQIAAAIVASRAESPITRTGELARLVAQNVRTREPGQDPATRTFQAIRIYLNRELEE
                     LSATLPQIIGRLAQHGRLAVISFHSLEDRIVKHFLRDVSTADTLPSWAPVRAADIPEA
                     PVRLVGKAVRPSAEEVRDNPRARSAIMRVAERTAGVWKGQA"
     gene            1361..1645
                     /gene="ftsL"
                     /locus_tag="PKN55_04595"
     CDS             1361..1645
                     /gene="ftsL"
                     /locus_tag="PKN55_04595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014085614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HNX70554.1"
                     /translation="MRHKFTWFLLLALIVCALGVITSQHLARKLYSELEKEQKFTREL
                     EVEFGKLQLEQSTWAAHSLIERAATDRLSMHIPDPREVQVVQSSLAKRSQ"
     gene            1648..3396
                     /locus_tag="PKN55_04600"
     CDS             1648..3396
                     /locus_tag="PKN55_04600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008954697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HNX70555.1"
                     /translation="MAIVGATRHSTPSSSPSVRLPPSRMRLVGMGLGLLFVALLGRSI
                     YLQLVKQDFLQNQGAARYSRSIQLEAYRGVITDRNGEPLAISTPVQSIWASPADMEPV
                     PVDKINQLARLLEMDPGEVSRKLADKRREFLYLKRQISPELADAVMKLGIPGIAKQQE
                     YRRFYPAGEMMAHVVGFTGVDGRGQEGLELAKEKMLAGKPGSRHVIKDRRGHIIEDIA
                     AIERPRDGQTLSLSIDRKIQYLAFRELKTAVENFKARAGGIVVLDAHTGEVLALANLP
                     SFNPNNRQRLDPSMKRNRALTDLFEPGSTLKPFTVARALDLGIVRPDQVFNTESYSIG
                     PARIKDSHAASSLAVWEIIQKSSNVGSSKIALLMQPQTLWQMFDEVGFGRPPQSGFPG
                     EASGRVRPYKTWKPIEQATMSYGHGISLSLMQLARAYTIFTNDGELKPVSFMRLDQPL
                     PGKQVIRPDTARKMRDMLMTVTQPGGTAPRAQVLGFTVGGKTGTAHKQEGRGYAASKY
                     ISSFVGFAPATRPRLIVAVMIDEPQGQYYGGTVAGPVFSAVMSGSLRILGVEPDAPTN
                     NTLIPLDSIPEVREET"
     gene            3393..4877
                     /locus_tag="PKN55_04605"
     CDS             3393..4877
                     /locus_tag="PKN55_04605"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011137894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HNX70556.1"
                     /translation="MISQMTPLSVWEMSQLNALLAPAAQVVSDSRRVQPGDVFLAFRG
                     EYADGRDYIAAALERGAAAVLWDSADGFAWPAQWAATPNLAVPELRARAGVIASHALG
                     HPSQAMTVIGITGTNGKTSISHWLAQAFSLLDHKAALLGTVGNGFIGQLTAATHTTPD
                     PVTVQHKLAEYRAEGAQVVAMEVSSHGLDQFRVNGVAFATAVFTNLTRDHLDYHGDMA
                     AYGASKARLFHWQGLRHAVINVDDPFGRELVASLAGSGVQVFSYGLDGGNITVSQLEA
                     SLAGLRLSVRTPWGEGRIESPLLGRFNAANLLASLGALCANGVALADACAALGRIAPA
                     RGRMQHLGGGALPLVVVDYAHTPDALDKALATLADVRPAGGRLYAVFGCGGDRDPGKR
                     PQMGGIAQARADVAVVTSDNPRSEAPEAIIADILAGMSAPGHVDADRARAIDWAIAQA
                     QAGDVVLIAGKGHEEYQDIAGVKQPFSDFAVAEAALSRWSRQHG"
     gene            4870..6252
                     /gene="murF"
                     /locus_tag="PKN55_04610"
     CDS             4870..6252
                     /gene="murF"
                     /locus_tag="PKN55_04610"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018748369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HNX70557.1"
                     /translation="MADVMLTTYAAAQAIDGRASGADVGFARVTTDSRAVQAGDLFVA
                     LKGERFDAHDFVAGALAAGAAAALVRADFPELPGASLIRVDDTLAALGRLAAHWRTVG
                     FAGRMAGITGSSGKTSVKEMLRAILVAEAGEDAVLATAGNLNNDIGVPLTLLRLRGGH
                     RFAVIEMGMNHPGEIRYLASLARADAVAINNAQRAHAGHFASVADIARAKGELFESLP
                     AGVTACVNLDDAYAGLWQTLAGDARQIGFGWSQGEVRVTDCLLHSDRVALTLATPLGP
                     IAAEIPAPGEHVARNALTAVSLAIALGVSAAAIAQGLSQFAGAPGRLQRKLRADGVLV
                     LDDTYNANPDSMRAALRVLAAQPGARHAVLGDIGELGDGGPAMHAEVGAFARELGIDS
                     LHGLGELSAHTVAAFGSGGQHYADLDALLAGLAPLLHAPATVLVKGSRFMRMERVSAA
                     LLGQAPAAAH"
     gene            6370..7455
                     /gene="mraY"
                     /locus_tag="PKN55_04615"
     CDS             6370..7455
                     /gene="mraY"
                     /locus_tag="PKN55_04615"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008954700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HNX70558.1"
                     /translation="MLLLLANWLSEHIRTFNVLNYITLRAVLATLTSLGIGLYLGPWV
                     IRKLTELKVGQAVRDDGPQTHLIKAGTPTMGGTLILLSIGLTTLLWADLSNRFVWLLL
                     AVTGGTGLIGFVDDYRKVVYKNPKGLSARAKMFWQSAIAIAAGLFLSGTAHLPASTEF
                     IVPFFKNVVYPFGAIGFCVLTYFVIVGTSNAVNLTDGLDGLAALPVVLVSAALAVFAY
                     VAGHAVFAKYLGLPHIPGAHEVVVFCSAMCGACLAFLWFNAYPAQVFMGDVGALALGA
                     ALGTVAVIVRQEIVLFIMGGVFVMEALSVMIQVGSFKLTGKRVFRMAPLHHHYELKGW
                     KETQVVVRFWIITMMLVLAGLSTLKLR"
     gene            7470..8837
                     /gene="murD"
                     /locus_tag="PKN55_04620"
     CDS             7470..8837
                     /gene="murD"
                     /locus_tag="PKN55_04620"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018151926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HNX70559.1"
                     /translation="MKDLHGLTIGVVGLGDSGLSALRWLARRGARLVAIDSRTAPPHL
                     AELAQLAPEAELRAGPFSVDSFAGCQLLVVSPGVPLATPQIVAFAQAGGEVIGDVELF
                     ARAIAGQASKVIAITGSNGKTTVTSLVGHLCQHAGLNTVVAGNIGLPVLDALCDIEDS
                     GVWPEAFVLELSSFQLEGVRSLRADAATVLNISEDHLDRYQDLLDYAHSKTAIFAGDG
                     VQVLNQDDVFCRAMARPGRDIRRFALNAPAAYGLRRDGEAVWLAVDGEPVLDFARMQL
                     TGLHNAANALAALALCEAIGLPRAALLDGLCAFKGLAHRVEHVLQHQGVDFYDDSKGT
                     NVGATEAALNGMTRPVVLIAGGDGKGQDFSPLAPAVARIARAVLLIGRDAERIAAALA
                     GVSVPVQRVDTLEEATRLAYEAAQPGDVVLLSPACASLDMFRNYAHRAEVFISAARAL
                     AGEAA"
     gene            8834..10012
                     /gene="ftsW"
                     /locus_tag="PKN55_04625"
     CDS             8834..10012
                     /gene="ftsW"
                     /locus_tag="PKN55_04625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008954702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HNX70560.1"
                     /translation="MKFAGLLSREAAPRASVPLTDHDDALIWAVALLLSIGFVMVYSA
                     SIAYAEADPDTHNRHFYLIRHGVYLTISLIVGWVVYHIPSRVWQEKAPYLFVGAFVLL
                     ILVLVPGIGKVVNGSRRWINLVVLNLQPSELMKLAVVMYAANYTVRKAEWMHSVRKGF
                     MPMAIAVIATGALLLLEPDFGAFTVIASIAMGTLFLGGINGRIFAALLVVSLGAFVVL
                     IISSPYRLQRVTGFMDPWQDPYGKGYQLSHSLIAFGRGEWLGVGLGGSVEKLFYLPEA
                     HTDFLMAVIAEECGFIGIAVVIALFATVVRRAFVIAAQARDQERYFQALVAQGMGIWL
                     GVQSFINIGVNMGLLPTKGLTLPLLSYGGSALLANCSALAILLRIDWENRQTLRGYKV
                     "
     gene            10018..11085
                     /gene="murG"
                     /locus_tag="PKN55_04630"
     CDS             10018..11085
                     /gene="murG"
                     /locus_tag="PKN55_04630"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012698496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HNX70561.1"
                     /translation="MARRTALIMTGGTGGHIFPALAVADELKSRGWTLVFLGAEGGME
                     TKLVPQHGYTLETLPMRGMRGNGLRRWLSLPWMLARALAGAANVIFRHRPDVSVGFGG
                     YTGFPGGLMTRLLFKPLVIHEQNSVAGLTNRLLSRLASRTLFAFPAAFPQREGLVGNP
                     VRAAIAAVPAPEARFAGRSGPLRVLVVGGSLGAQALNEALPAALARLPATQRPQVTHQ
                     AGAKAIDALRAAYAAVKVNADCRSFIDDMASEYAQADLVICRAGALTVAELAAVGVGS
                     VLVPYPHAVDDHQTGNARYLADAGAARLWPQGELNAEALADWLAALTRERCLEMAQAA
                     RRMALTDAASKVADVCAELAP"
     gene            11184..>11461
                     /locus_tag="PKN55_04635"
     CDS             11184..>11461
                     /locus_tag="PKN55_04635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008954704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase domain-containing protein"
                     /protein_id="HNX70562.1"
                     /translation="MKHRVKHIHFVGIGGVGMSGIAEVLLTLGYAVSGSDMADSATTR
                     RLVNAGAKVYFGHDAGYIAGADVVVTSTAVKADNPEVLAAEAARIPVVP"
BASE COUNT         1804 a         3565 c         3949 g         2143 t
ORIGIN      
        1 gacagcaaag gtcgcctggc cattccggcc cggcaccgcg agcgcctgct ggccgaattc
       61 tccggtcgcc tggtggtgac cctggattcc ccgaagtttt tgcttgttta cccggaaaag
      121 aactggcttc ctgtgatgga aaaactcgcg gccctgcccg tgacacaccc tggcgcccgc
      181 gcctacgccc gcctgctgct gggcaatgct gaaaccctgg agctggattc tgccgggcgc
      241 gtgttgctgc ctgcccgttt gcgccaaaaa gccggactgg gcaaagaggt tgcgctggtg
      301 ggcatgggcc ataaattcga gctgtggaac gccgccgcct ttgaagcgga aaccgacgct
      361 gcgctggcca tgccggccga agcgctggct gaactggggg cgttgtcact gtgagcgccg
      421 aaaccctgat tcaccgcacg gtattgttgg ccgaagcggt tgatgccctg tcggtgaagc
      481 ccgacggcat ttatgtggat tgcaccttcg ggcgcggcgg tcacagccgg cgcatcctgc
      541 aacaactggg cccgcagggc cggctgattg cctttgacaa ggatccggcg gcgattgcca
      601 acgcccagac tatcgacgac ggccgttttc agattgtgca tgacggtttt gtctgttttg
      661 cccaggcgct ggaggcgctg ggcgtggcgc aggtggacgg cgtgctgatg gatttgggca
      721 tttcgtcgcc gcaaatcgac gaagccgaac gcggcttcag tttccgcttt gacgccccgc
      781 tggacatgcg catggacacc acccgtggcc tgacggcggc gcaatggctg gccgaggccg
      841 acgaggccga tatccgggaa gtgatcaaga cctatggcga agagcggttt gctaaacaga
      901 ttgcagcggc gattgtggcg tctcgggccg aatccccgat cacgcgcacg ggcgagctcg
      961 cccgcctggt cgcgcaaaac gtgcgtactc gtgaaccggg gcaggatccg gccacgcgca
     1021 cctttcaggc tattcggatt tacctcaatc gtgaactcga agaattaagc gccaccctgc
     1081 cgcaaatcat cggccggctg gcgcagcacg gccgcctggc ggtgatcagc tttcattcgc
     1141 tggaagaccg catcgttaaa cactttttgc gcgatgtgtc cactgccgac accctgccct
     1201 cctgggcgcc ggtgcgcgcg gccgacattc ccgaagcacc ggtgcgcctg gtgggcaagg
     1261 ccgtgcgccc cagcgcggaa gaagtgcgcg acaacccgcg tgcgcgcagc gccatcatgc
     1321 gcgtggctga gcgcaccgcc ggcgtctgga aaggccaggc atgagacaca agttcacctg
     1381 gtttctgctg ctggcgctga ttgtctgcgc gctgggggtg attacctcgc agcatctggc
     1441 gcgcaaactg tatagcgagc tggaaaaaga acagaaattc acccgtgaac tggaagtcga
     1501 gttcggcaag ctgcaactcg aacaaagcac ctgggcggcg cattcgctga ttgagcgcgc
     1561 cgccaccgac cggctgtcca tgcacattcc cgacccgcgc gaagtgcagg tggtgcaatc
     1621 cagcttagcc aagcggagtc aatgaccatg gccattgtcg gcgctacccg ccattccacc
     1681 ccgtcttcca gccccagcgt gcgcctgccg ccctcgcgca tgcgcctggt gggcatgggt
     1741 ttgggcctgc tgtttgttgc gctgctgggg cggtcgattt atttgcagtt ggtcaagcag
     1801 gatttcctgc aaaaccaggg ggctgcacgt tatagccgca gcattcagct ggaggcctat
     1861 cgcggggtga ttaccgaccg caatggcgag ccgctggcga tttccacccc ggtgcaatcc
     1921 atctgggcca gtccggcgga catggagccg gtgccggtgg acaaaatcaa ccaactggcc
     1981 cgcctgctgg aaatggaccc aggcgaagtc agccgcaagc tggccgacaa gcgccgtgaa
     2041 tttttgtatc tgaagcggca aatcagcccg gagctggctg acgcggtgat gaagctgggc
     2101 attcccggca tcgccaagca gcaggaatac cgccggtttt atccggccgg cgagatgatg
     2161 gcgcatgtgg tgggctttac cggcgtggat ggtcgcggcc aggaagggct ggagctggcc
     2221 aaggaaaaaa tgctggccgg caagccgggc agccgccatg tcatcaagga ccggcgcggc
     2281 catatcattg aagacatcgc cgccatcgag cgcccgcgcg acggccagac gctgtcgctg
     2341 tcgattgacc gcaagattca gtatctggcg ttccgcgagc tgaaaaccgc cgtcgaaaac
     2401 ttcaaggcgc gcgctggcgg catcgtggtg ctggacgccc acaccggcga agtgctggcg
     2461 ctggcaaatc tgccctcgtt caatcccaat aatcgccagc gactggatcc gtccatgaag
     2521 cgcaaccgtg cgctgaccga cttgttcgag ccgggctcca ccctcaagcc gtttaccgtg
     2581 gcgcgggcgc tggatttggg cattgttcgc ccggatcagg tgttcaacac cgagagctac
     2641 agcatcggcc cggcgcgcat caaggattcc cacgcggcgt cttcgctggc ggtgtgggaa
     2701 atcatccaga aatccagcaa cgtcggctcg tccaagatcg ccctgttgat gcagccgcaa
     2761 accctgtggc agatgtttga cgaagtgggc tttggccggc cgccgcaaag tggcttccct
     2821 ggtgaggcca gcggccgcgt gcgtccgtat aaaacctgga agccgattga acaggccacc
     2881 atgagttacg gccatggtat ttcactcagc ctgatgcagc tggcgcgcgc ctacaccatc
     2941 tttaccaatg acggcgaact caagccggtg agctttatgc gcctggatca gcccttgccc
     3001 ggcaagcagg tgatccgccc ggacaccgcg cgcaagatgc gcgacatgct gatgaccgtc
     3061 actcagcccg gcggcactgc gccgcgcgcc caggtgctgg gctttaccgt tggtggcaag
     3121 accggcacgg cgcacaagca ggaaggccgg ggttacgccg ccagcaagta tatttcgtcg
     3181 tttgtcggct ttgccccggc cacccggccg cgcctgatcg tggcggtgat gatcgacgag
     3241 ccgcaaggcc agtattacgg cggcacggtg gccggcccgg tgttcagcgc ggtgatgtcc
     3301 ggcagcctgc gcatcttggg cgtggagccg gatgcgccga ccaacaacac cttgattccg
     3361 ctcgacagca ttccggaagt gcgggaagaa acatgatcag ccagatgacc cctctctccg
     3421 tctgggagat gagccagctt aacgccttgc tggcccccgc cgcccaggtg gtgtccgaca
     3481 gccgccgcgt gcagcccggc gatgtgttcc tggcgtttcg cggcgaatac gccgacggcc
     3541 gcgactatat cgccgctgcg ctggagcgcg gcgccgccgc cgtgctgtgg gattcggccg
     3601 atggctttgc ctggccggcg cagtgggcgg ccacgcccaa tctggccgtg cccgagctgc
     3661 gcgcgcgcgc cggggtgatc gccagccatg cgctgggcca ccccagccag gcgatgacgg
     3721 tgattggcat caccggcacc aatggcaaaa catccatttc ccactggctg gcgcaggcgt
     3781 tttcgctatt ggatcacaag gccgcgctgc tgggcacggt aggcaatggc tttatcggcc
     3841 agctcaccgc cgccacccac accacgcccg acccggtgac cgtgcagcac aagctggccg
     3901 agtaccgcgc cgagggcgcg caggtggtgg cgatggaagt ctccagccac gggctggatc
     3961 agtttcgcgt caacggcgtg gcctttgcca cggcggtgtt caccaacctc acccgcgacc
     4021 atctggacta ccacggcgat atggccgcct acggcgccag caaggcgcgc ctgtttcact
     4081 ggcagggcct gcgccacgcg gtgatcaatg tggacgatcc ctttggccgc gagctggtcg
     4141 ccagcctggc cggcagcggc gtgcaggtgt tcagctacgg gctggacggt ggcaacatca
     4201 ccgtcagcca gctggaggcc tcgctggccg ggctgcggct gagcgtgcgc acgccatggg
     4261 gcgagggccg gattgaatcg ccgctgttgg gccgctttaa cgccgccaat ctgctggcca
     4321 gcctgggcgc gctgtgcgcc aacggcgtgg cgctggccga cgcctgcgcg gcgctgggcc
     4381 gcattgcccc ggcgcgcggg cgcatgcagc atctgggcgg cggcgcgttg cccctggtgg
     4441 tggtggacta cgcgcacacc ccggacgcac tggacaaggc gctggccacc ctggctgacg
     4501 tccgcccggc cggcggcagg ctctatgcgg tgtttggctg cggcggcgac cgtgaccccg
     4561 gcaagcgccc gcaaatgggc ggcattgccc aggcgcgcgc cgatgtggcg gtggtcacca
     4621 gcgacaaccc gcgcagcgaa gcgcccgagg ccatcatcgc cgatattctg gccggcatgt
     4681 ccgcgccggg ccatgtggat gctgaccgcg cccgcgccat cgactgggcg attgcccagg
     4741 cgcaggcggg cgatgtggtg ctgattgccg gcaagggcca cgaggaatac caggacattg
     4801 ccggcgtcaa gcagccgttt tctgattttg ccgtggccga ggccgcgcta agccgctgga
     4861 gccgccaaca tggctgatgt gatgctgacc acttacgccg ccgcccaggc gattgacggc
     4921 cgtgccagcg gcgcggacgt cggctttgcc cgcgtcacca ccgacagccg cgccgtgcag
     4981 gccggggatt tatttgtcgc tctcaagggc gagcgctttg acgcgcatga ttttgtcgcc
     5041 ggcgcgctgg ctgctggcgc ggcggcagcg ctggtgcgcg cggattttcc tgagctgccc
     5101 ggcgccagcc tgatccgggt ggacgacacc ctggcggcct tgggccggct ggcggcgcat
     5161 tggcgcacgg tgggctttgc cgggcgcatg gccggcatca ccggcagcag cggcaagacc
     5221 agcgtcaagg aaatgctgcg cgccatcctg gtggccgaag ccggcgaaga cgccgtgctg
     5281 gccaccgccg gcaatctgaa caacgacatc ggcgtgccgc tgaccctgct gcgcctgcgc
     5341 gggggacacc gttttgccgt gatcgagatg ggcatgaacc atcccggcga aatccgctat
     5401 ctggccagcc tggcgcgcgc cgatgcggtg gccatcaaca acgcccagcg cgcccacgcc
     5461 ggccattttg cctcggtggc ggacatcgcc cgcgccaagg gcgagctgtt tgaatccttg
     5521 cctgccggcg tcaccgcctg cgtgaacctg gacgacgcct acgccggcct gtggcaaacc
     5581 ctggctggtg acgcccgcca gattgggttt ggctggtcgc agggcgaggt gcgcgtcacc
     5641 gattgcctgt tgcacagcga ccgcgtggcg ctgaccctgg ccaccccgct ggggccgatt
     5701 gccgcagaga ttcccgcgcc gggcgagcat gtggcgcgca acgccctgac tgcggtcagc
     5761 ctggcgattg ccctgggcgt gtcggcggcg gcgattgccc aaggcctgag ccagtttgcc
     5821 ggcgcgcccg gccgcttgca gcgcaaactg cgcgccgacg gcgtgctggt gctggacgac
     5881 acctacaacg ccaacccgga ctccatgcgc gccgcgctgc gcgtgctggc ggcgcagccg
     5941 ggcgcgcgcc acgccgtgct gggcgatatc ggcgaattgg gcgatggcgg cccggccatg
     6001 cacgccgaag tgggcgcttt tgcccgcgaa ctgggcatcg acagcctgca tggcctgggc
     6061 gagctgagcg cacacacggt ggcggcgttt ggctcgggcg gccagcatta tgctgacctc
     6121 gatgcgctgc tggccgggct tgcgccgctg ttgcacgcgc cggcgacggt gctggtgaag
     6181 gggtcgcgct tcatgcgcat ggagcgggtc agcgccgcct tgctggggca agccccggcg
     6241 gcggcacact gagtttgacc gctgcgcggg ggcgcggcgg atgacacata taaaaaccag
     6301 gattgcatgg cgctgcccgc gcagtgtggc gggggcgcag caatccagat gatctgaggg
     6361 agctgcaacg tgttactgct gctggccaac tggctgagcg aacatatccg cacattcaat
     6421 gtgctgaact acatcacctt gcgcgcggtg ttggccacgc tgacttcgct gggtattggt
     6481 ctgtatctgg ggccgtgggt gattcgcaaa ctgaccgagc tgaaagtcgg ccaggcggtg
     6541 cgcgacgacg gcccgcaaac gcatttgatc aaggctggca cgccgaccat gggcggcacg
     6601 ctgattttgt tgtccatcgg cctgaccacg ctgctgtggg ctgacctgag caaccgtttt
     6661 gtctggctgc tgctggcggt gactggcggc acgggcttga tcggctttgt cgatgactac
     6721 cgcaaggtgg tgtacaaaaa cccgaaaggc ctgtcggcgc gcgccaagat gttctggcag
     6781 tcggccatcg ccattgccgc cggtttgttt ttgtctggca ccgcgcattt gccggcgtcc
     6841 accgagttta tcgtgccgtt cttcaagaac gtggtttacc cgtttggcgc gattggcttt
     6901 tgcgtgctga cttatttcgt gattgtcggc acctcgaatg ccgtcaacct gaccgacggc
     6961 ctggatggcc tggccgccct gccggtggtg ttggtcagcg cggcgctggc ggtgtttgcc
     7021 tatgtggccg gccacgcggt gttcgccaag tatctgggct tgccgcatat tcccggcgcg
     7081 cacgaagtgg tggtgttctg ctcggccatg tgcggcgctt gtctggcgtt tttgtggttc
     7141 aacgcctacc cggcgcaggt gtttatgggc gacgtcggcg cgctggccct gggcgcggcc
     7201 ctgggcacgg tggcggtgat tgtgcgtcag gaaatcgtgc tgttcatcat gggcggggtg
     7261 ttcgtgatgg aagcgctgtc ggtgatgatt caggtgggca gcttcaagct caccggcaag
     7321 cgggtgtttc gcatggcgcc gctgcatcac cactatgaat tgaagggctg gaaggaaacc
     7381 caggtggtgg tgcgcttctg gatcatcacc atgatgctgg tgctggctgg cctgtccacc
     7441 ctgaaactgc gataagagag caagaggcga tgaaggattt gcacggactc accatcggcg
     7501 tggttggcct gggcgacagc ggcttgtcgg cgctgcgctg gctggcgcgt cgcggcgcgc
     7561 ggctggtggc gattgacagc cgcacggcgc cgccgcatct ggccgagctg gcgcagctgg
     7621 cccccgaggc cgagttgcgc gccggcccct tctccgtcga cagctttgcc ggctgccagc
     7681 tgctggtggt cagtcctggc gtgcccttgg ccacgccgca aatcgtggcg tttgcccagg
     7741 ctggcggcga ggtgatcggc gatgtggagt tgtttgcccg cgccatcgcc gggcaggcca
     7801 gcaaggtgat cgccatcacc ggctcgaacg gcaagaccac ggtgacctcg ctggtggggc
     7861 atttgtgcca gcacgccggg ctgaacaccg tggtggccgg caatatcggc ctgccggtgc
     7921 tggatgcgct gtgcgacatc gaagacagcg gcgtctggcc ggaggcgttt gtgctggagt
     7981 tgtccagctt tcagctggaa ggcgtgcgca gcctgcgcgc cgacgccgcc acggtgctga
     8041 atatctctga agaccacctc gaccgctatc aggatttgct ggattacgcg cacagcaaga
     8101 cagcgatttt tgccggcgac ggcgtgcagg tgctcaatca ggacgatgtg ttctgccgcg
     8161 ccatggcgcg tccgggccgc gacatccgtc gctttgcgct gaatgcgccg gccgcctatg
     8221 gcctgcgccg cgatggcgag gcggtgtggc tggcggtgga cggtgagccg gtgctggatt
     8281 ttgcccgcat gcagctcacc ggcctgcaca acgccgccaa cgcgctggcc gcgctggcct
     8341 tgtgcgaggc gattggcctg ccgcgcgcgg cgctgctgga cggcctgtgc gcgttcaagg
     8401 ggctggcaca tcgggtggag catgtcttgc agcatcaggg cgtggacttt tacgacgatt
     8461 ccaaaggcac caatgtcggc gccaccgaag ccgcgctgaa tggcatgacc cggccggtgg
     8521 tgctgattgc cggcggcgac ggcaagggcc aggacttttc gcccctggcc ccggccgtgg
     8581 cgcgcattgc ccgcgcggtg ctgttgattg gccgcgacgc cgagcgcatc gccgcggccc
     8641 tggccggcgt gagtgtgcca gtgcagcggg tggacacgct ggaagaagcc acccgcctgg
     8701 cctatgaggc tgcgcagccg ggcgatgtgg tgctgttgtc gccggcctgc gccagcctgg
     8761 acatgttccg caactacgca catcgcgccg aggtgtttat ctctgccgca cgggccctgg
     8821 ccggggaggc ggcgtgaaat tcgctggctt attgagccgt gaggccgcgc cgcgcgccag
     8881 cgtgccgctg accgaccacg acgatgcgct gatctgggcg gtggccttat tgctgtcgat
     8941 tggttttgtc atggtgtatt cggcatcgat tgcctacgcc gaggccgacc cggacacgca
     9001 taaccgccat ttttacctga tccgccatgg cgtgtatctg accatctcgc tgattgtcgg
     9061 ctgggtggtg taccacatcc cgagccgggt ctggcaggag aaagcaccgt atctgtttgt
     9121 tggcgccttt gtgctgttga tcctggtgct ggtgcctggc atcggcaagg tggtgaacgg
     9181 ctcgcggcgc tggatcaatc tggtggtgct gaatttgcag ccatccgaac tgatgaagct
     9241 ggcggtggtg atgtatgccg ccaattacac cgtgcgcaag gccgagtgga tgcacagcgt
     9301 gcgcaaaggc tttatgccaa tggccattgc ggtgatcgcc accggcgccc tgctgctgct
     9361 ggagcccgac tttggcgcct tcaccgtgat tgcctcgatt gccatgggca cgttgtttct
     9421 gggcggcatc aacgggcgga tttttgccgc gctgctggtg gtcagtctgg gggcttttgt
     9481 ggtgctgatc atcagctcgc cctaccgctt gcaacgggtg accggcttta tggacccgtg
     9541 gcaagacccg tacggcaagg gctatcagtt gtcgcactcg ctgattgcct ttggccgggg
     9601 cgagtggctg ggcgtgggcc tgggcggcag cgtggaaaaa ctgttctacc tgccagaggc
     9661 gcatactgac tttttgatgg cggtgattgc cgaagaatgc ggctttatcg gtatcgccgt
     9721 ggtgattgcc ctgtttgcca ccgtggtgcg ccgcgccttt gtgattgccg cgcaggcgcg
     9781 cgaccaggag cgctattttc aggcgttggt ggcgcagggc atgggcatct ggctgggcgt
     9841 gcagtcgttt atcaatatcg gcgtgaacat gggcctgttg ccgaccaagg gcctgaccct
     9901 gccgctgctg tcctatggcg gcagtgcact gctggccaat tgttcggcgc tggcgattct
     9961 gctgcgcatt gactgggaaa accgccagac cctgcgcggc tacaaggtgt aagcaagatg
    10021 gcaagacgca ctgccctgat catgaccggc ggcaccggcg ggcatatttt cccggcgctg
    10081 gcggtggcgg acgaactcaa gtcgcgcggc tggacgctgg tgtttcttgg cgccgaaggc
    10141 ggcatggaaa ccaaactcgt gccgcagcac ggctatacgc tggaaaccct gccgatgcgc
    10201 ggcatgcgcg gcaatggcct gcgccgctgg ctgtcgctgc cgtggatgct ggcgcgcgcc
    10261 ttggccggcg cggccaatgt gatttttcgc caccggccgg atgtgtcggt ggggtttggt
    10321 ggctacaccg gctttcccgg cgggctgatg acccggctgt tgttcaagcc gctggtgatt
    10381 cacgagcaga actcggtggc cgggctgacc aaccggctgt tgtcgcggct ggcttcgcgc
    10441 accttgtttg cctttccggc ggcgtttccg cagcgcgagg ggctggtggg caatccggtg
    10501 cgcgccgcca tcgccgccgt gccggcgccc gaggcgcgtt ttgccggccg cagcggcccc
    10561 ttgcgcgtgc tggtggtggg cggcagtctg ggcgcgcagg cgctgaacga ggcgctgccg
    10621 gcggcgctgg cgcgcttgcc agcgacgcag cgcccgcagg tgacccacca ggccggcgcc
    10681 aaggcgatcg acgcgctgag ggccgcttac gccgccgtga aagtgaatgc cgactgccgc
    10741 agctttatcg acgacatggc cagcgaatac gcccaggcag atctggtgat ttgccgtgcc
    10801 ggtgcactga ctgtggccga gctggcggcg gtgggggtgg gcagtgtgct ggtgccgtat
    10861 ccgcacgcgg tggacgacca ccagaccggc aacgcccgtt atctggctga cgctggcgct
    10921 gcgcggctgt ggccgcaggg tgaactgaac gccgaagcct tggccgattg gctggcggcg
    10981 ctgacgcgag agcgctgtct ggagatggcg caggcggcgc ggcgcatggc tttgaccgac
    11041 gcggcaagca aggtagccga cgtgtgcgct gagctggcgc catgacaaac gatgtaaata
    11101 tgtactgcca ggcccgcttt gcggcacaat agcaaacttt ttgacgttgc cccggacgcg
    11161 gggcggctgg aacgcatcga gcgatgaaac atcgagtcaa gcatattcac tttgtcggca
    11221 tcggcggcgt cggcatgagc ggtatcgccg aggtcttgct gaccttgggt tacgccgtgt
    11281 ccggctcgga catggccgac agcgccacca cgcgccgtct ggtcaacgcc ggggcaaaag
    11341 tgtattttgg gcatgatgcc ggatatattg ccggcgcgga tgtggtggtg acatccacgg
    11401 cggtgaaagc cgacaacccg gaagtgctgg ctgccgaggc cgcgcgcatt ccggtggtgc
    11461 c
//
LOCUS       DAOPYX010000059        11357 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_6522_length_11357_cov_7.810122, whole genome shotgun sequence.
ACCESSION   DAOPYX010000059 DAOPYX010000000
VERSION     DAOPYX010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 11357)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11357)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11357
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6522_length_11357_cov_7.810122"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..498)
                     /locus_tag="PKN55_04640"
     CDS             complement(<1..498)
                     /locus_tag="PKN55_04640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012696459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HNX70563.1"
                     /translation="MQHDYLERILTSRVYDVAIETPLDPAPGLSRRLNNTILLKREDL
                     QPVFSFKLRGAYNKMAKLTPEQRQRGVIAASAGNHAQGVALSAQRLGCEATIVMPVTT
                     PQVKIDGVSRRGGKVVLHGDSYSDAYLHAKKLEAENGMTFIHPYDDPDVIAGQGTIGM
                     EILRQH"
     gene            complement(685..1512)
                     /locus_tag="PKN55_04645"
     CDS             complement(685..1512)
                     /locus_tag="PKN55_04645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012697788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNX70564.1"
                     /translation="MHSILITGCSSGIGHTVAHGLAREGWRVFASARAEADVARLRAE
                     GLSDALQLDVDDSASIERALAQVLAATGGTLDALFNNAGFGQPGAAEDISRAALRAQF
                     ETNLFGAWELTTRVLPVMRAQGHGRVLFNSSVLGFAAMRWRGAYNASKYAMEGLADTL
                     RLELAGSNVHVSLIEPGPITSRFRPNALAKFRAHVDWTHSVHTEVYRAQIERMEKPGP
                     AAPFTLPAEAVLDAVRRALNARRPAARYRVTFPTHLFWWLKRLLPTRWLDAVLTRAA"
     gene            1757..2707
                     /locus_tag="PKN55_04650"
     CDS             1757..2707
                     /locus_tag="PKN55_04650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009119159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HNX70565.1"
                     /translation="MYAGFFAAVLVMVLIDMFALNRAGNHKVSVREALGWSAVWFAIA
                     MLFNAWLWWHIRGDVGSGLDEAARQALANQKALEFMTGYLIEKSLAVDNIFVFLLLFG
                     FFKVPDIYQRRVLVYGVVGAIIMRAVMIFIGAALVAKFSWVLYLFGAFLVFTGLKMML
                     PEKEEQQDLTNNPVLKWLRRHLRVTDEFHEEKFFIRRNGILWATPLFLVLVMIELTDL
                     VFAVDSIPAIFSVTQDPFIVMTSNIFAILGLRALYFLLADVADRFHLLKYGLAAVLTF
                     IGMKMLLLDVFHMPVAVSLGVVFGLLTASIVASLLVTRKS"
     gene            complement(2905..3660)
                     /locus_tag="PKN55_04655"
     CDS             complement(2905..3660)
                     /locus_tag="PKN55_04655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012696685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HNX70566.1"
                     /translation="MDIGAFIIGDELLSGKRQDKHLPKLIELLAARGLKLAWAQYLGD
                     NPARLLAALRHSLASGDLVFSFGGIGATPDDHTRACAAAALDVPLAIHPEGRLLVEGR
                     FGDEAYPHRINMVNIPEGSRLIPNPVNQMPGFSVHDHHFVPGFPSMAWPMVEWVLDTQ
                     YRHLFAPGADIELTVMAEHAREGDLIDLMQQLVAEHPAARLSSLPSFGTDTLPPHIEF
                     GFTGQPDAARAAMAALTAGLQAKGYVLREVRQR"
     gene            3799..4269
                     /gene="nrdR"
                     /locus_tag="PKN55_04660"
     CDS             3799..4269
                     /gene="nrdR"
                     /locus_tag="PKN55_04660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012696216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HNX70567.1"
                     /translation="MKCPFCGHADTQVIDSRVSEDGASIRRRRRCLHCEKRFTTFETA
                     EVRMPQVVKQNGHRAEYDAGKVYTSFMRALHKRPVSTTLVDAAIERIGHKLLALGERE
                     ISSREIGEMVMNELAKLDKVAYVRFASVYRSFQDVSEFSDAIREISHQHSPPQS"
     gene            4266..>5347
                     /gene="ribD"
                     /locus_tag="PKN55_04665"
     CDS             4266..>5347
                     /gene="ribD"
                     /locus_tag="PKN55_04665"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003788782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HNX70568.1"
                     /translation="MTATFRPEDHAWMAQALRLAAQGLFTTTPNPRVGCVLVRDGQLV
                     GSGWHHRAGEPHAEVHALAEAGAQAAGATAYVTLEPCSHHGRTPPCADALIAAGVSRV
                     VAAMTDPNPLVAGRGLARLAAAGVIAEHGLLAAEAAELNKGFIQRMTQQRPWVTVKLG
                     ASLDGKSALSDGRSQWITGPAARADVQRLRARSCAVLTGAGTVMADNPRLTVRDFAVE
                     RQPLRVVAEGVMCAGALERAVYQDGGRTVVACALAHPGQRAKLQAQGVTVWELPLANN
                     PGKVDLPVLLAQLAQEYGVNELLVEAGMGVCGALLQADLVDELVIYQAPVTLGHDARG
                     LFDIPPLPNLDVARRWQWHDVAKVGN"
     assembly_gap    5348..5447
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(5539..6330)
                     /gene="fabI"
                     /locus_tag="PKN55_04670"
     CDS             complement(5539..6330)
                     /gene="fabI"
                     /locus_tag="PKN55_04670"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008954259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HNX70569.1"
                     /translation="MGFLQDKKILITGLLSNRSIAYGIAKACHREGAQLAFTYVNDKL
                     KDRVVDMAAEFGSDIVLRCDVANDEDINQLFVDLAQRWDGLDGFVHAIAFAPRESLEG
                     DFLDSLSREAFQVAHDISAYSFPALARAARPMMRGRNGACLTLSYLGAVRAIPHYNVM
                     GLAKASLEASVRFTAASLGRDNIRANGISAGPIKTLAASGISGFGKLLKMASDNSPLK
                     RNVTIEEVGNTAAFLLSDLSSGITGEITYVDAGYSINALNVPEEA"
     gene            6403..8208
                     /locus_tag="PKN55_04675"
     CDS             6403..8208
                     /locus_tag="PKN55_04675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009116206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HNX70570.1"
                     /translation="MWRQLLASFLWCFCLLAAWPAQAAPAMALGETPRYAPGFSHFDY
                     VNPNAPKGGRLLLPAMGSFDSLNPFTLKGDKESGLGLLVDTLMEKSLDEPFAMYGLLA
                     DDMALADDCLSVRFHLNPAARFSDGSPVLAEDVKASFDTLTRDKTAHPRYRMYFADVK
                     QVTVLDTRTVRFDFHRRNAELHMILGELPVFARRWLQGQPLASRTLQPPLASGPYLLA
                     RYALGKYSVYQRRPDYWARELPSRRGQYNFDRIEFRYMLDDTVRLEAFKAGEFDVSVE
                     NVAKQWARGYSGRAFASGALKKLEVPHGNGAGMQGFAMNLRRPLFADKRVRQALVLAF
                     DFDWANRQLFYRQYTRSDSYFSNSELAARGLPGADELALLNPLKDKLDPAVFGPAIDA
                     PKSPDALSLRANLRQAQKLLADAGWRMGDDGWLVNAAGQRLEFEFLTYTRTFERVAAP
                     YQRNLEKLGVKLNVRMVDPALFQKRLEQFDFDMTVAGFPQSASPGNEQLDYFGSAAAK
                     QPGSNNLIGLQDPAVDALLQRFVTFRHRAELVAASRALDRVLRAGYYLMPNWHLAYHR
                     IAYRDIFGRPERLPRYFQAQDWVERTWWAKPGARP"
     gene            8211..9263
                     /locus_tag="PKN55_04680"
     CDS             8211..9263
                     /locus_tag="PKN55_04680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019959372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNX70571.1"
                     /translation="MWRYLAKRLLLMIPTLLGVLLLTFAVIQFVPGGPVEQLTQQLRH
                     SAEGGEAASTASSGLYRSQGLDQEQLAELKALYGFDKPASQRFIDMLWRYARFDLGES
                     FYHHQSVGSLILSKLPVSMSLGLSSFLLTYLCCIPLGIAKAVRHGSTFDLLSSTVILV
                     GYAIPGFVLGVALLVLFGGGSFLQWFPLRGLTSDDWATLSPLGKALDYLWHLVLPVTA
                     SVVGSLAVMTLLTKNTFLEQIRCQYVLTARAKGLSERKILYKHVFRNAMIPLVTGFPA
                     AFIGAFFAGSLLIETLFSLDGLGLLSYESVVKRDYPVVMGSLYVFTLVGLFTRLLSDL
                     CYVWVDPRVQFDAARG"
     gene            9285..10373
                     /locus_tag="PKN55_04685"
     CDS             9285..10373
                     /locus_tag="PKN55_04685"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 family protein"
                     /protein_id="HNX70572.1"
                     /translation="MSSTPFEQLLENARHNELVMSKMQALEMALISAGDFPQLLGLLL
                     DDIQSEFALDSVHLHWVDPQGEVQALLCGGGLDLDEYPRLHFIPSQFPLTHLLNPGLR
                     TRLCAFQPGQHGDWLPDEGMGSLALLPLVRQGHLLGCLVLGSRDPQRFDPTLSTDFLE
                     RLAAIAGVCLENVYHHEQIKRLGLTDALTGVHNRRYFDQRLDEEVTRAQRQGSCLGGL
                     FLDIDFFKRINDTWGHPAGDAVLQEVARRVKSMLRITDSLARYGGEEFAVLLAQTDLE
                     QAARVAERIRAAVAAGPVCLGGGLDIPVTLSIGASCLHGLLPQETPGEAARRLLHEAD
                     SALYRAKENGRNQVMCHTAEPAGAPDHG"
     gene            10366..>11357
                     /locus_tag="PKN55_04690"
     CDS             10366..>11357
                     /locus_tag="PKN55_04690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012696581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNX70573.1"
                     /translation="MADAAILTPRQRAWRRFAGNRRGWWSLWIFLALFGLSLFAELLS
                     NDKPIVAHYQGEYYFPLWHTYPETTFGGDFRSPTDYLDPFIRQQLSRDGNWALYPPNP
                     YRFDTLNYFAQAANPAPPSADNLLGTDDRGRDVLARLLYGFRVSVLFALALTAIGSLL
                     GILAGAVQGYFGGRTDLILQRLIEIWGSLPELYLLIIFASLFTPSLWLLLALLSLFGW
                     MGLSDYVRAEFLRNRQMEYVLAARALGLSNSAIMWRHILPNSLTPVITFLPFRISGAI
                     LALTSLDFLGLGVPASTPSLGELLAQGKDNLDAWWISLSTFGVLVGTLVLLIFIG"
BASE COUNT         1912 a         3547 c         3646 g         2152 t
ORIGIN      
        1 gtgctggcgc aggatttcca tgccgatggt gccctggccg gcgatcacgt ccgggtcgtc
       61 atacggatgg ataaaggtca tgccgttttc ggcttccagc tttttggcgt gcaggtaggc
      121 gtcgctgtac gaatcgccat gcagcaccac cttgccgccg cgccgcgaca cgccgtcgat
      181 tttcacctgc ggggtggtca ccggcatgac gatggtggct tcgcagccca gccgttgcgc
      241 cgacagcgcc acgccctggg cgtggttgcc ggccgaggcg gcaatcaccc cgcgctggcg
      301 ttgctcgggg gtgagcttgg ccattttgtt gtaggcgccg cgcagcttga acgagaacac
      361 cggctgcaag tcttcgcgct tgagcaaaat ggtgttgttc aagcggcgcg acaggccagg
      421 ggctgggtcc agcggggttt cgatggccac gtcgtagacg cgggaggtga ggatgcgttc
      481 gaggtagtcg tgttgcatgt cggcagtcag ggcaggcgtc gcatcaaagc ggcaaagtca
      541 gcaatgacgt caagattagc aggaaagtgc ggcgacgggc agtgccgcag tgcaacaaaa
      601 tgccgcactg ccggtggcgt gtggtgcagt gggggcggct gacttgctca ggctgccgga
      661 cgatggacag cgagccggca agggttacgc cgcccgcgtc agcaccgcat ccagccagcg
      721 ggtgggcagc aggcgcttga gccaccagaa cagatgggtc ggaaaagtca cccgatagcg
      781 ggcggccggg cggcgggcgt tgagcgcgcg gcgaacggcg tccagcaccg cctcggccgg
      841 cagggtgaac ggcgcggccg ggccgggttt ttccatgcgt tcgatctgcg cgcggtacac
      901 ctcggtgtgc acgctgtgcg tccagtccac atgggcgcgg aatttggcca gggcgttggg
      961 ccgaaagcgg ctggtgatcg ggccgggctc aatcaggctg acatggacat tgctgccggc
     1021 cagctccagc cgcagggtgt cggccaagcc ttccatggcg tatttgctgg cgttgtaggc
     1081 gccgcgccag cgcatggcgg caaagcccag cacggaagaa ttaaacagca cgcggccgtg
     1141 gccttgtgcg cgcatcaccg gcagcacgcg ggtggtgagc tcccaggcgc caaacagatt
     1201 ggtttcaaac tgggcgcgca gcgccgcgcg cgagatgtct tcggcggcgc cgggctggcc
     1261 aaagccggcg ttgttgaaca gcgcatccag cgtgccgccg gtggcggcca gcacctgggc
     1321 cagcgcgcgc tcgatcgagg ccgagtcatc cacatccagt tgcagcgcgt cgctgaggcc
     1381 ctcggcgcgc agccgcgcca catcggcttc ggcgcgggcg ctggcgaaca cccgccagcc
     1441 ttcacgcgcc aggccgtggg cgacggtgtg gccaatgccg gaagaacaac cggtgatcaa
     1501 gatgctgtgc atgagtgcta taccttgtgg tgatgccaag ctgccgcatg gcggtgagca
     1561 cgcatgcgcc agccctggat tttttcggga acctctgcct gttgcggccg gtcatgcatg
     1621 ccgtgatgtc aaacgcggcg ggcagaatgt agggcaaagc cagcgattgt gccggttttt
     1681 cccgcagttt tgccggtttt taccatacag gagatcacat ttggaaactg gactgagttc
     1741 cgtaggcagc ccactgatgt acgccggctt ttttgccgcc gtgctggtga tggtgttgat
     1801 tgatatgttt gcgctcaacc gcgccggcaa tcacaaggtc agcgtgcgcg aggcgctggg
     1861 ctggtcggcg gtgtggtttg ccattgccat gctgttcaac gcctggctgt ggtggcacat
     1921 tcgcggcgac gtcggctccg gcctggacga ggccgcccgc caggcgctgg ccaatcagaa
     1981 agcgctggaa ttcatgaccg gctatctgat tgaaaaatcg ctggcggtgg acaatatttt
     2041 tgtgttcctg ctgctgttcg ggtttttcaa ggtgccggat atttatcaac gccgggtgct
     2101 ggtgtatggc gtggtcggcg ccatcatcat gcgggcggtg atgattttca tcggcgcggc
     2161 attggtggcc aagttctctt gggtgctgta tctgttcggc gccttcctgg tgttcaccgg
     2221 cctgaaaatg atgttgccgg aaaaagaaga acaacaggat ctgaccaata acccggtgct
     2281 gaaatggctg cgtcgccacc tgcgggtgac ggatgaattc cacgaagaaa aattctttat
     2341 tcgtcgcaat ggcatcttgt gggccacgcc gctgtttttg gtgctggtga tgattgagct
     2401 gaccgacctg gtgtttgccg tggacagcat cccggctatc ttctcggtga ctcaggatcc
     2461 gttcatcgtc atgacctcga acatctttgc cattctgggt ctgcgcgcgc tgtacttcct
     2521 gctggccgat gtggcggacc gtttccacct gctgaaatac ggcctggccg ctgtgctgac
     2581 ctttattggc atgaagatgc tgctgctgga cgtgttccac atgccggtgg cggtgtcgct
     2641 gggcgtggtg tttggcctgc tgaccgcgtc gattgtcgcc tcgctgctgg tcacgcgtaa
     2701 aagctgagcg gtctgcccgc tgagcagtgc gccagcatcg cgccgcccca tctccgggcg
     2761 gcgcgatgca tttgtgtctc agggctgggc ggtgtctggc tgcgcgtaga gtcgctcaca
     2821 aaacccagcg aagcggccct ggccaggcgt gcgggtagca cggcaaggtc acaccacaac
     2881 gccagggggt tttgtgagca acgcttaacg ctggcgcacc tcgcgcagta cataaccctt
     2941 ggcctgcaaa ccggcggtca gcgccgccat ggcagcgcgc gcggcgtcgg gctggccggt
     3001 gaagccaaac tcgatatgcg gcggcagagt gtcggtgcca aagctgggca ggctggaaag
     3061 ccgggcggcc gggtgttcgg ccaccagctg ctgcatcagg tcgatcaaat cgccttcgcg
     3121 ggcgtgttcg gccatcaccg tcagctcaat atcggcgcca ggggcgaaca aatggcggta
     3181 ttgcgtgtcc agcacccatt cgaccatcgg ccaggccatg ctgggaaagc caggcacaaa
     3241 gtggtgatcg tgcacgctaa agcccggcat ctggttcacc gggttgggaa tcagccggct
     3301 gccctccggg atgttcacca tattgatgcg gtgcgggtag gcttcgtcgc caaagcggcc
     3361 ttccaccagc agccggcctt ccgggtggat ggccagcggc acgtccagcg cggcggcggc
     3421 gcaggcgcgg gtgtggtcgt ccggcgtggc gccaatgccg ccaaagctga acaccagatc
     3481 gccgctggcc aggctgtggc gcagcgccgc cagcaagcgc gccgggttgt cgcccagata
     3541 ctgcgcccag gccagcttga gcccacgcgc ggccagcagt tcgatgagtt tgggcaggtg
     3601 tttgtcctga cgtttgcccg agagcagttc gtcaccaatg atgaaagcgc caatgtccat
     3661 gaggagttcg atcgtgagtg agtcaaaaaa cccgaatggc aaaaggataa tccgccgggc
     3721 gtccgggtgc aaaagcgcat ggcggcgcag gcccggtttc gataagatac gcgccatccg
     3781 ttttggggaa tccgtttcat gaaatgcccg ttttgcggcc atgccgatac gcaggtgatc
     3841 gattcgcggg tgagcgaaga cggcgccagc atccgccgcc gccgccgctg cctgcactgc
     3901 gaaaagcgct tcaccacgtt tgaaaccgct gaagtgcgca tgccgcaagt ggtcaagcag
     3961 aacggccacc gcgccgaata cgacgccggc aaggtgtaca ccagctttat gcgtgcgctg
     4021 cacaagcgcc cggtgtccac caccctggtg gacgccgcca tcgagcgcat cggccacaag
     4081 ctgctggcgc tgggcgagcg ggaaatcagc tcgcgcgaaa tcggcgaaat ggtgatgaac
     4141 gaactggcca agctggacaa agtggcttat gtgcgttttg cctcggtata ccgcagcttt
     4201 caggacgtct cggaattctc tgacgccatt cgcgaaatct cccaccaaca cagcccgcca
     4261 caatcatgac cgccaccttc cgccccgaag accacgcctg gatggcgcag gccctgcgcc
     4321 tggccgccca gggcctcttc accaccacgc ccaatccgcg cgtgggctgc gtgctggtgc
     4381 gcgatggcca attggtgggc agcggctggc accaccgcgc cggtgagccg cacgccgaag
     4441 tgcacgcgct ggccgaagcc ggcgcccagg ccgccggcgc caccgcctat gtgacgctgg
     4501 agccatgcag ccaccacggc cgcaccccgc cgtgcgccga cgcgctgatt gccgctggcg
     4561 tcagccgcgt ggtggcggcg atgaccgacc ccaatccgct ggtggctggg cgcggcctgg
     4621 cgcggctggc ggcggcgggc gtgattgccg agcatggcct gctggccgcc gaggccgccg
     4681 agctgaacaa gggcttcatc cagcgcatga cgcaacaacg gccgtgggtg acggtcaagc
     4741 tgggggccag cctggacggc aaaagcgcgc tcagcgacgg ccgcagccag tggatcaccg
     4801 gcccggcagc acgcgccgat gtgcagcgcc tgcgcgcgcg cagctgcgcg gtgctgaccg
     4861 gcgccggcac ggtgatggcc gacaacccgc ggctgacggt gcgcgatttt gccgttgagc
     4921 gccagccgct gcgcgtggtg gccgaagggg tgatgtgcgc tggcgcgctg gagcgcgcgg
     4981 tgtatcagga tggcgggcgc accgtggtgg cctgcgcctt ggctcacccc ggccagcgcg
     5041 ccaagctgca agcccagggc gtgacggtgt gggagctgcc gctggccaat aaccccggca
     5101 aggtggattt gccagtgctg ctggcccagt tggcgcaaga atacggcgtg aatgaattgc
     5161 tggtggaagc cggcatgggc gtgtgcggcg ccttgttgca ggcggatttg gtggacgagc
     5221 tggtgattta tcaagcgccg gtcaccctgg gccacgacgc gcgcggcttg ttcgacatcc
     5281 cgccgctgcc caatctggac gtcgcccgcc gctggcagtg gcatgatgtg gccaaggtgg
     5341 gcaatgannn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnncga cggcccatct
     5461 tttcatccgc cgcccagatc gggagtggcc accgcacagc gtacggcagc acacccagcc
     5521 ggtaggggca gccggttttt acgcttcttc cggcacgttc agcgcattga tgctgtaacc
     5581 ggcatccaca taggtgattt cgcccgtgat gcccgaagac agatcagaca gcaagaaggc
     5641 ggcggtgttg ccgacttctt caatggtgac attgcgcttg agcggcgagt tgtccgaggc
     5701 cattttcagc agcttgccaa acccggaaat gcccgaggca gccagggttt tgatcgggcc
     5761 ggcggaaatg ccgttggcgc ggatattgtc gcggcccagg ctggcggcgg taaagcgcac
     5821 gctggcttcc aggctggcct tggccaggcc catcacattg taatgcggga tggcgcgcac
     5881 cgcgccaagg taggacagcg tcaggcaggc gccgttgcgg ccgcgcatca tcgggcgggc
     5941 ggcgcgggcc agggccggga agctgtaagc gctgatgtcg tgcgccacct ggaaagcctc
     6001 acgcgagagc gaatccagaa aatccccttc cagcgattct cgcggggcaa aggcaatggc
     6061 gtgcacaaag ccatccaggc cgtcccagcg ctgggccaga tcgacaaaca gctgattgat
     6121 gtcctcatcg ttggccacgt cgcaacgcaa gacgatgtcg ctgccgaatt cggccgccat
     6181 gtccaccacg cgatccttga gcttgtcgtt cacataggtg aaggccaact gggcgccttc
     6241 acggtgacag gctttggcga tgccataggc aatcgagcgg ttggaaagca ggccggtaat
     6301 caggatcttt ttgtcttgca gaaaacccat cgtcagtcct tggtaagtcg ggaaaccgag
     6361 cgattatagc ctaagccggg gcggcgcatt tacaatgcct gcatgtggcg acaacttctt
     6421 gcttcttttt tgtggtgctt ttgtctgctg gcggcctggc ccgcccaggc ggcgccggcc
     6481 atggcgctgg gtgaaacccc gcgctatgcg ccaggcttta gccattttga ctacgtcaat
     6541 cccaacgccc ccaagggcgg gcggctgctg ctgccggcca tgggcagctt tgattcgctc
     6601 aatcccttca ccctgaaggg cgacaaagaa tccggcctgg ggctcttggt ggacacgctg
     6661 atggaaaaaa gcctggacga gccctttgcc atgtatggcc tgctggccga cgacatggcc
     6721 ctggccgacg attgcctgtc ggtgcgcttt cacctcaacc cggccgcgcg ctttagcgat
     6781 ggctcaccgg tgctggccga agacgtcaag gccagctttg acaccctcac ccgcgacaaa
     6841 accgcccacc cgcgctaccg catgtatttt gccgacgtga aacaagtcac cgtgctggac
     6901 acacgcacgg tgcgctttga ctttcaccgc cgcaatgccg agctgcacat gattctgggc
     6961 gagctgccgg tgtttgcccg ccgctggctg caaggccagc cgctggccag ccgcaccttg
     7021 caaccgccgc tggccagcgg gccgtatctc ttggcgcgct acgcgctggg caaatacagc
     7081 gtgtatcagc gccggccgga ttattgggcg cgcgagctgc ccagccggcg cggccagtat
     7141 aattttgacc gcattgaatt tcgctacatg ctggacgaca ccgtgcggct ggaggcgttc
     7201 aaggctggcg aatttgacgt ctcggtagaa aacgtcgcca agcaatgggc gcgcggctac
     7261 agcggccgcg cctttgccag cggcgccctg aaaaaactgg aagtcccgca cggcaatggc
     7321 gccggcatgc agggctttgc catgaatctg cgccggccct tgtttgccga caagcgcgtg
     7381 cgccaggcgc tggtgctggc ctttgacttt gactgggcca accgccagct gttttaccgc
     7441 cagtacaccc gcagcgacag ttatttcagc aatagcgagc tggccgcgcg cggcctgccc
     7501 ggcgccgacg agctggcctt gctcaacccg ctgaaagaca agctcgaccc cgccgtattc
     7561 ggcccggcca tcgacgcgcc caagagcccg gatgcgctca gcctgcgcgc caatctgcgc
     7621 caggcgcaaa aactgctggc cgacgccggc tggcgcatgg gcgacgacgg ctggctggtc
     7681 aacgccgccg gccagcggct ggaattcgag tttctcacct acacccgcac ctttgagcgg
     7741 gtggcggcgc cgtatcagcg caatctggag aaactcggcg tcaagctgaa tgtgcgcatg
     7801 gtggacccgg cgctgttcca gaaacggctg gagcagtttg atttcgacat gacggtggcg
     7861 gggtttccgc aatcggccag ccccggcaat gaacagctgg attattttgg ctcggccgcc
     7921 gccaagcagc ccggctcgaa taatctgatc ggcctgcaag acccggcggt ggacgcgctg
     7981 ttgcagcgct ttgtcacctt tcgccaccgt gccgagctgg tggccgccag ccgcgcgctg
     8041 gaccgcgtgc tgcgcgccgg ctattacctg atgcccaatt ggcacctggc ctaccaccgc
     8101 attgcctacc gtgacatctt tggccgcccc gagcgcctgc cgcgctattt tcaggcgcag
     8161 gactgggtag agcgcacctg gtgggccaaa ccgggagccc gaccatgaat ctgtggcgct
     8221 atctggccaa acggctgttg ctgatgatcc ccaccctgct gggcgtgctg ctgctgacct
     8281 ttgccgtcat ccagtttgtg cccggcggcc cggtggagca gctcacccag caactgcgcc
     8341 acagcgccga gggcggcgaa gcggccagca ccgccagcag cgggctatac cgcagccagg
     8401 ggctggacca agagcaactg gccgaactca aagccctgta cggctttgac aaaccggcca
     8461 gccagcgctt tatcgacatg ctctggcgct acgcccgttt tgatctgggc gagagttttt
     8521 atcatcacca atccgtcggc agcctgattc tgtccaaact gccggtgtcg atgagcctgg
     8581 ggctttccag ctttttgctg acctatctgt gctgcattcc gctgggcatc gccaaggcgg
     8641 tgcgccatgg ctccaccttt gatttgctga gcagcacggt gattctggtg ggctacgcca
     8701 tccccggctt tgtgctgggc gtggcgctgc tggtgctgtt tggtggcggc agctttttgc
     8761 agtggtttcc gctgcgcggg ctgaccagcg acgactgggc tacgctgtcg ccgctgggca
     8821 aggcgctgga ttatctgtgg cacttggtgc tgccggtcac cgcctcggtg gtgggcagcc
     8881 tggcggtgat gacgctgctg accaaaaaca cctttctgga acaaatccgc tgccaatatg
     8941 tgctgaccgc ccgcgccaag ggtttgtccg agcgcaagat tttgtacaag catgtgtttc
     9001 gcaacgccat gatcccgctg gtgaccggct ttccggcggc gtttatcggc gcgttttttg
     9061 ccggcagcct gctgatcgaa accctgttct cgctggatgg cctgggcctg ttgtcgtatg
     9121 aatcggtggt caagcgcgat tacccggtgg tgatgggcag cctgtatgtg tttaccctgg
     9181 tggggctgtt tacccgcctg ttgtctgatt tgtgttatgt ctgggttgat ccgcgcgtgc
     9241 aatttgacgc cgcgcgcggc tgacccggca agaaagcgtg agccgtgtct tccactccgt
     9301 ttgaacaatt gctggaaaac gcccgccaca acgagctggt gatgtccaag atgcaagccc
     9361 tggaaatggc gctgatcagc gccggggatt tcccccaact gctgggcttg ctgctggacg
     9421 acatccagag tgaatttgcc ctggacagcg tgcacctgca ctgggtagac ccgcaaggcg
     9481 aggtgcaagc cctgctgtgc ggcggtgggc tggacctgga cgaatacccg cgcctgcact
     9541 tcattcccag ccaatttccg ctgacccatc tgctgaaccc cggcctgcgc acccgtttgt
     9601 gcgcctttca gccggggcaa catggcgact ggctgcccga cgaaggcatg ggcagcctgg
     9661 ccttgctgcc gctggtgcgc cagggccatc tgctgggctg tctggtgctg ggcagccgcg
     9721 acccccaacg ctttgacccc accttgtcca ctgattttct ggaacggctg gccgccattg
     9781 ccggcgtctg cctggaaaac gtctatcacc acgaacaaat caagcgcctg ggcctgaccg
     9841 acgcgctgac cggcgtgcat aaccgccgtt acttcgacca acgcctggac gaagaagtca
     9901 cccgcgccca acgccagggc agctgcctgg gcggactgtt tctggacatt gattttttca
     9961 agcgcatcaa cgacacctgg ggccatccgg cgggcgacgc cgtgctgcaa gaagtggccc
    10021 gtcgggtaaa aagcatgctg cgcatcaccg acagcctggc gcgctacggc ggcgaagaat
    10081 ttgccgtgct gctggcgcaa accgacctgg aacaagccgc gcgcgtggcc gagcgcatcc
    10141 gcgccgccgt ggccgccggc ccggtctgcc tgggcggcgg gctggatatc cccgtcacct
    10201 tatccatcgg cgccagctgc ctgcacggcc tgctgcccca ggaaacccca ggcgaagccg
    10261 cccgccgcct gctgcacgaa gccgacagcg cgctctaccg cgccaaagaa aacggccgca
    10321 atcaggtgat gtgccacacc gccgagcccg ccggagcgcc cgaccatggc tgacgccgcc
    10381 atcctcaccc cgcgccaacg cgcctggcgc cgctttgccg gcaaccggcg cggctggtgg
    10441 agcttgtgga tttttctggc gctgtttggc ctgagcctgt ttgccgagct attgagcaac
    10501 gacaaaccca tcgtcgccca ctaccagggc gagtattatt ttccgctgtg gcacacctac
    10561 ccggaaacca cctttggcgg cgattttcgc agccccaccg actacctcga cccctttatc
    10621 cgccagcaac tcagccgcga cggcaactgg gcgctctacc cgcccaaccc ctaccgcttt
    10681 gacacgctga actactttgc ccaggccgcc aacccggcgc cgccctcggc tgacaacctg
    10741 ctgggcaccg acgaccgcgg ccgcgatgtg ctggcgcgcc tgttatacgg ttttagagtg
    10801 tcggtgctgt ttgccctggc gctgaccgcc atcggctcgc tgctgggcat cctggccggc
    10861 gcggtgcaag gctattttgg cgggcgcacc gatttgattt tgcaaaggct gatcgaaatc
    10921 tggggctcgc tgcccgagct gtatctcttg atcatctttg cctcgctatt cacccccagc
    10981 ctgtggctgc tgctggcctt gctgtcgctg tttggctgga tgggcctatc cgactatgtg
    11041 cgcgccgaat ttctgcgcaa ccggcaaatg gaatacgtgc tggccgcccg cgcgctgggc
    11101 ctgtccaaca gcgccatcat gtggcggcac atcctgccca acagcctcac cccggtgatc
    11161 acctttttgc ccttccgcat ctccggcgcc atcctggcgc tgaccagcct ggactttctc
    11221 ggcctgggcg tgcccgcctc cacccccagc ctgggcgaac tgctggcgca aggcaaagac
    11281 aacctcgacg cctggtggat ttcgctatcc acctttggcg tgctggttgg cacgctggtg
    11341 ctgctgattt ttattgg
//
LOCUS       DAOPYX010000060        11287 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_6577_length_11287_cov_10.823629, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000060 DAOPYX010000000
VERSION     DAOPYX010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 11287)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11287)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11287
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6577_length_11287_cov_10.823629"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            30..1541
                     /locus_tag="PKN55_04695"
     CDS             30..1541
                     /locus_tag="PKN55_04695"
                     /inference="COORDINATES: protein motif:HMM:NF012573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX70574.1"
                     /translation="MTTSISASTNYTLGAGEDNLLLTGSADLKGTGNHANNQLEGNDG
                     ANVLIGGLGYDQLYAGAGDDVLIGNQAGVMQDNDADALFGHQGNDTLYLNGLDYADGD
                     EGADTYWVTGGSNSIQDNGPDAGVDVVKAFTSVSMPSYYAVQDPRYGNVSAYGIERVE
                     LQGDANLSAMGGDGAQVLIGNSGNNLLDGGNTGMDTLDGGAGNDTLRVSGGWEQPGAV
                     LTGGAGQDVFYFLGALSGDHSQGDTTVRITDFTQGEDVIGLNLLAGVAAPSSLITLTA
                     QTGDTLDSLLSQAASQANASQPSVLSQFEFAGDTYVVLDNNEMSNEVTLADLAFKLDG
                     AHALTLADFAITANTRQNIGGAGADVIRVAGYSHGQQTVTLTGNGGNDVFWIQSSYRG
                     DHATGSNTLTLSDFTSGADTLVLGLGNTALIKPDHITTLTVEAGDTLATLMARASHQT
                     TTYTQPALSQFVWGGDTYLVMDTTADPTWVSSNLAIKLSGAPTLSMADISFAG"
     gene            complement(1673..2608)
                     /gene="cysB"
                     /locus_tag="PKN55_04700"
     CDS             complement(1673..2608)
                     /gene="cysB"
                     /locus_tag="PKN55_04700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012696093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HNX70575.1"
                     /translation="MKLQQLRYLVEVAKQGLNVSEAAETLHTSQPGISKQIRLLEDEL
                     GIQVFIRNGKRVVAVSEPGKQVLRIAERILREAKNLKRVGEEFASEAEGSLIIATTHT
                     QARYALPETIRAFRQHYPNVRLQLKQGNPTQICDMVVAGEADFAIATEGILLYKELAA
                     LPCHSWNRSVVAPHGHPLTELDRPITLADIGAYPIITYDTAFTGRTRINHAFEQAGVT
                     PNLVLTAIDTDVIKTYVGIGLGVGIIASMGYEPDRDHDLVALDASHLFEPSITKIGIR
                     KDAYLRGFAYTFIELFAPHLTRAKVDEAILAGQQP"
     gene            2859..3566
                     /locus_tag="PKN55_04705"
     CDS             2859..3566
                     /locus_tag="PKN55_04705"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011137121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HNX70576.1"
                     /translation="MAYADKLPATEQLLASIARDFSPAVFANSLGAEDMVLTDLIAKL
                     GLGIEIFSLDTGRLPAETYTLLQQVGERYPSHPVTVYFPQTAATEQYVREHGINGFYQ
                     SVEQRKACCAVRKIEPLKRALAGRQAWITGLRRQQSLTRVDLADREFDADNGLWKFNP
                     LIEWTEAEVWEYLRAHDVPYNALHDQHYPSIGCAPCTRAISMGEDVRAGRWWWENPDS
                     KECGLHLKASPLRRPGS"
     gene            3680..5341
                     /locus_tag="PKN55_04710"
     CDS             3680..5341
                     /locus_tag="PKN55_04710"
                     /EC_number="1.8.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018151003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050311 - sulfite reductase (ferredoxin)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HNX70577.1"
                     /translation="MYRYDEVDFRLVNERVAQFRDQTRRYLAGELSEDEFRPLRLMNG
                     LYVQRHAPMLRVAVPYGMLSSRQVRKLAHIARTYDRGYGHFTTRQNLQFNWPALETVP
                     DILAELAEVEMHAIQTSGNCIRNTTTDQFAGVAADELVDPRPYCEIIRQWSTFHPEFT
                     YLPRKFKIAVSGAEQDRAATLVHDIGLHAVRNAEGEVGFRVIVGGGLGRTPMIGSVIR
                     EFLPAKHILTYLDAILRVYNRFGRRDNKYKARIKILVKAMTADGFAAKVEEEWSHLKD
                     GPATVPDGEFARMAEFFTAPAYETLNADDPAFTAKLAESPAFARWVARNTLKHKVAGY
                     RAVTLSLKATGVPPGDATAEQLDAVADWADAYSFGELRVSHEQNLILADVRETDLFAL
                     WEQARAAGFATPNIGLLSDIVCCPGGDFCSLANAKSIPIAAAIQQRFDNLDYQHDIGE
                     LELNMSGCMNACGHHHIGHIGILGVDKQGSEWYQVSLGGSQGEHASLGKVIGPSFAQD
                     AMPDVIEKIIDVYLAQRHADERFIDTFRRIGVEPFKEKVYAQAAH"
     gene            5322..5807
                     /locus_tag="PKN55_04715"
     CDS             5322..5807
                     /locus_tag="PKN55_04715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011137119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF934 domain-containing protein"
                     /protein_id="HNX70578.1"
                     /translation="MPKPLIKDRQIIDDAWTLVRDAEAGAPAGPAILPLALWKTLRDS
                     RAAADTGVWLAPADDPAELAGAVAELPVIAVDFPQFADGRGYSIGRLLRERYGFAGEL
                     RAIGDVLRDQLYPLEQVGFNAFSLREDRDPAKDIAGLHDFSLGYQQTFLRQQPLFRHA
                     R"
     gene            6032..6667
                     /locus_tag="PKN55_04720"
     CDS             6032..6667
                     /locus_tag="PKN55_04720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011137118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNX70579.1"
                     /translation="MNKLKLSGLAGALLVALFSAQAHAAKPGYVVDQSTDAVTRNSYG
                     ECWKTTYFDKSNGLVECGDAVAPAPAAKPVAAAPVVSKERIALSAKVLFGFDKANLRP
                     QAKAELDPVVAKIKQHGANLQGVVVEGHTDYLGSDKYNQKLSESRATTVKSYFVANGI
                     PADKVTAVGKGETETKMTETCKAKKLSYAKLKDCLEPDRRVEVEITGVQAK"
     gene            6983..7654
                     /gene="queC"
                     /locus_tag="PKN55_04725"
     CDS             6983..7654
                     /gene="queC"
                     /locus_tag="PKN55_04725"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011137054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HNX70580.1"
                     /translation="MLSGGQDSTTCLYWALRRFDHVEAVTFDYGQRHRVELAAAARIA
                     ADAGVRQHLLPINTFSALGGNALTDAAMAPETGLRADGDSELPNTFVPGRNLIFLTFA
                     AALAWQRQLDHLVVGVAQTDYSGYPDCRAPTLAALQQALTLGLDRAIAVHAPLMQLSK
                     ADTVRLAAEVGALPALAHSHTCYHGAVPPCGECAACQLRARGFAEAGVEDPLLARCRE
                     EAACR"
     gene            7960..8262
                     /locus_tag="PKN55_04730"
                     /pseudo
     CDS             7960..8262
                     /locus_tag="PKN55_04730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010434567.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
     gene            8239..8625
                     /locus_tag="PKN55_04735"
     CDS             8239..8625
                     /locus_tag="PKN55_04735"
                     /inference="COORDINATES: protein motif:HMM:NF013411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HNX70581.1"
                     /translation="MRETATAGSQFNGHTHRIWKTQRFEAATPFDAHGRYTGHSYSLR
                     LHLSGQLDEVMGWVQDFGDVKTRFKPFYQQLDHYPLDQVDGLSVANCAGIAQWAAAKL
                     ANTPELCRVDVYQRPGEGSLFSVEAA"
     gene            8622..9254
                     /gene="queE"
                     /locus_tag="PKN55_04740"
     CDS             8622..9254
                     /gene="queE"
                     /locus_tag="PKN55_04740"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012696605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase"
                     /protein_id="HNX70582.1"
                     /translation="MSYTVKEIFYSLQGEGHHSGRPAVFCRFSGCNLWSGREEDRARA
                     QCTFCDTDFVGVGPDGGKFADAQALARRIASHWPAQGGGTPYVVCTGGEPLLQLDALL
                     IDALHAQGFEVAVETNGTLPAPRGLDWICVSPKGKARLAQTWGHELKLVYPQADCTPE
                     QVAHLDFRHFYLQALDDPAHPEHQQAALDYCLAHPRWKLSLQTHKWLRIR"
     gene            9325..9762
                     /gene="queF"
                     /locus_tag="PKN55_04745"
     CDS             9325..9762
                     /gene="queF"
                     /locus_tag="PKN55_04745"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012696604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HNX70583.1"
                     /translation="MTLHEHLQHLGSGTTYRYDQPDAALLERFPNPFARGELNPAGVT
                     GTLNISCPEFTSLCPVTGQPDFATIVIDMEPDQWCVESKSLKLYLGSFRMHGEFHEAC
                     VCRIANDLVALLQPRWLRVEGRFTPRGGIPLWPKAEYRRPDAS"
     gene            9807..10655
                     /gene="serB"
                     /locus_tag="PKN55_04750"
     CDS             9807..10655
                     /gene="serB"
                     /locus_tag="PKN55_04750"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018150210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
                     /protein_id="HNX70584.1"
                     /translation="MSSLNLIIQGKPLAMDVLAELAVEAGSPAIEPLSKTAWRFLDAK
                     ESSKEWVSQACRELECDWAYVPAGLTLSHFGLWMTDMDSTLIQIECIDEIAGMLGLKD
                     KVAAITERSMAGELDFSQSLRERVALLAGLKETTLKVVYEKKVKLNPGAKELVAACQA
                     KGVKIGLISGGFTYFTNRLKQELGLDYAIANTLEVVNGRLTGMVKGDIIDAQAKADWL
                     LKLRGELGLRPDQVFATGDGANDLKMLAAAGVGIAYHAKPVVRHQAHVALSYSGLDGV
                     INLFAD"
     gene            10666..11145
                     /locus_tag="PKN55_04755"
     CDS             10666..11145
                     /locus_tag="PKN55_04755"
                     /inference="COORDINATES: protein motif:HMM:NF028133.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HNX70585.1"
                     /translation="MPLPTLNTLSFDAELVCRLSSQPISAAQQHDTRLALRLLGATEQ
                     TSEDPVLLRIEAKLDVCLAWLGHSTQQQHGDSRLCRLGLEQFAWRSQADEPSGAATLT
                     FYPDTHCPLPLTFPVHCQTQADGHTIASLAPALDEASADLWAHFVFRQHRRQRQQQA"
BASE COUNT         2115 a         3693 c         3491 g         1988 t
ORIGIN      
        1 aacacaccct gacagataag gcatacatca tgaccacctc tattagcgct tctaccaact
       61 acaccctggg cgcgggggaa gacaatctgc tgctcaccgg ctcggccgac ctgaagggca
      121 caggcaacca tgccaacaac caactggaag gcaacgatgg cgccaatgta ctgataggcg
      181 ggctgggcta cgaccagctc tatgccggcg caggcgatga tgtgctgatc ggcaatcagg
      241 ccggtgtcat gcaagacaat gacgccgacg cgctctttgg ccaccagggc aatgacaccc
      301 tgtatctgaa tggcctggat tatgccgacg gcgacgaagg cgccgacacc tactgggtga
      361 ctggcggttc caacagcatt caggataacg gcccggatgc cggtgtggat gtggtgaagg
      421 ccttcacctc ggtctccatg ccaagctact atgcagtgca agacccgcgc tatggcaatg
      481 tgtcggcgta tggcattgaa cgggtggagc tgcaaggcga tgccaacctg agcgccatgg
      541 gtggcgatgg cgcgcaagtg ctgatcggca acagcggcaa taatctgctg gatggcggca
      601 ataccggcat ggataccctg gatggcggcg ccggcaacga caccttgcgc gtatccggcg
      661 gctgggagca acctggcgcc gtgctgaccg gcggggcagg gcaagatgtg ttttattttc
      721 tgggtgcgct gagtggcgac cacagccagg gcgacaccac cgttcggatc accgatttca
      781 cccagggtga agacgtgatc ggccttaacc tgctggccgg cgtggcggcc cccagcagcc
      841 tgatcacgct gacggcgcaa accggtgaca ccttggacag cctgcttagc caggcggcca
      901 gtcaggccaa cgccagccag cccagcgtgc tgagtcagtt tgaatttgct ggcgacacct
      961 atgtggtgct ggacaacaac gagatgagca acgaagtgac gctggcggat ctggccttca
     1021 agctggatgg cgcgcacgcg ctgaccctgg ccgattttgc catcaccgcc aacacccgcc
     1081 agaatattgg cggcgcaggc gcggatgtga tccgtgtggc cggctactcg catggtcaac
     1141 aaaccgtcac cctgaccgga aacggtggca atgatgtgtt ctggattcaa agcagctacc
     1201 gtggcgacca cgccaccggc agcaatacgc tcaccctcag cgacttcacc tccggcgccg
     1261 acacgctggt gctgggcttg ggcaacacag ccctgatcaa accagaccat atcaccacgc
     1321 tgacagtcga agccggcgac accctggcca ccctgatggc gcgcgccagc caccagacca
     1381 ccacctacac gcaaccggcg ctgagccagt ttgtctgggg cggcgacacc tatctggtga
     1441 tggacaccac cgccgacccg acctgggtca gcagcaatct ggccatcaag ttgtctggcg
     1501 cacccacctt gagcatggcg gacatcagct ttgccgggta aacgccgctg atatgcacag
     1561 acaaaccgca tgcgctacgc ggtttgtctg cgtgagcgcc actgacaaaa caccacgccc
     1621 ctggcgctga acgcacaggg gcgtggtgtt ttaccgccca gccaagccga tctcagggct
     1681 gctggccggc cagaatggct tcgtccacct tggcgcgggt caggtgcggg gcaaacagtt
     1741 caataaaggt ataggcaaag ccgcgcaggt aggcgtcttt gcggatgccg atcttggtga
     1801 tggacggctc gaacagatgg ctggcgtcca gcgccaccag atcatgatcg cggtcgggct
     1861 cgtaacccat cgaagcgatg atgcccacgc ccaggccaat gccgacatag gttttgatca
     1921 cgtcggtgtc gatggcggtc agcaccagat tgggggtgac gccggcttgc tcaaaggcgt
     1981 gattgatgcg ggtgcggcca gtaaacgccg tgtcgtaggt gatgatggga taggcgccga
     2041 tgtcggccag ggtaatcggg cgatccagct cggtcagcgg gtggccgtgc ggggccacca
     2101 cgctgcggtt ccagctgtgg cagggcaggg cggccagctc tttgtacagc aggatgcctt
     2161 cggtggcgat ggcgaagtcc gcctcgccgg ccaccaccat atcgcagatc tgcgtggggt
     2221 tgccttgctt gagttgcagg cgcacattcg ggtagtgctg gcgaaacgcc cggatggttt
     2281 ctggcagcgc atagcgcgcc tgggtgtgcg tggtggcgat gatcaggctg ccttcggcct
     2341 cgctggcgaa ctcttcgccc acgcgcttga ggtttttggc ttcgcgcaga atgcgctcgg
     2401 cgatgcgcag cacctgcttg cccggctcgg acaccgccac cacgcgcttg ccgttgcgaa
     2461 taaacacctg aatgcccaat tcgtcttcca gcaggcggat ttgcttgctg atgcccggct
     2521 gggaggtgtg cagggtttcg gcggcttcag agacgttcaa gccttgcttg gcgacttcga
     2581 ccaaataacg gagctgttgc agtttcatgg ggcgtggttc aatcagacag gggcggcggg
     2641 caggctgggc catggccgcc agggttggca aataaccagc tgctcttaaa tgctaacaca
     2701 ttattctttt gcgtgcgctt gagctttggg taagatgccg cttcggtttc atcccccagt
     2761 gcgcgcccag cgcgcggcgc gggttggccg cagatgggca tggcgtcgcc ggcggcggct
     2821 cggcccgcat gacgattccc actctggagc tttgccgcat ggcctacgcc gacaaacttc
     2881 ccgccaccga gcagctgctt gccagcattg cccgtgattt ctccccggcg gttttcgcca
     2941 acagcctggg cgcggaagac atggtgctca ccgacctgat cgccaaactg ggcctgggca
     3001 ttgaaatctt cagcctggac accggccgcc tgccggcgga aacctacacc ctgctgcaac
     3061 aagtgggcga gcgctacccc agccacccgg tgacggtgta cttcccgcag accgccgcca
     3121 ccgagcaata cgtgcgcgaa cacggcatca acggctttta ccagtcggtg gagcagcgca
     3181 aggcgtgctg cgcggtgcgc aagatcgagc cgctcaagcg cgcgctggcc ggccgccagg
     3241 cctggatcac cggcctgcgt cgtcagcagt cgctgacccg cgtggatctg gccgaccgcg
     3301 agttcgacgc cgacaacggc ctgtggaagt tcaatccgct gattgaatgg accgaagccg
     3361 aagtgtggga atacctgcgc gcccacgacg tgccgtacaa cgccctgcac gaccagcatt
     3421 acccctccat cggctgcgcg ccctgtaccc gggcgatttc catgggcgaa gacgtccgcg
     3481 ccggccgctg gtggtgggaa aaccccgact ccaaggagtg tggcctgcat ctgaaagcct
     3541 cgccgctgcg ccgccccggc agctaagcgc ccgccaaccc gactcagtgc agcgtgcctg
     3601 acgagacgcg cgctgcactg agtgttgtgc gccgcgccaa gccgcgctcc gcccctgatc
     3661 cttatccccg agacgccaca tgtatcgtta cgacgaagtt gactttcgcc tggtcaatga
     3721 gcgggtcgcc cagttccgcg accaaacccg ccgctacctg gccggcgaac tgagcgaaga
     3781 tgaattccgc ccgctgcggc tgatgaacgg cctgtatgtg cagcgccatg cgccgatgct
     3841 gcgcgtggcg gtgccctacg gcatgctgtc ctcgcgccag gtgcgcaagc tggcgcacat
     3901 tgcccgcact tacgaccgtg gctacggcca cttcaccacc cgccagaatc tgcaattcaa
     3961 ctggccggcg ctggaaaccg tgcccgacat cctggccgag ctggccgagg tggaaatgca
     4021 cgccatccag acctcgggca attgcatccg caacaccacc acggaccagt ttgccggcgt
     4081 ggccgccgac gagctggtgg acccgcgccc gtattgcgaa atcatccgcc agtggagcac
     4141 cttccacccg gaattcacct acctgccgcg caagttcaag attgccgtgt ccggcgccga
     4201 gcaagaccgc gccgccaccc tggtgcacga catcggcctg cacgccgtgc gcaatgccga
     4261 gggcgaagtg ggctttcgcg tgatcgtcgg cggcggcctg ggccgcacgc cgatgattgg
     4321 cagcgtgatt cgtgaattcc tgccggccaa gcacatcctg acctatctgg acgccattct
     4381 gcgcgtgtac aaccgctttg gccgccgcga caacaagtac aaggcgcgca tcaagattct
     4441 ggtcaaggcg atgaccgccg acggctttgc cgccaaggtg gaagaagaat ggtcgcacct
     4501 gaaagacggc ccggccaccg tgccggatgg cgaattcgcc cgcatggccg agtttttcac
     4561 cgccccggcg tatgaaaccc tcaacgccga cgacccggcc tttaccgcca agctggccga
     4621 atcgccggcc tttgcccgct gggtggcgcg caacaccctg aagcacaagg tggccggcta
     4681 tcgcgccgtc accctgtcgc tgaaggccac tggcgtgccg ccgggcgacg ccaccgccga
     4741 gcagctggac gccgtggccg actgggccga cgcctacagc tttggcgagc tgcgcgtgtc
     4801 gcacgagcaa aacctgattc tggccgatgt gcgggaaacc gatttgttcg cgctgtggga
     4861 gcaagcccgc gccgccggct tcgccacgcc gaatatcggc ctgctgtctg acatcgtctg
     4921 ctgcccgggt ggggatttct gctcgctggc caacgccaag tccatcccca tcgccgccgc
     4981 catccagcag cgctttgaca atctggacta ccagcacgac atcggcgagc tggagctgaa
     5041 catgtccggc tgcatgaacg cctgcggcca ccaccacatc ggccatatcg gcattctggg
     5101 cgtggataag cagggcagcg agtggtatca ggtgtcgctg ggcggcagcc agggcgagca
     5161 cgccagcctg ggcaaggtga ttggcccgtc gttcgcccag gacgccatgc ccgacgtgat
     5221 cgaaaaaatc atcgacgtgt acctggccca acgtcacgct gacgagcgtt ttatcgacac
     5281 attccgccgc attggcgtgg aaccgttcaa ggagaaggtc tatgcccaag ccgctcatta
     5341 aagatcgcca gatcatcgac gacgcctgga ccctggtgcg tgacgccgag gccggcgcgc
     5401 cggcaggccc ggccattctg ccgctggcgc tgtggaaaac cctgcgcgac agccgcgccg
     5461 ccgccgacac cggcgtctgg ctggccccgg ctgacgaccc ggccgaattg gccggcgcgg
     5521 tggccgaact gccggtgatt gccgtggatt tcccgcaatt cgccgatgga cgcggctaca
     5581 gcattggccg actgttgcgt gaacgctacg gttttgccgg cgaattgcgt gcgattggcg
     5641 atgtgctgcg cgaccagctc tatccgctgg aacaagtcgg ttttaacgcc ttttcgctgc
     5701 gagaagaccg cgatccggcc aaagatatcg ccggtttgca cgacttcagc ctgggctacc
     5761 agcagacttt cctgcgccag cagcctttgt tccgccatgc gcgctgacag gatggctgca
     5821 acacaggcct gatagcgtta ttgtgtgtgg cgagtcagag acatgttgct tgctcgccac
     5881 agttgtggat gccggtcgaa ttgacaatat atagtcaaaa acctatattc ccggtaactg
     5941 cgagccaccg tttcctgttg cttgcggcac gcacaacaca tgctatctgc ttaatacact
     6001 ctctaacgaa agaggcacac ggatgaatac catgaacaaa ctgaaactca gcggtctggc
     6061 tggcgcgctg ctcgtcgctc tgttttccgc ccaggcccac gccgccaagc cgggctatgt
     6121 ggttgatcaa agcaccgacg ctgtgacccg caacagctac ggcgaatgct ggaagaccac
     6181 ctacttcgac aagtccaatg gcctggttga gtgcggcgac gctgttgccc cggctccggc
     6241 cgccaagccg gttgccgccg ctccggtggt gagcaaggag cgcatcgccc tgtccgccaa
     6301 ggtgctgttc ggcttcgaca aggccaacct gcgtccgcaa gccaaggctg agctggaccc
     6361 ggttgttgcc aagatcaagc aacacggcgc caacctgcaa ggcgttgtgg tcgaaggcca
     6421 caccgactac ctgggctccg acaagtacaa ccagaagctg tcggaaagcc gcgccaccac
     6481 cgtgaagtcc tacttcgtgg ctaacggcat cccggccgac aaggtgaccg ccgtcggcaa
     6541 gggcgaaacc gaaaccaaga tgaccgaaac ctgcaaggcc aagaagctgt cctacgccaa
     6601 gctgaaggac tgcctggagc cggatcgtcg cgtggaagtg gaaatcaccg gcgtgcaagc
     6661 caagtaattt cccctgcctc gtgcaaaaaa ccccgcgcct gtcgcggggt ttttttatgt
     6721 ccgctcagta caccacgggc ttgccgcctg caccccatcc gggtgaagcc ctctccatgc
     6781 cctcagcgcg ccatcgtgcc cctttattgc gtattttccc gtatactgcc cgcctttgac
     6841 gccaacaagg cgttgcgcgc cgggtgagac cgatcccgag cgcgggcaag cccttcgccg
     6901 tgaagggcgc acaccgactg tttatcggac cctgccaatg atgtcacctg ctaccgacca
     6961 ttccggtggg cgggcgcttg tcgtgctttc gggcggacaa gactcgacca cctgcctgta
     7021 ttgggcgctg cgccgctttg accatgtcga agccgtcact ttcgactacg gccaacgcca
     7081 ccgcgtggag ctggccgccg ccgcgcgcat tgccgccgac gctggcgtgc gccagcacct
     7141 gctgcccatc aacaccttta gcgccctggg cggcaatgcg ctgaccgacg ccgccatggc
     7201 ccctgaaacg ggcctgcgcg ccgatggcga cagcgagttg cccaacacct ttgtgcctgg
     7261 ccgcaatctg attttcctga cctttgccgc cgcgctggcc tggcaacgcc agctcgatca
     7321 cctggtggtg ggcgtggcgc aaaccgatta ttccggctac ccggattgcc gcgcgcccac
     7381 actggcggcg ctgcaacagg cgctgaccct gggcctggat cgcgccattg ccgtgcacgc
     7441 gccgctgatg cagctgagca aggccgacac cgtgcgcctg gccgccgagg tgggcgcctt
     7501 gccggcgctg gcgcacagcc acacctgtta tcacggcgcc gtgccgccat gcggcgaatg
     7561 cgccgcctgc cagctgcgcg cgcgcggctt tgccgaagcc ggagtggaag acccgctgct
     7621 ggcgcgctgc cgcgaggagg ccgcatgccg ctgatgcacg ccgccagttg cggttttgag
     7681 gcagcgcgca gcgtgcgcaa cgtcgccgac ctgcgccgct gcctgcatgg ccacagcttt
     7741 ttgtgcagcg cgcgctgggc cgacggcgcg cccgatgtgg gcgcggcgct gtccactgcg
     7801 ctggcgccgc tggattatgc cgacctcaac caggcggttg ccgtgccgga cgacgccagc
     7861 ctgctgggct ggattgccgg gcatctgccg cacgctgacg gcctgtggct gcgcagcgcg
     7921 ccagatcgcg gcgtgttgcg cgccagcgcc caggcgccgc tgctgcactg gctgcacgcc
     7981 gacttcgaag ccgcccacca gctgcccaat gtgccgcccg gccaccagtg cggccgcctg
     8041 catggtcacg gctttggtgt gacgctgtgc gccgccgcca gccacgccga gctggagcaa
     8101 gcctgggcgc ggctgcgccc gctgctgcat caacgcatgc tcaacgatat tcccggcctg
     8161 gaaaacccca ccagcgaggt gatcagcgcc tggctgtggc ggcaattggc cgatgtgctg
     8221 gcgctggacc atgtgatcgt gcgggaaacc gccaccgccg gcagccagtt caatggccac
     8281 actcaccgca tctggaaaac ccagcgcttt gaagccgcca cgccgttcga tgcccatggc
     8341 cgctacaccg gccacagcta cagcctgcgc ctgcacctga gcggccagct ggacgaggtg
     8401 atgggctggg tgcaggattt tggcgacgtc aaaacccgct tcaagccgtt ttaccaacag
     8461 ttggaccatt atccgctgga ccaagtggac gggctgagcg tggccaattg cgccggcatt
     8521 gcccaatggg cggcggccaa gctggccaat accccggaac tgtgccgggt ggatgtctat
     8581 cagcgccccg gcgaaggcag cctgttcagc gtggaggcgg catgagctac accgtcaaag
     8641 aaatttttta ctccttgcag ggcgaaggcc accacagtgg ccgaccggcg gtgttttgcc
     8701 gtttcagcgg ctgcaatctg tggagcgggc gcgaagaaga ccgcgcacgc gcgcaatgca
     8761 ccttctgcga caccgatttt gtcggcgtcg gcccggatgg cggcaaattt gccgacgcgc
     8821 aggcgctggc gcgccgcatt gccagccatt ggccggcaca gggcggcggc acgccctatg
     8881 tggtgtgcac cggcggcgag ccgctgttgc agctggatgc cttgctgatc gacgccttgc
     8941 acgcgcaagg ctttgaagtg gcggttgaga ccaatggcac gctgccggcg ccgcgcgggc
     9001 tggactggat ttgcgtcagt cccaaaggca aggcgcggct ggcgcaaacc tgggggcacg
     9061 agctcaagct ggtttacccg caggcggatt gcacgccgga acaggtggcg catctggatt
     9121 ttcgccattt ttacctgcaa gcgctggacg atcccgccca cccggaacac caacaggcgg
     9181 cgctggacta ttgtctggcg cacccacgct ggaagctgtc gctgcaaacg cacaaatggc
     9241 tgcgcattcg ctgacggcaa gttttgctgt tatttccgcc gaggccacgt cgcttcggcc
     9301 gcattcacct gtaaagacct ggccatgact ctgcacgaac acctgcaaca cctgggcagc
     9361 ggcacgactt accgctacga ccaacccgac gccgcgctgc tggagcgctt tcccaacccg
     9421 tttgcccgtg gcgagctcaa cccggccggg gtgaccggca cgctgaacat cagttgcccg
     9481 gaattcacca gcctgtgccc ggtgaccggc cagcccgact tcgccaccat cgtcattgat
     9541 atggagcccg accaatggtg cgtggagtcc aaatcactca agctgtatct gggctcgttc
     9601 cgcatgcatg gcgagtttca cgaagcctgc gtgtgccgca tcgccaatga tctggtggcg
     9661 ctgttgcagc cgcgctggct gcgcgtggaa ggtcgcttca ccccgcgcgg cggcattccg
     9721 ctgtggccca aggcggaata ccgccgcccg gacgccagct gacggtataa ttccggcttt
     9781 gtttgcattg caggagccgc aaggccatgt cgtcgttgaa tctgatcatc cagggcaaac
     9841 cgctggccat ggatgtgctg gccgaactgg ccgtggaagc cggctcgccg gccattgaac
     9901 ccttgagcaa aacggcctgg cgttttctcg acgccaagga gagcagcaag gaatgggtgt
     9961 cgcaagcctg ccgcgagctg gaatgcgact gggcctatgt gccggccggc ctcaccctca
    10021 gccactttgg cctgtggatg accgatatgg actccacgct gatccagatc gaatgcatcg
    10081 acgaaatcgc cggcatgctc ggcctgaaag ataaagtcgc cgccatcacc gagcgctcga
    10141 tggcgggcga gctggatttt tcgcaatcac tgcgtgagcg ggtggccttg ctggccgggc
    10201 tgaaagaaac cacgctgaaa gtggtgtacg agaaaaaggt caaactcaac cctggcgcca
    10261 aagaactggt tgccgcctgc caggccaagg gcgtcaagat cggcctgatt tccggcggct
    10321 tcacctattt caccaaccgg ctcaaacaag agctgggcct ggattacgcc atcgccaaca
    10381 ccctggaagt ggtcaacggc cggctgaccg gcatggtcaa gggcgacatc atcgacgcgc
    10441 aagccaaggc cgactggctg ctcaaactgc gcggcgagct gggcctgcgc cccgaccagg
    10501 tgtttgccac gggcgacggc gccaatgacc tgaaaatgct ggccgccgcc ggcgtgggca
    10561 ttgcctacca cgccaagccg gtggtgcgtc atcaggcgca tgtggcgctc agctacagcg
    10621 gcctggacgg cgtgatcaac ctctttgccg actgacacac tcaccatgcc gctgccgacg
    10681 ctgaacaccc tcagttttga cgccgaactg gtctgccgtc tgagcagcca gccgatcagc
    10741 gccgcgcaac agcacgacac ccgcctggcg ctgcgcctgc tgggcgccac cgagcaaacc
    10801 agcgaagacc cggtgctgtt gcgcatcgaa gccaagctgg acgtctgcct ggcctggctg
    10861 ggccacagca cacaacaaca gcacggcgac agccggctat gccgactggg gctggagcaa
    10921 tttgcttggc gcagccaagc cgacgagccc agcggcgcag caacgctgac gttttacccg
    10981 gatacacact gtcccctgcc gttgaccttt ccggtgcatt gccaaaccca ggcagatggc
    11041 cacaccatcg ccagcctggc gccggcgcta gacgaggcca gcgcagactt gtgggcgcat
    11101 tttgtgtttc gtcagcaccg ccggcaacgt cagcagcagg cctgaaatac tttgccaaac
    11161 ccataaaaaa acgctgcggc agaatatgcc ccagcgtttt tttatggctg agtttcaaat
    11221 taacacccga gttaaaaata caaaaccccc tgtagcgtga gctacagggg gctgtaatag
    11281 gagcctg
//
LOCUS       DAOPYX010000061        11173 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_6678_length_11173_cov_9.246537, whole genome shotgun sequence.
ACCESSION   DAOPYX010000061 DAOPYX010000000
VERSION     DAOPYX010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 11173)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11173)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11173
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6678_length_11173_cov_9.246537"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(381..2252)
                     /locus_tag="PKN55_04760"
     CDS             complement(381..2252)
                     /locus_tag="PKN55_04760"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNX70586.1"
                     /translation="MSSPDPSIEIARQALKRLTARRQLPTPENYERAYAEVAGQLLPA
                     SSRPVHPLVAALGQFLDELQPGSDAAGQIRINRLKQAIFEQDWSGIPAMLQQHLTLQV
                     EQQTVGQRWGELVLNLIKQWDLRQPGLSSAMKKEALERVVANYGYQPTLFYEKLSNLL
                     TNWALPLAASQASPPSALSLAGFQFDEALPPPAASGTAASASAAPQMTQINQIHGQPV
                     SKMDEPLSRAWKVVLDEALRYGVVPWLTPYPELRESIEEPLLALPGIDNEQALEVFSR
                     QLKKVWIKTDLQLQHDGRIVNGLASLLSLLLHNLRELSGQDQSLIGQISAIETVLAQQ
                     PLNVRDIYAVEASLKDVIYKQGVIKHSLDAATQAMRDMVNTFVARLSVMTDDTSQFQQ
                     KIGDYAQKIQAADNVIALSEVIDTLMADTKSVQTSLANSRGELEAARVEVDAATQRIR
                     ELEAALDEASMQVKEDQLTGAYNRRGLDEMFAREKDRSAQSGQPLSMVLLDVDNFKQL
                     NDHYGHLTGDQVLKHLVDTISANLRPNDIVARLGGEEFVLLLPATAAEAAAQLTERLQ
                     RQLTRAYFMADQDRLVITFSAGVTVWKLGEDEVDTIERADQAMYRAKLAGKNRVHIA"
     gene            complement(2348..2662)
                     /gene="ftsB"
                     /locus_tag="PKN55_04765"
     CDS             complement(2348..2662)
                     /gene="ftsB"
                     /locus_tag="PKN55_04765"
                     /inference="COORDINATES: protein motif:HMM:NF002058.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HNX70587.1"
                     /translation="MRALTLFLLVLLLLLQWSLWLGKGSWLRVWQLDSQLTTLRTSNE
                     KLVDRNLALAAEVSDLKQGSSAIEERARSELGMIKRGEVFFQLLEPRPAAAPAAAKPS
                     AP"
     gene            complement(2673..3956)
                     /gene="eno"
                     /locus_tag="PKN55_04770"
     CDS             complement(2673..3956)
                     /gene="eno"
                     /locus_tag="PKN55_04770"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012696032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HNX70588.1"
                     /translation="MSSIIDVVAREILDSRGNPTVEADVLLESGVMGRAAVPSGASTG
                     EKEALELRDGDKGRYLGKGVLKAVENINTEICEAIIGLDATDQTFIDKTLLQLDGTDS
                     KSRLGANALLAVSMAVARAAAEDAGLPLYRYLGGAGQMSMPVPMMNVINGGAHANNSL
                     DIQEFMIIPVGAKTFREALRCGAEIFHTLKKICAEQGHSTAVGDEGGFAPNLAKNEDA
                     IKLILQAIDQAGYVAGDDVLIALDCASSEFYKDGKYHLKGEGLALSSEEFANYLATLC
                     DNYPIISIEDGMSEHDWAGWKILTDKLGDRVQLVGDDVFVTNPKILSEGIKAGICNSL
                     LVKINQIGSLTETLQAVELAKRSGYTSVMSHRSGETEDSTIADLAVATNCMQIKTGSL
                     SRSDRMAKYNQLLRIEEELGEAAVYPGRAAFYHLK"
     gene            complement(4071..4922)
                     /gene="kdsA"
                     /locus_tag="PKN55_04775"
     CDS             complement(4071..4922)
                     /gene="kdsA"
                     /locus_tag="PKN55_04775"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014086214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HNX70589.1"
                     /translation="MHLCGFEVGLHRPLFLIAGPCVIESEQMALDTAGQLKEIAQAVG
                     MPFIYKSSYDKANRSSGKSFRGLGMDEGLRILAEVKRQIGVPVLTDVHTTEEIPTVAS
                     VVDVLQTPAFLCRQTDFIRAVATAGIPVNIKKGQFLAPGDMKNVVDKAREAAIEAGSD
                     GQHIMVCERGVSFGYNNLVSDMRSLAIMRDTGCPVVFDATHSVQLPGGQGTASGGQRE
                     FVPVLARAAVAAGVAGLFMETHPDPAQALSDGPNAWPLPRMRELLATLKSLDDLVKSS
                     PLAEQSL"
     gene            complement(4982..6616)
                     /locus_tag="PKN55_04780"
     CDS             complement(4982..6616)
                     /locus_tag="PKN55_04780"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008953152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HNX70590.1"
                     /translation="MTQYIFVTGGVVSSLGKGIAAASIAAILESRGLTVTMLKLDPYI
                     NVDPGTMSPFQHGEVFVTEDGAETDLDLGHYERFIKAKMKKSNNFTTGQVYESVITKE
                     RRGDYLGGTVQVIPHITDEIKAKVYAGAGDADVAIVEIGGTVGDIESLPFLEAIRQIR
                     SNLGRKNTLYVHLSYVPFIHTAGEIKTKPTQHSVKELREIGIQPDILLCRMDRPLPDD
                     ERRKIALFCNVEENAVIGCYDASSIYKIPAQLHEQGIDDIIAEHLNLTLPPADLSVWD
                     GIVDAIEHPQREVNIAMVGKYVDLTESYKSLSEALNHAGIHTRSKVNIHYIDSEEIER
                     DGAASLAGMDAILVPGGFGKRGVEGKILAVKFARERNIPYMGICLGMQIALIEYARDV
                     AGMAGANSTEFDLETDFPVVALIDEWVNHDGKIEKRDENSNLGGTMRLGGQQCDLEPG
                     SLAARVYGATQIVERHRHRYEVNNYYVPRLEAAGLKISGRSSGHEQLVETIELPNHPW
                     FFACQFHPEFTSTPRDGHPLFKAYIEAAIAHQAARG"
     gene            6804..7037
                     /gene="rpmB"
                     /locus_tag="PKN55_04785"
     CDS             6804..7037
                     /gene="rpmB"
                     /locus_tag="PKN55_04785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HNX70591.1"
                     /translation="MARVCKVTGKRPMTGNNVSHANNKTKRRFLPNLQYRRFWVESEN
                     RWVRLRVSNAALRTIDKVGIDVVLADLRARGEL"
     gene            7079..7234
                     /gene="rpmG"
                     /locus_tag="PKN55_04790"
     CDS             7079..7234
                     /gene="rpmG"
                     /locus_tag="PKN55_04790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012696028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HNX70592.1"
                     /translation="MRDKIKLESTAGTGHFYTTTKNKRTMPGKMEIKKFDPVARKHVI
                     YKETKLK"
     gene            7525..8103
                     /locus_tag="PKN55_04795"
     CDS             7525..8103
                     /locus_tag="PKN55_04795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016393899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM165/GDT1 family protein"
                     /protein_id="HNX70593.1"
                     /translation="MEAFLFSTGFVALAEIGDKTQLLALLLAARFKRPTPIIAGIFVA
                     TVLNHALAGSLGSWLTALVPADIMRWVLGGSFLAMAVWLLIPDSIDDEDTRLQDKLGV
                     FGATVIAFFLAEMGDKTQIATVALAARFDSLMGVVMGTTAGMMLANVPAVLLGEAAAK
                     RIPVKWVHGCAALLFAVLGVVVLSGVQLGFMG"
     gene            complement(8277..9464)
                     /locus_tag="PKN55_04800"
     CDS             complement(8277..9464)
                     /locus_tag="PKN55_04800"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014085800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HNX70594.1"
                     /translation="MKPLAEIVWQRLTGLMPGELKKAELPWLATPFQHLSLLANRRAT
                     MIVNRVRLFAFLFAVLTPMWIVVDFLVFASPLWWQLALMRVLASAAFASLVLFYQPRG
                     NLLDAYRAMALLFLIPTLFYVASYNLMGAHELAGLSAAVSSGYAFLPFVLLAGLAIFP
                     LTLIENLILASPILIAQIVVAGLRWPHLDLPAFIGTFWLLVLITGVSALAGMSQLAFM
                     IALVRQATRDPLTGVFSRSSGEEMLELQFILASRSGAPLAVAFVDLDHFKSINDHYGH
                     EAGDKVLIAMTRNIQACLRRGDMLTRWGGEEFLLIMPNTDLEQAVQALARLREHGLGA
                     RPEGAPVTASIGVAERLGDALDDWRMLVELADHRMYYAKQHGRNQVCAALPDGQDPLV
                     KAA"
     gene            10198..>11173
                     /locus_tag="PKN55_04805"
     CDS             10198..>11173
                     /locus_tag="PKN55_04805"
                     /inference="COORDINATES: protein motif:HMM:NF014696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HNX70595.1"
                     /translation="MLMIDAQKRLTHPVIDDLCRLITQHRWEPRFEEAFALVAGYQIA
                     GFPRIATLTDYFTWLVELLEWVPCENSQGREIYNRICEFYFVLDQAPLRELQNPQLPA
                     APGAPLTPLSAWMVDFARAWGQFLDTPDSLTAESLQSFYAAPAYNMGEYITAPGGWKS
                     FNQFFARHVKPGYRPVAAPQDDHILVSPADSTFVGAWAIGADSHITVKSLRWSLDELL
                     ADSPYRERFRSGLFMHSFLNTTDYHRLHTPVSGRVLESRVIHGQVYLEVNAQPKETEG
                     DAHELVRERVLHAEDGTGYQFSQARGLLVLDSPVGLVAVLPVGMCQVSS"
BASE COUNT         2184 a         3358 c         3370 g         2261 t
ORIGIN      
        1 ggggtgttcg aaaaaacttg accaggacag tctgcggtcg tgcgcctagc caggaacgct
       61 gcgctgagtt ttgttagcca ctctaagcgc cgttcacaaa accccctggc gttgtggtgt
      121 gaccttgccg tacttgttgc actgtctgcg gtcacacgcc tagccaggat cgcttcgctg
      181 aattttatga gcgactctaa gtagatttac ggattcacag caaaaattat agccgagcca
      241 atgcgcgctg gatgagtagg ctattgattt gcgacaaatc tgcagcaagc caggtatgac
      301 gggcaaaaaa tggcgaaaag ctcaggaaaa tagcgaatca ggcaagcgtc atccggccat
      361 gcgcctgagc gcatatccgc tcaggcaatg tgcacgcggt ttttaccggc cagtttggcg
      421 cgatacatcg cctggtcggc gcgttcgatg gtgtccactt cgtcttcgcc cagcttccac
      481 acggtgacgc cagcgctgaa ggtgatgact aatcggtcct ggtcggccat gaaataggcg
      541 cgggtgagct ggcgttgcag gcgctcggtc agttgggcgg cggcttcggc ggcggtggcc
      601 ggcagcaaga gcacaaactc ttcaccgccc agacgggcga cgatgtcgtt ggggcgcaag
      661 ttggcgctga tggtgtccac cagatgcttg agcacctgat caccagtcag gtggccatag
      721 tggtcgttga gctgcttgaa gttgtccacg tccagcagca ccatggacaa aggctggccg
      781 ctttgcgccg agcggtcttt ctcgcgggca aacatttcgt ccagaccacg gcggttgtag
      841 gcgccagtga gctggtcttc cttgacctgc atgctggctt catccagcgc ggcttccagc
      901 tcgcggatgc gctgggtggc ggcatccact tcgacgcggg cggcttccag ctcgccccga
      961 ctgttggcca ggctggtttg caccgacttg gtgtcggcca tcagcgtgtc gatcacttcc
     1021 gacagggcaa tgacattgtc ggctgcctgg attttctgcg cgtaatcgcc gattttctgc
     1081 tggaactggc tggtgtcatc ggtcatcacc gacaggcgcg cgacaaaggt gttgaccatg
     1141 tcgcgcatgg cctgggtggc ggcgtccagg ctgtgcttga tcacgccttg cttgtagatc
     1201 acgtccttga ggctggcttc cacggcgtag atgtcgcgca cattcaaggg ttgctgggcc
     1261 agcacggttt cgatggcgct gatctggccg atcaatgatt gatcctgacc agaaagctcg
     1321 cgcagattgt gcagcagcag gctgagcagc gaagccaggc cattgacgat gcggccgtcg
     1381 tgctgcaatt gcagatcggt tttgatccag acttttttca gctggcgcga gaacacctcc
     1441 agcgcctgct cgttgtcgat gcccggcaag gctagcaagg gttcttcaat gctttcacgc
     1501 agttccggat aaggcgtcag ccagggcacc acaccgtagc gcagcgcttc gtccagcacc
     1561 actttccagg cgcggctgag cggctcgtcc atcttgctga ccggctggcc gtggatctga
     1621 ttgatctggg tcatctgcgg tgcagcagag gcagacgccg cagtgcccga cgctgccggc
     1681 ggcggcagcg cttcgtcaaa ctgaaaaccg gccaggctca gcgccgacgg cggtgatgcc
     1741 tggctggcgg ccagcggcaa ggcccagttg gtcagcagat tgctgagctt ttcgtaaaac
     1801 agggtgggct ggtagccata attggccacc acgcgctcca gcgcttcttt tttcatggcg
     1861 ctggacaagc ccggttggcg caaatcccat tgcttgatca ggttgagcac cagttcgccc
     1921 caacgttggc caacggtttg ttgctccacc tgcagggtga ggtgttgttg cagcatggcc
     1981 ggaatgcccg accaatcctg ttcgaagata gcctgtttga ggcggttgat gcgaatctgc
     2041 ccggccgcat cgctgccagg ctgcaattca tccagaaact gccccaatgc cgccaccagc
     2101 gggtgcactg gccggctgga tgcgggcaac agctgtccgg ctacttcagc gtaagcacgc
     2161 tcgtagttct ccggagtcgg cagctgcctg cgcgcggtta accgcttgag cgcctggcga
     2221 gcaatttcaa tggaaggatc aggcgacgac atcgcgcgct cgagagtcac acatgcgggc
     2281 agtgtaatcc tgtccgccag tcttgttaag gcaccataca ccgacgcgct ggcacatcat
     2341 ggcgttttca cggtgcggaa ggcttggccg cagccggggc ggcggcgggg cgaggctcca
     2401 gcaactggaa aaacacctcg ccgcgcttga tcatgcccag ttcgctgcgc gcgcgctcct
     2461 cgatggccga ggagccctgc ttgaggtcgc tgacttccgc cgccagcgcc agattgcggt
     2521 caaccagctt ttcattgctg gtgcgcaggg tggtgagctg gctgtccagc tgccacacgc
     2581 gcaaccagct gcccttgccc agccacaatg accattgcag cagcaagagc agcaccagca
     2641 gaaaaagcgt caacgcgcgc atgcgacccg gattacttca ggtggtaaaa cgcagcgcgg
     2701 ccggggtaca cggcggcttc gccgagttct tcctcgatgc gcagcagctg gttgtacttg
     2761 gccatgcggt cagaacgcga cagcgagccg gtcttgatct gcatgcagtt ggtggccacg
     2821 gccaggtcgg cgatggtgct gtcttcggtt tcgcccgagc ggtggctcat cacgctggtg
     2881 tagcccgagc gcttggccag ttccacggct tgcagggttt cggtcagcga gccgatctgg
     2941 ttgattttca ccaagaggct gttgcagatg ccggccttga tgccttcgga caggattttc
     3001 gggttggtga cgaacacatc gtcgcccacc agctgcacgc gatcgcccag cttgtcggtc
     3061 aggatcttcc agccggccca gtcgtgctcg ctcatgccgt cttcgatcga gatgatcggg
     3121 tagttgtcgc acagggtggc caggtagttg gcgaattctt cgctggacag cgccaggcct
     3181 tcgcccttca ggtggtactt gccgtctttg tagaattcgc tggaagcgca gtccagggca
     3241 atcagcacgt cgtcgccagc cacatagccg gcctggtcga tggcttgcag aatcagcttg
     3301 atggcgtctt cgttcttggc caggttcggg gcaaagccgc cttcgtcgcc cacggcggtg
     3361 gagtggccct gctcggcgca gatttttttc agggtgtgga agatttccgc cccgcagcgc
     3421 agcgcttcgc ggaaggtttt ggcgcccacc gggatgatca tgaattcctg gatgtccagg
     3481 ctgttgttgg cgtgcgcgcc gccgttgatc acgttcatca tcggcaccgg catcgacatc
     3541 tggccagcgc cgcccaggta gcggtacagc ggcaggccgg cgtcttcggc ggcggcgcgg
     3601 gccacggcca tggacacggc caggagggcg ttggcgccca gacggctctt gctgtcggtg
     3661 ccgtccagtt gcagcagggt cttgtcgatg aaggtctgat cggtggcgtc caggccgatg
     3721 atcgcttcgc agatttcggt gttgatgttt tcaacggcct tgagcacgcc cttgcccagg
     3781 tagcggccct tgtcgccatc gcgcagttcc agggcttctt tttcgccggt ggaggcgccg
     3841 gacggcacgg cggcgcggcc catcacgccg gattccagca gcacgtcggc ttccaccgtc
     3901 gggttgccac gcgaatccag aatctcgcgc gcgacgacat caataatcga actcatggtt
     3961 ttccccaatg cagcaatgca agtacgtgat caaaaacaat gcccgggcgc agcgtcaggc
     4021 cggcccgcag gcaaacggat agcgccgcgc ccgcaagtgg acgcggcgga ttataacgat
     4081 tgttccgcca aaggcgagga ttttaccaag tcgtccagcg acttgagcgt ggccaagagc
     4141 tcgcgcatgc gcggcagcgg ccaggcgttg ggcccgtcag acagcgcctg ggccgggtct
     4201 ggatgggttt ccataaacag cccggccacg cctgcggcca cggccgcgcg cgccagcacc
     4261 ggcacaaact cgcgctgacc gcccgaagcc gtgccctggc cgccaggcag ttgcaccgag
     4321 tgggtggcgt caaacaccac cgggcaaccg gtgtcgcgca tgatggccag gctgcgcatg
     4381 tccgacacca gattgttgta gccaaacgaa acgccgcgct cgcacaccat gatgtgctgg
     4441 ccatcgctac cggcctcaat cgctgcttcg cgcgccttgt ccaccacgtt tttcatgtca
     4501 ccgggggcca gaaactggcc ctttttgatg ttcaccggga tgccagcagt ggccacggcg
     4561 cggatgaaat cagtttgccg acacaagaac gctggcgttt gcagcacgtc caccacgctg
     4621 gcgacggtgg gaatttcttc cgtggtgtgc acgtcggtga gcaccggcac gccaatctgg
     4681 cgcttgacct cggccagaat gcgcaggcct tcgtccatgc ccaagccacg gaaggacttg
     4741 ccggaactgc ggttggcctt gtcgtagctg gatttgtaga taaacggcat gcccacggcc
     4801 tgggcaattt ctttgagctg gccggcggtg tccagcgcca tttgctcgct ttcgatgaca
     4861 caggggccgg caatcagaaa cagcgggcga tgcagcccga cttcaaaccc gcacagatgc
     4921 atgaacggct tctccagaaa acagccgacg gggcatatgc cccgtcgtca ggtcgaacag
     4981 cttagccgcg ggcggcttga tgggcgatgg cggcctcgat ataggccttg aacagcggat
     5041 ggccatcacg cggggtggag gtgaattccg ggtggaactg gcaggcgaag aaccacgggt
     5101 ggttcggcag ctcgatggtt tccaccagct gctcatggcc gctggagcgg ccgctgatct
     5161 tcagcccggc ggcttccaga cgcggcacgt agtagttgtt gacttcgtaa cggtgacgat
     5221 gacgctcgac gatctgagtg gcgccgtaca cgcgcgccgc cagcgaaccc ggctccaggt
     5281 cgcactgctg gccgcccagg cgcatggtgc cgcccaggtt ggagttttcg tcgcgctttt
     5341 cgatcttgcc gtcgtggttc acccattcgt cgatcagcgc caccaccggg aagtcggttt
     5401 ccaggtcgaa ttcggtggag ttggcgcctg ccatgccggc cacgtcgcgg gcgtattcga
     5461 tcagcgcgat ctgcatgccc aggcaaatgc ccatgtaggg gatgttgcgc tcgcgggcaa
     5521 atttcaccgc cagaatcttg ccttccacgc cgcgtttgcc aaagccgccc ggcaccagga
     5581 tggcgtccat gccagccagc gaggcggcgc cgtcgcgctc gatctcttcc gagtcgatgt
     5641 agtggatatt gaccttgctg cgggtgtgga tgccggcgtg gttcagcgct tcggacaagg
     5701 atttgtacga ttcggtgagg tccacatact tgccgaccat ggcgatgttc acttcgcgct
     5761 gcggatgctc gatggcgtcg acaatgccat cccacaccga cagatcagcc ggcggcaggg
     5821 taaggttcag gtgttcggcg atgatgtcgt cgatgccttg ctcgtgcagc tgggccggaa
     5881 tcttgtagat gctgctggcg tcgtaacagc cgatcacggc gttttcttcg acattgcaga
     5941 acagggcaat cttgcggcgc tcgtcgtccg gcagcgggcg atccatgcgg cacagcagga
     6001 tgtccggctg gatgccgatt tcgcgcagtt ccttcaccga gtgctgggtc ggcttggtct
     6061 tgatttcgcc agcggtgtgg atgaacggca cataggacag atgcacgtac agggtgttct
     6121 tgcggcccag gttggagcgg atctgacgga tggcttccag gaagggcagc gattcgatgt
     6181 cgcccaccgt gccgccgatt tcgacaatcg ccacgtcagc atcgccagcg ccagcgtaca
     6241 ccttggcctt gatttcgtcg gtgatgtgcg gaatcacctg cacggtgccg cccaggtagt
     6301 cgccacggcg ctctttggtg atcaccgatt cgtacacctg gccggtggtg aagttgttgc
     6361 ttttcttcat cttggccttg atgaagcgct catagtggcc aaggtcgagg tcggtttcgg
     6421 cgccgtcttc ggtgacgaac acctcgccat gctggaacgg gctcatggtg cccggatcga
     6481 cgttgatgta cggatcgagc ttgagcatgg tcacggtaag accgcgagat tccagaattg
     6541 ctgcgatgga tgcagcagca atgcctttac ccagcgagga cacaaccccg ccggtcacga
     6601 aaatgtattg agtcatggga atgctggcct gaacgaatcc ggaattttat cgtgaaatcc
     6661 ctcgcgcgcc aaatgcttgg cggtatcggg ccctcggcag ccccgtcaga cggcctccgg
     6721 cagccacacg cacgcaacac ttgagcagcc gcctgatttt atgatataaa gcgtgttttt
     6781 actggtttct gggagtaact caaatggcgc gagtttgcaa agtcaccggc aagcgtccga
     6841 tgaccgggaa caatgtttcc cacgccaaca acaagaccaa acgtcgtttc ttgccgaatc
     6901 tgcaatatcg ccgtttctgg gtcgaaagcg aaaaccgctg ggtgcgcctg cgcgtgtcca
     6961 acgctgccct gcgcaccatt gacaaggttg gcatcgacgt tgtgctcgcc gatctgcgtg
     7021 cacgcggcga actgtaatct gtccaccgcg accgtcactg aattaaaggc taatcaccat
     7081 gcgcgacaag atcaaactgg aatcgaccgc cggcaccggt cacttctaca ccaccaccaa
     7141 gaacaagcgg accatgcccg gcaagatgga aatcaaaaag ttcgacccgg ttgcccgcaa
     7201 gcacgtgatc tacaaggaaa ccaagctgaa gtaattcagc cgcggttgcc cgtgctgcta
     7261 aaaagccctg atgcgttgcg tcagggcttt tgtttttttg ccgcgagaat ttgctgctgc
     7321 ggcgcaatgc cgctacaatg ccgccactca ctgggggagt agccggcctg tcgccagaca
     7381 ggcgcccgcg tcaacacact tgccgaaccc ggcatggcgc gcgacatcca cggatgcggc
     7441 aagacccatg acctgcgctc gctgccttaa gggggccgcg gcgacacagg tcatggcgcg
     7501 tctggccccc tggatatttt catcatggaa gctttcctgt tctccaccgg ctttgtcgcc
     7561 ctggccgaaa tcggcgacaa gacccaactg cttgccctct tgctcgccgc ccgcttcaaa
     7621 cgcccgaccc ccatcattgc cggcattttt gtcgccaccg tgctcaacca cgccctcgcg
     7681 ggcagcctgg gcagctggct gaccgcgctc gtgccggcag acatcatgcg ctgggtgctg
     7741 ggcggctcgt ttcttgccat ggcggtgtgg ttgctgattc cggattccat cgacgacgaa
     7801 gacactcgac tgcaagacaa gctgggcgta tttggcgcca ccgtgattgc ttttttcctg
     7861 gccgagatgg gcgacaagac gcaaatcgcc accgtggcgc tggccgcgcg ttttgacagc
     7921 ctgatgggcg tggtgatggg caccacagca ggcatgatgc tggccaatgt gccggccgtg
     7981 ctgctgggcg aagccgctgc caaacgcatt ccggtgaaat gggtgcacgg ctgtgcggcc
     8041 ctgctgtttg ccgtgttggg cgtggtcgtg ctgagcgggg tgcaactggg ttttatgggc
     8101 taagaagcac ccacacaatg tgcctggtgt tgtggcgtga ccttgccgca cgatttgtac
     8161 cgtctgcggt cacacgccag gccaggatga cttcgctgca ttttgtgggc gacgcggggt
     8221 caggcgcccg cgcgcatcaa gccagccggg cgccagactg cagcgcccgg atcaattcag
     8281 gcagccttga ccaagggatc ctgcccatct ggcagggccg cgcacacctg gttacgacca
     8341 tgctgcttgg cgtaatacat acggtgatcg gccagctcaa ccagcatgcg ccagtcgtcc
     8401 agcgcatcac ccaaacgctc ggcaacacca atgctggcag tcaccggagc gccttccggc
     8461 cgcgcaccca gcccatgttc gcgcagccgc gccagtgctt gcacggcttg ctccagatcg
     8521 gtattgggca tgatcagcaa aaattcttcg cccccccaac gggtaagcat atcgccacgg
     8581 cgcaaacacg cctgaatatt gcgggtcatg gcaatcagca ccttgtcccc agcctcatgg
     8641 ccatagtggt cattgatgct cttgaagtgg tccagatcga caaacgccac cgccagcggc
     8701 gcaccactgc ggctggccag aatgaattgc agctccagca tttcttcacc gctgctacgt
     8761 gaaaacaccc cggtcagcgg gtcgcgggtg gcctgacgca ccagggcaat cataaaggcc
     8821 agctggctca tgccggccag cgccgacacc ccggtaatca gtaccagcag ccagaaagtg
     8881 ccaataaaag ccggcaagtc cagatgcggc cagcgcaagc cggccaccac aatctgggca
     8941 atcaggatcg ggctggccag aatcaggttt tcaatcagcg tcagcggaaa aatcgccagg
     9001 ccagccagca atacaaatgg cagaaaggca taaccagaac tgaccgcagc cgacagcccg
     9061 gccagctcat gcgcccccat caggttgtat gacgccacat aaaacaaagt aggaatcaga
     9121 aacagcaaag ccatggcccg ataggcatcc agcagattgc cacgcggctg gtaaaacagc
     9181 accagactgg caaaggcagc actggccagc acacgcatca gcgccaattg ccaccacaag
     9241 ggcgacgcaa agaccagaaa atccaccacg atccacatcg gggtgagcac ggcaaacaaa
     9301 aaggcaaaca ggcgcacccg attgacaatc atggttgcgc gccggttggc cagcagggaa
     9361 aggtgctgga agggcgttgc cagccatggc aattccgcct ttttcagctc accgggcatc
     9421 agcccggtca ggcgctgcca tacgatttca gccaacggtt tcatcaggat tcccgctcca
     9481 ggtcaaaaca gtttgttgat ccacattgca tatagcatac ccgatatgcc atacgtacac
     9541 agattacccg cacaagcgcg gcttacaaaa ctcagcactg cattcctggt caggcgcgca
     9601 gccgcagaca gcataggttg tgcgcctaaa gccgcaccac aacgccagga gggttttgtt
     9661 agcggctcgt aagtgggcta ggcgtgggct gctcgcgcgc cctgcacgct aagtgaatct
     9721 gggcaaaacg gcaagtgcca gctggcacag gtttgatcta atcccacatc agccggaact
     9781 cccccaacga caagcccagg tcacacaagc gctgacgtag ccgttttctt cgaggctggc
     9841 gcggatgccg caatacatac tgtgcaggag atcaccctat cagcttcgtg gctgtgcaat
     9901 atgctggact tggcgaatca aaatagcttg tttgtgcaga ttttgcgtaa aaatgcctat
     9961 agaaaacata cgatgcgtaa ttttacttac gtaaaaccac ggattttata accattgtga
    10021 ataatgtttt ttcgtgacat catggttttt attgcgccaa ggctcaaata aaagccgcaa
    10081 aattatttta atagccaaat aggcaaaaag catacagatt gccggtcggc cttgtcgcag
    10141 cccccttggc gccgtgcgta gcacggcaaa gtgcactccg acaaaagagg ttcgtccatg
    10201 ttgatgatag acgcgcaaaa acgcctgacc cacccggtga tcgacgatct gtgccgactg
    10261 atcacccagc atcgctggga gccacggttt gaagaagcct ttgccctggt ggcgggctac
    10321 cagattgccg gctttccgcg cattgccacc ctgaccgact acttcacttg gttggtggag
    10381 ctgctggaat gggtgccttg cgaaaacagc caggggcgcg aaatctacaa ccgcatctgt
    10441 gagttttatt ttgtgctgga ccaagccccg ttgcgcgaat tgcaaaaccc gcagttgccg
    10501 gcagcgcccg gcgccccgct gaccccgctg tcggcctgga tggtggactt tgcccgcgcc
    10561 tgggggcagt ttctggatac gccagactcg ctcactgcag aatcgctgca aagtttttac
    10621 gcagcgccgg cctacaacat gggtgaatac atcaccgcgc cgggcggctg gaaaagcttt
    10681 aaccagtttt ttgcccgcca cgtcaaaccc ggctaccggc cggtggcggc gccgcaggat
    10741 gaccatattc tggtgtcgcc agccgactcc acctttgtcg gcgcctgggc gattggcgcg
    10801 gactcccata tcaccgtcaa gagcctgcgc tggtcgctgg atgaattgct ggccgacagc
    10861 ccctaccgcg agcgctttcg cagtggcctg ttcatgcaca gctttttgaa caccactgat
    10921 taccatcgcc tgcacacccc agtgtccggc cgcgtgctgg agtcacgggt gattcatggt
    10981 caggtctact tggaagtcaa cgctcagccc aaagaaactg aaggcgacgc acatgaactg
    11041 gtgcgcgagc gcgtactgca cgccgaggac ggcaccggct atcaattctc gcaggcgcgc
    11101 ggcttgctgg tgctggatag cccggtgggc ttggtcgcag tactgccagt gggcatgtgt
    11161 caggtgtcgt cgg
//
LOCUS       DAOPYX010000062        11087 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_6756_length_11087_cov_13.489394, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000062 DAOPYX010000000
VERSION     DAOPYX010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 11087)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11087)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11087
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6756_length_11087_cov_13.489394"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1025)
                     /locus_tag="PKN55_04810"
     CDS             complement(<1..1025)
                     /locus_tag="PKN55_04810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014088667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNX70596.1"
                     /translation="MSTLIELKVPDIGGHSNVDVIEVFIKAGDSVAKDDALVTLETDK
                     ATMDVPADAAGVVKEVKIAVGSKVSEGDVIALIEASAAAAAPAAAPAAEAKPAAAPAA
                     APVAASHSGAADIECDVLVLGAGPGGYSAAFRAADLGLKVVLVERYSTLGGVCLNVGC
                     IPSKALLHNAAVIDEVKHLAANGIKFAEPEIDIDMLRGYKEKVIGKLTGGLAGMAKAR
                     KVQVVRGVGQFLDPHHLQVETTDGQGQDKTGAKQVVKFKNAIIAAGSRVVNLPFIPQD
                     PRIVDSTGALELKSVPKKMLIIGGGIIGLEMGTVYSTLGARLDVVEMLDGLMQGADRD
                     LVKVWQK"
     gene            complement(1086..2720)
                     /gene="aceF"
                     /locus_tag="PKN55_04815"
     CDS             complement(1086..2720)
                     /gene="aceF"
                     /locus_tag="PKN55_04815"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004285328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004742 - dihydrolipoyllysine-residue
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HNX70597.1"
                     /translation="MSNLIEVKIPDIGGHSGVDVIEVFVKPGDSIKVDDALVTLETDK
                     ATMDVPSSAAGVVKEVKIAVGSKVSEGDVVLLLEAGAADAPAAPAAAPAPAAAPAAPA
                     AAAAPAASANVDIVVPDIGGHSNVDVIEVAVKVGDEIKVDDTLITLETDKATMDVPAT
                     AAGVVTAVAIKVGDKVSEGALIVTVAAAGAAPAPAAAPAPAPAAAPVVAAPAPVAAAA
                     APAPVAAPAALPAINEASFATAHAGPSVRRLARELGVDLGRVSGSGRKGRIVEQDVKS
                     FVKGVMQNPALLAPTAAPAAGSGVGLDLLPWPKVDFAKFGPIETKPLSRIQKISGANL
                     ARNWVMIPHVTFNDDCDITELEDFRKTIGKEWEKSGLKISPLAFIIKAAAEALKAFPT
                     FNSSLDGDNLVLKQYYHIGFAADTPNGLVVPVIKDADKKGLKQIAKELTDLSALARDG
                     KLKPTDMQGATFTISSLGGIGGTGFTPIVNAPEVAILGVCKSQIKPVWNGKEFTPRLM
                     CPLSLSFDHRVIDGAAAARFTVYLGKLLSDVRRLIL"
     gene            complement(2750..5425)
                     /gene="aceE"
                     /locus_tag="PKN55_04820"
     CDS             complement(2750..5425)
                     /gene="aceE"
                     /locus_tag="PKN55_04820"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011134081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HNX70598.1"
                     /translation="MVNPTLPNDIDPQETKEWIEALESVLAADGPERAHYLLEKMIDK
                     ARRRGAYLPFTATTAYLNTIPVGKEQRSHGNHELEHRIRSIIRWNAAALVLRAGKKDL
                     ELGGHIASFQSAATLYDVGFNHFWRAPNDEQEGDLIYIQGHSAPGMYARAFLEGRLSE
                     QDLDSFRQEVDGKGLSSYPHPWLMPDFWQFPTVSMGLGPLCAIYSARFMKYLASRGLI
                     DAAKAEQRKVWAFLGDGETDEVESLGAIGMAGREKLDNLIFVINCNLQRLDGPVRGNG
                     KIIQELEGEFRGSGWNVLKVIWGGRWDPLLAMDTQGLLKQRMEECVDGDYQTFKSKDG
                     AYVREHFFGKYPELREMVANMSDDDIWALNRGGHDPHKVYAAYYEATHNANGRPTVIL
                     AKTIKGYGMGRAGEAMNIAHQAKKMDLDSIRLFRDRLGIPIPDDQLEKAPYYKPAEDS
                     PEMQYMRARRSALGGYLPSRKPVKAPMTVPGLHAFDAMLKDSEGREFSTTMAFVRMLG
                     TLLKDKNISKQIVPIVPDESRTFGMEGMFRQYGIWSTQGQNYVPQDADQLMFYKESKD
                     GQMLQEGINEPGAMSSWIAAATSYANHGIPMIPFYIYYSMFGFQRIGDLAWAAGDLRA
                     RGFLLGGTAGRTTLNGEGLQHEDGHSHIQAGLIPNCISYDPTYAHELAVILQDGMRRM
                     YVDQEDVFYYITLMNENYPHPALPAGSEEGILKGAYLLKDGGDAEVKVQLMGSGTILR
                     EVEAAADLLRNDFGIAADVWSVTSFNLLNRDGRETARWNLLHPTETPREAYLTRALAG
                     RSGPFIAATDYIRAYADQVREFVPGRYTVLGTDGFGRSDSRQNLRKFFEVDRHYVVVA
                     ALKALADEGKLPAARVAEAMTRYGIDADKPASWLV"
     gene            complement(5850..7373)
                     /locus_tag="PKN55_04825"
     CDS             complement(5850..7373)
                     /locus_tag="PKN55_04825"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014088670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HNX70599.1"
                     /translation="MKPRALLSLLLSASLLCSPLAAQADALALPDLGDASQTTLSPQA
                     EQKLGRQIMAMIRESGEMLDDPEVELYLNQLGRRLSSASPELGQNFQFFPMLDPQINA
                     FALPGGYIGVHTGLIYTAQSESELASVIGHEMAHVVQHHIARSMVQQGNNQLLAIAGL
                     LVAILAASVSKNSQVSAAAVNAGTGMAIQNQLTFSREFEREADNIGMQILSRAGFDAR
                     AMPMFFERMQRVNRVNDNQALAFLRTHPVTGERISAGQDRAAQLPVKLVADSPAFLLV
                     REKCRVLQLGPMEAVKFYQHALAQRQFNNEAAIWYGLAVAKLKLGDANGAQSALNEAS
                     QRMGPQPMLLSFAGAIRLHQRDYPGARALFRDAVRRYPDARYLAYGEVDAAQAQGDAA
                     GVAALLKPLSVRYASDPELWQRVARQYADHDRLRYHQALGNAYYLQNKPSAALEQYQL
                     ASQAPGDDFYVRSSIEARIRELQTQLDEARKENGGKPLPRERFQDQHIHTPLAAHRH"
     gene            complement(7581..7994)
                     /locus_tag="PKN55_04830"
     CDS             complement(7581..7994)
                     /locus_tag="PKN55_04830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018151521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX70600.1"
                     /translation="MMIVAAGLVGLLFGLGLIVAGMANPAKVLAFLDVAGAWDPSLAL
                     VMGGAIGVALPAFALSRRRERGWLGARFDWPRATQIDRRLLLGGALFGVGWGMAGVCP
                     GPALVLLGAGVPQALGFVAAMLAGMWLVGKLWPAK"
     gene            complement(8010..8441)
                     /locus_tag="PKN55_04835"
     CDS             complement(8010..8441)
                     /locus_tag="PKN55_04835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018151520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE family protein"
                     /protein_id="HNX70601.1"
                     /translation="MTIDWIHFTPWAALAGGALIGLAAALLWWLTGRIAGISGLVGGL
                     LTAPSDWAFRLAFTLGLIAAPLLWRLFAPLPEIQLSASFAWMLAAGLVVGVGVKLGSG
                     CTSGHGVCGLARLSPRSLAATLSFMAAGFITVWLLRHGLGG"
     gene            complement(8501..8788)
                     /locus_tag="PKN55_04840"
     CDS             complement(8501..8788)
                     /locus_tag="PKN55_04840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018747558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HNX70602.1"
                     /translation="MEVLRDNSERAASLLKSLANPDRLLLLCQLVEGERSVTELEALT
                     GIRQPTLSQQLGVLRNEELVATRRDGKWVYYRIASEDALAILGTLHQIYCA"
     gene            complement(8934..9443)
                     /locus_tag="PKN55_04845"
     CDS             complement(8934..9443)
                     /locus_tag="PKN55_04845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014088675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix DNA-binding protein"
                     /protein_id="HNX70603.1"
                     /translation="MSPSLNIVSSSHLVSERSAELSEFEFGLMVVSNAFNRWMVRCMA
                     AAGEKDMTAMDVALLHHVNHLGRKKKLADICFVLNIEDTHVVSYALKKLVRAGHVASE
                     KIGKEVLFSTTPAGTALCERYREVRERCLIGALVESGASNAEIGNTAQLLRVLSGLYD
                     QAARAGASL"
     gene            9574..10242
                     /gene="pxpB"
                     /locus_tag="PKN55_04850"
     CDS             9574..10242
                     /gene="pxpB"
                     /locus_tag="PKN55_04850"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008952061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HNX70604.1"
                     /translation="MSQAAPHYYSLGESAVALALPAPATLAQQRRIWRLAASLAGAPG
                     VLDIVPGMNNLTVSYHPLDFDPDHAFDCLRQHWAALGAADDDAPARQVDIPVCYGGEA
                     GPDLAEVARHTGLTPAEVVKQHCAAEYQVFFMGFQPGFAYLGGLPEALATPRRAQPRT
                     RVPAGSVGIGGSQTGVYPASSPGGWQLIGHTRLKLFDPTATQPTLLQPGDRVRFVCEE
                     LRHA"
     gene            10235..>11087
                     /locus_tag="PKN55_04855"
     CDS             10235..>11087
                     /locus_tag="PKN55_04855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014088677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase family
                     protein"
                     /protein_id="HNX70605.1"
                     /translation="MLEILRPGVQTSVQDRGRRGLRHLGIAQAGALDMPALYRANSLL
                     GNDPDAAGLEITLGPVSVRFGRDAWLALSGAHFHPELNGRPIHTGQRVFAQAGHTLTL
                     SGCQYGMRTYLAVDGGIDAPLTLGARATDLQGGFGGVHGRALEAGDRLPLGAPRTLRD
                     GPGCRSPDWTPYVRALPGPDYASFSPSAQAAFWQRQWTVTPSSNRMGMRLRGTPLVRR
                     HNMDLLSHAVFPGVVQAPPDGQPIILLADAQTTGGYPCLATVIQADLWKLAQARPGAH
                     LHFVPCSA"
BASE COUNT         1817 a         3579 c         3631 g         2060 t
ORIGIN      
        1 atcttctgcc acaccttgac caggtcgcgg tcggcgccct gcatcaggcc gtccagcatt
       61 tccaccacgt ccaggcgcgc gcccagggtg gagtacaccg tgcccatttc caggccgatg
      121 atgccgccgc cgatgatcag cattttcttc ggcacggatt tcagttccag cgcgccggtg
      181 gaatcgacaa tgcgcgggtc ttgcgggatg aacggcaggt tcaccacgcg gctgccggcg
      241 gcgatgatgg cattcttgaa cttgaccact tgcttggcgc cggtcttgtc ctggccttgg
      301 ccgtcggtgg tttccacttg cagatgatgc gggtcgagga actggcccac gccgcgcacc
      361 acttgcacct tgcgcgcctt ggccatgccg gccaggccgc cagtgagctt gccgatgact
      421 ttttctttgt agccgcgcag catgtcgatg tcgatttccg gctcggcaaa cttgatgccg
      481 ttggcggcca ggtgcttgac ttcgtcgatc accgcggcgt tgtgcagcag cgccttggac
      541 gggatgcagc ccacgttcag gcacacgccg cccagcgtgc tgtagcgctc gaccagcacc
      601 actttcaggc ccagatcagc cgcgcggaaa gcggcggaat agccgccagg gccggcgccc
      661 agcaccagca catcgcattc aatatccgcc gcgccgctgt ggctggcggc caccggggca
      721 gccgccgggg cggcggcggg tttggcttcg gcggccgggg cggcagccgg cgctgctgct
      781 gctgcgctgg cttcgatcag ggcgatgaca tcgccttcgc tgaccttgct gcccacggcg
      841 attttgactt ccttgaccac gccggcggcg tcggccggca cgtccatggt ggctttgtcg
      901 gtttccaggg tgaccagcgc atcgtccttg gctacgctgt cgcccgcttt gatgaatacc
      961 tcgatcacgt ccacattgct gtggccgccg atatcgggga ctttcagttc aatcagggtg
     1021 ctcatgaatt ttcccaatcg cgtggccggc cggaacgcgt ccggccggca aaaggccagg
     1081 tgacgttaca ggatcaggcg acgcacatcg ctcagcagct tgcccagata cacggtgaag
     1141 cgggcggcgg cggcgccgtc gatcacgcgg tggtcaaacg acagcgacag cgggcacatc
     1201 aggcgcgggg tgaattcctt gccattccac accggcttga tctggctctt gcacacgccc
     1261 aggatggcca cttccggcgc attgacgatc ggggtgaagc cagtgccgcc aatgccgccc
     1321 aggctggaga tggtgaaggt ggcgccctgc atgtcggtgg gcttgagctt gccgtcgcgc
     1381 gccagcgcgg acaggtcggt cagttccttg gcgatttgct tgaggccctt cttgtcggcg
     1441 tccttgatca ccggcaccac cagcccattg ggcgtgtcgg cggcaaagcc gatgtggtag
     1501 tactgtttca gcaccaggtt gtcgccgtcc agcgagctgt tgaaggtcgg gaaggccttc
     1561 agtgcttcgg cggcggcctt gatgatgaag gccagcgggc tgatcttcag gccggatttt
     1621 tcccattcct tgccgatggt cttgcggaag tcttccagct cggtgatgtc gcagtcgtcg
     1681 ttgaacgtga cgtgcggaat catcacccag ttgcgcgcca gattggcccc ggagattttc
     1741 tggatgcgcg acagcggctt ggtttcaatc gggccaaact tggcgaagtc caccttcggc
     1801 cacggcagca ggtccaggcc cacgcccgag ccggcggccg gcgcggcggt cggcgccagc
     1861 agcgccgggt tttgcatcac gcccttgacg aaggacttca cgtcctgctc gacaatgcgg
     1921 cctttgcggc ccgagccgct cacccggccc aggtccacgc ccagctcgcg cgccagacgg
     1981 cgcaccgacg ggccggcgtg ggcggtggcg aagctggctt cgttgatggc cggcagggct
     2041 gccggggcag ccaccggggc cggggcagcg gcggccgcca ccggcgcggg cgcggcaaca
     2101 acgggcgcag ccgccggggc cggagccggc gcagcggcgg gcgcaggcgc tgcgcctgcg
     2161 gcggcgacgg tgacaatcag cgcgccttcg ctgactttgt cgcccacctt gatggccacg
     2221 gcggtgacca cgccagcggc ggtggccggc acgtccatgg tggccttgtc ggtttccagg
     2281 gtgatcaggg tgtcgtccac cttgatctcg tcacccactt tcaccgcgac ttcaatcacg
     2341 tccacattgc tgtggccgcc gatgtccggc accacgatgt ccacattggc gctggcggcc
     2401 ggagcagctg ccgctgccgg cgcagccggg gcggccgcag gggccggcgc cgcagcgggc
     2461 gcggccgggg cgtcggcggc gccggcttcc agcagcagca ccacatcgcc ttcgctgact
     2521 ttgctgccga cggcgatttt gacttccttg accacgccgg ccgcgctgga cggcacgtcc
     2581 atggtggctt tgtcggtttc cagcgtcacc agggcgtcgt ccaccttgat gctgtcgccc
     2641 ggcttgacga acacttcgat gacgtccacg ccgctgtggc cgccaatgtc ggggattttg
     2701 acttcaatca gattgctcat gttcttgtcc ttgtcgcgcg tcgtccgggt tacaccagcc
     2761 aggatgccgg cttgtcggcg tcgatgccgt agcgggtcat ggcttcggcc acgcgcgcgg
     2821 cgggcagttt gccttcgtca gccagcgcct tcaaggcggc caccaccaca tagtggcgat
     2881 cgacttcaaa gaatttgcgc aggttctgac ggctgtccga gcggccaaag ccgtcggtgc
     2941 ccagcacggt gtagcggccc ggcacgaatt cgcgcacttg gtcagcgtag gcgcggatgt
     3001 agtcggtggc ggcgatgaac gggccgctgc ggccggccag cgcgcgggtc aggtaggctt
     3061 cgcgcggggt ttcggtcggg tgcagcaggt tccagcgcgc ggtttcgcgg ccgtcgcggt
     3121 tcagcaggtt aaagctggtc acgctccaga catcggcggc aatgccaaag tcgttgcgca
     3181 gcaggtcggc ggcggcttcg acttcgcgca ggatggtgcc cgagcccatc agttgcacct
     3241 tcacctcggc gtcgccgccg tccttgagca ggtaggcgcc cttgaggatg ccttcttcgc
     3301 tgccggccgg cagcgccggg tgcgggtagt tttcgttcat cagggtgatg tagtagaaca
     3361 catcttcctg atcgacatac atgcggcgca tgccgtcttg cagaatcacc gccagctcgt
     3421 gggcgtaggt cgggtcgtag ctgatgcagt tggggatcag gccggcctga atgtggctgt
     3481 ggccgtcttc gtgttgcagg ccttcgccgt tcagcgtggt gcggccggca gtgccgccca
     3541 gcaggaagcc gcgcgcgcgc aggtcgcccg ctgcccaggc caggtcgcca atgcgctgga
     3601 agccaaacat cgagtaatag atgtagaacg gaatcatcgg gatgccgtgg ttggcgtagc
     3661 tggtggcggc agcaatccag ctcgacatgg cgcccggctc gttgatgcct tcctgcagca
     3721 tctggccgtc tttggattcc ttgtagaaca tcagctggtc ggcatcctgc ggcacgtagt
     3781 tctggccttg ggtagaccag atgccgtact ggcggaacat gccttccatg ccaaaggtgc
     3841 gcgattcatc cggcacgatc ggcacgattt gcttgctgat gtttttgtct ttcagcaggg
     3901 tgcccagcat gcgcacaaag gccatggtgg tggagaattc gcggccttcg ctgtctttga
     3961 gcatggcgtc gaaggcgtgc aggcccggca ccgtcatcgg cgctttcacc ggcttgcgcg
     4021 acggcaggta gccgcccagc gcgctgcggc gggcgcgcat gtattgcatt tccgggctgt
     4081 cttcggccgg cttgtagtac ggggcttttt ccagctggtc gtccgggatg gggatgccca
     4141 ggcggtcgcg gaacaggcgg atggagtcca ggtccatttt cttggcctgg tgggcaatat
     4201 tcatcgcctc gccagcgcgg cccatgccgt agcccttgat ggtcttggcc aggatcacgg
     4261 tcggacggcc gttggcgttg tgggtggctt cgtagtaagc cgcgtacacc ttgtgcgggt
     4321 cgtggccgcc acggttgagc gcccagatgt cgtcgtcgga catattggcg accatttcgc
     4381 gcagctcggg gtatttgcca aagaagtgct cacgcacgta agcgccgtcc ttggacttga
     4441 aggtctggta gtcgccgtcc acgcattctt ccatgcgttg cttgagcagg ccttgggtgt
     4501 ccatggccag cagcggatcc cagcggccgc cccagatgac tttcagcaca ttccagcccg
     4561 agccgcggaa ttcgccttcc agctcctgga tgatcttgcc gttgccgcgc accgggccgt
     4621 ccaggcgctg caggttgcag ttgatgacga agatcaggtt gtccagcttc tcgcggccgg
     4681 ccatgccgat ggcgccgagc gattcaacct cgtcggtctc gccgtcgccg aggaaggccc
     4741 acaccttgcg ctgctcggcc ttggcggcgt cgatcaggcc gcggctggcg aggtatttca
     4801 tgaagcgcgc ggagtagatc gcgcacaggg ggccgaggcc catcgacacg gtggggaact
     4861 gccagaaatc cggcatcagc cacgggtgcg ggtaggagga cagccccttg ccgtccactt
     4921 cctggcggaa gctgtccaga tcttgttcgc tcaggcggcc ttccaggaag gcgcgggcgt
     4981 acatgcccgg cgcagagtgg ccctggatat agatcagatc gccttcttgt tcgtcattgg
     5041 gcgcgcgcca gaagtggttg aagcccacgt catacagggt ggcggcggac tggaagctgg
     5101 cgatgtggcc gcccagctcc aggtcttttt tgccggcgcg cagcaccaaa gcggcggcgt
     5161 tccagcgaat gatggagcgg atgcggtgtt ccagctcatg gttgccgtgc gagcgctgtt
     5221 ccttgcccac cgggatggtg ttcaggtagg cggtggtggc ggtgaacggc agataggcgc
     5281 cgcgacggcg ggccttgtcg atcatttttt ccagcaggta gtgcgcgcgt tccgggccgt
     5341 cagcggccag gaccgattcc agcgcttcga tccattcttt ggtttcttgc gggtcgatgt
     5401 cgttgggcag tgttgggtta accatgtctc tccacctctt gagcgatagg gttgggcagc
     5461 ggcggcgatg cgcgccggaa atctgccgat tgcgggctgg ttgcccgtcg aatgtttgca
     5521 aacgaacaga aacgatcaga aaactctgtg ctctgcctcc tgtccagccc ggtctggccc
     5581 gcgctctgcc aggggtcacc cgattttttg gcgcgttcat gttgctggat gtcggatttt
     5641 gaacacaatg cattcgtttg agcaaacaaa taatcaaaac tgcttgcgca aaggcaaatt
     5701 gctgcggcgc gtcatgttgt tttttacaca cttgtgcgcg ccagtttcgt tatagcgtgg
     5761 tttttggcca gattcagcca atgggcatgt gagaaggtcg cgcagatgtc ggtggcgtgc
     5821 ggcgctgctt gctaggcgcc gcgccgccat caatggcgat gtgcagccag tggggtatga
     5881 atatgctggt cttgaaaacg ttcgcgcggc agcggcttgc cgccgttttc cttgcgcgct
     5941 tcgtccagct gggtttgcag ctcgcggatg cgcgcctcga tggacgagcg cacataaaaa
     6001 tcgtcgcccg gcgcctggct ggccagctgg tattgctcca gcgcggcaga aggcttgttt
     6061 tgcaagtaat aggcattgcc cagcgcctgg tgatagcgca ggcggtcatg atccgcatac
     6121 tggcgcgcca cccgctgcca gagctccggg tcgctggcgt agcgcaccga caagggtttg
     6181 agcagcgcag ccaccccggc ggcgtcgccc tgtgcctggg cggcgtccac ctcgccatag
     6241 gccagatagc gcgcatccgg gtaacggcgg acggcatcgc ggaacagcgc ccgtgcacca
     6301 gggtaatcgc gctgatgcag acggatggcg ccggcaaagc tcagcaacat cggctgcggg
     6361 cccatgcgct ggctggcttc gttcaaggcg ctctgtgcgc cgttggcgtc tcccagcttg
     6421 agcttggcca ccgccaggcc ataccagatc gccgcttcgt tattgaactg acgctgcgcc
     6481 agggcatgct gataaaactt caccgcctcc atcggcccca actgcaatac ccggcatttt
     6541 tcccgcacca gcaaaaacgc cgggctgtcg gccaccagct tgactggcag ctgggcggcg
     6601 cggtcctggc cggcgctgat gcgctcgcca gtcaccgggt gggtgcgcaa aaacgccagc
     6661 gcctggttgt cgttcacccg gttcacccgc tgcatgcgct cgaaaaacat cggcatggcg
     6721 cgggcgtcaa aaccggcgcg cgacaaaatc tgcatgccga tattgtcggc ttcgcgttca
     6781 aactcacggc tgaaagtcag ttggttctgg atggccatgc cggtgccggc attcacggcg
     6841 gcggcgctga cctggctgtt tttgctgaca ctggcggcca gaatcgccac cagcaggccg
     6901 gcaatcgcca gcagctggtt attgccctgc tgcaccatcg agcgagcaat atggtgctga
     6961 acgacatggg ccatttcgtg gccgatcacc gaggccagtt cggattcgct ttgcgcggtg
     7021 tagatcagcc cggtgtgcac gccgatatag ccgcccggca gggcaaaggc gttgatctgc
     7081 ggatccagca tgggaaagaa ctgaaaattc tgccccaact cgggcgaggc gctggaaagc
     7141 cgccggccca gttgattgag atacagctcg acctcaggat cgtccagcat ctcgccggat
     7201 tcgcggatca tcgccatgat ctggcggccg agtttttgct cggcctgcgg cgacagggtg
     7261 gtttgcgagg cgtcgccaag atcgggcagc gccagcgcgt cggcctgagc ggccagcggc
     7321 gagcacagca gagaggcgga gagcagcaga ctgagcaggg cgcgtggttt catggcgatg
     7381 atcataccgc ggcaaggtga ttgctggtca accctgatcg catgagactt tttgtgcagc
     7441 cggcgcagat gactcgctaa gcatcctttg catacgctgc ctttgcgccg cttacaaaac
     7501 cccgtgccgc gtgtctggcc agcgtgtcac gcagatagca aggtcgcgtc accaggcctg
     7561 gcgggttgtg taagccgtgc tcacttggca ggccacagct tgcccaccag ccacatcccg
     7621 gccagcatcg ccgcgacaaa gcccagcgcc tgcggcacgc ccgcgcccag cagcaccagc
     7681 gccgggcctg ggcacacccc tgccatgccc cagcccacgc cgaacagcgc gccgcccagc
     7741 agcaggcggc ggtcgatctg tgtggcgcgc ggccagtcaa agcgcgcgcc cagccaaccg
     7801 cgctcgcgcc ggcgcgacag cgcaaacgcc ggcagcgcca cgccaatcgc gccgcccatc
     7861 accagcgcca ggcttgggtc ccaggcgccg gccacgtcca gaaaggccag caccttggcc
     7921 gggttggcca tgccggccac gatcaggccc aggccaaaca gcaggcccac caagcccgcg
     7981 gcgacaatca tcatcggtcg attcatggtt tagcctccca gtccatggcg cagcagccat
     8041 acggtgatga agccggcagc cataaagctc agcgtggcgg ccagcgagcg cggcgacagc
     8101 cgcgccaagc cgcacacccc gtggccgctg gtgcagccgg agccgagttt gacgcccacg
     8161 cccaccacca aaccggcggc cagcatccag gcaaacgaag cagaaagttg aatctcaggc
     8221 aggggcgcaa acagccgcca cagcaggggg gcggcaatca ggcccagggt gaaagccagg
     8281 cgaaacgccc agtcagaagg ggcggtgagc aagccgccca ccaagccact gatgccggca
     8341 atgcgcccgg taagccacca cagcagggcg gcggccaggc caatgagcgc gccgccggcc
     8401 agcgccgccc agggggtaaa gtgaatccag tctattgtca tgacaagcgt cctgttcagt
     8461 acggttcccc ccgcctgaac aggccgctcg tcggttaagg tcaggcgcag taaatctggt
     8521 gcagcgtgcc cagaatcgcc agcgcgtctt cgctggcaat ccggtagtac acccatttgc
     8581 cgtcacgccg ggtggccacc agctcctcgt tgcgcagcac gcccagctgc tgcgacaaag
     8641 tgggctggcg gatgccggtg agcgcttcca gctcggtgac gctgcgttcg ccttcgacca
     8701 gttgacataa caacaacagc cgatcggggt tggccaggct tttcagcagg ctggccgccc
     8761 gttcagagtt gtcacgtaaa acctccatgg cggagccggg aagttcagcg gtaagttcat
     8821 gcggagtatt cacactatat acttttatat aatgattggc aaaaggcaag cggtttgctt
     8881 acccccaggt gaaaaccctt gctccaagta agagtaaagg cccggcgtcc acctcacaac
     8941 gaggcgcctg cccgcgcagc ctggtcatac aggccggaga gcacgcgcag caattgcgcg
     9001 gtgttgccga tttcggcatt ggaggcgccg ctttccacca gcgcgccgat caggcagcgt
     9061 tcgcgcactt cccggtagcg ctcgcacagc gccgtcccgg cgggggtggt ggagaacaac
     9121 acttccttgc cgatcttctc gctggccaca tggcccgccc gcaccagttt tttcaaggca
     9181 taggacacca cgtgcgtatc ttcgatattg agcacaaaac agatgtccgc cagttttttt
     9241 ttgcgaccca ggtgattgac atggtgcagc agggccacgt ccatcgctgt catgtctttt
     9301 tcgccggcgg cggccatgca gcgcaccatc cagcggttaa aggcattgct gaccaccatc
     9361 aggccaaatt caaactccga cagttcagcg ctgcgctcgg acaccaggtg tgacgaggag
     9421 acaatattca gcgaggggct catggcggcg ggggagtgtc aacgagaatg cgcaagtata
     9481 gcaatctcaa ctggcccgta aacttgccgg gcgcagcatt tttgcggcaa tatgccaata
     9541 tcaattcttc ttgcttctgc acaggagtcc ggcatgagcc aagccgctcc gcattactat
     9601 tctctgggcg aatccgccgt ggcgctggcc ctgccggcgc cggccaccct ggcgcaacag
     9661 cggcgcatct ggcggctggc cgccagcctg gccggcgcgc cgggcgtgct ggacatcgtc
     9721 cccggcatga acaacctcac cgtcagctat cacccgctgg attttgaccc ggaccacgcc
     9781 tttgactgcc tgcgccagca ctgggcggcc ttgggcgcgg ccgacgacga cgcgccagcg
     9841 cggcaggtgg acatcccggt gtgctacggc ggcgaggccg gccctgatct ggccgaggtg
     9901 gcgcgccaca ccggcctgac gccggccgag gtggtcaaac aacactgcgc ggcggaatat
     9961 caggtgttct tcatgggttt tcagcccggc tttgcctact tgggcggtct gcccgaggcg
    10021 ctggccaccc cccggcgcgc gcagccgcgc acccgcgtgc cggccggctc ggtgggcatt
    10081 ggcggcagcc agaccggcgt gtacccggcc agcagccccg gcggctggca gctgattggc
    10141 cacacccggc tcaaactgtt cgaccccacc gccacccagc ccaccctgtt gcagccgggt
    10201 gatcgggtgc gttttgtctg cgaggagctg cgccatgctt gaaatcctcc gccccggcgt
    10261 acagaccagc gtgcaagatc gcggccggcg cggcttgcgc catctgggca ttgcccaggc
    10321 cggcgcgctg gacatgccgg cgctataccg cgccaacagc ctgctgggca acgacccgga
    10381 cgccgccggc ctggaaatca ccctgggccc ggtcagcgtg cgctttggcc gcgacgcctg
    10441 gctggccttg tctggcgcgc attttcatcc cgagcttaac ggccggccga ttcacaccgg
    10501 ccagcgggtg ttcgcccagg ccgggcacac gctgacgctg agcggctgcc agtacggcat
    10561 gcgcacctat ctggcggtgg atggcggcat cgacgcgccg ctgacgctgg gcgcgcgcgc
    10621 caccgatctg caaggcggct ttggcggcgt gcatggccgc gcgctggaag ccggcgaccg
    10681 gctgccgctg ggcgcgccgc gcacgctgcg cgacggcccc ggctgccgca gcccggactg
    10741 gacgccctat gtgcgcgccc tgcccggccc ggattacgcc agcttcagcc ccagcgccca
    10801 ggcggcgttc tggcagcgac aatggacggt gacgcccagc agcaaccgga tgggcatgcg
    10861 cctgcgcggc acgccgctgg tgcgccgcca caatatggat ttgctctcgc atgcggtgtt
    10921 tcccggcgtg gtgcaggcgc cgccggatgg ccagcccatc atcctgctgg ccgacgcaca
    10981 gaccaccggc ggctatccgt gtttggcgac ggtgattcag gccgacctgt ggaagctggc
    11041 gcaagcccgc cccggcgcac acctgcattt tgtgccctgc tcggccg
//
LOCUS       DAOPYX010000063        11026 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_6810_length_11026_cov_9.207821, whole genome shotgun sequence.
ACCESSION   DAOPYX010000063 DAOPYX010000000
VERSION     DAOPYX010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 11026)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11026)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11026
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6810_length_11026_cov_9.207821"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1800
                     /gene="nirB"
                     /locus_tag="PKN55_04860"
     CDS             <1..1800
                     /gene="nirB"
                     /locus_tag="PKN55_04860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018150515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008942 - nitrite reductase [NAD(P)H]
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0042128 - nitrate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase large subunit NirB"
                     /protein_id="HNX70606.1"
                     /translation="VKAVRFKDGEEIPADLVVMAAGIRPNATLAEEAGLYCNRGVVVN
                     DTMQTYDPRIYAVGECANHRGIAYGLVAPLYEQARVVANHLAGFGIAKYQGSVTSTKL
                     KVTGIDLFSAGEFMGGGSTEEILLSDPMGGVYKKLVIQDNTLVGACLYGDTGDGAWYF
                     QLIREGHDIHEMRDHLMFGENHLGDAGHAGQSKAATMADDAEVCGCNGIKKGTIVKAI
                     QEHGLTSVDDIKRCTKAASSCGSCAGLCEQILIATVGGAADLTPKTEKPICGCTDHNH
                     ASVRKAIRDAHLTSIPEVMKALEWRTPNGCATCRPALNYYLISTWPKEAKDDPQSRFI
                     NERAHANIQKDGTYSVVPQMKGGVTNAAELRRIADVADKYAIPMVKMTGGQRIDLLGV
                     KKEDLVNVWKDLGMNSGHAYGKSIRTVKTCVGQEFCRFGTQNSTQMGIELETMLANMW
                     SPHKVKLAVSGCPRNCAESGIKDVGIIAVDSGWELYIGGNGGIKTEVAEFFVKVSSSE
                     EVKEYTGAFLQLYREEAFYLERTVHYLHRVGMEYIQNKVLKDPAQRLALYERLLYSLE
                     GLPDPWAARIAGAQAHEFTPLAAPAAQAEKETV"
     gene            1800..2108
                     /gene="nirD"
                     /locus_tag="PKN55_04865"
     CDS             1800..2108
                     /gene="nirD"
                     /locus_tag="PKN55_04865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018150514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008942 - nitrite reductase [NAD(P)H]
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HNX70607.1"
                     /translation="MSSWQKICRIEDIPVLGSRVVKRAGGDIAIFRNDQDEIFAIHDK
                     CPHKGGPLSQGIVFGRTVACPLHSWQIDLATGEALAPDHGCAGRFELKVEQGEVYLAL
                     "
     gene            2486..5179
                     /locus_tag="PKN55_04870"
     CDS             2486..5179
                     /locus_tag="PKN55_04870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018150513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HNX70608.1"
                     /translation="MMTVKSTCCYCGTGCGVEIDVDHGQVLAVRGDPEHPANFGRLCT
                     KGLTLAHTLHADSRLAEPRLRPGRIGPGQAVSWDVALDTVSERLAAIIAEHGPESVAF
                     YLSGQMLTEDYYVYNKLARGLIGTPHVDTNSRLCMSSAVTGYKLALGADAPPCSYEDF
                     DHADCVLIAGSNMAYAHPVLFRRLEAARAANPKMKLIVVDPRRTDTAASADLHLAIQP
                     GTDVALFNGMLHALIWDDRLDRDFISAHTEGFAELRHAVREYTPKMAADICGISEEAL
                     LTAARWFGEAGAALSLWCMGLNQSAHGSDKNLALINLHLATGQIGRPGAGPFSLTGQP
                     NAMGGREVGGLATALSGHRDPANPQHRAEVAAIWGVDALPATPGKTAIPMFDAIAAGQ
                     IKALWIVCTNPAHSMPDANKVREALAACELVIVQDAYADTDSVAYADVLLPAAGWAEK
                     DGTVTNSERRISRVRRAVAAPGSARADWDIGIDAARRLAARLGRGAHLFPYAGPDEVF
                     AEHCLTTVGRDLDIGGLSYAVLEAEGPQQWPRPAGASQGAARLYTDHHFATDNGRARF
                     HVARHHGVASPISARYPFHLLTGRLRDQWHGMSRSGRVPALFAHTQEAVLSMNPGDMV
                     RRGLADGELVRVSNSHGDWVLPVSSNDAMLSGQLFVPMHWSSAFVGSAGSNALTAAKI
                     DRKSFQPELKHVAVRVDKIDLAWQCAAVSACGDPVRALTALRPLLAECRFASATLLDG
                     ERPAVRVRFAHEALPAADFLARLDAALGLEGEGLAQVFADPRRGIDKRAIWQDGCLRA
                     VRLAGECCALGWLGERIVSGGEMGELRAMVFAPQANPPGLTRRARMVCHCAKVDEAAI
                     YQALASGASPDSLRDTLGCGSGCGSCMPEVRRMAASVTKAA"
     gene            5237..6004
                     /gene="cobA"
                     /locus_tag="PKN55_04875"
     CDS             5237..6004
                     /gene="cobA"
                     /locus_tag="PKN55_04875"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014086680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HNX70609.1"
                     /translation="MMKTPGKIWLIGAGPGDVELLTLKAVRALGEAQVWLVDDLVNPE
                     IAQFALPGTRIIGVGKRGGCRSTPQGFINRLMARYARQGQVVARIKGGDAMLFGRGGE
                     EVAYLQERGLSVEVVNGLTSGLVGATRAGIPLTHRDYTRGVTFVTAHAQDHSEPDWAA
                     LAATGTTLVIYMGMSRLSSIQAGLRAAGLPAEMPAAVIERAASPDERVLVTSLGLLAR
                     EAQLGGYASPAVIVVGEVVALATVREAELRRCWARCA"
     gene            complement(6327..6479)
                     /locus_tag="PKN55_04880"
     CDS             complement(6327..6479)
                     /locus_tag="PKN55_04880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX70610.1"
                     /translation="MELAHIIRWRFRGANQAMAEMIHDGAWQAGLDVNNNAEVAWARL
                     FFYSPA"
     gene            6653..8314
                     /locus_tag="PKN55_04885"
     CDS             6653..8314
                     /locus_tag="PKN55_04885"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNX70611.1"
                     /translation="MAIPQRGGLGRFSLKTLFVIMIGMVMTAYLAFGLWTWKVMTQVE
                     VNGPEYHRVVLGKDLIADILPPPKYIIETYLVTLQLDRAQPTERAALIERLKVLKKEY
                     DERQTYWIATEFEPDLKQAMIKTTHQPAMRYYSVLFERFIPALQAGDDAARASALADM
                     DAAYQQHRAAVDDLVAKTNAFNTRVEKETADTIAEGQWGMLSIWLLALLAVSGFTVAL
                     ARYLLRVLGGDPRVAMEVVQAIASNDLHQPVKVQMGDTQSLLAAMAAMQSHLKSVIGE
                     VNAAAQELSSASQHLTDTSQQVSQSSSEQSEAVMSVAAAMEEVAIGIAHISDNAEGAH
                     RLASESHDRSVSGIELAQSASLRMAELQGTVEASSSKISALQANSELIRSAAETIRTI
                     ADQTNLLALNAAIEAARAGEAGRGFAVVADEVRKLAENTARATGEIDQVIQAIHVDMN
                     HAVETMGDGVNRVGEGVSVTRALGDSMQELASGADGVVSTVSEITMSLRQQTAAHEQV
                     AANVETIARMSEANTGEVKRIASAAQTLAQLAQRLQGAISVFRLN"
     gene            complement(8523..>11026)
                     /gene="alaS"
                     /locus_tag="PKN55_04890"
     CDS             complement(8523..>11026)
                     /gene="alaS"
                     /locus_tag="PKN55_04890"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014087865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HNX70612.1"
                     /translation="KDVFLGFDKRPYTRATTSQKCVRAGGKHNDLENVGYTARHHTFF
                     EMLGNFSFGDYFKRDAISYAWEFLTSPQWLGLPKDKLMVTVYASDDEAFDIWNKEVGV
                     PADKIVRIGDNKGAPYASDNFWQMGDTGPCGPCTEIFYDHGAHIWGGPPGSPEEDGDR
                     FIEIWNNVFMQFNRTEDGVLHPLPKPSVDTGMGLERISAVLQHVHANYEIDLFVNLLK
                     AAARATGVEYNQQTPSLKVIADHIRACSFLIADGVLPSNDGRGYVLRRIIRRAIRHGY
                     KLGQKGLFFHTLVADLAAEMGDAYPELRQQQAKIEDALKQEEIKFAETLDKGMALLEG
                     ALAGEQKTLDGKVAFQLYDTYGFPLDLTADICRERDIAIDQAGFDAAMDAQRAQSRAA
                     SSFKMGGQLEYSGADTRFEGYTLATTQAHVLALYKDGQPVNSLQAGEAGVVVLDNTAF
                     YAEGGGQVGDVGEIAATGGLDALFDVKDTQKITASVFGHHGALSRGSLKVGDSVTATI
                     DLHARRATQRNHSATHLLHSALRHVLGSHVQQKGSLVNPERARFDFAHGQPVSAAELA
                     EVERLVNQAILANHEVKAELMNQDDALKAGAMALFGEKYGDVVRVLSMGDVSIELCGG
                     THVGRTGDIGLFKVVSEGGVAAGVRRIEAVTGEGAIAHIQTQDALLRELSATLKAQSL
                     DDVAGKIAAMQDGVKTLEKELARLKAKLAASAGDELAAQAQDIGGAKLLAAELEGADA
                     AALRETLDKLKDKLKSAIIVLASRGDGKVQLAAGVTADLTGKVKAGELVNFVAGQVGG
                     KGGGKPDMAMAGGSEPAKLPEALASVAGWVGGKLG"
BASE COUNT         1956 a         3585 c         3542 g         1943 t
ORIGIN      
        1 gtcaaggccg tgcgctttaa agacggcgaa gaaatccccg ccgacctggt ggtgatggct
       61 gccggcatcc gccccaacgc cacgctggcc gaagaagccg gcctgtattg caaccgtggc
      121 gtggtggtca acgacaccat gcaaacctac gacccgcgca tctacgccgt gggcgaatgc
      181 gccaaccacc gtggcattgc ctacggcctg gtggcgccgc tgtacgaaca ggcgcgcgtg
      241 gtggccaatc acctggccgg ttttggcatc gccaagtatc aaggctcggt gacttccacc
      301 aagctgaaag tcaccggcat tgatctgttc tccgctggcg aattcatggg cggtggcagc
      361 accgaagaaa tcctgctctc cgacccgatg ggcggcgtgt acaaaaagct ggtgattcag
      421 gacaacacac tggtgggcgc ctgcctgtac ggcgacactg gcgacggcgc ctggtatttc
      481 cagctgatcc gcgaaggcca cgacatccac gagatgcgcg accacttgat gtttggcgaa
      541 aaccacctgg gcgacgccgg ccacgccggg caaagcaagg cggccaccat ggccgacgac
      601 gccgaggtgt gcggctgcaa cggcatcaag aagggaacca tcgtcaaggc catccaagag
      661 cacggcctga ccagcgtgga cgacatcaag cgctgcacca aggccgccag ctcctgcggc
      721 tcctgcgccg gcttgtgcga gcaaatcctg attgccaccg tcggcggcgc cgccgacctg
      781 acgccgaaaa ccgaaaaacc gatttgcggc tgcaccgacc acaaccacgc cagcgtgcgc
      841 aaagccatcc gcgatgccca cctgaccagc attcccgagg tgatgaaagc cctggaatgg
      901 cgcaccccca acggctgcgc cacctgccgc ccggcgctga attattacct gatctccacc
      961 tggccgaaag aagccaagga cgatccgcaa agccgcttca tcaacgagcg cgcccacgcc
     1021 aatattcaaa aagacggcac ctattcggtg gtgccgcaga tgaagggcgg cgtcaccaat
     1081 gccgccgagc tgcgccgcat tgccgatgtg gccgacaagt acgccatccc gatggtgaaa
     1141 atgaccggcg gccagcgcat tgacttgctg ggcgtgaaaa aagaagacct ggtcaatgtc
     1201 tggaaagacc tgggcatgaa ctccggccac gcctacggca agtcgatccg cacggtgaaa
     1261 acctgcgtcg gccaggaatt ctgccgcttt ggcacgcaaa acagcacgca gatgggcatc
     1321 gagctggaaa ccatgctggc caatatgtgg agcccgcaca aggtcaagct ggcggtgtct
     1381 ggctgcccgc gcaactgcgc cgaatccggc atcaaggacg tgggcatcat cgcggtggat
     1441 tctggctggg agctctatat cggcggcaac ggcggcatca agaccgaagt ggcggaattc
     1501 ttcgtcaagg tgtccagctc ggaagaagtc aaggaataca ccggcgcctt cctgcaactg
     1561 taccgcgaag aggcgtttta tctggagcgc accgtgcact acctgcaccg cgtgggcatg
     1621 gaatacatcc agaacaaagt gctgaaagac ccggcccagc gtctggcgct gtacgagcgc
     1681 ctgctgtact cgctggaagg cctgcccgac ccgtgggcgg cgcgcattgc cggcgcccag
     1741 gcgcatgaat tcaccccgct ggcagccccg gccgcacaag ccgaaaagga gaccgtgtaa
     1801 tgagcagctg gcaaaaaatc tgccgcattg aagatattcc cgtgctgggc agccgggtgg
     1861 tcaaacgcgc gggtggcgac atcgccatct ttcgcaacga ccaggacgaa atcttcgcca
     1921 tccacgacaa atgcccgcac aaaggcggcc cgctgtcgca aggcattgtg tttggccgca
     1981 ccgtcgcctg tccgctgcac agctggcaga tcgacctggc cacgggcgag gcgctggcgc
     2041 cagaccacgg ctgcgccggc cgctttgagc tgaaagtgga gcaaggcgaa gtctatctcg
     2101 ccctgtaaga gccgcccaca aaacccgcct ggcgttgttg tgtgaccttg ccgtactacc
     2161 ccgtactgtc tgcggtcaca cgcctagcca ggaccgcttc gctgggtttt gtgagcgact
     2221 ctaaataaag cctgtttgag ccggcgtctt gccggcctgc tctgccggca gcgccggccc
     2281 gccacggcgg gcggcgcgca gtcagcagag atgggcgcag ccttggctgc atcctgaggt
     2341 ggtctgcgca tggtctgtcc ccccctctcc agaccttgcc cgcctgtgcg gttcccctgc
     2401 cgcattgccg cccggatgaa cccctcggcc aattgctgcg cccttctctg ccgtttgccc
     2461 ggccctgttt gataaggatg tcatcatgat gactgtcaaa tccacctgtt gctactgcgg
     2521 caccggctgt ggggttgaaa tcgacgtaga ccacggccag gtgctggccg tgcgcggcga
     2581 ccccgagcac ccggccaatt ttggccggct gtgcaccaag ggcctgaccc tggcgcacac
     2641 cctgcacgcc gattcgcggc tggccgagcc gcgcctgcgc cctggccgga ttggccccgg
     2701 ccaggcggtc agctgggacg tggcgctgga tacggtcagc gagcggctgg cggccatcat
     2761 cgccgagcac ggcccggaat cggtggcgtt ttatttgtct ggccagatgc tcaccgaaga
     2821 ctattacgtc tacaacaagc tggcgcgcgg cctgattggc accccgcatg tggacaccaa
     2881 ctcgcggctg tgcatgtcca gcgccgtcac cggctacaag ctggcgctgg gcgccgacgc
     2941 gccgccgtgc agctacgaag actttgacca cgccgattgc gtgctgattg ccggctcgaa
     3001 catggcctac gcccacccgg tgctgtttcg ccggctggaa gccgcccgcg ccgccaaccc
     3061 caagatgaag ttgattgtgg tcgatccgcg ccgcaccgac accgccgcca gcgccgacct
     3121 gcacctggcc atccagccgg gcaccgatgt ggcgctgttc aacggcatgc tgcacgcgct
     3181 gatctgggac gaccggctgg accgcgactt tatcagcgcg cacaccgaag gctttgccga
     3241 gctgcgccac gccgtgcgcg aatacacccc aaaaatggcg gctgatattt gcggcatcag
     3301 cgaagaagcc ctgctcaccg ccgcccgctg gtttggcgag gccggcgcgg cgctgtcgct
     3361 gtggtgcatg ggcctgaatc aatcggcgca tggctctgac aaaaacctgg ccttgatcaa
     3421 tctgcacctg gccaccggcc agattggccg ccccggcgcc gggccgtttt cgctgaccgg
     3481 ccagcccaac gccatgggcg ggcgcgaagt cggcggcctg gccaccgcgc tgagcggcca
     3541 ccgcgacccg gccaatccgc agcaccgcgc cgaagtggct gccatctggg gcgtggacgc
     3601 gctgccggcc acgccgggca aaaccgccat cccgatgttt gacgccatcg ccgccggcca
     3661 gatcaaggcg ctgtggattg tctgcaccaa cccggcgcac tcgatgccgg acgccaacaa
     3721 ggtgcgcgaa gcgctggcgg cctgtgaatt ggtcatcgtg caggacgcct acgccgacac
     3781 cgacagcgtc gcctacgccg atgtgctgct gccggccgcc ggctgggcag aaaaagacgg
     3841 cacggtgacc aactccgagc ggcgcatctc gcgcgtgcgc cgcgccgtgg ccgcgccagg
     3901 cagcgcccgc gccgactggg acatcggcat cgacgccgcc cgccggctgg ccgcccgact
     3961 ggggcgcggc gcgcatttat tcccctacgc cggcccggat gaagtgtttg ccgagcattg
     4021 cctgaccacg gtcggccgcg acctggacat tggcggcttg tcgtatgccg tgctggaagc
     4081 cgaaggcccg cagcaatggc cgcgcccggc cggcgccagc cagggcgccg cccggctgta
     4141 caccgaccac cactttgcca ccgacaacgg ccgcgcccgc tttcatgtgg cgcgccacca
     4201 cggcgtggcc agcccgatca gcgcccgcta tccctttcat ttgctgaccg gccgcctgcg
     4261 cgaccaatgg cacggcatga gccgcagcgg ccgggtgccg gcgctgtttg cccacaccca
     4321 ggaagccgtg ctgagcatga accccggcga catggtgcgt cgtggcctgg ccgatggcga
     4381 gctggtgcgg gtgagcaata gccacggcga ttgggtgctg ccggtcagca gcaacgacgc
     4441 catgctgtcg ggccaattat ttgtgcccat gcactggtcg tcggcctttg tcggcagcgc
     4501 cggcagcaat gcgctgaccg ccgccaagat cgaccgcaaa tcgtttcagc ccgagctcaa
     4561 gcatgtggcg gtgcgggtgg acaagattga tctggcctgg caatgcgcgg cggtgtccgc
     4621 ctgtggcgac ccggtgcgcg cgctgactgc gctgcgcccg ctgctggccg aatgccgctt
     4681 tgccagcgcc accttgctgg acggcgagcg cccggcggtg cgcgtgcgct ttgcccacga
     4741 agcactgccg gcggcggatt ttctggcccg gctggacgcc gcgctggggc tggagggcga
     4801 aggcctggcg caggtgtttg ccgacccgcg tcgcggcatc gacaagcgcg ccatctggca
     4861 ggacggctgc ctgcgcgcgg tgcgcttggc cggcgaatgc tgcgcgctgg gctggctggg
     4921 cgagcgcatt gtcagcggcg gcgagatggg cgagctgcgc gccatggtgt tcgccccgca
     4981 ggccaacccg ccggggctga cccgccgcgc gcgcatggtc tgccattgcg ccaaggtgga
     5041 cgaagcggcc atttatcagg ccctggccag cggcgccagc ccggacagcc tgcgcgacac
     5101 cctgggctgc ggcagcggct gcggctcgtg catgccggaa gtgcggcgga tggcggcctc
     5161 ggtcaccaaa gctgcgtaaa ttttgcaaac agcctggccg gcccgacgcc gcgccctggg
     5221 cagtgtggag acgaccatga tgaaaacccc cggaaaaatc tggctgattg gcgccggccc
     5281 tggcgatgtg gaattgctga cgctgaaggc cgtgcgcgcg ctgggtgagg cgcaggtgtg
     5341 gctggtggat gatctggtca acccggaaat cgcccagttt gccctgcccg gcacgcgcat
     5401 catcggcgtg ggcaagcgcg gcggctgtcg ctccaccccg cagggtttta tcaatcgctt
     5461 gatggcgcgc tatgcccgac agggccaggt ggtggcgcgc atcaagggcg gcgacgccat
     5521 gctgtttggc cgtggcggcg aagaagtggc ctatttgcaa gagcgcggcc tgagcgtgga
     5581 agtggtcaac ggcctgacct ccggcctggt gggcgccacc cgcgccggca ttccgctcac
     5641 ccaccgcgat tacacccgag gcgtgacctt tgtcaccgcc cacgcgcaag accattccga
     5701 gccggattgg gcggcgctgg ccgccaccgg cacgacattg gtgatttata tgggcatgag
     5761 ccgcctgagc agcattcagg ccggcctgcg cgccgccggc ctgccggccg agatgccggc
     5821 ggcggtgatc gagcgcgccg cctcgcccga cgagcgggtg ctggtcacct cgctgggcct
     5881 gctggcgcgc gaggcgcagc tgggcggcta cgccagcccg gcggtgattg tggtgggcga
     5941 agtggtggcg ctggccacgg tgcgcgaggc tgagttgcga cgatgctggg cgcgctgcgc
     6001 ctgatggcga tgcggcattg caatatgcaa aaacaattat ccgtgaatgg gtgaatccgt
     6061 ttttgtctga attgctacaa tctggtgcat ggtttgcgcc aaattgtagc attcaggccg
     6121 atttgatttt ttgtttatct ggattttttg aatgaccggt gtgaagaatc accttgccag
     6181 taaatcgccc atgctgcatt gcatgatttt tatttgatcc gacttgatct tacgcttgct
     6241 tgaaatagcc tgtaagcgca tgttttgcct gtgtttgtat aaactgcaca ctggtgtaca
     6301 atacttttcc gctgagtcat gtgcactcaa gctggtgagt aaaaaaataa gcgcgcccac
     6361 gcaacttctg cgttgttgtt tacgtccagc ccagcttgcc atgcaccatc atggatcatt
     6421 tcggccatgg cctgatttgc tccacgaaaa cgccagcgga taatatgcgc caattccata
     6481 aaaaaatgtc ccaatatcgg tttatgcctg gacagacgct ttgtatgctc gtctgtttct
     6541 gtccgtctgg ccccgatgcc cctcagggag gccgccgagt cggccatggg cgggacaggt
     6601 ctgcagccgt gtgtctgcag gtctcaccaa aaaaccacag gagcatgaca tcatggcaat
     6661 ccctcaacgc ggcggattgg ggcgtttcag cctgaagacg ctgttcgtga tcatgatcgg
     6721 catggtcatg acggcatatc tggccttcgg gctgtggacc tggaaagtga tgacccaggt
     6781 ggaagtcaac gggccggaat accatcgggt ggtgctgggc aaagatttga ttgccgacat
     6841 cctgccgccg ccgaaataca tcatcgaaac ctatctggtg accttgcagc tggatcgcgc
     6901 ccagcccacc gagcgcgcgg cgctgatcga gcggctgaag gtcctgaaaa aagaatacga
     6961 cgagcggcaa acctactgga ttgccacaga attcgagcca gacctgaagc aggcgatgat
     7021 caagaccacc caccagccag ccatgcgcta ctacagcgtg ctgttcgagc gcttcatccc
     7081 ggccctgcag gccggtgacg acgccgcgcg cgccagcgcg ctggccgaca tggacgccgc
     7141 ctatcagcaa catcgcgcgg cggtggacga tctggtggcc aagaccaatg ccttcaacac
     7201 ccgggtggaa aaagaaaccg ccgacaccat cgccgagggg caatggggca tgctgagcat
     7261 ctggctgctg gccttgctgg cggtcagcgg ctttaccgtg gccttggccc gctatctgct
     7321 gcgcgtactg ggcggcgatc cgcgcgtggc catggaagtc gttcaggcca tcgccagcaa
     7381 cgatctgcat cagccggtga aagtgcaaat gggtgacacg cagtcgctgc tggcggccat
     7441 ggcggccatg caaagccacc tgaaaagcgt gattggcgaa gtcaacgccg ccgcgcaaga
     7501 gctctcgtcc gcctcgcagc acctgaccga cacctcgcag caagtgtcgc aaagctccag
     7561 cgagcaaagc gaagcggtga tgtcggtggc cgccgccatg gaagaagtcg ccatcggcat
     7621 cgcccacatt tctgacaacg ccgaaggtgc gcaccgcctg gccagcgaat cgcacgaccg
     7681 ctcggtcagc ggcattgagc tggcgcaaag cgcctcgctg cggatggctg agctgcaagg
     7741 cacggtagaa gcctcgtcga gcaagatttc tgccctgcag gccaattccg agctgattcg
     7801 cagcgcggcg gaaaccatcc gcaccattgc tgaccagacc aacctgttgg cgctgaacgc
     7861 cgccattgag gccgcgcgcg cgggcgaggc cgggcgcggc tttgccgtgg tggccgacga
     7921 agtacgcaag ctggcggaaa acaccgcccg cgccaccggc gaaattgatc aggtgatcca
     7981 ggccattcat gtggacatga atcacgcggt ggaaaccatg ggcgatggcg tcaaccgggt
     8041 gggcgaaggc gtcagcgtca cccgtgcgct gggcgactcc atgcaggagc tggccagcgg
     8101 cgccgatggc gtggtcagca ccgtgtcgga aatcaccatg tcgctgcgcc agcaaaccgc
     8161 cgcgcacgag caagtggcgg ccaatgtgga aaccatcgcc cgcatgagcg aggccaatac
     8221 cggcgaggtc aagcgcatcg ccagcgccgc gcaaaccctg gcacagctgg cgcagcgcct
     8281 gcaaggggcg atcagcgtat tccggctgaa ctgagcaaaa acgcgcctga cgccgccagc
     8341 gggcgtgtgc ccctggcggc ttttttctgg cggcaaatgc ccggccccgg atcatgccca
     8401 gtcgccagat tacctcagca acagactgat ccgttgtgcg attctggccc aagcacactg
     8461 acagggcaca aaaaaacccc caccagccag gctgatgggg gttttttggc gcgaggggga
     8521 gatcagccca gcttgccgcc cacccagccg gccacgctgg ccagcgcttc cggcagcttg
     8581 gccggttcgc tgccgccggc catggccatg tccggcttgc cgccgccctt gccgccgacc
     8641 tggccggcga caaagttgac cagctcgcca gccttgacct tgccggtcag gtcggcggtg
     8701 acgccagcgg ccagctgcac cttgccgtcg ccccggctgg ccagcacgat gatggccgac
     8761 ttgagcttgt ctttcagctt gtccagcgtt tcgcgcagcg cggcggcgtc ggcgccttcc
     8821 agctcggcgg ccaacagctt ggcgccgccg atgtcctgcg cctgggcggc cagttcgtcg
     8881 ccggcgctgg cggccagttt ggctttcagg cgggccagtt ctttttccag cgttttcacg
     8941 ccgtcctgca tggcggcgat cttgccggcc acgtcgtcca ggctttgcgc cttcagcgtg
     9001 gcggacagct cgcgcagcag ggcgtcttgc gtctggatgt gggcaatcgc gccttcgccg
     9061 gtcaccgctt caatccggcg cacgccagcg gccacgccgc cttcgctgac cactttgaac
     9121 aggccgatgt cgccagtgcg gcccacatgg gtgccgccgc acagctcgat ggacacgtcg
     9181 cccatgctca gcacacgcac cacatcgccg tatttttcgc caaacagggc catggcgccg
     9241 gctttcagcg cgtcgtcctg gttcatcagc tcggccttga cttcgtggtt ggccagaatc
     9301 gcctggttga ccagacgctc cacctcggcc agttcggccg cgctcaccgg ctggccgtgg
     9361 gcaaagtcaa agcgcgcgcg ttccgggttc accagcgagc ccttttgctg cacatggctg
     9421 cccagcacat ggcgcagcgc gctgtgcagc aagtgggtgg ccgagtggtt gcgctgggtg
     9481 gcgcgacggg cgtgcaggtc gatggtggcg gtgacgctgt cgcccacttt caggctgccg
     9541 cgcgacagcg cgccgtggtg gccgaacacg ctggcggtga ttttttgggt gtccttgacg
     9601 tcaaacagcg cgtccaggcc gccggtggcg gcaatttcgc ccacatcgcc cacctggccg
     9661 ccgccttcgg cgtaaaacgc ggtgttgtcc agcaccacca cgccagcctc gccggcttgc
     9721 aggctgttca ccggctggcc gtctttgtac aaggccagca catgcgcctg cgtggtggcc
     9781 agggtgtagc cctcaaaacg ggtgtcggcg ccgctgtatt ccagctggcc gcccatcttg
     9841 aagctggagg cggcgcggct ttgcgcgcgc tgggcgtcca tggcggcgtc aaagccggcc
     9901 tggtcgatgg cgatgtcgcg ctcacggcag atgtcggcgg tgaggtcgag cgggaagcca
     9961 taggtgtcgt acagctggaa ggccaccttg ccgtccaggg ttttttgctc gccggccagc
    10021 gcgccttcca agagcgccat gcccttgtcc agggtttcgg cgaacttgat ttcttcctgc
    10081 ttgagcgcgt cttcgatctt ggcctgttgc tggcgcagtt ccgggtaggc gtcgcccatt
    10141 tcagcggcca gatcagccac cagggtgtgg aagaacaagc ccttctggcc cagcttgtag
    10201 ccgtggcgga tggcgcggcg gatgatgcgg cgcagcacat agccgcgacc gtcgttggac
    10261 ggcagcacgc cgtcggcaat caggaaggaa cacgcgcgga tatggtcggc gatgactttc
    10321 agcgacggcg tttgctggtt gtattccacg ccagtggcgc gcgcggcggc cttcagcaga
    10381 ttgacgaaca ggtcgatttc gtaattggcg tgcacatgtt gcagcaccgc cgaaatccgc
    10441 tccaggccca tgccggtgtc cacgctgggc ttgggcagcg gatgcagcac accgtcttcg
    10501 gtgcggttga actgcatgaa cacgttgttc cagatttcaa taaagcggtc gccgtcttct
    10561 tccgggctgc cgggcgggcc gccccagatg tgggcgccgt gatcgtaaaa gatctcggtg
    10621 cacgggccac acgggccggt gtcgcccatc tgccagaagt tgtccgaggc atacggcgcg
    10681 cccttgttgt cgccaatgcg cacaatcttg tccgccggca cgccgacttc tttgttccag
    10741 atgtcaaacg cctcgtcatc gctggcgtag accgtcacca tcagcttgtc tttgggcagg
    10801 cccagccatt gcgggctggt gagaaactcc caggcgtagc tgatggcgtc gcgcttgaag
    10861 tagtcgccaa agctgaaatt gcccagcatt tcaaagaagg tatggtggcg ggcggtgtaa
    10921 ccgacgtttt ccaggtcgtt gtgcttgccg ccggcgcgca cgcatttctg gctggtggtg
    10981 gcgcgggtgt acgggcgctt gtcaaaaccc aggaacacat ccttga
//
LOCUS       DAOPYX010000064        10839 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_6972_length_10839_cov_10.060274, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000064 DAOPYX010000000
VERSION     DAOPYX010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 10839)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10839)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10839
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6972_length_10839_cov_10.060274"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            199..1878
                     /locus_tag="PKN55_04895"
     CDS             199..1878
                     /locus_tag="PKN55_04895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008953023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome D1 domain-containing protein"
                     /protein_id="HNX70613.1"
                     /translation="MPLLAAASLATAADGKSVSQTDMAYQAGGSPLAKEAMHQNINPK
                     APPMTAAEFERAKQIYFERCAGCHGVLRKGATGKALTPDITLGKGTDYLKVFIAYGSP
                     AGMPNWQTSGEMSEQDVDLMARYIQQEPPTPPEFSLADIEKTRKIYVPVEQRPTRKMN
                     HYNLDNLFSVTLRDSGEVALIDGDTKQIINIVKTGYAVHISRLSKSGRYLYVIGRDAR
                     LNLIDLWMAKPDNVAEIKVGLEARSVETSKFKGFEDKIAVAGTYWPPQFVIMDGDTLK
                     PRKVVSTRGMTVDNEYHPEPRVASIVASHYRPEFVINAKETGKIMMVDYSDLNNLKTT
                     TIDSAKFLHDGGFDSTGRYFLVAANASNKIAVVDTKEDKLAALVDVGKTPHPGRGANF
                     THPKYGPVWATSHLGAEDVTLIGTDPVKHKPYAWKAVEVLKGQGGGSLFIKTHPKSSN
                     LWVDTPLNPDAKMSQSVAVFDIKNLNKPPQIVDVAACAQLGDGAKRVVQPEYNKAGTE
                     VWFSVWSAKDKESAIVVVDDKTRQCKQVIKDKRLITPTGKFNVYNTQHDVY"
     gene            1926..2231
                     /locus_tag="PKN55_04900"
     CDS             1926..2231
                     /locus_tag="PKN55_04900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012695527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HNX70614.1"
                     /translation="MRIFSLSLLAALASTASLPALASAELAKQHNCLSCHSVDKKVVG
                     PSYKDIAAKYKGQSVEAMLIDKIKHGSKGVYGPIPMPANANVPDADVKTLVKWILAQ"
     gene            2228..2548
                     /locus_tag="PKN55_04905"
     CDS             2228..2548
                     /locus_tag="PKN55_04905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018151792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HNX70615.1"
                     /translation="MNWRALAVLGLSLASAAHAAEPPSPARAAALSHMLHQECGACHG
                     LRLTGGLGSPLTAAALADKPADSLTATILDGRPGSAMPPWRPFVSADEARWLVEQLQQ
                     GRLP"
     gene            2545..3708
                     /locus_tag="PKN55_04910"
     CDS             2545..3708
                     /locus_tag="PKN55_04910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018151791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome D1 domain-containing protein"
                     /protein_id="HNX70616.1"
                     /translation="MIARLLSALSAALCLLLAACASPPLRGTGDLGVVIERARGSVQI
                     VEQSARRSLGRVDGLGDLSHASVVFSRDGRYAYVFGRDGGLSQVDVLSQRLVKRVMQA
                     GNSIGGAISSDGRIVAAQNYQPGGVKLFDADTLELLAEVSTDGSKVVGLADLPGNRFA
                     FSLFEAGEIWLIDASQPRAPQVTRYPNIGRQPYDGLASQDGRHYLAGLYGEDGLALLD
                     TWQPEQGVRRILNGYGRGRAALPVYKMPHLRGWSLAGDYAFLPAIGHHQVLIADRRSW
                     QEVARVAVHGQPVFVMAEPGGRRVWVNFAFPHNDTVQVIDVPSRQIVATLKPGRAILH
                     MEFTARGDAVWLSSRDDNRLTVIDTATLNTVAELDAEQPSGIFFTWRAQRMGM"
     gene            3740..4717
                     /locus_tag="PKN55_04915"
     CDS             3740..4717
                     /locus_tag="PKN55_04915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017507124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HNX70617.1"
                     /translation="MNAPISHPLPLALLDRYQRHFPLCPRPYAEVAGHDGWDEGELLA
                     ALRAAAEAGVLSRIGAVFAPNTVAASTLAALAVPPDQLAAVAAQVSARREVSHNYQRS
                     HAYNLWFVATAADRAALDALLAGIAADSGLPVLDVPLEREYHIDLGFALHGEAAARTP
                     LPPSPGLLTLSAAQRRLLAALGPGLALTPRPYLALAQACELSEAEVLATLAHWQAIGA
                     LRRFGLVLRHHELGYRANVMCVWQIPPAARDAIGAQLAQDPAVTLCYARPARPPHWPY
                     TLFAMVHGKSRAEVDAAIDRLIAQHALAAWPHARLYTLHRYAQRGPHYA"
     gene            4710..5702
                     /locus_tag="PKN55_04920"
     CDS             4710..5702
                     /locus_tag="PKN55_04920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014086636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HNX70618.1"
                     /translation="MLEPHAQPLNPLDQAIIDRLQGDFPLCAYPYDEAAAPLGIDADT
                     LMARLDTLLARRVLTRFGPMFQVERMGGAFVLAALAAPEARFAEVAEQVNALPEVAHN
                     YRREHTLNMWFVLATAHPDGIDAARQRIEAATGLTVRLFPKLREYYVGMRFTVGGRAP
                     VGGEVIHRPSHAPATPLSAADTALVRACQAGLPRCRRPYQALADSLGWQEAQVIERLQ
                     HMLAHGIIRRIGAAPNHYAIGYTANGMAVFDIDDAHIDACGERLGALAQVSHCYHRPR
                     QPGWPYNLFAMCHGDHRDAVRADVALLREQLGSACRGHDVLFSTAMLKKSGVRI"
     gene            complement(5833..6153)
                     /locus_tag="PKN55_04925"
     CDS             complement(5833..6153)
                     /locus_tag="PKN55_04925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019921412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNX70619.1"
                     /translation="MNVIHTAEQLFLSLKQLPSAERDKFFVLLGSKAFADDALSHDEV
                     FGHLRDEEFTAAEAAEYLEVSIATFRRYVQAGKITAHSSVGNAHLYPLAALRALKKAI
                     KLAK"
     gene            complement(6150..6509)
                     /locus_tag="PKN55_04930"
     CDS             complement(6150..6509)
                     /locus_tag="PKN55_04930"
                     /inference="COORDINATES: protein motif:HMM:NF027117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HNX70620.1"
                     /translation="MLLTPRYKRPFATFVKKQHKPFQAVIEDEVLEVCANPAMGEAKV
                     GDLEGILVHKFSYKKQLYLMAYHLPETHDATPKADEDDAEQEGPQFLFMDFYQIGSHE
                     NFYDDLKAYLRADGWYK"
     gene            complement(6592..>6905)
                     /locus_tag="PKN55_04935"
     CDS             complement(6592..>6905)
                     /locus_tag="PKN55_04935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX70621.1"
                     /translation="LLAWALITPLTSWADTPAWRIHTENYARPPYSGAVYYFYHAGPK
                     IVCTKLSVCNKYDQCEVEYRQGEYREDGDDEPASRTDAVPIPPAKLKAHKCLVKFGLV
                     K"
     assembly_gap    6906..7005
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            7082..8314
                     /gene="nirJ"
                     /locus_tag="PKN55_04940"
     CDS             7082..8314
                     /gene="nirJ"
                     /locus_tag="PKN55_04940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014086639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme d1 biosynthesis radical SAM protein NirJ"
                     /protein_id="HNX70622.1"
                     /translation="MAALILLRAFLMLRLTPYLASLLHPTPLAPARRPAGPVVIWNLI
                     RRCNLTCQHCYSISADRDFPGELSTSEVFAVMDDLRGFGVPVLILSGGEPLLRADIFD
                     IAARAKTLGFYVGLSSNGTLIDAGMAARLAGLGLDYLGVSLDGMAATHDRFRRLEGGF
                     ARAAAGIRLARDAGMKVGVRYTLTEDNAADLPALLQWVDDEGIDKFYFSHLNYAGRGN
                     KHRDSDARHARTRWAMDLLIEACLRHLRAGQAKDFVTGNNDADAVYLLRWAQTHYPEQ
                     AEALRQRLLAWGGNASGVNVANIDNLGNVHPDTMWWDIQLGNVRERPFSQIWPDVSHP
                     LMAGLKQRPRPVHGRCGACPHLAICNGNTRVRAWALTGDFWAADPACYLSDAELGLPP
                     GKYAQAELTAWQRQGATA"
     gene            8311..9846
                     /locus_tag="PKN55_04945"
     CDS             8311..9846
                     /locus_tag="PKN55_04945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008953012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome D1 domain-containing protein"
                     /protein_id="HNX70623.1"
                     /translation="MRRAWLWMGLALAASGHAAAPAALYQQHCQSCHGEQRLGGMAPA
                     LLPQSLERSKPAELQATVRDGRPATQMQGFADRLSADEIQALVGWLRTPAATPPRWSE
                     AEMAASHRQFVPLAQLPDAPGHGADPANLFVVVEAGDHHVSVLDGERFTVLARFASHY
                     ALHGGPKFSPDGRFVYFASRDGWVSQYDLYSLQKVAEIRVGLNTRNVAASADGRWLLA
                     GNTLPENLVLLDARTLQPVKTLPVRTQQGQLSRVSAVYDAAPRQSFIVALKDAPELWE
                     ISYNPAAEPIYDGYVHDYAMGEGLAKPGFLNPRRTPLDAVLDDFFFDPDYRHVIGASR
                     DGRAQVVNLDIRRQVAALQLDGMPHLGSGITFKRDGRTLLATPNLKTGQVSVIDTGNW
                     QEVTRIATPGPGFFLRSHERTPYAWVDAMMSAQRDTLTLIDKRTLQVAHTLRPRPGKT
                     TAHVEFTRDGRYALVSVMETPGELLVIDAATLREVAALPMMKPIGKYNVHNKITRSEG
                     TSH"
     gene            9962..10645
                     /locus_tag="PKN55_04950"
     CDS             9962..10645
                     /locus_tag="PKN55_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017507128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HNX70624.1"
                     /translation="MSQIDVRALLRHQPMFHQLSDAQIDALAPGVREVRGQKSQVVFH
                     RGDLCEGLYLVVYGRVKLSISSAQGVEKVIEIIQPGQSFAEAVMFLGTPCPVTAQFLE
                     DGLLLQVEASAIDRAMADDPAFARRLLAGLSMRLHSLIRDVERYSIESSTQRVLGYLL
                     QYADPEQGAQQVVTLPVNKNLIASRLNLTPETFSRVLHQLSDAGLLAVNGRDITLLDL
                     DKVRAFGCG"
BASE COUNT         1905 a         3550 c         3452 g         1832 t
ORIGIN      
        1 cccacaccgg acactttccg ccacccaccc ccacaaagtt ccccgccccg cccgccagcc
       61 tgaaaaccgt tgattcgaat caatgatggc gctttgccgg ccagaggatg atgtccgcat
      121 tccttctttc gtagcgtggt ggagcagaca acaatgagac acaccctgaa ccccctggtc
      181 cgcagtctgg catgggcaat gccgttgctg gcggcggcgt cgctggcaac ggcggcggat
      241 ggcaaatcgg tcagccagac cgatatggcc tatcaggctg gcggctcgcc gctggccaaa
      301 gaggcgatgc accagaacat caacccgaaa gcgccgccga tgacggcggc cgagttcgag
      361 cgcgccaagc agatttactt tgagcgctgc gccggctgtc atggcgtgct gcgcaagggc
      421 gccaccggca aggcgctgac cccggacatc accctgggca agggcaccga ttacctgaaa
      481 gtgttcatcg cctacggcag cccggccggc atgcccaact ggcagacctc gggcgagatg
      541 agcgagcagg atgtggattt gatggcgcgc tacatccagc aagagccgcc caccccgccg
      601 gaattctcgc tggcggatat tgaaaaaacc cgcaagatct atgtgccggt ggaacaacgc
      661 cccacccgca agatgaacca ttacaacctg gacaatctgt tctcggtcac cctgcgcgac
      721 agcggcgagg tggcgctgat tgacggcgac accaaacaga tcatcaatat cgtcaaaacc
      781 ggctacgccg tgcatatctc gcggctgtcc aaatccggcc gctatttgta tgtgattggc
      841 cgcgacgcgc ggctgaacct gatcgatttg tggatggcca agccggacaa tgtggcggaa
      901 atcaaagtcg gcctggaagc gcgctcggtg gagacgtcca agttcaaggg ctttgaagac
      961 aagatcgccg tggccggcac ctactggccg ccgcagtttg tcatcatgga cggcgacacg
     1021 ctcaagccgc gcaaagtggt gtccacccgc ggcatgacgg tggataacga ataccacccg
     1081 gaaccgcgcg tggcctcgat tgtggccagc cattaccgcc cggagtttgt gatcaacgcc
     1141 aaggaaaccg gcaagatcat gatggtggat tactctgacc tcaacaacct gaaaaccacc
     1201 accatcgatt cggccaagtt cctgcacgat ggcggctttg acagcaccgg gcgttacttc
     1261 ctggtggcgg ccaatgcctc caacaagatc gccgtggtgg acaccaagga agacaaactg
     1321 gccgcgctgg tggacgtggg caaaaccccg caccccggcc gtggcgccaa cttcactcac
     1381 ccgaaatacg gcccggtgtg ggccaccagc cacctgggcg ccgaggacgt gacgctgatc
     1441 ggcaccgacc cggtcaagca caagccatac gcctggaagg ccgtcgaagt gctcaagggc
     1501 cagggcggcg gctcgctgtt tatcaagacc cacccgaaat ccagcaattt gtgggtggac
     1561 accccgctga acccggacgc caagatgagc cagagcgtgg cggtgtttga catcaaaaac
     1621 ctgaacaagc cgccgcagat tgtcgatgtg gccgcctgcg cccagctggg cgacggcgcc
     1681 aagcgcgtgg tgcagccgga atacaacaag gccggcaccg aagtgtggtt ctcggtgtgg
     1741 agcgccaagg acaaggaatc cgccatcgtg gtggtggacg acaaaacccg ccagtgcaag
     1801 caggtgatca aggacaagcg cctgattacc cccaccggca agttcaacgt ctacaacacc
     1861 cagcacgacg tgtattgacc tgaccgcgcc gccctggcgg gcggcgcgcc tgactggaga
     1921 gtgtcatgcg tattttttcc ctgtccttgc tggcggccct ggccagcacc gccagcctgc
     1981 cggcgctggc ctcggccgag ctggccaaac agcacaactg cctgtcctgc cacagcgtgg
     2041 acaaaaaagt ggtcggcccc tcgtataaag acatcgccgc caagtacaaa ggccaatcgg
     2101 tggaagccat gctgatcgac aaaatcaagc acggcagcaa gggcgtgtac ggccccatcc
     2161 cgatgccggc caacgccaat gtgccggatg ccgacgtcaa gaccctggtg aaatggattc
     2221 tggcgcaatg aactggcgcg cgctggcggt gttgggctta agcctggcca gcgccgccca
     2281 cgccgccgag ccgcccagcc cggcgcgcgc ggcggcgctc agccatatgc tgcatcagga
     2341 atgcggcgcc tgccatggcc tgcgcctgac cggcgggctg ggcagcccgc tgaccgccgc
     2401 cgcgctggcc gacaaaccgg ccgacagcct gacggccacc attctggacg gtcgccccgg
     2461 cagcgccatg ccgccgtggc gaccgtttgt cagcgccgac gaagcgcgct ggctggttga
     2521 acaactgcaa caaggacgcc tgccatgatc gcccgactgc tgtctgccct gagcgccgcg
     2581 ctctgcctgc tgctggccgc ctgcgccagc ccgccgctgc gcggaaccgg ggatttgggc
     2641 gtggtgatcg aacgcgcgcg cggcagcgtg cagattgtcg agcaaagcgc ccggcgcagc
     2701 ctgggccggg tggacggcct gggcgatttg tcacacgcct cggtggtgtt ctcgcgcgat
     2761 ggccgctacg cctatgtgtt tggccgcgat ggcggcttga gtcaggtgga cgtgctcagc
     2821 cagcggctgg tcaagcgggt gatgcaggcc ggcaacagca ttggcggggc gatttccagc
     2881 gatgggcgga ttgtcgccgc gcaaaattat cagcccggcg gggtcaagct gtttgacgcc
     2941 gacacgctgg agctgctggc cgaagtcagc accgacggct cgaaagtggt cggcctggcc
     3001 gacctgcccg gcaatcgctt tgccttcagc ctgtttgaag ccggcgaaat ctggctgatc
     3061 gacgccagcc aaccgcgcgc cccacaagtc acccgctacc cgaacatcgg ccgccagccc
     3121 tacgacggcc tggccagcca ggacggccgc cactatctgg ccggcttgta cggcgaagac
     3181 ggcctggcgc tgctggacac ctggcagccg gagcagggcg tgcgccgcat tctgaatggc
     3241 tacgggcgcg ggcgcgccgc gctgccggtg tacaaaatgc cgcacctgcg cggctggagc
     3301 ctggccggcg actacgcctt tttgccggcc atcggccacc accaggtgct gatcgccgac
     3361 cggcgcagct ggcaggaagt cgcccgcgtg gcggtgcacg gccagccggt gtttgtcatg
     3421 gccgaaccgg gcggccggcg ggtgtgggtg aactttgcct ttccgcataa cgacaccgtg
     3481 caggtgatcg acgtgcccag ccggcagatc gtcgccaccc tgaaacccgg ccgcgccatc
     3541 ctgcacatgg agttcaccgc ccgtggcgac gcggtgtggc tgtccagccg cgacgacaac
     3601 cgcctgaccg tgatcgacac cgccacgctg aacaccgtgg ccgaactgga cgccgaacaa
     3661 ccgtcgggga tttttttcac ctggcgcgcg caaaggatgg ggatgtgatg cgcgccaagc
     3721 catgccggag ccttgccgca tgaacgcgcc gataagccac cccctgccgc tggccttgct
     3781 ggaccgctat cagcgccact ttccgctgtg cccgcgcccg tatgccgagg tggccgggca
     3841 cgatggctgg gacgagggcg agttgctggc ggccttgcgc gcggcggctg aggccggggt
     3901 gctgtcgcgc attggcgcgg tgttcgcccc caataccgtg gcggccagca cgctggcggc
     3961 gctggccgtg ccgcccgacc aactggcggc cgtcgccgcg caggtcagcg cgcggcgcga
     4021 ggtcagccac aattaccagc gcagccacgc ctataacctg tggtttgtcg ccaccgccgc
     4081 cgaccgcgcc gcgctggatg cgctgctggc cggcattgcc gccgacagcg gcttgccggt
     4141 gctggatgtg ccgctggagc gggaatacca catcgacctg ggctttgccc tgcacggcga
     4201 agccgccgcc cgcacgccgc tgccgccctc gcccggcctg ctgacgctca gcgcggcgca
     4261 acgccgcttg ctggcggcgc tgggcccagg cctggcgctg acgccgcgcc cctatctggc
     4321 gctggcgcaa gcgtgcgagc tgagcgaagc cgaggtgctg gcgaccttgg cgcactggca
     4381 ggcgattggc gcgctgcgcc gctttggcct ggtgctgcgc caccacgagc tgggctatcg
     4441 ggccaatgtg atgtgcgtgt ggcaaatccc gcccgccgcc cgcgacgcca tcggcgccca
     4501 gctggcgcaa gacccggcgg tgacgctgtg ttacgcccgc ccggcgcgac cgccgcactg
     4561 gccctacacg ctgtttgcca tggtgcacgg caaaagccgc gccgaggtgg acgccgccat
     4621 cgaccgcctg attgcccagc acgcgctggc cgcctggccg cacgcgcggc tgtacaccct
     4681 gcaccgctac gcccagcgag gcccgcacta tgcttgaacc gcacgcccaa cccctcaacc
     4741 cgctggacca ggccatcatc gaccgcctgc aaggggattt tccgctgtgc gcttatccct
     4801 atgatgaagc cgccgcgccg ctgggcatcg acgccgacac gctgatggcg cggctggaca
     4861 cgctgctggc gcgccgggtg ctcacccgct ttggcccgat gtttcaagta gaacgcatgg
     4921 gcggcgcctt tgtgctggcg gcgctggccg cgcccgaggc gcgctttgcc gaggtggccg
     4981 agcaggtcaa cgccctgccg gaagtcgccc acaactaccg gcgcgaacac acgctgaata
     5041 tgtggtttgt gctggccacc gcccaccccg acggcatcga cgccgcccgc cagcgcatcg
     5101 aagccgccac cggcctgacg gtgcgcctgt ttcccaaact gcgcgaatat tatgtgggga
     5161 tgcgctttac cgttggtggc cgcgcgcccg tgggcggcga agtgatccac cgccccagcc
     5221 acgccccggc aacgccgctg tccgccgccg acaccgcgct ggtgcgcgcc tgccaggccg
     5281 gcctgccgcg ctgccgccgc ccctatcagg cgctggccga cagcctgggc tggcaagaag
     5341 cgcaggtgat cgaacgcctg caacacatgc tggcgcacgg catcatccgc cgcatcggcg
     5401 ccgcgcccaa ccactacgcc atcggctaca ccgccaacgg catggcggtg ttcgacatcg
     5461 acgacgccca catcgacgcc tgcggcgaac gcctgggcgc gctggcgcaa gtcagccact
     5521 gctaccaccg cccacgccaa ccaggctggc cgtataacct gttcgccatg tgccacggcg
     5581 accaccgcga cgccgtgcgc gccgacgtcg ccctgctgcg cgaacaactg ggctcagcct
     5641 gccgggggca cgatgtgctg ttttcgacgg cgatgctgaa aaaaagtggg gtgcggattt
     5701 agggtgggag agcgagcatg gtgggcgggg tgactttgct tgagtccgta gccacagacg
     5761 cacatatcgc cggataaatt gaacctgcac gccgcccagg ctcaggccgc catcatgccc
     5821 tcggccaagc ggctacttgg ccagtttgat ggcttttttt aacgcccgca gcgccgccag
     5881 cgggtaaaga tgagcattcc ccacgctgga atgcgcggtg attttcccgg cttgcacata
     5941 acggcgaaac gtggcgatgg agacctccag atattccgcc gcttcggcgg cggtgaattc
     6001 ttcatcgcgc aaatggccga acacctcgtc gtggctgagg gcatcatcgg caaacgcctt
     6061 gctgcccaac aagacaaaga acttgtcccg ctcggcggac ggcagttgtt tgagactgag
     6121 aaaaagctgc tcggcggtgt gaatgacgtt catttgtacc atccatctgc tctcaaatag
     6181 gctttcaggt cgtcgtagaa attctcgtgc gaccctattt gataaaagtc catgaacaag
     6241 aactgtggcc cctcctgctc ggcgtcatct tcatccgcct tgggtgttgc gtcatgcgtc
     6301 tccggaaggt ggtaggccat caaatacagc tgcttcttgt agctgaactt gtgcaccaga
     6361 atgccttcca ggtcgccgac cttggcttct cccatggctg gattggcgca tacctccagc
     6421 acctcatcct caatcaccgc ctgaaaaggc ttgtgctgct ttttcacaaa cgtggcaaat
     6481 ggtcttttgt agcgaggggt gagcagcatg gccgattcct ttctgtatca attttgatat
     6541 agaaaggaat caagcgcaag tttctgtgtc attgcttttg ccacgcgccg ctcacttcac
     6601 caacccaaac ttcaccaaac acttatgcgc cttcagcttg gccggcggga tgggaacagc
     6661 gtcggtgcgg ctggcgggtt cgtcatcgcc gtcttcgcgg tattcgccct ggcgatattc
     6721 cacctcgcac tggtcgtatt tattgcacac gctgagcttg gtgcacacga tcttggggcc
     6781 ggcgtggtag aagtaataaa cggcgccgga gtagggcggg cgggcgtagt tttcggtgtg
     6841 gatgcgccag gcgggggtgt cggcccagct ggtcagtggg gtgatcagcg cccaggccag
     6901 cagcannnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnngccca gtctggcaaa
     7021 aggcctgtgc cgggataggc ttgatgcaag tcaatgccgt ggcgggcggc ggcgggggag
     7081 gatggctgca ttgatcctgc tgcgagcttt tcttatgctg cgcctgacgc cttatcttgc
     7141 ttcgctcttg caccccacgc cgctggcgcc ggcgcgccgg ccggccgggc cggtggtgat
     7201 ctggaatctg atccgccgct gcaacctcac ctgccagcac tgttattcca tctctgccga
     7261 ccgggatttc cccggtgaat taagcacctc agaagtgttt gccgtgatgg acgatttgcg
     7321 cgggtttggc gtgccggtgc tgattttgtc gggcggcgag cccttgttgc gcgcggatat
     7381 ttttgatatt gccgcccgcg ccaagacgct ggggttttat gtcgggctgt ccagcaatgg
     7441 caccttgatc gacgccggga tggcggcgcg gctggcgggg ctggggctgg attatcttgg
     7501 cgtcagcctg gatggcatgg ccgccaccca cgaccgtttt cggcggctgg agggcggctt
     7561 tgcccgagcg gcggcgggca ttcgcctggc gcgcgacgcc ggcatgaagg tgggcgtgcg
     7621 ctacaccctc accgaagaca acgcggcgga tttgccggcc ttgttgcagt gggtggacga
     7681 cgagggcatc gacaagtttt atttttcgca cctgaactac gccgggcgcg gcaacaagca
     7741 ccgcgacagc gacgcccgcc acgcgcgcac gcgctgggcg atggatttgc tgattgaggc
     7801 ctgtttgcgc cacctgcgcg ccggccaggc caaggatttt gtcaccggca ataacgacgc
     7861 cgacgcggtg tatctgctgc gctgggcgca aacccactac cccgagcagg ccgaggcgct
     7921 gcgccagcgg ctgctggcct ggggcggcaa cgccagcggg gtgaatgtgg ccaatatcga
     7981 caatctgggc aacgttcacc cggacaccat gtggtgggat attcaattgg gcaatgtgcg
     8041 cgagcggccg ttttcgcaga tttggccgga tgtgtcgcac ccgctgatgg ccgggctgaa
     8101 acaacgcccg cgcccggtgc acggccgttg cggcgcgtgt ccgcatctgg ccatctgcaa
     8161 tggcaacacc cgcgtgcgcg cctgggcgct gactggcgac ttctgggcgg ccgacccggc
     8221 ctgctacctg agcgacgccg agctgggcct gccgccaggc aagtatgccc aagccgagct
     8281 caccgcctgg cagcgacaag gggcgacggc atgaggcgcg cctggctgtg gatgggcctg
     8341 gcgctggccg ccagtggcca cgccgccgcg ccggcggcgc tgtatcagca acactgccaa
     8401 agctgccacg gcgagcagcg cctgggcggc atggcgccgg ccttgttgcc gcaaagcctg
     8461 gagcgcagca agccggcaga gttgcaggcc accgtgcgcg acggccgccc ggccacgcaa
     8521 atgcaaggct ttgccgaccg gctgagcgcc gacgagatcc aggccttggt cggctggctg
     8581 cgcacgccag cggccacgcc gccgcgctgg agcgaggccg agatggccgc cagccaccgg
     8641 cagtttgtcc cgctggcgca gctgccagac gcgccaggcc atggcgccga cccggccaat
     8701 ctgtttgtgg tggtggaggc gggcgaccat catgtgtcgg tgctggatgg cgagcgcttt
     8761 accgtgctgg cgcgctttgc cagccattac gcgctgcacg gcgggccaaa gttcagcccg
     8821 gatgggcgct ttgtttactt tgccagccgt gacggctggg tcagccagta tgatttatac
     8881 agcctgcaaa aagtggcgga aatccgcgtc gggctgaaca cccgcaatgt ggcggcctcg
     8941 gccgacgggc gctggctgct ggccggcaac accctgccag aaaacctggt gctgctggac
     9001 gcgcgcaccc tccagccggt gaaaaccctg ccggtgcgca cccagcaagg ccagctgtcg
     9061 cgggtgtcgg cggtgtacga cgccgcgccg cgccagagct ttatcgtcgc actgaaagac
     9121 gcgcccgagc tgtgggaaat ctcctacaac ccggcggccg agccgattta cgatggctat
     9181 gtgcacgact acgccatggg cgagggcctg gccaagccgg gttttctcaa cccccggcgc
     9241 acgccgctgg acgccgtgct ggatgatttt ttctttgacc cggactatcg ccacgtgatc
     9301 ggcgcctcgc gcgacggccg cgcccaggtg gtgaacctgg acatccgccg ccaggtggcg
     9361 gcgctgcaac tggacggcat gccgcatctg ggctcgggca ttaccttcaa gcgcgacggc
     9421 cgcaccctgc tggccacccc caatctgaaa accggccagg tcagcgtgat cgacaccggc
     9481 aactggcagg aagtcacccg catcgccacg ccggggccgg ggttttttct gcgcagccac
     9541 gaacgcacgc cctacgcctg ggtggatgcg atgatgagcg cgcagcgcga cacgctgacc
     9601 ctgatcgaca aacgcactct gcaagtggcg cacaccctgc gcccgcgccc cggcaaaacc
     9661 accgcccatg tggaattcac ccgcgacggc cgctatgcgc tggtcagcgt gatggaaacc
     9721 cccggcgagc tgctggtgat cgacgccgcc accctgcgcg aagtggcggc gctgccgatg
     9781 atgaagccga ttggcaaata caatgtgcac aacaagatca cccgctcgga gggcaccagc
     9841 cactgacaca ggccacgcac gccggcgccc cagctagccc atctgaccga gtgacgcctg
     9901 gccagtcaca cgctaccatg gcgcattgcg cccaacaggc gcttgtttca ggattgtcgc
     9961 catgagccag atcgacgtgc gggcgctatt gcgccatcaa ccgatgtttc accaactcag
    10021 cgacgcgcaa atcgacgcgc tggcgcccgg cgtgcgcgag gtgcgcgggc aaaaaagcca
    10081 ggtggtgttt catcgcggcg atttgtgcga agggctgtat ctggtggtgt acgggcgggt
    10141 gaaactgtcc atcagctcgg cgcagggggt agagaaagtc atcgagatca tccagcccgg
    10201 ccagagcttt gccgaggcgg tgatgttttt gggcacgccc tgcccggtga cggcgcagtt
    10261 tctggaagac ggcctgctct tgcaggtgga agccagcgcc atcgaccgcg ccatggccga
    10321 cgaccccgcc tttgcccgcc gcctgctggc cgggctgtcg atgcgcctgc acagcctgat
    10381 ccgcgacgtt gagcgctatt cgatcgaaag ctccacccag cgcgtgctgg gctatctgct
    10441 gcaatacgcc gacccggaac agggcgcgca acaggtggtc accctgccgg tcaacaaaaa
    10501 cctgatcgcc tcacggctga acctcacccc ggaaaccttc tcgcgcgtgc tgcaccagct
    10561 cagcgacgcc ggcctactgg cggtcaacgg ccgcgacatc accctgctgg atctggacaa
    10621 ggtgcgggcg tttgggtgtg gctgaggctc agagcgtgtg caaaatctgc gagactcggt
    10681 gcgttgaaaa gccctcaaaa atgcgcattg accgtgtgtc aattgcgctt tttgggggca
    10741 tttcgccttg gctgacccgt cgttcgctag acatggaaca cgttctcagg attctggcaa
    10801 agacaaatac gatacgttga gtgttttgat ttcgctgta
//
LOCUS       DAOPYX010000065        10760 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_7048_length_10760_cov_8.032975, whole genome shotgun sequence.
ACCESSION   DAOPYX010000065 DAOPYX010000000
VERSION     DAOPYX010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 10760)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10760)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10760
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7048_length_10760_cov_8.032975"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1253
                     /locus_tag="PKN55_04955"
     CDS             <1..1253
                     /locus_tag="PKN55_04955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012697766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HNX70625.1"
                     /translation="VLVLVLLPPLAGVLGGSSAAGGGAVEGSLWTALGTLAKVAAFIA
                     LMLVVGRRVLPKVLWWVAGAGSRELFTLCVIAAALGVAYGSAKLFDVSFALGAFFSGM
                     MLRESEYSHRAADESLPLRDAFSVLFFVSVGMLFDPAVLLTDPVKVLAVTLVIMVGKT
                     LAAIALVLLMRLPLNTALTVGVSLAQIGEFSFILAGLGVGLNLLPREGQNLILAGAML
                     SIACNSLLFAAITPLEQRLRARYRWARELALRPDPLSELPGTVHSESLSGQVVLVGHG
                     RVGSRVADALLARQVPVVVVERRREAVEALRAQHRPAVVGDGAEPSVLIQAHIARASL
                     LVVATPDLFDARKMVEIARALNPGIAVLARGDDSAEAELWRQEQLGAVVASDAAMARE
                     LVEQIVALRGEPAVHGGHGTASQT"
     gene            1609..2853
                     /locus_tag="PKN55_04960"
     CDS             1609..2853
                     /locus_tag="PKN55_04960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit"
                     /protein_id="HNX70626.1"
                     /translation="MTSFETLIGLRYLRAKRRNSFISFISLISIVGIALGVAALIIVL
                     SVMNGFQKEIRGRILGVASHLEITGMNNRLDNWRGAMAVARQDARVTASAPFVSAQGL
                     LSVGPNVRGAMVRGVLPEAENTVVDVGHKMLVGQLSDLQPGGFGIVLGVELARQLGVG
                     RGDKVTLITPQGQITPAGMMPRLKQFTVVGVFKVDMFEYDASLAMIQLKDAQTLFRLD
                     DAVSGVRLKLNDLMLAPAVKHALAPKLTYDMYLTDWTDQHASYFRAVQIEKRMMFIIL
                     TLIVAVAAFNLVSTLIMTVTDKQADIAILRTLGAAPGSIMRIFVIQGALSGLIGTLCG
                     VGGGVLVALNLDVLVPAIERLLGSKIFSSEVYLIDKLPSDVLPGDVLTITVISLLLAL
                     LATLYPSWRASRTQPAEALRYE"
     gene            2846..3544
                     /gene="lolD"
                     /locus_tag="PKN55_04965"
     CDS             2846..3544
                     /gene="lolD"
                     /locus_tag="PKN55_04965"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003785225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HNX70627.1"
                     /translation="MSDQVPVLAASHVSRIYQEGPLKVSVLADVNLHVAAGERIAIVG
                     ASGSGKSTLLHLLGGLDAPSSGEIRVQGQLLSNLGEAARGALRNRALGFVYQFHHLLP
                     EFTALENVMMPLLIRRLPGKEASARAAAMLDKVGLAARLQHKPGELSGGERQRAALAR
                     ALVTEPACVLADEPTGNLDHATARRVFELMLELNASLNTSLVIVTHDREMARQTGRVL
                     TLEDGQLVDTKETA"
     assembly_gap    3675..3774
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3775..4556
                     /locus_tag="PKN55_04970"
     CDS             <3775..4556
                     /locus_tag="PKN55_04970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009120060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HNX70628.1"
                     /translation="FWLIAIILASMWVSRIIEKRVLGFNHLDMNLRIVLVNLSRTLLV
                     VLGVLIALPVVGIDLTVLSVFGGALGVGLGFGLQKIASNYVSGFIILLERSIRIGDRV
                     SIENRVGYISQITARYTVLKGADGSEALVPNDTLIANTVVNQSYSDKAIWLSIPVQVA
                     YGTDLDLALRVLREAADAVSRVQKDPAPAAFVAAFAENGINLELGVWCGDPENGLLGL
                     RSAVNLEIWRRFAQAGIGMPFPQREVRLLNPSQPTQGSEPT"
     gene            4553..5167
                     /locus_tag="PKN55_04975"
     CDS             4553..5167
                     /locus_tag="PKN55_04975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNX70629.1"
                     /translation="MNPRSVAIVDDDEAVRDALSWLFSSKGLPTLSFASGEALLAAFD
                     VERIGCLVLDVRMAPINGLALFEKLLASQPYCPPAIFLTGHADVPLAVSALKMGAVDF
                     LEKPFDDTVLIERVEAALERDVSARAALASRHQLEAQLRQLTEREREVMALILDGKLN
                     KQIAEALDISIKTVEVHRARVLEKMAVKSAVELAGRLSSLRQEP"
     gene            5171..5629
                     /gene="nudB"
                     /locus_tag="PKN55_04980"
     CDS             5171..5629
                     /gene="nudB"
                     /locus_tag="PKN55_04980"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012697302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008828 - dATP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0019177 - dihydroneopterin triphosphate
                     pyrophosphohydrolase activity [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HNX70630.1"
                     /translation="MGNKQPISVLVVVHTADLQVLLLERADYPGSWQSVTGSREGEET
                     PLQTARRELHEETGLDASHYVLSDWGVQNRYEIYPKWRHRYPDGVTHNTEHVLGLMLP
                     APLAVTLAPREHLAWRWLPWQAAAEQVFSPSNADAIRLLPARLARAAQPA"
     gene            5629..6384
                     /locus_tag="PKN55_04985"
     CDS             5629..6384
                     /locus_tag="PKN55_04985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009118085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HNX70631.1"
                     /translation="MTAHALHIATYNMHKGLSPLNTRLRLPDIARSLKTLAADVVFLQ
                     EVQGRHSARAQRFADWPAEAQHQYLARQLHARATYGLNCAHEHGHHGNAILSRLPVEH
                     WCNRDISVNRFESRGVLHCDFRPPGWHSGLTALCVHLNLLARDRKKQFAALRDYIAHA
                     VPAEHALIIAGDFNDWRAQANDLLAGELGLSEVFHTLHGAHARSYPARLPMLTLDRIY
                     TRGLRVTQAQVHHGAPWAQLSDHLPLSATLLPE"
     gene            6389..7582
                     /gene="clsB"
                     /locus_tag="PKN55_04990"
     CDS             6389..7582
                     /gene="clsB"
                     /locus_tag="PKN55_04990"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF008427.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HNX70632.1"
                     /translation="MGRLWRHNAVTLLCDGAEFFPALLASLNAARRQIQLETYIFRID
                     ATGERIARALMAAARRGVEVNVLLDGFGCADFPDHWLNALRAAGVNVRFFRPEVLRWW
                     PRRSRLRRLHRKLAMIDAHTAFVGGINLHDDRDNAGPEDAPRYDYAVRVCGPVVADIQ
                     HTMRQLWLSVAFGPLVARGPQALTRLLSKSQELHADTRPAGVQSARLLVRDNGRHRLS
                     IEQSYLRQIRRAQHEILIANAYFLPGLRLRHALCDAARRGVKVKLLLQGRVDHAILHY
                     ATRMLYRDFLNSGIEIHEYQAGYMHAKVAVIDRRWAMVGSSNIDPFSLLLAREANLQI
                     QHADFAQGLRRHMLNKIQRHARQVAPQELTQASLPKRALVWLCYGLVRAGIQVSGYGG
                     GYYQE"
     assembly_gap    7588..7687
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(7870..9834)
                     /gene="acs"
                     /locus_tag="PKN55_04995"
     CDS             complement(7870..9834)
                     /gene="acs"
                     /locus_tag="PKN55_04995"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011136829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HNX70633.1"
                     /translation="MSTIESVLKETRSFAPSDAFRQKATISGMEAYNALCEQANQDYA
                     GYWGGLARDLLTWHKPFTQVFDDSNAPFFKWFADGELNVSYNCIDRHLAQSADKVALI
                     FEADGGEVENITYAELHRRVCQFANGLKSLGVVKGDRVVIYMPMVAEAIVAMQACARI
                     GAIHSVVFGGFSAGAVKDRIEDAGAKLVITANEGLRGGKNVPLKATVDEALAMDGTAS
                     VQKVVVYQRTPTQVAWTEGRDVCWTELTKDQPVTCEPEWMNAEDPLFILYTSGSTGKP
                     KGIQHSTAGYLLGAMNSFRTVFDFKQDDVYWCTADVGWITGHSYICYGPLANGATQVV
                     FEGVPTYPNASRFWRMIQDHKVSIFYTAPTAIRSLIKLGSDLPNQFDLSSLRLLGTVG
                     EPINPEAWMWYYEVVGGSRCPIVDTWWQTETGSAMIAPLPGAVPTKPGSCTLPLPGII
                     ADIVDESGAQVEPGRGGFLVIKKPFPSLVRTIWGDPDRFKKTYFPEEFNGQYYLAGDS
                     ANRDENGYFWIMGRIDDVLNVSGHRLGTMEIESALVANPLVAEAAVVGKPHDVKGEAV
                     VAFVVLKGARPEGEEAKKIAAELKAWVAHEIGKIAQPDDIRFGENLPKTRSGKIMRRL
                     LRSIAKGEEITQDVSTLENPHILGQLKDAV"
     gene            complement(9911..>10760)
                     /gene="actP"
                     /locus_tag="PKN55_05000"
     CDS             complement(9911..>10760)
                     /gene="actP"
                     /locus_tag="PKN55_05000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008954292.1"
                     /note="member of the sodium:solute symporter family;
                     cotranscribed with the acs gene which encodes acetyl
                     coenzyme A synthase; mutations affect acetate uptake;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cation acetate symporter"
                     /protein_id="HNX70634.1"
                     /translation="LMFGTAGLPHILMRFFTVSNAKEARKSVFFATGFIGYFYILTFI
                     IGFGAIFLVGTNPEFKDAAGKLIGGNNMAAVHLAKAVGGNLFLGFISAVAFATILAVV
                     AGLALSGASAVSHDLYASVIKKGKADEAAEMRVSKITTLVLGVIAIVLGIIFEKQNIA
                     FMVGLAFSIAASANFPVLFLSMFWKGLTTRGAVVGGFVGLFSAVLLIVLGPAVWVDVM
                     GNAKGSAPFAYKNPALFSMTLAFVTTWLVSVMDNSKQAQEERKLFDAQFVRAMTGIGA
                     AGASKH"
BASE COUNT         1769 a         3255 c         3546 g         1990 t
ORIGIN      
        1 tggtgctggt gctggtgttg ctgccgccct tggccggcgt gctgggcggc agtagtgcgg
       61 cgggcggcgg cgcggtggaa ggttcgctgt ggacggcgct gggcaccctg gccaaggtgg
      121 cggcgtttat tgcgctgatg ctggtggtgg gccggcgggt gttgcccaag gtgctgtggt
      181 gggtggccgg ggcgggctcg cgcgagctgt tcaccctgtg tgtgattgcc gccgcgctgg
      241 gcgtggcgta tggctcggcc aagctgtttg atgtgtcgtt tgcgctgggg gcgtttttct
      301 cggggatgat gctgcgcgag tcggaataca gtcaccgcgc ggcggatgaa tcgctgccgc
      361 tgcgcgatgc tttttcggtg ctgttttttg tgtcggtggg gatgttgttc gatccggcgg
      421 tgctgctgac cgatccggtc aaggtgctgg cggtcacgct ggtgatcatg gtcggcaaaa
      481 ccctggcggc gattgccttg gtgttgctga tgcgcctgcc gctgaacacc gcgctgactg
      541 ttggtgtgag cctggcgcag attggcgagt tttcgtttat cttggccggg ctgggcgtcg
      601 ggctgaactt gctgccgcgc gaggggcaaa acctgattct ggccggcgcc atgttgtcga
      661 ttgcctgcaa cagcctgctg tttgccgcca tcaccccgct ggagcagcgc ttgcgcgcgc
      721 gctaccgctg ggcgcgcgag ctggccttgc ggcctgatcc cctgtccgag ctgcccggca
      781 cggtgcatag cgagtcgctg agcggccagg tggtgttggt cggccatggc cgggtaggct
      841 cgcgcgttgc cgatgcgctg ctggcgcggc aggtgccggt ggtggtggtc gagcgccgac
      901 gcgaggcggt ggaggcgctg cgcgcccagc atcgcccggc ggtggtgggc gacggcgccg
      961 agccctcggt gctgattcag gcgcatatcg cccgcgccag cctgttggtg gtggccaccc
     1021 cggatttgtt tgacgcgcgc aagatggtgg agatcgcccg tgcgctcaac cccggcattg
     1081 ccgtgctggc gcgcggcgac gacagcgccg aagccgagct gtggcggcag gagcagctgg
     1141 gcgcggtggt ggccagcgat gcggcgatgg cgcgcgagct tgtggaacag attgtggcct
     1201 tgcgcggcga acccgccgtg catggtggtc atggcacggc gagccagact tgaggcacgt
     1261 agccgctcgt ctgtcgcagg gctgccgttt tttggcgcag ctcacaaaac ccgacgtggc
     1321 ggtctgggtc aagcgtgtta agcgttgtgt gggcgtgtcg ggatggtcgc ttgggtgcac
     1381 accacacccc tgctgcccag ccgttccaga gtaagcccgc ccgattgtgc cggtgtgctc
     1441 agtacgccgg ggagggaacc ccagccgcgc ggtggcagtc tgctacaggg acgccgccag
     1501 ggtattacac tcgccgaccc tcatcgacat gggcataata cgccgtgtgg tgttcggcct
     1561 tggccgcgcg cattccccgc aacttcccgc ccgcgtggcg atggtgtcat gacttcattc
     1621 gaaaccctga tcgggctgcg ttatctgcgc gccaagcggc gcaatagctt tatttctttt
     1681 atttccctga tttccattgt cggcatcgcc ctgggtgtgg cggcgctgat tattgtgctg
     1741 tcggtgatga acggttttca gaaagaaatc cgtggccgca ttctgggcgt ggcctcgcat
     1801 ctggaaatca ccggcatgaa caaccggctg gacaactggc gcggcgccat ggcggtggcg
     1861 cgtcaggatg cgcgggtgac ggccagcgcg ccgtttgtgt cggcgcaggg cctgctgtcg
     1921 gtggggccga atgtgcgcgg ggcgatggtg cgcggcgtgc tgcccgaagc agaaaacacc
     1981 gtggtggatg tcggccacaa gatgctggtg ggccagctca gcgatttgca gccgggcggc
     2041 tttggcattg tgctgggcgt cgagctggcg cggcagttgg gcgtgggccg tggcgacaag
     2101 gtgacgctga tcaccccgca agggcagatc accccggccg ggatgatgcc ccggctcaag
     2161 cagttcaccg tggtgggggt gttcaaggtg gatatgtttg aatacgacgc ctcgctggcg
     2221 atgatccagc tgaaagacgc gcagacttta ttccgcctgg acgacgccgt gtccggggtg
     2281 cggctcaagc tgaatgattt gatgctggcg ccagcagtca agcatgcctt ggcgcccaag
     2341 ctcacttacg atatgtatct gaccgactgg accgaccagc acgccagtta ttttcgggcg
     2401 gtgcagatcg aaaaacgcat gatgttcatc attctgacgc tgattgtcgc cgtggcggcg
     2461 tttaatctgg tgtccacgct gatcatgacc gtgaccgaca aacaggccga catcgccatc
     2521 ttgcgcacgc tgggcgcggc gccaggctcg atcatgcgca tttttgtcat tcagggcgcg
     2581 ttgtccggcc tgattggcac gctgtgcggc gtgggcggcg gcgtgctggt ggcgctgaat
     2641 ctggatgtgc tggtgccggc catcgaacgg ctgctgggca gcaagatttt ttcttccgag
     2701 gtgtacttga tcgacaaact gccgtctgac gtgttgccgg gcgatgtgct gaccatcacg
     2761 gtgatttccc tgctgctggc cttgctggcc acgctttacc ccagctggcg cgcctcgcgc
     2821 acgcaaccgg cggaggcgct gcgctatgag tgatcaggtt ccggtgctgg cggccagcca
     2881 tgtcagccgc atttatcagg aaggcccgct gaaggtcagc gtgctggccg atgtcaattt
     2941 gcacgtggcg gccggcgagc gcatcgccat tgtcggcgcc tctggctcgg gcaagagcac
     3001 gctcttgcac ctgttgggcg ggctggatgc gcccagcagc ggcgaaatcc gcgtgcaggg
     3061 ccagttgctg tccaatctgg gcgaggcggc gcgcggcgcg ctgcgcaacc gcgcgctggg
     3121 ctttgtctat cagtttcatc atctattgcc agaattcacc gcgctggaaa acgtgatgat
     3181 gccgctgctg attcgccgcc tgcccggcaa agaggccagc gcgcgcgcgg cggccatgct
     3241 ggacaaagtg ggcctggcgg cgcgcttgca gcataagccg ggcgaattgt ctggcggcga
     3301 gcgccagcgg gcggcgctgg cgcgcgcctt ggtcaccgag ccggcctgcg tgctggccga
     3361 cgagcccacc ggcaatctgg accacgccac cgcccggcgg gtgttcgaat tgatgctgga
     3421 gctgaacgcc agcctgaaca ccagcctggt gattgtcacc cacgaccgcg agatggcgcg
     3481 ccagaccggc cgggtgctga ccctggaaga cggtcaattg gtagatacca aggagacggc
     3541 atgagcgccc tgaaccctcg taatttgtct tttgaagccc tgctggccac cctggccgaa
     3601 ccgcaggcac aaatcgacct ggcgcttggc ctggcctggg cggtgctggc ctggtggctg
     3661 gcgcgcgtgc tgacnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnttttct
     3781 ggctgatcgc cattatcctg gcgtcgatgt gggtgtcgcg catcatcgaa aagcgcgtgc
     3841 tgggctttaa tcacctggat atgaacctgc gcattgtgct ggtcaatctg tcgcgcaccc
     3901 tgctggtggt gctgggcgtg ctgattgcct tgccggtggt gggcattgat ctgaccgtgt
     3961 tgtcggtctt tggcggcgcg ctgggcgtgg gcctgggctt tggcctgcaa aaaatcgcca
     4021 gcaattatgt gtccggtttt atcatcctgc tggagcgctc gatccgcatt ggcgaccgcg
     4081 tcagcatcga aaaccgggtg ggctatatca gccagatcac cgcccgctac accgtgctca
     4141 agggcgccga cggctcggag gcgctggtgc caaacgacac gctgattgcc aacaccgtgg
     4201 tgaatcaatc gtattcggac aaggccatct ggctgtcgat tccggtgcag gtggcctacg
     4261 gcacggatct ggacctggcc ttgcgcgtgc tgcgcgaggc ggccgatgcg gtgtcgcgcg
     4321 tgcaaaaaga cccggcgccg gcggcgtttg tggcggcgtt tgccgagaat ggcattaatc
     4381 tggagctggg cgtgtggtgc ggcgacccgg aaaacggcct gctgggcctg cgctcggcgg
     4441 tgaacctgga aatctggcgc cgctttgccc aggccggcat cggcatgccg tttccgcagc
     4501 gggaagtgcg cctgctcaac ccatcccaac cgactcaggg gagcgagccc acatgaatcc
     4561 acgcagtgtg gcgattgtcg atgacgacga agccgtgcgc gatgcgctgt cctggttgtt
     4621 cagctccaaa ggcctgccca cgctgagctt tgcctcgggc gaggcgctgc tggcggcgtt
     4681 tgacgtcgag cgcatcggct gcctggtgct ggacgtgcgc atggcgccga tcaacggcct
     4741 ggccttgttt gaaaaactct tggccagcca gccctattgc ccgccagcga tttttttgac
     4801 cggccacgcc gatgtgccgc tggcggtgtc ggcgctgaaa atgggcgcgg tggatttttt
     4861 ggaaaaaccc ttcgacgaca ccgtgttgat cgagcgggtg gaagccgcgc tggagcgcga
     4921 tgtcagcgcc cgcgccgccc tggccagccg ccaccagctg gaagcccagc tgcgccagct
     4981 gaccgagcgc gagcgcgagg tgatggcgct gattctggac ggcaagctga acaagcaaat
     5041 cgccgaggcg ctggacatca gcatcaagac cgtcgaagtc caccgcgcgc gcgtgctgga
     5101 aaaaatggcg gtcaaatccg ccgtcgagct ggccggccgg ctgtccagcc tgcgtcagga
     5161 gccgtgatcc atgggcaata agcaaccgat ttccgtgctg gtggtggtgc acaccgccga
     5221 tttgcaggtg ctgctgctgg agcgggccga ttaccccggc tcctggcaat cggtgaccgg
     5281 cagccgcgag ggcgaagaaa ccccgctgca aaccgcccgg cgtgagctgc acgaagaaac
     5341 cggcctggac gccagccact acgtgctgag cgactggggc gtgcaaaacc gctatgaaat
     5401 ctaccccaaa tggcgccacc gctacccgga cggcgtcacc cacaacaccg aacacgtcct
     5461 gggcctgatg ctgccggcgc cgctggcggt gacgctggcg ccgcgcgagc atctggcctg
     5521 gcgctggctg ccctggcaag ccgccgccga gcaggtgttt tctccatcca acgccgacgc
     5581 catccgcctg ctgcccgcgc ggctggcccg cgccgcccag cccgcctgat gaccgcccac
     5641 gccctgcaca tcgccaccta caatatgcac aagggcttaa gcccgctgaa cacccggctg
     5701 cgcctgcccg acatcgcccg cagcctgaaa accctggccg ccgacgtggt gtttttgcaa
     5761 gaggtgcaag gccggcacag cgcgcgcgcc cagcgctttg ccgactggcc ggccgaggcg
     5821 cagcaccaat acctggcccg ccagctgcac gcccgcgcca cctatgggct gaactgcgcg
     5881 cacgaacacg gccaccacgg caacgccatt ttgtcgcggc tgccggttga gcactggtgc
     5941 aaccgggata tttcggtcaa ccgctttgaa tcgcgcggcg tgctgcattg cgattttcgc
     6001 ccgcccggct ggcacagcgg gctgaccgcg ctgtgcgtgc accttaacct gctggcgcgc
     6061 gaccgcaaaa agcagtttgc cgcgctgcgc gactacatcg cccacgccgt gccggccgag
     6121 catgcgctga tcatcgccgg ggatttcaac gactggcgcg cccaggccaa tgatctgctg
     6181 gccggcgagc tgggcctgag cgaggtgttt cacaccctgc acggcgcgca tgcacgcagc
     6241 tacccggcgc ggctgcccat gctgacgctg gaccgcattt acacccgtgg cctgcgcgtc
     6301 acccaggcgc aggtgcatca cggcgcgccg tgggcgcagc tgtctgatca cctgccgctg
     6361 tcggccacct tgttgccgga gtaaacgcat gggccggctg tggcgccaca atgcggtgac
     6421 tttgctgtgc gacggcgccg agttttttcc ggcgctgctg gccagcctga acgccgcccg
     6481 gcggcaaatc cagctggaga cctatatctt tcgcatcgac gccaccggcg agcgcattgc
     6541 ccgcgcgctg atggccgccg cccgtcgcgg ggtggaagtc aatgtgctgc tggatggctt
     6601 tggctgtgcg gattttcctg atcactggct gaacgccctg cgcgcggccg gggtgaatgt
     6661 gcggtttttt cgccccgagg tgctgcgctg gtggccgcgc cgcagccgcc tgcgccgcct
     6721 gcaccgcaag ctggcgatga tcgacgccca caccgcgttt gtcggtggca tcaatctgca
     6781 cgacgaccgc gacaacgccg gcccggaaga cgcgccgcgc tacgactacg ccgtgcgcgt
     6841 gtgcggcccg gtggtggccg atattcagca caccatgcgc cagctgtggc tgagcgtggc
     6901 ctttggccca ctggtggcgc gcggcccgca ggcgctcacc cgcttgttga gcaagagcca
     6961 ggaactacac gccgacaccc gcccggccgg cgtgcaatcc gcccggctgt tggtgcgcga
     7021 caacggccgc caccggctgt ccatcgaaca aagctatctg cggcaaatcc gccgcgccca
     7081 gcacgaaatc ctgatcgcca atgcctactt tttgcccggc ctgcgcctgc gccacgcgct
     7141 atgcgacgcc gcccggcgcg gggtgaaggt caagctgctg ctgcaaggcc gggtggacca
     7201 cgccatcctg cactacgcca cccgcatgct gtaccgggat tttctcaata gcgggattga
     7261 aatccacgaa taccaggcgg gctatatgca cgccaaagtg gcggtgatcg accggcgctg
     7321 ggccatggtc ggctcgtcca atatcgaccc gttcagcctg ctgctggcgc gcgaggccaa
     7381 tctgcaaatc cagcacgccg actttgccca aggcctgcgc cggcacatgc tgaacaaaat
     7441 ccagcgccac gcccgccaag tggccccaca ggagctgaca caagcctccc tgcccaaacg
     7501 cgcgctggtg tggctgtgct acggcctggt gcgcgccggg attcaggtgt cggggtatgg
     7561 tggcgggtat tatcaggagt gagaagcnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7681 nnnnnnnaaa gtggcaaagt ggtgcaacaa cgccaagagg gggttgttcg ccacgctgac
     7741 tatgcagatc agccacacaa acaaaaaaaa ccgcacctgt ccggaaaacc cggacaaggc
     7801 gcggcaatcg cagggaaacg gacttccagc tcgccgggca ggcgcgagcc ggaaggtaaa
     7861 acacatcaat tacacagcgt ctttcagctg gcccaggatg tgcgggtttt ccagggtgga
     7921 cacgtcctgg gtgatctctt cgcccttggc gatggagcgc agcaggcggc gcatgatctt
     7981 gccggaacgg gtcttgggca ggttttcgcc aaagcggatg tcgtccggct gggcgatctt
     8041 gccgatttcg tgggccaccc aggctttcag ttcagcggca atcttcttgg cttcttcgcc
     8101 ttccggacga gcgcccttga gcaccacgaa ggccaccacg gcttcgccct tcacatcgtg
     8161 cggcttgccc accacggcgg cttcggccac cagcgggttg gccaccaggg cggattcgat
     8221 ttccatggtg cccagacggt ggccggacac gttcagcacg tcgtcgatgc ggcccatgat
     8281 ccagaagtag ccgttttcgt cgcggttggc cgagtcgcca gccaggtagt attggccgtt
     8341 gaactcttcc gggaagtagg ttttcttgaa gcggtccggg tcaccccaga tggtgcgcac
     8401 cagcgacggg aacggcttct tgatcaccag gaaaccgcca cggcccggct caacctgggc
     8461 gccggattcg tcaacgatgt cagcgatgat gcccggcagc ggcagggtgc aagaacccgg
     8521 cttggtcggc acagcgcccg gcagcggggc gatcatcgcc gagccggttt cggtttgcca
     8581 ccaggtgtcc acgatcgggc aacggctgcc gcccaccact tcgtagtacc acatccaggc
     8641 ttccggattg atcggctcac ccacggtgcc cagcaggcgc aggctggaca ggtcgaactg
     8701 gttcggcagg tcggaaccca gcttgatcag cgagcggatg gcggtcgggg cggtgtagaa
     8761 gatcgagacc ttgtggtctt gaatcatgcg ccagaagcgc gaggcgttcg ggtaggtcgg
     8821 cacgccttca aacaccacct gggtggcgcc attggccagc gggccgtagc agatgtagga
     8881 gtggccggtg atccaaccca catcggccgt gcaccagtaa acgtcgtctt gcttgaagtc
     8941 gaacacggtg cggaaggagt tcatcgcgcc cagcagatag ccggcggtgc tgtgctgaat
     9001 acccttgggc ttgccggtgg agccggaggt gtacaggatg aacagcggat cttcggcgtt
     9061 catccattcc ggttcgcagg tcaccggctg gtctttggtc agctcggtcc agcacacgtc
     9121 gcggccttcg gtccaggcga cctgggtcgg ggtgcgttgg tacaccacca ctttttgtac
     9181 cgaggccgtg ccgtccatgg ccagcgcttc gtccacggtg gccttcagcg gcacattctt
     9241 gccgccgcgc aggccttcat tggcggtgat caccagcttg gcgccggcat cttcgatacg
     9301 gtctttcacg gcgccggcag agaagccgcc gaacaccacg gaatggatgg cgccgatgcg
     9361 ggcgcaagcc tgcatggcga caatggcttc ggccaccatc ggcatgtaga tgaccacacg
     9421 gtcacccttg accacgccca ggctcttcag gccgttggcg aactgacaca cacggcggtg
     9481 cagttcggcg taagtaatat tttcgacttc gccaccgtcg gcttcaaaaa tcagcgcgac
     9541 tttgtcggcg ctttgcgcca gatgacggtc gatgcaattg taggagacgt tgagctcacc
     9601 atcggcaaac catttgaaga acggcgcatt gctgtcgtcg aacacctggg tgaacggctt
     9661 gtgccaggtc agcagatcgc gcgccaggcc gccccagtag ccggcgtaat cctgattggc
     9721 ctgctcgcac agggcgttgt aggcttccat gccggagatg gtggcctttt ggcggaaggc
     9781 atcgctcggc gcaaagctgc gagtttcttt caggacggat tcgatggtgg acattcagta
     9841 gctcctttgt agtgcagggg ggaggccgac cgcgcccgaa agcgcggcgg cttgtttctt
     9901 gagtttctga ttagtgcttg gaggcgccag ctgcaccgat gccggtcatg gcgcggacga
     9961 actgggcgtc aaacagcttg cgttcttctt gagcctgctt ggagttgtcc atcacagaca
    10021 ccagccaggt ggtcacaaag gccagggtca tggagaacag cgccgggttc ttgtaagcga
    10081 acggagccga gcccttggcg ttgcccatca cgtcaaccca cactgccggg ccgaggacga
    10141 tcagcagcac ggcggagaac aggccgacaa agccgcccac cacagcgcca cgggtggtca
    10201 gacccttcca gaacatggac aggaacagca ccgggaagtt agccgaagcg gcgatggaga
    10261 aggccaggcc caccatgaag gcgatgttct gcttttcgaa gatgatgccc aggacgatgg
    10321 cgatcacgcc cagcaccaga gtggtgatct tggacacgcg catttcagca gcttcgtcag
    10381 ccttgccctt cttgatcacc gaggcgtaca ggtcatgcga cacggcagag gcgccagaca
    10441 gagccagacc tgccaccacg gccaggatgg tggcgaaagc cacagccgag atgaagccca
    10501 ggaacaggtt gccgcccacg gccttggcca ggtgcacagc agccatgttg ttgccgccga
    10561 tcagcttgcc ggctgcgtcc ttgaactcgg ggttggtgcc caccaggaag atggcgccaa
    10621 aaccgatgat gaaggtcagg atgtagaagt aaccgatgaa gccagtggcg aagaacaccg
    10681 atttgcgggc ttctttggcg ttggacacgg taaagaagcg catcaggatg tgcggcaggc
    10741 cagcggtgcc gaacatcagg
//
LOCUS       DAOPYX010000066        10724 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_7092_length_10724_cov_10.865873, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000066 DAOPYX010000000
VERSION     DAOPYX010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 10724)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10724)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10724
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7092_length_10724_cov_10.865873"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..416)
                     /locus_tag="PKN55_05005"
     CDS             complement(<1..416)
                     /locus_tag="PKN55_05005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011134351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron transporter"
                     /protein_id="HNX70635.1"
                     /translation="MRHRASQLALAALLASVCVSASAKEYPIGKPAIKNGMEIGAVYL
                     QPTKMEPEGMMRKAEDSDIHLEADIHAVKNNPNGFEEGAWMPYLLIKYEISKVGGKEV
                     IKGDMMPMVANDGPHYGDNIKLQGPGKYQLKLTVLPP"
     gene            667..828
                     /locus_tag="PKN55_05010"
     CDS             667..828
                     /locus_tag="PKN55_05010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX70636.1"
                     /translation="MQQTSTHSGKPLATGHGSTAGSASYAEKSAGADILANRHIGRIY
                     FFYYKALQV"
     gene            986..1735
                     /locus_tag="PKN55_05015"
     CDS             986..1735
                     /locus_tag="PKN55_05015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX70637.1"
                     /translation="MPNTVYASYNGSQKGFTVRDLRLNQTFFLNTGFDISGIAAGPNS
                     DVYLTSANNIYHYKADGTLLQKMTFPDTGIKYIGITATSDRVYATYTGSQVGVTVRDL
                     NLNQLSCFGTAFTASGIAAGDASDLYLTAQNHIYHYNVNGTLISDMAFPVTSINYTDV
                     TVLDDRVFASYNGSQLGFTVRDRNLNQLSYASTGFNINSIAAGKSNTVYLASANHLYI
                     YSAAGSLIYDMNFPDHGVNYTGVSAVFLQQT"
     gene            complement(1813..2181)
                     /locus_tag="PKN55_05020"
     CDS             complement(1813..2181)
                     /locus_tag="PKN55_05020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018150364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNX70638.1"
                     /translation="MLSPDMTILAVADPLVSARFYQSLLGIEPVECQATFAMFVLPSG
                     LKLGLWQREGVAPAVTAEPGSVELAAALADEAALRDCWARWRGLGVEVIQPPTQMDFG
                     LTMTAADPDGHRLRVFVPAM"
     gene            2562..3608
                     /locus_tag="PKN55_05025"
     CDS             2562..3608
                     /locus_tag="PKN55_05025"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014088005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006146 - adenine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HNX70639.1"
                     /translation="MTPATDPALQLALTMPKAELHVHIEGTLEPELAFALAQRNGISL
                     PYADQAALAAAYNFDSLQSFLDLYYACADVLRTADDFRDLMLAYLERAAADHIIHTEI
                     FFDPQTHTARGIPFATVLDGLEDGLQQGRARWGISGRLILCFLRHLSEEDALATLAEA
                     EPHLARIDGFGLDSSEQGNPPSKFQRLFARCRALGKPVVAHAGEEGPPDYIIQALDLL
                     GVKRIDHGVRASEDPALLARLAADGVALTVCPLSNLKLRVFPDMHAHTLGQLLAAGVK
                     VTLNSDDPAYFGGYLNDNIRAVQAAFQFDAATWKQLAKNSFEASFASEAEKAGWVERV
                     EVWFGGSVRPKRHS"
     gene            3804..4319
                     /gene="gpt"
                     /locus_tag="PKN55_05030"
     CDS             3804..4319
                     /gene="gpt"
                     /locus_tag="PKN55_05030"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: protein motif:HMM:NF006613.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HNX70640.1"
                     /translation="MEHNPYRDDVVISWAELHRDTRRLCHELMAKGPFKGIIAIARGG
                     LIPAALVARELEIRLIDTICAASYESSDDDSAQTQRKEVRILKGIEHDGEGYLLIDDL
                     VDTGNTARVIRQLLPKAYFVTLYAKPMGRQVVDLCVKEFSQDKWVYFPWDIDYQFVPP
                     IKKRLKAEQQG"
     assembly_gap    4385..4484
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4513..7137)
                     /gene="pepN"
                     /locus_tag="PKN55_05035"
     CDS             complement(4513..7137)
                     /gene="pepN"
                     /locus_tag="PKN55_05035"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018748686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HNX70641.1"
                     /translation="MSASAPVAKYLKDYRPAPFLIDRVDLEFDILDSHTSVRARMVVK
                     RNPKAEACDTLQLDGSAELVAVRLDGEALAPSAWTLADEVLTLPGVPESFILDIETRL
                     EPAANTSLMGLYASNGNLYTQCEPEGFRKITYYLDRPDVMAKFSTTLIADRQRFPVLL
                     SNGNKVGEGLTENRKRHWVKWVDPYRKPAYLFAVVAGKLSVLRDHYTTRGGRKVTLEI
                     YVAPADMDKTAHAMSSLKKSMQWDEQVFGLEYDLDTYMIVAVGDFNMGAMENKGLNIF
                     NTKFVLANRETATDFDFEGVEAVIAHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQE
                     FSADMQSRAVKRIEDARGLRALQFPEDAGPTAHPVRPESYIEMNNFYTLTVYEKGAEV
                     VRMIHTLVGAAGFRQGMDLYFRRHDGQAVTCEDFVAAMADANHIDLTQFKRWYSQAGT
                     PVLDVSLDWFAEGGAASLTVRQHCPDTPGQTDKLPFHIPLAVGLLAADGSELPVTLAS
                     DDAPGPLTRVLSVTEAEQTFTFVGLSEKPLPSLLRNFSAPVRLNYAYRDEELAFLCAH
                     DSDAFNRWEAGQTLATRELLRLYRGEGEATLSACLIDAFAQLLDHADSDPAFAALALT
                     LPAEVELLELQDHADPARLCQVREQARAELAQALAERWDAAWRRHSGLAFRHEDAGRR
                     ALKNLALSYLALLPGGDAGALASQQYQQADNMTDRMGALAALADSHSHARDTLFADFA
                     QRFADEALVMDKWFTLQAGSRRQDVLRTVTALMEHPAFALTNPNKVRALIGAFGRNLA
                     AFHAADGSGYRFMADQVLAIDAINPQVASRLVSCFNRRSRVEPARAELMRVELARVLA
                     HDGLSRDVYEIVSKNLNA"
     gene            complement(7472..8014)
                     /gene="uraD"
                     /locus_tag="PKN55_05040"
     CDS             complement(7472..8014)
                     /gene="uraD"
                     /locus_tag="PKN55_05040"
                     /EC_number="4.1.1.97"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008954803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019628 - urate catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline
                     decarboxylase"
                     /protein_id="HNX70642.1"
                     /translation="MNHSLATDMPAFLTLAQLSALDNEGFVAAVGAVFEHSPWVMRRA
                     WARRPFADRAALTAALTATLHAASRDEQLALMCAHPELAGKAAIRGELTADSAREQAG
                     AGLNACTPAEFAELQALNLAYRQRFGWPFIIAVTGLSRTDILAAMRQRLARAPEDEFA
                     EALSQIVRIANIRLNALLDE"
     gene            8224..8580
                     /gene="uraH"
                     /locus_tag="PKN55_05045"
     CDS             8224..8580
                     /gene="uraH"
                     /locus_tag="PKN55_05045"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008954802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019628 - urate catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HNX70643.1"
                     /translation="MGRLTTHALDTAHGCPAAGLAYTLYRLDAAGARQLLAHGHTNAD
                     GRADAPLLADAALLAGVYELEFAAGDYFRCRGVTLPEPPFLDVVSLRFGVADPAGHYH
                     VPLLVSPWAYSTYRGS"
     gene            8580..9776
                     /locus_tag="PKN55_05050"
     CDS             8580..9776
                     /locus_tag="PKN55_05050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014085699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate hydroxylase PuuD"
                     /protein_id="HNX70644.1"
                     /translation="MDSGYWLEIASLLLRWLHLIVGIAWIGSSFYFVWLDNSLRPPTD
                     PALREKGVGGELWAVHGGGFYNPQKYLVAPKQLPDELHWFKWESYSTWLSGFALISVL
                     YYAQADSYMIDPAKAALSPGQAVALGLATLAGGWLVYDGLCRLLMRRSILLFAALYFA
                     FVVAVAWGLTHWLSGRAAFIHVGAMLATTMSGNVFFWIIPGQKKTVAAMRRGDAPDPI
                     HGQRAKQRSVHNNYLTLPVLFCMISNHYALTYNHPQAWLVLALILLAGVLIRHFFNLR
                     HHGRLAWHYPLAGVALLLGVFIWLAPKAPTPAAQGGAPAPTLAEVRPVIAARCLACHA
                     SAPTLMAAAPAGLKLDSDEALYAQRQRVVDQAVRLKAMPLGNVTQMTDAERALLARWA
                     AGGFAR"
     gene            9818..>10724
                     /locus_tag="PKN55_05055"
     CDS             9818..>10724
                     /locus_tag="PKN55_05055"
                     /EC_number="3.5.4.3"
                     /inference="COORDINATES: protein motif:HMM:NF006679.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine deaminase"
                     /protein_id="HNX70645.1"
                     /translation="MPTSTTQLIRGHILHFLADPGEHDAADSYQSLPDGALWIVDGRV
                     ADLGPWPELRARRTPDELAGAECFDYGQHLVLPGLVDTHCHYPQSGVIASFGRQLLDW
                     LNDYTFPAEAAFADPCVAEAAAEYFVERLLAHGTTTASVFATVHPQSVDAFMAAAERR
                     QLRMLCGKVMMDRHAPPALCDDVASAERDSLALIERWHRRGRLRYSLTPRFAPTSTPE
                     QLALAGALYRSQPDLHVQSHLAENPDEIAWVGQLFPQRRDYLDVYAHYGLLGPRTIYG
                     HCIHLAPEEIRDMAHTGTAAAFCPTS"
BASE COUNT         1922 a         3492 c         3264 g         1946 t
ORIGIN      
        1 ggcggcagca cggtgagctt gagctgatac ttgcccggcc cttgcagctt gatgttgtcg
       61 ccgtagtgcg ggccatcgtt ggccaccatc ggcatcatgt cgcccttgat cacttccttg
      121 ccgcccacct tgctgatttc gtacttgatc agcagatacg gcatccaggc gccttcttca
      181 aagccgttgg ggttgttttt caccgcgtgg atgtcggctt ccagatggat gtcggaatct
      241 tccgccttgc gcatcatgcc ttccggctcc atcttggtcg gctgcagata caccgcgccg
      301 atttccatgc cgttcttgat ggccggtttg ccgatcgggt attccttggc cgacgcactg
      361 acgcagaccg aggccagcag cgcggccagg gcgagttgac ttgcacgatg acgcatgaca
      421 atgcctttct gtgtgagatg aaaacaccgc gccagccgcc ggcgcaggga ggtttcatct
      481 tactggactt cagtttattt tttcaatggg attggtaaca atttgtattt actttttgga
      541 caggcttggg cggcatggct gactgcacga tgagcagacg ttgacagagg cgatcaggat
      601 ggcaatgcag ccagcattgg gctgcatgtg gcaatgctgc gtgtcgcagc atcaggtgtg
      661 gctggcatgc aacagaccag cacgcattcg ggaaaacctt tggctaccgg gcatggcagc
      721 acggcgggca gtgcgagcta cgctgaaaaa tcagccgggg ccgatattct ggccaaccgg
      781 catatcggta gaatttattt cttttattac aaggcattac aggtttgaat tttccggaaa
      841 cagcctgccg tacactggcc ccgggtgaat aaccgaacgc ggtgcttaca cgatggccaa
      901 gccatcttcc gcccgctctg tcactgtgtg acacctcacc tcacggactg tgatgttcca
      961 ccatttcatg tttctggaga ccgccatgcc caacactgtt tatgcatcgt acaacggctc
     1021 gcaaaaaggc ttcaccgtac gcgacctgcg actgaaccag acgttcttcc tgaataccgg
     1081 ttttgacatc agcgggattg ctgccgggcc gaacagcgat gtgtacctga cctcggccaa
     1141 caatatctac cactacaagg cagatggcac actcctccag aaaatgacct ttccggacac
     1201 cggcatcaaa tacatcggca tcaccgccac cagtgaccgc gtgtatgcca cgtataccgg
     1261 ctcgcaggtt ggcgtgaccg tgcgcgacct caacctcaac cagctcagct gctttggcac
     1321 ggcgttcacc gccagcggca ttgctgctgg tgatgccagc gatctatacc tgacggcgca
     1381 aaaccatatt tatcactaca acgtgaatgg cacgctgatc agcgacatgg ccttcccggt
     1441 cacgtcgatc aattacaccg acgtaaccgt gctggacgat cgggtatttg cctcgtataa
     1501 cggctcgcaa ctgggcttca ccgtgcgtga ccgtaacctg aaccagctgt cgtatgccag
     1561 caccggtttc aacatcaaca gcattgccgc gggcaagagc aataccgtgt atctggcttc
     1621 ggccaatcat ctgtatatct actcggcggc cggctcgttg atttacgaca tgaacttccc
     1681 tgaccacggc gtcaactaca ccggcgtgtc cgcggtgttt ctgcaacaga cctgatatcc
     1741 ggtagcgcac aggcacggca aaagcggcga gggaattctc gccgcttttg ttttgcagat
     1801 gctgcgcgtg tattacatcg ccggcacaaa cacccgcaag cgatggccgt ccgggtcggc
     1861 ggcggtcatg gtcaggccaa agtccatctg cgtcggcggc tgaatcacct ccacgcccag
     1921 gccacgccaa cgcgcccagc aatcgcgcag cgcggcttcg tcggccagcg ccgccgccag
     1981 ctcgacactg ccgggctcgg cggttaccgc cggggccacg ccttcgcgct gccagaggcc
     2041 cagcttcagg ccggacggca gcacaaacat ggcaaaggtg gcctggcatt ccaccggctc
     2101 gatgcccagc agcgattgat aaaaacgcgc gctcaccagc ggatcggcaa cggccagaat
     2161 ggtcatgtcg ggggacagca tgggcaaact ccttgtcaca gtcaggttca gaatcatccg
     2221 cgtcaaacac cgccacacgg ctgtgtttga tgtcgtgact gtgatggtaa tatccccctg
     2281 ctgacagcat ggtgtcagga gtgtgccccc cccatctgac atgccgccgc acacaactca
     2341 cgccgcccgg caacacctca cacacggcgc gccgccagcc gcgccttgcc gccgtgggcg
     2401 catatactcg ctattccgat taccgatgcg tcgccaccgc cagcttgttg catacttgcc
     2461 agtgcgcaca ccctgccacc cagccgcgta tggccgcaga cacgacaacg ccccaagccc
     2521 agccggagcg gctgagccac gccccatctt gaggaatccc catgacccct gccaccgacc
     2581 ccgccctgca gctggcgctg accatgccca aggccgaact gcatgtgcat atcgaaggca
     2641 cgctggagcc cgagctggcc tttgccctgg cccaacgcaa tggcattagc ctgccctacg
     2701 ccgaccaagc cgcgctggcc gccgcctaca actttgacag cctgcaatcg tttctggacc
     2761 tctactacgc ctgcgccgac gtgctgcgca ccgccgacga ttttcgggat ttgatgctcg
     2821 cctaccttga gcgcgccgcc gccgaccata tcatccacac cgaaatcttt ttcgacccgc
     2881 aaacccacac cgcgcgcggc atccccttcg ccaccgtgct ggacggcctg gaagacggcc
     2941 tccagcaagg ccgggcgcgc tggggcatca gcggccggct gatcttgtgc tttttgcgcc
     3001 acctgagcga agaagacgcc ctggccaccc tggccgaagc cgaaccgcac ctggcgcgca
     3061 tcgacggctt tggcctggac tcctcagaac aaggcaaccc gcccagcaaa tttcagcgcc
     3121 tgttcgcccg ctgccgcgca ctgggcaagc cggtggtggc gcacgccggc gaagaaggcc
     3181 cgccggacta catcatccag gcactggacc tgctgggcgt caagcgcatc gaccacggcg
     3241 tgcgcgccag cgaagacccc gccctgctcg cccgcctggc cgccgacggc gtggcgctga
     3301 ccgtctgccc gctctctaac ctgaagctgc gcgtctttcc cgacatgcac gcccacaccc
     3361 tgggccaact gctcgccgcc ggggtgaaag tcaccctcaa cagcgacgac cccgcctatt
     3421 ttggcggcta cctgaacgac aacattcgcg ccgtgcaagc cgcgtttcaa tttgacgccg
     3481 ccacctggaa acaactggcg aaaaacagct ttgaggcgag ctttgccagc gaggcggaaa
     3541 aggcggggtg ggtggagagg gtggaggtgt ggtttggggg gagtgtgcgg ccgaaaagac
     3601 acagctaaat ctgacggcac cgaactgtta gatcaaaaat tagatcaaat gtagaagttg
     3661 caacctagtc ttacaatcca gtgccatcag aacgaggcgc ggccagatcg aatcgcaacc
     3721 tcatactgat cagtacactc ccccactttg gttcatcccg cccaccctct atactgccgc
     3781 tttgttgaga gaggacttga agcatggaac acaaccccta ccgcgacgat gtggtgattt
     3841 cctgggcgga gttgcaccgc gacacccgtc gtttgtgtca tgagttgatg gccaaggggc
     3901 cgttcaaggg cattatcgcc attgcccggg gtgggctgat tccggcggcc ttggtggcgc
     3961 gggagttgga gattcgcttg atcgacacca tttgcgcggc cagttatgaa tccagcgacg
     4021 acgacagcgc gcaaacccag cgcaaggaag tgcgcatcct caagggcatt gagcatgatg
     4081 gcgaggggta tttgctgatt gacgatctgg tggataccgg caacaccgcg cgggtgattc
     4141 gtcagttgtt gcccaaggcg tattttgtca cgctgtacgc caagccgatg gggcgtcagg
     4201 tggtggattt gtgcgtgaag gagttcagcc aggataaatg ggtgtacttc ccgtgggaca
     4261 tcgattatca gtttgtgccg ccgatcaaga agcggctgaa agctgagcag caggggtgat
     4321 gatgaacggg cctgagtcgc gtggcgattc aggcccgttt agcgtggcga acaaaacccg
     4381 cctgnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnngaaatc actcggcgca
     4501 gcgtcaggcg gcttaggcgt tcaggttttt cgacacaatc tcatacacat cgcgcgacag
     4561 gccgtcgtgg gccagcacgc gggccagttc cacgcgcatc agctcggcgc gggccggttc
     4621 gacgcggctg cggcggttaa agcagctgac cagccgcgag gccacttgcg ggttgatggc
     4681 gtcaatcgcc agcacctggt cggccataaa gcggtagccg ctgccgtcgg cggcgtggaa
     4741 ggcggccagg ttgcggccaa aggcgccaat cagcgcgcgc accttgttcg ggttggtcag
     4801 cgcaaaagcc gggtgctcca tcagcgcggt gacggtgcgc agcacgtcct ggcggcggct
     4861 gccggcttgc agggtgaacc atttgtccat caccagggct tcgtcggcaa agcgctgggc
     4921 aaagtcggca aacagggtgt cgcgggcgtg gctgtggctg tcggccagcg ccgccagcgc
     4981 gcccatgcgg tcggtcatat tgtcggcttg ctggtattgc tggctggcca gcgcgccggc
     5041 gtcgccgccg ggcagcagcg ccagatagga cagcgccagg tttttcagcg cgcgccggcc
     5101 ggcgtcttcg tggcggaagg ccaggccgct gtggcggcgc caggcggcgt cccagcgctc
     5161 ggccagcgcc tgcgccagct cggcgcgcgc ctgttcgcgc acctggcaca ggcgggcggg
     5221 gtcggcgtgg tcttgcagct ccagcagctc tacttcggcc ggcagggtca gcgccagcgc
     5281 ggcaaaggcc gggtcgctgt cggcgtggtc cagcagttgg gcaaaggcgt cgatcaggca
     5341 ggccgacagc gtggcctcgc cttcgccccg gtacaggcgc aacagctcgc gggtggccag
     5401 ggtctggccg gcttcccagc ggttgaaggc gtcgctgtca tgggcgcaca aaaaggccag
     5461 ttcttcatcg cggtaggcgt agttcagccg caccggggcc gagaagttgc gcagcagcga
     5521 gggcagcggt ttttcgctca ggccgacaaa ggtaaaggtt tgctcggctt cggtcaccga
     5581 cagcacgcgg gtgagcgggc cgggggcgtc gtcgctggcc agggtaaccg gcagctcgct
     5641 gccgtcggcg gccagcaggc ccaccgccag cggaatgtga aacggcagct tgtcggtttg
     5701 ccctggggtg tccgggcagt gctggcgcac ggtcagcgag gcggcgccgc cttcggcaaa
     5761 ccaatccagg ctgacatcca gcaccggcgt gccggcctgg ctgtaccagc gcttgaactg
     5821 ggtgaggtcg atatggttgg cgtcggccat ggcggcgaca aaatcttcgc aggtcaccgc
     5881 ctggccgtcg tggcggcgga agtacaaatc catgccctga cggaagcccg ccgcgcccac
     5941 cagggtgtgg atcatgcgca ccacttcggc gcctttttcg tacaccgtca gggtgtagaa
     6001 attattcatc tcgatgtagg actcggggcg caccgggtgg gcggtggggc cggcgtcctc
     6061 aggaaactgc aaggcgcgca ggccgcgcgc gtcttcgatg cgcttgaccg cgcggctttg
     6121 catgtctgcc gagaattcct ggtcgcggaa caccgtcagc ccttctttca ggctgagctg
     6181 gaaccaatcc cggcaggtga cgcggttgcc ggtccagttg tggaagtatt catgggcaat
     6241 caccgcctcg acgccttcaa agtcgaagtc ggtggcggtt tcgcggttgg ccagcacaaa
     6301 cttggtgttg aagatgttca ggcctttgtt ttccatggcg cccatattga agtcgccaac
     6361 ggcgacaatc atataggtgt ccaggtcgta ttccaggcca aacacctgct cgtcccactg
     6421 catggatttt ttcagcgagc tcatcgcgtg ggcggttttg tccatgtctg ccggggcgac
     6481 gtaaatctcc agcgtcacct tgcgcccgcc gcgcgtggtg tagtggtcgc gcagcaccga
     6541 caatttgccg gccaccacgg caaacagata ggccggcttg cggtatgggt ccacccattt
     6601 cacccaatgg cgcttgcggt tttcggtcag cccttcgccc actttattgc cattggacag
     6661 cagcaccgga aagcgctggc ggtcggcgat cagcgtggtg gaaaacttgg ccatcacgtc
     6721 cgggcggtcg aggtaatagg tgattttgcg aaagccttct ggctcgcact gggtgtacag
     6781 attgccattg gaggcgtaca ggcccatcag cgaggtgttg gcggccggct ccagccgggt
     6841 ttcgatgtcc agaataaagc tctcgggcac gccgggcagg gtcagcactt catcggccag
     6901 cgtccacgcc gacggcgcca gcgcttcgcc atccagccgc accgccacca gctcggcgct
     6961 gccatccagc tgcagcgtgt cgcaggcttc ggccttgggg ttgcgtttga ccaccatgcg
     7021 cgcacgcacg ctggtgtggc tgtccagaat gtcgaattcc agatcgacgc gatcaatcag
     7081 gaacggggcc ggacggtagt ctttcaggta tttggcgacg ggcgcagaag cggacatggc
     7141 gagaaaaatc caggcaacaa aaggggggag tgcagcggaa aaccgcagac aggccatcta
     7201 gcagacaacg tgccaaccgg ggtttcgtga tgctgatcca ttataacgcc ggcctgacac
     7261 gcccgaaatg catgactgca cagaaatcac cacagggccg ctgccaatcg gccggggcaa
     7321 aaccaccaag cgatgcgcgc gcaccgcgtg gattcctttg ctgcgctagc gtcgcggaca
     7381 caacccagcg cagcggtcct ggctaggcgg gtgaccgcag acaatacgaa agtaccgctc
     7441 cgccacgcca ggcgggttgt gtgagccgcg cttactcgtc cagcagcgca ttcagccgga
     7501 tattggcgat gcgcacaatc tggctcagcg cctcggcaaa ttcgtcttct ggcgcgcgcg
     7561 ccagccgctg gcgcatggcg gccagaatat ccgtccggct caggccggtg acggcgatga
     7621 tgaacggcca gccaaagcgc tggcgatagg ccaggttcag cgcctgcaac tcggcaaatt
     7681 ctgctggggt gcaggcgttc agcccggcgc cggcttgctc gcgggcggaa tccgccgtca
     7741 gctcgccgcg aatggccgcc ttgccggcca gctccgggtg ggcgcacatc agcgccagct
     7801 gttcatcgcg gctggcggcg tgcaaggtgg cggtcagcgc ggcggtgagc gcggcgcggt
     7861 cggcaaacgg gcggcgcgcc caggcgcggc gcatcaccca cggcgaatgc tcgaacaccg
     7921 cgccgacggc ggcaacaaaa ccctcattgt ccagggcgga cagctgagca agagtcaaaa
     7981 aagcaggcat atcagtggcc aggctgtggt tcatgtggct agcctacgaa cttgcccgcc
     8041 ctgccggcaa gcggcggcgg gatataatgc tcttgcccgt ggcgatatat gaccgcactg
     8101 atccgctgca aacgcttcat tccgtgggat tatgcgcaaa ccgccatagt cctcatgcct
     8161 ccccgcccat cggcaagctc cctataatca ggcgcatcga acactgtttt gcgaaagctg
     8221 cccatgggac gcctgaccac ccacgccctt gacaccgccc acggctgccc ggccgccggg
     8281 ctggcctaca ccctgtaccg gctggacgcc gctggcgcgc gccagctgct ggcccacggc
     8341 cacaccaacg ccgacggccg cgccgacgcg ccgctgctgg ccgacgccgc cctgctggcc
     8401 ggggtgtacg aactggaatt tgccgcgggg gattattttc gctgccgggg cgtgaccctg
     8461 cccgagccgc cgtttttgga tgtggtcagc ctgcgctttg gcgtggccga ccccgccggc
     8521 cactaccatg tgccgctgct ggtgtcgccg tgggcctact ccacctaccg gggcagctga
     8581 tggacagcgg atactggctg gaaatcgcct cgctgctgct gcgctggctg cacctgattg
     8641 tcggcatcgc ctggattggc tcgtcgtttt actttgtctg gctggacaac agcctgcgcc
     8701 cgccaaccga cccggccttg cgcgaaaaag gcgtgggcgg cgagctgtgg gcggtgcacg
     8761 gcggcgggtt ttacaaccca caaaaatacc tggtggcgcc caagcaactg cccgacgaac
     8821 tgcattggtt caagtgggaa tcgtattcca cctggctgtc cggttttgcc ctgatcagcg
     8881 tgctgtatta cgcccaggcc gacagctata tgatcgaccc ggccaaggcg gcgctgtcgc
     8941 ccggccaggc ggtggcgctg ggcctggcca ccctggctgg cggctggctg gtgtacgacg
     9001 gcctgtgccg gctgctgatg cgccgctcga tcttgctgtt tgccgcgctg tactttgcct
     9061 ttgtggtggc ggtggcctgg gggctgacgc actggctgtc tggccgggcg gcgtttatcc
     9121 atgtcggcgc catgctggcc accaccatga gcggcaatgt gtttttctgg atcatccccg
     9181 gccagaaaaa aaccgtggcc gccatgcgcc ggggcgacgc gcccgaccca atccacggcc
     9241 agcgcgccaa gcagcgcagc gtgcacaaca actacctcac cctgccggtg ttgttctgca
     9301 tgatctccaa ccactacgcg ctgacctaca accacccgca ggcctggctg gtgctggcgc
     9361 tgattctgct ggccggcgtg ctgatccggc attttttcaa tttacgccac cacggccggc
     9421 tggcctggca ctacccgctg gccggcgtcg cgctgctgct gggcgtgttt atctggctgg
     9481 cgccgaaagc cccaacgcct gccgcacaag gcggcgcccc tgcgcccacc ctggccgaag
     9541 tgcgcccggt gatcgccgcg cgctgcctgg cctgccacgc cagcgcgccc acgctgatgg
     9601 ccgccgcccc ggccgggctg aagctggaca gcgacgaagc gctatacgcc cagcgtcagc
     9661 gcgtggtgga ccaggccgtg cggctaaaag ccatgccgct gggcaacgtc acccaaatga
     9721 ccgacgccga gcgcgcgctg ctggcgcgct gggcagccgg cggctttgcc cgctgagtta
     9781 tccacagccc gattgttatc cacaggcccc gatacccatg cccacctcca ccacccagct
     9841 gattcgcggc cacatcctgc actttttggc cgaccctggc gaacacgacg ccgccgacag
     9901 ctaccaaagc ctgcccgacg gcgcgctgtg gattgtcgat ggccgcgtgg ccgaccttgg
     9961 cccctggccc gaactgcgcg cgcggcgcac ccccgacgaa ctggccgggg cggaatgctt
    10021 tgactacggc cagcacctgg tgctgcccgg cctggtggac acccactgcc actacccgca
    10081 atccggggtg atcgcctcgt ttggccgcca actgctggac tggctgaacg actacacctt
    10141 tcccgccgaa gccgcctttg ccgacccgtg cgtggccgag gccgctgcgg aatattttgt
    10201 cgaacgcctg ctggcgcacg gcaccaccac ggcatcggtg ttcgccaccg tccacccgca
    10261 atcggtagac gcctttatgg ccgccgccga acgccgccag ctgcgcatgc tgtgcggcaa
    10321 agtgatgatg gaccgccacg ccccgccggc gctgtgcgac gacgtcgcct ccgccgagcg
    10381 cgacagcctg gcgctgatcg aacgctggca ccgccgtggc cggctgcgct acagcctgac
    10441 cccacgcttt gcccccacct ccacccccga gcaactggcg ctggccggcg cgctctaccg
    10501 cagccaaccc gacctgcacg tgcaatccca cctggcggaa aaccccgacg aaatcgcctg
    10561 ggtgggccag ctattcccgc agcgccgcga ctacctggac gtctacgccc actacggcct
    10621 gctcggcccg cgcaccatct acggccactg catccacctc gccccagaag aaatccgcga
    10681 catggcgcac accggcaccg ccgccgcctt ctgccccacc tcca
//
LOCUS       DAOPYX010000067        10710 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_7112_length_10710_cov_11.043172, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000067 DAOPYX010000000
VERSION     DAOPYX010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 10710)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10710)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10710
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7112_length_10710_cov_11.043172"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(5..649)
                     /locus_tag="PKN55_05060"
     CDS             complement(5..649)
                     /locus_tag="PKN55_05060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpW family outer membrane protein"
                     /protein_id="HNX70646.1"
                     /translation="MKKIALALCAGAALASSAAMAAQGDILARFRVINVNPDVKTTGL
                     LASPLSAGVQDDTIPELDFTYMITNNVGAELILGTSRHSVTANRTALGGGNGSIGKVS
                     VLPPTLTLQYHFNPEGAIRPYVGAGLNYTRFYRAGGLSTEVGGVSIKKNSFGPALQVG
                     MDVPVAKNVFLNVDLKKIWIDTKASGNSGAINGVDLGRLEINPLVFGVGVGMKF"
     gene            987..2510
                     /gene="ubiD"
                     /locus_tag="PKN55_05065"
     CDS             987..2510
                     /gene="ubiD"
                     /locus_tag="PKN55_05065"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HNX70647.1"
                     /translation="MKYRDLRDFIAQLQAMGELRRIDAPVSTRLEMTEICDRVLRAGG
                     PALLFTRPQGHSMPVLGNLFGTPRRVALGMGAEEVSALRDIGKLLSYLKEPEPPKGLK
                     DLWDKWPVFKNVLNMAPKTIRHAPCQEEVLEGEQVDLGRLPVQHCWPGDVAPLITWGL
                     TVTRGPHKKRQNLGIYRQQVIGRNQVIMRWLAHRGGALDFREHKRAHPGQPFAVSVVL
                     GCDPATILGAVTPVPDTLSEYQFAGLLRGDRTELTQCLGNELTVPAYAEIVLEGRLTP
                     VEQGFTGVSEHGVPLKEQEGFLAALEGPYGDHTGYYNEQDWFPVFTIDRITRRREAIY
                     HSTYTGKPIDEPAVLGVALNEVFVPILQKQFPEIVDFYLPPEGCSYRMAVVSIKKQYP
                     GHAKRVMMGVWSFLRQFMYTKFIVVVDEDINTRDWNEVIWAITTRMDPVRDTVLVENT
                     PIDYLDFASPISGLGGKMGLDATNKHPGETSREWGTPIVMDTAVKARVDAMWGELGL"
     gene            complement(2580..3380)
                     /locus_tag="PKN55_05070"
     CDS             complement(2580..3380)
                     /locus_tag="PKN55_05070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011134058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HNX70648.1"
                     /translation="MSALMLLALAGMGVVAGLLAGLLGVGGGLVLVPVLVWVMEGSGM
                     HAHVQHMALGTSLAVMVFTSFSSVRAHHRHGAVDWAIVRRFAPPIVLGTLLGTQLAGL
                     LPSRELRWFFIVYAYLIAAQMLLDAKPKASRTLPGAVGLGGVGLGIGVVSSFVGIGGG
                     SMSVPFMSWCNIPLKRAIATSAALGWPIAFSGALGYMASGWGRGDLPAYTVGFVYLPA
                     VATLCLITMATAPMGARLAHRLPVAKLKKLFALLMVVMASQLLIKALA"
     gene            complement(3377..3988)
                     /locus_tag="PKN55_05075"
     CDS             complement(3377..3988)
                     /locus_tag="PKN55_05075"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008952035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HNX70649.1"
                     /translation="MADAPELDLPARKTALRAELRRRRLALSPRYRRQAALALARHCR
                     RWLRREHTLAAYLSHGSEIDTRPLLSAARARGCRVLLPVVPARGRRLRFTALDARPRW
                     RANRYGIPECLGPRVPLSRATHVLLPLLGFDAAGRRLGQGGGFYDATLAALRARPGRA
                     KLIGLAYAAQRLAEVPCEAWDQRLDAVVTEHGVSRVWTAQEAA"
     gene            complement(4047..4226)
                     /gene="ssrS"
                     /locus_tag="PKN55_05080"
     ncRNA           complement(4047..4226)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="PKN55_05080"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(4248..4568)
                     /locus_tag="PKN55_05085"
     CDS             complement(4248..4568)
                     /locus_tag="PKN55_05085"
                     /inference="COORDINATES: protein motif:HMM:NF017016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HNX70650.1"
                     /translation="MSQVHIEVNLLGRRFAVGCPKEEQASLVEAVALLEQRMAEVRDV
                     GRVVEVDKIAIIAALNLANDLLKLQKNPPPSASDLDIEEIQRKIDAMNDTIDQAMLEQ
                     NRMF"
     gene            complement(4565..4765)
                     /locus_tag="PKN55_05090"
     CDS             complement(4565..4765)
                     /locus_tag="PKN55_05090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX70651.1"
                     /translation="MDAELDLLHGKILQLAELCRQLRLDNNQLRDRLAEREVENRDLK
                     YKVEETRARIENLLARLPEGTQ"
     gene            complement(4863..5384)
                     /gene="cobU"
                     /locus_tag="PKN55_05095"
     CDS             complement(4863..5384)
                     /gene="cobU"
                     /locus_tag="PKN55_05095"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006484041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HNX70652.1"
                     /translation="MRQFFIGGARSGKSRLAEAEALAWPGEVIYLATAKVGDEEFAQR
                     VAAHRARRPAQWGLIEAPASLAQALNAAAAPERLLLVDCLTLWLVGFLTADGVDEARW
                     RQERAALLQLLPQLPGKIVLVSNEIGWGVVPMGAATRWFVDELGWLNQAVAACCEQVT
                     LVAAGLPLVLKSS"
     gene            5471..6499
                     /gene="cobT"
                     /locus_tag="PKN55_05100"
     CDS             5471..6499
                     /gene="cobT"
                     /locus_tag="PKN55_05100"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012698665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HNX70653.1"
                     /translation="MAVLHPDAGLAARQRQSQLTKPAGSLGRLEALACWLAERSGQVC
                     PPLPQPGIAVFAGDHGVAADGVSAFPSAVTAEMVKNFTRGGAAINVLARAIAAPLEVV
                     DVGVASPLPAELPIVHARVRAGTGNLRTEAAMSLDECRQALAIGRASARRLHAQGATL
                     LIAGDMGIGNTTASACLVCAFTGATPEEIVGLGTGIDDARRAHKVTVVSEALARAQTA
                     GAHDGESWLAQVGGLEIAAMAGLLLEATALGIPALLDGFIATAAALAARAIEPAAADW
                     WLASHRSQEQGHLRALTALGLTPLIDLELRLGEGSGAALAVPLIQMAVRLHNEMATFA
                     EAGVSGAA"
     gene            6496..>6692
                     /locus_tag="PKN55_05105"
     CDS             6496..>6692
                     /locus_tag="PKN55_05105"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HNX70654.1"
                     /translation="MKPFTVTLLRHGDTPLSGRLIGQRSDPPPTAAAQVQLAATLAQL
                     HARQPITRLAASPLLRCRAPA"
     assembly_gap    6693..6792
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            6919..7683
                     /gene="cobS"
                     /locus_tag="PKN55_05110"
     CDS             6919..7683
                     /gene="cobS"
                     /locus_tag="PKN55_05110"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012698657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HNX70655.1"
                     /translation="MRELILAVQFLTRLPTPQLRDFDPAWLAGCGRWFAPVGALIGAL
                     LLLPWQPLAQVEPWLAAALTLLLWVWLTGALHLDGLADLADGLGAAHRDPARFLAVLK
                     DPHVGSFGVVALVMALLLKLVGLMLAARHGLSPWALLLLPAWARFGALWWSVSLPPLA
                     SGGQAERFAWKSGPALLWLTLLGLLAISAWLAPWLCLAGPLALWLWRRFLLTRLGGVT
                     GDCLGAGIECVETGLLLAGVGWQLSGAPAWLVSLMG"
     gene            complement(7808..8407)
                     /locus_tag="PKN55_05115"
     CDS             complement(7808..8407)
                     /locus_tag="PKN55_05115"
                     /inference="COORDINATES: protein motif:HMM:NF012409.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 19 protein"
                     /protein_id="HNX70656.1"
                     /translation="MLTAAHLQALGLDASWLDPLNATFDRFAINTPQRMAAFIGQCGH
                     ESAGFHTLNENLNYSAQGLRNTWPKRFPDDATANAYARQPEKIANKVYADRMGNGNEA
                     SGEGWRYHGRGLIQLTGKANYQAAGTALGVDLVSSPDQVAAPEYAALTAGWFWDSHKL
                     NALADKQDNTGITKAINGGTLGLDDRVARTNKALAVLKG"
     gene            8732..9799
                     /gene="hemE"
                     /locus_tag="PKN55_05120"
     CDS             8732..9799
                     /gene="hemE"
                     /locus_tag="PKN55_05120"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_043595575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HNX70657.1"
                     /translation="MTALQNDVFLRALLREPVPYTPIWMMRQAGRYLPEYCETRRRAG
                     NFMGLCTNPTLATEVTLQPLERFPLDAAILFSDILTVPDAMGLGLYFAEGEGPKFERP
                     LTDEASILALAAPDPTDRLRYVLDAVSSIRQALNGRVPLIGFSGSPWTLACYMVEGGS
                     SADFRKVKALMYDRPDLMHHILQVTTRAVIDYLNAQIEAGAQAVMIFDTWGGALSHAA
                     YETFSLAYMQEIVSQLTREREGRRVPSIVFTKNGGQWLEKIADIGSDAVGLDWTTDIG
                     DARRRVGARVALQGNFDPNALFANPAAIEAEAARILAAYGHGSGHVFNLGHGISQFTP
                     PEHAGVLVEAVHRLSRQYHAA"
     gene            9848..>10710
                     /locus_tag="PKN55_05125"
     CDS             9848..>10710
                     /locus_tag="PKN55_05125"
                     /inference="COORDINATES: protein motif:HMM:NF018184.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HNX70658.1"
                     /translation="MSRHPAARQRQQRHVHAVLRHIDRHLDQPLDLVALAAVAHVSPF
                     HFHRVFRAWTGENLGDYLSRRRLTLAACWLCNEPAASVLTLALRAGFASGEAFSRAFK
                     ARFGLSPSQWRGASAAKRQELRNLDQAAQVALRHDGPVFSDLEPDMHVTLCQRPAVTV
                     AYLRHTGPYGQAIAEFWRDQFVPWLHAQALLPYAKYGIGHDNPQLTDPAQCRYDACVA
                     LPPGYAPPADMLTMTIPGGLYAVYDFRGGVPEIAQAWTALFAEWLPASGWGVDARPCF
                     EFYPEDAFFDPA"
BASE COUNT         1790 a         3497 c         3488 g         1835 t
ORIGIN      
        1 gttcttagaa cttcatgcca acgccaacac cgaacaccag cgggttgatt tccaggcgac
       61 ccaggtccac gccattgatg gcgccgctgt tgccggaagc cttggtgtcg atccagatct
      121 ttttcaggtc aacgttcagg aacacattct tggcaaccgg cacgtccata cccacttgca
      181 gggccgggcc gaagctgttt ttcttgatgg acacgccacc cacttcagtg ctcaggccac
      241 ccgcgcggta gaagcgggtg tagttcaggc cggcgccaac gtacgggcga atcgcgcctt
      301 ccgggttgaa gtggtattgc agggtcaggg tcggcggcag cacgctgacc ttgccgatgg
      361 agccattgcc gccgcccagg gcggtgcggt tggcggtgac gctgtgacgc gaggtgccca
      421 ggatcagttc ggcgcccacg ttgttggtga tcatgtaggt gaagtcgagc tcggggatgg
      481 tgtcatcctg cacgccagcg ctcagcgggg aggccagcag accggtggtc ttgacgtccg
      541 gattgacgtt gatcacgcga aagcgagcaa ggatgtcgcc ttgggcggcc atggcggcgg
      601 aagaagcgag ggctgcgcca gcgcacagag ccaatgcgat ttttttcatg gttttctctc
      661 ctgatgttcc cgatatcaaa cggtgtgttt ggggttaccc tgtcatggcc cggcagatgg
      721 cgttgcctcc ggctgtcgtt tggctgctgc cacacccccc atgcttaggt catgaatgct
      781 agcaaaagaa tgcccttgca ttcttgatct gacgcaaatt aacgccctgc gccgtcggca
      841 aactgcaaca ctgtctgaaa aaaaccacac tgacagtagc ggaattgccg ccgcgcgcca
      901 caatccgcca gcctgcgcag ggcagcagca gcgccccggc tttctgctag actcgcgcct
      961 tgtcctgcac aaaacgagtt gcccccatga aataccgcga tctgcgcgac ttcatcgccc
     1021 aactgcaagc catgggcgaa ttgcgccgca ttgacgcgcc ggtgtccacc cggctggaaa
     1081 tgaccgaaat ctgtgaccgc gtactgcgcg cgggcggccc ggcgctgctg ttcacccgcc
     1141 cgcaaggcca cagcatgccg gtgctgggca atctgtttgg cacgccgcgc cgggtggcgc
     1201 tgggcatggg cgccgaagaa gtgtcggcgc tgcgcgacat cggcaagctg ctgtcctacc
     1261 tgaaagaacc cgagccgccc aaggggctga aagatttgtg ggacaaatgg ccggtgttca
     1321 aaaacgtgct gaacatggcg ccaaaaacca tccgccatgc gccctgccag gaagaagtgc
     1381 tggaaggcga gcaggttgat ctgggccgct tgccggtgca gcactgctgg ccgggcgatg
     1441 tggcgccgct gatcacctgg ggcttgaccg tcacgcgcgg gccgcacaaa aagcgccaga
     1501 acctggggat ttatcgccag caggtgattg gccgcaatca ggtgatcatg cgctggctgg
     1561 cgcatcgcgg cggcgcgctg gattttcgcg agcacaaacg cgcccacccc ggccagccgt
     1621 ttgccgtgtc ggtggtgctg ggctgcgacc cggccaccat tctgggcgcg gtcacgccgg
     1681 tgccggacac cttgtcggaa tatcaatttg ccggcctgtt gcgcggcgac cgcaccgagc
     1741 tcacccagtg cctcggcaat gagctcaccg tgccggccta cgccgaaatc gtgctggaag
     1801 gccggctgac cccggtcgag cagggcttta ccggggtgag tgaacatggc gtgccgctga
     1861 aagagcaaga aggctttctg gcggcgctgg aaggcccgta tggcgaccac accggctatt
     1921 acaacgagca ggactggttt ccggtgttca ccatcgaccg catcacccgc cgccgcgagg
     1981 cgatttacca cagcacctac accggcaagc cgattgacga gccggcggtg ctgggcgtgg
     2041 cgctgaacga ggtgttcgtg cccatcctgc aaaagcagtt tccggaaatc gtcgattttt
     2101 acctgccgcc agaagggtgc agctaccgca tggcggtggt cagcatcaaa aagcaatacc
     2161 ccggccacgc caagcgggtg atgatggggg tgtggagctt tttgcggcag ttcatgtaca
     2221 ccaagttcat cgtggtggtg gacgaggaca tcaacacccg cgactggaac gaagtgatct
     2281 gggcgatcac cacgcgcatg gatccggtgc gcgacactgt gctggtggaa aacaccccga
     2341 ttgactatct ggacttcgcc agcccgattt ccgggctggg cggcaagatg gggctggacg
     2401 ccaccaacaa gcaccccggc gaaacctcgc gcgagtgggg cacgccgatt gtgatggata
     2461 ccgcagtcaa ggcgcgggtg gacgccatgt ggggcgagtt gggcctgtaa acccgcccca
     2521 aacggcagag cctgatgggc agggggcaat gcaccagccc accaggcggg gagacgacgc
     2581 taggccaggg ctttgatcag caattggctg gccatcacca ccatcagcaa ggcaaacagc
     2641 tttttcagct tggccaccgg cagccggtgc gccagccgcg cgcccatcgg cgcggtggcc
     2701 atggtgatca ggcatagcgt ggccaccgcc ggcaaataca caaagcccac ggtgtaggcc
     2761 ggcaaatcgc cgcgccccca gccgctggcc atatagccca gcgcgccaga aaacgcgatg
     2821 ggccagccca gcgccgcgct ggtggcgatg gcgcgcttga gcggaatatt gcaccagctc
     2881 atgaacggca cgctcatcga gccgccgccg atgccgacaa aactcgacac cacgccaatg
     2941 cccagcccca cgccgcccag gcccaccgcg ccgggcaggg tgcggctggc cttgggtttg
     3001 gcgtccagca gcatttgcgc ggcaatcagg taggcgtaaa caataaaaaa ccagcgcagc
     3061 tcacggctgg gcagcagccc ggccaactgc gtgcccagca gcgtgcccag cacaatcggc
     3121 ggggcaaagc ggcgcacaat cgcccaatcc accgcaccat gccgatgatg ggcgcgcacc
     3181 gaagaaaaac tggtgaacac catcaccgcc agcgaggtgc ccagcgccat atgctgcaca
     3241 tgggcatgca tgccgctgcc ttccatcacc cataccagca ccggcaccaa caccaggccg
     3301 ccgcccacgc ccagcaagcc agccaacagc ccggcgacca cgcccatgcc ggccaaggcc
     3361 agcaacatca atgcgctcat gcggcctcct gcgcggtcca tacccggctg acgccatgtt
     3421 cggtaaccac ggcgtccaga cgctggtccc acgcttcgca cggcacttcc gccagccgct
     3481 gcgcggcata agccaggcca atcaatttgg cgcggcctgg ccgcgcgcgc agcgccgcca
     3541 gggtggcgtc gtaaaatccg ccgccctggc ccaagcggcg gccggcggcg tcaaagccca
     3601 gcaagggcag cagcacatgg gtggcgcggc tgaggggcac gcgcgggccc aggcattcgg
     3661 ggatgccata gcggttggcg cgccagcgcg ggcgggcgtc cagcgcggta aagcgcagcc
     3721 gccggccgcg cgccggcacc accggcagca gcacgcggca gccgcgcgcg cgcgcggcgc
     3781 tcagcaaggg acgggtatcg atttcgctgc cgtggctcag ataggcggcc agggtgtgtt
     3841 cgcggcgcag ccagcgccgg cagtggcgcg ccagcgccag cgctgcctgg cggcggtagc
     3901 gcggcgacaa ggccagccgg cggcggcgca gttcggcgcg cagggcggtt ttgcgggcgg
     3961 gaagatcaag ctcaggcgca tcggccatgg ggcgcgctcc agaaacgaaa acgccttgtg
     4021 tgagcaactc acacaaggcg tggatagggt cccccgcaga tgccgatccg gccggtcatc
     4081 ctgaaccttg cggatcaggg ggtcgccggc tttgcctgtt gggcgcgccg gacaagcggc
     4141 gcgacacgct ggggcaatgg ggctggcaac cagttaccaa ctattggttc aaggaattta
     4201 agccgttccg aacaccgcag gggagaaaga actttgacga acgtcggtca aaacatgcga
     4261 ttttgctcta acatggcttg atcaatcgtg tcgttcatgg cgtctatttt acgctggatt
     4321 tcttcaatgt ccaagtctga tgctgaaggc ggcggattct tctgtaattt caagaggtca
     4381 ttggccagat tcagcgcggc gatgatggca attttgtcca cttccaccac gcggccgacg
     4441 tcgcgcacct cggccatgcg ctgttccagc agcgccaccg cctcaaccag cgaggcttgt
     4501 tcttctttgg ggcaacccac ggcaaagcgc cgtcccagca ggttgacctc gatatgcacc
     4561 tggctcattg cgtcccctcg ggcagtcgcg ccagcaggtt ttcgatgcgg gcgcgggttt
     4621 cctcgacctt gtatttcagg tcacggtttt ccacctcgcg ttcggccaga cggtcgcgca
     4681 gctggttgtt gtccaggcgc aactgtcggc acagctcggc gagctggagg attttgccat
     4741 gaaggagatc gagttctgcg tccatggttg gcactataac gggccgctgc tgatagcgtc
     4801 aaaccacgac gaagagaagc gcaaaaaacc cataggcata aattgaccgt attttctttc
     4861 cgtcaggatg atttcagcac cagcggcaag ccagccgcca ccaaagtcac ctgctcgcag
     4921 caggcggcca ccgcctgatt gagccagccc agttcatcga caaaccagcg ggtggcggcg
     4981 cccatcggca ccacgcccca gccgatttca ttgctgacca gcacaatctt gccgggcaac
     5041 tgtggcaaaa gctgcaacag cgccgcgcgc tcttggcgcc agcgcgcttc gtccacgcca
     5101 tcggcggtga gaaaacccac cagccacaac gtcaggcaat ccaccagcag caggcgctcg
     5161 ggcgcggcag cggcgttcag cgcctgggcc aggctggccg gggcttcgat caagccccat
     5221 tgcgccggcc ggcgggcgcg gtgcgcggcc acacgctggg caaattcttc gtcgcccact
     5281 ttcgcggtgg ccagataaat cacctcgccg ggccaggcca gcgcctcggc ctcggccagc
     5341 cggcttttgc cgctgcgcgc gccgccaata aaaaactgtc gcatgctggg cctttctgtg
     5401 cgccaaagct tggcccggca ttatagggct ccggtagaat ctgcggcttt gcctgatcgg
     5461 aattgttctc atggctgtct tgcaccccga cgccggcctg gccgcgcgcc aacgccaatc
     5521 gcaactgacc aaacccgctg gcagcctggg ccggctggag gcgctggcct gctggctggc
     5581 cgaacgcagc ggccaggtgt gcccgccgct gccgcagccc ggcattgccg tgtttgccgg
     5641 cgaccacggc gtggcggccg atggcgtgtc ggcctttccc agcgcagtca ccgccgaaat
     5701 ggtgaaaaac ttcacccgtg gcggcgccgc catcaatgtg ctggcgcgcg cgattgccgc
     5761 cccgctggaa gtggtggacg ttggcgtggc cagcccgctg ccggccgaac tgccgattgt
     5821 gcacgcccgc gtgcgcgccg gcacgggcaa tctgcgcacc gaagccgcca tgagtctgga
     5881 cgaatgccgc caggcgctgg ccattggccg cgccagcgcc cgccggctgc acgcccaggg
     5941 cgccacgctg ctgattgccg gcgatatggg tattggcaac accaccgcct cggcctgcct
     6001 ggtgtgcgcc ttcactggcg ccacgccaga agagattgtc ggcctgggca ccggcatcga
     6061 cgacgcccgc cgcgcccaca aggtgacggt ggtgagcgag gcgctggcgc gcgcgcagac
     6121 ggccggcgcg cacgatggtg aaagctggct ggcgcaggtt ggcgggctgg aaatcgccgc
     6181 catggccggc ctgctgctgg aagccaccgc gctgggcatc cccgccctgc tggatggctt
     6241 tatcgccacc gccgccgcgc tggctgcccg cgccatcgag cctgccgccg ccgactggtg
     6301 gctggccagc caccgctcgc aagagcaggg ccatctgcgc gcgctgaccg cgctgggcct
     6361 gacgccgctg attgacctgg aattgcgcct gggcgaaggc tcgggcgcgg cgctggccgt
     6421 gcccctgatc cagatggcgg tgcgcctgca caatgaaatg gccacctttg ccgaagctgg
     6481 cgtctctggc gcggcatgaa gcccttcacc gtcaccctgc tgcgccacgg cgacacgccc
     6541 ttgagcgggc ggctgattgg ccagcgcagc gacccgccgc ccaccgccgc cgcgcaagtg
     6601 caactggccg ccaccctggc gcagctgcac gcccgccagc ccatcacccg gctggccgcc
     6661 tcgcccctgc tgcgctgccg cgcgccggcc gannnnnnnn nnnnnnnnnn nnnnnnnnnn
     6721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6781 nnnnnnnnnn nntgctggcc gaggcgatgg ccatgccctg gccgctggcg cggcgggtgg
     6841 cggtggcgcg cggcggctgg ctgcgcctgt cttgctatcc gccagagccg gcctggctgc
     6901 tggcgctgga gggtgcggat gcgtgagctg attttggccg tgcagtttct cacccgtctg
     6961 cccaccccgc agctgcgcga cttcgacccg gcctggctgg ccggctgcgg ccgctggttt
     7021 gccccggtgg gcgcgctgat tggcgcgctg ctcttgctgc cctggcagcc gttggcccaa
     7081 gtggagccct ggctggcggc ggcgctgacc ttgctgctgt gggtgtggct gaccggcgcc
     7141 ctgcatctgg atggcctggc cgacctggcc gacggcctgg gcgcggcgca ccgcgacccg
     7201 gcgcgctttc tggcggtgct gaaagacccg catgtcggca gttttggcgt ggtggcgctg
     7261 gtgatggcct tgttgctcaa gctggtgggg ctgatgctgg cggcgcgcca tggcttatca
     7321 ccgtgggcgc tgctgctgtt gccggcgtgg gcgcgctttg gcgcgctgtg gtggtcggtc
     7381 agcctgccgc cgctggccag cggcggccag gcggaacgct ttgcctggaa aagcggcccg
     7441 gcgcttttgt ggctgaccct gctgggcctg ctggctattt cggcttggct ggcgccgtgg
     7501 ctgtgcctgg cggggccgct ggcgctgtgg ctgtggcgac gctttttgct tacccgactg
     7561 ggtggggtga ccggggattg cctgggcgcc ggcattgagt gcgtggaaac cggcctgctg
     7621 ctggccggcg tgggctggca gctgtcgggc gcgccggcgt ggctggtcag cctgatggga
     7681 taaggccgcg caatgcctgg ggctgccgcc gtagggcttg gcggcgttag cgaagccatc
     7741 ctggcgaggg gcatcaataa cggcaagatg cccccagcac gccaggaaag tttggtgggc
     7801 gctaacctta gcccttcagc accgccagcg ccttattggt gcgcgccacc cggtcgtcca
     7861 gccccagcgt gccgccattg atggccttgg tgatgcctgt gttgtcctgc ttgtcggcca
     7921 gcgcattcaa cttgtgagag tcccaaaacc agccagcggt cagcgccgcg tattccggcg
     7981 cagcaacttg gtccgggctg gagaccagat ccacccccaa ggccgtgcct gctgcctgat
     8041 agttggcctt gccggtcagc tggatcaggc cacggccgtg atagcgccag ccttcgcccg
     8101 aggcttcgtt gccgttgccc atgcggtcgg cgtatacctt gttggcgatt ttttccggct
     8161 ggcgggcgta ggcgttggcg gtagcgtcgt caggaaagcg cttgggccag gtgttgcgca
     8221 ggccctgggc cgagtagttg aggttttcgt tcagcgtatg aaacccggca gattcatggc
     8281 cgcactggcc gataaaggcc gccatgcgtt gcggggtgtt gatggcaaag cggtcaaaag
     8341 tggcgttcag cgggtcaagc cagctggcgt ccaggcccag ggcctgcaaa tgagcagcgg
     8401 tcagcatagt gaaaattctc cggaaaaata aggctgtcag ataagcccaa tgcggtgtgt
     8461 gccgccgtgc tgcgctgccg gcgcgcgtct tgtcctgaac gcttgcgcca cagggtttgt
     8521 gagtcatgcc gccaccacac gggggtgctg cgcgcgcaag cacgcttgac gcaaacagca
     8581 tccgctggac aggctgatgg cataagccgc cccgagtcaa gcaaacgaca tgaaacctca
     8641 tgccctgtgt ggataaagtg tgtgcttcac accgcgccga cctggcgcag ccgcgtataa
     8701 tcccggcatg attctgacct gttgagctgc catgaccgca ctgcaaaatg acgtcttcct
     8761 gcgcgcgctg ctgcgcgagc cggttcctta caccccgatc tggatgatgc gccaggccgg
     8821 ccgctacctg ccggagtact gcgaaacccg ccgccgcgcc ggcaatttca tgggcctgtg
     8881 caccaacccg acgctggcca ctgaagtcac cctgcaaccg ctggagcgct ttccgctgga
     8941 tgcggcgatt ttgttttccg acatcctcac cgtgccggac gccatgggcc tggggttgta
     9001 ctttgccgaa ggcgaaggcc cgaagtttga gcgcccgctg accgacgaag cctccatcct
     9061 ggcgctggcc gcccctgacc ccaccgaccg cctgcgctat gtgctggacg cggtgagcag
     9121 catccgccag gcgctgaacg gccgcgtgcc gctgattggt ttttccggta gcccgtggac
     9181 gctggcctgc tatatggtgg aaggtggctc gtcggcggat ttccgcaagg tcaaggcgct
     9241 gatgtacgac cgcccggacc tgatgcacca catcctgcaa gtgaccaccc gcgcggtgat
     9301 cgactacctg aacgcgcaaa tcgaagccgg cgcgcaggcg gtgatgattt tcgacacctg
     9361 gggcggcgcg ctgtcgcacg cggcgtatga aaccttctcg ctggcctata tgcaagagat
     9421 cgtcagccag ctcacccgcg agcgcgaagg ccggcgcgtg ccgtccatcg tgttcaccaa
     9481 gaacggcggc cagtggctgg aaaaaatcgc cgacatcggc agtgacgccg tcggtctgga
     9541 ctggaccacc gacattggcg acgcccgccg tcgcgtgggc gcgcgcgtgg cgctgcaagg
     9601 caatttcgac ccgaacgcgc tgtttgccaa cccggccgcc atcgaagccg aagccgcgcg
     9661 catcctggcc gcctacggcc acggcagcgg ccatgtgttc aacctggggc acgggatttc
     9721 ccagttcacc ccgccggaac acgccggggt gttggtggag gcggtgcatc gtctgtcgcg
     9781 gcagtatcac gccgcctaag gacagccgct cacacactgc cttggtgggg tgtgggcgcg
     9841 ccaagccatg tcccgtcatc ccgccgcgcg ccagcgtcag caacggcatg tgcacgccgt
     9901 gctgcgccat attgaccgcc acctcgacca gccgctggat ttagtcgcgc tggcggcggt
     9961 ggcgcatgtg tcgccgtttc attttcatcg ggtgtttcgc gcctggacgg gcgagaatct
    10021 gggcgactac ctcagccgtc gccggctgac gctggcggcc tgctggctat gcaacgaacc
    10081 ggccgcctcg gtgctgacgc tggcgctgcg cgccggcttt gcctcgggcg aggcgttcag
    10141 ccgcgccttc aaggcgcgct ttggcctcag ccccagccag tggcgaggcg ccagcgccgc
    10201 caaacggcaa gaattgcgca atctggatca ggcagctcag gtcgcgctgc ggcatgatgg
    10261 accggtcttc tctgacctgg agcccgatat gcacgtcacc ctttgccaac gcccggccgt
    10321 caccgtggcc tacctgcgcc acaccggccc ctacggccaa gccatcgccg agttttggcg
    10381 cgaccagttt gtgccctggc tgcacgccca agcattgctg ccctacgcca aatacggcat
    10441 cggtcacgac aacccgcaac tgaccgaccc ggcgcaatgc cgctacgacg cctgcgtggc
    10501 cctgccgcca ggctatgcgc caccggcgga catgctgacc atgacgattc cgggcggttt
    10561 gtacgcagtg tatgattttc gcggcggcgt gcccgagatt gcccaggcct ggacggcctt
    10621 gtttgccgag tggctgccgg ccagcggctg gggggtggat gcgcggccgt gttttgagtt
    10681 ttacccggaa gacgcgttct tcgacccggc
//
LOCUS       DAOPYX010000068        10700 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_7126_length_10700_cov_9.692344, whole genome shotgun sequence.
ACCESSION   DAOPYX010000068 DAOPYX010000000
VERSION     DAOPYX010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 10700)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10700)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10700
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7126_length_10700_cov_9.692344"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(120..1079)
                     /locus_tag="PKN55_05130"
     CDS             complement(120..1079)
                     /locus_tag="PKN55_05130"
                     /inference="COORDINATES: protein motif:HMM:NF012885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX70659.1"
                     /translation="MPLAIEQLIADFGGVARLATLLAEHFPAEPVSRGAIYKWRERGS
                     LPLAQAERLRVLAQRLGRGFELSDYQRGQAASPAPVTALRVLDVSLAQAPAGVHASWL
                     AALDALGLAGVCLGDALGAPRALLAHCHTPPWLEAPAQEMAVWLAAQAFAGRRQGGVR
                     LCLDAHPPDADELRRCLRLLDALGLRAALRLPVRAEWPADWLHALRQATALGADSLTL
                     DDAGLHWVPEQWAETLRRLRARLGSEVCLGARCHNRHGLALANALAALSAGARQLDGA
                     LSGEHAAPLPALAATLDIHAARYPVRLSVPAQALARAQALHMH"
     gene            complement(1351..5826)
                     /locus_tag="PKN55_05135"
     CDS             complement(1351..5826)
                     /locus_tag="PKN55_05135"
                     /inference="COORDINATES: protein motif:HMM:NF017081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNX70660.1"
                     /translation="MKGVLHTWLWLLGLALAYFASGQLGLTVPYLGAFVSLIWPPTGV
                     AVAALWRGGLSRWPGVWLGSLAINLASGGTPLMSVAIACGNTLAPLLTVWALRRVGAS
                     LHFDTRRDMNHYLLAISGGMLASASVGTLSLWLSGVMQPAQLPKIFLIWWAGDAVGAL
                     LLGIPLLTFVPRLWRELSPTRRLEGLGCLAGMLMIGQLLLYSPSHAALLPLVFIPFLL
                     LSWLAVRTGLMLSSLAVLLLSALMAWGTVQGMGPFHRPSVHEGLALLWGYMATLMVVA
                     LLLSALVAELSANERRWQFALEGADTGVWDWHARSQRIQYSPRWRMLLGVSAAELGNK
                     LTGWETRVHPDDLPAFIAVLAGREQPASSHELRPHGSEIRLRAGNGQWRWYGLRSQVV
                     SRDRDGAPSRVIGTLTDITRYKDTLYWLKLLERALHSARNGVIISDVRQPDQPIIYVN
                     DAFERTTGYRREEVLGRNCRMLNQGDREQPGLDALRTAMAAGKSCQTVLRNYRKDGSL
                     FWNELSIAPVYDERQQLTHYIGVQTDISAQILAQERLSARDKLLHKLSEQAPGLICQM
                     HMDGKRLSLPYASEAIREVFGLSPEDVREDASPLCRVLHPDDRLRLYRGLHQAARQQA
                     PWRDEFRVELQPGQPEWREAHAMPEALEDGSVLWYGYVADISERKAAMLKLEEGSAFY
                     QAMFETNNAVKLLVDPARKQVVDANPAASAFYGYPHEVLVGMPISQINTATPEHIRAA
                     LACAQATGQQRFEFVHRLADGSTRDVEVYSGPIRLGGRELLFSIIHDISERKRAEEQL
                     RFTASVFEHAHEGIMITDANAQIVDVNRTFTEITGYAREDVLGRSPNLLRSGYHSQAF
                     YSEMWAALYTEGYWRGEVWNRRKNGEVYPELLTISSVRPPDGRVTHYVAVFSDITVLK
                     EHQQKLERMAHYDALTQLPNRVLLADRLMLALTQARRSGKLLAVCYLDLDGFKPVNDT
                     FGHEAGDALLVEVASRLKQVLRNGDTVARLGGDEFVLLLSGLDDIGKCQQALDRVLHT
                     IAQPYQLGKERLANLSASIGVTLYPVDGADADTLLRHADQAMYLAKQAGRHRYHLFDP
                     EHDRQAHAHREAVARIEAALNAGEFRLYYQPKVNMRHGVVVGAEALIRWQHPERGLLP
                     PGEFLPIIEDSEFSVELGEWVMEEALRQMAVWRAQGLTLPVSVNISARHLQHPQFSQR
                     LHTLLARHPDTPPGQLELEVLETAALDDIAYVSALMERCRALGVMFALDDFGTGYSSL
                     TYLKRLPANVLKIDQSFVRDMLKDPEDLAIIEGVIGLTQAFRRTAIAEGVETLEHGAL
                     LIQLGCDLGQGYGIARPMPAEAVPGWVAGFSAPESWRRAGLHPWSRDDFPLLGASIDH
                     QHWIEELVQCLEQDASHCPPPLDIHHCRFGRWYHGPGQDRYGQLPAFRVIDASHHQVH
                     ELASQLHAAHLAGELARVRTGIPELYALRDQMLAQLDALRRVVASRSGGQDMGGL"
     gene            complement(6054..6127)
                     /locus_tag="PKN55_05140"
     tRNA            complement(6054..6127)
                     /locus_tag="PKN55_05140"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6093..6095),aa:Cys,seq:gca)
     gene            complement(6172..6247)
                     /locus_tag="PKN55_05145"
     tRNA            complement(6172..6247)
                     /locus_tag="PKN55_05145"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6212..6214),aa:Gly,seq:gcc)
     gene            complement(6284..6359)
                     /locus_tag="PKN55_05150"
     tRNA            complement(6284..6359)
                     /locus_tag="PKN55_05150"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6324..6326),aa:Gly,seq:gcc)
     gene            complement(6436..7017)
                     /gene="pgsA"
                     /locus_tag="PKN55_05155"
     CDS             complement(6436..7017)
                     /gene="pgsA"
                     /locus_tag="PKN55_05155"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HNX70661.1"
                     /translation="MPFNVPILLTWLRVALIPVFVVLFYWPDPTLHLPVRNGLAALIF
                     ALAAITDWLDGYLARKWGQTSSFGAFLDPVADKLIVAAALILLVELERTQAWLAMVII
                     GREITISALREWMAQIGERRSVAVAYIGKLKTAAQMLAILLLLYWQPLWPGFDTPLYG
                     NILMVIAAILTLWSMVYYLQMAGAQLRGEKKLQ"
     gene            complement(7210..9006)
                     /gene="uvrC"
                     /locus_tag="PKN55_05160"
     CDS             complement(7210..9006)
                     /gene="uvrC"
                     /locus_tag="PKN55_05160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011134859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HNX70662.1"
                     /translation="MFDPKPILDTLPNLPGVYRMLDADAQVLYVGKAIDLKKRVSSYF
                     QKTDLSPRIQLMVRQIAAIETTVVRSEAEALILENNLIKALAPKYNILFRDDKSYPYL
                     MLSGHDWPQLAYYRGALNRHNQYFGPYPNGYAVRESIQVLQRVFRLRTCEDSVFANRS
                     RPCLLHQIKRCSAPCVGLIDAASYQADVRRAAAFLLGKHSELIDDLSAQMQAAAEALA
                     FEQAATLRDQIQALARVQERQFVSSNASQLDCDVIAAVVAQGLACVNLVMIRGGRHLG
                     DKSHFPEHAADMDAGAVLEAFLAQHYVGAPLPPVILVNQPVNEALVAVLQAQAEQRLT
                     VVQHTVGERRVWLEMSEKNARLAIDQRALSKATQHQRLSALAEALALPGVERMECFDI
                     SHTLGEATVASCVVYDHGAMQSSQYRRYNISGITPGDDYAAMRDALTRRYSKLADGEG
                     ELPDALLIDGGKGQVGVAVAVLDELGLSSLPIIGVAKGEGRKPGLETLILPREQKTLQ
                     LPKDHPALHLIQTLRDEAHRFAITGHRARRAKARTQSRLEDIPGVGPKRRQRLLTRFG
                     GLKGVMAASVDELAQVEGVSLALAEKIHQALH"
     gene            9135..10595
                     /gene="guaB"
                     /locus_tag="PKN55_05165"
     CDS             9135..10595
                     /gene="guaB"
                     /locus_tag="PKN55_05165"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012698073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HNX70663.1"
                     /translation="MRIVQKAYTFDDVLLVPAHSSILPRDVSLATQLTRNIRLNLPLV
                     SAAMDTVTESRLAIALAQEGGIGIIHKNMSPARQAAEVSKVKRHESGIVKDPITIKPD
                     MTVGEVIRLVREKKVSGLPVIENGKVVGIVTNRDLRFESRLDAPVRSIMTPRERLVTV
                     KEGASLDEARELMHANRLERVLVINDAFELKGLMTVKDIVKTSEKPFANKDEQGRLRA
                     GAAVGVGEGTEERVALLVEAGVDVIVVDTAHGHSQGVLDRVRWVKQNYPQVDVIGGNI
                     ATAAAALALVEAGADGVKVGIGPGSICTTRIVAGVGVPQLTAVHNVTEALKGTGVPMI
                     ADGGIRFSGDIAKAIAAGANSVMLGGLLAGTEEAPGEIELYQGRSFKSYRGMGSLGAM
                     QQGSSDRYFQDNEANADKLVPEGIEGRVPYKGSAIAVIHQLMGGLRSSMGYCGCATID
                     KMRETAEFVEITSAGMRESHVHDVQITKEAPNYHVD"
BASE COUNT         2007 a         3503 c         3421 g         1769 t
ORIGIN      
        1 gccgggtatt tgtgctgggg ttggcgcaac attgagggtt ctcctgtggt ggtgggcaat
       61 gcgcgaccct cccggcgggc ctgatgggcg cgcaggatgg ggcgtgttgt gtgggttgct
      121 cagtgcatat gcagcgcctg ggcgcgcgcc agcgcttgcg ccggcacact cagccgcacc
      181 gggtagcgcg cggcgtgaat gtccagcgtg gcggccagcg ccggcagcgg cgcggcgtgt
      241 tcgccagaca gcgcgccatc cagctggcgc gcgccagcgc tgagcgcggc caaggcattg
      301 gccaacgcca ggccgtggcg attgtggcaa cgcgcgccta ggcacacttc gctgcccagc
      361 cgggcgcgca ggcggcgcag ggtttccgcc cattgctcgg gcacccagtg caagccggcg
      421 tcgtccagcg tcaggctgtc ggcgcccagc gccgtcgcct ggcgcaaggc gtgcagccag
      481 tcggcgggcc actcggcgcg caccggcagg cgcaaggcgg cgcgcaggcc cagtgcgtcc
      541 agcaggcgca ggcagcggcg cagttcgtcg gcgtcgggcg ggtgggcgtc cagacacagg
      601 cgcacgccgc cttgccgccg gccggcaaac gcctgcgccg ccagccacac cgccatttct
      661 tgcgcggggg cttccagcca gggcggggta tggcagtgcg ccagcagggc gcgcggcgcg
      721 cccagcgcat cgcccagaca caccccggcc aggcccaacg catccagcgc cgccagccaa
      781 gacgcgtgca cgccggccgg cgcctgggcc aggctgacgt ccagcacccg caaggccgtc
      841 accggcgccg ggctggcggc ctggccgcgt tggtagtcgc tcagctcaaa cccccgcccc
      901 aggcgctgcg ccagcacgcg caggcgctcg gcctgcgcca gcggcaggct gccgcgctcg
      961 cgccatttgt aaatcgcccc gcgcgacacc ggctcagccg gaaaatgttc ggccagcagg
     1021 gtggccaggc gggccacgcc gccaaagtcg gcgatcagtt gttcgatggc aagcggcatg
     1081 agggttggct tttggtcaaa atgtctaatt tttacgtgaa taagcgaggt gaaatccaga
     1141 tttactggat attcacaagc agattacggt taactagaca cttaggcaat catccggtac
     1201 gcataaatcc gcaaaaatag acatagtgtc cacaattaag cgacgggccg tctgatgcta
     1261 ccgcacagca aagccggcag aaagcagcac gggccttgca ttgtgcaagg cccgtgctgg
     1321 ggatgatcgc gacgacgaat tccgcccgcg ctacaaccca cccatatcct gtccgccact
     1381 gcggcttgcg accacccgac gcagggcatc caattgcgcc agcatctggt cgcgcagggc
     1441 gtacagctcg gggatgccag tgcgcacgcg cgccagctcg ccggccaggt gggcggcgtg
     1501 cagctggctg gccagttcgt gcacctgatg gtggctggcg tcgatcaccc gaaaggcggg
     1561 cagctggcca tagcggtcct ggcctggccc gtgataccag cggccaaagc ggcagtggtg
     1621 aatgtccagc ggcggcgggc agtggctggc gtcttgctcc aggcattgca ccagctcttc
     1681 aatccagtgc tgatggtcga tgctggcgcc cagcagcgga aaatcatcgc gcgaccaggg
     1741 gtgcaagccg gcgcgccgcc aggattccgg cgcgctaaac ccggccaccc agcctggcac
     1801 ggcctcggcc ggcattggcc gggcaatgcc gtagccctgg cccaggtcgc aacccagctg
     1861 gatcaacagc gcgccgtgct ccagggtttc caccccttcg gcaatcgccg tgcggcggaa
     1921 ggcctgagtc agcccgatca cgccttcgat aatcgccagg tcttccgggt ctttgagcat
     1981 gtcgcggaca aagctctggt cgattttcag cacattggcc ggcaggcgct tgaggtaggt
     2041 cagcgaggaa tagccggtgc caaagtcgtc cagcgcaaac atcaccccca gcgcgcgaca
     2101 gcgctccatc agcgcagaga cataggcaat gtcgtccagc gcggcggttt ccagcacttc
     2161 cagctccagc tggccgggcg gggtgtcggg gtggcgcgcc agcagggtgt gcaggcgctg
     2221 gctgaattgc gggtgttgca ggtggcgggc ggagatattc acgctcaccg gcagggtcag
     2281 cccttgtgcg cgccagacgg ccatctggcg cagggcttct tccatcaccc attcgcccag
     2341 ctccaccgag aattcgctgt cttcgatgat gggcaaaaac tcgcccggcg gcagcaggcc
     2401 gcgctcgggg tgctgccagc ggatcaaggc ttcggcgccc actaccacgc cgtggcgcat
     2461 attcaccttg ggctggtaat acagcctgaa ttcgccagcg ttcagcgccg cctcgatgcg
     2521 cgccaccgcc tcgcggtggg cgtgcgcctg gcggtcgtgt tcggggtcga acaggtgata
     2581 acgatggcgg ccggcctgtt tggccagata catggcctgg tcggcgtggc gcaagagagt
     2641 gtcggcgtcg gcgccgtcca ccggatagag ggtgacgccg atgctggccg acagattggc
     2701 cagccgctcc ttgcccagtt gatacggctg ggcaatggtg tgcagcacgc ggtccagcgc
     2761 ctgctggcat ttgccgatat cgtccagccc cgagagcaag agcacaaact catcgccgcc
     2821 cagccgcgcc acggtgtcgc cattgcgcag cacctgcttg agccggctgg cgacttccac
     2881 cagcagggcg tcgccggctt cgtggccaaa ggtgtcgttc accggcttaa agccgtccag
     2941 atcgagataa cacaccgcca gcagcttgcc cgagcggcgc gcctgggtca gcgccagcat
     3001 cagccggtcg gccagcaaca cccggttggg cagttgggta agcgcgtcgt aatgcgccat
     3061 gcgctcgagt ttttgctggt gttctttcag cacggtgatg tcggagaaca ccgccacata
     3121 gtgcgtcacc cggccgtcgg gcgggcgcac gctggagatg gtcagcagct cggggtacac
     3181 ctcgccgttt ttgcgccggt tccacacttc gccgcgccaa tagccttcgg tgtacaaagc
     3241 cgcccacatt tcactgtaaa acgcttggct gtggtagccc gagcgcagca gattgggcga
     3301 gcgacccagc acgtcttcgc gggcatagcc ggtgatttcg gtaaaggtgc ggttgacgtc
     3361 cacaatctgg gcgttggcgt cggtgatcat gatgccttcg tgggcatgct cgaacacgct
     3421 ggcggtaaag cgcagttgtt cttcggcgcg cttgcgttcg ctgatgtcgt gaatgatgga
     3481 gaacaacagc tcgcgcccgc ccaggcgaat cgggccggaa tagacttcca catcgcgggt
     3541 gctgccgtcg gccaggcggt gaacaaattc aaaacgctgt tggcccgtgg cttgggcgca
     3601 cgccagcgcg gcgcggatgt gttctggggt ggcggtgttg atctggctga tcggcatgcc
     3661 caccagcacc tcgtgcgggt agccataaaa cgcgctggcc gccgggttgg cgtccaccac
     3721 ctgcttgcgc gccggatcga ccagcagctt gacggcatta ttggtttcga acatcgcctg
     3781 ataaaaggcg ctgccttctt ccagcttgag catggccgct ttgcgttcgc tgatatcggc
     3841 cacatagccg taccacagca cgctgccgtc ctccagcgct tccggcatgg cgtgggcttc
     3901 gcgccattcg ggctggccag gctgcaattc cacacggaat tcatcgcgcc atggcgcttg
     3961 ctggcgcgcc gcctgatgca ggccgcgata cagacgcagg cggtcatccg ggtgcagcac
     4021 ccggcatagc gggctggcgt cttcgcgcac gtcttccggg ctgaggccaa atacttcacg
     4081 aatcgcctcg ctggcataag gcagactcag ccgcttgccg tccatatgca tctgacagat
     4141 caggcctggc gcctgttcgg ataatttatg cagcagcttg tcgcgcgcgc tcaagcgctc
     4201 ctgggcaaga atttgcgcgc tgatgtcggt ttgcacacca atatagtggg tgagctgctg
     4261 acgctcgtca tacaccggcg caatgctcag ctcgttccag aacagcgagc cgtccttgcg
     4321 gtaattgcgc agcacggtct ggcaggattt acccgcggcc atggccgtgc gcaaggcgtc
     4381 cagccctggc tgctcgcggt cgccctggtt gagcatgcgg caattgcgcc ccagcacttc
     4441 ttcgcgccgg tagccggtag tgcgctcgaa ggcgtcgttg acgtagataa tcggctgatc
     4501 gggctggcgc acgtcgctga tgatgacgcc attgcgcgcc gaatgcagcg cccgctcgag
     4561 aagcttgagc cagtacaggg tgtctttgta gcgggtgatg tcggtgaggg tgccaatcac
     4621 ccgcgacggc gcgccgtcgc ggtcgcggct gaccacctgg ctgcgcaggc cataccagcg
     4681 ccactggcca ttgccggcgc gcaggcggat ttcgctgccg tgcgggcgca gttcgtggct
     4741 gctggccggc tgctcacgtc cggccagtac ggcaatgaaa gcaggcaagt cgtccggatg
     4801 gacacgggtt tcccagccgg taagtttatt gcccagctcg gcggcgctca cccccagcag
     4861 catgcgccag cgcggcgaat actggatgcg ctggctgcgc gcgtgccaat cccacacccc
     4921 ggtgtcggcg ccttccaggg caaactgcca gcgccgttca ttggcggaca gttccgccac
     4981 cagcgcgctg agcagcagcg ccaccaccat cagcgtggcc atatagcccc acagcagcgc
     5041 caggccttcg tgcaccgagg ggcgatgaaa cggccccatg ccctgcaccg tgccccaggc
     5101 catcagcgcc gacaacagca gcaccgccag cgaggacagc atcagcccgg tgcgcaccgc
     5161 cagccaggac aacagcagga acggaataaa caccaagggc aacagcgccg cgtgcgaggg
     5221 ggaatacagc agcaactggc caatcatcag catgccagcc aggcagccca gcccttccaa
     5281 tcggcgggta ggtgataatt cgcgccacaa ccggggcaca aaggtcagca gcggaatgcc
     5341 cagcagcaaa gcccccaccg catcgccagc ccaccagatc aaaaaaatct tgggcaattg
     5401 cgccggctgc atcaccccgg acagccacag actgagtgtg cccaccgagg ccgaggccag
     5461 catgccgccg ctgatggcca gcaggtagtg gttcatgtcg cggcgggtgt caaagtgcag
     5521 gctggcgccc acccggcgca aggcccaaac ggtgagcaag ggggccaggg tattgccgca
     5581 ggcaatcgcc accgacatca gcggtgtgcc gcccgaggcc aggttgatag ccagcgagcc
     5641 cagccagacg ccaggccagc gcgacagccc accgcgccac agcgccgcca ccgccacgcc
     5701 cgtgggcggc cagatcagcg acacaaacgc gcccagataa ggcacggtca ggcccagctg
     5761 gccgctggca aaatacgcca gcgccaggcc cagcaaccaa agccaggtgt gcagcacgcc
     5821 cttcatgcgt ttgccaccct ctggggcagc ttatgtcttg ccggatggaa aaccagcatc
     5881 gtcagccact cgttaacaac aatcaggccg agagactacc attgtcgggc gatagcgggt
     5941 agccgcccaa gggcgtgggc gacgggtttt gcgtgggatt ttggcatgga tcaagagacg
     6001 gcgcgccatg caaaaagccc tgaaccagtg tccagggctt gatgagcgct ttttggaggc
     6061 ggaggccgga atcgaaccgg cgtggacggc tttgcaggcc gctgcataac cactttgcta
     6121 ccccgccgaa ggcgttgcaa ttcgcagtca ggctgcgcct tgcgcgaaaa ctggagcggg
     6181 aaacgaggct cgaactcgcg acctcaacct tggcaaggtt gcgctctacc aactgagcta
     6241 ttcccgcatc gccaaacttg tcgcatgctg aaacataaga aactggagcg ggaaacgagg
     6301 ctcgaactcg cgacctcaac cttggcaagg ttgcgctcta ccaactgagc tattcccgcg
     6361 ctgttgtttc ttgcgttaca gcaatcgctg caagagactg cgcattataa gcaggttttt
     6421 ttttgtgtca acacttcatt gcaatttttt ttcgccgcgc aactgcgcgc cagccatttg
     6481 caggtaataa accattgacc ataaagtcaa aatcgcggca atcaccatca ggatgtttcc
     6541 gtacagcggc gtatcaaaac ccggccacaa gggctgccag tacaacagca gcaaaatggc
     6601 cagcatttgc gcggcggttt tgagcttgcc gatataggcc accgccacac tgcggcgctc
     6661 gccaatttgc gccatccact cgcgcaatgc cgaaatggtg atctcgcggc cgatgatcac
     6721 catcgccagc caggcctggg tgcgctccag ctccaccagc aaaatcagcg cggcggcgac
     6781 aatcagcttg tcggccaccg ggtcaagaaa agcgccaaaa gaagaggttt gcccccactt
     6841 gcgcgccaga taaccatcca gccagtcggt gatggccgcc agggcaaaga tcagcgccgc
     6901 caggccattg cgcacgggta gatgcaaagt ggggtctggc caataaaaca gcacgacaaa
     6961 caccgggata agggccaccc gcaaccaggt gagcagaatg ggaacattga acggcatggg
     7021 cgatctggcg cttttccgcc ggcgggcggc gcgtcggcaa taacagtttc ggggaacagc
     7081 cgtcagtgta gcgcgacggc cggcgcgcca gtgcgagcaa gggcgcgggg tcacacaaga
     7141 ttcatccggg cgggcagcga ggcgcgctgc gctgcgtgtt gtgagccgct ctgagtcgct
     7201 gagcggcgct cagtgcagcg cctggtggat tttttccgcc agcgccaggc tgacgccttc
     7261 cacctgggcc aactcgtcca cactggcggc catcacccct ttcagcccgc caaaacgggt
     7321 gagcaggcgt tggcggcgtt tgggccccac gccggggatg tcctccagcc gcgactgcgt
     7381 gcgcgccttg gcgcggcggg cgcggtggcc ggtgatggca aagcggtgcg cctcgtcgcg
     7441 caaggtttga atcaagtgca gcgccggatg atcttttggc aattgtaacg ttttttgttc
     7501 gcgcgggaga atcagcgttt ccagccccgg cttgcgcccc tcgcccttgg ccacgccgat
     7561 gatgggcagc gacgacaaac ccagctcgtc cagcaccgcc accgccacgc ccacctggcc
     7621 cttgccgccg tcgatcagca gcgcatccgg cagctcgcct tcgccgtcgg ccagcttgct
     7681 gtagcggcgg gtgagcgcgt cgcgcatcgc cgcgtaatca tcgcctgggg taatgccgct
     7741 gatgttgtag cgccggtact ggctggactg catggcgccg tggtcgtaca ccacgcagga
     7801 cgccaccgtc gcctcgccca gggtgtggct gatgtcaaaa cactccatgc gctcgacgcc
     7861 gggcagcgcc agcgcctcgg ccagcgcgct cagccgctgg tgctgggtgg ccttggacaa
     7921 ggcgcgctga tcaatggcca gcctggcgtt tttttctgac atctccagcc acacccgccg
     7981 ctcgcccacc gtgtgctgca ccaccgtcag gcgctgctcg gcctgcgcct gcaacaccgc
     8041 caccaaggcc tcgttgaccg gctggttgac caaaatcacc ggcggcagcg gcgcgcccac
     8101 gtaatgctgg gccaaaaacg cctccagcac cgcgccagcg tccatatcgg cggcatgctc
     8161 aggaaaatgg ctcttgtcgc ccagatgacg gccgccgcga atcatcacca gattgacaca
     8221 ggccagccct tgcgccacca cggcggcaat cacgtcgcaa tccagctgcg aggcgttgct
     8281 gctgacaaac tggcgctctt gcacccgcgc cagcgcctga atctggtcgc gcaaggtggc
     8341 cgcctgctcg aacgccagcg cctcggccgc cgcctgcatc tgcgccgaca aatcatcaat
     8401 cagctcggaa tgcttgccca gcaaaaacgc cgccgcgcgg cgcacatcgg cctgatagct
     8461 ggcggcgtcg atcagcccga cgcacggcgc tgaacagcgc ttgatctggt gcagcaaaca
     8521 cgggcgcgag cggttggcga atacgctgtc ttcgcaggtg cgcaggcgaa acacccgctg
     8581 caacacctga atcgactcgc gcaccgcgta gccattcggg taggggccaa aatactggtt
     8641 gtgccggttc agcgcccccc ggtaatacgc cagctgcggc cagtcgtgac cggacagcat
     8701 caggtaggga taggatttat cgtcgcggaa cagaatattg tatttggggg ccagcgcctt
     8761 gatcaggttg ttttccagaa tcagcgcctc ggcctcagag cgcaccaccg tggtttcgat
     8821 ggcggcaatc tggcgcacca tcagctggat gcgcgggctg aggtcggttt tctggaaata
     8881 cgaactgacc cgttttttca ggtcgatggc cttgccgacg tacaacacct gcgcgtcagc
     8941 gtccagcatg cggtaaacac ccggcagatt gggcagggtg tccagaatcg gtttggggtc
     9001 gaacatgggc gggcaatccg gcgggggtgg gcggcaatgc ccacagcggt taaccgggta
     9061 ttgtgcgccc ttgaccaaat tttgccaagc aaggattttt cggtataata agcctttgcc
     9121 gcaaaggaat tcccatgcgc atcgtccaga aagcctacac cttcgacgac gtcctgctcg
     9181 tccctgccca ttccagcatc ctgccgcgcg acgtgagtct ggccacccag ctgacccgca
     9241 acatccgcct gaacctgccg ctggtgtccg ccgccatgga caccgtcacc gaaagccgcc
     9301 tggccatcgc cctggcgcag gaaggcggca tcggcatcat ccacaagaac atgtcgcccg
     9361 cccgtcaagc ggcggaagtc tccaaggtca agcgccacga gtccggcatc gtcaaggatc
     9421 cgatcaccat caagccggac atgaccgtcg gcgaggtcat ccgcctggtg cgcgagaaaa
     9481 aagtctccgg cctgccggtg atcgaaaacg gcaaggtggt gggcattgtc accaaccgcg
     9541 acctgcgctt tgaatcgcgc ctggacgccc cggtgcgcag catcatgacc ccgcgcgagc
     9601 gcctggtcac cgtcaaggaa ggcgcctcgc tggacgaagc gcgcgagctg atgcacgcca
     9661 accgcctgga gcgcgtgctg gtgatcaacg acgcctttga actcaagggc ctgatgaccg
     9721 tcaaggacat cgtcaagacc agcgaaaaac cgtttgccaa caaagacgag caaggccgcc
     9781 tgcgcgcggg cgccgccgtc ggcgtgggcg aaggcaccga agaacgcgtg gccctgctgg
     9841 tggaagccgg cgtggacgtg atcgtggtgg acaccgccca cggccacagc cagggcgtgc
     9901 tggaccgcgt gcgctgggtg aagcagaact acccgcaagt ggacgtgatc ggcggcaata
     9961 tcgccaccgc cgccgccgcg ctggcgctgg tggaagccgg cgctgacggc gtgaaagtgg
    10021 gcatcggccc cggctcgatc tgcaccaccc gcatcgtcgc cggcgtgggc gtgccgcagc
    10081 tgaccgccgt gcacaatgtc accgaagcgc tgaagggcac gggcgtgccg atgatcgccg
    10141 acggcggcat ccgcttctct ggcgacatcg ccaaagccat cgccgctggc gccaacagcg
    10201 tgatgctggg cggcctgctg gccggcaccg aagaagcgcc gggcgaaatc gagctctacc
    10261 aaggccgctc gttcaagagc taccgtggca tgggctcgct gggcgccatg cagcaaggct
    10321 ccagcgaccg ttacttccag gacaacgaag ccaacgccga caagctggtg ccggaaggca
    10381 tcgaaggccg cgtgccctac aagggctcgg ccatcgccgt catccaccaa ctgatgggcg
    10441 gcctgcgctc gtccatgggc tactgcggct gcgccaccat cgacaagatg cgcgaaaccg
    10501 ccgagtttgt ggaaatcacc tccgccggca tgcgcgaatc gcatgtgcac gacgtgcaaa
    10561 tcaccaaaga agcgccgaac taccacgttg attgatgacg tgcgcggcct ggtgaggccc
    10621 gactggacaa aaaaacccgt cgccagaatt ggcggcgggt tttttgatgg gcggcgcgaa
    10681 cagcaagcgc agttggcgcg
//
LOCUS       DAOPYX010000069        10667 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_7155_length_10667_cov_8.898511, whole genome shotgun sequence.
ACCESSION   DAOPYX010000069 DAOPYX010000000
VERSION     DAOPYX010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 10667)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10667)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10667
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7155_length_10667_cov_8.898511"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1954)
                     /locus_tag="PKN55_05170"
     CDS             complement(<1..1954)
                     /locus_tag="PKN55_05170"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008953245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HNX70664.1"
                     /translation="MTARTPLHGLQVANELHRFVEEQVLPGTGVNSEAFWAGFSQIVS
                     DLAPKNAALLAERDRIQAEMDAWHQAHPGPIADMAAYQAFLAQIGYIVPAPADVHATT
                     SNVDDELAVQAGPQLVVPVLNARYALNAANARWGSLYDALYGTDAIPEDGGAEKGAGY
                     NPARGAKVIAFARQFLDQSAPLAGASHQDAAGYAIVDGQLAVKLANGGSAALAKPAQL
                     VGYQGDAAAPSSVLLVNNGLHIDIRIDRSTTIGASDAAGVADVVLEAALSTILDLEDS
                     VAAVDAEDKVLGYSNWLGILRGTLTETFSKGGKALTRGLNQDRVYTAANGQGEVTLHG
                     RSLLFVRNVGHLMTNPAILWGAEGKEIPEGIMDAVITTTIAIHDLKGLGQSGLKNSRK
                     GSVYIVKPKMHGPAEVAFACELFSRVEQLLGLPEATVKLGIMDEERRTSVNLKACIAA
                     ASSRVAFINTGFLDRTGDEMHTAMLAGPMMRKGDIKTSAWIAAYERRNVLIGLQCGLR
                     GRAQIGKGMWAMPDLMAEMLKQKIGHPKAGANTAWTPSPTAATLHALHYHQVLVADVQ
                     KAIEASGDADNADVTAKLLADLLTIPVVAEAQWSEAEKQQEIDNNIQGILGYVVRWVD
                     QGVGCSKVPDINDIGLMEDRATLRISS"
     gene            2649..3044
                     /locus_tag="PKN55_05175"
     CDS             2649..3044
                     /locus_tag="PKN55_05175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX70665.1"
                     /translation="MDYPAVASLNVALGNEAALHALRQQQTCYTRVGDSMLRVMREAA
                     REKPWQRKRERLACIGFSDSLALSHDLSVQALQAQVSQWQLMYDACGQPAWAKPWGDY
                     LQQEIRNTSRFFTALGRASGGLAYAGLVN"
     gene            complement(3168..5006)
                     /locus_tag="PKN55_05180"
     CDS             complement(3168..5006)
                     /locus_tag="PKN55_05180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008953089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HNX70666.1"
                     /translation="MKRLAPLTLALSLAYSAAQAGTFSSYDDASLQVGQQLMQSNGLL
                     LRNGDDVWKRIREGYQLTEVDSELVRRHERMYSAKPEYLKRTLTRGQRYLFHIMNEVE
                     RRGMPTEIALLPLVESAFNPTAVSGAKAAGLWQFMPATGRHYGLEQTFWYDGRRDVLG
                     ATNAALDYLENLYAMFGDWNLALASYNWGEGNVSRALERNRAKGLPETFEAIRMPNET
                     RNYVPKLLAVRNILAEPERFGLKLDKFDNRPYFVAVSTGKHMDIDVAAKLAGMSLDEF
                     KALNPAFNRPIYAHKPGRQLLVPASKAELFEKNLAEYREPLLSWQVYTAQSGDNIDEL
                     AQRHGMSVERLRNVNGLASNSLAPGQPLLLAALSSQTASPAPAALNTDVDTTPDTYVS
                     PLLTANAGAGAAVERLAQADKPATADSKPAVVRVSADSEAKPRPATVASEPARAVQTA
                     AADANGGSTRRVSAVLTPSPAVASNTVASSADTPATSRHTVAPGDTLYNISRRYNLSV
                     AELKTLNQLDGEAVKLGQTLAVKLMATAVAAAAPSASPAKADKADKPEHKEVRKEYVV
                     QRGDTLFSIARKFNIDHDDLKKWNPTHALARLQPGFKLTLWTSSLR"
     gene            complement(5154..5915)
                     /gene="gloB"
                     /locus_tag="PKN55_05185"
     CDS             complement(5154..5915)
                     /gene="gloB"
                     /locus_tag="PKN55_05185"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018749219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HNX70667.1"
                     /translation="MLRVSPVPAFADNYIWVLHDSTHALVVDPGDAAPVQAFLAEHQL
                     TLAGVLITHGHIDHIGGVSALRADWPETPVYGPADVACVTQPVGEGESASLPGHGEAL
                     TVWATPGHTANHLSYLLPGAVFCGDTLFACGCGRVFDGTPTQLHASLQRLAALPDDTQ
                     VYCTHEYTLANQRFARHLEPDNAVLLARCAADEALRAQGLPTVPTTIGLERQTNPFLR
                     VNQPAVRAALAAELGEAPADALAAFTALREWKNRF"
     gene            5979..6737
                     /locus_tag="PKN55_05190"
     CDS             5979..6737
                     /locus_tag="PKN55_05190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011134809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNX70668.1"
                     /translation="MPALNSWLDTPLGQHVLAREQAYFDQAVADVFGYHAVQFGLPDQ
                     PLLRENRMPWRCRAGESGAVDVTCDPAALPFASQSLDLLLLPHVLDFTSHPHQVLREA
                     ERVLVPEGRLMVTGFNPISLWGLARRCKPRDVMPWRANFIAPGRLKDWMQLLSLEPAD
                     CQHLCYAPPFEREAWRARWGFMERLGARWWPLAGGVYAISAVKRVRCMRLLAPRWSLL
                     TPAPLAGVAARPAGERGAVSPSLNPKAPSMKQTE"
     gene            6734..7183
                     /gene="rnhA"
                     /locus_tag="PKN55_05195"
     CDS             6734..7183
                     /gene="rnhA"
                     /locus_tag="PKN55_05195"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003773666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HNX70669.1"
                     /translation="MSTEFVEIYTDGACKGNPGPGGWGALLRYKGQEKELWGGELDTT
                     NNRMELLAVIQALNSLKRPCAIHLYTDSQYVQKGISEWIHGWKARGWKTAAKEPVKNA
                     DLWRLLDAATGQHQIDWRWVKGHAGHEFNERADALANRGVETALAGR"
     gene            7201..7914
                     /gene="dnaQ"
                     /locus_tag="PKN55_05200"
     CDS             7201..7914
                     /gene="dnaQ"
                     /locus_tag="PKN55_05200"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011134811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HNX70670.1"
                     /translation="MRYIILDTETTGLNPRTGDRLVEFAGLEMVGRKLTGRSLHLYVH
                     PERDIPEEASRIHGITLDQLEGKPTFAGVAAEISEFIRGAALIIHNAPFDMGFLDMEF
                     TRLGLPSAREQVGEVIDTLAMAKDQFPGKRNNLDALCDRFEVDRSNRVFHGALIDCEL
                     LGEVYLAMTRGQESLLMDIEPLSSERGGPSMRLADGPRPRVQAASAEELAAHEGYLDE
                     LDKAMKGPCLWRQTPPEAA"
     gene            7911..8612
                     /gene="ispD"
                     /locus_tag="PKN55_05205"
     CDS             7911..8612
                     /gene="ispD"
                     /locus_tag="PKN55_05205"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012696367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HNX70671.1"
                     /translation="MSARRLAVVPAAGSGSRFGQATPKQYTLLAGQPLLAHTLAVLCA
                     EPRIDQVWVVISPDDDWFEPFGFAQQAWAQSKLRVLRQGGATRAESVRNGLAALDAQP
                     DDWVLVHDAARCCLPAEALARLIDELDADPCGGLLALPVADTLKRQDKDSRVAATVPR
                     DGLWQAQTPQMFRHGLLSQALAISSSADITDEASAIERLGLCPRLVRGDASNFKVTYA
                     QDIRLAAALLSAGQN"
     gene            8637..9113
                     /gene="ispF"
                     /locus_tag="PKN55_05210"
     CDS             8637..9113
                     /gene="ispF"
                     /locus_tag="PKN55_05210"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014086347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HNX70672.1"
                     /translation="MIRIGQGWDIHKLVEGRPLILGGVTIPHDKGLLGHSDADALLHA
                     ITDALLGAAGLGDIGSHFPDTDPAFHGVDSRILLRETAARLRERGWKVVNVDSTVIAQ
                     RPKLAGHIAAMRANIATDLRLKMDEVNVKAKTSEKLGPMGREEGIAAQAVCLIERA"
     gene            9171..9836
                     /gene="rpiA"
                     /locus_tag="PKN55_05215"
     CDS             9171..9836
                     /gene="rpiA"
                     /locus_tag="PKN55_05215"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011134814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HNX70673.1"
                     /translation="MSTLTQDDKKRAVARKAIDFVPDDSIIGVGTGSTVNFFIDELAR
                     IKARIDGCVSSSDASTARLKAHGIPVYELSSVGELQVYIDGADEVNHSLHMIKGGGGA
                     LTREKIVASAAREFICIADEAKYVSKLGAFPVAVEVIPMARSVVARELVHLGGHPELR
                     QGFTTDNGNLILDVHDLDILKPLEMETAINQIAGVVCCGIFARQHAHVLLLGADDGVK
                     VIR"
     gene            9984..>10667
                     /gene="phoU"
                     /locus_tag="PKN55_05220"
     CDS             9984..>10667
                     /gene="phoU"
                     /locus_tag="PKN55_05220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014086348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HNX70674.1"
                     /translation="MAEHISKQFDLELETIRTRVLQMGGLVEAQIVGAIDGLMSSDIA
                     KLDKVIAEDALVNAMEVSLDEECQHIIARRQPAASDLRMVITVIKTITDLERIGDEAQ
                     KVARMGKTILLADRVHMPRFREIQKMAEVALAMLRRALDAFARLDVAGALDVARQDRK
                     LDEEFDANFRQLVTFMMEDPRAISMSIDTIFMCKAIERIGDHAKNISEYVVYLVKGKD
                     IRHTALEDVE"
BASE COUNT         1833 a         3290 c         3565 g         1979 t
ORIGIN      
        1 ggctggagat gcgcagggtg gcgcggtctt ccatcaggcc gatgtcgttg atgtccggca
       61 ccttggagca gcccacgcct tgatcgaccc agcgcaccac gtaacccaga atgccctgga
      121 tgttgttgtc gatttcttgc tgcttttcag cttcgctcca ctgggcttcg gccaccaccg
      181 ggatggtcag caggtcggcc agcagtttgg cggtgacgtc ggcgttgtcg gcgtcgccgc
      241 tggcttcgat ggctttttgc acgtcagcca ccagcacttg atggtagtgc agggcgtgca
      301 gggtggcggc ggtggggctg ggggtccagg cggtgttggc gccggctttc gggtggccga
      361 ttttctgctt gagcatttcg gccatcaggt cgggcatggc ccacatgccc ttgccgatct
      421 gggcgcggcc gcgcaggccg cattgcaggc cgatcagcac attgcggcgc tcgtaggcgg
      481 caatccaggc gctggttttg atgtcgccct tgcgcatcat cgggccggcc agcatggcgg
      541 tgtgcatttc gtcgccagtg cggtccaaaa agccggtgtt gatgaaagcc acgcgcgacg
      601 aggcggcggc gatgcaggcc ttcaggttga ccgaggtgcg gcgttcttcg tccatgatgc
      661 ccagcttgac ggtggcttcc ggcaggccca gcagttgttc gacgcggctg aacagttcgc
      721 aggcaaaggc cacttcggcc gggccgtgca tcttgggctt gacgatgtac accgagccct
      781 tgcgcgagtt tttcaggccg gattggccca ggcccttgag gtcgtggatg gcgatggtgg
      841 tggtgatcac cgcgtccatg atgccttccg ggatttcctt gccttctgcg ccccacagga
      901 tggccgggtt ggtcatcagg tggccgacgt tgcgcacaaa cagcaggctg cggccgtgca
      961 gggtgacttc gccctggccg ttggcggcgg tgtacacgcg gtcctggttc aggccacggg
     1021 tcagcgcctt gccgcccttg ctgaaagttt cggtcagcgt gccgcgcaga atgcccagcc
     1081 agttgctgta gcccagcact ttgtcttcgg cgtccacggc ggcgaccgag tcttccaggt
     1141 caagaatggt ggacagcgcg gcttccagca ccacgtcggc cacgccggcg gcgtccgagg
     1201 cgccgatggt ggtgctgcgg tcgatgcgga tgtcgatgtg caggccgttg ttcaccagca
     1261 gcacgctgct gggggcagcg gcgtcgcctt ggtagcccac cagttgggcc ggcttggcca
     1321 gcgcggcgct gccgccattg gccagcttga ccgccagttg gccgtccacg atggcgtagc
     1381 cagcggcgtc ctggtggctg gcgccagcca gcggggcgga ttggtcgagg aactgacggg
     1441 cgaatgcaat caccttggcg ccgcgcgccg ggttgtagcc agcgcctttc tcggcgccgc
     1501 cgtcttccgg gatggcgtcg gtgccgtaca gggcgtcgta caggctgccc cagcgggcgt
     1561 tggcggcgtt cagcgcgtaa cgggcgttca gcaccggcac caccagttgc gggccggctt
     1621 gcacggccag ttcgtcatcc acattgctgg tggtggcgtg cacgtcggcc ggcgccggca
     1681 cgatatagcc gatttgcgcc aggaaggctt gataggcggc catgtcggca atcgggccgg
     1741 ggtgggcttg atgccaggcg tccatctcgg cctgaatgcg gtcgcgctcg gccagcaggg
     1801 cggcgttttt cggggccagg tcgctgacaa tctggctaaa gccggcccag aaggcctcgc
     1861 tgttcacgcc agtgccgggc agcacctgtt cttcaacaaa acgatgcagt tcgttggcga
     1921 cttgcagacc gtggaggggg gtgcgtgcgg tcatgaaaca acctttcaaa tcaataagtt
     1981 ttttcgatgt ctgtgtggcg cggcccatcg cgcccggcta tttgggcgtc gccatgctgc
     2041 gcgcattggc gcggcgacgt ccggcgggcg tcgtgggctg ctgtgtgtcc gcgccagggg
     2101 cgcggcggcg caagcgctgt caacggcggg gtgtggcggc tggcggtggg ccagcgcgcg
     2161 ttgagcaggc tgtgcggctg ttgtggctga caaacaggtt ctggccgggc gcagggcttg
     2221 tggcccgctt ccccgcgccg ccgaagcggg cgcggcgctg acgtgcgcgg catgtttgga
     2281 taacgcggta ttgttgcatg aactgtccgc tattccaaat agcgcgtcgc ttttttgtag
     2341 tttgtcggcc tgaagttgac gtgggcggaa agccggccgg gccgggcgat gaccatggtc
     2401 acattgtgga agtgctgtgg ctgctttatg ttgctttgca atatgcatgt ggcggttggt
     2461 gcgctgcgcc tagccattgg catggattct gcttaaccct gatgtcaatg tggaaatatt
     2521 tggtttttaa gtacatttct tttgcgtgta agcgacattg ccgacaaaaa atcggccagg
     2581 ctttttgctg tttgccgtag tttcacgtat agtgatgttg caatgcacaa atttggaaag
     2641 gacgcatcat ggactatcct gccgtagcct ccctcaatgt tgcgctgggc aatgaagccg
     2701 ccttgcatgc cctgcgccag caacaaacct gttatacccg cgtgggtgat tcgatgctgc
     2761 gcgtgatgcg cgaagccgcc cgtgaaaaac cgtggcagcg caagcgcgag cgcctggcct
     2821 gcattggctt cagcgacagc ctggccctga gccatgactt gtcggtgcaa gcgctgcaag
     2881 cacaggtcag ccagtggcag ctgatgtacg atgcctgcgg ccaaccggcc tgggccaaac
     2941 cctggggcga ttatttgcaa caggaaatcc gcaatacctc gcgctttttt accgcgctgg
     3001 gtcgtgccag cggtgggctg gcgtatgcgg gcctggtgaa ttgatcggcg cgtattgcgc
     3061 cgcgtagggc agtcagcgtc gtcaggcgct ggtgtaaaaa aaaccgccgc aggtgccaac
     3121 acctgcggcg gtttttttta cgtcatactt tcttcaccca aaacccatca gcgcaggctt
     3181 gaagtccaca gggtgagctt gaaacccggt tgcagccgcg ccagcgcgtg ggtggggttc
     3241 cattttttca ggtcgtcgtg gtcgatattg aacttgcggg cgatgctgaa caaggtgtca
     3301 cccctttgca ccacgtattc tttgcgcact tccttgtgct cgggcttatc ggctttgtct
     3361 gccttggccg gcgaggccga tggcgcggcg gcggccacgg cggtggccat cagcttcacc
     3421 gccagggttt gccccagctt cacggcttcg ccatccagct ggttcagggt tttcagctca
     3481 gccacgctca gattgtagcg gcgcgagata ttgtacagcg tgtcgcccgg cgccacggtg
     3541 tggcggctgg tggccggggt gtcggcgctg ctggccacgg tattgctggc cacggccggc
     3601 gagggcgtca gcacggcgct gacgcggcgg gtgctgccgc cattggcgtc ggcagcggcg
     3661 gtttgcacgg cgcgggccgg ttcgctggcc actgtggccg ggcgcggttt ggcttcgctg
     3721 tcggcgctga cccgcaccac ggcaggcttg ctgtcagcgg tggcaggctt gtcggcctgg
     3781 gccagacgtt cgacggcggc gccagcgccg gcgttggcgg tcagcagcgg agagacgtaa
     3841 gtgtccggcg tggtgtccac atcagtattc agcgcggccg gcgccgggct ggcggtctgg
     3901 ctgctcagcg cggccagcag cagcggctgg ccgggggcca ggctgttgct ggccaggcca
     3961 ttgacattgc gcaggcgctc cacgctcatg ccgtggcgct gggccagttc gtcgatattg
     4021 tcaccggatt gggcggtgta cacctgccag gacagcaggg gctcgcggta ttcggccaga
     4081 tttttttcga acagctcggc cttgctggcc ggcaccagca actgacggcc aggcttgtgg
     4141 gcgtagatcg ggcggttaaa cgccgggttg agggctttga attcgtccag cgacatgccg
     4201 gccagcttgg cggcgacgtc gatgtccata tgcttgccgg tgctcaccgc cacgaaatac
     4261 gggcgattgt cgaatttgtc cagtttgagg ccaaagcgct cgggttcggc cagaatattg
     4321 cgcacggcca acagcttggg cacgtagttg cgggtttcgt tgggcatgcg gatggcttca
     4381 aacgtctccg gcaggccctt ggcgcgattg cgctccagcg cgcggctgac attgccttcg
     4441 ccccagttgt aggacgccag cgccagattc cagtcgccaa acatggcgta gaggttttcc
     4501 aggtaatcca gcgcggcgtt ggtggcgccc agcacatcgc ggcggccgtc gtaccagaag
     4561 gtttgctcca ggccatagtg gcggccggtg gcgggcatga actgccacag gccggcggcc
     4621 ttggcgccgg ataccgcggt gggattgaag gcgctttcca ccagcggcaa cagggcgatt
     4681 tcggtcggca tgccacggcg ctcgacctcg ttcatgatgt gaaacagata gcgctgacca
     4741 cgggtcaagg tgcgcttgag gtattccggc ttggcgctgt acatgcgctc gtggcggcgc
     4801 accagctcgc tgtccacttc ggtgagctga tagccttcgc ggatgcgctt ccagacgtcg
     4861 tcgccgttgc gcagcagcag gccgttgctt tgcatcagct gctggccaac ttgcagcgag
     4921 gcatcgtcat aagaggagaa tgtccccgcc tgggcagcgg aataagccag tgagagcgcc
     4981 agtgtcagcg gggccagtcg tttcatgcca aaatgacctt tattttcaaa gttgcagcga
     5041 tgctagtttt acttggcgag gctgtcaacc cataaaaatc aatcattttg cgcattgccg
     5101 gcaaaatttg ccgcctgtgc cgcgctggcc tgagttgcgc attccatttg cattcaaaag
     5161 cggtttttcc attcacgcaa ggcggtgaac gccgccagcg catcggcggg cgcttcgccc
     5221 agttccgcag ccagcgcggc gcgcaccgct ggctgattca cccgcaaaaa cggattggtt
     5281 tgccgctcca gaccgatagt ggtcggcacg gtgggcaggc cttgcgcgcg cagggcttcg
     5341 tcggcggcgc agcgcgccag caagacggcg ttgtccggct ccagatggcg ggcaaagcgc
     5401 tggttggcca gggtgtattc gtgcgtgcaa tacacctggg tgtcgtcggg cagggcagcc
     5461 aggcgttgca aggaggcgtg cagctgcgtt ggcgtgccgt caaacacccg gccacagccg
     5521 caggcaaaga gcgtatcgcc gcaaaacacc gcgccgggca gcagatagct gagatgattg
     5581 gcggtgtggc cgggcgtggc ccacaccgtc agcgcttcgc catggccggg caggctggcg
     5641 gattcgccct cgcccaccgg ctgggtgacg caggccacat cggccgggcc atacactggc
     5701 gtctcgggcc agtcggcgcg caatgccgat acgccgccaa tgtgatcaat atggccatgg
     5761 gtgatcaaca cgccggccag cgtcagctga tgttcggcca aaaacgcctg aactggcgcg
     5821 gcgtcgcctg ggtccaccac cagcgcatgc gtcgagtcgt gcaaaaccca gatataattg
     5881 tcagcaaatg ctggaacggg agacacgcgc aacatgaaaa ctccgtttct gtcggacagg
     5941 atcttgatct tggcgcacac aaccccggga gggctggcat gccggcgttg aactcatggc
     6001 tggacacgcc gctgggccag catgtgctgg cgcgcgagca ggcgtatttc gaccaggcgg
     6061 tggccgatgt gtttggctat cacgccgtgc agtttggctt gcccgatcag ccgctgctgc
     6121 gggaaaaccg catgccgtgg cgctgccgcg ctggcgagag cggcgcggtg gacgtcacct
     6181 gcgacccggc ggcgctgccg tttgccagcc agagcctgga tttgctgctg ctgccgcatg
     6241 tgctggattt taccagccat ccgcatcagg tgctgcgcga agccgagcgg gtgctggtgc
     6301 cggaaggccg tctgatggtc accggcttta acccgatcag cctgtggggg ctggcgcggc
     6361 gctgcaaacc gcgcgatgtg atgccctggc gggccaattt tattgcgcca ggccggctga
     6421 aagactggat gcagctgctg agcctggagc ccgccgactg ccagcacctg tgttacgcgc
     6481 cgccgtttga gcgcgaagcc tggcgcgcgc gctggggctt tatggagcgc ctgggcgcgc
     6541 gctggtggcc gctggccggc ggcgtgtacg ccatttcggc ggtcaagcgc gtgcgctgca
     6601 tgcgcctgct ggccccacgc tggagcctgc tgaccccggc gccgctggcc ggggtggcgg
     6661 cgcgcccggc gggcgagcgc ggcgcggtgt ctccctctct gaaccccaaa gccccttcca
     6721 tgaagcagac tgaatgagca ctgaatttgt cgagatttac accgatggcg cctgcaaggg
     6781 caaccctggc ccaggcggct ggggcgcctt gctgcgctac aaaggccagg aaaaagaact
     6841 ctggggcggc gagctggaca ccaccaacaa ccgcatggag ctgctggcgg tgatccaggc
     6901 gctcaacagc ctcaagcgcc cctgcgccat ccacctctac accgactcgc aatatgtgca
     6961 aaaaggcatc agcgagtgga ttcacggctg gaaggcgcgc ggctggaaaa ccgccgccaa
     7021 agagccggtc aaaaacgccg acctgtggcg tcttctggac gccgccaccg gccaacacca
     7081 aatcgactgg cgctgggtca agggccacgc cggtcatgaa ttcaacgaac gggccgacgc
     7141 cctggccaat cgcggcgtgg aaaccgcgct ggccgggcgc tgagccccgg caaggcatgt
     7201 atgcgttaca tcattctcga tactgaaacc accggcctga acccgcgcac cggcgaccgc
     7261 ctggtggaat ttgccgggct ggaaatggtc gggcgcaagc tcaccggccg ctcgctgcac
     7321 ctgtatgtgc accccgagcg cgacatcccg gaagaagcct cgcgcattca cggcatcacc
     7381 ctggatcaac tggaaggcaa gcccaccttt gccggcgtgg cggctgaaat cagcgagttt
     7441 attcgcggcg ccgcgctgat catccacaac gccccgtttg acatgggttt tctggatatg
     7501 gaattcaccc gcctgggcct gcccagcgcg cgcgaacaag tgggcgaggt gatcgacacc
     7561 ctggccatgg ccaaagacca gtttcccggc aagcgcaaca acctggacgc gctgtgcgac
     7621 cgctttgaag tcgatcgctc caaccgggtg tttcacggcg cgctgattga ctgcgagctg
     7681 ctgggcgagg tgtacctggc catgacccga ggccaggaaa gcctgctgat ggacatcgag
     7741 ccgctcagca gcgagcgcgg cggcccgagc atgcgcctgg ccgacggccc gcgcccacgg
     7801 gtgcaagccg ccagcgccga agaactggcc gcccacgaag gctatctgga cgagctggac
     7861 aaagccatga aaggcccctg cctgtggcgg caaacgccgc cggaggcggc atgagcgcgc
     7921 gccgcctggc ggtggtgccg gccgccggca gcggcagccg ctttggccag gccaccccca
     7981 agcaatacac cctgctggcc ggccagccgc tgttggcgca caccctggcc gtgctgtgcg
     8041 ccgagccgcg cattgatcaa gtgtgggtgg tgatcagccc ggacgacgac tggttcgagc
     8101 catttggctt tgcgcaacag gcctgggcgc agtccaagct gcgcgtgctg cgccagggcg
     8161 gcgccacccg cgccgagagc gtgcgcaacg gcctggccgc gctggacgcc cagcccgacg
     8221 actgggtgct ggtgcacgac gccgcgcgct gctgcctgcc ggccgaagcg ctggcccggc
     8281 tgatcgacga gctggacgcc gacccctgcg gcgggctgtt ggcgctgccg gtggccgaca
     8341 ccctcaaacg ccaggacaag gacagccgcg tggccgccac cgtgccgcgc gacggcctgt
     8401 ggcaagcgca aaccccgcag atgttccgcc acggcctgct gtcgcaggcg ctggcaatca
     8461 gcagcagcgc cgacatcacc gacgaggcat ccgccatcga gcgcctgggt ctttgccccc
     8521 gcttggtgcg cggcgacgcc agcaacttca aagtgaccta tgctcaggat atccggctgg
     8581 ccgccgcctt gctgagcgcg ggccagaatt gagcgaacac acaggaaaca ggcaagatga
     8641 tccgcattgg acaaggttgg gacattcaca aactggtaga agggcgtccc ctgattctgg
     8701 gcggggtgac cattccgcac gacaagggct tgctgggcca ctctgacgcc gacgcgctct
     8761 tgcacgccat caccgacgcc ctgctgggcg cggccggcct gggcgacatc ggcagccatt
     8821 ttccggatac cgacccggcg tttcatggcg tggacagccg cattctgctg cgggaaaccg
     8881 ccgcccgcct gcgtgagcgc ggctggaaag tggtgaatgt ggacagcacg gtgattgccc
     8941 agcgcccgaa actggccggc catatcgccg ccatgcgcgc caatatcgcc accgacctgc
     9001 gcctgaaaat ggacgaagtg aacgtcaagg caaaaaccag cgaaaaactc ggcccgatgg
     9061 gccgcgaaga aggcattgcc gcccaggcgg tgtgcctgat cgaacgggcc tgaaccccgg
     9121 cgaatatggg ataatgccgg ccaatactgg atttccatag cgagaccccc atgagcaccc
     9181 tgacccaaga cgacaaaaaa cgcgccgtgg cgcgcaaagc cattgatttt gtgcccgacg
     9241 acagcatcat cggcgtgggc accggctcga cggtgaattt ctttatcgac gaactggccc
     9301 gcatcaaggc gcgcatcgac ggctgcgtgt ccagctcgga cgcctccacc gcccggctga
     9361 aagcccacgg catcccggtg tacgagctct ccagcgttgg cgagttgcag gtgtacatcg
     9421 acggcgccga cgaggtgaac cactcgctgc acatgatcaa gggcggcggc ggcgcgctga
     9481 cccgcgaaaa aatcgtcgcc tcggccgcgc gcgaattcat ctgcattgcc gacgaggcca
     9541 aatacgtcag caagctgggc gcttttccgg tggcggtgga agtcattccg atggcgcgct
     9601 cggtggtggc gcgcgagctg gtgcacctgg gcggccaccc ggaactgcgc cagggcttca
     9661 ccaccgacaa cggcaacctg attctggacg tgcacgatct ggacatcctc aagccgctgg
     9721 agatggaaac cgccatcaac caaatcgccg gcgtggtgtg ctgcggcatc tttgcccgcc
     9781 agcacgccca cgtgctgctg ctgggcgctg acgacggcgt caaagtgatc cgctgaccaa
     9841 aaaccgggcc aaaccggccg ccgccgcgcc aaaacagcct gcggcggcgc ctgtcggccc
     9901 gttatttgca tggatgtcat taaatattca tcttgcctcg caacaattgc acattcaccg
     9961 aacctcaatc ccaggaattc accatggcag agcatatttc caagcagttt gatctggagc
    10021 ttgaaaccat ccgcacccgc gtgttgcaga tgggaggcct ggtcgaggcg caaatcgtcg
    10081 gggcgatcga cggcctgatg tcgtcggaca tcgccaagct ggacaaggtg attgccgaag
    10141 acgcgctggt caacgccatg gaagtcagcc tggacgaaga atgtcagcac atcatcgccc
    10201 gccgccaacc ggccgccagc gacctgcgca tggtcatcac cgtgatcaag accatcaccg
    10261 atctggagcg catcggcgac gaggcgcaaa aagtggcgcg catgggcaag accattctgc
    10321 tggccgaccg cgtgcacatg ccgcgctttc gtgaaatcca gaaaatggcc gaagtggcgc
    10381 tggccatgct gcgccgcgcg ctggacgcct ttgcccgcct ggacgtggcc ggcgcgctgg
    10441 acgtggcccg tcaggaccgc aagctggacg aagaattcga cgccaacttc cgtcagttgg
    10501 tgaccttcat gatggaagac ccgcgcgcga tttcgatgtc gattgacacg attttcatgt
    10561 gcaaggcgat tgaacggatt ggcgaccacg ccaagaatat ttccgagtat gtggtgtatc
    10621 tggtcaaggg caaggatatt cgccacaccg cgctggaaga tgttgag
//
LOCUS       DAOPYX010000070        10663 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_7159_length_10663_cov_10.490196, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000070 DAOPYX010000000
VERSION     DAOPYX010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 10663)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10663)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10663
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7159_length_10663_cov_10.490196"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..185
                     /locus_tag="PKN55_05225"
     CDS             <1..185
                     /locus_tag="PKN55_05225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008952221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="iron transporter"
                     /protein_id="HNX70675.1"
                     /translation="IKLQGPGKYQLKLTVLPPSQNPHGHFGRHVDKETGVGPWFKPFE
                     LSYEFAFAGIGKKGGY"
     gene            248..580
                     /locus_tag="PKN55_05230"
     CDS             248..580
                     /locus_tag="PKN55_05230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014088541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HNX70676.1"
                     /translation="MKLTSLLIGGLGLLACLGAQAADMPTLRLELKDGVLNPARIQAP
                     AGVKFRLEIVNTGKTPVEFESTQLRKEKVLAPGAQSSVVIQPLTPGEYKFFDEFHMNT
                     AQGVIVAK"
     gene            678..1499
                     /locus_tag="PKN55_05235"
     CDS             678..1499
                     /locus_tag="PKN55_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008952223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HNX70677.1"
                     /translation="MEQVLFIVWRESVEALLVVGILHAWMNQNPQGRSGKPYLWGGVA
                     LGLLLAVALAVLLFVASGWMGAAQDAFQAGMVFVASALIVQMVLWMRAHGRTLKRELE
                     SGLAARAEQANWWGVTALAAIAIAREGSETVVFLYGSLSNASAADSAPLALAGLGGLA
                     LALLTFYVLQLGGRVLSWRLFFRVTEILLLLLGASLFLSGVEKLISMDYLPALLDPVW
                     DSSALIDDMSPAGGVLAALVGYRAQPALTSVLAYAGFWLAMAALMARGKRQAAAR"
     gene            1496..2911
                     /locus_tag="PKN55_05240"
     CDS             1496..2911
                     /locus_tag="PKN55_05240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008952224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNX70678.1"
                     /translation="MKARTVIPILAMPAAPAPSTGRLATVGRWLRDHATLLRRLQWAV
                     VLVYAALLVIPALLPLPDDSARMLNNLTIFAQFVFWGIWWPFVLLSMVLFGRIWCGVL
                     CPEGALTEWAAQKGLGRPIPRWMRWGGWPFVAFVLTTVYGQMVSVYQYPQAALLVLGG
                     STVAAVAVGWVYTKGKRAWCRHLCPVNGVFALLAKLAPMAYRVDEARWAASAHRTHSA
                     VNCAPLQPLRHMQGGSGCHMCGRCSDHRDAIQLQARSPADEVLRVAAKEADGWQTALI
                     VFGLLGVAIGAFHWTVSPWFVALKQGAAEWLIEHDILWPLDTDIPWWLLTHYPQYNDV
                     FSWLDGASLLAYVALTALAMGGGLLLCLAAAVALAGGWRRQRLHHLAQSLIPLAGCGV
                     FLGLSALTVTLLKAEGVDMHWANTLRLTLLTAASVWSAWLAWRILGQWSHGARRALAS
                     LPMLAALGWVGMAWGWMFWWW"
     gene            3055..3987
                     /gene="msrP"
                     /locus_tag="PKN55_05245"
     CDS             3055..3987
                     /gene="msrP"
                     /locus_tag="PKN55_05245"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018748376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016667 - oxidoreductase activity, acting
                     on a sulfur group of donors [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HNX70679.1"
                     /translation="MPLIHLPDPSAPLPSEITPPEVYRDRRRFMQAGLALATLPWLAE
                     SAQAGLAAQKSPLPTDEPLNKLSDITRYNNFYEFGVDKADPAVNAGSLRTSPWTVRVE
                     GAVQSPRTFDLDALMKLAPMEERVYRLRCVEGWSMVIPWIGFPLAALLKQVQPTAAGK
                     FVEFVTLHDPKQMPGQRQPVLEWPYREGLRIDEAMHPLTLLTFGLYGEVLPRQNGAPV
                     RLVVPWKYGFKSIKSIVAIRLVEKAPLTSWMQANPREYGFFSNVNPEVDHPRWSQASE
                     RRIGEFLKRKTLMFNGYAEQVAGLYRGMDLRKFF"
     gene            3987..4574
                     /locus_tag="PKN55_05250"
     CDS             3987..4574
                     /locus_tag="PKN55_05250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012697703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HNX70680.1"
                     /translation="MAARDWRGWALKAAVAVGCALPAVSLGWRLPQAVNPVEFLTRST
                     GTWALVGLLATLTVSPLRQLSGWAAWLRVRRLLGLSAFAYAVAHFVLYVWLDQGLEAA
                     AIWRDVLKRPFVTAGMAAFMLLLPLAITSTDGWMRRLKRRWGQLHQLVYPAAALAVLH
                     YLWLVKKDLTQPLLYALALAMLLGWRLWRWGRRQR"
     gene            4761..5720
                     /locus_tag="PKN55_05255"
     CDS             4761..5720
                     /locus_tag="PKN55_05255"
                     /inference="COORDINATES: protein motif:HMM:NF024772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNX70681.1"
                     /translation="MLQPLRGWLLLCLCLLCVACAPGGEAPLRVGTNIWLGYEPFYVA
                     RQAGSLPENVRLVEYASASEVLSAFRSRAIEAAAVTLDEAISLAENDNSLRIGLMLDT
                     SNGADALVARPGIRRLSELKGRTIGVESLAVGAYLLGRALQRADLSVHDVRIVPTPLD
                     QHESAFSRAEVDAVVTFYPVLAHLRERGGEVLFDSRAMPGEIVDVLVYREEQHKQHGK
                     ALQALALAWFDGVGRVADRQPQAMLTMAHRQKLSPRMLNEALALLEFQDITRNRSALL
                     GAHPGLAPVFGRLSLGMRELGLINRPLEPVNHLDSRIVEDLPG"
     gene            5717..8596
                     /locus_tag="PKN55_05260"
     CDS             5717..8596
                     /locus_tag="PKN55_05260"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNX70682.1"
                     /translation="MNVFHRFSLRLALPLTLLVMLTGTLALTVGHNLYQGRQRIEQQA
                     IGQVTQKLDQLHGLIADSLLTDDLPAVQHMVSMASADADVRMLLVVDGDGVVRAANRF
                     EWVGSPFSQVPGALPEALSYARTSDVSRIRLAPGGRWVVAQTRLLFAPEPGELRSSQV
                     GVLLMLYDLERAKQMAGRDSLLSALTDWLAIALMASVIYLLFHFSFNRRIQRLLTGIG
                     RLRDGDLSTRLALPGRDELAQVAHAVDDMAGQLATHQRELAAQQMAMAESSARYRALF
                     ESSLDGIVLIDEMGDVVDANPTFRRLAGLSQDVLVGRAFHRLLDDDSRADWNGPMREQ
                     LESRGYTDEFELKLLTVAGEPAAVATKCMRLADSRGVRRGGWCLLRDLSERKQAAIEQ
                     RLAAAVYDSSSEAILVTDPDFRVCMVNPAFVQHTGQDTVTLAGSVLPAFERGRDPRNS
                     AMLNALQRDGRWQGEHWMSRGNGETFPVWLSCAPALGDDGQVSHYIALFSDISERIAT
                     EQRMRYLAEHDELTGLANRFAFMQGLEARLDSAAPDSQLAVMFVDLDRFKPINDMMGH
                     KVGDDLLRRVAERITEVMGELAVVARQSADEFILFAPDVSAGEAEQLGQMMVGMLAQP
                     YRVGPYELSIAASVGVSCYPEHGLSASRLLKHAELAMSQAKRTGGSRALCYDPNMQEG
                     EEERHALEQDLRRAIERGDLLLEYQPQLNLDTREVSAVEALLRWRHPTLGWISPAKFI
                     PLAEETGLIQPIGQWVLKEACRQMADWHARDLPLTVAVNLSALQLRHAGLFNDVYQAL
                     AQSGVEAAFLELELTESALMENIDAMLDTLREFDELGVQLSIDDFGTGYSSLAYLRHL
                     PIGELKIDRSFVKDLETDPHAAPIVEAILAMAHSLGLSVVAEGVETDAQFAFLAAKGC
                     GYVQGWWLAKSMPAAELERWMAEYDIGAVAERISQARQPRAVLLG"
     gene            8772..9746
                     /locus_tag="PKN55_05265"
     CDS             8772..9746
                     /locus_tag="PKN55_05265"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HNX70683.1"
                     /translation="MAVSCSWESIDVHLLQVLHALLTECSVSNAARRLNQSQPAVSTA
                     LRRLRDITGDPLLVRSRNGMTPTERGQALLEPVKEALAHIEAIGVRQQRFDPAVSQRV
                     FNIATPDYLNAILIGRLVARLRALAPHAQVALHSFGPDHDYARALESGELDVVIGNWP
                     NPPEHLRLAPLFEDDVVCLMRKSHPLAGRALTPQDYLDADHLVPTPYAVGQRGVIDLQ
                     LARERLKRKVVASVPYFSLAPYVLLQNDILFSAPRLFAEHCCSLGELCMAESPLEFPK
                     VRFYLLWHDRAHHGEEGRWFREQVVDVIRETVGTLHAAREHTPRRARA"
     gene            complement(9948..>10663)
                     /locus_tag="PKN55_05270"
     CDS             complement(9948..>10663)
                     /locus_tag="PKN55_05270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011134431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HNX70684.1"
                     /translation="TCNFTAPVGDKPALFTHDEVITLFHEFGHGLHHMLTEVDELGVS
                     GINGVEWDAVELPSQFMENFCWEWDVLSHMTAHVDSGEPLPHELFDKMLAAKNFQSGM
                     MTVRQVEFGLFDMLLHAELQAKGEFMAVLEAVRAEVAVNPTPAYNRMAHTFSHIFAGG
                     YAAGYYSYKWAEVLSADAYAAFEETGHANPAVGEAFRREILAVGGSRPAIESFRAFRG
                     RDPQIDALLRHSGLVAEQA"
BASE COUNT         1702 a         3316 c         3623 g         2022 t
ORIGIN      
        1 acatcaagct gcaagggccg ggcaagtatc agctcaagct caccgtgctg ccgccctcgc
       61 aaaacccgca cggccacttt ggccgccatg tggacaagga aaccggcgtg ggcccgtggt
      121 tcaagccgtt tgagctgagc tatgagtttg cgtttgccgg cattggcaaa aaaggcggct
      181 attgatcttc gattgactgc ttgacgccgc gcggcgcacc cgatgcgccg cgccggaggg
      241 cgcactgatg aaactgacct ctttattgat tggcggcctg ggcctgctgg cctgtctggg
      301 cgcgcaggcg gcggacatgc ccaccctgcg gctggagctc aaggacggcg tgctcaaccc
      361 ggcgcgcatc caggccccgg ccggggtgaa gttccggctg gagatcgtca acaccggcaa
      421 gacgccggtg gagtttgaaa gcactcagct gcgcaaagaa aaagtgctgg cccccggcgc
      481 gcaatcgtcg gtggtgatcc agccgctgac gccgggcgaa tacaagtttt ttgacgaatt
      541 tcacatgaac accgcgcaag gcgtgatcgt cgccaaataa gcgccgcacg ccacccggcg
      601 ccgcttgcct gacgggcggg cgacggcaaa acgggttgtg tcagcgacgc tgagcgcgca
      661 gcaggtgggg cgcaagcatg gaacaggttt tgtttatcgt ctggcgtgaa agcgtcgagg
      721 cattgttggt ggtgggcatt ctgcacgcct ggatgaacca gaacccgcaa gggcgttctg
      781 gcaaacccta tttgtggggc ggggtggcgc tgggcctgtt gctggcagtg gcgctggcgg
      841 tgttgctgtt tgtcgccagc ggctggatgg gcgcggcgca ggatgcgttt caggcgggca
      901 tggtgtttgt cgcctcggcg ctgattgtgc agatggtgtt gtggatgcgc gcccatggcc
      961 gcacgctcaa gcgcgagctg gaaagcggcc tggccgcgcg cgccgaacag gccaactggt
     1021 ggggggtaac cgcgctggcg gccatcgcca ttgcccgcga aggcagcgaa accgtggtgt
     1081 ttctgtatgg cagcctgagc aatgccagcg ccgccgacag cgccccgctg gccttggccg
     1141 gcctgggcgg gctggcgctg gccttgctga ccttttatgt gctgcaactg ggcgggcgtg
     1201 tgctgtcgtg gcggctgttt ttccgggtga cagagatttt gctcttgctg ctgggcgctt
     1261 cgctgtttct gtctggcgta gaaaaactga tttccatgga ctacctgccc gccctgctcg
     1321 acccggtgtg ggacagcagc gcgctgattg atgatatgtc ccccgctggc ggcgtgctgg
     1381 cggcgctggt gggctatcgc gcccagccgg cgctgaccag cgtgctggcc tacgccggct
     1441 tctggctggc catggcggcg ctgatggcgc gcggcaaacg gcaggcggcg gcgcgatgaa
     1501 agcgcgcacg gtcatcccca tcctggccat gcccgccgcc ccggccccat ccaccgggcg
     1561 gctggcgacg gtgggccgct ggctgcgcga ccacgccacg ctgctgcgtc ggctgcaatg
     1621 ggcggtggtg ctggtgtacg ccgccttgct ggtgattccg gcgctgttgc cgctgccgga
     1681 cgacagcgcg cgcatgctga acaacctgac catcttcgcc cagtttgtgt tctggggaat
     1741 ctggtggccg tttgtgttgc tgtcgatggt gttgtttggc cgcatctggt gcggcgtgct
     1801 gtgcccggaa ggcgcgctga ccgaatgggc ggcgcaaaaa ggcctgggcc ggccgattcc
     1861 gcgctggatg cgctggggcg gctggccgtt tgtcgccttt gtgctgacca cggtgtatgg
     1921 ccagatggtc agcgtctatc aatacccgca agcggcgctg ctggtgctgg gcggctccac
     1981 cgtcgccgcc gtggcggtgg gctgggtgta caccaagggc aagcgcgcct ggtgccggca
     2041 tttgtgcccg gtgaatggcg tgtttgccct gttggccaaa ctggcgccga tggcctaccg
     2101 ggtggacgaa gcccgctggg ccgcctctgc ccaccgcacg cacagtgcgg tcaactgcgc
     2161 cccgctgcaa cccctgcgcc atatgcaggg cggcagcggc tgtcatatgt gcggccgctg
     2221 tagcgaccac cgcgacgcca tccagctgca agcccgctcg cccgccgacg aagtgctgcg
     2281 ggtggccgcc aaagaagccg acggctggca aaccgcgctg attgtgtttg gcctgctggg
     2341 cgtggccatc ggcgcgtttc actggacggt cagcccgtgg tttgtcgccc tcaagcaggg
     2401 cgcggcggaa tggctgatcg aacacgacat cctctggccg ctggacaccg acatcccctg
     2461 gtggctgctc acccattacc cgcaatataa cgacgtgttc tcctggctgg acggcgccag
     2521 cctgctggcc tacgtggcgc tgaccgcgct ggccatgggc ggcggcctgt tgctgtgcct
     2581 ggcggcggct gtcgcgctgg ccggcggttg gcgccgccag cgcctgcatc acctggcgca
     2641 aagcctgatt ccactggccg gctgcggcgt gtttctcggc ctgtcagcgc tgaccgtcac
     2701 cctgctcaag gccgaaggcg tggacatgca ctgggccaac accctgcgcc tgaccctgct
     2761 caccgccgcc agcgtctggt ccgcctggct ggcctggcgc atcctcggcc aatggagcca
     2821 cggggcccgc cgcgccctgg ccagcctgcc gatgctggcg gcgctgggct gggtcggcat
     2881 ggcctggggg tggatgttct ggtggtggtg aggagaataa gcggaacctt gtgtacgggc
     2941 tattctccaa gcgtggcctt acgcgccatg tcggcacgta aggttttgtc tggttggcgc
     3001 gacagaatgg cattgtcctc ttctgccgcg ccgccgcgct tctggagccc gatcatgccc
     3061 ctcatccatc tccccgaccc ttccgccccg cttccttccg agatcacccc gcccgaggtc
     3121 taccgtgacc gtcggcgttt catgcaggcc gggctggccc tggcgacttt gccgtggttg
     3181 gcggagtcgg cgcaggcggg tttggctgcg cagaaaagcc cgctgcctac tgatgagccg
     3241 ctgaacaagc tgtcggacat cacccgctac aacaattttt acgagtttgg cgtggacaag
     3301 gcggatccgg cggtgaatgc cggcagtttg cgcacttcgc cgtggacggt gcgggtggag
     3361 ggggcggtgc agtcgccgcg cacgtttgat ctggatgcgc tgatgaagct ggcgccgatg
     3421 gaggagcggg tttaccggct gcgctgtgtc gaggggtggt cgatggtgat tccgtggatt
     3481 ggttttccgc tggcggcctt gctcaagcag gtgcagccga cggcggcggg caagtttgtc
     3541 gagttcgtca ccctgcacga ccccaagcag atgcctggcc agcgccagcc ggtgctggag
     3601 tggccgtatc gcgaggggct gcgcattgac gaggcgatgc atccgctgac tttgctgacc
     3661 tttggtttgt atggcgaggt gttgcctcgg cagaatggcg cgccggtgcg cttggtcgtg
     3721 ccgtggaagt atggcttcaa gagcatcaag tcgattgtcg ccatccggct ggtggaaaaa
     3781 gccccgctga ccagctggat gcaggccaat ccgcgcgagt atggcttttt cagcaatgtg
     3841 aatccggagg tggatcatcc acgctggagc caggccagcg agcggcggat tggcgagttt
     3901 ctcaagcgca aaacgctgat gttcaatggc tacgccgagc aggtggcggg gctgtatcgc
     3961 ggcatggatt tgcggaagtt tttttgatgg cggcgcgtga ttggcgtggg tgggcgctga
     4021 aagcggcggt ggcggtgggg tgcgcgctgc cggcggtgtc tttgggttgg cgcttgccgc
     4081 aggcggtgaa cccggtggag tttctgaccc gaagcacggg cacgtgggcg ctggtggggt
     4141 tgttggcgac gttgacggtc agccccttgc ggcagctcag cggctgggcg gcgtggctgc
     4201 gtgtgcgtcg tttgttgggg ttatcggctt ttgcctatgc cgtggcgcat ttcgtattgt
     4261 atgtctggct ggatcagggg ctggaggcgg cggcgatctg gcgcgatgtg ctcaagcggc
     4321 cgtttgtcac cgctggcatg gcggcgttta tgctgctgct tccgctggcc atcacctcga
     4381 ccgatggctg gatgcgccgg ctcaagcggc gctgggggca gttgcaccag ctggtatatc
     4441 cggccgcagc gctggcggtg ctgcattatc tctggctggt gaaaaaagac ctcactcagc
     4501 cgctgctgta cgcgctggcg ctggcgatgt tgctgggctg gcggctgtgg cgctgggggc
     4561 ggcgccagcg ttaggccact ggccggatgc atccggcccg cgtggccggc ctggcgtctg
     4621 gatggatgcc gtgcgcggca aggcactcat actgcatgaa atgtgtggac ccctgcacgc
     4681 caaactggct taaatattgg aaattcggcc aaagactaca gtagaatccc gcagactaca
     4741 ctgcgattgg cgctcctctc atgttgcaac ccctgcgcgg ctggctgttg ctgtgcctgt
     4801 gtctgctgtg tgtggcctgc gcccctggcg gcgaagcgcc gctacgggtg ggcaccaata
     4861 tctggctggg ctatgagccc ttttatgtgg cacgtcaggc tggcagcctg cccgagaacg
     4921 ttcggctggt ggaatatgct tcggccagcg aggtgctcag cgcctttcgc agccgggcca
     4981 tcgaagccgc cgccgtcacg ctggatgaag ccatcagtct ggccgaaaac gacaacagcc
     5041 tgcgcattgg cctgatgttg gatacctcga atggtgcgga tgcgctggtg gcgcgcccgg
     5101 gcatccgccg gctgagcgaa ctcaaagggc gaaccatcgg ggtggaaagc ctggcggttg
     5161 gcgcgtattt gctgggacgg gcgctgcaac gtgctgattt gtcggtgcac gacgtccgca
     5221 tcgtccctac cccgctggat caacacgaaa gcgcgttttc tcgtgccgaa gtggatgccg
     5281 tggtgacgtt ttatccggtg ctggcgcatc tgcgcgagcg cggtggagag gtattgttcg
     5341 acagtcgtgc catgcccggc gagattgtcg atgtgctggt gtaccgcgaa gagcagcata
     5401 agcagcatgg caaggccttg caggcgctgg cgctggcctg gtttgacggc gttggccgtg
     5461 tagctgatcg gcagccgcag gccatgctga ccatggccca ccggcaaaag ctgtcgccac
     5521 gcatgctgaa cgaggcgctg gccctgctgg agtttcaaga catcacccgc aaccgtagcg
     5581 ccttgctggg cgcacatccc gggctggcgc cggtatttgg ccggctgagc ctggggatgc
     5641 gcgagctggg cctgatcaac cggccacttg agccggtcaa ccatctagat agcaggattg
     5701 tcgaggattt gccaggatga atgtgtttca ccgcttctcg ctgcggctgg cgctgccgct
     5761 gacgctgctg gtcatgctga ccggcacgtt ggcgttgacg gtgggacaca atctttatca
     5821 gggccgacaa cgcatcgaac agcaggccat cgggcaagtc acccaaaaac tggatcaact
     5881 gcacggcctg attgccgaca gcctgctgac cgacgatttg cccgccgtgc agcacatggt
     5941 atccatggcc agcgccgacg ccgacgtgcg catgctgctg gtggtggatg gcgacggtgt
     6001 ggtgcgtgcc gccaaccgct tcgagtgggt gggctcgcct ttctcgcaag tgcccggcgc
     6061 gctgcccgag gcattgagct atgcccgcac ttctgacgtc agccgcatcc ggctggcgcc
     6121 cggtggccgc tgggtggtgg cgcaaacccg cctgctgttt gccccagaac ccggcgagct
     6181 gcgctccagc caggtaggcg tattgctgat gctttacgat ctggagcgcg ccaagcagat
     6241 ggccgggcgg gattccctgc tgtcggcgct caccgactgg ctggccattg ccctgatggc
     6301 cagtgtgatt tatttgctgt tccatttttc tttcaatcgc cgcatccagc gcctgctgac
     6361 cggcattggc cgcctgcgcg atggcgattt gtccacccgg ctggcgctgc ccggccgtga
     6421 cgagctggcg caagtggcgc atgcggtgga tgacatggcc ggccagttgg ccacccacca
     6481 gcgcgaactg gctgcccagc aaatggccat ggcggaatcc tcggctcgct accgcgccct
     6541 gtttgaatcc agcctggacg gcattgtgct gatcgacgaa atgggcgatg tggtggacgc
     6601 caatcccacc ttccggcggc tggccgggct gagccaggat gtgctggtgg ggcgggcatt
     6661 tcatcgcctg ctggatgacg acagccgcgc cgactggaac ggcccgatgc gtgaacagct
     6721 ggaaagccgt ggctacaccg acgaattcga actcaagctg cttaccgttg ccggcgagcc
     6781 agcggccgtg gcaaccaaat gcatgcgtct ggccgacagc cggggcgtgc ggcgcggcgg
     6841 ctggtgcctg ctgcgcgatt tatccgagcg caagcaagcg gccatcgagc agcggctggc
     6901 cgccgccgtg tacgacagca gctccgaggc cattttggtc accgatccgg atttccgggt
     6961 gtgcatggtc aatccggcct ttgtgcagca taccggccag gatacggtga cgctggccgg
     7021 cagcgtgctg cccgccttcg agcgcggccg cgacccgcgc aacagcgcca tgctcaatgc
     7081 cctgcaacgc gatggccgct ggcagggcga gcactggatg agccggggca atggcgaaac
     7141 cttcccggtg tggctgtcct gcgcacccgc gctgggcgat gatggccagg tcagccacta
     7201 tatcgccctg ttctccgaca tcagcgagcg catcgccact gagcagcgca tgcgctatct
     7261 ggccgaacac gacgaactca ccggcctggc caaccgcttt gcctttatgc aagggctgga
     7321 ggcgcggctg gacagcgccg ccccggacag ccagctggcg gtgatgtttg tcgatctgga
     7381 ccgcttcaag ccgatcaacg acatgatggg ccataaagtc ggcgacgatc tgctgcgccg
     7441 ggtagccgaa cgcattaccg aagtgatggg cgaactggcg gtggtggcgc ggcaatcggc
     7501 agatgaattt atcctgtttg ccccggacgt ttccgctggc gaagccgagc agctggggca
     7561 aatgatggtg ggcatgctgg cccagccata ccgggtaggc ccttacgagc tgtcaattgc
     7621 cgcctcggtc ggggtcagct gctatcccga acacggcctg agcgcctcgc gcctgctcaa
     7681 gcacgccgag ctggccatga gccaggccaa acgcaccggc ggcagccgcg cgctgtgcta
     7741 cgacccgaac atgcaggaag gcgaagaaga acgccacgcg ctggagcaag acctgcgccg
     7801 cgccatcgag cgcggcgacc tcttgctgga ataccagccg caactgaatc tggacactcg
     7861 ggaggtcagc gctgtggaag ccctgttgcg ctggcgtcac ccgacactgg gctggatttc
     7921 acccgccaaa ttcattccac tggccgaaga aaccggcctg atccagccga ttggccaatg
     7981 ggtgctgaaa gaagcctgcc ggcagatggc cgactggcac gcccgcgatc tgccgctgac
     8041 cgtggcggtg aacctgtctg ccctgcaact gcgccacgcc gggctgttca acgatgtgta
     8101 tcaggcgctg gcgcaatccg gcgtagaggc cgcttttctg gagctggagc tgactgaaag
     8161 cgcgctgatg gaaaacatcg acgccatgct cgacaccctg cgcgaatttg acgagctggg
     8221 cgtgcaactc tccatcgacg actttggcac cggttactcc agcttggcct atctgcgtca
     8281 cctgccgatt ggcgagctga aaatcgaccg ctcgtttgtc aaagacctgg aaaccgaccc
     8341 gcacgccgcg ccgattgtcg aggccatcct ggccatggcg cattcgctgg gcctgagcgt
     8401 ggtggccgaa ggcgtggaaa ccgacgccca gtttgccttc ctggcggcca agggttgcgg
     8461 ctatgtgcaa ggctggtggc tggccaaatc catgccggct gccgagctgg agcgctggat
     8521 ggccgaatac gacatcggcg cggtagccga acgcatcagc caggcccgcc agccgcgcgc
     8581 cgtgctgctg ggctgagcgt cgctcacaca accccgcgcc gcgattctgg taaggcgtgc
     8641 ggccgcagcc aatacaagca gcgcaacgac gtccggaagg tttggtgagc ggttacgccc
     8701 gcccgcgccc aaagacaagg cccgcttatt gcttgtccca gacgtccgaa ttagcgccac
     8761 acgagggaaa catggccgtc agctgctcct gggaatccat cgacgtgcat ctcttgcagg
     8821 tgctgcacgc cctgctcacc gaatgcagtg tctctaacgc cgcgcgccgg ctgaatcagt
     8881 cacaaccggc agtcagcacc gcgctgcgcc gcctgcgcga catcaccggc gatccgctgc
     8941 tggtgcgcag ccgcaacggc atgactccca ccgaacgcgg ccaggcgctg ctggaaccgg
     9001 tcaaggaggc gctggcgcat atcgaagcca ttggcgtgcg ccagcaacgc tttgacccgg
     9061 ccgtctccca acgggtgttc aatatcgcca ccccggatta cctcaacgcc attctgattg
     9121 gccggctggt ggcgcgtctg cgcgcgctgg cgccacacgc ccaggtggcg ctgcactcgt
     9181 ttggcccaga tcacgactac gcccgcgcgc tggaatccgg cgagctggac gtggtgattg
     9241 gcaactggcc caacccgccc gagcatctgc gcctggcgcc gctgtttgag gacgatgtgg
     9301 tgtgcctgat gcgcaaaagc cacccgctgg cgggccgggc gctgaccccg caagactatc
     9361 tggacgccga ccatctggtg cccaccccgt atgccgttgg ccagcgtggg gtgatcgacc
     9421 tgcaattggc gcgcgagcgg ctcaagcgca aggtagtggc cagcgtgccc tacttcagcc
     9481 tggcgccgta tgtgctgctg caaaacgata ttttgttctc ggcgccgcgc ttgtttgccg
     9541 aacattgctg ttcgctgggt gagctgtgca tggctgaatc gccgctggag tttcccaagg
     9601 tgcgctttta tctgctgtgg cacgaccgcg cccaccacgg cgaagaaggc cgctggtttc
     9661 gcgagcaggt ggtggacgtg attcgtgaaa ccgtcggcac gctgcatgcc gcccgtgagc
     9721 acacgccccg gcgcgcccgc gcctgaagcc gtttcctgct ccgggggcaa catgtcgctg
     9781 tcggcacgcg tgaccctcaa ggggcgcgtc gcgttgccag agacaaaaac ctcggtagac
     9841 acaggcaagg aaggcgcctt gcgccaccag gtgcgcctgt cgatggatag aaagtgggcg
     9901 ccccggtttc tggtgcgccc acatcgccac gcggcctgat ggccgcctta tgcctgctcg
     9961 gcgaccaggc cgctgtggcg cagcagggcg tcgatttgcg ggtcgcggcc gcggaaggcg
    10021 cggaaagact caatcgccgg gcgcgagccg cccacggcca gaatctcgcg gcggaaggcc
    10081 tcgcccaccg ccgggttggc gtggccggtt tcttcaaacg ccgcgtaggc gtcggccgac
    10141 agcacttccg cccacttgta gctgtagtag cccgccgcgt agccgccggc aaagatatgg
    10201 ctgaaggtgt gcgccatgcg gttgtaagcc ggggtcgggt tgaccgccac ttcggcgcgc
    10261 accgcctcca gcaccgccat aaactcgccc ttggcttgca gctcggcgtg caacagcatg
    10321 tcaaacaggc cgaactccac ctgacgcacg gtcatcatgc cggactggaa attcttggcc
    10381 gccagcatct tgtcgaacag ctcgtgcggc agcggctcgc cgctgtccac atgggcggtc
    10441 atgtgcgaca gcacgtccca ctcccagcag aagttctcca tgaactggct gggcagctcg
    10501 acggcgtccc attccacgcc attgatgccg gacacgccca gctcgtccac ctcggtcagc
    10561 atatggtgca ggccgtgacc aaactcatgg aacaaggtga tgacttcgtc gtgggtgaac
    10621 aaggccggct tgtcgcccac cggggcggtg aagttgcagg tca
//
LOCUS       DAOPYX010000071        10605 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_7222_length_10605_cov_9.406351, whole genome shotgun sequence.
ACCESSION   DAOPYX010000071 DAOPYX010000000
VERSION     DAOPYX010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 10605)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10605)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10605
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7222_length_10605_cov_9.406351"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..838)
                     /locus_tag="PKN55_05275"
     CDS             complement(<1..838)
                     /locus_tag="PKN55_05275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014087917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrkA family serine protein kinase"
                     /protein_id="HNX70685.1"
                     /translation="MNADIFASFASRYDRSREEEYSLKEYLEICRRDASAYATAAERM
                     LMAIGQPELIDTRHDSRLSRIFSNKVLKVYPAFREFYGMEDAIEQVVAYFRHAAQGLE
                     EKKQILYLLGPVGGGKSSIAEKLKELMQQVPFYCIKGSPVNESPLGLFNADEDGAILE
                     EEFGIPRRYLKTIPSPWAVKRLHEFNGDINQFRVVKRYPSVLRQIAVAKTEPGDENNQ
                     DISSLVGKVDIRKLEKYAQDDPDAYSYSGGLCLANQGLLEFVEMFKAPIKVLHPLLTA
                     TQE"
     gene            1195..1392
                     /locus_tag="PKN55_05280"
     CDS             1195..1392
                     /locus_tag="PKN55_05280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX70686.1"
                     /translation="MKLMSSGMPVSEEDTQAIDALSDKHKSWDSHSGSFAVHFFRLTD
                     QITLFGAAMPQTCAIRQTQKF"
     gene            complement(1533..1784)
                     /locus_tag="PKN55_05285"
     CDS             complement(1533..1784)
                     /locus_tag="PKN55_05285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX70687.1"
                     /translation="MNKLTSALIATVFGLSAAAGFAADAAKPEAAAKPAVEQKAEAKP
                     EAAKPAAKPAKVKKAKKAAKKPAAEASKDAAAKPEAAKQ"
     gene            complement(1876..3216)
                     /gene="rstB"
                     /locus_tag="PKN55_05290"
     CDS             complement(1876..3216)
                     /gene="rstB"
                     /locus_tag="PKN55_05290"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF007898.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HNX70688.1"
                     /translation="MLRVFIRFYLTLVVSFIGAIVLAGAIYGQVVANVSDRYLNDIFR
                     ATVSLVQTELADQPIERWNAKLETLSLSLPYPIKVEPLDTYRLSDSNREALTDGDIVM
                     LTDSYLFLQRVPDTGYMLTLGPIDYLYFLREIQSFDYLLLGLAALFLGVPAFLWMRPL
                     WRQLTHLKWVARQLGEGNLNARAKLPDDSGVAELGSAFNGMADNLQSLIQSRRELLNA
                     VSHELRTPIARLRYRLAMLEEGVADDVRSAMDRDISSIDKMIEELLLYSRLEQARDVL
                     QPQTLTLWPWLNSVVAQHAADFPDTDYQLTAVASTLHALTVDADPFYLERALSNLLRN
                     AARYGQGRVEVSLGADERQCWIKVADNGPGIPEGERERVFEPFVRLDRSRDRRTGGYG
                     LGLAIVQRIMGWHHGQASVATADLGGACFTLCWPRVHVVTSGHTSLTDEGESAA"
     gene            complement(3216..3941)
                     /gene="rstA"
                     /locus_tag="PKN55_05295"
     CDS             complement(3216..3941)
                     /gene="rstA"
                     /locus_tag="PKN55_05295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008953490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HNX70689.1"
                     /translation="MSPRILFVEDDLELAGLIGEFLTRNGFVLHHIDRGDLVEAAIAE
                     HDPDLLLLDIMLPGKDGLSICRDLRPGFTRPIILLTSVGSDMNQVLGLELGANDYVIK
                     TTPPAVLLARIRAQLRQHEAQGGTAPARPSAAAPANILHFGRLVVDAANRDVKLDGHS
                     VILSTSDFDLLWLLASHAGEILSRDTLLKALRGVEYDGLDRSIDVAVSRLRKKLGDDP
                     NEPHKIKTVRNKGYLFVRAGWDA"
     gene            4286..5512
                     /locus_tag="PKN55_05300"
     CDS             4286..5512
                     /locus_tag="PKN55_05300"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012696291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HNX70690.1"
                     /translation="MALIVQKYGGTSVGTTDRIKNVARRVAKWQAQGHQVVVVVSAMS
                     GETNRLIAMAREIQANPDPRELDVIASTGEQVTIGLLSMALKDIGVDAVSYTGWQMPM
                     ITDSAYTKARIQSIDDKRMRADLDAGRVVVVAGFQGIDESGSLTTLGRGGSDTSAVAI
                     AAALKADECQIYTDVDGVYTTDPRVVPEARKLSTITFEEMLEMASLGSKVLQIRSVEF
                     AGKYKVKLRVLSSFEEEGEGTLITFEEDENMEKAVVSGIAFDRNEARINVKGVPDKPG
                     IAYQILGPVADANIEVDMIIQNMGESGTTDFSFTVPRGEYQKALTILRDVQTHVGAAK
                     VDGDDKIAKVSIIGVGMRSHTGIASTMFRTLAEEGINIQMISTSEIKVSVLIDEKYLE
                     LGVRVLHKAFGLDQAQ"
     gene            5573..5666
                     /locus_tag="PKN55_05305"
     tRNA            5573..5666
                     /locus_tag="PKN55_05305"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5607..5609,aa:Ser,seq:gct)
     gene            5673..5749
                     /locus_tag="PKN55_05310"
     tRNA            5673..5749
                     /locus_tag="PKN55_05310"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5707..5709,aa:Arg,seq:acg)
     gene            5754..5829
                     /locus_tag="PKN55_05315"
     tRNA            5754..5829
                     /locus_tag="PKN55_05315"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5788..5790,aa:Glu,seq:ttc)
     gene            6109..7917
                     /locus_tag="PKN55_05320"
     CDS             6109..7917
                     /locus_tag="PKN55_05320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012696493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative nucleotidyltransferase substrate
                     binding domain-containing protein"
                     /protein_id="HNX70691.1"
                     /translation="MSRFDFLHPPFDVLTAGERDQVEKAVDIVFHPNEDVILAPGQSV
                     DALYMVIKGIVREVSDDGEIIAVYHSNDTFDARALIAGQSVHRFEVHEEALVFSLPKE
                     LVMGLIESNPVFGAFFFQSVSEKFSAMAQRSGNRELQTLLTATVREACVKTPVFLDGA
                     QTVLDAAQAMKRHRAKSILVRAGERIGIFTASDFRDIILTGTPASATLADTAHFNLVT
                     TDAGDYLFNALLTMTRHNIQRVVVTENGQPIGVLEQIDLLSYFSNHSHLIAQQIELAD
                     SLDTLKSVAAQIDRLVGVLSSHGVKAPQLARLVQTLNAQLFARAWKLIAPEELVENSC
                     LIVMGSEGRGEQIIKTDQDNALILRDGFNHPYLEDACARFSEALIEFGYPPCPGQIMV
                     NNPLWRKPQGEFQDQLYRWVYLPDADAQMNLAIFVDAEAVAGDAALLLQAKDYLRSIM
                     SDDVGFFLRFARAIEQFDTPLGLFSQLLTKSQGGVATLDLKKGGIFPIVHGIRAMALE
                     YKLEASNTLERIKLLVDQGNLESQLGHDTAEALSFLLEMRLKAGLDMLANGKTPSNQI
                     QPDRLSTLERDLLKDALAVVKRFKTTIRHHFKMTGF"
     gene            7919..8530
                     /locus_tag="PKN55_05325"
     CDS             7919..8530
                     /locus_tag="PKN55_05325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014088042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HNX70692.1"
                     /translation="MLAALKTAWARKRLSSPDYAHLFDGHPDEMVSVDCETTSLNVRE
                     AELLSIGAVRIRGNRVLTSESFYMLVKPERLPEGASVAVHGLRPIDVSNGVPPMEAVK
                     ALLDFIGGRPLVGYYLEYDVGVLNKYVKGLLNIGLPQQQIEVSGLYYDWKLKQNPDAY
                     IDLRLQPMLDELEIPTLARHDALNDAITAAMILLAMRQRGAKV"
     gene            complement(8661..10562)
                     /gene="htpG"
                     /locus_tag="PKN55_05330"
     CDS             complement(8661..10562)
                     /gene="htpG"
                     /locus_tag="PKN55_05330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014086119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HNX70693.1"
                     /translation="MSTQKETLGFQAEVKQLLQLMIHSLYSDKEIFLRELISNASDAA
                     DKLRFEALDKPDFYEQDSELRIRLSVDREARTLTLIDNGIGMSRDEVIDNIGTIAKSG
                     TRAFFEKLTGDARKDANLIGQFGVGFYSAFIVADKVTLTTRRAGLPASEGVRWESSGE
                     GDYTLETTDAPRGTHIVLHLREGEDELLDEWRLKQIIRKYSDHITLPIEMPTAAVTDE
                     DGKETQPAGWETVNKASALWARAKSEITDEEYKAFYQHVAHDYSDPLAWTHARVEGRQ
                     EYTQLLYLPARAPFDLYDRDARHGVKLYVRRVFIMDDADKLMPQYLRFVRGVIDSADL
                     PLNVSRELLQGNKDIDAIRAGCVKKVLAMLEDLAENQADKFATFWKEFGQVLKEGVGE
                     DFANRERIAKLLRFASTHTGNDGQTVSLADYLGRMKPEQDKIYYLTADSYTAALASPH
                     LEVFRKKGVEVLLLTDRVDEWVTSHLTEFDGKNLVSVARGALDLGALEDESEKASQKA
                     SEEAMKPLVDALSDTLKDTVKEVRVTHRLTESPACLVVDEFAMSNHLQRMLKAAGQDV
                     PVSKPILEINPEHPLVKKLDASRSQDTRFADLAHIVFDQALLAEGGQLSDPAGFVKRI
                     NALMLELSA"
BASE COUNT         2121 a         3228 c         3154 g         2102 t
ORIGIN      
        1 cttcctgggt ggcggtcagc agcggatgca gcaccttgat cggcgccttg aacatttcca
       61 caaattccag caagccctgg ttggccaggc acaggccgcc ggaatagctg taggcgtccg
      121 ggtcgtcctg ggcgtatttt tccagcttgc gaatgtccac cttgcccacc aggctggaaa
      181 tatcctggtt gttttcgtcg cccggctcgg tcttggccac ggcgatctgg cgcagcaccg
      241 aggggtagcg cttgaccacg cggaactgat tgatgtcgcc gttgaattca tgcagccgct
      301 tcaccgccca cgggctgggg atggttttca ggtagcggcg cggaatgccg aattcttctt
      361 ccagaatcgc gccgtcttca tcggcgttga acagccccag cggcgactcg ttcaccggcg
      421 agcccttgat gcaataaaac ggcacctgct gcatcagctc tttgagtttt tcggcaatcg
      481 acgacttgcc gccgcccacc gggcccagca aatacagaat ctgctttttc tcttccaggc
      541 cctgcgcggc atggcggaag taggccacca cctgctcgat ggcgtcttcc atgccgtaaa
      601 actcgcggaa ggccggatac actttcagca ccttgttgct gaagatgcgc gacaggcggg
      661 agtcatgccg ggtgtcgatc agctcgggct ggccgatggc catcaacatg cgttcggccg
      721 ccgtggcgta ggcggatgcg tcccgacggc agatctccag atattccttc agcgagtatt
      781 cctcttcccg actgcggtca tatcggctgg caaaactggc aaagatatcg gcgttcatga
      841 tggagctcct agacagctgc gatgcatggg tgcgggcggg caagggggca agccccatcc
      901 cctcagtaga gaggggaacc ttacttttgg tatagcatgt cgtcacgtga tagcccagtg
      961 aatatgctta tggcaaataa agcaaagcca ccggatgaaa acagcgtcaa agtcggatga
     1021 gcgaacctcc ctgtctgtac ggggaacgag ctgaccatgc cctgtgcgcc gtccgcgcgt
     1081 cgggtgtcgg cctttgataa agatggtgtg tcctgattgg tcccagcaaa acccgcgcca
     1141 gtcttcatgg actggcagga gccaggcatt ggcacggcaa gacttggaaa acacatgaaa
     1201 ctcatgtcgt caggcatgcc ggtttctgaa gaagatacac aggctataga cgctttgtcg
     1261 gacaaacaca aatcatggga tagccacagt ggctcgtttg cagtgcattt ttttcgtctg
     1321 accgaccaga tcacgttgtt tggcgcagca atgccccaaa cctgtgcaat acggcagaca
     1381 caaaaattct gaagttttcc ctggaatgac cccgccaaaa aaagaaggac gtcagatgac
     1441 gtccttgcaa gcggagaaaa accacaggaa acacaatctg gaggagcaga gcgccacgcg
     1501 tagatcagcg cgtggcggag agcacatact gcttactgct tggcagcttc cggcttggca
     1561 gccgcatcct tgctggcctc agcggccggc ttcttggcgg cttttttggc ctttttcact
     1621 ttagccggct tggcggccgg tttggcggct tccggcttgg cttctgcttt ttgctcgacg
     1681 gccggcttgg cggcggcttc cggcttggca gcatcggcag caaagccggc ggcggcggac
     1741 aggccaaaca cggtggcgat cagggcgctg gtcagtttgt tcatggcgga catcctttca
     1801 aaattcagag gcaatcaagc gggtttcggt atgaggacaa caccgcgtgc tgtgtgctgt
     1861 gttgttgaaa ccagattacg ccgccgactc cccctcgtct gtgaggcttg tgtgtccgga
     1921 tgtaaccaca tgtacacgcg gccagcacaa ggtgaagcat gcgccgccca agtccgccgt
     1981 cgccacgctg gcctggccgt gatgccagcc catgatgcgc tgaacaatcg ccagccccag
     2041 gccataaccg ccggtgcgcc ggtcgcgact gcgatccagc cgcacaaagg gctcgaacac
     2101 ccgctcccgc tcaccttccg ggatgcctgg gccattgtcg gccaccttga tccagcattg
     2161 tctctcgtca gcacccaggc tgacctcgac ccgaccttgc ccataacgtg cggcattgcg
     2221 cagcagattg gacagcgcgc gctccagata aaacggatcg gcgtctaccg tcagggcatg
     2281 cagcgtcgac gcaacggcag tcagttggta atcggtatca ggaaaatccg cggcgtgctg
     2341 cgccaccacg ctgttcagcc atggccacag cgtcagcgtc tgcggttgca gcacatcgcg
     2401 tgcttgctcc agacgggaat acagcaacag ctcttcaatc atcttgtcga tgctggaaat
     2461 atcccgatcc atggcgctgc gcacatcgtc ggccacgcct tcttccagca tggccagccg
     2521 gtagcgcagg cgggcaatgg gcgtgcgcag ctcgtgtgac acggcattga gcagctcgcg
     2581 acgggattgg atcaaggatt gcagattatc tgccatgcca ttgaatgcag agcccagttc
     2641 ggccacgccg gaatcatccg gcagcttggc gcgggcattg agattgcctt cgcccaactg
     2701 tctggccacc catttcagat gggtgagctg tcgccacagg gggcgcatcc agagaaaggc
     2761 cggcaccccc aaaaacaaag cggccagccc cagcagcaga taatcaaacg actggatttc
     2821 gcgcaaaaaa tacaaatagt caatcggccc cagcgtcagc atatagccgg tgtccggcac
     2881 ccgctgcaaa aacagatagc tgtcggtcag catgacaata tcgccatcgg tcaatgcttc
     2941 gcggttgctg tcgctgagcc ggtaggtgtc cagcggctcg accttgatcg gatagggcaa
     3001 tgacaggctg agagtttcca gcttggcgtt ccagcgctca atcggctggt cggccagctc
     3061 ggtttgcacc aggctgacgg tggcgcgaaa aatatcgttc agatagcggt cactgacatt
     3121 ggccaccacc tggccataaa tggccccggc cagcacgatg gcgccgataa aactgaccac
     3181 cagggtcaga taaaagcgga tgaatacgcg cagcatcagg catcccagcc ggcgcgcaca
     3241 aacaaatagc ccttgttgcg cacggtcttg atcttgtgcg gctcgttggg gtcatcgccc
     3301 agttttttgc gcaagcgcga caccgccacg tcaatgctgc ggtccagccc gtcgtactcc
     3361 accccgcgca gcgccttgag caaggtatca cgggacagga tttctccggc gtggctggcc
     3421 agcagccaca gcaaatcaaa atcgctggtg gacaggatca cgctgtggcc gtccagtttg
     3481 acgtcgcggt tggcagcatc caccaccagg cggccaaagt gcaggatatt ggccggcgcg
     3541 gcagcggacg ggcgcgccgg ggcggtgccg ccttgcgcct cgtgctggcg cagttgggcg
     3601 cggatgcgcg ccagcagcac cgctggcggc gtggtcttga tcacataatc attggcgccc
     3661 agctccagcc ccagcacctg gttcatgtcg ctgcccaccg aagtcagcaa gataatgggg
     3721 cgggtgaagc cggggcgcag gtcgcggcag atggacaggc catctttgcc gggcagcatg
     3781 atgtccagca gcagcaaatc cgggtcgtgc tcggcaatcg ccgcttcgac cagatcgcca
     3841 cggtcgatat ggtgcagcac gaagccgttg cgggtgagaa attcaccgat cagtccggcc
     3901 agttcaagat cgtcttcaac aaacagaatg cgcggggaca tggacgtgac agggagcaaa
     3961 aagtcagcaa ggcggccagt gtagcgccgc tgcgcgctgc cgagaaggcg ggcgcatgcc
     4021 gaaagcaagc ccgtttgccg cgtttgcaca taagcggctc tcgtattggt cgggcaattt
     4081 ggttaaaatc atgggctttc accgcttcaa ccgcctgatg gcggtgtcaa gcgttgctga
     4141 gccagccggt tgtggccggt ttgctcagcg gcgtttcgca gcgccttgca tcgtgtcgcc
     4201 gccggcaaaa ctggctcgcg aagcgcatcg ggcacactgt gggccgtgac gttttttcat
     4261 tttcactatc aagaagctac aagcaatggc tcttatcgtt cagaaatacg gcggcacgtc
     4321 ggtgggcacc actgaccgca tcaagaacgt cgcccgccgc gtggccaaat ggcaggccca
     4381 aggccaccag gtggtggtgg tggtgtccgc aatgagcggc gaaaccaacc gcctgatcgc
     4441 catggcccgt gaaatccagg ccaaccccga tccgcgcgaa cttgacgtga tcgcctccac
     4501 cggcgaacag gtcaccatcg gcctgctgtc gatggcgctc aaggacatcg gcgtggacgc
     4561 ggtcagctac accggctggc aaatgccgat gatcaccgac agcgcctaca ccaaggcccg
     4621 catccagtcg atcgacgaca agcgcatgcg cgccgatctg gacgctggtc gcgtggtggt
     4681 ggtggccggt ttccagggca tcgacgaatc cggcagcctg accacgctgg gccgtggcgg
     4741 ctcggacact tccgccgtgg cgattgccgc cgcgctgaag gccgacgaat gccagattta
     4801 caccgacgtg gatggcgtgt acaccaccga tccgcgcgtg gtgccggaag cccgcaagct
     4861 gtccaccatc accttcgaag aaatgctgga aatggccagc ctgggttcga aagtgctgca
     4921 aatccgctcg gtggaattcg ccggcaagta caaagtcaag ctgcgcgtcc tgtcctcctt
     4981 tgaagaagag ggtgagggca cgctgattac gtttgaggaa gacgaaaaca tggaaaaagc
     5041 cgttgtctcc ggcattgcct ttgatcgcaa cgaagctcgc atcaacgtca agggcgtgcc
     5101 tgacaaaccg ggcatcgctt accaaatcct gggcccggtg gctgacgcca atatcgaagt
     5161 ggacatgatc atccagaaca tgggtgaatc cggcaccacc gatttctcgt tcaccgtgcc
     5221 gcgcggcgaa taccaaaagg cgctgaccat cctgcgcgat gtgcaaactc acgttggcgc
     5281 cgccaaggtg gacggcgacg acaagatcgc caaggtgtcc atcatcggcg tgggcatgcg
     5341 ctcgcacacc ggcatcgcct ccaccatgtt ccgcaccctg gccgaagaag gcatcaacat
     5401 ccagatgatc tccacctcgg aaatcaaggt gtcggtgctg atcgacgaaa aatatctgga
     5461 actgggcgtg cgcgtgttgc acaaagcctt cggtctggat caggcgcagt aagattttgc
     5521 actgaactgt tgacatgaga ggtgcgtgcg cgtatagttc gcatctctcg acggagaaat
     5581 ggccgagtgg tcgaaggcac ttccctgcta aggaagcata cgggcttaaa cctgtatcga
     5641 gggttcgaat ccctctttct ccgccagaca gcgcatccgt agctcagttg gatagagtat
     5701 ctggctacga accagagggt cgggcgttcg aatcgctccg gatgcgccag caagtcccca
     5761 tcgtctagag gcctaggaca ccgccctttc acggcgataa ccggggttcg aatccccgtg
     5821 gggacgccac tgattcgaca aaaaagcctt cgctttgcga aggctttttt gcgtctgtac
     5881 tcccctgttt ttcgccccat tttctgtggt aacgccttgc ccatggcgca atccgcccca
     5941 cccgacgtca tcccaccgcg ctcactgtta aatttatatt cattaaaaac aagaccttgc
     6001 tgcaccgcaa tacaacacaa aaacccgctg aaaaccctac tgccagcatg tgcaaatcat
     6061 gacgtaagcg ttatgattga cgtttgtcga taacgtcgga gagcacgcat gagccgcttt
     6121 gattttctgc atcccccatt tgacgtgctg accgccggtg agcgcgacca ggtggaaaaa
     6181 gcggttgata tcgtgttcca ccccaacgaa gacgtcatcc tcgcccccgg ccagtcggtg
     6241 gatgccctgt atatggtgat caagggcatt gtccgcgaag tctccgacga tggcgaaatc
     6301 atcgccgtct accacagcaa cgacaccttc gacgcccgcg cgctgattgc cgggcaatcc
     6361 gtccaccgtt ttgaagtgca cgaagaagcc ctggtgttct cgctgcccaa agaactggtg
     6421 atggggctga tcgaatccaa cccggtgttt ggcgcgtttt tcttccagag cgtgtccgaa
     6481 aaattctccg ccatggccca gcgctcgggc aaccgcgagc tgcaaaccct gctcaccgcc
     6541 accgtgcgcg aagcctgtgt gaaaaccccg gtgtttctcg atggcgcaca aaccgtgctg
     6601 gacgccgccc aggcgatgaa gcgccaccgc gccaagtcca ttctggtgcg cgcgggcgaa
     6661 cgcatcggca tttttaccgc cagcgatttt cgcgacatca tcctcaccgg caccccggcc
     6721 agcgccacac tggccgacac cgcgcacttc aatctggtga ccaccgacgc gggcgactat
     6781 ctgttcaacg ccctgctgac catgacccgc cacaatatcc agcgcgtggt ggtcaccgaa
     6841 aacggccagc ccattggcgt gctggagcaa atcgacctgc tgtcgtattt ctccaaccac
     6901 tcgcacctga ttgcccagca aatcgagctg gccgactcgc tggacaccct gaagagcgtg
     6961 gccgcacaga tcgaccggct ggtgggcgtg ctgtccagcc acggcgtgaa agccccgcaa
     7021 ctggcgcggc tggtgcaaac gctgaacgcc cagctgtttg cccgcgcctg gaagctgatc
     7081 gccccggaag agctggtgga aaacagctgc ctgatcgtga tgggcagcga aggccgtggc
     7141 gaacaaatca tcaagaccga ccaggacaac gcgctgattc tgcgcgacgg cttcaaccac
     7201 ccctacctgg aagacgcctg cgcgcgcttc tcggaagcgc tgatcgaatt tggctacccg
     7261 ccctgtccgg gacaaatcat ggtcaacaac ccgctgtggc gcaaaccgca gggcgaattc
     7321 caagatcaac tctaccgctg ggtgtacctg ccggacgccg acgcgcaaat gaacctggcc
     7381 atttttgtcg atgccgaagc cgtggccggc gacgcggcgc tcttgctcca ggccaaggac
     7441 tatctgcgct cgatcatgtc cgacgatgtc gggtttttcc tgcgctttgc ccgcgccatc
     7501 gagcagttcg acaccccgct gggcctgttc tcgcagctgc tgaccaagtc gcaaggcggc
     7561 gtggccacgc tggacctgaa aaaaggcggc atcttcccca tcgtgcacgg catccgcgcc
     7621 atggcgctgg agtacaaact ggaagccagc aacacgctgg agcgcatcaa gctgctggtg
     7681 gatcagggca acctggaatc ccaactgggc cacgacaccg ccgaggcgct gagcttcttg
     7741 ctggaaatgc gcctgaaagc cgggctggat atgctggcca acggcaagac gccaagcaac
     7801 cagattcagc cagaccggct gtccacgctg gagcgcgact tgctcaagga cgcgctggcg
     7861 gtggtcaaac gcttcaagac caccattcgc caccacttca agatgaccgg gttctgatat
     7921 gctggctgcg ctgaaaaccg cctgggcgcg caagcgcctg tcctctcccg actatgccca
     7981 cctgttcgac ggccatcccg acgaaatggt cagcgtcgat tgcgaaacca ccagcctgaa
     8041 cgtgcgcgaa gccgagctat tgtcgattgg cgcggtgcgc attcgcggca accgcgtgct
     8101 gaccagcgaa tcgttttata tgctggtcaa acccgaacgc ctgcctgaag gcgccagcgt
     8161 ggcggtgcac ggtctgcgcc cgattgatgt ctccaacggc gtgccgccga tggaagcggt
     8221 gaaggcgctg ctggacttta tcggcggccg cccgctggtt ggctactatc tggaatacga
     8281 cgtcggggtg ctgaacaaat acgtcaaagg cctgctgaat atcggcctgc cgcaacagca
     8341 gatcgaagtc tccggcctgt attacgactg gaagctcaag caaaacccgg atgcttacat
     8401 cgacctgcgc ttgcagccga tgctggacga gctggaaatc cccaccctgg cgcgccacga
     8461 tgcgctgaac gacgccatca ccgcggcgat gatcctgctg gctatgcgcc agcgcggggc
     8521 gaaagtatag cccgcgcctg gcttgtattg ggattgaact gactctgcag acgccgcacc
     8581 agcgacaaca gacttgcgcc ataaaaaaac cgccgcgtca gttgacgcgg cggcgaggcg
     8641 cgggcggggt gtcagcgcaa tcaggccgac aattccagca tcagcgcgtt gatgcgttta
     8701 acaaagccgg ccgggtcgct cagttggccg ccttcggcca acagcgcctg atcaaacacg
     8761 atgtgggcca ggtcggcaaa gcgggtgtcc tggctgcgcg aggcgtcgag ctttttcacc
     8821 agcgggtgtt ccgggttgat ttccagaatc ggcttgctca ccggcacgtc ctggccggcg
     8881 gccttgagca tgcgctgcaa gtggttgctc atggcgaatt cgtccaccac caggcaggcg
     8941 ggcgattcgg tcaggcggtg ggtgacgcgc acttccttga cggtgtcttt cagcgtgtcg
     9001 ctcagcgcgt ccaccagcgg tttcatcgct tcttcgctgg ctttctggct ggctttttcg
     9061 ctttcgtctt ccagcgcgcc caggtccagc gcgccgcgcg ccaccgacac caggttcttg
     9121 ccgtcgaact cggtcaggtg cgaggtcacc cattcgtcca cgcggtcggt cagcagcagc
     9181 acttccacac ccttcttgcg gaacacttcc aggtgcgggc tggccagggc ggcggtatag
     9241 ctgtcggcgg tcaggtagta aatcttgtcc tgctccggct tcatgcgacc caggtagtct
     9301 gccaggctga cggtttgtcc atcgttgccg gtgtgggtgg aggcaaagcg cagcagtttg
     9361 gcgatgcgct cgcggttggc aaagtcctcg cccacgcctt ctttcagcac ctggccgaat
     9421 tctttccaga aggtggcgaa cttgtcggcc tggttttccg ccaggtcttc cagcatggcc
     9481 agcacttttt tcacgcagcc ggcgcggatg gcgtcgatgt ctttattgcc ttgcagcagc
     9541 tcacggctga cgttcagcgg caggtcggcc gaatcaatca ccccgcgcac aaagcgcagg
     9601 tattgcggca tcagcttgtc ggcgtcgtcc atgatgaaca cgcggcgcac atacagcttc
     9661 acgccgtggc gggcgtcgcg gtcgtacaga tcaaacggcg cgcgcgcagg caggtagagc
     9721 agctgggtgt attcctgacg gccttccacc cgcgcgtgcg tccaggccag cgggtcgctg
     9781 tagtcgtggg cgacgtgctg gtaaaacgcc ttgtattctt cgtcggtgat ttcgctcttg
     9841 gcgcgcgccc acagggccga ggccttgttc accgtctccc agccggccgg ctgggtttcc
     9901 ttgccgtctt cgtcggtgac ggcggcggtg ggcatttcga tcggcagggt gatgtggtcg
     9961 gagtacttgc ggatgatttg cttgagccgc cattcgtcca gcagctcgtc ttcgccttcg
    10021 cgcagatgca gcacgatgtg cgtgccgcgc ggggcgtcgg tggtttccag cgtgtagtcg
    10081 ccttcgccgc tggattccca gcgcacgcct tcgctggccg gcaggccggc gcggcgggtg
    10141 gtcagcgtga ctttgtcggc gacgataaag gctgaataaa agcccacgcc aaactggcca
    10201 atcaggttgg cgtccttgcg cgcgtcgccg gtgagttttt caaagaaggc gcgggtgccg
    10261 gatttggcga tggtgccgat attgtcgatc acctcatcgc ggctcatgcc gatgccgttg
    10321 tcgatcaggg tcagcgtgcg cgcctcgcgg tccacgctca ggcggatgcg cagctcgctg
    10381 tcctgctcgt agaaatccgg cttgtccagg gcttcaaagc gcagcttgtc ggcagcgtcc
    10441 gaggcattgg aaatgagttc acgcaggaaa atttccttgt ccgaatacaa ggagtgaatc
    10501 atcaactgca acagttgttt gacttctgcc tggaatccga gggtttcctt ttgagtgctc
    10561 atgatgtgct tgatgtcgtg gttgagtgaa gacctttcgc aaata
//
LOCUS       DAOPYX010000072        10600 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_7235_length_10600_cov_7.965861, whole genome shotgun sequence.
ACCESSION   DAOPYX010000072 DAOPYX010000000
VERSION     DAOPYX010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 10600)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10600)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10600
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7235_length_10600_cov_7.965861"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1215)
                     /gene="urtA"
                     /locus_tag="PKN55_05335"
     CDS             complement(<1..1215)
                     /gene="urtA"
                     /locus_tag="PKN55_05335"
                     /inference="COORDINATES: protein motif:HMM:NF024825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033219 - urea binding [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter substrate-binding protein"
                     /protein_id="HNX70694.1"
                     /translation="MLTRRFILKATALSVALAMAPTAFAADTIKVGVLHSLSGTMAIS
                     ETSLKDMALFAIDEINAKGGVLGKKLEPVVVDPASNWPLFAEKARQLLTKDKVAVTFG
                     CWTSVSRKSVLPVYEELNGLLFYPVQYEGEELSPNVFYTGAAPNQQAIPAVEYLMSKD
                     GGGAKRFFLLGTDYVYPRTTNKILRAFLKSKGVKDSDIQEVYTPFGHSDYQTIVANIK
                     KFAAGGKTAVISTINGDSNVPFYKELGNQGLKATDVPVVAFSVGEEELKGIDTKPLVG
                     HLAAWNYFMSVKNPVNSKWIADYKAWAKKKGMAGADKLVTNDPMEATYVGINMWAEAV
                     KKAGSTDVAAVSKALAGISFKAPSGFTLKMDEKNHHLHKPVMIGEVQPNGQFSVVWKT
                     AAPVRAQPWSPFI"
     gene            complement(1947..2843)
                     /locus_tag="PKN55_05340"
     CDS             complement(1947..2843)
                     /locus_tag="PKN55_05340"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HNX70695.1"
                     /translation="MTALSRIELRHLRYFLAVADTLHFGRAAERLCMSQPPLSQAIQQ
                     LEDALGVAVFERHSRKVALTPAGQALLAPARQLLADLHAAIGAAQAAARGERGLLRVA
                     YVGSAVFSVLPAIVARFHADFPEVELQLQEAPPDAQLAGLRSGQLDAGLVRPASPQAG
                     LCCDVLLDEPTWLALPSRHPFTRREALSLAELSGAPFITIDAADGGHMLELYRRADFQ
                     PRIVQHVRDLHTGFALVAAGLGLLFIPRSASRQPRPGVACRPTLDAPQLDLCLLTPDP
                     ARSPIVEHFRRVAQAVVAQMVE"
     gene            complement(3059..3793)
                     /locus_tag="PKN55_05345"
     CDS             complement(3059..3793)
                     /locus_tag="PKN55_05345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX70696.1"
                     /translation="MPTLAYTGKQSTGSKECGAYALTACLLALRKIPAQTTSLTYILP
                     TVETQSGDTMDSGKVIMRTANVPPSAVKEDLTDPCAQEIYKITGLLQDEKDVKKDVHV
                     DEQAKNSRIYPPVYSNDPDYKGLNPASALAWVAAQLGCTVAVWVDQDVRDALNQLYPR
                     EEPLLIKMRTSLPKISFVIDDIEPDLPDGAVRLVLVDDGKHWLAQDSELCYYDPATGA
                     ITEDLPAVYYDDEGNEKITGCYITLS"
     gene            4091..4933
                     /locus_tag="PKN55_05350"
     CDS             4091..4933
                     /locus_tag="PKN55_05350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011134486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HNX70697.1"
                     /translation="MTTPRAYHLVNVFAETRFGGNPLAVFEDGSGLTDAEMQAIAAQM
                     NLSETVFLFPPRLPGCDAHVRIFTPGYELPFAGHPTLGSAAMVRRLGRGGDHVQLGMA
                     AGVFPVRAEGERWQLAAKSPVAQPWVTHTHHLAGALGLSPNEVRAARLIDCGTEQLLV
                     EVCCREAVLNCRPDARLLEAYCRNRQGVAMAYVWHIEQGVATVRLFFTHHGQVVEDPG
                     TGSACANLGGWLQLAGKHPLTLRVEQGHALKRPNVLHLAVDAAGEITVAGEVIWLGQG
                     VLHV"
     gene            complement(5202..6665)
                     /locus_tag="PKN55_05355"
     CDS             complement(5202..6665)
                     /locus_tag="PKN55_05355"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HNX70698.1"
                     /translation="MRRAAELELALSPRPAGQTLQHWLYQELRAALLSGRLPAGARLP
                     PSRELARQQGVSRSTVLAVYEQLSAEGYLHGAVGRGTTVATRLPAPQPTPRVPASTAL
                     RLSHAGQRLADSPFPREAGAPLRAFRANHPDLNAFPLAVWNGIVARREQALRPSSLGY
                     ADPMGEPALRQAIAEHLRYAQKIVCQPEQILIVASAQQGLDICARLLLDPGDAVWMED
                     PGHPGARDVFAATGAQVMAAPVDAHGLDVAAAQALAPHARLAYVTAAHQAPLGMALSL
                     ERRLALLAWAERANAVVIEDDYDGEYRYSGPPLAALKSLDQQGRVIYLGSFSKLLFPA
                     LRLAYLVLPDALVDAFAAALALTCRHVATPSQRVLAAFIAEGHFARHLRRMRLLYAER
                     ADCLQQACRRELAGVLDLPPINTGLDAAAWLPAHWDDRAVSRQLAAAGIEARPLSFYR
                     IARQPPPGLLLGFAALNPAQIETGVAEMVQVLGTLSA"
     gene            6787..7224
                     /locus_tag="PKN55_05360"
     CDS             6787..7224
                     /locus_tag="PKN55_05360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011135071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HNX70699.1"
                     /translation="MQARFDVGAVAPEGFQAMYGVHQYVDHSGLPLRLLELLRLRVSQ
                     LNGCAFCLAMHTPLARRHGVTEAELTLLPAWREAPTLSAAERATLAWAEALTQLTPLG
                     VDDALYAEMQVHFDSKQIADLSFAVAEINAWNRLMVASRTPPQ"
     gene            7453..8706
                     /locus_tag="PKN55_05365"
     CDS             7453..8706
                     /locus_tag="PKN55_05365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014087227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HNX70700.1"
                     /translation="MRANACGVDFGTSNSTVGWLRPDADVLLALEDSKATLPSVVFFN
                     AEEDHTVFGRQALAEYLDGYEGRLMRSLKSILGTGLVNGQTEVNGQALRFIDLLSLFI
                     RTLKARAETSAGREFSQVVFGRPVFFVDDDAQADQLAQDTLADIARRAGFSEIEFQFE
                     PIAAAFDYESQIEREALVLIVDIGGGTSDFSLLRLSPERRGQLDRASDILASGGVHIG
                     GTDFDRQLSLQSVMPLLGYRSLLQNGVEVPSSYYFNLATWHTINFAYTRQVQAELQAV
                     RREAGERHKLDRLLALIDQRAGHWLAIQVEAAKIALSSTEQHTMDLAQLEASLSCLVA
                     REEFDQAIDSLLARIETTLSRLLADAQLRAAQVDTVFFTGGASGVPLLRQRVAAMFPA
                     ARVVEGDRDGSIGRGLARDAACRFA"
     gene            8896..9993
                     /gene="aroC"
                     /locus_tag="PKN55_05370"
     CDS             8896..9993
                     /gene="aroC"
                     /locus_tag="PKN55_05370"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012697987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HNX70701.1"
                     /translation="MSGNTFGLLFTVTSFGESHGPAIGCVVDGCPPGMALDAAWIQAE
                     LDRRKPGTSRHVTQRREPDEVEILSGVFEGKTTGTPIALLIRNTDQRSKDYGNIADTF
                     RPGHADYTYWHKYGIRDPRGGGRSSARETAVRVAAGAIAKKWLAEQFGIVIRGHMTQI
                     GEKIIPFASWDEVNSNPFFAADASVVPELEAYMDSIRKSLDSIGARLRVVAENVPVGW
                     GEPVFDRLDADIAHAMMSINAVKGVEIGAGFGCVTQKGSEHGDELTPQGFASNHAGGI
                     LGGISTGQDIEVSIAIKPTSSIAQPRRSINKAGEAIEMETHGRHDPCVGIRATPIAEA
                     MLALTLIDHALRHRAQCGDVRVDTPDIARRG"
     gene            complement(10080..>10600)
                     /locus_tag="PKN55_05375"
     CDS             complement(10080..>10600)
                     /locus_tag="PKN55_05375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX70702.1"
                     /translation="GRGGNDIINGYGGNDTLQGGDGNDYLYVATTVDGGLGITSLSGG
                     SGNDVFYMGRYLVSNPNLNMSNVTITDFVSGQDKIRFDIAQSLGVAAPNALIKLVAGS
                     GETLADLLDRAGNQASGSRAALVSFQLDGNTYVVLDTHPASDVFGDTDTGITLTGLVD
                     VQWSDFACVVVV"
BASE COUNT         1940 a         3288 c         3413 g         1959 t
ORIGIN      
        1 gatgaacggg ctccacggtt gcgcgcgcac cggggcagcg gtcttccaca ccaccgagaa
       61 ctggccattc ggctgcactt cgccaatcat caccggcttg tgcaggtggt gattcttttc
      121 gtccatcttc agggtgaagc cagacggcgc cttgaagctg atgccggcca gcgccttgct
      181 caccgccgcc acgtcggtgc tgccggcttt cttgaccgct tcggcccaca tattgatgcc
      241 cacataggtg gcttccatcg ggtcgttggt caccagcttg tcggcgccgg ccatgccctt
      301 cttcttggcc caggctttgt aatcggcaat ccacttgctg ttgaccgggt tcttcacgct
      361 catgaagtag ttccaggcgg ccagatggcc caccagcggc ttggtgtcga tgcccttgag
      421 ctcttcctca cccaccgaga acgccaccac cggcacatcg gtggccttca ggccctggtt
      481 gcccagctct ttgtagaacg gcacattgga atcgccgttg atggtggaaa tcaccgccgt
      541 cttgccgcca gcggcaaatt tcttgatgtt agcgacgatg gtctggtaat cgctgtggcc
      601 aaacggggtg tacacctctt ggatatcgct atccttcacg cccttggatt tcaggaaggc
      661 gcgcagaatc ttgttggtgg tgcgcgggta cacatagtcg gtgcccagca ggaagaaacg
      721 cttggccccg ccgccgtcct tgctcatcag gtattccacc gccggaatcg cctgctggtt
      781 cggcgcggcg ccggtgtaga acacattggg cgacagctct tcgccttcat actgcaccgg
      841 gtagaacagc aggccattca gctcttcgta caccggcagc accgacttgc gcgacaccga
      901 cgtccaacag ccaaacgtga cggccacttt gtccttggtc agcaactgac gggccttctc
      961 ggcaaacagc ggccagttgg acgccggatc caccaccacc ggctccagct tcttgcccag
     1021 cacgccaccc ttggcgttga tctcgtcaat ggcgaacaag gccatgtctt tcagcgaggt
     1081 ttcggaaatc gccatggtgc cggacagcga atgcagcaca cccaccttga tggtgtcagc
     1141 ggcaaaagca gtcggggcca tcgccagggc gacagaaagg gcagtggctt tcaggatgaa
     1201 acgacgggtc agcatggggg gcgctcctgt gttggacttt acggtgtctg gggtgctgca
     1261 acatgggagg caatgtacgg cgggagtgct gcactgcgga atacgtcatc ttgcgtatgg
     1321 gggtgggggc acaggaaaaa acggtgagtc aggcgagtgt cgtggtgcag tgccacacat
     1381 atgaaaacgg cgcgcaagcc aaagctgcgc gccgtgatca gggattaaga attgctcagt
     1441 cagggcatca tgcccaccgc tcaaaaatag cgtttattgc cgccactggt gatgcaatac
     1501 accccgccac gcggcccggt gcaggtggcg ccagagccgc agtcacaacg gctgccatag
     1561 ccactatggt cagcctgggc cgggcgatgg gacgcccggg catgccggcg cgtgctgtgg
     1621 ccatgggact tggccacggc atggtgacgg ctatagtgat gtccgccgcc accgccgtgg
     1681 cgtccgccgc gcgcttcggc aagagtgggt aaagccagcg caatggccag cacacagagg
     1741 gtaaatatac gcatgatgaa tctccggaag ggtcagttgg atgacagcat tagaaatatg
     1801 ctttatgaga attttgtggt gattataccc caaagactct cttgttttct gtcaaatcaa
     1861 cttggcgcag caatgcggga cattcagcgc actaaaggcc aaattaccgg ggccacatgc
     1921 atggtcatgt gggcgcagac ggcaactcac tccaccatct gcgccaccac cgcctgcgcc
     1981 acgcggcgaa aatgttcgac aatcggcgag cgggccgggt ctggcgtcaa caggcacaga
     2041 tcaagttgcg gggcgtccag ggtggggcga caagcaacgc cggggcgggg ttggcggctg
     2101 gcggagcggg ggatgaacaa gaggcccagg ccggcggcca ccagggcaaa gccggtgtgc
     2161 aggtcgcgca cgtgctggac aatgcgcggc tgaaaatcgg cgcggcggta cagctccagc
     2221 atatggccgc cgtcggcggc gtcgatggtg atgaacggcg cgccagagag ctcggccagg
     2281 ctgagcgctt cgcgccgggt gaagggatgg cggctgggca gcgccagcca ggtgggttca
     2341 tccagcagca catcgcagca cagcccggct tgcgggctgg ccggacgcac caggccggcg
     2401 tccagctggc cgctgcgcag gccggccagt tgcgcgtcgg gcggggcttc ttgcagttgc
     2461 agctccacct cgggaaagtc ggcgtgaaag cgggcgacga tggccggcag caccgaaaac
     2521 accgccgagc cgacataggc cacccgcaac agcccgcgct cgccgcgcgc cgccgcttgg
     2581 gcggcgccga tggcggcgtg caggtcggcc agcagctgac gggccggggc cagcagcgcc
     2641 tggccagccg gggtcagcgc cactttgcgg ctgtggcgct cgaacaccgc cacgcccagt
     2701 gcgtcttcca gttgttgaat ggcctgcgac agcggcggct gcgacatgca cagccgctcg
     2761 gcggcgcggc caaagtgcag ggtgtcggcc accgccagaa aatagcgcag gtggcgaagt
     2821 tcaatgcggg acagggcggt catggcggcg caggcgcgtg gcggaagcgc catgatagca
     2881 agacgccgcc cgctctatcg gcctgacgcg ctgtgcggcg cacaaaactc agcgtggtgt
     2941 gccggtcagg caggtggcgg acggctgggc aggatggcca gatggtgctg gggtaatccg
     3001 cactgtctgc ggccgcgccc ctggccagga acgctttgct gcgttttctt ggcgatgctt
     3061 agctcagggt aatgtaacaa ccagtgatct tttcgttacc ctcatcatca taatagacag
     3121 caggaagatc ttccgtgatg gcgccagtgg ctgggtcgta gtagcacagc tcactgtcct
     3181 gggcaagcca gtgttttcca tcgtcaacca gcaccagtcg tacggcgcca tctggcaagt
     3241 ccggttcgat gtcgtcgatg acaaacgaaa tcttgggcaa ggaggtcctc atcttgatga
     3301 ggaggggttc ttcgcgggga tataattgat tcaaagcatc tcgcacatcc tgatctaccc
     3361 atacagccac ggtgcagccc aattgcgcgg caacccaggc cagggcggag gccggattca
     3421 atcccttgta atccggatcg ttgctgtaaa cgggcggata gattcgagaa ttcttggcct
     3481 gttcgtctac gtgtacgtct ttttttacgt ctttttcgtc ttgtaatagc ccggtgatct
     3541 tatagatctc ctgtgcacac gggtcggtca agtcctcttt gacggcgctg ggtggcacat
     3601 tggctgtgcg cattatcact ttgccggaat ccatcgtgtc gccagactgc gtttctacgg
     3661 ttggcaagat gtaggtcaac gatgtggttt gagccgggat tttgcgcagt gccagcaagc
     3721 atgcggtcag cgcgtatgcg ccacattcct tactgcctgt agattgtttg ccggtataag
     3781 cgagtgtagg catggatgac aggctcctgt aaaaagacga ctaaaaagac gacaaaatcg
     3841 gacacgcaac cggtggctaa gagctgcgca taaaactcag cgcagcgatc ctggcctggc
     3901 gcgcggccgc agacaaagca gtatgacaag gccgagcaac gacgccagaa gggtttggtt
     3961 ggccgctaag cccctgcaca cacaggacgc agttgtgctg cggctgtgaa gtcaggcagg
     4021 tctgtgcatt gcgccgattt cctttacaat tttcagcctg acttttcctg acttgtgagc
     4081 cctgaccgcc atgaccacgc cccgtgctta tcatctggtg aatgtatttg ccgaaacccg
     4141 gtttggtggc aacccgctgg cggtgttcga agatggctcc gggctgacgg atgccgagat
     4201 gcaggcgatt gccgcgcaga tgaatctgtc cgaaacggtg tttctgtttc cgccacgcct
     4261 gccgggctgt gatgcccatg tgcgcatttt cacccccggc tatgagctgc cgtttgccgg
     4321 ccatcccacc ttgggcagtg cggccatggt gcgccggctg gggcgtggtg gcgatcatgt
     4381 gcagctgggc atggccgccg gggtgtttcc ggttcgtgcc gaaggcgagc gctggcaact
     4441 ggccgccaag tcgccagtgg cgcagccctg ggtgacccac acccaccatc tggcgggtgc
     4501 gctgggcctg tcgcccaacg aagtgcgtgc cgcccggctg attgactgcg gcaccgagca
     4561 attgctggta gaagtctgtt gccgtgaggc cgtattgaac tgtcgcccgg acgcccgcct
     4621 gctggaagcg tattgtcgca atcgtcaggg cgtggccatg gcctacgtct ggcatatcga
     4681 gcagggcgtg gccaccgtac gcttgttttt tacccaccat ggccaagtgg tggaagaccc
     4741 tggcactggc tcggcctgcg ccaatctggg cggctggctg caactggccg gcaagcaccc
     4801 gctgactttg cgggtggagc agggtcacgc gctgaagcgg ccgaacgtgc tgcatctggc
     4861 ggtggatgcc gccggggaaa tcaccgtggc cggcgaggtg atctggctgg ggcagggggt
     4921 gttgcatgtg tgagcgcgca tgggcggcgc cacgcggctg ataacacacc tggtgcaggc
     4981 gcacgccaca gaagggtggc aaggtgatgt cgcgctgcta cccagcgcaa cgcgcctggt
     5041 caggtgcgcg accgcggaca ggacgagagt gccacaacgc caggcgctta gagtcgctaa
     5101 caaaacccag cgaagcggtc ctggctaggc gtgtgaccgc agacagtaca agtagtacga
     5161 caaggtcaca caacaacgcc agggggtttt gttagcggct cttaagcgga caaggtgccc
     5221 agcacctgca ccatttcggc cacgccggtt tcgatctgcg ccgggttgag cgcggcaaag
     5281 cccagcagca ggccgggcgg cggctggcgg gctattcgat aaaacgacaa gggccgcgct
     5341 tcgatgccag ccgctgccag ttggcgggag accgcccggt catcccagtg ggccggcagc
     5401 caggcggcgg catccagccc ggtgttgatg ggcggcagat ccagcacgcc ggccagctcg
     5461 cgccggcatg cctgttgcag gcagtcggcg cgctcggcgt acaacaggcg catgcggcgc
     5521 aggtggcggg caaagtggcc ttcggcaata aacgccgcca gcacccgctg tgatggggtg
     5581 gccacatgcc ggcaagtcag cgccagcgcg gcggcaaagg cgtccaccag tgcatccggc
     5641 agcaccagat acgccagccg caaggccgga aacaacagct tgctgaacga gcccagataa
     5701 atcacccgcc cctgctgatc caggcttttc agcgccgcca gcggcgggcc gctgtagcgg
     5761 tattccccat cgtaatcgtc ttcaatcacc accgcattgg cgcgttcggc ccacgccagc
     5821 aaggccagcc gccgctcaag gctcaacgcc atgcccagcg gcgcctgatg ggcagcggtg
     5881 acgtaggcca accgggcatg cggcgccagt gcctgcgcgg ccgccacgtc caggccatgc
     5941 gcatccaccg gcgcggccat cacttgcgcg ccagtggcgg caaacacatc acgtgcgccg
     6001 gggtggccgg ggtcttccat ccatactgcg tcgccagggt ccagcaacag ccgcgcgcag
     6061 atatccagcc cttgttgggc gctggcgaca atcaggatct gttccggctg gcagacgatt
     6121 ttttgtgcgt agcgcagatg ctcggcaatc gcctgacgca gggccggctc gcccatcggg
     6181 tcggcgtagc ccaggctgct cgggcgcagg gcttgttcgc ggcgggcgac aatgccattc
     6241 cataccgcca gcggaaaggc gttcaggtcg gggtggttgg cgcgaaaggc gcgcaagggc
     6301 gcgccggctt cgcgcggaaa tggcgagtcg gccaggcgct ggcccgcgtg cgacaagcgc
     6361 agcgccgtgc tggctggcac gcgcggggtg ggctgcggcg ccggcaggcg ggtggccact
     6421 gtggtgccac gccctaccgc gccatgcaga tagccttcgg cgctcagttg ctcgtacacc
     6481 gccagcaccg tgctgcgcga cacgccctgc tggcgggcca gttcgcggct ggggggtagg
     6541 cgtgcgccgg cgggcaaacg gccactgagc aaggctgcgc gcagctcttg atacaaccag
     6601 tgttgcaggg tctggccggc cgggcgcggg ctcagggcaa gttcaagttc ggcggcgcgg
     6661 cgcattggtg gggctcacag gcaaattggc ctgatgattt tgccgctaaa tggctatgtt
     6721 tgtcaggcca atttgccgct aagctggccg gtgtgttgat cccatccccc gatcaggaga
     6781 atgaccatgc aagcccgttt tgatgttggc gcggtggcgc cggaaggttt tcaggcgatg
     6841 tacggcgtgc accagtatgt ggaccacagt ggcttgccgc tgcggctgct ggagctgttg
     6901 cgcctgcgcg tgtcgcagct gaatggctgc gccttttgcc tggccatgca cacgccgctg
     6961 gcgcgccgcc acggcgtgac tgaggctgaa ctcaccctgt tgccggcctg gcgcgaagcc
     7021 ccgacattgt ctgctgccga gcgcgccacg ctggcctggg ccgaggcgtt gacccagctc
     7081 accccactgg gcgtggacga cgcgctgtat gccgagatgc aggtgcattt tgacagcaag
     7141 cagattgccg acctcagctt tgccgtggcg gaaatcaacg cctggaatcg gctgatggtg
     7201 gcctcgcgca cgccgccgca gtaagcgccg ctcaccaaac cctcctggcg ttgtgaccga
     7261 tatggtctgc ggtcacacgc ctggccatca tcgctgcgcg gggttttgtg cgcggcgctt
     7321 ggccaggggg taaggtgaca tgatgtgaag gcccgcagcc cattccctca ccgcgaaacc
     7381 tgacatgtaa ttgtcccgga aaaccttgca catcaatgct ataatcgccc gcttttttct
     7441 gcggagcctg cgatgcgtgc gaatgcctgt ggcgttgact ttggcacttc caattccacc
     7501 gtgggctggt tgcgcccgga cgccgacgtc ttgctggcgc tggaggacag caaggccacg
     7561 ctgccctcgg tggtgttttt caacgccgaa gaagaccaca ccgtgtttgg ccgccaggcg
     7621 ctggccgagt atctggatgg ctacgaaggc cggctgatgc gctcgctgaa aagcattctg
     7681 ggcaccggcc tggtcaatgg ccagaccgaa gtcaatggcc aggcgctgcg tttcatcgac
     7741 ctgctctctt tatttatccg caccctgaaa gcccgcgccg aaaccagcgc cgggcgcgag
     7801 ttcagccagg tggtgtttgg ccggccggtg ttttttgtcg atgacgacgc ccaggccgac
     7861 caactggcac aggacaccct ggctgacatc gcccgccgcg ccgggtttag cgagattgaa
     7921 ttccagtttg aaccgattgc cgccgcgttt gactacgaaa gccagatcga gcgcgaggcg
     7981 ctggtgttga ttgtcgatat cggcggtggc acctcggact tttcgctgct gcgcctctcg
     8041 cccgagcgac gcggccagct ggatcgcgcc agtgacatcc tggccagcgg cggcgtgcac
     8101 atcggcggca cggactttga ccgccagctc agcctgcaat cggtgatgcc gctgctgggc
     8161 tatcgctcgc tgctgcaaaa cggcgtggaa gtgccgtcca gctattattt caacctggcc
     8221 acctggcaca ccatcaactt tgcctacacc cgacaagtgc aggccgaatt gcaggcggtg
     8281 cgccgcgagg ccggcgagcg ccacaagctg gaccggctgc tggccttgat tgaccagcgc
     8341 gccggccact ggctggccat tcaagtcgaa gccgccaaga ttgccctgtc cagcaccgag
     8401 caacacacca tggacctggc ccagctggaa gccagcctga gctgcttggt ggcgcgtgag
     8461 gaattcgacc aggccatcga cagcttgctg gcacgcatcg aaaccaccct gagccgcctg
     8521 ctggccgacg cacaactgcg tgctgcccag gtagacacgg tgttttttac cggcggcgcc
     8581 agcggcgtgc ccttgctgcg ccagcgcgtg gcggcgatgt tccccgctgc gcgcgtggtg
     8641 gaaggcgacc gcgacggcag cattggccgt ggcctggcgc gcgacgccgc ctgccgcttt
     8701 gcttagcacc gcgccgtcaa cactgtaagg atcgtcacac cctgcgtagc caggaagcta
     8761 gcgtgtggat tttgccgtgc ttggcggtgt atcacactgg ccgattgagc ctcagaccgc
     8821 ccgcgccaaa agcgggctgc cgccttgtga tacactgaac gccgttttta accctcccgc
     8881 ctgcacgagc ccaccatgtc tggaaatacc ttcggcctgt tgttcaccgt cacctccttt
     8941 ggcgaaagcc acggcccggc cattggctgc gtggtggatg gttgcccgcc gggcatggcg
     9001 ctggacgccg cgtggattca ggccgaactg gaccgccgca agcccggcac cagccgtcat
     9061 gtcacccaac gccgcgagcc ggatgaggtg gagattctgt ccggtgtgtt tgagggcaag
     9121 accaccggca cgccgattgc gctgttgatc cgcaacaccg accagcgcag caaggactac
     9181 ggcaatatcg ccgacacctt ccgccccggc catgccgatt acacctactg gcacaaatac
     9241 ggcatccgcg acccgcgcgg cggcgggcgc tcgtccgcac gggaaaccgc cgtgcgcgtg
     9301 gcggccgggg cgattgccaa aaaatggctg gccgagcaat tcggcattgt catccgtggc
     9361 catatgaccc agatcggcga aaaaatcatt ccgtttgcca gctgggacga agtgaacagc
     9421 aacccgtttt ttgccgccga cgccagcgtg gtgcccgagc tggaagccta tatggacagc
     9481 attcgcaaaa gcctggattc gattggcgcg cggctgcgcg tggtggccga aaatgtgccg
     9541 gtgggctggg gcgaaccggt gtttgaccgc ctggacgccg acattgccca cgccatgatg
     9601 agcatcaacg cagtgaaagg cgtggaaatc ggcgccggtt ttggctgtgt cacgcaaaaa
     9661 ggcagcgagc acggcgacga gctcaccccg caaggttttg ccagcaacca cgctggcggc
     9721 atcctgggcg ggatttccac cggccaggac atcgaagtct cgattgccat caagcccact
     9781 tcgtccatcg cccagccgcg ccgctcgatc aacaaggccg gcgaagccat cgaaatggaa
     9841 acccacggcc gccacgaccc ctgcgtgggc atccgcgcca cgccgattgc cgaagccatg
     9901 ctggcgctga cgctgatcga ccacgcgctg cgccaccgcg cccagtgcgg cgatgtgcgg
     9961 gtggatacgc ccgatattgc ccgacgcggt tgagcccggc ggtttgccgg caggcgataa
    10021 aaacccccgg ccttggccgg gggtttttat cgacgctcgt caatgcagcc tgatttctat
    10081 caaaccacca ccacgcaggc aaaatccgac cattgcacat ccaccaggcc cgtcagggtg
    10141 atgccggtat cggtatcgcc aaatacatcg ctcgccgggt gggtgtccag caccacatag
    10201 gtgttgccat caagctgaaa agacaccagc gctgcccgac tgccgctggc ctggttgccc
    10261 gcccggtcca gcaggtcggc caaggtttcg cctgagcctg caaccagctt gatcagtgcg
    10321 ttgggggcgg cgacgcccag gctctgggcg atgtcaaaac ggattttgtc ctggccgctg
    10381 acaaaatcgg tgatggtgac attggacata ttcagattgg gattgctgac caggtagcgc
    10441 cccatgtaaa acacatcatt gccgctcccc ccgctcaggc tggtgatgcc cagcccgcca
    10501 tccaccgtgg tggccacata cagatagtcg ttgccgtcgc ctccctgtag tgtgtcattg
    10561 ccgccatagc cattgatgat gtcgttaccg ccccgaccat
//
LOCUS       DAOPYX010000073        10590 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_7250_length_10590_cov_8.922449, whole genome shotgun sequence.
ACCESSION   DAOPYX010000073 DAOPYX010000000
VERSION     DAOPYX010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 10590)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10590)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10590
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7250_length_10590_cov_8.922449"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1097)
                     /locus_tag="PKN55_05380"
     CDS             complement(<1..1097)
                     /locus_tag="PKN55_05380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014087043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNX70703.1"
                     /translation="MKILSLRLKNLNSLKGEFCIDFRQPPFAGNGLFAITGPTGAGKS
                     TLLDAICLALYHRTPRLEAVSAGGNELMTRHTADCLAEVEFEVRGVGYRAFWSQRRAR
                     DKASGALQAPKVELARADGEILASQVNDKLRQVEALTGLDFARFTKSILLAQGGFAAF
                     LHASANERAELLEQLTGSEVYGLISQRVFDHARAARQALAEQHARADGVQLLSAEARA
                     ELLADCAARRQALDALTEQLAHTQACLNWRRALDAAETEQHKQHAALARCQAEREHAA
                     PDLARLAASEPAAAIAPAHHDWRQAIARHDAAAAALSQLEAERRNLATHHARSHWQAA
                     HYARALTERAVARQAELDTQLADANAWLASHR"
     gene            complement(1097..2314)
                     /gene="sbcD"
                     /locus_tag="PKN55_05385"
     CDS             complement(1097..2314)
                     /gene="sbcD"
                     /locus_tag="PKN55_05385"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008954070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HNX70704.1"
                     /translation="MRILHTSDWHLGQHFIGKTRQAEHRALIAWLLEQVSALNIDAVL
                     LAGDVFDTGAPPSYARELYHQLLAGVAAAGASLLVVGGNHDSAATLGEIRPLLGALRA
                     TVIPSADLPLDEQVVLLPRRDGQPGALVCAAPFIRPRDVLRSQAGQDADDKQGALQAA
                     ITQHYHALYQRALARRAALGLALPIIATGHLTTVGASASESVREIYVGALEAFPTQAF
                     PPVDYLALGHIHRPQCVGGLNHIRYSGSPLALSFDEADTHKQMWLLDFAEGQLSEVSA
                     LPVPCAQALASLSGDLASLPAQLAAVAAQGSAERPVWLEVRVRGDDYLSDLAARVAAL
                     ADGLPVEILRVRRERGDAVASLSADPGDTLDALSPLEVFERRLAQETLTPEQHDGLLS
                     RYRQVLAELAETA"
     gene            2525..3634
                     /locus_tag="PKN55_05390"
     CDS             2525..3634
                     /locus_tag="PKN55_05390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012697593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HNX70705.1"
                     /translation="MPVPLPLLLPDRPAASPALAAPQLIAAQSDAEAVITWLREYEDS
                     PHTFANYRKEAERLLLWCGERGQTLAGLGREDILDYQRFLASPQPAERWIGPARARQH
                     PDWKPFTGPLAPSSIRQALTILGALFQYLLDAGYLRGNPLALARRQKRSLAQPPGVER
                     YLDAELWQVVQDTLEALPRSGARDLHHYERARWLFHLLYLTGARRTEVARAVMGDIVQ
                     RRGLWWWRVLGKGNKIGDIPVGDSLLAALQRYRSHCGLPPLPLPGEATPLVGRVNQPG
                     KLDTLSDKAIYLIVKQVFRLAAEREPAQAERLAAASTHWLRHTAASHQLEAGVPLLVV
                     SQNLRHASIQTTRKYLHTEDDARHQASQAHGLGEI"
     gene            complement(3736..4407)
                     /locus_tag="PKN55_05395"
     CDS             complement(3736..4407)
                     /locus_tag="PKN55_05395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX70706.1"
                     /translation="MSYLRVLATAALALTLTAPAQAEGGTVTIEKDRFIDFYLVLKDH
                     LSRMPGLRGDGVTLSRAYLLDKLRERFNKEGVEVRDLRLTPEKGTIELVTTKGPVDAR
                     HTVDFTFLPVDWAQRTIRLRFQQRSEPLNDTLLGQLLGSIAIGAFETATGRNGVKQLT
                     EKHPYLTVDGDVLSVRMDQVPSLIPYLNTQIMGYKPFDYVGVKRVATEQDQLRIVLAL
                     QKKPS"
     gene            complement(4425..4952)
                     /locus_tag="PKN55_05400"
     CDS             complement(4425..4952)
                     /locus_tag="PKN55_05400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014087672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HNX70707.1"
                     /translation="MAALLAVALAACSSSSPPRKTQTASKPKPPARTASAKPNANRSL
                     ANIQASGDAREVVMYALGLLDVGYKFGGSNPEAGLDCSGMAAFIYKNAVNVQLPHNAK
                     QIAEATKPIDTGKMRAGDMVFFNTMNRPYSHMGIYIGDGKFIHAPRTNSVIRVDTLAS
                     GYFADRLEGARTVFD"
     gene            complement(4961..6883)
                     /locus_tag="PKN55_05405"
     CDS             complement(4961..6883)
                     /locus_tag="PKN55_05405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012697587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNX70708.1"
                     /translation="MPLITVEKACLAFGHVPLLDHVDFSLEPGERVGLIGRNGAGKSS
                     LLKAIAGTAKLDDGRIIAQSDTRVAYVPQEPALDPAHTVFEAVAEGLGDLRQLLTDYH
                     HAVQALSTPSDDGHEAVLAAMQEIQNQLEARQGWQLENLIDSTLSHLGLPADRLIGEL
                     SGGWKKRVALAQALVCSPDVLLLDEPTNHLDIAAIEWLETLLKNFAGSVLLITHDRRF
                     LDNVSTRIIELDRGLLRSYPGSFSAYQTRKAEELAVEAEQNRKFDKFLAQEEVWIRKG
                     VEARRTRNEGRVLRLEQLRRDRAARRERVGQVNFRLDPGERSGKLVAELEHVSKGYEG
                     RTLIRDFTSRLLRGDRIGLIGPNGAGKTTLLKLILGEIQPDSGTVKPGSKLEIAYFDQ
                     FRSQLDDEMAIVDVIGDGKDFVEIGGQRRHVMSYLEDFLFSPARAKSPVKSLSGGERN
                     RLLLAQLFTRPANVLVLDEPTNDLDIDTLELLEQLLADYSGTVFLVSHDRAFLDNVAT
                     QVIAFEGDGVLMEYPGGYSDWLAAKARMAELAAPARTVAVAPVKTDKAEASAKPARRS
                     NKLSYNETRELERLPDDIAELEAEQAALQEALLNPDIYKTDPKQAHEWQLRVNEIDTL
                     LLDKLARWDELESRAS"
     gene            complement(7068..8300)
                     /locus_tag="PKN55_05410"
     CDS             complement(7068..8300)
                     /locus_tag="PKN55_05410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018152756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HNX70709.1"
                     /translation="MNVDFFLPWFTRLRHAASAGLLIALVALLAGSASALFLAALDWA
                     TATRLAHPWLLAGLPLAGFAVGWVYWRVGQPVEAGNNLLIDEIHDPKNTIPLRMAPLI
                     LAATVISHLFGASVGREGTAVQMGGALADQLNRLWPQDAAGRQRVLMTGISAGFASVF
                     GTPLAGAVFALEVLAIGRLRVDALLPCLLAAMLADQVCLAWGAQHAHRALGALPALSA
                     SSLIAVLAAGALFGLAGRSFAWATHSLGRQFKTRISYPPLRPLLGGALFAVTVWALGG
                     ERFQGLGVNLIDAAFVGQLGPGEALGKWAATVWSLACGFKGGEVTPLFAIGATLGNAL
                     AALLPLPAPLLAALGLAAVFAGAANTPLACTLLAIELFGGGIGLFAALACAASYACSG
                     HQGIYRAQRLEAPKHRPA"
     gene            8480..9433
                     /gene="thiL"
                     /locus_tag="PKN55_05415"
     CDS             8480..9433
                     /gene="thiL"
                     /locus_tag="PKN55_05415"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014087455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HNX70710.1"
                     /translation="MNEFDLIRRYFDRPAADPAVRLAVGDDAAILAPTPGHELLVSTD
                     MLVCGRHFFADVDPRALGHKALAVNLSDIAAMGGTARWATLAAAWPELDADWLAAFAE
                     GWFALADAHEVALIGGDTTRGPLTLSVSIFGEAPAGRALRRDGGRAGDDVWVSGELGM
                     AAAAVRARLGQLTLPEAERALAQQRLDWPQPRLALGQALRGLASAALDVSDGLLADLG
                     HLAARSGVAAWVRADWLPMPACLREQGAAGLACAAAGGDDYELCFAAPPSARAALADL
                     GRALAVPLTRIGALTEGVGCRLLDADGAEILLEQHGYDHFA"
     gene            9417..9911
                     /locus_tag="PKN55_05420"
     CDS             9417..9911
                     /locus_tag="PKN55_05420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018151678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HNX70711.1"
                     /translation="MTISPDWRFMLRHPAHVIALGLGSGLAAKAPGTFGTLAALPLYA
                     LWLALGLSPTLLAWLLPLLFALGCWACQRTGDALGAPDHGAMVWDEMVAMWALLIVLP
                     ATPLAWALGFALFRVFDIVKPWPIRWLDAQLKGGFGVMLDDALAALMAWAVWRLAAPA
                     LGLG"
     gene            10145..10540
                     /locus_tag="PKN55_05425"
     CDS             10145..10540
                     /locus_tag="PKN55_05425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX70712.1"
                     /translation="MSVSLDTPEDIANWLRRHFVGQTFGCVRFWQFALVRPNDQFFQL
                     ASVTLAGDRLDLLLRHADGQGEAGVVSVWQPMGLSPRPHGVALSAAARLSWGNSEAWQ
                     AGEGQYRIRTPRGEGGFAIEGAPALTLEC"
BASE COUNT         1688 a         3413 c         3636 g         1853 t
ORIGIN      
        1 cggtggctgg ccagccaggc gttggcgtca gccagttgcg tgtccagctc ggcttggcgg
       61 gcgacggcgc gttcggtcag cgcgcgggcg tagtgggcgg cttgccagtg gctgcgggcg
      121 tgatgagtgg ccagattgcg ccgttcggcc tccagctggc tcagcgcggc ggcggcggcg
      181 tcgtggcggg cgatggcctg gcgccagtcg tgatgggcgg gggcgatggc ggcggcgggc
      241 tcgctggcgg ccagccgcgc caggtcgggg gcggcgtgtt cgcgctcggc ctggcagcgc
      301 gccagcgcgg cgtgctgctt gtgttgttcg gtttcggcgg cgtctagcgc gcgccgccag
      361 ttcaggcagg cttgggtgtg cgccagttgt tcggtcagcg cgtccagcgc ctggcggcgg
      421 gcggcgcagt cggccagcag ctcggcgcgc gcctcggcgc ttaacagctg cacgccgtcg
      481 gcgcgcgcgt gttgttcggc cagcgcctgg cgggcggcgc gggcgtggtc aaacacccgt
      541 tgtgagatca ggccatacac ttcgctgccg gtcagttgct ccagcagttc ggcgcgttcg
      601 ttggcgctgg cgtgcaaaaa ggcggcaaaa ccgccctgcg ccagcaggat ggatttggtg
      661 aagcgggcaa agtccaggcc ggtcagcgcc tcgacctggc gcagtttgtc attaacctgg
      721 ctggcgagga tttcgccatc ggcgcgggcc agctccactt tgggcgcttg cagcgcgccg
      781 ctggctttgt cgcgggcgcg gcgctggctc cagaacgccc gatagccgac gccgcgcact
      841 tcaaactcca cttcggccag gcagtcggcg gtgtggcggg tcatcagctc gttgccgccg
      901 gccgacaccg cctccagccg gggggtgcgg tggtagagcg ccaggcagat ggcgtccagc
      961 agggtggatt tgccggcgcc ggtggggccg gtgatggcaa acaggccatt gccggcaaac
     1021 ggcggctggc gaaaatcaat gcaaaattcg cccttgagcg agttcaggtt tttcaggcgc
     1081 agcgagagga ttttcatcag gcggtctcgg caagttcggc cagcacttgg cggtagcggc
     1141 tgagcaagcc gtcgtgttgt tccggggtga gggtttcttg cgccagccgg cgctcgaaca
     1201 cctccagcgg gctgagggcg tccagggtgt cgccggggtc ggcgctcagg ctggccacgg
     1261 cgtcgccgcg ttcgcggcgc acgcgcaaaa tctccaccgg caggccgtca gccagcgccg
     1321 ccacccgcgc ggccaggtcg ctgaggtaat catcgccgcg cacgcgcact tccagccaca
     1381 ccggccgctc ggcgctgcct tgcgccgcca cggcggccaa ttgcgccggc aggctggcca
     1441 ggtcgccgct gagcgaggcc agcgcttgcg cacagggcac cggcagcgcg ctgacttcac
     1501 tgagctgacc ctcggcaaag tccagcagcc acatttgttt gtgggtgtcg gcttcgtcaa
     1561 agctcagcgc cagcggcgag ccgctgtagc ggatgtgatt gagcccgccc acgcactgcg
     1621 ggcggtgaat atgccccagc gccagataat ccactggcgg aaacgcctgg gtgggaaaag
     1681 cctccagcgc gcccacataa atctcgcgca ccgattcgct ggcgctggcc cccaccgtgg
     1741 tcaggtggcc ggtggcgata atcggcagcg ccaggcccag cgcggcgcgg cgggccagcg
     1801 cgcgttgata cagggcgtga tagtgctggg tgatggcggc ttgcagcgcg ccttgcttgt
     1861 cgtcggcgtc ttgcccggcc tggctgcgca gcacatcgcg cgggcggata aacggcgcgg
     1921 cgcacaccag cgcgccgggc tggccgtcgc ggcgcggcag cagcaccact tgctcatcca
     1981 gcggcaaatc agcggacgga atcaccgtgg cgcgcagcgc gcccagcagc gggcggattt
     2041 cgcccagggt ggcggcagaa tcatggttgc cgcccaccac cagcaagctg gccccggcgg
     2101 cggccacccc ggccagcagc tggtgataca gctcgcgcgc atagctgggc ggcgcgccgg
     2161 tgtcgaacac atcgccggcc agcaacaccg cgtcgatgtt cagcgcggaa acctgttcca
     2221 acagccaggc aatcagcgcc cggtgctcgg cctggcgggt tttgccgata aagtgctggc
     2281 ccagatgcca gtcggaggtg tgcaggatgc gcatgggcga agaatgtccg ggtgttggca
     2341 tgacaggatt ctagacggct gccgccggca ggcaaagccc gagatgggct gtttcagcca
     2401 gcgcggctta aagcggctca caaaacccag cgcagcgatc ctggcgtcac gcttttgcca
     2461 atccccacaa ccggctggcg cgcgctacca tgtcgccttg ttcatccacg gcactgactg
     2521 acccatgcct gttccgctcc cgctattgct ccccgaccgg cccgccgcca gcccggcgct
     2581 ggcggcgccg cagttgattg ccgcgcaaag cgacgccgag gcggtgatca cctggctgcg
     2641 cgaatacgaa gattcaccgc acaccttcgc caattaccgc aaagaagccg agcggctatt
     2701 gctgtggtgc ggcgagcgtg ggcaaacgct ggcggggctg gggcgcgaag atattctgga
     2761 ttaccagcgc tttttggcca gcccacagcc ggccgaacgc tggattggcc cggcgcgcgc
     2821 gcgccagcac ccagactgga agccgtttac cggcccgctg gcgccgtcca gcatccgcca
     2881 ggcgctgacc attctgggcg cgctgtttca gtatctgctg gacgccggct atctgcgcgg
     2941 caacccgctg gccctggcgc gccggcaaaa acgcagcctg gcgcagccgc ccggcgtcga
     3001 gcgctatctg gacgccgagt tatggcaggt ggtgcaagac acgctggagg cgctgccgcg
     3061 cagcggcgcg cgcgacctgc accattacga gcgggcgcgc tggctgtttc atctgcttta
     3121 tctgaccggc gcgcggcgca cggaagtggc gcgcgcggtg atgggcgata ttgtccagcg
     3181 ccgggggctg tggtggtggc gggtgctggg caagggcaac aagattggcg atatcccggt
     3241 gggcgacagc ctgctggcgg cgctgcaacg ctaccgcagt cactgcggcc tgccgccgct
     3301 gccgctgccc ggcgaagcca cgccgctggt ggggcgggtg aatcaaccgg gcaagctgga
     3361 cacgctgagc gacaaggcga tttatctgat cgtcaagcag gtgttccgcc tggccgccga
     3421 gcgcgagccg gcccaggccg agcgcctggc cgccgcctct acccactggc tgcgccacac
     3481 tgctgcctcg caccagctgg aagccggcgt gccactgctg gtggtcagcc agaacctgcg
     3541 ccacgccagc atccagacca cgcgcaaata cctgcacacc gaagacgacg cccgccatca
     3601 ggccagccag gcgcatgggc tgggcgaaat ttagcgatag cgtggccaat ggcgcgcacg
     3661 ggtgacggcg cttgcaaagt cagattggcg cagcccgcga ggcccagaag cgccacatat
     3721 gccgcgaaca catgctcagg acggtttttt ctgcaaggcc agcacgatgc gcagttgatc
     3781 ctgctcggtg gccacccgct tgacgccaac gtagtcaaac ggcttgtagc ccatgatctg
     3841 ggtattcaga taggggatca gcgagggcac ttggtccatg cgcaccgaca gcacgtcgcc
     3901 gtcaacggtc agataggggt gtttttcggt gagttgcttg acgccattgc ggccggtggc
     3961 ggtttcgaaa gcgccgatgg caatcgagcc gagcagttgg cccagcaggg tatcgttcag
     4021 cggttcgctg cgttgctgaa agcgcaggcg gatggtgcgc tgcgcccagt ctaccggcag
     4081 gaaggtaaag tccaccgtgt ggcgggcgtc taccgggcct ttggtggtca ccagctcgat
     4141 ggtgcctttt tctggggtca gccgcagatc gcgcacttcc acgccttctt tgttgaagcg
     4201 ttcgcgcagt ttatccagca gataggcgcg cgacagggtg acgccatcgc cgcgcaagcc
     4261 aggcatgcgc gacaggtggt ctttcagcac cagataaaaa tcaataaagc ggtctttttc
     4321 aatggtgacc gtgccgcctt cggcttgcgc cggggcggtc agggtcagcg ccagggcggc
     4381 ggtggccagt acacgcagat aagacatgca aactcccgaa atcatcaatc aaacaccgtg
     4441 cgggcgcctt ccaggcggtc ggcaaaatag cccgaggcca gggtgtccac gcgaatgacg
     4501 ctgttggtgc gcggggcgtg aatgaatttg ccgtcgccga tataaatccc catatgcgaa
     4561 tacggccggt tcatggtgtt gaaaaacacc atatccccgg cgcgcatctt gccggtatcg
     4621 atgggcttgg tggcttcggc gatttgcttg gcattgtgcg gcagttgcac attgacggca
     4681 ttcttgtaga taaacgccgc catgccgctg caatccagcc cggcttccgg gttgctgccg
     4741 ccaaatttat agcccacgtc cagcaggccc agtgcataca tcaccacttc gcgggcgtcg
     4801 cccgaggcct ggatattggc caggctgcgg ttggcattgg gcttggcgct ggcggtgcgc
     4861 gcgggtggtt tgggtttgct ggcggtttgc gttttgcgcg gcgggctgct gctggagcag
     4921 gccgccagcg ccacggccag cagcgcggcc aaggccaggc ttagctggcg cggctttcca
     4981 gttcgtccca gcgcgccagt ttgtccagca gcagggtgtc gatctcgttc acgcggagtt
     5041 gccattcgtg cgcctgtttc gggtcggttt tgtagatgtc cgggttgagc aaggcttctt
     5101 gcagcgcggc ctgttcggct tccagctcgg cgatgtcgtc gggcaggcgc tccagctcac
     5161 gggtttcatt gtaactgagc ttgttgctgc ggcgcgccgg cttggcgctg gcttcggctt
     5221 tgtcggtttt caccggggcg acggccaccg tgcgcgccgg cgccgccagc tcggccatgc
     5281 gcgccttggc agccagccag tcgctatagc cgccggggta ttccatcaac acgccgtcgc
     5341 cttcaaaggc aatcacctgg gtggcgacgt tatccagaaa cgcccggtcg tggctgacca
     5401 aaaacaccgt accgctgtag tcggccagca attgctccag aagttccagc gtgtcgatgt
     5461 ccaggtcgtt ggtgggttcg tccagcacca gcacattggc cgggcgggtg aacagctggg
     5521 ccagcaacag ccggttgcgc tcgccgccgg acagggattt caccgggctt tttgcccgcg
     5581 ccggcgaaaa cagaaaatct tccaggtagc tcatcacatg gcggcgctgg ccgccgattt
     5641 cgacaaaatc cttgccatcg ccaatcacat ccacaatggc catctcgtcg tccagctggc
     5701 tgcggaactg gtcaaagtag gcgatttcca gcttgctgcc gggcttgacc gtgccgctgt
     5761 ccggctggat ttcgcccaga atcagcttga gcaaggtggt tttgccggcg ccattcggcc
     5821 cgatcaggcc aatgcggtcg ccgcgcagca ggcggctggt gaagtcccga atcagcgtgc
     5881 ggccttcgta gcccttgctg acatgctcca gctcggccac cagcttgccc gagcgctcgc
     5941 ccggatccag ccggaaattc acctgaccca cccgctcgcg gcgggcggcg cggtcgcggc
     6001 gcagttgctc caggcgcagc acgcggcctt cgttgcgcgt gcgccgcgcc tccacgcctt
     6061 tgcgaatcca gacttcttcc tgagccagga atttatcgaa cttgcggttt tgctcggctt
     6121 ccaccgccag ctcttctgcc ttgcgcgtct ggtaggcgct gaagctgccg gggtagctgc
     6181 gcagcaggcc gcgatccagc tcaatgatgc gggtggagac attgtccaga aagcgccggt
     6241 cgtgggtgat cagcagcacg ctgccggcaa agtttttcag tagggtttcc agccattcga
     6301 tggcggcgat gtccagatgg ttggtgggtt cgtccagcag cagcacatcc ggcgagcaca
     6361 ccagcgcctg cgccagcgcc acgcgttttt tccagccgcc ggacaattcg ccaatcagcc
     6421 ggtcagccgg caggcccaga tgcgacagcg tgctgtcgat caggttttcc agctgccagc
     6481 cctggcgcgc ttccagctgg ttttgaattt cctgcatggc ggccagcacc gcttcgtggc
     6541 catcgtcgga cggggtggac agcgcctgca cggcgtggtg gtagtcggtc agcaactggc
     6601 gcaaatcgcc caggccctca gccaccgcct caaacaccgt gtgtgccggg tccagcgccg
     6661 gctcttgcgg cacataggcc acccgggtgt cgctctgggc gatgatgcgc ccgtcgtcga
     6721 gcttggccgt gccggcaatc gccttgagca gcgaagactt gccggcgcca ttgcggccaa
     6781 tcagccccac gcgctcgcca ggctcgagag agaagtcaac atgatcgagc aggggcacgt
     6841 gcccgaaggc gaggcaggct ttttcgacgg taatcagcgg catggtggtg aatgtcaggt
     6901 cagcagagcg ggaaattgta gcatgcgcag gcgcagcgcc gacgccatcg atttagagtc
     6961 gctaacaaaa cccagcgcag cgttcctggc taggcgtgtg accgcagaca gtacaagtag
     7021 tacgacaagg tcacacaaca acgccaggag ggttttgtta gcggctctta agcaggccga
     7081 tgcttgggcg cttccagccg ctgggcacga taaatcccct gatggcccga gcaggcatag
     7141 ctggcggcgc aggccagcgc ggcaaacagg ccgatgccgc cgccaaacag ctcgatggcc
     7201 aggagcgtgc aggccagcgg cgtattggcc gcgccggcaa acaccgccgc caggcccagc
     7261 gcggccaata gcggcgccgg cagtggcaat agcgcggcca gcgcattgcc cagcgtggca
     7321 ccgatggcaa acagcggggt gacttcgccg cccttgaagc cacaagccag cgaccacacc
     7381 gtggccgccc acttgcccag cgcctcgccc ggccccaact gacccacaaa cgcggcgtcg
     7441 atcaagttca cccccagccc ctgaaaccgt tcgccgccca gtgcccacac cgtcacggca
     7501 aacagcgcgc cacccagcag cgggcgcagc ggcggatagc tgatgcgcgt tttgaactgg
     7561 cggcccaggc tatgggtggc ccaggcaaag ctgcgcccgg ccaggccaaa caaggcaccg
     7621 gccgccagca cggcaatcag gctgctggcg ctcagcgctg gcagcgcgcc cagtgcgcgg
     7681 tgagcatgct gagcgcccca ggccaggcag acttgatcgg ccagcatcgc cgccagtaag
     7741 cagggcagca acgcatccac ccgcaggcgg ccgatggcca gcacttccag cgcaaacacc
     7801 gcgccggcca gcggcgtgcc aaacaccgag gcaaagccgg cgctgatgcc ggtcatcagc
     7861 acgcgctggc ggccggcggc gtcttgcggc cacagccgat tcagttggtc ggccagcgcg
     7921 ccgcccatct gcaccgccgt gccctcgcgc cccaccgacg cgccaaacag atgcgaaatc
     7981 accgtcgccg ccaaaatcag cggcgccatg cgcaagggaa tggtgttttt cgggtcgtgg
     8041 atttcgtcaa tcagcagatt attgccggcc tccaccggct ggccgacgcg ccaatacacc
     8101 cagccaacgg caaagccggc cagcggcagg ccagccagca gccagggatg ggccagccgg
     8161 gtggcagtgg cccagtccag cgccgccaaa aacagcgccg aagccgagcc ggccagcagc
     8221 gccaccaggg caatcagcag gccggcgctg gcggcgtggc gcagtcgggt gaaccagggc
     8281 agaaaaaagt caacgttcat ggcgtcaggc ggggcccgac aagcgggcaa atcaatcaaa
     8341 aagcgccaaa tctggcggcg caaagtgtag caccccagcg ctgacgcggg gcaggcgcac
     8401 gctgctggcg gccagatttt gcctttgccg gcaaacctcg gtaccctgcc ggccatggtg
     8461 atttttcctg ggatgggcga tgaatgaatt tgacctgatc cgccgttatt ttgaccgccc
     8521 ggcagccgac cccgccgtgc ggctggcggt gggcgacgac gcggcgattt tggcgcccac
     8581 gccgggccat gagttactgg tgtctactga tatgctggtg tgtggccggc atttttttgc
     8641 cgatgtcgat ccgcgcgcgc tggggcacaa ggcgctggcg gtgaatttat ccgacatcgc
     8701 cgccatgggc ggcaccgcgc gctgggccac gctggcagca gcctggcccg aactggacgc
     8761 cgactggctg gcggcgtttg ccgagggctg gtttgccctg gccgacgcgc acgaggtggc
     8821 gctgattggc ggcgacacca cgcgcgggcc gctgaccttg tcggtgagca tttttggcga
     8881 agcccccgct ggccgggcgc tgcgccgcga tggcggccgc gccggcgacg atgtttgggt
     8941 gagcggcgag ctgggcatgg ccgccgccgc cgtgcgcgcc cgcctggggc aactgaccct
     9001 gcccgaggcc gagcgggcct tggcgcagca gcggctggac tggccgcagc cgcgtctggc
     9061 gctgggccag gccttgcgcg gactggccag cgccgcgctg gatgtgtctg atggtttgct
     9121 ggccgacctg ggccacttgg cggcgcgcag tggcgtggcc gcctgggtgc gcgccgactg
     9181 gctgccgatg cctgcgtgcc tgcgcgaaca aggcgcggcc ggcctggcct gcgcggcagc
     9241 gggcggcgat gattatgaac tgtgctttgc cgcaccgccg tcggcgcgcg ccgcgctggc
     9301 cgacctgggc cgcgcgctgg ccgtgccgct gacccgcatc ggcgcgttga ccgaaggcgt
     9361 cggctgccgc ttattagacg ccgacggcgc agagatcctg ctggagcaac atggttatga
     9421 ccatttcgcc tgactggcgc tttatgctgc gccaccccgc ccatgtgatt gccctgggcc
     9481 tgggcagcgg cctggcggcc aaagcgcccg gcacctttgg tacgctggcg gcgctgccac
     9541 tgtacgcgtt gtggctggcg ctggggctga gcccgacgct cttggcctgg ctgctgccgc
     9601 tgctgtttgc gctgggctgc tgggcctgcc agcgcaccgg cgacgcgctg ggcgcgccag
     9661 accacggcgc catggtgtgg gacgaaatgg tggcaatgtg ggcgctattg attgtgctgc
     9721 cggccacgcc gctggcctgg gcgttgggtt ttgcgctgtt tagggtgttc gacattgtca
     9781 aaccctggcc gattcgctgg ctggacgccc agttgaaagg cggctttggc gtgatgctgg
     9841 acgacgcgct ggcggcgctg atggcctggg cggtgtggcg gctggcagcg ccggcgctgg
     9901 gcctgggtta aggcgacggc cggctgcgtc aagcgccgcg cacaaaacgc ttctggcgtt
     9961 gtagcgtttc ttgtctacct gcggtcgcac gcccggccag gagcgcttcg ttgagttttg
    10021 ttagcggctc ttagtcagta gcgctttgcg cagtgcgctt gtgtcagcca ttgtgcatca
    10081 tatttcaaca tcatggtcgg atttctggtt atgctcccgg catattctgt tggcggagtt
    10141 gttcatgtcc gtttcgcttg atacccctga agatattgcc aactggttgc gccggcattt
    10201 tgtgggccag acgtttggtt gtgtgcgctt ttggcaattt gccctggtgc gccccaatga
    10261 ccagtttttt cagctggcca gcgtgacgct ggcgggtgac cggctggact tgctcctgcg
    10321 tcatgccgat ggccagggtg aggccggggt ggtgtcagtg tggcaaccca tgggtctgag
    10381 ccctcgccca catggtgtgg cgctcagcgc tgcggcgcgc ctgagctggg gaaacagcga
    10441 ggcctggcag gccggggaag gccaataccg catccgcacc ccgcggggcg aggggggttt
    10501 tgccattgag ggcgccccag cgctgaccct ggagtgttaa gagccgctca caaaaccccc
    10561 tggcgttgtt gtgtgacctt gccgtactgc
//
LOCUS       DAOPYX010000074        10549 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_7296_length_10549_cov_8.633219, whole genome shotgun sequence.
ACCESSION   DAOPYX010000074 DAOPYX010000000
VERSION     DAOPYX010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 10549)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10549)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10549
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7296_length_10549_cov_8.633219"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(181..2331)
                     /locus_tag="PKN55_05430"
     CDS             complement(181..2331)
                     /locus_tag="PKN55_05430"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNX70713.1"
                     /translation="MWLTRAHSLRFRLIGLGLLINLLIVGMISLVTLDAVHEVARNDL
                     GKQLGTLDRQASAALTRPAAEHDLNTLYWQVEHFSHYPDVVYLAVRDRDGRVLAGGGW
                     APGKLAPEPDRVDRFWLFAPRDQVFHHRLELQVGGQTVGEVVYGLSMADARDALSRAM
                     GWCALVGLFSVVTLFGLQWLLGAWLMRPLRRLGRAARAIADGQYDVTMPEPGHDEIGQ
                     LTDHFQHMRDALRQQIGEIQSQKNMLHAIADYAYAWELWINPAGKLFWTSTASERVIG
                     YTREECMAMPSVLETIAHDEDRARLRHALDHAIEARTSGQGFEFRACHQRGHWLWLTV
                     NWHPIYNTLGGYQGCRLSVMDSSESKHNQLALDKAFADLQALQQLSEVHLRQAERERA
                     RLKALFSALSIGMVFTDRTHRIIYANPMFSRLLSLPAPDALLGEDLSQLTPALFVMPE
                     HSVHGEEEAPRNELKLDDKRVLAFQHIDVHDDEHGSLWIFEDVTAERATAEQLIFLAE
                     RDTLTGVYNRRRFDTELERMVKRADRREQPMALVIFDLNDFKIINDSHGHAAGDQVLI
                     DIARTVNKTVRQNEVFCRLGGDEFAVLMPEASAYDASMLSRRIEQQVTALDFEFDGVK
                     RRVSASLGIAVYPSHANDAQTLVAAADAAMYQAKKQDKRGCWVVFDSSMKLDASQLLH
                     THDYPNQPQRLEPTVFADTGHDDAQPSMIDELFG"
     gene            complement(2549..3859)
                     /gene="argA"
                     /locus_tag="PKN55_05435"
     CDS             complement(2549..3859)
                     /gene="argA"
                     /locus_tag="PKN55_05435"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011136902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004042 - acetyl-CoA:L-glutamate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HNX70714.1"
                     /translation="MSTPEFVQWFREAAPYIHAFRGRIFVIAFGGEVVSQGKFAGLSY
                     DLNLLVSMGVRIVLVHGARPQAEALLAAHGLQTRFHGACRITDPDTLEVVKQAVGVTR
                     ADIEAGLSMALPNSPMAGANLRVAGGNYLVARPMGVRDGVDMQSTGQIRKIDTPGIHA
                     RLDAGELVMLSTLGYSPSGEIFNLTLEEVAAATAIALKAEKLIFLTDTPGIMDQHGEL
                     LRQLTASEAEQFLQEHADEHSDAAVYLPQAIRATRQGVKRAHLISHRTEGALLTELFT
                     HDGIGSMISPEPLVRLREATIDDIGDILSLIEPLERLGTLVKRGREQLEMEIQKFSVL
                     EHDGRIIACVAMNHFADEGMAELACLAVDPEWAGEGYGETLLRHVETQARALNVATLL
                     VLTTQTAHWFVERGFVAGDISELPRAKQKIYNYQRRSKVFFKKL"
     gene            4384..6375
                     /locus_tag="PKN55_05440"
     CDS             4384..6375
                     /locus_tag="PKN55_05440"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNX70715.1"
                     /translation="MLAVMLTVSIGLAVILGFTNIANSHDARRQGEELARQMAARYAE
                     QTEQSLNSSMKVATLLAQTMEGMRQGSMPSRPTLDALQKRILQTNTGFINIWTLFEPN
                     ALDGRDAEFANTPHHDRSGRYVPYFTRKPDGSISQEIPGGGKGEVLEYDRPGDGDYYL
                     VPKQRGVDTVLEPYEYDVAGQKMLLSSFVAPVKDSNGRFIGAAGVDVPLSGVQAALSQ
                     VKPFELGYVSVLSNGGVYIANPDAAKLGKPAQDLPAEALAAVKAGKTFSYQAAGNWMH
                     FLTPIHLGQSGTPWAAMVSVPMDEVLAPAVATRNQSLLIALGCVLALAGVLYLVLSGL
                     LRPLNSLSRAMSYLSSGSGDLTRRMEVTSQDEIGKVAEAFNAFVGQLHSMFSEVRQHA
                     EQLGKHIDELGQVSGQVADGSQRQAEAASATAATIEEVTTSITHIADSARDAEQVVNR
                     TVEFTRESAQVVERSSSEIGQIAGTVRHLNDNLAALESRSTQINAIVNVIRDVADQTN
                     LLALNAAIEAARAGEQGRGFAVVADEVRKLAERTGQATVEISGMITAIQQDTRHAAGE
                     MSVALTQVNQGVELAQQAAQSIASIHGNNSTLIEKIHEIANATAEQSLASTDIARNAE
                     RISSMAADNDGAVQHSAQATRQAQALAASLREIVSHFKL"
     gene            complement(6610..7725)
                     /locus_tag="PKN55_05445"
     CDS             complement(6610..7725)
                     /locus_tag="PKN55_05445"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNX70716.1"
                     /translation="MAPFEELLDKHDVTVFVGEKNKFSTEGVRIPQQALTRRGELRAA
                     LADPAFALAHLRAPYKRFYFYLNSLRAAIDDSFDVMICGDGSRSLNTLATLKPSRRYK
                     LVVSYSENIPVRSIFDAKTDLIKKHAWSAIDLLTPWCEHIRLGMEDEGVTPPIQTIPM
                     GICQDIFQPREKDHALCAQYGLDPDKFIFSYIGKLVSWKGVQYLLYAAKVLKRQGRTN
                     FQIAITGRGAQLDNLKKIIADLGLQEEVVFTGFLPYTEVGRLHNLADCLVLPSVPTLT
                     WQEQFGMVLVEAMACRKPILGSRSGAIPEVSVGASLLHTPGDWRELATDMARLLDDPA
                     LCAQLGETGYRWVCERYTKQHTARQYEAALLRLLAAG"
     gene            complement(8107..8985)
                     /locus_tag="PKN55_05450"
     CDS             complement(8107..8985)
                     /locus_tag="PKN55_05450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014087972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HNX70717.1"
                     /translation="MEIYQLRTFVTVAQQGHLTQAAELLHLSQPAVTAQIKALEEEVG
                     MPLFERTAGGVNLSRIGQELLPQAQAILEASRELLYRSRSLKGQLAGRAVIGAITTPE
                     MINIGPWVAALIEKYPLLDIQLQHGVTGGVLNRVRKKELDAGFYIGKNPYVNVNTVHL
                     TEMRFRVVAPPSWRERIASMSAKELGKLPWVGISQQSSLHKLTSELWREMNISPKKIT
                     EVDHIPCIASLVRAGVGLAIMREDEATAMQHSGDLVLVGDYGKTTDLHFIYSADREGD
                     PIIEALLTELAAMWKH"
     gene            9365..9670
                     /locus_tag="PKN55_05455"
     CDS             9365..9670
                     /locus_tag="PKN55_05455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008954293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HNX70718.1"
                     /translation="MNEDVLKRVQQNPKFAELVHKKTTFGWTLSIVMLVIYYGFILIL
                     AFTPKSLGASLSGGVTTVGMPVGVLIILSAFVLTGIYVRRANTEFDQLNQEIVEESK"
     gene            9671..>10549
                     /gene="actP"
                     /locus_tag="PKN55_05460"
     CDS             9671..>10549
                     /gene="actP"
                     /locus_tag="PKN55_05460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011136828.1"
                     /note="member of the sodium:solute symporter family;
                     cotranscribed with the acs gene which encodes acetyl
                     coenzyme A synthase; mutations affect acetate uptake;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation acetate symporter"
                     /protein_id="HNX70719.1"
                     /translation="MNRWLKYAGYAALALAPVAAFAAGDALQGEVKKAPLNVSAIVMF
                     FAFVAFTLGITYWAARRTRSASDFYTAGGGITGFQNGLAIAGDYMSAASFLGISAMVF
                     EKGYDGLIYSIGFLVGWPVILFLVAERLRNLGKFTFADVASYRLNQTHVRILAATGTL
                     VVVALYLIAQMVGAGKLIQLLFGMNYSSAVVLVGVLMVLYVMFGGMLATTWVQIIKAV
                     ILLSGASFMAFMVLASVGFSPEAMFSKAVEVHDKHAAIMEPGQLVSNPIDAVSLGLAL
                     MFGTAGLPHILMRFFTV"
BASE COUNT         2111 a         3124 c         3151 g         2163 t
ORIGIN      
        1 cgccttcaat tttgcgagaa gacgccccga gtgaacaacc tacagggaaa acacacctag
       61 ccaggaccac tgcgctgggt tttgtgggcg actctaacgg cgagtgtcac ccatgtacgt
      121 aaacggccgg ggcgaactga ccccggccgt gtgttttttg gctgactgac tgtttgatgc
      181 tcagccgaac aattcgtcga tcatggatgg ctgggcgtcg tcgtggccgg tgtcggcaaa
      241 gacagtgggc tccaaccgtt gtggctggtt cggataatcg tgggtatgca gcaattggct
      301 ggcgtcgagt ttcatgctgc tgtcgaacac cacccagcaa ccccgtttgt cctgtttctt
      361 ggcctgatac atggcggcat cggcggctgc caccagggtt tgggcatcgt tggcatgcga
      421 gggatacacc gcaatcccca ggctggcgct cacccggcgc ttgacgccgt cgaattcaaa
      481 atccagcgcg gtcacctgct gttcgatgcg ccgtgacagc atgctggcat cgtatgcact
      541 ggcttctggc atcagtacgg caaattcatc gccacccagc cggcagaata cttcgttctg
      601 tcgcacggtt ttgttcaccg tgcgggcaat gtcgatcaat acctgatcgc ctgcggcgtg
      661 gccgtgactg tcattgatga tcttgaaatc attcaagtca aaaatcacca gcgccatcgg
      721 ttgctcgcgc cggtctgcac gcttgaccat gcgttccagc tcggtgtcga aacggcggcg
      781 gttgtatacc ccggtcagcg tgtcgcgctc ggccaggaag atcagctgct cggcagtggc
      841 gcgctcggcg gtgacgtctt caaagatcca caggctgccg tgctcgtcgt cgtgtacatc
      901 aatatgctgg aaggccagca cacgcttgtc gtccagtttc agctcgttgc gcggagcctc
      961 ttcctcgccg tgcacggagt gctcgggcat gacaaacagt gccggcgtca attgcgagag
     1021 atcttctccc aacagcgcat ccggcgcggg caggctgagc aggcgcgaga acatcgggtt
     1081 ggcatagatg atgcgatggg tacggtcggt gaacaccatg ccgatcgaca gcgcggaaaa
     1141 cagtgctttc agccgggcac gttcgcgctc ggcctggcgc aggtgcactt cgctaagctg
     1201 ttgcaaggcc tgcaagtcgg caaaggcctt gtccagcgcc agctgattgt gtttgctttc
     1261 gctggaatcc atgacactga gccgacagcc ttgatagccg cccagcgtgt tgtagatcgg
     1321 gtgccagttg actgtcagcc acagccagtg gccacgctgg tggcaggcgc gaaactcaaa
     1381 gccctggcca gaggtgcgtg cctcaatggc gtggtccagc gcatggcgca gccgggcacg
     1441 gtcttcgtca tgagcaatgg tttccagcac cgagggcatg gccatgcatt cttcgcgggt
     1501 gtagccgatc acccgctcgg acgccgtgct ggtccagaac agcttgcctg ccggattgat
     1561 ccacaattcc caggcgtagg catagtcagc aatggcgtgc agcatgtttt tttgcgactg
     1621 gatttcgcca atctgctggc gcaaggcatc gcgcatgtgc tggaaatgat ccgtgagctg
     1681 gccgatttcg tcatggccgg gctcgggcat ggtcacatca tactggccat cggcaatcgc
     1741 ccgggccgcg cggcccagcc ggcgcagcgg gcgcatcagc caggcgccca gcagccattg
     1801 caggccaaac agtgtcacta cactgaacag cccgaccagc gcacaccagc ccatggcgcg
     1861 cgacagcgca tcgcgggcgt ctgccatcga caggccatac accacttcgc cgacagtctg
     1921 gccacccacc tgtagttcca gacggtgatg aaacacttgg tcgcgcgggg caaacagcca
     1981 gaagcggtcc acccggtcag gctcgggcgc cagcttgccc ggcgcccagc ctccgccagc
     2041 cagcacgcga ccgtcgcgat cacgtacggc cagatagacc acgtctggat aatgagaaaa
     2101 gtgctcaacc tgccagtaca gggtgttgag atcgtgctca gccgccgggc gcgtcagcgc
     2161 cgccgaggct tggcggtcga gcgtgcccag ctgtttgccc agatcgttgc gtgccacctc
     2221 gtgcacggca tccagggtca ccagagagat catcccgaca atcagcaggt tgatcagcag
     2281 ccccaggccg atcaggcgaa aacgcaatga atgcgcgcgg gtcaaccaca tcacgcaggc
     2341 gtccccgttt gcgcaccagc ggccatgatg gctgcgcacg tgtctgttcg ccggacgcaa
     2401 gccgcccacg ccattcttcg catgacaccg tctccctgtg cgccagaaag cttgccgata
     2461 ttacattggc aagtatcatt cagctatatg attatcgttt tatgttatga aatgcggcta
     2521 tattatctgc atttaccacg aaatccgtct agagcttctt gaaaaaaacc ttggaacggc
     2581 gctggtaatt gtaaattttt tgcttggcac ggggtaattc actgatgtcg cccgccacaa
     2641 acccccgttc aacaaaccag tgtgcggtct gggtggtcag caccaacagc gtagccacat
     2701 tcagtgcccg ggcctgggtt tccacgtggc gcagcagggt ttcaccatag ccttcacccg
     2761 cccattccgg atccacggcc agacaggcca gctcggccat gccttcgtcg gcaaagtggt
     2821 tcatggcaac gcaagcaata atccggccat cgtgttccag cacggagaac ttctggattt
     2881 ccatttccag ctgctcacgc ccgcgcttga ccagggtgcc caggcgctcc agcggctcaa
     2941 tcaaagacaa aatatcgcca atatcgtcaa tggtggcttc gcgcagccgc accagcggct
     3001 cgggcgaaat catcgagccg atgccatcgt gggtgaacag ctcggtcagc agcgcgcctt
     3061 cggtgcggtg gctgatcaaa tgggcgcgtt tgaccccctg acgggtggcg cggatggcct
     3121 gcggcagata caccgccgcg tcgctgtgct cgtcggcgtg ctcttgcaaa aactgctcgg
     3181 cctcgctggc ggtcagctgg cgcagcagct cgccatgttg atccatgatg ccgggcgtgt
     3241 cggtgagaaa aatcaatttt tccgctttca gcgcaatcgc cgtggcggcg gcgacttctt
     3301 ccagcgtcag gttaaaaatc tcgccgctgg gtgaatagcc cagggtggac agcatcacca
     3361 gttcgccggc atccagccgg gcgtgaatgc ctggggtgtc gatcttgcgg atttgcccgg
     3421 tggactgcat atccacgccg tcgcgcacgc ccatcggccg cgccaccaga taattgccac
     3481 cggccacgcg cagattggcg ccggccattg gagaattggg caaggccatc gacaagccgg
     3541 cttcgatgtc ggcgcgagtg acgcccaccg cctgtttgac cacttccagc gtatccgggt
     3601 cggtgatgcg acaggcacca tgaaagcggg tttgcaggcc gtgcgccgcc agcagtgctt
     3661 ctgcctgcgg gcgggcgcca tgcacgagca caatgcgcac gcccatgctg accagtagat
     3721 tcaggtcgta actcaggccg gcaaacttgc cctggctgac cacctcgccg ccaaaggcga
     3781 tgacaaagat gcggccacgg aaagcgtgga tataaggagc ggcttcacgg aaccactgga
     3841 caaactcagg tgtgctcatt ggcggatggc aaacattcgg caagggtgac ggaagtggct
     3901 cacgatagca ggaagcccgc ggccatgcac gtcacagaca gaaaaatgtt gcattgcaag
     3961 attgtgacat ggccagtgcg ccaatccagc catatcagtc aaagcgcatg aaacttccgg
     4021 tcggctggtc cggatgtctt ttcgctgcgg gtttgtagat gaaatacatg tcattctctt
     4081 gtgaacgtgc gtttgaatca ttgacaggaa ttattgaaca aattttcata aaatataggc
     4141 tgaaactcct gaacaaatct ggctatcatg ccgatgtaag ttaagaggga cgcggctttg
     4201 ccgcgaaaac atgccagcgt tccccccggt tggcccctca atgaaataag catatgacat
     4261 taaccgagca ggagctgtca gcgagccccc atgctggcag acatcccgcc tgaaagtacg
     4321 cagcgcaaca ccaccgggag agccctcgaa tgttgaagtc cttgagtttc cgaaaaaaaa
     4381 attatgctcg cggtcatgct caccgtgtcg attggcctgg cggtgatcct tgggttcacc
     4441 aatatcgcca acagccatga cgcccgccgt cagggggaag agctggccag gcaaatggcc
     4501 gcccgttacg ccgagcagac tgagcaatcg ctgaatagct cgatgaaagt ggccaccttg
     4561 ctggcgcaaa ccatggaagg catgcgtcag ggcagcatgc cttcgcgccc gacgctggac
     4621 gcgctgcaaa aacgcatcct gcaaaccaat accggtttta tcaatatctg gacactgttc
     4681 gagcccaatg cgctggacgg gcgcgatgcc gagtttgcca acacgccgca tcatgaccgc
     4741 agcggtcgct atgtgccgta cttcacgcgc aaaccggacg gcagcatcag ccaggaaatc
     4801 cccggcggcg gcaagggtga ggtgctggag tatgaccgcc ccggtgatgg tgattactat
     4861 ctggtgccca agcagcgcgg tgttgacacg gtgctggagc cttatgaata cgatgttgct
     4921 ggccagaaaa tgctgttgtc gtcgtttgtg gcgcccgtca aagacagcaa tggccgcttt
     4981 attggcgcag cgggcgtaga tgtgccgctg agcggcgtgc aggcggcctt gtcgcaggtc
     5041 aagccatttg agctaggcta tgtcagcgtg ctgtccaatg gcggcgtgta catcgccaat
     5101 ccggacgccg ccaagctggg caagccggca caggatttgc cagccgaggc gctggcggcg
     5161 gtgaaagccg gcaaaacctt tagctatcag gctgctggca actggatgca ctttcttacc
     5221 ccgattcatt tgggtcaatc tggcacgccg tgggccgcca tggtgagtgt gccgatggac
     5281 gaagtgctgg cgccggcagt ggccacccgc aatcaatcct tgctgattgc cctaggctgt
     5341 gtgctggcct tggctggcgt gctgtatctg gtgctgtccg gcctgctgcg cccgctgaat
     5401 tcgctgtcgc gcgccatgag ctatctgtcc agcggctcgg gcgacttgac ccggcgcatg
     5461 gaagtcacca gccaggatga aatcggcaag gtggccgagg cgttcaacgc ctttgtgggt
     5521 caactgcata gcatgttcag cgaagtgcgc cagcacgccg agcaattggg caagcatatc
     5581 gacgagttgg ggcaagtatc tggtcaggtg gcagacggtt cgcagcgcca ggccgaagcg
     5641 gccagcgcca ccgcggccac catcgaagaa gtcaccacca gcatcaccca cattgccgac
     5701 agcgcgcgcg acgccgaaca agtggtgaac cgcacggtgg agttcacccg cgaatcggcg
     5761 caagtggtgg agcgttcgtc cagcgaaatc ggccagattg ccggcaccgt gcgccatctg
     5821 aatgacaatc tggcggcgct ggaaagccgc tcgacgcaga tcaacgccat cgtcaacgtg
     5881 attcgtgatg tggccgacca gaccaatctg ctggcgctga acgctgcgat tgaggccgcc
     5941 cgcgccggcg agcaagggcg cggctttgct gtggtggccg acgaggtgcg caagttggcc
     6001 gagcgcaccg gccaggccac ggtggaaatc tccggcatga tcaccgccat tcagcaggac
     6061 acccgccacg ccgccggcga aatgagtgtg gcgctgaccc aggtcaacca aggggtggag
     6121 ttggcccagc aggcagcgca atcgattgcc tcgatccacg gcaataacag cacgctgatc
     6181 gagaaaatcc acgaaattgc caatgccacc gccgagcagt cgctggccag caccgacatc
     6241 gcccgcaacg ccgagcgcat cagcagcatg gcggcggaca acgacggtgc ggtgcaacac
     6301 agcgcacaag ccacccgtca ggcgcaggcg ctggccgcca gcctgcgcga gattgtgtcg
     6361 cacttcaagc tgtagcctgg cgccaccggc ggctattgcc gccgatggct caccctagcc
     6421 ggtaaagcga tcctgatcag gcatgtcagc cgttcagccg cgcatgaacg cctggcgcgt
     6481 tggctgcgcg gcacttagag cggctaacaa aacccagcgc agcgctcctg gctaggcgtg
     6541 cggccgcaga cagtacaaga agtacggcaa ggccgcgcaa cgacgccagg agggttttgt
     6601 tagccgctct taacccgctg ccagcaggcg cagcagcgcg gcttcgtact gacgagcggt
     6661 atgttgcttg gtatagcgct cgcacaccca ccgatagccg gtttcaccca attgtgcaca
     6721 cagggccggg tcgtccagca ggcgcgccat gtcggtggcc agctcgcgcc agtcgcctgg
     6781 ggtatgcagc aggctggcgc cgaccgagac ttccgggatg gcgccagaac gtgagcccag
     6841 aatcggtttg cggcaggcca tggcttctac cagcaccatg ccaaactgtt cctgccaggt
     6901 cagcgtcggc acactgggta ataccaggca atcggccagg ttgtgcagac gtcccacctc
     6961 ggtgtacggc aaaaagccgg taaacaccac ttcttcttgc agccccaggt cggcaatgat
     7021 ttttttcaga ttatccagct gcgcgccgcg tccggtgatg gcgatctgaa aatttgtccg
     7081 gccctgacgt ttgagcacct tggcggcgta gagcagatat tgcacgcctt tccagctcac
     7141 cagcttgcca atatatgaaa agatgaattt atcagggtcc agaccatact gcgcgcacaa
     7201 ggcatggtct ttttcccgtg gctggaaaat atcctggcat atgcccattg gaatggtctg
     7261 aatcggcggg gtcacgcctt cgtcttccat gcccaggcga atatgctcac accacggtgt
     7321 cagcaaatcg atggccgacc aggcgtgctt cttgatcaaa tccgttttgg cgtcaaaaat
     7381 cgaacgcacc ggaatatttt cgctataaga cacgaccagc ttgtagcgcc gtgacggttt
     7441 gagcgtggcc agggtattca gcgagcggct gccatcgcca cagatcatga cgtcaaaact
     7501 gtcatcaatg gcggcgcgta aactattgag ataaaaataa aatctcttgt atggcgcacg
     7561 caggtgggcc aaggcgaagg ccgggtcggc cagcgccgca cgcagttcac cccgtcgggt
     7621 gagtgcctgc tgaggaatgc gcacgccctc ggtggagaat ttgttttttt caccgacaaa
     7681 caccgtgaca tcatgtttgt ccagcaattc ttcaaagggg gccatggtcc atttgctgac
     7741 gccatgctcc cagacaattc caattttcat tgcgggtgtg tttattattg aaccgatttt
     7801 tatttattct cagccaaatg gcgggctgag gctggccggt atcataatat ggttggcgtg
     7861 ttttgcgtgg ccagagagat tgaaatcgca gaaaaaacga ctgaaaaact gctgaagggt
     7921 taaggtgtgt gggcaagtcg ggagatatgc aatatatctg tcatctttgc atcgggttga
     7981 agaggcggca tccaggctgg tagatggcgg tctggccggt tgcgcaacgg caacagccca
     8041 ccggccatga atcatccatg gccggtgggc gatacgctgc gtggcgtgga atccgggcgt
     8101 gccggctcag tgcttccaca tggcggccag ttcggtcagc agcgcctcaa tgatcggatc
     8161 gccttcacgg tcagcactgt aaatgaagtg caggtcagtg gttttgccgt agtcacccac
     8221 cagcaccaga tcaccactgt gctgcatggc cgtggcttcg tcttcgcgca tgatggccag
     8281 gccgacgccc gcgcgcacta ggctggcgat gcaggggatg tggtccacct cggtgatttt
     8341 cttcggcgaa atattcattt cgcgccacaa ctcgctggtg agcttgtgca ggctggattg
     8401 ctgggaaatg cccacccaag gcagttttcc cagttctttg gcgctcatgc tggcaatgcg
     8461 ttcgcgccaa cttggcgggg ccaccacgcg aaagcgcatt tcggtcagat gcacggtgtt
     8521 gacgttcaca taggggtttt tgccgatgta aaacccggcg tcgagctcct ttttgcgcac
     8581 gcggttaagc acgccgccag tgacgccgtg ctgcaactgg atgtccagca gcgggtattt
     8641 ttcgatcagc gcggccaccc aggggccgat attgatcatt tctggcgtgg tgatggcgcc
     8701 aatcaccgcc cgtccggcca gttggccctt caggctgcgc gagcggtaca gcagctcacg
     8761 gctggcctcc aggatggctt gcgcctgcgg cagcagctcc tggccgatgc gcgacaggtt
     8821 taccccgcct gccgtgcgtt cgaacagcgg catgccgact tcttcttcca gcgccttgat
     8881 ttgtgcggtg accgcaggct ggctcaaatg cagcagctct gccgcctgtg tcaggtggcc
     8941 ctgttgcgcc acggtgacaa aggtccgcag ttgataaatt tccatgacga tctctctccc
     9001 cagcaggcag tgatgtgggg ggtatgtctg tgcccgtcct gactgcttgg cagccggcgg
     9061 gcgcatccgg atcgccctgc ctggtcggcc cccggatgct gttatggccc agtcgcccgc
     9121 cgtcatgtgc gggtcgaagg tgtgtttttt agagtgattc tactagaaat ccgaccccct
     9181 tcgccaaccc atcttgccgt cattttgtgt gcataaatta atgtttttaa acaactattg
     9241 ctatgcagca ttcaaatcgc tgattttttt catcagaaat tgcgattagc acgttgacgc
     9301 taacgttaag tagattccac gcaagcaagc ctttccgggg tttgtgtcac aaaggaggaa
     9361 acacatgaac gaagacgttc tgaaacgggt gcaacaaaac ccgaagttcg ccgagctcgt
     9421 ccacaagaag acgacatttg gctggacgct gtcgattgtg atgctggtga tctactacgg
     9481 cttcatcctg attctggcct tcacgccgaa atccctgggc gcttcgctca gcggcggcgt
     9541 gaccaccgtc gggatgccgg ttggcgtgct gatcatcctg tcggccttcg tgctgaccgg
     9601 catctatgtg cgccgcgcca acaccgagtt cgaccaactc aaccaagaaa tcgtcgagga
     9661 gtccaagtaa atgaatcgtt ggctcaagta cgcaggttac gctgcgctgg cgctggcgcc
     9721 agtggcggca tttgcggccg gggatgcgct gcagggcgaa gtgaaaaaag ccccgctgaa
     9781 cgtctcggcc atcgtcatgt ttttcgcctt tgtggccttc accctgggca tcacctactg
     9841 ggcggcgcgc cgcacccgtt cggcctctga cttctacacc gccggcggcg gcatcaccgg
     9901 cttccagaat ggcctggcca tcgcgggcga ctacatgtcc gccgcgtcgt tcctgggtat
     9961 ttccgccatg gtgttcgaaa aaggctacga cggcctgatc tactccatcg gctttctggt
    10021 tggctggccg gtgattctgt tcctggtggc cgagcgtctg cgcaacctgg gcaagttcac
    10081 cttcgctgac gtggcttcct atcgcctgaa tcaaacccat gtgcgcatcc tggcggcaac
    10141 cggcaccctg gtggtggtgg cgctgtacct gatcgcccag atggtgggcg ccggtaagct
    10201 gatccaactg ctgttcggca tgaactacag ctccgccgtg gtgctggtgg gtgtgctgat
    10261 ggtgctgtac gtgatgttcg gcggcatgct ggccaccacc tgggtgcaga tcatcaaagc
    10321 ggtgatcctg ctgtccggcg cttcgttcat ggccttcatg gtgctggcct cggtcggctt
    10381 cagcccggaa gcgatgttca gcaaggctgt ggaagtgcac gacaagcacg ccgccatcat
    10441 ggagccgggc caactggtgt ccaacccgat tgacgctgtg tcgctgggcc tggccctgat
    10501 gttcggcacc gctggcctgc cgcacatcct gatgcgcttc tttaccgtg
//
LOCUS       DAOPYX010000075        10525 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_7318_length_10525_cov_8.951003, whole genome shotgun sequence.
ACCESSION   DAOPYX010000075 DAOPYX010000000
VERSION     DAOPYX010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 10525)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10525)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10525
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7318_length_10525_cov_8.951003"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1113
                     /locus_tag="PKN55_05465"
     CDS             <1..>1113
                     /locus_tag="PKN55_05465"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012695990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HNX70720.1"
                     /translation="IAESVFAVLRRHALLTALTAPQSTPKRLLIASLVRVRGHNLREL
                     SDALTEADRKWAGELKALEPELSLAQRAELPDWVVEALQAGGMDDDTLLTLGRAMQHP
                     APLDLRVNTLKTRREDALAALQAAGISAEATPLSPFGLRVKGKPALTKMPLFTSGQIE
                     VQDEGSQLLGLLLAPRRGEMVVDFCAGAGGKTLLLGAMMASTGRLYAFDISEKRLANL
                     KPRLARSGLSNVHPQLLASENDSRIKRLAGKIDRVLVDAPCSGLGTLRRNPDLKLRQS
                     PDSVAQLNATQASILNAAASLVKPGGRLVYATCSLLPAENDAIVDAFLASHPEFSLLN
                     AEELLAEQRVQLATGERLRLNPARDNTDGFFAVALTR"
     assembly_gap    1114..1213
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1313..1846
                     /locus_tag="PKN55_05470"
     CDS             1313..1846
                     /locus_tag="PKN55_05470"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNX70721.1"
                     /translation="MLRAHRLLLIALALLLGACASPANKNVQITQTARGVEIRSSDKI
                     LFDSGKADIKPEGSAFLDRVASILKTRTRKPVSIEGHTDNVGPADLNRDLSELRALAV
                     MKGLMDRGVPKTRMAYVGYGMSRPIADNSTPEGRMLNRRTEIIILDERKENIGSPLED
                     LAGFFNGVVELGKRLFN"
     gene            1894..2766
                     /locus_tag="PKN55_05475"
     CDS             1894..2766
                     /locus_tag="PKN55_05475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX70722.1"
                     /translation="MTECPLCQTACPPDARFCPKCGHALAVATPPPTPPLLDTEPMPI
                     TPPPQAVTPPAAPAQPQLAASSPSKPPAPARRGHGALLGVLIGLALVLLAMIAAGGWW
                     FTHLSNPATPAGTASAKPAPAPIELAPGLNPTDKTLPAKPATDTTASPATASAAQEAE
                     RALPPPQAKPAPAPSAAPAPASAIRPAPTSAPTPAAPSRPAPAAEPAPQSSSRPVKAD
                     PQLIEPIKPAAKPTPAPVPPEVPASSKSAAPASAPAAQPAPAKPASSQSGSNALHDDI
                     VRRKEALKRQMGVE"
     gene            2882..3199
                     /locus_tag="PKN55_05480"
     CDS             2882..3199
                     /locus_tag="PKN55_05480"
                     /inference="COORDINATES: protein motif:HMM:NF039672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6156 family protein"
                     /protein_id="HNX70723.1"
                     /translation="MTSPLTVRHFVSYSGVKLPLKLHTELDDDGLRHRITFFRGHYTA
                     DESLSRVEKVVYGEIEFTHDYQYHDNGQLSQATVTLHAEEERTVMRFAPDGELLSSDT
                     EAD"
     gene            complement(3348..4430)
                     /gene="nagZ"
                     /locus_tag="PKN55_05485"
     CDS             complement(3348..4430)
                     /gene="nagZ"
                     /locus_tag="PKN55_05485"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003748585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HNX70724.1"
                     /translation="MSVHCPRGPIMVDVAGLSLTDAERQRLLHPLVGAVILFRRNFQS
                     VAQLRALTADIHALRSPALIIAVDHEGGRVQRFIDGFTRLPSMRALGMLWGQSPELAR
                     ARAEAVGEVLAAELRACGVDLSFTPVLDLDWSRCAVIGNRAFHRDPEVVAELALALQT
                     GLLRGGMAACGKHFPGHGWVEGDSHHVIPRDERSLEALRADDLRPFARLAQAGLAAVM
                     PAHVVYPAVDEQAAGFSPRWLQDILRGELGFAGAIFSDDLCMEGASAAGDITARARAA
                     FAAGCDMALVCNRPDLAEQLLAELEMTAPAELASRLAGMAGRGSAADWQAHTASAEFA
                     ALRAQVAALHAQDVAPQTGPAVGEAN"
     gene            complement(4455..4742)
                     /locus_tag="PKN55_05490"
     CDS             complement(4455..4742)
                     /locus_tag="PKN55_05490"
                     /inference="COORDINATES: protein motif:HMM:NF027614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4936 family protein"
                     /protein_id="HNX70725.1"
                     /translation="MSTSLYVYYRVNDDKRAQRLRQWRALAAALAAYQPRRQCRRDDA
                     DTWMEIYPDVSADFAPAYHAVLAALDPDGDWRASRHEEWFIDTDDSPAVPG"
     gene            complement(4739..5611)
                     /gene="ubiA"
                     /locus_tag="PKN55_05495"
     CDS             complement(4739..5611)
                     /gene="ubiA"
                     /locus_tag="PKN55_05495"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008953287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HNX70726.1"
                     /translation="MTLLLKDRLHVYMQLTRLDKPIGSLLLLWPTLWALWLAGDGHPS
                     MLLVSIFTLGTLLMRSAGCVINDYADRHFDGQVERTQHRPFARGVVSEREALLLAAAL
                     ALAAFMLVLPLNLLTWQLSLPALFLAGSYPFTKRFFAIPQAYLGIAFGFGIPMAFAAA
                     QDQVPAIAWLMLLANLCWTVAYDTAYAMVDRPDDLKIGIRTSAITFGRHDVAAIMACH
                     CAFLALMGAVGGQAGLGAGYFAGLAAAGVLVMDQYRMIRERDRQRCFAAFLANNRIGF
                     VVFAGIVLDYWLRG"
     gene            complement(5608..6147)
                     /locus_tag="PKN55_05500"
     CDS             complement(5608..6147)
                     /locus_tag="PKN55_05500"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: protein motif:HMM:NF016248.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008813 - chorismate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HNX70727.1"
                     /translation="MYAFPDWRAAPAENVPAALADWLCETGSLTERLIASGRLFAVEV
                     LAQGVEPAWPDEAAALGLTAGAPALAREVRLTLDGAPFVYARSLCRADSPAWAGVLAR
                     GSRSLGLTLFGEPGIARHPLNFCAGLAAQSALLARARAALPGAGDWLHARRSLFGQPD
                     DALLVHEVFHPLLAEVTPS"
     gene            6270..7655
                     /locus_tag="PKN55_05505"
     CDS             6270..7655
                     /locus_tag="PKN55_05505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014087988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HNX70728.1"
                     /translation="MSAHTPSPAAAETRALLHLAVPIMVAQLAHTATGFVDTVMAGRL
                     GAEALAAVSIGASVMISVFITLSGVAAALNPLVAHHIGAQQPEQVGPTVRQGLWTALA
                     CGLLGALVLLAAGRWGVWMIGLEAPVAASAEGYLQGVAMGMPAALLYRALHAYSSAVS
                     QTRPIMVISLLALALNTVLNYLLIYGKFGFPALGGAGCGWATGVAFWFSLICLAGWTR
                     WRASYRRYALWSAWSWPQWPPQRALLKLGVPIALSYFVEVTAFTSVALLIANLGAQVV
                     ASHQVVINFTSLLYMIPQSLGVALSVRVGHAVGAGDFARARFNSRIGLRLGAAIALCS
                     SLLVFALRAPIAALYSADANVIAMAASLLGFAAVFQLADALQTIASGALRGYKLTTAP
                     MLIHTLSFWGIGLGLGVWLGLLGHGQLAGWTLPLGAAGFWAALTLSLLVAAVLLLALL
                     RHESRRRLGQG"
     gene            7854..8876
                     /gene="murB"
                     /locus_tag="PKN55_05510"
     CDS             7854..8876
                     /gene="murB"
                     /locus_tag="PKN55_05510"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014087989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HNX70729.1"
                     /translation="MPPLFHPDVPLTALNTFGLVARARRLATLSDEAQLPALLADDDF
                     RHGPRLLLGGGSNLLLTHTEVNASVLRIATQGIRLLADDGEQVQVEAAAGESWHGFVR
                     HTLQHGWFGLENLSLIPGTVGASPIQNIGAYGVEVKDRLHSLRALSLDDGQMHEFAAG
                     DCAFAYRDSLFKQQAGRWLVLAVRFTLSRRAAVQIDYGELRQDLAEHGVSAPTPLEVS
                     DAVMRVRQRKLPDPAELGNAGSFFKNPVVDAAHAAALLAQWPDLPHYPQADGRVKLAA
                     GWLIDRAGWKGRRLGPAGVHARQALVLVNHGGATGADIVALAEAIAADVRQRYGVALE
                     AEPVRV"
     gene            9121..9693
                     /locus_tag="PKN55_05515"
     CDS             9121..9693
                     /locus_tag="PKN55_05515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX70730.1"
                     /translation="MPDWIITPASNAMDKIVSYRTVLQAMRQFVRQNAVVSDTVNIYH
                     DAARTQRTSLRYSNNNYQVDQINGQNILYNYPDPLPDFNINTLPSGFPLQGTAVNATQ
                     KSQLLFLLPEAARSEEIQSRMEAAFSNAATIALQPLAVLVKKYSATCAVAGVNVAHPA
                     RPLVRADYLAYANTLPPNNPDRVAILQLLG"
     gene            complement(9908..10234)
                     /locus_tag="PKN55_05520"
     CDS             complement(9908..10234)
                     /locus_tag="PKN55_05520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX70731.1"
                     /translation="MRAVQKITVINKGGYVFNYSVQWLGSDGRWVTSTWNSGNYPVAQ
                     SVTTPPLETIGVPADALAVTVYGHAVLGSSGQGTPFVAYAANDQIATYEAVGTTIIGF
                     AINLIE"
BASE COUNT         1840 a         3527 c         3329 g         1729 t
ORIGIN      
        1 atcgccgaat cggtgtttgc cgtgctgcgc cgccatgcgc tgctgaccgc gctgaccgcg
       61 ccgcaatcca cccccaagcg gctgctgatt gcctcgctgg tgcgggtgcg cggccataat
      121 ctgcgcgaac tttccgacgc gctgaccgaa gccgaccgca aatgggccgg cgagctcaaa
      181 gcgctggagc ccgagctgag cctggcccag cgcgccgagc tgccggattg ggtggtcgag
      241 gcgctgcaag ccggcggcat ggacgacgac accctgctga cgctgggccg cgccatgcag
      301 catccggcgc cgctggattt gcgggtgaac accctgaaaa cccgccgcga agacgcgctg
      361 gccgcgctgc aagccgccgg catcagcgcc gaagccacgc cgctgtcgcc gtttggcctg
      421 cgggtgaagg gcaagccggc gctgaccaag atgccgctgt tcaccagcgg ccagattgaa
      481 gtgcaagacg aaggcagcca gctgctgggc ctgctgctgg ccccgcgccg gggtgaaatg
      541 gtggtggatt tttgcgcggg cgccggcggc aaaaccctgc tgctgggcgc gatgatggcg
      601 tccaccggcc gcctgtatgc gtttgatatt tcagaaaaac gtctggccaa cctcaagccg
      661 cgtctggcgc gctcggggct gtccaatgtg catccgcaac tgttggcctc agaaaacgac
      721 agccgcatca agcggctggc cggcaagatc gaccgcgttc tggtggacgc gccctgctcg
      781 ggcctgggca ccctgcgccg caaccccgac ctgaaactgc gccagtcgcc ggacagcgtg
      841 gcgcagctga acgccaccca ggcctccatc ctcaatgccg ccgccagcct ggtgaagccg
      901 ggcggccgtc tggtgtacgc cacgtgcagc ctgctgccgg ccgaaaacga cgccatcgtc
      961 gacgcctttc tggccagcca cccggaattc agcctgctca acgccgaaga gctgctggcc
     1021 gaacagcgcg tgcagctggc cactggcgag cgcctgcggc tgaacccggc gcgtgacaat
     1081 accgacggat tttttgccgt ggcgctgacc cgcnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnatattga catgggcatg gcaaatattg gtaaattctg tacttttcac
     1261 gcgtcacgcg cgcccgcctg gcgcggcttg cacgtcaatt tccggaggct ccatgttgcg
     1321 cgctcaccgt ctgctgttga ttgccctggc cttgttgctg ggcgcctgtg ccagcccggc
     1381 caacaagaat gtgcagatca cccagactgc tcgcggtgtg gaaatccgct ccagcgacaa
     1441 aatcctgttc gacagcggca aggctgatat caagcccgag ggcagcgcct ttctggatcg
     1501 ggtggccagc atcctgaaaa cccgcacccg caagccggtg tccattgagg gccacaccga
     1561 caacgtcggc ccggctgatc tgaaccgcga tttgtccgag ctgcgcgcac tggcggtgat
     1621 gaaaggcctg atggaccgtg gcgtgcccaa aacccgcatg gcctatgtgg gctacggcat
     1681 gagccggccg attgccgaca acagcacgcc agaaggccgc atgctcaacc gtcgcactga
     1741 aatcatcatc ctggatgagc gcaaagagaa tatcggcagc ccgctggaag atctggccgg
     1801 cttctttaat ggggtggtgg aattgggcaa acgcctgttc aactgacctt tacttcaccc
     1861 cggctgccag gtgcggccgg ccaaggaatc cgcatgaccg aatgcccgct ctgccagacc
     1921 gcctgtccgc ccgacgcccg tttttgcccc aaatgcggcc atgccctggc ggttgccacc
     1981 ccgccgccta ccccgccgct gctagacacc gagccgatgc ccatcacacc gccaccgcag
     2041 gccgtcacac cgcctgctgc gcctgctcaa ccgcaactgg cggcaagcag ccccagcaag
     2101 cctccggcgc ctgcccgtcg tggccatggc gccctgctgg gagtattgat cgggctggcc
     2161 ttggttttgc tggccatgat tgctgcgggt gggtggtggt ttacccatct gtccaacccg
     2221 gccaccccgg caggcacagc cagcgcgaaa ccggcgccag ccccgattga actggcgcct
     2281 ggcctgaacc ccacagacaa gaccctgccg gccaaaccag ccacagacac cacggccagc
     2341 ccggccacgg cctctgccgc tcaagaagcc gaacgtgcac tgcccccgcc gcaagccaaa
     2401 cctgctcctg caccgtcggc ggcaccagcc ccggcaagcg ccattcggcc cgccccgaca
     2461 tcggcgccca ctccagcagc ccccagccgg cccgccccgg ccgccgagcc tgcgccgcag
     2521 tccagcagtc ggcccgtcaa ggctgacccg cagctgatcg agccgatcaa accggcggcc
     2581 aaaccgactc cggctccggt gccaccagaa gtgccggcgt ccagcaagtc tgccgcgccg
     2641 gcttcagccc cggccgcaca accggccccg gccaagccgg ccagcagcca gagtggcagc
     2701 aatgccctgc acgatgacat cgtgcgtcgc aaagaggcgc tgaagcggca aatgggcgtg
     2761 gaataagcct caacaccccg gacggtttgc gccgtccgga agtgccgata accatattgg
     2821 caatatggtt atcggccgat ttgtcaccta tgatggcggc ttcgccccca aggagcctcc
     2881 aatgacttcc ccgctgactg ttcgccattt tgtctcttat tctggcgtca agctgcccct
     2941 gaagctgcat accgagctgg acgacgacgg cttgcgccac cgcatcacat tttttcgtgg
     3001 ccattacact gctgacgaga gcttgagccg ggtggaaaaa gtggtatacg gcgaaatcga
     3061 atttacccac gactatcaat accacgacaa tggccagctc agtcaggcca cggtgaccct
     3121 gcatgccgaa gaagaacgca cggtgatgcg ctttgccccg gatggcgagc tgctgtcgtc
     3181 tgataccgag gctgactgag cgcgggacgc ccctggtacg ccgggtgggc gtggccgcgc
     3241 ggcatggtgt gctgcggcca cccccgctgg ccgggtcgct acgcggggtg tgatcagcaa
     3301 ctcgcccggc cagggtgact gtggcgggtt tgagcggcca gacggggtca gttggcctcg
     3361 cccaccgccg ggccagtctg cggggccaca tcctgggcgt gcagcgccgc cacctgcgcc
     3421 cgcaaggcgg caaattcggc gctggcggta tgcgcctgcc agtcggcggc gctgccgcgc
     3481 ccggccatgc cggccagccg gctggccaat tccgccgggg cggtcatctc cagctcggcc
     3541 agcagttgct cggccaaatc cgggcggttg cacaccagcg ccatatcgca accggcggca
     3601 aacgccgcgc gggcgcgggc ggtgatgtcg ccggctgcgg acgcaccttc catgcacaag
     3661 tcatcgctga aaatcgcccc ggcaaagccc agctcgccgc gcaggatgtc ttgcagccag
     3721 cgcggcgaga acccggccgc ttgctcgtcc actgccggat acaccacatg tgccggcatc
     3781 accgccgcca gcccggcttg cgccagacgg gcaaacggac gcaggtcgtc ggcgcgcagc
     3841 gcttccaggc tgcgttcatc gcgcggaatc acatggtggg aatcgccttc cacccagcca
     3901 tggccaggaa agtgcttgcc gcaggcggcc atgccgccgc gcagcaagcc ggtttgcagc
     3961 gccagcgcca gctcggccac cacctcgggg tcacggtgaa acgcccggtt gccaattacc
     4021 gcgcagcgtg accaatccag atccagcacc ggggtaaagc ttaaatccac gccgcaggcg
     4081 cgcagctcgg cggccagcac ctcgcccacc gcctcggcgc gtgcgcgcgc cagctcgggc
     4141 gattgccccc acagcatacc cagcgcgcgc attgacggca ggcgggtaaa accatcaata
     4201 aagcgctgca cacggccgcc ttcgtggtcc acggcgatga tcagcgccgg cgagcgcagc
     4261 gcgtggatgt cggcggtcaa cgcgcgcagc tgcgccaccg actgaaaatt gcggcgaaac
     4321 agaatcaccg cgccgaccag cgggtgcaac aggcgttggc gttcggcgtc ggtcaggctc
     4381 aggccggcaa cgtccaccat gatcgggcca cgcgggcagt gaacagacat gcaaaactcc
     4441 ttgaaacaag caattcagcc cggcacagcc gggctgtcgt cagtgtcgat gaaccattct
     4501 tcgtggcggc tggcgcgcca gtcgccgtcg gggtcgagcg cggccagcac ggcgtggtag
     4561 gcgggcgcga agtcggcgct gacgtcgggg tagatttcca tccaggtgtc ggcgtcgtcg
     4621 cggcggcatt gtcggcgcgg ctggtaggcg gccagcgccg ccgccagcgc gcgccattgc
     4681 ctcaggcgct gggcgcgctt gtcgtcgttc acccggtaat agacgtagag cgacgtgctc
     4741 atccgcgcag ccagtaatcc agcacgatgc cggcaaacac cacaaaaccg atgcggttat
     4801 tggccaaaaa agcggcaaag cagcgctggc ggtcgcgctc gcggatcatg cggtattgat
     4861 ccatcaccag cacgccggcg gcggccagcc cggcaaaata acccgcgccc agcccggctt
     4921 ggccgcccac cgcgcccatc agcgccagaa aggcgcagtg gcaggccatg atggcggcga
     4981 catcgtggcg gccaaaggtg atggccgagg tgcggatgcc gattttcagg tcgtcggggc
     5041 ggtccaccat ggcgtaggcg gtgtcgtaag ccaccgtcca gcacagattg gccagcagca
     5101 tcagccaggc gatggccggc acctggtcct gcgcggcggc aaaggccatc gggatgccaa
     5161 agccaaaggc aatgcccaga taggcctgcg ggatggcgaa aaagcgtttg gtgaaggggt
     5221 aactgccggc cagaaacagc gccggcaggc tgagttgcca ggtcagcaga ttcagcggca
     5281 gcaccagcat gaacgccgcc agcgccagcg cggcggccag cagcagcgct tcgcgctcgc
     5341 tgaccacgcc acgggcaaac ggccggtgct gggtgcgttc gacctggccg tcaaaatggc
     5401 ggtcggcgta gtcgttgatc acgcagccgg ccgagcgcat cagcagcgtg cccagggtaa
     5461 aaatgctcac cagcagcatg ctggggtggc cgtcgccggc cagccacagc gcccacagcg
     5521 tgggccacag cagcaagagc gagccaatcg gtttatccag gcgggtgagc tgcatataga
     5581 cgtgcaggcg gtctttcagc aacagggtca tgatggggtc acttcggcaa gcaaggggtg
     5641 gaacacttca tgcaccagca gggcgtcgtc gggctggcca aacagcgagc ggcgggcgtg
     5701 cagccagtcg ccggcgccgg gcagcgcggc gcgcgcgcgc gccagcaagg ccgattgcgc
     5761 cgccaggcct gcgcaaaaat tcagcggatg gcgggcaatg cccggctcgc caaacagcgt
     5821 cagccccagc gagcggctgc cgcgcgccag cacgccggcc caggccgggc tgtcggcgcg
     5881 gcacaggctg cgcgcataca caaacggcgc gccatccagc gtcaggcgca cttcgcgcgc
     5941 cagcgccggc gcgccagcgg tcaggcccag cgcggcggcc tcgtccggcc aggccggctc
     6001 gacgccctgc gccagcactt ccacggcaaa cagccggccg ctggcgatca ggcgttcggt
     6061 cagcgagccg gtttcacaca gccagtcggc cagggccgcc ggcacattct cagcaggcgc
     6121 ggcgcgccaa tcgggaaaag catacatggc gcaggattat aaaccagcgt gaggtgctgt
     6181 tgctggcctt gcgcggcaga tgccgtcggg cgcctttgcc ggcgtcgggc gcgccggtat
     6241 actaggctgc ttttttcctg ctgcacccca tgtctgctca tactccctct cctgctgctg
     6301 ccgaaacgcg cgccctgctg cacttggccg tgcccatcat ggtggcccag ctggcccaca
     6361 ccgccaccgg ttttgttgac accgtgatgg ccggccgcct gggcgccgag gcgctggcgg
     6421 cggtgtcgat tggcgcgtcg gtgatgattt cggtgttcat caccctcagc ggcgtggcgg
     6481 cggcgctcaa cccgctggtg gcgcaccata tcggcgccca gcagcccgag caggtcggcc
     6541 ccaccgtgcg gcaaggcctg tggacggcgc tggcctgcgg cctgctgggc gcgctggtgc
     6601 tgctggccgc cgggcgctgg ggggtgtgga tgatcggcct ggaggcgcca gtggcggcct
     6661 cggccgaagg ctatttgcag ggtgtggcca tgggcatgcc ggcggcgctg ctgtatcgcg
     6721 cgctgcatgc ttattcgtcc gccgtcagcc agacccggcc gatcatggtc atcagcctgt
     6781 tggcgctggc gctgaacacc gtgctcaact acctgctgat ctacggcaag tttggttttc
     6841 cggcgctggg cggcgccggc tgcggctggg ccactggcgt ggcgttctgg tttagcctga
     6901 tttgcctggc cggctggacg cgctggcgcg ccagctatcg ccgctatgcg ctgtggtcgg
     6961 cctggagctg gccgcagtgg ccgccgcaac gcgcgctgct gaaactgggc gtgccgattg
     7021 cactgtcgta ttttgtcgaa gtcaccgcct ttacttcggt ggcgctgctg atcgccaatc
     7081 tgggcgcgca ggtggtggcc agccaccagg tggtgatcaa cttcaccagc ctgctgtaca
     7141 tgattccgca aagcctgggc gtggcgctgt cggtgcgcgt gggtcatgcg gttggcgccg
     7201 gcgactttgc ccgcgcccgc ttcaatagcc gcatcggcct gcgcctgggc gcggcgattg
     7261 cgctgtgctc gtcgctcttg gtgtttgccc tgcgcgcgcc gattgccgcg ctgtacagcg
     7321 ccgacgccaa tgtcatcgcc atggccgcca gcctgctggg ctttgccgcg gtgttccaat
     7381 tggccgacgc gctgcaaacc attgcctcgg gcgcgctgcg cggttataaa ctcaccacgg
     7441 cgcccatgct gatccacacc ctgtcgttct ggggcatcgg cctgggcctg ggcgtctggc
     7501 tgggcttgct cggccacggc caactggccg gctggaccct gccgctgggc gcggccggct
     7561 tctgggccgc gctcaccctc agcctgctgg tggccgccgt gctgctgctg gccctgctgc
     7621 ggcatgaaag ccgacgccgg ctgggccaag gctgagccag gctggatgcc cgccagcgcg
     7681 ggaatgacgg ttttttcagc ggtttttaaa gccgcagtcc gtggcggccc tggccgcttt
     7741 ccccttttct ttggcgaaac aaagaaaagg ggtcgggtgc ggggggaacc cccgcccaaa
     7801 atcaaccctc agccttactg cgcgcagcgc agtcaacact gcctagccaa atcatgccac
     7861 ccctcttcca ccccgacgtc cccctcaccg ccctcaacac cttcggcctg gtcgcccgcg
     7921 cccgtcggct ggccaccctc agcgacgaag cgcaattgcc cgccctgctg gccgacgacg
     7981 acttccgcca tggcccgcgc ctgctgctgg gcggcggcag caatctgctg ctcacccaca
     8041 ccgaggtcaa cgccagcgtg ctgcgcatcg ccacccaggg catccgcctg ctggccgacg
     8101 acggcgaaca ggtgcaggtg gaggcagctg cgggcgaatc ctggcatggt tttgttcgcc
     8161 acaccctgca acacggctgg tttggcctgg aaaacctgtc gctgattccc ggcacggtgg
     8221 gcgccagccc gattcagaac atcggcgcct atggcgtgga agtcaaagac cgcctgcaca
     8281 gcctgcgcgc gctgtcgctg gacgacggcc agatgcatga atttgccgcg ggcgactgcg
     8341 cctttgccta ccgcgacagc ctgttcaagc aacaggccgg gcgctggctg gtgctggcgg
     8401 tgcgctttac cctcagccgc cgcgccgccg tgcagattga ttacggcgag ctgcgccagg
     8461 atctggccga acacggcgtc agcgcgccca ccccgctgga ggtttccgac gcggtgatgc
     8521 gcgtgcgcca gcgcaagctg cccgacccgg ccgagctggg caatgccggc agttttttca
     8581 aaaacccggt ggtggacgcc gcccacgccg ccgccctgct ggcgcagtgg ccggatttgc
     8641 cgcactaccc gcaggcggat ggccgggtga agctggcggc aggctggctg atcgaccgcg
     8701 ccggctggaa aggccgcagg ctgggcccgg ctggcgtgca cgcccgccag gcgctggtgt
     8761 tggtcaacca cggcggcgcc actggcgccg atattgtggc gctggccgag gcgattgccg
     8821 ccgatgtgcg ccagcgctat ggggtggcgc tggaggccga gccggtgcgg gtgtgagtgt
     8881 ggcgggtttc acggggtgag cggcgacgcg ttcagaaaga cgcgccaacc cgcctggcgg
     8941 ccatccggcg acagtgcaat aagagtcgct aacaaaaccc agcgcagcgt tcctggctag
     9001 gcgtgtgacc gcagacagta caagtagtac gacaaggtca cacaacaacg ccaggagggt
     9061 tttgttagcg gctctaactg tcgccagccc aagctcagca ccacgccaag gagttttctc
     9121 atgcctgatt ggatcatcac cccagccagc aacgccatgg acaagattgt cagctaccgc
     9181 acggtcttgc aggccatgcg ccagtttgtc cgccagaatg ccgtggtcag cgataccgtc
     9241 aacatctacc acgatgccgc gcgcacccag cgcacttcgc tgcgctattc caacaataat
     9301 taccaggtgg atcagatcaa cgggcagaac attctgtaca actaccctga cccgctgccg
     9361 gactttaata tcaatacctt gccgtctggc tttccgctgc aaggcacggc ggtgaacgcc
     9421 acgcaaaaaa gccagttgct gttcctgctg cccgaggccg cgcgcagcga ggaaatccag
     9481 tcgcgcatgg aagctgcttt cagcaatgcc gccaccattg ccctgcaacc gctggccgtg
     9541 ctggtgaaaa aatactcggc cacctgcgcg gtggcggggg tcaatgtcgc ccatccggcc
     9601 cgtccgctgg tgcgcgccga ttatttggcc tatgccaaca ccctgccgcc caacaacccg
     9661 gatcgcgtgg cgattctgca actgctgggt taagaatcgc tcacagcacc aagcaatcaa
     9721 gttcaagcag aaggtaaaaa aaatcgcgcc cccagatacc tgggggcgcg agggcttgct
     9781 acttactctg acggttgaat ggctaacagc attcaaggcc gcgaccttgg ctgaacgcgc
     9841 gaccgcagac agtgcagcgg aacggccaag ccagacaacc aggccagaag cgttcagtca
     9901 gccacggtta ctctatcagg ttgatggcaa aaccaatgat ggtggtgccc accgcttcgt
     9961 aggtggcgat ctggtcattg gcggcatacg ccacaaacgg cgtgccttgg ccagaagaac
    10021 ccagcacggc gtggccatac acggtaaccg ccagggcatc agccggcacg ccgatggttt
    10081 ccagcggcgg ggtggtgacc gattgcgcca ccggatagtt gccgctgttc caggtggagg
    10141 tgacccagcg gccatcgcta cccagccatt gcaccgagta gttgaacaca tagccgcctt
    10201 tgttgatgac ggtgattttt tgcactgcgc gcataagact ctcctgattt gagaacgtga
    10261 gtgggtgaaa caacagatac aagcaacatc tcatgttgct tttaaaattt ttctactggc
    10321 attttctgtc gccagatgcc ttaaggaagg catctcaaca atcgccatcc ccacaaaaac
    10381 gtggtctcag cgttttgttt tttgcgattg gcaggcatga ttcccctcaa gaaatcagtg
    10441 cttcactcac caggtgcatg gtgtcgagtt ttgtgatcag ctgcagatca tttttgcttg
    10501 tttgccaaat gtggccacac tcacc
//
LOCUS       DAOPYX010000076        10395 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_7447_length_10395_cov_9.571567, whole genome shotgun sequence.
ACCESSION   DAOPYX010000076 DAOPYX010000000
VERSION     DAOPYX010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 10395)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10395)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10395
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7447_length_10395_cov_9.571567"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(76..1359)
                     /locus_tag="PKN55_05525"
     CDS             complement(76..1359)
                     /locus_tag="PKN55_05525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HNX70732.1"
                     /translation="MSTLIIFTLLIVLMLTGMPISISLGLTVLTFFFTMTDIPIESVA
                     LKLFTGIEKFEIMAIPFFILAGNFLTHGGVARRMINFATAMVGHWHGGLGLAGVMACA
                     LFAAVSGSSPATVVAIGSILLPAMVKQGFPNSFGAGVITTSGALGILIPPSIVMVMYS
                     VATNTSVGQLFMAGVVPGLMLAGLLGLTTWYRARKYNYPRLAKASWGQRVKALRESVW
                     GLLLIVVVIGGIYSGIFTPTEAAAMSAVYAFIVAVFVYKDMTLKQVPKVLLDSASMSA
                     MLLYIITNAVLFSFLMTSENIPQSMAAWLLDMGLGPISFLLMVNILLLLAGNVMEPSS
                     IVLIFAPILFPVAQSLGIDPVHFGILITVNMEVGMCHPPVGLNLYVASGITKMGITEL
                     TKAVWPWLLTMIVFLGIVTYIPAVSLWLPRTLGMM"
     gene            complement(1359..1976)
                     /locus_tag="PKN55_05530"
     CDS             complement(1359..1976)
                     /locus_tag="PKN55_05530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HNX70733.1"
                     /translation="MLRFLDHLEEWLIGALMAAATLLIFVAVIHRYGMGIEALQPHLQ
                     HIHLSWAQELCIYMFVWMAKFGAAYGVRTGIHVGVDVLINRMQDNTRAKFIVFGLCAG
                     ALFTAIIGTLGANFVWEIAHTEQTSADLELPMWLVYLAVPCGSYLMCFRFLQVTFSFI
                     RTGELPKHDHSHVEGMDEQTDDINWFAMDDNLHPHGVAHDKREGK"
     gene            complement(2090..3097)
                     /locus_tag="PKN55_05535"
     CDS             complement(2090..3097)
                     /locus_tag="PKN55_05535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HNX70734.1"
                     /translation="MKLKLAAVLVGAALGLSSVVAHAAGEIVIKFSHVVATDTPKGKA
                     AEYFKKLAEERTKGKVKVQVYPNSQLYKDKEELEALQLGAVQMLAPSLAKFGPLGVKE
                     FEVFDLPYIFDDYKELHKVTQGPIGQQLLGKLDSKGIKGLAFWDNGFKQFSANKPLKN
                     PADFKGMKLRIQSSKVLEEEVRSLGAIPQVMAFSEVYQALQTGVVDGTENPVSNFYTQ
                     KMHEVQKHMSLTNHGYLGYAVIVNKKFWDGLPADVRTALDGAMKDATAYANQIAEKEN
                     NGDLEKVKASGKTTIYTPTPAEKAALKQALTPVHAKMAGRIGQDVIDAIYKETGFNQG
                     K"
     gene            complement(3376..4185)
                     /locus_tag="PKN55_05540"
     CDS             complement(3376..4185)
                     /locus_tag="PKN55_05540"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNX70735.1"
                     /translation="MSSTYIHGFSASEQARLLAQADALASVVFGGLDLADAHTLLEVG
                     CGVGAQTRQLLMRWPHMQIHAIDQSPSHLAAAAEHLRAARADGQVRLTRARAECLPLA
                     AAQFDAALTIWVLEHVPEPAQILAEMVRVLTPGGRVILTEVDNSTFRFFPHNPLIEDW
                     WAQFNAVQRQSGADPFIGQQLAALAQAAGLTEICVTPLPIVSSRQTPQRRLALLRYTR
                     DLLLSGADSLKRAGRVSEADEQALLAEFAALEARPEVDFQYLAVRLCARKP"
     gene            complement(4732..6843)
                     /gene="metG"
                     /locus_tag="PKN55_05545"
     CDS             complement(4732..6843)
                     /gene="metG"
                     /locus_tag="PKN55_05545"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008953095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HNX70736.1"
                     /translation="MTAQKRQILVTSALPYANGAIHLGHMVEHIQTDIWVRFQKMRGH
                     DCHYVCADDTHGTPIMLKAEKDGVTPEALIERVHAEHLRDFTGFHIGYDNYYSTNSEE
                     NRHYAYDIYRKLKANGKIVSRTIEQLFDPEKQMFLPDRFVKGECPKCGAKDQYGDSCE
                     VCGATYSPTELKNPYSAVSGATPVLRESEHFFFRLGECADFLREWTAGASTLADGRSQ
                     AHLQTESLNKMNEWIDGGLQDWDISRDAPYFGFEIPDAPGKYFYVWLDAPIGYMASFK
                     NLCERSGLNFDDYFKADSTAELYHFIGKDILYFHALFWPATLQYSGYRTPTGVFAHGF
                     LTVDGQKMSKSRGTFITAQSYLDCGLNPEWMRYYIAAKLSSKIEDIDLSLDDFVARVN
                     SDLIGKFINIASRAAGFISKRFAGQLAAALPQPASLASPVCYAGELEAGAALLAHLQQ
                     QADSIAQALEAREYARALREVMGLADKVNAYVDANKPWELAKQEGQEARLHEVCTVLI
                     NAFRLLMIYLKPVLPKLAADVEAFLNIAPLSWQDASQLLLGHTIQPYQHLMTRIDPKQ
                     IEALIEANKQTVEAAKPTAAAVEPFADTIGIDDFAKLDLRVATVLDCKLVDGSDKLLQ
                     FTVDLGSETRNIFSGIRKAYADPASLIGRKVIVVANLAPRKMRFGVSQGMILCASGVD
                     DGSGLFLLDSDDGAMPGMRVS"
     gene            6922..8049
                     /gene="apbC"
                     /locus_tag="PKN55_05550"
     CDS             6922..8049
                     /gene="apbC"
                     /locus_tag="PKN55_05550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004518942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HNX70737.1"
                     /translation="MNLIKTLFGGGSAAVSPEQVTEHLKSCLDPNTGKDLVSAKAIKN
                     LKIDGGRVSLDVVLGYPARSQWAALAEQVRAHLLTLAGVTEAEVNVSSQIVAHAAQRG
                     VALLPGVKNIIAIASGKGGVGKSTTTVNLALALAAEGARVGILDADIYGPSQPLLLGL
                     QGRRPESPDNKSLLPLESLGVQSMSIGYLVDDDQAMIWRGPMVSQALQQLLNDTRWDN
                     LDYLLIDMPPGTGDVQLTLSQKIPVTGAVIVTTPQDIALLDAKKGLKMFEKVGVPILG
                     LVENMSVHICSNCGHAEPIFGEGGAAKMAEQYGVTVLGQLPLDLSIRLSADQGKPSVA
                     AEPDGEIARRYQAIARQVAIRVAERARDFSSRIPKIVIQNT"
     gene            complement(8237..9109)
                     /locus_tag="PKN55_05555"
     CDS             complement(8237..9109)
                     /locus_tag="PKN55_05555"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HNX70738.1"
                     /translation="MSHPTPDVPDVLPEDTALVLPPCPAVLAGLFEAVQRDHDLREVS
                     SLIAADPAICGSVLRTANSVAEAGHTSLRDPLQAVTKLGVRRVLGIACAIAAPGAFPL
                     PPQVAERFWRLSRTTAEAARQVARRIPGPAEEDAYLYGLFRDCGIPLLMLNYPDYEQT
                     VQDAIAENMPSTKLEYFRYGTHHAMVGGLITRHWKMPAELVLSIRHQHDATLFGDNTG
                     SVAPEIRTLAALGFFASSAARNALGEIEDPEWVWCGRQAAAYLGFQPKEAVELVWDVA
                     EAVREVMGRQHLPE"
     gene            9457..9900
                     /locus_tag="PKN55_05560"
     CDS             9457..9900
                     /locus_tag="PKN55_05560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018747777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease domain-containing protein"
                     /protein_id="HNX70739.1"
                     /translation="MLRIFLCLGLLATVPAWALPACPDVVRAANDKLNPPVQDTRILA
                     DTLSELQRSGRLPADRYVDKRTARAAGWTPGTPFNQIDALKGKRMGGDRFANRERRLP
                     AAEWTEADLDYRGGKRNAKRLVFSARQRFVTTDHYQTFVEIPACQ"
     gene            9891..10169
                     /locus_tag="PKN55_05565"
     CDS             9891..10169
                     /locus_tag="PKN55_05565"
                     /inference="COORDINATES: protein motif:HMM:NF013500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HNX70740.1"
                     /translation="MPITKCDLTRIDSLDTFYSQISSGLKLPAHFGRNLDALEDALND
                     APGPVELFWHDAIAARNDMGEDAFESLTAVLHAVASNRDDFKVWIGLS"
BASE COUNT         2027 a         3150 c         3237 g         1981 t
ORIGIN      
        1 gcggcgctaa acaaaacagg cgtttcgtgc ggggtgtggc tccactcgaa acgcctgttc
       61 tggctcaccg ggctgttaca tcatgcccag ggtgcgcggc agccacagcg acactgccgg
      121 aatataggtg acaatgccca ggaacacgat catggtcagc agccacggcc atacggcctt
      181 ggtcagctcg gtgatgccca tcttggtgat gcccgaggcc acgtacagat tcaggcccac
      241 cggcgggtgg cacatgccca cttccatatt cacggtgatc aggatgccaa agtgcaccgg
      301 gtcgatgccc aggctttgcg ccaccggaaa cagaatcggc gcaaagatca gcacgatgga
      361 cgacggctcc atcacattgc cggccagcag caacagaata ttcaccatca gcaggaagct
      421 gatcgggccc aggcccatgt ccagcagcca ggcggccatg ctttgcggga tgttctcact
      481 ggtcatcagg aaggagaaca gcacggcatt ggtgatgatg tacagcaaca tcgcgctcat
      541 cgacgccgaa tccagcagca ccttgggcac ttgcttgagg gtcatgtcct tgtagacaaa
      601 caccgcgacg ataaaggcgt acacagcaga catggcggcg gcttcggtcg gggtgaagat
      661 gccggagtaa atcccgccaa tcaccaccac gatcaacagc aagccccaca cgctctcacg
      721 caaggccttg acgcgctgcc cccagctggc cttggccaga cgcgggtagt tgtacttgcg
      781 cgcccgatac caggtggtca ggcccagcag gcccgccagc atcaggcccg gcaccacgcc
      841 ggccatgaac agctggccca ccgaggtgtt ggtggccacc gaatacatca ccatcacgat
      901 ggacggcgga atcaggatgc ccagtgcgcc cgaggtggtg atcacccccg cgccaaagct
      961 attggggaag ccttgcttga ccatggccgg cagcaggatg gagccaatcg ccaccacggt
     1021 ggccgggctg gagcccgaca cagcggcaaa cagcgcgcag gccatcacac ccgccagacc
     1081 caggccgccg tgccagtggc ccaccatcgc cgtggcgaag ttgatcatcc gccgcgccac
     1141 gccgccgtgg gtgaggaagt tgccggccag aataaagaac ggaatcgcca tgatctcgaa
     1201 cttctcgatg ccggtgaaca gcttgagcgc caccgactcg atgggaatat cggtcatggt
     1261 gaagaagaac gtcagcaccg tcagaccaag cgagatggaa atcggcatcc cggtcagcat
     1321 caggacgatg agcagagtaa agatgatcag cgtgctcatt atttgccctc ccgtttgtcg
     1381 tgcgccacgc catgcgggtg caggttgtcg tccatggcga accagttgat gtcgtcagtc
     1441 tgttcgtcca tgccttccac gtggctgtgg tcgtgcttgg gcagctcgcc ggtgcggata
     1501 aagctgaagg tcacttgcag gaagcggaag cacatcaggt acgaaccgca gggcacggcc
     1561 aggtacacca gccacatcgg cagctcaagg tcggccgagg tttgttcggt gtgggcgatt
     1621 tcccacacga agttggcgcc cagggtgccg atgatcgcgg tgaacagcgc gccggcgcac
     1681 aggccaaaca cgatgaactt ggcgcgggtg ttgtcctgca tgcggttgat cagcacgtca
     1741 acgccgacgt gaatgccggt gcgcacgccg taagcggcgc cgaatttggc catccacaca
     1801 aacatgtaga tgcacaattc ctgcgcccag gacaggtgaa tgtgctgcag atgcggctga
     1861 agcgcctcaa tgcccatccc gtagcggtga atcaccgcca caaagatcag caaggtcgcc
     1921 gccgccatca gagcgccaat cagccactct tcaagatggt caagaaagcg gagcatgggg
     1981 aaatcctcgt gtcacacgca aaacagcccc ctgccagcgc gtcagcgcag catgcagagt
     2041 ggccacggag cgcccgcctg acgctaggcc aggcggacga tggcgggtat tacttgcctt
     2101 ggttgaagcc ggtttctttg tagatggcgt cgatcacgtc ctggccaatg cggccggcca
     2161 tcttggcgtg caccggggtc agtgcttgct tgagtgcggc tttttcagcc ggggtcgggg
     2221 tgtagatggt ggtcttgcca gaagccttga ccttttccag gtcgccgttg ttttccttct
     2281 cggcgatttg gttggcgtag gcggtggcgt ccttcatcgc gccgtccagc gcggtgcgca
     2341 catcagccgg cagaccgtcc cagaacttct tgttgacgat cacggcatag cccagataac
     2401 cgtggttggt caggctcatg tgcttttgca cttcgtgcat cttctgggtg tagaagttgg
     2461 acaccgggtt ttcggtgccg tccaccacgc cggtttgcag cgcctggtac acttccgaga
     2521 aggccatcac ctgcggaatc gcgcccagcg agcggacttc ttcttccagc accttggacg
     2581 actggatgcg cagcttcatg cccttgaagt cagccgggtt tttcagcggc ttgttggcgg
     2641 agaattgctt gaaaccgttg tcccagaagg ccaggccctt gatgcccttg ctgtccagct
     2701 tgcccagcag ctgctggccg atcgggcctt gggtgacctt gtgcagctct ttgtagtcgt
     2761 caaagatgta cggcaggtcg aacacttcaa attctttcac gcccagcggg ccgaatttgg
     2821 ccagcgacgg ggccagcatc tgcacggcgc ccagttgcag cgcttccagt tcttccttgt
     2881 ccttgtacag ctggctgttc gggtacacct gcaccttcac cttgcccttg gtgcgctctt
     2941 cggccagttt cttgaagtat tctgcggcct tgcccttcgg cgtgtcggtt gccaccacat
     3001 ggctgaactt gatgacgatt tcgccagcgg cgtgcgccac caccgacgac agacccaggg
     3061 cagcgcccac cagcacagcg gcaagtttca gtttcatctc gatttctcct cgacctattg
     3121 tttgacgttc acggacacgg aagccgtgcg tgcccgaaca ataggcaaac tcaagacgcc
     3181 ccgccattgg gggattccgc atcagggtaa accctaatat aaaatcccat ggcggcggtc
     3241 cgctgcttta gccgaactaa gagccgctca cagaaccccc tggcgttgtt gtgtgacctt
     3301 gtcgtacttc ttgtactgcc tgcggtcaca cgcctagcca ggaccgcttc gctgggtttt
     3361 gttagcggct ctaaatcacg gtttgcgcgc gcacaaccgc accgccagat actgaaaatc
     3421 cacctccggg cgtgcttcca gcgcggcaaa ctccgccagc aaagcctgtt catcggcctc
     3481 gctcacccgc ccggcgcgct tgaggctgtc cgcgccagac agcaataaat cacgggtgta
     3541 gcgcagcaag gccagccggc gctgcggcgt ctgccggctg gagacaatcg gcaacggcgt
     3601 gacgcagatt tccgtcagcc ccgccgcctg cgccaatgcc gccaattgct ggccaataaa
     3661 cggatccgcc ccggattggc gctgcaccgc gttgaactgc gcccaccaat cttcaatcag
     3721 cggattgtgc ggaaaaaacc gaaaagtgct gttgtccacc tcagtcagaa tcacccgccc
     3781 gccgggcgtc agcacgcgca ccatctcggc cagaatctga gccggctcgg gcacatgctc
     3841 cagcacccaa atggtcagcg ccgcatcaaa ctgcgccgcc gccagcggca ggcactccgc
     3901 ccgcgcgcgg gtcaagcgca cctgaccatc ggcccgcgcc gcgcgcaaat gctccgccgc
     3961 cgccgccaga tggctggggc tttggtcgat ggcgtgtatt tgcatatgcg gccagcgcat
     4021 caacaactgg cgggtttgcg cccccacccc gcagcccact tccagcaggg tgtgcgcatc
     4081 ggccaaatcc aggccgccaa acaccaccga cgccagcgcg tcggcctgcg ccagcaagcg
     4141 ggcttgctcg ctggcggaaa aaccgtggat atatgtagag gacatcagcg tgctcgatct
     4201 tctatcagga aaaggctgca atattatact gacgtcatcg aggttgggcg tgaagaatcc
     4261 gtcaaagagt gtataggtgg gagattctgg aacacaggag atgctgcgcc tcgcaccggg
     4321 cggactgtca ggttgcagct cgcccagcat gatgatggcg cgcagacgaa agcagctcac
     4381 caaccgtgcc tggcaggctt ggcagcacgc ctgacgcggt ttggtgagcc gcgctgagca
     4441 gatatgccgc cgctggcacg gccggcgagg caaatctgtc agaagggagg gttcagcggt
     4501 gtttagcgtc gctcacaaaa ctcagcgaag cgatcctggc taagagccgc taacaaaacc
     4561 cagcgcagcg atcctggcta ggcgtgtgac cgcagacagt acaagcagta cggcaaggtc
     4621 acacaacaac gccagggggt tttgttagcg gctctaagtg tgcagccgca gacagtacgg
     4681 gtagtacggc aaggtcgcgc aacgacgcca ggagcgtttt gtgagtggcg cttaactcac
     4741 ccgcatcccc ggcatcgcgc catcatcact atccagcaaa aacagcccgc tgccgtcgtc
     4801 cacgccgctg gcgcacagaa tcatgccctg cgacacgcca aagcgcattt tgcgcggggc
     4861 gaggttggcc accacgatca ccttgcggcc gatcaggctg gccgggtcgg cgtaggcttt
     4921 gcggatgccg gagaagatat tgcgcgtttc gctgcccaga tcgacggtga attgcaagag
     4981 cttgtccgag ccgtccacca gcttgcagtc cagcacggtg gccacgcgca aatccagctt
     5041 ggcgaagtcg tcgatgccga tggtgtcggc aaacggttcg acagcggcgg cggtcggttt
     5101 ggcggcttcc acggtttgct tgttggcttc gatcagcgcc tcgatctgtt tcgggtcgat
     5161 gcgggtcatc aggtgctggt agggctggat ggtgtggccc agcagcagct ggctggcgtc
     5221 ttgccagctc agcggggcga tgttgaggaa ggcttccaca tcggcggcca gcttgggcag
     5281 caccggcttg aggtagatca tcagcaggcg gaaggcgttg atcagcacgg tgcacacttc
     5341 gtgcaggcgc gcttcctggc cttcctgctt ggccagctcc cacggcttgt tggcgtccac
     5401 ataggcgttg accttgtcgg ccaggcccat cacctcgcgc agcgcgcggg cgtattcgcg
     5461 cgcttccagc gcctgggcga tgctgtcggc ttgctgttgc aggtgggcca gcagcgcggc
     5521 gccggcttcc agttctccgg cgtagcacac cgggctggcc agcgatgcgg gctgcggcag
     5581 cgcggcggcc agttggccgg caaagcgctt gctgataaag ccggcggcgc ggctggcgat
     5641 attgatgaat ttgccaatca ggtcgctgtt gacgcgggcg acaaagtcat ccagcgacag
     5701 atcaatatct tcaatcttgc tgctgagctt ggcggcgatg taatagcgca tccattccgg
     5761 gttcagcccg cagtccagat agctttgcgc ggtgatgaag gtgccgcgcg acttggacat
     5821 tttctggccg tccacggtca aaaagccgtg ggcaaacacc ccggtcggcg tgcggtagcc
     5881 agaatattgc agggtggccg gccagaacag cgcgtggaaa tacaggatgt ccttgccgat
     5941 gaagtggtac agctcggcgg tggagtcagc cttgaagtag tcgtcaaagt tcaggccgct
     6001 gcgttcgcac agattcttga agctggccat atagccaatc ggcgcgtcca gccacacata
     6061 gaaatacttg cccggcgcat cggggatttc aaagccgaag tacggtgcat cgcgcgagat
     6121 gtcccaatct tgcaggccgc cgtcgatcca ctcgttcatc ttgttcagcg actcggtttg
     6181 caagtgcgcc tggctgcggc cgtcggccag cgtgctggcg ccagcggtcc actcgcgcag
     6241 gaagtcggcg cattcgccca ggcggaagaa gaaatgctcg ctctcgcgca gcaccggcgt
     6301 ggcgccggac accgccgagt aggggttttt cagctcggtc gggctgtagg tggcgccgca
     6361 cacctcgcag gagtcgccgt attggtcctt ggcgccgcac ttggggcact cgcccttgac
     6421 gaagcggtcg ggcaggaaca tctgtttttc cgggtcgaac agctgctcga tggtgcggct
     6481 gacgatcttg ccgttggctt tcagcttgcg gtagatgtcg taggcgtaat ggcggttttc
     6541 ttcgctattg gtgctgtaat agttgtcgta gccgatgtga aagccggtga agtcgcgcag
     6601 gtgctcggcg tgcacgcgtt cgatcagcgc ctcgggggtg acgccgtctt tctcggcctt
     6661 gagcatgatc ggcgtgccat gggtgtcgtc ggcgcagaca taatggcagt cgtggccgcg
     6721 cattttttga aagcgcaccc agatgtcagt ctggatatgc tcgaccatat ggccaaggtg
     6781 gatggcgccg tttgcgtagg gcagggcgct ggtcacaaga atctggcgtt tctgggcagt
     6841 catgggattc cgaatcttgt aaaaagcagt aaaagcccta ttgtaacaag ttgacccgcc
     6901 acacccaatg gaacctccag catgaatctg atcaaaaccc tgtttggcgg cggcagcgcc
     6961 gccgtcagcc ccgaacaggt gactgaacat ctgaaatcct gcctcgaccc caacaccggc
     7021 aaggatctgg tcagcgccaa ggccatcaaa aacctcaaga tcgacggcgg ccgcgtcagc
     7081 ctggacgtgg tgctgggcta cccggctcgc tcgcagtggg cggcgctggc cgagcaggtg
     7141 cgcgcgcatc tgctcaccct ggccggggtg actgaggccg aagtcaacgt cagcagccag
     7201 attgtcgccc atgccgccca gcgtggcgtg gcgctgttgc ctggcgtgaa aaacatcatc
     7261 gccattgcct cgggcaaagg cggggtgggc aagtccacca ccacggtgaa cctggcgctg
     7321 gcgctggccg ccgaaggcgc gcgggtgggc attctcgacg ccgacatcta cggcccgtcg
     7381 caacccctgc tgctgggcct gcaaggccgc cggccggaat cgccggacaa taaatcgctg
     7441 ctgccgctgg aaagcctggg cgtgcagagc atgtcgatcg gctatctggt ggacgacgac
     7501 caggcgatga tctggcgcgg gccgatggtc agccaggcgc tgcaacagct gctgaacgac
     7561 acccgctggg acaatctgga ctatctgctg atcgacatgc cgccgggcac cggcgatgtg
     7621 caactgacgc tgtcgcaaaa aatcccggtg accggcgcgg tgattgtcac caccccgcaa
     7681 gacatcgccc tcctggacgc caagaagggc ctgaagatgt tcgagaaggt cggcgtgccg
     7741 attctgggtc tggtggaaaa catgagcgtg cacatctgct cgaactgcgg ccacgccgag
     7801 ccgatttttg gcgaaggcgg cgcggcaaaa atggccgagc aatacggggt gaccgtgctg
     7861 ggccaactgc cgctggattt gtccatccgc ttgagcgccg accagggcaa gcccagcgtg
     7921 gcggccgagc cggatggcga aatcgcccgc cgttatcagg cgattgcccg gcaggtggcg
     7981 attcgcgtgg cggaacgggc gcgggatttt tcttcgcgca ttcccaagat cgtgattcaa
     8041 aatacctgaa atatcttgcg ccatttatcc aggtaaataa aaaaccgccg ggtctggcgg
     8101 ttttttattt tggcgagatg gcctgcgcag caagtttagc gtcgggcata aaactcagcg
     8161 aagcggtcct ggctcggcat gtgcaaacgt gcggcaaggt tacaccataa cgccaggagg
     8221 ggtttatcag cggcgcttat tcgggcaaat gctggcgccc catcacctcg cgcaccgcct
     8281 cggccacatc ccacaccagc tcgacggctt ctttgggctg aaagcccaga taggccgccg
     8341 cctgacggcc gcaccacacc cattccgggt cttcgatttc gcccagggcg ttgcgcgcgg
     8401 cggaagaggc aaaaaagccc agtgccgcca gcgtgcggat ttcaggggcc accgagccgg
     8461 tgttgtcgcc aaacaaggtg gcgtcgtgct ggtggcggat cgacagcacc agttcggccg
     8521 gcattttcca gtggcgggtg atcaagccgc ccaccatggc gtggtgggtg ccatagcgga
     8581 agtactccag cttggtggat ggcatgtttt cggcgatggc gtcctgcacg gtttgctcat
     8641 aatccgggta gttcagcatc agcagcggga tgccgcaatc gcggaacagg ccgtacagat
     8701 aggcgtcttc ttccgccggg ccggggatgc ggcgcgccac ttggcgggcg gcttcggccg
     8761 tggtgcgcga caggcgccag aagcgctcgg ccacttgtgg cggcagcgga aatgcgcccg
     8821 gcgcggcaat cgcgcaggca atgcccagca cccggcgcac gcccagcttg gtcaccgcct
     8881 gcaaggggtc gcgcagggag gtatgcccgg cttccgccac gctgttggcg gtgcgcagca
     8941 ctgaaccaca aatggcggga tcggcggcga tcagcgagga aacttcacgc agatcgtgat
     9001 ctcgttgcac ggcttcaaac aatccggcca gcactgccgg gcacggtggc aagaccaggg
     9061 cagtgtcttc gggaagcaca tcgggaacat ccggagtggg gtgcgacatg tatttctccc
     9121 tgcgttttca gttcggggcc acgcagatgg ctgattctgt agaggccggc aaaaccggca
     9181 aagcgccatg gacgaatcca gcctgacacg ggggtggcgg agccaagcgg cactccggcg
     9241 ccagctgctt ggctgacaca ggaaagcgcg caaaaatgca tctgaacgcc ggattttatc
     9301 gaggcgtcca agcgtgccac agctggggat ttctctcaag ccatccatcg gacaatttga
     9361 ccaagcattt gatgtgaatc atgaaacatt tgtcatggat tgctgcattg gcgtgaacct
     9421 tttacgataa gacttttact ggagtgaatt tatcccatgc ttcgaatttt cctgtgcctt
     9481 ggcttgctgg ccaccgtgcc cgcctgggcc ctgccggcat gtccggatgt ggtgcgcgct
     9541 gccaatgaca aactgaatcc tccggtgcag gacacccgca tcctggcgga caccctgtcc
     9601 gagttgcaac gcagcggccg cctgccggca gatcgttacg tagacaagcg cacggcccgt
     9661 gctgccggtt ggacaccggg cacgccgttc aatcagattg atgcgcttaa gggtaagcgc
     9721 atgggcggcg accggtttgc caatcgcgag cggcgcttgc cggccgccga atggaccgag
     9781 gccgaccttg attaccgcgg gggcaagcgc aacgccaagc ggctggtgtt ttctgctcgc
     9841 cagcgctttg tgaccactga ccattaccag acctttgtcg agattccagc atgccaataa
     9901 caaaatgtga cctgacccgt attgactctc tggacacttt ttatagccaa atcagcagtg
     9961 gattgaaact gcctgcgcac tttggccgca atctggatgc gctggaagac gcgctcaatg
    10021 acgcgccagg cccggtggag ctgttctggc acgacgccat cgccgcgcgc aatgacatgg
    10081 gtgaagatgc ttttgaatcg ctgaccgccg tgctgcatgc ggtggccagc aatcgggatg
    10141 acttcaaggt gtggatcggc ctgtcttaag agccgctcac aaaaccccct ggcgttgttg
    10201 tgtgaccttg ccgcactttg ttgtactgtc tgcggtcaca cgcctagctg gagcaactca
    10261 cgctgagcgt ctgcgcccaa tccggcggca gcgcagccag ctccgcgttg gcggggtgtt
    10321 cgcggtaggg gtcggccagg gcggcgcgca gccgctcaat cggttgataa tcgccctgct
    10381 gcgcggcctg aatgg
//
LOCUS       DAOPYX010000077        10331 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_7511_length_10331_cov_13.110841, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000077 DAOPYX010000000
VERSION     DAOPYX010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 10331)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10331)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10331
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7511_length_10331_cov_13.110841"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..336
                     /locus_tag="PKN55_05570"
     CDS             <1..336
                     /locus_tag="PKN55_05570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012698548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HNX70741.1"
                     /translation="GREERRAAAETAEGIIQEKTREFSQWLAGRDTVPVIRALQVEAE
                     RVRRHALEAAAKRLHKGEAPEDVLEFLSRQLTNKLLHPPLQALNSHHGQAKAELTDAL
                     ARLYNLRPD"
     gene            479..1555
                     /gene="prfA"
                     /locus_tag="PKN55_05575"
     CDS             479..1555
                     /gene="prfA"
                     /locus_tag="PKN55_05575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003790927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HNX70742.1"
                     /translation="MKASIASKLDQLVDRLDEVTRLLSTEEATRDMESFRKLTREHAE
                     LTPVVEQYQAFRQCEADMRAAEELLADPDMRELAQAELEDGKVRLGELELALQKLLLP
                     RDPNDERNIFLEIRAGTGGDESALFAADLFRMYSRFAERNRWQVEIVSASESDLGGYK
                     EIIARLVGFGAYSKLKFESGGHRVQRVPATETQGRIHTSACTVAVMPEADEVEAVNIN
                     PAELRIDTFRASGAGGQHINKTDSAVRVTHLPTGIVVECQDDRSQHKNKARALAVLAS
                     RIMDAQLRERQAKEAATRKSLVGSGDRSERIRTYNFPQGRVTDHRINLTLYKLDQVMD
                     GDLFELTEALSAEHQAEQLAAMGE"
     gene            complement(1647..2687)
                     /gene="pyrC"
                     /locus_tag="PKN55_05580"
     CDS             complement(1647..2687)
                     /gene="pyrC"
                     /locus_tag="PKN55_05580"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018746166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HNX70743.1"
                     /translation="MTDTLTLTRPDDWHLHLRDGAALTAVLPDTARQFGRAIVMPNLK
                     PPVTTVADAEAYRARILAALPAGMTFEPLMTLYLTDRTTPDEIVRAKASGIVHGVKLY
                     PLGATTNSDSGVSSLEHCLPVLEKMAEVGLPFLLHGEVTDPDIDIFDREAVFIERTLA
                     PLIARLPELRVVLEHITTAQAAEFVASASANVAATLTAHHLLMNRNAMLVGGIRPHHY
                     CLPVLKRETHRQALLKAATSGSAKFFLGTDSAPHARHTKEAACGCAGMYTAHAALELY
                     AEAFDSVGALDKLEAFASFNGPDFYRLPRNSGTVTLRRESWTVPATLAYLDDAPLVPL
                     RAGETLAWKLQG"
     gene            complement(2772..3002)
                     /locus_tag="PKN55_05585"
     CDS             complement(2772..3002)
                     /locus_tag="PKN55_05585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008954716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HNX70744.1"
                     /translation="MTADKDIDLSGLNCPLPILRAKKALADMSSGQVLRVTATDPGAP
                     GDFAAFCRQTGNTLEDSIVTDEGKFVMVLRRK"
     gene            3058..3828
                     /locus_tag="PKN55_05590"
     CDS             3058..3828
                     /locus_tag="PKN55_05590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012698569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NRDE family protein"
                     /protein_id="HNX70745.1"
                     /translation="MCLIGIAYQHLPGTPLLLLANRDEYYARPARPIDRWEDAPHILG
                     GRDGQAGGSWLAVSESGRVAAVTNVRTGSRTPGRRSRGELVQAWLQDSRPAAEFAGWL
                     QAQRGEYAPFNLLFGCLNDLYCFHSPRALLSRLRPGVHVLSNGLPDAPWPKSERLRGW
                     MSAMHSLPADEVMLDWLADRTPAPVEALPNTGVGLALEKFLSPVFIAGDDYGTRASTV
                     LTLGSKGRGVLVERVFHAGRPQGSRRLSFAVQAGARAC"
     gene            3861..4370
                     /locus_tag="PKN55_05595"
     CDS             3861..4370
                     /locus_tag="PKN55_05595"
                     /inference="COORDINATES: protein motif:HMM:NF020331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNP1-like family protein"
                     /protein_id="HNX70746.1"
                     /translation="MRRFSLLAALAVAALSPLAQAEYRNDMFNQNYVWETEKPWEEKT
                     VELPAYPDGKHWQEFEVGPTTRNRHAVDTATLRLDDDGVVRFAVRVQSPDGAETLTYD
                     GLRCETREHKRYAIGAENKTWAPSRSSQWRRLESGQAVLRALEEFYLCVDRSPVASVN
                     EAIRRMKQR"
     gene            complement(4385..5425)
                     /locus_tag="PKN55_05600"
     CDS             complement(4385..5425)
                     /locus_tag="PKN55_05600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003775685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HNX70747.1"
                     /translation="MEIPDLLAFARSQHASDLHLSAGLPPLLRVHGDIRPIALPALSA
                     AEVRALLHSMMDEGQRRQYAEAHECDFALAWPDSGRCRVNVFSQQRGPAAAIRLIPEH
                     APALEHLGAPPAVCALDRLPHGLVLVTGATGSGKSTTLAALLARINARRAAHILTIED
                     PIEFIHPCQLSVVHQREVGRHTHGFATALRAALREDPDVILLGEMRDADTMRLALTAA
                     ETGHLVLATLHTQSAAQAPDRVINSLPAAERDMARAVLADCLRAVVAQTLIKTADGAR
                     RVAAFELLLANPAVRHHLREGDAAQLYSAMQTGQAQGMQTLDQALLALCGQGVIDAEA
                     ARALARDAALFA"
     gene            5533..6228
                     /locus_tag="PKN55_05605"
     CDS             5533..6228
                     /locus_tag="PKN55_05605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014088766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HNX70748.1"
                     /translation="MTIESALRATRERLAQAEAAAGREAGSVRLLAVSKTFPAEAVRE
                     AAAAGQQCFGENYVQELAAKAVELAELGLEWHFIGPLQSNKTRPVAECAHWVHSIDRL
                     KIAERLSAQRPQNMPDLQVCLQVNVSGEASKSGVSPAEIPALAAAVAALPRLRLRGLM
                     CIPEATDDLPRLREQLAVLPQLRAQLAEQGLALDTLSMGMSADLELAVACGSTLVRVG
                     TAIFGGRTPRHPA"
     gene            6254..>6891
                     /gene="proC"
                     /locus_tag="PKN55_05610"
     CDS             6254..>6891
                     /gene="proC"
                     /locus_tag="PKN55_05610"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012695525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HNX70749.1"
                     /translation="MMHITFIGGGNMATAIIAGLTKARGCQISVVEPNADKRAQLAQQ
                     FGVTTQEQLPVYFGSGQIVVLAVKPQQLREVCGGLASRLDGALVLSIAAGVGVSALSR
                     WLDGHERIVRIMPNTPAMIGKGVAGLYAPRGVDEQDRDAAQRIMAAAGSTVWLDEESG
                     IDDITAISGSGPAYVFYFIEGLIDAARQMGFDDATARELALGTFEGAVALAR"
     assembly_gap    6892..6991
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6992..7468
                     /locus_tag="PKN55_05615"
     CDS             <6992..7468
                     /locus_tag="PKN55_05615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HNX70750.1"
                     /translation="HPLAQFTVTLTNFAVIPIRRLVPSVRGYDSATLLLAWLVTLVST
                     LVLLWLSPFPYNFAAPQSWLVLALLATLKVFVLSLYLLMGALVVQAILSWVNPYNPLA
                     PILDSLTRPYLRPFERLRIGAVDLGPLVLLLILQMLVGIPLARLEWLLLSQMQMLM"
     gene            7569..7874
                     /locus_tag="PKN55_05620"
     CDS             7569..7874
                     /locus_tag="PKN55_05620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014088764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HNX70751.1"
                     /translation="MKKLLVSAAALALLASAPAFANQALAQKNNCLSCHAVDKKVVGP
                     AYKEIAAKYKGDKTAEAKLVEKVKKGGVGAWGQIPMPANAQVSDADVKALVKWILSM"
     gene            8105..8515
                     /gene="dksA"
                     /locus_tag="PKN55_05625"
     CDS             8105..8515
                     /gene="dksA"
                     /locus_tag="PKN55_05625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019899 - enzyme binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HNX70752.1"
                     /translation="MAKLTEQDILNYDGDDYMNADHLEFFKDRLLQMQQELLSNANAT
                     TSHLQEQEATPDPADRATLEEEYALELRTRDRERKLLLKIQSTLKKIDAGDYGYCEDT
                     GEPIGLKRLLARPTASLSVEAQERRERMKKQYAD"
     gene            8720..9208
                     /gene="purE"
                     /locus_tag="PKN55_05630"
     CDS             8720..9208
                     /gene="purE"
                     /locus_tag="PKN55_05630"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HNX70753.1"
                     /translation="MIQVGVVMGSNSDWEVMQNAARVLKDFGVAFETRVVSAHRTPDL
                     LFEYAETAEARGLQAIIAGAGGAAHLPGMLAAKTHIPVLGVPVPSKYLRGEDSLLSIV
                     QMPKGIPVATFAIGEAGAANAALFAVAMLANQSPELAAALKAFRQKQADTVLAMTLPE
                     VL"
     gene            9208..>10331
                     /locus_tag="PKN55_05635"
     CDS             9208..>10331
                     /locus_tag="PKN55_05635"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HNX70754.1"
                     /translation="MQAILPPAMLGILGGGQLGRMFVTAAKRMGYRVTVLDPDAQAPA
                     AEFADVHLCAAYNDAEALRTLAASCAAITTEFENVNADAMRELAKTTRVSPSGDCVAI
                     AQDRIAEKAWVNKAGLPTAPYLAIESVEDIQVELAPYLPGILKTARLGYDGKGQVRVN
                     SADEARAAYANLGGQACVLEKMLDLKLEVSAIVTRVSPEQLSVFPVAENIHKNGILDE
                     SIVPARIAPALAAQAQQMARQLAEALDYVGVLAVEFFVLADDSLVINEIAPRPHNSGH
                     YTIDACVSDQYQQQVRAMCGLLPGRTDLLSPVVMVNLLGDVWQADGGEPNWNVLMEAP
                     NAHLHLYGKKHARPGRKMGHYTVLATDVDAALEQARALKET"
BASE COUNT         1854 a         3321 c         3369 g         1687 t
ORIGIN      
        1 ggccgcgaag aacgccgcgc cgccgccgag accgccgaag gcatcattca ggaaaaaacc
       61 cgcgagttca gccaatggct ggccggccgc gacaccgtgc cggtgatccg cgcgctgcaa
      121 gtcgaagccg aacgcgtgcg ccgccacgcg ctggaagccg ccgccaaacg cctgcacaag
      181 ggcgaagcgc cggaagacgt gctggagttt ctctcccgcc agctcaccaa caagctgctg
      241 cacccgccgc tgcaagcgct gaacagccac cacggccagg ccaaggccga gctgaccgac
      301 gcgctggcgc ggctgtacaa cttgcggccg gactagcgac gcggccgccc cgcacgaaat
      361 caaaccaagc gcgcctaaac gtattaaaat tgccggtttg ccgactggcg caccctgggc
      421 gccgctgtcc gtctgtctct gcccgcacac gcgcgggcca cggaaagaac acccgacgat
      481 gaaagcctcg attgccagca aactggatca gctggttgac cggctcgatg aagtgacgcg
      541 cctgctgtcc accgaagaag ccacccgcga catggagtcg tttcgcaagc tcacccgcga
      601 acacgccgaa ctgaccccgg tggtcgagca ataccaggca tttcgccagt gcgaggccga
      661 tatgcgcgcc gccgaagagc tgctggccga cccggacatg cgcgaactgg cgcaagccga
      721 gctggaagac ggcaaagtcc gcctgggcga gctggagctg gcgctgcaaa aactcctgct
      781 gccgcgcgac cccaacgacg aacgcaatat ttttctggaa atccgcgccg gcaccggcgg
      841 cgatgagtcg gcgctgtttg ctgctgattt gttccgcatg tactcgcgct ttgccgaacg
      901 caaccgctgg caggtggaaa tcgtctccgc cagcgaatcc gacctgggcg gctataagga
      961 aatcatcgcc cggctggtgg gctttggcgc gtattccaag ctgaaattcg aatccggcgg
     1021 ccaccgcgtg cagcgcgtgc ccgccaccga aacgcaaggc cgcatccaca cctcggcctg
     1081 caccgtggcg gtgatgccgg aagccgacga agtggaagcg gtcaacatca acccggccga
     1141 actgcgcatc gacaccttcc gcgcctccgg cgccggcggc cagcacatca acaaaaccga
     1201 ctccgccgtg cgcgtcaccc acctgcccac cggcattgtg gtggaatgcc aggacgaccg
     1261 cagccagcac aaaaacaagg cccgcgccct ggccgtgctg gcctcgcgca tcatggacgc
     1321 gcaactgcgc gaacgccagg ccaaagaagc cgccacccgc aaaagcctgg tgggctccgg
     1381 cgaccgctcg gaacgcatcc gcacctataa cttcccgcag ggccgggtga ccgaccaccg
     1441 catcaacctg acgctgtaca aactcgatca ggtgatggac ggcgacctgt ttgaactcac
     1501 cgaggcgctc agcgccgaac accaggccga acaactggcg gcaatggggg agtgaggcga
     1561 gcgcgcccac tgaacaaagc accactcagc acaacgccct ggcgaatccg ccagggcgtt
     1621 gtcttttgcc aagccaaata cggcgcttag ccttgcagct tccaggccag cgtttcgccc
     1681 gcgcgcagcg gcaccagcgg ggcgtcgtcc agataggcca gggtggccgg cacggtccag
     1741 ctttcccggc gcagggtcac cgtgccgctg ttgcgcggca ggcggtagaa gtccgggccg
     1801 ttaaagctgg cgaaggcttc cagtttgtcc agcgcgccca ccgagtcgaa ggcttcggcg
     1861 tacagctcca gcgcggcgtg ggcggtgtac atgccggcgc agccacaggc ggcttctttg
     1921 gtgtggcggg cgtgcggggc gctgtcggtg cccaggaaga acttggccga gccgctggtg
     1981 gcggccttga gcagcgcctg gcgatgggtt tcgcgcttga gcaccggcag gcagtagtgg
     2041 tgcgggcgga tgccgcccac cagcatggcg ttgcggttca tcagcaggtg gtgcgcggtc
     2101 agcgtggcgg ccacgttagc cgatgcgctg gcgacaaatt cggcggcctg ggcggtggtg
     2161 atgtgctcca gcaccacgcg cagctcgggc aggcgggcaa tcagcggggc cagggtgcgt
     2221 tcgatgaaca ccgcttcgcg gtcgaagatg tcgatgtccg ggtcggtgac ttcgccgtgc
     2281 agcagaaagg gcaggccgac ttcggccatt ttttccagca ccggcaggca gtgttccagc
     2341 gagctgacgc ccgaatcgga attggtggtg gcgcccagcg ggtagagttt gacgccgtgc
     2401 acgatgccgc tggccttggc gcggacgatt tcatccggcg tggtgcggtc ggtcaggtac
     2461 agcgtcatca gcggctcgaa agtcatgccg gccggcagcg cggccaggat gcgggcgcgg
     2521 taggcttcgg cgtcggccac ggtggtgacc ggcggcttga ggttgggcat gacgatggcg
     2581 cggccaaact ggcgggcggt gtcgggcagc acggcggtga gcgccgcgcc gtcgcgcagg
     2641 tgcaggtgcc agtcgtctgg gcgggtgagg gtaagcgtgt cggtcatggt cgggctttgc
     2701 gggtgaaaac tgttacatta taacatttat ccgggcgctg gcgcagggcc gcggcggatg
     2761 ctcagcgtcg cttacttgcg gcgcagcacc atgacgaact tgccctcgtc ggtgacgatg
     2821 ctgtcttcca gggtgttgcc ggtttgccgg cagaaagcag caaaatcgcc cggtgcgccg
     2881 gggtcggtgg cggtgacgcg cagcacctgg ccagagctca tgtcagccag ggcttttttg
     2941 gcgcgcagga tgggcagggg gcagttcagc ccggaaaggt cgatgtcttt gtcggcagtc
     3001 atggctcgct ggctcaggta gaatgcaaag accttatttt agcccagcgg ccttgccatg
     3061 tgcctgatcg gaatcgctta ccaacatctg cccggcacgc cgctcttatt gctggccaac
     3121 cgcgatgaat attacgcgcg gccggcacgg cccatcgatc gctgggaaga cgccccgcat
     3181 attctgggcg ggcgcgatgg acaagccggc ggcagttggc tggcggtgag cgaatccggc
     3241 cgagtggcgg cagtcaccaa tgtgcgcacc ggcagccgga cgcccggccg gcgctcgcgc
     3301 ggcgagctgg tgcaagcctg gctgcaagac agccgcccgg cggccgagtt tgccggctgg
     3361 cttcaggcgc agcgcggcga gtatgcgccg tttaatctgc tgtttggctg cctgaacgat
     3421 ttgtattgtt ttcatagccc gcgcgcgctg ctcagccggc tgcgccccgg tgtgcacgtg
     3481 ctgagcaacg gcctgccgga tgcgccgtgg cccaagagcg agcggctgcg cggctggatg
     3541 agcgccatgc acagcttgcc ggccgacgag gtgatgctgg attggctggc cgaccgcacg
     3601 ccggccccgg tagaggccct gcccaatacc ggcgtgggcc tggcgctgga gaaattcctg
     3661 tcgccggtgt ttattgccgg cgacgattac ggcacccgcg ccagcaccgt gctgacactg
     3721 ggcagcaagg ggcgtggcgt gctggtcgag cgggtattcc acgccggccg gccgcaaggc
     3781 tcgcgccggc tgtcgtttgc cgtgcaagcc ggtgcacgcg cgtgctgaac ctgatgattt
     3841 gtttgttgaa ggataccccc gtgaggcgtt tttccctgct ggccgcgctg gccgtcgccg
     3901 ccttgagccc gctggctcag gctgaatacc gcaacgacat gtttaaccag aattacgtct
     3961 gggaaaccga aaaaccctgg gaagaaaaaa ccgtcgagct gccggcctac ccggacggca
     4021 aacactggca agaatttgaa gtcggcccca ccacccgcaa ccgccacgcg gtagacaccg
     4081 ccaccctgcg cctggacgac gacggcgtgg tgcgctttgc cgtgcgcgtg caaagcccgg
     4141 acggcgcgga aacgctgact tacgacggcc tgcgctgcga aacccgcgag cacaagcgct
     4201 acgccattgg cgctgagaat aaaacctggg cgccgtcccg ctcgtcgcag tggcggcggc
     4261 tggagtctgg ccaggcggtg ctgcgcgcgc tggaagagtt ttatctgtgc gtggaccgca
     4321 gcccggtggc cagtgtgaac gaagccatcc ggcgcatgaa gcaacgctaa gcgccgcatc
     4381 agcctcaggc aaacaacgca gcatcacgcg ccagtgcacg ggctgcctcc gcgtcgatca
     4441 cgccttgccc gcacagcgcc aacaaagctt gatccagcgt ttgcatgccc tgcgcctggc
     4501 cagtttgcat cgccgaatac agttgggcag catcaccctc gcgcaggtga tgacgcacgg
     4561 ccgggttagc caacagcagc tcaaacgccg ccacccgcct agcgccatca gccgtcttga
     4621 tcagcgtctg cgccaccacg gcgcgcaggc agtcggccag caccgcgcgc gccatgtcgc
     4681 gttcggcggc gggcaggctg ttgatcaccc ggtctggcgc ttgcgctgcc gactgggtgt
     4741 gcagggtggc cagcaccaga tggccggttt cggcagcggt cagtgccaga cgcatggtgt
     4801 cggcgtcgcg catttcaccc agcaggatca cgtccgggtc ttcgcgcaag gcggcgcgca
     4861 gggccgtggc aaagccatgg gtgtggcggc ccacttcgcg ctggtgcacc acgctgagct
     4921 ggcaggggtg gatgaactcg atcgggtctt cgatggtcag gatgtgagca gcgcgccggg
     4981 cattgatgcg cgccagcagc gccgccagcg tggtggattt gcctgatccc gttgcgccgg
     5041 tcaccagcac caggccatgc ggcaggcgat ccagcgcaca cacggcaggt ggcgcaccca
     5101 ggtgttccag cgctggggcg tgctcgggaa tcaaacgaat ggccgccgcc ggaccacgct
     5161 gctggctgaa tacattcacc cggcaacggc cgctatccgg ccaggccagg gcaaagtcgc
     5221 actcgtgcgc ttcggcatat tggcggcgct ggccttcgtc catcatcgaa tgcagcaagg
     5281 cgcgcacctc ggcggcagac agcgcaggca gggcaatcgg gcggatgtcg ccatgcacgc
     5341 gcaacagtgg cggcaggccg gcggacagat gcaggtcaga ggcgtgctgg ctgcgggcaa
     5401 aggcgagaag atcggggatt tccatttata ctgcaccttt cttcaatggc ctggccaatc
     5461 attcccgacg ttgcggaaca tgatcacaga aagggcgcac caggcttgcg ccccagtgga
     5521 ggggacttta ccatgacaat cgaatcagcg ctgcgcgcca cgcgcgagcg gctggcgcag
     5581 gcagaagccg ccgccggccg tgaggcgggt tcggtgcgcc tgctggcggt gagcaagact
     5641 tttccggccg aggcggtgcg cgaagctgcc gccgctggcc agcagtgctt tggcgaaaat
     5701 tacgtgcagg agctggccgc caaggccgtc gagctggccg aattggggct ggaatggcat
     5761 ttcatcggcc cgctgcaaag caataaaacc cggcctgtag ccgagtgcgc gcactgggtg
     5821 cattccatcg accgcctgaa aatcgccgaa cggctgtcgg cgcagcgccc acaaaacatg
     5881 ccggacttgc aggtttgcct gcaagtgaat gtctcgggcg aggccagcaa aagcggcgtc
     5941 agcccggcag aaatcccggc gctggccgcg gcggtggcgg cgctgccgcg cctgcgcctg
     6001 cgcgggctga tgtgcattcc cgaggccacc gacgacttgc cgcgcctgcg cgaacaattg
     6061 gccgtgctgc cacagctgcg cgcgcagctg gccgaacagg gcctggcgct ggacaccctg
     6121 tcgatgggca tgtccgccga cctggagctg gcggtggcct gtggctcgac cctggtgcgc
     6181 gtgggcaccg ccatttttgg tggccgcacg ccgcgtcacc ctgcctgacc ttcacccatc
     6241 ggaattcatt gtcatgatgc acatcacttt catcggcggc ggcaatatgg ccaccgccat
     6301 catcgccggc ctgaccaagg cccgtggctg ccagatcagc gtggtcgagc ccaatgccga
     6361 caagcgcgca cagttggccc agcagtttgg cgtgaccacg caggagcagc tgccggtgta
     6421 ttttggcagc ggccagattg tggtgctggc ggtcaaaccg cagcagctgc gcgaggtgtg
     6481 cggcgggctg gccagccggc tggatggcgc gctggtgctg tcgattgccg cgggcgtggg
     6541 cgtgtcggcg ctgtcgcgct ggctggatgg ccacgagcgc attgtgcgca tcatgcccaa
     6601 caccccggcg atgatcggca aaggcgtggc cggcctgtac gcgccgcgtg gggtggacga
     6661 gcaagaccgt gacgccgccc agcgcatcat ggcggcagcc ggcagcacgg tgtggctgga
     6721 tgaagaatcc ggcatcgacg acatcacggc gatttccggc agcggcccgg cctatgtgtt
     6781 ttattttatc gaaggcctga tcgacgccgc ccgtcaaatg ggctttgacg acgccaccgc
     6841 ccgcgagctg gcgctgggca ccttcgaggg cgcggtggcg ctggcgcggc annnnnnnnn
     6901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ncacccgctg gcgcagttca ccgtcacgct
     7021 gaccaacttc gcggtgattc cgattcgccg ccttgtgccc agcgtgcgcg gctacgacag
     7081 cgccaccctg ctgctggcct ggctggtgac gctggtttcc acgctggttc tgctgtggct
     7141 gtcgccgttt ccgtataatt ttgccgcgcc gcaaagctgg ctggtgctgg ccttgctggc
     7201 cacactcaag gtatttgtgc tctcgctcta cctgctgatg ggcgcgctgg tggtgcaggc
     7261 cattctgagc tgggtgaacc catacaaccc gctggcgccg attctggaca gcctcacccg
     7321 gccgtatctg cgcccgttcg agcgcctgcg catcggcgca gtggacctgg gcccgctggt
     7381 gttgctgctg attctgcaaa tgctggtggg cattccgctg gcgcggctgg aatggctgct
     7441 gctgagccag atgcaaatgt tgatgtaagc ttgatctgaa tcggcgcccg gcagcagaaa
     7501 atgggagtaa gctgctgggc gcacaaagct gtgtgatctc cctatccacc cctaggagtt
     7561 tgactatcat gaaaaagctg ctcgtttccg ctgctgccct ggctctcttg gcctctgccc
     7621 cggctttcgc caaccaagcc ctggctcaga agaacaactg cctgtcctgt cacgctgtgg
     7681 acaaaaaggt ggtgggcccg gcttacaagg aaatcgccgc caagtacaag ggcgacaaga
     7741 cggctgaagc caagctggtc gaaaaagtga aaaagggcgg cgtgggcgct tggggccaga
     7801 tcccgatgcc ggccaacgcc caggtcagcg acgccgacgt caaggccctg gtgaagtgga
     7861 ttctgtcgat gtaatccccg gcagaatccg tggatgggca gccacagcgc ccatccacac
     7921 agaaaacgtc ctttgctgac gagacacccg catctactct tgtatttgtc tttcctgcca
     7981 gatggcgcaa acgcgccgcc agcggccacg cacggcgccg atgacaggta aaagttgcat
     8041 ttccccggca aactccttaa ccttcctgcc cgtttgccca ccctctcctt agcaggacac
     8101 cgccatggcc aaattgaccg aacaggacat cctgaactac gacggcgacg actacatgaa
     8161 cgccgaccac ctggagtttt tcaaggatcg tctgctgcaa atgcagcaag aactgctgag
     8221 caacgccaac gccaccacca gccatctgca agagcaggaa gccacgccgg atccggctga
     8281 ccgcgccacg ctggaagaag aatacgcgct ggagctgcgc actcgcgacc gcgagcgcaa
     8341 gctgctgctg aaaatccagt ccaccctgaa aaaaatcgac gctggcgact acggctactg
     8401 cgaagacacc ggcgagccga ttggtctgaa gcgcctgctg gcgcgcccga ccgccagcct
     8461 gtcggtcgag gcgcaagagc gccgcgagcg gatgaaaaag cagtacgcag actgacacgg
     8521 ccgtttttcg ccgctgtttt gcgcccaccc tacccacccg gttcagccag ggtggcggtg
     8581 ggcgttgttg cgtctgggcg tcatcctgat ctgacaaact cagccgacgc ccgccatcgc
     8641 aaggatatgc gcagtgtccg caccctggcg gcaaactggg ttaaaatggc ggattgtcca
     8701 tttctgcccg agccttgcga tgattcaagt tggcgtcgtg atggggagca acagcgactg
     8761 ggaagtcatg cagaacgccg cgcgcgtgct gaaagacttt ggcgttgctt ttgaaacccg
     8821 cgttgtttcc gcccaccgca ccccggattt gctgttcgaa tacgccgaaa cggccgaagc
     8881 gcgcggcttg caggcgatta tcgccggcgc cggcggcgcc gcccacctgc cgggcatgct
     8941 ggcggccaaa acccacattc cggtgctggg cgtgccggtg ccgtccaaat acctgcgcgg
     9001 cgaagattcc ctgctgtcca tcgtgcaaat gcccaagggc attccggtgg ccaccttcgc
     9061 cattggcgag gccggcgccg ccaacgccgc gctgtttgcc gtggccatgc tggccaacca
     9121 atcgccggaa ctggccgccg cgctgaaggc cttccgccag aagcaggccg acaccgtgct
     9181 ggcgatgacc ctgccggagg tgctgtaatg caggcgattc tgccgccggc catgctgggc
     9241 attctgggcg gtggccagct gggccgcatg tttgtcactg ccgccaagcg catgggttat
     9301 cgggtgaccg tgctcgaccc cgacgcgcaa gcgccagcgg cggagtttgc cgatgtgcat
     9361 ctgtgcgccg cctacaacga cgctgaggcg ctgcgcaccc tggccgccag ctgcgcggcc
     9421 atcaccaccg aattcgaaaa cgtcaacgcc gacgccatgc gcgagctggc caaaaccacc
     9481 cgcgtgtcgc cgtctggcga ctgcgtggcg attgcgcaag accgcatcgc cgaaaaagcc
     9541 tgggtgaaca aggccggcct gcccaccgcg ccgtatctgg ccatcgaaag cgtggaagac
     9601 atccaggtgg agctcgcccc ctacctgccc ggcatcctga aaaccgcccg gctgggttac
     9661 gacggcaagg gccaggtgcg ggtgaacagc gccgacgaag cccgcgccgc ctacgccaat
     9721 ctgggcggcc aggcctgtgt gctggaaaaa atgctcgacc tcaagctgga agtctcggcc
     9781 atcgtcaccc gcgtgtcgcc cgagcagctc agcgtgtttc cggtggcgga aaacatccac
     9841 aaaaacggca ttctggacga atccatcgtg ccggcgcgca tcgcgccggc cctggccgcg
     9901 caagcgcagc agatggcccg ccaactggcc gaggcgctgg attacgtggg cgtgctggcg
     9961 gtggagtttt ttgtgctggc cgacgacagc ctggtgatca acgaaatcgc cccgcgcccg
    10021 cacaactccg gccactacac catcgacgcc tgcgtgagcg accaatacca gcagcaagtg
    10081 cgcgccatgt gcggcctgct gcccggccgc acggatctgc tgtcgccagt ggtgatggtg
    10141 aacctgctgg gcgacgtctg gcaggccgac ggcggcgagc ccaactggaa cgtgctgatg
    10201 gaagcgccca acgcccacct gcatctgtac ggcaaaaagc acgcacgccc cggccgcaag
    10261 atgggccact acaccgtgct ggccacagac gtggacgccg cgctggaaca ggcgcgcgcc
    10321 ttgaaggaaa c
//
LOCUS       DAOPYX010000078        10305 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_7543_length_10305_cov_10.132098, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000078 DAOPYX010000000
VERSION     DAOPYX010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 10305)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10305)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10305
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7543_length_10305_cov_10.132098"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(107..598)
                     /locus_tag="PKN55_05640"
     CDS             complement(107..598)
                     /locus_tag="PKN55_05640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008953270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNX70755.1"
                     /translation="MQIVKNTVVTLHYEMYDSENTLVDKTEADEPISYLHGGYDGIFP
                     LVEEKLHEKKAGDKVDVLMQPDDAFGDYEAELVRVEPLDVFPDDVELTVGMVFEADDP
                     ETGDVLMFRVTQIADGKVVVDANHPLAGQSVRFVATVADVRPASPDEISHGHAHGEHG
                     HHH"
     gene            725..1846
                     /locus_tag="PKN55_05645"
     CDS             725..1846
                     /locus_tag="PKN55_05645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011137128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNX70756.1"
                     /translation="MNKAIDILGGMDAETFLRDYWQKAPLLIRQAIPDVGQPVDFAWL
                     SELASRDDVESRLIEYRQDRWKVEHGPLSARRLAKLPDSDWTILVQSVNHHLPHIADI
                     LWRFDFLPYARLDDLMISYAPPGGTVGPHFDSYDVFLLQVGGRKRWQISGQEDVSLVD
                     GAPLKILQRFDADQTWELGHGDMLYLPPKYAHYGVALEPGMTYSIGFRAPTRQEMVTQ
                     FLVYLQDRLCCDGMYADPNLAPIAEPGRIGADMVEQVSAMLQAIQWDRDTVTDFLGHY
                     LTEPKAHVFYDAPEEELDEDEFAQALAENGLRLDLKSQILFTESGVYANGERLEIAES
                     DRPHWQALANRRRLAAGVYPDTLTEALYDGYLHGFWHVH"
     gene            complement(2742..3875)
                     /gene="bamC"
                     /locus_tag="PKN55_05650"
     CDS             complement(2742..3875)
                     /gene="bamC"
                     /locus_tag="PKN55_05650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008953268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HNX70757.1"
                     /translation="MNFRHASLILSASLLASACSSTGLFDKKIDYKSAENAAKNRLEV
                     PPDLTRPQLNNNYALPGASSVSAQQLNQQAQANLLNKNPSNVLIDIPVARMERAGQQR
                     WLVVQKKPEELWPLLKEFWIDNGFILKQDAPEIGVMETDWAENRAKIPQDGIRNLLSK
                     VGLDNVYSTPERDKFRARIERTNAGTEVYFSHRGMYETFVNEGKAETMWQPRPADPEL
                     EAEMLGRFMMRMGISEEKVKLAAQKPDANQPQRASVQGEAVLVNDAFDRAWRRTGLAL
                     DRIGLLVTDRDRSQGVYYIRPAQAEMDKSDESGGFWSSLLFWRSSDKPASAKAPLAGG
                     DYQVRVKAVNDSSSQVLVLDKNGQALPANVSRPMLARLQQQLQ"
     gene            complement(3894..4769)
                     /gene="dapA"
                     /locus_tag="PKN55_05655"
     CDS             complement(3894..4769)
                     /gene="dapA"
                     /locus_tag="PKN55_05655"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012696182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HNX70758.1"
                     /translation="MLTGSLVAIVTPMAEDGSVDYAALDRLVDFHIENGTSGIVAVGT
                     TGESATLAVDEHLKVVESVVKRANKRVAVIAGTGANSTREAIELTEAAASLGVDSALS
                     VVPYYNRPTQEGMYQHFKAIAEACPLPLILYNVPGRTVADMSNDTALRLAEIPNIIGI
                     KDATGNLERACDLVRRAPADFALYTGDDASAMAFMLCGGHGVISVTANVAPKLMSELC
                     RAALAGDCLGARAINDQLQGLHKQLFIEANPIPVKWAMERMGYIPSGIRLPLTRLSEA
                     CQPVVEAAMRQAQAL"
     gene            5161..6765
                     /locus_tag="PKN55_05660"
     CDS             5161..6765
                     /locus_tag="PKN55_05660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002641409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HNX70759.1"
                     /translation="MITTSNITMQFGAKPLFEKVSVKFGDGNRYGLIGANGCGKSTFM
                     KILGGDLEPTQGTVSIENGLRLGKLRQDQFAYEEQRVIDVVLQGHVEMWAAMSERDAI
                     YANLEATEDDYMRAAELEGKFAEYDGYTAEARAGELLMGVGIPVEQHFGPMSEVAPGW
                     KLRVLLAQALFSNPDILLLDEPTNNLDINTIRWLENTLNQRNSTMIIISHDRHFLNSV
                     CTHMADVDYGEIRIYPGNYDDYMLASAQARERLLSSNAKAKERIGELQDFVARFSANK
                     SKARQATSRLKQVDKLKAEMVEVKPSSRQNPYIRFEMEDKDKLHRQAVDISGVSKSYD
                     KPLIHNLNLILDAGQRLAIIGPNGSGKTTLMKMLVNELQPDQGRIKWAEKANPGYFAQ
                     DHEADFDLDYDLAEWMRQWTQEGDDEQVVRGTLGRLLFGGDDVKKNVRVLSGGEKGRM
                     LYGKLILQKPNVLLMDEPTNHMDMESIESLNMALEKYKGTLIFVSHDREFVSSLATQI
                     LELDGKGGYDYYTGGYEDYLASKGIE"
     assembly_gap    6839..6938
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            7221..7961
                     /locus_tag="PKN55_05665"
     CDS             7221..7961
                     /locus_tag="PKN55_05665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017510080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP reductase"
                     /protein_id="HNX70760.1"
                     /translation="MRLKGKVSIITGGASGIGKATVLKFAQEGAIVAVCDLNQETIDA
                     TVNEVKAAGGEAVGYIVNVTNKSQINDMVADVKARFGRIDVLVNNAGIVQDAQLTKMT
                     DEQFDLVIDINLRGVYNCAKAVVDTMVAQGGGVILNASSVVGVYGNFGQTNYAATKFG
                     VIGFVKTWAKELGKKGIRANAVCPGFVATPILKAMPEKVIQAMEDRVPMKRMAQPEEI
                     ANLYAFLASDEASYINGAAIEITGGLTL"
     gene            8369..8932
                     /locus_tag="PKN55_05670"
     CDS             8369..8932
                     /locus_tag="PKN55_05670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011135454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HNX70761.1"
                     /translation="MPAADTALLLIDVQQSFTVRPYWDAQDVPAFLAACNTLIAGCLK
                     RHVPIVRVFHVEDDGAFALASGLVRPLDGLLAFAEQARIDKHVHNAFTDTGLDRWLRG
                     RGIRRVLIAGIRSEQCCETSARVASDLGYEVDFVSPATLTFAMRHPLSGAHYSAADIR
                     ARTELVLAERFAQVCSVEQALARLDAD"
     gene            9030..9575
                     /locus_tag="PKN55_05675"
     CDS             9030..9575
                     /locus_tag="PKN55_05675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011136704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HNX70762.1"
                     /translation="MRATLFCPALVALCLSAPALADKFTLSSPDLHAGQRMADTFVFK
                     GFGCEGGNQSPALRWSAPPANTRSFAITVYDPDAPTGSGWWHWQVVNLPASVRELPRN
                     ASADGLPAGAVQTRTDFGKPGYGGACPPPGHGKHRYQFTVWALDVASLPLDGEASGAL
                     TGYMLRQHALGQAQLTVHYSR"
     gene            9572..>10305
                     /locus_tag="PKN55_05680"
     CDS             9572..>10305
                     /locus_tag="PKN55_05680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX70763.1"
                     /translation="MNARASLHVPGVASWIEFRAAREQRVRAVRFPRLTLVRVREGAK
                     EICHGGQRMLAHAATLLLAPAGSVMDVCNQPGAGCFRSECLTLDPTLVERFAVREASL
                     LHSAPPPPCVAVTPMLAEVWAHAMHGWETPTDARVLAHRLEELLLALALAGHGAALRP
                     AASLAVSERVRRLLLAKPGADWRADQVAAQLHCSAATLRRQLARERAAFSDILRECRL
                     GQALMLLQTSPWPVARIAAECGYDCAS"
BASE COUNT         1954 a         3177 c         3103 g         1971 t
ORIGIN      
        1 ctagccagga tcgctgcgct gggttttgtg agcggctcta tgcaaacagc cgccatacgg
       61 cggctgtttg cttcatcgcg cgattccgtg acggaatcag ccaacatcaa tgatgatggc
      121 cgtgttcgcc gtgggcgtga ccatgcgaga tttcgtccgg gctggccggg cgcacatcgg
      181 ccacggtggc gacaaagcgc accgattgac cggccagcgg atggttggcg tccaccacca
      241 ctttgccgtc ggcaatctgg gtgacgcgga acatcagcac atcgccggtt tccgggtcgt
      301 cagcttcgaa caccatgccg acggtcagtt caacgtcgtc ggggaacacg tccagcggct
      361 ccacgcgcac cagttcggct tcgtagtcgc caaaggcatc gtccggctgc atcagcacgt
      421 ccactttgtc gccggctttc ttctcgtgga gtttttcttc caccagcggg aaaataccgt
      481 cgtagccgcc gtgcaggtag ctgatcggct cgtcggcttc ggttttgtcc acgagcgtgt
      541 tttcggagtc gtacatttcg tagtgcagcg tcaccacggt atttttaacg atttgcattg
      601 aagaaccttc ttgaagaaat ttgcggggag ttccccgccg gacagcgccc tgctgggtgg
      661 ctgcatggtt cgcattgtaa accattcagg agcgcctcgc cgccacgcgg tcgggcgaca
      721 aagcatgaac aaagcaattg acatccttgg gggcatggac gcagaaacct ttctgcgtga
      781 ctactggcaa aaggcccccc tgctgatccg ccaggcgatt cccgatgtgg gccagccggt
      841 ggattttgcc tggctaagcg agctggccag ccgcgacgat gtggaatcgc ggctgatcga
      901 ataccggcaa gaccgctgga aagtcgagca cggcccgctg tcggcgcgcc ggctggccaa
      961 gctgccggac agcgactgga ccattctggt gcaaagcgtc aaccaccacc tgccgcatat
     1021 tgccgatatt ttgtggcggt ttgatttcct gccttacgcc cggctggatg atttgatgat
     1081 cagctacgcg ccgccaggcg gcacggtggg gccgcatttt gattcttatg atgtgttttt
     1141 gctgcaagtg ggcgggcgca agcgctggca gatttccggc caggaagacg tctcgctggt
     1201 ggacggcgcg ccgctgaaga ttttgcagcg ctttgacgcc gaccaaacct gggaacttgg
     1261 gcacggcgat atgctttatc tgccgcccaa gtacgcacac tacggcgtgg cgctggagcc
     1321 gggcatgacc tactcgatcg gctttcgcgc gcccacccgc caggaaatgg tcacccagtt
     1381 tctggtgtat ttgcaggaca gattgtgctg cgacggcatg tacgccgacc cgaatctggc
     1441 gccgattgcc gagcccggcc gcatcggtgc ggacatggtc gagcaagtca gcgctatgtt
     1501 gcaggccatt cagtgggacc gcgacacggt aacggatttt ctcggccatt atctgaccga
     1561 gccgaaggcg catgtgtttt acgatgcgcc ggaagaagaa ctggacgagg atgagttcgc
     1621 ccaggcgctg gcagaaaacg gcctgcgcct ggacctgaaa agccagattc tgttcaccga
     1681 gagcggggtg tacgccaacg gtgagcgcct ggaaatcgcc gagtccgacc gcccccattg
     1741 gcaggcgctg gccaatcggc gccggctggc cgccggggtg tatccggaca cgctgaccga
     1801 ggcgctgtac gacggttatc tgcatggttt ctggcacgtg cactgaactg ttgcatgagc
     1861 gcgacagaac atgttgcgct gcatcaatat gctttcatat tcggaaaaga tgtgacttaa
     1921 tagaaccaag gcagtgcaca acgcattgtg gtgtgcgcct gagaagtgca tgcgcggtaa
     1981 agtccgcctt ttttcggctt gcatcttttc cgagcttttt ttcgtgctac aatcttgcag
     2041 tcggtcgggg tcgtcatccg ctgagctttc tgccccgacg tgttttgtcc aacgctactt
     2101 ttattcgaag gtactgataa tgaaacaatc gctcctcgtc gctgccctgc tggccgttgc
     2161 cctgtccgct tgcggcaaaa aagaagaagc cgctccggct ccggccccgg ctcctgaagc
     2221 tgctgccccg gccgctgctc cggctccggc tccggctgct ccggccgctg acgctgctgc
     2281 cccggccgct ccggctgccc cggccgctga cgctgctgcc ccggctgctc cggctgccgc
     2341 tccggccgaa gaaaagaagc agtaattcct gcttcgcgct gaatgcgcaa aaagccgacc
     2401 tccgggtcgg ctttttttat gcggattgtg tagccaggca tttaatgcct gccgcatccg
     2461 gctaaaaatt gcccatcccc tgttgtactt cccatctggc atgcgcacaa acgcaagacg
     2521 ccacacggcg gacatgcgaa gttgaacatc gccagccagt cagcgcctcg cccgcacgct
     2581 catcaccaca cgtccccccg ccacacggtg gtgcgcacca gaaaagtgca atgcaccatt
     2641 cgtcccgcgc ccacacagtc tagccgtggc tgaaaaacaa cagggcgtcc cagatggaac
     2701 gccctgtggc agaacagata cacgcctgac ggcgtggcag cttactgcaa ttgttgctgc
     2761 aagcgggcca acatcggccg gctgacattg gccggcagtg cctggccatt tttgtccagc
     2821 accagcactt ggctgctgct gtcattcacc gctttcaccc gaacctgata gtcgccaccc
     2881 gccagcggcg ccttggccga ggccggcttg tcgctgctgc gccagaacag caggctggac
     2941 cagaagccac cagactcgtc gctcttgtcc atttcggcct gtgccggacg aatgtagtac
     3001 acgccttgcg aacggtcacg atcggtcacc aacagaccaa tgcgatccag cgccaggcca
     3061 gtacgacgcc aggcacgatc aaaggcatcg ttcaccagca cggcttcgcc ttgcacgctg
     3121 gcgcgctgcg gctggttggc atccggtttc tgggccgcca gcttgacctt ctcttccgag
     3181 atgcccatgc gcatcatgaa acgaccgagc atttcggctt ccagctcggg gtcggccggg
     3241 cgcggttgcc acatggtttc tgccttgcct tcattgacga aggtttcata catgccgcga
     3301 tggctgaaat acacctcggt gccggcattg gtgcgctcga tgcgggcgcg gaatttatcc
     3361 cgctccggcg tcgagtagac attgtccagg cccaccttgc tcagcaggtt gcggatgcca
     3421 tcctgcggaa tcttggcgcg gttttccgcc cagtcggttt ccatcacgcc gatttccggt
     3481 gcatcctgtt tgaggatgaa gccgttgtcg atccagaact ctttcagcag cggccagagt
     3541 tcttccggct ttttctgcac caccagccag cgctgctggc cggcgcgctc catacgcgcc
     3601 accgggatgt cgatcagcac attggacggg tttttattga gcagattggc ttgcgcctgc
     3661 tggttgagct gctgggcgct caccgagctg gcgcccggca gcgcgtagtt gttgttcagc
     3721 tgcggccggg tcagatccgg cggcacctcc agccgattct tggcggcgtt ctcggcggac
     3781 ttgtagtcga ttttcttgtc gaacaagccg gtgctgctgc acgcgctggc aagcagcgag
     3841 gcggagagga tcaagctggc gtggcgaaaa ttcatcacgg ctccgtgaac gagttaaagg
     3901 gcctgggcct ggcgcatggc ggcttccacc accggctggc aagcctcgga caggcgggtc
     3961 agcggcagac ggatgccaga gggaatatac cccatgcgct ccatggccca cttgaccgga
     4021 atcgggttgg cttcgatgaa cagctgcttg tgcaggcctt gcagctgatc attgatggcg
     4081 cgcgcgccca ggcaatcgcc agccagcgcc gcgcggcaca gctcgctcat cagcttgggc
     4141 gccacgttgg cggtgaccga aatcacgccg tgaccgccgc acagcataaa ggccatggcg
     4201 ctggcgtcgt cgccagtgta caaggcaaaa tcggccggcg cgcggcgcac cagatcgcaa
     4261 gcgcgctcca gattgccggt ggcgtccttg atgccgatga tattggggat ttccgccaga
     4321 cgcagcgcgg tgtcgttgga catatccgcc acagtgcgac ctggcacatt gtacagaatc
     4381 agcggcagcg ggcaggcctc ggcaatcgcc ttgaagtgct gatacatgcc ttcttgcgtc
     4441 gggcggttgt aataaggcac aaccgacagc gccgaatcca cgcccaggct ggcggcggct
     4501 tcggtcagct caatggcttc acgggtggag ttggcgcccg ttccggcaat caccgccacc
     4561 cgtttgttgg cgcgcttgac caccgactcg accactttca ggtgctcgtc caccgccagg
     4621 gtggccgatt cgcccgtggt gccgacggcg acgatgccgc tggtgccgtt ttcgatgtgg
     4681 aaatccacca ggcggtcaag cgcagcgtaa tcgacgctgc cgtcttcagc catgggggtg
     4741 acaatcgcga ccagacttcc ggtaagcata ctgtttgcct atttgacggg atacaaagca
     4801 caggattgta gcccaatcac cgttcatggc aaaccgccgc aatcaggccg ggatggaatg
     4861 agttttgact gcatgacggc aagcgagtgc aacatcgctg gattctctgc gccaccgggt
     4921 ggcatctgcg agtcaaaacc cgataggcag gccccggctg tgtggcattc gatacatctg
     4981 gcgcccgcca gtcttgagat cagcgcagct gacgggcatc tgtctgccca aggtttgctc
     5041 atgaacaggg gaaaccttgc cctagatgcc gcctcatgca cagcgcaggc ccacccgcgc
     5101 cggctgtgct aaaatgatcc gtttttctgt actcaacaga ttgttcagca aggatttcca
     5161 gtgattacca cttccaacat caccatgcag tttggcgcca agccgctgtt cgagaaggtg
     5221 tcggtcaagt tcggcgacgg caaccgctat ggcctgatcg gcgccaacgg ctgcggcaag
     5281 tccaccttca tgaagatcct cggcggtgac cttgagccca cccagggcac ggtgtccatc
     5341 gaaaacggcc tgcgcctggg caagctgcgc caggatcaat tcgcctacga agaacagcgc
     5401 gtcattgatg tggtgttgca aggtcatgtc gagatgtggg cggcgatgag cgagcgcgac
     5461 gcgatttacg ccaacctgga agccaccgaa gacgactaca tgcgcgcggc cgagctggaa
     5521 ggcaaatttg ccgaatacga tggctacacc gccgaggcgc gcgctggtga gctgctgatg
     5581 ggcgtgggca ttccggtgga acaacacttt ggcccgatga gcgaagtggc ccccggctgg
     5641 aagctgcgcg tgctgctggc gcaggcgctg ttttccaacc cggacatcct gctgctggac
     5701 gaaccgacca acaacctgga catcaacacc atccgctggc tggagaacac gctgaaccag
     5761 cgcaactcca ccatgatcat catctcgcac gaccgccact ttttgaactc ggtgtgcacc
     5821 catatggccg acgtggatta cggtgaaatc cgcatctatc cgggcaacta cgatgattac
     5881 atgctggcct cggcgcaggc gcgcgagcgt ttgctgtcgt ccaacgccaa ggccaaggag
     5941 cgcattggcg agttgcagga ctttgtcgcc cgcttctcgg ccaataaatc caaggcccgc
     6001 caggccacca gccgcctgaa gcaggtggac aagctcaagg ccgagatggt cgaggtcaag
     6061 ccgtcgtcgc ggcaaaaccc gtacatccgc tttgaaatgg aagacaaaga caagctgcac
     6121 cgtcaggcgg tggacatcag cggcgtgagc aagtcttacg acaagccgct gattcataat
     6181 ctcaacctga ttctggacgc cggccagcgc ctggccatca tcggccccaa cggctcgggc
     6241 aagaccacgc tgatgaaaat gctggtgaac gagctgcagc cggatcaagg ccgcatcaag
     6301 tgggcagaaa aagccaaccc gggctatttt gcccaggatc acgaggccga ttttgacctg
     6361 gattatgatc tggccgaatg gatgcgccag tggacgcaag aaggcgacga cgaacaagtg
     6421 gtgcgtggca cgctgggccg cctgctgttt ggcggcgacg acgtgaagaa aaacgtgcgc
     6481 gtgctgtccg gcggcgaaaa gggccgcatg ctgtacggca agctgattct gcaaaagccg
     6541 aatgtgctgc tgatggacga accgaccaac catatggata tggagtcgat cgaatcgctg
     6601 aacatggcgc tggaaaagta caagggcacg ctgatttttg tctcgcacga ccgtgagttc
     6661 gtcagctcgc tggctaccca gattctggag ctggacggca agggcggcta cgactactac
     6721 accggcggct acgaggacta tctggccagc aagggcattg aataagcaaa cgcccactgg
     6781 gcttgaccgc ctggcacacg gcccgcgacc ggcaacggcg cgggccgtgt tgttttggnn
     6841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnac gcaagcgtca atataagtga
     6961 atcggatttt agagttaacg ctttacccct gcgtaaggct gcggtatatt gcagccattc
     7021 ttcgggccac gctgcccggt agaggagaaa ccaaccgcaa gatagcggct gcaacacaac
     7081 aaaaacatat aacaacgata ataacgaccc aaaatacagc acagctgaag gtcgatggtg
     7141 gagtgagagc gggtccggcc cttcctgatg gaaggccgga catgctttat cccaacccct
     7201 ctagatttaa ggatttcgcc atgcgtttga aaggtaaagt ttcgatcatc accggcggcg
     7261 ccagcggcat tggcaaagct accgttctga agtttgcaca agaaggcgcc atcgtcgccg
     7321 tgtgtgacct gaaccaggaa acgatcgacg ccaccgtcaa cgaagtcaag gccgccggcg
     7381 gcgaagccgt gggttacatc gtcaacgtca ccaacaagtc gcaaatcaac gacatggtcg
     7441 ctgacgtcaa ggcccgcttt ggccgcatcg acgtgctggt gaacaacgct ggcatcgtgc
     7501 aagacgccca gctgaccaag atgaccgacg agcagttcga cctggtgatc gacatcaacc
     7561 tgcgtggcgt gtacaactgc gccaaggccg tggttgacac catggtggcc caaggcggcg
     7621 gcgtgatcct gaacgcttcc tccgtggtgg gtgtgtatgg caacttcggc cagaccaact
     7681 acgccgccac caagtttggc gtgatcggtt tcgtcaagac ctgggccaag gaactgggca
     7741 agaagggcat ccgcgccaac gccgtgtgcc cgggctttgt cgccaccccg atcctgaagg
     7801 ccatgccgga aaaagtcatc caggccatgg aagaccgcgt gccgatgaag cgcatggccc
     7861 agccggaaga aatcgccaac ctgtacgcct tcctggcctc cgacgaagcc agctacatca
     7921 acggcgcagc gatcgaaatc actggcggcc tgaccctgta atcctcgccg attactggtc
     7981 tcgcaaaaaa accccttgtg tcgccacaag gggttttttg ttttttccgc ccaccaaacc
     8041 ccatgcatgg caagcaccgc gcacaacact cagcacaatg cacacgaccg cagaccgtac
     8101 caatttagcg ccgctcacaa aaccctctgg cattgtggtg tgactttgcc gtactgccgg
     8161 tactgtctgc ggtcgtgcgc ctggccaggg ccacctcgct gggttttgta agcgactctc
     8221 atggcaaacc cgtaacacaa agtcatggtt gcttgcgtgc ggtgataaat gtccgttttg
     8281 gtccgcatcc tgtcccgccc cggcgccgac acagtcactc agccctttta cactgtcgtc
     8341 atctcctgtc cacccggaat gtgcgaccat gcctgctgcc gacaccgccc tgctattgat
     8401 tgacgtacaa cagtcgttta ctgtccgccc ctattgggac gcgcaagatg tgccggcttt
     8461 tctggccgcg tgcaatacgc tgattgctgg ctgcctgaag cgccatgtgc caattgtgcg
     8521 ggtgtttcat gtggaagacg acggcgcctt tgccctggcc agtgggctgg tgcgcccact
     8581 ggatggattg ctggcgtttg ccgagcaggc gcgcatcgac aaacacgtgc acaacgcctt
     8641 taccgacacc gggctggacc gctggctgcg cgggcgcggc atccgccggg tattgattgc
     8701 cggcatccgc agtgagcaat gttgcgaaac cagcgcgcgg gtggcgtcgg atttgggcta
     8761 tgaagtcgat tttgtcagcc cggccacgct gacttttgcc atgcgccatc cgctcagcgg
     8821 cgcacactac agcgccgccg acatccgcgc gcgcaccgag ctggtgttgg ccgagcgctt
     8881 tgcccaagtg tgcagcgtcg agcaggcgct ggccaggctg gatgcagatt gagcgccgcc
     8941 gcacactgcc tgcgcgctga cgcactgcgc catgcgctgg cgcaggcgat actgatggct
     9001 ccttcacccg aatagatcag gagattttca tgcgagccac ccttttttgc ccggcgctgg
     9061 tcgccctgtg cctgagcgcc ccggcgctgg cggataaatt taccctgagc agcccggatc
     9121 tccacgccgg ccagcgcatg gctgatacgt ttgtgttcaa gggctttggc tgcgaaggcg
     9181 gcaaccagtc gcccgcgctg cgctggagcg cgccgccggc caacacccgc agctttgcca
     9241 tcaccgtgta cgacccggac gcgcccaccg gcagcggctg gtggcactgg caagtggtca
     9301 acctgccggc cagcgtgcgc gagctgccgc gcaacgccag cgccgacggc ctgccggccg
     9361 gggccgtgca aacccgcacc gactttggca aacccggcta cggcggcgcc tgcccgccgc
     9421 cgggccacgg caagcaccgc tatcagttca ccgtctgggc gctggacgtg gccagcctgc
     9481 cgctggatgg cgaagccagc ggcgcgctga ccggctatat gctgcgccag catgcgctgg
     9541 gccaggcgca actgaccgtg cattacagcc gatgaacgcg cgcgccagcc tgcatgtgcc
     9601 tggcgtggcc agctggatcg agtttcgcgc cgcacgcgag caacgcgtgc gtgcggtgcg
     9661 ctttccccgg ctgaccttgg tgcgggtgcg cgaaggcgcc aaagaaatct gccacggcgg
     9721 ccagcgcatg ctggcgcacg ccgccacctt gctgctggcc ccggccggta gcgtgatgga
     9781 tgtatgcaat cagccagggg cggggtgttt tcgttcggaa tgcctgacgc tggacccaac
     9841 cctggtggag cgctttgccg tgcgcgaagc cagcctgctg catagcgcgc cgccgccgcc
     9901 ctgtgtggcg gtgacgccga tgctggctga agtgtgggcg cacgccatgc acggttggga
     9961 gacgcccacc gacgcgcgcg tgctggcgca ccggctggaa gagctgctgc tggcgctggc
    10021 cttggccggc cacggcgccg ccttgcgccc agcggccagc ctggcggtga gcgagcgggt
    10081 gcgccgcctg ctgctggcca aacctggcgc cgactggcgc gccgaccagg tggccgccca
    10141 gctgcattgc agcgccgcca ccttgcgccg gcaattggca cgcgagcgcg ccgccttcag
    10201 cgacatcctg cgcgagtgcc ggctgggcca ggcgctgatg ctgctgcaaa ccagcccctg
    10261 gccggtggcg cggattgccg ccgaatgcgg ctatgactgc gcctc
//
LOCUS       DAOPYX010000079        10273 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_7588_length_10273_cov_9.133784, whole genome shotgun sequence.
ACCESSION   DAOPYX010000079 DAOPYX010000000
VERSION     DAOPYX010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 10273)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10273)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10273
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7588_length_10273_cov_9.133784"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(116..1003)
                     /locus_tag="PKN55_05685"
     CDS             complement(116..1003)
                     /locus_tag="PKN55_05685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014087678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HNX70764.1"
                     /translation="MSHPLRIAFAGIGLMGLPMCRRLLAAGHPLTVWNRNPAKTAPLQ
                     AAGARHAATLAELAADADIVCLCLSDTAAVEQVMFAADGLAAQLSANTLVVDFSSIEP
                     AATRAFAQRLAEQCGARWVDAPVSGGVVGAEQGRLVIMAGGEPADIERLRAPLAALSQ
                     RLTHMGPVGCGQVTKVCNQLIVAANSLLIAEAVALAERGGVDASLLAPALAGGFADSL
                     PMQILAPRMAARQHEPVQWKVATLLKDLDNAVKLARETDSASPLAALADQLMRLHATR
                     GHGQADLSSVIGLFAGRDA"
     gene            complement(1112..1924)
                     /locus_tag="PKN55_05690"
     CDS             complement(1112..1924)
                     /locus_tag="PKN55_05690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008953578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNX70765.1"
                     /translation="MLEREYLNICLAEPSSLQANIIVSMLNKLGIDNVQVTESGQATL
                     DLLSSPMPPDVVISALYLPDMTGTDLVYRMRDDARMRETPFLLVSSETKRSYLDPIRQ
                     AGTLAILPKPFNSEQLSHALNNTVEFLNAHENRMDSSVLDVAELSVLLVDDSLTARTH
                     IRSVLQRIGFERISEAINGQEAVPLLDTTLFDLVVTDYNMPEMDGKQLVEYVRQHSMQ
                     PSVPILMVSSEHDEGRLAAIEQAGVSAICDKPFEVELIRKLLRNFFVEPSPC"
     gene            complement(2094..3878)
                     /locus_tag="PKN55_05695"
     CDS             complement(2094..3878)
                     /locus_tag="PKN55_05695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012697167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNX70766.1"
                     /translation="MSQSAFEALGLDARIVSTLTQNQLTTPTPVQEQTIPLLLQGRDL
                     IGSAQTGTGKTAAFLLPALHRLAQTPGEKGRGARVLVLTPTRELAQQVARAAETFSKG
                     LQRMKTVCVVGGVSYRIQNDLLAKPHEILVATPGRLIDHVNARRIDFSRLEMLVLDEA
                     DRMLDMGFSDDVLAIANQLPKERQTAFFTATMTHNVRTFASQLLNDPATVEVAPQTAR
                     HEQIDQMLCYVDDLGHKKRLLKHFLAQEGIKQAIIFTATKRDTEELADELCVEGLRAA
                     ALHGDMPQRERTRTLTQLRRGEFDLLVATDVAARGIDVAGISHVFNFDLPRFAEDYVH
                     RIGRTGRAGATGTAIALVGRDDVMPLRRIERFTSHKIRVHEVEGMESRFRPQDRKPGA
                     GGGRPHGGKRPNGGGGWGGKKSFGDRDHQPREHRGDRSFNDRGGDRQFGDRGGMGANA
                     RSEGAAGEHVANRGHFGDRQPRHEGRDNRDGRPFVQRDRQPFAGRQDRAPRPQGEGQQ
                     AREPRARSPYDERQPSDNSNYNPFPNAGRRDGRSFAGGDRRQGPAGQGQGHGGKSHGH
                     GGSQERRPREGGFAAGAGRRDRYPSFRD"
     gene            complement(4161..4583)
                     /gene="arfB"
                     /locus_tag="PKN55_05700"
     CDS             complement(4161..4583)
                     /gene="arfB"
                     /locus_tag="PKN55_05700"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF006718.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HNX70767.1"
                     /translation="MIPITRSLALADDEIEAAFTRAQGPGGQHVNTTDSAVQLRFDVR
                     RSPSLPEPVKARLCALAGRRMTQDGVLLIQSQAQRSQALNRADALAKLVALIQAACQA
                     PRPRKATRPTRASQTRRLDGKAKRGAVKQLRQRKPGHE"
     gene            4720..6360
                     /locus_tag="PKN55_05705"
     CDS             4720..6360
                     /locus_tag="PKN55_05705"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNX70768.1"
                     /translation="MFSWFLRMSIRWKLQLGFFVVTMITTIFNRLLATHELSKMIEIA
                     RADQVPAAVLAQMMDNRSTYIFNSFWESGLEFMVQFMVIGFVARQLVRPIQELRDAMQ
                     AMSRGDLVHRLQETARDELGELQASFNLMRRRFADILREIENSGKQMHQSAFQVTTIA
                     REIAEVSRKEESRSVEVHQVTRSLSDIAHQVEQRAQAAIEQSTLLENRGLEGIDSVRR
                     NIQMMDETATGVAAASTSIGELEAESARIHAIIDTIHDIAGQTNLLALNAAIEAARAG
                     ELGRGFAVVADEVRKLAERSSASAQEVANIIQGLGVRVREVTGSMQNVVEQVADGRQV
                     ANRTVEVIEGMVQEISVAAEGSRAIGEGSQTQVAELGRLQHTLEALFATLHESGSKVT
                     ATAAIGETIFEVSERLNQTMGGFNFRRELQTSRTTEEKRRFPRAENSLRVHVVHEERA
                     FEGISLDISLSGLRLSLGQDQILPSQALLGLKLYMPRSSLEEFRNQQPISLSGRVMWL
                     RKDGEHTLYGIQFENLNEASRQALRQAVTYFNKAPEYQ"
     gene            complement(6487..6849)
                     /locus_tag="PKN55_05710"
     CDS             complement(6487..6849)
                     /locus_tag="PKN55_05710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012697958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mth938-like domain-containing protein"
                     /protein_id="HNX70769.1"
                     /translation="MKLHLHTSPLNLVTQYGDGYLDVNQTRYTENVLITPEHVAPWGR
                     AGFDGLSAEDFAALLEYTPEVVVFGAGARLRFPHPRLTAALTNAGIGVEVMDTGAACR
                     TYNVLLSEDRRVLAAVLL"
     gene            7137..8366
                     /locus_tag="PKN55_05715"
     CDS             7137..8366
                     /locus_tag="PKN55_05715"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017508168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HNX70770.1"
                     /translation="MQPIRKSKKLDNVCYDIRGPVLARAKQMEEDGQKIIKLNIGNLA
                     AFGFDAPEEIQQDMIRNLPNSAGYSDSKGIFAARKAIMHYTQEKKIKGVTLEDIYVGN
                     GVSELIVMAMNALLDAGDEVLVPAPDYPLWTAAVSLSGGTPIHYLCDEANDWLPDLDD
                     MRAKITPNTKAIVIINPNNPTGALYPDATLQAIVAMAREHGLIVCADEVYDKVLYDDA
                     VHTSIASLSEDVLTLTFNGLSKNYRSCGYRAGWLVVSGDKRRATDYIEGLNMLASMRL
                     CANVPGQWAIQTALGGYQSIDDLVAEGGRMRRQRDLAHELISAIPGVSCVKPKATLYM
                     FPRLDPAVYPITDDQEFISELLQEEKVLLVQGTGFNWPHPDHFRLVFLPHEDDLREAI
                     GRVARFLEGYRRRHGTA"
     gene            8446..9756
                     /locus_tag="PKN55_05720"
     CDS             8446..9756
                     /locus_tag="PKN55_05720"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008954866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HNX70771.1"
                     /translation="MKPINVGLLGVGTVGGGTATVLKRNAAEISRRAGRDIVLKMAAN
                     LDLDAARAIVGDGVDVVADGFAVARHPDIDIVVELIGGTGIAKELVLAAIDSGKHVVT
                     ANKKLLAEHGNEIFARAQEKGVTVAFEAAVAGGIPIIKALREGLSANHIEWIAGIING
                     TSNFILTEMRDKGSAFADVLAEAQRLGYAEADPTFDIEGHDAAHKLTIMAALAYGIPM
                     QFDKAYLEGISKLTARDIRYAEELGYRIKLLGITRRTDKGVELRVHPTLIPEKRLIAN
                     VDGVMNAVMVKGDAVGATMYYGAGAGSLPTASAVVADIVDVTRLATADPEHRVPHLAF
                     QPDRLADLAILPISEVESSYYLRVNVIDRAGVLAELTRILASEGISIEALIQKGSERE
                     AEAEIVLLTHRVVEKHVNAAIAQIEALDSVTGSVTKLRMEALNG"
     gene            9834..>10273
                     /locus_tag="PKN55_05725"
     CDS             9834..>10273
                     /locus_tag="PKN55_05725"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003746333.1"
                     /note="catalyzes the formation of L-threonine from
                     O-phospho-L-homoserine; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HNX70772.1"
                     /translation="MKYISTRGGMAPATFSDILLAGLAPDGGLAMCESYPQLSRAELD
                     AMRAMSYPELAFDLIRRFADDIPAADLKAIVDKTYTRATYGTDEITPLKRLEDGLYIQ
                     ELSNGPTLAFKDMAMQLLGNLFEYTLAKQGETLNILGATSGDTGS"
BASE COUNT         1923 a         3230 c         3363 g         1757 t
ORIGIN      
        1 gcaccagctc gcgcaaatcc tcggggatgt tggccggcac cgtcaccaat tgctttttaa
       61 cgtttcttga acccacatgg ggccaggatt ctaccgtgcg gcacggtata gccactcacg
      121 catcccgccc cgcaaacaag ccaatcacgc tgctcaaatc tgcctggcca tgtccgcgtg
      181 tggcgtgcag gcgcatcagt tggtccgcca gcgcggccag cgggctggcg ctgtcggttt
      241 cccgcgccag tttcaccgca ttgtccaggt ctttcagcag ggtggccact ttccattgca
      301 ccggctcgtg ctggcgggcg gccatgcgtg gggcgaggat ttgcatgggc agcgaatcgg
      361 caaagccgcc ggccagggcc ggggcgagca ggctggcgtc cacgccgccg cgctcggcca
      421 gcgccacggc ttcggcgatc agcaggctgt tggcggcgac aatcagctga ttgcacacct
      481 tggtgacctg gccgcagccg accgggccca tatgggtcag ccgctggctg agcgccgcca
      541 gcggcgcgcg caggcgctcg atgtcggcgg gctcgccgcc ggccatgatc accagccggc
      601 cttgctcggc gcccaccacg ccgccggaaa ccggcgcgtc cacccagcgc gcgccgcatt
      661 gctcggccag gcgttgggca aaggctcggg tggcggcggg ttcgatgctg gaaaaatcca
      721 ccaccagcgt gttggcgctc agctgggcgg ccaggccgtc ggcggcgaac atcacctgtt
      781 cgacggcggc ggtgtcagac aggcatagac acacaatgtc agcgtcggcg gccagctcgg
      841 ccagggtggc ggcgtggcgg gcgccggcgg cttgcagcgg ggcggttttg gcggggttgc
      901 ggttccagac ggtcagcggg tggccggcgg ccagcaggcg gcggcacatt ggcaggccca
      961 tcaggccaat gccggcaaag gcgatgcgca gggggtggct catggggttt ggctcacaat
     1021 ggcgcggcgc gcccgtcacc gggcgcgccg caatcagatc atccaaatcc agacaatcag
     1081 gtccagacaa tccagggcaa tatcgggtca atcagcaggg gctgggctca acaaaaaagt
     1141 tgcgcagcag tttgcggatc agctcgactt caaagggttt gtcgcagatg gccgacacgc
     1201 cggcctgttc gatggcggcc agccgacctt catcgtgctc ggaggacacc atcagaatgg
     1261 ggactgaagg ttgcatgctg tgctggcgga cgtattccac cagttgcttg ccgtccattt
     1321 ccggcatgtt gtagtcggtc accaccaggt cgaacagcgt ggtgtccagc agcggcacgg
     1381 cttcctggcc gttgatggct tcgctgatgc gctcaaagcc gatgcgttgc agcacgctgc
     1441 ggatatgggt gcgcgcggtc aggctgtcgt ccaccagcag cacgctgagc tcggccacgt
     1501 ccagcacgct ggagtccatg cggttttcgt gggcgttgag aaactcgacg gtgttgttca
     1561 gcgcgtgcga cagctgctcg ctattaaacg gcttgggcag gatggccagg gtgccggcct
     1621 gacggatggg gtcgaggtag gagcgcttgg tttcgctgga caccaataaa aacggggttt
     1681 cgcgcatgcg ggcgtcgtcg cgcatgcgat agaccagatc ggtgccggtc atgtccggca
     1741 ggtagagcgc gctgatcacc acatccggcg gcatcgggct ggacagcaag tccagcgtgg
     1801 cctggccgct ttcagtgacc tggacattgt cgatgcccag tttgttcagc atggagacaa
     1861 tgatgttggc ctgcaacgag gacggctcgg ccagacagat attcaggtat tcgcgttcca
     1921 gcatggcagt ttccgggacg agggtaagtg ggcaacagaa taacccaaga cgttagcatg
     1981 gatatgccga tttgtgcagt gtctggctga cggaagttgc gcaggctggc cataaaaaaa
     2041 ccggcgcgca cccgaaggcg cgcgccggct tggcgctcaa ggcttaacga cgatcagtcg
     2101 cgaaagctcg ggtagcggtc gcggcggccg gcgccagcgg caaagccgcc ttcgcgcgga
     2161 cgacgctcct ggctgccgcc gtggccgtgg ctcttgccgc catgaccctg gccctgaccc
     2221 gccgggcctt gacggcggtc gccgccggca aagctgcggc catcacgacg gccggcgttc
     2281 gggaacgggt tgtagttgga gttgtcggac ggctgacgct cgtcgtacgg cgaacgggcg
     2341 cgcggctcgc gggcttgctg gccttcgcct tgcggacgcg gcgcgcgatc ctgacggccg
     2401 gcaaacggct ggcggtcgcg ctgcacgaac ggacggccgt cgcggttgtc gcggccttcg
     2461 tgacgcggct ggcggtcgcc aaagtggcca cggttggcga cgtgctcgcc ggcggcgcct
     2521 tcgctgcggg cgttggcgcc catgccgccg cggtcgccaa actgacggtc gccgccacgg
     2581 tcattgaacg aacggtcgcc gcgatgctcg cgcggttgat ggtcgcggtc gccgaacgac
     2641 ttcttgccgc cccagccgcc gccgccattc gggcgcttgc cgccatgcgg acgaccaccg
     2701 ccggcacccg gcttgcggtc ttgcggacgg aagcggcttt ccatgccttc cacttcgtgc
     2761 acgcgaatct tgtggctggt gaagcgctcg atgcggcgca gcggcatcac gtcgtcacgg
     2821 cccaccaggg caatggcggt gccggtggcg ccagcgcggc cggtgcggcc aatgcggtgc
     2881 acgtagtctt cggcaaagcg cggcaggtcg aagttgaaca catggctgat gccggccacg
     2941 tcgatgccgc gggcggccac gtcggtggcg accaacagat cgaactcgcc acggcgcagc
     3001 tgggtcagcg tgcgggtgcg ctcgcgctgc ggcatgtcgc catgcagggc ggcggcgcgc
     3061 aggccttcca cgcacagttc gtcggccagc tcttcggtgt cacgcttggt ggcggtgaag
     3121 ataatggctt gcttgatgcc ttcttgcgcc aggaagtgct tgagcaggcg ttttttgtgg
     3181 cccagatcgt ccacatagca cagcatctgg tcgatctgtt cgtggcgggc ggtttgcggc
     3241 gccacttcca cggtggccgg atcgttcagc agctggctgg cgaaggtgcg cacattgtgg
     3301 gtcatggtgg cggtgaagaa cgcggtctgg cgttctttcg gcaactggtt ggcgatggcc
     3361 agcacgtcgt cagagaaacc catgtccagc atgcggtcgg cttcgtccag caccagcatt
     3421 tccaggcgcg agaagtcgat gcggcgggca ttgacgtggt cgatcagacg gcccggggtg
     3481 gccaccagga tttcgtgcgg cttggccagc aggtcgttct ggatgcggta ggacacgccg
     3541 cccaccacac acacggtttt catgcgttgc aggcccttgg agaaggtttc tgccgcgcgc
     3601 gccacttgct gcgccagttc gcgggtgggg gtcagcacca gcacgcgggc gccacggccc
     3661 ttttcacccg gggtttgcgc caggcgatgc agcgccggca gcaggaaggc agcggtcttg
     3721 ccggtgccgg tttgcgctga gccaatcagg tcgcgacctt gcagcagcag cggaatggtt
     3781 tgctcctgca ccggggtcgg cgtggtcagt tggttttggg tcagcgtaga aacaatgcga
     3841 gcgtcgaggc cgagcgcctc gaaagcggat tgcgacaagg gaatatcctt tgtgtgtgaa
     3901 gcaagtcaat agagctcggc ggcaagggca ggcgtcaggg cacggggcgg caagacggcg
     3961 gcggcatgtc atgaatcatc gaatgccaac cgtaagaagg tcgatcacac agacggatcg
     4021 acaagagggt cggtagagac gatgcgcctt ccgaaacagg gttcgaaggc aaggccgagc
     4081 ggcaatcacg cagtatcggt gaaaactgcc ggtaatgcaa gtgaaatctg gttcaattac
     4141 acccggtcac gcgctatttt tcattcatgg ccgggtttgc gctggcgcag ctgcttgact
     4201 gcgccgcgtt tggccttgcc gtccagccgg cgggtttgcg aggcgcgggt ggggcgggtg
     4261 gccttgcgtg ggcgcggcgc ctggcaggcg gcctgaatca gcgccaccag cttggccagc
     4321 gcgtcggcgc ggttcagcgc ctgactgcgc tgtgcctgcg actggatcag caacacacca
     4381 tcctgcgtca tgcgccggcc agccagcgcg cacaagcgcg ccttgaccgg ctcgggcaga
     4441 ctgggcgagc gccgcacgtc aaagcgcagc tgcactgcgc tgtcggtggt gttgacatgc
     4501 tgaccgcccg gcccctgggc gcgggtaaag gcggcttcga tttcgtcgtc ggccagcgcc
     4561 aggctgcggg tgatggggat catgattcgg caaacaggca gcaaaacatc aagtatagct
     4621 cagccgatgg cggggttatg tcaactgatg cgcttgccgc atgcggcagt gcgggcagcc
     4681 tcagggtaag atacgtcatt cgttttaatg gagcgccgta tgttttcctg gttcctgcgc
     4741 atgagcatcc gctggaagct gcagctgggt ttttttgtgg tgaccatgat caccaccatc
     4801 ttcaatcgcc tgctggccac ccacgaactg agcaagatga tcgaaatcgc ccgcgccgac
     4861 caggtgccgg cggcggtgct ggcacagatg atggataacc gcagcaccta tatctttaat
     4921 tcgttctggg aatccggact ggagttcatg gtgcagttca tggtgatcgg ctttgtcgcc
     4981 cgccagttgg tgcgcccgat tcaagagctg cgcgatgcca tgcaggccat gtcgcgcggc
     5041 gatctggtgc accggctgca agaaaccgcc cgtgacgagt tgggtgaatt gcaggccagc
     5101 tttaatctga tgcgccgccg ctttgccgac atcctgcgcg agatcgaaaa cagcggcaag
     5161 cagatgcatc agtcggcgtt tcaggtgacc accattgccc gcgaaatcgc cgaagtcagc
     5221 cgcaaggaag aaagccgctc ggtggaagtt caccaggtga cgcgcagcct gagcgatatt
     5281 gcccatcagg tggaacagcg cgcgcaggcg gccatcgagc aatccaccct gctggaaaac
     5341 cgtggactgg aaggcatcga cagtgtgcgg cgcaatatcc agatgatgga tgaaactgcc
     5401 actggcgtgg cggcagcctc caccagcatt ggcgagctgg aagctgaatc ggcacgaatt
     5461 cacgccatca tcgacaccat tcacgacatc gccggccaga ccaatctcct ggcgctgaat
     5521 gccgccatcg aagcggcccg cgccggcgag ctgggccggg gttttgccgt ggtggccgac
     5581 gaagtgcgca agctggccga gcgctccagc gcctcggcgc aggaagtggc caacatcatc
     5641 cagggcctgg gggtgcgcgt gcgcgaagtc accggctcaa tgcagaacgt ggtggagcag
     5701 gtggcggatg gacgtcaggt ggccaaccgg acggtggaag tgatcgaagg catggtgcag
     5761 gaaatcagcg tggcggccga aggcagccgc gccatcggcg agggcagcca gacccaggtg
     5821 gccgaactgg gccgcttgca acacacgctg gaagcactgt tcgccaccct gcacgaaagc
     5881 ggctcgaaag tcaccgccac cgccgccatc ggcgaaacga tttttgaggt gagcgagcgg
     5941 ctcaaccaga ccatgggcgg ctttaacttc cgccgggaac tgcaaaccag ccgcaccaca
     6001 gaagaaaaac gccgcttccc gcgtgcggaa aacagcctgc gcgtgcatgt ggtgcacgaa
     6061 gagcgcgcct ttgagggcat ctcgctggac atcagtttgt ccggcctgcg cctgagcctc
     6121 gggcaggatc agattttacc ctcgcaagcg ctgctgggcc tgaagctgta catgccgcgc
     6181 agcagcctgg aagaattccg caaccagcag cccatcagcc tgagcggccg ggtgatgtgg
     6241 ttgcgcaagg atggcgagca cacactgtat ggcattcagt ttgaaaatct gaatgaagcc
     6301 agccgccagg cgctgcgcca ggcggtgacg tatttcaaca aagcaccgga ataccaataa
     6361 cacgcctgat ccaccgtgcg ccctcaagcg cacggctcac catgtgagcg tcgcgcacca
     6421 aactcagcac agcggacctg gccaggcacg cgcgggttac aacgccaggg agtgtgtgcg
     6481 cggcgcttac agcaggacgg cggccagcac gcggcggtct tcgctgagca gcacgttata
     6541 agtgcggcag gcggcgccgg tgtccatgac ttccacgccg atgccggcat tggtcagcgc
     6601 ggcggtcagc cggggatgcg gaaaacgcag ccgcgcgcca gcgccaaaca ccaccacttc
     6661 cggggtgtat tccagcaggg cggcaaaatc ctcggcgctc aggccgtcaa agccggcgcg
     6721 gccccagggg gccacgtgct cgggggtgat caacacattt tcggtgtagc gggtctggtt
     6781 cacgtccaga tagccgtcgc catactgggt gaccaggttc agcgggctgg tgtgcaggtg
     6841 cagtttcatg cgttcagagt ccttgagtgg ccgatgtgcg cactgtcccg gcgcgcgtct
     6901 ggcgcaatgt gacagaatgg tgcgcggatt cgtgcgttgc acaacgcttt tgcggtagga
     6961 ttataagttc cactttgact cgcgcgtaag ccgccgctcg cagaatgcgc cggccgcgcg
     7021 caccactcag gagcgtgatc gtgactgccc gccgcgccaa tgcagccaaa gccccgcgcg
     7081 gccagcgtag cccgcgggcg cgtgtgttgc cagaacccaa gccggatttc acccctatgc
     7141 aacctattcg caaatcgaaa aagctcgaca acgtctgcta cgacattcgt ggcccggtgc
     7201 tggctcgcgc caagcaaatg gaagaagacg gccagaaaat catcaagctg aatatcggca
     7261 atctggcggc gttcggtttt gacgccccgg aagaaatcca gcaggacatg atccgcaacc
     7321 tgcccaattc ggccgggtat tctgattcca agggcatttt cgccgcccgt aaggcgatca
     7381 tgcattacac ccaggaaaaa aagatcaagg gtgtgacgct ggaagacatt tacgtcggca
     7441 atggcgtgtc cgagctgatt gtgatggcga tgaacgccct gttggacgcc ggcgacgaag
     7501 tgctggtgcc ggcgccggat tatccgctgt ggaccgcagc cgtcagcctg tccggcggca
     7561 cgccgattca ttacctgtgt gacgaggcca acgactggct gcctgatctg gacgatatgc
     7621 gcgccaagat cacccccaac accaaggcca tcgtcatcat caacccgaac aacccgaccg
     7681 gcgcgctgta tccggacgcc acgctgcagg cgattgtcgc catggcgcgc gaacacgggc
     7741 tgattgtctg tgccgatgaa gtgtacgaca aggtgctgta cgacgacgcc gtgcacacct
     7801 cgattgcctc gctgtcggaa gacgtgctga cgctgacctt caacggcctg tcgaaaaact
     7861 accgctcctg cggctaccgc gctggctggc tggtggtgtc tggcgacaag cgccgcgcca
     7921 ccgattacat cgaaggcctg aacatgctgg cctcgatgcg cctgtgcgcc aatgtgcccg
     7981 gccaatgggc catccagacc gcgctgggcg gctatcaaag catcgacgac ctggtggccg
     8041 aaggcgggcg catgcgtcgc cagcgcgacc tggcgcatga gctgatcagc gccattccgg
     8101 gggtcagctg cgtcaagccc aaggccacgc tgtatatgtt cccgcgcctg gatccggcgg
     8161 tgtacccgat taccgacgat caagaattca tcagcgaact gttgcaggaa gaaaaagtcc
     8221 tgctggtgca gggcacgggc tttaactggc cgcacccgga ccatttccgc ctggtgttcc
     8281 tgccgcacga agatgacctg cgcgaggcca ttggccgcgt ggcgcgtttt ctggagggtt
     8341 atcgccgccg ccatggtacg gcataatcca cgccaagcct gaatcggccg gctgaccccg
     8401 gcctgaccgt cactcaacca aaaaaactgt gggaaacaag caagcatgaa accgatcaat
     8461 gtaggcctgc tgggcgtggg gactgtgggt ggcggcaccg ccaccgtatt aaagcgcaac
     8521 gcagcggaaa tcagccgtcg cgccggccgt gacattgtcc tgaaaatggc cgccaacctg
     8581 gatctggacg ccgcccgcgc catcgtcggc gacggcgtgg acgtggtggc cgacggcttt
     8641 gccgtggccc gccacccgga catcgacatt gtggtcgaac tgatcggcgg caccggcatc
     8701 gccaaagaac tggtgctggc cgccatcgac agcggcaagc atgtggtcac cgccaacaaa
     8761 aaactgctgg ccgaacacgg caacgaaatc tttgcccgcg cccaggaaaa aggcgtgacc
     8821 gtggcgtttg aagccgccgt ggccggcggc atccccatca tcaaggcgct gcgcgaaggc
     8881 ctgtcggcca accacatcga atggattgcc ggcatcatca acggcacctc caacttcatc
     8941 ctgaccgaaa tgcgcgacaa gggctccgcc tttgccgacg tgctggccga agcccagcgc
     9001 ctgggctacg ccgaagccga cccgaccttt gacatcgaag gccacgacgc cgcgcacaag
     9061 ctgaccatca tggccgcgct ggcctatggc atcccgatgc agttcgacaa ggcctatctg
     9121 gaaggcatca gcaagctgac cgcccgcgac atccgctacg ccgaagagct gggctatcgc
     9181 atcaagctct tgggcatcac ccgccgcacc gacaagggcg tcgagctgcg cgtgcacccg
     9241 acgctgatcc cggaaaagcg cctgatcgcc aatgtggacg gcgtgatgaa cgcggtgatg
     9301 gtcaagggcg acgccgtggg cgccaccatg tactacggcg ccggcgccgg cagcctgccc
     9361 accgcttccg ccgtggtggc cgatattgtt gacgtcaccc gcctggccac cgccgacccg
     9421 gaacaccgcg tgccgcacct ggccttccag ccggaccgcc tggccgacct ggccattctg
     9481 ccgatcagcg aagtggaaag ctcctactac ctgcgcgtga atgtcatcga ccgcgctggc
     9541 gtgctggccg agctcacccg catcctggcc agcgaaggca tctcgatcga agcgctgatc
     9601 cagaagggca gcgagcgcga agccgaagcc gaaatcgtgc tgctgaccca ccgcgtggtg
     9661 gaaaagcacg tcaacgccgc catcgcccag atcgaggcgc tggacagcgt caccggcagc
     9721 gtcaccaagc tgcgcatgga agccctcaac ggctgatccc cgtcccgtcg ccccgcgccc
     9781 cggcgcgggg cgtttgccgt tctgacacgc gcaattgcgc caagcgaccg aacatgaaat
     9841 acatcagcac ccgcggcggc atggcccccg ccaccttctc tgacatcctg ctggccggcc
     9901 tggcccccga cggcggcctg gccatgtgcg aaagctaccc gcagctgagc cgcgccgagc
     9961 tcgacgccat gcgcgccatg agctacccgg aactggcctt tgacctgatc cgccgctttg
    10021 ccgacgacat cccggccgcc gacctgaaag ccatcgtcga taaaacctac acccgcgcca
    10081 cctacggcac ggatgaaatc accccgctca agcgcctgga agacggcctg tatatccaag
    10141 agctgtccaa cggcccgacc ctggccttca aagacatggc catgcagctg ctgggcaatc
    10201 tgtttgaata caccctggcc aagcagggcg aaaccctgaa catcctcggc gccacctccg
    10261 gcgacaccgg ctc
//
LOCUS       DAOPYX010000080        10272 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_7590_length_10272_cov_7.839777, whole genome shotgun sequence.
ACCESSION   DAOPYX010000080 DAOPYX010000000
VERSION     DAOPYX010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 10272)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10272)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10272
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7590_length_10272_cov_7.839777"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(180..449)
                     /locus_tag="PKN55_05730"
     CDS             complement(180..449)
                     /locus_tag="PKN55_05730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX70773.1"
                     /translation="MIKIQDAEWTTVGQMWWAFVWRVVVIGTLIGLVVGFIFGFLGAI
                     MGNTAMGQMMGMGFSMLLNLPVSIWAYRAALTKRFDGYQLALVEG"
     gene            complement(500..1165)
                     /locus_tag="PKN55_05735"
     CDS             complement(500..1165)
                     /locus_tag="PKN55_05735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX70774.1"
                     /translation="MKSSLPFLPALLACLTMPAIAASFDCGQARTPVERAICTDPSLS
                     AQDEQLALLFEQAKLHGERSEVIRQQKGWLKTRDACKDLTCLRKQYKSQVQLLQPVAA
                     AIGQGMPWTGRYIVNRDNELAIRQIGPKQIEFDFMGCGYSASGTISCNGVHGVARVQG
                     SRGHFSAEDCNLQFYRHEDGSMSVDQQGICDLGAYVTMGNQYRRVSKAVPTFPYKSVL
                     DGM"
     gene            complement(1162..1635)
                     /locus_tag="PKN55_05740"
     CDS             complement(1162..1635)
                     /locus_tag="PKN55_05740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX70775.1"
                     /translation="MTHLPLKPLLLAAGLFMASAQAATPDIQPYLGKWRTDDGEVLAL
                     NAKGDIRIQQANRKGDCTGHYANRYQTLSREQLVKDLQRFIRELDPEEDDIHTQRRLA
                     NEQLQQLRKNAQYTSLFSMCSDGSRTHVFISPSHGFYLEEGGGNEWLVLMRKVQP"
     gene            complement(1753..2043)
                     /locus_tag="PKN55_05745"
     CDS             complement(1753..2043)
                     /locus_tag="PKN55_05745"
                     /inference="COORDINATES: protein motif:HMM:NF022592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2917 domain-containing protein"
                     /protein_id="HNX70776.1"
                     /translation="MTPTRHCVAAGQLLTLPARAGQLARCASGVLWLTAGDGDVVLRP
                     GDSYPVPADGPLLVEAADQGVLLLETPNPQARRRPRPLRYPGLGRGAVWVLG"
     gene            2401..3822
                     /locus_tag="PKN55_05750"
     CDS             2401..3822
                     /locus_tag="PKN55_05750"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HNX70777.1"
                     /translation="MNATTPTLLYQHLADDVASMIASGTLRPGDKLPSLREMRTRRQV
                     SLTTVTEAFRVLEDRGLIEARPQSGFYVRRRPPLPLPAQSSPSPEPSLVSVNALLWRY
                     MRIAHAGTFGCAVPAAELFPGAQLQKLTSEALRRHGHLLSDYGKPAGLDSLRRQIARR
                     ALGWGGRLAVDDIIVTNGCIEALSLCLRAVAQAGDVVAIESPTYFGVLQLLESHGVKA
                     LEIPTHPVSGVSLEALELATRHGQVKACLLTPNFSNPLGSLMPEANKRALVEMLAERN
                     IPLIEDDIYGEFHFGRERPLPAKAFDTCGNVLYCSSFTKMISPGLRVGWVCGGRYQAK
                     LEVLKYINSFTTPALMQQVVAQFLESGFDRHIRRLRRACADQVAQAMDGIERYFPAGT
                     RLNAPQGGYVLWVELPPQADTVALMETAAQENISLAPGALFSAREGFASSMRLSCGAP
                     WTPAQDQALRRLGELVDAHLQMA"
     gene            complement(3996..5153)
                     /locus_tag="PKN55_05755"
     CDS             complement(3996..5153)
                     /locus_tag="PKN55_05755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiH/UbiF family hydroxylase"
                     /protein_id="HNX70778.1"
                     /translation="MNTCFDALIVGGGLVGASLALALARQGRQVALIEGQQPPLTELD
                     RPDDWDPRVYAISPANQAFLQGLHAWPDASRLGVVAGMDVRGDRHGRIAFHAADAGQP
                     ALAWIAENRWLLAALWQQLADSPAQCIAGVRPTAISQHPRHVSLTLDDGRSLNTRLLI
                     GADGANSWVRQQTGIRVDVSPYGHSGVVANFVAERDHGNIARQWFLGDGILAWLPLPG
                     QRISMVWSTAKPEALTALDAATLCDTVAQAGGRELGGFSLLTPAHAFPLRLMRPDSVV
                     APRIALVGDAAHTVHPLAGQGVNLGFGDAHALATLLAEHGGDPGEWLLLRRYQRMRRE
                     AVNTMQLTCDGLFKLFHTQDPLLGWLRNTGLSLTQHLPLIKRQLARQAIGF"
     gene            complement(5186..7036)
                     /locus_tag="PKN55_05760"
     CDS             complement(5186..7036)
                     /locus_tag="PKN55_05760"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HNX70779.1"
                     /translation="MKKLLYLACLASLPAWAALDADLVAARNGGPALFAAQDSAQGTV
                     LESYPRYWALSRNLDAAGSGEVQAFLQRYADSALAEKLRLDWAKRLARSEAWAEFAQE
                     YAKLPAVARDDDVQCSDTLLRLKQGDGEALAQARQRWLTQRTATPGCAALFDQLSGRL
                     SEADIWRRVRLMLAAGNVNSARAISQATGQPLSAAQVTSPGVGDLSTQAGRESALFAI
                     VRQAKNDPQGAGQLLASLEGRLPKTDAGYGWGQVGMAAAKRQDGWGALSAYAKADKRQ
                     LSDEQWEWWARAALRVGHWETVAQVTEDMPEALRNQPAWTYWQGRALKQRGKTQDANQ
                     RFTRISQLNSFYGLLARDELGTSVGETPARYTPSEQEIASMRQNPSLARALALFDIAQ
                     RFARPEFREDAKREWRFAMRNQPDESLLAAAEIGRQAGFYDMAIYSADRTVNLHDFTL
                     RYLSPYRDATQRYARQLNLDEAWVYGLIRQESRFVHIARSGVGASGLMQLMPATAKWA
                     ANKMGLGSFSVNDVSTNIQLGTWYLRHVFDGLDDNAVLATAAYNAGPGRARAWQSGDT
                     LEGAIYAETIPFTETRDYVQKVMANAVHYSGHLGSARLRLKDRMGWIPGR"
     gene            7331..8434
                     /gene="ribBA"
                     /locus_tag="PKN55_05765"
     CDS             7331..8434
                     /gene="ribBA"
                     /locus_tag="PKN55_05765"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012697858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HNX70780.1"
                     /translation="MLSPIQDIIADIKAGKMVILVDEEDRENEGDLVLAAEHVTPEAI
                     NFMAKYARGLICLTLSEARCRQLNLPLMVSNNGSSHGTNFTLSIEAAEGVTTGISAAD
                     RARTVQAAVARHAKPSDIVQPGHIFPLKAQNGGVLVRAGHTEAGCDLSALAGLEPASV
                     ICEIMNDDGTMARLPELLVFAEQHGLKVGAISDLIHYRSRHECLIEQVGKRQVATPFG
                     EFTLHAFRDNTTNETHLALSKGDIRPDADTLVRVHEPLSVMDWLDAGQSPHSWSVAQA
                     LAKINADGKGVVVLLHRTEHGAELLERALPDLNAERPRAKWDAKTFGIGAQMLKALGV
                     GRMTLLSQPRKIPSMTGFGLEVSGYLLPEAAHA"
     gene            8487..8960
                     /gene="ribH"
                     /locus_tag="PKN55_05770"
     CDS             8487..8960
                     /gene="ribH"
                     /locus_tag="PKN55_05770"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012697857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HNX70781.1"
                     /translation="MHPDIQIVEPNLDGAELRIGVVMSRFNPVVCHGLRDACLDELIK
                     LGVAAEDITLVTVPGALEIPLALQTLAMSEDEDFDALIALGAVIRGETYHFELVSNES
                     GAGVTRVGLDTGVPIANAILTTETDEQAEARMEVKGREAARVAVEMVNLNKSFFE"
     gene            8957..9412
                     /gene="nusB"
                     /locus_tag="PKN55_05775"
     CDS             8957..9412
                     /gene="nusB"
                     /locus_tag="PKN55_05775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012697856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HNX70782.1"
                     /translation="MKTARRRAREFAVQGVYQWQLNAHTASTIEKNLRENEFFAKADE
                     ALFRTLLFGVLQDIASLDEQLQRHYERAPEEVSPVERAILRVAALELTQHPETPYPVI
                     INEAIEIAKTFGGADGHKFVNGVLDKLAADVRADEVEAQKRQRRNSRAG"
     gene            9602..10270
                     /locus_tag="PKN55_05780"
     CDS             9602..10270
                     /locus_tag="PKN55_05780"
                     /inference="COORDINATES: protein motif:HMM:NF015438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HNX70783.1"
                     /translation="MLPSLTLSGLFIGQLAHLPGDGTPTGMYKQPVDEAWATVDGLRG
                     DVQADRRFHGGPDKAIHHYPAEHYARLAAHAPQAAAALRAGVLGENLSTTGWDETCVC
                     LGDCYQVGEALLQVTQPRKPCWKINARLEVEQLSMFIAEHSLTGWYCAVRQPGRLWPG
                     APVTLVSRPADTLSLAQFNALQAEHRPDPARLRTVAAIDGLAADVAQRLRERAAWLDG
                     LRAG"
BASE COUNT         1901 a         3268 c         3239 g         1864 t
ORIGIN      
        1 aacaaggcat gagaaagcaa aagggaacca ggcatggtca gagtgtccgt ggtaagtcaa
       61 ttagcccatc ggggctgatg gcatcggcta tccgcccaac agccttcggg gcgacaacaa
      121 gtctggctat tgtcgcaccg aaggcaatgc aagtgctagc gcatgctgtg tgcagcagtt
      181 cagccttcca ccagcgccag ctgataccca tcaaaccgct tggtcaacgc ggcgcggtag
      241 gcccagatgc tcactggcag gttgagcagc atggaaaacc ccatgcccat catttgcccc
      301 atcgctgtat tgcccataat ggcgccaaga aaaccaaaga tgaagccaac caccaggcca
      361 atcaaagtgc caatcaccac cacccgccag acaaacgccc accacatttg cccgaccgtg
      421 gtccattctg catcctgaat cttgatcata tttttctcct gtttcgttgc ttgttcgtga
      481 cgtgcattga gatgacgtgt cacattccat ctagtacgct tttataagga aatgtcggca
      541 cagctttgga gacccgacga tactgatttc ccattgtcac gtaagcgcct aaatcgcaaa
      601 tgccctgctg atccaccgac atgctgccat cttcatggcg ataaaattga aggttgcaat
      661 cttctgccga aaaatgaccg cgcgagcctt gtacacgggc tacgccatgc acgccattgc
      721 aggaaatggt tccgctagca gaatagccac accccataaa atcaaattca atctgtttgg
      781 ggccaatttg gcgaatggcc agctcattat cacgattgac gatatatcgc ccggtccatg
      841 gcatgccctg gccaatagca gcggccactg gctgcaaaag ctgtacttgt gatttgtatt
      901 gcttgcgcag gcaggtcagg tctttgcagg catcgcgggt ttttaaccag cctttttgct
      961 ggcggatcac ttcgctacgt tcgccatgca gcttggcttg ttcaaatagc aaggccagtt
     1021 gctcgtcctg cgccgacagt gacgggtcgg tgcaaattgc tctctccact ggcgttctgg
     1081 cttggccgca gtcgaagctg gccgcaatgg caggcatggt caggcaagcc agcagggcag
     1141 gcaaaaatgg caaggatgat ttcatggttg caccttgcgc atcagcacca gccattcgtt
     1201 gccgccacct tcttccagat aaaacccgtg gctggggctg atgaatacgt gggtgcggga
     1261 gccatcgctg cacattgaaa acaaactggt gtactgggca tttttgcgta gttgttgcag
     1321 ttgctcattg gccagtcggc gctgggtgtg aatatcgtcc tcttccggat caagttcacg
     1381 gatgaagcgt tgcaaatctt tcaccagttg ctcacgagat agggtttggt agcgattggc
     1441 atagtggcct gtgcagtcac ccttgcggtt ggcttgttga atacggatgt cgcctttggc
     1501 gttcaatgcc agcacttcgc catcatccgt gcgccatttg cccagataag gctgaatatc
     1561 cggtgtggct gcctgcgcac tggccataaa caggccagca gcaagcagca ggggtttcag
     1621 gggaagatgt gtcatcagat gctcctcggt ctgtcatcaa agaccatgtg gtgaatgtct
     1681 ggagcatgat gttaactgga taccgcgaca gagcgcgtcg cggtcaagag aaatccacgg
     1741 cttgacattc tactagccaa gcacccacac cgccccccgt cccaagccgg gatagcgtag
     1801 cgggcggggt cgacggcggg cttggggatt cggggtttcc agcaacagca cgccttggtc
     1861 ggcggcttcg accaacagtg ggccgtcggc gggcaccggg tagctgtcgc cggggcgcag
     1921 caccacatcg ccatcgccag cggtgagcca tagcacgccg ctggcgcagc gcgccaattg
     1981 gccggcgcgg gcgggcagtg tcagtaattg gccagcggca acacagtgac gggtgggggt
     2041 catgattggg ctcctgaaag cggtgtgggg acgccaatca tgctaggctg tgtcgcgctg
     2101 cgccggcaga ggcacagtgg cccgattttg accggttttg cctgggcagt tttcaactgt
     2161 cctgcccatg ttgcactgca actgtctctg gcgggccagg gcgacaggcg cgagcataag
     2221 catgacgcgc tgccggcaaa acgccgcaca gccgccacag taaaaaccgc gccgcaagct
     2281 cgcaccttgc cgcttttttg tgttgagcgc agctgagcaa accctcctgg cttgccgtac
     2341 gacttaccct gtctgcgtgc gcctggtttt gtgagcgact ttcaccgcct gattgacacc
     2401 atgaacgcca ccacgcccac cctgctttat cagcatctgg ccgacgatgt cgccagcatg
     2461 attgccagcg gcacgctgcg ccccggcgat aaactgccgt cgctgcgcga aatgcgcacc
     2521 cgccgtcagg tcagcctgac cacggtcacc gaagcgtttc gcgtgctgga agaccgtggg
     2581 ctgatcgagg cgcggccgca gtccggcttt tatgtgcgcc gccgcccgcc tttgccgctg
     2641 ccggcgcaaa gctcgccctc gcccgagccg tcgctggtgt cggtgaatgc gctgctgtgg
     2701 cgttatatgc gcattgccca cgctggcacg tttggctgtg cggtgccggc ggccgagctg
     2761 tttccgggcg cgcagttgca aaagctgacc agcgaggcgc tgcgtcgtca tggtcatttg
     2821 ctcagcgatt atggcaagcc ggccgggctg gacagtctgc gccggcagat tgcccgccgc
     2881 gcgctgggct ggggcggccg gctggcggtg gatgacatca tcgtcaccaa tggctgtatc
     2941 gaggcgctga gcttgtgcct gcgcgcggtg gcgcaggccg gcgatgtggt ggcgattgaa
     3001 tcgcccacct attttggcgt gttgcagctt ctggaaagcc acggcgtaaa ggcgctggaa
     3061 atccccaccc acccggtaag cggcgtgtcg ctggaggcgc tggagctggc cacccgccac
     3121 ggccaggtaa aagcctgtct gctgacgccg aacttctcca acccgctggg cagcctgatg
     3181 ccagaggcca ataagcgcgc cttggtggag atgctggccg agcgcaatat cccgctgatt
     3241 gaagacgata tttatggaga gttccacttt ggccgcgagc ggccgctgcc ggccaaggcg
     3301 ttcgacacct gcggcaatgt gctgtattgc agctcgttca ccaagatgat ctcacccggc
     3361 ctgcgggtgg gctgggtgtg cggcgggcgc tatcaggcca agctggaggt gctgaaatac
     3421 atcaatagtt ttaccacgcc agcgctgatg cagcaggtgg tggcccagtt tctggaatcc
     3481 ggctttgacc gccatatccg ccggctgcgc cgtgcctgcg ccgatcaggt ggcgcaggcg
     3541 atggacggca tcgagcggta ttttccggcc ggcacccggc tgaacgcgcc gcaaggcggc
     3601 tatgtgctgt gggtggagct gccgccgcag gccgacaccg tggcgctgat ggaaaccgcc
     3661 gcccaagaaa acatcagcct ggcgcccggc gcgctgtttt ctgcgcgcga aggctttgcc
     3721 agcagcatgc ggctttcttg cggcgcgccc tggacgccag cgcaagacca ggctttgcgc
     3781 cgcctgggtg aattggtgga tgcgcattta caaatggctt gagcgcatga gcggtgtacc
     3841 ggcctatcgg caagacacgg tagtgaaaga aaatggtaaa tcagtgattg ccggctgtgg
     3901 ccccaccgct gccttgatgc ggctggcttg gcacgactgc agacacggca aggtcgggca
     3961 acaatgccag gagggttgtg tgagcggttc ttggttcaaa acccgatggc ttgcctggcc
     4021 agctgccgtt taatcaaggg cagatgctgg gtgagcgaca ggccggtgtt gcgcagccag
     4081 cccagcagcg ggtcttgggt atgaaacagc ttgaacaggc catcgcaggt cagctgcatg
     4141 gtgttgaccg cctcgcggcg catgcgctgg tagcggcgca gcagcagcca ttcgccgggg
     4201 tcgccgccgt gttcggccag cagggtggcc agggcgtggg cgtcgccaaa gcccaggttg
     4261 acgccctggc cggccagcgg atgcacggta tgggcggcgt cgcctaccag ggcgatgcgc
     4321 ggggccacca cgctgtccgg gcgcatcagt cgcagcggaa aagcatgcgc cggggtcagc
     4381 aggctaaacc cacccagctc gcgcccgcca gcctgggcga cggtgtcgca cagcgtggcg
     4441 gcgtccagcg cggtcagcgc ctcgggcttg gcggtggacc acaccatgga aatccgctgc
     4501 cccggcaagg gcagccaggc caggatgcca tcgcccaaaa accattggcg ggcgatattg
     4561 ccgtggtcgc gctcagcgac aaaattggcc accacgccgc tgtggccata cggcgagaca
     4621 tccacgcgga tgccggtttg ctggcgcacc caggaattgg ccccgtcggc gccaatcagc
     4681 aggcgggtgt tcaggctgcg gccgtcgtcc agcgtcaggc tgacatggcg cggatgctgg
     4741 ctgatggcgg tgggacgcac gccggcaatg cattgtgccg ggctgtcggc cagttgctgc
     4801 cacagcgccg ccagcagcca gcggttttcg gcaatccagg ccagcgccgg ctggccggcg
     4861 tcggcggcgt gaaaggcaat tcggccgtgg cggtcgccgc gcacgtccat gccggccacc
     4921 acgcccaggc gggaagcatc cggccaggcg tgcagtccct gcaaaaaagc ctgattggcc
     4981 ggactgatgg cgtacacccg tggatcccag tcgtcggggc ggtccagctc ggtcaatggc
     5041 ggctgctggc cttcgatcaa ggccacctgg cgtccctggc gcgccagcgc cagcgccagg
     5101 ctggcgccca ccaggccacc ccctacaatc agcgcgtcaa aacatgtgtt catgatgcaa
     5161 ggttctttcc tgttgccatc gtcgctcaac ggccgggaat ccagcccatg cggtctttca
     5221 gccgcaagcg ggcgctgccc agatgacccg aataatgcac ggcgttggcc atcacttttt
     5281 gcacataatc gcgggtttcg gtaaacggga tggtctcggc gtaaatcgcc ccttccagcg
     5341 tatcgccaga ttgccaggcg cgggcgcggc ccggcccggc gttgtaggcg gcggtggcca
     5401 gcacggcgtt gtcgtccagg ccgtcgaaca catggcgcag ataccaggtg cccagctgga
     5461 tattggtgga gacatcgttc accgagaaac tgcccagccc catcttgttt gccgcccatt
     5521 tggcggtggc cggcatcagc tgcatcaggc cagaagcgcc cacgccggag cgggcgatgt
     5581 gcacaaagcg gctttcctgg cgaatcaggc catacaccca ggcttcgtcc agattcagct
     5641 ggcgggcgta gcgctgggtg gcgtcgcggt agggcgacag atagcgcagg gtaaagtcgt
     5701 gcaggttcac cgtgcggtcg gcgctataaa tggccatatc gtaaaagccg gcctgacggc
     5761 cgatttccgc cgccgccagc agcgattcat ccggctgatt gcgcatggca aagcgccatt
     5821 cgcgcttggc gtcttcgcgg aattccgggc gggcaaagcg ctgggcaata tcaaacaagg
     5881 ccagcgcgcg cgccagcgac gggttttgcc gcatgctggc gatttcttgc tcggacgggg
     5941 tgtagcgcgc cggggtttcg cccaccgacg tgcccagctc gtcgcgggcg agcaggccat
     6001 aaaaactgtt cagctggctg atgcgggtaa agcgctggtt ggcgtcctgg gttttgccgc
     6061 gctgtttgag cgcgcggcct tgccagtaag tccaggccgg ctggttgcgc agcgcctcgg
     6121 gcatgtcttc ggtgacttgg gcgacggttt cccagtggcc cacgcgcagc gcggcgcgcg
     6181 cccaccattc ccattgctcg tcgctgagct ggcgcttgtc ggccttggcg taggcgctga
     6241 gcgcgcccca gccgtcctgg cgtttggcgg cggccatgcc cacctgaccc cagccatagc
     6301 cggcgtcggt tttgggcagg cggccctcca ggctggccag cagctggcca gcgccttgcg
     6361 ggtcgttctt ggcctgccgc acgatggcga acagcgcgct ttcgcggccg gcttgggtgg
     6421 acagatcgcc cacgccaggg ctagtcacct gcgcggcgga cagcggctgg ccggtggcct
     6481 ggctgatggc gcgcgcgctg ttgacattgc cagcggccag catcagccgc acgcgccgcc
     6541 agatgtccgc ctcagacaag cgcccgctta actgatcaaa caaggccgcg cagccgggcg
     6601 tggcggtgcg ctgggtaagc cagcgctgac gcgcctgcgc cagcgcctcg ccatcgcctt
     6661 gctttaagcg cagcagggtg tccgagcatt gcacatcgtc gtcgcgcgcc accgccggca
     6721 gcttggcgta ctcctgggcg aattcagccc aggcttcgct gcgcgccagg cgcttggccc
     6781 agtccaggcg cagtttttct gccagggcgc tgtcggcgta gcgttgcaaa aaggcctgca
     6841 cttcgccgct gccagcggcg tccagattgc ggctgagcgc ccagtagcgc gggtaacttt
     6901 ccagcaccgt gccttgcgcg ctgtcttgcg cggcaaacag ggccgggccg ccattgcgcg
     6961 cggccaccag atcggcgtcc agcgccgccc aggcgggcag cgaggccaga caggccaggt
     7021 aaagcagttt tttcatgcca atactcagtc gataagccag acagcacatc tggcaaggaa
     7081 gtgttgcatt ttaagccatt gccctgcccg cgcaggcggg aatgccaaaa acccggcaaa
     7141 aaaattttgc gccggccgcc ctggcccccg gcgatggccc gctgacggcc ggcatattgc
     7201 caaatacact atcgatagcg ccatcgcgcg cgcggaaatc ggcgcactgg ggtatgatag
     7261 aagaactgca cttcacacac cggccgcctg gccggctctg gttgattctt ccgaattcct
     7321 ttagtccatt atgctcagtc ctattcaaga catcatcgcc gacatcaagg ccggcaagat
     7381 ggtcattctc gttgacgagg aagaccgcga aaacgaaggc gacctggtgc tggcggccga
     7441 acacgtcacc ccggaagcca tcaacttcat ggccaaatac gcgcgcgggc tgatttgcct
     7501 gacgctgtcc gaggcgcgct gccgccagct caacctgccg ctgatggtca gcaacaacgg
     7561 ctcgtcgcac ggcaccaact tcaccctgtc gatcgaagcc gccgaaggcg tcaccaccgg
     7621 gatttccgcc gccgaccgcg cccgcaccgt gcaggccgcc gtggcccgcc acgccaagcc
     7681 cagcgacatc gtgcagccgg ggcatatttt tccgctcaag gcgcaaaacg gcggcgtgct
     7741 ggtgcgcgcc ggccacaccg aagccggctg cgacctgtcc gccctggccg gcctggagcc
     7801 cgcctcggtg atctgcgaaa tcatgaacga cgacggcacc atggcccgcc tgcccgagct
     7861 tttggtgttt gccgagcagc acggcctgaa agtcggcgcg atttctgact tgatccacta
     7921 ccgcagccgc cacgaatgcc tgatcgagca agtgggcaag cgccaggtgg ccacgccgtt
     7981 tggcgaattc accctgcacg ccttccgcga caacaccacc aatgaaaccc atctggcgct
     8041 gtccaagggc gacatccgcc cggacgccga caccctggtg cgcgtgcacg agccgttgtc
     8101 ggtgatggac tggctggacg ccgggcaaag cccgcattcg tggagcgtgg cccaagcgct
     8161 ggccaagatc aacgccgacg gcaagggcgt ggtggtgctg ctgcaccgca ccgaacacgg
     8221 cgccgagctg ctggagcgcg cgctgcccga cctcaacgcc gagcgcccgc gcgccaagtg
     8281 ggacgccaaa acctttggca tcggcgcgca aatgctcaag gcgctgggcg tgggccgcat
     8341 gaccctgctc tcgcagccgc gcaagatccc cagcatgacc ggttttggcc tggaagtgtc
     8401 gggctatttg ctgcccgagg cggcccacgc gtaaaatcac ctgttttgcg acggccgcca
     8461 tggccgtcgc ggcaatggag actcccatgc atcccgatat ccaaatcgta gagcccaacc
     8521 tggacggcgc cgaactgcgc attggcgtgg tgatgagccg ctttaacccg gtggtgtgcc
     8581 acggcctgcg cgacgcctgc ctggacgagc tgatcaagct gggcgtggcc gccgaagaca
     8641 tcaccctggt gaccgtgccc ggcgcgctgg aaatcccgct ggcgctgcaa accctggcca
     8701 tgtctgaaga cgaagacttt gacgcgctga tcgccctggg cgcggtgatt cgtggcgaga
     8761 cctaccattt tgagctggtg tccaacgaat ccggcgcggg cgtcacccgc gtgggcctgg
     8821 acaccggcgt gcccatcgcc aacgccatcc tgaccaccga aaccgacgaa caagccgaag
     8881 cccgcatgga agtcaaaggc cgcgaagccg cccgcgtcgc cgtggaaatg gttaatctga
     8941 acaagagctt ttttgaatga aaactgcccg ccgccgcgcc cgtgaatttg ccgtgcaagg
     9001 ggtgtaccaa tggcaattga acgcccacac cgccagcacc atcgaaaaaa acctgcgcga
     9061 aaatgaattt ttcgccaagg ccgacgaagc cctgttccgc accctgctgt ttggcgtgct
     9121 gcaagacatc gccagcctgg acgagcaact gcaacgccac tacgaacgcg ccccggaaga
     9181 agtcagcccg gtggagcgcg ccatcctgcg cgtggccgcg ctggagctca cccaacaccc
     9241 ggaaaccccg tatccggtga tcatcaacga agccatcgaa atcgccaaaa cctttggcgg
     9301 cgccgacggg cacaaatttg tcaacggcgt gctggacaaa ctcgccgccg acgtgcgcgc
     9361 ggatgaagtc gaagcgcaaa aacgccaacg ccgcaacagc cgggcggggt aagccaggcc
     9421 agggcgacac tgaaagaaaa accgcccatc ccgccatgca gacaaaagcg cgccaggaga
     9481 tactcttggc gcgtttttta taggctcgcc cgcaacacta ccgtgcccga tggccaatcc
     9541 ccgcgccacc accctggccc ttcccgtaca attcccttcc tgcccttttc cggtttatcc
     9601 catgctcccc tctctgactc tttccggatt attcatcggc caactggctc acctgcccgg
     9661 cgacggaacc cccaccggca tgtataaaca gccggtggac gaggcctggg ccacggtgga
     9721 tggtttgcgt ggcgatgtgc aggccgaccg gcggtttcat ggcgggccgg acaaggccat
     9781 tcaccactat ccggccgagc attacgcccg gctggccgcc catgcgccgc aagccgccgc
     9841 tgcgctgcgc gccggggtgt tgggcgaaaa cctgtccacc accggctggg acgaaacctg
     9901 cgtgtgtctg ggcgattgtt atcaagtggg cgaggcgctg ttgcaagtca cccagccgcg
     9961 caagccctgt tggaaaatca acgcccggct ggaggtggag cagctgtcga tgtttatcgc
    10021 cgagcacagc ttgaccggct ggtattgcgc ggtgcgtcag cccggccggc tgtggccggg
    10081 tgcgccggtg acgctggttt cccgcccggc cgacacgctg agcctggcgc aattcaacgc
    10141 cctgcaagcc gaacaccgcc ccgacccggc gcgcctgcgc acggtggctg ccatcgacgg
    10201 cctggcggcg gatgtggcgc agcggttgcg cgagcgggcg gcgtggctgg acggcttgcg
    10261 agcagggtga ca
//
LOCUS       DAOPYX010000081        10164 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_7716_length_10164_cov_7.644080, whole genome shotgun sequence.
ACCESSION   DAOPYX010000081 DAOPYX010000000
VERSION     DAOPYX010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 10164)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10164)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10164
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7716_length_10164_cov_7.644080"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            130..1062
                     /locus_tag="PKN55_05785"
     CDS             130..1062
                     /locus_tag="PKN55_05785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003823956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HrgA protein"
                     /protein_id="HNX70784.1"
                     /translation="MALNLRLRIIERLQALHGEPQTARELAEWLFASFPQECAAKKAA
                     SGARLQSDADLLQQLVAEIGSQRPHWQAKHPQLKTHESRPRRYYWTELDAAAEVAAAE
                     GVADPQAECTPVNEAVGEHALYPVLADYLRSEELGVWAMRINERRSSNRQGSGGNEWL
                     HPDLVGLEDLTAGWLPAVRECAGLLGEPRARLWSFEVKLLLNRSNARKSFFQAVSNSS
                     WAHLGYLVAERVEGDGALKELRMLSAAHGIGLIQLDRLNPSESQVLIPARERPNIDWE
                     MCHRLAAENPDFMEFLGRVRRFHQTGEVLPSEWR"
     gene            complement(1076..1678)
                     /locus_tag="PKN55_05790"
     CDS             complement(1076..1678)
                     /locus_tag="PKN55_05790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX70785.1"
                     /translation="MVATEDELSEAVALRLAAEVQPALTVTQTLRRQGFGYLRSKMAS
                     WYQLAQQQPVFLLTDLDRLACPATLLTQWLGALTPPPNLLMRVAVREVESWLLADHLA
                     MQQLLGEKGKLPPAPDSLDDPKQTLLTLAKKYAPRDVRLDLVQSTGAMACQGIGYNAR
                     LVDWVRTVWQPAQAATRSPSLHKARCRLQELAARCTSIPP"
     gene            complement(1687..3159)
                     /locus_tag="PKN55_05795"
     CDS             complement(1687..3159)
                     /locus_tag="PKN55_05795"
                     /inference="COORDINATES: protein motif:HMM:NF024700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNX70786.1"
                     /translation="MRVPREFESHRFRQIDKNPLIFQGVFVFSCSNALLLHLVRHLAK
                     MTVMAAMKVMALPLELLSAANKTFLALLPDLAVLPVLSAAARLARAATLLGFVGSAYC
                     SGCLSMQITRVKLKNWRNFRALDIPLHAVSYLLGPNAAGKSNLLDALRFLRDISKSKG
                     GGLQTAVAERGGISKLRCLHARSDNEVLIEVELGSTPDKPCEWRYVLGFKAEGKGAQR
                     ILISNEQVWKGETRLFARPDAQDQQDTMLLTQTRLEQIGANAEFRELADFFSATVYLH
                     LVPQLLKFSEKIGGQRLEDDPFGQGFLERLAKTNERTRHARLAKISEALKLAVPQFKE
                     LRFKKDELGHPHLEALYTHHRPNAGWQSEEHFSDGTLRLLGLLWSLLEGNALLLLEEP
                     EISLNDAIVKQIPLILDRMQRHRKTRRQIVVSTHSEALLSNPGIDGHSIIVLQSGANG
                     SEARTLDDEEAKLLRQGFSVAEVVLPKTRPQHVDQLGLSL"
     gene            complement(3119..3208)
                     /locus_tag="PKN55_05800"
     tRNA            complement(3119..3208)
                     /locus_tag="PKN55_05800"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3172..3174),aa:Ser,seq:tga)
     gene            complement(3302..4363)
                     /gene="rluD"
                     /locus_tag="PKN55_05805"
     CDS             complement(3302..4363)
                     /gene="rluD"
                     /locus_tag="PKN55_05805"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014087073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HNX70787.1"
                     /translation="MSHPYDNSEDYNDLSENRVVQIPTEFAGERLDAALAKLFPMYSR
                     SRLQQWIKDGHVLLNGEPALGKQKVYGDEQILLDAPPPPELTAFTAEEIYFPVVYEDE
                     HLLVIDKPAGLVVHPAAGNWSGTLLNGLLHYCPSQAQLPRAGIVHRLDKDTSGLMVVA
                     KTQLAQTDLVRQLQARTVKRHYRAVADGLVRQSGTIDAAIGRDPRNRLKMAVLSFGGK
                     PAITHVEVIERLQHHSLVECRLETGRTHQIRVHMQKAGHPLAGDPLYGNPRHAMSDAA
                     REAVNLLGRQALHAFRLALVHPATGETMQWKSRLPADMRALLTVLRSEMHGKALPLDD
                     DDFDDEDDGDWEIIYTREE"
     gene            4362..5156
                     /locus_tag="PKN55_05810"
     CDS             4362..5156
                     /locus_tag="PKN55_05810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003755523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HNX70788.1"
                     /translation="MKKIVAAMLMTAGLVACSSTKGPADETRGWPIERLYGEARDELN
                     SGNYTRAVQLYETLEARFPYGRYAQQAQMDLAYAHYKDKESQMALAACDRFIKLHPAH
                     ANLDYIYYLKGLIHFNEDESLLTRWTGQDLSERDPRAGREAFQAFGELIKRFPDSQYA
                     DDARKKMIILVDALAANEMHVARYYMKRKAYLAAANRAQAAIREFSNTPYVEEALALM
                     ATAYTELDLKPLADDSRRVLAQNYPNSRYLKTPWVYQDMPWYQFWR"
     gene            complement(5427..5924)
                     /locus_tag="PKN55_05815"
     CDS             complement(5427..5924)
                     /locus_tag="PKN55_05815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012695829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HNX70789.1"
                     /translation="MTEPLAALSADKNFPTVLVVYASQSGRTARLAQAAADGARQAEP
                     GARVWLRPALEARVEDLLAADALLLATPENFGYMAGALKDFFDRTYYPAQGRCQGLPY
                     AVMVCAGNDGRGAIAAIERIAIGYGWRAVAPPLRVLGEPDAQALADAAELGGGLAAGV
                     ALGIC"
     gene            5961..6269
                     /locus_tag="PKN55_05820"
     CDS             5961..6269
                     /locus_tag="PKN55_05820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017507104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2249 domain-containing protein"
                     /protein_id="HNX70790.1"
                     /translation="MQTSQIHLFDARGIAKRFRHAAIFGALESLSHGETMRFVNDHDP
                     LPLLGQIQQRYGEQIRLAYQQRDGEHVVIDFTVDLPGAEGSGGGCGGGGGGGCGCAGH
                     "
     gene            complement(6508..7452)
                     /locus_tag="PKN55_05825"
     CDS             complement(6508..7452)
                     /locus_tag="PKN55_05825"
                     /inference="COORDINATES: protein motif:HMM:NF019292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HNX70791.1"
                     /translation="MESVIDIILQAGRAAVELSLFVLLPVMIVMLAVMRLLEAYRVLD
                     WLVQRLSPLLRPFGLPGLGVFAALQINLVSFAAPVATLSLMEQRGASARHLAATLAMV
                     MAMAQANAAFPLAARGLQLGPTLLGSLLGGLLAAAITYHSFARHLSLDASAAAQTPPP
                     HGEADSAKDVLNVINRAGAEAFKIALGALPMLVLSLVAVTALTRLGAVEALTRLIGPG
                     LSALGIPPVLILPSLTKYLAGGNAMFGVMDALRGQGVVSAGLLNASAGWLIHPLDLPG
                     LAVLLSAGKRVAAVWRPAVLGALVGIAARTVWHVWLMA"
     gene            7697..8008
                     /locus_tag="PKN55_05830"
     CDS             7697..8008
                     /locus_tag="PKN55_05830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014086058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HNX70792.1"
                     /translation="MTVQTPPITPCASLDEVRHHIDRIDRQLVALIAERGGYVAQAAG
                     FKRSADDVAAPQRVAQVLDKVRALAQQQGASPEVVTAVWQAMIEAFIREEHAQWQAQA
                     K"
     gene            8142..9062
                     /locus_tag="PKN55_05835"
     CDS             8142..9062
                     /locus_tag="PKN55_05835"
                     /inference="COORDINATES: protein motif:HMM:NF025078.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4157 domain-containing protein"
                     /protein_id="HNX70793.1"
                     /translation="MNMKMHQPRQSAVQQASPRAHLSKAALAGPHHAQLQKLDTLAGQ
                     SAQLAQLHAVQKLADQRVRNQTGLPNQLKAGVEALSGISLDKVRVHYHSSKPAQLNAK
                     AYAQGTDIHLGPGQEKHLPHEAWHVVQQAQGRVKATAQMKGGVPINDDPALEHEADVM
                     GQKALQAAGMARAPALRALAGSSVQCVAQQTRDNFSARQRAKVLAKNRAKNNGFYTCE
                     HCGFQHKLLNYATYQGRRMGDGGFQVDHILHASRGGRALIGNARVLCGTCNTSRGNRA
                     MPSRFGIDKYRALHKKSFKKDYRRKPSKNR"
     repeat_region   9156..10143
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=9276..9303
                     /rpt_unit_seq="tttctcagctgcctattcggcagttcac"
BASE COUNT         2025 a         3138 c         3036 g         1965 t
ORIGIN      
        1 gtgagcggtg cagtcatgcc gccagcagca ggaacccacc gaagcgactc aggggtggcg
       61 caatgccgcg cctgagcgcc gtaggaatct ccggccttca ggcgggggag aatgtcaaaa
      121 taccttgata tggccctgaa tctacgtctg cgcatcatcg aacgcctgca agccctgcac
      181 ggcgaacccc aaaccgcccg tgagctggcc gaatggttat tcgccagctt tccgcaagag
      241 tgcgccgcca aaaaagccgc cagcggcgca cgtctgcaaa gcgatgccga cctgttgcaa
      301 cagctggtgg cggaaatcgg cagccagcgc ccgcactggc aagccaaaca tccgcaactg
      361 aaaactcacg aaagccggcc gcgtcgctat tactggacag aactggacgc cgccgccgaa
      421 gtggctgccg ccgaaggcgt ggctgatcca caggctgagt gcaccccagt caatgaagcc
      481 gttggcgagc acgcactcta cccggtattg gcggattatc tgcgtagcga agagcttggc
      541 gtgtgggcca tgcgcatcaa cgagcgccgc tcgtccaacc gtcagggcag cggcggcaat
      601 gaatggctgc accctgatct ggtggggctg gaagatctca ccgctggctg gctgccagcg
      661 gtgcgcgagt gtgccggcct gttgggcgaa ccgcgtgctc gcttgtggtc atttgaagtc
      721 aagctgctgc tcaaccgttc gaatgcgcgc aaaagttttt ttcaggcagt gtccaattca
      781 tcctgggcgc acctgggcta tttggtggcg gaacgggtgg agggcgacgg cgcattgaaa
      841 gaactgcgca tgctgtctgc cgcccacggc attggcctga ttcagctgga tcggcttaat
      901 ccatcagaaa gccaagtgct gattcccgct cgcgagcgcc ccaacatcga ctgggagatg
      961 tgccaccgcc tggcggccga aaaccctgat ttcatggagt ttcttggccg cgtgcgccgg
     1021 tttcaccaga ctggcgaagt gctgccaagc gagtggagat aagtcggcag cgcgtttacg
     1081 gcggtatgct tgtgcatctg gccgcgagtt cttgcaggcg acagcgggct ttgtgcaggc
     1141 tgggcgagcg cgttgccgcc tgggcgggtt gccagaccgt gcgcacccag tccaccaggc
     1201 gagcgttata gccgatgccc tggcaggcca tggcgccggt ggattgcacc aggtccaagc
     1261 gcacatcgcg cggcgcatat tttttggcca gcgtcagcaa ggtttgcttg gggtcgtcca
     1321 ggctgtcggg cgctgggggc aatttgccct tttcgcccag cagctgttgc atggcgaggt
     1381 gatcagccag caaccacgat tccacttcgc gcaccgccac acgcatcaac aggtttggcg
     1441 gcggagtgag agcgcccagc cattgagtga gcagggtggc cggacacgcc agccgatcca
     1501 gatcagtcaa caaaaatact ggctgctgct gagccaattg ataccagctc gccatttttg
     1561 agcgcaaata gccgaatccc tgccgacgca aggtttgcgt cactgtcagc gccggctgca
     1621 cttctgccgc caagcgcagg gcaacggctt cgctcaattc gtcttcagtg gccaccacca
     1681 gcccgctcat aaggacaggc ccagttgatc tacgtgctgt gggcgggttt ttggcaacac
     1741 cacctccgcc accgaaaatc cttgacgcag cagtttggct tcttcgtcat ccaaagtgcg
     1801 ggcttcgctg ccattggcgc cagattgcaa cacaataatg ctgtggccat caatgccggg
     1861 gttgctcagc aaggcttcgc tatgggtgct gaccacgatt tgacggcggg ttttgcggtg
     1921 acgctgcatg cgatcgagaa taagtggaat ttgtttgacg atagcgtcat tcaaagagat
     1981 ttccggctct tccagcaaca acagtgcatt gccctccagc aatgaccata acaatcccaa
     2041 cagccgcaag gtgccatccg agaagtgttc ttctgactgc cagcccgcat tggggcggtg
     2101 atgggtgtac aacgcttcca gatggggatg acccagttcg tcttttttga agcgtaattc
     2161 cttgaattgt ggcaccgcca gttttaatgc ttcgctgatc ttggccagcc tggcatgacg
     2221 ggtgcgttcg tttgttttcg ccaagcgctc aagaaagccc tggccaaatg ggtcgtcctc
     2281 cagacgttgg ccgccgattt tttcggagaa tttcagcaac tgtggcacaa ggtgtaagta
     2341 caccgtagcg ctgaaaaaat ccgccaactc gcgaaactcg gcattggcgc caatttgctc
     2401 cagccgggtt tgcgtcaata gcatggtgtc ttgttgatct tgtgcatccg ggcgagcaaa
     2461 caggcgcgtc tcgcctttcc agacttgctc attggaaatc aaaatgcgct gagcgccctt
     2521 gccttcggct ttgaatccca gcacatagcg ccattcacag ggcttgtcgg gtgttgaccc
     2581 caactcaact tcgatcagca cttcattatc gctacgcgca tgcaggcaac gcagtttgct
     2641 gatgccgcca cgctcagcca cggcggtttg cagcccacct cccttggatt tgctgatgtc
     2701 gcgcaaaaac cgcagggcat cgagcagatt ggacttgccc gcggcattgg ggccgagcaa
     2761 atatgaaact gcatgcaggg gaatatccaa tgcacggaag ttgcgccagt ttttcagttt
     2821 cactcgggtg atctgcatcg ataggcatcc tgaacaataa gcgctgccca caaaacccag
     2881 cagcgtggcg gccctggcta ggcgtgcagc cgcagacagt acgggtagta cggcaaggtc
     2941 gggcaacaag gccaggaagg ttttattagc cgcgcttagc aactctaaag gcaatgccat
     3001 caccttcatg gcagccataa ctgtcatttt ggccagatgc cgcacaagat gcaacaacag
     3061 ggcattgcta catgaaaaaa caaaaacccc ctgaaaaatc agggggtttt tatcgatttg
     3121 gcggaagcgg tgagattcga actcacgggg gactcgcatc cccgacggtt ttcaagaccg
     3181 ctgccttaaa ccgctcagcc acgcttccgg aacccataac tataccggct tgccgggctt
     3241 ttgcctagcc ccgatgccat gcggatggct ggcgctggcc ggggtcagcg ccggcttggg
     3301 cttactcttc gcgggtgtaa ataatctccc agtcgccatc atcctcatcg tcaaagtcgt
     3361 cgtcgtccag cggcagcgcc ttgccgtgca tttcgctgcg cagcacggtg agcagggcgc
     3421 gcatatcggc gggcaggcgg gatttccact gcatggtttc gccggtggcc gggtgcacca
     3481 gggccaagcg gaaggcgtgc agcgcctggc ggcccagcag gttgaccgct tcgcgggcgg
     3541 cgtcgctcat ggcgtggcgc gggttgccat acagcgggtc gccggccagc gggtgcccgg
     3601 ctttttgcat gtgcacgcga atctggtggg tgcggccggt ttccagtcgg cattccacca
     3661 ggctgtggtg ttgcaggcgc tcgatcacct ccacatgggt gatggccggt ttgccgccaa
     3721 agctgagcac ggccattttc aggcggttgc gcgggtcgcg gccgatggcg gcgtcaatcg
     3781 tgccggattg gcgcaccagg ccgtcggcca cggcgcggta gtggcgcttg acggtgcgcg
     3841 cttgcagctg gcgcaccagg tcggtttgcg ccagttgggt ttttgccacc accatcaggc
     3901 cgctggtgtc tttgtccagc cggtgcacaa tgccagcgcg cggcagttgg gcctggctgg
     3961 ggcagtagtg cagcaggccg ttgagcaagg tgcccgacca gttgccggcg gccgggtgca
     4021 ccaccaggcc ggcgggtttg tcgatcacca gcaggtgctc gtcttcgtac accaccggaa
     4081 aataaatctc ttcggcggta aaggcggtca gctcgggcgg cggcggggcg tccagcagga
     4141 tttgctcgtc gccgtagact ttttgcttgc ccagcgccgg ctcgccattg agcaagacgt
     4201 ggccgtcctt gatccattgt tgcagccggg agcgcgagta catcgggaac agtttggcca
     4261 gtgcggcgtc gagccgctct ccggcgaatt ccgtcggtat ctggacgaca cggttttcgg
     4321 ataagtcgtt ataatcttcg gaattgtcgt aaggatggct catgaaaaaa atcgtagctg
     4381 caatgctgat gacagcaggc ctggtggctt gttcgtccac caagggacct gcagatgaaa
     4441 cgcgaggctg gccgattgaa cggctgtacg gcgaagcccg cgacgagttg aacagcggca
     4501 attatacccg tgccgtgcaa ctctatgaaa cgctggaagc acgctttccg tacggtcgct
     4561 atgcccaaca ggcccaaatg gacctggctt acgcccatta caaggataaa gagtcgcaaa
     4621 tggcgctggc cgcctgcgat cgctttatca agctgcaccc ggcgcacgcc aacctggatt
     4681 acatctatta cctgaagggc ttgatccact tcaacgaaga cgaatcgctg ctgacccgct
     4741 ggaccgggca ggatttgtcc gagcgtgacc cgcgcgccgg ccgtgaggct ttccaggcat
     4801 ttggcgagct gatcaagcgc ttcccggata gccagtatgc ggacgacgcc cgcaagaaga
     4861 tgatcattct ggtggatgca ctggccgcca acgagatgca tgtggcgcgc tactatatga
     4921 agcgcaaggc ctatctggcg gcggccaacc gcgcccaggc cgccattcgg gaattctcca
     4981 acacgccgta cgtggaagaa gccttggcgc tgatggccac tgcttacacc gagctggatc
     5041 tcaagccgct ggccgacgac agccgccgcg tgctggcgca aaactacccg aacagccgct
     5101 acctgaaaac cccgtgggtg tatcaggaca tgccgtggta tcagttctgg cgttaagagc
     5161 cgctgacaaa accctctggc gttgtggtgt gaccttgccg tactactacc cgtactgtct
     5221 gcggtcacac gcctagccag gaccgctgcg ctgggttttg tgagcaactc taagcggagc
     5281 aagcgacggc agactttgtg tctgacagaa aacagggcca acgctgccaa ggcgctggcc
     5341 ctgttttttt agcgctgctc acaaaattca gcgaaacgtt cctgaaaggc aggccccgtg
     5401 cctgcccttg ttgcgtctgg cgcgggctag cagatgccca gggccacccc ggccgccagg
     5461 ccgccgccca gttcggcggc gtcggccagc gcttgcgcat cgggctcgcc cagcacacgc
     5521 aagggcggtg ccacggcgcg ccagccgtag ccaatggcaa tgcgctcaat agcggcaatc
     5581 gcgccacggc cgtcattgcc ggcgcacacc atcactgcat agggcaggcc ctggcagcgg
     5641 ccttgcgccg ggtaataggt gcggtcaaaa aaatctttca gcgcgccggc catatagcca
     5701 aagttctctg gcgtggccag cagcagcgca tcagcggcca gcaaatcctc cacgcgggct
     5761 tccagcgccg ggcgcagcca cacccgcgca ccgggctcgg cctggcgcgc gccatcggcg
     5821 gcggcctggg ccaggcgggc ggtgcggccg ctctggctgg cgtacaccac cagcacggtc
     5881 gggaaattct tatccgcgct caaggcggcc aaaggctcgg tcatggcaca gtgccccctg
     5941 tgtttaccgg agaatcccgc atgcaaacct cgcaaattca tctgttcgac gcgcgtggca
     6001 tcgccaagcg ctttcgccac gcagccattt ttggcgcgct ggaatcgctc agccatggtg
     6061 aaaccatgcg ctttgtgaat gatcatgatc cgctgccgct actggggcaa atccagcagc
     6121 gctacggcga gcaaatccgc ctggcctatc agcagcgcga tggcgaacac gtggtgattg
     6181 atttcaccgt ggacttgccc ggcgcagaag gctcgggcgg cggttgtggt gggggtggtg
     6241 gcggcggttg cggctgcgcc ggccactgag cggcggcgtt ttggcgccac gccttgcttt
     6301 agcgtagcgc ctgtacgcca gatcacggtg tgtgtttcag gccccaaatg gggtctgaat
     6361 catttgcgcg cgactgagca ctgttcaacc aaacccagca cagcgcgcct ggcgaggcgc
     6421 gcggccacag acagtacaag tcgtacggct aaggtcatgc aacaacgcca ggagggtttt
     6481 gttaaccact cttaaccccc catggtgcta cgccatcagc cacacatgcc acaccgtgcg
     6541 tgcggcaatg cccaccagcg cacccagcac cgccggccgc cacacggcgg ccacccgttt
     6601 accggccgac aacagcaccg ccaggccggg caaatccagc gggtgaatca gccagccggc
     6661 actggcattc agcaaaccgg cgctgaccac gccctgacca cgcagcgcat ccatcacccc
     6721 aaacatggca ttaccgcccg ccaggtattt ggtcaggctg ggcaaaatca gcactggcgg
     6781 aatacccagc gcactcaggc cggggccgat cagccgggtg agcgcctcga ccgcgcccaa
     6841 gcgggtgagc gccgttaccg ccaccagcga cagcaccagc atcggcaaag cgcccaaggc
     6901 gatcttgaac gcctcggcgc cagcgcgatt gatcacattc aacacgtctt tggcgctgtc
     6961 tgcctcgcca tgcggcggcg gtgtctgggc tgcggctgac gcatccaggc tcaagtggcg
     7021 ggcaaaacta tggtaggtga tggctgccgc cagcaagccg cccagcagcg aacccagcaa
     7081 ggtgggcccc agctgcaagc cacgcgccgc caacggaaac gccgcattgg cctgcgccat
     7141 ggccatcacc atcgccagcg tggcggccag atgacgggca ctggccccgc gctgctccat
     7201 caagctcagc gtggccactg gcgcggcaaa gctcaccaga ttgatctgca aagcggcaaa
     7261 cacccccagc cccggcaagc caaacgggcg caacagcggg ctgagccgct gcaccagcca
     7321 atccagcaca cggtaagcct ccagcagccg catcaccgcc agcatgacaa tcatcacggg
     7381 cagcaacaca aacaaagaca gctccaccgc cgcgcggcca gcctgcaaaa taatgtcgat
     7441 gacggattcc atggcggcga ttatgacatt gtttagccct ggcgttggcg cttgacaccc
     7501 acgccatccc gccggaatac tgcgccaatg acctataccc gatttgccca cgcccccatt
     7561 gccgcccgcc tgatttgctg gtggcgcgcc gccgtgcgtc cgtggcctgc ttgaggtatt
     7621 gctgagtttt tcgtcaacag gccacgctga gcgcgtggcc tgttttttta gcgccgccca
     7681 tacagaaaga acccagatga cagtccaaac accaccgatt accccctgcg cctcattgga
     7741 cgaagtgcgc caccacatcg atcgcatcga ccgccaactg gtggcgctga ttgccgagcg
     7801 cggcggctat gtcgcccagg cggcaggctt caagcgcagc gccgacgacg tggccgcccc
     7861 gcaacgggtg gcgcaggtgc tggacaaagt gcgcgccctg gcgcaacaac aaggcgcctc
     7921 gcccgaggtg gtgaccgcag tatggcaggc gatgattgaa gcgtttatcc gcgaggaaca
     7981 cgcgcagtgg caggcgcagg caaaataacc gccgctcaca aaacaccctg gcgttgtggt
     8041 gtgaccttgc cgtactctgc gagtactgtc ttgcggccgc acatttggcc aggatcgctg
     8101 cgctgggtgt agtcggccgt tcttaaacaa ggattttcat catgaacatg aaaatgcacc
     8161 aaccccgtca atcggcggta cagcaagcgt cgccacgcgc ccacctcagc aaggccgcgc
     8221 tggccggccc ccatcacgcc cagctgcaaa aactggatac cctggccggg caatccgccc
     8281 agttggccca attgcacgca gtgcaaaagc tggcggacca gcgcgtgcgc aatcaaaccg
     8341 gcttgccgaa tcaactcaag gcaggggtag aagccttgtc tggcatatcg ctggacaaag
     8401 tgcgcgtgca ttaccattcc agcaaacccg cgcaactcaa cgccaaggcc tatgcccagg
     8461 gcacggacat tcacctgggc cccggccagg aaaagcacct gccgcatgaa gcctggcatg
     8521 tggtgcaaca ggcgcaaggc cgggtgaagg ccacggcgca aatgaagggc ggcgtgccaa
     8581 tcaacgacga cccggcgctt gaacacgaag ccgatgtgat ggggcaaaaa gctcttcagg
     8641 cagcgggaat ggcgcgcgca cctgcgctgc gggcattagc cggcagcagc gtgcagtgcg
     8701 ttgcccagca aacgcgagac aatttttccg ccaggcagcg cgccaaagta ctggcaaaaa
     8761 accgggctaa aaacaatggt ttttatacct gcgaacactg cgggtttcag cacaaactgc
     8821 tgaattacgc cacctaccaa gggcggcgca tgggcgacgg cggttttcag gtagaccaca
     8881 tcctgcatgc ctcacgcggc ggccgcgcgc tgattggcaa cgcccgcgta ctgtgcggca
     8941 cctgcaacac ctcgcgcggc aaccgcgcca tgcccagccg gtttggcatc gataaatacc
     9001 gcgccctgca taaaaaatcg tttaaaaaag actatcgcag gaaacccagt aaaaaccgat
     9061 aggtgtggat gttttgagca gaatgcatgc ctcttggcgc cgcgctgaag cgtgctttac
     9121 atcccgcatg tcgtcagcgc ggtggaatag aatggggggg cagcagcttg ttcggcagtt
     9181 cactcgtgct gtacgccggc gcaggaaccg atatctttct tagctgccta ttcggcagtt
     9241 cacgccggcg cgccggctgg cggtggcggt gatcttttct cagctgccta ttcggcagtt
     9301 cacggtgatg ctcaccgacc cggtgataac tgccatttct cagctgccta ttcggcagtt
     9361 cacttattcg caatgcggtg ggtgcgcagt cggcttttct cagctgccta ttcggcagtt
     9421 cacggccagt gatattgccg catggctcgc agaattttct cagctgccta ttcggcagtt
     9481 cacaccaaag aagcgctggc gcggaaaaaa gccgatttct cagctgccta ttcggcagtt
     9541 cacgatcgcc aggcgctcag cgtcagtatt ggtcatttct cagctgccta ttcggcagtt
     9601 cacggcagcg caccgaaaat ggcctggcgg atgcctttct cagctgccta ttcggcagtt
     9661 cacgagcggg ccggtttgct atctgctgct gataatttct cagctgccta ttcggcagtt
     9721 cactgcgtca ggcccaggcg ctgatgcgtg agcattttct cagctgccta ttcggcagtt
     9781 cacggctcgc atcgtccgcg ctgggcacac gttcctttct cagctgccta ttcggcagtt
     9841 caccgtgggg gcagatgatg tgcggtgcac gggtgtttct tagctgccta ttcggcagtt
     9901 cacctgcaca tcggcggcag ctgggcggcc ggtcatttct cagctgccta ttcggcagtt
     9961 cactgtcgga ctggtccgag tttctggacg gttactttct cagctgccta ttcggcagtt
    10021 cactgtcgcg cagcctgggc gccagcaact agctctttct cagctgccta ttcggcagtt
    10081 caccgggttt tgccggtggg cagtgcgcgg tacattttct cagctgccta ttcggcagtt
    10141 caccattttt gacatctccg agct
//
LOCUS       DAOPYX010000082        10163 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_7719_length_10163_cov_14.213593, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000082 DAOPYX010000000
VERSION     DAOPYX010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 10163)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10163)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10163
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7719_length_10163_cov_14.213593"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(211..585)
                     /gene="trxA"
                     /locus_tag="PKN55_05840"
     CDS             complement(211..585)
                     /gene="trxA"
                     /locus_tag="PKN55_05840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012696076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HNX70794.1"
                     /translation="MAVLELTADTFNDAIAADKTVIIDFWAPWCGPCRSFAPTFEAAA
                     AKHTDVVFAKINTEEQQQLAGELNIRSIPTLMVFREQVLLFNQAGALSAGQLETLLQQ
                     VAELDMAQIHAEIAAREADNQG"
     gene            764..1912
                     /locus_tag="PKN55_05845"
     CDS             764..1912
                     /locus_tag="PKN55_05845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003782263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="THUMP domain-containing protein"
                     /protein_id="HNX70795.1"
                     /translation="MHTDRFFAPCPRGLEGVLAEELDTLGASAIETADGGVAFAGDFT
                     VMMRANLHSRFASRILWRLGGGRCRTEAELYRAASGIDWPLLFNVERTIKVKTDAIRS
                     PLKSLEFASLKIKDAVCDVFRQRTGQRPSVDTRAPDVRIHAFLTADTATLYLDTSGEA
                     LFKRGWRRETQEAPLRENLAAGILRLSGWQPGEPLLDPMCGSGTFLVEAADMALNRAP
                     GRARTFGFEKLNGFVPARWRTLQDEAHAAERPAAPLPIWGSDRYRPALDSARQNLEAA
                     GLTGCVQLDCRDVLESRPPAEHGVVVCNPPYGVRLDDQDRLAELYPRLGDWLKQHWSG
                     WNAFFFTADLRLAKLIRLSASRRTPLFNGALECRLFAYKMVAGGNRKA"
     gene            complement(1985..3937)
                     /locus_tag="PKN55_05850"
     CDS             complement(1985..3937)
                     /locus_tag="PKN55_05850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012695581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HNX70796.1"
                     /translation="MNVFYEEDGGFKVGAVMSSTDASLQVEAPHGKRSKIKTAHVLLK
                     FTSPLADFLPQAETAAADIDVDFLWECCGADEFQFEALAADYWGHAPSAIEAAAIALR
                     LHGAPMYFYRKGRGHYKAAPEDTLKAALAGQEKRRQQQLQIDEWAAQLRAGNIPEALR
                     SQLMTLLHRPDKNTLEWKALDAASRDANQPPLRLVAAAGGIPSVPDYLLAGFLMEHFP
                     KGRGFSAYAPAQAPGELVDAGVQAFSIDDAATTEIDDAFSLTRLDNGNWRVGVHIAAP
                     TLGIGVDSELEKLVYNRLSTVYFPGDKITMLPDDAVNAFTLKEGQHCPAVSMYLEVAP
                     DFTVLSHESRVEQVFVAANLRHDTLEPLFNEDTLANDPGVDYPYKAELRWLWEFANAL
                     EVRRGKADPTRPPQLDYNFAIEDGKVIITRRKRGAPMDKLVSELMILVNCEWGGELDR
                     AGFPAIYRAQTQGKVKMVTTAQPHVGLGVAQYAWSSSPLRRAVDFVNQRQLVAMIRGE
                     PAAFPPGDARLYAILRDFDAAYSAYNQFQDQMERFWCLRWFTQEGVSEPIATYVKEDL
                     VRIDGLPMATRLSGLPDGLQAGDKVRLAIVRIDELTLDIEYRVLGRLDGAAPEPVALD
                     DAVDDVADTEAASVAEEVSATDGAAS"
     gene            4256..4789
                     /locus_tag="PKN55_05855"
     CDS             4256..4789
                     /locus_tag="PKN55_05855"
                     /inference="COORDINATES: protein motif:HMM:NF016214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HNX70797.1"
                     /translation="MASRKAHHASGVAAGVLAALLVVRAGAGGPFQLWAIVALFAGAW
                     GGTAPDWLEVAWWRSRGQRGAQRLWIKHRTLTHWGVPWVLLLCGSYISLGSAWWAPAA
                     FGFAAGGVMHLLADWPNPMGVPWLFRRHSLAMWTSGSFEVLIIVLSWAACALLADGML
                     FGGQHTGNMFRALSTLI"
     gene            complement(4969..5394)
                     /locus_tag="PKN55_05860"
     CDS             complement(4969..5394)
                     /locus_tag="PKN55_05860"
                     /inference="COORDINATES: protein motif:HMM:NF017103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DciA family protein"
                     /protein_id="HNX70798.1"
                     /translation="MSASPFTDIASRDARLKRLFAAVRLNQRATAVMQGALPPGLAGL
                     FRVVRIEQGEVLVYARNGGAATKLRLLADHLIHALRQRGLDVGALRVKVHVDEPEPPR
                     PAKQIALTPHARESLSRAAAQIGSPALRAAMERLARNEG"
     gene            5591..8341
                     /gene="secA"
                     /locus_tag="PKN55_05865"
     CDS             5591..8341
                     /gene="secA"
                     /locus_tag="PKN55_05865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HNX70799.1"
                     /translation="MISSLLKKVFGSRNDRLVKQYRQTVARINALESGLTGLSDEALG
                     AKTAEFRQRLANGETLEQLLPEAFAVCREASRRALGMRHFDVQLIGGMVLNDGKIAEM
                     RTGEGKTLVATLPAYLNALTGNGVHIVTVNDYLASRDAALMAPLYEFLGLSVGVNLSQ
                     MSHEAKQAAYGADITYGTNNEFGFDYLRDNMVFSLAEKVQRKLAFAVVDEVDSILIDE
                     ARTPLLISGPADDNVEMYQRMNGVPAMLTRQTEEEGSGDYWVDEKAHTVMLSEAGHER
                     AEAILTELGLLAEGDSLYSATNITLMHHLMAALRAHALYHRDQHYVVQNDEVVIVDEF
                     TGRLMSGRRWSEGLHQAVEAKEGVTINRENQTLASITFQNYFRLYRKLSGMTGTADTE
                     AYEFQQIYGLETVVIPTNKPMIRKDNLDLVYRTNPEKYRAIVADIRDCQQRGQPVLVG
                     TTSIESSELLSNFLKQEKLAHEVLNAKQHAREADIVAQAGRAGAITIATNMAGRGTDI
                     VLGGSIASELKALAADDSLSEAERASRTAALKAEWQQRHDAVLAAGGLHIIGTERHES
                     RRIDNQLRGRSGRQGDPGSSRFYLSLEDPLLRIFASERVSAIMERLKMPEGEAIEHTW
                     VTRAIENAQRKVEGRNFDIRKQLLEYDDVANDQRKVIYQQRNDILDTPDVSDMVANMR
                     QAALGALFDLYLPPGSIEEQWDAAGLEKALEAEYQLQAPLSEWMKAAPDAELDTLREQ
                     LIALGEAHYQAKVDLAGETTMRQFEHAVLLQHLDQHWREHLAALDHLRQGIHLRGYAQ
                     KNPKQEYKREAFELFSHMLDSIKRDVAQLLFTVRIQSAEEVAAMEAEEQRQRPMQFQH
                     ADFDAALAADVEALASASLPGDDNPFSPEALAAQGLRVGRNDPCPCGSGKKYKQCHGK
                     LG"
     gene            8748..9722
                     /locus_tag="PKN55_05870"
     CDS             8748..9722
                     /locus_tag="PKN55_05870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012696660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNX70800.1"
                     /translation="MRTSIRLSLLAASLLAAPLALANPDVVRLGNLKFAHYGAVSYMK
                     DYCGKYNLKVEERMFPKGPDIMPAIVAGEIDVAALASDGAISGRANGVPIYTVAGFAK
                     GGARIVAGVDSGIKNIQGLKGKKVGVTRGGAHELLLYAELEKAGLSWSDKPGKDVQIV
                     FLAFADLNQALAAKQIDAMCQSEPQSSQAINKKFGVEIMKPYTTKMGEPVRLLVMTEK
                     LYNEKKDVAQRLMKCFVDSTAQFNKDSALAEKYVREQMFKGQITPQDFKDAMDNADYT
                     YDVSLEHVDITTDYMQKYGVGRMAKPPKAAEWVKLDLLQKAKAELKVK"
     gene            9730..>10163
                     /locus_tag="PKN55_05875"
     CDS             9730..>10163
                     /locus_tag="PKN55_05875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014086029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNX70801.1"
                     /translation="MNKLKSLMHGALAPVLVLLVWEACARAGLFSAVVMPAPSAVAAK
                     WWAYLLPAQPQAEGQSYLGWLLSGELLHDAYSSLYRVIAGFVIGAGLALPLGLLMGAN
                     TRIHDFFNPLVQILRPIPPIAYIPLAILWFGLGNPPSFFLIA"
BASE COUNT         1804 a         3220 c         3342 g         1797 t
ORIGIN      
        1 gcgcgccgca gtagcagcaa cagccgcacc acccgaaccg ccagccgcag cgcagtaccg
       61 gccacgcctc aagtcaaagc cgtcccggcc gccgccccgg tcagcgaaca ggattttgaa
      121 cgcttctgac ggcgtagcgc ccgcccataa aaaaaccgcc gagtcactcg gcggtttttt
      181 tatgggcggc aagcttaagc agcctcaggc tcagccctga ttgtctgctt cacgggcggc
      241 gatttccgcg tgaatctgcg ccatatccag ctcggccact tgttgcaaca gcgtttccag
      301 ctggccagcc gacagcgcgc cggcctgatt gaacagcagc acctgctcgc ggaacaccat
      361 cagcgtcggg atcgaacgga tgttcagctc gccggccagt tgctgctgtt cttcggtgtt
      421 gatcttggcg aacaccacat cggtgtgctt ggccgctgcg gcctcaaacg taggggcaaa
      481 gctgcggcag gggccgcacc acggcgccca gaaatcaatg atcacggttt tgtcggcggc
      541 gatggcgtcg ttgaaagtat cagcggtgag ttcgagaacg gccatgatgg cttccttctt
      601 ggcgggggca ataccagcag ggtagcggaa tccggccggg gcggtgatgg tatttgcctg
      661 tcacggcgat agttttacgc tatgaccgac cggccgggca aaagccggct acaattcagc
      721 ctgattgtct acacgggcgc aggtcgccca cagccgggat tccatgcata ccgaccgctt
      781 ttttgcccct tgtccgcgcg gactggaagg ggtgcttgcc gaagaactgg acacgctggg
      841 cgcgtccgcc atcgaaaccg ccgacggcgg cgtggccttt gccggtgatt tcaccgtgat
      901 gatgcgcgcc aatctgcact cgcgctttgc cagccgcatt ctgtggcggc tgggcggcgg
      961 gcgttgccgc accgaggccg agctgtaccg cgccgccagc gggatcgact ggcccttgct
     1021 gtttaatgtc gagcgcacca tcaaggtcaa gaccgacgcc atccgcagcc cgctgaaaag
     1081 cctggagttc gcctcgctga aaatcaagga cgcggtgtgc gatgtgttcc gccagcgcac
     1141 cggccagcgc cccagcgtgg acacccgcgc gccggacgtg cgcattcacg cctttctgac
     1201 cgccgacacc gccacgctct acctggacac ctcgggcgag gcgctgttca agcgcggctg
     1261 gcggcgggaa acccaggaag ccccgctgcg ggaaaacctg gccgccggca ttctgcgcct
     1321 gagcggctgg caaccgggcg agcccttgct cgacccgatg tgcggcagcg gcacgttttt
     1381 ggtggaagcc gccgacatgg cgctgaaccg cgcgccaggc cgcgcccgca catttggttt
     1441 tgagaagctc aatggctttg tgccggcgcg ctggcgcacg ctgcaagacg aggcgcacgc
     1501 cgccgagcgg ccggccgcgc cgctgccgat ctggggcagc gaccgctacc gcccggcgct
     1561 ggacagcgcc cggcaaaacc tggaagccgc cgggctgacc ggctgcgtgc agctggactg
     1621 ccgcgacgtg ctggaaagcc gcccgccggc cgagcacggc gtggtggtgt gcaacccgcc
     1681 ctacggcgtg cgactggacg accaggaccg cctggccgag ctgtacccgc gcctgggcga
     1741 ctggctgaaa cagcattggt cgggctggaa tgcgtttttc tttaccgccg acttgcggct
     1801 ggccaagctg atccgcctgt cggccagccg acgcacgccc ttgttcaatg gcgcgctgga
     1861 gtgccggctg tttgcctaca agatggtggc cggcggcaac cgcaaagcgt aagcgccgct
     1921 cagccgcccc gccggggcgg gtgcgctgtc cggggtctac cgggcagcgc cattcacaca
     1981 cgcttcaaga cgccgcgcca tctgttgcgc tgacctcttc ggccacactg gcggcctcgg
     2041 tgtcggcaac atcgtccacc gcgtcgtcca gcgccaccgg ctcgggcgcg gcgccgtcca
     2101 gccggcccag cacgcggtat tcgatatcca gggtcaattc gtcgatgcgc acaatcgcca
     2161 gtcgcacctt gtcgccggct tgcaggccgt cgggcaggcc ggacaggcgg gtggccattg
     2221 gcaggccgtc gatgcgcacc aggtcttcct tgacgtaggt ggcaatcggc tcgctgacgc
     2281 cttcctgggt gaaccagcgc aggcaccaga agcgctccat ttggtcttgg aactggttgt
     2341 aggcgctgta ggcggcgtca aagtcgcgca ggatggcgta cagccgggcg tcgccaggcg
     2401 ggaaggcggc cggttcgccg cgaatcatcg ccaccagctg gcgctggttg acaaagtcca
     2461 ccgcgcggcg cagcggcgag ctggaccagg cgtattgcgc cacgcccagg ccgacgtgcg
     2521 gctgggcggt ggtgaccatt ttcaccttgc cctgggtttg cgcgcggtaa atcgccggga
     2581 agccggcgcg atccagctcg ccgccccatt cgcagttgac cagaatcatc agctcggaca
     2641 ccagcttgtc catcggcgcg ccgcgcttgc ggcgggtgat gatgaccttg ccgtcttcga
     2701 tggcgaagtt gtaatccagc tgcggcggcc gggtcgggtc ggccttgccg cgccgcactt
     2761 ccagcgcgtt ggcgaattcc cacagccagc gcagctcggc cttgtacggg taatccacgc
     2821 cggggtcgtt ggccagggtg tcttcgttga acagcggctc cagcgtgtcg tggcgcaggt
     2881 tggcggcgac aaacacctgc tcgacccggc tttcgtggct gagtacggtg aaatccgggg
     2941 cgacttccag atacatgctc accgccgggc agtgctggcc ttctttcagg gtgaaggcgt
     3001 tcaccgcgtc gtccggcagc atggtgatct tgtcgccggg gaagtacacg gtggacaggc
     3061 ggttatacac cagtttttcc agctcgctgt ccacgccaat gcccagcgtc ggcgcggcga
     3121 tgtgcacgcc cacgcgccag ttgccgttgt ccaggcgggt gagcgagaag gcgtcgtcga
     3181 tttcggtggt ggcggcgtcg tcaatcgaaa acgcctgcac gccggcatcg accagctcgc
     3241 cgggggcttg cgccggggca taggccgaaa acccccggcc cttggggaag tgctccatca
     3301 ggaaaccggc cagcagatag tccggcaccg acggaatgcc gccagcagcg gccaccaggc
     3361 gcaggggcgg ctggttggcg tcgcggctgg cggcgtccag ggctttccat tccagggtgt
     3421 ttttgtccgg gcggtgcaag agcgtcatca gctggctgcg cagcgcctcg gggatgtttc
     3481 cggcgcgcag ctgggcggcc cattcgtcga tttgcagctg ctgctggcga cgtttttcct
     3541 ggccggccag cgcggccttg agggtgtctt ccggcgcggc tttgtagtgg ccacggccct
     3601 tgcggtagaa atacatcggc gcgccatgca ggcgcagggc gatggcggcg gcctcgatgg
     3661 ccgacggcgc atggccccag taatcggcgg ccagcgcttc aaactggaat tcatccgcgc
     3721 cgcagcattc ccacaggaaa tccacgtcga tgtccgccgc ggcggtttcg gcctgcggca
     3781 aaaagtccgc cagcggcgaa gtgaacttca gcagcacatg ggcggttttg attttgctgc
     3841 gcttgccgtg cggcgcttcg acttgcaggc tggcgtcggt ggaggacatg acggcgccaa
     3901 ccttgaagcc gccgtcttct tcgtaaaaca cattcatcgt gggagtcccg cgcgggagag
     3961 tggctagccc atcagtatac cagcagcgct gtggggctta gcgccgctga caaaactcgg
     4021 caaagcggcc ctggccaggc gcgatgaccg cacacagcac aaggcgtagg gcaaggccgg
     4081 acaatgacgc cgaaagggtt tggtgagcgg ctattactga acggtgacac ccgcgggcga
     4141 tgtcagcact ggccagacgc gcgtcttggc cgttgagatt ttcgggtatc atgcgccatt
     4201 cctccggcca actggccggt ggcaatcaat tgagttgtct ggagttttgc agagtatggc
     4261 gtctagaaaa gcgcatcacg cctcaggcgt ggccgctggg gtgctggcgg cgttgctggt
     4321 ggttcgcgcc ggggcgggcg ggccgtttca gctgtgggcg atcgtggcct tgtttgccgg
     4381 ggcctggggc ggcaccgcgc cggattggct ggaggttgcg tggtggcgca gccggggcca
     4441 gcgcggcgca cagcgtttgt ggatcaaaca ccgcacgctg acgcactggg gcgtgccctg
     4501 ggtgttgctg ctgtgcggct cgtatatcag cctgggcagc gcctggtggg cgccagcggc
     4561 atttggcttt gctgctggcg gagtgatgca cctgctggcc gactggccca atccgatggg
     4621 ggtgccgtgg ctgtttcgcc gccattcgct ggccatgtgg accagcggca gttttgaggt
     4681 gctgattatt gtcttgtcgt gggcggcgtg cgcgctgctg gccgatggca tgctgtttgg
     4741 cggccagcat accggcaata tgttccgtgc cttgtctacc ttgatctgaa cacgtcccgg
     4801 cgatgcttaa gcgcggctaa caaaaccctc ctggcgttgt ggcacgacct tgccggacga
     4861 ctcccgtact gtccgcggtc gtgcgcctgg ccagggcggc tgtgccgggt tttgtgagcg
     4921 gcgcttatta tactcacgcc cggcctgcgc cgggcgtttt gcatgcgctc agccctcgtt
     4981 gcgcgccagc cgctccatgg cggcgcgcag ggcaggactg ccgatttgcg ccgccgcgcg
     5041 gctgagcgat tcgcgtgcgt gtggggtcag cgcaatctgc ttggccgggc gcggcggctc
     5101 gggctcgtcc acatgcactt tcacccgcag cgcgcccacg tccaggccgc gttggcgcag
     5161 ggcgtgaatc aggtggtcgg ccaagaggcg cagcttggtg gcggcgccgc cgttgcgggc
     5221 gtacaccagc acttcgccct gctcgatgcg caccacgcga aacagcccgg ccaggccggg
     5281 cggcagcgcg ccctgcatca ccgccgtggc gcgctggttc agccgcacgg cggcaaacag
     5341 gcgcttgagc cgggcgtcgc ggctggcaat gtcggtgaac gggctggcgc tcatgggggg
     5401 gcttgaaaaa ggcgggagat gggtcgattt tagcatggct gcgcgcgccg gcccgagctt
     5461 ggcgcatggg gccggcccgg ccgcccagcg cttgcccctt tagactttgt ggtcattcac
     5521 gccatcccgg ctcacccggc tgtgctagaa ttttcagaat ttttttacgg tctctggccg
     5581 aaagccttcc atgatttcct ccctgctcaa aaaagtgttc ggcagccgca acgaccggct
     5641 ggtcaagcaa tatcggcaaa ccgtggcgcg catcaatgcg ctggagagcg gcctcaccgg
     5701 cctgagcgac gaggcgctgg gcgccaagac ggcggagttc cgccaacgcc tggccaatgg
     5761 cgaaacactg gagcaactgc tgccagaagc ctttgccgtt tgccgtgaag ccagccgccg
     5821 cgcgctgggc atgcgccatt tcgacgtgca gctgattggc ggcatggtgc tcaacgacgg
     5881 caagattgcc gaaatgcgca ccggcgaagg caaaacactg gtggccaccc tgccggccta
     5941 cctgaatgcg ctgaccggca acggcgtgca catcgtcacc gtcaacgact acctggccag
     6001 ccgcgatgcg gcgctgatgg cgcccttgta tgagttcctg ggcctgagcg tgggggtgaa
     6061 cctgtcgcag atgtcgcacg aggccaaaca agcggcctat ggcgccgaca tcacctacgg
     6121 caccaataat gaattcggct ttgactacct gcgcgacaat atggtgttca gcctggccga
     6181 gaaggtgcag cgcaagctgg cctttgccgt ggtcgatgag gtggactcga tcttgattga
     6241 cgaggcgcgc acgccgctgc tgatttctgg cccggccgac gacaatgtcg agatgtacca
     6301 gcgcatgaac ggcgtgccgg ccatgctgac ccggcaaacc gaggaagaag gctcgggcga
     6361 ttactgggtg gacgaaaaag cccacacggt gatgctctca gaagccggcc acgagcgcgc
     6421 cgaagccatc ctcaccgagc tgggcctgct ggcggagggc gacagcctgt attcggccac
     6481 caacatcacc ctgatgcacc acctgatggc ggcgctgcgc gcacatgcgc tgtatcaccg
     6541 cgaccagcac tatgtggtgc aaaacgacga agtggtgatt gtcgatgaat tcaccggccg
     6601 gctgatgtcc gggcggcgct ggagcgaggg cctgcatcag gcggtggaag ccaaggaagg
     6661 cgtcaccatc aaccgcgaaa accagacgct ggcgtcgatc accttccaga attacttccg
     6721 gctgtaccgc aagctgtcgg gcatgaccgg caccgccgac accgaagcct atgaattcca
     6781 gcagatttac ggcctggaaa ccgtggtgat ccccaccaac aagccgatga tccgcaaaga
     6841 caatctggac ttggtgtacc gcaccaaccc ggaaaaatac cgcgccatcg tcgccgacat
     6901 ccgcgactgc cagcagcgcg gccagccggt gctggtgggc accacctcga ttgaaagctc
     6961 cgagctgctg tccaacttcc tcaagcagga aaaactggcg cacgaagtgc tgaacgccaa
     7021 gcagcacgcc cgcgaggcgg atattgtggc gcaggccggc cgcgccggcg ccatcaccat
     7081 cgccaccaat atggccggtc gcggcaccga catcgtgctg ggcggcagca tcgccagcga
     7141 gctgaaggcg ctggcggcgg acgacagcct gagcgaagcc gagcgcgcca gccgcaccgc
     7201 cgcgctgaag gccgaatggc agcagcgcca cgacgccgtg ctggccgctg gcggcctgca
     7261 catcatcggc accgaacgcc acgaatcgcg ccgcatcgac aatcagctgc gcggccgctc
     7321 cggccgtcag ggcgaccccg gctccagccg cttctacctg agcctggaag acccgctctt
     7381 gcgcattttt gcctcggagc gcgtgtccgc catcatggaa cggctgaaaa tgccggaagg
     7441 cgaggccatc gagcacacct gggtgacccg cgccatcgaa aacgcccagc gcaaggtgga
     7501 aggccgcaac tttgacatcc gcaagcaatt gctggaatac gacgacgtcg ccaacgacca
     7561 gcgcaaggtg atttaccagc agcgcaacga cattctggac accccggatg tgtcggacat
     7621 ggtggccaat atgcgccagg cggcgctggg cgcgctgttt gatctgtatc tgccgccggg
     7681 ctcgattgaa gagcaatggg acgccgccgg cctggaaaaa gcgctggaag ccgaatacca
     7741 gctgcaagcg ccgctcagcg agtggatgaa ggccgcgccg gacgccgagc tggacaccct
     7801 gcgcgaacag ctgatcgccc tgggcgaggc gcactaccag gccaaggtgg atctggctgg
     7861 cgaaaccacc atgcgtcagt ttgaacacgc cgtgctgctg caacatctgg atcagcactg
     7921 gcgcgaacac ctggccgcgc tggaccacct gcgccagggc attcacctgc gcggctacgc
     7981 gcaaaagaac cccaagcagg aatacaagcg cgaggcattc gagctgttct cgcacatgct
     8041 ggattcgatc aagcgcgatg tggcccagct gctgttcacc gtgcgcattc aaagcgccga
     8101 agaagtggcc gccatggaag ccgaagagca gcgccagcgg ccgatgcagt tccagcatgc
     8161 tgattttgac gcagcgctgg cggccgatgt cgaggcgctg gccagcgcca gcctgccggg
     8221 cgacgacaac ccgttcagcc cggaagcgct ggccgcacaa ggcctgcgcg tgggccgcaa
     8281 cgacccgtgc ccgtgcggct caggcaagaa gtacaagcag tgccatggca agctgggtta
     8341 agcctcgcta agctgactct gcactgaacc cacaggccgc cggcaatccc ggcggcctgt
     8401 ggcgtttctg cacgttgctt tccggttcaa cccctcccca ctgtgcggaa atccgcacag
     8461 ccgttttctg catctggcga tattccgcac gccggctttc tgcgcaaagc aataaatcca
     8521 ttaaattcag gtgtttattc ttgctctgtg catggcacgc ttcctgccaa ggtagtggag
     8581 cacagcagcg ccgctgcgcc ctgtgcagag ccgctcacaa aactctcctg gcgtcgttgc
     8641 tcggccttgc cgtactcctt gtactgtctg cggccgcgcg cctagccagg agcgcttcgc
     8701 tgagttttgt gagcggcgct taaaccaaaa tcagaggaga aaccaccatg cgcacatcca
     8761 tccgcctgtc cctgctggcc gccagcctgc tggccgcccc cttggcgctg gccaaccccg
     8821 acgtggtgcg tctgggcaat ctgaaatttg cccattacgg cgcggtgtcc tacatgaagg
     8881 attactgcgg caagtacaac ctgaaggtgg aagaacgcat gttccccaag ggcccggaca
     8941 tcatgccggc gattgtcgcc ggcgagatcg acgtggccgc gctggcctcg gacggggcga
     9001 tttctggccg cgccaatggc gtgccgattt acaccgtggc cggttttgcc aagggcggcg
     9061 cgcgcatcgt ggctggggtg gacagcggca tcaagaacat ccaggggctg aagggcaaaa
     9121 aggtgggcgt cacccgtggc ggcgcacatg agctcttgct gtacgccgag ctggaaaaag
     9181 ccggcctgag ctggtcggac aagccgggca aggatgtgca gattgtgttt ttggcctttg
     9241 ccgacctgaa ccaggcgctg gcggccaagc agatcgacgc catgtgccag tccgagccgc
     9301 aatcctcgca ggcgatcaat aaaaaattcg gcgtggaaat catgaagccg tacaccacca
     9361 agatgggcga gccggtgcgc ctgctggtga tgacggaaaa actctacaac gagaaaaaag
     9421 acgtcgccca gcgcctgatg aagtgttttg tcgatagtac ggcgcagttc aacaaagata
     9481 gcgcgctggc agagaaatac gtgcgcgagc agatgttcaa gggccagatc accccgcagg
     9541 acttcaaaga cgccatggac aatgccgact acacctacga tgtgtcgctg gagcacgtcg
     9601 acatcaccac tgattacatg cagaaatacg gcgtcggccg catggccaag ccgcccaagg
     9661 cggcagagtg ggtcaagctg gatctgctgc aaaaggccaa ggccgagctg aaggtgaagt
     9721 gagacggcca tgaacaaact caaatcgctg atgcatggcg cgctggcccc ggtgctggtg
     9781 ttgctggtgt gggaggcctg tgcgcgcgcc gggctgtttt cggccgtggt gatgccggcg
     9841 ccgtcggcag tggcggccaa atggtgggcg tatttgctgc cagcgcagcc gcaggccgag
     9901 ggccagtcgt atctgggctg gctgttgtcg ggcgaattgc tgcacgacgc gtattccagc
     9961 ctgtaccggg tgattgccgg ctttgtgatt ggcgccgggc tggcgctgcc gctgggcttg
    10021 ctgatgggcg ccaacacccg catccatgac tttttcaacc cgctggtgca aatcctgcgc
    10081 ccgattccgc cgattgctta cattccgttg gccatcttgt ggtttggcct gggcaacccg
    10141 ccgtcgttct ttttgatcgc cat
//
LOCUS       DAOPYX010000083        10147 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_7738_length_10147_cov_12.938169, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000083 DAOPYX010000000
VERSION     DAOPYX010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 10147)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10147)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10147
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7738_length_10147_cov_12.938169"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(160..1188)
                     /locus_tag="PKN55_05880"
     CDS             complement(160..1188)
                     /locus_tag="PKN55_05880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX70802.1"
                     /translation="MSSHKERFADKMEFPPATPQPNVYPLSWHSKTKKLQEVWEAALR
                     ENWDPEKLPWDTLDVDSYSWEERESIAYWWTLLSVFDASAPPVFAEALIKTYEVHEED
                     PVRKCFFSVTRDEQNHEIMCGLAITKMLGHPDPLTYTPKTELGKRLQKNVQWLYFNGG
                     RYWTGYKQAVPKYDLAVLFSSFLMGEIAAATIFKQMFDNSREAVFKEGFKNIGRDEGR
                     HMAICMAVMERDYPGLDESTKAVVTKQIRAGYLFLSAVLFEPPMEFWDLPSDFIANQR
                     EAEEVARGAGFGIPTYEAKLENWKNAMINLKGVLDRYNIPFPAIPEVGITGQEVSEID
                     MEDIIPVF"
     gene            1528..3195
                     /gene="paaN"
                     /locus_tag="PKN55_05885"
     CDS             1528..3195
                     /gene="paaN"
                     /locus_tag="PKN55_05885"
                     /inference="COORDINATES: protein motif:HMM:TIGR02288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation protein PaaN"
                     /protein_id="HNX70803.1"
                     /translation="MTHPLFEQHRATLEGALNAIATRGYWSAYAEMPSPKLYGEHAAD
                     AGKRAFEAHLGQRFALEQPGQTGWQGGERSPYGVALEVSYPVCAPEALLAAAQAAMPG
                     WQALGAAGRTGVCLEMLARLNAQSFELAHAVMMTTGQGWMMAFQAGAPHAQDRGLEAV
                     AYAWREQSFIPTRAHWEKPQGKNPALVMEKHFEIVGRGVALVIGCATFPTWNTYPGLF
                     AALATGNPVIIKPHNQAILPAAITVRTLRAVLAEHGLDPNLVSLCVSPSRAATQALAR
                     DPAVRSVDFTGGNAFGQWLLQHCPQAQVYAELAGVNNIVIDSTDQYKAMLGNLAFTLS
                     LYSGQMCTTSQAILVPAGGIDTDEGHKSFDEVCADLARAVQRLLGKPEVAHAVLGAIQ
                     SADTLARIEQANRGELGQVLLAASALDNPEFPAAQVRTPALLACDAADEALYMEERFG
                     PISFVVKTADTAASLALCERLVSQHGALTVGVYSTREDIIDAATAVTWRGKVALSINL
                     TGGVFVNQSAAFSDYHGTGGNPAANAAYANSAFVANRFCVVQRRRHV"
     gene            3206..4000
                     /gene="paaG"
                     /locus_tag="PKN55_05890"
     CDS             3206..4000
                     /gene="paaG"
                     /locus_tag="PKN55_05890"
                     /EC_number="5.3.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019923196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-(1,2-epoxy-1,2-dihydrophenyl)acetyl-CoA
                     isomerase PaaG"
                     /protein_id="HNX70804.1"
                     /translation="MGFQHILFEARDGVARLTLNRPDALNSLTDAMHIELRAALDQVQ
                     ADPAIRVLVLSGAGRGFCAGQDLADPGMQAVDGKLPDLGDIVERNYKPLVLRLQNLRV
                     PTVAAVNGVAAGAGASLALACDLVVAAESASFLQAFSKIGLIPDTGATWALPQRVGMA
                     RAMGLALLADKLPAARAAEWGLIWACVADGEFRAHVDALAARLAAMPTKALVRTRLAM
                     HTAASHTLEQQLTLEGGLMRELGWSADYAEGVAAFVEKRAPRFSGA"
     gene            3997..5526
                     /locus_tag="PKN55_05895"
     CDS             3997..5526
                     /locus_tag="PKN55_05895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019923195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase"
                     /protein_id="HNX70805.1"
                     /translation="MNMAALSALQPVGVVGAGAMGAGIAQVAALAGHPVRLFDLRADA
                     ARQAVDSQRAQLAQLAGKGRLDAALAEAASARLSAVSELAELAGCALVIEAIVEQLEA
                     KQALFRQLEAIVGEHALLASNTSSISLTAIAAPLTRPQRLAGLHFFNPAPRMALVEVI
                     AGLATAPEVIDTLMATARAWGKTPVRAKSTPGFIVNRVARPYYAEALRLAQEGAASPA
                     TLDALLREAGGFRMGPFELMDMIGHDVNFAVTSSVWRGYFHDPRFLPSLMQQDLVEAG
                     FLGRKRGRGFYDYRDGAAMPQADSAPPLPPPAQLAVCGDSPAARALAARLHAHGVAFA
                     ALPSVDGRVAQADDAVLFVTDGRSASQRAADLALPNLLLIDLALDYHLAPRLAAAAAD
                     GCAPAARDAGLGLLQAAGLAVSLLDDAPGLVVMRTVAMLANEAADAVHQGVCSAADAD
                     AAMQLGVNYPRGPLAWAEQIGLAQVRQVLAHLAACYGEDRYRISPRLQRAALTGRILH
                     D"
     gene            5519..5977
                     /gene="paaI"
                     /locus_tag="PKN55_05900"
     CDS             5519..5977
                     /gene="paaI"
                     /locus_tag="PKN55_05900"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019923194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyphenylacetyl-CoA thioesterase PaaI"
                     /protein_id="HNX70806.1"
                     /translation="MTDIAALSPQALAEAAAAAMWRRDAAARSLGMQLEAISPGSATL
                     SMTVRADMVNGHHICHGGMLFSLADTAFAYACNSGNHNTVASACQIDFLAPAHEGDTL
                     IASASEQAASGRAGVYDVSVHRSDGQLLALFRGKSHRIRGEVIAGLQAAG"
     gene            6044..7366
                     /gene="paaF"
                     /locus_tag="PKN55_05905"
     CDS             6044..7366
                     /gene="paaF"
                     /locus_tag="PKN55_05905"
                     /EC_number="6.2.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019923193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047475 - phenylacetate-CoA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HNX70807.1"
                     /translation="MTARLPQPGELDPIETASRDEIAALQLQRLKWSLRHCYDHVAPY
                     RQRCQAKGVHPDDLKQLSDLSLFPFMTKSDLRDHYPFGLLAVPRERIARLHASSGTTG
                     KSVVVGYTQQDLHHWADLVARSIRAAGGRPGDMVHVAYGYGMFTGGLGAHYGAERLGC
                     TVVPMSGGQTEKQVQQIVDFRPNIIMVTPSYSLVIAEEFERLGIAPQDISLKVGIFGA
                     EPWGEGMRNEIEKKLGIDAIDIYGLTEVMGPGVACECIESKDGPVIWEDHFYPEIIDP
                     DTGEVLPDGSEGELVFTSLTKEAFPVIRYRTRDLTRLLPPTSRAFRRIDKITGRSDDM
                     LIIRGVNVFPTQIEEQILRDARLSGNYQVVVSRDGHLDNLEVRCELQRELSGRLNPAQ
                     VQEIAKSLQHRIKTIIGVSTRISVLEVDGIPRTVVGKARRVIDERPKQ"
     gene            7377..8582
                     /gene="pcaF"
                     /locus_tag="PKN55_05910"
     CDS             7377..8582
                     /gene="pcaF"
                     /locus_tag="PKN55_05910"
                     /EC_number="2.3.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008952950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0019619 - 3,4-dihydroxybenzoate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoadipyl-CoA thiolase"
                     /protein_id="HNX70808.1"
                     /translation="MNQAFICDAVRTPIGRYGGALAGVRADDLGAAPLQALMARHPQL
                     DWAAVDDILYGCANQAGEDNRNVARMSALLAGLPIQAPGTTINRLCGSGMDAIGQAAR
                     AIKAGEAELMLAGGVESMSRAPFVLAKADSAFSRQAAIYDTTIGWRFANPRMKALYDT
                     HSMPQTADNVAAEFAISRADQDAFAWRSQQRWAAADAAGRFADELIPLRIPQKKGEPT
                     TVTRDEHPRPDTTLEQLARLKGVNGPELSVTAGNSSGVNDGACALLLASAAAAQRHGL
                     TPLARVVGMATAGVPPRIMGFGPAPAVKKVLAHTGLTLADMDVIELNEAFAAQALAVL
                     RDLGLADDASHVNPNGGAIAMGHPLGMSGARLVTTAAYELRRRGGRYALCTMCIGVGQ
                     GIAMVIERV"
     gene            8621..>9206
                     /locus_tag="PKN55_05915"
     CDS             8621..>9206
                     /locus_tag="PKN55_05915"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNX70809.1"
                     /translation="MQASIRNTLFGLALAVAAGHASGAEPIKIGSVLSVTGPAAFLGD
                     PELKTLQMYVEDINKKGGVLGRPLQLVHYDDGSDANKANGFAKRLIDDDKVDVLIGGT
                     TTGSTMSMVPLVERAGLPFISLAGAVPIIEPVKKWVFKTPHTDRMAAEKVFEDMKKRG
                     LSKVALLSETSGFGQSGKKETEAVAGKYGVTLVAS"
     assembly_gap    9207..9216
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <9217..9772
                     /locus_tag="PKN55_05920"
     CDS             <9217..9772
                     /locus_tag="PKN55_05920"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNX70810.1"
                     /translation="PKDTDMSPQLTKIKNTPGVQALFIFGLGQGPAIATKNYKQLGLT
                     LPLYHAHGVASEEFIKLAGPAAEGVRLPATALLVIDKLDAKDPQKPVVTNYARAFQAR
                     WKTDVSSFGGYAYDGLMLTVDAIKRAGSADKAKVRDALEATKGYVGASGKVNMSASDH
                     MGLDLSAFRLVEIKAGDWALLGSK"
     gene            9783..>10147
                     /locus_tag="PKN55_05925"
     CDS             9783..>10147
                     /locus_tag="PKN55_05925"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HNX70811.1"
                     /translation="MTDSVLTETHGRVGLIRFNRPEAMNALNADVVAALAAAIDDFEA
                     NPDIGCLVLTGSDTVFAAGADIGFMKDFSYLHAYRTDFVTTGGWERLKTTRKPVIAAV
                     AGFALGGGCELAMMCDMIFA"
BASE COUNT         1709 a         3383 c         3353 g         1692 t
ORIGIN      
        1 cgtaggcgca atcgacggac ttgcccgagg gttgcatgat gatgcgagcc attttgtctc
       61 cccctaatgg gtgttggttt ctctgttgcg gcaaggccga cgccctgatg gggcgccggc
      121 cggagcaatt actgcaggaa ttgcggcttc atccggtgtt tagaagaccg ggatgatgtc
      181 ttccatgtcg atctcggaca cttcctggcc ggtgatgccg acttccggaa tcgccgggaa
      241 cgggatgttg tagcggtcga gcacgccctt caggttgatc atggcgttct tccagttttc
      301 cagcttggcc tcgtaggtcg ggatgccgaa gccggcgccg cgcgccactt cctcggcttc
      361 gcgctggttg gcgatgaagt cggacggcag gtcccagaat tccatcggcg gctcgaacag
      421 cacggcggac aggaacaggt agccggcgcg gatctgcttg gtgacgacgg ccttggtgga
      481 ctcgtcgagg cccgggtagt cacgctccat gaccgccatg cagatggcca tgtggcggcc
      541 ttcgtcgcgg ccgatgttct tgaagccttc cttgaagacg gcctcgcggg aattgtcgaa
      601 catctgcttg aagatggtgg cggcagcgat ttcgcccatc aggaaggagc tgaacaggac
      661 agccaggtcg tacttgggca cggcctgctt gtagccggtc cagtagcggc cgccgttgaa
      721 atacagccac tgcacgttct tctgcaggcg cttgccgagc tcggtcttcg gcgtgtaggt
      781 cagcgggtcc gggtggccga gcatcttggt gatggccagg ccgcacatga tctcgtggtt
      841 ctgctcgtcg cgggtcaccg agaagaagca cttgcgcacc gggtcttctt cgtgaacttc
      901 gtaggttttg atcagcgctt cggcaaatac cggcggcgcc gaggcgtcga acaccgacaa
      961 cagcgtccac cagtaggcga tggattcgcg ctcttcccag ctgtagctgt ccacatccag
     1021 ggtgtcccag gggagttttt ccgggtccca gttctcacgc agcgcggctt cccacacttc
     1081 ttgcaatttt ttggtcttgc tgtgccagga cagcggatag acgttgggct gcggggtggc
     1141 cggcggaaat tccatcttgt cggcaaagcg ttccttgtgg ctggacatgg cgtctcctca
     1201 tctgtggtgg gtggtctgca agcagtgtcc cgtctgcttg tggcggcaat agtgatacgt
     1261 aaaaaaatac atgtcaacaa aaaatgtatc gctttgtgtg gggtgagtgt gtgggctgtc
     1321 ggcgcaacac tggcgtatgc tgcgcaaaaa cagcagaaaa ttgcctatta aattattgat
     1381 aaaaaacaga ttacgattaa caaaattcgt ttttacatgg tgaaatgtgc gcgcgcagca
     1441 agaaaaaata tgatacgtaa agttgacaca aagcgagttt gttcgtaact ttttcgcgaa
     1501 cacacccact ttgcggagac tgaaccgatg actcaccccc tgtttgagca gcatcgcgcc
     1561 accctggaag gcgcgctgaa cgccatcgcc acccgtggct actggtcggc ctatgccgag
     1621 atgcccagcc ccaagctgta tggcgaacac gccgccgacg ccggcaagcg cgcgttcgaa
     1681 gcgcatctgg gccagcgctt tgccctggag cagccgggcc agaccggctg gcagggcggc
     1741 gagcgctcgc cgtatggcgt ggcgctggag gtgagttatc cggtgtgcgc gcccgaggcg
     1801 ctgcttgccg ccgcgcaggc ggccatgcct ggctggcagg cgctgggcgc cgccgggcgc
     1861 actggcgtgt gtctggaaat gctggcgcgg ctgaatgcgc aaagctttga actggcccac
     1921 gcggtgatga tgaccaccgg ccagggctgg atgatggcgt ttcaggccgg cgcgccgcat
     1981 gcccaggatc gcgggctgga ggcggtggcc tacgcctggc gcgagcaaag cttcatcccg
     2041 acgcgcgcgc actgggaaaa accgcagggc aagaacccgg cgctggtgat ggaaaaacac
     2101 tttgaaatcg tcggccgtgg cgtggcgctg gtgattggct gcgccacctt ccccacctgg
     2161 aacacctacc ccggcctgtt tgccgcgctg gccaccggca acccggtgat catcaaaccg
     2221 cacaaccagg ccatcctgcc ggccgccatc accgtgcgca ccctgcgcgc cgtgctggcc
     2281 gaacacgggc tggacccgaa tctggtcagc ctgtgcgtca gcccctcgcg cgccgccacc
     2341 caggcgctgg cccgcgatcc ggcggtgcgc tcggtggatt tcactggcgg caacgccttt
     2401 ggccaatggc tgctgcaaca ctgcccgcaa gcccaggtgt acgccgagct ggccggggtc
     2461 aacaacatcg tcatcgactc caccgaccaa tacaaagcca tgctgggcaa tctggccttt
     2521 accctgtcgc tgtattccgg gcagatgtgc accaccagcc aggccatcct cgtgccggcg
     2581 ggcggcatcg acaccgacga aggccacaaa agctttgatg aggtgtgcgc cgacctggcc
     2641 cgcgccgtgc agcgcctgtt gggcaagccg gaagtcgccc acgccgtgct gggcgccatc
     2701 cagtcggccg acaccctggc gcgcatcgaa caagccaatc gcggcgagct gggccaggtg
     2761 ctgctggccg ccagcgcgct ggacaacccg gaattccccg ccgcccaggt gcgcacgccg
     2821 gccttgctgg cctgcgacgc cgccgacgag gcgctgtaca tggaagagcg cttcggcccg
     2881 atcagctttg tggtcaaaac cgccgacacc gccgcctcgc tggcgctgtg cgaacggctg
     2941 gtcagccagc atggcgcctt gaccgtcggc gtgtactcca cccgtgaaga catcatcgac
     3001 gccgccaccg ccgtcacctg gcgcggcaag gtggcgctgt cgatcaacct cactggcggc
     3061 gtgttcgtca accagtcggc ggcgttctcc gattaccacg gcacgggcgg caacccggcg
     3121 gccaacgccg cctacgccaa ttcggccttt gtcgccaacc gcttctgcgt tgtgcaacgc
     3181 cgtcgccatg tctgaggaac cggccatggg ctttcaacac attctgtttg aggcgcgcga
     3241 cggcgtggcg cggctgacgc tgaaccgccc ggacgcgctc aacagcctga ccgacgccat
     3301 gcacatcgag ctgcgcgccg cgctggatca ggtgcaggcc gacccggcca ttcgcgtgct
     3361 ggtgctcagc ggcgccgggc gcggcttttg cgccgggcag gatttggccg atccgggcat
     3421 gcaggcggtg gacggcaagc tgcctgacct gggcgacatc gtcgagcgca actacaaacc
     3481 cttggtgctg cgcctgcaaa acctgcgcgt gcccaccgtg gcggcggtca atggcgtggc
     3541 ggcaggcgcc ggggcgtcgc tggcgctggc ctgcgacctg gtggtggcgg cggaatccgc
     3601 gtcgtttctg caagcattca gcaagatcgg cctgattccc gacaccggcg ccacctgggc
     3661 gctgccgcaa cgggtgggca tggcccgcgc catgggcctg gccttgctgg ccgacaaact
     3721 gccggcggcg cgcgccgccg agtggggctt gatctgggcc tgcgtggccg atggcgagtt
     3781 tcgcgcccat gtcgatgcgc tggccgcgcg cctggcggcc atgcccacca aggcgctggt
     3841 gcgcacccgg ctggccatgc acaccgccgc cagccacacc ctggagcaac agctcaccct
     3901 ggaaggcggg ctgatgcgcg agctgggctg gagcgccgac tacgccgaag gcgtggccgc
     3961 ctttgtggaa aagcgcgcgc cgcgctttag cggggcatga atatggctgc tttgtctgct
     4021 ttgcagccgg tgggcgtggt cggcgccggg gcgatgggcg ccggcattgc ccaggtggcg
     4081 gcgctggccg gccacccggt gcgcctgttt gacctgcgcg ccgacgccgc ccgccaggcg
     4141 gtggacagcc agcgcgccca gctggcgcaa ctggccggca agggtcggct ggacgccgcg
     4201 ctggccgagg ccgccagcgc gcggctgtcg gcggtgagcg agctggccga gctggccggc
     4261 tgcgcgctgg tgatcgaagc gattgtcgaa cagctggagg ccaaacaggc gctgttccgc
     4321 cagctggaag ccatcgtcgg cgagcacgcc ctgctggcca gcaacacctc gtcgatttcg
     4381 ctgaccgcca tcgccgcgcc gcttacccgc ccgcagcggc tggccggttt gcattttttc
     4441 aacccggcgc cgcgcatggc gctggtggaa gtgattgccg gcctggccac cgcgcccgag
     4501 gtgatcgaca cgctgatggc caccgcccgc gcctggggca aaaccccggt gcgcgccaaa
     4561 tccacgccgg gttttatcgt caaccgggtg gcgcggccgt attacgccga ggcgctgcgc
     4621 ctggcgcagg aaggcgcggc cagcccggcc acgctggatg cgctattgcg cgaggcgggc
     4681 ggttttcgca tggggccgtt cgagctgatg gacatgatcg gccacgacgt caactttgcc
     4741 gtcacctcgt cggtgtggcg cggctatttt catgacccgc gttttttgcc ttcgctgatg
     4801 cagcaagacc tggtcgaggc gggctttttg gggcgcaagc gcggacgtgg gttttatgac
     4861 taccgcgacg gcgccgccat gccgcaggcc gacagcgcgc cgccgctgcc gccgcccgct
     4921 cagctggcgg tgtgcggcga cagcccggcc gcgcgcgccc tggccgcccg gctgcacgcc
     4981 cacggcgtgg cctttgccgc gctgccctct gtcgatggcc gggtggccca ggccgatgac
     5041 gccgtgttgt tcgtcaccga tggccgcagc gccagccagc gcgccgccga cctggcgctg
     5101 cccaatctgc tgctgatcga cctggcgctg gattaccacc tggccccgcg cctggccgcc
     5161 gccgccgccg atggctgcgc gccggccgcg cgcgacgccg ggctgggctt gctgcaagcc
     5221 gccggcttgg ccgtcagcct cttggacgac gcccccggcc tggtggtgat gcgcacggtg
     5281 gccatgctgg ccaacgaagc cgccgacgcc gtgcatcagg gcgtgtgctc ggccgccgac
     5341 gccgacgccg ccatgcaatt gggcgtcaac tacccacgcg gcccgctggc ctgggccgag
     5401 cagattggcc tggcgcaggt gcgccaggtg ctggcccatc tggccgcctg ctacggcgaa
     5461 gaccgctacc gcatttcgcc gcgcctgcaa cgcgcggcgc tcaccgggag aatcctgcat
     5521 gactgacatc gccgccctgt cgccccaggc gctggccgaa gccgccgccg ccgccatgtg
     5581 gcgccgcgac gccgccgccc gcagcctggg catgcaactg gaagccatca gccccggcag
     5641 cgccacgctg agcatgaccg tgcgcgccga catggtgaac ggccaccaca tctgccacgg
     5701 cggcatgctg ttcagcctgg ccgacaccgc ctttgcctac gcctgcaaca gcggcaacca
     5761 caacaccgtc gcctcggcct gccagattga ttttctggcg ccggcgcacg aaggcgacac
     5821 gctgatcgcc agcgccagcg aacaggccgc cagcggccgc gctggcgtgt acgacgtcag
     5881 cgtgcaccgc agcgacggcc agttgctggc gctgtttcgc ggcaagagcc atcgcattcg
     5941 cggcgaggtg attgccggtt tgcaggccgc cggctaacgc cgcagacaac agagcgcggc
     6001 tcaccgcgcc atgcccccta cacagacatc aggagacttc accatgaccg cccgtctgcc
     6061 ccagcccggc gagctcgacc cgatcgagac cgccagccgc gacgaaatcg ccgccctgca
     6121 attgcaacgc ctgaaatggt cgctgcgcca ctgctacgac catgtcgccc cctaccgcca
     6181 gcgctgccag gccaaggggg tgcacccgga tgacctgaaa cagctgtcgg atttgtcgct
     6241 gtttccgttc atgaccaaat ccgacctgcg cgaccactac ccgtttggcc tgctggcggt
     6301 gccgcgcgag cgcatcgccc gcctgcacgc ctccagcggc accactggca aatcggtggt
     6361 ggtgggctac acccagcagg atttgcacca ctgggccgac ctggtggccc gctcgatccg
     6421 cgccgctggc gggcggcctg gcgatatggt gcatgtggcc tacggctacg gcatgtttac
     6481 cggcggcctg ggcgcgcatt acggcgccga gcggctgggt tgcaccgtgg tgccgatgtc
     6541 cggcggccag accgaaaaac aagtgcagca gattgtcgac ttccgcccga acatcatcat
     6601 ggtcaccccg tcgtattcgt tggtgattgc cgaagagttc gagcgcctgg gcatcgcccc
     6661 gcaggacatt tcgctgaagg tgggcatttt tggcgccgag ccgtggggcg agggcatgcg
     6721 caacgaaatc gagaaaaaac tcggcatcga cgccatcgac atctacggcc tgaccgaagt
     6781 catgggcccc ggcgtggcct gtgagtgcat cgaaagcaag gacggcccgg tgatctggga
     6841 agaccatttc taccccgaga tcatcgaccc ggacaccggc gaagtgctgc ccgacggcag
     6901 cgaaggcgag ctggtgttca cctcgctcac caaagaagcc tttccggtga ttcgctaccg
     6961 cacccgcgac ctcacccggc tgctgccgcc cacctcgcgc gcgttccgcc gcatcgacaa
     7021 gatcaccggc cgctcggacg acatgctgat tattcgcggc gtgaatgtgt tccccaccca
     7081 gatcgaagag caaatcctgc gcgacgcccg cctgtcgggc aattaccagg tggtggtcag
     7141 ccgcgatggc catctggaca acctggaagt gcgctgcgag ctgcaacgcg agctgtccgg
     7201 ccggctgaat ccggcccagg tgcaggagat cgccaagagc ttgcagcacc gcatcaagac
     7261 cattatcggc gtctccaccc gcatcagcgt gctggaagtg gacggcattc cgcgcaccgt
     7321 ggtgggcaag gcccgccggg tgattgacga acggcccaag caataaggag cggcccatga
     7381 accaggcttt tatctgtgac gccgtgcgca ccccgattgg ccgctatggc ggcgccttgg
     7441 ccggcgtgcg cgccgacgac ctgggcgcgg cgcccttgca ggcgctgatg gcgcgccacc
     7501 cgcagctgga ttgggcggcg gtggacgaca ttctgtatgg ctgcgccaac caggccggcg
     7561 aggacaaccg caatgtggcg cgcatgtcgg cgctgctggc cgggctgccc atccaggcgc
     7621 cgggcaccac catcaaccgc ctgtgcggtt cgggcatgga cgccattggc caggcggcgc
     7681 gggcgatcaa ggccggcgaa gccgagctga tgctggccgg cggggtggaa agcatgtcgc
     7741 gcgcgccgtt tgtgctggcc aaggccgaca gcgcgttttc gcgccaggcg gcgatttacg
     7801 acaccaccat cggctggcgc tttgccaatc cgcgcatgaa ggcgctgtac gacacccact
     7861 cgatgccgca aaccgccgac aacgtggccg ccgaattcgc catcagccgc gccgaccagg
     7921 atgcctttgc ctggcgcagc cagcagcgct gggccgccgc cgacgccgcc gggcgctttg
     7981 ccgacgagct gattccgctg cggattccgc agaaaaaagg tgagcccacc acggtgacgc
     8041 gcgacgagca cccgcgcccg gacaccacgc tggaacaact ggcgcggctc aagggcgtca
     8101 acggcccgga actgagcgtc accgccggca attcgtctgg cgtcaacgac ggcgcctgcg
     8161 cgctgttgct ggcctcggcc gccgccgccc agcgccatgg cctgaccccg ctggcgcggg
     8221 tggtgggcat ggccaccgcc ggcgtgccgc cgcgcatcat gggctttggc ccggccccgg
     8281 cggtgaaaaa agtgctggcg cacaccggcc tgacgctggc cgacatggac gtgatcgaac
     8341 tcaacgaagc ctttgccgcc caggcgctgg ccgtgctgcg cgacctgggg ctggccgacg
     8401 acgccagcca cgtcaacccc aatggcggcg ccatcgccat gggccacccg ctgggcatga
     8461 gcggcgcccg gctggtgacc accgccgcat atgaactgcg ccgccgtggc ggccgctacg
     8521 cgctgtgcac catgtgcatt ggcgtgggcc agggcatcgc catggtgatc gagcgggtgt
     8581 gaccgcgctt taccccataa ccatcaggag agaccacatc atgcaagcat ccatccgcaa
     8641 caccctgttt ggcctggcgc tcgccgtggc tgccggtcac gccagcggcg cagagccgat
     8701 caaaatcggc tcggtgctgt cggtcaccgg cccggcggcg tttttgggcg accccgagct
     8761 gaaaaccctg caaatgtacg tcgaggacat caacaaaaaa ggcggcgtgc tgggccggcc
     8821 cttgcagctg gtgcactacg acgacggcag cgacgccaac aaggccaatg gttttgccaa
     8881 gcggctgatc gacgacgaca aggtggacgt gctgattggc ggcaccacca ctggctcgac
     8941 catgtcgatg gtgccgctgg tcgagcgcgc cggcctgccg ttcatctcgc tggccggcgc
     9001 ggtgccgatt atcgaaccgg tgaaaaaatg ggtgttcaaa accccgcaca ccgaccgcat
     9061 ggccgccgaa aaagtctttg aagacatgaa aaagcgcggc ctgagcaagg tggcgctgct
     9121 gtcggaaacc tcgggctttg gccagtcggg caaaaaagaa accgaagcgg tggccggcaa
     9181 gtacggcgtc accctggtgg ccagtgnnnn nnnnnngccc aaggacaccg acatgagccc
     9241 gcagctgacc aagatcaaga acacccccgg cgtgcaggcg ctgttcatct ttggcctggg
     9301 ccagggcccg gccatcgcca ccaagaacta caagcaactg gggctgaccc tgcccttgta
     9361 ccacgcccac ggcgtggcct cggaagagtt catcaagctg gccggcccgg cggccgaagg
     9421 cgtgcgactg ccggcgacgg ctttgctggt gatcgacaaa ctggacgcca aagacccgca
     9481 aaaaccggtg gtcaccaact acgcccgtgc gtttcaggcg cgctggaaaa ccgatgtgtc
     9541 ttcgtttggc ggttatgcct acgacgggct gatgctgacg gtggatgcca tcaagcgcgc
     9601 tggcagcgcc gacaaggcca aggtgcgcga cgcgctggaa gccaccaagg gttatgtcgg
     9661 cgccagcggc aaggtgaata tgagcgccag cgaccatatg ggcctggatt tgtcggcctt
     9721 ccgcctggtg gaaatcaaag ccggcgactg ggcgttgttg ggcagcaagt gaaagggccg
     9781 tcatgactga ttctgtgctc accgaaaccc acggccgggt ggggctgatc cgctttaacc
     9841 gccccgaggc gatgaatgcg ctgaacgccg acgtggtggc ggcgctggcc gccgccatcg
     9901 acgactttga agccaacccg gacatcggct gcctggtgct caccggcagc gacacggtgt
     9961 ttgccgccgg cgccgacatc ggcttcatga aggatttcag ctatctgcac gcctaccgga
    10021 cagactttgt caccaccggc ggctgggagc ggctgaaaac cacccgcaag ccggtgattg
    10081 ccgccgtggc gggctttgcc ctgggcggcg gctgcgagct ggcgatgatg tgcgacatga
    10141 tttttgc
//
LOCUS       DAOPYX010000084        10121 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_7774_length_10121_cov_8.049970, whole genome shotgun sequence.
ACCESSION   DAOPYX010000084 DAOPYX010000000
VERSION     DAOPYX010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 10121)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10121)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10121
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7774_length_10121_cov_8.049970"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..803)
                     /locus_tag="PKN55_05930"
     CDS             complement(<1..803)
                     /locus_tag="PKN55_05930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014086883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HNX70812.1"
                     /translation="MKKSLIPLLLALLATPALADKLPVLASFSILADLTREVGGERVD
                     VASLVGPDQDAHVFQPAPQDVKKLAAAKVFVVNGLGFEGWLSRLSKSSGFKGQTVVAS
                     QGVKAQDMAEEEHDHHGHDHGHTDPHAWHNPQNVKLYVNNIRAALTQADPAGKDYYSQ
                     RASQYSQQLDALDADARQRFAAIPASQRKVLTSHDAFGYLAQRYQIRFLSPQGISTDA
                     EASAKTVAKLVRQVKAEKIRAVFVENMSDKRLLEQLSREAGVQVGGKLYA"
     gene            complement(833..1708)
                     /locus_tag="PKN55_05935"
     CDS             complement(833..1708)
                     /locus_tag="PKN55_05935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011136612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HNX70813.1"
                     /translation="MNELYTLLVQPFIEFGFMRRGLVGCLALALGCGPIGLFLVMRRM
                     SLMGDALSHAVLPGAAIGFIFAGLSLPAMGLGGFVAGISVALIAGLVSRFTSVREDAS
                     FAAFYLLALSVGVLLVSKYGSNVDLIHILFGTVLAVDDAALLLVASIASLTLLTLAVI
                     YRPLLLESYDPAFLQSVGASGGAYHLIFLVLVVLNLVAGFQALGTLMAVGLMMLPAIT
                     ARLWAQSVGGMLLTAITLALSSGLGGLLCSYHFDLPSGPAIILFAGSLYLLSLLFAPA
                     GGVLPRLIRQRHLAH"
     gene            complement(1705..2463)
                     /locus_tag="PKN55_05940"
     CDS             complement(1705..2463)
                     /locus_tag="PKN55_05940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018152856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNX70814.1"
                     /translation="MSVRLSNLTVSYRRHPAVHHVSGEFAAGQATAIVGPNGAGKSTI
                     LKTMIGALRPDAGRVELGGVSRQHIAYLPQAAQIDRSLPVSVLDTVLTGAWRHMGAFG
                     AARAQDWQAAQDALAAVGLGEFAQRPVSALSAGQFQRVLFARIMVQDAPLILLDEPFT
                     AIDARTTHDLLALVRRWQAEQRTVIAVLHDFAQVREYFPHTLLLSREVVAWGPTAEVL
                     TEANLQRASANVAHWHEQAPVCRVDDAAAQEAQP"
     gene            complement(2460..2594)
                     /locus_tag="PKN55_05945"
     CDS             complement(2460..2594)
                     /locus_tag="PKN55_05945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX70815.1"
                     /translation="MKANRLSRRFSLLAASAGQRLAMALLAIAGLWTAVIWALSEGGL
                     "
     gene            complement(2820..3752)
                     /locus_tag="PKN55_05950"
     CDS             complement(2820..3752)
                     /locus_tag="PKN55_05950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008953901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HNX70816.1"
                     /translation="MSKTPINLFTGFLGAGKTTALLHLLAHKPADEVWAVIVNEFGEI
                     GIDGAVLSGGGAEVREIAGGCLCCATGPQLPVTLVKLIREKRPHRVLIEASGLAHASN
                     VVDELRRPPLGEALDIQASITVVDPRQFIDANYRRHPIYRDQIELADVLAASKADLAD
                     AATLAAFQEQAQALFPPKARIEVMQHGQLDPAWLSLPARPAARYRPKLAPADSGMSSA
                     GWTFASEVVFDLERVGALLRTLPQRVPGLARAKAVLRMGPHDWLWFNWVAGGEWAATQ
                     VAWRRDSRFELIAPPGDWAAEIEAELGRAIAAVA"
     gene            complement(3749..4198)
                     /locus_tag="PKN55_05955"
     CDS             complement(3749..4198)
                     /locus_tag="PKN55_05955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008953900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HNX70817.1"
                     /translation="MTPLSPEEFLLIAQQHCAARGARLTELRQQVLGLVLARGGVVKA
                     YQVLTDLQAARGPAAPPTVYRALDFLVEQGLLHRVDALNGYIACQHLECQHHGLLLVC
                     ETCNDVTEIDATDSLDQLSAAAARAGFQPRRQDVLLTGLCQSCRQPS"
     gene            4395..4841
                     /locus_tag="PKN55_05960"
     CDS             4395..4841
                     /locus_tag="PKN55_05960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012696818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HNX70818.1"
                     /translation="MRLLSTLLAAVLATTAFSAVAAEAKDPVEARKAIFKDYKKVFGQ
                     GMGAVMKGEKPYNKDEFAKLAEQMVELSKQPWQYFPAGSDKGKSEARPEVWSKPAEFK
                     KAADEHQALIVKLAEAAKGGDLAQIRPAFAAAQKTCKACHEVFKKD"
     gene            4996..5742
                     /locus_tag="PKN55_05965"
     CDS             4996..5742
                     /locus_tag="PKN55_05965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX70819.1"
                     /translation="MTLSAVQAQTRATNISGASPADRQAAGFAAALAKYARQLDEQTH
                     SLASQIQPAARPAPPTPATLRSDGATEIYDVGAGPMALDIDAYFTPPRPGEQVDLNTL
                     PLLMPTENNLRALSQHIAKRLPDALKAYGIPSAPAYVRYDSSGQLVLPDNYPYADKFK
                     AMLNEQPALSRQLSTLNGLTSHLVELRKVEPFQREYAAANTQAEIDAVLKKYSYLFSG
                     QRTYSMITITLSGNGAAVSLSADDKPVFSA"
     gene            complement(6079..8382)
                     /locus_tag="PKN55_05970"
     CDS             complement(6079..8382)
                     /locus_tag="PKN55_05970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008953874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HNX70820.1"
                     /translation="MLPSIASRLAAELAVRESQVAATVQLLDDGATVPFIARYRKEAT
                     GGLDDTQLRQLDERLRYLRELDDRRATILASIGEQGKLSAELEAALYAATDKTTLEDL
                     YLPYKPKRRTKAQIAREAGLEPLADSLLTDPTQDPQHVALAYVNAEAGVADAKAALDG
                     ARAILIERMGEHAALLGELREKLWREGEMRSTVVEGKAEAGAKFADYFAYAEPIRAIP
                     SHRALALLRGRNEGVLNVQLSWQPADAPTTQRSDYELMIAEAFDIADRGRPADKWLLD
                     GVRLAWRAKIVLSLELELTQRLREAAEGEAIQVFARNLHDLLLAAPAGPRPTLGLDPG
                     LRTGVKVAVVDATGKLVDAGAIYPHAPRNDWDKSIAVLAALCARHRVELIAIGNGTAS
                     RETDKLAADLMRLHPELKLTKLVVSEAGASVYSASELAAREFPELDVSLRGAVSIARR
                     LQDPLAELVKIDPKSIGVGQYQHDVNQSQLARSLDAVVEDCVNAVGVDVNTASAALLA
                     RVSGLNSSIAGNIVSYRDSHGAFANRKTLLKVPRLGDKTFEQAAGFLRIMNGDNPLDR
                     SAVHPEAYELVERIIARCNKPVTALIGDASALKTLKPSDYTDERFGLPTVMDILRELE
                     KPGRDPRPEFKTAAFADGVEDLADLRPGMILEGVVTNVANFGAFIDIGVHQDGLVHIS
                     ALSKQFVDDPRKVVKAGDVVKVKVMEVDVKRKRIGLSMRLDDEPGASTARGPRDDAPA
                     RKGGGKPREAAPPANNAMAAALAKLRR"
     gene            complement(8555..8902)
                     /locus_tag="PKN55_05975"
     CDS             complement(8555..8902)
                     /locus_tag="PKN55_05975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009116917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS/SEC14 domain-containing protein"
                     /protein_id="HNX70821.1"
                     /translation="MISIQQQSYGLDVALFATFTVDDFRAFETAVLARAQTDGRVNVL
                     LNLSGLLDFTIDMALEELRFVREHEQEFGRIAIVAPEGWLNLAAHIAGLLSHTDSEYF
                     DTPEDALAWLNAN"
     gene            complement(8955..9824)
                     /locus_tag="PKN55_05980"
     CDS             complement(8955..9824)
                     /locus_tag="PKN55_05980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008953871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNX70822.1"
                     /translation="MTFSQALTRLKTGQLGAGWMVVASLLFALMGVFVKTGSAHFSAI
                     ELVFYRTLFGMLSIGVVVLARGQSLATRHFSAHLKRGLFGYFSLLLYFYAITQLPLAT
                     AVTLNYTSPMFLAGLSAWLLKETLGPRVVLSLGLGFAGVVLLLKPTFSADAWLAGAMG
                     LGSGLFAGLAYLHVRELGRLGEPEWKVVFYFTLISTLCGLVMMLWQTPSPLGMESLGM
                     LAGLGVTSTLAQLAMTRAYRDGNKFVVANLAYLTVVFSSALGAWLWGDVLPLEGYIAM
                     GLIIVSGIFASRR"
BASE COUNT         1871 a         3275 c         3260 g         1715 t
ORIGIN      
        1 gcatagagct tgccgcccac ttgcacgccg gcttcgcggc tgagttgctc cagcaggcgc
       61 ttgtcgctca tgttttcgac aaacaccgcg cggatttttt cggctttcac ctggcgcacc
      121 agcttggcca cggttttagc cgaggcttcg gcgtcggtgg agatgccttg cggggacaaa
      181 aagcggattt gatagcgctg cgccaggtag ccaaaggcgt cgtggctggt cagcaccttg
      241 cgctggctgg ccgggatggc ggcaaagcgc tggcgggcgt cggcgtccag tgcgtccagc
      301 tgctggctgt actggctggc gcgctggctg tagtagtcct tgccggccgg gtcggcctgg
      361 gtgagcgcgg cgcggatatt gttgacgtag agtttgacgt tttgcgggtt gtgccaggcg
      421 tgcgggtcgg tgtggccgtg gtcgtggccg tgatgatcgt gttcttcttc ggccatgtct
      481 tgcgccttca cgccctggct ggccaccacg gtctggccct tgaagccgga agatttgctc
      541 agccggctca gccagccttc aaagcccagg ccgttgacca caaacacctt ggcggcggcc
      601 agttttttga catcctgcgg cgccggctga aacacatggg cgtcttgatc ggggccaacc
      661 agactggcca cgtccacccg ctcgccgccc acttcgcggg tgaggtcggc cagaatgctg
      721 aagctggcga gcaccggcag tttgtcggcc agcgccggcg tggccagcag cgccagcagc
      781 agggggatca gggatttttt catgcttgcg tccttgggtg acaacggggg aatcagtgcg
      841 ccagatggcg ctggcggatc aaacgcggca acaccccgcc agccggggca aacagcagcg
      901 acaataaata caggctgccg gcaaacaaaa taatcgccgg gccagaaggc aggtcaaagt
      961 gatacgagca cagcaggccg cccaaacccg agctcagcgc cagggtgatg gcggtcagca
     1021 gcatgccgcc cacgctttgc gcccacagcc gggcggtgat ggccggcagc atcatcaagc
     1081 ccaccgccat caaggtgccc agcgcctgaa aaccagccac cagattgagc accaccagca
     1141 ccagaaaaat caggtgatac gccccgcccg aagcgccaac cgactgcaaa aacgccgggt
     1201 cgtagctttc cagcagcagc ggccggtaga tcaccgccag ggtgagcagg gtgagcgagg
     1261 caatcgaggc caccagcagc agcgcggcgt cgtccaccgc cagcacggtg ccaaacagga
     1321 tatggatcag atcgacattg ctgccgtact tggacaccag cagcacgccc accgacagcg
     1381 ccagcaggta aaacgcggca aagctggcgt cctcgcgcac gctggtgaag cggctgacca
     1441 ggccggcaat cagcgccacg gaaatcccgg cgacaaagcc gcccagcccc atggccggca
     1501 gcgacagccc ggcaaaaata aagccgatgg ccgcgcccgg cagcacggcg tggctgagcg
     1561 cgtcgcccat caggctcatg cgccgcatca ccaaaaacag cccaatcggc ccgcagccca
     1621 gcgccagcgc caggcagccg accaggccac ggcgcataaa gccgaattca ataaagggct
     1681 gcaccagcag ggtgtaaagc tcgttcatgg ctgggcctcc tgcgcagcgg cgtcatccac
     1741 ccggcacacc ggggcttgtt catgccagtg ggcgacattg gcgctggcgc gctgcaaatt
     1801 ggcttcggtc agcacctcgg cggtcggccc ccaggccacc acctcgcgcg acagcagcaa
     1861 ggtgtgcgga aaatactcgc gcacctgggc aaaatcatgc agcacggcaa tcaccgtgcg
     1921 ctgctcggcc tgccagcggc gcaccagcgc cagcaaatca tgcgtggtgc gcgcgtcgat
     1981 ggcggtgaat ggctcgtcca gcaaaatcag cggcgcgtcc tgcaccatga tgcgggcgaa
     2041 caatacccgc tgaaactgcc cggccgacaa ggcgctgacc gggcgctggg caaactcgcc
     2101 caagcccacc gccgccaagg catcctgcgc cgcctgccaa tcctgcgccc gcgccgcgcc
     2161 aaacgcgccc atatgccgcc aggcgccggt cagcacggtg tccagcacgc tcaccggcaa
     2221 gctgcggtcg atctgcgccg cctgcggcag ataggcgata tgctgccggc tcaccccgcc
     2281 cagctccacc cgcccggcat ccgggcgcaa ggcgccgatc atggttttca ggatggtgct
     2341 cttgcccgcg ccattcggcc cgacaatcgc cgtggcctgg ccggcggcaa actcgccgct
     2401 gacatggtgc acggcggggt ggcgacggta agacaccgtc aaattagaca aacgcacgct
     2461 cacagcccgc cctcggacaa ggcccagatg accgccgtcc acaagccggc gatggccagc
     2521 aaggccatgg ccagacgctg accggcagaa gcggccagca ggctgaagcg acgggataac
     2581 cggtttgctt tcatgggtat tcctgaaatg acgccgcccc agggtgaagt ggggcggtgt
     2641 ggttgttcta tcgttgtgac gccgggtgct gcgacttacc cttgtggcgc ggtgtgcatg
     2701 ctcgccaaac gcggccaaag caggcttgcc ctcagtgcgt cgtggagact tgcctgggtc
     2761 cccgcctgcg cggggacgac gcggaagtgg gtgacgccac tgtggtggcc agtgtggcgt
     2821 cacgccacgg cggcaatcgc ccggcccagt tcggcttcaa tttcagccgc ccagtcgcct
     2881 ggcggggcga tgagttcaaa gcggctgtcg cgtcgccagg ccacttgggt ggcggcccat
     2941 tcgccgccgg ccacccagtt gaaccataac caatcgtgcg ggcccatgcg cagcaccgcc
     3001 ttggcgcgcg ccaggccggg cacgcgctgc ggcagggtgc gcaaaagcgc gcccacccgc
     3061 tccaggtcga acaccacttc gctggcgaag gtccagccgg cgctgctcat gccgctgtcg
     3121 gccggggcca gtttcgggcg gtagcgggcg gcagggcggg ccggcaggct gagccaggcg
     3181 gggtcgagct ggccgtgctg catcacttcg atgcgcgcct tgggcggaaa cagcgcttgg
     3241 gcttgttcct gaaacgccgc cagggtggcg gcgtcggcca ggtcggcctt gctggcggcc
     3301 agcacgtcgg ccagttcgat ctggtcgcgg tagatcgggt ggcggcggta attggcgtcg
     3361 ataaactgac gcggatccac cacggtgatg gaggcctgaa tgtccagcgc ctcgcccagc
     3421 ggcgggcggc gcagttcgtc caccacattg gaggcgtgcg ccaggccgct ggcttcgatc
     3481 agcacgcggt gcgggcgttt ttcccgaatc agcttgacca gggtgaccgg cagttgcggg
     3541 ccggtggcgc agcacaggca gccgccggcg atttcgcgca cttcggcgcc gccgccagac
     3601 agcacggcgc cgtcgatgcc gatttcgcca aattcgttga cgatcaccgc ccagacttca
     3661 tcggccggct tatgcgccag cagatgcagc aaggccgtgg ttttgcccgc gcccagaaag
     3721 ccggtgaaca gattgatggg ggttttgctc atgagggttg tctacagctt tggcaaagcc
     3781 cggtcagcag cacatcctgg cggcgcggtt gaaaaccggc gcgcgcggcg gcggcgctga
     3841 gttgatccag gctgtcggtg gcgtcgattt cggtgacgtc gttacaggtt tcgcacacca
     3901 gcagcaggcc gtggtgctgg cattccaggt gctggcaggc aatatagcca ttcagcgcgt
     3961 ccacccggtg caacagccct tgctcgacca gaaaatccag cgcgcggtat acggtgggcg
     4021 gcgcggccgg accgcgggcg gcttgcaggt cggtcagcac ctggtaggct ttgaccacgc
     4081 cgccgcgcgc cagcaccagg cccagcactt gctggcgcag ctcggtcagc cgcgcgccac
     4141 gggcagcgca gtgctgctgg gcgatcagca ggaactcttc gggggatagc ggcgtcacag
     4201 ccttcgctct cacttggaat aatcgacgat ttgttacagt ataacatcac atcggccagg
     4261 ctgaccagcc gctggcgcca gtgattgcgt gcggttatca ttatttaaaa atatgaatta
     4321 ttgccagcca cggcgcttac cccgtatgct ggcgtggatt tcccccaaca tcatcattca
     4381 aaaggatgag tttcatgcgt ctgctttcta ctctgctggc cgccgtgctg gccaccactg
     4441 cgttttctgc cgtcgccgcc gaggccaagg atccggttga agcccgcaag gcgattttca
     4501 aggactacaa aaaagtcttc ggccagggca tgggcgcggt gatgaagggc gaaaagccgt
     4561 ataacaagga cgagttcgcc aagctggccg agcagatggt cgagctgtcc aagcagccgt
     4621 ggcaatactt cccggccggc agcgacaagg gcaagagcga agcccgcccg gaagtgtgga
     4681 gcaagccggc tgagttcaag aaagccgccg atgagcatca ggcgctgatc gtcaagctgg
     4741 ccgaagccgc caagggtggc gatctggcgc aaatccgccc ggcgtttgcc gctgcgcaaa
     4801 aaacctgtaa ggcttgccac gaagtgttca agaaagactg attcaccctg cacgccccat
     4861 gcaaaacgcc cgccttttgg tgggcgtttt gcatggcaga ggctggcgcg ctttttgccc
     4921 tatggcagtg ggcccacgct gggccgcaca tgcaatcagc catgtgcact gagttgtttt
     4981 taaaggataa aaacgatgac gctttctgcc gtgcaggcgc aaacccgcgc caccaacatc
     5041 tctggcgctt cgcctgccga ccgccaggcg gcaggctttg ccgccgccct ggcaaaatat
     5101 gccaggcaac tcgacgagca aacgcacagc ctggccagtc aaattcaacc cgctgcccgc
     5161 ccggcgccgc ccaccccggc taccttgcgc agcgatggcg ccactgagat ctacgatgtc
     5221 ggcgccggcc cgatggcgct ggatatcgac gcgtatttca ccccaccgcg tccaggcgag
     5281 caggtggatt tgaacaccct gccgttgttg atgcccacgg aaaacaatct gcgcgccttg
     5341 tcccagcaca ttgccaagcg cctgcctgat gcgctgaaag cctacggcat ccccagcgca
     5401 ccggcctatg tgcgttacga cagcagtggc caattggtat tgccggacaa ttacccgtat
     5461 gccgacaagt tcaaggccat gctcaacgaa cagccggcgc tgtcgcggca gttaagcacg
     5521 ctgaacgggc tgacttcaca tttggtggag ctgcgcaaag tcgagccctt ccagcgcgaa
     5581 tacgccgcgg ccaacaccca ggcggaaatc gacgccgtgc tgaaaaagta cagctatttg
     5641 ttctccgggc agcgcactta ctccatgatt accatcaccc tgagcggcaa tggcgctgcg
     5701 gtgagcctga gcgcggacga caagcccgtg ttcagcgcgt agcgccactc acaaaaccct
     5761 gggcgttgtg gtgtggcctt gccgtgtctg tggccatacg cctggtcaga gccgtttcgc
     5821 tgagtgttgt tagccgcgct acccaccaaa ccctgctggc gtgcttgact cctgtgcggt
     5881 gtgatgcaag cagcgcttta gcgtcgctca caacacccag cgcagcgcgc ctggcgaggc
     5941 gagcgaccgc agacagtaca agcagtacgg caaggtcgga acgacgccag gaggggcttg
     6001 ttagcgactc ttactgtgtt gccatgcccg gcggtttgcc tttgcttacg ccgggcaggc
     6061 tgtggcggta tgccgcgctc agcgccgcag cttggccagc gccgccgcca tggcgttatt
     6121 ggccggcgga gccgcctcgc gcggcttgcc gccgcccttg cgcgccgggg cgtcgtcacg
     6181 cgggccgcgc gccgtgctgg cgcccggctc gtcgtccagg cgcatgctca ggccaatgcg
     6241 cttgcgcttg acgtccactt ccatcacttt caccttgacc acatcgccgg ccttgaccac
     6301 cttgcgcggg tcatcgacaa actgcttgct cagggctgaa atatgcacca ggccgtcctg
     6361 atgcacgccg atgtcgataa aagcgccaaa gttggcgaca ttggtcacca cgccttccag
     6421 aatcatgccg gggcgcaggt cggccaggtc ttccacgccg tcggcaaagg cggcggtctt
     6481 gaattccggg cgcgggtcgc ggccgggttt ttccagctcg cgcaggatgt ccatcaccgt
     6541 gggcaggcca aagcgctcgt cggtgtagtc gctgggcttg agggttttca gcgcgctggc
     6601 gtcgccaatc agcgcggtga ctggcttgtt gcagcgggca atgatgcgct cgaccagttc
     6661 gtaggcttcc gggtgcacgg cggagcggtc cagcgggttg tcgccgttca tgatgcgcaa
     6721 aaagccagcg gcctgttcaa aggttttgtc gcccaggcgc ggcactttca gcagggtttt
     6781 gcggttggcg aaggcgccgt ggctgtcgcg gtagctgacg atattgccgg caatgctgct
     6841 gttcagcccg gacacccgcg ccagcagcgc cgccgaggcg gtgttgacgt ccacgcccac
     6901 cgcgttcacg cagtcttcca ccaccgcgtc cagcgagcgc gccagctggc tttggttgac
     6961 gtcgtgctga tactggccca cgccgatgga tttcgggtcg atcttcacca gctcggccag
     7021 cgggtcttgc aggcggcggg caatcgacac cgcgccgcgc agcgacacat ccagctcggg
     7081 gaactcacgc gcggccagtt ccgaggcgga atacaccgag gcgccggctt cggagaccac
     7141 cagcttggtc agtttcagtt ccgggtgcag gcgcatcagg tcggcggcca gcttgtcggt
     7201 ttcccggctg gcggtgccgt tgccgatggc gatcagctca acgcggtggc gggcgcacag
     7261 cgcggccagc acggcgatgg atttgtccca gtcgttgcgc ggcgcgtgcg ggtagatggc
     7321 gccggcgtcc accagcttgc cggtggcgtc caccaccgcc accttgacgc cggtgcgcag
     7381 gccggggtcc agacccaggg tggggcgcgg gccggccggc gcggcgagca aaaggtcgtg
     7441 cagattgcgg gcaaacacct gaatcgcctc gccttcggcc gcctcgcgca ggcgctgggt
     7501 gagctccagc tccagcgaca gcacaatctt ggcgcgccag gccagccgca cgccgtccag
     7561 cagccatttg tcggccgggc ggccacggtc ggcgatgtcg aaggcctcgg caatcatcag
     7621 ctcgtagtcg ctgcgctggg tggtcggcgc atcggccggc tgccagctca gctgcacatt
     7681 cagcacgcct tcgttgcggc cgcgcagcag cgccagcgcg cggtgcgagg gaatcgcccg
     7741 aatcggctcg gcgtaggcaa aataatcggc aaacttggcg ccggcttcgg ccttgccttc
     7801 caccacggtc gagcgcattt cgccctcgcg ccagagtttt tcgcgcaact cgcccagcag
     7861 cgcggcgtgc tcgcccatgc gttcgatcag gatggcgcgt gcgccgtcca gcgccgcctt
     7921 ggcgtcggcc acgccagcct cggcgttgac ataggccagc gcgacgtgtt gcggatcttg
     7981 tgtcgggtcg gtcagcaggc tgtcggccag cggctccagc ccggcttcgc gggcaatctg
     8041 cgccttggtg cgccgcttgg gcttgtaggg caggtaaagg tcttccagcg tggtcttgtc
     8101 agtggcggcg tacagcgcgg cttcgagttc ggcgctgagc ttgccctgtt cgccaatgct
     8161 ggccaggatg gtggcgcggc ggtcgtccag ctcgcgcagg tagcgcaggc gctcgtccaa
     8221 ctggcgcagc tgggtgtcgt ccaggccgcc agtggcttct ttacggtagc gggcaatgaa
     8281 ggggacggtg gcgccgtcgt ccagcaactg gacagtggcc gccacctggg attcacgaac
     8341 cgccagttcg gcggccaggc gggaagcaat cgaaggcaac atcgggggct atctggtcag
     8401 gcaaaggcgt gactttaacg gattttccgg gtgcaggtaa gtgttgccgc catgcccacg
     8461 ctcacctgac ccagcgccgc gcccccggcc cggcgtgtga gcgcagagcg ttgcgcagca
     8521 caccatcagg ccagagaagg atgataagcg gcgcttagtt tgcgttcagc caggccagcg
     8581 catcctccgg ggtgtcgaaa tattcgctgt cagtgtggct gagcaggccg gcaatatgcg
     8641 cagccagatt caaccagccc tcgggggcga caatggcgat gcggccaaat tcttgctcgt
     8701 gctcacgcac aaagcgcaat tcttccagcg ccatgtcaat ggtgaaatcc agcaggccgc
     8761 tcaggttgag cagcacattc acccggccat cggtttgcgc gcgcgccagc acggcggttt
     8821 caaaggcgcg aaagtcatct acggtaaagg tggcgaacag cgccacatcc aggccgtaag
     8881 actgttgttg gatggaaatc atggtcaggc tcctgaaaac gccggcgctc agacgcgccg
     8941 gtcatgcttt cagcttagcg cctgctggca aaaatgccgc tgacgataat caaacccatg
     9001 gcgatatagc cttccagcgg cagcacatcg ccccacagcc aggcgcccag cgcgctggaa
     9061 aacaccacgg tgaggtaggc cagattggcc accacaaatt tattgccatc gcggtaggcg
     9121 cgggtcatcg ccagttgtgc cagcgtggaa gtgacaccca ggcctgccag catgcccaga
     9181 ctttccatcc ccagcgggct gggcgtttgc cacagcatca tgaccaatcc gcacagcgtg
     9241 gaaatcagcg tgaaatagaa caccactttc cattccggct cacccagccg gcccagctcg
     9301 cgcacatgca gataggccaa cccggcaaac agcccggaac ccaggcccat ggccccggcc
     9361 agccaggcgt cggcgctgaa ggtgggcttg agcagcagca ccacgccggc aaagcccagg
     9421 cccagcgaca gcaccacccg tgggccgagt gtttctttca gcagccaggc agacaacccc
     9481 gccagaaaca tcggcgaggt gtagttcagc gtcaccgccg tggccagcgg cagctgggtg
     9541 atggcgtaaa agtacaagag cagcgagaaa tagccgaaca gcccgcgctt gaggtgggca
     9601 gaaaaatggc gggtggccag cgactgcccg cgcgccaaca ccaccacgcc aatgctgagc
     9661 atgccaaaca gcgtgcggta gaacaccagc tcgatggcgg aaaaatgcgc cgagccggtt
     9721 ttgacgaaca cccccatcag ggcaaacagc aaagacgcca ccaccatcca gccagccccc
     9781 agctggccgg ttttcaggcg ggtgagggct tgagagaagg tcatgcggga tttcctgtga
     9841 ttctggcgtg gccaccggct gatgcggcgg cgcaacaaag cgtcacctta accgtgatgc
     9901 ctggcgctgg caaccttggg tcatctttgg cttgagtgta atatagtccc ctaccctata
     9961 tttagcgttg ctggcccaat cctgcgcagc gttctgggcg gcagactgca ccccaagcag
    10021 aaaaggattg ggtgagcgac gctcatcacc gccagcccca ggagggcttc atgtctcaca
    10081 cccaacagga tcaagccaaa ttactggcgc gcgtgcgtcg g
//
LOCUS       DAOPYX010000085        10101 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_7791_length_10101_cov_12.135975, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000085 DAOPYX010000000
VERSION     DAOPYX010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 10101)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10101)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10101
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7791_length_10101_cov_12.135975"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..932)
                     /gene="draG"
                     /locus_tag="PKN55_05985"
     CDS             complement(<1..932)
                     /gene="draG"
                     /locus_tag="PKN55_05985"
                     /EC_number="3.2.2.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR02662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047407 - ADP-ribosyl-[dinitrogen
                     reductase] hydrolase activity [Evidence IEA];
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosyl-[dinitrogen reductase] hydrolase"
                     /protein_id="HNX70823.1"
                     /translation="MLWPGVLNRIRQFGPAPSAPLTLEARALGAYLGLAVGDALGATV
                     EFLTPNEIRHQIGLHRHLTGGGWLKLRPGMVTDDTTMSLALGEAILLSGGRVDALLAA
                     QAFDQWMRGKPVDIGNTVRRNLMRFRRTGEPCAPPSEHDAGNGAAMRLLPAALATFGL
                     DADTVREAVLAQAHVTHHSPLSDAACLTLTGMLHALLAGADKKTVLQQHVQPLIQQYP
                     VFGFRARRCDNPSGYIVDTLQAVFQAFFDTDTLEDCLIDVVNRGGDADTTGAIAGMLA
                     GAHQGPKAIPRRWLSVLDADTRAACELQSWLLLPA"
     gene            complement(911..1489)
                     /locus_tag="PKN55_05990"
     CDS             complement(911..1489)
                     /locus_tag="PKN55_05990"
                     /inference="COORDINATES: protein motif:HMM:NF016762.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen fixation protein NifQ"
                     /protein_id="HNX70824.1"
                     /translation="MIPPDRLHHRALLCASLLQGDAGPAPVDEGNRSLLASLLAGQRA
                     GLGCLPPQLGLADNDFAAMLAAYFPTARLPAPWPAASPERVPEWDELVQFLLDHRAGE
                     HAEPRWMAQILAAACAGRDHLWQDLGLTGRHELSQLIALNFPALAQANDGDMKWKKFI
                     YRQLCAREGIYVCPAPSCQACHDRPACFGPES"
     gene            complement(1588..1950)
                     /locus_tag="PKN55_05995"
     CDS             complement(1588..1950)
                     /locus_tag="PKN55_05995"
                     /inference="COORDINATES: protein motif:HMM:NF012339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HNX70825.1"
                     /translation="MALITFSSRLHKDKTVYAVAGSHTQTILTLAKENHIPIDFGCGN
                     GECGTCLVKVSSVDTKRSPMGGPLTNREAVVLKDMGYVTQPELDQMYVDDIPPTQWRL
                     ACQMVVRDEDILVEYPSK"
     gene            complement(2076..2363)
                     /locus_tag="PKN55_06000"
     CDS             complement(2076..2363)
                     /locus_tag="PKN55_06000"
                     /inference="COORDINATES: protein motif:HMM:NF012339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HNX70826.1"
                     /translation="MPKAKVIFEDIGVAVTVPAGTRLIEVSEKVGAGITYGCREGECC
                     TCLTKVVSGGDNLAAPSVLEDQVLKDNMAPRNHRLACQAQILGGEVVVRPA"
     gene            complement(2388..3665)
                     /locus_tag="PKN55_06005"
     CDS             complement(2388..3665)
                     /locus_tag="PKN55_06005"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HNX70827.1"
                     /translation="MSIVHPDQAVELAPGVHWIGALDPHLRRFDIILSTANGTSYNAY
                     LVRGEQGVAVIDTVKEAFADDFFRRLESVARYDEITTIVLNHLEPDHSGALPELLRRA
                     PNARVYLSSRAQMMLKALLKPSSNQAPPDYTPVTTGDTIDLGGRTLAFFHTPYLHWPD
                     TQCTWLVEEGMLFSGDVFGCHYCDPRLFNDRVGDFRFSFEYYYAHIMRPFRAHVLDAL
                     ELLEPLDIRLIAPAHGPILRHHPRDYLARYREMATPRLALDTEAAGEKTLAVFYLSAY
                     GNTRSMAEAIAIGAERIAGVRVSLFDLEGGDTAHFTDLIEAADGLAFGSPTLNGDAAP
                     PIWALLSSLTTVNVKGKFGAAFGSYGWSGEAVRLIEDRLRGLKLRVPVEGLRLKLAPD
                     ASELDACRTLGETLARHLVGEAMPREVDLASLS"
     gene            complement(3646..4113)
                     /locus_tag="PKN55_06010"
     CDS             complement(3646..4113)
                     /locus_tag="PKN55_06010"
                     /inference="COORDINATES: protein motif:HMM:TIGR01616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC/Spx/MgsR family protein"
                     /protein_id="HNX70828.1"
                     /translation="MATLTFYEKPGCQGNARQKALLRAAGHQLHVRNLLTEPWTPERL
                     RQFFGALPVAQWFNRNAPAVKSGEVNPDALDADAALALMVKRAILIRRPLLEMGDERW
                     VGFDVAALDARIGLNGVNLPPGDLEACSHHGAEAAAHAAACHAEGHAHEHCSP"
     gene            complement(4113..4394)
                     /locus_tag="PKN55_06015"
     CDS             complement(4113..4394)
                     /locus_tag="PKN55_06015"
                     /inference="COORDINATES: protein motif:HMM:NF012267.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNX70829.1"
                     /translation="MALQIVDLCVNCWACVDVCPNQAVVEDVPHFLINEQACTECLGD
                     HAEAQCAAICPIEGAIVNELGEPLNPPGSLTGIPLDKLRELGLLDREAA"
     gene            complement(4395..5903)
                     /gene="nifB"
                     /locus_tag="PKN55_06020"
     CDS             complement(4395..5903)
                     /gene="nifB"
                     /locus_tag="PKN55_06020"
                     /inference="COORDINATES: protein motif:HMM:TIGR01290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogenase cofactor biosynthesis protein NifB"
                     /protein_id="HNX70830.1"
                     /translation="MELPVISNAPSSGGGCSSSGCGSSSDAMAHLPDSIRQKVQDHPC
                     YSEDAHHYFARMHVAVAPACNIQCNYCNRKYDCANESRPGVVSELLHPDQAVTKVLAV
                     AATIPQMTVLGIAGPGDPLANPARTFETFRQLSDKAPDIKLCLSTNGLTLPEHVDEIA
                     KHNIDHVTITINCVDPEIGAQIYPWIYWQNRRLRGVEAARILIEQQQKGLEMLTSRGI
                     LVKVNSVMIPGVNDEHLKEVSKVVKAKGAFLHNVMPLIAEAEHGTYYGLMEQPSPTPE
                     QLQALQDECGGDMAMMRHCRQCRADAVGLLGEDRGAEFTLDKLADMDIDYEAAMQTRQ
                     EVRENLLEQLAAKRAGAPAAEVTALAGVPADSRPVLMAVAAKNGVVGEHFGHAREFLV
                     YEASPKGVRLMGHRKVDLYCNGATDCGDSGEGGQPAVSILDQIIHALSGCEQVLCARI
                     GFEPWGQLEAAGIQPNGEHAMEPVEDAVLAVYREMSAAGKLAAPADKLNKVA"
     gene            complement(5989..6717)
                     /locus_tag="PKN55_06025"
     CDS             complement(5989..6717)
                     /locus_tag="PKN55_06025"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HNX70831.1"
                     /translation="MTVFLRTLFFVTLGLAAWQAHADVYGFVDEQGEVHLADKPLDAR
                     YRLFKRGALPAASSSAATAELGRYLDARDQAALKRLTPLIEQTARRHQLEPALLHAVI
                     TVESGYHASAASPKGAQGLMQLMPDTAARFGVSAETVLEPRANLDAGARYLAFLLGLF
                     DRRLDLALAAYNAGEGVVQRLGRIPPYEETEQYVKAVRGLYLRQGGRVLADGRLRGHA
                     VADTQTYANLVRFTTSDATRTEDQ"
     gene            6865..7278
                     /locus_tag="PKN55_06030"
     CDS             6865..7278
                     /locus_tag="PKN55_06030"
                     /inference="COORDINATES: protein motif:HMM:NF039485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HNX70832.1"
                     /translation="MGLLDQLSDAISGGVQQLQDTVGQAGGALGDLLQNSGGIDGLVE
                     RFQQAGLGDTLQSWISQGENLPISAEQLQQVFSADTLNSLAEKAGIDPAQAGEWLSAN
                     LPEWVNQLTPNGSLSDLGGVFEQGVSAVKDFFSKF"
     gene            7438..8712
                     /locus_tag="PKN55_06035"
     CDS             7438..8712
                     /locus_tag="PKN55_06035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012698413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmpG family muropeptide MFS transporter"
                     /protein_id="HNX70833.1"
                     /translation="MTADSPASPSLFSRQMLICVFTGFASGLPLYLLINLLPAWLRSE
                     GVDLKAIGLFALIQLPYTWKFIWSPLMDRFTPPFLGRRRGWMLITQVALIASMAGFGV
                     FLPQQDIWSIAALAVGVAFFSASQDIVLDAYRRELLRDAELGLGNTVHINAYRLASLV
                     PGSLSLILADRLPWPWVFAITALFMLPGLLLTLSVSEPALRAAAPKTLRDAVVMPFKE
                     FISRQGWQGAAWVLGFIFLYKLGDSMATALATPFYLDTGFSKTEIGLVAKHAGLWPAV
                     IGGLLGGAWMLKLGINRALWVFGVVQCVSILGFVLLDQVGPDLRVLALAIGFEALGVG
                     LGTAAFIAFIARSTHPAYTATQFALFTSLAAVPRTVANAATGYLVDALGWTPFFLLCT
                     VLALPGMLLLYKVAPWGDDPPAQEADAPRQSR"
     gene            8849..>10101
                     /locus_tag="PKN55_06040"
     CDS             8849..>10101
                     /locus_tag="PKN55_06040"
                     /inference="COORDINATES: protein motif:HMM:NF019874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNX70834.1"
                     /translation="MFNRLSVGSKLNVLLLAALAGVALISALALYSKYQRMLEDRRAA
                     LQNMVQVAVGVLNYHDRLVRDGSLPLASAQAQARRAIGELRYADKEYFYIFDMHPRMV
                     QHPIKPELNGQDISQIRDPDGKPLFIAMRDVVSSQGHGFVEYQWPQPGQSAPVPKLAY
                     AQGFAPWGWVVATGLYLDDLNAEFRRDAMMFVGKIVGLSLLLAALVVLIKRALTLPVR
                     ALVEAMRRVDEQHDLTCRAQLHSQDELGTVGRAFNHLLGNIQQAFSDVWGGSEQLAHR
                     ATRLSDHAHRLAGASAEQNHAVASSAAALEEISASIEEIGVMADALSRQADDTRAQTA
                     HGETVVSGLRDSLAQAGQCLRDEVGVSARQMADNMADITRMTAEVREIAEQTNLLALN
                     AAIEAARAGESGRGFAVVADEVRKLA"
BASE COUNT         1731 a         3229 c         3343 g         1798 t
ORIGIN      
        1 gccggcagca gcaaccagga ttgcaactcg caggcggcgc gggtgtcggc gtccagcacg
       61 gatagccagc ggcgcgggat cgccttgggg ccttggtgcg cgccggccag cataccggcg
      121 atggcgccgg tggtgtcggc gtcgccgccc cggttcacca cgtcgatcag gcagtcttcc
      181 agcgtgtcgg tgtcaaaaaa cgcctgaaac accgcttgca gggtgtccac gatatagccg
      241 ctggggttgt cgcagcggcg ggcgcgaaag ccaaacaccg ggtattgctg gatcagcggc
      301 tgcacatgct gttgcagcac ggtttttttg tcggcgccgg ccagcagggc gtgcagcatg
      361 ccggtcagcg tcaggcaggc ggcgtcggat agggggctgt ggtgggtgac gtgcgcttgc
      421 gccagcacgg cctcgcgcac ggtgtcggcg tccaggccaa aggtggccag cgcggccggc
      481 agcaggcgca tggcggcgcc gttgccggcg tcgtgttcgg agggcggcgc gcagggttcg
      541 ccagtgcggc gaaagcgcat caggttgcgg cgcacggtgt tgccgatgtc caccggcttg
      601 ccgcgcatcc attggtcaaa cgcctgcgcg gccagcaggg cgtccacccg gccgccggat
      661 aataagatgg cctcgcccag cgccaggctc atggtggtgt cgtcggtgac catgccgggg
      721 cgcagcttga gccagccgcc gccggtcagg tggcggtgca ggccaatctg gtggcggatt
      781 tcattggggg tgagaaactc caccgtggcg cccagcgcat cgcccaccgc caggcccaga
      841 tacgcgccca gcgcgcgggc ttccagcgtc agcggcgccg agggcgcggg gccaaactgg
      901 cgtatgcggt tcaggactcc gggccaaagc atgctggccg gtcgtgacag gcttggcagg
      961 agggcgccgg gcagacgtaa atcccttcgc gcgcgcacag ttgccggtag atgaactttt
     1021 tccatttcat gtccccgtcg ttggcctggg ccagcgccgg aaagttcagc gcaatcaatt
     1081 ggctgagctc gtggcggccg gtcaggccga ggtcttgcca caggtggtcg cggccggcgc
     1141 aggcggcggc cagaatctgc gccatccagc gcggctcggc atgctcgcca gcgcgatggt
     1201 ccagtaaaaa ttgcaccagc tcgtcccatt ccggcacgcg ctcgggcgag gcggccggcc
     1261 agggcgcggg caggcgcgca gtaggaaaat aagcggccag catggcggca aaatcattgt
     1321 ctgccaggcc cagttggggc ggcaggcaac ccaggcccgc gcgctgtccg gccaacaggc
     1381 tggccagcaa gctgcggttg ccttcgtcaa caggcgccgg gccagcgtcg ccttgcaaaa
     1441 ggctggcgca caacagggcg cggtgatgca agcggtcggg ggggatcatg gtggcctccg
     1501 gatcggacgg ccgcgctgac gcccaagggc gccaggcggc agacgcggac ggctgggagt
     1561 cgcaggacgc cgtccgctca ggcaacatca cttgctgggg tattcgacca ggatgtcctc
     1621 gtcgcgcacc accatctggc aagccaggcg ccattgggtc ggcggaatgt cgtccacgta
     1681 catttggtcg agttccggct gggtcacata gcccatgtcc ttgagcacaa cggcttcgcg
     1741 gttggtcagc gggccgccca tcggcgaacg cttggtgtcc accgacgaca ccttgaccag
     1801 acaggtgccg cattcgccat tgccgcagcc aaagtcgatg ggaatgtggt tttctttggc
     1861 cagggtcagg atggtctggg tgtggctgcc agcgacggca tagacggttt tgtctttatg
     1921 cagacggctg gagaaggtga tcaacgccat ggtgttgctc cttgtggtga aaaaaccggc
     1981 agcgccaggt caacgcactg aaccatggcg cgcgcctggt ctgcgtgggg cccagactca
     2041 ggcaaagtac gaaacagcaa gtccgacagg cggcttcagg ccgggcgcac caccacttcg
     2101 ccgcccagaa tctgcgcctg acaggccaga cggtggttgc gcggggccat attgtctttc
     2161 agcacctgat cctccagcac cgagggcgcg gccaggttgt cgccgccaga caccaccttg
     2221 gtcaggcagg tgcagcattc gccttcgcga cagccatagg tgatgccagc gccgactttc
     2281 tcggacactt caatcaggcg ggtgccggcc gggacggtga ccgccacgcc gatgtcttca
     2341 aaaatcactt tggctttggg catggaatgc tccagtcagg ataaaaatca actcagggag
     2401 gccagatcga cctcacgcgg catggcttcg cccaccagat gacgggccag ggtttcgccc
     2461 agcgtgcggc aggcgtccag ctcgctggcg tcgggcgcca gcttcaggcg caggccttcc
     2521 accggcacgc gcagcttgag cccgcgcagg cggtcttcaa tcagccgcac cgcctcgccg
     2581 ctccagccat acgagccaaa cgccgcgcca aacttgcctt tgacgttcac cgtggtcagc
     2641 gaggacagca gcgcccagat cggcggcgcg gcgtcgccgt tcagcgtcgg gctgccaaag
     2701 gccaggccgt cggcggcttc gatcaggtcg gtgaagtggg cggtgtcgcc gccctccagg
     2761 tcaaacagcg acacgcgcac gccggcaatg cgctcggcgc caatggcgat ggcttcggcc
     2821 atgctgcggg tgttgccgta ggcggagaga taaaacaccg ccagggtttt ttcgccggcg
     2881 gcttcggtgt ccagcgccag gcgcggggtg gccatttcgc ggtagcgcgc caggtagtcg
     2941 cgcgggtggt ggcgcaaaat cggcccgtgc gccggggcaa tcaagcggat gtccagcggc
     3001 tccagaagct ccagcgcgtc cagcacatgg gcgcggaacg ggcgcatgat gtgggcgtag
     3061 tagtattcaa aagagaacct gaaatcgccc acccggtcgt tgaacaggcg cgggtcgcag
     3121 taatggcagc caaacacatc gcccgaaaac aacatgcctt cttccaccag ccaggtgcac
     3181 tgcgtgtccg gccagtgcag atacggcgtg tgaaaaaacg ccagcgtgcg cccgcccagg
     3241 tcgatggtgt cgccagtggt caccggggtg tagtccggcg gcgcctggtt ggacgagggc
     3301 ttgagcagcg ccttgagcat catttgcgcg cggctggaca aatacacgcg ggcattgggc
     3361 gcgcggcgca atagctcggg cagcgcgccg ctgtggtcgg gctccaggtg attgagcacg
     3421 atggtggtga tttcgtcgta acgggcgacg gattccagcc gacgaaaaaa atcgtcggca
     3481 aaggcttctt tgaccgtgtc gatcaccgcc acgccctgct cgccgcgcac caggtacgcg
     3541 ttgtagctgg tgccgttggc ggtggacaaa ataatgtcaa agcggcgcaa atgcgggtcc
     3601 agcgcgccaa tccaatgcac gccgggggcc agctccacgg cctgatcagg gtgaacaatg
     3661 ctcatgggcg tggccctccg cgtggcaggc ggcggcgtgc gccgccgcct cggcgccgtg
     3721 atgggaacag gcttccaggt cgcccggcgg caggttgacg ccattcaggc cgatgcgcgc
     3781 atccagcgcg gcgacgtcaa agcccaccca gcgctcgtcg cccatttcca gcaaggggcg
     3841 gcgaatcagg atggcgcgct tgaccatcaa ggccagcgcg gcgtcggcgt ccagcgcgtc
     3901 ggggttgacc tcgccggact tcactgccgg ggcgttgcgg ttaaaccact gcgccaccgg
     3961 cagcgcgcca aaaaattggc gcagccgctc gggcgtccag ggttcggtca gcagattgcg
     4021 cacatgcagc tggtggccgg cggcgcgcag cagggctttc tgccgcgcgt tgccctggca
     4081 gcctggcttt tcgtaaaaag tcagtgtcgc cattacgccg cctcccgatc cagcaagccc
     4141 agctcgcgca gcttgtccag cgggatgccg gtcagcgaac cgggcgggtt cagcggttcg
     4201 cccagttcgt tgacaatcgc cccctcaatc ggacaaatcg ccgcgcactg cgcttcggcg
     4261 tggtcgccca ggcactcggt gcaggcttgc tcgttgatca agaagtgcgg gacatcttcc
     4321 accacggctt gattggggca gacatccacg cacgcccagc agttgacgca cagatcgaca
     4381 atttgcagag ccatttacgc caccttgttc agcttgtcgg ccggcgccgc cagcttgccg
     4441 gcggcggaca tttcccgata caccgccagc acggcgtctt ccaccggctc catggcgtgt
     4501 tcgccattgg gctggatgcc ggcggcctcc agctggcccc agggctcaaa cccaatccgg
     4561 gcgcacagca cctgctcaca gccggacagg gcgtggatga tctggtcaag aatcgacacg
     4621 gccggctggc cgccttcgcc gctgtcgcca cagtcggtgg cgccgttgca gtacaggtcc
     4681 accttgcgat ggcccatcag ccgcacgccc ttgggcgagg cttcgtacac caagaattcg
     4741 cgggcatggc caaagtgctc gcccaccacg ccatttttgg cggccaccgc catcagcacc
     4801 gggcggctgt cggccggcac gccggccagg gcggtgactt cggcggccgg ggcgccggcg
     4861 cgcttggcgg ccagttgctc cagcaggttt tcgcggactt cctgacgggt ttgcatggcg
     4921 gcttcgtagt cgatgtccat gtcggccagc ttgtcgaggg tgaactcggc gccgcgatct
     4981 tcgcccagca agcccaccgc gtcggcgcgg cactggcgac agtggcgcat catcgccata
     5041 tcgccgccgc actcgtcttg cagggcttgc agctgttcgg gcgtgggcga cggctgctcc
     5101 atcaggccgt aataggtgcc gtgctcggct tcggcaatca gcggcatgac gttatgcaag
     5161 aaagcgccct tggccttgac caccttggag acttctttca ggtgctcgtc gttgacgcca
     5221 ggaatcatca ccgaattgac cttcaccaga atgccgcgcg aagtcagcat ttccaggcct
     5281 ttttgctgct gttcgatcag aatccgcgcc gcttccaccc cgcgcaaccg gcggttttgc
     5341 cagtaaatcc acgggtagat ctgggcgccg atttcgggat cgacacagtt gatggtgatg
     5401 gtcacatggt cgatgttgtg cttggcgatt tcgtccacat gctcgggcag ggtcaggccg
     5461 ttggtggaca ggcacagctt gatgtccggc gccttgtccg acagctgccg gaaagtctcg
     5521 aaggtgcgcg ccgggttggc cagggggtca ccggggccgg cgatgcccag cacggtcatt
     5581 tgcggaatgg tggccgccac ggccagcact ttggtgacgg cctgatccgg gtgcagcaac
     5641 tcggacacca cgcccggccg cgactcattg gcgcagtcgt atttgcggtt gcagtagttg
     5701 cactggatat tacaggccgg cgccaccgcc acatgcatgc gggcgaagta atggtgagcg
     5761 tcttctgaat aacagggatg atcctgcact ttctggcgga tggaatccgg cagatgggcc
     5821 atggcgtcgg acgacgaccc acagccgctg gacgaacagc cgccgccgct gctgggggcg
     5881 ttgctgataa caggtagttc cacgatgaca ctcctcaagt gctggtgctg acaagcactt
     5941 gagcaaaagc cgcgccacgt ggattaccta ttcaaatcaa tattttgcct attgatcttc
     6001 tgtccgtgtc gcatcacttg tcgtgaaccg gacaaggttg gcgtacgttt gcgtatccgc
     6061 cacggcatga ccgcgcagac ggccatcggc cagcacgcgc ccgccctggc gcagatacag
     6121 cccgcgcacc gccttgacgt attgctcggt ttcttcatac ggcggaatcc gccccagccg
     6181 ctgcaccacg ccctcgccag cgttataggc ggccagcgcc aaatccagcc ggcggtcgaa
     6241 caggcccagc aaaaaggcca ggtagcgcgc gccggcatcc agattggcgc gcggctccag
     6301 cacggtctcg gctgacacgc caaagcgtgc ggcggtgtcc ggcatcagtt gcatcagtcc
     6361 ctgcgcgccc ttgggcgagg ccgcgctggc gtggtagccg gattccacgg taatcaccgc
     6421 gtgcaacaag gcgggctcca gttggtggcg acgggcggtt tgctcgatca gcggcgtcag
     6481 ccgcttcagc gccgcctggt cgcgcgcgtc cagataacgg cccagttcgg cggtcgccgc
     6541 gctgctgctg gccgccggca gcgcaccgcg cttgaatagc cgatagcgcg catccagcgg
     6601 cttgtcggcc agatgcacct cgccctgttc gtccacaaag ccatagacat cggcgtgcgc
     6661 ctgccaggcg gccaggccca gggtgacaaa aaacagggtg cgcaggaaaa ctgtcatgtg
     6721 aatttaaggt ccaaaccgct atgctgcatc agctgcgcgc cggaagcccc gacgcttgcg
     6781 cgttcgcccg gaaaaatcgg cgacgccccg gcaatgtaag gacaatcccg gctattttca
     6841 atggtttatc gcaaggagag tgatatgggt ctgctggatc aactgagtga cgccatcagt
     6901 ggcggcgtgc aacagctgca agataccgtc ggccaggccg gcggcgcgct gggtgatttg
     6961 ctgcagaaca gcggcggcat cgatggcctg gtggagcgtt ttcagcaagc cgggctgggc
     7021 gacaccctgc aaagctggat cagccagggc gaaaacctgc cgatcagcgc cgagcaattg
     7081 cagcaggtgt tcagcgccga cacactgaac agcctggcag aaaaagccgg tatcgatccg
     7141 gcacaggcgg gtgagtggct gtcggccaat ctgccggaat gggtgaatca gctgaccccg
     7201 aatggctcgc tgtctgatct gggtggggtg tttgagcaag gcgtgtcggc ggtgaaagat
     7261 tttttcagca agttttgaat ctcgtcctat tccctgaccc cattccatcg cccatcctcg
     7321 gggtggccca acggccaccc cgcatcaccc gctgccgggt cacccccggc caccgcgcgc
     7381 ccgcaagcga catgattccg gtacagtagc agtctggctt attttttcgc cctgcccatg
     7441 actgctgatt cccccgcctc gccctcgctg ttttcccgcc agatgctgat ctgcgtgttc
     7501 accggctttg cctccggtct gccgctgtat ctgctgatca atctgctgcc cgcctggttg
     7561 cgcagcgaag gcgtggatct caaggccatt ggcctgtttg ccctgatcca gctgccctac
     7621 acctggaaat ttatctggtc gccgctgatg gaccgcttca ccccgccatt tttgggccgg
     7681 cggcgcggct ggatgctgat cacccaagtg gccttgatcg ccagcatggc cggctttggc
     7741 gtgtttttgc cgcagcagga tatttggtcg attgccgcgc tggcggtggg cgtggcgttt
     7801 ttttccgcca gccaggacat cgtgctggac gcctaccggc gcgagctgct gcgcgatgcc
     7861 gagctggggc tgggcaacac cgtgcacatc aacgcctacc ggctggccag tttggtgccc
     7921 ggctcgctgt cgctgattct ggccgaccgg ctgccctggc cgtgggtgtt cgccatcact
     7981 gcgctgttca tgctgccggg cttgctgctc accctgtcgg tcagcgagcc ggccttgcgc
     8041 gcggcggcgc caaaaaccct gcgcgacgcg gtggtgatgc cattcaaaga gtttatttcc
     8101 cgccagggct ggcaaggcgc cgcctgggtg ctgggcttta tctttttgta caagctgggc
     8161 gacagcatgg ccactgcgct ggccacgccg ttttatctgg acaccggctt tagcaaaacc
     8221 gaaatcggcc tggtggccaa gcacgccggg ctgtggccgg cggtgatcgg cggcctgctg
     8281 ggcggggcgt ggatgctcaa gctgggcatc aaccgcgcgc tgtgggtgtt tggcgtggtg
     8341 caatgcgtgt cgattctggg ctttgtgctg ctggaccagg taggcccgga tttgcgcgtg
     8401 ctggcgctgg cgattggctt tgaggcgctg ggcgtggggc tgggcacggc ggcgtttatt
     8461 gcctttatcg cccgcagcac ccacccggcg tacaccgcca cccagtttgc cttgttcacc
     8521 agcctggccg ccgtgccgcg caccgtggcc aacgccgcca ccggctatct ggtggatgcg
     8581 ctgggctgga cgccgttttt cttgttatgc actgtgcttg ccttacccgg gatgcttttg
     8641 ttatataagg tggccccatg gggagacgat ccgcccgcac aagaggcgga cgccccaagg
     8701 caatcgcgct gacgcctggt cagccgcata cagacaaaaa ccgagcaagg acgtcaggcc
     8761 tgccccatct ggcgggcccg ctgaggaggc ggccgatgct cgcaccatcg cccggcccat
     8821 gtcgccgggc gtagagagag acatcaagat gttcaaccga ctcagtgtgg gcagcaagct
     8881 caatgtcttg ctgctggccg ccttggccgg cgtggctttg atcagcgcgc tggccttgta
     8941 ttccaaatat caacgcatgc tggaagaccg tcgtgcggcc ttgcagaaca tggtgcaagt
     9001 ggccgttggc gtactgaact accacgaccg gctggtgcgc gacggcagtc tgccgctggc
     9061 cagtgcgcag gcgcaggcgc gccgcgccat cggtgaattg cgctacgccg acaaagagta
     9121 tttttatatc ttcgacatgc acccgcgcat ggtgcaacac ccgatcaaac ccgaactcaa
     9181 cggccaggac atcagccaga tccgcgaccc ggacggcaag ccgctgttca tcgccatgcg
     9241 cgatgtggtg agcagccagg ggcacggctt tgtcgaatac caatggccgc aaccaggcca
     9301 gagcgcgcca gtgcccaagc tggcctatgc ccagggcttt gccccgtggg gctgggtggt
     9361 ggccacgggc ttatacctgg acgacctcaa cgccgagttc cgccgcgatg ccatgatgtt
     9421 tgtcggcaag atcgtcggcc tgtcgctgct gctggcagcg ctggtggtgc tgatcaaacg
     9481 cgcgctcacc ctgccggtgc gcgcgctggt ggaggccatg cgccgggtgg acgagcagca
     9541 cgacctcacc tgtcgcgcgc aattgcacag ccaggacgag ctgggcacgg tgggccgcgc
     9601 ctttaatcac ttgctgggca atatccagca ggctttttct gatgtgtggg gcggcagcga
     9661 gcaactggcc caccgcgcca cccgcctgtc cgaccacgcc caccggctgg ccggcgcctc
     9721 ggccgaacaa aaccacgcgg tggcctccag cgcggcggcg ctggaagaaa tcagcgccag
     9781 catcgaagaa atcggcgtga tggccgacgc gctcagccgc caggccgacg acacccgcgc
     9841 gcaaaccgcc cacggcgaaa ccgtggtcag cggcttgcgc gacagcctgg cgcaagccgg
     9901 ccagtgcctg cgcgacgagg tgggcgtcag cgcccgccag atggccgaca atatggccga
     9961 catcacccgc atgaccgccg aagtgcgcga aatcgccgag caaaccaatc tgttggcgct
    10021 gaacgccgcc atcgaagccg cccgcgccgg cgaatccggg cgcggctttg ccgtggtggc
    10081 cgacgaagtg cgcaaactgg c
//
LOCUS       DAOPYX010000086         9953 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_7953_length_9953_cov_12.302384, whole genome shotgun sequence.
ACCESSION   DAOPYX010000086 DAOPYX010000000
VERSION     DAOPYX010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 9953)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9953)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9953
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7953_length_9953_cov_12.302384"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..392)
                     /locus_tag="PKN55_06045"
     CDS             complement(<1..392)
                     /locus_tag="PKN55_06045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011136825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HNX70835.1"
                     /translation="MNTIRYTAPARLLHWLMAALLIGLLALGFYMSDLPLSPEKLQLY
                     AWHKWAGVTAFILLAIRLLWRATHRPPALPASMSAAAQRMAHLGHLALYALMIAIPLS
                     GWLMSSAKGFQTVYFGVLPIPDLLAKNP"
     gene            complement(449..1258)
                     /locus_tag="PKN55_06050"
     CDS             complement(449..1258)
                     /locus_tag="PKN55_06050"
                     /inference="COORDINATES: protein motif:HMM:NF014899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class III extradiol ring-cleavage dioxygenase"
                     /protein_id="HNX70836.1"
                     /translation="MNAPLPSLFVPHGAPTFALAPGAAGAALRDEAQRLPQPRAIIMV
                     SAHWDTAVPTVGVADRLDTIHDFWGFPEPLYEIRYPASGCREAAGEVAAALSAAGLPV
                     QQDAERGLDHGAWIPLRLMYPHAEVPVIPLSLVSHGGPQLAWQMGRALAPLTRQGFLV
                     IGSGNLTHNLRDFQRAYAQGGSGSDYVKQFTDWLEKHAAAGDIAALLDYRRQGPGGAQ
                     AHPSDEHLLPFYVALGAAGEGAQAERFHAGIDHYVIAMDAYRFTPRGTELS"
     gene            1377..2252
                     /locus_tag="PKN55_06055"
     CDS             1377..2252
                     /locus_tag="PKN55_06055"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HNX70837.1"
                     /translation="MDRFAAMRLFVRVAELGSFSAVAQQMGVARSVVTRQVAALEQHL
                     GCKLMARSTRSLTLTSAGAAYLEKCRVILNLVEAAETDIASERQTPRGPIRISLPLSF
                     GVKRVAPLLLEFARRHPEVTLDMDYSDRRVNLIEEGIDLSIRVTRQLASGDVARRLGG
                     CVNRVVAAPDYLARHGCPHHPRELAHHAFLGYTLSNSMVSFDIDGQLLNVPVRPQLTA
                     NNGEVLAEAAAQGMGITSQPDFIVEDWLASGRLEVILRDFAMPPLGVYALLPGNKQIP
                     HRVRVLLEFLAERMG"
     gene            2545..3093
                     /locus_tag="PKN55_06060"
     CDS             2545..3093
                     /locus_tag="PKN55_06060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX70838.1"
                     /translation="MSDLSLQHHLTHPQALHEALASLSQRHALTVVIADRDFIDAAPG
                     RASECRARALALAALCQASGAPLRCALESLLPPQRGLAHALQARLDGEAGHACAAARL
                     AALQLALGAGGVHAPDELSERVDALLGDVRPSGHGLVLRLSLETAAAPAPADEAGVLA
                     RLLADSQAVAAQMRAQALTHQE"
     gene            3100..3405
                     /locus_tag="PKN55_06065"
     CDS             3100..3405
                     /locus_tag="PKN55_06065"
                     /inference="COORDINATES: protein motif:HMM:NF018717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1330 domain-containing protein"
                     /protein_id="HNX70839.1"
                     /translation="MKKAYWIVQVDMTQPEAFAAYVAANGEALARYGARFLARAGEHC
                     VVEGQARARNTIVEFPSYQAALDCWHDAAYQAARQLRHGAALMDVVIVQGYDGPQPA"
     gene            complement(3622..4161)
                     /locus_tag="PKN55_06070"
     CDS             complement(3622..4161)
                     /locus_tag="PKN55_06070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX70840.1"
                     /translation="MEPIKTTFKGKQVIKLKSGETISLIELPKPGISSTQTVVRIDKS
                     GDLIVGDLAHTRHHTWLEGGIVDGKPTPDLAGWKADLKVLPTLGKGKVYGGRGDFVPV
                     KEAVAQEVAYLEKADQIVDGYLKALGPRVSELNDSEKKGAHFAAIQAEFVKAFPDYAF
                     PDMVGYSVYGLVQHKLTSH"
     gene            4203..4307
                     /locus_tag="PKN55_06075"
     CDS             4203..4307
                     /locus_tag="PKN55_06075"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX70841.1"
                     /translation="MALAVGYQDVSAFIQVFKDAFGDTPARYARDRRA"
     regulatory      4355..4565
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            4606..8970
                     /locus_tag="PKN55_06080"
     CDS             4606..8970
                     /locus_tag="PKN55_06080"
                     /EC_number="6.6.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF014563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobaltochelatase subunit CobN"
                     /protein_id="HNX70842.1"
                     /translation="MYFPLSVSRARVWLGVCLLLCLCALPWPTHAGVLLAVVSERAAP
                     TALAAARRHLAQHPQDRVLLRTPAQWQQASAREQDAWLRSADSVLAVAVFGDAARALK
                     TSLARSLPPQANLLAFNGEASLSLQSRWRGDSLRDTPPELLRDLSQETPPPAALSAAE
                     RQPLLARWLRARQWWQAGGADNAAALFAYALRPDAAAPAPQPEPAFRLRAQGREQRAE
                     LSGEGWLRLGATLGLRAAPTVLVLDLANAETDSADALCRAIEARGLACAALFSRWGGA
                     SRQALEQLPALLAPAQPAALVVMQDFVVGAADGREAVSAALTRLNVPVFKAIRLTERS
                     QTQWRLSSDGLPADSVQYRVALPELQGIGQPHVISALGPAQRDAATGLALRRPQALPA
                     EIQRLASRVARWHTLRSRANADKRVAIIYYNHPPGRQNIGADNLDVPNTLFDLLHRLQ
                     AAGYTTGPLPASPQALLDQIMAHGVNLPEDAAGLAAMSGQVAGMSAADYARRLARLPA
                     RVRDEMQYGPLGRLHAEVLEAEAAGEWALGRKRVEGALKELHHLLSGVSHPQRELALA
                     QLQTLQAGYLRCLAEARQPRPTPCARLGGLAQQLSRHEIEGLRGWGASPGRVMVSRGR
                     LVFPGLRFGQVFIGPQPPRGWEVDEELLHANTSITPPHQYLAFYQWLRDDFAADVLVH
                     VGRHSTYEFLPGKAVGLAADDYPSLIADDLPSVYPYIVDGVGEGTQAKRRGQAVIVDH
                     LTPPLAATPLYDRLLNLRQMVESYEAASSDSLKAQAAQEMRAQVQALNLRAELEASMA
                     DVLEVRGIGFDAADDDLLAHEIGHYLTKLQEKFMPHGLHVFGRPWSDASLQLMLDSMR
                     QGGVDADAAAGPLRDSPAAEMDSLLNALAGGHVPPGKGNDPLRSPEALPTGRNFHAVD
                     GDLLPTRLGYALGARMAARAADGEGSEGVILWASDAVRDEGVMVGFALALMGAEPVWN
                     ARGIVTDVRLLPDARRRDVIVTTSGLFRDLYPHLLRLIDRAGRLALAASAAPLRQAHP
                     ELAPALDAALAPVPGAEAGEQPLADNRVAQQWLRRLRALVAQGQPLADAGRAAALRLF
                     GDAPGAYGAGVNRLTERSAAWRERDELARAYRLRMGHGYGLDADGEASHAALDAALDG
                     IARTYHGRASHLYGLLDNNDAFDYLGGLSLAVESRVGAPPQAWILQHADPGRAGLEPL
                     ASALLGELRGRFLNPAWLRPLMAHGYAGARTMGQEFLENLWGWQVTRPDLVQSWAWDE
                     VKSVYFDDAHQLGLPRFLAEGHNAHAKAHMLAIMMVAAQRGYWRADADSVRRLGGELA
                     QLVARNGLPGSGHTSPHHPMWAWLAEQLSADDAQALQGALSSARGADSSAGETPTPSA
                     AALPRAAHTPTPRGARPQASAADAPAPLNPPRWRELRLSPPPAAPVFDAPWAWLAGLG
                     ALLLALGLWRGLRSPHPV"
     gene            8989..9414
                     /locus_tag="PKN55_06085"
     CDS             8989..9414
                     /locus_tag="PKN55_06085"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HNX70843.1"
                     /translation="MLSLDWLHAMVSYLLPPALWALLLACAVAVCDLAVALGERLRGL
                     RQLRAAGQPQHALALARRRLERADLLARLPPMLGLMATIIPLGPGLAALGQGQPALLA
                     SAITTAFDATVLGLAAGMVGLVLGKLRRRWYEELLEEMA"
     gene            9411..9722
                     /locus_tag="PKN55_06090"
     CDS             9411..9722
                     /locus_tag="PKN55_06090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX70844.1"
                     /translation="MKPRLQLYRRLDAALGDEHEDPRASLVNLVDVMLVFACGLLAAL
                     TASQPSALSRPQPVEKGREIAQPEHAPGPGAGYDRVGEVFRDPVSGKLVLIEAPRTDK
                     P"
BASE COUNT         1549 a         3401 c         3381 g         1622 t
ORIGIN      
        1 tcggggtttt tggccagcaa gtccggaatg ggcagcacgc cgaaatacac ggtctgaaag
       61 cccttggccg agctcatcag ccagccggac agcgggatgg cgatcatcag cgcatacagc
      121 gccagatggc ccagatgcgc catgcgctgc gccgcagccg acatgctggc cggcagcgct
      181 ggcgggcggt gggttgcacg ccacagcaag cgaatcgcca gcaagataaa cgccgtcacg
      241 cccgcccatt tgtgccaggc atacagttgc agtttttctg gcgacagcgg caggtcgctc
      301 atataaaagc ccagcgccag caggccaatc agcagggcgg ccatcagcca gtgcagcagg
      361 cgggccgggg cggtgtagcg aatggtgttc atggtgtgct ccttggggcc ggggtgttgc
      421 ccctgtggtg tttccgtgtg ctgtgatgtc agctcagttc ggtgccgcgc ggggtaaagc
      481 ggtaggcgtc catggcaatc acgtagtgat caatgccggc atgaaaacgt tctgcctgcg
      541 cgccctcgcc cgccgcgccc agcgccacat aaaacggcag cagatgttca tcgctggggt
      601 gggcctgcgc cccgccgggg ccttggcgac ggtaatccag cagtgcggcg atatctccgg
      661 cagcagcgtg tttttccaac cagtcggtaa actgcttcac gtagtccgag ccactgccgc
      721 cctgggcgta agcgcgctga aaatcgcgca gattgtgggt caggttgccc gagccaatca
      781 ccaaaaagcc ctggcgggtc agcggcgcca gcgcgcggcc catttgccag gccagctgcg
      841 ggccgccgtg gctgaccagc gacagcggaa tcaccggcac ctcggcgtgc ggatacatca
      901 gccgcagcgg aatccaggcg ccgtggtcca gcccgcgctc ggcgtcctgc tgcaccggca
      961 agccggcggc gctcagggcg gcggccactt cgcctgcggc ttcgcggcaa ccgctggccg
     1021 ggtagcggat ttcatataag ggctcaggaa acccccaaaa atcgtgaatg gtgtccagcc
     1081 ggtcggccac gccgacggtg ggcacggcgg tgtcccaatg ggcgctgacc atgatgatgg
     1141 cgcgcggctg cggcaggcgc tgcgcctcgt cacgcagcgc cgcgccggcg gcgccgggcg
     1201 ccagggcaaa ggtgggcgcg ccgtggggca caaacaaact gggcaggggg gcgttcatct
     1261 tggggctcgc tttcttggcg gcggtgtgtc cgtgttgtcg atgggtgaac tgtagacttc
     1321 ttttttgagg cgaaatagcc gacaatccag aaatcactat tgctggattg gcaacaatgg
     1381 atcgctttgc cgccatgcgc ctgtttgtgc gggtggccga actgggcagc tttagcgctg
     1441 tcgcccagca aatgggcgtg gcgcgctcgg tggtcacccg gcaggtggcc gcgctggagc
     1501 agcacctggg ctgcaagctg atggcgcgca gcacgcgcag cctgacgctg acctcggccg
     1561 gcgccgccta tctggaaaaa tgccgggtga ttttgaacct ggtggaagcc gccgaaaccg
     1621 atatcgccag cgagcggcaa accccgcgcg ggccgattcg catcagcctg ccgctgagtt
     1681 ttggcgtcaa gcgggtggcc ccgctcttgc tggaattcgc ccgccgccac ccggaagtta
     1741 ccctggacat ggattacagc gaccggcggg tgaatctgat tgaagaaggg attgatcttt
     1801 ccatccgcgt cacccgccag ctggccagcg gcgatgtggc gcggcggctg ggcggctgcg
     1861 tcaaccgggt ggtggccgcg ccggattacc tggcccgcca cggctgcccg caccacccgc
     1921 gcgaactggc ccaccatgcg tttttgggtt acacgctgtc caacagcatg gtgagctttg
     1981 acatcgacgg ccaattgctg aacgtgccgg tgcgcccgca gctcaccgcc aacaacggcg
     2041 aagtgctggc cgaagccgcc gcgcaaggca tgggcatcac cagtcagccg gattttattg
     2101 tcgaagactg gctggccagc ggccggctgg aggtgatttt gcgcgacttt gccatgcccc
     2161 cgctgggggt gtatgcgctg ctgccgggca ataaacagat tccgcaccgg gtgcgggtgc
     2221 tgctggagtt tttggccgag cggatgggat gaggcccgca cgggctgaca gcctgcgctt
     2281 gacaagcccg gcccgcccgg cgcaagatgg ccgcactatg gcacagcaca ccctccacgc
     2341 acattcgtat tattggtggc gcaccgactc cgggcgtcac tcgtttgcgt attgctgaaa
     2401 gccagcaccg aatccacgca acgcatgccg ccccggttgg gcggcatgtg tgttttcagg
     2461 gtttgacttc tgcgcaccgc gcgcacccgc accccaccac acccaggctc ccgactgcga
     2521 caccctacac aggagcctga aaccatgtct gatctgagcc tgcaacacca tctgacccac
     2581 ccgcaagcgc tgcacgaagc cctggccagc ctgagccagc gccacgcgct gaccgtggtg
     2641 attgccgacc gcgactttat cgacgccgcc cccggccgcg ccagtgaatg ccgcgcccgc
     2701 gcgctggcgc tggcagcgct gtgccaggcc agcggcgcgc cgctgcgctg cgcgctggaa
     2761 agcctgctgc cgccgcaacg cggcttggcg catgccctgc aagcccggct ggacggcgaa
     2821 gccggccacg cctgcgccgc cgcccggctg gccgcgctgc aactggcgct gggcgctggc
     2881 ggcgtgcacg cgccggacga gctgagcgag cgggtggacg ccctgctggg cgacgtccgc
     2941 cccagcggcc acggcctggt gctgcgcctg agcctggaaa ctgccgccgc cccggccccg
     3001 gccgacgaag ccggcgtgct ggcccgcctg ctggcggata gccaggcggt ggcggcgcag
     3061 atgcgcgccc aggcgctgac ccatcaggaa tgacacatca tgaaaaaagc ctactggatt
     3121 gttcaggtgg acatgactca gcctgaagca tttgccgcct atgtggctgc caatggcgag
     3181 gctctggccc gatatggcgc ccggtttctg gcgcgtgcgg gtgaacattg cgtggtagaa
     3241 gggcaggcac gcgcacgcaa caccattgtg gagtttcctt cgtatcaggc cgcgctggac
     3301 tgctggcatg acgcggcata tcaggcggcc aggcagttgc gccatggcgc cgcgctgatg
     3361 gacgtggtga ttgtgcaagg ttacgacggc ccgcagccgg cgtaagaacg cgttcaaagt
     3421 cggcgaaact cggatgatcc tgcgttgaaa tgcccctcaa tgcgcattga ccatctgtca
     3481 gctgcgcttt ttttgggcat tttgtcttga ctgacccgtc gttcgctaga ctttgaactc
     3541 gttctaagcg cggctaacaa cactcagcgc agtggccctg cccagcgtcg gctacatcca
     3601 tcgcgatgtg atgattcgcg ctcagtggga tgtgagtttg tgctgcacca aaccatagac
     3661 gctgtagccc accatgtcgg gaaaggcata gtccgggaag gctttgacga actcggcttg
     3721 aatggcggcg aaatgggccc ccttcttttc gctgtcgttc aactcgctca cccgggggcc
     3781 gagggctttc aggtagccat ccacaatctg atcggctttt tccagatagg ccacttcttg
     3841 ggccaccgct tcctttaccg gcacgaaatc accgcgaccg ccatagacct tgcctttacc
     3901 caaagtgggc agaaccttca gatcggcctt ccagccggcg agatcgggcg tgggcttgcc
     3961 gtccacaatg cccccctcca gccaggtatg gtgacgggta tgtgctagat cgccgacgat
     4021 gaggtcaccg ctcttatcga tgcgaaccac ggtctgggtg ctggaaatgc cgggcttggg
     4081 cagctcgatc aggctgatgg tctcgcccga cttgagcttg atcacctgct tgcccttgaa
     4141 ggtggttttg atgggttcca ccttgggata ggtttcatcg gtgaggcgaa agcgtcaccc
     4201 aggtggcact ggcggtgggc tatcaggatg tgtcggcgtt tattcaggta ttcaaagacg
     4261 cctttggcga cacccccgcc cgctatgcgc gcgaccggcg cgcctgagtt tcagctggcg
     4321 tggtttgccg tatctggccg ggcaatgctg ctatactgcg cgccgtgttg atggcgccgg
     4381 cggcaagccg gcttaaaagg gaatcccgtt caagtcggga gctgcccccg caactgtcat
     4441 cggcgagcca agcccctcgc gccactggat gcgcgtctgt gtgttgttca cagatgccgc
     4501 tgggaaggcg ggcgtcaggc cacgacccga gagccaggag acctgccaca acgcagtgat
     4561 ggactgttca ccagggcggg gtgtctctgg cataggcgac tttttgtgta tttcccctta
     4621 tctgtttcgc gcgcccgcgt ctggctgggc gtgtgccttt tgctgtgcct gtgcgccctg
     4681 ccctggccca cgcatgccgg cgtgttgctg gccgtggtca gcgagcgcgc cgcccccact
     4741 gcgctggccg ccgcccgccg ccatctggcc caacacccac aagaccgcgt gctgctgcgc
     4801 acgcccgcgc aatggcagca agccagcgcg cgcgagcaag acgcctggct gcgcagcgcc
     4861 gatagcgtgc tggcggtggc ggtgtttggc gacgccgcac gcgccctgaa aacctcgctg
     4921 gcgcgcagcc tgccgccgca ggccaatcta ttggctttta atggcgaagc cagcctcagc
     4981 ctgcaaagcc gctggcgcgg cgacagcctg cgcgacaccc cgcccgagct gctgcgcgac
     5041 ctcagccagg aaaccccgcc gcccgctgcg ctcagcgccg ccgagcgtca gccgctgctg
     5101 gcgcgttggt tgcgcgcgcg gcaatggtgg caggccggcg gcgctgacaa tgccgccgcg
     5161 ctgttcgcct acgcgctgcg ccccgatgcc gccgcgccag cgccgcagcc cgagccggcg
     5221 tttcgcctgc gcgcacaagg ccgcgaacag cgcgccgagc tgagcggcga aggctggctg
     5281 cgcctgggcg ccacgctggg cctgcgcgcc gcgcccaccg tgctggtgct ggatttggcc
     5341 aatgccgaaa ccgacagcgc cgacgcgctg tgccgcgcca tcgaagcgcg cgggctggcg
     5401 tgcgcggcgc tgttcagccg ctggggcggc gcctcgcgcc aggcgctgga gcaattgccc
     5461 gcgctgctgg cgccggccca gccggccgcg ctggtggtga tgcaggattt tgtggtgggc
     5521 gcggcggatg ggcgcgaggc ggtcagcgcg gcgctgaccc ggctgaatgt gccggtgttc
     5581 aaggccatcc gcctgaccga gcgcagccag acgcaatggc gcttgtccag cgacggcctg
     5641 ccggcggatt cggtgcaata ccgggtggcg ctgcccgagc tgcaaggcat tggccagccg
     5701 catgtgatca gcgcgctggg cccggcgcag cgcgacgccg ccaccggcct ggcgctgcgc
     5761 cgcccacagg ccctgcccgc cgaaatccag cgcctggcca gccgggtggc gcgctggcac
     5821 accctgcgca gccgcgccaa tgccgacaag cgcgtggcca tcatctatta caaccacccg
     5881 cccggccggc aaaacatcgg cgccgataat ctggacgtgc ccaataccct gtttgacctg
     5941 ctgcatcgcc tgcaagcggc cggctacacc accggcccgc tgccggccag cccacaggca
     6001 ttgctggatc aaatcatggc gcacggcgtc aacctgccag aagacgccgc cgggctggcg
     6061 gccatgtcgg gccaggtggc gggcatgagc gcggcggact acgcccgccg cctggcgcgc
     6121 ctgccggcgc gggtgcgcga cgaaatgcag tacggcccgc tgggccggct gcatgccgaa
     6181 gtgctggagg ccgaagccgc aggcgaatgg gcgctggggc gcaagcgggt ggaaggcgcg
     6241 ctgaaagaac tgcaccattt attgtctggg gtcagccacc cgcagcgcga gctggcgctg
     6301 gcgcaattgc aaaccctgca agccggctat ctgcgctgcc tggccgaagc gcgccagccg
     6361 cgccccacgc cgtgcgcgcg gctgggcggg ctggcgcagc agctcagccg ccacgagatc
     6421 gaaggcctgc gcggctgggg cgcgtcgcca gggcgggtga tggtcagccg tggccggctg
     6481 gtgtttccgg gcctgcgctt tggtcaggtg tttatcggcc cgcagccgcc gcgcggctgg
     6541 gaggtggacg aagaactgct gcacgccaac accagcatca ccccgccgca tcaatatctg
     6601 gcgttttacc aatggctgcg cgatgacttt gccgccgatg tgctggtgca cgtcggccgg
     6661 cattccacct atgaattttt gcccggcaag gccgtgggcc tggcggcgga cgactacccc
     6721 agcttgatcg ccgacgattt gcccagcgtg tatccgtata ttgtcgatgg cgtgggcgaa
     6781 ggcacgcagg ccaaacggcg cggccaggcg gtgattgtgg accacctcac cccgccgctg
     6841 gccgccacgc cgctgtatga ccgcctgctg aacctgcgcc agatggtgga aagctacgag
     6901 gccgccagtt cggacagcct gaaggcgcaa gccgcgcagg aaatgcgcgc acaagtgcag
     6961 gcgctgaacc tgcgcgccga gctggaagcc tcgatggccg atgtgctgga agtgcgcggc
     7021 attggctttg acgccgccga cgatgacctg ctggcccatg aaatcggcca ctacctgacc
     7081 aagctgcaag aaaaattcat gccgcacggc ctgcatgtgt ttggccgccc ctggtcggac
     7141 gcctcgctgc aactgatgct ggattccatg cgccagggcg gggtggatgc cgatgccgcc
     7201 gccggcccgc tgcgcgattc gcccgccgca gaaatggaca gcctgctcaa cgccctggcc
     7261 ggcggccatg tgccgcccgg caagggcaac gaccccttgc gctcgcccga ggcgctgccc
     7321 accgggcgca attttcatgc ggtggacggc gatttgctgc ccacccgcct gggctatgcg
     7381 ctgggcgcgc gcatggccgc ccgcgcggcc gatggcgaag gcagcgaagg ggtgattctg
     7441 tgggcgtccg acgccgtgcg cgacgaaggc gtgatggtcg gctttgccct ggcgctgatg
     7501 ggcgccgaac cggtctggaa cgcacgcggc attgtcaccg atgtgcgcct gctgcccgac
     7561 gcgcgccgcc gcgatgtgat tgtcaccacc tccggcctgt ttcgtgacct ctacccgcac
     7621 ttgctgcggc tgatcgaccg cgccggccgg ctggcgctgg ccgcctcggc cgccccgctg
     7681 cgccaggctc accccgagct ggccccggcg ctggacgccg cgctggcccc agtgcccggc
     7741 gcagaagccg gcgagcagcc gctggccgac aaccgggtgg cgcaacaatg gctgcgccgg
     7801 ctgcgcgcgc ttgtcgccca aggccagccg ctggccgacg ccggccgcgc ggcggcgctg
     7861 cgcctgtttg gcgatgcccc cggcgcctat ggcgcgggcg tcaaccggct gaccgaacgc
     7921 tcggccgcct ggcgcgagcg cgacgagctg gcgcgcgcct accggctgcg catgggccac
     7981 ggctacggcc tggacgccga cggcgaagcc agccacgccg ccctggacgc cgcgctggac
     8041 ggcattgccc gcacctacca cggccgcgcc agccatctgt acggcttatt ggacaataac
     8101 gatgcctttg attatctggg cgggctgtcg ctggcggtgg aaagccgggt gggcgcgccg
     8161 ccgcaagcct ggattttgca acacgccgac cccggtcgcg ccgggctgga gccgctggcc
     8221 tcggcgctgc tgggcgaact gcgcgggcgc tttctcaacc cggcctggct gcgtccgctg
     8281 atggcgcacg gctatgccgg cgcgcgcacc atgggccagg aatttctgga gaacctctgg
     8341 ggctggcaag tcacccgccc cgatctggtg caaagctggg cctgggacga ggtgaaatcc
     8401 gtgtactttg acgacgccca ccaactgggc ctgccgcgct ttctggccga aggccacaac
     8461 gcccacgcca aggcgcatat gctggccatc atgatggtgg ccgcccagcg cggctactgg
     8521 cgcgccgacg ccgacagcgt gcgtcgcctg ggcggcgagc tggcccaact ggtggcgcgc
     8581 aacggcctgc ccggcagcgg ccacaccagc ccgcatcacc ccatgtgggc ctggctggcc
     8641 gaacaattat ccgccgacga cgcccaggcg ctgcaaggcg cgctcagcag cgcgcgcggc
     8701 gccgacagca gcgcaggcga aacgcccacc ccgtccgccg ccgccttgcc gcgcgcagcc
     8761 cacacgccca ccccgcgcgg cgcgcgcccg caggccagcg ccgccgacgc ccccgcaccg
     8821 ctcaacccgc cgcgctggcg cgaactgcgt ctgagcccgc cgccggctgc gcccgtgttt
     8881 gatgcgcctt gggcctggct ggccggcctt ggcgcgctgt tgctggcgtt gggcctgtgg
     8941 cgcggcctgc gctcccctca tcctgtttga cttcggagaa cccacacaat gctatccctg
     9001 gattggctgc acgccatggt gtcttatctg ctgcccccgg ccctgtgggc cctgctgctg
     9061 gcctgcgccg tggcggtgtg tgatctggcg gtggcgctgg gcgagcgcct gcgcggcctg
     9121 cgtcagctgc gcgccgccgg ccagccacaa cacgcgctgg cgctggcgcg ccgccgactg
     9181 gagcgcgccg acctgctggc ccgcctgccg cccatgctcg gcctgatggc caccatcatc
     9241 ccgctggggc cgggcctggc cgcgctgggc caaggccaac ccgccctgct ggccagcgcc
     9301 atcaccaccg cttttgacgc caccgtgctc ggtctggccg ccggcatggt gggtctggtg
     9361 ctgggcaaac tgcgccgccg ctggtacgaa gaattgctgg aggagatggc atgaaaccgc
     9421 gcctgcaact gtatcgccgg ctggacgccg cgctgggcga cgaacacgaa gacccgcgcg
     9481 ccagcctggt gaacctggtg gacgtgatgc tggtgttcgc ctgcggcctg ctggccgcgc
     9541 tgaccgccag ccaacccagt gcgctcagcc ggccgcagcc cgtcgaaaaa ggccgggaaa
     9601 tcgcccaacc cgaacacgcc cccggcccag gcgctggcta tgaccgcgtg ggcgaagttt
     9661 ttcgcgatcc ggtcagcggc aagttggtgt tgattgaagc gccgcgcacg gacaaaccat
     9721 agccggtgtg aaccggccgc gttgggggaa gctggggatg gcggcggctt ggcatgaccg
     9781 ccatccgggc aaagcagggt tcagtgccgg gtgtcgccaa acacctcgtc cagcgacgcg
     9841 gcatccagca cccggtcgcc ccaggtttcc aactgggcag gcgtggcggc ggcaagtctg
     9901 gcctgctggg cggcggacag ttcgccaaaa cgacgggtga gctggcgttg caa
//
LOCUS       DAOPYX010000087         9933 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_7980_length_9933_cov_8.445637, whole genome shotgun sequence.
ACCESSION   DAOPYX010000087 DAOPYX010000000
VERSION     DAOPYX010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 9933)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9933)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9933
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7980_length_9933_cov_8.445637"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(30..884)
                     /locus_tag="PKN55_06095"
     CDS             complement(30..884)
                     /locus_tag="PKN55_06095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012696748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNX70845.1"
                     /translation="MRGHAFHVRHWGREGAPKLWMLHGWMDASPTFQFVVDALERDWH
                     VIAPDWRGFGLTAWNSGSYYFPDYLADLDALLSLHNPQAPARLVGHSLGGMVAMSYAG
                     ARPERVSRLVSIDAFGLLDIPPEHAPQRYRQWLQECAQPPGFAPVADLDHFASRLMRR
                     NPHLTATRARFLAEHLTGISADGVRRHLADPRHKMVNPVLYRLEEAKACWRAIRCPVQ
                     WILGGGASDHPLTGKIRQTLHERAACFHELEQLTIADSGHMVQQEQPEQVAAAIERFM
                     APTRGEAP"
     gene            complement(1087..2265)
                     /locus_tag="PKN55_06100"
     CDS             complement(1087..2265)
                     /locus_tag="PKN55_06100"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004285931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HNX70846.1"
                     /translation="MKTPLSFLLAATLLAPTLTFANTVAAPIAAPAAAPASFVPPPPE
                     IAGKAYFLNDFNSQLPIAVRDPDLRVDPASLTKLMTAYLTFKALREKRLSLEQTLLVS
                     AKGWKTEGSRMFLDPKTPVTVSDLIRGMIVQSGNDACVTLAEAIAGSEEVFGQVMTRE
                     ARRLGMNGTHFVNATGLPARDHYTTVRDLSILANAVIRDFPEFYPIYSMKEFRYNNIT
                     QPNRNLLLYRDPRVDGMKTGFTDAAGYNLIASARADGRRVVSVVVGTASPEARANESS
                     KLLNYGLQFFDSPRLYAANKPVGKLKVFKGEASEVNLGFTRDIYATVPKGSAARIQAK
                     LDAPAKLIAPIKAGQPVGKLTVTLDGKVLAEQPVLALNAVGEAGVFGRLVDSVKLWFN
                     "
     gene            2409..2996
                     /locus_tag="PKN55_06105"
     CDS             2409..2996
                     /locus_tag="PKN55_06105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018749575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HNX70847.1"
                     /translation="MPFTLAAPAHHQLIIKKSQFIACVEPMADRASAQARVAALRAEH
                     PGAAHVCWALLAGGQSAAVDDGEPSGTAGRPMLEVLRHQQLEGVLASVVRYFGGVKLG
                     AGGLVRAYTDSVAQALLSADKIAQVALCEGSATAAYPLEGVLRRELAQAGAQLLAVSH
                     ASEVCLRWRLPADQMPALSARLREVCAGRVQLTLD"
     assembly_gap    3045..3144
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3204..4223)
                     /locus_tag="PKN55_06110"
     CDS             complement(3204..4223)
                     /locus_tag="PKN55_06110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011136635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlxA family transcriptional regulator"
                     /protein_id="HNX70848.1"
                     /translation="MPVQPILPARRSSMPEPTPPIQKPLRYGFLLLPQFSLSAWALAA
                     DALTRANGLAERRVFETLTLSLEGERVRAGCGVDITTDLTLSYAPRLDALFIVAEDAQ
                     LPVDVELLQRHLALLSPDKLFIAGLDTGSYWMARAGLLSGYRATLHWQEINRFTEEFP
                     DVIVSSNLYEIDRNRGSCAGGAATLDFMLALIGQHMGADFAAQLSEVFSIERIRPGNE
                     RQRIPLQTRIGGSQPKLTEAVSLMEANIEEPLTTDDIAFYVGVSRRQLERLFKQHLNT
                     VPSKYYLELRLNRARQLLQQTSKSIVQIGLSCGFSSGPHFSSTYRNHFGITPREERAQ
                     RSQQG"
     gene            4761..9575
                     /locus_tag="PKN55_06115"
     CDS             4761..9575
                     /locus_tag="PKN55_06115"
                     /EC_number="1.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004352 - glutamate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0019551 - glutamate catabolic process to
                     2-oxoglutarate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-glutamate dehydrogenase"
                     /protein_id="HNX70849.1"
                     /translation="MSLSSQIELNALIADVAAIAAERLPAADHARLAPYFTAYFEEAE
                     AADLKRAAPLDLYGAAMAHLDFAGERLPGQHKVRAYNPDFERHGWQSTHTAIEIVNDD
                     MPFLIDSVAMLLARHNLTLHLLVHPVLEVERDADGRLLDARRTGERNLPLESLIHLQI
                     DRISDAAQLATVVAELEQVLNDIRLAVEDEPAMRRELQDIQAELQQAPLPDGKLDVNE
                     ISAFLDWVNARHFLLLGYCAYDLVRTDDGDALRIVPGSGHGILRNQGDKTYSASFAVL
                     PAHLRELAYDPACPIMLNKSQTRSTIHRSAHLDFIGIKRYDAQGHVIGECRFLGLYTA
                     AAYHESPRNIPILRRKMDAVMAECDYVENSYKEKTLQFVLESYPRDELFEIPVDVLQP
                     IAEGLVNLLERPRVRLFLRTDLYQRYVSALVFVPRDSFSTEVRLKIEKILMQALNGSA
                     AEYSVSISDTHLARVHYIIRTPGPLPAFDALGIEQDIARIVRGWGDELRQQLIDSHGE
                     ARGNTLYSQYQHAFPVAYREDFSPRHAVLDISLIEEALAGAPLALKLYKPLRKGSAGQ
                     NLKVFRAGQPVSLSASLPVLENMGVRVQDERPYAVERADGATVWINDFGLEVANVAHI
                     EQDDVRERFQQLLRRVAAGQCENDGFNKLALLADLDWREVILVRALAKYLRQAGLTFS
                     QSYVEQCLGNYPAITGGLVRLFLARLDPAHDDAGASARLEAEVREQFEQVANLDEDRI
                     LNGLLTIILAIRRTNYWQRDAAGQPKPYLSFKLESGAIPFLPQPRPLFEIWVYGARVE
                     GVHLRGSKVARGGLRWSDRMEDFRTEVLGLVKAQMVKNSVIVPMGSKGGFVCKQLPPA
                     SEREAWLAEGVACYKMFISGLLDLTDNLVNGQIAPPVAVRRLDEDDPYLVVAADKGTA
                     TFSDIANSVSEAYGFWLGDAFASGGSAGYDHKKMGITARGAWESVKRHFRHLGVNTQT
                     QDFTVIGIGDMAGDVFGNGMLLSEHIRLLAAFNHQHIFLDPNPDAAVSFAERARLFNL
                     PRSSWADYDSSLISDGGGVFERRAKSIPLSPAVRAWLGVERDSMAPTELIHAILQAPA
                     DLLYNGGIGTYIKASTQSHAEANDRANDGLRVNGNELRVKVIGEGGNLGITQLGRIEC
                     ARHGVALSSDAIDNSAGVDCSDHEVNIKILLGGVMQAGDMTLKQRNELLAEMTDEVGH
                     LVLRNNYLQTQILAVNKAHAAQMLSAQQRMISHMEKTGELNRALEFLPDDSAIAERRA
                     SRQGLTSPEVAVLLAYSKISLDRALQASALPDHPDLDSVLVNYFPSALQTRFADAMRR
                     HPLKREIIANQLANQVVNRMGTTFIFRMQEESAWPAADIVQAWVTVNRIFDGEALWNA
                     IEALDNRVPADVQVSMLVQVRTLIERATRWLLRSGRPLGDIAAAVARCRAPMAELLAG
                     LPGWIDAAHYPAVADWEQRLHAAHTPAELAGVVARLDFAVAGFDILELAQAQNLPLAV
                     VAANYFELGRVLELDWLRDAITRLPRDNRWQALTRSALRDDLYRLHRELTASALACPA
                     CGEAQYAKHWLAGKGGELEVCQQMLSELQGYDTLDLAMLSAGMREVGNHLMR"
BASE COUNT         1837 a         3134 c         3145 g         1717 t
ORIGIN      
        1 tggcaaacag gtgatccatt ggccgcgctt tatggcgctt cgccgcgtgt cggcgccata
       61 aagcgctcga tggcggcggc cacttgttcg ggttgttcct gctgcaccat atgccccgag
      121 tcggcgatgg tgagctgttc cagctcatga aaacaggcgg cgcgttcgtg aagggtttgc
      181 cggattttgc cggtcagcgg gtggtcgctg gcgccgccgc caagaatcca ttgcaccggg
      241 cagcgaatgg cgcgccagca ggctttggct tcttccagtc ggtagagcac cggattgacc
      301 attttatggc gcgggtcggc caagtggcgg cgcacgccgt cggcgctgat gccggtgagg
      361 tgttcggcca gaaagcgtgc gcgcgtggcg gtcaggtgcg gattgcggcg catcagccgg
      421 ctggcaaagt gatccagatc ggccaccggg gcaaagccgg gcggttgcgc gcattcttgc
      481 agccattgcc gatagcgctg cggcgcgtgt tcgggcggga tatccagcag gccaaacgca
      541 tcaatcgaca ccagccggct cacccgctcc gggcgggcgc cggcatagct catcgccacc
      601 atgccgccca ggctgtggcc caccagccgg gccggggcct gcgggttgtg caggctgagc
      661 agggcatcca ggtcggccag ataatccgga aaataatagc tgccgctgtt ccaggcggtg
      721 aggccaaatc cgcgccagtc gggggcgatg acgtgccagt cacgttccag cgcatcaacg
      781 acaaactgaa aggtgggcga ggcatccatc cagccgtgca gcatccacag cttgggcgcg
      841 ccttcgcgcc cccagtggcg cacatgaaag gcatggccgc gcacggtcag ccattcagaa
      901 cgtgatacgt attttggtgt atacataggc ggcattgtaa tacatggtgg atgctgctgc
      961 cgaaccatgc agggcgatcc caccggcacg acttacacgc ccttggaatc gccctgcatg
     1021 gtctgatgcc tgctggctac gccaggccat ctgcaaagat gacatggcaa cagtgtggtg
     1081 actgaatcag ttaaaccaca gttttacact gtccaccaag cgaccaaaca caccggcctc
     1141 gcccactgca ttcagcgcca gcaccggctg ttcggccagc accttgccat ccagcgtcac
     1201 cgtcagcttg cccaccggct ggccggcctt gatcggtgca atcagcttgg ccggggcatc
     1261 cagtttggcc tgaatgcgtg cggcgctgcc cttgggcacg gtggcgtaaa tatcgcgggt
     1321 aaagcccagg ttcacttcgc tggcctcgcc cttgaacacc ttgagtttgc ctaccggctt
     1381 gttggcggcg tacaggcgcg gtgaatcaaa aaattgcagg ccgtagttca gcagcttgct
     1441 gctttcgttg gcgcgcgctt ccgggctggc cgtgcccacc accaccgaca ccacgcggcg
     1501 gccatcggcg cgggccgagg caatcaggtt gtagccggcg gcgtcggtaa agccggtttt
     1561 catgccgtcc acgcgcgggt cacggtacag cagcaggttg cggttgggct gggtgatgtt
     1621 gttatagcgg aattctttca tcgaataaat cggatagaac tccgggaaat cccgaatcac
     1681 cgcattggcc agaatgctca aatcgcgcac cgtggtgtag tggtcgcgcg ccggcaggcc
     1741 ggtggcgttg acaaagtggg tgccgttcat gcccaggcgg cgcgcttcgc gggtcatcac
     1801 ctggccaaac acctcttcgc tgccggcaat tgcctcggcc agcgtcacgc aggcgtcgtt
     1861 gccggattgc acgatcatgc cgcgaatcag atcagacacc gtcaccgggg ttttcggatc
     1921 cagaaacatc cgcgagccct cggttttcca gcccttggcc gacaccagca gggtttgctc
     1981 caggctcagg cgtttttcgc gcagcgcctt gaaggtgagg taggcggtca tcagcttggt
     2041 cagcgaggcc gggtccaccc gcaaatccgg gtcgcgcacg gcaatcggca gctggctgtt
     2101 gaaatcattc agaaaatacg ccttgccggc aatctcgggc ggcggcggca caaagctggc
     2161 cggggcggcg gcgggcgcgg caatcggcgc agcaacggtg ttggcaaaag tcagcgtcgg
     2221 cgccagcagg gtggcggcaa gcaaaaaaga gagcggagtt ttcatgcaga aatcgggcgc
     2281 ggcgggcgcg ccatccgttg aggggacgag agcgtcagga ttatacaccg ctgaatccgg
     2341 tgcggggctt gtgttggcgc gccagcagaa tttacccttg cgcctttctg ccttctggcg
     2401 atgtgcccat gcccttcacc ctggccgccc cggcccacca tcagctgatc atcaaaaaaa
     2461 gccagtttat cgcctgtgtc gaaccgatgg ccgaccgcgc cagcgcacaa gcgcgggtgg
     2521 cggccttgcg cgccgaacac cccggtgccg cccatgtgtg ctgggccttg ctggccggcg
     2581 gccagtctgc cgcggtggac gacggcgagc ccagcggcac cgccgggcgg ccgatgctgg
     2641 aagtgctgcg ccatcagcag ctggaaggcg tgctggccag cgtggtgcgc tattttggcg
     2701 gggtgaaact gggcgccggc ggcctggtgc gcgcctatac cgacagcgtg gcgcaagcgc
     2761 tgctgagcgc cgacaagatt gcccaggtgg ccctgtgcga aggcagcgcc accgcagcgt
     2821 atccgctgga aggggtatta cgccgcgagc tggcgcaggc cggcgcacag ctgctggccg
     2881 tcagccacgc cagcgaggta tgtttgcgct ggcgtctgcc ggcagatcaa atgccggccc
     2941 tgagtgcgcg cttgcgcgag gtgtgcgccg gccgggtgca gctgacgctg gactaggcgt
     3001 cgctcaccaa accccgccca gcgatcctgg ccaggcgtgc agccnnnnnn nnnnnnnnnn
     3061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3121 nnnnnnnnnn nnnnnnnnnn nnnncctggc caggcgtgca gccaacacac ggcaacgacg
     3181 ccagggggtt ttgtgagcga cacttagcct tgctggctgc gctgggcgcg ctcttcgcgc
     3241 ggggtaatgc caaagtgatt gcggtaggtg ctggaaaaat gcggcccgga cgaaaacccg
     3301 cacgacaggc cgatttgcac aatggatttg ctggtttgct gcaagagctg gcgggcgcgg
     3361 ttcaggcgca gttccagata gtatttggac ggcacggtgt tcaggtgttg tttgaacagc
     3421 cgttccagct ggcggcgcga cacgccgaca taaaaggcaa tatcgtcggt ggtcagcggc
     3481 tcttcgatat tggcttccat caggctgacg gcttcggtca gcttgggctg gctgccgccg
     3541 atgcgggttt gcagcggaat gcgctggcgc tcgttgccgg gacggatgcg ctcgatgctg
     3601 aacacttcgg acaattgcgc ggcaaagtcg gcgcccatgt gctggccaat cagcgccagc
     3661 atgaaatcca gcgtggccgc cccgccggcg cacgagccgc gattgcggtc gatctcgtag
     3721 agattggacg acacgatcac atcggggaat tcttcggtaa agcggttgat ttcctgccag
     3781 tgcagggtgg cgcgatagcc gcttaacagc ccggcgcgcg ccatccagta actgccggtg
     3841 tccaggccgg cgataaacag cttgtctggc gacagcagcg ccagatggcg ttgcaacagc
     3901 tcgacatcca ccggcagctg ggcgtcttcg gccacgatga acagcgcatc cagccggggc
     3961 gcgtagctga gcgtcaggtc ggtggtgatg tccacgccgc agccggcgcg cacccgttcg
     4021 ccttccaggc tgagcgtcag cgtttcgaac acccgccgtt cggccagccc attggcgcgc
     4081 gtcagcgcat ccgccgccaa ggcccaggcg gacagggaaa actgcggcaa cagcaaaaag
     4141 ccgtagcgca ggggtttctg gatgggcggg gtgggttccg gcatggaaga gcggcgggca
     4201 ggcaggatcg gttgaacggg cacgtcagaa ctcacactag ggtaaatcgg caaggatggc
     4261 aagcgcgccg tggaaaggcg cgggcggcag gtaaaatggc ggaatgcgcc cggcgcaggc
     4321 aggtttcacc aaacccggca gggtagcggc gagcatcacc gcagcgcaag ggttgtgtcg
     4381 ggcattttgt gcttttatct ggccgtgtcg gggcaaaatc tgcccattgc cctcgctgcg
     4441 atgccgccag aatccgctcg cccggcgccc gcatgcctgc ggaatggccg ccgggccagg
     4501 tgggcgcgcc gatcgcccgg ctgtcatggc gacagcctga acgccagcta taaacagagt
     4561 gtcagcatac acggtgctga acaaatgtcg ctttcaacgg cccgtctcgt cttgatgttg
     4621 tatctccatg tcgcaaaacc gaaggtggca ggcttgagcc atgcctagaa tcggcgcatg
     4681 cccatctcct gtccggagtg gcgcatgcca agacacactc tgccccagct tgttgcctag
     4741 cactgaaaag agcccaacac atgtccttgt ccagccagat tgaactgaac gcgctgattg
     4801 ccgatgtcgc cgcgattgcc gccgagcgtc tgcccgccgc cgaccacgcc cggcttgccc
     4861 cgtatttcac cgcctatttt gaagaagccg aggcggccga cctcaagcgc gccgcgccgc
     4921 tcgacctcta cggcgccgcc atggcccacc tggactttgc cggcgaacgc ctgcccggcc
     4981 agcacaaggt gcgcgcctac aacccggatt ttgagcgcca cggttggcaa tccacccaca
     5041 ccgccatcga aatcgtcaac gacgacatgc cgttcttgat tgattcggtg gccatgctgc
     5101 tggcccgcca caacctcacc ctgcatctgt tggtgcatcc ggtgctggaa gtcgaacgcg
     5161 acgccgacgg ccgcctgctg gacgcccgcc gcaccggcga gcgcaatctg ccgctggaat
     5221 cgctgattca tctgcaaatc gaccgcatca gcgacgccgc ccagctggcg acggtggtgg
     5281 ccgaactgga gcaggtgctg aacgacatcc gcctggcggt ggaagacgag ccggccatgc
     5341 gccgcgagct gcaagacatt caagctgaac tgcaacaagc gccgctgccg gatggcaagc
     5401 tggacgtgaa cgagatttcc gcgtttctgg actgggtgaa cgcccgccac ttcttgctgc
     5461 tgggctactg cgcctacgac ctggtgcgca ccgacgacgg cgacgcgctg cgcattgtgc
     5521 cgggcagcgg ccacggcatt ttgcgcaatc agggcgacaa gacctattcg gccagctttg
     5581 ccgtgctgcc ggcgcacctg cgcgagctgg cttacgaccc ggcctgcccg atcatgctga
     5641 acaaatcgca gacccgctcg accatccacc gctcggcgca tctggacttt attggcatca
     5701 agcgctacga cgcccagggc catgtgattg gcgaatgccg ttttctgggc ctgtacaccg
     5761 ccgccgccta ccacgaatcg ccgcgcaata tcccgatttt gcgccgcaag atggacgcgg
     5821 tgatggcgga gtgtgattac gtcgaaaaca gctacaaaga aaaaaccctg caatttgtgc
     5881 tggaaagcta cccgcgcgac gagctgtttg aaatcccggt ggacgtcttg cagccgattg
     5941 ccgaaggcct ggtcaatctg ctggagcgcc cgcgcgtgcg cctgttcctg cgcaccgacc
     6001 tgtatcagcg ctatgtcagc gcgctggtgt ttgtgccgcg cgacagcttc agcaccgaag
     6061 tgcggctgaa aatcgaaaaa atcctgatgc aggcgctgaa cggcagcgcc gccgaataca
     6121 gcgtgtcgat ttctgacacc catctggccc gtgtgcacta catcatccgc acccctggcc
     6181 cgctgccggc gtttgacgcg ctgggcatcg agcaggacat cgcccgcatt gtgcgcggct
     6241 ggggcgacga gctgcgccag cagctgatcg acagccatgg cgaggcgcgc ggcaacacct
     6301 tgtattcgca ataccagcat gccttcccgg tggcctaccg cgaagacttt tcgccgcgcc
     6361 acgccgtgct ggacatcagc ctgatcgaag aagcgctggc tggcgcgccg ctggcgctca
     6421 agctgtacaa gccgctgcgc aagggcagcg ccgggcaaaa cctgaaagtg ttccgcgccg
     6481 gccagccggt cagcctgtcg gccagcctgc cggtgctgga gaatatgggc gtgcgcgtgc
     6541 aggacgaacg cccctacgcg gtggagcgcg ccgacggcgc cacggtgtgg atcaacgatt
     6601 ttggcctgga agtggccaat gtcgcccata tcgagcaaga cgatgtgcgc gagcgctttc
     6661 agcagctgct gcgccgcgtg gcggccggcc agtgcgagaa cgacggcttt aacaagctgg
     6721 cgctgctggc cgacctggac tggcgcgaag tgattctggt gcgcgcgctg gccaaatacc
     6781 tgcgccaggc cgggctgacc ttcagccaga gctatgtcga gcaatgcctg ggcaattacc
     6841 cggccatcac cggcggcctg gtgcgcctgt tcctggcccg cctcgacccg gcgcacgacg
     6901 acgccggcgc cagcgcccgg ctggaagccg aagtgcgcga gcaattcgag caagtcgcca
     6961 atctggacga agaccgcatc ctcaacggcc tgctgaccat catcctggcc atccgccgca
     7021 ccaactactg gcagcgcgac gccgccggcc agcccaagcc gtatctgtcg ttcaagctgg
     7081 aatccggggc gattcccttc ctgccgcaac cgcgcccgct gtttgaaatc tgggtgtacg
     7141 gcgcgcgggt ggaaggcgtg catctgcgcg gcagcaaggt ggcgcgcggc ggcctgcgct
     7201 ggtctgaccg catggaagac ttccgcaccg aagtgctggg cctggtgaag gcgcagatgg
     7261 tgaaaaactc ggtgattgtg ccgatgggct ccaagggcgg ttttgtctgc aagcaactgc
     7321 cgccggccag cgaacgcgaa gcctggctgg ccgaaggcgt ggcctgctac aagatgttta
     7381 tctccggcct gctcgacctg accgacaatc tggtcaacgg ccagatcgcc ccgccggtgg
     7441 cggtgcgccg cctggacgaa gacgacccct atctggtggt ggccgccgac aagggcacgg
     7501 cgacattctc cgacatcgcc aacagcgtgt ctgaagcata tggcttctgg ctgggcgacg
     7561 cctttgcctc gggcggctcg gccggctatg accacaaaaa aatgggcatc accgcgcgcg
     7621 gcgcctggga atcggtcaag cgccacttcc gccacctggg cgtcaacacc cagacgcagg
     7681 actttaccgt gatcggcatt ggcgacatgg ccggcgacgt gtttggcaac ggcatgctgc
     7741 tgtccgagca tatccgcctg ctggcggcgt tcaatcacca gcacatcttc cttgacccca
     7801 acccggacgc ggcggtgagc tttgccgagc gcgcgcggct gttcaacctg ccgcgctcca
     7861 gctgggccga ctacgacagc agcctgattt ctgacggcgg cggggtattc gagcgccgcg
     7921 ccaaatccat cccgctgtcg ccggccgtgc gcgcctggct gggcgtggag cgcgacagca
     7981 tggcgccgac cgagctgatt cacgccatcc tgcaagcgcc ggccgacctg ctgtacaacg
     8041 gcggcatcgg cacctatatc aaggccagca cgcaaagcca cgccgaagcc aacgaccgcg
     8101 ccaacgacgg cctgcgcgtc aacggcaatg agctgcgggt gaaggtgatt ggcgaaggcg
     8161 gcaacctggg catcacccag ctgggccgca tcgaatgcgc gcgccacggc gtggcgctgt
     8221 ccagcgacgc catcgacaac tcggccgggg tggattgctc tgaccacgaa gtcaacatca
     8281 aaatcctgct gggcggggtg atgcaggccg gcgacatgac gctcaagcag cgcaacgagc
     8341 tgctggcgga aatgaccgac gaagtcggcc atctggtgct gcgcaacaac tacctgcaaa
     8401 cccagattct ggcggtgaac aaagcccacg cggcgcaaat gctgtctgcc cagcagcgca
     8461 tgatcagcca tatggaaaaa accggcgagc tcaaccgcgc gctggaattc ttgcccgacg
     8521 acagcgccat cgccgaacgc cgcgccagcc gccagggcct gaccagcccg gaagtggccg
     8581 tgctgctggc ctacagcaag atttcgctgg accgcgcgct gcaagcctcg gccctgcccg
     8641 atcaccccga tctggacagc gtgctggtga actacttccc gtcggcgctg caaacccgct
     8701 ttgccgacgc catgcgccgc cacccgctca agcgcgaaat catcgccaac cagctggcca
     8761 accaagtggt caaccgcatg ggcaccacgt ttatcttccg catgcaggaa gaatccgcct
     8821 ggccggccgc cgacattgtg caagcctggg tgacagtcaa ccgcatcttt gacggcgaag
     8881 cgctgtggaa cgccatcgaa gcgctggaca accgcgtgcc ggccgatgtg caggtgagca
     8941 tgctggtgca agtgcgcacg ctgatcgagc gcgccacccg ctggctgctg cgcagcggcc
     9001 gcccgctggg cgacatcgcc gccgccgtgg cccgctgccg cgcgccgatg gccgaactgc
     9061 tggccggcct gcctggctgg atcgacgccg cccattaccc ggcggtggcc gactgggagc
     9121 aacgcctgca cgccgcccac accccggccg aactggccgg cgtggtggcc cgcctggact
     9181 ttgccgtggc cggctttgac atcctggaac tggcgcaagc gcaaaacctg ccgctggccg
     9241 tggtggcggc caactacttc gagctgggcc gcgtgctgga gctggactgg ctgcgcgacg
     9301 ccatcacccg cctgccgcgc gacaaccgct ggcaagccct cacccgctcg gcgctgcgcg
     9361 acgacctgta tcgcctgcac cgcgaactca ccgccagcgc cctggcctgc ccggcctgcg
     9421 gcgaagcgca gtacgccaag cactggctgg ccggcaaagg cggcgaactg gaagtctgcc
     9481 agcaaatgct gagcgaactg caaggctacg acacgctgga cctggccatg ctgtcggccg
     9541 gcatgcgcga agtgggcaac cacctgatgc gttgatggtc tatagtgaaa atctagaggg
     9601 caacccggat gtgaatccgg gtgaaggtga aaccgcgcgg gagaccgcgc ggtttttttg
     9661 tgttacgaat caatactggg ggttgcaact atcaacttga actttcttga tttccatata
     9721 ggtcagatgt acatttccac ctaatacgga taaaattgtg cggcaattta tatcattttt
     9781 aggcatttca aaccaatcag aaaatatcat tatgatttgc attaattgca cgacacacgc
     9841 cccgcttaaa aatttgatca tagaaaacaa tgaggttggg gtgtgcggtt actgtggtca
     9901 atttaatttg gcgattgaaa aagccacatt att
//
LOCUS       DAOPYX010000088         9852 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_8069_length_9852_cov_9.216903, whole genome shotgun sequence.
ACCESSION   DAOPYX010000088 DAOPYX010000000
VERSION     DAOPYX010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 9852)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9852)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9852
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8069_length_9852_cov_9.216903"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..323)
                     /locus_tag="PKN55_06120"
     CDS             complement(<1..323)
                     /locus_tag="PKN55_06120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011136309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HNX70850.1"
                     /translation="MHPYDLPDAQGHFGRYGGVYVAETLMSALDELTREYARYKNDPE
                     FLAEYQNELKHYVGRPSPIYHARRLSEACGGAQIYLKREDLNHTGAHKINNAIGQALL
                     AKRMG"
     gene            complement(310..933)
                     /locus_tag="PKN55_06125"
     CDS             complement(310..933)
                     /locus_tag="PKN55_06125"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014086702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HNX70851.1"
                     /translation="MPVRIKICGITRPEDGQAAAQLGADAIGLVFYPKSPRHVSLEQA
                     RAIVAALPPLVTVVALFVDPEPDEVHRVLAAVPIDVLQFHGEESPDFCRQFQRPYLKA
                     VRVQPGLDLLQYAIAHAGARGLLTDAFVPGVPGGTGATFDWSLLPADLPLPLILSGGL
                     EPDNITAAVRAVRPAAVDTSSGVEASKGIKDAARMAAFIAGVHHASV"
     gene            complement(973..1767)
                     /gene="truA"
                     /locus_tag="PKN55_06130"
     CDS             complement(973..1767)
                     /gene="truA"
                     /locus_tag="PKN55_06130"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018747965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HNX70852.1"
                     /translation="MRIALGVEYDGRGFSGWQTQPNGNTVQDALERAVAAIVGVPVRC
                     HAAGRTDAGVHATGQVAHLDCDVPRPPGAWVRGVNSHLPPGVAVCWAQPVPEHFHSRF
                     SAYARCYRYVLLNRPVRPAIAAGQVGWFHAPLEVEAMREASAHLLGQHDFSSFRAAEC
                     QAKTPVKTLTRLDIERVGDTLTFSLEADAFLHHMVRNLVGALLMVGKGGKPAEWMAEL
                     LAARSRASAPPTFMPDGLYLTGVRYPEEFGLPSPPPLYGPRRQADV"
     gene            complement(1779..2456)
                     /locus_tag="PKN55_06135"
     CDS             complement(1779..2456)
                     /locus_tag="PKN55_06135"
                     /inference="COORDINATES: protein motif:HMM:NF014420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbiQ family ECF transporter T component"
                     /protein_id="HNX70853.1"
                     /translation="MGFSVSSPDRPAGSGFFHPATPLAAWLMLAVMLPLWPWPLQLAA
                     AAACAGLAARRERAAPWLAARRVRWLLAALLAAMAWSTPGQALWPADWAPTREGVQEG
                     GLHALRLLALLWCMKALFASRSRQAWLLGLRTLCWPLTWLGLDINRAALRLWLTLEES
                     ERLTGQAPPRWRDLWQALSAAPTAASEHAQGDEGRALTLQLQALTARDLLAWLVLALA
                     LAASVFG"
     gene            complement(2463..>3507)
                     /locus_tag="PKN55_06140"
     CDS             complement(2463..>3507)
                     /locus_tag="PKN55_06140"
                     /inference="COORDINATES: protein motif:HMM:TIGR03504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FimV/HubP family polar landmark protein"
                     /protein_id="HNX70854.1"
                     /translation="APAKPAAPAAADSLSMDDVFDFGLDAPAEPAPAPAAAKPAAPAP
                     APAPIADPVADTLSQDDLDDLFAQPAPAPAAKPEPAPVNDVLSQDDLDNLFGETPAAA
                     EPAADDGELDIDALIAAEQAKQAEEEAAQAAAIKPAPSKPAIPSGDELSLDFDGIFED
                     TVPPLPALDPEALAAALANAPDMDDLPPPDDTVLEAPDMPLEIPDEGVLAAELAEPEL
                     PEGDNPLKDEMSEPSPLLTGEMANIDPDLAFDFDAVFQDAAPAAPKLDRSALAAALAS
                     APDLEELHQEDAVTADSGEAITPEQVDTKLDLARVYIDMGDEEGAREILVEVMAEGTD
                     EQRDTALKLMEKL"
     assembly_gap    3508..3607
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3608..5505)
                     /locus_tag="PKN55_06145"
     CDS             complement(<3608..5505)
                     /locus_tag="PKN55_06145"
                     /inference="COORDINATES: protein motif:HMM:TIGR03505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FimV/HubP family polar landmark protein"
                     /protein_id="HNX70855.1"
                     /translation="MTIRAKLTASALALALIGATYPAMAGGLGRMHVQSALGQPFRAE
                     IDITGVRSQELSQIRVNIASPAAFAEMQMDYPEVLQGVSVNVVRKPDGKMVARVQGAQ
                     PISDPFLFLLLELNDGGARSFKEFTALLEPSDKPTPAAPAVSTEQAKPAASAAPAPAA
                     PAATPAPAPATSKPADSKPAAKAAASKPDDDGPLPSDGRQHTVKPGETLSGVAAKLKP
                     ADVSLEQMMVGLYRNNSGAFAGDMNKLRAGATLDVPAADGVRSIPPRDALDTVRAQVK
                     DWRNYVLGGADAAAAAPARDPGKTRTPPSDASAEVAPPDNKDGVLKLSKADGKPGEER
                     PGQLEEELVSREKALKDANERITTLEAQLEEMRGLLALRSQMGAEAEKQAKEAEAGKA
                     EAPPRPASGAQASASEGQHNAMFLLGGLGLAGALLAGVLYAHRRKDTFRRWWEQLMAA
                     RRKKAEEKAAEKAAKADSLAASTEPAPAAAAADSNDEDPLVVADRLIEYNKLAQAETV
                     LLDALNKTPDRHELRLKLLKVYYAMPLVDKFNAQAARLLDAVSKTSPLWVQALEMGRK
                     LDPNHPLYAAPKPVEPAASDDFGFDEADIEPAPEPAPAPKPAAKPAPAPVAEDPFDLD
                     SDLLPDDAV"
     gene            complement(5662..6630)
                     /locus_tag="PKN55_06150"
     CDS             complement(5662..6630)
                     /locus_tag="PKN55_06150"
                     /inference="COORDINATES: protein motif:HMM:NF014793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asd/ArgC dimerization domain-containing protein"
                     /protein_id="HNX70856.1"
                     /translation="MNTPLDIAVVGATGALGRVLLEVLGDSRFPLGRLYPLASERAET
                     EGDTLTFRNRELTVGDAEGFDFSRAQLVLLAVPADCADSLAADACAAGAAVLDFSSAQ
                     RNNPALVLAGGQAEGPAPGQRVRCPDGLSLAVASLLEGLTPAGVESVDITALLPAGVH
                     GRAAMDELAGQTTALFNLSETTTQVFDRRLAFNLLPASLDEHQQRSQDVARLLGDAAP
                     TVALNLAVAPVFFGASLSLTLHGSALDEAAVRAAWAANTLIRLDDDSAVLSPADAATR
                     PDLAVAGLHRQGDALCAWAAFDPLRWVALHALRLSARTLASLQQPA"
     gene            7244..9286
                     /locus_tag="PKN55_06155"
     CDS             7244..9286
                     /locus_tag="PKN55_06155"
                     /inference="COORDINATES: protein motif:HMM:NF028510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cache 3/Cache 2 fusion domain-containing
                     protein"
                     /protein_id="HNX70857.1"
                     /translation="MRIKHLPLATAIAIMASLVITVIFSCLIGFLSWSSNRTAVKQVH
                     EHLSQQMSAIAIALEDNFLSAQQSAKLRFGMYKHMLPSQSFTVTQEGAPSEGLLAGVP
                     VIKAGELALNNNMAFLEKVKSMLDADPAVMVLHNGKFVRVATFLKKDGKSQIGVPLAD
                     DSKEAKTLHKGETFYGMVNRSGVFYMSIFEPVYDANKKLIGAISVRVSVDDIMARLRQ
                     SISNIKVAQTGYAYVMQPGKTVDDTIMLVHPSLAGKRVGDMKNEQITRVVGELIATRN
                     GVLHYRWDDAKSGVSGDKIAAVAEVKNTGWIVASGSWEDEFTAEARNLRNGLILAAAL
                     SGVLTVTLIAWAIRRGLAPVQTLVESVRQMGAGDFSRTLNASDEASRNEVDRLQNSLS
                     GTQRDIAGVLRRMADSARALGQVTSQLDQSSDKVMDSSGHQADAAATLARTAEEMANS
                     IGHVSDNASQAEQLAQHTADAAQAGSHKVNEVASEMHQIENEIMSAAQVVHTLGQRTS
                     EINQVIQLIKEVADQTNLLALNAAIEAARAGEQGRGFAVVADEVRKLAERTTQSTMEI
                     SSSIGSVQQESGEVVRRIEQVVERIAAGVQAVENAGQVLGEIEAQSKQAVTAVAGIAV
                     SMREQSGSSQSIADSVETIAAMSEANSQACRDNREHTRQLSQLASDLDGMVSRFKV"
     gene            complement(9403..>9852)
                     /locus_tag="PKN55_06160"
     CDS             complement(9403..>9852)
                     /locus_tag="PKN55_06160"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNX70858.1"
                     /translation="RAGEQGRGFAVVADEVRNLAERTSQATGEITRLIEAICQDTQGT
                     VDGMREVSQRMQDGLARLEDTQQVLAHIETTAEDTLEKATSISHAMREHSQASHDVAE
                     NVARIASHAQENSALIGEASRIAQTMNGTAQELNALVGRFVLPRNGA"
BASE COUNT         1652 a         3049 c         3287 g         1764 t
ORIGIN      
        1 ttgcccatgc gcttggccag cagcgcctgg ccgatggcgt tgttgatctt gtgcgcgccg
       61 gtgtggttga ggtcttcgcg cttgaggtaa atctgcgcgc cgccgcaggc ttcgctcagc
      121 cggcgggcgt ggtaaatcgg gctggggcgg ccgacgtagt gcttgagttc gttctggtat
      181 tcggccagaa actcggggtc gttcttgtag cgggcgtatt cacgcgtcaa ttcgtccagc
      241 gccgacatca gggtttcggc gacatacacg ccgccgtaac ggccaaagtg gccttgtgcg
      301 tcgggaaggt catacggatg catggtgaac tcctgcgata aatgccgcca tgcgggcagc
      361 gtccttgatg cctttgctgg cttccacgcc gctggatgtg tccacggcgg cgggacgcac
      421 ggcgcggacg gcggcggtga tgttgtcggg ctccagcccg cccgagagga tcagcggcag
      481 cggcaaatcc gctggcagca ggctccagtc aaaggtggcg ccggtcccgc ccggcacgcc
      541 cggcacaaag gcgtcggtca gcaaaccgcg cgccccggcg tgggcaatcg cgtattgtag
      601 caaatccagg ccgggctgga cgcgcacggc tttcagataa gggcgttgaa actgccggca
      661 aaaatccggg ctttcttcgc cgtgaaactg caagacgtca atcggcacgg cggccagcac
      721 gcggtgcacc tcgtccggct cgggatcgac aaacagcgcc accaccgtca ccagcggcgg
      781 caaggcggcg acaatggcgc gcgcttgttc caggctgaca tggcgcgggc ttttgggata
      841 aaaaaccagg ccgatggcgt cagcgcccag ctgggcggcg gcctggccgt cttcggggcg
      901 ggtgatgccg cagattttga tgcgaacggg catggtgaat gggctcggca aaaataacag
      961 ggtgaaaacc gctcaaacat ccgcctgtcg gcgcgggcca tacagcggcg gcggcgaggg
     1021 caggccgaat tcttccgggt agcgcacgcc ggtcaggtac aggccatcgg gcatgaaggt
     1081 gggcggcgcg ctggcgcggc tgcgcgccgc cagcagctcg gccatccact cggccggctt
     1141 gccgcccttg cccaccatca gcaaggcgcc aaccagattg cgcaccatat gatgcaaaaa
     1201 ggcgtcggct tccaggctga aggtgagggt gtcgcccacc cgctcgatat ccagccgggt
     1261 gagggttttc accggggtct tggcctggca ctcggcggcg cgaaagctgg aaaaatcatg
     1321 ctggcccaat aaatgcgcgc tggcctcacg catggcctcc acctccagcg gcgcatgaaa
     1381 ccagcccacc tggccggcgg caatcgccgg gcgcaccggg cggttcagca gcacatagcg
     1441 gtagcagcgg gcataggcgg aaaaacgcga atgaaaatgc tcgggcaccg gttgcgccca
     1501 gcacaccgcc acgccgggcg gcaaatggct gttcaccccg cgcacccagg cgccgggcgg
     1561 gcgcggcaca tcgcaatcca gatgcgccac ctggccggtg gcgtgcacgc cagcgtcggt
     1621 gcgcccggcg gcatggcagc gcaccggcac gccgacaatc gccgccactg cgcgctccag
     1681 cgcatcttgc acggtgttgc cgttgggctg ggtttgccag ccggaaaacc cgcgtccgtc
     1741 gtattcgacg cctaatgcaa ttctcatggg tgactgtgct atccgaaaac cgaagccgcc
     1801 agcgccaagg ccaggacaag ccaggccagc aggtcgcggg cggtcaaagc ctgcaattgt
     1861 agcgtcaatg cccgtccctc gtcgccctgt gcgtgctctg acgccgccgt tggcgcggcg
     1921 gacaaggctt gccacaggtc gcgccagcgc ggcggcgcct ggccggtcag ccgttcgctt
     1981 tcttccagcg tcagccacag ccgcagcgcc gcgcggttga tgtccagccc cagccaggtc
     2041 agcggccagc acagcgtgcg caggcccaac agccaggcct ggcggctgcg gctggcgaac
     2101 agcgccttca tgcaccacag caaggccagc agccgcagcg cgtgcaggcc gccttcctgc
     2161 acgccttcac gggtgggcgc ccagtccgcc ggccacagcg cctggccggg cgtgctccac
     2221 gccatcgccg ccagcagcgc cgccagcagc cagcgcaccc gccgcgccgc cagccacggc
     2281 gccgcccgct cgcgccgcgc cgccagaccg gcgcaagccg ccgcggcggc cagttgcagc
     2341 ggccacggcc acaagggcag catcaccgcc agcatcagcc aggcggccag cggggtggcg
     2401 ggatgaaaaa accccgagcc ggcgggccgg tcggggctgg aaacagaaaa acccatcacg
     2461 atttacagct tttccatcag cttcagcgcg gtgtcgcgtt gttcgtcggt gccttcggcc
     2521 atgacctcca ccaggatttc gcgggcgcct tcttcgtcgc ccatgtcgat gtagacgcgg
     2581 gcaagatcca gcttggtgtc cacttgttcc ggggtgatgg cttcgccgct gtcagcggtc
     2641 acggcgtctt cctggtgcaa ttcttccagg tccggggcgc tggccagggc ggcggccagc
     2701 gcgctacggt ccagcttcgg cgcggccggg gcggcgtcct ggaacacggc gtcaaagtca
     2761 aatgccaggt ccgggtcgat attggccatc tcgccggtca gcagcggcga gggctcgctc
     2821 atttcgtcct tgagcgggtt gtcgccttct ggcagttccg gctcggccag ttcggcggcc
     2881 agcacgcctt cgtcggggat ttccagcggc atatccggcg cttccagcac ggtgtcgtcc
     2941 ggcggcggca ggtcgtccat gtccggggcg ttggccaggg cggcggccag ggcttccggg
     3001 tcgagcgccg gcagcggcgg cacggtgtct tcgaagatgc cgtcaaagtc cagcgagagt
     3061 tcgtcgccgg atgggatggc cggtttgctc ggcgccggct tgatggcggc ggcctgggcg
     3121 gcttcttctt cggcttgttt ggcttgctcg gcggcaatca gcgcgtcgat gtccagctcg
     3181 ccgtcatccg ctgcgggttc tgccgcggcc ggggtttcgc caaacagatt gtccagatcg
     3241 tcctggctca gcacatcatt gaccggggcc ggctcgggct tggcggccgg ggcgggcgcc
     3301 ggttgggcaa agaggtcgtc caggtcatcc tggctgagcg tgtcggccac cgggtcggca
     3361 atcggcgccg gggcgggggc tggcgcggcc ggtttggcgg cagcaggggc cggcgccggt
     3421 tcggccgggg cgtccaggcc aaagtcgaac acatcgtcca ttgacaggct gtcggcggcg
     3481 gccggcgcgg cgggtttggc cggggctnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3601 nnnnnnnacg gcgtcgtcag gcagcaggtc gctgtccagg tcgaacggat cttctgccac
     3661 cggggcgggg gccggtttgg cggcgggttt gggcgctggg gcgggttctg gcgccggttc
     3721 gatgtcggct tcgtcaaagc caaagtcatc gctggctgcc ggttcaactg gcttgggcgc
     3781 ggcgtacagc gggtggttgg ggtcaagctt gcggcccatt tccagcgcct gcacccacag
     3841 cgggctggtc ttactcacag cgtccagcag gcgggcggcc tgggcgttga acttgtccac
     3901 cagcggcatg gcgtagtaca ccttgagcag cttcaggcgc agttcgtggc gatccggggt
     3961 tttgttcagc gcgtccagca gtacggtttc tgcctgagcc agcttgttgt attcgatcag
     4021 gcggtcggcc acgaccagtg gatcttcgtc gttgctgtca gccgcagccg cagcgggggc
     4081 gggctcggtg ctggcggcca gactgtcggc tttggcggct ttttctgctg ctttttcttc
     4141 ggcttttttg cgccgggccg ccatcaattg ctcccaccag cggcggaagg tgtctttgcg
     4201 acggtgcgcg tacagcacgc cggccagcag ggcgccggcc aggcccagcc cgcccagcag
     4261 gaacatggcg ttatgctggc cttcgctggc actggcttga gcgcctgatg ccggacgcgg
     4321 cggggcttca gccttgccgg cttcggcttc cttggcttgt ttttcggctt cggcgcccat
     4381 ttgcgagcgc aaggccagca ggccgcgcat ttcttccagc tgggcttcca gcgtggtgat
     4441 gcgttcgttg gcgtctttca gggctttttc gcggctgacc agttcttctt ccagttggcc
     4501 ggggcgttct tcgccgggtt tgccgtcggc cttgcttagc ttaagcacgc cgtctttatt
     4561 gtctgggggg gccacttcgg cgctggcgtc gctgggtggg gtgcgggttt tacctgggtc
     4621 gcgcgctggt gcggcggcgg cggcgtctgc gccacccagc acataattgc gccagtcttt
     4681 gacctgggcg cgcacggtgt ccagcgcgtc gcgcggcgga atgctgcgca cgccgtcggc
     4741 ggcgggcaca tccagggtgg cgccggcgcg cagtttgttc atgtcgccgg caaacgctcc
     4801 gctgttattg cggtacaggc cgaccatcat ttgttccagc gagacatctg ccggcttgag
     4861 cttggccgcg acgccagaca aggtttcgcc aggtttgacg gtgtgctggc ggccatcgct
     4921 gggcagcggg ccatcatcgt caggcttgct cgctgcggcc ttggccgccg gtttgctgtc
     4981 ggcaggcttg ctggttgcag gggccggtgc gggggttgct gccggcgcgg ctggcgcagg
     5041 cgctgcactg gcagccggct tggcttgttc tgtgctcacc gccggcgccg cgggcgtggg
     5101 cttgtcgctg ggctccagca gggcggtgaa ttccttgaac gagcgggctc cgccgtcgtt
     5161 cagttccagc agcaagaaca ggaaagggtc gctgatcggc tgggcgccct gtacgcgggc
     5221 taccatcttg ccatcgggtt tgcgcaccac gttgacgcta accccttgca ggacttcggg
     5281 gtaatccatc tgcatctcgg caaaagcggc cggtgaggcg atgttgacgc gaatctggct
     5341 gagttcttgc gagcgaaccc cggtgatgtc gatctcggcg cggaacggct gccccagcgc
     5401 ggattgcaca tgcatgcggc ccagcccgcc ggccatggcc ggataagtgg caccgataag
     5461 cgccagcgcc aaggcgctgg cagtgagttt cgctcgaatc gtcacgaagc ctgcccttac
     5521 gaatgtcagt aaagcccgtc gtcgctggcg cactgccagg acgatgtgtg cggatgatac
     5581 ccagcatggc ggcaaaatgc catgcagatc gttgcgtata acccgccagt gcgtgcgcat
     5641 ggcgggcaaa ataccggcga ttcaggccgg ttgttgcagg ctggccaggg tgcgtgccga
     5701 cagtcgcagc gcgtgcagcg ccacccagcg cagcggatca aacgccgccc aggcgcacag
     5761 cgcatcaccc tggcgatgca ggccagccac ggccagatcc gggcgggtgg cggcatcggc
     5821 cgggctgagc acggcgctgt catcgtccag gcggatcagg gtgttggctg cccaggccgc
     5881 gcgtacggcg gcttcatcca gcgcgctgcc gtgcaaggtc agcgacagac tggcgccaaa
     5941 aaacaccggg gccacggcca ggttcagcgc cacggtcggg gcggcatcgc ccagcaggcg
     6001 ggccacatcc tggctgcgct gctggtgttc gtccagcgag gccggcaaca ggttgaatgc
     6061 cagccggcgg tcaaacacct gggtggtggt ttcgctgagg ttgaacagcg cggtggtttg
     6121 cccggccagc tcgtccatgg ctgcgcggcc atgcacccct gccggcaaca atgccgtgat
     6181 atcgaccgat tccacgccgg ctggagtcag gccttccagc aggctggcca cggccaggct
     6241 caggccatcc ggacagcgca cgcgctgacc aggcgccggg ccttcggcct ggccgccagc
     6301 cagcaccagc gccgggttgt tgcgctgggc gctggaaaaa tccagcacgg ccgcccctgc
     6361 ggcgcaggcg tcggccgcca ggctgtcggc gcagtcggca ggcacggcca gcagcaccag
     6421 ttgcgcgcgg gaaaaatcaa aaccttcggc gtcgcctacc gtcagttcgc ggttgcggaa
     6481 ggtgagggtg tcaccttcgg tttcggcccg ctcgctggcc agtgggtaca gccggcccag
     6541 cggaaaacgg ctgtcaccga gcacctccag caaaacccga cccagggcgc cggttgcgcc
     6601 aaccacggcg atgtcaagag gtgtgttcat cggtcaaaat cctcgcgaaa ttcaaaaaac
     6661 aagagtcggc ccggtggggc ggcccgatca tgggcgattg ggcccatacc gccatacggg
     6721 ggccgggtgt ggcgattgta acaggcccgg cgtgtttcac gggcgcagaa gatggtggcg
     6781 atttgcgttg atccacccga catcaggctt gcttttgcgc aaaaaatgcc atatgatcta
     6841 tatgtttacg gttttatttg ctgtcctgct cgcaccagat tcgcgtttgg catgcgcaag
     6901 gcatgctgcc gacgccgatc atcatcctgc ccggcctgtg gccagccata ctgcgcgtgc
     6961 ctgtcacgcc tccctcgatc tggcgtcatc tgtcctgtca cgttgcactt tttgcccgat
     7021 gtctggccgg ttcatgacaa aagtgttgaa attcaataag aacaatcgtt tgcttttttc
     7081 tgaaaagcca acgcgccggg cattcgttct ataaaaaccg cttgtgtggg cgcaaaagaa
     7141 tttggtatca ctgcgcatgg gccgcccgcg cggggtgacg gcccggttat tcaaaaaaac
     7201 aagacgcggt gtatcgtcac acgcgtgaat gaggagattt gccttgcgaa tcaaacacct
     7261 gcccttggct accgccattg ccatcatggc gtcgctggtc atcaccgtga ttttttcctg
     7321 tctgatcggt tttctgtcct ggtcatccaa ccgcacggcg gtcaaacagg tgcatgagca
     7381 cttgtcacag cagatgagcg ccattgccat tgcattggaa gacaactttc tgtcggctca
     7441 gcaatcggcc aaactgcggt tcggcatgta caagcacatg ctgccctcgc aaagcttcac
     7501 tgtgacgcaa gagggggcgc ccagcgaagg gctgctggct ggcgtgccgg tgatcaaggc
     7561 tggcgagctg gcactgaaca acaatatggc ctttctggag aaggtcaaaa gcatgctgga
     7621 tgccgatccg gcggtgatgg tgctgcacaa tggcaaattt gtgcgggtgg ccacctttct
     7681 gaaaaaagac ggcaaatcac aaattggcgt gccgctggcc gacgacagca aagaagccaa
     7741 aaccctgcac aagggcgaaa ccttctatgg catggtcaac cgcagcggcg tgttctacat
     7801 gtcaattttc gagccggtgt acgacgccaa taaaaagctg attggcgcca tttcggtgcg
     7861 ggtcagcgtc gatgacatca tggcgcgcct gcgtcagagc atcagcaata tcaaggtggc
     7921 acaaaccggt tacgcctatg tgatgcagcc aggcaaaacg gtggacgaca ccatcatgct
     7981 ggtgcatccg tcactcgctg gcaagcgggt aggtgacatg aaaaacgaac agatcacccg
     8041 ggtggtgggc gagctgatcg ccacccgtaa cggtgtgctg cattaccgct gggatgacgc
     8101 caagagcggg gtgagcggcg acaagattgc cgcggtggcc gaagtcaaga acactggctg
     8161 gattgtcgcc tcgggcagtt gggaagacga attcaccgct gaggcgcgca atctgcgcaa
     8221 cggcctgatt ctggctgccg ccttgagtgg cgtgctgacg gtcacattga tcgcctgggc
     8281 cattcgccgt ggcctggcgc cggtgcaaac cctggtggaa agcgtgcggc aaatgggcgc
     8341 cggtgatttc agtcgcacgc tgaacgccag tgatgaggcc agccgtaatg aggtggaccg
     8401 cctgcaaaac agcctgagcg gcacccagcg cgacatcgct ggcgtgttgc gccgcatggc
     8461 cgattcggcg cgggccttgg gtcaagtcac cagccagctg gaccagagtt cggacaaggt
     8521 gatggacagc tcgggccatc aggctgacgc cgccgccacg ctggcgcgca ccgccgaaga
     8581 aatggccaac agcattggtc atgtcagcga caacgccagc caggccgagc aactggccca
     8641 gcacactgcc gacgcggccc aggccggcag ccacaaggtc aacgaagtgg ccagcgaaat
     8701 gcaccagatt gaaaacgaaa tcatgtctgc cgctcaggtg gtgcacaccc tgggtcagcg
     8761 caccagcgaa atcaatcagg tgattcagct gatcaaggaa gtcgccgacc agaccaacct
     8821 cctggccttg aacgccgcga ttgaagccgc ccgcgcgggc gagcaagggc gggggtttgc
     8881 cgtggtggct gacgaagtgc gcaagctggc cgagcgcacc acgcaatcca ccatggaaat
     8941 ctccagttcg attggctcgg tgcagcaaga atctggtgaa gtggtgcgcc gcatcgaaca
     9001 ggtggtcgag cgcatcgccg ccggcgtgca ggcggtggaa aacgccggtc aggtgctggg
     9061 cgagatcgaa gcgcaaagca agcaggcggt caccgccgtg gcgggtatcg ccgtcagcat
     9121 gcgcgagcaa agcggctcca gccaaagcat tgccgacagt gtggaaacca tcgccgccat
     9181 gtcggaagcc aatagccagg cctgccgtga caaccgcgag cacacccgtc agctgagcca
     9241 gctggccagc gatctggatg gtatggtgtc gcgcttcaag gtgtaagtct ggcagccact
     9301 gcacgccggg gctggcctgc cggtgagtga ccccatcgcc cagaaaaaac cgccgccggt
     9361 tgcaaaaccg gcggcggttt tttttagcgt attacagcaa gctcaggcgc cgttgcgtgg
     9421 cagcacaaag cgtccgacca gggcgttaag ctcctgggcc gtgccgttca tggtttgcgc
     9481 aatgcgcgag gcttcgccga tcagcgcgct gttttcctgg gcgtggctgg caatgcgtgc
     9541 cacattctcg gccacatcat ggctggcctg gctatgttcg cgcatggcat ggctgattga
     9601 agtggctttt tccagcgtat cttcagcggt ggtttcgatg tgcgccagca cctgctgggt
     9661 gtcttccagc cgggccaggc catcctgcat gcgctggctg acttcgcgca tgccatccac
     9721 cgtgccctgg gtatcctggc agatggcttc gatcaggcgg gtgatctccc cggtggcctg
     9781 gctggtgcgt tcagccagat tgcgcacctc atcggccacc acggcaaagc cacggccctg
     9841 ctcaccggct cg
//
LOCUS       DAOPYX010000089         9844 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_8083_length_9844_cov_8.840127, whole genome shotgun sequence.
ACCESSION   DAOPYX010000089 DAOPYX010000000
VERSION     DAOPYX010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 9844)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9844)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9844
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8083_length_9844_cov_8.840127"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..980)
                     /locus_tag="PKN55_06165"
     CDS             complement(<1..980)
                     /locus_tag="PKN55_06165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003746961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HNX70859.1"
                     /translation="MFNKISKTFQYGRHTVTLETGEIARQASGAVMVSMDDTVVLVTV
                     VGAQGVKPGQDFFPLTVDYQERTYAAGKIPGGFFKREGRPSEKEILTSRLIDRPIRPL
                     FPEGFYNEVQIVATVVSLNPEVDADIPAMLGASAALALSGLPFQGPIGAARVGYANGE
                     YILNPTKTELQTSELDLVVAGTQRAVLMVESEARELAETVMLGAVVFGHEQMQAAINA
                     INALADEVNPVLWDWAEPVRNEALIAQVREIAGADIKAAFAIRQKQVRSQKLNEAWDK
                     VKAALITEDTDTLTANEIKGIFKNLEAEIVRGQILAGEPRIDGRDTRTVRP"
     gene            complement(1189..1458)
                     /gene="rpsO"
                     /locus_tag="PKN55_06170"
     CDS             complement(1189..1458)
                     /gene="rpsO"
                     /locus_tag="PKN55_06170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HNX70860.1"
                     /translation="MAVSTEQKAGIVQDFQRAAGDTGSPEVQVALLTARINDLTPHFK
                     ANTKDHHSRRGLLKMVSRRRKLLDYLKKKDADAYRTLITRLGLRK"
     assembly_gap    1669..1768
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1933..2862)
                     /gene="truB"
                     /locus_tag="PKN55_06175"
     CDS             complement(1933..2862)
                     /gene="truB"
                     /locus_tag="PKN55_06175"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012697396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HNX70861.1"
                     /translation="MTVSRLPKPPKRPIHGVLLLDKPLGMSSNKALQTTRWHYHAEKA
                     GHTGVLDPLATGLLPVCLGEATKFSAHLLDADKGYTATVRFGHYSSTGDLEGELTPGE
                     RPIDFDQAGLLAAMASLTGPIEQVPPMYSALKHQGRSLYEYARQGVEIERKPRQVVIH
                     RLELLSFDGQDAVLDVQCSKGTYVRTLAADLGAKLGCGAHLAGLRRTRTAGFDIADAI
                     ALDALVEMTPEARMAMLLPVDILVNHLPIHTLNDAETHKICHGQPVHVAEKDAIIQGC
                     RLYDSQQRFVGIGRMRADGLLWPDRLLAFSPAA"
     gene            complement(2895..3308)
                     /gene="rbfA"
                     /locus_tag="PKN55_06180"
     CDS             complement(2895..3308)
                     /gene="rbfA"
                     /locus_tag="PKN55_06180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012697397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HNX70862.1"
                     /translation="MAKKQFSRADRISEQMQRELADIIRTELKDPRVGWVTVTGVDVT
                     RDYSHAKVFYTLMTSSDLDTTQHALERAAGFMRSEVARRIKLFTVPQLHFVYDHSIER
                     GVHMTQLINQVAQDDAASRVDDDAADSAADDNAKR"
     gene            complement(3393..6488)
                     /gene="infB"
                     /locus_tag="PKN55_06185"
     CDS             complement(3393..6488)
                     /gene="infB"
                     /locus_tag="PKN55_06185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HNX70863.1"
                     /translation="MLETNVKQFASELGLQPDHLLEQLRAAGVGKSSMEDVLTEQDKT
                     QLRNHLRRAHGGTVDNRVTLKRKETSEIRKADSTGKSRTIQVEVRKKRVVVLPEEEAA
                     KNAPAVAVPAAVTAEPAPVNTPAAEPAAPVQAEVPVAAPTATAAPVVETVTPTPAPTV
                     EPASPAAAPAIEAKEVKSEAKPEAKPEAKPAEARPASQPQAARPSNPAQKPAGQGRRD
                     DGRRDNRDNRGRDRDNRGPRNSAPQTPRLSPAAAAAAQMNAPQASAPVNPALILTPEE
                     IAARENEARRQDKLRAAQEALIRERQEREARRLVARQQAEADAAAAKQAASKPAAPAA
                     AAGEKTPKPAHGSVLHKPAKPAGAAGAARPAPAAAAGAPGAAKPAGGNDAPGRGGNKK
                     AGGKDSWDDGAKKRGGLKTKGSSDSRNDWKSKKGGKGNKHAQGAHQFQAPTEPIVHEV
                     LVPETISVAELAHKMAVKAAEVIKTLMKMGMMVTINQVLDQETAMIVVEEMGHLAKPA
                     KLDDPESYLDDVGGEHHEAVLESRAPVVTVMGHVDHGKTSLLDYIRRAKVAAGEAGGI
                     TQHIGAYHVDTPRGMVTFLDTPGHEAFTAMRARGAKATDIVVLVVAADDGVMPQTIEA
                     IHHAKAAGVPMVVAVNKIDKQGANPERIRQELSAHGVAPEDWGGDTQFVEVSAKQGTN
                     IDGLLEAILLQAEVLELQAPREAPAKGIVVEARLDKGRGAVATILVQSGTLKKGDVLL
                     AGTVFGRVRAMNDENGKPISEAGPSIPVEILGLSEVPAAGEDVMVLTDERKAREIALF
                     RQGKFRDVRLAKQQAAKLENMFAQMAEGEVQTLSVIIKADVQGSCEALAGSLQKLSTD
                     EVRVNILHSAVGGITESDVNLAAASSAIVIGFNTRADAQARKQAEANDVEIRYYNIIY
                     DAIDEVKAALSGMLAPERKEQVIGTVEIRQVFIVSKVGAIAGCMVTDGMVKRGSSVRI
                     IRQNVVVHQGELESLKRFKDDVKEVKQGYECGLQIKNFNDIQEGDQLEVFEIVEVARS
                     L"
     gene            complement(6572..8053)
                     /gene="nusA"
                     /locus_tag="PKN55_06190"
     CDS             complement(6572..8053)
                     /gene="nusA"
                     /locus_tag="PKN55_06190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012697399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HNX70864.1"
                     /translation="MSREILLLVDALACEKNVSKEIVFGALELALASATKKKSNQEDI
                     DVRVEIDRNTGDYHSFRRWQLVEDNDHEYPSRQVAVSDLDERHAGLELGDFYEEPLEA
                     IEFGRIGAQTAKQVILQKIRDAEREQVLNDFLERNDFLVRGNIKRMERGNAIIEIGKL
                     EAVLPREQMIPKENLRVGDPVRAYLLRVDRGGRGPQLVLSRTSAEFVKKLFELEVPEI
                     EEGLLEIRGAARDPGSRAKISVKANDPRIDPQGTCIGMRGSRVQAVTSELAGERVDIV
                     LWSPEQATYVINALSPAEVQRIMVDEEKHSMDVVVEEDQLALAIGRGGQNVRLASELT
                     GWILNIMTVDEAAEKHEAEDAALRSLFINSLDVDEEMAEVLISEGFTTLEEVAYVPLA
                     EMLEIDALDEETVNELRSRARNALLTQAIASEEKVESVSEELKTLEGIDSDLVRQLAE
                     HAITTRDDLADLAVDDLVEMTGMEADAATALIMKAREHWFSEE"
     gene            complement(8183..8608)
                     /gene="rimP"
                     /locus_tag="PKN55_06195"
     CDS             complement(8183..8608)
                     /gene="rimP"
                     /locus_tag="PKN55_06195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008954489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HNX70865.1"
                     /translation="MDVRALLESTLVGLGYEFVDLELTRSGGFRLFIDKPEGITVDDC
                     VLVSNHLTRLFMVENIDYDRLEVSSPGLDRPLKKEQDFVRFTGQLAKVKTRMPLEGRK
                     NFHGRLCGVEESALRLEVEGKLVSIPLASIDKARLDPEF"
     gene            9124..>9844
                     /locus_tag="PKN55_06200"
     CDS             9124..>9844
                     /locus_tag="PKN55_06200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014087855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNX70866.1"
                     /translation="MDKVWLKNYQPGVPAEIDINQFQSVSEVFARSVERFRDRPAMAN
                     MGKVYTYGELDALTVRFASFLQNTLKLPMGARVAVMMPNLLQYPVAVFAILRAGYTVV
                     NVNPLYTPRELEHQLNDAGVDTIVILENFANVLQQVIARTAVKHVVLTRIGDLLGAPK
                     SWLVNFAVKHVKKMVPAWKLPVTPVLFNDALKAGAAQPLTPVSIGHDDVAFLQYTGGT
                     TGVAKGAMLLHRNIVANMLQAG"
BASE COUNT         1616 a         3010 c         3014 g         2104 t
ORIGIN      
        1 gggcgcacgg tgcgggtgtc gcggccgtcg atgcgcggct cgccggccag gatctggcca
       61 cggacgattt cggcttccag gttcttgaag atgcccttga tctcgttggc ggtcagggtg
      121 tcggtgtctt cggtgatcaa cgcggccttg accttgtccc aggcttcgtt cagcttttgg
      181 ctgcgtactt gcttctggcg gatggcgaag gcggccttga tatcggcgcc ggcgatctcg
      241 cgcacttggg cgatcagcgc ttcgttgcgc accggctcgg cccagtccca cagcaccggg
      301 ttgacttcgt cagccagggc gttgatggcg ttgatggcgg cttgcatttg ctcgtggcca
      361 aacaccacgg cgcccagcat cacggtttcg gccagttcgc gggcttcgga ttccaccatc
      421 agcacggcgc gctgggtgcc ggccaccacc agatcgagtt cgctggtttg cagctcggtc
      481 ttggtcgggt tcaggatgta ttcgccattg gcgtagccca cgcgggcggc gccaatcggg
      541 ccctggaacg gcaggccgga caaggccagc gcggcggagg cgcccagcat ggccgggatg
      601 tcagcgtcca cttccgggtt cagcgacacc acggtggcga cgatttgcac ttcgttgtag
      661 aagccttccg gaaacagcgg gcggatcggg cggtcaatca ggcggctggt gaggatttct
      721 ttctcggacg ggcggccttc gcgcttgaag aaaccaccgg ggattttgcc ggcggcgtag
      781 gtgcgttcct ggtagtccac ggtcagcggg aagaaatcct ggcccggctt gacgccctgc
      841 gcgcccacca cggtgaccag caccacggtg tcgtccatgg acaccatcac ggcgccggag
      901 gcctgacggg cgatttcgcc ggtttcaagg gtgacggtgt gacgaccgta ctggaaagtc
      961 ttgctgattt tattgaacac gatgtgtgac tcttggtgag gattgccgga cacccggcaa
     1021 aacaacggga acccctagct attggggccg caagcgacgc caatgcctag aggctccccc
     1081 cgttgcgaca acagacgcgg caggcgccgc gccatgcgag acatatgcgc cccgcaaaag
     1141 ccaaaaaagt cgcagcaggc gctgcgactt tctggcaaaa ccacgtaatt acttacgcag
     1201 acccagacgg gtgatcaggg tgcgatacgc gtcagcgtct tttttcttca ggtagtccag
     1261 cagcttgcgg cggcggctca ccatcttcag cagaccacga cggctgtggt ggtctttggt
     1321 gttggccttg aagtgcgggg tcagatcgtt gatgcgtgcg gtcagcaggg ccacttgcac
     1381 ttcaggggaa cccgtgtcgc cagcggcgcg ttggaaatct tgaacgatac ctgctttttg
     1441 ttcggtagaa acagccatga aacactccag ataaaatatt gggcttgcgc ccggtcaaga
     1501 cgcggcgagc ctagacccct ccgcgctccc tgcctttgct tgagagtcgc tcacaaaacc
     1561 cgcctggcgt tgttgtgtga ccttgccgta ctcattgtac tgtctgcggt cacacgccta
     1621 gccaggcccg ctgcgctgag ttttgtgagc gactccgaga gccgctcann nnnnnnnnnn
     1681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnac gcctagccag gcccgctgcg ctgagttttg
     1801 tgagcgactc cgagagccgc tcacaaacct gcttggcgtt gtggtgtgac cttgctgtgc
     1861 tatttgcact gtctgcggtc acatgcctag ccaggatcgc tttgctgagt tttgtgagcg
     1921 actcttgtcc gctcaggctg ctggcgagaa cgccagcaat cggtccggcc acaacaggcc
     1981 atcggcccgc atgcgaccga ttccgacaaa ccgttgttgg gaatcgtata aacggcagcc
     2041 ttggattatc gcatcttttt cggcgacatg caccggctgg ccatgacaaa ttttgtgcgt
     2101 ttccgcgtcg ttcagggtgt ggatgggtag gtggttcacc aggatatcca cgggcagcag
     2161 catggccatg cgcgcctcgg gcgtcatctc caccagcgca tccagcgcga tggcgtcggc
     2221 gatgtcaaag ccggcggtgc gggtgcggcg caggccggcc aggtgtgcgc cacagcccag
     2281 cttggccccc aggtcggcgg ccagtgtgcg cacatacgtg cccttgctgc attgcacgtc
     2341 cagcaccgcg tcttggccgt caaagctgag cagctccagc cgatgaatca ccacttgccg
     2401 tggcttgcgc tcgatttcta ccccttgccg ggcgtattcg tacagcgagc ggccttggtg
     2461 cttgagcgcg gaatacatcg gcggcacttg ctcgatcggc ccggtcaggc tggccatggc
     2521 cgccagcagg ccggcctggt caaaatcaat cggccgctcg ccgggggtca gctcgccttc
     2581 caggtcgccg gtgctggaat agtggccaaa tcgcacggtg gcggtgtaac ccttgtcggc
     2641 gtccagcagg tgggcagaaa acttggtggc ttcgcccagg cacaccggca acaggccggt
     2701 ggccagtggg tcgagcacgc cggtgtggcc ggctttttcg gcgtggtagt gccagcgcgt
     2761 ggtttgcagc gccttgttgc tggacatgcc cagcggcttg tccagcaaca gcacgccatg
     2821 aatcggccgc ttgggcggct tgggcagtct gcttacagtc attgaagtca tcacatcgaa
     2881 cgcgggtagc tgcgtcagcg tttggcgtta tcgtcagcag cgctgtcggc ggcgtcatcg
     2941 tcaactcggc tggcggcgtc gtcctgcgct acctgattga tcagctgggt catgtgcacg
     3001 ccgcgctcga tcgagtggtc gtacacaaag tgcagttgcg gcacggtgaa cagcttgatg
     3061 cggcgcgcca cttccgagcg catgaagcct gcggcgcgct ccagcgcgtg ttgcgtggtg
     3121 tccagatcgc tgctggtcat cagcgtgtaa aacaccttgg cgtgcgaata gtcgcgggtg
     3181 acgtccacgc cagtgacggt gacccagccc acgcgcgggt ctttcaactc ggtgcggatg
     3241 atgtcggcca gttcgcgctg catctgctcg gagatgcggt cagcgcggga aaactgtttt
     3301 ttcgccatgg ggtctctcct gatggccgag acgggccggc catgaaaaaa ggggcgggct
     3361 tcgccgcccg cccccggtcg atctggctgc gcttacagcg agcgggccac ttcgacgatt
     3421 tcgaacactt ccagttggtc gccttcctgg atgtcgttga agttcttgat ttgcaggccg
     3481 cattcgtagc cttgcttgac ttccttgacg tcgtccttga agcgcttgag cgattccagt
     3541 tcgccctgat gcaccaccac gttctggcgg atgatgcgca ccgaggagcc gcgcttgacc
     3601 atcccgtcgg tgaccataca accggcaatc gcgccaacct tggacacaat aaacacctga
     3661 cggatttcca ccgtgccgat gacttgttcc ttgcgctccg gcgccagcat gcccgacagc
     3721 gctgccttca cttcatcaat ggcgtcatag atgatgttgt agtagcggat ttccacgtcg
     3781 ttggcttcag cctgcttgcg cgcttgcgcg tcggcgcggg tgttaaagcc aatgacgatg
     3841 gcactggaag ctgctgccag gttgacgtcg gattcggtga taccgcccac ggccgagtgc
     3901 aggatgttga cgcgcacttc gtcggtggac agtttttgca gcgaaccggc cagcgcttcg
     3961 caagaaccct gcacgtcggc cttgatgatg accgacaggg tttgcacttc gccttcggcc
     4021 atctgggcaa acatgttttc cagcttggcg gcttgttgct tggccagacg cacgtcacgg
     4081 aacttgcctt gacggaacag ggcgatttca cgcgccttgc gctcgtcggt cagcaccatc
     4141 acgtcttcac cggctgccgg cacttccgac agaccgagga tttccaccgg gatagacggg
     4201 cccgcttcgg aaatcggctt gccgttttcg tcgttcatgg cgcgcacgcg gccaaacacg
     4261 gtgcctgcca gcagcacatc gcccttcttc agggtgccgg attgcaccag gatggtggcc
     4321 acagcgccac ggcccttgtc caggcgggct tccaccacga tacccttggc cggggcttcg
     4381 cgcggggctt gcagttccag cacttcggct tgcagcagaa tggcttccag caggccatcg
     4441 atattggtgc cttgcttggc ggacacttca acaaactggg tatctccacc ccaatcttcc
     4501 ggcgccacac cgtgagcgga cagctcctgg cggatgcgct cggggttggc gccctgtttg
     4561 tcgatcttgt tgactgccac caccatcggc acacctgcgg ccttggcgtg gtgaatagct
     4621 tcgatggttt gcggcatcac gccgtcgtca gccgccacca ccagcaccac aatatcggtg
     4681 gctttggcgc cacgggcacg catggcggta aacgcttcgt gacccggggt atccaggaag
     4741 gtcaccatgc cgcgcggggt atccacgtgg taggcgccga tgtgctgggt aatgccgcca
     4801 gcttcgcccg ccgccacttt ggcgcgacgg atgtaatcca gcagcgaggt tttaccgtgg
     4861 tcaacgtgac ccatcaccgt caccaccggc gcgcgcgatt ccagcacggc ctcgtggtgt
     4921 tcgccgccga cgtcgtccag ataggattcc ggatcgtcca gcttggccgg cttggccagg
     4981 tggcccattt cttccaccac gatcatggcg gtttcctggt ccagcacctg gttgatggtg
     5041 accatcatgc ccatcttcat cagggtcttg atcacctcag cggccttcac cgccatcttg
     5101 tgggccagtt cggccaccga gatggtttcc ggcaccagca cttcgtgcac gatcggctcg
     5161 gtcggcgcct ggaactgatg ggcaccctga gcgtgcttgt tgcccttgcc gcccttcttg
     5221 gatttccagt cgttgcggct gtcgctgctg cccttggttt tcaggccgcc gcgcttcttg
     5281 gcgccatcgt cccagctgtc cttgccgccg gctttcttgt tgccaccacg gcccggagcg
     5341 tcgttgccac cggccggttt ggcggcgccc ggcgcacctg ctgcagcggc tggcgccgga
     5401 cgtgcagcgc ctgcggcgcc tgccggtttg gccggtttat gcagcacgct gccgtgggcc
     5461 ggtttcgggg ttttctcacc tgcggcagct gccggggcgg ccggcttgct ggcagcctgt
     5521 ttggcagcag ctgcgtcggc ttcggcttgc tggcgggcga ccagacggcg cgcttcgcgt
     5581 tcctgacgtt cgcgaatcag cgcttcctgg gcggcgcgca gcttgtcctg gcgacgagct
     5641 tcgttctcac gggcagcaat ttcttccggg gtgagaatca gcgccggatt gaccggtgcg
     5701 ctggcttgcg gcgcattcat ctgggcggca gcagcagcgg ccggcgacag gcgcggggtt
     5761 tgcggcgcgc tgttgcgcgg gccgcgattg tcacggtcgc ggccacggtt gtcgcggttg
     5821 tcacgacggc cgtcatcacg acgaccctga ccagcaggtt tttgcgccgg gttgctcggg
     5881 cgggcagcct ggggttgact ggccggacgg gcctcggcgg gtttggcttc cggtttcgct
     5941 tccggtttgg cttcggattt cacttctttg gcttcaatgg ccggggcagc agcagggctg
     6001 gccggctcga cagtgggcgc cggggtcggc gtcacggttt cgacaacagg cgcggcggtg
     6061 gcggtcggtg cggcgaccgg gacttcggcc tggaccgggg ccgccggttc ggcggcaggg
     6121 gtgttcaccg gggccggctc ggcggtcacg gcggcgggta cggcaacggc aggggcgttc
     6181 ttggcagctt cttcttccgg caacaccacc acgcgctttt tgcgcacctc cacttgaatc
     6241 gtgcgcgatt tgccagtgct gtcggccttg cgaatctcgg aggtttcctt gcgcttgagg
     6301 gtgacgcggt tgtctacggt gccgccatgg gcgcgacgca gatggttgcg cagctgggtt
     6361 ttgtcctgtt cggtcagcac gtcttccatc gaagacttgc ccacgccggc cgcgcgcagc
     6421 tgttcaagga ggtgatccgg ttgcaagcct aattcactgg caaattgttt tacattcgtt
     6481 tcaagcattc gcgttcctaa atatccctat cttttcgcct ggcccggccg cagggcaggc
     6541 gtaccgcccg ccgtcgttca catggccatc atcattcttc actaaaccag tgctcacgcg
     6601 ctttcatgat cagcgcagtg gcagcatcgg cttccatgcc ggtcatctcg accagatcgt
     6661 ccacagccag gtcggccaag tcgtcgcggg tggtgatggc gtgttcggcc aactgacgca
     6721 ccaggtcgct atcaatgcct tccagggtct tgagctcttc agagacgctc tcgacttttt
     6781 cttccgaagc aatggcctgg gtcagcaggg cattgcgtgc gcggctgcgc agttcattga
     6841 cggtttcttc gtccagcgcg tcaatttcca gcatctcggc cagcggcaca taggccactt
     6901 cttccagcgt ggtgaagcct tcgctgatca gcacttcggc catttcttcg tccacgtcca
     6961 gcgagttgat gaacaggctg cgcagcgcgg cgtcttcggc ttcgtgtttc tcggcggctt
     7021 cgtcaacggt catgatgttg agaatccagc cggtcagctc ggaggccagg cgcacgttct
     7081 ggccgccgcg gccaatcgcc agcgccagtt ggtcttcttc gaccaccacg tccatgctgt
     7141 gcttttcttc atcgaccatg atgcgctgca cttcggccgg cgacagcgcg ttgatcacat
     7201 aggtggcttg ttccggcgac cacagcacga tgtccacgcg ctcgccggcc agctcgctgg
     7261 tgaccgcctg cacgcgcgag ccgcgcatgc cgatgcaggt gccttgcggg tcgatgcgcg
     7321 ggtcgttggc tttcaccgaa atcttggcgc ggctgcccgg gtcgcgcgcg gcgccacgga
     7381 tttccagcag gccttcttcg atttccggga cttccagttc gaacagtttc ttgacgaact
     7441 cggccgaggt gcgcgacagc accagctgcg ggccacgacc accgcgatcc actcgcagca
     7501 gataggcgcg caccgggtcg cccacgcgca ggttttcctt ggggatcatc tgttcgcgcg
     7561 gcagcacggc ttccagcttg ccgatttcga tgatggcgtt gccgcgctcc atgcgcttga
     7621 tattgccgcg caccaggaag tcattgcgtt ccaggaagtc gttcagcacc tgttcgcgtt
     7681 cggcgtcgcg gattttttgc aaaatcacct gcttggcggt ttgtgcgcca atgcggccaa
     7741 attcgatggc ttccagcggt tcttcgtaga agtcgccaag ctccaggcca gcatgacgct
     7801 cgtccaggtc gctgactgcc acctggcgcg acgggtattc gtggtcgtta tcttcgacaa
     7861 gctgccagcg acggaaggaa tgatagtcgc cagtgttgcg gtcgatctcg acgcgcacgt
     7921 caatgtcttc ctgattgctt tttttcttgg tggcggaggc cagcgccagc tccagcgcgc
     7981 caaacacgat ttccttgctg acgttctttt cacacgccag cgcatccacc agcagcaaaa
     8041 tctctcgact catctaaaca ttctctccgc atcggccggc gatgtgttcg ccaaccctga
     8101 cgccggatgg cgcggggccc atcggcttgt gtgattgtct tcctcggcgc cgccgcctgc
     8161 cgggcggcgc attcaacctg gcttagaact cggggtcgag tcgtgccttg tcgatgctgg
     8221 ccagcggaat gcttaccagt ttgccttcca cttccaggcg cagggcgctt tcttccacgc
     8281 cgcacaggcg gccgtggaaa ttcttgcggc cttccagcgg catgcgggtt ttcaccttgg
     8341 ccaactgacc ggtgaagcgg acgaagtcct gttctttttt cagcgggcga tccaggcccg
     8401 gcgacgacac ttccaatcgg tcgtagtcaa tgttttccac catgaacagg cgggtcaggt
     8461 gattgctgac cagcacgcag tcatccacgg taatgccttc cggcttgtcg atgaacagac
     8521 gaaaaccgcc cgatcgggtc aattcgaggt cgacaaattc gtacccgagc ccgacgagcg
     8581 tgctttccag caacgcgcga acatccatgt tttttccaca aataaaaaat gggcgaaacg
     8641 cccacactga ttccattgat tataacagac tcttgaaaga aaaagaaact gctggtcatc
     8701 accgaccaag tgtggcagaa aatgtggcct ccgacaagta tctgaacttg acgaaagcat
     8761 gagtggtgcc gcagcctgca agtgggattt agcgccgcta acaaaaccct cctggcgtcg
     8821 ttgcgcagcc gtatttcttg tactgcccgc cgccgcacgc ttgaccggga acgcggtgct
     8881 caggctttgt acgcgactct taacgcgaca gggtggacag aagcccagcg gtcatgccat
     8941 atttcaaaca atcgtttgta acacggctcg tgccggacaa cgatgcggca tctatagtaa
     9001 aagtcacctc gcctgccctg caagcgccag cgagcgacgc ccgccaaagc atactgaccg
     9061 acctgctgct cgccgcacac agccaggacg gcgccacctc aaagacgatg gagaaacaag
     9121 acaatggaca aagtctggct gaagaattat caacccggcg tgcccgccga aatcgacatc
     9181 aaccagttcc agtcggtttc ggaagtgttt gcccgcagtg tcgagcgttt tcgagaccgg
     9241 ccggcgatgg ccaatatggg caaagtgtac acctatggcg agctggacgc cctgactgtt
     9301 cgttttgcct cgttcctgca aaataccctc aagctgccga tgggcgcgcg ggtggcggtg
     9361 atgatgccca atctgctgca atacccggtg gcggtgttcg ccatcctgcg cgccggctac
     9421 accgtggtca atgtcaatcc cttgtatacc ccgcgtgagc tggagcatca actgaatgat
     9481 gccggggtgg ataccatcgt tatcctggaa aattttgcca acgtgctgca acaggtgatc
     9541 gcccgtacgg cggtcaagca cgtggtgctg acccgcattg gcgacttgct gggcgcgccc
     9601 aagtcctggc tggtgaactt tgccgtcaag cacgtcaaga agatggtgcc agcctggaag
     9661 ctgccggtga cgccggtgtt gttcaacgat gcgctcaagg ctggcgccgc gcagccgctc
     9721 acaccggtga gcattggcca tgatgatgtg gcgtttttgc aatacaccgg cggcaccacc
     9781 ggcgtggcca agggcgccat gctgctgcac cgcaatattg tcgccaatat gctacaggct
     9841 ggcg
//
LOCUS       DAOPYX010000090         9795 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_8132_length_9795_cov_11.083162, whole genome shotgun sequence.
ACCESSION   DAOPYX010000090 DAOPYX010000000
VERSION     DAOPYX010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 9795)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9795)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9795
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8132_length_9795_cov_11.083162"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..261
                     /locus_tag="PKN55_06205"
     CDS             <1..261
                     /locus_tag="PKN55_06205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNX70867.1"
                     /translation="VDRADHADHAIREIGEASANTVTMVNEISAAIREQSQASSSIAE
                     QVERIARMAQTASGAAGNAAQAADSLEKLASQQRDTLGHYTV"
     gene            316..1062
                     /locus_tag="PKN55_06210"
     CDS             316..1062
                     /locus_tag="PKN55_06210"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HNX70868.1"
                     /translation="MLLALALFAAALLAGVVNALAGGGLLFTFPALVALGVPPVAANA
                     TANIASWPGYASAAFAMRRELAHAGGLRLLLISGVAGGVTGAALLKVMSAQVFSYLVP
                     WLLLLATLLFWQGRALLAWLGRDHHAVNRRWLAVGSWLVSVYGGFFGGGMGVMMMTLF
                     SLCGIPSLFTQNALKIFLSLVLNTASVIVLLAAGLAVWEHIPPLALGYVLGGYAGGRV
                     MNHLPQHWLRAGVVGFGLVMSGYFFWRAYL"
     gene            1324..3567
                     /locus_tag="PKN55_06215"
     CDS             1324..3567
                     /locus_tag="PKN55_06215"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNX70869.1"
                     /translation="MSRPSGSARLGAGADLIGESLACQRVRQLIERMAPTDRPLLIRG
                     PTGAGKEVVARRVHRLGIQPEAPFVDVNCAAIPENLVESELFGHARGAFTGASNYRAG
                     FFQQVGPGTLFLDEIGELPLALQPKLLRVLETREFRPVGSTDALPFHGRIVAATHRDL
                     AQMVRDGQFREDLYYRLAVFVVELPGLDQRKEDIPALVRHFAGQQSRALSFTPEAMQR
                     LYRQDWPGNVRQLRNLIDQLSVLAEDSRIDLATLAHFLPGQPEHTDLGDELADAVLKL
                     PGGDKLELVQDWLTDRALARCNGNKSAAAQLLGVSRKTVERRLKAREAQALELDAQLA
                     RGRQAVETGEFRSAIPLLRRASELAQDPLNRFEALRLLGISLRSVNGWLCPQAQACYQ
                     AAFEAGRDHADPQALSTLQFGLWASQLMTLSLGQARATAQDMLQRAQHLPMARMEAHV
                     AMANTLFWLGDGEETLACLARGDLLGGARPGGADMQGFDLSTLALTLEGLAAFQCGRF
                     AQARTAMDILHQRASAQHHTAFNRVMALQGAGWLAALFEATNQLAELGQALHTLAHSQ
                     ELDFYQGIGRILAGCARAAQGDSEGGETDIRLGYEQHIQRDGGVLFHSFQAWQRGDIL
                     LRAGQARQCLNLIGQAIDLALEHQERAYLGELLVVRARALWALGEPDVAEQELRGVIS
                     TAMALGSTPARLAAAHQLALLLRERGQFTLAQEALARALRGVEADGGAPVLARVQALQ
                     QALLAEY"
     gene            3848..4576
                     /locus_tag="PKN55_06220"
     CDS             3848..4576
                     /locus_tag="PKN55_06220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011136693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036361 - racemase activity, acting on
                     amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HNX70870.1"
                     /translation="MSMPPIRICVIAPAHSRTDTPPLLEALRPVTPPDVLVDIRHIRE
                     GSPCVENRTDWQENGMAVVRLARELAEEGYDGLWVADFDMCGVEAAREVVDIPVAGGF
                     VPAVFTALGLSGRFSILTLSQSTLAMQRSHARAYGVDGALASVRQLNCSPDDLNHTEL
                     VVNKALEQALRAVKEDGAEAIVLGHTGLIQVARRVSELLEDILGVYVPVIDPNQAGFG
                     FLLALTRMGLRPGRLTYTKVKLPS"
     gene            4751..6307
                     /locus_tag="PKN55_06225"
     CDS             4751..6307
                     /locus_tag="PKN55_06225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX70871.1"
                     /translation="MPIFRVYLRHPATTAPAWDYAETVVTACAAEAIAHAHAQWSHSR
                     PEVAVPALPHCQTQAIMIETSHLLAASTLSPGQQAAIHELERRVSHLLHDTLHGTFRL
                     VSAPAGLPMPLTEARGWRKGGLSTLDSLLTMAGNSQLTLSSERFSALYQHILRGIAFV
                     WSEQDRAILRREESQASDTLSAVLAASASLRPATIWPADDALQAVFDQLCRRFGAPEA
                     LPDSHAALIDALAGYRARAGESYALRRWRGLAQARLDAALNHTQQASADNGGAALAAG
                     GHQPAYLLDTPPATLWTQLCDTHQQVSLPLRLSDFTGEVVTVGLGSGMPFCLRVADVL
                     TLHAGEQHELDLSRYVRADSRVSLTLDWSGVCALRPRPLALDAQARQGWYAREILREV
                     LDLTWQPVSSLKLVNPDFDLNYLFGQGMAFSRLDHWVISQQPTLTLTLDRVDTARLRA
                     ELPTGAPARAQVLRLFNLGGGDSGWHVRELHIEGEHARLTLAAPAPDARTPRPAYVLG
                     GVPAFPPLGV"
     gene            complement(6580..8256)
                     /locus_tag="PKN55_06230"
     CDS             complement(6580..8256)
                     /locus_tag="PKN55_06230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002191185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008028 - monocarboxylic acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015718 - monocarboxylic acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate permease LctP family transporter"
                     /protein_id="HNX70872.1"
                     /translation="METWTQIYAPLGSLGLSALVASLPIIFFFIALAVLRMKGHVAGT
                     ITVAIALAIAVLVYQMPVDMAFAAAGYGFLYGLWPIAWIIVCSVFLYKVTVKTGQFDI
                     IRASVLSITEDQRLQMLLVGFSFGAFLEGAAGFGAPVAITAALLVGLGFNPLYAAGLC
                     LIANTAPVAFGAMGIPILVAGKVTSIDPFLIGKMAGHQLPLLSVIVPFWLVFMMDGFR
                     GIRQTWPAILVAGGSFAVTQFFTASYVGPELPDITSALVSLICLTVFLKVWHPKENFR
                     FANDGTTKGICVPRSGKAGSALGFTGGQVFKAWTPFIILTAMVTVWSLKPFKMLFDKG
                     GPLAGLVFKWEVPHLHNLVIKAAPIVASAKPYEAIYKLDILSATGTAILISALISIAL
                     LRMKPADAARTFAEVVLELKRPIYSIGMVLAFAFVANYSGLSSTLALVLAGTGALFPF
                     FSPFLGWLGVFLTGSDTSSNALFCSLQATTAHQIGVSDTLLVAANTTGGVTGKMISPQ
                     SIAVACAAVGLVGKESDLFRFTVKHSLMFATMVGIITYVQAYYLTWMIPQ"
     gene            complement(8654..>9795)
                     /locus_tag="PKN55_06235"
     CDS             complement(8654..>9795)
                     /locus_tag="PKN55_06235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011136574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HNX70873.1"
                     /translation="GLSLTPRQRIVLWRRINHLQRSGEAPAELSSHQSQYRYLGIDTC
                     AATGMCATRCPVGINTGTLMKQLKGASPRPDAARWAAGHMRLLTAGARLGLGVRASAQ
                     KLLGKPAVQALNRSLHHTFKTIPIVPLGAPGPARRLPTPSAQGEPVVYFVSCVNRTLA
                     EGRDGAASVAEHTLSLFAKAGFAARYPDNLDALCCGQPFDSKNAADAAELASSQTAKA
                     LLAASDNGRIPVYIDNGPCAERLITAQREGRVDARLKLYDAASFLSEFIAPRLNFTRQ
                     LDQLALHVPCSASKLGVGGKLTALARRCATQLSVPDIACCGFAGDKGFTTPELNANSL
                     KRLQLPAGCAHGVSMSRTCQIGLTTHTGVDYQSIEALLDACSDPA"
BASE COUNT         1671 a         3181 c         3265 g         1678 t
ORIGIN      
        1 gtggaccgcg ccgaccatgc cgaccacgcc atccgcgaaa tcggcgaggc ttcggccaac
       61 acggtgacca tggtcaacga aatctcggcg gccatccgcg aacaaagcca ggccagctcc
      121 agcatcgccg aacaggtcga gcgcatcgcc cgcatggcgc aaaccgccag tggcgccgcc
      181 ggcaacgccg cgcaagccgc cgattcgctg gaaaaactgg ccagccagca acgcgatacc
      241 ctgggccact acacggtata attctgcgcc agccacggcg gaatcggtcc gcctggcctg
      301 actgcggagc ccgccatgct gcttgccctg gccctgtttg ccgccgccct gctggccggc
      361 gtggtcaacg ccttagccgg cggcggcctg ttgttcacct ttccggcgct ggtggcgctg
      421 ggcgtgccgc cagtggcggc caacgccacc gccaatatcg cctcctggcc gggctacgcc
      481 tcggcggcat tcgccatgcg ccgcgagctg gcccacgctg gcgggctgcg cctgctgctg
      541 atctccggcg tggccggcgg cgtcactggc gcggcgttgc tcaaggtgat gtcggcgcaa
      601 gtgttcagtt atctggtgcc ctggctgttg ttgctggcca cgctgttgtt ctggcagggc
      661 cgcgcgctgc tggcctggct gggccgcgac caccacgccg tcaaccgccg ctggctggcg
      721 gtgggcagct ggctggtcag cgtgtatggc gggttttttg gcggcggcat gggcgtgatg
      781 atgatgacgc tgttcagcct gtgcggcatt ccttcgttat tcacccagaa cgccctgaaa
      841 atctttctgt cgctggtgct caacaccgcc tcggtgatcg tgctgctggc cgctggcctg
      901 gcggtgtggg agcacatccc gccgctggcg ctgggttatg tactgggcgg ctacgccggc
      961 gggcgggtga tgaaccacct gccgcaacac tggctgcgcg ccggcgtggt gggttttggc
     1021 ctggtgatga gcgggtattt cttctggcgg gcgtatctgt gagttgcgct tgcccggcct
     1081 gcgccgccag accacgccag cccaagtcca aaaccggctg acgcccggca gtgccgggct
     1141 gttatcacct gtcattccag atcaggcacg gctgacgtat gattctcggc cgccggggcg
     1201 cgccgcgccc cggcctgctg tcgcccacct ggcggtggcg atgtgcttgc ttcaggagac
     1261 cctggccatg ctgttgtcga tgcgccccct tgccttttcc gtgttgttgc catgtctgcc
     1321 cgcatgagcc gcccatccgg cagcgcccgc ctgggcgcag gggcggacct gattggtgaa
     1381 tcactggcct gccagcgtgt gcgtcagctg atcgagcgca tggccccgac tgaccggccc
     1441 ttgctgattc gtggcccgac cggcgccggc aaagaggtgg tggcgcgccg agtacaccgg
     1501 ctgggcatcc agccagaagc tccgtttgtg gacgtcaact gcgcggcgat tccggaaaac
     1561 ctggtggaat ccgagctgtt tggccatgcc cgtggggcat ttactggtgc gtccaattat
     1621 cgcgccggtt ttttccagca ggtggggccg ggcacgctgt ttctggatga aatcggcgaa
     1681 ctgccgctgg cgctgcaacc caagctgctg cgcgtgctgg aaacccgtga gttccgcccg
     1741 gtaggcagca cggatgcgct gccgtttcat ggtcgcatcg tggccgccac ccaccgggac
     1801 ttggcgcaga tggtgcgcga cggccagttt cgtgaagatc tgtattaccg gctggcggtg
     1861 tttgtggtgg agctgcctgg cctggaccag cgcaaggaag acatccccgc cctggtgcgt
     1921 cactttgccg gccagcaaag ccgggcgctg agttttaccc ccgaagccat gcaacggctg
     1981 tatcgccagg actggccagg caatgtgcgc cagctgcgca atctgatcga ccagctcagc
     2041 gtgctggccg aagacagccg catcgacctg gccacgctgg cgcattttct gcccggccaa
     2101 cccgaacaca ccgacctggg cgacgagctg gctgacgccg tgctgaaatt gccaggcggc
     2161 gacaaactgg agctggtgca ggactggctg accgaccgcg ccctggcgcg ctgtaatggc
     2221 aacaaatccg ccgcagccca gctgctaggc gtcagccgca agacggtgga acgccggctg
     2281 aaggcacgcg aagcacaggc gctggagctg gacgcccaac tggcacgtgg ccgtcaggcc
     2341 gtggaaaccg gcgaatttcg cagcgccatt ccgctgctgc gccgtgccag cgagctggca
     2401 caagatcctc ttaaccgctt tgaagcactg cgtctgctgg gcatcagcct gcgcagcgtc
     2461 aatggctggc tgtgtccgca agcacaagcc tgctatcagg ccgcgtttga ggccggacgc
     2521 gatcatgctg acccgcaagc cttgtccact ttgcaatttg gcctgtgggc cagccaactg
     2581 atgacactga gcctgggcca ggcacgcgcc accgcccagg acatgctgca acgcgcccag
     2641 cacctgccga tggcacgcat ggaagcccat gtcgccatgg ccaacaccct gttctggctg
     2701 ggggacggcg aagaaacctt ggcttgcctc gcccgcggag atctcctggg aggggcacgc
     2761 cccggcggcg ccgatatgca gggctttgac ctcagcaccc tggcgctgac gctggaagga
     2821 ttggccgcgt ttcaatgcgg acgctttgcc caggcgcgca ccgccatgga tattttgcac
     2881 cagcgcgcca gcgcccagca tcacaccgcg ttcaaccggg tgatggcctt gcagggtgcg
     2941 ggctggctgg ccgccctgtt tgaagccaca aaccagttgg ccgaactggg ccaggcgcta
     3001 cacacactgg cacatagcca ggagctggat ttttatcagg gcatcggccg catcctggcc
     3061 ggctgtgccc gcgccgccca aggcgacagc gaaggcggcg agactgatat ccgtctgggc
     3121 tatgaacagc atatccagcg tgacggtggc gtgctgttcc actcgttcca ggcctggcaa
     3181 cgcggcgata tcctgctgcg cgccgggcag gcgcgtcagt gcctgaacct gattggccag
     3241 gcgattgatc tggcgctgga gcaccaggag cgagcctatc tgggcgagct tctggtggtg
     3301 cgtgcccgtg cgctgtgggc gctgggcgag ccggatgtgg ccgaacaaga actgcgcggg
     3361 gtgatttcca ccgccatggc gctgggctcc accccggcgc ggctggccgc cgcccaccaa
     3421 ctggcgctgc tgctgcgtga gcgtggtcag ttcacgctgg cacaagaggc cctggcgcgg
     3481 gcgctgcgcg gcgtagaggc cgacggcgga gcgcccgtgc tggcgcgggt gcaggccctg
     3541 caacaggctt tgctggccga atactgattc ccagatcaag caaaggcaga aaccatatct
     3601 cggccatggc catcctggta tcgcggcgga cgaaatcgtc cagcatgccg gcggataaat
     3661 aggcgctttc gtccaccggc atgacttgcc ggacaggcca atctgttgca aaacaagcct
     3721 gtatgtaaat ggcacgcgag ttgcaatagc caaaaccgtt accggccagc tggcccggcg
     3781 taccttgtct gcgcccggga caacacccgt ccggactctt gctgcctttt taccggagcc
     3841 ccgattcatg agcatgcccc cgatccgcat ttgcgtcatc gcccccgccc attcacgcac
     3901 cgatacccct ccgctgctgg aggcgctgcg gcccgtgacg ccgcctgatg tgctggtcga
     3961 tatccggcat atccgcgaag gcagcccgtg cgtggaaaac cgcaccgact ggcaagaaaa
     4021 cggcatggcc gtggtgcggc tggcgcgcga gctggctgag gaaggctacg atggcctgtg
     4081 ggtggcagac tttgacatgt gtggcgtcga ggccgcccgc gaggtggtcg atatacccgt
     4141 ggctggcggt tttgtgccgg cggtgttcac cgccttgggc ctgagcgggc ggttttccat
     4201 cctcaccctg tcgcaaagca cactggccat gcagcgcagc catgcccgcg cctatggcgt
     4261 ggatggcgcg ctggcctcgg tgcgccagct caattgcagc ccggacgacc tgaaccacac
     4321 cgaactggtg gtcaacaagg cgctggaaca ggctttgcgc gcggtcaaag aggatggcgc
     4381 cgaagccatc gtgctgggcc acaccgggct gattcaggta gcgcggcgcg tcagcgaact
     4441 gctggaagac atcctgggcg tgtatgtgcc ggtcatcgac cccaatcagg ccggttttgg
     4501 ctttttactg gcgctgaccc gcatgggcct gcgccccggc cggctgactt acaccaaggt
     4561 aaaattgccc agctgaatca ggctggcatg ctttgcgcaa tatacactga cgctggcgtc
     4621 aaaactggca aaaaatggac gttttcgtcc attccccctg taaaagccgg gcgttttcgt
     4681 ccaagcccgg ctgataacgt ccactttgca ccgagttttg ccgcggatgc ctgtttgaag
     4741 gacctggccc atgccgattt ttcgcgtcta cctccgccac ccagccacca ccgcccccgc
     4801 ctgggattat gccgaaaccg tggtcaccgc ctgtgccgcc gaagccattg cccatgccca
     4861 cgcccagtgg tcacacagcc gcccggaagt ggccgtgccg gcgctgccgc actgccagac
     4921 acaagccatc atgatcgaaa ccagccatct gctggcggcc agcacgctgt ccccaggcca
     4981 gcaagccgcc attcatgagc tggagcgccg ggtcagccat ctgctgcacg acaccctgca
     5041 tggcactttc cgtctggtca gcgcgcccgc cggcctgccc atgccgctga ccgaggcgag
     5101 gggctggcgc aagggcgggc tcagcacgct ggacagcctg ctgacgatgg ccggcaatag
     5161 ccagctgacc ttgtccagcg agcggttttc tgcgctgtac cagcatattc tgcgcggcat
     5221 cgcctttgtg tggagcgaac aagaccgcgc cattctgcgc cgcgaagaaa gccaggccag
     5281 cgatacgcta tctgccgtgc tggccgccag cgccagcctg cgcccggcca ccatctggcc
     5341 agcagacgat gccttgcaag ccgtgttcga ccagctctgc cgccggtttg gcgcgccaga
     5401 agctctgccg gacagccacg cggccttgat cgacgcactg gccggctatc gcgcccgggc
     5461 gggcgaaagc tatgcgctgc gccgctggcg tggtctggcg caggcgcgac tggacgcggc
     5521 gctgaaccac acccagcagg ccagcgccga caacggcggc gccgccctgg cggctggcgg
     5581 ccaccagccg gcgtatctgc tggatacccc accggccacc ctgtggaccc agctgtgcga
     5641 cacccaccag caagtcagcc tgccgctgcg gctgagtgat tttactggcg aggtggtcac
     5701 cgtgggtctg ggcagcggca tgcctttctg tctgcgtgtg gccgatgtgc tcaccctgca
     5761 cgcgggcgaa cagcacgaac tggacctctc gcgctatgtg cgagccgaca gccgggtcag
     5821 cctgacactg gactggtctg gcgtatgcgc cctgcgcccg cgcccgctgg cgctggatgc
     5881 ccaggcgcgt cagggctggt atgcgcgtga aattctgcgt gaagtgctgg acctcacctg
     5941 gcagccagtc agcagcctga agctggtcaa cccggatttt gacctcaatt acctgtttgg
     6001 ccagggcatg gcgttttccc ggctggatca ctgggtgatc agccagcaac ccacactgac
     6061 gctgacgttg gatcgggtgg acaccgcacg cctgcgcgcc gagttgccaa caggcgcgcc
     6121 agcccgtgcc caggtgttgc gcttgttcaa tctgggcggc ggcgacagtg gctggcatgt
     6181 gcgcgagctg catatcgagg gtgaacacgc ccgcctgacc ctggccgccc cggccccgga
     6241 tgccagaaca ccccggccgg cctatgtgct gggcggcgtg ccggccttcc cgccgctggg
     6301 cgtgtaagca taaaaaaacc gcccaggccg gggagccatg ggcggacaag acacgttccg
     6361 gcattgcgcc ggcgcggaaa cacactggtg cagcctgtct ggatcgtcag gccgaaaaaa
     6421 tctagcgctg cgcgcggaat gttgagcgag gctggcagca atgcttagcg tcgctcacca
     6481 cacccagcgc agcgcgcctg gctaggcgtg cggccgcaga cagtacaagc agtacggcaa
     6541 ggccgcgcaa cgacgccagg cgggtttggt gagcggccct tactgcggaa tcatccacgt
     6601 caggtaatac gcctgcacat aggtgatgat gcccaccatg gtggcaaaca tcaggctgtg
     6661 cttgacggtg aagcggaaca gatcagactc cttgcccacc aggcccacag ccgcgcaagc
     6721 cacggcgatg gattgcggcg agatcatctt gccggtcacg ccgcccgtgg tgttggccgc
     6781 caccagcaag gtgtcggaca cgccaatctg gtgcgcggtg gtggcttgca gcgagcagaa
     6841 cagcgcgttg gacgaggtgt ccgaaccggt caggaacacg cccagccagc ccaggaacgg
     6901 cgagaagaac gggaacagcg cgccggtgcc agccagcacc agggccaggg tggacgacag
     6961 gccggagtag ttggcgacaa aggcaaacgc cagcaccatg ccaatcgagt agatcgggcg
     7021 cttcagctcc agcaccactt cggcaaaggt gcgggcggcg tcagccggct tcatgcgcag
     7081 cagggcgatg gaaatcagcg ccgagatcaa aatggcggtg ccggtggccg acaggatgtc
     7141 cagcttgtag atcgcctcgt agggcttggc gctggccaca atcggggcgg ccttgatcac
     7201 cagattatgc aggtgcggca cttcccactt gaacaccagg ccagccagcg ggccgccctt
     7261 gtcgaacagc atcttgaacg gcttcaggct ccacaccgtg accatggcgg tcaggatgat
     7321 gaacggggtc caggccttga acacctggcc gccggtaaag cccagcgccg agccggcctt
     7381 gccgctgcgc ggcacgcaaa tgcccttggt ggtgccgtcg ttggcaaagc ggaagttttc
     7441 tttcgggtgc cacactttca ggaacacggt caggcagatc aggctgacca gggccgaggt
     7501 gatgtccggc agttccgggc cgacatagct ggcggtaaag aactgggtca cggcaaacga
     7561 accgccggcc accagaattg ccggccaggt ctggcggatg ccacggaaac cgtccatcat
     7621 gaacaccagc cagaacggca cgatcaccga cagcagcggc agttgatggc cagccatttt
     7681 gccgatcagg aacggatcaa tgctggtcac cttgccggcc accagaatgg ggatgcccat
     7741 ggcgccaaac gccaccgggg cggtgttggc gatcaggcac aggccagcgg cgtacagcgg
     7801 gttgaagccc aggccgacca gcagcgcggc ggtgatggcc accggggcgc caaaaccggc
     7861 cgcgccttcc aggaaggcgc cgaacgagaa gcccaccagc agcatttgca ggcgttggtc
     7921 ttcggtaatc gacaacaccg aggcgcggat gatgtcaaat tgcccggttt tgaccgttac
     7981 tttgtatagg aacaccgagc agacaataat ccaggcaatc ggccacagac cgtacaaaaa
     8041 gccataacca gcggcggcaa acgccatgtc caccggcatt tgatacacca gcacggcaat
     8101 cgccagggcg atggccacgg tgatggtgcc ggccacatgg cctttcatgc gcagcaccgc
     8161 cagcgcgata aagaagaaaa tgatcggcag cgaggccacc agcgccgaca ggcccaggct
     8221 gcccagcggc gcgtagattt gcgtccaggt ttccatgaaa caatagtcct ccgcgcagcc
     8281 ccgaaatggg gttgcgacac gatgaagtga agtgtccggc cgggtgggcc gaaggagtgc
     8341 gcaagggccg gctttgccgt cggtgacggc ggggccaagc ctgcatcctc gtgcgttgcc
     8401 ggggtcagcg ggcaacgtcc gggcctgggc gacagcggct cagggtggct gaaccgggcc
     8461 gtcaggcgcc tggcgaggcg gcgctagcgc agcggggcgg tcggtgtggg catgttttct
     8521 cctcagcggg tttgtgggcc ggctggcttg cgccgtcggt ctctttacgc ggcgcatctt
     8581 ttccctgacg ggtttgggtg cgccgctttt aattcacgcg ccgcctgggc ggcgcgcaag
     8641 gtgttgcaat atctcaggcc gggtcgctac aggcgtccag cagagcttcg atgctttggt
     8701 aatccacgcc ggtgtgcgtg gtcaggccaa tctgacaggt gcggctcatc gacaccccgt
     8761 gcgcacagcc ggccggcagt tgcaggcgct tgaggctgtt ggcgttcagc tcgggggtgg
     8821 tgaagccctt gtcgccggca aagccgcaac aggcgatgtc cggcacgctc agctgcgtgg
     8881 cgcagcggcg cgccagcgcg gtgagcttgc cgcccacgcc cagcttgctg gcgctgcacg
     8941 gcacatgcag ggccagttgg tcgagctggc gggtgaagtt caaacgcggg gcgatgaact
     9001 cgctcaaaaa gctggcggcg tcgtacagct tgagccgggc atcgacgcgg ccttcgcgct
     9061 gggcggtaat cagccgctcg gcgcaggggc cgttgtcgat gtacaccggg atgcggccgt
     9121 tgtcgctggc ggccagcagc gccttggcgg tctggctgct ggccagctcg gcggcgtcgg
     9181 cggcgttttt tgagtcaaac ggctggccgc agcacagcgc gtccagattg tccgggtagc
     9241 gggcggcaaa gccggccttg gcaaacaggc tcagcgtgtg ttcggccacg ctggcggcgc
     9301 cgtcgcggcc ttcggccagg gtgcgattca cgcagctgac gaagtacacc accggctcgc
     9361 cctgcgccga cggcgtgggc aggcggcggg ccgggccggg cgcgcccagc ggcacgatgg
     9421 gaatggtttt aaaggtgtga tgcaggctgc ggttcagcgc ttgcaccgcc ggcttgccca
     9481 gcagtttttg cgcgctggcg cgcacgccca ggcccagccg cgcgccagcg gtgagcagac
     9541 gcatatggcc ggccgcccag cgcgccgcat ccgggcgcgg gctggcgccc ttgagctgct
     9601 tcatcagcgt gccggtgttg atgcccaccg ggcagcgggt ggcgcacatg ccggtggcgg
     9661 cgcaggtgtc gatgcccaga tagcgatact ggctttggtg gctgctcagc tcggccggcg
     9721 cttcgccgct gcgctgcaaa tggttgatgc gccgccacag cacaatgcgc tggcgcgggg
     9781 tcaggctcag gccgt
//
LOCUS       DAOPYX010000091         9786 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_8147_length_9786_cov_10.091563, whole genome shotgun sequence.
ACCESSION   DAOPYX010000091 DAOPYX010000000
VERSION     DAOPYX010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 9786)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9786)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9786
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8147_length_9786_cov_10.091563"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(90..1166)
                     /gene="mnmA"
                     /locus_tag="PKN55_06240"
     CDS             complement(90..1166)
                     /gene="mnmA"
                     /locus_tag="PKN55_06240"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012697291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HNX70874.1"
                     /translation="MTRPQRIVVGLSGGVDSSVTAWLLKQQGHEVIGVFMQNWEDDNN
                     DEYCSIKQDALDAMAVADIIGIDMEVVNFAGEYKDRVFRYFLEEYAAGRTPNPDVLCN
                     AEIKFKAFLDYAVSLGAEAIATGHYACRVDSPRGAELHKGADPNKDQSYFLYRLQPHQ
                     LRPALFPLGELHKPQVREIASQIKLPNARKKDSTGICFIGERPFREFLQRYLPTQPGE
                     MVTPEGKVVGQHQGLMYYTLGQRQGLGIGGPGEPWFVAGKQRDANQLLVVQGHDHPLL
                     YSPALCADQLSWTLGEPPAPGRYAAKNRYRMQDAACTLDYDANGRAVLTFDQPQWAIT
                     PGQSAVLYDGSLCLGGGVIVGAPE"
     gene            complement(1163..1717)
                     /locus_tag="PKN55_06245"
     CDS             complement(1163..1717)
                     /locus_tag="PKN55_06245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014086515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX70875.1"
                     /translation="MPALRHLGLCLALLLPLAAEWPAHSETLPICYNYGCKQRGEAVL
                     GPLDNDQLASLLDQPDAEGERAAVGQAIGLMNQIAARTTPIAADRGGNVQDSGDGSQD
                     CIDHSRNATTYLHYLLEHGWLRFHRVLEPAWRAPWLVDLHYAAVIEDLKDGLRYAVDA
                     WFFDHGHPAAVLPLPDWNKGFSPQ"
     gene            complement(1749..2213)
                     /locus_tag="PKN55_06250"
     CDS             complement(1749..2213)
                     /locus_tag="PKN55_06250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011136948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HNX70876.1"
                     /translation="MVRELQWKPNATVAAVVERDGEFLLVEEHTEAGVRLNQPAGHWE
                     HGESILDAVRRETLEETGYHFEPTALLGVYVSPRPDSEVVYLRFAFVGQITGHDSQRA
                     LDAGIIAARWLSRDAALADPARLRSPMVSRCIEDYLSGQRYPLDLIRAVPAG"
     gene            2501..2701
                     /locus_tag="PKN55_06255"
     CDS             2501..2701
                     /locus_tag="PKN55_06255"
                     /inference="COORDINATES: protein motif:HMM:NF016230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HNX70877.1"
                     /translation="MYVCVCHAVTDSQVRQAVHNGVTLMRELREELNVASSCGRCACC
                     ARDVLRATLKEIGEREDFAQAA"
     gene            complement(2771..3217)
                     /gene="dut"
                     /locus_tag="PKN55_06260"
     CDS             complement(2771..3217)
                     /gene="dut"
                     /locus_tag="PKN55_06260"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019922890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HNX70878.1"
                     /translation="MNVDVKILDPRLHDHPPAYATPGSAGLDLRAAIDADLLIQPGET
                     HLVPTGLAIHLADPGYAAIILPRSGLGHKHGIVLGNLVGLIDSDYQGQLFVSVWNRSD
                     APFNLAPLERMAQLVIVPVVQAQFNVVEEFAASERGEGGFGSTGKR"
     gene            complement(3338..4522)
                     /gene="coaBC"
                     /locus_tag="PKN55_06265"
     CDS             complement(3338..4522)
                     /gene="coaBC"
                     /locus_tag="PKN55_06265"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HNX70879.1"
                     /translation="MTAQHILLGITGGVAAYKSAELVRLLVKAGHTVDVAMSESATRF
                     VTPVTFQALSGRPVYVDLWDARPDNNMAHIELTRRADCFLIAPATADVLARLAHGHCN
                     DLITTLAAARTCPLAVAPAMNKQMWDNPPNQRNIAQLRADGVTVFGPGHGEQACGEVG
                     DGRMLEPEQLFDLLTGLTQAKTLAGRRVLLTAGPTFEPIDPVRGITNLSSGKMGYALA
                     RACRDAGAEVTLISGPTALPRPAEVHFIAAGSALSMHAAVMEAVPHHDVFIAVAAVAD
                     YRVANASAEKLKKTPGQAPTLTLTENPDILASVAALPSAPFCVGFAAESHNLLAHAQA
                     KRERKRVPLLVANLAQHAMGADDNEIVLLDDHGQHPLPRMDKTGAARAIVAHLAGMLP
                     IR"
     assembly_gap    4541..4640
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4996..6870
                     /locus_tag="PKN55_06270"
     CDS             4996..6870
                     /locus_tag="PKN55_06270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008953822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNX70880.1"
                     /translation="MINSLRHRLVFFVLLVVTVLAAVLTTVAYQHTYAAAEAGVRQNI
                     AATTANKVAFINEWVHSRQRVVGSVLPRFGHGELKPVLDQALEAGGFDDMYVGQPDKT
                     MTQFSKATLVPAGYDPTVRPWYVAAAATEGPIASPPYIDASTKQPIITFAQGLRRNGQ
                     LVAVAGGDVTLKRVVEEVTAAQLPGNGYAFLITQDGAVIAHPAADSALKKISEVVPGL
                     TPDSVPRDGAIHTTTLNGVKYFMVLQPVGKTGWLMGALVPHDAALAPVQHMLYTLLGV
                     LAACIALASVAAWLGIAQLMRGLASMRDAMRSVASGSGDLTLRLPVKGNDELAEIAQA
                     FNQFVAALQQMFTQVRQYAESLADTAEQLDRTAGQIAEDSRSQASELANTAATIEQVT
                     VSINHIAEHVGETEALVTRSRQGSISSQDGMEAVAQEMHAVVGAIASLRSVMDGLSSK
                     SEEIRGIVNTIHDIADQTNLLALNAAIEAARAGEQGRGFAVVADEVRKLAERTSTATV
                     QIASIMDQVIDQTGDAAEHVGSTQQRVTQSMRHTEEAAGRVARVREHTDDIAQRMANI
                     KMATAEQGSATHDMARSAERVNAKTQQTDGNLQQVLGTLHGLAQCGESLKALVSRFKL
                     "
     gene            complement(6946..7722)
                     /locus_tag="PKN55_06275"
     CDS             complement(6946..7722)
                     /locus_tag="PKN55_06275"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012697168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNX70881.1"
                     /translation="MRTKLLCGLIAAVLTLPAMADKPAFSVNGQAVSQARVDAMVQQL
                     QQQGQQDSPQLRAMVRDQLVLTELLRQEAQKKGLDKSAEYKSEMDAYAARLLANFYVR
                     DWMRANPVSDAQLRAEYDKLVAQLARKEYSARHILVENEADARKILEDLKKGKAFTAI
                     AKEVSKDTGSKDKGGELGWAPAEAYVKEFSEALTKLTPGQVTANPVKTQFGWHIIQLV
                     DARTQQGPSFDELKPQLAQRIQGAQLDAYLSKLKQQAKVE"
     gene            complement(7744..8538)
                     /locus_tag="PKN55_06280"
     CDS             complement(7744..8538)
                     /locus_tag="PKN55_06280"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012697169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNX70882.1"
                     /translation="MRLNHIAAILLTLSLAAGAQAADKTVAVVNGASIPQSELDQLAD
                     SVVKGSNGRVKDSPELRAELKQQLINRQIILQEASRRSLDKSAQFKDRLEDIRNDLLQ
                     QALFEDIARANPVTDAQVKAEYDRLKAQTVGQKEVHARQIVLGSEEEAKQVIAQLKKG
                     GKFEDVARAKSKDPAAKQTGGDMGWGNLGMMPKPLADELKSIAKGQISAQPFKTELGF
                     HVFKIEEARDVKVPSFDEAKQQLARQMQNAQIEKTLVELRNKAKIQ"
     gene            complement(8576..8851)
                     /locus_tag="PKN55_06285"
     CDS             complement(8576..8851)
                     /locus_tag="PKN55_06285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012697170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family protein"
                     /protein_id="HNX70883.1"
                     /translation="MSEALSLDALIHQRLAALTPQEIHLHDDSAAHAGHAGAASGGGH
                     FELTLVSPVFAGVNAVARHRLVYQALGDLIPSRIHALSLRAYAPGEL"
     gene            complement(8853..9149)
                     /locus_tag="PKN55_06290"
     CDS             complement(8853..9149)
                     /locus_tag="PKN55_06290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006717394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HNX70884.1"
                     /translation="MRYVIIGHDVPNSLEQRLAARPEHLARLTALRDAGRLTLAGPCP
                     AIDSADPGPAGFVGSVIVADFDSLQDARAWADADPYVAAGVYARVDILPFKQVF"
     gene            complement(9149..9682)
                     /locus_tag="PKN55_06295"
     CDS             complement(9149..9682)
                     /locus_tag="PKN55_06295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012697172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HNX70885.1"
                     /translation="MKFLFDLFPVILFFAAFSLTRDIYLATAVAMAATVGQVAFSWFK
                     HRKVDTMQWVSLGLIVVLGGATLLLHDKSFIMWKPTVLYWLMGVGLLVSQAMGKQPLK
                     SLMGKQLTLPEAVWRRLSLAWVAFFAGMGALNLYVAFTFSEEVWVNFKLFGGMGLMLL
                     FVLGQSVVLARYMEDSD"
BASE COUNT         1720 a         2997 c         3154 g         1815 t
ORIGIN      
        1 atcaagttag gggattgccc gtcacgcgcc agggaggccg tgacgggttt ttttgttgaa
       61 gccaacgccc gcaggtaggg taaatccgct tactccggcg cgccgacaat caccccgccg
      121 cccaggcaca ggctgccgtc gtacagcacg gcggattggc cgggggtgat ggcccattgt
      181 ggctggtcga aggtcagcac ggcgcggccg ttggcgtcgt agtccagcgt gcaggcggcg
      241 tcttgcatgc ggtagcggtt tttggcggcg tagcggccgg gcgctggcgg ctcgcccagc
      301 gtccaggaca attggtcggc gcacagcgcc gggctgtaca gcaacgggtg gtcgtggcct
      361 tgcaccacca gcagctggtt ggcgtcgcgc tgtttgccgg ccacaaacca cggctcgccc
      421 ggcccgccga tgcccaggcc ctgacgctgg ccgagggtgt aatacatcag gccctggtgc
      481 tgacccacca ccttgccttc cggggtgacc atctcaccgg gctgggtggg caggtagcgc
      541 tgcaagaatt cgcggaacgg ccgttcgccg ataaagcaga tgccggtgct gtcttttttg
      601 cgggcgttgg gcagcttgat ctggctggcg atctcgcgca cctgcggctt gtgcagttcg
      661 cccagcggaa acagcgccgg gcgcagctgg tgcggttgca gccggtacaa aaaatagctt
      721 tggtctttgt tggggtctgc gcctttgtgc agctcggcgc cgcgcgggct gtccacccgg
      781 caggcgtagt ggccggtggc gatggcttcg gcgcccaggc tgacggcgta atccagaaac
      841 gccttgaact tgatttcggc attgcacagc acgtccgggt tgggggtgcg gccggcggcg
      901 tattcttcca gaaaatagcg aaacacccgg tctttgtatt cgccggcaaa gttcaccact
      961 tccatgtcga tgccgatgat gtcggccacc gccatggcgt ccagcgcgtc ctgcttgatc
     1021 gagcagtatt cgtcgttgtt gtcgtcttcc cagttttgca tgaacacccc gatcacttca
     1081 tggccttgct gcttgagcag ccaggcggtg accgaagaat ccacgccgcc ggataaaccg
     1141 acgacaatgc gttgcgggcg ggtcattgcg gagaaaatcc tttgttccag tccggcagcg
     1201 gcagcaccgc cgccgggtgg ccgtgatcaa aaaaccaggc gtctacggca tagcgcaggc
     1261 cgtctttcag gtcttcaatc acggcggcgt aatgtaagtc caccagccac ggcgcgcgcc
     1321 aggcgggctc cagcacccga tgaaagcgca gccagccgtg ctccagcaga tagtgcagat
     1381 aggtggtggc gttgcggctg tggtcaatgc aatcctggct gccgtcgccg ctgtcctgaa
     1441 cattgccgcc ccggtcggcg gcaatcggcg tggtgcgcgc ggcgatctgg ttcatcagcc
     1501 cgatggcctg gcccacggcg gcgcgttcgc cttcggcgtc gggttggtcc agcaggctgg
     1561 ccagttggtc gttgtccagc gggcccagca ccgcttcgcc acgctgcttg cagccgtagt
     1621 tgtagcagat gggcagggtt tcgctgtgcg ccggccattc ggcggccagc ggcagcagca
     1681 gggcaagaca caggccaaga tggcgcaagg caggcatgac attctccggc gctcagcgcc
     1741 gggcagggtc agcccgccgg cacggcgcga atcaagtcca gcgggtaacg ctggccagac
     1801 agataatctt caatacagcg agacaccatg gggctgcgca gccgggccgg atcggccagc
     1861 gccgcatcgc ggctgagcca ccgggcagcg ataataccgg catccagcgc gcgctggcta
     1921 tcgtggccgg tgatctggcc gacaaaggca aagcgcaggt aaaccacttc gctgtccggg
     1981 cgcgggctga catacacgcc cagcagcgcg gtgggttcaa agtgatagcc ggtttcctcc
     2041 agggtttccc gccgcacggc gtccaggatg gattcgccgt gttcccaatg gccggctggc
     2101 tgattcagcc gcacgccggc ttcggtgtgt tcttccacca gcaaaaactc gccgtcgcgc
     2161 tcgacaacgg cggcgacggt ggcgttgggc ttccattgca attctcggac catctgtctt
     2221 gtatccttct ggggggcggc gcgtggcaaa tccgacacaa cgccgtcagt cgccaggcat
     2281 gggcgggctt gcctggcaat tcaatcaatc aaatcagtat cttgacaaaa atatgggttc
     2341 agcagcttct ggcgtgattt atgctggatg gcgagtgaag gctgcgtagg tttgtctacg
     2401 cacagaaata ttgacaacta ttctcgttta ttggatagtt gcgatacgtt ctcattcact
     2461 aagtgggcca gcgcgcccgc cccgcttcag gagccatgcc gtgtacgtct gcgtttgcca
     2521 tgccgtgacc gatagccagg ttcgtcaagc cgtgcacaac ggcgtcaccc tgatgcgcga
     2581 gctgcgcgaa gagctgaatg tcgccagcag ctgcggccgt tgcgcctgct gcgcccgcga
     2641 tgtgctgcgc gccacactga aagaaatcgg cgagcgcgaa gacttcgccc aggctgcctg
     2701 atcagttttt ggcggttttt ttgggcaagg cgctcaggcg gcgccgggcc gcctgagctg
     2761 gcgccggcgc ttaacgcttg ccggtgctgc caaatccccc ctcgccgcgc tcactggcgg
     2821 caaactcttc caccacattg aactgtgcct gcaccaccgg cacgatcacc agctgcgcca
     2881 tgcgctccag cggcgccaga ttaaacggcg cgtcgctgcg gttccacacc gacacaaaca
     2941 gctgcccctg gtagtccgaa tcaatcagcc ccaccagatt gcccagcaca atgccgtgct
     3001 tatgccccag ccccgagcgc ggcaggatga tggccgcgta gccggggtcg gccaggtgaa
     3061 tggccaggcc ggtgggcacc agatgggttt cgcctggctg aatcagcaaa tcggcgtcga
     3121 tggcggcgcg caggtccaga ccggccgagc cgggggtggc gtaggccggc gggtggtcgt
     3181 gcaggcgggg gtcgagaatt ttaacgtcaa cgttcatgac aaagtccggg tcaagcagaa
     3241 tgggggatgc gttgcgccag gcgcaacgcg agggcgccag cggggcaagg tggcccgcgc
     3301 caggcaagac agcgcgcccg catcgggcgc gcggggttca gcggatcggc agcatgccgg
     3361 ccaggtgggc cacaatggcc cgcgccgcgc cggttttgtc catgcgcggc agcgggtgct
     3421 ggccgtggtc gtccagcagc acgatttcat tatcatcggc gcccatggcg tgttgcgcca
     3481 ggttggccac cagcagcggc acgcgcttgc gctcgcgctt ggcctgggca tgcgccagca
     3541 gattatggct ttcggcggca aagcccacgc agaacggcgc gctgggcagg gccgccacgc
     3601 tggccaggat gtcggggttt tccgtcaggg tcagcgttgg cgcctggccg ggggtttttt
     3661 tgagtttttc ggcgctggcg ttggccaccc gataatcggc caccgccgcc acggcgataa
     3721 acacatcatg gtgcggcacg gcttccatca ccgccgcgtg catggagagc gcgctgccgg
     3781 cagcaataaa gtgcacctca gccgggcgcg gcagcgcggt ggggccagaa atcagcgtga
     3841 cctcggcgcc cgcgtcgcgg caggcgcgcg ccagcgcgta gcccatcttg ccgctggaca
     3901 ggttggtgat gccgcgcacc gggtcgatgg gctcaaaggt ggggccggcg gtcagcagta
     3961 cgcggcggcc ggccagcgtt ttggcttggg tcagtccggt cagcaggtcg aataactgtt
     4021 cgggttccag catgcggccg tcgcccacct cgccgcaggc ttgttcgcca tggccggggc
     4081 caaacaccgt cacgccgtcg gcgcgcagct gggcgatatt gcgctgattg ggcgggttgt
     4141 cccacatctg tttgttcatg gccggcgcca ccgccagcgg gcaggtgcgc gcagcggcca
     4201 gggtggtgat caggtcgtta cagtggccat gcgccagccg cgccagcaca tcggcagtgg
     4261 ccggcgcaat cagaaagcaa tcggcccggc gggtcagctc gatatgcgcc atattgttgt
     4321 cagggcgtgc atcccacaaa tccacataca ccggccggcc agacagcgcc tggaaggtga
     4381 ccggggtgac aaagcgggtg gcggattcgc tcatcgccac gtccacggtg tggcccgcct
     4441 tgaccagcag gcggacgagt tcggcggatt tataggcggc cacgccgccc gtgatgccaa
     4501 gcaggatgtg ctgagcggtc atggtcttgt cttatcaatc nnnnnnnnnn nnnnnnnnnn
     4561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4621 nnnnnnnnnn nnnnnnnnnn gcgacattgc tggtcgcatc agcgacatca cactgtagtt
     4681 tgtctgggta tgctccgaaa cagggtgcta tcaccagaaa gttgccgaaa tcaccgagcg
     4741 cccgcttggt gtgaggctgg tgcgacacag tgtgccaagc cttcaggcgg attcgcgctg
     4801 agtgtgcata ggcagagctt cacaacgatt catcgtttgc tatatttatc taggtagttt
     4861 tacgaattga caagcgcaac atataaatga tatgcactgt ttggccgctt caatccgcac
     4921 accctaccgg gtggatggcg gttgccccct aaataaaaaa gcgcaccaac agtgcgcacg
     4981 agaaagcgac tcatcatgat caattcactg cgccatcgcc tggtgttttt tgtattgctg
     5041 gtggtgactg tattggccgc cgtgttgacc acggtggcct atcagcacac ctatgccgcc
     5101 gccgaagctg gcgtgcggca aaatattgcc gccaccaccg ccaacaaggt ggccttcatc
     5161 aacgagtggg tgcattcgcg ccagcgcgtg gtcggctcgg tgctgccgcg ctttggccat
     5221 ggcgagctca agccggtgct ggatcaggcg ctggaagccg gtggttttga cgatatgtat
     5281 gtcggccagc cggacaagac catgacccag ttcagcaaag ccaccctggt gccggccggc
     5341 tacgacccca ccgtgcggcc ctggtatgtg gccgctgccg ccaccgaagg cccgattgcc
     5401 tcgccgccgt atattgacgc ctccaccaag caaccgatca tcacctttgc ccagggcctg
     5461 cgccgcaatg gccagttggt ggcggtggcc ggcggcgatg tcaccctcaa gcgcgtggtg
     5521 gaagaagtca ccgccgccca actgccgggc aacggctatg cctttctgat cacccaggat
     5581 ggcgcggtga ttgcccaccc ggcggcggat tcggcgctga aaaaaatcag cgaggtggtg
     5641 cccggcctga ccccggacag cgtgccgcgc gatggcgcca tccacaccac cacactgaat
     5701 ggcgtgaaat atttcatggt gctgcaaccc gtgggcaaaa ccggctggct gatgggcgcg
     5761 ctggtgccgc atgacgccgc gctggcgccg gtgcagcata tgctctacac cctcttgggc
     5821 gtgctggccg cctgtatcgc cctggcctcg gtggccgcct ggctgggcat tgcccaactg
     5881 atgcgcggcc tggccagcat gcgcgacgcc atgcgcagcg tggccagcgg cagcggcgac
     5941 ctgaccctgc gcctgccggt gaagggcaac gatgagctgg cggaaatcgc ccaggcgttt
     6001 aatcagtttg ttgccgcctt gcagcagatg ttcacccagg tgcgccaata cgccgaatcg
     6061 ctggccgaca ccgccgaaca gctggaccgc accgccggcc agattgccga ggattcgcgc
     6121 agtcaggcca gcgagctggc caacactgcc gccaccatcg agcaggtgac ggtcagcatc
     6181 aaccatattg ccgagcacgt gggcgaaacc gaagcactgg tcacccgctc gcgccagggc
     6241 tcgatcagct cgcaagacgg catggaagct gtcgcccagg aaatgcacgc cgtggtgggc
     6301 gccattgcct cgctacgctc ggtgatggat gggctgtcca gcaagtcgga agaaattcgc
     6361 ggcatcgtca acaccattca tgacattgcc gaccagacca atctgctggc gctgaacgcc
     6421 gccatcgaag ccgcccgcgc gggcgagcaa ggccgtggct ttgctgtggt ggcggacgaa
     6481 gtgcgcaagc tggccgaacg cacctcgacg gccaccgtgc agattgccag catcatggat
     6541 caggtgatcg accagaccgg cgacgccgcc gagcatgtgg gctccaccca gcaaagggtg
     6601 acgcaaagca tgcgccacac cgaagaagcc gccggtcggg tggcgcgcgt gcgcgagcac
     6661 accgacgaca tcgcccagcg catggccaat atcaagatgg ccaccgccga gcaaggcagc
     6721 gccacccacg atatggcgcg cagcgccgag cgggtgaacg ccaaaaccca gcaaaccgac
     6781 ggcaatctgc agcaggtgct gggcacgctg cacggcctgg cgcagtgcgg tgaatcgctc
     6841 aaggcattgg tgtcgcgctt caagctctga ggcggttctc accgacgcaa aaaagccgat
     6901 gcagatgcat cggctttttt gtggtctgac acttggcgct gaggcttatt ccaccttggc
     6961 ttgctgcttg agcttgctca ggtaagcgtc cagttgtgcg ccctggatgc gctgcgccag
     7021 ttgcggcttg agctcgtcaa acgacgggcc ttgctgggtg cgggcatcca ccagctggat
     7081 gatgtgccag ccaaactggg ttttcaccgg gttggcggta acctggcccg gggtgagctt
     7141 ggtcagcgct tcgctgaatt ccttcacata ggcttcggcc ggggcccagc ccagttcgcc
     7201 gcccttgtcc ttgctgccgg tgtccttgga aacttccttg gcgatggcgg tgaaagcctt
     7261 gccttttttc aggtcttcca ggattttgcg cgcgtcggct tcgttttcca ccaggatgtg
     7321 gcgggcgctg tattccttgc gggccagctg ggccaccagc ttgtcgtatt cagcgcgcag
     7381 ttgggcgtcg ctgaccgggt tggcgcgcat ccagtcgcgc acgtagaagt tggccagcag
     7441 acgggcggcg taggcgtcca tctcggactt gtattcagcc gacttgtcca ggcccttttt
     7501 ctgggcttcc tggcgcagca gctcggtcag caccagctga tcacgcacca tggcgcgcag
     7561 ctgcggggag tcttgctggc cttgctgttg cagttgttgc accatggcgt caacgcgcgc
     7621 ctgggacacg gcctggccat tgaccgaaaa cgccggcttg tcggccatgg ccggcaaggt
     7681 cagcaccgcg gcgatcaggc cgcacaaaag cttggtacgc atgaaatcgg tttcctgaat
     7741 gggttactga atcttggcct tgttgcgcag ttcgaccagg gttttttcga tctgggcgtt
     7801 ttgcatctgg cgggccagtt gctgcttggc ttcgtcaaag ctcggcactt tcacgtcgcg
     7861 cgcttcttcg atcttgaaca catgaaagcc cagctcggtc ttgaacggct gtgccgaaat
     7921 ctggcccttg gcgatggatt tcagctcgtc ggccagcggc ttgggcatca tgcccagatt
     7981 gccccagccc atgtcgccgc cggtttgctt ggcggccggg tctttggatt tggcgcgggc
     8041 gacatcttca aacttgccgc cttttttcag ctgggcgatg acttgcttgg cttcttcttc
     8101 gctgcccagc acgatctggc gggcgtgcac ttctttctgg cccacggttt gcgctttcag
     8161 gcggtcgtac tcggccttca cctgggcgtc ggtcactggg ttggcgcggg cgatgtcttc
     8221 aaacagagcc tgttgcaaga ggtcgttgcg gatgtcttcc aggcgatctt tgaactgggc
     8281 gctcttgtcc aggctgcgac ggctggcttc ttgcaggatg atctggcggt tgatcagctg
     8341 ttgcttgagc tcggcgcgca gctccgggct gtccttcaca cggccattgc tgcccttgac
     8401 caccgagtcg gccagttgat ccagctcgct ttgcggaatg gacgcgccgt tgaccacggc
     8461 cacggtcttg tctgctgctt gtgcgccagc ggccagcgac aaagtcagca ggatggcggc
     8521 gatgtggttc aaacgcatga tgaatactcc aggatgaata aggtcggcgc gctgcttaca
     8581 gctcgcccgg cgcataagcg cgcaggctca gcgcatgaat gcgggaagga atcaggtcac
     8641 ccagcgcctg atacaccagg cggtggcggg ctacggcatt gacgccggca aagaccggcg
     8701 acaccagggt gagttcaaaa tgaccgccgc cgctggcggc gccagcatgg ccggcgtgtg
     8761 cggcgctgtc gtcgtgcagg tggatttcct gcggggtcag cgcggccagg cgctgatgaa
     8821 tcagtgcgtc cagcgacaag gcttcgctca cgtcagaaca cctgcttgaa cggcaggatg
     8881 tccacgcggg cgtacacgcc agcggccaca tacgggtcgg cgtcggccca ggcgcgcgca
     8941 tcctgcagcg aatcaaagtc agcgacaatc acgctgccca caaaaccggc cgggccgggg
     9001 tcagcgctgt cgatggccgg gcaggggccg gccagggtca ggcgaccggc gtcgcgcaga
     9061 gcggtcagcc gggccaggtg ttccgggcgg gcggccagac gttgctcgag agaattcggc
     9121 acatcgtggc cgatgatgac gtaacgcatc agtcagagtc ttccatatag cgggcgagca
     9181 cgacgctttg tccaagcacg aacaacaaca tcaggcccat gccgccaaac agtttgaaat
     9241 tgacccacac ttcctcgctg aaagtgaagg cgacgtataa gttaagcgcg cccatcccgg
     9301 caaaaaacgc cacccacgcc agactcaggc gccgccagac agcttctggc agggtgagct
     9361 gcttgcccat cagggatttc agcggctgct tgcccatggc ctggctgacc agcaagccca
     9421 cgcccatcag ccagtacagc acggtgggct tccacatgat gaaggacttg tcgtgcagca
     9481 agagcgtggc gccgcccagc acgacaatca gcccgagcga cacccattgc atggtgtcaa
     9541 ccttgcgatg cttgaaccag ctgaacgcca cttggccaac cgtggccgcc atggccaccg
     9601 ccgtggcgag gtagatgtcg cgggtgaggg aaaacgcggc aaaaaacagg atgaccggaa
     9661 acaggtcgaa gagaaatttc atgcggctga ttatgcagcc aagctgggtg gctggcaagc
     9721 cgaccactgg cgggcttgac gctcagccca tggccgtggc gcaggcgcga cagtgggcgt
     9781 cgcgcc
//
LOCUS       DAOPYX010000092         9776 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_8162_length_9776_cov_11.643144, whole genome shotgun sequence.
ACCESSION   DAOPYX010000092 DAOPYX010000000
VERSION     DAOPYX010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 9776)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9776)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9776
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8162_length_9776_cov_11.643144"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            259..1260
                     /locus_tag="PKN55_06300"
     CDS             259..1260
                     /locus_tag="PKN55_06300"
                     /inference="COORDINATES: protein motif:HMM:NF012407.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HNX70886.1"
                     /translation="MKLCLIPGDGIGREVVPVAAAALQRLLPAVQLQQADAGWECFVR
                     TGQALPDATLARARECGAVLFGAVSSPARKVEGYRSPIVALRRELNAYANLRPTCALP
                     IAGARQDVDMLIVRENTEDLYIGEEQRDGDTAIAIKRITAAASRRVARAACETLLARG
                     MHRLVIVHKANIMPLTDGLFRDCCREVAQSYPQIEVSELLVDTAAYQLARAPQQFQAL
                     VAPNLYGDILSDLAAAHGGGLGLAASLNQGDAGVAIAEPVHGSAPDIAGQGVANPVAA
                     LLSVALLLRHTWQRPDEAQRLEHAVRDTLAAGIATPDVAGPAGVSTAAFAQALLARL"
     gene            complement(1334..>2713)
                     /locus_tag="PKN55_06305"
     CDS             complement(1334..>2713)
                     /locus_tag="PKN55_06305"
                     /inference="COORDINATES: protein motif:HMM:NF023334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3426 domain-containing protein"
                     /protein_id="HNX70887.1"
                     /translation="FAMYITQCPNCQTRFKVQPGQLDVCDGQVRCGRCATVFDARASL
                     EPVNEPAPAKPAAAAPSAPAALADDNLPAEAGHLVRALANAPQNGQWSAPSPQDEATA
                     IPLPADGDLDRISKNIDLELPDFAADAGSAGKAVRQEPVLRPDPLPKPVATAAAKPAS
                     PPPAARPAAKQPEVIPDDEGEDDTFLKKLEAARHQQTAPADKPAPAKADTPPAGGKRI
                     EPSFKAEVLPDDEDDGVVYKGNPLAADPTVEPAPFIAHSAELLKGGAPATEDKKGFAL
                     PKVTLPKLPAFSLPALPEFSFSAGGKLVWLWTAAAVVAAIVLLVQVVYVYRTPIAAEL
                     PGSRGVLESMCKGLGCDVPLPRRSDQLRTEYSELAAVPDQANLIRVSATVRNQAAYPQ
                     AYPHLELTLKDGEDRVLSRKVFAPKDYLSKNDFALGQFNGNSEIKVNMQLDIGLLRAS
                     GYSLMWFYP"
     assembly_gap    2714..2813
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2814..3701)
                     /gene="prmA"
                     /locus_tag="PKN55_06310"
     CDS             complement(2814..3701)
                     /gene="prmA"
                     /locus_tag="PKN55_06310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003684134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HNX70888.1"
                     /translation="MSWLQVTLDTDSAVAEKLADALMDLGALSAAIEDAAAGTDAEEP
                     IFGEPGEPVDRMWRTSRILALFDPAQDVALLIAAAASECGVPTPSWQMEEIAEQDWVR
                     ATQAQFDPIRISDRLWITPTWHEAPNPDAINLQLDPGLAFGTGSHPTTRLCLQWLDRE
                     LQAGESVLDYGCGSGILAIAALKLGAGRALGVDIDPQAVQSSRDNAAQNAVQADFYLP
                     GELPDVETDVVVANILANPLRMLGALLTRHVRVGGRIVLSGILQEQAEEIRTLYQQWF
                     EMDAPVFDDGWTCLSGRKR"
     gene            complement(3698..5056)
                     /gene="accC"
                     /locus_tag="PKN55_06315"
     CDS             complement(3698..5056)
                     /gene="accC"
                     /locus_tag="PKN55_06315"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008954854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HNX70889.1"
                     /translation="MFEKILIANRGEIALRIQRACREMGIKTVVVHSEADREAKYVKL
                     ADESVCIGPAASALSYLNVPALISAAEVTDAQAIHPGYGFLSENADFAERVEQSGFVF
                     IGPRPDTIRLMGDKVSAKNAMIASGVPCVPGSEGALPDDPAQIAKIAKKIGYPVIIKA
                     AGGGGGRGMRVVHTEGALINSVQMTRTEAGAAFGNPTVYMEKYLEQPRHIEIQVLADE
                     HGNAIYLGERDCSMQRRHQKIIEEAPAPGITDKQRKQIGEACAAACRKIGYRGAGTFE
                     FLFENNEFYFIEMNTRVQVEHPVTELITGIDIVQEQIRVAAGQKLRYTQKQVQLKGHS
                     MECRINAEDPFTFVPSPGRIDGYHPPGGPGIRVDSHIYHGYTVPPYYDSMIAKLIAYG
                     DTREQAMARLRVALSEMAIGGIKTNIPLHQELLVDSAFIQGGTSIHYLEARLAKLKKS
                     EA"
     gene            complement(5211..5663)
                     /gene="accB"
                     /locus_tag="PKN55_06320"
     CDS             complement(5211..5663)
                     /gene="accB"
                     /locus_tag="PKN55_06320"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003766308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HNX70890.1"
                     /translation="MDLRKLKKLIDLVEESGIAELEVTEGEEKVRITRVQAGAAMAYP
                     AMTQVALPQAAPAAAPAAAAPAEAAKPSAQNAVKSPMVGSFYRSATPGGKPFAEIGQS
                     VSVGDTLCIIEAMKLMNEIESDRAGVIKAILVENGEPVEFGEPLFVIE"
     gene            complement(5722..6204)
                     /gene="aroQ"
                     /locus_tag="PKN55_06325"
     CDS             complement(5722..6204)
                     /gene="aroQ"
                     /locus_tag="PKN55_06325"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011134542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HNX70891.1"
                     /translation="MMTNPEQKAAAPAPYVLALHGPNLNMLGMREPHLYGADTLADIN
                     GRLAAQAQAAGMGFDWLQSNAEHVLIDRIHAAYTDGAAFIIINPAAFTHTSVALRDAL
                     AAVKIPFIEVHLSNVHAREAFRQHSYFSDLAVGVICGLGAKGYEYALSHALNHLHARA
                     "
     gene            6352..7380
                     /gene="queA"
                     /locus_tag="PKN55_06330"
     CDS             6352..7380
                     /gene="queA"
                     /locus_tag="PKN55_06330"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014088263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HNX70892.1"
                     /translation="MQLSDFDFELPEALIAQFPPATRGDSRLLHVDGLCCTDDHFSQL
                     SAYLRAGDVLVFNDTRVLKARLFGQKASGGQVEILVERVLDAHRVLAHVRASKSPKPG
                     SRLLLEGRWQAEMVERQEALFLLRFLDEANVLDILEQSGKLPLPPYITRDADSPDDER
                     YQTVYARHQGAVAAPTAGLHFTDTQLDALRAQGVKLAFVTLHVGAGTFQPVRVDNIAE
                     HHMHSERYDIPPATVEAIAQAHANGGRVAAVGTTSLRALESAARGGELAAGQGETDIF
                     ITPGYRFRVVDRLLTNFHLPKSTLMMLVSAFAGMAPIRAAYRHAIEQQYRFFSYGDAM
                     LLEKSPDA"
     gene            7408..7782
                     /locus_tag="PKN55_06335"
     CDS             7408..7782
                     /locus_tag="PKN55_06335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014088262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF971 domain-containing protein"
                     /protein_id="HNX70893.1"
                     /translation="MSGLSPDTPQPTEIRLHQQSRALELHFDDGAQFTLPCEYLRVYS
                     PSASVRGHGPGQEVLQTGKRLVNIVNIEPVGHYAVKLVFSDGHDSGLYSWDYLYQLGR
                     EQEARWAEYLARLAAAGASRDE"
     gene            8092..8829
                     /gene="ubiE"
                     /locus_tag="PKN55_06340"
     CDS             8092..8829
                     /gene="ubiE"
                     /locus_tag="PKN55_06340"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HNX70894.1"
                     /translation="MMAEQTHFGFQTVDAAQKAGKVAEVFHSVANKYDVMNDFMSAGM
                     HRVWKRFTLDTAGVRPGQKVLDIAGGTGDLARGWAKRVGRSGEVWLTDINSSMLTVGR
                     DRLADEGVLLPVALADAEKLPFPSNYFDLVSVAFGLRNMTHKEGALAEMLRVLKPGGK
                     LLVLEFSQVWGPLKPAYDLYSFKALPLMGKLVANDAESYRYLAESIRMHPDQDTLKQM
                     MLDVGFGKVDYHNLSAGVVALHKGWKF"
     gene            8876..9472
                     /locus_tag="PKN55_06345"
     CDS             8876..9472
                     /locus_tag="PKN55_06345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008954861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sterol-binding protein"
                     /protein_id="HNX70895.1"
                     /translation="MNRPQLAVLNHLLAQQEGVRQRLAEHAGRRFRVELPPLGVSAVV
                     LADGFAGQTDGEPEARVRLSSSLLVKRLTGGEPGAGDVQLDGDAELGLALARILADLR
                     WDAVEDLSRVVGDVAAYRAERWLRGALGVKGEVAWRLASAYAEHLKEETPLLCKRTQV
                     DAWHGQVDALRDDVARADKRLARLEALLAARLPAPTAE"
BASE COUNT         1604 a         2945 c         3326 g         1801 t
ORIGIN      
        1 tgctgcatca ttggggtgtg gctgggcgcg gcgctgtcgg cggagggttt tgtcatggca
       61 atcagaggct tgaatccggc gcacaggccg gcgatggcgg attgtatgcg ggaatgccgt
      121 cggcgtgcca tagccggctg ccgattgccc gccgggtggc attccgctgt gcaatccagc
      181 cgcccattct gcttgggcgg ctggcggcgg ctgccgtaca ctgccgggca tcttgcgtgt
      241 agatgacggt gaatgacgat gaaactgtgt ctgatccccg gcgatggcat tggccgggaa
      301 gtggtccctg tggcggcggc ggcgctgcaa cggctgttgc cggcggtgca gctacagcag
      361 gcggatgccg gttgggaatg ttttgtgcgc accggccagg ccctgccgga cgccaccctg
      421 gcgcgtgcgc gtgaatgcgg cgcggtgttg tttggcgcgg tcagctcgcc ggcgcgcaag
      481 gtggagggct atcgctcgcc gattgtcgcc ctgcgccgcg agctgaacgc ctacgccaat
      541 ctgcgcccaa cctgcgcctt gccgattgcc ggcgcgcgcc aggatgtgga tatgctgatc
      601 gtgcgggaaa acaccgaaga tttgtatatc ggcgaagaac agcgcgacgg cgacaccgcc
      661 atcgccatca agcgcatcac cgccgccgcc tcgcgccggg tggcgcgcgc cgcctgcgaa
      721 accttgctgg cgcgcggcat gcaccggctg gtgattgtgc acaaggccaa tatcatgccg
      781 ctgaccgatg ggctgtttcg cgattgctgc cgcgaagtgg cgcaaagtta tccacagatc
      841 gaggtcagcg agctgttggt ggacaccgcc gcctaccagc tggcgcgcgc gccgcagcag
      901 tttcaggcgc tggtggcgcc caatttgtat ggcgatattt tgtctgatct ggccgctgcc
      961 cacggcggcg gcttgggcct ggccgcctcg ctgaaccagg gcgacgcggg cgtggcgatt
     1021 gccgagccgg tgcatggctc ggcgccggac atcgccggcc agggcgtggc caatccggtg
     1081 gcggcgctgc tgagcgtggc cttgctgctg cgccacacct ggcagcgccc ggacgaagcg
     1141 cagcggctgg agcacgccgt gcgcgacacc ctggccgccg gcatcgccac cccggatgtg
     1201 gccggcccgg ccggggtgag cacggcggcg tttgcccagg cgctgctggc gcgtttgtaa
     1261 aaaacccaca aaaaaccgcc cgggcgctgc ggcgcacggg cggtttcggt ttggccaaac
     1321 acgcgcaaat tgattacgga taaaaccaca tcaggctgta accactggca cgcagcaggc
     1381 cgatgtccag ctgcatattc accttgatct cgctattgcc gttgaactgg cccagggcaa
     1441 agtcgttttt gctcaggtag tctttggggg caaacacctt gcgcgacagc acgcggtctt
     1501 cgccgtcctt cagcgtcagt tccagatgcg ggtaagcttg cgggtaggca gcctgattac
     1561 gcacggtggc agacacacgg atcagattgg cttgatccgg cacggcggcc agctcggagt
     1621 attccgtgcg cagctggtcg ctgcggcgcg gcagcggcac atcgcaaccc aagcccttgc
     1681 acatcgattc cagcacaccg cgtgagcctg gcagctcggc ggcaatcggg gtgcggtaga
     1741 cgtacaccac ctgcaccagc agcacaatgg ccgccaccac ggcggcggcg gtccacagcc
     1801 acaccagttt gccgccagca gagaaagaaa actcgggcag agccggcaag gaaaacgctg
     1861 gcagcttggg cagcgtcacc ttgggcaggg caaagccttt tttgtcttct gtcgcaggcg
     1921 cgccgccttt cagcaactcg gcgctgtggg cgatgaaggg cgcaggctcc acggtggggt
     1981 cggccgccag cggattgccc ttgtacacca cgccgtcgtc ttcgtcgtcg ggcagcactt
     2041 cggccttgaa gctgggctcg atgcgcttgc cgccagctgg cggcgtgtcg gccttggccg
     2101 gagcgggctt gtcagcgggg gcggtttgct gatggcgggc ggcttccagt tttttcagga
     2161 aggtatcgtc ttcaccttca tcatccggga tgacttccgg ctgcttggca gcgggcctgg
     2221 ccgccggcgg cgggctggcc ggcttggccg cagcggtggc caccggcttg ggcaacgggt
     2281 cgggacgtag tacgggctcc tgacggacag ccttgccggc cgagccagcg tcggcggcaa
     2341 aatccggtag ttccagatca atattcttgc tgatgcggtc cagatcccca tctgccggca
     2401 gcggaatggc ggtggcttca tcctgcgggg agggcgcact ccattggcca ttttgcggcg
     2461 cattggccag cgcgcgcacc agatgaccgg cctcggcggg caggttgtca tcagccagag
     2521 cggcgggcgc agagggggca gcggcggcgg gcttggccgg ggccggctcg ttgaccggct
     2581 cgagcgaggc acgggcatcg aacacggtgg cgcagcggcc gcagcgaacc tggccgtcgc
     2641 agacgtcaag ctgacccggc tggaccttga agcgggtctg gcagttcggg cattgcgtga
     2701 tgtacatggc gaannnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnttagcgt
     2821 ttgcgcccgg acaaacaggt ccagccgtcg tcaaacaccg gcgcatccat ctcaaaccat
     2881 tgctgataca gcgtgcggat ttcttccgcc tgctcttgca gaatgccaga cagcacgatg
     2941 cggccgccca cgcgcacatg gcgggtgagc aaggcgccca gcatgcgcag cgggttggcc
     3001 aggatattgg ccaccaccac atcggtttcg acgtcgggca gctcgcccgg caggtagaaa
     3061 tctgcctgca cggcgttttg cgcggcgttg tcgcggctgg actgcaccgc ttgcgggtcg
     3121 atgtccacgc ccagcgcccg gccggcgcca agcttgagcg cggcgatggc cagaatgccc
     3181 gagccgcaac cgtagtccag caccgattcg ccagcttgca gctcgcggtc cagccattgc
     3241 aggcacagcc gggtagtcgg gtggctgccg gtgccaaacg ccaggcccgg atccagttgc
     3301 agattgatgg cgtccgggtt gggcgcttcg tgccaggtgg gggtgatcca cagccggtca
     3361 gaaatgcgga tcgggtcgaa ctgcgcctgg gtggcgcgca cccagtcctg ctcggcgatt
     3421 tcttccatct gccagctggg ggtgggcacg ccgcactcgc tggcggcggc ggcgatcagc
     3481 agcgccacgt cttgcgccgg gtcgaacagc gccaggatgc ggctggtgcg ccacatgcgg
     3541 tccaccggct cgccgggttc gccaaaaatc ggctcttcgg cgtcggtgcc ggcggcggcg
     3601 tcttcaatcg ccgccgacag cgcgcccagg tccatcagcg cgtcggcgag tttttccgcc
     3661 acggcggaat cggtgtctag ggtgacttgc agccagctca tgcttcggat ttcttcagtt
     3721 tggccaggcg tgcttccaga tagtggatgg aggtgccgcc ctggataaag gcggagtcca
     3781 ccagcagttc ctggtgcagc gggatgttgg tcttgatgcc gccgatggcc atttccgaca
     3841 gggccacgcg caggcgcgcc atggcctgtt cgcgggtgtc gccgtaggcg atcagcttgg
     3901 cgatcatcga atcatagtac ggcggcacgg tgtagccatg gtaaatgtgc gaatccacgc
     3961 gaatgccagg gccgccgggc gggtggtagc cgtcgatgcg gccagggctg ggcacaaagg
     4021 tgaacggatc ttcagcgttg atccggcatt ccatggagtg gcccttgagc tgcacctgct
     4081 tttgcgtgta gcgcagtttt tggccggccg cgacgcggat ttgctcttgc acgatgtcga
     4141 tgccggtgat cagctcagtc accgggtgct ccacctgcac gcgggtgttc atctcgataa
     4201 agtagaattc gttgttttca aacaagaatt caaacgtgcc ggcgccacgg tagccaatct
     4261 tgcggcaggc ggcggcgcag gcttcgccaa tctgcttgcg ctgcttgtcg gtgatgcccg
     4321 gcgccggcgc ttcttcgatg attttctggt ggcggcgctg catcgagcaa tcgcgctcgc
     4381 ccaggtaaat ggcgttgccg tgttcgtcgg ccagcacctg gatttcaatg tggcgcggtt
     4441 gctccaggta tttttccatg tacacggtgg ggttgccaaa agcggcgccg gcttcggtgc
     4501 gggtcatttg caccgagttg atcagcgcgc cttcggtgtg caccacgcgc atgccgcgcc
     4561 cgccgccgcc gccggcggct ttgatgatca ccgggtagcc gatttttttg gcgattttgg
     4621 cgatttgcgc cggatcatcc ggcagcgcgc cttccgagcc gggcacgcag ggcacgccgg
     4681 aggcgatcat ggcgtttttg gcgctgacct tgtcgcccat caggcgaatg gtgtccgggc
     4741 gcgggccgat gaacacaaag ccggattgct cgacgcgctc ggcaaagtcg gcgttttccg
     4801 acagaaaacc ataacccggg tgaatcgcct gggcgtcggt gacttcggcg gccgagatca
     4861 gcgccggcac attcagatag ctcagcgcag acgcagccgg gccaatgcac accgattcgt
     4921 cggccagctt gacgtatttg gcttcgcggt cggcctcgga gtgcaccacc acggttttga
     4981 tgcccatttc acggcaggcg cgctggatgc gcagggcgat ttcgccacgg ttggcgataa
     5041 ggattttttc aaacatgggg attcctgaac agcgtccggc acggtcaagg aacgggcggc
     5101 gccagcggcg gggaagggcg gcaagggacg catagagtcg ccgcccggcc gctggggcgt
     5161 ccggcggcgc acgatcgggg cacacaagcc cgatgcgcgc cggccgggga ttattcgatg
     5221 acgaacagcg gctcgccgaa ttccaccggc tcgccgtttt ccaccagaat cgccttgatc
     5281 acgccggcgc ggtcggattc gatttcgttc atcagcttca tggcttcgat gatgcacagg
     5341 gtgtcgccca cgctcacgct ctggccgatt tcggcaaacg gcttgccgcc cggcgtggcc
     5401 gaacggtaga aggagcccac catcggcgac ttcacggcat tctgcgcgct gggcttggcg
     5461 gcttcagccg gcgcagcagc ggccggcgcg gcggccgggg cggcttgcgg cagcgccacc
     5521 tgggtcatgg cggggtaggc catggcggcg ccagcctgca cgcgggtgat gcgaactttt
     5581 tcctcgcctt cggtcacttc cagttcggca atgccggact cttccaccag gtcgatcagt
     5641 tttttcagct tgcgaagatc catgggtttc ctttttgacg tcgagccgcc ccgcctcgta
     5701 tcaaggcttg ggcgggcggg ttcaggctcg agcgtggaga tgattcaatg catgggacaa
     5761 ggcgtattca tagcccttgg cgcccaggcc gcagatgacg ccgacggcga gatccgagaa
     5821 gtaggaatgc tgacggaacg cttcccgggc gtgaacattg gacagatgca cttcgatgaa
     5881 ggggattttc accgccgcca gggcatcgcg cagcgccacg ctggtgtggg tgaaggcagc
     5941 cgggttgatg atgatgaacg cagcgccatc ggtgtaggcg gcgtggatgc ggtcgatcag
     6001 cacatgttcg gcgttgcttt gcagccagtc aaagcccatg ccagcggctt gagcctgggc
     6061 agccaggcgg ccgttgatgt cggccagggt gtcagcgcca tacagatgcg gttcgcgcat
     6121 gcccaacatg ttcaggttgg ggccatgcag cgccagcaca tagggggcgg gcgcggcagc
     6181 tttttgctcg ggattcgtca tcatgggcgc gagtttgccg catttggccg aaaaaagtcc
     6241 agcttttccc gaagttcaaa catttgtttt aaagcaatgg cgccgtgggg aaggccgcga
     6301 aaaggccatg aactgctata ctggccaaca ttgtgcctga ctgtattttt catgcaactg
     6361 tccgattttg attttgaact gcccgaggcg ctgattgccc agtttccacc ggccacgcgc
     6421 ggcgacagcc gtttgctgca cgtcgatggt ctctgctgca cggatgacca tttcagccag
     6481 ctgagcgcct atctgcgcgc cggcgacgtg ctggtattca acgacactcg cgtgcttaag
     6541 gcccgcctgt ttggtcagaa ggccagcgga ggacaggtgg aaattttggt cgagcgcgtg
     6601 ctcgacgccc atcgggtgct ggcgcatgtg cgcgccagca agtcgcccaa gccgggttcg
     6661 cgcctgctgc tggaaggccg ctggcaggcc gagatggttg aacgccagga ggcgctgttc
     6721 ctgctgcgct ttctggacga ggccaatgtg ctggatattc tggagcaatc cggcaagctg
     6781 ccgctgccgc cctatatcac ccgcgacgcc gattcacccg acgacgagcg ctatcaaacc
     6841 gtctacgccc gccatcaggg cgcggtggcg gcgcccaccg ccggcctgca ctttaccgac
     6901 acccaactgg acgcgctgcg cgcccagggc gtgaagctgg cgtttgtcac cctgcatgtg
     6961 ggcgccggca cgtttcagcc ggtgcgggtg gacaatatcg ccgagcacca catgcacagc
     7021 gagcggtacg acatcccgcc cgccaccgtc gaggcaattg cccaggcgca cgccaacggc
     7081 ggccgggtgg cagcggtggg caccaccagc ctgcgcgcgc tggaatccgc cgcgcgcggc
     7141 ggcgagctgg ccgccggcca gggcgagacc gatattttca tcaccccggg ctatcgcttt
     7201 cgcgtggtgg accggctgct gaccaatttt cacctgccca aatccacctt gatgatgctg
     7261 gtgtcggcgt ttgccggcat ggcgccgatc cgcgccgcct atcgccacgc cattgaacag
     7321 caatatcgct tcttcagcta tggcgacgcc atgctgctgg agaaatcgcc cgacgcctga
     7381 cgcgccggcc atgcacagga gcgcaccatg agcggacttt cccccgacac cccgcaaccg
     7441 actgaaatcc gcctgcatca gcagtcgcgg gcgctggagc tgcattttga cgatggcgcg
     7501 caattcaccc tgccgtgtga gtatctgcgg gtgtattcgc catcggcctc ggtgcgcggc
     7561 cacggcccgg ggcaggaggt gctgcaaacc ggcaagcggc tggtgaatat cgtcaatatc
     7621 gagccggtcg gccattacgc ggtgaagctg gtgttctcag acgggcatga ttcgggtttg
     7681 tacagctggg attatttgta tcaactgggg cgagagcagg aagcgcgctg ggcggagtat
     7741 ctggcgcggc tggcggcggc aggggcgagc cgggacgagt aaggcggggc ttcgccccac
     7801 acccgccccc tttttctttg tctcgccaaa gaaaaagggg ggaaagcaag gcgacccggg
     7861 acgaaagggt aaccggctaa gagccgctaa caaaaccctc ctggcgtcgt tgcacggcct
     7921 tgccgtactc cttgtactgt ctgcggccgc gcgcctagcc aggagcgctt cgctgagttt
     7981 tgttagcggc tctaagcaca accggcggag catgaaatta aggcctggtg gatggggcaa
     8041 gagacaatta gatgccgcca ggccgatctt attgaaaatg acgggataac aatcatggca
     8101 gaacaaacgc atttcggttt ccagacggtg gatgccgcgc agaaggcggg caaggtggct
     8161 gaggtgtttc actcggtggc caataaatac gatgtgatga acgatttcat gtctgccggc
     8221 atgcaccgtg tgtggaagcg cttcaccctg gacaccgccg gggtgcggcc ggggcagaag
     8281 gtgctggata ttgccggcgg caccggggat ctggcgcgcg gctgggccaa gcgggtgggg
     8341 cgcagtggcg aggtgtggtt gacggacatc aacagctcga tgctgaccgt gggccgcgac
     8401 cgtctggccg atgagggggt gttgttgccg gtggccttgg ccgatgccga gaagctgccc
     8461 ttccccagca attattttga tctggtgtcg gtggcgtttg gcttgcgcaa tatgacccac
     8521 aaagagggcg cgctggcgga gatgctgcgc gtgctcaagc cgggcggcaa gttgctggtg
     8581 ctggagtttt cccaggtgtg ggggccgttg aagccggcgt atgatctgta ttcgttcaaa
     8641 gccttgccgc tgatgggcaa gctggtggcc aacgatgccg agagctaccg ctatctggcc
     8701 gagtccatcc gcatgcaccc ggaccaggac accctcaagc agatgatgct ggacgtgggg
     8761 tttggcaagg tggattacca caacctcagc gccggagtgg tggcgctgca caagggctgg
     8821 aaattctgac ccccgccggc gcgcgcgccg ggcggtcttt tttgcgaggt ttgccatgaa
     8881 tcgcccgcaa ctggctgtgt tgaatcattt gctggcccag caagagggcg tgcgccaacg
     8941 cttggcggag cacgcgggcc ggcgctttcg ggtggagttg ccgccgctgg gcgtcagcgc
     9001 cgtggtgttg gcggatggct ttgctggcca gaccgatggc gagccagaag cccgcgtgcg
     9061 cttgtccagc tcgctgctgg tcaagcgcct gaccggcggc gagccgggcg ccggcgatgt
     9121 gcagctggac ggcgacgccg agctgggcct ggcgctggcg cgcattctgg ccgacttgcg
     9181 ctgggacgcg gtggaagact tgtcgcgggt ggtcggcgat gtggccgcct accgcgccga
     9241 gcgctggctg cgcggcgcgc tgggggtgaa gggcgaggtg gcctggcgac tggccagcgc
     9301 ctacgccgaa cacctgaaag aagaaacccc cttgctgtgc aagcgcaccc aggtggacgc
     9361 ctggcatggc caggtggacg ccctgcgcga cgacgtggcg cgcgccgaca aacggctggc
     9421 gcggctggaa gccctgctgg ccgcgcgcct gccggcgccg acggccgaat aagcgccgtg
     9481 tgacctcttc ttggcgtggt gtatcgtgtg ggtgcgcgcc aagcgctgcc tggtgcgtga
     9541 aacccgcgag atgccttgcg ccaagcgtgg cgaacaccac ccagcaaagc gtttccagcg
     9601 aggtgcgcgc agacacggca aggcagcacc acaacgccaa ggaagctttg tgagcgacgc
     9661 tgagtgtgtt ggcggcgtca cagccgtgcc gccatatcga gcatctgggc gcgccgcccc
     9721 agcccaaccc cgccggccgt atgccggctt gagcctgtga tcatgcgatt ttctcg
//
LOCUS       DAOPYX010000093         9708 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_8227_length_9708_cov_13.090127, whole genome shotgun sequence.
ACCESSION   DAOPYX010000093 DAOPYX010000000
VERSION     DAOPYX010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 9708)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9708)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9708
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8227_length_9708_cov_13.090127"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(58..693)
                     /locus_tag="PKN55_06350"
     CDS             complement(58..693)
                     /locus_tag="PKN55_06350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011133743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HNX70896.1"
                     /translation="MPLSTWLIYFATVLVVSATPGPNMLLAMTHGIRFGLRRTTPTLL
                     GLLLALALIMGLSAAGLGALLVTSEWLFGLVKYAGAAYLLWLGIKTWRSQPQALAEGE
                     AEAQAGSGWAGFRTGFLVAMSNPKAIVFFTALFPQFMDPAQPQGPQLLILAATFFVIE
                     TSWQCAYAAGGARLKAWLNSPRRLKLMNRVAGGSFIAAGVALTRASSQAHA"
     gene            complement(722..1231)
                     /locus_tag="PKN55_06355"
     CDS             complement(722..1231)
                     /locus_tag="PKN55_06355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009116808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgcJ/EcaC family oxidoreductase"
                     /protein_id="HNX70897.1"
                     /translation="MIRFTRTLAAAAAAALLSTACVNVTVHSGQGHMPAMQGKHAGNE
                     AEIAALFDRWNASLATGNPDDVAANYAADAILLPTVSNKVRHNPAEIRDYFVHFLEKK
                     PKGKIVERNIRFPAPNVAIDSGLYVFDTTGGQVPARYTFVYRKVGGTWLIAEHHSSAM
                     PEKPGAKAH"
     gene            complement(1349..2527)
                     /locus_tag="PKN55_06360"
     CDS             complement(1349..2527)
                     /locus_tag="PKN55_06360"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003677098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HNX70898.1"
                     /translation="MKFKKLTDQALAGKRVLIRVDMNVPVKNGVIGDDTRIRASLASI
                     QHALYNGAAVILMTHLGRPTEGEPKPEDSLAPVAARLGELLNKPVPVVADWQAGLSLN
                     AGDVVMLENVRLNKGEKKNNEDLGRAYAALCDVFVNDAFGTAHRAEASTHAVAKFAPL
                     ACAGILLAAELDALGKALEAPARPLVAIVAGSKVSTKLTILEALADKVDQLIVGGGIA
                     NTFLLAEGKAIGKSLAEAELVGEAQKVIAKIKAHGGAVPLPSDVVVAPEFSENADDTL
                     KPVSEVTKQDMILDIGPQSAQELADIVAKAGTVVWNGPVGVFEFDQFASGTQTLAKAI
                     ADSPAFSIAGGGDTLAAIAKFGVTDKISYISTGGGAFLEFLEGKELPAVAILAERADS
                     "
     gene            complement(2624..3634)
                     /gene="gap"
                     /locus_tag="PKN55_06365"
     CDS             complement(2624..3634)
                     /gene="gap"
                     /locus_tag="PKN55_06365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014088752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HNX70899.1"
                     /translation="MSIRIAINGYGRIGRQVLRAIYENNLRNEFEVVAVNASGDLATN
                     AHLTQFDTVHGRFNAQVSHDEKSLVINGDVIPFFSTRNPAELPWGELKVDLVLECTGA
                     FTSKEKCQAHITAGAKKVLISAPGGDDVDATIVYGVNHNVLTADMTVVSNASCTTNCL
                     APVAKALNDGLGIVKGLMTTIHAFTNDQVLTDVRHKDLRRARSATQNMIPTKTGAAKA
                     VGLVLPEMNGKLDGFAVRVPTINVSLVDLTFEAGRATTVAEVNQIVKAAAEGPMKGVL
                     GYNVLPLVSMDFNHTSEASHFDSTLTKVSNGNMVKVLAWYDNEWGFSCQMLNTARAMF
                     AA"
     gene            complement(3707..5692)
                     /gene="tkt"
                     /locus_tag="PKN55_06370"
     CDS             complement(3707..5692)
                     /gene="tkt"
                     /locus_tag="PKN55_06370"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012695538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HNX70900.1"
                     /translation="MVSRSDLANAIRVLSMDAVQKANSGHPGMPMGMADIAEALWRHH
                     LKHSPANPGWFDRDRFVLSNGHGSMLLYSLLHLSGYDLSIDDLKAFRQWHSKTPGHPE
                     YRYAPGVETTTGPLGQGITNAVGMALAEKVLAAEFNRDGHAIVDHHTWVFLGDGCLME
                     GISHEACSLAGTWGLGKLIAFYDDNGISIDGDVEGWFTDDTVKRFEAYGWQVIPAIDG
                     HDFAALDAAIAAAKADTARPTLICCRTKIGKGAPNKEGGHDVHGAPLGDAEIAATRAH
                     LNWPHAPFEIPADIASAWNAREAGAAREAEWTERFAAYRAAFPAEAAEFERRMRGELP
                     ANWAAHAAAAIEKINAKGETIATRKASQNAIEAFAPALPELLGGSADLASSNLTLWSG
                     SKGVSREQGGNYVYYGVREFGMAAIMNGATLHGGFKPYGATFLMFSEYARNALRMAAL
                     MKINPIFVFTHDSIGLGEDGPTHQPVEQTATLRLIPNMDTWRPADTVESAVAWQAAIE
                     RQDGPSTLIFSRQNLPFLSRDAEQIAAIRRGGYVLREADKARAVLIATGSEVELALKA
                     QALLAEEGIAVRVVSMPSTNVFDRQDKAYIASVLPAGLPRVAVEAGVSDFWRKYVGLE
                     GGVVGLDHFGESAPAKQLFEAFGFTAANVAQTVKSVL"
     gene            complement(5889..7199)
                     /locus_tag="PKN55_06375"
     CDS             complement(5889..7199)
                     /locus_tag="PKN55_06375"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HNX70901.1"
                     /translation="MFKQTHVRNLCALALWGLAASAWADEAGKVVLSVGEVRVQGQAV
                     KAGHSVQAGDRLSTGADGYVYIKTVDNGFLILRPNSDASIIAYQADSHTPANSRFKFE
                     LREGVARSISGQAVKNARQNFRFNTPVAAIGVRGTDFTVFTDAQTTRIAVLSGGVVVS
                     GFGQGCAPEGGGPCETPHSRELFANQQRDGVLQVSRGQLAPELLRGKALSPDATTPPQ
                     PDESGKVKAGSRHNTSDANPDNTSLAPLKLAELDKASSLMPAPAPTPPGPAAIAWGRW
                     QGVAEQGANLNLADLMSKDYRVSVLTGDYALLRHQSATWQVPYGGAEFGLQGSMAQVQ
                     RDGVSQLANVENARLWVDFERSRFGTRFDLTAANLRVGLQAQGNVGSDGTFSNPSQYL
                     DGNNMVVQGVMANGALGKVFAGYTFQSRLDANTVATGVTLWGSK"
     gene            7294..8535
                     /locus_tag="PKN55_06380"
     CDS             7294..8535
                     /locus_tag="PKN55_06380"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNX70902.1"
                     /translation="MTRHGKLACRIMGLALLALAAGMVRADDGAGSDNPYLDAMQPLI
                     DGRYEEAGEALRRLVAREPEHAGAWLDLAILQCGLGNASEAELLFDAIEARFDPPPGI
                     REIIALQRAKGCHGPSARWEGRLKLGRGSDSNANQGASNPNFSIGQGSSVLNLVLSPE
                     FTPHRDRFNQITLDGAVSGLGNGWQGYAQLQSRRYDTQRRYDSESALLGAERPWRLGR
                     WDARLGASVGLTRLGGESYQRYATISGWLAPPLALPTGWQLGWLNSFSHVVYPSRSHF
                     DASLWESRAVLSLQHGPLWVQGSAGYGLDVGSHERAGGDRHGVFASLNARALLGGGWL
                     GELSWQYQNWAGAQAYSPGLIDVARRQHTQLTRATLWYELTKQQALYLEYRGVRNREN
                     ISLFEYQSRAWQLGWQWQFGR"
     gene            8739..>9708
                     /gene="hemA"
                     /locus_tag="PKN55_06385"
     CDS             8739..>9708
                     /gene="hemA"
                     /locus_tag="PKN55_06385"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HNX70903.1"
                     /translation="MQLVALGLNHHTAPLAIREQLAFPAEQLADALRDLTDSRAAHEA
                     AILSTCNRTEIYAGAENIDAVLHWLARNRGLDVDTLRPYLYLLDAEAAARHAFRVASG
                     LDSMVLGETQIVGQIKDAVRAAEQCGALGVLLNTLFQKTFSAAKAVRSQTAIGANSVS
                     MAAAGVKLALQIFPSIRELNVLFIGAGEMIELVATHFAAQQPAGITVANRTLERGERL
                     AQEFGGRAIVLQDLASQLAQHDVVVTSTASPVPVLGKGAVEKALKARRHKPMFMLDLA
                     VPRDIEAEVGELADVFLYTVDDIAAIVDAGREERRAAAETAEGIIQE"
BASE COUNT         1657 a         3063 c         3237 g         1751 t
ORIGIN      
        1 gaggcgggtt ggcctctggg cggcttgttg aatccgtttc gctgagtgtg gtgagtgtca
       61 ggcgtgggcc tggctgctgg cgcgggtcag cgccacgcca gcggcgataa acgaaccgcc
      121 ggccacccgg ttcatcagct tgagtcggcg cgggctgttc agccaggctt tcagccgggc
      181 gccgcctgcg gcgtaggcgc attgccagct ggtttcaatc acaaaaaacg tcgccgccaa
      241 aatcagcagt tgcgggcctt gcggctgggc cgggtccatg aactgcggaa acagcgcggt
      301 aaaaaacaca atggctttcg ggttgctcat cgccaccaga aagccggtgc gaaacccggc
      361 ccagccgctg ccggcctgtg cttccgcctc accttcggcc aaggcctgcg gctggctgcg
      421 ccaggttttg atccccagcc acagcagata agcagcgcca gcgtacttga ccaggccaaa
      481 cagccattct gatgtcacca gcagcgcgcc cagcccggcg gcggacaagc ccatgatcag
      541 cgccagcgcc agcaacaggc ccagcagggt gggcgtggtg cggcgcaggc caaagcggat
      601 gccgtgggtc atggccagca gcatgttcgg gccgggggtg gcggacacca ccagcacggt
      661 ggcgaagtaa atcagccagg tggacaaggg catgggcagg tctttcgtgg ttcagcaaca
      721 atcagtgcgc cttggcgcct ggtttttccg gcatggccga cgagtgatgc tcggcaatca
      781 gccaggttcc gccgactttg cggtaaacaa aggtgtagcg cgccggcacc tggccgccgg
      841 tggtgtcgaa cacgtacaag cccgagtcaa tcgccacatt cggcgccggg aagcggatat
      901 tgcgctcgac gatcttgccc ttgggttttt tctccaggaa gtggacaaaa taatcgcgga
      961 tttcggcggg gttgtggcgc accttattgg acacggtggg cagcaggatg gcgtccgccg
     1021 cgtagttggc ggcgacgtcg tccgggttgc cggtggccag cgaggcgttc cagcggtcga
     1081 acagggcggc gatttcggct tcgttgccgg cgtgtttgcc ctgcatcgcg ggcatatggc
     1141 cctggccgga atgcacggtc acattcacgc aggccgtgct gagcaaggcg gcagcggcgg
     1201 cagcggcaag ggtgcgggtg aagcgaatca tcggcgtctc ctgttcggtg tttgcgggtg
     1261 gggtgaaacc agcttgcctg atgtccgcac ggctggcgcg gacatcaggc aagctggccg
     1321 ggccaaggcc cgaccaacac gcgcaggctt agctgtcggc gcgttccgcc agaatcgcca
     1381 cggcgggcag ttctttgcct tccaggaatt ccaggaaggc gccgccgccg gtggagatgt
     1441 agctgatttt gtcggtcacg ccaaacttgg cgatggcggc cagggtgtcg ccgccgccgg
     1501 caatcgaaaa cgccgggctg tcggcaatcg ccttggccag ggtttgcgtg ccgctggcaa
     1561 actgatcaaa ctcgaacacg cccaccgggc cgttccacac cacggtgccg gccttggcga
     1621 cgatgtcggc cagttcttgc gccgattgcg ggccgatgtc gaggatcatg tcttgtttgg
     1681 tcacttcgct gaccggcttg agggtgtcgt cggcgttttc cgagaattcc ggggccacca
     1741 ccacatcgct gggcagcggc acggcgccgc cgtgggcctt gatcttggcg atgacttttt
     1801 gcgcttcgcc caccagctcg gcttcggcca ggctcttgcc gatggccttg ccttcggcca
     1861 gcaggaaggt gttggcgatg ccgccgccga cgatcagttg atccaccttg tcagccaggg
     1921 cttccaggat ggtcagcttg gtggacacct tggagccggc gacaatcgcc accagcgggc
     1981 gggccggggc ttccagcgcc ttgcccagcg cgtccagctc ggcggccagc agaatgccgg
     2041 cacaggccag cggggcaaac ttggccacgg cgtgggtgga ggcttcggcg cggtgggcgg
     2101 tgccaaaggc gtcgttgacg aacacatcgc acagcgcggc gtaggcgcgg ccgaggtctt
     2161 cgttgttttt cttttcgccc ttgttcaggc gcacgttttc cagcatcacc acatcgccag
     2221 cgttcaggct gaggccggct tgccagtcgg ccaccaccgg caccggcttg ttcagcagct
     2281 cgcccaggcg ggcggccacc ggagccaggc tgtcttccgg cttgggttcg ccttcggtcg
     2341 ggcggcccag gtgggtcatc aggatcacgg cggcgccgtt gtacagggcg tgctggatcg
     2401 aggccagcga ggcgcggatg cgggtgtcgt cgccgatcac gccgtttttc accggcacgt
     2461 tcatgtccac gcggatcagc acacgcttgc cggccagcgc ctggtcagtg agtttcttga
     2521 atttcatcat gcattccgtg gcaacgccgt tgccgggtca acccggcaac ggcgaagaga
     2581 aaaccgcgcc acccgctcac aggcggcgcg ccgggccgga ttctcaggcg gcgaacatcg
     2641 cgcgggcggt gttcagcatc tggcaggaga agccccattc gttgtcgtac caggccagca
     2701 ccttgaccat attgccgttg gagaccttgg tcagggtgga gtcaaagtgc gaggcttccg
     2761 aggtgtgatt gaagtccatc gacaccagcg gcagcacgtt gtagcccagc acgcccttca
     2821 tcgggccttc ggcggcggct ttgacgatct ggttcacttc ggccacggtg gtggcgcggc
     2881 ctgcttcgaa ggtcaggtca accagcgaca cgttgatggt cggcacgcgc acggcaaagc
     2941 catccagctt gccgttcatt tccggcagca ccaggccgac agccttggcg gcgccggttt
     3001 tggtcgggat catgttctgg gtggcgctgc gggcgcgacg cagatccttg tggcgcacgt
     3061 cggtcagcac ctggtcgttg gtgaaggcgt ggatggtggt catcaggccc ttgacgatgc
     3121 ccaggccgtc gttcagcgcc ttggccaccg gggccaggca gttggtggtg caggaggcat
     3181 tggacaccac ggtcatgtcg gcggtcagca cattgtggtt gacgccgtaa acaatggtgg
     3241 cgtccacatc atcgccgccc ggggcggaga tcagcacctt cttggcgccg gcggtgatgt
     3301 gggcctggca tttttccttg ctggtgaagg cgccggtgca ttccagcacc agatccacct
     3361 tcagctcgcc ccacggcagt tcggccgggt tgcgggtgga gaagaacgga atcacatcgc
     3421 cattgatcac caggcttttc tcgtcgtggc tgacctgggc gttgaagcgg ccgtgcacgg
     3481 tgtcgaattg ggtcaggtgg gcgttggtgg ccaggtcgcc ggaggcgttg accgccacca
     3541 cttcgaattc attgcgcaga ttgttttcgt agatggcgcg cagcacttga cgaccaatgc
     3601 gaccgtaacc gttgatggcg atgcggatgg acatgaaact attctcctgt gaatcgggat
     3661 ggcgcggccc gcgtcctgcg cgggccatca ggggatcagg taaaactcag agcacggact
     3721 tcacggtctg ggcgacattg gccgcggtga agccaaaggc ttcgaacaac tgcttggccg
     3781 gggcggattc gccaaagtgg tccagaccaa ccacgccgcc ttccaggccg acatacttgc
     3841 gccagaagtc ggacacgccg gcttccaccg ccacgcgcgg caggcccgcc ggcagcaccg
     3901 aggcgatata ggccttgtcc tggcggtcga acacattggt ggacggcatg gagaccacgc
     3961 gcacggcaat gccttcttcg gccagcaggg cctgggcttt cagcgccagc tcgacttccg
     4021 agccggtggc gatcagcacg gcgcgggcct tgtccgcctc gcgcagcaca tagccgccgc
     4081 gacggatggc ggcgatttgc tcggcgtcgc ggctgaggaa gggcaggttt tgccggctga
     4141 aaatcagcgt ggacgggcca tcctgacgtt cgatggcggc ctgccaggcc acggcggatt
     4201 ccaccgtgtc agccggacgc caggtgtcca tgttcgggat cagacgcagg gtggcggttt
     4261 gctcgaccgg ctggtgggtc gggccgtctt cgcccagacc gatcgagtcg tgggtaaaga
     4321 caaaaatcgg gttgatcttc atcagcgccg ccatgcgcag ggcattgcgg gcgtattcgc
     4381 tgaacatcag gaaggtggcg ccgtagggct tgaagccgcc gtgcagggtg gcgccgttca
     4441 tgatggcggc catgccgaat tcgcgcacgc cgtaatacac atagttgccg ccttgctcgc
     4501 ggctgacgcc cttggagccg ctccacaggg tcaggttgga gctggccagg tcggccgagc
     4561 cgcccagcag ctcgggcagc gccggggcaa aggcttcgat ggcgttctgc gaggccttgc
     4621 gggtggcgat ggtttcgccc ttggcgttga ttttttcaat cgcggcggcg gcgtgtgcgg
     4681 cccagttggc cggcagttcg ccgcgcatgc ggcgctcgaa ctcggcggct tcggccggga
     4741 aggcggcgcg gtaggcggca aagcgctcag tccattcggc ttcgcgcgcg gcgccggctt
     4801 cgcgggcgtt ccaggcgctg gcgatgtcgg ccggaatctc gaacggcgcg tgcggccagt
     4861 tcaggtgggc gcgagtggcg gcgatttcgg cgtcgcccag cggcgcgccg tgcacatcgt
     4921 ggccgccttc tttattgggc gcgcccttgc cgattttggt gcggcagcag atcagcgtcg
     4981 ggcgggcagt atcggccttg gcggcggcga tggcggcgtc cagcgcggca aagtcgtggc
     5041 cgtcgatggc cggaatcact tgccagccat aggcctcaaa gcgcttgacg gtgtcgtcgg
     5101 tgaaccagcc ttccacatcg ccatcaatcg agatgccgtt gtcgtcgtag aaggcaatca
     5161 gcttgcccag gccccaggtg ccggccagcg agcaggcttc gtgcgagatg ccttccatca
     5221 ggcagccatc acccaggaac acccaggtgt ggtgatcgac aatggcgtgg ccgtcgcggt
     5281 tgaattcagc cgccaggact ttttccgcca gcgccatgcc caccgcgttg gtgatgccct
     5341 gacccagcgg gccggtggtg gtttccacgc ccggcgcgta gcggtattcc ggatggccag
     5401 gagttttgct gtgccactga cggaaggctt tcaggtcgtc aatcgacagg tcgtagccgg
     5461 acaggtgcaa gaggctgtac agcagcatcg agccatggcc gttggacagc acaaagcggt
     5521 cgcggtcaaa ccagccgggg ttggccgggc tgtgcttcag gtggtggcgc cagagggctt
     5581 cggcaatatc agccatgccc atcggcatgc cggggtgacc ggagttggct ttctgcacgg
     5641 catccatgga gaggacacgg atggcgttgg caagatcgct gcgggagacc atgaaatacg
     5701 aacctcgtgt gcttgagtgg cggctggcaa agccggtaac aaaatcatgc gattatcgcc
     5761 gatagcggcc atgggcggca actttgctgc atagaccaat gcgccatggc cgggccaaaa
     5821 acacacatgg cgtccaccca aagggcggac gccatggcaa aaccagccaa aaaaccgggt
     5881 aaggcgggct atttgctgcc ccacagggtc acgccagtgg ccacggtgtt ggcgtccagc
     5941 cgcgactgga aggtatagcc ggcaaacacc ttgcccagcg cgccattggc catcaccccc
     6001 tgcaccacca tattgttgcc atccagatat tgcgagggat tgctaaaggt gccatcgctg
     6061 ccgacattgc cctgcgcctg caagcccacg cgcagattgg cggcggtcag gtcaaagcgg
     6121 gtgccaaacc ggctgcgctc gaaatccacc cacaaccggg cgttttccac attggccagc
     6181 tggctgacgc catcgcgctg cacctgggcc atcgacccct gcaagccaaa ctcggcccca
     6241 ccgtacggca cctgccaggt ggcgctttga tggcgcagca acgcatagtc gccggtcagc
     6301 acgctgacgc ggtaatcctt gctcatcaaa tcggccagat tcaggttggc gccctgctca
     6361 gccacgcctt gccagcgccc ccaggcgatg gccgccgggc ctggcggcgt gggcgctggc
     6421 gccggcatca ggctggaggc cttatccagc tcggccagtt tcagtggcgc cagactggtg
     6481 ttatccgggt tggcgtccga ggtattgtgg cgactgccgg ccttgacctt gccggattca
     6541 tccggctgcg gcggggtggt ggcatctggc gacagcgcct tgccgcgcag cagctccggc
     6601 gccagttggc cacggctgac ctgcaagacg ccatcacgtt gctggttggc aaacagctcg
     6661 cggctgtgcg gcgtttcgca cggtccgccg ccttccggcg cacaaccctg gccaaaaccg
     6721 ctgaccacca cgccgccgga cagtacggca atgcgagtgg tctgcgcatc ggtaaatacg
     6781 gtaaagtcgg tgccccgcac gccaatggcg gccaccgggg tgttgaagcg gaagttctgc
     6841 cgggcgtttt tcaccgcctg accggagatg ctgcgcgcca ccccttcacg caattcaaat
     6901 ttgaagcggc tattggccgg ggtgtgtgaa tccgcctgat aggcgataat gctggcgtcg
     6961 ctattgggcc gcaggatcaa aaaaccgttg tccacggtct tgatatacac atacccgtcg
     7021 gcgcccgtgc tcaggcggtc gccagcctgc acgctgtggc cggccttgac cgcctgaccc
     7081 tgcacgcgca cctcgcccac gctgagcacc accttgccgg cttcatccgc ccaggcagat
     7141 gccgccagac cccacagggc caaggcacac agattgcgga catgggtttg cttgaacatg
     7201 ctgaatctcc ctggacatca cgctgtcggt gtggccgggc ccagaaccgg caacgcgcaa
     7261 tacatataca tttgacagga tagcattttg ggtgtgaccc gacacggaaa acttgcctgc
     7321 cgcatcatgg gcttagcgct attggcgctg gccgcaggca tggtgcgcgc cgatgacggg
     7381 gctggcagcg acaatcccta cctcgacgcc atgcagccgc tgatcgacgg tcgctacgaa
     7441 gaagccggcg aggccttgcg ccgcctggtg gcgcgcgaac ccgaacatgc cggcgcctgg
     7501 ctggacctgg ccattctgca atgtggcctg ggcaatgcca gcgaggccga attgctgttt
     7561 gatgccattg aggcgcgttt tgatccgccg cccggcatcc gcgaaatcat cgccctgcaa
     7621 cgcgccaagg gctgccatgg gccgtcggcg cgctgggaag gccggctcaa gctggggcgt
     7681 ggcagcgaca gcaacgccaa ccagggcgcc agcaacccca atttctctat cggccagggc
     7741 agcagtgtgt tgaatctggt gctgtcgcct gagttcaccc cgcaccgcga ccgctttaac
     7801 caaatcaccc tggacggcgc ggtcagcggc ctgggcaatg gctggcaagg ctatgcccag
     7861 ctgcaaagcc gccgctacga cacccagcgc cgttacgaca gcgaatccgc cctgctgggc
     7921 gccgagcgcc cctggcggct gggtcgttgg gacgcacggc tgggcgccag cgttggcctc
     7981 acccgcctgg gcggcgaaag ctatcaacgc tacgccacca tcagcggctg gctggccccg
     8041 ccgctggcgc tgccaaccgg ttggcagctg ggctggctga acagcttcag ccatgtggtg
     8101 tatccgtcac gctcgcattt tgatgccagc ctgtgggaaa gccgcgccgt gctctccctc
     8161 cagcatgggc cattgtgggt gcaaggcagc gctggctacg gcctggacgt gggcagccac
     8221 gaacgcgctg gcggtgaccg tcatggggtg tttgccagcc tgaacgcccg cgccctgctg
     8281 ggagggggct ggctgggcga gttgagctgg cagtatcaaa actgggccgg tgcacaagcc
     8341 tattcacccg gcctgatcga cgtggcgcgc cgtcagcaca cccagctcac ccgcgccacc
     8401 ctgtggtatg agctgaccaa gcagcaggcg ctgtatctgg aataccgggg cgtgcgcaac
     8461 cgcgagaata tctcgctgtt tgaatatcaa agccgcgcct ggcagttggg ctggcaatgg
     8521 cagtttggcc gctaaacgcg gctcacaaaa ccttcctggc gtggttatgc gagcttgccg
     8581 tacagcgtga caccctgcag tcacacacca ggccagcggt gctttgccaa gtttgctgag
     8641 ccaggccaag ccgtaagacg gacatgatgc tagtcatggt cagcggcgca tcttcgggta
     8701 aaatgccccc ttcctgacag attccgccgc tcatccgcat gcaactggtc gcccttggtc
     8761 ttaaccacca cactgccccg ctcgccatcc gcgaacagct ggctttcccg gccgagcagc
     8821 tggcggacgc gctgcgcgac ctgaccgact cgcgcgccgc gcacgaggcg gccatcctgt
     8881 ccacctgtaa tcgcaccgag atttatgccg gcgccgagaa catcgacgcc gtgctgcact
     8941 ggctggcgcg caatcgcggc ctggacgtgg acaccctgcg gccgtatctg tatctgctgg
     9001 acgccgaagc cgccgcccgc cacgctttcc gggtggcctc cgggctggat tccatggtgc
     9061 tgggcgaaac ccagattgtc ggccagatca aggacgcggt gcgcgccgcc gagcaatgcg
     9121 gcgcgctggg cgtgctgctg aacacactct ttcaaaaaac cttctccgcc gccaaggcgg
     9181 tgcgcagcca gacggcgatt ggcgccaact cggtgtcgat ggccgccgct ggcgtcaagc
     9241 tggccttgca gatcttcccc agcatccgcg agctgaatgt gctgttcatc ggcgcgggcg
     9301 agatgatcga actggtggcc acccactttg ccgcccagca accggccggc atcaccgtgg
     9361 ccaaccgcac gctggagcgc ggcgagcgcc tggcgcaaga gttcggcggc cgggcgattg
     9421 tcttgcagga cttggccagc cagctggcgc agcacgatgt ggtggtgacc tccaccgcct
     9481 cgccagtgcc ggtgctgggc aagggcgcgg tggaaaaagc cctgaaagcg cgccggcaca
     9541 agcccatgtt catgctggac ctggccgtgc cgcgcgacat cgaagccgaa gtgggcgagc
     9601 tggccgatgt gtttttgtac acggtggacg acatcgccgc cattgttgac gccggccgcg
     9661 aagaacgccg cgccgccgcc gagaccgccg aaggcatcat tcaggaaa
//
LOCUS       DAOPYX010000094         9631 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_8327_length_9631_cov_9.124582, whole genome shotgun sequence.
ACCESSION   DAOPYX010000094 DAOPYX010000000
VERSION     DAOPYX010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 9631)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9631)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9631
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8327_length_9631_cov_9.124582"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1142)
                     /gene="groL"
                     /locus_tag="PKN55_06390"
     CDS             complement(<1..1142)
                     /gene="groL"
                     /locus_tag="PKN55_06390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271863.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HNX70904.1"
                     /translation="MAAKEVKFGDSARAKMVTGVNILADAVKVTLGPKGRNVVLDRSY
                     GSPTITKDGVSVAKEIELKDKFENMGAQMVKEVASKTNDIAGDGTTTATVLAQAVVQE
                     GMKYVAAGMNPMDLKRGIDKAVIAVVEELKKISKPCATTKEIAQVGSISANSDTVIGD
                     KIAAAMEKVGKEGVITVEDGSGLEDELDVVEGMQFDRGYLSPYFINQPEKQIAQLENP
                     FVLLFDKKISNIRDLLPVLEQVAKSGRPLLIIAEDVDGEALATLVVNTIRGILKVCAV
                     KAPGFGDRRKAMLEDIAVLTGGTVIAEEVGLTLEKVTLDLLGQAKRVEVGKENTTIID
                     GVGEAAAIQARVEQIRRQIEEATSDYDREKLQERVAKLAGGVAVIKV"
     gene            complement(1220..1507)
                     /gene="groES"
                     /locus_tag="PKN55_06395"
     CDS             complement(1220..1507)
                     /gene="groES"
                     /locus_tag="PKN55_06395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HNX70905.1"
                     /translation="MAIRPLHDRVVIKRLEAEEKTASGIVLPGAAAEKPDMGEVIAVG
                     NGKILENGERRLLEVKKGDKVLFGKYSGQTVKVDGEELLVMREEDIMGIVE"
     gene            complement(1712..2779)
                     /gene="mutY"
                     /locus_tag="PKN55_06400"
     CDS             complement(1712..2779)
                     /gene="mutY"
                     /locus_tag="PKN55_06400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HNX70906.1"
                     /translation="MSVAVSFAEFAPRLIAWQRQHGRHGLPWQVDDPYKVWVSEIMLQ
                     QTQVSTVLGYYPAFLQRFPDVATLAAAPLDDVLAAWSGLGYYSRARNLHRAAGQVMNE
                     FAGRFPDSQDALMTLCGVGRSTAAAIAAFAFARRAAILDGNVKRVLTRCYGIEGWPGD
                     KAIENQLWALAEQLLPDSVAHMAAYTQGLMDLGSLLCVRGQPRCGECPMADGCVARAQ
                     GRQRALPTARPRKAQPLRSTVMLLARNAAGEVWLQRRPDSGVWGGLWSLPELADIGEA
                     PHWLAAAGWTAAPLTPPWPTVQHVFTHFRLDITPQPVQVRATAGTGPADGRWWTQEAA
                     LALGLPAPVRRLLDTRQDSLW"
     gene            complement(2919..4058)
                     /locus_tag="PKN55_06405"
     CDS             complement(2919..4058)
                     /locus_tag="PKN55_06405"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNX70907.1"
                     /translation="MESVLILGNGQAGIALAREIRKLDPRRPITLLGRDAYGFNRSEF
                     VQALGPSQKTLSTEDLLALGEQLRIDMVPNCTAQSIRVADCSVQTDHGEYQADKLVLA
                     LGSSPMLPSFARDLPGLVAAVTHASVVNKLRARLASVKDLIIIGAGQSGCELANTLAD
                     AGKNVTVIEQHAGPLFELMPLDARRYLVEQLEERGVKFHFNARCASASKEGHRAQLAL
                     TNGKHVEGDLVIACIGVVPNTKLASDAGLKVRKGIMVDRKLATSAPDVYALGDCAEIN
                     GVWLPYSQAIHHEVSALARVLTGQNVQLNFPPMPLKLNIPCCPTVIAQPRPGLTGQWE
                     TLMVEHGLNASFISERGQLSGFVLMGEETAEAPFLQDEIPNWMNL"
     gene            complement(4147..5097)
                     /locus_tag="PKN55_06410"
     CDS             complement(4147..5097)
                     /locus_tag="PKN55_06410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009115845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HNX70908.1"
                     /translation="MTLTELRYIVAVARERHFGRAAAACFVSQPTLSIAVRKLEEELG
                     ITLFERGSNEITVTDTGERIIEQAQRVLEEVSVIKTLASELKNELVGPFRLGVIFTIG
                     PYLLPALIPALRELAPDMPLYLEENYTARLADMLKRGEVDAVIVAEPFDEPGVVTYPL
                     YDEPFVVATPRTHPWAKRKTISSAELANESVLLLTQGNCFRDQVLALCDRLQQPAAPG
                     GHALSRTLQGSSLTTIRHMVASGVGITVMPVTSVGPGDEALLGIVPFAAPAPTRRVVL
                     ACRKQFYRRNAIAALCQAVRHSALPGVQWLDNAPMSAEAS"
     gene            complement(5094..5360)
                     /gene="minE"
                     /locus_tag="PKN55_06415"
     CDS             complement(5094..5360)
                     /gene="minE"
                     /locus_tag="PKN55_06415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017510129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HNX70909.1"
                     /translation="MSLIDRWLGRSMEKQSAAVAHERLQIILTHERNGRTGTPDYLPA
                     LQKELLAVISKYVAVSAENIKVQLERQDQLEVLEVNIVLPDSRG"
     gene            complement(5363..>5626)
                     /locus_tag="PKN55_06420"
     CDS             complement(5363..>5626)
                     /locus_tag="PKN55_06420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215463.1"
                     /note="ATPase; with MinC inhibits cell division by
                     blocking formation of the polar Z ring septums; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HNX70910.1"
                     /translation="PARVDKGEMLSVENICDILCIPLIGVVPESQAVLLASNEGVPVI
                     HKADTDVAQAYQDVVSRFMGDELPMRFLDAPPKPGLLKRLFGG"
     assembly_gap    5627..5726
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5727..5851)
                     /locus_tag="PKN55_06425"
     CDS             complement(<5727..5851)
                     /locus_tag="PKN55_06425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNX70911.1"
                     /translation="MAKIIVVTSGKGGVGKTTTSANFATGLALQGHRTVVIDFDVG"
     gene            complement(6163..6951)
                     /gene="minC"
                     /locus_tag="PKN55_06430"
     CDS             complement(6163..6951)
                     /gene="minC"
                     /locus_tag="PKN55_06430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014088191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004857 - enzyme inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HNX70912.1"
                     /translation="MTATHSLSVLDIKSASLDVLSLLLRSADPELLRNELSARLARRP
                     KAFASEPFLLDVSALDDSVADALPQLCELLQSQGVRIIGVRHSSAAVGEQARALGWGW
                     FPAKLERPSAPVAEPAEPVSVPESLPEPVAAEPEPAPQVSGGGLGCTGKTLVVDRPVR
                     AGQQVYARDGNLVVLAAVSPGAELIADGDIHVYARMRGRALAGARGNVDARIFMQQMA
                     AELVSIAGVYRTLEEDLPETLHERAVQVSLDGSKLVLAAMAVSG"
     gene            7524..8087
                     /locus_tag="PKN55_06435"
     CDS             7524..8087
                     /locus_tag="PKN55_06435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012697995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HNX70913.1"
                     /translation="MFNNNEQLKTLSQAQFDKAVRLSNIVLASAERFANLHLELSKKL
                     LADQAATTKALVELKDPKAVLEYQASLAQPGIDQAFSVARSVYDAALQTQTDIQSFVE
                     EQVLEFNKQLCANLDKVSKNAPAGSEVAINAVKNMLTSATAAYDNVTRTAKKVGTELA
                     EAGVEAAANSAKAASAAVARTTAAAKK"
     gene            complement(8257..9258)
                     /gene="hemB"
                     /locus_tag="PKN55_06440"
     CDS             complement(8257..9258)
                     /gene="hemB"
                     /locus_tag="PKN55_06440"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014086137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HNX70914.1"
                     /translation="MIPVSRSFPATRMRRMRRDDFSRRLMREHTLTADDLIYPVFVLE
                     GERREEPVVSMPGVVRQSLDRLLATAEEAVKLGIPALALFPVIESGKTLDGMEACNPE
                     GLVPTVVRTLKREFPQLGVITDGALDPYTSHGQDGVIDEHGYVLNDITTEILVKQALC
                     HAEAGVDMVAPSDMMDGRIGALRQALEAHGHIHTRIFSYAAKYASAFYGPFRDAVGSA
                     GNLGKADKKTYQMDPANSDEALHEVAMDLAEGADLVMVKPGLPYLDVIRRVKDQFGVP
                     TFAYQVSGEYAMLKAAAQNGWLDERACMLESLLAFKRAGADGVLTYFALDAARALRG"
BASE COUNT         1957 a         3140 c         2756 g         1678 t
ORIGIN      
        1 accttgatca cggccacacc gccggccagc ttggccacgc gctcttgcag cttctcgcgg
       61 tcgtagtcgc tggtggcttc ttcgatctgg cgacggattt gctcaacgcg cgcctggatg
      121 gcggcagctt cgcccacgcc gtcgatgatg gtggtgtttt ccttgcccac ttcgacgcgc
      181 ttggcttggc ccagcagatc cagggtgacc ttttccaggg tcaggcccac ttcttcggcg
      241 atcacggtgc cgccggtcag cacggcgatg tcttccagca tggccttgcg gcgatcgcca
      301 aagcccggag ccttgacggc acacaccttc aggatgccgc ggatggtgtt caccaccagg
      361 gtggccagcg cttcgccgtc cacgtcttca gcgatgatca gcagcgggcg gccggacttg
      421 gccacttgct ccagcaccgg cagcaggtcg cggatgttgc tgattttctt gtcgaacagc
      481 agcacaaacg ggttttccag ctgggcgatc tgcttttccg gctggttgat gaagtacggg
      541 gacaggtagc cgcggtcaaa ctgcatgcct tccaccacgt ccagctcgtc ttccaggccc
      601 gaaccgtctt ccacggtgat cacgccttct ttgccgactt tttccatggc ggcggcgatc
      661 ttgtcgccaa tcacggtgtc gctgttggcg gagatggagc cgacctgggc gatttccttg
      721 gtggtggcgc acggcttgga gattttcttc agctcttcca ccacggcgat cacggccttg
      781 tcgatgccgc gcttcaggtc catcgggttc atgccggcgg ccacgtactt catgccttct
      841 tgcaccacgg cttgcgccag cacggtggcg gtggtggtgc cgtcaccggc gatgtcgttg
      901 gtcttggaag ccacttcctt gaccatttgt gcgcccatgt tctcgaactt gtctttcagt
      961 tcgatttcct tggccaccga cacgccatcc ttggtgatgg tcggcgaacc atagctgcgg
     1021 tccagcacca cgttgcggcc cttcgggccc agggtcactt tgacggcgtc ggccaggata
     1081 ttgacgccgg tcaccatctt ggcgcgagcg gaatcaccga acttgacttc tttggcagcc
     1141 atttttttac tccgaaattc tgtcgttgtc gtacttgacg attactaatc tgaatacagc
     1201 agggtcaaca ggcggttgat tattcaacga tgcccatgat gtcttcttcg cgcatcacca
     1261 gcagctcttc gccgtccacc ttgacggtct ggccggagta tttgccgaac agcaccttgt
     1321 cgcccttttt cacttccagc aggcggcgtt cgccgttttc caggatcttg ccgttgccca
     1381 ccgcgatcac ttcgcccatg tccggcttct cggcggctgc gcccggcaga acgatgccgg
     1441 aagcggtttt ttcttcagct tcgagacgct tgataactac acggtcgtgc aggggacgaa
     1501 tagccatgag aacttctcct aaattcgggt tggatgtcga gaaacaaaaa agcaagtcaa
     1561 atcatcgaga tggcctagct gattagcact caacgcttgc gagtgctaat agtatggacg
     1621 cacgccaagg ttttcaagag cggaaaatca ttttttttgt ccgccaccgg ccacatgcgc
     1681 agccagccag gcttgccccg gccagcgccg ctcaccacaa actgtcctgc cgggtgtcca
     1741 gcaagcggcg caccggcgcc ggcaagccca gcgccagcgc ggcctcctgc gtccaccagc
     1801 gcccatcggc cgggccggtc cccgccgtgg cgcgcacttg cactggctgc ggggtgatgt
     1861 ccagacgaaa atgggtgaac acatgctgca cggtgggcca cggcggcgtc agcggcgccg
     1921 ctgtccagcc cgccgccgcc agccaatggg gcgcttcgcc gatgtccgcc agctcgggca
     1981 ggctccacag cccgccccac acgccgctgt ccgggcggcg ttgcagccac acctcgccag
     2041 cggcattgcg cgccagcagc atcaccgtgc tgcgcaaggg ctgcgccttg cgcgggcggg
     2101 cggtgggcag cgcacgctgg cggccctgcg cgcgcgccac gcagccgtcg gccatcgggc
     2161 attcgccgca acgcggctgg ccgcgcacgc acagcaagct gcccaaatcc atcagcccct
     2221 gggtgtaggc ggccatgtgc gccaccgagt ccggcaagag ctgctcggcc agcgcccata
     2281 actggttttc gatggcttta tcgccgggcc agccctcaat gccatagcag cgggtgagca
     2341 cgcgcttgac attgccatcc agaatcgccg cacggcgggc aaaggcaaag gcggcaatcg
     2401 ccgccgcggt ggagcgcccc accccgcaca gcgtcatcaa ggcatcctgg ctgtcgggaa
     2461 aacggccggc aaattcgttc atcacctggc cggcagcgcg gtgcagattg cgcgcgcggc
     2521 tgtaatagcc cagcccgctc caggccgcca gcacatcgtc cagcggggca gcagccaggg
     2581 tggcgacatc gggaaagcgc tgcaagaagg ccgggtaata ccccagcacg gtggacacct
     2641 gggtttgctg caacatgatt tccgacaccc acaccttgta ggggtcgtcc acctgccagg
     2701 gcaggccgtg gcggccatgc tggcgctgcc aggcaatcag gcggggggca aattcggcaa
     2761 aggacacagc aacagacatg gcgcaggcaa ccagcaagac aagaggcggc agcatagccg
     2821 cgccagcgca aaaaacaaag ctgccaaaaa cacgcgcgcc atcgccggat caaccgggga
     2881 tggcgcgcca tgcaggatgt gcggtcagcg gatggggatt acagattcat ccagttgggg
     2941 atttcgtctt gcaggaacgg cgcttcggca gtttcttcgc ccatcagcac aaagccggac
     3001 agctggccgc gctcgctgat aaagctggca ttcaggccgt gttccaccat cagggtttcc
     3061 cactggccgg tcaaacccgg gcgcggctgg gcaatcaccg tagggcagca tgggatgttg
     3121 agcttcagcg gcatgggcgg gaagttcaac tggacattct gtcccgtcag cacacgcgcc
     3181 agtgccgaga cttcgtggtg aatggcctgg ctatacggca gccagacgcc gttgatttcg
     3241 gcgcaatcac ccagcgcgta gacatccggc gcactggtgg ccagcttgcg gtccaccatg
     3301 atgcctttgc gcactttcag gccggcatcg ctggccagct tggtgttggg caccacgcca
     3361 atgcaggcaa tgaccagatc accctcgaca tgcttgccat tggtcagcgc cagctgggca
     3421 cggtggcctt ctttgctggc actggcgcaa cgggcattga agtggaactt caccccgcgc
     3481 tcttccagtt gctcaaccag atagcggcgc gcatccagtg gcatcagctc gaacagcggg
     3541 ccggcgtgtt gttcgatcac cgtgacattc ttgccggcat ccgccagggt attggccagc
     3601 tcgcagccag actggccggc gccaatgatg atcaagtcct tgaccgaggc caggcgggcg
     3661 cgcagcttgt tcaccacgct ggcatgggtg acagccgcca ccaggccagg caaatcgcgg
     3721 gcgaagctgg gcaacatcgg gctggaaccc agcgccagca ccagcttgtc agcctgatat
     3781 tcgccatggt cggtctgtac actgcaatcc gccacacgga tcgactgcgc cgtgcagttg
     3841 ggcaccatat caatgcgcaa ctgttcaccc agcgccagca aatcctcggt gctcagggtt
     3901 ttttgcgagg gccccagcgc ctgcacaaac tcggaacgat tgaacccata cgcatcgcgc
     3961 cccagcaggg tgatcgggcg acgcgggtcg agtttgcgga tttcccgggc cagggcgatg
     4021 ccggcctgac cattaccaag aatgagcacg gattccattt gcagtcacta ttccagtgcg
     4081 acggtgaaaa cactccatca taaaggatga tggggatggt cggcaaagcc gacagcatgc
     4141 aagcgctcag gatgcctcgg cggacatcgg cgcattgtcc agccattgca cgccgggcaa
     4201 ggcactgtga cgcaccgcct gacacagcgc agcgatggcg ttgcgccggt aaaactgctt
     4261 gcgacaggcc agcaccaccc gccgggtggg ggcgggggca gcaaacggca caatgcccag
     4321 taaagcctca tcccccgggc ccaccgaggt cactggcatc acggtgatgc ccacgccact
     4381 ggccaccata tggcggatgg tggtcaagga gctgccctgc agggttcgtg acagtgcatg
     4441 accacccggc gctgccggct gctgcaagcg gtcgcacagc gccagcacct gatcacgaaa
     4501 acagttgccc tgagtcagca gcagcacgct ttcattggcc agctcggccg agctgatggt
     4561 tttgcgcttg gcccagggat gggttctggg cgtagccacc acaaagggct catcgtacag
     4621 gggataggtg accacgccag gctcgtcaaa gggttcggcg acaatcaccg catccacctc
     4681 gccgcgcttg agcatgtcgg ccagccgggc ggtgtagttt tcttccaggt agagcggcat
     4741 atccggcgcc agctcgcgca gggccggaat cagcgccggc agcagatacg gcccgatggt
     4801 gaaaatcacc cccaggcgga acggcccgac cagctcgttt ttcagctcgc tggccagggt
     4861 tttgatcacc gagacttctt ccagcacccg ctgcgcctgc tcgatgatgc gctcgccggt
     4921 gtcggtaacg gtgatttcat tgctgccgcg ctcgaacagg gtgatgccca gttcttcttc
     4981 cagcttgcgc acggcaatgc tcagcgtggg ctgactgaca aagcaggccg ccgccgcccg
     5041 gccaaagtgg cgctcgcgcg ccacggccac gatgtagcgc aattcggtga gagtcatccg
     5101 cgcgaatccg gcaagacgat attcacttcc aggacttcca gctgatcctg acgctccagc
     5161 tgcaccttga tgttttctgc cgacacggcg acgtatttgg aaatcaccgc caacagctct
     5221 ttttgcagcg ccggcagata atcgggcgtg ccggtgcggc cattgcgctc atgggtgagg
     5281 ataatctgca aacgctcatg cgccacggct gccgactgtt tttccatgct gcgtcccagc
     5341 cagcgatcaa tcaacgacat ggtcagcctc caaaaaggcg cttgagcagg ccaggcttgg
     5401 gcggtgcatc cagaaagcgc atcggcagtt cgtcgcccat gaaacggctc accacatcct
     5461 gatatgcctg cgccacatca gtatcggcct tgtggatcac cggcacgcct tcattggagg
     5521 ccagcagcac cgcctgggat tcaggcacca cgccaatcag cggaatgcac aggatgtcgc
     5581 aaatgttttc aactgacagc atttcgccct tgtccacccg cgccggnnnn nnnnnnnnnn
     5641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5701 nnnnnnnnnn nnnnnnnnnn nnnnnnccga cgtcaaaatc gatcaccacg gtgcgatggc
     5761 cctgcaaggc caggccagta gcgaaattgg cgctggtagt ggttttaccg acgcccccct
     5821 tacccgaggt cactacaata atttttgcca cggtattttc ctgaacgttg aacgtttctg
     5881 ctggctggcg cgcaagcacg cggcaagccc cttcacggag ccctgtgtgc tcgcgcggca
     5941 cgcgcagttt acacacagta tttcaggatt tcgagcgcgg ctcaaagcca tattgcacaa
     6001 ggctttgctc aagagttcac gattaagtag atttacttag aaaataagca agacgcatga
     6061 gaaagcccgg ttttccggcc acatcctcaa ggagaggcgc ccgcagacac cacatcgtga
     6121 cccccggtca gggccacggt agagaaatgg tttccgctac actcagcccg acaccgccat
     6181 ggccgccagc accaacttgc tgccatccag cgacacctgc acggcacgct catgcagcgt
     6241 ctccggcagg tcttcttcca aagtacgata caccccggca atcgacacca gctcggcagc
     6301 catctgctgc atgaaaatcc gcgcatcgac attgccgcgc gcaccggcca atgcccggcc
     6361 acgcatgcgg gcatacacat ggatatcgcc atcggcaatc aactcggcgc cagggctgac
     6421 agcagccagc accaccagat tgccatcccg ggcatacacc tgctggccgg cgcgcaccgg
     6481 acgatccacc accagggttt tgcccgtaca gcctagccca ccacccgaca cttgcggcgc
     6541 gggctcgggc tcagccgcca cgggctcagg caaggattcg ggtacgctga cgggttcggc
     6601 gggttcagca acaggcgcgg atgggcgttc cagcttggcc ggaaaccacc cccagcccag
     6661 cgcccgcgcc tgctcgccca cggcggcgct gctgtggcgc acgccgatga tgcgcacccc
     6721 ctgggattgc aacagttcac acagttgcgg cagagcatcg gccacgctgt catccagcgc
     6781 cgacacatcc agcagaaaag gttcgctggc aaacgccttc ggtcggcgcg ccagccgtgc
     6841 cgacaattcg ttgcggagca gttcagggtc ggctgaacgc aacagcaggg acagcacatc
     6901 cagcgaggcg gatttgatat caaggacaga cagggaatgg gtggcggtca tggtgtgaac
     6961 ggtcgctccc ggaaacagga caccgatggc atgggcacat ggcgccgggc cgggcgggag
     7021 cactggcaag caagcatggg cacaccttct gtcggtcgtc aggtgaagac cagtcagccc
     7081 acgccagcgc aggacccagt gcgatgacga atggctcaac cggtcaggaa atatggaaga
     7141 tgagtgtacc tgctggccgg gtcagcttca aggcgggcaa accctgtaca ccctggccag
     7201 actggcgtat tccgcccgct tgccagtcgc acagaggggt ggctgctgtg gcgcgcaaaa
     7261 ccatggtcat gtgacaaaaa cttgtgacca actgttgcaa ctgaataaaa atccaataaa
     7321 aaatagccac ttgcccaatg ttgcagcgca acaaaacact tgacgacagt ttcagaatgc
     7381 agtaatcttc aactcaggat gctgcaacgc aacatcacga tcgattccag gcggacacgc
     7441 agcagacggc ggtacacttc gtcctggcac atccaccgga agacgaagac aacaccaacc
     7501 ttgaccacgc gaaaggaaac accatgttca acaacaacga gcaactgaaa accctgagcc
     7561 aagcccaatt cgacaaggcc gtgcgcctgt ccaacatcgt gctggccagc gccgaacgct
     7621 ttgccaacct gcatctggaa ctgtccaaga agctgctggc tgaccaagcc gccaccacca
     7681 aagccctggt ggaactgaaa gatccgaagg ccgtgctgga atatcaagcc agcctggcac
     7741 aaccgggcat cgaccaagcc ttctccgtgg ctcgctcggt gtacgacgct gccctgcaaa
     7801 cccagaccga catccagtcc ttcgtggaag agcaagtgct ggaattcaac aagcagctgt
     7861 gcgccaacct ggacaaagtc tccaagaacg cgccggccgg ttccgaagtg gccatcaacg
     7921 ccgtcaagaa catgctgacc tcggccaccg ccgcttacga caacgtgacc cgcaccgcca
     7981 agaaagtcgg caccgaactg gctgaagccg gcgtggaagc cgctgccaac tcggccaagg
     8041 ccgccagcgc cgccgtggct cgcaccaccg ctgctgccaa gaagtaattc aagcaggacc
     8101 cgctgagtca aggttgcggc gccgtttcgc atcggcgccg cgcaccatga ccgcacacag
     8161 aatttcgccc catggccccc gccgatgggg ctttgttttt tggcgctggc caccaagtgg
     8221 cggcgcgcca acacgccgcc gctcaccgaa gaagcattac ccgcgcaagg cgcgcgcagc
     8281 gtccagggca aaatacgtca gcacgccatc cgccccggcg cgcttgaacg ccagcaggct
     8341 ttccagcatg caggcgcgct cgtccagcca gccattttgc gcggcggcct tgagcatggc
     8401 gtactcgccc gacacctgat aggcaaacgt cggcacgcca aactgatcct tcacccggcg
     8461 aatcacgtcc agatacggca gccccggctt gaccatcacc aaatccgcgc cctcggccag
     8521 atccatcgcc acctcgtgca gcgcttcatc actattggcc ggatccatct gataggtttt
     8581 cttgtccgcc ttgcccagat tgcctgccga acccaccgca tcgcggaacg ggccgtagaa
     8641 cgccgaagcg tatttggcgg cgtagctgaa aatgcgggtg tgaatatggc catgcgcctc
     8701 cagcgcctga cgcaaagcgc caatccgccc gtccatcatg tccgacggcg ccaccatatc
     8761 caccccggcc tcggcatgac acaaagcctg cttgaccaga atctcggtgg tgatgtcgtt
     8821 cagcacataa ccgtgctcgt caatcacacc atcctggcca tggctggtgt acggatccag
     8881 cgcgccatcg gtaatcaccc ccaactgcgg aaactcacgc ttgagcgtgc gcaccacggt
     8941 aggcaccaag ccctctggat tgcacgcctc catgccatcc agcgtcttgc ccgactcgat
     9001 caccggaaac aaagccagcg ccggaatccc cagcttgacc gcctcttccg ccgtggccag
     9061 caaacgatcc agactctgcc gcaccacccc cggcatcgac accaccggct cctcacgccg
     9121 ctcgccctcc agcacgaaca ccgggtaaat caaatcatcc gccgtcagcg tgtgctcacg
     9181 catcaaacga cgggaaaaat catcacgccg catgcgacgc atgcgggtag cgggaaaact
     9241 gcgggaaaca gggatcatga cgcgtccaga caaacaatca acaccacgcc caccccaagg
     9301 gcagacgcac gaaaatacag tcactcaagc agcgtgaaac cgctgccaag gccgcagtgc
     9361 tcaggcgcgg gagagactcg cctggcggca ataggttcga caagattaat gaatgggagg
     9421 cgacgtcgcc catcagcctc agcgcgctgc acacgtaaac atgaggaaag gaaacacgca
     9481 acacgccgcc ccaggtctgt tcacggcacg ggccgcagcg ccccaccggg tttttgggcc
     9541 ccataaagcc cgctgagcat cgcagaaaac cggggggctt tccgccacgg ctaagcggcg
     9601 gcgcagccga cgcgccattc cgtggcggcc c
//
LOCUS       DAOPYX010000095         9558 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_8405_length_9558_cov_12.282437, whole genome shotgun sequence.
ACCESSION   DAOPYX010000095 DAOPYX010000000
VERSION     DAOPYX010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 9558)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9558)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9558
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8405_length_9558_cov_12.282437"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..512)
                     /locus_tag="PKN55_06445"
     CDS             complement(<1..512)
                     /locus_tag="PKN55_06445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012698462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HNX70915.1"
                     /translation="MMRRGLGVAVGALVLMSSLSACVPVVVAGAGAAAMVGTDRRTTG
                     SYTDDQTFEVKLAGQIADKVPGVHVNVTSFNRAVLLTGEVPDDNARAQVDFLARAQPG
                     VRKLYNYVVIAPNSTLGNRLNDSGLSAKVRTRLLQGKNFSSNHIKVVTERGEVYLLGL
                     VSEAEGQEAA"
     gene            complement(509..1099)
                     /locus_tag="PKN55_06450"
     CDS             complement(509..1099)
                     /locus_tag="PKN55_06450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008952146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008968 - D-sedoheptulose 7-phosphate
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoheptose isomerase"
                     /protein_id="HNX70916.1"
                     /translation="MDLIQRVNEHFIESIAAKQQAMEVLSPGIAVAAEKMVACLMNEG
                     KILACGNGGSAADAQHFAAEMVGRFEKERPGLAALSLATDTSALTAIGNDYDFDMVFS
                     KQVRALGQTGDILLAISTSGNSANVIEAIHSAHERQMTVIAFTGKDGGKIGELLTSED
                     ILLNVPAFRTARIQEVHILMIHALCDAIDYMLLGGE"
     gene            complement(1117..1449)
                     /locus_tag="PKN55_06455"
     CDS             complement(1117..1449)
                     /locus_tag="PKN55_06455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008952145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HNX70917.1"
                     /translation="MNERGDAAEARALAWLQARGLRCLARNYRCRLGEIDLIMQDGDT
                     VVFVEVRQRRSARFGGAAASITPAKQARLTLAAEHYLQTLSSVPPCRFDAVLFDGAQP
                     PHWVQNII"
     gene            complement(1442..2473)
                     /locus_tag="PKN55_06460"
     CDS             complement(1442..2473)
                     /locus_tag="PKN55_06460"
                     /inference="COORDINATES: protein motif:HMM:NF016251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator"
                     /protein_id="HNX70918.1"
                     /translation="MAAAPAAGPNYSTDLNDVAALHSPLTAILPAPVAQAVAQSGQLS
                     APAGPAPRVAVLLPASGPLADAGQVLRQGIEAAWKVDADAELAWYDTEADNVRAQYQR
                     ALDDGASVVIGPLAREAVAELASIASVPTLALNTVSGSAAPTLYAFHLAVESEGAQMA
                     LAMHRAGHLFPLLVVGADPLSQRLAQGWRAAWTDIHGRSPRWVVQWPAERARLNELAA
                     QADAVVLAVPGRDWPALRPLLSALPAYAAASLSDFARPQLAWPGVVQLDMPWLLSPQA
                     APAARYPRPAEPLTLATERLYAQGIDAFRLARRLAGRDTPGLTLDGVTGWLSLRGREV
                     QRSLRAVDE"
     gene            2525..3418
                     /gene="rsmI"
                     /locus_tag="PKN55_06465"
     CDS             2525..3418
                     /gene="rsmI"
                     /locus_tag="PKN55_06465"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012698466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HNX70919.1"
                     /translation="MHTPLFPIVEDAQASLVNGTLYVVATPIGNVADISLRALAVLAR
                     ADLICAEDTRVTGQLLTRYGIQSKLVSVREHNERAMADKVCNWLAEGQLVAQVSDAGT
                     PAVSDPGARLVAAVRAAGHPVCAIPGACAAVTALAASGVTTAEWLFHGFLPPKRGARC
                     KQLESWRDAHYAVVLYEAPHRVAECAADIAVTLGESRLVTLARELTKTFETVRTLPAG
                     ELAQWIASDSNQQRGECVLIIDAAAPSAAETAIDAQAEHTLRVLLAELPPKQAAKLAA
                     TLTGAPRDALYQLAVSLRHSE"
     gene            3485..3569
                     /locus_tag="PKN55_06470"
     tRNA            3485..3569
                     /locus_tag="PKN55_06470"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3519..3521,aa:Leu,seq:caa)
     gene            complement(3723..5666)
                     /locus_tag="PKN55_06475"
     CDS             complement(3723..5666)
                     /locus_tag="PKN55_06475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009118603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoX family phosphatase"
                     /protein_id="HNX70920.1"
                     /translation="MSRLIKTDDSVVNASSNPAFSAILEASLDRRGLLKGLGASLALG
                     LAGGTVSQLAQAANAAPTAPLSLGFTGLPFSTDDVVHVPAGYSTQVLYAWGDPVGVPG
                     KQPAFKQDASNSAEEQALQAGMHHDGMAYFPLPFGSAHSKRGLLAINHEYTDDGLLHP
                     DGMAHWNDKKTAKAQAAVGVSVIEVEDTGKGWRVVRPSALARRITATTPCAISGPAKG
                     HALMKTQDDPKGVRVLGTLNNCANGQTPWGTYLTCEENWSDYFANNGELSADDKRYGI
                     KATDAGYKWSETDFRFDRQITPNEAHRFGWVVEIDPFDAKSTPVKRTALGRVKHEGAM
                     LVLAPDNRVVVYMGDDQRFEYIYKFVSSKPYNPKNRKANFDLLDHGTLYVARFDQDNT
                     GRWLELTHGKNGLTAANGFASQADICIHTRRAADLVGATKMDRPEWIAAHPHKPYEIY
                     CTLTNNSERGKDGKPGVDAANPRVDNSFGQIIRWFEGDNNPATTEFVWDLFALGGSPT
                     GKGDKKASVQGDTFGSPDGLKFASNGLLWVQTDVSTSTLNEKDYAGMGNNQMLAVIPE
                     RGEFRRFLTGPRGCEITGIAFTPDNRTMFINIQHPGETSSERSDPAAPKAVSAWPDGA
                     AGGRPRSATVVIRRDDGGVIGVA"
     gene            complement(5877..6959)
                     /locus_tag="PKN55_06480"
     CDS             complement(5877..6959)
                     /locus_tag="PKN55_06480"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HNX70921.1"
                     /translation="MMKWFLAALVAANVVVALLGALSKPAPTDIKSRESNANQIKLAG
                     QGNEPSPLEALTAPKPGSALAAAQQEAAKNPPPAAAPAAPATPPVTTLAAALAPEPAK
                     PTPEPAKAPEHKPAAPKPEPLKEASKPMPAPVSKPEPKPAEVKKPEAKPEPKAEAKPA
                     EVKKPEPKVELKDSKPAEKKPEPAKPEVAKTDAKVADKKADAKPDAKPKLVCYQATDL
                     DSAAASKLKAALNKAGLAAKMHETVQETGRTGGKFWVYLPSYANRDEAVKQSAALKDK
                     GFDNYIVNTEGENKNGLSMGLFSQEDSAKAMVKRLNDSGYPAKLTPRGGTAVTNASVR
                     LDGLDEAGSAALSKAGVSMKPVSCKP"
     gene            complement(6956..>7459)
                     /locus_tag="PKN55_06485"
     CDS             complement(6956..>7459)
                     /locus_tag="PKN55_06485"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HNX70922.1"
                     /translation="VAMAWQWPQAQCAGVTCQYDTQRLGADRWMALLGAWGRAPGAKV
                     VAAAGTALTVDALSADGVFLGGTIAPGLQLMRDSLAQGTARLGRPDGEYQDFPHTTAD
                     AIYSGCLNAMLAPIMLCVQRMQALGPAPTVYLTGGDANLVARHLPLDCQIVDNLALDG
                     LARITRP"
     assembly_gap    7460..7559
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7560..8029)
                     /locus_tag="PKN55_06490"
     CDS             complement(<7560..8029)
                     /locus_tag="PKN55_06490"
                     /inference="COORDINATES: protein motif:HMM:NF019884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HNX70923.1"
                     /translation="MKAPVWAVLRRLSDGGVHSGERLAEELGCSRASVNARIQALADG
                     YQLRIDRVRGQGYRLLSPIDWLDAAAIQAGAPAGVRVEVVEETASTNSDVLARAAAGE
                     ASGLARLAESQTAGRGRFGRRWLAPLGGGLTFSLLWRFECGVGQLSGLSLVVGVA"
     gene            complement(8033..8524)
                     /gene="rfaE2"
                     /locus_tag="PKN55_06495"
     CDS             complement(8033..8524)
                     /gene="rfaE2"
                     /locus_tag="PKN55_06495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018747041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase"
                     /protein_id="HNX70924.1"
                     /translation="MNYPAPEFERKLCPPEQLAERLSALARPLVMTNGCFDILHRGHV
                     TYLAQARALGASLVVALNTDASVRRQGKGEDRPINPLAQRAAVMAALESVSLVTWFDE
                     DTPAKLIDVVRPDVLVKGGDWPIERIVGSAETLARGGQVHSIPFEFETSTTAMLNRIR
                     AQG"
     gene            complement(8534..9367)
                     /locus_tag="PKN55_06500"
     CDS             complement(8534..9367)
                     /locus_tag="PKN55_06500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018150655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HNX70925.1"
                     /translation="MLYPELFAQLEKARWDMATDIPWDQFNPELLTDEQAQTIRLNAI
                     TEWSALPATEMFLRDNRHDSDFSAFMSIWFYEEQKHSLVLIEYLRRFRPELVPTEEEL
                     HAVRFEFDPAPPLETLMLHFCGEVRLTQWYRCASEWHTEPVIQHIYSTLSRDEARHGG
                     AYLKYMKQAIDRFGDQARLAFAKIGMLMAASGRSGKPLHPTNLHVNQALFPNDTVQSR
                     LPDPEWLEDWLTHKIRFTAEWESRVVGGILRNLSSLFERPFESVQQLNRYRKELVAAT
                     A"
BASE COUNT         1670 a         3105 c         2965 g         1718 t
ORIGIN      
        1 tgcgccgcct cctgcccttc tgcctcggac accagcccca gcaaatagac ttcgccgcgc
       61 tcggtcacca ccttgatgtg gttggacgag aaattcttgc cttgcagcag acgggtgcgc
      121 accttggcgc tcagtccgct gtcgttcagc cggttgccca gggtggagtt gggggcaatc
      181 actacatagt tatacagctt gcgcacacca ggctgcgccc gcgccagaaa atccacctgc
      241 gcacgggcat tgtcgtccgg tacttcgccg gtcagcagta cggcgcggtt gaacgatgtg
      301 acattgacat gcacaccggg caccttgtcg gcaatttgcc cggccagttt gacttcaaac
      361 gtctgatcgt cagtataaga cccggtggtg cgccgatccg tgcccaccat ggcagcagca
      421 cccgctccgg ccacgaccac cggcacgcag gccgacaagg aagacatcag caccagcgcg
      481 cccacggcca cacccaatcc gcgtcgcatc attcaccccc cagcagcatg taatcaatgg
      541 catcacacag cgcatgaatc atcaggatat gcacttcctg gatgcgtgcg gtgcggaagg
      601 ccggcacatt gagcagaatg tcctcactgg tcagcaactc gccaatcttg ccgccatcct
      661 tgccggtaaa ggcaatcacg gtcatctgcc gctcgtgggc ggagtgaatc gcctcaatca
      721 cattggcgga attgcccgag gtggaaatcg ccaagaggat gtcgccggtc tggcccagcg
      781 cgcgcacctg cttggagaac accatatcaa agtcgtagtc gttgccaata gccgtcaagg
      841 ccgaagtgtc agtggccagc gacagcgctg ccaggccagg gcgctctttt tcgaaacgcc
      901 cgaccatttc ggcggcaaag tgctgggcgt ccgccgccga gccgccattg ccgcaggcca
      961 gaatcttgcc ttcattcatc aggcaagcga ccattttttc ggcggccacg gcgatgccgg
     1021 gcgacagcac ttccatggct tgctgcttgg cggcaatgct ttcgataaag tgctcgttga
     1081 cgcgctggat caaatccatc gggcttcctt cttccttcag ataatgttct gcacccagtg
     1141 tggcggctgc gcgccgtcaa acagcacggc gtcaaagcgg cacggcggca cgctggacag
     1201 ggtttgcagg tagtgttcgg cagccagcgt caaacgcgcc tgcttggccg gggtgatgct
     1261 ggcggcagcg ccgccaaagc gggcgctgcg gcgctgccgc acctcgacaa acaccacggt
     1321 gtcgccatcc tgcatgatca aatcaatttc gcccagccgg caacggtagt tgcgcgccag
     1381 gcaacgcaag ccgcgcgctt gcagccaggc cagcgcgcgc gcctcggcgg cgtcgccgcg
     1441 ctcattcatc gacggcccgc aggctgcgct gcacttcgcg gccgcgcaaa ctcagccagc
     1501 cggtcacccc atccagcgtc agccccggcg tatcacgccc cgccagccgg cgcgccagcc
     1561 ggaaggcgtc aatgccctgg gcatacagcc gctcggtagc cagcgtcagc ggctcggccg
     1621 gacgcggata gcgcgccgcc ggcgccgctt gcggcgacag cagccacggc atatccaatt
     1681 ggaccacccc cggccaggcc agttgcgggc gggcaaaatc actcaggctg gccgccgcgt
     1741 acgcaggcag cgcgctcagc aaaggacgca aagcaggcca gtcccgcccc ggtacggcca
     1801 gcaccacggc atccgcctgg gcggccagct cgttcaggcg cgcgcgctcg gccggccatt
     1861 gcaccaccca gcgcggcgag cggccatgaa tatccgtcca ggcagcgcgc cacccctgcg
     1921 ccagccgctg cgacagcggg tcagcgccca ccaccagcag cggaaacaaa tgcccggcac
     1981 ggtgcatggc cagcgccatt tgcgcgccct cgctctccac cgccagatga aacgcataca
     2041 aggttggcgc ggcactgcca gacacggtat tcagggccag ggtgggcaca ctggcgattg
     2101 acgccagctc agccaccgcc tcgcgtgcca gcgggccaat caccacgctg gcgccatcgt
     2161 ccagcgcgcg ctgatattgc gcgcgcacat tgtccgcctc ggtgtcgtac cacgccagtt
     2221 cggcgtcggc gtccactttc caggccgctt caatgccctg gcgcagcact tggccagcat
     2281 ccgccagcgg gccgctggcc ggcaacaaca ccgccacgcg cggcgccggg ccggctggcg
     2341 cgctaagctg gccagactgg gccacggcct gcgccacagg cgcaggcaga atggccgtca
     2401 gcggcgagtg cagcgccgcc acatcattca gatcggtgct atagttcggg ccggcggccg
     2461 gcgcggccgc catccatgct gtcagcagcc atccgcacag tatccgcagc caaggagccc
     2521 gtcgttgcat accccactct ttccgattgt cgaagacgcc caagcgtctc tggtgaacgg
     2581 cacattatat gtggtggcca caccgattgg gaacgtagca gacatcagcc tgcgcgcgct
     2641 ggccgtgctg gcccgcgccg acctgatctg cgccgaggat actcgtgtca ccggccagtt
     2701 gctgacgcgc tatggcatcc agagcaaact ggtcagcgtg cgcgagcaca atgaacgcgc
     2761 gatggccgac aaagtctgca actggctggc cgaaggccag ctggtggccc aggtatcgga
     2821 cgccggcacg ccagcagtgt ccgaccctgg cgcgcggctg gtggcggcgg tgcgcgccgc
     2881 cggtcatccg gtgtgcgcca ttcccggcgc ctgcgcggcg gtgaccgcct tggccgccag
     2941 cggcgtcacc accgccgagt ggctgtttca tggttttttg ccacccaagc gcggcgcgcg
     3001 ttgcaagcag ctggaaagct ggcgcgatgc acattatgcc gtggtgctat acgaagcgcc
     3061 gcaccgggtg gccgaatgcg ccgccgacat cgcagtcacg ttgggcgaat cccgcctggt
     3121 gacactggcg cgcgagctga ccaaaacctt tgaaaccgtg cgcaccctgc cggctggcga
     3181 actggcgcag tggattgcca gcgacagcaa tcagcaacgc ggcgagtgcg tgctgatcat
     3241 cgacgccgcc gcccccagtg ccgccgaaac cgccatcgac gcccaggccg aacatacctt
     3301 gcgcgtactg ctggccgagc tgccgcccaa acaagccgcc aagctggctg ccacactgac
     3361 cggcgcgccg cgcgatgcct tgtatcagct ggccgtcagc ctgcgccaca gcgaataatt
     3421 tcgccttggt ttacagcccg agcgcagcaa ggtatgattt gcaccttacc ggaaagcaac
     3481 acctgcccag gtggcgaaat cggtagacgc aggggactca aaatcccccg ccgcaaggcg
     3541 tgtcggttcg agtccgaccc tgggcaccaa caaagcaaca cactcctgca agacattctc
     3601 ggaacccccg tggcacgcca cgggggtttt ttattgccgc cagaaataca aaaacacgcg
     3661 ccgccggcca cccaaactcg cttgggcaaa cggcggcgcg tagctcagcc cattgctgag
     3721 aatcaggcca cgccaatcac cccgccatca tcacgacgaa tcaccaccgt ggccgaacgc
     3781 ggacgcccac ccgccgcgcc atccggccag gcggagacag ccttgggcgc cgccgggtcg
     3841 gaacgctcgg acgaggtttc gcccgggtgc tggatgttga tgaacatggt gcggttatcc
     3901 ggggtaaagg caatgccggt gatttcgcag ccgcgcgggc cagtcagaaa gcggcggaac
     3961 tcgccacgct cgggaatcac cgccagcatc tggttattgc ccatgccagc atagtctttt
     4021 tcgttgagcg tgctggtgga gacatcggtc tgcacccaca gcaggccgtt gctggcgaat
     4081 ttcaggccat cagggctgcc aaaggtgtcg ccctgcacgc tggccttctt gtcgcccttg
     4141 ccggtcgggc tgccgcccag tgcgaacaga tcccagacaa attccgtggt agccgggttg
     4201 ttatcgcctt caaaccagcg aatgatctga ccaaagctgt tgtccacacg cgggttggcg
     4261 gcgtccacgc ccggcttgcc gtccttgccg cgctcgctgt tattggtcag cgtgcagtaa
     4321 atttcatacg gcttgtgcgg gtgggcggcg atccactccg gacggtccat cttggtggcg
     4381 cccaccagat cagcggcacg gcgggtgtgg atgcagatgt cggcctgact ggcaaagcca
     4441 ttggcggcgg tcaggccgtt cttgccgtgc gtcagctcca gccagcggcc ggtgttgtct
     4501 tgatcaaagc gcgccacata cagcgtgccg tgatcgagca agtcgaaatt ggccttgcgg
     4561 tttttcgggt tgtacggctt gctggagaca aatttataga tgtattcaaa gcgctggtcg
     4621 tcgcccatat acaccaccac gcgattgtcc ggcgccagca ccagcatggc gccttcgtgc
     4681 ttgacgcggc ccagcgcggt gcgcttgacc ggggtggatt tggcgtcgaa cgggtcgatt
     4741 tccaccaccc agccaaagcg atgcgcttcg ttcggggtga tctggcggtc aaagcggaaa
     4801 tccgtctccg accatttgta gccggcgtcg gtggccttga tgccgtagcg cttgtcgtca
     4861 gcgctcagtt cgccgttgtt ggcgaagtaa tcgctccagt tttcttcaca ggtcaggtag
     4921 gtgccccacg gcgtctggcc attggcgcag ttgttcagcg tgcccagcac gcgcacgccc
     4981 ttggggtcgt cctgggtttt catcagtgcg tggcccttgg ccgggccgct gatggcgcac
     5041 ggggtggtgg cggtgatgcg acgggccagc gccgacgggc gcaccacgcg ccagcccttg
     5101 ccggtgtctt ccacttcaat caccgacacg cccacggcgg cttgcgcctt ggcggttttc
     5161 ttgtcgttcc agtgggccat gccgtccggg tgcagcaggc catcatcggt gtattcgtgg
     5221 ttgatggcca gcaggccgcg cttgctgtgc gccgagccaa acggcagcgg gaagtaggcc
     5281 atgccgtcgt ggtgcatgcc ggcttgcagc gcctgttctt cggcgctgtt gctggcgtcc
     5341 tgcttgaagg ccggctgctt gcccggcacg ccgaccggat cgccccaggc gtacagcacc
     5401 tgggtggaat agccagccgg cacatgcacc acgtcatccg tcgagaacgg caggccggta
     5461 aagcccaggc tcagcggcgc ggtcggggcc gcattggcgg cctgcgccag ctggctgacc
     5521 gtgccgccag ccaggcccag cgccagcgag gcgcccaggc ccttgagcag accacgacgg
     5581 tcgagcgagg cttcaagaat ggcggaaaac gccgggttgg acgaggcgtt gacgacgctg
     5641 tcgtcggttt tgatcaggcg gctcatgggt gacgctccgg aagtcagcaa cagttgagaa
     5701 cctgtcgaaa atacgtcacg ccgatgacac aatcgtggcg aaaagatgac ttccagatga
     5761 caaagcccgg cacaaggccg ggctttgtca ttacaacagg cagaattaag actcgcgggc
     5821 gacacacctg gcaggcgcat tcaggcgcga cgccccgcct gcgcggtcag acgaggtcag
     5881 ggtttgcagc tcaccggctt catgctgacg ccggctttgc tgagcgcagc gctgcccgct
     5941 tcgtccaggc cgtccagccg cacgctggcg ttggtcaccg ccgtgccgcc gcgcggcgtc
     6001 agtttggccg gatagccgct gtcgttcagg cgcttgacca tggctttggc gctgtcttct
     6061 tgcgagaaca agcccatgga caggccgttt ttgttttcgc cttcggtgtt gacgatgtag
     6121 ttgtcaaaac ccttgtcttt caatgccgcc gattgcttca ccgcctcgtc gcggttggcg
     6181 taagacggca gatacaccca gaacttgccg ccggtgcggc cagtttcctg cacggtttcg
     6241 tgcatcttgg cggccaggcc agccttgttc agcgcagcct tgagcttgct ggcggcggca
     6301 gagtccaggt ccgtcgcctg atagcagacc aacttgggct tggcgtctgg tttggcgtcg
     6361 gcttttttgt cagccacctt ggcgtcggtt ttggcgactt ccggcttggc gggctctggc
     6421 tttttctcgg caggtttgct gtctttcagc tcgaccttgg gctcaggttt tttcacctca
     6481 gccggtttgg cttctgcctt tggttctggc ttggcttccg gttttttcac ttccgccggc
     6541 ttgggctcag gtttgctgac cggagccggc atcggcttgc tggcttcttt cagcggctca
     6601 ggcttgggcg cggcgggttt gtgctcgggg gctttggcag gctccggcgt cggtttggcc
     6661 ggctcaggcg ccagcgccgc cgccagggtg gtcaccggcg gcgtggctgg agcagcaggc
     6721 gctgccgcag gcggtgggtt tttcgcagct tcttgctggg cggcggccaa ggccgagccc
     6781 ggtttgggcg cggtcagcgc ttccagcggc gagggttcat tgccctgccc agccagtttg
     6841 atctgattgg cgttgctttc ccggctcttg atatcagtcg gcgcgggttt actcaaggcg
     6901 cccagcagcg ccaccaccac attggccgcc accaacgcgg ctaaaaacca tttcatcatg
     6961 gtcttgttat cctggctaat ccatccagcg cgagattatc cacaatctgg cagtccagcg
     7021 ggagatgacg cgccactaaa ttggcgtcgc cgccggtcag atacacggtg ggggctggcc
     7081 ccaatgcctg catgcgctgc acgcacagca tgatcggggc caacatggcg ttcagacagc
     7141 cgctataaat agcatcggcg gtggtgtgcg gaaaatcctg atactccccg tccgggcggc
     7201 ccagccgtgc ggtgccctgc gccaggctgt cgcgcatcag ttgcaggcca ggggcaatcg
     7261 tgccgcccag aaacacgcca tccgccgaca aggcatcgac ggtcagcgcg gtgccagccg
     7321 ccgccaccac cttggcgccc ggcgcccgtc cccaggcgcc cagcagcgcc atccagcggt
     7381 cagcgcccag gcgttgcgtg tcgtactgac aggtgacgcc cgcgcactgc gcctgcggcc
     7441 attgccaggc catggccacn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng
     7561 ccacgcccac caccaggctc agcccggaca attggcccac gccgcattca aaacgccaca
     7621 ataaggaaaa agtcaggccg ccgcccaagg gggccagcca gcgccggcca aaacggccgc
     7681 gcccggcggt ctgtgattcc gccagccgcg ccaggccgct ggcctcgcct gcggcggcgc
     7741 gggccagcac gtcgctattg gtcgaggcgg tttcttccac cacttccacc cgcacgccag
     7801 ccggcgcgcc ggcctgaatg gcggcggcgt ccagccagtc aatcggggag agcaaacggt
     7861 agccttgacc gcgcacgcgg tcaatgcgca attgatagcc gtcggccagc gcctgaatgc
     7921 gggcgttgac cgaggcgcgc gaacagccca gctcttcggc caggcgctcg ccagaatgca
     7981 cgccgccatc ggacaggcgg cgcaataccg cccacaccgg agccttcatg gctcagccct
     8041 gcgcgcggat gcggttgagc atggcggtgg tggaggtctc gaactcaaac ggaatcgaat
     8101 gcacctgccc gccgcgcgcc aaggtttcgg cgctgccgac aatgcgttcg atcggccaat
     8161 caccgccttt caccagcacg tccgggcgca ccacgtcaat cagtttggcc ggggtgtctt
     8221 cgtcaaacca ggtcaccagg gacacgcttt ccagcgcggc cattaccgcc gcgcgctggg
     8281 ccagcggatt gatcgggcga tcttcaccct tgccttgccg acgcaccgag gcgtcggtgt
     8341 tgagcgccac caccaggctg gcgcccagcg cgcgcgcttg cgccaaatag gtgacatgcc
     8401 cccgatgcag gatgtcaaaa cagccattgg tcatcaccag cgggcgagcc agggcggaca
     8461 gccgctcggc cagttgttcc ggcgggcaca gcttgcgttc aaattcgggg gcggggtagt
     8521 tcatggcgtt ccatcacgcg gtggcggcaa ccagctcctt gcggtaacgg ttgagttgct
     8581 gcaccgactc aaaagggcgt tcaaacaaac tcgacaagtt gcgaaggata ccacccacca
     8641 cgcgcgattc ccattcggcg gtgaagcgaa tcttgtgggt cagccagtct tccagccatt
     8701 ccgggtcggg caagcggctt tgcacggtgt cgttggggaa cagcgcctga ttgacgtgca
     8761 gattggtcgg atgcagcggc ttgccagagc ggccactggc ggccatcagc atgccgattt
     8821 tggcaaacgc cagccgcgcc tggtcgccaa agcggtcgat ggcctgtttc atatacttga
     8881 ggtaggcgcc gccatggcgg gcttcgtcgc gcgacagggt gctgtagatg tgctgaatca
     8941 ccggctcggt atgccattcc gaggcgcagc gataccactg ggtgaggcgc acttcgccgc
     9001 agaagtgcag catcagggtt tccagcggcg gggcggggtc gaactcaaag cgcacggcgt
     9061 gcagctcttc ttcggtgggg accagttccg ggcggaagcg gcgcaggtat tcgatcaaca
     9121 ccagcgagtg cttttgttct tcgtaaaacc agatcgacat gaacgcagaa aaatcgctgt
     9181 cgtggcgatt gtcgcgcagg aacatctcgg tggccggcaa cgccgaccat tcggtgatgg
     9241 cgttcagacg gatggtttgc gcctgttcgt cagtcagcag ctcggggttg aactgatccc
     9301 acggaatgtc cgtggccatg tcccagcggg ctttttccag ctgggcgaac aactcggggt
     9361 aaagcatcca gaaagtcctt gtgggcgtct cgacaagaac tgcattgtag aagaacacct
     9421 gcggcagcgg aaatgttttc tattcccatt tttgtagtat ggttttgttt tgtaaggaga
     9481 atttcaaaca tccgtcagaa tttcgccatc agcaaaatcg accggatata gacagcccag
     9541 cgcgccgaca gcgccaga
//
LOCUS       DAOPYX010000096         9548 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_8419_length_9548_cov_7.732013, whole genome shotgun sequence.
ACCESSION   DAOPYX010000096 DAOPYX010000000
VERSION     DAOPYX010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 9548)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9548)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9548
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8419_length_9548_cov_7.732013"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(340..918)
                     /locus_tag="PKN55_06505"
     CDS             complement(340..918)
                     /locus_tag="PKN55_06505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome B"
                     /protein_id="HNX70926.1"
                     /translation="MTEADAMQAFSRFRLYHWLVALLFAAAYLTGEDAGLAHVWLGYG
                     LLALLALRLTLALLGRARGFPRLWPQSPRLSGRIGRWVTLGVLSGSLLTLGLGMSMVD
                     NRAALSEGLAMLMPAARADDGGGDDDHHAMRWGDWLGRALRDVEEAHEALSQGALALV
                     AGHIGWVWLTQRRQAVAMLRGVQANQAGAKRS"
     gene            1199..1597
                     /locus_tag="PKN55_06510"
     CDS             1199..1597
                     /locus_tag="PKN55_06510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014088196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1924 domain-containing protein"
                     /protein_id="HNX70927.1"
                     /translation="MRIFWGLAGLCVVTLAHANPALLDNYAREARAADPAFAGFSAER
                     GRALYFRESSASGKPMQCASCHTANPRQPGKTPAFRQIEPLAPVANPMRFTDAKKVEK
                     WFRRNCDDVFRRECSAQEKGDFVRWLSSLP"
     gene            1767..2270
                     /locus_tag="PKN55_06515"
     CDS             1767..2270
                     /locus_tag="PKN55_06515"
                     /inference="COORDINATES: protein motif:HMM:NF021165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diheme cytochrome c"
                     /protein_id="HNX70928.1"
                     /translation="MGKMIWPLALCLAMASAWADDDEHHERHDKRRPRQLSSAGAPAV
                     WKTECGSCHMAYPAGLLPAAAWRQQLDTLRQHYGVNATLDAADMQVIRDYLLRASASN
                     TLPTPASTAGEPPRITRTAWFIRKHDEVAPAVWRRASVKSASNCMACHRDAERGDFDD
                     DRVRIPR"
     gene            complement(2306..2743)
                     /locus_tag="PKN55_06520"
     CDS             complement(2306..2743)
                     /locus_tag="PKN55_06520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011136228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/biocide efflux PACE transporter"
                     /protein_id="HNX70929.1"
                     /translation="MPAHPLPRTLRERVLHALLYELCAMALLAPLGSWLLGHSLAHTG
                     ALALWMSATAMLWNMLFGALFERLEAQLGWTRTPWVRSLHAIGFEGGLVLLCVPVVAW
                     WLQVSYWQALWLDIGVLLFFLPYTWLFNWLYDHARARRAQAVA"
     gene            2827..3681
                     /locus_tag="PKN55_06525"
     CDS             2827..3681
                     /locus_tag="PKN55_06525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012697979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HNX70930.1"
                     /translation="MFASPDALVAFVLAAELGSFSAAARQLGKRQSTISEAIANLEID
                     LGVMLFDRQARLPTLSEAGANLLPLARRILAAQDALSVQARRLADGGEPRLSLVLSDT
                     FPSLEFEAMLHAFAERFPATELECLVGEDADVVALVRSGRAHLGLLTAQPGYPADLAH
                     QRLPLQAQTSLYVAASHPLASRNGCREADLREHRQLRLQTCQAGPADESGQLAWSAPS
                     YLLLLELAQCGFGWAELPHWLAARYGQGLTALALPGWPRQRAMDAVWSRQRPPGPAQA
                     WMLTQLAG"
     gene            complement(3686..5374)
                     /locus_tag="PKN55_06530"
     CDS             complement(3686..5374)
                     /locus_tag="PKN55_06530"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNX70931.1"
                     /translation="MHIALWGVMGIHGAVVLAACGLLWMAGAPGSAWGLAGLGVVGAG
                     GLLACVYPPWLRPLHKLRASLRNRPKQAMAEPWPLPGGALDEAIEGFNHERAALCRVL
                     GGVRQHNIALSIQAAHIGKVIQDARDKALEQERLAEEIFALTQRSQQEAGAMQASVGV
                     IAGFTGALAERAADTCGAVRDADLRAREAVTVMDDFAHTLGQLQEDTRAIIDSVAEIR
                     GISDQTNLLALNAAIEAARAGESGRGFAVVADEVRKLAERTHALAVTVSGKVEAMHTL
                     SGQTASHTQTVVDSMGEASSLLERANQALDGFAQGSRQVNQEIGVVREAIDVLAGNND
                     DIHHHVGRMNQLADDMAGVMQTSATASGELIAAAEQVMRELGRFQLADGPFDQVVRQL
                     YACRDECERKLEALAAAGHNLFDRQYTPIAGTNPQQYHTSYDRAFEQVFRPYFDQTTA
                     SIPGCDLVVLLTGDETYPPTHISKYSQPATDDPAHNLAFARDKRFHRANRMLHRTCTD
                     SGEFLFQAYVRDIGDIYALVSVPVHHRGRHWGGLIFSLRHEALLGGGAPKVSAR"
     assembly_gap    5429..5528
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            5581..6657
                     /locus_tag="PKN55_06535"
     CDS             5581..6657
                     /locus_tag="PKN55_06535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX70932.1"
                     /translation="MISHDPAFCFIDRLLDPARLGVTDTPVGARLHPSQINVNAGQYQ
                     SPYTLAFDFDWATLTHGLEKPPFAHWREQGREPAFARGPHDWFAPSAYARAHAHPAHG
                     PLPRHYPPSRPPAGVERHRHDAWRAQRLLAVAAAMVGYGWRQHLIPDWNPDAAWFARF
                     GLPADPGQGVSGGAYVAWLYNYALGIQLELDVAAQSRARHVHAPTVGRLTIQRVADAR
                     CSYRQLRQQLRPGDVLYLAEGYARQDIRRRLDADDPPPASHALLWLGEVGQAPAGVPL
                     VSDSHAANWLRDTYGSFIPSGVQLRPFYDAAPGSDLRRCCPPGAQSAYFEQFLWASRL
                     LPDVVESEPVGIVEEWLEVATFAG"
     gene            complement(6781..7164)
                     /gene="panD"
                     /locus_tag="PKN55_06540"
     CDS             complement(6781..7164)
                     /gene="panD"
                     /locus_tag="PKN55_06540"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HNX70933.1"
                     /translation="MQRMMLKSKIHRVTTTQCELHYEGSCAIDDDLLEAADIKEYEQI
                     DIWNVTNGERFTTYAIRGERGSGIISVNGSAARRAAVGDILIIATFAAMSDEESRQYA
                     PKLVYVNEENRIKGIGGSIPVQAAE"
     gene            complement(7198..8031)
                     /gene="panC"
                     /locus_tag="PKN55_06545"
     CDS             complement(7198..8031)
                     /gene="panC"
                     /locus_tag="PKN55_06545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008954280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HNX70934.1"
                     /translation="MHILQTIQAVRDWRRQAGRVAFVPTMGNLHDGHLALVREARQRG
                     DAVIVSIFVNRLQFGQGEDFDSYPRTFEADRAKLQAAGVDALFLPDERELYPRIRQDF
                     NVEPPHLQNELCGAFRPGHFRGVATVVTKLFNIVQPDVACFGKKDFQQLAIIRAMVDD
                     LNMPIDIVPVDTGRADDGLALSSRNGYLSADERAEAPRLYRNLRQMHDALAAGSNQYA
                     DLEAAARADLETHGWQVDYIEVRAARTLDIAHAGEHQLVVLAAARLGKTRLIDNIEVQ
                     R"
     gene            complement(8066..8857)
                     /gene="panB"
                     /locus_tag="PKN55_06550"
     CDS             complement(8066..8857)
                     /gene="panB"
                     /locus_tag="PKN55_06550"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011135187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HNX70935.1"
                     /translation="MKITVQTLMKLAQEGQKITMLTCYDASFAATLDEAGVETLLVGD
                     SLGMVMQGADSTLPVSLEEMAYHVRCVARGARNALIIGDMPFGSYQESPQQAFASAAK
                     LMQAGAHMVKLEGGAFMADTTRFLVERGIPVCSHIGLTPQYVNTFGGYRVQGKSEDDA
                     ARIRADARALADAGASMVLMECVPAALAGAITAELPVPTIGIGAGPEVSGQVLVIYDM
                     LGVYPGKKARFVKNFMPEAGSIAGAVKAYVHAVKNGSFPAAEHCF"
     gene            complement(8970..>9548)
                     /locus_tag="PKN55_06555"
     CDS             complement(8970..>9548)
                     /locus_tag="PKN55_06555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008954278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HNX70936.1"
                     /translation="LARKLAEHCQLRLCADPAHANPFLPRFYQNMNHHALATQLYCLR
                     ERAKLARELGAESCANGCVRDFLFEKDDLFARLTLQGEELALYQSLQAALLPAELPAP
                     DLVVCLQASTEVLARRIAARGDAHTVAFPDGYLKRMNAAYNEFFMQYEAAPVVMVNTE
                     RLDFVDKPEDFELLLRCISEAKGQRSYFNVAV"
BASE COUNT         1570 a         3085 c         3094 g         1699 t
ORIGIN      
        1 tctactttgc caagctgttc ccggaagtgc acagcctgct ggtcaccctg aaagctggcg
       61 ccaagccgct gatggagctg gacgaagcgc actttgcttt ctgatattgc atgacatcgc
      121 cgcgcgcctt ggcgcgcggc atatgaagtg tgtaggtgct gttgtggcag atatgccctg
      181 acgccgcgac ccgcgagggt ccggcgtttt tttatctgct tttgtcgcag ccgggctggg
      241 ctgtgtctgc tgattcaaga ctcagcgcag cggctctgcg cgagctgtgt ggccgcagac
      301 agtacggcaa ggccgtgcaa caacgccagg agggttgggc tagctgcgct tggcacccgc
      361 ctggtttgct tgcaccccgc gcagcatggc caccgcctga cggcgctggg taagccacac
      421 ccagccgata tgcccggcca ccagcgccag cgcgccttgc gacagcgcct cgtgcgcttc
      481 ttccacatcg cgcagtgcgc ggcccagcca gtcgccccag cgcatggcgt ggtgatcgtc
      541 atcgccgccg ccgtcatcgg cgcgcgccgc cggcatcaac atggccagcc cttcgctgag
      601 cgccgcgcgg ttgtccacca tgctcatgcc cagccccagc gtcaacaacg accccgacaa
      661 cacccccagc gtcacccagc ggccaatccg cccgctcaga cgtgggcttt gcggccacaa
      721 acgcggaaaa ccgcgcgccc ggcccagcag cgccagcgtc agccgcagcg ccagcaaggc
      781 cagcaggcca tagcccagcc agacatgcgc cagcccggcg tcctcgccgg tgaggtaagc
      841 ggcggcgaac aacagcgcca ccagccagtg atacagacga aaacgggaaa atgcttgcat
      901 ggcgtcagcc tcagtcatgg tgaatgccgc catcttggcc ccggcaactt aactgaacat
      961 gaaaccttgt ccgccatagg gttaatcccg gcttaagctt ggcccggcat gctatgacgc
     1021 aaccatgttt ggactgaaac cagcgacaag cgccgctcac acaacccgcc tggcgttgtt
     1081 gcccgacctt gccgtacttc ttgtactgtc tacggccgtg cgcctcgcca ggcgcgctgc
     1141 gctgggtttt gtgagcaacg ctaagcgtgg gtttctgtgt tactgaggag ttgatgctat
     1201 gcgtatcttt tgggggctgg ccggcctgtg cgtcgtcacg ctggcccatg ccaatcccgc
     1261 cttgctggac aactacgccc gtgaagcgcg cgccgctgat ccggcctttg ccgggttttc
     1321 cgccgagcgc ggccgcgcct tgtattttcg ggaatccagc gccagcggca agccgatgca
     1381 atgtgcgagc tgccacaccg ccaacccgcg ccagccgggc aaaaccccgg cttttcgcca
     1441 gatcgagccg ctggcgccag tggccaaccc gatgcgcttc actgacgcca agaaagtgga
     1501 gaaatggttt cggcgcaatt gcgacgatgt gttccgccgc gaatgcagcg cgcaggagaa
     1561 gggcgacttt gtgcgctggc tgagcagctt gccataagag ccgctcacaa aaccctcctg
     1621 gcgttgttgc ccgaccttgc cgtactacct gtactgtcta cggtcgtgcg cctagccagg
     1681 atcgcttcgc tgagttttgt gagcgacttt aagcgccgct cacaaccccc ctggctttgt
     1741 gagccgcact gaatctggag aaatatatgg gcaaaatgat ctggccgctg gcgctatgcc
     1801 tggccatggc atcagcatgg gcggacgacg acgaacacca cgaacgtcac gacaagcgcc
     1861 gtccgcgcca actgagcagc gccggcgcgc cggcagtatg gaaaaccgaa tgcggcagct
     1921 gccatatggc ctacccggcc ggcctgctgc ctgctgccgc ctggcggcaa cagctggaca
     1981 ccctgcgcca gcactacggc gtcaacgcca cgctggacgc cgccgatatg caggtgatcc
     2041 gcgactatct attgcgcgcc agcgccagca acaccctgcc tacgccagcc agcacggcgg
     2101 gcgagccgcc gcgcatcacc cgcaccgcct ggtttatccg caaacacgat gaagtcgccc
     2161 cggcggtgtg gcggcgcgcc tcggtgaaaa gcgccagcaa ctgcatggcc tgccaccgcg
     2221 acgccgagcg cggcgatttt gacgatgacc gggtgcgtat tccgcgctga gaccgtcaat
     2281 catcaccatg ttgcatgggg gggctttacg ccacggcctg cgcacgccgt gcgcgggcgt
     2341 ggtcatacag ccagttgaac agccaggtat acggcaggaa aaacagcagc acgccaatat
     2401 ccagccacag cgcctgccag tagctgactt gcagccacca ggccaccacg ggcacgcaca
     2461 gcagcaccag accgccttca aaaccgatgg catgcaggct gcgcacccat ggcgtgcgcg
     2521 tccagcccaa ttgcgcttcc agccgttcga acagtgcgcc aaacagcata ttccacagca
     2581 tggcggtggc cgacatccac agcgccagtg cgcccgtgtg cgccaggctg tggcccagca
     2641 gccagctgcc cagcggagcc agaagcgcca tggcgcacag ttcgtataac agcgcgtgca
     2701 acacgcgctc gcgcagcgtg cgcggcaagg gatgggcagg catggcgact ccataggcaa
     2761 aaaactgccg ccagtgtgct aggcttttac cgggtgttca atgtaacttc catcggataa
     2821 atcgatatgt ttgcctctcc tgatgccttg gtggcgtttg tgctggcggc ggagctgggc
     2881 tcgttttcgg cggcagcgcg ccagttgggc aagcgccagt ccaccatcag cgaggccatt
     2941 gccaatctgg aaattgacct gggcgtgatg ttgtttgacc gccaggcacg tttgccgaca
     3001 ctcagtgagg ccggcgccaa tctgctgccg ctggcccggc gaattctggc cgcgcaggat
     3061 gccctgagcg tgcaggcgcg ccggctggcc gacggcggcg agccgcgcct gagcctggtg
     3121 ctgtcggata cctttcccag cctggaattt gaagccatgc tgcacgcctt tgccgagcgc
     3181 tttcccgcca ccgagctgga atgcctggtg ggcgaagatg ccgacgtggt ggccttggtg
     3241 cgcagtggcc gcgcccattt gggtttgctg accgctcagc cgggctaccc ggccgatctg
     3301 gcgcatcagc gtttgccgct gcaggcgcaa acctcgctgt atgtggccgc cagccatccc
     3361 ttggccagcc ggaatggctg tcgtgaggcg gatttgcgcg agcatcgtca gctgcgcctg
     3421 caaacctgcc aggctggccc ggcggacgag tcggggcaat tggcctggtc ggcgccgagt
     3481 tatttgctgt tgctggagct ggcgcagtgc gggtttggct gggctgagct gccgcactgg
     3541 ctggccgcac gttatggcca ggggctgaca gcgctggcgc tgccgggctg gccacgtcag
     3601 cgggccatgg atgcggtatg gtcacgccag cgcccgcccg gcccggcgca ggcatggatg
     3661 ctgacgcaac tggcagggta aggctttacc gggcactgac cttgggcgcg ccgccgccca
     3721 gcagcgcttc gtggcgcagg ctgaaaatca gcccgcccca gtggcggccg cgatggtgca
     3781 ccggcactga caccagcgcg taaatatcgc caatatcacg cacataagcc tggaacaaaa
     3841 actccccgct gtcggtgcag gtgcggtgca gcatgcggtt ggcgcggtga aagcgtttgt
     3901 cgcgggcaaa ggccaggttg tgcgccgggt cgtcggtggc cggctggctg tatttgctga
     3961 tgtgggtggg cggataggtt tcatcgccgg tcagcagcac caccaggtcg cagccgggaa
     4021 tgctggcggt ggtctggtcg aaatacggcc ggaaaacctg ctcgaaggcg cggtcgtagc
     4081 tggtatggta ttgctgcggg ttggtgccgg caatcggcgt gtactggcgg tcgaacagat
     4141 tatggccagc ggcggccagc gcttccagct tgcgctcgca ttcgtcgcgg caggcgtaca
     4201 gctggcgcac cacctggtca aacggcccat cggccaactg aaagcgcccc agctcgcgca
     4261 tcacctgctc ggcggcggca atcagctcgc ccgaggcggt ggcgctggtt tgcatcaccc
     4321 cggccatgtc gtcggccagt tggttcatgc ggccaacgtg gtggtggatg tcgtcattat
     4381 tgccggccag cacgtcgatg gcctcgcgca ccacgccgat ttcctggttg acctgacggc
     4441 tgccctgggc aaagccatcc agcgcctggt tggcgcgctc cagcaggctg ctggcctcgc
     4501 ccatgctgtc caccacggtt tgcgtgtggc tggcggtttg cccggacagg gtgtgcatgg
     4561 cttcaacctt gccgctcacc gtcaccgcca gcgcatgggt gcgctcggcc agtttgcgca
     4621 cttcgtcggc caccacggca aaccctcggc cggattcgcc agcgcgggcg gcttcgatgg
     4681 cggcgttcag cgccagcagg ttagtctgat ctgaaatccc gcgaatttcc gccacgctgt
     4741 cgatgatggc gcgggtgtct tcctgaagct ggcccagggt gtgggcaaag tcgtccatca
     4801 ccgtcaccgc ctcgcgggcg cgcaagtcgg cgtcgcgcac cgcgccgcag gtgtcggcgg
     4861 cgcgctcagc cagcgcgccg gtaaagccgg cgatcacgcc gacgctggct tgcatggcgc
     4921 ccgcttcctg ctggctgcgc tgggtgaggg caaagatttc ttcggccagg cgttcttgct
     4981 ccagcgcctt gtcgcgcgcg tcctgaatca ccttgccgat gtgcgccgcc tggatggaca
     5041 gcgcgatatt gtgctggcgc acgccgccca gcacccggca cagcgcggcg cgctcgtggt
     5101 taaagccctc gatggcttcg tccagcgcgc cgccaggcag cggccagggt tcggccatgg
     5161 cttgcttggg ccggttgcgc aggctggcgc gcagcttgtg cagcggccgc agccacggcg
     5221 gatacacaca ggccagcagc ccgccggcgc ccaccacacc caaaccggcc agcccccagg
     5281 ccgagcccgg tgcgccagcc atccacaaca ggccgcaggc ggccagcacc acggcgccat
     5341 gtatgcccat gacgccccat aacgcgatat gcatgatttg tcttcctcta tccgggtctg
     5401 cgcccgtttg cctgaatggc ggacgcatnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5521 nnnnnnnngc cccacagcgt tgtgtcaggc agaaccgaca ttgcccgctc aggagccgcc
     5581 atgatctcgc atgaccccgc tttttgcttt atcgaccgcc tgctggaccc ggcgcggctg
     5641 ggggtgaccg acaccccggt cggcgcccgg ctgcacccca gccagatcaa tgtcaacgcc
     5701 ggccagtatc aatcgccgta caccctggct tttgattttg actgggccac gctcacccac
     5761 ggcctggaaa aaccgccgtt tgcccattgg cgcgagcaag gccgcgagcc ggcgtttgcg
     5821 cgcggcccgc acgactggtt tgccccgtcc gcctatgccc gcgcccatgc ccatccggca
     5881 catggtccgc tgccgcgcca ctatccgccc agccgcccgc ccgccggcgt tgagcgccat
     5941 cgccacgacg cttggcgcgc gcagcggctg ctggcggtgg cggccgccat ggtgggctat
     6001 ggctggcgcc agcatctgat cccggactgg aaccccgacg ccgcctggtt tgcccgcttt
     6061 ggcttgcccg ccgaccccgg ccagggtgtg agcggcggcg cctatgtggc ctggctgtac
     6121 aactacgcgc tgggcattca gctggagctg gatgtcgccg cgcaaagccg cgcccgccat
     6181 gtgcacgcgc caaccgtcgg ccggctgacg attcagcggg tggccgacgc gcgttgcagc
     6241 taccggcagt tgcgtcaaca actgcgcccc ggtgatgtgc tgtacctggc cgagggctac
     6301 gcccgccagg acatccgccg ccggctggac gccgacgatc cgccgccggc cagccatgcg
     6361 ctgctgtggc tgggcgaagt gggccaggcg ccggccgggg ttcccttggt cagcgacagc
     6421 cacgccgcca attggctgcg cgacacctat ggcagtttta ttccgtccgg ggtgcagctg
     6481 cggccgtttt atgacgccgc gccgggcagc gacttgcgcc gctgctgccc gccgggcgcg
     6541 caaagcgcgt atttcgagca gtttttatgg gccagccgcc tgctgcccga cgtggtggag
     6601 agtgagccgg tggggattgt cgaggagtgg ctggaagtgg cgacgtttgc cggctgagca
     6661 tggcgctcgc tgagcgagcg gcgccaacac cgccaaaagc atgcggtgag cggcaaaaac
     6721 gccgggggct ggccagatca acactggcca gcccccggcg catcaggcgc gtgcgcgaaa
     6781 ttactcggcg gcctgcaccg gaatcgaacc gccaatgccc ttgatgcggt tttcttcgtt
     6841 cacatacacc agcttgggcg cgtactggcg gctttcttcg tcgctcatgg cggcaaaggt
     6901 ggcgatgatc aggatatcgc ccacggcggc gcggcgggcg gccgagccgt tcaccgagat
     6961 gatgccgctg ccgcgttcgc cacggatggc gtaggtggtg aagcgctcgc cattggtgac
     7021 gttccagatg tcgatctgtt cgtattcctt gatgtcggcg gcttccagca aatcatcgtc
     7081 gatggcgcac gagccttcgt agtgcagttc gcactgggtg gtggtgacgc gatgaatttt
     7141 ggatttgagc atcatgcgtt gcatggtcgg ggggtcctct gcgtggggcg ggcagcgtca
     7201 gcgctgcact tcgatattgt cgatcagccg ggttttgccc aggcgcgcgg cggccagcac
     7261 caccaactgg tgttcgccgg cgtgggcgat gtccagcgtg cgggcggcgc gcacttcgat
     7321 atagtccacc tgccagccgt gggtttccag gtcggcgcgg gcggcggctt ccaggtcggc
     7381 gtactgattg ctgccggcgg ccagcgcgtc gtgcatctgg cgcagattgc ggtacaggcg
     7441 cggcgcttcg gcgcgttcgt cggcgctcag gtagccattg cgcgacgaca gcgccaggcc
     7501 gtcgtcagcg cggccggtgt ccaccggcac gatgtcgatc ggcatgttca ggtcatcgac
     7561 catggcgcgg atgatggcca gttgctgaaa gtcttttttg ccaaaacagg cgacgtccgg
     7621 ctgaacaata ttgaacagct tggtgaccac ggtggccacg ccacggaagt ggccaggacg
     7681 gaacgcgccg cacagctcgt tttgcagatg cggcggctcg acgttgaaat cctggcggat
     7741 gcgcgggtaa agctcgcgct cgtcgggcag gaacagcgcg tccacgccag cggcttgcag
     7801 cttggcgcgg tcggcctcaa aggtgcgcgg gtagctgtca aagtcttcgc cctggccaaa
     7861 ttgcaggcgg ttgacaaaga tgctgacaat caccgcgtcg ccgcgctggc gggcttcgcg
     7921 caccagcgca agatggccgt cgtgcagatt gcccatggtg ggcacaaaag ccacgcggcc
     7981 ggcctggcgg cgccaatcgc gcaccgcctg gatggtttga agaatatgca taatgaaatc
     8041 cgggatgggg gctggccggc aaaaatcaga aacagtgctc ggcggcgggg aagctgccgt
     8101 tcttcaccgc gtgcacatac gccttgaccg cgccggcgat gctgccggct tcgggcatga
     8161 agtttttcac aaaacgggct tttttgccgg ggtagacgcc cagcatgtcg taaatcacca
     8221 gcacctggcc gctgacctcg gggccggcgc caatgccgat ggtgggcacc ggcagttcgg
     8281 cggtgatggc gccggccagc gcggccggca cgcattccat cagcaccatg ctggcgccgg
     8341 cgtcggccag cgcgcgggcg tcggcgcgaa tgcgcgcggc gtcgtcctcg ctcttgcctt
     8401 gcacgcggta gccgccaaag gtgttgacgt attgcggggt caggccgatg tgcgagcaca
     8461 ccgggatgcc gcgctcaacc aaaaagcggg tggtgtcggc catgaaggcg ccgccttcga
     8521 gcttgaccat gtgcgcgccg gcctgcatca gcttggcggc gctggcgaag gcctgctgcg
     8581 ggctttcctg gtagctgcca aacggcatgt cgccgatgat cagcgcgttg cgcgcgccgc
     8641 gcgccacgca gcggacgtga taggccattt cttccaggct gaccggcagg gttgagtccg
     8701 cgccttgcat caccatgccc agcgaatcgc ccaccagcag ggtttccacc ccggcttcat
     8761 caagcgtggc ggcaaagctg gcgtcgtaac aggtcagcat ggtgattttt tgcccttcct
     8821 gggccagttt catcaaggtt tggacggtga ttttcatcag gtgagcttcc ttcatgggac
     8881 agcggcgtcg ccttgggccg gggtctgccg gccgctggcg gtgtgccgcg agggatcatt
     8941 ggccggatgg gccaatggcg ggcctggggt cagacagcga cattaaaata gctgcgttga
     9001 cccttggcct cgctgatgca gcgcaacaat agctcgaagt cttccggctt atcaacaaaa
     9061 tccagtcgtt cggtgttgac catgactacc ggcgcagctt cgtattgcat gaaaaattcg
     9121 ttgtaggcgg cgttcatgcg cttgaggtag ccatccggga aggccaccgt gtgcgcatcg
     9181 ccgcgcgcgg caatgcgccg cgccaggact tccgtcgagg cttgcagaca caccaccaga
     9241 tcgggggccg gcagttcggc gggcagcagc gcggcttgca aggattggta caaggccagc
     9301 tcttcgcctt gcagcgtcag ccgggcgaac agatcgtcct tttcaaacag aaaatcgcgc
     9361 acgcacccat tggcgcaact ttctgcgccc agctcgcgcg ccagcttggc gcgctcacgc
     9421 aggcaataca gctgggtggc cagcgcatga tggttcatgt tctggtaaaa gcgcggcaga
     9481 aaaggattgg cgtgcgccgg gtcggcgcac aggcgcaatt gacagtgttc ggcaagtttg
     9541 cgcgccag
//
LOCUS       DAOPYX010000097         9513 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_8468_length_9513_cov_12.345422, whole genome shotgun sequence.
ACCESSION   DAOPYX010000097 DAOPYX010000000
VERSION     DAOPYX010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 9513)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9513)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9513
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8468_length_9513_cov_12.345422"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..612
                     /locus_tag="PKN55_06560"
     CDS             <1..612
                     /locus_tag="PKN55_06560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011137924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNX70937.1"
                     /translation="RYSLYAPIYDLFAGRFARQRQRSLRLAGPGAQDRVLLVGAGTGL
                     DLPELRACGEVLATDISPAMLARLSRRAARLGMTVKAQVMDAEALDAPDGHYTLVVLH
                     LILAVVDDPIRTLREVERVLAPGGRVAVFDKFLPDGASPPGWRRALNVPLRWLATDIN
                     RRFADIVRYTQLRVAHDEPALWGGTFRVIQLVKPSDSAHHARS"
     gene            609..1391
                     /gene="bioH"
                     /locus_tag="PKN55_06565"
     CDS             609..1391
                     /gene="bioH"
                     /locus_tag="PKN55_06565"
                     /EC_number="3.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014085596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HNX70938.1"
                     /translation="MNLSSLPSLYFESVGRGPDVVLVHGWGLHGGVWSRVAQALADEF
                     TVHCVDLPGHGHSASLQDYTLDNLARALAATFPLPVQVVGWSLGGLAAMRWALDAPAS
                     LKSLTLVASSPCFVQRADWPHAQAVQVMRQFADNLGGQFEQTLRRFLALQTLGSESAH
                     AVLAEMKHILFAHGRPSALEAALAVLENSDVRHELPQLHLPTHLSYGQRDALTPIGAA
                     RWLAGAIAGSTLTEFPQASHAPFLSHEAEFVADLIPFLQRHA"
     gene            1412..2320
                     /gene="bioC"
                     /locus_tag="PKN55_06570"
     CDS             1412..2320
                     /gene="bioC"
                     /locus_tag="PKN55_06570"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018746974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HNX70939.1"
                     /translation="MTEPFYTDKSRVRDAFDRAAHSYDGAAVLQREVSDRMGERLDYI
                     RHAPQRILDAGSGTGYGAVGLRSRYPDAQVIELDLAPSMLRVSRDKQASAPRGGLLGR
                     LFRRAEPWQLCADIERLPLAAGSVDMIWSNLAIQWVNTPDAVFAEFHRALRVDGLLMF
                     STLGPDTLNELNRAFAGVDRHTHVNRFIDMHDIGDALVRAGFATPVMDMEKIVLTYDD
                     IKAVMHDLKAIGAHNVTHGRKTGLMGKTAWRDISARYDAMRRDGKLPATYEVVYGHAW
                     KPAQRPSRKLDDGSQVIEFQPRPNLS"
     gene            2323..3183
                     /locus_tag="PKN55_06575"
     CDS             2323..3183
                     /locus_tag="PKN55_06575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007341878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HNX70940.1"
                     /translation="MKPVCVARRRLLLGALTLALAACASTPPPAQPARPKVALALGGG
                     AAKGFAHVGVIKVLESHGIRPDIITGTSAGAVVGSLYAAGYSGIQLQNMALALDPASI
                     SDLVLGDGGFVKGEKLERYINQQVRNLPIEKLTRPFGAVATDLANGNRVVFRRGNTGQ
                     AVRASAAVPAVFEPVLIQGRKYVDGGLVSPVPVNAAREMGADIVIAVDISTKPNAKGG
                     GSVLGALDQTLNIMGQKLGQAELQHATVVIRPDISRIGSTDFAQKHQAILEGEKAAQA
                     ALPAIQRALK"
     gene            complement(3311..3526)
                     /locus_tag="PKN55_06580"
     CDS             complement(3311..3526)
                     /locus_tag="PKN55_06580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014085527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HNX70941.1"
                     /translation="MFPEYRDLITRLKTEDAHFTRLFDKHNDLDQRIKNMEARIEPAS
                     HEEIETLKKEKLHLKDELYTILRTAAA"
     gene            complement(3702..3881)
                     /locus_tag="PKN55_06585"
     CDS             complement(3702..3881)
                     /locus_tag="PKN55_06585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX70942.1"
                     /translation="MQHNSQERIMTEQATPIAHDSHDSEQDTLQFPYTITEKLQDTLR
                     QALDSAESPRDLGVA"
     gene            complement(4095..5930)
                     /locus_tag="PKN55_06590"
     CDS             complement(4095..5930)
                     /locus_tag="PKN55_06590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012695555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNX70943.1"
                     /translation="MKDQQLSASYQTLVNRTNPKLMTHDDQNPYSSKLILIIDSVPEM
                     QRALTMTLASFGANKVEFATRPGDAMAKLARYSFDIVLCDFDLGNGYDGLHLLEEAKT
                     RNLLKQSCVFMIVSGERRSQRVLAAAELEPDGYLIKPFTGEQLRVRLDRAMRRREAFR
                     CVDDALKQNDLLVAINSCNDRISQGDEFALDFMRLRGSLSLKIGDYLSAKSLYEEVMA
                     IKAQPWAQLGLAKSLTQLKQYDEAKVMFDEVLSKNGRVMEAYDWLAKLYEAQNDMSAA
                     QETLENAVQLSPLAIRRQKELAEVAVQNHDYDTAERALKKTIDVAKYSVYRAVEDYAA
                     LARVQIAKGDLQSANQTAGTVRREFRHNAVAEWMATIIDSQVFKKQGQPHRGRELLDE
                     AIKRMPELEEKLSQQAKMELIQACYAQGREDVGNSMVQQIVRNNHDNQHVLERLTRIY
                     EQVDRLEVGKQLIAENVQSVVDINNQAVRLAQSGQLEDAVQMFMQAVEELPQNQQILL
                     NAVNAILAMVHRKGWHETHMAHAYHFLERVRQMDALNGKYQKLQHSYRQVIEQHGKQQ
                     WTPVLPKTEEELAAEAAAAEEAAAQAAALKANKGKPVPAPGKTVS"
     gene            complement(5982..8144)
                     /locus_tag="PKN55_06595"
     CDS             complement(5982..8144)
                     /locus_tag="PKN55_06595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012695556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HNX70944.1"
                     /translation="MFSDHLAGLNPEQLRAVTLGAGSALVLAGAGSGKTRVLTTRIAW
                     LIANQRAMAANIMAVTFTNKAAREMHTRLSAMLPLNPRGMWIGTFHGLANRFLRAHWR
                     DAGLPQTFTILDMQDQLAAIKRLMKALQVSDEKFPPRQVQAFINNHKEQGLRAEALKP
                     YDAFNAKLTELYAAYDAQCRREGVVDFAELLLRSYEMLNSNEALREHYRARFSHILVD
                     EFQDTNKLQYAWLCLFAGPHASVFAVGDDDQSIYAFRGAEVGNMRRLMADFQVGEPIK
                     LEQNYRSCGNILDAANALIQHNDGRLGKNLWTDAGAGEPIRLYRGWNDADEAQFIVDE
                     VRALRRDGGALAEMAVLYRSNAQSRVLEHALFQAGLPYRVYGGLRFFERQEIKHALAY
                     LRLVASPDDDNALLRVINVPARGIGARTVENLQTASRERGVSLWQAACGQGGGRSAAS
                     LGKFVLLIEQLRADAERLSFAQLVSHLIAASGLRAMYEADKDGEDRLENLDELISAAV
                     SFAPEDPAQIIAEFLAHASLEAGEHQAGAGEDALQLMTVHAAKGLEFASVFVSGLEEG
                     LFPHENSLNDAHGVEEERRLMYVAITRARRRLYLTQAQQRMLHGQTRYPLPSRFMDEI
                     PESLTKNLSQTMTAGLSSRSERSTRPAPASAPRHQASANTALPGGWRIGQSVTHPKFG
                     SGVILNAEALGEELRLYVNFAEHGAKWLDMRFAKLTPA"
     gene            8437..9129
                     /locus_tag="PKN55_06600"
     CDS             8437..9129
                     /locus_tag="PKN55_06600"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HNX70945.1"
                     /translation="MAYFTLELQDSVYVLTMINGDKGNVMDDAWLDELTVHLDAIEAD
                     PAANRALVLASNHEKAWSNGIDLDYIRSKGMAYLFDHFVPRLDGFLGRIAWLNLPTVA
                     CISGNAYGGGALIASACDFRTMRADRGRICFPEIDLKLGLSPAMVECVNTLPNEQARW
                     EMVMTGRALGGEEAAARQIVDRALSAEALLPETLALAAQLASKDRVAYTRLKRLLRPR
                     WAKFAPQAKSAA"
     gene            complement(9130..>9513)
                     /locus_tag="PKN55_06605"
     CDS             complement(9130..>9513)
                     /locus_tag="PKN55_06605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012695577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction DNA helicase RuvB C-terminal
                     domain-containing protein"
                     /protein_id="HNX70946.1"
                     /translation="IARRARGTPRIANRLLRRVRDFAEVRSDGRVSREIADAALSMLD
                     VDHAGLDVMDRKLLSAIIEKFSGGPVGLDNVAAAIGESADTIEDVLEPYLIQQGLLQR
                     TPRGRMATVLAYQHFGLSAPERLPG"
BASE COUNT         1584 a         2980 c         3072 g         1877 t
ORIGIN      
        1 cgctactcgc tctacgcacc gatttacgat ctgtttgccg gccgttttgc ccgtcagcgc
       61 cagcgttcgc tgcggctggc cgggccgggg gcgcaggaca gggtgttgct ggtgggggcg
      121 ggcaccgggc tggatttgcc cgagctgcgc gcctgtggcg aggtgctggc cacggatatt
      181 tcgccggcca tgctggcgcg cttgtcgcgg cgggcggcgc ggttggggat gacggtcaag
      241 gcgcaggtga tggacgccga ggcgctggat gcgccggacg gtcattacac cctggtggtg
      301 ctgcacttga ttctggctgt ggtggacgac ccgatccgca ccttgcgcga ggtggagcgg
      361 gtgctggcgc cgggtgggcg ggtggcggtg tttgacaaat tcttgcccga cggcgccagc
      421 ccgcccggct ggcggcgggc gctgaacgtg cccttgcgct ggctggccac cgacatcaat
      481 cgccgctttg ccgatatcgt gcgctacacc cagctgcgcg tggcgcatga cgagccggcg
      541 ctgtggggcg gaacctttcg ggtgattcaa ctggtcaaac cgtccgattc tgcgcatcat
      601 gcacggtcat gaacctgtct tctttgcctt ctttgtattt tgagagcgtc gggcgcggcc
      661 cggatgtggt gctggtgcac ggctggggcc tgcacggcgg ggtgtggtcg cgggtggcgc
      721 aggcgctggc cgacgagttc accgtgcatt gtgtggactt gcccggccac ggccacagcg
      781 ccagcctgca agactacacg ctggacaatc tggcgcgcgc gctggcggcg acgtttccgc
      841 tgccggtgca ggtggtgggc tggtcgctgg gcggcctggc cgccatgcgc tgggcgctgg
      901 atgcgccggc ctcgctgaaa agcctgaccc tggtggcctc cagcccgtgc tttgtgcagc
      961 gcgccgactg gccgcacgcg caggcggtgc aggtgatgcg ccagtttgcc gacaatctgg
     1021 gcggccagtt tgagcaaacc ttgcgccgtt ttctggccct gcaaacgctg ggcagcgagt
     1081 cggcgcacgc cgtgctggcc gagatgaagc acatcctgtt cgcccacggc cgccccagcg
     1141 cgctggaagc ggcgctggcg gtgctggaaa acagcgatgt gcgccacgag ctgccgcaac
     1201 tgcatctgcc cacccacctc agctacggcc agcgcgatgc gctgacgccg attggcgcgg
     1261 cgcgctggct ggccggcgcc attgccggca gcacgctgac cgaattcccg caggcctcgc
     1321 atgcgccgtt cttgtcgcac gaggcggagt ttgtggccga tttgatcccc ttcttgcagc
     1381 gccatgcgta aagcctggcg ttggaaccgt gatgaccgaa ccgttttaca ctgataaatc
     1441 ccgtgtgcgc gacgcctttg accgcgccgc ccacagctat gacggcgctg ccgtgttgca
     1501 gcgcgaagtg tccgaccgca tgggcgagcg gctggattac atccgccacg ccccgcagcg
     1561 cattctggac gccggcagcg gcaccggcta cggcgcggtg ggcctgcgca gccgctaccc
     1621 ggacgcccag gtgatcgagc tggatctggc gccgtcgatg ctgcgcgtgt cgcgcgacaa
     1681 acaagccagc gcgccgcgcg gcggcctgct ggggcgcttg ttccgccgcg ccgagccgtg
     1741 gcagctgtgc gccgacatcg agcgcctgcc gctggccgcc ggcagcgtgg acatgatctg
     1801 gtccaatctg gccatccagt gggtgaacac cccggacgcg gtgtttgccg agtttcaccg
     1861 cgcgctgcgg gtggacggcc tgttgatgtt ttccaccctg gggccggaca cgctcaacga
     1921 gctgaaccgc gcctttgccg gcgtggaccg ccacacccac gtcaaccgct ttatcgacat
     1981 gcacgacatc ggcgacgcgc tggtgcgcgc cggctttgcc accccggtga tggacatgga
     2041 aaaaatcgtc ctcacctacg acgacatcaa agcggtgatg cacgacctga aagccatcgg
     2101 cgcgcacaat gtcacccacg gccgcaaaac cgggctgatg ggcaaaaccg cctggcgcga
     2161 catcagcgcc cgttacgacg ccatgcgccg cgacggcaaa ctgccggcca cctacgaagt
     2221 ggtgtacggc cacgcctgga agccggcgca acgccccagc cgcaagctgg acgacggcag
     2281 ccaggtgatt gaatttcaac cccgtccgaa tttgtcctga tcatgaagcc tgtttgtgtt
     2341 gcccgtcgcc gcctgctgct gggcgcgctg actttggcgc tggccgcctg cgccagcacg
     2401 ccgccgcccg cccaacccgc ccgccccaaa gtcgccctgg cgctgggcgg cggcgcggcc
     2461 aagggctttg cccacgttgg ggtgatcaag gtgctggaat cacacggcat ccgcccggac
     2521 atcatcaccg gcaccagcgc cggcgcagtg gtgggcagcc tgtacgccgc cggctacagc
     2581 ggcatccagc tgcaaaacat ggcgctggcg ctggacccgg cgtcgatcag cgatttggtg
     2641 ctgggcgacg gcggctttgt gaaaggcgaa aagctggagc gctatatcaa tcagcaggtg
     2701 cgcaatctgc cgattgaaaa actgacgcgg ccgtttggcg cggtggccac cgatctggcc
     2761 aacggcaacc gcgtggtgtt ccgccgtggc aacaccggcc aggcggtgcg cgcctcggcc
     2821 gccgtgccgg cggtgttcga gccggtgttg attcaggggc gcaaatatgt cgatggcggc
     2881 ctggtcagcc cggtgccggt gaacgccgcg cgcgagatgg gcgcggacat cgtgatcgcc
     2941 gtggatattt ccaccaagcc caacgccaag ggcggcggca gcgtgctggg cgcgctggac
     3001 caaacgctga acatcatggg ccagaagctg ggccaggccg aactgcaaca cgccaccgtg
     3061 gtgattcgcc cggatatctc ccgcattggc tcgaccgact tcgcccagaa acaccaggcg
     3121 attctggaag gcgaaaaagc cgcccaagcc gctttgccgg ccattcagcg cgcgctgaag
     3181 taaccttgtt ctggcgcggg cgcaagccac ggcacgcatg gcggtgagcc cgctatcccg
     3241 cagcagataa cacaaggccc gccgacgtga ccgtcaggcg ggccttgtgt tatcgggctg
     3301 ggccgatcaa tcaggcagcg gccgtgcgca ggatggtgta cagctcatcc ttcaggtgca
     3361 gcttttcttt tttcagggtt tcgatttctt catggctggc cggctcgatg cgggcttcca
     3421 tgttcttgat gcgctggtcg aggtcgttgt gcttgtcgaa cagacgggtg aagtgggcgt
     3481 cttcggtttt caggcgggtg atcaggtcac ggtattcggg gaacattggg gtctccttgg
     3541 gggaaaactg cacgacggcg acaatcaccg ccatgacaca gctttatgtt acccagaatg
     3601 accctggctt gtcttgatcc agcgcacaca ttatgtgcac tgtgctggtg catgtgtcca
     3661 caaaagtgga cagccggtgt ggtgacaccg gccggcacgc atcaggccac gcccagatcg
     3721 cgcggcgatt cggcgctgtc cagcgcctgg cgcagggtgt cttgcagctt ttcggtgatg
     3781 gtgtagggga attgcagcgt gtcttgttcg ctgtcgtggc tgtcgtgggc gatgggggtg
     3841 gcttgctcgg tcatgatgcg ctcctgagaa ttgtgttgca ctgcacaaat cataggcgcg
     3901 cgcacggttc ttgaccagtg tctggccatc atggcgcata cagatgtcgc aatcttgtca
     3961 cactgacttg cttgctatca taaacgtcat caaaacaatt gcttgcctgc cacttggcat
     4021 gacctgtttg ggccagaaaa atggggcggc cctggcgggc gcgccccatc ttgcgctatg
     4081 gccgtaggcg acgatcaact gaccgttttg cccggtgccg gcaccggctt gcccttgttg
     4141 gccttgagcg cagcagcctg ggcggcggct tcttctgccg cagccgcttc ggcggccagt
     4201 tcttcttcgg tttttggcag caccggcgtc cattgctgtt tgccgtgctg ttcgatcacc
     4261 tggcggtagg agtgttgcag cttctggtat ttgccgttga gcgcgtccat ctgccgcacg
     4321 cgctccagga agtgatacgc gtgggccata tgggtttcgt gccagccctt gcggtgcacc
     4381 atggccagga tggcgttgac ggcgttgagc agaatctgct ggttctgtgg cagttcttcc
     4441 accgcctgca tgaacatctg cacggcgtct tccaactggc cggattgcgc cagacgcacg
     4501 gcctggttgt tgatgtccac caccgattgc acgttttcgg caatcagctg cttgcccact
     4561 tccaggcggt ccacctgttc gtaaatgcgg gtcaggcgct ccagcacatg ctggttatcg
     4621 tggttgttgc gcacgatctg ctgcaccatg ctgttgccga catcttcgcg gccctgggcg
     4681 tagcaggcct gaatcagttc catcttggcc tgctgcgaga gcttttcttc cagctccggc
     4741 atgcgcttga tggcttcgtc cagcaattcg cgaccacggt gcggctggcc ttgttttttg
     4801 aacacctggc tgtcgatgat ggtggccatc cactcggcca cggcattgtg gcggaattca
     4861 cggcgcacgg tgccggcggt ctggttggcc gattgcaggt cgcccttggc gatttgcacc
     4921 cgcgccagcg cggcgtagtc ttcgaccgcg cggtaaaccg aatatttggc cacgtcgatg
     4981 gtttttttca gtgcgcgctc ggcggtgtcg tagtcgtgat tttgcacggc gacttctgcc
     5041 agctcttttt gccggcgaat ggccagcggc gacagctgca cggcgttttc cagggtttcc
     5101 tgggcggcgg acatgtcgtt ttgtgcttcg tacagcttgg ccagccagtc gtaggcttcc
     5161 atcacccggc cgtttttcga cagcacctcg tcaaacatca ccttggcttc gtcgtactgc
     5221 ttgagctggg tcagcgattt ggccaggccc agttgtgccc acggctgcgc cttgatcgcc
     5281 atcacttctt cgtacagcga tttggccgac agataatcgc caatcttcag cgacaggctg
     5341 ccgcgcaggc gcatgaaatc cagcgcgaat tcatcgccct gcgaaatacg atcgttgcag
     5401 gagttgatcg ccaccaggag gtcgttttgc ttgagcgcgt cgtccacgca gcggaacgcc
     5461 tcgcgccggc gcatggcgcg atccaggcgc acgcgcagct gttcgccggt aaacggcttg
     5521 atcagatagc catccggctc cagctcggca gcggccagca cgcgctgcga gcggcgttcg
     5581 cccgagacga tcatgaacac gcaggattgc ttgagcagat tgcgtgtttt ggcttcttcc
     5641 agcaggtgca ggccgtcgta gccattgccc aggtcaaagt cgcacagcac gatatcaaaa
     5701 gaatagcggg ccagcttggc catggcgtcg cccggtcggg tggcgaattc caccttgttg
     5761 gcgccaaagc tggccagggt catggtcagc gcccgctgca tctcaggcac cgagtcgatg
     5821 atcaggatca gtttggacga ataagggttc tgatcgtcgt gggtcatcaa cttcggattg
     5881 gtgcggttca ccagggtttg ataagaagcc gacagttgtt gatctttcat aggctcagtt
     5941 ccgtgaatgc gtgagagtcc atttcgtcat caggtcgcga atcaagccgg agtcagcttg
     6001 gcaaaccgca tgtccagcca tttcgcgccg tgttcggcga aattgacata cagcctcaat
     6061 tcctcaccca atgcttcggc gttcaggata acaccgctac cgaatttcgg gtgagtcacc
     6121 gattggccaa tgcgccagcc gccaggcaac gcggtgtttg ccgacgcctg atggcgcggc
     6181 gccgaggcag gcgccgggcg tgtgctgcgt tcgctgcgag atgataatcc ggccgtcatc
     6241 gtctgggaga ggttttttgt caaactctcc gggatttcat ccataaagcg cgacggcagc
     6301 gggtagcggg tctggccgtg cagcatacgt tgttgcgctt gcgtcaaata caagcgtcgc
     6361 cgcgcgcggg tgatcgccac atacatcaag cgccgctctt cttcgacgcc gtgcgcgtcg
     6421 ttcagtgaat tctcgtgcgg aaacagccct tcttccaggc cggagacaaa caccgaagca
     6481 aattccagac ccttggccgc gtgcacggtc atcagctgca aggcgtcctc gccggcgcca
     6541 gcctgatgct caccggcttc cagcgaggca tgcgccagaa actcggcgat gatctgcgcc
     6601 gggtcttccg gggcaaagct caccgccgcc gaaatcagct cgtccaggtt ttccagccgg
     6661 tcttcgccat ctttatcggc ctcgtacatg gcgcgcaggc cgctggcggc gatcaaatgg
     6721 ctgaccagct gggcaaacga caagcgctcg gcgtcggcgc gcagttgttc aatcaatagc
     6781 acaaacttgc ccaggctggc ggcgctgcgc ccgccgccct ggccgcaggc ggcctgccac
     6841 aggctgacgc cgcgctcgcg cgaggcggtt tgcaggtttt ccaccgtgcg tgcgccaatg
     6901 ccgcgcgccg gcacattgat cacccgcagc aaggcattgt cgtcgtccgg gctggccacc
     6961 aggcgcaaat acgccagcgc gtgcttgatt tcctggcgct cgaaaaagcg caggccgcca
     7021 tacacccgat aaggcaggcc ggcttgaaac agcgcgtgct ccagcacccg cgactgggcg
     7081 ttcgagcgat acagcaccgc catctcggcc agcgcgccgc cgtcgcggcg cagcgcgcgc
     7141 acttcatcaa cgatgaattg cgcttcgtcg gcgtcgttcc agccgcgata caggcgaatg
     7201 ggctcgccag cgccggcgtc ggtccacagg tttttgccca gccggccgtc gttgtgctga
     7261 atcagcgcat tggcggcatc cagaatattg ccgcaggagc ggtagttttg ctccagcttg
     7321 atcggctcgc ccacctgaaa atccgccatc agccggcgca tattgcccac ctcggcgccc
     7381 cggaaggcat agatcgactg atcatcgtcg ccgacggcga acacgctggc gtgcgggccg
     7441 gcaaacaggc acagccaggc gtattgcagc ttgttggtgt cctgaaattc gtccaccaga
     7501 atatgcgaaa agcgcgcgcg gtaatgctcg cgcagcgcct cgttgctatt gagcatttca
     7561 tagctgcgca gcagcagctc ggcaaagtcc accacgcctt cgcgccggca ttgcgcgtcg
     7621 taggcggcgt acagctcggt gagcttggcg ttgaaggcgt cgtagggctt cagcgcctcg
     7681 gcgcgcaggc cttgctcttt atggttgttg ataaacgcct gcacctggcg cggcggaaat
     7741 ttctcgtccg acacttgcag cgccttcatc agccgcttga tcgccgccag ctggtcttgc
     7801 atgtccagaa tggtgaaggt ttgcggcagg ccggcgtcgc gccagtgcgc gcgcaaaaag
     7861 cggttggcca ggccgtgaaa agtgccaatc cacatgccac gcgggttcag cggcagcatg
     7921 gccgacagcc gggtgtgcat ttcgcgcgcg gctttgttgg tgaaggtcac cgccatgata
     7981 ttggccgcca tggcgcgctg gttggcaatc agccaggcaa tgcgcgtggt cagcacccgg
     8041 gttttgccgc tgccggcgcc ggccagcacc agcgccgagc ccgcgcccag cgtcactgcg
     8101 cgcaattgtt ccgggttcaa gccggccaga tgatcggaaa acatgaggct ctcaagcaag
     8161 gcaaacgcgc gattctacct gaatttggcg gcaatgaccg agtgtggctg ccagtgttct
     8221 ggcggccggc gggtggtgcg agcgcttgat tttgctgaaa atcctgccag ttttggccag
     8281 atgccgatgg cgaatgcggc gctggcggca gattctggca tgttgtaggg taagcacgcg
     8341 ggttttggct tgaattcaaa caaacgtttg gatatgctgc cgtctggcga gcccactcaa
     8401 gatggcccgc accaccctgg agaatggaga agattcatgg cgtatttcac cctcgagctg
     8461 caagacagcg tctatgtgct gaccatgatc aatggcgaca aaggcaatgt gatggacgac
     8521 gcctggctgg acgagctgac cgtccacctg gacgccatcg aagccgaccc ggcggccaac
     8581 cgcgcgctgg tgctggcgtc caatcacgaa aaagcctgga gcaatggcat tgacctggac
     8641 tacatccgca gcaagggcat ggcctatctg ttcgaccatt ttgtgccgcg cctggatggg
     8701 tttctgggcc gcatcgcctg gctgaacctg cccaccgtgg cctgcatcag cggcaatgcc
     8761 tatggtggcg gcgcgctgat tgccagcgcc tgtgattttc gcaccatgcg cgccgaccgt
     8821 ggccgcatct gctttccgga aatcgacctc aagctgggcc tgtcgccggc catggtcgag
     8881 tgcgtgaaca ccttgcccaa cgagcaggcg cgctgggaaa tggtgatgac cggccgtgcg
     8941 ctgggcggcg aagaagccgc cgcgcggcaa attgtcgatc gtgcgctgtc ggccgaggcg
     9001 ctgctgccgg aaactctggc gttggccgcc caactggcca gcaaggaccg ggtggcctac
     9061 acccggctca aacgcctgct gcgcccgcgc tgggccaagt ttgccccgca ggccaaatca
     9121 gccgcctgat tatcccggca agcgctccgg cgcgctcagg ccaaagtgct ggtaggccag
     9181 cacggtggcc atgcgcccgc gcggggtgcg ttgcagcagg ccttgctgaa tcaaatacgg
     9241 ctccagcacg tcttcgatgg tgtcggcgct ttcgccaatc gccgctgcca cattgtccag
     9301 cccgaccggg ccgccgctga atttttcgat aatcgccgac agcagtttgc ggtccatcac
     9361 gtccaggccg gcgtgatcca catccagcat cgacaacgcg gcgtcggcaa tctcgcggct
     9421 gacgcggcca tcgctgcgca cttcggcaaa gtcgcgcacc cggcgcagca ggcggttggc
     9481 aatgcgcggg gtgccgcgcg cgcggcgggc gat
//
LOCUS       DAOPYX010000098         9462 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_8534_length_9462_cov_7.854789, whole genome shotgun sequence.
ACCESSION   DAOPYX010000098 DAOPYX010000000
VERSION     DAOPYX010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 9462)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9462)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9462
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8534_length_9462_cov_7.854789"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..170
                     /locus_tag="PKN55_06610"
     CDS             <1..170
                     /locus_tag="PKN55_06610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009118195.1"
                     /note="Sms; stabilizes the strand-invasion intermediate
                     during the DNA repair; involved in recombination of donor
                     DNA and plays an important role in DNA damage repair after
                     exposure to mutagenic agents; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HNX70947.1"
                     /translation="RPVAHGQERLKEAAKLGFCRAIVPQANAPRQGIDGLELIPVERL
                     EQAVDYCRSSA"
     gene            complement(149..853)
                     /locus_tag="PKN55_06615"
     CDS             complement(149..853)
                     /locus_tag="PKN55_06615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018747804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNX70948.1"
                     /translation="MADVMVLQGIRQRYGGGDSPLVEVLHGIDLKLPEGGFSALIGPS
                     GSGKSTLLNIIGLLESPTEGQLWLAGEQASGQNDRVRTALRNRYLGFIFQFHHLLPAF
                     SAEENVLMPVLAGVGRISAADRERARTLLARVGLAEHAHKRPVQLSGGQQQRVAIVRA
                     LINRPRLVLADEPTGNLDSQTASEVFALLRELNREEGMSFLIVTHDPRLAQSCDQQFY
                     LQDGRLLPPAQADERQ"
     gene            complement(846..2063)
                     /locus_tag="PKN55_06620"
     CDS             complement(846..2063)
                     /locus_tag="PKN55_06620"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNX70949.1"
                     /translation="MSFIWTVAWRLLREGRFQTALILGGVTIGVGVIVYITAIVNGLQ
                     ANIIERTLSSQAHVVLRPADRLNQAALSAPDGVTPLADVRKRTQRENTIANWTPLLHE
                     IRAHPRVTASAVMASGPGVAQQGGVRKSVSLMGIELDDYQTVVRLDNKLRSGQLQLAA
                     GEALIGVELAADLGLSPGSRLRLQSASGEQISVRVAATLDFGLKDLNRRWVLMPLRDA
                     QNLLGYRLDITEIYVRTDQLFEAETLAGQLSASTGLTADSWQASNGQLVTALKSQRAS
                     STMIRVFVTFAVALGIASVLVVSVVQRQREIGILRAMGTPAWRILGVFLLQGGIVGLV
                     GALFGTGLGAGLALGFTQIARTEDGGPLFPVVLDSELFISTALIAFVVGLLSALMPAL
                     RAARLDPVEAIRG"
     gene            complement(2060..3100)
                     /locus_tag="PKN55_06625"
     CDS             complement(2060..3100)
                     /locus_tag="PKN55_06625"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNX70950.1"
                     /translation="MKLQGKWLWRGALTLLALALAWLWLRPTPVTTLTLAAQPVQRSV
                     VATGRVEAIRESVLGSTVTARVVATPVKQGAHVAAGATLLLLESDELLALRAQAEAQL
                     RDADSQLSEARRQWQRQQALFAQGFISQAALDAARRTLDAAEQRQAQTRGALAQSQAK
                     LEQMTVRAPADGVLLTRAVEAGDVVTAGKTLLRFASDGPRRVLLDLDERDLGKVALGQ
                     TAAVLADAFPQLRAPAVVSRINASVDNARGTVEIELALTAAAEHLRSGMTVSAEILTR
                     PAQATLLLPATAVREGRAALLEDGRVRWVKISTDERIAGQLPVTAGLSAGQQVIVPAP
                     LLDDGARVKAQP"
     gene            3668..6307
                     /locus_tag="PKN55_06630"
     CDS             3668..6307
                     /locus_tag="PKN55_06630"
                     /inference="COORDINATES: protein motif:HMM:NF012573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HNX70951.1"
                     /translation="MATSTSTASITLAATATTYALSVSGDQAVYGNALSNSLSGNAFD
                     NFLSGNAGNDTLLGGLGNDTLLGGDGADRLDGGIGADSMAGGDGNDIYYVDDVNDIVT
                     ELAGQGMDTVWASVSYTLGANVEALGLTGTANLTGTGNELANSISGNAGDNLIDGGAG
                     NDTLLGGAGNDTLLGGEGNDLLNGGVGADVLMGGEGSDRLEGGLDADTMQGGAGNDVY
                     VVDNIGDVVIEDVNGGTDTVWASMDYTLGDNVESLGLTGTANLTGIGNALANSISGNT
                     GNNLLDGGAGNDILLGGAGDDTLLGGDGNDRLDGGVGADVLVGGAGNDLYVVDDLGDV
                     IVEDANGGIDSVSASIDYTLGDTLEALTLTGTADLTGTGNALANSITGNSGNNRPDGG
                     AGNDTLVGGLGQDTLIGGLGNDTFVVDDIGDVVVEEAGQGTDTVQSSISYTLADTLEN
                     LSLIGSANLTGTGNALANTLIGNAGNNVLDGGAGNDVLIGGLGADTLIGGAGNDGYQV
                     DDIGDVVVENPDEGVDAVTIIATLNYTLGDNVENARVNSSGSMLTGNAMANQIVSLGV
                     SSIIYGMDGNDTISGGASSSLYGDNGDDSLTLTGTGSFAYGGTGNDTLDARTTTGNWL
                     VGGAGDDVYVVDSTNQYILERANEGVDTVKSSVTFSLVAGARTGGGSVSDSLENLTLT
                     GTNAIDGTGNALGNVLTGNAAANVLLGNAGNDTLIGGGGNDTLNGGTGNDTLRATADG
                     ATGAALAGAVTLIGGAGNDVFAMSKGYVGNHASGVNTLTIQDFVHGEDKISLTLAKTM
                     TQPGALQQLTVGTSDTLDSLLARATTGGGPTAPKVSSFVFAGNTYLVLDQNAINGFAA
                     TDLAIKVTGTPALSFSDLAFAVV"
     gene            complement(6374..6844)
                     /locus_tag="PKN55_06635"
     CDS             complement(6374..6844)
                     /locus_tag="PKN55_06635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018747359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HNX70952.1"
                     /translation="MTEAVAAWRRKAVDLLARREYSRAELARKLAGREADIDRAALDA
                     VLDELAACGWQSDARFAQMWIQAHAARHGAQRLRQDLRQRGVATADIDAALAEHQAAE
                     PEQDELSRARAAWARKFAALPTNREDYARQYRFLASRGFASGIVSKVLSGRDRD"
     gene            complement(7108..8190)
                     /gene="recA"
                     /locus_tag="PKN55_06640"
     CDS             complement(7108..8190)
                     /gene="recA"
                     /locus_tag="PKN55_06640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012696278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HNX70953.1"
                     /translation="MADNDKAKALAAALAQIEKQFGKGSIMKMNETQVNENLQVISTG
                     SLGLDLALGVGGLPRGRVVEIFGPESSGKTTLCLQVVAEAQKLGGTCAFIDAENALDP
                     QYARKLGVDVDDLLISQPDTGEQALEICDMLVRSGGVDVIVIDSVAALVPKAEIEGEM
                     GDSHVGLQARLMSQALRKLTGNIKRTNTLVIFINQIRMKIGVMFGSPETTTGGNALKF
                     YASVRLDIRRIGGIKKGDEVIGNETRVKVVKNKVSPPFRQADFDILYGEGISREGEVI
                     ELGVTHKFIEKSGAWYSYNGQKIGQGKDNVREYLKDNPGLAHEIENKVRNAVGVNGLP
                     EFEPSAEERKEAALTEDSKLDDGSLL"
     gene            8447..>9462
                     /gene="hrcA"
                     /locus_tag="PKN55_06645"
     CDS             8447..>9462
                     /gene="hrcA"
                     /locus_tag="PKN55_06645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_043595811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HNX70954.1"
                     /translation="MLNDRAQRLLKTLVERYIADGQPVGSRTLSMHSGLDLSSASIRN
                     VLADLENMGLVISPHTSAGRVPTARGYRVFVDNLLTMQPLEQAARQQLESELHPDSPQ
                     RIVSAASQLLAELTHFAGVVATPERASVAFRQIEFLRLSEKRVLLIFVTADGDVQNHL
                     LLTERDYTASELIEAANFLNHHGAGRSLEAVARELEGELRNLQSHIASLMGAAIVAGQ
                     NTLGPRQSVMVTGEKNLLNSDDLSANLINLRQLFDLFERKTELLRLLNLSREAHGVHI
                     FIGEESGLNTLDGCSVITAPYQINGQVVGTLGVVGPTRMAYERVIPIVDITARLVSSA
                     LSYPV"
BASE COUNT         1686 a         2923 c         3002 g         1851 t
ORIGIN      
        1 tgcgcccggt ggcgcacggc caggagcggc tgaaagaagc cgccaagctg ggcttttgcc
       61 gcgccatcgt gccgcaggcc aatgcgccgc gccagggtat tgatgggctg gagctgattc
      121 cggtggagcg gctggagcag gcggtggatt attgccgctc gtcggcctga gccggcggca
      181 gcaagcgccc gtcctgcaaa taaaactgct gatcgcagct ttgtgccaga cgtgggtcgt
      241 gggtgacgat caaaaaactc atgccttctt cgcggttcag ctcgcgcagc agggcaaata
      301 cctcgctggc ggtctggctg tccagattgc cggtgggctc gtcggccagc accagccggg
      361 ggcggttgat cagcgcgcgc acaatcgcca cccgctgctg ctgcccgccg gacaattgca
      421 ccggccgctt gtgcgcgtgc tcggccaggc ccacccgcgc cagcaaggtg cgggcgcgct
      481 cgcggtcggc ggcgctgatg cgcccgacgc cggccagcac gggcatcagc acgttttctt
      541 cggcgctgaa cgctggcaat aagtggtgaa actgaaaaat aaaacccaga taccgattgc
      601 gcagcgccgt gcgcacgcgg tcgttttgcc cgctggcctg ctcgccggcc agccacagct
      661 ggccttcggt gggcgattcc agcaggccaa taatattcag cagggtggat ttgcccgagc
      721 cagacggccc gatcagcgcg gaaaacccgc cttccggcag cttaagatca atgccgtgca
      781 gcacttccac cagcggcgaa tcgccgccgc cgtagcgctg gcgaatgcct tgcaacacca
      841 tcacatcagc cacgaatcgc ctccaccggg tccagccgcg cggcgcgcaa cgccggcatc
      901 agcgccgaca ataagcccac cacaaaggca atcagcgccg tgctgatgaa cagctcgctg
      961 tccagcacca ccggaaacag cggcccaccg tcttcggtgc gggcaatctg ggtaaagccc
     1021 agcgccagcc cggcgcccag cccggtgcca aatagcgcgc ccaccaggcc gacaatgccg
     1081 ccttgcaata aaaacacccc caaaatccgc caggccgggg tgcccatggc gcgcagaatg
     1141 ccgatttcgc gctgacgctg caccaccgac accaccagca cgctggcgat gcccagcgcc
     1201 acggcgaagg tgacaaacac gcgaatcatg gtgctggagg cgcgctggct tttcagcgcg
     1261 gtgaccagct ggccattgct ggcctgccag ctgtcggcgg tcagcccggt gctggcggat
     1321 aactgcccgg ccagggtttc cgcctcaaac agctggtcgg tgcgcacata gatttcggtg
     1381 atgtccagcc ggtagcccag caggttttgc gcgtcgcgca gcggcatcag cacccagcgg
     1441 cggttgaggt ctttcaggcc aaaatccagc gtggcggcca cccgcaccga gatttgctcg
     1501 ccgctggccg attgcaggcg cagccggctg cccggactca ggcccaggtc ggcggccagc
     1561 tcgacgccaa tcagcgcctc gccggcggcc agctgcaatt ggccgctgcg cagtttattg
     1621 tccagccgca ccacggtttg gtaatcatcc agctcaatgc ccatcagcga caccgatttg
     1681 cgcacgccgc cctgctgcgc cacgccgggg ccgctggcca tcaccgccga ggccgtcacc
     1741 cgtggatggg cgcggatttc gtgcagcagc ggcgtccagt tggcgatggt gttttcccgt
     1801 tgggtgcgct tgcgcacgtc ggccagcggg gtgacgccgt ctggcgcgct cagcgccgct
     1861 tggttgagcc ggtcggccgg gcgcagcacc acatgcgcct ggctggacag ggtgcgctcg
     1921 atgatattgg cttgcaggcc gttgacaatg gcggtgatgt agacaatcac gccgacgccg
     1981 atggtcaccc cgcccaaaat cagcgcggtc tgaaaccgcc cttcgcgcaa taagcgccag
     2041 gccaccgtcc agataaaact catggctgcg ccttcacccg cgcgccgtca tccagcagcg
     2101 gcgccggcac aatcacctgc tggccggcgc tcaggccggc cgtcaccggc aactggccgg
     2161 caatccgctc gtcggtgctg atcttcaccc agcgcacgcg gccgtcttcc agcagggcgg
     2221 cgcggccttc gcgcacggcg gtggccggca gcagcagggt ggcttgcgcc gggcgggtga
     2281 ggatttccgc cgagacggtc atgccgctgc gcaagtgctc ggcggcggcg gtcagcgcca
     2341 gttcgatttc caccgtgccg cgcgcattgt ccacgctggc gttgatgcgg ctgaccactg
     2401 ctggcgcgcg cagttgggga aaggcgtcgg ccagcacggc ggcggtttgt cccagcgcca
     2461 ccttgcccag atcgcgttcg tccagatcca gcagcacccg gcgcgggccg tcgctggcaa
     2521 agcgcagcag ggttttgccg gcggtgacca catcgcccgc ctcaacggcg cgggtcagca
     2581 gcacgccgtc agctggcgca cgcacggtca tttgttccag tttggcctgg ctttgcgcca
     2641 gcgcaccgcg cgtttgcgcc tgacgctgct cggcggcgtc cagcgtgcgc cgggcggcat
     2701 ccagcgccgc ctggctgata aagccttggg caaacagcgc ctgctggcgt tgccactggc
     2761 ggcgggcctc gcttagctgg ctgtcggcgt cgcgcagctg cgcctcggct tgcgcgcgca
     2821 gggccagcag ttcgtcagat tccagcagca gcagggtggc gccagcggcg acatgcgcgc
     2881 cttgtttgac cggggtggcc accacccgcg cggtgacggt gctgcccagc acgctttcgc
     2941 ggattgcctc cacccgaccg gtggccacca ccgagcgttg caccggctgc gccgccaggg
     3001 tgagcgtggt gacgggcgtg gggcgcagcc acagccaggc cagcgcaagc gccagcaatg
     3061 tcagtgcgcc gcgccacagc catttgccct gcaatttcat gggagtcttt ctggatcaag
     3121 cctgcgggaa ttatgcccga tgtggctgag agaaaacatg atgttcgtgg gtggcttgtt
     3181 ggcgggggcg gtgtggtttg tgggcgacat accagatttt tttcttgcgt tccagtgctt
     3241 ggatcaaggt tgtgacattg catcgttacg tataattacg tatagtagat atacgtatcg
     3301 tacttctacg tagtgctgtg gcgaaacact ttgctcagtc acgcgggaag tttgcggcgc
     3361 aacgctgccg ccatgctgtc cgtcaccctt ggggagggaa cggagcccag ccaggtctgc
     3421 cttttctcaa aaggcggcag cctgtgaaca gcagctgaac ttccccagtg tgacagtaca
     3481 gcgcatgttc ctggcttttg ccctgatcct ttcatgatct tccgatcatg cctgccgtca
     3541 ccacttcctt gtctctgtcg ccatcctggg tcatgcccac cggggcaagg tcaatgtggc
     3601 gcccgtgcca ttcaacgcgg cgtgcacgat acaaccatca ctaaagcaga caaaggattc
     3661 acgcatcatg gccacttcca ccagtaccgc gtccattacc cttgcggcca ctgcgactac
     3721 ctacgcactg agtgtcagtg gtgatcaagc cgtgtatggc aatgcactct ccaacagtct
     3781 ctctggcaat gcgtttgaca atttcctgtc tggcaatgcc ggtaacgaca ctttgctggg
     3841 tggccttggc aacgatacct tgctgggcgg ggatggcgct gatcgtctgg atggtggcat
     3901 cggggcggac tccatggcgg gaggggatgg caacgacatt tattatgtgg atgatgtcaa
     3961 tgatattgtc accgagctgg ccggtcaggg catggatacg gtatgggcca gtgtcagcta
     4021 taccctgggg gccaatgtcg aggctttggg cctgaccggt acggccaacc tgaccggtac
     4081 tggcaatgaa ttggccaatt ccatcagcgg caacgcgggt gacaacctga ttgatggcgg
     4141 cgccggtaac gatactctgc tggggggcgc aggaaatgac accctgctgg gcggcgaggg
     4201 caatgatttg ctcaacggcg gggttggggc agatgtcttg atggggggcg aaggcagcga
     4261 ccgtctggag ggcggcctgg atgctgacac catgcagggt ggcgcgggca atgatgtcta
     4321 cgtggtagac aatatcggcg atgtggtcat tgaagacgtg aacggcggca ctgacacggt
     4381 gtgggccagc atggattaca ccttgggcga taatgtcgag tctctgggct tgacgggtac
     4441 cgccaatctg accggtatcg gcaatgcgct ggccaattcg atcagtggca acaccggtaa
     4501 caatttactg gatggtgggg ccggtaacga tatcctgctg ggcggtgctg gcgatgacac
     4561 cttgctgggt ggcgacggta acgatcggct ggatggcggc gttggtgccg atgtcctggt
     4621 gggtggggct ggcaacgatc tgtatgtggt tgatgatctg ggtgatgtga tcgtcgagga
     4681 tgccaatggg ggtattgatt cggtctccgc cagcattgat tacaccctgg gtgataccct
     4741 ggaggcgctg accctgaccg ggacggcgga tctgaccggc accggcaatg ccctggccaa
     4801 ttcgatcacc ggcaacagcg gcaacaatcg tcctgatggc ggggccggca acgatacact
     4861 ggtgggcggt ctgggtcagg ataccttgat tggtggcctg ggtaatgaca catttgtggt
     4921 ggatgacatc ggtgatgtgg tagtggaaga agcaggccaa ggcactgaca ccgtgcagtc
     4981 cagcatcagc tacaccctgg ccgataccct ggagaatctc agcctgatcg gctcggccaa
     5041 tctgaccggc acgggcaatg cattggcgaa tacgctgatc ggcaatgccg gcaacaatgt
     5101 gctggatggt ggcgctggca atgacgtcct gattggcggc ctgggcgcgg atacgctgat
     5161 cggcggtgcg ggtaacgatg gctatcaggt ggacgatatt ggcgatgtgg tggtggaaaa
     5221 ccccgacgaa ggggtggacg ctgtcaccat cattgccacg ctgaattaca ccctgggtga
     5281 caatgtcgaa aatgcccgtg tcaacagcag cggttcgatg ctgaccggca atgccatggc
     5341 taaccagatt gtgtcgctgg gcgtctccag cattatctat ggcatggacg gcaatgacac
     5401 catcagtggc ggcgccagct ccagcctgta cggtgacaat ggcgacgata gcctcaccct
     5461 gactggcacc ggttcgtttg cctatggtgg caccggcaac gacaccctgg atgcccgtac
     5521 gaccaccggc aactggctgg tgggtggggc gggcgatgat gtctatgtgg tggacagcac
     5581 caatcagtac atcctggagc gtgccaacga aggcgtggac accgtcaaga gcagcgtgac
     5641 gttctccctg gtggcagggg cgcgcacggg gggcggctcg gtgtccgaca gcctggaaaa
     5701 cctcaccctg accggcacca atgccattga cggcaccggt aatgcactgg gcaatgtgct
     5761 gactggcaat gccgccgcca acgtgttgct gggcaatgcc ggcaatgaca ccctgattgg
     5821 tggtggcggc aacgataccc tcaatggcgg caccggtaat gacaccttgc gcgccactgc
     5881 cgatggggcc actggcgcgg cgctggcggg agccgtcacc ctgatcggtg gcgctggtaa
     5941 tgatgtgttt gccatgagca agggctatgt cggcaaccat gccagcggcg tcaataccct
     6001 gaccattcag gattttgtgc acggcgaaga caagatcagc ctgacgctgg ccaagaccat
     6061 gacccagcct ggcgccttgc agcaactgac cgtcggcacc agcgatacgc tggatagcct
     6121 gctggcgcgc gccaccacgg gcggtggccc gactgcgccc aaggtcagca gctttgtgtt
     6181 tgccggcaat acctatctgg tgctggatca aaatgccatc aatggttttg ctgccaccga
     6241 tctggccatc aaagtcaccg gcacgccggc cctgagtttc agtgatctgg cttttgccgt
     6301 ggtgtaacat cgctgtctct ggcttggccg caaaaccgcc cctgaccgct gtacggcagg
     6361 ggcggttttg cgttcaatcg cggtcacggc cgctcagcac cttgctgact atcccgctgg
     6421 caaagccgcg tgacgccaaa aagcggtatt gtcgggcgta gtcctcgcgg ttggtcggca
     6481 gtgcggcaaa tttgcgtgcc caggcggcgc gagcgcggct gagctcgtcc tgctctggct
     6541 cagcggcctg gtgttcggcc agcgcggcgt cgatatcagc cgtcgccacc ccgcgctggc
     6601 gcaaatcctg gcgcagccgc tgcgcgccgt ggcgggcggc gtgcgcctga atccacatct
     6661 gggcaaagcg cgcgtcggat tgccagccgc aggcggccag ctcgtccagc acggcgtcca
     6721 gcgccgcgcg gtcgatgtcc gcctcgcggc cagccagctt gcgcgccagc tcggcgcggc
     6781 tgtattcacg gcgcgccagc aaatccaccg ccttgcgccg ccacgcggca acggcctctg
     6841 tcatgggctg cgccctccag aaaacacagc cgggctggca gatgccagcc cggcttgaga
     6901 tgccagtcta acgacttggc gccgctcaca aaaccctcct ggcgttgtta cccgaccttg
     6961 ccgtacttgt tgtactgtct gcggtcgtgc gcctagccag gagcgctgcg ctgggttttg
     7021 tgagcgacgc taagcgccgc tcacaaaacc caacaaggca atgttggcca agcgcgcaac
     7081 accaccaaac gggttttggt caaccactta caacagactg ccgtcgtcca gcttgctgtc
     7141 ttcggtcaag gcggcttcct tgcgctcttc tgcgctgggt tcgaactccg gcaggccatt
     7201 cacgcccact gcgttgcgca ccttgttctc gatttcgtgc gccaagccgg ggttgtcttt
     7261 caggtattcg cggacattgt ccttgccctg gccaattttc tggccattgt agctgtacca
     7321 ggcgccggat ttttcgatga acttgtgcgt gacgcccagt tcgatcactt cgccttcgcg
     7381 gctgatgcct tcaccgtaca gaatatcgaa atcagcctga cggaacggcg ggctgacctt
     7441 gttcttgacc actttcactc gggtttcgtt gccgatgact tcgtcgccct tcttgatgcc
     7501 gccaatgcgg cggatgtcca gacgcaccga ggcgtagaac ttcagcgcat tgccgccggt
     7561 ggtggtttcc gggctgccaa acatcacgcc aatcttcatg cggatctggt tgatgaaaat
     7621 caccagggtg ttggtgcgct tgatattgcc ggtcagcttg cgcagcgcct ggctcatcag
     7681 ccgggcttgc aggccaacat ggctgtcgcc catttcgcct tcgatttccg ccttgggcac
     7741 cagcgccgcc accgagtcga tgacaatcac gtccaccccg cccgagcgca ccagcatgtc
     7801 gcagatttcc agcgcctgtt cgccagtgtc cggctgcgag atcagcaggt catccacatc
     7861 cacgcccagc ttgcgggcgt attgcggatc cagcgcgttt tccgcgtcga taaaggcgca
     7921 ggtgccgccc agcttttgcg cctcggccac cacttgcaga cacagcgtgg ttttgcccga
     7981 ggattccggg ccaaagattt ccaccacccg accacgcggc agcccgccca cgcccagcgc
     8041 cagatccagc cccagcgagc cggtggagat cacttgcagg ttttcattga cctgcgtctc
     8101 gttcattttc atgatcgagc ccttgccaaa ctgcttttcg atctgcgcaa gagcggcggc
     8161 cagcgctttg gccttgtcgt tgtctgccat ggtgtctcca gggaataaga taaaatgccc
     8221 gcatcgcggc aatgccggca ttatggcaca caattggcgt gacagaaacg cgaattgtgt
     8281 gacggggtga taaatctgtc gcgtagatgg tccattcata tgaatgctga ttcatatgaa
     8341 tggaccatcg ctgacacatc tcagcgcccc acgcttgaaa tttatccggc tgcgccgatg
     8401 ttcaactcac ctctgttgtt ttctgctgtt tcaggaatgt cctcccatgc tcaacgaccg
     8461 cgcccaacgc ctgctgaaaa ccctggtgga acgctatatt gccgatggcc agccggtggg
     8521 ctcgcgcacc ttgtcgatgc attccgggct ggatctttcc agcgcgtcga ttcgcaatgt
     8581 gctggccgat ctggaaaaca tgggcttggt gatcagcccg cacacctcgg ccggccgggt
     8641 gccgacggcg cgcgggtatc gggtgtttgt ggataatctg ctgaccatgc agccgctgga
     8701 gcaggcggcg cgccagcagc tggaaagcga gctgcacccg gacagcccgc agcgcattgt
     8761 cagcgccgct tcgcaattgc tggccgagct cacccatttt gccggggtgg tggccacgcc
     8821 ggagcgcgcc agcgtggcgt ttcggcagat tgagtttttg cgcttgtcag aaaagcgcgt
     8881 gctgctgatt tttgtcaccg ccgatggcga tgtgcaaaac catttgctgc tgaccgagcg
     8941 cgactacacc gccagcgagc tgatcgaggc ggccaatttt ctcaatcacc acggcgccgg
     9001 gcgcagcctg gaggcggtgg cgcgcgagtt ggaaggcgag ctgcgcaatc tgcaaagcca
     9061 tatcgccagc ctgatgggcg cggcgattgt cgccgggcag aacaccctgg ggccgcgtca
     9121 gtcggtgatg gtgactggcg aaaaaaacct gctcaatagc gatgacttgt cggccaatct
     9181 gattaatctg cgccagttgt ttgatttgtt cgagcgcaaa accgagctgt tgcgcctgct
     9241 caacctcagc cgcgaggcgc atggcgtgca tatctttatt ggtgaggaat ccggcctgaa
     9301 caccctggac ggctgttcgg tgatcaccgc gccgtatcag atcaacggcc aggtggtggg
     9361 cacactgggg gtggtggggc ccacgcgcat ggcgtatgag cgggtgattc cgattgtgga
     9421 catcaccgcg cggctggtgt cgtcggcgct gtcttatccg gt
//
LOCUS       DAOPYX010000099         9421 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_8586_length_9421_cov_7.125988, whole genome shotgun sequence.
ACCESSION   DAOPYX010000099 DAOPYX010000000
VERSION     DAOPYX010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 9421)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9421)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9421
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8586_length_9421_cov_7.125988"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..969
                     /gene="thrC"
                     /locus_tag="PKN55_06650"
     CDS             <1..969
                     /gene="thrC"
                     /locus_tag="PKN55_06650"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012696072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HNX70955.1"
                     /translation="AAEYAMRGKQGVRVFMLSPHEKMSPFQTAQMFSLQDPNIFNLAV
                     RGVFDDCQDIVKAVSNDHAFKAKYKIGTVNSINWARVVAQVVYYFKGYFAATQNNDQQ
                     VSFSVPSGNFGNVCAGHIARMMGLPIRKLIVATNENDVLDEFFRTGRYKVRGSANTWQ
                     TSSPSMDISKASNFERFIFDLVGRDAEQVRALWQAVDSTGEFDLSASPLFAKISAEYG
                     FASGKSTHADRLATIRHTFEQYGVIIDTHTADGLKVGLEQREAGVPLICLETALPAKF
                     EATIREALDRDPPRPAGLENIEALPQRFEVMDADAEQIKAFIAAHV"
     gene            complement(1190..2131)
                     /locus_tag="PKN55_06655"
     CDS             complement(1190..2131)
                     /locus_tag="PKN55_06655"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HNX70956.1"
                     /translation="MRKSTILLSSYCLLSGVAFAGAFDPLPMPTQTVSGRQVIINVPQ
                     TRLFVLENGKLTHVFPIAVGKSLTKTPLGEYKVTDIRRNPTWFVPASIQAEMRQSGKN
                     VETAVPPGPGNPLGPVFIRFGDPALGLGMHGTNVPSSVPGFRSHGCVRLRSPDALKLA
                     ALMEKGVPVTVTYQPVLLNNDENGNLWVTAFADHYKQAAAPAKLSAQVKDLASRWSSE
                     TGRPLDEKRLLTAVQAREGKPVCVTCEQSAPAHNPQRPVSPKPDTAPAETAPAESTPS
                     AAPVEQAQPAAPADGGAAARSGNAQTRAGVLPVRNGG"
     gene            2307..3179
                     /locus_tag="PKN55_06660"
     CDS             2307..3179
                     /locus_tag="PKN55_06660"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HNX70957.1"
                     /translation="MLIPPSLRRAAIELYDAYTHTPLPRREFLRRLGVLAGGTVATSS
                     LLALLENHYAHADTVPVDDARLQHADIRFAQLDGQPLHGYLSQPASGGPRPGVVLIHE
                     NRGLNPHIRDVARRLALAGFTVLAPDYLSTQGGTPSDEDRARQAFAEMDPVLARRISL
                     AALAALRERGATKVGALGFCWGGGQVGQLAIHAADLQAAVVYYGPPPASDEVAAIRAP
                     LLLHYAGLDIRINAMLPAFETALRAAGVRHQLYRYANVDHAFNNDTNPARFNAEAAAL
                     AWSRSMAFLHAQLG"
     gene            3281..4840
                     /gene="rlmD"
                     /locus_tag="PKN55_06665"
     CDS             3281..4840
                     /gene="rlmD"
                     /locus_tag="PKN55_06665"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HNX70958.1"
                     /translation="MTHPQPIARIESLDFEGRGVAHVDGKTLFIDGGLPYETVRYSSY
                     RKKPSYENADAVQVLNASFMRTTPRCPHFGVCGGCSMQHVEFSAQVANKQRVLEDNLA
                     HIGRVTPEVILPPIYGPTWGYRHRARLSARHVPKKGGVLVGFHEKRSTYIADMRECHI
                     LPERISALLMPLRELIGQWSIVARMPQVELAVGDAVDVLVFRIMDPLTAADEALLRQF
                     IDAHATPQRPLQAWLQPKGPETCYPFYPLDAPTLTYSLPDFRVVMPYRPTEFTQVNPG
                     INRVMVARAMRLLDAQPGERIADMFCGIGNFTLPIARSGATVLGMEGSQSLVERAIDN
                     ARHNGLEDQVSYRVANLFEVTPESFAALGQFDKMLIDPPRDGAIALCKALDEDTAPAR
                     LVYVSCNPATLARDAGVLVNTKGYTLRAAGIINMFPHTGHVESIALFEKTGPCVSRAE
                     LEAQEAAEAAAREAAKAAAKVARAAKEAAEAEARAARAADKEARRQAWAAKREAEGWH
                     EASDDDTQGKE"
     gene            complement(5060..5857)
                     /gene="map"
                     /locus_tag="PKN55_06670"
     CDS             complement(5060..5857)
                     /gene="map"
                     /locus_tag="PKN55_06670"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008953621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HNX70959.1"
                     /translation="MPIIIKNAEDIAKMRVAGRLASEVLDYITPYVQPGVTTEELDRL
                     CHDYMVNQQGCIPAPLNYAPEGHAPYPKSICTSVNQVICHGVPNDKALKKGDIVNLDI
                     TVIKDGYHGDTSRTFLVGEATPQAKRLVRVTHECLWIGIDQVKPGAHLGDIGAAIQKY
                     AEACGYSVVQEFCGHGIGRGFHEEPQVLHYGRAGTGVELQPGMIFTIEPMINAGKRHL
                     KILNDGWTVVTKDRSLSAQWEHTLLVTETGCEVLTLSEGTPARPGRE"
     gene            complement(5976..7061)
                     /locus_tag="PKN55_06675"
     CDS             complement(5976..7061)
                     /locus_tag="PKN55_06675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014086681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HNX70960.1"
                     /translation="MNRKMLVVALSAACASPLALADVTIYGTLNNSLESVKATGAANS
                     ANDVKSTSRVSSNTSKIGFKGNEDLGNGLKAIWQVEQEVSIDDGGTRKGNWATRNTFV
                     GLDGRFGQVMLGNYDSAYKMVKYLIPLEDGVADFTGANGIIGRGQQRMKNSVQYRSPN
                     FSGFSFGASYGTDETRAVLANSERTNAQAWALAAQYNADGLLLGGAFERRDDTGAVAG
                     ASKTTDHQDFWKAVARYKFADTELGLGYEYENQDRIVGADQKQSAWSVSATQKFGNFG
                     LGLAYVSLGKRKGAGSGSISDADYKANQWSLSGTYDLSKRTQAYAFYTRIDNKNAAKI
                     NFDSNGITGVAAGSNPTAFGIGLSHKF"
     gene            complement(7419..7691)
                     /locus_tag="PKN55_06680"
     CDS             complement(7419..7691)
                     /locus_tag="PKN55_06680"
                     /inference="COORDINATES: protein motif:HMM:NF016226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen fixation protein NifZ"
                     /protein_id="HNX70961.1"
                     /translation="MLPPLPVIPYEVGEMVYCAEDLFNDGGMPGADEDAILAPAGARG
                     VVVQSGYAEEDESQMIYLVRFEDASGELGPPIGCLPEELTQQAPAA"
     gene            complement(7696..8022)
                     /locus_tag="PKN55_06685"
     CDS             complement(7696..8022)
                     /locus_tag="PKN55_06685"
                     /inference="COORDINATES: protein motif:HMM:NF013426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HNX70962.1"
                     /translation="MPKPKKHVLVCVQGRPPGHPRGSCQARGCNDVFQAFSNEFQQRN
                     LWANFLLTNTGCLGPCHLGPTVIVYPEGVMYTGVKPEDVGVIIDEHLMFDQPVERLLA
                     PPDVWS"
     gene            complement(8213..9283)
                     /locus_tag="PKN55_06690"
     CDS             complement(8213..9283)
                     /locus_tag="PKN55_06690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008953622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HNX70963.1"
                     /translation="MNKKLLAAALSAAFVAPAAFADVTTYGVINSSVEFAKATGATNS
                     ALDVKSVNRVTANNSRIGFKGWEDLGNGLKAIWQVEQNVDIDTGNSSTSWANRNSFIG
                     LQGDFGRVLLGKHDSAYKTMTIGLGTNILADTSADTCTSQAIFCRGDQRVSNTVQYYS
                     PVVNGFSAGVSYGADETRATVGGNRANAYSLSFGGKYVNGGLAVGAGYEHRNDSVATA
                     TGLDTDYFKLVGAYTFASATTLAAGYEHSEVDQFAKSAKKQDGFTLAVKQQLGAWGVG
                     ASYTWLGKEKNGAEGDGKAKQWVLAATYDLSKRTQVQAYATKLSNNKSANRNFGINPL
                     NGTLSAAGADPQAIGVGIRHAF"
BASE COUNT         1794 a         2899 c         2936 g         1792 t
ORIGIN      
        1 gccgccgaat acgccatgcg cggcaagcag ggcgtgcgcg tgttcatgct ctccccgcac
       61 gaaaaaatga gcccgttcca gaccgcgcag atgttcagcc tgcaagaccc gaacatcttc
      121 aacctggcgg tgcgcggcgt gtttgacgac tgtcaggaca tcgtcaaggc cgtgtccaac
      181 gaccacgcct tcaaggccaa gtacaaaatc ggcacggtga actccatcaa ctgggcgcgt
      241 gtggtggccc aggtggtgta ctacttcaag ggctattttg ccgccacgca aaacaatgac
      301 cagcaagtca gcttcagcgt gccgtccggc aactttggca atgtctgcgc cggccacatc
      361 gcccgcatga tgggcctgcc gattcgcaag ctgattgtcg ccaccaacga aaacgacgtg
      421 ctcgacgagt ttttccgcac cggccgctac aaggtgcgcg gctcggccaa cacctggcaa
      481 acctccagcc cgtcgatgga catctccaag gcctccaact tcgagcgctt tattttcgat
      541 ctggtcggcc gcgacgccga acaagtgcgc gcgctgtggc aagcggtgga cagcaccggc
      601 gagtttgacc tctccgccag cccgctgttc gccaagatca gcgccgaata cggctttgcc
      661 tccggcaaga gcacccacgc cgaccgcctg gccaccatcc gccacacctt cgagcaatac
      721 ggcgtgatca tcgacaccca caccgccgac ggcctgaaag tgggcctgga acagcgcgag
      781 gccggcgtgc cgctgatctg cctggaaacc gccctgccgg ccaagttcga agccaccatc
      841 cgcgaagcgc tggaccgcga cccgccgcgc ccggcggggt tggaaaatat cgaagcgctg
      901 ccgcagcgct ttgaggtgat ggatgccgat gctgagcaaa tcaaggcgtt tattgccgcg
      961 catgtgtaag acctgagcca gcgtcaggcc accgcctggc cgcgtggtca aaatacgccc
     1021 cggtctgatt ggctcagacc ggggcgtatt gtttgcgggt cagctcgcct cattggccgg
     1081 cagaagcggc aacaatcagc cggtccccag gacggcatct tgcccgacgc gccagacggc
     1141 agatttggcc aaaaaatcag ccggcagcgg gctgagcatg cgcgaaacct cagccgccat
     1201 tgcggacagg cagcacgcct gcgcgggttt gcgcattgcc cgaacgggcg gcagctccgc
     1261 catcagctgg cgcggcgggc tgcgcctgct cgacgggcgc ggcggacggc gtgctttcag
     1321 ccggggcggt ttcagccggc gcggtgtctg gtttgggtga taccgggcgc tgcgggttgt
     1381 gcgccggggc agactgctca caggtgacgc acaccggctt gccttcgcgc gcctgcacgg
     1441 cggtcagcag acgcttttca tccagcgggc ggccggtttc gctggaccag cggctggcca
     1501 gatcttttac ctgggcagag agcttggccg gggcagcggc ttgcttgtag tggtcggcaa
     1561 acgccgtcac ccacagattg ccgttttcgt cgttgttcag cagcaccggc tggtaggtca
     1621 cggtcaccgg cacgcctttt tccatcagtg ctgccagctt gagcgcatcc gggctgcgca
     1681 ggcgcacgca gccatggctg cgaaagcccg gcacgctgga tggcacattg gtgccgtgca
     1741 tgcccaggcc cagcgcgggg tcaccaaagc ggatgaacac cggccccagc ggattgcccg
     1801 gccccggcgg cacggcggtt tctacatttt tgccggactg gcgcatttct gcctgaatcg
     1861 acgccggcac aaaccaggtg ggattacgcc gtatgtcggt gactttatat tcacccagcg
     1921 gggttttggt cagcgattta ccgacagcaa tcgggaacac atgggtgagt ttgccgtttt
     1981 ccagcacaaa caagcgggtt tgcggcacat tgataatcac ctgccgcccg ctgaccgtct
     2041 gagtgggcat cggcaagggg tcgaaggcgc cggcaaacgc gacgccagac agcaagcagt
     2101 acgacgaaag cagaatggtg gatttacgca tgacaagcca gtctcgggga gaatgcccgt
     2161 agtgtatcag tcatattcgt gcttgctgta gtccgcgctt ggtcatgacg ggaatttttt
     2221 accggaaacc aggtcgaaaa aggttggcaa ctgactctgc cgcagcggtg ttgcgcggat
     2281 tttcttttct tgaatacgag gtcgccatgc tgattcctcc ctccctgcgc cgcgctgcca
     2341 tcgaacttta cgacgcctac acccacacgc cgctaccccg gcgtgaattc ctgcgccggc
     2401 tgggcgtgct ggctggtggc acagtggcca ccagcagcct gctggccctg ctggaaaacc
     2461 actatgccca cgccgacacc gtgccagtgg acgatgcccg tctacagcat gccgatatcc
     2521 gctttgccca actggacggc cagccgctgc atggctacct gagccagccc gccagtggcg
     2581 ggccacggcc gggcgtggtg ctgattcacg aaaaccgtgg gctgaatccg catatccgcg
     2641 acgtggcgcg ccgactggcc ttggccgggt ttaccgtcct ggcgccggat tatctgtcta
     2701 cccagggcgg tacacccagc gatgaagacc gtgcccgcca ggcctttgcg gagatggatc
     2761 cggtgctggc ccggcgcatt tcactggccg cgctggccgc cctgcgtgaa cgcggcgcca
     2821 ccaaagtggg cgcactgggc ttttgctggg gcggtggcca ggtaggccag ctggcgatac
     2881 acgcggccga cttgcaggcc gcggtggtgt actacggccc gccaccggcc agcgatgagg
     2941 tggccgccat tcgggcgcca ctgctgttgc actacgccgg gctggacatc cgtatcaacg
     3001 ccatgctgcc ggccttcgag acggccttgc gcgcagcggg ggtccgccat cagctgtatc
     3061 gctacgcaaa cgtggatcat gccttcaaca atgacaccaa cccggcgcgc ttcaatgccg
     3121 aagctgccgc actggcctgg tcgcggagta tggcctttct gcatgcgcaa ctgggctagg
     3181 cccgcatgcc atcctgcgcc cgggcgggct ggatttttct gaaaacagcc gggttttttg
     3241 cgagaatagc ggtctttgcc cactggtagc agacccgagc atgacacacc cgcagccgat
     3301 cgcccgtatt gaatccctcg actttgaagg ccgtggcgta gcccacgtcg atggcaaaac
     3361 cctgttcatc gacggcggcc tgccgtatga gacggtgcgc tacagcagct accgcaaaaa
     3421 acccagctac gaaaacgccg acgcggtgca agtgctcaac gccagcttta tgcgcaccac
     3481 gccgcgctgc ccgcattttg gcgtgtgcgg cggctgttcg atgcagcatg tggaattctc
     3541 cgcccaggtg gccaataagc agcgcgtgct ggaagacaat ctggcccata ttggccgggt
     3601 gacgcccgaa gtcatcctgc cgccgattta cggccccacc tggggctatc gccaccgcgc
     3661 ccgcctgtcg gcgcgccatg tgccgaaaaa aggcggcgtg ctggtggggt ttcatgaaaa
     3721 gcgctccacc tatatcgccg acatgcgcga atgccatatt ctgcccgagc gcatttccgc
     3781 cttgctgatg ccgctgcgcg agctgatcgg gcaatggtcc atcgtggcgc gcatgccgca
     3841 ggtggagctg gcggtgggcg acgcggtgga tgtgctggtg ttccgcatca tggacccgct
     3901 gaccgccgcc gacgaggcct tgttgcgcca gtttatcgac gcccacgcca cgccgcagcg
     3961 cccgctgcaa gcctggctgc aacccaaggg cccggaaacc tgttatccgt tctacccgct
     4021 ggacgcgccg acgctgacct attcgctgcc ggattttcgc gtggtgatgc cctatcgccc
     4081 gaccgaattc acccaggtca accccggcat caaccgggtg atggtggcgc gcgccatgcg
     4141 cttgctggat gcgcagccgg gcgagcgcat tgccgatatg ttctgcggca ttggcaactt
     4201 caccctgccg attgcccgca gcggcgccac cgtgctgggc atggagggca gccagtcgct
     4261 ggtggagcgc gccatcgaca acgcccgcca caacggcctg gaggatcagg tcagctatcg
     4321 ggtggccaat ctgtttgaag tcaccccgga atcgtttgcc gcgctgggcc agtttgacaa
     4381 aatgctgatc gacccgccgc gcgacggcgc cattgccctg tgcaaggcgc tggacgaaga
     4441 caccgcgccg gcgcggctgg tgtatgtgtc gtgcaacccg gccaccctgg cgcgcgacgc
     4501 cggcgtgctg gtcaacacca agggctacac cctgcgcgcc gccggcatca tcaatatgtt
     4561 cccgcacacc ggccacgtag aaagcatcgc cctgtttgaa aaaaccggcc cgtgcgtcag
     4621 ccgcgccgag ctggaggcgc aagaggcggc cgaagccgcc gcccgcgaag ccgccaaggc
     4681 ggcggccaaa gtggcgcgcg ccgccaaaga agccgccgag gccgaagcgc gcgccgcccg
     4741 tgccgccgac aaggaagccc gccgtcaggc ctgggcagcc aaacgcgaag ccgaaggctg
     4801 gcacgaagcg tccgacgacg acacacaggg gaaggaataa gccaaggcgc gccagcctga
     4861 tattggcatg gcgcttgctt agagaaacgg gatgccgctc tcggcatcct ccactcgact
     4921 cggaagcgtg agctttggcc gccagcctgg caacaggctg gcgtttttta tgcgacaggc
     4981 cgatcaggca gcgctttgca tgctgggcaa aacacagtac gggtgtcgca acacgccaag
     5041 agggttttgt gcgcagcgct tattcgcgcc caggacgtgc aggcgtaccc tcggacaagg
     5101 tcagcacctc gcagccggtt tccgtcacca gcagggtgtg ctcccactgc gccgacaggc
     5161 tgcggtcctt ggtgaccacc gtccagccgt cgttgaggat tttcaggtga cgcttgccgg
     5221 cgttgatcat cggttcgatg gtgaaaatca tgcccggctg cagctcgacg ccggtgccag
     5281 cgcggccgta atgcagcact tgcggctctt catgaaaccc ccggccaatg ccgtggccgc
     5341 agaattcctg caccacgctg tagccgcagg cttcggcata tttctggatg gccgcgccaa
     5401 tatcgcccaa gtgggcgcca ggcttgacct gatcaatgcc aatccacagg cattcgtggg
     5461 tgacgcgcac caggcgcttg gcctgcggcg tggcctcgcc caccaaaaag gtgcggctgg
     5521 tgtcgccgtg atagccgtcc ttgatcacgg tgatgtccag attgacgata tcgccctttt
     5581 tcagcgcttt atcgttgggc acgccgtggc agatcacctg attgaccgag gtacagatgg
     5641 atttgggata cggcgcatgg ccttctggcg catagttcag cggcgccggg atgcagcctt
     5701 gctgattgac catatagtca tggcacaggc ggtcgagctc ttcggtggtg acgccgggct
     5761 gcacataggg ggtgatgtag tccagcactt cgctggccag ccggccggcc acgcgcattt
     5821 tggcgatgtc ttcggcgttc ttgatgatga taggcattga aaacggatac gaaagcaaaa
     5881 taatccatca tcttagcaaa aaaaacaggc cccggtgggg gcctgtctgg ctggcgtgtt
     5941 gcgtccggca tggcgcgctc acgcacgcct gtgcatcaga acttgtggct caggccgata
     6001 ccgaaggcgg tcgggttgga gccggcagcc acaccggtga tgccgttgct gtcaaagttg
     6061 atcttggcgg cgttcttgtt gtcgatgcgg gtgtagaaag cataagcctg agtgcgcttg
     6121 gacagatcat acgtgcccga cagcgaccat tggttggctt tgtaatccgc atcagaaatg
     6181 ctgccgctgc cagcgccctt gcgcttgccc aggctcacat aagccaggcc caggccaaag
     6241 ttgccaaact tctgggtggc ggacaccgac caggcggatt gcttttggtc agcgccgacg
     6301 atgcggtcct ggttttcgta ttcgtaaccc aggcccagtt cggtatcggc aaacttgtag
     6361 cgggccacgg ccttccagaa atcctgatga tccgtggtct tgctggcgcc ggccactgcg
     6421 ccggtatcgt cacgacgctc aaaagcgcca cccagcagca gaccatcggc gttgtactgt
     6481 gccgccagcg cccaggcttg ggcattggtg cgctcgctat tggccagcac ggcgcgagtt
     6541 tcatcggtac cgtaagaagc gccaaagctg aagccgctga aattcgggct gcggtattgc
     6601 acgctgttct tcatgcgctg ctgaccacgg ccgatgatgc cattggcgcc ggtaaagtcg
     6661 gccacgccat cttccagcgg aatcagatac ttgaccatct tgtaagcgct gtcgtagttg
     6721 cccagcatca cctggccaaa gcggccatcc aggccaacaa aggtattgcg ggtagcccag
     6781 ttgcccttgc gggtgccgcc atcgtcgatg gagacttctt gctcaacctg ccaaatggcc
     6841 ttcaggccat tgcccagatc ttcattgcct ttgaaaccga tcttggaggt gttgctgctg
     6901 acgcgggagg tggatttgac gtcattggcg gaattggccg cgccagtggc tttgaccgat
     6961 tccagcgagt tattcagggt gccgtaaatg gtcacgtcag ccagggccag cggggaagca
     7021 cacgctgcgc tcagcgcaac gacaagcatt ttgcggttca tgggggtacg ctccagggga
     7081 aaaaacgcag aggtgttgca agcaacacag gacagccgcg agtttacgga gcgtagatga
     7141 cgagggtgtg gtgaaaatgt tacggtgaaa gcgcagcttg ctgcgcaatc ggcctgatgt
     7201 tgtcgtctgc acgcaacaac agaccagaaa cacgccaaca gcgcgcctgg ccaggtgtgg
     7261 cggtgtgaca cggcaaagcc acgccacacc gccaggcgcc tcttggcgcg gctaaaaaaa
     7321 ccccagcgca gcgagcctgg ctaagggcac gaccgcaaac agtacaacaa gcacgataag
     7381 gtcaggcaac aacgccagga gggttttgta agccgtcctt atgctgccgg cgcttgctgg
     7441 gtcaactcct caggcaagca gccaatcggc gggcccagct cgccgctggc gtcttcaaag
     7501 cgcaccagat aaatcatctg gctttcgtct tcttcggcgt agccagactg caccaccacg
     7561 ccgcgcgcgc cagccggcgc cagaatggcg tcttcgtcag cgccgggcat gccgccatcg
     7621 ttgaacagat cttcggcgca atacaccatc tcgccgactt cataagggat cacaggcagc
     7681 gggggcaaca tggcatcagc tccagacatc gggcggcgcc aacagacgct caaccggttg
     7741 atcaaacatc aggtgttcat caatgatcac gcccacgtct tcaggtttga cgccggtgta
     7801 catcacgcct tccgggtaga caatcaccgt tggccccaga tgacagggcc ccagacagcc
     7861 ggtgttggtc agcaggaagt tggcccacag attgcgttgc tggaattcat tggaaaacgc
     7921 ctgaaacaca tcattgcagc cacgggcctg acaggagccg cgcgggtggc cgggtgggcg
     7981 gccctgaacg cagaccagaa catgtttttt gggcttgggc atgatggggt tcctgttgga
     8041 cagaggggat atgtctgtca cagcaaggga gatgccagcc ataagctttt gtttttacaa
     8101 caagccatac aaggcctgtc agaaacccca caatctggcg cgcaaaaaaa aacagagccc
     8161 cgaagggctc tgttttttct gaattcagct gcgccaccag gacgcggatg aattagaagg
     8221 cgtggcggat gccaacaccg atggcttgcg gatcagcacc agcggcggac agggtgccgt
     8281 tcagcgggtt gataccgaag ttgcggttag ccgacttgtt gttgctcagc ttggttgcgt
     8341 aagcttgcac ttgggtgcgc ttggacaggt cgtaggtagc agccagaacc cattgcttgg
     8401 ccttgccgtc gccttcagca ccgttctttt ccttgcccag ccaggtgtag gaagcgccca
     8461 cgccccatgc gcccagctgt tgcttgacgg ccagggtgaa gccatcttgc ttcttggcgg
     8521 acttggcgaa ttggtccact tcgctgtgct cgtagccggc agccagggtg gtggcggaag
     8581 cgaaggtgta agcgccaacc agcttgaagt agtcggtgtc cagaccggtg gcggtagcca
     8641 cgctgtcgtt gcggtgttcg tagccagcac ccacagccag gccgccgttc acgtacttgc
     8701 cgccgaagga cagcgagtag gcgttagcgc ggttgccgcc cacggtagcg cgggtttcgt
     8761 cagcaccgta ggacacgccg gcgctgaagc cgttcaccac cgggctgtag tattgcacgg
     8821 tgttgctcac gcgctggtcg ccacggcaga agatggcttg gctggtgcag gtatcagccg
     8881 aggtgtcggc caggatgttg gtgcccagac cgatggtcat ggtcttgtac gcgctgtcat
     8941 gcttgcccag cagcacgcgg ccgaagtcgc cttgcaggcc gatgaagctg ttgcggttag
     9001 cccagctggt gctggagttg ccggtgtcga tgtcgacgtt ttgttcaact tgccagatgg
     9061 ccttcaggcc gttgcccaga tcttcccagc ccttgaagcc gatgcgggag ttgttggcgg
     9121 tcacgcggtt gaccgacttc acgtccagcg cagagttggt ggcgccagtc gccttggcaa
     9181 attccacgga ggagttgatc acaccgtagg tggtcacatc agcgaaagca gccggggcaa
     9241 caaaggcagc ggacagggca gcagcaagca gtttcttgtt catagggttt cagctccaca
     9301 gggttgtcgt gatggactgt gccgctttga atatgcggtt tctttacaaa tccatggcgg
     9361 atgctaacag agcttgacga ggagggcggc aaagttttgt tgtatttttg cgcggcattg
     9421 c
//
LOCUS       DAOPYX010000100         9367 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_8658_length_9367_cov_9.952749, whole genome shotgun sequence.
ACCESSION   DAOPYX010000100 DAOPYX010000000
VERSION     DAOPYX010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 9367)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9367)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9367
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8658_length_9367_cov_9.952749"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            203..1522
                     /locus_tag="PKN55_06695"
     CDS             203..1522
                     /locus_tag="PKN55_06695"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing methyl-accepting
                     chemotaxis protein"
                     /protein_id="HNX70964.1"
                     /translation="MFNQKLKQEISALQAQLQALTAQESAVSRAMAMIRFDRDGKVVN
                     ANPLFLQTMGYSLADLQGKPHSQLCFPDYANSADYRAFWDRLRRGEPFSDRVRRRTAD
                     GREIWLEATYCPVRDAQGQIESFSKYASDITASVDAEVHNQARLNAINRAMAVIEFSP
                     DGTVLDANENFLNVLGYRLDEVKGRHHRLFCDNEFAQSPEYQALWRQLARGEYYSGQI
                     KRQAKNGAVRWLEASYNPVFAPDGKTVIAVVKFASDITERVESQRQESESARFAYEVA
                     QETEALSSTGADNIRNSSDEIAQMAGSIEEAGHSVQALGERSQQITSIVQTIKDIADQ
                     TNLLALNAAIEAARAGETGRGFAVVADEVRKLAERTAASTAEISQMVSDIQTQTQVAV
                     QNMSEIREQATQSVNRTREAGETMDQIMEGARSVVKAISQYAAGQRA"
     gene            1519..1998
                     /locus_tag="PKN55_06700"
     CDS             1519..1998
                     /locus_tag="PKN55_06700"
                     /inference="COORDINATES: protein motif:HMM:NF024871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNX70965.1"
                     /translation="MSASLRLATPADIPALSALLSVLFAQEADFQPDPAKQAAGLELL
                     FARQLGDILLAEADGAVRGMVSLLYTVSTALGGPVAWLEDMVIDPAWRNAGLGGLLLD
                     AALAHAQQQGCLRVTLLTDADNHAAQRFYQGRGFSPSAMQPWRRVFAAPPAPHPENG"
     gene            complement(1925..2833)
                     /locus_tag="PKN55_06705"
     CDS             complement(1925..2833)
                     /locus_tag="PKN55_06705"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HNX70966.1"
                     /translation="METIQPWEGVVEFVTVAEQHSFTAAARKLGMSVAQVSRQVSALE
                     ARLATRLLYRTTRQVSVTEAGQLYYQHCRPLLDGLLAAEQALSLSQAHPSGHLRLTAP
                     VYYGETVIAPLLLQYLAHHPAVSAELNLDNRKVDLVAENFDLGIRLGPMDGSSLMLRE
                     LAVRVHHVCASPAYLARHGAPQTLAELRQHACLVGTVESWRFLDSGQVREVHVSGPLR
                     CNSGVALRAAALAGMGLVQLPDYYVDEALARGELMPVLERYTLRDAVWAAWPRSRFTP
                     PKVSRFLDEVQAALQTPAARAAWPMG"
     gene            2959..4086
                     /locus_tag="PKN55_06710"
     CDS             2959..4086
                     /locus_tag="PKN55_06710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011134295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase activity,
                     zinc-dependent [Evidence IEA];
                     GO_function: GO:0051903 - S-(hydroxymethyl)glutathione
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA];
                     GO_process: GO:0009404 - toxin metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase/class
                     III alcohol dehydrogenase"
                     /protein_id="HNX70967.1"
                     /translation="MTMIKTRAAVAWAPNQPLTIEEVDLMPPQKGEVLVRIVASGVCH
                     TDAYTLSGKDSEGVFPCILGHEGAGVVEAIGEGVTSVAVGDHVIPLYTPECGECKFCK
                     SGKTNLCQAIRATQGKGLMPDGTTRFYKDGKPIYHYMGTSTFSERTVLPEIALAKINK
                     EAPLEEVCLLGCGVTTGMGAVMNTAKVKAGDTVAIFGLGGIGLSAVIGARMAGASRII
                     GIDINTSKFDLAKQLGATDVINPNDFSKPIQDVIVEMTDGGVDFSFECIGNVHVMRSA
                     LECCHKGWGESVIIGVAGAGEEIATRPFQLVTGRVWRGSAFGGVRGRSELPNYVERYL
                     KGEFRLDDFITHTMPLEDINTAFDLMHEGKSIRTVIHFDAK"
     gene            4094..4942
                     /gene="fghA"
                     /locus_tag="PKN55_06715"
     CDS             4094..4942
                     /gene="fghA"
                     /locus_tag="PKN55_06715"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018738 - S-formylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0046292 - formaldehyde metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HNX70968.1"
                     /translation="MSATLELVSSQRCFGGWQRRFRHASASTRCEMTFSVYLPPQAEA
                     GARVPALYWLSGLTCTDENFSTKAGAQRAAAELGLALVIPDTSPRGAHVADAERYDLG
                     QGAGFYLNATQAPWSEHYRMEDYIVDELPALLEAELPIGPLRAISGHSMGGHGALTLA
                     MRHPGRYVSASAFAPIVNPADTPWGQQAFAAYLGEAREAWLAHDASQLMATVAHRLPL
                     RVDQGGGDSFLPEQLQPEVLCAAAKAHGWTLDYTLHPDYDHSYYFIASLIEAHLRFHA
                     GHLARA"
     gene            complement(4987..5766)
                     /locus_tag="PKN55_06720"
     CDS             complement(4987..5766)
                     /locus_tag="PKN55_06720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018150884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HNX70969.1"
                     /translation="MESTESESKDRLFVTALARGLAVLAAFRGGEPQLSNQDLASRTG
                     LPKSTVSRLTYTLTQLGYLDQDPASGRYQLGLAVLELSAATLASFDARRVIAPKMQQF
                     AEAHGVSVSLAVREGLDMMYLETVRSRARVTVQLSVGSRVPLATTAIGRAYLAAMPAA
                     ERNTLMPALAVQYGADWPQIAASLDAALADYQQRGYCSSFGEFQPEVFAVAAPVPPLA
                     PGQPWMSLNASGPAFLFTESAMRERIAPPLLALVRELGARQ"
     gene            6002..7183
                     /locus_tag="PKN55_06725"
     CDS             6002..7183
                     /locus_tag="PKN55_06725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009118411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HNX70970.1"
                     /translation="MSNSRPAFQWDDALLLDAQLTEEERMVRDSARAYCQDKLLPRVL
                     TANREERFDREIMSEMGELGLLGCTLDNWGCAGLSHVAYGLVAREVERVDSGYRSAMS
                     VQSSLVMHPIYAYGTDAQRDKYLPKLATGEWVGCFGLTEPDAGSDPAGMKTRARKVDG
                     GWTLNGSKMWITNSPIADVCVVWAKDDEGEIRGFILDRGMPGLSMPKIHGKFSLRASI
                     TGEIVMDNVFVPDDNLLPNVKGLKGPFGCLNKARYGIAWGAMGAAEFCWHAARQYTLD
                     RKQFNRPLAATQLVQLKLANMQTEITLGLQAALRVGRLMDEGQAAPEMISLIKRNNCG
                     KALDIARVSRDMHGGNGIADEFHVIRHVMNLEAVNTYEGTHDVHALILGRAQTGIQAF
                     G"
     gene            7568..8758
                     /locus_tag="PKN55_06730"
     CDS             7568..8758
                     /locus_tag="PKN55_06730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014085822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HNX70971.1"
                     /translation="MAGALDGIKVLDLSRVLAGPWASQLLADLGATVIKVEKPGAGDD
                     TRHWGPPNLPDGTAGYFLAANRGKQSITVDITQPEGRAIVQQLAAEADVLLENYKVGG
                     LKKYGLDYPTLKAINPRLVYCSITGFGQTGPYASRPGYDYIVQGMSGLMSITGPADGE
                     PHKVGVAVTDLFTGLYASNAICAALLARERSGEGQAIDLALFDCALAMLANINMNWLV
                     GGETPPRLGNAHPNIVPYQVFATSDGHFILACGNDSQFARIAELCGHAEWAADPRFAR
                     NADRVAHRAELVPLLAEVFVTRTRDEWLAALDAAGVPNGPINTVDQAFADPQIVARGM
                     RLDLPDAQGAPVPQVACPIQLSGTPVVYHAAPPALGQHTEAVLMTLGKSAEEIAQLRQ
                     NGVV"
     gene            8988..>9367
                     /locus_tag="PKN55_06735"
     CDS             8988..>9367
                     /locus_tag="PKN55_06735"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017507262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNX70972.1"
                     /translation="MEYDVVIVGGGPSGLSAAIRLKQLAEQHGRELSVCLLEKGSEIG
                     AHILSGAVLEPTALNELIPDWKDKGAPLNTPAGEDRFLHLTETEATRLPTPPQMNNHG
                     NYIISLGNFCRWLGEQAEALGVEI"
BASE COUNT         1816 a         3076 c         2938 g         1537 t
ORIGIN      
        1 gttggtttga cgagacaatg ccacaaggct atgctggcgc gcgcatacac aagtctcatg
       61 caaagcagcc ttggctaggc acagcacaag ctacggctag gtcacacaac aacactaggg
      121 cgttttgtta gctgctctta agcatggcta gcagctagca cgctaccccg ccatcaagcc
      181 tgatcctctc ggaaaatcaa ctatgttcaa tcaaaaactg aagcaggaaa tcagcgcact
      241 gcaagcccaa cttcaggcgc tgaccgctca ggaaagcgcg gtatcacgcg ccatggcgat
      301 gatccgcttt gatcgcgatg gcaaggtggt caacgccaac ccattatttc tgcaaaccat
      361 gggctacagc ctggccgatc tgcaaggcaa gccgcacagc cagctgtgct ttcccgacta
      421 cgccaatagc gcggattacc gcgccttctg ggatcgcctg cgccggggtg agccgttcag
      481 cgaccgggtg cgccgccgca ctgccgacgg ccgggaaatc tggctggaag ccacctactg
      541 tccggtgcgc gatgctcagg gccaaattga atcgttcagc aaatatgcct cagacatcac
      601 cgccagcgtg gatgccgaag tacacaacca agcccggctg aacgccatca accgcgccat
      661 ggcggtgatt gaattcagcc cggacggcac ggtgctggac gccaatgaga attttctgaa
      721 tgtgctgggc taccggctgg acgaggtcaa gggccgccac caccgcctgt tttgcgacaa
      781 tgaatttgcc caatcgccag aataccaggc gctgtggcgc cagctggcgc gcggcgagta
      841 ttactctggc cagatcaagc gccaggccaa aaacggcgcg gtgcgctggc tggaagccag
      901 ctacaacccg gtgtttgccc cggacggcaa gacggtgatc gccgtggtca agtttgcctc
      961 ggacatcact gaacgggtgg aatcgcaacg ccaggaaagc gaaagcgcgc gctttgccta
     1021 cgaagtggcg caagaaaccg aagcgctgtc cagcaccggc gccgacaata tccgcaacag
     1081 cagcgacgaa atcgcgcaaa tggccggcag catcgaagaa gccgggcata gcgtgcaggc
     1141 gctgggcgaa cgctcgcagc aaatcacctc gattgtgcaa accatcaagg acattgccga
     1201 ccagaccaat ctgctggcct tgaacgccgc catcgaagcc gcccgcgccg gcgaaaccgg
     1261 ccgtggcttt gccgtggtgg ccgacgaagt gcgcaaactg gccgaacgca ccgccgcctc
     1321 caccgctgaa atcagccaaa tggtgtccga catccagacg caaacccagg tggcggtgca
     1381 aaacatgagc gaaatccgcg agcaggccac gcaaagcgtt aaccgcaccc gcgaagctgg
     1441 cgaaaccatg gaccagatca tggaaggcgc gcgctcggtg gtgaaagcca tcagccagta
     1501 cgccgccggc cagcgcgcat gagcgccagc ctgcgcctgg ccaccccggc cgacatcccg
     1561 gcgctgagcg ccttgctcag cgtgctgttt gcccaggagg ccgactttca gcccgacccg
     1621 gccaaacaag cggccgggct ggagctgttg tttgcccgcc agctgggtga cattctgctg
     1681 gcggaggccg atggcgcggt gcgcggcatg gtgtcgctgc tgtacaccgt cagcaccgcg
     1741 ctgggcgggc cagtcgcctg gctggaagac atggtgatcg acccggcctg gcgcaatgcc
     1801 ggcctgggcg gcctgctgct ggacgccgcg ctggcgcacg ctcaacagca aggctgtctg
     1861 cgcgtcaccc tgctgaccga cgccgacaac cacgccgccc agcgctttta ccaggggcgc
     1921 ggctttagcc catcggccat gcagccctgg cggcgggtgt ttgcagcgcc gcctgcacct
     1981 catccagaaa acggctgacc ttgggcgggg tgaagcggct gcgcggccag gccgcccata
     2041 ccgcatcgcg cagggtgtag cgctccagca ccggcatcag ctcgccgcgc gccagcgcct
     2101 cgtccacata gtaatccggc aattgcacca gccccatccc cgccagcgcc gccgcgcgca
     2161 gcgccacgcc gctattgcag cgcaagggcc cgctgacatg cacctcgcgc acctgcccgc
     2221 tatccagaaa acgccagctt tccaccgtgc ccaccagaca ggcgtgctgg cgcagctcgg
     2281 ccagcgtctg cggcgcgccg tggcgggcca gataggccgg cgaggcgcac acatggtgca
     2341 cgcgcaccgc cagctcgcgc agcatcaagg aactgccgtc catcggcccc aggcgaatcc
     2401 ccagatcaaa attttccgcc accaaatcca ccttgcggtt atccagattc agctcggcgc
     2461 tgaccgccgg gtgatgggcc agatactgca agagcaaggg cgcaatcacc gtctcgccgt
     2521 aatacaccgg cgcggtcagc cgcaaatggc cgctggggtg ggcctggctg agcgacagcg
     2581 cctgctcggc ggccaacagg ccatccagca gcggccggca gtgctggtaa tacaactgcc
     2641 cggcctcggt gacgctgacc tgacgggtgg tgcgatacag caaacgggtg gccagccgcg
     2701 cctccagcgc cgacacctgg cggctgacct gcgccaccga catgcccagc ttgcgcgctg
     2761 cggcagtaaa gctgtgctgc tcggccacgg tgacgaactc aaccacgcct tcccagggct
     2821 ggattgtttc catagagtaa taatgtcttt ccgtaaacgg ggattatcgc gtgcagggca
     2881 gagattacaa tactggcatg ctgcgcgccg ctacgcccgc gcccgacact tttgattgcc
     2941 ttcgcacagg ataccgccat gaccatgatc aaaacccgcg ccgccgtcgc ctgggcgccg
     3001 aaccagccgc tgaccatcga agaagtggac ctgatgccgc cgcaaaaagg cgaggtgctg
     3061 gtgcgcatcg tcgccagcgg cgtatgccac accgacgcct acaccttgtc cggcaaggat
     3121 tccgaaggcg tgttcccctg cattcttggc catgaaggcg ctggcgtggt ggaagccatc
     3181 ggcgagggcg tcaccagcgt ggcggtgggc gaccatgtga ttccgctgta caccccggaa
     3241 tgcggcgagt gcaagttttg caaatccggc aagaccaatc tgtgccaggc catccgcgcc
     3301 acccagggca agggcctgat gccggacggc accacccgtt tttacaaaga cggcaagccg
     3361 atttaccact atatgggcac ctcgaccttc tccgagcgca ccgtgctgcc agaaatcgcc
     3421 ctggccaaga tcaataaaga agccccgctg gaagaagtct gtctgctcgg ctgtggcgtc
     3481 accaccggca tgggcgcggt gatgaacacc gccaaggtga aggccggtga cacggtggcg
     3541 atttttggtc tgggcggcat cggcctgtcg gcagtgattg gcgcgcgcat ggctggcgcc
     3601 agccgcatca tcggcatcga catcaacacc agcaaatttg acctggccaa gcaactgggc
     3661 gccaccgacg tgatcaaccc caatgacttc agcaagccga ttcaggacgt gattgtcgaa
     3721 atgaccgacg gcggggtgga tttttcgttt gaatgcatcg gcaatgtgca tgtgatgcgc
     3781 tcggcgctgg aatgctgcca caagggctgg ggcgaatcgg tgattatcgg cgtggccggc
     3841 gctggcgaag aaatcgccac ccgcccgttc cagctggtca ctggccgggt gtggcgcggc
     3901 tcggcgtttg gcggcgtgcg cggccgctcc gagctgccga actatgtcga acgctacctg
     3961 aaaggcgaat tccgcctgga cgacttcatc acccacacca tgccgctgga agacatcaat
     4021 actgcgtttg atttgatgca cgaaggcaag tcgatccgca cggtgattca ctttgacgcc
     4081 aagtgagccg ccaatgagcg ccacgcttga attagtgtcc agccagcgct gctttggcgg
     4141 ctggcagcgc cgctttcgcc acgccagcgc cagcacccgc tgcgagatga ctttttcggt
     4201 gtacctgccg ccgcaggcgg aggccggcgc gcgcgtgccg gcgctgtact ggctgtccgg
     4261 gctgacctgc accgacgaga atttttccac caaggccggc gcccagcgcg ccgccgccga
     4321 gctggggctg gcgctggtga tccccgacac cagcccgcgc ggcgcgcacg tagccgacgc
     4381 cgagcgctac gatctgggcc agggcgccgg gttttacctg aacgccacgc aagcgccctg
     4441 gagcgagcac taccggatgg aagactatat cgtcgatgag ctgccggcgc tgctggaagc
     4501 cgaactgccg attggcccgc tgcgcgccat cagcggccac tcgatgggcg gccacggcgc
     4561 gctgaccctg gccatgcgcc accccggccg ctatgtctcg gcgtcggcct tcgcccccat
     4621 cgtcaacccg gccgacaccc cgtggggcca gcaggcgttt gccgcctacc tgggcgaggc
     4681 gcgcgaggcc tggctggcgc acgacgccag ccaactgatg gccacggtgg cgcatcgtct
     4741 gcccttacgg gtggaccaag gcggcggcga cagctttttg ccggaacagc tgcaacccga
     4801 ggtgctgtgc gccgcggcca aggcgcatgg ctggacgctg gactacaccc tgcacccgga
     4861 ctacgaccac agttattact tcatcgccag cctgattgag gcgcacctgc gctttcatgc
     4921 cgggcatctg gcccgggctt gagcctgtac ggatgacagt caaccgccgg cttgccggcg
     4981 gtttttttat tgccgcgcgc ccaactcgcg caccaaggcc aacaacggtg gggcgatgcg
     5041 ttcgcgcatg gcgctttcgg taaacaaaaa cgccgggccg ctggcgttca ggctcatcca
     5101 gggttggccg ggggccagcg gcggcacggg ggcggccacg gcaaacactt cgggctgaaa
     5161 ctcgccaaac gagctgcaat agccgcgctg ttgataatcc gccagcgcgg cgtccaggct
     5221 ggcggcaatc tgcggccagt cggcgccgta ttgcaccgcc agcgccggca tcagcgtgtt
     5281 gcgctcggcc gccggcatgg cggccaggta ggcgcggcca atcgccgtgg tggccagcgg
     5341 cacgcgcgaa cccacgctga gctgcaccgt cacccgcgcg cggctgcgca cggtttccag
     5401 atacatcatg tccagccctt cacgcaccgc caggctgacc gacacgccat gcgcctcggc
     5461 aaattgctgc attttgggcg caatcacccg gcgcgcgtca aagctggcca gggtggcggc
     5521 ggacaattcc agcaccgcca ggcccagttg ataacggccg ctggccgggt cttgatccag
     5581 ataacccagt tgcgtgagcg tgtaggtcag ccgcgacacc gtgcttttgg gcaggccagt
     5641 gcggctggcc aggtcttgat tggacaattg cggctcgccg ccgcgaaacg ccgccagcac
     5701 cgccagcccg cgcgccagcg cggtgacaaa caggcggtcc ttgctttcag attctgttga
     5761 ttccataggg tgtccatacg tcgggcgcgc tgtatggcgc gcgcaaagtg accatggcgc
     5821 aggctgacat ccgcccgcgc acttggccat gcgtcatcct gccactttac atcaggctaa
     5881 ggtttggcta aagtgttctg cattgtagaa cattattccg caaagcggaa caacaaccac
     5941 ccgatgcgcc agcacacccc gcccggcgca cccgacccga ctgattgcaa ggagacccgt
     6001 catgtccaat tctcgcccgg cgtttcaatg ggacgatgcc ctgctgctgg atgcgcaact
     6061 cactgaagaa gagcgcatgg tgcgcgacag cgcccgcgcc tactgtcagg acaagctgct
     6121 gccgcgcgtg ctgaccgcca accgcgaaga gcgcttcgac cgcgaaatca tgagcgagat
     6181 gggcgagctg ggcctcttgg gctgcacgct ggacaactgg ggctgcgccg gcctgagcca
     6241 tgtggcctac ggcctggtgg cgcgcgaagt cgagcgggtg gattctggct atcgctcggc
     6301 aatgagtgtg caaagctcgc tggtgatgca cccgatttac gcctacggca ccgatgccca
     6361 gcgcgacaaa tacctgccca agctggccac cggcgagtgg gtgggctgct ttggcctgac
     6421 cgagccggac gccggctccg acccggccgg catgaaaacc cgcgcccgca aggtggacgg
     6481 cggctggacg ctgaacggca gcaagatgtg gatcaccaac agcccgattg ccgatgtgtg
     6541 cgtggtgtgg gccaaggacg acgagggcga aatccgtggt tttattctgg atcgcggcat
     6601 gccgggcctg agcatgccga agattcacgg caagttctcg ctgcgcgcgt cgatcaccgg
     6661 cgaaatcgtc atggacaatg tgtttgtgcc ggacgacaac ctgctgccga atgtcaaagg
     6721 cctgaaaggc ccgtttggct gcctgaacaa ggcccgctac ggcatcgcct ggggcgccat
     6781 gggcgcggcg gaattctgct ggcacgccgc ccgccagtac acgctggacc gcaagcaatt
     6841 caaccgcccg ctggccgcca cccaattggt gcaactgaag ctggccaata tgcagaccga
     6901 aatcaccctg ggcctgcaag ccgcgctgcg cgtgggccgg ctgatggacg aaggccaggc
     6961 cgcgccggaa atgatcagcc tgatcaagcg caataactgc ggcaaggcgc tggacatcgc
     7021 ccgcgtcagc cgcgacatgc acggcggcaa cggcattgcc gacgaattcc acgtcatccg
     7081 ccatgtgatg aacctggaag cggtgaacac ctatgaaggc acgcacgatg tgcatgcgct
     7141 gattctgggt cgggcgcaga ccggcattca ggcatttggc taaacgccga gccaaggcca
     7201 cttcgctggg ttttgtgggc gacgccaaac gcagctgcgc cggtcagccg ccagcggatt
     7261 aacccaaagc ccggccgccc ttgcgccgtc gggcgtcagc aagatcatgc gcggtcgagg
     7321 gagtttccct ccatcatctt tgcccggcct gcctcaggtc cgggcgtttt tttcggtttt
     7381 tttgcgaggc tccctcatcc cccttggcta agagtggcta acaaaactca gcgcagcgtt
     7441 cctggctagg cgcacgcaga cagtgcgggt agtgcggcaa ggtcgggcaa ccacgccagg
     7501 agggttttat tagccacgct aaaacagaca acacggagcg ggtgagtgag cctgaagagg
     7561 agtcaccatg gctggagcat tagacggcat caaagtgctg gatttgtcac gggtgctggc
     7621 cgggccttgg gccagccagt tgctggcgga tttgggcgcc accgtcatca aagtagaaaa
     7681 acccggtgcc ggtgacgaca cccgccactg gggcccgccc aatctgccgg atggcaccgc
     7741 cgggtatttt ctggcggcca accggggcaa gcagtcgatc accgtggaca tcacccagcc
     7801 ggaagggcgg gcgattgtgc agcaactggc cgccgaagcc gatgtgttgc tggaaaacta
     7861 caaagtgggc ggcctgaaaa aatacgggct ggactacccc accctcaagg ccatcaaccc
     7921 gcgcctggtg tattgctcga tcaccggctt tggccagacc ggcccctacg ccagccgccc
     7981 cggctacgac tacatcgtgc agggcatgag cgggctgatg agcatcaccg gcccggccga
     8041 cggcgagccg cacaaggtgg gcgtggccgt caccgacctc tttactggcc tgtacgccag
     8101 caacgccatc tgcgccgccc tgctggcgcg cgagcgcagc ggcgaaggcc aggcgattga
     8161 tctggcgctg tttgactgcg cgctggccat gctggccaat atcaatatga actggctggt
     8221 gggcggcgaa accccgccgc gcctgggcaa tgcccacccg aacatcgtgc cgtaccaggt
     8281 gttcgccact tctgatggcc attttattct ggcctgcggc aacgacagcc agtttgcccg
     8341 cattgccgag ctgtgcggcc atgccgaatg ggccgccgac ccgcgctttg cccgcaacgc
     8401 cgaccgcgtg gcgcaccgcg ccgagctggt tccgctattg gccgaggtgt ttgtcacccg
     8461 cacccgcgat gaatggctgg ccgcgctgga cgccgccggc gtgcccaatg gcccgatcaa
     8521 taccgtagac caggcgtttg ccgatccgca aatcgtcgcg cgcggcatgc ggctggattt
     8581 gccggatgct cagggcgcgc ccgtgccgca ggtggcgtgc ccgattcaac tgtccggcac
     8641 gccagtggtg taccatgccg ccccaccagc cttggggcag cacaccgagg cggtgttgat
     8701 gaccttgggg aaaagcgccg aagagattgc ccagctgcgg caaaacggcg tggtgtagcc
     8761 cgcgcgccgc cacggcgtga catccggcgc ggccgatggc gcgcgcccac ttccccagcg
     8821 tcactggcaa gcagtggcat gcttgccgca cgaggaaccc ggctcgggtc aaatttcaaa
     8881 cgaccgtttg acccgccggg catttgcatg catgattcgc gcaacacacc ctcaaaaaaa
     8941 cagctgttgc ggcggatgac aaggagactg cggtggaacg cgaatatatg gaatacgatg
     9001 tggtgattgt cggcggcggc ccatccggac tgtcggcggc gattcgcctc aagcaactgg
     9061 ccgagcaaca cggccgcgaa ctcagcgttt gcctgctgga aaaaggctcg gaaatcggcg
     9121 cccatatttt gtcgggcgcc gtgctggagc ccaccgcgct caatgaactg attcccgact
     9181 ggaaagacaa aggcgcgccg ctgaacaccc cggccggcga agaccgcttt ttgcacctga
     9241 ccgaaaccga ggccacccgt ctgcccacgc cgccgcagat gaacaaccac ggcaactaca
     9301 tcatcagcct gggcaatttc tgccgctggc tgggcgagca ggccgaggcg ctgggcgtgg
     9361 aaatcta
//
LOCUS       DAOPYX010000101         9314 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_8726_length_9314_cov_12.266983, whole genome shotgun sequence.
ACCESSION   DAOPYX010000101 DAOPYX010000000
VERSION     DAOPYX010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 9314)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9314)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9314
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8726_length_9314_cov_12.266983"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..718)
                     /locus_tag="PKN55_06740"
     CDS             complement(<1..718)
                     /locus_tag="PKN55_06740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HNX70973.1"
                     /translation="MQNISAVLYLVSAVLFILSLKGLSSPASSRKGNTYGMIGMLIAV
                     ATTFMVMEKPVLLLIVGAIAAGAAIGAFKAKTVEMTSMPELVAAMHSLVGLSAVLIAV
                     AAIFHSGMEHTPVQKVELFIGAFIGAITFTASVIAYGKLSGKFGAKAVNFNGQHLLNL
                     VMALAMVGFGGVYFFTDSHAAFLIMVAIALVLGVTLIIPIGGADMPVVVSMLNSYSGW
                     AAAGIGFTLNNPVLIIAGACV"
     gene            complement(729..1022)
                     /locus_tag="PKN55_06745"
     CDS             complement(729..1022)
                     /locus_tag="PKN55_06745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-translocating transhydrogenase family
                     protein"
                     /protein_id="HNX70974.1"
                     /translation="MTDPFIISLTIFVLAVFVGYHVVWNVTPALHTPLMAVTNAISGI
                     IIVGAMLQVVDINGQTLTVTSVLGAVGIFLASINIFGGFLVTQRMLDMFKKKK"
     gene            complement(1050..2171)
                     /locus_tag="PKN55_06750"
     CDS             complement(1050..2171)
                     /locus_tag="PKN55_06750"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011133656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008746 - NAD(P)+ transhydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HNX70975.1"
                     /translation="MQIAIPAELAAGETRVAATPDTVKKYVALGHAVAVQSGAGRASA
                     VLDDAYIAAGATVVGDRAALLAAADIVLTVRALPAEDIPSLKAGAVLIGMLDPHRNNT
                     LAELASRQISAFTMELLPRTTRAQSMDVLSSQNNIAGYRAVLLATQFYPRFMPMLMTA
                     AGTVKAAKVLVLGAGVAGLQAIATAKRLGAVVEAFDVRPATKEQVESLGGKFVEVPMT
                     DEEKKANDGVYAKEMSDDYKRRQGELVAKHAKAADIVITTALIPGKQAPILLEADVVA
                     GMKPGAVIVDIAAESGGNCPLTRPGAAFTTDGGVTVVGFTNLPGMVAADASSLYAKNL
                     LTFLSLMLTKEGFSLNLEDDLLAATLITHQGVLRFGQAA"
     gene            complement(2731..3492)
                     /locus_tag="PKN55_06755"
     CDS             complement(2731..3492)
                     /locus_tag="PKN55_06755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011136098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HNX70976.1"
                     /translation="MSESLPFLGLMAFMAGLIDAAVGGGGLIQIPALFNALPHVAPAT
                     LFGTNKVAAVSGTLFAARSYVRQVRLPWKLVLPAALAAFAFSFVGASVVSWIPKSVMR
                     PLVLVLLLLMAVYTLWKKDFGALHAPVSIGRRERVMATALGGAIGFYDGVFGPGTGSF
                     LIFLFIRCFAFDFLHASAAAKFVNIATNIAALSYFLPQDQVLWHYALPMAACNVLGAI
                     SGSWLALRGGSQVVRVLFLVLLTVLIGRFAWEVLG"
     gene            3962..>5175
                     /locus_tag="PKN55_06760"
     CDS             3962..>5175
                     /locus_tag="PKN55_06760"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: protein motif:HMM:NF001221.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HNX70977.1"
                     /translation="MDIQAYMLDLGRAARAASRQLARTDTAQKNRALLAIADALQRDS
                     DALVTANQADLAAARDAGLEPAMLDRLTLSAAGVAQMAEGLRQIAALPDPVGEMGEFA
                     YRPSGIQLGKMRVPLGVIGIIYEARPNVTADAAGLCLKSGNACILRGGSEAIRSNQAI
                     AACVHEGLREAGLPEGAVQVINTTDRATVGALVTMTEFVDVIVPRGGKGLIARVSAEA
                     RVPVIKHLDGNCHVYVDVDADLVKAGRIADNAKTQRYGTCNTMETLLTHHTVAAEFLP
                     VIAAIYLSKGVELRGCPRAQEIIGAQVKPASEDDWFTEYAAPIVALKVVDSLDEAIEH
                     INHYGSHHTDAIVTENYTTARRFLREVDSSSVMVNASTRFADGFEYGLGAEIGISTDK
                     IHARGPVGLHGLT"
     assembly_gap    5176..5275
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            5279..6349
                     /gene="dprA"
                     /locus_tag="PKN55_06765"
     CDS             5279..6349
                     /gene="dprA"
                     /locus_tag="PKN55_06765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011137813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HNX70978.1"
                     /translation="MHCLLTDGLGPARWLALMQRWGEPEAVLAARQDDLAEILGPALA
                     RALMDTDATQPGRQAVHDWLAAGPLRQVLRLDDSDYPSGLLNTPCPPPLLFLLGRREL
                     LQRPMLAVVGSRHASVQGAQTAESWAEALGQAGLTIVSGMAAGIDAAAHTGALRTAAS
                     SVAVIGTGIDRIYPARNQALARQLAQTGLILSEFPLGAPPLAQHFPRRNRLIAGLSQG
                     CLVVEASLKSGSLITGRLAAEYGRDVFAVPGSIHSPQAKGCHRLIRDGAKLVDCLDDI
                     LQELRLPAAPVQSPSPTPASPPADETESRLLEAMGFDPVDADILCQRAGLTADVLSAM
                     LLRLEMAGRLETLPGGRYQRTA"
     gene            6459..6914
                     /locus_tag="PKN55_06770"
     CDS             6459..6914
                     /locus_tag="PKN55_06770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008954816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 domain-containing protein"
                     /protein_id="HNX70979.1"
                     /translation="MFDVFAYLVQEYPDFSACPDAEQLARRLFAAGFEDEDIEDALSW
                     LDLLDSAAAPEPLAQPAGGMRVFNDEEMDRLSLETRGFLQFLDANQVLDMSQRELVIE
                     RLLELPPSEVDSDVARLVVLMVLWRQCITPDILMAEELLRAIGWSSTLQ"
     gene            7024..>9314
                     /gene="topA"
                     /locus_tag="PKN55_06775"
     CDS             7024..>9314
                     /gene="topA"
                     /locus_tag="PKN55_06775"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HNX70980.1"
                     /translation="MPANLLIVESPSKAKTLKKYLGSDFEILASYGHIRDLVPKNGAV
                     DPDKNFAMKYQIIAKNSKHVDAIAAAVREAQHIYLATDPDREGEAISWHLLEILKNKK
                     LLKDKTAQRVVFHEITKQAVLDAVAHPRDISQELVNAQQARRALDYLVGFNLSPLLWK
                     KIRRGLSAGRVQSPALRLICEREAEIRAFVSQEYWSIHLDSHKGRTKFSARLTHYQGN
                     KLDQFDIPDDAAQAAILAALNGQSAQVSAVEKKKKSRSPAAPFTTSTLQQEAVRKLGM
                     TTDRAMRAAQQLYEGVDVGSGGTIGLITYMRTDSVALANEAVTEIRHYIDKHFDADYL
                     PKAPVTFKNKAKNAQEAHEAIRPTSIYRTPEALKPFLTAEQFKLYDMIWKRTLACQMT
                     PAKFDTTSVDIAVGGALSTFRASGQILVFPGFIAVYQEGEDDVVDEGEAKLPPLEVGD
                     ALPVDKLYGEQHFTQAPPRFSEASLVKALEEFGIGRPSTYASIIKTLKDREYVNLDKK
                     RFIPTDTGDIVNKFLTEHFQQYVDYNFTAKLEDQLDEISTGKREWVPVLDGFWKGFHK
                     QLEEKEGISRAEVTTEALDEACPKCGKPLNIRFGKRGRFIGCTGYPECDYTRNLNDDP
                     NAPQEAPVVIEDRVCPEDGGQLVVKNGPYGKFIGCANYPKCKHIEPLEKPKDTGVECP
                     ECKAGHLIERKSRYGKLFYSCNTYPKCKYATWNPPVAEPCPKCGWPILTLKTTKRRGT
                     EKVCPQKECGYSEVVIPPEPKPG"
BASE COUNT         1935 a         2961 c         2788 g         1530 t
ORIGIN      
        1 ccacgcacgc gccggcaatg atcagcaccg ggttgttcag ggtaaagccg atgccggcag
       61 ccgcccagcc cgagtaggag ttcagcatcg acaccaccac cggcatgtcg gcgccgccaa
      121 tcgggatgat cagcgtcacg cccagcacca gggcgatggc caccatgatc aggaaggcgg
      181 cgtggctgtc ggtgaagaaa tacaccccgc caaagcccac catggccagc gccatgacca
      241 ggttcagcag atgctggccg ttgaagttca cggccttggc gccgaacttg ccggacaatt
      301 tgccgtaggc aatcaccgag gcggtgaagg tgatggcgcc gatgaaggcg ccgatgaaca
      361 gctcgacctt ttgcaccggg gtgtgttcca tgccgctatg gaagatagca gccacggcaa
      421 tcagcacagc cgacaggccc accagcgagt gcatggcggc caccagctcg ggcatgctgg
      481 tcatttccac ggttttggct ttgaacgcgc caatcgcggc gccggcggca atcgcgccga
      541 caatcagcaa cagcacgggt ttttccatca ccatgaaggt ggtggccacg gcgatcagca
      601 tgccgatcat gccgtaggtg ttgcccttgc gcgagctggc cggcgaggac aggcccttga
      661 gcgacaggat gaacagcacc gccgacacca ggtacagcac agcagaaata ttttgcatgg
      721 cgggctcctt atttcttctt cttgaacatg tcgagcatgc gctgggtgac caggaagccg
      781 ccgaagatgt tgatgctggc caggaagatg cccacggcgc ccagcaccga ggtgacggtc
      841 agcgtctggc cgttgatgtc caccacttgc agcatggcgc cgacgatgat gatgccggaa
      901 atggcgttgg tcaccgccat cagcggggta tgcagggccg gagtgacgtt ccagaccacg
      961 tggtaaccga cgaagaccgc cagcacaaag atggtcaggc tgatgatgaa tggatcagtc
     1021 atgatgattg gtttcctttt gccggtgact caggcggcct ggccaaagcg cagcacgccc
     1081 tgatgggtga tcagggtggc ggccagcagg tcgtcttcca ggttcagcga gaagccttcc
     1141 ttggtcagca tcaggctgag gaaggtcagc aggttcttgg cgtacaggct ggaggcgtcc
     1201 gccgccacca tgcccggcag attggtgaag cccaccacgg tgacgccgcc gtcagtggtg
     1261 aaggccgcac cggggcgggt cagcggacag ttgccgcccg actctgccgc gatatccaca
     1321 atcaccgcgc ccggcttcat gccggccacc acatcggctt ccagcaggat cggggcttgt
     1381 ttgcccggaa tcagcgccgt ggtgatgacg atgtcagccg ccttggcgtg cttggccacc
     1441 aactcgccct gacgacgctt gtagtcgtcg gacatttctt tggcgtagac gccatcgttg
     1501 gcttttttct cttcgtcggt catcggcact tcaacaaact tgccgcccag cgactcgact
     1561 tgctccttgg tggccggacg cacgtcaaac gcctcgacca cggcgcccag gcgcttggcg
     1621 gtggcgatgg cctgcaaacc ggccacgcca gcgcccagca ccagcacctt ggcggctttc
     1681 accgtgccgg cggcggtcat cagcatcggc atgaagcgcg ggtagaactg ggtggccagc
     1741 agcacggcgc ggtagccggc gatgttgttc tggctggaca gcacgtccat gctctgcgcg
     1801 cgggtggtgc gcggcagcag ctccatggtg aaggcgctga tctggcggct ggccagctcg
     1861 gccagcgtgt tgttgcggtg cggatcgagc atgccgatca gcacggcgcc ggctttcagc
     1921 gagggaatat cctcggcagg cagcgcgcgc acagtcagca cgatgtcggc agcggccagc
     1981 agcgcggcgc ggtcgcccac cacggtggcg ccagcggcga tataggcgtc gtccagcacg
     2041 gcggaagcgc ggccagcgcc cgattgcacg gcgacggcgt gaccgagggc gacgtatttc
     2101 ttcaccgtgt ccggtgtcgc cgccacccgg gtttcaccgg ccgccagttc ggcgggaatg
     2161 gcaatttgca tgtgaataga cctcacggag ttgacaaatg gtttgacaat gtcatgccag
     2221 gcgtgaggtg acgaaggcgt aaggggtaaa cccttgcccg tgatgagggg ccggccgcgc
     2281 tcgtcagaag ctctggcgtg acgggtcgga aaggcgtcgt caatcggcgg gcagcagcaa
     2341 cctgtcgact cacggcgcat ttcaaacgca cgtttaattt gtatggctat ttaaaccatg
     2401 aaacgtcctt tttgtgaagt atttttacgt atatgcgacg ttataaacca acaccttgat
     2461 gcgttgcaat aattttgtgc atttcaccct gctaggtcag accaaatgac caaaagcgct
     2521 atctccatga atacacagat acaattcgat acatttgtgt aacgcttgcc gtgacaggac
     2581 aaaatgtccc gcccgcaagg cggaacgaga cattttgcag cgcacaaatc aacagcgaac
     2641 gaacatggcc gctgaacggt gtggcaactt gtctacagcg catcaatggc ggcgcttagc
     2701 cgctttacct tccccgtaaa gcgcgacatc tcaccccagc acttcccagg caaaccgccc
     2761 aatcagcacc gtcagcaaca ccagaaacag cacacgcacc acctggctgc cgccgcgcag
     2821 cgccagccag ctgccgctga tcgcccccag cacattgcac gccgccatcg gcagggcgta
     2881 atgccacaac acctgatcct gcggcaaaaa gtaactcagc gcggcgatat tggtggcgat
     2941 gttgacaaat ttggccgccg ccgacgcatg caaaaaatca aaggcaaaac agcgaataaa
     3001 caaaaaaatc aaaaagctgc cggtgccggg gccaaacacg ccatcgtaaa aaccaatcgc
     3061 cccgcccagc gcggtggcca tcacccgctc gcgccggccg atgctgacgg gcgcatgcag
     3121 cgcgccaaaa tcttttttcc acagcgtgta caccgccatc agcagcaaca agaccagcac
     3181 caaggggcgc atcaccgact tggggatcca gctcaccaca ctggcgccaa caaaagaaaa
     3241 cgcaaacgcc gccagcgctg ctggcaacac cagcttccac ggcaggcgca cctggcgcac
     3301 ataactgcgc gcggcaaaca gcgtgccgga caccgccgcc accttattgg tgccaaacag
     3361 cgtggccggc gccacatggg gcagggcgtt gaacagcgca ggaatctgga tcaaaccgcc
     3421 gccacccact gccgcatcaa tcaaaccggc catgaacgcc atcaggccca gaaacggcaa
     3481 agactcactc attgacatca cacgactgca ttaaaaacgc agcctaacat agctgttgct
     3541 gacggtgaaa cacccaaaaa gcaactatat tgtcataatt tgtgggcatg tgccggcaaa
     3601 accggcgaag tgctggcgca aaagcttgct ggatgggcat ttgggcaaag ttgtcatatg
     3661 gcgcaatcgt cagacgccgc gcagcggggc atactaaagt tgcgtgcgcc agacgctgcc
     3721 tgcgcggcag cccaatggca gtaatttatc gacaaattat cgataaattg ccatgataaa
     3781 caccgctccc ccacttcagc gccgctcacc aaacttagcg cagtcatcct ggctaggcgc
     3841 acgaccgcag atagtacaat cagtacggca aggtcgcgcc actccgccag gcgggttttg
     3901 tgagcggcgc ttaacccgcc ccgacgccgc ccgcgctcgc gggcgtcacg gaagacacga
     3961 catggacatt caagcgtata tgctcgacct cggccgcgcc gcccgcgccg ccagccgcca
     4021 acttgcccgc accgacaccg cgcaaaaaaa ccgcgccttg ttggcgattg ccgacgcgct
     4081 gcaacgcgac agtgatgcac tggtgaccgc caatcaggct gatctggccg ccgcccgcga
     4141 cgccgggctg gagccggcca tgctcgaccg gctgacgctg tcggcggcgg gcgtggcgca
     4201 aatggccgaa ggcctgcgcc agattgccgc cctgcccgac ccggtgggcg aaatgggcga
     4261 atttgcctac cgcccgtccg gcatccagct gggcaagatg cgcgtgccgc tgggcgtgat
     4321 tggcatcatt tacgaagccc gccccaatgt gaccgccgac gccgccggcc tgtgcctgaa
     4381 atccggcaac gcctgcattt tgcgcggcgg ctcggaagcc atccgcagca accaggccat
     4441 cgccgcctgc gtgcacgaag gcctgcgcga ggccggcctg ccggaaggcg cagtgcaggt
     4501 gatcaacacc accgaccgcg ccaccgtcgg cgcgctggtg accatgaccg agtttgtcga
     4561 tgtgattgtg ccgcgcggcg gcaagggcct gattgcccgc gtgtcggccg aggcgcgcgt
     4621 gccggtgatc aagcacctgg acggcaactg tcatgtgtat gtggacgtgg acgccgacct
     4681 ggtgaaggcc gggcgcattg ccgacaacgc caaaacccag cgctacggca cctgcaacac
     4741 catggaaacc ctgctgaccc accacaccgt ggcggccgaa ttcctgccgg tgattgccgc
     4801 catctacctg agcaagggcg tggagctgcg cggctgcccg cgcgcgcaag agatcatcgg
     4861 cgcccaggtc aaaccggcca gcgaagacga ttggtttacc gaatacgccg cgccgattgt
     4921 ggcgctgaaa gtggtggaca gcctggacga ggccatcgag catatcaacc actacggcag
     4981 ccaccacacc gacgccatcg tcaccgaaaa ctacaccacc gcccgccgat tcttgcgcga
     5041 ggtggattcg tccagcgtga tggtcaacgc cagcacccgc tttgctgatg gctttgaata
     5101 cggtctgggc gccgaaatcg gcatttccac cgacaaaatc cacgcccgtg gcccggtggg
     5161 cctgcatggg ctgacnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnntggct
     5281 gcactgcctg ctgaccgatg gactggggcc ggcgcgctgg ctggccttga tgcaacgctg
     5341 gggcgagccc gaggcggtgc tggcggcgcg gcaggatgat ctggcggaaa tcctcggccc
     5401 ggcgctggcg cgtgccctga tggataccga tgccacccag cccggccggc aggcggtgca
     5461 cgactggctg gcggccgggc cattgcggca agtgttgcgg ctggacgaca gtgattaccc
     5521 ctcgggctta ttgaacaccc cgtgtccgcc gccgctgttg tttttgctgg ggcggcgcga
     5581 attattgcaa cggccgatgc tggccgtggt gggcagccgc cacgccagtg tgcagggggc
     5641 gcaaaccgca gaaagctggg ccgaggcgct ggggcaggcc gggctgacca ttgtcagcgg
     5701 catggccgcc ggcatcgacg ccgccgccca caccggcgcc ttgcgcacgg cggcatccag
     5761 cgtggcggtg atcggcaccg gcattgaccg gatttacccg gcgcgcaatc aggcgctggc
     5821 gcgtcagttg gcgcaaaccg gcctgatcct gtcggaattt ccgctgggtg cgccgccgct
     5881 ggcgcagcat tttccgcgcc gcaatcgcct gatagccggg ctcagtcagg ggtgtctggt
     5941 ggtggaagcc agcctgaaaa gtggctcgct gattaccggc cggctggcgg cggaatatgg
     6001 ccgcgatgtg tttgccgtgc cggggtcgat tcattcgccg caggccaagg gctgccaccg
     6061 tttgatccgc gatggcgcca aactggtgga ctgtctggat gatattttgc aagaactgcg
     6121 cctgcctgcc gcccccgtcc aatccccttc accgaccccg gccagcccgc cggccgacga
     6181 gacagaaagc cgtttgctgg aggcgatggg ctttgacccg gtggatgctg acatcttgtg
     6241 tcagcgagcg ggcttgacgg ccgatgtgct ctcggccatg cttttgcgcc ttgaaatggc
     6301 cggtcggctg gaaaccctgc ccggcggccg ctatcaacgc acggcctgag caccaggctt
     6361 ccatccgcac atatccgcgt cttgacagcc gtggtaacgt atataaagca cgcgccaagc
     6421 ccggcgcgcc gcccagaacc acacggtaag gtttttcaat gttcgatgtt ttcgcctatc
     6481 ttgttcagga gtatccggac ttcagcgcct gtccggacgc cgagcaactg gcgcgccgcc
     6541 tgtttgccgc cggcttcgag gatgaagata tcgaagacgc gctcagctgg ctggatttgc
     6601 tggacagcgc cgccgcgccc gagccgctgg cgcagccggc gggcggcatg cgggtgttca
     6661 acgacgaaga aatggaccgc ctgtcgctgg aaacccgagg ctttttgcag tttctcgacg
     6721 ccaatcaggt gctggacatg agccagcgcg agctggtgat cgaacgtctg ctggaattgc
     6781 cccccagcga agtggactcg gatgtggctc gcctggtggt gttgatggtg ctgtggcgac
     6841 aatgcattac cccggatatc ctgatggccg aagagctgct gcgcgccatc ggctggtctt
     6901 ccaccctgca atagccgggt tgggactggc atcgcggccg cgtcagacgc ggccttgtta
     6961 tctctattca aaggaatggc cgccggcctg atggcgcggc ctgcgattgc ctacggatct
     7021 gccatgcctg ccaatctatt gatcgtcgaa tcgccgtcca aggcgaagac cctgaaaaaa
     7081 tacctgggca gcgattttga aattctcgct tcctatggcc acatccgcga cctggtgccg
     7141 aaaaacggcg ccgtggatcc ggacaaaaac tttgcgatga aataccagat catcgccaag
     7201 aacagcaaac acgtggacgc catcgccgcc gccgtgcgcg aggcccagca catctatctg
     7261 gcaactgacc cggaccgcga aggtgaagcg atttcctggc atttgctgga aatcctgaaa
     7321 aacaaaaagc tgctgaaaga caaaaccgcc cagcgcgtgg tgtttcacga aatcaccaag
     7381 caagccgtgc tggacgccgt ggcccacccg cgcgacatct cgcaagagct ggtcaacgcc
     7441 cagcaggcgc gccgcgcgct ggattatctg gtgggcttta acctgtcgcc gctgctgtgg
     7501 aaaaaaatcc gccgtggcct gtccgccggc cgcgtgcaaa gcccggcgct gcggctgatc
     7561 tgcgagcgcg aagccgaaat ccgcgccttt gtctcgcagg aatactggtc catccacctg
     7621 gacagccaca aaggccgcac caagttctcc gcccggctga cccactacca gggcaataaa
     7681 ctcgaccagt tcgacatccc cgacgacgcc gcccaggccg ccatcctggc cgcgctgaac
     7741 ggccagtcgg cgcaagtcag cgccgtcgaa aagaaaaaga aatcgcgcag cccggccgcg
     7801 ccgttcacca cctccaccct gcaacaggag gccgtgcgca aactgggcat gaccaccgac
     7861 cgcgccatgc gcgccgccca gcagctgtac gaaggcgtgg acgtcggcag cggcggcacc
     7921 atcggcctga tcacctatat gcggaccgac tcggtggcgc tggccaacga ggcggtgacc
     7981 gaaatccgtc attacatcga caagcacttt gacgccgact acctgcccaa agcgccagtc
     8041 accttcaaga acaaggccaa aaacgcccag gaagcgcacg aagccatccg cccgacctcg
     8101 atctaccgca cgccagaggc gctcaagccc ttcctgaccg ccgagcagtt caagctgtac
     8161 gacatgatct ggaagcgcac cctggcctgt cagatgaccc cggccaagtt cgacaccacc
     8221 agcgtggaca tcgccgtcgg cggcgcgctg agcaccttcc gcgccagcgg ccagattctg
     8281 gtgttccccg gctttatcgc cgtgtaccag gaaggcgaag acgatgtggt ggacgagggc
     8341 gaagccaagc tgccgccgct ggaagtgggc gacgccctgc cagtggacaa gctgtatggc
     8401 gagcagcact tcacccaggc gccgccgcgc ttctcggaag ccagcctggt gaaggcgctg
     8461 gaagaattcg gcatcggccg gccgtcgact tacgcctcga tcatcaagac gctgaaagac
     8521 cgcgaatacg tcaacctgga taaaaaacgc tttatcccca ccgacaccgg cgacatcgtc
     8581 aataaattcc tgaccgagca ttttcagcag tacgtcgatt acaacttcac cgccaagctg
     8641 gaagaccagc tcgatgaaat ctccaccggc aagcgcgagt gggtgccggt gctggacggc
     8701 ttctggaagg gctttcacaa gcagctggaa gaaaaagaag gcatcagccg cgccgaagtc
     8761 accaccgaag cgctggacga agcctgcccc aagtgcggca agccgctgaa catccgcttt
     8821 ggcaaacgcg gccgctttat cggctgcacc ggctacccgg aatgcgacta cacccgcaac
     8881 ctcaacgatg accccaacgc cccgcaagaa gcgcccgtgg tgatcgaaga ccgcgtgtgc
     8941 ccggaagacg gcggccagct ggtggtgaaa aacggcccgt atggcaagtt catcggctgc
     9001 gccaactacc ccaagtgcaa acacatcgag ccgctggaaa aacccaagga caccggcgtg
     9061 gaatgcccgg agtgcaaggc cggccacctg atcgaacgca agagccgcta cggtaagttg
     9121 ttctacagct gcaacaccta ccccaagtgc aagtacgcca cctggaaccc gccagtggcc
     9181 gaaccctgcc cgaaatgcgg ctggccgatc ctgacgctga aaaccaccaa gcggcgcggc
     9241 accgagaagg tctgcccgca aaaagaatgc ggctacagcg aagtggtgat tccgccggaa
     9301 ccgaaaccgg gcaa
//
LOCUS       DAOPYX010000102         9299 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_8750_length_9299_cov_8.403721, whole genome shotgun sequence.
ACCESSION   DAOPYX010000102 DAOPYX010000000
VERSION     DAOPYX010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 9299)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9299)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9299
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8750_length_9299_cov_8.403721"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..285)
                     /locus_tag="PKN55_06780"
     CDS             complement(<1..285)
                     /locus_tag="PKN55_06780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011135750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HNX70981.1"
                     /translation="MTPQGLTILGATGTIGVNTLDVVSRHPQQFRVVALTGQSQLDKL
                     AEQCRRFAPRYAVVLDAASASTLSGLLAAAGCDTEVLYGAEALAQVATLPE"
     gene            complement(296..1102)
                     /locus_tag="PKN55_06785"
     CDS             complement(296..1102)
                     /locus_tag="PKN55_06785"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014087623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HNX70982.1"
                     /translation="MLIPRIITAVFLVAFMLGGLFYLPAPYWPAFAAVIILPALWEWT
                     RLFHITGLAQVGYLGFSALMMYAVASTGQMPGAGMQVLSLLMWLVVAPLWLWRRWKLV
                     DRNQAAMLGWLLLMPAWFALLAWRPGPEQGPALLAVMALVWIADTAAYFAGRAFGKHK
                     LAPAISPGKSWEGVAGGMLATGIYTLWVGHSGVFDFSLPWWGWLAAGWALTAVSVVGD
                     LLESWFKRAAGVKDSSQLLPGHGGVLDRIDSLIAVLAVSQAARYLATQWM"
     gene            complement(1149..1910)
                     /locus_tag="PKN55_06790"
     CDS             complement(1149..1910)
                     /locus_tag="PKN55_06790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014087624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HNX70983.1"
                     /translation="MFLSSTQTIPDVASVPRHIAIIMDGNGRWAKKRFLPRVAGHKRG
                     VEAVRNTVKSCAEMGVEYLTLFAFSSENWRRPQDEVSFLMELFLQVLEREVERLHRNN
                     IRLKIIGASERFNPALLERIARAEEKTAANSGLTLTVAADYGGRWDVLQAVKRLAVER
                     HGDLSGVSEADLAGQLSMAYAPEPDLFIRTGGEQRISNFLLWQLAYTELHFTQTLWPD
                     FDRAALDLAIDSYRQRERRFGRTTEQLDPALRRAE"
     gene            complement(1985..2542)
                     /gene="frr"
                     /locus_tag="PKN55_06795"
     CDS             complement(1985..2542)
                     /gene="frr"
                     /locus_tag="PKN55_06795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HNX70984.1"
                     /translation="MIQDVKKQAESKMQKTLDAFKADLTKVRTGRAHTGLLDHVMVDY
                     YGNPTPVNQVGNVTLIDARTIGVQVWEKNMVAKVEKAIRDSDLGLNPAANGDVIRVPM
                     PALTEERRRDLTKVVKGEAENARVAVRNIRRDANNDCKNLLKDKSITEDEERRAQDDI
                     QKLTDKYIVEVDKALAEKEKELMAV"
     gene            complement(2645..3370)
                     /gene="pyrH"
                     /locus_tag="PKN55_06800"
     CDS             complement(2645..3370)
                     /gene="pyrH"
                     /locus_tag="PKN55_06800"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008953643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HNX70985.1"
                     /translation="MSQTPTPMYRRILVKLSGEALMGEDSYGINRKVIEQIVGEIKQV
                     AELGVQIGVVIGGGNIFRGIAPAASGMDRATADYMGMLATVMNALALKDAMSKAGLVA
                     RVQSALAMQQVAEPYVRGKAMQYLEEGKIVIFGAGTGNPFFTTDTAAALRGIEMGADV
                     MLKATKVDGVYTDDPKKNPDAVRYQSLTFDEAIARNLKVMDATAFALCRDQQFDLKVF
                     SIFKQGALKRVVLGEDEGTLVHC"
     gene            complement(3583..4470)
                     /gene="tsf"
                     /locus_tag="PKN55_06805"
     CDS             complement(3583..4470)
                     /gene="tsf"
                     /locus_tag="PKN55_06805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012697543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HNX70986.1"
                     /translation="MAEITAKMVADLRAATGLGMMECKKALVETEGDVKAAEELLRIK
                     SGAKASKLAGRTAAEGVVAQFISADGKVGALVEVNCETDFVGKDEGFVAFAKAVAQAV
                     VTGNPADVEALAAVATAEGPTVEETRKALIAKLGENMSVRRFVRYETAGKVAAYLHGA
                     KIGVLVDLAGGEEQVGKDVAMHIAASKPICVSKEQVPAENLETERRIYTEQAAQSGKP
                     ADIVAKMVEGRINKYLAEVTLLGQQFVKNPDITVEKLLKDNQAAVNAFTMFVVGEGIE
                     KKVEDYAAEVAAAAAAIKG"
     gene            complement(4588..5328)
                     /gene="rpsB"
                     /locus_tag="PKN55_06810"
     CDS             complement(4588..5328)
                     /gene="rpsB"
                     /locus_tag="PKN55_06810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008953641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HNX70987.1"
                     /translation="MTTAVTMRQMLEAGVHFGHQTRFWNPKMNQYIFGSRNKIHIINL
                     EKTLPLFLEAQTYIRRLAANKGTVLFVGTKRQAREIVREEASRAGVPFVDHRWLGGML
                     TNYKTVKQSIKRLEDKMALLASGEESGYSKKELLDLQREVEKLERSLGGIKDMKGLPD
                     AIFVIDTGYQKGAVVEAKKLGIPVIGVVDTNNSPEGIDYVIPGNDDSSRAIRLYARGI
                     ADAVLEGRAQAIQEIVAAAAAAEPVAAE"
     gene            complement(5509..5700)
                     /locus_tag="PKN55_06815"
     CDS             complement(5509..5700)
                     /locus_tag="PKN55_06815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX70988.1"
                     /translation="MYVVLMFALGQRTLPMTIFVLILFGALPTWLLLFVVRRRQAMKA
                     NGDIAPRKPRRPRGGQSED"
     gene            complement(5783..6280)
                     /locus_tag="PKN55_06820"
     CDS             complement(5783..6280)
                     /locus_tag="PKN55_06820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011135917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HNX70989.1"
                     /translation="MTDTHTLAAQLGEALRQRGQRIATAESCTGGLIAGALTAIAGSS
                     DWFGFGWVSYANEAKQQMLDVRAETLMAHGAVSGQTVREMAEGARRHSGADWAIAVSG
                     IAGPGGGSRDKPVGLVWFGLAGPDGVKAFSEVFAGDRDAVRQHTVNTALTQLLARVNA
                     STLIA"
     gene            complement(6277..7932)
                     /gene="pgi"
                     /locus_tag="PKN55_06825"
     CDS             complement(6277..7932)
                     /gene="pgi"
                     /locus_tag="PKN55_06825"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011135916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HNX70990.1"
                     /translation="MTQPRHYDLNHTSIWAKLHQHQRATRHMHMRELFAADPQRFQRF
                     SLSLGPLLMDYSKHRITERTLELLFELADAAHLKDWRQRMRAGEAINVSEKRAVLHMA
                     LRGQAGDDFQVDGQPVMAQVLAVRARMAAFAEQVRSGAWLGFDGRPIRAVVNLGIGGS
                     DLGPLLACRALRPYAAAQLDMRFVANLDGQHLAQALQGLEPATTLFIVASKSFTTPET
                     LMNAEAARAWLLAAAPAEAVARHFVAISSQTERVRAFGIDPANMFEFWDWVGGRYSVW
                     SAIGLSVMLSIGPAAFEDFLAGARRMDAHFLEAPFAQNIPVILALLGVWYNTFYRAHT
                     HAIMPYDQGLARLPAYLQQLDMESNGKRVGRYGETLDFDTGPVIWGEVGVNSQHAFFQ
                     LLHQGTRLVPCDFIVPMRSHYPLDRHHEALVANCFAQTEALMRGKTEAEVLGELAGLD
                     GDTLDMLLPQKLFPGNQPSTTLALDAITPHTLGMLLAMYEHKVFVQGVIWGINSFDQW
                     GVEYGKQLARQIQPELADASAALTHDASTNALIAHYRARKAQP"
     gene            complement(8177..8566)
                     /gene="acpS"
                     /locus_tag="PKN55_06830"
     CDS             complement(8177..8566)
                     /gene="acpS"
                     /locus_tag="PKN55_06830"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012696944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HNX70991.1"
                     /translation="MILGIGTDLVDIARMRAALDRHGERFARRILSPAEWPDWQASGD
                     PARLLAKRFAAKEAFAKASGQGLRAPVTLGALAVTHDALGRPSWAFADELAQWLTARG
                     VQRCHLSISDERAQCLAFVVLEGGQID"
     gene            complement(8563..9294)
                     /gene="pdxJ"
                     /locus_tag="PKN55_06835"
     CDS             complement(8563..9294)
                     /gene="pdxJ"
                     /locus_tag="PKN55_06835"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008953855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HNX70992.1"
                     /translation="MILLGVNIDHVATLRNARGVNYPSPLEAALVAETAGADLITLHL
                     REDRRHIKDEDVRLMRRALKTRMNLEMALTPEMLDHALAIRPDDVCLVPEKREELTTE
                     GGLDVERYFDQVRDYTRQLSAAGIRVSLFIDPTETSLSAAVEAGAPVVELHTGRYADA
                     IGQAARQSELARIRDAAEIGVYKGLVVNAGHGLNYHNVQPVAAIAEIRELNIGHAIVA
                     QALFTGFDRAVRDMKALMVEARRTA"
BASE COUNT         1603 a         2916 c         3003 g         1777 t
ORIGIN      
        1 ttccggcagg gtggccacct gcgccagcgc ctcggcgccg tacagcactt cggtgtcgca
       61 gccggcggcg gccagcaggc cggacagcgt gctggccgag gcggcgtcca gcaccacggc
      121 gtagcggggg gcaaagcggc ggcattgctc ggccagcttg tccagttgcg attggccggt
      181 gagcgccacc acgcgaaatt gctgtgggtg gcggctcacc acgtcgaggg tgttgacgcc
      241 gatggtgccg gtggcgccca gaatagtcag gccttgcggg gtcatgttca gcagcttaca
      301 tccattgggt ggcaaggtaa cgggcagcct ggctgaccgc cagcacggcg atcaggctgt
      361 cgatgcggtc gagcacgccg ccgtggccgg gcaagagctg gctgctgtct ttgacgccag
      421 cggcgcgctt gaaccaggat tccagcaggt cgcccaccac gctgaccgcg gtgagcgccc
      481 agccagcggc cagccagccc caccacggca aagaaaaatc aaatacgccg ctgtgaccca
      541 cccacagtgt ataaattccg gtggccagca tgccgccggc cacaccttcc cagcttttgc
      601 cgggactgat ggccggggcg agtttgtgtt tgccaaaggc gcgaccggca aagtaggcgg
      661 cggtgtcggc aatccacacc agcgccatca ccgccagcag ggccgggcct tgttccgggc
      721 cgggccgcca ggccagcagg gcaaaccagg ccggcatcag cagcagccag cccagcatgg
      781 cggcctggtt gcggtccacc agcttccagc gccgccacag ccacagcggg gcgaccacca
      841 gccacatcag cagcgacagc acttgcatgc cggcgccagg catctggccg gtgctggcca
      901 cggcgtacat catcagggca gaaaagccca gatagccgac ttgcgccagg ccagtaatat
      961 gaaacaagcg ggtccattcc cacagcgcgg gcaggatgat cacggcggca aacgccggcc
     1021 agtagggggc gggcaggtag aacagcccgc cgagcatgaa agccaccaga aatacggcag
     1081 tgatgatgcg gggaatcagc atggtcgggc cttgcagagc gacaccggca caggcgccgg
     1141 cgaatgggtc attccgcccg gcgcaaggcc gggtcgagtt gttcggtggt gcggccaaag
     1201 cggcgttcgc gctggcgata ggagtcaatc gccagatcca gcgcggcgcg gtcgaaatcc
     1261 ggccacagcg tttgggtgaa gtgcagctcg gtgtaggcca gttgccagag caggaagttg
     1321 ctgatgcgtt gctcgccgcc ggtgcggatg aacaaatccg gctcgggggc gtaggccatg
     1381 gacagttgcc cggccaaatc cgcctcgctg acgccagaca aatcgccatg gcgttcaaca
     1441 gccagacgct tgacggcttg cagcacgtcc cagcgcccgc cgtaatcggc ggcgacggtc
     1501 agcgtcaggc cgctgttggc ggcggttttt tcttccgcgc gggcaatgcg ctccagcagg
     1561 gcggggttga agcgctcgct ggcgccgatg attttcaggc ggatattgtt gcggtgcagg
     1621 cgctcgacct cgcgctccag cacttgcagg aacagctcca tcaagaacga cacttcgtct
     1681 tgcggccgac gccagttctc gctggaaaaa gcaaacaggg tgaggtattc cacgcccatt
     1741 tcggcgcagg atttcaccgt gttgcgcacg gcttccacgc cgcgcttgtg gccagccaca
     1801 cgcggcaaaa agcgtttttt cgcccaacgg ccattgccgt ccatgatgat ggcaatgtgg
     1861 cgcggcacgg aggcgacgtc gggaatggtc tgcgtggagc tgagaaacaa ggcggcatcc
     1921 tgcgggtaaa aaagacggaa agcggcagcg cgatgagggc gcgccgccgc actgggccga
     1981 agggtcagac ggccatcagt tccttttctt tttcggccag ggccttgtcc acttcgacga
     2041 tgtatttgtc ggtcagcttc tggatgtcgt cctgggcgcg gcgctcttcg tcctcggtga
     2101 tggacttgtc tttcagcagg tttttgcagt cgttgttggc gtcgcggcgg atattgcgca
     2161 ccgccacgcg ggcgttttcg gcctcgccct tgaccacctt ggtcaggtcg cggcggcgtt
     2221 cttcggtcag cgccggcatc ggcacgcgga tcacgtcgcc attggcggcc gggttcaggc
     2281 ccaggtcgga atcacggatg gctttttcta ccttggcgac catgtttttt tcccagactt
     2341 gcacgccgat ggtgcgtgcg tcgatcaggg tgacgttgcc cacctgattc accggggtgg
     2401 ggttgccgta gtagtccacc atcacgtgat cgagcaggcc ggtgtgggcg cggccggtgc
     2461 gcaccttggt caggtcggcc ttgaaggcgt ccagggtttt ttgcatcttg ctttcggctt
     2521 gttttttcac gtcttggatc atggttgtca actccggtct gtctcggttc gcatggcgcg
     2581 ggcggacagc atgcgccgcc gcgccgattc aagctgacgg catgcgcgcc gtcgggcgat
     2641 aaactcagca gtgtacgaga gtgccctcgt cttcgccaag caccacgcgc ttgagcgcgc
     2701 cttgtttgaa gatgctgaac actttcaggt caaactgctg gtcacggcac agggcaaagg
     2761 cggtggcgtc catcaccttc agattgcggg caatcgcctc gtcaaaggtc aggctctggt
     2821 agcgcacggc gtccgggttt ttcttgggat cgtcggtgta cacgccatcc accttggtgg
     2881 ccttgagcat cacgtcggcg cccatttcga tgccgcgcag cgcagcggcg gtgtcggtgg
     2941 tgaagaaggg gttgccggtg cccgcgccaa aaatcacgat tttaccttct tccaggtatt
     3001 gcatggcctt gccgcgcaca tagggctcgg ccacttgctg catggccagc gccgattgca
     3061 cgcgcgccac caggccggcc ttgctcatgg cgtctttcag cgccagggcg ttcatcaccg
     3121 tggccagcat gcccatatag tcggcggtgg cgcggtccat gccggaggcg gccggggcga
     3181 tgccgcggaa gatattgccg ccgccaatca ccacgccgat ttgcacgccc agctcggcca
     3241 cttgcttgat ttcgccgacg atttgttcga tcactttacg gttgatgccg tagctgtctt
     3301 cgcccatcag ggcctcaccg gacagtttga caagaatgcg tcgatacatc ggggtcgggg
     3361 tctggctcat ggcgcttcct tctggatatt ctgggtgatt caatctgtat tcaatctggc
     3421 ttggccgggg agactgcgcc cgtggcaaaa aattccggca atctcgccgg ccaaacccac
     3481 tgtgcgcaaa gcacagttgc aaaaaagcac ccgcttgggg cggccggcgg atgccggcaa
     3541 ccctgcgcca gggtgctttc ctgttcagga gaagaactcc gctcagccct tgatggcggc
     3601 agcagcggca gccacttcag cggcgtagtc ttccactttc ttctcgatgc cttcgcccac
     3661 cacgaacatg gtgaaagcgt tgacagcggc ttggttgtct ttcagcagct tttccacggt
     3721 gatgtccggg ttcttgacga actgctgacc cagcagggtg acttcagcca ggtatttgtt
     3781 gatgcggcct tccaccatct tggcgacgat gtcagccggc ttgccggact gggcagcttg
     3841 ctcggtgtag atgcggcgtt cggtttccag gttttcggcc ggcacttgct ctttggaaac
     3901 gcagatcggc ttggaagcgg cgatgtgcat ggccacgtcc ttgcccactt gctcttcgcc
     3961 gccagccagg tcaaccagca cgccgatctt ggcgccgtgc aggtaagcgg ccactttgcc
     4021 ggcggtttcg taacgcacaa aacgacgcac ggacatgttt tcgcccagct tggcgatcag
     4081 cgccttgcgg gtttcttcca cggtcgggcc ttcagcggtg gccacggcgg ccagggcttc
     4141 gacgtcggcc gggttgccgg tcaccacggc ttgagcaaca gccttggcga aggccacaaa
     4201 gccttcatcc ttgcccacga agtcggtttc gcagttgact tcaaccaggg cgcccacttt
     4261 gccgtcggcg gagatgaact gagccaccac gccttcagcg gcggtgcggc cagccagctt
     4321 ggaggccttg gcgccggact tgatgcgcag cagctcttca gcagccttca cgtcgccttc
     4381 ggtttccacc agggcttttt tgcattccat catgcccagg ccagtggccg cgcgcaggtc
     4441 agccaccatc tttgcggtaa tttccgccat gtttcgctcc taagtgcttg aacgggacgc
     4501 gccgagtatg aggcgacccg atgacaatct atagcatgga tttgaaaaag ggggcttgcg
     4561 cccccttttc ggtcaggccg tcgagaatta ctcagcggca accggctcgg cagctgcggc
     4621 ggcagcgacg atttcctgga tagcctgagc acggccttcc agcacggcgt cggcgatgcc
     4681 acgggcgtac agacggatcg cgcggctgga gtcatcgtta cccgggatca cgtagtcgat
     4741 gccttccggc gagttgttgg tatccaccac gccgatgacc gggatgccca gcttcttggc
     4801 ttccaccacg gcgccctttt ggtagccggt gtcgatcacg aagatggcgt ccggcagacc
     4861 cttcatgtcc ttgatgccgc ccagcgaacg ctcgagcttt tccacttcgc gttgcaggtc
     4921 cagcagttct ttcttggagt agccgctttc ttcgccgctg gccagcagag ccatcttgtc
     4981 ttccaggcgc ttgatggatt gcttgaccgt cttgtagttg gtcagcatgc cacccagcca
     5041 gcggtggtca acaaacggca cgccagcgcg ggaagcttct tcgcgcacga tttcacgagc
     5101 ctgacgcttg gtgccgacga acagcacggt gcccttgttg gcggccagac gacggatgta
     5161 ggtctgggct tccaggaaca gcggcagggt cttttccagg ttgatgatgt ggatcttgtt
     5221 gcggctgccg aagatgtatt gattcatctt cgggttccag aaacgggttt ggtggccgaa
     5281 gtgaacgccg gcttccagca tttgacgcat ggtgactgca gtggtcatga aaactcctaa
     5341 agggttagac cgccattcac gcacagaatc ccggtcgaag cgggacaccc tttggcgcga
     5401 atgtgaggat tttaaatgtt tacccgccta ccaattttgg tgggtcagat aacttataag
     5461 tttagcacgg aatggcaaaa ttgctcaata gcctgtgtgg gcaagcgctc agtcctcgga
     5521 ctggccgccg cgcgggcggc gcggcttgcg cggggctatg tcgccattgg ccttcatcgc
     5581 ctgccgacga cgcacgacaa acagcaacag ccacgttgga agtgcgccga acagaatcag
     5641 gacgaatatc gtcatcggca gtgtgcgctg gcccagcgca aacatcagca ccacatacaa
     5701 ccagccaatc cagatgatat acattgttaa caccggccca ttgggcagca gtccaatcag
     5761 gccggcattg tagcagggca ggtcaggcga tcaaggtgct ggcgttgacc cgcgccagca
     5821 gttgcgtcag tgcggtgttc accgtgtgct ggcgcacggc gtcgcggtcg ccggcaaaca
     5881 cctcggaaaa cgccttcacc ccatccgggc cggccaggcc aaaccacacc aggcccactg
     5941 gcttgtcgcg gctgccgccg cccgggccgg caatgccgct gacggcgatg gcccagtcgg
     6001 cgccgctgtg ccggcgcgcg ccctcggcca tctctcgcac ggtttgcccg ctgaccgcgc
     6061 catgcgccat cagcgtttcg gcgcgcacgt ccagcatttg ctgcttggct tcgttggcgt
     6121 agctgaccca gccaaagcca aaccagtccg agctgccggc aatggcggtg agcgcgccgg
     6181 caatcagccc gccggtgcag gattctgcgg tggcgatgcg ctggccgcgc tggcgcagcg
     6241 cttcgcccaa ttgggcggcc agggtgtggg tgtcggtcat ggctgggcct ttctggcgcg
     6301 atagtgggca atcagggcgt tggtcgaggc gtcgtgcgtc agcgcggcgc tggcgtcggc
     6361 cagctcgggc tggatttgcc gggccagttg cttgccgtat tccacgcccc attggtcaaa
     6421 cgaattaatc ccccagatca ccccttgcac aaacaccttg tgttcgtaca tggccaacag
     6481 catgcccagg gtgtgtgggg tgatggcatc cagcgccagc gtggtggacg gctggttgcc
     6541 ggggaacagc ttttgcggca gcagcatgtc cagcgtgtcg ccgtccagcc cggccagctc
     6601 gcccagcacc tcggcttcgg tcttgccgcg catcagcgcc tcggtctggg caaagcagtt
     6661 ggccaccagc gcttcgtggt ggcggtccag cggataatgg ctgcgcatcg gcacaataaa
     6721 atcacacggc accagccggg tgccctgatg cagcagctga aaaaacgcat gctggctgtt
     6781 cacgcccacc tcgccccaga tcaccgggcc ggtgtcaaaa tccagcgttt cgccatagcg
     6841 ccccacccgc ttgccgttgg attccatgtc cagttgctgc aaatacgccg gcagccgcgc
     6901 caggccttgg tcgtagggca tgatggcgtg ggtgtgggcg cggtaaaagg tgttgtacca
     6961 cacgcccagc agggcaagaa tcaccgggat gttctgcgcg aatggcgctt cgagaaaatg
     7021 cgcgtccatg cgccgcgcgc cagccagaaa atcttcaaac gccgccgggc cgatggacag
     7081 catcaccgac aggccaatcg ccgaccacac cgaataacgg ccgcccaccc aatcccaaaa
     7141 ttcaaacata ttggccgggt cgatgccaaa ggcgcgcaca cgctcggtct ggctggaaat
     7201 cgccacaaag tgccgcgcca ccgcctcggc cggcgcggcg gccagcagcc aggcgcgcgc
     7261 cgcttcggcg ttcatcagcg tttctggcgt ggtgaacgat ttggaggcga caataaacaa
     7321 ggttgtcgcc ggctccagcc cttgcagcgc ctgcgccagg tgctggccgt ccagattggc
     7381 gacaaagcgc atgtccagct gcgccgccgc ataggggcgc agcgcgcggc aggccagcaa
     7441 gggccccagg tccgagccgc caatgcccag attaaccaca gcgcgaatcg gccggccgtc
     7501 aaagcccagc caggcgcccg agcgcacctg ctcggcaaac gccgccatgc gcgcgcgcac
     7561 cgccagcacc tgcgccatca ccggctggcc gtccacctga aaatcatcgc cagcctggcc
     7621 gcgcaaggcc atgtgcagca ccgcgcgttt ttcgctgaca ttgatcgcct cgcccgcgcg
     7681 catgcgctga cgccagtctt tcaagtgggc ggcgtcggcc agctcgaaca gcagctccag
     7741 cgtgcgctcg gtgatgcggt gtttggagta atccatcagc agcgggccca ggctgagcga
     7801 aaagcgctga aaacgctgcg ggtcggcggc aaacagctcg cgcatatgca tatgccgggt
     7861 ggcgcgctgg tgttggtgca gtttggccca gatggaagta tggttcaggt cgtagtgccg
     7921 gggctgggtc atgggtgtgg tcaaggcttt gggttgcttc gggtgcatca ggatgcggat
     7981 cggtctgccg gttgccggaa caacaggcgc agcagcacat taccctagca ttttttgccg
     8041 gcttaagcca tggcctgaat gagggtatgc cagcaaattc cgggcatggc ccaccttgct
     8101 gtttggtggg ccgtggcgag cttgtgccct cgttcccgct gcttgatggt gctgcgcgcc
     8161 gtctgagcag caaggctcag tcgatctgtc cgccttccag caccacaaag gccaggcatt
     8221 gcgcgcgctc gtcgctgatg ctcaggtggc agcgctgcac gccgcgcgcc gtcagccatt
     8281 gggccaattc atcggcaaat gcccagctcg gccggcccag cgcgtcgtgg gtgaccgcca
     8341 gcgcgcccag cgtcaccggc gcgcgcagac cctggccgct ggctttggca aaggcttctt
     8401 tggcggcaaa gcgcttggcc agcagccggg ccgggtcgcc gctggcttgc cagtccggcc
     8461 attcggccgg cgacaggatg cggcgggcaa agcgctcgcc gtggcggtcc agcgccgcgc
     8521 gcatgcgggc gatgtccacc aggtcggtgc cgatgcccag aatcatgccg tgcggcgcgc
     8581 ttcgaccatc agcgccttca tatcgcgcac ggcgcggtca aagccggtga acagcgcctg
     8641 ggcgacgatg gcgtggccga tgttcagctc gcggatttcg gcaatggccg ccaccggctg
     8701 cacattgtgg tagttcaggc cgtggccggc gttgaccacc agccccttgt acacgccgat
     8761 ttccgccgcg tcgcggatgc gcgccagctc gctttgccgg gcggcctggc cgatggcgtc
     8821 ggcgtaacgg ccggtgtgca gctccaccac cggcgcgccg gcttccacgg cggcgctgag
     8881 cgaggtttcc gtcgggtcga taaacagcga cacgcgaatg ccggcggcgc tgagctggcg
     8941 ggtgtagtcg cgcacttggt caaaatagcg ctcgacgtcc aggccgcctt cggtggtcag
     9001 ctcttcgcgc ttttctggca ccaggcagac gtcgtccggg cggatggcca gggcgtggtc
     9061 gagcatttcc ggggtgagcg ccatttccag gttcatgcgg gttttcagcg cgcggcgcat
     9121 caggcgcacg tcttcgtcct tgatgtggcg gcggtcttcg cgcagatgca gggtgatcag
     9181 gtcggcgccg gcggtttccg ccaccagggc ggcttccagc gggctggggt agttgacgcc
     9241 acgggcattg cgcagggtgg cgacgtggtc gatattgacg cccaacagaa tcatgatgt
//
LOCUS       DAOPYX010000103         9273 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_8778_length_9273_cov_12.431655, whole genome shotgun sequence.
ACCESSION   DAOPYX010000103 DAOPYX010000000
VERSION     DAOPYX010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 9273)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9273)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9273
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8778_length_9273_cov_12.431655"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(3..1271)
                     /locus_tag="PKN55_06840"
     CDS             complement(3..1271)
                     /locus_tag="PKN55_06840"
                     /inference="COORDINATES: protein motif:HMM:NF019350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HNX70993.1"
                     /translation="MDLRKRLTAWMGLFSLLLIACVSLYWLNAARADAEEELRASARL
                     LQLIDALGRADANPARVRELVAAGGFRHVTLRLGGGPDAPAGDEAPGWLAPLAAQVQF
                     DRQPIALNLDGHVVHVALEPYSEIEEKIRDASGVILFTVVISAISLVACWLTIGRALA
                     PARRIEAAIRALEDDSQPLTLPRFALREFDRIAQTLNTLSAKLHAARQRQRQLSRRLL
                     AVREQEQAELARELHDELGQSLEAIGVCAAYLLRHGPSEPEHPQRQCVRDIQQETQRI
                     HRHIRALLSELRPHGLDGLSLAEALEELTRQWQARRPEVQLDAHWQAMPPLPARHALQ
                     LYRAVQECLTNIARHSQATAVQLRAEVTDGCLCVSVSDNGVARVVPKYGCGLLGMRER
                     LESVGGGVTLALNPGGGLCVSLHAPLVGLS"
     gene            1561..4062
                     /locus_tag="PKN55_06845"
     CDS             1561..4062
                     /locus_tag="PKN55_06845"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNX70994.1"
                     /translation="MLQFYQIPCRGPARAPWLLCIAGLLGPAVAQAEDAALAEVTVVG
                     ATPLPGLGVSRAQVAAPVVSRNAAALQHSQRATLGEFLERELGSVHINDMQGNALQAD
                     VNYRGYTASPLLGTPQGLSVYLDGMRMNQPFGDVVSWDALPRSALATLTLMPGSNPLF
                     GLNTLGGALALTTKDGLSHPGTAIETGYGSDQRRRLGFEHGGSAANGLHWFVTGNWMK
                     ENGWREHSPSETRQLFAKLGWSGERTDLAAALGWADNTLTGNGLQDARLLARDYRSVY
                     TQPDETDNRSAWLNLTGKHQLNDRVQLSGNAYARRLSSSTLNGDINEDSLDQQVYLLR
                     TSTADRNALSKAGYSNLPSQNETAANTPFPYWRCLAQTALADEPAEKCNGLLNRTRTR
                     QTQYGFSGQFTLQDTGNQFTAGLGYDASRVRFSQTSQFGYLNPDRSVTPVNFFADGTE
                     IDDSGVPVDSRVNLSGRTRTWSLFATDTLTLAEHWHLTLSARWNQTRLSVRDHITPGG
                     GAGSMDGDHRFSRVNPAAGLSYAPSANLTAWLGYNQGSRAPSVVELGCADPSNPCRLP
                     NAMASDPALRQVVTHTWEAGVRGKFTPNLRWQLGGFRADSRDDILFVASRQSGSGYFK
                     NVDRTRRQGVELGLSGEHGAFNWGLDYTYLDATYQSAETLTAAANSSQDASGNIRVQP
                     GDRLPLTPRHLVKLRADYRFSPQWQAGVAMRAVAGSLARGNENGQHQSDGVYNLGPGR
                     SAGYAVWDLTGEYRPTTQLTVFGQVNNVFDRRYATAAQLGATGLTSAGNFVARPFAAV
                     GGKYPLMHATFYAPGAPRSFWLGVRYEFDKVAQKD"
     gene            4362..6218
                     /locus_tag="PKN55_06850"
     CDS             4362..6218
                     /locus_tag="PKN55_06850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019924931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol/ethanol family PQQ-dependent
                     dehydrogenase"
                     /protein_id="HNX70995.1"
                     /translation="MHTTIKHTLRRAPLAAVISLALAGAALAGTVSWEDIANDDKTPK
                     DVLMYGMGLKGQRFSALKQVSAKNVADLVPAWSFSFGGEKQRGQETQAIVHDGVIYIT
                     ASYSRMFAIDAKTGKQLWAYEHRLADDIRPCCDVINRGAAIYGDKVYFGTLDASIVAL
                     NKDTGKVVWKEKFGDHKIGYTMTGAPTVVKDQKTGRMLLIHGSSGDEFGVVGWLYARD
                     PDTGKEVWARPLVEGHIGRLNGQPSTPTGDPKAPSWPNDPNSPTGKVEAWSHGGGAPW
                     QSATFDPDTNTIIIGAGNPAPWNTHKRSPGDSLFTSGQAYVDASTGELKGFYQHTPND
                     AWDFSGNNEIVLFDYKDPKTGKTIKAGAHADRNGFFYVTDRTQLANGAGYPNKPTALL
                     NAFPFVEGITWAKHIDLKTGRPVEPTPSNRPPLPEGGEAHGKSVFVSPPFLGGKNWNP
                     MSYNPDTGLFYVAANHWGMDYWTEQVTYKPGSAYLGQGFRIKRLFDDHVGTLRAIDPR
                     TGKIVWDHKETFPFWAGTLSTAGGLLFTGTSDGYVKAFDAKTGKELWKFQTGSGIVSS
                     PVTWEQDGEQYIGLASGYGGAVPLWGGDMAQLTKQVNQGGSFWVFKLPRRAR"
     gene            6284..6874
                     /locus_tag="PKN55_06855"
     CDS             6284..6874
                     /locus_tag="PKN55_06855"
                     /inference="COORDINATES: protein motif:HMM:NF013003.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HNX70996.1"
                     /translation="MMLTLPRLALALGLAVALAHADEPPASSLAAETVVNGCRIAPKT
                     QCPGADLRHADLKAANLAGANLTGADLRRADLRMANLTGAILDGADLSGARINRGYLA
                     GASLKKARLVGADLELSRLMRADFSDADLTAASLEAARAAYAVFHRSRLIATNLQETK
                     FNGANFWEATMEGSVIRYAIFPEAFFEGCQGCPHDW"
     gene            7017..8792
                     /locus_tag="PKN55_06860"
     CDS             7017..8792
                     /locus_tag="PKN55_06860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014088302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent methanol/ethanol family
                     dehydrogenase"
                     /protein_id="HNX70997.1"
                     /translation="MNTFFARNGWRLATCTLMVSAALSAPLAGAAVNDQDILNDAKTP
                     GDVVSYGMGPQGQRFSPLTKLNTSTVKKLVPAWSFSFGGEKQRGQEAQPIIHDGVMYV
                     TASYSRAYAVDARTGQKLWEYNARLPDGIMPCCDVINRGMAIYGDLVYFGTLDARLVA
                     LDRKTGKVVWTKTLGDYKAGYSYTAAPMIVKGKIITGVSGGEFGIVGKVEARDAKTGE
                     LVWSRPVIEGHMGTLNGKESTMTGTLNATWEGDQWKQGGGATWLGGTYDPETDTIFMG
                     TGNPAPWNSWTRKGDNLYTASTLAINPDNGEIKWHLQTTPHDGWDFDGVNEFIPFDLK
                     KDGKVIKAGAKADRNGFFFVADRTNGDLLGAYPFVSKITWAKGFDLKTKKPIVNPEGR
                     PANPFDPAQQAEGGKGKSVFAAPSFLGGKNWMPMAYSPKTELFYVPSNEWGMDIWNEP
                     ITYKKGAAYLGAGFTIKPLYDDHIGVLRAMDPKTGKIKWEYKNKAPLWGGVLSTAGDL
                     VFTGTPEGFLKAFDAKTGKELWKFQTGSGVVGSPVTWEMDGEQYVSVVSGWGGAVPLW
                     GGEVAKVISHLTQGASVWTFKLPKN"
     gene            8950..9270
                     /locus_tag="PKN55_06865"
     CDS             8950..9270
                     /locus_tag="PKN55_06865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014088764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HNX70998.1"
                     /translation="MKLRWMLSAATLMLVGQAHANDPKAVEAMMQKNGCFACHSVEQK
                     IIGPAYRDVAKRYAGQADAPAKLVAKVKNGGGGVWGQMGMPPHPNLKDDDIRAMIDWV
                     LDHK"
BASE COUNT         1786 a         3037 c         2919 g         1531 t
ORIGIN      
        1 cgctaactca accccaccag cggcgcgtgc aaactcacgc acagcccgcc gccggggttc
       61 agcgccaatg tcacgccgcc gccgacactc tccagtctct cgcgcatccc cagcagacca
      121 caaccatact ttggcacgac acgcgccacg ccgttgtcgc tgaccgacac acacaagcag
      181 ccgtccgtca cctcggcgcg caattgcacg gccgtggcct ggctgtggcg ggcgatattg
      241 gtcaggcact cctgcacggc gcggtatagc tgcaaggcgt ggcgggcggg cagcggcggc
      301 atggcttgcc agtgggcgtc cagctgcact tccgggcggc gcgcctgcca ttgccgggtg
      361 agctcttcca gcgcctcggc cagcgacagg ccgtccaggc catgcgggcg cagctcgctg
      421 agcagggcgc ggatatggcg atgaatgcgc tgggtttctt gctggatgtc gcgcacgcac
      481 tggcgctgcg ggtgctccgg ctcgctgggg ccgtggcgca gcaaataggc ggcgcacacg
      541 ccaatcgcct ccagcgactg gcccagctcg tcgtgcaact cacgcgccag ctcggcttgt
      601 tcttgctcgc gcaccgccag cagccggcgg ctgagctggc gctggcgctg gcgggcggcg
      661 tgcagcttgg ccgacagggt gttcagcgtt tgcgcgatgc ggtcgaactc gcgcagggca
      721 aagcgcggca gcgtcagcgg ctggctgtcg tcttccagcg cgcggatggc ggcttcgatg
      781 cgccgggccg gcgccagcgc gcggccaatg gtcagccagc aggccaccag cgagatggcg
      841 ctgatcacca cggtgaacag gatcacgccg ctggcgtcgc ggattttttc ttcgatttcg
      901 ctgtagggct ccagcgccac atgcaccaca tggccatcca gattcagcgc aatcggctgg
      961 cggtcaaact gcacttgcgc ggccagcggc gccagccagc cgggggcttc atcccccgcc
     1021 ggcgcatccg gcccgccgcc cagacgcagg gtgacatggc gaaagccgcc agccgccacc
     1081 agctcgcgca cgcgcgccgg gttggcgtcg gcgcggccca gggcgtcgat cagttgcaat
     1141 agccgggccg aggcgcgcag ctcttcttcg gcgtcggcgc gcgcggcgtt cagccagtac
     1201 aggctgacac aggcgatcag caagagggag aacaagccca tccaggcggt caagcgcttg
     1261 cgtaaatcca tattgacccc ctactttggt tgaatcgaca tttaaatcaa tttgtgaatt
     1321 cggcagaata aacacacggg ttttctgcat ctcactctag catattccgc cgtaaatcca
     1381 tattcggcca gttcgggaaa ttttcccgac tctggcgggt gattttccct gtttaggctg
     1441 ggaacaaatc ctgatactgc cggtcaggcg gcggatgaat gggcctgatg cccggagtga
     1501 cgcacaacag cacatatccg ccaaaaacaa caatgacatg attaatatac ggatatggcc
     1561 atgctgcaat tttatcaaat cccctgtcgc ggcccggcgc gcgccccttg gttattgtgc
     1621 attgccggcc tgctgggccc ggcagtggcg caggcggaag acgccgcgct ggccgaggtc
     1681 accgtggtgg gcgccacgcc gctgccgggc ctgggggtga gccgtgcgca ggtggcggct
     1741 ccggtggtca gccgcaacgc cgccgcgctg caacacagcc aacgcgccac cctgggcgag
     1801 tttctcgagc gcgagctggg cagcgtgcat atcaacgaca tgcagggcaa cgccttgcag
     1861 gccgatgtca attaccgcgg ctacaccgcc tcgccgctgc tgggcacgcc gcagggcttg
     1921 tcggtgtatc tggacggcat gcgcatgaac cagccgtttg gcgacgtggt cagctgggat
     1981 gcgctgccgc gctcggcgct ggccacgctg acgctgatgc ccggctccaa tccgctgttt
     2041 ggcctgaaca ccctgggcgg cgcgctggcg ctgaccacca aggacggcct gagccatccc
     2101 ggcacggcga ttgaaacggg ttatggttcg gatcagcgcc gtcggctggg ttttgaacat
     2161 ggcggttcgg cggccaatgg cctgcactgg tttgtcaccg gcaactggat gaaagaaaac
     2221 ggctggcgcg agcattcgcc ctcagaaacc cgccagctgt tcgccaagct gggctggagc
     2281 ggcgagcgca ccgacctggc cgccgcgctg ggctgggccg acaacacgct gaccggcaat
     2341 ggcctgcaag acgcccgcct gctggcgcgc gactaccgca gcgtgtacac ccagccagac
     2401 gaaaccgaca accgcagcgc ctggctgaac ctcaccggca aacaccagct gaacgaccgc
     2461 gtgcagctct ctggcaatgc ctacgcgcgc cgactgagca gcagcacgct gaatggcgac
     2521 atcaacgaag attcgctgga tcagcaggtg tatttattgc gcaccagcac cgccgaccgc
     2581 aatgcgctga gcaaggccgg ctacagcaat ttgcccagcc agaacgaaac cgccgccaat
     2641 acgccgtttc cgtactggcg ctgcctggcg caaaccgcgc tggccgacga gccggcagaa
     2701 aaatgcaacg gcctgctcaa ccgcacccgc acccggcaga cgcaatacgg ctttagcggc
     2761 cagttcaccc tgcaggacac gggtaaccag ttcaccgccg ggctgggcta cgacgccagc
     2821 cgggtgcgct ttagccagac cagccagttt ggctatctga atcccgaccg tagcgtgacg
     2881 ccggtgaatt tctttgccga cggcacagaa atcgacgaca gcggcgtgcc ggtggacagc
     2941 cgggtgaacc tgagcgggcg cacccgcacc tggagcctgt tcgccaccga cacgctgacc
     3001 ctggccgagc actggcacct caccctgtcg gcgcgctgga accaaacccg cctgagcgtg
     3061 cgcgaccaca tcaccccggg cggcggcgcc ggctcgatgg acggcgacca ccgcttcagc
     3121 cgggtcaacc cggccgccgg gctgagctat gcgcccagcg ccaacctgac cgcctggctg
     3181 ggctacaacc agggcagccg cgcccccagt gtggtggagc tgggctgcgc cgaccccagc
     3241 aacccttgcc gcctgcccaa cgccatggcc agcgacccgg ccttgcgtca ggtggtcacc
     3301 cacacctggg aagccggcgt gcgcggcaaa ttcacgccga atctgcgctg gcagctgggc
     3361 ggctttcgcg ccgacagccg cgacgacatc ctgtttgtcg cgtcgcgcca gagcggttct
     3421 ggctatttca agaacgtgga ccgcacccgc cgccagggcg tcgagctggg cttgagcggc
     3481 gagcatggcg cgtttaattg ggggctggac tacacctatc tggacgccac ttaccaaagc
     3541 gcggaaaccc tcaccgccgc cgccaacagc agccaggacg ccagcggcaa tatccgtgtg
     3601 cagccgggcg accgcctgcc gttgaccccg cgccatctgg tcaagctgcg cgccgactat
     3661 cgcttttcgc cgcaatggca ggccggcgtg gccatgcgcg ccgtggcggg cagcctggcg
     3721 cgcggcaatg aaaacggcca gcaccagtcc gacggcgtat acaacctggg cccaggccgc
     3781 agcgccggct acgcggtgtg ggatttgact ggcgaatacc ggccgacgac ccaactgacc
     3841 gtgtttggcc aggtgaacaa tgtctttgac cgccgctacg ccaccgccgc ccaactgggc
     3901 gccaccggct tgaccagcgc aggcaatttt gtggcgcggc cgtttgcggc ggtgggcggc
     3961 aaatacccgc tgatgcacgc caccttctac gcccccggcg cgccgcgcag cttctggctg
     4021 ggtgtgcgct atgagtttga caaggtggcg caaaaagact gaacacgcat gaaccggctt
     4081 ctccttacag ccgccttagg gcggcttttt tatggcggat ggtgcgggat atttaaatac
     4141 cgggctcgcc tattcgcctt tagtcgtatt tttcagtgaa tagcagtttt attttgctat
     4201 cgataaatct ggttttaaat atcagcagcg catccgggaa aaaatcctgc tttgtccggg
     4261 cgtgacgccc agggttaaat catgcactcc gcttacactt gtttgtgggc aaagctgtgt
     4321 gactgcacgc atggcaaacc agacaagaca aggacggaga tatgcacacc accatcaaac
     4381 acaccctgcg gcgcgcgccg ctggctgcgg tgatttcgct ggcgctggcc ggcgccgcgc
     4441 tggccggcac ggtcagttgg gaagacatcg ccaacgacga caaaaccccg aaagacgtgc
     4501 tgatgtacgg catgggcctg aagggccagc gctttagcgc gctcaagcag gtgtccgcca
     4561 agaatgtggc cgacctggtc ccggcgtgga gcttttcgtt tggcggcgaa aaacagcgcg
     4621 gccaggaaac ccaggcgatt gtgcatgatg gggtgatcta catcaccgcc tcgtactcgc
     4681 gcatgttcgc catcgacgcc aagaccggca agcagctgtg ggcctacgag caccgcctgg
     4741 ccgacgacat ccgcccatgt tgcgacgtga tcaaccgtgg cgcggcgatt tatggcgaca
     4801 aggtgtactt tggcacgctg gacgccagca tcgtggcgct gaacaaagac accggcaaag
     4861 tggtgtggaa agaaaaattc ggcgaccaca aaatcggcta caccatgacc ggcgcgccga
     4921 ccgtggtcaa agatcaaaaa accggccgca tgctgctgat ccacggctcg tcgggtgacg
     4981 aattcggcgt ggtcggctgg ctgtacgccc gcgacccgga caccggcaaa gaagtgtggg
     5041 cgcgcccgct ggtggaaggc catatcggcc gcctgaacgg ccagccgtcc accccgaccg
     5101 gcgacccgaa agccccgtcg tggccgaacg acccgaattc gcccaccggc aaggtggaag
     5161 cctggagcca tggcggcggc gcgccgtggc agagcgccac ctttgacccg gacaccaaca
     5221 ccatcatcat cggcgccggc aacccggccc cgtggaacac ccacaagcgc tcgccgggtg
     5281 attccttgtt cacctccggc caggcctatg tggacgccag cactggcgag ctgaaggggt
     5341 tttaccagca cacgccgaac gacgcctggg acttctccgg caataacgaa atcgtgctgt
     5401 tcgactacaa agacccgaaa accggcaaaa ccatcaaggc cggcgcccac gccgaccgca
     5461 atggcttttt ctatgtgacc gaccgcaccc agctggccaa tggcgccggc tacccgaaca
     5521 aacccaccgc gctgctgaac gccttcccgt ttgtggaggg catcacctgg gccaagcaca
     5581 tcgacctgaa aaccggccgc ccggtggagc cgacgccgag caaccgtccg ccgctgccgg
     5641 aaggcggcga agcgcatggc aagagcgtgt ttgtctcgcc gccgttcctg ggcggcaaaa
     5701 actggaaccc gatgtcctac aacccggaca ctggtctgtt ctatgtggcc gccaaccact
     5761 ggggcatgga ctactggacc gagcaagtca cctacaagcc gggttcggcc tatctgggcc
     5821 agggcttccg catcaagcgc ctgtttgacg accacgtcgg caccttgcgc gcgattgacc
     5881 cgcgcaccgg caagattgtc tgggatcaca aagaaacctt cccgttctgg gccggcacct
     5941 tgtccaccgc tggcggcctg ctgttcaccg gcacctcgga tggctacgtg aaggcgtttg
     6001 acgccaaaac cggcaaggag ctgtggaaat tccagaccgg ctcggggatt gtctcctcgc
     6061 cggtcacctg ggagcaggac ggcgagcagt acatcggcct ggcctctggc tatggcggcg
     6121 cggtgccgct gtggggcggc gacatggccc agctgaccaa gcaggtgaat cagggcggca
     6181 gtttctgggt gttcaagctg ccgcgccgcg cccgctaagc ccattctcat ccaccgtttg
     6241 ccgccctggc gttcgccagg gcggatgatg acaaggattc ttgatgatgt tgactctgcc
     6301 gcgcctggcg ctggcgctgg gtctggccgt tgcgctggcc cacgccgatg aacccccggc
     6361 ctccagcctg gcggcggaaa ccgtggtcaa cggctgccgc atcgcgccaa aaacgcaatg
     6421 cccaggggcc gatttgcgcc atgccgacct caaggcggcc aatctggccg gcgccaatct
     6481 cactggcgcg gatttgcgcc gcgccgattt gcgcatggcc aacctcaccg gcgccattct
     6541 ggacggcgct gatttaagcg gcgcgcggat caatcgcggt tatctggccg gcgcctcgct
     6601 gaaaaaagcc cggctggtgg gcgcggatct ggagttgtcg cggctgatgc gcgccgactt
     6661 cagcgatgcc gacctcaccg ccgccagcct ggaagccgcg cgcgccgcgt atgcggtgtt
     6721 tcaccgcagc cgcctgatcg ccaccaattt gcaggaaacc aagttcaacg gcgccaactt
     6781 ctgggaggcg accatggaag ggtcggtgat tcgctacgcg attttccctg aggcgttctt
     6841 cgaaggctgc cagggttgtc cgcatgactg gtgatgcatc atgcgccaaa cccttgtttt
     6901 tgcaccctac ccgactttgg tcgaataggc gcagccgggt gtgctctcca caatcagagg
     6961 gccgggattt tttcccgcgc caatgaagcc caattgacaa gtggagaacc ggagagatga
     7021 acacattttt tgcgcgcaat ggctggcgac tggccacctg caccctgatg gtttccgccg
     7081 ccctgagcgc cccgctggcc ggcgctgcgg tgaatgacca ggatattctc aacgacgcca
     7141 aaacccctgg cgacgtggtg tcgtatggca tgggcccgca aggccagcgt ttcagcccgc
     7201 tgaccaagct gaacaccagc acggtgaaaa aactggtgcc ggcctggagc ttctcgtttg
     7261 gcggcgaaaa gcagcgcggt caggaagccc agccgatcat ccatgacggc gtgatgtatg
     7321 tcaccgcctc gtactcgcgc gcctacgccg tggacgcgcg caccggccaa aagttgtggg
     7381 aatacaacgc ccgtctgccg gacggcatca tgccgtgctg cgacgtgatc aaccgtggca
     7441 tggccatcta cggcgacctg gtgtacttcg gcaccctgga cgcccgtctg gtggcgctgg
     7501 accgcaagac cggcaaggtg gtgtggacca agaccctggg cgactacaag gccggttact
     7561 cgtacaccgc cgcgccgatg atcgtgaagg gcaagatcat caccggcgtg tccggcggtg
     7621 agttcggcat tgtcggcaag gtggaagccc gcgacgccaa gaccggcgag ctggtgtggt
     7681 cgcgtccggt gatcgaaggc catatgggca cgctcaatgg caaggaaagc accatgaccg
     7741 gcacgctgaa cgccacctgg gaaggcgacc agtggaagca gggcggcggc gccacctggc
     7801 tgggcggcac ctacgacccg gaaaccgaca ccatcttcat gggcaccggc aacccggcgc
     7861 cgtggaactc gtggacccgc aagggcgaca acctgtacac cgcctccacc ctggccatca
     7921 acccggacaa cggcgaaatc aagtggcacc tgcaaaccac gccgcatgac ggctgggact
     7981 ttgacggcgt gaacgaattc atcccgttcg acctgaaaaa agacggcaag gtgatcaagg
     8041 ctggcgccaa ggctgaccgc aatggcttct tctttgtggc cgaccgcacc aacggcgacc
     8101 tgctgggcgc ctacccgttt gtgtccaaga tcacctgggc caagggtttc gacctgaaga
     8161 ccaagaagcc gatcgtcaac ccggaaggcc gcccggccaa cccgtttgac ccggcccagc
     8221 aggctgaagg cggcaagggc aagtcggtgt ttgccgcacc gtccttcctg ggcggcaaga
     8281 actggatgcc gatggcctac agcccgaaga ccgaattgtt ctacgtgccc agcaacgaat
     8341 ggggcatgga catctggaac gagccgatca cctacaagaa gggcgccgct tacctgggcg
     8401 cgggcttcac catcaagccg ctgtatgacg accacattgg cgtgctgcgc gccatggacc
     8461 cgaaaaccgg caagatcaag tgggaataca agaacaaggc cccgctgtgg ggcggcgtgc
     8521 tgagcaccgc cggcgacctg gtgttcaccg gcaccccgga aggcttcctg aaggcgtttg
     8581 acgccaaaac cggcaaagag ctgtggaaat tccagaccgg ctctggcgtg gtgggcagcc
     8641 cggtgacctg ggaaatggac ggcgagcaat acgtgtcggt ggtgtctggc tggggcggcg
     8701 cagtgccgct gtggggtggc gaagtggcca aggtcatttc gcacctgacc cagggcgcca
     8761 gcgtgtggac cttcaagctg ccgaaaaact gagcatgaag taagccctgc ctggcaggcg
     8821 gcctcccccc ggacgtccgt ctgtcttgcg cccacctgat ttgggtgggc gcagatggcc
     8881 ctgagcccag ccgctgcccg gcggcggcta ggctcagatt catcccccaa acccgagagg
     8941 aagcacacca tgaaactgcg ctggatgctg tctgccgcca ccctgatgct ggttggtcaa
     9001 gcccatgcca acgacccgaa agctgttgaa gccatgatgc agaaaaacgg ctgttttgcc
     9061 tgccacagcg tcgaacaaaa aatcatcggc ccggcctacc gtgacgtggc caagcgctac
     9121 gccggccagg ccgacgcgcc ggccaagctg gtggccaagg taaaaaatgg cggcggcggc
     9181 gtttggggtc agatgggcat gcccccacat ccgaacctga aagacgatga cattcgcgct
     9241 atgattgact gggtgcttga tcacaagtaa gaa
//
LOCUS       DAOPYX010000104         9247 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_8821_length_9247_cov_11.104439, whole genome shotgun sequence.
ACCESSION   DAOPYX010000104 DAOPYX010000000
VERSION     DAOPYX010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 9247)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9247)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9247
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8821_length_9247_cov_11.104439"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            286..1428
                     /locus_tag="PKN55_06870"
     CDS             286..1428
                     /locus_tag="PKN55_06870"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNX70999.1"
                     /translation="MILSSEHQLLRDTLADFAARELAPNAARWAEAHEFPADALRKLG
                     ELGLYGITVPTEWGGAGLDTLAAAVALEEIAVGDGATSTIISVTNSVGCGPIERFGDA
                     WQKETFLRPMASGQWLGAFCLTEAQAGSDASALRTRAVRDGDDYVLNGAKQFITSGKH
                     ADIAIVIAVTDPKAGKKGLSAFIVPTATPGYQVARVEDKLGQAASDTCQIVFDQCRIP
                     ARQRLGAEGDGYRIALANLESGRIGIAAQSLGMARAALDGALRYARERESFGQPIIQH
                     QAVGFRLADLATQLEAARQLVWHAASLKDAGQPCLKEASMAKLFASEMAERVCSGALQ
                     TLGGYGYLRDFPIERIYRDVRVTQIYEGTSDIQRLVISRALVDGLL"
     gene            complement(1699..2262)
                     /locus_tag="PKN55_06875"
     CDS             complement(1699..2262)
                     /locus_tag="PKN55_06875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX71000.1"
                     /translation="MTVLSRLCRLSVAAAAVWLAACAELPAQPTSAPAATQGSATPAA
                     TPRPLNREAEVLVTLAALHEDSLNQSMTLLPLVRERAQRKPLERSIQLHANELRTLRG
                     WISRLPDRPQADYLPRLPEPGALTRAQAERQYVQAMLAHHDAVEQLARRAEGVRLRPA
                     LKTLLETVRARSQAERLALQARLDALP"
     gene            2367..>3457
                     /locus_tag="PKN55_06880"
     CDS             2367..>3457
                     /locus_tag="PKN55_06880"
                     /inference="COORDINATES: protein motif:HMM:TIGR04350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PatB family C-S lyase"
                     /protein_id="HNX71001.1"
                     /translation="MQELFQQAPDRRHSDSWKWQKYAGRDILPMWVADMDFAAPPAVQ
                     AALAERVAHGVYGYPDVLPGVAGAVSDWLAREYGWHIEADWLVWLPGLVCGLNVACRA
                     VGEPGDAVLTATPVYPPFMSAPQLSGRELQTALLQEGADGWRWDWAAMEAALTPRTRL
                     LMLCHPHNPVGRVWSAEELAEVADFARRHELTVCSDEIHCQLILDAERHHQPFASLSD
                     DAAQRSITLMAPSKTYNVPGLGCAFAIIPNPALRARFSRAMRGIVPGVNVFGLVAAEA
                     AYRDSPDWLQGLLTVLRANRERVESVLGALPGVRVTHSQGTYLAWIDVRAKGWADPVA
                     QFEAGGVGLSDGRDFGLSGFVRLNFGCPPA"
     assembly_gap    3458..3557
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3610..4695
                     /locus_tag="PKN55_06885"
     CDS             3610..4695
                     /locus_tag="PKN55_06885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012698428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HNX71002.1"
                     /translation="MERRAFLKQAGVAGLAASAIAAPAIAAEPQIRWRLASSFPKSLD
                     TIYGGAEVLADRVRELTDGKFDIRVFAGGEIVPGLQVLDAVSNGTVEIGHTCSYYYVG
                     KNKAFGFDTSMPFGLNSRQQNAWMQFGGGDKLMAELFDQYKVIALAGGNTGTQMGGWF
                     RNPIKGLVDLKGLKMRIPGIGGELMAKLGAVPQTLAGGDIYPALEKGTIDATEWVGPY
                     DDEKLGFFKIAKNYYYPGFWEPGPQITFMINKEHWAKLPKAYQAALKAAAAEANVTMQ
                     AEYDHKNPLALGRLLKQGVKLQAYPKDVMVAAQKAAEELYAEESAKNPLFKKIYASWL
                     PYRNTQSRWFSVAEGTMDRFTQATPLK"
     gene            4821..5444
                     /locus_tag="PKN55_06890"
     CDS             4821..5444
                     /locus_tag="PKN55_06890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012698429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HNX71003.1"
                     /translation="MIQAALKLSRVIDAVNDRIGRWSMWLILAMTLLSAGNAVIRKAF
                     DYSSNALLEAQWYMFSAVFLLCAGHALLHNEHIRIDLLSSRYDPRTRAWMELLGTLVF
                     LFPVVGLVLWLGVPYFLNSLRAGDVSANPGGLILWPAKMLLPLGFALLWLQGLSQAIK
                     QIGFLCGASPDPAHKGEEVSAEQALIDELRRREQAEAAAMQKGAQHG"
     gene            5437..7332
                     /locus_tag="PKN55_06895"
     CDS             5437..7332
                     /locus_tag="PKN55_06895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012698430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HNX71004.1"
                     /translation="MANVSAPAAAPVSGMRFMPWVFAFLSVLILALVGSAMMGASISD
                     VLGHDSAAHHFLAENLAPVMFASMVIFLLLGYPVAFALAANGIVFGLLGIDLGLLQPS
                     LFNAMPDRIWGVMNNDTLLAIPFFTFMGLVLERSGMAEDLLDTVGQIFGPIRGGIAYA
                     VILVGAMLAATTGVVAASVISMGLISMPIMLRYGYDRRLASGVIAASGTLAQIIPPSL
                     VLIVMADQLGRSVGDLYKGAFIPGFILTALYAVYVFACTMFRPESAPALPPEARSLRE
                     SNGASGNRSLGVLTLIAVTAGVLFAKQYPENHPLDETIVVSIMVGIGVAFVLALINRQ
                     FKLGLLSQIAEKTTFVLIPPLGLIFLVLGTIFVGIATPTEGGAMGATGAIIMALSRKR
                     LDLALLKQAMYSTAKLTTFVLFILVGARVFSLTFYGVDGHVWVEHLLHDLPGGQIGFL
                     IVVNLLVFGLAFFLDFFELAFIVVPLLAPVAEKLGIDLVWFGVLLAVNMQTSFMHPPF
                     GFALFYLRSVAATKDYTDKVTGKLIERVKSSQIYWGAVPFVLIQLAMVAMLIAFPNLV
                     TMGLDKAVDTSGAELTQELQITPDANDDALPPPVMEGEAPAAGEEPTPAFNASDFAGD
                     GKDAGKP"
     gene            complement(7506..8150)
                     /locus_tag="PKN55_06900"
     CDS             complement(7506..8150)
                     /locus_tag="PKN55_06900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009118066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HNX71005.1"
                     /translation="MDILQVLIDFFTGYGYFAVFFVLLICGFGVPIPEDITLVAGGVI
                     SGLGYTHVHTMFAVGMAGVLVGDATMFTIGRVYGKSVLKLPFIARALTPERFVAVQEK
                     FEKYGVWVLFVARFLPGLRSPIFLTAGLTRKVPFWRFLLMDGLAALISVPVWVYLGFF
                     GASNRDWLMTWVHRGQAGILVLIGLGAVLTASYWWIQRRRKARACELRRVAGRE"
     gene            8250..>9247
                     /gene="alr"
                     /locus_tag="PKN55_06905"
     CDS             8250..>9247
                     /gene="alr"
                     /locus_tag="PKN55_06905"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HNX71006.1"
                     /translation="MTRPIQARIHMDALAHNYSVAKQLAPRSQALAVIKADGYGHGYA
                     RVVRALPHADGFAMLNLENAIALRESGEIRPLMMLEGFFDREELLACAEHDIWTSVHS
                     EHQLALLESTPLPRPVSVWLKLNTGMNRLGFRPEAALAMAQRLRALPQVRLSALMTHF
                     ATADDERGIAGQMARFQPVAAALGLPVSVANSAAILRHDSARAEFIRPGIMLYGCSPF
                     AGETGEQLGLRPTMTLEADIIGVQTLQAGDEVGYGARFTAPGPMRIGVVACGYADGYP
                     RIVPSGTPVRVDGQASGTVGRVSMDMLAVDLTHLPAAGIGSKVVLWGEGVAIETVA"
BASE COUNT         1570 a         2816 c         3004 g         1757 t
ORIGIN      
        1 cccgggctgg ctgcgatgct cggcgcgctt tacggggagg taaagcggct aggcgcggcg
       61 ggcaaagcac tgaaagtatt ggttttaaat tttaaatgga cgagatgctt cacgaatggg
      121 gccctcgttg tatgcctcct cggtgtttta cccctccatc gcgtcgtccc cgcgtaggcg
      181 gggacccagg caaggcccca cagcgtggct acggtgaaat ctcgttgacc gcagtttggt
      241 gagcagctct tattttgatc gattactact gaggatggcc aaagcatgat tttgtcttcc
      301 gaacatcaat tgctgcgcga taccttggcg gattttgccg cgcgcgagtt ggcgccgaat
      361 gcggcgcgtt gggctgaggc gcatgagttc ccggccgatg cattgcgcaa gctgggcgag
      421 ttgggcttgt atggcattac cgtacccacc gagtggggcg gggcgggttt ggatactttg
      481 gcggcggcgg tggcgctgga ggaaatcgcc gtgggtgatg gcgcgacgtc caccattatt
      541 tccgtcacca attcggtggg ctgcgggccg attgagcggt ttggcgatgc gtggcaaaaa
      601 gagacgtttt tgcggccgat ggccagtggc caatggttgg gggcgttttg cctgaccgag
      661 gcgcaggccg gttcggacgc cagcgccttg cgcacccgcg cggtgcgcga tggcgatgac
      721 tatgtgctca acggcgccaa gcagttcatc acctcgggca agcacgccga catcgccatc
      781 gtgattgccg tcaccgaccc gaaagccggc aaaaaaggcc tgtcggcgtt tattgtgccc
      841 accgccacgc cgggttatca ggtggcgcgg gtggaggaca agctgggtca ggcggccagt
      901 gacacctgcc agattgtgtt tgaccagtgc cgcattcccg cccgccagcg gctgggcgcg
      961 gagggcgatg gctaccgcat tgccctggcc aatctggaat ccgggcgcat cggcattgcc
     1021 gcccagtcgc tgggcatggc gcgcgcggcg ctggatggcg cgctgcgcta cgcccgcgag
     1081 cgggaaagct ttggccagcc gattatccag caccaggcgg tgggctttcg cctggccgac
     1141 ctggccactc agctggaagc ggcgcggcag ttggtgtggc acgccgccag cctgaaagac
     1201 gccggccagc cctgcctgaa agaagcctcg atggccaagc tgttcgcctc ggaaatggcc
     1261 gagcgggtgt gttccggcgc cttgcagacg ctgggcggct acggctattt gcgcgatttt
     1321 ccgattgagc ggatttaccg cgacgtgcgg gtgacgcaga tttacgaagg caccagcgat
     1381 attcagcgcc tggtgatcag ccgggctttg gtggacgggc tgctgtaagc gtcgctcagc
     1441 aaacccgcct ggcgtggttg cccgaccttg ccgcactccc cgcgcgcctg gccaggcgcg
     1501 ctgcgctgga tttggtgagc aacgctaaac caaaccaggc cgccgctgtt tcctctcatg
     1561 tcatccggcg ctgccccgcc ggatagtgtg gcgtttccgg gctactctcc tcaagtaccc
     1621 gctttggccc accctgcaag gggtgggctt tttcttgcgg ggtagatcca atagcgtctg
     1681 cgcgccagca aaagacgatc agggtagcgc atccagccgg gcctgtagcg ccaggcgttc
     1741 ggcctggctg cgcgcccgca ctgtttccag cagggttttc aacgccgggc gcagccgtac
     1801 gccctcggca cggcgggcca attgctcgac cgcatcgtga tgcgccagca tggcttgcac
     1861 atactggcgt tcggcctggg cgcgggtcag ggcgccgggt tcgggcaggc gcggcaaata
     1921 atcggcctgt ggccggtcgg gcaggcggct gatccagcca cgcaaggtgc gcagctcgtt
     1981 ggcgtgcagc tggatgctgc gctccagcgg cttgcgctgg gcgcgctcgc gcaccagggg
     2041 cagcagcgtc atgctttgat taaggctatc ttcgtgcagg gcggccaggg tgaccagcac
     2101 ttcagcttcg cggttgagcg gtcgcggggt ggcggcgggg gtggcgctgc cttgagtggc
     2161 ggccggggcc gaggtgggct gtgccggcag ctcggcgcac gcggccagcc acacggcggc
     2221 ggcggccacc gacaggcggc acagtcggga taatacggtc atacaagctc ctgtcttgcc
     2281 ggccacacgg ccggcgggca tgatggttcg gttatgaacc ggccgctggg caattgttcc
     2341 cacggcccag ccaggtggac atcatcatgc aggagctttt ccaacaagcg cccgaccggc
     2401 gccacagcga cagctggaaa tggcagaaat acgccggccg cgacattttg ccgatgtggg
     2461 tggccgatat ggactttgcc gcgccgccgg cagtgcaagc cgcgctggcc gaacgggtgg
     2521 cgcacggcgt gtatggctat ccggacgtgc tgcccggcgt ggccggcgcg gtaagtgact
     2581 ggctggcgcg tgaatacggc tggcacattg aggccgattg gctggtgtgg ctgcctggcc
     2641 tggtgtgcgg gctgaatgtg gcctgccgcg cggtgggcga gcctggcgac gcggtgctga
     2701 ccgccacgcc ggtgtatccg ccgtttatgt cggcgccaca gttatcgggc cgtgagctgc
     2761 aaaccgcgct gctgcaagaa ggcgccgatg gctggcgctg ggattgggcg gccatggaag
     2821 ccgcgctgac cccgcgcacc cgcctgttga tgctgtgcca tccgcacaac ccggtgggcc
     2881 gggtgtggtc ggccgaggag ctggccgaag tggccgattt tgcccgccgc catgaactga
     2941 cggtgtgctc ggacgagatt cactgccagt tgattctgga cgccgagcgc caccaccagc
     3001 cgtttgccag cctgtcggat gacgccgccc agcgcagcat cacgctgatg gcgccctcaa
     3061 aaacctataa cgtgcccggc ctgggctgcg cttttgccat tatccccaac ccggctttgc
     3121 gcgcgcgctt tagccgcgcc atgcgcggca ttgtgccggg ggtgaatgta tttggcctgg
     3181 tggcggccga agccgcctat cgcgacagcc cggattggct gcaaggcttg ttgacggtgc
     3241 tgcgcgccaa ccgggagcgg gtggaaagcg tgctgggcgc gctgcccggc gtgcgcgtca
     3301 cccacagcca gggcacctac ctggcctgga ttgatgtgcg cgccaagggc tgggccgacc
     3361 cggtggcgca gtttgaggcc ggcggcgtgg gcttgtcgga cgggcgggat tttggtctgt
     3421 ccggttttgt ccgcctgaac tttggctgcc cgccggcnnn nnnnnnnnnn nnnnnnnnnn
     3481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3541 nnnnnnnnnn nnnnnnnaca ggcggataaa aaaatcatca catgagaagt gaacacaaag
     3601 gagaaactcg tggaacgtcg cgcctttctg aaacaagccg gggtcgccgg cctggccgca
     3661 tccgccatcg ccgcgccggc gattgccgct gaaccgcaaa tccgctggcg gctggcgtcc
     3721 agcttcccga aaagcctgga caccatctat ggcggcgctg aagtgctggc cgaccgcgtg
     3781 cgcgagctga ccgacggtaa attcgacatc cgcgtgttcg ctggcggcga aatcgtgccg
     3841 ggcctgcaag tgctggacgc agtcagcaat ggcacggtgg aaatcggcca cacctgcagc
     3901 tattactatg tcggcaagaa caaggcgttt ggctttgaca cctcgatgcc gtttggcctg
     3961 aattcccgcc agcaaaacgc ctggatgcag tttggcggcg gcgacaagct gatggccgag
     4021 ctgtttgacc aatacaaggt gattgccctg gctggcggca ataccggcac gcagatgggt
     4081 ggctggttcc gcaatccgat caaaggcctg gtcgacctca agggcctgaa aatgcgtatc
     4141 cccggcattg gcggcgagct gatggccaag ctgggcgcgg tgccgcaaac cctggccggt
     4201 ggcgatatct acccggcgct ggaaaaaggc accatcgacg ccaccgaatg ggtggggccg
     4261 tacgatgatg aaaagctggg cttcttcaag atcgccaaaa attactacta tccgggcttc
     4321 tgggagccgg gcccgcagat caccttcatg atcaacaaag agcactgggc caagctgccc
     4381 aaggcttacc aggccgcgct gaaggccgcc gccgccgaag ccaacgtcac catgcaggcc
     4441 gaatacgacc acaaaaaccc gctggcgctg ggccgtctgc tcaaacaggg cgtcaagctg
     4501 caagcctatc cgaaggacgt gatggtggcg gcgcagaagg ccgcagaaga actgtacgcc
     4561 gaagaatcgg ccaagaaccc gctgttcaag aagatctacg cctcctggct gccctaccgc
     4621 aacacccagt cgcgctggtt cagcgtggcg gaaggcacca tggaccgctt cacccaggcc
     4681 actccgctga agtaagcgcc gacgacccga tgcaaaccaa cgcgaccgtc acccccggtc
     4741 gcgttgttgt ctgtgtcgcg ctggtccctc tggcccgaat cgggcctcag gccaccctgg
     4801 ctacccatga ggtacgttaa atgattcaag ctgccctgaa actgtcgcgc gtgatcgacg
     4861 cggtcaacga cagaatcggc cgctggagca tgtggctgat tctggccatg accctgctca
     4921 gcgccggcaa tgcggtcatc cgcaaagctt ttgactatag ctccaatgcg ctgctggaag
     4981 cgcaatggta tatgttctcc gccgtgtttc tgctgtgcgc cggccatgcc ctgctgcaca
     5041 acgagcacat ccgcattgat ttgctgtcgt cccgctacga cccgcgcacc cgcgcctgga
     5101 tggaattgct gggcaccctg gtgtttttgt tcccggtggt gggcctggtg ctgtggctgg
     5161 gcgtgccgta ctttttgaat tcactgcgcg ccggcgatgt gtcggccaac cccggcgggc
     5221 tgattttgtg gccggccaaa atgctgctgc cgctgggctt tgccctgctg tggctgcaag
     5281 gcttgtcgca ggcgatcaag cagatcggct ttttgtgcgg cgccagcccg gatccggccc
     5341 acaagggcga agaggtcagc gccgaacagg ccttgattga cgagctgcgc cgacgcgaac
     5401 aagccgaagc cgccgccatg cagaaaggag cgcaacatgg ctaatgtctc tgcccccgcc
     5461 gccgcgccag tcagcggcat gcgctttatg ccctgggtgt ttgccttttt gtcggtattg
     5521 atccttgccc tggtcggctc ggcgatgatg ggcgcctcca tcagcgacgt gctgggccac
     5581 gacagcgccg cgcatcattt tttggcggaa aacctggcgc cggtgatgtt cgcctcgatg
     5641 gtgatttttt tgctgctggg ctatccggtg gcgtttgccc tggccgccaa cggcattgtg
     5701 tttggcctgc tgggcatcga cctgggcctg ttgcagccct cgctgttcaa cgccatgccc
     5761 gaccgcatct ggggggtgat gaataacgac accctgctgg cgattccgtt cttcaccttc
     5821 atgggcctgg tgctggagcg cagcggcatg gccgaagact tgctggacac cgtcggccag
     5881 attttcgggc cgattcgcgg cggcattgcc tatgcggtga ttctggtggg cgccatgctg
     5941 gcggccacca cgggcgtggt ggcggcaagt gtgatttcga tggggctgat ttccatgccc
     6001 atcatgctgc gctatggcta cgaccgccgg ctggcctcgg gtgtaattgc cgcttcaggc
     6061 accctggcgc agatcatccc gccgtcgctg gtgctgattg tgatggccga ccagctgggc
     6121 cgttcggtgg gcgatttgta caagggtgcg tttattccgg gctttatcct gacggctttg
     6181 tacgctgtgt atgtgtttgc ctgcaccatg ttccgcccag agtctgcccc ggccttgccg
     6241 cccgaagcgc gctcgctgcg cgagtccaat ggcgcatcgg gcaaccgctc gctgggtgtg
     6301 ctcaccctga ttgccgtcac cgctggcgtg ctgttcgcca agcaataccc ggaaaaccac
     6361 ccgctggacg aaaccattgt ggtgtccatc atggttggca ttggcgtggc ctttgtgctg
     6421 gcgctaatca accgccaatt caagctgggc ctgctgtcgc agatcgccga aaaaaccacc
     6481 tttgtgctga ttccgccgct ggggctgatt tttctggtgc tgggcacgat ttttgtgggc
     6541 atcgccaccc ccaccgaagg cggcgccatg ggcgccacgg gggccatcat catggccttg
     6601 tcgcgcaagc ggctggattt ggcgctgctc aagcaggcga tgtacagcac cgccaagctg
     6661 accacctttg tgctgttcat cctggtgggc gcgcgggtgt tcagcctgac cttctacggc
     6721 gtggatggcc atgtctgggt ggagcatctg ctgcacgacc tgccgggcgg ccagattggc
     6781 ttcttgattg tggtcaacct gctggtgttc ggcctggctt tcttcctgga ttttttcgag
     6841 ctggccttta tcgtggtgcc cttgctggcc ccggtggcgg aaaaactcgg cattgacctg
     6901 gtgtggtttg gcgtcttgct ggcggtgaat atgcaaacct cgttcatgca cccgccgttt
     6961 ggctttgcgc tgttttacct gcgcagtgtg gccgccacca aggactacac cgacaaggtc
     7021 accggcaagc tgatcgagcg ggtgaaatcc agccagattt actggggggc agtgccgttt
     7081 gtgctgattc agctggcgat ggtggccatg ctgattgcct tccccaatct ggtcaccatg
     7141 ggcctggaca aggcggtgga caccagcggc gccgagctga cgcaagagct gcaaatcacc
     7201 ccggacgcca atgacgacgc cctgccgccg ccggtgatgg aaggcgaagc gccagcggcc
     7261 ggcgaagagc ccaccccggc gttcaacgcc agtgattttg ccggtgatgg caaggatgcc
     7321 ggcaagccgt aatctggcta agcgactggc taatggtgtg aaaaagccct gcattgttac
     7381 ccgatgcagg gctttgctgt gtaaaacggg agcgcaaacc tcagtggtag aagttgtcgc
     7441 cagcggacac tgtcggtgca aggcgaccgt cagtgccaga tgcgggttgg cagccaggcg
     7501 gcggtttatt cccgccccgc cacccggcgc aattcacagg cgcgggcttt gcgccggcgc
     7561 tgaatccacc aatagctggc cgtcagcacg gcgcccaggc caatcagcac caaaatgccg
     7621 gcctggccac ggtgcaccca ggtcatcagc caatcgcggt tggaggcgcc aaaaaagccc
     7681 agatacaccc ataccggcac gctgatcagc gcggccaggc cgtccatcag caaaaagcgc
     7741 cagaatggca ccttgcgggt gagcccggcg gtcagaaaaa tcggcgagcg caagcccggc
     7801 agaaaacggg cgacaaacag cacccatacc ccgtactttt caaatttttc ctgcacggcg
     7861 acaaagcgtt ccggcgtcag cgctctggcg ataaaaggca gtttcagcac ggacttgccg
     7921 tacacccggc caatggtgaa catggtggcg tcgcccacca gcacgccggc catgccgacg
     7981 gcgaacatcg tatgcacatg agtgtagccc aggccagaga tcaccccgcc ggccaccagg
     8041 gtgatgtcct ccgggatcgg cacgccaaaa ccacagatca gcagcacgaa aaatacggcg
     8101 aaataaccat atccagtgaa aaagtcgata agtacttgca aaatgtccat ttgacgtcct
     8161 cgggcgcgtc aggggcgctc ccgctggcgg tgagtgtact ccaagcggcg tataatggcc
     8221 cactcctgat gtgaattccc cgcacgctca tgacccgtcc gattcaagcc cgcatccata
     8281 tggatgccct cgcccacaat tattccgtgg ccaagcaact tgccccgcgc agccaggcgc
     8341 tggcggtgat caaggccgat ggctatggcc atggctacgc ccgcgtggtg cgcgccctgc
     8401 cgcatgctga cggctttgcc atgctcaacc tggaaaacgc cattgccttg cgcgagtcgg
     8461 gagaaatccg accgctgatg atgctggaag gtttctttga tcgcgaagaa ctgctggcct
     8521 gcgccgagca cgacatctgg acctcggtgc acagcgagca tcagctggcg ctgctggaat
     8581 ccaccccgct gccgcgcccg gtcagcgtct ggctcaagct caacaccggc atgaaccggc
     8641 tgggtttccg cccggaagcc gcgctggcca tggcccagcg cctgcgcgcc ttgccccagg
     8701 tgcgcctgag cgcactgatg acccactttg ccaccgccga tgacgagcgc ggcattgccg
     8761 gccagatggc gcgttttcag ccggtggcgg ccgcgctggg cctgccggtc agcgtggcca
     8821 attcggcagc gattttgcgc cacgactcag cccgcgccga attcattcgc cccggcatca
     8881 tgctgtacgg ctgctcgccg tttgccggcg aaaccggcga acagctgggc ctgcgcccga
     8941 ccatgacgct ggaggccgac atcatcggcg tgcaaacctt gcaagccggc gacgaagtgg
     9001 gctatggcgc gcgctttacc gcgccagggc cgatgcgcat tggcgtggtg gcctgtggtt
     9061 acgccgatgg ctacccgcgc attgtgccct cgggcacgcc ggtgcgggtg gatggccagg
     9121 ccagcggcac ggtgggccgg gtgtcgatgg atatgctggc ggtggacctc acccacctgc
     9181 ctgccgccgg catcggcagc aaggtggtgt tgtggggtga gggcgtggcg attgaaacgg
     9241 tggcgga
//
LOCUS       DAOPYX010000105         9208 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_8868_length_9208_cov_5.839397, whole genome shotgun sequence.
ACCESSION   DAOPYX010000105 DAOPYX010000000
VERSION     DAOPYX010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 9208)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9208)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9208
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8868_length_9208_cov_5.839397"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            310..1080
                     /gene="rfbF"
                     /locus_tag="PKN55_06910"
     CDS             310..1080
                     /gene="rfbF"
                     /locus_tag="PKN55_06910"
                     /EC_number="2.7.7.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018150482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047343 - glucose-1-phosphate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate cytidylyltransferase"
                     /protein_id="HNX71007.1"
                     /translation="MKAVILAGGFGTRISEESHLRPKPMIEIGGKPILWHIMKIYSHY
                     GINDFVICLGYKGYMIKEYFANYFLHMSDVTFDMSSNQMHVHHKSAEPWKVTLVDTGE
                     SSMTGGRLRRVREYLDDDAFCFTYGDGVADVDIGKLVKFHRTHGKLATVTAIQPPGRY
                     GALAMDEDMVLGFQEKPKGDGGWINGGFFVLSPKVLDYIDSDDSTWEQEPLITLAEQG
                     QLLAYKHEGFWQAMDTLREKNLLEDLWQSKTAPWKVWK"
     gene            1041..2162
                     /gene="rfbG"
                     /locus_tag="PKN55_06915"
     CDS             1041..2162
                     /gene="rfbG"
                     /locus_tag="PKN55_06915"
                     /EC_number="4.2.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012698160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047733 - CDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glucose 4,6-dehydratase"
                     /protein_id="HNX71008.1"
                     /translation="MAKQNRAVESLEMTAPVLPNPAFWRGRKVFLTGHTGFKGGWLAL
                     WLQQLGADVTGYALAPDTEPALFDLADVAHGMRSVIADIRDADAVALALASAQAEIVL
                     HLAAQPLVRASYADPLTTYATNVMGTANVLEASRRVPSVKVVQVITTDKCYDNREWEY
                     PYRETDRLGGHDPYSNSKACAELVVDSYRRSFMREAGIHLASARAGNVIGGGDWAVDR
                     IVPDCIRALLAEQPIDIRSPGAIRPWQHVLEPLSGYLVLAEQQYADAAGALASGFNFG
                     PESSGAVPVGELAAGIVRHWGQGQLNISGDPHKLHEAGTLKLDISKARVRLGWRPRWD
                     FDKTLHHTVDWYRRVLCHGESARQVCLAQIADYQTLAQP"
     gene            2181..3413
                     /locus_tag="PKN55_06920"
     CDS             2181..3413
                     /locus_tag="PKN55_06920"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012698161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNX71009.1"
                     /translation="MSRHCRFCHTPLTHTFADLGLSPLSNSYVKAERLNQGEMFYPLH
                     AWVCPSCKLVQLEEFERAENIFNDEYAYFSSYSSSWLAHARQYSEAMTARFGLNAASQ
                     VVEIASNDGYLLQNFVAAGIPALGVEPTGNTAEVAIGKGIPTWVKFFGVATAHELVAA
                     GHAADLLLGNNVLAHVPDINDFVGGLKVALKPGGVITMEFPHLLNLIRHNQFDTIYHE
                     HFSYLSLLAVERIFAHHGLALFDVQELPTHGGSLRIFAQHANGPHATQPGLQAVRDKE
                     AADGLDELDIYARFAAQVDETKRALLRFLIDAKEQGLRVAGYGAPAKGNTLLNYCGVR
                     TDLIDFTVDASPHKQGTWLPGTRIPVYAPEKLREAKPDIVLILPWNLKDEIVAAHSYI
                     SEWGGRFAVPIPRVEFVA"
     gene            3410..3940
                     /gene="rfbC"
                     /locus_tag="PKN55_06925"
     CDS             3410..3940
                     /gene="rfbC"
                     /locus_tag="PKN55_06925"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: protein motif:HMM:NF013103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HNX71010.1"
                     /translation="MTLTETPIAGAWLLEIQPISDERGFFARSLCVDTLAAHGLEGRM
                     QQQSVSFNQRRGTLRGMHYQSAPHEEIKLVRVTQGRIYDVILDLRRDSPSYLRWHAVE
                     LSADNHRTLYIPKGVAHGFQTLEDHSEVFYQMAQAYVPGHSCGVRWDDPAFAIDWPLA
                     APILSERDASYPNFSS"
     gene            3955..5019
                     /locus_tag="PKN55_06930"
     CDS             3955..5019
                     /locus_tag="PKN55_06930"
                     /inference="COORDINATES: protein motif:HMM:NF016908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HNX71011.1"
                     /translation="MDNQKLTQALASIAANPAWKDAFIKHILNFEKDVATPGVDVRED
                     ISGPLVDALFDDDQVVRKQIADGTVFEFLYRSKIARDFIMSEPSVPDHAWEPHTSRLL
                     VTLAKGAKNVVVGGAYFGDQSILIAKEAAKSGGVVHAFEPNNDQRKMLMHNAELNQLS
                     NIVPRSEGLWDNSTTTLALVGYDSFAHPETVDAAHDDAFQTVTIDEYLRAAGVDRLDL
                     IMLDIEGAEYRALKGAQSFLQQPAGQAPNIVFEVHRHYVDWSNGLENTELIRFLTDLG
                     YQVFAVRDFNSNYNLAGKPIEIIPAKDVYLEGPDHGFNMVAVKDVSVFDNPGFRICHG
                     LSPKLLRHKDPALHHPTDGL"
     gene            5025..5840
                     /locus_tag="PKN55_06935"
     CDS             5025..5840
                     /locus_tag="PKN55_06935"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HNX71012.1"
                     /translation="MAKAPAYTVLGASGFVGQRLVSELTRRGADFWVPARQAPEILQQ
                     PLGVVFYCIGLTADYAQRPFDTVAAHVSYLARILEEAQFEHIVYLSSTRLYDGLPIAE
                     GRGDTPLSLSPNNPRHLYDLSKALGENLCQTTAASRSSIARLSCVYDAEPGSPGFLSE
                     LLQRLRHERAFTLDSSPGYCRDYITLDDTVASLIAMGEQRACDIFNVASGVNTFNQDI
                     ADALRPLGVDIRFSRSGEAMQLARCHIDNLRALGVAPADTLHTLQTLVRNLPA"
     gene            5837..6499
                     /locus_tag="PKN55_06940"
     CDS             5837..6499
                     /locus_tag="PKN55_06940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX71013.1"
                     /translation="MTASLTLRRLDQEHLAQFIARQLEHFVPDGLPINLPLIRQHLPQ
                     ALERLRVCINAVRWWKENEFDHLHSSQYCIFLYYLANTLWKETGDTELPTKLFILNKA
                     LNAIDLFYEIDMPARFFIGHSAGIVLAKASYADYLVLYQNSTVGKNHGVAPVIESGVI
                     MYPNTAIIGRCHVRAGTVISQGVGVVNQDTPGNCLVFRGVEQPLLFKPCERNILADIF
                     RL"
     gene            6574..8733
                     /locus_tag="PKN55_06945"
     CDS             6574..8733
                     /locus_tag="PKN55_06945"
                     /inference="COORDINATES: protein motif:HMM:NF025214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNX71014.1"
                     /translation="MADTSLDPLRRAANSDPLHAGKRYQLAISLIQHAQAISLASPQC
                     TQWLQEASEHLNRIVAQDPLHVDAWAMLGYLNIYRNRPEDAEPAVRRALALNPNHAAS
                     LVNLSNLQLGQRQFDEARATLEHAVRATPHNPWAHYNLGQACLKMGHSEAALAHTQRA
                     FALLPDNKDILDALLLQSVYTDQMDDQEKFALHRQYERLANPDGHTFSSHRNDPNPQR
                     ALRLGLVSGDFVHASLMYVTLPVLTRLAHMGFQLFFYHNGVAVDAVTEQLQAIAGAQW
                     RFTKGLDADTMCALVQDDQIDLLVDMSGHVEHNFLTAFARKPAPVQLSWAAYPATTGL
                     SSIDYVITDPTLWPPSSEQQYWTEQPCHMPDCFVVYQPYLRNPQRGQDPAYTVRPSPA
                     LRQGWVTFGSGTSLARLTPSTVTLWTRVLNAVPDSRLKLECNCSPDIVKALFIAQGVS
                     ATRIEASERTADTQYLFYHDIDIALDGAPSNGGVTSLDTLWMGVPLVTLRGNAMASRL
                     GASFLTQLGRSDWIADSADEFVTIASALAADLPALEHARQTLRPAMQASPLMNAAHFT
                     EAFSQRLRSMWQTWCASPDAATARDAQQQHEALALCEALMQQDPASALQAYQMVLKEW
                     PDNLDALYGLGLALLLNQHTEQAITILEKAREVFSAHGPAPVHAECLAALGQAWLLQG
                     HLDQARLCFQASQQICPSAAVQQWLSEIENPHTLPSV"
BASE COUNT         1859 a         2925 c         2641 g         1783 t
ORIGIN      
        1 ttaattgcta cttggaccaa gagcaccaag catttctggc ttttccacgc acggtacaag
       61 cacagctcga catgatcacc catcacacat agactgccat cgtacgatga atcgcgcaca
      121 ccgctcgttc ttcattcagt caagagtgga gcagaaccta acggtgtaca cggtaacatc
      181 tttagagttg atcgcgcagc actcggggtt catttttacc gaagaaagcc gatacagggc
      241 aagaggcaca gcgccatgcg ggtcaggtcg tcagatctgg agtcaacaca aacacggtag
      301 ttacagtcta tgaaagcagt catcctcgcc gggggctttg gcacccgcat cagtgaagag
      361 tcccacctgc gccccaagcc gatgatcgaa atcggcggca agcccattct gtggcatatc
      421 atgaagatct attcccacta tggaatcaat gacttcgtga tctgtctggg ctacaagggc
      481 tatatgatca aggagtactt cgccaactac ttcctgcaca tgtccgacgt cactttcgac
      541 atgagcagca accagatgca cgtacaccac aagagcgccg aaccgtggaa agtcaccctg
      601 gtggacaccg gcgagagctc gatgaccggc ggtcgtctgc gccgggtgcg cgagtacctg
      661 gatgacgacg ccttttgctt cacctatggc gatggcgtgg ccgatgtgga tatcggcaag
      721 ctggtgaagt tccatcgcac ccacggcaag ctggccactg tcaccgccat ccagccgcca
      781 ggccgctatg gcgcgctggc catggatgaa gacatggtgc tgggctttca ggaaaaaccc
      841 aagggagatg gcggctggat caatggcggc ttttttgtgc tgtcgcccaa ggtgcttgat
      901 tacatcgaca gcgacgacag cacttgggaa caagagccgc tgatcaccct ggccgaacag
      961 ggccaactgt tggcttacaa acacgaaggc ttctggcaag ccatggacac cctgcgcgag
     1021 aaaaacctgc tggaagacct gtggcaaagc aaaaccgcgc cgtggaaagt ctggaaatga
     1081 ccgcgccggt gttgcccaac ccagccttct ggcgcggccg caaggtgttc ctgaccggcc
     1141 acacaggctt caagggcggt tggctggcgc tgtggctgca acagctgggc gccgatgtca
     1201 ccggttatgc gctggcgcca gacaccgaac cggcgctgtt tgatttggcc gatgtcgccc
     1261 acggcatgcg ctcggtgatt gccgacatcc gcgacgccga cgccgtggcg ctggcgctgg
     1321 ccagcgccca ggcagaaatc gtgctgcatc tggccgcgca accgctggtg cgcgcctctt
     1381 acgccgaccc gctgaccact tacgccacca atgtgatggg cacggccaat gtgctggaag
     1441 cctcacgccg ggtgccgtcg gtcaaagtgg tgcaggtgat caccaccgac aagtgctacg
     1501 acaaccgcga gtgggaatac ccctaccggg aaaccgaccg actgggcggc cacgacccgt
     1561 acagcaacag caaggcctgc gccgagctgg tggtggacag ctatcgccgc tcgttcatgc
     1621 gcgaggccgg cattcatctg gcctcggcgc gcgccggcaa tgtgattggc ggcggcgact
     1681 gggcggtcga tcgcatcgtg ccggactgca tccgcgcgct gctggccgaa cagcccatcg
     1741 acatccgctc acccggcgcc atccgcccct ggcaacatgt gctggagccc ttgtctggct
     1801 atctggtgct ggccgagcag caatacgccg atgcggcggg cgcgctggcc agcggcttta
     1861 actttggccc ggaatccagc ggcgccgtgc cggtgggcga actggccgcc ggcattgtcc
     1921 gccattgggg ccagggccag ctgaacatca gcggcgaccc gcacaagctg cacgaggccg
     1981 gcacgctgaa gctggacatc agcaaggcgc gcgtgcgcct gggctggcgt ccgcgctggg
     2041 atttcgacaa aaccctgcac cacacggtag actggtatcg tcgagtgctt tgtcatggcg
     2101 aatccgcccg ccaggtgtgc ctggcgcaga ttgccgacta ccagaccctc gcccaacctt
     2161 aactcccaag cgtgaatcac atgtcccgtc attgccgctt ctgtcatacg ccgctgaccc
     2221 acacttttgc cgacctgggc ctgtcgccgc tgtccaattc ttatgtgaaa gccgagcggc
     2281 tgaaccaggg ggaaatgttc tacccgctgc acgcctgggt gtgccccagc tgcaaactgg
     2341 tgcaactgga agaattcgag cgcgcagaaa atattttcaa cgacgaatac gcctattttt
     2401 cctcgtactc cagcagctgg ctggcgcacg cccgtcagta cagcgaggcg atgaccgccc
     2461 gctttggcct gaacgctgcc agccaggtgg tggaaatcgc cagcaatgat ggctatctgc
     2521 tgcaaaactt tgtcgccgcc ggcatcccgg cgctgggcgt ggagccaacc ggcaacaccg
     2581 ccgaagtggc catcggcaag ggcatcccca cctgggtgaa gttctttggc gtcgccaccg
     2641 cccatgagct ggtcgccgcc ggccacgccg ccgatttgct gctgggcaat aatgtgctgg
     2701 cccacgtgcc ggacatcaat gattttgtcg gcggcctgaa agtggcgctc aagccgggcg
     2761 gggtgatcac catggaattc ccccacctgc ttaacctgat tcgccacaat cagttcgaca
     2821 ccatctatca cgagcacttc tcttatttgt cgctgctggc ggtggagcgt atttttgctc
     2881 accacggcct ggcgctgttc gacgtgcaag aactgcccac ccacggcggc tcgctgcgca
     2941 tctttgccca gcacgccaat ggcccgcacg ccacccagcc cggcctgcaa gcggtgcgcg
     3001 acaaagaagc cgcagacggc ctggacgagc tggacatcta cgcccgcttt gccgcccagg
     3061 tggacgaaac caagcgcgcg ctgctgcgct ttttgatcga cgccaaagag caaggcctgc
     3121 gcgtggccgg ctacggcgcg ccagccaagg gcaacaccct gctgaactac tgcggcgtgc
     3181 gcaccgacct gatcgacttc accgtggacg ccagcccgca caagcaaggc acctggctgc
     3241 ccggcacccg cattccggtg tacgccccgg aaaaactgcg cgaagccaag ccggacatcg
     3301 tgctgatcct gccgtggaac ctcaaggacg aaatcgtcgc cgcacacagc tatatcagcg
     3361 aatggggcgg gcgctttgcc gtgccgattc cgcgcgtgga gtttgtggcg tgacgctgac
     3421 cgaaacgccg attgccggcg cctggctgct ggaaattcag cccatcagcg acgagcgcgg
     3481 cttttttgcc cgcagcctgt gcgtggacac cctggccgcg cacggcctgg aaggacgcat
     3541 gcagcagcaa agcgtgtcgt tcaaccagcg gcgcggcacg ctgcgcggca tgcattacca
     3601 aagcgccccg cacgaagaaa tcaagctggt gcgcgtcacc cagggccgca tttacgatgt
     3661 gattctggac ttgcgccgcg actcgcccag ctatctgcgc tggcatgcgg tggagctgtc
     3721 ggccgacaac caccgcacgc tgtacatccc caaaggcgtg gcgcacggct ttcagaccct
     3781 ggaagaccac tcggaagtgt tttaccagat ggcgcaggcc tatgtgcctg gccacagctg
     3841 cggtgtgcgc tgggatgacc cggcgttcgc catcgactgg ccgctggcgg cgccaatcct
     3901 atcggaacga gacgccagct accctaactt ttcctcttga tgaagatcac gctcatggat
     3961 aatcagaaac tgactcaagc gctggcgagc attgccgcca acccggcctg gaaagacgct
     4021 ttcatcaagc atatcctgaa ttttgaaaaa gacgtcgcca cccctggcgt ggacgtgcgc
     4081 gaagacatca gcggcccgct ggtggatgcc ctgttcgacg atgatcaagt ggtgcgcaag
     4141 cagattgccg acggcacggt gtttgaattt ttgtaccgct cgaaaatcgc ccgtgatttc
     4201 atcatgtcgg aacccagcgt gcccgaccac gcctgggagc cgcacacctc gcgcctgctg
     4261 gtcaccctgg ccaaaggcgc caagaatgtg gtggtcggcg gcgcttactt tggcgaccag
     4321 tccatcctga tcgccaaaga agcggccaaa tccggcggtg tggtgcacgc tttcgagccg
     4381 aacaacgacc agcgcaaaat gctgatgcac aacgccgagc tgaaccagct gagcaatatt
     4441 gtgccgcgca gcgaaggcct gtgggataac agcaccacca cgctggcgct ggtgggctac
     4501 gactcgtttg cccacccgga aaccgtggac gccgcacacg acgatgcttt ccagaccgtc
     4561 accatcgacg aatacctgcg cgctgccggt gtggatcgtc tcgacctgat catgctggac
     4621 atcgaaggcg ccgaataccg cgcgctcaag ggcgcacaga gcttcctgca acagccggca
     4681 ggtcaagcgc ccaatatcgt gtttgaagtg catcgccact atgttgactg gagcaacggc
     4741 ctggaaaaca ccgagctgat ccgcttcctg actgatctgg gctatcaggt gtttgccgtg
     4801 cgcgacttta actccaatta caatctggca ggcaagccga ttgaaatcat tccggccaag
     4861 gacgtgtatc tggaaggccc ggatcatggc ttcaatatgg tggcggtcaa ggacgtcagc
     4921 gtgttcgaca acccgggctt ccgcatctgc catggcctca gccccaagct cttgcgccat
     4981 aaggacccag ctctgcatca cccgaccgac ggcctgtaag cgccatggcc aaggcccccg
     5041 cctacaccgt tttgggcgcc agcggctttg tcggccagcg tctggtcagc gagctgaccc
     5101 gacgcggcgc cgacttttgg gttccggcgc ggcaagcgcc ggaaatcctg caacagccgc
     5161 tgggcgtggt gttttactgc atcggcctga ctgctgatta tgcccagcgt ccgtttgaca
     5221 cggtagcagc gcatgtcagc tatctggcgc gcattctgga agaagcgcag ttcgagcata
     5281 ttgtttatct gtcttccacc cgcctgtatg acggcctgcc gattgccgag ggacgtggcg
     5341 acaccccgtt gagcctgagc ccgaacaacc cacgtcattt atacgattta tccaaggcgc
     5401 tgggcgaaaa cctgtgtcaa accactgccg ccagccgcag cagcatcgcc cggctgtcct
     5461 gcgtgtacga tgccgaacca ggctcgccgg gatttttgtc cgagctgttg cagcgcctgc
     5521 gccacgagcg cgctttcacc ctggattctt ccccgggtta ttgccgcgac tacatcaccc
     5581 tggatgacac cgtggccagc ctgatcgcca tgggcgaaca acgcgcctgc gacatcttta
     5641 acgtcgccag cggcgtcaac accttcaatc aagatattgc cgacgccctg cgccccttgg
     5701 gcgtggatat tcggttcagc cgcagcggcg aagccatgca actggcgcgc tgccatatcg
     5761 acaatctgcg cgcgctgggc gtagcgcccg ctgacaccct gcacacctta caaacccttg
     5821 tccggaacct gcccgcatga ccgcctcgct caccctgcgc cgcctggatc aggaacacct
     5881 ggcgcaattt attgcccgcc agctggagca ctttgtgccc gatggcctgc cgatcaatct
     5941 gccgttgatt cgccaacacc tgccgcaagc gctggaacgc ctgcgcgtgt gcatcaacgc
     6001 ggttcgctgg tggaaagaaa acgagttcga ccatctgcat tccagccagt actgtatttt
     6061 tttgtactac ctggccaata cgctatggaa agaaaccggc gacaccgaac tgcccaccaa
     6121 gttgtttatt ctgaacaaag cgctgaacgc cattgatctg ttttacgaga tcgacatgcc
     6181 agcgcgattc ttcattggcc actctgccgg catcgtgttg gccaaggcca gctatgcgga
     6241 ttatctggtg ctgtaccaaa actccacggt aggcaaaaac catggcgtag cgccggtaat
     6301 tgaatctggc gtgatcatgt atccgaacac cgccatcatc ggtcgctgcc atgtgcgcgc
     6361 tggcacggtg atttctcaag gggtgggcgt ggtcaaccaa gacacgcctg gcaactgtct
     6421 ggtgtttcgt ggcgtggaac agcctttgct gttcaagccc tgcgaacgca atatcctcgc
     6481 cgatatcttt cggttgtaac tcccggcgtt tcatgcgggc ccaccaccgt ggcgcccgtt
     6541 cttttttgtc ttttgctccc tcaggaatcc gctgtggccg acacttctct ggaccccctg
     6601 cgccgcgctg cgaacagcga tccgctgcat gccggcaagc gctatcaatt ggccatcagc
     6661 ttgatccaac acgcgcaagc catcagcctg gcctctcccc agtgcacaca gtggttgcag
     6721 gaagccagcg aacacctaaa ccgtattgtc gcccaagacc ctctgcacgt agacgcctgg
     6781 gctatgcttg gctacctgaa catctaccgc aatcggccag aagacgccga acccgccgtg
     6841 cgccgcgcgc tggccctgaa tcccaaccac gccgcctcac tggtgaatct gtctaatctg
     6901 caactcggcc aacgccaatt tgatgaagcg cgcgccaccc tcgaacacgc cgtgcgcgcc
     6961 accccgcaca acccttgggc gcactacaat cttgggcaag cctgtctgaa aatgggccac
     7021 agcgaagcag cgctggcgca cacacaacgc gcttttgctt tactgcccga caataaagac
     7081 attctggacg ccctgctgct gcaatcggtg tacaccgatc agatggacga ccaggagaaa
     7141 tttgccctgc accggcaata cgagcgcttg gccaatcccg acggacacac cttctcatcc
     7201 catcgaaatg accccaaccc acaacgcgcc ttgcgactgg gactggtgtc gggtgacttt
     7261 gtccatgctt cgctgatgta tgtcaccctg ccagtgctca cacgccttgc ccacatgggg
     7321 tttcaactgt ttttttatca caatggcgtc gctgtggatg cagtcacgga gcaactgcaa
     7381 gcgattgctg gcgcacaatg gcggtttacc aaggggttag acgccgacac catgtgcgcc
     7441 ttggtgcaag atgatcaaat cgacctcttg gtggacatgt caggccacgt ggagcataac
     7501 ttcctgacag cctttgcccg caaacccgcg ccagtgcaac tgagctgggc ggcttacccg
     7561 gccaccaccg ggctgagcag catcgactac gtcatcaccg atcccacgct ctggccccct
     7621 tcttctgagc agcagtactg gaccgagcag ccctgtcata tgcccgactg ctttgtggtg
     7681 taccaaccgt atttgcgcaa tccgcagcgc ggacaagacc cggcttacac cgtccgtccc
     7741 agcccagcgc tgcgccaagg ctgggtgaca ttcggcagcg gcaccagcct ggcgcgactg
     7801 accccaagca ctgtcacgct gtggacgcgt gtgctcaatg ccgtgcctga ctcacgactg
     7861 aaattggaat gcaactgctc acccgatatt gtcaaagcgc tgttcatcgc ccagggggtg
     7921 tcagcaacac gcatcgaagc cagcgaacgc acggcagaca cgcagtatct gttttatcac
     7981 gacatcgaca ttgccctgga tggcgcgccc agtaatggcg gcgtcaccag cctggacact
     8041 ttatggatgg gggtgcccct ggtcacactg cgcggaaacg ccatggcgtc ccgactgggc
     8101 gcctcgtttc tgacgcaatt aggacgaagc gactggattg ccgacagtgc ggatgaattt
     8161 gtcacgattg ccagcgcttt ggcggcagat ttacctgcac tggagcatgc ccgccagacc
     8221 ctgcggcctg ccatgcaagc gtctcccttg atgaatgctg cgcattttac cgaggccttc
     8281 agccaacgct tgcgcagtat gtggcaaaca tggtgcgcca gccctgacgc tgccactgca
     8341 cgtgatgcgc aacagcagca cgaagcgctg gcgttgtgcg aagccttgat gcaacaagac
     8401 cccgccagcg cgctgcaagc ctatcaaatg gtgctcaaag agtggccaga caatctggac
     8461 gccttgtatg ggctgggact ggccttgctg ctcaatcagc acaccgaaca agctatcacc
     8521 attctggaaa aagcccgcga ggtattctcg gcgcatggcc cggcgccagt gcatgccgag
     8581 tgtctggccg ccttgggcca agcgtggctg ttgcaaggac acctggatca agctcgcctt
     8641 tgctttcagg ccagccaaca gatctgtccc agcgctgcag tccagcaatg gttgtccgag
     8701 atcgaaaacc ctcataccct tccctctgtc tgagggcggg aagcatgtgt cctggtcagg
     8761 tttttttcga atgacggctc cggaatgcgg ttgagcatgg ccgtcagcac ctccagcgcc
     8821 gaggcggcgc agccgctgcc tagcctgacg accagcggcg gcggcatcgt aagggacagt
     8881 ggccacggcg atggcgttgc gaatctcgcg gctgatggag ctgggcgcgt gccgcaacac
     8941 ggcggagatg tgccgcaggc caagtcccct ggtgggtcat ctgcatgatg caggcgtgtt
     9001 gttcggcgca aagctggtgg tagtgcgagt ctatggcgac accttagcag agtgttgctc
     9061 ttggttcttg agactatcga tatatatata ttgatactgg cgccaggcgg cgtcatgccg
     9121 tagatatgct gggtgacatg accccccatg gcggtgttct ggtcggcatt ggcgctttgt
     9181 tgcaccgtgg cgatgtcgcg gcaggggg
//
LOCUS       DAOPYX010000106         9171 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_8912_length_9171_cov_9.275340, whole genome shotgun sequence.
ACCESSION   DAOPYX010000106 DAOPYX010000000
VERSION     DAOPYX010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 9171)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9171)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9171
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8912_length_9171_cov_9.275340"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            210..1730
                     /locus_tag="PKN55_06950"
     CDS             210..1730
                     /locus_tag="PKN55_06950"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018747389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HNX71015.1"
                     /translation="MTTIRQQDLIDSIADSLQFISYYHPKDYIQALGKAYEREESPAA
                     KDAIAQILTNSRMCAEGHRPICQDTGIVTCFVRVGMDVNWEGATMSVTDMINEGVRRA
                     YNHPDNKLRASILVDPAGARKNTKDNTPAVIHYEIVPGNSIEVDIAAKGGGSENKTKF
                     VMLNPSDSIVDWVIKTVPQMGAGWCPPGMLGIGIGGTAEKAMVLAKEALMDEIDIHEL
                     IARGPQNRVEELRIELYEKVNALGIGAQGLGGLATVLDVKIKDYPTHAASLPVAMIPN
                     CAATRHVHFVLDGSGPAQLEAPSLEDWPAVTWTPSAQARRVDLNSVTREEVASWKPGE
                     TLLLNGKLLTGRDAAHKRMTDMLNQGEKLPVDFTNRFIYYVGPVDPVRDEVVGPAGPT
                     TATRMDKFTRQMLAETGLLGMVGKSERGPTAISAIKDHKAVYLMAVGGAAYLVSKAIK
                     ASRVVAFADLGMEAIYEFDVVDMPVTVAVDSGGTSVHETGPKEWQAKIGKIPVVQG"
     gene            2076..3794
                     /locus_tag="PKN55_06955"
     CDS             2076..3794
                     /locus_tag="PKN55_06955"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNX71016.1"
                     /translation="MAIYRQIERTFFNNLTKKLLSVFMVFIPHAALTAYLSFKLDDPV
                     LNWLVWSALAVLTVATLCWVWYLRHLTVTPLREIITLLNQHSEGTGDLSHDVPVYTHD
                     EIRELATSHNRFQGKLRDMINRIRQLAIHTAVESARLVQMSRQVSGHAQSQRTLSESV
                     RSLSAATASDVEAMSGETGAIAGQTENNLHLARESHGELLDITRHIHDMSQSMQRFSR
                     TVSELSQQSVEIQSIAGLIKDVAEQTNLLALNAAIEAARAGEAGRGFAVVADEVRKLA
                     ERVGRATEDIFANIDHMVGQVDETLLETQGIVRTTEITKQVVDRASGNFSSLVADFEQ
                     NSARLARMADTMQALAARNREISTHVAQVHTLSADITQAVGAVEKTTRDMNAAAENVQ
                     EVVGSFTTGDGVVEQWVAKLGEFRARAQQELQAIAAQGLDVFDQRYQPVAGSAPPKFR
                     LAYTDAFQQRIQPLLDEIARAMPQCVATCLVDTQGYAPTHQSRFAQPQSGELSQDIQH
                     SRHMRRFDDPIGLRSARNTKPFLVQTYSRDTGEVLSEISLPVMVDGRHWGALRMAVST
                     DAMLAQ"
     gene            complement(3808..4266)
                     /locus_tag="PKN55_06960"
     CDS             complement(3808..4266)
                     /locus_tag="PKN55_06960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011137020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2721 domain-containing protein"
                     /protein_id="HNX71017.1"
                     /translation="MHIEINTPALLFPAISLLLLAYTNRFLGLSSIIRSLHHEYLQDP
                     DPKIIGQLDNLRRRVALIRGMQIVGTLSIFCCVVSMVLVFIGQGEIATWVFGASLLLM
                     SVSLAMSVHELFISGDALNILLTDMAEPLAAHQQRDAQPRRRRAPEDDAP"
     gene            complement(4320..4868)
                     /locus_tag="PKN55_06965"
     CDS             complement(4320..4868)
                     /locus_tag="PKN55_06965"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HNX71018.1"
                     /translation="MKMVNWLSRWSLLLSLLAAISLPVWASGPAQGEAAPAFRLADQH
                     GVLRDLSEFRGRWLVLYFYPKDQTQGCTEQACSFRDDFVKLKLLGAEVVGISLDSKES
                     HAAFAQKYLLPFPLLSDPDGEVAARYGALTNLLVLKFAKRHTFLIDPQGKVARRYTDL
                     DTATYASRIVKDLSTLTRKPAH"
     gene            complement(4855..5502)
                     /locus_tag="PKN55_06970"
     CDS             complement(4855..5502)
                     /locus_tag="PKN55_06970"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014086205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HNX71019.1"
                     /translation="MRAAMMAALLWTLPAASMAAPDAQAWAQWQQQQQQQTLHEAYPN
                     PRTPGQPWVKVGVRSQTLSVFSAEGTLEKRYDISTAKRGVGETQNSYQTPRGWHRICE
                     KIGDGAASDTIIFRREVTPWKYTPELHAEYPNKDWILTRILWLCGQEPGLNQGSRDGV
                     LVDSYARYIYIHGAGRHVPMGTPTSLGCVRMTSEAVIELFERTPVGMEVLIDENG"
     gene            complement(5487..5999)
                     /locus_tag="PKN55_06975"
     CDS             complement(5487..5999)
                     /locus_tag="PKN55_06975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012697983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HNX71020.1"
                     /translation="MHIVIELDRQRLRLLAEDGALLREYPVSTAARGAGEQRGSYQTP
                     RGRHVIRAKIGAGAPRGAVFVGRRWTGECCTPESWAAKPDRDWILTRILWLSGCEVGL
                     NRRGDVDTMRRYIYIHGTPDAVELGKPGSHGCVRMNNDDLIELFDRVPAGTPVNIVEH
                     SQKESACAQQ"
     gene            complement(5999..6736)
                     /gene="tadA"
                     /locus_tag="PKN55_06980"
     CDS             complement(5999..6736)
                     /gene="tadA"
                     /locus_tag="PKN55_06980"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009118384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HNX71021.1"
                     /translation="MTAADPLARLGPKTQAWLAELGVHSHAQLIELGAVEAFVQLKAR
                     GHTVARTLLWALHGCVLGCRGSELDAELRESLWQAARARPTPPPPLDWAEAERWMALA
                     LALAREAAEAGEVPVGALVVKDGQVIGRGFNQPIGRHDPSAHAEMLALRQAAATLGNY
                     RLDGCSLYVTLEPCPMCAGAIQHARIARLVFGAHDGKTGAAGSVVNLFAEPRLNHHTA
                     VYPGVRAAEAGALLSDFFRARRARSPA"
     gene            complement(6876..7745)
                     /locus_tag="PKN55_06985"
     CDS             complement(6876..7745)
                     /locus_tag="PKN55_06985"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF013222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HNX71022.1"
                     /translation="MAENSLPSPSASNPVTRACRLLEHAEPTPSLSELAQAVGKSVSQ
                     LHRLFKAQLGLTPRQYALAQRAARLRAQLPTARTVTEAMHEAGFASSGRFYAHTDAML
                     GMTPGRYRAGGAHEQLRVAVAQTPLGALLVASSARGIAAMALGDDPEALLRDLQDRFP
                     RAELLAGDADYEALVAQVVGWMARPGADLGLPLDIRGTAFQQRVWQALRDIPSGHTVS
                     YRELAARIGAPRAVRAVASACAANPLAVVIPCHRVVRSNGELAGYAWGIERKRALLAH
                     EAASAPGEGDTPA"
     gene            complement(7869..8741)
                     /gene="fdhE"
                     /locus_tag="PKN55_06990"
     CDS             complement(7869..8741)
                     /gene="fdhE"
                     /locus_tag="PKN55_06990"
                     /inference="COORDINATES: protein motif:HMM:NF016131.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HNX71023.1"
                     /translation="MSVDLLNAAEPDATLAPDAAVFATRAARFAQLANGHALGPWLAF
                     LGQLSLAQSQLRALPPAPPATATQPLWPATAASLPAEWPRVLHELGALLSQDAPPPVR
                     AAIEALASASPAQLHALAEQVLADARGQGGAPSASDAAERLLVAAALQACWTHWARQT
                     PVKLAASASHCPCCGGAPVASVIQIGARLGKLRYLSCTLCNTRWYAVRARCTACDSDG
                     KIHYHQLEDGPAGVRAETCDACQSYLKILFLEQDPALDPVADDLASLALDVALGEAGH
                     PRNGPNLFLLNSKA"
     gene            complement(8816..>9171)
                     /locus_tag="PKN55_06995"
     CDS             complement(8816..>9171)
                     /locus_tag="PKN55_06995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012696290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HNX71024.1"
                     /translation="STGAAHAAAWVNLAGDIELAREDVGRHNALDKLIGARAKAGADN
                     RDGFALVSSRASYEMVQKSALAGIGALAAMSAPTALAVRLAEETGVSLVGFARQHSLV
                     VYAGGEYVHMDIDPS"
BASE COUNT         1689 a         2934 c         2948 g         1600 t
ORIGIN      
        1 ggacaccgcc ctttcacggc gataaccggg gttcgaatcc ccgtggggac gccaaacaag
       61 caaaaaagcc aggctgatgc ctggcttttt tgcgttcttg tcgttttctt tcgattttac
      121 tgcaacgcaa tatcccggtt gggcgcaggt gcgagtaaaa tgccgcctgc tgatatgtcc
      181 cccataatcc aaaatcagag gagcgttgca tgaccaccat ccgccagcag gatctgatcg
      241 acagcattgc cgacagcttg cagttcatct cttactacca cccgaaggac tacatccagg
      301 ccttgggcaa agcgtatgag cgcgaagaaa gcccggccgc caaggatgca atcgctcaga
      361 ttctgaccaa cagccggatg tgcgccgaag gccaccgtcc gatttgccag gataccggca
      421 tcgtcacctg ttttgtgcgc gtgggcatgg acgtgaactg ggaaggcgcc accatgagcg
      481 tcaccgacat gatcaacgaa ggcgtgcgcc gcgcttataa ccatccggac aacaagctgc
      541 gcgcctccat cctggttgac ccggccggtg cccgcaagaa taccaaggac aacaccccgg
      601 cggtgattca ctacgaaatc gtgccgggca acagcatcga agtggacatc gccgccaagg
      661 gcggtggttc ggaaaacaaa accaagttcg tcatgctcaa cccgtctgac agcattgtcg
      721 actgggtgat caaaaccgtg ccgcaaatgg gcgctggctg gtgtccgccg ggcatgctgg
      781 gcattggcat tggcggcacc gccgaaaagg ccatggtgct ggccaaagaa gcgctgatgg
      841 acgaaatcga catccacgaa ctgattgccc gtggcccgca aaaccgcgtg gaagaactgc
      901 gcattgagct gtacgaaaag gtcaacgcgc tgggcattgg cgcccaaggc ctgggcggcc
      961 tggccaccgt gctggacgtg aagatcaagg actacccgac ccacgccgcc agcctgccgg
     1021 tggcgatgat ccccaactgc gccgccaccc gccatgtgca ctttgtgctg gatggctccg
     1081 gcccggccca gctggaagcg ccgagcctgg aagactggcc ggccgtcacc tggaccccgt
     1141 cggcccaggc ccgtcgtgtg gatctgaaca gtgtcacccg cgaagaagtg gccagctgga
     1201 agccgggcga aaccctgctg ctgaacggca agctgctgac tggccgcgac gccgcgcaca
     1261 agcgcatgac cgatatgctg aaccagggcg aaaagctgcc ggtggacttc accaaccgct
     1321 ttatctacta cgtgggcccg gttgacccgg tgcgtgacga agtcgtcggc ccggctggcc
     1381 cgaccaccgc cacccgcatg gacaaattca cccgccagat gctggccgag accggcctct
     1441 tgggcatggt gggcaaatcc gagcgtggcc cgaccgccat cagcgccatc aaggaccaca
     1501 aggccgtgta cctgatggcg gtgggcggcg cggcgtatct ggtgtccaag gcgattaaag
     1561 cctcgcgcgt ggtggcgttt gccgacctgg gcatggaagc catttacgaa tttgacgtgg
     1621 tggacatgcc ggtgactgtg gcggtggatt cgggcggcac ctcggtgcac gaaaccggcc
     1681 cgaaagaatg gcaagccaag atcggcaaga ttccggttgt gcaaggctga ttgcgaccgc
     1741 tgaccatgtt ttgtgtgccc ttgccagacg atttgtcctg ttaatggcca cacacctgat
     1801 tcagcatcgc tgcgctgagt tttgtgggca gcgcttaatc tgaaacccgc cgggtcaagc
     1861 ccggcgggtt tatttttggc ataagcaaca ggttcattat acggtaggaa tatcagtata
     1921 cattcgaacg tttgcctttc cggggtgtcc atggtctggt ctgctgcgtg ttggcgcggc
     1981 gattgcttga tgccagaacc cgacgcccaa tgtttccggg atggcaaaaa caggggcgca
     2041 gtatgggaaa gacgctattt caacgtttgg aaaatatggc tatctaccgt caaatcgaac
     2101 gcacgttttt caacaacctg accaaaaaac tgctcagcgt gttcatggtg tttattccac
     2161 acgcagcatt gaccgcatat ttgtcgttca agctggatga tcccgtcttg aattggctgg
     2221 tctggtccgc cttggccgtg ctgacggtgg ctaccctatg ctgggtgtgg tatctgcgcc
     2281 acctgactgt caccccgctg cgcgagatca tcaccttgct gaatcaacac agcgagggca
     2341 cgggcgattt gtcgcacgac gtgccggtat acacccacga cgaaatccgc gagctggcca
     2401 ccagccacaa tcgtttccag ggcaagctgc gcgacatgat caaccgcatc cgccaattgg
     2461 ccatccacac cgctgtcgaa tcggccaggc tggtgcaaat gagccgccag gtgtccggcc
     2521 atgcgcaaag tcagcgcacg ctgagcgaat cagtgcgcag cctgagcgcc gccaccgcca
     2581 gcgatgtgga agccatgtcg ggcgaaaccg gcgccattgc cgggcaaacc gaaaacaatc
     2641 tgcacttggc gcgtgaatcg catggcgagc tgctggacat cacccgccat atccacgaca
     2701 tgagccagtc catgcagcgc ttttcccgca cggtgagcga gctgtcgcag caatcggtcg
     2761 aaatccagtc gattgccggc ctgattaagg acgtggccga gcaaaccaat ctgctggcgc
     2821 tgaacgccgc catcgaagct gcgcgcgctg gtgaggccgg gcgcggtttt gccgtggtgg
     2881 ccgatgaagt gcgcaagctg gccgagcgag tgggccgcgc cacggaagac atttttgcca
     2941 atatcgacca tatggttggc caggtggacg aaaccttgct ggaaacccag ggcattgtgc
     3001 gcaccaccga aatcaccaag caggtggttg atcgcgcctc gggcaatttc tcgtcgctgg
     3061 tggcggactt cgagcagaac agcgcgcgtc tggcgcgcat ggccgacacc atgcaggcgc
     3121 tggcggcgcg caaccgcgag atttccaccc atgtcgccca agtgcacacc ctgtctgccg
     3181 acattaccca ggcggtgggc gccgtggaaa aaaccacccg cgacatgaac gccgccgccg
     3241 aaaacgtgca ggaagtggtc ggctcgttca ccaccgggga tggcgtggtc gagcaatggg
     3301 tggccaaact cggggagttt cgcgcgcgcg cccagcagga attgcaggcc atcgccgcgc
     3361 aggggctgga tgtgtttgac cagcgctacc agccggtggc cggcagcgcg ccgcccaagt
     3421 tccgcctggc ctacaccgac gccttccagc agcgcattca gcccttgctg gacgagattg
     3481 cccgcgccat gccgcaatgt gtcgccacct gcctggtgga cacccaaggc tatgcgccga
     3541 ctcaccagag ccgctttgcc cagccgcaaa gcggtgagtt gagccaggat attcagcaca
     3601 gccggcatat gcgccgcttt gacgacccca ttggcctgcg ctcggcgcgc aacaccaagc
     3661 ctttcctggt gcagacctat tcgcgcgaca ccggtgaggt gctgtcggaa atctcactgc
     3721 cagtgatggt tgatggccgg cattggggcg cgttgcgcat ggcggtgtcc accgacgcca
     3781 tgctggcgca gtgagcaagc ggcagggcta gggcgcgtcg tcttctggcg ctcggcggcg
     3841 gcgcggttgg gcatcgcgct gctgatgggc ggccagcggc tcggccatgt cggtgagcag
     3901 aatattcagc gcgtcgcccg agatgaacag ctcgtgcacc gacatcgcca aagacacact
     3961 catcagcagc aggctggcgc caaacaccca ggtggcgatt tcaccctggc caatgaacac
     4021 cagcaccatg ctgaccacgc agcaaaaaat gctgagcgtg ccgacaatct gcatgccgcg
     4081 aatcagcgcc acccgtcggc gcagattatc cagttggcca atgattttcg ggtcggggtc
     4141 ttgcagatac tcatggtgca gcgagcgaat gatgctggac aggcccagaa agcggttggt
     4201 gtaggccagc aacagcaggg agatggccgg aaacagcaag gccggggtgt tgatttcgat
     4261 atgcatggga gcagtcagca gcagcgccgg tgtgcatgcc ggcgctggaa gtcatatggt
     4321 cagtgagccg gcttgcgcgt cagcgtgctc aggtccttga cgatgcggct ggcgtaggtg
     4381 gcggtgtcca ggtcggtgta gcggcgagcc accttgcctt gcgggtcgat caaaaaggta
     4441 tgacgcttgg cgaatttcag caccagcaag ttggtgagcg cgccatagcg ggcggctacc
     4501 tcgccatccg ggtcgctcag cagcgggaag ggcagcaggt atttctgggc aaaggccgcg
     4561 tggctttcct tgctgtccag gctgatgccg acgacttcag cccccaggag cttgagtttg
     4621 acaaagtcat cgcgaaaact gcatgcctgt tcggtgcagc cttgggtttg gtctttcggg
     4681 taaaaataca gcaccagcca gcggccacga aactcgctca gatcgcgtag caccccgtgt
     4741 tgatctgcca gccggaaggc cggcgcagct tcgccttgtg ccgggccact ggcccaaacc
     4801 ggcaggctga tcgctgcgag caagcttaac aacaggctcc agcgcgacag ccaattaacc
     4861 attttcatca atcaaaacct ccatgccgac aggcgtgcgc tcgaacaatt caatcaccgc
     4921 ctcgctggtc atgcgcacac agcccagtga agttggcgtg cccatcggca catggcggcc
     4981 ggcgccgtgg atgtaaatat agcgggcgta actgtctacc agcaccccat cgcggctgcc
     5041 ctggttcagt cctggctcct ggccgcacag ccagaggatg cgggtcaata tccagtcttt
     5101 gttgggatat tcggcgtgga gttccggggt atatttccag ggggtgactt cccggcgaaa
     5161 aataatggtg tcactcgctg cgccatcgcc gattttttcg catatccgat gccagccacg
     5221 cggggtttgg tagctgtttt gtgtttcgcc caccccgcgc ttggcggtgg aaatgtcgta
     5281 gcgtttttcc agtgtgcctt cggctgaaaa cacactcagc gtctgactgc gcacgcccac
     5341 cttgacccac ggttgtcctg gggtgcgcgg gttggggtac gcctcgtgca aggtctgctg
     5401 ctgttgttgc tgttgccact gcgcccaggc ttgggcatcg ggcgcggcca tggatgctgc
     5461 cggcagcgtc cataacagcg cggccatcat tgctgcgcgc atgcgctctc cttctggctg
     5521 tgttcgacaa tattcaccgg cgtgccggca ggaacccgat caaacagttc gatcaggtcg
     5581 tcgttattca tgcgcacgca gccatgcgag ccgggcttgc ccagctcaac cgcatctggc
     5641 gtgccgtgga tatagatata gcggcgcatg gtgtccacgt ctccccgccg attaagcccc
     5701 acttcgcagc cagacagcca caaaatccgg gtcagaatcc agtcgcggtc gggcttggcc
     5761 gcccagcttt ctggcgtgca gcattccccg gtccagcgcc ggccgacaaa caccgcgccg
     5821 cgcggcgcgc cagcgccaat cttggcgcgg atcacatgcc ggccgcgtgg ggtctggtag
     5881 ctgccgcgct gctcgccagc gccgcgcgcg gcggtggaca ccgggtattc gcgcaacagc
     5941 gcgccatctt cagccagcag ccgcaggcgc tggcggtcta gctcaatcac gatgtgcatc
     6001 aggcagggct ccgggcgcgg cgggcgcgaa aaaaatcgga caatagcgca cccgcctcgg
     6061 cggcgcgcac gccagggtac acggcggtgt gatgattcag acgcggctcg gcaaacaagt
     6121 tcaccacgct gccggcggcg ccggttttgc cgtcgtgcgc gccaaacacc agccgggcga
     6181 tgcgcgcatg ctggatggcg ccggcgcaca tcgggcacgg ctccagcgtc acatacaggc
     6241 tgcatccgtc cagccggtaa ttgcccagcg tggccgccgc ctggcgcaag gccagcattt
     6301 cggcgtgggc gctggggtcg tggcggccaa tcggctgatt aaagccacgg ccaatcacct
     6361 ggccatcttt caccaccagc gcgcccaccg gcacttcgcc tgcttcagcc gcctcgcgcg
     6421 ccagcgccag cgccaacgcc atccagcgtt cggcctcggc ccaatccagc ggcggcggcg
     6481 gtgtcggccg cgcgcgcgcc gcttgccaca gcgactcgcg cagctcggcg tcgagttcgc
     6541 tgccacggca gcccaacacg cagccatgca atgcccatag caaagtgcgc gccaccgtat
     6601 ggccgcgcgc cttgagctgc acaaacgcct ccactgcgcc cagctcaatc aactgggcgt
     6661 gactgtgcac gcccagctcg gccagccagg cttgggtttt tggcccgagg cgggccagcg
     6721 ggtcggcggc cgtcatcgac ggcctttccg gcaaatgggg aatagacaga aaaacacagg
     6781 catgtcgtta ttttagtgcg ccagcgcggg ccggtcacgc aacatgcgta ggaatacgga
     6841 gcagacctgc ctccgtgggt gggcgatggc gggggtcagg cgggtgtgtc gccctcaccc
     6901 ggcgcgctcg ccgcctcatg cgccagcaag gcgcgcttgc gctcgatgcc ccaggcgtag
     6961 ccggccagct cgccattgct gcgcaccacc cgatggcagg gaatcaccac cgccagcgga
     7021 ttggcggcgc aggcgctggc caccgcgcgc accgcgcgcg gcgcgccaat gcgcgcggcc
     7081 aactcacggt agctgacggt gtgcccgctg ggaatatcgc gcagcgcctg ccacacccgc
     7141 tgctgaaaag ccgtgccgcg aatatccagc ggcaagccca gatcagcgcc ggggcgcgcc
     7201 atccagccga ccacctgcgc caccagggct tcgtagtccg cgtcgcccgc caacagctcg
     7261 gcgcgcggaa agcggtcttg tagatcgcgc agcagcgctt ctgggtcgtc gcccagcgcc
     7321 atggcggcga tgccgcgcgc gctggacgcc accagcaagg cgcccaaggg ggtttgcgcc
     7381 accgccacgc gcagctgctc atgcgcgccg cccgcccgat agcggcctgg cgtcatgccc
     7441 agcatggcgt cggtgtgggc ataaaaacgc ccgctggagg caaacccagc ctcatgcatg
     7501 gcctcggtca ccgtgcgcgc ggtgggcaac tgcgcgcgca gccgcgccgc gcgttgcgcc
     7561 aaggcatatt gccgcggcgt cagccccagc tgcgccttga acaggcgatg caactggctg
     7621 acgctcttgc ccaccgcctg cgccaactcg ctcaggctgg gcgtgggctc ggcgtgctcc
     7681 aacaaccggc aggcgcgggt gaccgggttg cttgcgctgg gggaaggaag tgagttctcg
     7741 gccatggggg gtctttcatc aagcggtggg tcaagcctga ccgactgtag cccgctggcc
     7801 gagaaaaaac actccgattc ttgtcatctc atcttcactc ccaccttgtc cggtctgggc
     7861 tggcgggttc aggccttgct gttcagcaaa aacagattcg gcccattgcg cgggtggccg
     7921 gcttcgccca gcgccacgtc cagcgccagg ctggccaggt cgtcggctac tgggtccagc
     7981 gccgggtctt gctccaggaa cagaattttc agatagctct gacaggcgtc gcaggtttcc
     8041 gcgcgcacgc cggccgggcc gtcttccagc tgatggtagt gaatcttgcc gtctgaatca
     8101 caggcggtgc agcgcgcacg cacggcgtac cagcgggtgt tgcacagcgt gcagctcagg
     8161 tagcgcagct tgcccagccg ggcgccgatc tggatcacgc tggcgactgg cgcgccgcca
     8221 cagcaagggc agtggctggc gctggcggca agcttgaccg gggtttggcg cgcccagtgc
     8281 gtccagcagg cttgcagcgc ggcggccacc agcaggcgct cggcggcgtc gctggcgctg
     8341 ggcgcgccgc cctggccgcg cgcatccgcc agtacctgtt cggccagcgc atgcagctgc
     8401 gccgggctgg cgctggccag cgcctcgatg gcggcgcgca ctggcggcgg cgcgtcctgg
     8461 ctgagcaggg cgcccagttc gtgcagcacg cgcggccact cggccggcag gctggcggcg
     8521 gtggccggcc acagcggctg ggtggcggtg gccggcggcg ctggcggcaa ggcgcgcaac
     8581 tgcgattgcg ccaggctgag ctggcccaaa aacgccagcc aagggcccag cgcgtggcca
     8641 ttggccagtt gggcaaagcg cgcggcgcgg gtggcgaata cagcggcgtc tggcgccagg
     8701 gtggcgtccg gttcggcggc gttgagcaga tcaacagaca tgggggtttt ccagaatcag
     8761 gcgcgcctgc cgccagggca ggcgcgtaat atcggtgaag gggagggcgc ggcgttcagc
     8821 ttgggtcgat atccatgtgg acatattcgc cgccagcgta caccaccagg ctgtgctgac
     8881 gggcaaagcc caccaggctg acgccggttt cttccgccag gcgcaccgcc agcgcggtgg
     8941 gggcggacat cgccgccagc gcgccgatgc cggccagggc ggatttttgc accatttcgt
     9001 agctggcgcg gctggacacc agggcaaagc cgtcgcggtt atcggcgccg gccttggcgc
     9061 gggcgccaat cagtttatcc agcgcattgt ggcggccaac gtcttcgcgc gccagttcga
     9121 tgtcgccggc cagattcacc caggcggcgg cgtgcgcggc gccggtgctc t
//
LOCUS       DAOPYX010000107         9135 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_8969_length_9135_cov_12.214648, whole genome shotgun sequence.
ACCESSION   DAOPYX010000107 DAOPYX010000000
VERSION     DAOPYX010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 9135)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9135)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9135
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8969_length_9135_cov_12.214648"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(16..945)
                     /locus_tag="PKN55_07000"
     CDS             complement(16..945)
                     /locus_tag="PKN55_07000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019923812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HNX71025.1"
                     /translation="MLITLLQALAPIALLIALGAGLRRRAVLSDAFWPQAERLSYYVL
                     LPALFIHGLASARLDGLPVWPMVAALVASTVAVALGLWAARPHLAVSRPAFTSVFQGG
                     VRFNNYVGVSAAAGLFGAQGVALAAVANAAIVPTVNILCVLVFARYGHGGDASARGIA
                     RQLLANPLVVACAIGIGLQAGGVTLPTLLAHTLHTLGQASLAIGLLCVGAALDFSTAR
                     RWLRPVGQASLVKFLVMPLATLAACDAFGLHGAAAGAALLFQSLPTASSSYIMARQLG
                     GDAPLMAGIIASQTVLAGLTLPAVMLAHAQLWP"
     gene            complement(1090..1989)
                     /locus_tag="PKN55_07005"
     CDS             complement(1090..1989)
                     /locus_tag="PKN55_07005"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HNX71026.1"
                     /translation="MSLRALRTLAAIAQHGSFARAAEAVCLTQSAVSLHVRTLEEAFN
                     APLFDRSRRTPTLTEAGRRAVERAREILAIYDGIAGELGEGGELAGRLRIGAIQTALA
                     GVLPAALAALRRQHPRLRVQVYSGMSAELASRLDAGELDAAVTTEPVRPHPPGLISTP
                     LYQDGFWVIAPPGLHAQDARTLLQQHPFIRFDRRSWAGRVIDSELRRQRWQVHTEMEL
                     DSQDAIVQMVACGLGVAVAPLSREVRARLDGLGLTCLPFAEPQRLRRVVLLEQVEQPA
                     ARLSAALAEAIRQQAGDEAIHEK"
     gene            complement(2045..2569)
                     /locus_tag="PKN55_07010"
     CDS             complement(2045..2569)
                     /locus_tag="PKN55_07010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019959380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNX71027.1"
                     /translation="MNFLLIDDDEVFASVLRRALSRRGYCAHWAADGEAALAALKETA
                     PSHIVLDLNLGGESGLRLLPHLREQAPQAVILVLTGYASIATAVDAIKLGAAQYLAKP
                     AGVDDILAAFEQREPNPAQHAPEHTMSVRRLTWEHIQRVLAEHDGNVSATARALDMHR
                     RTLQRMLAKKPANR"
     gene            complement(2566..3807)
                     /locus_tag="PKN55_07015"
     CDS             complement(2566..3807)
                     /locus_tag="PKN55_07015"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNX71028.1"
                     /translation="MPAESCTVALSRLLALNWGFILLASLGLLVAAQDGMALPWPPML
                     ATLATLALGNMLAALWTRRASNVSPWVLAVALALDCTALGVLLFFSGGANNPLVTLYL
                     LPLVYAALTLPRRQAWWLAVLAVADYTFLMDFFIPLPGSHSDPALAFRLHTVGMWATF
                     CGAAALMVGLIGKMTAELRARDRALRDFREKQLRDEQWVALGTLAASAAHELGTPLST
                     LAVLIGEWRRAPLDNPDLHEDLDLMHTQVDACKAALNRLASEAGVARAASRQAADAYM
                     AQLLRQWRTLRPDVAVRYQPDPAAAPQLALDAGVTAALLNLLNNAADASPQEVDIHTN
                     WSGEAFCLTIRNRGPGLSPSLLAAAGTPGAPSSKGGMGLGVALAHATFERLGGEVRLR
                     NRAGGGVEATVILPWPKENAS"
     gene            complement(4371..4763)
                     /locus_tag="PKN55_07020"
     CDS             complement(4371..4763)
                     /locus_tag="PKN55_07020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein FimV"
                     /protein_id="HNX71029.1"
                     /translation="MVDLITESNTLIPLALFASLSLYWMWRRRQTRLSIQRARRIDPI
                     GEAEVFLAYGRSKDALRVLQTALNREPGNVKIKVVMLRALAEQQDAHGFVRLAGEVRG
                     ALHGQPVWRQICKTGQQLQPGHPLFQLA"
     gene            complement(5373..6464)
                     /gene="aroB"
                     /locus_tag="PKN55_07025"
     CDS             complement(5373..6464)
                     /gene="aroB"
                     /locus_tag="PKN55_07025"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008952255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HNX71030.1"
                     /translation="MITLDLVLPDTRYPIYIGESLYADAARIVAHLPQKKAAIITNET
                     IAPLYLAPLQAALEAAGVAVTPVILPDGEAYKTWETLNLIFDALLGARAERKTTLIAL
                     GGGVIGDMTGFAAACYQRGAPFIQIPTTLLAQVDSSVGGKTAINHPLGKNMIGAFYQP
                     RAVLADMAVLDTLPERELSAGLAEVIKYALLGDVQFLAWLEQHIEALRARDRATLAEA
                     VRHCCQMKADIVGEDEKETGVRALLNLGHTFGHAIEAGMGYGAWLHGEAVAAGMVLAA
                     ETSRLLGWISEADVARVTALIERAGLPILSPDLGVDAWISHMGHDKKVEEGQLRFVLL
                     RQLGQAVIEKGVPLAVLREVLQGRFIRRA"
     gene            complement(6451..7023)
                     /gene="aroK"
                     /locus_tag="PKN55_07030"
     CDS             complement(6451..7023)
                     /gene="aroK"
                     /locus_tag="PKN55_07030"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012698399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HNX71031.1"
                     /translation="MIHVAGNLFLVGLMGAGKTTVGRTLARETGKSFYDSDHEIEARA
                     GVRVATIFEIEGEPAFRERERKTIAELAERQNIVLATGGGAVTHPDTRRVLAANGTVI
                     YLRASIDDLLLRTQNDKNRPLLQTADPRAKLESLFAQRDPLYREVADIIIDTSRQNVH
                     LLVSRLLQKLAHHSPCPSTPLPGASPHDHA"
     gene            7206..8399
                     /gene="dapC"
                     /locus_tag="PKN55_07035"
     CDS             7206..8399
                     /gene="dapC"
                     /locus_tag="PKN55_07035"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014088724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009016 - succinyldiaminopimelate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0019877 - diaminopimelate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyldiaminopimelate transaminase"
                     /protein_id="HNX71032.1"
                     /translation="MNPNLSLLHPYPFQKLRELFLGVVPNPDLKPVNLSIGEPKHATP
                     TLVTEALTANLGGLAQYPATQGSEALRQAAARWLSRRYGLRGLDIQREILPVNGSREA
                     LFAFVQAVVDASGAEKPVVISPNPFYQIYEGAALLAGAEPYYLNCLADQRFQPDWASV
                     PAEVWARTQLVFVCSPGNPTGSVMSLNDWQDLFELSDRYGFVIASDECYSEIYFDAPP
                     LGGLEAAATLGRDDFANLVMFTSLSKRSNVPGMRSGFVAGDARVLEKFLLYRTYHGSA
                     MSPAIQAASVAAWDDEAHVEHNRQLYAEKFAAVTPVVAQALDVSLPEASFYLWAKVPG
                     DDAEFARELYREQHVTVLPGSYLARDAHGVNPGAGYIRIALVAPLAECQEAAERLVTF
                     ARSRA"
     gene            8519..>9135
                     /locus_tag="PKN55_07040"
     CDS             8519..>9135
                     /locus_tag="PKN55_07040"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011134005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HNX71033.1"
                     /translation="MHPIQTLIEESFEKRADITPATASPELKSAVMQVIDELDNGRIR
                     VAEKHGDDWVVNQWVKKAVLLSFRIRDNAPMAGGCMQYYDKVDTKFADYTEREFREGG
                     FRVVPNAVARRGSFIGKNVVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVGEGSVISMGV"
BASE COUNT         1660 a         2896 c         2977 g         1602 t
ORIGIN      
        1 catgacgtgc gatgctcacg gccacagctg ggcgtgcgcc agcatcaccg ccggcagcgt
       61 caggccggcc agcacagtct ggctggcgat gatgccggcc atcagcggtg catcgccgcc
      121 cagctggcga gccatgatgt aggacgacga ggcggtgggc aggctctgga acaacagcgc
      181 tgcgcctgct gccgcgccgt gcaggccaaa ggcgtcgcag gcggccagcg tggccagcgg
      241 catcaccaga aacttgacca gcgaggcctg acccaccggg cgcagccagc gccgggcggt
      301 ggaaaaatcc agcgccgcgc ccacgcacag caggccaatc gccaacgagg cctggcccag
      361 cgtgtgcagg gtgtgcgcca gcagggtggg caaggtcacg ccgccggctt gcaggccgat
      421 gccgatggcg caggccacca ccagcggatt ggccagcaat tgccgggcaa tgccgcgcgc
      481 cgaggcgtcg ccgccgtggc cgtagcgggc aaacaccagc acgcacagga tattcaccgt
      541 gggcacaatc gccgcattgg ccaccgccgc cagcgccacg ccctgcgcgc caaacagccc
      601 ggcggcggcg gatacgccga cataattgtt aaagcgcacc ccgccctgaa acaccgaggt
      661 gaacgccggc cggctgaccg ccagatgcgg gcgcgccgcc cacagcccca gcgccaccgc
      721 cacggtggac gccaccagcg ccgccaccat cggccacacc ggcaacccgt ccagccgcgc
      781 gctggccagg ccgtggatga acagcgccgg caacagcacg taataactca gccgctccgc
      841 ctgcggccag aacgcgtcgc tgagcacggc gcggcggcgc aagcccgcgc ccagggcaat
      901 cagcaaggca atcggcgcca gcgcctgcaa caaggtgatc aacatggcgc acctgcaacg
      961 ggcacgcggc gacaaacatg ggaaagattc aacacagaca tcgggcaaac ccggcaggca
     1021 acaagaaggc ccagaagata cctggctttt gcttgagaaa aaatcgagtt taattgcagc
     1081 taaacccaat tatttctcat ggatggcctc gtcgccggcc tgctggcgga tcgcctcggc
     1141 caaggcggcg ctcaaacgcg ccgccggttg ctcgacttgc tccagcagca ccacccggcg
     1201 caggcgttgc ggctcggcaa acggcagaca ggtcagcccc agcccatcca gccgcgcgcg
     1261 cacctcacgc gacagcggcg ccaccgccac gcccagcccg caggccacca tctgcacaat
     1321 ggcgtcctgg ctgtccagct ccatttcggt gtgcacctgc cagcgctgcc ggcgcaattc
     1381 gctgtcgatc acccgcccgg cccaggaacg acggtcaaag cggataaacg ggtgttgctg
     1441 caagagcgtg cgcgcgtcct gcgcgtgcaa acccggcggg gcaatcaccc aaaaaccatc
     1501 ctgatacaag ggcgtgctga tcaagcccgg cgggtgcggg cgcaccggct cggtggtcac
     1561 cgcggcgtcc agctcgccag cgtccagccg gctggccagc tcggccgaca tgccggaata
     1621 cacctgcacg cgcaggcgcg gatgctggcg gcgcaaggcg gccaaggctg ccggcaacac
     1681 cccggccagt gcggtctgaa tggcgccgat gcgcaaacgc ccggccagtt cgccgccctc
     1741 gcccaactcg ccggcaatgc catcgtaaat ggccagaatc tcgcgcgcgc gctccaccgc
     1801 ccgccgcccg gcctcggtca gcgttggcgt gcgccgcgag cggtcgaaca aaggggcgtt
     1861 gaaggcttct tccagggtgc gcacatgcag gctgaccgcc gattgggtca ggcataccgc
     1921 ttcagcggcg cgggcaaacg aaccatgctg ggcaatcgcc gccagcgtgc gcagggcgcg
     1981 caaggacatt ggataagggg ctccatgttg tgagcggtgt ttaacgtcgc tggatatcac
     2041 cggctcagcg gttggccggt tttttggcca gcatgcgttg cagcgtgcgc cgatgcatat
     2101 ccagcgcgcg ggcggtggcc gagacattgc catcatgctc ggccagcacc cgctggatgt
     2161 gctcccaggt gagccggcgc accgacatgg tgtgctcggg cgcgtgttgc gccgggttgg
     2221 gctcgcgctg ctcaaacgcg gccaggatgt catctacccc ggcgggcttg gccaggtatt
     2281 gcgccgcgcc cagtttgatg gcatccacgg cggtggcgat gctggcgtag ccggtcagca
     2341 ccagaatcac cgcctgtggc gcctgctcgc gcaagtgcgg cagcaggcgc aggccacttt
     2401 cgccgcccag gttcaggtcc agcacaatat ggctgggggc ggtttctttg agggcggcca
     2461 gcgctgcttc accatcggcc gcccagtgtg cgcagtagcc acgccggctt agggcgcgac
     2521 gcagtacgct ggcaaacact tcgtcgtcgt cgatcagtaa aaaattcatg acgcgttctc
     2581 cttgggccag ggcaggatga cggtggcttc taccccgccg cctgcccggt tgcgcaggcg
     2641 cacttcgccg cccaggcgct cgaaggtggc atgcgccagc gccacgccca agcccatgcc
     2701 gcctttgctg ctgggcgcgc ctggcgtgcc agcggcggcg agcagtgacg gcgacagccc
     2761 tgggccgcga ttgcggatgg tcaggcaaaa cgcttcacca gaccagtttg tgtggatgtc
     2821 cacttcttgt ggtgatgcgt cggcagcgtt gttcaagaga ttcagcaagg cggcggtgac
     2881 gccggcatcc agcgccagct ggggcgcggc ggcggggtcg ggctggtagc gcacggcaac
     2941 atccgggcgc agggtgcgcc attggcgcag caactgggcc atgtaggcat ccgccgcctg
     3001 ccggcttgcc gcgcgcgcga cgccggcctc gctggccagc cggttgagcg ctgctttgca
     3061 ggcatccacc tgggtgtgca tgaggtcgag gtcttcgtgc aagtcaggat tgtccagcgg
     3121 cgcgcgccgc cattcgccaa tcagcaccgc cagcgtgctc agtggcgtgc ccagctcgtg
     3181 cgcggcactg gccgccagcg tgcccagcgc cacccattgt tcgtcgcgca attgtttttc
     3241 gcgaaagtcg cgcaaggccc gatcgcgggc gcgcagttcc gcggtcatct tgccaatcag
     3301 cccgaccatc agcgctgctg cgccgcagaa ggtggcccac atgcctaccg tgtgcaaacg
     3361 aaaggccagt gccgggtcgc tgtgtgagcc tggtagaggg atgaaaaaat ccatcagaaa
     3421 agtgtaatca gccactgcca gtaccgccag ccaccaggct tgtcggcgcg gcagcgtcaa
     3481 ggcggcgtac accagcggca gtagatacag cgtcaccagc gggttgttgg cgcctccgct
     3541 gaaaaacagc agcacgccca gcgccgtgca atccagcgcc agcgcgactg ccagcaccca
     3601 gggcgagaca ttgctggcgc ggcgcgtcca cagcgctgcc agcatattgc ccagcgccag
     3661 cgtggccagg gtggccagca tgggaggcca cggcagcgcc atgccatcct gggcggccac
     3721 cagcaggccc aggctggcca gcaggataaa cccccagttg agggcaagca ggcgtgacag
     3781 tgcaacagtg caggattcgg caggcatggc ggatggcgca aaagcgggcg ggcgcttacc
     3841 ttagcacagg atggcgaatg ggcggccgcg gcaaattgtc gcggtcaggc agacgtggtg
     3901 tgcacaatcc acagcgtctt gagtgtcgcc cacaaagcgc tgctggcgtt gcggcgtgac
     3961 tttgccgcgt actgtctgcc gttgtgtgtc tggtcggcac ggctgcgctg cgctttgtta
     4021 gcgtgtgtgc gacatgtttg gccaagcgag cagcgtgcag cgttgtgtgt ggtacagaat
     4081 tatagtttat tgcgatattt taatgtcagc caaaaccaca cgttgagaca gatattatgt
     4141 ttggtggcgt gcacaaaatc tttatggtgt gcttgagtgg cttgacttat ttttctgctg
     4201 ctgtttatgg ctgtgtggct gatgcgtgct ggagtcactt tttgcctgtc gtattaatct
     4261 tttgacctgc ctggatatta tccagaatga tttaaattgc atcaggacaa atggatagat
     4321 agggcggtga caaatgcctt ttgaatgcca tctatcctga ggcaaggcgc ttacgccagc
     4381 tggaacagcg ggtggcccgg ttgcaattgc tggccggtct tgcagatttg ccgccatacc
     4441 ggctggccat gcagtgcgcc gcgcacctct cctgccaggc gcacaaagcc atgggcatcc
     4501 tgttgctcgg ccagtgcccg cagcatgacc accttgattt tgacattgcc gggctcacgg
     4561 ttcagagcgg tttgcagcac gcgcagggca tccttgctgc ggccataggc caggaatacc
     4621 tcggcttcgc cgatggggtc gatgcgccgg gcgcgctgga tgctcaggcg agtttgccgg
     4681 cgtcgccaca tccaatacaa gctgagcgag gcaaataatg ccaatgggat taaagtgttc
     4741 gattcggtaa tcagatcaac catggggcaa actccgtcta acatggggta atcatcgcgt
     4801 gcccgaggaa ttccgacaat tcgtagattt gcctagcgcc cctgtttcaa acacaggttt
     4861 gtgcactcag gccgggaaaa gccgccacgt gtccgggggg aagcacgcca tggtccacgc
     4921 acgtgcggtg tcaacggcgt gggcctttat ccggattatc agaaaaaagc cctatccggt
     4981 gcgggtaatt tggcgaggtg gcggatatgc ggatgcttat catttttttg ataaaataaa
     5041 ttcaatcata tcaaaagctt gaaagcattg ttggtcgtga tgcgatggcc gtgcacatgc
     5101 tggaagtggt tggtttgtca gcgtaaatca gccactgtca gacaaaaaac agccctggcc
     5161 gagggtcacg gcagggatga gcgttaattg gccgatggcc tgcgcatcct gcaaaaatac
     5221 aagatgaacc gatgtttttt tctgattaag ttaatgtcat ggtgtgttta ttccgaagcc
     5281 atgcccccgg agccgactca cggatgtgcg cacggccgcg cagggcggcc gtgacagcag
     5341 atagaggggg aaaaggcacg cgcagtgagc gctcaggcgc ggcggataaa gcgcccttgc
     5401 agcacctcac gcagcaccgc cagcggcacg cccttctcga tcaccgcctg gcccagctga
     5461 cgcagcagca caaagcgcag ctggccttct tccacttttt tgtcgtggcc catatggctg
     5521 atccaggcgt ccacgcccag gtcgggcgac agaatcggca ggccggcgcg ttcgatcaag
     5581 gcggtgacgc gggcgacgtc cgcctcgctg atccagccca gcaggcgcga ggtttccgcc
     5641 gccagcacca tgccggccgc caccgcctcg ccgtgcagcc aggcgccgta gcccatgccg
     5701 gcttcgatgg cgtggccaaa ggtgtggccc aggttgagca gggcgcgcac gccggtttct
     5761 ttttcgtctt cgccaacaat atcggccttc atctggcagc agtggcgcac cgcttcggcc
     5821 agggtggcgc ggtcgcgcgc gcgcagcgcc tcgatgtgtt gctccagcca ggccagaaac
     5881 tgcacatcgc ccagcagcgc gtatttgatc acctccgcca ggccggcgga cagctcgcgc
     5941 tcgggcaggg tgtccagcac cgccatatcg gccagcaccg cgcgcggctg gtagaaggcg
     6001 ccgatcatat tcttgcccag cgggtggttg atggcggttt tgccgcccac cgaggaatcc
     6061 acctgcgcca gcaaggtggt gggaatttga ataaacggcg cgccgcgctg gtagcaggcg
     6121 gcggcaaagc cggtcatgtc gccaatcacg ccgccgccca gggcaatcag cgtggttttg
     6181 cgctcggcgc gcgcgcccag cagggcgtcg aagatcaggt tcagcgtttc ccaggttttg
     6241 taggcttcgc catcgggcag aatcaccggg gtcaccgcca cgccagcggc ctccagcgcg
     6301 gcttgcagcg gcgccaggta gagcggagcg atggtttcgt tggtgatgat ggcggctttt
     6361 ttctgcggca gatgcgccac gatgcgcgcg gcgtcggcgt acagcgattc accaatataa
     6421 atgggatagc gggtgtcggg cagaacaagg tcaagcgtga tcatgcggag aggcgccggg
     6481 caggggagtg gaaggacagg ggctgtggtg cgccagcttt tgcagcaggc ggctgaccag
     6541 caggtgcacg ttctggcgcg aggtgtcgat gatgatgtcg gccacttcgc ggtagagcgg
     6601 gtcgcgctgg gcaaacaggc tttccagctt ggcgcgcggg tcggcggttt gcagcagcgg
     6661 gcggtttttg tcgttttgcg tgcgcagcag caggtcgtcg atgctggcgc gcaggtaaat
     6721 caccgtgccg ttggcggcca gcacccggcg ggtgtccggg tgggtgaccg cgccgccgcc
     6781 agtggccagc acgatgttct ggcgctcggc cagctcggcg atggtcttgc gctcgcgctc
     6841 acgaaaggcc ggttcgccct cgatctcgaa aatcgtggcc acgcgcacac cggcgcgcgc
     6901 ttcgatttcg tggtcggagt cgtagaagga tttgccggtt tcacgcgcca aagtgcgccc
     6961 gacggtggtt ttacccgccc ccatcagccc gaccagaaag agattgccag caacatgtat
     7021 catgcccgcc attctaccca aagacctgcg gcccgcgcga cattgcagcg cagcatcggg
     7081 ccgtgcgtct ggctgggcgg cgccacggtg gggcagggag tcagcctacg accatgtttg
     7141 ctttgcgtaa acggcgtgta acttttacca ttgacggtta cgccgatttt tgcccgagca
     7201 cgtcgatgaa cccgaacctg tcgctgctgc acccctaccc cttccagaaa ctgcgcgagc
     7261 tgtttttggg cgtggtcccc aacccggatc tgaagccggt caatctgtcg attggcgagc
     7321 ccaagcacgc cacgcccacc ctggtgaccg aggcgctgac cgccaatctg ggcgggctgg
     7381 cgcaatatcc ggccacccag ggcagcgagg cgctgcgcca ggcggcggcg cgctggctga
     7441 gccggcgcta tggcctgcgc gggctggaca tccagcgcga aatcctgccg gtgaacggca
     7501 gccgcgaggc gctgtttgcc tttgtgcaag cggtggtgga cgccagcggc gccgagaagc
     7561 cggtggtgat ttcgcccaat cccttctatc agatttacga aggcgcggcg ctattggccg
     7621 gcgccgagcc gtattacctg aactgcctgg ccgaccagcg ctttcagccg gattgggcca
     7681 gcgtgccggc cgaggtgtgg gcgcgcaccc aactggtgtt tgtctgtagc ccgggcaacc
     7741 ccaccggcag cgtgatgtcg ctgaacgatt ggcaggattt gttcgagctg tccgaccgct
     7801 atggctttgt gattgcctcg gacgagtgct attccgaaat ctattttgac gcgccgccgc
     7861 tgggcgggct ggaagccgcc gccacgctgg gccgcgatga ctttgccaat ctggtgatgt
     7921 tcaccagctt gtccaagcgc tccaatgtgc cgggcatgcg ctcgggcttt gtcgccggcg
     7981 acgcccgcgt gctggagaaa ttcctgctct accgcaccta ccatggttcg gcaatgagcc
     8041 cggccattca ggccgccagc gtggcggcct gggacgacga agcccacgtc gagcacaacc
     8101 gccagctgta cgccgaaaaa tttgccgccg tcaccccggt ggtggcgcag gcgctggacg
     8161 tcagcctgcc cgaggccagt ttttatctgt gggcgaaagt gcccggcgac gacgccgaat
     8221 ttgcccgcga gctctaccgc gagcagcacg tcaccgtgct gcccggcagc tatctggcgc
     8281 gcgacgccca cggcgtcaac cccggcgccg gttatatccg cattgccctg gtggctccgc
     8341 tggccgagtg ccaggaggct gccgagcggc tggtgacatt tgcccgcagc cgcgcctgac
     8401 gcccgacggc gctgcgctgc cgggcgccgt catcgttctt tcatttgctc tttgaccttg
     8461 ctctttagaa gatagcccca tgtctgttcc cattcgtatc gccagaggcc ttaacaccat
     8521 gcacccgatc cagaccctga tcgaagaatc ctttgaaaag cgcgccgaca tcaccccggc
     8581 caccgcctcg cccgagctga aatccgccgt gatgcaagtc atcgacgagc tggacaacgg
     8641 ccgcatccgc gtggcggaaa aacacggtga tgactgggtg gtgaaccaat gggtgaaaaa
     8701 agccgtgctg ctgtcgttcc gcatccgcga caacgcgccg atggccggcg gctgcatgca
     8761 gtattacgac aaggtggaca ccaagtttgc cgactacacc gagcgtgaat tccgcgaagg
     8821 cggcttccgc gtggtgccca acgccgtggc ccgtcgcggc agctttatcg gcaagaatgt
     8881 ggtgctgatg ccgtcctacg tgaacatcgg cgcctatgtg gacgaaggca ccatggtgga
     8941 cacctgggcc accgtcggtt cgtgcgcgca aatcggcaag aacgtgcatc tgtccggcgg
     9001 cgtgggcatc ggcggcgtgc tggagccgct gcaagccaac ccgaccatca tcgaagacaa
     9061 ctgctttatc ggcgcgcgct cggaagtggt ggaaggcgtg atcgttggcg aaggctcggt
     9121 gatctcgatg ggcgt
//
LOCUS       DAOPYX010000108         9070 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_9058_length_9070_cov_11.836717, whole genome shotgun sequence.
ACCESSION   DAOPYX010000108 DAOPYX010000000
VERSION     DAOPYX010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 9070)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9070)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9070
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9058_length_9070_cov_11.836717"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(200..1513)
                     /locus_tag="PKN55_07045"
     CDS             complement(200..1513)
                     /locus_tag="PKN55_07045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019921510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNX71034.1"
                     /translation="MTAMDALWMPFTANRQFKANPRMLVSADGMHYTDADGRQVLDGC
                     AGLWCVNAGHNRRPIVEAIQRQAAILDYAPPFQMGHPVEFEAAEALAAMAPKGLSKVF
                     FCNSGSEAADTALKIALAYHRARGDAARIKLIGRERGYHGVNFGGVAVGGMAGNRKHF
                     PASLPCVDHLRHPLDIARNAFSRGEPEFGVELADELEQRIVTLHDPSTIAAVIVEPMQ
                     GSTGVILPPKGYLKRLREICDRHGILLIFDEVITGFGRLGADWAANRFDVLPDILTCA
                     KGLTNGCVPMGAVLVKPAIHDAFMAAAPAHAIEFPHGYTYSGHPLAAAAALATLKLYE
                     DEQLFARAASLQDVFMDAALSLQSASGVKDVRTLGLVAGIELTPWADKPAGARGYAVF
                     QACWERGVMVRFTGDTIAVSPPLIIEPAQIAQLFAVVAEAVEANR"
     gene            1646..3049
                     /locus_tag="PKN55_07050"
     CDS             1646..3049
                     /locus_tag="PKN55_07050"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HNX71035.1"
                     /translation="MTVPFAALQRQQPLAPQIIEQLRERIEQRQLRPGARAPSIRQLA
                     RELQVSPSTVVEAYDRLAALGYLQARRGAGFFVTERRDRPAREHTPKVTELAVDAHWL
                     SRNVYQTDAGALQAGCGWFPPDWFDRDSRQRALKQAARYDSALAEYGHPQGYAPLRRY
                     LEDWLGEAGMAVPAEHILLTQGASQALDLVACCVARPGDVVLVDDPGYCNLLSCLTLR
                     GFTVIGAPWTPHGPDTAALEQLLQTHQPKAFFTNPWLQNPTGASYSPATAYRVLQLAE
                     QYGCYVVEDNVSAELQAVRGQTLAAMAGLARVIYIGSFSKTLSPGLRVGFVAAAPGMI
                     DALTHAKMIAGLTSSSLAERLALAMLTDGRYRKNLERLKTRLAHAQDATLAMFTELGW
                     PLFTAPAGGLFVWASPGDSGPDPLRLAEWARAADILLAPGCLFRPDRADTPWLRFNVA
                     FSHGEALRRFLLGYRRG"
     gene            3246..4436
                     /locus_tag="PKN55_07055"
     CDS             3246..4436
                     /locus_tag="PKN55_07055"
                     /inference="COORDINATES: protein motif:HMM:TIGR00710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HNX71036.1"
                     /translation="MSSRSPLPPAQLIALLGALVAFGPLAIDLYLPALPAIAHGLGAS
                     IEAVQSSVTVFLAGFSLGMLLYGPLSDRYGRRPVLLAGMALFTVASLACWLADSVELL
                     IAARFAQAMGGGAASVLARAVARDVFAPKEAIRQLSRMAMVTSVAPLLAPMLGSAVLA
                     WGGWRSPFAVLCVWGVLSTAVVAWVLAETLPPERRGGLTLGQAFAAYGQMLRQPAAVG
                     LLLAGGLSFAAMFAYITASPDFFIGQQGWSPSAYALVFAANALGIFTANFINSRLAHR
                     LGAATLAGVGSLAGLLAALVMNVALLGAAPAMGWVVGALAVAVSVTGLLGANCVGLLM
                     QRFAQASGAAAALFGASQFGLGMLASAAVSALHDGSGRPMGWVMLACLAGSAAGFVGF
                     WRAR"
     gene            complement(4582..7317)
                     /locus_tag="PKN55_07060"
     CDS             complement(4582..7317)
                     /locus_tag="PKN55_07060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017244827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HNX71037.1"
                     /translation="MSARVQNHITGRLSLRPPQAESLARLKQALDAAPDMLAHDGRDV
                     ASILATLKAEFPTLEDFERDFPSLCFSLATGVGKTRLMGAFIAYLHLAHGINNFFVLA
                     PNLTIYNKLIADFTRNTPKYVFKGIAEFAQQSPLIITGDNYDQTGAVVQDQPMGFAHD
                     VRINIFNISKINSEVRGGKEPRIKRMREVLGDSYFNHLASLDDLVLLMDESHRYRASA
                     GVRSINELKPLFGLEVTATPFVESTRGPVPFKNVVMDYPLARAMEDGFVKEPAAVTQR
                     NFDAKAHSAEELEKIKLEDGIRLHETTKVELLTYARENGVKVVKPFMLVIARDTTHAS
                     QLKTLIESAAFYKGRYADKVIQVDSSRTGAEEEAMIAKLLAVESVDEPTEIVIHVNML
                     KEGWDVTNLYTIVPLRAANARTLIEQSIGRGLRLPYGKRTGVAAVDRLNIVAHDKFQE
                     IIDEANRGDSPIRLKQVILEAPSADDKKVSVQVSSGAMARLGLAEPPSVATSAVSAGA
                     APAAAPAPTFTTERERQAARVVMDVIGKYEVKRELVPTSSALLTPEVQSAILAELTER
                     LKPAQGELLAEVDDAAPTLDLSAVVSKTTEIVVQQTIDIPRIAVVPTGEVTTGFHPFK
                     LGSLPNFQPGQREIVGQELRTNHQFTMSREAGIREQRFEDYIVKKLIDYDDIDYFTQA
                     ELLYDLAGQAVAHYQAQNYSENELHEVFDTYGAELARLIRAEMMAHFWEEASDYEVQV
                     SRGFTELKPCNYTAVAGQTAHNYRETVTELGKIKQMLFDGFSRCLYPLQKFDSDTERR
                     FAVILERDALKWFKPAKGQFQIYYKLGSEQPEYIPDFVAELDSMIFMVETKARVDIDT
                     PEVQAKAVAAARWCKHASDHAASVGGKPWRYLLVPHDDIVESKRLTDFLRFEVKA"
     gene            complement(7314..8390)
                     /locus_tag="PKN55_07065"
     CDS             complement(7314..8390)
                     /locus_tag="PKN55_07065"
                     /inference="COORDINATES: protein motif:HMM:NF018009.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDDEXK nuclease domain-containing protein"
                     /protein_id="HNX71038.1"
                     /translation="MNKNVAVIPADYAAWLAEIKTRVTAARQRAALAANAELIRLYWQ
                     IGRDILERQARQGWGSKVIERLARDLREAFPEMKGFSSSNVKYMRYFAEHCPDLRFGQ
                     QPADQLPWFHLVTLLTKAQPADREWYAGKAIAEGWSRTTLELHIRNRLLQRQGQAITN
                     FDARLPAPHSALAHETLKDPYLFDFLGLGDDAHEREIENALVRHITKFLLELGSGFAF
                     VGRQFRLEVEGDEFFIDLLFYHTRLKCYVVVELKATAFKPEHAGQLNFYLTAVDRQVK
                     APDDKPTIGLLLCKTKKRTVAEYALSGIDKPIGVAEYELVRALPEMLIASLPTVEELE
                     SELEAIDDGESDLPVGQEEKGEQA"
     gene            complement(8390..>9070)
                     /locus_tag="PKN55_07070"
     CDS             complement(8390..>9070)
                     /locus_tag="PKN55_07070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013982133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HNX71039.1"
                     /translation="GTTGAVAHKMGRRWIMVELGEHCHTHIIPRLKKVIDGEDKGGIT
                     ESVNWRGGGGFRYYKLAPSLIVSDRWGNPVINPEYNAAMLAEALAKLEGFTYAPSEVH
                     WWRHGHSSERDFIYVTTQNLSADQLQALSDEVGGDQSLLVCCAAFHGVTAAKASERWP
                     NLTLKKIPKMVLARCEWGHDDYSLNVANLPMAKPEAEAPPTPPSPKGRGRKADVTPDL
                     FGDGGEEA"
BASE COUNT         1522 a         2877 c         2864 g         1807 t
ORIGIN      
        1 tcttttcccc cttttctttg gcgagacaaa gaaaaggggt cgggtgcggg gcgaaacctc
       61 gcccaaaatc cagccctagc aatacccaga gccgctcaca aaacccagcg aagcgatcct
      121 ggctaggcgt gtgaccgcag acagtacaag tcgtacggca aggtcacaca acaacgccag
      181 ggggttttgt gagtggctct tagcgattcg cctccaccgc ctccgccacc accgcaaaca
      241 actgggcaat ctgcgccggc tcgataatca gcggcggcga cacggcaatg gtgtcgccgg
      301 taaagcgcac catcaccccg cgctcccagc aggcctgaaa caccgcatag ccgcgcgcgc
      361 cggccggttt gtccgcccac ggcgtcagct cgatgccggc caccaggccc agcgtgcgca
      421 cgtccttcac ccccgacgcg ctttgcagcg acagggcggc gtccatgaac acgtcctgaa
      481 ggctggccgc gcgggcaaac agctgctcgt cttcatacag cttgagcgtg gccagcgccg
      541 ccgctgccgc cagcgggtgg ccggaatagg tgtagccatg cggaaactcg atggcgtgcg
      601 ccggggcggc ggccatgaag gcgtcgtgga tggccggttt gaccagcacc gcgcccatcg
      661 gcacgcagcc attggtcagg cccttggcgc aggtcaggat gtcgggcagc acgtcaaagc
      721 ggttggcggc ccagtcggcg cccaggcggc caaagccggt gatcacttca tcaaaaatca
      781 gcaaaatgcc gtggcggtcg cagatttcgc gcaggcgctt cagatagccc ttgggcggca
      841 aaatcacccc ggtggagcct tgcatcggct cgacaatcac cgccgcgatg gtggacgggt
      901 cgtgcagggt gacaatgcgc tgctccagct cgtcggccag ctcgacgcca aattccggct
      961 cgccccggct gaaggcgttg cgggcgatgt ccagcggatg gcgcaggtgg tccacgcagg
     1021 gcagcgaggc ggggaaatgc ttgcggttgc cggccatgcc gcccaccgcc acgccgccaa
     1081 agttcacccc gtggtagccg cgctcacggc caatcagctt gatgcgggcg gcgtcgccgc
     1141 gcgcgcggtg gtaggccagg gcgattttca gcgcggtgtc ggccgcttcc gagccggagt
     1201 tgcaaaaaaa caccttggac aaacccttgg gcgccatcgc cgccagcgcc tcggcggctt
     1261 cgaattccac cgggtggccc atctggaatg gcggcgcata gtccaggatg gcggcctgac
     1321 gctggatggc ctcgacaatc gggcggcggt tatggccggc gttcacacac cacaggccgg
     1381 cgcagccatc cagcacctgg cggccgtcgg cgtcggtgta gtgcatgcca tcggcgctga
     1441 ccagcatgcg cgggttggcc ttgaactggc ggttggcggt aaacggcatc cacagggcgt
     1501 ccattgcggt catggcaaag tctccaggtc ggcgggggga tagaaatcgg attgggcaag
     1561 ctcagcgcac atcatggcga caaaaccata cagttggcag tacagttggc cactattgca
     1621 tcgatactgt atgggtgatt tcagcatgac agttccgttt gccgctttgc agcgccagca
     1681 gccgctggcc ccgcaaatca tcgagcagct gcgcgagcgc atcgaacagc gccagctgcg
     1741 ccccggcgcg cgcgcgccgt ccatccgcca actggcgcgc gagctgcaag tcagcccgtc
     1801 caccgtggtg gaggcctacg accggctggc ggcgctgggc tatttgcagg cgcggcgcgg
     1861 cgccggtttt tttgtcaccg agcggcgcga ccgcccggcg cgcgagcaca cgcccaaggt
     1921 caccgagctg gcggtggacg cgcattggct gtcgcgcaat gtctaccaaa ccgacgccgg
     1981 cgccctgcaa gccggctgcg gctggtttcc gcccgactgg tttgaccgcg acagccgcca
     2041 gcgcgcgctc aagcaagccg cccgctacga cagcgcgctg gccgaatacg gccacccgca
     2101 aggctatgcg ccgctgcgcc gctatctgga agattggctg ggagaggccg gcatggctgt
     2161 gccggccgag catatcctgc tcacccaggg cgccagccag gcgctggacc tggtggcctg
     2221 ctgcgtggcg cgtcccggtg acgtggtgct ggtggacgac cccggctatt gcaatctgct
     2281 gtcctgcctg accctgcgcg gctttaccgt gattggcgcg ccgtggacgc cgcacggccc
     2341 ggacaccgcc gcgctggagc agctgttgca aacgcatcag cccaaggcgt ttttcaccaa
     2401 cccctggctg caaaacccca ccggcgccag ctacagcccg gccaccgcct accgcgtgct
     2461 gcaactggcc gagcaatacg gctgctatgt ggtggaagac aatgtctcgg ccgaactgca
     2521 agccgtgcgc gggcaaaccc tggcggccat ggccgggctg gcgcgggtga tctacatcgg
     2581 cagtttctcc aaaaccctgt cgcccggcct gcgcgtgggc tttgtcgccg ccgcgcccgg
     2641 catgatcgac gcgctgaccc acgccaagat gattgccggg ctgacctcat ccagcctggc
     2701 cgaacgcctg gcgctggcca tgctcaccga tggccgctac cgcaaaaacc tggaaagatt
     2761 aaaaacccgg ctggcccacg cccaggacgc cacgctggcc atgttcaccg aactgggctg
     2821 gccgctgttc accgccccgg ccggcggcct gtttgtctgg gccagccccg gcgacagcgg
     2881 ccccgacccg ctgcgcctgg ccgaatgggc gcgcgccgcc gacatcctgc tggcgccagg
     2941 ctgcctgttc cgccccgacc gcgccgacac cccctggctg cgctttaatg tggcgttcag
     3001 ccatggcgag gcgctaaggc ggtttttgct ggggtatcgg cgagggtaaa aatcggtgtc
     3061 accacacagc acgcctgatg gcgcggccag ttggcgatct tgggtcatgt aatagacttg
     3121 cacccgctgg cgagtcgatc cgccaggcag ccctggtgca ccctgtgcac attgccgcca
     3181 gcaagatgac gacgcatcaa gcagccttta ccctgaacgc cccgctctct ttgtcaggaa
     3241 ccctcatgtc ttcccgctcg cccttgccac ccgcgcaact gattgccttg cttggcgcgc
     3301 tggtggcctt tggcccgctg gcgattgacc tgtatctgcc cgcgctgccc gccatcgccc
     3361 acggcctggg cgccagcatc gaagccgtgc aaagttcggt gacggtgttt ctggccgggt
     3421 tttcgctggg catgttgttg tacggccctt tgtccgaccg ctatggccgt cggccggtgt
     3481 tgttggccgg catggccttg ttcactgtcg ccagcctggc gtgctggctg gccgacagcg
     3541 ttgagttgtt gattgccgcc cgctttgccc aggccatggg cggcggcgcg gcttcggtgt
     3601 tggcgcgggc ggtggcgcgc gatgtgtttg cgcccaaaga ggcgattcgt cagttgtcgc
     3661 gcatggcgat ggtgacgtca gtggcaccgc tgctggcgcc gatgctgggc agcgcggtgc
     3721 tggcctgggg cggctggcgc agcccgtttg ccgtgctgtg tgtctggggg gtgctcagca
     3781 ccgccgtggt ggcctgggtg ttggcggaaa ccttgccgcc cgagcggcgc ggcggcttga
     3841 cgctgggcca ggcgtttgcc gcctacggcc agatgctgcg ccagccggca gccgtgggct
     3901 tgttgctggc cggtggtttg tcgtttgccg cgatgtttgc ctatatcacc gccagcccgg
     3961 acttttttat tggccagcaa ggctggtcgc ccagcgctta tgcgctggtg ttcgccgcca
     4021 atgcgctggg gatttttacc gccaatttca tcaacagccg cctggcgcac cggctgggcg
     4081 cggccacgct ggccggggtg ggcagcctgg ccggtttgtt ggcggcgctg gtgatgaatg
     4141 tggcgctgct gggcgcggcg ccggcgatgg gctgggtggt gggcgcgctg gcggtggcgg
     4201 tgagcgtgac cggcttattg ggcgccaatt gcgtggggct gttgatgcag cgctttgccc
     4261 aggccagcgg cgcggcggcg gctttgtttg gcgccagcca gtttggcttg ggcatgctgg
     4321 ccagcgcggc ggtgagcgcg ctgcacgacg gcagcggccg gccgatgggc tgggtgatgc
     4381 tggcgtgcct ggccggctcg gcggcggggt ttgtcgggtt ttggcgcgcg cggtgatgcg
     4441 ggggcaatat gctcaagccg ccaccaagca tcacgtcgat tttcattcgg cgatgatttt
     4501 ggtggcttta agatgcctgt cgcgaacaca gtgggcgcgt gctatctcct tacctgaggc
     4561 tgattgcggc gctgcgacct ctcaagcttt cacctcaaac cgcaagaagt cagttaggcg
     4621 tttcgactcc acaatgtcat cgtgcggcac aagcaggtag cgccaaggct tgccgcctac
     4681 tgaggcagca tgatctgacg catgcttgca ccagcgcgca gcagctaccg ctttggcctg
     4741 tacttccggc gtgtcgatat caacccgagc tttggtttcc accatgaaga tcatagaatc
     4801 aagttcggcc acgaagtcgg ggatgtattc cggctgttcg ctgcccagct tgtagtaaat
     4861 ctgaaactgc cccttggcag gcttgaacca cttcagcgca tcacgttcca gaataaccgc
     4921 aaagcgtcgc tcggtatccg aatcaaactt ttgcagtggg tacaggcagc gcgagaagcc
     4981 gtcaaacagc atttgcttga tcttgcccag ttcggtcacc gtttcacggt agttgtgtgc
     5041 cgtctgcccg gcgaccgccg tgtagttgca cggtttgagt tctgtgaaac ctcggctgac
     5101 ctgcacctcg tagtcggagg cttcttccca gaagtgggcc atcatttcgg cccggatcag
     5161 gcgggccagc tcggcgccat aggtgtcgaa cacttcatgc agctcatttt ccgagtagtt
     5221 ctgcgcctgg tagtgagcta ccgcctgacc ggccaagtcg tagagcaact cggcctgcgt
     5281 gaagtagtcg atgtcgtcgt agtcgatcag cttcttgacg atgtagtcct cgaagcgctg
     5341 ctccctgatg cctgcttcac ggctcatggt gaactgatgg ttcgtgcgca gctcttggcc
     5401 gacgatctcc cgttgccccg gctggaagtt gggcagtgag cccagcttga acgggtggaa
     5461 gccagtggtg acctccccgg tgggcaccac cgcgattcgc ggaatgtcga tggtttgctg
     5521 caccacgatt tccgtggtct tggacacgac ggccgacaaa tcaagcgtcg gtgcggcatc
     5581 gtccacttca gccagcaact ccccctgggc aggcttcagc cgctcggtca attcagccag
     5641 aatggccgat tgcacctccg gggtgagcag cgcactgctc gtcggcacga gttcacgctt
     5701 cacctcgtac ttgccgatca catccatcac cacgcgagcg gcttggcgct cgcgctccgt
     5761 ggtgaacgtg ggcgctggcg cagcggccgg cgccgccccc gcgctgacag cacttgtcgc
     5821 aacggacggg ggctccgcaa gacccagccg ggccatggcg cctgagctga cctgcacgct
     5881 caccttcttg tcgtcagcgc ttggcgcctc taggatcacc tgcttcagcc ggatcggcga
     5941 gtcacctcgg ttcgcctcat caatgatctc ctggaacttg tcgtgggcca cgatgttcag
     6001 ccgatccacc gctgccacgc ccgtgcgctt gccgtaaggc agacgcaaac cgcgaccgat
     6061 ggactgctcg atcagcgtgc gcgcattggc ggcccgcagg ggcacgatgg tgtagaggtt
     6121 cgtcacgtcc cagccttctt tgagcatatt gacgtggatg acaatttcgg tgggttcatc
     6181 cacgctttcc acggccaaaa gtttggcgat catcgcctct tcttctgccc ctgtccggct
     6241 ggaatccact tggataacct tgtccgcata acgccctttg taaaaggctg ccgactcaat
     6301 cagcgttttc agttgtgagg cgtgcgtggt atcccgtgcg atcacgagca tgaagggctt
     6361 gaccaccttc acgccgttct ctcgggcata agtcagcaac tccaccttgg tggtttcgtg
     6421 caggcgaatg ccatcttcca gcttgatctt ttcgagctct tccgccgagt gcgccttggc
     6481 atcgaaattg cgctgagtca cggccgcagg ctctttgacg aagccgtctt ccatcgcccg
     6541 cgccagtggg tagtccatca ccacgttctt gaagggcaca gggccgcgcg tggattccac
     6601 gaacggggtg gccgtcacct caagtccaaa taggggcttc agttcgttga tggaccgcac
     6661 gccggcactg gcccggtagc ggtgcgactc gtccatcaac agcaccaagt cgtccaggct
     6721 ggccaggtgg ttgaagtagc tgtctcccag cacctcccgc atgcgcttga tgcgtggctc
     6781 tttgcctccg cgaacctcgg aattgatctt ggaaatattg aagatgttga tgcgcacatc
     6841 gtgggcaaag cccataggct gatcctgaac caccgcaccg gtctggtcgt agttgtcgcc
     6901 ggtgatgatc agcggggact gctgagcgaa ctcggcgatt cccttgaaca cgtacttggg
     6961 cgtgttgcgc gtgaagtcgg caatcagttt gttgtagatc gtcaggttgg gcgccagcac
     7021 aaagaagttg ttgatgccgt gcgccagatg caggtaggcg atgaacgcac ccatcagccg
     7081 cgtcttgccc acgccggtcg ccagcgagaa gcacagcgag gggaagtcgc gttcaaagtc
     7141 ctccagcgtc gggaactctg ccttcagcgt ggcaagaatg gacgcaacat cacgcccatc
     7201 gtgagccagc atgtccggcg cagcatccag ggcctgtttc agccgggcca gcgattcggc
     7261 ttgtggcggg cgtaaggaca aacgcccggt gatatggttt tgcacgcggg cgctcatgct
     7321 tgttcccctt tttcttcttg gccaacgggt aagtcggact caccgtcatc aatcgcctcc
     7381 agctcgcttt ccaactcctc caccgtcggc aaactggcga tcagcatttc aggtagcgca
     7441 cgcacgagtt cgtattcggc cacgccaatc ggcttgtcta tgcctgacag tgcatattcc
     7501 gcaaccgtgc gcttcttcgt cttgcacagc agcaggccga tggtgggctt gtcgtcgggt
     7561 gcttttacct gcctgtccac cgcagtcagg taaaaattta attgcccggc atgttcgggc
     7621 ttgaaggccg tggctttcag ctctaccacc acatagcact tcaggcgcgt atggtaaaaa
     7681 agcaagtcga tgaaaaactc atcgccctcg acctccagcc gaaattgacg ccccacaaag
     7741 gcaaagccgc tacccaactc cagcaggaac ttggtgatgt gccgcaccaa ggcgttctcg
     7801 atctcgcgct cgtgggcgtc atcgcccaga ccgagaaagt cgaacagata ggggtctttc
     7861 aaggtctcgt gcgccagtgc cgaatgcggt gcgggcagac gggcatcaaa gttggtgatg
     7921 gcctgtcctt ggcgttgcag caagcggttg cggatgtgaa gctccagcgt cgtgcgcgac
     7981 caaccttccg cgatggcctt gccggcatac cattcgcggt cggcgggttg cgccttggtc
     8041 agcagggtga ccaggtgaaa ccagggcaat tggtcagcag gctgctgacc aaatcggagg
     8101 tcggggcagt gctcggcgaa atagcgcatg tatttcacgt tgctgctgga aaaccccttc
     8161 atctccggaa aggcttcgcg caggtcacgc gccagccgct cgatgacctt gctgccccag
     8221 ccctgtcggg cctgccgctc cagaatgtcc cggccaatct gccagtagag gcgaatcaat
     8281 tccgcattcg cagccagcgc cgcacgctga cgggctgccg tcacgcgggt cttgatctcg
     8341 gccagccagg cggcgtaatc ggcgggaatc accgcaacat ttttgttcat cacgcctcct
     8401 ccccgccgtc cccaaacagg tcaggcgtga cgtctgcttt tctccctcgc cctttgggag
     8461 agggcggggt tgggggcgct tctgcttctg gtttcgccat cggcaggttc gccacattca
     8521 ggctgtagtc gtcatgaccc cattcgcagc gggccagcac catcttcgga atcttcttca
     8581 gtgtcaggtt cggccagcgt tcggatgctt tggcggcggt gaccccgtgg aaggccgcgc
     8641 agcagaccag caggctttga tcgccgccca cttcgtcgga cagcgcctga agttgatctg
     8701 ccgacaggtt ctgcgtggtg acgtagatga agtcccgctc gctggagtgc ccgtgccgcc
     8761 accaatgcac ctctgatggg gcgtaggtga agccttctaa cttagcaagg gcttcggcca
     8821 gcatagcggc gttgtattcg gggttgataa ccggattgcc ccaacggtcg ctgacgatca
     8881 ggctaggggc gagcttgtag tagcggaagc caccgccgcc tcgccagttc acgctttcgg
     8941 tgatgccacc cttgtcttct ccgtcaatca cctttttcag gcgcgggatg atgtgagtgt
     9001 ggcaatgctc gcctaattcg accatgatcc agcgacggcc cattttgtgg gcaactgcgc
     9061 cggtggtgcc
//
LOCUS       DAOPYX010000109         8999 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_9177_length_8999_cov_10.944432, whole genome shotgun sequence.
ACCESSION   DAOPYX010000109 DAOPYX010000000
VERSION     DAOPYX010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 8999)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8999)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8999
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9177_length_8999_cov_10.944432"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..920
                     /locus_tag="PKN55_07075"
     CDS             <1..920
                     /locus_tag="PKN55_07075"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNX71040.1"
                     /translation="AMCADFTACPDPLARAPRAGGRASVLIAPLSLQGALSAVVWRDT
                     AGLALNAAQKLNHFPATPLVCLSWFQGMDIGLIESSEGGAPRWRPLDAALTWSGSQSH
                     PLLSWSPSVGRAGMACFPVDVAHALFGVDPHDLLDRFAPAQAVLDASWQGLLDDLLAS
                     ANPHAALAALEHHLAPRWQALRQRADPAPSLAQIGRHWVQRLAWQAMQWRDSHGERQI
                     ERRIKRYSGRSLREWRMLVKTEGALFAGLQRHHAGLPLDLAALAHEQGFADQAHLSRA
                     TKRITGFAPAEFARRFVEDESFWVYRLWV"
     gene            complement(928..1200)
                     /locus_tag="PKN55_07080"
     CDS             complement(928..1200)
                     /locus_tag="PKN55_07080"
                     /inference="COORDINATES: protein motif:HMM:TIGR02385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HNX71041.1"
                     /translation="MSSGWKVEYTETALAALRKLDKPVARRIMDYLDAHIVGQDAPRT
                     TGKRLQGPLAGLWRYRVGDMRVICQIEDQVLRVLVLRLGKRSEIYR"
     gene            complement(1178..1405)
                     /locus_tag="PKN55_07085"
     CDS             complement(1178..1405)
                     /locus_tag="PKN55_07085"
                     /inference="COORDINATES: protein motif:HMM:NF017333.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraY domain-containing protein"
                     /protein_id="HNX71042.1"
                     /translation="MSTVVELTPDIEQRLEALAARTGRSKREQLQDIIAQGVEDMEDY
                     YLSAEVLKRVQQGQERVYSAEAVRRELGLEG"
     gene            complement(1497..2876)
                     /locus_tag="PKN55_07090"
     CDS             complement(1497..2876)
                     /locus_tag="PKN55_07090"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014086565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HNX71043.1"
                     /translation="MAISVFDLFKIGIGPSSSHTVGPMRAARQFVSRLARDGQLAAVA
                     QVRVELFGSLGATGKGHGSDTAVMLGLHGESPDLIDPDHAPAILNAIRASGQLSLLGQ
                     HPIAFIEREHLILHKRKSLPYHPNAMRCEASDASGALLDARTYYSVGGGFVVDDSALS
                     SGFVPPGPQQLRHPFASARELLALCREHQQPISQIMLANELAWRDEASVRAGLLNLWA
                     VMQACVARGCQREGVLPGGMHVRRRAAELYRTLCATPEAALRDPLTVMDWVNLYALAV
                     NEENAAGGRVVTAPTNGAAGIIPAVLHYYTRFVPGANADGVVRFLLTAGAIGILFKLN
                     ASISGAEVGCQGEVGSACSMAAGALAEVLGGTPEQVENAAEIGMEHNLGLTCDPIGGL
                     VQVPCIERNAMASIKAINAARMALRGDGQHHVSLDRVIRTMRDTGRDMSSKYKETARG
                     GLAVNWIEC"
     gene            3510..4796
                     /locus_tag="PKN55_07095"
     CDS             3510..4796
                     /locus_tag="PKN55_07095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008954993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HNX71044.1"
                     /translation="MKMNRLTTWIGCALVLGVIVGYACNTLAASPAQAKEIASYFSIL
                     TDIFLRLIKMIIAPLVFATLVAGLAGMGDARSVGRIGSRALGWFIGASFCSLFIGLIF
                     ANVLHPGHALNVPLPESAAGLNLKTGALNLKDFITHVFPKSIIEAMAGNEVLQILVFA
                     LFFGLALGHLHSQTARSLVNTMDEVVHVMLKVTDYVMRFAPFGVFGAVAGAITTNGLG
                     MLVIFGKFMLSFYVALAVLWLVLIGAGYLVLGRDVFRLLKLVRGPLLVGFSTASSESV
                     YPKLMEQLEKFGIKTRVTGFVLPLGYSFNLDGSMMYTTFLALFIAQAYDIPMTLTAQI
                     TMLLVLMVSSKGIAGVPRASLVVVAAVLPMFGLPEAGLLLILGIDHFLDMGRTVTNVL
                     GNAIATAVVAKWEGAIDPVDPALAEREESLPVEGEA"
     gene            5110..6192
                     /gene="dinB"
                     /locus_tag="PKN55_07100"
     CDS             5110..6192
                     /gene="dinB"
                     /locus_tag="PKN55_07100"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012697303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HNX71045.1"
                     /translation="MNPPARKIIHIDCDCFFAAVEMRDHPEWRDVPLAVGGAPAARGV
                     IATCNYPARAFGIHSAMPSAQALKRCPHLVLVPGDMARYREASQAVRAVFERFTSLIE
                     PVSLDEAYLDVSGQPHCQGSATRMAEAIRAAIRHEVGITASAGIAPNKMLAKIASDWN
                     KPDGQWLIRPQDIAEFMPSLPVHKLNGVGPATAARLAALGVSTCGQLQQLPLAELAQR
                     FGRFGERLHQMARGEDTRPVRSERVRKSLSVERTYPVDLPDLAACAAQLPSLLAALTA
                     RLAPWLASGGQFRGAWVKVRFADFHHTTVEDIHAPLTEPALHALLVQALARGGQPVRL
                     LGVGVRLMPPAAPHSARHSQQLELFA"
     gene            complement(6250..7551)
                     /locus_tag="PKN55_07105"
     CDS             complement(6250..7551)
                     /locus_tag="PKN55_07105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012698644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3422 domain-containing protein"
                     /protein_id="HNX71046.1"
                     /translation="MTDTFFLHPQRQLLNNEAHVRTPVSVPSPAQVTSLAFFFDDDGS
                     RQRQALSRLAAQLGLPLPAADAVQYQAELPGLRLNWGLHTEFARYTLLRRGRTGEPFA
                     DSAFEGLPLDWLSNQADPLLVAIHAVILPQEDAGAADTETLAQQWFAGNDLVGASIGD
                     GNAMALTDLRLHPDRNLGAGVTRLLVLDYGMSASQRGRMLQRLFELETYRMLALLALP
                     VAKAQVRVMDDLGRRMRALTGQMSGKSGARSSDDALLDQLTELAADLEDAISSTQYRF
                     SAGQAYYRQVERRIEELREARLGGVQPFREFTIRRLAPAMATCETIGNRQTQLAARIQ
                     RATALLRTRVEVSLQGQNQALLASMDRRAELQLRLQETVEGLSVGVLTYYAVGLIGYL
                     AKALKVAGAPVNPELVTGLAIPLVAAGVWWGTQKMKKSLGH"
     gene            7785..8774
                     /locus_tag="PKN55_07110"
     CDS             7785..8774
                     /locus_tag="PKN55_07110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018746437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HNX71047.1"
                     /translation="MKTKLSLLLVGALALAPSAWAASLLNVSYDPTRELYKEINPAFI
                     KSWQSQTGETLTIQQSHGGSGKQARSVIDGLAADVVTLALAHDIDEIAEKTKLLPSNW
                     QTRLPHNSSPYTSTIVFLVRKGNPKAIKDWGDLVKPGVQVITPNPKTSGGARWNYLAA
                     WGYALKQPGGNEAKARDFVKALFKNVPVLDTGARGSTMTFVERGIGDVLLAWENEALL
                     AKKELGPEKFDIIAPPLSILAEPPVAVVDKNAEKKGTLKQANAYLKFLYTPEAQTIIA
                     ENYFRPIDPTVASKYAAQYPKLKLFTIQDLGGWRKAQTTHFSDGGVFDQINAR"
BASE COUNT         1501 a         2916 c         2901 g         1681 t
ORIGIN      
        1 ccgccatgtg cgccgatttc accgcctgcc ccgacccgct cgcccgcgcc ccgcgcgctg
       61 gtgggcgcgc cagcgtgctg attgccccgc tcagcctgca aggcgcgctc agcgcggtgg
      121 tgtggcgaga cacggctggg ctggcgctga acgccgcgca aaagctcaat cattttcctg
      181 ccacgccgct ggtgtgtctg tcatggtttc agggcatgga catcggcttg atcgaatcca
      241 gcgagggcgg cgcaccgcgc tggcggccgc tggatgctgc gctgacctgg tcgggcagcc
      301 agtcgcaccc actgctcagt tggtcgccca gtgtcgggcg cgccggcatg gcgtgttttc
      361 cggtggatgt cgcccacgcg ctgtttggcg tggacccgca cgacctgctg gaccgctttg
      421 ccccggcgca ggcggtgctg gacgcctcgt ggcaaggctt gctggacgat ttgctggcca
      481 gcgccaaccc gcacgccgcg ctggcggcgc tggagcacca cctggccccg cgctggcagg
      541 cgctgcgcca acgcgccgac ccggcgccat cgctggcgca aatcggccgc cactgggtgc
      601 agcggctggc ctggcaggcg atgcaatggc gcgacagcca cggcgagcgc cagatcgaac
      661 ggcgcatcaa gcgctacagc ggccgctcgc tgcgcgaatg gcgcatgctg gtgaaaaccg
      721 aaggcgcgct gtttgccggc ctgcaacgcc accacgccgg cctgccgctg gacctggccg
      781 cgctggcgca cgagcaaggc tttgccgacc aagcccacct gagccgcgcc accaaacgca
      841 tcaccggctt tgccccggcg gagtttgccc ggcgctttgt tgaggatgag tcgttttggg
      901 tgtatcggtt gtgggtgtga gtgaggctca gcggtagatt tcgctgcgct tgcccaggcg
      961 cagcaccagc acgcgcaata cctggtcttc gatctgacaa atcacccgca tgtcgcctac
     1021 ccgatagcgc catagcccag ccagcggccc ttgcaaacgc ttgcctgtgg tgcgcggcgc
     1081 atcctggccg acaatatgcg catccagata gtccatgatc cgtctggcca ctggcttgtc
     1141 gagcttgcgc aaggcggcta acgcggtttc ggtgtattca accttccaac ccgagctcac
     1201 gccgcactgc ctctgcgctg tacacgcgct cttgcccttg ctgaacgcgc ttgagcactt
     1261 cggcggacag ataatagtcc tccatgtcct ccacgccctg ggcgatgatg tcctgtaact
     1321 gctcgcgttt gctgcgcccg gtgcgcgccg ccagtgcttc gagacgttgc tcgatgtcgg
     1381 gggtaagctc cactacggtg ctcatgtgtg cgttctcctg cggaattgtg ccgcgccatc
     1441 aagcacatgg agcggtttct ggcagtgtat cgcagtgggg agcgaaggca atatcctcaa
     1501 cactcaatcc aattcaccgc cagccccccg cgcgcggttt ctttgtattt gctgctcatg
     1561 tcccgacccg tatcgcgcat ggtgcgaatc acccggtcca gcgagacatg gtgctggccg
     1621 tcaccgcgca gggccatgcg ggcggcgttg atcgccttga tcgaggccat ggcgttgcgt
     1681 tcgatgcagg gcacttgcac caggccgcca atcgggtcgc aggtcaggcc cagattgtgt
     1741 tccatgccga tttcggcggc gttttccact tgttccggcg tgccgcccag cacttcggcc
     1801 agcgcgccgg cagccatcga gcaggccgag cccacttcgc cctggcagcc cacttcggcg
     1861 ccggagatcg aggcgttgag tttgaacagg atgccaatcg ccccggcggt gagcagaaag
     1921 cgcaccacgc cgtcggcgtt ggcgccgggc acaaagcggg tgtagtagtg cagcacggcg
     1981 gggatgatgc cggcggcgcc gttggtgggg gcggtgacca cgcggccgcc ggcggcgttt
     2041 tcttcgttga ctgccagcgc atacaaattc acccagtcca tcaccgtcag cgggtcgcgc
     2101 agcgcggctt cgggcgtggc gcacagcgtg cggtagagct cggcggcgcg gcggcgcacg
     2161 tgcatgccgc cgggcagcac gccttcgcgc tggcagccgc gcgccacaca ggcttgcatc
     2221 accgcccata gattcagcag gccggcgcgc acgctggctt cgtcgcgcca ggccagttcg
     2281 ttggccagca tgatctggct gatcggttgc tgatgctcgc ggcacagcgc cagcagttcg
     2341 cgcgcgctgg caaacgggtg gcgcagttgc tgcgggccgg gtgggacaaa gccgctgctc
     2401 agcgcgctgt cgtccaccac aaaaccgccg cccaccgagt aataggtgcg cgcatccagc
     2461 agcgcgccgc tggcgtcgct ggcttcgcag cgcatggcgt tggggtggta gggcaggctt
     2521 ttgcgtttgt gcagaatcag atgctcgcgc tcgataaagg caatcgggtg ctggcccagc
     2581 aggctgagct ggccgctggc gcggatggcg ttcaggatgg ccggggcgtg gtcgggatcg
     2641 atcaggtcgg ggctttcgcc atgcaggccc agcatcaccg ccgtgtcgct gccgtggccc
     2701 ttgccggtgg cgcccaacga gccaaacagc tcgacgcgca cttgcgccac agcggcaagc
     2761 tggccatcgc gcgccagccg gctgacaaac tgacgggcgg cgcgcatcgg gccgacggtg
     2821 tgcgagctgg aagggccgat gccgattttg aacaggtcga atacgctgat ggccatgctg
     2881 agtcctcgtg ggtgatgcgc gccttgggtg agctcacaaa acacttctgg tgttgttgcg
     2941 caaccttgcc aggcttctcg ccctgtctgt acgggctagg cgtgtgaccg cagacagtac
     3001 aagaagtacg acaaggtcac acaacaacgc caggagggtt ttgtgagcag cgcttattgg
     3061 gcacgccgcg tgccggtggt gcagtgtagc gcggcggtgg cgcgcggcgc gtgactgggc
     3121 gcgacatcga atgcgggcag atgcgacatg tttgggcggc tcgcggccgg gcggcggtgg
     3181 gcaaaaccgt ctgcgccagc gcgtggcgca gtgaatttta tgcaatggcc ggtatggtct
     3241 gttcggtttt ccgactattt cagtcggact tggttcggtt ttccgtttca aatatgcttt
     3301 atgaatttga tgttttattg taaatcatga gcttagcctg tgtgttgcgc tggcacgaag
     3361 cgtgcaatag tcggcgcccg cgtcacacgg ccgcttcatg ttgatggcat tttctgtctg
     3421 tcatgaattg gcaatttttg ccccttgtga cgccattcgc cgcttgcgaa cgcgcgcggt
     3481 ggatgtccat gacacgaaag gaaaccatca tgaaaatgaa ccggctgacc acctggatcg
     3541 gctgcgccct ggtgctgggg gtgattgtgg gttatgcctg caacaccctg gccgcctcgc
     3601 cggcgcaggc caaggaaatc gcctcgtatt tcagcatcct caccgatatt tttctgcgcc
     3661 tgatcaagat gatcatcgcc ccgctggtgt tcgccaccct ggtggctggc ctggcgggca
     3721 tgggtgacgc ccgttcggtg ggccgcatcg gcagccgggc gctgggctgg ttcatcggcg
     3781 cctcgttttg ctcgctgttc atcggcctga tttttgccaa tgtgctgcac cctggccacg
     3841 cgctgaacgt gccgctgcca gaatccgccg ccgggctgaa cctgaaaacc ggcgcgctga
     3901 acctgaaaga ctttatcacc catgtcttcc ccaagagcat catcgaggcc atggccggca
     3961 atgaggtttt gcagattctg gtgtttgcgc tgttctttgg cctggcgctg ggccatctgc
     4021 acagccagac cgcccgctcg ctggtgaaca ccatggatga agtggtgcac gtgatgctga
     4081 aagtcaccga ctatgtgatg cgctttgccc cgtttggcgt gtttggcgcg gtggccggcg
     4141 ccatcaccac caatggcctg ggcatgctgg tgatttttgg caaattcatg ctgagtttct
     4201 acgtggcgct ggcggtgctg tggctggtgc tgattggcgc cggttatctg gtgctgggcc
     4261 gcgatgtgtt ccgtttgctc aagctggtgc gcgggccgct gctggtgggg ttttccaccg
     4321 cctccagcga gtcggtgtac cccaagctga tggagcagct ggaaaaattc ggcatcaaaa
     4381 cccgggtgac gggctttgtg ctgccgctgg gttattcgtt taacctggat ggctcgatga
     4441 tgtacaccac cttcctggcg ctgttcatcg cccaggctta cgacatcccg atgaccctca
     4501 ccgcccagat caccatgctg ctggtgctga tggtgtcgtc caagggcatt gccggcgtgc
     4561 cgcgcgcctc gctggtggtg gtggccgccg tgctgccgat gtttggcctg cccgaggccg
     4621 gcctgttgct gattctgggc attgaccact tcctggacat gggccgcacc gtcaccaatg
     4681 tgctgggcaa cgccattgcc accgccgtgg tggccaagtg ggaaggcgcc atcgacccgg
     4741 tggacccggc gctggccgag cgtgaagagt cgctgccggt ggaaggcgaa gcctaagcgc
     4801 cgcgcacaaa accttcctgg cgttgttgtg tgaccttgcc gtacttgttg tactgtctgc
     4861 ggtcacacgc ctagccagga ccgcttcgct ggattttgtg aacgactcta aacaccacct
     4921 caggccggcg cgtgcatgac agcacccgct gtcagcgcgg gccggctttg gcatactggc
     4981 ggcgctgacc aaaccctcct ggcgttgttg cgcggccttg ccggacgact ttacggtctg
     5041 tggccgcagc ctggccagga tcgctgcgcg gggttgtgtg agcgacgctt attgcccgcc
     5101 cggatgccga tgaacccgcc tgcccgcaaa atcattcaca tcgattgcga ctgctttttt
     5161 gccgccgtgg aaatgcgcga ccaccccgaa tggcgcgatg tgccgctggc ggtgggcggc
     5221 gcgcctgccg cgcgtggggt gattgccacc tgcaattatc cggcgcgcgc ctttggcatt
     5281 cactccgcca tgccctcggc acaggcgctc aagcgctgcc cgcacttggt gctggtgccc
     5341 ggcgacatgg cgcgctaccg cgaagcttca caggcggtgc gcgcggtgtt cgagcgcttt
     5401 accagcttga tcgagccggt gtcgctggac gaagcctatc tggatgtcag cggccaaccg
     5461 cattgccagg gcagcgccac gcgcatggcc gaggcgattc gcgccgccat ccgccacgag
     5521 gtgggcatca ccgcctcggc cggcattgcc cccaataaaa tgctggccaa gatcgccagc
     5581 gactggaaca agcccgacgg ccaatggctg atccgcccgc aggacatcgc cgaattcatg
     5641 cccagcttgc cggtgcataa gctcaacggc gtcggcccgg ccaccgctgc acggctggcg
     5701 gcgctggggg tgagcacctg cggccagttg cagcagctgc cgttggccga gctggcgcag
     5761 cgctttggtc gctttggcga gcgtctgcac cagatggcgc gcggcgagga tactcggccg
     5821 gtgcgcagcg agcgggtgcg caaatcgctc agcgtcgagc gcacctatcc ggtggacttg
     5881 cccgacctgg ccgcctgcgc cgcgcagctg cccagcctgc tggccgcgct gaccgcgcgg
     5941 ctggcgccgt ggttggccag cggcggccag tttcgcggcg cctgggtgaa agtgcgcttt
     6001 gccgattttc atcacaccac ggtggaagac attcatgccc cgcttaccga gccggcgctg
     6061 catgcgctgt tggtgcaggc attggcgcgc ggcggccagc cggtgcgcct gctgggggtg
     6121 ggcgtgcggc tgatgccgcc ggccgcgcca cacagcgcgc gacacagcca gcaattggag
     6181 ctgtttgcct gagcaggacg ccaacgccgc acaacgtttt atgggcgaca cgcactcggt
     6241 gaataacgct cagtgaccca ggcttttttt cattttttgc gtgccccacc acaccccggc
     6301 ggccaccagc gggatggcca ggccggtgac caattcgggg ttgaccggcg caccggccac
     6361 tttcagcgcc ttggccaggt agccaatcaa gcccaccgcg tagtaggtca gcacgcccac
     6421 cgacagccct tccacggttt cttgcagacg cagttgcagc tcggcgcggc ggtccatcga
     6481 cgccagcagc gcctgattct gcccttgcag cgacacctcc acccgcgtgc gcagcagcgc
     6541 ggtggcgcgc tggatgcgcg cggccagctg ggtttgccgg ttgccgatgg tttcgcaggt
     6601 ggccatggcc ggcgccagcc ggcggatggt gaactcccgg aagggctgca ccccgcccag
     6661 ccgcgcctcg cgcaattcct caatgcgccg ctccacctgg cggtaatacg cctgcccggc
     6721 cgagaagcga tattgcgtgc tggaaatcgc gtcctccagg tcggcggcca gttcggtgag
     6781 ctggtccagc aaggcgtcgt cgctgctgcg cgcgccgctc ttgccgctca tctgcccggt
     6841 gagcgcgcgc atgcgccggc ccagatcgtc catcacccgc acctgcgcct tggccaccgg
     6901 cagcgccagc aaggccagca tgcgataggt ttccagctcg aacagccgtt gcagcatgcg
     6961 cccgcgctgc gaggcgctca tgccgtaatc cagcaccaac aggcgggtga cgccagcgcc
     7021 cagattgcgg tccgggtgca ggcgcaggtc ggtcagcgcc atggcgttgc cgtcgccaat
     7081 gctggcgccc accagatcgt tgccggcaaa ccactgctgc gccagggttt ccgtatcggc
     7141 ggcgccggcg tcttcctgcg gcaggatcac cgcatgaatc gccactagca gcgggtcggc
     7201 ttggttggac agccaatcca gcggcaagcc ttcaaacgcg ctgtcggcaa acggctcgcc
     7261 ggtgcggcca cggcgcagca gggtgtagcg ggcaaactcg gtgtgcagcc cccagttcag
     7321 ccgcaggccg ggcagctcgg cctggtattg caccgcgtcg gccgccggca agggcaggcc
     7381 cagctgcgcc gccagccgcg acagcgcctg acgctggcgg ctgccgtcgt cgtcaaagaa
     7441 aaacgccagc gaagtcacct gcgcaggcga aggcacggac accggcgtgc gcacatgggc
     7501 ttcgttgttc agcaactggc gctgcggatg cagaaaaaaa gtatcggtca tggtgtggtg
     7561 tgacaaggca agagtaatcg gcgcgaaaat aaaacaaatg tttgaatctg ggcaatcctg
     7621 tcatgccggg cgtccgtggc ggcccggcag gctgtggata ggggtttacc accttttaga
     7681 ctattttgct attagcaatt ggtttgaagt tttttctggg aatatcaaat tcccggatac
     7741 tggccctcaa catctgcaca cccactaatc tgtcaggagc catcatgaaa accaaattgt
     7801 cactgctgct ggtcggcgcg ctggcgctgg ccccgtcggc ctgggccgcc tcgctgctga
     7861 atgtgtccta cgaccccacc cgcgaactgt acaaggaaat caacccggcc tttatcaaat
     7921 cctggcagag ccagaccggc gaaacgctga ccatccaaca atcgcacggc ggctcgggca
     7981 agcaggcccg ctcggtgatt gatggcctgg cggcggatgt ggtcaccctg gcgctggcgc
     8041 acgacatcga cgaaatcgcc gaaaaaacca aactcctgcc tagcaattgg cagacccgcc
     8101 tgccgcacaa cagctcgccc tacacctcga ccattgtttt tctggtgcgc aagggcaacc
     8161 ccaaggccat taaagactgg ggcgacctgg tgaaacccgg cgtgcaagtg atcaccccca
     8221 acccgaaaac ctcgggcggc gcgcgctgga actacctggc ggcctggggc tatgcgctga
     8281 aacaaccggg cggcaacgaa gccaaggcgc gtgactttgt caaagcgctg ttcaaaaacg
     8341 tgccggtgct ggacaccggc gcccgcggct cgaccatgac ctttgtcgaa cgcggcatcg
     8401 gcgacgtgct gctggcctgg gaaaacgaag ccctgctggc caaaaaagaa ctcggcccgg
     8461 aaaaattcga catcatcgcc ccgccgctgt ccatcctggc ggaaccgcca gtggccgtgg
     8521 tggacaaaaa cgccgagaag aaaggcacgc tcaagcaagc caatgcgtat ttgaagttcc
     8581 tgtacacccc ggaagcgcaa accatcatcg ccgaaaacta cttccgcccg atcgacccga
     8641 ccgtggccag caaatacgcc gcgcaatacc ccaagctgaa actgttcacc attcaggatc
     8701 tgggtggctg gcgcaaagcg caaaccacgc acttcagcga tggcggcgtg tttgaccaga
     8761 tcaacgcccg ctgagcgctg cttcaagcgc aacaccctga atgcccgcct gcgcgggcat
     8821 gacggagaag tgggtgggta gccgttgata cagcatctgc ccgcaccaca cccacggccc
     8881 gccgtgctta gccgccgttc acccccgtgt ggcgcgccga gcatcgcaga aagtcaggga
     8941 gctttccgcc acggctaagc ggcggcgcag ccgacgcgcc attccgtggc ggccctgac
//
LOCUS       DAOPYX010000110         8993 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_9184_length_8993_cov_10.841352, whole genome shotgun sequence.
ACCESSION   DAOPYX010000110 DAOPYX010000000
VERSION     DAOPYX010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 8993)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8993)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8993
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9184_length_8993_cov_10.841352"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..534
                     /locus_tag="PKN55_07115"
     CDS             <1..534
                     /locus_tag="PKN55_07115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003746546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNX71048.1"
                     /translation="ADQARTIRIPVHMIETINKMNRISRQILQETGLEPDPAELATRM
                     EMPEEKIRKIMKISKEPISMETPIGDDDDSHLGDFIEDAVTMAPSDAAMYASLREATK
                     EVLDTLTPREAKVLRMRFGIEMNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPTR
                     SERLKSFLDNEQNGGGA"
     gene            634..1827
                     /locus_tag="PKN55_07120"
     CDS             634..1827
                     /locus_tag="PKN55_07120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012698416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HNX71049.1"
                     /translation="MTAAPQRSHFALALMLAALATLGPFSIDTFLPAMPTIARALGAS
                     PVAMQQALTVYLFCYGLMMLWHGGISDALGRKPVIVASTLVFTLASLGCALAQTLPQL
                     LLFRALQGLSGGAGLIVGRAVIRDTLDGPAAQKLMSHVTMLFSLSPAIAPVIGGWLFG
                     AFGWHSIFIFMALIGAALTLAAWLFLHESHPSHARQPLQARALLANYRLVAGQREFRL
                     LAVAVACNFSGFFLYIPSAPVFLMRHLGLNEQQFIYLFGPAVCGIMLGAFLSGRLAGR
                     RSPREIVSLGYSLMAIAAVSNLLYNLWLPPALPWSVLPLACYTVGMSLVAPTVTLFLM
                     DCFPSLRGATSSLQGFTQTLFSSLVAGVISPLLWDSPLTLALGMCGFLACGYGLLWRV
                     RRGRV"
     gene            complement(1951..3609)
                     /locus_tag="PKN55_07125"
     CDS             complement(1951..3609)
                     /locus_tag="PKN55_07125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014087156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid--CoA ligase"
                     /protein_id="HNX71050.1"
                     /translation="MSFTIAAHAAAQAYGYPLLIKQLLHTTLTQASQQEIVYADRFRY
                     TYADFYQRVQRLAQALTTLGVGMGDTVAVMDWDSHRYLESYFAVPMMGSVLFNVNVRL
                     SEDQIIYTMNHARPKVLLVNAEFLPVFNDIRAQLESVEHVILLNDGAPQDAAALGMAG
                     EYEALLAASPAQFDFPDFDEHTRATTFYTTGTTGLPKGVFFSHRQLVLHTLSAALGLA
                     SNPEHGRLHREDVYMPITPMFHVHAWGVPYIATLLGIKQVYPGRYLPESLLNLIAQEK
                     VTFSQCVPTILHMLLTHPRAKDVDLTGLKMVIGGSALTEGLAHTALARGMDVFVGYGM
                     SETCPIMSLSRLTREQLTQDAAAQIPLRIKTGQAIPLVDLRIVDDNMADLPHNGSRTG
                     EVVARAPWLTQGYLHNPAASDELWAGGYLHTNDIGMMDEHGVLQITDRLKDVIKTGGE
                     WISSLELESLLTRHPAVSECAVIAIPDAKWGERPLALVVLKPEQQASADELRAHLRDF
                     AEQGHISRWAVPEHIRFVDALARTSVGKLNKRALREQFVQQSQG"
     gene            complement(3679..4839)
                     /locus_tag="PKN55_07130"
     CDS             complement(3679..4839)
                     /locus_tag="PKN55_07130"
                     /inference="COORDINATES: protein motif:HMM:NF024996.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HNX71051.1"
                     /translation="MFHHTLRLSALALACASLGALADTTIYGKINLSLEHVAARGAGN
                     GQDILPMQRVTSNLSYLGFKGEEDLGDGLKTVWQVEQDINPDNCSGSCGGFANRNSFV
                     GLKGSWGRAIAGRYDMYWTSHIAGMDSRIINAGLAGSILSVFGTFGGYNPTPGNSKRG
                     TAALMGGRAVNVLRYDTPNLDGWTGSLSYATGETRGASGSPSAWQIESDYKKGQLVAN
                     LVYLHANDSNGSLTNTSGAAYDGMRTDAAKLVTAWRFDQGTLLGLGAEYLATRYPEGG
                     RVRRMAYAMHLGQSLSPAAYVGMTVGRAGRVNSSVAGAANTDDTGARFISMIASYNLS
                     KRTMVYAEFARLYNAANAGYYFVSTGNLNASGLSAGKGADPRSLMIGMNHTF"
     gene            complement(5271..>6788)
                     /locus_tag="PKN55_07135"
     CDS             complement(5271..>6788)
                     /locus_tag="PKN55_07135"
                     /inference="COORDINATES: protein motif:HMM:NF025214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNX71052.1"
                     /translation="TPAAAFEPTPMPAEAETDNNPQALAQGLAALAAQRYTEAWRKLR
                     QASRQQGDEVEVWLALGEACQGLGRLDEAAEAWRQALTLEPANWAAESALLRLSQLQP
                     ALSAAAAAERHLSAGATLEAHWPEPAAPTPRQAHDALRVGFIGPGLCDHPHAQVLLPL
                     WQALASSSLEVWAYHDAVQHDAITERLRAACAGWREVAGLDDDSLLAQIIADDIDILV
                     DLHGHQPGNRLAVFARQAAPLQVGWLGHADGSGLSRMHWRLTDAATHPAGVEAQHREQ
                     LWRLADGQYCHQPLWHVLQQRPSSDYAVRGAPALHRGHVTFGACLPAAQLNGEVIALW
                     AQVLHAVADSRLRLELPGLDASDSPLHVALSTQFAERGIGGHRLQLLPHDPSRLAVRA
                     HDIDIALDSFPVSVGLSACELLWMGVPVITLPGARPASRAAASLLTTLGCPQWIAHSK
                     VDYVRIAKTLASDLPQLNRTRLSLRVEMESSPLRQPQRFAEQVETALRGMWAARS"
     assembly_gap    6789..6888
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6889..7367)
                     /locus_tag="PKN55_07140"
     CDS             complement(<6889..7367)
                     /locus_tag="PKN55_07140"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNX71053.1"
                     /translation="MSEPTAPDLAAQLQAAIAHHQAGELAEAEQGYRAILAEQANHPD
                     AWHNLGILALQVEQHDTALELLRGVLQAYPQHGQFWISYLQALMRIGQFDLARAVLAQ
                     GRELGLDGEPVDAIARQLELLPGAAPDTPGEATSPNDTAAQTDTPTQEETPTAAETA"
     gene            complement(7492..8901)
                     /locus_tag="PKN55_07145"
     CDS             complement(7492..8901)
                     /locus_tag="PKN55_07145"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HNX71054.1"
                     /translation="MNHPEHVLAHLTFWRKEISQQLAEWIEHCDGAVAVDRHARVVWI
                     SKPYLEALGLTRPEDALGRHILELTPHSQMPEVLETGRPILLDIWTISERSFVISRVP
                     LKDEHGEVIGALGFVLYESSKHIKPLLAKFARLQSQLECTQSALLLARSNKYTLADMV
                     GHSSAMQQLKRAARRAARQDSTVLLLGETGTGKELLAHAIHAESARTGKPFVAINVAA
                     IPDSLLEAELFGAAAGAYTGADRKGRLGKIQLADGGTLFLDEIGDMPLNLQAKLLRVL
                     QEKEVEPLGSNRVCPVDLRVIAATSADLPRRVADGSFRADLYYRLNVLPLTLPPLRQR
                     LDDLPSLCEFILARLAEKTGSLPRDIDAAALALLREQRWPGNLRQLRNTLEQAVAMTD
                     GDTLGAAELRPFLPDPTPAAPPARTLADAVAATERQMIQAALVEQQGRVVDAARQLGV
                     SRATLYKKLAQYGLGGHEG"
BASE COUNT         1377 a         2844 c         3017 g         1655 t
ORIGIN      
        1 gccgaccagg cgcgcaccat ccgcattccg gtgcacatga tcgaaaccat caacaagatg
       61 aaccgcatca gccggcaaat cttgcaggaa accggcctgg agccggatcc ggccgagctg
      121 gcgacgcgca tggaaatgcc cgaggagaag atccgcaaga tcatgaaaat ctccaaggag
      181 ccgatctcca tggaaacccc gattggcgac gacgacgaca gccatctggg cgactttatc
      241 gaagacgccg tcaccatggc gccgtccgac gccgccatgt acgccagcct gcgcgaggcc
      301 accaaggaag tgctcgacac cctgaccccg cgcgaggcca aggtgctgcg catgcgcttt
      361 ggcatcgaaa tgaacaccga ccacaccctt gaggaagtcg gcaagcaatt cgacgtcacc
      421 cgcgagcgca ttcgccagat cgaagccaag gcgctgcgca agctgcgcca ccccacccgt
      481 tccgaacgct tgaagagctt cctcgacaac gaacagaacg gcggcggcgc ctgattccgg
      541 cgcttcctgt cacccccgat gccgcttgcg ccatcggggg ttttgctttt gtggcggccg
      601 ctgacagccg cccctcaccc gagaccccac tccatgaccg ccgccccgca acgcagtcac
      661 tttgccctgg cgctgatgct ggccgccctg gccaccctcg ggccgttttc catcgacacc
      721 tttttgccgg ccatgcccac catcgcccgc gcgctgggcg cctcgccagt ggccatgcaa
      781 caggcgctga ccgtctacct gttttgctac ggcctgatga tgctgtggca cggcgggatt
      841 tctgacgcgc tggggcgcaa gccggtgatt gtcgcctcca ccctggtgtt taccctggcc
      901 tcgctgggct gcgcgctggc gcaaaccctg ccgcagctct tgctgtttcg cgccctgcaa
      961 ggcctatccg gcggcgccgg cttgattgtt ggccgcgcgg tgatccgcga caccctggac
     1021 ggcccggccg cgcaaaagct gatgtcgcac gtcaccatgc tgttttccct gtcgccggcc
     1081 atcgccccgg tgattggcgg ctggctgttt ggcgcatttg gctggcactc gatttttatt
     1141 ttcatggcgc tgatcggcgc cgcgctcacc ctggccgcct ggctgtttct gcacgaaagc
     1201 caccccagcc acgcccgcca gccgctgcaa gcgcgcgccc tgctggccaa ttaccgcctg
     1261 gtggccggcc agcgcgaatt ccgcctgctc gccgtggcgg tggcgtgcaa tttcagcggg
     1321 ttttttctgt acatcccctc cgccccggtg tttctgatgc gccatctggg gctgaacgaa
     1381 cagcaattca tctacctgtt tggcccggcg gtgtgcggca tcatgctggg cgcgtttctc
     1441 tccggccgcc tggccggccg ccgcagcccg cgcgaaatcg tctcgctggg ctacagcctg
     1501 atggccatcg ccgccgtcag caacctgctg tacaacctgt ggctgccgcc cgccctgccc
     1561 tggagcgtgc tgccgctggc ctgctacacc gtcggcatgt cgctggtcgc ccccaccgtc
     1621 accctcttcc tgatggactg cttccccagc ctgcgcggcg ccacctcctc actgcaaggc
     1681 ttcacccaga cactgttctc cagcctggtg gccggggtga tctcgcccct gctgtgggat
     1741 tccccgctta ccctggcgct gggcatgtgc gggtttctgg cgtgtgggta tgggttgctg
     1801 tggcgggtgc ggcgggggcg ggtgtgaatc tgtagacagg caaaaaaaca cccccgctgt
     1861 cgcgggggcg caggccatgc ggaagcgccc gctccagcat gggggtaagc cggccgacgg
     1921 ggacggcggg ccggcaaccc aaggaggcaa ttagccctgg ctttgctgaa caaactgctc
     1981 gcgcagcgcg cgtttgttca gcttgcccac gctggtgcgc gccagcgcat cgacaaagcg
     2041 gatgtgctcg ggcaccgccc agcgcgagat atggccttgc tcggcaaagt cgcgcagatg
     2101 ggcgcgcagc tcgtcggcgc tggcttgctg ttcgggcttg agcaccacca gcgccagcgg
     2161 gcgttcgccc catttggcgt cggggatggc gatcaccgcg cattcgctga ccgccgggtg
     2221 gcgggtgagc agggattcca gctccagcga ggaaatccac tcgccgccgg ttttgatcac
     2281 gtctttcagg cggtcggtga tttgcagcac gccgtgctcg tccatcatgc cgatgtcgtt
     2341 ggtgtgcaaa tagccgccgg cccacagttc gtccgaggcg gccgggttgt gcagatagcc
     2401 ctgggtgagc cagggcgcgc gcgccaccac ttcgccggtg cggctgccgt tgtgcggcag
     2461 gtcggccatg ttgtcatcga caatgcgcag gtccaccagc gggatggcct ggccggtttt
     2521 gatgcgcagc ggaatttgcg cggccgcgtc ctgcgtcagt tgctcgcggg tcaggcggga
     2581 caagctcatg atcgggcagg tttccgacat gccgtagccg acaaacacat ccatgccgcg
     2641 cgccagcgca gtgtgcgcca gcccttcggt gagcgccgaa ccgccgatga ccattttcag
     2701 gccggtcaga tcaacatcct tggcgcgcgg gtgggtgagc agcatgtgca aaatggtggg
     2761 cacgcactgc gagaaggtga ctttttcttg tgcgatcagg ttcagcaggc tctcgggcag
     2821 gtagcggccg gggtaaacct gcttgatgcc cagcagggtg gcgatatacg gaacgcccca
     2881 ggcgtgcaca tggaacatcg gcgtaatcgg catgtacacg tcttcgcggt gcaggcggcc
     2941 atgctcgggg ttgctggcca ggcccagcgc ggcggacagg gtgtgcagca ccagttggcg
     3001 atggctgaaa aacaccccct tgggcaggcc ggtggtgccg gtggtgtaaa aggtggtggc
     3061 gcgggtgtgt tcgtcaaagt cggggaagtc aaactgcgcc gggctggcgg ccagcagcgc
     3121 ctcgtattcg ccggccatgc ccagcgcggc ggcgtcctgc ggcgcgccgt cgttgagcag
     3181 gatgacgtgt tccaccgatt ccagctgggc gcggatgtcg ttgaacaccg gcaggaattc
     3241 ggcattcacc agcagcacct tgggccgggc gtggttcatg gtgtagatga tctggtcttc
     3301 cgacaggcgc acattcacat tgaacagcac gctgcccatc atcggcacgg caaaatagct
     3361 ttccaggtag cgatggctgt cccagtccat caccgccacg gtgtcgccca tgcccacgcc
     3421 cagcgtggtc agcgcctggg ccagccgctg cacccgctgg taaaagtcgg cataggtgta
     3481 gcgaaagcgg tcggcgtaga caatttcttg ctggctggcc tgggtgagcg tggtgtgcag
     3541 cagctgcttg atcagcagcg gatagccata ggcctgggcg gcggcgtggg cggcgatggt
     3601 gaagctcatg tcaatctcct ctggtgttgt tggccggcgg cgcgccgccg gtttgtttgt
     3661 tgtgggggtg aatggcgctt agaaagtgtg gttcatgcca atcatcaacg accgcggatc
     3721 cgcccccttg ccggcgctca ggcccgaggc attcaggttg ccggtggaga caaagtaata
     3781 acccgcgttg gcggcgttat acagccgggc aaactcggcg tagaccatgg tgcgcttgga
     3841 gaggttatag ctggcaatca tgctgatgaa gcgcgcgcca gtgtcgtcgg tgttggcggc
     3901 gcccgccacc gagctattca cccgaccagc gcggcccacc gtcatgccca catacgcggc
     3961 gggcgacagc gattgcccca ggtgcatggc gtaggccatg cgccgcacgc ggccgccttc
     4021 cgggtagcgg gtggccaggt attccgcgcc caggcccagc agggtgccct ggtcaaagcg
     4081 ccaggcggtg accagcttgg cggcgtcggt gcgcatgccg tcgtaggcgg cgccgctggt
     4141 gttggtcagg ctgccgttgc tgtcattggc gtgcagatac accagattgg ccaccaactg
     4201 gccttttttg tagtcggatt cgatttgcca ggcgctgggg ctgccgctgg cgccgcgggt
     4261 ttcgccggtg gcgtagctca ggctgcccgt ccagccgtcc agattggggg tgtcgtagcg
     4321 cagcacattc accgcgcggc cgcccatcag cgcggcggtg ccgcgtttgc tgttgccggg
     4381 cgtggggttg tagccgccaa aggtgccaaa caccgacaga atcgagccgg ccaggccggc
     4441 gttgatgatg cggctgtcca tcccggcgat atgcgaagtc cagtacatat cgtagcgccc
     4501 ggcaatcgcc cggccccagc tgcccttcag gccaacaaaa gaattgcggt tggcaaagcc
     4561 gccgcagctg cccgaacagt tgtccgggtt gatgtcctgc tcgacctgcc agacggtttt
     4621 caggccgtcg ccgaggtctt cttcgccctt gaagcccaga taggacaaat tgctggtgac
     4681 ccgctgcatg ggcaggatgt cctgaccatt gccggcgccg cgcgcggcga cgtgctccag
     4741 cgagaggttg atcttgccgt aaatggtggt gtcggccagg gcgcccaggc tggcgcacgc
     4801 cagcgccagc gcgctcaggc gcagagtgtg atgaaacatg tgtttctccc ttgtagttgt
     4861 tgtgccgcac gtctgcgcgc gcgctccaca cacccgccgg tctgcgccat tgggctgtcg
     4921 cgtggggcgg cggccggggt ggctgcctgc gctcgcgcgg cgggccggca ttgcaagagc
     4981 ggggccaagc gggttaatgg gctgatctac aagggaaatt caaaaaaatc agtgtgtttt
     5041 gcccgcaaaa gcgggcgatt tgcgccagaa actgtccact gagttggaat cttggccgag
     5101 ggtggcgcta actgattgtt ttatattgtg ataaattgtc tcgtttttta gacgggtttg
     5161 tctgggattc gagacaatgg cttgcgctgg ggttggccac cttgcttgcc atcaaaaaac
     5221 acgcccccga cagcggctgt cgggggcgtg ttgtgtgcaa tgagcggcgc ttagctgcgc
     5281 gccgcccaca tgccacgcaa ggccgtctcc acctgttcgg caaagcgctg cggctggcgc
     5341 agcgggctgc tttccatctc cacccgcagg ctcaaacggg tgcggttcag ttgcggcaaa
     5401 tcgctggcca gggttttggc gatgcgtaca tagtccacct tgctgtgggc aatccactgc
     5461 gggcagccca gcgtggtcag caggctggcg gccgcgcgcg aggccggccg ggcgccgggc
     5521 agggtgatca ctggcacgcc catccacagc agctcgcacg ccgacaggcc cacgctcacc
     5581 ggaaagctat ccagcgcaat atcaatatcg tgggcgcgca ccgccagccg gctggggtcg
     5641 tgcggcaaca gttgcagacg gtggccgcca atgccgcgct cggcaaactg ggtgctcagc
     5701 gccacatgca gcgggctgtc gctggcgtcc aggccgggca gttccaggcg caggcgtgaa
     5761 tcggccaccg catgcagaac ctgtgcccac agcgcaatca cttcgccatt cagttgcgcg
     5821 gccggcaggc aggcgccaaa ggtcacatgg ccgcgatgca gcgccggtgc gccgcgcacg
     5881 gcgtaatcgc tggacgggcg ctgttgcagc acatgccaca gcggttggtg gcaatactgg
     5941 ccatcggcca gccgccacag ctgttcgcgg tgctgcgcct cgacgccggc cgggtgggtg
     6001 gcggcgtcgg tcagtcgcca gtgcatgcgg ctgagcccgc taccgtcggc atgacccagc
     6061 cagcccactt gcagcggcgc ggcctggcgg gcaaacaccg ccagccgatt gcccggctga
     6121 tggccgtgca aatccaccag gatgtcgatg tcgtcggcga tgatttgcgc cagcaggctg
     6181 tcgtcgtcca gcccggctac ctcgcgccag ccggcgcagg cggcgcgcag gcgctcggtg
     6241 atggcgtcgt gttgcacggc atcgtgatag gcccagactt ccagcgagct gctggccagc
     6301 gcttgccaca ggggcaacag cacctgggcg tgcgggtggt cgcacaggcc agggccgata
     6361 aagcccacgc gcaaggcgtc gtgtgcctgg cgcggtgtcg gcgcggcggg ctcgggccag
     6421 tgggcttcca gtgtggcgcc agcgctgagg tgacgctcgg cggcagcggc ggccgacagc
     6481 gccggttgca gctggctcag acgtaacagt gcggattctg ccgcccagtt ggcgggctcc
     6541 agggtcagcg cctgccgcca ggcctcggcg gcttcatcca gccggcccag cccctggcat
     6601 gcctcgccca gcgccagcca gacttccact tcgtctccct gctggcggct ggcctgacgc
     6661 agtttgcgcc aggcttcggt gtaacgctgt gcggccagcg cggccagccc ctgcgccaat
     6721 gcttggggat tattgtctgt ctcggcctct gctggcatcg gtgtgggctc aaaggcggcg
     6781 gctggcgtnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnntc tgcggtttcc
     6901 gctgccgtcg gcgtttcttc ctgagtcggg gtgtcggtct gcgctgccgt atcgtttggc
     6961 gaagtggctt cgcctggcgt gtctggtgcg gcgccgggca aaagctccag ttggcgggcg
     7021 atggcgtcca ccggttcgcc gtccaggccc aattcgcggc cttgtgccag cacggcgcgg
     7081 gccaggtcga actggccgat gcgcatcagc gcctgcagat agctgatcca gaactgacca
     7141 tgctgagggt aggcttgcag cacgccgcgc agcagttcca gcgcggtgtc gtgctgctcc
     7201 acttgcaggg cgagaatgcc caggttgtgc caggcgtcgg ggtggttggc ttgttcggcc
     7261 agaatggcgc ggtaaccttg ctcggcctcg gccagttctc ccgcttggtg atgggcgatg
     7321 gcggcctgga gttgggcggc aagatcgggg gcggtgggct cggacatgac ggcaatcaat
     7381 caagatggcc cgctgtgcgg gatgacgcgg gcgctaggta aaactcctga ttattggtgg
     7441 cgacaggggg cttgtccagc gtttgcagcg gagttttcgc ccggcctggc gtcagccttc
     7501 gtgtccgccc agcccgtact gcgccagttt tttatacagc gtggcgcgtg agacgcccaa
     7561 ttggcgggcg gcgtccacca cccggccttg ttgctcgacc agcgcggcct gaatcatctg
     7621 ccgctcggtg gcggccaccg cgtcggccag ggtgcgcgca ggcggcgcgg ccggggtggg
     7681 gtcgggcagg aaggggcgca gctcggcggc gcccagggtg tcgccgtcgg tcatcgccac
     7741 ggcttgttcc agggtgttgc gcaattgccg cagattgccc ggccagcgtt gttcgcgcag
     7801 cagcgccagc gcggcggcgt cgatgtcgcg cggcagcgag ccggtttttt ccgccagccg
     7861 ggccaggatg aattcgcaca ggctgggcag gtcgtccagc cgctggcgca gcggcggcag
     7921 ggtgagcggc agcacattca gccgatagta caaatccgcc cgaaaactgc cgtcggccac
     7981 ccggcgcggc aggtcggcgc tggtggcggc aatcacccgc aaatccaccg ggcacacccg
     8041 attgctgccc agcggctcga cttctttttc ttgcagcacg cgcagcagct tggcttgcag
     8101 gttcagcggc atatcgccga tttcgtccag aaacagcgtg ccgccgtcgg ccagctggat
     8161 tttgcccaga cggcccttgc ggtcggcgcc ggtgtaggcg ccggcggcgg cgccaaataa
     8221 ttcggcttcc agcaggctat cgggaatggc ggcgacattg atggcgacaa acggcttgcc
     8281 ggtgcgggcg gattcggcat ggatggcgtg cgccagcagc tctttgccgg tgccggtttc
     8341 gcccagcagc agcacggtgc tgtcctgacg ggcggcgcgg cgggcggcgc gcttgagctg
     8401 ctgcatggcg ctggagtggc ccaccatatc ggccagggtg tatttattgc tgcgcgccag
     8461 cagcagcgcg ctttgcgtgc attccagctg gctttgcagg cgggcgaatt tggccagcag
     8521 cggcttgatg tgtttgctgc tttcatacaa cacaaagccc agcgcgccaa tcacctcgcc
     8581 gtgctcgtcc ttgagcggca cccgcgaaat cacaaagctg cgctcgctga ttgtccagat
     8641 gtccagcagg atggggcggc cggtttcgag cacttccggc atttgcgaat gcggggtgag
     8701 ctccagaata tgccggccca gcgcgtcttc cgggcgggtc aggcccaggg cttccaggta
     8761 gggtttgctg atccacacca cacgggcgtg acgatccacc gccaccgcgc cgtcgcagtg
     8821 ctcgatccat tcggccaatt gctgggagat ttctttgcgc cagaaggtca ggtgcgccag
     8881 cacatgttcg gggtggttca tcggggcggc tcgcaaacag atgtttgctc gggattgtag
     8941 cgcgacgggg cgacgcaggc gcgctgttgc acgcgcgcgt cgcaccagga tgg
//
LOCUS       DAOPYX010000111         8948 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_9259_length_8948_cov_11.691555, whole genome shotgun sequence.
ACCESSION   DAOPYX010000111 DAOPYX010000000
VERSION     DAOPYX010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 8948)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8948)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8948
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9259_length_8948_cov_11.691555"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1426)
                     /gene="thiC"
                     /locus_tag="PKN55_07150"
     CDS             complement(<1..1426)
                     /gene="thiC"
                     /locus_tag="PKN55_07150"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011133790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HNX71055.1"
                     /translation="MNAPHTPFNQTAEVDQAAVQPLPNSKKIYVTGSRPDIRVPMREI
                     SQADTPTSFGGEANPPITVYDTSGPYTDPAVRIDVRSGLAALRAAWIEERQDTELLAD
                     LSSEYGRQRAADEKLDPLRFNLTRAPRRAKAGANVSQMHYARRGIITPEMEYVAIREN
                     LRRREYLDSLKASGPQGEKMARLLTRQHPGQRFGAGIPEEITPEFVRDEIARGRAIIP
                     ANINHPESEPMIIGRNFLVKINGNIGNSAVTSSIGEEVEKMTWGIRWGADTIMDLSTG
                     KNIHETREWIIRNSPVPIGTVPIYQALEKVGGKAEDLTWEIFKDTLIEQAEQGVDYFT
                     IHAGVLLRYVPMTAERMTGIVSRGGSIMAKWCLAHHQENFLYTHFEEICEIMKAYDVA
                     FSLGDGLRPGSIYDANDDAQLGELKTLGELTQIAWKHDVQVMIEGPGHVPMQLIKENM
                     DKELEWCDEAPFYTLGPLTTDIAPG"
     regulatory      complement(1473..1578)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            1654..2313
                     /locus_tag="PKN55_07155"
     CDS             1654..2313
                     /locus_tag="PKN55_07155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017507729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HNX71056.1"
                     /translation="MDFEKARFNMVEQQIRPWDVLNTQLLDLLFHVKRENFVADNQKA
                     IAFVDTELPLPNGSRMLQPKMEARILQELNIAADEKILEIGTGSGYLTALLASVGKHV
                     YSLDIDANMTALAKANLARAGIQNVTLVTANGVGGLPTNAPFDVIVVGGALPSVPEEL
                     TSQLAVGGRMLVVVGDLAPLAATLVERVKEGPSGLRSTVLFETHLDALQGVGQPERFV
                     F"
     gene            2313..2645
                     /locus_tag="PKN55_07160"
     CDS             2313..2645
                     /locus_tag="PKN55_07160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003786578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNX71057.1"
                     /translation="MGAVQEITARALADWLNDASRPAPHLLDVREEWEYAHCHLANSQ
                     LMPMHTVPLHMNSLPDDAPLVVICHHGVRSYQVAAYLLNAGFAEVFSLAGGVAAWADQ
                     VDPAMPRY"
     gene            2748..3476
                     /gene="ung"
                     /locus_tag="PKN55_07165"
     CDS             2748..3476
                     /gene="ung"
                     /locus_tag="PKN55_07165"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: protein motif:HMM:NF003588.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HNX71058.1"
                     /translation="MSDLSFLAPAYANLDADWRRALSAVWTQPKWRALDHALQQRHAA
                     GAQIYPQRETVFHALNLTPPDQVKVVILGQDPYHGEGEAHGLAFSVPAGVKIPPSLRN
                     IFAEQQQDLGAPTPRHGDLSAWARQGVLLLNSVLTVEKDCANSHRKLGWEVLTHAVIK
                     HLAERDTPTVFLLWGSAAQKLGAPAAANPQHGVLNSVHPSPLSAYRGFFGCQHFSQAN
                     AFLRTHGRGEIDWRLPDGGSGSLF"
     gene            complement(3667..4632)
                     /locus_tag="PKN55_07170"
     CDS             complement(3667..4632)
                     /locus_tag="PKN55_07170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014088789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase"
                     /protein_id="HNX71059.1"
                     /translation="MKVLLTGAAGFIGRATAEALLKAGHQVVAVDNLNDYYAVSLKHA
                     RLATLREQPGLQFYPLDVADWPALDALFAEQRPDAVIHLAAQAGVRYSIQNPHAYAQS
                     NLLGMTHILEACRQHPVQHLVYASSSSVYGKNAKAPFAEDDRTDQPVSFYAASKKANE
                     VMAASYSHLYQLPATGLRFFTVYGPWGRPDMAPWLFTEAILRGEPIKVFNHGRLQRDF
                     TYIDDIVEGILRVLAQPPQGDCPHTLYNIGNHQPVELMTFISTIEQALGREAEKIYLP
                     MQDGDVPITYADTQKLRDAVGFSPDTPLADGMRRFVDWYRAYHQC"
     gene            complement(4633..5382)
                     /locus_tag="PKN55_07175"
     CDS             complement(4633..5382)
                     /locus_tag="PKN55_07175"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018152622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNX71060.1"
                     /translation="MQLSLVLITLNAEATLAKCLASCDFIDDIVVVDSGSQDNTMAIA
                     EQFGARVIQQTWLGFGKQKQFAVQQAQYDWVLCLDADEWLSDQLKHSIQQLRPDHKTW
                     AFTMPRCNKFMGQFLKHGEGYPDWSLRLFHRQHAQWSDDVVHETVQYQGLIGKLQGDL
                     MHESGEVLGEYLRKQNRYTDLQAEALLAKQKSPSASKMLLSPLVRFIKFYLVRQGWRD
                     GLPGFVHISIGCMNSMMKYAKLYELQRKSRQ"
     gene            5878..6849
                     /gene="waaC"
                     /locus_tag="PKN55_07180"
     CDS             5878..6849
                     /gene="waaC"
                     /locus_tag="PKN55_07180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014088786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase I"
                     /protein_id="HNX71061.1"
                     /translation="MSRLLLVRMSSMGDLIHTLPALTDLACRFPDLRVDWLAEEAFTD
                     IPRLHPAVAQVLPIAWRRWRKQLWSPSVWRQMRACYQQLNATPYDLVLDAQGLLKSAL
                     AARLVRRTPLGGYAADSIREPLASRFYHKTYHVSRTLSAITRNRLLLGLAFGYQPDLA
                     HIRFGIQPGARPAWLHAERYAVLLTATSRAEKEWPEAHWLRLGAALHAQGVAVVWPWG
                     NAVEQARALRLADGLPGSQAAPKLRLAEAAGLLGHACAVVGVDTGLTHLANALDVPLV
                     ALYTDTDPALTGVVPSARAMNLGGVGQCPDVEAVLAALARCQAGAAA"
     gene            6846..8108
                     /gene="waaA"
                     /locus_tag="PKN55_07185"
     CDS             6846..8108
                     /gene="waaA"
                     /locus_tag="PKN55_07185"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014088785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HNX71062.1"
                     /translation="MIWPLLYAALWKLAPPLIGHYLRRRARKQPEYLQHWDERWARGE
                     PRADDAHGAIWVHAVSVGETRAAAPLVAALRARWPDKPLLITQMTPTGRETALSLYPD
                     ACVRYLPYDAPDAVARFLGRYQPMFGVLMETELWPHLIAGCRAHGVPLFLANARLSER
                     SARGYRRAAGLIGPALASLDGVAAQSEDDAARLRALGARQVAVCGNLKFDITPPPQAA
                     ALAAQLRQWRGERPLWMAASTREGEEALLLDALPADSPAVLLLTPRHPQRFAEVAELL
                     RARGIPFQRRSDNTALRADTRVWLGDSMGEMFAYYQAVDLAFIGGSLLPLGGQNLIEP
                     AAVGCPVLIGPSTFNFAEVTRLALAEGAARQVADADELHRTVATLLADPDARQRMGAA
                     GLAFAQRHRGATARTLAHLLGCVGEVVD"
     gene            complement(8206..>8948)
                     /locus_tag="PKN55_07190"
     CDS             complement(8206..>8948)
                     /locus_tag="PKN55_07190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX71063.1"
                     /translation="RETVCVIDARHLPELLPGAGEISGVFSLQGWLGQKIEGNFGDKI
                     FIFLFFTALATIIYLPALLYRWNLKASAWLWGPVALLLSPVLWANDETMRHKTAYWTT
                     RAVQSVLWSVWVGFVGWLLAAYLPDDVRALFPHWVTVVYGHFPLPTLGLRYGLLVLLA
                     LSLLGLLEAAYRVRAVHGKALEGDFEKGSEELKAELRRLAIPVSRWLRVNLVLMTFNI
                     WAFAWQWAVQYWPNQLQHITWPWLRPWL"
BASE COUNT         1702 a         2736 c         2720 g         1790 t
ORIGIN      
        1 agccgggggc gatgtcggtg gtcagcgggc caagggtgta gaacggcgct tcgtcgcacc
       61 attccagctc tttgtccata ttctctttga tcagctgcat cggcacatgg ccggggcctt
      121 cgatcatcac ctgcacatcg tgcttccagg caatctgggt gagctcgccc agggttttca
      181 gctcgcccag ctgggcgtcg tcgttggcgt cgtaaatcga gccaggacgc aggccatcgc
      241 ccaggctgaa ggccacatcg taggccttca tgatctcgca gatttcctca aaatgggtgt
      301 agaggaagtt ttcttgatgg tgggccagac accatttggc catgatcgag ccgccccggc
      361 tgacgatgcc ggtcatgcgc tcggcagtca tcggcacata gcgcagcagc acgccagcgt
      421 ggatggtgaa gtaatccacg ccttgttcgg cttgctcgat cagcgtgtct ttgaagattt
      481 cccaggtcag gtcttcggcc ttgccgccga ctttttccag cgcctgataa atcggcaccg
      541 tgccaatcgg caccgggctg ttgcgaatga tccactcgcg ggtttcatgg atgtttttgc
      601 cagtggacaa atccatgatg gtgtcggcgc cccagcggat gccccaggtc attttttcga
      661 cttcttcgcc aatgctggaa gtcaccgcgc tattgccgat attgccgttg atcttcacca
      721 ggaagttgcg gccgatgatc atcggctcgg attccgggtg gttgatattg gccgggatga
      781 tggcgcggcc acgggcgatt tcgtcgcgga cgaattccgg ggtgatttct tccggaatgc
      841 cggcgccaaa gcgttggccg gggtgttggc gggtgagcag gcgcgccatt ttttcgcctt
      901 gcgggccgct ggccttcagg ctgtccaggt attcacggcg acgcaggttt tcccgaatcg
      961 ccacatattc catttcgggg gtgatgatgc cacggcgggc gtaatgcatc tggctgacat
     1021 tggccccggc cttggcgcgg cgcggcgcgc gggtgaggtt aaagcgcagc gggtcgagct
     1081 tttcgtcggc ggcgcgctgg cggccgtatt cgctggacaa atccgccagc agctcggtgt
     1141 cctggcgttc ttcaatccag gcggcgcgca gcgcagccag gccagagcgc acgtcgatgc
     1201 gcacggccgg gtcggtgtac gggccggagg tgtcgtacac ggtaatcggc gggttggctt
     1261 cgccgccaaa tgaggtgggg gtgtcggcct ggctgatttc acgcatcggc acccggatat
     1321 ccgggcggct gccagtcacg taaatctttt tcgagttggg cagcggctgg acagcggcct
     1381 ggtcaacctc ggcggtctgg ttgaaggggg tgtgcggtgc gttcatggcg gattttcctc
     1441 gtgttcggca aaaaaacggg aaaccgcaca atacgtgcgc catcccgctt ccctacgccg
     1501 atatgagccg gatcaggttc aaagggtaac tctcattccc ggggccggca ggccaacggg
     1561 aaacccctag cggacagtgc tgtgaagcta aagcaaatat gggataattg caaggtactg
     1621 tcatggctcg ataaacctcg ttcggagtgg tcaatggatt ttgaaaaagc ccggttcaac
     1681 atggtcgaac aacagattcg cccgtgggat gtgctcaata cgcagctgct ggatctgctg
     1741 tttcacgtga aacgcgaaaa cttcgtcgcc gacaatcaaa aggccatcgc ctttgtcgat
     1801 accgaactgc ccctgcccaa cggcagccgc atgctgcaac cgaagatgga agcgcgcatc
     1861 ctgcaagagc tgaacattgc cgcagacgaa aaaatcctgg aaatcggcac cggcagcggt
     1921 tatctgaccg ccctgctggc cagcgtgggc aaacatgtct acagcctgga catcgacgcc
     1981 aatatgaccg cgctggccaa ggccaatctg gcgcgcgccg gcatccagaa tgtcaccctg
     2041 gtgaccgcca atggcgtggg cggcttgccg accaacgcgc cgtttgacgt catcgtggtg
     2101 ggcggcgcct tgcccagcgt gccggaagaa ctcaccagcc agctggcggt gggcggccgc
     2161 atgctggtgg tggtgggcga tctggccccg ctggccgcca ccctggtcga gcgcgtcaaa
     2221 gaaggcccca gcggcctgcg cagcactgtg ctgtttgaaa cccatctgga cgcgctgcaa
     2281 ggcgtgggcc agccggaacg ctttgtgttc tgatgggcgc cgttcaagaa atcaccgccc
     2341 gcgccctggc agactggctg aacgacgcca gccgcccggc gccgcatctg ttggatgtgc
     2401 gcgaagaatg ggaatacgcc cattgtcacc tggccaatag ccagctgatg ccgatgcaca
     2461 ctgtgccgct gcacatgaac agcctgccgg acgatgcgcc gctggtggtg atctgccacc
     2521 atggcgtgcg cagctatcag gtggcggcgt atttgctcaa cgctggcttt gctgaggtat
     2581 tcagcctggc tggcggtgtg gccgcctggg ccgatcaggt ggatccggcc atgccgcgct
     2641 actgatcacc cagcgtgatt tgccccgcac aacggccagc ccctgctggc cgttgtgcat
     2701 tcatccccag cttatccaca gacgcggcgc ccgccgccca gccgaccatg tccgacctca
     2761 gctttcttgc ccccgcttac gcaaacctag acgcagactg gcgccgcgcc ttgtctgcgg
     2821 tgtggacgca gccaaaatgg cgcgcgctgg accacgcttt gcagcagcgc cacgccgctg
     2881 gcgcgcaaat ttatccacag cgcgaaaccg tgtttcacgc gctcaacctc accccgcccg
     2941 atcaggtgaa agtggtcatt ctcggccaag acccttacca cggcgaaggc gaggcgcacg
     3001 gcctggcctt ctcggtgccg gccggggtga aaatcccgcc ctcgctgcgc aatatctttg
     3061 ccgaacaaca acaagacctg ggcgcaccca cgccacgcca cggcgacctg agcgcctggg
     3121 cgcggcaagg cgtgctgttg ctgaacagtg tgctgaccgt agaaaaagac tgcgccaaca
     3181 gccaccgcaa actgggctgg gaggtgctca cccacgcggt gatcaaacac ctggccgagc
     3241 gcgacacccc cacggtgttt ttgctgtggg gcagcgccgc tcaaaaactg ggcgcgcccg
     3301 ccgcagccaa tccgcagcat ggtgtgctga acagcgtgca tccgtcgcca ttgtcggcgt
     3361 atcgcggctt ttttggctgc cagcattttt cgcaggccaa tgcctttttg cgcacccatg
     3421 ggcgtgggga gattgattgg cgtttgccag atggcggcag cggaagctta ttttgaccgc
     3481 agatgcgtga taaaaaagcc ggcgaatgga tattcaccgg cttttttatt gggcggaatg
     3541 ctccacgtgg aacattcgcc gtgtctattt tttacactca gcgccgctca gcaaacccag
     3601 cgcagcgacc ttggcaggcg tgtgaccgca gacagtgtgg gcagtacggc aaggtcgtga
     3661 gcggttctaa cactggtgat acgcccgata ccaatcaaca aaccgccgca tgccgtcagc
     3721 caagggcgta tccggcgaaa acccgacagc gtcgcgcagt ttttgcgtat cggcgtaggt
     3781 aatcggcaca tcgccatctt gcatgggcag gtagattttt tctgcttcgc gccccagcgc
     3841 ctgttcgatg gtgctgatga aagtcatcaa ttccaccggc tggtgattgc caatattgta
     3901 cagcgtgtgc gggcaatccc cctgcggcgg ctgcgccagc acgcgcagga tgccttcgac
     3961 aatatcgtcg atataggtga aatcgcgctg caaacggccg tgattgaaca ccttgatcgg
     4021 ctcaccgcgc aggatggctt cggtaaacag ccacggcgcc atatcgggcc gaccccaggg
     4081 gccatacacg gtaaaaaagc gcaaacccgt ggccggcagt tgatacagat ggctgtagct
     4141 ggcggccatc acttcgttgg cttttttgct ggcggcgtaa aaactcaccg gctggtcggt
     4201 gcggtcatct tcggcaaacg gcgctttggc gtttttgccg tacaccgacg acgagctggc
     4261 gtacaccaga tgctgcaccg gatgttggcg gcaggcttcc agaatatggg tcatgcccag
     4321 cagattggac tgtgcataag catgcggatt ctggatcgag tagcgcaccc cggcttgcgc
     4381 cgccaggtga atcaccgcat ccgggcgctg ttcggcaaac agggcatcca gcgccggcca
     4441 gtcggccacg tccagtggat aaaattgcag gccgggctgt tcacgcaagg tggccagccg
     4501 ggcgtgcttg aggctgacgg cgtaatagtc gttgagatta tccacagcca ccacctggtg
     4561 gccggccttg agcagcgcct cagcagtggc gcggccgata aatccggcag cgccggtgag
     4621 caatactttc atttactggc gactcttgcg ttgtaattca taaagcttgg cgtatttcat
     4681 catgctattc atgcagccaa tgctgatatg cacaaatccg ggcaatccat ctcgccatcc
     4741 ctggcgaacc aaataaaact tgataaagcg caccaagggg ctgagcagca ttttactggc
     4801 agacgggctt ttttgtttgg ctaataatgc ctcggcttgt aaatctgtgt atcgattctg
     4861 tttgcgtaaa tattcaccca gcacctcgcc ggattcatgc atcaaatcac cctgtaactt
     4921 gccgataagt ccttgatatt gcacagtttc gtgcacaaca tcatctgacc attgcgcatg
     4981 ctggcggtga aacagccgca agctccaatc aggatagcct tcgccgtgtt ttaaaaactg
     5041 ccccatgaat ttattacatc tgggcatggt gaaggcccag gttttatggt cagggcgcag
     5101 ctgttgaata ctgtgtttta gctgatctga taaccattca tctgcatcca aacataatac
     5161 ccaatcatat tgagcttgct gaacagcaaa ttgtttttgt ttgccaaacc ctagccaggt
     5221 ttgctgaatg acacgggcgc caaattgttc agcgattgcc attgtattgt cttggctgcc
     5281 ggaatcgacc accacaatat cgtcaataaa gtcgcagctg gccagacatt tggccaaggt
     5341 ggcttcggca ttcagggtga taagcaccag agacagttgc atgacttgtc ttaataattg
     5401 aatacaaatt taaaaggacg atgaaacaat agtccggcaa gttctgtgga taacgctgtt
     5461 ttacattgat aaatcaacag attacgttgt ttgcgcttta ccggatgacc caccttgtct
     5521 gatgttggcg cattgtggac aactttgccg cttgggggtt ttgctgagtt atccacagct
     5581 gtctgacggt ctgtcggcaa agcgcggtgg acagttattt tgcctgcaat cgctttcaaa
     5641 cacgatccca ttttaaaaaa agacgatgaa acaatacttg gcaaaaactg tggataacat
     5701 ggtaaatctg tataaaaatc atcgctttgc cttgtgtatg ggcttggggt aagttctacg
     5761 gcgggccggt ggtgagtttg tggataagtt ttccacaggt ggaaaacctt gagttatcca
     5821 cagctggccc cggggatttc gcttggttaa cccccaggcc gcccgtacaa tcgcggcatg
     5881 tcacgacttt tgcttgttcg catgtcgtcc atgggcgact tgatccacac cttgcctgcc
     5941 ttgaccgatc tggcctgccg ctttccggat ttgcgcgtgg attggttggc ggaagaggcg
     6001 tttaccgata ttccccgtct gcacccggcg gtggcccagg tgttgccgat tgcctggcgg
     6061 cgctggcgca aacagctgtg gagcccgtcg gtgtggcggc agatgcgcgc ctgttatcaa
     6121 cagctcaacg ccacgccgta cgatctggtg ttggatgccc aaggcttgct gaaaagcgcg
     6181 ctggccgcgc gcttggtgcg gcgcacgccg ctgggcggct atgccgccga cagcatccgc
     6241 gagccgctgg ccagccgctt ctatcacaag acctatcacg tcagccgcac gctgtcggcc
     6301 atcacccgca accgcctgtt gctgggcctg gcctttggct atcagcctga tctggcgcac
     6361 atccgctttg gcattcagcc cggcgcgcgc ccggcctggc tgcatgccga gcgctacgcc
     6421 gtgctgctga ccgccacctc gcgcgcagaa aaagaatggc ccgaggcgca ctggctgcgc
     6481 ctgggcgccg ccttgcacgc ccagggggtg gccgtggtgt ggccgtgggg caatgccgtc
     6541 gagcaggcgc gcgccttgcg tctggccgat ggcctgcccg gcagccaggc cgcgcccaag
     6601 ctgcgcctgg ccgaagccgc cggcctgctg ggccacgcct gcgccgtggt gggggtggac
     6661 accgggctga cccatctggc caatgccctg gatgtgccct tggtggcgct ttacaccgac
     6721 accgatccgg ccttgacggg cgtggtcccg tcggcgcggg cgatgaatct gggtggggtg
     6781 gggcaatgcc cggatgtcga ggcggtgctg gcggcgctgg cgcgctgcca ggctggggcg
     6841 gcggcatgat ctggccgctg ctgtacgcgg cgctgtggaa gctggcgccg cccttgattg
     6901 gccattacct gcgccgacgc gcgcgcaagc agccggaata cctgcaacac tgggatgagc
     6961 gctgggcgcg cggcgagccg cgcgcagacg acgcccacgg cgccatctgg gtgcatgcgg
     7021 tgtcggtggg ggaaacccgc gccgccgcgc cgctggtggc cgctttgcgc gcgcgctggc
     7081 ccgacaaacc cctgttgatc acacaaatga cccccacggg ccgggaaacc gccctcagct
     7141 tgtatcccga cgcctgcgtg cgctacctgc cctacgacgc cccggacgcc gtggcgcgct
     7201 ttctaggccg ctatcagccg atgtttggcg tgctgatgga aaccgaactg tggccgcacc
     7261 tgattgccgg ctgtcgcgcg cacggcgtgc cgctgtttct ggccaatgcc cgtttgtctg
     7321 aacgttcggc gcgcggctat cggcgcgcag ccgggttgat tggcccggcg ctggccagcc
     7381 tggacggcgt ggcggcgcaa agcgaagacg acgccgcccg cctgcgcgcg ctgggcgccc
     7441 gccaggtggc ggtgtgcggc aatctgaaat ttgacatcac cccgccgcca caagccgccg
     7501 cgctggccgc ccagctgcgc caatggcgcg gagaacgccc gctgtggatg gcggccagca
     7561 cccgcgaggg cgaagaagcc ctgctgctgg acgccctgcc cgccgacagc ccggcggtgc
     7621 tgctgctcac cccgcgccac ccgcagcgct ttgccgaagt ggccgagctg ttgcgcgcgc
     7681 gcggcattcc gtttcagcgc cgcagcgaca acaccgccct gcgcgccgac acccgcgtct
     7741 ggctgggcga cagcatgggc gaaatgtttg cctactatca ggccgtcgat ctggccttta
     7801 tcggcggcag cctgctgccg ctgggcgggc aaaacctgat cgagcccgcc gccgtgggct
     7861 gcccggtgct gattggcccc tccaccttca actttgccga agtcacccgc ctggccctgg
     7921 ccgaaggcgc cgcccgccag gtggccgacg ctgacgaact gcaccgcacc gtcgccaccc
     7981 tgctggccga ccccgacgcc cgccagcgca tgggcgccgc cggcctggcc ttcgcccaac
     8041 gccaccgggg cgcgacagcg cgcaccctgg cgcatttatt ggggtgtgtg ggggaagtgg
     8101 tggattgagg gatcacgcgc ggcgcacgag cccacgctga actggcgggc gcgcctggtg
     8161 tggcaaggcc gcgcaacaac gccaggacag tgtggcgagt ggcgtttaca gccaaggccg
     8221 cagccacggc caagtgatgt gttgcaactg atttggccaa tattgcacag cccactgcca
     8281 ggcaaacgcc cagatattga acgtcatcaa caccagattt acccgcagcc agcggctcac
     8341 cgggattgcc aggcggcgta actcggcttt caattcttcg gatccttttt caaaatcccc
     8401 ttccagtgcc ttgccatgca ccgcccgaac ccggtaagcc gcttcgagca agccaagcag
     8461 agacagcgcc agcagcacca gcaaaccata gcgcaggccc aaagtcggca gcggaaaatg
     8521 gccatagacc accgtcaccc agtgcggaaa tagcgcgcgc acatcgtcag gcaaatatgc
     8581 cgccagcagc cagccgacaa aacccaccca caccgaccac aaaacggatt gcacggcccg
     8641 agtggtccag taggcggttt tatggcgcat ggtttcatca ttggcccaca gcaccgggct
     8701 gagcagcagc gccaccggcc cccacagcca ggcgctggct ttgaggttcc agcggtagag
     8761 caaggcgggg aggtaaatga tggtggcgag tgccgtaaaa aaaagaaata tgaaaatctt
     8821 gtcgccaaaa ttgccctcaa tcttttgccc aagccagcct tgcaaactaa acacaccgct
     8881 aatttctcct gctcccggca agagctccgg cagatgccgg gcatcaataa cacaaactgt
     8941 ttcgcgcc
//
LOCUS       DAOPYX010000112         8935 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_9282_length_8935_cov_8.245270, whole genome shotgun sequence.
ACCESSION   DAOPYX010000112 DAOPYX010000000
VERSION     DAOPYX010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 8935)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8935)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8935
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9282_length_8935_cov_8.245270"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..735)
                     /gene="recO"
                     /locus_tag="PKN55_07195"
     CDS             complement(<1..735)
                     /gene="recO"
                     /locus_tag="PKN55_07195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014086770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HNX71064.1"
                     /translation="MSQPGKVDHQPAFVLHSVPYKETSLIVELFTRDHGRVSVVARGA
                     RRPRAALRGLLLPFQPLKVGWFGKTELKTLAHADWQGALPQLGGVPLLAGLYLNELLL
                     RLTAREDPHSELFADYARAIRALGPRDGQGEPLSAQLRRFELRLLAELGYAPDLRCTQ
                     DGAPLAAECRYHCQLPAGVSAHADGEYSGAALLALAEGDFSTPAALRAARTLTRQWLD
                     HCLGEPPLATRALMAQLAELGARASPP"
     gene            complement(749..1726)
                     /gene="era"
                     /locus_tag="PKN55_07200"
     CDS             complement(749..1726)
                     /gene="era"
                     /locus_tag="PKN55_07200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003679342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HNX71065.1"
                     /translation="MQDHDQDHDAGDAGLSPEQIDAALAGLAPNVPEDYRCGFVAIVG
                     RPNVGKSTLMNHLIGQKISITSKKAQTTRHRVTGIQTTDKAQYIFVDTPGFQTRHRNA
                     LNEALNRSVKDSLSDVDCVLFVLEAGRLSDADREVIALLPAHRPVILVVNKADRLKDK
                     ADMANFLLKASAEFNFTEIVSVSAKHGHRLDTLLKVVEPHLPQSVPLYPEDMVTDKNE
                     RFLAAELVREKLFRYLGEELPYSMNVEIEKFEIEGNLRRIYAAVLVDKPGQKAILIGR
                     DGERMKKIATEARTDMEKLFDSKVFLEVWVKVKSGWADDVRFLKQFNLD"
     gene            complement(1731..2441)
                     /gene="rnc"
                     /locus_tag="PKN55_07205"
     CDS             complement(1731..2441)
                     /gene="rnc"
                     /locus_tag="PKN55_07205"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012696940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HNX71066.1"
                     /translation="MTHPLNLDRLSRRLGHAFRQPQLLRQALTHRSYSATNNERFEFI
                     GDSILNYTVARMLYDRFTQLSEGELSRLRANLVNQSTLADIARELSIGDCLFLGEGEL
                     KSGGHDRASILADALEALFAAVSFDADFNQAEAVVRQLYHSRITAIDPNKHAKDAKTR
                     LQEALQAKRYSLPRYTILDQTGEAHEQSFRVCCDLAEIGICTQGQGGSRRAAEQQAAE
                     RALEALDAQARHNAPRKN"
     assembly_gap    2528..2627
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2628..3559)
                     /gene="lepB"
                     /locus_tag="PKN55_07210"
     CDS             complement(<2628..3559)
                     /gene="lepB"
                     /locus_tag="PKN55_07210"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003742950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HNX71067.1"
                     /translation="MNWTVIFAVLTVLGPILIATSAKKQAGDKDQPMGVQAGYLALVL
                     GGFGLLAQWLSFSAVMLVFVLVTGVITAANRWLLAPRRDGGALEPHYVEYAKSFFPIM
                     LAVFMLRAFLVEPFQIPSSSMRPGLVVGDFILVNKFAYGVRTPIINNVLIPVGQVQHG
                     DVVVFNFPPDPKVNFIKRVIGLPGDTVSYRDKQLTVNGKALPLQEDGAYSYVETGKGA
                     SMVQTRRLKEQTPDGREYHVLHEDGKPSVFTPAVADFPGREHCQYDERGFTCKVPAGQ
                     YLMLGDNRDNSHDGRYWGFVEDKLLVGKAFMVWM"
     gene            complement(3605..5398)
                     /gene="lepA"
                     /locus_tag="PKN55_07215"
     CDS             complement(3605..5398)
                     /gene="lepA"
                     /locus_tag="PKN55_07215"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HNX71068.1"
                     /translation="MKHIRNFSIIAHIDHGKSTLADRFIQYCGGLSDREMDAQVLDSM
                     DIERERGITIKAQTAALRYKARDGQVYNLNLIDTPGHVDFSYEVSRSLSACEGALLVV
                     DASQGVEAQTVANCYTALDLGVEVVPVLNKIDLPAAEPERVCEEIEDIIGIEATDAVR
                     ASAKSGIGIEDILETVVQKIPAPEGNPDGPLKALIVDSWFDNYVGVVMLVRVVDGVLR
                     PKDKILFMSTQAQHLCEQVGVFTPKSESRTELRAGEVGFIIAGIKELKSAKVGDTLTL
                     VNNPASEALPGFKEAKSQVFAGLYPVESHDYDALRDALEKLQLNDAALNFEPEVSQAL
                     GFGFRCGFLGLLHLEIVQERLEREYDMDLITTAPTVVYEVVMKDGEQIEVENPSKLPD
                     LGKIQEIREPIITSTILVPQDYVGAVMTLCNQKRGVQRNMQYMGRQVMLTYDMPMNEV
                     VMDFFDKLKSTSRGYASLDYEFKEFQPSDLVKLDIMVNGEKVDALSLIVHRSTSVYRG
                     RELASKMRELIPRQMFDIAVQATIGSHIIARETIKALRKNVLAKCYGGDITRKKKLLE
                     KQKAGKKRMKQVGNVEIPQAAFLAILQVSDK"
     gene            complement(5489..5728)
                     /locus_tag="PKN55_07220"
     CDS             complement(5489..5728)
                     /locus_tag="PKN55_07220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008953847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HNX71069.1"
                     /translation="MHLTLYFREYCSLCHAMRDALRPYQQQHGFTLEVIDVDNDPALV
                     ERFDELVPVLMHGDTEICHYHLDAARLDAVLAEIG"
     gene            complement(5801..7216)
                     /locus_tag="PKN55_07225"
     CDS             complement(5801..7216)
                     /locus_tag="PKN55_07225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011135609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HNX71070.1"
                     /translation="MSTRTLSRRLVSLTLTVGMLLSAVAHAARDLPDFTRLVESQGRT
                     VVNISTTQTVKTVAGPFGDDAANDLFSEFFRRFGVPGGGQPREQQQSSLGSGFLISAD
                     GYIMTNAHVVAKADEITVSTTDKREYKAKLVGADTRTDVAVLKIDGHSLPTVSLGNPS
                     LLKPGEWVVAIGSPFGFDNSVTAGIVSAKGRQLPGDAYVPFIQTDVAVNPGNSGGPLF
                     NLDGQVVGINSQIYSRSGGFMGISFAIPIDVAMQVADQLKAHGRVSRAVIGVSVQELS
                     RDLAASFGLSRPSGALINSVQPGGPADKAGLKSGDIVLQVGGKPVENASDMARLISSA
                     RPGQPLALEIWRNKASRTLTVTPNEMSEQASERQGRNRQPNAAPALTGLNMSLSELSP
                     AQLRALRISYGLMVQRVQGLAARAGVQPGDVIVGVNNSTLTSAEQFREQVAAAKAGDT
                     LALRLIRDGNTLFVAVPVAEK"
     gene            complement(7177..7671)
                     /locus_tag="PKN55_07230"
     CDS             complement(7177..7671)
                     /locus_tag="PKN55_07230"
                     /inference="COORDINATES: protein motif:HMM:NF016160.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR reducing system RseC family protein"
                     /protein_id="HNX71071.1"
                     /translation="MIEAEARVLRCDGEQAWVELRPHTPCGHCDPQRGCRSLSLARAF
                     HTGQSAFAVANPLSAQAGDWVVVAARADSVRHSALLLYGAPLAALLAGALLGAPLGEG
                     WSMALSGLGLAASLLALAWHARRAAGLDAHRPVITRLLSQPSSTARNVSSCQHARSPG
                     DWSA"
     gene            complement(7668..8633)
                     /locus_tag="PKN55_07235"
     CDS             complement(7668..8633)
                     /locus_tag="PKN55_07235"
                     /inference="COORDINATES: protein motif:HMM:NF015825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucB/RseB C-terminal domain-containing protein"
                     /protein_id="HNX71072.1"
                     /translation="MHRQVLAWTAWLAMAPAWAASVLPDGEAQQLLKRYVGAVRQQSY
                     AGVYLHQVGGSVESYRLAHSHEGGIWREKRESLDGVRWELIRQGNELTAYGADTHALA
                     EAKLSTQRMFPELLPDNPAELTTVYSVRQLEHDRVAGYDCNWTVLEPRDNARYAYHFC
                     IEDKTSLPLRISVINGRREVIEQFTFSQLSIGPVRDKNAFRPTFDQQHPIMPAGGNLE
                     DGRADRPEIKVTPAGFRLLRDVRRTMSGRSQSVRHLVYSDGLASFSVFIEPGVAANRS
                     RLAGYFARGALRAYSRPVGDTLVTVVGDLPDATIQSIANSVQLKP"
     gene            complement(8633..>8935)
                     /locus_tag="PKN55_07240"
     CDS             complement(8633..>8935)
                     /locus_tag="PKN55_07240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX71073.1"
                     /translation="AALPQPASRTRRASRWVSLALAASAALVAVIVGVQSDNLSEDAQ
                     LASAVLSTPAPEAVQTLAMAPAAPEAPANNPYMQAHQESVSNPYLMTASYSSEGRR"
BASE COUNT         1485 a         2729 c         2931 g         1690 t
ORIGIN      
        1 cggcgggctg gcgcgcgcgc ccagctcggc cagttgcgcc atcagcgcgc gggtggccag
       61 cggcggctcg cccaggcagt ggtccagcca ctggcgggtg agcgtgcgcg cggcgcgcaa
      121 ggcggccggg gtggaaaaat ccccctcggc caaggccagc agcgccgcgc cggaatactc
      181 gccgtctgcg tgggcgctga caccggccgg caactggcaa tgatagcgac attcggcggc
      241 cagtggcgcg ccgtcctggg tgcagcgcag gtcgggcgcg tagcccagct cggccagcag
      301 gcgcagctca aagcggcgca gctgggcgct gagcggctcg ccctggccgt cgcgcgggcc
      361 cagcgcgcgg atggcgcggg cgtagtcggc aaacagttcg ctgtgcgggt cttcgcgggc
      421 ggtcagccgc agcagcagct cattcagata tagcccggcc agcaggggca cgccgcccag
      481 ctgcggcagg gcgccttgcc agtcggcgtg ggccagggtt ttcagctcgg ttttgccaaa
      541 ccagcctact tttaagggct gaaacggcag cagcaggccg cgcagcgcgg cgcgcgggcg
      601 ccgggcgccg cgcgccacca ccgacacccg gccgtggtcg cgggtgaaca gttcgacaat
      661 caggctggtt tctttgtacg gcacgctgtg cagcacaaag gccggctggt ggtccacctt
      721 gccaggctgg gacatggcgg tttggcgctc agtccaggtt aaattgcttc aggaagcgca
      781 cgtcgtcggc ccagccgctc ttgaccttga cccagacttc cagaaacacc ttgctgtcga
      841 acagtttttc catatcggtg cgcgcttcgg tggcgatttt tttcatgcgc tcgccgtcgc
      901 ggccaatcag gatggctttc tggccgggct tgtccaccag cacggcggcg tagatgcgcc
      961 gcagattgcc ttcgatctcg aatttttcga tttccacgtt catcgaatag ggcagttctt
     1021 cgcccaggta gcggaacagt ttttcccgca ccagctcggc ggccagaaag cgctcgttct
     1081 tgtcggtgac catgtcttcc gggtagagcg gcacgctttg cggcaggtgc ggctccacca
     1141 ctttcagcag ggtgtccagc cggtggccgt gcttggcgct gaccgagacg atttcggtaa
     1201 agttgaattc ggcgctggcc ttgagcagaa aattggccat gtcggctttg tctttcagcc
     1261 ggtcggcctt gttcaccacc agaatcaccg ggcggtgcgc cggcaagagg gcaatcacct
     1321 cgcggtcggc gtcagacagg cggccggctt ccagcacaaa cagcacgcag tccacatcgg
     1381 acagcgaatc cttgacgctg cggttcagcg cctcgttcag cgcattgcga tggcgggttt
     1441 gaaagcccgg cgtatcgaca aaaatatact gcgccttgtc ggtggtctga atgccggtga
     1501 cgcggtggcg ggtggtctgg gcttttttcg aggtgatgct gatcttctgg ccgatcaggt
     1561 ggttcatcag cgtggatttg cccacattcg gccggccgac aatggcgaca aagccgcaac
     1621 ggtagtcttc gggcacattg ggcgccaggc cggccagcgc ggcgtcgatt tgttcggggc
     1681 tcaggccggc gtcgccagcg tcgtggtctt ggtcgtggtc ttgcatggtg tcagttcttt
     1741 cgcggggcgt tgtggcgggc ctgggcgtcc agcgcctcca gcgcgcgttc tgcggcttgt
     1801 tgctcggcgg cgcggcggct gccgccctgg ccctgggtgc aaatgccgat ttcggccagg
     1861 tcgcaacaga cgcggaagga ttgttcgtgg gcctcgccgg tctggtcgag gatggtgtag
     1921 cgcggcaggc tgtaacgctt ggcttgcagg gcttcctgca ggcgggtttt ggcgtccttg
     1981 gcgtgcttgt tcgggtcgat ggcggtgatc cgcgaatggt agagctggcg caccacggct
     2041 tcggcctggt tgaagtcggc gtcaaagctg acggcggcga acagcgcttc cagcgcatcg
     2101 gccagaatcg aggcgcggtc gtggccgccg cttttcagct cgccttcgcc cagaaacagg
     2161 cagtcgccga tgctcagctc gcgggcgata tcggccagcg tgctctggtt gaccaggttg
     2221 gcgcgcagcc gcgacagttc gccttcgctg agctgggtga agcggtcgta gagcatgcgc
     2281 gccacggtgt agttcaggat gctgtcgcca atgaattcaa agcgttcgtt attggtggcg
     2341 ctgtagctgc ggtgggtcag cgcctggcgc agcagttgcg gttggcgaaa cgcatggccc
     2401 agccggcgcg acagccggtc aaggttgagg ggatgagtca ctgcctgagt ctgccttagc
     2461 ggtgcgagtc aaaaaaatgg aaaacgccgg gggctgcgcc gccggcggca agaatgggta
     2521 ttgtagcnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnttc atccacacca
     2641 taaacgcctt gcccaccagc agtttgtctt ccacaaagcc ccagtagcgg ccgtcgtggc
     2701 tgttgtcgcg gttgtcgccc agcatcaggt actggccagc ggggactttg caggtaaagc
     2761 cgcgttcgtc gtactggcag tgctcgcggc cggggaagtc ggccacggcg ggggtgaaca
     2821 cgctgggttt gccgtcttcg tgcagcacat ggtattcccg gccgtccggg gtttgctctt
     2881 tcagccggcg ggtttgcacc atgctggcgc ctttgccggt ttccacatag ctgtaggcgc
     2941 cgtcttcttg cagcggcagg gctttgccgt tgacggtgag ttgcttgtcg cgataactca
     3001 ccgtgtcgcc gggcaggccg atgacgcgct tgatgaagtt cactttcggg tcaggcggaa
     3061 aattgaacac caccacgtcg ccgtgttgca cttggccgac cgggatcagc acattgttga
     3121 tgattggcgt gcgcacgccg taggcgaatt tgttgaccag gatgaaatcc cccaccacca
     3181 ggccggggcg catggacgag gacgggattt gaaacggctc gaccaaaaag gcgcgcagca
     3241 tgaacacggc cagcatgatg gggaaaaagc tcttggcgta ttccacataa tgcggctcca
     3301 gcgcgccgcc gtcgcggcgc ggggccagca gccagcggtt ggcggcggtg atcacgccgg
     3361 tcaccagcac gaacaccagc atcaccgccg agaagctcag ccactgggcc aacaggccaa
     3421 agccgcccag caccagggcc aggtagccgg cttgcacgcc catgggctgg tctttgtcgc
     3481 cggcttgttt tttcgccgag gtggcgatca ggatcgggcc gagcacggtg agcacggcaa
     3541 aaatcacggt ccagttcatc agtgtcctta cggttgcggc gcgggcgggc gtgagcccgc
     3601 gcggttattt gtcgctgacc tgcaagatgg ccaggaaggc ggcttgcggg atttccacat
     3661 tgcccacctg cttcatgcgc ttcttgccgg ctttttgttt ttccagcagc tttttcttac
     3721 gggtgatgtc gccgccgtag cacttggcca gcacgttctt gcgcagggcc ttgatggttt
     3781 cccgggcaat gatgtggctg ccgatggtgg cttgcacggc gatgtcgaac atctggcgcg
     3841 gaatcagctc gcgcattttc gacgccagtt cacggccacg gtacacgctg gtggagcggt
     3901 gcacgatcag gctcagggcg tccaccttct cgccattgac catgatgtcg agtttgacca
     3961 gatcagacgg ctggaattcc ttgaactcgt aatccagcga ggcgtagcca cggctggtgg
     4021 atttgagctt gtcaaagaag tccatcacca cttcgttcat cggcatgtcg taggtcagca
     4081 tcacctgacg gcccatgtac tgcatattgc gctgcacgcc gcgcttttgg ttgcacagcg
     4141 tcatcaccgc gcccacatag tcctgcggca ccaggatggt ggaggtgatg atgggttcgc
     4201 ggatttcctg aatcttgccc aggtccggca gcttggacgg gttttccacc tcgatctgct
     4261 cgccgtcctt catcaccact tcgtagacca cggtcggcgc ggtggtgatg aggtccatgt
     4321 catattcgcg ctccagccgc tcctggacga tttccaggtg caacaggccc aaaaagccgc
     4381 agcggaaacc aaagcccagc gcttgcgaga cttctggctc aaagttcagc gcggcgtcgt
     4441 tcagctggag tttttccagc gcgtcgcgca gcgcgtcgta gtcgtggctt tccaccgggt
     4501 agaggccggc aaacacctgc gacttggctt ccttgaaacc aggcagcgcc tcgctggccg
     4561 ggttgttcac cagggtgagc gtatcgccca ccttggcgga tttcagctct ttgatgccgg
     4621 cgatgataaa gcccacctca ccggcgcgca gctcggtgcg cgattcggat ttcggggtga
     4681 acacgcccac ttgctcgcac agatgctgag cctgggtgga catgaacaaa atcttgtcct
     4741 tggggcgcag cacgccatcc accacgcgca ccagcatcac cacgccgacg tagttgtcaa
     4801 accacgagtc aacaatcagc gccttcagcg ggccatccgg attgccttcc ggcgccggga
     4861 ttttctgcac cacggtttct aggatgtctt cgatgccgat gccgctcttg gccgaggcgc
     4921 gcacggcgtc ggtggcttcg atgccgatga tgtcttcgat ttcttcgcac acgcgctcgg
     4981 gttcggcggc cggcaagtcg atcttgttca gcaccggcac cacttccacg cccagatcca
     5041 gcgcggtgta gcagttggcc acggtttgcg cctcgacgcc ttgcgaggcg tccaccacca
     5101 gcagcgcgcc ttcgcaggcg gacagcgagc ggctgacttc gtagctgaag tccacgtggc
     5161 cgggggtgtc gatcaggttg aggttgtaca cctggccatc gcgcgccttg tagcgcagcg
     5221 cagcagtctg cgccttgatg gtgatgccgc gctcgcgctc gatgtccatc gagtcaagca
     5281 cctgggcatc catctcgcgg tcggacaagc cgccgcaata ctggatgaat cggtcggcca
     5341 gggtcgattt accgtggtcg atgtgggcga taatggaaaa gttgcggata tgcttcatgg
     5401 cgggtcggga tagaaaggcc gcgccggccc gtgggctgga cgcgtccccg gcgcgctggg
     5461 cgccatggat ggttgaatgg cggcgattct agccgatttc ggccagcact gcatccagtc
     5521 gcgcggcatc cagatggtaa tggcagattt ctgtgtcgcc atgcatcaac accggcacca
     5581 gttcgtcaaa gcgttcgacc agcgccgggt cgttgtccac gtcgatcact tccagggtga
     5641 agccgtgttg ctgctgatac ggacgcaggg cgtcgcgcat ggcgtggcac aggctgcaat
     5701 attcacgaaa gtacaaagtc aggtgcatgg cgggctcggc aatcagggaa aaacaggccc
     5761 gccatgggcg ggccatggcg ggcggtgagg cgcaatgggt ttatttttcg gccaccggca
     5821 cggcaacaaa cagcgtgttg ccgtcacgaa tcagacgcag cgccagggtg tcgccggctt
     5881 tggcggcggc cacctgctcg cggaactgct cggcgctggt cagcgtgctg ttgttgacgc
     5941 caacaatcac atcgccaggt tgcacacccg cgcgggcggc caggccttgc acgcgctgca
     6001 ccatcaggcc gtaagagatg cgcagggcgc gcagttgcgc cggactcagc tcgctcaagc
     6061 tcatgttcag cccggtcagc gcgggtgcgg cattcggctg gcggttgcgc ccttggcgct
     6121 cgctggcttg ctcgctcatt tcattcgggg tgaccgtcag cgtgcggctg gccttgttgc
     6181 gccagatttc cagcgccagc ggctgcccag ggcgtgcgct gctgatcagc cgcgccatat
     6241 cgctggcgtt ttctaccggt ttgccgccca cttgcagcac gatgtcgcca gatttcaggc
     6301 cggccttgtc ggccgggccg ccgggctgca cgctgttgat cagcgcgccg ctggggcggc
     6361 tcaggccaaa cgaggcggcc agatcgcggc tgagctcttg caccgagacg ccaatcaccg
     6421 cgcggctgac ccggccatgc gccttgagct ggtcggccac ctgcatggcg acatcaatcg
     6481 ggatggcgaa agaaatgccc ataaagccgc cggagcggct gtaaatctgc gagttgatgc
     6541 ccaccacctg gccatccaga ttgaacagcg gcccgccgga attccccgga ttcaccgcca
     6601 catcggtctg gataaacggt acataggcgt cgcccggcag ctggcggccc ttggccgaga
     6661 caatgccggc agtcactgaa ttgtcaaaac caaatggcga gccaatcgcc accacccatt
     6721 cgcctggctt gagcagggac gggttgccca ggctgacggt gggcagactg tggccgtcga
     6781 ttttcagcac ggccacgtcg gtgcgggtgt cggcgccgac caattttgcc ttgtattcgc
     6841 gcttgtcggt ggtgctgacg gtaatttcat cggccttggc caccacatgg gcgttggtca
     6901 tgatgtagcc gtcggcagaa atcaaaaagc ccgagcccag cgagctttgc tgttgctcgc
     6961 gcggctggcc gccgccgggc acgccaaagc ggcggaagaa ttccgaaaac aaatcattgg
     7021 cggcgtcatc gccaaacggg ccggccacgg tcttgaccgt ttgcgtggtg ctgatattga
     7081 ccacggtgcg gccctggctt tccaccaggc gggtaaagtc gggtaaatcg cgggcggcgt
     7141 gggccacggc gctgagcaac atgcccacag tcagcgtcag gctgaccagt cgccgggaga
     7201 gcgtgcgtgt tgacatgagg agacgttccg agcagttgag gaaggttgag aaagcaaacg
     7261 ggtaatcacc ggccgatggg cgtccaggcc ggcggcgcgg cgggcgtgcc aggccagcgc
     7321 cagcaggctg gcggccaggc ccaggcccga cagcgccatt gaccagcctt cgcccagcgg
     7381 cgcgcccagc aaggcgccgg ccagcagggc ggccagcggc gcgccataca gcaacagcgc
     7441 gctgtggcgc acgctgtcgg cgcgggcggc taccaccacc cagtcgcccg cttgcgcgga
     7501 caaagggttg gccacggcaa acgccgattg gccggtgtga aacgcgcgcg ccaggctcag
     7561 cgagcggcag ccgcgctgtg ggtcgcagtg cccgcacggg gtgtgcgggc gcagctccac
     7621 ccaggcttgc tcaccgtcgc agcgcagcac gcgggcttca gcttcgatca tggcttgagt
     7681 tgcaccgagt tggcaatcga ctgaatggtg gcgtcaggca gatcgcccac caccgtcacc
     7741 agggtgtcgc ccaccggccg gctgtaggcg cgcagcgcgc cacgggcaaa atacccggcc
     7801 agacgtgaac ggttggcggc cacccccggc tcgatgaaca ccgagaaact ggccaggccg
     7861 tcgctataca ccaagtggcg caccgactgg ctgcgcccgc tcatggtgcg gcgcacatcg
     7921 cgcaacaggc gaaagcccgc tggtgtgact ttgatctccg ggcggtcggc gcggccgtcc
     7981 tccagattgc cgccggccgg catgatcgga tgctgctggt cgaaggtggg gcgaaacgcg
     8041 tttttatcgc gcaccgggcc gatgctgagc tggctgaagg tgaattgctc gatcacttcg
     8101 cgccggccgt tgattacgct gatgcgtagc ggcaggctgg ttttgtcttc gatgcaaaag
     8161 tgatacgcat agcgggcatt gtcacgcggc tccagaactg tccagttgca gtcgtagccg
     8221 gccacgcgat cgtgttccag ttggcgtacg ctgtacaccg tggtcagctc ggcaggatta
     8281 tccggcagca gttccggaaa catgcgctgg gtggagagct tggcctcggc cagcgcatgg
     8341 gtatcggcgc cgtaggcagt cagctcgttg ccctggcgga tcaactccca gcgcacaccg
     8401 tccagtgatt cgcgcttttc ccgccagata ccgccttcgt ggctgtgcgc cagccggtag
     8461 gactccaccg agccgcccac ttgatgcaga tatacgcccg catagctttg ctggcgcact
     8521 gcgccgacat agcgcttgag cagttgctga gcctcgccgt cgggcaatac cgaagccgcc
     8581 caggccggcg ccatcgccag ccaggccgtc catgccaaaa cctgtctgtg catcaacgac
     8641 ggccctcact gctgtacgaa gccgtcatca gatacggatt agacaccgac tcttgatgcg
     8701 cctgcatata cgggttattg gccggcgctt ccggggcggc cggcgccatg gccagcgttt
     8761 gcacggcctc gggcgccggc gtgctgagca cggcactggc cagttgtgcg tcttcgctga
     8821 ggttgtccga ctgcacgccg acaatcaccg ccaccagcgc agcactggcc gccagggcca
     8881 gactgaccca gcgtgaagcg cggcgggtgc ggctggccgg ttgcggcagg gcggc
//
LOCUS       DAOPYX010000113         8769 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_9509_length_8769_cov_9.577576, whole genome shotgun sequence.
ACCESSION   DAOPYX010000113 DAOPYX010000000
VERSION     DAOPYX010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 8769)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8769)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8769
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9509_length_8769_cov_9.577576"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..682
                     /locus_tag="PKN55_07245"
     CDS             <1..682
                     /locus_tag="PKN55_07245"
                     /inference="COORDINATES: protein motif:HMM:NF003975.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016706 - 2-oxoglutarate-dependent
                     dioxygenase activity [Evidence IEA];
                     GO_function: GO:0031418 - L-ascorbic acid binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Fe2+-dependent dioxygenase"
                     /protein_id="HNX71074.1"
                     /translation="MLLHIPNVLTHDELRQARQLLAEAPWEDGLATAGAQSALVKHNL
                     QLAQTSPQAQALGSLVLAALNRNALFFSAALPNRIFPPMFNCYQGGMTFGNHVDNAVR
                     NDPFSGQWLRTDVSCTLFFTDPDEYQGGELIVEDTYGSHAVKLPAGDMILYPSTSVHR
                     VEPVTAGARLASFFWTQSMIRDDNRRAMLFDMDSAISSLRQRHGDSEETVNLTSVYHN
                     LLRQWSEV"
     gene            complement(752..2149)
                     /locus_tag="PKN55_07250"
     CDS             complement(752..2149)
                     /locus_tag="PKN55_07250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014086134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HNX71075.1"
                     /translation="MQDSQQNLHRGLEERHINLMALGAAIGVGLFLGSAKAIQMAGPA
                     IMVSYALGGFAIFLIMRALGEMAIHDPVAGSFSRYAQNYLGPLAGYLTGWNYWFLWIV
                     TCMAEITAVGVYMGVWFPGVSSWVWALSALVIMGSVNFIAVKAYGEFEFWFALIKIVT
                     IVLMIISSLGMILFGFGNGGVATGIAHLWQHGGFMPNGVQGILMSMQMVMFAYLGVEM
                     IGLTAGEAKNPRKTLARAVDSVFWRILIFYVGALFVVMSIYPWNEIGAQGSPFVMTFE
                     KMGIPAAAGLINFVVLTAALSSCNNGIFSTGRMLYNLAQQEQALPMFAKVGKNGVPNA
                     AVLISVVMLLGGVLLNYLAPEEVFTWLTAISTFGAIWTWVIILLSQLKFRQTLSRSEV
                     AALACRMPFFPYGSYIALAFLVLVVGLMAYFPDTRIALLVGPGWLLLLIVLYYALGYH
                     RKPVSLPTAPAASQA"
     gene            complement(2525..4591)
                     /gene="ppk1"
                     /locus_tag="PKN55_07255"
     CDS             complement(2525..4591)
                     /gene="ppk1"
                     /locus_tag="PKN55_07255"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007341556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HNX71076.1"
                     /translation="MTTPPLPLLNRELGLIEFNRRVLSQAEDTGVPLLERLKFLCIVS
                     SNLDEFFEVRVASLKEAQRLSPGQALPDGLLPSQALAKVSQAAHGLVERQYWLMREVL
                     FPALAEEGIHFVRRAEWTAEMHEWIHQYFLREVMPVLTPIGLDPSHPFPRLLNKSLNF
                     AIELEGRDAFGRSSGIAIVQAPRILPRVIKLPEHIAGKPNCFIFLSSMLHAHIGELFS
                     GMAIKGFYQFRLTRDSELTVEEDELKNLRTALQGELRHRQYGDAVRLEIADNCPEHME
                     EFMLGQFGLEKADVYRVEGPVNLVRLMQVPDLVDRPDLKYRPFLPGLPLPLAKKNDLL
                     AAIRKGDVLLHHPYQSFQPVIDLLNIAATDPQVVAIKMTVYRTGTESALMDSLLAAAR
                     AGKQVTVVVELMARFDEEANIGWASRLEDAGAHVVYGVYGYKIHAKMLMVVRREDGQL
                     RRYVHLGTGNYHPRTARMYTDFGLMTCNEEIASDVNDIFVQITGLGRVGELKHLLQSP
                     FSLHSGMLAAISREAEHARAGRSAAIIAKMNSLLEPQIIAALYEASQAGVKIRLIIRG
                     VCALRPGVPGLSDNIEVRSVVGRFLEHPRVFYFYNDRQEDLYIASADWMGRNFFRRIE
                     TCVPIRDTKLKRRVIKEGLRPYLFDNVNAWEMQGDGSYKRKTQRGKPRSAQAILLEEL
                     SSPTVR"
     gene            complement(4671..4943)
                     /locus_tag="PKN55_07260"
     CDS             complement(4671..4943)
                     /locus_tag="PKN55_07260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018746185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HNX71077.1"
                     /translation="MTRMVNCIKLGREAEGLDVPPMPGELGKKLYDSVSKEAWQAWLK
                     LQTMLINENRLNLADPRARQYLTQQMQNHFFGSGSEMPTGYVPPQA"
     gene            complement(5125..5367)
                     /locus_tag="PKN55_07265"
     CDS             complement(5125..5367)
                     /locus_tag="PKN55_07265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003746468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HNX71078.1"
                     /translation="MTAQTFSAEEKTALLALKGVGPTVVLRLEQLGIHSFAQLATEDA
                     DVLAQRVAALVGGSCWRNSPQARAALAACIAQAKTA"
     gene            complement(5453..7729)
                     /gene="metE"
                     /locus_tag="PKN55_07270"
     CDS             complement(5453..7729)
                     /gene="metE"
                     /locus_tag="PKN55_07270"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008953282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HNX71079.1"
                     /translation="MIKIHNLGFPRIGAQRELKFALERHWRGEDSLEALEHTARTLRQ
                     RHWLTQKAAGLDWIPVGDFACYDHVLSTLFLLGALPGRFAIDPAQATLADQFTLARGN
                     ASQPAMEMTKWFDTNYHYLAPEFDRHTTFAAQPRWLLEPLAEARALGLAAKPVLLGPL
                     SLLWLGKSKDGVDRLSLLPALLSAYGELLSALSIHGVDWVQIDEPILGLDLPADWLAA
                     YQPAYAQLAAQAPRLLLASYFASVLPQRDLLKRLPVAGVHLDVVRAPEQLAGFVADWP
                     RERVLSLGVIDGRNIWRADLNALLTQLAPLAAQWGEQLWLAPSCSLLHVPVDLAQETE
                     LDAEVKTWLAFAVQKLNELKLLARGLNHGADSIANELAACRHAHNSRRASPRVVNPAV
                     RQRLADIRPGDDQRRSAYPQRARAQHDWLKLPLLPTTTIGSFPQTADIRAARAAWRAG
                     KLDAASYDAAMRAEITLAVRKQEALGLDVLVHGEAERNDMVEYFGEQLDGFVFTRHGW
                     VQSYGSRCVKPPVLFGDVSRPQPMTVAWSVFAQSLTERPMKGMLTGPVTILQWSFVRD
                     DLARAEVCRQIALAIRDEVLDLENAGIRVIQIDEPAFREGLPLKRADWDAYLDWAGQS
                     FRLSAAGVDDATQIHTHMCYSEFNDILPAIAALDADVITIETSRSDMELLDAFAEFAY
                     PNEIGPGVYDIHSPRIPDSAEMVKLLNKALAVIPARRLWVNPDCGLKTRNWAETEAAL
                     ANMLTATRQLRAQLAATA"
     gene            7847..>8769
                     /locus_tag="PKN55_07275"
     CDS             7847..>8769
                     /locus_tag="PKN55_07275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008953281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HNX71080.1"
                     /translation="MATSLLELRHLRTLLALAECGNVSAAARRLNLTQSALSHQLKTL
                     ESHYGCALVDRRRQPLALTAAGERLHTLARSVLAQVQQAERELARIAQGEAGQLRIAV
                     ECHTCFDWLMPAMDDFRGHWPEVELDIVSGFHADPVGLLLSRRADLAVVSAPEDEAAV
                     RYHPLFGYEMVAVLARDHPLAGKPWLSAADFARETLITYPVPDDKLDLVWQVLRPAGI
                     NPPRRTTELTVAMVQLVASRRGVAALPFWAVKPYLERGYVQALPIGEHGLRSELYAAT
                     RADDAGLAYLEDFLVTVRELSFATLAGIRPLA"
BASE COUNT         1619 a         2721 c         2848 g         1581 t
ORIGIN      
        1 catgctgctg cacattccca acgtcctcac ccacgacgaa ctgcgccagg cgcgccaact
       61 gctggccgaa gcgccgtggg aagacggcct ggctaccgcc ggcgcccaat ccgctctggt
      121 caaacacaat ctgcaactgg cgcaaacctc gccacaggcg caagcgctgg gcagcctggt
      181 gctggcggcg ctgaaccgca atgcgctgtt tttctcggca gcgctgccca accggatttt
      241 tccgccgatg ttcaactgct atcaaggcgg catgaccttt ggcaatcacg tggacaatgc
      301 cgtgcgcaac gacccgttca gcggccaatg gctgcgcact gacgtatctt gtacgctgtt
      361 tttcaccgac ccggacgagt atcaaggcgg cgagctgatc gtggaagaca cctacggcag
      421 ccacgccgtc aagctgccgg ccggcgacat gattttgtat ccgtccacca gcgtgcaccg
      481 cgtggagcca gtcacggccg gcgcgcggct ggcgtcgttt ttctggacgc aaagcatgat
      541 ccgcgatgac aaccgccgcg ccatgctgtt tgatatggac agcgcgattt ccagtctgcg
      601 ccagcgccat ggcgacagcg aagagacagt gaacctgacg tcggtgtatc acaatctgct
      661 gcggcaatgg agcgaggtgt aaaaccgcgc ctgccgatac gcgtcgataa aaaagcccgc
      721 atttgcgggc tttttcctga gcggggtgat tttaggcttg cgatgccgct ggcgccgtcg
      781 gcaagctcac cggcttgcga tggtagccca gtgcgtaata cagcacgatc agcagcagca
      841 accaacccgg ccccaccagc aaggcaatgc gggtgtccgg aaaatacgcc atcagaccga
      901 ccaccagcac caggaaggcc agggcaatat acgagccata cggaaagaac ggcatccggc
      961 aggccagcgc ggccacttcg ctgcgggaca atgtctggcg gaacttcagc tgcgacagca
     1021 ggatgatcac ccaggtccag atggcgccaa aggtggaaat cgccgtcagc caggtaaata
     1081 cctcttctgg ggccaggtag ttgagcagca cgccgcccag cagcatcacc actgaaatca
     1141 gcaccgcagc attgggcacg ccgttcttgc ccaccttggc aaacatgggc agcgcctgct
     1201 cttgctgcgc caggttgtac agcatgcggc cggtgctgaa aataccgtta ttgcacgacg
     1261 acagcgcagc agtcagcacc acaaagttga tcagcccggc ggcggccgga atgcccattt
     1321 tttcgaaggt catcacgaag gggctgccct gcgcgccgat ttcattccac gggtagatcg
     1381 acatcaccac aaacagtgcg ccgacataaa aaatcaggat gcgccagaac accgaatcca
     1441 ccgcgcgcgc cagcgtcttg cgcgggtttt ttgcttcgcc agcggtcagg ccgatcatct
     1501 ccacgcccag ataggcaaac atcaccattt gcatcgacat caaaatgccc tgcacgccat
     1561 tgggcataaa gccgccgtgc tgccacaaat gggcgatgcc ggtggccacg ccgccattgc
     1621 caaagccaaa cagaatcatg cccaggctgg aaataatcat cagcacaatg gtgacgatct
     1681 tgatcagggc aaaccagaat tcaaattccc cgtaggcctt gacggcaatg aaattgaccg
     1741 agcccatgat caccagtgcc gacagcgccc agacccagga cgacacgccc ggaaaccaca
     1801 cgcccatata cacgcccacg gcggtgattt ccgccatgca ggtgacaatc cacaagaacc
     1861 agtaattcca gccggtcaga tagccagcga gtgggcccag atagttttgg gcatagcggc
     1921 tgaacgagcc ggccaccgga tcgtggatgg ccatttcgcc cagtgcgcgc atgatcaaga
     1981 aaatggcaaa gccgcccagc gcatacgaaa ccatgatggc aggcccggcc atctggatgg
     2041 ccttggccga gcccagaaac aggcccacgc cgatggcggc gcccagcgcc atcaggttga
     2101 tatgcctctc ctcaagaccg cgatgcagat tttgttgtga atcttgcacg gggtgtttcc
     2161 tcctgtattg tggtggcgtc cgggcgggat aagcctggcg ccgttttttt gtggcagcag
     2221 atacgctgaa tcaggctgcc cgccgggcct gggtgcggcc gggcaggcgt gtttcgcgca
     2281 ggggcttccc ttacggaagc ggctagtata agaatttgcc ccgtgggttt ttcaccatac
     2341 ttcagccaga aatcgcagga attttcagtt tttcactgct tctggcggga atagcacaag
     2401 ggcatggtgt gggcgtggcg gcaatggcgc agagaggcgg gcgtatcaac tggtgacgcc
     2461 agcatgcaaa agcccgcacg gtgtgccgtg cgggcttggg cggggtaaat caacgcaaag
     2521 ggggtcagcg tactgtcggg ctgctgagtt cttccagcaa aattgcctga gcgctgcgcg
     2581 gcttgccgcg ttgcgtcttg cgcttgtagc tgccgtcacc ctgcatttcc caggcgttga
     2641 cgttgtcgaa cagatacggg cgcaggcctt ctttgatcac ccggcgcttg agcttggtgt
     2701 cgcggatcgg cacgcaggtt tcgatgcggc ggaagaagtt gcggcccatc cagtcagcgc
     2761 tggcgatgta aaggtcttcc tggcggtcgt tatagaagta gaacacccgt ggatgctcca
     2821 gaaaacggcc caccaccgag cgcacttcga tattgtccga cagccccggc acgccggggc
     2881 gcaaggcgca tacgccacgg atgatcaggc ggattttcac cccggcttgc gaggcctcgt
     2941 acagcgcggc gatgatttgc ggctccagca gcgagttcat cttggcgatg atcgccgccg
     3001 agcggccggc gcgggcgtgt tcggcttcgc ggctgattgc cgccagcatg ccgctgtgca
     3061 gcgagaacgg cgattgcagc aggtgcttga gctcgcccac ccggcccagg ccggtgatct
     3121 gcacaaagat gtcgttgacg tcgctggcga tttcctcgtt gcaggtcatc aggccaaagt
     3181 cggtgtacat gcgcgcggtg cgcgggtggt agttgccggt gcccagatgc acatagcggc
     3241 gcagctggcc gtcttcgcgg cgcaccacca tcagcatctt ggcgtgaatc ttgtagccgt
     3301 acacgccgta caccacatgc gcgcccgcgt cttccaggcg cgaggcccag ccgatattgg
     3361 cttcttcgtc aaagcgcgcc atcagctcga ccaccacggt gacttgcttg ccggcgcgcg
     3421 ccgccgccag cagcgaatcc atcagggcgg attcggtgcc ggtgcggtac accgtcatct
     3481 tgatcgccac cacttgcggg tcggtggcgg cgatgttcag caggtcaatc accggctgga
     3541 atgactgata cgggtgatgc agcagcacat cacccttgcg gatggcggcc agcagatcat
     3601 tctttttggc cagtggcagc ggcaggccgg gcaggaaggg gcggtattta aggtcggggc
     3661 ggtccaccag gtcgggcact tgcatcagcc gcaccaggtt gaccgggccc tccacccggt
     3721 agacgtcggc tttttccaga ccaaactggc cgagcatgaa ctcttccatg tgctcggggc
     3781 agttgtcggc gatttccagc cgcaccgcat cgccgtactg gcgatggcgc agctcgcctt
     3841 gcagggcggt gcgcaggttt ttcagctcgt cttcttcgac ggtcagctcg ctgtcgcggg
     3901 tcaggcggaa ctggtaaaag cccttgatcg ccatgccgga gaacagctcg ccgatatggg
     3961 cgtgcagcat cgacgacagg aagataaagc agttgggctt gccggcaatg tgttcgggca
     4021 gcttgatgac gcgcggcaga atgcgcggcg cctgcacgat ggcaatgcca gagctgcggc
     4081 caaaggcatc gcggccttcc agctcgatgg cgaagttcag gcttttgttc agcaaacgcg
     4141 ggaaggggtg ggatggatcc aggccgatgg gggtgagcac cggcatcact tcgcgcagga
     4201 agtattgatg aatccattcg tgcatctcgg cggtccactc ggcgcggcgg acaaagtgga
     4261 tgccttcttc ggccagcgcc gggaacagca cttcgcgcat cagccagtat tggcgctcca
     4321 ccagcccatg cgcggcctgc gagaccttgg ccagcgcctg gctgggcagc aggccgtcgg
     4381 gcagcgcctg gccgggcgac agccgttgcg cttctttgag cgaggcgacg cggacttcga
     4441 agaactcgtc gaggttggac gagacaatgc agagaaactt gagccgctcc agcagcggca
     4501 cgccggtgtc ttcggcttgc gacagcacgc ggcggttgaa ttcaatcagg cccagttctc
     4561 ggttcagcag cggtagtggc ggtgtcgtca tacagaaatg gtcggtcaga gaaaacggca
     4621 gggccggcag accgtccgcc cggcgcggcg ccgggcggtc agtgcgggcg tcaggcctgg
     4681 ggcggcacgt agcccgtggg catttccgaa cccgaaccaa agaaatggtt ttgcatttgc
     4741 tgggtcaggt attgccgggc gcgcgggtcg gccaggttga gacggttttc gttgatcagc
     4801 atggtttgca gcttcagcca cgcctgccag gcttccttgg agacggagtc gtacagcttt
     4861 ttgcccagtt cgccgggcat cggcggcaca tccaggccct cggcttcacg gccaagcttg
     4921 atacagttca ccatgcgggt catcgcaatt cctttttgac agtatacgga cagcgcgcgc
     4981 tgccatgatg acgaaacgat gacagcgtgc gtaaaaatac gacgggcaag cccgccgagt
     5041 cgcaacaggc agttttaagc gaaatcggcg cttggggcaa ggttttcgcg cgcagttggg
     5101 tggcgatggc ggtggctggc gggttcaggc ggttttggcc tgggcaatgc aggcggccag
     5161 ggccgcgcgg gcctgcgggc tgttgcgcca gcaactgccg cccaccaagg cagccacacg
     5221 ttgggcgagc acgtcggcgt cttctgtcgc caattgggca aagctgtgaa tgcccagttg
     5281 ctccagacgc agcaccacgg tggggccaac gcctttaaga gccagcaggg cggttttttc
     5341 ttcggcagaa aaagtctggg cagtcatggt gcggcaggca agtcatgaag acgtcattgt
     5401 gacatgtttg cctgccgagg cgaacccccg tggcgtcgca cggggatggg tatcaggccg
     5461 tggccgccag ctgtgcgcgc agttgtcgcg tggccgtcag catattggcc agcgcggctt
     5521 cggtttctgc ccagttgcgg gttttcaggc cgcagtccgg gttgacccac agccggcgcg
     5581 ccgggatcac cgccagcgcc ttgttcagca gcttgaccat ctcggcgctg tccgggatgc
     5641 gcggcgaatg gatgtcgtac acgccggggc cgatttcatt ggggtaggca aattcggcaa
     5701 aggcgtccag aagctccatg tccgagcgcg aggtttcgat ggtgatcacg tcggcgtcca
     5761 gcgcggcgat ggccggcagg atgtcgttga actccgagta acacatatgg gtgtggattt
     5821 gcgtggcgtc gtccaccccg gcggcagaca ggcgaaagct ctggccggcc cagtccagat
     5881 aggcatccca atcggcgcgc ttgagcggca ggccttcgcg gaaggctggt tcgtcgatct
     5941 ggatcacccg gatgccggcg ttttccagat ccagcacctc gtcgcggatg gccagggcga
     6001 tttgccggca cacctcggcg cgggccaggt cgtcacgcac aaacgaccat tgcaaaatgg
     6061 tcaccggacc ggtgagcatg cccttcatcg gccgctcggt cagcgactgg gcaaacaccg
     6121 accaggccac cgtcatcggc tgcgggcgcg acacatcgcc aaacaagacc ggcggcttga
     6181 cgcagcggct gccgtagctt tgcacccagc cgtggcgggt aaagacaaag ccgtccagct
     6241 gctcgccaaa gtattccacc atatcattgc gctcggcctc gccgtgcacc agcacatcca
     6301 ggcccaaggc ttcctgcttg cgcaccgcca gggtgatttc tgcgcgcatg gcggcgtcgt
     6361 agctggcggc gtccagcttg ccggcgcgcc aggcggcgcg ggcggcgcgg atgtcggcgg
     6421 tctgcgggaa agagccaatc gtcgtggtgg gcagcagcgg cagcttcagc cagtcatgct
     6481 gggcgcgagc gcgctgcggg taggcgctgc ggcgctggtc gtcgccgggg cgaatatcgg
     6541 ccaggcgctg gcgcaccgcc gggttgacca cgcgcgggct ggcgcggcgg ctgttgtggg
     6601 cgtggcggca ggcggccagt tcgttggcga tgctgtcggc gccgtggttc aggccgcgcg
     6661 ccagcagttt cagctcgttg agcttttgca cggcaaaggc cagccaggtt ttgacttcgg
     6721 cgtccagctc ggtttcctgc gccagatcca ccggcacatg caagagcgag cacgacggcg
     6781 ccagccacag ctgttcgccc cattgcgccg ccagcggggc cagttgcgtc aacagggcgt
     6841 tcaggtcggc gcgccagata ttgcgcccgt cgatcacccc cagcgacagc acccgctcac
     6901 gcggccagtc ggcgacaaag ccggccaatt gctcgggcgc gcgcaccacg tccagatgca
     6961 cgccggccac cggcaggcgt ttgagcaagt cgcgctgcgg cagcacgctg gcaaaatagc
     7021 tggccagcag caggcgcggc gcttgcgcgg ccagttgcgc ataggccggc tggtaggcgg
     7081 ccagccagtc ggccggcaaa tccaggccca gaatcggctc gtcgatctgc acccagtcca
     7141 cgccgtggat gctcagcgcg ctcagcagct cgccataggc gctcagcagc gccggcagca
     7201 ggctcaggcg gtccacgccg tccttgcttt tgcccagcca cagcaaggac agcggcccca
     7261 gcagcaccgg cttggcggcc aggcccagcg cgcgcgcttc ggccagcggc tccagcagcc
     7321 agcgcggctg ggcggcaaag gtggtgtggc ggtcaaattc cggggccaga tagtggtaat
     7381 tggtgtcaaa ccacttggtc atttccatcg ccggctggct ggcgttgccg cgcgccaggg
     7441 tgaattggtc ggccagggtg gcctgcgccg ggtcgatggc aaagcggccg ggcagcgcgc
     7501 ccagcagaaa cagggtggac agcacatggt cgtagcaggc aaagtccccc accgggatcc
     7561 agtccagccc ggcggctttt tgcgtcagcc agtggcgctg gcgcagcgtg cgggcggtgt
     7621 gctccagcgc ctccaggctg tcttcgccgc gccagtggcg ctccagggcg aatttcagtt
     7681 cgcgctgcgc gccaatgcgc ggaaagccga ggttatgaat cttgatcatt gtttatcctt
     7741 tacgccatga actaaatttg agtgacgcta ggatgccgga aggctgttgt atgatacaaa
     7801 ctcattattt tgatgaattc aatgaacaga attcatagag aacgccatgg cgacttccct
     7861 gcttgaactg cgccacctgc gcaccctgct ggcgctggcc gaatgcggca atgtctcggc
     7921 cgccgcccgc cggctgaatc tcacccagtc ggcgctgtcg caccagctga aaaccctgga
     7981 aagccactac ggctgcgcgc tggtggatcg ccgccgccag ccgctggcgc tcaccgccgc
     8041 cggcgagcgg ctgcacaccc tggcgcgcag cgtgctggcg caggtgcagc aggccgaacg
     8101 cgagctggcg cgcattgccc agggcgaggc cggccagctg cgcattgccg tggagtgcca
     8161 cacctgtttt gactggctga tgccggcgat ggacgatttt cgtggccact ggcccgaggt
     8221 ggagctggat atcgtgtcgg gctttcatgc cgacccggtg ggcctgctgc tcagccgccg
     8281 tgccgacctg gcggtggtgt cggcgccaga agacgaagcc gccgtgcgct accacccgct
     8341 gtttggctat gaaatggtgg ctgtgctggc gcgcgaccat ccgctggccg gcaaaccctg
     8401 gctgagcgcc gccgactttg cccgcgaaac cctgatcacc tacccggtgc cggacgacaa
     8461 gctggatctg gtctggcagg tgctgcgccc ggccggcatc aacccaccac gccgcaccac
     8521 cgagctgacc gtggccatgg tgcagctggt ggccagccgg cgcggcgtgg cggcgctgcc
     8581 gttctgggcg gtcaagcctt atctggagcg cggttatgtg caggcgctgc cgattggcga
     8641 gcacggcctg cgcagcgagc tatacgccgc cacccgcgcc gacgacgccg ggctggctta
     8701 tctggaagat tttttggtga cggtgcgcga gctgagtttt gccaccttag ccggcattcg
     8761 tccgctggc
//
LOCUS       DAOPYX010000114         8552 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_9851_length_8552_cov_4.863834, whole genome shotgun sequence.
ACCESSION   DAOPYX010000114 DAOPYX010000000
VERSION     DAOPYX010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 8552)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8552)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8552
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9851_length_8552_cov_4.863834"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     repeat_region   57..205
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=58..85
                     /rpt_unit_seq="tttctcagctgcctattcggcagttcac"
     gene            417..1409
                     /gene="cas1f"
                     /locus_tag="PKN55_07280"
     CDS             417..1409
                     /gene="cas1f"
                     /locus_tag="PKN55_07280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019959482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004520 - endodeoxyribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-F CRISPR-associated endonuclease Cas1f"
                     /protein_id="HNX71081.1"
                     /translation="MDEIRATDLKTILHSKRANLYYLEHCRVLVNGGRVEYVTDQGKQ
                     SLYWNIPIANTTTVLLGTGTSITQAAVRELAKAGVMLGFCGGGGTPLFAAQEHELEVA
                     WFAPQSEYRPTEYLQAWTRFWFDDGQRLAAAKLFQAERLAHIARQWPLRARAEPGFAC
                     EMDRLNGALEASRVRIEHAPDHTALLTEEARLSKILFRLACEATGYGDFTRAKGGAGS
                     DPANRFLDHGNYLAYGLAASATWVLGLPHGLAVLHGKTRRGGLVFDVADLVKDALILP
                     QAFVSAMRGDDEQAFRRACVEALTRAEALDAMIDTLKLTASRLGGAPAGQGGAV"
     gene            1406..>4684
                     /gene="cas3f"
                     /locus_tag="PKN55_07285"
     CDS             1406..>4684
                     /gene="cas3f"
                     /locus_tag="PKN55_07285"
                     /inference="COORDINATES: protein motif:HMM:TIGR02562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-F CRISPR-associated helicase Cas3f"
                     /protein_id="HNX71082.1"
                     /translation="MNILLISQCSKKALTETRRILDQFAERRGERTWQTPITQAGLDT
                     LRRLLRKSARRNTAVACHWIRGKDHSELLWIVGDASQFNADGATPTNRTTRKLTRHAR
                     ENHWHTLEVISQLACLAALLHDLGKACAAFQARLRAGQVASRNLYRHEWVSLRLFQAF
                     VGVGADDASWLGRLAEVDGHGPDDWLRGLLRDGLDPAAHQQPPLAGLPPLAQAVGWLM
                     LSHHRLPVAPSDKPEDRLGKRLDNLHPALLHDVLDKIQPGWNERPSPASAAEIAPYWQ
                     FSHGLPVADLAWRKRAARCARRLLDAHHPAAGAWLDDPYVMQLSRLTLMLADHHYSSL
                     TDPTQRRGLNPHYPLHANTGPGGALKQTLDEHLLGVAWHAGQIAHALPELAEQLPRLA
                     RHKGLQRHTSVARFQWQNRAADCAAAMRARSREHGAFIVNMASTGCGKTLGNARVMYA
                     LAEPTQGLRCAFALGLRTLTLQTGRAFRQQLQLGEHELAIQVGGAANRQLFEHYQEQA
                     EQSGSASSQDLLAEDSHVLFEGNDQHPLLQRLAHSRGMPALLGAPVLVCTVDHLIPAG
                     DSLRGGSQIAPMLRLMGSDLVLDEPDEFDIGDLPALTRLVFYAGLLGSRVLLSSATLP
                     PALVQGLFDAYLQGRRHFQRNRGERPQQPPAVCCAWFDEFQHSQHDCAEPDAFAHAHL
                     AFAQRRHARLAAAEPRRRAELWPLHLPVGAQLHEALAAELLRALPDLHHAHASRDPIS
                     GKRVSFGLVRMANIRPLVAVALALFRLGAPAGLRLHLCVYHSQFPLLLRSAIEQQLDG
                     ALDRRQPDAVFARADIRHSLDSHPEADQLFVVLASPVCETGRDFCVDWALAEPSSMRA
                     LIQLAGRVRRHRPGAVASANLRVFDHPLRHFAHPNQAAYCHPGFEQTEPAAYRLHSHC
                     LSELLEPGELDVVDARPRLLARKPLRTQTRLADLEHGRVQDLMCAPEASKPLTARELR
                     SGKRHAGPLNAASIWSLPRAWLTGVLPQQQPFREQTRPEVELCFLLEEDGEDYALHQF
                     RPELSPAHKPYVRVDGALLHRIDDAQLRGQGISLWGQTDYLAAVHQLAEGLDISPEEC
                     ARRFG"
     assembly_gap    4685..4784
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4785..5341)
                     /locus_tag="PKN55_07290"
     CDS             complement(<4785..5341)
                     /locus_tag="PKN55_07290"
                     /inference="COORDINATES: protein motif:HMM:NF020419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HNX71083.1"
                     /translation="MNRFTPCLSILPPAQQRLWPMLAQASKLGFVLYGGTAIALRLGH
                     RESVDFDFFSDRPLDREAIRAAFPFMANATTLQDQGDAWVALTPGDEQQARVKLSFFG
                     GIDFGRIGEPEQTDDGVLLVASLDDLMATKVKVVLQRAEAKDYRDVAAMVHAGVSLSH
                     GLAGARLLFGQNFQPSESLKALVYFA"
     gene            complement(5338..5637)
                     /locus_tag="PKN55_07295"
     CDS             complement(5338..5637)
                     /locus_tag="PKN55_07295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX71084.1"
                     /translation="MIQINPEILTPLAGKYIWWKTPAEAVAMPERVIAQVMNLGDYDD
                     VQWLAEQVGDDVLCDVLTHAEAGQFNARSWAYWHYRLGLAQVEQVPPLPVRRFQQ"
     gene            6105..7496
                     /gene="csy1"
                     /locus_tag="PKN55_07300"
     CDS             6105..7496
                     /gene="csy1"
                     /locus_tag="PKN55_07300"
                     /inference="COORDINATES: protein motif:HMM:TIGR02564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-F CRISPR-associated protein Csy1"
                     /protein_id="HNX71085.1"
                     /translation="MPENPSPFQHAIAAFIEERLAAKLAKLDDDDPKCVALREQHQYF
                     TWLDDAARRVRQIQLVTHTLKPIHPDARGSNLYAPPSTLADYPELGSHALLGMHADDV
                     VGNAAALDVYKLLKLQIDGRSLLDWLRDGHPDAVAALSDDADDAHTLAEAFVGIAQPR
                     TGGPASHTYAKQVFWLVGDDPLDDGQYHLLSPLYPSSLAHALFAVLNEHRYGEANKLA
                     REALRARRDHDQPFYSYPSLAVQKLGGSKPQNISQLNSERGGNNYLLSSLPPVWQTHK
                     VRPPTGASVFDLFGRHDDVHRLVNGLRHFLESSAPDRADGDAPGPKNNRYTRDIRDRY
                     YADLIDELVLFSEELQEQLPANWSAHADCVLHEAEQLWLDPYRCETDPKFCASWMAMD
                     WPAEVGRRFANWLNKQLERRLPLGEVEMRHWRKELLVDERLDGWARHLHRLRREQDAP
                     SHIPTRVATREAT"
     gene            7493..8488
                     /gene="csy2"
                     /locus_tag="PKN55_07305"
     CDS             7493..8488
                     /gene="csy2"
                     /locus_tag="PKN55_07305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012696553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-F CRISPR-associated protein Csy2"
                     /protein_id="HNX71086.1"
                     /translation="MSATLPDNDGLLILPRLRIQNANAISSPFTHGFPAITAFIGLMW
                     ALERKLAEQQLPLIFNSVGVICHDFAEQTQPGYVQTFRLTRNPLNQRGDPPAIVEHGR
                     MHLTLTLVFGVSGGFVELDANARHQLAECVAEQLSCMRVAGGSVLPPAEPRPGGRRQL
                     PRLEVLSQDSEERANNFRRLRRQWLPGFALVSRDELLASHLHTLRQHTPHASVLDAWL
                     DLSRFNWRPAERDNHGKVIWRHDRPAGAGWLVPIPVGYAALGELHPPGSVRDARDANT
                     PFRFVEGLYSLGQWISPHRLERPEQLLWFADSQPEAGHYRCRNAFADTPAGAPFA"
BASE COUNT         1529 a         2665 c         2614 g         1644 t
ORIGIN      
        1 ctaccgtaag ggaaggttgc gcggtgtatg atttttagct aatttcgcgg cagtgacttt
       61 ctcagctgcc tattcggcag ttcacatgac gacgccggca ggcagggtcg cgccgtattt
      121 ctcagctgcc tattcggcag ttcacggcat cagctctacc gtggtcggtg ctgttgcttt
      181 ctcagctgcc tattcggcag ttcactagac gctttcctcg ccaagtcctt gctgggtcag
      241 ccttttcccg gcgaggcgct cagtttaccc tcttttttgc cgtttctgta agtccttgat
      301 ttttcatccc tgccgcccag cccgcaaaaa aagggtctgc gcgctggaca gcggcggtgg
      361 catggcaagg tactatgtgg gtgtggcccg ctgggcgggc gtgtggaggc gtcaccatgg
      421 acgagattcg cgcaactgac ctgaaaacca tcctgcattc caagcgggcc aatctgtatt
      481 acctggagca ctgccgggtg ttggtgaatg gtgggcgggt ggagtatgtc accgaccaag
      541 gcaagcagtc tttgtactgg aatatcccga ttgccaacac caccacggtg ttgctgggca
      601 cgggcacttc aatcacccag gcggcggtgc gcgagctggc caaggcgggg gtgatgctgg
      661 ggttttgcgg gggcggcggc acgccgttgt ttgcggcgca agagcatgag ttggaggtag
      721 cctggtttgc gccgcaaagc gagtatcggc ccacggagta tcttcaggcg tggacgcgct
      781 tctggtttga tgatggccag cggctggcgg cggccaagct gtttcaagcc gaacgcctgg
      841 cgcatattgc ccggcagtgg ccgttgcggg cgcgcgccga gccagggttt gcctgcgaga
      901 tggaccgcct gaatggcgcg ctggaagcat cgcgcgtgcg cattgagcat gcgcccgacc
      961 acacggcgtt gctgaccgaa gaggcgcggc tgagcaaaat actgtttcgt ctggcctgcg
     1021 aggccaccgg gtatggcgac tttacccgcg ccaagggcgg tgcgggcagt gatccggcca
     1081 atcgttttct cgaccatggc aattatctgg cctatggttt ggccgccagc gccacctggg
     1141 tgctgggctt gccgcatggc ttggcggtgt tgcacggcaa aacccggcgc ggcggtttgg
     1201 tgtttgatgt ggccgatttg gtgaaagacg cgctgatttt gccgcaggct tttgtcagcg
     1261 ccatgcgcgg cgatgatgag caggcgtttc gtcgcgcctg tgtggaggcg ctcacccgcg
     1321 ccgaagcgct ggatgcgatg atcgacacgc tcaagcttac cgccagccgc ttgggtggcg
     1381 cgccggcggg ccaaggcggc gcggtatgaa tattttgctg atttcgcaat gcagcaaaaa
     1441 agcgctgacc gaaacccggc gcattcttga tcagtttgcc gagcggcgcg gcgagcgcac
     1501 ctggcaaaca ccgatcaccc aggccgggct cgacactttg cgccggctgc tgcgcaaaag
     1561 cgcgcggcgc aataccgccg tggcctgcca ctggatacgc ggcaaagacc atagcgagct
     1621 gttgtggatt gtgggcgacg ccagccagtt taacgccgac ggcgccacac ccaccaaccg
     1681 caccacacgc aagctcaccc gccatgccag agaaaaccat tggcacactt tagaggtgat
     1741 cagccaactg gcctgcctgg cggcgctgct gcatgacttg ggcaaggcgt gcgcggcgtt
     1801 tcaggcgcgg ttgcgcgccg gccaggtggc cagccgcaat ctgtatcggc acgaatgggt
     1861 gtccttgcgg ctgtttcagg cgtttgtagg cgtgggggca gatgacgcca gctggctggg
     1921 tcggctggcc gaagtggatg gtcacggccc ggatgactgg ctgcgcggct tgctgcgcga
     1981 cggcctggac cccgccgctc accaacagcc acccttggct ggcttgccgc cgctggcgca
     2041 ggcggtgggc tggttgatgc tcagccatca tcgtttgcca gtcgcgccgt ccgacaagcc
     2101 ggaagacagg ctgggcaagc gactggataa tctgcatccc gccctgctgc atgacgtgct
     2161 ggacaaaatc cagccgggct ggaacgagcg ccccagcccg gccagcgccg cagagattgc
     2221 cccatattgg caatttagcc acggcttgcc cgtggcggac ttggcttggc gcaagcgcgc
     2281 cgcccgttgc gcccgccggc tgctggatgc gcaccacccg gcggcgggcg catggctgga
     2341 tgacccctat gtgatgcagc tgagccggct gacgctgatg ctggctgacc atcattattc
     2401 cagcctcacc gaccccactc agcggcgtgg cttgaatccg cattatccgc tgcacgccaa
     2461 caccggcccg ggtggcgcgc tcaagcaaac gctggacgag catttgctgg gcgtggcctg
     2521 gcacgccggc cagattgccc acgccctgcc cgagctggcc gagcaactgc cgcgcctggc
     2581 ccgacacaag ggcttgcagc gccacaccag cgtggcgcgt tttcagtggc aaaaccgcgc
     2641 ggcggattgc gccgcagcca tgcgtgcacg cagccgcgaa cacggcgcat tcatcgtcaa
     2701 tatggcgtcc accggctgtg gcaaaaccct gggcaatgcg cgggtgatgt acgcgctggc
     2761 cgagccgaca caagggctgc gctgcgcgtt tgccctgggg ctgcgcacgc tgacgctgca
     2821 aaccgggcgg gcgtttcgtc agcaactgca actgggtgag catgagctgg ctattcaagt
     2881 cggtggtgct gccaatcgcc agctgttcga gcattatcaa gaacaggccg agcaatccgg
     2941 ctcggcctcc agtcaggatt tgctggcgga agacagccat gtgctctttg aaggcaatga
     3001 ccagcatccg ctgttgcagc ggctggcgca tagccggggc atgccggcgc tgctgggcgc
     3061 gccagtgctg gtgtgcacgg tggatcatct gattccagcc ggcgacagcc tgcgcggcgg
     3121 cagccagatt gcgccgatgc tgcggctgat gggcagcgat ctggtgttgg acgaaccaga
     3181 cgaattcgat atcggcgatt tgccagcgct gactcggctg gtgttttatg ccggcctgct
     3241 gggcagccgc gtgctgctgt cgtcggccac cttgccgcca gcgctggtgc aagggctatt
     3301 tgatgcttat ttacagggcc gtcggcattt tcagcgcaat cgcggcgagc gcccgcagca
     3361 gccgccagcg gtgtgctgcg cctggtttga cgagtttcag cacagccagc acgactgcgc
     3421 cgagcccgac gcctttgctc atgctcacct ggcgtttgcc cagcgtcgcc acgcccgact
     3481 ggctgccgcc gagccgcgcc gacgcgccga gctgtggccc ttgcatctgc cggtgggcgc
     3541 ccaattgcac gaagccctgg ccgccgagct gctgcgcgcc ttgcccgatt tgcaccatgc
     3601 ccacgccagc cgtgatccaa tcagcggcaa gcgggtaagc tttggcctgg tgcgcatggc
     3661 caatatccgg cccttggtgg cggtggcgct ggctttattc cgcttgggcg caccggctgg
     3721 cctgcgcctg catctgtgtg tgtatcactc gcagtttccg ttgctgctgc gctccgccat
     3781 cgaacagcaa ctggatggcg cgttggaccg tcgccagccc gacgccgtat ttgcccgcgc
     3841 cgatattcgc cacagcctgg acagccaccc agaagccgac cagctgtttg tggtgctggc
     3901 gtctccagtg tgtgaaaccg gccgggattt ctgcgtggac tgggcgctgg ccgagccttc
     3961 ttccatgcgc gccttgattc aactggctgg tcgtgtgcgt cgccatcgcc ctggcgccgt
     4021 ggccagcgcc aatctgcggg tgtttgacca ccccttgcgt cactttgccc accccaatca
     4081 agccgcctac tgccaccccg gctttgaaca aaccgaaccg gccgcttatc gtctgcacag
     4141 ccattgcctg agcgaactgc tggagcccgg cgagctggat gtggtggacg cccgcccgcg
     4201 tctgttggcg cgcaagccac tgcgcaccca aacccggctg gccgacctgg agcacgggcg
     4261 cgtgcaagac ctgatgtgcg cgccagaggc aagcaaaccg ctgactgccc gcgaactgcg
     4321 cagcggcaaa cgccatgccg gcccactgaa cgccgccagc atctggtcgc tgccacgcgc
     4381 ctggctgacc ggcgtgctgc cgcagcaaca accctttcgg gagcaaaccc ggccagaggt
     4441 cgagctgtgt tttttgctgg aagaagacgg cgaggattac gccctgcatc agtttcgccc
     4501 tgagctgagc ccggcgcaca agccctatgt gcgggtggat ggcgctctgc tgcaccgcat
     4561 tgacgacgca caactgcgcg gccagggcat cagcctgtgg ggccagaccg actacctcgc
     4621 cgccgtgcat caactggccg aggggctgga catctcacca gaggaatgcg ccagacggtt
     4681 tggcnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnngcaaag tacaccagcg
     4801 ctttcaggct ttcgctgggt tgaaaattct ggccgaacag caagcgggcg cctgccagcc
     4861 catgtgacag actgacgccg gcgtgaacca tggcggccac atcgcggtag tccttggctt
     4921 cggcgcgctg caacaccacc ttgactttgg ttgccatcaa atcatcaagg gacgccacca
     4981 gcaagacgcc atcatcggtc tgttctggtt cgccgatgcg cccaaaatca atgccgccaa
     5041 aaaaagacaa tttgacgcgc gcttgctgct catcgccagg tgtgagcgct acccacgcat
     5101 cgccctgatc ttgaagcgtc gtggcattgg ccataaaggg aaatgctgcg cgaatcgcct
     5161 ctcgatcaag aggccggtca gaaaaaaagt caaaatccac ggattcgcgg tgacccagcc
     5221 tcagcgcaat ggcagtgccg ccatacagta cgaaccccag ctttgatgct tgagccagca
     5281 tgggccaaag gcgctgttgt gcgggcggca gaatgctcag gcaaggggta aaccgattca
     5341 ttgttgaaat ctccgcaccg gcaatggcgg cacttgctcc acctgcgcca accccagtcg
     5401 gtaatgccag tatgcccagg aacgagcatt gaactggcct gcctcggcat gggtgagcac
     5461 atcgcacagc acgtcatcgc ctacttgttc ggctagccat tgcacatcgt cgtaatcgcc
     5521 cagattcatc acctgagcaa tcacccgctc cggcatggcc accgcttctg ccggggtttt
     5581 ccaccagatg tatttgccgg ctagcggcgt cagaatttct gggttgatct ggatcatata
     5641 gccctcgctc gataggcaca cgaataagat ttatcggcaa attgtgcatg gggtggctaa
     5701 gtgaagcaat gcccagaaaa aaccgccaga caagctggcg gttggggtgc ggcgctgcct
     5761 gtgcggcagt tcagggtgcg gcagaggttc gtcattctaa tagacgcatt tctcagctgc
     5821 ctgtgcggca gtttgacgca agcggatatt accactgctc ttgaggtttg gcgtgatagc
     5881 aacagataaa tattcaattt aatatacgca ggaatttgac aagtcatatc actaccgata
     5941 atctctatgt catcgctcac gcgtcatgtc actgagccct gactttccca caacaccagc
     6001 ggaggatgcc gccatgtagc cgttctgttt tcacctggtc tggccgcgcg gcatcctggc
     6061 cgatcttcgc gtggtttgtc gccaatcttt tcccggagcg attcatgccc gaaaaccctt
     6121 caccctttca gcacgccatt gccgccttca tcgaagagcg gctggccgcc aagctggcca
     6181 agctggacga cgatgatccc aagtgcgttg cattgcgtga gcagcaccaa tacttcacct
     6241 ggctggacga cgctgcccgg cgagtgcggc agattcaatt ggtcacccac accctgaaac
     6301 ccatccaccc cgatgcgcgc ggcagcaatc tgtatgcgcc gccgtcaacg ctggctgact
     6361 atcccgagct gggcagtcat gcgctgcttg ggatgcatgc tgacgatgtg gtgggcaatg
     6421 ccgccgccct ggatgtctac aagctgctga agctgcaaat agacggccgt tcgctgttgg
     6481 attggctgcg cgatggccac ccggacgctg tcgctgcgct cagcgatgac gcggacgatg
     6541 cgcacaccct ggccgaggca tttgtgggca ttgcccagcc acgcaccggg gggccggctt
     6601 ctcataccta cgccaagcaa gtgttctggc tggtgggcga cgatccgctg gacgatgggc
     6661 aatatcacct gctctcgccg ctgtacccca gctcactggc gcatgcgctg tttgccgtgc
     6721 tcaatgagca ccgctatggc gaagccaaca aactggcgcg cgaggctttg cgcgcccggc
     6781 gcgaccatga ccaaccgttt tattcctatc cgtcactggc cgtgcaaaag ttgggcggct
     6841 ccaagccaca aaatatcagc cagctgaaca gcgagcgtgg cggcaataac tacctgctca
     6901 gcagcctgcc gcccgtctgg caaactcaca aagtgcgccc acccacaggg gcgtcggtgt
     6961 ttgatttgtt tggccgccat gatgatgtgc ataggctggt gaacgggctg cgtcattttc
     7021 tggaaagcag cgcgccagat cgggctgatg gcgacgcccc cggccccaag aacaacagat
     7081 acacccgcga catccgcgac cgctattacg ctgatttgat tgatgagctg gtgttgttta
     7141 gcgaagagct gcaagaacaa ctgccggcta actggagcgc ccacgctgac tgcgtactgc
     7201 acgaagcaga acaactctgg ctggacccct accgctgcga aaccgacccc aaattttgcg
     7261 ccagctggat ggccatggac tggccggcag aagtgggtcg gcgctttgcc aactggctaa
     7321 acaagcagct ggagcggcgt ttgccattgg gcgaagtaga gatgcgccac tggcgcaagg
     7381 aactgctggt ggacgaacgg ctggatggct gggcgcgcca tctgcaccgg ctgcgtcgcg
     7441 agcaagatgc ccccagccat atccccacgc gcgtcgccac ccgggaggcc acatgagcgc
     7501 cactttgcct gataacgatg gcctgctgat tctgccgcgt ctgcgcatcc agaacgccaa
     7561 cgccatttcc agcccgttca cccatggctt tcccgccatc accgccttta ttggtctgat
     7621 gtgggcgctg gagcgcaagc tggctgagca gcaactgccg ctgattttca acagcgtggg
     7681 ggtgatctgc cacgattttg ccgagcaaac ccagcccggc tatgtgcaaa cttttcgtct
     7741 gacccgcaac ccgctgaacc agcgtggtga tccgcccgcg attgtcgaac acggccgcat
     7801 gcacctgaca ctgactctgg tgtttggcgt cagcggcggc tttgttgaac tggatgccaa
     7861 cgcccgccat cagctggccg agtgcgtggc cgaacaactg agctgcatgc gggtagctgg
     7921 cggcagcgtg ctgccccccg ccgagccacg ccccggcggt cgtcggcagc tgccccggct
     7981 ggaagtgctc agccaggata gcgaggagcg cgccaacaac tttcgtcgtt tgcgccggca
     8041 atggctgccc ggttttgccc tggtgagccg cgatgaacta ctggccagcc atctgcacac
     8101 cctgcgccag cataccccgc acgccagtgt gctagatgcc tggctggatc tgtcgcgctt
     8161 taactggcgt ccggcagagc gcgacaacca cggcaaggtg atctggcggc atgatcgccc
     8221 ggctggcgcc ggctggctgg tacccattcc ggtcggttac gccgcgctgg gtgagctgca
     8281 tccgcccggc agcgtgcgcg acgcccgcga cgccaatacg ccgttccgct ttgtcgaggg
     8341 gctgtatagc ctggggcaat ggatcagccc acatcggctg gaacgcccgg agcagctgct
     8401 atggtttgcc gacagccaac ccgaagcggg ccactatcgc tgccgcaacg cctttgccga
     8461 cacccccgct ggcgcaccat tcgcctgact cctgttgaac atcctgcaag gaaactttac
     8521 tgatggctct gaaaaccata tccgtcctgg ct
//
LOCUS       DAOPYX010000115         8535 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_9877_length_8535_cov_10.297642, whole genome shotgun sequence.
ACCESSION   DAOPYX010000115 DAOPYX010000000
VERSION     DAOPYX010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 8535)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8535)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8535
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9877_length_8535_cov_10.297642"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(42..2609)
                     /locus_tag="PKN55_07310"
     CDS             complement(42..2609)
                     /locus_tag="PKN55_07310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014088307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS-domain containing protein"
                     /protein_id="HNX71087.1"
                     /translation="MNDVNGDALRLAALERENAKLKKINAALMRRVEQGLADNGNSFT
                     FFQVAVELEHRIQERTRALEQAMAELEAANFALTQAKRAAETAQGRLDVAVDTIADGF
                     ALWQPDDTLARCNRRFLDLFPNQRGMIRPGLHFEDFVAAVVSDGGVLEARDDPFGWLQ
                     RRVAYHREPEGSLILSMADGTWQRISERRTGDGGRVGIYTDITDIKRQEMRLRESDLA
                     AHSLLLQATLNSIRQGIAVFDAHGALLAWNHRFSDLVGLSPQALAVGKRISPRERTLC
                     CPLSGALEMAGTRGRTLEVQYNPMPNGGFAMTYTDISERKAAEQALRDSEAKMRLITD
                     ALPALIAYVDNQQIYRFTNQGYEDWFGIPRSEINGRPMREVLGPRLFDGRRHYVERAL
                     EGRASVFELELPAPRRNIEFAKATFIPHFDPDHGGVLGFFALIQDITDIRRAARALEH
                     SKEQLEQRVAERTIELSHANSRLQEAIHAAEEAQRSKTRFFAAASHDLLQPLNAARLF
                     LASLAGQPLPPNAARLADNAGASLEAVDDLINTLLEISRLDAGAVETDMRHFPVAALL
                     DQLATEFAPLAEARGLSLRLHNCAAVVHSDWVLLGRVLRNFLSNAIRYTRHGSLLLGC
                     RRIDGGVRVGVWDQGAGIEPEKLPTIFGEFVRLSEHSSMCNKGMGLGLAIVHRLARAL
                     QHRLYVRSIPGQGSCFAIDIPYGRPAAVQFDAPHDDAPSANHPAQTGDALLLLIDNDP
                     AILEGMVTLLEDWDYQTLAASSAEAALTLLDAHGLTPDAILADYHLDDGATGSEAIRR
                     ICQHLGQNIPAALITADRSAEVRQEAEAAGWAWLGKPVRPARLRALLGHVLRRGEMG"
     gene            complement(2606..3754)
                     /gene="nosP"
                     /locus_tag="PKN55_07315"
     CDS             complement(2606..3754)
                     /gene="nosP"
                     /locus_tag="PKN55_07315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014088306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035991 - nitric oxide sensor activity
                     [Evidence IEA];
                     GO_process: GO:0046209 - nitric oxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing protein NosP"
                     /protein_id="HNX71088.1"
                     /translation="MNFAPRVRRGHTTLADVDAAVDELHAQLDQADSTLVLIFASPDY
                     PPEALAAALNRRFADTLVVGCTTAGEIGPGGYQHHSISAVSFAAPDFCAVAQRIDDLA
                     GMSLSDALGVAMGARHQLGVRSPFPIERRTFALLLIDGLSAREEQVASRLSRALGDIP
                     MFGGSAGDNMRFERTAVLIDGQFVSAAAALVLVSTPHPFEVFKSEHAAGYGERVVVTE
                     ADADRRIVTEINAEPAAAEYARLLGLAPEKLTPQAFAAHPLVLQVGGVPYVRSIQRVN
                     PDLSLSFFCAIDEGLVLSGTRAHDVVSNLARTFDGLRRRLGPLQIVLGCDCILRTLAE
                     HDSATRTRLSALLAANHVVGFNTYGEQFNAMHVNQTFTGIAIGYGLPA"
     gene            3916..4482
                     /locus_tag="PKN55_07320"
     CDS             3916..4482
                     /locus_tag="PKN55_07320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008952541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent catabolism-associated CXXCW motif
                     protein"
                     /protein_id="HNX71089.1"
                     /translation="MRRFSLIALLAVGLAGCAQLAAPELPPPPAGYRLSHYRAPTPAE
                     LPGAQRVDARQLAARLQAAPRPLLIDVLPAGRLTDSDGVSHWLPNAPRRNLPGSVWLP
                     NVGHGEPEPAMADWFARELARLSAGNRDAPLVFYCLRDCWMSWNAGKRALALGYRQVL
                     WFADGSDGWQEVGLPLEDAAPLPYTPPR"
     gene            complement(4500..5276)
                     /locus_tag="PKN55_07325"
     CDS             complement(4500..5276)
                     /locus_tag="PKN55_07325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008952542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNX71090.1"
                     /translation="MPVALTVLGAIVWRELLRFWQQRSRFFGALVRPLIWLIVFASGF
                     RAALGLSIIPPYETYITYDVYITPGLVGMIQLFNGMQHSLSLVYDREMGSMRVLLTSP
                     HRLSWLLTCRLLAGVVVSVVQVYGFLLIARLWGVDLPWMGFIWVFPALLITGVLLGAA
                     GMLISSWVNQIENFAGVMNFVVFPVFFLSTALYPLWKMQESSVWLYWVCAVNPFSSAV
                     ETLRFALYGQLALEPALVTLACAAVLLALAIRGYRPRQRA"
     gene            complement(5261..6004)
                     /locus_tag="PKN55_07330"
     CDS             complement(5261..6004)
                     /locus_tag="PKN55_07330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014088304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNX71091.1"
                     /translation="MSAPALRIAGLSHRYGARLALDGVSLNVAPGEFRVLLGPNGAGK
                     STLYGLLTRLLACQSGAIELFGQPLSAPAMAGLGVVFQQPTLDLDLTVEQNLAYFAAL
                     HGLRGSERRARIDAELQRMALVERRRERVRLLNGGHRRRVEIARALLHRPRLLLLDEA
                     TVGLDMPARRFLVEHVHRLAREEGLAVLWATHLIDEVRADDHIALLHQGRLLASGVAG
                     ELTAQAECDSLAQWFDRQTGKEDSACLWP"
     gene            complement(6001..6966)
                     /locus_tag="PKN55_07335"
     CDS             complement(6001..6966)
                     /locus_tag="PKN55_07335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008952544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YVTN family beta-propeller repeat protein"
                     /protein_id="HNX71092.1"
                     /translation="MTVFPRLACCAALLAALPALADTVYVSNEKDHTLSVIDSASMKV
                     LKTVPVGKRPRGITLSKDGTQLYICASDDNTVQVMDLATLKVIHNLPSGEDPEQFALS
                     PDGKQLYVANEDSNVVTIIDIASRKVAGQVDVGVEPEGMAVSPDGRWAVNTSETTNMV
                     HWIDTRTRKLVDNTLVDQRPRYARFTDDSKLLWVSSEVGGTVSVIDVATRKRIHTIRF
                     AIPGIPKDRIQPVGMRLTRDGRYAFVALGPANHVAVVDAKTYAPIKYLLVGRRVWHTA
                     LTGDEKQLWVSNGISNDVSVIDVDNLKVVKSIATGRYPWGVAIKP"
     gene            complement(7034..8197)
                     /locus_tag="PKN55_07340"
     CDS             complement(7034..8197)
                     /locus_tag="PKN55_07340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014088303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNX71093.1"
                     /translation="MLLRPFHLALTALCCAASSAWALTTVDIAYLEETRPPVATLANL
                     EPPPADLGRAGVELGIRDNDSTGKFLQQRFQLHSIQLPVGAPASEWLARLNKTKARLV
                     VANLPAATLETLLKQPGQEGVLWFNAGAADDALRQSRCRPNLLHTLPGRAMQADALAQ
                     YLVARRWKKWFLVTGPQPEDAAYAAAVRRAAKRFGGQIVGEKAWRYSHDARRTAQAEV
                     PTFTQVAEHDVMIVADERGDFGEYFPYRTWLPRPVAGTQGLVATGWHASAEQWGAVQL
                     QNRFRAYAKRPMQAADYAGWAAVRSVGEAAARTQSGDAKVLRDYIRSPEFELAAFKGR
                     SLSYRAWNGELRQPMLIAQPRALVSLSPQEGFLHPSSDLDTLGFDAPEVKCKP"
BASE COUNT         1446 a         2901 c         2848 g         1340 t
ORIGIN      
        1 agccactctt aatcccgcac acgcgccgcc ccaggcgccc atcaacccat ctcaccacgc
       61 cgcagcacat gccccaagag cgcgcgcagc cgcgccgggc gcaccggctt gcccagccag
      121 gcccagccgg cggcttcggc ttcctggcgc acttcggcgc tgcggtcggc ggtgatcagc
      181 gcggcgggga tgttttgccc caggtgctgg cagatgcggc gaatggcctc gctgccggtg
      241 gcgccgtcgt ccagatggta gtcggccagg atggcgtccg gggtcaggcc gtgggcgtcc
      301 agcagggtga gcgcggcctc ggcgctgctg gcggccaggg tttggtaatc ccagtcttcc
      361 agcagggtga ccatgccttc cagaatggcc gggtcgttgt cgatcagcag caataaggcg
      421 tcgccggttt gcgccgggtg gttggcgctg ggggcgtcgt cgtgcggggc gtcaaactgc
      481 accgccgccg ggcggccata cggaatatca atggcaaagc acgagccctg gccggggatg
      541 gagcgcacat acagccggtg ttgcagcgcg cgcgccagcc ggtggacgat ggccaggccc
      601 aggcccatgc ccttgttgca catgctgctg tgctcggaca ggcgcacgaa ctcgccaaag
      661 atggtgggca atttttctgg ctcgatgccc gcgccctgat cccacacccc cacgcgcacg
      721 ccgccgtcga tgcgtcggca gcccagcagc aggctgccat ggcgggtgta gcggatggcg
      781 ttggacaaaa agttgcgcag cacccggccc agcaacaccc agtcgctatg caccaccgcc
      841 gcgcagttgt gcaggcgcag gctcagcccg cgcgcctcgg ccagcggggc gaattcggtg
      901 gccagttggt ccagcagcgc cgccaccgga aagtggcgca tatcggtttc caccgcgccg
      961 gcgtccagcc gggagatttc cagcagggtg ttgatcaggt cgtccaccgc ctccagcgag
     1021 gcgccggcat tgtcggccag gcgggcggcg ttgggcggca gcggttggcc ggccagcgag
     1081 gccaaaaaca gccgggcggc gttcagcggt tgcaaaagat catggctggc ggcggcaaaa
     1141 aagcgggttt tgctgcgctg ggcttcttcg gcggcgtgga tggcctcttg caggcggctg
     1201 ttggcgtggc tgagctcaat ggtgcgctcg gccacgcgct gctcgagctg ttctttggaa
     1261 tgctccagcg cgcgggcggc gcggcggatg tcggtgatgt cctgaatcag ggcaaaaaag
     1321 cccagcacgc cgccgtggtc ggggtcgaag tgcggaataa acgtcgcctt ggcgaattcg
     1381 atattgcgcc ggggggcggg cagctccagc tcaaacaccg aggcgcggcc ttccagcgcg
     1441 cgctcgacat aatgccggcg gccgtcaaac aggcgcgggc ccagcacttc gcgcatgggg
     1501 cggccgttga tttcgctgcg cgggatgcca aaccagtctt cgtagccctg attggtgaag
     1561 cggtaaatct gctggttgtc cacataggca atcagcgccg gcagggcgtc ggtgatcagg
     1621 cgcatcttgg cttcgctgtc gcgcagcgcc tgctcggcgg ccttgcgctc gctgatgtcg
     1681 gtgtaggtca tggcaaagcc gccattgggc atcgggttgt attgcacttc cagcgtgcgc
     1741 ccgcgcgtgc cggccatttc cagcgcgcca gacaaggggc agcacagcgt gcgctcgcgc
     1801 gggctgatgc gcttgcccac cgccagcgcc tgcggcgaca agcccaccag atcagaaaaa
     1861 cggtgattcc acgccaacag cgcgccatgg gcgtcgaaca cggcaatgcc ctggcggatg
     1921 ctgttgagcg tggcttgcag cagcagcgaa tgcgcggcca gatcgctttc gcgcaggcgc
     1981 atttcctggc gcttgatgtc ggtgatgtcg gtgtaaatgc ccacccggcc gccgtcgccg
     2041 gtgcgccgct cgctgatgcg ctgccaggtg ccgtcggcca tcgacaaaat caggctgcct
     2101 tcgggttcgc ggtggtaggc gacgcggcgt tgcagccagc caaacgggtc gtcgcgcgct
     2161 tccagcacgc cgccgtcgct gaccacggcg gcgacaaaat cctcaaaatg caggccgggg
     2221 cggatcatgc cgcgctgatt gggaaacaaa tccagaaaac ggcgattgca gcgcgccagg
     2281 gtgtcgtccg gctgccacag ggcaaagccg tcggcaatgg tgtccaccgc cacatccagc
     2341 cggccttgcg cggtttcggc ggcgcgcttg gcctgggtca gggcaaagtt ggccgcttcc
     2401 agctcggcca tggcctgctc cagcgcgcgg gtgcgctcct gaatgcggtg ctccagctca
     2461 accgccacct gaaaaaaagt aaacgagttg ccattgtcgg ccaggccctg ctccacccgg
     2521 cgcatcagcg cggcgttgat ttttttcagt ttggcgtttt cccgctccag ggcggccaga
     2581 cgcagcgcgt cgccgttgac gtcgttcatg ccggaagtcc gtatccgatg gcaatgccgg
     2641 taaaagtctg gttgacatgc atggcgttga attgctcgcc ataggtgtta aagcccacca
     2701 catggttggc ggccaacagg gccgacagcc gggtgcgggt ggcgctgtcg tgctcggcca
     2761 gggtgcgcaa aatacagtcg cagcccagca caatctgcaa gggccccagc cgtcggcgca
     2821 ggccatcaaa ggtgcgcgcc agattgctga ccacgtcgtg ggcgcgggtg cccgacagca
     2881 ccaggccttc gtcgatggcg caaaaaaagc tcaggctcag gtccgggttc acccgctgaa
     2941 tcgaacgcac atagggcacg ccgcccacct gcaacaccag ggggtgcgcg gcaaacgcct
     3001 gcggggtgag tttttccggg gccaggccca agagccgggc gtattcggcg gcggccggct
     3061 cggcgttgat ttcggtgacg atgcgccggt cggcgtcggc ctcggtgacc accacccgct
     3121 cgccgtaacc cgccgcgtgc tcggatttga acacctcgaa cggatgcggc gtgctcacca
     3181 gcaccagcgc ggcggcggcg ctgacaaact ggccgtcgat cagcacggcg gtgcgctcaa
     3241 agcgcatatt gtcgccggcc gagccgccaa acatcgggat gtcgcccagc gcgcgcgaca
     3301 gccggctggc cacttgctct tcgcgcgcgg acaggccgtc gatcaacagc agggcaaagg
     3361 tgcgccgctc aatcggaaac ggcgagcgca cgcccagctg atggcgcgcg cccatggcca
     3421 cgcccagcgc gtccgacagg ctcatgccgg ccagatcgtc aatgcgctgg gccaccgcgc
     3481 aaaaatccgg cgcggcaaag ctcaccgccg aaatcgaatg gtgttgataa ccgccggggc
     3541 caatttcgcc ggccgtggtg cagcccacca ccagggtgtc ggcaaagcgc cggttcagcg
     3601 ccgccgccag cgcctcgggc gggtagtcgg gtgaggcaaa gatcagcacc agggtgctgt
     3661 cggcttgatc gagctgagcg tgcagctcgt ccacagcagc gtcaacgtcg gccagggtgg
     3721 tgtggccgcg ccgcacgcgc ggggcgaagt tcatgatggc gtgtctcctc gcgcctgccg
     3781 cccccgatcg ggcgccgggg tgtctgacaa acggtcggac cattttgccc gcccccccga
     3841 cgaaagtcga atgggcagcg ccctggcttt tgcccacgct gaatgtttgt tgttggtttg
     3901 cacaggagca tcgccatgcg ccggttttct ctgattgcct tgctggccgt gggcctggct
     3961 ggctgcgccc agctggccgc gcccgagctg ccgccgccgc ccgccggcta ccggctgagc
     4021 cactaccgcg cgcccacccc ggccgagctg cccggcgccc agcgggtgga cgcccgccaa
     4081 ctggccgccc ggctgcaagc ggcgccgcgc ccgctgttga ttgacgtgct gcccgctggc
     4141 cgcttgaccg acagcgacgg cgtcagccac tggctgccca atgcgccgcg ccgcaacctg
     4201 cccggcagcg tgtggctgcc caatgtcggc cacggcgaac ccgagccggc catggccgac
     4261 tggtttgccc gcgagctggc gcggctgagc gccggcaacc gcgacgcgcc gctggtgttt
     4321 tactgcctgc gcgattgctg gatgtcgtgg aacgccggca agcgcgcgct ggcgctgggc
     4381 taccgccagg tgctgtggtt tgccgacggc agcgacggct ggcaagaagt cggcctgccg
     4441 ctggaggacg ccgcgccgct gccctacacg ccgccgcgtt gacccgcgcc gcgcggcgct
     4501 catgcccgct ggcgcggccg gtagccgcga atggccagcg ccagcaacac cgccgcgcag
     4561 gccaaggtca ccaaggccgg ctccagcgcc agctggccat acagggcaaa gcgcagggtt
     4621 tccaccgccg aggaaaacgg attcaccgcg cacacccagt acagccagac gctggattcc
     4681 tgcattttcc acagcgggta cagcgcggtg gacaaaaaga acaccgggaa caccacaaaa
     4741 ttcatcaccc cggcaaagtt ctcgatctgg ttcacccacg acgaaatcaa catccccgcc
     4801 gcgcccaaca gcacgcccgt gatcaacagc gccggaaaca cccagataaa ccccatccac
     4861 ggcaggtcca cgccccacag ccgggcaatc agcaaaaagc catacacctg caccaccgac
     4921 accaccaccc cggccagcag ccggcaggtg agcagccagg acagccggtg cgggctggtc
     4981 agcagcacgc gcatgctgcc catctcgcgg tcgtagacca gcgacagcga atgctgcatg
     5041 ccgttgaaca actggatcat gccgaccagg ccgggggtga tgtacacgtc gtaggtgata
     5101 taggtttcgt agggcggaat aatcgacaag cccagcgcgg cgcgaaagcc cgaggcaaac
     5161 acgatcagcc agatcagcgg gcgcaccagc gcgccaaaaa aacgcgagcg ctgctgccaa
     5221 aagcgcagca attcgcgcca gacaatcgcg cccagcacgg tcagggccac aggcatgcgc
     5281 tgtcctcctt gccagtctgg cggtcaaacc attgcgccag cgaatcacat tcggcctggg
     5341 cggtcagctc gccggccacg ccgctggcca gcagccggcc ctggtgcaac agggcgatat
     5401 ggtcatcggc gcgcacttcg tcaatcaaat gggtggccca cagcacggcc aggccttctt
     5461 cccgcgccag ccggtgcaca tgctcgacca aaaaccgccg cgccggcatg tccaggccga
     5521 cggtggcttc gtccagcagc aacagacgcg ggcggtgcag cagggcgcgg gcgatttcca
     5581 cccggcggcg gtggccgcca ttgagcaggc gcacccgctc gcgccggcgc tcgaccagcg
     5641 ccatgcgctg caattcggcg tcgatgcgcg cgcgccgctc gctgccgcgc aggccatgca
     5701 gcgcggcaaa atacgccagg ttttgctcga cggtgagatc caggtccagc gtcggctgct
     5761 gaaacaccac gcccagcccg gccatggccg gggcggacaa gggctggcca aacagctcaa
     5821 tcgccccgct ctggcaggcc agcagccggg tgagcaagcc gtacagcgtg ctcttgccgg
     5881 cgccattggg ccccagcaac acgcgaaact cgccgggcgc cacattcagg ctgacgccat
     5941 ccagcgccag ccgcgcgccg tagcggtggc tgagcccggc aatgcgcaag gctggcgcgc
     6001 tcatggcttg atcgccacgc cccacggata acggccagtg gcaatcgact tgaccacttt
     6061 caggttatcc acatcgatca ccgacacatc attgctgatg ccattgctga cccacagctg
     6121 tttttcatcg ccggtcagcg cggtgtgcca cacccggcgg ccgaccagca ggtatttgat
     6181 tggcgcatag gttttggcgt ccaccaccgc cacatggttg gccgggccca gcgccacaaa
     6241 ggcatagcgg ccgtcgcggg tcaggcgcat gcccaccggc tggatgcggt ctttggggat
     6301 gccgggaatg gcaaagcgga tggtgtgaat gcgcttgcgg gtggccacat caatcaccga
     6361 cacggtgccg ccgacttccg agctgaccca cagcagcttg ctgtcgtcag taaagcgggc
     6421 gtagcgcggg cgctggtcca ccagggtgtt gtccaccagc ttgcgggtgc gggtgtcgat
     6481 ccagtgcacc atattggtgg tttccgaggt gttcaccgcc cagcggccat ccgggctgac
     6541 cgccatgcct tccggctcga cgccgacatc cacctggccg gccaccttgc ggctggcgat
     6601 gtcgatgatg gtcaccacat tgctgtcttc gttggccaca tacagctgct tgccgtccgg
     6661 cgacagggca aactgctcgg ggtcttcgcc cgagggcagg ttgtgaatca ccttcagcgt
     6721 ggccaggtcc atcacctgca cggtgttgtc gtcgctggcg cagatataca gctgcgtgcc
     6781 gtccttggac agggtgatgc cgcgcgggcg cttgcccacc ggcacggttt tcagcacctt
     6841 catgctggcg ctgtcgatca ccgacagggt gtggtctttt tcattggaca catacacggt
     6901 gtcggccagc gccggcaggg cggcaagcag ggcggcgcag caggccaggc gggggaatac
     6961 ggtcatcaga taaagctcct gtgcgtcagg gagaatcaca tgccgccgcg cccaggcgcg
     7021 gccaggcggg gggtcagggc ttgcacttca cttccggcgc atcaaaaccc agcgtgtcca
     7081 gatcgctgct ggggtgcaaa aacccctctt gcggcgacag gctgaccaag gcgcgcggct
     7141 gggcaatcag catcggctgg cgcaattcgc cgttccaggc gcgatagctc aggctgcggc
     7201 ccttgaacgc cgccagctcg aattccgggc tgcggatata gtcgcgcaac accttggcgt
     7261 cgcccgactg ggtgcgcgcg gcggcctcgc ccacgctgcg caccgccgcc cagccggcgt
     7321 aatccgccgc ctgcatcggc cgcttggcgt aggcgcggaa gcggttttgc agctgcaccg
     7381 cgccccattg ctcggcgctg gcgtgccagc cggtggccac caggccctgg gtgccggcca
     7441 ccgggcgcgg cagccaggtg cgatagggga aatactcgcc aaagtcgccg cgctcgtcgg
     7501 cgacaatcat cacatcgtgc tcggccacct gggtaaaggt gggcacttcg gcctgggcgg
     7561 tgcggcgggc gtcgtggctg tagcgccagg ctttttcgcc aacaatctgc ccgccaaagc
     7621 gcttggccgc gcggcgcacc gcggcggcgt aggcggcgtc ttccggctgc gggccggtca
     7681 ccagaaacca ttttttccag cgccgcgcca ccagatactg cgccagcgcg tcggcctgca
     7741 tcgcccggcc gggcagggtg tgcagcagat tgggccggca gcggctctgg cgcagcgcat
     7801 cgtcggcggc gccggcgtta aaccacagca cgccctcctg gcccggctgc ttgagcaggg
     7861 tttccagcgt ggcggccggc agattggcca ccaccagccg cgccttggtt ttgttcagcc
     7921 gcgccagcca ctcgctggcc ggcgcgccca ccggcagctg aatgctgtgc agctgaaaac
     7981 gctgctgcaa gaatttgccg gtggagtcat tgtcgcggat gcccagctcc accccggcgc
     8041 gccccagatc cgccggcggc ggctccagat tggccagcgt ggcgaccggc gggcgggttt
     8101 cttccaggta ggcgatgtcc accgtggtca aggcccaggc ggacgaagcc gcgcaacaca
     8161 gcgcggtcag ggcaagatga aacggtcgca acagcatggg cgcgctcgac aaatggggat
     8221 gctctgattg tggcaggctg gggatggctg tctattcgac taaggtgggg gagggcggcc
     8281 gacacaatca ggccatcacc gcagggccat tttggctgct gcgcccacac tcactctctc
     8341 cccccgctgt ccatcccacc aaagtctctc ccaccaaggg cggatggccg gggcgggggc
     8401 tttgccggta gaacctcttg cataacaaca tgccgacgcg cgcgcatgcg ccgcgcggtt
     8461 gcgcaccgga ggagaacacc atgcaaacgt ccgccccgcc tgcggcgccg ccgcgcgccg
     8521 cgctgcccga gcacc
//
LOCUS       DAOPYX010000116         8529 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_9894_length_8529_cov_8.430729, whole genome shotgun sequence.
ACCESSION   DAOPYX010000116 DAOPYX010000000
VERSION     DAOPYX010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 8529)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8529)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8529
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9894_length_8529_cov_8.430729"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            84..413
                     /gene="trxA"
                     /locus_tag="PKN55_07345"
     CDS             84..413
                     /gene="trxA"
                     /locus_tag="PKN55_07345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012697161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HNX71094.1"
                     /translation="MSSEKILHVSDASFEEDVLKASGPVLVDYWAEWCGPCKMIAPIL
                     DEVAEEYDGRLRVAKLNIDENEATPPKFGIRGIPTLMLFKDGSVVATKVGALSKSQLT
                     AFIDSNI"
     gene            541..1797
                     /gene="rho"
                     /locus_tag="PKN55_07350"
     CDS             541..1797
                     /gene="rho"
                     /locus_tag="PKN55_07350"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008954265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HNX71095.1"
                     /translation="MHLSDLKHLHVSQLVEMAIGNEIDGANRLRKQDLIFALLKNQAK
                     KGESIYGDGTLEVLPDGFGFLRSPDTSYLAGPDDIYVSPSQIRRFNLHTGDSIEGEIR
                     TPKEGERYFALVKVDRINGEAPEKSKHKILFENLTPLHPTRRLELERDIRAEENTTGR
                     VIDLISPIGKGQRGLLVAPPKSGKTVMLQHIAHAITANHPDVILIVLLIDERPEEVTE
                     MTRTVKGEVVASTFDEPATRHVQVAEMVIEKAKRLVEHKKDVVILLDSITRLARAYNT
                     VVPSSGKVLTGGVDANALQRPKRFFGAARNIEEGGSLTIIATALIDTGSRMDDVIYEE
                     FKGTGNMEIHLDRRMAEKRLYPAINVNRSGTRREELLIPQEQLQKIWVLRKLLYPMDD
                     LDAMEFLLDKIKATKSNQQFFDSMRR"
     gene            1990..3006
                     /gene="aroF"
                     /locus_tag="PKN55_07355"
     CDS             1990..3006
                     /gene="aroF"
                     /locus_tag="PKN55_07355"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008952727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HNX71096.1"
                     /translation="MIIVMQHTASAADLAAVVDKIRQAGLSEHVSRGAERTIVGAIGD
                     ERALSPAQFELMPGVERAMHVVKPYRIVSRESREGNTVVKVRGIPIGGEAIQVIAGPC
                     SVETPEQMDAAAEAVRAAGCRLMRGGAFKPRTSPYAFQGLGISGLAYLQNAARPRQLP
                     IVTELMDVRMLEVFLEYDVDVIQIGARNMQNFDLLKEVGKVNKPVILKRGLSATLSEW
                     LMAAEYIAAGGNHNIIFCERGIRTFETAYRNVLDITAIPALKRETHLPVIVDPSHAGG
                     KAWMVPSLALAAVAAGADGLLVEMHPNPCEAWCDADQALTPQELTALMSSLRGVAAAV
                     GRTL"
     gene            3137..3583
                     /locus_tag="PKN55_07360"
     CDS             3137..3583
                     /locus_tag="PKN55_07360"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014087322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HNX71097.1"
                     /translation="MSQGEIRVSLTQQSDYQFLIDFGAGLPQLLADEPAPLGHDQGPA
                     PSHMLVSAAANCLAASLLFSLRKFKQAPEPIRAEAVGRIERNAGGRLRMSTIEVTLHL
                     GRKAAEMDHLQRVLDTFEEFCTVSQSIKTGVDVQVAVHDADGVKIK"
     gene            complement(3790..4785)
                     /gene="mltB"
                     /locus_tag="PKN55_07365"
     CDS             complement(3790..4785)
                     /gene="mltB"
                     /locus_tag="PKN55_07365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014087860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HNX71098.1"
                     /translation="MLKRLSLAASLLIALGQAPAHAASLAGRADVDAYIDQISRQHGL
                     NRDEVRATLLAADTKPSILAIFDKPSTARPWYRFRPSFVNDGLARQGAAFWAKHAELL
                     QSASQIYGVDPAMIVAIIGMETGYGRNTGSFRVLDALATVAFDYPRRASYFQQELTEF
                     LLLAKEERRDPTEFKGSYAGAMGWPQFMPSSFRKWAVDGDADGHRDIWGNPADVIASV
                     AYYFQQHGWQRGGEMVVAATAPKDLADRLAQDKFNLHYTVAELRAMGVTPGGAVDEQA
                     RAVLFPLEVAPGEVEYWLGLQNFYTVTRYNKSTLYAMAAHQLADAIRQRRQPGAQ"
     gene            5066..5743
                     /gene="radC"
                     /locus_tag="PKN55_07370"
     CDS             5066..5743
                     /gene="radC"
                     /locus_tag="PKN55_07370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014088050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HNX71099.1"
                     /translation="MSIADWPEDQRPREKLLARGAASLSDAELLAIFLRTGIRGKSAV
                     DLARELLAHFDGSLSHLFAATPQAFSAVPGLGLAKYSQLAATRELAERAMAERLFAGD
                     VMNSPDVVRDYLRLRLHRREREVFVALCLSAQNQVLAMEELFQGSLTESRVYPREVAR
                     LALARNAAGVIIAHNHPSGSAKASEADIQLTRQLGQALALIDVRLLDHILVAGHQALS
                     FAEQGWM"
     gene            5812..6180
                     /locus_tag="PKN55_07375"
     CDS             5812..6180
                     /locus_tag="PKN55_07375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX71100.1"
                     /translation="MQERIVHSLYQRILADSNLTQRQKRQLLDDARQLLPDAHQHRAT
                     SWVIWLLALVVLSIPFTCAAHVLLVGSFPNDIPDGLLSLASAAGGALAGYLTPRQHRR
                     EAEPREEDEAWSAAERHTAD"
     gene            complement(6204..6482)
                     /locus_tag="PKN55_07380"
     CDS             complement(6204..6482)
                     /locus_tag="PKN55_07380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX71101.1"
                     /translation="MLSWFARRRPAAPTPRAPLFRLARLYALTSAVIGEYLWEERARW
                     QRAVEGAWTLEEYALLREPLAAALRATAGEASDGILAEFDALIEPPAA"
     gene            6789..8135
                     /gene="pcnB"
                     /locus_tag="PKN55_07385"
     CDS             6789..8135
                     /gene="pcnB"
                     /locus_tag="PKN55_07385"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018748979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004652 - polynucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006378 - mRNA polyadenylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HNX71102.1"
                     /translation="MIRKLLSRVFKLPERRPKPKVFPFTQHGVRRESISPAALKITSR
                     LQEAGFAAFVVGGAVRDLLLGQHPKDFDVATNATPEQVHHLFRRSRIIGRRFRIVHVM
                     VGAETIEVTTFRGGDDSVTDSEGRILNDNVFGSQEEDARRRDFTVNALFYNPSDETIV
                     DYHHGVRDVHARKLAMIGNPVQRYREDPVRMLRAVRLAAKLELRIDEPTRKPIAELAS
                     LLQNVPAARLFDEMLKMLFSGQAYQCVRMLRQEGLHQGIFPLLDVIAEESAGADFIRL
                     ALENTDRRIREDKPVSVGFLLATLLWGQVQREWTRRMDFGDKPIPALFDAMAAAERIQ
                     DDQLAIPRRFATTMREIWALQPRFDNRDGSRPYRLLEQPRFRAGYDFLALRAAVGEVP
                     QALVDWWTAFQEGDEAQRQALIAAARHSGEARTGAKKRRRRKKKPNGQGQPMDDGE"
     gene            8132..>8529
                     /gene="folK"
                     /locus_tag="PKN55_07390"
     CDS             8132..>8529
                     /gene="folK"
                     /locus_tag="PKN55_07390"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014087983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HNX71103.1"
                     /translation="MSVTAYIALGSNLQQPAEQVRAALAALAALPECSGLRASSLYVT
                     APVGYADQPDFINAVARLDTTLSALALLAQLQALEAQFGRERSFRNAPRVLDLDLLLY
                     GDQRIDTLELTVPHPRMHERAFVLAPLAELA"
BASE COUNT         1644 a         2751 c         2641 g         1493 t
ORIGIN      
        1 caggcgcgcc aggctgccgg catggcgatg cggcgaaccc gtttcactcc cccctatttg
       61 ttccgctttt ggagatgcat ataatgagca gcgaaaagat tttgcacgtc agcgacgcca
      121 gctttgaaga ggatgtgctg aaagccagtg gcccggtgct ggtggactac tgggccgaat
      181 ggtgtggccc gtgcaaaatg atcgccccga ttctggacga agtggctgaa gagtatgatg
      241 gccgcctgcg cgtggccaag ctcaatatcg acgagaacga agccaccccg cccaagtttg
      301 gcatccgtgg catccccacc ctgatgctgt tcaaggacgg cagcgtggtg gccaccaagg
      361 tgggcgcgct gtccaagagc cagctgaccg cctttattga cagcaacatc taaacggtct
      421 atcctcgttc tgtcatgttc gatcatggat ggcgcaatgc cgtccatggt tctcccgcac
      481 cttgccttga ttccccacgt cttttccgat cactctttta tttagagtcg acctcccctt
      541 atgcatttat ccgacttaaa acatctgcat gtcagccaac tggttgaaat ggccatcggc
      601 aacgaaattg atggcgccaa ccgcttgcgc aaacaggacc tcattttcgc cctgctgaaa
      661 aaccaggcca aaaagggcga aagcatttat ggcgacggca cgcttgaggt tctgcctgac
      721 ggattcggct ttttgcgcag ccccgacacc tcctacctgg ctggcccgga cgatatttat
      781 gtctcgccct cgcaaatccg ccgcttcaat ctgcacactg gcgacagcat cgaaggcgaa
      841 atccgcaccc ccaaggaagg cgagcgctac tttgcgctgg tgaaagtcga ccgcatcaat
      901 ggtgaggcgc cggaaaagtc caagcacaag attctgtttg aaaacctcac cccgctgcat
      961 cccacccgcc gcctggagct ggagcgcgac atccgcgccg aagaaaacac caccggccgg
     1021 gtcatcgacc tgatttctcc catcggcaaa ggccagcgcg gcctgctggt ggcgccgccg
     1081 aaatccggta aaacggtgat gctgcaacac atcgcccacg ccatcaccgc caaccacccc
     1141 gacgtgatcc tcatcgtgct gctgatcgac gagcgccccg aggaagtcac cgaaatgacc
     1201 cgcacggtca agggtgaagt ggtggcctcc accttcgacg aaccagccac ccgccatgtg
     1261 caggttgccg aaatggtcat cgaaaaagcc aagcgcctgg tggagcacaa aaaagacgtg
     1321 gtgattctgc tggattccat cacccgtctg gcccgcgcct acaacaccgt ggtgcccagc
     1381 tcgggcaagg tgctgaccgg cggcgtggac gccaacgcgc tgcaacgccc caagcgcttt
     1441 tttggcgccg cgcgcaatat cgaagaaggc ggcagcctga ccatcatcgc caccgcgctg
     1501 atcgacaccg gcagccgcat ggacgatgtg atttacgaag aattcaaggg caccggcaat
     1561 atggaaatcc atctggatcg ccggatggcc gaaaaacgcc tgtacccggc gattaacgtc
     1621 aaccgctccg gcacccgccg cgaagagctg ctgatcccgc aagaacagct gcaaaaaatc
     1681 tgggtgctgc gcaagctgct ctacccgatg gacgatctgg acgcgatgga attcctgctg
     1741 gacaagatca aggccaccaa gagcaatcag caattcttcg actcgatgcg tcgctgattc
     1801 tgcctggctg tcctgccttg ccacacgccg cccgcttgat tgccggcggc gtgtgtgttt
     1861 tggtgccggc cagcttgccc acaacactca gtgcagcaag accaggcgag tgatatcggc
     1921 ggcgcttggt agtcattccc gcgccgggcc gttatccttg acgcttctga atccgttgga
     1981 cagcacgtca tgatcatcgt catgcaacac acggccagcg ccgccgatct ggcggcggtg
     2041 gtggacaaaa tccgccaggc cggtttaagc gaacacgtct ctcgcggcgc cgagcgcacc
     2101 attgtcggcg ccatcggcga cgaacgcgcc ttgagcccgg cgcagtttga actgatgccc
     2161 ggcgtcgagc gcgccatgca tgtggtcaaa ccctatcgca tcgtctcgcg cgaaagccgc
     2221 gagggcaaca ccgtggtcaa ggtgcgcggc atcccgattg gcggcgaagc catccaggtg
     2281 attgccgggc cgtgttcggt ggaaaccccc gaacaaatgg acgccgccgc cgaggcggtg
     2341 cgcgccgctg gctgccggct gatgcgcggc ggcgccttca agccgcgcac cagcccctat
     2401 gcctttcagg ggttgggaat cagtgggctg gcctatctgc aaaacgccgc gcgcccgcgc
     2461 cagctgccga ttgtcaccga gctgatggac gtgcgcatgc tggaggtgtt tctggaatac
     2521 gacgtggacg tgatccagat tggcgcgcgc aatatgcaaa actttgatct gctgaaagaa
     2581 gtcggcaagg tcaacaagcc ggtgattctc aagcgcggct tgtccgccac gctcagcgaa
     2641 tggctgatgg cggccgaata catcgccgca ggcggcaacc acaatattat tttttgcgag
     2701 cgcggcatcc gcaccttcga aaccgcctac cgcaatgtgc tggacatcac cgccatcccg
     2761 gcgctgaagc gggaaaccca cctgccggtg attgtcgatc ccagccatgc cggcggcaaa
     2821 gcctggatgg tgccttcatt ggcgctggcg gcagtggcgg ccggcgccga tggcctgctg
     2881 gtggaaatgc accccaaccc ctgcgaagcc tggtgcgacg ccgaccaggc gctcaccccg
     2941 caagaactca ccgcgctgat gagctcgctg cgcggcgtag ccgccgccgt cggccgcacg
     3001 ctgtaaaaca ccacacaata aaatataata actgcgcgtt cagcggccag gccgccgagc
     3061 gacagacaac agcgccggcc catggcagcc tgggccggcg cacacccaca ccgctgccac
     3121 cgacaaagga cacaccatga gccagggcga aatccgcgtt tctctcaccc agcaatccga
     3181 ctatcaattc ttgatcgact ttggcgctgg cctgccgcaa ctgctggccg acgagcccgc
     3241 gccgctgggc cacgaccaag gcccggcccc cagccatatg ctggtgtcgg ccgccgccaa
     3301 ctgcctggcc gccagcctgt tgttttcact gcgcaaattc aagcaagccc ccgagccgat
     3361 tcgcgccgag gcagtgggcc gcatcgaacg caacgccggc ggccgcctgc gcatgagcac
     3421 cattgaagtc accctgcacc tgggccgcaa ggcggcagag atggaccacc tgcaacgcgt
     3481 gctggacacc tttgaagagt tttgtacggt gtcgcaaagc atcaagaccg gggtggacgt
     3541 gcaggtggcg gtgcatgatg ccgatggggt gaagatcaag tgatcacagg atgcagccag
     3601 aggcaagatc aatccatctg gcttgcgtcc acagcccgtg caaaaggccg cagcaaacca
     3661 agccacgcca ggcttcacac cgctgacaac actcagcaaa gcacgcccgg ctagacgtgg
     3721 gtggccgcag acacgagcaa gcggacagca agaccacacc cacgtcaggc gagcttgctg
     3781 agcctggctt tactgcgcgc ccggctggcg gcgctggcgg atggcgtcgg ccagttggtg
     3841 ggcggccatg gcgtatagcg tgcttttgtt gtagcgggtg acggtgtaga agttctgcaa
     3901 gcccagccag tattccacct cgcccggcgc cacttccagc ggaaacagca cggcgcgggc
     3961 ttgttcgtcc acggcgccgc caggggtcac gcccatggcg cgcagttcgg ccacggtgta
     4021 gtgcagattg aatttgtctt gcgccagacg gtcggccagg tcttttggcg cggtggcggc
     4081 caccaccatt tcgccgccgc gctgccagcc gtgctgctga aagtaatacg ccacgctggc
     4141 gatcacatcc gccgggttgc cccagatgtc gcggtggcca tcggcgtcgc cgtccactgc
     4201 ccatttgcga aaactggacg gcatgaactg cggccagccc atcgccccgg cgtaagagcc
     4261 tttgaattcg gtcgggtcgc ggcgttcttc tttggccagc aacagaaact cggttaattc
     4321 ctgctgaaaa taactggcgc ggcgcgggta atcaaacgcc acggtggcca gtgcatccag
     4381 cacacggaag ctgccggtat tgcggccgta gccggtttcc atgccgatga tggccacgat
     4441 catggccggg tctaccccgt aaatctggct ggcgctttgc agcagctcgg catgcttggc
     4501 ccaaaaggcc gcgccctggc gcgccaggcc atcgttgaca aagctggggc gaaagcgata
     4561 ccacggccgc gcggtggagg gtttgtcaaa aatcgccaga atcgacggct tggtgtcggc
     4621 ggccagcagg gtggcgcgca cttcgtcgcg attcaggccg tgctggcggc tgatctggtc
     4681 gatataggcg tccacatcgg cgcggccagc caggctggcg gcatgggcag gcgcttggcc
     4741 aagtgcaatc aacaggctgg cggcaagact caggcgtttc agcatggggg ttccggctag
     4801 tttgggagtt gtaaaaaaat cagctggcgt gaccttgcgc cataccagca gccactatgg
     4861 taacaccgaa caccgccccc tccaccgcat cccgctggcc cacgcgacat ctgtcttgcg
     4921 catgacatat gccatgcgcg atactcaagc gctgcccagc aaaacctgcg aagccatcct
     4981 gaccgaggag catgtcgata gcagggcagc aatgcgcgcc agggcgtagt gagtggcgca
     5041 tagaaaaccg ccgggggagg gcaatatgtc gatagccgat tggccagaag accagcgtcc
     5101 gcgcgaaaaa ctgctggcgc gcggcgccgc cagcctcagc gacgccgagt tgctggcgat
     5161 ttttttgcgc acggggattc gcggcaaaag cgcggtggac ctggcgcgcg agctgctggc
     5221 gcactttgac ggctcgctgt cgcatttgtt cgccgccacg ccgcaggcgt tttctgcggt
     5281 gcccggcctg gggctggcca aatacagcca gttggccgcc acccgcgagc tggccgagcg
     5341 cgccatggcc gagcggctgt ttgccggcga cgtgatgaac agcccggatg tggtgcgcga
     5401 ttatctgcgc ctgcgtctgc accgccgtga gcgggaggtg tttgtcgcgc tgtgtctgtc
     5461 ggcgcaaaat caggtgctgg cgatggaaga gctgtttcag ggctcgctga ccgaaagccg
     5521 ggtatacccg cgcgaggtgg cgcggctggc gctggcgcgc aacgccgccg gggtgatcat
     5581 cgcccacaat cacccctccg gcagcgccaa ggccagcgag gcggatattc agctcacccg
     5641 ccagctgggg caggcgctgg cgctgattga tgtgcgtctg ctcgatcata ttctggtggc
     5701 gggccatcag gcgctgtcgt ttgccgaaca aggctggatg tagatccggc ttcacaagcg
     5761 cgttggcgcg gcgttctgtc gatcagtctt gttactgaca cgggccggtt tatgcaagaa
     5821 cgcattgtcc attcgctcta ccagcgcatt ctcgccgaca gcaatctgac gcaacggcaa
     5881 aaacgccagc tgcttgacga cgcccgtcag ctgctgcccg acgcccacca gcatcgcgcc
     5941 accagctggg tgatctggct gctggcgctg gtggtgttgt cgattccgtt tacctgcgcc
     6001 gcccatgtgc tgctggtggg cagttttccc aatgatattc ctgatgggct attgtcgctg
     6061 gcctcggcgg cgggcggcgc gctggccggg tatctgacgc cgcgccagca tcggcgcgag
     6121 gcagagccac gggaggaaga tgaagcgtgg tctgccgcag aaaggcacac cgcagattga
     6181 gcacacctta cgctgagcgc gcctcacgcc gctggcggtt caatcaaggc gtcaaactcg
     6241 gccaggatgc catcgctggc ttcgccggca gtggcgcgca gggcggcggc cagcggttcg
     6301 cgcagcagcg cgtattcttc cagcgtccag gcgccttcta ccgcgcgttg ccagcgcgcg
     6361 cgctcttccc acaggtattc gccaatcacc gcgctggtca gggcgtacag ccgggccagg
     6421 cgaaacaggg gcgcgcgcgg ggtgggcgcg gcagggcggc ggcgggcaaa ccaggacagc
     6481 atggcgatct cctgtgggac acaccggccc ggacggaagg tgcaggcggc ggcaagttca
     6541 acttaatcaa ataataacaa ttatcattta gatgtgcaag ccactgaatg tcagccataa
     6601 gccagccggg cctggcccct cgcgccaggc cgcaaaacag tcgtttgtgc ggggtaaaac
     6661 atcgccagcg gccatgctcc cccacccgcc cagccccacg catgccacaa gaatggcgaa
     6721 ttcccgcagg aagggcgcgc aaggcggtat aatcatcgcc ttgttttgcc actgttgatc
     6781 tgaaagcgat gattcgcaag ctcctaagcc gcgtgttcaa actccccgag cgtcgcccca
     6841 aacccaaggt gttcccgttc acccagcatg gggtgcgccg cgagagcatc agtccggccg
     6901 cgctgaaaat cacctcccgc ctgcaagagg ccggctttgc cgcctttgtg gtgggcggcg
     6961 ccgtgcgtga cctgctgttg ggccagcacc caaaagattt cgacgtggcc accaacgcca
     7021 cgccggagca ggtgcaccat ctgtttcgcc gctcgcgcat tattggccgg cgctttcgca
     7081 tcgtccatgt gatggtcggc gcggaaacca tcgaagtgac cacctttcgt ggcggcgacg
     7141 actcggtgac cgacagcgag ggccgcatcc tcaacgacaa tgtgttcggc agccaggaag
     7201 aagacgcccg tcggcgtgat ttcaccgtca acgcgctgtt ttacaacccc agcgacgaaa
     7261 ccattgtcga ttatcaccac ggcgtgcgcg atgtgcacgc gcgcaagctg gcgatgattg
     7321 gcaacccggt gcagcgctac cgcgaagacc cggtgcgcat gctgcgcgcg gtgcggctgg
     7381 cggccaagct ggaactgcgc atcgacgagc ccacccgcaa gccgattgcc gagctggcct
     7441 cgctgctgca aaacgtgccg gcggcgcggc tgttcgacga aatgctgaaa atgctgttct
     7501 ccggccaggc ttaccagtgc gtgcgcatgc tgcgtcagga aggcctgcac caggggattt
     7561 ttccgctgct ggacgtgatt gccgaagaat ccgccggcgc cgactttatc cgcctggcgc
     7621 tggaaaacac cgaccggcgc atccgcgagg acaaacccgt ctcggtgggc ttcttgctgg
     7681 ccaccctgct gtggggccag gtgcagcgcg aatggacgcg gcgcatggat tttggcgaca
     7741 agccgattcc ggcgctgttt gacgccatgg ccgccgccga gcgcatccag gacgaccaac
     7801 tggccattcc gcgccgcttt gccaccacca tgcgggaaat ctgggcgctg caaccgcgct
     7861 ttgacaaccg cgacggcagc cgcccctatc gcctgctgga gcagccgcgc tttcgcgccg
     7921 gctacgactt cctggcgctg cgcgcggcgg tgggcgaagt gccgcaggcg ctggtggatt
     7981 ggtggacggc atttcaggag ggcgacgaag cccagcgcca ggcgctgatt gccgccgccc
     8041 gccacagcgg cgaggcgcgc accggcgcca aaaagcgccg ccgccgcaag aaaaaaccca
     8101 atggccaagg ccagccgatg gacgacggcg aatgagcgtc accgcctata tcgccctggg
     8161 cagcaatctg caacagcctg ccgagcaagt gcgcgccgcg ctggcggcgc tggcggcgct
     8221 gcctgagtgc agcgggctgc gcgcctcctc gctgtatgtc accgcgccag tgggctacgc
     8281 tgaccaaccg gactttatca acgccgtcgc ccgtctcgac accacgctgt ccgccctggc
     8341 gctgctggcg cagttgcagg cgctggaggc gcagtttggc cgcgagcgca gttttcgcaa
     8401 tgcgccgcgc gtgcttgatc tggacttgct gctgtatggc gaccagcgca tcgacacact
     8461 ggaactgacc gtgccgcacc cgcgcatgca cgagcgcgcc tttgtgctgg cgccgctggc
     8521 cgagctggc
//
LOCUS       DAOPYX010000117         8523 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_9905_length_8523_cov_8.634979, whole genome shotgun sequence.
ACCESSION   DAOPYX010000117 DAOPYX010000000
VERSION     DAOPYX010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 8523)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8523)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8523
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9905_length_8523_cov_8.634979"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            227..400
                     /gene="ccoS"
                     /locus_tag="PKN55_07395"
     CDS             227..400
                     /gene="ccoS"
                     /locus_tag="PKN55_07395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003822854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome oxidase assembly protein
                     CcoS"
                     /protein_id="HNX71104.1"
                     /translation="MESLYLLIPFSILLAFVIGGLFWWATRSGQFDDLEGPGHRILMD
                     DDSTKPDNKQPPP"
     gene            440..1729
                     /locus_tag="PKN55_07400"
     CDS             440..1729
                     /locus_tag="PKN55_07400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003790766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNX71105.1"
                     /translation="MSSTFAFFASRRFLPLFSTQFLGAFNDNLLKTAFLVWVSYAGLS
                     FAGLPAAQIVNLASGLFILPFFLFSATAGRLAEKIDKARVARWVKLSELAIMLLAALG
                     FSQHHAGVLLLALFLMGAHSTFFGPVKYAMLPQHLRPHELIGGNGLIEMGTFIAILLG
                     QMAGGMLALHSLPLTIALLMLAAALGWASSRTIPPAPPSAPDLPLHLNLWRDTADLLC
                     QARRTPALWTAMLGISWFWLLGAVYLTQLPTFTRLHLGGDPQVYTLILAVFSVAIGLG
                     SMLCARLSHAQLRLGWVGVGLLGMTVAGLDLYAASHARHSGELMGLATYLARPAAWRQ
                     LLDFALLGGFGGLFTVPLYTWLQTASPEDFRAQAIAANNIVNGLFMVAAAALSAVLLA
                     WLDSIALLFLCVAVCNLPALWWLARREPMLWPWRRQG"
     gene            complement(1787..2119)
                     /locus_tag="PKN55_07405"
     CDS             complement(1787..2119)
                     /locus_tag="PKN55_07405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX71106.1"
                     /translation="MSCLRVGQLAIGLASASALAQGGGLDAARCTFNGKPLAGRVQVV
                     NSFPDVRVQVVASFPDMRVQWVNSFPDRCGRWQQVESFPDVRIQYVDSFPDVRVQFVE
                     SFPGAQPR"
     gene            2273..2665
                     /locus_tag="PKN55_07410"
     CDS             2273..2665
                     /locus_tag="PKN55_07410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX71107.1"
                     /translation="MPVTPLDRASAARVAALPSQAVSSQPFAELLHSKQTRIGLNPAS
                     VLDALSPAARRAFDAAMRAAPTLAGQDVGAQAVSSAPAVLNAALDSLLNPSSPDFTPE
                     AVKTALRQLQASLAAGKAPTTPSETPSA"
     gene            2782..3471
                     /gene="elbB"
                     /locus_tag="PKN55_07415"
     CDS             2782..3471
                     /gene="elbB"
                     /locus_tag="PKN55_07415"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014087320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HNX71108.1"
                     /translation="MKSSLPVAVVLSGCGVYDGTEITEAVGLLIALSQAGFSYRCYAP
                     VREQYHVVDHFKGAPADGARNILTESARIARGAIQPLSAFKAAEHCALAFPGGFGAAK
                     NLTTFIDQGDKAQLYADVNSAVTDAMARKLPLLALCAAPLVLGIAARDAGLSGVRLTF
                     GDCEGPAMNAALATWGQTHVVTPLEQACVDSKHRMISAPAYMYGDANPAGVFASIQAA
                     VKALDEMLAAA"
     gene            complement(3567..4952)
                     /gene="gltX"
                     /locus_tag="PKN55_07420"
     CDS             complement(3567..4952)
                     /gene="gltX"
                     /locus_tag="PKN55_07420"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011135488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HNX71109.1"
                     /translation="MIRTRFAPSPTGFLHIGGVRTALFSWAYARKHGGTFVLRIEDTD
                     LERSTPESVQAILDGMHWVGLDYDEGPFYQTQRFDRYKDVIQQLLASGHAYLCYCSKE
                     ELDAMRAEQEARGEKPRYDRRWRPEDGKTLPTPPAGVPPVVRFKTPLSGSVAWDDAVK
                     GRIEFQNEELDDLIIARPDGSPTYNFCVVVDDWDMNITHVIRGDDHVNNTPRQINILK
                     ALGAPLPVYGHLPMILNEDGHKMSKRRDAVSVVDYEGKGILPEALLNYLARLGWGHGD
                     EEFFNMDQFVEWFSLEAVSPSASRFNQEKFLWLNAQHIKAADNARLAELITPRLTAAG
                     ISTAQGPALAEVVALVKERVQELNALARECDYFYQPRQPQQAELDKHILPDTRERMAR
                     FAAQLAALPEWNTEAIHGLFKPFCAEEGIKMGQLGMPLRVLVCGTTQTPSVDAVLALI
                     GREQVLARLAG"
     gene            complement(5188..5640)
                     /locus_tag="PKN55_07425"
     CDS             complement(5188..5640)
                     /locus_tag="PKN55_07425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HNX71110.1"
                     /translation="MHHPYMWFVLAALALVAELILGTFYFLVIGISMMLVGLGGVWGY
                     LDGNVQGLLVVTLSGLGCWRLWRYQQIQRQRPLEALEVIGQPVVVEQWLSPQQARVQW
                     RGAHWDARLAKGHASLFGEATYIISAQEGNLLWIEPFTDIGSPPVKLH"
     gene            5982..6515
                     /locus_tag="PKN55_07430"
     CDS             5982..6515
                     /locus_tag="PKN55_07430"
                     /inference="COORDINATES: protein motif:HMM:NF021361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2059 domain-containing protein"
                     /protein_id="HNX71111.1"
                     /translation="MKTSLLAISLLLVSHTLPVWASEAPASELSVREMLHITQTEQNY
                     RDTMVQAQAQVLHLLEQQMASMPVAVNSERMRAFQAKLVTVMMDESGWSKMEPEYVRL
                     YQDTFNQTEIDGMLAFYRSEAGQAVVAKTPILTRKSMELVMAEQMRLLPRVEALITAF
                     LKEARAAASPKSGKAHH"
     gene            complement(6932..8119)
                     /gene="prpF"
                     /locus_tag="PKN55_07435"
     CDS             complement(6932..8119)
                     /gene="prpF"
                     /locus_tag="PKN55_07435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003294477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylaconitate cis-trans isomerase PrpF"
                     /protein_id="HNX71112.1"
                     /translation="MAHLPQVKIPATYIRGGTSKGVFFRLQDLPEACQVPGAARDQLF
                     MRVIGSPDPYAAHIDGMGGATSSTSKCVILSKSSVPEHDVDYLYGQVSIDKPFVDWSG
                     NCGNLSTAAGAFAIHAGLVDPARIPDNGVAVVRIWQANIQKTIIAHVPVSNGQVQETG
                     DFELDGVTFPAAEIVLEFMDPSDDGEDGGSMFPTGNLVDDLEVPGVGTFKATMITAGI
                     PTVFVNAADIGYTGTELREQINSDPEALKRFEAIRVAGALRMGLIKTPEEAATRQHTP
                     KIAFVAPPTTYTASSGKTIDAGDVDLLVRALSMGKLHHAMMGTAAVAIGTAAAIPGTL
                     VNLAAGGGEREAVRFGHPSGTLRVGAQAKQVDGQWTVTKAIMSRSARIIMEGWVRVPG
                     GTF"
     gene            complement(8179..>8523)
                     /locus_tag="PKN55_07440"
     CDS             complement(8179..>8523)
                     /locus_tag="PKN55_07440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe/S-dependent 2-methylisocitrate dehydratase
                     AcnD"
                     /protein_id="HNX71113.1"
                     /translation="DWAAKGVRLAGVEVIVAEGFERIHRTNLIGMGVLPVEFKPGVTR
                     LTLGLDGTETYDIQGELSPRCDLTLVIHRKNGETTEAQVTCRLDTAAEVRVYQAGGVL
                     QRFAQDFLESNA"
BASE COUNT         1580 a         2645 c         2639 g         1659 t
ORIGIN      
        1 gtctggcggg gttgccccat caccgtgttg ttctgaacaa gagtaaatat gtttaccgat
       61 gtggtgtctg tgtcctgacg gctgaccttg cagggcatgg ccttgttgct gcgggatgtg
      121 atggcggcca taggggtcaa gtcggtggcg gcaggcggag cgtgaggcat gggcggcaac
      181 ggtgtacaat cgccgctcac cgcaaacttg cgcacaaggt gtggccgtgg aaagtctgta
      241 tttgctgatt ccgttttcga ttctgctcgc ctttgtgatt ggcgggctgt tctggtgggc
      301 cacccgctca ggccagttcg acgatctgga aggccccgga catcgcatcc tgatggacga
      361 tgattcgacc aaaccagaca acaaacagcc gccgccctga gcaccgtgct ctgcggcctg
      421 ccgcgccaga gagtttttca tgtccagcac atttgctttt tttgccagcc ggcgttttct
      481 gccgttgttc tctacccagt ttctgggcgc tttcaatgac aatttgctga aaacggcatt
      541 cctggtgtgg gtgagctacg ccgggctgtc atttgccggc ttacctgccg cgcaaatcgt
      601 caatctggct tccggcttgt tcattctgcc gtttttcctg ttttcggcca ccgccggccg
      661 gttggcggaa aaaatcgaca aggcccgtgt ggcccgttgg gtgaagctta gcgagctggc
      721 catcatgttg ctggcggcac tggggttttc gcagcatcac gcgggcgtgc tgctgctggc
      781 gctgttcctg atgggcgccc attccacttt ttttggtccg gtcaaatacg ccatgctgcc
      841 gcagcatctg cgcccgcacg agttgattgg tggcaatggc ctgatcgaga tgggcacttt
      901 tattgccatc ctgcttggcc agatggccgg tggcatgctg gcgctgcatt cactgccact
      961 gaccatcgcg ctgctgatgc tggcggcggc gctgggctgg gccagcagcc ggacaattcc
     1021 gcctgccccg cccagcgcac cggacttgcc gttgcacctg aatttatggc gagacaccgc
     1081 tgatctgcta tgccaggcac gacgcacacc ggccttgtgg accgccatgc tgggaatttc
     1141 ctggttctgg ttgctcggcg cggtatacct gactcaactg cccaccttca cccgcctgca
     1201 cctgggcggc gacccacagg tatacacctt gattctggcg gtgttttcgg tggcgattgg
     1261 cctgggctcg atgctgtgcg cacggctgtc ccatgcccag ctacggctgg gctgggtggg
     1321 cgtgggcttg ctgggcatga cggtggccgg gctggatttg tatgccgcca gtcatgcccg
     1381 gcacagcggc gagctgatgg ggctggcgac atatctggcg cgcccggcgg cttggcgaca
     1441 attgctggat tttgccttgc tgggcggttt tggcggcttg ttcacggtgc cgctgtacac
     1501 ctggctgcaa accgccagcc cggaagattt tcgcgcgcag gcgattgccg ccaacaatat
     1561 cgtcaacggc ctgttcatgg tggcggccgc tgcactcagc gccgtcctgc tggcctggct
     1621 ggacagcatt gccttgctgt ttctctgtgt ggcggtgtgc aatctgcctg ccttgtggtg
     1681 gctggcgcgg cgcgagccga tgctgtggcc gtggcgacgg cagggctaaa agcgtgttca
     1741 aagcgtcgct cgcaacaccc agcgcaaaga cagcaacaag catgtctcag cgcggctgag
     1801 caccagggaa gctttcgaca aactgcacgc gcacatcggg aaagctgtct acatactgga
     1861 tgcgcacatc cggaaaactt tctacctgct gccagcgccc gcagcgatcc ggaaagctat
     1921 tgacccactg cacgcgcata tccggaaacg atgccacgac ttgcacgcgc acatcgggaa
     1981 aactgttcac cacctgcacg cggccggcca ggggcttgcc gttgaatgtg cagcgagccg
     2041 catccaggcc gcccccttgg gccagtgcgc tggcactggc cagtccgatg gccagttggc
     2101 caacacgaag acaagacatg gtatttcctt tctgagtgag tcgggcaaac cctcaatatg
     2161 gccatacgga ttcgcctgag cgcgctggcc caggtcaaca gcgcgccctt gccgtcgtgg
     2221 gttttgcgcg ctatggtaag gcgattgtct gccccttatc caggagcctg ccatgcctgt
     2281 taccccattg gatcgcgcca gtgccgcccg tgtggcggcg ctgccttcac aagcggtgtc
     2341 cagccagcca tttgctgaat tgctacacag caaacaaacc cgcatcggct tgaacccggc
     2401 cagcgtactg gatgccttgt cgcccgcagc ccggcgtgct tttgatgccg ccatgcgtgc
     2461 tgcccccaca ctggcgggcc aggatgtggg ggcgcaagcc gtcagcagtg cgccagcggt
     2521 gctgaatgcc gcactggaca gcctgctgaa tccgagctcg ccagatttca cccccgaggc
     2581 ggtcaagacc gccttgcgcc aattgcaggc cagtctggca gcgggtaaag caccgactac
     2641 gccatctgag acgccgtctg catagctggc tgcggagcca gacccgcgcc ggccatggct
     2701 tcaggtagaa tggcgcattc actgcggccc cgtccgtgtc cgggccgcgc accgttgcca
     2761 ccctgaatct ggagatgccc gatgaaatct tccctgcctg ttgccgtggt gctgtctggt
     2821 tgcggcgtgt atgacggtac tgaaatcacc gaagccgtgg gcctgctgat tgccctgtcg
     2881 caagccggct tcagctaccg ctgttatgcg ccagtgcgcg agcaatatca tgtggtggat
     2941 cacttcaagg gcgcaccggc cgacggcgcg cgcaatatcc tcaccgaatc ggcgcgtatt
     3001 gcccgtggcg ccattcaacc gctgagcgcc ttcaaggctg ccgagcattg cgcgctggcc
     3061 tttcccggcg gttttggcgc cgccaaaaac ctgaccacct ttatcgacca gggcgacaag
     3121 gcgcagctgt acgctgatgt caactcggca gtgaccgacg ccatggcgcg caagctgccg
     3181 ctgctggcgc tgtgcgctgc gccgctggtg ctgggcattg ccgcccgcga cgccggcttg
     3241 agcggcgtgc ggctgacttt tggcgattgc gaaggcccgg ccatgaacgc cgcgctggcc
     3301 acctgggggc agacccatgt ggtcactcca cttgaacagg cctgcgtgga cagcaagcac
     3361 cgcatgatca gtgcaccggc ctatatgtac ggcgacgcca acccggccgg ggtgtttgcc
     3421 agcattcagg cagcggtgaa ggcgctggac gagatgctgg cggccgcttg accgagatcg
     3481 cgtcatcgcc atgacaaagc ggcggacaga gtgaacactg tccgccgctt tgtcacatcg
     3541 tcactggcct gacggcccag ttagcatcag ccagccaggc gcgccagcac ctgttcgcga
     3601 ccaatcaagg ccagcacggc gtccaccgac ggcgtttgcg tggtgccgca caccagcacg
     3661 cgcagcggca tgcccaattg gcccatcttg atgccttctt cggcgcagaa cggcttgaac
     3721 agcccatgga tggcttcggt gttccactcc ggcagcgcag ccagttgggc ggcaaagcgc
     3781 gccatgcgct cgcgggtgtc cggcaggatg tgcttgtcca gctcggcttg ctgcggctgg
     3841 cgcggctgat aaaaataatc gcactcgcgc gccagcgcgt tcagctcctg cacgcgctcc
     3901 ttgaccagcg ccaccacttc agccagcgcc gggccttgtg cggtggagat gcccgccgca
     3961 gtcagccgcg gggtgatcag ctcggccaga cgggcgttgt cggcggcctt gatatgctgg
     4021 gcgttcagcc agaggaattt ttcctgattg aagcggctgg ccgacgggct gaccgcttcc
     4081 aggctgaacc attcgacaaa ctggtccata ttgaaaaact cttcgtcgcc atgcccccag
     4141 cccagccgcg ccaggtagtt gagcagcgcc tcgggcagaa tgcccttgcc ttcgtagtcc
     4201 accacgctga ccgcgtcgcg gcgtttggac atcttgtggc cgtcttcgtt aagaatcatc
     4261 ggcagatggc catacaccgg cagcggcgcg cccagcgctt tcaggatgtt gatctggcgc
     4321 ggggtgttgt tgacgtggtc atcgccacgg atgacgtggg tgatgttcat gtcccagtcg
     4381 tccaccacca cgcagaagtt ataggtgggg ctgccgtccg ggcgggcgat gatcaggtcg
     4441 tcgagttctt cgttctggaa ttcaatccgg cctttgaccg cgtcgtccca ggccacgctg
     4501 ccggacagcg gggttttgaa gcgcaccacc ggcggcacgc cagccggcgg agtgggcagg
     4561 gttttgccgt cttccgggcg ccagcggcgg tcgtagcgcg gtttttcgcc acgggcttcc
     4621 tgctcggcgc gcatggcgtc gagttcttcc ttgctgcagt agcacagata ggcatggccg
     4681 ctggccagca gttgctgaat cacgtcctta tagcggtcaa aacgctgggt ttggtaaaac
     4741 gggccttcgt cgtagtccag gcccacccag tgcatgccgt ccagaatcgc ctgcaccgat
     4801 tcgggagtgg agcgctccag gtcggtatct tcgatgcgca gcacaaaagt gccaccgtgc
     4861 ttacgggcgt aggcccagga gaacagcgcg gtacgaacac cgccaatatg cagaaaaccg
     4921 gtgggactgg gagcaaaacg ggtgcggatc atggaaggct cggccgaaga tagcggaaaa
     4981 atcggcattc tactcttgca ctgccaaggg gaaaaccgct gcacgcggtc aaggcctacg
     5041 ctgttggccc ttgaccggca gcaggagcaa gatgtggcgc tgctcacaaa acacagcgaa
     5101 gcggccctga ccaagcgtat caccgcagac agtacagcga acacgacaag atcacaccac
     5161 aacgccaggg gattttgttc gccgcactca gtgcagcttg acaggggggc taccaatgtc
     5221 agtgaacggc tctatccaca gcagattgcc ttcctgcgca ctgatgatat aagtcgcctc
     5281 gccaaacagg ctggcatgcc ccttggccag ccgggcatcc cagtgtgcgc cacgccactg
     5341 cacgcgcgcc tgctgcgggc tgagccattg ctccaccacc acaggctggc cgatgacttc
     5401 cagcgcctcc agcggccgct gccgttgtat ctgctgatag cgccacagcc gccaacagcc
     5461 cagcccggag agagtcacca ccagcaggcc ctggacattg ccatccagat aaccccacac
     5521 cccacccagc ccgaccagca tcatgctgat gccgatgacc agaaagtaga acgtgcccag
     5581 aatcagctct gccaccaacg caagcgcagc cagaacaaac cacatataag gatggtgcat
     5641 gatggggctc cgtggaaaaa ataggcaaaa gcgatttttg gcaaaccatg ctctcagctt
     5701 ggcaatgcag aacacacggc aatttacgcc aaaagaataa catgggcgca gtataggtag
     5761 tgcggatggt tatgacaatt cgtaggatta cctgcccggc ttgccaaatg ctgcagcgaa
     5821 tgacacctgc catccagcct gcaggccggc ttaagcagtt tcaagcattg aatcggcctg
     5881 gtgcaggcct gtaacatgct gttggcacgc tgtcagtgct tgttctggcc tgacggacat
     5941 gttcaaaacc ccggccgaat ctgtggccag aaagccctgc catgaaaacc tccctgcttg
     6001 ccatcagcct gttgctggtc agccacactt tgcccgtctg ggctagcgag gcgccagcca
     6061 gcgagctatc cgtgcgcgag atgctgcaca tcacccagac cgaacaaaac taccgcgata
     6121 ccatggtgca ggcacaagcc caggttttgc atctactgga acaacaaatg gcatcgatgc
     6181 ccgtagccgt caacagtgag cgcatgcgtg cctttcaggc caaactggtg actgtcatga
     6241 tggacgagtc cggatggagc aagatggaac cagaatacgt ccgtttgtat caggacacat
     6301 tcaaccaaac ggaaatcgac ggcatgctgg cgttttatcg ctctgaggcc ggacaggccg
     6361 tggtggccaa gacaccgatc ctgacgcgca agagcatgga attggtcatg gctgagcaaa
     6421 tgcgcctgct gccacgggta gaagcgttga tcacagcatt tctcaaggaa gcacgtgcag
     6481 ctgcctcgcc aaaatctggc aaggcgcatc attgagatgt ctgattggtt aagcgctgat
     6541 cacaaaaccc cgcacagcgc tcttggatgt gcgatttgtt ggccagcacg gtagtggtat
     6601 tgcgtcgagg cggggccggc ggcgacttca ggctgaacga ttaaaaaaaa gcccggcgca
     6661 aggccgggct caatcacctg ggttttacaa cagacacgaa gaaaccctgt ttgggcggag
     6721 ctgacgatct gcggtcagcc ccgcgcagtg cgtaaaatct ttaactgctc accacactca
     6781 gcgccacaac gccaagaggg cattagtaag cggcgcttag agtggctcac aaaccccagc
     6841 gcagcggtcc tggctaggcg cacgaccgca gacagtacag gtagtacggc aaggtcgtgc
     6901 aacaacgcca ggagggtttt gtgagccgct ctcagaacgt gccgccgggc acgcgcaccc
     6961 aaccttccat aatgatgcgg gcgctgcggc tcatgatcgc cttggtcacc gtccactggc
     7021 cgtccacctg cttggcttgg gcgcccacgc gcaaggtgcc ggacgggtgg ccaaagcgca
     7081 cagcttcgcg ctcgccgccg ccggcggcca ggttgaccag ggtgccgggg atggcggccg
     7141 ccgtgccgat ggccaccgca gcggtgccca tcatggcgtg gtgcagcttg cccatcgaca
     7201 gcgcgcgcac cagcaaatcg acatcacccg cgtcgatggt cttgccgctg gacgccgtgt
     7261 aagtggtcgg cggggcgaca aaggcaatct tcggggtgtg ctggcgggtg gcggcttctt
     7321 ccggggtttt gatcagcccc atgcgcagcg cgccggccac gcgaatcgcc tcaaagcgct
     7381 tgagcgcttc cgggtcgctg ttgatctgct cgcgcagttc agtgccggtg tagccgatgt
     7441 cggcggcgtt gacgaacacc gtcgggatgc cggcagtaat catggtggcc ttgaaggtgc
     7501 ccacgccggg gacttccaga tcgtccacca gattgccggt ggggaacatc gaaccgccgt
     7561 cttcgccgtc gtcggacggg tccatgaact ccagcacgat ttccgccgcc gggaaggtca
     7621 ccccgtccag ctcgaaatcg ccggtttcct gcacctggcc gttgctcacc ggcacgtggg
     7681 cgatgatggt tttctggata ttggcttgcc agatgcgcac cacggccacg ccgttgtcgg
     7741 ggatgcgcgc cgggtccacc aggccggcgt gaatggcaaa cgcgccagcg gcggtggaca
     7801 gattgccgca gttgcccgac caatcgacaa agggcttgtc gatcgacacc tggccataca
     7861 gataatccac atcgtgctcg ggcacgctgg acttggacaa aatcacgcac ttgctggtgc
     7921 tggaggtagc gccgcccatg ccgtcgatgt gcgcggcgta ggggtcgggg ctgccaatca
     7981 cgcgcatgaa cagctggtcg cgcgccgcgc ccggaacctg gcaggcttcg ggcagatcct
     8041 gcaagcggaa aaacacgccc ttgctggtgc cgccacggat ataggtggcg gggattttga
     8101 cttgggggag atgggccatc aaatcagttc cttgcgtcgg gccgcgcccg ccagtccccc
     8161 ggcgggcgcg gcgtcagatc aggcgttgga ttccaggaag tcctgggcaa agcgttgcag
     8221 cacgccgccg gcctggtaca cgcgcacttc ggcggcggtg tccagacggc aggtgacctg
     8281 ggcttcggtg gtttcgccat tcttgcgatg gatcaccagc gtcaggtcgc agcgcggcga
     8341 cagctcgccc tggatgtcgt aggtttccgt gccgtccagg cccagggtca ggcgggtgac
     8401 gcccggcttg aactccaccg gcagcacgcc catgccgatc aggttggtgc ggtgaatgcg
     8461 ctcgaagcct tcggcgacaa tcacttccac ccctgccaga cgcacgccct tggccgccca
     8521 gtc
//
LOCUS       DAOPYX010000118         8471 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_9992_length_8471_cov_7.087928, whole genome shotgun sequence.
ACCESSION   DAOPYX010000118 DAOPYX010000000
VERSION     DAOPYX010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 8471)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8471)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8471
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9992_length_8471_cov_7.087928"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(99..2681)
                     /locus_tag="PKN55_07445"
     CDS             complement(99..2681)
                     /locus_tag="PKN55_07445"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014086892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HNX71114.1"
                     /translation="MNTPLSAEQLANWREKLKQGREKLAEHFARQRDPRWVMKRATDM
                     VDVIIREVWQALALGERAAAIAVGGYGRAQQFPHSDIDLLVLLPENPDQALSAAVEQL
                     IGALWDVGMEVGHSVRTLSECMAEAAGDITIETTLLENRWLIGNAQLHTALNQQLEAH
                     IDPVAFFEAKQFEQQQRHNKYFGVANNLEPNVKESPGGLRDLQTILWISKASGLGDSW
                     GALVEGGILTAQEARLIRHSEHQLQCLRIDLHLTAKRREDRLIFDLQQQVALRWGLQD
                     SPSRRASEQLMQLYYRAARLVSQLNGILLPNLKSRLYSQVRQVSHVLNERFRTLNGML
                     AVRRPDVFEKTPSAILEGFLLLQRHPELTGFTPKTLRALWHARGKINGAFRRDPANQA
                     LFLQMFREPAGLTRGLRRMNLYGVLGRYLPAWGRIVGQMQHDLFHVYTVDEHILMVVR
                     NLRRFASPAFNHEYPFCSKLLNDFERPEALYLAGLFHDIAKGRGGDHSKLGMADAREF
                     CQTHGLPPEDIDLVEWLVGEHLTMSAVAQKEDIYDPETVQAFAERVGDVRRLTALYLL
                     SVADIRGTSPKVWNAWKGKLLEDLYTSTLRMLARGGQVDTESELEERRNEARNLLRLS
                     LVPDGVEEKLWKQLDTVYFLRHEAREIAWHARVLNRFVDTDAPIVKARISTSKEGIKV
                     LVYTPDQPDLFARICGFFGRTHYSIADAKIYTTRHGYALDTFHVFVPEHFDGDYRDLI
                     NYVEFELAALLTRQAPLAPPSGGRINRHLKYFPIEPQVSIRPDDRGNYHVLSIVTGDR
                     PGLLARIAWVLSRHQVSVQSAKIMTLGSRVEDSFLLQGGALNDAKRALQLEGELLDEL
                     QLQG"
     gene            complement(2712..3785)
                     /gene="lptG"
                     /locus_tag="PKN55_07450"
     CDS             complement(2712..3785)
                     /gene="lptG"
                     /locus_tag="PKN55_07450"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008953921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HNX71115.1"
                     /translation="MSLLTRYLISRISLVTLFTLLALVALFSFFDIINEAGDLGKGSY
                     TLGIMLVHVLLQIPGHIYELLPLAVLIGTLVAMSLLASGSEYTVMRASGVSLLRVGSI
                     LVQIGLVFALVTLLMGEFIAPKAEQEAERLKIRATRSVVAQQFNSGSWMKDDRNFINV
                     REILPDNSLIGLRIYSYDADLRLTRTRYAERGFYLGDGSWRLEKVQDSDITPERIVRS
                     HHDALVWKSVIRPEILSVLLVVPEQMSGLNLMAYIDHLKNNSQKTSRYEIALWGKIFY
                     PLACLSMALMALAFTPVNRREGNLGMQLFIGILIGLSFHFFNRLLGHLGLLYDWWPAA
                     VAIVPTLTFLAAGAVLTARRERR"
     gene            complement(3787..4872)
                     /gene="lptF"
                     /locus_tag="PKN55_07455"
     CDS             complement(3787..4872)
                     /gene="lptF"
                     /locus_tag="PKN55_07455"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012696889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HNX71116.1"
                     /translation="MIFQRSLIRELTQVAMAVFTVLLAIVLTTQVLKLFGRAAAGQLA
                     SDAIFAMVGFATLGSFGVVLSLTLFISIMIVLTRLHRDSEMAVWQTSGLSPTAWISPV
                     LKFAVPLVALVAVVSLFLAPWAKGKGNQYANLLKQREEISALAPGVFKETQGGKRVYF
                     IENYSGEAGAANNIFVQLIDSEGRLSSVFAQSGRLYTDDTHGERFLLLEHGRRYEGEA
                     GRQDFRVVEFQRYRVRIDQGLKPLSESSSKIRPTVELMTEGDQGSRGELAWRFSVPIS
                     ALILALLAIPLSHYNPRAGQTLNLLVALFIYMFYYNTMGVVESAISRGKVGWLTGMLP
                     LHLSLFILFVGLMAWRSGALRRNKGVC"
     gene            5078..6646
                     /locus_tag="PKN55_07460"
     CDS             5078..6646
                     /locus_tag="PKN55_07460"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008953923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HNX71117.1"
                     /translation="MEFNIKSGSPEKQRVACVIVGVYEARKLSFAADLLDRISNGFIS
                     DVLRRGDMEGKLGSTLVLHSVPHTLCDRVMLVGLGKERDFREKEYRDAVRASLAALAK
                     TAAVEAVSYLTELTLKKRDVEWMIETAACLSLDAQYRFDQFKSKTDDAPRSLRKLTLS
                     VSRRSDLAEGEVGLVRGLAIANGMKLARDLGNLPGNVCTPAYLADTARALAERFGCEC
                     TVLEQDDMAELGMGSLLSVGKGSIQPPKLIVLKHNGGPAGQKPVVLVGKGITFDTGGI
                     SLKPGEGMDEMKFDMCGAASVLGAFQAAVEMKLPLNVITVVAAAENMPSGGASKPGDI
                     VTSLSGQTIEILNTDAEGRLVLCDALTYCERFEPAAVVDVATLTGACIIALGHQASAL
                     FSNQDHLSRELQAAADEAADRVWPMPMWEEYHEVLKSNFADVANVGGRPGGSITAACF
                     LSRFTKAYDWAHLDIAGVAWKSGKEKGATGRPVPLLTQFLQDRADTAAGNVVRRGRPR
                     RDAADTSHDDDAAD"
     gene            6630..7058
                     /locus_tag="PKN55_07465"
     CDS             6630..7058
                     /locus_tag="PKN55_07465"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014086896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HNX71118.1"
                     /translation="MTQRIDFYHHVRDPLAFACKLAATVMGKGQRLLVLLADPAQLHA
                     FDERLWTWQPTSFIPHCRLDEPIAADTPVLLATRLPPGPAPCPVLLNLSLGVPDEYSR
                     FERLLEIVGEDPDSLARARQVARAYKAAGLETVYHDMTGR"
     gene            7207..>8471
                     /locus_tag="PKN55_07470"
     CDS             7207..>8471
                     /locus_tag="PKN55_07470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012696595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HNX71119.1"
                     /translation="MELAKSFEPGDLENHWYARWESAGYFKPHMDLSKPSFCIQLPPP
                     NVTGTLHMGHAFNQTIMDGLTRYHRMKGDNTSWIPGTDHAGIATQIVVERQLAEQGVS
                     RHDLGREDFISKVWEWKAKSGGTITSQMRRVGCSVDWEREYFTMDATRAEVVTEVFVR
                     LFEQGLIYRGKRLVNWDPKLGTAVSDLEVISEEEDGHMWHIRYPVVGSDDTVIVATTR
                     PETLLGDVAVAVNPNDERYQHLVGKQLQLPLTGRQIPVIADDYVDAAFGTGFVKITPA
                     HDFNDYQVGKRHATQLINVMSLEAKILAKAQVFGFDGSVQGQIDLPAAYAGLSTAEAR
                     KAMVADLDAAGLLVEVKPHKLMTPRGDRTGSVIEPLLTDQWFVAMSKVSEQDPTGKSI
                     TQKAIDAVESGQVRFIPENWVNTYNQWMN"
BASE COUNT         1713 a         2683 c         2587 g         1488 t
ORIGIN      
        1 aaaaacccag gattgccgct ggtgcggaca tcctgggttt tgattttttg gcaaatacga
       61 tcaatcttgc ggcttgcggg atcaatccac gccgccctct aaccctgcaa ttgcagttcg
      121 tccagcagtt cgccttccag ttgcagcgcg cgtttggcgt cgttcagcgc gccgccttgc
      181 agcaggaagg agtcttccac ccgtgagccc agggtcatga ttttggcgga ttgcacgctc
      241 acctggtggc gcgacagcac ccaggcgatg cgcgccagca ggccggggcg gtcgccggtg
      301 acgatggaca gcacatggta gttgccgcga tcgtccgggc ggatgctgac ctgtggttcg
      361 atggggaagt atttcaaatg gcggttgatg cgcccgccgc tgggcggcgc cagcggggcc
      421 tggcgggtga gcagggcggc cagttcaaat tccacatagt tgatcaggtc gcggtagtcg
      481 ccgtcaaagt gttcgggcac aaacacatga aaggtgtcca gcgcgtagcc gtggcgggtg
      541 gtgtaaatct tggcgtcggc aatgctgtag tgggtgcggc caaaaaagcc gcagatgcgg
      601 gcaaacaggt cgggctggtc gggggtgtag accagcacct tgatgccttc tttgctggtg
      661 gaaatccgcg ccttgacgat gggggcgtcg gtgtcgacaa agcggttgag cacgcgcgcg
      721 tgccaggcga tttcccgcgc ttcgtggcgc agaaaataca ccgtgtccag ctgcttccac
      781 agtttttctt cgacgccgtc gggcaccagc gacaggcgca gcaggttgcg cgcttcgttg
      841 cggcgttctt ccagctcgga ttcggtgtcc acctggccgc cgcgcgccag catgcgcagg
      901 gtggaggtgt acaggtcttc cagcagcttg cctttccagg cgttccacac cttggggctg
      961 gtgccgcgaa tgtccgccac cgacaagaga tacagcgcgg tcagccggcg aacgtcgccc
     1021 acccgctcgg caaaggcttg cacggtttcc gggtcgtaga tgtcttcttt ttgcgccacc
     1081 gccgacatgg tgagatgctc gcccaccagc cattccacca gatcaatgtc ttctggcggc
     1141 aggccgtggg tctggcaaaa ttcgcgggcg tcggccatgc ccagcttgga atgatcgcca
     1201 ccccggcctt tggcgatgtc gtggaacaag ccagccagat acagcgcttc cgggcgctca
     1261 aagtcgttca gcagcttgct gcaaaacggg tattcgtggt tgaaggccgg cgaggcaaag
     1321 cggcgcagat tgcgcaccac catcaggatg tgttcgtcca cggtgtagac atgaaacaaa
     1381 tcatgctgca tctggccgac aatgcgcccc caggccggca ggtagcggcc aagcacgccg
     1441 tataaattca tccgccgcag gccgcgcgtc agcccggccg gctcacgaaa catttgcaaa
     1501 aacagcgcct gattggccgg gtcgcggcga aaagcgccgt tgattttgcc gcgcgcgtgc
     1561 cacagggcgc gcagggtttt tggggtaaag ccggtcagct cggggtggcg ctgcaacagc
     1621 aaaaagcctt ccaggatggc ggacggggtt ttttcaaaca catcggggcg gcgcaccgcc
     1681 agcatgccgt tcagcgtgcg aaaacgctcg ttcagcacat ggctgacctg gcgcacttgc
     1741 gaatacagcc gggatttcag gttgggcagc aggatgccgt tcagttggct gaccagccgg
     1801 gcagcgcggt aatacagctg catcagctgc tcggaggcgc gccggctggg gctgtcttgc
     1861 aggccccagc gcagggcgac ttgctgttgc aggtcaaaaa tcagccggtc ttcgcgccgt
     1921 ttggcggtca ggtgcaggtc gatgcgcagg cattgcagtt ggtgttcgct gtggcgaatc
     1981 agccgcgctt cctgggcggt gaggatgccg ccttccacca gcgcgcccca gctgtcgccc
     2041 aggccgctgg ccttgctgat ccacaaaatg gtttgcaggt cgcgcaggcc gccggggctt
     2101 tctttgacgt tgggctccag attattggcc acgccaaagt atttattgtg ccgctgctgt
     2161 tgctcgaatt gcttggcctc aaaaaacgcc accgggtcga tgtgcgcttc cagctgttgg
     2221 ttcagcgcgg tgtgcagctg ggcgttgcca atcagccagc ggttttccag cagggtggtt
     2281 tcgatggtga tgtcgccagc ggcctcggcc atgcattcgc tcagcgtgcg cacgctatgg
     2341 ccgacttcca tgcccacgtc ccacagcgcg ccaatcagct gttccaccgc cgccgacagg
     2401 gcctggtcag ggttttctgg cagcagcacc aaaagatcaa tgtccgagtg cggaaactgc
     2461 tgggcgcggc catagccgcc cacggcaatt gccgccgcgc gctcgcccag cgccagcgcc
     2521 tgccagactt cacggatgat cacgtccacc atatcggtgg cgcgcttcat cacccagcgc
     2581 ggatcacgct ggcgggcaaa atgttcggcc agtttttcgc ggccttgctt gagtttttcg
     2641 cgccagttgg caagctgctc agcggacagg ggggtattca tccagcaacg cttccggggg
     2701 taggatcagg gtcagcgacg ttcgcgccgc gcagtcagga cggcgccagc agccaaaaag
     2761 gtcagcgtag gcacaatcgc caccgccgcc ggccaccagt cgtacagcaa gcccagatgg
     2821 cccagcaggc ggttgaaaaa gtgaaacgac aggccaatca ggatgccgat gaacagctgc
     2881 atgcccagat tgccttcgcg gcgattgacc ggggtgaagg ccagcgccat cagcgccatc
     2941 gacaaacagg ccagcgggta aaaaatcttg ccccacagcg cgatttcata gcgcgaggtt
     3001 ttctggctgt tgtttttcag gtggtcgata taggccatca ggttcaggcc ggacatctgc
     3061 tcgggcacca ccagcagcac cgagaggatt tccgggcgaa tgaccgactt ccacaccagg
     3121 gcatcatggt ggctgcgcac gatgcgctcg ggcgtgatgt cggaatcctg gactttttcc
     3181 agccgccagc tgccgtcacc cagataaaaa ccacgctcgg cgtagcgggt gcgggtcagg
     3241 cgcagatcag cgtcgtagct gtaaatacga aggccaatca aggaattatc cggcagaatt
     3301 tcccgcacat taataaaatt gcggtcgtct ttcatccacg agccagagtt aaactgctgg
     3361 gccaccaccg agcgggtggc gcggattttc agccgctcgg cctcttgctc ggccttgggg
     3421 gcaataaatt cgcccatcag cagcgtaacc agggcaaaca ccaggccgat ttgcaccagg
     3481 atgctgccca cgcgcagcaa agacaccccc gaggcgcgca tcaccgtgta ttccgagccc
     3541 gaagccagca ggctcatcgc caccagggta ccaatcagca ccgccagcgg cagcagctcg
     3601 taaatatggc cgggaatttg cagcagcaca tgcaccagca tgatgcccag ggtatagctg
     3661 cctttgccca ggtcgccagc ttcgttgatg atgtcaaaaa acgaaaacaa cgccaccagc
     3721 gccagcaggg tgaacaaggt caccaggctg atgcgcgaaa tcagataacg ggtgagcaaa
     3781 ctcatctcag cacaccccct tgttgcgccg cagggcgcca ctgcgccagg ccatcagacc
     3841 gacaaacagg ataaacagcg ataagtgcaa aggcagcatg ccggtcagcc agcctacctt
     3901 gccgcgcgag atggctgatt cgaccacgcc catggtgttg taataaaaca tgtaaatgaa
     3961 cagcgccacc agcaggttga gggtttgccc ggcacgcggg ttgtaatgcg acagcggaat
     4021 ggccagcaag gccaaaatca aggcgctgat cggcactgag aagcgccagg ccagctcgcc
     4081 acggctgccc tgatccccct cagtcatcag ctcgaccgtg ggacgaatct tgctgctgct
     4141 ttcgctcagc ggcttcaagc cctggtcaat gcgcacccgg taacgctgaa actccaccac
     4201 ccggaaatcc tgacggccgg cctcgccctc gtagcggcgg ccatgctcca gcagcaaaaa
     4261 gcgctcgcca tgggtgtcgt cggtatacag ccggcccgat tgggcaaaca ccgacgacaa
     4321 gcggccctcg ctgtcgatca actgcacaaa gatattattg gcagcgccgg cctcgcccga
     4381 gtaattctcg ataaagtaca cccgcttgcc gccctgggtt tctttgaaca cccccggcgc
     4441 cagcgccgag atttcttcgc gctgcttaag gaggttggca tactgattgc ctttcccctt
     4501 ggcccacggc gccagaaaca ggctgaccac cgccaccagc gccaccagcg gcacggcaaa
     4561 tttcagcacc gggctgatcc acgcggtcgg cgacaagccg gaggtttgcc acacggccat
     4621 ttcgctgtcg cgatgcaggc gggtgagcac aatcatgatg gagataaaca aggtcagcga
     4681 cagcaccacg ccaaacgacc ccagcgtggc aaagcccacc atggcaaaga tggcgtcaga
     4741 agccagctgg ccagcggcgg cgcggccaaa cagcttgagc acctgggtgg tcagcacaat
     4801 ggcgagcagc acggtaaaca ccgccatcgc cacctgagtg agctcacgaa tcaggctgcg
     4861 ttgaaaaatc atgcgaaaag cgccttcttt gactttttat cagggacagg ccaataattg
     4921 aaaacaatgc aaaaacggcg gtgatggtga attttctgcg ctttggcgcg gttttcacca
     4981 gacttcgacg cgtcatgggc gcggccgttc ctgatgtctg cacatattcc cggccgggcc
     5041 gaccggcctg caaccctgcc aaatttggga gcaaaccatg gaatttaaca taaaaagcgg
     5101 cagccctgaa aaacagcgcg tggcctgcgt gattgtcggc gtctacgaag cccgcaaact
     5161 gtcgtttgcc gctgacctgc tggatcgcat ctccaacggc tttatcagcg acgtgctgcg
     5221 tcgcggcgat atggaaggca agctgggcag cacgctggtg ctgcattcgg tgccgcacac
     5281 cctgtgcgac cgggtgatgc tggtcggcct gggcaaagag cgcgatttcc gcgaaaaaga
     5341 ataccgcgac gccgtgcgcg cctcgctggc ggcgctggcg aaaaccgccg ccgtcgaggc
     5401 agtgagctac ctcaccgaac tcaccctgaa aaaacgcgat gtcgaatgga tgatcgaaac
     5461 cgccgcctgc ctgtcgctgg acgcgcagta tcgttttgat cagttcaaat ccaaaaccga
     5521 cgacgccccg cgcagcctgc gcaagctcac cctgtcggtc agccgccgca gcgacctggc
     5581 cgaaggcgaa gtgggcctgg tgcgcggcct ggccattgcc aacggcatga aactggcccg
     5641 cgacctgggc aatctgccgg gcaatgtctg caccccggcc tatctggccg acaccgcccg
     5701 cgcgctggcc gagcgctttg gctgcgaatg cacggtgctg gagcaggatg acatggccga
     5761 gctgggcatg ggctcgctgc tgtcggtggg caagggcagc atccagccgc ccaagctgat
     5821 tgtcctcaag cacaacggcg gcccggccgg gcaaaagccg gtggtgctgg tgggcaaggg
     5881 catcaccttt gacaccggtg gcatttcgct caaaccgggc gaaggcatgg acgaaatgaa
     5941 gttcgacatg tgcggcgccg ccagcgtgct gggcgccttc caggcggcgg tggaaatgaa
     6001 gctgccgctg aacgtcatca ccgtggtggc cgccgctgag aacatgccgt ccggcggcgc
     6061 ctccaagcca ggcgatatcg tcaccagcct gtctggccag accatcgaaa tcctcaacac
     6121 cgacgccgaa ggccggctgg tgctgtgcga tgcgctgacc tactgcgagc gcttcgagcc
     6181 cgccgccgtg gtggacgtgg ccacgctgac cggcgcgtgc atcatcgccc tgggccatca
     6241 ggccagcgcg ctgttcagca atcaggatca cctcagccgc gagctgcaag ccgccgccga
     6301 cgaagctgcc gaccgcgtgt ggccgatgcc gatgtgggag gaataccacg aggtgctgaa
     6361 aagcaacttc gccgacgtgg ccaatgtcgg cggccgcccc ggcggcagca tcaccgccgc
     6421 gtgctttttg tcgcgcttca ccaaggctta cgactgggcg cacctggaca tcgccggcgt
     6481 ggcgtggaag agcggcaaag aaaaaggcgc caccggccgc ccggtgccgc tgctgaccca
     6541 gttcttgcag gaccgcgccg acaccgccgc cggcaatgtg gtgcgccgtg gccgtccgcg
     6601 ccgcgacgcc gccgacacga gccacgacga tgacgcagcg gattgacttt tatcaccacg
     6661 tgcgcgaccc gctggccttt gcctgcaagc tggccgccac ggtgatgggc aagggtcagc
     6721 gcttgctggt gctgctggcc gaccccgccc agctgcacgc gtttgacgaa cggctgtgga
     6781 cctggcagcc caccagtttt atcccgcatt gccggctgga cgagcccatc gccgccgaca
     6841 ccccggtgct gctggccacc cggctgccgc cggggccggc gccgtgccca gtgctactga
     6901 atctgtcgct gggcgtgccg gacgagtatt cccgctttga acgcctgctg gaaatcgttg
     6961 gcgaagaccc cgactcactc gcccgcgctc gccaggtggc ccgagcctac aaggccgccg
     7021 ggttagagac ggtataccac gacatgaccg gccgctgatg cggccggttt ttttgtgccc
     7081 atccgccggc cgggcccatt ctgccctgag ccggctttct cttccgccgc gcgcccaccg
     7141 ccagccaccc cagccgccgg tgtataatca gacacttttc tcatcaaaat tccgcataag
     7201 cacaacatgg aacttgccaa aagcttcgaa ccgggtgatc tggaaaacca ttggtacgcg
     7261 cgctgggaaa gcgccggcta tttcaagccg cacatggacc tcagcaaacc gtccttttgc
     7321 atccagctgc cgccgcccaa tgtgaccggc accctgcaca tgggccacgc cttcaaccag
     7381 accatcatgg acggcctgac ccgctatcac cgcatgaagg gcgacaacac cagctggatt
     7441 cccggcaccg accacgccgg catcgccacc cagattgtgg tggagcgcca actggccgaa
     7501 caaggcgtca gccgccacga cctgggccgc gaagacttca tcagcaaggt gtgggaatgg
     7561 aaagccaaat ccggcggcac catcaccagc cagatgcgcc gcgtgggctg ttcggtagac
     7621 tgggagcgcg aatacttcac catggacgcc acccgcgccg aagtggtcac cgaagtgttt
     7681 gtccgcctgt ttgaacaagg cctgatctac cgaggcaagc gcctggtcaa ctgggacccg
     7741 aaactgggca ccgccgtgtc tgacctggaa gtgatctcgg aagaagaaga cggccatatg
     7801 tggcatatcc gctacccggt ggtgggcagc gatgacacgg tgattgtcgc caccacccgc
     7861 ccggaaaccc tgctgggcga cgtggcggtg gcggtgaacc ccaatgacga gcgctatcag
     7921 cacctggtgg gcaagcaact gcaactgccg ctgaccggcc gccagattcc ggtgattgcc
     7981 gatgactacg tcgatgccgc ctttggcacc ggctttgtca aaatcacccc ggcgcacgac
     8041 ttcaacgact atcaagtggg caagcgccac gccacccagc tgatcaatgt gatgagcctg
     8101 gaagccaaaa tcctggccaa ggcgcaggtg tttggctttg acggcagcgt gcaaggccaa
     8161 atcgacctgc ccgccgccta cgccggcctg agcaccgccg aagcgcgcaa ggccatggtg
     8221 gccgatctgg acgccgccgg cctgctggtg gaagtcaaac cgcacaaact gatgaccccg
     8281 cgcggcgacc gcaccggctc ggtgatcgaa cccctgctga ccgaccaatg gtttgtcgcc
     8341 atgagcaagg tcagcgaaca agaccccacc ggcaaatcca tcacccaaaa agccatcgac
     8401 gcggtggaat ccggccaggt gcgctttatc ccggaaaact gggtgaacac ctacaaccag
     8461 tggatgaaca a
//
LOCUS       DAOPYX010000119         8461 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_10011_length_8461_cov_11.463597, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000119 DAOPYX010000000
VERSION     DAOPYX010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 8461)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8461)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8461
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10011_length_8461_cov_11.463597"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(211..2505)
                     /gene="clpA"
                     /locus_tag="PKN55_07475"
     CDS             complement(211..2505)
                     /gene="clpA"
                     /locus_tag="PKN55_07475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0043335 - protein unfolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HNX71120.1"
                     /translation="MIAQELEVSLHMAFMDARQKRHEFISVEHLLLAMLDNPSAADVL
                     RACGANLDQLRKNLSEFIEQHTPLVSGTDEVETQPTLGFQRVIQRAILHVQSSGKKEV
                     TGANILVAIFGEKDSHAVYYLHQQNISRLDVVNYISHGISKQKSSEQPPRSSGEESSD
                     EQETAGHGGALENYTQNLNQQATAGKIDPLIGRDHELERVIQILCRRRKNNPLLVGEA
                     GVGKTAIAEGLARRIVRGEVPDVLADATVYSLDMGSLLAGTKYRGDFEQRLKSVIKQL
                     TEDKHAILFIDEIHTLIGAGAASGGTLDASNLLKPALSNGSLRCIGATTYNEFRGIFE
                     KDNALSRRFQKIDVAEPTVEQTVEILKGLKSRFEAHHGVKYTAAALTTAAELAARYIN
                     DRHLPDKAIDVIDEAGAAQKILPKSRQKKVINKTEIEDIITKIARIPAKTVSNDDRSA
                     LKTLDRDLKNVVFGQDAAIDALAAAIKMSRSGLGNPAKPIGSFLFSGPTGVGKTEVAK
                     QLAYTLGVELLRFDMSEYMERHAVSRLIGAPPGYVGFEQGGLLTEQVTKHPYAVLLLD
                     EIEKAHPDIFNVLLQVMDHGTLTDNNGRKADFRNVVIIMTTNAGAESLSKSSIGFTSS
                     KASGDEMADIKRLFTPEFRNRLDAIISFKMLDEAIILRVVDKFLMQLEAQLNEKKVEI
                     HFTDALKSHLAKHGFDPQMGARPMARLIQDTIRKALADELLFGRLADGGEVTVDMDEH
                     DKVKLEFAVKESNAKDKGSAPIPA"
     gene            complement(2502..2813)
                     /gene="clpS"
                     /locus_tag="PKN55_07480"
     CDS             complement(2502..2813)
                     /gene="clpS"
                     /locus_tag="PKN55_07480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008953392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HNX71121.1"
                     /translation="MPRRTQDDVALVPMKTRTAPPPLYKVLLLNDDFTPMEFVVQVLQ
                     RIFRMDQEQATRVMLTVHTEGRGVCGVYTKDIAATKVDQVLDMARVHQHPLQCVMEEN
                     G"
     gene            3428..3631
                     /locus_tag="PKN55_07485"
     CDS             3428..3631
                     /locus_tag="PKN55_07485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HNX71122.1"
                     /translation="MATGTVKWFNDAKGFGFITPDDGGEDLFAHFSAINMNGFKTLKE
                     GQRVTFEVTQGPKGKQASNIQSQ"
     gene            3882..4088
                     /locus_tag="PKN55_07490"
     CDS             3882..4088
                     /locus_tag="PKN55_07490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012695856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HNX71123.1"
                     /translation="MATGIVKWFNDAKGFGFITPDDGGEDLFAHFSAINMNGFKTLKE
                     GERVSFDVAQGPKGKQAANIQSAS"
     gene            complement(4185..5429)
                     /gene="icd"
                     /locus_tag="PKN55_07495"
     CDS             complement(4185..5429)
                     /gene="icd"
                     /locus_tag="PKN55_07495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008953390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HNX71124.1"
                     /translation="MTTSHITVPQGGQKIVPGQAIPDNPIIPFIEGDGIGIDITPVMI
                     KVVDAAVSKAYNNSKKIHWMEIYAGEKSTRVYGGDVWLPEETMAALKDYVVSIKGPMT
                     TPVGGGIRSLNVALRQELDLYVCLRPVRYFTGVPSPLKRPDLTDMVIFRENTEDIYAG
                     IEWQAETDGAKKLINFLQTEMGVKKIRFPETSGIGIKPVSKQGTERLVRKAIQYAIDN
                     DRKSVTIVHKGNIMKFTEGGFRDWAYDLAKNEFGAQLVDGGPWCKFTNPKTGKDIIVK
                     DAIADAFLQQILLRPAEYDVIATCNLNGDYISDALAAQVGGIGIAPGANLSDSVAMFE
                     ATHGTAPKYAGLDKVNPGSLILSAEMMLRHMGWTEAADLIISSMEKAIGDKVVTYDFA
                     RLMDGATEVSCSAFGDAMIERM"
     gene            5826..6485
                     /locus_tag="PKN55_07500"
     CDS             5826..6485
                     /locus_tag="PKN55_07500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018748496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HNX71125.1"
                     /translation="MSELILFNKPYGVICQFSAHPKHPSLADYVRIPDVYPAGRLDTD
                     SEGLLLLTADGALQHQISHPGCKLPKTYWAQVEGIPDDDALAQLRAGVDLGDFVTQPA
                     QVRRIDPPSLWPRTPPIRVRQSVPDSWLEIIIHEGKNRQVRRMTAKVGHPTLRLVRAA
                     IGPWSLGDLPPGELRQLTVSAPPLANGGKPAHHDRAMSRADRPAPPRPGVRARHAGRR
                     R"
     gene            6625..8391
                     /gene="aceK"
                     /locus_tag="PKN55_07505"
     CDS             6625..8391
                     /gene="aceK"
                     /locus_tag="PKN55_07505"
                     /EC_number="2.7.11.5"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018150441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008772 - [isocitrate dehydrogenase
                     (NADP+)] kinase activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
                     /protein_id="HNX71126.1"
                     /translation="MNTPSELNAVAWDIAQTLIKGFNKHYFLFRECSRNAKEYFDQGD
                     WHAMQRAITERIQFYTERVMETVEQLQCRFHADALDDSTWQQVKLYFIGMLMNHKQPE
                     LAETFFNSVFCRILDRDYFNSDFIFTRPVISTEYIESDPPIYRSYYPGPKGLCQTLEQ
                     IVADFGWNRPFAHLRRDIVKVMRAAREHLGGQWATPDVNYQIQVLYSGFYRNKSAYIM
                     GKVINGNQSYPFALCIMHNDDGELEIDTVLLEPWRIGVLFSFSRAYFLVDMEVPSGYV
                     QFLRGMLPWKTKAEIYTMLGLQKQGKNTFYRDFMHHLQHSNDDFIIAPGIRGLVMLVF
                     TLPSYPFVFKLIKDVFGSSKEVDRPTVKAKYLLVKRHDRVGRMADTLEYSNVAFPKKR
                     FTEELLTELRTLAPSLLEESDDTLVIKHLYIERRMKPLNIYLDNATPEEKEHAVREYG
                     DAIKDLAQANIFSGDMLFKNFGVTRYGRVIFYDYDEIEYMTDCNFRDIPEPPSPEYEM
                     ASEPWYPVAKNDIFPEEFASFLLGDPLVRKFFIKYHRDLLSPAFWRTQQTNIRAGKMD
                     NFYPYPQALRLCHRYPDKAASK"
BASE COUNT         1635 a         2575 c         2462 g         1789 t
ORIGIN      
        1 acgctgaatg gcgaggggct ggcattgcct ggcccaagaa aatccgccca aaaacaccgc
       61 ctacctgccc tttatggcgg cgatgcgcca tgccttaagc atgaggttcg gcaagccgtc
      121 tattgcgcat aacttcgtgc aagcccatcg atctagccaa gaaaaacccc gccgaagcgg
      181 ggttgccaag tggcgcacgg atgcttgaaa tcaggccgga atcggggcgc tgcctttgtc
      241 tttggcgttg ctttccttga ccgcgaactc cagtttcacc ttgtcgtgct cgtccatgtc
      301 caccgtcact tcgccgccgt cggccaggcg gccgaacagc agttcgtcgg ccagggcttt
      361 gcggatggtg tcctggatca gccgcgccat cggccgcgcg cccatttgcg ggtcgaagcc
      421 gtgtttggcc aggtgcgact tgagcgcgtc ggtgaagtgg atttccacct ttttctcgtt
      481 cagctgtgct tccagctgca tcaggaactt gtccaccacg cgcaggataa tcgcctcgtc
      541 cagcatcttg aacgagatga tggcgtccag ccggttgcgg aattccgggg tgaacaggcg
      601 cttgatgtcg gccatctcgt cgccgctggc cttgctgctg gtaaagccga tgctggactt
      661 ggacaggctt tccgcgccgg cattggtggt catgatgatc accacattgc gaaaatccgc
      721 cttgcggccg ttgttgtcgg tcagcgtgcc gtggtccatc acctgcaaca gcacattgaa
      781 gatgtccggg tgggcttttt cgatttcgtc cagcagcaac accgcgtacg ggtgcttggt
      841 gacttgctcg gtgagcaggc cgccctgttc aaaacccaca tagcccggcg gcgcgccaat
      901 cagccgcgac acggcgtggc gctccatgta ttcggacatg tcaaagcgca gcagctccac
      961 gcccagcgtg taggccagct gcttggccac ttcggtcttg cccaccccgg tcgggccgga
     1021 gaacaggaag gagccaatcg gcttggccgg gttgcccagg ccagagcgcg acatcttgat
     1081 ggcggcggcc agcgcgtcga tggcggcatc ctggccaaac accacgtttt tcaggtcgcg
     1141 gtccagggtc ttgagcgcgc tgcggtcgtc gttggacacc gtcttggccg ggatgcgggc
     1201 aatcttggtg atgatgtcct cgatctcggt cttgttgatc accttcttct gccgcgactt
     1261 gggcaaaatc ttttgcgcag cgccggcttc gtcgatcacg tcaattgcct tgtccggcag
     1321 gtgacggtcg ttgatgtagc gcgcggccag ctcggcagcg gtggtcagcg cggcggcggt
     1381 gtacttgacg ccgtggtggg cctcaaagcg cgatttcagc cccttgagga tttccaccgt
     1441 ctgctcgacc gtcggctcgg ccacgtcgat cttctggaag cggcgcgaca gcgcgttgtc
     1501 tttttcgaag atgccacgga actcgttgta ggtggtggcg ccaatgcagc gcagcgagcc
     1561 attggacaag gccggcttga gcaggttgga agcatccagc gtgccgcccg acgccgcccc
     1621 cgccccgatc agggtgtgga tttcgtcgat gaacaggatg gcgtgcttgt cctcggtcag
     1681 ctgcttgatc acgctcttga gccgttgctc aaagtcgccg cgatacttgg tgccggccag
     1741 cagcgagccc atgtccagcg aatagacggt ggcgtcggcc agcacgtccg gcacttcgcc
     1801 gcgcacgatg cgccgcgcca ggccttcggc aatggcggtt ttgcccacac cggcctcgcc
     1861 gaccagcaac gggttgtttt tgcgccgacg gcagaggatc tggatcaccc gctccagctc
     1921 gtggtcgcgg ccgatcagcg gatcgatctt gccggcggtg gcttgctggt tgaggttttg
     1981 cgtgtagttc tccagcgcgc caccatggcc ggcggtttcc tgctcgtcgc tgctctcctc
     2041 gcccgagctg cgcggcggct gctcgctgct cttttgcttg gagatgccgt gcgagatgta
     2101 gttgaccaca tccaggcggc tgatgttctg ctggtgcagg tagtacaccg cgtgcgagtc
     2161 cttttcgcca aagatggcaa ccaggatatt cgcgccggtg acttcttttt tgccggacga
     2221 ttgcacatgc aggatggcgc gctggatcac gcgctgaaag cccagcgtgg gctgggtttc
     2281 cacttcatcg gtgcccgata ccagcggcgt gtgctgttcg atgaattcgg acaggttctt
     2341 gcgcaactga tccaggttgg cgccgcaggc gcgcaacaca tcggcggcag aggggttgtc
     2401 gagcatggcc agcaacaggt gttcaacgct gatgaactca tggcgttttt gacgtgcgtc
     2461 catgaaggcc atgtgcaggc tcacttcaag ttcttgggcg atcatccgtt ttcctccatt
     2521 acgcactgca aggggtgctg gtgaaccctg gccatatcga gcacctgatc caccttggtg
     2581 gcagcgatgt ctttggtgta aaccccacag acgccgcgcc cttcggtgtg gacggtgagc
     2641 atgactcggg ttgcttgttc ctgatccatc cggaagatgc gttgcaaaac ttgcaccaca
     2701 aactccatcg gcgtgaaatc atcgttcaaa agtaaaactt tgtacaaagg cggcggcgcc
     2761 gtccgtgttt tcatcgggac gagtgcaaca tcgtcctgtg tgcgtctagg catagcggtg
     2821 gaactcgctc tttggttact ttcattttgg cgtcagccaa ggctttttca agtgaagaat
     2881 cctaacggat tcagcccgct gacgcttgct ccaatggcgg ccgcgtgtgc cagaccatgc
     2941 tggagcccgc tccggcgggg attaatgaca atgacttgtt ttgttttcat gcaacgaaca
     3001 agcccgctgt gctgtccacg acagcgattg tcacaagtca gacacaggcc gttcagccgt
     3061 gtgcacacgc atcggcatgg cgtacgacag aaaaaccaca ccgtgccatc tcctggcctg
     3121 acacatgcaa atcgctggcc agttttgcat cgacgtggcg cataaccatg gcgcacacgc
     3181 ccggcaacag tgcacgccag acatgccagt gtgcaaaaac gtcccagccc gagcaggccg
     3241 atagtaaaac cagcgaaaac gcttgacggc gtgtgccggc tcacgtaaaa ccttacttcg
     3301 ggttgcgagc ttgacctcgt tttgcagtac cggattgcct ccgcgatgct cgctaaaaca
     3361 caccacagtg aaagcttcgt agtatcgggg ttcccacccc tgttttttgt ttagggatgt
     3421 aacacatatg gcaactggta ccgtcaagtg gttcaatgac gctaaaggct tcggcttcat
     3481 caccccggac gatggtggcg aagacctgtt cgcgcacttc tccgcaatca atatgaacgg
     3541 cttcaaaacc ctgaaagaag gccaacgcgt gaccttcgaa gtgacccaag gtccgaaggg
     3601 taagcaagcc tccaacattc agtcccagta attgaactga gacggtttgc gtgagcacaa
     3661 cccgctaaag cgggtttttt catgcatatt tcgccagcgc agtggcagcc catgctgaat
     3721 aattcagggt ttctacctag tttcatacca gattcagggg aaattcgtca tcagtacaaa
     3781 tactgatctt gtcatcaagg gcttccgcgg gcatgaatat atgatataaa tgcccatgcg
     3841 ctttcggggg atggccccgt ttttttaagg aaacaagcaa gatggcaact ggtatcgtga
     3901 aatggtttaa cgacgcaaaa ggttttggct tcatcactcc ggatgatggt ggcgaagacc
     3961 tgtttgcgca cttctccgca atcaacatga atggcttcaa aaccctgaag gaaggcgagc
     4021 gcgtcagctt tgacgtcgcc caaggcccga agggcaagca agccgccaac atccagtcgg
     4081 ctagctaata gcccatggaa tgcagtgccc ggcgcgtttg gcgcgcaggg cacaggcaac
     4141 aaaaaacccg ccttccggcg ggttttttgt tgctggcgca gagattacat gcgctcgatc
     4201 atggcatcgc caaaggccga gcagctgact tcggtagcgc cgtccatcag acgggcaaag
     4261 tcatacgtca ccaccttgtc gccgatggct ttttccatcg agctgatgat caggtcggcg
     4321 gcttcggtcc agcccatgtg gcgcagcatc atttcggccg acaggatcag cgaacccggg
     4381 ttcaccttgt ccagaccagc gtacttcggc gcggtgccgt gggtggcttc aaacatcgcc
     4441 acgctgtcag acaggttcgc gcccggggcg atgccgatgc cgcccacttg cgcggccagc
     4501 gcgtcagaga tgtagtcgcc gttcaggttg caagtggcga tcacatcgta ttcggccgga
     4561 cgcagcagga tttgttgcag gaaggcgtca gcaatggcgt ctttgacgat gatgtccttg
     4621 ccggtcttcg ggttggtgaa cttgcaccac gggccgccat ccaccagctg ggcgccaaat
     4681 tcgttcttgg ccaggtcgta ggcccagtca cggaagccac cttcggtgaa cttcatgatg
     4741 ttgcccttgt gcacgatggt caccgacttg cgatcgttgt cgatggcgta ctggatggcc
     4801 ttgcgcacca ggcgctcggt gccttgcttg gacaccggct tgatgccgat gccggaggtt
     4861 tccgggaagc ggatcttctt cacgcccatt tcggtttgca ggaagttgat cagcttcttg
     4921 gcgccgtcgg tttcggcttg ccattcgatg ccggcgtaga tgtcttcggt gttttcacgg
     4981 aagatcacca tgtcggtcag gtccgggcgc ttcagcggcg agggcacgcc agtgaagtag
     5041 cgcaccgggc gcaggcagac gtacagatcc agctcctggc gcagggccac gttcagcgaa
     5101 cggatgccgc cgcccaccgg ggtggtcatc gggcccttga tcgacaccac gtagtctttc
     5161 agggcagcca tggtttcttc cggcagccac acatcgccgc cgtacacgcg ggtggatttt
     5221 tcaccggcgt agatttccat ccagtgaatt ttcttgctgt tgttgtaggc cttggacact
     5281 gcggcgtcca ccaccttgat catcaccggg gtgatgtcga tgccgatgcc gtcgccttcg
     5341 atgaacggga tgatcggatt gtcggggata gcctgacccg gaacgatttt ctggccgcct
     5401 tggggtacgg taatgtggga agtcgtcatg cctgctccat catggattgt agggtgggag
     5461 tgcgcgcgca tgttgcaaag cacccggacg ccggcttgtg gccgacagca gaatgacggc
     5521 tgcctgtggc ggggcagagg tcggacgggt tctcgacatg cgcatgaaca gatcgtgttg
     5581 gaatcaaatt cgggcgggga gagtgcgagc cggtcgcggc agagcgtgcg acggacaagt
     5641 tataggtatc gaaatttttc gcgtctcctc agcctgatat tatcgcgtaa aaccgttgcc
     5701 taagctagag gatgttcgat ttcgcgcatc catgcgctgt ttcgcatccg ccgcgccgcc
     5761 ttgttcatgc tgtcctgcaa catgaacaag gcatcaaatt gccagtattc accagaaagc
     5821 cccagatgtc ggaactgatc ctgttcaaca aaccttatgg cgtgatctgc cagttttccg
     5881 cgcaccccaa gcatcccagc ctggcggact atgtccgcat ccccgacgtt tatcccgccg
     5941 gccggctgga caccgacagc gaggggctgt tgctgctcac cgccgatggc gcgctgcaac
     6001 accagatcag ccatccggga tgcaaactgc caaaaaccta ctgggcccag gtagagggca
     6061 ttcccgacga cgacgcgctg gcccagctgc gcgccggggt ggatttgggc gactttgtca
     6121 cccagcccgc ccaggtgcgg cgcatcgacc cgcccagcct gtggccgcgc acgcccccca
     6181 tccgcgtgcg ccagagcgtg ccggacagct ggctggaaat catcatccac gaaggcaaaa
     6241 accgccaggt gcggcgcatg accgccaagg tgggccaccc cacgctgcgc ctggtgcgcg
     6301 ccgccatcgg cccctggtct ttgggcgact tgccgccggg cgagctgcgc cagctgaccg
     6361 tcagcgcgcc gcctcttgcc aatggcggca aaccagcgca ccatgaccgt gccatgtcgc
     6421 gagctgaccg cccggcccca ccccggcccg gcgtgcgcgc ccgtcatgcc ggtcgacgcc
     6481 gctgatccac gttgaccccg gcgcccgcat gggcgccgcg ccggcaagcc ctcacccgcg
     6541 cccttgcctt gtcacgccgg ccaactcgcc cgcgcatcag gcgtccgttc ggccggaact
     6601 ttgtccatcc tccgaccgcc gccgatgaac acaccgtccg aactcaacgc cgtagcctgg
     6661 gacattgccc agaccctgat caaaggcttt aacaagcact attttctgtt tcgcgaatgc
     6721 tcgcgcaacg ccaaggaata cttcgaccaa ggcgactggc acgccatgca gcgcgccatc
     6781 accgagcgca tccagtttta caccgaacgg gtgatggaaa ccgtcgaaca gctgcaatgc
     6841 cgcttccatg ccgacgcgct ggacgacagc acctggcagc aggtcaagct gtacttcatc
     6901 ggcatgctga tgaaccacaa acagccagag ctggccgaga cgtttttcaa ctcggtgttc
     6961 tgtcgtattc tggaccgcga ttatttcaac agtgatttca ttttcacccg cccggtgatt
     7021 tctacggaat atatcgagtc cgacccgccg atttaccgca gctattaccc cggccccaag
     7081 ggtctgtgcc agacgctgga acagattgtg gccgattttg gctggaaccg tccctttgcc
     7141 cacctcagac gcgacatcgt caaggtaatg cgcgccgcgc gcgagcatct gggcggccag
     7201 tgggccacac cggacgtcaa ttaccagatc caggtgctgt attctggttt ttaccgcaat
     7261 aaatccgcct acatcatggg caaggtgatc aacggcaatc aaagctaccc gtttgcgctg
     7321 tgcatcatgc acaacgacga cggcgagctg gaaatcgaca ccgtgctctt ggagccgtgg
     7381 cgcattggcg tgctgttctc gttcagccgc gcctacttcc tggtggacat ggaagtcccc
     7441 tcgggctatg tgcagttttt gcgcggcatg ctgccgtgga aaaccaaggc cgagatctac
     7501 accatgctgg gtctgcaaaa acagggcaag aacaccttct accgcgactt catgcaccac
     7561 ttgcagcact ccaacgatga tttcatcatc gcgccgggca ttcgcggcct ggtgatgctg
     7621 gtgttcaccc tgccgtctta ccccttcgtg ttcaagctga tcaaagatgt gtttggcagc
     7681 tcgaaagaag tggaccgccc caccgtcaag gccaaatacc tgctggtgaa gcgccacgac
     7741 cgggtgggcc gcatggccga cacgctggaa tactccaacg tcgccttccc gaaaaagcgc
     7801 tttaccgaag agctgctcac cgagctgcgc accctggcgc cgtcgctgct ggaagaaagc
     7861 gacgacaccc tggtgatcaa gcacttgtac atcgaacggc ggatgaagcc gctgaacatc
     7921 tacctggaca acgccacccc ggaagaaaaa gaacacgccg tgcgcgaata cggcgacgcc
     7981 atcaaagacc tggcccaggc caatatcttc tctggcgaca tgctgttcaa aaactttggc
     8041 gtgacgcgct atggccgggt gattttctac gactacgacg aaatcgagta catgaccgac
     8101 tgcaacttcc gggacatccc cgagccgccg tcgcccgagt acgaaatggc gtccgaaccg
     8161 tggtatccgg tggccaagaa cgatatcttc ccggaagaat tcgcctcttt cctgctgggc
     8221 gacccgctgg tgcgcaagtt cttcatcaag tatcaccgcg acctgctgtc gcctgctttc
     8281 tggcgcaccc agcagaccaa tatccgcgcc ggcaagatgg acaacttcta tccctatccg
     8341 caggcgttgc gcctgtgcca tcgctacccg gacaaagccg ccagcaagta agcgcggtgt
     8401 gttgatgtca gcctgaacgc gggcgggagg ccactcccgc ccgcgtttgc atctggcccc
     8461 c
//
LOCUS       DAOPYX010000120         8320 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_10241_length_8320_cov_13.709740, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000120 DAOPYX010000000
VERSION     DAOPYX010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 8320)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8320)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8320
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10241_length_8320_cov_13.709740"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            473..1111
                     /gene="hisG"
                     /locus_tag="PKN55_07510"
     CDS             473..1111
                     /gene="hisG"
                     /locus_tag="PKN55_07510"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003774004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HNX71127.1"
                     /translation="MLTIALSKGRIFDETLPLLAAANIIPAEDPESSRKLILATNHPD
                     VRLVIVRATDVPTYVQHGAADLGIAGRDVLIEHGGAGLYQPLDLNIACCKMMVAAPNG
                     FDYAAAVQHGARLSVATKYPNIAREHFAAKGVHVDVIKLYGSMELAPLVGLADAIVDL
                     VSTGGTLKANNLTPVEHILDISSQLIVNQAALKLKHAAIRPVIDAFAATVKR"
     gene            1139..1753
                     /locus_tag="PKN55_07515"
     CDS             1139..1753
                     /locus_tag="PKN55_07515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012695674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HNX71128.1"
                     /translation="MWETVLTITVIATLAMISPGPDFVLVVKNAARYSRRAALATTVG
                     INLGIAVHMSYCILGLALIIAQTPWLFGLLKYAGAGYLIWIGVQALFSRGGLAAATAP
                     GGPERASVRRAFLEGLLCNLLNPKATLFFFSVFTQLLRPDSTLTEKALIGLVIFLLGV
                     IYWPCVALAVQHPRVMALLHRAQRRIDRLLGGVLVALGVKVALS"
     gene            1794..3086
                     /gene="hisD"
                     /locus_tag="PKN55_07520"
     CDS             1794..3086
                     /gene="hisD"
                     /locus_tag="PKN55_07520"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HNX71129.1"
                     /translation="MHMLDSRQPGFDAALRQLLAFETAQDPAVDAAVADIIASVQSQG
                     DAALIDYTARFDRLSVTDASQLELSQAELDAALHRLPAATRAALEAAAERVRRYHERQ
                     LMQSWSYVEEDGTRLGQQVTALDRVGIYVPGGKAAYPSSVLMNAIPAKVAGVGEIIMV
                     VPTPGGERNDIVLAAARIAGVDRVFTVGGAQAVAALAYGTQTIPAVDKITGPGNAYVA
                     AAKRRVFGVVGIDMVAGPSEILVICDGQTDPDWIAMDLFSQAEHDEIAQAILLCPDAD
                     FITRVRASIEHLLPEMPRQDIIRASLSGRGALIQVADLDEACQIANYIAPEHLELSVA
                     EPAAWLPKLKHAGAIFMGRFASESLGDYCAGPNHVLPTSRTARFASPLGVYDFQKRTS
                     LIEVSAVGAQTLGRIASTLAHGEGLTAHARSAEFRLQD"
     gene            3317..4408
                     /locus_tag="PKN55_07525"
     CDS             3317..4408
                     /locus_tag="PKN55_07525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011133593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNX71130.1"
                     /translation="MPRYARHLAEHTIVNPFPGIVKLERRLGHAVPVRIGSNEGLAEP
                     RSPLEAAFGPALAELARLYPDPYALTLRERLAALNHCQPEEIIVDAGADSLILLALRL
                     CVNPGDTVVCTAGSYPTFRYFAEGVGAQLREIPYQAHAGGLRPDWQALVEAANAEQAA
                     LLYLANPDNPTGHLWSGEVVAQLRAALNPDTLLLLDEAYLDFAEPTERPADGPLPGVL
                     RLRTLSKAYALAGARVGYALADAALIDKADQIRPQFAVSSWAQAAAHTVLADPDYAPA
                     LRAHTLALREALAEALRARGRQPFASATNFVCVACADRAEAEGIQARLLAAGVALHRP
                     PHPAVGHLLRITAHPAALSEDVLAVLQKP"
     gene            complement(4560..5645)
                     /locus_tag="PKN55_07530"
     CDS             complement(4560..5645)
                     /locus_tag="PKN55_07530"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011134564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_function: GO:0050568 - protein-glutamine glutaminase
                     activity [Evidence IEA];
                     GO_process: GO:0006482 - protein demethylation [Evidence
                     IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0018277 - protein deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator protein-glutamate
                     methylesterase"
                     /protein_id="HNX71131.1"
                     /translation="MSRKIRVLVVDDSAVVRQVVTQTLSSDPGIEVIGAALDPVFALE
                     KMKTNWPDVIVLDLEMPRMDGLTFLRRIMAERPTPVVICSSLAEKGAQVALDALAAGA
                     VSIITKPKVGLKSFLDGAANDIIQAVKAAAHSRPGRRASAAVASLSPSPSLSADVILP
                     AAHNASAPLGRTTDRLVAIGTSTGGTQALEAVLTALPVDTPGVVVVQHMPEKFTAMFA
                     QRLNSLCKIEVREARSGDRVLPGLALIAPGGRHMMVKRNGAQYVVDVVDGPLVNRHKP
                     SVDVLFRSVAKCAGHNALGIIMTGMGDDGARGLKEMRDAGAHTLAEDESTCVVFGMPK
                     EAIRLGGAAEVLPLTRMAEGIGRFTRS"
     gene            complement(5642..6157)
                     /locus_tag="PKN55_07535"
     CDS             complement(5642..6157)
                     /locus_tag="PKN55_07535"
                     /inference="COORDINATES: protein motif:HMM:NF015908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheD"
                     /protein_id="HNX71132.1"
                     /translation="MPDLFASTDPHTVFLHPGEFYFGGGPTRIATLLGSCVSITVWHP
                     RLLVGGMCHYMLPSRQRPPQAALCGRYGDEAMEWLLARIRETGAPAAQFQAKLFGGGN
                     MFETAIGGVMDIGRRNIDMGRQLLTQAGISLIAEHVGGNAHRKLVFELWSGDVWLARP
                     DRYASLGDALS"
     gene            complement(6171..6986)
                     /locus_tag="PKN55_07540"
     CDS             complement(6171..6986)
                     /locus_tag="PKN55_07540"
                     /inference="COORDINATES: protein motif:HMM:NF013867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HNX71133.1"
                     /translation="MALETISSADFDRFRRLLYEHSGIHLADNKQPLLIGRLGRRLRE
                     LGLDSYADYYQRVTQPGCEVERQRMIDLLTTNETHFFREPQHFVYLREHILPRHPAGT
                     PFRAWSAASSTGEEAYTLAIELAEAFGEAPWEIIGTDISHRVVEQARLGIYPIERAKE
                     IPIPLLKKYCLRGVRSQDGAMRVMSSLTRRAHFLPANLLAPLPDIGRFDVIFLRNVMI
                     YFDMETKRRVVDNVLTRLKPGGTLFIGHAETLQNVSDRVQLVKPTIYRLLAHT"
     gene            complement(7131..7670)
                     /locus_tag="PKN55_07545"
     CDS             complement(7131..7670)
                     /locus_tag="PKN55_07545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014086712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HNX71134.1"
                     /translation="MGQTHQDAAANLSATQQFLTFSLSGEMFGVGILTVKEIIEYGEV
                     TEIPMVPAFICGVINLRGAVVPVIDLAARFGNTPTIPSRRTCIIILEIRHEDSTQTLG
                     VLVDSVSAVLDIPTSEIEPPPAFGARIRADFIVGMGKVDGHFVILLDIERVLSVDEIA
                     TLASLGESDTSEAVSASLN"
     gene            complement(7695..>8320)
                     /locus_tag="PKN55_07550"
     CDS             complement(7695..>8320)
                     /locus_tag="PKN55_07550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018151815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNX71135.1"
                     /translation="AMQEIASRIGIIDDIAYQTNLLALNAAIEAARAGEHGKGFAVVA
                     AEVRKLAERSQVAAQEISQLAGSSVKMAGRAGELLTEMVPTIRKTSDLVQEIAAASSE
                     QSSGVSQINGAMGQLNQATQQNASASEQLAATAEELGSQAEQLQELMTFFHLGQDDGN
                     RRSSSKITVKPASRSSHSHPSAVRAPAAAASLPPAGAAVNEQDFERF"
BASE COUNT         1399 a         2720 c         2666 g         1535 t
ORIGIN      
        1 agtcgcttgg gcagatgggc caattggcag ctccttgcag tgagtgcaac gtatgattga
       61 aagccggatt ataccgggct aaatataaaa cggggctgtt ctgaatcagc tttgcgtctg
      121 gcaaattaaa attctgatag cattttaaat gggtcgcggg cctgttgcac cctgctaaaa
      181 ttacatatat attgaaaatc attgctttat cttcatgtag caagcgctga cttgcgcatc
      241 gcccacgcag atggtgagat gatttgtgtc tgccagcaaa aacaatcatg acgtataaaa
      301 atgccattgc acttgccgct caaggaaata taaattttta tatagaatat atttgtctga
      361 tgtgtttttt gtcggggcgt ggctgtcgtg cttcttgctc tgccgcaccg gcctgtccat
      421 ttgcgcctga tcccgttaaa atcagtcttt tgcttcagac ggccttgccc gcatgctgac
      481 cattgccctt tccaaggggc ggattttcga tgaaaccctg ccgttgctgg cagcggcgaa
      541 catcatcccc gccgaagacc cggaatcctc ccgcaagctg attctggcca ccaaccaccc
      601 ggatgtgcgc ctggtgatcg tgcgcgccac cgacgtgccc acctatgtgc agcatggcgc
      661 cgccgacctg ggcattgccg ggcgcgacgt gctgatcgag cacggcggcg ccggcctgta
      721 tcagccgctg gatctcaaca tcgcctgctg caagatgatg gtggccgcgc ccaatggctt
      781 tgactacgcc gccgccgtgc agcatggcgc gcgcctgtcg gtggccacca aataccccaa
      841 catcgcccgt gaacactttg ccgccaaggg tgtccatgtc gatgtgatca agttgtacgg
      901 ctcgatggag ctggccccgc tggtcggcct ggccgacgcc attgtcgacc tggtgtccac
      961 cggcggcacg ctgaaagcca acaacctgac cccggtggag catattctgg acatcagctc
     1021 gcagctgatc gtcaaccagg cggcgctcaa gctcaagcac gccgccatcc gcccggtgat
     1081 cgacgccttc gccgccacgg tcaagcgctg agtcacccgg ccagccagga gcgcgcgcat
     1141 gtgggaaacc gtcctcacca tcacggtgat cgccaccctg gcgatgatca gccccggccc
     1201 ggattttgtg ctggtggtca aaaacgccgc gcgctactcg cgccgcgccg cgctggccac
     1261 cacggtgggc atcaatctgg gcattgccgt gcacatgagc tattgcatcc tgggcctggc
     1321 gctgatcatt gcgcagacgc cctggctgtt tggcctgctc aagtatgccg gcgccgggta
     1381 tctgatctgg attggcgtgc aggcgctgtt cagccgtggc ggcctggccg ccgccaccgc
     1441 gcccggcggg cctgagcgcg ccagcgtgcg ccgcgccttt ctggaagggc tgctgtgcaa
     1501 tctgctcaac cccaaggcca cgctgttttt ctttagtgtg ttcacccaat tgctgcgccc
     1561 ggactccacc ctcaccgaaa aagcgctgat tggcctggtg attttcctgc ttggcgtcat
     1621 ctactggcca tgtgtggcgc tggcggtgca acatccgcgc gtgatggcgc tgctgcaccg
     1681 tgcgcaacgc cgcatcgacc gcctgctggg cggcgtgctg gtggcgctgg gcgtcaaggt
     1741 ggcgctgtcc tgaagcgccc ggatttcatt ttttgcagga tgcctgactc atgatgcaca
     1801 tgcttgattc ccgtcagccg ggctttgacg ccgcgctgcg ccagctgctg gcctttgaaa
     1861 ccgcccaaga cccggcggtg gacgccgccg tcgccgacat catcgccagc gtgcaaagcc
     1921 agggcgacgc cgcgctgatc gactacaccg cccgctttga ccgcctgagc gtcaccgacg
     1981 ccagccagct ggagctcagc caggctgaac tcgacgccgc gctgcaccgc ctgcccgccg
     2041 ccacccgcgc cgcgctggaa gccgccgccg agcgcgtgcg ccgctaccac gagcgccagc
     2101 tgatgcaatc gtggagctat gtcgaagaag acggcacccg gctgggccag caagtcaccg
     2161 cgctggaccg cgtgggcatt tatgtgcccg gcggcaaggc ggcttacccc tcgtcggtgc
     2221 tgatgaacgc catcccggcc aaggtggccg gcgtgggcga aatcatcatg gtcgtcccga
     2281 cccctggcgg cgagcgcaac gacatcgtgc tggccgccgc gcgcattgcc ggcgtggacc
     2341 gcgtgttcac cgtcggcggc gcccaggccg tggcggcgct ggcctacggc acgcaaacca
     2401 tcccggcggt ggacaaaatc accggccccg gcaacgccta cgtggctgcc gccaagcgcc
     2461 gggtgtttgg cgtggtcggc attgatatgg tggccggccc gtcggaaatc ctggtgatct
     2521 gcgacggcca gaccgacccg gactggattg ccatggactt gttcagccag gccgagcacg
     2581 acgaaatcgc ccaggccatc ctgctgtgcc cggacgccga tttcatcacc cgtgtgcgcg
     2641 ccagcatcga gcacctgctg cccgagatgc cgcgccagga catcatccgc gcctcgctgt
     2701 ccggccgtgg cgcgctgatt caggtggccg acctggatga agcctgccag atcgccaatt
     2761 acatcgcccc ggaacacctg gaattgtccg tcgccgagcc ggccgcctgg ctgcccaagc
     2821 tcaagcatgc tggcgccatc ttcatgggcc gcttcgcctc ggaaagcctg ggcgactact
     2881 gcgccggccc caaccacgtg ctgcccacca gccgcaccgc ccgctttgcc agcccgctgg
     2941 gcgtgtacga cttccagaag cgcaccagcc tgattgaagt ctctgcggtg ggcgcgcaaa
     3001 cgctgggccg catcgccagc accctggccc acggcgaagg cctgaccgcg cacgcgcgca
     3061 gcgccgaatt ccgcctgcaa gactgagcgc gccccacggg cttgttctct cacgccgccg
     3121 cgctttccgg tctggaaggc gcggcggcgt tgttttgtgc gtggtgggct gaacgatttt
     3181 tgcctgccct tgtggcggca agcgtgacgc tgacagcaag cgccctcgcc cggcataagc
     3241 tcagcacatt ccggacatgc gcgccgtccc cggctgggcg atactggcgg ctccgccgtt
     3301 tttcaggagc cgcctcatgc cccgttacgc ccgccatctg gccgagcaca ccatcgtcaa
     3361 cccgtttccc ggcatcgtga agctggagcg ccggctgggc catgccgtgc cggtgcgcat
     3421 tggctccaac gaaggcctgg ccgagccgcg ttcgccgctg gaggccgctt ttggcccggc
     3481 gctggccgag ctggcgcggc tctaccccga cccctacgcg ctgactttgc gcgagcgcct
     3541 ggcggcgctg aaccactgcc agccggaaga aatcatcgtc gatgccggcg ccgacagcct
     3601 gatcctgctg gcgctgcgcc tgtgcgtgaa ccccggcgac accgtggtgt gcaccgccgg
     3661 cagctacccg acctttcgct attttgccga gggcgtcggc gcgcagctgc gggaaatccc
     3721 ctaccaggcg catgccggcg gcctgcgccc ggactggcaa gcgctggtgg aggcggccaa
     3781 tgccgaacag gcggcgctgc tctacctggc caacccggac aaccccaccg gccatctgtg
     3841 gtcgggcgag gtggtggccc agttgcgcgc cgcgttgaac ccggacacct tgctgttgct
     3901 ggacgaagcc tatctggact ttgccgaacc caccgagcgc ccggccgatg gcccgctgcc
     3961 cggcgtgctg cgcctgcgca ccttgtccaa ggcctatgcg ctggccggcg cgcgggtggg
     4021 ctacgccttg gctgacgcgg cgctgatcga caaggccgac cagattcgcc cgcaatttgc
     4081 cgtgtccagc tgggcgcagg cggcggcgca caccgtgctg gccgacccgg actacgcccc
     4141 ggccctgcgc gcccacaccc tggcgctgcg cgaggcgctg gccgaggcct tgcgcgcgcg
     4201 cggccgccag ccgtttgcct cggcgaccaa ttttgtgtgt gtggcctgcg ccgaccgcgc
     4261 cgaagccgag ggcattcagg cgcgtctgct ggcggcgggc gtggcgctgc accggccgcc
     4321 gcacccggcg gtggggcatt tgctgcgcat caccgcccac ccggcggcct tgtcggaaga
     4381 cgtgctggcg gtgttgcaaa aaccatgagc ctgacgccgt cgccttgccg gtgcgctctg
     4441 ctcaggccaa gtggtttgag ggcggccgcg gtgtgattgt gctgtgccgg gcataaaaaa
     4501 agcgctggcc ccaacacggg aagccagcgc aagcacggta gagtgaagca aaacggtcgt
     4561 caactgcggg taaagcggcc gatcccttcg gccatgcgcg tcagcggcag cacttccgcc
     4621 gcgccgccca ggcggatggc ctctttgggc atgccaaaca ccacacaggt ggattcgtct
     4681 tcggccaggg tgtgcgcgcc agcgtcgcgc atttctttca ggccgcgcgc gccatcgtcg
     4741 cccatgccgg tcatgatgat gcccagcgca ttgtggccgg cgcatttggc caccgagcgg
     4801 aacagcacat ccaccgaggg cttgtggcgg ttgaccagcg ggccatcgac cacgtccacc
     4861 acgtattgcg cgccattgcg cttgaccatc atgtgccggc cgccaggggc aatcagcgcc
     4921 aggccgggca gcacgcggtc gccgctgcgc gcctcgcgca cttctatctt gcacaggctg
     4981 ttcagccgct gggcgaacat ggcggtgaat ttttctggca tgtgctgcac caccaccacg
     5041 ccgggggtgt ccaccggcag cgcggtgagc accgcttcca gcgcctgggt gccgccggtg
     5101 gaggtgccaa tcgccaccag ccggtcggtg gtgcggccca gcggggccga ggcgttatgg
     5161 gccgcgggca ggatcacgtc agccgacaga ctgggcgagg gcgacaggct ggccaccgcc
     5221 gcgctggcgc ggcggccagg gcgcgagtgg gcggcggctt tcaccgcctg gatgatgtcg
     5281 ttggcggcgc catccagaaa ggacttcagc ccgaccttgg gcttggtgat gatgctcacc
     5341 gcgccggccg ccagcgcatc cagcgccact tgcgcgcctt tttccgccag ggaggagcag
     5401 atcaccaccg gcgtggggcg ttcggccatg atgcggcgca aaaaggtcag cccgtccatg
     5461 cgcggcattt ccaggtcgag cacaatgacg tccggccagt tggttttcat tttttccagc
     5521 gcgaatactg ggtccagcgc ggcgccgatc acttcgatgc cagggtcgct ggacaaggtt
     5581 tgggtgacaa cctgacgcac cacggcggaa tcatccacca ccagcacacg gattttgcgg
     5641 ctcatgacaa tgcgtctccc aaagaggcgt agcggtcggg gcgcgccaac cagacgtcgc
     5701 cgctccagag ttcaaacacc agttttcggt gggcgttgcc gcccacatgc tcggcgatca
     5761 ggctgatgcc agcctgggtc agcagctgcc ggcccatgtc gatattgcgc cggccaatgt
     5821 ccatcacccc gccgatggcg gtttcgaaca tattgccgcc gccaaacagc ttggcctgaa
     5881 actgcgcggc cggcgcgccg gtttcgcgga tgcgcgccag cagccattcc atggcttcat
     5941 cgccataacg gccgcacagc gccgcctgtg gcgggcgctg gcggctgggc agcatgtagt
     6001 gacacatccc acccaccagc aagcgcggat gccagaccgt aatcgacacg cacgagccca
     6061 gcagggtggc gatgcgggtg gggccgccgc caaaataaaa ctcaccgggg tgcaagaaca
     6121 cggtgtgggg gtcggtgctg gcaaaaaggt caggcatagc gcatccggcg tcaggtgtgc
     6181 gccagcaggc ggtagatggt aggtttgacc aactgcaccc ggtcgctgac gttttgcagg
     6241 gtttcggcat ggccgatgaa caaggtgccg ccgggcttga gccgggtcag cacgttgtcc
     6301 accacccgcc gtttggtttc catatcgaag taaatcatca cattgcgcag gaagatcacg
     6361 tcaaaacggc caatatcggg caatggcgcc agcagattgg ccggcaggaa gtgcgcccgg
     6421 cgggtgagcg aactcattac ccgcatggcg ccatcctggc tgcgcacacc gcgcagacaa
     6481 tatttcttca gcaatggtat tggaatttcc ttggcgcgtt caatcgggta aataccgagt
     6541 cgggcttgct caaccacacg atggctgatg tcggtgccga tgatttccca cggtgcttcg
     6601 ccaaatgcct cggccagctc aatcgccagg gtataggctt cttcgccggt ggagctggcc
     6661 gcgctccagg cgcgaaatgg cgtgccggcc gggtgtctgg gcaggatgtg ctcgcgcaaa
     6721 tagacaaaat gctgcggttc gcggaaaaaa tgggtttcgt tggtggtcag caggtcgatc
     6781 atgcgctgac gctcgacttc gcagccgggc tgggtgacac gctgataata gtcggcgtag
     6841 gaatccagcc ccagctcacg cagccggcgc cccagccggc cgatcagcaa gggttgcttg
     6901 ttatcagcca gatgaatgcc gctgtgttcg tacagcaggc ggcgaaagcg gtcgaagtcg
     6961 gcagaggaaa tggtttccag cgccatggcg tgtccttccc ggcagaatca tcgcgtcccc
     7021 gtccgaagaa cgggggaaaa cgactagtgt gcctgagatt aggacaaatg ggaaatttcg
     7081 tcggcggctg agggcggcgg aacgggcgcc ggctggcgcc cgttcgtgtg tcagttgagg
     7141 ctggcgctga cggcttcgct ggtgtcggat tcgcccagcg aggccagggt ggcgatttcg
     7201 tccaccgaca gcacccgctc gatgtcgagc agaatgacaa aatggccatc caccttgccc
     7261 atgccgacga tgaaatccgc gcggatgcgc gcgccaaagg ccggcggcgg ttcgatctcg
     7321 ctggtgggaa tgtccagcac cgccgacacg ctgtccacca gcacgcccag ggtttgcgtg
     7381 ctgtcttcgt ggcggatttc cagaatgatg atgcaggtgc gccgtgacgg aatggtgggc
     7441 gtattgccaa agcgggcggc cagatcaatc accggcacca cggcgccgcg caggttgatc
     7501 accccacaaa taaaggccgg caccatggga atctcggtga cctcgccgta ttcaatgatt
     7561 tccttcaccg tcaggatgcc gacgccaaac atctcgccag acaggctgaa ggttaaaaac
     7621 tgctgagtgg ccgacaggtt ggcggccgcg tcttggtgtg tttgtcccat ggtggataat
     7681 ccttcccgct gcgctcagaa ccgctcgaaa tcctgctcat tcactgctgc accagccggc
     7741 ggcaagcttg ctgccgctgc gggtgcgcgc acggccgagg ggtggctgtg gctgctgcgg
     7801 ctggccggct tgacggtgat tttgctgctg ctgcgacggt tgccgtcatc ttgacccaaa
     7861 tggaagaagg tcatcaactc ttgcaactgt tcggcctggc tgcccagctc ttcggcggtg
     7921 gcggccagtt gctccgaggc cgaggcgttt tgctgggtgg cctgattgag ctggcccatg
     7981 gcgccattga tctgcgatac gcccgacgat tgctcgctgg aggcggcggc gatttcctgc
     8041 accagatcag atgtcttgcg aatggtcggc accatttcgg tcagcagctc accggcacgg
     8101 ccggccatct tcacgctgga gcctgccagt tggctgattt cctgggcggc tacctggctg
     8161 cgttcggcca gtttgcgcac ctcggcggcc accacggcaa agcccttgcc gtgctcgccg
     8221 gcgcgggcgg cttcgatggc ggcgttcaag gccagcagat tggtttggta ggcgatgtcg
     8281 tcgatgatgc cgatgcgcga ggcgatttcc tgcatggcgt
//
LOCUS       DAOPYX010000121         8252 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_10358_length_8252_cov_10.722093, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000121 DAOPYX010000000
VERSION     DAOPYX010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 8252)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8252)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8252
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10358_length_8252_cov_10.722093"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            279..2087
                     /locus_tag="PKN55_07555"
     CDS             279..2087
                     /locus_tag="PKN55_07555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019924797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I secretion system permease/ATPase"
                     /protein_id="HNX71136.1"
                     /translation="MKKHLTPRTELAETLYGFRKTLYQLGAFSFVINLLGLMPSIYML
                     QVYDRVLASSNETTLLMLTLMTLGLYALMELLELARSSVLIRVGNRLDMQLNQRVFTA
                     SFERNLKRAGGNPSQAIHDLTSVRQFLTGNGLFAFFDAPWMPIYLLVCFMFHPLLGLV
                     VLIGALLLFGLAYLTEVSTRKPLADANQAAMASSAFANNNLRNAEVIEAMGMLPAIRE
                     RWFGYHRRILDNQTLASDRNARINVMTKFIRISLQSLILGLGALLVIEGKLTPGMMIA
                     CSILMGKALQPVELAIGTWKQLLSARTSYARLDDLLKEAPPRGAGMPLPPPAGNMTVE
                     GVIAVPPGGQHAVVRGLTFAMSAGDVVGVIGPSASGKSTLARLLVGVWPTQAGKVRLD
                     GADIFQWNKDQLGPWIGYLPQDIELFEGTIAENIARFGKVDSDQVISAAQRAGVHDMI
                     LRFPQGYDTRLGLDGGMLSGGQKQRIGLARALYGDPSLIVLDEPNSNLDDVGENALVR
                     AVMDLKARGKTVVLITHRTSIINAVDKLLVMREGEMVVYGPRDQVLAHLMQQQQQAQQ
                     AAQQAAQARQAPPAPVQPAQPQVAQNPAAPAEDAAE"
     gene            2113..3477
                     /locus_tag="PKN55_07560"
     CDS             2113..3477
                     /locus_tag="PKN55_07560"
                     /inference="COORDINATES: protein motif:HMM:TIGR01843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030253 - protein secretion by the type I
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family type I secretion periplasmic adaptor
                     subunit"
                     /protein_id="HNX71137.1"
                     /translation="MNWLKRLKGPTEAQTDSTAPGAPAVVDVDAHAPARWGMLVLGVG
                     FGGFLLWAAFAPLDAGVSSGGTVKVASSRKTIQHLTGGIVDTIDVKEGQVVKKDQVLV
                     KLNPTNANAQLGIVQAQFLAAQAIADRLSAEREGKTTVTFSPVLTEKFGNDSRAKDAM
                     ALQAQLFASRRAALQAELGILNENSQGMQEQLKGLQSLKDSREQQLKFIQEELKGVRD
                     MAAQGYVPRNRMLQLERNVAEISGQLASNIGDIGRSKNALSEIKLRMLQRQKEYQKEV
                     ETQLSDAQKEARAQGDRLAALEYELSNTQIKSPIDGTVVGVNVHTVGGVIQPGFHIMD
                     VVPLNEPMIVEARIQTNLIDKVHPGLPVDLTFPAFSHAQTPNIPGVVDTVGADALTDE
                     VTRMPYYPMQVKVTPEGMKILGKLQIRPGMPATVVVKTGERTMLNYLLKPLIDRVSGA
                     FKEE"
     gene            3480..4883
                     /locus_tag="PKN55_07565"
     CDS             3480..4883
                     /locus_tag="PKN55_07565"
                     /inference="COORDINATES: protein motif:HMM:TIGR01844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family outer membrane protein"
                     /protein_id="HNX71138.1"
                     /translation="MRLRKLTLLTALALSLNPLLPASALGFMEAYRAALQNDPTYQAA
                     IAENEAGQQNRAMGRAGLLPQISANASRARVRGEREYNTQTLSGVRNVIEPLSYFSTS
                     RTVQVRQSVLNMAAIANFRQGRARAEYSTEVFRGKQNDLALRLSSAYFQALLSQHAID
                     LAQAKVQSIEQQLKTAEVQFKAGDGTVTDVDEARARRDLAQAQLIEARNQLTVAMRSL
                     QELVGDKPVSLAQLKADFAKQLKPELETLDTWLDRALETSADIAAQRRALEVAEREVD
                     KNRAGHLPTVDVYASSGRSQSDSFSSINQEYRTRQLGVQVNIPIFSGGYTNAATTQAI
                     ANRERARNELEATVNKTHIEVTKQYSGTVSGLAKVQALELAVKSSEQALESTKMGFKA
                     GMRSNVDILNAEEQLYTSRRDLAEAKYVYLLSRLRLKAAAGQLSEADMGEVDSYFGAE
                     KPVHEKLVQARRSANGG"
     gene            4957..5508
                     /gene="rfbC"
                     /locus_tag="PKN55_07570"
     CDS             4957..5508
                     /gene="rfbC"
                     /locus_tag="PKN55_07570"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008952446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HNX71139.1"
                     /translation="MQIVEHALNGVLLIEPRVFGDERGFFFESYNQQAFAAACGQTVP
                     FVQDNHSRSSANVLRGLHYQIEHAQGKLVRVIHGEVFDVAVDLRRSSPSFGQWTGHYL
                     SAANKRQLWVPAGFAHGFLVVSETAELLYKTTDFYRPEHERCIAWNDPQLNIRWPLPE
                     GIAPIVSAKDRVGLAFADAPSYP"
     gene            5505..6386
                     /gene="rfbD"
                     /locus_tag="PKN55_07575"
     CDS             5505..6386
                     /gene="rfbD"
                     /locus_tag="PKN55_07575"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008952448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HNX71140.1"
                     /translation="MSARPILLIGAHGQLGRELARSLAVLGPLHALGRAELDLTDADA
                     IARVVDDVRPGLIVNAAAYTAVDRAESEPELAFAINARAPGLLAEAAARLDGGLVHYS
                     TDYVFSGEGATPWRESDATAPVNVYGASKLAGEQAVLASGADALILRTSWVYGGPGQN
                     FVNTMLRLAGERERLTVVDDQIGAPTASRFLADATLAALAQDWRARAGLYHLSASGDT
                     SWCGFARAIVEQALAHGQALKLSAADIAPIPSSSYPTPARRPANSRLDCSAFEQAFAV
                     HRPHWRAQLAAHFASRG"
     gene            6435..7211
                     /gene="lgt"
                     /locus_tag="PKN55_07580"
     CDS             6435..7211
                     /gene="lgt"
                     /locus_tag="PKN55_07580"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HNX71141.1"
                     /translation="MIWHWQFDPIALHVGPLQLHWYGVLFALAFVLGQQLLAWCYRRE
                     GYDSQALDSLMPWALIGTVLGARLAHCLIYDPAYYLSQPLAILRVWEGGLASHGGALG
                     LMLALWLYSRRHPQPGFAWLLDRIALPAALGGALVRCANFLNSEILGRPTGGDWGVVF
                     AAIDPLPRHPVQLYEAGAYLLIAALLWTVYRRHGRATPSGLLAGLFLLTVFAARALLE
                     TLKTPQAAYETGFAVSVGQWLSLPLMALGLWLVWRAKRRA"
     gene            complement(7226..8158)
                     /locus_tag="PKN55_07585"
     CDS             complement(7226..8158)
                     /locus_tag="PKN55_07585"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HNX71142.1"
                     /translation="MSAWLDSWDSFIKVVESGSMAGAARRLGCSRAQVSRQIGQLEAA
                     LGARLFERSTRRLRPTPAGEAFYPHALAAVAAVDCAQRALSHLGSAPRGLLRISATMT
                     FGREYIAPLLPALTERHPELECELILTDQMVDLVDDRIDLALRQTQTPPADAVAKPLL
                     QMERVICAAPDYLARHGTPEHPLQLAQHQCFSYLLTDDGHWRLRDARGEEWSAPVRGR
                     VQYNNIDCLYQATLAGQGLSILPVYLCAPALADGRLRQVLPDCQPQVRFGQHLYACYT
                     PSRVQLPKVQVFLAALEDLLQPEPPWRQIWPGPG"
BASE COUNT         1494 a         2698 c         2649 g         1411 t
ORIGIN      
        1 cgcgcgtggt gggcgacaac cgcaaattgt gcgccgacac ggggtgggcg ccgcgggtga
       61 gcatggccga cagcctgcgt gaaatcctcg acgactggcg cgcgcgttag cctctaagcc
      121 ggggcggcgg ccccctacaa accgttacaa accgtcgcaa tcctcgcatg gtaaactgcg
      181 cgccgttgtg gtgagttccc ttgggcgtgc gcctttctca ctgcgcaacc gtccggcgcg
      241 ggcgtgccgc cctggttcat tcctctcttg acacactcat gaaaaaacat ctgacacccc
      301 gcacggagct ggccgaaacg ctgtacggct tccgtaaaac cctttaccag ttgggcgcat
      361 tcagctttgt catcaacctg ctggggctga tgccgtccat ttacatgttg caggtctatg
      421 accgcgtgct ggccagcagc aacgaaacca cgttgctgat gctcacgctg atgacgctgg
      481 gcctgtacgc gctgatggag ttgctggagc tggcgcgctc gtcggtgctg atccgcgtgg
      541 gcaatcgcct ggacatgcag cttaaccagc gcgtgttcac cgcttcgttt gaacgcaacc
      601 tcaagcgcgc tggcggcaac ccttcgcaag ccattcacga tctgaccagt gtgcgtcagt
      661 ttctgaccgg caatggcctg ttcgccttct ttgatgcgcc gtggatgccg atttatctgc
      721 tggtgtgctt catgttccac ccgctgttgg ggttggtggt gctgatcggc gcgctgttgc
      781 tgtttggcct ggcttacctg accgaagtgt ccacccgcaa gccgttggcc gacgccaacc
      841 aggcggccat ggcctcgtcg gctttcgcca acaacaatct gcgcaatgcc gaagtgatcg
      901 aagccatggg catgctgccg gccatccgcg agcgctggtt tggctatcac cgccgcattc
      961 tggacaacca gaccctggcc tctgaccgca acgcccgcat caatgtgatg accaagttca
     1021 tccgcatcag cctgcagtcg ctgattctgg gcctgggcgc cttgctggtg atcgaaggca
     1081 agctgacccc cggcatgatg atcgcctgct cgatcttgat gggtaaggcg ctgcagccgg
     1141 tggagctggc gattggcacc tggaagcaac tgctgagcgc gcgcacctcg tacgcccgtc
     1201 tggacgatct gctcaaagaa gccccgccgc gcggcgccgg catgccgctg ccgcccccgg
     1261 ccggcaatat gacggtggaa ggcgtgatcg ccgtgccgcc gggcggccag cacgcagtgg
     1321 tgcgcggcct gacctttgcc atgagcgcag gcgatgtggt gggcgtgatt ggcccgtccg
     1381 cttcgggcaa atccaccctg gcgcgtttgc tggtgggcgt gtggccgacc caggcgggca
     1441 aggtgcgcct ggacggcgcc gacatcttcc agtggaacaa agaccaactg ggcccgtgga
     1501 ttggctacct gccgcaggat atcgagctgt ttgaaggcac cattgccgaa aacatcgccc
     1561 gctttggcaa ggtggactct gaccaagtca tcagcgccgc gcagcgcgcc ggcgtgcacg
     1621 acatgatcct gcgcttcccg cagggctacg acacccgtct gggcctggat ggcggcatgc
     1681 tgtccggcgg ccagaagcag cgcattggcc tggcgcgcgc gctgtatggc gacccgtcgc
     1741 tgatcgtgct ggacgaaccg aactccaacc tggatgacgt gggtgaaaac gccctggtgc
     1801 gcgccgtgat ggacctgaaa gcgcgcggca agaccgtggt gctgatcacc caccgcacca
     1861 gcatcatcaa cgcggtcgac aaattgctgg tgatgcgcga aggcgagatg gtggtgtatg
     1921 gcccgcgcga ccaagtgctg gcgcacctga tgcagcagca acagcaagcg cagcaggcgg
     1981 cgcaacaagc cgcccaagcg cgccaggcgc cgcctgcgcc tgtgcagcct gcccagccgc
     2041 aagtggcaca aaacccggcc gccccggcag aagacgccgc cgagtaaccg cacgctttga
     2101 agaagaattg aaatgaactg gcttaaacgc ttgaaagggc cgactgaggc tcagaccgat
     2161 tcgaccgcgc ccggcgcgcc tgctgtggtg gatgtggatg cgcacgcgcc tgcgcgctgg
     2221 ggcatgctgg tgctgggcgt cggctttggc ggcttcctgc tgtgggcggc ttttgcgccg
     2281 ctggacgcag gggtgtcctc ggggggcacg gtcaaagtgg cctccagccg caagaccatt
     2341 cagcatctga ccggtggcat tgtggacacc atcgacgtca aagaaggcca ggtggtcaaa
     2401 aaagaccagg tgctggtcaa gctgaacccc accaacgcca atgcccagct gggcattgtt
     2461 caggcgcagt ttctggcggc gcaggccatt gccgaccggc tgagcgccga gcgcgaaggc
     2521 aagacgacgg tgactttctc gccggtgctg accgaaaaat ttggcaatga cagccgcgcc
     2581 aaagacgcca tggccctgca agcgcaattg tttgcctcgc gccgtgccgc gctgcaagcc
     2641 gaactgggca tcctgaacga aaacagccag ggcatgcagg aacagctcaa gggcctgcaa
     2701 tcgctgaaag actcgcgcga acaacagctg aaattcatcc aggaagagct caagggcgtg
     2761 cgtgacatgg ctgcgcaagg ctatgtgccg cgcaaccgca tgctgcaact ggagcgcaat
     2821 gtggccgaaa tcagcggcca gctggccagc aatatcggcg acatcggccg cagcaagaac
     2881 gcgctgtccg aaatcaaact gcgcatgttg cagcgtcaga aggaatacca aaaagaagtc
     2941 gaaacccagc tgagcgatgc gcaaaaagaa gcgcgcgccc agggcgatcg tctggccgcg
     3001 ctggaatatg aactgtccaa cacccagatc aaatcgccga ttgacggcac cgtggtgggc
     3061 gtgaatgtgc ataccgtggg tggcgtgatt cagccgggct tccacatcat ggacgtggtg
     3121 ccgctgaacg agccgatgat tgtcgaagca cgcatccaga ccaacctgat cgacaaggtg
     3181 catcccggcc tgccggtgga cctgaccttc ccggccttca gccatgcgca aacgccgaat
     3241 atccccggcg tggtggacac cgtgggcgcg gatgcgctga ccgatgaagt cacccgcatg
     3301 ccgtactacc cgatgcaggt gaaagtgacc ccggaaggca tgaaaatcct gggcaagctg
     3361 caaattcgcc ctggcatgcc ggccaccgtg gtggtgaaaa cgggtgaacg caccatgctc
     3421 aactacctgc tcaagccgct gatcgaccgc gtcagcgggg cgttcaaaga ggaatgatcg
     3481 tgcgccttcg caagctgacc ctgctgactg cgctggcgct gagcctgaac cccctgctgc
     3541 cggcctcggc gctggggttc atggaagcct accgcgcagc attgcagaat gacccgacct
     3601 accaggccgc catcgcagaa aacgaagccg gccagcaaaa ccgcgccatg ggccgcgcag
     3661 gcttgttgcc gcagatcagc gccaatgcct cgcgcgccag agtgcgcggc gagcgcgaat
     3721 acaatacgca aaccctgtcg ggggtgcgta atgtgattga accgctgagc tatttcagca
     3781 cctcgcgcac ggtgcaagtg cgccagtcgg tgctgaatat ggcggcgatt gccaacttcc
     3841 gccagggccg cgcccgtgct gagtacagca cggaagtgtt ccgaggcaag caaaacgacc
     3901 tggccttgcg cctgtcgtcg gcgtatttcc aggccttgct gtcgcagcac gccattgatc
     3961 tggcccaggc caaggtgcaa tccatcgagc aacagctgaa aaccgccgaa gtgcagttca
     4021 aggcaggcga tggcaccgtc accgatgtgg acgaggcccg cgcccgccgt gatctggcgc
     4081 aagcccagct gatcgaggca cgtaaccagc tgaccgtggc catgcgcagc ctgcaagagc
     4141 tggtgggcga caagccggtg tcgctggccc agctgaaggc cgattttgcc aaacagctca
     4201 agcccgagct ggaaaccctg gacacctggc tggatcgcgc actggaaacc agcgccgaca
     4261 tcgccgccca gcgccgggcg ctggaagtgg ccgaacgcga agtggacaaa aaccgcgccg
     4321 gccatctgcc cacggtggat gtgtacgcca gctctggtcg ctctcagtcg gacagttttt
     4381 ccagcatcaa ccaggaatac cgcacccgcc agttgggcgt acaggtgaat atcccgattt
     4441 tctctggcgg ttacaccaac gcggccacca cgcaagccat cgccaaccgc gagcgcgcgc
     4501 gcaacgagct ggaagccacg gtgaacaaaa cccatattga agtcaccaag cagtattccg
     4561 gcacggtgtc tggcctggcc aaggtgcagg cgctggagct ggcggtgaaa tccagcgagc
     4621 aggcactgga atccaccaaa atgggcttca aggcaggcat gcgcagcaat gtggacatcc
     4681 tcaacgccga agagcagctg tatacctccc gtcgtgatct ggccgaggcc aagtatgtct
     4741 acctgctcag ccgcctgcgg ctgaaagccg ccgccggcca gctgtcggaa gcggacatgg
     4801 gcgaagtgga cagctatttt ggcgcggaaa aaccggtgca cgaaaaactg gtgcaggcac
     4861 gccgtagcgc caacggcggt tgatgccgca aaatggggtg atgatgcaca gcaccggccc
     4921 ggccttgccg ggccggtttt tcatcgggag aacaccatgc aaattgtgga acacgcgctc
     4981 aacggcgtgc tgctgatcga accccgcgtg tttggtgacg aacgcgggtt tttctttgaa
     5041 agttacaacc agcaggcgtt tgccgccgcc tgtggccaga ccgtgccctt tgtgcaggac
     5101 aaccactccc gctccagcgc caatgtgctg cgcggcctgc actaccagat cgaacacgcc
     5161 cagggcaaac tggtgcgggt gatccacggc gaagtgttcg acgtggcggt ggatctgcgc
     5221 cgcagctcgc ccagctttgg ccagtggacc ggccattatc tgtccgccgc caacaagcgc
     5281 cagctgtggg tgccggccgg ttttgcccat ggttttctgg tggtcagcga aaccgccgag
     5341 ctgctgtaca aaaccactga tttttaccgg cccgagcacg agcgctgcat cgcctggaac
     5401 gacccgcagc tgaatatccg ctggccgctg ccagaaggca ttgcgccgat tgtctccgcc
     5461 aaggaccgcg tcggcctggc gtttgccgat gcgccgagct acccatgagc gcccgcccca
     5521 tcctgctgat tggcgcccac ggccagctgg gccgcgagct ggcgcgcagc ctggcggtgc
     5581 tgggcccgct gcatgcgctg ggccgcgccg agcttgacct caccgacgcg gacgccatcg
     5641 cccgtgtggt ggacgacgtc cgccccggcc tgatcgtcaa cgccgccgcc tacaccgcgg
     5701 tggaccgcgc cgaaagcgag cccgagctgg ccttcgccat caacgcccgc gcgcccggcc
     5761 tgctggccga agccgccgcc cggctggatg gcggcctggt gcactattcc accgattatg
     5821 tgttctccgg cgagggcgcc accccctggc gagaaagcga cgccactgcg ccggtgaatg
     5881 tgtacggcgc cagcaagctg gctggtgagc aagccgtgct ggccagtggc gccgacgcgc
     5941 tgattttgcg caccagctgg gtgtacggcg gccccggaca gaactttgtc aacaccatgc
     6001 tgcgtctggc cggcgagcgc gaacggctga ccgtggtgga cgaccagatt ggcgcgccca
     6061 ccgccagccg ttttctggcc gacgccaccc tggctgcgtt ggcgcaagac tggcgcgccc
     6121 gcgccgggct gtatcacctc agcgccagcg gcgacaccag ctggtgcggc tttgcccgcg
     6181 ccattgtcga gcaggcgctg gcgcacggcc aggcgctgaa gctgagcgcc gccgacatcg
     6241 cccccatccc cagcagcagc taccccaccc cggcgcgccg cccggccaat tcccgtttgg
     6301 attgctcggc gtttgagcag gcgtttgccg tgcatcgccc gcactggcgc gcgcagctgg
     6361 ccgcgcattt tgccagtcgg ggctgaaccg ctggcaatag cggtcagtca gatgggtttt
     6421 tctgaaagcg tgtgatgatc tggcattggc aattcgaccc catcgccctg catgtgggcc
     6481 cgctgcaatt gcactggtac ggcgtgctgt tcgccctggc ctttgtgctg ggccagcagc
     6541 tgctggcctg gtgttaccgc cgcgaaggct acgacagcca ggcgctggac agcctgatgc
     6601 cgtgggcgct gattggcacg gtgctgggcg cgcggctggc gcattgcctg atctacgacc
     6661 cggcctatta cctcagccag ccgctggcca tcctgcgggt gtgggaaggc gggctggcca
     6721 gccacggcgg cgcgctgggg ctgatgctgg ccctgtggct atacagccgc cgccatccgc
     6781 agccgggttt tgcctggctg ctcgaccgca tcgccctgcc ggcggcgctg ggcggcgcgc
     6841 tggtgcgctg cgccaatttt ctgaactcgg aaatcctcgg ccgcccaacc ggcggcgact
     6901 ggggcgtggt gtttgccgcc atcgacccgc tgccgcgcca cccggtgcag ctgtacgaag
     6961 ccggcgccta tctgctgatc gccgccctgc tgtggacggt gtaccgccgc cacggccgcg
     7021 ccacccccag cggcctgctg gccgggctgt ttttgctgac ggtgtttgcc gcccgcgcgc
     7081 tgctggaaac cctgaaaacc ccgcaagcgg cgtatgaaac cggctttgcc gtcagcgtcg
     7141 gccagtggct gagcctgcca ctgatggcac tgggcctgtg gctggtgtgg cgcgccaagc
     7201 gccgtgcctg atccattgaa tgaactcagc ccggccccgg ccagatttgg cgccacggcg
     7261 gctccggctg aagcaaatcc tccagcgccg ccaaaaacac ctgcaccttg ggcagttgca
     7321 cccggctggg ggtgtagcag gcgtataaat gctggccaaa gcgcacttgc ggctggcagt
     7381 cgggcaacac ctggcgcagg cggccgtcgg ccagcgccgg cgcgcacaga tacaccggca
     7441 agatgctcaa gccctggccg gccagggtgg cctgatacag gcagtcgata ttgttgtact
     7501 gcacccgccc gcgcaccggc gcgctccatt cttcaccgcg cgcatcgcgc agccgccagt
     7561 ggccgtcatc ggtgagcaga tagctgaaac actgatgctg ggccagctgc aaagggtgct
     7621 ccggcgtccc gtggcgggcc agataatccg gcgcggcgca aatcacccgc tccatctgca
     7681 agagcggctt ggccaccgcg tcggccggcg gggtttgcgt ttgccgcagc gccaggtcga
     7741 tgcggtcgtc caccaaatcc accatctggt cggtgaggat cagctcgcac tccagctcgg
     7801 ggtggcgttc ggtcagcgcc ggcagcagcg gggcgatgta ttcgcggcca aaagtcatgg
     7861 tggcgctgat gcgcagcagg ccgcgcggcg cgctgcccag atggctgagc gcgcgctgcg
     7921 cgcagtccac cgccgccacg gcggccagcg cgtgcggata aaacgcctcg ccggccggcg
     7981 tgggccgcag gcgacgggtg gagcgctcga acaggcgcgc gcccagcgcg gcctccagct
     8041 ggccaatctg ccggctgacc tgggcgcggc tgcaccccag ccgccgcgca gcgccggcca
     8101 tgctgccgct ttccaccacc ttgataaagc tgtcccagct gtccagccat gcgctcattg
     8161 tcacgctttc attgacggta tgccgccaat tatcggctag atcgtcacca gttgggcgac
     8221 tatagtggcg ctttggcggt tgcctgttct tt
//
LOCUS       DAOPYX010000122         8239 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_10383_length_8239_cov_10.454912, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000122 DAOPYX010000000
VERSION     DAOPYX010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 8239)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8239)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8239
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10383_length_8239_cov_10.454912"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(169..828)
                     /gene="cmk"
                     /locus_tag="PKN55_07590"
     CDS             complement(169..828)
                     /gene="cmk"
                     /locus_tag="PKN55_07590"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003774569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HNX71143.1"
                     /translation="MTIPVITLDGPSASGKGTVAARVAASLGFHYLDSGALYRLMALY
                     AMRNQIALDHEARLAMAAVRLPATFAEGVVKLEGEDVSDAIRAEEVGIGASKVAVLPA
                     LRAALLQRQRDFRQAPGLVTDGRDMGSVVFPDATVKVFLTASAAERARRRHKQLIDKG
                     EAANILQIQQDIEERDARDAARPVAPLKAEADAHMLDTTDLTVDQAVAQVLAWWRNTA
                     N"
     gene            971..2149
                     /locus_tag="PKN55_07595"
     CDS             971..2149
                     /locus_tag="PKN55_07595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX71144.1"
                     /translation="MSEYQYYEFVAVDQPLTSKQQAELRKHSSRATITASSFINEYHW
                     GSFKGDATAWMQQYFDAHVYSASWGSCCLLLRAPANALDLSMLDACQSCDNREWQDDQ
                     DAFNRSRYGDNYLLAWHFSDDTGENERFWSEDDGPDWMLRLLPLRAELLRGDARPLYL
                     GWLARLCAGQLDDEHVEPPVPAGLTELTPAQQALAEFLVLDVDLLAAAASASSELEPV
                     STADHDRWLAAQSAHALQASVRLLLEGRAPEAERSVRQRFRAWQAAQRPEQAPAPRRQ
                     VAEIQHIAQQIRQQRQQAERQAAAEAQARQQAARAIQLKALAADAARVWKDIDTQLNR
                     GVASGYDQALQLTLALAEAMQHVERHAEFQHALNQLLTVHGKRRAWMARLEKTKLLAR
                     "
     gene            2316..>3523
                     /gene="aroA"
                     /locus_tag="PKN55_07600"
     CDS             2316..>3523
                     /gene="aroA"
                     /locus_tag="PKN55_07600"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019921779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HNX71145.1"
                     /translation="MEHLDLAPIARAEGSIVLPGSKSISNRTLLLAALAEGVTEVLGL
                     LDSDDVTVMLAALDQLGVRIQRHGDSRDFTVSGVAGPFPVKSGNFFLGNAGTAFRPLT
                     AALALSGGDYQLSGVPRMHERPIGDLIDALRGLGAQVAYTGNPGYPPLTLSPAQGSGA
                     DQVAVRGDVSSQFLTALLMALPLTGRRVTVQVIGELISKPYIDITLNLMARFGVTVER
                     DGWAAFTVDGSQRYHSPGVIHVEGDASSASYFLAAGALGRGPVRVEGVGSGSIQGDVR
                     FADALAAIGADIRWGDNWIEARGTGRVKAFDADFNHIPDAAMTLAVAALAADGRSVLR
                     NIGSWRVKETDRIAAMAAELRKVGAVVEEGEDWLAITPPAQLTPNAAIDTYDDHRMAM
                     CFSLVALLGAP"
     assembly_gap    3524..3623
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3757..4314
                     /locus_tag="PKN55_07605"
     CDS             3757..4314
                     /locus_tag="PKN55_07605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008954010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1003 domain-containing protein"
                     /protein_id="HNX71146.1"
                     /translation="MQIRDEKHIRYLAEHLLHTGIGQLGRRDIHALRRIALRLQAQYD
                     ASVPEEGMTFGERLADRVAEFGGSWTFISLFASLLVGWALLNTEILGQNAFDPYPYVF
                     LNLVLSMLAAVQAPIIMMSQNRQAARDRMTMIRDFEVNLKADAAISELNQRLDHLAWQ
                     VLADLDQIKRAQKIDPTAPTPPAAG"
     gene            complement(4408..6885)
                     /locus_tag="PKN55_07610"
     CDS             complement(4408..6885)
                     /locus_tag="PKN55_07610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011137280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HNX71147.1"
                     /translation="MPGFERMRLALRMMRRELRAGELTVLLAALLVAVAAMSSVGFFT
                     NRVDRALHLQASQLLAADVVINSDQDNLARWQGQLGGLRHTQTLTFPSMTLANGQSQL
                     ATFKAVSPGYPLRGEVTVSQHGQTRSGQLIPAPGTVWADARLLNKLKLREGDWLEVGA
                     SRLQVAGVLVREPDAAMDIYNFIPRLMLHANDVAATGLIQTGSRARWRLLLAGAPAEV
                     EAFRQQASAQLARGERIENVEEARPEIRTALERAKRVLGLTALLAVALSAVAVALACR
                     RYVNRHRQAVAVMRCLGASQGDMLWLHLSQFFALALLAGLAGCALGWLTQELVLAQLA
                     SLLGQSLPPAGWQPWAQGLAVGATLLFGFAVPPLLALRRAPTLAVLRDAPPDDAAARG
                     SYLLGGATLLALMGWQAGEWPLALAVALGFAGFAALAALLAWMLLALLRRLPGSGQVG
                     WRYGLANLARRRALSITQMVALASGLMALLILTVVHGDLLAAWQRNVPADAPNRFVIN
                     IQPPQREPVRELLRSQGLPAPELAPMVRARLLSINDRQVRPEQYPDERAQRLAEREFN
                     LSWAEQLPADNRISAGAAWDPSKPGFSVEEGIADTLGIRVGDVLRFDLAGNELHAPVT
                     SLRKVQWDTFKVNFFVYASPAMLANQPASYITSFHLPTTQAAVVDLLVAKFPNLTVLD
                     VGLILAEVRNMVGRLVEAMRGVFGFSLLAGLVVLWTATVSTQDERARDVALLRTLGAS
                     RRQVVGVVLGELLWLGGLAGLLAALGALALSAIVSARLLNLPVEANPWLLLWGPLAGA
                     VAVTLAGWPTGRQIVRTPPLDTLRRLG"
     gene            complement(6872..7573)
                     /locus_tag="PKN55_07615"
     CDS             complement(6872..7573)
                     /locus_tag="PKN55_07615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012697665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNX71148.1"
                     /translation="MSAPSLTPILQAVDVARQVVYGGDTLTILRGVNMTVATGETVAI
                     VGSSGSGKSTLLGILAGLDHASNGQVLLFGKSLSHLSEDERAKLRQRLVGFVFQSFQL
                     LPALTALENVMLPLELAGLRGAREQAAAMLARVGLAHRLGHYPRHLSGGEQQRVALAR
                     AFVGEPKLLFADEPTGNLDADTGAQVIELLFTLNREQDTTLVLVTHDASLAARCERVL
                     RLEQGKLTEARHAGV"
     gene            7572..8165
                     /locus_tag="PKN55_07620"
     CDS             7572..8165
                     /locus_tag="PKN55_07620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018151146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylesterase"
                     /protein_id="HNX71149.1"
                     /translation="MKRMILLCMAMLLSAPGWAATIMVFGDSLSAGYGLRPGEGWVDL
                     LRQALPAHQVVNASQSGETTAGGLSRLPAALARHKPDIVILELGGNDGLRGLPPDDMR
                     ANLEQMVAMSAQSKARVLLVGMALPPNYGQAYGRAFQMVYSDLAKQRKLPFVPLLVEG
                     FATDRTRFQADGIHPNASAQGQMRDSVLKVLRGMVKN"
BASE COUNT         1381 a         2772 c         2666 g         1320 t
ORIGIN      
        1 cgaggctttc ctcgaacagc gcggcaaagc tttcttgggt ggaggtagtc attaaaggaa
       61 ctcttgcatg cacgctagcc agcgtgcggg gttggttaaa cacaaccgcg cccggttagg
      121 ccggacgcgg cgatgaaaaa ttgctttgaa ttcaaatggt ttggcggctc agttggctgt
      181 gttgcgccac caggcgagca cctgcgccac cgcttgatcc accgtcagat cggtggtgtc
      241 cagcatatgg gcgtcggctt ctgccttcag cggggccacc gggcgggcgg catcgcgcgc
      301 gtcccgttcc tcaatgtcct gctgaatctg aaggatgtta gcagcttccc ccttgtcgat
      361 caactgctta tggcggcggc gcgcgcgttc ggcggcgctg gcggtgagga acaccttcac
      421 cgtggcgtcg ggaaacacca ccgagcccat gtcgcggccg tcggtcacca gccccggcgc
      481 ctggcgaaag tcgcgctggc gctgcaacaa ggcggcgcgc agcgccggca gcaccgccac
      541 tttggacgcg ccgatgccca cttcttcggc gcggatggcg tcgctgacat cctcgccctc
      601 cagcttgacc acgccctcgg caaaggtggc cggcagccgc accgccgcca tggccagccg
      661 cgcctcgtga tccagcgcaa tctggttgcg catggcgtac agcgccatca agcggtacag
      721 cgcgcccgaa tccagataat gaaaacccag gctggccgcc acccgcgccg ccaccgtgcc
      781 cttgccggag gccgacgggc cgtccagggt aatgaccgga attgtcatgc tgcacatcca
      841 aatgattcaa agccgacatt ttaccggggc ggacagatga cggatagcgg gtgccgggcc
      901 ggcaatccgc aagcatgccc cgcaacaagg taaactgtgc gcaggacaaa cgcgggcgcc
      961 agatctctcc atgagcgaat accaatacta tgaatttgtc gcggtcgatc aaccattgac
     1021 aagcaagcag caagctgagc tgcgcaagca ctccagccgg gcgaccatta ccgccagcag
     1081 cttcatcaac gaataccact ggggcagctt caaaggcgac gccaccgcat ggatgcaaca
     1141 gtactttgat gcccatgtct attcggccag ctggggcagt tgctgtctgc tgttgcgggc
     1201 gccggccaac gcgctggacc tgtcgatgct ggacgcctgc cagtcatgcg acaaccgcga
     1261 gtggcaggac gaccaggatg cattcaaccg cagccgctac ggagacaatt atctgctggc
     1321 atggcatttt agcgatgaca cgggcgagaa cgagcgcttc tggagcgaag acgatggccc
     1381 cgactggatg ctccgtctgc tgcccttgcg cgccgaactg ttgcgtggcg atgcccgtcc
     1441 gctttatctg ggctggctgg cgcgcttgtg cgctgggcaa ctggatgatg agcatgtgga
     1501 gccgccagtg ccggccgggc tgactgaact caccccggca cagcaggcgc tggcggaatt
     1561 tttggtgctg gacgtggacc tgctggctgc cgccgccagc gccagctccg agctggagcc
     1621 agtctccacc gctgaccacg atcgctggct ggctgctcag tcagcgcacg ccttgcaggc
     1681 cagcgtgcgc ttgctgctgg aaggccgcgc tcccgaagcc gaacgtagcg ttcgccagcg
     1741 ctttcgggct tggcaggcgg cccagcgccc tgagcaagcc cctgcgccgc gccgtcaggt
     1801 ggctgagatc cagcatattg cgcaacagat ccgccagcag cggcagcagg ctgaacgtca
     1861 ggcggccgcc gaggcgcaag cccggcaaca agccgctcgc gccatccaac tgaaggcatt
     1921 ggccgccgac gctgcgcggg tgtggaagga cattgacacc cagctgaacc gtggcgtcgc
     1981 ctcaggctac gatcaggcgc tacagctgac gctcgcactg gcggaagcga tgcagcatgt
     2041 cgagcgccac gccgagtttc agcacgcgct gaaccagttg ttgaccgtgc atggcaaacg
     2101 ccgcgcctgg atggctcgcc tggaaaaaac caagctgctg gcgcgctgaa ttcatgccta
     2161 tctcgtgcgc gcgctgcata aaacccgcct gaccctgcca agcgacgcac tttgtggtag
     2221 cgtgctggct gcatacgcca gttccagaca catgttgcga cctcgtggct tggcctggcg
     2281 gcgtgcgctg accctttgac cttgccggac ttcgcatgga acacctcgac ctcgccccca
     2341 tcgcccgcgc cgaaggcagc atcgtgctgc ccggctccaa gagcatttcc aaccgcaccc
     2401 tgctgctggc cgcgctggcc gaaggcgtca ccgaggtgct cggcctgctg gattctgatg
     2461 acgtcaccgt catgctggcc gcactggacc agctgggcgt gcgcatccag cgccatggcg
     2521 acagccgcga ctttaccgtc agcggcgtgg ccgggccgtt tccggtgaaa tccggcaatt
     2581 tctttctggg caacgccggc acggcgtttc gcccgctgac cgccgcgctg gccttgtctg
     2641 gcggcgacta ccaattgtcg ggcgtgccgc gcatgcacga gcgcccgatt ggcgacctga
     2701 tcgacgcgct gcgcggcctg ggcgcgcaag tggcctacac cggcaaccct ggttacccgc
     2761 cgctgacgct cagcccggcc cagggctctg gcgctgacca ggtggcggtg cgcggcgatg
     2821 tgtccagcca gtttctcacc gccttgttga tggcgctgcc gctgaccggg cggcgcgtca
     2881 ccgtgcaggt gattggcgag ctgatctcca aaccgtatat cgacatcacc ctgaacctga
     2941 tggcgcgctt tggcgtgacg gttgagcgtg acggctgggc ggcgttcacc gtggatggca
     3001 gccagcgtta ccacagcccc ggcgtgatcc atgtggaagg cgacgcctcc agcgcctcgt
     3061 attttctggc ggccggcgcg cttgggcgcg gcccggtgcg ggtggagggc gtgggcagcg
     3121 gcagcatcca gggcgatgtg cgctttgccg acgcgctggc cgccatcggc gccgacatcc
     3181 gctggggcga caactggatt gaggcgcgcg gaaccggccg ggtgaaggcg tttgacgcgg
     3241 acttcaacca catccccgac gccgccatga ccctggccgt ggcggcgctg gccgccgatg
     3301 gccgctcggt gttgcgcaat atcggcagct ggcgggtgaa ggaaaccgac cgcatcgccg
     3361 ccatggctgc cgagctgcgc aaagtcggcg ccgtggtgga agaaggcgaa gactggctgg
     3421 ccatcacccc gccagcgcaa ctgacgccga acgccgccat cgacacctac gacgaccacc
     3481 gcatggccat gtgcttttcg ctggtggcgc tgctgggcgc gccnnnnnnn nnnnnnnnnn
     3541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3601 nnnnnnnnnn nnnnnnnnnn nnngcttgcc tttgcgggtt caccacgcta ctgtccgcgt
     3661 cacccgccgg cttggctggg tactgcgcct gcgttcggcc ctgctctgcg atggccaagc
     3721 cgttgatttt ctgtccccat atctggaggg cgctatatgc aaatccgtga cgaaaaacac
     3781 atccgctacc ttgccgaaca cctgctgcac accggcatcg gtcaactggg ccgccgtgat
     3841 attcacgccc tgcgtcgtat tgccctgcgt ttgcaggcgc agtacgacgc cagcgtgcca
     3901 gaagaaggca tgacctttgg cgagcggctg gccgaccggg tggccgagtt tggcggctcg
     3961 tggaccttca tcagcctgtt tgccagcttg ctggtgggct gggcgctgct gaataccgaa
     4021 atccttggtc agaatgcctt tgacccgtat ccctatgtgt ttctgaatct ggtgctgtcc
     4081 atgctggcgg cggtgcaggc gccgatcatc atgatgtcgc aaaaccgtca ggcggcgcgc
     4141 gaccgcatga ccatgatccg cgattttgaa gtgaacctca aagccgatgc cgccatcagc
     4201 gagctgaacc agcggctgga tcatctggcc tggcaggtgc tggccgacct tgaccagatc
     4261 aagcgcgccc agaagatcga ccccaccgca cctacaccac ccgcagccgg gtaagtgtta
     4321 tcttgctgcg ccgccggctg cgtagccgca tccacgcacc agctgcatgt atgccatcgg
     4381 ccacgttagc gcgtggccga tggcacgtca gcccagccgc cgcagggtat ccagcggcgg
     4441 ggtgcgcaca atctggcggc cggtgggcca gccggccagg gtgacggcca cggcgccggc
     4501 cagcgggccc cacagcagca gccaggggtt ggcttctact ggcaggttca gcagccgggc
     4561 gctgacgatg gcggacagcg ccagcgcgcc cagcgccgcc agcaggccgg ccaggccgcc
     4621 cagccagagc aattcgccca gcaccacgcc caccacctgc cggcgcgaag cgcccagggt
     4681 gcgcaatagc gccacgtcgc gggcgcgctc gtcctgggtc gagacggtgg cggtccacag
     4741 caccaccagc ccggccagca ggctaaagcc aaacaccccg cgcatggctt ccaccaggcg
     4801 gccgaccata ttgcgcactt ccgccagaat caggccgacg tccagcacgg tgaggttggg
     4861 gaatttcgcc accagcaagt ccaccacggc cgcctgggtg gtcggcaggt gaaagctggt
     4921 gatatagctg gccggttgat tggccagcat cgccgggctg gcgtaaacaa agaaattcac
     4981 cttgaaggtg tcccactgca ccttgcgcag gctggtgacc ggcgcgtgca gctcgttgcc
     5041 ggccaggtca aagcgcagca cgtcgcccac gcgaatgccc agggtgtcgg caatgccttc
     5101 ttcgacagaa aaccccggct tgctgggatc ccacgccgcg ccggcgctga tgcggttatc
     5161 cgccggcagt tgctcggccc aggacaggtt gaactcgcgt tcggccaggc gctgggcgcg
     5221 ttcgtccggg tattgctcgg ggcgcacctg gcggtcgttg atcgacagca gccgcgcgcg
     5281 caccatcggc gccagctcgg gcgctggcag gccctggctg cgcagcagct cgcgcaccgg
     5341 ctcgcgttgc ggcggctgga tattgatgac aaagcggttg ggcgcgtcgg ccggcacatt
     5401 gcgctgccag gcggccagca ggtcgccgtg caccacggtg agaatcagca aggccatcag
     5461 cccgctggcc agcgccacca tctgggtgat ggacagcgcg cgccgccggg ccaggttagc
     5521 caggccatag cgccagccca cctggcccga tccgggcagc cggcgcagca gagccaacag
     5581 catccaggcc agcagcgccg ccagcgcggc aaagccggca aagcccagcg ccaccgccag
     5641 cgccagcggc cattcgccgg cctgccagcc catcagcgcc aacagcgtgg cgccgcccag
     5701 cagatagctg ccgcgcgcgg cggcgtcgtc cggcggggcg tcgcgcagca cggccagcgt
     5761 gggcgcgcgg cgcagcgcca gcagcggcgg cacggcaaag ccaaacagca aggtggcgcc
     5821 caccgccagg ccttgcgccc acggctgcca gccggccggc ggcaggcttt gccccagcag
     5881 gctggccagt tgggccagca ccagctcttg cgtcagccag cccagcgcgc agccggccag
     5941 cccggccagc agcgccaggg caaaaaactg gctgaggtgc agccacagca tatcgccctg
     6001 gctggcgccc aggcagcgca tcaccgccac cgcctgccga tggcgattga cataccgccg
     6061 gcaggccagc gccaccgcca cggcggacag cgccaccgcc agcagcgcgg tcaggcccag
     6121 cacgcgcttg gcgcgctcca gcgcggtgcg gatttccggg cgggcttctt ccacgttttc
     6181 gatgcgctcg ccgcgcgcca gctgggcgct ggcctgctga cgaaacgcct cgacctcggc
     6241 cggcgcgccg gccagcagca gccgccagcg cgcgcggctg ccggtctgga tcaggccggt
     6301 ggcggctacg tcgttggcgt gcagcatcag ccggggaata aagttataaa tatccatcgc
     6361 cgcgtctggc tcgcgcacca gcacgccggc cacttgcagc cggctggcgc cgacttccag
     6421 ccagtcgcct tcgcgcagct tgagcttgtt cagcaagcgg gcgtcggccc acaccgtgcc
     6481 cggcgccgga atcagctggc cgctgcgcgt ttgcccatgc tggctgacgg tgacttcgcc
     6541 gcgcagcgga taaccggggc tgaccgcctt gaaggtggcc agctggcttt ggccattggc
     6601 cagggtcatg ctgggaaagg tcagcgtctg cgtgtggcgc agcccgccca gctggccttg
     6661 ccagcgggcc agattgtcct ggtcgctatt gatcaccaca tccgccgcca gcagctggct
     6721 ggcttgcaga tgcagggcgc gatcgacccg gttggtgaaa aaccccacgc tgctcatcgc
     6781 cgccaccgcc accagcaggg cggccagcag cacggtgagc tcgcccgcgc gcagttcgcg
     6841 gcgcatcatg cgcagcgcca gccgcatgcg ttcaaacccc ggcatggcgc gcctccgtca
     6901 gtttgccttg ctccagccgc agcacccgct cgcagcgcgc cgccaggctg gcgtcgtggg
     6961 tgaccagcac cagggtggtg tcttgttcgc ggttgagggt gaacaagagt tcgatcacct
     7021 gcgcgccggt gtcggcgtcc agattgccgg tgggctcgtc ggcaaacagc agcttgggtt
     7081 cgccgacaaa agcccgcgcc agcgccaccc gctgctgttc gccgccagac aaatggcgcg
     7141 gataatgccc cagccggtgc gccaggccca cccgcgccag catggcggcg gcctgctcgc
     7201 gcgcgccgcg cagcccggcc agctccagcg gcagcatgac gttttccagc gcggtcagcg
     7261 ccggcagcag ctgaaacgac tgaaacacaa aacccaccag ccgctggcgc agcttggcgc
     7321 gctcgtcctc gctcaggtgc gacagacttt tgccaaacag cagcacctgg ccattgctgg
     7381 cgtggtccag cccggccaga atgcccagca gggtggattt gcccgagccg gaacttccca
     7441 caatcgccac cgtctcgccg gtggcgacgg tcatattgac tccgcgcaga atggtgagcg
     7501 tatcgccgcc atagaccact tgccgggcga catcgacggc ttgcaaaatc ggcgtcaaag
     7561 aaggtgcaga catgaaaaga atgatcctgt tgtgtatggc catgctgttg agcgcgcccg
     7621 gctgggcggc caccatcatg gtgtttggcg actcgttgtc cgccggctat ggcttgcgcc
     7681 ccggcgaagg ctgggtggat ttgctgcgcc aggcgctgcc cgcccatcag gtggtcaacg
     7741 ccagccagtc gggcgaaacc acggccggcg gcctgtcgcg cctgccggcg gcgctggcgc
     7801 ggcacaagcc cgatatcgtc attctggagc tgggcggcaa cgacggcctg cgcggcctgc
     7861 cgccggatga tatgcgcgcc aatctggagc agatggtggc catgtcggca caaagcaagg
     7921 cccgcgtgct gctggtgggc atggcgctgc cgcccaacta cggccaggcg tatggccggg
     7981 ctttccagat ggtgtattcc gacttggcca aacagcgaaa gttgccgttt gtgccgctct
     8041 tggtggaagg ctttgccacc gaccgcacgc ggtttcaggc ggatggcatt catcccaacg
     8101 ccagcgcgca ggggcagatg cgggacagtg tgctgaaggt gttgcgcggg atggtaaaaa
     8161 actaagagcg gctaacaaaa cccgcctggc gttgttgccc gaccttgccg tactacccgt
     8221 actgtctacg gtcgtgcgc
//
LOCUS       DAOPYX010000123         8230 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_10395_length_8230_cov_13.202569, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000123 DAOPYX010000000
VERSION     DAOPYX010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 8230)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8230)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8230
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10395_length_8230_cov_13.202569"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2348)
                     /gene="gyrB"
                     /locus_tag="PKN55_07625"
     CDS             complement(<1..2348)
                     /gene="gyrB"
                     /locus_tag="PKN55_07625"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012698682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HNX71150.1"
                     /translation="MSEQEYDSSSIKVLKGLDAVRKRPGMYIGDTQDGTGLHHMVFEV
                     LDNAIDEALAGHADTIKVIIHPDESISVEDNGRGIPTDIHPEEGRSAAEVIMTVLHAG
                     GKFDSNSYKISGGLHGVGVSVVNALSDWLKLKIWRNGEVHEMEFNHGEPVAPLVKTGQ
                     TSHRGTKVTFHASEATFGKVEFHFDILAKRIRELSFLNDGVAITLIDERNGKEENFAF
                     SGGVAGFVQYMNRNKNPLHPKVFYGIGEKDGMTVEVAMQWNDSYQEAVQCFTNNIPQR
                     DGGSHLTALRQVMTRTLNGYIEQSEIAKKAKVDTTGDDMREGLACVLSVKLPDPKFSS
                     QTKDKLVSSEIGPVVNEVISESLKTFLLENPADAKSICSKIVDAARAREAARKAREIT
                     RRKGIMDGLGLPGKLADCQEKDPAMSELYLVEGDSAGGSAKQGRDRKFQAILPLKGKI
                     LNVERARFDKLIASQEIATLITALGTSIGKDDYNPEKLRYHRIIIMTDADVDGAHIRT
                     LLLTFFYRQMPELVERGHIYIAQPPLYKVKHGKTEQYLKDEHELNQYLLKLALSNAQL
                     IPGDGMTPLTGDTLGEIARQYLLAQAVIERESRLIDPSVLQALLKTDPVDLAETASAE
                     AALAALRALLPADSIDLSLTADEKNGGYLLKIIRKLHGNVMVSYLDQSFLQSGDYTQI
                     KATGALLKGLLHSGAAIKRGEQGRQINEFGEALNWLLNEVRRNISIQRYKGLGEMNPE
                     QLWETTMDPTVRRLLKVRIEDAMSADDVFTTLMGDQVEPRRH"
     gene            complement(2440..3546)
                     /gene="dnaN"
                     /locus_tag="PKN55_07630"
     CDS             complement(2440..3546)
                     /gene="dnaN"
                     /locus_tag="PKN55_07630"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HNX71151.1"
                     /translation="MHILQAERDALLKPLNAVTGIVERRHTLPILANVLIEKRGNALS
                     FLATDLEIQITTASQGEVAGEDFRLTTSAKKLQDILRALPDGATVTLDQQDAKLTLKA
                     GKSRFNLQTLPAEDFPLLSVGAEPLSKLTVPQKALKALLAKVQYAMAVQDIRYYLNGM
                     LFIAEGNQLKLVATDGHRLAFACMTLDAEVARAEAILPRKTIVELAKLLADSDDTVSI
                     ELLPNQARFAFGQSVIVSKVVDGKFPDYNRVIPLDNDKIFLINRQPLLQALQRAAILA
                     NEKFRGVRLTLAAGSLSIQCTNSEQEEAEEELEIAYQGAPLEIGFNINYLTDVLTNLQ
                     VEQLQLAFGDANRSALFTLPEQADFKYIVMPMRI"
     gene            complement(3616..5019)
                     /gene="dnaA"
                     /locus_tag="PKN55_07635"
     CDS             complement(3616..5019)
                     /gene="dnaA"
                     /locus_tag="PKN55_07635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014085475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HNX71152.1"
                     /translation="MTDLHTFWPTCLERLEAELSAQQFTTWIKPLRCEPGAEGWVLLA
                     PNRFVLQFIKDRFLARIEALASEVLGEPALLELKIGAPVKVEPPVMTSSSASPSASSG
                     AQPPSSPTSPSARPAAVQAIGGGHESTRLNPSFTFESLVTGKGNQLARAAAMQIADNP
                     GDPAYNPLFVYGGVGLGKTHLVQAIGNHVFQKNPKARIRYIHAERYVADIMRAYQHKA
                     FDEFKRYYHSLDLLLIDDIQFFAGKNRTMEEFFYAFNALIEGGKQVIMTCDTFPKQIE
                     GMDERLISRFSWGLTVEIQPPELEMRVAILMKKAEADNLKLDNNVAFFIAQNVRSNVR
                     ELEGALKRVVAYSRFTNQAIELELVKEALKDILAAGNRVISIETIQKTVAEYYKIKLA
                     DMHSKKRSRDIARPRQIAMALAKELTQLSLPNIGDAFGGRDHTTVLHACKTIAEMRTT
                     DTVIERDYNALLQMLKN"
     gene            5355..5489
                     /gene="rpmH"
                     /locus_tag="PKN55_07640"
     CDS             5355..5489
                     /gene="rpmH"
                     /locus_tag="PKN55_07640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HNX71153.1"
                     /translation="MKRTYQPSVVRRKRTHGFLVRMKTRGGRAVIRARRAKGRARLGL
                     "
     gene            5495..5875
                     /gene="rnpA"
                     /locus_tag="PKN55_07645"
     CDS             5495..5875
                     /gene="rnpA"
                     /locus_tag="PKN55_07645"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012698678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HNX71154.1"
                     /translation="MAYRFCRAQRLLKTDDFSSVFRLRNSRSQRWFQVYARPNGGLPA
                     RLGLVVGKRTAKRAVARNYIKRTIREVFRLQQPALDGVDFVVRARERFDRAQGHEARK
                     ALAGLFAKLARCRASSSPSSASTS"
     gene            5833..6042
                     /gene="yidD"
                     /locus_tag="PKN55_07650"
     CDS             5833..6042
                     /gene="yidD"
                     /locus_tag="PKN55_07650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012698677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HNX71155.1"
                     /translation="MSRILIALVRFYQLAISSWRPPSCRFHPSCSAYAVEAIQRHGAF
                     KGGWLALRRIGRCHPWGKSGYDPVP"
     gene            6068..7723
                     /gene="yidC"
                     /locus_tag="PKN55_07655"
     CDS             6068..7723
                     /gene="yidC"
                     /locus_tag="PKN55_07655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014088858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HNX71156.1"
                     /translation="MDSKRLILFIALSFGILLLWQEFFAPPPKPAAVAAANAPTTAAA
                     PAPGAAPAVADAGALAAGQTVHVKTDLFDAAIDTTGGDLRGLTLSAYAAAEDESKPLK
                     LLQNGEGRTYVAQTGLISAGNPALPTHRSVFAAEKTDYALADGQNSVEVRLTAPAAQG
                     VQVSKVYTFTRGSYLIRVRYEIRNGGAIPLAASAYYRLLRDGKAPEGESRMAYTFTGP
                     AVYTEADKFQKVKFEDIDKNKANYAHAADNGWVAMLQHYFVSAWILKANDGKSVCVTA
                     QNCRFELKTAPGGLYSAGTVVDLPAIAAGATHVIDMPLYAGPQITHVLKDTAPGLELT
                     KDYGWVTIIATPLYWLLEKLHAMVNNWGWAIVLLTILVKAVFFPLSAASYRSMAKMKA
                     LAPRMERLKEQYGDDRMKFQQAMMEMYKTEKVNPLGGCLPIVVQIPVFIGLYWALLAS
                     VELRQAPWLGWIHDLTRPDPFYVLPALMAITMVGQTFLNPPPADPVQAKMMKIMPIAF
                     SVMFFFFPAGLVLYWLVNNILSIAQQWYINRSIEQAEAAKKLG"
BASE COUNT         1394 a         2466 c         2607 g         1763 t
ORIGIN      
        1 aagtgccggc gcggctccac ctggtcgccc atcagcgtgg tgaacacgtc atccgcgctc
       61 atggcgtctt cgatgcgcac tttcagcagg cggcgcacgg tggggtccat ggtggtttcc
      121 cacagttgtt ccgggttcat ttcgcccagg cctttgtagc gctggatgct gatgttgcgg
      181 cggacttcgt tcaagagcca gttcagcgct tcgccaaatt cgttgatctg gcggccttgt
      241 tcgccgcgtt tgatggcggc gccgctgtgc agcaggcctt tgagcagggc gccggtggct
      301 ttgatctggg tgtagtcgcc ggattgcagg aagctttggt cgaggtagct caccatcaca
      361 ttgccgtgca gtttgcggat gatcttcagc agatagccgc cgtttttctc gtcggcggtc
      421 agcgacaggt caatgctgtc ggccggcagc agggcgcgca gggcggccag ggcggcttcg
      481 gcgctggcgg tttcggccag gtccaccggg tcggttttca gcagggcttg cagcacgctg
      541 gggtcgatca gccggctttc gcgttcgatc accgcttgcg ccagcaggta ttggcgggcg
      601 atttcgccca gggtgtcgcc ggtcagcggg gtcatgccgt cgccggggat cagctgggcg
      661 ttgctcagcg ccagtttcag caggtattgg ttaagctcgt gctcgtcttt caggtattgc
      721 tcggttttgc cgtgcttgac cttgtacagc ggcggctggg cgatgtagat gtggccgcgc
      781 tcgaccagct cgggcatttg ccggtagaag aaggtcagca gcagggtgcg gatgtgggcg
      841 ccgtccacgt cggcatcggt catgatgatg atgcggtggt agcgcagttt ttccgggttg
      901 tagtcgtctt tgccgatgct ggtgcccagc gcggtgatca gggtggcgat ttcctggctg
      961 gcgatcagtt tgtcaaagcg ggcgcgttcg acgttgagga ttttgccctt cagcggcagg
     1021 atggcctgaa atttacggtc gcggccttgc ttggccgagc cgccggcaga gtcgccctcc
     1081 accaggtaga gttcggacat ggccgggtct ttttcctggc agtcggccag tttgccgggc
     1141 aggcccaggc cgtccatgat gcctttgcgg cgggtgattt cgcgggcttt gcgcgcggct
     1201 tcgcgggcgc gggcggcgtc cacgatcttg gagcagatgc tcttggcgtc ggcggggttt
     1261 tccagcagga aggttttcag cgattcgctg atgacttcgt tgaccaccgg gccgatttcg
     1321 ctggacacca gtttgtcctt ggtctggctg ctgaacttgg ggtcgggcag tttgaccgac
     1381 agcacgcagg ccaggccttc gcgcatatcg tcgccggtgg tgtccacctt ggcttttttg
     1441 gcgatttcgc tttgttcgat atagccgttc agcgtgcggg tcatcacctg gcgcagggcg
     1501 gtgaggtgac tgccgccgtc acgctgcggg atgttgttgg tgaagcattg cacggcttct
     1561 tgatacgagt cgttccattg catggccact tcgacggtca tgccgtcttt ttcgccaatg
     1621 ccgtagaaca ccttggggtg cagcgggttt ttgttgcggt tcatgtactg cacgaagccg
     1681 gccacgccgc ccgagaaggc aaagttttct tctttgccgt tgcgctcgtc gatcagggtg
     1741 atggccacgc cgtcattcaa gaaggacagt tcgcgaatgc gcttggccag gatgtcaaag
     1801 tggaattcca ccttgccaaa agtggcttcg ctggcgtgaa aagtcacctt ggtgccgcga
     1861 tggctggtct ggccggtttt caccagcggg gccaccggct cgccgtggtt aaactccatt
     1921 tcgtgcactt cgccattgcg ccagattttc agcttcagcc agtcggacag ggcgttgacc
     1981 accgacacgc ccacgccgtg caggccgccg gagattttgt agctgttgct gtcgaattta
     2041 ccgccggcat gcagcacggt catgatcact tcagcggcgg agcggccttc ttccgggtgg
     2101 atgtcggtgg gaatgccacg gccgttgtct tccaccgaga tggattcgtc ggggtggatg
     2161 atgactttga tggtgtcggc gtggccggcc agggcttcgt cgatggcgtt gtccagcact
     2221 tcaaacacca tgtggtgcaa gccggtgcca tcctgggtgt cgccgatgta catgccggga
     2281 cgtttgcgca cggcgtccag ccctttgagg actttgatac tgctggagtc gtattcctgc
     2341 tcgctcatga tgtggttctc tgctgtgtgt gctgcgacag cccccgccgg gtgtggcgga
     2401 ggcgttcaag ccggagcggc tccggcgggc cggggtggct cagatgcgca tcggcatcac
     2461 gatgtacttg aaatccgcct gctcgggcag ggtgaacagc gcgctgcggt tggcgtcgcc
     2521 aaacgccagt tgcagttgct ccacttgcag attggtcagc acatcggtca ggtagttgat
     2581 gttaaagccg atttccagcg gcgcgccctg gtaggcgatt tccagctctt cttcggcttc
     2641 ttcctgttcg ctgttggtgc actggatcga caggctgccc gccgccaggg tcaggcgcac
     2701 gccacggaat ttctcattgg ccagaatcgc cgcgcgttgc agcgcctgca acagcggctg
     2761 acggttgatc aaaaagatct tgtcgttatc cagcggaatc acccggttgt agtcggggaa
     2821 tttgccatcc accaccttgc tgacaatcac cgactggcca aaggcaaagc gcgcctggtt
     2881 gggcaatagc tcgatgctga cggtgtcgtc gctgtcggcc agcagcttgg ccagctcgac
     2941 aatggtcttg cgcggcaaaa tggcctcggc gcgcgccact tcggcgtcca gcgtcatgca
     3001 ggcaaaggcc aggcggtggc cgtcggtggc caccagcttg agctgattgc cttcggcgat
     3061 aaacagcatg ccgttgaggt aataacgaat gtcctgcacg gccatggcgt attgcacctt
     3121 ggccagcagc gctttcagcg ccttttgcgg cacggtcagc ttggacagcg gctcggcgcc
     3181 caccgacagc agcggaaaat cctcggccgg cagggtttgc agattaaagc ggctcttgcc
     3241 ggctttcagg gtgagcttgg cgtcttgttg gtccagcgtc accgtggcgc cgtcgggcag
     3301 cgcgcgcagg atgtcctgga gttttttggc ggaggtggtc agccgaaaat cttcgccggc
     3361 cacttcgccc tggctggcgg tggtgatctg gatttccagg tcggtggcca aaaaggacag
     3421 cgcattgccg cgcttttcaa tcagcacgtt ggccagaatc ggcagggtgt ggcggcgctc
     3481 gacaatgccg gtcaccgcat tcagcggctt gagcagcgca tcgcgttcag cttgcaggat
     3541 gtgcatggcg ggatctcccg gaatcgtgag gggcggcgcc ccagggttca tgtttttgta
     3601 tcagtcagtg tgcggctagt ttttcagcat ttgcagcagg gcattgtaat cgcgttcaat
     3661 caccgtatcg gtggtgcgca tttcggcaat ggttttgcag gcgtgcagca cggtggtgtg
     3721 gtcgcggccg ccaaaggcgt cgccaatatt gggcagcgac agctgggtga gctctttggc
     3781 cagcgccatg gcgatctggc gcggccgggc gatgtcgcgg ctgcgctttt tgctgtgcat
     3841 atcggccagc ttgattttgt aatactcggc cacggttttc tggatggttt cgatggaaat
     3901 cacccggttg ccggcggcca ggatgtcttt cagcgcttct ttcaccagct caagctcgat
     3961 agcctggttg gtgaagcgcg aatacgccac cacgcgcttg agcgcgcctt ccagctcgcg
     4021 cacattcgag cgcacgttct gggcgatgaa aaacgccaca ttgttgtcca gcttgaggtt
     4081 gtccgcctcg gcttttttca tcaggatggc cacgcgcatt tccagctcgg gcggctggat
     4141 ttccaccgtc agcccccagg aaaagcgcga aatcagccgc tcgtccatgc cttcgatctg
     4201 cttggggaag gtgtcgcagg tcatgatcac ctgcttgccg ccctcgatca gcgcattaaa
     4261 ggcatagaag aattcttcca tggtgcggtt cttgcccgca aaaaactgga tatcgtcgat
     4321 cagcagcaaa tccaggctgt ggtaatagcg cttgaattcg tcaaacgcct tgtgctgata
     4381 ggcgcgcatg atgtcggcca catagcgctc ggcgtggatg tagcggatgc gcgccttggg
     4441 gtttttctgg aacacatggt tgccaatcgc ctgcaccaag tgcgttttac ccaaacccac
     4501 gccgccatag acaaacagcg ggttatacgc cgggtcgccg gggttgtcgg caatctgcat
     4561 ggcggcggcg cgcgccagct ggttgccctt accggtcacc aggctttcaa aggtgaacga
     4621 cggattcagc cgggtgctct catggccgcc gccaatcgcc tgcacggccg ccgggcgcgc
     4681 cgacggcgag gtgggcgaag acggcggctg ggcgccagag gaggcgctgg ggctggcgga
     4741 agaagacgtc atcactggcg gctcgacctt caccggcgcg ccgattttga gctccagcag
     4801 ggccggctcg cccagcacct cgctggccag cgcctcgatg cgcgccagaa agcgatcctt
     4861 gatgaattgc agcacaaagc ggttgggcgc caagagcacc cagccctccg cccccggttc
     4921 gcagcgcagc ggcttgatcc aggtggtgaa ctgttgtgcc gacagttccg cctccagtcg
     4981 ttcgaggcag gtcggccaga atgtgtgcag atcggtcatt gattcatcta agaatgacaa
     5041 acccgccggg cacgatggcc aacggcggga gagcaggcgg ggcgggcgcg cccgcaatcc
     5101 ggcgcggcct gttgtcaggt gcgccacatc gggttctggc gcgcctggcg cgccggccgg
     5161 atgtctgccg ccgtcaggcc aaaccgggcc acaacaagaa gcagaacaaa atcagcagta
     5221 tccccattct acctgtttgc cgcgcagtta tccacagccc acacagggcc aattgcaaca
     5281 ttcagttgac aaagctgatc caagacggtc taatctcgcg tttattctcc tgatttttca
     5341 tagggatcaa gctcatgaag cgcacctacc agccttccgt cgtgcgtcgc aagcgcactc
     5401 acggtttcct cgttcgcatg aaaacccgtg gcggccgcgc cgtgattcgc gcccgtcgcg
     5461 ccaagggtcg cgcccgcctc ggcctgtaat cgccttggcc tatcgcttct gccgggcgca
     5521 gcgactgttg aaaacggatg atttttcatc cgtttttcgt ttgcgcaaca gccgcagcca
     5581 acgctggttt caggtgtacg cccggcccaa tggcggcctg ccggcccggc ttgggctggt
     5641 ggtgggcaaa cgcaccgcca agcgcgccgt ggcgcgcaac tatatcaagc gcaccatccg
     5701 cgaagtgttc cgcctgcaac agccggcgct ggacggcgtg gattttgtcg tgcgcgcgcg
     5761 cgaacgcttt gatcgcgcgc aaggtcatga agcacgcaag gcattggccg gattatttgc
     5821 taaactggcg cgatgtcgcg catcctcatc gccctcgtcc gcttctacca gttagccatc
     5881 agctcatggc gaccgcccag ctgccggttt catccgagct gctcggccta cgccgtggaa
     5941 gccatccagc gccatggcgc gttcaagggc ggctggctgg cgctgcgccg cattggccgc
     6001 tgccacccct ggggtaaaag cggctacgac ccggttccct aacccctcgc aacggttgcc
     6061 tttacagatg gattccaaac gtttaatcct gttcattgcg ctgtcgttcg gcattttgct
     6121 gctgtggcaa gaattctttg ccccgccgcc caagcctgct gccgtcgctg ccgccaatgc
     6181 cccgacaacc gccgctgcac cggccccagg cgctgcgcct gcggtggctg acgctggcgc
     6241 actggctgcc ggccagaccg tgcacgtcaa gaccgacctc ttcgacgccg ccatcgacac
     6301 caccggcggc gacctgcgcg gcctgacgct gagcgcctac gccgccgccg aagacgagag
     6361 caagccgctc aagctcttgc aaaacggcga aggccgcacc tatgtggcgc aaaccggcct
     6421 gatcagcgcc ggcaacccgg ccctgcccac ccaccgcagc gtatttgccg ccgaaaaaac
     6481 cgactacgcc ctggccgacg gccagaacag cgtggaagtg cgcctgaccg ccccggcggc
     6541 acaaggcgtg caggtgagca aggtgtacac cttcacccgt ggcagctatc tgattcgcgt
     6601 gcgctacgaa atccgcaatg gcggcgccat tccgctggcg gcctcggcct actatcgcct
     6661 gctgcgcgac ggcaaggcgc cggaaggcga aagccgcatg gcctacacct ttaccggccc
     6721 ggcggtgtac accgaagccg acaagttcca gaaggtgaaa ttcgaagaca tcgacaagaa
     6781 caaggccaac tacgcccacg ccgccgacaa cggctgggtg gccatgttgc agcattactt
     6841 cgtgtcggcc tggattctga aagccaatga cggcaagagc gtgtgtgtca cggcgcaaaa
     6901 ctgccgcttt gagctgaaaa ccgcaccggg cggcctgtac tccgccggca cggtggtgga
     6961 tctgccggcc atcgccgcag gcgccaccca tgtgatcgac atgccgctgt acgccggccc
     7021 gcagatcacc catgtgctga aagacaccgc accgggcctg gagctgacca aggattacgg
     7081 ctgggtgacc atcatcgcca ccccgctgta ctggctgctg gaaaaactgc acgccatggt
     7141 caataactgg ggctgggcga ttgtgctgct gaccatcctg gtgaaagccg tgttcttccc
     7201 gctgtcggcc gccagctacc gctcgatggc caagatgaaa gccctggccc cgcgcatgga
     7261 gcgtctgaag gagcaatacg gcgacgaccg catgaagttc cagcaggcca tgatggagat
     7321 gtacaaaacc gagaaggtca acccgctggg cggctgcctg cccatcgtgg tgcagattcc
     7381 ggtgttcatc ggcctgtact gggcgctgct ggcgtcggtg gagctgcgtc aggcgccgtg
     7441 gctgggctgg attcatgacc tgacccgccc ggatccgttc tatgtgctgc cggccttgat
     7501 ggccatcacc atggtgggcc agaccttcct gaacccgccg ccggctgatc cggtgcaggc
     7561 caagatgatg aagatcatgc cgattgcctt ctcggtgatg ttcttcttct tcccggctgg
     7621 tttggtgttg tactggctgg tgaacaatat tctgtcgatt gctcagcagt ggtatatcaa
     7681 ccgcagtatt gagcaggcgg aagccgccaa gaagcttggt taagcgttat tgcgtggtgt
     7741 gagtctctgg caagcccgtt tctttggaaa cgggcttgtt gcatgggggg tgggggtttc
     7801 tatgcctacg atgttagttt gctggggttg aattagggcg gggtttcacc ccgcacccga
     7861 ccctatttct ttgtctcgcc aaagaaatag gggaaagaaa ggcgacccgg gtggcacgcc
     7921 ctacgcctgc ggcttcgggt gccctgcgct tctcgaaaac cggggccgtc ccgaacttgc
     7981 gccggctgcg ccgtcgcttc ggacatgcgg gccggaaagc cccccggttt tctgcgatgc
     8041 tcggcgcgcc acacgggggt aaacggcggc taagcacaac gggcggtaag atggcctata
     8101 tggtaaaggc gcacgacaca gtgctatccc atggtgctat cccatatagg ccgcaccacc
     8161 ccggcactac cctcacccca cgccacttca cccgttttca ggaatttgtc atgtccgctg
     8221 ttcgtttgcc
//
LOCUS       DAOPYX010000124         8211 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_10425_length_8211_cov_9.967631, whole genome shotgun sequence.
ACCESSION   DAOPYX010000124 DAOPYX010000000
VERSION     DAOPYX010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 8211)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8211)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8211
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10425_length_8211_cov_9.967631"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1006
                     /locus_tag="PKN55_07660"
     CDS             <1..1006
                     /locus_tag="PKN55_07660"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012696039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HNX71157.1"
                     /translation="RLFQKYPRLARDLARQLGKEVELVIIGEETELDKTMIEDLNDPL
                     VHLVRNAVDHGVETPEERVAVGKKPQSVIELSAQQVGDHILIEITDDGKGMRAEMLRR
                     KAVEKGLIDLETANSLDDKQALQLIFLPGFSTKDQISNVSGRGVGMDVVRTNIQKLNG
                     RIDINSEVGEGTRISISLPLTLAILPVLVVKVCNQPFAVPLAMVREIIPIQQDSIQEV
                     SGRPTIVVRDEILPVRSLADLLGWQPTQEAQFGVLMQSAERAFILAIDAFVGRDDVVI
                     KPLQNIRPRGVAGATLSGDGSVVLVLDMEDLLSDPSANQQNVTPALGNNAAMPRLTAA
                     "
     gene            1181..2056
                     /locus_tag="PKN55_07665"
     CDS             1181..2056
                     /locus_tag="PKN55_07665"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0019674 - NAD metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HNX71158.1"
                     /translation="MNSMFKRVALVARHSKPQVVASLISLADFLTTHGLTVLVDRDSV
                     SHADAGRYPLVDRADLGKLADLAIVLGGDGTMLSVARLLAPYNTPMVGVNQGRLGFMT
                     DISLHEMFTTIEAILHGEFVPEDRILLNASVQRDGVEVASTLAFNDVVFSRGATGSMI
                     EFEIFIDKQFVYSQRSDGLIVSTPTGSTAYSLAAGGPILHPTIPAITLVPICPQSLSN
                     RPIAVNDSCLVEFVLTRGVDSRVHFDGQSHCDLQEMDRVLIRRYRNPLRILHPVGYNY
                     YDMLRHKLHWGERLL"
     gene            2086..3747
                     /gene="recN"
                     /locus_tag="PKN55_07670"
     CDS             2086..3747
                     /gene="recN"
                     /locus_tag="PKN55_07670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014086950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HNX71159.1"
                     /translation="MLLSLAIRDFVIVDTLELDFTAGFTVLTGETGAGKSIIIDALSL
                     LLGGRGDGSLVRAGCERAELSARFDLARLPVLADWLAEQALADEGGELLVRRMLDASG
                     RSRCFLNGRAATLAQLKTAGEWLVDIHGQHAHQSLVKPDTQRELLDAYAQSGELVASV
                     AERHRIWQDARQARLDAEQHAAAYALERERLTWQINEVNELNPQPGEWDTLGQQHQRL
                     ANAAELQDEARHALNALSDDEGNLISAVAQLQGRLTRFAGVDTRLAPVAALLDSVDAE
                     LREALYALRDYVEDIEQNPEELAQVEKRIDALHACARKFRLRPEDLPQRLADWRQQLA
                     ELDNVADLDGLLASEAEALSALREAAEVLSDRRRRAASELAERISAEMHTLAMAGSRF
                     DIVLTPLATPSAHGAESVEYQVAANAGSPLRPLAKVASGGELSRISLALQVVTSAVAA
                     VPTLIFDEVDVGIGGRVAEVVGKLLQRLGEQYQVLCITHLPQVAARGDVQWQVSKSGE
                     GEAGAVISRIRPLSSKERVEEIARMLGGEQITATTRRHAREMLAG"
     gene            complement(3844..4323)
                     /gene="rraA"
                     /locus_tag="PKN55_07675"
     CDS             complement(3844..4323)
                     /gene="rraA"
                     /locus_tag="PKN55_07675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008954283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008428 - ribonuclease inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0051252 - regulation of RNA metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HNX71160.1"
                     /translation="MSFQTTDLCDAHSDHLRVVAPMFKQFGGQVKFSGQVVTLKLFED
                     NSLVRDTLAKPGAGRVLVVDGGGSLRCALVGDQIAELAVKNQWAGIVVYGCIRDSAAI
                     NGLPIGVRALDTHPLKSIKKGVGDVDIPVTFGGVTFTPGDWLYADEDGVIVSAAPLL"
     gene            complement(4414..5904)
                     /locus_tag="PKN55_07680"
     CDS             complement(4414..5904)
                     /locus_tag="PKN55_07680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007341776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HNX71161.1"
                     /translation="MTLTRIRPLVLSLALIGALSGCDRLQQLRDYFSGSSSSQAPVAI
                     PGQGGQVNMLLPDFTQLVEREGPAVVNISANRMEDEEDSPFPFPIPEDDPFFEFFKRF
                     VPPNGAPRQQQPRERESVSYGSGFILSEDGYLLTNAHVVAGASQVQVKLNDKREFKAK
                     VIGVDKRTDVAVLKIDASGLPKVKIGAPATLKVGEWVAAIGAPFGFDNTVTAGIVSAK
                     GRSLPDENYVPFIQTDVAINPGNSGGPLFNLRGEVVGINSQIYSRSGGFMGISFAIPI
                     DIAVGVAEQLKTKGKVSRGQLGVHIQELSQELARSFGRPNAEGALIVNIEPNGAAAKA
                     GVQVGDIVLAFDGKKVESSKDLPMLVGAKAPGSKAKLTLWRKGEEKTLDVVLGELASS
                     ERAANEKPQTQAAPKMSFDKIGLAVSELSREQRADSGLSSGLLVEKASGLAAKAGLSA
                     GDVILAVNHSPVSELKTFAGLLQGDAVALLVRRGDSTLFLPLRLRE"
     gene            complement(5918..6583)
                     /gene="nth"
                     /locus_tag="PKN55_07685"
     CDS             complement(5918..6583)
                     /gene="nth"
                     /locus_tag="PKN55_07685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008954285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HNX71162.1"
                     /translation="MNADKRAAIYARLAQAIPAPTTELEYQSPFELLTAVLLSAQATD
                     VGVNKATRKLFPVANTPAAMLALGIEGLEPYIKTIGLYRSKARHLIETCRLLLERHGG
                     EVPAERAALEALPGVGRKTANVILNTAFGHPTIAVDTHIFRVANRTRLAPGETVREVE
                     DRLVKWTPKAYRMDAHHLLILHGRYVCKARKPLCEACVIADLCEYPARTSQTPAPLPD
                     AAD"
     gene            complement(6580..7278)
                     /locus_tag="PKN55_07690"
     CDS             complement(6580..7278)
                     /locus_tag="PKN55_07690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018749241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit E"
                     /protein_id="HNX71163.1"
                     /translation="MSQWISGDAARGIIGDGLWRQNAGLVQLLGLCPVLAVSSSVVNG
                     VSLGLATALVTGLSSALIASVRHWVPNEIRNPVFILLIAALVTLVELLMRAGLPELYA
                     VLGIFIPLITTNCIVLARAEAFASRHPVPHAALDGVMMGLGLTLVLAVLGGLRELFGK
                     GTLLSGLELVLGESARGWVIHLVPESAHYQFLLAMLPPGAFIGLALLIAGKQWWEARP
                     ARTPAPAPLQGEQA"
     gene            complement(7275..7913)
                     /gene="rsxG"
                     /locus_tag="PKN55_07695"
     CDS             complement(7275..7913)
                     /gene="rsxG"
                     /locus_tag="PKN55_07695"
                     /inference="COORDINATES: protein motif:HMM:NF002519.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HNX71164.1"
                     /translation="MSALHDAVQRARASGLRLALFALVCAALLAAVEALTRDTLRDYA
                     EASRRATLAQVLPAGSFDNDVLADSVALSEADAALLGLRPPAVAHRARRGGQFSGWVV
                     ESVAANGYAGAIRMLVGVDANGVISGVRVIEHRETPGLGDYIDAARSPWSQQFIGRRI
                     SDGLRWQVKKDGGPFDAMAGATISARAVTAAVGRAANVLARLPAEHNEGKQP"
     gene            complement(7910..>8211)
                     /locus_tag="PKN55_07700"
     CDS             complement(7910..>8211)
                     /locus_tag="PKN55_07700"
                     /inference="COORDINATES: protein motif:HMM:NF015100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HNX71165.1"
                     /translation="LAHLLDAERYASPLFHLLAGATQLGAWFIATDPVTAPSSARGRL
                     WFGAGIGALTWVIRTWGGYPDAVAFAVLLMNLCAPFIDLHTRAPAFGQRQPGGRR"
BASE COUNT         1452 a         2713 c         2624 g         1422 t
ORIGIN      
        1 ccgcctgttc cagaagtatc cacgcctggc gcgcgatctg gcccgccaac tgggcaagga
       61 agtcgaactg gtcatcattg gcgaggaaac cgaactcgac aagaccatga tcgaggatct
      121 gaacgatccg ctggttcacc tggtgcgcaa tgctgtcgat cacggtgtgg aaaccccgga
      181 agagcgcgtg gcggtgggca aaaagccaca aagcgtgatc gagctgtctg cccagcaggt
      241 aggcgaccat atcctgatcg agattaccga cgacggcaag ggcatgcgtg ccgaaatgct
      301 gcgtcgcaag gccgttgaaa aaggcctgat cgacctggaa accgccaaca gtctggacga
      361 caagcaagcg ctgcaactga tcttcctgcc cggcttctcc accaaggacc aaatctccaa
      421 tgtgtccggc cgtggcgtgg gcatggatgt ggtgcgcacc aatatccaga agctgaatgg
      481 ccgcatcgac atcaactcgg aagttggcga aggcacccgc atcagcatct cgctgccgct
      541 gacgctggcc attctgccag tgctggtggt caaggtgtgc aaccagccgt ttgccgtgcc
      601 gctggccatg gtgcgcgaga tcatcccgat tcagcaggac agcatccagg aagtctccgg
      661 ccgtccgacc atcgtggtcc gcgacgaaat cctgccggtg cgctcgctgg ctgatctcct
      721 gggctggcag cccacccagg aagcccagtt tggcgtgctg atgcagtcgg ccgaacgcgc
      781 cttcatcctg gccatcgacg cctttgtcgg ccgcgacgac gtggtgatca agccgctgca
      841 gaatatccgt ccgcgtggcg tggcgggtgc cacgctgtct ggcgatggtt cggtggtgct
      901 ggtgctggat atggaagacc tgctgtctga tccgtcggcc aaccagcaga acgtaacacc
      961 tgcgctgggc aacaacgccg ccatgccgcg cctgaccgcc gcatgatccg cgtgagttta
     1021 tctgcctgag cacaaagccc ccattcgggg gctttgttgc atctgaatgt caggcacgcg
     1081 ccttgcatgc tcacagtatg ccgcagaaat ccacccgaat ttgacctgac gccgtgcaag
     1141 ccgcttggcg tgactgtccg caaatgattt aattcgcccc atgaattcca tgttcaagcg
     1201 tgtggcgctg gtcgcccgac acagcaagcc ccaggtggtc gcttcactga tctcgctggc
     1261 cgattttctg actacgcatg gtctgaccgt gctggtggac cgtgacagcg tgtcgcatgc
     1321 cgatgccggt cgctacccat tggttgatcg cgcagacctg ggcaaactgg ccgatctggc
     1381 cattgtgctg ggcggcgatg gcaccatgct gtcggtggcg cgtctgctgg cgccatacaa
     1441 cacgcccatg gtgggggtca accaaggccg gctgggcttt atgaccgaca tctcactgca
     1501 tgagatgttc accaccatcg aggccatcct gcatggtgaa ttcgtgccgg aagaccgcat
     1561 tctgctcaat gccagcgtgc agcgcgatgg tgtggaagtg gccagcaccc tggcgtttaa
     1621 tgacgtagtg ttcagccgtg gtgccaccgg ctcgatgatc gaattcgaga tttttatcga
     1681 caagcagttt gtctacagcc agcgctcgga cggcctgatt gtctccaccc ccaccggctc
     1741 cactgcctat tcactggccg cgggcggccc catcctgcat cccaccatcc cggccatcac
     1801 cctggtgccg atctgcccgc aatcgctgtc caaccggccg attgccgtca acgacagctg
     1861 cctggtcgaa ttcgtgctga cccgtggagt ggatagccgg gtgcattttg acggccaatc
     1921 gcactgcgac ctgcaagaaa tggatcgcgt gctgattcgc cgctatcgca acccgctgcg
     1981 catcctgcac ccggttggct acaactacta cgatatgctg cgccacaaac tgcattgggg
     2041 cgaacgcctg ctctgatttt gcgaccgcaa cccggatctc acgccatgct gctctcgctc
     2101 gccatccgcg actttgtcat tgttgacacc ctggaactgg atttcaccgc cggctttacc
     2161 gtgctcactg gcgaaaccgg cgccggcaaa tccatcatca tcgacgccct gtcgctgctc
     2221 ttgggcggcc gcggcgacgg cagcctggtg cgcgccggct gcgagcgcgc cgagctctcg
     2281 gcccgctttg acctggcccg actgccggtg ctggccgact ggcttgccga acaagcgctg
     2341 gccgacgaag gcggcgaatt gctggtgcgg cgcatgctgg acgccagcgg ccgctcgcgc
     2401 tgttttctga atggccgcgc cgccaccctg gcgcagctga aaaccgctgg cgaatggctg
     2461 gtggacatcc acggccagca cgcccaccaa tcgttggtca aacccgatac ccagcgcgaa
     2521 ctcctggatg cctacgccca gtcgggtgag ttggtcgcca gcgtggccga acgccaccgc
     2581 atctggcagg acgcccgcca ggcgcggctg gacgccgagc aacacgccgc cgcctatgcg
     2641 ctggagcgcg agcggctgac ctggcaaatc aacgaagtca acgaactgaa cccgcagccg
     2701 ggcgaatggg acaccctggg ccagcagcac cagcgactgg ccaacgccgc cgaattgcaa
     2761 gacgaagcac gccatgcgct gaacgcgcta tctgacgacg agggcaatct gatttccgcc
     2821 gtggcgcagc tgcaaggccg tttgacccgc tttgccggcg tggatacccg gctggcaccg
     2881 gtggcggcgc tgctggactc ggtggacgcc gaattgcgcg aggcgctgta cgccctgcgc
     2941 gactatgtgg aagacatcga acaaaacccg gaagaactcg cccaagtcga aaaacgcatc
     3001 gacgccctgc acgcctgcgc gcgcaaattc cgcctgcgcc cggaagacct gccgcagcgc
     3061 ctggccgact ggcgtcagca attggctgag ctggacaatg tcgccgacct ggatggcctt
     3121 ttggccagcg aggccgaagc gctgagcgcg ctgcgtgaag ctgctgaagt gctgtccgac
     3181 cggcgacgcc gcgccgccag tgaactggcc gagcgcatca gtgccgaaat gcacaccctg
     3241 gccatggccg gcagtcgttt tgacatcgtg ctgaccccgc tggccacccc cagcgcgcac
     3301 ggcgccgaaa gcgtggaata ccaggtcgcc gccaatgccg gctcgccgct gcgcccgctg
     3361 gccaaggtgg cttctggcgg cgagctgtcg cgcatcagcc tggccttgca agtggtcacc
     3421 agcgcggtgg cggcagtgcc gacgctgatt tttgacgaag tggacgtcgg catcggcggc
     3481 cgggtggccg aagtggtcgg caaactgctc cagcgtctgg gtgagcaata tcaggtgctg
     3541 tgcattaccc acctgccgca agtggcggcg cgtggcgatg tgcaatggca ggtgtccaaa
     3601 tccggcgagg gtgaggccgg cgcggtgatc agccgcatcc ggcccttgtc cagcaaggag
     3661 cgggtggaag agattgcccg catgctgggc ggcgagcaga ttaccgccac cacccgtcgt
     3721 cacgcgcggg agatgttggc ggggtagtgg ggcgggattg agggcaggat ggcatcccgt
     3781 cctcgtcccg ccagtgttgt cttgccaaag aatccggtgg aagaacaacc caccgaatgc
     3841 tgtttacagc agcggggcgg cgctgacgat cacgccgtct tcatcggcgt acagccagtc
     3901 gccgggggtg aaggtcacgc cgccgaaagt caccgggatg tccacatcgc cgacgccctt
     3961 tttgatggat ttgagcgggt gggtgtccag cgcgcgcacg ccaatcggca ggccgttgat
     4021 ggcggcagag tcgcggatgc agccatacac cacaatgccg gcccattgat ttttcaccgc
     4081 cagttcggca atctggtcgc ccaccagcgc gcagcgcagc gagccgccgc cgtccaccac
     4141 cagcacgcgg ccggcgcccg gcttggccag ggtgtcgcgc accagcgaat tgtcttcaaa
     4201 cagtttcagc gtgaccacct ggccgctgaa cttcacctgg ccgccaaact gcttgaacat
     4261 cggcgccacc acgcgcagat ggtcgctgtg ggcgtcgcac aggtcggtgg tttgaaagct
     4321 catgattcat cctttgtcgt ctgagaggga ggggaagcgc cacccgctgg cgcgccggtc
     4381 tgcgccggct gacgacaggc gggcagcggc gggttattcg cgcaggcgca gcggcaggaa
     4441 cagcgtgctg tcgccacggc gcaccaacag cgccacggcg tcgccctgca acaggccggc
     4501 aaaggttttc agctcgctca ccgggctgtg gttgaccgcc agaatcacat cgccggcgct
     4561 cagcccggcc ttggcggcca ggccgctggc tttttccacc agcaggccgc tgctcaggcc
     4621 ggagtcggcg cgttgttcgc ggctgagttc gctgacggcc agaccgattt tgtcaaagct
     4681 catttttggc gcggcttgtg tctggggttt ttcgttggcg gcacgttcgc tgctggccag
     4741 ctcgcccagc accacgtcca gggttttttc ttcgcccttg cgccacagcg tcagcttggc
     4801 cttgctgccc ggcgccttgg cgcccaccag catcggcagg tctttcgagc tttccacttt
     4861 cttgccatca aacgccagca caatatcgcc cacctgcacg ccggccttgg ccgccgcgcc
     4921 attgggttcg atattgacga tcagcgcgcc ctcggcgttg gggcggccaa acgagcgggc
     4981 cagttcttgc gacagctcct gaatatgcac gcccagctgg ccacggctga ccttgccctt
     5041 ggttttcagc tgctcggcca cgcccacggc gatgtcgatg gggatggcga aagaaatgcc
     5101 cataaagccg ccggagcggc tgtaaatctg cgaattgatg cccaccactt cgccgcgcag
     5161 attgaacagc gggccgcccg agttgccggg gttgatggcc acgtcggtct ggataaaggg
     5221 cacatagttt tcgtcgggca ggctgcggcc cttggccgag acaatgccgg cggtgacggt
     5281 gttgtcaaag ccaaacggcg cgccaatcgc cgccacccat tcgcccactt tcagcgtggc
     5341 cggcgcgccg atcttgacct tgggcaggcc gctggcgtcg atcttgagca ccgccacatc
     5401 ggtgcgtttg tccacgccaa tcaccttggc cttgaattcg cgcttgtcgt tgagcttgac
     5461 ctgcacctgg ctggcgccgg ccaccacgtg ggcattggtc agcaaatagc cgtcttcaga
     5521 caagataaag cccgagccat acgacacgct ttcgcgttcg cgcggctgtt gctggcgcgg
     5581 tgcgccattg ggcggcacaa agcgcttgaa gaactcaaag aacgggtcgt cttccggaat
     5641 cgggaagggg aagggcgagt cttcttcgtc ttccatgcgg ttggcgctga tattgaccac
     5701 tgccgggcct tcgcgctcga ccagctgggt gaagtcgggc agcagcatat tcacctggcc
     5761 gccctggcca ggaatcgcca cgggcgcctg actggaggac gaaccagaaa aataatcacg
     5821 cagctgttgc aggcggtcgc agccggacaa ggcgccgatc agcgccagcg acaacaccag
     5881 gggacgaatg cgggtcaggg tcacgggata catcctttca gtctgcggca tcgggcagcg
     5941 gcgccggcgt ctggctggtg cgcgccggat actcgcacaa atcggcaatc acacaggctt
     6001 cgcacaaggg cttgcgcgcc ttgcacacat agcggccgtg cagaatcagc aaatggtggg
     6061 cgtccatgcg gtaggccttg ggcgtccatt tgaccaggcg gtcttccacc tcgcgcaccg
     6121 tctcgcccgg cgccagccgg gtgcggttgg ccacccgaaa aatatgggtg tccaccgcga
     6181 tggtggggtg gccaaaggcg gtgttcagaa tcacattggc ggttttgcgg cccacgccgg
     6241 gcagcgcttc cagcgcggcg cgctcggccg gcacctcgcc gccatggcgc tccagcagca
     6301 gccgacaggt ttcgatcaga tggcgcgcct tgctgcggta caggccaatg gtcttgatat
     6361 agggttccag gccttcgatt ccaagggcca gcatcgccgc cggggtattc gccaccggaa
     6421 acagcttgcg ggtggcttta ttgacgccaa cgtcagtcgc ctgcgccgac agcagcacgg
     6481 cggtgagcag ctcaaacggc gattgatatt ccagctcggt ggtgggcgcc gggatcgcct
     6541 gggccagccg ggcgtaaatg gccgcgcgtt tgtcggcgtt catgcctgtt ctccctgcag
     6601 cggggcaggc gctggcgtgc gcgctggccg cgcctcccac cattgcttgc cggcaatcaa
     6661 cagcgccagg ccgataaacg cccccggcgg cagcatggcc agcaaaaact gatagtgcgc
     6721 cgattccggc accaaatgaa tcacccagcc gcgcgccgat tcgcccagca ccagctccag
     6781 cccggacagc agcgtgccct tgccaaacag ctcgcgcagc ccgcccagca ccgccagcac
     6841 cagggtcagc cccaggccca tcatcacccc gtccagcgcc gcgtgtggca ccgggtggcg
     6901 cgaggcaaac gcctcggcgc gcgccagcac aatgcagttg gtggtgatca gcgggataaa
     6961 aatccccagc acggcgtata gctcaggcag gccggcgcgc atcagcagct ccaccagcgt
     7021 caccagcgcg gcaatcaaca aaataaacac cggattgcgg atttcattgg gcacccagtg
     7081 gcgcacgctg gcaatcagtg cgctggacaa gccggtgacc agtgcggtgg ccaggcccag
     7141 gctgacgccg ttcaccacgc tgctgctcac cgccagcacc gggcacagcc cgagcaattg
     7201 caccagcccg gcgttttgtc gccacaggcc atcgccgata atgccgcgcg cggcgtcgcc
     7261 gctgatccat tggctcatgg ttgtttgccc tcgttgtgct ctgccggcag ccgcgccagc
     7321 acattggccg cgcggccaac ggcggcggtg acggcgcgcg cgctgatggt ggcgccggcc
     7381 atggcgtcaa atgggccgcc gtcttttttg acctgccagc gcaggccgtc gctgatgcgg
     7441 cggccgatga actgctggct ccatgggctg cgcgcggcgt cgatatagtc gcccagcccc
     7501 ggggtttccc ggtgttcaat cacccgcacg ccgctgatga cgccattggc gtccaccccc
     7561 accagcatgc ggatggcgcc ggcgtagcca ttggcggcca ccgattccac cacccagccg
     7621 ctgaactggc cgccgcgccg ggcgcggtgc gcgacggcgg gcgggcgcag gcccagcagc
     7681 gcggcgtcgg cctcgctcag cgccacgctg tcggccagca catcgttatc aaagctgccg
     7741 gccggcaaca cctgcgccag cgtggcgcgg cggctggcct cggcgtagtc gcgcaaggtg
     7801 tcgcgggtga gcgcttccac cgccgccagc agggcggcgc acaccagggc aaacagcgcc
     7861 aggcgcaggc cgctggcgcg cgcgcgttga accgcatcgt gcagggcgct catcgtcggc
     7921 ctccgggctg gcgctggcca aaggccggcg cgcgggtgtg caggtcgatg aacggcgcgc
     7981 acaaattcat cagcagcacg gcaaacgcca ccgcgtccgg gtagccgccc caggtgcgta
     8041 tcacccaggt cagcgcgcca atgccggcgc caaaccacag ccggccgcgc gcgctgctgg
     8101 gcgcggtgac cgggtcggtg gcgataaacc aggcgcccag ctgggtggcg ccggccagca
     8161 gatgaaacag cgggctggcg taacgctcgg cgtccagcag gtgggccagg g
//
LOCUS       DAOPYX010000125         8178 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_10499_length_8178_cov_8.649268, whole genome shotgun sequence.
ACCESSION   DAOPYX010000125 DAOPYX010000000
VERSION     DAOPYX010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 8178)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8178)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8178
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10499_length_8178_cov_8.649268"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            117..5783
                     /locus_tag="PKN55_07705"
     CDS             117..5783
                     /locus_tag="PKN55_07705"
                     /inference="COORDINATES: protein motif:HMM:NF019323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HNX71166.1"
                     /translation="MSKLVPLLLSLWSSLALSAGVASFSPQGENPRPEQLRITFSTPM
                     TALAAGATAPAPIRWDCPLNGAGRWVDDKNWVFSLSATPQANTTCAISLMPGLKDLKG
                     DVLPAARFAFRTGAPSIVRSWPFDSSQVDEDQVFALQFNTVLASTPKVYCQSSAWPEQ
                     LLMQPIAVEARRRLLAHLANDEGLAPKDADRVATLSCPRRLPADSALLLRLARDDQQV
                     QRINYSVRAPFTATVRCQRENARAQCNPLTAISLVFSEPILRAQAAAIRLGGKAAGLG
                     QQEREVTELVFAPPFTPRQTLGLSLPANVRDVSGRLLSNLAELQSGVRIADYSPLAKF
                     SAAPFGVLEAADPVLPLTLRGVEGNLSGVTPQLRGKTLWLNDDRAMMRWLGKFEQYHE
                     SSIPPSRQLEGAQPVESRRLSLLNSQRQASELKLPSTPDKQGHWPFEVIGLPLPKRGL
                     HLVELESRVLGQALLGEPQPMYVRTAALVTNLAVHLKITPENAAVWVSTLNRARPVAK
                     ASVRVYDCTQALLWQGDTDAQGVARIPHALKEPQCDKPGELSGLFVVARARDAQGTED
                     MAFARSGWNQGLEAWRFPFSAPEDGAPPLVAHSILDRSLLRPGETVSMKHILRTRDAR
                     GLHVFEHDALPDEVRISHKQSEQTYTLPLTWRQRRYAETRFTLPVNAALGEYSITLVR
                     KGRRVSGAEQAANPRDDGYQLESSAFRVEEFVLPALFGQIHAAPGAGVAARELPLSVQ
                     VNWANGGPARDWPLTVSAMLHPRESGVAGYETYSFEPMEDTGAQALNGKLVLDKAALK
                     LDAQGNGRLLISRLPALDRPYWLKTEASLSDPSGERQTVSRWIALWPAATRVGVTASP
                     AGKDGARWRVDALTLDLAGKPQAGRKLRVRMVGHDTLSARKRVVGGFYAWEHAQTRRD
                     EGEVCQGVSDSQGRFDCQVTVKPHEQVEFIAEAEDDRGQRARASYSVWPASGEDAWFD
                     AADHDRIDIIPDKTEYQPGERARFQVRMPFRRATAWVAIERGGVLDTRVIELDRPEFE
                     LDIAPDWTPNVYVSVLAVRGRLREVPWYSFFAWGWRRPKDWWDAFWHERPDYTPASAL
                     VDLSRPAVKYGIAQIRVGDRAHRLSVTVTPERERYGVRQTARARVQVRLPDGSPAPAG
                     SEVAFAAVDEALLELQPNASWNLLREMFPDYGYRGEIATSQQQVVGKRHYGRKALPPG
                     GGGGKAPTRQLLDTLLTWQPAVKLDARGEAMVDIPVNDVLSRFRLVAVADVGEQLFGA
                     GEASFEVAQDVQLVSGLSPLTREGDAMTAAVTVRNGSKRPLTLTVSASRDSQTLPAQR
                     LTLAAGDARQLSWAVTTPAGVSVQQWLFAARAEDGSASDRLSLRQRVEPAVPVSVWQG
                     AFIRLEGEQSLPVALPAGALPGQGGVRVSVQSRLAGELPGVARWLEDYPFSCLEQRAA
                     VALGRRDAAAWAKLQEDLPGYLDEHGLADYFPRSSANGPSGSDVLTSHLLSVSHEAGQ
                     PLPDALRERMLAGLSAFVEGRIKRELPGGAAGLDARRLAAMLALQRHGRFRPAMLDVL
                     SVQPQTWPTPMLVNWAELLLRQPAIPARDARLAQASQLLRARLLYQGARLALAREQQD
                     SAWWLMEDGDLASARLLWLASQLPDWRDDAPRLAVGLLARQQGGHWRTTTANLWGVLA
                     TRAFSAQFERVPVRGQTTAALGEARATVTPAEAPVSAPLLPWPASGQGTLRLSHQGSG
                     APWASVLALAAVKFDGQRSAGYSVQKTLTPLSQRTPGQYSPGDVLKVTLDIRAEAGMG
                     WVALNDPIPAGASLLGSGLGRDSQIAVSQAGNDDSVTPSFVERRHSGYLAYFRQLPAG
                     VHRLSYTLRLNQAGVFQLPPTRIEAMYAPEQYGMLANAPLTVTHAR"
     gene            5803..7980
                     /gene="pbpC"
                     /locus_tag="PKN55_07710"
     CDS             5803..7980
                     /gene="pbpC"
                     /locus_tag="PKN55_07710"
                     /inference="COORDINATES: protein motif:HMM:TIGR02073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1C"
                     /protein_id="HNX71167.1"
                     /translation="MLVCGLMLASTAQATPSLAEVKAAWLASDVVYVDRHGEEIQRLR
                     RDKQARRQDWVSLAEMSPALRHALVLSEDRRFYQHSGVDWSGVAAAAWANLWNTRTRG
                     ASTLTMQLAGLLDAELKAGAGGRSVWQKLGQGWSAVALERHWRKAEILEAYLNLVSFR
                     GELVGVGAVSRALFDKLPDGLNRQEAAILAALLRGPNADAATLSRRACTVYQDMQADE
                     AVQLHLPAPDAAAGQRACQQLTLLTQQALGRRQGGQALTVQLAPHFARKLAEQRRDPA
                     GRVVRTTLDARLQRLAAAVLHRHLASLARRHVEDGAVLVLDNASGDILAWVGSSGELS
                     AAREVDGVTAPRQAGSTLKPFLYQLALQQRYLTAAALLDDSPLDMATAGGLYSPQNYS
                     RDFKGLVSVRSALGASLNIPAVRTVAMVSPRLLRDRLHALGLGTLTRDGDFYGYSLAL
                     GAADVRLLDLTNAYRTLAQGGLASPPRWTLDAPPARPVRLLDAASSFIIADILADRGA
                     RSRTFGLESALSTRYWSAVKTGTSKDMRDNWALGFSRRYTVGVWVGNASGQPMWDVSG
                     MHGAAPVWLDVLNALHQHTPSPPPAPPAGVQRQRVHYADEVEAEREEYFLAGSEQREI
                     VAVRASDSQAPGIVSPLPGSVYAIDPDIPPKHQRLLLRARGVPGAQWRIDGRPAGQGD
                     AVPWFPWPGRHRLALLNAQGQPVQTLTFDVRGAVAKPGARPPR"
BASE COUNT         1258 a         2687 c         2849 g         1384 t
ORIGIN      
        1 gccagggccg ctgtgctgga tttgctgagc ggcgccaggc ggcgggttga tcttcaatag
       61 gggtttttga cgcggctcag gcataatcac ccgatcgtca tcccccggcg cggatcatgt
      121 ccaagcttgt gcctttgctg ttgtctttgt ggtcatcgtt ggcgctgagc gctggcgtgg
      181 ccagtttttc tccccagggt gaaaatcccc ggcctgaaca actgcgcatc accttcagta
      241 cgccgatgac ggcgctggct gccggcgcca ctgccccggc gcccatccgc tgggattgtc
      301 cgctgaacgg cgctggccgc tgggtggacg acaaaaactg ggtgttcagt ctgtccgcca
      361 cgccgcaggc caacaccact tgcgccatca gcctgatgcc cggcctgaaa gacctcaagg
      421 gcgatgtcct gcctgcggcc cgctttgcct ttcgcaccgg tgcgccgtcc atcgtgcgca
      481 gttggccatt cgacagcagc caggtggatg aagaccaggt ctttgccctg caattcaata
      541 ccgtgctggc cagtacgccc aaggtgtatt gccaatcctc tgcctggcca gagcagctat
      601 tgatgcagcc catcgcggtc gaggcgcgcc gccgcttgct ggcccatctg gccaatgacg
      661 agggcttggc gccaaaagac gccgaccggg tggccacgct cagttgcccg cgccgtttgc
      721 ctgctgacag cgccttgctg ttgcgcctgg cgcgtgatga tcagcaggtg cagcgcatca
      781 attattcggt gcgcgcgccg ttcaccgcga cagtgcgttg ccagcgtgaa aatgcccgcg
      841 cgcaatgcaa tccgctgacc gcgatctccc tggtttttag cgagccgatt cttcgcgccc
      901 aggccgccgc catccggctg ggcggcaagg cggccggatt gggccagcag gagcgcgaag
      961 tgacggagct ggtatttgcg ccgcccttca caccccgtca gacgctggga ctcagcttgc
     1021 cggccaatgt gcgggatgtc agcggccggc tgctgagcaa tctggccgag ctgcaaagcg
     1081 gcgtgcgcat tgctgactat tcgccgttgg ccaaattcag cgcggcgccg tttggcgtgc
     1141 tggaagccgc cgacccggtg ctgccactga cgctacgcgg ggtggaaggc aatctgtctg
     1201 gcgtcacccc gcaattgcgc ggcaagacac tgtggctgaa tgacgatcgc gccatgatgc
     1261 gctggctggg caagttcgag cagtatcacg aatcctctat tccaccaagc cgccagcttg
     1321 agggcgcgca gccggtggaa agccgccggc tgagcctgct gaacagccag cgccaggcca
     1381 gcgagctgaa gttgccgagc acgccagata aacaaggcca ctggccgttt gaggtgattg
     1441 gcctgcccct gccaaaacgc ggcctgcatc tggtcgagct ggaatcgcgc gtcttgggcc
     1501 aggccttgct gggcgagccg cagccgatgt atgtgcgcac tgccgcgctg gtgaccaatc
     1561 tggctgtgca tctgaaaatc accccggaaa acgcggccgt gtgggtcagc acgctcaatc
     1621 gcgcccggcc ggtggccaag gccagtgtgc gggtgtacga ctgcacgcaa gccctgctgt
     1681 ggcaaggcga caccgacgcc cagggcgtgg cgcgtattcc ccatgccctg aaagaaccgc
     1741 agtgcgacaa gccgggcgag ctgagcggac tgtttgtggt ggcgcgcgcg cgtgatgccc
     1801 agggtactga agacatggcc tttgcccgca gcggctggaa tcaaggcctg gaagcctggc
     1861 gctttccgtt ttcagcaccg gaggatggcg caccaccctt ggtggcgcac agcattctgg
     1921 accgcagcct gctgcgcccg ggcgaaaccg tttccatgaa gcacatcttg cgcacgcgcg
     1981 atgcgcgcgg tctgcatgtg tttgagcacg atgccctgcc cgacgaagtg cgcatcagcc
     2041 acaagcagag cgagcaaacc tataccctgc cgctgacctg gcggcagcgc cgctatgcgg
     2101 aaacccgctt taccctgccg gtcaatgccg cgctgggcga atacagcatc accctggtgc
     2161 gcaagggccg acgcgtgagc ggtgcggagc aggcggccaa cccgcgcgat gatggctacc
     2221 agctggaaag ctcggctttt cgggttgagg agtttgtcct gcccgccttg tttggccaga
     2281 tccatgccgc gcccggcgct ggcgtggcgg cccgtgaatt gccgctgagc gtgcaggtga
     2341 actgggccaa tggcgggcct gcgcgcgact ggccgctgac cgtcagcgcc atgctgcacc
     2401 cccgcgagag cggagtggcc ggctacgaga catacagctt tgaaccgatg gaagatactg
     2461 gcgcgcaggc gctgaatggc aagctggtgc tggacaaggc cgcgctcaag ctggatgccc
     2521 agggcaatgg ccgcctgctg atctccaggc tgccagcgct ggatcgcccc tattggctga
     2581 aaaccgaagc ctcgctcagc gacccgtccg gggagcggca aaccgtgtcg cgctggattg
     2641 ccctgtggcc ggcggccacc cgcgtgggcg ttaccgccag cccggccggc aaggatggcg
     2701 cccgctggcg ggtggatgcc ctcacccttg atctggccgg caagccgcag gcggggcgca
     2761 agctgcgcgt gcgcatggtc ggccacgata ccctgtcggc gcgcaagcgg gtggtgggcg
     2821 ggttttatgc ctgggaacac gcgcaaaccc gtcgggatga aggcgaggtg tgtcaaggcg
     2881 tcagcgacag ccagggccgt tttgactgcc aggtgacggt gaagccgcac gagcaggtgg
     2941 aattcatcgc cgaggcagaa gacgaccgcg gccagcgcgc ccgcgccagc tattcggtgt
     3001 ggccggcctc cggcgaagac gcctggtttg acgcggccga ccacgaccgc atcgacatca
     3061 tccccgacaa aaccgaatac cagcccggcg agcgcgcccg ttttcaggtg cgcatgccgt
     3121 tccggcgcgc caccgcctgg gtggccatcg agcgtggcgg ggtgttggac acccgggtga
     3181 tcgagctgga tcgccccgag ttcgagctgg acatcgcccc ggactggaca ccgaatgtct
     3241 atgtgtcggt gctggcggtg cgcggccgtt tgcgtgaagt gccgtggtat tcgttttttg
     3301 cctggggctg gcgccgcccg aaagactggt gggatgcctt ctggcatgaa cgcccggact
     3361 acaccccggc cagtgcgctg gtggatttgt cgcggccggc agtcaagtac ggcattgccc
     3421 agattcgcgt gggcgaccgc gcccaccgcc tgagcgtgac ggtgaccccg gagcgcgagc
     3481 gctatggggt gcgccagacc gcacgggcgc gggtgcaggt gcgcctgccc gatggcagcc
     3541 cggcgccggc cggcagtgaa gtggcttttg ccgccgtgga cgaagccctg ctggaattgc
     3601 agcccaatgc cagttggaac ctgctgcgcg agatgtttcc cgactatggc taccgtggcg
     3661 aaatcgccac cagccagcaa caagtggtgg gcaagcgcca ttacggtcgc aaggcgttgc
     3721 cgccaggcgg tggcggcggc aaggcgccca cccgtcagct gctggatacc ctgctgacct
     3781 ggcaaccggc ggtcaagctg gatgcgcgcg gcgaagccat ggtggacatt ccggtgaatg
     3841 acgtgctcag ccgctttcgg ctggtggcgg tggccgatgt cggcgagcag ctgtttggcg
     3901 ccggcgaggc cagttttgag gtggcgcaag atgtgcaact ggtgtctggt ctgtcgccgc
     3961 tgacgcgtga aggcgatgcc atgacggcgg cggtgacggt gcgcaatggc agcaagcggc
     4021 cgctgacgct gacggtcagc gccagccgcg acagtcagac cctgcctgcc cagcggctga
     4081 ccctggctgc cggcgacgct cgccagctga gttgggcggt gacgaccccg gccggcgtca
     4141 gcgtccagca atggctgttt gccgcccgcg cggaagatgg ctcggccagc gaccggctga
     4201 gcctgcgcca gcgtgtcgag ccggccgtgc cggttagcgt ctggcagggg gcgtttattc
     4261 ggctggaggg cgagcaatcg ctgcctgtgg cgctgcctgc cggcgccttg cctggccagg
     4321 gtggcgtacg cgtcagcgtg cagtcgcggc tggcaggtga gctgcccggt gtggcgcgct
     4381 ggctggagga ctacccgttc agctgcctgg aacagcgcgc cgccgtggcg ctgggtcgcc
     4441 gcgatgctgc cgcctgggcc aagctgcaag aagacctgcc cggctacctg gacgagcacg
     4501 gcctggcgga ttattttccg cgcagcagtg ccaatgggcc atccggcagc gatgtgctca
     4561 ccagccatct gctcagcgtc agccacgaag ccggccagcc gctgcctgat gcgctgcgcg
     4621 agcgcatgct ggccgggctg tcggcctttg ttgaaggccg catcaagcgc gagctgcccg
     4681 gtggcgccgc cgggctggac gcgcgccggc tggctgccat gctggccttg cagcgccatg
     4741 gacgcttccg tccggccatg ctcgacgtgc tgagcgtaca gccacaaacc tggccaacgc
     4801 cgatgctggt gaactgggcc gagctgctgc tgcgccagcc ggccatcccc gctcgcgatg
     4861 cccgactggc acaggccagc cagctgctac gcgcgcgcct gttgtatcag ggcgcacggc
     4921 tggcgctggc gcgcgagcag caggacagcg cctggtggct gatggaagac ggcgatcttg
     4981 ccagcgcgcg cttgctgtgg ctggccagcc aactgccgga ttggcgcgac gatgcgccac
     5041 gtctggcggt gggtttgctg gcccgtcagc agggcggcca ctggcgcacc accaccgcca
     5101 atctgtgggg tgtgctggct acgcgggcgt tttcggcgca gttcgagcgt gtgccggttc
     5161 gcggtcagac cacggcggcg ctgggcgaag ccagggccac cgtcacgccg gcagaggcgc
     5221 cagtcagtgc gccgctgttg ccgtggccgg ccagcggtca gggcacgctg cggctgagcc
     5281 accagggcag cggtgcgccg tgggcgtcgg tgctggcgct ggcagcggtg aaatttgatg
     5341 gccaacgcag cgccggctac agcgtgcaaa aaaccctgac tccgctgagc cagcgcacgc
     5401 ctggccagta cagccctggt gatgtgctca aggtcacgct ggacatccgc gccgaagccg
     5461 gcatgggctg ggtggcgctg aacgatccga ttccagccgg cgccagcctg ctgggcagcg
     5521 gtctgggccg ggattcgcag attgccgtca gccaggcggg caacgacgac agcgtgacgc
     5581 caagcttcgt cgagcgccgc cacagcggtt atctggcgta tttccgccaa ctgccggctg
     5641 gcgtacaccg cctgagctac accctgcggc tgaaccaggc cggggtgttc cagttgccgc
     5701 ccacccgcat cgaggcaatg tacgcccctg agcaatacgg catgctggcc aatgcgccgc
     5761 tgacggtgac gcatgcgcgt taagacggga tggcgcgcgc tcgtgctggt ctgcgggctg
     5821 atgctggcca gcacagcgca ggcgacaccc agtctggccg aggtcaaggc cgcctggctg
     5881 gcgtcggatg tggtgtatgt cgatcgccat ggcgaagaaa tccagcgcct gcgccgcgac
     5941 aagcaggcgc gccggcagga ctgggtgagc ctggccgaga tgtcgccggc actgcgccat
     6001 gccctggtct tgtctgaaga tcgacgcttt taccagcata gtggcgtgga ttggtcgggg
     6061 gtggctgctg ccgcctgggc caatctgtgg aacacccgca cccgtggcgc ctcgacgctg
     6121 accatgcaac tggccggcct gctggacgcc gagctgaagg cgggcgctgg cgggcgcagc
     6181 gtctggcaga aactgggcca gggctggtcg gctgttgcgc tggagcgcca ttggcgcaag
     6241 gccgagattt tggaggcgta tctgaatctg gtgagctttc gcggcgagct ggtgggcgtg
     6301 ggcgcggtgt cgcgggcctt gttcgacaaa ctgcccgacg gtctgaaccg ccaggaagcc
     6361 gccatcctgg ccgccctgct gcgtggcccg aatgccgacg ctgccacgct cagccgccgc
     6421 gcctgcacgg tatatcaaga catgcaggct gacgaggccg tccaactgca tctgcccgca
     6481 ccagatgccg ccgccggcca gcgcgcctgc cagcaactga ccttgctgac tcagcaggcg
     6541 cttggccgtc ggcagggtgg ccaggcgctg accgtacaac tggccccgca ttttgcccgc
     6601 aagctggccg agcagcgccg cgacccggcc ggccgggtgg tgcgcactac gctggacgcc
     6661 cgcctgcaac ggctggctgc cgctgtcctg caccgccatc tggccagcct ggcccggcgt
     6721 catgtagaag acggcgcggt gctggtgctg gataacgcca gcggcgacat cctggcctgg
     6781 gtgggctcca gtggtgaatt gtccgccgcg cgcgaggtgg acggcgtcac cgcgccgcgc
     6841 caggccggct cgacgctcaa gccgtttttg tatcagctgg cgctgcaaca gcgctacctc
     6901 accgccgccg ccttgctgga cgacagtccg ctggacatgg ccaccgctgg cgggctgtat
     6961 tcgccgcaaa attattcccg tgatttcaaa ggcctggtgt cggtgcgcag tgcgctgggc
     7021 gcgtcgttga acattcctgc cgtgcgcacc gtcgcgatgg tcagcccgcg cctgttgcgt
     7081 gaccgcctgc acgcgctggg cctgggcacg ctgacccgtg acggtgattt ttatggctat
     7141 agtctggccc tgggcgcggc ggatgtgcgt ctgctggatt tgaccaatgc ctaccgcacg
     7201 ctggcgcagg gcggattggc cagcccgccg cgctggacgc tggatgcccc gccggccagg
     7261 ccggtgcgcc tgctggacgc ggcatccagc tttatcatcg ccgacattct ggccgaccgt
     7321 ggcgcgcgct cgcgcacctt cgggctggaa agcgccctgt ccacccgcta ttggagcgca
     7381 gtgaaaaccg gcaccagcaa ggacatgcgc gacaactggg cattgggctt cagccgccgc
     7441 tataccgttg gcgtgtgggt gggcaacgcc agcggccagc cgatgtggga tgtgtctggc
     7501 atgcacggtg cggcgccggt gtggctggat gtgctcaacg ccctgcacca gcacacgccg
     7561 tcgccaccgc ccgcgccgcc ggccggcgtg cagcgtcagc gcgtgcacta cgccgacgag
     7621 gtggaagccg agcgtgagga gtattttctg gcaggcagcg agcagcgcga gattgtcgcc
     7681 gtgcgcgcca gcgacagtca ggcgcctggc attgtttcgc cgctgcctgg cagtgtgtac
     7741 gccatcgacc cggacatccc acccaaacac cagcgcctgc tgctgcgcgc gcgcggcgtg
     7801 cccggtgcac aatggcgcat cgacggccgc cctgctggcc agggcgacgc cgtgccgtgg
     7861 tttccctggc ctggccgcca ccggctggcc ttgctgaatg cgcaaggcca gccggtgcaa
     7921 accctgactt ttgacgtgcg cggcgccgtg gccaagcctg gcgcccggcc gccgcgttga
     7981 agggccaata tggccgcgct gtggcccgcc acacgaccct gccggatgtt tcccctgtct
     8041 gccgtcgtgt gagcgtggtg cggatcacgc ggtttcaact gcgcctgcgc gccacgcgaa
     8101 agcggccgat gcgctcggcc agggcggcga tttcgccgcg cactgcggtc acgccggcat
     8161 tcaccgccgt caggcgat
//
LOCUS       DAOPYX010000126         8119 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_10597_length_8119_cov_10.583829, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000126 DAOPYX010000000
VERSION     DAOPYX010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 8119)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8119)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8119
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10597_length_8119_cov_10.583829"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            136..960
                     /locus_tag="PKN55_07715"
     CDS             136..960
                     /locus_tag="PKN55_07715"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNX71168.1"
                     /translation="MTTVATTAPAPIVAIAAPPAWRRLLTLARHALEASLIWLLLLAA
                     WLAAARYLPLGHNPLIPAPDVTLRALVESLPELWTGTLSSFSILLPGFGLACLLGIAS
                     GLAVGSSPRLQRMSLPFARVAAPVPPTVYIPYAIAVLPTFKLSAIFVVFIGAFWPIFQ
                     NSAAGAHALEARWRDNARILGLTGWAFQRQVVFPAALPHIFSGMAVGLGFSFILLTVA
                     ELFGANAGLGRFVQYYADFADYPKMVAGILYTGLITWLAMSALDAVKRRALFWVRA"
     gene            960..2003
                     /locus_tag="PKN55_07720"
     CDS             960..2003
                     /locus_tag="PKN55_07720"
                     /inference="COORDINATES: protein motif:HMM:NF014667.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HNX71169.1"
                     /translation="MRWLLMLCALAAAALAQAGALFAQDTLRLDQAREAAARAGKGVA
                     VWLTLPECAGCAEMASQVFTQPALAARLRQDFVEARIDLASPAPLTDLDGASTTAADF
                     ARRLRLYATPSLAFFAADGALRYRYTGVLNADGLRQLLDYVRRGDYDRQPFVPRQPAR
                     PKLHAAPPPVNAPQQPQFSLSDSAGQRRSLADWRGRVVALAVGYTHCPDVCPTTLSEL
                     KTAIEQLPAGLRGRVQGVFATLDPARDSAAILAEYAAAFRPRGGRAITALRGDGEADT
                     TALVRALQLHAERQPAADGADYTLDHSAGIYLFDARGRWLGVAAYQTPTAQLRADLLT
                     LARRAGAPHPDRF"
     gene            2007..3008
                     /locus_tag="PKN55_07725"
     CDS             2007..3008
                     /locus_tag="PKN55_07725"
                     /inference="COORDINATES: protein motif:HMM:NF013323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HNX71170.1"
                     /translation="MPAHRLISLAEAVRRYTRDGMQYASGAALPIGSDAIVFGRELLR
                     QRRSKLHAIFHCNSQQLNLLAAAGAVDKAECGFSGLEVFGFANGLRRAVESGQTALED
                     YSNLAMALRLLGGALNWPFVPATVNIGSDIQHRSAFAPEDYPATTKIPTVTDPFSGRE
                     IGAFSPLRPELAAIHVSLADPQGNAIMLGSEWSRFELSRAAGKVILIADAIVDGACML
                     QFPNLVRIPDILVEAVVYHPFAAWPQSSPGWYDADEAHMLHMNQALATAEGTAQYLNE
                     FVYGVNSLDDYLARIGDDVRARLSATATGFLLDPFRQWIKTPEQVAALLAHTPETHA"
     gene            3005..3811
                     /locus_tag="PKN55_07730"
     CDS             3005..3811
                     /locus_tag="PKN55_07730"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HNX71171.1"
                     /translation="MSELPYTRQEMMAIAAGRELRDGELAIFGVGLSMLAGYFAQAHH
                     APRLRSMTEGGVYGATPVGGLPWGIECNRLSANATSFTHALDALGFLVASGRCDVGII
                     GAAQVDKFGNVNTTGIWQDGLGEHYRAPDTRLTGAGGANDIACGAGRVVIMVSHEAKR
                     FAERVDYISSPGYLEGGNARARHGFVGGGPSAIVTTLGVLRPEPDSKEFVLTGYYPFS
                     SVAEIQAHTGWALRVAADVQEIAPPSPAELAALRRVDVTGALRKPAKHGK"
     gene            3857..4642
                     /locus_tag="PKN55_07735"
     CDS             3857..4642
                     /locus_tag="PKN55_07735"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: protein motif:HMM:NF006008.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HNX71172.1"
                     /translation="MNDTPYVLQDHQDGVVTLTLNRPQARNALSQGMLRALLAALADI
                     AANPDARVVVLAGAGPAFCAGHDLKELRAADYAPDYTEALFADCAELMQAIVALPKPV
                     IARVHGVATAAGAQLVASCDLALAAADARFATPGVNIGLFCSTPMVALSRNISHKHAM
                     QMLLTGELIDADTALRFGLINEHVPAAELEARTTALARQIASKSPLTLAMGKQAFYRQ
                     AELPLDEAYAYTRGVMVANLQARDAQEGINAFIDKRPPTWCGQ"
     gene            4885..6222
                     /locus_tag="PKN55_07740"
     CDS             4885..6222
                     /locus_tag="PKN55_07740"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HNX71173.1"
                     /translation="MSHHAADDRRYWDAALETQPRADWDALKLALLQDHLRLAYAQSP
                     FYRQHFDAAGVRPEQLRTLDDLRRFPFTHKHILRERQLAVPPFGDLLAAPERDIVYVS
                     ASSGSTGVPTASPFTARDFDDWMDYEARQFWSSGLRPHDRYCHALNFSLFVGGPCVLG
                     AQKLGALSIHAGTLPSERLLAVLRQFQATAMWTTPSYAWHLGETALKAGLDPRSELAV
                     RRIFVAGEPGGSIPETRQRIEALWGAKVYDYYGLSDIFGSCAGMCEEQDGLHWAEDHI
                     LAEVLDPETGQPVADGERGELVLTTLKKTARPLIRFRTGDIVSFTAEPCRCGRTSQRM
                     LGTHGRLDDMLIIKGVNIFPSDVEAVVRALAGFNGEYRLVVEREQHLDRLRVEAERAD
                     VSPHADGELAAQLARQLKAVTGVSAEVAVLAPDTLPRATHKAKRVEDRRQQVW"
     gene            complement(6257..>8119)
                     /locus_tag="PKN55_07745"
     CDS             complement(6257..>8119)
                     /locus_tag="PKN55_07745"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNX71174.1"
                     /translation="RLLVGAGITLLVLAGALVFSWRFTERMRAMVRQYQARLEQKNQE
                     LREHARKLQLAAAVFEGGSEAVVICDADNRILTVNRPFTQITGYAAEDVIGQSPSLFA
                     SGYHDAAFYQAMWLRLKQEDQWDGEIWNRRKDGSIFPEWLRIAVVRNAAGAIENYIAT
                     FSDISERKHAEEQIRHLAFYDPLTGLPNRRLLLDRLHQALLNSERHGRHAAVLFIDLD
                     NFKSLNDTQGHDVGDLLLVEVAARLKQCVRETDTVARLGGDEFVVMLENLSVEFAQAV
                     GEAERVATPIIQAMQAPFELAGYRHHSSCSIGVSVFFGCRESVEELLKHADTAMYQAK
                     AAGRGVARFFVPAMQEALEARLLWESELRRGIAEGQLRLFYQVQIGPGQRVRGVEALV
                     RWQHPERGLVPPGEFIALAEDTGLILPLGNWVLEEACRQLAVWAHSPATAHLHIAVNV
                     SARQFHQPGFAESVHGLIQRHGIHPSRLKLELTESLLLDNVNESIATMQALRTLGIAF
                     SMDDFGTGYSSLTYVKRLPIDQLKIDQSFVRDLTTDRDDEAIVRAIIAMAHSLKLEVI
                     AEGVETEAQRSFLEHNGCHAFQGYLFGRPMPIEQLDSWLASTQGRVSGAPGQAG"
BASE COUNT         1296 a         2789 c         2623 g         1411 t
ORIGIN      
        1 ttggcgagac aaagaaaagg ggtcgggtgc ggggcgaagc cccgccctca atcaaccttg
       61 gcatccctgc gcgtcagcgc agcctgcaac cccatttcga caaccctacc ccgaccgtga
      121 aaaaggacct ctcccatgac caccgtcgcc accaccgccc cggcgcccat tgtcgccatc
      181 gccgccccgc ccgcctggcg ccgcctgctc accctggcgc gccacgccct ggaagccagc
      241 ctgatctggc tgctgctgct cgccgcctgg ctggccgccg cccgctacct gccgctgggc
      301 cacaacccgc tgatccccgc gcccgacgtc accctgcgcg cgctggtgga atccttgccc
      361 gaactgtgga ccggcacgct gtcgtcgttt tccatcctgc tgcccggctt tggcctggcc
      421 tgcctgctgg gcatcgccag cggcctggcg gtgggcagct cgccgcgcct gcaacgcatg
      481 agcctgccct ttgcccgcgt cgccgcgcca gtgccgccca cggtgtacat cccctacgcc
      541 atcgccgtgc tgcccacctt caagctgtcg gcgatttttg tggtgttcat cggcgccttc
      601 tggccaatct tccagaacag cgccgccggc gcgcacgcgc tggaagcgcg ctggcgcgac
      661 aacgcccgca ttcttggcct caccggctgg gcttttcagc gtcaggtggt gtttcctgct
      721 gcgctgccgc atattttttc cggcatggcg gtcgggctgg gcttctcttt catcctgctc
      781 accgtggccg agctgtttgg cgccaacgcc ggcctgggcc gctttgtgca gtattacgcc
      841 gactttgccg actacccgaa aatggtcgcc ggcattctgt acaccggctt gatcacctgg
      901 ctggcgatgt cggcgctgga cgccgtcaag cgccgcgcct tgttctgggt gcgcgcctga
      961 tgcgctggct gctgatgctg tgcgcgctgg ccgccgccgc gctggcgcag gccggcgcgc
     1021 tgtttgccca agacaccctg cggctggatc aggcgcggga ggccgccgcg cgcgccggca
     1081 agggcgtggc ggtgtggctg accctgccgg agtgcgccgg ctgcgccgaa atggccagcc
     1141 aggtgttcac ccagccggcg ttggcggcgc ggctgcgtca ggattttgtc gaagcgcgca
     1201 ttgatctggc cagccccgcc ccgctgaccg acctggacgg cgcatccacc actgcggctg
     1261 attttgcccg ccgcctgcgg ctgtacgcca cgccgtcgct ggcgtttttt gccgccgatg
     1321 gcgcgctgcg ctaccgctac accggcgtgc tcaacgccga tggcctgcgc cagttgctgg
     1381 actatgtgcg ccggggcgat tacgaccgcc agccctttgt gccgcgccag ccggcgcgcc
     1441 ccaagctgca cgcagcgccg ccgccggtga atgccccaca gcagccgcag ttcagcctga
     1501 gcgacagcgc cggccagcgc cgttcgctgg ccgactggcg cggccgggtg gtggcgctgg
     1561 cggtgggcta cacccattgc ccggatgtct gccccaccac gctcagtgag ctgaaaaccg
     1621 ccatcgagca gctgccggcc ggcttgcgcg ggcgggtgca gggggtgttt gccaccctcg
     1681 atccggcgcg tgattcggcc gccatcctgg ccgaatacgc cgccgccttc cgcccacgcg
     1741 gcggccgcgc catcaccgcg ctgcgcggcg atggcgaagc cgacaccact gcgctggtgc
     1801 gcgcgctgca actgcacgcc gagcgccagc cggcggcgga cggcgctgac tacacactgg
     1861 accacagcgc cggcatttat ttgtttgacg cgcgcggccg ctggctgggc gtggcggctt
     1921 accaaacccc gacggcgcag ttgcgcgccg atctgctcac cctggcgcgc cgggccggcg
     1981 cgccgcatcc ggaccgtttt taaaccatgc ccgcccatcg tttgatttct ctggccgaag
     2041 ccgtgcgccg ctacacccgc gacggcatgc aatacgccag cggcgccgcg ctgcccatcg
     2101 gctcggacgc catcgtgttt ggccgcgaac tgctgcgcca gcgccgcagc aagctgcacg
     2161 ccatctttca ttgcaatagc cagcagctca atctgctggc cgccgctggc gcggtggaca
     2221 aggccgagtg cgggttttcc gggctggagg tgttcggctt tgccaacggc ctgcgccggg
     2281 cggtggaatc cggccagacc gcgctggagg attattccaa tctggccatg gcgctgcgcc
     2341 tgctgggcgg cgcgctgaac tggccatttg tgccggccac cgtcaatatc ggctcggaca
     2401 tccagcatcg ctcggccttc gcgcccgagg attatccagc caccacaaaa atcccgaccg
     2461 tcaccgaccc gttttccggg cgggaaatcg gcgccttcag cccgctgcgg cccgaattgg
     2521 cggccatcca tgtgtcgctg gccgacccgc agggcaacgc catcatgctg ggcagcgagt
     2581 ggagccgctt tgagctgtcg cgcgccgccg gcaaggtgat cctgattgcc gacgccatcg
     2641 ttgatggcgc ttgcatgctg caattcccca atctggtgcg cattcctgac attctggtcg
     2701 aggcggtggt ctatcaccca tttgccgcct ggccgcaaag ctcgcccggc tggtacgacg
     2761 ccgacgaggc gcacatgctg cacatgaatc aggcgctggc cacggccgaa ggcacggcgc
     2821 aatacctgaa tgaatttgtg tatggggtga acagcctgga cgactatctg gcgcgcattg
     2881 gcgacgatgt ccgcgcccgc ctgtcggcca ccgccaccgg ctttttgctc gacccgtttc
     2941 gccaatggat caaaaccccc gagcaagtgg cggcgctgct ggcgcacacc ccggagacgc
     3001 acgcatgagc gagctgcctt acacccgtca ggaaatgatg gcgattgccg ccggccgcga
     3061 gctgcgcgac ggcgagctgg ccatttttgg cgttggcctg tccatgctgg ccggctattt
     3121 tgcccaggcg caccacgcgc cccgactgcg ctcgatgacc gaaggcggcg tgtacggcgc
     3181 cacgccagtg ggcggtctgc cctggggcat tgaatgcaac cggctgtcgg ccaacgccac
     3241 cagttttacc catgcgctgg atgcgctggg ctttctggtg gcgtctggcc gttgcgatgt
     3301 ggggattatt ggcgcggcgc aggtggacaa gttcggcaac gtcaacacca ccggcatctg
     3361 gcaggatggc ctgggcgagc attaccgcgc gccagacacc cggctgaccg gcgcgggcgg
     3421 cgccaatgac attgcctgcg gcgccgggcg ggtggtgatc atggtcagcc atgaagccaa
     3481 gcgctttgcc gaacgggtgg actacatcag ctcgcccggc tatctggaag gcggcaacgc
     3541 ccgcgcccgc catggctttg tcggcggcgg cccatccgcc attgtcacca cgctgggcgt
     3601 gctgcgcccc gagccagaca gcaaggaatt tgtgctgacc ggctattatc cgttttccag
     3661 cgtggccgag attcaggcgc acaccggctg ggccttgcgc gtggcggccg atgtgcagga
     3721 aatcgccccg cccagcccgg ccgaactggc ggcgctgcgc cgggtggatg tcacgggcgc
     3781 cttgcgcaag ccagccaagc acggcaaatg accattggct atcatgcgga caccccgagg
     3841 agaacggaga acaaccatga acgacacccc ctatgtgctg caagaccacc aggatggcgt
     3901 ggtcaccctg acgctgaatc gcccgcaggc gcgcaatgcg ctgtcgcaag gcatgctgcg
     3961 cgcgctgctg gccgcgctgg ccgacatcgc cgccaacccg gacgcccgcg tggtggtgct
     4021 ggccggcgcc ggcccggctt tttgcgccgg ccatgacctg aaagaactgc gcgccgccga
     4081 ttacgcgccg gactacaccg aggcgctgtt tgccgactgt gccgagctga tgcaggcgat
     4141 tgtcgccctg cccaagccgg tgatcgcccg cgtgcacggc gtggccaccg ccgccggcgc
     4201 gcaattggtg gccagttgcg acctggcgct ggcggcggcg gacgcgcgct ttgccacccc
     4261 cggcgtgaac atcggcctgt tctgttccac gccgatggtg gcgctgtcgc gcaatatcag
     4321 ccacaagcac gccatgcaga tgctgctgac cggcgagctg atcgacgccg acaccgcgct
     4381 gcgctttggc ctgatcaacg aacatgtgcc ggccgccgag ctggaagcgc gcaccacggc
     4441 gctggcgcgg caaatcgcca gcaaatcgcc gttgaccctg gcgatgggca aacaggcgtt
     4501 ttaccgccag gcggaactgc cgctggatga agcctacgcc tacacccgcg gggtgatggt
     4561 ggccaatttg caggcccgcg acgcgcagga ggggattaat gcttttatcg acaagcgccc
     4621 gccgacgtgg tgcgggcagt aagcgtcgct cacaaaatgc agcaaagcgc gcctggcctg
     4681 gcgcgcggcg gcagacagta cacgcagtgc ggcaaggctg ggtttggtga gcggcctagc
     4741 ctgcgtcatt tcaagaacga gatttatccg gcgttctgag cccgctgtag tccgcttgga
     4801 tataagcgca taacccttgc ttcgttccgg ccgccggcgc ggcggccgac aatgccggct
     4861 ttgccttgct gccggagccc gccgatgagc caccacgccg ccgatgaccg ccgctattgg
     4921 gacgccgcgc tggaaaccca gccgcgcgcc gactgggacg cgctgaaact tgccttattg
     4981 caagaccacc tgcgcctggc ctacgcacag tcgccgtttt accgccagca cttcgacgcc
     5041 gcaggcgtgc gccccgagca attgcgcacg ctggatgacc tgcgccgctt tccgttcacc
     5101 cataagcaca tcctgcgcga gcgccagctg gcggttccgc cgtttggcga cttattggct
     5161 gcgcccgagc gcgacattgt gtatgtgtcg gcttccagcg gttcgaccgg cgtgcccacc
     5221 gcgtcgccat tcaccgcgcg cgattttgac gactggatgg attacgaggc acgccaattc
     5281 tggtcgtccg gcttgcgccc gcacgaccgt tattgccatg cgctgaattt ctcgctgttt
     5341 gtcggcggcc cgtgcgtgct gggcgcgcaa aagctggggg ccttgtccat ccacgccggc
     5401 acgctgccgt ccgagcggct gctggcggtg ctgcgccagt ttcaggccac ggcgatgtgg
     5461 accacgccgt cctacgcctg gcatctgggc gaaaccgcgc tgaaagccgg gctggaccca
     5521 cgcagcgagc tggcggtgcg gcggattttt gttgccggcg agccgggcgg ctcgatcccg
     5581 gaaacccgcc agcgcatcga ggcgctgtgg ggggccaagg tatacgatta ctacggcctg
     5641 tcggatattt ttggctcttg cgccggcatg tgcgaagagc aggacggcct gcactgggcg
     5701 gaagaccata ttctggcgga agtgctcgac ccggaaaccg gccagccggt ggccgatggc
     5761 gagcgcggcg agctggtgct gaccacgctg aaaaaaaccg cccgtccgtt gatccgcttt
     5821 cgcaccggcg atattgtttc gttcaccgcc gagccctgcc gctgcggacg cacctcgcaa
     5881 cgcatgctgg gcacccatgg ccggctggac gatatgctga tcatcaaggg agtgaatatc
     5941 ttccccagcg atgtcgaagc ggtggtgcgc gcgctggcgg gcttcaatgg cgaataccgg
     6001 ctggtggtgg agcgcgaaca gcatctggac cgcctgcggg tggaagccga gcgcgccgac
     6061 gtcagcccgc acgccgatgg cgaactggcg gcgcagctgg cgcgccagct gaaagccgtc
     6121 accggcgtgt cggccgaagt ggcggtgctg gcgccagaca ccctgccgcg cgccacccac
     6181 aaggccaagc gggtggaaga ccgccgccag caggtgtggt aagcgtcgtt taacaggctg
     6241 gcaagggcct ggcctactac cctgcctggc ccggcgcgcc gctcacccgc ccctgtgtgc
     6301 tggccagcca gctgtccagt tgctcgatcg gcatcgggcg gccaaacaga tagccctgga
     6361 aggcatggca gccgttgtgc tccagaaaac tgcgctgggc ttcggtttcc accccttcgg
     6421 cgatgacttc cagcttcagg ctgtgcgcca tggcgatgat ggcgcgcaca atggcctcgt
     6481 cgtcgcggtc ggtggtgaga tcgcgcacaa aggattggtc gattttcagt tggtcaatcg
     6541 gcaggcgctt gacgtaggtc agcgaggaat agccggtgcc aaaatcatcc atcgagaagg
     6601 caatgcccag cgtgcgcagc gcctgcatgg tggcgatgga ttcattgaca ttgtccagca
     6661 gcaggctttc ggtgagttcc agcttcagcc gcgacggatg aatgccgtgg cgctggatca
     6721 gcccgtgcac cgattcggca aagccaggct ggtgaaactg gcgggcgctg acattgacgg
     6781 caatatgcag gtgggcggtg gccggcgagt gggcccagac ggccagctgg cggcaggctt
     6841 cttccagcac ccagttcccc agcggcaaaa tcaggccggt gtcttcggcc agggcaataa
     6901 actcgcccgg cggcaccagc ccacgctcgg ggtgctgcca gcgcaccagc gcctccacgc
     6961 cgcgcacgcg ctggccgggg ccgatctgca cctgataaaa caagcgcaac tggccttcgg
     7021 caatgccccg gcgcagttcg gactcccaga gcagccgcgc ttccagcgcc tcttgcatgg
     7081 ccggcacaaa aaagcgcgcc acgccgcgcc cggccgcctt ggcctgatac atggcggtat
     7141 cggcgtgctt gagcagttct tccacacttt cccggcagcc aaaaaacacg ctgacgccaa
     7201 tactgcacga gctgtgatgc cgataaccgg ccagctcgaa cggcgcttgc atggcctgga
     7261 taatcggcgt ggccacccgc tcggcctcgc ccaccgcctg ggcaaattca acggacaggt
     7321 tttccagcat caccacaaat tcatcgccgc ccaagcgcgc cacggtgtcg gtttcgcgca
     7381 cgcactgctt gagccgcgcc gccacttcca ccagcagcag gtcgcccaca tcgtggccct
     7441 gggtgtcgtt cagcgatttg aaattgtcca ggtcgataaa caacactgcg gcgtggcggc
     7501 cgtggcgttc gctattgagc agcgcctggt gcaggcggtc cagcagcaga cggcggttgg
     7561 gcagaccggt gagcggatcg taaaaggcca ggtggcggat ttgctcttcg gcgtgtttgc
     7621 gctcggagat atccgagaag gtggcaatgt agttttcgat tgcgccagcg gcattgcgca
     7681 ccacggcaat gcgcagccat tccgggaaga tcgacccatc cttgcgccgg ttccagatct
     7741 cgccatccca ctggtcttcc tgcttcagcc gcagccacat ggcctgataa aacgcggcat
     7801 cgtggtagcc cgaggcaaac agcgacgggc tctggccaat cacgtcttcg gcggcatagc
     7861 cggtaatctg ggtgaatggc cggttgacgg tgagaatgcg gttatccgca tcgcagatca
     7921 ctaccgcctc gctgccgccc tcgaacaccg cagccgccaa ctgcaacttg cgggcgtgct
     7981 cgcgcagttc ctgatttttt tgctccagcc gcgcctgata ctggcgcacc atggcgcgca
     8041 tgcgctcggt aaagcgccag gaaaacacca gcgccccggc cagcaccaac agggtgatgc
     8101 cggcccccac cagcagccg
//
LOCUS       DAOPYX010000127         8063 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_10699_length_8063_cov_11.857143, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000127 DAOPYX010000000
VERSION     DAOPYX010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 8063)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8063)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8063
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10699_length_8063_cov_11.857143"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(5..>275)
                     /locus_tag="PKN55_07750"
     CDS             complement(5..>275)
                     /locus_tag="PKN55_07750"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008952468.1"
                     /note="Catalyzes two discrete reactions in the de novo
                     synthesis of purines: the cleavage of adenylosuccinate and
                     succinylaminoimidazole carboxamide ribotide; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HNX71175.1"
                     /translation="EANPAALAADLDATWELLAEPIQTVMRRYGIEKPYEQLKDLTRG
                     KGGITQATLAAFIQNLAIPEDEKTRLLALSPATYLGKAAELAKRI"
     assembly_gap    276..285
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(296..1462)
                     /gene="purB"
                     /locus_tag="PKN55_07755"
     CDS             complement(296..1462)
                     /gene="purB"
                     /locus_tag="PKN55_07755"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF006764.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HNX71176.1"
                     /translation="MTLHVLTALSPLDGRYAGQAEALRPVFSEYGLMKARIQVELEWL
                     KMLAAEPGIAEVAPFSPATIEEIDAVIAGFSVEHAEAIKAIEARTNHDVKAIEYWLKE
                     TLSGNAEVMAASEFIHFACTSEDINNLSHALMLKAAREQVLLPKLDEVVAKLTELAHV
                     HADLPMMSRTHGQPATPTTLGKEMANVVYRLKRQREQIAAQPLLGKINGAVGNFNAHL
                     SAYPNVGWEGLATRFVEGLGLAWNPYTIQIEPHDYMAELFQAMSRVNTILIDLDRDVW
                     GYISLGYFKQKVKEGEVGSSTMPHKVNPIDFENSEGNLGIANALLGHLAEKLPLSRWQ
                     RDLTDSTVLRNMGVAFGYTQLGFTALLRGLNKLEANPAALAGWLPACSGRAAAR"
     gene            complement(1527..2423)
                     /locus_tag="PKN55_07760"
     CDS             complement(1527..2423)
                     /locus_tag="PKN55_07760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014088357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX71177.1"
                     /translation="MYDIAVLTDGRYVAPDDNDWYIRQIHQEDGLVADALRRQGLSVV
                     RRDWADPDVDWSDCRALLFRTTWDYFDRFAEFSPWLEQVSGQCRLFNEAALIRWNIDK
                     HYLGDLAAAGVNITPTWFIEPGDARTLAEHVAACGWPELILKPAVSGAARHTYRFAAG
                     DAGQHQAVFADLIAVEAMLLQPFQQSVLEQGEISLMVMDGRVSHAIRKTPKANDFRVQ
                     DDHGGTVHAHEASAEEIAFAEAAVAAVPFDVIYARVDVIRDNAGQLAIMELEMVEPEL
                     FFRFHPAAADQLAAGIARRLAA"
     gene            complement(2696..4054)
                     /gene="ffh"
                     /locus_tag="PKN55_07765"
     CDS             complement(2696..4054)
                     /gene="ffh"
                     /locus_tag="PKN55_07765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HNX71178.1"
                     /translation="MLDNLTTRLSGVIKNLRGHARLTEDNIKDAMREVRMALLEADVA
                     LPVVKTFINDVRERAMGHEVMGSLTPGQAVVGVVNDELTRLMGDKNDALNLAAVPPAI
                     VLMAGLQGAGKTTTSGKLAKLLKEQNKKKVLLVSADVYRPAAIEQLKLLAQQLDVEWF
                     PSDASQKPVDIARAALDHAKRHFHDVLIVDTAGRLAIDTAMMEEIKALHAALNPVETL
                     FVVDAMQGQDAVNTAQAFNEALPLTGVILTKLDGDSRGGAALSVRHVTGKPIKFIGVG
                     EKLTGLEPFHPERMASRILGMGDVLSLIEDVQKGIDQDEAQKMMKKMKSGKGFDLEDF
                     KAQIQQMKKMGGMASLMEKMPGQLGQMAKGLEGMQADKAVGRIEGIINSMTPTERRKP
                     ELIKASRKRRIAAGAGVTVQEVNRLLAQFEQTQKMMKQFSKGGLSKLMRGMKGMMGGG
                     GL"
     gene            4212..5036
                     /gene="ccsA"
                     /locus_tag="PKN55_07770"
     CDS             4212..5036
                     /gene="ccsA"
                     /locus_tag="PKN55_07770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011137379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HNX71179.1"
                     /translation="MTFPFASLTAWLALAAYAGLSWHFLAHAHGWSIPWRRALNIEHL
                     LVAAALALHFLALLPANAHGLAFGLGQSLSTVMWLTVLIYWTASFFYKLDGLQTCLMP
                     MAVLGVVLGLALPPAYLAYDFHQPALLLHIAVSLLAYSLLLIAAILAVMMLVLDRALH
                     AKRFSPVIRQMPPLLALERLLFQVLWAGFALLTLTVFSGVLFSEQLFGRPVALSHKTV
                     FALMSWLIFAALMWGRWRHGWRGRLAVRWTLAGFVLLLLAYVGSKFVLEVLLHRMG"
     gene            5165..5539
                     /locus_tag="PKN55_07775"
     CDS             5165..5539
                     /locus_tag="PKN55_07775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008952472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEA family DNA-binding protein"
                     /protein_id="HNX71180.1"
                     /translation="MKKLLVMLASALTFACGAAFAAVDLNTASQQELESVKGIGPAKA
                     KAILDYRTKNGPFKAVDDLAKVPGFGAKSVSNMKAELAVGAAKPAAAPAAAKPAAPAA
                     PAAKPAAPAAPAAKPAEPAKKP"
     gene            complement(5710..6765)
                     /locus_tag="PKN55_07780"
     CDS             complement(5710..6765)
                     /locus_tag="PKN55_07780"
                     /inference="COORDINATES: protein motif:HMM:NF024039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HNX71181.1"
                     /translation="MPTDTPPSTSALIPPAERASIAMVFVREALAAWRARGVDCAPLL
                     LAAGIAPASLADDGARVSPAQFGALWRQMAASLDDEFFGLADRPMKAGSFTLLCHSVL
                     HAGVLQRALLRMLRFLRVTLDGLEGELRVSGEQACIVLRDLPAPGEASAPPRRAFAYG
                     AYWLMVCGVASWLTGRRLPLTAVDFPGPEPAFSPAWRAVFCPQLNFEQPVAALYFPAQ
                     ALHWPLLRDEAALKSFLRQAPANFLALRPARDGLAARIHRQLRTTPPAAWPDFASLAR
                     QLHLSPATLRRRLAQEGQSYRRVQEAARQALAMTLLANRALSLAEVASALGFAETSAF
                     CRAFKRWTGQRPGDCRP"
     gene            6966..>8063
                     /locus_tag="PKN55_07785"
     CDS             6966..>8063
                     /locus_tag="PKN55_07785"
                     /inference="COORDINATES: protein motif:HMM:NF014564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HNX71182.1"
                     /translation="MPQSASTPPAGAGPLHGVRVVEFAALGPAPMAAMLLADLGAEVV
                     RIERKTAAGRPVAELFDPQRDILNRSRHTLALDLKSPADIDAALAVIARADVLIEGFR
                     PGVMERLGLGSEVCLARQPALVYGRMTGWGQHGALAPAVGHDINYLALSGALHSIGGA
                     GQPMPPLNLVADCGGGAMLLALGVLAARLHASQTGRGQVVDAAMTDGSALLMSMIYTL
                     HAMGEWRGQREANLLDGGAHFYGTYCCADGKWLAVGAIEPQFYAALLKALGIDDPAFQ
                     AQWDRAHWPALRAKLAQVFAQKPRDAWCALLEGSDACVSPVLDLDEAPRHAHNQSRQT
                     FIELGGVTQPAPAPRFSHTPSPTPRPPGVGDE"
BASE COUNT         1276 a         2621 c         2618 g         1538 t
ORIGIN      
        1 gcgctcagat gcgcttggcc agctccgctg ccttgcccag atacgtggcc ggcgacagcg
       61 ccagcagacg ggttttttcg tcttccggaa tcgccaggtt ctggataaag gccgccagcg
      121 tggcctgggt gatgccgccc ttgccgcggg tcaggtcttt cagctgttcg tacggcttct
      181 caatgccgta gcggcgcatc accgtctgga tcggctcggc cagcagttcc caggtggcgt
      241 ccaggtcggc ggccagtgcc gccgggttgg cttccnnnnn nnnnncagct gggcttcacc
      301 gcgctgctgc gcggcctgaa caagctggaa gccaacccgc cagcgctgcc gggttggctt
      361 ccagcttgtt caggccgcgc agcagcgcgg tgaagcccag ctgggtgtag ccaaacgcca
      421 cgcccatatt gcgcagcacg gtggagtcgg tcaggtcacg ctgccagcgg ctgagcggca
      481 gtttttccgc cagatggccc agcagcgcat tggcgatgcc cagattgcct tccgagtttt
      541 caaagtcgat cgggttgacc ttgtgcggca tggtcgagct gcccacttcg ccttccttga
      601 ccttctgctt gaagtaaccc agcgagatat agccccacac gtcgcggtcc aggtcgatca
      661 ggatggtgtt cacccggctc atcgcctgga acagctcggc catgtaatcg tgcggctcga
      721 tctggatggt gtacgggttc caggccaggc ccaggccttc gacaaagcgg gtggccaggc
      781 cttcccagcc cacgttcggg taggcgctca ggtgggcgtt gaagttgccc accgcgccgt
      841 tgatcttgcc cagcagcggc tgtgcggcga tttgctcgcg ctggcgcttc aggcggtaca
      901 ccacattggc catttccttg cccagcgtgg tcggcgtggc cggttggccg tgggtgcggc
      961 tcatcatcgg caggtcggcg tgcacatggg ccagctcggt cagcttggcg accacttcat
     1021 ccagcttggg cagcaacacc tgttcgcggg cggctttgag catcagcgcg tgcgacaggt
     1081 tgttgatgtc ttccgaggtg caggcaaagt ggatgaactc gctggccgcc atcacttcgg
     1141 cgttgccgga cagggtttct ttcagccagt attcaatggc cttcacatcg tggttggtgc
     1201 gcgcctcgat ggccttgatg gcttcggcgt gctcgacgct gaagccggca atcacggcgt
     1261 cgatttcttc aatggtggcc ggcgagaacg gcgccacttc ggcaatgccc ggctcggcgg
     1321 ccagcatctt cagccattcc agctccacct ggatgcgggc cttcatcagg ccatattcag
     1381 aaaacaccgg gcgcagggct tcggcttggc cggcgtaacg gccgtccagc ggcgacaggg
     1441 cggtgagcac atgcaaggtc atcagcacat tcctaacaaa atcggcgacg gcgcgcggcc
     1501 agtcgccagg ggtgagtcaa tacggttcag gcggccagcc ggcgggcaat gccagcggcc
     1561 agttgatcgg cggcggccgg gtgaaagcgg aagaacaact ccggctcgac catttccagc
     1621 tccatgatgg ccagttggcc ggcgttgtcg cggatcacgt ccacacgggc gtagatcacg
     1681 tcaaacggca cggcggccac cgccgcctcg gcaaaggcga tttcttcggc actggcctca
     1741 tgggcgtgca cggttccgcc gtggtcgtcc tgcacgcgaa aatcattggc cttgggcgtt
     1801 ttgcggatgg cgtggctgac gcggccgtcc atcaccatca gcgagatttc gccctgttcc
     1861 agcacgcttt gctgaaacgg ctgcaacagc atcgcctcga cggcgatcaa gtcggcaaac
     1921 accgcctgat gctgcccggc gtcgcctgcg gcaaagcggt aggtgtggcg cgccgcgcca
     1981 gacactgccg gcttgagaat cagctctggc cagccgcagg cggccacatg ctcggccagc
     2041 gtgcgcgcat cgcccggctc gataaaccag gtcggggtga tgttgacgcc agcggcggcc
     2101 aggtcgccca gataatgttt gtcgatgttc cagcggatca gcgccgcttc gttgaacagc
     2161 cggcactggc cggacacctg ttccagccac ggcgaaaact cggcaaaacg gtcgaaataa
     2221 tcccaggtgg tgcgaaacag cagggcgcgg cagtcgctcc agtccacgtc cgggtcggcc
     2281 cagtcgcggc gcaccacgct caagccctgg cggcgcaagg cgtcggccac caggccgtct
     2341 tcttgatgaa tctggcggat gtaccagtca ttgtcatccg gcgccacata gcggccgtcg
     2401 gtcagcacgg caatatcgta catgcaaaaa tctcttgaaa aaacagtcgc cagcccggcc
     2461 atgcggcggg gctggcgcat catgcgtaag tgggctggcg gtgcgtcagc cctgtacagc
     2521 gtttggtccg gcgtgcgaca agactgcgac aacgccagga ggcctgcgtt agccgcgcta
     2581 aaagagccgc tcacaaaatt cagcgcagcg gtcctggcta ggcgcacgac cgcagacagt
     2641 acaagtcgta cggcaaggtc gggcaacgac gccaggaggg ttttgtgagc ggctcttaca
     2701 gtccgccgcc gcccatcatg cccttcatgc cgcgcatcag cttggacagc ccgcccttgg
     2761 agaactgctt catcatcttt tgcgtttgtt caaactgcgc caggaggcgg ttgacctctt
     2821 gcacggtcac cccagcgcca gcggcgatgc ggcgtttgcg gctggccttg atcagctcgg
     2881 gcttgcgccg ctcggttggc gtcatcgaat tgatgatgcc ctcaatgcgg cccaccgcct
     2941 tgtcagcctg catgccttcc aggcccttgg ccatctggcc cagctggccg ggcatttttt
     3001 ccatcaggct ggccatgccg cccatttttt tcatctgctg gatttgcgcc ttgaagtctt
     3061 ccagatcgaa gcccttgccg gacttcatct tcttcatcat cttctgcgct tcgtcctgat
     3121 cgatgccttt ttgcacatct tcgatcagcg acagcacatc gcccatgccc agaatccggc
     3181 tggccatccg ctccgggtgg aagggctcca ggccggtgag tttttcgccc acgccgatga
     3241 atttgatcgg cttgccggtg acatggcgca ccgacagcgc cgcgccgccg cgcgaatcgc
     3301 cgtccagctt ggtcaggatc accccggtca gcggcagcgc ctcgttaaag gcctgggcgg
     3361 tgttcaccgc atcctggccc tgcatggcgt ccaccacaaa cagggtttcc accgggttca
     3421 gcgccgcgtg cagggctttg atttcttcca tcatcgccgt gtcgatggcc agacggccgg
     3481 cggtatcgac aatcagcaca tcatgaaaat gccgcttggc gtggtccagc gcggcgcggg
     3541 cgatgtccac cggcttctgg ctggcgtcgg acggaaacca ttccacgtca agctgttgcg
     3601 ccaacagctt gagctgctcg atggcggccg ggcgatacac gtcggccgac accagcagga
     3661 cttttttctt gttttgttct ttcagcagct tggccagctt gcccgaggtg gtggttttac
     3721 ccgccccctg caaaccggcc atcagcacga tggccggcgg cacggcggcc aggttcagcg
     3781 cgtcgttttt gtcgcccatc agacgggtga gttcgtcgtt gaccacgccc accaccgcct
     3841 ggcccggggt gaggctgccc atcacctcgt ggcccatggc gcgttcgcgc acgtcgttga
     3901 tgaacgtctt caccaccggc agcgccacat cggcttccag cagcgccatg cgcacttcac
     3961 gcatggcgtc cttgatattg tcctcggtca gccgggcgtg gccgcgcaag ttcttgatca
     4021 cgccggagag gcgtgtggtg agattgtcca gcatgggtgt gtcaggctcc gcctagcaaa
     4081 gtcaggattg acgccgcgca gcgagtccac gcgcgccaat gtcgaaacgg tttggtgtat
     4141 gctttgacca aaccgtgatt ttacaacagc cgggggcttt gcgcccgctg cgcttgacag
     4201 acccttgacc catgacattt ccgttcgcct ccctgaccgc ctggctggcg ctggccgcct
     4261 acgccggcct gtcctggcat ttcctggctc acgcccatgg ctggagcatt ccctggcggc
     4321 gcgcgctcaa tatcgaacac ctgctggtgg ccgccgcgct ggcgctgcat tttctggcgc
     4381 tgctgcccgc caacgcccat ggcctggcgt ttggcctggg ccagagcctg tccacggtga
     4441 tgtggctcac cgtgctgatt tactggaccg ccagcttttt ttacaagctc gacggcctgc
     4501 aaacctgcct gatgccgatg gcggtgctgg gcgtggtgct ggggttggcg ttgccgccgg
     4561 cctatctggc ctatgatttt caccagccgg ccttgctgct acacattgcc gtgtccttgt
     4621 tggcctacag cctgttgctg attgccgcga ttttggcggt gatgatgctg gtgctggatc
     4681 gggcgctgca cgccaagcgc ttttcgccgg tgattcgcca gatgccgccc ttgctggcgc
     4741 tggagcgcct gctgtttcag gtgctgtggg ccggctttgc cctgctgacg ctgacggtgt
     4801 tttccggggt gttgttttct gagcagttgt ttggccgacc ggtggcgctg tcgcacaaaa
     4861 ccgtgtttgc gctgatgtcg tggctgattt ttgcggcgtt gatgtggggg cgctggcggc
     4921 atggctggcg cggccgcctg gcggtgcgtt ggacgctggc cggttttgtg ttgctgttgc
     4981 tggcttatgt gggcagcaaa ttcgtgctgg aagtgctgct gcaccgtatg gggtgatctt
     5041 ggcgcaccga ttttgcgtaa agaccaccat tgcatccgcc ctgctggcca tgctagcgtc
     5101 ttgatgggcg gcatgcccat acctgatcac aatgattact tgccgaatcg aggagaaccc
     5161 cctgatgaaa aaactgcttg tcatgcttgc cagcgcgctg acctttgcct gcggcgcggc
     5221 ctttgccgct gtggatttga acaccgccag ccagcaagag ctggaaagcg tcaagggcat
     5281 cggcccggcc aaggccaagg ccattctcga ctatcgcacc aagaatggtc cgttcaaggc
     5341 cgtggatgat ctggccaagg tgccgggttt tggcgccaag tcggtttcca atatgaaggc
     5401 agaactggcc gtgggcgccg ccaaacctgc tgctgccccg gccgccgcca agcctgccgc
     5461 acctgctgcg cctgccgcca aacccgctgc gccggcagcg ccggccgcca agccggctga
     5521 accggcgaaa aagccgtaat cgttgcttga ctggcccatg caaaacgccc gcagctgcgg
     5581 gcgttttgca tggtgggtgg attagcatcg ctgacaaaac ccagcgaagc ggctctggtt
     5641 aggtgtgtga gcacagacag tgcaagcagc acggcaaggt cacacaacaa cgccaggggg
     5701 gagccgctct taagggcggc aatcgccggg gcgctggcct gtccagcgtt tgaacgcccg
     5761 gcaaaacgcg ctggtttcgg caaagcccag cgcgctggcc acctcggcca ggctgagcgc
     5821 gcgattggcc agcagcgtca tcgccagcgc ctggcgcgcc gcctcttgca cgcgccggta
     5881 gctttgcccc tcctgcgcca gccgccggcg cagggtggcc ggcgacaggt gcagctggcg
     5941 cgccaggctg gcaaagtccg gccaggcggc gggcggggtg gttcgcaatt gccggtggat
     6001 gcgcgcggcc aggccatcgc gcgccgggcg cagcgccaga aaattggccg gggcctgacg
     6061 caaaaagctt ttcagcgcgg cctcgtcgcg cagcagcggc cagtgcagcg cctgcgccgg
     6121 aaaatacagc gccgccaccg gctgctcaaa attcaactgc gggcaaaaca ccgcgcgcca
     6181 tgccgggctg aacgccggct ccgggcccgg aaaatccacc gccgtcagcg gcagccggcg
     6241 gccggtcagc caactggcca ccccgcacac catcagccag tacgcgccat aggcaaacgc
     6301 ccgccgtggc ggcgcgctgg cctcgcccgg cgccggcagg tcgcgcagca caatgcaggc
     6361 ttgttcgccg ctgacgcgca attcgccttc cagcccatcc agcgtcaccc gcaaaaagcg
     6421 cagcatgcgc agcaaggcgc gctgcaatac gccggcgtgc agcacgctgt ggcacaacaa
     6481 ggtaaaactg ccggccttca tcggccggtc ggccaggcca aaaaattcat cgtccagact
     6541 ggcggccatc tgccgccaca gcgcgccaaa ctgcgccggg ctgacccgtg cgccatcgtc
     6601 tgccaggctg gccggggcaa tgccggccgc cagcaataag ggcgcgcaat ccaccccacg
     6661 cgcgcgccag gcggccagcg cttcgcgcac aaacaccatg gcgatgctgg cccgttcggc
     6721 gggcgggatg agggcgctgg tgctgggtgg cgtgtcggtt ggcattggcc gattttaaca
     6781 ggcaaattga gcgtttccgg catcgcccgc catgggcgtc tgggcctaca gtggcgagct
     6841 ttagcgttgc tcacaacacg cgcctggcgt ggttgcgcgg ccttgccgtt gtttgcgcgc
     6901 gcctcgccag gcgcgctgcg ctgagtgtgg tgagctgcgc ttattcccgc ttcaggaggt
     6961 gcatcatgcc gcagtctgct tctactcccc cggccggcgc cggccctttg cacggcgtgc
     7021 gcgtggtcga gtttgccgcg ctgggcccgg cgccgatggc ggccatgctg ctggctgatc
     7081 tgggcgccga ggtggtgcgc atcgagcgca aaaccgccgc cgggcggccg gtggccgagc
     7141 tgtttgaccc acagcgcgat attctcaacc gctcgcgcca caccctggcg ctggatttga
     7201 aatccccggc cgacatcgac gccgcgctgg cggtgatcgc ccgcgccgat gtgctgatcg
     7261 agggctttcg ccccggcgtg atggagcgcc tgggtctggg gtccgaggtg tgtctggcgc
     7321 gccagccggc gctggtgtac gggcggatga ccggctgggg tcagcacggc gcgctggccc
     7381 ccgccgtggg gcacgacatc aattatctgg cgctgtctgg cgcgctgcac agcattggcg
     7441 gcgccggcca gccgatgccg ccgctgaacc tggtggccga ttgcggcggc ggcgccatgc
     7501 tcctggcgct gggcgtgctg gccgcgcggc tgcacgccag ccagaccggc cgtggccagg
     7561 tggtggacgc ggcgatgacc gatggctcgg ccttgctgat gagcatgatc tacaccctgc
     7621 acgccatggg cgaatggcgc ggccagcgcg aggccaatct gctggacggc ggcgcgcatt
     7681 tttacggcac gtattgttgc gccgacggca aatggctggc ggtgggggcg attgagccgc
     7741 agttttacgc cgcgctgctc aaggcgctgg gcatcgacga cccggcgttt caggcgcaat
     7801 gggatcgcgc ccactggccg gcgctgcgcg ccaaactggc gcaggtgttt gcgcaaaaac
     7861 cgcgcgacgc ctggtgtgcg ctgctggaag gcagcgacgc ctgcgtgtcg ccagtgctgg
     7921 atctggacga agcgccgcgc catgcgcata accagtcgcg gcagaccttt attgaactgg
     7981 gcggcgtcac ccagccggcg ccagcgccgc gctttagcca caccccgtcg cccaccccac
     8041 gcccgcccgg cgtgggcgat gag
//
LOCUS       DAOPYX010000128         8053 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_10719_length_8053_cov_5.414104, whole genome shotgun sequence.
ACCESSION   DAOPYX010000128 DAOPYX010000000
VERSION     DAOPYX010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 8053)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8053)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8053
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10719_length_8053_cov_5.414104"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            50..931
                     /gene="rfbA"
                     /locus_tag="PKN55_07790"
     CDS             50..931
                     /gene="rfbA"
                     /locus_tag="PKN55_07790"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014088373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HNX71183.1"
                     /translation="MTRKGIILAGGSGTRLYPATLAVSKQLLPVYDKPMIYYPLSTLL
                     LAGIQDILIISTPQDTPRFAQLLGDGSQWGISLSYAVQPSPDGLAQAFIIGESFLNGA
                     PSSLVLGDNIFYGHALVELLGRAAAKPSGASVFAYQVNDPERYGVVEFDAEGRALSLE
                     EKPAQPKSRFAVTGLYFYDEQVVDIAKQIQPSPRGELEITDVNRVYLEQGQLDVQLMR
                     RGYAWLDTGTHDSMLEAGQFIQTLEKRQGFKVACPEEIVWRQGWIDDAQLARLAAPLA
                     KVGYGLYLNQLLAESNP"
     gene            1011..2126
                     /locus_tag="PKN55_07795"
     CDS             1011..2126
                     /locus_tag="PKN55_07795"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019922990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HNX71184.1"
                     /translation="MSSPIPFLDLKAINQRHRDALLQACERVIDSGWYVLGGEVSAFE
                     AAFAQYCEAPHCVGVANGLDALTLILLADGIGPGDEVLVPSNTYIATWLAVSAVGATP
                     VPVEPDPLTYNIDPVAAAAAITSRTKAILAVHLYGQPADMQALAALAQAHGLRLYEDA
                     AQGHGARCHGRRAGSLSDAAGFSFYPGKNLGALGDGGAVVTRDAALADRVRVLANYGS
                     HIKYHNLVRGVNSRLDTLQAALLMTKLPSLDADNAHRHTVAQRYLDGLAACPLQLPHT
                     PAWAESVWHVFVVSAPQRDALAAALKAADIGSLIHYPIPPHLQPAYADLGIPAGALPI
                     AERMAEQVLSLPMGPTLSLQEVDRVIAAIRQHFGVGA"
     gene            2126..3397
                     /locus_tag="PKN55_07800"
     CDS             2126..3397
                     /locus_tag="PKN55_07800"
                     /inference="COORDINATES: protein motif:HMM:NF014051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HNX71185.1"
                     /translation="MARPRLVALIAGTSGAKLYSMGLNILALALTARWLGPDGRGMLA
                     TLTTWVNTAAMLASLSLGSVAMQRLAGCRDSARFGDVFHALLRFAWILAICAWGVMAL
                     LWLARGSALFGNAPAWALALACLMLPCLVWEGYANALLTTIERLRVFNLSLVTGRSLM
                     LLGLLCLAVLDGVTLAGVLAVSVLAQCWIAGYSFPTLRAWARQHQAAGASTAEGRALL
                     HGGMRLHLNTVGTLALNMAGTLLIANMLGAREAGFFQLAQQLMTVLLVIPQSASLVIY
                     GRQGELGPDGVWPLQRKMLLGVVALTAIAGLAMAMSAHIWLPWVAGPGYEPAIPLLLA
                     MLPILVMLSFSSMMASQWIGRGFFLTASLLTVLVGLVNLLASSVWIPEFGMMGAVYAQ
                     LLALLVAVFTNSSMALWCELKSRRLRAESGK"
     gene            3394..4476
                     /locus_tag="PKN55_07805"
     CDS             3394..4476
                     /locus_tag="PKN55_07805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX71186.1"
                     /translation="MKVLLLGEFSALHRDLCDGLLALGHDARVASTGDGWKAIPTDIM
                     LGSNASGLKGRLARLWSPLASLPKLINHDVIQFINPVLFSTQFGYNEKLIRMLFAQNN
                     KSFLVAAGCDAFYWNEGRQRLPYGPFADFEQQDLGGNSSPWRSPVRRKFNQWLAENVD
                     GIIPIMYDYWVGYQGFPNLCSPLPIPVNLEKIKYQENTLKQGKLVVFHGLNREGFKGT
                     GYVRKAFDILSNKYSGEVECVIQGRMPLKDYMKLLSTVNVVVDQTSSHGSGVNGLFSL
                     ALGKVVLGGNEPAHQALMYGDDPCPIRNISQDPMSIVAQVEQIMAQRDQLAAMGQAGR
                     EFIMRHHDHRRVAARYVDTWQMESKK"
     gene            4473..5615
                     /locus_tag="PKN55_07810"
     CDS             4473..5615
                     /locus_tag="PKN55_07810"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNX71187.1"
                     /translation="MSASLDVLFLIRSLHIGGAERQLALLARTMHASGHRVRVATFYA
                     SGAFEADLRQAGVPVLDLKKSGRWDVLTFFWRLVRLLRKEKPVIVHGYLPMSNLLALL
                     AKPFAGWPKVVWGIRSSGSDPRHYGWMGRLEAWLQARLSRWADLIICNSQRGRQYALS
                     QGFPLARTVCVPNGVNCEQFFPDPAAGLRTREAWGISQDVYLIGIVGRFVPLKNHAGL
                     LRAAAPLLLTQPCAVVCIGSGSSQAIQEMRALAVELGIADKVFFPGAHLEMCAAYNAL
                     DLFVSASLTEGAPNVLGEAMACNLPVICTDVGDAAWLAADYGQVVSPGDVPALERAIL
                     HAYACRKIPRASPRERVLSAFSVMALAANTETLLRNVCTASETKTP"
     gene            5700..7634
                     /gene="asnB"
                     /locus_tag="PKN55_07815"
     CDS             5700..7634
                     /gene="asnB"
                     /locus_tag="PKN55_07815"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HNX71188.1"
                     /translation="MCGLAGAVWSKPREIDALAVAGSMQDAIRHRGPDSRGHWMDSNV
                     GVLLAHVRLSIVELSPAGHQPMISACGRYVLVFNGEIYNHGELRQALCAAPIFRGHSD
                     TETLLACFAEWGIERTLQAAVGMFAFALWDRDTQTLTLGRDRLGEKPLYYGWQDDVLL
                     FASELNAICAWPGVSPELNRNALTLYMRHGFIPAPHSIWQGIVKLPPGCWVQFDAQSR
                     QQRLFPPVHAYWSLPAVWSAGKASPLAVSDEQAVDQLEQLLMQAVQGQMLADVPLGAF
                     LSGGVDSSTIVALMQASASRPVKTFSIGFDDPQYNEAGFAKAVAAHLGTEHHELYVSG
                     ADAMAAIERLPHCYDEPFADSSQIPSMLVAQLASQSVTVALSGDGGDELFAGYSRYAQ
                     TAYLRQRLQGLPRLARLVAQGGGALLQPALSWAGQYSSRASALSGQLDKLRILLAADD
                     QAFYQAQISYCQHPSRWVLDAQEPAYGLNTLSTLPQACSFSEKMTGWDMLNYLPDDIL
                     TKVDRACMGVGLESRVPLLDHRVVEFACHLPMQFKVRQAQRKWVLRQVLYRHVPQALI
                     ERPKKGFSAPVRQWLCGPLRDWVETLLDAQRLRQEGVFHVGNVRRAWRDCLAGQGSQH
                     HLLWHVVVFQAWRAHFLRSR"
     gene            complement(7873..>8053)
                     /locus_tag="PKN55_07820"
     CDS             complement(7873..>8053)
                     /locus_tag="PKN55_07820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX71189.1"
                     /translation="AKIYTTKASTPFFRKVCKVRNSAISAPPVVSAVINTRILIYFLY
                     PLFYAHANHDSAKGC"
BASE COUNT         1479 a         2009 c         2533 g         2032 t
ORIGIN      
        1 ttcgatgcat ggctgaaaaa tcattacaaa taatcacagg aatacgcaga tgacacgcaa
       61 gggcatcatt ctggccgggg gcagtggcac ccggctgtat ccggccacac tggcggtttc
      121 caagcaattg ctgccggtgt acgacaagcc gatgatctat tacccactca gcaccctgtt
      181 gctggcgggc atacaggaca ttctgatcat ttccaccccg caggataccc cgcgctttgc
      241 ccaattgctg ggcgatggca gccagtgggg gatttctctg tcttacgcgg tgcaacccag
      301 tccggatggc ttggcgcagg cgtttatcat tggtgaatcc ttcctgaatg gtgcgccgtc
      361 ttcgctggtg ctgggcgaca atatttttta cggccatgcc ctggttgagt tgcttggccg
      421 agcggcagcc aagcccagtg gagcttccgt gtttgcctat caggtcaacg accccgaacg
      481 ctatggtgtg gtggagtttg acgccgaagg ccgtgcgctg agcctggagg aaaaacccgc
      541 ccagcccaag tctcgctttg ccgtcaccgg cctgtatttc tatgacgagc aggtggtgga
      601 catcgccaag cagattcagc cttcgccgcg cggcgagctg gaaatcaccg acgtcaatcg
      661 cgtgtatctg gagcaaggcc agctggatgt ccaactgatg cgtcgtggct atgcctggct
      721 ggacaccggc acgcatgact ccatgctcga ggctgggcaa ttcattcaaa ccctggaaaa
      781 acgacagggt ttcaaggtgg cgtgcccaga agaaatcgta tggcgccagg ggtggattga
      841 tgatgcccaa ctggctcgcc tggctgcccc cttggccaag gtgggctatg gcttgtatct
      901 gaatcagttg ctggctgaat caaatccata accgtgcgcg gcctggcctt ctgattggaa
      961 ggcgcctggc tgaccttaac gtattttgca tgtgagtgca tgaggcgtgt atgagtagtc
     1021 ctatcccgtt tctggattta aaagcgatca atcagcgtca tcgtgacgcc ttgctgcaag
     1081 cttgtgaacg agtcatcgac tcgggctggt atgtgctggg cggggaggtc agcgcctttg
     1141 aagcggcgtt tgcgcaatat tgtgaagcgc ctcattgcgt cggcgtcgcc aatggcctgg
     1201 atgcgctgac actgattcta ttggctgatg gcattggccc gggcgacgaa gtgctggtgc
     1261 cgtccaacac ctatatcgcc acctggctgg cggtgagtgc cgtgggcgcc acgccggtgc
     1321 cggttgagcc ggatcccttg acttacaata ttgatccggt tgccgcggct gcggcgatca
     1381 cctcccggac caaggcgatt ctggccgtgc atttgtatgg ccagccggcg gatatgcagg
     1441 ccttggctgc attggctcag gcccatggct tgcgtttgta tgaagatgcg gcgcaagggc
     1501 atggcgcgcg ctgtcacgga cgccgtgccg ggtcgctgtc ggatgctgca ggcttcagct
     1561 tttatccggg caagaatctg ggtgccctgg gcgacggcgg cgccgtggtc actcgagacg
     1621 ccgcattggc cgatcgcgtt cgtgtgctgg ccaattatgg ctcgcacatc aaatatcaca
     1681 atctggtgcg tggggtgaat tcccgtctgg ataccttgca ggcggcgctg ttaatgacca
     1741 agctgccgtc cctggatgcc gataacgccc atcgccacac ggtggcccaa cgttatctgg
     1801 atggcctggc ggcgtgcccc ttgcaattgc cgcacacacc ggcctgggcg gagtcggtgt
     1861 ggcatgtgtt tgtggtgagc gcgccgcagc gcgatgcgct ggcggcggcg ctcaaggcgg
     1921 cggacatcgg ttcgctgatt cattacccga ttccgccgca tttgcagcct gcttatgccg
     1981 atctgggcat cccggctggc gcgctgccga ttgctgagcg catggccgag caggtgctca
     2041 gtttgcccat gggccccacc ttgtcgctgc aagaggttga ccgggtgatt gcggcgatcc
     2101 ggcagcactt tggtgtgggc gcttgatggc gcgaccgcgt ttagtggcgc tgattgccgg
     2161 gaccagcggc gccaagctgt acagcatggg attgaatatt ctggcgctgg cgttgaccgc
     2221 ccgctggttg gggccggatg gccgcggcat gttggccacc ttgaccacct gggtgaacac
     2281 cgccgccatg ttggccagct tgagtcttgg ctcggtggcg atgcagcgct tggccggttg
     2341 tcgtgacagc gcccgctttg gcgacgtgtt tcacgctttg ttgaggtttg cctggatact
     2401 ggccatttgc gcctggggcg tcatggcctt gttgtggctg gcgcgcggca gcgcattgtt
     2461 tggcaatgcc ccagcatggg ctttggcctt ggcgtgttta atgttgcctt gcttggtgtg
     2521 ggaaggctac gccaatgcct tgctgaccac catcgagcgc ttgcgcgtgt ttaatctgtc
     2581 gctggtgacg ggacgcagcc tgatgctgct gggtttgttg tgcctggccg tattggatgg
     2641 cgtcaccctg gccggcgtat tggcggtgtc tgtgttggcg cagtgctgga ttgccggcta
     2701 tagcttcccg acattgcgcg catgggcccg acagcatcaa gcggcagggg ccagcacggc
     2761 agaaggccgc gccttattgc atggcggcat gcgcttgcat ctgaacaccg ttggcacgct
     2821 ggccttgaat atggcaggca cactcttgat cgccaatatg ctgggcgccc gtgaagcggg
     2881 gttttttcag ctggcgcagc aattgatgac cgtgctgttg gtgattccgc agtctgcttc
     2941 tttggtgatt tatggccgcc agggcgaact ggggcctgat ggcgtgtggc cgttgcaacg
     3001 taaaatgctg ttgggtgtgg tggcgctgac tgccatcgcg ggattggcca tggcgatgag
     3061 cgcgcatatt tggctgcctt gggtggcggg tcctggctat gagccagcca ttcccttgtt
     3121 attggccatg ctgcctatcc tggtcatgct gagtttttct tccatgatgg cgtcgcagtg
     3181 gatcggccgt gggttttttc ttactgcatc actgcttacg gtgctggtgg gcttggtgaa
     3241 tttactggcg tcctcggtgt ggattccgga gtttggcatg atgggtgcgg tctatgcaca
     3301 gcttttggcg ttgctggtcg cggtgttcac caatagcagc atggcattgt ggtgtgaact
     3361 gaaaagccgc cgtctacggg cagaaagtgg aaaatgaaag tattgttgtt gggagaattc
     3421 agcgccctgc atcgagattt gtgcgatggt ttgctggcct tgggccatga tgcgcgtgtg
     3481 gcgtccaccg gggatggctg gaaagccatt ccgacagaca tcatgttagg ttcgaatgcg
     3541 tcaggcctga agggccgcct ggccagatta tggagtccgc tggcttctct gcccaagctg
     3601 atcaatcatg atgtgattca atttatcaat ccggtcttgt ttagcaccca atttgggtat
     3661 aacgaaaaac tgatccgcat gttgtttgcc cagaacaaca agtcgttcct ggtcgcagcc
     3721 ggatgtgatg ctttttactg gaatgaagga cgtcagcgtt tgccatacgg cccctttgcc
     3781 gattttgagc agcaggacct aggagggaat tccagcccat ggcgttcacc cgtgcggcga
     3841 aaattcaacc agtggctggc tgaaaatgtc gatggcatca ttccgatcat gtacgactat
     3901 tgggtaggct atcaaggctt tcccaatcta tgttcgccat tgccgattcc ggtaaacctg
     3961 gaaaaaatta aatatcagga aaacaccctg aagcaaggca agctggtggt gtttcatggc
     4021 ttgaatcgtg aggggttcaa gggcacgggc tatgtaagaa aagcttttga catattgtct
     4081 aataagtatt ctggtgaagt ggagtgcgta attcagggcc ggatgccgct caaggactac
     4141 atgaagcttt tgtcgacggt gaatgtggtg gtggaccaga cctcttcaca tggcagtggg
     4201 gtcaatggct tgttttccct ggctttgggc aaggtggtgt tgggcggcaa cgagccggcg
     4261 catcaggcgc tgatgtatgg cgacgaccct tgtcccattc gcaatatttc tcaagacccc
     4321 atgtcgattg tggctcaggt tgagcagatt atggcgcaac gtgatcaatt ggccgccatg
     4381 gggcaggctg gccgtgaatt catcatgcgc caccatgatc atcggcgcgt cgctgctcgc
     4441 tatgtcgata cttggcaaat ggagagtaaa aaatgagcgc gtcactggac gtgctgtttc
     4501 ttatccgttc tttgcacata ggtggcgctg agcgacaact ggctttgctg gccagaacca
     4561 tgcatgcttc tggccatcgg gtgcgcgtcg cgacatttta tgcctctgga gcatttgaag
     4621 ctgatctgcg acaggccggc gtgccggtgc ttgacttgaa aaaatctggt cgctgggatg
     4681 tgttaacgtt tttctggcga ctggtccggc tgctgcgcaa agaaaaaccc gtgattgtgc
     4741 acggttatct gccaatgtcc aatctgctgg ccttgctggc caagcccttt gctggttggc
     4801 cgaaagtggt gtggggcatt cgttcctccg gctctgatcc tcggcattat ggctggatgg
     4861 gcaggctgga ggcctggttg caggcgcgct tgtcccgttg ggccgattta atcatttgta
     4921 attctcagcg tggtcggcaa tacgccttgt cgcagggatt tcccctggcc cgcactgtat
     4981 gcgttccaaa tggtgtgaac tgtgagcagt ttttccccga tccagcggct gggctgcgca
     5041 cgcgagaagc ttggggcatc tcgcaagatg tgtatctgat cgggattgtt gggcgctttg
     5101 tgcccttgaa aaaccatgcc ggtttgctgc gcgccgccgc tcctttgctg ttgactcagc
     5161 cttgtgccgt ggtgtgcatt ggttctggct cgtcgcaagc tatccaggag atgcgcgcct
     5221 tggcggtgga gttgggcatt gcggacaaag tattttttcc gggcgcccat ttggaaatgt
     5281 gcgctgcgta caatgcgctg gatttattcg tttctgcttc gctgactgaa ggtgcgccga
     5341 atgtgctggg tgaggccatg gcctgtaatc ttccggtgat ctgcaccgat gtgggtgatg
     5401 ctgcgtggtt ggcggcggat tatggccaag tggtttcgcc aggggatgtg cctgccttgg
     5461 agcgcgctat tttgcacgcg tatgcctgcc gtaaaatccc acgggcctca cccagggaac
     5521 gggtgttatc agcattcagc gtcatggctt tggccgccaa tacagagacg ctattgcgca
     5581 atgtgtgtac tgcctctgaa acaaaaaccc catgaagatg ccgtatgtgc gggctgaggg
     5641 gcatcaatgc tgagcatctg gaatgctcat acgccgcagg atggcgaaaa ggttgagtta
     5701 tgtgtggatt agctggagca gtatggtcaa aaccacgtga gatcgacgcg ctggccgtgg
     5761 cgggcagcat gcaggacgca atccggcatc gtgggccaga cagtcgcggt cattggatgg
     5821 acagtaatgt tggcgtgctg ctggcgcatg tgcgcttgtc gattgtcgag ctgtcgccag
     5881 caggccatca gccgatgatt tcggcttgtg ggcgttatgt gttggtgttc aatggcgaaa
     5941 tctacaatca tggcgaatta cggcaagctt tgtgtgcagc ccccatattt cgaggtcatt
     6001 ccgacacaga aaccctattg gcgtgttttg ccgaatgggg cattgagcgc accttgcaag
     6061 ctgccgtggg tatgtttgcc tttgccttat gggatcgaga cacgcaaacc ctgacacttg
     6121 ggcgtgatcg tttgggtgaa aaacccttgt attacggctg gcaggacgat gttttgttat
     6181 ttgcctccga acttaacgcc atctgtgcat ggccgggtgt ttcacctgaa ttgaaccgca
     6241 atgcgcttac cctatatatg cgccatggtt ttatccctgc tccgcattct atttggcaag
     6301 gcattgtcaa gctgccgccg ggctgctggg tgcaatttga tgcgcaaagt cgccagcaaa
     6361 ggctgtttcc acctgttcat gcttattggt ctttgcctgc tgtgtggtct gccggcaagg
     6421 cgtcgccatt ggccgtctcg gatgaacagg ccgtggacca gttagagcaa ttgttgatgc
     6481 aagcagtgca aggtcagatg ctggcggatg tgccattggg ggcttttttg tctggggggg
     6541 tggattcctc gacgatcgtg gcgctgatgc aagcctctgc atcgcgcccg gtcaaaacct
     6601 tttcgattgg ttttgacgat ccgcagtata acgaggctgg ttttgccaaa gctgttgcgg
     6661 cgcacttggg gactgaacat catgaactgt atgtcagcgg cgccgacgcc atggccgcca
     6721 ttgagcgtct gccgcactgc tatgacgaac catttgccga cagttcacaa attccctcga
     6781 tgctggtggc gcaattagcc agccagtcag tgaccgtggc tttgtctggc gacggcggcg
     6841 atgaattgtt tgctggctat agccgctacg cgcaaacggc ctatttgcgt cagcgcctgc
     6901 agggtttgcc gaggctggcc cgtctggttg cgcagggagg aggcgcacta ttgcagcctg
     6961 cattgtcttg ggctgggcaa tattcgtcgc gcgcttcggc gttgagtggg caactggata
     7021 aactgaggat cttgttggct gcggatgatc aagcatttta tcaggcgcaa atttcttact
     7081 gccagcatcc ttctcgctgg gtgctggatg ctcaagaacc agcatatgga ttgaatacgc
     7141 tcagcacatt gccacaggcc tgttcatttt ccgaaaaaat gactggctgg gacatgctga
     7201 actatttgcc agacgatatt ctgaccaagg tggatcgcgc ttgtatgggc gttggcttgg
     7261 aaagccgcgt gcctttgctg gatcatcggg tggtggagtt tgcttgccat ttacccatgc
     7321 aattcaaagt gcggcaagca cagcgtaagt gggtgttgcg tcaggtgctt taccgtcatg
     7381 tgccacaggc cttgattgag cgcccgaaaa aagggttttc agcgccggtc cgacagtggc
     7441 tgtgtgggcc gttgagggat tgggtggaaa cgttgctgga tgcgcaacgt ttgcggcaag
     7501 agggggtgtt ccatgttggc aatgtgcgcc gagcatggcg ggactgtctg gccggtcaag
     7561 gcagccaaca ccatttactt tggcatgttg tggtgtttca ggcgtggcga gcacattttt
     7621 tacggtcaag atgatggtgt ggcgcatgtg ggctgatgtg caaagaatgg ttgtggcgat
     7681 gccggcggcc gtgcgctgag tcagtgcagc acagggcgtt gtcggccata ctgggggcgg
     7741 ctgcaaaata tgctttttca tgccagcaag ctcggctaag gaacgctgca aaaatcatca
     7801 tttcagcgct gggttttgat gttaatgggt tcgtttaggg gatggggttg taatcaataa
     7861 atcagcgcac ccttagcagc ccttggcgga atcatgattg gcatgagcat aaaatagtgg
     7921 gtagagaaaa tatatgagaa tcctggtatt gattactgca ctgacgacag ggggggctga
     7981 aatggcgctg tttcgcacct tgcacacctt gcgaaaaaaa ggcgtcgatg ctttggtggt
     8041 atagattttg gca
//
LOCUS       DAOPYX010000129         8019 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_10777_length_8019_cov_5.292818, whole genome shotgun sequence.
ACCESSION   DAOPYX010000129 DAOPYX010000000
VERSION     DAOPYX010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 8019)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8019)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8019
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10777_length_8019_cov_5.292818"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            459..770
                     /locus_tag="PKN55_07825"
     CDS             459..770
                     /locus_tag="PKN55_07825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011135928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX71190.1"
                     /translation="MQHKPILILWPSFLIALVMTTAFFSVFDPMELTLHGQALFISPL
                     AGYTAFFFASWALCALVSALTLYLQHSSAEINGFAPPAIVDDGNPDDPDARLRASLDG
                     R"
     gene            879..3125
                     /locus_tag="PKN55_07830"
     CDS             879..3125
                     /locus_tag="PKN55_07830"
                     /inference="COORDINATES: protein motif:HMM:TIGR01596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas3''"
                     /protein_id="HNX71191.1"
                     /translation="MLTPIAHTPADGCSPPHLLDEHLRKVGELAASFAAPAWADWARQ
                     AGRWHDLGKYDPAFQDYIRRDSGYEAHLLDGQPSHPSHSLAGAQHALLTQPKFGRLLA
                     YLIAGHHTGLPDGPAEYQGAASLSHRLAQGPQALADALRADIPADLLAATPLTLNPQA
                     IGGKDGLHLWLRLVFSCLVDADFLDSEAYFQPEQAQQRRPPPDLADLRARLDAYLADK
                     HAHAAPSAVNSVRADVLRQCREQAQQPPGCYTLTVPTGGGKTLSSLAFALEHALAHGK
                     RRVIYAIPYTSIIEQTAEVFRAALGDDAVLEHHSSLDVEHETVHSRLAAENWDASVIV
                     TTNVQLFESLFAARTSRCRKLHRLVDSVIVLDEAQLLPIPYLQPLLDALRLLTEHYGV
                     TLLLCTATQPALHTRSNSFGDIQLRGLDARRAIIQREAQLYQQLARVRLHLPADLDAR
                     QSWADIARQISQHPSVLVIVNTRRHCRELHALLPDGTFHLSALMCAEHRSQQIADIRQ
                     RLARGEPVRVVSTTLIEAGVDVDFPVVFRALAGLDSIAQAAGRCNREGRLPHGDVHVF
                     VPPDPSPLGWQRAAEEAARTVLGQCGADALAPDAMQRYFNHYYGKAGQAGFDREGLHA
                     LLTQHAGQGEIFFRTAAERMRLIDNAHTVAVIVPYTRQDGECSRDSHALRCRLQAGEM
                     HRDLLRQLQRFTVSLPQPQFERLRRAGELEEIYRDIWWVKNDVMYDEKRGLLVGEIEA
                     ANPEQLLC"
     gene            3231..3905
                     /gene="cas5c"
                     /locus_tag="PKN55_07835"
     CDS             3231..3905
                     /gene="cas5c"
                     /locus_tag="PKN55_07835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009117237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas5c"
                     /protein_id="HNX71192.1"
                     /translation="MQPFVLHVTGDFACFTRPEMKVERVSYDVITPSAARAIFESILW
                     KPAIRWQIRRIDVLAPIRWTTLRRNELAGKLSLASVHSAMRHGVGQLAQYIEEERQQR
                     ASLLLRDVAYRLYADFSLTERAGPDDSLVKFAEMFRRRASRGQCVNQPYLGCREFAAD
                     VSLASPDQAAPPCPPTLRGEYPLGWMLYDLDFTQADDPRPRFYRPVMRDGVIHVPDWQ
                     SEEVLG"
     gene            3902..5677
                     /gene="cas8c"
                     /locus_tag="PKN55_07840"
     CDS             3902..5677
                     /gene="cas8c"
                     /locus_tag="PKN55_07840"
                     /inference="COORDINATES: protein motif:HMM:TIGR01863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas8c/Csd1"
                     /protein_id="HNX71193.1"
                     /translation="MILQALTDYYHRKAEAAGPGQAALAPAGFEHKEIPFVLELDHAG
                     QLVNLINTQQPVGKKLRARSYQVPQGVKKTSGVAANLLWDTVEYVLGIDTKGKPERVA
                     QQHAAFVARLDELPADDAGVRAVRAFLADIPWDTLHAHPDWETLLTVNPVITFQLQDD
                     FGELVCTRPAIVAHLQGGPASADNSAPGICLVSGETQPIARLHPAIKGVWGAQTSGAN
                     IVSFNQRAFESYGKEGRQGENAPVGEATVFAYTTALNHLLGKDSRQRLQVGDASTVFW
                     AAQPCAMENSLADLFNEHKDNPDAHSQAVSALYRSVQHGQFVDEGGDTRFYVLGLAPN
                     AARLSVRFWHCATVAELATRLVRHFDDLNIIRGPFDPPHLSLIRLLGCTALQGELKHL
                     PPSLGGDTLRAILDDRPYPDTLLAACIRRIRAEQKVTYPRAALLKAWLNRWARAYPNA
                     IHPQEDITVSLNPDRTDPGYCLGRLFAVLERLQERASSGRKLNATICDRFYGSASSNP
                     VMVFPNLLKLAVHHHSKIAQHAPGEAVNLSKLLGSVMSKLDLFPTQLTLQDQALFALG
                     YYHQKHDFFQKPDTDTAADAQGDAQ"
     gene            5674..6651
                     /gene="cas7c"
                     /locus_tag="PKN55_07845"
     CDS             5674..6651
                     /gene="cas7c"
                     /locus_tag="PKN55_07845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003777774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas7/Csd2"
                     /protein_id="HNX71194.1"
                     /translation="MTALAHRYDFVLLFDVTDGNPNGDPDAGNLPRIDAETGLGLVTD
                     VCLKRKVRNYISLAHDQSPREPQAGEKRYEIYVREKAVLNLQHQRAYAALNLEAAEAP
                     AEAPTEDASEEKPSKGKARAPAKEKRKGSAGDAEQARAWMCQNFYDVRTFGAVMSTGV
                     NCGQVRGPVQLTLARSIDPIVPQEHSITRVAVTTEAEAEKQQGDNRTMGRKYTVPYGL
                     YRAHGFVSAHLARQTGFDETDLQQLWQALSQMFEHDHAASRGQMSARALYVFRHDSEL
                     GNAPAHRLFDLITIRRREDVAVARHFSDYQITINREHIPAGVQLDEQAF"
     gene            7039..7914
                     /locus_tag="PKN55_07850"
     CDS             7039..7914
                     /locus_tag="PKN55_07850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX71195.1"
                     /translation="MIQLEQQQVAGLAPGEDIDGQEAYEAWCRVKGLAESGATPQIDA
                     RDVLGALVFCGRENKALLEKKTSAGRAYQNHIAWLEDQLAKARASTGGSRELVAASIS
                     IRDRLVDYFRYVAEGLPLERQAEANERAQALINAIGLLANDLEAGRLAGQCIRLHRNQ
                     LLGELDEARAALDASQGQAKQLEAEVAELHDLADRQAGLLSRTAIAIRGPEPDLTRWS
                     HHDLPELAAAMRKQRDICASAANKIQWATPDYAHQEGACPECGQYEHDGHGEGCMVGR
                     ACGIYDVLRLTGALK"
BASE COUNT         1571 a         2765 c         2362 g         1321 t
ORIGIN      
        1 aatcgtacga caaggtcaca caacaacgcc tggggggctc taagcgcggc tcacaaacgc
       61 ctcctggcgc gcttgccaga cagccgtact gtgtacggcc gcccgccccg gcaggatggg
      121 ccgatacaac acgcagcaaa gcgctgctgg ccaagcatgt ggccgcaaca gcaccgccag
      181 gcaaaagcat gttatgcgca gcgctacggc gagagccgcg cgccaagccc atccccccgt
      241 ggttttggcg agcgtttctc cgcgtttctc aatggtgttg atgagtttgg gcgacggcta
      301 cccccgtcgc catttttttg gcatctcgct ccaaatccac tacgctgaag cgaagggcgg
      361 ccgcacgccg ccgcctgatt tcccatcatc accctgcccg cccatgcggc aagacgggca
      421 gggggcaagc ctggttgccg cgttgaagga gtcatgtcat gcagcacaaa cccattctga
      481 ttctgtggcc ttcttttctg attgcgctgg tgatgaccac cgcgttcttc tcggtattcg
      541 accccatgga actcaccctg cacggccagg cgctgttcat cagcccgctg gccggttata
      601 ccgccttctt ttttgcctcc tgggccttgt gtgcgctggt cagcgcgctg acgctgtact
      661 tgcagcacag cagcgccgaa atcaacggct ttgccccacc cgccatcgtc gatgacggca
      721 accccgacga ccccgacgcc cgcctgcgcg cctcgctgga tgggcgttaa gagcggctaa
      781 cagcaccgcc ctgcaccgcc aaaaggacgt tgcaagcacc gccaagccat catcatgctg
      841 ataggatgga gtcctgactt cacacgcagg actccaccat gctcaccccg attgcccaca
      901 cccccgcaga cggctgctct ccaccccacc ttttagatga acacctacgc aaagtgggcg
      961 aactggcggc aagctttgcc gccccagcct gggccgactg ggcgcgccaa gccgggcgct
     1021 ggcacgatct gggcaaatat gacccggcgt ttcaagacta tatccgccgg gatagcggct
     1081 acgaagccca tctgctggat ggccagccca gccaccccag ccactctctg gctggcgcac
     1141 agcatgccct tcttacgcag ccgaagtttg gccgcctgct ggcctacctg attgccggcc
     1201 accacaccgg cctgcctgac ggcccagccg aatatcaagg cgccgcctcg ctcagccatc
     1261 ggctggcgca aggcccgcaa gcgctggccg acgccttgcg cgccgacatt cccgccgacc
     1321 tgctggccgc cacgccgctg acgctgaacc cccaagccat cggcggcaaa gacggcctgc
     1381 acctatggct gcgtttggtg ttttcttgcc tggtggatgc tgattttctc gacagcgaag
     1441 cctattttca gcccgagcaa gcccagcagc gccgcccgcc gcccgatctg gctgacttgc
     1501 gcgcccggct ggatgcctat ctggccgaca aacacgccca tgctgcgccc agcgccgtca
     1561 acagcgtgcg cgccgacgtg ttgcgccagt gtcgcgagca ggcgcagcag ccacccggct
     1621 gctacaccct caccgtgccc acgggcggcg gcaaaaccct gtccagcctg gcctttgccc
     1681 tggagcacgc attggcgcac ggcaaacgcc gggtgattta cgccatcccc tacaccagca
     1741 tcatcgaaca aaccgctgaa gtgtttcgcg cggccctggg tgacgacgcc gtgctggagc
     1801 accacagcag cctggatgtc gaacacgaaa ccgtacacag ccggctggcc gccgaaaact
     1861 gggacgccag cgtgattgtc accaccaatg tgcagctgtt tgaatcgctg tttgccgccc
     1921 gcaccagccg ctgccgcaaa ctgcatcgcc tggtggacag cgtgattgtg ctggacgaag
     1981 cccaactgct gcccatcccc tatctgcaac ccctgctcga cgccctgcgc ctgctgaccg
     2041 agcattacgg cgtcaccctg ctgctgtgca ccgccaccca gccagcgctg cacacccgca
     2101 gcaacagctt tggcgatatc caattgcgcg ggctggacgc ccgtcgcgcc atcattcagc
     2161 gcgaagccca gctgtatcaa caactggcgc gtgtgcgcct gcacctgccc gccgacctgg
     2221 acgcccggca aagctgggcc gatatcgcca gacaaatcag ccaacacccc agcgtgctgg
     2281 tgatcgtcaa cacccggcga cactgccgcg aactgcacgc cctgttgccc gatggcacgt
     2341 ttcatctatc ggcgctgatg tgcgccgaac accgcagcca gcaaatcgcc gacatccgcc
     2401 agcgcctggc cagaggcgag ccggtgcgcg tggtgtccac caccttgatc gaggccgggg
     2461 tggatgtgga ttttccggtg gtgtttcgtg cgctggccgg gctggactcc atcgcccaag
     2521 cggctggccg ctgcaaccgc gaaggccggc tgccgcatgg cgatgtgcat gtgtttgtgc
     2581 cgcccgaccc atccccactg ggctggcaac gcgccgccga agaagccgcc cgcaccgtgc
     2641 tgggccaatg tggcgccgac gccctggccc ccgatgccat gcaacgctac ttcaatcact
     2701 actacggcaa ggccggccaa gctggttttg accgcgaggg cctgcacgcc ctgctgaccc
     2761 aacacgccgg ccagggcgag atattttttc gcaccgctgc cgagcgcatg cgcctgatcg
     2821 acaacgccca cacagttgcc gtcatcgtgc cctacacccg acaagacggt gaatgcagcc
     2881 gcgacagcca cgccttgcgc tgccgcttgc aggccggcga aatgcaccgc gacctgctgc
     2941 gccagctgca acgctttacc gtcagcctac cccagccaca gtttgagcgg ctgcgccggg
     3001 cgggcgagct ggaggaaatc tatcgcgata tctggtgggt gaaaaacgac gtgatgtatg
     3061 acgagaagcg ggggctgttg gtgggggaga ttgaggcagc caacccggag caactgcttt
     3121 gttgagggtc aaataccgcc catcgcaaac agattatgct tttgtatttg cccgccaccc
     3181 agcacaggct atctggggcg ccatctgcgc ctgaactcag gagggacgtg atgcaaccct
     3241 ttgttttaca tgtcacaggc gactttgcct gctttacccg cccggaaatg aaagtggagc
     3301 gcgtcagcta cgacgtcatt accccatcgg cggcgcgggc aatattcgag tcgattttat
     3361 ggaaacccgc catccgctgg cagatacgcc gcattgatgt gctggcgccc atccgctgga
     3421 ccaccctgcg gcgcaatgag ctggccggca agctgtcgct ggcctcggtg cacagcgcca
     3481 tgcgccacgg tgtgggccaa ctggcgcagt acatcgaaga agagcgccag cagcgcgcca
     3541 gcctgctgct gcgtgacgta gcctatcgat tgtatgccga tttcagcctg accgaacgcg
     3601 ccggcccgga tgattctttg gtgaaatttg ccgagatgtt tcgccgccgc gccagccgtg
     3661 gccagtgcgt caatcagccc tatctgggct gccgcgaatt tgccgccgac gttagcctgg
     3721 cctcgccaga ccaggccgcg ccaccctgcc cgccaacgct gcgcggcgaa tacccgctgg
     3781 gctggatgct ctacgacctg gactttaccc aggccgacga cccgcgcccg cgcttttacc
     3841 ggccggtgat gcgcgatggc gtgattcatg tgcccgactg gcaaagcgaa gaggtgctcg
     3901 gatgattctg caagccctga ctgactatta ccaccgcaag gcagaagccg ccggccccgg
     3961 ccaggcggcg ctggccccag caggctttga acacaaagaa attccgtttg tgctggagtt
     4021 ggaccacgcc gggcaactgg tgaatctgat caacacccag caaccggtgg gcaaaaagct
     4081 gcgcgcgcgc agctaccaag tgccgcaagg cgtgaaaaaa acctccggcg tggccgccaa
     4141 tctgctgtgg gacaccgtgg aatacgtgct gggcatcgac accaaaggca agcccgagcg
     4201 ggtggcgcag cagcacgcgg cgtttgtcgc ccggctggac gagctgcccg ccgatgatgc
     4261 cggggtgcgc gccgtgcgcg cttttctggc ggatatcccc tgggacacgc tgcacgccca
     4321 cccagactgg gaaaccctgc tcaccgtcaa cccggtgatc acttttcagc tgcaagacga
     4381 ctttggcgaa ctggtgtgca cccgcccggc cattgtggcc cacttgcaag gcggcccggc
     4441 cagcgccgac aacagcgcgc caggcatttg cctggtgagt ggcgaaaccc agccgattgc
     4501 ccgcctgcat cccgccatca aaggcgtgtg gggcgcgcaa acctcggggg ccaatattgt
     4561 ctcgttcaac cagcgcgcgt ttgaatcgta tggcaaagaa ggccgccagg gcgaaaacgc
     4621 cccagtgggc gaggccaccg tctttgccta caccactgcg ctcaaccatt tattaggcaa
     4681 ggactctcgc cagcgcctgc aagtgggcga cgcctccacc gtgttctggg cggcgcaacc
     4741 ctgcgccatg gaaaacagcc tggccgacct gttcaacgaa cacaaagaca acccggacgc
     4801 ccacagccag gcggtcagcg cgctgtatcg ctcggtgcag catggccaat ttgtggatga
     4861 aggcggcgac acccggtttt atgtgctggg cctggccccc aacgccgcgc ggctgtcggt
     4921 gcgcttttgg cactgcgcca ccgtggccga gctggccacc cggctggtgc gccactttga
     4981 cgacctgaac atcatccgtg gcccatttga cccgccgcac ctgtcgctga tccgcctgct
     5041 gggctgcacg gcgctgcaag gcgagctgaa acacctgccg cccagcctgg gcggcgacac
     5101 cctgcgcgcc atcctggatg accgccccta cccggacacc ctgctggccg cctgcattcg
     5161 ccgcatccgc gccgaacaaa aagtcaccta tccccgcgcc gccctgctga aagcctggct
     5221 gaaccgctgg gcgcgcgcct atccaaacgc cattcaccca caggaggaca tcaccgtgtc
     5281 gctcaaccct gaccgcaccg accccggcta ctgcctgggc cgtttgtttg ccgtgctgga
     5341 gcgcctgcaa gaacgcgcca gcagcgggcg caaactcaac gccaccatat gcgaccgctt
     5401 ttatggctcg gcttccagca acccggtgat ggtgtttccc aatctgctca aactggccgt
     5461 gcatcaccac agcaaaattg cccagcacgc tcccggcgaa gccgtcaacc tgagcaaatt
     5521 gctgggcagc gtgatgagca agctggatct gtttcccacc caactgaccc tgcaagacca
     5581 agcactgttc gccttgggct actaccacca aaaacacgac ttttttcaga aacctgacac
     5641 cgacaccgcc gctgacgccc aaggagacgc ccaatgaccg ccctcgccca ccgctatgat
     5701 tttgtgctgc tgtttgacgt gaccgacggc aaccccaacg gcgaccccga cgccggcaac
     5761 ctgccgcgca tcgacgccga aaccggcctg ggcctggtga ccgacgtttg cctgaagcgc
     5821 aaagtgcgca actacatcag cctggcccac gaccaaagcc cgcgcgagcc gcaagccggc
     5881 gaaaaacgct atgaaattta cgtgcgcgaa aaagccgtgc tcaacctgca acaccagcgc
     5941 gcctacgccg cgctgaacct ggaagccgcc gaagcgcccg ccgaggcccc gaccgaagac
     6001 gccagcgaag aaaagcccag caaaggcaaa gcgcgcgcgc ccgccaaaga aaagcgcaaa
     6061 ggcagcgcgg gcgacgccga gcaagcccgc gcctggatgt gccagaattt ttacgatgtg
     6121 cgcacctttg gcgccgtcat gtccactggc gtcaactgcg gacaagtgcg cggcccggtg
     6181 caactcaccc tggcccgctc aatcgacccg attgtgccgc aagaacacag catcacccgt
     6241 gtggcggtca ccaccgaagc cgaagccgaa aaacaacaag gcgacaaccg caccatgggc
     6301 cgcaaataca ccgtgcccta tggcctgtat cgcgcccacg gctttgtctc cgcccacctg
     6361 gcccggcaaa ccggctttga cgaaaccgac ctgcaacaac tgtggcaggc gctcagccag
     6421 atgtttgagc acgaccacgc cgccagccga ggccaaatga gcgcccgcgc cctgtatgtg
     6481 ttccgccacg acagcgaact gggcaacgcc cctgcccatc ggctatttga cctgatcacc
     6541 atccgccgac gcgaagacgt ggccgtggcg cgccacttca gcgactatca aatcaccatc
     6601 aaccgcgagc acatccctgc cggcgtccag ctggacgagc aggcattcta agcccacatt
     6661 gaccgcgcca cccccggcgc ggacaatgcc cacacccgcc ggccccagcc caagcgccca
     6721 ggatcgcccc gcgcgaaccc caagcgaacg gccaaacccg gcggggttcg cgctggagtt
     6781 agtccattga ataaaaagca cttttttctg gatgcatgga aatatgcctg cccgccatcc
     6841 aatgtgggcg gcaggttcgc gcgcgacgca gaaaaatggc tggaaattca aggggtttct
     6901 ggcgcgaagt cacccggtgg gtcaccaccg ggtgtggatt gaaacaaagg cgcgctcgat
     6961 tgtcagtttg gcgtgcacaa tgacctgctg gcccgcatcg ctgacatgga ggtcaaactg
     7021 gccgccagca atggcggggt gattcagctg gaacagcagc aggtcgctgg cctggctcct
     7081 ggcgaagata tcgacggcca agaggcttac gaggcatggt gccgcgtcaa gggcctggcg
     7141 gaatccggag ccacgccgca gattgatgcc cgcgacgtgc tgggcgcgct ggtgttttgc
     7201 gggcgcgaaa acaaggcgct gctggaaaaa aagaccagcg ctgggcgcgc ataccaaaat
     7261 cacatcgcct ggctggagga tcagctggcc aaggctcgcg ccagcaccgg cggcagccgc
     7321 gagctggtgg ccgcctcaat cagtatccgg gatcgcctgg tggactattt ccggtatgtc
     7381 gcggaggggt tgccgctgga gcggcaggcg gaggcaaacg agcgcgctca ggcgcttatc
     7441 aacgccattg gattgctggc aaatgacctg gaagccggcc ggctggctgg ccagtgcatc
     7501 agactgcacc gcaatcagct gctgggcgag ctggacgaag cccgcgcggc cctggacgcc
     7561 agccaggggc aggcgaagca gctggaggcc gaagtggcag agttgcacga tttggcagac
     7621 cgccaggccg gcctactcag tcggacggct atcgccatcc gtgggccgga acccgacctg
     7681 acgcgctgga gccatcacga tctgcctgag ctggccgctg ccatgcgcaa gcagcgcgac
     7741 atttgcgcca gcgccgcaaa caaaattcag tgggcgacgc ccgactatgc tcaccaggag
     7801 ggcgcctgcc cagaatgcgg gcaatacgag cacgatggcc atggcgaggg ctgtatggtt
     7861 ggccgggcat gtgggattta tgacgtgcta cggttgactg gggcgctgaa atgacccagg
     7921 tcaaagttaa agagctgcgc aacgccgcta tccgccgggg gtttgacgtg ctcaccggcc
     7981 cgagcatcgg gctttccgcg cgcagggcct gccttcggc
//
LOCUS       DAOPYX010000130         7954 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_10907_length_7954_cov_8.822636, whole genome shotgun sequence.
ACCESSION   DAOPYX010000130 DAOPYX010000000
VERSION     DAOPYX010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 7954)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7954)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7954
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10907_length_7954_cov_8.822636"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..845)
                     /locus_tag="PKN55_07855"
     CDS             complement(<1..845)
                     /locus_tag="PKN55_07855"
                     /inference="COORDINATES: protein motif:HMM:NF014474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB small GTPase domain-containing protein"
                     /protein_id="HNX71196.1"
                     /translation="MTASCPKCPPSGAGLSAIEAARQGRRLAVVGLPNAGKSTFFNRL
                     TGLSQRVGNWPGLTIDLASAKVVLGARAVEVVDLPGIHDLSGYSEDEAVVREVLAGSV
                     FDGLVVVVNGAQLDRHWRLVWQLAQLDLPLVVLVNMQDEMRQLGIRADLDALSARLGA
                     PVLAMSARKGENWPAVRAALTQLAAQIPAREAPVAIAALDALPAGLEEARAMLAGIHH
                     VPATLPATWTTRIDQLLLHPWLGLPMFTVLMALIFQATYLLGTPMQEGLGEGLDWVQA
                     QWWQPA"
     gene            complement(848..1081)
                     /locus_tag="PKN55_07860"
     CDS             complement(848..1081)
                     /locus_tag="PKN55_07860"
                     /inference="COORDINATES: protein motif:HMM:NF015956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA domain-containing protein"
                     /protein_id="HNX71197.1"
                     /translation="MTSLATLAAHHRAQVVAIDLPDTERQRLSAFGILPGAQVQVFRR
                     AALGGPLHVAVGATQLLLRRLLAEGIRVELQGA"
     gene            1414..1980
                     /gene="dcd"
                     /locus_tag="PKN55_07865"
     CDS             1414..1980
                     /gene="dcd"
                     /locus_tag="PKN55_07865"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004632155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HNX71198.1"
                     /translation="MSIKSDKWIRRMATTDGMIEPFEPNQVREVDGRKIVSYGTSSYG
                     YDIRCANEFKIFTNINSTIVDPKNFDHNNFVDFTGDVCIIPPNSFALARTVEYFRIPR
                     NVLTVCLGKSTYARCGIIVNVTPFEPEWEGYVTLEFSNTTPLPAKIYAGEGCAQVLFF
                     ESDEVCETSYKDRGGKYQGQVGVTLPKT"
     gene            2479..3627
                     /locus_tag="PKN55_07870"
     CDS             2479..3627
                     /locus_tag="PKN55_07870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018150141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III PLP-dependent enzyme"
                     /protein_id="HNX71199.1"
                     /translation="MDRIAADALAAAPELSLDFARVKAQLAKGYEKPFLILDTQIVRE
                     KCRRFFAAMPRVRPHFAVKSNPDQRVLQVLKEEGVGFEIASIAELDQLLALGVPPAEI
                     FYSNPMKSRAYVEYAVAKGVEWFVIDSVEELQKVHRASPTAKTYLRIYTTNVGSDWPL
                     SGKFGAYESEIRDIIAEAARLGADLAGVTFHVGSQCLNPENWKVGLETTRAVFDQMLA
                     AGLKPRLVNIGGGYPVTHTKDIPTIAEIGAVVNAGLAAFGDEVQVIGEPGRYLVSDAG
                     YFVCRVVGTATREGKRWVYFDAGMFGGIIETTEGLKYNILTDRSGPEIIWTVAGPTCD
                     SLDICTRDQALPQDLVEDDFIYIKNAGAYTTAYASTFNGFPLPDVIIL"
     gene            complement(3697..4230)
                     /locus_tag="PKN55_07875"
     CDS             complement(3697..4230)
                     /locus_tag="PKN55_07875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018747181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNX71200.1"
                     /translation="MHTALPLSGPRVHLRHWRDEDLPAFAALNADPQVMAHFPAPLSR
                     SDSDALAARLRAGLAERGWGAWVLARRDDDALLGCVGLNPVAASFPFAPAVEMLWRLA
                     RPAWGQGLAHEGAQLALAAARELGMSRVVAFTALGNLRSRALMARLGMLEQGEFDHPA
                     LAADSPLRRHVWCEWRA"
     gene            4334..5077
                     /locus_tag="PKN55_07880"
     CDS             4334..5077
                     /locus_tag="PKN55_07880"
                     /inference="COORDINATES: protein motif:HMM:NF018738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein"
                     /protein_id="HNX71201.1"
                     /translation="MLKKLLASIGVGSAQVDTQIDNPELAPGQLLQGQVLVQGGQSAQ
                     DIEKIELVLMTQAEDESQDSERTVAWPLCRLPVCGPFTIGAGETRYFPFQMTLPLETP
                     VNALRTRDRRVPLVWIHTDLAIAAGVDSDDRDQLLVRPGMALQTLLDAFARLDWYVHS
                     SDVELGSARVGPISSTLGCYQEIELRPHGGSWRIEEIELTCLCDGHHTHVLIEVDHRL
                     GGDHFYSLVMGPDFARRDWVAILRDTLPL"
     gene            complement(5282..6529)
                     /gene="glyA"
                     /locus_tag="PKN55_07885"
     CDS             complement(5282..6529)
                     /gene="glyA"
                     /locus_tag="PKN55_07885"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011134840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HNX71202.1"
                     /translation="MFAADQTIHAYDPELAAMMDAEFRRQEDHVELIASENYVSPAVM
                     EAQGSVLTNKYAEGYPGKRYYGGCEYVDQVEQLAIDRLKALFGAEYANVQPHSGSQAN
                     QAVYVSVLKPGDTILGMSLAHGGHLTHGASVNISGKLYNVVAYGLNEAEAIDYDEVAR
                     LAREHKPKMIVAGASAYALEIDWARFRAIADEVGAYLFVDMAHYAGLVAGGEYPNPVP
                     FADFVTTTTHKTLRGPRGGVILARAEHEKILNSAIFPCLQGGPLMHVIAAKAVAFKEA
                     ASDDFKVYAKQVKVNASAMAEELIKRGLRIVSGRTESHVFLLDLRAKQITGKDAEAAL
                     GRAHITVNKNAIPNDPEKPFVTSGIRIGAPAMTTRGFGETEARQLANLIADVLDKPND
                     DANLSNVAEQVKALCARFPVYGK"
     gene            complement(6774..>7954)
                     /locus_tag="PKN55_07890"
     CDS             complement(6774..>7954)
                     /locus_tag="PKN55_07890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012697532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNX71203.1"
                     /translation="TSGTTGKPKGVQRDTGGYAVALAASMKHIYCGNPGEAYFSTSDI
                     GWVVGHSYIVYGPLIGGMATVVYEGLPIRPDPGIWWQIVEKYKVTVMFSAPTAVRVLK
                     KQDPAWLKKYDISTLRYLFLAGEPLDEPTAQWISDALNVPIIDNYWQTETGWPMLTAM
                     PGVENTPVKFGSPSFPAYGYNLKLLDEVSGEEVGPGEKGVVAVLPPLPPGCLSTVWGQ
                     DDRFVNTYFKGFKDKLVYSSFDWGLKDEDGYYFILGRTDDVINVAGHRLGTREIEEAV
                     NTHNSIAECAVVGVADQLKGQVPMAFAVVKDPAQLADETLRASLAKEVIATVSKELGA
                     IANPSQVHFVTQLPKTRSGKVLRRSIQAIAEGRDPGDLTTLDDPSGLEQVKQAVTGQS
                     "
BASE COUNT         1507 a         2561 c         2398 g         1488 t
ORIGIN      
        1 gccggttgcc accactgcgc ctgcacccag tccagccctt cgcccagccc ttcttgcatg
       61 ggcgtgccca acagataggt ggcctgaaaa atcagcgcca tcagcacggt gaacatcggc
      121 agccccagcc aggggtgcaa caagagttgg tcgatgcggg tggtccaggt ggccggcagg
      181 gtggctggca catggtgaat gcccgccagc atggcgcgcg cttcttccag gccggcaggc
      241 agggcatcca gcgcagcaat cgccaccggc gcctcgcggg ccggaatctg cgccgccaat
      301 tgcgtcagcg ccgcgcgcac cgccggccag ttttcgccct tgcgcgcgct catcgccagc
      361 accggcgcgc ccagacgggc ggacaaggca tccaggtcgg cgcgaatgcc cagctggcgc
      421 atttcgtcct gcatattcac cagcaccacc agcggcaagt ccagttgcgc cagttgccac
      481 accagccgcc aatggcgatc cagctgcgcg ccgttgacca ccaccaccaa tccatcgaac
      541 acgctgccgg ccagcacctc gcgcactacc gcttcgtctt cgctgtagcc gctcaggtca
      601 tgaatgcccg gcaggtccac cacttccacc gcgcgcgcgc ccagcaccac cttggcgctg
      661 gccaggtcga tggtcagccc cggccaattg cccacccgct gcgacagccc ggtcagccgg
      721 ttgaaaaacg tcgatttgcc ggcattgggc aagcccacca ccgccagccg ccgaccttgc
      781 cgcgcggctt caatggcgga cagacccgca ccggatggcg gacacttcgg acaactggcc
      841 gtcatgctta cgccccttgc agctcgacgc ggatgccttc ggccagcagc cggcgcaaca
      901 gcagttgggt ggcgcccacc gccacatgca gtggcccgcc cagcgccgcc cggcggaaca
      961 cttgcacctg tgcgccaggc aaaatgccaa aagcggacaa acgctggcgc tcggtgtcgg
     1021 gcagatcaat ggccaccacc tgggcgcgat ggtgggcagc aagcgtggca agcgaagtca
     1081 tgtgggctcc ttacgcggaa agatgttgca tcacattctc agataatacg ctgaatgccg
     1141 ggttttgggc actgcttgcg ccgcgcaaca gcagcaatcc aagcgcggct tgatctcgga
     1201 tcagtggatt catgcggcgc cagcgcattg cagcgcaaca acacagccag caacaagtgc
     1261 gccagtttgc tggccttaca aaacagcatg aatacctgtt agactgtcag gttaatgttt
     1321 ttagcaccac actctgtaga cgccactccg cgcggccctg gcggccaggc aggatgtggc
     1381 gcgttttccc caattctcgt agcgcaggcg cacatgagca tcaaatcgga caaatggatt
     1441 cgccggatgg cgacaacaga cggcatgatc gagccattcg agcccaatca ggtgcgcgag
     1501 gtggatggcc gcaaaattgt gtcctacggc acctccagct atggctacga tatccgctgc
     1561 gccaacgaat tcaagatttt caccaatatc aacagcacca tcgttgaccc gaaaaatttt
     1621 gaccacaaca attttgtcga cttcaccggc gacgtttgta tcattccgcc caattcgttt
     1681 gccctggcgc gcacggtgga atacttccgc atcccgcgca atgtgctcac cgtctgcctg
     1741 ggcaagagca cctacgcccg ctgcggcatc atcgtcaacg tcaccccgtt cgagccggaa
     1801 tgggaaggct atgtgacgct ggagttcagc aacaccacgc cgctgccggc caagatttac
     1861 gctggcgaag gctgcgcgca agtgctgttt tttgaatccg acgaagtgtg cgaaaccagc
     1921 tataaagatc gcggcggcaa gtaccaaggc caggtgggcg tcaccctgcc caagacctga
     1981 gcgcggccgc tggccgtctg ccgcagcgag caaatcgtcg gcgcaaattg tccgccgccg
     2041 tcccggtcgt ggtctatgct gggtaaagaa tctcctgagt tgggtccacg actgaatggc
     2101 gcatccgccc cccttgcggc tgcgccattt tttttagagt cgctcacaaa gctcagcgca
     2161 gcgatcctgg ctaggcgtgc agccgcaggc agtacaagaa gtacgaccag gctgcacaac
     2221 aacgccagga gggttttgtg agtggctcta taattgcaaa ttcacggatt tccgattgac
     2281 gcacagtgtg tgcgtcttat aattcgcccc cattgttgtt tccgtttttt gccatgcagc
     2341 acgtcggccg gtgcccgacg cgccaagctg gctggaggtc atcatgacaa gccgtgtact
     2401 tactgccgtt tcggccctgt atcaaccctc attcttgcaa catattcctg tcacctaggt
     2461 ttctggagac cccgatcaat ggaccgaatc gctgccgacg cactcgccgc cgcccctgaa
     2521 ctctctctcg actttgcccg cgtcaaggcc caactggcca agggctacga aaaacccttc
     2581 ctcatcctcg atacgcaaat cgtgcgtgaa aaatgccggc gcttttttgc cgccatgccg
     2641 cgcgtgcgcc cgcacttcgc cgtgaaatcc aacccggatc aacgcgtgct gcaagtgctc
     2701 aaggaagaag gcgtcggttt tgaaatcgcc tcgatcgccg agctggatca gctgctggcg
     2761 ctcggcgtgc cgccggcgga aattttctac agcaacccga tgaaatcccg cgcgtatgtc
     2821 gaatacgccg tggccaaggg tgtcgagtgg ttcgtcattg attcggtgga agaactgcaa
     2881 aaggtgcatc gcgcctcgcc gaccgccaaa acctacctgc gcatctacac caccaatgtc
     2941 ggcagcgact ggccgctgtc tggcaaattt ggcgcctacg aaagcgaaat ccgcgacatc
     3001 atcgccgaag ccgcgcgcct gggcgccgat ctggccggcg tgaccttcca cgtgggctcg
     3061 caatgcctga acccggaaaa ctggaaagtc ggcctggaaa ccacccgcgc ggtgttcgac
     3121 cagatgctgg ccgccggcct gaaaccgcgc ctggtgaata tcggcggcgg ctacccggtg
     3181 acccacacca aggacatccc gaccatcgcc gaaatcggcg ccgtggtgaa cgccggcctg
     3241 gccgcgtttg gcgacgaggt gcaggtgatt ggcgagccgg gccgttatct ggtgtctgac
     3301 gccggctact ttgtctgccg cgtggtgggc accgccaccc gcgaaggcaa gcgctgggtg
     3361 tacttcgacg ccggcatgtt cggcggcatc atcgaaacca ccgaaggcct gaagtacaat
     3421 atcctgaccg accgcagcgg cccggaaatt atctggaccg tggctggccc gacctgcgat
     3481 tcgctggaca tctgcacccg cgatcaggcg ctgccgcaag acctggtgga agacgacttt
     3541 atctacatca agaacgccgg cgcctacacc accgcctatg ccagcacctt taacggcttc
     3601 ccgctgccgg atgtgatcat cctgtaagcc gccgtgaatc ccgccgtcgc ccccgccagc
     3661 cttgcgctgc cgggggcgac ggtgttttaa gcgctgttaa gcgcgccatt cgcaccagac
     3721 atgccgacgc aagggactgt ccgccgccag cgccggatgg tcaaattcgc cctgttccag
     3781 catgcccagc cgcgccatca aggcgcgcga gcgcagattg cccagcgcgg tgaacgccac
     3841 cacccgcgac atacccaatt cgcgcgccgc cgccagcgcc agttgcgcgc cctcatgggc
     3901 cagcccttgc ccccaggccg gccgagccag ccgccacagc atttccaccg ccggcgcaaa
     3961 cgggaaactg gccgccaccg ggttcagccc gacacagccc aacagcgcat catcatcgcg
     4021 ccgcgccaac acccacgcgc cccagccgcg ctcagccaag ccggcgcgca agcgggcggc
     4081 caatgcgtca ctgtcactgc ggctcagcgg cgccggaaaa tgcgccatca cctgcgggtc
     4141 ggcattcaag gcggcaaaag ccggcaaatc ctcatcacgc cagtggcgca gatgcacccg
     4201 tggtccgctc agcggtaaag ctgtgtgcat gttcaatctc ctgccgcccc tttcggccag
     4261 gcggaacttt tgtctgtcat tggcgctcgt gactggatat gccatgcatg cgcatggtgc
     4321 ttcaggagcg cttatgttga aaaaactgct ggcctcgatt ggtgtaggca gcgcacaggt
     4381 agatacccag attgacaacc cggagctggc ccctggccaa ttattgcaag gccaggtgct
     4441 ggtgcagggc gggcaatccg cgcaggatat cgaaaaaatc gagctggtgc tgatgactca
     4501 ggccgaagac gaaagccagg acagcgaacg caccgtagcc tggcccctat gccgtctgcc
     4561 ggtgtgcggg ccgttcacca tcggtgctgg tgagacccgg tattttccgt ttcagatgac
     4621 cttgccgctg gaaaccccgg tcaacgccct gcgcacccgt gaccgccgtg tgccgctggt
     4681 gtggattcac accgacctgg cgattgccgc cggggtggac agcgacgatc gcgatcaatt
     4741 gctggtgcgg cccggcatgg cgctgcaaac cctgctggac gcctttgccc ggctggattg
     4801 gtatgtgcac agctcggacg tggagctggg ctcggcacga gtggggccga tcagcagcac
     4861 gctgggctgc tatcaggaaa tcgagctgcg cccgcatggc ggcagctggc gcattgaaga
     4921 aatcgagctc acctgcctgt gcgatggcca ccacacccat gtgctgattg aggtggatca
     4981 tcgtctgggt ggagatcact tctatagcct ggtgatgggc ccggactttg cccgccgcga
     5041 ctgggtggct attctgcgcg acaccttgcc cttgtaagag ccgctaacaa aacccgcctg
     5101 gcgttgttgt gtgaccttgc cgtactacct gtactgtcta cggtcacacg cctagccagg
     5161 cgcgcttcgc tgggttttgt tagcgactct aagcaccgct cacaaaaccc gccaggcaaa
     5221 cagcaaaaag cccgcatcac tgcgggcttt ttgctgcggc gtcgctgaat acgggcgacg
     5281 attacttgcc gtacaccggg aagcgggcgc acagggcttt cacctgttcg gccacattgc
     5341 tcaggttggc gtcgtcgttc ggcttgtcca gcacgtcggc gatcaggttg gccagctgac
     5401 gggcttcggt ttcgccaaag ccacgggtgg tcatcgccgg ggcgccgatg cggatgccag
     5461 aggtgacgaa cggtttttcc gggtcgttcg gaatggcgtt cttgttgacg gtgatgtggg
     5521 cgcggcccag ggcggcttcg gcgtccttgc cggtgatttg cttggcgcgc aggtccagca
     5581 ggaacacatg gctttcggtg cggccagaca caatgcgcag gccgcgcttg atcagctctt
     5641 cggccatggc gctggcgttc accttgactt gcttggcgta gaccttgaag tcatccgagg
     5701 cggcttcttt gaaagcaacg gccttggcgg cgatcacgtg catcagcggg ccgccttgca
     5761 ggcaggggaa gatggccgag ttcaggattt tttcgtgttc ggcgcgagcc agaatcacgc
     5821 cgccgcgcgg gccgcgcagg gtcttgtggg tggtggtggt cacgaagtcg gcaaacggca
     5881 ccgggttcgg gtattcaccg ccggccacca ggcctgcgta gtgggccatg tcgacaaaca
     5941 gataggcgcc cacttcgtcg gcgatggcgc ggaagcgcgc ccagtcgatt tccagcgcgt
     6001 aggcagacgc gccggcgaca atcatcttcg gcttgtgttc gcgggccaga cgggccactt
     6061 cgtcgtagtc gatggcttcg gcttcgttca ggccgtaggc caccacgtta tacagcttgc
     6121 cggagatatt gaccgaggcg ccgtgggtca ggtggccgcc atgcgccagg ctcatgccca
     6181 ggatggtgtc gcccggcttg agcaccgaca catacaccgc ctggttggct tgcgagccgg
     6241 aatgcggctg cacattggcg tattcggcgc caaacagggc tttcaggcgg tcaatcgcca
     6301 gttgttcgac ctggtcaacg tattcgcaac caccatagta acgcttgccc ggataaccct
     6361 cggcgtactt gttggtcagc accgagcctt gtgcttccat cacggcgggg ctgacgtagt
     6421 tttccgaggc gatcagttcg acatggtctt cctgacgacg gaactcggcg tccatcatcg
     6481 cggcgagttc gggatcgtag gcatgaatgg tctggtcggc agcaaacatt gtttggattc
     6541 cagttcggtg gagaaaaaag atgaaacgaa ttttacacga cttgccgtaa acggggccat
     6601 gaaatggcga caccccgaca tgaaaacgct tcatcatgcc ggggtgtggc gggtggacgc
     6661 acgcatccac ccaaggggag agcaagagcc gctgacaaac tcagcgccgc gaccctggcc
     6721 aggccgcgca acaacgccag gcgggtttgc tgggcggcgc taaaccgcgc gggtcaggac
     6781 tggccggtga ccgcctgctt gacctgctcc aggccgctcg gatcgtccag cgtggtcaaa
     6841 tcgcccggat cgcggccttc ggcaatcgcc tgaatcgaac ggcgcagcac cttgcccgag
     6901 cgggttttcg gcagctgggt gacaaagtgc acctgcgagg ggttggcaat cgcgcccagc
     6961 tctttgctga cggtggcgat cacttccttg gccaggctgg cgcgcagggt ttcgtcggcc
     7021 agctgggccg ggtctttcac cacggcgaac gccatcggca cctggccctt gagctggtcg
     7081 gccacgccca ccaccgcgca ctcggcgatg gaattgtggg tgttgaccgc ttcttcgatt
     7141 tcgcgggtgc ccagacggtg gccggcgacg ttgatcacat cgtcagtgcg gcccaggata
     7201 aagtaatacc cgtcttcgtc cttcaggccc cagtcaaacg aggagtacac cagcttgtcc
     7261 ttgaagccct tgaagtaggt gttgacgaag cggtcatcct gaccccacac ggtggacagg
     7321 caacccggcg gcagcggcgg cagcaccgcc accacgcctt tttcgcccgg accgacttct
     7381 tcgccgctga cttcgtccag cagcttgagg ttgtagccat aagccgggaa ggacgggctg
     7441 ccaaacttca ccggggtgtt ttccacgccc ggcatggcgg tcagcatcgg ccagccggtt
     7501 tcggtctgcc agtagttgtc gatgatcggc acattcagcg cgtcggaaat ccattgcgcg
     7561 gtcggctcgt ccagcggctc gccggccagg aacaaatagc gcagggtgga gatatcgtat
     7621 tttttcagcc aggccgggtc ctgttttttc agcacgcgca ctgcagtcgg cgcgctgaac
     7681 atcacggtga ctttgtactt ctcgacaatc tgccaccaga tgcccggatc cgggcgaatc
     7741 ggcaggcctt cgtacaccac ggtggccatg ccgccaatca gcgggccgta gacaatatac
     7801 gagtggccca ccacccagcc gatgtcggag gtggagaaat acgcctcgcc cggattgccg
     7861 cagtagatat gcttcatcga cgccgccagc gccaccgcat agccgccggt gtcgcgctgc
     7921 acgcccttgg gcttgccggt ggtgccggag gtgt
//
LOCUS       DAOPYX010000131         7915 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_10978_length_7915_cov_10.682697, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000131 DAOPYX010000000
VERSION     DAOPYX010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 7915)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7915)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7915
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10978_length_7915_cov_10.682697"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(2..709)
                     /locus_tag="PKN55_07895"
     CDS             complement(2..709)
                     /locus_tag="PKN55_07895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012696062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HNX71204.1"
                     /translation="MSTLFLIPTPLGDAPVEQWMLPGQAERLRPLTHFVVEAAKTARK
                     HLKQLALATPLQQLQLTELNEHTPERELAALLAPLDAGHDMGLMSEAGCPAVADPGAG
                     LVRLAHARGHVVEPLIGPSSLLLALMASGANGQRFAFHGYLPVGEGERVAALRKLEQE
                     SRERDCAQLFIETPYRNQQMLSVLRHTLKPHTLLTAACDLTLPTQTIISRTVRDWPTP
                     GPDLHKRPAIFVLYAGK"
     gene            complement(706..1290)
                     /locus_tag="PKN55_07900"
     CDS             complement(706..1290)
                     /locus_tag="PKN55_07900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003824260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family nucleotide pyrophosphatase"
                     /protein_id="HNX71205.1"
                     /translation="MTPLVLASTSRFRRELLARLGLPFTAVAPVCDETPLPGESAADT
                     ASRLASVKARSLIGAHPQALIIGSDQVALLDGQQLGKPGTPERAAAMLRATAGRTLEF
                     HTALSLVNAANGHEQRHTDITRVRMRPLSDAQIQAYLAREPDALLCAGAAKSEGLGGA
                     LIAGIDSSDPNALIGLPLFALVDMLLAEGVEVLA"
     gene            1350..1844
                     /locus_tag="PKN55_07905"
     CDS             1350..1844
                     /locus_tag="PKN55_07905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012696060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceD family protein"
                     /protein_id="HNX71206.1"
                     /translation="MSDPILIDSLDFANQRRHMAGAIALVDLPRTHDLLAHTEGELSW
                     SLEGGKDVLARPTLHLRLAGEFSLVCQRCLTPMPFRLEADSTLTLFTNEARLESACED
                     DDTLDAVMADPAFDVLALIEDEVLLGLPMAPRHAHCEADIRPAGSGKPNPFAALAALK
                     RKPE"
     gene            1872..2051
                     /gene="rpmF"
                     /locus_tag="PKN55_07910"
     CDS             1872..2051
                     /gene="rpmF"
                     /locus_tag="PKN55_07910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HNX71207.1"
                     /translation="MAVQQNKKSPSKRGMHRAHDFLTNPALAVEASTGEVHLRHHISP
                     NGFYRGRKVVKTKGE"
     gene            2302..3327
                     /gene="plsX"
                     /locus_tag="PKN55_07915"
     CDS             2302..3327
                     /gene="plsX"
                     /locus_tag="PKN55_07915"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018747100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HNX71208.1"
                     /translation="MTISIAVDAMGGDVGVGVTVPAALAFLKLHPDTRLILVGRPEAI
                     EAELKRHDQGLRERLSIHPASEVVGMDESPQLALKNKKDSSMRVAITLVKEGLAQACV
                     SAGNTGALMATARFVLKTIPGIDRPAIAKLMPSVRAETCVLDLGANVDCTPEQMLQFG
                     IMGATLMADAKGRANPRVGLLNIGSEDIKGNGAVKQAGELLRQSNLNFVGNVEGNDIY
                     SGAVDVVVCDGFTGNVALKTSEGLAHMVTTFLREEFTRNWFTRVCALFALPVLARFRR
                     RVDSRRYNGASLLGLRGIVVKSHGGTDSLGFRCALEQAREEALADVIQHITEQVAGQL
                     KAAATEA"
     gene            3330..4289
                     /locus_tag="PKN55_07920"
     CDS             3330..4289
                     /locus_tag="PKN55_07920"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004283536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HNX71209.1"
                     /translation="MIYARIAGTGSYLPEQVLTNAEIAQRLDTSDEWIVSRTGIRARH
                     IAAPEQKTSDLALIASQRALEAAGVQAADIDLIVVATSTPDMIFPSTACILQAKLGAH
                     GCPAFDVQAVCAGFIYALATANAYIRSGMARTALVVGAEIFSRILDWNDRTTCVLFGD
                     GAGAAVLTASEEPGILGVELNADGRHQPMLNVPAQVANGEIVGTPYLHMDGGAVFKFA
                     VKTLSDCASKALAAQNMSHEQLDWLVPHQANVRIIESTAKHLHLPMDKVVLTLPEQGN
                     TSAASIPLALDVAVRDGRIQPGHTVMLEGIGGGFAWGAALVKM"
     gene            4429..5355
                     /gene="fabD"
                     /locus_tag="PKN55_07925"
     CDS             4429..5355
                     /gene="fabD"
                     /locus_tag="PKN55_07925"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012696056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HNX71210.1"
                     /translation="MAFAFLFPGQGSQSLNMMDGFAAAVVKDTFDEASDALGDDLWAM
                     LKADSNEAINQTINTQPLMLAAGVAAFRAWLAAGGARPSVVAGHSLGEYSALVAADAL
                     DFDDAVRLVRLRAEAMQSAVPAGEGAMAAVLGLEDADIVAACAEAAQGEVVEAVNFNA
                     PGQVVIAGGKAAVERAIAACKARGAKRAMPLPVSVPSHCALMKPAAERLAEALESVSI
                     NVPVLPVLHNADVAAYSEAAQIRDALVRQLYSPVRWTETIRKLAADGVLLHAECGPGK
                     VLAGLNKRIESTLSTSALTDDAAIAAALTSLA"
     gene            5408..6148
                     /gene="fabG"
                     /locus_tag="PKN55_07930"
     CDS             5408..6148
                     /gene="fabG"
                     /locus_tag="PKN55_07930"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HNX71211.1"
                     /translation="MQFNGQVALVTGASRGIGAAIAETLAQAGAKVIGTATTADGAAA
                     IDARLAPLGGAGVAFNVTGGAEAVDALISDVETRFGPVAILVNNAGITRDQLLMRMKD
                     EDWDAIMDTNLKSVYRLSKAVLRGMMKARSGRIINIASVVGVSGNAGQCNYAAAKAAI
                     IGFSKSLALEVGSRGITVNSVAPGFIDTDMTRALPEAQRAALVNQIALGRLGEAQDIA
                     DTVAFLASAQAKYITGQTLHVNGGMLMP"
     gene            6288..6524
                     /gene="acpP"
                     /locus_tag="PKN55_07935"
     CDS             6288..6524
                     /gene="acpP"
                     /locus_tag="PKN55_07935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HNX71212.1"
                     /translation="MENIEARVKKIVAEQLGVNEAEVKNESSFVDDLGADSLDTVELV
                     MALEEEFGCEIPDEEAEKITTVQQAIDYVSGHQG"
     gene            6676..7914
                     /gene="fabF"
                     /locus_tag="PKN55_07940"
     CDS             6676..7914
                     /gene="fabF"
                     /locus_tag="PKN55_07940"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012696053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HNX71213.1"
                     /translation="MARRRVVITGLGLVSPVGNDVATGWANIVAGKSGITRISKFDAQ
                     AMACQVAGEVRDFDVSAYIPAKDARRMDVFIHYGMAAGIQAVRDAGLESADLDPERVG
                     VIIGSGIGGLPMIEDTHNAYLAGGPRKISPFFIPGSISNMVSGNLSILFGYKGPSYAI
                     VSACTTATHSIGDAARLIEYGDADVMVAGGTEATICPLAIGGFSSAKALSTRNDDPAT
                     ASRPWDAGRDGFVMGEGAGVMVLEEYEHAKKRGATIYAELAGYGMSSDAHHMTAPCED
                     GDGAMRCMKNALKNAGMNPDEVDYVNAHGTSTPLGDVAETVAVKRCFGEHAYKLAVSS
                     TKSMTGHLLGAAGGIEALFSALAVYHQILPPTINLNQPGEGCDLDYVPNTARDAKINV
                     ALSNSFGFGGTNGTLIFKRI"
BASE COUNT         1434 a         2496 c         2597 g         1388 t
ORIGIN      
        1 tttacttccc cgcatacaac acaaaaatcg ccggccgctt gtgcaagtcc gggccgggcg
       61 tcggccagtc gcgcaccgtc cggctgatga tggtttgtgt gggcagggtc aggtcacagg
      121 cggcggtcag cagggtgtgg ggcttcagcg tgtgccgcag cacgctcagc atctgctggt
      181 tgcggtacgg cgtttcgatg aacaactggg cgcagtcgcg ttcgcgggat tcttgctcca
      241 gcttgcgcaa ggcggcgacg cgttcgccct cgcccaccgg cagatagcca tgaaaggcaa
      301 agcgctggcc attggcgccg gaggccatca gcgccagcag cagcgaggag gggccaatca
      361 gcggctcgac cacatggccg cgcgcgtgcg ccagccgcac caggccagcg ccggggtcgg
      421 ccactgccgg gcagccggct tcggacatca ggcccatatc gtggccggcg tccagcgggg
      481 ccagcagcgc ggccagttcg cgctcggggg tgtgttcgtt cagctcggtc agttgcagtt
      541 gttgcagcgg ggtggccagc gccagttgct tgaggtgttt gcgcgcggtt ttggcggctt
      601 ccaccacaaa atgggtgagc gggcgcaggc gttcggcctg gccgggcagc atccattgct
      661 ccactggcgc gtcgcccagc ggggtgggaa tcaaaaacaa ggtgctcatg ccagcacctc
      721 gacgccttcg gccagcagca tgtccaccag ggcaaacagc ggcaggccga tcagcgcatt
      781 ggggtcgctg ctgtcgatgc cggcaatcag cgcgccgccc aggccttcgc tcttggccgc
      841 gccagcgcac agcagcgcgt ccggctcgcg ggccagatag gcctgaatct gcgcgtcgct
      901 caaggggcgc atgcgcacgc gggtgatgtc ggtgtggcgc tgttcgtggc cgttggcggc
      961 gttgaccagg ctgagcgcgg tgtgaaactc cagcgtgcgc ccggcggtgg cgcgcagcat
     1021 ggcggcggcg cgctcgggcg tgccgggctt gcccagttgc tggccatcca gcagcgccac
     1081 ctgatcagag ccgatgatca gcgcctgtgg atgggcgcca atcaacgagc gcgccttgac
     1141 cgaagccagc cggctggcgg tgtcggcggc gctttcgccg ggcagcgggg tttcgtcgca
     1201 cacgggggcg acagcggtaa aaggcaggcc caaacgggcg agcagttcgc gacgaaagcg
     1261 cgaggtggag gcgagcacga gcggcgtcat ggcgggagtg cttgatttct ttgacacaag
     1321 acgggcgcgg attatatcat gcgcggttta tgtctgaccc tattttgatc gacagcctcg
     1381 acttcgcgaa tcagcgccgg cacatggccg gggccatcgc attggtcgac ctgccccgca
     1441 cccatgatct gctcgcccac actgagggcg agttgagctg gtcgcttgaa ggcggcaagg
     1501 acgtgctcgc gcgtcccacg ctgcatctgc gcctggcggg tgagttttcg ctggtgtgcc
     1561 agcgttgcct gacgcccatg ccgtttcggc tggaggcgga cagcacgctg accttgttca
     1621 ccaacgaagc gcggctggag tcggcctgtg aagacgacga cacgctggac gccgtgatgg
     1681 ctgacccggc gttcgacgtg ctggccctga tcgaagatga ggtattgctg ggtttgccga
     1741 tggcgccgcg ccacgcacat tgcgaagcgg acatccggcc ggccggcagc ggcaagccta
     1801 acccgtttgc cgcgctggct gcgctcaagc gcaagcccga gtaaattttt taagatttca
     1861 aggagtgaat catggccgtt caacagaaca aaaagtcccc gtccaagcgc ggcatgcatc
     1921 gtgcccacga ctttctgacc aacccggcgc tggccgtgga agcctccacc ggtgaagtgc
     1981 acctgcgcca ccacatcagc ccgaacggtt tctaccgtgg ccgcaaagtg gtgaagacca
     2041 agggcgaata atcgcttgcc cgtcttgcca tgcagacatc aagccgtgcg gtttccgcac
     2101 ggcttgcttg ctttcggtcc gtcatgcgcc attgtcatga gagatggcgg attaagccgg
     2161 gcagacgggg tcagaagcca tgcgcggcgc gctcgcgcct gagggcgcaa atttgtacac
     2221 tgtcgttttt gtcatcctga ccgttgctgc ggcccgccct gaggcgaggc gcatgttcaa
     2281 gcggctttcc ttgttgatcc aatgaccatt tccattgctg tggacgccat gggcggcgat
     2341 gtgggcgtgg gcgtcaccgt gccggcggcg cttgcctttc tcaagctgca cccggacacc
     2401 cgcctgattc ttgtcggccg gcccgaagcc attgaggccg aactcaagcg ccacgaccaa
     2461 ggcttgcgcg agcgcctgtc gattcacccg gccagcgagg tggtgggcat ggatgagtct
     2521 ccgcaactgg cgctgaaaaa caaaaaagat tcatcgatgc gggtggcgat caccctggtc
     2581 aaggaaggcc tggcgcaggc gtgcgtgtcg gccggcaaca ccggcgcgct gatggccacc
     2641 gcccgctttg tgctgaaaac cattcccggc atcgaccgcc cggccatcgc caagctgatg
     2701 ccctcggtgc gcgcagaaac ctgcgtgctg gacctggggg cgaatgtcga ttgcacgcct
     2761 gagcaaatgc tgcaatttgg catcatgggc gccacgctga tggccgacgc caagggccgc
     2821 gccaatccgc gcgtgggcct gctcaatatt ggctcggaag acatcaaggg caatggcgcg
     2881 gtcaagcagg ctggcgaatt gctgcgccag tccaacctga attttgtcgg caatgtcgaa
     2941 ggcaacgata tttacagcgg cgcagtggac gtggtggtgt gcgacggctt caccggcaat
     3001 gtggcgctga aaacctcgga aggcctggcg catatggtga ctaccttcct gcgcgaggaa
     3061 ttcacccgca actggttcac ccgcgtgtgc gcgctgtttg ccctacccgt gctggcgcgc
     3121 tttcgccgcc gggtggattc gcgccgttac aacggcgcca gccttttggg cctgcgcggc
     3181 attgtggtaa aaagccacgg cggcacggac agcctgggct ttcgttgcgc actggaacag
     3241 gcgcgtgaag aagcgctcgc cgacgtcatc caacacatca ccgaacaggt ggccggccag
     3301 ttgaaagccg ccgccacgga ggcctgacca tgatttatgc ccgcattgca ggcacgggca
     3361 gctatttgcc agaacaggtg ctgaccaacg ccgagattgc ccagcggctg gacaccagcg
     3421 acgaatggat tgtcagccgc accggcatcc gcgcccgcca tatcgccgcg ccagaacaaa
     3481 aaaccagcga cctggcgctg atcgcctcgc agcgcgcgct ggaagcggct ggcgtgcagg
     3541 cggcggatat tgacctgatc gtggtggcca cttccacccc ggacatgatc ttccccagca
     3601 cggcgtgtat tctgcaagcc aaactgggcg cacatggctg cccggcgttt gacgtgcagg
     3661 cggtgtgcgc cggctttatc tacgcgctgg ccaccgccaa cgcctatatc cgcagcggca
     3721 tggcgcgcac cgcgctggtg gtgggcgcag aaatcttctc gcgcatcctg gactggaacg
     3781 accgcaccac ctgcgtgctg tttggcgacg gcgcaggcgc agccgtgctg accgccagcg
     3841 aggagcccgg cattctgggc gtggagctca acgccgacgg ccgccaccag ccgatgctga
     3901 acgtgccggc gcaagtggcc aatggtgaaa ttgtcggcac gccgtatctg cacatggacg
     3961 gcggcgcggt gttcaagttt gcggtgaaaa ccctgtccga ttgcgccagc aaggcgctgg
     4021 cggcgcagaa tatgagccac gagcagctgg attggctggt gccgcaccag gccaatgtgc
     4081 gcattatcga atccaccgcc aagcatctgc atctgccgat ggacaaagtg gtgctcaccc
     4141 tgcccgagca aggcaacacc tcggccgcgt cgattccgct ggcgctggac gtcgccgtgc
     4201 gcgatggccg cattcagccg ggccatacgg tgatgctgga aggcattggc ggcggttttg
     4261 cctggggcgc cgcgctggtg aaaatgtaag catgccgccc atggggggcg tgtgtgggcc
     4321 atgcgttgcg cgtcggatat tctccggcgc gttgttgtat tggccatcag aatttaaacg
     4381 tatgttcgca tgccggcgca tgcggcttca ggcagaggag gccagcacat ggcttttgca
     4441 tttttgtttc ccggtcaagg ctcgcaaagc ctgaacatga tggatggctt tgccgccgcc
     4501 gtggtcaagg acacttttga cgaagccagc gacgccctgg gcgatgacct gtgggccatg
     4561 ctcaaggccg acagcaacga agccatcaac cagaccatca acacccagcc gctgatgctg
     4621 gccgctggcg tggctgcctt ccgcgcctgg ctggccgcgg gcggcgcccg ccccagcgtg
     4681 gtggccggcc acagcctggg tgaatacagc gccctggtgg cggccgacgc gctggatttt
     4741 gacgacgccg tgcgcctggt gcgcctgcgc gccgaggcca tgcaatcggc cgtgccggct
     4801 ggcgaaggcg ccatggccgc cgtgctgggc ctggaagacg ccgacatcgt cgccgcctgc
     4861 gccgaagccg cgcaaggtga agtggtcgag gcggtgaact tcaacgcccc tggccaagta
     4921 gtgattgccg gcggcaaggc ggcggttgaa cgcgccatcg ccgcctgcaa ggcgcgcggc
     4981 gccaagcgcg ccatgccgct gccggtgtcg gtgccctcgc attgcgcctt gatgaagccg
     5041 gccgccgagc ggctggccga ggcgctggaa agcgtgtcga tcaatgtgcc ggtgctgccg
     5101 gtgttgcaca atgccgacgt ggccgcctac agtgaagccg cacaaatccg cgatgcgctg
     5161 gtgcgccagc tgtattcgcc ggtgcgctgg acggaaacca tccgcaagct ggccgccgat
     5221 ggcgtgctgc tgcacgccga atgcgggccg ggcaaggtgc tggccgggct gaacaagcgc
     5281 atcgaaagca cgctgagcac cagcgcgctg accgacgacg cggccattgc cgccgcgctc
     5341 accagcctgg cctgattcac ccgcaggcgg cgcgccgcct gcggcaaaac cataaggaga
     5401 cacagggatg caattcaatg gacaggtggc gctggtgacc ggcgcttctc gcggcattgg
     5461 cgctgcgatt gctgaaaccc tggcgcaagc tggcgccaag gtgattggca ccgccaccac
     5521 cgccgacggc gccgccgcca tcgacgcccg cctggccccg ctgggcggcg ccggtgtggc
     5581 gtttaatgtc actggcggcg cggaagcggt ggatgcgctg atcagcgacg tcgaaacccg
     5641 ctttggtccg gtggccattc tggtcaacaa tgccggcatc acccgcgacc aattgctgat
     5701 gcgcatgaag gacgaggact gggacgccat catggacacc aacctcaagt cggtgtaccg
     5761 cttgtccaaa gcggtgctgc gcggcatgat gaaggcgcgc agtggccgca tcatcaatat
     5821 cgcctcggtg gtgggcgtgt ccggcaacgc cggccagtgc aactacgccg ccgccaaggc
     5881 cgcgattatt ggtttttcca aatcgctggc gctggaagtc ggcagccgcg gcatcaccgt
     5941 caacagcgtg gcgccgggct ttatcgacac cgacatgacc cgcgccctgc ccgaagcgca
     6001 gcgcgccgca ttggtcaacc agattgccct gggccgtctg ggcgaagcgc aagacattgc
     6061 cgacaccgtg gcttttctgg cctcggcgca ggccaaatac atcaccggcc agacgctgca
     6121 cgtcaatggc gggatgttga tgccgtaaag acaatggcaa ttttgcgcca cacttcgctt
     6181 gatctgatgt caggctgacg caagtggcgc taggtggcct tcaggttttt ttgttagaat
     6241 gccggggttt ttggttaccc caacacgaaa ggtaagggtt tacacaaatg gaaaacatcg
     6301 aagcacgtgt caagaagatc gtggccgagc aactgggcgt gaacgaagct gaagtgaaga
     6361 acgaatcttc cttcgtcgac gatctgggcg cggattcgct cgacaccgtc gaactggtga
     6421 tggcgctgga agaagaattc ggctgcgaaa tcccggacga agaagctgaa aagatcacca
     6481 ccgttcagca agccatcgac tacgtgagcg gccaccaagg ctaaggctcg cgttgccgcg
     6541 caagtggcag acagtcgtcc agacgcccgc cggcaacccg gccggcgtct gtcgcgtctt
     6601 ccggtgtttt tgtttgagcg tgtgcatgca ctttcccgca aacacaccgt tttgttcctc
     6661 agtaaggagt gcatcttggc aagacgcaga gtcgtcatca ccggccttgg tctggtgagc
     6721 ccggtcggca acgatgtcgc aaccggttgg gccaacatcg tcgccggcaa atccggcatc
     6781 acccgcatca gcaagttcga cgcgcaggcc atggcctgcc aggtggccgg cgaagtgcgt
     6841 gacttcgatg tcagcgccta cattcctgcc aaggatgccc gccgcatgga cgtgttcatc
     6901 cattacggca tggcggccgg catccaggcc gtgcgcgacg ccgggctgga aagcgcagac
     6961 ctcgaccccg agcgcgtcgg cgtgatcatc ggctccggca ttggcggcct gccgatgatt
     7021 gaagacaccc acaacgccta tctggccggc ggcccgcgca agatctcgcc gttcttcatc
     7081 cccggctcga tcagcaatat ggtgtcgggc aatctgtcca tcctgtttgg ctacaaaggc
     7141 cccagctacg ccatcgtgtc ggcctgcacc accgccaccc attcgattgg cgatgctgca
     7201 cgcctgatcg aatacggcga cgctgacgtg atggtggccg gcggtactga agccaccatc
     7261 tgcccgctgg cgattggcgg tttctcctcg gccaaggccc tgtccacccg caacgacgac
     7321 ccggccaccg ccagccgtcc gtgggacgcc ggccgcgatg gctttgtgat gggcgaaggc
     7381 gccggcgtga tggtgctgga agaatacgaa cacgccaaaa agcgtggcgc caccatctac
     7441 gccgaactgg ccggttatgg catgagctcc gacgcccacc acatgaccgc cccgtgcgaa
     7501 gacggcgacg gcgccatgcg ctgtatgaag aacgcgctga aaaacgccgg catgaacccg
     7561 gacgaagtgg actacgtcaa cgcccatggc acctccaccc cgctgggcga cgtggcggaa
     7621 accgtggcgg tcaagcgctg ctttggcgaa cacgcctaca agctggcggt cagctcgacc
     7681 aagtcgatga ccggccacct gctgggcgcg gcgggcggta ttgaagccct gttctcggcg
     7741 ctggcggtgt atcaccagat cctgccgccg accatcaacc tgaaccagcc aggcgaaggt
     7801 tgcgatctgg actacgtgcc gaacaccgcg cgcgacgcca agatcaacgt ggcgctgtcc
     7861 aactcgtttg gctttggcgg caccaatggc acgctgatct tcaagcgcat ctaag
//
LOCUS       DAOPYX010000132         7879 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_11060_length_7879_cov_12.740925, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000132 DAOPYX010000000
VERSION     DAOPYX010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 7879)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7879)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7879
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11060_length_7879_cov_12.740925"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            135..887
                     /gene="moeB"
                     /locus_tag="PKN55_07945"
     CDS             135..887
                     /gene="moeB"
                     /locus_tag="PKN55_07945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018746922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HNX71214.1"
                     /translation="MNDQQLLRYSRHILLPQVDITGQTSLLDSHALVIGAGGLGSPVA
                     MYLASAGVGTLTLCDNDVVELTNLQRQIVHDIDSLGQLKVESAARRLGKLNPDVRVQA
                     LAQRMDAAALAEAVAKADVVLDCSDNFVTRHAVNAACVAARTPLVSGAAVRFDGQLAV
                     FDPRQHDAPCYHCLFPDTGDGSDGPCATFGVFAPLVGIIGCMQAAEALKLLLGIASHA
                     VNRLSIYDGLSGDWRHMQLARDPACPVCGGAH"
     gene            1057..1977
                     /locus_tag="PKN55_07950"
     CDS             1057..1977
                     /locus_tag="PKN55_07950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018152835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HNX71215.1"
                     /translation="MDLKWLEDFISLAETHSFSRSAELRHVTQPAFSRRIRSLESWLG
                     VDLIDRTAYPTRLTPAGELFHGQALEIIAQLNHARALLRGQQSAPPDTLNIAVPHNLA
                     LTFFPKWLTEVKRGCGEFSTRLTAGNVHDAVMALESGSCDVLLCYHHPHQPVLLDPAR
                     YDMLALGEETLRPYARRTASGQPAFSLPGSPQQPIPFLAYAAGAYLERMVALMVANGE
                     EILHLTPCYETDMAEGLKSMVLEGHGLAFLPDSAVCGEVERGQLAVAGDARWQLNMEI
                     RLYREHRSSKPLVNMLWQHLRESTVRRRRP"
     gene            2085..2507
                     /locus_tag="PKN55_07955"
     CDS             2085..2507
                     /locus_tag="PKN55_07955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014086708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde-activating protein"
                     /protein_id="HNX71216.1"
                     /translation="MHRLSGQCHCQAIQLELQLPQPPASYQPRACDCDFCRKHAAAYV
                     SDPDGALRIRARPGALGLYRQGSAKAQCVYCTHCGVLIGVMYEAVGHRYATVNVRVLD
                     GEPGFADSVVVSPKTLSAAEKVSRWKQLWCRGVEVEER"
     gene            complement(2671..4215)
                     /locus_tag="PKN55_07960"
     CDS             complement(2671..4215)
                     /locus_tag="PKN55_07960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003783131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HNX71217.1"
                     /translation="MNAPASHPPLSGTKLLLGTVALSLATFMNVLDTTIANVSIPAIS
                     GDLGVSSSQGTWVITSFAVANAIAVPLTGWLTQRFGQVRLFITSVLLFVLASLLCGLA
                     PNLEMLIAFRVLQGAVAGPMIPLSQALLLSSYPPAKSGMALAMWSMTTMVAPVIGPIL
                     GGWLSDNVSWPWIFYINVPFGLLAAWATWRIYQDRDSPTRQLPIDKIGLVLLVLWVGA
                     LQIMLDKGKELDWFNSGEIVALGLVALVSFVLFLVWELTEAHPVVDLSLFTRRNFVTG
                     TLTLSVAYSLFFGGILIIPLWLQTQMGYTATLAGLTAAPVGILALLLSPVIGKLLPRL
                     DARAIASVSFVVLATVSFMRAGFTTGADNFTVMLPQIVLGAGMATLFLPLTAILLSGL
                     HPSRIPAASGLSNFCRITAGAFGASIATTVWEDRTTLHHAQLVEQITPFHPAATQARD
                     GLMGAGLSPEQSLATLDRLVTGQAGVLAANEYFWLSGFLFLTLLALVWLARPQARVAV
                     GPGADH"
     gene            complement(4212..5387)
                     /locus_tag="PKN55_07965"
     CDS             complement(4212..5387)
                     /locus_tag="PKN55_07965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008952216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNX71218.1"
                     /translation="MTDAASAVSSSPAAPGRRKTLLLRIVAGLALAGAAYGAWWGLVA
                     RHYQHTDNAYVGGNLVQITPQTSGTVLSILADDTDFVAAGQPLVKLDPADAEVALAQA
                     EADLAQQVRQSRALFANRAASAALLAQRQSELSRAEDDLKRRQAAGGEAVAGEELAHA
                     SAAVANARAALSAAKEQLAASEAQTENTRPDSHPAVARAAARVRESYLALSRATLPAP
                     VAGYVAKRGVQVGQRVQPGAPLMAIVPLNSLWVDANFKESQLAGMRIGQPVTLHADIY
                     GGSVTYHGKVAGLGAGTGGVFSLLPAQNATGNWIKVVQRVPVRITLDPKELSEHPLRV
                     GLSMLAEVDQHDPSGPALATAPRQAPAQSTTAFAGADQAAQARIARIIADNLGKDRS"
     gene            complement(5384..6805)
                     /locus_tag="PKN55_07970"
     CDS             complement(5384..6805)
                     /locus_tag="PKN55_07970"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HNX71219.1"
                     /translation="MRYVFSCLAAAGALCLTACAGPGAPALPLTPPASLSGPLAQGQA
                     AWPQGAWWRAFADPQLDALIERALADAPSMQLARARLRQAVAVGEQAGAALLPGAALN
                     ADVTRQRYSEHGMVPPPMAGSWRTSGQLSLDFAYDLDLAGGERARRDAAQDRAQAAGA
                     DEAGARLLLSAALTHAYWGLERLHALANLAGESQASAERAAALLGQRQRAGLAHAGEL
                     AAAQSQAAAARQEALALAEQITLQRHAIAALAGQGPALANTLHRPQLRAPAGDDLPTV
                     LPAALLGRRPDIVAARWRVEAGGREVDSARAAFYPNINLLSFIGYSAIGMSQLLQSAS
                     RVAGLGPALSLPLFDGGARRAALAGAEAGRDAQIAQYNQTVLDAVREVADQAASLRAL
                     AAQSRQTDTAQTQAAAARHSAQMRARAGLASELDALALDAPWLAQRRQAVELNTRRQQ
                     ARVGLIKALGGGYASSDFAENAQ"
     gene            6936..7856
                     /locus_tag="PKN55_07975"
     CDS             6936..7856
                     /locus_tag="PKN55_07975"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HNX71220.1"
                     /translation="MSQADLNQLLVFAKVVEHGSFIAASRALGLPKTTVSRKVQELEE
                     RLGARLLQRTTRRVALTEAGAIYHEYCSRIVQDIADADLAVGRVHSAPRGELRVSASF
                     SFGMGALVPIVPDFMARYPDIRLQLELRNDAVDLVAEGFDLAIRIGPLEDSSCAVRYL
                     AESRLALYASPDYLARAGMPATLDEVARRPTLTLSRLGRHGRFYWPLARQNGEARELA
                     ITPQLVANDPGVIKFAALAGLGVALLPVILIRPEVESGQLLPVLPDWEGPPTEIGAVY
                     PSRRGLSPKVRVFIDFLSERLAHLSGSRRG"
BASE COUNT         1325 a         2679 c         2667 g         1208 t
ORIGIN      
        1 tgctccgtgg gcctggcgtt cagtcacgag ggaaagtcgc taatccgggg cgctttccct
       61 ctttttttgc ctgccgctcg aaatcccgcc ctacccgcgc tacagtgccg gttttacctg
      121 ccgctttctc cgccatgaac gaccaacaac tgctgcgcta cagccgccat atcctgctgc
      181 cccaagtaga catcaccggc cagaccagcc tgctggacag ccacgccctg gtgattggcg
      241 cgggcggcct gggctcgcca gtggccatgt atctggcctc ggccggcgtg ggcacgctca
      301 ccctgtgcga caacgacgtg gtggagctga ccaatctgca acgccagatc gtccacgaca
      361 tcgacagcct gggtcagctc aaggtggaat ccgccgcgcg ccggctgggc aagctcaacc
      421 cggacgtgcg ggtgcaggcg ctggcccagc gcatggacgc cgccgcgctg gccgaggcgg
      481 tggccaaggc cgatgtggtg ctggattgct cagacaattt tgtcacccgc cacgccgtca
      541 acgccgcctg cgtggcggcg cgcacgccgc tggtgtcggg cgcggcggtg cgctttgatg
      601 gccaactggc ggtgttcgac ccgcgccagc acgacgcgcc gtgttatcac tgcctgtttc
      661 ccgacactgg cgacggcagc gacggcccat gcgccacctt tggcgtgttt gccccgctgg
      721 tgggcatcat cggctgcatg caggcggcgg aggcgctcaa gctgctgctg ggcatcgcct
      781 cgcacgcggt caaccgcctg tcgatttacg acggcctcag cggcgactgg cggcatatgc
      841 aactggcgcg cgatccggcc tgcccggtgt gtggcggcgc gcactgaccc tgccctgccc
      901 aggcgtgtga gcgcagacag ggcgggcagt acggcaagcg cacaccacaa cgccagaggg
      961 ttttgcgctc gacacggacg cagccattgc cggattgccc gcaagcgact aggctgcatg
     1021 cctttgtgct tcgcgcgttg accacaggct gccccgatgg atctgaaatg gctggaagac
     1081 ttcatcagtc tggccgaaac ccatagtttt tcccgctcgg ccgaattgcg ccatgtcacc
     1141 cagccggcgt tctcccgccg catccgctcg ctggaaagct ggctgggcgt ggacttgatc
     1201 gaccgcaccg cctaccccac ccggctgacc ccggccggcg agctgtttca tggccaggcg
     1261 ctggaaatca tcgcccagct caaccacgcc cgcgccctgc tacgcggcca gcaatccgcc
     1321 ccgccagaca cgctgaacat cgccgtgccg cacaacctgg cgctgacctt ctttcccaag
     1381 tggctgaccg aggtcaagcg cggctgcggc gaattcagca cccggctgac cgccggcaat
     1441 gtgcacgacg cggtgatggc gctggaaagc ggcagctgcg atgtgctgct gtgctaccac
     1501 cacccgcacc aacccgtgct gctcgacccg gcgcgctacg acatgctggc cctgggcgaa
     1561 gaaaccctgc gcccctacgc ccgccgcacc gccagcggcc agccggcctt cagcctgccc
     1621 ggcagcccgc aacagcccat cccgtttctg gcctacgccg ccggcgccta cctggagcgc
     1681 atggtggcgc tgatggtggc caacggcgaa gaaatcctcc acctcacccc ctgctacgaa
     1741 accgatatgg ccgaaggcct gaaaagcatg gtgctggaag gccacggcct ggcgttcttg
     1801 cctgacagcg cagtgtgcgg cgaagtggag cgcggccagc tggcggtggc cggcgatgcc
     1861 cgctggcaac tgaatatgga aatccgcctg taccgcgaac accgcagcag caagccgctg
     1921 gtgaatatgc tgtggcagca cctgcgggaa agcaccgtgc gccgtcgccg gccgtaatgg
     1981 gcgacgggcg cgacagtaca aagcaatacg gccaggccgc gtcaccacgc cgagaggggc
     2041 atgtgagcgg cgcttatcta cgcccacaga aaggcagcgt tcccatgcat cgactcagcg
     2101 gccagtgcca ttgccaggcc attcaactgg aacttcaact gccgcagccg ccggccagct
     2161 atcagccgcg cgcctgtgac tgtgattttt gccgcaagca cgcggcggcc tatgtgtccg
     2221 acccggacgg cgcactgcgc atccgcgccc gccctggcgc gctgggtttg tatcgtcagg
     2281 gcagcgccaa ggcgcagtgt gtgtattgca cccattgcgg ggtactgatt ggggtgatgt
     2341 acgaggccgt gggccaccgt tacgccacgg tgaatgtgcg cgtgctggac ggcgagccag
     2401 gctttgccga cagcgtggtg gtgtcgccaa aaaccctgtc ggctgccgag aaggtatcgc
     2461 gctggaagca gctgtggtgt cgcggggtgg aggtggaaga gcggtagcgg tgaggcgtga
     2521 gcctccccgc tgcactgagt ttggcgggca acccttggag tcgctcacaa aacccggtgc
     2581 agcggtcctg accaagcgcg cggccgcaca ccatacaaag cagtacggca aggccgcgca
     2641 acaacgccag gcgggttttg tgagctgcgc ttaatgatct gcccccggcc ccaccgccac
     2701 cctggcctgt ggccgcgcca gccacaccag cgccagcagg gtaagaaaca aaaaccccga
     2761 cagccaaaaa tactcattgg ccgccagcac tccagcctgg ccggtaacca gccggtccag
     2821 cgtggccagg ctttgctccg ggctgagccc tgcgcccatc aagccgtcgc gcgcttgcgt
     2881 ggctgccgga tgaaacggcg tgatctgctc gaccaattgc gcgtgatgca gggtggtgcg
     2941 gtcttcccac accgtggtgg cgatcgaggc gccaaaggcg cctgcggtga tccggcagaa
     3001 gttggacaag ccggacgccg ccgggatgcg gctggggtgc aggccggaca ataaaatcgc
     3061 cgtcagcggc aaaaacagcg tggccatgcc ggcgcccagc acgatttgcg gcagcatcac
     3121 ggtgaagttg tctgcgccag tggtgaagcc ggcgcgcatg aacgacaccg tggccagcac
     3181 cacaaacgac acgctggcaa tcgcccgcgc atccagccgg ggcaaaagct tgccaatcac
     3241 tggcgagagc agcagggcca aaatgcccac cggcgcggcg gtgaggccgg ccagggtggc
     3301 ggtgtagccc atttgcgttt gcagccacag cggaataatc aagatgccgc caaaaaacag
     3361 cgaatacgcc accgacagcg tcagcgtgcc ggtgacaaaa ttgcgccggg tgaacaaaga
     3421 caaatccacc accgggtgcg cctcggtcag ctcccacacc aaaaacagca caaagctcac
     3481 cagcgccacc aggcccagcg cgacaatctc gccggagtta aaccaatcca gctccttgcc
     3541 cttgtccagc atgatctgca aggcgcccac ccacagcacc agcagcacca ggccaatctt
     3601 gtcgatgggc agctggcggg tggggctgtc gcggtcttgg taaatccgcc aggtggccca
     3661 ggcggccagc aggccaaacg gcacattgat ataaaaaatc cacggccagg acacattatc
     3721 cgacagccag ccgcccagaa tcggcccgat cactggcgcc accatggtgg tcatcgacca
     3781 catggccagc gccatgccgc ttttggccgg cggatacgag ctgagcaaca gcgcctgcga
     3841 cagcggaatc atcggcccgg ccaccgcgcc ttgcagcacc cgaaaagcaa tcagcatttc
     3901 cagattgggg gccagcccgc acagcagcga ggccagcaca aacaacagca ccgaggtgat
     3961 gaacaaacgc acctggccaa aacgctgggt cagccagccg gtcagcggca cggcgatggc
     4021 gttggccacg gcaaacgagg taatcaccca ggtgccctgc gaggaggaca cgcccaggtc
     4081 gccagaaatc gccggaatcg acacattggc gatggtggtg tccagcacat tcataaaggt
     4141 ggccagcgac agcgccaccg tgcccagcag cagttttgtg ccgctcaacg gcggatggga
     4201 cgccggggcg ttcatgagcg gtccttgccc agattgtcgg caatgatgcg ggcaatgcgc
     4261 gcctgcgcgg cctgatcggc gccggcaaag gcggtggtgc tttgcgccgg ggcctggcgc
     4321 ggcgcggtgg ccagcgccgg gccggatggg tcatgttggt ctacttcggc cagcatcgac
     4381 agccccaccc gcagcgggtg ttcgctcagt tctttcgggt cgagggtgat gcgcaccggc
     4441 acgcgctgca ccaccttgat ccagttgccg gtggcgtttt gcgccggcaa cagcgagaac
     4501 accccgccag tgcccgcgcc caggccggcc accttgccgt ggtaggtgac gctgccgcca
     4561 taaatatcgg cgtgcagggt gacgggctgg ccaatgcgca tgccggccag ctggctttct
     4621 ttgaagttgg cgtccaccca cagcgaattc agcggcacga tggccatcag cggcgcgccc
     4681 ggctgcacgc gctggcccac ctgcacgccg cgcttggcca catagccggc caccggggcg
     4741 ggcagggtgg cgcggctgag cgccagatag ctctcgcgca cccgtgcggc ggcgcgcgcc
     4801 accgccgggt ggctgtccgg gcgggtgttt tcagtttgcg cttcgctggc ggccagttgc
     4861 tctttggcgg cgctcagcgc ggcgcgggca ttggccacgg cggcgctggc gtgggccagc
     4921 tcttcgccgg ccaccgcctc gccgccagcg gcttgccggc gcttgaggtc gtcttcggcg
     4981 cggctcagct cgctttgccg ttgcgccagc agcgcggcgc tggcggcgcg gttggcaaac
     5041 agcgcgcggc tttgccgcac ctgctgggcc aggtcggcct cggcctgcgc cagcgccacc
     5101 tcggcatcgg ccgggtcgag cttgaccagc ggctggccgg cggcaacaaa atccgtgtcg
     5161 tcggccagga tgctgagcac cgtgccgctg gtttgcgggg tgatctgcac cagattgccg
     5221 ccgacatagg cgttgtcggt gtgttggtaa tggcgggcaa ccaggcccca ccaggcgcca
     5281 taggcggcgc cggccagcgc caggccggcg acaatgcgca gcagcagggt tttgcggcgg
     5341 ccgggcgcag ccggggagga agaaacagca gaagcagcgt cggtcattgg gcgttctcgg
     5401 caaaatcaga tgaggcataa ccgccgccca gggccttgat caggccgacg cgggcttgtt
     5461 ggcggcgggt gttgagctcc accgcctggc ggcgctgggc cagccacggg gcgtccagcg
     5521 ccagcgcgtc cagctcgctg gccaggccgg cgcgggcgcg catttgcgcg ctgtggcggg
     5581 cggcggcggc ctgggtctgg gcggtgtcgg tttggcggga ttgcgcggcc agcgcgcgca
     5641 ggctggcggc ctggtcggcc acctcgcgca ccgcgtccag cacggtctgg ttgtattggg
     5701 cgatctgggc gtcgcggcca gcttcggcgc cggccagcgc ggcgcggcgg gcgccgccgt
     5761 caaacagcgg caggctcagc gccgggccca gtccggccac ccggctggct gattgcaaga
     5821 gctggctcat gccaatggcg gaatagccaa taaagctcag cagattgata ttggggtaaa
     5881 acgccgcgcg cgcgctgtcc acctcgcgcc caccggcctc cacccgccag cgggcggcga
     5941 caatatccgg gcggcggccc agcagcgcgg ccggcagcac ggtgggcaag tcatcacccg
     6001 ccggtgcgcg cagctgcggg cggtgcaggg tgttcgccaa ggccgggccc tggccggcca
     6061 gggcggcaat ggcgtggcgt tgcagggtga tttgctcggc cagcgccagc gcctcttgcc
     6121 gggcggcggc ggcctggctt tgcgcggcgg ccagctcgcc agcatgggcc aggccggcgc
     6181 gctggcgttg gcccagcagc gcggcggcgc gctcggcgct ggcctggctt tcgccagcca
     6241 gattggccag cgcgtgcagc cgctccaacc cccaataagc gtgggtcagc gcggcggaca
     6301 gcaataagcg cgcgccggct tcatcggcgc cagcggcctg ggcgcggtct tgcgcggcgt
     6361 cgcggcgggc gcgttcgccg ccggccaaat ccaggtcata ggcaaaatcc aggctgagct
     6421 ggcccgaggt gcgccagctg ccagccatcg gcggcggaac catgccgtgc tcgctgtagc
     6481 gctggcgggt gacgtcggcg ttcagcgctg cgccaggcag caaggcggcg ccggcttgct
     6541 cgcccaccgc caccgcctga cgcagccgcg cccgcgccag ctgcatcgac ggcgcatcgg
     6601 ccagcgcgcg ctcgatcaag gcgtccagct gcgggtcggc aaacgcgcgc caccacgcgc
     6661 cttgcggcca cgccgcctga ccctgggcca agggcccgga caggctggcc ggcggggtca
     6721 gcggcaaggc cggcgcgccc ggcccggcgc acgccgtcag gcatagcgcg ccagcggcgg
     6781 caagacaaga aaacacatag cgcatgatag ccaagcatcc aaaacaggaa gcccacactc
     6841 tacgccgccg gccatggcgg gataagccgg caaaaatggg aaacattgtc ctgttgatgg
     6901 gataattgcc gtgttttctt tgccggggtg ggctgatgtc gcaagcagat ctcaaccaat
     6961 tgctggtgtt cgccaaagtg gtggaacacg gcagctttat cgccgcctcg cgcgcgctgg
     7021 gcctgccgaa aaccacggtc agccgcaagg tgcaagagct ggaagaacgc ctgggcgcgc
     7081 ggctattgca gcgcaccacc cgacgggtgg cgctgaccga ggccggggcg atttatcacg
     7141 agtattgcag ccgcatcgtg caggacatcg ccgacgccga cctggctgtg ggccgcgtgc
     7201 actccgcccc gcgcggcgag ctgcgcgtgt cggcgtcgtt ttcgtttggc atgggcgcgc
     7261 tggtgcccat cgtgccggat ttcatggccc gctacccgga catccgcctg caactggagc
     7321 tgcgcaacga cgcggtggac ctggtggccg aaggctttga cctggccatc cgcatcggcc
     7381 cgctggagga ttcatcgtgc gcggtgcgct atctggcgga aagccgcctg gcgctgtacg
     7441 ccagcccgga ctacctggct cgcgccggca tgccggccac gctggacgaa gtggcgcgcc
     7501 ggcccacgct gaccttatcc cggctgggcc gccacggccg tttttactgg ccgctggccc
     7561 ggcaaaatgg cgaagcgcgc gagctggcca tcaccccgca actggtggcc aacgaccccg
     7621 gcgtcatcaa atttgccgcg ctggccggcc tgggcgtggc gctgctgccg gtgatcctga
     7681 tccgcccgga agtcgaatcc ggccagctgc tgccggtgct gcccgactgg gaaggcccgc
     7741 ccactgaaat cggcgcggtc taccccagcc ggcgcgggct gtcgcccaag gtgcgggtgt
     7801 ttattgattt tttgagcgag cggctggcgc atttgagcgg cagccggcgt ggctgaaacc
     7861 gcgctgctgg tatattctg
//
LOCUS       DAOPYX010000133         7876 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_11071_length_7876_cov_13.028513, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000133 DAOPYX010000000
VERSION     DAOPYX010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 7876)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7876)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7876
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11071_length_7876_cov_13.028513"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(7..432)
                     /locus_tag="PKN55_07980"
     CDS             complement(7..432)
                     /locus_tag="PKN55_07980"
                     /inference="COORDINATES: protein motif:HMM:NF040319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6088 family protein"
                     /protein_id="HNX71221.1"
                     /translation="MSRFSIKARMQRSIALRKGEVVLRADFAQLGSPSQISRALKALQ
                     AEGRIVRLGYGVYAKARPSVLSGKPIPRVTLEELAQETLRKLGAPAELGRAQQAYAQG
                     RSTQIPAHTTFNTGQRRISRKITVGISTVRYENDYKPRA"
     gene            893..3517
                     /gene="cphA"
                     /locus_tag="PKN55_07985"
     CDS             893..3517
                     /gene="cphA"
                     /locus_tag="PKN55_07985"
                     /EC_number="6.3.2.29"
                     /EC_number="6.3.2.30"
                     /inference="COORDINATES: protein motif:HMM:NF010623.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0051235 - maintenance of location [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HNX71222.1"
                     /translation="MKKKDIEFLDVLSLRGPNIWTYRPVLEAWIDIGELEDFPSNTLP
                     GFNERLSAWLPSLVEHRCSIGERGGFLQRLRDGTWMGHILEHVTLELQNLAGMQAGFG
                     KAREAGPRGVYKVAIRARNETVSRAALNTARALLMAAIEDRPFDVAAAVAELRDLVDS
                     HCLGPSTACIVDAATDRGIPSMRLNDGNLVQLGYGVNQRRIWTAETDQTSAIAEGISR
                     DKDLTKQLLGYCGVPIPEGRLVDSPEDAWDAAEDIGLPVVVKPYDGNHGRGVCANLNS
                     REEVMAAYPVARAEGSGVIVERFIFGAEHRLLVVGGKLAAAARGEEAAVVGDGRSTIV
                     ELINSQLNTDPRRGEEEEFPLTPILLEREPAARLELSRQGYDGQSIPPAGQRVLVQRN
                     GNMTCDITSLVHPDVAAAAVLAARVVGLDIAGVDIVAEDISRPLAEQRGAIVEVNAGP
                     GLLMHLKPEFGEPRPVGRAIVDSLFPAGNDGRIPVVAVGGTNGKTVVAQLVASLLRLS
                     DKRVALSCSDGLYFDQRRIGRGDGCGWEAARKCLINRSIEAAVIESDSRHILGEGLAY
                     DRCQVAVLTGIGAGDHLGDYYIEQPEQLFTVWRTLVDVVLPGGAAVLNAADPQLVEMA
                     ELCDGEVIYFARARNAVIDGHLAAGGRAVLLEDGQIVLAQGREQRQTLGVAADVPLSY
                     DGRVGFQLDNLLAALAAAWALGLSDDLMRTGVDTFDVGQAGVPGRFTVFRRGEATVVV
                     DDAHNADAMRALIEALDAFPAARRHAVCALGAPRRSQDWQAQAAVLAAGFDQLTLTTD
                     ASLTVQATAEAQAAFTAALHGGRLDGCQRQDDPRLAISQALAALTGSADLLWIQTGEG
                     PEGGLLALELARQWAGQA"
     gene            3610..6162
                     /gene="cphA"
                     /locus_tag="PKN55_07990"
     CDS             3610..6162
                     /gene="cphA"
                     /locus_tag="PKN55_07990"
                     /EC_number="6.3.2.29"
                     /EC_number="6.3.2.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012697440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0051235 - maintenance of location [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HNX71223.1"
                     /translation="MDVSRIRALRGPNLWSRHTAIEAIVSCTPAECAIDERAGFEAAL
                     RARFPAIGEIGRSLASALEAAALALQAQAGCPVTFSRTTETVEPGIYQVVVEYSEEAV
                     GRLAFDLACQLCEAAMNDAPFELDAALAQLRELDEDVRLGPSTGAIVDAAVARGVPFR
                     RLTDGSLVQFGWGSRQQRIQAAETSRTSAIAESIAQDKELTKMLLESAGVPVPNGRSV
                     SDAEDAWAAACELGGPVVVKPRDGNQGKGVAVNLSSREEIMAAYAVAVEFRDDVMVER
                     YLPGHDYRLLVIGNQLVAAARRDPPQVIGDGVHTIRELVDIVNRDPLRSDGHATSLTK
                     IRFDDIALATLARQKLHADTVPAKGQRVVLRNNANLSTGGSATDVTDDVHPEVAARAV
                     AAARMVGLDICGVDVICETVLKPLEEQGGGIVEVNAAPGLRMHLAPSFGKGRAVGEAI
                     INTMYPAQDNGRIPVVAVAGTNGKTTTVRLIAHLLGSKGLRVGMTNSDGVYIQRRRID
                     TGDCSGPRSARSVLMHPEVDAAVFETARGGVLREGLAFDRCDVAVVTNIGAGDHLGLN
                     YITTVEDLAVVKRVIVQNVAPSGMAVLNAADPSVVAMAASCPGGVTFFARDRNHPVMA
                     THRAQGHRIVYLEDGQIVAAQGEMRHRIALTEIPLTQGGRIGFQVENAMAAVAAAWAL
                     GLEWPVVHAGLASFINDAATAPGRFNTFNYQGAMVIADYGHNPDAMLALVQAIDNLPS
                     ERRSVVISGAGDRRDQDIRDQTRILGAAFDSVILYQDACQRGRADGEVLALLRQGLQD
                     APRTRHSEEIQGEFNAIDRALAQLNPGDLCLILVDQVEEALAHIAKRVAEVS"
     gene            complement(6270..6779)
                     /locus_tag="PKN55_07995"
     CDS             complement(6270..6779)
                     /locus_tag="PKN55_07995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008952095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HNX71224.1"
                     /translation="MTPFTLAGKLRRLGSLLYEALLLAALLLAAAALFQPLFAWLGQA
                     AWLHALFQLYLLAVLYFYCDWSWRRGGQTLAMKTWKIRLARADGQALDRRSAALRFAV
                     LAALLIGFPALSYLAWGRVLAPKQAGIVAALWLLLPYGWAWLDPDGQFLHDRLAKTRL
                     IMAPPRKAG"
     gene            complement(6807..7394)
                     /locus_tag="PKN55_08000"
     CDS             complement(6807..7394)
                     /locus_tag="PKN55_08000"
                     /inference="COORDINATES: protein motif:HMM:NF022748.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3106 domain-containing protein"
                     /protein_id="HNX71225.1"
                     /translation="MLVGPRRLAVSLACAGLPLGSAHAGPAVAQPPAASAVAAPVAKA
                     APVGPLWHELTPQQRQILTPVASEWNGFSGPKKQRLLNVAKRYPDMSPAEQLKIQARL
                     PHWTELPQTTRDEARNNMKKLLQLPPDERKRTEEKLRAKLAPPPAVAPLAPTPVAPAP
                     AAAPAAAPTAAAPSQTIAAPASAPIPPAEAAKPLQ"
     gene            complement(7410..7763)
                     /locus_tag="PKN55_08005"
     CDS             complement(7410..7763)
                     /locus_tag="PKN55_08005"
                     /inference="COORDINATES: protein motif:HMM:NF023699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3619 family protein"
                     /protein_id="HNX71226.1"
                     /translation="MKHDALQRLLVEHLNSRPVPPHAAARLARARDAALARHMQRQHQ
                     PARLPEWLGRWLTAHNAAARRSAWAMAMVTALSVGYLGWTEWSAPSDADVDIALLTSD
                     LPVEAYLHPNFTRDL"
BASE COUNT         1334 a         2590 c         2714 g         1238 t
ORIGIN      
        1 atccaatcat gctcttggtt tgtaatcgtt ttcataacgc accgtgctga tgcctactgt
       61 gatttttcgg gaaatgcgcc gctgcccggt gttgaacgtg gtgtgcgccg gaatctgcgt
      121 gctcctgccc tgggcgtagg cttgttgcgc ccggcccagt tcggcgggcg cgcccagttt
      181 gcgcagggtt tcctgcgcca gttcttccaa ggtgacgcgc ggaatgggct tgccggacaa
      241 aacactcggg cgcgccttgg catagacgcc ataacccagc cggacaattc tgccctcggc
      301 ctgtagcgcc ttgagcgccc ggctgatctg gctggggctg cccagttggg caaagtcggc
      361 gcgcaacacc acctcgccct tacgcagggc aatcgagcgc tgcatgcgcg ccttgatgct
      421 gaaacgagac ataaactcct ccgccacggt cgtcaaacac cagcattgac ctactctcac
      481 ctaggccaat attggcaaat ttatccaata aaaacaatca caaaatacag cacacacagc
      541 gcattgacac ttggcgccgc tcacacaagc tcagcgctgc gatcctggct aggcgcgcag
      601 ccgcaaacag tgcaagcact accgccaggc cgcacaacaa cgccaggagg ggtttgtgag
      661 cggcgctaac tcacgtcatg cgcgggcccg ccgtcacgcc gccacgacaa gggccgtggt
      721 gggcgccgtg caatttttgt aaagctgggt aaacctgaca gccgctgctg cgttaaagat
      781 gtgttatggc cttgcgtccg ggtgcggcaa gtgtctgacc cggtgcgccg gcccaatggc
      841 ccgcgtgtgt gaattttgcg tcccgacccc ctgttgtgtt tctgccaagc ctatgaaaaa
      901 gaaagacatt gaatttctcg atgtcctgtc cctgcgcgga cctaacatct ggacttaccg
      961 cccggtgctg gaagcctgga ttgatatcgg tgagctggag gattttcctt ccaacacctt
     1021 gcccggcttt aacgagcgcc tgagcgcctg gttgccgtcg ctggttgaac atcggtgcag
     1081 cattggcgag cgcggcggct ttttgcagcg gctgcgcgat ggcacctgga tgggccacat
     1141 ccttgagcac gtcaccctgg agctgcaaaa cctggccggc atgcaggcgg gctttggcaa
     1201 ggcgcgcgaa gccgggccgc gcggggtgta caaggtggcg atccgcgccc gcaacgaaac
     1261 cgtgtcgcgc gctgcgctga acaccgcgcg cgcgctgctg atggccgcca tcgaagaccg
     1321 cccgtttgac gtggctgcgg cggtggccga gctgcgcgat ctggtggatt cgcactgtct
     1381 ggggccgagc acggcgtgca ttgtcgatgc cgccaccgac cgcggcatcc cgtcgatgcg
     1441 gctgaacgac ggcaatctgg tgcaattggg ctatggcgtg aaccagcgcc gcatctggac
     1501 cgccgaaacc gaccagacca gcgcgattgc cgaggggatt tcccgcgaca aagacctcac
     1561 caagcagctg ctgggctatt gcggcgtgcc gattcctgaa ggccggctgg tggacagccc
     1621 ggaagacgcc tgggacgccg ccgaagacat cggcctgccg gtggtggtca agccgtatga
     1681 cggcaaccac ggccgtggcg tgtgcgccaa cctgaattcg cgcgaagaag tgatggccgc
     1741 ctacccggtg gcgcgcgccg aaggcagcgg ggtgattgtc gaacgcttta tttttggcgc
     1801 tgaacaccgt ctgctggtgg tcggcggcaa attggccgcc gccgcgcgcg gcgaagaagc
     1861 cgccgtggtg ggcgatggcc gctcgaccat tgtcgagctg atcaacagcc agctcaacac
     1921 cgacccgcgt cgcggcgaag aagaagaatt cccgctcacc ccgattctgc tggagcgcga
     1981 acccgccgcc cggctggagc tgtcgcgcca gggctacgat ggccagtcca tcccgccggc
     2041 cggccagcgc gtgctggtgc agcgcaacgg caatatgacc tgcgacatca ccagcctggt
     2101 gcacccggac gtggccgccg ccgccgtgct ggcggcgcgc gtggtggggc tggacatcgc
     2161 cggggtggat attgtcgcgg aagacatctc ccgcccgctg gcagaacagc gcggggcgat
     2221 tgtggaagtc aacgccggcc ccggcctgtt gatgcacctg aaacccgaat ttggcgaacc
     2281 gcgcccggtg ggccgtgcca ttgtcgacag cctgttcccg gcgggcaacg acggccgcat
     2341 cccggtggtg gcggtgggcg gcaccaatgg caaaaccgtg gtggcgcagc tggtggccag
     2401 cctgctgcgg ctgagcgaca agcgcgtggc gctgtcgtgc agcgacggcc tgtattttga
     2461 ccagcgccgc atcggccgtg gcgatggctg cggctgggaa gccgcgcgca aatgcctgat
     2521 caaccgcagc atcgaagctg cggtgatcga gagcgacagc cgccatatct tgggcgaagg
     2581 tctggcctac gaccgctgcc aggtggcggt gctgaccggc attggcgctg gcgaccatct
     2641 gggcgactac tacatcgaac agccggaaca gctgttcacc gtctggcgca ccctggtgga
     2701 cgtggtgctg ccgggcggcg ccgccgtgct gaacgccgcc gacccgcaat tggtggagat
     2761 ggccgagctg tgcgatggcg aggtgatcta ttttgcccgc gcccgcaatg cggtgatcga
     2821 cggccatctg gccgctggcg ggcgggccgt gctgctggaa gacggccaga ttgtgctggc
     2881 gcagggccgc gagcagcgcc agacgctggg cgtggccgcc gatgtgccgc tgagctacga
     2941 cggccgcgtc ggctttcagc tggacaacct gctggccgcg ctggccgccg cctgggcgct
     3001 gggcctgagc gacgacctga tgcgcactgg cgtagacact tttgacgttg gtcaggccgg
     3061 cgtgccgggc cgctttaccg tgttccgtcg cggcgaagcc accgtggtgg tggacgacgc
     3121 ccacaacgcc gacgccatgc gcgcgctgat tgaggcgctg gacgcctttc cggcggcgcg
     3181 ccggcatgcg gtgtgcgcgc tgggcgcgcc gcgccgcagc caggattggc aagcccaggc
     3241 cgccgtgctg gcggcgggct ttgaccagct gacgctgacc accgacgcca gcctgaccgt
     3301 gcaagcaacg gccgaggcgc aagcggcgtt caccgccgcg ctgcacggcg gccggctgga
     3361 cggctgtcag cgtcaggacg atccgcgtct ggccatcagc caggcgctgg cggcgctgac
     3421 cggcagtgcc gacctgctgt ggattcaaac cggagaaggc cccgagggag gcctgctggc
     3481 gctggagctg gcgcgccagt gggcgggcca ggcctgaggc atcagaccga gacacgacac
     3541 cagacaaaca ataacggttg cccgccgcat gcggcgggac caccgggcat caaggaacat
     3601 tctcaagcta tggacgtttc ccgcatccgc gccctgcgcg gccccaatct gtggagccga
     3661 cacaccgcca tcgaagccat tgtgtcgtgc accccggcag aatgcgccat tgacgagcgc
     3721 gccggctttg aagccgcgct gcgcgcgcgc tttccggcca ttggcgaaat cggccgctcg
     3781 ctggccagcg cgctggaggc cgccgccctg gcgctgcaag cgcaagccgg ctgcccggtg
     3841 actttcagcc gcaccacgga aaccgtcgaa cccggcatct atcaagtggt ggtggaatac
     3901 agcgaagaag ccgtcggccg cctggcgttt gacctggcct gccagctgtg cgaagcggcg
     3961 atgaacgacg cgccgtttga gctggacgcc gcgctggccc agctgcgcga gctggacgaa
     4021 gacgtgcgcc tggggccgag cactggcgcg attgtcgacg ccgccgtggc gcgcggcgtt
     4081 cccttccgcc ggctgaccga cggcagcctg gtgcaatttg gctggggcag ccgccagcag
     4141 cgcattcagg ccgccgaaac cagccgcacc agcgccattg ccgaatccat cgcccaggat
     4201 aaagaactca ccaagatgct gctggagtcc gcgggcgtgc cggtgcccaa tggccgctcg
     4261 gtcagcgacg ccgaagacgc ctgggccgcc gcctgcgagc tgggcgggcc ggtggtggtc
     4321 aagccgcgcg acggcaacca gggcaagggc gtggcggtga atctgtccag ccgcgaagaa
     4381 atcatggccg cctacgccgt ggcggtggag tttcgcgatg atgtgatggt cgagcgctac
     4441 ctgcccggcc acgattaccg cctgctggtg atcggcaacc aactggtggc cgccgcccgc
     4501 cgcgacccgc cgcaggtgat tggcgatggc gtgcacacca tccgcgagct ggtggacatc
     4561 gtcaaccgcg acccgctgcg cagcgacggc cacgccacct cgctgaccaa aatccgcttt
     4621 gacgacatcg ccctggccac cctggcccgg caaaaactgc acgccgacac cgtgccggcc
     4681 aagggccagc gcgtggtgtt gcgcaacaac gccaacctga gcacgggcgg cagcgccacc
     4741 gacgtcaccg acgacgtgca cccggaagtc gccgcccgcg cggtggccgc cgcgcgcatg
     4801 gtcggcctgg acatctgcgg cgtggacgtg atttgcgaaa ccgtgctgaa accgctggaa
     4861 gaacaaggcg gcggcattgt cgaagtcaac gccgcccccg gcctgcgcat gcacctggcg
     4921 ccgtcgttcg gcaagggccg cgccgtcggc gaagccatca tcaacaccat gtacccggcg
     4981 caagacaacg gccgcatccc ggtggtggcg gtggccggca ccaatggcaa aaccaccacc
     5041 gtgcgcctga ttgcccatct cttgggcagc aagggcctgc gcgtgggcat gaccaactcc
     5101 gacggcgtgt acattcagcg ccgccgcatc gacaccggcg attgctctgg cccgcgcagc
     5161 gcgcgcagcg tgctgatgca cccggaagtg gacgccgccg tattcgaaac cgcccgtggc
     5221 ggcgtgctgc gcgaaggcct ggcctttgac cgctgcgacg tggccgtggt caccaatatc
     5281 ggcgctggcg accacctggg cctgaactac atcaccacgg tggaagacct ggccgtggtc
     5341 aagcgggtga tcgtgcaaaa cgtcgccccc agcggcatgg ccgtgctcaa cgccgccgac
     5401 cccagcgtgg tggccatggc cgccagctgc cccggcggcg tgaccttctt tgcccgcgac
     5461 cgcaaccacc cggtgatggc cacccaccgc gcccaaggcc accgcattgt ctatctggaa
     5521 gacggccaaa tcgtcgccgc ccagggcgaa atgcgccacc gcatcgccct gaccgaaatc
     5581 ccgctcaccc aaggcggacg cattggtttt caggtggaaa acgccatggc cgccgtcgcc
     5641 gccgcctggg cgctgggcct ggaatggcca gtggtgcacg ccggcctggc ctcgttcatc
     5701 aacgacgccg ccaccgcgcc gggccgcttc aacaccttta actaccaggg cgcgatggtc
     5761 atcgccgact acggccacaa cccggacgcc atgctggccc tggtgcaagc catcgacaac
     5821 ctgcccagcg aacgccgcag cgtggtgatc agcggcgccg gcgaccgccg cgaccaagac
     5881 atccgcgacc aaacccgcat cctcggcgcc gcgtttgatt cggtgatcct ctaccaggac
     5941 gcctgccaac gcggccgcgc cgacggcgaa gtgctcgccc tgctgcgcca aggcctgcaa
     6001 gacgccccgc gcacccgcca cagcgaagaa atccagggcg aattcaacgc catcgaccgc
     6061 gcgctggccc aactgaatcc gggtgatttg tgcctgatcc tggtcgatca ggtggaagaa
     6121 gccctggcgc atattgccaa gcgggtggcg gaggtgagtt gaggtaggtg agataacggg
     6181 cggcaaggta aaacgcgctg cccgtgatag ccagatggaa aaatacaggc cacgccggcg
     6241 acggcgtggc ctgtttcatg accgcgcaat caccccgcct tgcgcggcgg ggccatgatc
     6301 aaccgggttt tggccaggcg gtcgtgcagg aattggccgt ccgggtcgag ccaggcccag
     6361 ccgtagggca gcagcagcca cagggcggcg acgatgccgg cttgtttggg cgccagcacg
     6421 cggccccagg ccaggtagga cagcgccgga aagccaatca gcagggcggc cagcacggca
     6481 aagcgcaggg cggcgctgcg gcggtccagc gcttggccgt cggcgcgggc caggcggatt
     6541 ttccaggttt tcatcgccag ggtttgcccg ccgcgccgcc aggaccagtc gcagtaaaaa
     6601 tacagcacgg ccaacagata aagctggaag agcgcatgca gccaggccgc ttggcccagc
     6661 caggcaaaca gcggttgaaa cagcgcggcg gcggccagca gcagggcggc cagcagcagg
     6721 gcttcgtaga gcaggctgcc cagccggcgc agtttgccgg caagcgtgaa tggggtcatg
     6781 accgagtcgg gcgaagtcga aacggcttat tgtagcggtt ttgccgcttc ggctgggggg
     6841 atgggcgcgc tggccggggc ggcgatggtt tgggatggcg ccgcggccgt gggggctgcg
     6901 gcgggcgcag ctgccggcgc cggggcgacg ggggttggcg ccagcggcgc gacggcgggc
     6961 ggaggggcaa gcttggcgcg cagtttttct tcggtgcgct tgcgttcatc tggcggcagc
     7021 tgcaacagtt ttttcatatt gttgcgcgct tcgtcgcggg tggtttgcgg cagctcggtc
     7081 cagtgcggca gccgcgcctg gattttcagt tgctcggccg ggctcatgtc ggggtagcgc
     7141 ttggccacat tgagcaggcg ctgttttttg ggcccggaaa acccgttcca ttcgctggcc
     7201 accggggtga gaatttggcg ttgctggggg gtgagctcat gccacagcgg ccccaccggt
     7261 gcagctttgg cgacgggcgc cgccacggcg ctggcggccg gcggctgcgc caccgccggg
     7321 ccggcgtgcg cgctgcccag cggcaagccc gcgcacgcca ggctgacggc aagacggcgg
     7381 gggccaacaa gcaagcgcca gcgcatggct tacagatctc gggtgaagtt ggggtgcaga
     7441 taggcttcca ccggcaaatc gctggtgagc agggcgatat ccacatcggc gtcgctgggc
     7501 gcgctccatt cggtccagcc cagatagcca accgacagtg cggtgaccat ggccatggcc
     7561 caggcgctgc gccgggcggc ggcgttgtgc gcggtcagcc agcggcccag ccattccggc
     7621 aggcgcgcag gctgatgctg gcgctgcatg tggcgggcca gcgcggcgtc gcgcgcgcgc
     7681 gccagccggg cggcggcgtg cggcggcacg gggcgcgaat tcaggtgttc aaccagtagg
     7741 cgttgaagag cgtcgtgttt catgatgttc acaatgtcac accgtgccgg gtcaaccagg
     7801 ccgccagggc gtgggtggcg cgggagcagt gggttttcac gctgccttgc gagcagccca
     7861 tggcggcggc ggtgtc
//
LOCUS       DAOPYX010000134         7821 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_11184_length_7821_cov_10.955061, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000134 DAOPYX010000000
VERSION     DAOPYX010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 7821)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7821)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7821
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11184_length_7821_cov_10.955061"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            313..2538
                     /locus_tag="PKN55_08010"
     CDS             313..2538
                     /locus_tag="PKN55_08010"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012698264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HNX71227.1"
                     /translation="MNSSAKPLPHKASLPASATDVSATPPVIVQVAFDVPLDRVFDYL
                     APRPLPAGVRVLAQFGPRKLVGVVVGQVAASPHQLRPLLAVLDELPPLPDDWLALTRF
                     AARYYHYPLGQTLFTALPTALRNPPRRALAMPPVYALSEAGAAWQPPARAARQLALWA
                     RLRQGPLALDDEARALHPQAGKLLREWLAAGWLAQVTPAQAAMTVSPGPALTDEQREV
                     VDGLSWAGFSATLLDGITGSGKTEVYLQWIAQALAAGRQALVLVPEINLTPQLEARFR
                     ARFPHTPTVCLHSNLADGARAQAWLAAWEGRARLVIGTRLAVFTPLPELGLIVVDEEH
                     DGSFKQQDGLRYHARDLAVWRAQQAGAPVVLGSATPSLESVAAVQAGRYRHARLTRRA
                     SAAALPQIHLLDIRRQLLDEGLSAAAWQALADGLAAGETSLVFINRRGWAPVLACVDC
                     GWLSSCPHCAARLVLHWHDRRLQCHHCGHHEAIPHACPSCGNPDLKPLGQGTQRIEAA
                     LRRQFPQARIARIDRDTVSRRDAWAEIDRQVRAGELDILVGTQMLAKGHDFPSLSRVV
                     ALNADGGLYSADFRAEERLFALLTQVAGRAGRGALPGQVWVQTQFPDHPLYLALREHD
                     VAGFAQRLLEERASAGYPPAVFQALLRADAPELDTAMAFLREVRQYAACHGDVLSLGP
                     APASMARLAGRERAQLLLQAERRAPLHAALSALCAGLDDWARPFGSALRWSLDVDPQE
                     L"
     assembly_gap    2566..2665
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2705..4090
                     /gene="dacB"
                     /locus_tag="PKN55_08015"
     CDS             2705..4090
                     /gene="dacB"
                     /locus_tag="PKN55_08015"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011134680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HNX71228.1"
                     /translation="MMLRPLLAACCLLLPSLAHALNLGAIRPDEVAIWMAPQDGGPAI
                     VQHRADAPMNPASTMKLLTTLSALELLAPDYRWRTELLADARPRGDTLDGPLYWKGGG
                     DPHFDRNDLAALARDLRGRGIRHLAGDVVLDKQRFARTGSADDFASDSDKAFAVPPDA
                     LLAHLKVVWTTLFVQPDGVRVALDPPLDGLKVDNRLTVSQQANCEGGVKRYASLSAQG
                     DTLRLEGALPAGCDGGKLFAPVWEHDAYQAAMFAALWREAGGSLAGQVRRGLTPANAV
                     TLASVSSPTLAEVVRDINKYSNNTMARQLFLTLGAEFAQEGDVLRDGEAAVRRWATQR
                     KLALPGLQLENGSGLSRRERISAAGLGALLQAGANSPYAPEFMASLPIAARDGTLRRR
                     FVGQPVEARLKTGSLNDVRALAGYVKAVDGRRYALVVLINSPRASELTPAMDALVAGL
                     IRHPGRLEAEP"
     gene            4087..>4664
                     /locus_tag="PKN55_08020"
     CDS             4087..>4664
                     /locus_tag="PKN55_08020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX71229.1"
                     /translation="MSRLVALLTLSLALAAPAGASTPVPSAPTQRLNWQIQPGRSLAL
                     IALDSSEASLIERFGAFNVQPAQIKLDAGEPYPASVLFPRDANKRIALVWRDSVKRSG
                     VAVAVVRGERSFWTLPGGLGLGSTVAELEKRNGRPLLLTGLGPARTGQVLGWNNGALA
                     SSLARVSLLLGDYDYALLSAPARQQVLVGPYLS"
     assembly_gap    4665..4764
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4765..5426)
                     /locus_tag="PKN55_08025"
     CDS             complement(<4765..5426)
                     /locus_tag="PKN55_08025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX71230.1"
                     /translation="MLISPYASSSQRLYDKISQATSTPASALGAPTVASRDSATVTLS
                     VEARAIQALLQNKPDEVNASSAAGFNDFLARFGEQTHAQGKAAPMLAELPESADPARL
                     ALAHQAVNFVLSKVGQPSAYAEQSSQNPFAELGRKSLSRIAFDDSGTFTSAERSAAHQ
                     EIQAREEDFTKAVKAQQESLTRRDKSYSAFWQQLTQTQAQATQLAEMTETERAWRGWG
                     TL"
     gene            complement(5551..>7821)
                     /locus_tag="PKN55_08030"
     CDS             complement(5551..>7821)
                     /locus_tag="PKN55_08030"
                     /inference="COORDINATES: protein motif:HMM:TIGR00361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HNX71231.1"
                     /translation="LMPYLLLFAAGVTLAQCCLAPPSWMAALACAVLLGCGPWRRHGV
                     WLLALIVGFAWSAWRIEQRLASAWPAQWEGRPVMVTLRVVGLPRQDAMATRLDAVLEH
                     VELSPPLANWPARVGVSDYQRGDWPAGSRWRAQLTLRRWRATSNPHTFAAEAWLMEQG
                     RLASGSVRPGAQRLADSQHPQAWLDRWRAAAVARVTRAAGEHTYSGVLTALITGEQGY
                     IPPEHWRLFAQTGLTHLVSVSGVHLTLLAGLVASVTIWLWRRQTRWRAPPRLAGAVAG
                     LLAASAYGLLAGWSLPTQRSVYMLWALALALCGRYPLGVGRAWALALCAVLTLDPFAA
                     LSLGFWLSFFAVGLMIWVGAGRVGALPWWRQWGRAQWAASLAMAVPLLASFGELPWLS
                     PLSNLYAIPLIGSVLTPAALLASALPTDLGVAWVAQALDWAMWPLEHLAGAPMWAQAA
                     PTTLAIALASLGVAVLSLPAGVAGRALGLALCLPLFCPPERAPPPGQAQITVLDVGQG
                     LAVVVRTAQHTLVYDTGARVGASDMGARVIAPFLAGEGVARLDGLVVSHSDMDHAGGA
                     ASLWGRFAPGWLLAGEPERLSKERPPVAPQPCRAGQHWQWDGVRFDVLYPIHGGASDN
                     HASCVLRVWAQGQALLLTGDAELADEDAMLAHWQPDALAADVLVVGHHGSRTSSGQAF
                     LQAVRPREAVISAGYRNRYHHPHPAVWTRLADAGARRWRTDRDGALRLTLGAAADIQP
                     EGFRTARPHWWQSSWP"
BASE COUNT         1141 a         2679 c         2618 g         1183 t
ORIGIN      
        1 caatccgcgc tatcatcccc gcgtagtcac cagccacatg cctgagtctg gccgattttc
       61 atctgctgat gtctcttttt tccttgacaa gccgggcttc gcttgatcct gatgggcgag
      121 ttatacacac cagcgtcagg cggcaaaagt gggattcaat gtgactgtca cgagcagctg
      181 tatttgtcta aattattgtt ttataagcaa gaatttctct gatgattttt tggtcaatcc
      241 aacagaagcc ttgcccgtcg ggcatccgtc cggtttgtcc gccgattgtc cacatcgtta
      301 tccacagctt gtgtgaatag ttccgccaag cccttgccgc acaaggcttc tttgcctgcg
      361 tcggcgaccg atgtcagcgc gacgccgccg gtgatcgtgc aggtggcgtt tgatgtgccg
      421 ctggaccggg tgttcgacta tctggccccg cgcccgctgc cggcgggcgt gcgcgtgctg
      481 gcgcagtttg gcccgcgcaa attggtgggg gtggtggttg ggcaggtggc ggcgtcgccg
      541 catcagctgc gccccttgct ggcggtgctg gacgagctgc cgccgctgcc ggacgactgg
      601 ctggcgctca cccgctttgc cgcgcgctac taccattatc cgctggggca aaccctgttc
      661 accgccctgc ccaccgcgct gcgcaacccg ccgcgccgcg cgctggccat gccgcccgtg
      721 tatgcgctga gcgaagccgg cgccgcctgg cagccgccag cgcgggcggc gcgccagttg
      781 gcgctgtggg cgcggctgcg gcaagggccg ctggcgctgg atgacgaggc gcgcgcgctg
      841 catccgcagg ccggcaagct gctgcgcgaa tggctggccg ccggctggct ggcgcaggtg
      901 acgccagcgc aggcggccat gacggtgagc cccggcccgg cgctgaccga cgaacagcgc
      961 gaggtggtgg atgggctgag ctgggcgggg ttttccgcca ccttgctgga cggcatcacc
     1021 ggcagcggca aaaccgaagt gtatttgcag tggattgccc aggcgctggc cgccggccgc
     1081 caggcgctgg tgctggtgcc ggaaatcaac ctcaccccgc agctggaggc gcgctttcgc
     1141 gcgcgttttc cgcacacgcc aacggtttgc ctgcacagca atctggccga cggcgcgcgc
     1201 gcgcaagcct ggctggccgc ctgggaaggc cgcgcccggc tggtgattgg cacccggctg
     1261 gcggtgttca ccccgctgcc cgagctgggg ctgattgtgg tggacgaaga gcacgatggc
     1321 tcgttcaagc agcaagacgg cctgcgctac cacgcccgcg atctggcggt gtggcgcgcg
     1381 caacaggccg gcgcgccggt ggtgctgggc tcggccacgc cctcgctgga aagcgtggcg
     1441 gcggtgcagg ccggccgcta tcgccacgcc cggctgaccc gccgcgccag cgccgccgcg
     1501 ctgccgcaga ttcatttgct ggatattcgc cgccagttgc tggacgaagg cttgagcgcc
     1561 gccgcctggc aggcgctggc cgacggcctg gccgcaggcg aaaccagcct ggtgttcatc
     1621 aaccggcgcg gctgggcgcc ggtgctggcc tgtgtggact gcggctggct gtcgtcctgc
     1681 ccgcattgcg cggcgcggct ggtgctgcac tggcacgacc gccgcctgca atgccaccac
     1741 tgcggccacc acgaagccat cccgcacgcc tgccccagct gcggcaaccc cgacctgaaa
     1801 ccgctggggc aaggcaccca gcgcatcgaa gccgccttgc gccggcagtt tccgcaggcg
     1861 cgcattgccc gcatcgaccg cgacaccgtc agccggcgcg acgcctgggc cgagatcgac
     1921 cgccaggtgc gcgcgggcga gctggatatc ctggtcggca cgcaaatgct ggccaagggc
     1981 catgatttcc ccagcctgtc gcgggtggtg gcgctgaacg ccgatggcgg gctgtatagc
     2041 gcggattttc gggcggaaga acgcctgttt gccctgctca cgcaagtggc cggccgcgcc
     2101 ggccgtggcg cgctgcccgg ccaggtgtgg gtgcagacgc agtttcctga tcacccgctg
     2161 tatctggccc tgcgtgagca cgatgtggcc ggctttgccc agcgcctgct ggaagagcgc
     2221 gccagcgccg gctatccgcc tgcggtgttt caggcgctgt tgcgcgccga cgcgcccgag
     2281 ctggacaccg ccatggcatt tttgcgcgag gtgcgccaat atgccgcgtg tcatggcgat
     2341 gtgctcagcc tgggcccggc gccagccagc atggcgcggc tggccgggcg cgagcgcgcg
     2401 cagctgctgt tgcaggccga gcgccgcgcg ccgctgcacg ccgcgctgtc ggcgctgtgc
     2461 gccggcctgg acgactgggc gcggccgttt ggctcggcgc tgcgctggtc gctggatgtg
     2521 gatccacaag agctatgagg cgcaccgcct gatttttacc cacttnnnnn nnnnnnnnnn
     2581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2641 nnnnnnnnnn nnnnnnnnnn nnnnngcggg gttttgtgag cgacgcttaa ccttgccctg
     2701 acttatgatg ctccgtcccc tgcttgctgc ctgttgcctg ctcttgccca gcctggccca
     2761 cgccctcaac ctgggcgcca tccgccctga cgaagtcgcc atctggatgg ccccgcaaga
     2821 cggcgggccg gcgattgtcc agcatcgcgc cgacgcgccg atgaacccgg cctccaccat
     2881 gaagctgctg accacgctgt cggcgctgga gctgctggcc cccgactacc gctggcgcac
     2941 cgaactgctg gccgacgccc gcccgcgcgg cgacaccctg gacggcccct tgtactggaa
     3001 aggcggcggc gacccgcact ttgaccgcaa cgacctggcg gcgctggcgc gcgacctgcg
     3061 cgggcgcggc atccgccatc tggccggcga cgtggtgctg gataaacagc gctttgcccg
     3121 caccggcagc gccgacgact ttgccagcga cagcgacaaa gcctttgccg tgccgccgga
     3181 tgcgctgttg gcgcacctga aagtggtgtg gaccacgctg tttgtgcagc cggacggcgt
     3241 gcgcgtggcg ctggatccgc cgctggatgg actgaaagtg gacaaccggc tgaccgtcag
     3301 ccagcaggcc aattgcgaag gcggcgtcaa acgctacgcc agcctgagcg cacaaggcga
     3361 caccctgcgg ctggaaggcg cgctgccggc cggctgcgac ggcggcaagc tgtttgcccc
     3421 ggtgtgggag cacgacgcct atcaggcggc gatgtttgcc gcgctgtggc gcgaggccgg
     3481 cggcagcctg gccggccagg tgcgccgggg ccttaccccg gccaatgcgg tgacgctggc
     3541 cagcgtcagc tcgcccaccc tggccgaagt ggtgcgcgac atcaacaaat acagcaataa
     3601 caccatggcc cgccagctgt ttctcaccct gggcgccgag tttgcgcaag aaggcgatgt
     3661 gctgcgcgac ggcgaagccg ccgtgcgccg ctgggccacc cagcgcaagt tggccctgcc
     3721 cggcttgcag ctggaaaacg gctcgggcct gtcgcggcgc gagcgcatca gcgccgctgg
     3781 cctgggcgcg ctgctgcaag ccggcgccaa ctcgccgtat gcgccagaat tcatggcctc
     3841 gctgccgatt gccgcccgcg acggcaccct gcgccggcgc tttgtcggcc agccggtgga
     3901 agcccgactc aaaaccggct cgctgaacga tgtgcgcgcg ctggccggct atgtcaaagc
     3961 cgtcgatggc cgccgctatg cgctggtggt gctgatcaac agcccgcgcg ccagcgagct
     4021 gaccccggcc atggatgcgc tggtggccgg gctgatccgc caccccggcc ggctggaggc
     4081 cgagccatga gccggctggt cgccctgctg accctcagcc tggcgctggc tgcgccagcg
     4141 ggggcaagca cgcccgtgcc cagcgcgccc acccagcggc tgaactggca aatccagccc
     4201 ggccgcagcc tggcgctgat tgcgctggac agcagcgaag ccagcctgat cgagcgcttt
     4261 ggcgcattta atgtgcaacc cgcccagatc aagctcgacg ctggcgagcc ctacccggcc
     4321 agcgtgctgt ttccgcgcga cgccaataaa cgcatcgccc tggtctggcg cgacagcgtc
     4381 aagcgcagcg gcgtggcggt ggcggtggtg cgcggcgagc gctcgttctg gacattgccc
     4441 ggcggtctgg gcctgggcag cacggtggcc gagctggaaa aacgcaacgg ccgcccgctg
     4501 ctgctcaccg gcctggggcc ggcacgcacc ggtcaggtgc tgggctggaa caacggcgca
     4561 ctggccagca gcctggcgcg ggtgagcctg ctgctgggcg actacgatta cgccctgctc
     4621 agcgccccgg cccgccagca agtgttggtt gggccgtatc tgtcnnnnnn nnnnnnnnnn
     4681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4741 nnnnnnnnnn nnnnnnnnnn nnnntccagc gtgccccaac cgcgccaggc gcgttcggtt
     4801 tcggtcattt ccgccagctg tgtggcttgc gcctgcgtct gcgtcagctg ttgccagaac
     4861 gccgaatagc ttttgtcgcg gcgagtcagc gactcctgct gcgccttgac cgccttggtg
     4921 aagtcttctt cacgggcctg aatttcttga tgggcggcgc tgcgctcggc gctggtgaat
     4981 gtgccggaat catcaaaggc aatgcgcgac aggcttttgc gccccagttc ggcaaatggg
     5041 ttttggctgg attgttcggc gtaagcagag ggttgcccca ccttgctcag tacaaaattc
     5101 accgcttggt gggccagcgc cagccgggcc ggatcggcac tttctggcag ttccgccagc
     5161 attggcgctg ccttgccctg tgcgtgtgtt tgctcgccaa aacgggccag aaaatcgtta
     5221 aaaccggcag cgctgctggc gttgacttca tcaggcttgt tttgcagcaa agcctgaatg
     5281 gcgcgcgctt ctaccgacag cgtgacggtg gcgctgtctc tggaagccac cgtgggggcg
     5341 cccaatgcac tggccggcgt gctggtggcc tggctgattt tgtcatacag ccgttggctg
     5401 ctgctggcat agggggagat caacatcggg gcgtcctgtg cagagaatat gtcgtctggc
     5461 caagcgttgg ccaaatcggg cctattatcg ctgctgctga acgcgctcac aatccgtgga
     5521 aataccgcct ggcgtggtca tgccaggcgc tcacggccag ctgctttgcc accagtgcgg
     5581 ccgcgcagta cgaaaccctt ctggctggat gtccgccgcc gcacccaggg tgaggcgcag
     5641 cgcgccgtcg cggtcggtgc gccagcggcg ggcgccggcg tcggccagcc gcgtccatac
     5701 tgccgggtga gggtggtgat agcggttgcg atagccggcg ctgattaccg cttcgcgcgg
     5761 gcgcaccgcc tgtaaaaacg cctggccgga cgaggtgcgg ctgccgtggt ggcccaccac
     5821 cagcacatcg gcggccaggg cgtcgggctg ccagtgcgcc agcatggcgt cttcgtcggc
     5881 cagttcggcg tcgccggtca gcagcagggc ttgcccctgc gcccacaccc gcagcacgca
     5941 gctggcgtgg ttgtcgcttg cgccgccgtg tatcgggtag agcacatcaa agcgcacgcc
     6001 gtcccattgc cagtgctgac cagcgcggca gggctgcggg gcgacgggcg ggcgttcttt
     6061 gctcagccgc tccggctcgc cggccagcag ccagccgggg gcaaagcgcc cccacagcga
     6121 ggcggcgccg ccggcgtggt ccatatcgct gtgcgacacc accagaccat ccagccgcgc
     6181 cacgccttcg ccggccaaaa acggcgcaat cacccgcgcg cccatatcgc tggcgccgac
     6241 ccgcgcgcca gtgtcgtaca ccagggtgtg ctgcgcggtg cgcaccacca ccgccaggcc
     6301 ctggccgacg tccagcacgg tgatctgcgc ctggccgggc ggcggcgcgc gttcgggcgg
     6361 gcaaaacaat ggcaggcaca gcgccagccc cagcgcgcgc ccggccaccc cggccggcag
     6421 gctgagcacc gccacgccta agctcgccag cgcaatggcc agcgtggtgg gcgcggcctg
     6481 cgcccacatc ggcgcgccgg ccaggtgctc cagcggccac atcgcccagt ccagcgcctg
     6541 cgccacccag gccacgccca gatcagtggg cagcgcgctg gccagcagcg cagcaggggt
     6601 gagcacgctg ccaatcagcg gaatggcgta gagattgctc agcggcgaca gccagggcag
     6661 ttcgccaaag ctggccaaca gcggcacggc catggccagg ctggccgccc actgcgcgcg
     6721 gccccattgc cgccaccacg gcagcgcgcc cacccgcccg gcgcccaccc agatcatcag
     6781 ccccacggcg aaaaacgaca gccagaagcc cagcgacaag gcggcaaaag ggtccagcgt
     6841 cagcaccgcg cacagcgcca gcgcccaggc gcggccgacg cccagcggat agcggccgca
     6901 caaggccaag gccagcgccc acagcatgta tacgctgcgc tgggtgggca gcgaccagcc
     6961 ggccaacaga ccataggcgc tggcggccaa tagcccggcc accgcgccgg ccagccgggg
     7021 cggcgcgcgc cagcgggttt gccgccgcca cagccagatg gtgacgctgg ccaccaggcc
     7081 ggccagcaag gtcagatgca cgccggagac gctgaccaga tgggtcagcc cggtctgggc
     7141 gaacaagcgc cagtgttcgg gcgggatata gccctgctcg ccggtaatca gcgcggtgag
     7201 cacgccgctg taagtgtgct cgcccgccgc gcgcgtcacc cgcgccaccg ctgctgcccg
     7261 ccagcggtcc agccaggctt gcgggtgctg gctgtcggcc aagcgctgcg cgccggggcg
     7321 cacgctgccc gaggccagcc ggccttgctc catcagccag gcttcggcgg caaaggtgtg
     7381 cggattgctg gtggcgcgcc agcggcgcag ggtgagttgg gcgcgccagc ggctgccggc
     7441 cggccagtcg ccgcgttgat agtcggacac gcccacccgc gccggccagt tggccagcgg
     7501 cggcgacagt tccacgtgtt ccagcacggc gtccagccgg gtggccatgg cgtcctggcg
     7561 cggcaggccc accacgcgca gggtgaccat caccggccgg ccctcccact gcgccggcca
     7621 ggcgctggcc aggcgttgct cgatgcgcca ggccgaccag gcaaacccca caatcagcgc
     7681 cagcagccac acgccatgcc gccgccacgg cccgcagccc agcaacaccg cgcacgccag
     7741 cgccgccatc cacgagggcg gagccaagca gcattgcgcc agcgtcacgc cagcggcaaa
     7801 caacaataaa tagggcatga g
//
LOCUS       DAOPYX010000135         7804 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_11222_length_7804_cov_10.965802, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000135 DAOPYX010000000
VERSION     DAOPYX010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 7804)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7804)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7804
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11222_length_7804_cov_10.965802"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            6..1598
                     /locus_tag="PKN55_08035"
     CDS             6..1598
                     /locus_tag="PKN55_08035"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018150117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HNX71232.1"
                     /translation="MRIALAQFNPVVGDIAGNTSAILRLAEAARQGGAAMLLTPELAL
                     TGYPPEDLLLRDSFYAAVEKALDQLLEVDGITLVIGHPARIGAERFNAATVLRDGNRL
                     GQYHKMLLPNSEVFDECRYFTPGAAPLVVEVEGVRVGVLICEDVWHVEPAAEAAEAGA
                     QCLVALNASPFHHDKIDARQQVARYRVEETGLPLAYVNLTGGQDELVFDGASFALNRA
                     GEVAVQLPAYDDALGWVDYADGDFVAGEQAALPSRVESVYRALVVGVRDYIGKNGFPG
                     ALLGLSGGIDSALTLAVAVDALGADKVHAVMMPSPYTADISLIDSRDMVARLGAPYDE
                     LAISPMYDAFEATLAPLFAGRPVDTTEENLQSRIRGVLLMALSNKTGKLVLTTGNKSE
                     MTTGYATLYGDMAGGFAVLKDVAKTLVYELSRWRNTVSEVIPERIITRAPSAELRPDQ
                     KDQDSLPEYEVLDAIMQRYVEQNRSADEIIAEGFPEADVRKVVRLLKINEYKRRQAPV
                     GPRITQRAFGRDWRYPITNRFS"
     gene            1671..2273
                     /gene="lolA"
                     /locus_tag="PKN55_08040"
     CDS             1671..2273
                     /gene="lolA"
                     /locus_tag="PKN55_08040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018149949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HNX71233.1"
                     /translation="MTRFLLAASLSLCAALAQADGIAQLKQFVHDTRALSARFSQTVS
                     GDKPLHVSGTLELQRPGRFRWTYDTPYEQLIVGDGSRLWVYDKGLAQVTARKLDNALG
                     SSPAALLAGNNDLEKHYQLKNQGVSDGLDWLEATPKHSDGTFKSVRMGFKSGELLRMD
                     MEDNFGQHTTLTFSGLQKNPKLPASRFSFTPPKGVDVLAE"
     gene            2471..3139
                     /gene="tsaB"
                     /locus_tag="PKN55_08045"
     CDS             2471..3139
                     /gene="tsaB"
                     /locus_tag="PKN55_08045"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018746954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HNX71234.1"
                     /translation="MNTLVALDTSTEFLSLAVAVGDSVRSVHQHVGQQHAELTLPTLS
                     RLLQEQGIGLDAVDAIAFGNGPGAFTGLRIGCGLAQGLALAHEMPLIPVSTLAVLAAS
                     VSASRVLACLDARMGQVYWAAYVDGVEVCPPGLFTPEDVPLPDGEGWLLAGSGAAAHE
                     AALRVRLGAALAAVDGQASPRAEALISLARGGRWPACPPDAAELCYVRDKVALKTHER
                     LAAA"
     gene            3136..3594
                     /gene="rimI"
                     /locus_tag="PKN55_08050"
     CDS             3136..3594
                     /gene="rimI"
                     /locus_tag="PKN55_08050"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014088176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HNX71235.1"
                     /translation="MSAITVLTAEHAAELAALAAQVADYPWRAAQFVDSCAAGHQLLG
                     LRDGAGALLGFAVWSQVLDEAELLDIGVAPTARRQGLGARLLAEVIATARAAGATRLM
                     LEVRAGNQAAQALYQRAGFMVTGRRKGYYPAPDGREDACLMDKPLGDGHD"
     gene            3887..4603
                     /locus_tag="PKN55_08055"
     CDS             3887..4603
                     /locus_tag="PKN55_08055"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011137001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HNX71236.1"
                     /translation="MAPAPALTPAEPEAAGIEPASAPMAEASLFAEFADNPSPVAIPD
                     TPSSAAVALPADWDNLRAEVAACQRCRLCETRTQTVFARGNPQARWLFVGEAPGEQED
                     LQGLPFVGPAGKLLDNMLAALGLDREQDVYIANVLKCRPPRNRNPQGDEILACQPYLR
                     QQIAWQQPAVIVALGRFAAQTLLDTEASIGSLRSKVHEYAGVPLVVSYHPAYLLRTLP
                     DKAKAWQDLLFAREVMRRQG"
     gene            complement(4615..5313)
                     /gene="mtgA"
                     /locus_tag="PKN55_08060"
     CDS             complement(4615..5313)
                     /gene="mtgA"
                     /locus_tag="PKN55_08060"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014086103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HNX71237.1"
                     /translation="MTWRRLGRIALALLALLLAYQLWLLGWIVYWKWNNPAMTSFMSE
                     QQARLASQHPDDEFELKHQWVAYAAISPQLKRALVASEDAKFLQHEGFDWEGIQTAWE
                     KNLAKGRIVAGGSTISQQLAKNLFLSSQRTPWRKAEEAVITVMLEAVMDKRRIFEIYL
                     NVIEWGDGVFGAEAAARHYFRTPAARLSAAQAARLAAMVPNPRYYDTHRNDRSLARKA
                     AIIQRRMAYADVPG"
     gene            complement(5310..6131)
                     /gene="aroE"
                     /locus_tag="PKN55_08065"
     CDS             complement(5310..6131)
                     /gene="aroE"
                     /locus_tag="PKN55_08065"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011137134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HNX71238.1"
                     /translation="MTDQYAVIGNPISHSQSPLIHSEFAKQTGQNIVYDRLSAPLDGF
                     DRVASAFVGQGGKGLNITLPFKGDAFRLAHQLTSRASVCEAVNTLTFKDGVIYGDNTD
                     GVGLVRDIVENLDMPIAGKRVLLLGAGGAVRGVLLPLLEEKPASLTVANRTVEKAQAL
                     ASQFAPYGAVRAVGYPDLMGEQFDIIINGTSTSLHDELPPIPHGVFAPDALAYDMVYS
                     AGLTPFLKRAQQEKAGMLADGLGMLVEQAAESFFIWRGVKPETRRVMAILREVLN"
     gene            complement(6182..6634)
                     /locus_tag="PKN55_08070"
     CDS             complement(6182..6634)
                     /locus_tag="PKN55_08070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018746807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNX71239.1"
                     /translation="MVDVTAQLAQAAQRAQAAGLPYAGAVTPEEAHTLLNALHNAVLV
                     DVRTQAEWNFVGGVPGAVCIEWKSFPGMQPNPDFLAQLHARVPSDAHVLFLCRSGARS
                     HDAAIAATAAGYTQCYNVLEGFEGDRDAHGHRGALNGWQAAGLPWQQG"
     gene            7009..>7804
                     /locus_tag="PKN55_08075"
     CDS             7009..>7804
                     /locus_tag="PKN55_08075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase beta-grasp
                     domain-containing protein"
                     /protein_id="HNX71240.1"
                     /translation="MAVADVLKMIKDNGVKFVDLRFTDTRGKEQHVTVPAHVVDEAWF
                     ENGHAFDGSSIAGWKGIQASDMLLAPDANTANLDPFFDEPTVIMTCDVIEPSDGKGYD
                     RDPRSIAKRAEAYLKASGLGDTAYFGPEPEFFIFDSIRFGADMSGCFVEIESEEGAWS
                     SSKKYENGNTGHRPGVKGGYFPVPPVDSLHDIRAQMVLVLEELGIPVEVFHHEVATAG
                     QCEIGTKFSTLVERADWTQTLKYVVQNVAHQYGKTATFMPKPIVGDN"
BASE COUNT         1339 a         2452 c         2596 g         1417 t
ORIGIN      
        1 acaccatgcg tatcgccctt gctcaattca acccggtggt cggcgacatt gccggcaata
       61 cgtccgccat cctgcgtctg gccgaagcgg cgcggcaggg gggcgcggcg atgttgttga
      121 cgccggagct ggcgctcacc ggctatccgc cggaagacct gctgctgcgc gacagttttt
      181 atgcggcggt ggaaaaagcg ctggatcagt tgctggaggt ggacggcatc accctggtga
      241 ttggccaccc ggcacgcatc ggcgccgagc gctttaacgc cgccaccgtg ctgcgcgatg
      301 gcaaccggct ggggcagtat cacaagatgc tgttgcccaa tagcgaggtg ttcgacgagt
      361 gccgctattt cacccccggc gccgcgccgc tggtggtgga ggtggaaggc gtgcgggtgg
      421 gtgtgctgat ttgtgaggat gtctggcacg tcgagccggc ggccgaggcg gccgaggccg
      481 gcgcgcagtg cctggtggcg ctgaacgcct cgccgtttca tcacgacaag atcgacgcgc
      541 gccagcaggt ggcgcgctat cgggtggaag aaaccggcct gccgctggct tatgtgaatc
      601 tgaccggcgg ccaggacgag ctggtgttcg acggcgcgtc gtttgcgctg aaccgcgccg
      661 gcgaggtggc ggtgcagctg ccggcttacg acgacgcgct gggctgggtg gactatgccg
      721 acggtgattt tgtcgccggc gagcaggccg cgctgccgtc gcgggtggaa agcgtctacc
      781 gcgcgctggt ggtcggcgtg cgcgactata tcggcaaaaa tggctttccg ggcgcgctgc
      841 tgggcttgtc cggcggcatt gactccgcct tgacgctggc ggtggcggtg gacgcgctgg
      901 gcgcggacaa ggtgcacgcg gtgatgatgc cctcgccgta caccgccgac atcagcctga
      961 tcgacagccg cgatatggtg gcgcggctgg gcgcgccgta tgacgagctg gcgatttcgc
     1021 caatgtacga tgctttcgag gccacgctgg cgccgctgtt tgccggccgg ccggtggaca
     1081 ccaccgaaga aaacctgcaa tcgcgcattc gcggcgtgct gctgatggcg ctgtccaata
     1141 aaaccggcaa gctggtgctg accaccggca ataaatcgga gatgaccacc ggctacgcca
     1201 ccttgtacgg cgacatggcc ggcggctttg ccgtgctcaa ggacgtggcc aagacgctgg
     1261 tgtacgagct gtcgcgctgg cgcaataccg tcagcgaggt gattccggag cgcatcatca
     1321 cccgtgcgcc gtcggccgag ctgcgcccgg atcaaaaaga ccaggacagc ctgccggaat
     1381 acgaggtgct ggacgccatc atgcagcgct atgtcgagca aaaccgctcg gcggatgaga
     1441 tcattgccga aggcttcccc gaggcggatg tgcgcaaagt ggtgcgcctg ctgaaaatca
     1501 acgagtacaa acgccgccag gcgccggtgg gcccgcgcat cacccagcgc gcctttggcc
     1561 gcgactggcg ctacccgatc accaaccgtt tcagctgatg cgccgcgccg ccgcgccgaa
     1621 ctttccgggg cggcggcggc ccaatcgcca tgactttttg cggattcacc atgacccgat
     1681 ttcttctggc tgcttctttg tcgctgtgcg cggcgctggc ccaggccgac ggcatcgccc
     1741 agctcaaaca gtttgtgcac gacacccgcg cgctgtcggc gcgtttttcg caaaccgtca
     1801 gcggcgacaa gcccctgcac gtcagcggca cgctggagct gcaacgccct gggcgctttc
     1861 gctggaccta cgacacgcct tatgagcagc tgattgtcgg cgacggcagc cggctgtggg
     1921 tgtacgacaa aggcctggcg caagtcaccg cgcgcaagct ggataacgcc ctgggcagca
     1981 gcccggcggc gctgctggcc ggcaataacg acctggaaaa acactatcag ctgaaaaacc
     2041 agggcgtcag cgatggtttg gattggctgg aagccacgcc caagcacagc gacggcacgt
     2101 tcaagagcgt gcgcatgggc ttcaagtctg gcgagttgct gcgtatggac atggaagaca
     2161 actttggcca gcacaccacg ctgacgttct ctggcttgca gaaaaacccc aagctgccgg
     2221 ccagccgctt cagctttacc ccacccaagg gggtggatgt gctggcggag tgagcttgcg
     2281 atacgacgat gctggtaaaa atgccctggg tggatagccc agggcatttt ttgtggcaaa
     2341 tttgctgatt tcataaatgc cttggaataa aaaggcatgc ctgaatctgc ctgtgcggcc
     2401 ggccatgtcc ggccgcacgg ctttggtgtg gcctgctatg atgtgggcct ttgttttgac
     2461 cccgtttgcc atgaatacct tagtcgccct ggacacctcc accgaatttc tcagcctggc
     2521 cgtcgccgtg ggcgacagcg tgcgcagcgt gcatcagcat gttggtcagc agcacgccga
     2581 gctgaccttg cccaccctca gccgcttgct gcaagaacaa ggcatcggcc tggatgcggt
     2641 ggacgccatt gcctttggca atggccccgg cgctttcacc ggcctgcgca ttggctgtgg
     2701 gctggcgcag ggcttggcgc tggcgcatga aatgccgctg attccggtgt ccaccctggc
     2761 ggttctggcc gccagcgtca gcgcctcgcg cgtgctggcc tgcctggatg cgcgcatggg
     2821 ccaggtgtat tgggcggcgt atgtagacgg cgtggaggtc tgcccgccgg gcctgtttac
     2881 cccagaagat gttcccttgc cagacggcga aggctggttg ttggccggca gcggcgccgc
     2941 tgcgcacgag gcggcgctgc gcgtgcggct gggcgcggcg ctggcggcgg tggatggcca
     3001 ggcctcgccg cgcgccgagg cattgatcag cctggcgcgc ggcggccgtt ggccagcctg
     3061 cccgccagat gccgccgagc tgtgctatgt gcgcgacaaa gtggcgctga aaacccacga
     3121 gcgactggcc gcggcatgag cgccatcaca gtattgacgg cagaacacgc cgccgagctg
     3181 gcagcgctgg cggcgcaagt ggcggattac ccctggcgcg ccgcgcagtt tgtcgattca
     3241 tgcgctgccg gccatcaatt gctgggcctg cgcgatggcg cgggcgcgct gctgggcttt
     3301 gccgtgtggt cgcaggtgct ggacgaagcc gagctgctgg acatcggcgt ggccccgaca
     3361 gcgcgccgcc agggcctggg cgcgcggctg ttggccgagg tcatcgccac cgcccgcgcc
     3421 gctggcgcga cgcgcctgat gctggaggtg cgcgccggca accaggccgc gcaagccttg
     3481 tatcagcgtg ctggctttat ggtgaccggc cggcgcaaag gttattaccc ggccccggat
     3541 ggccgcgaag atgcttgtct gatggataaa cctctggggg acggccatga ctgatcgcca
     3601 cagccaaatt ttggaagcgc tgggcctggg cccacgctgg ctgccacgcc atgctttgcc
     3661 aggggctgcg ccggttgcgg cagaggaggc tgagccgccg tcgcaggcgc tggcggagtg
     3721 caccgcaccg tccgtgcctg tggcgcaagc gtcaattgag ccgctggacg agggggccgc
     3781 cctggcggca aacccaccag cagaagcgcc tgccgccccc gccgcgcacg cggaaacagg
     3841 agcgcaggca gacgcagcgc ccgcggcgct catcgacgcg cgggtattgg cgccagcgcc
     3901 cgccctgacg ccagccgagc ccgaggcggc ggggatcgaa cccgcgtccg cgccgatggc
     3961 cgaggcgtcc ttatttgccg agtttgccga taaccctagc ccggtcgcaa ttcccgacac
     4021 gcccagctca gccgctgtcg cgctgccggc cgattgggac aatttgcgcg ccgaagtcgc
     4081 cgcctgtcag cgttgccggc tgtgcgaaac ccgcacgcag acggtgtttg cccgtggcaa
     4141 cccgcaggcg cgctggctgt ttgtcggcga agcgccgggt gagcaggagg atttgcaagg
     4201 cctgccgttt gtcggcccgg ctggcaagct gctggacaat atgctggcgg cgctggggct
     4261 ggatcgtgag caggatgtgt atatcgccaa tgtgctgaag tgccggccgc cacgcaaccg
     4321 caatccgcag ggcgacgaaa tcctcgcctg tcagccgtat ttgcgtcagc aaatcgcctg
     4381 gcagcagccg gcggtgattg tggcgctggg ccgctttgcc gcgcaaaccc tgctggacac
     4441 cgaagccagc atcggcagcc tgcgcagcaa ggtgcatgaa tacgctggcg tgccgctggt
     4501 ggtcagctat cacccggctt atctgttgcg caccctgcca gacaaggcca aggcctggca
     4561 ggatttgctg tttgcccgcg aggtgatgcg ccggcagggt tgatgtgtcg agcatcagcc
     4621 cggcacatca gcatacgcca tgcgccgctg aataatcgcc gccttgcgcg ccaggctgcg
     4681 gtcgttgcgg tgggtgtcgt aatagcgcgg attgggcacc atggccgcca gccgcgcggc
     4741 ctgggcggcg gacagccgcg ccgccggggt gcggaagtaa tgccgcgcgg cggcttcggc
     4801 gccaaacacg ccgtcgcccc attcaatcac gttcaggtag atttcaaaaa tccgccgctt
     4861 gtccatcacc gcttccagca tcacggtgat cactgcttct tccgccttgc gccacggcgt
     4921 gcgctgcgac gataaaaaca ggtttttggc cagctgctgg ctgatggtcg aaccgccagc
     4981 gacgatgcgc cccttggcca ggtttttttc ccaggcggtc tggatgcctt cccagtcaaa
     5041 accttcgtgc tgcaaaaact tggcgtcttc gctggccacc agcgcgcgct tgagctgcgg
     5101 cgaaatggcc gcgtaggcca cccattggtg tttaagctcg aattcgtcgt cggggtgctg
     5161 gctggctaag cgcgcctgtt gttcgctcat gaacgaggtc atcgccgggt tgttccactt
     5221 ccagtaaaca atccagccca acagccacag ctgatacgcc agcaacagcg ccagcagggc
     5281 cagggcaatg cgccccagcc gtcgccatgt caattcagca cctcgcgcag aatcgccatc
     5341 acccggcggg tttccggctt gacgccgcgc cagataaaga aggattccgc agcttgctcg
     5401 accagcatgc ccaggccatc ggccagcatg ccggcttttt cttgctgggc gcgcttgagg
     5461 aagggggtca ggccggcgct gtacaccatg tcgtaggcca gtgcatccgg ggcgaacacg
     5521 ccgtgcggaa tcggcggcaa ttcgtcgtgc aggctggtgg aggtgccgtt gatgatgatg
     5581 tcgaactgct cgcccatcag atcggggtag cctaccgcgc gcaccgcgcc atacggggca
     5641 aactggctgg ccagcgcctg ggctttttcc accgtgcggt tggccacggt caggctggcc
     5701 ggtttttctt ccagcagcgg cagcagcacg ccgcgcacgg cgccgccggc gcccagcagc
     5761 agcacgcgct tgccggcaat cggcatgtcc aggttttcca cgatgtcgcg caccaggccg
     5821 acgccgtcgg tgttgtcgcc gtagatcacg ccatccttga aggtgagcgt gttgaccgcc
     5881 tcacacacgc tggcgcgcga ggtgagctgg tgggccaggc ggaaggcgtc gcccttgaac
     5941 ggcagggtga tgttcagccc cttgccgccc tggccgacaa aggcgctggc cacgcggtca
     6001 aagccgtcca gcggcgcgct caggcggtcg taaacaatgt tctggccggt ctgcttggcg
     6061 aattcgctgt gaatcagcgg cgactggctg tgcgagatgg ggttgccgat cacggcgtat
     6121 tggtcagtca tggtgacgtg cttcgcttca aagagagatc aagacaaacg gcatcatacc
     6181 gtcaaccctg ctgccacggc agccccgccg cctgccagcc attcagtgcg ccgcgatggc
     6241 catgggcgtc gcgatcgcct tcaaagcctt ccagcacgtt gtagcactgg gtgtagccgg
     6301 ctgccgtggc ggcgatggcg gcgtcgtgcg agcgcgcgcc cgagcggcac aaaaacagca
     6361 catgggcgtc actcggcacc cgggcgtgca actgggcaag aaaatccggg ttgggctgca
     6421 tgccgggaaa gcttttccat tcgatgcata ccgcgccggg cacgccgccg acaaagttcc
     6481 actcggcctg ggtgcgcacg tccaccagca cggcgttgtg caaggcattg agcagggtgt
     6541 gggcttcttc cggggtgact gcgccggcat agggcaggcc ggctgcctgg gcgcgctggg
     6601 cagcctgggc taattgggcg gtgacgtcca ccatgggggg ttctcctggc tggacgcgcg
     6661 ggcttgatct ggcgcgggca aaagcagcag gccttaaggc gcgcgcgcgt cgggtttgag
     6721 acaatccggt gacggaatcg agtcattatg gcaaagcctg tcgggctagg ctatgcccat
     6781 gcgccttgtc cattttggtg cgtgtgcacc agtattgtgc gatatttttt tgtatgcact
     6841 gttgcggtgc acaaagacag gcatgacggc gcgtgtggcc tatggcagaa tggccatgtc
     6901 gcttatctgt acatggcata ctttctgcta ccctcaacgg cgacctgcct ggcatggcca
     6961 ggcatggata acacagattt acgctcacac gtcttcatgg agagaattat ggcggttgct
     7021 gacgttttga aaatgatcaa ggacaacggc gtcaagttcg ttgacctgcg ttttaccgac
     7081 acccgtggca aagaacagca cgtgaccgtg ccggcccacg tggtggatga agcctggttc
     7141 gaaaacggcc acgctttcga cggctcctcc atcgccggct ggaagggcat tcaggcttcc
     7201 gacatgctgc tggctccgga cgccaacacc gccaacctcg acccgttctt cgacgagccg
     7261 accgtgatca tgacctgcga cgtgatcgaa ccgtccgacg gcaagggcta cgaccgcgac
     7321 ccgcgttcca tcgccaagcg cgctgaagcc tacctgaaag cttccggcct gggcgacacc
     7381 gcctactttg gtccggaacc ggaattcttc attttcgaca gcatccgctt tggcgccgac
     7441 atgtccggct gcttcgtgga aatcgaatcc gaagaaggcg cctggtcttc ctccaagaaa
     7501 tacgaaaacg gcaacaccgg ccaccgtccg ggcgtgaagg gcggctattt cccggtgccg
     7561 ccggttgaca gcctgcacga catccgcgcc caaatggtgc tggtgctgga agagctgggc
     7621 atcccggttg aagtgttcca ccacgaagtg gccaccgctg gccaatgtga aatcggcacc
     7681 aagttctcca ccctggtgga gcgcgccgac tggacgcaaa ccctgaagta cgtggtgcag
     7741 aacgtggccc accagtacgg caagaccgcc accttcatgc cgaagccgat cgttggcgac
     7801 aacg
//
LOCUS       DAOPYX010000136         7768 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_11293_length_7768_cov_10.504473, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000136 DAOPYX010000000
VERSION     DAOPYX010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 7768)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7768)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7768
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11293_length_7768_cov_10.504473"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(28..1116)
                     /locus_tag="PKN55_08080"
     CDS             complement(28..1116)
                     /locus_tag="PKN55_08080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX71241.1"
                     /translation="MANELEALARRHLVRLQRHTPTRQLLAMLCLAGLLILGGYFLYQ
                     SLPRQHTLRIAGSEVVSNRHYLMHILQEEVLPAGVRLHIASASDANDTMARLSRGELD
                     LALMQDADAERYPNIRHIATLPTEVLHVLARPPIKQLSELRNRQINLEPRGSEWREHA
                     VQALEFAGLHSGRDYLEANLDEEALMVLPGNVLPDAVVIASFIPSALVEFLIREHGYT
                     LLELPFPASAPHHLDWGQASQIPAFAYGAEPAMPPRNLLTVGSSLHLVGHDRIDARAT
                     RILLHTLYGPALAARLHLPFEEKHLTDSAGYPLAAGVEDFIKRNHPLISKENYDKFKS
                     LGGMLFSLASALLVALKWFRGEPVIEEE"
     gene            complement(1106..1990)
                     /locus_tag="PKN55_08085"
     CDS             complement(1106..1990)
                     /locus_tag="PKN55_08085"
                     /inference="COORDINATES: protein motif:HMM:NF024921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HNX71242.1"
                     /translation="MKKARHWLASRSGLLTLLTLAALLGMLGITWQLYTPPPPPPPLL
                     IAGGRLIQPLVAELAQRFSQQTGEEVQVEAGGSMAGMAAVKRGAIDIAMVGQPVPGLF
                     SDPQTRQHLLARNSLAFIVHHQSPVRNLSQAQIRQVLTGEVRNWKALGGPDAPIEVLT
                     WRKPALIALFVEWMVLGGQSVTHQAKQVDDASDMLASVAGNPLALGYVSLQDLPSRAA
                     VRPLSIDGAPFSRATILSGHYPYIQDMYLVGYGACKPQETAFMRFIRTPEAQAIIARH
                     NLAPVYPHATESEPHHGQ"
     gene            complement(1987..2757)
                     /locus_tag="PKN55_08090"
     CDS             complement(1987..2757)
                     /locus_tag="PKN55_08090"
                     /inference="COORDINATES: protein motif:HMM:NF024257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HNX71243.1"
                     /translation="MSIFRFCLPAALCLALGKTCAAEPDAITLHVRAAAELAPRLQAA
                     AERYMTLHRRASVVIVRGGSVFALKSLLHESADVAMVYGPPPLVMREALRERRNSLDS
                     VLIEQLPLTPVVHPGNPLREIRLDHLRAIFAGDIQDWRVLGGPAGPMTPLVETPTYGV
                     GLAWKEAMVGERGRLARSARLITAEDKAKEVARTPGAISFAAPWQLSPGLKPLRIVAD
                     TTGQARLPSAALSAWIRRDCPPAVHAFVKTLRTGQGES"
     gene            complement(2893..3924)
                     /locus_tag="PKN55_08095"
     CDS             complement(2893..3924)
                     /locus_tag="PKN55_08095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008952507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-6-deoxy-delta-3,4-glucoseen reductase"
                     /protein_id="HNX71244.1"
                     /translation="MTAQVTLQPSGHTFSVETGETVLEAATRQGVALPYSCRNGACGA
                     CKGKVTAGEVSHRDYQPHALSEAERAQGMALLCCAETQGDLTVEVREVKTSGDIQVKT
                     LPCRVESIQRVHDVAVLKLKLPVSERLQFMAGQYIDILMKDGKRRSFSLANAPHDDSA
                     LELHIRHMPGGSFSEYVFHQMKEKEIMRFQGPLGSFFLNEDSDKPILLLASGTGFAPV
                     KAIVEQALHAGVTRKMVLYWGARQAADLYMNELPQAWQAAHENFSYVPVLSEPAAEDH
                     WQGRTGLVHQAVLDDFADLSGWEVYACGAPVMVEAAHSSFIGERQLPADAFYSDAFFL
                     SKDMKPAAK"
     gene            3966..4844
                     /locus_tag="PKN55_08100"
     CDS             3966..4844
                     /locus_tag="PKN55_08100"
                     /EC_number="1.1.1.290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008952506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNX71245.1"
                     /translation="MKPSLLIVGCGDVARRAMPALSRHYRVLAVCRRAEQAAQWRALG
                     ARVVWADLDQADSLRRLAGLAQALLYTAPPPAEGQRDARARRLIAALQYHAMVPQQVV
                     YISTTGVYGDCAGAWVDETRPLNPESARAWRRVDAERAWRDYARRHGASLSILRAPGI
                     YAAERLPLERLRAGTPLALASEDGYSNHIHADDLAGLACAALRRRGGIRVYHAGDDEA
                     LATGAWFDALADHFALPRAPRLPRAQLRQQVSPALWSFLRESRRIDSQRARRELAWPL
                     RYPTVGDFLASLPAAG"
     gene            4910..5593
                     /gene="trmB"
                     /locus_tag="PKN55_08105"
     CDS             4910..5593
                     /gene="trmB"
                     /locus_tag="PKN55_08105"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011137333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine-N7-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HNX71246.1"
                     /translation="MDTPQTHRPIRSFVLRQGYMSKGQQRAYDTLMPVLGVGYQPQVA
                     DLDAVFGRAAPKVVEIGFGMGGATADIAQARPDTDYLGVEVHGPGVGSLLKLVEERQL
                     GNVRVIQHDAVEVFTHMLAPASLDGVHVFFPDPWHKKRHNKRRLIQPPFVALLASRIK
                     PGGYLHLATDWEDYAIQMLEVLSQEATLENTAEGYAPRPDYRPLTKFEQRGLRLGHGV
                     WDLVFRKRG"
     gene            5608..6105
                     /locus_tag="PKN55_08110"
     CDS             5608..6105
                     /locus_tag="PKN55_08110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008952503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1841 family protein"
                     /protein_id="HNX71247.1"
                     /translation="MFNPSRDEARRFFFDTWRKHQANDTLTDLERMTLAIILLHPEYH
                     PVLDAPARYLEREWLPEDGQTNPFLHLAMHLSIEEQLSIDQPPGIRARIDALSLQHQD
                     RHPALHDAMDGLAEMIWHAQRYQTPPDPQLYLHCLDRKLGRDPDPEVKPLPDDDGPGL
                     ILPPR"
     gene            complement(6178..6747)
                     /locus_tag="PKN55_08115"
     CDS             complement(6178..6747)
                     /locus_tag="PKN55_08115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018749543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HNX71248.1"
                     /translation="MRSSRLTSLLLAAAIAPALALPASYTPDPTHTYVYFEVGHLGYS
                     IQRGRFEQVAGKIRLDGAAQQGTLDIVVSAASVTTGFAQRDEQLRGVEFFNVARFPTV
                     TFRSTEMKFVGERPISAKGVLTLLGVSKPVELKISQFYCAPHPMSKKEMCGAEANVSI
                     KRSEYGMNRYLPGIGDEVKLAVQIEAFRD"
     gene            6947..7693
                     /locus_tag="PKN55_08120"
     CDS             6947..7693
                     /locus_tag="PKN55_08120"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014087702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNX71249.1"
                     /translation="MAITVNGVEISDAMIEYELPRHVEAANPLQHATHELVLRQLLLQ
                     EAARLGLEGQDDDERINQAIQVSIPAPQVSEADCQAWYDAHPEAFATGESVAARHILF
                     QPLDNTPLSLLRAKAEGILAEIQANPARFAELAQEHSACPSRAEGGELGMLARGQTVP
                     EFDAMIFTLEPGQIAPELVETQFGLHIIQVTERSDGQTLPFEEVRDMLGQHLMAQAHG
                     QALHQFLHVLVEQADIQGVDMTGALEPLRA"
BASE COUNT         1359 a         2546 c         2527 g         1336 t
ORIGIN      
        1 cgctttgctg agtgttatga gccgcgctta ttcttcttca atcaccggct cgccccgaaa
       61 ccatttcagc gccaccagca gcgcggaggc cagcgaaaac aacatgccgc ccagggattt
      121 gaatttgtcg tagttttctt tggaaatcag cgggtggttg cgcttgataa aatcctccac
      181 cccggccgcc agcgggtacc ccgctgagtc ggtgaggtgt ttttcttcaa acggcaggtg
      241 caggcgggcg gccagcgccg ggccgtacag ggtgtgcagc agaatgcggg tggcgcgggc
      301 gtcgatgcgg tcgtggccaa ccaggtgcag cgacgagccc acggtcagca gattgcgtgg
      361 cggcatggcg ggctcggcgc cgtaggcaaa ggcgggaatc tggctggcct gtccccagtc
      421 caggtgatgc ggggcactgg ccggaaacgg cagttccagc agggtgtaac cgtgctcgcg
      481 gatcagaaac tccaccaaag ccgacggaat gaacgacgca atcaccaccg catccggcag
      541 cacattgccg ggcagcacca tcaaggcttc ttcgtccaga ttggcttcca gatagtcgcg
      601 cccgctgtgc aggccggcaa attccagcgc ctgcaccgcg tgttcgcgcc attcgctgcc
      661 gcgcggctcc aggttgattt gccggttgcg cagctcggac aattgtttga ttggtggccg
      721 cgccagcaca tgcagcactt cggtgggcag ggtggcgata tggcggatat tgggatagcg
      781 ctcggcgtcg gcgtcctgca tcagcgccag atccagctcg ccccggctga gacgggccat
      841 ggtgtcgttg gcgtcgctgg cgctggcgat gtgcaggcgc acgccggccg gcagcacttc
      901 ttcttgcagg atgtgcatca gataatggcg gttgctgacc acctcgctgc cggcaatgcg
      961 caaggtgtgc tgacgcggca aggactgata caaaaaataa ccgcccagaa tcagcagccc
     1021 ggccaggcag agcatggcca gcagctggcg ggtgggggta tgacgttgca ggcgcaccag
     1081 atggcggcgc gccagggctt ccagttcatt ggccatggtg gggctcgctt tctgtggcgt
     1141 gggggtagac cggcgccaga ttgtgtcggg cgatgatggc ttgcgcctcg ggggtgcgaa
     1201 taaagcgcat gaaggcggtt tcttgcggtt tacaggcgcc gtagcccacc agatacatgt
     1261 cctggatata cggatagtgg ccagacaaaa tcgtggcgcg cgagaacggc gcgccgtcga
     1321 tcgacaatgg cctcaccgcg gcccgcgacg gcaaatcctg caaagacaca taccccagcg
     1381 ccagcgggtt accggcgaca ctggccagca tgtcgctggc gtcgtccacc tgcttggcct
     1441 ggtgggtgac gctttgcccg cccagcacca tccactccac aaacaaggca atcaacgccg
     1501 gtttgcgcca ggtcagcacc tcaatcggcg catccgggcc gcccagggct ttccagttgc
     1561 gcacctctcc ggtcagcacc tggcgaatct gcgcctggct gaggttgcgc acgggcgact
     1621 gatgatgaac gataaacgcc aggctattgc gcgccagcag gtgctggcgg gtttgcgggt
     1681 cagaaaacag ccccggcacc ggctggccga ccatggcgat gtcgatggca ccgcgcttga
     1741 ccgccgccat gcccgccatc gaaccgccag cctccacctg cacttcttcg ccggtttgct
     1801 ggctgaagcg ttgcgccaac tcggccacca agggctggat caagcgcccg ccggcaatca
     1861 gcaggggcgg tggcggcggg ggcggcgtgt agagctgcca agtgatgccg agcatgccca
     1921 gcagcgccgc cagggtaagc agggtgagta atccgctgcg cgaagcaagc cagtgtcgcg
     1981 cctttttcat gattcacctt gcccggtgcg cagtgttttt acaaaggcgt gcacggcggg
     2041 tgggcagtcg cgccgtatcc aggcgctgag cgccgctgat ggcagtcgcg cctgcccggt
     2101 ggtgtcggcg acaattcgca agggcttcag ccccgggctc aactgccagg gagccgcaaa
     2161 gctgatggcg ccaggcgtgc gggccacttc tttggctttg tcttccgccg tgatcagccg
     2221 cgcactgcgc gccagccggc cgcgctcgcc caccatcgct tctttccagg ccagccccac
     2281 gccataggtg ggcgtctcca ccaagggtgt cattggccca gccgggccgc ccagcacccg
     2341 ccagtcctgg atgtcgcccg caaaaatggc gcgcagatgg tccagccgaa tttcgcgcag
     2401 cggattgccc ggatgcacca ccggggtgag cggcagttgt tcaatcaaca cgctgtccag
     2461 gctgttgcgc cgctcgcgca gggcctcgcg catcaccagc ggcggcgggc catacaccat
     2521 ggccacgtcg gctgattcat gcagcaggga tttcagggca aacacgctgc cgccgcgcac
     2581 aatcaccacg ctggcgcgcc ggtgaagggt catatagcgt tcagcggcgg cttgcaggcg
     2641 cggcgccagc tcggcggcgg cgcgcacatg cagggtgatg gcgtcgggtt cagctgcgca
     2701 cgtcttgccc aaggccaggc agagggccgc aggcaggcaa aagcgaaaaa tggacataag
     2761 cgttctggcg tagaggtcag caacatcgcc gggaacgcgt tggcgtggcg ctggtgaacg
     2821 cgccaaggcc aaaacacacg gccgcacgcc gtggggcgtg cggccgtcat gggccatcag
     2881 actgtggcag gcttacttag cggccggctt catgtccttg gacaagaaaa acgcatcaga
     2941 ataaaacgca tcggccggca gctggcgctc gccgataaag ctgctgtgcg cggcctccac
     3001 catcaccggc gcgccgcagg cgtagacttc ccagccggac aaatcggcaa agtcgtccag
     3061 caccgcctgg tgcaccaggc cggtgcggcc ctgccagtgg tcttctgccg ccggctcgga
     3121 cagcaccggc acatagctga aattctcgtg cgccgcctgc caggcttgcg gcagctcatt
     3181 catatacaaa tccgccgcct ggcgcgcccc ccagtacaac accatcttac gggtgacgcc
     3241 ggcgtgcagc gcctgctcga caatggcttt taccggggca aagccggtgc cgctggccag
     3301 cagcaggatg ggcttgtccg agtcttcatt gagaaagaac gagcccagcg ggccctggaa
     3361 gcgcatgatt tccttttctt tcatctggtg aaacacatac tcggaaaaac tgccgcccgg
     3421 catgtggcgg atatgcagct ccagcgcgct gtcgtcgtgc ggggcgttgg ccagcgagaa
     3481 gctgcggcgc ttgccgtcct tcatcaggat gtcgatatat tggccagcca tgaattgcag
     3541 gcgctcggac accggcagct tgagcttgag caccgccaca tcgtgcacgc gctggatgct
     3601 ttccacccgg cacggcaggg ttttcacctg gatatcgccg ctggttttca cctcgcgcac
     3661 ttccacggtg agatcgccct gggtttcggc gcagcacagc aaggccatgc cctgggcgcg
     3721 ctcggcttcg ctcagcgcgt gcggctggta atcacggtgg ctgacctcgc cggcggtgac
     3781 tttgcccttg caggcgccac aggcgccgtt gcggcagctg tacggcagcg ccacgccctg
     3841 acgggtggcc gcttccagca cggtttcgcc ggtttcaaca gaaaaagtat gcccgctggg
     3901 ctgaagggtg acttgcgctg tcattcggtt ttgcttcctg ctggggcccg gctacaatct
     3961 ggcggatgaa gccaagctta ttgatcgtgg gatgcggcga tgtcgcccgt cgcgccatgc
     4021 cggcgctttc ccggcattac cgggtgctgg ccgtgtgccg acgcgccgaa caggcggcgc
     4081 agtggcgcgc gctgggcgcg cgcgtggtgt gggccgacct ggaccaagcc gacagcctgc
     4141 gccggctggc cgggctggcg caggcgctgt tgtacaccgc gccgccgccg gctgaaggcc
     4201 agcgcgacgc gcgcgcgcgt cggctgattg ccgcattgca atatcacgcg atggtaccac
     4261 agcaggtggt gtacatcagc accacgggcg tgtacggcga ctgtgccggc gcctgggtgg
     4321 acgaaacccg gccgctcaac cccgaatcgg cccgcgcctg gcgccgggtg gacgccgagc
     4381 gcgcctggcg cgactacgcc cgccgccacg gcgccagcct gagcatcctg cgcgcgcccg
     4441 gcatctacgc cgccgaacgc ctgccgctgg agcgcctgcg cgccggcacg ccgctggcgc
     4501 tggccagcga agacggctac agcaaccata tccacgccga cgatctggcc ggcctggcgt
     4561 gcgccgcctt gcgccgacgc ggcggcatcc gcgtgtatca cgccggcgac gatgaggcgc
     4621 tggctaccgg cgcctggttt gacgcgctgg ccgaccactt tgccctgccg cgcgcgccgc
     4681 gcctgccgcg tgcacaactg cgccagcaag tgtcgcccgc gctgtggtcg tttttgcgcg
     4741 aatcgcgccg catcgacagc cagcgcgccc gccgcgaact ggcctggccg ctgcgctacc
     4801 ccaccgtggg tgattttctg gccagcctgc cggcggccgg ctgacgcgca ctggcgcgca
     4861 cgccagccat tcggtaacat cgcgcccttt gaaatcagct ggataatcaa tggacacccc
     4921 gcaaacccat cgcccgatcc gcagctttgt gctgcgccag ggttatatgt ccaagggtca
     4981 acaacgcgct tacgacacct tgatgccggt attgggcgtg ggctatcagc cgcaggtggc
     5041 tgatctggac gccgtgtttg gccgcgccgc ccccaaggtg gtggaaatcg gctttggcat
     5101 gggcggcgcc accgccgaca tcgcccaggc ccgcccggac accgattacc tgggcgtgga
     5161 agtgcacggc cccggcgtgg gcagcctgct caagctggtg gaagagcgcc agctgggcaa
     5221 tgtgcgggtg attcagcacg acgccgtgga agtgttcacc catatgctgg ccccggccag
     5281 cctggacggc gtgcatgtgt ttttccccga cccctggcac aaaaagcgcc acaacaagcg
     5341 ccggctgatc cagccgccct ttgtcgccct gctggcctcg cgcatcaagc ccggcggcta
     5401 tctgcacctg gccaccgact gggaagacta cgccatccag atgctggaag tgctcagcca
     5461 ggaagccaca ctggaaaaca ccgccgaagg ctacgcgccg cgcccggact accgcccgct
     5521 gaccaagttc gagcagcgcg gcctgcgcct gggccatggc gtgtgggatc tggtgtttcg
     5581 caaacgcggc tgacctggag aacggcgatg ttcaacccct cgcgcgacga agcgcgccgc
     5641 tttttcttcg acacctggcg caagcaccag gccaacgaca cgctgaccga tctggagcgc
     5701 atgacgctgg ccatcatcct gctgcacccg gaatatcacc cggtgctgga tgcgccagcg
     5761 cgttatctgg agcgcgaatg gctgccggaa gacggccaga ccaatccttt tttgcacctg
     5821 gccatgcacc tgtcgattga agagcaattg tccatcgacc aaccgcccgg catccgcgcc
     5881 cgcatcgacg cgcttagcct ccagcatcaa gaccgccacc cggccttgca cgacgccatg
     5941 gacggcctgg cggaaatgat ctggcacgcc cagcgctacc aaaccccgcc cgacccgcag
     6001 ctgtatttgc actgcctgga ccgcaagctg ggccgcgacc cggaccccga ggtcaagccg
     6061 ctgccggacg acgacggccc cggtttgatc ctgccgccgc gctaagagcg gcgcttaatc
     6121 atgcaaaagc ccaccgattt gacggtgggc ttttgcatgg ccggctgacg cctggcgtca
     6181 atcgcggaag gcttcgatct gcaccgcgag cttgacttcg tcgccaatgc ccggcagata
     6241 gcggttcatg ccgtattcgc tgcgtttgat gctgacattg gcctcagcgc cgcacatttc
     6301 ttttttgctc atcgggtgcg gggcgcagta aaactggctg attttcagct cgaccggctt
     6361 gctcaccccc agcagtgtca gcacgccctt ggccgagatc ggccgttcgc caacaaattt
     6421 catttcggtg gagcgaaacg tcactgtcgg aaagcgggca acgttaaaaa actccacgcc
     6481 gcgcaattgc tcgtcacgtt gggcaaagcc ggtggtgacg gatgccgcgc tgaccacgat
     6541 gtccagcgtg ccttgctggg cggcgccatc cagccggatt ttgccggcca cctgctcaaa
     6601 acggccgcgc tggatggaat aacccagatg ccccacttca aaatacacat aggtgtgggt
     6661 cgggtcgggg gtgtagctgg cgggcagggc cagcgccggg gcgatggcgg cggccagcag
     6721 caggctggtc aggcgggacg agcgcatcgg gtctccttgt tgattcgggg cggtggggcg
     6781 gccgctggcc gcgcgcgatg ccgtaagggt agcaaaagcc gggcgcgggg gcgacagcca
     6841 gacgcgcaat ttgcgcgcgc cacagtggcc gccctccggg caagcgcgca taatcgcccg
     6901 gtcgcgccgg cgggtcggtg cgaccgacct tcatgaaagc gcgcttatgg ccattactgt
     6961 aaacggcgtg gaaatctccg acgccatgat tgaatacgaa ctgccgcgcc atgtcgaggc
     7021 agccaatccg ctgcaacacg ccacccacga actggtgctg cgtcagttgt tgttgcaaga
     7081 ggccgcacgg ctggggctgg agggtcagga tgatgacgag cgcatcaatc aggccattca
     7141 ggtgtccatt ccggccccgc aagtcagcga agccgattgc caggcctggt acgacgcgca
     7201 ccctgaggcc tttgccaccg gtgaatcggt ggcggcccgg catattttgt tccagccgct
     7261 ggataacacc ccgctgtcgc tgctgcgcgc caaggccgag ggcattctgg cagaaatcca
     7321 ggccaacccg gcgcgctttg ccgagctggc gcaagagcac tcagcctgcc cgtcgcgcgc
     7381 ggaaggcggc gagctgggca tgctggcgcg cggccagacc gtgccggaat ttgacgccat
     7441 gatctttacc ctggagccgg gccagattgc ccccgaactg gtggaaaccc agttcggcct
     7501 gcacattatt caggtgaccg agcgcagcga cggccagacg ctgccgtttg aagaagtgcg
     7561 cgacatgctg ggccagcacc tgatggcgca agcccatggc caggcgctgc atcagttttt
     7621 gcatgtgctg gttgagcagg ccgacatcca gggcgtggac atgaccggcg cgctggagcc
     7681 gctgcgcgcc tgaggtcaag agccgctcac aaaaccctcc tggcgttgtt gcacgacctt
     7741 gccgtactac ccgtactgtc tgcggtcg
//
LOCUS       DAOPYX010000137         7727 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_11379_length_7727_cov_8.714937, whole genome shotgun sequence.
ACCESSION   DAOPYX010000137 DAOPYX010000000
VERSION     DAOPYX010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 7727)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7727)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7727
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11379_length_7727_cov_8.714937"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(158..937)
                     /gene="yaaA"
                     /locus_tag="PKN55_08125"
     CDS             complement(158..937)
                     /gene="yaaA"
                     /locus_tag="PKN55_08125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004565762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HNX71250.1"
                     /translation="MLMVISPAKTLDYDTPPNTARHTLPDYLARSAELIAQLRELTPA
                     AIAQLMHISDPLAALNAGRFAEWTPAFTPAVAKQAVLAFMGDVYEGMDAQAMSEAQLD
                     WLQDHLRILSGLYGLLRPLDLMRPYRLEMGTKFANAAGKDLYAFWGDTLTEALNALLA
                     EHDSRVLVNLASDEYFKAVKPKKLAVPVVTPVFQDLKNGQYKIISFYAKRARGLMVRY
                     AALNGIDHPDGLRDFDLEDYQFVPAGSDEHTLLFRRDQAPG"
     gene            complement(989..2671)
                     /locus_tag="PKN55_08130"
     CDS             complement(989..2671)
                     /locus_tag="PKN55_08130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011136514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HNX71251.1"
                     /translation="MLKETLVAMRDLPRLREISAILIRHGLGEFAQRLKLPKAMERAT
                     EWLRVPLPDPEEFIETPVRVRRAFEELGPTFIKFGQILSTRVDVFPPDWIAEFEKLQT
                     DVPPLPLEVLQPWLFKLCQGDPHEVFAEFDYTPIGSASIAQVHRARLHDGTPVAVKVR
                     RPGIAETIEADLRILSHLAYLVELEFPDTRRYQPGEMINQFAKSLRRELDLAAEARNM
                     ERFAQHFANDPVIVVPKVYWQHTHARMNVQDFIEGIPASKLEQADAAGLDRVTLAARG
                     ADAVLKMILLDGFFHADPHPGNVFFLPENRIVFIDFGMVGRLSPARRDELVDLLAALS
                     RRNERGILEVLLEWTGSEAVDESQLHADIGEFLFQYDQVQLKDLNLSMLINDVMALMR
                     DHSIMLPSDLSMLFKALITLEGFARQINPHFQMVEHLTPFVRKIILDRYTPSTLLEKG
                     GRTVMELLGMVGGLPRDLSRLSKEIRRGKFRIDIDVKRLDHFGQQLDKSANRLTLGIV
                     TGCLIIGSSIVMTVKAGPMLFGLPLLGFLGFVVAFFNSLWLMASIWRSGKDL"
     gene            2765..3772
                     /locus_tag="PKN55_08135"
     CDS             2765..3772
                     /locus_tag="PKN55_08135"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HNX71252.1"
                     /translation="MKLTVRTLALLCALLLPHAYAAPGNDGESAGKSADAPHASAAGE
                     GAVGDMILQAVSLMGIAYRFGGNTPVNGFDCSGFIRYVFQSSGVNLPRSAAEQAQVGR
                     PVSRDELKPGDVLFFNTRGFNYSHNGLYLGNGRFIHAPRTGKNIEITSLNASYWSSRF
                     NGARRMVKGAGGVVAHEVTDDVKRAESLGRKREREETSTLADAGAAAGVAGVGAVAAA
                     AAASPRVHATLRSKDAEVKIPTLSAACKQARNAKTAKCKTEKQAIAKAEKSAKAAGKG
                     KYTLSKGESGKNIKGKKGRDKAEAKSSAKGNSKYRAAASSKDKGKGKAAAVKPTNKKK
                     R"
     gene            3769..3984
                     /locus_tag="PKN55_08140"
     CDS             3769..3984
                     /locus_tag="PKN55_08140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018152377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX71253.1"
                     /translation="MSALNFPVWCNPEGQPVACVEKIKVMHENLEELRQMAQDALEDA
                     ILMGCDEHQVRAFMVGMMQALENPYQA"
     gene            4250..5173
                     /locus_tag="PKN55_08145"
     CDS             4250..5173
                     /locus_tag="PKN55_08145"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018749170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HNX71254.1"
                     /translation="MSMADRDGFIWFDGKLVPWREANTHVLTHTLHYGMGVFEGVRAY
                     ETAQGPAIFRLQDHTDRLFRSAHILGMSLPFSKEEINAAHLDVVKANGLKSCYFRPMA
                     FYGSGKLGVAPKKDDVQVIVAAWPWGAYLGEDGLEKGIRVKTSSFTRHHVNITMCKAK
                     ANGNYMNSILANNEATADGYDEALLLDVEGYVAEGSGENIFIIRKGKLYTPDLTSALE
                     GITRDTVVTIAQEMGLQLVEKRITRDEVYSADEAFFTGTAAEITPIRELDRRQIGEGK
                     RGPITTEIQRRYFACVKGEDQNHADWLTLAV"
     gene            5235..5438
                     /locus_tag="PKN55_08150"
     CDS             5235..5438
                     /locus_tag="PKN55_08150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017508367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-finger domain-containing protein"
                     /protein_id="HNX71255.1"
                     /translation="MSELKENRARYIEVNADNLPLHCPMPDMVSWNQHPRVFLPIVQN
                     GGEALCPYCGTRYKLVGEVKGHH"
     assembly_gap    5627..5726
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            5777..6805
                     /gene="waaF"
                     /locus_tag="PKN55_08155"
     CDS             5777..6805
                     /gene="waaF"
                     /locus_tag="PKN55_08155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011135640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HNX71256.1"
                     /translation="MRNILVIAPSWVGDAVMAEPLYRRLAERWPGAAIDLYAPAWTLP
                     LAARMDCVRQGVVNPFGHGALRLAERWREGRALAAHGYDQAIVLPNSLKSALIPWFAG
                     IPQRTGFVGEFRRGVLNDARRLDKTALPTMVERFCALAEDAGAPPKRPIAHPRLNVSA
                     QAREAALQACGLSAGQPVMALCPGAEYGPAKRWPAAHFARLAQDALARGWQAWVFGSG
                     KDQAIADEIVRLAGAPTGLVNLCGRTGLDQAIDLMSLARLAVCNDSGLMHIAAALRVP
                     LVALYGSSSPGFTPPLSDQAEILSLKLDCSPCFKRECPLGHTHCLTQLTPDQVWPAVG
                     RLLPDSLP"
     gene            6802..7407
                     /locus_tag="PKN55_08160"
     CDS             6802..7407
                     /locus_tag="PKN55_08160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008953936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNX71257.1"
                     /translation="MTRKAAPPQAEDNSRRAELVRTAARLFRDQGYERTTVRDLGNAV
                     GLQSGSLFYHFRNKEEILVAVMALGITSTTEQLAAALEVAKTPYDKVSALIHVHLNSL
                     LGENQAALEVMLYEWRSVSAEQKPGLIVLRDRYEALWQSALDEAAAAGVVGQDTRLLR
                     RMVLGSLHWSVQWFRRDGGDSVDALAERMLKLVIGPRPQAD"
     gene            complement(7483..>7727)
                     /locus_tag="PKN55_08165"
     CDS             complement(7483..>7727)
                     /locus_tag="PKN55_08165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012697388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNX71258.1"
                     /translation="NAELGLFAEVRGMGLLLGAVLNDHYAGRAGEVVAKAAEAGVIVL
                     QAGPNVVRFAPSLVIGEDEVSEALGRLGSALQAFAG"
BASE COUNT         1489 a         2495 c         2354 g         1289 t
ORIGIN      
        1 cctcgacctt ctgattacaa gtcagctgct ctaccaactg agctacgccg gcgaagaggg
       61 cgaattatgc cccagacgcc gcctgagcgc aagtcgccat gctcttgtgc tgacctgcat
      121 cagcgccttc ggcgcccgcc gtcagactgc gcaatactca acccggcgcc tggtcgcggc
      181 ggaacagcag cgtgtgttca tccgagccgg ccggcacaaa ctgatagtct tccagatcaa
      241 aatcgcgcag gccgtccggg tggtcgatgc cgttcagcgc ggcgtagcgc accatcagcc
      301 cgcgtgcgcg tttggcataa aaactgatga ttttatactg gccgtttttc aggtcctgaa
      361 acaccggggt gaccaccggc acggccagtt ttttcggttt gaccgccttg aagtattcat
      421 cgctggccag gttgaccagc acgcggctgt cgtgctcggc cagcaaggcg ttcagcgctt
      481 cggtcagcgt gtcgccccag aaggcgtata aatccttgcc ggcggcgttg gcgaatttgg
      541 tgcccatttc cagccgatag gggcgcatca ggtccagcgg gcgcagcagg ccgtacaggc
      601 cggacaggat gcgcaggtgg tcttgcagcc aatccagctg cgcttcgctc atggcttgcg
      661 cgtccatgcc ctcgtacaca tcgcccataa acgccagcac cgcctgcttg gccaccgccg
      721 gggtgaaggc tggcgtccac tcggcaaagc ggccggcgtt cagcgccgcc agcgggtcgc
      781 tgatgtgcat cagctgggcg atggcggccg gggtcagttc gcgcagttgg gcaatcagct
      841 cggccgagcg cgccagatag tcgggcaggg tgtggcgcgc ggtgttgggc ggggtgtcgt
      901 aatccagggt tttggcgggg gaaatcacca tcaacatggc ttgggcctcg ggggagggat
      961 cagggatggc ggcattgtag cactgacgtc acaagtcctt gcccgaacgc cagatcgacg
     1021 ccatcagcca caggctattg aagaaggcca cgacaaagcc gagaaaaccc agcaagggca
     1081 ggccaaacag catcggcccg gccttcaccg tcatgacgat ggacgagccg ataatcaggc
     1141 agccggtgac aatgcccagg gtcagccggt tggcgctttt atccagttgc tggccaaaat
     1201 ggtccagccg cttgacgtcg atgtcgatgc gaaacttgcc gcgacggatt tccttgctca
     1261 gccgcgacaa atcgcgcggc aggccgccca ccatgcccag cagctccatc accgtgcgcc
     1321 cgcctttttc cagcagggtg gatggcgtgt agcggtcgag gatgatcttg cgcacaaacg
     1381 gcgtcaggtg ctccaccatc tgaaaatgcg ggttgatctg gcgggcaaag ccttccaggg
     1441 tgatcagcgc cttgaacagc atggacaaat ccgagggcag catgatcgag tggtcgcgca
     1501 tcagcgccat cacgtcattg atcagcatcg acagattcag gtctttcagc tgcacctgat
     1561 cgtactgaaa caaaaactcg ccaatatccg catgcagctg cgattcatcc accgcctcgc
     1621 tgccggtcca ctccagcagc acttccagaa tgccgcgctc attgcgccgc gacaaggccg
     1681 ccagcaaatc caccagctca tcgcgccggg ctggcgacaa gcggccgacc atgccaaagt
     1741 cgataaacac aatgcggttt tccggcagaa aaaacacatt gcccggatgt gggtcggcat
     1801 gaaaaaaccc gtccagcaga atcattttca gcaccgcatc ggcgccgcgc gccgccagcg
     1861 tcacccgatc cagcccggca gcgtcggcct gctccagctt gctggccgga atcccctcga
     1921 taaaatcctg cacattcatc cgcgcgtggg tgtgctgcca gtacaccttg ggcaccacaa
     1981 tcaccgggtc gttggcaaaa tgctgggcaa agcgctccat attgcgcgct tccgccgcca
     2041 gatccagctc gcggcgcagc gatttggcaa actggttgat catctcgccc ggctgatagc
     2101 ggcgggtgtc gggaaactcc agctccacca gatacgccag gtgcgagagg atgcgcaaat
     2161 ccgcctcgat ggtttcggca atgcccggcc ggcgcacctt caccgccacc ggcgtgccat
     2221 cgtgcaggcg ggcgcggtgc acctgggcaa tcgaggcgct gccgatgggc gtgtagtcga
     2281 actcggcaaa cacctcgtgc gggtcgccct ggcacagctt gaacagccag ggttgcagga
     2341 cctccagcgg cagcggcggg acgtcggttt gcagtttttc gaattcggca atccagtcgg
     2401 gcggaaacac atccacccgc gtggacagaa tctggccaaa cttgataaag gtcggcccca
     2461 gttcttcaaa ggcgcggcgc acccgcaccg gggtttcgat gaattcttcc gggtcgggca
     2521 gcggcacgcg cagccattcg gtggcgcgct ccatggcctt gggcagtttc agccgctggg
     2581 cgaattcgcc caggccgtga cgaatcagga tggcgctgat ttcgcgcagg cgcggcaagt
     2641 cgcgcatggc gaccagggtt tctttgagca tgaaaacatt cccgcccgac aggggcgatg
     2701 gcacagcagg gcaacagccc gcgcggcttt acagcgcgga catgcttcga ttagagtgca
     2761 gtctatgaaa ttgaccgtcc gcacgctagc cctgctctgc gccctgttgc tgccacacgc
     2821 ctacgcggcg ccaggcaatg acggcgaatc cgccggcaaa tccgccgatg ccccgcacgc
     2881 ctcggcggct ggcgaaggcg cggtaggcga tatgatcctg caagccgtca gcctgatggg
     2941 cattgcctac cgctttggcg gcaatacccc ggtaaacggc tttgattgca gtggctttat
     3001 ccgctatgtc ttccagtcct ccggcgtcaa cctgccgcgc tcggcggccg aacaagccca
     3061 ggtgggccgc ccggtcagcc gcgacgagct caagcccggc gacgtgctgt tcttcaacac
     3121 ccgtggcttc aattactcgc ataacgggct gtatctgggc aatggccgct ttatccacgc
     3181 cccgcgcacc ggcaagaaca tcgaaatcac cagcctgaac gccagttact ggagcagccg
     3241 cttcaatggc gcccggcgca tggtcaaggg cgcgggcggg gtggtggcgc atgaagtcac
     3301 cgacgacgtc aaacgcgccg aaagcctggg ccgcaagcgc gagcgcgaag aaaccagcac
     3361 cctggccgat gccggcgctg ccgccggcgt ggcaggcgtt ggcgccgtgg ccgccgctgc
     3421 ggcagccagc ccgcgcgtgc acgccaccct gcgcagcaaa gacgccgaag tgaaaatccc
     3481 cacgctatcg gccgcctgca agcaagcgcg caacgccaaa accgccaagt gcaaaacaga
     3541 aaaacaagcc atcgccaagg cagaaaaatc cgccaaggcc gccggcaagg gtaaatacac
     3601 cctgagcaag ggcgaatccg gcaagaacat caagggcaag aaggggcgcg acaaggccga
     3661 agccaagtcg tccgccaagg gcaacagcaa ataccgcgcc gccgccagca gcaaggacaa
     3721 aggcaaaggc aaggccgccg cggtcaaacc gaccaacaag aaaaaacgat gagcgcgttg
     3781 aatttcccgg tgtggtgcaa cccggagggc cagccagtgg cctgcgtgga aaaaatcaag
     3841 gtgatgcacg aaaacctgga agaactgcgc cagatggcgc aggacgccct ggaagacgcc
     3901 atcctgatgg gctgcgacga acaccaggtg cgcgccttca tggtgggcat gatgcaggcg
     3961 ctggaaaacc cgtatcaagc ttgaaccacg ggcctaaagc cgcctcactc ccattggcgc
     4021 ttgccgtact ccccatgctg tctgcgggca cacacccggc caggggcgct tggctgggtg
     4081 tgacgggcgg cgcctcacgt ttcccattct tgagctggcc attctgccga gccgggctga
     4141 catggcctcc tgatcccgac gttttttcac gcccccctgc gccatccgcc cggatggcgc
     4201 tataatcccc gattattttg cacacactag cgagtgaacg gagtatctca tgtcgatggc
     4261 tgaccgcgac ggtttcatct ggtttgacgg caaactcgtg ccctggcgcg aggcgaacac
     4321 ccatgtgttg acccacaccc tgcactacgg catgggcgtg ttcgagggtg tgcgcgccta
     4381 cgaaacggcg caaggcccgg cgattttccg tttgcaggat cacaccgacc gcctgttccg
     4441 ctcggcccat atcctgggca tgagcctgcc gttcagcaaa gaagaaatca acgccgccca
     4501 cctggacgtg gtgaaggcca acggcctgaa atcctgctac ttccgcccga tggcgtttta
     4561 tggctcgggc aagctgggcg tggcgccgaa aaaagacgat gtgcaagtga tcgtcgccgc
     4621 ctggccgtgg ggcgcctacc tgggcgaaga tggcctggaa aaaggcatcc gcgtgaaaac
     4681 cagctcgttc acccgccatc acgtgaacat caccatgtgc aaggccaagg ccaacggcaa
     4741 ctacatgaac tccatcctgg ccaataacga agccaccgcc gacggctacg acgaggcgct
     4801 gctgctggat gtggaaggct atgtggccga aggctcgggc gagaacatct tcatcatccg
     4861 caagggcaag ctgtacaccc cggatttgac cagcgcgctg gaaggcatca cccgcgacac
     4921 cgtggtgacc atcgcccagg aaatgggcct gcaactggtg gaaaaacgca tcacccgcga
     4981 cgaagtgtac agcgccgacg aggctttctt taccggcacc gccgctgaaa tcaccccaat
     5041 ccgcgagctg gaccgccgcc agattggcga aggcaagcgt ggcccgatca ccaccgaaat
     5101 ccagcgtcgc tactttgcct gcgtgaaggg cgaagaccag aaccacgccg actggctgac
     5161 cctggcggtt taagccgcac gccgaccggt ctgccggtcg gcgcttcccc cactccgacc
     5221 ttccgagcac gaccatgagc gaactcaaag aaaaccgcgc ccgctacatc gaagtcaacg
     5281 ccgacaacct gcccctgcac tgcccgatgc ccgatatggt gtcctggaac cagcacccgc
     5341 gcgtgttcct gcccatcgtg caaaacggcg gtgaagccct ctgtccgtac tgcggcaccc
     5401 gctacaagct ggtgggcgaa gtcaagggcc accactgatc cggtgagcca acgcgcctga
     5461 gcgccgctgg cgcgcgggca gcaaggaagg tgtggcgcag ccgcaccttt tttacttgga
     5521 ggggcgcttt tttaccgcac agcgccgatt cctcgccaag ttttatccgc agtttttatt
     5581 cgggcgtcgt ttgcgcaggc aggcgacgac aagacacacg catgcgnnnn nnnnnnnnnn
     5641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5701 nnnnnnnnnn nnnnnnnnnn nnnnnngttt ttattcgggc gtcgtttgcg caggcaggcg
     5761 acgacaagac acacgcatgc gaaacatcct cgtcatcgcc ccatcctggg tgggcgacgc
     5821 cgtgatggcc gagccgctgt accgccggct ggccgaacgc tggccagggg ccgccatcga
     5881 cctgtacgcc ccggcctgga cgctgccgct ggccgcgcgc atggactgcg tgcgccaggg
     5941 cgtggtcaat ccgtttggcc atggcgcgct gcggctggcc gagcgctggc gcgaaggccg
     6001 cgcactggcc gcccacggct atgaccaggc gattgtgctg cccaattcgc tgaagtcggc
     6061 gctgattccg tggtttgccg gcatcccgca gcgcaccggc tttgttggcg agtttcgtcg
     6121 cggcgtgctc aatgacgccc gccggctgga caaaaccgcg ctgccgacca tggtcgagcg
     6181 cttttgcgcg ctggccgaag acgctggcgc gccgcctaag cggccgattg cccatccccg
     6241 gctgaatgtg tcggcgcagg cgcgcgaggc ggctttgcag gcctgtggtt tgtccgccgg
     6301 acaaccggtg atggcgctgt gccccggcgc cgaatacggc ccggccaagc gctggccggc
     6361 ggcgcatttt gcccggctgg cgcaggatgc gctggcgcgc ggctggcagg cctgggtgtt
     6421 tggctcgggc aaagatcagg cgattgccga cgagattgtc cgcctggctg gcgcgcccac
     6481 cgggctggtg aatctgtgcg gccgcaccgg cctggatcag gcgattgatc tgatgtcgct
     6541 ggcgcggctg gcggtgtgca atgactccgg cctgatgcat attgccgccg ccttgcgcgt
     6601 gccgctggtg gcgttgtatg gttcgtccag ccccggcttt accccgccgc tgtctgacca
     6661 ggccgagatt ctcagcctga aactggattg cagcccgtgt tttaaacgcg agtgcccact
     6721 ggggcacacg cactgcctga cgcagctcac ccccgaccag gtgtggcctg ccgtcgggcg
     6781 acttcttcct gatagtttgc catgacgcga aaagccgccc cccctcaagc cgaagacaac
     6841 agccgccgcg ccgagctggt gcgcaccgct gcgcgcctgt tccgcgacca gggctacgaa
     6901 cgcaccacgg tgcgcgacct gggcaacgcc gtgggcctgc aatcaggcag cctgttctat
     6961 cactttcgca acaaggaaga aatcctggtg gcggtgatgg cgctgggcat caccagcacc
     7021 acggagcaac tggccgccgc gctggaagtg gcgaaaaccc cttacgacaa agtctcggcg
     7081 ctgattcatg tgcacctgaa ttccctgctg ggcgaaaacc aggccgcgct ggaagtcatg
     7141 ctctacgagt ggcgcagtgt gtcggccgaa caaaaacccg gcctgatcgt gctgcgcgac
     7201 cgctacgaag ccctgtggca aagcgcgctg gacgaagccg ccgccgctgg cgtggtcggc
     7261 caggacaccc ggttgctgcg ccgcatggtg ctgggcagcc tgcattggtc ggtgcagtgg
     7321 ttccgccgtg atggtggcga ctcggtggat gcgctggccg agcgcatgct gaaactggtg
     7381 attggcccgc gtccgcaggc ggactgagcg gatacaccag acaaaacggc gcggggcgct
     7441 tgggcaatca gcccagcgcc ccgcgtcgtt tatgctgtgc gatcagccgg caaacgcttg
     7501 cagcgcactg cccaggcggc ccagcgcctc gctgacttcg tcctcgccaa tcaccagcga
     7561 cggcgcaaag cgcaccacat tcgggccagc ctgcaacaca atcaccccgg cctccgcggc
     7621 cttggccacc acttcgccag cgcggccggc gtagtggtca ttcagtacgg cacccagcaa
     7681 caaccccatc ccgcgcacct cggcaaacag ccccaattca gcattca
//
LOCUS       DAOPYX010000138         7461 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_11990_length_7461_cov_8.456792, whole genome shotgun sequence.
ACCESSION   DAOPYX010000138 DAOPYX010000000
VERSION     DAOPYX010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 7461)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7461)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7461
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11990_length_7461_cov_8.456792"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..720)
                     /locus_tag="PKN55_08170"
     CDS             complement(<1..720)
                     /locus_tag="PKN55_08170"
                     /inference="COORDINATES: protein motif:HMM:TIGR01760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HNX71259.1"
                     /translation="MADQAAVLRDFLVRLGFKIDTASQSRFTAGVAAATKAVARLGAA
                     AIAAAVTVAAATAKMTGALDDLYYASRRTGASANNIKAFGYAVSQMGGDADSAAASLE
                     AFARNLRSSPGYFGMLEKLGVQAKDAGGNLRDTVDVLGDVGKALSAMPYYQANAYAQA
                     LGIDERTLMAMREGVGEFSDEYRRILAATGTDLDKSAKNANDAMRAWRTFSAVSGLVL
                     SRLGKSILDALQPAMRWFSANA"
     gene            complement(713..838)
                     /locus_tag="PKN55_08175"
     CDS             complement(713..838)
                     /locus_tag="PKN55_08175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX71260.1"
                     /translation="MCRYESLLNGALGIADIALMNDALDVRADNDYLMRESMKDG"
     gene            complement(910..1371)
                     /locus_tag="PKN55_08180"
     CDS             complement(910..1371)
                     /locus_tag="PKN55_08180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX71261.1"
                     /translation="MTELEIGGNTYRINSMPAMTQFHVMRKLGPVLPGIVPVLAQAAA
                     ALPQADGQEDGGAMTTVDGVAAIAMAARPLLDGLAAMADADAEYVINHCLSAVMRRDP
                     GGKSWNAVSRDGVTMFDDIDLMTSMQIVWAVLRENYTGFFRAASTALAPAQ"
     gene            complement(1374..1823)
                     /locus_tag="PKN55_08185"
     CDS             complement(1374..1823)
                     /locus_tag="PKN55_08185"
                     /inference="COORDINATES: protein motif:HMM:NF023110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3277 family protein"
                     /protein_id="HNX71262.1"
                     /translation="MATYSFMDVAATIVGPGGIVDLGYGAATAEEGISIAYTEDKNLM
                     TVGADGKVMHTLRCGNSGTVTVSLLKTSPTAKKLQAMFNIQKLSSSVWGVNTITIQHT
                     VAKDKIVCLFCAFVKVPDTAYAKDGDVLQWAFHAGEIAVDRGTYPGN"
     gene            complement(1833..3413)
                     /locus_tag="PKN55_08190"
     CDS             complement(1833..3413)
                     /locus_tag="PKN55_08190"
                     /inference="COORDINATES: protein motif:HMM:NF023291.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3383 domain-containing protein"
                     /protein_id="HNX71263.1"
                     /translation="MAIPTLGLPVSKLVRVDVFMSPQAAATRSFGSLLICGDSDVIDV
                     TERIRQYDTVESVGLDFGADSPEYKAAVLYYSQRPRPADLYIGRWATEPTQARLNGAL
                     LDEYQTDIAIWQAISNGGFDIAIDGTNRTITGLDFSGVSNLNAVAGIITDALAGKAVC
                     VWNATLRRFEITANSTGSTSVLGYATKPSYAVGLATEAGQQINTEAGAHIIVTSGSGA
                     TDISGMLGLADGMAYPPVDGIDSETPEECAMRMVDLSADWYGLMFAARKPITTKQHVD
                     VAALIEASEISHIYGITTDNKAVLDASITTDIASRLRSLSYQRSFVDFSTTARYTAAA
                     IFGRAFSVNFRASNSTITLMFKEQVGVPSEKLSAQQAATLKKKNCNVFVLYNNGKAIL
                     QHGTMAGGWFFDERHGLDWLQNLMQTEVFNLLYTSNTKIPQTEQGMGYIETTLAKCCG
                     QALTNGLIAPGIWNSDPIGDELDRGAALSTGFYIYHQPIADQFQSDREQRKAPVFQVA
                     LKLAGAVHSVDVLVAVNR"
     gene            complement(3417..3959)
                     /locus_tag="PKN55_08195"
     CDS             complement(3417..3959)
                     /locus_tag="PKN55_08195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX71264.1"
                     /translation="MANTSATGGYLRPELPWLAGDDLDDVFQRLTVGITGLPGSMVRP
                     RWQADPPKTPEHDQDWVALGVTVETPDAGPSIQHRPAGNGHDVETRHFGIEVQFSCYG
                     PHAAALAQAIRSGLSIPQNIEQIRPIGFVSTGQAVRVPELVNQRHYNRVDFTAFFRLA
                     RQQRYAVLNVLEVPLQLNKD"
     gene            complement(3959..4333)
                     /locus_tag="PKN55_08200"
     CDS             complement(3959..4333)
                     /locus_tag="PKN55_08200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX71265.1"
                     /translation="MTIDVTELLTDPDFVMHDLVAISRAQSVNDAGRTENTEVRTTFS
                     GVVFPGTGNMMLRTPDGERVTGDISIVTRHYLTDGIGQGRTADVVEYAGGRYIVKNTF
                     DYSNWGRGFVMAVCDMLPVGGV"
     gene            complement(4339..4809)
                     /locus_tag="PKN55_08205"
     CDS             complement(4339..4809)
                     /locus_tag="PKN55_08205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX71266.1"
                     /translation="MQSGMQMITDALPDLLRRLAAVAQRDVLVGVPAGEARDDGPTNA
                     EIGYQNEFGSPANNIPARPHLLPGVAAVQDKVVAKLTRAADAAATGRPSDAERHLHAA
                     GLLAQNSVRRTLTTTAYRPLSERTLAERRARGRTGTKPLIDTGQYRRSITYVVR"
     gene            complement(4819..5199)
                     /locus_tag="PKN55_08210"
     CDS             complement(4819..5199)
                     /locus_tag="PKN55_08210"
                     /inference="COORDINATES: protein motif:HMM:NF024659.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4054 domain-containing protein"
                     /protein_id="HNX71267.1"
                     /translation="MVDIATFRAVFPELGDSAKYPDAQVQYYLDFAAQSLRPEAWRNL
                     LERGTGLFVAHYLAMSAISRAGTVPGRGQLGIVASKSVGPASISYDNSAISAQVDAGH
                     WALTSYGLMYWELMRMVGTGGWQL"
     gene            complement(5199..5402)
                     /locus_tag="PKN55_08215"
     CDS             complement(5199..5402)
                     /locus_tag="PKN55_08215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX71268.1"
                     /translation="MQAKAKISFHVEHPDGSGTVHFVRGQVYDIPDDSELLKHWYVKA
                     CTERPPQPKSVSVTPPATGKPKG"
     gene            complement(5412..6500)
                     /locus_tag="PKN55_08220"
     CDS             complement(5412..6500)
                     /locus_tag="PKN55_08220"
                     /inference="COORDINATES: protein motif:HMM:NF021467.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2184 domain-containing protein"
                     /protein_id="HNX71269.1"
                     /translation="MAHRVYLGDQGGRSYTLPDKCLPANFNPATAYQSAFARRFIGDQ
                     TSNVIRQMLGDAGAYFIGQLEALDQRMAMPITAVTYSRDIERRPDIGPYNELASFTRL
                     KFAAAQSDRDDIDFDSDVTTEYQQVNVEQEKITTPIFAKTMKTRWTLAEIEKAALAGM
                     PLETQTFEALNTDNQLKIDRLHYMGSQKYGFGGLLNHGSVLNVSPVANGAGGLPNFES
                     KTPEEILADFNEILRSVWVATGLAIMPNRILVSQRIMSALSIPIAPLSGVSILKYLQD
                     NAICKIIDGKDLEIVPVKWASGAGAGGTDRMTAYVPGLDNLASIFSPMQQYGAMEVRD
                     VAFHQHYRFAFGGLQLRYPEKMAYRDGL"
     gene            complement(6512..7030)
                     /locus_tag="PKN55_08225"
     CDS             complement(6512..7030)
                     /locus_tag="PKN55_08225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX71270.1"
                     /translation="MDPIVQSNVYPRSLPSGIPGRISRGNPTIESGDPDESKPFAAYG
                     LPVKKVAGRVVPIEAGDAADVVFGFLVESFPTSDTRLVTRGVLSGSVPVAYNPHAVLR
                     RGYMHVLANGGAAYETADGGQVYVRIANPMPGLPIGGIEALNSPDVVAIPRAFFKGRR
                     DQAKNVEIEYNI"
     gene            complement(7030..>7461)
                     /locus_tag="PKN55_08230"
     CDS             complement(7030..>7461)
                     /locus_tag="PKN55_08230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX71271.1"
                     /translation="EDQAPDPLPEAMPKIVTGDSAADVLAKCKVLFPAGRYQTGDSDS
                     VKTQNMRQALAGVQFTPAGLAAKSAMLGDARPEELKGDAMAMAFNGLYAVIAAKADAE
                     KEAAVRHVMGDRLAPTEQKETPEQRHDRLHAEFWAKQKGGK"
BASE COUNT         1280 a         2303 c         2365 g         1513 t
ORIGIN      
        1 ggcattggcg ctgaaccagc gcatggctgg ctgcagggcg tccagaatgc tcttgccgag
       61 ccgcgacaat accagtccgg acacggcgct gaacgtccgc catgcgcgca tggcgtcatt
      121 ggcgtttttt gccgacttgt ccaggtctgt accggtggcc gccaggatgc gccggtactc
      181 gtccgagaac tcgccaacgc cttcgcgcat cgccattagc gtgcgctcgt caatcccaag
      241 cgcctgggcg tatgcgtttg cctggtagta cggcatggcg gaaagcgcct tgccgacatc
      301 gcccagcaca tctaccgtgt cgcgcaaatt gccgcccgcg tccttggctt ggacgcccag
      361 cttttccagc ataccgaaat agccgggcga gctgcgcaag ttgcgtgcga atgcctccag
      421 agacgcggca gcgctatccg catcaccgcc catctggctc acggcatagc caaacgcctt
      481 gatgttgttg gcgctggccc cggtgcggcg actggcataa tacagatcgt ccagcgcgcc
      541 ggtcattttt gctgtggcag cggcaacagt taccgctgcg gcgatagcgg ctgcaccaag
      601 ccgggccacc gccttggtgg cagcggccac gccagcagta aaccggctct ggcttgccgt
      661 gtcgattttg aagcccagcc ggacaaggaa atcccgcagg acagcagcct ggtcagccat
      721 ctttcatgct ctcccgcatc agataatcgt tgtctgcgcg cacgtccagc gcgtcattca
      781 tcagcgcgat gtcggcaatg cccagcgcgc cgttcagcag tgattcgtat cggcacatgc
      841 cggccagcac cgggcgcaat agccagtcct cgccatccgg caggctgcgc cactcaaccc
      901 gtccgctgct tactgagcgg gcgcaagggc ggtactggct gcgcgaaaaa aacccgtgta
      961 gttctcgcgc agcacggccc agacgatttg catgctggtc atcaggtcga tgtcgtcaaa
     1021 catggtcacg ccgtcgcggc tgacagcatt ccaggatttg ccgccagggt cgcggcgcat
     1081 gacggctgac aggcaatggt tgatgacata ctcagcgtcg gcatcagcca tggccgccag
     1141 gccatcaagc agcgggcggg ccgccatggc gatggcggca acgccatcca cggtggtcat
     1201 ggcgccgccg tcttcctgac cgtcggcctg gggtagtgcg gcagcggcct gggccagcac
     1261 cgggacgatg cccggcagca ccgggcccag cttgcgcatg acatggaact gtgtcattgc
     1321 cggcatgctg ttgatgcggt aggtgttacc gccgatttcg agttcagtca ttgtcagtta
     1381 cccggatatg tgccgcgatc aacggcgatt tcaccagcat gaaacgccca ctggagcacg
     1441 tcgccgtctt tggcgtacgc ggtgtctggg accttcacga atgcgcagaa caggcagacg
     1501 attttgtctt ttgccaccgt atgctgaatg gtgatggtgt tcacgcccca tacgctggag
     1561 ctcagcttct ggatgttgaa catcgcctgg agctttttgg ccgtcggact ggttttcagc
     1621 aggctgacgg taacggtgcc gctgttgccg cagcgcagcg tgtgcattac cttgccgtcc
     1681 gccccgacgg tcatcaggtt tttgtcttcg gtgtaggcaa tcgagatgcc ttcttctgcc
     1741 gttgccgcgc catagccaag gtcaacgatg ccgcccgggc caacaatggt ggcggccacg
     1801 tccatgaacg aatatgtagc catgatttgc ctttatcggt tgacggccac cagcacatcc
     1861 acggaatgga ctgcgccggc caatttgagt gctacttgaa aaaccggagc cttgcgctgc
     1921 tcgcggtcgg actggaactg gtcggcaatg ggctggtggt agatgtaaaa cccggtggac
     1981 agcgccgcgc cgcgatccag ttcatcgcca atcggatcgg aattccagat gcccggcgca
     2041 atgagcccgt tggtcagcgc ctggccgcag cacttggcca gcgtggtttc gatgtatccc
     2101 atgccctgct cggtctgcgg gattttggtg ttgctggtgt acagcaaatt aaacacctcg
     2161 gtttgcatca ggttctggag ccagtccagg ccgtgacgct catcgaagaa ccagccgccg
     2221 gccatggtgc cgtgctgcaa aatcgccttg ccattgttgt acagcacaaa cacgttgcag
     2281 tttttcttct tgagcgtagc ggcctgctgg gcgctcagct tctcgctggg cacgcccacc
     2341 tgttccttga acatcagcgt gatggtggaa ttgctggcac ggaagttgac gctgaacgcc
     2401 cggccgaaaa tcgcggcggc ggtgtatcgg gccgtggtgc tgaaatcaac gaacgaacgc
     2461 tggtagctca gcgaccgtag ccggctggcg atgtcggtgg tgatcgacgc atccagcacg
     2521 gccttattgt cggtggtgat cccgtaaata tggctgattt cgctggcctc gatcagcgcg
     2581 gccacgtcga cgtgctgctt ggtggtgatc ggcttacgcg ccgcaaacat caggccgtac
     2641 cagtcagccg acaggtccac catgcgcatg gcgcactctt ctggcgtttc gctgtcgatg
     2701 ccatcgaccg gcggatacgc catgccgtcg gccaggccca gcatcccgga gatgtcggtg
     2761 gcgccgctgc cgctggtcac aatgatatgg gcgccggcct cggtgttgat ctgctggccg
     2821 gcctcggtgg ccaggccaac agcataggat ggcttggtag catagcccag caccgacgtg
     2881 gagccggtgc tgttggcggt gatctcgaag cggcgcagcg tcgcattcca gacgcatacg
     2941 gccttgccgg ccagcgcgtc ggtgataatg ccggccacgg cgttgaggtt gctcacgccg
     3001 ctgaaatcca gcccggtgat ggtgcggttg gtgccgtcaa tggcgatgtc aaacccgcca
     3061 ttgctgatag cctgccagat ggcgatgtcg gtctggtatt catccagcag cgcgccgttc
     3121 aggcgtgcct gcgtcggctc ggtggcccag cggccgatgt acaaatcggc cgggcgcggg
     3181 cgctgactgt agtacagcac ggcggccttg tattcgggcg agtcagcgcc aaaatcgagg
     3241 cccaccgatt caacggtgtc atattggcga atgcgctcgg tcacgtcgat gacgtcgcta
     3301 tcgccgcaga tcagcagcga cccgaacgag cgggtggcgg ccgcctgagg cgacatgaac
     3361 acatcgacgc ggaccagctt ggaaaccggc aggcccaggg tagggatggc catgatttag
     3421 tccttgttga gttgcagggg cacttccagc acgttgagca ctgcatagcg ctgctggcgc
     3481 gccaggcgga aaaacgcagt gaaatccacc cggttgtagt gccgctggtt gaccagctcg
     3541 ggcacgcgca cggcctggcc ggttgagaca aacccgattg gtcggatttg ctcgatgttt
     3601 tgcgggatgc tcaggccgct gcggattgcc tgggccagcg cggcggcgtg cggtccgtag
     3661 cacgaaaact gcacctctat gccgaagtgc cgcgtttcga cgtcgtgacc atttccggct
     3721 gggcggtgct ggatagacgg cccggcatcc ggcgtttcca ccgttacgcc cagcgccacc
     3781 cagtcctgat cgtgctcggg ggtctttggc gggtctgcct gccagcgcgg gcgcaccatg
     3841 ctgcctggca gcccggtaat gccgacggtc aggcgctgga aaacatcgtc caggtcatcg
     3901 ccggcaagcc acggcaattc tggccgcagg tagccgccag ttgctgatgt gttggccatc
     3961 aaacaccccc gactggcagc atgtcgcaaa cggccatcac aaagccgcgc ccccagttgc
     4021 tgtaatcaaa ggtgtttttg acgatgtacc gcccgccggc atactccacc acatccgccg
     4081 tccgcccctg gccaatgccg tcggtcaggt aatgccgggt cacaatgctg atatcgccag
     4141 ttacccgctc gccatccggc gtgcgcagca tcatgttgcc ggttcccgga aatacaaccc
     4201 cggaaaaggt ggtgcgtacc tcggtgttct cggtgcgccc ggcgtcgttg acggactgcg
     4261 cccggctgat tgccaccagg tcatgcatca caaaatccgg gtccgtcaac agctcggtta
     4321 cgtcaatggt catgcggatt acctgacaac gtaggtgatt gaccggcggt attgcccggt
     4381 gtcaatcagt ggttttgttc cagtgcgtcc gcgcgcccgt cgctcggcaa gtgtgcgctc
     4441 gctgagcggg cggtaggccg tcgtggtgag cgtgcggcgc acgctgtttt gcgcgagcaa
     4501 tccagcggcg tgcaagtggc gctcggcatc ggacgggcgg cctgttgcgg cagcgtcggc
     4561 ggcccgggtc agtttggcga ccaccttgtc ttgcacggca gccaccccgg gcagcagatg
     4621 gggccgcgca gggatgttgt ttgccgggct gccaaactca ttctggtagc cgatttccgc
     4681 attggtcggg ccgtcgtcgc gcgcctcccc ggcgggcacg cccaccagaa catcacgctg
     4741 agccacggcg gccagccggc gcagcagatc gggcagcgca tcagtgatca tttgcatgcc
     4801 tgattgcatg tctgcctcct aaagctgcca tccgcccgtg cccaccatcc gcatcagctc
     4861 ccagtacatc aagccatagc tggtcagtgc ccagtgccca gcatctacct gggcgctgat
     4921 tgcgctgttg tcatagctga tgctggccgg gccgactgac tttgaggcga caatgcctag
     4981 ctgcccccgt ccggggactg ttccggcccg gctgattgcc gacattgcca gatagtgcgc
     5041 gacaaacagc ccggtgccgc gctccagcag gttgcgccat gcctctggcc gcagtgattg
     5101 cgcggcaaag tccaggtaat actgcacctg ggcatccggg tattttgccg aatccccgag
     5161 ctcggggaat acggcgcgga aggtggcgat gtccaccatc agcccttggg cttgccggtg
     5221 gccggcggcg tgacgctaac cgacttgggc tgaggcgggc gctcggtgca ggccttgacg
     5281 taccagtgct tcagcagctc gctgtcgtcc gggatgtcat agacctggcc ccgcacaaaa
     5341 tgcaccgtgc cgctgccgtc gggatgttcc acgtggaacg aaatttttgc tttggcttgc
     5401 atgccgtctc cttacaggcc gtcgcggtag gccatttttt ccgggtagcg gagttgcagc
     5461 ccgccgaagg caaaacggta gtgctggtgg aacgcgacgt cgcgcacctc catggcgcca
     5521 tactgctgca tcggcgagaa aatcgaggcc aggttatcga ggccaggaac gtaggccgtc
     5581 atacggtcgg tgccacccgc gccagcgccg gatgcccatt tcaccgggac gatttccagg
     5641 tccttgccgt cgatgatttt gcagatggcg ttgtcttgca ggtacttcag aatcgacacc
     5701 ccggacagcg gcgcaatcgg gatggacagc gcgctcatga tgcgctgaga caccagaatc
     5761 cggttgggca tgatggcaag gccggtggcc acccagacgc tacgcaaaat ctcgttgaag
     5821 tccgccaaaa tctcttccgg cgttttgctt tcgaaattcg gcaagccgcc ggcgccgttt
     5881 gccaccgggc tgacgttcag gacgctgccg tgattcagca ggccgccgaa accgtatttc
     5941 tggctgccca tgtagtgcag ccggtcgatt ttcagctggt tatcggtgtt gagcgcttcg
     6001 aacgtctggg tctccagcgg catgccggcc agcgcggcct tttcgatctc ggccagggtc
     6061 caccgggttt tcatcgtttt ggcgaaaatc ggggtggtga ttttctcctg ctcgacgttg
     6121 acctgttggt actcggtcgt cacgtcggag tcgaagtcga tatcgtcccg gtcggactgg
     6181 gcggcggcga acttgaggcg ggtgaagctg gcgagctcgt tgtacggccc gatgtccggg
     6241 cggcgctcga tgtcgcggct gtaagtcact gccgtgatcg gcattgccat gcgctggtcc
     6301 agcgcctcca gctgcccaat gaaataagcg ccggcatcgc ccagcatctg gcggatgacg
     6361 ttgctggttt ggtcgccgat gaaccggcgg gcaaacgcag actggtaggc cgtcgctggg
     6421 ttgaaattcg ccggcaggca tttgtcaggc agcgtgtagg agcggccacc ctggtcgcca
     6481 agataaactc gatgtgccat gtgtcaggcc tttagatgtt gtattcgatt tcgacgtttt
     6541 tcgcctggtc acggcggccc ttgaaaaagg cgcgcgggat ggcgacaacg tctgggctgt
     6601 tcagtgcctc gatgccgcca atcggcaggc cgggcatcgg gttggcgatg cgcacataga
     6661 cctggccgcc atcggcggtt tcgtaggctg caccgccgtt ggccagcaca tgcatgtatc
     6721 cgcgccgcag cacggcatgc gggttgtacg ccaccggcac gctgccggac aacacgccgc
     6781 gcgtgacaag ccgcgtgtcg ctggtcggga acgactcgac cagaaaacca aacaccacat
     6841 ccgcagcatc gccggcttcg atcggcacga cgcgcccggc cacctttttt accggcagcc
     6901 cgtaggccgc aaacggcttg ctttcgtcgg ggtcgccaga ttcgatggtt ggattgccgc
     6961 gcgagatgcg gccggggatg ccggacggca gcgagcgcgg atatacgttc gattggacaa
     7021 tcgggtccat tatttgccac ctttctgctt ggcccaaaac tcagcgtgca gccggtcgtg
     7081 gcgctgctcg ggggtttctt tttgctcggt cggggccaga cggtcgccca tcacatggcg
     7141 cacggctgcc tctttttccg cgtccgcctt ggcggcaatc acggcgtaaa ggccgttgaa
     7201 ggccatcgcc atggcgtcgc ctttcagttc ttcgggccgg gcatcgccca gcatggcgga
     7261 tttcgctgcc aagccggcgg gcgtgaactg caccccagcc agcgcctggc gcatgttctg
     7321 agtttttacg gaatcgctgt cgccggtctg gtagcggcca gccgggaaca gcaccttgca
     7381 tttggccagc acatcggcgg cgctatcgcc ggtcacgatt ttgggcatgg cctccggcag
     7441 cgggtcgggc gcctgatctt c
//
LOCUS       DAOPYX010000139         7452 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_11999_length_7452_cov_10.877923, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000139 DAOPYX010000000
VERSION     DAOPYX010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 7452)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7452)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7452
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11999_length_7452_cov_10.877923"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..447)
                     /locus_tag="PKN55_08235"
     CDS             complement(<1..447)
                     /locus_tag="PKN55_08235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011135506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HNX71272.1"
                     /translation="MSFSPELLLPAGTLDKMRAAYDFGADAVYAGQPRYSLRARNNEF
                     KLEQLASGIAEAHARGKKFYVASNLLPHNAKVKTYLADMAPVIAMQPDALIMADPGLI
                     MMVRETWPDMPVHLSVQANTVNYMGVKFWQKLGVSRIILSRELSLDE"
     gene            complement(581..1213)
                     /locus_tag="PKN55_08240"
     CDS             complement(581..1213)
                     /locus_tag="PKN55_08240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018749021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HNX71273.1"
                     /translation="MHTSTLLLYLIAVSAVMITPGPSMLLALNNGACYGMRVALWGMA
                     GAVLSDLLLIAAVGCGLGALLQTSEQLFSVVKWLGAAYLLYLAWGMWRAPAQPLRAEA
                     LAGGGQGGAAAFWRAVLVGLSNPKGVLFFSAFLPQFIRPDAPMAEQYLVLALISAVVD
                     CLMMSLYAFGGRHAMRTFSARVMRGVNRGCAGLLAAMAVGLTLYRRSDAV"
     gene            complement(1309..2160)
                     /gene="umuD"
                     /locus_tag="PKN55_08245"
     CDS             complement(1309..2160)
                     /gene="umuD"
                     /locus_tag="PKN55_08245"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF012920.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HNX71274.1"
                     /translation="MAGRKRKTPKWVDGKPSFDDMPVHYQVEQALLAERLLAGLEEGR
                     LTLNEPRQHAAARTALRALLAAEHAEHERPVAEWVDAQLNAASRTKLLGALRQERFRA
                     SRGIRQVALKPSEFQQLQILRERAEQRRRAPLSAPDRAMLHELGAILPPHLTEVVSVP
                     LYSHTVPAGQPAPADDDIEDLIDLNDYLMPRRDASFLVRVKGESMVDAGIRDGDLLVV
                     DRSRPPVDGKIVVAALDGELTVKRLSTRHGRVQLLPANPAFAPIDIDEGCELMIWGVV
                     RNVIHSL"
     gene            2281..2622
                     /locus_tag="PKN55_08250"
     CDS             2281..2622
                     /locus_tag="PKN55_08250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012697533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HNX71275.1"
                     /translation="MASCSPQSICMQCGACCAAFRVTFHVSELDSEGGRVPAGLADAE
                     TGTLYRLRGTDYARPRCLALVGKIGEAVHCGIYHERPSPCREFGARAEIGVFEDACHR
                     ARALHGLPALE"
     gene            complement(2729..3238)
                     /locus_tag="PKN55_08255"
     CDS             complement(2729..3238)
                     /locus_tag="PKN55_08255"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNX71276.1"
                     /translation="MLSSRSALLAVQPMQASDLPAILAIQRLCYPAHLIEPAAALQSR
                     RQRAPGHCWVARRGGQVLGYLFSHPWQRTSLPKLGQDLPALPPAPDCLFLHDLAVHPD
                     GRGQKVAEQLLHASYRPARAAGLRHGRLVAVEGADAFWARHGYRRQSEAASGLSGYGE
                     QAVLMDCLL"
     gene            complement(3373..3939)
                     /locus_tag="PKN55_08260"
     CDS             complement(3373..3939)
                     /locus_tag="PKN55_08260"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HNX71277.1"
                     /translation="MPLNLETLRLVAQLHHAYQNRQRDPLTQAFARSSGEEVLALQFE
                     VAERSNTPLTVAVLDIDRLSEVNERHSHPHGDQLLADLPKHIAKTQRQGDMLVRWSGK
                     RFLLMLPNTSLEHARIALERFRKHGFGLRPDGQPVTLSVGVAERQADALASWTALVEK
                     AEGRALRVKQEGRDGVRGDDEVQHAHAA"
     gene            complement(4267..5565)
                     /gene="aceA"
                     /locus_tag="PKN55_08265"
     CDS             complement(4267..5565)
                     /gene="aceA"
                     /locus_tag="PKN55_08265"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004451 - isocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HNX71278.1"
                     /translation="MTTREQRIAAIEKDWAENPRWAGIKRGYTAADVERLRGSFPVEY
                     TLARRGAEKLWNLVNNEPYINCLGALTGGQAMQQVKAGIKAIYLSGWQVAADNNEYSA
                     MYPDQSLYPVDSVPKVVERINNAFTRADEIQHSKGIEAGDKGYIDYHAPIVADAEAGF
                     GGVLNAFELMKSMIRAGAAGVHWEDQLASVKKCGHMGGKVLVPTQEAVQKLIAARMAA
                     DVYGVPTLVIARTDAEAADLLTSDYDENDKPFLTGERTAEGFYKTKKGLDQAISRAIA
                     YADYADLVWCETGTPDLEFARKFAEAVHAKHPGKMLAYNCSPSFNWKKNLDDATIAKF
                     QRELGAMGYKYQFITLAGIHSMWYNMFDLAQDYVARGMSAYVEKVQEPEFAARERGYT
                     FVSHQQEVGTGYFDDVTTVIQGGKSSVTALTGSTEEEQFH"
     gene            complement(5573..5896)
                     /locus_tag="PKN55_08270"
     CDS             complement(5573..5896)
                     /locus_tag="PKN55_08270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX71279.1"
                     /translation="MTVFLLSCNAKFSAAEHRFSFTKMRHFFIAAAHTARFFYGLIPY
                     TKLYESLQNRGENTRFGWYPVVAIHHNRAQADTAALAIFALLQGKTGITGMSPVLGQF
                     EYILG"
     gene            6055..6579
                     /gene="grpE"
                     /locus_tag="PKN55_08275"
     CDS             6055..6579
                     /gene="grpE"
                     /locus_tag="PKN55_08275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008953241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HNX71280.1"
                     /translation="MQSQDNPQQPAADELNAQQAQAPADASARVAELEAQVAELKDQY
                     LRTRAEQENQRRRAAEDLQAAHKFAVQKFAAELLPVKDSLELALADQSGQFDALKNGV
                     DLTLKQLASAFEKFQLLEINPQGEKLDPHKHQAISMVDAAGAEANTVVHVMQKGYQIA
                     ERVLRPAMVVVAKG"
     gene            6767..>7452
                     /locus_tag="PKN55_08280"
     CDS             6767..>7452
                     /locus_tag="PKN55_08280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008953240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HNX71281.1"
                     /translation="MGKIIGIDLGTTNSCVAVLENNQPKVIENAEGARTTPSIIAYQE
                     DGEILVGAPAKRQAVTNPKNTLYAVKRLIGRRFEDKEVQKDIDLMPFSIVKADNGDAW
                     VGVRDKKLAPPQISAEVLRKMKKTAEDYLGEEVTEAVITVPAYFNDSQRQATKDAGRI
                     AGLEVKRIINEPTAAALAFGMDKMEKGDRKIAVYDLGGGTFDISIIEIADVDGDKQFE
                     VLATNGDTFLG"
BASE COUNT         1509 a         2341 c         2269 g         1333 t
ORIGIN      
        1 ctcgtccagg ctcaactcgc gcgacaggat aatgcgcgac acgcccagct tctgccagaa
       61 cttcaccccc atatagttga cggtgttggc ctgcacgctc aggtgcaccg gcatgtcggg
      121 ccaggtttcc cgcaccatca tgatcaggcc ggggtcggcc atgatcagcg cgtccggctg
      181 catggcgatc accggcgcca tatcggccag ataggttttc accttggcgt tgtgcggcag
      241 cagattggag gccacgtaaa atttcttgcc gcgcgcatgc gcctcggcaa tgccgctggc
      301 cagttgctcc agcttgaact cattattgcg cgcgcgcagg ctgtagcgcg gctggccggc
      361 gtacaccgca tcggcgccaa aatcgtaggc ggcgcgcatc ttgtccagcg tgccggcggg
      421 caacagtaac tcgggggaaa aagacatggt cgggctgcgt cgggcggtgg gagcgcccag
      481 ggaaataaac ccgcgattat ccgcagccct ctggccggcg tcaagccggc cgggcttgat
      541 gggtggaatt taaccctatg aattacatgg ttttatcggc tcaaacggca tcgctgcgcc
      601 gatacagcgt cagccccacc gccatggcgg ccagcaggcc ggcgcagcca cggttcaccc
      661 cgcgcatcac cctggccgaa aacgtgcgca tggcgtggcg gccgccaaag gcgtacaagc
      721 tcatcatcag gcaatccacc accgcgctga tcagcgccag caccaggtat tgctcggcca
      781 tcggcgcgtc cgggcggatg aattgcggca aaaaagccga gaaaaacagc acacccttgg
      841 ggttggacaa accaaccaac accgcccgcc agaacgccgc cgcgccgccc tgtccgccgc
      901 cggccagcgc ctcggcgcgc aagggctgcg ccggcgcgcg ccacatgccc caggccagat
      961 acagcaaata cgccgcgccc agccatttca ccacgctgaa cagctgctcg gaggtttgca
     1021 acagcgcgcc caggccgcag cccaccgcag caatcagcag caaatccgac agcaccgcgc
     1081 cggccatgcc ccacagcgcc acgcgcatgc cgtagcaagc gccattgttc agcgccaaaa
     1141 gcatggaagg gccaggcgtg atcatcaccg cgctgactgc gatcaaatag agcaataaag
     1201 tggaagtgtg catgacaaaa ccggcaattt cagcaaagtg ccgattgagc gccaaagcac
     1261 gcgcatcgtc aagtcatcct ggcgacagat tcagacacat ctggggcatt acagcgagtg
     1321 aatcacattg cggaccacgc cccagatcat caactcgcag ccttcgtcga tgtcgatcgg
     1381 ggcaaatgcc gggttggctg gcaacagctg cacgcggcca tggcgggtgg acaggcgctt
     1441 gacggtcagt tcgccatcca gcgccgccac cacgatcttg ccatctaccg gcgggcgtga
     1501 acgatccacc accagcaaat caccatcacg gatgccggca tccaccatcg attcgccctt
     1561 cacccgcacc aaaaaactgg cgtcacggcg cggcatcaga taatcgttga ggtcgatcag
     1621 gtcttcgatg tcatcgtctg ctggcgcggg ttgcccggca ggtacggtgt ggctgtatag
     1681 cggcacgctg accacctcgg tcaggtgtgg cggcaggatg gcgcccagct cgtgcagcat
     1741 ggcgcggtct ggtgccgaca agggcgcgcg gcggcgctgc tcggcgcgct cgcgcagaat
     1801 ttgcagctgc tgaaactcgc tgggcttgag cgccacctgg cggatgcccc gactggcgcg
     1861 aaaacgctct tgccgcaagg cgcccagtaa tttggtgcgg ctggcggcat tcaactgcgc
     1921 atccacccac tcggctaccg gccgctcgtg ttcggcatgc tcagcggcca gcagcgcgcg
     1981 caaggcagta cgcgcggcgg catgctggcg cggctcgttc agcgtcagcc ggccttcttc
     2041 cagtccggcc agcagccgct cggccagcag tgcctgctcc acctgataat gcacaggcat
     2101 gtcgtcgaaa ctgggtttgc catctaccca tttcggggtt ttgcgttttc tgcctgccat
     2161 gatcggcctc tgattgcgtg actgtgacga attatatgcg taattacacg gtaattaaac
     2221 ccataccggc aaatcgcgct aagcttgcgg gttttctggc tgcacacaca ggatttcgcc
     2281 atggcttctt gctctcccca atcgatctgc atgcaatgcg gcgcgtgttg cgcggcattc
     2341 cgggtgacgt ttcatgtctc ggagctggat agcgaagggg ggcgggtgcc ggctgggctg
     2401 gccgacgccg aaaccggcac cttgtatcgc ctgcgcggca cggattatgc gcggccacgg
     2461 tgtttggcct tggtgggcaa gattggcgag gcggtgcact gcgggattta tcacgagcgg
     2521 ccgtcgccct gtcgcgagtt tggcgcgcgt gccgagatcg gcgtgtttga agacgcctgt
     2581 caccgggcgc gggcactgca tggtttgccg gcgctggagt gattccctgc cgtcaaccgc
     2641 aaaagccagc ctgacggatc ttatcctgcc cacacgcgcg gctcgtgagc ccccacccgc
     2701 gcttgcctgg tgagggctca cggttggcct acagcaggca atccatcaac accgcctgct
     2761 cgccatagcc gctgagcccg ctggcggcct cgctttgccg gcgatagcca tggcgggccc
     2821 aaaaggcgtc agcgccttcc accgccacca gccggccatg acgcagcccg gcggcgcgcg
     2881 ccggccggta gctggcgtgc aacagctgct cggccacttt ctggccgcgc ccatccgggt
     2941 gcaccgccag atcgtgcaaa aacaggcaat ccggcgcagg cggcagggca ggcaggtctt
     3001 gccccagctt gggcaggctg gtgcgttgcc aagggtgaga aaacagatag cccagcacct
     3061 ggccgccccg gcgcgccacc caacaatggc caggcgcgcg ttgccggcgc gattgcagcg
     3121 cggcggcggg ttcgatcaga tgtgcgggat aacacagacg ctgaatggcc agaatggccg
     3181 gcagatcgct ggcttgcatg ggctgcacgg ccagcagggc agaacgggac gacaacacgt
     3241 gaacaacatc aaaataccgc gctcgggcgg cgggctgcgc agtctagcag atttgccctg
     3301 agccggcgaa cgcagcgccg gccggagggc cccccccatc cggccggcgt tttgttaacc
     3361 gcgcctggtc aatcaggcag catgagcgtg ttgcacttcg tcatccccgc gcacaccgtc
     3421 gcggccttcc tgtttgacgc gcagggcgcg cccttcggct ttttccacca gcgccgtcca
     3481 gctggccagc gcatccgcct gccgctcggc cacgccgacg ctcaaggtca ccggctggcc
     3541 atccgggcgc aggccaaagc catgtttgcg aaaacgctcc agcgcaatcc gggcgtgttc
     3601 cagactggtg ttgggcagca tcagcaaaaa gcgcttgccg ctccagcgca ccagcatatc
     3661 gccctggcgc tgggttttgg cgatgtgctt gggcaaatcc gccagcaatt ggtcgccatg
     3721 cgggtggctg tggcgttcgt tgacttcgct cagccggtcg atgtccagca ccgccacggt
     3781 cagcggcgtg ttgctgcgct cggccacttc aaattgcagc gccagcactt cttcgccgct
     3841 gctgcgggca aacgcctggg tgagcgggtc gcgttggcgg ttttggtacg catggtgcag
     3901 ttgggcaacc agcctgaggg tttccagatt gagcggcatg atgcgtcctc cttgtcttgg
     3961 gctgctgtcc tgtgatgctg gcggcgcccc gtatttatga agcccatcga catgccgatg
     4021 ttcgataggc gacgttttat ctccttggtt tatttatagc acggggattc cgtcacccac
     4081 agcgggccag acaaaaaatg gcgacaacat gacggaaact tgatctgggc caaaagtgaa
     4141 cgcccatgaa aaaaccccac cagggtgagc tgatggggcc agagggtcga tgccaggcgc
     4201 agaacgcctg gagtgatgcg aaatcgtaga cccaggccgc cgggctggcc ggcggcgagg
     4261 taaatcttag tggaactgtt cttcttcggt ggagccggtc agggcggtca ccgaggactt
     4321 gccgccttgg atcacggtgg tcacatcgtc gaagtagcca gtgcccactt cctgctggtg
     4381 cgacacgaag gtgtagccac gttcgcgagc ggcgaattcc ggctcttgca ccttctcgac
     4441 gtaggccgac atgccgcggg cgacgtagtc ttgggccagg tcgaacatgt tgtaccacat
     4501 ggagtggatg ccagccaggg tgatgaactg gtacttgtag cccatggcgc ccagttcgcg
     4561 ctggaacttg gcaatggtgg catcgtccag gttcttcttc cagttgaagg acggcgagca
     4621 gttgtaggcc agcatcttgc ccgggtgctt ggcatgcacg gcttcggcga acttgcgggc
     4681 aaattccaga tccggcgtgc cggtttcgca ccacaccagg tcggcgtagt cggcgtaggc
     4741 gatggcgcgg gagatggcct ggtccagacc cttcttggtc ttgtagaagc cttcggcggt
     4801 gcgctcgccg gtcaggaacg gcttgtcgtt ttcgtcgtag tcggaagtca gcaggtcagc
     4861 ggcttcggca tcggtacggg cgatcaccag ggtcggcacg ccgtacacgt cggcagccat
     4921 acgggcggcg atcagctttt gcacggcttc ttgagtcggc accagcactt tgccgcccat
     4981 gtggccacac ttcttcacgg aagccagctg atcttcccag tgcacgccag cggcgccagc
     5041 gcggatcatg gacttcatca gttcgaaggc gttcagcacg ccgccgaaac cggcttcagc
     5101 atcggccacg atcggagcgt ggtagtcgat gtagcccttg tcgccggctt cgatgccctt
     5161 ggagtgctgg atttcgtcag cgcgggtgaa ggcgttgttg atgcgctcaa ccaccttggg
     5221 cacggagtcc accgggtaca gggattggtc cgggtacatg gcggagtatt cgttgttgtc
     5281 agctgcaacc tgccagccag acaggtagat ggccttgatg ccggccttga cctgttgcat
     5341 ggcttggccg ccggtcaggg cgcccaggca gttgatgtac ggttcgttgt tgaccagatt
     5401 ccacagcttc tcggcgccac ggcgggccag ggtgtactcg accgggaaag agccacgcag
     5461 gcgctccacg tcagcggcgg tgtagccgcg cttgatgcca gcccagcgcg gattttcagc
     5521 ccagtctttt tcgatggcag cgatgcgttg ttcgcgagtc gtcatgtttc tgttacccca
     5581 gaatgtattc aaattggcct aaaacgggcg acatgcccgt gattcctgtt ttgccctgca
     5641 acaaggcaaa aatggcgagc gcggcggtgt cggcttgcgc acggttgtgg tggatggcca
     5701 caaccggata ccagccaaac cgggtgtttt cacctcggtt ttgcaggctt tcgtacaact
     5761 ttgtgtaggg aattaaacca taaaaaaacc gcgctgtgtg cgccgcagca ataaaaaaat
     5821 gtcgcatttt cgtaaatgaa aaccggtgtt cggcagccga aaattttgca ttgcaagata
     5881 gtaaaaacac ggtcatttac ctgtcacaag cgaacacgct cacccgcggc acgccttgct
     5941 ccatcagcgt ttgcctgtcg tcaggcccag agaaaaccac catcgcgcca cgacaggggg
     6001 ttgaaactcg ccataatgcc cctacttctc gcagcattat tcacggaggt cgccatgcaa
     6061 tcccaagaca accctcaaca acccgccgcc gacgaactga acgcacagca ggcgcaagcc
     6121 ccggctgacg ctagcgcgcg cgtggccgag ctggaagccc aggttgccga actcaaggat
     6181 caatacctgc gcacccgcgc cgagcaagaa aaccagcgcc gccgcgccgc cgaagacctg
     6241 caagccgcgc ataaatttgc cgtgcaaaag ttcgccgccg aactcttgcc ggtgaaagac
     6301 agcctggagc tggcgctggc cgaccagagc ggccagtttg acgcgctgaa aaacggcgtg
     6361 gatctcaccc tgaagcaact ggcctcggcg tttgaaaaat tccagttgct ggaaatcaac
     6421 ccgcaaggcg aaaagctcga tccgcacaaa catcaggcca tcagcatggt ggacgccgcc
     6481 ggcgccgaag ccaacaccgt ggtgcatgtg atgcaaaagg gctatcagat tgccgagcgc
     6541 gtgctgcgcc cggccatggt ggtggtggcc aagggctgac cccgccgcgc gcgaccggcc
     6601 aactcaagaa aagttcagca aaaccacttg aaaagccggc aagcgcactt atctggcaaa
     6661 acaagacacc caacggatga catggttgcc aagcgcagct aagcgtttga atccataccc
     6721 ggcgccgtca tcccatcagt aaaagatacg cgaaaggaat tgaatcatgg gcaagatcat
     6781 tggcatcgac ctgggcacca ccaacagctg tgtggccgtg ctggaaaaca accagccgaa
     6841 agtgattgaa aacgccgaag gcgcacgcac caccccgtcc atcatcgcct atcaagaaga
     6901 cggcgaaatc ctggtgggcg cgccggccaa gcgccaggcc gtcaccaacc cgaaaaacac
     6961 cctgtacgcc gtcaagcgcc tgatcggccg ccgcttcgaa gacaaagaag tgcaaaaaga
     7021 catcgacctg atgccgttct ccatcgtcaa ggctgacaat ggcgacgcct gggtgggcgt
     7081 gcgcgacaaa aaactggccc cgccgcaaat cagcgccgaa gtgctgcgca aaatgaaaaa
     7141 aaccgccgaa gactacctcg gcgaagaagt caccgaagcc gtgatcaccg tgccggctta
     7201 cttcaacgac agccagcgcc aggccaccaa ggacgccggc cgcatcgccg gcctggaagt
     7261 caagcgcatc atcaacgagc cgaccgccgc cgcgctggcc ttcggcatgg acaagatgga
     7321 aaaaggcgac cgcaaaatcg ccgtgtatga cctgggcggc ggcacctttg acatctccat
     7381 catcgaaatc gctgatgtcg atggcgacaa gcaatttgaa gtgctggcca ccaacggcga
     7441 caccttcctg gg
//
LOCUS       DAOPYX010000140         7404 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_12115_length_7404_cov_7.016057, whole genome shotgun sequence.
ACCESSION   DAOPYX010000140 DAOPYX010000000
VERSION     DAOPYX010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 7404)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7404)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7404
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12115_length_7404_cov_7.016057"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(13..825)
                     /locus_tag="PKN55_08285"
     CDS             complement(13..825)
                     /locus_tag="PKN55_08285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011136032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HNX71282.1"
                     /translation="MTDACLSRDLADGIATLTLNRPDKSNAFDQDMWRALREALQWAD
                     DTPEVRVVLLTGAGKNFCAGIDLAMLLNIQQAIHQECEGRKREALRRLILQLQDDISS
                     LERCRKPVIAAIHGACIGGGIDLVTAADIRFASADAYFAVREIDMGMTADVGTLQRLP
                     RIVGEGVARELCLTGRRIDAAEAAQLKLVNQVFDSQDALLAHARQVAASIAGKSPLAV
                     RGTKQVLNYSRDHSVADSLEYVANWNAAMLLSNDIQQAVMASMSKRTAQFAD"
     gene            complement(854..1453)
                     /locus_tag="PKN55_08290"
     CDS             complement(854..1453)
                     /locus_tag="PKN55_08290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014087326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNX71283.1"
                     /translation="MTASALPQPDDDRQDTKKRILDMAEDLLLTRGFNAFSYQHISSE
                     LGMKNAAIHYHYPKKNDLGVAVIQRYRRRFQRFVDGQAGQSARAQLEAYFQLSTHYYQ
                     HNQQICPSGVLSAEFHVLPSDMQAEALAFVAEMRDWATRILQHGLASGEFHFAGDARA
                     MAAVMFAALQGGLQLARLDAAILDDLKRQLCALLGLPPC"
     gene            complement(1568..1951)
                     /locus_tag="PKN55_08295"
     CDS             complement(1568..1951)
                     /locus_tag="PKN55_08295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019921299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1987 domain-containing protein"
                     /protein_id="HNX71284.1"
                     /translation="MENLYIPATASEPEVDFRFDTHSLTLKGESFPENAAAFYVPVIA
                     AVESYLAGRSDTTIQVNVTLAYFNSSSTKMLFSLFEILNNAVLSGNVVVLNWFYDEDD
                     DTILEFGEEIADDFAALEFRPQALV"
     gene            complement(1975..2559)
                     /locus_tag="PKN55_08300"
     CDS             complement(1975..2559)
                     /locus_tag="PKN55_08300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017406784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SiaB family protein kinase"
                     /protein_id="HNX71285.1"
                     /translation="MTEHTLRVNDMLQSEINSFRELERKCNVIFYYSGLFSQNIVAAM
                     AEALKQRLAHSGNKAATQRKVFSTFIEMAQNVIHYSADTFSDVDQQDDELRHGAVWIG
                     ESEGKFFIVCGNPVAASRAPRMRDRLNALQSMSQEEIKAAYKTQLRSENEAGSKGAGL
                     GFLTVARDASEPIEFHFDEQPDGKTAMFYLKAKF"
     gene            complement(2609..3892)
                     /locus_tag="PKN55_08305"
     CDS             complement(2609..3892)
                     /locus_tag="PKN55_08305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018152722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNX71286.1"
                     /translation="MPNTAVPSRLTATRRSASQVRPGINSRSLAGHLAQNCPVASPED
                     TNEQVLHLFSEHQHVGALPVVENGRPMGIINRNQFMSQLAKPFHRELYARKSCIAFMN
                     HSPLVVEHDVSLQDLSFMAVANGEKALADGFIITRDQQYIGIGAGVDLVKAIADLQAE
                     KNRLVMESINYASVIQKSFLRSSREDMAAALNDYFMHWEPRDVVGGDYYYFVKHDDGF
                     FCAVIDCTGHGVPGAFMTLIIASALNQVLKETDLHNPAAALAEVNRTVKQSLGQFDGG
                     RQGMDDGGEMHSDDGMDTAFCWVDHRSRTLTFAGAKTPLFILPPGAEDLDVIKGDKKG
                     VGYIDTPMDYAWENQTLSLKPGSRVYITTDGIIDQIGGPKQIAFGKKRLKKAIMECAS
                     KSMEQQKAHIMDTFYKYQGEHLRRDDVSIFGFRLD"
     gene            4284..4655
                     /locus_tag="PKN55_08310"
     CDS             4284..4655
                     /locus_tag="PKN55_08310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX71287.1"
                     /translation="MIPTPCWTLRLTVLCSLIILSGCRPENLHLAGDWQCGDFSLALA
                     RDGSYLLRPQQAGEPDEVRGVYRVDQEQDTLRIRYDSQHSSQQRYVYVASQYPTEGFY
                     LNTRPDLHGEPKLCLRRGGWA"
     gene            complement(4768..5757)
                     /locus_tag="PKN55_08315"
     CDS             complement(4768..5757)
                     /locus_tag="PKN55_08315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018151749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX71288.1"
                     /translation="MRISLLLASLLAATLAQAAPPVFSFGAWGDMPYAKNQDATQTEA
                     VIRSLNQSDIRFSLYDGDIKDGSSSCDDATFTRAAAMFNRLKKPLIYAVGDNEWTDCH
                     RLNNGGYDPLERLAQLRQVLANQPDSFGQTRLALTRQGPLGGKFAENVRFSYGGVVFI
                     GLNVPGSNNNLVLSDKECTHKSARTLAQCQAGNAEYAERDAANIDWLRSGFAQAGQQH
                     AAGVVVFFQADPGFDLPETEDVDESTLPQYKGYRALMQALTGLTEQYAGQVLLVHGDT
                     HFFKVDKPLYSPTRVLPNLTRLETFGSPVNHWVRVTVDTRRPEVFTLTPVMVR"
     gene            6055..7362
                     /locus_tag="PKN55_08320"
     CDS             6055..7362
                     /locus_tag="PKN55_08320"
                     /inference="COORDINATES: protein motif:HMM:NF014045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HNX71289.1"
                     /translation="MPKKTALFVDFDNVFLCLKAIDPSLADCFAKRPAEWLKQLESTD
                     RRFLIRRCYMNPSSFEEYRSYFIYSAFDVIDCPPITKQGKTLADMHMTIDIVDRLMHP
                     THFEEFMLFSADSDFTPVLQKLREYDRQTCVVLAGMTSGAYRASADEILQLAELLEKM
                     QGYAGNQAYMQNEQLQQYTPTPRTEYPIRPVRPASLPAPVAPEWLSEEKNQAVIQVLI
                     EALQNASHAIPSAKVIEILRENFGSEANNWFGFGRPASFFTAVNIYEHGVIFSQAAPG
                     YLYLADKHTPPRELATLGEWLRDPGSDPVLIQLANRICALTETPPLSPQEYRVCFQHI
                     AELLQHQGSGLNEMSKNLRDHAKDQGIALSRQDATGMLFLLERNGVNLQYAGKQADEL
                     AAIYADAVARRCATSQWQLSHDELLLLRDWLGAPAESGQPLPA"
BASE COUNT         1502 a         2140 c         2173 g         1589 t
ORIGIN      
        1 cttgatggtt gctcagtcgg caaactgcgc ggtgcgcttg ctcatgctgg ccatcaccgc
       61 ctgctgaata tcgttggaca acagcatggc ggcgttccag ttggccacgt attccaggct
      121 gtcggccacg ctgtggtcgc ggctgtaatt cagcacctgt tttgtgccgc gcaccgccag
      181 cggcgatttg ccggcgatgc tggccgccac ctggcgggcg tgggccagca gcgcgtcctg
      241 gctgtcgaac acctgattga ccaatttgag ctgggccgct tcggcggcgt cgatgcgtcg
      301 gccggtcagg cacagctcgc gcgcgacgcc ttcgccgaca atgcgcggca gccgttgcag
      361 cgtgcccacg tcggcagtca tacccatgtc gatttcacgc acggcaaagt aagcgtcggc
      421 gctggcaaag cggatgtcgg cagcggtgac cagatcaatc cccccgccga tgcaagcgcc
      481 gtggatggcg gcgatcaccg gcttgcggca gcgctccagg ctggagatat cgtcttgcag
      541 ttgcaggatc agccggcgca gcgcctcgcg cttgcggccc tcgcattctt ggtggatggc
      601 ttgctggatg ttcagcagca tggccagatc gatgccggcg caaaaatttt tgcccgcgcc
      661 ggtcagcagc accacgcgca cctcgggggt gtcgtcggcc cattgcagcg cctcgcgcag
      721 cgcgcgccac atgtcctggt caaaggcgtt ggatttgtcc gggcggttca gcgtcagggt
      781 ggcgatgccg tcggcaaggt cgcggctcag gcaggcgtcg gtcatgggtg ggcgttcctg
      841 tggtcaaggt gaatcagcaa ggcggcagac ccagcagcgc gcacagctgg cgcttcaggt
      901 cgtccaggat ggcggcgtcc agccgggcca gttgcaggcc gccttgcagg gcggcaaaca
      961 tcaccgccgc catggcgcgg gcgtcgccgg caaagtgaaa ctccccgctg gccaggccgt
     1021 gttgcagaat gcgggtggcc caatcgcgca tttcggcgac aaaggccagg gcttcggcct
     1081 gcatgtctga cggcagcaca tgaaactcgg cgctgagcac gccgctgggg cagatttgct
     1141 ggttgtgctg gtagtaatgg gtggacagct gaaaatacgc ttccagctgg gcgcgggccg
     1201 attggccggc ctggccatcg acaaagcgct gaaaacgccg gcgatagcgc tggattaccg
     1261 ccacgcccag gtcgtttttt ttcgggtagt ggtagtgaat ggcggcgttc ttcatgccca
     1321 gctcgctgga aatatgctgg tagctgaagg cgttgaagcc acgggtgagc agcagatcct
     1381 cggccatgtc gagaatgcgc tttttggtgt cttgccggtc gtcgtccggc tggggcagtg
     1441 cggaagcggt catgccgcga atttacttat cgataggtaa gttgtcaaac ccatcgctta
     1501 tgccatgtcc ggcttcctcc tgcatccatg cagcctcacg cccggctgca tgggtgttgc
     1561 atgggtttta caccagcgcc tgcggacgga attccagggc agcaaagtcg tcggcgattt
     1621 cttcaccaaa ctccaggatg gtgtcgtcgt cttcgtcgta aaaccagttg agcaccacca
     1681 cattgccgga cagtacggca ttattgagaa tttcaaacag tgaaaacagc atcttggtgc
     1741 tggagctgtt gaagtaggcc agggtgacat tcacctggat ggtggtgtcg ctgcggccag
     1801 ccaggtagct ttccacggcg gcaatcaccg gcacataaaa agcggcggca ttttccggaa
     1861 aggattcacc cttcagcgtc agggagtggg tgtcaaagcg aaagtccact tccggctcgc
     1921 tggcggtggc ggggatatac aggttttcca tgattgggct cttggaggcg tgaatcagaa
     1981 tttggctttc aggtagaaca tcgccgtctt gccgtcgggc tgttcgtcaa aatggaactc
     2041 aatcggctcg gaggcatcgc gggccacggt cagaaagccc aggccagcgc ccttgctgcc
     2101 ggcttcgttc tcgctgcgca gctgggtttt gtaggccgcc ttgatttctt cctgcgacat
     2161 gctttgcagg gcattcagcc ggtcgcgcat gcgcggggcc cggctggccg ccaccggatt
     2221 gccgcagacg atgaagaact tgccttcact ctcgccaatc cacaccgcgc catggcgcag
     2281 ctcgtcgtcc tgctggtcaa catcactgaa ggtgtcggca gaataatgaa tcacgttctg
     2341 cgccatctcg ataaaggtgg aaaacacctt gcgctgggtg gcagccttgt tgccggagtg
     2401 cgccagccgt tgtttcagcg cttcggccat ggcagcgacg atgttctggg aaaacaggcc
     2461 agagtaataa aagatcacat tgcactttcg ctccagttcg cgaaagctgt tgatctcact
     2521 ttgcaacatg tcattgactc gcagggtatg ctcggtcatc tcagtgtgcc tttgtgatat
     2581 tacgattcag tgaacccagc atccgggttt aatccagacg gaaaccaaaa atactgacgt
     2641 catcacggcg cagatgctcg ccctggtatt tgtaaaaggt gtccatgatg tgggcttttt
     2701 gctgctccat cgatttgctg gcgcattcca tgatggcttt tttcagccgc ttcttgccaa
     2761 aggcaatctg cttggggccg ccaatctgat cgatgatgcc atcggtggtg atgtacaccc
     2821 ggctaccagg cttcaggctg agggtctggt tttcccaggc gtaatccatg ggggtgtcaa
     2881 tatagcccac gcctttttta tcacccttga tcacatccag gtcttccgcc cccggcggca
     2941 ggatgaacag cggcgttttg gcgcccgcaa aggtcagcgt gcggctacgg tgatccaccc
     3001 agcagaatgc cgtatccatg ccgtcgtccg agtgcatttc gccgccgtcg tccatgccct
     3061 ggcggccacc atcaaactgc cccagcgact gcttgaccgt gcggttgacc tcggccagtg
     3121 cggcagccgg gttgtgcagg tcggtttctt tcagcacctg attcagcgcc gaggcgatga
     3181 tcagcgtcat gaaggcgccg ggcacgccgt ggccggtgca gtcgatcacc gcgcagaaaa
     3241 acccgtcgtc gtgtttgaca aaatagtagt aatcgccgcc caccacatcg cgtggctccc
     3301 agtgcatgaa atagtcattc agcgcggcgg ccatgtcctc gcggctgctg cgcaaaaacg
     3361 atttctggat cacgctggcg tagttgatgg attccatcac caggcggttt ttttctgctt
     3421 gcagatcggc tatcgccttg accagatcca ccccggcgcc aatgccgatg tattgctggt
     3481 cacgggtgat gataaaacca tcagccaggg ctttttcgcc gttggccacc gccatgaaac
     3541 tcagatcctg caagctgaca tcatgctcga ccaccagcgg tgagtggttc atgaaggcaa
     3601 tgcagctctt gcgggcgtac agctcgcggt gaaacggctt ggccagttgg ctcatgaact
     3661 ggttgcggtt gatgatgccc atcggccggc cgttttccac caccggcagc gcaccaacgt
     3721 gctgatgttc ggagaacaag tgcagcacct gttcattggt gtcctcaggc gaggccaccg
     3781 ggcagttttg cgccagatgg ccagccagtg aacgactgtt gatgccagga cgaacctggc
     3841 tggcagagcg gcgggtggca gtaagacggg aaggaacagc ggtattcggc atggcggcag
     3901 ctccatatca agccgcgcgt cgagggcgcc tggtgggcgc cggccggcgg cgcggatacg
     3961 ggataaggtg gggaagacac ggccatgcta cgaactggat gtgacgaaaa tatgttgcag
     4021 gtgcaaaaaa tccgcctggg cgccatcgtg ccagacatgc caggcaagaa aatatgcaaa
     4081 aagcgacaac aatgtgttaa aagaatggca ggaactgtta ttttgttacg taaaactacg
     4141 tagttatcta cactgtcaaa tagtacaatg ttgctcacaa aactcagcac agcaatccgg
     4201 gccaggcgta cagccagcag gccgtgcaac aacagcaggc gggttttgtc agcagttcca
     4261 atacgtgccg agccctgtct gtcatgattc ccactccttg ctggaccctc cgccttaccg
     4321 tcttgtgcag cctgatcatc ctgagcggct gccgccccga aaacctgcat ctggctggag
     4381 actggcagtg tggcgatttc agcctggccc tggcacgcga tggcagctat ctgttgcgcc
     4441 cgcagcaggc tggcgagccg gatgaggtac gcggcgtata tcgggtagat caggaacaag
     4501 acaccttgcg catccgctac gacagccagc acagcagtca gcaacgctac gtctatgtgg
     4561 ccagtcagta cccgacagaa gggttttatc tgaacacccg tcccgacctg cacggcgaac
     4621 ccaaattatg cctgcgtcgg ggcggctggg cataaagccc agcgcccgct gttgacggcc
     4681 atgcaggaat gcgacatttc tgtcgccatc cgaccgctcg tgccggaact ttattgcctt
     4741 tgcctatgcc catcaccgac aatcacgtca gcgcaccatc actggcgtga gggtgaacac
     4801 ttctggccgg cgtgtgtcca ccgtcacccg tacccagtgg ttgaccgggc tgccaaacgt
     4861 ttccagccgg gtcagattgg gcagcacccg ggtcgggctg tacagcggct tgtccacctt
     4921 gaagaaatgg gtgtcaccgt gcactagcaa tacctggcca gcgtactgct cggtcagccc
     4981 ggtcagtgcc tgcatcaggg cacgatagcc cttgtactgc ggcagggtgc tttcatccac
     5041 gtcttcggtt tctggcaggt caaaaccggg gtcggcctga aaaaacacca ccacaccggc
     5101 cgcatgctgc tggccggcct gggcaaagcc gctgcgcagc caatcgatat tggcagcgtc
     5161 ccgctcggca tactcggcat tgcccgcctg gcactgcgcc agagtgcgtg cgctcttgtg
     5221 cgtgcactct ttgtcgctca gcaccagatt gttgttgctg cccggcacat tcaggccgat
     5281 gaacaccacg ccgccgtaac tgaagcggac attctcggca aacttgccgc ctaatggccc
     5341 ttggcgggtg agtgccagcc gggtttggcc aaaactgtcc ggctggttgg ccagcacctg
     5401 acgtagctgc gccaggcgct ccagcgggtc ataaccgccg ttgttcaggc gatggcaatc
     5461 ggtccattcg ttgtcaccca ccgcgtagat caatggtttt ttcaggcggt tgaacatggc
     5521 tgcggcgcgg gtaaaggtgg catcatcaca tgacgaactg ccatccttga tgtcgccgtc
     5581 gtacaaagag aaccggatat ccgactgatt gaggctacga atcaccgctt cagtctgcgt
     5641 ggcatcctga ttcttggcgt aaggcatgtc accccaggcg ccaaaactga atactggtgg
     5701 tgccgcctga gccagcgtgg ccgccagcaa gctggccagc aaaagggaaa tgcgcatgag
     5761 agtctcctgt gcgtgcaaaa aagtgtccac catcatacgg caggccagcg acacgccgat
     5821 gacaaactgc ctgccgttgc cctgttcatc ctgagagaat gctttttgca tttttaggtt
     5881 tgtcgagaga ttggcaaatc atgggcaaac caataggtat atttacggta gcccacacat
     5941 tcaaccgctt acggcatccg tgccacttaa tccaattgat taaattggat tgccattccc
     6001 ccggcttgtt gctaccattc ccagcttgcc ggactttttt gcaaggatca tcgtgtgcct
     6061 aaaaaaactg cgctatttgt cgattttgac aatgtatttt tatgtctgaa agccatcgac
     6121 ccgagtttgg cggattgttt tgccaaacgg ccagccgagt ggctgaaaca gttggaaagc
     6181 accgaccgcc gttttctgat tcgtcgctgt tacatgaatc cgtcgtcgtt cgaggaatat
     6241 cgttcttatt ttatttattc tgcgttcgat gtcatcgact gcccacccat caccaaacag
     6301 ggcaagacac tggccgacat gcacatgacc atcgacattg tcgatcgctt gatgcatcca
     6361 acccatttcg aagaattcat gctgttttca gccgactcgg acttcacccc ggtgctgcag
     6421 aaactgcggg aatacgaccg tcagacctgc gtggtgctgg ccggcatgac gtcgggcgcc
     6481 tatcgggcat cagcagatga aatcctgcaa ctggctgagc tgctggaaaa aatgcaaggc
     6541 tatgccggaa atcaggcata tatgcaaaat gagcaactgc aacagtatac tccaacaccg
     6601 cgcacagaat atcccatccg gccggtgcgc ccagcaagct tgccagcacc ggtagccccg
     6661 gaatggttga gtgaagaaaa aaatcaggcg gtgattcagg tattgattga ggcactgcaa
     6721 aatgcctcac atgccattcc ttccgccaag gtgattgaaa tcctgcgtga aaatttcggc
     6781 agtgaggcta ataactggtt tgggtttggt cgcccggcca gtttctttac tgcggtgaat
     6841 atttatgaac acggagtgat tttctcgcag gcggcccctg ggtatcttta cctagccgac
     6901 aagcacacgc ccccgcgtga gctggccacc ctgggcgagt ggttgcgcga cccaggcagc
     6961 gacccggtgc tgatccaatt ggccaaccgg atttgcgcac tgaccgaaac gccgccgtta
     7021 agtccgcagg aataccgtgt ctgcttccag cacattgccg agctcttgca gcaccaaggc
     7081 agtggcctga acgagatgtc caaaaatttg cgcgaccatg ccaaggatca gggtattgcc
     7141 ctgtcacgcc aggacgcgac cggcatgctg ttcctgctgg agcgcaatgg agtcaacctg
     7201 caatatgccg gcaagcaggc cgacgaactg gcagccatct atgccgatgc cgtggcgcgc
     7261 cgttgtgcca ccagccaatg gcagcttagc catgatgaat tgctgctgct gcgcgactgg
     7321 ctgggtgcgc ctgccgaatc aggccaaccc ttgccggcat gaaaaacgcc acgccatgcc
     7381 ttacggggca tggcgtggcg tggg
//
LOCUS       DAOPYX010000141         7391 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_12160_length_7391_cov_12.001499, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000141 DAOPYX010000000
VERSION     DAOPYX010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 7391)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7391)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7391
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12160_length_7391_cov_12.001499"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..98)
                     /locus_tag="PKN55_08325"
     CDS             complement(<1..98)
                     /locus_tag="PKN55_08325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002642527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HNX71290.1"
                     /translation="MSLKCGIVGLPNVGKSTLFNALTKAGIEAANYP"
     gene            complement(128..697)
                     /gene="pth"
                     /locus_tag="PKN55_08330"
     CDS             complement(128..697)
                     /gene="pth"
                     /locus_tag="PKN55_08330"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012695799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HNX71291.1"
                     /translation="MASIRLIVGLGNPGPDYAATRHNAGFWLVDLLARQHSATLRAEG
                     KFFSLYARAGEMHLLEPQTFMNASGKAVAAVARFYKIQPDEILVVHDELDLPPGAAKL
                     KQGGGHGGHNGLRDIIAALGVQDFWRLRLGIGHPGNKAEVVNFVLKPPRKEEQDAIDR
                     AIDCALDVMPAVLAGNMPEAMKDLHTRAK"
     gene            complement(787..1347)
                     /locus_tag="PKN55_08335"
     CDS             complement(787..1347)
                     /locus_tag="PKN55_08335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008952424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HNX71292.1"
                     /translation="MTIEVIAQKRVVEGTGASRRLRRAGKVPGVVYGAGQEAVSIELD
                     HNSLFYAMKDEAFHGAVLSLNLDGETVSVLLRDNQMHPWKPQVLHVDFQRINAAEKIT
                     ARVALHFKNADISPAVKLNGKKVSHIANSVSVRALPADLPAFIEVDLSNLQAGQSIHL
                     SDLVLPANAELADLARGRDLAVAQSS"
     gene            complement(1435..2391)
                     /locus_tag="PKN55_08340"
     CDS             complement(1435..2391)
                     /locus_tag="PKN55_08340"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011137605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HNX71293.1"
                     /translation="MASYDSLMVFTGTANPELAQNVVKHLDISLGRADVNRFSDGEVA
                     IELLENVRGRDVFVLQSTCQPTNDNLMEVLTMVDALKRASAGRITAAIPYFGYARQDR
                     RPRSARVPISAKLVANMLTSAGVDRLLTVDLHADQIQGFFDIPVDNVYATPVLLREIQ
                     TQRYEDLVVVSPDIGGVVRARAMAKALNCDLAIIDKRRPKANVAEVMNIIGEIEGKTC
                     LIVDDMIDTANTLCKAAQALKQKGAARVVAYATHPVLSGQAADRIGASVLDEVVVTDT
                     IPLRADSAAQPKIRVVSIAELIGETLRRINNEESVSYLFVDR"
     gene            complement(2475..2549)
                     /locus_tag="PKN55_08345"
     tRNA            complement(2475..2549)
                     /locus_tag="PKN55_08345"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2515..2517),aa:Gln,seq:ttg)
     gene            complement(2557..3402)
                     /gene="ispE"
                     /locus_tag="PKN55_08350"
     CDS             complement(2557..3402)
                     /gene="ispE"
                     /locus_tag="PKN55_08350"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HNX71294.1"
                     /translation="MALITPFQAFPAPAKLNLTLAITGRRADGYHLLESVFRFIDLMD
                     TVELAVRDDGDIVLDNPIPGVPADSDLTVRAARALQAASGATLGASIRLHKRIPMGGG
                     LGGGSSDAATVLLALNQLWNTQLPRQTLQDIGVKLGADVPVFVFGRPAFATGIGDELT
                     ECAVPDAWYVVLHPGVSVPTAKIFSHQGLTRNSTPSIMRALQTTQRKNDMQSVVCQMF
                     PEVAQCLAKASEYGEPLMTGSGACVFLEFDKEAQAGAVFQSLSSSYAAFIAKGLQHHP
                     LYDGR"
     gene            complement(3393..3950)
                     /gene="lolB"
                     /locus_tag="PKN55_08355"
     CDS             complement(3393..3950)
                     /gene="lolB"
                     /locus_tag="PKN55_08355"
                     /inference="COORDINATES: protein motif:HMM:TIGR00548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein insertase outer membrane protein
                     LolB"
                     /protein_id="HNX71295.1"
                     /translation="MNATRHWSGCIALLGALTLPGCAWLSPPPAPSGAVQALPASWAV
                     AGRLGVKAAGKGSYANFDWKRQGGVDTFDITTPLGQTVARLSRDASGVRLTARGETHQ
                     ADNAEQLTQSLLGWALPLDNLPYWLAGLPAPGQASAPSVDGFEQQGWRIELADFQDTP
                     FGRRPGRVQLSRPDLELRFAMHQWH"
     gene            complement(3947..5662)
                     /locus_tag="PKN55_08360"
     CDS             complement(3947..5662)
                     /locus_tag="PKN55_08360"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNX71296.1"
                     /translation="MNIRLTATAALLASVLSACAITSAPAPVAQAPVPAPEAAYPKLA
                     LSNDILFGVLASEIAAQRGAAAASAPTVLALAQRTRDPRLARRAAEFALTANRLDEAI
                     RALKLWQELEPASDNAQYQLIAALLRAGRLSEATPGVQAQLAARPHEAPQVFMHLAGL
                     LSRQPDKAGAYAMMKTLLAPYPSVAEGHFALMITAQEAGDDAMAQAEMATLATLAPAW
                     DYPVGWQVERLRQTSPEAALSLLERELARRPQAGLELRLSYPRLLVMVKRYDAARDAF
                     VQLSNKYPHQPEVAYAIGVLSYELKDYEEAERSLRDALALGYREPDFVRFTLGQMAEE
                     RKLPQEAVNWYEAITSGPQLPQAQSRLAVLEAEDGKLDAALARLERLADMQNTPRPAR
                     VLAQAQLARNAEQPDRALAILSAALAKDGNVAEWRYERALLLDDRERVAEAERDLRAY
                     LKLKPKSAQGLNALGYILANRTSRIREARELVSRALKLDPDNPMILDSMGWVEYRSGK
                     LEAALRYLQRAHHLMPDPEIAAHLGEVLWKMGREAEARELWARSLEANPDSDIIRRTQ
                     QRFIP"
     gene            5811..6626
                     /gene="mutM"
                     /locus_tag="PKN55_08365"
     CDS             5811..6626
                     /gene="mutM"
                     /locus_tag="PKN55_08365"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HNX71297.1"
                     /translation="MPELPEVETTRRGVAPLLTGRAFTGAVLRQPRLRWPIDPELAER
                     LTGRRVRQVARRAKYLLIELDDGATLIIHLGMSGSLRHLPADTPPERHDHVDLLLGEH
                     CLRYRDPRRFGAILYCPGPVELHPLLVKLGPEPLSDDFDGEVLFAACRGKTAPIKTVL
                     MDNHVVVGVGNIYANEALFAAGVRPTRPAGQVSREECQRLAAAIRATLLRAIEAGGST
                     LRDFTDAIGKPGYFQQHYAVYGRDGLPCHACATLVQHTRLGQRSSFYCPHCQT"
     gene            6640..>7391
                     /locus_tag="PKN55_08370"
     CDS             6640..>7391
                     /locus_tag="PKN55_08370"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNX71298.1"
                     /translation="MTRPTHTVALPVRLWRLTRLSLHVLGGLWQVLTRFQRLPAAQRH
                     AVQRAWSEKLLRTLNIHLRVHGQPPTALYPANTLLVANHVSWLDIFALNSVTLTRFVA
                     KSEIRRWPLIGTLVTRAGTLYIERGNRRDAARINQHMAKAMQDGDCIGLFPEGTTSDG
                     RSLLPFKASLFDAAARAGATVQPVTLRFVNADGSISQAASYVGDTTLLQSIWRLASAR
                     GQVVELHYGQPLSGEQRTRFELCAHAEREIAAG"
BASE COUNT         1227 a         2353 c         2465 g         1346 t
ORIGIN      
        1 ggataattgg cggcttcaat gccggccttg gtcagggcgt tgaacagggt ggatttgccg
       61 acattgggca aaccgacgat accgcatttg aggctcatgg tggtgtcctt gtggattcgg
      121 gcgcgggcta tttggcgcgg gtgtgcaggt ctttcatggc ttcgggcata ttgccggcca
      181 gcacggcggg catgacgtcc agcgcgcagt cgatggcgcg gtcgatggcg tcttgttctt
      241 ccttgcgcgg cggcttgagc acaaaattca ccacttcggc tttattgccg gggtggccga
      301 tgcccagccg cagccgccag aaatcctgca cgcccagcgc ggcgatgatg tcgcgcaggc
      361 cattatgacc gccatggccg ccgccttgct tgagtttggc ggcgccgggc ggcaggtcga
      421 gctcatcgtg cacaaccagg atttcgtcgg gctggatttt gtaaaagcgc gccactgccg
      481 ccacggcctt gccgctggcg ttcatgaagg tttgcggctc cagcaggtgc atctcgccag
      541 cgcgggcgta caggctgaaa aacttgcctt cggcgcgcag ggtggcgctg tgctggcggg
      601 ccagcaggtc aaccagccaa aagccggcgt tgtggcgggt ggcggcgtag tcggggccgg
      661 ggttgcccag gccgacaatc aggcggatag aagccatggt tcatccagaa aaacaaaggc
      721 cggacgacaa aaacggcccg ctgccaggca acgagccgtt ttgacgtccg gattccggtt
      781 cgggaatcag gaagactggg ccacagccag atcgcggccg cgagccagat cggccagctc
      841 ggcgttagcc ggcaggacca ggtcggacaa gtggatggac tgaccggctt gcaggttgct
      901 caggtccact tcgatgaagg ccggcaggtc ggccggcagg gcgcgcacgc tcacgctgtt
      961 ggcgatgtgg ctgaccttct tgccgttcag cttcacggcc gggctgatgt cggcattctt
     1021 gaagtgcagg gcgacgcggg cggtgatctt ctcggcggcg ttgatgcgct ggaagtcaac
     1081 gtgcagcact tgcggcttcc acgggtgcat ttggttgtcg cgcagcagca cggacacggt
     1141 ttcgccgtcc aggttcaggc tcagcacggc gccgtggaaa gcttcgtcct tcatggcgta
     1201 gaacaggctg ttgtggtcca gttcgatgga cacagcttct tggccggcgc cgtacaccac
     1261 gccaggcact ttgccagcgc gacgcaggcg gcggctcgca cccgtaccct cgacaacgcg
     1321 cttctgggca atcacttcga tagtcattgg taactccaaa caggatgtgt gtggcgaagc
     1381 cgcgaccagc ttcgccgggt aatccgccag cagtctggcg ggagattcag caattcagcg
     1441 gtcaacaaac aggtagctga ccgattcctc gttgttgatg cggcgcaggg tttcgccaat
     1501 cagctcggcg atggacacca cgcgaatctt gggctgggcg gccgagtcgg cgcgcagcgg
     1561 aatggtgtcg gtgaccacga cttcgtccag caccgaggcg ccgatgcggt cggcggcctg
     1621 gccagagagc accgggtggg tggcgtaggc caccacgcgg gcggcgccct tttgcttgag
     1681 cgcttgcgcg gccttgcaca gggtgttggc ggtgtcgatc atgtcatcga cgatcagaca
     1741 ggttttgcct tcgatttcgc cgatgatgtt catcacttcg gcgacgttgg ccttgggacg
     1801 gcgcttgtcg atgatggcca ggtcgcagtt gagcgccttg gccatggcgc gggcgcgcac
     1861 cacgccgccg atgtccgggc tgaccaccac gaggtcttcg tagcgttgcg tctggatttc
     1921 acgcagcagc accggggtgg cgtagacgtt gtccaccggg atgtcgaaaa agccctgaat
     1981 ctggtcggcg tgcaggtcaa cggtcagcag gcggtccacg ccggccgagg tcagcatatt
     2041 ggccaccagc ttggcggaaa tcggcacacg ggccgagcgc gggcggcggt cctggcgggc
     2101 gtagccaaag taggggatgg cggcggtgat gcggccggcg gaggcgcgct tgagcgcgtc
     2161 caccatggtc agcacttcca tcaggttgtc gttggtcggc tgacaggtgg attgcagcac
     2221 gaagacatcg cggccacgga cgttttccag cagctcgatg gccacttcac catcgctgaa
     2281 gcggttgacg tcggcgcgtc ccagcgaaat gtccaggtgt ttgaccacgt tctgggcgag
     2341 ttccgggtta gcagtcccgg tgaaaaccat caagctatcg taggaggcca tattccgcta
     2401 cctgactaaa aataaacaaa aagcgtgtaa gtaggctaac ttacacgctt ttgctgccac
     2461 ttaatgtgga aatttggctg gggaggtagg gatcgaacct acgaatgtcg gaatcaaaat
     2521 ccgatgcctt accacttggc gactccccag taactcttac cggccgtcgt acagtgggtg
     2581 atgctgcaac cctttggcga tgaacgccgc ataactgctc gacagcgact ggaaaacggc
     2641 tccggcctga gcttccttat cgaactcgag aaacacacac gctcccgacc cggtcatcag
     2701 tggttcgccg tattcgctgg cttttgccag gcactgcgcc acctcaggaa acatctggca
     2761 aaccacgctt tgcatatcgt tcttgcgctg cgtcgtttgg agagcacgca ttatgctagg
     2821 ggtcgaatta cgtgtcaagc cttggtgcga aaaaattttc gcggtgggca cgctgacgcc
     2881 cggatgcaac accacatacc aggcgtcggg caccgcgcac tcggtgagct cgtcgccaat
     2941 gccggtggcg aaggccgggc ggccaaacac aaacaccggc acgtcagcgc ccagtttcac
     3001 gccgatgtct tgcagggttt gccggggcag ttgggtgttc cacagttggt tcagcgccag
     3061 cagcacggtg gcggcgtccg agctgccgcc gcccaggccg ccgcccatcg ggatgcgctt
     3121 gtgcaggcga atcgaggcgc ccagcgtggc gccgctggcg gcttgcagcg cgcgggcggc
     3181 gcgcacggtc aaatcgctgt cggccggcac gccgggaatg gggttgtcca gcacgatgtc
     3241 gccatcgtcg cgcaccgcca gctcgacggt gtccatcagg tcgataaagc gaaacacgct
     3301 ttccagcaga tggtagccat cggcgcggcg gccggtgatg gccagcgtga ggttaagctt
     3361 ggccggcgca ggaaacgcct gaaacggggt gatcagtgcc attggtgcat cgcaaaacga
     3421 agttccagat caggccggct cagctgcacg cggccaggac ggcggccaaa tggcgtgtcc
     3481 tgaaaatccg ccagctcaat gcgccagccc tgttgttcaa agccatccac gctgggcgcg
     3541 ctggcctggc cgggggcggg caggccagcc agccaatagg gcagattgtc cagcggcaag
     3601 gcccaaccca gcaaagactg ggtgagttgc tcggcgttgt cggcctggtg ggtttcgcca
     3661 cgcgcggtca ggcgcacgcc gctggcgtcg cggctgagcc gcgccacggt ttgccccagc
     3721 ggggtggtga tgtcaaaggt gtccacgccg ccctggcgct tccagtcaaa attggcgtag
     3781 ctgcccttgc cagcggcttt cacgcccagc cggccggcta ctgcccaact ggccggcagc
     3841 gcttgcaccg caccgcttgg tgcgggcggc gggctgagcc aggcgcagcc gggcagggtc
     3901 agcgcgccca gcagggcgat gcagccgctc cagtgtcgtg tggcgttcac gggataaagc
     3961 gctgctgggt gcgccggatg atgtcgctat ccgggttggc ttccaggctg cgtgcccaca
     4021 gttcacgggc ttcggcttcg cggcccatct tccacagcac ctcacccagg tgggcggcga
     4081 tttccgggtc gggcatcagg tggtgagcgc gttgcagata gcgcagcgcg gcttccagct
     4141 tgccggagcg gtattccacc cagcccatgc tgtccagaat catcgggttg tccgggtcca
     4201 gcttcagcgc gcggctgacc agctcgcgcg cctcgcggat gcggctggtg cggttggcca
     4261 ggatatagcc cagcgcgttc agcccttgcg cgctcttggg tttgagcttg aggtaggcgc
     4321 gcagatcgcg ctcggcctcg gccacccgtt cgcggtcgtc cagcagcagg gcgcgctcgt
     4381 agcgccattc ggcgacattg ccatccttgg ccagggcggc gctgaggatg gccagcgcgc
     4441 ggtcgggttg ctcggcattg cgcgccagtt gcgcttgcgc cagcacgcgc gccgggcgcg
     4501 gggtgttttg catgtcggcc aggcgctcca gccgggccag cgcggcgtcc agcttgccat
     4561 cttcggcttc cagcaccgcc agccggcttt gtgcctgcgg cagttgcggg ccgctggtga
     4621 tggcttcgta ccagttgacg gcttcttgcg gcagtttgcg ctcttccgcc atctggccca
     4681 gggtgaagcg tacaaaatcc ggctcgcggt agcccagcgc cagcgcgtcg cgcagcgaac
     4741 gttcggcttc ttcgtagtct ttcagctcgt aagacaatac gccaatggcg taggccactt
     4801 cgggctgatg ggggtatttg ttgctcagtt gcacaaaggc gtcgcgggcg gcgtcgtagc
     4861 gcttgaccat caccagcagg cgcggatagc tcagccgcag ttccagcccg gcttgcggcc
     4921 ggcgagccag ttcgcgctcc agcaggctca gtgcagcctc gggcgaggtt tgccgcagac
     4981 gctcgacttg ccagcccacc ggataatccc aggctggggc cagggtggcc agggtggcca
     5041 tctcggcttg cgccatggcg tcgtcaccgg cttcctgagc ggtgatcatc agggcaaaat
     5101 ggccctcggc cacgctggga tacggcgcca gcagggtttt catcatggcg taggcgccag
     5161 ctttgtctgg ttgccgcgac aagagtccag ccagatgcat gaacacctgc ggggcttcat
     5221 gcgggcgggc ggccagttgc gcttgtacgc ccggcgtggc ttcgctcagc cggccggcgc
     5281 gcagcagcgc ggcaatcagc tggtattggg cgttgtccga ggcgggctcc agctcttgcc
     5341 acagcttgag cgcgcggatg gcctcgtcca gccggttggc ggtcagggca aattcggcgg
     5401 cgcggcgcgc caggcgtggg tcgcgggtgc gttgcgccag cgccagcacg gtaggcgctg
     5461 atgcggcggc cgcgccgcgt tgggcggcaa tttcgctggc cagcacgcca aacaaaatat
     5521 cattgctcag cgcaagcttg ggataggcgg cttccggcgc ggggaccggc gcctgggcca
     5581 cgggagccgg cgccgaggtg atggcgcagg ccgacagcac ggatgccagc agcgcggccg
     5641 tggcggtaag gcggatattc atgaatgatt tcgcgtaggg ctgaccattt aaggacgccc
     5701 ggacgggcag ggccgcatcc aggccggctg cgcgctcagg cattgacgct gagcggcgcg
     5761 atgtctcgcg ttagaataac acgcccaact ttagccatgg ccgcttaaac atgcctgaat
     5821 tgccagaagt ggaaaccacc cggcgcggcg tcgccccctt gctcaccggc cgcgccttca
     5881 ccggcgcggt gctgcgccag ccgcgcctgc gctggccaat cgaccccgag ctggccgagc
     5941 gcctgactgg ccgccgcgtg cgccaggtgg cgcgccgcgc caaatacctg ctgatcgagc
     6001 tggacgacgg cgccacgctg atcatccatc tgggcatgtc cggcagcctg cgccatctgc
     6061 cggccgacac cccgcccgag cgccacgacc atgtggattt gctgctgggc gagcactgcc
     6121 tgcgctaccg cgacccgcgt cgctttggcg ccatcttgta ctgcccgggc ccggttgaac
     6181 tgcatccgct cttggtgaaa ctggggccgg agcccttgtc tgatgatttt gacggcgagg
     6241 tgttgtttgc cgcctgccgg ggcaaaaccg cgccaatcaa aaccgtgctg atggacaatc
     6301 atgtggtggt gggggtgggc aatatttacg ccaacgaggc gctgtttgcc gctggcgtgc
     6361 gccccacccg ccccgccggg caggtcagcc gcgaagaatg ccagcgcctg gccgcggcca
     6421 tccgcgccac cctgctgcgc gccatcgagg ccggcggcag cacgctgcgc gactttaccg
     6481 acgccattgg caagccgggg tatttccagc aacactacgc cgtgtatggc cgtgacggcc
     6541 tgccctgtca tgcctgtgca accttggtgc aacacactcg cctggggcag cgctccagtt
     6601 tttactgccc gcattgtcaa acctgacccc aacgccatca tgacccgtcc cacccacaca
     6661 gttgccctgc ccgtccgcct gtggcggctc acccgcttaa gcctgcatgt gctgggcggg
     6721 ctgtggcagg tgctcacccg ctttcagcgc ctgccggcgg cgcaacgcca cgccgtgcaa
     6781 cgcgcctggt cagaaaaact gctgcgcacg ctgaacatcc acctgcgcgt gcacggccag
     6841 ccgcccaccg cgctctaccc ggccaacacc ctgctggtgg ccaatcatgt gtcctggctg
     6901 gatatttttg ctctgaacag cgttaccctc acccgttttg tcgccaaaag cgaaatccgc
     6961 cgctggccgc tgatcggcac cctggtcacc cgcgccggca cgctttatat cgagcgcggc
     7021 aaccgccgcg acgccgcccg catcaaccag cacatggcca aggccatgca ggacggcgac
     7081 tgcatcgggc tattcccgga aggcaccacc tccgacggac gcagcctgct gccattcaaa
     7141 gcctcgctgt tcgacgccgc cgcccgcgcc ggcgccaccg tgcagccggt gacactgcgc
     7201 tttgtcaacg ccgacggcag catcagccag gccgccagct atgtcggcga caccaccctc
     7261 ttgcaatcca tctggcgact ggcctcggcg cgcggccaag tggttgaact gcactacggc
     7321 cagccgctca gcggcgagca acgcacccgc tttgagctgt gcgcgcatgc ggaacgggag
     7381 attgcggcgg g
//
LOCUS       DAOPYX010000142         7378 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_12194_length_7378_cov_9.767445, whole genome shotgun sequence.
ACCESSION   DAOPYX010000142 DAOPYX010000000
VERSION     DAOPYX010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 7378)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7378)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7378
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12194_length_7378_cov_9.767445"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(116..883)
                     /locus_tag="PKN55_08375"
     CDS             complement(116..883)
                     /locus_tag="PKN55_08375"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNX71299.1"
                     /translation="MSPLLADLAGKSFLITGASSGIGAATARLLGRAGAQLTLTARRA
                     DLLENVAAEVRAAGGQAWACPADITVEAEHARLVDAAVAQFGRLDGAFNNAGVLGRAG
                     PLHTLSTADFAEVMLANVQAMFWAMKAQIPVLRAQGGGAIVNTASVVAQVGFANSAIY
                     TASKHAVLGLTRSAALENFAHGVRINAVNPGPIVTPMAEVGFGGVEAMRAFLQATPAG
                     RPGEPEEVAGLVAFLLSAAASYVSGQGVTVDGGFTVA"
     gene            988..1884
                     /locus_tag="PKN55_08380"
     CDS             988..1884
                     /locus_tag="PKN55_08380"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HNX71300.1"
                     /translation="MSAASLNDLHAFATVARLRSFRQAAAVLGVSPSALSHALRGLES
                     RLGVRLLNRTTRSVAPTEAGARLLARLSPALGDIQAALDEVNDFRDSPLGRLRINAPR
                     PACEQVLAPLVCRFLARHPGMQVELVADDALVDIVAAGFDAGVRFGERLQQDMVALPI
                     GPAQRFVVVASPAYLAAHGAPRTPHELPGHACVRLRFPSGAIYRWDFARDGEALEVEV
                     NGPITVGEMPLAIHAAEAGLGLAYVYASYAAAGLAAGRLRTVLDDWRPADEHFYLYYP
                     SRKLLPAGLRAFVDMLHEHPPR"
     gene            2008..5871
                     /locus_tag="PKN55_08385"
     CDS             2008..5871
                     /locus_tag="PKN55_08385"
                     /inference="COORDINATES: protein motif:HMM:NF016260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HNX71301.1"
                     /translation="MRSTHAPMTAATPPDAPELTSSSAVPPKPRRRWPWALAVVCLLP
                     LLLLAALGSEAGLRAALAAAQSLSGGAVQVATLDGSLASGNLRLRGVLARTALSEYQL
                     DELALRWRPAALWRGRLDIDALELGVLRLRTLAPDPTPPSLPADLRLPLALHIERLRL
                     ARLEVGSVALGPLLAKVDSDGEQHRLWVSEARTPWGEGHGEFTLAGAQPFALSGVWRH
                     GGQLDGAPINGEARLSGDLRALQLALALGYQHSALGARATLAPFAAQPFARLRQAEAT
                     LVRLNPAQWLPGAPEADISLSVASAPVSAARQRLRLRADNALAGDWAAKRLPWQDGDA
                     ELDIDADALTLRQLTVHALDGEFTGSGHWRPEAFSMDGVLRGVRLQRLAPALPDWPAD
                     GAVQLRGRPDAPQLSVQLNDRQQRELRAELALAGVGAARKLLLNTLQLRDGPARLDGA
                     GELALAGERAFRLQATLRQLNPARWSPQLDAGQLNAALSVQGAAQPLRVQGELTLADS
                     QYRGQPASARLRGQWDGQRAHGLSLDAQLGGNQLHADGALGGERDSLRFTLAAPRLEQ
                     LGSGFAGELQGQGQLLGQGWRLRLNGELRARQLRAPGGVSVDSLQLQARLPDDFNLPG
                     EATLEARQLRAAGWQISSAQARYQGQRARHTLSASARGDGPQGEFDVQAALEGGWQNG
                     QGWQGRVLKLSNQGRLPLALDAPASVQVQDAQRWRLSGARLRALGARLNIELLARRGA
                     LWSSQGQLSAVSPSEWLKKFNLASPLDGPLLLAADWRVQDSQGRLTLRREAGDVWPAG
                     QSARPLRLEQAQLQFTVSPQRWQAQAQLRSAHLGRLDANGDWRPAANGLPNSQSPLRL
                     AVNADAPDLAAWATWLPPGMRVGGRLAANVSLEGSLATPRWRGALKGEALALSRPADG
                     LALENGQLRASLQDELVKLDMLSLRDRQGGELSLSGQANWRRPDSAQFTLNVRKLAAL
                     SHPSRSLSVSGQAQLRQQGSGLLLSGGFTVDQGRITLPDSDTPSLGDDVVIVGRPRAE
                     PDAPLAIPVAVALDLDLGEQFRLSGKGLDVKLAGRLRLAADAGQAPRATGAVRVVSGH
                     YAAYGQRLDISRGVLTFVRRLDDPGLDVLAVRKGLSVEPGVEISGTAQAPRVQLVSTP
                     ELPDSEKLSWLVLGRSATALRGGETDLLFSAASTLLGSSGALGIQQQLAARLGLDELS
                     VGAASNRPTVQSSKTVASRNPLDNQVVTLGKRLASGLYLGYEQSLTGVGQAVKLTWEW
                     SRQWSMVLRAGEQSALDVVYGRGFD"
     gene            6348..6494
                     /locus_tag="PKN55_08390"
                     /pseudo
     CDS             6348..6494
                     /locus_tag="PKN55_08390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688245.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
     gene            complement(6803..7312)
                     /locus_tag="PKN55_08395"
     CDS             complement(6803..7312)
                     /locus_tag="PKN55_08395"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HNX71302.1"
                     /translation="MNADELKARQAPLKAAYQQDPACACLTLSASGELAPGEQTVTIR
                     TPHTTLRAGLHPAAGGSGEDACSGEMLLEALIACAGVTLQAVATAMGIALRGGRVRAE
                     GELDFRGTLGVSKEAPVGFAAIRLTFELDTDASDEQLTTLLRLSERYCVIYQSLKQPP
                     ELSWVRLPG"
BASE COUNT         1076 a         2518 c         2585 g         1199 t
ORIGIN      
        1 cctttctttt cccccttttc tttggcgaga caaagaaaag gggtcgggtg cggggggaac
       61 ccccgcccaa tattcagccc cagcctaccc gcgcgtcagc gcggcatcat cacaatcacg
      121 ccaccgtaaa ccccccatcc accgtcaccc cctgcccact cacataactg gccgccgccg
      181 acaacaaaaa cgccaccaaa cccgccacct cctccggctc acccggccgc ccggccggcg
      241 tcgcctgcaa aaacgcccgc atcgcctcca ccccgccaaa ccccacctcc gccatcggcg
      301 tcacaatcgg ccccggattc accgcattga tgcgcacccc atgggcaaaa ttctccagcg
      361 ccgcactgcg cgtcagcccc agcaccgcat gcttgctggc ggtgtaaatc gcgctattgg
      421 caaaccccac ctgcgccacc accgacgccg tgttgacaat cgccccgccg ccctgggcgc
      481 gcaacaccgg aatctgcgcc ttcatcgccc agaacatcgc ctgcacattg gccagcatca
      541 cctcggcaaa atccgccgtg ctcaaggtgt gcaaggggcc ggcgcggccc agcacgccgg
      601 cattgttaaa cgcgccatcc agccggccaa actgggcgac ggcggcgtcc accaggcgcg
      661 cgtgttcggc ttccacggtg atgtcggccg ggcatgccca ggcctggccg cctgccgcgc
      721 gcacctcggc ggccacgttt tccagcaggt cggcgcgccg cgccgtcagg gtcagctgcg
      781 cgcccgcacg gcccaagagc cgcgcggtgg cggcgccaat gccagacgac gcgccagtga
      841 tcaaaaagct cttgccagcc aggtcagcca aaagagggga catgtgatgc gctcctgtgt
      901 ggggaaaatc gttcacacag gctaagcgtg cgccgcttgt gcaacaatcc atgctggatc
      961 gcatggctta ttgaggaaaa ttcagcaatg tccgccgcca gcctgaatga tttacacgct
     1021 tttgccaccg tggcccgtct gcgcagtttt cgccaggccg ccgccgtgct cggggtgtcg
     1081 ccctcggcct tgagccacgc cttgcgcggt ttggagtcgc ggctgggcgt gcgcctgctc
     1141 aaccgcacca cgcgcagcgt ggcgcccacc gaggccggcg cgcgcttgct ggcgcggctc
     1201 agcccggcgc tgggcgatat tcaggccgcg ctggacgagg tcaacgattt tcgcgattcg
     1261 cccttgggcc gcttgcgcat caatgcgccg cgcccggcct gcgagcaggt gctggcgccg
     1321 ctggtgtgcc gctttttggc ccgccatccc ggcatgcagg tggagctggt ggcggacgat
     1381 gcgctggtgg atattgtcgc ggcggggttt gacgctggcg tgcgttttgg cgagcgcctg
     1441 caacaagaca tggtggcgct gcccatcggc ccggcgcagc gctttgtggt ggtggcctcg
     1501 ccggcctatc tggccgcgca tggcgcgccc cgcacgccgc atgaactgcc gggccatgcc
     1561 tgcgtgcggc tgcgctttcc cagcggggcg atttaccgct gggattttgc ccgcgacggc
     1621 gaagcgctgg aggtggaggt gaacgggccg atcaccgtgg gcgaaatgcc gctggccatc
     1681 cacgccgccg aagccggcct gggcctggcc tatgtgtacg ccagctacgc cgccgccggg
     1741 ctggctgctg ggcgtttgcg caccgtgctg gacgattggc gacctgcgga cgagcatttt
     1801 tatctgtatt accccagccg caaattattg cctgctggct tgcgggcgtt tgtggacatg
     1861 ctgcatgaac atccgccgcg ctgagcgccg cccaccaagc tcagcgcggc gctcctggca
     1921 aggccgcgca acgacgccag gggggttggt gagcagcgcc aagcttgcca gccatgccag
     1981 ccgggtgcga acatcggcgc attcctcttg cgttctaccc acgcgcccat gaccgccgcc
     2041 accccgcctg acgcccccga attgacttcc tcttctgctg tgccgcctaa gccgcgtcgt
     2101 cgttggccct gggcgctggc cgtggtgtgc ctgttgccct tgctgctgct ggccgcgctg
     2161 ggcagcgaag ccggcttgcg cgcggcgctg gcggcggcgc aatcactgag cggcggcgct
     2221 gtgcaggtgg cgacgctgga cggcagcctg gccagcggca acttgcgctt gcgcggggtg
     2281 ctggcgcgca cggccttgag cgaatatcag ctggacgaac tggccctgcg ctggcggcca
     2341 gccgcgctgt ggcgcggtcg gctggacatt gacgccctgg agctgggcgt gctgcgcctg
     2401 cgcaccctgg cccccgaccc caccccaccc agcttgcccg ccgacctgcg cctgcctctg
     2461 gcgctgcata tcgagcgcct gcgcctggcg cggctggagg tcggcagcgt ggcgctgggg
     2521 ccgctgctgg ccaaggtgga tagcgacggc gagcagcatc ggctgtgggt cagcgaggcg
     2581 cgcacgccgt ggggcgaggg ccatggcgaa ttcaccctgg ccggcgcgca gccgtttgcg
     2641 ctgtccgggg tatggcggca tggcggccag ctggacggcg cgccaatcaa tggtgaagcg
     2701 cgcttgagtg gggatttgcg cgccttgcag ctggcgctgg ccttgggtta tcagcacagc
     2761 gccttgggcg cgcgcgccac cttggcgccg tttgccgccc agccctttgc ccgcctgcgc
     2821 caggccgaag ccacgctggt gcggctgaat ccggcgcaat ggctgcccgg cgcgcccgag
     2881 gcggatattt ccttgagcgt ggccagcgcg ccggtgtcgg ctgcgcgtca gcgcttgcgg
     2941 ctgcgcgccg acaatgcctt ggccggcgat tgggcggcca agcgcctgcc ttggcaggat
     3001 ggcgacgccg agctggacat cgacgccgac gcgctgacgc tgcgccagct gaccgtgcat
     3061 gcgctggatg gcgaattcac tggcagcggc cattggcggc ccgaggcctt cagcatggac
     3121 ggcgtgttgc gcggcgtgcg cttgcagcgc ttggccccgg cgctgccgga ctggccggcc
     3181 gatggcgcgg tgcagctgcg cggccggcca gacgcgccgc aattgagcgt gcagctgaac
     3241 gaccgccagc agcgcgagtt gcgcgccgag ctggcgctgg ccggcgtcgg ggcggcgcgc
     3301 aagctgctat tgaacacctt gcaattgcgc gatggccccg cccggctgga cggcgctggc
     3361 gagctggcct tggccggcga gcgggcgttt cggctgcagg ccacgctgcg ccagctgaat
     3421 ccggcgcgct ggtcgccgca gctggacgcc ggtcagctca acgccgcctt gagcgtgcaa
     3481 ggcgcggcgc agcccttgcg cgtgcagggc gaactgacgc tggccgacag tcagtatcgc
     3541 ggccagccgg cctcggcgcg cttgcgcggc cagtgggacg gccagcgcgc gcatggcctg
     3601 agcctggacg cccaactggg cggcaaccag ctgcacgccg acggcgcgct gggcggcgag
     3661 cgcgacagcc tgcgctttac cctggccgcg ccccggctgg aacagctggg cagcggcttt
     3721 gccggcgagc tgcaaggcca gggccagcta ttgggccagg gctggcggct gcgcctgaat
     3781 ggcgaattgc gcgcccgcca actgcgcgcg cccggcggcg tcagcgtgga cagcctgcaa
     3841 ctgcaagccc ggctgccgga tgatttcaac ctgcctggcg aagccacgct ggaggcgcgg
     3901 caactgcgcg ccgccggctg gcaaatcagc agcgcgcagg cgcgctacca gggccagcgc
     3961 gcgcgccaca cgctgagcgc cagcgcgcgc ggcgatggcc cgcagggcga atttgatgtg
     4021 caagccgcgc tggaaggcgg ctggcaaaac ggccagggct ggcaaggccg ggtgctgaaa
     4081 ctgagcaatc aaggccgttt gccgctggcg ctggacgcgc cggccagcgt gcaagtgcag
     4141 gacgcgcaac gctggcggct gagcggcgcg cgcctgcgcg ccttgggcgc gcggctgaat
     4201 atcgagctgc tggcgcggcg tggcgcgctg tggagcagcc agggccagct cagcgcggtg
     4261 tcgccaagcg agtggctgaa aaaatttaac ctggccagcc cgctggacgg cccgctgctg
     4321 ctggccgccg actggcgggt gcaagacagc cagggccggc tgacgctgcg ccgcgaagcc
     4381 ggcgatgtct ggccggcagg ccagagcgcg cggcccttgc ggctggagca ggcgcaactg
     4441 caattcaccg tctcgccgca gcgctggcag gcgcaggccc aactgcgcag cgcccacctg
     4501 ggccggctgg acgccaatgg cgactggcgg ccggcggcca atggtttgcc caattcgcaa
     4561 agcccgctac ggttggccgt caatgctgac gcgccagacc tggccgcctg ggccacctgg
     4621 ctgccgccgg gcatgcgcgt gggcggacgg ctggccgcca atgtcagcct ggagggcagc
     4681 ctggccacgc cgcgctggcg cggcgcgctc aagggcgagg cgctggcgct cagccggccg
     4741 gccgacggcc tggcgctgga aaacggccaa ctgcgcgcca gcctgcaaga cgagctggtc
     4801 aagctggaca tgctcagcct gcgcgaccgc cagggcggcg agctgagcct cagcggccag
     4861 gccaactggc gacgcccgga cagcgcgcag ttcaccctga acgtgcgcaa gctggcggcg
     4921 ctcagccacc ccagccgcag cctgagcgtc agcggtcagg cgcagttgcg ccagcagggc
     4981 agcggcttgt tattgtctgg cggctttacc gtcgatcagg gccgcatcac cctgccggac
     5041 agcgacacgc ccagcctggg cgacgatgtg gtgattgtcg gccggccgcg cgccgagcca
     5101 gacgcgccgc tggccatccc ggtggcggtg gcgctggatc tggacctggg cgagcaattc
     5161 cgtctgagcg gcaagggcct ggacgtcaaa ctggccggcc gcctgcgcct agccgccgac
     5221 gccggccagg cgccgcgcgc cacgggggcg gtgcgggtgg tcagcggcca ttacgccgcc
     5281 tatggccagc gcctggacat cagccggggc gtgctgacct ttgtccgccg cctggacgac
     5341 cccggcctgg acgtgctggc ggtgcgcaag ggcctgagtg tggagccggg ggtggaaatc
     5401 tccggcaccg cccaggcgcc gcgcgtgcaa ttggtgtcca cgcccgagct gccggatagc
     5461 gaaaaactct cctggctggt gctggggcgc agcgctaccg ccttgcgcgg cggcgaaacc
     5521 gatttgctgt tttcggctgc cagcacgctg ttgggcagct ccggcgcgct gggcatccag
     5581 cagcaactgg ctgcgcgcct ggggctggat gagctgagcg tcggcgccgc cagcaaccgg
     5641 ccaaccgtgc aaagcagcaa aaccgtggcc agccgcaatc cgctcgacaa tcaagtggtg
     5701 accctgggca agcgcctggc tagcggcctg tatctgggct acgagcaaag cctgaccggc
     5761 gtgggccagg cggtgaagct gacttgggaa tggtcgcggc agtggtcgat ggtgctgcgc
     5821 gctggcgagc aaagcgcgtt ggatgtggtg tatgggcgcg gatttgactg aacagcgctg
     5881 attcggtgcg ccgctggccg tgcttgaggc cgcaccccag tcaaagccgc tacgcggcat
     5941 gggcttgcat ggtgctgtca agtgcgtggc gatcgtgctg tgtgatatgg gttagcgccg
     6001 gatttgcctg gggcggatat gtggaaaaac ctgcccatcg ccaaggcggc tgcgctacca
     6061 tccacagtaa gagctgctca caatcccctg gcgttgttgc acgaccttgc cgtgtctgcc
     6121 tggccgggcg tgttgggctg agttttgtga gtggcctaag cattgcctgt caggccgtgc
     6181 gccaagtttc gttcatctcg tccttcgggc gggcgtgtgt cgccagtcag tctgtggcga
     6241 catgtcttgt gatgagtgag ctgtgcagag ccgaccaaga tcggccaggc gtgtggcggg
     6301 gcggagtggc ccactggcag gcttctgatg atgaccggga gactatcatg cgcgtgctgc
     6361 tcgccatcct gttgccgtgt accgcgtttt tccgtctgcg ccgcactggg gcaggcgtat
     6421 tcagcctgtt gttgcaactg agtgtggtgg gctggctgcc cgccgcgttc tgggcggtat
     6481 atgcgctggg gcgagccagc gccagccatg gccacgcccg tcaccatggc caccatgcca
     6541 cgccgcatcc accggtggcg cgtcccacac cgccggcccc gccgtccatg cccagcccgg
     6601 ccgggcgtgg cgccgtcagc gccaaaatgc cgcgtcgccg ctagtcacgc cttgcaaaga
     6661 gccacttgcc acgtccaggc gggggttttg tctgcggcgc ttaaccttgc tgacaaaacc
     6721 ccctggcgtt gtagtgtgct ttccagactg tctgcgatca tccgctgggt cagggcggct
     6781 tggtgagttt tgtcagcgat ttttaacccg gcaggcgcac ccaggacagt tctggcggct
     6841 gtttcagcga ctgataaatc acgcagtagc gctcggacag ccgcagcagg gtggtcagtt
     6901 gctcgtcgct ggcgtcggta tccagttcaa atgtcaggcg gatggcggca aagcccaccg
     6961 gggcttcctt gctgaccccc agtgtgccgc gaaaatccag ttctccctcg gcgcgtaccc
     7021 gtccaccgcg caacgcaatg cccatggccg tggccacggc ctgcaaggtg acgccagcgc
     7081 aggcaatcag cgcttccagc aacatttcgc ccgagcaggc gtcttcgccg ctgccgcctg
     7141 cggccggatg caagccagcc cgcagggtgg tgtgcggcgt gcggatggtg acggtctgct
     7201 cgcctggggc cagctcgccg ctggcggaca gggtcaggca ggcgcaggcg gggtcttgct
     7261 ggtaagccgc tttcagcggc gcctggcggg ctttcagttc atctgcattc atggtgaggc
     7321 tcctcgttga cgtccgtcgg gatgcggact gaaccagcat ggcagcatgt gtcagggc
//
LOCUS       DAOPYX010000143         7309 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_12358_length_7309_cov_10.350014, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000143 DAOPYX010000000
VERSION     DAOPYX010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 7309)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7309)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7309
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12358_length_7309_cov_10.350014"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..231
                     /gene="glnA"
                     /locus_tag="PKN55_08400"
     CDS             <1..231
                     /gene="glnA"
                     /locus_tag="PKN55_08400"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245865.1"
                     /note="forms a homododecamer; forms glutamine from ammonia
                     and glutamate with the conversion of ATP to ADP and
                     phosphate; also functions in the assimilation of ammonia;
                     highly regulated protein controlled by the
                     addition/removal of adenylyl groups by adenylyltransferase
                     from specific tyrosine residues; addition of adenylyl
                     groups results in inactivation of the enzyme; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HNX71303.1"
                     /translation="DKNLYDLPPEEDKLIPTVCASLEEALAALDADREFLTRGGVFSN
                     DFIDAYIELKMDEVNKTRMTAHPVEFQMYYSL"
     gene            353..889
                     /locus_tag="PKN55_08405"
     CDS             353..889
                     /locus_tag="PKN55_08405"
                     /inference="COORDINATES: protein motif:HMM:NF024901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HNX71304.1"
                     /translation="MTYSRLLICLMCALPAMAGANAPIYKYVDPYGNVTYSNTPVPGA
                     KRSALPPVAINPNIASSAVLSNRDSKQVVNVRPEPASSASAARAGNGGASGSAPAAGQ
                     YPVIDGATQRNRDASRTRILQDELDNEQRALANARRMLQETERYAKPDSALLRGLRDS
                     VLEREKNIAALQREIGRR"
     gene            1295..2350
                     /gene="glnL"
                     /locus_tag="PKN55_08410"
     CDS             1295..2350
                     /gene="glnL"
                     /locus_tag="PKN55_08410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011137138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HNX71305.1"
                     /translation="MPDTAFAGLELLETATLAVCRDGGIRYVNPACENLLALSQKELL
                     RHTLSSLFVDSRALVAGLRAAVTHDVSFTEHDLSLNTINQISLQVICTLTPINVAGCA
                     ALIELRPIEQQLKIANEERLLIQQAANRELIRNLAHEIKNPLGGIRGAAQLLAHELAD
                     RPDLKEYLDVITHEAVRLQQLLDRLLAPNRKHILGEVNIHEVLERVRSVALAEYPQGI
                     AIRRDYDTSLPALTADKEQLIQVVLNIVRNAIQAMHGRGQIVLRTRVARQVTLARKRY
                     QLAMLLQIIDNGPGIPAELKDHIFYPLVTGRAEGTGLGLTIAQTYVHQHGGTVEFDSR
                     PGHTCFTVLLPFGDKKA"
     gene            2347..3771
                     /gene="ntrC"
                     /locus_tag="PKN55_08415"
     CDS             2347..3771
                     /gene="ntrC"
                     /locus_tag="PKN55_08415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018150674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HNX71306.1"
                     /translation="MTPSVWIIDDDKSIRWVLEKALTRAGISYASFTNADDALNALYD
                     TLPKVILSDIRMPGTDGLKFLATVKAEHPTLPVIVMTAHSDLDSAVAAFQGGAFEYLP
                     KPFDVDQAVQLVERALGEAERDNAGPDAMDATPVMLGQAPAMQDVFRAIGRLSQSSVT
                     VLITGESGSGKELVAHALHRHSNRAAKAFIAINTAAIPRDLLESELFGHERGSFTGAT
                     TQRRGRFEEAEGGTLFLDEIGDMPTELQTRLLRVLSDGFFYRVGGHQPIKANVRVIAA
                     THQNLEDRVKQGLFREDLFHRLNVIRLRLPPLRERQEDVPLLARHFLFKSAQQLGVEP
                     KRLSDGAMDVLKTYPFPGNVRQLENICHWLTVMAPGQTIEPGDLPPEMKETPLSAPVG
                     VVGGDWVSALEHLAEQRLKRGEVGIIDSLTHQFEAALIKTALAHTGGRRVEAAHLLGL
                     GRNTLTRKIQELGIDGGRDDEAGV"
     gene            4062..4889
                     /gene="pabC"
                     /locus_tag="PKN55_08420"
     CDS             4062..4889
                     /gene="pabC"
                     /locus_tag="PKN55_08420"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008696 - 4-amino-4-deoxychorismate lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HNX71307.1"
                     /translation="MSIWINGQPATQLDASDRGLSYGDGLFRTLRLLDGAPLAWRWHW
                     ARLQHDCAALRLPCPEAELLLAELAAVSAEWPDAVAKLTLTRGVGPRGYAPPAAPQPT
                     RIVQAGPRVAPPAERYSQGVAVYACALRLGLQPALAGVKHLNRLENVLARAEWQGDEY
                     AEGLLCDSEGWVIEGTMSNLWIRRGRELLTPQLDRCGVSGAARAWVFDTAPKLGYQVR
                     EWRLRPADVLAADEAMLCNSLIGFWPIARYQQRRWSVFTASEALNSAWLAPESAISG"
     gene            4984..5748
                     /locus_tag="PKN55_08425"
     CDS             4984..5748
                     /locus_tag="PKN55_08425"
                     /inference="COORDINATES: protein motif:HMM:NF012995.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arylamine N-acetyltransferase"
                     /protein_id="HNX71308.1"
                     /translation="MHASNFVLDDYLSRIGYHGPRDASAATLAQLMRAQLFTVPFENL
                     DVQAGKIVSMTPEDIVQKIVYQPRGGYCYEVNGLFAMALQALGFSYTFLAARPMFYPV
                     RRPKTHMVLCVRADGRDWLCDLGFGSYGIRAPLALDALDSDIAQDHDRFRLSRDAQGV
                     YLLQAWVDGEWANQYGFDLHPQEWIDFAPANYLNSTHPEAIFVQKLLVVRHTPRGRHI
                     LLGERLKTIEDGVSQLRELGAAEVAVVLREVFALAA"
     gene            complement(5762..7099)
                     /locus_tag="PKN55_08430"
     CDS             complement(5762..7099)
                     /locus_tag="PKN55_08430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003746337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HNX71309.1"
                     /translation="MSDLFSASSPAELFAQTPAAPLAEALRPHSLDEVIGQRHLLGPG
                     KPLQLAFQSGKPHSMIFWGPPGVGKTTLARLTASAFHCEFIALSAVFSGVKDIRAAMD
                     TAQRHLARGVRTILFVDEIHRFNKSQQDALLPYTESGLVTFIGATTENPSFEVNAALL
                     SRAQVYVLKSLGDDELRLLLTRAQRKALPQLRFDEAAQAALVGHADGDARRLLNLLEQ
                     CAAAAQAAQVEAIDAQFVDSALTVNSRRFDKGGDHFYDQISALHKSVRGSHPDAALYW
                     LTRMLDGGADPRYLARRIVRMAWEDIGLADPRAMQIANDAALTYERLGSPEGELALGQ
                     AVIYLAIAAKSNAGYTAYNAARAFVKQDKSREVPVHLRNAPTQLMKELGHGHAYRYAH
                     DEPHGYAAGERYLPEGMAEPGWYQPVPRGLESKIADKLAFLRGLDAEADSDTH"
BASE COUNT         1294 a         2387 c         2297 g         1331 t
ORIGIN      
        1 gacaagaacc tgtacgatct gccgccggaa gaagacaagc tgatcccgac cgtgtgcgcc
       61 agcctggaag aagccctggc cgccctggac gccgatcgcg agttcctgac ccgtggcggt
      121 gtgttcagca acgacttcat cgacgcttac atcgagctga agatggacga agtgaacaag
      181 acccggatga ccgcccaccc ggtggaattc cagatgtact acagcctgta atccgccttc
      241 tggcgcgatt gtctggccaa aacccccgca tggcttgccg tgcgggggtt tttcatggcg
      301 gggccggtgc ggcggtttcc tgtatgccaa caaggcgtta tcatgtttgc ccatgactta
      361 ctcgcgcctg ttgatctgcc tgatgtgtgc cctgcccgcc atggcggggg ccaatgcgcc
      421 catttacaaa tacgtcgatc cctacggcaa cgtcacctat tccaacaccc cggtgcctgg
      481 cgccaagcgc agcgcattgc cgccggtggc catcaacccg aacatcgcat cttctgcggt
      541 gttgtccaac cgcgacagca agcaagtggt gaatgtgcgc cctgagccgg cttcgtcagc
      601 ttctgctgca cgggccggca acggcggcgc cagcggcagc gcgcccgctg ccggccaata
      661 cccggtgatc gacggcgcaa cccagcgcaa ccgcgacgcc agccggacac gcatcctgca
      721 agacgaactg gacaacgagc agcgcgcgct ggccaacgcc cggcgcatgc tgcaagaaac
      781 cgaacgctac gccaaacccg attcggcact gttgcgcggc ctgcgcgaca gcgtgctgga
      841 gcgcgaaaaa aatattgccg ccctgcaacg ggaaatcggc cgccgctaac ccacccctgc
      901 ggagtcgatg gcgtggtgag cgccccaacc ccctgattcc cggcgttgct gtcatgcctg
      961 tcctgtctgc ggggatttcg ccaggattgc tgtgcggcgt tgagtgggcg acgctttgtt
     1021 tgttgatttt tttaaacgca cagtcggtgt gcgctgcctt gtcatggtgc gcaccgtggc
     1081 gcatgcgcga ggccatgcgc cattctctgg ccatttttgg cgaaaaatcc gatattttgc
     1141 actaccattg tgcatgttat gcgcctagga tggccaaaga cccattccgg ctgcatcgcg
     1201 caccgttttg ggctggctcg cttattgcta accttctggc actgaccggc ggttttccgc
     1261 tggttgcgac cgacccacat aaagcccttt cgccatgccc gataccgcct ttgccggcct
     1321 ggaactgctg gaaaccgcca cgctggccgt ctgccgcgac ggcggcatcc gctacgtcaa
     1381 tccggcctgc gaaaacctgc tggcgttgag ccagaaagaa ctgctccgcc acaccctgtc
     1441 cagcctgttt gtcgatagcc gcgccttggt ggccggcctg cgcgccgctg tcactcacga
     1501 tgtcagcttc accgagcacg acctctcact gaacaccatc aaccagattt cactgcaagt
     1561 catctgcacg ctcaccccga tcaatgtggc tggctgcgcc gccttgatcg aactgcgtcc
     1621 gattgaacag cagctgaaaa tcgccaatga agaacgcctg ctgatccagc aggccgccaa
     1681 ccgcgagctg atccgcaatc tggcgcacga aatcaaaaac ccgctgggcg gcattcgcgg
     1741 cgccgcgcag ttgctggcgc atgagctggc cgaccggcct gatctgaaag aatatcttga
     1801 cgtcatcacc catgaggcgg tgcggctgca acagctgctg gaccgcctgc tggcgcccaa
     1861 ccgcaagcac atcctgggcg aggtgaatat tcacgaagtg ctggagcggg tgcgcagcgt
     1921 ggcgctggcc gaatacccgc agggcatcgc catccgccgc gactacgaca ccagcctgcc
     1981 ggcgctgacc gccgacaagg agcaactgat tcaagtggtg ctcaatatcg tgcgcaacgc
     2041 catccaggcc atgcacggac gcggccagat cgtgctgcgc acccgggtgg cccgccaggt
     2101 gacgctggcg cgcaagcgct accagctggc catgctgttg caaatcatcg acaacggccc
     2161 aggcattccc gcagaactga aagaccatat tttttatccg ctggtcaccg gccgcgccga
     2221 aggcaccggc ctggggctga ccatcgcaca aacctatgtc caccagcatg gcggcaccgt
     2281 ggagtttgac tcacgccccg gccacacctg ctttactgtg ctgctgccct ttggagacaa
     2341 aaaagcatga cgccttctgt ctggatcatc gacgacgaca aatccatccg ctgggtgctg
     2401 gaaaaagccc tcacccgcgc cggcatcagc tacgccagct tcaccaacgc cgacgacgcg
     2461 ctgaatgcgc tgtacgatac cctgcccaag gtgattctca gcgacatccg catgccgggc
     2521 accgacgggc tgaagtttct ggccacggtc aaggccgagc atcctaccct gccggtgatt
     2581 gtgatgaccg cgcactccga cctggattcc gccgtggcgg cgtttcaggg cggcgcgttt
     2641 gaatacctgc ccaagccgtt tgacgtcgat caggcggtgc aattggtcga gcgcgcgctg
     2701 ggcgaggccg agcgcgacaa tgccggcccg gacgccatgg acgccacccc ggtgatgctg
     2761 ggccaggcgc cggccatgca ggacgtgttc cgcgccattg gccggctgtc gcaatcgtcg
     2821 gtcaccgtgc tgatcaccgg cgaatcgggc agcggcaaag agctggtggc gcacgcgctg
     2881 catcgccact ccaaccgcgc cgccaaggcg tttatcgcta tcaacaccgc cgccattccg
     2941 cgcgacctgc tggagtccga gctgtttggc cacgagcgcg gctcgttcac cggcgccacc
     3001 acccagcgcc gtggccgctt tgaagaagcc gagggcggca cgctgtttct ggatgaaatc
     3061 ggcgacatgc ccaccgagct gcaaacccgc ttgctgcggg tgttgtccga tgggtttttc
     3121 tatcgcgtcg gcggccatca gccgatcaag gccaatgtgc gggtgattgc cgccacccat
     3181 caaaacctgg aggaccgggt caagcaaggg ctgttccgtg aggatttgtt ccaccgcctg
     3241 aacgtcatcc gcctgcgcct gccgcccttg cgcgagcgtc aggaagacgt tcccttgctg
     3301 gcgcggcact ttttgttcaa gagcgcgcag caattggggg tggagcccaa gcgcctgtcc
     3361 gacggcgcca tggacgtgct caagacctat cccttccccg gcaatgttcg ccaactggag
     3421 aatatctgcc actggctgac ggtgatggcg ccggggcaaa ccatcgagcc gggcgatttg
     3481 ccgccagaaa tgaaagaaac cccgctcagc gcgccggtgg gcgtggtcgg cggcgattgg
     3541 gtgtcggcgc tggagcacct ggccgagcag cgcctcaagc gcggcgaagt gggcattatc
     3601 gacagtctca ctcaccagtt tgaagccgcg ctgatcaaga ccgcgctggc gcataccggc
     3661 ggacgacggg tggaagccgc ccatctgctg ggcctgggcc gcaacaccct cacccgcaag
     3721 attcaagagc tgggcatcga cggcggccgc gacgacgaag ccggcgtgtg accccggctg
     3781 cgcgccgggc agccattgac gggctcccgg cgcgcagacg cttgaacttt cctgcttacc
     3841 ccatcatcat gacggtttgc cgcacagtgc tgacgcggcg cggtgtggca aaccgccaac
     3901 tgcctctctt taatttcttg gcgccgttca ccacacttag cgcagtggcc ctggccaggt
     3961 gcacgacctc agaccgtacg ggtacgtgca accacgtcag gggattttgt gagcggcgct
     4021 tatcctccaa gcctgcttgt cgtgccgaca cgtgaggact catgtcgatc tggatcaatg
     4081 gccaaccggc cacgcaactg gacgccagcg accgtggcct gagctacggc gatgggctgt
     4141 ttcgcaccct gcgcctgctg gatggcgcgc cgctggcctg gcgctggcac tgggcgcggc
     4201 tgcaacacga ttgcgccgcc ctgcgcctgc cctgccccga ggcggaattg ctgctggccg
     4261 agctggcggc ggtgagcgcc gagtggccgg acgcggtggc caagctgacg ctcacccgtg
     4321 gcgtgggtcc gcgcggctat gcgccgccgg ccgcgccgca gcccacccgc attgtgcagg
     4381 ccgggcccag agtggcgccg ccagccgagc gctacagtca gggcgtggcg gtgtacgcct
     4441 gcgcgctgcg cctgggcctg caaccagcgc tggccggggt caagcacctg aatcgcctgg
     4501 aaaacgtgct ggcccgcgcc gaatggcaag gcgatgaata cgccgaaggc ttgctgtgcg
     4561 acagcgaggg ctgggtgatc gaaggcacca tgagcaatct gtggattcgc cgggggcggg
     4621 aattgctcac tccccagctg gaccgctgcg gcgtgagcgg cgccgcgcgc gcctgggtgt
     4681 tcgacaccgc gcccaagctg gggtatcaag tgcgcgaatg gcgcttgcgc ccggccgatg
     4741 tgctggcggc ggacgaagcc atgctgtgca atagcctgat cggcttttgg ccgattgccc
     4801 gctatcagca gcgccgctgg agcgtgttta ccgccagcga ggcgctgaac agcgcttggc
     4861 tggcgccaga atcagcgatt tcaggctgaa ctgtactgct ggagaagcgc accgactgcc
     4921 tctgcccggc tggcgcggct ggcgtacact ggcggctggt tttgttcaga aagacacccc
     4981 accatgcacg ccagcaactt tgttcttgac gactatctca gccgcatcgg ctaccacggc
     5041 ccgcgcgacg ccagcgccgc caccctggcg cagctgatgc gcgcccagtt gtttaccgtg
     5101 ccgtttgaaa acctggacgt gcaagccggc aaaatcgtgt cgatgacgcc ggaagacatc
     5161 gtgcaaaaaa tcgtctatca gccacgcggc ggctactgct acgaagtgaa cggcctgttc
     5221 gccatggcct tgcaggcgct gggctttagc tacacttttt tggccgcgcg gccgatgttc
     5281 tacccggtgc gccgcccgaa aacccatatg gtgctgtgcg tgcgcgccga tggccgcgac
     5341 tggctgtgtg acttgggttt tggcagctat ggcatccgcg cgccgctggc gctggacgcg
     5401 ctggatagtg atattgccca ggatcacgac cgcttccgcc tcagccgcga tgcgcaaggc
     5461 gtatacctgc tgcaagcctg ggtggacggc gaatgggcca atcaatatgg ctttgacctg
     5521 cacccgcaag agtggatcga ctttgccccg gccaactatc tgaactccac ccaccccgag
     5581 gcgatttttg tgcaaaaact gctggtggtg cgccacaccc cgcgcggccg ccacatcctg
     5641 ctgggcgagc gtctgaaaac catcgaagac ggcgtcagcc agctgcgcga gttgggcgcg
     5701 gccgaggtgg ctgtggtctt gcgcgaggtg tttgccctgg cggcctgatg tcgctgccgg
     5761 ctcaatgcgt gtcgctgtcg gcctcggcat ccagcccgcg caaaaacgcc agcttgtcgg
     5821 caatcttgct ttccagcccg cgcggcacgg gttgatacca gcccggctcg gccatgcctt
     5881 cgggcagata gcgctcgcct gcggcgtagc cgtgcggctc gtcgtgggca tagcggtagg
     5941 cgtggccgtg gcccagctct ttcatcagct gggtgggggc gttgcgcaga tgcaccggga
     6001 cttcccggct tttatcctgc ttcacaaagg cgcgcgccgc gttataggcg gtgtagccgg
     6061 cgttgctctt ggcggcgatg gccagataaa tcaccgcctg gcccagcgcc agctcgcctt
     6121 ccgggctgcc cagccgctcg taggtcaagg cggcgtcgtt ggcgatttgc atggcgcgcg
     6181 ggtcggccag gccgatgtct tcccaggcca tgcgcacgat gcgccgcgct aggtaacgcg
     6241 ggtcggcgcc gccgtcgagc atgcgggtca gccagtacag cgcggcgtcc gggtgcgagc
     6301 cgcgcaccga tttgtgcagc gcagaaatct ggtcgtaaaa atggtcgccg cccttgtcaa
     6361 agcgccggct gttgacagtc agcgcgctgt caacaaactg ggcgtcgatg gcctccacct
     6421 gcgccgcctg ggcggcggcg gcgcattgct ccagtagatt cagcagccgg cgcgcgtcgc
     6481 cgtcggcgtg gcccaccagc gccgcttgcg ctgcctcgtc aaagcgcagc tgcggcagcg
     6541 ccttgcgctg ggcgcgggta agcaacagcc tgagctcgtc atcgcccagg gatttcagca
     6601 catacacctg cgcgcgcgac aacagcgcgg cgttgacctc aaacgatggg ttctcggtgg
     6661 tggcgccgat aaaggtcacc agcccgcttt cggtgtaggg caacagcgcg tcttgctggc
     6721 ttttgttgaa gcggtggatt tcatcgacaa acaaaatggt gcgcaccccg cgcgccagat
     6781 ggcgctgggc ggtgtccatg gcggcgcgga tgtctttcac cccggaaaac accgccgaca
     6841 gggcgatgaa ttcacagtga aacgcgctgg cggtcagccg cgccagcgtg gtcttgccca
     6901 cccccggcgg cccccaaaaa atcattgaat ggggcttgcc ggactgaaac gccagctgca
     6961 agggcttgcc ggggccgagc aaatggcgct ggccaatcac ctcgtccaga ctgtgcgggc
     7021 gcagggcttc ggccagcggc gcggcgggcg tctgggcaaa tagctcagct ggcgaggacg
     7081 cagaaaacaa atcgctcatg gcggctcggg caaaaataga cacggcaaga ttctgaccgg
     7141 cgcacggcgg ggctgcaagc gtgggcttac ggtgtctcgt gctatggtat gacgttcata
     7201 ctaagagccg ctaacaaaac cctcctggcg ttgttgtgtg accttgtcgt actacttgta
     7261 ctgtctgcgg tcacacgcct agctgtgttt tccctgtagg ttgttcact
//
LOCUS       DAOPYX010000144         7254 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_12485_length_7254_cov_8.476038, whole genome shotgun sequence.
ACCESSION   DAOPYX010000144 DAOPYX010000000
VERSION     DAOPYX010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 7254)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7254)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7254
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12485_length_7254_cov_8.476038"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            304..1434
                     /locus_tag="PKN55_08435"
     CDS             304..1434
                     /locus_tag="PKN55_08435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HNX71310.1"
                     /translation="MTALLTHPDPDGLLEYSVVYTDRALNHMSQRFQGVMRDISRLLK
                     VVYHADATAIVPGSGTFGMEAVARQFATEQKVLVIRNGWFSFRWTQIFDMGRIPADTR
                     VLKARQVGDGPQAPFAPAPIDEVVAAIREYRPAVVFAPHVETASGMMLPDDYLQAVGA
                     AVREVGGLFVLDCIASGTVWVNMQANLVDVLISAPQKGWSASPCGALIGLSERALARL
                     NDTTSTSFACDLKKWRQIMEAFETGGHAYHATMPTDALTVLRDVMLETEQYGFDKVCA
                     EQHELGRRVRALLESRGFPSVAAEGFQAPGVVVSYTSDPEIQTGKKFLGVGLQTAAGV
                     PLQCDEGADFRTFRIGLFGLDKLHDPARTVESLAEGLDKVLG"
     gene            1599..4280
                     /gene="gyrA"
                     /locus_tag="PKN55_08440"
     CDS             1599..4280
                     /gene="gyrA"
                     /locus_tag="PKN55_08440"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003824882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HNX71311.1"
                     /translation="MTEHLFAKETLPVSLEEEMRRSYLDYAMSVIVGRALPDVRDGLK
                     PVHRRVLFAMHELSNDWNRAYKKSARIVGDVIGKYHPHGDMAVYDTIVRMAQDFSLRY
                     PLVDGQGNFGSVDGDNAAAMRYTEIRMARIAHELLADIEKETVDFGPNYDGSEHEPLI
                     LPAKIPNLLINGSSGIAVGMATNIPPHNLNEVVDACLALLGNSEMTIDELIDIIPAPD
                     FPTAGIIYGTAGVKEGYRTGRGRVVMRARTHVEDIGKGDRQALIVDEIPYQVNKARLL
                     ERIGELVREKTLEGISEIRDESDKSGMRVVIELKRGEVPDVVLNNLFKMTQLQDSFGI
                     NMVALVDGQPRLLNIKQILEEFLRHRREVVTRRTVYELRKARERGHVLEGLAVALSNV
                     DPIITLIKASPTPAEAKRELMGQNWRSELVAGMLAKVDPSQARPDGLLPEYGLHDDAY
                     RLSDVQAQAILELRLQRLTGLEQDKIVDEYRDVMTTIIDLSDILARTERVSDIIAAEL
                     NQVKTQFGDARRSEISVFGGDINIEDLITPQDMVVTISHGGYIKAQPLDDYRAQRRGG
                     RGKQAAATKDDDFIETLFAANTHDYLLCFSSLGRMYWIKVYDLPQGSRNSRGKPMVNV
                     LPLADGEKINALLPVKAFTDAEADVKDDIEDEEAAVKAKGPFIFMCTASGTVKKTPLQ
                     AFSRPRAAGIIAVKLDEGDNLIGVALTSGHDQVMLFSNAGKAVRFDETAVRPMGRTAA
                     GVRGMQLGEDQQVISLLVTSSEDQQVLTASDGGYGKRTRVADFRQTSRGTQGVIAMDL
                     TDKTGLKLVAASLVEETDDIMLITDGGVLIRTKAAEVRETGRAAQGVRLINLDDGERL
                     VSIVKVAESDDADADAELDADAASPTEGGDTSTDTPA"
     gene            4277..4735
                     /locus_tag="PKN55_08445"
     CDS             4277..4735
                     /locus_tag="PKN55_08445"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012697307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HNX71312.1"
                     /translation="MTELLDTLQRWFVSLPHCATLGFEFIGVEDGKPTLKVNYRDDLV
                     GNGSNGVIHGGVITSLVDTTSALSVFSHLDSAEAMATLDLRIDYLKAATPGLPIYCTA
                     ECYKLARQIGFTRAVCHHGDPADPIAHGVATFMRGSSNAILGGDATGAPL"
     gene            4744..5169
                     /locus_tag="PKN55_08450"
     CDS             4744..5169
                     /locus_tag="PKN55_08450"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014087315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HNX71313.1"
                     /translation="MQSFLALRERKDYAGIIDSIPYARLMGVEFEESGEDSLLFRLPY
                     YERNIGNTLLPALHGGLIGGFLENAGLIHLMWVRATLEPPKVVDISFDYLRSGRPQTL
                     YAQCQLTKQGKRVANMLIECWQNDRAKPVAVARAHFLLS"
     gene            5213..6295
                     /gene="serC"
                     /locus_tag="PKN55_08455"
     CDS             5213..6295
                     /gene="serC"
                     /locus_tag="PKN55_08455"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011135848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HNX71314.1"
                     /translation="MTQVYNFSAGPAVLPRDVLLQAQQELLDWHGSGMSVMEMSHRGK
                     EFMSIIHEAETDLRQLMGIPANYKVLFLQGGALSQFAMVPLNLLGDKKSIDVVNTGHW
                     SKLAIKEARKFAEVNIVASSEDRQYTYVPDPASWRTSPDAAYLHYVSNETIGGVQFPY
                     IPDSTVPLVCDMSSDILSRAVDVSRFGLIFAGAQKNIGPSGLTLVIVREDLLGQLPAH
                     MPTMYDYRVHADADSMYNTPPTYAIYIAGLVFKWLKQQGGVPGMASRNEEKAGLLYHA
                     IDSSQGFYHCPVETPFRSRMNVPFRLGSPELEAAFLAEAEQRNLLQLKGHRSVGGMRA
                     SIYNAMPLEGVKTLASFMVDFARRHG"
     gene            6304..6891
                     /locus_tag="PKN55_08460"
     CDS             6304..6891
                     /locus_tag="PKN55_08460"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014087317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNX71315.1"
                     /translation="MLTPALLQQLDRLAQHGQKHDAKTLTRADKMLNITPDTGMMLYQ
                     WLRFGRPAHVVEIGTSNGYSTLWLAAAAQAYGGRVTSLDVLQAKTTLARNNLNEAGLL
                     DTVTLVTAEAGGWLRQQPAESAQLIFLDADRSLYASYWPLLTPVLAPGGLIVVDNAVS
                     HAAECADFFARVRATPGFLCELLPIGKGEFIILKD"
     gene            6895..>7254
                     /gene="pheA"
                     /locus_tag="PKN55_08465"
     CDS             6895..>7254
                     /gene="pheA"
                     /locus_tag="PKN55_08465"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008952724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HNX71316.1"
                     /translation="MSDDLLKQHRDAIDSIDAQILELINQRARHAQKIGEIKGGGVIY
                     RPEREAQVLRRIKDINPGPLPDESAARLFREIMSACLSLEKPLSIAYLGPEGTFTQAA
                     AIKHFGHAANTVACASID"
BASE COUNT         1414 a         2389 c         2229 g         1222 t
ORIGIN      
        1 tgctggagca actcagccgc gaagccggcg tgcaagtggg cggcaagctc tatgctgatg
       61 ccttgtcccc ggccaatggc ccggcgccca gttatctcaa gctgttcagc cataacgtca
      121 acagcctgag cgcggcgatg aagccgtaag cgtctcctgg cgtggctgcc cggcctcagg
      181 gccgctgcgc tgggcttggc gcgccgcgcg cgacacttgc cacaaacccg tccgctggcg
      241 gtatgcttat gcccttggtt tgcgccgccg cgcggcgcag ccagactacc aaggagaaac
      301 caagtgactg ccctgctgac ccatcccgat cccgacggcc tgctcgaata ttccgtggtc
      361 tacaccgacc gcgcgctcaa ccacatgtcg cagcgctttc aaggggtgat gcgcgacatc
      421 tcgcgcctgc tcaaagtggt gtaccacgcc gacgccaccg ccatcgtgcc cggcagcggc
      481 acctttggca tggaagccgt cgcccgccag ttcgccaccg agcaaaaagt gctggtgatc
      541 cgcaatggct ggttcagctt ccgctggacg cagatttttg acatgggccg catcccggcc
      601 gacacccgcg tgctcaaggc ccgccaagtg ggcgacggcc cgcaagcgcc atttgccccg
      661 gccccgattg acgaagtggt cgccgccatc cgcgaatacc gcccggccgt ggtgtttgcc
      721 ccgcacgtgg aaaccgcctc cggcatgatg ctgccggatg attacctgca agccgtcggc
      781 gccgccgtgc gcgaagtggg cggcctgttt gtgctggact gtatcgcctc tggcacggtg
      841 tgggtgaata tgcaggccaa tctggtggac gtgctgatca gcgccccgca aaaaggctgg
      901 agcgcctcgc cctgcggcgc gctgattggc ctgagcgaac gcgcgctggc gcggctgaac
      961 gacaccacca gcaccagctt tgcctgcgac ctgaaaaaat ggcggcaaat catggaagcc
     1021 tttgaaaccg gcggccacgc ctaccacgcc accatgccca ccgacgcgct gaccgtgctg
     1081 cgcgatgtga tgctggaaac cgagcaatac ggctttgaca aagtctgcgc cgaacagcac
     1141 gaactcggcc gccgcgtgcg cgccctgctg gaatcgcgcg gcttccccag cgtcgccgcc
     1201 gaaggcttcc aggcccctgg cgtggtggtc agctacacca gcgacccgga gattcaaacc
     1261 ggcaagaaat tcctcggcgt cggcctgcaa accgccgccg gcgtgccgct gcaatgcgac
     1321 gaaggcgccg acttccgcac cttccgcatc ggcctgttcg ggctggacaa actgcacgac
     1381 ccggcgcgca cggtggagag cctggcggag ggattggata aggtgttggg ctaaatccgg
     1441 ctgtatttct gaactgaact cgcccgcgcc gccctctcct gcggcgcggc aatccctgcc
     1501 tgctggctgc tttcgcgccc gtcccatccc ctgtcatcgc cctgacatgc tagaatcagc
     1561 ccttactttt gcttagaacc agacggcttg cccgatccat gacagaacat ctattcgcca
     1621 aagaaaccct tccggtcagc ctcgaagagg agatgcggcg ttcctacctg gattacgcga
     1681 tgagcgtgat cgtgggccgg gcgctgcctg atgtgcgcga cggcctcaag cctgtgcatc
     1741 gccgcgtgct gtttgccatg cacgagctgt ccaacgactg gaaccgggct tacaagaagt
     1801 cggcgcgtat cgtcggcgac gtgatcggta aataccaccc gcacggcgac atggcggtgt
     1861 acgacaccat cgtgcgcatg gcgcaggatt tttcgctgcg ctaccccctg gtggacggcc
     1921 agggcaactt cggctcggtg gatggcgaca acgccgccgc catgcgttac accgaaatcc
     1981 gcatggcgcg catcgcccac gagctgctgg ccgacatcga aaaagaaacc gtcgattttg
     2041 gccccaacta cgacggctcg gagcacgagc cgctgatcct gccggccaaa atccccaatc
     2101 tgctgatcaa cggcagctcg ggcattgccg tgggcatggc caccaatatt ccgccgcaca
     2161 acctcaacga ggtggtggac gcctgcctgg cgctgctggg caatagcgag atgacgattg
     2221 acgagctgat cgacatcatc ccggcgccgg actttcccac cgccggcatc atctacggca
     2281 ccgccggggt gaaagaaggc taccgcaccg gccgtggccg ggtggtgatg cgcgcgcgca
     2341 cccatgtcga agacatcggc aagggcgacc gccaggcgct gatcgtcgat gaaattccct
     2401 accaagtgaa caaggcgcgc ctgctggagc gcattggcga gctggtgcgg gaaaaaaccc
     2461 tggaaggcat cagcgaaatc cgcgacgagt cggacaaatc cggcatgcgc gtggtgattg
     2521 agctcaagcg cggcgaagtg ccggacgtgg tgctgaacaa tctgttcaag atgacccagc
     2581 tgcaagacag ctttggcatc aatatggtgg cgctggtcga tggccagccg cgtctgctga
     2641 atatcaagca gattctggaa gaattcctgc gccatcgccg cgaagtggtc acccgccgca
     2701 cggtgtacga gctgcgcaag gcgcgcgagc gcggccatgt gctggaaggc ctggcggtgg
     2761 ccttgtccaa tgtggacccg atcatcaccc tgatcaaggc ctcgcccacc ccggccgagg
     2821 ccaagcgcga gctgatggga caaaactggc gctccgagct ggtggccggc atgctggcca
     2881 aggtggatcc aagccaggcg cgcccggatg ggctgctgcc ggaatacggc ctgcacgacg
     2941 acgcgtatcg tctgtccgat gtgcaggcgc aggcgattct ggaattgcgc ctgcaacggc
     3001 tgaccgggct ggagcaggac aaaatcgtcg atgaataccg cgatgtgatg accaccatca
     3061 tcgacctgag cgacatcctg gcccgcactg agcgggtgtc ggacatcatc gccgccgagc
     3121 tgaaccaggt aaaaacccag tttggcgacg cgcgccgttc ggaaatctcg gtatttggcg
     3181 gcgacatcaa tatcgaagac ctgatcaccc cgcaagacat ggtggtgacc atctcgcacg
     3241 gcggctatat caaggcgcag ccgctggacg actaccgcgc ccagcgccgt ggcggacgcg
     3301 gcaagcaggc ggcggccacc aaggacgacg atttcatcga aaccctgttt gccgccaaca
     3361 cccacgacta tctgctgtgc ttctccagcc tgggccgcat gtactggatc aaggtctacg
     3421 acctgccgca gggcagccgc aacagccgtg gcaagccgat ggtcaatgtg ctgccgctgg
     3481 ccgatggcga aaaaatcaac gccctgctgc cggtcaaggc gtttaccgac gccgaggcgg
     3541 acgtcaagga cgacatcgag gacgaagaag ccgcggtcaa ggccaagggc ccgttcatct
     3601 tcatgtgcac cgccagcggc acggtgaaaa aaaccccgct gcaagcgttc tcccgcccgc
     3661 gcgcggccgg catcatcgcc gtcaagctgg acgaaggcga caatctgatt ggcgtggcgc
     3721 tgacctcggg ccacgaccag gtgatgctgt tctccaacgc cggcaaggcc gtgcgctttg
     3781 acgaaaccgc cgtgcgcccg atgggccgca ccgccgctgg cgtgcgcggc atgcagctgg
     3841 gcgaagacca gcaggtgatt tccctgctgg tgaccagctc ggaagaccag caagtgctca
     3901 ccgccagcga tggcggctat ggcaagcgca cccgcgtggc cgatttccgc cagaccagcc
     3961 gcggcacgca aggcgtgatc gccatggacc tcaccgacaa aaccggcctg aagctggtgg
     4021 cggccagcct ggtggaagaa accgacgaca tcatgctgat caccgacggc ggcgtgctga
     4081 tccgcaccaa ggcagcagaa gtgcgcgaaa ccggccgcgc cgcccagggc gtgcgcctga
     4141 tcaatctgga cgacggcgag cgattggtca gcatcgtcaa ggtggccgag agcgacgacg
     4201 ccgacgccga cgccgaactc gacgccgacg ccgccagccc gacggaaggc ggcgacacca
     4261 gcacggacac cccggcatga ccgaactgct cgacaccctg caacgctggt ttgtcagctt
     4321 gccgcactgc gccaccttgg gctttgagtt cattggcgtg gaagacggca agccgacgct
     4381 caaggtgaac taccgtgatg atctggtggg caacggcagc aatggcgtga ttcacggcgg
     4441 ggtgatcacc tcgctggtgg acaccaccag cgcgctgtcg gtgttcagcc acctggacag
     4501 cgccgaggcg atggccacgc tggatttgcg catcgactac ctgaaggccg ctacgccggg
     4561 cctgccgatt tactgcacgg cggagtgcta caagctggcg cggcaaatcg gctttacccg
     4621 cgcggtgtgc catcacggcg accccgccga cccgattgcc cacggcgtgg ccacctttat
     4681 gcgcggctcg tccaacgcca tcctgggcgg cgacgccacc ggagccccgt tatgagcgcc
     4741 ttcatgcaat ccttcctggc gctgcgtgag cgcaaggact atgctggcat catcgacagc
     4801 atcccctacg cccggctgat gggcgtggaa tttgaagaaa gcggcgaaga cagcctgctg
     4861 tttcgcctgc cgtactacga gcgcaatatc ggcaacaccc tgctgccggc gctgcacggc
     4921 gggctgattg gcggttttct ggaaaacgcc ggactgatcc acctgatgtg ggtgcgcgcc
     4981 acactggagc cacccaaggt ggtggatatc tcgtttgact acctgcgctc gggccggccg
     5041 caaacgctat acgcgcaatg ccagctgacc aagcagggca agcgggtggc gaacatgctg
     5101 attgagtgct ggcagaatga ccgcgccaag ccggtggcag tggcgcgcgc ccactttctg
     5161 ctgagctgaa acaccccggc acatccggga tagacgaaac aaggacggct tgatgaccca
     5221 agtgtacaac ttctccgcag ggccggcggt gctgccgcgc gatgtgttgc tgcaagccca
     5281 gcaagagctg ctggattggc acggcagcgg catgtcggtg atggaaatga gccatcgcgg
     5341 caaagaattc atgtccatca tccacgaagc agaaaccgac ctgcgccagc tgatgggcat
     5401 tccggccaat tataaagtgc tgtttttgca gggcggcgcg ctgtcgcagt ttgccatggt
     5461 gccgctgaac ctgctgggcg acaaaaaatc cattgatgtg gtcaacaccg gccactggtc
     5521 caagctggcc atcaaagaag cgcgcaaatt cgccgaggtg aatatcgtcg cctccagcga
     5581 agaccgccag tacacctatg tgccggaccc ggccagctgg cgcacctcgc cggacgcggc
     5641 ctatctgcac tatgtgtcca acgaaaccat cggcggcgtg cagttcccgt atatcccgga
     5701 cagcactgta ccgctggtgt gcgatatgtc gtcggatatt ctgtcgcgcg cggtggacgt
     5761 gtcgcgcttt gggctgattt ttgccggcgc ccaaaagaac atcggcccgt ccggcctcac
     5821 cctggtgatt gtgcgcgaag acctgctggg ccagctgccg gcgcatatgc ccaccatgta
     5881 cgactaccgc gtgcacgccg acgccgactc gatgtacaac accccgccga cctacgcgat
     5941 ttatatcgcc ggcctggtgt tcaagtggct caagcagcaa ggcggcgtgc cgggcatggc
     6001 cagccgcaac gaagaaaaag ccggcctgct gtatcacgcc atcgacagca gccagggttt
     6061 ttaccactgc ccggtggaaa ccccgttccg ctcgcgcatg aatgtgccgt tccgcctggg
     6121 ctcgcccgag ctggaagccg ccttcctggc cgaagccgag cagcgcaatc tgttgcagct
     6181 caagggccat cgttcggtcg gcggcatgcg cgcgtcgatt tacaacgcca tgccgctgga
     6241 aggggtgaaa accctggcca gcttcatggt ggactttgcc cgccgccacg gctgaggccc
     6301 gccatgctga cgccagcctt gctccagcag ctggaccgac tggcccagca cggccagaaa
     6361 cacgacgcca aaacgctaac ccgcgccgac aagatgctca acatcacccc ggataccggg
     6421 atgatgctgt atcagtggct gcgttttggc cgcccggcgc atgtggtgga aattggcacg
     6481 tccaatggct attccaccct gtggctggcc gccgccgccc aggcgtatgg cggccgcgtc
     6541 accagcctgg acgtactgca ggccaagacc accctggcac gcaacaacct caacgaagcc
     6601 ggcctgctcg acaccgtgac cttggtcact gccgaggcgg gcggttggct gcgccagcag
     6661 ccggcggaat ccgcccagct gatttttctg gacgccgacc gcagcctgta cgccagctat
     6721 tggccgctgc tgaccccggt gctggcgccg ggcggcttga ttgtggtgga caacgccgtg
     6781 tctcatgcgg ctgaatgtgc agactttttc gcccgggtgc gcgccacgcc gggctttttg
     6841 tgcgaactgc ttcccatcgg gaagggtgaa ttcatcattc tcaaagactg acccatgtcc
     6901 gacgacctgc tcaaacaaca ccgcgacgcc atcgacagca tcgacgccca gattctggaa
     6961 ctgatcaacc agcgcgcccg ccatgcccag aagattggcg aaatcaaggg cggcggggtg
     7021 atctaccgtc ccgagcgcga agcccaggtg ctgcgccgga tcaaggacat caatcccggc
     7081 ccgctgcccg acgaatccgc cgcgcgcttg ttccgcgaaa tcatgtcggc ctgtctgtcg
     7141 ctggaaaaac cactgtccat tgcctacctc ggccccgaag gcacgtttac ccaagccgcc
     7201 gccatcaagc actttggcca tgccgccaac accgtggcct gcgcctcgat tgac
//
LOCUS       DAOPYX010000145         7210 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_12596_length_7210_cov_12.240391, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000145 DAOPYX010000000
VERSION     DAOPYX010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 7210)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7210)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7210
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12596_length_7210_cov_12.240391"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1197
                     /locus_tag="PKN55_08470"
     CDS             <1..1197
                     /locus_tag="PKN55_08470"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNX71317.1"
                     /translation="IMATHSPPTPAKSWLPMSLAGRAWLAFALLLLATVPLVLAVEPA
                     KQLSAWSLAALGWLAALIAGMVMVRIAHQDWQSLRNALTALAERNLSQRVHLYRTDTV
                     GAVGDWLNLSTRRLADIFVDLQRAGAELRHAAGEVSHAAGRLSHSMDQQRDLTLSSTA
                     TLEQLSASLDNMSVQMQDTAASASASHQAASEGEREVSEVAAAMADLAAETRALRLTL
                     DGLHQRSGEITQVVELIREVAAQTNLLALNASIEAARAGESGRGFAVVADEVRNLAQR
                     TQVATEDITQVVAAIGADVGHAVARIEICQDKAEHSSAQAGAAAQRLAGICAVTADTH
                     ALAGQMTLAIDEQRDASHALTRNVEARDVLAQENARHVHETADVSRYLEQLAIKLEKL
                     VGAHTL"
     gene            1227..2114
                     /locus_tag="PKN55_08475"
     CDS             1227..2114
                     /locus_tag="PKN55_08475"
                     /inference="COORDINATES: protein motif:HMM:NF019974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate- and nitrite sensing domain-containing
                     protein"
                     /protein_id="HNX71318.1"
                     /translation="MEALELLPIASMTLLGAATAVRCRLFGQRWRQRRQQDGVQWCGA
                     LQNLVMHLQRHRGLSSMWLNGDRSVGPRLHAEREQANQHMHTLAQLPDGHLSAADVLP
                     RAQWERFCADWRALCAELEQLDAADSIARHTELISQALNWLRAIGEASLSAGPQDHGW
                     VSALVDQLPALSEALGQARAISAGVAARGQCTAVARVRLAYLASRIETLNGACRQALA
                     ASQHSRLPGMRDGLARIDAGVQRTLQCLASQLTGCAGANGADCFGVCTEAIDAVFALM
                     SGLLAGARSAQPGALPLAA"
     gene            2851..3219
                     /locus_tag="PKN55_08480"
     CDS             2851..3219
                     /locus_tag="PKN55_08480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002925461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNX71319.1"
                     /translation="MSKRIFLVDDSATILLSISNILTKAGFEIEKAANAEEALRKFQS
                     GVKVDLLITDLNMPGMNGIELIKEVRKLPNYRFMPILFLTTESQQTKRAEAKAAGASG
                     WLVKPATAESLLNTIKLVLR"
     gene            3231..4520
                     /locus_tag="PKN55_08485"
     CDS             3231..4520
                     /locus_tag="PKN55_08485"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNX71320.1"
                     /translation="MSPSSPRKPRQIMLRSIMVTLGVSVVTCWFVFLAHGWFHATLLP
                     ILGISVDLGHAIGTMLAMLAALVGQLVVSRTLFNDEMFGVAHMQTRMREEREKIRLVA
                     TEVADELSSVKTFDDVVRGQLDNVVAQTEAAAFDMTQRLQTIDQVVSRLDGFVTRTTS
                     ESLQRIEDSEASIAQNRAMIGKMTEYIQRRIAAAETEQQRVAQVVLEARALEALVQLI
                     RDIAAQTNLLALNAAIEAARAGEAGRGFAVVADEVRKLSQESDNAVTRINQGIQEVAS
                     TIEEQFSEALSTQQVEKERAILKVFSEQMAELSRSYEEATDNVKTVLATVRECSTELS
                     SMFMDTLASVQFQDVTRQQLEHVGAALRRLDSHAELLAERLRAFDNPHADVTPLAEHL
                     SAMFDQYVMSQQRDSHRRSLKDGSTSQDSDGPKVELF"
     gene            complement(4613..5104)
                     /locus_tag="PKN55_08490"
     CDS             complement(4613..5104)
                     /locus_tag="PKN55_08490"
                     /inference="COORDINATES: protein motif:HMM:NF024252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNX71321.1"
                     /translation="MAIVFHIAESYSYKSSDICTLSLMIGHRIREARKQRGYNQQWLA
                     EQIGVSQPSVSDWESGRNDPTMDNLAMAARVLDVHIEWLATGRGPRDYADMLGELACQ
                     EKTGEYNYSRKLQPPVPPSRQETQEFSRLLRRLQPEQRVLLLGVLRSLVDSEKTDGEK
                     PAK"
     gene            5668..5982
                     /locus_tag="PKN55_08495"
     CDS             5668..5982
                     /locus_tag="PKN55_08495"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNX71322.1"
                     /translation="MTMLTPTLYADNDLTIYHAQAIKQRWLEGLHQSEGDVTLDLSRV
                     GEIDTAGLQLLWLLRREAHASGRQLNIVAVSPAVADLLAFCRLTENFATPRATEPEVE
                     AA"
     gene            5988..>7210
                     /locus_tag="PKN55_08500"
     CDS             5988..>7210
                     /locus_tag="PKN55_08500"
                     /inference="COORDINATES: protein motif:HMM:NF013767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HNX71323.1"
                     /translation="MDELTGVFVQESREQLAEMEAGLLALEANPEETGDINAIFRAAH
                     TIKGGAGVVECSYLESFTHKVENLLDQLRDGRLSPSEMRISLLLDCADHIGLLLDALE
                     QGETALSTSVIDADSRLRERLELESTPPGARVTAPPAEAPAPAPGNEATADIWHISVR
                     FGEDVLRHGMDPIAFLRFLTTLGEIRELQTLADTMPPAAQMDPEACYLGFEIRLETSA
                     GKPEIEAVFDFVRDECELHILPPHSRSEDYIALINALPDDERPLLGEMLLRAGVLTES
                     ELLIGLNSQELAKAYTDNSPPIGAILVEHKSVRPEVVEAAIQRQSAIQQAQQQTAPPP
                     RVIEHEEVSAHIEPVTVPAVVADGEHHPAPAKARAASSEARMLRVQAEKLDRLIDLVG
                     ELVIAGASATELARQ"
BASE COUNT         1477 a         2317 c         2135 g         1281 t
ORIGIN      
        1 atcatggcta cacattcacc ccccacgcca gccaagagct ggctgcccat gtcgctggcc
       61 ggccgcgcct ggctggcttt tgccctgctg ttgctggcca ccgtccccct ggtgctggcg
      121 gtcgagccgg ccaaacaatt gtctgcctgg tcgctggcgg cgctgggctg gctggccgcg
      181 ctgatcgccg gcatggtcat ggtgcgcatc gctcaccaag actggcaatc gctgcgcaat
      241 gcgctgaccg cgctggccga gcgcaacctc agccagcgtg tgcacctgta ccgcaccgac
      301 acggtgggcg cggtgggcga ctggctcaac ctcagcaccc gccgtctggc cgatatcttt
      361 gtggatttgc agcgcgctgg cgccgagttg cgccacgccg caggcgaagt cagccacgcc
      421 gccggccggc tcagccacag tatggatcag cagcgcgacc tgaccctgtc gtccaccgcc
      481 accctcgaac agctcagcgc cagcctggac aatatgtcgg tgcaaatgca ggacaccgcc
      541 gccagcgcca gcgccagcca tcaggccgcc agcgaaggcg agcgcgaggt cagcgaggtg
      601 gcggcggcca tggccgatct ggcggcggaa acccgcgcct tgcggctgac cctggacggc
      661 ctgcatcagc gctcgggcga aatcacccag gtggtggagc tgattcgcga ggtggccgcg
      721 caaaccaatc tgctggcgct gaacgcctcg attgaggccg cccgcgccgg cgaatccggc
      781 cggggctttg ccgtggtggc cgacgaagtg cgcaacctgg cccagcgcac gcaagtggcc
      841 accgaagaca tcacccaggt ggtggccgcc attggcgccg acgtgggcca cgccgtggcg
      901 cgcattgaaa tctgccagga caaggccgag cacagcagcg cccaggccgg cgccgccgcc
      961 cagcgcctgg ccggcatctg cgcagtcacc gccgacaccc acgcgctggc cggacaaatg
     1021 acgctggcga ttgacgagca acgcgacgcc agccatgcgc tcacccgcaa tgtcgaagcg
     1081 cgcgacgtgc tcgcccagga aaacgcccgc cacgtgcacg aaaccgccga tgtgtcgcgc
     1141 tacctcgaac aactggccat caaactggaa aagctggtcg gcgcgcacac gctgtgacgc
     1201 ttgccccatt gccaaggaga ttccccatgg aagccctcga actcttgccg attgccagca
     1261 tgaccctgct gggcgccgcc accgccgtgc gttgccgttt gtttggccag cgctggcgcc
     1321 agcgccgtca acaggatggc gtgcaatggt gcggcgcctt gcagaacctg gtgatgcatc
     1381 tgcaacgcca tcgcggtttg tccagcatgt ggctgaacgg cgaccgctcg gtggggccgc
     1441 gtctgcacgc cgagcgcgag caggccaacc agcacatgca caccctggcg caactgccgg
     1501 atggccacct cagcgcggcg gatgtgttgc cgcgcgcgca gtgggagcgc ttttgcgccg
     1561 actggcgcgc cttgtgcgcc gagctggagc aactggacgc cgccgacagc atcgcccgcc
     1621 acaccgagct gatcagccag gcgctgaact ggctgcgcgc catcggcgag gccagcctga
     1681 gcgccggccc gcaggatcat ggctgggtca gcgccctggt ggatcagctg ccggctttgt
     1741 ccgaagcgct gggccaggcg cgcgccatca gcgccggcgt ggccgcgcgc ggccagtgca
     1801 ctgccgtggc gcgggtgcgg ctggcctatc tggccagccg catcgaaacc ctgaacggcg
     1861 cctgccgtca ggcgctggcc gccagccagc acagccgcct gcccggcatg cgcgatggcc
     1921 tggcgcgcat tgacgccggc gtgcagcgca ccctgcaatg cctggccagc caactgaccg
     1981 gctgcgccgg ggccaatggc gcggattgct ttggcgtatg caccgaagcc atcgacgccg
     2041 tgtttgcctt gatgtccggt ttgttggccg gcgcccgttc ggcacagcct ggcgccttgc
     2101 cgctggccgc ctgagcacgg cagaccaaac cacttgcaac cgccctgtgt gccgtcagcg
     2161 ccatctggtc gcgcccagga tggccgggcc agacgctgca tgcagcccgg cgcgggcgcg
     2221 gtaagcccct catgtggttt ttagtgcaaa aatgctcgca tggcataggc acgctgacgt
     2281 ccgctcaacg ccgctgtgcc aacctggcag gcaggtatct ctgatttctt cacaaaacga
     2341 caagtgtttg tttacactgc aatgacagcc cagaaaacag cagtctctgc cgcagatatt
     2401 ccagacatgg ccacaccatg gcggaatggc cgggcgcggc cgacataaat tgcgactgga
     2461 cactggccac agattcagta attctacggt tgccaagctg gccaatcagt gatgaagtct
     2521 tgcactggct ggaaaaacct tgaccggcat caacagcacc cccatgtggg ctcgtagaat
     2581 gcacggcttg ttttctgact gtttgatgtt tccgccacat tgtcgtgtca atccaggcag
     2641 ccgtcagggt aacggcctgg gtctggcagc gctccccgtg caacgcttcg cccctgcgcg
     2701 cccttttggt gtgccaacgc agggcggatg tcgtaccggt cagatgccgc cagcacacaa
     2761 gacatcgtgc atgtcttgga gacgacctgt tgaatcgcgg cgcaagccgc cggatggctt
     2821 gcgacgacct gaagaaagag ttacccgacc atgagtaaac gcatttttct ggtggatgat
     2881 tccgccacga tcctgctgag tatttccaat atcctgacca aggccgggtt tgaaatcgaa
     2941 aaagccgcca acgccgaaga agcgttgcgc aagtttcaat ccggcgtgaa ggtggatctg
     3001 ctgatcaccg acctgaacat gccgggcatg aatggcatcg agttgatcaa ggaagtgcgc
     3061 aagctgccca attatcgctt catgccgatt ctgtttctga ctaccgaatc gcaacagacc
     3121 aagcgcgccg aggccaaggc cgccggcgcc tccggctggc tggtgaaacc cgccacagcc
     3181 gaatcgctgc tgaacaccat caaactcgtg ctgcgctgag gcttttgacc atgtcgccat
     3241 cttctccacg caaaccccgg cagatcatgc tgcgctccat catggtgacg ctgggcgtct
     3301 ccgtggtgac ctgctggttc gtgtttcttg cccatggctg gttccacgcc accctgctgc
     3361 cgatactggg catcagcgtg gacctgggac acgccattgg caccatgctg gccatgctgg
     3421 cggcactggt cggccaactg gtggtgtcac gcacgctgtt caacgacgag atgttcggcg
     3481 tggcccatat gcagacccgg atgcgtgaag aacgcgagaa aatccgcctg gtggccaccg
     3541 aagtggcgga tgaattatct tcggtaaaaa ccttcgatga tgtggtgcgc ggccagttgg
     3601 acaatgtggt ggcacaaacc gaagccgccg cctttgacat gacccagcgc ctgcaaacca
     3661 ttgatcaggt ggtcagccgg ctggatggct ttgttacccg caccaccagc gaatcgctgc
     3721 aacgcatcga agattcggaa gccagcattg cccaaaaccg ggcgatgatc ggcaagatga
     3781 ccgaatacat ccagcgccgc attgccgcag cagaaaccga gcaacaacgt gtggcgcaag
     3841 tggtgttgga agcccgggcg ctggaagccc tggtgcagct gatccgcgac atcgccgccc
     3901 aaaccaatct gctggccctg aatgctgcca ttgaggccgc ccgcgcgggc gaggccgggc
     3961 gtggttttgc cgtggtggcc gatgaggtgc gcaaactttc gcaagaaagt gacaacgccg
     4021 tcacccgtat caatcagggt attcaggaag tggcgtccac catcgaagag caattcagcg
     4081 aagccctgtc cacccagcag gtggaaaaag aacgcgccat cctcaaggtg ttttccgaac
     4141 agatggccga actgtcgcgc agctacgaag aagccaccga caacgtcaag accgtgctgg
     4201 ccaccgtgcg cgaatgcagc acggagctgt cgtcgatgtt catggatacc ctggccagcg
     4261 tgcagtttca ggatgtcacc cgccaacagc tggaacatgt cggcgcggcc ttgcgtcggc
     4321 tggacagcca tgccgaactg ctggccgagc gcctgcgcgc ctttgacaac ccgcatgccg
     4381 acgttacccc gctggccgag cacctgagcg ccatgttcga ccagtacgtg atgtcccaac
     4441 agcgggacag ccaccgacgc tcgctgaagg atggctcgac cagccaggac agcgacggcc
     4501 cgaaagtgga actgttctga ttctgttctc cccaaaacat cagggctcgc cgaataccat
     4561 cggcgagccc tgatgtttta tggccactgg ctccggccac aggagggaga gatcatttag
     4621 cgggtttttc cccgtcggtt ttttcgctgt ccaccaggct gcgcaatacg cccagcaata
     4681 acacccgctg ttctggctgc aaacggcgca gcaagcggga aaactcttgt gtttcctggc
     4741 gggaaggcgg caccggcggc tgcaattttc gcgaataatt atattcgcct gtcttttcct
     4801 ggcaggccag ctcaccaagc atgtcagcat aatctcgcgg gccgcgacct gtagccagcc
     4861 attcaatatg aacgtcaagc acacgcgccg ccatggcaag attatccatg gtgggatcat
     4921 tgcggccgga ttcccagtcg ctgacggagg gttgagaaac gccaatttgc tcggccagcc
     4981 attgttggtt gtaaccgcgc tgttttctgg cttctcgaat gcgatgtcca atcatgagcg
     5041 acagtgtaca aatgtcgctt gatttatagg aatagctttc agctatatga aaaacaatag
     5101 ccaaacatca ttaacattca atgcgattga aatatagttg attgatatat ttaattccgc
     5161 accacatagc acccaccaag tgcaccatgt ccattcatta tactggtata ccaatgataa
     5221 aaaaccatat ttatgacaat aaccggaata atcacccgaa aaacatatag cccaaaacaa
     5281 tccttccagc aaatcatgcc acccattgcc tggatagacc tgtcacgcaa caccatcccc
     5341 cttcagtcaa aatcctgctg accgctcagg cgcatgcaga agaacattgc cgacgcctat
     5401 accgcagcaa gtttgatcca ggccagaaat cgctgaactt gccgttgccc gcccggccta
     5461 aagcatggga tcgtcaggcc cactgattgt atggtcaaac caattgccag tcggactgcg
     5521 ggtctggcgt cggcagccac acaatatgac aagattttcg ggcatactgg cgcatggccg
     5581 gaaaacccgc caaaccgacg tttcattgcg cacatccgca aggtgcgcgc cgtttgccca
     5641 cactctgacg aacacaggat gccggacatg accatgctca ccccgacgct gtatgccgac
     5701 aacgatctga ccatttacca cgcccaagcg atcaagcaac gctggctgga aggcctgcac
     5761 cagagcgaag gcgacgtcac actggatttg tcgcgggtcg gcgaaatcga caccgctggc
     5821 ctgcaattac tgtggctgct gcgccgggaa gcgcacgcca gtggccggca actgaatatt
     5881 gtcgcggtca gcccggcggt ggctgatctg ctggcgtttt gccgactgac tgaaaacttt
     5941 gccacgccgc gcgcaaccga gcccgaggtg gaggcggcct gaatctcatg gatgaattga
     6001 ctggcgtttt tgtacaggaa agccgcgaac agctggcgga aatggaagcc ggcctgctgg
     6061 cactggaagc caacccggaa gaaaccggcg acatcaacgc catcttccgc gctgcccaca
     6121 ccatcaaggg cggcgctggc gtggtggaat gcagctacct ggaaagcttc acccacaagg
     6181 tggaaaacct gctcgaccaa ctgcgcgatg gccgcttgtc gccgtcggaa atgcgcatca
     6241 gcctgttgct ggactgcgcc gatcacatcg gcctgttgct ggatgcgctg gaacaaggcg
     6301 aaaccgcctt gtccaccagt gtgatcgacg cggatagccg cctgcgtgaa cgactggaac
     6361 tggaaagcac gcccccaggc gcccgcgtca ccgccccccc cgccgaagcg cccgcgcccg
     6421 caccaggcaa cgaagccacc gccgatatct ggcatatctc ggtgcgcttt ggtgaagacg
     6481 tcctgcgcca tggtatggac cccatcgcct ttttgcgctt tttgaccacc ctgggtgaga
     6541 ttcgcgagct gcaaaccctg gccgacacca tgccgcctgc ggcacagatg gatccggaag
     6601 cctgctacct gggttttgaa attcgcctgg aaaccagcgc cggcaagccg gaaatcgagg
     6661 cggtgtttga ttttgtccgt gacgagtgcg aactgcacat cctgccgccg cacagccgct
     6721 cggaagacta cattgccctg atcaacgccc tgccggacga tgagcgtccg ctgctgggtg
     6781 aaatgctgct gcgcgctggc gtactgactg aatccgaact gctgattggc ctgaatagcc
     6841 aggaactggc caaggcttac accgacaaca gcccgccgat tggcgccatt ctggtcgagc
     6901 acaaaagcgt tcgcccggaa gtggtcgagg ccgccatcca gcggcagagc gccattcagc
     6961 aagcccagca gcaaactgcg ccgcccccca gggtgatcga acacgaagaa gtcagcgccc
     7021 atatcgaacc ggtgaccgtg ccggccgtgg tcgctgacgg cgagcaccat ccggccccag
     7081 ccaaggcgcg cgccgccagc agcgaagcgc gcatgctgcg cgtgcaggcg gaaaaactcg
     7141 accggctgat tgatctggtg ggcgagctgg tgattgccgg cgccagcgcc accgaactgg
     7201 cgcgccagag
//
LOCUS       DAOPYX010000146         7198 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_12635_length_7198_cov_7.919222, whole genome shotgun sequence.
ACCESSION   DAOPYX010000146 DAOPYX010000000
VERSION     DAOPYX010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 7198)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7198)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7198
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12635_length_7198_cov_7.919222"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1065)
                     /locus_tag="PKN55_08505"
     CDS             complement(<1..1065)
                     /locus_tag="PKN55_08505"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011136451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbdK family carboxylate-amine ligase"
                     /protein_id="HNX71324.1"
                     /translation="MLEFTPSTPLTLGVELELQIVNRRDYNLTRGASDLMELVHREPA
                     PAGEIKPEITEGMIEISTGIHHDVDAMLAELASIRAVLLRHADRLNLGLCGGGAHPFQ
                     QWPDQRIYPQPRYRLVSELYGYLAQQFTVFGQHIHVGCPDGDHAVQWVQRLAPYMPHF
                     IALSASSPFYQGVDSAFQSSRLNCVNAFPLSGFMPAVADWAEFNAYFDKMRGFGVVES
                     MKDFYWDIRPKPEFGTLEIRVCDTPLTLTRAVQLAAYARALAAWFQAEPRAPEPDQMC
                     MYAFNRFQACRFGLAGNLILADGASCSIAEHVLATLDTVLPYADHPGMRAALVALGEV
                     ARKQDGDAHWLRQQHQARGSL"
     gene            complement(1092..2297)
                     /locus_tag="PKN55_08510"
     CDS             complement(1092..2297)
                     /locus_tag="PKN55_08510"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HNX71325.1"
                     /translation="MPATLTPAAWHTLSLPLNPLLGLGGVLLGGLLGGHWLMRILRVP
                     PATAYVLMGLLLGPAALNLLDRDMLADVQLFIDLAAGMIVFQLGRRIDVRWLLREKRL
                     LASSLLEALCTGAAVAGMLLWLGVNPLAAALAASIALATSPAVLLMAVRDLRAEGQVT
                     ERALHLTALNNVLASLAFAVCLTVLERVPGDGALPWLEPLAQVLGAGALGVAAGALMA
                     WLGQRLPPGDSRQWPLLFAVVVLTLGLAQLWTLAPWVALLACGVAARALDRRESLAEP
                     DVQPLGTVFYLLLFVSLGVSLPPQALGQAWLPALALIGARCAGRLLCLTALARLNALS
                     WRKGAALGLAMQPMSGLALLLADQARLVYPALGGPLLTTLLSAWLLLAVLAPLGVQLA
                     LRLSGDARP"
     gene            2638..3300
                     /locus_tag="PKN55_08515"
     CDS             2638..3300
                     /locus_tag="PKN55_08515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012696284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HNX71326.1"
                     /translation="MQDFNLAALFMLGFLAGGHCLGMCGGIVAALSLQQRQARSHWLL
                     MLAYNTGRLSSYSLVGALVGGIGGLGIASLNIAALQHLLYALANLLLIAMGLYLMGIS
                     AFITQIERLGKPLWRIVQPAMQRLLPVRSPWQGLLVGALWGWLPCGMVYTASLSALAS
                     GSAQQGALTMLAFGLGTLPNLLAMGAFAEALRSLSRQPLVRWTSGGLICALGLWRLAQ
                     VL"
     gene            3350..4201
                     /gene="lgt"
                     /locus_tag="PKN55_08520"
     CDS             3350..4201
                     /gene="lgt"
                     /locus_tag="PKN55_08520"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012697715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HNX71327.1"
                     /translation="MLIHPQFDPIAVSLGPLAVRWYGLMYLLGFVLFMLLGQRRLAQS
                     RGVFTAKMLDDLLFYGVLGVILGGRLGYVLFYKLGDYLADPLAILRVWEGGMAFHGGF
                     LGVVVAMALFARRHGLHFLQVTDFIAPLVPLGLAAGRIGNFINGELWGRVTRPDAPWA
                     MVFPQASAEDTRQALMAPTPEWLTWLASYGGLPRHPSQLYQFALEGVMLFVLLWGFSA
                     RPRPLGQVSALFLLGYGVARFVAEFAREPDNFLGLLALNLSMGQWLSLPMMLAGAWLW
                     QRAARQG"
     gene            4212..5069
                     /locus_tag="PKN55_08525"
     CDS             4212..5069
                     /locus_tag="PKN55_08525"
                     /inference="COORDINATES: protein motif:HMM:TIGR04553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenate ABC transporter
                     substrate-binding protein"
                     /protein_id="HNX71328.1"
                     /translation="MQTGKFWLLALCCAASVAQAAPLLKLSALPDEGASEQQRRYAPL
                     VHYLSAELKQKVQFVTLPDYSSVAEALGRGKLDLAWLGGFSYVQARQLGEVRPIAQRE
                     EDPNHTSVFIVHEDSHAHQLKDLRGKSFAFGAQTSAAGHLMPRWFLQRDGLRPETDFR
                     RLRYSGSHEVTALWVATGKVEAGVLGSAGWQRLVDSDKLDLSEVRVLATTPAYSEFNW
                     SVRPRMSHALRQKLTQALLKLDPRQPAHREVLTALGAPKLIAAQPEQFAALEQAARSA
                     GMLDKKPGN"
     gene            complement(5172..5654)
                     /locus_tag="PKN55_08530"
     CDS             complement(5172..5654)
                     /locus_tag="PKN55_08530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018152658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HNX71329.1"
                     /translation="MLDSRDARLTALAAPLGLDVDAPLVIGGQYCAVLQYGEQLYVSG
                     QVPRVGNQVQVTGAAGDAASLDDARRAAEICALRALILLRQQLGTLDALAQVLKLNVY
                     VQSAPGFTQQSEVADAASALLAQVLGAAGHAARTSVGVAQLPKNATVELDLQAASRVG
                     "
     gene            complement(5647..7113)
                     /locus_tag="PKN55_08535"
     CDS             complement(5647..7113)
                     /locus_tag="PKN55_08535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018748957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HNX71330.1"
                     /translation="MHTPTLYRQWADHYAQAIHSGALPPGERMPSLRDLMARHAVSLS
                     TAVQLCRTLEREGLLEARPRAGYFVRPRRPLASLQEPQPSAPDVAQYVGIHQRVSEVL
                     ALSQRYPAKVNLSGSCGAPSLYPGAALRLATQRALRAQPDIFSQPLAMAGCAPFLQAL
                     AQHALDAQMTLSPDELVVTHGCTEALTLALRAVAQPGEVIAVESPTYYGLLQILESLG
                     LQALEIPASPHTGLSLEALEVAARQCPGLKAVAVTPNLHNPLGCVMPDAHKRQLLRWC
                     QRHDIALIEDNTYTPLYDAGPPLAAIKAWDDSGQVIVCSSLHKILAPGLRLGWIAGGK
                     WHERIAMLKYSQSRPNELLPQLAMTDYLSSGALPRHLSTLKRTLRHQREQMADAVDRL
                     FPAGCRFSLPPGGMSIWVELPGELSSLAVFHAALQQGIRISPGVMFSNAGRVDHCLRL
                     NAGLPYTREVDRAMHTLAQVIEHTLNQAPAGRGKATHA"
BASE COUNT         1233 a         2437 c         2371 g         1157 t
ORIGIN      
        1 cagcgagcct cgggcctggt gctgctgacg cagccaatgg gcatcgccgt cctgcttgcg
       61 cgccacctcg cccaaggcca ccagcgccgc acgcatgccg gggtggtcgg cgtagggcag
      121 cacggtatcc agcgtggcca gcacatgctc ggcaatcgag caagacgcgc cgtcggccag
      181 aatcagattg ccggccagac caaaacggca agcctgaaag cggttgaacg catacatgca
      241 catctggtct ggctcgggcg cgcgcggttc ggcctgaaac caggcggcca gcgcgcgggc
      301 ataggcggcc aactgcaccg cgcgcgtcag cgtcagcggc gtgtcgcaca cgcggatttc
      361 cagcgtgccg aattctggct tggggcggat gtcccagtaa aaatccttca tggattcgac
      421 cacgccaaag ccgcgcatct tgtcaaaata agcgttgaat tctgcccaat cagccaccgc
      481 cggcataaag ccggacagcg gaaacgcatt gacgcaattc agccgcgagg actgaaacgc
      541 actgtccacc ccctgataaa acggcgatga cgccgacagg gcaataaagt gcggcatata
      601 cggcgccagc cgctgcaccc attgcacggc gtggtcgcca tccgggcagc cgacatgaat
      661 atgctggcca aacacggtga actgctgggc cagataacca tacagctcgg acaccagccg
      721 gtagcgcggc tgcggataga tgcgctggtc gggccattgc tggaaaggat gggcgccacc
      781 gccacacaag cccagattca gccggtcggc atggcgcagc agcacggcgc gaatgctggc
      841 cagctcggcc agcatggcgt ccacatcatg gtgaatgccc gtgctgatct cgatcatgcc
      901 ttcggtgatt tctggcttga tttcgccagc gggcgccggc tcacggtgca ccagctccat
      961 caggtcagac gcgccacggg tcaggttgta atcacggcgg ttgacgattt gcagctccag
     1021 ctccacgccc agcgtcagcg gggtggacgg ggtgaattcc agcatgggca aggctccttg
     1081 gtcggatggc ttcagggacg ggcgtcgccg ctcaggcgca gggccagttg cacgcccagc
     1141 ggcgccagca cggccagcag cagccaggca gacaataatg tggtcaacaa ggggccgccc
     1201 agcgccgggt agaccaaccg cgcctgatcg gccagcaata gcgccagccc ggacatcggc
     1261 tgcatggcca gccccagcgc ggcgcccttg cgccagctca gcgcattcag ccgcgccagc
     1321 gccgtcagac acaacagacg gccggcacat cgtgcgccaa tcagcgccag cgccggcaac
     1381 caggcctggc ccagcgcctg cggtggcaga ctgaccccca gcgagacaaa caacagcaga
     1441 taaaacaccg tgcccagcgg ctgcacgtcg ggctcggcca ggctttcgcg ccgatccagc
     1501 gcccgcgccg ccacgccgca ggccagcagc gccacccacg gcgccagcgt ccatagctgg
     1561 gccagcccca gggtaagcac caccacggca aacagcagcg gccattgtcg gctgtcgcct
     1621 gggggcagac gctgacccag ccaggccatc agcgccccgg cggccacgcc cagcgccccg
     1681 gcgcccagca cctgggccag cggttccagc cagggtagcg cgccatcgcc gggcacgcgc
     1741 tccagcacgg tcaggcagac ggcaaaggcc agcgaggcca ggacattgtt cagcgcggtc
     1801 aggtgcaagg cgcgctccgt cacctggcct tcggcccgca gatcacgcac ggccatcaat
     1861 agcaccgcag gagaagtggc cagcgcaatc gacgccgcca gggccgccgc cagcgggttg
     1921 acccccagcc acaacaacat gccggccacc gccgcgcccg tgcacagcgc ttccagcagg
     1981 ctgctggcca gcaggcgttt ttcccgcagc agccagcgca catcaatgcg tctgcccaac
     2041 tggaacacga tcatgccggc cgccagatca ataaacagct gcacatcggc cagcatgtcg
     2101 cggtccagca gattcagcgc ggccgggccc agcagcaagc ccatcagcac ataggcggtg
     2161 gccggcggca cgcgcaagat gcgcatcagc cagtgcccgc ccagcaagcc gcccagcagc
     2221 acgccgccca atcccagcag agggttgagc ggcaggctca gggtgtgcca ggcggcgggg
     2281 gtgagcgtag cgggcatggg cggtctcctg ttatttctcg gcgtcagcct ggggtgatgc
     2341 ggcgggatgg gcgcgagggc gccgaagggt atatggccgg catgggaagc gggcaaaacg
     2401 cgcggcgggg cactgcatgc cgctgttcac tagagccgca gggcggccgc tgagttcccg
     2461 ccgggcagga atgcgcgggt ttttctcagg cgcgttctgc cgcgcatccg tacaataatg
     2521 ccggcgcgct gttgtcgcgc caccggctct gttacccttt gcgcatttcc cgcgctgcac
     2581 catcgccacg gacagcggcc acgccggctg tcgggatttt tcacgcttgg aatctgcatg
     2641 caagacttca atctggccgc gctgttcatg ctcggctttc tggctggcgg ccattgcctg
     2701 ggcatgtgtg gcggcattgt cgccgcgctg tcgctgcaac aacgtcaggc ccgctcgcac
     2761 tggctgctga tgctggcgta taacaccggg cgcttgtcca gctattcgct ggtgggggcg
     2821 ttggtgggcg gcattggcgg gctgggcatt gcttcgttga atatcgctgc gctgcaacac
     2881 ctgctgtatg cgctggccaa tctcttgctg attgccatgg ggctttacct gatgggcata
     2941 tcggccttca tcacccagat cgaacgcctg ggcaaacccc tgtggcgcat tgtgcagccg
     3001 gccatgcaaa ggctgttgcc ggtgcgctcg ccgtggcagg gcttgctggt gggcgcgctg
     3061 tggggctggt tgccgtgcgg catggtgtac accgccagcc tgagcgcgct ggccagcggc
     3121 agcgcccagc aaggcgcctt gaccatgctg gcctttggcc tgggcaccct gcccaatctg
     3181 ctggcgatgg gcgcctttgc cgaagcgttg cgcagcctgt cgcgccagcc gctggtgcgc
     3241 tggacctccg gcgggctgat ttgcgcgctg ggcctgtggc ggctggcgca ggtgctgtga
     3301 gcccgggccg ggtataatcg cgcctttgcc tgtgacttga ggagccggtg tgctgattca
     3361 tccgcagttc gacccgattg ccgtctcgct gggccccctg gccgtgcgct ggtatgggct
     3421 gatgtatttg ctgggttttg tgctgttcat gctgctgggc cagcgccgcc tggcgcaaag
     3481 ccgcggggtg ttcaccgcca agatgctcga cgatttgctg ttttacggcg tgctgggcgt
     3541 gattctgggc ggacggctgg gctatgtgct gttttacaag ctgggggatt acctggccga
     3601 cccgctggcc atcctgcgcg tatgggaagg cggcatggcg tttcacgggg ggtttcttgg
     3661 cgtagtggtg gccatggcgc tgtttgcccg tcgccacggc ctgcattttt tgcaagtgac
     3721 cgactttatc gccccgctgg tgcccctcgg gctggcggcc gggcggattg gcaacttcat
     3781 caatggcgag ctgtggggtc gtgtcacccg gccggatgcg ccgtgggcca tggtgtttcc
     3841 gcaggccagc gccgaagaca cccgccaggc gctgatggcc cccacgccgg aatggctgac
     3901 ctggctggcc agctacggcg gcctgccgcg ccacccttcg cagctgtatc agtttgccct
     3961 ggaaggcgtg atgctgtttg tgctgctgtg ggggttttcc gcgcgcccgc gcccgctggg
     4021 ccaggtgtcg gcgctgttct tgctgggcta tggcgtggcg cgctttgtcg ccgagtttgc
     4081 ccgcgagccg gataattttc tggggctgct ggcgctgaat ctgtcgatgg gccaatggct
     4141 gtcgctgccg atgatgctgg ctggcgcctg gctgtggcag cgcgcggcgc gccagggctg
     4201 aggagcaaag catgcaaaca ggcaaattct ggctgctggc gctgtgctgc gccgccagcg
     4261 tggcgcaagc cgcaccgctg ctgaaactct cggccctgcc cgacgaaggc gccagcgaac
     4321 aacaacgccg ctatgcgccg ctggtgcact acctcagcgc cgagctcaag caaaaagtcc
     4381 agtttgtcac cctgcccgat tacagcagcg tggccgaggc gctggggcgc ggcaagcttg
     4441 atctggcctg gctgggcggc tttagctatg tgcaggcgcg ccagttgggc gaggtgcggc
     4501 cgattgccca gcgtgaagaa gaccccaacc acaccagcgt gtttattgtt cacgaagaca
     4561 gccacgccca tcagctgaaa gacctgcgcg gcaaaagctt tgcctttggc gcgcaaacca
     4621 gcgcggccgg ccacttgatg ccgcgctggt ttttgcagcg cgacggcctg cgcccggaaa
     4681 ccgattttcg gcggctgcgc tattctggct cgcatgaagt caccgcgctg tgggtggcca
     4741 ccggcaaggt ggaggcaggt gtgctgggca gcgccggctg gcagcggctg gtggacagcg
     4801 acaagctgga cctcagcgaa gtgcgcgtgc tggccaccac gccggcgtat tctgaattca
     4861 actggagcgt gcgcccgcgc atgagccatg ccctgcggca aaaactcacc caagccctgc
     4921 tcaagctgga cccgcgccag cccgcccacc gcgaagtgct caccgcactg ggcgcaccca
     4981 aactgattgc cgcccagccc gagcaatttg ccgcgctgga gcaagccgcc cgcagcgccg
     5041 gcatgctgga caaaaaacct ggcaactgag catgtggggt agcgtcatta cccagcgcag
     5101 caatcctggc gcggcgcgcg actgccgaca atcgcagcgg caacagcagc agaagacgcg
     5161 gcgtgccgcg tctaccccac ccgactcgcc gcctgtaaat ccagctccac cgtggcgttt
     5221 ttcggcagct gcgccacgcc caccgaggtg cgcgccgcgt ggccggctgc gcccagcacc
     5281 tgggccagca gcgccgaggc ggcgtcggcc acttcgcttt gctgggtgaa gcctggcgcc
     5341 gactgcacat agacattcag cttcaacacc tgcgccagcg catccagcgt gcccagttgc
     5401 tggcgcaaca aaatcaaggc gcgcagcgcg cagatttccg ccgcgcggcg ggcgtcgtcc
     5461 aggctggcgg cgtcgccagc ggcgccggtc acctgcacct gattgcccac gcgcggcacc
     5521 tggccgctga cgtatagctg ctcgccgtat tgcagcaccg cgcaatactg gccgccgatc
     5581 accagcggcg cgtccacatc cagccccagc ggggcggcca gcgcggtcaa gcgcgcatcg
     5641 cgtgaatcaa gcatgggtgg cctttccgcg cccggcgggc gcctggttca gcgtgtgctc
     5701 aatcacctgc gccagggtgt gcatggcgcg gtccacctcg cgggtgtagg gcaggccggc
     5761 gttcagccgc aggcagtggt ccacccggcc ggcgttggag aacatcaccc ccggcgaaat
     5821 gcggatgccc tgctgcaagg cggcgtggaa caccgccagc gaagataact cccccggcag
     5881 ctccacccag atgctcatgc cgcccggcgg caggctaaag cggcagccag ccggaaacag
     5941 ccggtccacc gcgtcggcca tctgctcgcg ctggtggcgc agcgtgcgct tgagcgtgct
     6001 caggtggcgc ggcagcgcgc cgctgcttaa atagtcggtc atcgccagct gcggcaacag
     6061 ctcattgggc cgcgactggc tgtatttcag catggcgatg cgctcgtgcc acttgccgcc
     6121 ggcaatccag cccagccgca ggccgggggc gagaattttg tgcagcgacg agcagacaat
     6181 cacctgcccg ctgtcgtccc aggccttgat cgccgccagc ggcgggccgg cgtcgtacag
     6241 cggggtgtag gtgttgtctt caatcagggc gatatcgtgg cgctggcacc agcgcagcaa
     6301 ctggcgcttg tgcgcgtccg gcatcacgca gcccagcgga ttgtgcaaat tgggcgtgac
     6361 cgccaccgcc ttcagcccag ggcactgccg cgccgccacc tccagcgcct ccagcgacag
     6421 cccggtgtgc gggctggccg ggatttccag cgcctgcaag cccaggcttt ccagaatctg
     6481 caacagcccg taataagtgg gcgactccac cgcaatcacc tcgcccggct gcgccaccgc
     6541 gcgcaaggcc agggtcagcg cctcggtgca accgtgggtc accaccaact catccggcga
     6601 caaggtcatc tgcgcatcca gcgcatgctg cgccagcgcc tgcaaaaacg gcgcacagcc
     6661 cgccatcgcc agcggctggc tgaaaatatc cggctgcgcg cgcaaggcgc gctgggtggc
     6721 caggcgcaag gccgcgccgg gatacaaact tggcgcgccg cacgaacccg acaaattcac
     6781 cttggccgga taacgctggc tgagcgccag cacctcggac acccgctgat gaatgcccac
     6841 atactgcgcc acatccggcg cgctgggctg cggctcttgc aaactggcca gcggccggcg
     6901 cggccgcaca aaatacccgg cgcgcggccg cgcctccagc agcccttcgc gctccagcgt
     6961 gcggcacaac tgcaccgccg tggacaaact caccgcatga cgcgccatca aatcgcgcaa
     7021 ggacggcatg cgctcacccg gcggcaaggc gccggaatga atcgcctgag cgtaatgatc
     7081 cgcccattgg cggtacaaag tcggagtatg catagccgac atcatgcccg ccccaccgcc
     7141 gcccgggaca gatacagatg agggattttg gcggcggaac agcgggtgtt atggtggc
//
LOCUS       DAOPYX010000147         7146 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_12764_length_7146_cov_8.770131, whole genome shotgun sequence.
ACCESSION   DAOPYX010000147 DAOPYX010000000
VERSION     DAOPYX010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 7146)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7146)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7146
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12764_length_7146_cov_8.770131"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..194)
                     /locus_tag="PKN55_08540"
     CDS             complement(<1..194)
                     /locus_tag="PKN55_08540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX71331.1"
                     /translation="MILKNVASQKMCRGSQQKNRQRAGEQPDAWLMVAGRTGNSIHGP
                     RSARAKNTGFTPVDDRPERNP"
     gene            193..1104
                     /locus_tag="PKN55_08545"
     CDS             193..1104
                     /locus_tag="PKN55_08545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018151572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HNX71332.1"
                     /translation="MMRLTLDALTVLDAIDRNGSFAAAAEELHRVPSAVTYAVQKLEQ
                     DLGVSIFDRRGHRAKLTPVGQALLRDGRHLLNAAVALEQRVRRLASGWETELAIAVSD
                     LLPMAPVLELVRAFDALAAGTQLRLSREVFGGNWDALLEARADLVIGAPGDGPPGAYR
                     ACRMGDIPFVFAVAPQHPLAHQPGPLSLEQVRGYRAVVAADSSRRLPPRSSPLLAGQE
                     TLTVPDMDAKLMAQAAGLGVGFLPRWLAEQAARDGRVRICAVEAPNPPAPAHYAWREH
                     RPGKALSWFLTQLADSQVLARLLGETA"
     gene            1101..>1738
                     /locus_tag="PKN55_08550"
     CDS             1101..>1738
                     /locus_tag="PKN55_08550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX71333.1"
                     /translation="MSALVVLGEAPALGWLAERAFQQGGAVLRGHHWLDAMLLGGARC
                     LLIGEGLFAALPWAEALREGDVVLDVSSDWFELRPAREVRVRELGGHYLELAGPLAPW
                     GGQYGFALAVGGALSAHDAARLWLDRLAPLPGAWSSVGGAGAAAFTRLVAEAFQFAWL
                     GAPELPCAPQAIWMQVRERHLLLAERLLEAAQRYLALHPQEASFAALLASFLV"
     assembly_gap    1739..1838
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1839..2629)
                     /locus_tag="PKN55_08555"
     CDS             complement(<1839..2629)
                     /locus_tag="PKN55_08555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014088346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMIN domain-containing protein"
                     /protein_id="HNX71334.1"
                     /translation="MSVDFDHTRRRLIGAATFTALLSLTRVGHAAGARQIVAVRVWPS
                     STYTRITLESPEPLRFKHFTMKDPERLVVDIDGVQLNSALKSLDGKVGDDDPYIKTAR
                     AGQFNPTTVRVVLDLKTKIEPQVFLLPPVAEYKHRLVIDLYPSASNDPLLALFNDYNQ
                     GKLDDKPAKKPEKTDKHADAQDKDKDSKADKHDKADKADKSEKTEKADKNAARDSSKD
                     SADDSAGKSSGKKSSRNGRPITIVLDPGHGGEDPGAVGPSGAKEKV"
     gene            complement(2593..3075)
                     /gene="tsaE"
                     /locus_tag="PKN55_08560"
     CDS             complement(2593..3075)
                     /gene="tsaE"
                     /locus_tag="PKN55_08560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008952482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HNX71335.1"
                     /translation="MDDSSVWSLRLADEAATLAAGRQLARGLGGGVIVTLHGDLGAGK
                     TTLTRGVLAGLGHVGRVRSPTYTLVEPYELPTLAVYHFDLYRFADPAEWRDAGFAEYF
                     GPHSLCLLEWPEKAQGCLPPADLAIHLDLDGDGRQLTVTALTPRGKQCLSILTTPAAD
                     "
     gene            3079..4155
                     /gene="queG"
                     /locus_tag="PKN55_08565"
     CDS             3079..4155
                     /gene="queG"
                     /locus_tag="PKN55_08565"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011137367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HNX71336.1"
                     /translation="MLPEPAQNRDYPELARQIKAWARDLGFSGATIGRAELPAEVGQR
                     LLDWLAAGHHGEMDYMARHGLLRARPAELVPGALSVVSVRLPYRDPAARDMHAVLADG
                     DKAAIARYALGRDYHKTVRNRLQKLADRLHAAAGPLGYRVFSDSAPLMEVALAAQSGL
                     GWRGKHTLLIHRDEGSFFFLGELLTDLPLPADAPVDAHCGRCTRCLDVCPTQAIVAPY
                     QVDARRCISYLTIELAGAIPEPLRPLIGNRVYGCDDCQLVCPWNRFAVPTAEADFAVR
                     HGLDDASLVSLFAWEADEFAQRMAGSPIYRIGHERWLRNLAVGLGNAPSSPAVTAALL
                     ARREHPSALVREHVAWALARHGVL"
     gene            4213..5025
                     /gene="murI"
                     /locus_tag="PKN55_08570"
     CDS             4213..5025
                     /gene="murI"
                     /locus_tag="PKN55_08570"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009117382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HNX71337.1"
                     /translation="MTALDRRPIGVFDSGIGGLTVVRALMDRLPFEDLIYFGDSARVP
                     YGVKSVATIEHFTQQIAEFLLKRQVKALIIACNTMAAVAADVVRQVAGEVPVLDVIDA
                     GARSAAQVSQQGGIAVIATPTTVNSNAYARAIHHLRPDARVYSQACPLFVPLVEEGWL
                     DHPVTRLTAEEYLKSVLVEDVDTLVLGCTHYPLLKPLLQDVTDNRLIIVDSAVTTAEA
                     TAMRLAELDLLNPQRGMPNYQYFVTDIPLRFQTIGERFLGRSIRQLEMVKLG"
     gene            5063..5629
                     /locus_tag="PKN55_08575"
     CDS             5063..5629
                     /locus_tag="PKN55_08575"
                     /inference="COORDINATES: protein motif:HMM:NF022609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2946 family protein"
                     /protein_id="HNX71338.1"
                     /translation="MDDSVLAAMAKWPNVPEVFGWLSLDARGQWRLRGEPLNHPAMLA
                     FIQRNYTCDRQGRAYFQNGPQRVYVTLEAAPYIARFRQGSWQLHDGEPVHWIGGAFLT
                     PDGSLFIDANRGKLAGVDDHDLDAVLALLRTRQGQPLDEAGWARWLAGEAEYFLHSPP
                     QPPVRLEQAGLDALRERFRIQPDPQADQ"
     gene            complement(5623..>7146)
                     /gene="nadB"
                     /locus_tag="PKN55_08580"
     CDS             complement(5623..>7146)
                     /gene="nadB"
                     /locus_tag="PKN55_08580"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012695492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HNX71339.1"
                     /translation="HRKVGLITKKNLLEGSSTYAQGGIAAVLDDQDSVEAHIQDTLVA
                     GAGLCNPQTTRFIVEHSRAAIDWLIELGVPFTRDGDDSDGVSYQGFHLTREGGHSHRR
                     IIHAADATGEAVTTTLGRKVCAHPNITVLEDHIAIDLITSAKLGETGKRCYGVYALDK
                     LLDEVETITAHHTILASGGAGKVYLYTTNPDTATGDGIAMGWRAGCRVSNMEFIQFHP
                     TCLYHPHAKSFLITEAVRGEGGILRLPDGTRFMPEHDPRGELAPRDVVARAIDFEMKK
                     HGLDCVYLDISYKPASFIQEHFPNIYAHCLDLGIDITQEPIPVVPAVHFTCGGLVTDL
                     NGQTDVENLFAVGEVACTGLHGANRLASNSLLECMVIGRAAAENILKTELASIPPIPD
                     WDDSRVTDPDEEVVLSHNWDELRRSMWDYVGIVRTSKRLERAQRRIDLLKEEINEYYQ
                     HFHVSNDLIELRNLVDTAELIVRSATLRRESRGLHYSRDYPDMLGEAVDTVLTPANY"
BASE COUNT         1080 a         2197 c         2348 g         1421 t
ORIGIN      
        1 ggatttctct ctggccgatc atcaactggc gtgaatcccg tattttttgc ccgggcgctg
       61 cgtgggccgt gtatgctgtt gccggttctg ccggcaacca tcaaccatgc gtctggctgt
      121 tcaccagccc gctgacggtt tttttgttgc gaccccctgc acattttctg gctggccacg
      181 tttttcagga tcatgatgcg cctcaccctc gacgccctta ccgtactgga cgccatcgac
      241 cgcaacggca gttttgccgc cgcggccgag gagctgcatc gcgtgccgtc ggcggtgacc
      301 tatgcggtgc agaagctgga acaagacctg ggcgtgagca tattcgaccg gcgcggccat
      361 cgcgccaagc tcacgccggt cggccaggcc ttgctgcgcg atggacgcca tctgctgaat
      421 gccgcggtgg cgctggagca gcgcgtgcgc cggctggcct ctggctggga gaccgaactg
      481 gccatcgccg tcagcgattt gctgccgatg gcgccggtgc tggagctggt gcgcgccttt
      541 gatgccttgg ccgccggcac gcagctccgc ctgagccgcg aggtgtttgg cggcaattgg
      601 gatgccttgc tggaggcgcg cgctgatctg gtgattggcg cgcccggcga tggcccgccc
      661 ggcgcctacc gcgcctgccg catgggcgat attccttttg tgtttgccgt ggcgccgcag
      721 catccgctgg cgcatcaacc cggcccgctc agcctggagc aggtgcgcgg ctatcgcgcg
      781 gtggtggcgg cggattcctc gcgccgcctg ccgccgcgca gctcaccgct gctggccggc
      841 caggaaaccc tcaccgtgcc ggatatggac gccaagctga tggcgcaggc cgccggcttg
      901 ggcgtgggtt ttctgccgcg ttggctggcc gagcaggcgg cgcgcgacgg ccgtgtccgg
      961 atatgcgcgg tggaagcacc caacccgcca gcgccggcac attacgcctg gcgcgagcat
     1021 cgccccggca aggcgctgag ctggtttctc acgcagttgg ctgattcgca ggtactggcg
     1081 cgtctgctgg gagagacagc atgagcgcct tggtggtgct gggcgaagcg ccagcgctgg
     1141 gctggctggc cgaacgcgcc tttcagcaag gtggtgcggt gttgcgcggt catcactggc
     1201 tggatgccat gttgctgggc ggcgcgcgtt gtctgctgat tggcgagggc ttgtttgccg
     1261 cgctgccgtg ggccgaggcg ctgcgcgaag gcgatgtggt gctggatgtg tccagtgact
     1321 ggtttgagct gcgcccggcg cgtgaggtgc gggtgcgcga gttgggaggg cattatctgg
     1381 agctggccgg ccccttggcg ccgtgggggg ggcaatacgg ttttgccctg gctgttggag
     1441 gcgcgctgag cgcgcacgac gcggcgcggc tgtggctgga tcgcctggcc cccttgcctg
     1501 gcgcctggtc gtcggtgggc ggcgccggcg cggcggcgtt cacccggctg gtggccgagg
     1561 cgtttcagtt tgcctggctg ggcgcgccgg aactgccgtg cgcgccgcag gcgatctgga
     1621 tgcaggtgcg tgagcgccat ttattattgg ccgaacgtct gctggaggcg gcgcagcgct
     1681 atctggcgct gcatccacaa gaggcgtcgt ttgccgcgct gctggccagc tttctggtnn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnac tttttctttg gcgccagacg
     1861 gcccgacggc gccagggtct tcgccgccgt ggccagggtc cagcacaatg gtgatcggcc
     1921 ggccattgcg cgaggatttt ttgccggatg acttgccggc gctgtcatcg gcgctatcct
     1981 tgctgctgtc gcgcgcggcg tttttgtcgg ctttttcggt cttttcgctc ttgtcggctt
     2041 tatctgcctt gtcgtgcttg tcggccttgc tgtccttgtc tttatcctgg gcgtcggcgt
     2101 gcttgtcggt tttttccggt tttttcgccg gtttgtcgtc cagcttgccc tggttgtagt
     2161 cgttgaacag cgccagcagc gggtcgttgc tggccgacgg atacagatca atcaccaggc
     2221 gatgtttgta ttccgccacc ggcggcagca aaaacacctg cggttcgatc ttggttttca
     2281 gatccagcac cacccgcacc gtggtcgggt tgaactggcc ggcacgggcg gtcttgatat
     2341 aggggtcgtc gtcgcccact ttaccgtcca gcgatttcag cgcgctgttc agttgcacgc
     2401 cgtcgatgtc caccaccagg cgctccgggt ctttcatggt gaaatgcttg aagcgcagtg
     2461 gctcggggga ttccagcgtg atgcgggtat aggtgctgga tggccacacc cgcacggcaa
     2521 caatctgccg tgcgcctgcg gcgtggccca cgcgggtcag gctcagcagg gcggtgaaag
     2581 tggccgcgcc gatcagtcgg cggcgggtgt ggtcaaaatc gacagacatt gctttcctcg
     2641 aggggtgagt gcggtcacgg tcagttgacg gccatcgccg tccagatcca gatgaatggc
     2701 cagatcggcc ggcggcaggc agccttgggc tttttccggc cattccagca ggcacaggct
     2761 gtgcgggcca aaatattcgg caaagccggc gtcgcgccat tcggccgggt cggcaaagcg
     2821 atacaggtca aagtggtaca ctgccagcgt gggcaattca tagggctcga ccagggtgta
     2881 agtcgggctg cgcacgcgcc cgacatggcc aagcccagcc agcacgccac gggtcagcgt
     2941 ggtcttgccc gcgcccagat cgccgtgcag ggtgacgatc acgccgccgc ccagcccgcg
     3001 cgccagttgg cggccggcgg ccagggtggc ggcttcgtcc gccaggcgca atgaccaaac
     3061 gctgctatca tccatccaat gcttcctgaa cctgctcaaa accgggatta tcccgaatta
     3121 gcccgacaaa tcaaagcctg ggcgcgcgac ctggggtttt ccggcgccac catcggccgc
     3181 gccgaactgc cggccgaggt gggccagcgc ctgctggact ggctggcggc cggccatcat
     3241 ggcgaaatgg actatatggc ccgccacggc ctgctgcgcg cgcggccggc tgaattggtg
     3301 cccggcgcct tgtcggtggt gtcggtgcgc ctgccctacc gcgacccggc ggcgcgcgat
     3361 atgcacgccg tgctggccga cggcgacaag gcggcgattg cccgctatgc gctggggcgt
     3421 gattaccata aaaccgtgcg caaccgcctg caaaagctgg ccgaccggct gcatgccgcc
     3481 gccgggccgc tggggtatcg ggtgttttcc gacagcgcgc cgctgatgga agtcgccttg
     3541 gccgcccagt cgggcttggg ctggcgcggc aaacatactt tattgatcca tcgtgacgaa
     3601 ggatcgttct tttttctcgg cgaattgctc accgacctgc ccttgccggc cgatgcgcca
     3661 gtggacgcgc attgcgggcg ctgcacccgc tgtctggacg tgtgtccgac ccaggccatc
     3721 gtcgcgccct accaggtgga cgcccgccgc tgtatttctt atctgaccat cgaactggcc
     3781 ggggcgattc ctgagccctt gcgccccttg atcggcaacc gggtgtatgg ctgtgacgac
     3841 tgccagctgg tgtgcccgtg gaatcgcttt gccgtgccca ccgccgaggc ggattttgcc
     3901 gtgcgccatg gcctggacga cgccagcctg gtcagcctgt ttgcctggga ggccgatgaa
     3961 tttgcccagc gcatggccgg cagcccgatt tatcgcatcg gccatgagcg ctggttgcgc
     4021 aatctggcgg tgggcttggg caatgcgccg tccagcccag cggtgacggc ggcgcttttg
     4081 gcccggcgcg aacatccctc ggcgctggtg cgtgaacatg tggcctgggc gctggcccgt
     4141 cacggcgtgc tatgatgacg ccctttgccg gccgcgcgcc cgcgctggcc gccccaaact
     4201 gaagtgagac agatgaccgc cttggaccgt cgccccatcg gggtgtttga ctcagggatc
     4261 ggtggcctga ccgtggtgcg cgcactgatg gatcggctgc cgtttgaaga cttgatctac
     4321 tttggcgaca gcgcccgggt gccctacggg gtgaaatcgg tggcgaccat cgagcatttc
     4381 acccagcaaa ttgccgagtt tctgctcaag cgccaggtca aggcgctgat catcgcctgc
     4441 aacaccatgg ctgccgtggc cgctgacgtg gtgcgccagg tggccggcga ggtgccggtg
     4501 ctggacgtga tcgacgctgg cgcgcgcagc gccgcgcaag tgtcgcagca aggcggcatt
     4561 gccgtgatcg ccacgcccac cacggtcaat agcaatgcct acgcgcgcgc catccatcac
     4621 ttgcgcccgg atgcgcgggt gtattcacaa gcctgcccgc tgttcgtgcc cttggtggaa
     4681 gaaggctggc tggaccaccc ggtgacccgg ctgacggcgg aagagtattt aaagtcggtg
     4741 ctggtggaag acgtggacac cctggtgctg gggtgcacgc actatccgct gctcaagccc
     4801 ttattgcagg acgtgaccga caaccggctg atcattgtcg attcggcggt caccacggcg
     4861 gaggccaccg ctatgcgcct ggctgagctg gatttgctca acccgcagcg cggcatgccc
     4921 aattatcagt attttgtcac cgatattcct ttgcgcttcc agaccattgg cgagcgtttt
     4981 ctcggccgtt cgatccgcca gctggaaatg gtcaagctcg gctgagccca cccgcagcat
     5041 ttcaccacgg agcaaggcgc agatggacga cagtgtattg gcggcgatgg ccaaatggcc
     5101 caatgtgcca gaggtgttcg gctggttgag cctggatgcg cgcggccaat ggcggctgcg
     5161 cggcgagccg ctcaaccatc cggccatgct ggcctttatt cagcgcaact acacttgcga
     5221 ccgtcagggg cgggcgtatt ttcagaatgg gccgcaacgg gtgtatgtga cgctggaggc
     5281 cgcgccgtat attgcccgct ttcggcaggg cagttggcag ctgcacgacg gcgagccggt
     5341 gcattggata ggtggggcgt ttctgacgcc ggacggcagt ctgtttatcg acgccaatcg
     5401 cggcaaactg gctggggtgg atgaccacga tctggacgcg gtgctggcgc tgttgcgcac
     5461 ccggcagggc cagccgctgg atgaagctgg ttgggcgcgc tggctggcgg gtgaggcgga
     5521 gtattttctg cactcaccgc cgcagccgcc agtgcggttg gagcaagccg ggctggacgc
     5581 cttgcgcgag cgctttcgca tccagcccga tccacaagcc gatcaataat tggccggggt
     5641 cagcaccgta tccacggctt cgcccagcat gtccgggtaa tcccggctgt aatgcaggcc
     5701 acggctttca cgacggagcg tggctgaacg cacgatcaat tccgccgtgt ccaccagatt
     5761 gcgcagctca atcaggtcat tgctgacatg aaagtgctgg taatactcgt tgatttcttc
     5821 cttgagcaag tcaatgcggc gctgggcgcg ctccaggcgc ttgctggtgc gcacaatccc
     5881 cacatagtcc cacatcgagc ggcgcagttc gtcccagttg tgcgacagca ccacttcttc
     5941 atccgggtcg gtcacccggc tgtcgtccca gtccggaatc ggcggaatcg acgccagctc
     6001 ggttttcagg atgttctccg ccgctgcccg gccaatcacc atgcattcca gcagcgagtt
     6061 gctggccagc cggttggcgc cgtgcaggcc ggtgcaggcc acttcgccga cggcaaacag
     6121 gttttcgaca tcggtttgtc cgtttagatc agtcaccaga ccgccgcagg tgaagtgcac
     6181 cgccggcacc acgggaatcg gctcctgggt gatgtcgatg cccaaatcca ggcagtgggc
     6241 gtagatattg gggaagtgtt cctgaataaa gctggccggt ttgtacgaga tatccagata
     6301 gacacagtcc aggccgtgtt ttttcatctc gaagtcaatc gcccgcgcca ccacatcacg
     6361 cggcgccagc tcgccgcgcg ggtcgtgctc cggcataaag cgcgtgccgt ccggcaaacg
     6421 cagaatgccg ccctcgccgc gcacggcttc cgtaatcagg aaagacttgg cgtgcgggtg
     6481 gtacaagcag gtcggatgga actggataaa ctccatattc gacacccggc aacccgcccg
     6541 ccaacccatg gcgatgccgt cgccggtggc ggtgtccggg ttagtggtat aaaggtatac
     6601 cttgccggcg ccgccactgg ccaggatggt gtgatgggcg gtgatggtct cgacttcatc
     6661 cagcagctta tccagcgcat acacgccata acagcgcttg ccggtctccc ccagcttggc
     6721 cgaggtgatc aaatcaatgg cgatgtgatc ttccagcacg gtgatattcg ggtgcgcaca
     6781 caccttgcgg cccagcgtgg tggtcaccgc ttcgccagtg gcgtcggcag catgaatgat
     6841 gcggcgatgg ctgtggccgc cttcgcgggt caggtgaaag ccctgatagc tgacgccatc
     6901 gctgtcgtcg ccatcgcggg taaacggcac gcccagctca atcagccagt caatcgccgc
     6961 gcgcgagtgc tcaacaataa agcgcgtggt ttgcggattg cacagcccgg cgccggccac
     7021 cagcgtatct tgtatatgtg cttccaccga atcctgatca tccagcaccg ccgcaatgcc
     7081 gccctgggcg taggtggagc tgccttccag cagattcttc ttggtgatca agcccacctt
     7141 gcggtg
//
LOCUS       DAOPYX010000148         7118 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_12839_length_7118_cov_12.809288, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000148 DAOPYX010000000
VERSION     DAOPYX010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 7118)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7118)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7118
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12839_length_7118_cov_12.809288"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            76..582
                     /gene="def"
                     /locus_tag="PKN55_08585"
     CDS             76..582
                     /gene="def"
                     /locus_tag="PKN55_08585"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003789766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HNX71340.1"
                     /translation="MALLNILHYPDPRLHTRAKPVAQVDDRIRTLIADMGETMYAAHG
                     IGLAATQVNVHERVVVIDLSEEKNQLQAFINPEIVARSEQEEIGEEGCLSVPAVYDKV
                     PRASQVTVRALDANGQPFTVEADGLLAVCLQHELDHLEGKVFVEYLSPLKQNRIRTKL
                     KKQEKRTL"
     gene            603..1517
                     /gene="fmt"
                     /locus_tag="PKN55_08590"
     CDS             603..1517
                     /gene="fmt"
                     /locus_tag="PKN55_08590"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011137810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HNX71341.1"
                     /translation="MKLIFAGTPEFAASALQALIDAGHDIALVLTQPDRPAGRGMKLK
                     ASPVKTLALAHGLPVSQPLTLKTAEAQAEIAAVGAEVMVVAAYGLILPKAVLEMPTRG
                     CLNIHASLLPRWRGAAPIHRAIEAGDAETGVTIMQMDVGLDTGDMLLTVREPIHADDS
                     TGSLHDRLAAQGAQAMVAALAQLDALSPQPQPAEGVTYAEKIGKAEAQLDWSLPAEVL
                     HRKIRAFNPFPGASTLAGGEALKIWQAQLADGAGAPGEVLAADADGVRIACGSGALLA
                     TQLQAAGGKRLAAREFLAGHSLPARLGE"
     gene            1780..3030
                     /gene="rsmB"
                     /locus_tag="PKN55_08595"
     CDS             1780..3030
                     /gene="rsmB"
                     /locus_tag="PKN55_08595"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018748121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HNX71342.1"
                     /translation="MQTIQTLAAHILDTVLSGKNLTDTLATAWRQHPALAPAERAAVM
                     DITHGALRHYGWLRGALDKLLSRPLTDSHIDRLLIVALYQLEHTQAAPYAIVDHAVRV
                     ASAHEGGRLKGLTNAVLRNFQRRHDELAAALRREPTAFWNHPDWWVGAVQQAYPKDWK
                     AILTASNQHPPMTVRVNRRRLSVADWLTRLQEAGLEGVVLEGDAVRLVKPVPVDKLPG
                     FAEGDVSVQDAGAQLAADWLEVADGMRVLDACAAPGGKTCHLLERAQLDLTAVDNDAH
                     RLQRVQSNLTRLGLSARCLTGDASRPKAWWDGQMFDRVLADVPCSASGVARRHPDIKW
                     TRRPTDIPSLGRQQGALADALWALVAPGGKMLYATCSIFPTENRRQIDAFLTRHVNAE
                     LERDIQLLPDENHDGFYYALIRKT"
     gene            3053..3613
                     /locus_tag="PKN55_08600"
     CDS             3053..3613
                     /locus_tag="PKN55_08600"
                     /inference="COORDINATES: protein motif:HMM:NF025692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4390 domain-containing protein"
                     /protein_id="HNX71343.1"
                     /translation="MALAISLWLVALPSRAEGINPRLASLALQDGYLVANTRFAIQLN
                     PTLSDALDEGVPLAFRLQFRLNRPRLYAWWQQLSGGFEPTAEQQYKLSFHSLTQTWRV
                     SVGGALYKSYPTLNDALAAIGAIRGWRVLGKAAVGKSRLSEFAGEVRLSLDISQLPRP
                     FQLSALGATDWKLESDWTPMALEGEH"
     gene            3613..5733
                     /locus_tag="PKN55_08605"
     CDS             3613..5733
                     /locus_tag="PKN55_08605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012695550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNX71344.1"
                     /translation="MRVWLASTALLGLILLYLLAISTGNTSRLAEYYWEIFSLTSLLT
                     VGLIGFIGRQLWQIRQKIQAKVFGAKLTLRMVAMFALVAILPGILMFTVSVQFLTKSI
                     ESWFDVRVEAGLDRGLSLGRNALDYLLTDLDYKAQQVARELNGAPALSATLKLPALRN
                     QLGVHEIAVFDRHGQLIAAAGEPGAGAEPPQAPSREVLERIANGQPFRTTETLAGRGM
                     VLRVALPAYPLGFNNERLTVQLSQPAPRQIAEDAELVDATRSEYRQLALSREGLKRFY
                     RLTLALTLVVTLLGAVSLGFYLSDRLSAPLGALADGTRAVTQGDYSRQHPIYSRDELG
                     VLTAMFNRMTKQLAEARAQTDASRAELEANNAYLESILVNLSAGVVAFDGHWRLTAAN
                     VSAERILGVGIDELAEHPLPDWPRLAPRLSPLVDATLAHADDAPGDDWQAEVAMEGER
                     LGQTLLARGVRLPEKTGHGFVMVFDDITDLSRAQRDAAWGEVAKRLAHEIRNPLTPIQ
                     LSAERLQMKLADKLAPNDADMLARATRTIVEQVNALKGMVEAFRDYARAPKTQLARLD
                     LANLMREVLALYESNPAVRASLPAQPLWIAGDATLLRQVLHNLLVNAQDAVLDAEQPM
                     IHISAQQENDAVVISLEDNGPGFAAEILPRAFEPYVTSKAKGTGLGLAVVKKIIEEHH
                     GSITVGNVTPHGARICVTLPCPEA"
     gene            5733..7001
                     /locus_tag="PKN55_08610"
     CDS             5733..7001
                     /locus_tag="PKN55_08610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008954825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNX71345.1"
                     /translation="MRSSDILIVDDEVGIRELLSEILQDEGYTVALAENAEVARQLRN
                     QTRPALVLLDIWMPDCDGVTLLKEWAKAGQLNMPVVMMSGHASIDTAVEATRIGAFDF
                     LEKPIALQKLLTTVQRAMKYADMQGNQALNLDRLGKSPVVHELKRALERVVPVKSPIL
                     LTGEPGVGFELVARFFHQANTPWVTPNKPEQLADAPLELLQKANNGVLYLPDIGSQPR
                     RAQQGLLFLLSKLERYNVRLVCSCSRPLQELLVDPACDNRLLTALSSVIVPIPSLREH
                     ADDIVELAQQLLAELIDNKQVPAHRLTTAALNALRQYDWPGNLEQLRSIIKSLALTAD
                     SDDIDAPEVNKVLAQFRQEKPPEESGFDFNLPLRELREQLERRYFEYHIGLENGNMSR
                     VAQKVGLERTHLYRKLKQLGVKFSRRGAED"
BASE COUNT         1281 a         2331 c         2293 g         1213 t
ORIGIN      
        1 tttttgccgc catccggcgt cgggctataa tgccgcatcc gggcgcatcg cccccattct
       61 ttcccgaaca cacccatggc cctgctcaat atcctgcatt accccgaccc ccgtctgcat
      121 acccgcgcca agccggtggc ccaggtggac gaccgcatcc gcaccctgat tgccgacatg
      181 ggcgaaacca tgtacgccgc ccacggcatc ggcctggccg ccacccaggt gaatgtgcac
      241 gagcgcgtgg tggtgattga tctgtctgaa gaaaaaaacc agctgcaagc gtttatcaac
      301 ccggaaatcg tcgcccgctc ggagcaagaa gaaatcggcg aagaaggctg cctgtcggtg
      361 ccggcggtct acgataaagt gccgcgcgcc agccaggtga cggtgcgcgc gctggacgcc
      421 aacggccagc cattcaccgt cgaggccgat ggcctgctgg cggtgtgcct gcaacacgag
      481 cttgaccacc tggagggcaa ggtgtttgtg gaatacctgt cgccgctcaa gcaaaaccgc
      541 atccgcacca aactgaaaaa acaggaaaag cgcaccctgt aagggcgcag gagagccgcc
      601 cgatgaaact gatttttgcc ggcacgccgg aattcgccgc cagcgcgctg caagcgctga
      661 ttgacgccgg ccacgacatc gccctggtgc tcacccaacc cgaccgcccg gccgggcgcg
      721 gcatgaagct gaaagccagc ccggtgaaaa ccctggcgct ggcgcacggc ctgccggtca
      781 gccagccgct gacgctgaaa accgccgaag cccaggcgga aatcgccgcc gtcggcgccg
      841 aagtgatggt ggtggccgcc tacgggctga ttctgcctaa agccgtgctg gagatgccca
      901 cgcgcggctg cctgaacatc cacgcctcgc tgctgccgcg ctggcgcggc gcggcgccga
      961 ttcatcgcgc catcgaggcc ggcgacgcag aaaccggcgt caccatcatg caaatggacg
     1021 tcggcctgga cactggcgac atgctgctga ccgtgcgcga gcccatccac gccgacgaca
     1081 gcaccggcag cctgcacgac cgcctggccg cccaaggcgc gcaagccatg gtggcggcgc
     1141 tggcccagct ggacgcgctg agcccgcagc cgcagccggc cgagggcgtg acctacgccg
     1201 aaaaaatcgg caaggccgaa gcccagctgg attggtcgct gccggcggaa gtgctgcacc
     1261 gcaaaatccg cgccttcaac cccttccccg gcgccagcac cctggccggg ggcgaggcgc
     1321 tgaaaatctg gcaagcccag ctggccgacg gcgctggcgc gccgggtgag gtgctggcag
     1381 ccgacgccga cggcgtgcgc attgcctgcg gcagcggcgc gctgctggcc acccagctgc
     1441 aagccgctgg cggcaaacgg ctggccgcac gggagtttct ggcggggcac agcctgccgg
     1501 cgcggctggg cgagtaaaaa gcgcgcacaa tagcgcctgg ctgggccgga cttgttgccc
     1561 tgtctgcggt cactcgccta gccaggatcg ctgtgcggcg ttttgtcagc gtttctccgg
     1621 cccgctggcc aaacccgcct gctttgttcc gtaccagggg atatccgggt ttgttgtgcg
     1681 ggtttggcgg cgaaaccgca cgccctggtc cggtttcggc tacaatccgg ctgattcaac
     1741 atccccgccg ccacgcgttg ccgtggcggt tttcacatca tgcaaaccat ccagaccctc
     1801 gccgcccata ttctcgacac cgtgctgtcg ggcaagaacc tgaccgatac gctggccact
     1861 gcctggcgtc agcaccctgc gctggccccg gccgagcggg cggcggtgat ggacatcacc
     1921 cacggcgcac tgcgtcacta cggctggctg cgcggcgcgc tggacaagct gctgagccgg
     1981 ccgctgactg acagccacat tgaccgtctg ctgattgttg ccctgtatca gctggagcac
     2041 acccaggccg cgccctacgc cattgtggat cacgccgtgc gcgtggccag cgcccacgaa
     2101 ggcggccggc tgaaggggct gaccaatgcc gtgctgcgca actttcagcg ccgtcacgac
     2161 gaactggccg ccgcgctgcg ccgcgagccg acggccttct ggaatcaccc ggattggtgg
     2221 gtgggcgcag tgcagcaggc ttacccgaaa gactggaaag ccattctcac cgccagcaat
     2281 cagcacccgc cgatgaccgt gcgcgtcaat cgccgccgcc tcagcgtggc cgactggctg
     2341 actcgcctgc aagaggccgg cctggaaggt gtggtgctgg agggcgatgc cgtgcgtctg
     2401 gtcaagccgg tgccggtgga taaattgccc ggttttgccg agggcgatgt gtcggtgcag
     2461 gatgccggcg cacaactggc cgccgactgg ctggaagtgg ccgacggcat gcgcgtgctg
     2521 gacgcctgcg ccgcgccggg gggcaaaacc tgccatctgc tggagcgcgc ccaactggat
     2581 ctgaccgcgg tggataacga cgcccaccgc ctgcaacggg tgcaaagcaa cctcacccgt
     2641 ctgggcctga gcgcgcgctg tctgaccggc gatgccagcc ggcccaaggc ctggtgggat
     2701 ggccagatgt tcgaccgcgt gctggccgat gtgccgtgct cggcctcggg cgtggcgcgc
     2761 cgccacccgg acatcaaatg gacgcgccgg ccgactgaca tcccctcgct gggccgccag
     2821 cagggcgcgc tggccgatgc gctgtgggcg ctggtggcgc cgggcggcaa gatgctgtac
     2881 gccacctgct cgatctttcc gactgaaaac cgccgtcaga tcgacgcctt tctgactcgg
     2941 cacgtcaatg ccgagctgga acgcgacatt cagctgttgc ccgacgaaaa ccatgatggc
     3001 ttttattacg cgctcatccg caaaacctga gcccgcccgc tggctggttt ggttggcgct
     3061 ggccatcagc ctgtggctgg tggcgctgcc cagtcgcgcc gagggcatta atccccggct
     3121 ggccagcctg gccttgcagg atggctatct ggtggccaat acccgctttg ccatccagct
     3181 caaccccacc ctcagcgacg cgctggacga aggcgtgccg ttggcgtttc gcttgcagtt
     3241 tcgcctgaac cgcccccgcc tgtatgcctg gtggcagcag ttgagcggcg gttttgagcc
     3301 caccgccgag cagcaataca aattgtcgtt tcacagcttg acgcaaacct ggcgggtgtc
     3361 ggtgggcggc gcgctgtaca aaagctatcc cactttgaac gatgccctgg ctgccattgg
     3421 cgccatccgc ggctggcggg tgctgggcaa ggccgcggtg ggcaaaagcc ggctgagcga
     3481 gtttgctggc gaagtgcggc tgagcttgga catcagccag ttgccacgtc cgtttcagct
     3541 gtcggcgctg ggcgccaccg actggaaact ggaatccgac tggacgccga tggcgctgga
     3601 aggagagcac tgatgcgcgt ctggctggct tctaccgccc tgttggggct gattctgctg
     3661 tatctgctgg cgatatccac cggcaatacc tcgcgtctgg ccgagtatta ctgggaaatc
     3721 ttttcgctga ccagcttgct gaccgtgggc ctgattggct ttatcggccg ccagctgtgg
     3781 caaatccgcc agaaaatcca ggccaaggtg tttggcgcca agcttaccct gcgcatggtg
     3841 gcgatgtttg ccctggtggc catcttgccc ggtattttga tgttcaccgt gtcggtgcag
     3901 tttctcacca agagcatcga gagctggttt gatgtgcgcg tggaggccgg gctggatcgc
     3961 ggcctgagcc tggggcgcaa tgcgctggat tatctgctga ccgatctgga ctacaaagcc
     4021 cagcaagtgg cgcgcgagct gaacggagcc ccggcgctga gcgccacgct caagctgccg
     4081 gccttgcgca atcagctggg cgtgcatgaa atcgccgtgt tcgaccgtca cggccagctg
     4141 atcgccgctg cgggcgagcc aggcgcgggc gccgaaccgc cgcaagcgcc ctcgcgtgaa
     4201 gtgctggagc gcattgccaa tggccagccg tttcgcacca ccgaaaccct ggccggacgc
     4261 ggcatggtgc tgcgcgtggc cttgccggcc tacccgctgg gctttaacaa tgagcggttg
     4321 accgtacaac tgagccagcc cgcgccccgg cagattgccg aagacgccga gctggtggac
     4381 gccacccgca gcgaataccg ccagttggcc ttgtcgcgcg aggggctcaa gcgcttttac
     4441 cggctgaccc tggccctgac tctggtggtg accctgctgg gggcggtgag tttagggttt
     4501 tatctgtctg accgcctgtc tgccccactg ggtgcgctgg ccgacggcac gcgggcggtg
     4561 acccagggcg attattcgcg tcagcatccg atttacagcc gcgacgagct gggtgtgctc
     4621 accgccatgt tcaaccgcat gaccaagcaa ctggccgagg cacgcgcgca aaccgacgcc
     4681 agccgcgccg agctggaagc caacaacgcc tacctggaga gcatcctggt caacctcagc
     4741 gccggcgtgg tggcgtttga cggccactgg cggctgactg cggccaatgt ctcggccgag
     4801 cgcattctgg gcgtgggcat cgacgagctg gccgaacacc cgctgcccga ctggccacgg
     4861 ctggcgccac ggctaagccc gctggtagac gccacgctgg cgcacgcgga cgatgcgccg
     4921 ggcgacgact ggcaggccga agtcgccatg gaaggcgagc ggctggggca aaccctgctg
     4981 gcgcgcggcg tgcgcctgcc agaaaaaacc ggccatggct ttgtgatggt gtttgacgac
     5041 atcaccgacc tgtcgcgcgc ccagcgcgac gccgcctggg gcgaggtggc caagcggctg
     5101 gcgcatgaaa tccgcaaccc gctgacgccg attcagctgt cggccgagcg gctgcaaatg
     5161 aagctggccg acaagctggc gcccaacgat gctgatatgc tggcgcgcgc cacccgcacc
     5221 attgtcgagc aggtgaacgc gctcaagggc atggtcgagg cgttccgcga ttatgcccgc
     5281 gcgcccaaga cccaactggc gcgactggac ctggcaaacc tgatgcgcga ggtgctggcg
     5341 ctgtacgaat ccaacccggc ggtgcgcgcc agcctgccgg cgcagccctt gtggattgcc
     5401 ggcgacgcca ccttattgcg gcaggtgttg cacaatctgc tggtcaatgc ccaggacgcg
     5461 gtgcttgacg ctgaacagcc gatgattcac attagcgccc agcaagaaaa tgatgcggtc
     5521 gtcatctctc tggaggacaa tggcccaggg tttgctgcag agatcctgcc gcgtgccttc
     5581 gagccctacg tcaccagcaa ggccaagggc accggcctgg gcctggcggt tgtaaagaag
     5641 atcattgagg aacaccatgg cagcattacc gttggcaacg tcaccccgca cggcgcccgc
     5701 atttgcgtta cgctgccttg cccggaggca tgatgcgaag tagcgatatt ctgatcgtcg
     5761 atgatgaagt cggcattcgc gagctgctgt cggaaatcct tcaggacgaa ggctacaccg
     5821 tggcgctggc ggaaaacgcc gaagtggcgc gccagctgcg caaccaaacc cgcccggcgc
     5881 tggtattgct ggacatctgg atgcccgatt gcgatggggt gaccttgctc aaggaatggg
     5941 ccaaggccgg gcagctgaac atgccggtgg tgatgatgtc cggccatgcc agcatcgaca
     6001 ccgccgtcga agccacccgc atcggcgcgt ttgattttct ggaaaaaccc atcgccctgc
     6061 aaaaactgct caccacggtg cagcgggcga tgaaatacgc cgatatgcag ggcaatcagg
     6121 cgctgaatct ggatcgcctg ggcaaaagcc cggtggtgca cgagctcaag cgcgcgctgg
     6181 agcgcgtggt gccggtcaaa tcgccgattt tgctcactgg cgagccaggc gtggggtttg
     6241 agctggttgc ccgctttttt catcaggcca acaccccatg ggtgacgccg aacaagcctg
     6301 agcaactggc cgacgcgccg ctggaactgc tgcaaaaggc caataacggc gtgctgtatc
     6361 tgcccgacat cggcagccag ccgcgccgcg cccaacaggg cttgttgttc ctgctgtcca
     6421 agctggagcg ctacaacgtg cgcctggtgt gttcgtgcag ccggccgctg caagagctgc
     6481 tggtcgatcc cgcctgcgac aatcgcctgc tgaccgcgct atccagcgtg attgtgccca
     6541 tccccagcct gcgcgagcac gctgacgaca tcgtcgagct ggcccagcag cttttggccg
     6601 agctgatcga caataaacag gtgccggcgc accggctgac cactgccgcg ctgaatgccc
     6661 tgcgccagta cgactggccg ggcaatctgg agcaactgcg cagcatcatc aaaagcctgg
     6721 cgctcaccgc cgacagcgac gacatcgacg cgcccgaagt caacaaggtg ctggcgcagt
     6781 tccgccagga aaaaccgcca gaagaatccg gctttgactt taatctgccg ctgcgcgagc
     6841 tgcgcgagca gctggagcgg cgctacttcg aatatcacat tggcctggaa aacggcaata
     6901 tgagccgggt ggcgcaaaaa gtcggcctgg agcgcaccca tttgtatcgc aagctcaagc
     6961 aattgggcgt caaattcagc cggcgtggcg ccgaagactg atctcgcacc cggcgtgcgt
     7021 tctgcgcgcc gggcacatca ctgtggcaaa tttgcccatc tgcaaaaaaa attggccaaa
     7081 agccggctaa cgtcgtattt ctacgctggt ttttctgg
//
LOCUS       DAOPYX010000149         7065 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_12960_length_7065_cov_8.576034, whole genome shotgun sequence.
ACCESSION   DAOPYX010000149 DAOPYX010000000
VERSION     DAOPYX010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 7065)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7065)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7065
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12960_length_7065_cov_8.576034"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..374)
                     /locus_tag="PKN55_08615"
     CDS             complement(<1..374)
                     /locus_tag="PKN55_08615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HNX71346.1"
                     /translation="MTPVLAFDIETIPDIAGLRLLHGLPDTDVSDADVAEYAFQRQRQ
                     KNGSDFLPHHLHRVVAISCVLRWGERIHVSTIGQPGDSEARMLEMFFALIEKYTPQLV
                     SWNGGGFDLPALHYRALIHGIAA"
     gene            complement(510..2201)
                     /gene="recJ"
                     /locus_tag="PKN55_08620"
     CDS             complement(510..2201)
                     /gene="recJ"
                     /locus_tag="PKN55_08620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012697865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HNX71347.1"
                     /translation="MTAIAVRAAPEDARLALQAQGLPPLFSRLFAARGVARAEQLDYS
                     LKRLLPYHTLKDIGPAAVRLADAIARQERMLVVADYDADGATACALAVRGLRRLGGVV
                     EFIVPNRFEYGYGLTPEIVELAAQRRPDLLITVDNGVASVAGVQAARRHGMDVLITDH
                     HLAGDELPDALIVNPNQPGCQFPSKHLAGVGVMFYVLMALRAELRQRGAFAHGDEPNL
                     ADWLDLVALGTVADVVKLDDNNRILVEQGLRRMRAGRACAGVNALFAASGRQARRAST
                     VDLGFMLGPRLNAAGRLDDMSLGIACLLADDGAHALALAGELDRLNKERRHIEAGMQE
                     EALAHLAGVDPQGRSTLCLYREDWHQGVVGIVASRLKDRFHRPTLVFAPGDEGEVKGS
                     GRSIPGFHLRDALDRVAKQQPDLLRKFGGHAMAAGVTLAEADLPRFIAAFEAVAQDWL
                     TPAQLTRTIETDGSLAVRDIAMDTADQLAAQVWGQGFPPPSFHDQFRVLDQRRVGERH
                     LKLKLGRDGGEFDAMLFNCPDWLPARVDVVYQLNVNEWRGERNLQLMVDNWQPAA"
     gene            complement(2198..3214)
                     /locus_tag="PKN55_08625"
     CDS             complement(2198..3214)
                     /locus_tag="PKN55_08625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX71348.1"
                     /translation="MPLSHLTLVVPDLLWADPDLRREATRELPLPALGWLLGRGQARA
                     QAQSASACLAELFAAPADLPVARALARADGLAADDGHWLCADPVHLRVEQDYLLLADA
                     RMFALNQAEADALVATLNSHFGQDGLCFYAPTPNRWYLRLDAASAAEFTPLAEAAAQN
                     IDFHLPRGERASYWRHMLNELQMLLFNHPVNEAREQAGAPVINSVWPWGQGDAALRAP
                     AAQLWADSPLPLALASQAGARAASAPAQLDAAWLAAQSGEALIWLDTLSGVLRYQDGY
                     GWRQRLQALEADYFAPLAQALKTGQLGRLTLRSPGEAGFCAQLGAAERWKFWRSPRTL
                     AELT"
     gene            complement(3228..4451)
                     /gene="tyrS"
                     /locus_tag="PKN55_08630"
     CDS             complement(3228..4451)
                     /gene="tyrS"
                     /locus_tag="PKN55_08630"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018150589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HNX71349.1"
                     /translation="MTAPAPLSAELAAALETIRRGADELLIEAELIAKLKENRPLRIK
                     AGFDPTAPDLHLGHTVVLTKMRQLQDMGHHIMFLIGDFTGMIGDPTGKNTTRPPLTPE
                     QVAINAKTYQEQVYKILDPAKTEICFNSTWLNQLGAGGMLKLAASTTVARMLERDDFK
                     KRFGNNQPISLHEFMYPLMQGYDSVAMQADIELGGTDQKFNLLMGRMLQEQHGQKPQC
                     VIMMPLLEGLDGVNKMSKSLGNYIGISEAANDIFGKVMSVSDTLMWRYYELLSLRPLA
                     EIEQFKADIAAGRNPRDVKVLLAQELVARFHSQAAAEAALADFEARFKQNAIPDDLPE
                     VTVQTGEGGVLAIGSVLKEAGLVASTSEAMRMIDGGGVKADGEKIEDKKLQLAAGATL
                     VLQVGKRKFAKVTLV"
     gene            complement(4622..5851)
                     /locus_tag="PKN55_08635"
     CDS             complement(4622..5851)
                     /locus_tag="PKN55_08635"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011135542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HNX71350.1"
                     /translation="MSDVKKVVLAYSGGLDTSVILKWLQDTYQCEVVTFTADLGQGEE
                     LEPARAKALQFGIKPENIFIDDLREEFVRDFVFPMFRANTVYEGEYLLGTSIARPLIA
                     KRLIEIANQTGADAISHGATGKGNDQVRFELGAYALKPGVKVIAPWREWDLLSREKLL
                     AYAETNGIPIDMKHRNGGSPYSMDANLLHISFEGRHLEDPAAEAEESMWRWTVSPEQA
                     PDAAEYLDVEFAKGDIVALNGVAMSPAQVLTKLNELGGKHGIGRLDLVENRYVGMKSR
                     GCYETPGGTIILKAHRAIESITLDREVAHLKDDLMPRYASMIYNGYWWSPERLALQAL
                     IDHTQQTVNGWVRLKLYKGNVIVVGRDSKTDSLFDPTIATFEDDAGAYDQRDAGGFIK
                     LNALRMRIAANLKAKKG"
     gene            complement(5915..6844)
                     /gene="argF"
                     /locus_tag="PKN55_08640"
     CDS             complement(5915..6844)
                     /gene="argF"
                     /locus_tag="PKN55_08640"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014087636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HNX71351.1"
                     /translation="MKPARHYLQFSDLTRDEYLHLFERSRILKRRHYDGELYRPLVGK
                     VMSMVFEKNSTRTRASFEAGMAQMGGHAMFLDSKSSQLGRGEPIEDTARVLSRMSDII
                     MIRTFEQALVERLAAFSRAPVINGLTNEYHPCQVLADLFTWVEQRGPISGRTVAWIGD
                     GNNVARTWLQAAKLLGFKFRIASPKGYELTVLDAQDYGPDVYEVCDNPLDAARGADIV
                     TTDVYTSMGFEAEAEARHRAFAGYGVTAAVMAAAKPDAMFMHCLPAHRGEEVDAEVID
                     GPNSVVWDEAENRMHVQKALVEFLLLGRVADKK"
BASE COUNT         1169 a         2281 c         2356 g         1259 t
ORIGIN      
        1 gcggcaatgc cgtgaatcag cgcgcggtag tgcagcgccg gcaggtcaaa gccgccgccg
       61 ttccagctga ccagctgtgg ggtgtatttt tcgatcaggg caaaaaacat ctccagcatg
      121 cgcgcttcgc tgtcgccggg ctggccgatg gtgctgacat gaatgcgctc gccccagcgc
      181 agcacgcagg aaatcgccac cacccggtgc aggtgatgcg gcaaaaaatc actgccgttt
      241 ttttgccgct ggcgctgaaa ggcgtattcg gccacgtcgg cgtcgctgac gtcggtatcg
      301 ggcagcccgt gcagcaggcg caggccggca atatccggga tggtttcgat gtcaaaggca
      361 agaacggggg tcaaggcggc gctccgcgaa ggtggcaatc tgccattgta gggattcgga
      421 gggaagggcg cgacagcggc aggcggacgg ctgcaccagg tggaggggcc cccttggtgc
      481 atgccacatt ctttggccaa gagcagtgct tatgccgccg gctgccagtt gtccaccatc
      541 agctgcaaat tgcgctcgcc gcgccattcg ttgacattca gctgatacac cacgtccacc
      601 cgcgccggca gccagtccgg gcagttgaac agcatggcgt caaattcgcc gccgtcgcgg
      661 cccagcttga gcttcagatg gcgttcgccc acccggcgct gatccagcac gcgaaactgg
      721 tcatgaaacg acggcggcgg aaagccctgg ccccacacct gcgctgccag ttggtcggcg
      781 gtgtccatgg cgatgtcgcg caccgccagg ctgccatcgg tttcgatggt gcgcgtcagc
      841 tgcgccgggg tgagccagtc ttgcgccacc gcttcaaagg cggcgataaa gcgcggcaaa
      901 tccgcctcgg ccagggtcac cccggcggcc atggcgtggc cgccaaactt gcgcaataaa
      961 tccggctgct gcttggccac ccggtccagc gcgtcgcgca gatgaaaacc cggaatcgac
     1021 cgccccgagc ccttcacctc accctcgtcg ccaggggcaa acaccagcgt aggccgatga
     1081 aaacggtctt tcagccggct ggcgacaatg cccaccacgc cctggtgcca gtcttcccga
     1141 tacaaacaca gcgtcgagcg gccttgcggg tccaccccgg ccaggtgggc cagggcttct
     1201 tcctgcatgc cggcctcgat atggcggcgc tctttgttca gccggtccag ctcgccggcc
     1261 agcgccagcg cgtgcgcgcc gtcgtcggcc agcaggcagg caatgcccag gctcatgtcg
     1321 tccagccggc cagcggcgtt caacctcggg ccgagcataa agcccaggtc caccgtgctg
     1381 gcgcggcgcg cctggcggcc gctggcggca aacagcgcgt tcaccccggc gcaggcgcgg
     1441 ccagcgcgca tgcggcgcag gccttgctcg accagaatgc ggttattgtc gtccagcttc
     1501 accacgtcgg cgacggtgcc cagcgccacc aaatccagcc agtcggccag attgggctcg
     1561 tcgccgtggg caaaggcgcc gcgctggcgc agctcggcgc gcagcgccat cagcacgtaa
     1621 aacatcaccc ccaccccggc caggtgcttg ctgggaaact gacagccagg ctggttgggg
     1681 ttgacgatca gcgcgtcggg cagttcgtcg ccggccaggt gatggtcggt gatcagcacg
     1741 tccatgccgt ggcggcgggc cgcctgcacg ccggccacgc tggccacgcc gttgtccacg
     1801 gtgatcagca agtccgggcg gcgctgggcg gccagttcga cgatttccgg ggtgaggccg
     1861 tagccgtatt caaagcggtt ggggacgatg aattccacca cgccgcccag ccggcgcagg
     1921 ccgcgcaccg ccagcgcgca ggcggtggcg ccgtcggcgt cgtagtcggc caccaccagc
     1981 atgcgttctt gccgggcgat ggcgtcggcc agccgcacgg cggccgggcc gatgtctttc
     2041 agcgtgtggt agggcagcag gcgcttgagg ctgtaatcca gctgctcggc gcgcgccacg
     2101 ccacgggcgg cgaacagccg cgaaaacagc gggggcaggc cctgggcctg cagggcaagg
     2161 cgggcgtctt ctggcgccgc gcgcacggca atggcggtca tgtcagctcc gccagggtgc
     2221 gcgggctgcg ccagaatttc cagcgctcgg ccgcgcccag ctgggcgcaa aacccggcct
     2281 cgcccggcga gcgcagcgtc agccggccca gctggccggt tttcagcgcc tgggccagcg
     2341 gggcaaagta atccgcctcc agtgcttgca ggcgctgccg ccagccgtag ccatcctgat
     2401 agcgcagcac gccgctgagc gtgtccagcc agatcagcgc ctcgccggac tgcgccgcca
     2461 gccaggcggc gtccagctgg gccggggcgc tggcggcgcg cgcgccggcc tggctggcca
     2521 aggccaaggg cagcgggctg tccgcccaca gctgcgcggc cggcgcgcgc aaggcggcgt
     2581 cgccctggcc ccatggccag acgctgttga tcaccggcgc gccggcttgt tcgcgggctt
     2641 cgttcaccgg gtgattgaac agcagcattt gcagctcgtt gagcatatgc cgccaatagc
     2701 tggcgcgctc gccgcgcggc agatgaaaat caatattctg cgccgccgcc tcggccagcg
     2761 gggtgaactc ggccgcgctg gcggcgtcca ggcgcagata ccagcggttg ggggtgggcg
     2821 cgtaaaagca caggccgtcc tggccaaagt ggctgttcag cgtcgccacc agggcgtcgg
     2881 cttcggcctg gttcagcgca aacatgcgcg cgtcggccag caagagataa tcctgctcga
     2941 cgcgcaaatg caccgggtcg gcgcacagcc agtggccgtc gtcggcggcc aggccgtcgg
     3001 cgcgggccag cgcgcgcgcc accggcaaat cagccggcgc ggcaaacagc tcggccaggc
     3061 aggccgaggc gctttgcgcc tgcgcgcgcg cctggccacg gcccaacagc cagcccaggg
     3121 ccggcaaggg cagctcgcgg gtggcttcgc ggcgcaagtc cgggtcggcc cacagcaaat
     3181 cggggaccac caaggtcaga tgagacagcg gcatggcaag gctccgctca gaccagggtc
     3241 accttggcga acttgcgctt gcccacttgc agcaccaggg tggcgccagc ggccagttgc
     3301 agttttttgt cttcgatttt ttcgccgtcg gccttgacgc cgccgccgtc gatcatgcgc
     3361 atggcttccg aggtggacgc caccaggccg gcttctttca gcacgctgcc aatcgccagc
     3421 acgccgcctt cgccggtttg cacggtgact tccggcaggt cgtcggggat ggcgttttgc
     3481 ttgaagcgcg cttcaaagtc ggccagcgcc gcttcggcgg cggcttgcga atgaaaacgc
     3541 gccaccagct cttgcgccag cagcactttt acatcgcgcg ggttgcgccc ggcggcgatg
     3601 tcggccttga actgctcgat ctcggccagc gggcgcagcg acagcagctc gtaatagcgc
     3661 cacatcagcg tgtcggacac gctcatcacc ttgccaaaga tgtcattggc ggcttccgag
     3721 atgccgatgt agttgcccag cgatttggac attttattga cgccgtccag gccttccagc
     3781 agcggcatca tgatcacgca ttgcggcttt tgcccgtgct gttcttgcag catgcggccc
     3841 atcagcaggt tgaatttctg gtcggtgccg cccagctcga tatcggcctg catggccacc
     3901 gagtcgtagc cctgcatcag cgggtacatg aattcatgca gcgaaatcgg ctggttgttg
     3961 ccaaagcgct ttttgaagtc gtcgcgctcc agcatgcgcg ccaccgtggt gctggccgcc
     4021 agcttgagca tgccgcccgc gcccagctgg ttcagccagg tggagttgaa acagatttcg
     4081 gtcttggccg ggtcgagaat cttgtacacc tgttcctggt aggtcttggc gttgatcgcc
     4141 acctgttccg gggtcagcgg cgggcgggtg gtgtttttgc cggtcgggtc gccgatcatg
     4201 ccggtgaagt cgccaatcaa aaacatgatg tggtggccca tgtcctgcaa ctgacgcatc
     4261 ttggtcagca ccacggtgtg gcccaggtgc aggtcggggg cggtggggtc aaagccggcc
     4321 ttgatgcgca gcgggcggtt ttctttcagc ttggcaatca gctcggcttc gatcagcaat
     4381 tcgtcggcgc cacggcggat ggtttccagg gcggcggcca gttcggcgga caggggggcg
     4441 ggtgcggtca tcggtcagtc attccagcgc cgccgccggt gggcggtcgg ctcaagtcag
     4501 ggcgcgcaag gcgcaatcag gtcaatcggc aattatcgca aataaaaaaa cccgcggcca
     4561 gccatggcag cgggtttgac gggtgggcca gcagggcgga catacgccgc ccggtctggc
     4621 atcagccctt tttggccttc aggttggcgg cgatgcgcat gcgcagcgcg ttcagcttga
     4681 taaagccgcc agcgtcgcgc tggtcgtagg cgccggcatc gtcttcaaag gtggcgatgg
     4741 tcgggtcgaa cagcgagtcg gttttggaat cgcggccaac cacaatcaca ttgcccttgt
     4801 acagcttcag gcgcacccag ccgttgacgg tttgctgggt gtggtcgatc agcgcttgca
     4861 gggccaggcg ctccgggctc caccagtagc cgttgtagat catgctggcg tagcgcggca
     4921 tcagatcatc cttcaggtgg gccacttcgc ggtccagggt gatcgattcg atggcgcggt
     4981 gagccttcag gatgatggtc ccgcccgggg tttcgtagca gccgcgcgac ttcatgccga
     5041 cgtagcggtt ttccaccagg tccagacggc cgatgccgtg cttgccgccc agctcgttca
     5101 gcttggtcag cacttgcgcc gggctcatgg ccacgccgtt cagcgccacg atgtcgccct
     5161 tggcgaattc cacgtccagg tattcggcgg cgtccggggc ttgttccggc gagacggtcc
     5221 agcgccacat ggattcttcg gcttcggcgg ccgggtcttc cagatggcgg ccttcaaacg
     5281 agatgtgcag caggttggcg tccatcgagt acggcgagcc gccgttgcgg tgcttcatgt
     5341 cgatggggat gccgttggtt tcggcgtagg ccagcagttt ttcgcgcgac agcagatccc
     5401 actcgcgcca cggggcaatc accttcacgc ccggcttgag cgcgtaggcg cccagctcga
     5461 aacgcacctg gtcgttgccc ttgccggtgg cgccgtgcga gatggcgtcg gcgccggtct
     5521 ggttggcgat ctcgatcagg cgcttggcga tcagcgggcg ggcaatcgag gtgcccagca
     5581 ggtattcgcc ttcgtacacg gtgttggcgc ggaacatcgg gaagacgaaa tcacgcacga
     5641 actcttcgcg caggtcgtcg ataaagatgt tttccggctt gatgccgaat tgcagcgcct
     5701 tggcgcgcgc cggctccagc tcttcgccct ggcccaggtc ggcggtgaag gtgaccactt
     5761 cacattgata ggtgtcttgc aaccacttca ggatcaccga agtgtccagg ccgccggagt
     5821 aggcgagtac gacttttttg acgtcagaca tgatcagttc tttcactcag acttataaag
     5881 caaccgcccg cgtcgggcgg cctttagaaa acggttattt cttgtcggcc acgcggccca
     5941 gcagcaggaa ttccaccagc gccttttgca catgcatgcg gttttcggct tcgtcccaca
     6001 ctacgctatt ggggccgtcg atcacctcgg cgtccacttc ttcgccgcga tgcgccggca
     6061 ggcagtgcat gaacatcgca tccggcttgg cggcggccat caccgcagcg gtgacgccat
     6121 agccggcaaa cgcccggtgg cgcgcctcgg cttcggcctc aaagcccatg ctggtgtaga
     6181 catccgtggt gacaatatcg gcgccgcgcg cggcgtccag cgggttgtcg cacacttcgt
     6241 agacgtccgg gccgtaatcc tgcgcgtcca gcaccgtcag ctcatagccc ttgggcgagg
     6301 cgatgcggaa cttgaaaccc aagagtttag ccgcttgcag ccaggtgcgg gcaacattgt
     6361 tgccgtcgcc aatccaggcc acggtgcgcc cgctgatcgg gccgcgctgc tccacccagg
     6421 tgaacagatc ggccagcacc tggcaggggt ggtattcgtt ggtcaggccg ttgatcaccg
     6481 gcgcgcggga aaacgccgcc aggcgctcaa ccagcgcttg ttcgaaggtg cggatcatga
     6541 tgatgtcgga catgcgcgac agcacgcgcg cagtgtcttc aatcggctcg ccccggccca
     6601 gctgcgagct tttggaatcc agaaacatgg catggccgcc catttgcgcc atgccggctt
     6661 caaacgaggc gcgggtgcgg gtggagttct tctcgaacac catcgacatg accttgccga
     6721 ccagcgggcg gtacagctcg ccatcgtagt gacgtcgctt caggattcgg ctgcgttcga
     6781 acaggtgcag gtattcgtca cgggtcaaat cgctgaattg caggtaatgt ctggctggct
     6841 tcatgatgaa aaaaggtccg tagggaaggc cgccggcaac gggcgggcca gacgcgcgcc
     6901 gcgcggccag cctgcacgcc catgcgtggg cgccaaaata tcacgcccgg ccaggccggg
     6961 cgcgtggggt tgggcagcac aagctgcaag tggtagttgt aacggattta gccttggctg
     7021 gcaaggcttg acgatgcccg tggcgtgaaa gcccgccttg ccggg
//
LOCUS       DAOPYX010000150         7056 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_12983_length_7056_cov_12.780174, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000150 DAOPYX010000000
VERSION     DAOPYX010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 7056)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7056)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7056
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12983_length_7056_cov_12.780174"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1734
                     /gene="msbA"
                     /locus_tag="PKN55_08645"
     CDS             <1..1734
                     /gene="msbA"
                     /locus_tag="PKN55_08645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014088522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005319 - lipid transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006869 - lipid transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A export permease/ATP-binding protein
                     MsbA"
                     /protein_id="HNX71352.1"
                     /translation="FGLYKRLFGYLKQYWRLFALSLLALAVAAATEPAFARLMKPLVD
                     GGFVERDPERMIWTPVAIVGLFLLRGLTSFINDYSTTWLAGHLVMRLREEMFSKLLRL
                     PVSYYDDNASGRVLSRIAYDVNQVTEAGFNIITVTVKDGITILGLLALLFYTDWQLTL
                     ICLTMIPVVGISIRYVGRRLRALSRQNQATMGQMTQVLGESIDCQRVVKVYGGAPYEL
                     RRFMAAANQLRQNGVKQAATSSSNTGITQLIVSVALAVIIYFASLRASHNQFTAGDFM
                     SFLTAMIMLFAPVKRITSINQSLQKGLAAAESVFGFLDEASEHDPGTRELKQPRGKLD
                     FNQVGFCYPKAEREALSAIDLHIAPGETVALVGSSGSGKTTLVNLLPRFYDPSAGQIL
                     LDDVPLAELRLANLRSHIAMVSQDVTLFNDSVAANIAYGCFEQVSREQIIEAARAANA
                     LDFIEAMPQGFDTLIGENGVRLSGGQRQRLAIARALLKNAPVLILDEATSALDTQSER
                     LVQAALERLMENRTTIVIAHRLSTIENADRIVVMQQGRIVEVGAHAELLALDGVYATL
                     HRLQFKEPQHG"
     gene            1727..2824
                     /locus_tag="PKN55_08650"
     CDS             1727..2824
                     /locus_tag="PKN55_08650"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014088521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNX71353.1"
                     /translation="MAELVVLHTESSKGWGGQEHRTLKEARGLARHGVRVLFACQPGS
                     RLAERASAEGFAVSLMRMRSSVDVGAVAALVKLIQRERVDVVNTHSGRDSLLAGLAGR
                     LAWRQPLVVRTRHLALPITSRFTYSVLPHHVVAVSEYVREYLISEGVAADQVSTIYTG
                     IEPDALALDGPSSLRGELGLGPDAPLVGTVAILRQKKGHHLIVDAVPEILRAVPDAHF
                     VFAGDGPQTDNLKAQIARLGVAGRVHLLGLRRDVANVLAGLDLFVLPTREEALGTAYI
                     EAMAMGLPIVGTQVGGVPEVVGDGDNGLLVPVDDSPALAEAIICLLKDPAMRAAMAAR
                     SRDRVASHFLTDTMCEHMLLLYRRLLAGRSR"
     gene            2827..3651
                     /locus_tag="PKN55_08655"
     CDS             2827..3651
                     /locus_tag="PKN55_08655"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014088520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HNX71354.1"
                     /translation="MPASVVPVLMYHHVSPTPGLITVSPAHFAAQMRWLKRQGYTTLK
                     LDALAGFLAGEPVPAKSVVVSFDDGYLDNWVYAHPVLEQYGLHAVLFLITRWLGDGPT
                     RPYAGQRQGALPACLSHHAGKTAIDAGQTDLVMMRWGEVLAARDAGTFEFHSHTHTHN
                     RWDKIYPGDADNKSKALAHDLASSRNLLTERLGEVSSHLCWPQGYYDDDYIRVAEAAG
                     FRHLYTTEPGVVGADSMASRLPRIVVKDRGLGWFAPRMWAYRQPALARWYLGRKSR"
     gene            3651..4784
                     /locus_tag="PKN55_08660"
     CDS             3651..4784
                     /locus_tag="PKN55_08660"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018748349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HNX71355.1"
                     /translation="MGTHLREHALKLAMRLAKRLDRRQPDPAELGSATIQRILVVSCT
                     AIGDTLMSTPAIRSLRLAYPHAHLTLLIHPAYRELFASLPGVNAMLGYRGGWKGFVRL
                     ALTLHRQSFDLAVILHGNEPQATPLAYLSGARWIFKLPNAGNPFQFLLSNREPQLRWE
                     DFQHGIEQRLATAALAGGAPTDWAMDMPVGDHERLLVDDWLARQGVKPAQRVLGLQAG
                     ASTVSRRWPAGRLAELARRLLAADPQLAIVLTGSAAELPLIERIAADIANPRAVISAG
                     AVPLAHLPALMARMSALVTPDTGALHLAVAVGTPVIALFAVSDWRRSGPAVALEKHTV
                     IQKWRTCEPCLSKRCPYPEPPCMDLISVDEVEAACRQRLDLPT"
     gene            4781..5974
                     /locus_tag="PKN55_08665"
     CDS             4781..5974
                     /locus_tag="PKN55_08665"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014088518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNX71356.1"
                     /translation="MSQRLLLLDNGREWGGGTNSLLALLARLDRARHAVDAVFYHDYA
                     GGPLGQLSRSLAALQVPLAIQPQRRQPLWAKLGKELARGVLTPLPGWRAAAVEQIELA
                     WRVRPNAQQLAARLRQSGYQALYMNNQPQSNLEGYLAAQLAGVPVIQHCRSNPALSPR
                     AVAAANQAARIIAVSHGIADSLRAQGVEPARIRVVPNGIDPTQPLPDPAACRARLGVS
                     ADTVVIGTVCSLLPRKGVADLLTAVARLQSATPLCVLVIGDGPQRAALEQQTHAAGLG
                     ECVRFVGFQAQVLEWTAALDICVLASASEGLPRVLLEAMLLGKPVVASRVTGSAELVV
                     DGHTGLLFPYADAEALAAALAALIADPARRQAMGAAGRARVLAEYTLDSYVAGVTRVF
                     DEVLS"
     gene            5971..>7056
                     /locus_tag="PKN55_08670"
     CDS             5971..>7056
                     /locus_tag="PKN55_08670"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HNX71357.1"
                     /translation="MMALLLTWLCWPWLAWRARAARATPVRRILLIQTAKIGDMLCAT
                     PVIAALRAAYPHAELTVLHDPITSRIIAHQPGLRRLAQPARAFRGLAGRRALLRLLRD
                     GQYDTVLTLSPNLSFWLAPFWAGAARRWSIVPTWPGRTLAAARACLSDGEAHQPGELV
                     LDTLARLLARHGVTLGRDKQIHLADSAAAALAALHLPAGRAIGLGVSAANKLKELGVP
                     KLVEVCQRLLAASDAHLVLIGSGGDSPLAQAIASQVNSPRLIDATGRFQLDELPALLQ
                     RLDAYVGVDSGITYLADTVHTPIVTVVGPTDPREQRALGQRVIRIAPVDLPCYPCAFV
                     FATPSRCHTGTRACIESISAQQIADAAL"
BASE COUNT         1064 a         2361 c         2379 g         1252 t
ORIGIN      
        1 tttggcctgt acaagcgtct gtttggctat ctcaagcaat actggcggtt atttgccctg
       61 tcgctgctgg cgctggccgt ggcggccgcc accgagccgg catttgcccg actgatgaag
      121 cccttggtgg atggcggctt tgtcgagcgc gaccccgagc gcatgatctg gacccccgtt
      181 gccatcgtcg gcctgttctt gctgcgcggc ctcaccagct tcatcaatga ttactccacc
      241 acctggctgg ccgggcatct ggtgatgcgg ctgcgcgaag agatgttcag caagctgttg
      301 cgcctgccgg tcagctacta cgacgacaac gcctcgggcc gtgtgctgtc gcgcatcgcc
      361 tacgacgtca atcaagtcac cgaagccggc tttaacatca tcaccgtgac ggtgaaggat
      421 ggcatcacca tcctcggcct gctggccctg ctgttttaca ccgactggca gctgaccttg
      481 atctgcctga ccatgattcc ggtggtgggc atcagcatcc gctacgttgg tcgccgcctg
      541 cgcgcgctgt cgcggcaaaa ccaggccacc atggggcaga tgacccaggt gctgggcgaa
      601 tccatcgact gtcagcgcgt ggtcaaggtg tatggcggcg cgccctacga gctgcgccgc
      661 tttatggccg ccgccaatca gctgcgccag aacggcgtca agcaggcggc gacctcgtcg
      721 tccaacaccg gcatcaccca gctgattgtc tcggtggcgc tggcggtgat catctacttt
      781 gccagcctgc gcgccagtca caaccagttc accgctggcg atttcatgtc gtttctgacg
      841 gcgatgatca tgctgtttgc cccggtcaag cgcatcacca gcattaatca atcgctgcaa
      901 aaaggcctgg ccgccgccga gagcgtgttt ggctttctgg acgaagccag cgagcacgac
      961 cccggcacgc gcgagctgaa gcagccgcgc ggcaagctgg actttaatca agtgggcttt
     1021 tgctacccca aggccgagcg cgaggcgctg tcggcgattg atctgcacat tgcccccggc
     1081 gaaaccgtgg ccctggtggg cagttcgggc agcggcaaaa ccacgctggt gaacctcttg
     1141 ccgcgctttt atgaccccag cgccgggcaa atcctgctgg acgacgtgcc gctggccgag
     1201 ctgcgcctgg ccaatctgcg cagccacatc gccatggtca gccaggatgt gacgctgttc
     1261 aacgacagcg tggccgccaa tatcgcctat ggctgctttg aacaggtcag ccgcgagcaa
     1321 atcatcgagg cggcgcgcgc cgccaatgcg ctggacttta tcgaggccat gccgcagggc
     1381 tttgacacgc tgattggtga aaacggcgtg cgcctgtccg gcggccagcg ccagcgactg
     1441 gcgattgccc gcgcgctgct gaaaaacgcg ccggtgctga ttctggacga agccaccagc
     1501 gcgctggaca cccagtccga acggctggtg caagcggcgc tggagcggct gatggagaac
     1561 cgcaccacga ttgtgattgc gcatcggctg tccaccattg aaaatgctga tcgcatcgtg
     1621 gtgatgcaac agggccgcat tgttgaagtg ggcgcccacg ccgagctgtt ggcgctggat
     1681 ggcgtctacg ccaccctgca ccgtctgcaa ttcaaggagc cgcaacatgg ctgagctggt
     1741 ggtgctgcac accgagtcgt ccaaaggctg gggcggccag gagcaccgca cactgaaaga
     1801 agcgcgcggc ctggcccgcc atggcgtgcg cgtgctgttt gcctgccagc ccggcagccg
     1861 gctggccgag cgcgccagcg ccgagggctt tgccgtcagc ctgatgcgca tgcgcagcag
     1921 cgtggatgtt ggcgcggtgg cggccttggt caaactgatt cagcgcgagc gggtggacgt
     1981 ggtcaacacc cacagcggcc gcgacagcct gctggccggc ctggccggcc gcctggcctg
     2041 gcgccagccg ctggtggtgc gcacccgcca tctggccctg ccgatcacct cgcgctttac
     2101 ctattcggtg ttgccccacc atgtggtggc ggtcagcgaa tatgtgcgcg aatacctgat
     2161 cagcgaaggc gtggcggcgg atcaggtcag caccatttac accggcatcg agccggatgc
     2221 gctggcgctg gatggcccgt cctcgctgcg cggcgagctg ggcctggggc cggatgcgcc
     2281 gctggtgggc acggtggcga ttctgcgcca gaaaaaaggc catcacctga ttgtggacgc
     2341 cgtgccggag attttgcgcg ccgtgccgga cgcgcatttt gtgtttgccg gcgatggccc
     2401 gcagaccgac aatctcaagg cgcaaatcgc ccgcctgggc gtggccgggc gcgtacacct
     2461 gctgggcctg cgccgcgatg tggccaatgt gctggccggg ctggatttgt ttgtgttgcc
     2521 cacccgcgaa gaagcgctgg gcaccgccta tatcgaagcc atggccatgg gcctgccgat
     2581 tgtcggcacc caggtgggcg gcgtgccgga agtggtgggc gatggcgaca acggcctgct
     2641 ggtgccggtg gacgacagcc cggccttggc cgaggcgatt atctgcctgc tcaaggaccc
     2701 ggccatgcgc gcggccatgg cagcgcgcag ccgtgaccgg gtggccagcc acttcctgac
     2761 tgacaccatg tgcgaacata tgctgttgct gtatcgccgc ctgttggcgg gccggagccg
     2821 ataaccatgc ctgcatccgt cgttcccgtg ctgatgtacc accatgtcag ccccacgcct
     2881 ggcttgatca ccgtgtcgcc cgcgcacttt gccgcgcaga tgcgctggct caagcgtcag
     2941 ggctacacca cgctgaagct ggatgcgctg gcgggttttc tggcgggcga gccggtgccg
     3001 gctaaatctg tggtggtcag ctttgacgat ggttatttgg acaactgggt atacgcgcat
     3061 ccggtgctgg agcaatacgg cctgcatgcc gtactgtttt tgattacccg ctggctgggc
     3121 gatggcccga cgcggcctta tgccggccag cgtcagggcg ctttgcccgc ctgcctgagc
     3181 caccatgccg gcaagacggc catcgacgct ggccagaccg atttggtgat gatgcgctgg
     3241 ggcgaagtgc tggctgcccg cgatgccggc acgtttgaat ttcacagcca tacccacacc
     3301 cataaccgct gggacaaaat ctaccccggc gacgccgaca acaagtccaa ggcgctggcg
     3361 catgatctgg ccagcagccg caacctgttg accgaacgcc tgggtgaagt cagtagccac
     3421 ctgtgctggc cgcaaggcta ttacgacgac gactatatcc gcgtggctga agccgcaggt
     3481 tttcgtcatc tgtacactac cgagccaggc gtggtgggcg ctgacagcat ggccagccgg
     3541 ctgccgcgca tcgtggtgaa agatcgtggg ctgggctggt ttgccccacg catgtgggcc
     3601 taccggcagc cggcgttggc gcgctggtat ctggggcgga agtcgcgctg atgggcacgc
     3661 acctgcgcga gcacgcgctg aaactggcca tgcgcctggc caagcggctg gatcgtcgcc
     3721 agcccgatcc ggccgagctg ggcagcgcca ccatccagcg cattctggtg gtgtcctgca
     3781 ccgccatcgg cgacacgctg atgtccaccc cggccatccg cagcctgcgg ctggcgtatc
     3841 cgcatgccca cctgaccttg ctgattcacc ccgcctaccg tgagctgttt gcctcgctgc
     3901 ccggcgtgaa cgccatgctg ggctatcgcg gtggctggaa gggttttgtg cgactggcgc
     3961 tgaccctgca tcgccaatca tttgacctag cggtgattct gcatggcaac gaaccgcaag
     4021 ccacgccgct ggcgtatttg tctggcgcgc gctggatttt caagctgccc aacgccggca
     4081 acccttttca gtttttattg agcaaccgcg aaccacagct gcgctgggag gatttccagc
     4141 acggtatcga gcaaagactg gccactgccg ccttggccgg tggcgcaccg actgactggg
     4201 cgatggatat gccagtgggc gaccacgagc ggctgttggt ggatgactgg ctggcgcgcc
     4261 agggggtaaa gccggcccag cgcgtgctgg gcctgcaagc cggcgccagc acggtcagcc
     4321 ggcgctggcc ggccgggcgg ctggccgagc tggcgcgccg gctgctggcc gccgacccgc
     4381 aactggcgat tgtgctgacc ggctcggctg ccgagctgcc gctgattgag cgcatcgccg
     4441 ccgacatcgc caacccgcgc gccgtcatca gcgccggcgc cgtgcccttg gcgcatttgc
     4501 cggcgctgat ggcgcgcatg agcgcgctgg tcacccccga caccggcgcc ttgcacctgg
     4561 cggtggcggt gggcacgccg gtgattgccc tgtttgccgt gtccgactgg cggcgcagcg
     4621 gcccggcggt ggcgctggaa aaacacacgg tgattcaaaa gtggcgcacc tgtgagccct
     4681 gtctgtccaa gcgctgcccc tacccggaac cgccctgtat ggacttgatc agcgtggacg
     4741 aagtcgaagc cgcctgtcgc cagcgcctgg atctacccac atgagtcagc gcctgttatt
     4801 gctggacaat ggccgtgaat ggggcggcgg caccaacagc ctgctggcct tgctggcgcg
     4861 gctggatcgc gcccgccatg cggtggacgc ggtgttttat cacgattatg ccggcggccc
     4921 gctgggccag ctcagccgca gcctggcggc gctacaggtg ccgctggcca ttcagccgca
     4981 gcgccggcag ccgctgtggg ccaagctggg caaagagctg gcgcgcggcg tgctgacccc
     5041 gctgcccggc tggcgcgccg ccgccgtcga gcagatcgaa ctggcctggc gggtgcgccc
     5101 caacgcccaa cagctggccg cgcggctgcg ccagagcggc tatcaggcgc tgtatatgaa
     5161 caaccagccg caatccaatc tggaaggcta tctggccgcc caactggccg gcgtgccggt
     5221 gattcagcac tgccgcagca atccggcgct gtcgccgcgc gcggtggccg ccgccaacca
     5281 ggcggcgcgc atcatcgccg tgtcgcacgg cattgccgac agcctgcgcg cgcagggggt
     5341 agagccggcg cgcatccgtg tggtgcccaa cggcatcgac ccgacccagc ccctgcccga
     5401 ccctgccgcc tgccgcgccc ggctgggcgt gtcggccgac accgtggtga ttggcacggt
     5461 gtgctcgctg ctgccgcgca agggcgtggc ggatttactc actgccgtgg cgcggctgca
     5521 gagcgccacg ccgctgtgtg tgctggtgat tggcgacggc ccgcagcgcg cggcgctgga
     5581 gcaacagacc cacgccgccg ggctgggcga gtgcgtgcgt tttgtcgggt ttcaggcgca
     5641 ggtgctggaa tggaccgccg cgctggatat ctgcgtgctg gccagcgcca gcgaaggcct
     5701 gccgcgcgtg ctgctggaag ccatgttgct gggcaagccg gtggtggcct cgcgcgtcac
     5761 cggctcggcc gagctggtgg tggatggcca caccggcctg ttgtttccct acgccgacgc
     5821 cgaggcgctg gccgccgcgc tggccgcctt gatcgccgac ccggcccgcc gccaggccat
     5881 gggcgccgcc gggcgggcgc gggtgttggc cgaatatacg ctggacagct atgtggccgg
     5941 cgtcacgcgc gtgtttgacg aggtgttgtc atgatggctt tgttgctgac ctggctgtgc
     6001 tggccatggc tggcctggcg cgcccgcgcc gcgcgcgcca ccccggtgcg ccgcatcctg
     6061 ctgattcaaa ccgccaagat tggcgacatg ctgtgcgcca cgccagtgat tgccgcgctg
     6121 cgcgccgcct atccgcacgc cgagctgacc gtgctgcacg accccatcac cagccgcatc
     6181 atcgcccacc agccgggcct gcgccgtctg gcgcagccgg cgcgggcgtt tcgcggcctg
     6241 gcgggccgcc gcgcgctgct gcgcctgctg cgcgatggcc agtacgacac cgtgctgacc
     6301 ttgtcgccca atttgtcgtt ctggctggcg ccgttctggg ctggcgccgc gcggcgctgg
     6361 tcgattgtgc ccacctggcc ggggcgcacg ctggcggcgg cgcgcgcctg cttgagcgat
     6421 ggcgaggcgc accagccggg cgaattggtg ctggacaccc tggcccgcct gctggcccgc
     6481 cacggcgtga cgctgggccg cgacaagcag attcatctgg ccgacagcgc cgccgccgcg
     6541 ctggcggcgc tgcacctgcc ggccgggcgc gccatcggcc tgggggtgag cgccgccaac
     6601 aaactcaaag aactgggcgt gcccaagctg gtggaagtct gccagcgcct gctggccgcc
     6661 agcgacgccc atctggtgct gattggcagc ggcggcgaca gcccgctggc gcaggcgatt
     6721 gccagccagg tcaacagccc gcgcctgatc gacgccaccg ggcgctttca gctggacgag
     6781 ctgccggcgc tgttgcagcg gctggatgct tatgtcggcg tggattccgg catcacgtat
     6841 ctggccgaca ccgtgcacac gccgattgtc accgtggtcg gccccaccga cccgcgcgag
     6901 cagcgcgcgc tggggcagcg ggtgattcgc atcgcgccag tggatttgcc gtgttatccc
     6961 tgcgcctttg tgtttgccac gccctcgcgc tgccacaccg gcacgcgcgc ctgtattgaa
     7021 tcgatttccg cccagcagat tgccgacgcc gcgctg
//
LOCUS       DAOPYX010000151         7000 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_13137_length_7000_cov_8.522822, whole genome shotgun sequence.
ACCESSION   DAOPYX010000151 DAOPYX010000000
VERSION     DAOPYX010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 7000)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7000)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7000
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13137_length_7000_cov_8.522822"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(73..231)
                     /locus_tag="PKN55_08675"
     CDS             complement(73..231)
                     /locus_tag="PKN55_08675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX71358.1"
                     /translation="MKLHLADIALTRARLFEDKAELAKARALIEECGYGRRLPELEDA
                     ERRLGLAR"
     gene            complement(182..2938)
                     /locus_tag="PKN55_08680"
     CDS             complement(182..2938)
                     /locus_tag="PKN55_08680"
                     /inference="COORDINATES: protein motif:HMM:NF025056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HNX71359.1"
                     /translation="MRQIFISYSHQDEAWKDHLLSHLNVLAGEGLEVWDDRRIAAGDD
                     WLPEIERAIAACDVAVLLISRHFLTSKFIAGQEVPPLLQRRQAQGVRVIPLIISPCQW
                     QRIDWLKAIQGRPRDGKPLSGMSQHDADLVLSNLAGEIADLAPSAAIPTQPTAEMPPR
                     IGFFHFPAGAEHFFGREEELAVLDAAWADSQHSHVVEWVAPGGVGKSSLVKRWLENVQ
                     RDGWRGARRVYVWSFYSQGASHDRQASDDDFLSAALAWFGVKLDPATHPWDKGQRLAE
                     AVAASRTLLVLDGVEPLQYPPGPLAGELRAPGLKALLECLASVGQPGLCLVTSRAQLT
                     DLAQYQRNASRPAGAVLRRQLGNLGELDGARLLHALGANKAGSVAVNNDDEELKAASR
                     EVRGHALTLSLLGRYLALAYAGDIRQRAQVDFQEADDELDDGRAFKVIAAYEHWFISH
                     EKNGERELAALRLLGFFDRPASLKSLRALRQPPALPEVSLPLVNLKPTQWQATLQRLV
                     DCGLAFYNEDNTALDAHPLVREYQAKVLQTRFRPAWRQGNKLLYKLLADSAKHWPDDL
                     EGLQPLYQAVVHGCRAGLYQQALDEVYHERILRGTEPNGAYSIHKLGAFGADLGAVAC
                     FFAEPWQRLAPGLSVGDQAWLLSVAAFSLRALGRLAEGLEPMRAGAEMRVEQADWKNA
                     ASSYGNLAELQLSLGRIALAVADAERSVDYADRSGDAAWRMALRTTLADARHQQGKQE
                     AARRGFAEAEAMQAERQPQHPLLYSQGGFRYGELLLAEAEQAAWGGPATAEGSEACAQ
                     VAQRAAQTLAWAEINQASLLSFPRPRPLDPRPLRALRRPAARSPARRGRPAAHRSRCR
                     RPAPGRHYGPSPPWPPHPRLAAPLPERSPRRASRSRRSPAHRRARRHEAPSRRHRPDP
                     RPAV"
     gene            complement(3264..5492)
                     /locus_tag="PKN55_08685"
     CDS             complement(3264..5492)
                     /locus_tag="PKN55_08685"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNX71360.1"
                     /translation="MGCRIRPLTAAVALAFVSSSGWQLAQAQALDEVVVKASKQATLP
                     GASQAGELAAQSAASRDTASLLKNIPGVSLYGAGAVSSLPAIHGLADDRLRIKVDGMD
                     LIASCPNHMNPALSYLDPSQVGTLTVYTGISPVSLGGDSIGGSIVANTRAPEFAEPGQ
                     GRLLKGEVGAFFRSNNNARGGNASVTYATENVSLSYSGAYSEADNYKAGKDFKTFTAS
                     GRPGHDLARDEVGSSAYRSANHTLDLAVKHDNHLFEAKLGYQNMPEQLYPNQRMDLLN
                     NEQKRINLRWLGEFAWGKLETRAYHETVKHYMDFGADKQFQYSTAPGMPMHTEGKTTG
                     ATVKAEIDLAARSLLRLGGEYQHYTLNDWWPASGTGGMSPYTFININDGQRNRLALFG
                     EWEKQLTPQWTTQLGVRYERVNTSTGQVHGYNQNNNPTSAGMSMMNQTNDAARFNTSA
                     KRDSTDNNWDFTALAHYKIDATQDIEFGLARKVRSPNLYERYSWSTANMMAIMNNFVG
                     DGNGYIGNPDLKPEKAHTLSATFDWHDAERRRELKLTPHYSHVTDYIDAIQWDNVSNT
                     ARTTPLRNQFTTLRYANQTARLYGVDLSGQLPLANTAWGEFGLKGLLSYVRGKNTDTG
                     DDLYNIMPLNAKLTLTHKIGGWDNALEIEGVSGKKKVADARNEIQTGGYALVNLRASY
                     SWRQARLDFGVANLFDRGYALPTGGAYTGQGTTMGINSIPWGIAVPGMGRSLYAGVNV
                     KF"
     gene            complement(5596..5757)
                     /locus_tag="PKN55_08690"
     CDS             complement(5596..5757)
                     /locus_tag="PKN55_08690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX71361.1"
                     /translation="MTRTHDTHHFATHDDVALFYRRWPAQGPRRGAVLLFHRGHEHGA
                     RMAHLVVPS"
     gene            complement(5754..6365)
                     /locus_tag="PKN55_08695"
     CDS             complement(5754..6365)
                     /locus_tag="PKN55_08695"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002641976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HNX71362.1"
                     /translation="MTLYQLKPRFQALLRPGVAWLAAHGVTANAVTLAAMLLSLALGA
                     GLCLAGPQSPQWFLLLPGWMLARMALNAVDGMLAREFGQQSALGAYLNELADVLADAA
                     LFAPFALQPALGWPAVCAVIVLSGVSEMAGALGPMVGAPRQYDGPLGKSDRALLFGAL
                     GLWLGLAGSLPAAAFWLMPVAILALVVNMINRIRSGLRAIQTP"
     gene            complement(6542..>7000)
                     /locus_tag="PKN55_08700"
     CDS             complement(6542..>7000)
                     /locus_tag="PKN55_08700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010931119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-Mn family superoxide dismutase"
                     /protein_id="HNX71363.1"
                     /translation="NLIKGTEFENASLEEIVKKSSGGIFNNAAQVWNHTFYWFGLSPN
                     GGGEPTGALAEAINAKWGSFDEFKKAFNAVAAGTFGSGWAWLVKAADGSLDLVFTSNA
                     ATPLTTDKTPLLTCDVWEHAYYIDYRNSRPNYLEGFWKLVNWDVVAKHFA"
BASE COUNT         1173 a         2235 c         2301 g         1291 t
ORIGIN      
        1 aaatagacga tcaaacccgg catcgcccgc cgggggcggg tggtcagcgg cggggtgggc
       61 aatcttcccg cgtcaccggg ccagccccag tcgccgctct gcatcctcca gctccggcaa
      121 gcgtcgtccg tagccgcatt cttcaatcag cgcacgggcc ttggccagtt cagctttgtc
      181 ctcaaacagc cgggcgcggg tcagggcgat gtcggcgaga tggagcttca tgccgccgcg
      241 ctcggcgatg cgctgggctt cggcgagatc ggcttgcgcg ccggggagat cgttcaggca
      301 gtggcgcagc caggcgcggg tgaggaggcc acgggggaga tggtccatag tgcctgcctg
      361 gcgcaggccg gcgacagcgg cttcggtgtg ctgctgggcg tcctcgccgg gcgggcgacc
      421 gtgcagcagg tcggcgtaga gcgcgcaacg ggcgagggtc aagtggtcga gggcgaggga
      481 aagaaaggag ggacgcttga ttgatttcgg cccatgctaa ggtctgagcc gcccgctgcg
      541 ccacctgggc gcacgcttca ctgccctctg ccgttgccgg cccgccccag gcggcctgct
      601 cggcctcggc cagcagcagc tcgccatacc gaaagcctcc ctgcgagtag agcagcgggt
      661 gctggggttg gcgctcggcc tgcatggcct cggcctcggc aaaaccgcga cgagcggctt
      721 cctgcttgcc ttgctggtgg cgggcatcgg ccagggtggt gcgcaatgcc atgcgccaag
      781 cggcatcgcc actgcggtcg gcgtagtcca ctgagcgctc ggcgtcagcc acggccagcg
      841 cgatacggcc caggctcagt tgcagttcgg caagattgcc gtagctggag gctgcgtttt
      901 tccagtccgc ttgctccacc cgcatctccg ccccagcccg catcggttcc aacccctccg
      961 ccagccggcc cagggcgcgc agactgaagg ccgctacgct gagcagccag gcttggtcgc
     1021 ctaccgacaa gcctggcgcc agccgctgcc agggttctgc gaagaaacag gccaccgccc
     1081 ccaggtcggc gccgaaggcg ccgagcttat ggatgctata ggcaccgttc ggctccgtgc
     1141 cacgcaaaat gcgttcgtga tacacctcat caagcgcctg ctggtacaac cccgcccgac
     1201 aaccatgcac caccgcctga tagagcggtt gcaagccttc cagatcatct ggccaatgct
     1261 tggcgctgtc ggccaaaagc ttataaagaa gcttgttccc ttgccgccag gccggacgga
     1321 agcgcgtctg caacaccttg gcctggtatt cccgcaccag cgggtgcgca tccagcgcgg
     1381 tgttgtcctc gttgtaaaaa gccagcccgc aatcgaccag cctttgcaag gtggcttgcc
     1441 attgcgtcgg tttcaggttc accaagggca ggctgacctc gggcagggct ggtggttggc
     1501 gcagggcgcg caggcttttc aggctggccg ggcggtcgaa aaagcccagc aggcgcaagg
     1561 cggccagttc ccgttcgccg tttttctcgt gactgataaa ccagtgctcg taggccgcga
     1621 taaccttaaa agcacggccg tcatccagct cgtcatcggc ctcctggaaa tccacctgcg
     1681 cccgctggcg gatgtctccc gcgtaagcca gcgccaggta gcggcccagc aggctcagcg
     1741 tcagggcatg gccgcgcact tcgcggctgg cggctttcag ttcctcgtcg tcgttgttca
     1801 ccgcaacgct gccggctttg ttggcgccaa gcgcatgcag cagacgggcg ccgtcaagct
     1861 cgccgagatt gcccagctgg cgtcgcagca ccgcccccgc cgggcggctg gcgttgcgct
     1921 ggtattgggc caagtccgtc agctgcgccc ggctggtgac caggcacagg ccgggctgac
     1981 ccacactggc caggcactcc agcagcgcct tgaggcccgg cgcgcgcaac tcgccggcca
     2041 gcgggccggg cggatattgc agcggctcga cgccgtccag caccaagagc gtgcgcgagg
     2101 cagccacggc ctcggccagg cgctggccct tgtcccaggg gtgggtggcc gggtccagct
     2161 tgacgccaaa ccaggccagg gcagcgctga gaaagtcgtc atccgacgcc tgccggtcat
     2221 ggctggcgcc ctggctgtaa aagctccaga cgtacacccg ccgcgccccg cgccaaccgt
     2281 cccgctgcac attctccagc cagcgcttga ccagcgaact cttgcccacg ccgccgggcg
     2341 cgacccactc caccacatgg ctgtgttggc tatcggccca ggcggcatcc agcaccgcca
     2401 gctcttcctc acggccgaaa aaatgctcag cgccggccgg gaaatgaaaa aaaccaatac
     2461 gcggcggcat ttctgctgtt ggctgcgtgg ggatagcggc agatggggcg agatcggcga
     2521 tctcgccggc caggttggaa agcacaaggt cagcatcgtg ctggctcatg cccgacaagg
     2581 gcttgccgtc gcgcggccgg ccctgtatcg ccttcagcca gtcgatgcgc tgccactggc
     2641 acgggctgat gatcaacggg atgacccgaa ccccctgcgc ctggcggcgt tgcagcaaag
     2701 gcggcacttc ctgacctgca atgaattttg aggtgaggaa gtggcgagaa atcagcagca
     2761 ccgccacatc acatgcggca atggctcgct caatctctgg caaccaatcg tctccagcgg
     2821 caatccggcg gtcatcccag acttccagcc cctcgccggc caatacgttc agatggctga
     2881 gcagatggtc tttccaagcc tcgtcttggt ggctatagct gatgaatatt tggcgcacag
     2941 catggattcc ggggtggttt ctcgatgccc gccccggcgt tcggcttcgc gcttgatcgc
     3001 atgagaaatc cgttcggggc ggatgatgcg aaattatgcc agaagtctgc gccacgggat
     3061 ggcggcgtgg cgcttgcgcg ctggccttgg atgagttggc ggccatggtg caagaagccg
     3121 gtatgggcgc tgagtgacac ggcacacgcc tgccgcccct cgccgccggc atgcgccctc
     3181 atccaatcgt ctcccccaaa ataacacccc agcagattcc tccgccgggg tgtcacccac
     3241 atccctttct acctgctgcc caatcagaac ttcacattca cccccgcata cagcgagcgg
     3301 cccatgccgg ggacggcgat gccccagggg atgctgttga tgcccatggt ggtgccctgg
     3361 ccggtgtagg cgccgccggt gggcagggcg tagccacggt caaacaggtt ggcgacgcca
     3421 aaatccagcc gtgcttgccg ccagctgtag ctggcgcgca ggttgaccag ggcgtagccg
     3481 ccggtctgga tttcgttgcg ggcgtcggcg acttttttct tgccgctcac gccttcgatt
     3541 tccagcgcgt tgtcccagcc gccgattttg tgggtgaggg tcagcttggc gttcagcggc
     3601 atgatgttgt acagatcgtc gccggtgtcg gtgtttttgc cgcgcacata gctgagcagg
     3661 cctttcaggc cgaattcgcc ccaggcggtg ttggccagcg gcagttggcc ggagaggtca
     3721 acgccgtaca ggcgggcggt ctggttggca tagcgcaggg tggtgaattg attgcgcagc
     3781 ggggtggtgc gggcggtgtt gctgacgttg tcccactgga tggcgtcgat gtagtcggtc
     3841 acatggctgt aatgcggggt gagcttgagc tcgcggcggc gctcggcgtc gtgccagtca
     3901 aaggtggcgg acagggtgtg ggctttttct ggcttgaggt cggggttgcc gatatagccg
     3961 ttaccgtcgc caacaaagtt gttcatgatc gccatcatat tggcggtgga ccaggaataa
     4021 cgctcgtaca gattgggcga gcgcaccttg cgcgccaggc caaattcgat gtcctgggtg
     4081 gcgtcgattt tgtaatgcgc cagggcggta aagtcccagt tattgtcggt gctgtcgcgt
     4141 ttggcactgg tgttgaagcg ggcggcgtcg ttggtctggt tcatcatcga catgccggcg
     4201 ctggtcgggt tgttgttctg gttgtagccg tgcacctgcc cggtactcgt gtttacccgc
     4261 tcgtaacgca cgcccagctg ggtggtccat tgcggggtga gttgtttttc ccattcacca
     4321 aacagcgcca ggcggttgcg ctggccgtcg ttgatattga tgaaagtgta cggcgacatg
     4381 ccgccggtgc cggaggccgg ccaccagtcg ttcagcgtgt agtgctggta ttcgccgccc
     4441 aggcgcagca ggctgcgcgc ggccaggtcg atttccgctt tgaccgtggc gccggtggtt
     4501 ttgccttcgg tgtgcatcgg catgccaggc gcggtgctgt actggaattg cttgtcagcg
     4561 ccaaagtcca tgtagtgctt gacggtttcg tgataggcgc gggtttccag cttgccccag
     4621 gcgaattcgc ccagccagcg cagattgatg cgcttttgct cgttgttgag caggtccatg
     4681 cgctggttgg ggtagagctg ttcgggcatg ttctggtagc ccagcttggc ttcaaacaga
     4741 tggttgtcgt gtttgaccgc caaatccagc gtgtggttgg cgctgcggta ggcgctggag
     4801 cccacttcgt cgcgcgccag gtcgtggccg gggcggccgc tggcggtgaa agttttgaaa
     4861 tccttgccgg ctttgtagtt gtcggcttcg ctataggcgc cgctgtagct caggctgacg
     4921 ttttcagtgg cgtaagtgac cgaggcattg ccgccacggg cgttgttgtt gctgcggaaa
     4981 aacgcgccga cttcgccctt gagcagccgg ccctggccgg gctcggcaaa ttccggcgcg
     5041 cgggtgttgg cgacaatgct gccgccaatg ctgtcgccgc ccaggctgac cgggctgatg
     5101 ccggtgtaca ccgtcagcgt gcccacttgg ctggggtcga ggtaggacag cgccgggttc
     5161 atgtggttgg ggcaggaggc aatcaggtcc atgccgtcca ccttgatgcg cagccggtcg
     5221 tcggccaggc cgtggatggc cggcaggctg gacaccgcgc cggcgccgta caggctgacg
     5281 ccggggatgt ttttcagcag cgaggcagtg tcgcggctgg cggcgctttg cgcggccagc
     5341 tcgccggcct ggctggcgcc gggcagcgtg gcctgtttgc tggccttgac caccacttca
     5401 tccagcgctt gcgcctgggc cagttgccag cccgacgacg acacaaaagc cagcgctacg
     5461 gcggcggtta acggacggat gcggcatccc atgaaacatt ccccgaactt tatcaataag
     5521 ggtgaattgt ccgggagtcg ggcaagcgcc ggaatgcggc aaattgtcgc actcctggat
     5581 cggggctggc tggattcagg aggggacaac cagatgggcc atgcgtgcgc catgctcgtg
     5641 gccacggtgg aacaacagca ccgcgccgcg tcgcggccct tgcgccggcc agcggcgata
     5701 aaacagcgcc acatcgtcat gcgtggcaaa gtgatgggta tcgtgagttc gggtcatggg
     5761 gtctggatgg cgcgcaggcc actgcgaatg cggttaatca tattgaccac cagcgccagg
     5821 atggccaccg gcatcagcca gaatgctgca gccggcaggc tgccggccag gcccagccac
     5881 aggcccagcg cgccaaacag caaggcgcgg tcgcttttgc ccaacgggcc atcatactgg
     5941 cgcggtgcgc ccaccatcgg ccccagtgcg ccggccatct cggatacccc ggacaataca
     6001 atcaccgcac acaccgccgg ccaacccagc gccggctgca aggcaaacgg cgcaaacaag
     6061 gccgcgtcgg ccaatacatc ggccagctcg ttcagatacg cccccagtgc tgactgctgg
     6121 ccaaactcgc gcgccagcat gccatccacc gcgttcagcg ccatgcgcgc cagcatccag
     6181 cccggcagca gcaaaaacca ctgcggcgac tgcggccccg ccaggcacaa gcccgccccc
     6241 agcgccagcg acagcaacat ggcggccagc gtgaccgcat tggccgtcac cccatgcgcc
     6301 gccagccagg ccaccccagg gcgcagcagc gcctgaaagc gcggcttgag ttggtaaagc
     6361 gtcagcattg atcactcatc gggcaaaaac cccattgtgc atatgcctga cgggtgtgtc
     6421 acctgcgcgg ccagcaaatt ggcatgccca gccgacaaaa caacgccgcc ccggccagaa
     6481 catatccgac cggggcggcg tgggcgtctc ggccagcctc aggctggccg tgtcaccgga
     6541 atcaggcgaa gtgcttggcc accacgtccc agttcaccag cttccagaag ccttccaggt
     6601 agttcgggcg gctgttgcgg tagtcgatgt agtaggcgtg ctcccacaca tcacaggtca
     6661 gcagcggggt cttgtcggtg gtcagcgggg tggcggcatt gctggtgaac accagatcca
     6721 gcgaaccatc ggccgccttc accagccagg cccagccaga accaaaggtg ccagcggcca
     6781 cggcgttgaa cgcctttttg aactcgtcaa agctgcccca cttggcgttg atcgcctcag
     6841 ccagcgcgcc agtcggctcg ccgccgccgt tcggggacag gccaaaccag tagaaggtgt
     6901 ggttccacac ctgggcggcg ttgttgaaga tgccgccgct ggatttcttg acgatttctt
     6961 ccaggctggc gttctcgaac tcggtgccct tgatcaggtt
//
LOCUS       DAOPYX010000152         6969 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_13208_length_6969_cov_13.432311, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000152 DAOPYX010000000
VERSION     DAOPYX010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 6969)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6969)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6969
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13208_length_6969_cov_13.432311"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1110
                     /gene="mmsA"
                     /locus_tag="PKN55_08705"
     CDS             <1..1110
                     /gene="mmsA"
                     /locus_tag="PKN55_08705"
                     /EC_number="1.2.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014087834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004491 - methylmalonate-semialdehyde
                     dehydrogenase (acylating) activity [Evidence IEA];
                     GO_process: GO:0006574 - valine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HNX71364.1"
                     /translation="ASGVDTYTLLQPLGVCAGITPFNFPAMIPLWMFPMAIATGNTFV
                     LKPSEQDPLVTMKLVKLALEAGVPPGVLNVIHGGEDAVNALCDHPDIKAISFVGSTRV
                     GTHVYNRASLNGKRVQCMMGAKNHAVVMPDANREQALNQLVGAAFGAAGQRCMATTVA
                     VLVGAAREWVPELVAKAQGLRVGAGKDNPDVGPMISCAARQRALNLVAQGLEQGAVLE
                     LDGRGVQVDGYPNGNFFGPTILSGVKPGMAVYDQEIFGPVLVLTEADSLDEAIELINS
                     NPNGNGTAIFTQNGAVARKFQEDIDVGQVGINVPIPVPVPLFSFTGSRGSKLGDLGPY
                     GKQVVMFYTQTKTITQRWFDDPASMGKVNTTISLK"
     assembly_gap    1127..1226
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1319..2479
                     /locus_tag="PKN55_08710"
     CDS             1319..2479
                     /locus_tag="PKN55_08710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008953969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNX71365.1"
                     /translation="MDFSLSDDQRAFQDAARAFARHELAPHAADWDAESIFPREVIAR
                     AGELGFCGLYTPETYGGLGLSRLDAAIVFEELAAGCTSTTAYITIHNMVSWMFATWAR
                     PEVAERWAPFLASGEKLGSYCLTEPNAGSDAANLRTRAERRGDRYVLNGSKMFISGAG
                     DTDLLLVMARTGEAGPKGISAFAVPADLPGIHYGKKEHKMGWNSQPTRAITFDNVEVP
                     DDHLLGEEGQGFSMAMKGLDGGRINIATCSVGTAQAALERAQAYLHERKQFGRELAEF
                     QALQFKLADMATELVAARQMVRLAAWKLDHGSADATTYCAMAKRFATDVGFKVANDAL
                     QLHGGYGYIREYPLERHVRDNRVHQILEGTNEIMRVIVARAILRPGGAEDIR"
     gene            2476..3258
                     /locus_tag="PKN55_08715"
     CDS             2476..3258
                     /locus_tag="PKN55_08715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008954513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HNX71366.1"
                     /translation="MTHYPHLLLDIQQHTALVTISNPPANTWNADSLNGLIELVHELN
                     ANRDIYALVLTGEGSKFFSAGADLNMFADGDPAVARKMARLFGQAFETLTDFRGVSIA
                     AINGYAMGGGLEVALACDLRIAETQAQMALPEATVGLLPCAGGTQNLPWLVGEGWAKR
                     MILCGERVDAATAERIGLVEQVVPTGEARNAALALAAKVAKQSPSSVTACKGLIQAAR
                     SNPMKHALPAERELFVDLFNTQDQREGVNAFLEKRAPHWKNA"
     gene            3276..4412
                     /locus_tag="PKN55_08720"
     CDS             3276..4412
                     /locus_tag="PKN55_08720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012695710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HNX71367.1"
                     /translation="MTVEHQDSACVIFTEHATPSGMKIGYATLNAEKSLNALSLEMIR
                     LLDARLRQWADDPQLACVVLQGAGEKAFCAGGDVRKLREAILAHEGPAAIPFASAYFA
                     EEYRLDYRIHTYAKPILLWGSGIVMGGGLGLMAGASHRVVTETSRIAMPEITIGLYPD
                     VGGSWFLPRMPGRLGLFLALTGAPLNAHDAQVVNLADWFVRSEDKLAIFAGLANIDWQ
                     ADPAHNHGLLSKLLRGFAQHAASHLPASQVRAHFDAIQHVTDGDSLAEVCEQITRHAS
                     DDAWMQRAAKSLAVGSPTSAALSWEIWRRSRLLGLADVFRMEWTLSVQCCAHPDFREG
                     VRALLVDKDNAPRWTPATLAEVSREWIDGHFVVPADTVHPLADL"
     gene            4485..5393
                     /gene="mmsB"
                     /locus_tag="PKN55_08725"
     CDS             4485..5393
                     /gene="mmsB"
                     /locus_tag="PKN55_08725"
                     /EC_number="1.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012695711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008442 - 3-hydroxyisobutyrate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006574 - valine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyisobutyrate dehydrogenase"
                     /protein_id="HNX71368.1"
                     /translation="MASIAFIGLGNMGGPMALNLLRAGHTLHVFDLSASAVQTLVDAG
                     AHAAASAAAAVAAADDAVISMLPASPHVKALYLGENGVLANARPGALLIDSSTIASHV
                     AVEVAQAAQAKGLRMIDAPVSGGTAGAAAGTLTFIVGGDAADLEAARPLLEAMGKNIF
                     HAGAAGAGQTAKICNNMLLGILMAGTAEALALGVANGLDPKVLSDIMSKSSGRNWALE
                     VYNPWPGVMENAPASRGYSGGFGSDLMLKDLGLAQEAALHARAATPLGAAARNLYQLH
                     SQNGQGGLDFSSIVQLMSAKAKPAQG"
     gene            complement(5560..6336)
                     /locus_tag="PKN55_08730"
     CDS             complement(5560..6336)
                     /locus_tag="PKN55_08730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018746920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HNX71369.1"
                     /translation="MTDTPPLLFDTHCHLDAAEFAADRDAQVAQARAAGVGEMLVPAV
                     EQANFADTLAMRARYGCRVALGLHPVYLPRHRPAHLEQLADWLDRERPDAVGEIGLDF
                     YLPELDPAQQEALFVAQLRLARARQLPVVLHLRRAQDRVLKYLRQVGVPGGIAHAFNG
                     SRQQAEAFIALGFKLGFGGAMTYSGSQRIRQLAASLPLSALVLETDAPDIRPAWAPDT
                     PNAPANLHRFAQVLAELRGEPLDEVIAATTANARAALLLR"
     gene            6466..6969
                     /locus_tag="PKN55_08735"
     CDS             6466..6969
                     /locus_tag="PKN55_08735"
                     /inference="COORDINATES: protein motif:HMM:NF022072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HNX71370.1"
                     /translation="MRGVSLRRALALAALFSAGVASAGELQVSVSQTLSASPEEVWQE
                     IGRFGQLSWHPAVATTVVRGNPERSGAVREVTTVDGAVLVEKLVAHAAGQHSMSYRIL
                     RSPLPVSGYVSTLRVLPAQFGGSKVVWSSHFRRTAAVDDAAARQAVEGIYRAGMQSLV
                     KAFGSPQ"
BASE COUNT         1206 a         2279 c         2276 g         1108 t
ORIGIN      
        1 gccagcggcg tggacaccta caccctgctg caaccgctgg gcgtgtgcgc cggcatcacc
       61 ccgttcaact tcccggcgat gattccgctg tggatgttcc cgatggccat cgccaccggc
      121 aacacctttg tgctcaagcc ctccgagcaa gacccgctgg tcaccatgaa gctggtcaag
      181 ctggcgctgg aagccggcgt gccgccgggc gtgctgaatg tgattcacgg cggcgaagac
      241 gcggtgaacg cgctgtgcga ccacccggac atcaaggcga tttcgtttgt gggctccacc
      301 cgcgtcggca cccatgtgta caaccgcgcc agcctgaacg gcaagcgcgt gcagtgcatg
      361 atgggcgcca aaaaccacgc cgtggtgatg ccagacgcca accgcgaaca ggcgctcaat
      421 caactggtcg gcgcggcgtt tggcgccgcc ggccagcgct gcatggccac cacggtggcg
      481 gtgctggtgg gcgcggcgcg cgagtgggtg ccggagctgg tggccaaggc gcaggggctg
      541 cgcgtgggcg ccggcaagga taacccggat gtggggccga tgatttcctg cgccgcccgc
      601 cagcgcgcgc tgaacctggt ggcgcaaggc ctggagcagg gcgcggtgct ggagctggac
      661 gggcgcggcg tgcaggtgga cggctacccc aacggcaatt tctttggccc gaccatcctg
      721 tccggggtca agccgggcat ggcggtgtac gaccaggaga tttttggccc ggtgctggta
      781 ctgaccgagg ccgacagcct ggacgaagcc atcgagctga tcaacagcaa ccccaacggc
      841 aacggcaccg ccatcttcac ccagaacggc gcggtggcgc gcaagttcca ggaagacatc
      901 gacgtcgggc aggtgggcat caatgtgccg attccggtgc cggtgccgct gttcagcttt
      961 accggctcgc gcggctccaa gctgggcgac ctgggcccgt acggcaagca ggtggtgatg
     1021 ttctacaccc agaccaagac catcacccag cgctggtttg acgacccggc gtcgatgggc
     1081 aaggtgaaca ccaccatcag cctgaagtaa aaccgccaag cgccgcnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnaaga ggtggggcgg gccgcgcaag cgcatggctt
     1261 tgcggctcgc ttcggcgaca gacataagac aacaataaaa tcagaaggac ccacccccat
     1321 ggatttttcc ctctctgacg accagcgcgc ctttcaggac gccgcgcgcg cctttgcccg
     1381 ccacgagctg gcgccgcacg cggcggactg ggacgccgag tcgatttttc cgcgcgaggt
     1441 gattgcccgc gctggcgagc tgggcttttg cgggctgtac accccggaaa cctacggcgg
     1501 actgggcctg tcgcggctgg acgccgccat cgtgtttgaa gaactggccg ccggctgcac
     1561 ctccaccacg gcgtatatca ccatccacaa tatggtgtcg tggatgttcg ccacctgggc
     1621 gcgcccggaa gtggccgaac gctgggcgcc gtttttggcc agcggcgaaa aactgggcag
     1681 ctactgcctg accgaaccca acgccggctc ggacgccgcc aatctgcgca cccgcgccga
     1741 gcggcgcggt gaccgctatg tgctgaatgg cagcaagatg tttatctctg gcgcgggcga
     1801 caccgacctg ctgctggtga tggcgcgcac tggcgaggcc ggccccaagg ggatttcggc
     1861 atttgccgtg ccggccgatc tgccgggcat tcactacggc aaaaaagaac acaaaatggg
     1921 ctggaacagc cagcccaccc gcgccatcac ttttgacaat gtcgaagtgc cggacgacca
     1981 cttattgggc gaagaaggcc agggcttcag catggcgatg aaaggcctgg acggcgggcg
     2041 catcaatatc gccacctgct cggtaggcac cgcgcaagcg gcgctggagc gcgcccaggc
     2101 gtatttgcac gagcgcaagc agtttggccg cgagctggcc gagtttcagg cgctgcaatt
     2161 caagctggcc gacatggcca ccgagctggt ggcggcgcgg caaatggtgc gtctggctgc
     2221 ctggaagctg gaccacggca gcgccgacgc caccacctat tgcgccatgg ccaagcgctt
     2281 tgccaccgat gtcggcttca aggtggccaa cgatgcgctg caattacacg gcggctatgg
     2341 gtatattcgc gaatatccac ttgaacgtca tgtgcgcgac aatcgcgtgc accagattct
     2401 ggaaggcacc aacgaaatca tgcgggtgat tgttgcccgc gccatcctgc gccctggcgg
     2461 cgccgaggac atccgatgac acactacccc cacctgttgc tggacattca acaacacacc
     2521 gcgctggtca ccatcagcaa cccgccggcc aacacctgga acgccgacag cctgaacggc
     2581 ctgatcgagc tggtgcacga actgaacgcc aaccgcgaca tctacgcgct ggtgctcact
     2641 ggcgagggca gcaagttttt ctcggccggc gccgacttga atatgtttgc cgacggcgac
     2701 ccggcggtgg cgcgcaaaat ggcgcggctg tttggccagg cgtttgaaac cctcaccgat
     2761 tttcgcggcg tgtcgattgc cgccatcaac ggctacgcca tgggcggcgg cctggaagtg
     2821 gcgctggcct gcgatttgcg cattgccgaa acccaggcgc aaatggcgct gccggaagcc
     2881 accgttggcc tcttgccctg cgcgggcggc acgcaaaacc tgccctggct ggtgggcgaa
     2941 ggctgggcca agcgcatgat tctgtgcggc gagcgggtgg acgccgccac cgccgagcgc
     3001 atcggcctgg tcgagcaagt ggtgcccacc ggcgaagcgc gcaacgccgc gctggcgctg
     3061 gcggccaagg tggccaagca aagcccgagc agcgtcaccg cctgcaaggg cctgatccag
     3121 gcggcgcgca gcaacccgat gaagcacgcg ctgccggccg agcgcgaatt gtttgtcgat
     3181 ttgttcaaca cccaggacca gcgcgaaggc gtcaacgcct ttctggaaaa acgcgccccc
     3241 cactggaaaa acgcctgatt gccccggaac gcgccatgac cgtagaacac caagactccg
     3301 cctgcgtgat tttcaccgaa cacgccacgc ccagcggcat gaaaatcggc tacgccacgc
     3361 tgaatgcgga aaaatcgctg aacgccctgt cgctggagat gatccgcctg ctggacgccc
     3421 gcctgcgcca atgggcggat gacccgcaac tggcctgcgt ggtgctgcaa ggcgctggcg
     3481 aaaaagcctt ttgcgctggc ggcgacgtgc gcaagctgcg cgaggccatt cttgcccacg
     3541 aaggcccggc ggccattccg tttgcttcgg cgtattttgc cgaagaatac cgcctggatt
     3601 accgcatcca cacctacgcc aagcccatcc tgctgtgggg cagcggcatt gtcatgggcg
     3661 gcggcctggg cctgatggcc ggcgccagcc accgcgtggt caccgaaacc tcgcgcatcg
     3721 ccatgccgga aatcaccatt ggcctctacc cggacgtggg cggcagctgg ttcctgccgc
     3781 gcatgcccgg ccggttgggc ttgttcctgg cgctgaccgg cgcgccgctg aacgcccacg
     3841 acgcccaggt ggtgaatctg gccgactggt ttgtgcgcag cgaagacaag ctggccattt
     3901 ttgccggcct ggccaatatc gattggcagg ccgacccggc gcacaaccac ggtttgctgt
     3961 ccaagctgct gcgcggcttt gcccagcacg ccgccagcca tctgccggcc tcgcaggtgc
     4021 gcgcccactt tgacgccatc cagcatgtca ccgacggcga cagcctggcc gaggtgtgcg
     4081 aacaaatcac ccgccacgcc agcgacgacg cctggatgca gcgcgccgcc aaatcgctgg
     4141 cggtgggctc gcccacctcc gctgcgctca gttgggaaat ctggcggcgc agccggctcc
     4201 tgggcctggc cgatgtgttc cgcatggaat ggacgctgtc ggtgcagtgc tgcgcccacc
     4261 cggacttccg cgaaggcgtg cgcgccctgc tggtggacaa ggacaacgcc ccgcgctgga
     4321 cgccggccac cctggccgag gtcagccgcg agtggatcga cggccatttt gtcgttcccg
     4381 ccgacaccgt ccacccgctg gccgacctgt aacttttggc cgcgcccgac cccacgccgc
     4441 agcagacggc gacggcgcgg cggcccccac aaggagacaa catcatggca agcattgcat
     4501 tcattgggct gggcaatatg ggtgggccga tggcgctcaa cctgctgcgc gccggccaca
     4561 ccctgcacgt ttttgacctg tccgccagcg cggtgcaaac cctggttgat gccggcgccc
     4621 acgctgccgc cagcgccgcc gccgccgtgg cggcggcaga cgacgcggtg atttccatgc
     4681 tgccggccag cccgcacgtc aaggcgctgt acctgggcga aaacggcgtg ctggccaacg
     4741 cccgccccgg cgcgctgctg atcgacagca gcaccatcgc cagccatgtc gccgtggaag
     4801 tcgcccaggc cgcccaggcc aagggcctgc gcatgatcga cgccccggtg tccggcggca
     4861 ccgccggcgc cgccgccggc acgctgacct ttatcgtcgg cggcgacgcc gccgacctgg
     4921 aggccgcccg cccgctgctg gaagccatgg gcaagaatat cttccacgcg ggcgccgccg
     4981 gcgccgggca aaccgccaag atttgcaaca atatgctgct gggcatcctg atggccggca
     5041 ccgccgaggc gctggcgctg ggcgtggcca atggcctgga cccgaaagtg ctgtccgaca
     5101 tcatgagcaa gagctcgggc cgcaactggg cactggaagt gtataaccct tggccggggg
     5161 tcatggaaaa cgcgcctgcc agtcgtggct acagcggcgg ctttggctcg gatttgatgc
     5221 tcaaggatct gggtctggcg caagaggcgg ccttgcacgc ccgcgccgcc accccgctgg
     5281 gcgcggcggc gcgcaatctg taccagctgc acagccagaa cggccagggc gggctggatt
     5341 tttccagcat cgtgcaactg atgagcgcca aggccaagcc cgcacagggt tgagttgata
     5401 ttgcatcttc ccccgtcacg gccttgcgtg gcacaaggcg cacgcggctt gccgcgcgtg
     5461 acgggcggga tctgggccgg cgggtgacca agagggggga gccgccggtt ttttttgcct
     5521 gatgcacagg atcgctgcgc tgggtgttgt gagtgactct cagcgcaata acaaggccgc
     5581 ccgcgcattg gccgtggtgg cggcaatcac ctcatccagt ggttcgccgc gcagctcggc
     5641 cagcacctgg gcaaagcggt gcagattggc gggcgcattc ggcgtgtctg gcgcccaggc
     5701 cgggcggatg tcgggcgcgt cggtttccag caccagcgcc gacagcggca agctggcggc
     5761 cagttggcgg atgcgttgcg aaccgctgta ggtcatcgcc cccccaaatc ccagcttgaa
     5821 acccagcgcg ataaacgcct cggcctgttg ccggctgccg ttgaaggcgt gggcaatgcc
     5881 gcctggcacg cccacctggc gcaggtattt cagcacccga tcctgcgcgc ggcgcaagtg
     5941 cagcaccacc ggcagctgac gcgcgcgcgc cagccgcagt tgggcgacaa acagcgcctc
     6001 ttgttgggcg ggatcgagtt ccggcaggta aaaatccagg ccgatttcgc ccacggcatc
     6061 ggggcgttcg cggtccagcc agtcggccag ctgttcgaga tgcgccggcc gatggcgcgg
     6121 cagatacacc gggtgcaggc ccagcgccac ccggcagccg tagcgcgcgc gcatggccag
     6181 ggtgtcggca aaattggcct gttcgaccgc cggaaccaac atctcgccca cgccagcggc
     6241 gcgcgcctgc gccacttgcg cgtcgcggtc ggcggcaaac tcggcggcgt ccaggtggca
     6301 atgggtgtca aacagcaagg gtggggtgtc agtcatgtgc atacggccaa tctagcgcac
     6361 ccagggcctg ccggccagtt gcgacttaag ttgcatacgc ggttggccaa cggctgccta
     6421 gaatggggat gttttgttca tcaacacccc caacaggagt tttccatgcg tggagtatcc
     6481 ttgcggcgcg ccctggcgct ggccgccttg ttcagcgctg gcgtcgccag tgctggcgaa
     6541 ttgcaggtca gtgtcagcca gaccctatcg gccagcccgg aagaagtctg gcaggaaatc
     6601 ggccgctttg gccagttgag ctggcacccg gcagtggcaa ccacggtggt gcgcggcaac
     6661 ccggaacgca gcggcgccgt gcgggaagtc accacggtgg atggtgcggt gctggtggaa
     6721 aagctggtgg cgcatgcagc cggccagcac agcatgagct atcgcatcct gcgttcgcca
     6781 ttgccggtca gcggctatgt ctccaccttg cgggtgctgc cagcccagtt tggtggcagc
     6841 aaggtggtgt ggtcgtcgca tttccggcgc acggcggccg tggatgatgc tgcggcacgc
     6901 caggccgtag agggcattta tcgcgcgggc atgcagtcgc tggtcaaggc cttcggcagc
     6961 ccgcaataa
//
LOCUS       DAOPYX010000153         6942 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_13288_length_6942_cov_8.645274, whole genome shotgun sequence.
ACCESSION   DAOPYX010000153 DAOPYX010000000
VERSION     DAOPYX010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 6942)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6942)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6942
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13288_length_6942_cov_8.645274"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            154..726
                     /locus_tag="PKN55_08740"
     CDS             154..726
                     /locus_tag="PKN55_08740"
                     /inference="COORDINATES: protein motif:HMM:NF013964.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HNX71371.1"
                     /translation="MDYLSLFRMPTPMKITGRSSSITNAFINSIIPVVPPCAEEVQQA
                     LKILGMTHENFQCAYCGGVASEWDHLRPLVKDKKPTGYISEIHNLVPSCGKCNQSKGN
                     KEWKTWMLSTARLSPTARGIKDISARVKRLEAYENSKAPTKMDFSAIVGQKVWAQHQD
                     NLERIQSLMRESQELATKINAKVASAYKAL"
     gene            complement(1133..1489)
                     /locus_tag="PKN55_08745"
     CDS             complement(1133..1489)
                     /locus_tag="PKN55_08745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX71372.1"
                     /translation="MNVRLCAPLLAALSLSPLALADTPAQRCQDLLESGMRMAGWEQH
                     CQFNAGVAPALRAAYMQAGCPGIIKDDTVDRTVRQLDAELNGQIQRAGSEAAFCTAVR
                     PAYEDIVGAMPSSQSR"
     gene            complement(1516..1965)
                     /locus_tag="PKN55_08750"
     CDS             complement(1516..1965)
                     /locus_tag="PKN55_08750"
                     /inference="COORDINATES: protein motif:HMM:NF020551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1857 family protein"
                     /protein_id="HNX71373.1"
                     /translation="MNDLNNPMATTLSRQVLWRGLVRRAERPQDFLPDLAHGDIIARG
                     EDWFDRIVRLGTLHVRDRVTLQAEHSLRHDTAAGDGYPASTLTISIEEPEPLSLFVRF
                     VYQSTLPEHTPPGSEVDYVAYLKSAYQQMDTEAIATLRQLAEAGELD"
     gene            2138..2359
                     /locus_tag="PKN55_08755"
     CDS             2138..2359
                     /locus_tag="PKN55_08755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HNX71374.1"
                     /translation="MNRMLSIGVCALLLTASLTGCADMNKRQRNTAIGAAVGGVAGSV
                     LTGGSTVGTVGGAVVGGVIGSQGDSGKKK"
     gene            2642..3262
                     /gene="hybD"
                     /locus_tag="PKN55_08760"
     CDS             2642..3262
                     /gene="hybD"
                     /locus_tag="PKN55_08760"
                     /inference="COORDINATES: protein motif:HMM:TIGR00140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009375 - ferredoxin hydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HNX71375.1"
                     /translation="MADSSHIVILGIGNLLWADEGFGVRAVEALHAGWQWPAEVELVD
                     GGTQGLALLPIVQAASHLLILDAIDAGLAPGELAVFADDAVPAYLTAKKMSLHQTGFA
                     EVLALAELSGQSPCRLALVGVQPVELEDYGGSLTAPVRAQLPLALARALDILAGWGVY
                     PAARQAPAADGLHDASLALLPYEAGRPSAEAACRVGDARVLAQGEG"
     gene            3266..3586
                     /locus_tag="PKN55_08765"
     CDS             3266..3586
                     /locus_tag="PKN55_08765"
                     /inference="COORDINATES: protein motif:HMM:TIGR00074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HypC/HybG/HupF family hydrogenase formation
                     chaperone"
                     /protein_id="HNX71376.1"
                     /translation="MCVGVPMQVLRLESPFAVWCRDASGGEALIDIGLIAAPQPGDWL
                     LTFLGAAREVIDEQTAAQIQSALAAVAAALDGEHDLDAHFADLINREPQLPEFLLPAK
                     ESAA"
     gene            3583..4035
                     /locus_tag="PKN55_08770"
     CDS             3583..4035
                     /locus_tag="PKN55_08770"
                     /inference="COORDINATES: protein motif:HMM:NF019092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX71377.1"
                     /translation="MTAPVSVSAALPALLSRLSARGWPLLSVDALADFARQHGEVVAF
                     CVDDPTKLPEVADAAVILPELLKDSGLPLTPCVVDPAGNAELQARYGVMKYPAFLFLR
                     DGGYVGVMAGLRSWGEYLHEVNEMRARPVGRAPSIGIKVAGPNAGGCA"
     gene            4032..4892
                     /locus_tag="PKN55_08775"
     CDS             4032..4892
                     /locus_tag="PKN55_08775"
                     /inference="COORDINATES: protein motif:HMM:NF016682.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation C-terminal
                     domain-containing protein"
                     /protein_id="HNX71378.1"
                     /translation="MKPFPLPVVALGAGSQPEDPDLAYMPLPGEVESFIMPALPSSED
                     VSHLGRAIELIGQLAALAARWQPGERCVRELAGLNSEELALVNQVLGEGEVSAKALLA
                     DGGEARIQESVFAGVWRVCRFDANGQQTGDSVEVAPIPELLIERAQAASRWLDALPPM
                     PATLMNAAPIGAELLARQRQALDSPHVINFTLLPVTPDDLAWITDLVGEGAVGIFSRG
                     YGKCRVLATALTHVWRVQYFNTMNTLLLDTLEITRIPDVTLADGEDIADSAERLGEAH
                     HWLCGAFDHV"
     gene            4885..5091
                     /locus_tag="PKN55_08780"
     CDS             4885..5091
                     /locus_tag="PKN55_08780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017508555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HNX71379.1"
                     /translation="MFEGSYLGDAGRLPADARLECKICWWVYDPAQGDDVWQIPPGTP
                     FAELPAHWKCPVCDGARDQFMVLE"
     gene            5088..5531
                     /gene="hybE"
                     /locus_tag="PKN55_08785"
     CDS             5088..5531
                     /gene="hybE"
                     /locus_tag="PKN55_08785"
                     /inference="COORDINATES: protein motif:HMM:TIGR03993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[NiFe]-hydrogenase assembly chaperone HybE"
                     /protein_id="HNX71380.1"
                     /translation="MIHHGNPSARLREVFSRIQAERMADVPLLNPALTVETVGFRLWQ
                     DSWLGVLITPWSMNLLALAGVTPFAPLGAHAVDSLALPDGAVDFHGAFEPALGHYRQA
                     SLFSPMWQFAHQDAARATAEEVMNLLFPAAPAPDLSRRRLFGLRA"
     gene            5528..6571
                     /locus_tag="PKN55_08790"
     CDS             5528..6571
                     /locus_tag="PKN55_08790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX71381.1"
                     /translation="MTLEHLAGRLTLTLTHDGAAVTHCHSEFSRPPLAARLLAGAAPA
                     DAAARLAPLFAVCRRAHALAADLAAQALHGPAAPRPEALAELEAEARQDILRRVALDW
                     PLACGDSPDADFVAAVRAQPAALGQALQDYVLGEPCADWLARGYHGWLAWARQRHTQY
                     AWRLADLMAESCGGMPLLSFPSEGELAALCRELLDDDQFAQTPSWVGSPVETGPLARQ
                     ANWVASLFAAEYWPAARMLARLIELVRLSQGSAGYQARAVALDPQRAVAAVDTARGLL
                     LHAVAVDAHGQVADWRILPPTAWNFHPEGAWAAALDRVLPEQAEAAARRAALWLDPCV
                     EYQLMIKDSRQYA"
     gene            6564..6905
                     /gene="hypA"
                     /locus_tag="PKN55_08795"
     CDS             6564..6905
                     /gene="hypA"
                     /locus_tag="PKN55_08795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011516275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HNX71382.1"
                     /translation="MHEMSLAESIVSLVESHAEAEGFSRVKAIWLEIGELAGVEVEAL
                     TFSLDVVMRESLMEGAQVHIRRQPAQGWCLACSQAVEVRDRVSGCPLCGSHQLQVSGG
                     EALRVAELEVE"
BASE COUNT         1124 a         2123 c         2397 g         1298 t
ORIGIN      
        1 gttgatgctg ccgcacctgc ctgatgttat tggcatggtt cagaacatgc tctaagaatt
       61 tttatatgcc gtatataccc aacccatcat tccatcggac ttgcgcgaag agcggtacag
      121 gccggtgagt tcagacgtta gagagcaatc ctaatggact atctgtcact attcagaatg
      181 ccaaccccga tgaaaatcac ggggcgtagc tccagcataa cgaacgcctt catcaattcg
      241 ataattccgg ttgtgccgcc ctgcgcggaa gaggttcagc aggcattgaa aatcctcgga
      301 atgacccacg aaaattttca atgcgcatac tgtggtggcg ttgcgtcgga atgggatcat
      361 cttcgcccgc ttgtcaaaga caagaaacca actgggtaca tctcagaaat tcacaacctc
      421 gtgccatctt gtggaaagtg caaccaatca aagggtaaca aagaatggaa aacctggatg
      481 ctcagtactg cgagattatc cccaacagca cgcggcatca aagacatctc cgcacgagtc
      541 aagcggctgg aggcatacga aaactccaag gctccgacaa aaatggattt ttcagccatc
      601 gtaggacaga aagtttgggc gcagcaccaa gacaatctgg agcggattca gtccctcatg
      661 cgcgaatcgc aggaactcgc tacaaagatc aatgctaagg ttgctagtgc atacaaagcg
      721 ctctaacccg gcactcgtcc tcgctctctt cagtcgctgg acgctgcgca ataaaccgcc
      781 acgcagcatg tcgagaatat cttagggtct tcattgcaag cctactttgg ccggtggatt
      841 ggtggttatt gcgctgttct tgggtgcggc ttcgtggctc caaactagta gccagtcacc
      901 atgatctgaa cagattgact ggcgcttaga ccgctttcgg catgagtttc atccccggtg
      961 tcgttcccgc gaaggcggga acccagacgg cgccacagcg tgcgcggtgg gcatggcgct
     1021 ggtgagcgct caccccgcta tacgctgtgg gcacacctgg attcccgcct gcgcgggaat
     1081 gactcggtat agcgcggcga gtggttcttt gtggcacatc aaccctcgtg tttcaacgtg
     1141 actggctact cggcatcgcc cccacaatat cctcatacgc cggccgcacg gccgtgcaaa
     1201 acgccgcttc gctgccggcg cgctggattt gcccgttcag ttcggcgtcc agttggcgca
     1261 cggtgcggtc taccgtgtcg tccttgatga tgccggggca gccggcttgc atataagcgg
     1321 cgcgcagggc gggggcgacg ccggcgttaa attggcagtg ttgttcccag ccggccatgc
     1381 gcatgccgct ttccagcaaa tcctggcagc gttgcgcggg ggtgtcggcc agggcgagtg
     1441 gggataggct tagcgccgcc agcagcggcg cgcacaggcg aacattcatc gtggcaatcc
     1501 ttgcggggtg aggggtcaat ccagttcgcc ggcttcggcc agttggcgca gggtggcgat
     1561 ggcttcggtg tccatttgct gataggcgga tttcaggtag gccacgtaat ccacctcgct
     1621 gcctggcggg gtgtgctcgg gcagggtgga ttgatacaca aagcgcacaa acagcgacaa
     1681 gggttcgggt tcttcaatgc tgatggtcag cgtgctggcc gggtagccat cgccggcggc
     1741 ggtgtcgtgg cgcaggctgt gttcggcttg cagggtgacg cggtcgcgca cgtgcagggt
     1801 gcccaggcgc acgatgcggt cgaaccagtc ttcgccacgg gcgatgatgt cgccgtgggc
     1861 caggtcgggc agaaaatcct gcggccgctc ggcgcggcgc accaggccac gccatagcac
     1921 ctggcgcgag agggtggtgg ccatggggtt gttcaggtcg ttcacctgga taacatgctc
     1981 gaagtacaca acagtccttt atttggctgg acgcaaaaca gccgccagtg taaaaccatt
     2041 cgccgcacaa agggaggccg ccggaacttt ttgcccgacg gcgcggccct atttgtggct
     2101 ttgattaacg gcgcgcgacg ccgggaaagg atgttcgatg aatcgcatgc tgagtatcgg
     2161 ggtgtgtgcc ctgttgctga ccgccagcct gacgggctgc gccgacatga acaaacgcca
     2221 gcgcaacacc gccattggcg ccgccgtggg cggcgtggcc ggctcggtct tgaccggcgg
     2281 cagcacggtg ggcaccgtgg gtggcgcggt ggtgggcggc gtgattggca gccagggcga
     2341 cagcggcaag aaaaaataat cccggctggc cgctcggcca gccttcacgc agcggggtga
     2401 cggcatggcc gcaccctgct gatatggtgt gatgccagat tatcctgctg gcaagtggtt
     2461 atctgtcttg cagttggctg ataaaccgca aagcacataa ccgcaaaaat cctcaggctt
     2521 tccctttctg ttccgccagt ggcctgattt cacgccacaa ttcccgttct cctgctttaa
     2581 ccggccatgg cacgctttgt gtctgtgtta cgccatctgt aacacgtctg ggcctgtgca
     2641 catggcggat tcttctcata ttgtcattct gggcatcggc aacctgctgt gggccgacga
     2701 gggctttggc gtgcgcgcgg tagaggcgct gcacgcgggc tggcagtggc cggccgaggt
     2761 cgagctggtg gatggcggca ctcaggggct ggcgctattg ccgattgtgc aggcggccag
     2821 ccatttgctg attctggacg ccatcgacgc cggtctggcg ccgggcgagc tggcggtgtt
     2881 tgccgacgat gccgtgccgg cctatctcac cgccaaaaaa atgagcttgc accaaaccgg
     2941 ctttgccgag gtgctggcgc tggccgagtt gtctggtcag tcgccgtgcc ggctggcgct
     3001 ggtgggcgtg cagccggtgg agctggaaga ctacggcggc agcctgactg cgccggtgcg
     3061 cgcgcagttg ccgctggcgc tggcgcgcgc gctggacatc ctggccggct ggggcgtgta
     3121 tcccgccgca cgccaggcgc cggccgccga cggcctgcac gacgccagcc tggcgctgtt
     3181 gccctacgaa gccggccggc ccagcgccga ggccgcctgt cgggtgggcg atgcgcgcgt
     3241 gctggcgcaa ggggagggtt aagccatgtg tgtgggtgtg cccatgcaag tcttgcggct
     3301 ggaatccccg tttgccgtgt ggtgccgcga cgccagcggc ggcgaagcgc tgatcgacat
     3361 cggcctgatt gccgcgccgc agccgggcga ctggctgctg acctttttag gcgcggcgcg
     3421 cgaggtgatc gacgaacaga ccgccgcgca gattcagtcc gcgctggcgg cggtggccgc
     3481 cgcgctggac ggcgaacacg atctggacgc gcactttgcc gatttgatca accgcgaacc
     3541 gcaattgccc gaatttttac tgcccgccaa ggagtccgct gcgtgactgc ccctgtttct
     3601 gtttctgccg ccctgccggc tttgttgtcg cgcctgagcg cgcgcggctg gccgctgctc
     3661 agcgtggatg cgctggccga ttttgctcgc cagcacggcg aagtggtggc gttctgtgtg
     3721 gacgacccca ccaagctgcc ggaagtggcc gacgcggcgg tgattttgcc cgagctgctg
     3781 aaagacagcg gcctgccgct caccccctgt gtggtcgatc ccgccggcaa cgccgagctg
     3841 caagcgcgct acggggtgat gaaatacccg gcgtttttgt ttttgcgcga tggcggctat
     3901 gtcggcgtga tggccggcct gcgcagctgg ggcgaatacc tgcacgaagt caacgagatg
     3961 cgcgcccgcc cggtgggccg cgcgcccagc atcggcatca aggtggccgg cccgaatgca
     4021 ggaggctgcg catgaaaccg tttcctctgc ctgtggtggc cctgggcgcg ggctcgcaac
     4081 cggaagaccc ggatttggcc tatatgccgc tgcccggcga agtcgagagc tttatcatgc
     4141 cggcgctgcc cagttcggaa gacgtcagcc acctgggccg cgccattgaa ttgattggcc
     4201 agttggccgc gctggcggcg cgctggcagc cgggcgagcg ctgcgtgcgc gagctggccg
     4261 ggctgaacag cgaagagctg gcgctggtga atcaggtgct gggcgagggc gaagtcagcg
     4321 ccaaggcgct gctggccgac ggcggcgagg cgcgcattca ggaatcggtg tttgccgggg
     4381 tgtggcgggt gtgccgcttt gacgccaatg gccagcaaac cggcgacagc gtggaagtgg
     4441 cccccattcc cgagctgctg atcgagcgcg cccaggccgc cagccgctgg ctggacgcgc
     4501 tgccgccaat gccagccacg ctgatgaacg ccgcgcccat cggcgccgag ctgctggccc
     4561 gccagcgcca ggcgctggac agcccgcatg tgatcaactt caccctgctg ccggtcaccc
     4621 cggatgacct ggcctggatc accgatttgg ttggcgaagg cgcggtgggg attttttcgc
     4681 gcggctatgg caaatgccgg gtgctggcca ccgcgctgac ccatgtctgg cgggtgcagt
     4741 atttcaacac catgaacacc ctgctgctgg acaccctgga aatcacccgc atccccgatg
     4801 tgacgctggc cgacggcgag gacatcgccg attctgccga gcggctgggc gaggcgcacc
     4861 actggctgtg cggagcgttt gaccatgttt gaaggcagct acctgggcga cgccggccga
     4921 ctgccggccg acgcccggct ggaatgcaaa atctgctggt gggtgtacga cccggcgcag
     4981 ggcgacgatg tctggcagat tccgcccggc acgccgtttg ccgagctgcc ggcgcactgg
     5041 aaatgcccgg tgtgcgacgg cgcgcgcgac cagttcatgg tgctggaatg atccatcacg
     5101 gcaatccttc cgcccgtctg cgcgaggtgt tcagccgcat ccaggccgag cgcatggccg
     5161 acgtgccctt gctcaatccg gcgctgacag tggagacggt gggctttcgc ttgtggcagg
     5221 atagctggct gggcgtgctg atcaccccgt ggagcatgaa tctgctggcg ctggccgggg
     5281 tcaccccgtt tgcgccgctg ggcgcgcacg cggtggacag cctggccctg cccgatggcg
     5341 cggtggattt ccatggcgct ttcgagccgg cgctcggcca ttaccgccaa gcttcgctgt
     5401 tttcgccgat gtggcaattt gcccatcagg acgcagcgcg ggccacggcc gaggaagtga
     5461 tgaacctgct gtttccagcc gcgcccgcgc cggatttgtc gcggcggcgg ctgtttggat
     5521 tgcgcgcatg acccttgaac acctggctgg ccgcctgacc ctgacgctga cccacgacgg
     5581 cgccgccgtc acccattgcc atagcgaatt ctcccgcccg ccgctggccg cccgcctgct
     5641 ggccggcgct gcccccgctg acgccgccgc ccggctggcg ccgctgtttg ccgtgtgccg
     5701 ccgcgcccac gcgctggccg ccgacctggc cgcccaggcg ctgcacggcc cggcggcgcc
     5761 ccgccccgag gcgctggccg agctggaagc cgaagcccgc caggacatcc tgcgccgggt
     5821 ggcgctggat tggccgctgg cctgcggcga cagcccggac gccgactttg tcgccgccgt
     5881 gcgcgcccag cccgccgcgc tgggccaggc gctgcaagac tatgtgctgg gcgagccctg
     5941 tgccgactgg ctggcgcgcg gctatcacgg ctggctggcc tgggcgcgcc agcgccacac
     6001 ccaatacgcc tggcggctgg cggatctgat ggccgaatcc tgcggcggca tgcccttgct
     6061 gagttttccg agcgaaggcg agctggccgc gctgtgccgc gagctgctgg acgacgatca
     6121 atttgcccaa acccccagct gggtgggcag cccggtcgaa accggcccgc tggcgcgcca
     6181 ggccaattgg gtggccagcc tgtttgccgc cgaatactgg ccggccgcgc gcatgctggc
     6241 gcggctgatt gagctggtgc ggctgagcca gggcagcgct ggctatcagg cccgcgcggt
     6301 ggcgctggac ccgcagcggg cggtggcggc ggtggacacc gcgcgcggcc tgttgctgca
     6361 cgcggtggcg gtggacgccc acggccaggt ggccgactgg cgcatcctgc cgcccaccgc
     6421 ctggaatttt catcctgaag gggcctgggc ggcggcgctg gaccgcgtgt tgcccgagca
     6481 ggccgaagcc gccgcccgcc gcgccgcgct gtggctggat ccgtgtgtgg agtatcaatt
     6541 gatgatcaag gatagccgtc aatatgcatg agatgtcctt ggcggaaagc attgtcagcc
     6601 tggtggaaag ccacgccgag gcggaggggt tcagccgggt gaaggcgatt tggctggaga
     6661 ttggcgagct ggccggggtg gaggtcgagg cgctgacgtt tagtctggat gtggtgatgc
     6721 gcgagagctt gatggaaggc gcgcaggtgc atatccgccg ccagccggcg cagggctggt
     6781 gtctggcgtg ttcgcaggcg gtggaggtgc gggatcgggt gagtggttgc ccgctgtgcg
     6841 gcagtcatca gttgcaggtg agtggtggcg aggcgttgcg ggtggcggag ttggaggtgg
     6901 agtgaggggg taggtaggcg gtgggttgcg ctggcgcgca gg
//
LOCUS       DAOPYX010000154         6841 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_13544_length_6841_cov_11.582818, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000154 DAOPYX010000000
VERSION     DAOPYX010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 6841)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6841)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6841
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13544_length_6841_cov_11.582818"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..1221
                     /locus_tag="PKN55_08800"
     CDS             1..1221
                     /locus_tag="PKN55_08800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004285373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNX71383.1"
                     /translation="MLRSLRVLNTPLSLAITLSAAGVLMITMGARQSLGLFVSPIHDS
                     TGMGVAAISLALAVGQFAWGAIQPLAGALADRHGPRGVLWVGVLTLALGCALTPWVSS
                     GFGLTVTLGLLAAMGSGVGSFSVLIGAAAQQLPAQARGAASGVINAGGSFGQFIFAPI
                     LQGLIQRLGWMGAMWGMAAMTLLALPMVGRLTRGYQPPPAPSAAEGGVLRAIREAMAD
                     RSYLLLHLGFFTCGFHIAFLVTHLPGEVNLCGLPPSVASWSLAIIGLSNIVGSLYAGA
                     CVSRYRSKYVLAVMYGSRALLIALYLMAPATAWTYYLFAAGLGLTWLATVPPTAAIIG
                     KLFGVRYLGTLFGLTLLSHQTGGFLGAWLGGLAITHLGDFTWMWYADMALAALAAVIN
                     LPIREQPVWQAQPA"
     gene            1401..2516
                     /locus_tag="PKN55_08805"
     CDS             1401..2516
                     /locus_tag="PKN55_08805"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNX71384.1"
                     /translation="MKPVFTLAMLGLLACGLSACQPESTPAKKSGPPAVTITTQPLSQ
                     RPLEARIASLASIEAVNSPQVLAEVAGRVAAVAVDVGTQVKPGQLLLTLDPADLRNSK
                     LAQDAEVARLGALADQQRRNVVRYRDLLTQGFISDAKMDELVAQQQAIEQQWAAAKAQ
                     AANSQRMVDKAQVRAPVAGAIDTRLVNVGEYVTVGKPLFTLASGGALRARFALSGREA
                     ASVKPGLLVRLEADGERLSSRISETRPAIDARNGMIEALAPLPADGPWRAGLAVHAEV
                     VLAERQGLAAPQVAVIERPAGSTVYVVKGDRVEARRVRTGIRQDGWVELLDGVQAGET
                     LAVDGAGFLTDKAKVKLAKPDKDKSGKDKASKAEAKS"
     gene            2513..5548
                     /locus_tag="PKN55_08810"
     CDS             2513..5548
                     /locus_tag="PKN55_08810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008954719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNX71385.1"
                     /translation="MKLAELSIRRHVLAYMLSAVLVLFGMIAYQKIGVDRYPNIEQPV
                     ISIATTLTGATPEVMDSSVTQIIEAAVNSVPGIDSIESTSQPGRSTVKVTFNLDKNID
                     VAFNEVQVKVNQAQRRLPDDVDMPVVSKTDANGSPIVWLALTGDRTEKQLYTYANTVV
                     KKQLETVDGVGEVVLRGRGARVIRVELDPVKLAGYNLTPAEVKSAFGREHLQQPGGTV
                     VNGPAELLVNLDLEFKSVRALQDLVVAWRGGGAIRLADVARVIDGEGDLRSLTRFNGK
                     PAVLVGIVRVANSNTVALSQAVIERVDGQIRPALPPGIELTLAANDASFVVEMIGALK
                     DHLFEGTLLAGLIVWLFLRSLRSTLIISLAIPVSLLGAVAVMYFLGYTFNTVTLLGLL
                     LLIGVVVDDAIVVLENIHRLREQGHDNPIEAAILGSQQVVFAVIAATLSLVAIFVPVA
                     FLGGIVGRLFNSFALVVACGVLVSLFVSVTLTPMLCSRFLKVEKQHGRVYVWLERGFQ
                     AMENGYRRVLDWTLRHRGKVLGFALLSVTASVAVFMLLKSEFLPEEDESRFLVNIKTP
                     IGASIHYTNQKSREVEAILLRHGEVRNLLTIVGGFSGTQVNQATFVVRLTARHTRTVR
                     QGELLAKVRKELGHVPGIRASAFPIPRVGESRGGKLQFAVIGPNLTDVAKHADALMQR
                     LGSQPGIGRLDIDLDLEQPQLSFDIDRAAAARVGVSTESIATALNLLTAGSNVAKFSD
                     GDGERYDVRVKAADMAISRAEDLNAIYLRSQNGELVRLDAVVRVKRTLGPTLIARQNL
                     QYAANLKGSPTLALAEAVAEVQATARDILPPGYRVEMQGEARELARGGGQAVFIFGLA
                     SLLLYMILASQFNSFAQPAIIMLAEPLAVVGGLLLLWLSGHTLNVYSMIGLILLIGLV
                     AKNSILLVDVTNQFRAAGHAVDDALRASCPLRLRPVLMTSLTVILAMLPAALGLGAGA
                     ETNGPLAVAVIGGMISSTLLTLAVVPAAYSLLENRRQAQ"
     gene            5640..>6841
                     /locus_tag="PKN55_08815"
     CDS             5640..>6841
                     /locus_tag="PKN55_08815"
                     /inference="COORDINATES: protein motif:HMM:NF015894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FapA family protein"
                     /protein_id="HNX71386.1"
                     /translation="MSEYEEEMNQSPFGLTMTETEAGELRLMFEPDDFRFKIDATALR
                     HMAIEKGYGELDFDENALAVAAQHVCKNEAFVSTIAQRRNAEYRVYVSSDRLVAQLQV
                     TPSYGGTPISVDAARNELAKARVVFGFDEAAFQSALTNKLGEPVTVAKGVAPIPGRDT
                     RFEPLVEVNRERVPRANEDGRVNWRDLGDIPMVSPGDVLMRRHPPEQGKSGRDVFDNI
                     LQAPDGKNLDYPYRLEGAERDANDPDLLRATSTGQPVLLRDSVKVEPVIKTPGVNVST
                     GNITFVGSLVINGDVQSGMHVKVDGDVTVHGTVEAAIIEAGGSITVNGGIVGQNAQQR
                     ESNANGEQAKLHAKANVRAKYAENALIIAEQSVYIDEAVIECDITALNRVEIGVRGRR
                     KGYIMGGRV"
BASE COUNT         1185 a         2043 c         2337 g         1276 t
ORIGIN      
        1 atgttgcgtt ctttgcgcgt gcttaacacc ccgctgtcgc tcgccatcac cttgtccgcc
       61 gctggcgtgc tgatgatcac catgggcgcg cgccagtcgc tggggctgtt tgtcagcccg
      121 attcacgaca gcaccggcat gggcgtggcc gccatcagcc tggcgctggc ggtggggcag
      181 tttgcctggg gagcgattca gccgctggcc ggcgcgctgg ccgaccgcca tggcccgcgc
      241 ggcgtgctgt gggtgggcgt gctcaccctg gcgctgggct gcgcgctgac gccgtgggtc
      301 agctcgggtt ttggcctgac ggtcaccctg ggcttgctgg cggcgatggg ctcgggggtg
      361 ggcagttttt cggtgttgat cggcgcggcg gcgcagcagc tgccggcgca ggcgcgcggc
      421 gcggcgtctg gggtgatcaa cgccggcggc tcgtttggcc agtttatctt tgccccgatt
      481 ctgcaagggc tgatccagcg cctgggctgg atgggggcga tgtggggcat ggcggcgatg
      541 acgctattgg cgctgccgat ggtgggccgg ctgacgcgcg gctaccagcc gccgcccgcg
      601 cccagcgcgg cggagggcgg ggtgttgcgc gccatccgcg aggcgatggc cgaccgcagc
      661 tatctgctgc tgcacctggg gtttttcacc tgcggctttc atattgcctt tttggtcacc
      721 cacctgcccg gcgaggtgaa tctgtgcggc ctgccgccgt cggtggccag ctggtcgctg
      781 gccattatcg gcctgtcgaa tattgtcggc agcctgtacg ccggggcgtg cgtgtcgcgc
      841 taccgcagca aatatgtgct ggcggtgatg tatggctcgc gcgcgctgct gattgcgctt
      901 tacttgatgg ccccggccac cgcctggacc tactacctgt ttgccgccgg cctggggctg
      961 acctggctgg ccaccgtgcc gcccaccgcc gccatcatcg gcaagctgtt tggcgtgcgc
     1021 tacctgggca cgctgtttgg cctgactttg ttgtcgcacc agaccggcgg ttttctcggc
     1081 gcctggctgg gtgggctggc catcacccat ctgggggatt tcacctggat gtggtacgcc
     1141 gacatggcgc tggcggcgct ggcggcggtg atcaatctgc cgattcgcga acagccggtc
     1201 tggcaggcgc agccggcata aaaaccgcgc atccacacgg ccaggcgcag ccatcggcga
     1261 tgcccgccca aaccaccggc gggcctgccc gcgctgtcgg catgtcatct gttcgcgtta
     1321 cactgcgcgc ttgttgacct gcggccaccg gccccaggct ttcggatgca acccaggcat
     1381 cctgcataca ggcgcgcttc atgaaacccg tttttaccct tgccatgctc ggcctgctgg
     1441 cctgcggact gagtgcttgc cagcccgaaa gcacaccagc caaaaaatcc ggcccgccgg
     1501 ccgtgaccat taccacccag ccactgagcc agcgcccgct ggaagcccgc atcgccagcc
     1561 tggccagcat cgaggccgtc aacagcccgc aagtgctggc ggaagtggcc gggcgcgtgg
     1621 cggcggtggc ggtggatgtc ggcacccagg tcaaacccgg ccagctctta ttgacattgg
     1681 atccggccga tttgcgcaac agcaagctgg cgcaagatgc cgaagtggcc cggctgggcg
     1741 cgctggccga ccagcagcgg cgcaatgtgg tgcgctaccg cgatttattg acccagggct
     1801 ttatctccga cgccaaaatg gacgagctgg tggcccagca acaggccatc gagcagcaat
     1861 gggcggcagc caaggcgcag gcagccaata gccagcgcat ggtggacaaa gcccaggtgc
     1921 gcgcgccggt ggccggggcg attgacaccc gattggtgaa tgtcggcgag tatgtgacag
     1981 tgggcaagcc cttgttcacc ctggccagcg gcggcgcgct gcgcgcgcgc tttgctctgt
     2041 ccgggcgcga ggcggccagc gtcaagccgg gcttgctggt gcgcctggag gccgacggcg
     2101 agcgcttgag ctcgcgcatc agcgaaaccc gcccggcgat tgacgcgcgc aacggcatga
     2161 tcgaagcgct ggccccgctg ccggccgatg gcccctggcg cgccgggctg gcggtgcacg
     2221 ccgaggtagt gctggccgag cgccagggcc tggcggcgcc gcaggtggcg gtgattgagc
     2281 gcccggccgg cagcacggtg tatgtggtga aaggcgatcg agtggaggcg cggcgcgtac
     2341 gtaccggcat tcggcaggat ggttgggtcg agctgctgga cggcgtgcag gccggcgaaa
     2401 ccctggcggt ggacggcgct ggctttctga ctgacaaggc caaggtgaag ctggccaaac
     2461 cggacaaaga taagagcggc aaggacaagg ccagcaaggc ggaggctaaa tcatgaagct
     2521 ggcggagttg tcgattcgcc gccatgtgct ggcctatatg ctgtcggcgg tgctggtgct
     2581 gtttggcatg attgcctatc aaaaaatcgg cgtagaccgt tacccgaata tcgaacagcc
     2641 ggtgatttcg attgccacca cgctgactgg cgccacgccc gaggtgatgg acagcagcgt
     2701 gacgcagatc atcgaagcgg cggtgaacag tgtgccgggc atcgacagca tcgaatccac
     2761 ctcgcaaccc ggccgctcga cggtgaaagt caccttcaac ctcgacaaga acatcgacgt
     2821 ggcgttcaac gaggtgcaag tcaaggtcaa ccaggcgcag cgccggctgc cggatgatgt
     2881 ggacatgccg gtggtgtcca agaccgacgc caacggcagc ccgattgtct ggctggcgct
     2941 gactggcgac cgcaccgaaa agcagctgta tacctacgcc aacaccgtgg tgaaaaaaca
     3001 gctggaaacc gtcgatggcg tcggcgaggt ggtgctgcgc gggcgcggcg cgcgggtgat
     3061 ccgcgtggag ctggacccgg tcaaactggc cggctacaac ctgacgccgg ccgaggtgaa
     3121 atccgccttt ggccgcgaac acctgcaaca gcccggcggc acggtggtca acggcccggc
     3181 tgagttgctg gtgaatctgg atctggaatt caaatcggtg cgggcgctgc aagacctggt
     3241 ggtggcctgg cgcggcggcg gcgccatccg cctggccgac gtggcgcggg tgatcgacgg
     3301 cgaaggcgat ttgcgcagcc tgacccgctt taacggcaaa cccgccgtgc tggtgggcat
     3361 tgtgcgggtg gccaacagca ataccgtggc gctcagccag gcggtgatcg agcgggtaga
     3421 tgggcaaatc cgcccagcgc tgccgcccgg cattgagctg actttggcgg ccaacgacgc
     3481 cagctttgtg gtggagatga ttggcgcgct gaaagaccat ttattcgaag gcacgctgct
     3541 ggccgggctg attgtctggc tgtttttgcg cagcttgcgc tcgaccttga ttatctcgct
     3601 ggccattccg gtgtcgctgc tgggcgcggt ggcggtgatg tattttctgg gttacacctt
     3661 caacaccgtg acactcctgg ggcttttgct gctgattggc gtggtggtgg acgacgctat
     3721 cgtggtgctg gaaaacatcc accgcctgcg tgagcaaggc cacgacaacc cgattgaagc
     3781 ggcgattctg ggcagccagc aagtggtgtt tgcagtgatt gccgccacgc tgtcgctggt
     3841 ggcgattttt gtgccggtgg cgtttttggg cggcattgtc gggcgcttgt tcaattcgtt
     3901 tgccctggtg gtggcctgcg gcgtgctggt gtccttgttt gtgtcggtga cgctgacgcc
     3961 gatgctgtgc agccgctttc tgaaagtgga gaaacaacac ggccgggtat acgtttggct
     4021 ggagcgcggc tttcaggcca tggaaaacgg ctaccggcgg gtgctggact ggaccttgcg
     4081 ccaccgtggc aaagtgctgg gctttgccct gttgagcgtg accgccagcg tggcggtgtt
     4141 catgctgctg aagtcagaat ttttgccgga agaagacgaa agccgctttc tggtgaacat
     4201 caaaaccccg attggcgcat ctatccacta caccaaccaa aaaagccgcg aagtcgaagc
     4261 catcctgctg cgtcacggcg aggtgcgcaa tctgctgacg attgttggtg gtttcagcgg
     4321 cacgcaagtg aatcaagcca cctttgtggt gcgtttgacc gcccggcaca cccgcaccgt
     4381 acgccagggc gagctgctgg ccaaggtgcg caaagagctg ggccatgtac cgggcatccg
     4441 cgcctcggcg tttccgattc cccgggtggg ggaaagtcgc ggcggcaagc tacagtttgc
     4501 cgtgatcggg ccgaatttaa ctgacgtggc caaacatgcc gatgcgctga tgcagcggct
     4561 gggcagccaa cccggtattg gccggctgga catcgacctg gatctggaac aacctcagtt
     4621 gtcattcgac atcgaccgcg cagccgccgc gcgggtgggg gtgtctaccg aaagcatcgc
     4681 caccgccttg aacctgctga ccgcaggcag taatgtggcg aaattttccg atggcgacgg
     4741 agagcgctat gatgtgcggg tgaaggccgc agacatggcg atcagccggg ccgaagacct
     4801 caacgccatt tatctgcgca gccagaatgg cgagctggtg cgactggacg ccgtggtgcg
     4861 ggtcaagcgc accctgggcc ccacgctgat tgcccggcag aacctgcaat acgcggccaa
     4921 tctgaaaggc tcgcccaccc tggcgctggc cgaagcggtg gccgaggtgc aggcgacagc
     4981 gcgtgacata ttgccccctg gttatcgggt agagatgcag ggtgaggcgc gcgagctggc
     5041 gcgcggaggg gggcaggcgg tgtttatctt tggcctggcc tcgttgttgc tgtatatgat
     5101 tctggccagc cagttcaact cgtttgccca gccagccatc atcatgctgg ccgagccact
     5161 ggcggtggtg ggcggcctgc tgttgctgtg gttgtccggg catactttga acgtgtactc
     5221 gatgatcggg ctgattttgc tgattggcct ggtggcaaag aattcgatcc tgctggtgga
     5281 cgtcaccaac cagtttcggg cagccggtca tgctgtcgat gatgccttgc gggccagttg
     5341 ccccttgcgg ctgcgcccgg tgctgatgac ttcgctgacg gtgatcctgg ccatgctgcc
     5401 agcggcactg ggcctgggcg ccggcgccga aaccaatggg ccgctggccg tggcagtgat
     5461 tggtggcatg atcagttcca cgctgctcac cctggcggtg gtgccagcag cgtattcttt
     5521 gctggaaaac cgtcgacagg cacaataatc tagtagaaat acctaaccga cttgccagcc
     5581 cggtcaggaa tggcgatgat gatgccattc tcgggacgcc cgagtgagga ttgcgcgcaa
     5641 tgagtgaata cgaagaagag atgaatcaat cgccgttcgg cctgaccatg acggaaaccg
     5701 aagcgggtga attgcgcctg atgttcgagc cggacgattt ccgcttcaag attgatgcca
     5761 cggccttgcg ccacatggcc atcgagaagg gctatggcga gctggatttc gacgaaaacg
     5821 ccctggccgt ggcggctcag cacgtttgca aaaacgaggc ctttgtctcc accattgccc
     5881 agcgccgcaa tgccgaatat cgggtgtatg tgtcgtccga ccgcctggtg gcgcagctgc
     5941 aggtgacccc atcttacggt ggcacgccga tttcggtgga tgctgcccgc aacgagctgg
     6001 ccaaagcacg ggtggtgttc ggctttgacg aagcggcgtt ccagtcggca ctgaccaata
     6061 agctgggcga acccgtgacg gtggccaagg gcgtggcgcc gattcctggc cgcgacaccc
     6121 gctttgaacc gctggtggaa gtcaaccgcg agcgcgtgcc gcgcgccaat gaagacggcc
     6181 gggtcaactg gcgcgacctg ggcgacatcc ccatggtgtc gccgggcgat gtgctgatgc
     6241 gtcgtcatcc gcccgagcaa ggcaagtctg gccgcgatgt gttcgacaat atcctgcaag
     6301 cgccggacgg caagaatctg gactacccct accggctgga aggcgccgag cgcgacgcca
     6361 acgaccccga cctgctgcgc gccaccagca ccggccagcc ggtcttgctg cgcgattcgg
     6421 tgaaagtcga accggtgatc aagactccgg gggtgaacgt cagcaccggt aacatcacct
     6481 ttgtcggcag tctggtgatc aacggtgatg tacaaagcgg catgcatgtg aaggtggatg
     6541 gtgacgtcac cgtgcacggc acggtggaag ccgccatcat tgaagctggc ggcagcatca
     6601 ccgtcaacgg cggcatcgtc ggccagaacg cccaacaacg cgaatccaat gccaacggcg
     6661 aacaggccaa gctgcacgcc aaggccaatg tgcgggccaa gtatgccgaa aacgcgctga
     6721 tcatcgccga gcaatcggtg tatatcgacg aagcggtgat cgaatgcgac atcaccgcgc
     6781 tgaaccgggt ggaaatcggc gtgcgcggcc ggcgcaaggg ctacatcatg ggcgggcgcg
     6841 t
//
LOCUS       DAOPYX010000155         6826 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_13581_length_6826_cov_10.504209, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000155 DAOPYX010000000
VERSION     DAOPYX010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 6826)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6826)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6826
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13581_length_6826_cov_10.504209"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(32..2053)
                     /gene="rsxC"
                     /locus_tag="PKN55_08820"
     CDS             complement(32..2053)
                     /gene="rsxC"
                     /locus_tag="PKN55_08820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012696700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HNX71387.1"
                     /translation="MTPTLTPFHGGVHPPEHKQQSSEAPIRAAGLPPLLRLSLAQSMG
                     APARPCVSVGQRVLKGQRLADADGAMSVAVHAPTSGVVRAIGPLPLAHPSGLNGPGIE
                     LEPDGDERWIELTPLDWRHADPAQVLDTLQARGVAGLGGALFPTHLKLARRGLDTLVV
                     NGAECEPYITCDDRLMRERASGIVAGIALAAQLTEARQVLIGIEDNKPQAIAAMRAAC
                     AGSGFDVVVIPTLYPSGGAKQLIHLLTGKAVPAGRRSTEFGVQCLNVATLYTAARALE
                     HGEPAIQRLVTLTGAVARPGNVEALLGTPADWLLAEAGRQADSDQIIMGGPMMGFALP
                     DASAGLTQASNCLIAASPTLFPPRPPAMPCIRCGDCAQVCPAELQPMDLHWFAKARQF
                     DKARDAHLFDCIECGACSYVCPSQIPLVDYYRFAKSELWASDRDKKAAERARQRHEFR
                     QFRLERDKTEKAERLAQRSAALASQAAASTATAADAPSTDDAGKRAAIEAAMARAAAR
                     QAEQTAAKADSAASAAAPSDDAKRAAIEAAMARAAARKAEQAAAKADTAAPTAAPSDD
                     AKRAAIEAAMARAAARKAEQAAAKADTAAPAAAPSDDAKRAAIEAAMARAAARKAEQA
                     AAKADSAAPTAAPSDDAKRAAIEAAMARAAARRAEQAAKAATPPNSEPQ"
     gene            complement(2050..2625)
                     /gene="rsxB"
                     /locus_tag="PKN55_08825"
     CDS             complement(2050..2625)
                     /gene="rsxB"
                     /locus_tag="PKN55_08825"
                     /inference="COORDINATES: protein motif:HMM:TIGR01944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HNX71388.1"
                     /translation="MSGALLTLAVLVGLGLLLGALLGFAAVRFKVDGDPLVDKIDAIL
                     PQTQCGQCGHPGCRPYATAIAAGEADINQCPPGGEDGIRKLAELLGREFKPFAEGAAQ
                     PKPKAVARIDESSCIGCTLCIQACPVDAIVGASKLMHTVIASECTGCELCLPPCPVDC
                     ISMQPLADTPRTWKWRYPVVMMAAAPQDPHP"
     gene            complement(2622..3209)
                     /gene="rsxA"
                     /locus_tag="PKN55_08830"
     CDS             complement(2622..3209)
                     /gene="rsxA"
                     /locus_tag="PKN55_08830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012696702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HNX71389.1"
                     /translation="MTLDFSHYLLIIVGAVLVNNVVLVRILGLCPFMGVSKKLDAAIG
                     MGAATAFVLTLASGASHLLNLLLLAAGLEYLRTIAFIVVIAAIVQFTEMVIHKTSPVL
                     YQALGIYLPLITTNCAVLGVPLLNAQQGHSFVEALLFGLGSALGFSLVLVLFAALRER
                     LEGADVPAPFRGAPIALVTAGLMSLAFAGFAGLTR"
     gene            3493..4869
                     /locus_tag="PKN55_08835"
     CDS             3493..4869
                     /locus_tag="PKN55_08835"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNX71390.1"
                     /translation="MPPNAASSDELADWHMHSVLVVDDEPGMVNFLRRALAARCGVLD
                     SAGDVASAAALLQRQHYDLIILDISLPGGRSGTQWLHELRGEGFAGDVVLMTAYADLD
                     TAIDALRAGAADFLLKPFSLAQLLNAVKRCFERSRLQQENYVLRREVTAQGGLEGLVG
                     QSEAMRLVCERLKRIAPTPSTVLLLGESGTGKEVAARALHRMSPRADGPFVPVNCAAI
                     AADLIESELFGHVKGAYSGASSSREGLFYYARGGTLFLDEIGELPLAAQAKLLRALEE
                     RRVRPVGSEQEIPVNARVVAATNRDLAQEVAAGRFRQDLYYRLQVMEIELPALRERPE
                     DIPELINHFTRQLAGQLGVPPVHPDAASLRRLQGYDWPGNVRELRNLVERSLIFGYYD
                     LKPEPASAAAEAGAALPAQPEVLLEAVEKRHILAVLNACGGNKSEAARQLGVSRKTLD
                     RKCNAWGV"
     gene            4848..>6826
                     /locus_tag="PKN55_08840"
     CDS             4848..>6826
                     /locus_tag="PKN55_08840"
                     /inference="COORDINATES: protein motif:HMM:NF028510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HNX71391.1"
                     /translation="MQRLGGVSRISGGNWRAPSIRLRLLALALAPLAMVLPVLVAILA
                     SWGSEYFDRLMVTKVRSDLAVAHGYFERVQAGVGHAVQALAGSERLARARRAHDAGAL
                     NTLLADARTQLQLDFLLLLDAHGCLPDARPALCLADWPVERAALAGQGRTELDVFSPT
                     LLAQLSPGLAGQAAMALRPNDKAAPTTRSQENRGLLIHTAAPVLDADGRLQGVLAGGV
                     LLNRNLEFIDHLNAVVYPRDALPFGSQGTATLFLGDVRVATNVRLFAGERAIGTRVSA
                     TVNQAVLQEGGVWLNRAFVVQDWYVSGYMPLMDSHQRRVGMLYVGFLEEPLVRAKRAA
                     LGLVVALFIVVMGGASLLAVWWARGIYLPIQRMQATMRAWGAGKLSARVGPLTPLDEM
                     AELARHFDRLLDQLQSQTEALQQWGNALDGEVARRTQDLSQALADLRSAQHQLVRQEK
                     LAAMGQLTAGVAHEINNPVAVIQGNLDILIDVLGPQGCEPVRDEIRLIREQIHRIRMI
                     VAKLLQFSRPTEYVGYLEPIALDALVQDGLLLVGHLLRRTSIAVTQQLDSSRLVVGNK
                     GELQQVLINLLVNAIQAMPEGGVLTLSSEDWDEAGMPIGVRLSVSDTGPGIPPAHLDK
                     LFQPFFTADKADGNGLGLWVSQGIVERYGGNLTAA"
BASE COUNT          984 a         2189 c         2480 g         1173 t
ORIGIN      
        1 cttttgggtt gggcaagaaa aggagaagac atcattgcgg ttcactgttc gggggagtgg
       61 cggccttggc cgcctgttcg gcgcgacgcg cggcggcgcg ggccatcgcc gcttcaatcg
      121 cggcgcgctt ggcgtcgtca cttggcgcag cagtgggcgc ggcgctgtct gccttggcgg
      181 cggcttgttc ggctttgcgg gcggcggcgc gggccatcgc cgcttcaatc gcagcgcgtt
      241 tggcgtcgtc gctcggcgca gcagcgggcg cggcagtgtc tgccttggcg gcggcttgtt
      301 cggctttgcg ggcggccgcg cgggccatgg cggcttcgat ggcggcgcgt ttggcgtcat
      361 cacttggcgc ggcagtgggc gcggcagtgt ctgccttggc ggcggcttgc tcggctttgc
      421 gggcggcggc gcgggccatg gcggcttcga tggcggcgcg tttggcgtcg tcgcttggcg
      481 ccgcagcgga cgcggcgctg tctgccttgg cggcggtttg ctcggcttga cgggcggcgg
      541 cgcgcgccat cgctgcttca atggccgcgc gtttgccggc gtcgtcggtg ctgggggcgt
      601 cggcggcggt ggctgtgctg gcggcggctt ggctggccag ggcggcgctg cgctgggcca
      661 ggcgttcggc tttttcggtt ttgtcgcgct ccaggcggaa ctggcggaat tcatggcgct
      721 ggcgggcgcg ctcggcggct tttttgtcgc ggtcgctggc ccacagctcg cttttggcaa
      781 agcggtaata gtccaccaag ggaatctggc tggggcagac atagctgcac gcgccgcatt
      841 cgatgcagtc aaacaaatgg gcgtcgcgcg ccttgtcaaa ctggcgcgcc ttggcgaacc
      901 agtgcaagtc catcggctgc aattcggccg ggcacacctg ggcgcagtcg ccgcagcgga
      961 tgcacggcat ggccggcggg cgcggcggaa acagcgtcgg gctggcggca atcaggcagt
     1021 tgctggcctg ggtgaggccg gcgctggcgt cgggcagggc aaagcccatc atcggcccgc
     1081 ccatgatgat ctggtcgctg tccgcctggc ggccggcttc ggccagcagc cagtcggccg
     1141 gcgtgcccag cagcgcttcg acattgcccg ggcgcgccac tgcgccggtc agcgtcacca
     1201 gccgctggat ggccggctcg ccatgctcca gcgcgcgggc ggcggtgtag agggtggcca
     1261 cgttcaggca ttgcacgcca aattcggtcg agcgccgccc ggccggcacc gccttgccgg
     1321 tcagcaggtg gatcagctgc ttggcgccgc cgctggggta gagggtgggg atgaccacca
     1381 cgtcaaaccc gctgccggcg caggcggcgc gcatggcggc aatcgcttgt ggtttattgt
     1441 cttcaatgcc aatcagcacc tggcgcgcct cggtcagttg ggcggccagg gcgatgccgg
     1501 cgacgatgcc gctggcgcgt tcgcgcatca gccggtcgtc gcaggtgata tagggctcgc
     1561 actcggcgcc gttgaccacc agggtgtcca gcccgcgtcg ggccagcttg aggtgggtgg
     1621 ggaacagcgc gccgcccagg ccggccacgc cgcgcgcttg cagggtgtcc agcacctgcg
     1681 ccgggtcggc gtggcgccag tccagcgggg tgagttcgat ccagcgctcg tcgccgtccg
     1741 gctccagctc gatgccgggg ccgttcagcc cggaggggtg ggccagcggc agcgggccga
     1801 tggcgcgcac cacgcccgag gtgggcgcgt gcaccgccac cgacatggcg ccgtcggcgt
     1861 cggccaggcg ctggcccttg agcacgcgct ggccgacgct gacgcagggc cgcgcgggcg
     1921 cgcccatgct ttgcgccagc gacaggcgca gcagcggcgg caggccggcg gcgcggattg
     1981 gcgcttcgct ggactgttgt ttgtgttcgg gcggatgcac gccgccatga aacggcgtca
     2041 gcgtgggggt catgggtgcg ggtcctgtgg ggcggcggcc atcatcacca ccgggtagcg
     2101 ccatttccag gtgcgtgggg tgtcggccag cggctgcatg ctgatgcagt ccaccggaca
     2161 gggcggcagg cacagctcac agccggtgca ttcgctggcg atgacggtgt gcatcagctt
     2221 ggaggcgccg acaatggcgt ccaccgggca ggcctggatg cacagcgtgc agccgatgca
     2281 cgagctttcg tcgatgcgcg ccaccgcctt gggtttgggc tgcgccgcgc cttcggcaaa
     2341 cggcttgaat tcgcgcccga gcagctcggc cagcttgcgg atgccgtcct cgccgccggg
     2401 cgggcattgg ttgatgtcgg cttcgccagc ggcaatcgcc gtggcgtagg gccgacagcc
     2461 ggggtggccg cattggccgc attgggtttg cggcaggatg gcgtcgattt tgtccaccag
     2521 cgggtcgccg tccactttaa agcgcacggc ggcaaagccc agtagcgcgc ccagcagcag
     2581 gcccaggccc accagcacgg ccagcgtcag cagcgcgccg ctcatcgcgt cagcccggca
     2641 aagccggcaa acgccaggct catcagcccg gcggtcacca gcgcaatcgg cgcgccgcga
     2701 aacggcgccg gcacatcagc gccttccagc cgctcgcgca gcgcggcaaa cagcaccagc
     2761 accagcgaaa agcccagcgc gctgcccagg ccgaacagca gcgcctcgac aaagctgtgg
     2821 ccttgctggg cgttgagcag cggcacgccc aataccgcgc agttggtggt gatcaagggc
     2881 aggtagatgc ccagcgcctg atacagcacc gggctggttt tgtggatgac catttcggtg
     2941 aactgcacga tggcggcaat caccacaata aaggcaatgg tgcgcaggta ttccagcccg
     3001 gcggccagca acagcaggtt gagcaggtgg ctggcgcccg aggccagggt gagcacaaag
     3061 gcggtggcgg cgcccatgcc gatggcggcg tcgagttttt tcgacacgcc catgaacggg
     3121 cacaaaccaa gaatgcgcac cagcaccaca ttgttgacca gcaccgcgcc aacaataatc
     3181 aggagatagt ggctgaaatc cagcgtcatg aaaaatgtcc tgcgccgccc tgttggtaca
     3241 gcggcggcgc gtccggccgg ggccgggcag tgttcggggt gaggcgggcg gcctgcgccg
     3301 tccgtcggat gcgccggcag acatgccgga cgggcaatat tatccgacgg catcggtcgg
     3361 gtttcaaccg tgatgcagtc atccccccga cgcggcccgg catgtgttgg ccatcgcggc
     3421 ttggccgctt acctttgcta ttattgtggt ttttggtctg acccccgcgc ccgggggctt
     3481 ccggagcgcc tcatgccccc caacgcagct tcttctgacg aactggccga ctggcacatg
     3541 cactcggtgc tggtggttga cgacgaaccc ggcatggtca attttttgcg ccgcgccctg
     3601 gccgcccgct gcggcgtgct ggacagcgcc ggcgacgtgg cctcggccgc cgcgctattg
     3661 cagcgccagc attacgatct gatcattctg gatatctccc tgcccggcgg gcgcagcggc
     3721 acgcaatggc tgcacgagct gcgcggcgag ggctttgccg gcgatgtggt gctgatgacc
     3781 gcctacgccg acctggacac cgccatcgac gccctgcgcg cgggcgcggc ggatttcttg
     3841 ctcaagccgt tttcgctggc gcagctgctg aatgcggtca aacgttgctt tgagcgctcg
     3901 cgcctgcaac aggaaaacta cgtgctgcgc cgcgaagtca ccgcccaggg cgggctggaa
     3961 gggctggttg gccagtccga ggcgatgcgc ctggtgtgcg agcggctcaa gcgcattgcg
     4021 ccgacgccgt ccaccgtgct gctgctgggt gaatccggca ccggcaagga ggtggcggcg
     4081 cgcgcgctgc accgcatgag cccgcgcgcc gatgggccgt ttgtgccggt gaactgcgcg
     4141 gcgattgccg cagatttgat cgagtccgag ctgtttggcc atgtcaaagg cgcgtattcc
     4201 ggcgccagca gcagccggga agggctgttt tactacgcgc gcggcggcac gctgtttctg
     4261 gatgaaatcg gcgaattgcc gctggcggcg caggccaagc tgttgcgcgc gctggaagag
     4321 cgccgggtgc ggccggtggg ctccgagcag gaaatcccgg tcaacgcccg cgtggtggcg
     4381 gccaccaacc gcgatctggc gcaagaagtc gccgccgggc gttttcgcca ggatttgtat
     4441 taccgcctgc aagtgatgga aatcgaactg ccggcgctgc gcgagcgccc ggaagacatc
     4501 ccggaactga tcaaccactt cacccgccaa ctggccggcc agttgggcgt gccgccggtt
     4561 caccctgacg cggcgagctt gcgccggctg caaggctacg actggccggg caatgtgcgc
     4621 gaattgcgca atctggtcga gcgctcgctg atttttggct attacgacct caagcccgag
     4681 ccggccagcg ccgccgccga ggctggcgcc gcgctgccgg cgcagccgga ggtgttgctg
     4741 gaggcggtgg aaaaacgcca tatcctggcc gtgctcaacg cctgcggcgg caataagtcc
     4801 gaggcggccc ggcagttggg cgtgtcgcgc aaaacgctgg accgcaagtg caacgcctgg
     4861 ggggtgtaag ccggatcagc ggcggcaact ggcgcgcgcc gtcgatccgc ctgcgcctgt
     4921 tggcgctggc gctggcgccg ctggccatgg tgctgccggt gctggtggcc attctggctt
     4981 cgtggggcag tgaatatttt gaccggctga tggtcaccaa ggtgcgcagc gatctggcgg
     5041 tggcgcatgg ctattttgag cgggtgcaag ctggcgtggg ccacgccgtg caggcgctgg
     5101 ccggctctga gcggctggcg cgcgcgcgcc gcgcccacga cgccggcgcg ctcaacaccc
     5161 tgctggccga cgcgcgcacc cagttgcagc tggatttttt gctgctgctg gacgcccacg
     5221 gctgcctgcc ggatgcccgc ccggctttgt gtctggcgga ctggccggtg gagcgtgccg
     5281 ccctggccgg ccaggggcgc accgagctgg atgtgttctc gcccaccttg ctggcgcaat
     5341 tgtcccccgg cctggccggg caggcggcga tggcgctgcg cccgaatgac aaggcggcgc
     5401 ccaccacgcg cagccaggaa aatcgcggct tgctgattca caccgccgca ccggtgctgg
     5461 acgccgacgg ccgcttgcag ggcgtgctgg ccggcggcgt gctgctcaac cgcaatctgg
     5521 aattcatcga tcacctcaac gccgtggtgt atccgcgcga cgccttgccg tttggcagcc
     5581 agggcaccgc cacgctgttt ttgggcgatg tgcgggtggc caccaatgtg cggctgtttg
     5641 ccggcgagcg cgccatcggc acccgcgtgt cggccaccgt caatcaggcg gtgttgcagg
     5701 aaggcggggt gtggctgaat cgggcgtttg tggtgcagga ctggtatgtg tctggttata
     5761 tgcccttgat ggacagccat cagcgccggg tgggcatgtt gtacgtgggc tttctggaag
     5821 aaccgctggt gcgcgccaag cgtgcggcgc tggggctggt ggtggccttg ttcattgtgg
     5881 tgatgggcgg cgccagcctg ctggcggtgt ggtgggcgcg cggcatctac ctgcccatcc
     5941 agcgcatgca ggccaccatg cgcgcctggg gcgccggcaa gctcagcgcc cgcgtcggcc
     6001 cgctcacccc gctggacgaa atggccgaac tggcccgcca cttcgaccgg ctgctggatc
     6061 agctgcaatc gcaaaccgag gcgctgcaac aatggggcaa tgcgctggat ggcgaggtgg
     6121 cgcggcgcac ccaggatttg tcgcaggcgc tggccgattt gcgctcggcc cagcatcagc
     6181 tggtgcgcca ggaaaaactc gccgccatgg gccagctgac cgctggcgtg gcgcacgaaa
     6241 tcaataaccc ggtggcagtg atccagggca atctggacat cctgatcgac gtgctcggcc
     6301 cacagggctg cgagccggtg cgcgacgaaa tccgcctgat ccgcgagcag attcaccgca
     6361 tacgcatgat tgtcgccaag ctcttgcagt tttcccggcc aactgaatat gtcggctatc
     6421 tggagccgat tgcgctggac gcgctggtgc aggacggcct gctcttggtg ggccatctgc
     6481 tgcgccgcac ctcgattgcc gtcacccagc agctggattc gtcgcgcctg gtggtgggca
     6541 ataagggcga gctgcaacag gtgttgatca atctgctggt caacgccatt caagccatgc
     6601 cggaaggcgg ggtgttgacg ctgtccagcg aggattggga cgaggccggc atgcccatcg
     6661 gtgtgcgcct gtcggtcagc gacaccggcc ccggcatccc gcccgcgcat ctggacaagc
     6721 tgtttcagcc gtttttcacc gcggacaagg cggacggcaa tggcctgggc ctgtgggtga
     6781 gccagggcat tgtcgagcgc tacggcggca acctcaccgc cgccaa
//
LOCUS       DAOPYX010000156         6805 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_13635_length_6805_cov_11.553333, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000156 DAOPYX010000000
VERSION     DAOPYX010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 6805)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6805)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6805
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13635_length_6805_cov_11.553333"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..557)
                     /gene="paaY"
                     /locus_tag="PKN55_08845"
     CDS             complement(<1..557)
                     /gene="paaY"
                     /locus_tag="PKN55_08845"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019923191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation protein PaaY"
                     /protein_id="HNX71392.1"
                     /translation="MSELTVYAIDGLTPVVHPDAYVHPSAVLIGDVIIGARCYIGPCA
                     CLRGDFGRLILHDGANVQDTCVLHGFPGADTVIEEDGHIGHGAVLHGCRIGRNALVGM
                     NAVVMDEAVVGESAIVAASAFVKAGMEIPPGMLAAGVPAKVVRPLSELERQWKRDGTA
                     CYHDLARRSLASLTATTPLREMEANR"
     gene            638..1429
                     /locus_tag="PKN55_08850"
     CDS             638..1429
                     /locus_tag="PKN55_08850"
                     /inference="COORDINATES: protein motif:HMM:NF013757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HNX71393.1"
                     /translation="MHDDLEGKHGVQSLEVGMSLLKAMVAGQQAMMLKDIAAAADMPA
                     SKAHRYLVSLIRSGLVEQDPLSSRYTLGPFALNLGLVALGRLDRVRLGLSAIADLRDA
                     INETTALAVWGGQGPVIVRWERPRRPITVNVITGATLSLLASAAGRVFAAWLPPAQIE
                     PLVQAELDSGKCRANVQTLDEARAVLAEVRARGLAAISGHYMVRGVEAMAAPVFNFKQ
                     DITLGLVVVGVEGSLDMREDSPTVTALREAAHALSLRLGYAPPAA"
     gene            1758..2681
                     /locus_tag="PKN55_08855"
     CDS             1758..2681
                     /locus_tag="PKN55_08855"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HNX71394.1"
                     /translation="MDDPHCIGRLAGRRCVMLAQFLQFLFSGVTVGATYALAALGFTL
                     IYNASHVINFAQGEFIMLGGMLAVFFTQAGLPLPVALLLAVLLPALVGVLVEKLAIEP
                     VKGAPTVSLIIITIGASLVIRGLTQVWLGKGTHSLPAFSGDAPLALLGATLLPQSLWV
                     LGVTALVVAALWGFFNHTLLGKAMLATSFNSLAAQLVGVHTGRVLFLSFAMSGALGAL
                     GGVLVTPITLTSYDVGIMLGLKGFVAAVVGGLGNGLGAVLGGLLVGVLEALGAGYVSS
                     AYKDAIPFALILLMLFFMPQGLLGARNTERV"
     gene            2684..3619
                     /locus_tag="PKN55_08860"
     CDS             2684..3619
                     /locus_tag="PKN55_08860"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HNX71395.1"
                     /translation="MTTSRYLPLGALAVGLLVLPWLLPNSFYLDLAIRMAINAIIVLG
                     LNLLIGFAGQISLGHAGFLGLGAYASAVLPTHFGWPPLAAMLAGMALTGVLAALVARP
                     IFRLKGHYLAMATLGLGIILNIVVRNEAQWTGGPDGMPVLPFEVAGLTLSRDWHWWWL
                     VSALLLLSVWMAQNLMDSPFGRALRALHGSEVASRGVGVDVAGYKVAIFVLSAMFASL
                     MGSITAHYVGFVTPNVADFFHSIELVTMVVIGGMASVYGSLLGAVLLTALPQLLATFE
                     GWETVVFGAILMLCMIFLPKGLAPTLAARFGKGGQ"
     gene            3621..4382
                     /locus_tag="PKN55_08865"
     CDS             3621..4382
                     /locus_tag="PKN55_08865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011135057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNX71396.1"
                     /translation="MALLEVSELSIQFGGVKAVQAVSFSIDAGMIYAVIGPNGAGKTT
                     LFNLITGVYTPTAGSIRLDGQPVHGLTPDRLAQRGVARTFQNLQVCMNMLAIENVMVG
                     AHLRLDRSLFKAALRWPALRRADAALRDEAAELMRFVGLAQYLTARADSMPYGALKRL
                     EIARALAMRPRLLFLDEPAAGLNPKETQEVDALVRRIADAGVTVVLVEHDMKMVMNLS
                     ERILVLDYGKKLAEGNRDEIRRNPDVIAAYLGAHA"
     gene            4397..5701
                     /locus_tag="PKN55_08870"
     CDS             4397..5701
                     /locus_tag="PKN55_08870"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNX71397.1"
                     /translation="MHSALHIIQDEHRNLWRIAITLDRLGEDIAGGGALEPAFLLALF
                     DYLEHFVDAAHHRKEDEHLFACLRQRDASAIPLLDALQAEHREAPARVQALRQQLADC
                     LHGQGQASALAQGLRGYADGLKSHIRREEQQVFPLAEQALSAADWDAIQQAFSSHQDP
                     LFGDAASAESRELFHRVASLAPESVGLGGHSGGELASHQDWLLEVDALDSSYGRIQAL
                     SGISLKVRRGETVALVGANGAGKTTLLRALSGVQPARAGQLRFAGEDIRRLRADLRVR
                     RGICQSPEGRQVFGPLSIEDNLRLGGYTQPRAQADSDLEQVYAMFPVLKEKRRLPAGT
                     LSGGQQQMLAIGRALMGRPKLLLLDEPSMGLAPLLVEEVFNVVKTLKAQGMTLILAEQ
                     NAFSALALADRGYVLETGRITLSGGGRELIDNEQVRAAYLGM"
     gene            complement(5811..6356)
                     /locus_tag="PKN55_08875"
     CDS             complement(5811..6356)
                     /locus_tag="PKN55_08875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008952354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HNX71398.1"
                     /translation="MSAELPAAGQPVKVWDGFIRLFHWSLVAIVALNLFVLEEGDVAH
                     RWLGYFAAGAVLARVLWGFVGSRHARFADWFPTPARLRAHVCAMRRGEADNHLGHNPL
                     AGLMILTLLSVILGLAVSGWLMGTDAYFGVEWVEELHEALSAGLQGLVAVHVLAALLM
                     GRKLRQPLIRAMITGYKRSPG"
     gene            complement(6459..6716)
                     /locus_tag="PKN55_08880"
     CDS             complement(6459..6716)
                     /locus_tag="PKN55_08880"
                     /inference="COORDINATES: protein motif:HMM:NF025051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HNX71399.1"
                     /translation="MQRIALAAVLSIASLSAFAGQSCTDAPKSSWMSKEQITAQVKGM
                     GYTIKKLGVEDNCYEVKGLDKDGKKVEAYFNPVDGKLVKTK"
BASE COUNT         1116 a         2146 c         2266 g         1277 t
ORIGIN      
        1 cggttggctt ccatttcacg cagtggcgtc gtagcggtca ggctggccag gctgcggcgc
       61 gccaggtcgt ggtagcaggc ggtgccgtcg cgcttccatt gccgctccag ctcgctgagc
      121 gggcgcacca ccttggcggg cacgccggcg gccagcatgc cgggcgggat ctccatgccg
      181 gccttgacga aggccgaggc ggcgacaatc gccgattcgc ccaccaccgc ttcgtccatc
      241 accacggcgt tcatgcccac cagcgcgttg cggccgatgc ggcagccgtg cagcaccgcg
      301 ccatggccga tgtggccgtc ctcttcgatc actgtgtccg cccccggaaa accatgcagc
      361 acgcaggtgt cctgcacatt ggcgccgtcg tgcaggatca gccggccaaa gtcgccgcgc
      421 aggcaggcgc aggggccgat atagcagcgc gcgccgatga tgacatcgcc aatcagcacg
      481 gcggaagggt ggacgtaggc gtccgggtgg accaccgggg tcaggccgtc gatggcgtaa
      541 accgtgagtt ctgacattgc tcgcgctcct gtcgcatgat ttaattctgt attacaaaat
      601 cagtttctta ttacagaata ccgcagggag cacagggatg catgacgatc tggaaggcaa
      661 acatggcgtg cagtcgctgg aggtgggcat gagcctgctc aaggcgatgg tggccgggca
      721 gcaggcgatg atgctcaagg acatcgccgc tgcggccgac atgccggcgt ccaaggcgca
      781 ccgttatctg gtcagcctga ttcgcagcgg gctggtcgag caagacccgt tatcgtcgcg
      841 ctacacgctg gggccgtttg cgctcaatct gggcctggtg gcgctgggcc ggctggaccg
      901 ggtgcggctg gggctgagcg cgattgccga tttgcgcgac gccatcaacg aaaccacggc
      961 gctggcggtg tggggcggcc aggggccggt gattgtgcgc tgggagcgcc cgcgccggcc
     1021 gatcaccgtc aatgtcatca ccggggccac gctcagcctg ctggcctcgg ccgctggccg
     1081 ggtgtttgcc gcctggctgc cgccggcgca gatcgagccc ttggtgcagg ccgagctgga
     1141 cagcggcaag tgccgcgcca atgtgcagac gctggacgag gcgcgcgccg tgctggccga
     1201 ggtgcgcgcg cgcgggctgg cggcgatttc cgggcattac atggtgcgcg gcgtcgaggc
     1261 catggcggcg ccggtgttca acttcaagca ggacatcacc ctcgggctgg tggtggtcgg
     1321 cgtggaaggc agcctggaca tgcgcgaaga cagccccacg gtgaccgcgc tgcgcgaggc
     1381 ggcgcacgcc ttgtcgttgc ggctgggcta tgcgccgcca gccgcttgag cgccttgcgc
     1441 cgatctgcgc caccgcccgc cagggcggtt tttttgcgcc gtcatccgct gtgcaccgca
     1501 aaaaaagata cataaaaatt ttcttgtagg caagtttgcg tatcgctttt tattttgatg
     1561 gcactgcaca tgcaagcaaa agtctatgaa aattttgcca tccctccctg ttttgtctgg
     1621 ttaagtgata cataaatcta gattgacaga atttattgtg tcatttctaa tgtgcgtaac
     1681 ggattacaca atacagaatc ttcgcgccac ccggacgcgc agaaccgttg cccacctgac
     1741 ccaaaccatg aggagacatg gatgaccccc actgcattgg ccgcctggcc ggcaggaggt
     1801 gcgtgatgct ggcgcaattc ctgcaatttt tgttttccgg cgtcaccgtc ggcgccacct
     1861 acgcgctggc ggcgctgggc tttaccctga tttacaacgc cagccatgtg atcaacttcg
     1921 cccagggcga attcatcatg ctgggcggca tgctggcggt gtttttcacc caggccggcc
     1981 tgcccttgcc ggtggccttg ttgctggcgg tgctgttgcc ggcgctggtg ggggtgttgg
     2041 tggaaaaact ggccatcgag ccggtgaagg gcgcgcccac cgtgtcgctg atcatcatca
     2101 ccattggcgc ctcgctggtg attcgcgggc tgacccaggt ctggctgggc aagggcacgc
     2161 acagcctgcc ggcgttttct ggcgatgcgc cgctggcgct gctgggcgcc accttgctgc
     2221 cgcaaagcct gtgggtgctg ggggtgacgg ccttggtggt ggcggcgctg tgggggtttt
     2281 ttaatcacac cctgctgggc aaggccatgc tggccacttc gttcaacagc ctggccgccc
     2341 aattggtggg cgtgcacacc gggcgggtgc tgtttttgag ctttgccatg tctggcgcct
     2401 tgggcgcgct gggcggcgtg ctggtgacgc cgattaccct cacctcttac gacgtgggca
     2461 tcatgctcgg cctgaagggc tttgtcgccg ccgtggtggg cgggctgggc aatggcctgg
     2521 gcgcggtgct gggcgggctg ctggtcgggg tgctggaggc gctgggcgcc ggctatgtgt
     2581 cgtcggctta caaagacgcc atcccgtttg cgctgatctt gctgatgctg ttcttcatgc
     2641 cgcagggcct gttgggcgcg cgcaataccg agcgggtgtg accatgacga cttcccgtta
     2701 tcttcccctg ggcgcgctgg cggtgggctt gctggtgttg ccgtggctgc tgcccaacag
     2761 cttttatctg gacttggcca tccgcatggc gatcaacgcc atcatcgtgc tggggctgaa
     2821 tctgctgatt ggctttgccg ggcaaatcag cctgggccat gctggttttc tgggtctggg
     2881 cgcctatgcc tcggcggtgc tgcccacgca ctttggctgg ccgccgctgg ccgccatgct
     2941 ggccggcatg gcgctgaccg gcgtgctggc ggcgctggtg gcgcggccga tttttcgcct
     3001 gaagggccac tatctggcca tggccacgct ggggctgggc atcatcctca atatcgtggt
     3061 gcgcaatgag gcacagtgga cgggcgggcc agacggcatg ccggtgttgc cattcgaggt
     3121 ggccggcctg acgctgagcc gcgactggca ttggtggtgg ctggtgtcgg ccttgctgct
     3181 gctgagcgtg tggatggcgc aaaacctgat ggattcgccg tttggccgcg ccctgcgcgc
     3241 cttgcacggc tcagaagtcg cctcgcgcgg cgtgggcgtg gatgtggccg gctacaaggt
     3301 ggcgattttt gtgctgtcgg cgatgttcgc cagcctgatg ggcagcatca ccgcgcatta
     3361 cgtcggcttt gtcaccccga atgtggcgga ttttttccac tcgatcgagc tggtcaccat
     3421 ggtggtgatt ggcggcatgg cctcggtgta tggctcgctg ctgggcgccg tgttgctgac
     3481 cgcgctgccg cagctgctgg ccacctttga aggctgggag acggtggtgt ttggcgccat
     3541 cctgatgctg tgcatgatct ttttgcccaa ggggctggcc cccaccctgg cggcgcgctt
     3601 tggcaaggga gggcaatgac atggccttgc ttgaagtcag tgaattgtcc atccagtttg
     3661 gcggcgtcaa ggcggtgcag gcggtcagct tcagcatcga cgccggcatg atttacgcgg
     3721 tgatcggccc caatggcgcc ggcaagacca cgctgttcaa cctgattacc ggcgtgtaca
     3781 cccccaccgc cggcagcatc cgcctggacg gccagccggt gcacggcctg acgcccgacc
     3841 ggctggcgca gcgcggcgtg gcgcgcacct ttcagaacct gcaagtgtgc atgaatatgc
     3901 tggccatcga aaacgtgatg gtgggcgccc acctgcggct ggaccgcagc ctgttcaagg
     3961 cggcgctgcg ctggcccgcc ctgcgccgcg ccgacgcggc cttgcgcgac gaggccgccg
     4021 agctgatgcg ctttgttggc ctggcgcagt acctgaccgc ccgcgccgac agcatgcctt
     4081 atggcgcgct caagcggctg gaaatcgccc gcgcgctggc catgcgcccc cggctgctgt
     4141 ttctggacga acccgccgcc ggccttaacc ccaaggaaac ccaggaagtg gacgcgctgg
     4201 tgcggcgcat tgccgacgcc ggcgtcaccg tggtgctggt cgaacacgat atgaaaatgg
     4261 tgatgaactt gtccgaacgg attctggtgc tggattacgg caagaagctg gccgagggca
     4321 accgcgatga aatccgccgc aacccggatg tgattgccgc ctaccttggc gcccacgcct
     4381 gagctggaga cccctcatgc attctgcttt gcacatcatc caggacgaac accgcaatct
     4441 gtggcgcatc gccatcaccc tggaccgtct gggcgaggac atcgccggcg gcggcgcgct
     4501 ggagccggcg tttttgctgg cgctgtttga ctatctggaa cactttgttg acgccgccca
     4561 ccaccgcaag gaagacgagc atctgtttgc ctgtctgcgc cagcgcgacg ccagcgccat
     4621 cccgctgctg gacgcgctcc aggccgaaca ccgcgaggcc ccggcgcggg tgcaggcgct
     4681 gcgccagcaa ctggccgatt gcctgcacgg ccaaggccag gccagcgcgc tggcgcaggg
     4741 gctgcgcggc tacgccgacg gcctgaagtc gcatatccgc cgcgaagagc agcaggtgtt
     4801 tccgctggcg gaacaggccc tgagcgccgc cgactgggac gccatccagc aggcgttttc
     4861 cagccaccaa gacccgctgt ttggcgacgc cgccagcgcc gaaagccgcg agctgtttca
     4921 ccgcgtggcc agcctggcgc cagaatcggt cggcctgggc ggccacagcg gcggcgagtt
     4981 ggccagccat caggactggc tgctggaagt ggacgcgctg gacagcagct atgggcggat
     5041 tcaggcgctc agcggcattt cgctgaaagt gcgccggggc gaaaccgtgg ccctggtcgg
     5101 cgccaacggc gccggcaaaa ccaccctgct gcgcgcgctg tccggcgtgc agccggcgcg
     5161 cgccgggcaa ttgcgctttg ccggcgaaga catccgccgc ctgcgcgccg acctgcgcgt
     5221 gcgccgtggc atctgccagt cgccagaagg ccgccaggtg tttggcccac tgtccatcga
     5281 agacaatctg cgcctgggcg gctacaccca gcctcgggcg caggccgaca gcgatctgga
     5341 gcaggtgtac gccatgttcc cggtgctcaa ggaaaaacgc cgcctgccgg ccggcacgct
     5401 gtcgggcggc cagcagcaaa tgctggccat cggccgcgcg ctgatgggcc gccccaagct
     5461 tttgctgctg gacgaaccct caatgggcct ggcgccgctg ttggtggaag aagtcttcaa
     5521 tgtggtgaaa accctgaaag cccagggcat gacgctgatt ctggccgagc agaatgcgtt
     5581 ttctgcgctg gcgctggctg atcggggcta tgtgctggaa accggccgca tcaccctgag
     5641 cggcggcggg cgcgaactga tcgacaatga acaggtgcgc gcggcgtatc tgggcatgtg
     5701 agcgccacaa aaaaaccgcg cggtcagccc tgaccgcgcg gcaaactggc catggtctgc
     5761 catgctctct cctcgaccct ggccattacc ccgtgtgttt gcggccgcgt tcagcccggc
     5821 gagcgcttgt agccggtgat catcgcccgg atcaagggct gacgcagctt gcgccccatc
     5881 agcagcgccg ccagcacatg caccgccacc aagccttgca agcctgccga caacgcttcg
     5941 tgcagctctt ccacccactc cacgccaaaa taggcgtcag tgcccatcag ccagccgctg
     6001 accgccaggc caagaatcac gctcagcagg gtgagaatca tcaagcccgc cagcgggtta
     6061 tggcccagat ggttgtccgc ctcgccccgg cgcatggcgc acacatgggc gcgcagacgc
     6121 gccggggtgg gaaaccaatc ggcaaagcgc gcatgccggc tgccgacaaa gccccacagc
     6181 acccgcgcca gcaccgcgcc ggcagcgaaa taacccagcc agcgatgggc gacatcgcct
     6241 tcttccagca caaacagatt cagcgccacg atggcgacca gactccagtg aaacaggcgg
     6301 atgaatccat cccagacttt caccggctga ccggcggcgg gcaattcagc agacatggca
     6361 agctccttgt gacaaaaaat tgcgccgcca gggtcaagat ccggcggcgc aacaagcggg
     6421 cgggcgcggg cagatgcaaa ccgcccggac atgctcactt acttggtttt caccagcttg
     6481 ccgtccaccg gattgaaata ggcctcaacc tttttgccat ccttgtccag gcccttcact
     6541 tcgtagcaat tgtcttccac gcccagcttc ttgatggtgt agcccatgcc cttgacctgg
     6601 gcggtgatct gctccttgct catccagctg ctcttgggcg cgtcggtgca ggattggccg
     6661 gcaaaggcgg acagggaagc gatggacaga acggcggcga gtgcgatgcg ttgcatggaa
     6721 atctcctgaa gtcagtgaca aggccgtgtc aatcacggca tgggagacag tatgcaaggg
     6781 cagacttaag cggaacttag attga
//
LOCUS       DAOPYX010000157         6780 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_13700_length_6780_cov_10.401190, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000157 DAOPYX010000000
VERSION     DAOPYX010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 6780)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6780)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6780
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13700_length_6780_cov_10.401190"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            337..552
                     /locus_tag="PKN55_08885"
     CDS             337..552
                     /locus_tag="PKN55_08885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018747130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HNX71400.1"
                     /translation="MTIQSINVRNQFRGTIKEIIEGPVLSEVDVQTASGIVTSVITTR
                     SVKELQLKVGSEVVAFVKSTEVSIATL"
     gene            575..1165
                     /locus_tag="PKN55_08890"
     CDS             575..1165
                     /locus_tag="PKN55_08890"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011135365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HNX71401.1"
                     /translation="MATPLPDAALEQLFTQARSLHRFTDQPVSDAVIESLYELLKWGP
                     TAFNGQPARYVFVRGADAKARLAPTLSGSNRDKTLAAPVTVIVAYDREFHERLPELFP
                     AFDARGVFAKAPELIEPSARTSATLQAGYLILAARALGLDAGPMTGFDADAVDQAFFA
                     DKRLHSLLLVNLGYGVREGAAPRGPRLAFDEVAHIV"
     gene            1179..1493
                     /locus_tag="PKN55_08895"
     CDS             1179..1493
                     /locus_tag="PKN55_08895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2325 domain-containing protein"
                     /protein_id="HNX71402.1"
                     /translation="MRALIIGGDHVDAAKRALYASGYTEVVHWTGRKNNDLKRPLPTG
                     VTHMVIMLEFVSHALARKMKTTARSQRISVAFVGRKQGGQAEVATRLQSTPDTAALRY
                     AA"
     gene            2203..2706
                     /locus_tag="PKN55_08900"
     CDS             2203..2706
                     /locus_tag="PKN55_08900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018747571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNX71403.1"
                     /translation="MSTATIDDSLDAPLSDLAAHTPADILSQARLRGQQSGAIYAGGV
                     YPPEAWALFQAGAAQLVDVRSAEELKFVGHVPGGQHVAWMTGAALVKNPRFVRELEKI
                     ASKDSVILLLCRSGKRSAAAAEAATLAGFTAVYNVLEGFEGDLDTQQRRGDSGGWRHW
                     GLPWVQD"
     gene            2741..>3693
                     /locus_tag="PKN55_08905"
     CDS             2741..>3693
                     /locus_tag="PKN55_08905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019920422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX71404.1"
                     /translation="MNDSVRAGLSPAPEVFGGQWALADVVARLRVARMSWRKRQGRGR
                     EPGGRELPSREALAEIVHAVCGALFPMRLGPPDLREESEDFYVGHTLDSALNALLAQV
                     KLELNYQARHEARERPDLHQRAVHIVQDFAAALPGLRGLLDDDVMAAFHGDPAARSVD
                     EVLLCYPGILAVLHHRLAHQLYQTGAPLLARMIAELAHAQTGIDIHPGARIGRGFFID
                     HGTGVVIGETAVIGERVRLYQAVTLGAKRFATTEDGSLEKGAPRHPIVEDDVVIYAGA
                     TVLGRITIGRGSSIGGNVWLTRSVPPGSHVTQANVQSEVRH"
     assembly_gap    3694..3793
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3885..4817
                     /locus_tag="PKN55_08910"
     CDS             3885..4817
                     /locus_tag="PKN55_08910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018747568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0140737 - encapsulin nanocompartment
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 2A encapsulin nanocompartment shell
                     protein"
                     /protein_id="HNX71405.1"
                     /translation="MTDYQNIPQQALGDHAARQLANATKTVPQLSTISPRWLVHMLQW
                     LPVEAGIFRLNRVKNASNVQVACSQRDEAELPQTYVDYEQNPREYFLNAVTTVLDVHT
                     RVSDLYSSPHDQIREQLRLTIETIKERQESELINNPEYGLLSNIEPSQRISTLTGAPT
                     PDDLDELLTKVWKEPGFFLAHPLAIAAFGRECTRRGVPPPTVSLFGSQFLTWRGVPLV
                     PSDKLPIVDGKTQILLLRVGEKRQGVVGLFQPGLAGEQSPGLSVRFMGISRNAIASYL
                     ISLYCSLAVLTEDAVAVLDDVEIDKYHDYPNTYK"
     gene            4792..6606
                     /locus_tag="PKN55_08915"
     CDS             4792..6606
                     /locus_tag="PKN55_08915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018747567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0140737 - encapsulin nanocompartment
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 2A encapsulin nanocompartment cargo
                     protein cysteine desulfurase"
                     /protein_id="HNX71406.1"
                     /translation="MTTPTPTSELTASPFDVAGLAQLANAFFASLPGQTPPVAAVQPA
                     APASVTPTALPATPDDAPAPPAELADFGRAGVASYAAPVGFGAGLPDVPAGGPRPHSD
                     SAGAYYFLAENGALGSRSGPSAPADLPPLPAQGDDAVVARAFGLPGEDGLRLLLAEPP
                     RAAPAPAAASAPSYYFLEGDIPRLYAQPAAPSHPGQHSGALDVQAVRRDFPILAERVN
                     GKPLIWLDNAATTHKPQSVIDRISYFYQHENSNIHRAAHELAARATDAYEAARDKVAR
                     FIGAGSSSEIIFVRGATEAINLVANTWGQQNIGAGDEIVVSQLEHHANIVPWQLLAQR
                     VGAVIRVIPVDDSGQILLDEYRKLLNARTKLVAITQVSNALGTITPVETIVALAHAAG
                     ARVLVDGAQSVSHLRVNVRALDADFFVFSGHKVFAPTGIGVVYGKAALLEQMPPWQGG
                     GNMIADVTFERTQYQPAPNKFEAGTGNIADAVGLGAAIDYVERIGLERIAHYEHDLLA
                     YATHGLQRVPGLRLIGTAQHKASVLSFVLPGYRTEEIGAALNREGIAVRSGHHCAQPI
                     LRRFGLEATVRPSLAFYNTCEEVDALVAVVNRLAHGRR"
BASE COUNT         1189 a         2150 c         2143 g         1198 t
ORIGIN      
        1 cctacgcctg cgccggcgat tcatgtttcg caattgcagt gggcgctata agagcggctc
       61 acaacaccca gcgcagcgat cctggctagg cgcacgaccg cagacagtac aacaagtacg
      121 gcaaggtcgg gcaacaacgc caggagggtt ttgttaaccg ctattagagt cgctaacaaa
      181 acccagcgaa gcggccctgg ctaggcgtgt gaccgcagac agtacaagca gtacggcaag
      241 gtcacacaac aacgccaggg ggttttgtta gcggctctaa gccccgctgc gtgtttttga
      301 cagacatccc tctaatactg aataaggact caccccatga ccatccaatc catcaatgtg
      361 cgcaaccagt ttcgcggcac gatcaaggaa atcatcgaag gcccggtgct gtcggaagtc
      421 gatgtgcaaa ccgcctcggg cattgtcacc tcggtgatca ccacccgctc ggtgaaagaa
      481 ctgcaactga aggtcggctc ggaagtggtc gccttcgtca aatcgaccga agtgtcgatc
      541 gccacgctat aacccccgcc caaggagcct caccatggcc acgcccctgc ccgacgccgc
      601 gctggaacaa ctgttcaccc aggcgcgctc actgcaccgt tttaccgatc agccggtcag
      661 cgatgcggtg atcgaatcgc tgtacgagct gctgaaatgg gggccgaccg ccttcaatgg
      721 ccagccggcg cgctatgtgt tcgtgcgcgg cgccgacgcc aaggcgcgcc tggcgccgac
      781 tttgtccggc agcaaccgcg acaagaccct ggccgcgcca gtgacggtga ttgtcgccta
      841 cgaccgcgag ttccacgagc gtctgcccga gctgttcccg gcgtttgacg cgcgcggggt
      901 gttcgccaag gcccccgaat tgatcgagcc cagcgcgcgc accagcgcca ccttgcaggc
      961 gggctatctg attctggcgg cgcgcgcact ggggctggac gccgggccga tgaccggctt
     1021 tgacgccgac gcggtggatc aggcgttttt tgccgacaag cgtttgcatt cgctcttgct
     1081 ggtgaatctg ggctatggcg tgcgcgaagg cgccgcgccg cgcggcccgc gtctggcgtt
     1141 tgacgaggtt gcacacatcg tgtgacgggg aggctgacat gcgggcactg atcatcggcg
     1201 gcgaccacgt tgacgccgcc aaacgcgcgc tgtacgccag cggctacacc gaggtggtgc
     1261 actggaccgg gcgcaagaac aacgacctga agcggccgct gccgaccggc gtcacccata
     1321 tggtcatcat gctggagttt gtcagccatg cgctggcgcg caagatgaaa accacggcgc
     1381 gcagccagcg catcagcgtg gcttttgtcg gccgcaagca aggcggtcag gccgaagtgg
     1441 ccacccgcct gcaatccacg ccggacaccg ccgcgctgcg ttatgcggcg tgagacaaaa
     1501 atagtgtcgg cgcagtcgcg acaggcgttc tgcgccgatg gccacgcctc tgaccgggga
     1561 cactttcttc accgataaca gcctggttaa taccccatca ggtattaccc ctgaatccgc
     1621 tggtgaaggt gcgcccccgg tcagaggcgg gcggtgaatc ctggtttagc ctggcttagt
     1681 gccgctcaca aaactcagcg aagcggtcct ggctaggcgc gcggccgcag acagtacaag
     1741 tcgtacggca aggccgcgta acgacgccag gagggtttgg tgagcagcgc ttagtgcgcc
     1801 aggtttcgtc tcttgcgtac ttttctcatc gcaggccgac cggccagatt gccgtgaatg
     1861 cccacgagcc ccccaaacgg ttccaggttg gccagcatgc cgacaagctg agggtgacac
     1921 gaggtcttgg cgttcatcaa gcgtgggttc ctttggtggg gcggcgcttt cttgccgcga
     1981 gagtctgctg tgtgctggca gctgacaaac gtcatcatag tggccactgt tggcatcgtc
     2041 tgtatctgaa tggtatcggc ctgtaacaat ttatatgctt ttcgcatgtt gattcacggt
     2101 ctttttagat ttattagaga tatagaaatc ctgttttctt acaaaaagaa atatatatgg
     2161 ccggtatagt gaagcacatc cactcttcac aaggatttcg ccatgagcac cgccaccatt
     2221 gatgattctc tcgatgcccc gctgtctgat ctggcggcgc acacccccgc agacattctc
     2281 agccaggcgc gcctgcgcgg ccagcaatcc ggcgccattt acgccggcgg cgtgtatccg
     2341 ccagaagcct gggcgctgtt tcaggccggc gccgcgcagt tggtcgatgt gcgctcagcg
     2401 gaagaactca aattcgtcgg ccatgtgcct ggcggccagc atgtggcgtg gatgaccggc
     2461 gccgcgctgg tgaaaaaccc gcgctttgtc cgcgagctgg aaaaaatcgc cagcaaggac
     2521 agcgtgatcc tgctgctgtg ccgcagcggc aagcgctcgg cggcagcggc agaagccgcc
     2581 accctggccg gttttaccgc ggtgtacaac gtgctggaag gctttgaagg cgacctggac
     2641 acccagcaac gtcgcggcga cagcggcggc tggcgtcact gggggctgcc gtgggtgcag
     2701 gactgagccg acacccatcg catccatacg gagagttgcc atgaatgaca gcgtgcgcgc
     2761 cgggctgagc ccggccccgg aagtgtttgg cggccagtgg gcgctggccg atgtggtggc
     2821 gcggctgcgc gtggcgcgca tgagctggcg caaacgccag gggcgcggac gcgagcccgg
     2881 cgggcgcgaa ctgccctcgc gcgaggcgct ggccgagatt gtccacgccg tgtgcggcgc
     2941 gctgtttccc atgcggctgg gcccgcccga tctgcgcgaa gaaagcgaag atttttacgt
     3001 tggccacacc ctggacagcg cgctgaacgc cctgctggcc caggtcaagc tggagcttaa
     3061 ctatcaggcc cgtcacgagg cgcgcgagcg gccggatctg caccagcggg cggtgcatat
     3121 cgtgcaggac tttgccgctg cgctgcccgg cctgcgcggc ctgctcgacg acgatgtgat
     3181 ggcggcgttt catggcgacc cggccgcgcg cagcgtggac gaagtgctgc tgtgctaccc
     3241 cggcatcctg gccgtgctgc atcaccgcct ggcccatcag ctgtatcaga cgggcgcgcc
     3301 gctgctggcg cggatgattg ccgagctggc gcatgcgcaa accggcatcg acatccaccc
     3361 tggcgcgcgg attggccgtg gattctttat tgatcacggc actggcgtgg tgattggcga
     3421 aaccgcggtg attggcgagc gggtgcggct gtatcaggcg gtgacgctgg gcgccaagcg
     3481 ctttgccacc accgaggacg gctcgctgga aaaaggcgcg ccgcgccatc cgattgttga
     3541 ggatgacgtg gtgatttacg ccggcgccac cgtgctgggg cgcatcacca tcggccgggg
     3601 ctccagcatt ggcggcaatg tctggctgac gcgcagcgtg ccgccgggca gccatgtcac
     3661 tcaggcgaat gtgcaaagcg aggtgcgcca tgannnnnnn nnnnnnnnnn nnnnnnnnnn
     3721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3781 nnnnnnnnnn nnngctgacc gccacccgac actgaatgtt ttccgtggat tttttccccg
     3841 gcaggccccc gtctggccgc cgaatatgac aaggagctta aaacatgaca gactaccaga
     3901 atatcccgca gcaggcgctg ggcgaccatg ccgcccgcca gttggccaac gccaccaaaa
     3961 ccgtgccgca gctgtccacc atctcgccgc gctggctggt gcacatgctg caatggctgc
     4021 cggtggaagc gggtattttc cgcctgaacc gggtgaaaaa cgcctcgaac gtgcaagtgg
     4081 cctgctcgca gcgcgacgaa gccgaactgc cgcaaaccta cgtcgattac gaacaaaacc
     4141 cgcgtgagta tttcctgaac gcggtcacca ccgtgctgga cgtgcacacc cgcgtgtccg
     4201 atctgtacag cagcccgcac gaccagatcc gcgaacagct gcgcctgacc atcgaaacca
     4261 tcaaggagcg tcaggaaagc gaactgatca acaacccgga atacggcctg ctgagcaata
     4321 tcgagccgag ccagcgcatc agcacgctga ccggcgcacc caccccggat gatctggacg
     4381 agctgctgac caaggtgtgg aaagagccgg gcttcttcct ggcccacccg ctggcgattg
     4441 ccgcctttgg ccgcgaatgc acccgccgtg gcgtgccgcc gcccaccgtc agcctgtttg
     4501 gcagccagtt cctgacctgg cgcggcgtgc cgctggtgcc gtcggacaag ctgccgatcg
     4561 tggacggcaa gacgcaaatc ctgttgctgc gcgtgggcga aaagcgccag ggcgtggttg
     4621 gcctgttcca gccgggtctg gctggcgagc aaagcccggg gctgtcggtg cgcttcatgg
     4681 gcatcagccg caacgccatc gcgtcctacc tgatttcgct gtactgctcg ctggctgtgc
     4741 tgaccgaaga cgccgtggcc gtgctcgatg acgtggagat tgacaagtac catgactacc
     4801 ccaacaccta caagtgagct gacggccagc ccgtttgacg tcgccggcct ggcgcaactg
     4861 gccaatgcct tttttgccag cctgcccggc cagacgccgc cggtcgccgc cgtgcagccc
     4921 gccgcccccg ccagcgtgac gcccacagcg ctgcccgcca ccccggacga cgcgccagcg
     4981 ccgccggcgg aactggccga ctttggccgc gctggcgtgg cctcctacgc tgcgccagtg
     5041 ggctttggcg ccggtctgcc cgatgtgccg gcaggcggcc cgcgtccgca cagcgacagc
     5101 gccggggcgt attacttcct ggcggaaaac ggcgcgctgg gcagccgcag cggcccatcg
     5161 gcgccggccg acctgccgcc gctgccggcg cagggcgacg atgccgtggt ggcgcgcgcc
     5221 tttggcctgc cgggcgaaga tggcctgcgc ctgctgctgg ccgaaccgcc gcgcgccgcg
     5281 ccagcgccgg ccgccgccag cgcgccgtcg tattattttc tggagggcga tattccccgg
     5341 ctgtatgccc agccggccgc gcccagtcac cccggccagc acagcggtgc gctggatgtg
     5401 caggcggtgc gccgggattt tcccatcctg gccgagcggg tgaacggcaa gccgctgatc
     5461 tggctggaca atgccgccac cacgcacaag ccgcagtcgg tgattgatcg gatttcgtac
     5521 ttttaccagc acgaaaactc caatatccac cgcgccgcgc atgagctggc ggcgcgcgcc
     5581 accgatgctt acgaggcggc gcgcgacaag gtggcgcgct ttatcggcgc cggctcgtcc
     5641 agcgaaatca tctttgtgcg cggcgccacc gaggccatca acctggtggc caacacctgg
     5701 ggccagcaga acattggcgc gggcgatgaa atcgtggtgt cgcagctgga gcaccacgcc
     5761 aacatcgtgc cgtggcagtt gctggcgcag cgggtggggg cggtgatccg ggtgattccg
     5821 gtggacgaca gcggccagat cctgctggac gaataccgca agctgctgaa tgcgcgcacc
     5881 aagctggtgg ccatcaccca ggtgtccaat gcgctgggca ccatcacccc ggtggaaacc
     5941 attgtcgcgc tggcgcatgc ggcaggcgcg cgcgtgctgg tggatggcgc gcagtcggtg
     6001 tcgcatctgc gcgtcaatgt gcgcgcgctg gacgcggact tctttgtgtt ctccggacac
     6061 aaggtgtttg cgcccaccgg catcggcgtg gtgtacggca aggcggcgct gctggagcag
     6121 atgccgccgt ggcagggcgg cggcaatatg attgccgacg tgactttcga gcgcacgcag
     6181 tatcagcccg cgcccaacaa gtttgaggcc ggcaccggca atatcgccga cgccgtgggc
     6241 ctgggcgccg ccatcgacta cgtcgagcgc atcggcctgg agcgcattgc ccattacgag
     6301 catgatctct tggcctacgc cacccacggc ttgcagcggg ttcccggcct gcggctgatc
     6361 ggcacggcgc aacacaaggc cagcgtgctg tcctttgtgc tgccgggcta ccgcaccgag
     6421 gaaatcggcg cggcgctcaa ccgcgaaggc atcgccgtgc gctctggcca ccattgtgcc
     6481 cagcccatcc tgcgccgttt tggtctggaa gccaccgtgc gcccgtcgct ggcgttttat
     6541 aacacctgcg aggaagtgga cgcgctggtg gcggtggtca accgcctggc gcacggccgc
     6601 cgctgagttg catccaagcc tagggggctt gtgtctgacg ggcatgccag ttggcatgcc
     6661 cttttttcgt ccccattgca tttagaatta ttttaactat aaaagtcata tttttcgttt
     6721 ttttaattct atgcaagcaa ggtacagtgg tcgtgcggct cgtttccgca acctctgatt
//
LOCUS       DAOPYX010000158         6760 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_13756_length_6760_cov_10.283221, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000158 DAOPYX010000000
VERSION     DAOPYX010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 6760)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6760)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6760
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13756_length_6760_cov_10.283221"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..575)
                     /locus_tag="PKN55_08920"
     CDS             complement(<1..575)
                     /locus_tag="PKN55_08920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014088221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HNX71407.1"
                     /translation="MKQSLQLKVSQQLTLTPQLQQSIRLLQLSTLDLQQEIENILHDN
                     PMLERDEGQPDPLPGASETPSNSDVPSEAAPEAQEASRDDDGPADSLELSDWSSSGSG
                     NHDDDFDPHANMVAEKTLREHLLDQLAEHALPPRDRAVVTFLIEELDDDGYLTQSLEE
                     LAAHLPEELGVEPDELQVGLRLLQQFEPAGVG"
     gene            complement(606..1334)
                     /gene="lptB"
                     /locus_tag="PKN55_08925"
     CDS             complement(606..1334)
                     /gene="lptB"
                     /locus_tag="PKN55_08925"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008954913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HNX71408.1"
                     /translation="MTGSVLSVRGLNKRYGKRTVVKDVSLDIASGEVVGLLGPNGAGK
                     TTSFYMIVGLVSADGGEMRLDQHDIRHLPIHQRAKLGLGYLPQEASIFRKMTVEENIL
                     AILEISGRRGEEAEKELTQLLDELNIGHLRESPALALSGGERRRVEIARVLATRPRFI
                     LLDEPFAGVDPIAVLDIQKIIAYLRERQIGVLITDHNVRETLSICDHAYIINEGSVLA
                     KGSPEQLVSDENVRRVYLGEHFRL"
     gene            complement(1331..1894)
                     /gene="lptA"
                     /locus_tag="PKN55_08930"
     CDS             complement(1331..1894)
                     /gene="lptA"
                     /locus_tag="PKN55_08930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008954914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0001530 - lipopolysaccharide binding
                     [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide transport periplasmic protein
                     LptA"
                     /protein_id="HNX71409.1"
                     /translation="MFPPVTDRWLTSLVTLSFVCAAASALAEQGDRDKPIQIEADRAS
                     LDQLKGVTVYEGNVVVTQGTILLRAAQVTVTQDKNGGGDVAVARGNPATFRQKIDGKD
                     EWMDAQGLRIEYDSGKNLVKLFDNARVKRGQDIVIGSTLVYNTKDETFAANGGTGQGA
                     ATNKNAGRVTVILQPKNDGKDNKDKKQ"
     gene            complement(1875..2453)
                     /gene="lptC"
                     /locus_tag="PKN55_08935"
     CDS             complement(1875..2453)
                     /gene="lptC"
                     /locus_tag="PKN55_08935"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HNX71410.1"
                     /translation="MTPRRHVKLFPLVLLALLGGLSFWLQSLTDWLSASVGHDKDAAT
                     PDYVLEGFSATVFERSGGIKQHLTGERAWQLPNDPTIHLLAPHLRQYQRGVLHLEIIG
                     TDGLYNPKTRISVFPREVTLLRYAPGLPTAKLVTSRLTVDTEKRTAQTPAPAVLERGA
                     SVVRTTGFTYNDATQQVQLLSKVKVTHVPTRH"
     gene            complement(2450..2974)
                     /gene="kdsC"
                     /locus_tag="PKN55_08940"
     CDS             complement(2450..2974)
                     /gene="kdsC"
                     /locus_tag="PKN55_08940"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014088220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HNX71411.1"
                     /translation="MQNVHTLARAVKMLVLDVDGVLTDGSIYLTDSGEEFKAFNAQDG
                     LGMTLLRQSGVTLAIISGRNARCVEVRAQALGVAHLYQGIGNKAEAFAQLLADTGLTP
                     AECAYIGDDVIDLPVMRQVGFAVAVPAAPHLVQSHAHYVTGSSGGRGAVREVCELILQ
                     AQGKLDAALARYLA"
     gene            complement(2988..3971)
                     /locus_tag="PKN55_08945"
     CDS             complement(2988..3971)
                     /locus_tag="PKN55_08945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003822881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HNX71412.1"
                     /translation="MTDDAVSLRRLALARDVLATEADAILNVSARLDAVFLRAVDAAL
                     ACRGRLVVTGMGKSGHIGRKIAATLASTGTPAFFMHPAEAAHGDLGMITGDDMVLALS
                     NSGETAEVVALLPALRRKGVSLLAITGRPESTLAREADWHLDAAVEREACPLGLAPTS
                     STTAALALGDALALTLLDARDFREEDFALSHPGGSLGKRLLVRVADVMHSGPALPVVR
                     PDTRLKDALYEISRKGLGMTAVCDDDGQLVGVFTDGDLRRLLDRDAFDLDGPVGAVMN
                     RAPRHIGAERLAAEALQRMQQDKINGLLALDAAGRLTGALNMHDLLRAGVV"
     assembly_gap    4369..4468
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4595..6568
                     /locus_tag="PKN55_08950"
     CDS             4595..6568
                     /locus_tag="PKN55_08950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008954918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HNX71413.1"
                     /translation="MHSLAPVVAILLAAVLVVVLCRLLRVPAMLGYLLVGFIVGPGGL
                     QLIADTAQTRFLGEIGIVFLMFTIGLEFSLPKLRAMRHIVFGLGAAQVVVTLLVVMAL
                     VVLAGASPLTGFALGSVAALSSTAIVSKLLTERLELNAPHGQLSIGVLLFQDLAVVPL
                     LILIPAFASHSTHLWQDLGLAALKVMGVMVLLFSIGQRVMRPWFHLVARQRSSELFMI
                     NVLLVTLGVSYLTELSGLSLALGAFVAGMLIAETEYRYQVEEDIKPFRDILLGFFFVT
                     IGMRLDVSVLTEQFTLVLVFALFLVLGKALLMFAIARLFQYKSGESLRSAVALAQGGE
                     FGFVLLALAGNLDLIAPQVEQSAIAAVLFSMLASPFLIPRSDWLVRKLIRADWMMQAA
                     DLHHILVSSMLRSEHVLICGYGRSGQALARLLERESIPVFALDLDPERVREAAAAGDA
                     VVFGDAAKKEVLLAAGLMRAKALVITYSDTHSSIRILQVVQQLRPDLTVIVRTVDDSD
                     IDLLREAGADEVVAEVMEGSLMLASQALMALGVPLNRVLRRIRSVREERYSLFRGFFR
                     GVTDESLAMDDAQQARLAAVTLTPGSAAVGKTLGELHLGALCVQVRTVKRRSARHIAP
                     DLDFELHADDVLVLLGTPEQIATAELLLLGGKH"
BASE COUNT         1066 a         2129 c         2094 g         1371 t
ORIGIN      
        1 cccaccccgg ccggctcgaa ctgctgcaac aggcgcaggc ccacttgcag ctcgtccggc
       61 tcgacgccca gttcttccgg caggtgggcg gccagctctt ccagcgattg ggtgagatac
      121 ccgtcgtcgt ccagctcttc aatcaggaag gtcaccaccg cgcggtcgcg cggcggcagc
      181 gcgtgttcgg ccagttgatc cagcaaatgc tcgcgcaggg ttttttccgc caccatattg
      241 gcgtgcgggt cgaaatcatc gtcgtggttg cccgagccgc tgctggacca atccgacaac
      301 tccaggctgt cggccggacc atcgtcgtcg cggctggctt cctgcgcctc gggcgcggcc
      361 tcgctgggca catcgctgtt gctgggtgtt tcacttgcgc cgggcagcgg gtcgggctgg
      421 ccttcgtcac gctccagcat cgggttgtcg tgcaggatgt tttcgatttc ctgctgcaaa
      481 tccagtgtgg agagctgcaa tagccggatc gactgttgca gttgcggggt aagcgtcagc
      541 tgctggctga ccttgagttg caggctttgt ttcatgggcg ccgctctgtc atgctcgtca
      601 ctcgcctaca ggcggaaatg ttcaccaaga tacacgcgtc tgacgttttc gtcgctgacc
      661 agttgctccg ggctgccttt ggccagcacc gagccttcat tgataatgta ggcatggtcg
      721 caaatcgaca aggtttcgcg cacattatgg tcggtgatca gcacgccaat ctggcgctcg
      781 cgcaggtagg cgatgatttt ctggatatcc agcacggcga tcggatccac cccggcaaag
      841 ggttcatcca gcaggataaa acgcggacgg gtggccagca cgcgggcgat ttccacccgc
      901 cgacgctcgc cgcccgataa ggccagcgct ggcgattcgc gcagatggcc gatgttcagc
      961 tcatccagca actgggtgag ttctttttct gcttcttcac cccgacggcc tgaaatttcc
     1021 agaatggcca ggatgttttc ttccacggtc attttgcgaa agatcgacgc ttcctgcggc
     1081 aaatagccca gcccgagttt ggcgcgctgg tggatgggca gatggcggat gtcgtgctgg
     1141 tccaggcgca tttcgccgcc atcggcgctg accaggccaa caatcatgta aaaactggtg
     1201 gtcttgccgg cgccattggg ccccaacagc cccaccactt cgccgctggc gatgtccagc
     1261 gacacatcct tgaccaccgt gcgtttgccg tagcgcttgt tcaggccacg caccgaaagc
     1321 acactgccgg tcattgtttt ttgtccttgt tgtccttgcc gtcgttcttc ggctgcagga
     1381 tcactgtcac ccgcccggca tttttattgg tggctgcgcc ctggccggtg ccaccattgg
     1441 cggcaaaggt ttcgtccttg gtgttataga ccagggtcga accgatcacg atgtcctggc
     1501 cacgcttgac gcgggcattg tcgaacagct tcaccagatt cttgccgctg tcgtattcga
     1561 tgcgcaggcc ttgggcatcc atccattcgt ccttgccgtc gatcttctgg cggaaggtgg
     1621 ccgggttgcc gcgcgccacc gccacatcac cgccgccatt tttgtcctgg gtgacagtga
     1681 cttgagcggc gcgcagcaag atggtgccct gggtgaccac cacattgcct tcgtagacgg
     1741 tgacgccctt gagctgatcc aggctggcgc ggtcagcctc gatctggatg ggtttgtcgc
     1801 ggtcgccttg ctcggccagc gcgctagctg ctgcgcagac aaaactcagg gtgacaaggc
     1861 tggtcagcca tcggtcagtg acgggtggga acatgggtta cctttacctt ggacagcagt
     1921 tgcacctgtt gggtggcatc gttatatgta aagccggtag tgcgcaccac gctggcgccg
     1981 cgctccagca cggcgggcgc cggggtttgc gccgtgcgtt tttcggtatc gacggtcagc
     2041 cggctggtga ccagcttggc ggtgggcagg cccggtgcgt agcgcagcag agtgacttca
     2101 cgcggaaaca cgctgatgcg ggttttcggg ttgtacaagc catcggtgcc gatgatttcc
     2161 agatgcagca cgccacgctg atattggcgc aggtgtggcg ccagcaggtg gatggtgggg
     2221 tcattgggca attgccaggc gcgctcgcct gtcagatgct gcttgatgcc gccactgcgt
     2281 tcaaacaccg tggcagaaaa cccttccagc acataatccg gcgtggcggc gtccttgtcg
     2341 tgcccgacgc tggcgctgag ccagtcggtc agcgattgca gccagaagct caggccgccc
     2401 agcagcgcca gcaacaccag cgggaacagc ttgacgtgac gacgcggcgt catgccagat
     2461 accgcgccag cgccgcgtcc agcttgcctt gcgcttgcag aatcagttcg cagacttctc
     2521 gcaccgcgcc gcgcccgccg ctgctgcccg tcacataatg ggcgtggctt tgtaccagat
     2581 gcggcgccgc cggcaccgcc acggcaaagc ccacctggcg catcaccggc aaatcaatca
     2641 catcgtcgcc gatataggcg cactccgccg gggtcagccc ggtgtcggcc agcagttggg
     2701 caaaagcctc ggctttattg ccgatgccct gatacaaatg cgccacgccc agcgcctggg
     2761 cgcgcacttc cacacagcgg gcgttgcggc cgctgatgat ggccagcgtc acgccgcttt
     2821 gccgcagcag ggtcatgccc aggccgtcct gagcgttaaa cgccttgaat tcttcgccgc
     2881 tgtcggtcag atagatgctg ccgtcggtga gcacgccatc gacgtccagc accagcatct
     2941 tcaccgcgcg ggcgagggta tgcacgtttt gcatgaaaaa tcctggctca caccacacca
     3001 gcgcgcagca ggtcgtgcat attcagcgcg cctgtcagcc ggccggcggc gtcgagcgcc
     3061 aaaagtccgt tgatcttgtc ttgttgcatg cgttgcagcg cttccgccgc cagccgttcg
     3121 gcgccgatat ggcgcggcgc gcggttcatc accgcgccca ccgggccatc caggtcaaac
     3181 gcgtcgcgat ccagcagccg gcgcaaatcg ccatcggtga acaccccgac caactggcca
     3241 tcgtcgtcgc ataccgcggt catgcccagg cccttgcggc tgatttcgta cagtgcgtct
     3301 ttcagccggg tgtccgggcg caccaccggc agcgccgggc cgctgtgcat cacatccgcc
     3361 acccgcacca acaggcgctt gcccagactg ccgcccggat gcgacagggc aaagtcttcc
     3421 tcgcgaaaat cgcgcgcatc cagcagggtc agcgccagcg cgtcgcccag ggccagcgcc
     3481 gccgtggtgc tggaggtcgg cgccagcccc agcgggcagg cttcgcgctc gaccgccgcg
     3541 tccagatgcc aatccgcctc gcgcgccagg gtggattccg gccggccggt gatggccagc
     3601 aagctcaccc ccttgcggcg cagcgccggc aacaaggcca ccacctcggc ggtttcgccg
     3661 gagttggaca aggccagcac catatcgtca ccggtgatca tgcccaaatc gccatgcgcg
     3721 gcctcggccg ggtgcatgaa aaatgccggc gtgccggtgg aggccagcgt ggcggcgatt
     3781 ttgcggccaa tatgcccgga cttgcccatg ccggtcacca ccaagcgccc acgacacgcc
     3841 agcgcggcgt ccaccgcgcg caaaaacacc gcgtccaggc gggcggaaac attcaaaatg
     3901 gcgtcggctt cggtggccag cacatcgcgg gccagcgcca gccggcgcaa ggaaacagcg
     3961 tcgtcggtca tacggcagtt acacggcaag tgaccgtggt caaagaacgc aagtataacc
     4021 aagttgcctg cctgccgcat ggcgcgggcg atggacgggg tggccttggc gctgtgtggg
     4081 tgtcgtgagc ctggatcaac gccggctggc gccaaagcgg caagccctcg tcaactatct
     4141 gctgtccctg cggggcgacg ccaaaacgcc ggttcgcgac ggcgcgcgcg cttgcgcaaa
     4201 atcacctcct gagcatcggc gcggcgccat tccgatttat gtcaaaagta tattttttaa
     4261 caaacttgtc tcatgccgcc cccatcggtt atacatgtgc gcctagagtc gctcacaaaa
     4321 ctttcctgcc gtcgttgcgc agccttgccg cactgcttag agccgctcnn nnnnnnnnnn
     4381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnga gccgctcttg gtgctgtctg cggccgcgcg
     4501 cctagccagg atcgctacgc tgagttttgt gagcgactct tatctacaga agaatgtgcc
     4561 ggccgtttgc gccccacgga gaaaatcgtc gttcatgcat tcgctcgccc ccgttgtcgc
     4621 catcttgctc gccgccgtgt tggtggtggt cctctgccgc ctgttgcgcg tgccggccat
     4681 gctgggctat ctgctggtgg gcttcatcgt cggccccggc gggctgcaac tgatcgccga
     4741 caccgcgcaa acccgttttc tgggtgaaat cggcattgtg tttctgatgt tcaccattgg
     4801 cctggaattc agcctgccca agctgcgcgc catgcgccat atcgtgtttg gcctgggcgc
     4861 ggcgcaagtg gtggtcacct tgttggtggt gatggcgctg gtggtgctgg ccggcgcttc
     4921 gccgctgacc ggctttgccc tgggctcggt ggcggcgctg tcgtccacgg cgattgtctc
     4981 caagctgctc accgagcggc tggagctcaa cgccccgcat ggtcagctct cgattggcgt
     5041 gctgctgttt caggatctgg ccgtggtgcc cttgctgatt ctgatccccg cctttgcctc
     5101 gcattccacc cacctgtggc aagacctcgg cctggcggcg ctgaaggtga tgggcgtgat
     5161 ggtgctgttg ttcagcatcg gccagcgggt gatgcggccg tggtttcatc tggtggcgcg
     5221 ccagcgttcg tccgagctgt tcatgatcaa tgtgctgctg gtcacgctgg gggtgtctta
     5281 cctgactgag ctctccggcc tgtcgctggc gctgggggcg tttgtcgccg gcatgctgat
     5341 tgccgaaacc gaataccgct atcaggtgga agaagacatc aagcccttcc gcgatatcct
     5401 gctgggcttt ttctttgtca ccatcggcat gcgcctggat gtgtcggtgc tgaccgagca
     5461 attcaccctg gtgctggtgt ttgcgctgtt tttggtgctg ggcaaggcgc tgttgatgtt
     5521 cgccatcgcc cggctgtttc agtacaaatc cggtgagtca ctgcgcagcg cggtggcgct
     5581 ggcgcagggc ggcgagtttg gttttgtgct gctggcgctg gccggcaacc tggatttgat
     5641 cgccccgcaa gttgagcaat cggccatcgc cgccgtgttg ttctccatgc tggcgtcacc
     5701 gtttttgatt ccgcgcagcg actggctggt gcgcaagctg attcgcgccg actggatgat
     5761 gcaggcggcg gatttgcacc atattctggt gtccagcatg ctgcgcagcg agcatgtgtt
     5821 gatttgcggc tatggccgca gcggccaggc gctggcgcgg ctgctggagc gcgaatccat
     5881 cccggtgttt gccctcgatc tggacccgga gcgggtgcgc gaggccgccg ccgctggcga
     5941 cgccgtggtg tttggcgacg ccgccaaaaa agaagtgctg ctggccgccg gcctgatgcg
     6001 cgccaaggcg ctggtgatca cctattccga cacccattcg tcgatccgca ttctgcaagt
     6061 ggtgcagcaa ctgcgccccg acctcaccgt gattgtgcgc accgtggatg actccgacat
     6121 cgacctgctg cgcgaagccg gcgccgacga agtggtggcc gaagtgatgg aaggcagcct
     6181 gatgctggcc tcgcaagcgc tgatggcgct gggcgtgccg cttaaccgcg tgctgcgccg
     6241 catccgcagc gtgcgcgaag aacgctacag cctgtttcgc ggctttttcc gtggcgtgac
     6301 tgacgaaagc ctggccatgg acgacgccca acaagcccgc ctggccgccg tcaccctcac
     6361 ccccggctcc gccgccgtgg gcaaaaccct gggcgagctg cacctgggcg cgctgtgcgt
     6421 gcaagtgcgc acggtaaaac gccgctccgc ccgccacatc gcccccgacc tggattttga
     6481 actgcacgcc gatgatgtgc tggtgctgct gggcacgccg gagcagattg ccacggcaga
     6541 gttgctgttg ttgggcggga agcactaaga gcggctcaca aaaccctcct ggcgttgttg
     6601 cccgaccttg ccgtacttct tgtactgtct gcggccgtgc gcctagccag gatcgctgcg
     6661 ctgggttgtg ttagccgctc tgagcgcttg aatgctggcg gcgtcagcgg ttggatggca
     6721 gagcgccgcc aatcgcacgc aaataatcgg cgatttcgcc
//
LOCUS       DAOPYX010000159         6754 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_13778_length_6754_cov_10.192715, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000159 DAOPYX010000000
VERSION     DAOPYX010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 6754)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6754)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6754
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13778_length_6754_cov_10.192715"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2114)
                     /locus_tag="PKN55_08955"
     CDS             complement(<1..2114)
                     /locus_tag="PKN55_08955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX71414.1"
                     /translation="MLFACRRFAMSALSDLPTIDPAAVTAFITRWKTAAGGSERANYQ
                     LFIVELCQLLGVPSPDPSGEDSRDHAYVCERRVVFRHGNGSVSQGFIDCYKRGCFVLE
                     AKGSRKADGQVLSRKMEEARSQAQNYARALPADSEGRPPFLLIVDVGKTFELYAEFTR
                     SGAEHTPFPDAQNHRIPLAGLARPEVQARLRALWLDPMSLDPSRQSARATREIADMLA
                     QLAKRLETRHPAEEVAAFLTRCLFTFFAEDMRLLPERSFTELLESLQHTPELFVDMVE
                     NVWSSMDTGGFCAALRSKLLHFNGKLFKTPHALALDRDEITALVRAGRADWTQVEPAI
                     FGALLERALNPDERHSLGAHYTPRAYVERLVLPTVIEPLRRDWENVRAAALLLGNEDK
                     PADAVALLHEFHYRLCHITVLDPACGSGNFLYVALEHIKRLEGEVLDMLHHTFQQTSR
                     LEGEGLTVDPHQFLGLELNPRAAAMAELVLWIGYLQWHFRTHGHAQPPQPVLRDFKNI
                     ECRDAVLSWSQIDPLLDANGQPCSRWDGKTWKTHPVTGERVPDDSARLPLYRYQHPRP
                     ADWPPADFIIGNPPFIGAGPMRAALGDGYVEALRSAYPDVPDSADLVMFWWEKAANAV
                     RAGHSRRFGLITTNSLKQTFNRRVVQRHLQADPPLKLSFAIPDHPWVDSSDGAAVRIA
                     MTVGSSDKQAGVLQTVTKEWNAG"
     gene            complement(2215..3021)
                     /gene="dapB"
                     /locus_tag="PKN55_08960"
     CDS             complement(2215..3021)
                     /gene="dapB"
                     /locus_tag="PKN55_08960"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011135347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HNX71415.1"
                     /translation="MSELNLVIVGAGGRMGRALIESTLNTPGCRLHAALERADSPFLG
                     QDAGLFSGQTTGVTITADLAAALQGADLLIDFTRPEGTLAYLAACRAAGVKMVIGTTG
                     FDDAGKAAIAEAAKEISIVFAANYSVGVNLTFKLLEVASRVLADGFDVEIIEAHHRYK
                     VDAPSGTALRMGEVIADTLGRDLKTCAVYGREGITGERDPNTIGFATVRGGDVVGDHT
                     VLFAALGERVEISHKASSRATFANGAVRAARWLGDKTSGLFDMQDVLQLK"
     gene            complement(3025..3411)
                     /locus_tag="PKN55_08965"
     CDS             complement(3025..3411)
                     /locus_tag="PKN55_08965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008954359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HNX71416.1"
                     /translation="MRKILLVSGVLLLTACGSYSPLNWLTPHTIDVQQGNYVTQDVVD
                     KLQPGMTQAQVRFLLGTPLMADMFHANRWDYVYTLRNGSTLAESRRLSVFFDAEGKLS
                     KVEGSAMPARRATLDSTPAAAPEGKQ"
     gene            3516..3959
                     /gene="fur"
                     /locus_tag="PKN55_08970"
     CDS             3516..3959
                     /gene="fur"
                     /locus_tag="PKN55_08970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011135349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HNX71417.1"
                     /translation="MNKASHLKDIGLKATGPRLKILDLFERSSARHLSAEDVYRLLLA
                     EEVDIGLATVYRVLTQFEQAGILVRHHFESGKAVYELDEGAHHDHMVCMSCGRVTEFF
                     SPEIEELQNKIAEQHNFRIREHSLYLYGECKDCACTGKSAGKLRG"
     gene            4020..4727
                     /gene="aat"
                     /locus_tag="PKN55_08975"
     CDS             4020..4727
                     /gene="aat"
                     /locus_tag="PKN55_08975"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014087913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HNX71418.1"
                     /translation="MIPWLSPHPSFPPVAQALSEPDGLLAAGGDLSTARLLAAYRRGI
                     FPWYSPGDPILWWAPSQRMVLHTAQLRVPRSFAKVLRNRRYQVVFDRDFEAVIRACAA
                     PRDGQNGTWIVEDMIQAYLRLFRAGHAHSVETWQDGQLVGGLYAVNVGRMVYGESMFS
                     RAPDASKIALAHLARHLHGHGLDLIDCQMHTGHLASMGARLMPRDDFIAAVAARVAAP
                     VPAGVWEYCFDNEPPRP"
     gene            4708..5457
                     /locus_tag="PKN55_08980"
     CDS             4708..5457
                     /locus_tag="PKN55_08980"
                     /EC_number="2.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008954362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004057 - arginyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0016598 - protein arginylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginyltransferase"
                     /protein_id="HNX71419.1"
                     /translation="MSHRDHNRIFSIHFYATAPYPCSYLPGRQARSQVAIPADAIDDA
                     VYSQLVHIGFRRSGLFTYRPYCDDCDACVSVRIPVARFVANRTQRRVWRRHAGRLFAV
                     RRELRFEMEHYALYRRYQASRHADGSMADDNHHQYAEFILKSGVNSELVEFRDEHGQL
                     KMVSLIDQLADGLSAVYTFFEPDDASASYGVYNVLWQIEETRLRGLSYLYLGYWIAEC
                     RKMAYKTIYRPLERLSHGRWMEMATPDQASD"
     gene            5570..6556
                     /gene="dusA"
                     /locus_tag="PKN55_08985"
     CDS             5570..6556
                     /gene="dusA"
                     /locus_tag="PKN55_08985"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003744662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HNX71420.1"
                     /translation="MPAVTLPPRRLAVAPMLDWTDRHYRYFARHITRHTWLYTEMVTT
                     GALIHGDIPRHLRFDPMESPIALQLGGSEPADLAHCAKLAENWGYDEVNLNVGCPSER
                     VQRGAFGACLMNEAALVADCVKAMRDAVSIPVTVKHRIGVDQIEHYGFLAEFVDTVAA
                     AGCTTFIVHARNAILKGLSPKENREIPPLKYDYVYRLKQERPELEILINGGVRTLEDI
                     DTHLQHVDGVMIGREAYHNPYLMAAFDARYYGDDHPIPSREDILAAITPYIARELADG
                     NRMNNLTRHILGLYQGQPGARRFRQLLSDSRRLAEKRVALLQEACPTAGGKA"
BASE COUNT         1235 a         2098 c         2181 g         1240 t
ORIGIN      
        1 cccgcattcc attctttggt gacggtttgc agcacgccgg cttgtttgtc gctgctgccg
       61 acggtcatgg caatgcgcac ggcagcgcca tcgctggaat ccacccaggg gtgatcgggg
      121 atggcaaagc tgagttttag cggcgggtcg gcttgcaaat ggcgctgcac cacacggcgg
      181 ttaaaggttt gtttcaggct gttggtggtg atcaggccaa agcggcggct atggccggcg
      241 cgcacggcgt tggcggcttt ttcccaccag aacatcacca gatcggcaga gtccggcaca
      301 tccgggtagg cgctgcgcag ggcctccaca tagccatcgc ccagcgcggc gcgcattggg
      361 cctgcgccaa taaatggcgg attgccaatg atgaaatccg ccggcggcca atctgccggg
      421 cgggggtgct gatagcgata caagggcagg cgggcgctgt cgtcgggcac gcgctcgccg
      481 gtcaccgggt gggttttcca ggttttgcca tcccagcggc tgcatggctg accgttggcg
      541 tccagcaggg ggtcgatctg gctccagctt aatacggcat cgcggcattc gatgttttta
      601 aagtcacgca acaccggctg cggcggctgg gcgtggccgt gggtgcggaa gtgccattgc
      661 agatagccaa tccacagcac cagctcggcc atggcggcgg cgcgcgggtt aagctccagc
      721 cccaaaaact ggtgtgggtc tacggtcagg ccctcgcctt ccagccggct ggtttgctga
      781 aaagtgtggt gcagcatgtc cagcacttcg ccttccaggc gtttgatgtg ctccagcgcc
      841 acatacaaaa aattgcccga gccgcaagcg ggatcaagca cggtgatatg gcacaggcga
      901 tagtgaaatt cgtgcagcag ggccacggcg tcggcgggtt tgtcttcgtt gcccagcagc
      961 agggcagcag cgcggacgtt ttcccagtcg cggcgcagcg gctcaatcac ggtgggcagc
     1021 accagccgtt caacgtaggc gcgcggggtg tagtgggcgc ccagactgtg gcgctcgtcc
     1081 gggttgagcg cgcgctccag cagcgcgcca aagatggcgg gttcgacttg cgtccagtcg
     1141 gcgcggccgg cgcgcaccag ggcggtgatt tcatcgcggt ccagcgccag cgcatgcggg
     1201 gttttgaaca gtttgccgtt aaagtgcagc agtttgctgc gcagggcggc gcaaaaaccg
     1261 ccggtgtcca tgctgctcca gacgttttcc accatatcaa caaacaattc tggggtgtgt
     1321 tgcaggcttt ccagcagttc ggtaaagctg cgctcgggca gcaggcgcat gtcttcagca
     1381 aaaaaggtaa acaggcagcg ggtgagaaac gcggccactt cttcggcggg gtggcgggtt
     1441 tccaggcgct tggccagttg ggccagcatg tcggcaattt cacgggtggc gcgggcggat
     1501 tggcgggagg ggtccagcga catggggtca agccacagcg cacgcagccg cgcctggact
     1561 tctgggcgag ccagcccggc cagcgggatg cggtggtttt gcgcatccgg gaacggggta
     1621 tgttcggcgc cgctgcgggt aaattcggcg taaagctcaa acgttttacc cacatcgaca
     1681 atcagcaaaa acggcggacg gccttcactg tcggcaggca gggcgcgggc gtagttttgg
     1741 gcctggctgc gggcttcttc cattttgcgt gacagcacct gaccatcggc cttgcgcgag
     1801 cctttggctt ccagcacaaa gcagccgcgt ttgtagcagt cgataaaccc ttggctgacg
     1861 ctgccattgc catggcgaaa caccacgcgg cgctcgcaca cataggcgtg gtcacggcta
     1921 tcttcgccag aagggtcggg cgagggcacg cccagcagtt ggcagagctc aacaataaac
     1981 agctggtagt tggcgcgctc ggagccgccg gcagcggttt tccagcgggt gataaaggcc
     2041 gtgacggcgg cggggtctat ggtgggcagg tcggacaatg cggacatggc aaagcggcgg
     2101 caggcaaaaa gcataatata cctgattcat atgccccagg ctgcggccaa aaacaacacg
     2161 gcgcttcgcg caaagctgcg ggaagcgccg tgtgggcagg tcggcaagct gggtttactt
     2221 cagttgcaac acgtcctgca tgtcgaacag accgctggtt ttgtcgccca gccagcgggc
     2281 ggcgcgcacg gcgccgttgg cgaaggtggc gcggctggag gctttgtggc tgatttccac
     2341 acgctcgccc agggcggcaa acagcacggt gtggtcgccc accacgtcgc cgccgcgcac
     2401 ggtggcaaag ccgatggtgt tggggtcgcg ctcgccagtg atgccttcgc ggccgtacac
     2461 ggcgcaggtt ttcaggtcgc ggcccagggt gtcggcgatg acttcgccca tgcgcagcgc
     2521 ggtgccggag ggggcgtcca ctttgtagcg gtggtgagct tcgatgattt cgacgtcaaa
     2581 gccgtcggcc agcacgcgcg aggcgacttc cagcagtttg aaggtgaggt tgacgcccac
     2641 tgagtagttg gcggcaaaca caatgctgat ttctttggcg gcttcggcga tggcggcctt
     2701 gccggcgtcg tcaaagccgg tggtgccaat caccattttc acgccagcgg cgcggcaggc
     2761 ggccagatag gccagcgtgc cttccgggcg ggtgaagtcg atcagcagat cggcgccttg
     2821 cagcgcggcg gccaggtcgg cggtgatggt cacgccagtg gtctggccgc tgaacaggcc
     2881 ggcgtcctgg cccagaaacg ggctgtcggc gcgttccagc gcggcgtgca gccggcagcc
     2941 gggggtgttg agggtggatt caatcagcgc gcggcccatg cggccaccag cgccgacgat
     3001 gacgagattc agctcagaca tggtttactg ctttccttca ggggcggcgg cgggggtgct
     3061 gtccagggtg gcgcgacgcg ccggcatggc gctgccttcc accttgctga gcttgccttc
     3121 cgcgtcaaaa aacaccgaca gacgccgcga ttcggccagg gtggagccat tgcgcagggt
     3181 atagacatag tcccaacggt tggcgtggaa catgtctgcc atcagcgggg tgcccagcag
     3241 aaagcgcacc tgcgcctggg tcatgcccgg ctggagcttg tccaccacat cctgggtgac
     3301 gtagttgccc tgctgcacat caatggtgtg aggggtgagc caattcaatg gcgaataact
     3361 gccacatgcg gtcagcagca gcacgccgga caccagcaaa atttttcgca tgacggatcg
     3421 gaggatggtt gacgggagag ggccgggcgg cttgattagg caacagcccg aaaacgctat
     3481 atgataactt acccagccca aaatacgata cgtccatgaa caaggcaagt catctcaagg
     3541 atattggcct gaaggccact ggcccccgcc tgaaaatcct cgatctgttc gagcgcagca
     3601 gcgcccgtca tctcagcgca gaagacgtct accgtctgct gctggccgaa gaagtcgata
     3661 tcggcctggc cacggtctac cgcgtgctca cccagtttga gcaggctggc attctggtgc
     3721 gccatcactt tgaatccggc aaggcggtgt acgagctgga cgaaggcgcg caccacgacc
     3781 atatggtgtg catgagctgc ggccgtgtga ccgagttttt cagcccggaa atcgaagagc
     3841 tgcaaaacaa gatcgccgag cagcacaact tccgcatccg cgagcattcg ctgtatctgt
     3901 acggcgaatg caaggattgc gcctgcacgg gtaaatccgc aggcaaattg cgcggctgac
     3961 gctgatccct ttcggcgcgc cgcccggcgc gtcggcgctt ttgtgtggcg acactgccga
     4021 tgattccctg gttgagcccc cacccgagct ttccgccggt cgctcaggcg ctgagcgagc
     4081 ctgatggcct gttggctgcg ggcggcgatt tatccaccgc ccgcctgctg gccgcttatc
     4141 ggcgcggtat ttttccctgg tatagccctg gcgacccgat tttgtggtgg gcgccgtcgc
     4201 agcgcatggt gctgcacacg gcccagctgc gcgtgccgcg ctcgtttgcc aaggtgttgc
     4261 gcaaccggcg ctatcaagtg gtgttcgacc gcgatttcga ggcggtgatc cgcgcctgcg
     4321 ccgccccacg cgatggccag aacggcacct ggattgtcga agacatgatc caggcttatc
     4381 tgcggctgtt tcgcgccggc cacgctcatt cggtggaaac ctggcaggat ggccaactgg
     4441 taggcggctt gtatgccgtg aacgtggggc ggatggtcta tggtgaatcc atgttctcgc
     4501 gcgcgccaga cgcctccaaa attgcgctgg cgcacctggc ccgacatttg cacgggcatg
     4561 ggctggactt gatcgactgc cagatgcaca ccgggcatct ggccagcatg ggggcgcggc
     4621 tgatgccccg cgatgacttt atcgcggcag tggctgcgcg cgtggccgcg cctgtgccgg
     4681 ccggcgtctg ggagtattgt ttcgacaatg agccaccgcg accataaccg gattttcagc
     4741 atccactttt acgccaccgc gccgtatccg tgcagctatc tgcccggccg tcaggcgcgc
     4801 tcgcaagtgg cgattccggc cgacgccatc gacgatgcgg tatatagcca gctggtgcat
     4861 atcggctttc gccgcagcgg gctgtttacc tatcggccgt attgcgacga ctgcgacgcc
     4921 tgcgtatcgg tgcgcatccc ggtggcgcgc tttgtcgcca accgcaccca gcgccgggta
     4981 tggcgtcgcc acgccggccg gttgtttgcc gttcgccgcg agctgcgctt tgaaatggag
     5041 cactacgcgc tgtatcggcg ctaccaggcc tcgcgccacg ccgacggcag catggccgac
     5101 gacaaccacc accaatacgc cgaattcatc ctgaaaagcg gagtaaacag cgagctggtg
     5161 gaattccgcg acgaacatgg ccagctcaag atggtcagcc tgattgacca actggccgat
     5221 ggcctgtcgg cggtgtacac cttttttgag cctgacgacg catccgccag ctacggcgtg
     5281 tacaacgtgc tctggcaaat cgaagaaacc cgcctgcgcg ggctgtcgta tctgtatctg
     5341 ggctactgga tcgccgaatg ccgcaagatg gcctacaaaa ccatttaccg accgctggag
     5401 cgcctgagtc acgggcgctg gatggaaatg gccacgcctg accaagccag cgactgaaac
     5461 agcccccgca cacgcttgac cctggcgtgc gcgccaccgg ataattcgcg gtttcattac
     5521 aaagacttgc gcagcccttg ccgactgcgc tgtcgctcac gctgatttca tgcctgcagt
     5581 caccctgccc ccccgccgtc tggccgtcgc ccccatgctg gactggaccg accgccatta
     5641 ccgctacttt gcccgccaca tcacccgcca cacctggctg tacaccgaaa tggtgaccac
     5701 tggcgcgctg atccatggcg acatcccgcg ccacctgcgc tttgacccga tggaatcgcc
     5761 cattgccctg caactgggcg gcagcgaacc ggccgacctg gcgcactgcg ccaaactcgc
     5821 agaaaactgg ggctacgacg aggtgaacct gaatgtcggc tgtccgtccg agcgggtgca
     5881 gcgcggcgcc tttggcgcct gcctgatgaa cgaagccgcg ctggtggccg actgcgtgaa
     5941 agccatgcgc gacgcggtga gcatcccggt gacggtcaag caccgcattg gcgtcgatca
     6001 gatcgagcac tatggctttc tggccgagtt tgttgacacc gtcgccgccg ccggctgcac
     6061 gacgtttatc gtgcacgccc gcaacgccat cctgaaaggc ctgtcgccca aggaaaaccg
     6121 ggaaatcccg ccgctgaaat acgattatgt gtaccggctg aaacaagaac gccccgagct
     6181 ggaaatcctg atcaacggcg gcgtgcgcac gctggaagac atcgacaccc atttgcagca
     6241 tgtggacggc gtgatgatcg gccgcgaggc ctaccacaac ccctacctga tggccgcctt
     6301 cgacgcccgc tactacggcg acgaccaccc catccccagc cgcgaagaca tcctggccgc
     6361 catcaccccg tacatcgccc gcgaactggc cgacggcaac cgcatgaaca acctcacccg
     6421 ccacatcctc ggcctctacc aaggccaacc cggcgcccga cgctttcgcc aactgctgtc
     6481 cgacagccgc cggctggcgg aaaaacgggt ggcgctgctg caagaggcgt gtccgactgc
     6541 gggcggaaaa gcatgaaaca ccaccggctc acctgcctgt cagcggcgct gaatgcacat
     6601 gcgcacgcgc catggtcttg agtctgtcgc gcttagccgg tttgccctcc cgtgtggcgc
     6661 gctgagcatc gcagaaagtc agggggcttt ccggcccggc taagcgacgc cgcaggcggc
     6721 gcgccattcc gggccggccc tgactttcga gaag
//
LOCUS       DAOPYX010000160         6749 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_13796_length_6749_cov_12.045563, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000160 DAOPYX010000000
VERSION     DAOPYX010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 6749)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6749)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6749
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13796_length_6749_cov_12.045563"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..150
                     /locus_tag="PKN55_08990"
     CDS             <1..150
                     /locus_tag="PKN55_08990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014088569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coproporphyrinogen III oxidase"
                     /protein_id="HNX71421.1"
                     /translation="LFGLQSGGRTESILMSMPPVVKWRYNWQPEAGSAEARLMSEFLV
                     EKDWV"
     gene            661..5100
                     /gene="gltB"
                     /locus_tag="PKN55_08995"
     CDS             661..5100
                     /gene="gltB"
                     /locus_tag="PKN55_08995"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014088398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HNX71422.1"
                     /translation="MERQQWLQGTLYRPDFEQDSCGFGLIAHMDDKPSHWLVKTAISS
                     LAQLTHRGAVAADGKSGDGCGLLFRKPDGFLREVAAEAGIALKSVYAAGLVFSNAQDG
                     ERVSLARLTEALTAQGLEVAGYREVPIDVSACGEYALKTLPAIRQVFVNCPFGMDETR
                     FQLGLYMGRRMAEKANADDPFFYIPTLSPHTLSYKGLVTPDNLPVFFKDLQDERFESS
                     LAVFHQRFSTNTWPQWKLAQPFRFLAHNGEINTVQGNRNWARAREQIMQSELLDMDAV
                     RPIVQTNGSDSMSLDNMLEGLLMGGVPLFRALRMLVPPAWQNVDSTDKDLRAFYEFNS
                     MHMEPWDGPAGIVLTDGRYAGCLLDRNGLRPARYVVTKDRILTISSEVGVWDYKPEDV
                     VKKGRVKPGQIVAVDLHNGELLLPEDIDAILKNAQPYRQWIRHNARHLEISLEEDSTM
                     PTMASDELARQQKLFGLSFEERDQIIRVLAEDAQEAVGSMGDDTPMAVLSQQVRSPFD
                     YLRQQFAQVTNPPIDPIREAVVMSLNTCFGPERSMFQESPEHAKRLEVRSPLLSYEKF
                     TFVVSQAESQFRAARFDLNYDPASQDLEQAIRKLTADVIAAVKDGVVIVVLSDRCIAE
                     GRLPIHALFATGAVHHALIDAGLRCRANLVIETGSARDPHQIASLVGYGATAVYPYLA
                     YQTILDMINAGLIQADPVDALRNYRKGINKGLLKVLSKMGISTIASYRGAQLFEVVGM
                     HDEVVELCLRGTVSRVSGSRFSDFEADQKKQARLAFNPMRPIAQGGLLKYVHGEEYHA
                     YNPDVVMMLQKAVQNSDYDAYQTYAHLVNTRPVAMLRDLLKLNAGNAIPLEEVEPVEA
                     ILKRFDSAGMSLGALSPEAHEALAEAMNRLGGRSNSGEGGEDPARYGTVKMSKIKQVA
                     SGRFGVTPHYLVNAEVLQIKVAQGAKPGEGGQLPGDKVSPLIAKLRCSKPGISLISPP
                     PHHDIYSIEDLAQLIFDLKQVNPTALVSVKLVAGPGVGTIAAGVAKAYADLITISGYD
                     GGTGASPLTSVKYAGTPWELGLTEAQQVLRANGLRGRVRMQTDGGLKTGLDVIKAAIL
                     GAESFGFGTGPMVALGCKFLRICHLNNCATGVATQEVKLRQKHFIGLPEMVMNYFTFI
                     ARETREWMAKLGVRSMEELIGRLDLLSIDGAVTSKQQGLNLSALLTQGNIPADEPRFC
                     VTAGNPSFDKGELAEQMVVDAEPALSCTQAQVLEYPIRNINRSIGARLSGEIARRHGA
                     DGLPDGMLKVKFTGSAGQSFGVWNAKGLALELEGDANDYVGKGMAGGSLVIYPPKNST
                     FASNEAIIVGNTCLYGATGGELYAAGLAGERFGVRNSGALAVIEGAGDHCCEYMTGGC
                     VIVLGETGYNFGAGMTGGFGFVYDREEKFAYRYNNELIDINLINGEAMGQYRAFLLDK
                     LSRHAELTGSALAAEMVSNFDDYVDYFWLVKPKAAKLDSLLKD"
     gene            5191..6600
                     /locus_tag="PKN55_09000"
     CDS             5191..6600
                     /locus_tag="PKN55_09000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014088397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNX71423.1"
                     /translation="MGDVFQFMNLQRNPGEKIAAEVRKQQFFEIYQPLNTGEAGEQAG
                     RCLGCGNPYCEWECPVHNYIPNWLKLVKEGKLFEAAELSHKTNSLPEICGRVCPQDRL
                     CEGSCTLNQGGFGAVTIGSVEKYITDEAYKAGWRPDVSKVVWTDKKVAIIGAGPAGLG
                     CADVLARNGVKPVVFDRYEEIGGLLTFGIPEFKLEKSVVKRRRELMEGMGVEFRLNTE
                     VGRDITIGQLLGEYDAVFMGMGAYQYMKGGFPGENLPGVSEALPFLINNVRNSMGTLG
                     DQPFDSAAGQRVVVLGGGDTAMDCNRTSIRQGAARVVCAYRRDEANMPGSKREVANAK
                     EEGVEFLWNRQPVEILEHNGALGVKLVTTQLGAPDAKGRRAPEVVPGSEEIIECDRVI
                     VAFGFQVKPENWFEAAGIKTDERGRTVASASQAFKHQTSNPKIFAGGDQVRGADLVVR
                     AVYEGRQAAEGMLGYLGVW"
BASE COUNT         1281 a         2086 c         2194 g         1188 t
ORIGIN      
        1 ctgttcggcc tgcaatccgg cggccgcacc gaatccatcc tgatgtcgat gccgccggtg
       61 gtgaaatggc gctacaactg gcaacccgaa gccggcagcg ccgaagcgcg gctgatgagc
      121 gaatttttgg tggagaagga ttgggtgtga gggtgaggcc cggtggccac ctctcatgac
      181 cgaatgactg atggaatgga tggcccatgt acgcatagac ggacatgggc tgtttttatg
      241 gcgcaagcac gggctgattg tctgctgctg tccggtgttg cgtgcaacgg ccacaatccg
      301 ccatcccttg tctgccactg cccttgtgcg caccgggtga tgcgcgcact atccgatagt
      361 cgaatcttgt gcagccagtt ttgcggcgga tttgtacaaa atttgctgat gccggcccgc
      421 agtgcgcaac gcgcatgatg ctgatagcgg ggcaacatcg tcgctttggc gaacaatttt
      481 ggcgcgtttt tgtacaagtt ggcggtttcc gtgcgcggca catgctgaat ggatgtttaa
      541 aatattgccc aagtgtttga ttttgctcct ataacgcgtt gcacaaattc catttgccgc
      601 atggggggtg aagtcttaca atcgaaacat tttctttatc agttctgtag gctacgaagc
      661 atggaaagac agcagtggtt gcagggcacg ctctaccgcc cggactttga acaggacagc
      721 tgcggctttg gcctgatcgc ccatatggac gacaagccca gccattggct ggtcaaaacc
      781 gccatcagct ccttggccca gttgacccac cgtggcgctg tggctgccga tggcaagtcc
      841 ggcgacggtt gcggcttgct gttccgcaag ccagacggtt ttctgcgtga ggtggcggct
      901 gaggccggca ttgcgctgaa atcggtgtac gcggccggtc tggtgttttc caacgcgcag
      961 gacggcgaac gtgtgtcgct ggcccgcctg accgaggcct tgaccgccca gggtctggaa
     1021 gtcgccggct atcgcgaggt gccgattgat gtgtccgcct gtggcgaata cgcgctcaag
     1081 accttgccgg cgattcgcca ggtgttcgtc aattgcccgt tcggcatgga cgaaacccgc
     1141 ttccagctcg gcctgtacat gggccggcgc atggcggaaa aggccaacgc cgacgatccg
     1201 ttcttctaca ttccgacgct gtcgccgcac accctgtcgt acaagggcct ggtgacgccg
     1261 gataacctgc cggtgttctt caaggacttg caggacgagc gtttcgaatc cagcctggcg
     1321 gtgttccacc agcgcttctc gaccaacacc tggccgcaat ggaagctggc gcagccgttc
     1381 cgcttcctgg cgcacaacgg tgaaatcaac accgtgcagg gcaaccgcaa ctgggcgcgc
     1441 gcgcgcgaac agatcatgca gtccgagttg ctggacatgg acgccgtgcg cccgatcgtg
     1501 cagaccaatg gctcggattc gatgagcctg gacaacatgc tggaaggcct gctgatgggc
     1561 ggcgtgccgc tgttccgcgc cttgcgcatg ctggtgccgc cggcctggca gaatgtggac
     1621 agcaccgaca aggatctgcg cgccttctac gaattcaact cgatgcacat ggagccgtgg
     1681 gacggcccgg cgggcatcgt gctgaccgat ggccgctacg ccggctgtct gctggaccgc
     1741 aacggcctgc gcccggcgcg ctatgtggtc accaaagacc gcatcctgac catctcgtct
     1801 gaagtcggcg tgtgggacta caagccggaa gacgtggtga aaaagggccg ggtcaagccg
     1861 ggccagatcg ttgctgtgga cctgcacaac ggcgagctgc tgctgccgga agacatcgac
     1921 gccatcctga aaaacgccca gccgtatcgc cagtggattc gccataacgc gcgccatctg
     1981 gaaatctcgc tggaagaaga ctccaccatg ccgaccatgg ccagcgacga gctggcccgc
     2041 cagcaaaagc tgtttggcct gagctttgaa gagcgcgacc agatcatccg cgtgctggcc
     2101 gaagacgccc aggaagcggt gggttcgatg ggcgacgaca cgccgatggc ggtgctgtcg
     2161 caacaggtgc gttcgccgtt tgactacctg cgccagcagt ttgcccaggt gaccaacccg
     2221 ccgatcgacc cgatccgcga agcggtggtg atgtcgctga acacctgctt tggcccggaa
     2281 cgcagcatgt tccaggagtc gccggaacac gccaagcgcc tggaagtgcg ctcgccgctg
     2341 ctgtcctacg agaagttcac ctttgtggtg tcgcaggctg aatcgcaatt ccgcgccgcg
     2401 cgctttgacc tgaactacga tccggccagc caggatctgg aacaggccat ccgcaagctg
     2461 accgccgacg tcatcgccgc cgtcaaggat ggcgtggtga ttgtggtgct gtctgaccgc
     2521 tgtatcgccg aaggccgcct gccgattcac gcgctgttcg ccacgggcgc cgtgcaccat
     2581 gcactgatcg acgccggcct gcgctgtcgc gccaacctgg tgatcgaaac cggctcggcc
     2641 cgcgacccgc accagatcgc cagcctggtg ggctacggcg ccaccgccgt gtacccgtac
     2701 ctggcttacc agaccattct ggacatgatc aacgccggcc tgatccaggc cgacccggtg
     2761 gacgcgctgc gcaactaccg caagggcatc aacaagggcc tgttgaaggt gctgtccaag
     2821 atggggattt ccaccatcgc cagctaccgt ggcgcccagc tgtttgaagt ggtcggcatg
     2881 cacgacgaag tggtcgagct gtgtctgcgc ggcaccgtgt cgcgcgtgtc cggctcgcgc
     2941 ttcagcgact ttgaagccga ccagaaaaaa caagcgcgtc tggccttcaa tccgatgcgc
     3001 ccgattgccc agggcggcct gctcaaatac gtccacggcg aggaatacca cgcctacaac
     3061 ccggacgtgg tgatgatgct gcaaaaggcg gtgcagaaca gcgactacga cgcctaccag
     3121 acttacgctc acctggtgaa cacccgcccg gtggccatgc tgcgcgacct gctcaagctg
     3181 aacgccggca acgccattcc gctggaagaa gtcgagccgg tggaagccat cctcaagcgc
     3241 tttgactctg ccggcatgtc gctgggcgcg ctgtcgccgg aggcgcacga agcgctggcc
     3301 gaggcgatga accgcctggg cggccgctcc aactccggtg aaggcggcga agacccggcc
     3361 cgttacggca cggtgaagat gtccaagatc aagcaggtgg cgtctggccg ttttggcgtc
     3421 accccgcact atctggtcaa cgccgaagtg ctgcaaatca aggtggccca aggcgccaag
     3481 ccgggcgaag gcggccagct gccgggcgac aaggtcagcc cgctgatcgc caagctgcgc
     3541 tgttccaagc cgggcatcag cctgatttcg ccgccgccgc accacgacat ctactcgatc
     3601 gaagatctgg cccagctgat ttttgacctg aagcaggtca acccaaccgc gctggtgtcg
     3661 gtcaagctgg tggccggccc aggcgtgggc accatcgccg ctggcgtggc caaggcctac
     3721 gccgacctga tcaccatctc gggctacgat ggcggcactg gcgcttcgcc gctgacctcg
     3781 gtcaagtacg ccggcacccc gtgggagctc ggcctgaccg aagcccagca ggtgctgcgc
     3841 gccaacggcc tgcgcggccg cgtgcgcatg cagaccgacg gcggcctgaa aaccggcctg
     3901 gacgtgatca aggccgccat cctgggcgcc gaaagctttg gctttggcac cggcccgatg
     3961 gtggcgctgg gttgcaaatt cctgcgcatc tgccacctga acaactgcgc cactggcgtg
     4021 gccacccagg aagtcaagct gcgtcagaag cacttcatcg gcttgccgga aatggtgatg
     4081 aattacttca ccttcatcgc ccgcgaaacc cgcgagtgga tggccaagct gggcgtgcgc
     4141 agcatggaag agctgattgg ccgcctggat ctgttgagca tcgacggcgc agtgaccagc
     4201 aagcagcaag gcctgaacct gagcgcgctg ctcacccagg gcaatatccc ggctgacgaa
     4261 ccgcgcttct gcgtgaccgc cggcaacccg agctttgaca agggcgagct ggccgagcaa
     4321 atggtggtgg acgccgagcc ggcgctgtcc tgcacgcaag cccaggtgct ggaatacccg
     4381 atccgcaaca tcaaccgctc gattggcgcg cgcctgtccg gcgaaatcgc ccgccgccac
     4441 ggcgccgacg gcctgccgga cggcatgctg aaggtcaagt tcaccggttc ggccggtcag
     4501 agctttggcg tgtggaacgc caagggtctg gcgctggagc tggaaggcga cgccaacgac
     4561 tacgtgggca agggcatggc cggcggttcg ctggtgatct acccgccgaa aaactcgacc
     4621 ttcgccagca acgaagccat catcgtcggc aacacctgcc tgtacggcgc cacgggcggc
     4681 gagctctacg ccgctggcct ggccggcgag cgttttggcg tgcgcaactc tggcgcgctg
     4741 gcggtgattg aaggcgcggg cgaccactgc tgcgaataca tgaccggcgg ttgtgtgatc
     4801 gtgctgggcg aaaccggcta taacttcggc gcaggcatga ccggcggctt tggcttcgtg
     4861 tacgaccgcg aagaaaaatt cgcctaccgc tacaacaacg agctgatcga catcaacctc
     4921 atcaacggcg aggcgatggg ccagtaccgc gccttcctgc tggacaagct cagccgccac
     4981 gccgagctga ccggcagcgc gctggccgcc gagatggtga gcaattttga cgattacgtg
     5041 gattacttct ggctggtcaa gccgaaggcc gccaagctcg acagcctgct caaggactga
     5101 tgacgacggg cggcgcacgc cgccgcccgg ccgacaacgc attagcgcgc cgcccgcccc
     5161 ccagctggcg cggcgtttgc gaggaacatg atgggtgacg tattccagtt tatgaatctg
     5221 cagcgcaacc ccggcgaaaa aatcgccgcc gaggtgcgca agcagcagtt ttttgaaatc
     5281 taccagccgc tcaacaccgg cgaagccggc gagcaggctg gccgctgtct gggctgcggc
     5341 aatccctact gcgagtggga atgcccggtg cacaactaca tccccaactg gctgaagctg
     5401 gtcaaggaag gcaagctgtt tgaagccgcc gagctgtcgc acaagaccaa cagcctgccg
     5461 gaaatctgcg gtcgcgtctg cccgcaagac cggctgtgcg aaggctcgtg cacgctgaac
     5521 cagggcggtt ttggcgccgt gaccatcggc tcggtggaaa aatacatcac cgacgaggcc
     5581 tacaaggctg gctggcgtcc ggacgtgtcc aaagtggtgt ggaccgacaa aaaagtcgcc
     5641 atcatcggcg ctggcccggc tggcctgggc tgtgccgacg tgctggcgcg caacggcgtc
     5701 aagccggtgg tgtttgaccg ttacgaagaa atcggcggcc tgctgacctt tggcatcccc
     5761 gagttcaagc tggaaaaatc ggtggtcaag cgccgccgcg agctgatgga aggcatgggc
     5821 gttgagttcc gcctgaacac cgaagtcggc cgcgacatca ccatcggcca gctgctgggc
     5881 gaatacgacg ccgtgttcat gggcatgggc gcctaccagt acatgaaggg cggcttcccc
     5941 ggcgaaaacc tgccgggcgt cagcgaggcg ctgcctttcc tgatcaacaa tgtgcgcaac
     6001 agcatgggca cgctgggcga ccagccgttc gacagcgcgg caggccagcg cgtggtggtg
     6061 ctgggcggcg gcgacaccgc catggactgc aaccgcacct cgattcgtca gggcgcggcg
     6121 cgcgtggtgt gcgcctaccg tcgtgacgag gccaatatgc cgggctccaa gcgcgaagtg
     6181 gccaacgcca aggaagaagg cgtcgagttc ctgtggaacc gccagccggt ggaaatcctg
     6241 gagcacaatg gcgcgctggg cgtgaagctg gtgaccaccc agctgggcgc gccggatgcc
     6301 aagggccgtc gtgcgccgga agtggtgccg ggttcggagg agatcatcga gtgcgatcgg
     6361 gtgatcgtgg cgtttggttt ccaggtgaag ccggaaaact ggtttgaagc cgccggcatc
     6421 aagaccgacg agcgcggccg cacggtggcc tcggccagcc aggcgttcaa gcaccagacc
     6481 agcaacccga agatttttgc cggcggcgat caggtgcgcg gcgcggatct ggtggtgcgc
     6541 gcggtgtatg aaggtcgtca ggcagcggaa gggatgttgg ggtatttggg ggtgtggtaa
     6601 gccgattggg ctgccagtga tgtgtgccgc gcctgcggcg cgcaggggct ggggcctgga
     6661 ttggggcggg ggttcccccc gcacccgacc ctatttcttt gtctcgccaa agaaataggg
     6721 gaaagaaagg cgacccgggc gtcgcgccc
//
LOCUS       DAOPYX010000161         6723 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_13863_length_6723_cov_12.074385, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000161 DAOPYX010000000
VERSION     DAOPYX010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 6723)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6723)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6723
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13863_length_6723_cov_12.074385"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(119..526)
                     /locus_tag="PKN55_09005"
     CDS             complement(119..526)
                     /locus_tag="PKN55_09005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003822514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HNX71424.1"
                     /translation="MLSDGWALAGLALSAFTSATILPGSSEAALLALLYAQPTLLWPA
                     LLTASLANTAGGLTSLWLGQRAPTPPKPGRALSWLHRHGAPVLALSWVPLLGDALCVA
                     AGWLRLPWWPCLLWLALGKTARYAVLVWLAKGV"
     gene            complement(516..830)
                     /gene="cyaY"
                     /locus_tag="PKN55_09010"
     CDS             complement(516..830)
                     /gene="cyaY"
                     /locus_tag="PKN55_09010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018748746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HNX71425.1"
                     /translation="MTESEFLDLSDAVFERIQTALDEAGSDVEALLQGNVLTLDCESG
                     GQVIVNRHVPNAEIWLAGKAGGFHFRLADDGRWVDTRDGGELFARLAELIQAQAGETI
                     AF"
     gene            1240..2463
                     /gene="lysA"
                     /locus_tag="PKN55_09015"
     CDS             1240..2463
                     /gene="lysA"
                     /locus_tag="PKN55_09015"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012695512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HNX71426.1"
                     /translation="MCADHTPLTAIAEQFGTPCFVYSADALSESFASYANAFAGQDHL
                     VCFALKANSNLSIIRHFARLGAGFDIVSGGELQRVLAAGGDAGRVVFSGVGKSEAEMR
                     LALEAGVLCFNVESVNELERLNQVAGAMGRRAPVSLRVNPDVDAKTHPYISTGLKDNK
                     FGIAYAEARAVYRRAAALANLDVVGIDCHIGSQLTDLTPLLDALDRLLLLVDQLAEDG
                     ITLHHLDIGGGVGIRYRDETPPDLNRYAQAVREKLADRKLKVLMEPGRSLVGNAGVLL
                     TRVEYLKLGETKHFAIVDAAMNDLMRPSLYDAWHDIVPVSQTDGPAQLVDVVGPVCES
                     SDFLGKDRQLAVEEGALLAVLSAGAYGSTMSSNYNTRARAAEVLVENGQPRLIRERDS
                     FAQMVANELNLLEPR"
     gene            2466..2951
                     /locus_tag="PKN55_09020"
     CDS             2466..2951
                     /locus_tag="PKN55_09020"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNX71427.1"
                     /translation="MPNSSLLIREARLDDPRDAESVVKLVDSYASDIIGGGKSLTPEA
                     RERLIPALIAHPSKLIFLAYQGEESVGLALCFIGFSSFQAKPLINIHDLAVLPSHRGQ
                     GIGHRLIDAVAVRGRELGCCKMTLEVRPDNEPGLALYKKAGFNRSMLGNYPYWMMDKS
                     L"
     gene            3082..3966
                     /locus_tag="PKN55_09025"
     CDS             3082..3966
                     /locus_tag="PKN55_09025"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018151963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HNX71428.1"
                     /translation="MSTVTPHDLLRTLYTMAMMVEARDAYTGGHLWRVAQFSRLLAQA
                     CGLSDREVERVALGGFLHDLGKVGVPDAVLNKPGKLTDEEYDIIKTHPRVGADLLAGH
                     PLAALAMDAVLLHHETPDGRGYPQGLSGEAIPPVARMVGIADAFDAMTSSRPYRAGMP
                     IARALDIIQDNLGSQFDRAFGQHFIALGRAGQLEAIVGHSEPGLPLQSCPGCGPIIAV
                     LREQQDGDSVLCPSCASEFQLSLHQGRRSLRPTGRQLPALARQPRANPALIESLVQAA
                     DPVLSAPRPRRWPAWLGG"
     gene            complement(3974..4606)
                     /locus_tag="PKN55_09030"
     CDS             complement(3974..4606)
                     /locus_tag="PKN55_09030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009115904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNX71429.1"
                     /translation="MKVLVIPVTSYQQNCSLIWCEHTHRAALVDPGGDVDRLLAEVEK
                     RGLTLERLLITHGHLDHAGGVAELTARLGLPVEGPHQADQFWLDGMQKQAQMFGFPPT
                     EGFTPTRWLNDGDVVTVGEASLEVLHCPGHTPGHVVFFHRGEQLAFVGDVLFQGSIGR
                     TDFPQGDYDTLINAIKGKLLPLGDEVSFVPGHGPASTLGHERRHNPYLRG"
     gene            4832..6178
                     /locus_tag="PKN55_09035"
     CDS             4832..6178
                     /locus_tag="PKN55_09035"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNX71430.1"
                     /translation="MQILFVHQNFPGQFKHLAPALAAKPEHTVVALTMRQIDSRQWQG
                     VHIVRYAPKTGSQPGVHPWLRDFDTKVIRGEACFRAALQLREQGFQPDVIIAHPGWGE
                     SLFLKQVWPQARLGLYCEFYYLLQGGDIGFDPEFPTRDADGEACRVEMRNLNNAVHMP
                     LADAAISPTHWQASTFPEPFRSRISVIHDGIDTELVAPDDSVALQLGELKLSRQDEVI
                     TFVNRNLEPCRGYHVFMRALPELLRRRPHARVLITGGDGVSYGAAPTNGQTWKQIYID
                     EAHAQHPDMDWSRVHFLGQIPYAQFVAMLQLSRVHLYLTYPFVLSWSLLEAMSAGCAI
                     VASDTAPLREAIRHDETGVLVDFFDPAALAEATCQLLDDPARRARLGAAARVFARENY
                     DLKQVCLPRQMAWVEALAARPAGEAPATAPGWTPPPPAQTTAPAPRPAVAPAFKRY"
     gene            6197..6649
                     /gene="cadR"
                     /locus_tag="PKN55_09040"
     CDS             6197..6649
                     /gene="cadR"
                     /locus_tag="PKN55_09040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009240734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cd(II)/Pb(II)-responsive transcriptional
                     regulator"
                     /protein_id="HNX71431.1"
                     /translation="MQIGELARLTGCAVDTIRYYEKQSLLPPPARSAGNFRDYADSHV
                     ERLRLIRHCRALDMSLDEIRSLLAVHDQPQADCSAVNALLDAHLGHVRARLRELQALE
                     GALVSLRQRCASAGAAAQCGILHELTHTLEALPAVGEGGSGHVHGAHG"
BASE COUNT         1189 a         2232 c         2161 g         1141 t
ORIGIN      
        1 cggcggggcg tggtgcgtaa ggcatcgtcg ctcactcatt caactgccac agacaacaca
       61 gcacaccaga cagacctggg ccgcgatctc gcatcctcaa tatagccatt tttgcccatt
      121 ataccccctt ggccagccag accagcacgg cgtagcgggc ggttttgccc agcgccagcc
      181 acagcaggca gggccaccac ggcaggcgca gccagccggc ggcgacgcac agcgcgtcgc
      241 ccagcagcgg cacccaggat agcgccagca ccggggcgcc gtggcggtgc agccagctca
      301 gcgcgcggcc gggtttgggc ggggtggggg cgcgttggcc cagccatagg ctggtgagcc
      361 cgccggcagt gttggccagg ctggcggtga gcagcgccgg ccacagcagg gtgggctggg
      421 cgtacagcag cgccagcagg gcggcttctg agctgccggg caggatggtg gcggaggtga
      481 acgccgacag cgccagcccg gccagcgccc agccgtcaga aagcaatcgt ctcgcccgct
      541 tgcgcctgga tcagctcggc cagccgggca aacaattcgc cgccatcgcg ggtgtccacc
      601 cagcggccgt cgtcggccag ccgaaagtga aaaccgccag ccttgcccgc cagccagatc
      661 tcggcattgg gcacatgccg gttgacgatc acctgcccgc ccgactcgca atccagcgtc
      721 agcacattgc cttgtagcaa ggcttccaca tccgagccgg cctcgtccag cgcggtctgg
      781 atgcgttcga acacagcgtc ggacaggtca agaaattcac tttcggtcat cggggcagtt
      841 ctccagaatc aggccgggcc tggggcaaaa tcagcaatcg tgtcgcgtga tgacgccggg
      901 cgggcaagac gcgcaagcgc cacggcagcg gggccaaacc accgacgccg gccgggcaca
      961 aagccggcgc gccccatttc atgccgcgcc gcggcatgaa ccgcaacccg aatcacggta
     1021 tcatcacgtt ttcacctgat tgtgccacag aaggcgctac tgcccggaga ccgccatgcg
     1081 aacgctctgt ctcgccattg ccctgagtct ggccctgggg gcctgtggct acaaaggccc
     1141 gctgatcaag cccgcgcccg accagacagc caagccctcc caaccttgat tgccccagga
     1201 tgccccgtga cttcgcattt tgactaccac gaccgcacgc tgtgcgccga ccacacgccg
     1261 ctgaccgcca ttgccgaaca atttggcacg ccgtgctttg tttactccgc cgacgcgctg
     1321 agcgaaagct ttgccagcta cgccaacgcc tttgccggcc aggatcacct ggtgtgcttt
     1381 gcgctgaaag ccaattccaa tctgtcgatc atccgccact ttgcccgtct gggcgcgggc
     1441 tttgacatcg tgtccggcgg cgagctgcaa cgcgtgctgg cggccggcgg cgacgcgggc
     1501 cgcgtggtgt tctccggcgt gggcaagagc gaggccgaaa tgcgcctggc gctggaagcc
     1561 ggcgtgctgt gctttaacgt cgaatcggtc aacgagctgg agcggctgaa ccaagtcgcc
     1621 ggcgccatgg gccggcgcgc gccggtcagc ctgcgcgtga acccggatgt ggacgccaaa
     1681 acccacccgt atatctccac cggcctgaaa gacaataaat tcggcatcgc ctacgccgaa
     1741 gcgcgcgcgg tgtatcgccg cgcggcggcg ctggccaatc tggacgtggt gggcatcgac
     1801 tgccatatcg gctcgcaact gaccgacctc accccgctgc tggacgcgct ggatcgcctg
     1861 ttgctgctgg tggatcaact ggccgaagac ggcatcaccc tgcaccacct ggacatcggc
     1921 ggcggcgtgg gcattcgcta ccgcgacgaa accccgcccg acctgaaccg ctacgcccag
     1981 gccgtgcgcg agaaactggc cgaccgcaag ctgaaagtgc tgatggagcc aggccgctcg
     2041 ctggtgggca atgccggcgt gctgctgacc cgcgtggaat acctcaagct gggcgaaacc
     2101 aagcactttg ccatcgtcga tgcggcgatg aacgacctga tgcgcccgtc gctgtacgac
     2161 gcctggcacg acatcgtgcc ggtgagccag accgatggcc cggcgcagct ggtggacgtg
     2221 gtcggcccgg tgtgcgagtc cagcgatttt ctgggcaagg accgccagct ggcggtggaa
     2281 gaaggcgcgc tgctggcggt attgtcggcc ggcgcctatg gttcgaccat gagctccaac
     2341 tacaacaccc gcgcccgcgc ggcggaagtg ctggtggaaa acggccagcc gcgcctgatc
     2401 cgcgagcgcg acagctttgc gcagatggtg gccaatgaac tcaatttgct ggaaccccgc
     2461 taaccatgcc taattcttcc ttgctgattc gtgaagcccg cctggacgat ccccgcgacg
     2521 ccgaaagcgt ggtcaaactg gtggacagct acgccagcga catcatcggc ggcggcaaaa
     2581 gcctgacgcc agaagcgcgc gagcgcctga tcccggcgct gatcgcccac ccgtccaagc
     2641 tgattttttt ggcctaccaa ggcgaagaat ccgtcggcct ggcgctgtgc tttattggtt
     2701 tttccagctt tcaggccaag ccgctgatca atatccacga cctggccgtg ctgcccagcc
     2761 accgtggcca gggcatcggc caccggctga tcgacgccgt ggcggtgcgc ggccgcgagc
     2821 tgggctgctg caagatgacg ctggaagtgc gcccggacaa cgaaccgggc ctggcgctgt
     2881 ataaaaaagc cggttttaac cgctcgatgt tggggaatta tccgtactgg atgatggata
     2941 aatcactgta agcgccgcgc cgatggtctt gccatgaaaa aaccctgaac gccatgcgtt
     3001 cagggttttt tcatggtggg ctgtcaggtt gctgcgccag cggcgactaa acggtattca
     3061 ctgacccagc cggagcctgc catgtccacc gtcaccccac acgatttact gcgcaccctg
     3121 tacaccatgg ccatgatggt cgaagcgcgg gatgcctata ccggcggcca tttgtggcgg
     3181 gtggcgcagt tcagccgcct gctggcccag gcttgcgggc tgtcggatcg tgaggtggag
     3241 cgggtggcgc tgggtgggtt tttgcacgat ctgggcaagg tgggcgtgcc ggatgcggtg
     3301 ctgaacaagc cgggcaagct caccgatgaa gaatacgaca tcatcaaaac ccatccacgg
     3361 gtgggcgccg acttgctggc cggccacccg ctggcggcgc tggccatgga tgccgtgctg
     3421 ctgcaccacg aaaccccgga cgggcgaggt tatccacagg gcttgagcgg tgaggcgatt
     3481 ccgccagtgg cgcgcatggt gggcattgcc gatgccttcg acgccatgac cagcagccgc
     3541 ccctaccgcg ccggcatgcc gattgcccgc gcgctggaca ttattcaaga caatctgggc
     3601 agccaattcg accgggcatt tggccagcat ttcatcgcgc tgggccgggc cggccagctg
     3661 gaggcgattg tgggccacag cgagccgggc ttgccgctgc aaagctgccc cggctgtggg
     3721 ccgatcattg ccgtgttgcg cgagcaacag gacggcgaca gtgtgctgtg cccctcctgc
     3781 gccagtgagt ttcagctctc cctgcaccag ggccgccgca gcctgcgccc gaccggccga
     3841 cagctgccgg cgctggcgcg ccagccgcgc gccaatccgg cgctgattga atcactggtg
     3901 caagcggctg atccggtgtt gtctgcgccc cggccacggc gttggccggc ctggctgggg
     3961 ggctgagtcg ggtttacccg cgcagatacg gattgtggcg gcgttcgtgg cccaaggtgg
     4021 aagccgggcc atggcccggc acaaagctga cttcgtcgcc cagcggcagc agcttgccct
     4081 tgatggcgtt aatcagcgtg tcgtaatcgc cctgggggaa gtcggtacgg ccaatcgagc
     4141 cctgaaacag cacatcgccg acaaaggcca gctgttcgcc ccgatggaaa aacaccacat
     4201 ggccaggggt gtggccgggg cagtgcagca cctccaggct tgcttcgccc accgtcacca
     4261 catcgccatc gttcagccag cgtgtcgggg taaagccctc ggtaggcgga aaaccgaaca
     4321 tctgcgcctg tttttgcatg ccatccagcc agaattgatc tgcctgatgc gggccttcca
     4381 ccggcaggcc cagtcgtgcg gtcagctcgg caacgccacc cgcgtgatcc agatgaccgt
     4441 gggtgatcag caggcgctcc agcgtcagcc cgcgtttttc tacttcggcc agcaggcgat
     4501 cgacatcacc gcccggatcc accagcgcgg cgcggtgggt gtgttcacac cagatcaggg
     4561 agcagttttg ctggtagctg gtaaccggga tgacaagcac tttcatgatg ggggcctgag
     4621 gaacataaaa aaaccaagca tagcgcaagc gccggggctt gcgtcgtcct tggcgtggca
     4681 gccaattccc tggccttgta ctggtctggc cagacgcgct gcgctgcgtg tggtgggcgg
     4741 cgcttgtggc ggtggacggt ccgccatctg ttttgcatgg aatagccgtt gtaccatacc
     4801 gttttgttcc agccacgcgc gaagcccact tatgcagatc ctgtttgttc accagaactt
     4861 tcccggccag ttcaagcatc tggcgcccgc gctggccgcc aagccggagc acaccgtggt
     4921 ggcgctgacc atgcgccaga tcgacagccg gcaatggcag ggcgtgcaca tcgtgcgcta
     4981 tgcgccaaaa accggcagcc aaccgggcgt acacccctgg ctgcgtgact ttgacaccaa
     5041 ggtgattcgt ggcgaggcgt gttttcgggc ggcgctgcaa ctgcgcgaac agggttttca
     5101 gcccgacgtg atcatcgccc atcccggctg gggcgaaagc ctgtttctca agcaagtctg
     5161 gccgcaggcg cggctggggc tgtactgcga gttttattac ctgttgcagg gcggcgatat
     5221 cggctttgac ccggaattcc ccacccgcga cgccgacggc gaggcgtgtc gggtggagat
     5281 gcgcaacctc aacaatgcgg tgcacatgcc gctggccgac gccgccatct cgcccaccca
     5341 ctggcaagcc agcacctttc ccgagccgtt ccgttcgcgc atctcggtga ttcacgatgg
     5401 catcgacacc gaactggtgg ccccggatga cagcgtggcg ctgcaactgg gcgagctcaa
     5461 gctcagccgc caggatgaag tgattacctt cgtcaaccgc aatctggaac cctgccgggg
     5521 ctaccatgtg ttcatgcgcg cgctgcccga gctgctgcgc cgccggccgc atgcccgtgt
     5581 gttgattacc ggcggtgatg gcgtcagcta tggcgctgcg ccgaccaatg gccaaacctg
     5641 gaagcaaatc tatatcgacg aagcacatgc ccagcacccg gacatggact ggtcgcgcgt
     5701 gcattttctg gggcagatcc cctatgccca gtttgtggcc atgctgcaac tgagccgcgt
     5761 ccacctgtat ctgacctacc cctttgtgct cagctggagc ctgctggaag ccatgagcgc
     5821 cggttgcgcc attgtggcca gcgacaccgc gcccttgcgc gaggccatcc gccacgacga
     5881 aaccggcgtg ctggtggact ttttcgaccc ggccgcgctg gccgaagcca cctgccaatt
     5941 gctggacgac ccagcccggc gcgcacgcct gggggcggcc gctcgcgtct ttgcccgtga
     6001 gaattacgac ctgaaacagg tctgcctgcc ccggcagatg gcttgggtgg aagcactggc
     6061 cgccaggccg gctggcgaag ccccggccac ggcgcccggc tggacgccgc caccgccggc
     6121 ccagaccacg gcgcccgcgc cacgcccggc cgtggcgccc gcgttcaaac gctactgaac
     6181 ccgctcagga gccccgatgc aaattggcga actcgcccgg ctgaccgggt gcgcggtgga
     6241 caccatccgc tattacgaaa aacagtccct gctgcccccg ccggcgcgct cggcggggaa
     6301 tttccgcgat tacgccgaca gtcatgtcga gcggctgcgc ttgattcgcc attgccgggc
     6361 gctggacatg agcctggacg aaatccgcag cctgctggcg gtgcacgacc agccgcaggc
     6421 cgattgcagc gcggtgaacg ccctgctgga cgcgcacctg ggccatgtgc gcgcgcggct
     6481 gcgcgaattg caggcgctgg aaggcgcgct ggtcagcctg cggcaacgct gcgccagcgc
     6541 cggggcggca gcgcagtgcg gcattttgca cgagctgacc cacacgctgg aggccttgcc
     6601 cgcagtgggc gaaggcggca gcggccatgt gcacggcgcg catggctaag agtcgctcac
     6661 aaaactttcc cgccgcgcaa cgcccgccag ccaccttctg aacatatggc gaatgcgtct
     6721 gag
//
LOCUS       DAOPYX010000162         6694 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_13950_length_6694_cov_15.147462, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000162 DAOPYX010000000
VERSION     DAOPYX010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 6694)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6694)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6694
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13950_length_6694_cov_15.147462"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(4..2724)
                     /gene="ppc"
                     /locus_tag="PKN55_09045"
     CDS             complement(4..2724)
                     /gene="ppc"
                     /locus_tag="PKN55_09045"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014085557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HNX71432.1"
                     /translation="MNETEKDLPLRHDLARLDRLLAEIVREQEGEAVYHEMRTIPVEA
                     HDRVGTPELSRRLAELSPAAAAALVRACGFYAQLTNLAEDLHHNRRRRAHRAAGSAPQ
                     QGSLALAIQRLKHDAVPFAALVDFFEHAQISPVLTAHPTEVQRQSVLDAHKAIRKFLT
                     KLADPSLGREDREEIEAKLKRVILALWQTSEIRPFKLTVKDEIENGVAYHPTTFFTAL
                     PKLYASLEDAISREWGQRLTLPNFFRIGSWIGGDRDGNPFVTADVTLYAAQRQAEVAF
                     AWYGRELDQLYRELSLSARRVKVSEALTALASHSPDHTESRQEEPYRRALATLQARLT
                     ATAQALDVPFDGRFANTGATVYHSPTELAHDLQVIADSLCAHGSQTLAAGRLRDVIRA
                     VEVFGFSLMPLDMRQFSGIFEAVVAELFSQAGAGDYLAADEAGRQQMLLAELQSPRLL
                     FSPFVDYSEQTSKELALFNAAAQIQTRFGKKALPNCIISNCASASDLLEVALLLKEAG
                     LLRVVDGEPTATVNIIPLFETVADLDASAGVMSELFALPWYRTLLKSRKDTQEVMLGY
                     SDSNKDGGYFTSQWELYQAERRLVKVFDAAGVRMRLFHGRGGSVGRGGGPSYEAIMAQ
                     PAGSVAGQIRITEQGEVIGAKYSDPEIGLRNLEALLAATLEASLSTVHDTPPAEEATL
                     GELSQRAFHAYRSLVETPGFIQYFLEATPINEIAKLNIGSRPASRKSLTSIKDLRAIP
                     WVFSWAQARVMLPGWYGVGSAVAAYVEEHGDAGRDRLRALYRNSAFFQVALSNMEQVL
                     AKSDLQIAAGYAALVSDQSLAKHIFGLIEAEWRRTRDAFTLITGQADLLADNPSLARS
                     LANRLPFMDALNILQIELIRRLRQTPDAADLLYTAHQTINGIAAGLRNSG"
     gene            2792..3712
                     /gene="hemC"
                     /locus_tag="PKN55_09050"
     CDS             2792..3712
                     /gene="hemC"
                     /locus_tag="PKN55_09050"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HNX71433.1"
                     /translation="MKQLVIASRESALAMWQAEHIQARLQALYPGLSVSILGMTTQGD
                     QILDKTLSKIGGKGLFVKELEVALQEGRADLAVHSMKDVPMNLPDGFELAAICTREDP
                     RDAFVSNRYAALADLPDGAVVGTSSLRRESQLRARYPHLTIRPLRGNVQTRLRKLDDG
                     EFDAIILAAAGLKRLGLSERIRIELAPQESLPAVGQGALGIEILEGRAELAALLAPLN
                     DAVTAACVRAERAMSRALNGSCQVPLGGYAVQRGDMLTLNGFVASVDGQTFLTARADA
                     PARYADALGRAVAKQLLDKGADDIIAALDL"
     gene            3709..4485
                     /locus_tag="PKN55_09055"
     CDS             3709..4485
                     /locus_tag="PKN55_09055"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008954627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HNX71434.1"
                     /translation="MSQVQPLAGRRVVVTRPAGQAGALMAKLAAAGATPIALPVIDIA
                     PADAATLRAAADVTLAADRAIFVSPTAIELLWPHLAGRLGARPALATVGAASARRLGE
                     LSQRPVLHPQGRSDADALAALPELAAPLHGQRIALVKGSGGRSPRLAAQLSERGAEVT
                     MLDVYRRQPASPDWHHVDAALAGPGVDAVALTSSEAVDWLFMLGGESRRATLQSLLYV
                     APHPRIAQRLAECGASRCLITAADNDALVAALCEWFETRP"
     gene            4482..5498
                     /locus_tag="PKN55_09060"
     CDS             4482..5498
                     /locus_tag="PKN55_09060"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: protein motif:HMM:NF016275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HNX71435.1"
                     /translation="MTTSSPYTPPRPARKLNVALLLALAALATAGGLWLDSRASLDAL
                     QQTLTQKAGAASGVSKDVQARAEHADKTARENSARLAALEGKVGDFAGQAAALNSLYQ
                     EQSRNREDWMLAEIEHALNLAGQQLQLAGNVAAASSTLEAIDARLAKLDRPQFIPLRK
                     ALGQDLGQLKSAPAVDVVGLTVKLDRLAAEIDALPLVIDHARQQADATPAPSHQGVPA
                     WQRLGEQLLDDIGQLIRVRRMDKPEALLLSPEQAFLLRENVKLRLLGARVAVLQRQDA
                     PFRTDLSASERYVRGYFDTQAPAVGVWLAALNEIKTTQLSVRVPDISASLRAVRALQG
                     RPQE"
     gene            5502..>6694
                     /locus_tag="PKN55_09065"
     CDS             5502..>6694
                     /locus_tag="PKN55_09065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011133605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme biosynthesis HemY N-terminal
                     domain-containing protein"
                     /protein_id="HNX71436.1"
                     /translation="MKVVLWIVGLFALAVGLSLFAQINTGYAIVFVPPYRVELSLNMT
                     LLLAVGLIAVSYGLLRLLAATLNLPHEVAVFQRRKKLRIARHALVQSSLAYSEGRFQK
                     AEREALRACENEQSPENQGVALLLAARAAHAMRAYDRRDGYLARMDSLGNGLQLARRM
                     LEAELLLDEKRTVDALAAVEAARAQSPNLTAALRLELKIRQLQSSPEHVLALTEKLLK
                     SEAISAEQARRYRLAAYRDQLPQLIDGQELRQWWQRIPAQERQNPSLALAVAQQYAKL
                     EQADDAAHLLQGLLEDDYSSELVSELGELADQVSEARRIELLRLGEALLPSHSRDAQL
                     LLTLGRLARAGKLWGKAQSYFEASLSIQPTLAAHAELARLLQALDRADEARRHFDACL
                     ELALA"
BASE COUNT         1096 a         2249 c         2288 g         1061 t
ORIGIN      
        1 cgcttagccg ctgttgcgca ggccggcggc aatgccgttg atggtctggt gcgcggtgta
       61 cagcaaatcg gcggcgtccg gcgtctggcg caggcggcgg atcagctcga tttgtagaat
      121 gttcagcgca tccatgaatg gcaggcggtt ggccaggctg cgcgccagcg aggggttgtc
      181 ggccagcagg tctgcttgcc cggtgatcag ggtgaaggcg tcgcgggtgc gtcgccattc
      241 ggcttcgatc aggccaaaga tgtgcttggc caggctctgg tcggacacca gcgcggcgta
      301 gccggcggca atctgcaaat cgctcttggc cagcacctgc tccatattgg acagcgccac
      361 ctggaaaaac gccgaattgc ggtacagcgc gcgcaggcgg tcgcggccgg cgtcgccgtg
      421 ttcttccaca taggcggcca ctgcgctgcc cacgccgtac cagcccggca gcatcacccg
      481 cgcctgcgcc caggagaaca cccaggggat ggcgcgcagg tctttgatgc tggtcaggct
      541 cttgcgcgag gccgggcggc tgccgatatt gagcttggcg atttcattga tcggcgtggc
      601 ttccaggaag tactgaataa agcccggcgt ttccaccagc gagcggtagg cgtggaaggc
      661 gcgctgcgac aattcgccca gcgtggcttc ttcggccggc ggggtgtcgt gcaccgtgct
      721 caggctggct tccagcgtgg cggccagcag ggcttccagg ttgcgcaggc cgatttccgg
      781 gtcggaatac ttggcgccaa tcacctcgcc ttgttcggtg atgcggattt gcccggccac
      841 cgagccggcc ggctgcgcca tgatggcctc gtaagacggg ccgccgccac ggcccaccga
      901 gccgccgcga ccatggaaca ggcgcatgcg cacgccagcg gcgtcgaaca ccttcaccag
      961 gcggcgctcg gcctggtaca gctcccactg gctggtgaag taaccgccgt ccttattgct
     1021 gtccgaatag cccagcatca cctcttgcgt gtccttgcgg ctcttgagca gggtgcgata
     1081 ccacggcagg gcgaacagct cgctcatcac cccggcgctg gcgtccaggt cggcgacggt
     1141 ttcaaacagc ggaatgatat tgacggtggc ggtcggctcg ccgtccacca cgcgcagcag
     1201 gccggcttct ttcagcagca gcgccacttc cagcaggtcg ctggcgctgg cgcagttgga
     1261 gataatgcaa ttgggcaggg cttttttgcc aaagcgcgtc tgaatctgcg cggcggcgtt
     1321 gaacagcgcc agctccttgc tggtttgctc gctgtagtcg acaaagggcg agaacagcag
     1381 acgcggcgat tgcagctcgg ccagcagcat ctgctggcgg ccggcttcgt cggcggccag
     1441 ataatccccg gcgccggcct ggctgaacaa ctcggccacc acggcttcaa aaatgcccga
     1501 gaactgacgc atgtccagcg gcatcagcga gaagccaaac acctcgaccg cgcgaatcac
     1561 gtcgcgcagg cggccggcgg ccagcgtctg gctgccgtgt gcgcacagcg agtcggcaat
     1621 cacctgcaaa tcgtgcgcca gctcggtggg cgagtggtac accgtggcgc cggtattggc
     1681 aaagcggccg tcaaacggca cgtccagcgc ctgggcggtg gcggtcagcc gcgcttgcag
     1741 ggtggccagc gcgcggcggt agggctcttc ctggcggctt tcggtgtggt cggggctgtg
     1801 gctggccagc gcggtcagcg cctcgctcac cttcacccgg cgcgccgaca gcgacaattc
     1861 gcggtacagc tggtccagct cgcggccata ccaggcaaac gccacttccg cctgacgctg
     1921 ggcggcgtac agggtgacgt cggcggtgac aaacgggttg ccgtcgcggt cgccgccaat
     1981 ccagctgcca atgcggaaga aattcggcag cgtcagccgc tggccccatt cgcgcgagat
     2041 ggcgtcttcc aggctggcgt acagcttggg cagcgcagta aagaacgtgg tcgggtggta
     2101 ggccacgccg ttttcgattt cgtctttgac ggtgagcttg aacgggcgga tttccgaggt
     2161 ttgccacagc gccagaatca cccgcttgag cttggcctcg atttcttcgc ggtcttcgcg
     2221 ccccagcgac ggatcggcca gtttggtgag aaacttgcgg atggccttgt gtgcgtccag
     2281 cacgctttgg cgctgcacct cggtggggtg ggcggtcagc accggcgaaa tttgcgcgtg
     2341 ctcaaaaaaa tccaccagcg cggcaaacgg cacggcgtcg tgcttcaggc gctgaatcgc
     2401 cagcgccagg ctgccttgct gcggggccga accggcggcg cggtgggcgc ggcgacggcg
     2461 gttgtggtgc aggtcttcgg ccagattggt aagctgtgca taaaaaccac aggcgcgcac
     2521 cagcgcggcg gcggcggcgg gcgacagctc ggccagccgg cgggataatt ccggcgtgcc
     2581 gacgcgatcg tgggcctcca ccggaatggt gcgcatctcg tgatagaccg cttcgccctc
     2641 ttgttcacgg acgatctcgg caagcaggcg gtccagtcgc gccagatcgt ggcgcagcgg
     2701 caagtctttt tcagtttcgt tcatcatggc gggataccgt gttcaaaagc gaagcggtta
     2761 caattctgtc aaattacatt aggaaagcac catgaagcaa cttgtcattg ccagccggga
     2821 gagcgcgctg gccatgtggc aggcggaaca catccaggcg cggctgcaag ccttgtatcc
     2881 gggcctgagc gtgagcatcc tgggcatgac cacccagggc gaccagattc tggacaaaac
     2941 attatcgaaa atcggcggta aagggctgtt cgttaaagaa ctggaagtcg ccctgcaaga
     3001 aggccgcgcc gatctggcgg tgcattccat gaaagacgtg ccgatgaatc tgcccgacgg
     3061 tttcgagctg gcggccatct gcacccgcga agacccgcgc gatgcgtttg tctccaaccg
     3121 ctacgccgcg ctggcggatt tgcccgacgg cgccgtggtg ggcacctcca gcctgcgccg
     3181 ggaaagccag ctgcgcgcgc gctacccgca cctgaccatc cgcccgctgc gcggcaatgt
     3241 gcaaacccgg ctgcgcaagc tggacgacgg cgaattcgac gccatcatcc tggcggcggc
     3301 gggcctcaag cgcctgggct tgtccgagcg catccgcatc gagctggccc cgcaagaaag
     3361 cctgccggcg gtgggccagg gcgcgctggg cattgagatt ctggaaggcc gcgccgagct
     3421 ggccgcgctg ctggcgccgc tgaacgacgc cgtcaccgcc gcctgcgtgc gcgccgaacg
     3481 cgccatgagc cgcgcgctga atggctcctg ccaggtgccg ctgggcggct acgccgtgca
     3541 gcgcggcgac atgctcaccc tgaacggctt tgtcgccagc gtggacggcc agaccttcct
     3601 caccgcccgc gccgacgccc cggcgcgcta tgccgacgcg ctgggccgcg ccgtggccaa
     3661 gcagctgctg gacaagggcg cggacgacat catcgccgcg ctggatttgt gagccaggtc
     3721 cagccgctgg ctggccggcg cgtggtggtc acccgcccgg ccggccaggc cggcgcgctg
     3781 atggccaagc tggccgccgc cggcgccacg ccgattgccc tgccggtgat cgacatcgcg
     3841 ccggccgacg ccgccaccct gcgcgcggcg gccgatgtca ccctggcggc ggatcgggcg
     3901 atttttgtgt cgcccaccgc catcgagctg ctgtggccgc atctggccgg ccggctgggc
     3961 gcgcgcccgg cgctggccac ggtgggcgcc gccagcgcgc gccggctggg cgaactgagc
     4021 cagcggccgg tgttgcaccc gcaagggcgc agcgacgccg acgcgctggc ggcgctgccg
     4081 gaactggccg cgcccttgca cggtcaacgc attgccctgg tgaagggcag cggcgggcgc
     4141 agcccgcgcc tggccgcgca attaagcgaa cgcggcgccg aggtgaccat gctggacgtc
     4201 taccgccgcc agccggccag cccggattgg catcatgtgg acgccgccct ggccggcccc
     4261 ggcgtggatg ccgtggcgct gaccagctcg gaagcggtgg actggttatt catgctgggt
     4321 ggcgagtcgc gccgggcaac gttacaatcc ttgctctacg tggcgcctca cccgcgcatt
     4381 gcccagcgcc tggccgaatg cggcgcctcg cgctgtctga ttaccgcagc ggataacgac
     4441 gccctcgtcg ccgcgctttg cgaatggttt gaaacccgcc catgactaca agctcgccgt
     4501 acacccctcc ccgcccggcc cgcaaactga acgtcgccct gctgctggcg ctggccgccc
     4561 tggccacggc cggcggcctg tggctggaca gccgcgcctc gctggacgct ttgcaacaga
     4621 cgctgacgca aaaagccggc gccgccagcg gcgtgagcaa ggacgtgcag gcgcgcgccg
     4681 agcacgccga caaaaccgcc cgcgaaaaca gcgcgcggct ggccgcgctg gaaggcaagg
     4741 tgggcgattt tgccggccag gccgccgcgc tgaacagcct gtaccaagag cagtcgcgca
     4801 accgcgaaga ctggatgctg gccgaaatcg aacacgcctt gaatctggcc gggcagcaat
     4861 tgcagctggc cggcaatgtc gccgccgcca gcagcacgct ggaagccatc gacgcccggc
     4921 tggccaagct ggaccgcccg cagttcatcc cgctgcgcaa ggccctgggc caggatctgg
     4981 gccagctgaa atccgccccg gcggtggacg tggtgggcct gaccgtcaag ctggatcgcc
     5041 tggccgccga gatcgacgcg ctgcccttgg tgatcgacca cgcccgccag caggcggacg
     5101 ccacgcccgc gcccagccac cagggcgtgc cggcctggca gcgcctgggc gagcaattgc
     5161 tggacgacat cggccagctg attcgcgtgc ggcgcatgga caaacccgaa gcgctgctgc
     5221 tgtcgcccga gcaggcgttt ttgctgcgcg aaaacgtcaa gctgcgcctg ctgggcgcgc
     5281 gcgtggcggt gttgcagcgc caggatgcgc cattccgcac cgacctgagc gccagcgaac
     5341 gctatgtgcg cgggtatttt gacacccagg cgccggcagt gggcgtctgg ctggccgcgc
     5401 tcaatgaaat caagaccacc cagctgtccg tgcgcgtgcc cgacatcagc gccagcctgc
     5461 gggcggtgcg cgccttgcag ggccgtcccc aggagtaagt gatgaaagtt gtgctgtgga
     5521 ttgtcggcct gttcgccctg gcggtgggtc tgtccttgtt tgcccagatc aacaccggct
     5581 acgccatcgt gtttgtgccg ccctaccggg tggagctgtc gctgaacatg accctgctgc
     5641 tggcggtggg cctgattgcc gtgtcgtatg gcctgctgcg cctgctggcc gccacgctga
     5701 acctgccgca tgaagtggcg gtgttccagc gccgcaaaaa gctgcgcatt gcccgccacg
     5761 cgctggtgca atcgtcgctg gcgtactcgg aaggccgctt ccagaaggcc gagcgcgagg
     5821 cgctgcgcgc ctgcgaaaac gaacaaagcc cggaaaacca aggcgtggcg ctgctgctgg
     5881 cggcgcgcgc cgcccacgcc atgcgcgcct acgaccgccg cgatggctat ctggcgcgca
     5941 tggacagcct gggcaatggc ctgcaactgg cccgccgcat gctggaagcc gagctgctgc
     6001 tggacgaaaa acgcacggtg gatgcgctgg cggcggtgga agccgcgcgc gcgcaatcgc
     6061 ccaacctcac cgccgcgctg cgcctggagc tgaaaatccg ccagctgcaa agctcgcccg
     6121 agcatgtgct ggcgctgacg gaaaaactgc tcaaatccga agccatcagc gccgaacaag
     6181 cgcgccgcta ccggctggcc gcctaccgcg accagctgcc gcagctgatc gacggccagg
     6241 agctgcgcca gtggtggcag cgcattccgg cgcaagagcg gcaaaacccc agcctggcgc
     6301 tggcggtggc gcagcaatac gccaagctgg agcaagccga cgacgccgcc cacctgctgc
     6361 aaggtctgtt ggaagacgac tacagcagcg agctggtcag cgagctgggc gagctggccg
     6421 accaagtcag cgaggcgcgc cgcatcgaac tgctgcgcct gggcgaggcg ctgctgccca
     6481 gccacagccg cgacgcccag ctgttgctga ccctgggccg tctggcccgc gccggcaagc
     6541 tgtggggcaa ggcgcaaagc tattttgagg ccagcctgtc catccagccc accctggccg
     6601 cccacgccga actggcgcgg ctattgcagg cgctggaccg cgccgacgaa gcgcgccggc
     6661 actttgacgc ctgcctggaa ctggcgctgg cgga
//
LOCUS       DAOPYX010000163         6677 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_14009_length_6677_cov_8.224706, whole genome shotgun sequence.
ACCESSION   DAOPYX010000163 DAOPYX010000000
VERSION     DAOPYX010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 6677)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6677)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6677
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14009_length_6677_cov_8.224706"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(196..759)
                     /gene="ssb"
                     /locus_tag="PKN55_09070"
     CDS             complement(196..759)
                     /gene="ssb"
                     /locus_tag="PKN55_09070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012696954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HNX71437.1"
                     /translation="MASVNKVILVGNLGRDPEVRYMPNGDAVANYSIATTETWKDKNG
                     MRQEKTEWHNIVMYRRLAEIAGEYLKKGSSVYIEGRLQTRKWQDKQGNDRYTTEIVAD
                     QMQMLGGRNSGGGGGGYEGGSHDGGGYGGGGNMGGGNRGGNYGAPQGGYDDNQPPRMR
                     DDTPPPPRRQEAKPAANFDDMDDDIPF"
     gene            complement(761..2119)
                     /locus_tag="PKN55_09075"
     CDS             complement(761..2119)
                     /locus_tag="PKN55_09075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018748659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNX71438.1"
                     /translation="MTKLELRASLGLAGIYALRMLGMFLILPVFALYAHQLPGGDNPT
                     WIGLALGSYGLTQALLQLPLGMLSDRIGRKKVIYGGLAVFALGSFVAAGAHDLLTLTL
                     GRTLQGAGAISAAVTALLADLTREEHRTRAMAMIGMTIGLTFAASLVLGPLLARLIGV
                     PGIFALTGALSLLALVWVKFAIPDPSVSRFHSDAEANTARLPAVLRDGQLLRLNFGIF
                     ALHAAQMAMFVVIPFALIHHGLDKAHHWQVYLPVVVAGFVLMVPAIIYGEKRHQLKKV
                     FVAAIALMAAAQLGMTLALDSLTAIVVLLGGYFIAFNILEATLPSLISKIAPADAKGT
                     AIGVYNTAQSLGLFLGAAAGGWLYSHGGAPFVFAFCSALMLLWLLMAITMRPPRAVRT
                     QMLALPDGWQGNPATLSARLRALHGVEEAVVIAAERVAYLKVAQTGWDEAGASQSIRE
                     TQ"
     gene            2357..5188
                     /gene="uvrA"
                     /locus_tag="PKN55_09080"
     CDS             2357..5188
                     /gene="uvrA"
                     /locus_tag="PKN55_09080"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012697077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HNX71439.1"
                     /translation="MDLIRIRGARTHNLKNVNLDLPRHKLIVMTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLQLMEKPDVDLIEGLSPAISIEQKATSHNPRSTVGTVT
                     EIHDYLRLLFARVGTPFCPEHEQPLESQTVSQMVDHVLALPADTKLMVLAPVVVGKKG
                     EQLDLFDELRAQGFVRVRVDGEVYELDAVPKLDKNKKHTVDVVVDRLKVREDAKQRLA
                     ESFETALRHADGRAIALEMDSGQEHWFSAKFACPVCSYSLPELEPRLFSFNNPMGACP
                     KCDGLGQISFFDPKRVVAHPELSLAAGAVKGWDKRNQFYFQMLQSLAMHYGFDIDTPF
                     ENLPEAVRKVVLYGSGSESIPFIYLNEKGSKFTRSHPFEGILHNFERRYRETDSSAVR
                     EELVKYQNSQPCPSCGGSRLRREARHVRVGGETLHTINQWPLKQTAGFFSSLAFSGQK
                     QAVAEKIVKEISDRVQFLVNVGLDYLSLDRSADTLSGGEAQRIRLASQIGSGLTGVMY
                     VLDEPSIGLHQRDNDRLLATLMRLRDLGNSVIVVEHDEDAIRAADYVVDMGPGAGEHG
                     GEVLVAGTPAEVAAHGDSVTGAFLSGRRQIAVPAQRRTPDPARWLTLSGARGNNLKNV
                     TLQLPLGLLVCITGVSGSGKSTLINDTLYHAVARHLHGGSAEPAPFDDIQGLEQFDKV
                     INVDQSPIGRTPRSNPATYTGLFTPIRELFAGVPMARERGYGPGRFSFNVKGGRCEAC
                     QGDGMIKVEMHFLPDIYVPCDVCHGKRYNRETLEVQYKGKSIHEVLDMTVEHALEFFA
                     AVPTVARKLQTLMDVGLGYIRLGQSATTLSGGEAQRVKLALELSKRDTGRTLYILDEP
                     TTGLHFHDIDLLLQVLHRLREHGNTIVVIEHNLDVIKTADWIIDLGPEGGAGGGQILA
                     AGAPEEVAVHPDSHTGHYLKKILDAASGR"
     gene            complement(5310..5915)
                     /gene="recR"
                     /locus_tag="PKN55_09085"
     CDS             complement(5310..5915)
                     /gene="recR"
                     /locus_tag="PKN55_09085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012696448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HNX71440.1"
                     /translation="MKNPPSLDQLISALRVLPGVGPKSAQRMAYHLLQRDKPGAEKLA
                     RALEHALSHIGHCQRCNTFSETPLCALCADPARRQDQLCVVEMPADMLTLEHTQSYDG
                     LYFVLMGRLSPLDGVGPRDLPLEKLAHRALDGAVREVILATNFTAEGETTAHALSELL
                     KGHGLTLTRMARGLPVGGELEHTDPGTLAQALFERRGVTGG"
     gene            complement(6000..6326)
                     /locus_tag="PKN55_09090"
     CDS             complement(6000..6326)
                     /locus_tag="PKN55_09090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008954482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HNX71441.1"
                     /translation="MFNKGGIAGLMKQAQQMQENMKKAQEQLATIEVEGQSGAGMVKV
                     VMTCQHDIKRVSIDDSVMDDKEMLEDLVAAAVNDAVRKVESTTQEKMSGFTAGLNLPP
                     GLKLPF"
     gene            complement(6333..>6677)
                     /locus_tag="PKN55_09095"
     CDS             complement(6333..>6677)
                     /locus_tag="PKN55_09095"
                     /inference="COORDINATES: protein motif:HMM:NF023592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau C-terminal
                     domain-containing protein"
                     /protein_id="HNX71442.1"
                     /translation="LGAKEIMLAQNAELTGVRGNLFCLRAPPQFRFSATPEVQALLAQ
                     ALSEALGAPVQLEVALGEISGETPFMQQQRLRGEALEAARERLQQDPVVTALVREFDA
                     TLRPDSIQPVQE"
BASE COUNT         1256 a         2054 c         2149 g         1218 t
ORIGIN      
        1 tactgcttac tgcttacatc tcgtctaact taaatcagat tgtactgtcc tgaaagcagc
       61 taaacatgtt gacgcgcgca aagactgata cataaaaata tgttatggcg atttataaaa
      121 aatgtaaaca ataaaaaagc ccaaagtaac gagtactttg ggctttttta ttgtttacga
      181 gctcaaaaaa tcgactcaaa acggaatatc atcatccata tcatcaaaat tcgctgccgg
      241 cttggcttcc tggcggcgcg gcggcggtgg ggtgtcgtcg cgcatgcgcg gcggttggtt
      301 gtcgtcgtag ccgccttgcg gagcgccgta gttgccgccg cgattgccgc cacccatatt
      361 gccgccgccg ccgtagccgc cgccgtcatg gctgccgcct tcgtagccgc cgccaccgcc
      421 gccgctgttg cggccaccca gcatttgcat ttggtcggcg acgatttcgg tggtgtagcg
      481 gtcgttgcct tgcttgtctt gccatttgcg ggtttgcagg cggccttcga tgtacaccga
      541 gctgcctttt ttcaggtatt caccggcgat ttccgccaga cggcggtaca tgacaatatt
      601 gtgccattcg gttttttcct ggcgcatgcc atttttgtct ttccaggttt ccgtggtggc
      661 gatgctgtag ttcgccacgg cgtcgccgtt gggcatgtag cgcacttccg ggtcgcggcc
      721 gaggttgccg accagaatca ccttgttgac cgatgccatt ttattgagtc tccctgattg
      781 attggctggc gcctgcttcg tcccagccgg tttgcgccac cttgaggtag gccacgcgct
      841 cggcggcaat caccaccgct tcttccacgc catgcagggc gcgcaggcgg gccgacagcg
      901 tggccgggtt gccctgccag ccgtcgggca gcgccagcat ctgggtgcgc acggcgcgcg
      961 gcgggcgcat ggtgatggcc atgagcagcc acagcagcat caaggcggag cagaatgcaa
     1021 acacgaacgg ggcgccgcca tggctgtaca accagccccc ggcagccgcc ccgagaaaca
     1081 ggcccagcga ttgggcggta ttatacacac cgatggcggt gcccttggcg tcggcggggg
     1141 cgattttgga aatcagcgag ggcagtgtcg cttccaggat gttgaaggcg atgaaatacc
     1201 cccccagcaa gaccacaatg gcggtcagcg aatccagcgc cagggtcatg cccagctgcg
     1261 cggcggccat cagggcgatg gcggcgacaa acactttttt cagttggtgg cgtttttcgc
     1321 catagataat ggccggcacc atcagcacaa agccggccac caccaccggc agatacacct
     1381 gccagtggtg cgccttgtcc agtccgtggt gaatcagggc aaaggggatg accacaaaca
     1441 tggccatttg cgcggcgtgc agggcaaaga tgccaaaatt cagccgcagc agttggccat
     1501 cgcgcagcac ggcgggcagc cgggcggtgt tggcttcggc atcggaatga aagcgcgaca
     1561 cgctggggtc ggggatggcg aatttcaccc acaccagcgc cagcaggctg agcgcgccag
     1621 tcagggcaaa aatccccggc acgccaatca gccgcgccag cagcgggccc agcaccaggc
     1681 tggcggcaaa ggtcaggcca atggtcatgc caatcatcgc catggcgcgg gtgcggtgtt
     1741 cttcgcgggt caggtcggcc aacagcgcgg tgacggcggc ggaaatcgcc ccggcgcctt
     1801 gcagggtgcg gcccagggtg agggtgagca gatcatgcgc gccagcggcc acaaagctgc
     1861 ccagggcaaa caccgccagc ccgccgtaaa tcaccttttt gcggccgatg cggtcggaca
     1921 gcatgcccag cggcagttgc agcagggctt gcgtcagccc gtagctgccc agcgccaggc
     1981 caatccaggt ggggttgtcg ccgccgggca gctgatgggc gtacagggca aacacaggca
     2041 ggataagaaa catgcccagc atgcgcaggg cgtagatgcc ggcaagcccc aggcttgccc
     2101 gtagttcgag tttggtcatg gtgacagctg tatgctgggc gcacggtggg ggtgggccat
     2161 ccgttgcgcc ggttcaggtt ccgagggggg ccgccatctg cccgccgcaa tgttgcgcca
     2221 cggcaaaatt cgcggcacac agtgtgccag cgcgggggag cgccagcttg cagtgcggca
     2281 agtcagcagg cttgggccaa tggcgggaaa cacggtatat taaccgattc ctccccggct
     2341 ttcagcgcag acactcatgg atctgatccg cattcgcggt gcacgcacgc acaacctcaa
     2401 aaacgtcaat ctggatttgc cccgacacaa gctgattgtg atgacgggcc tgtccggctc
     2461 gggcaagtct tcgctggcgt ttgacaccct gtacgccgag ggtcagcgcc gctatgtgga
     2521 aagcctgtcc gcctacgccc ggcagttttt gcagctgatg gaaaaacccg acgtagacct
     2581 gatcgaaggc ttgtcgccag cgatttccat cgagcaaaaa gccaccagcc acaacccgcg
     2641 ctcgacggtg ggcacggtga ctgaaatcca cgattatctg cgcctgttgt ttgcccgcgt
     2701 cggcacgccg ttttgccccg agcacgagca gccgctggaa agccagacgg tcagccagat
     2761 ggtggaccat gtgctggcct tgccggccga caccaagctg atggtgctgg cgccagtggt
     2821 ggtgggcaaa aagggcgagc agctggattt gtttgacgag ctgcgcgcgc agggctttgt
     2881 gcgcgtgcgg gtggacggcg aggtttacga gctggacgcc gtgcccaagc tggacaaaaa
     2941 caaaaagcac actgtcgatg tggtggttga ccggctgaaa gtgcgcgaag acgccaagca
     3001 acggctggcg gaatctttcg aaaccgcgct gcgccacgcc gacggccggg cgattgcgct
     3061 ggaaatggac agcggccagg agcactggtt ttccgccaag tttgcctgcc cggtgtgcag
     3121 ctacagcctg cccgagctgg agccgcgcct gttttcgttc aataacccga tgggcgcctg
     3181 ccccaagtgc gatggcctgg ggcaaatcag cttttttgac cccaagcgcg tggtggccca
     3241 ccccgagctg agcctggccg ctggcgcggt gaagggctgg gacaagcgca accagtttta
     3301 ttttcagatg ctgcaaagcc tggccatgca ttacggcttt gacatcgaca ccccgttcga
     3361 aaacctgcca gaagcagtgc gcaaggtggt gttgtatggc tcgggcagcg aatccatccc
     3421 gtttatttac ctgaacgaaa aaggcagcaa attcacccgc agccatccgt ttgaggggat
     3481 tttgcacaat ttcgagcgcc gctaccggga aaccgactcc agcgcggtgc gcgaagaatt
     3541 ggtcaaatac caaaacagcc agccctgccc cagctgcggc ggctcgcgcc tgcgccgcga
     3601 ggcgcgccat gtgcgggtgg gcggcgaaac cctgcacacc atcaaccaat ggccgctcaa
     3661 gcaaaccgcc gggtttttca gctcgctggc gttcagcggg caaaagcagg cagtggcaga
     3721 aaaaatcgtc aaggaaatca gcgaccgcgt gcagtttctg gtgaacgtcg gcctggatta
     3781 cctcagcctg gaccgctcgg ccgacaccct gtccggcggc gaagcgcagc gcatccgcct
     3841 ggccagccag atcggctctg gcctgaccgg ggtgatgtat gtgctggacg agccgtccat
     3901 cggcctgcac cagcgcgaca acgaccgcct gctggccacg ctgatgcggc tgcgcgatct
     3961 gggcaatagc gtgattgtgg tcgaacacga cgaagacgcc atccgcgccg cggattacgt
     4021 ggtggacatg ggccccggcg ctggcgagca cggcggcgag gtgctggtgg ccggcacgcc
     4081 ggccgaagtg gccgcgcatg gagattcggt caccggggcg tttttgtctg gccgccggca
     4141 aatcgccgtg ccggcccagc gccgcacgcc cgacccggcg cgctggctga cgctgagcgg
     4201 cgcgcgcggc aacaacctga aaaacgtcac cctgcaactg ccgctgggct tgttggtgtg
     4261 catcaccggg gtgtctggct cgggcaaatc cacgctgatc aacgacacgc tataccacgc
     4321 cgtcgcccgc catctgcacg gcggcagcgc cgagccggcg ccgtttgacg acatccaagg
     4381 tctggagcag ttcgacaagg tgatcaacgt cgatcaaagc ccgattggcc gcaccccgcg
     4441 ctccaacccg gccacctaca ccgggctgtt caccccgatt cgcgagctgt ttgccggcgt
     4501 gccgatggcg cgcgagcgcg gctacgggcc ggggcgcttt tccttcaacg tcaagggcgg
     4561 gcgctgcgaa gcctgccagg gcgatggcat gatcaaggtg gaaatgcact ttctgccgga
     4621 tatctatgtg ccgtgcgacg tctgccacgg caagcgctac aaccgggaaa ccctggaagt
     4681 gcaatacaag ggcaagagca ttcacgaagt gctggacatg accgtcgagc acgcgctgga
     4741 attctttgcc gccgtgccca cggtggcgcg caagctgcaa acgctgatgg acgtcggcct
     4801 gggctatatc cgcctggggc aaagcgccac caccttgtct ggcggcgagg cgcagcgggt
     4861 gaagctggcg ctggaattgt ccaagcgcga caccggccgc acgctgtaca tcctggacga
     4921 acccaccacc ggcctgcatt ttcatgacat tgatttgctg ttgcaggtgc tgcaccgcct
     4981 gcgcgagcac ggcaacacca ttgtggtgat cgaacacaat ctggacgtga tcaaaaccgc
     5041 cgactggatt attgacctgg gcccggaagg cggcgcgggc ggcgggcaga ttctggcggc
     5101 tggcgcaccg gaagaggtgg cggtacaccc ggatagccat accgggcact atctgaaaaa
     5161 aattctggac gcagcgtccg ggcgctgaga gtgagcgcta acagacggca agcaccagga
     5221 tacatactca caaagcacag cgaaatggcg cacggcaagc agtacggcaa ggccatgccc
     5281 tcatgccaga cgggtttggc aagcggcgcc tagccgcccg tcaccccgcg ccgctcaaac
     5341 agcgcctgcg ccagtgtgcc agggtcggtg tgctccagct cgccgcccac cggcaggccg
     5401 cgcgccatgc gcgtcagcgt caggccgtgg cccttgagca gctcggacaa ggcatgcgcg
     5461 gtggtttcgc cctcggcggt gaaattggtg gccagaatca cctcgcgcac cgcgccatcc
     5521 agcgcgcgat gggcgagttt ctccagcggc aaatcgcgcg ggccaacgcc atccagcggc
     5581 gacagccggc ccatcagcac aaaatacagg ccatcgtaac tttgcgtgtg ctccagggtg
     5641 agcatgtcgg cgggcatttc caccacacag agttgatcct gacggcgcgc cgggtcggcg
     5701 cacagcgcgc acagcggggt ttcgctgaag gtgttgcagc gttggcaatg gccgatgtgg
     5761 ctgagcgcat gttccagcgc gcgggcgagt ttttctgcgc cgggcttgtc gcgctgcaat
     5821 aggtgatagg ccatgcgctg ggcggatttg gggccaacgc cgggcagcac gcgcaaggcg
     5881 ctgatcagtt ggtcaagact gggcgggttt ttcatggggc atccggcggg gttccggcaa
     5941 acaggcagcc gcccccgcgc acgatgagcg cacgcggggg cggcaggcca gggcggggat
     6001 cagaacggca gtttcaggcc cggcggcagg ttcagaccgg cggtgaagcc ggacattttt
     6061 tcttgcgtgg tgctttcgac tttgcgcacg gcgtcattca ctgcggcggc gaccaggtct
     6121 tccagcattt ctttgtcgtc catcacgctg tcgtcgatgc tgacgcgctt gatgtcgtgt
     6181 tggcaggtca tcaccacttt gaccatgcca gccccggatt ggccctcgac ctcgatggtc
     6241 gccagctgtt cctgggcttt tttcatgttt tcctgcattt gttgggcttg cttcatcagc
     6301 ccggcgatac cgcctttatt gaacatggtg tgtcactcct gaacgggttg aatagagtcg
     6361 gggcgcaggg tggcgtcaaa ttcgcgcacc agggccgtca cgaccgggtc ttgttgcagc
     6421 cgctcgcgcg ccgcctccag ggcttcgccg cgcaggcgct gctgctgcat gaatggggtt
     6481 tcgccgctga tctcgcccag cgccacttcc agctgcacgg gggcgcccag cgcctcgctc
     6541 agcgcttgcg ccaggagcgc ttgcacctcg ggcgtggcgc taaagcggaa ctgcggcggc
     6601 gcgcgcaggc agaacagatt gccgcgcacg ccggtcagct cggcgttttg cgccagcatg
     6661 atttctttgg cgcccag
//
LOCUS       DAOPYX010000164         6577 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_14298_length_6577_cov_9.191352, whole genome shotgun sequence.
ACCESSION   DAOPYX010000164 DAOPYX010000000
VERSION     DAOPYX010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 6577)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6577)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6577
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14298_length_6577_cov_9.191352"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..639
                     /locus_tag="PKN55_09100"
     CDS             <1..639
                     /locus_tag="PKN55_09100"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011134018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNX71443.1"
                     /translation="SLLNPDKHGPVYAEFVEGAAAHRRQFGGGRGQPVAKAIGMKGQA
                     SRDVVDATAGLGRDAFVLASLGCAVRLVERSPVAAALLADALTRAHAHPDTADIAARM
                     TVIHADASDWLASLAPEARPQVVFVDPMFPDTGNKAAAKKDMQAFQEVIGGDDDSPRL
                     LAAALAAARERVVVKRPRLGADVAGPPPTSRQVGKSTRFDVYSVKAMPAGPC"
     gene            734..988
                     /gene="grxC"
                     /locus_tag="PKN55_09105"
     CDS             734..988
                     /gene="grxC"
                     /locus_tag="PKN55_09105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008954847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HNX71444.1"
                     /translation="MKPVTLYGTGVCPYCDMAERLLARKGVTPDKIRVDLEPEQREHM
                     MRITGRRTVPQIFIGELHVGGYDDLSALDRAGKLDALLAD"
     gene            1078..1524
                     /gene="secB"
                     /locus_tag="PKN55_09110"
     CDS             1078..1524
                     /gene="secB"
                     /locus_tag="PKN55_09110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008954848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HNX71445.1"
                     /translation="MSEQDLQPVFSIEKIYVKDLSLEIPHAPQIFLEREQPEIDMQLA
                     TEGKQLEDGVFEAVITVTVTAKLGEKVMFLVEAAQAGIFQIRGVPEEEISGVLAVACP
                     NILFPYVRETVSDVVNRAGFPPVLLSPINFEALYAQQMAEQSGNAH"
     gene            1514..1954
                     /locus_tag="PKN55_09115"
     CDS             1514..1954
                     /locus_tag="PKN55_09115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012698282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HNX71446.1"
                     /translation="MRTKLVLVLGLLLAAASAQALEFRSVKSHGAIMHEAPWASSKKL
                     YVLSQGYPVEVIGSQPDWVRVRDAAGGVAWMSTASLSNQRTVVATANRAELRRSPDAK
                     AALAFSVDKDVVLQMVEPPKNGWVKLRHADGSSGYGQISAFWGL"
     gene            1961..2968
                     /locus_tag="PKN55_09120"
     CDS             1961..2968
                     /locus_tag="PKN55_09120"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017508186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HNX71447.1"
                     /translation="MKLSIFGAGAWGTALAVALAREHEVTLWARDAALIEALAASRRN
                     TRYLPEAELPANLALTADFAAAAHSGELALVVTPIAGLRATLRRLHEVCGQALPPVLW
                     ACKGFEAGSGLLPHEVAAQELPADAVCGALSGPSFAQEVAAGLPSAIALALNHGGEAR
                     RLARALHTRCLRIYANQDLVGVEVGGAVKNVMAIAAGVADGLHCGHNARAALLTRGLA
                     EISRLAQALGGQAATLMGLSGLGDLILTCTGNLSRNRRVGMLLAEGRSLDDVLASLGH
                     VAEGVPTARETLARARALGVDMPITAGVCAMLFGQASPQQVVDTLLERAPKAEDGSEL
                     G"
     gene            complement(3038..3409)
                     /locus_tag="PKN55_09125"
     CDS             complement(3038..3409)
                     /locus_tag="PKN55_09125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX71448.1"
                     /translation="MYEIWLGVNILYELAWMYAPLVCGVTIMLALGYGLAWQRGGQWR
                     AALTRALTVASVTGLVSVLLLPTLTRSSLAEMGYWIDWLNLLLISAGVGVLCGLLVWP
                     FAAMRQAGVCPLGKGRCGGAH"
     gene            complement(3446..4792)
                     /locus_tag="PKN55_09130"
     CDS             complement(3446..4792)
                     /locus_tag="PKN55_09130"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HNX71449.1"
                     /translation="MRAQQMAPPPMPANPGRRELLRGLGGTALLAGIPLASPARAAPL
                     RTSARIVIVGSGLGGLAVANRLSRELDGASLTVIDAKRVHNYQPGYTLVATGIWPVEK
                     VVDDNARFMPAKVNWIRQAVVAFEPERNQVLTQDGQRVPYDFLVVATGLQLDYGQIAG
                     MDVRAIGQNGMASVYDSPEAAAATWQAMDRFRQKGGRAVMTLPHTPLKCAGAPLKMTF
                     MVDDRLREAGTRGQSSVVFYSALKDSIFSVKTVNDEVLRRWQTLDVPVNFQHRLSAVD
                     LAARKATFSRDGADPLTVEYDFLHIAPPMRAPDAVKNSPLAIQEGPLAAGGWLDVDKG
                     TLQHRRYPNVFGIGDINGTARGKTAATIKKSAPLVVHNLLRHIAGEAPDWQFDGYTSC
                     PLLIREGSALLVEFDYEGRLTPSLPFIEPLQDSYLAWLLKYRMLKPAYLSVLKGRV"
     assembly_gap    5193..5292
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5293..6554)
                     /locus_tag="PKN55_09135"
     CDS             complement(<5293..6554)
                     /locus_tag="PKN55_09135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014088446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HNX71450.1"
                     /translation="MTTWLDSVAGIVGPRHLITDPDTLAAACLDWRGRYRGRAQAIAR
                     PGSTAEVAALVRACVAAGAPIVPQGGNTSHCGAATPDDSGRALIIQLHRMQALRAVDA
                     DNNTLTAEAGCTLAQVQDAAAGVGRLFPLSLASEGSCQIGGNLATNAGGVAVLRYGPM
                     RELTLGLEAVLPDGSVLDSLSGLRKNNTGYDLKQLLIGAEGTLGIITAATLKLFPAPE
                     ATATALVEVSSPADALALLHALQARFGDRLTAFELISGLCLQLLARHLPDARPPFMPP
                     WCVLLELNDGGDEAVLNQALAEALCALRPQADAVLARHARERAQLWSLREQISEAQKR
                     DGPSIKHDIAVPLSQLAGFIERADAALQQAFPACRVVCFGHVGDGNLHYNVSFTRPGN
                     VDLFDDEPAVNALVYDLVYAHGGTLSAE"
BASE COUNT         1082 a         2168 c         2186 g         1041 t
ORIGIN      
        1 agcctgctga acccggacaa acacgggccg gtgtacgccg aatttgtcga gggcgcggcg
       61 gcgcatcgcc ggcagtttgg cggcgggcgc ggccagccgg tggccaaggc catcggcatg
      121 aaggggcagg ccagccggga cgtggtggac gccaccgccg gcctgggccg cgacgccttt
      181 gtgctggcca gcctgggctg cgccgtgcgc ctggtcgagc gctcgccagt ggccgccgcg
      241 ctgctggccg acgcgctgac gcgcgcgcat gcccacccgg acaccgccga cattgccgcg
      301 cgcatgacgg tgattcacgc cgacgccagc gactggctgg ccagcctggc gcccgaggcg
      361 cgcccgcagg tggtgtttgt ggatccgatg ttccccgaca ccggcaataa ggccgccgcc
      421 aaaaaagaca tgcaggcgtt tcaggaggtg attggcggcg atgacgacag cccgcgcctg
      481 ctggccgccg cgctggccgc cgcgcgcgag cgggtggtgg tcaaacgccc gcgtctgggc
      541 gccgatgtgg ccggcccgcc gcccaccagc cggcaggtgg gcaagagcac gcgctttgat
      601 gtgtatagcg tcaaggccat gcctgctggc ccgtgctaac gcgcgcatgt cttggatgac
      661 gttctacttg ccgctacact gactgctttt gccacgcggg ccatgccaat gcccgccccg
      721 acaggatatt gcaatgaaac ccgtcacttt gtatggcacc ggcgtgtgcc cgtactgcga
      781 catggccgag cgcctgctgg cgcgcaaggg cgtcaccccc gataaaatcc gtgtggacct
      841 tgagccagaa cagcgcgagc acatgatgcg catcaccggc cggcgcaccg tgccgcagat
      901 tttcatcggc gagctgcacg tgggcggcta cgacgacctc tcggcgctgg accgcgccgg
      961 caagctggac gccctgctgg ccgactgagc cggcgcgcgc cgtcagcgtt ggcgcggcgc
     1021 gcggcttcgt gcgatgatgc ctgactttac cgacccttct tttgaattga gagcgcgatg
     1081 agcgaacaag acctgcaacc ggtgttttcc atcgaaaaaa tctacgtcaa ggacctgtcg
     1141 ctggaaatcc cgcacgctcc gcaaatcttc ctggagcgtg agcagccgga aatcgacatg
     1201 cagctggcca ccgaaggcaa gcagctggaa gacggcgtgt ttgaagccgt gatcaccgtc
     1261 accgtcaccg ccaagctggg cgaaaaagtg atgttcctgg tcgaagccgc ccaggctggc
     1321 atcttccaga tccgtggcgt gccggaagaa gaaatcagcg gcgtgctggc cgtggcctgc
     1381 ccgaacatcc tgttcccgta cgtgcgcgaa accgtgtccg acgtggtcaa ccgcgccggc
     1441 ttcccgccgg tgctgctgtc gccgatcaat ttcgaggcgc tgtatgccca gcaaatggcc
     1501 gagcaatccg gcaatgcgca ctaagctcgt tttggtcctg ggcctgctgc tggccgccgc
     1561 cagcgcgcag gcgctggaat tccgctcggt gaagtcgcac ggcgccatca tgcacgaggc
     1621 gccgtgggcc agctcgaaaa agctctacgt gctcagccag ggctatccgg tggaagtcat
     1681 cggcagccag ccggactggg tgcgcgtgcg cgacgccgct ggcggcgtgg cctggatgtc
     1741 caccgccagc ctgagcaatc agcgcaccgt ggtggccacc gccaaccgcg ccgagctgcg
     1801 ccgcagcccg gacgccaagg cggccttggc tttctcagtg gataaggacg tggtgttgca
     1861 gatggtcgag ccgccgaaaa acggctgggt caagctgcgc cacgccgatg gcagcagtgg
     1921 ctatggccag atttcggcgt tctgggggct gtaagcagtc atgaagctgt ccatttttgg
     1981 cgctggcgcc tggggcaccg ccctggcggt ggccttggcg cgtgagcacg aggtcaccct
     2041 gtgggcgcgc gacgccgccc tgatcgaggc gctggccgcc agccggcgca acacccgcta
     2101 cctgcccgaa gccgaactgc cggccaatct ggcgctgacc gccgactttg ccgccgccgc
     2161 acactcgggc gagctggcgc tggtggtgac gccgattgcc ggcctgcgcg ccacgctgcg
     2221 ccgcttgcac gaggtgtgcg gccaggcctt gccgcccgtg ctgtgggcgt gcaagggctt
     2281 tgaggccggc agcggcctgc tgccgcacga agtggccgcg caagaattgc cggccgatgc
     2341 ggtgtgcggc gcgctgtctg gccccagctt tgcccaggaa gtggccgccg gcctgccctc
     2401 ggccattgcc ctggcgctga atcacggcgg cgaggcgcgc cggctggcgc gcgcgctgca
     2461 cacccgctgc ctgcgcattt acgccaatca ggatttggtg ggcgtggaag tcggcggcgc
     2521 ggtgaaaaac gtcatggcca tcgccgctgg cgtggctgat ggcctgcact gcggccacaa
     2581 tgcccgcgcc gcgctgctga cccgtggcct ggcggaaatc agccggctgg cgcaggcgct
     2641 gggcggccaa gccgccacgc tgatgggttt gtccggcctg ggagatttaa tcctcacctg
     2701 caccggcaat ctgtcgcgca accgccgcgt tggcatgctg ctggccgaag gccgctcgct
     2761 ggacgacgtg ctggcctcgc tgggccatgt cgccgaaggc gtgcccaccg cgcgggaaac
     2821 cctggcgcgc gcgcgcgcgc tgggggtgga catgccgatc accgccggcg tgtgcgcgat
     2881 gctgttcggg caggccagtc cgcaacaggt ggtggacacg ctgctggaac gcgcgcccaa
     2941 ggccgaggac ggcagcgagc tgggctgaaa caccgcgcac cacatgatga acgcccctga
     3001 gccaatgggg gtcagcggcg ttcccgcgag gcggtgctca gtgcgccccg ccgcagcgcc
     3061 ccttgcccag cgggcagaca cccgcctgac gcatggcggc aaacggccac accagcaagc
     3121 cgcacagcac cccgacgccg gcgctgatga gcagcaggtt cagccagtcg atccagtagc
     3181 ccatctccgc cagcgacgag cgggtcagtg tgggcagcag cagcaccgac accaggcccg
     3241 tcacgctggc cactgtcagt gcgcgcgtca gcgccgcccg ccactggcca ccgcgctgcc
     3301 aggccaggcc atagcccaac gccagcatga ttgtcacccc acacaccagc ggcgcataca
     3361 tccacgccag ttcatacaag atattcacgc ccagccagat ttcatacatg gttcgactcc
     3421 cggatcgatc gcattgccat gaagttcaca ctcgcccctt gagcaccgac agataagccg
     3481 gtttgagcat gcggtatttg agcagccagg ccagatagct gtcctgcaac ggttcgatga
     3541 agggcagcga gggcgtcagc cggccttcgt aatcgaactc caccagcagc gccgagcctt
     3601 cgcgaatcag cagcgggcag gaggtatagc cgtcgaattg ccagtctggc gcctccccgg
     3661 caatgtgccg cagcaggtta tgcaccacca gcggcgcact tttcttgatg gtggcggcgg
     3721 tcttgccacg cgcggtgccg ttgatgtcgc caatgccaaa cacattgggg tagcggcgat
     3781 gttgcagcgt gcccttgtcg acatccagcc agccgcctgc cgccagcggg ccttcctgaa
     3841 tcgccagcgg gctgtttttc accgcgtccg gtgcacgcat cggcggggcg atgtgcagga
     3901 agtcgtattc cacggtcagc gggtcagcgc catcgcggct gaaggtggcc ttgcgcgcgg
     3961 ccagatccac ggcgctgagg cgatgctgga aattgaccgg cacatccagc gtttgccagc
     4021 ggcgcagcac ctcgtcgttg acggtcttga cgctgaagat gctgtctttc agcgccgagt
     4081 aaaacaccac gctggattgc ccgcgcgtgc cggcctcgcg caggcggtca tccaccatga
     4141 aggtcatctt cagcggcgcc ccggcgcatt tgagcggtgt gtgcggcagg gtcatcaccg
     4201 cacgcccccc tttttgccgg aagcgatcca tggcctgcca agttgccgcc gccgcttcag
     4261 ggctgtcgta cacgctggcc atgccattct ggccaatggc gcgcacatcc atgccggcaa
     4321 tctggccgta atccagctgc aggccggtag ccaccaccag aaaatcgtag ggcacccgct
     4381 gtccatcctg ggtgagcacc tgattgcgct ctggttcaaa tgcaaccacg gcctgccgaa
     4441 tccagttgac ctttgccggc atgaacctgg cgttgtcatc aaccaccttt tccaccggcc
     4501 agatgccagt cgccaccagg gtgtagcctg gctgatagtt gtgcacgcgc ttggcgtcga
     4561 tcaccgtcag gctggcgcca tccagttcgc gtgacaggcg gttggctacc gccaacccgc
     4621 ccagaccgct gccgacaatg acaatgcgcg ccgaggtgcg caacggtgct gcccgggcgg
     4681 ggctggccag tggaatgccg gccagcaagg cggtgccgcc caggccgcgc aacagctcgc
     4741 gtcggccggg attggctggc atcgggggtg gggccatctg ctgtgctcgc atggtctgtc
     4801 tccttggtgt ggggatgagg cgcaacccgg ccttggtcac ggcatccccg taggtatact
     4861 taattatatt tttttatata ttaataaagt caaggagaat cgtttgcgca cagcaacatc
     4921 gaacatgctg gcaacggcag gagacaaccg gaaaaccgcc agaaaaatac cagggcgtca
     4981 ggaaacggga agggaaatat cccgtgcagg atccggcact gatggcgccc tgcgggcgac
     5041 cgtagccacc gggtgagcgc atcgcccgag ggcgacatga cggagtcaga atgtcgtgga
     5101 cgctgcgcag gccagcgaat ccgtgcacag tgccgacacc cccagcgccc acagcggcgc
     5161 ggcctgcgcc gggtcgttca ccgtccagct cannnnnnnn nnnnnnnnnn nnnnnnnnnn
     5221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5281 nnnnnnnnnn nntgctcagc cgacagcgtg ccgccgtggg cgtacaccag gtcgtacacc
     5341 agggcgttga cggcgggttc gtcgtcaaac agatcgacat tgcccggccg ggtgaacgac
     5401 acgttgtaat gcagattgcc gtcgcccaca tggccaaagc acaccacccg gcaagcgggg
     5461 aaggcctgtt gcagggcggc gtcggcgcgc tcaataaaac cggccagttg cgacagcggc
     5521 acggcaatgt cgtgcttgat gctggggccg tcgcgttttt gcgcttcgga aatctgctcg
     5581 cgcaggctcc acagctgggc gcgttcgcgg gcgtggcggg ccagcacggc gtcggcctgc
     5641 gggcgcagtg cgcacagcgc ttcggccagc gcctggttca gcaccgcttc gtcgccgccg
     5701 tcgttcagct ccagcagcac gcaccacggc ggcatgaacg gcgggcgggc gtcgggcagg
     5761 tggcgggcca gcagttgcag gcacagcccg gagatcagct caaaggcggt cagccggtcg
     5821 ccaaagcgcg cttgcagcgc atgcagcagc gccagcgcgt cggccgggct gctcacctcc
     5881 accagcgcgg tggcggtggc ttccggggcg gggaatagct tgagggtggc ggcggtgatg
     5941 atgcccagcg tgccttcggc gccaatcagc agctgtttga ggtcgtagcc ggtgttgttc
     6001 ttgcgcaggc cagacaggct atccagcacg ctgccgtcgg gcagcaccgc ttccaggccc
     6061 agggtcagct cgcgcatcgg cccatagcgc agcaccgcca cgccgccggc attggtggcc
     6121 agattgccgc caatctggca gctgccttcc gaggccagcg acagcggaaa cagccggccc
     6181 accccggcgg cggcgtcctg cacctgcgcc agcgtgcagc cggcttcggc ggtcagggtg
     6241 ttgttgtcgg cgtccaccgc gcgcagcgcc tgcatgcggt gcagctggat aatcagcgcg
     6301 cggccgctgt cgtccggggt ggcggcgccg cagtgcgagg tattaccgcc ctgcggcacg
     6361 attggcgcgc cagcggccac gcaggcgcgc accagcgcgg ccacttcggc ggtgctgccg
     6421 gggcgggcga tggcctgggc gcggccacgg tagcgcccgc gccaatccag acaggcggcg
     6481 gccagggtgt cggggtcggt gatcaaatgg cgggggccga caatccccgc cacgctgtct
     6541 agccaagtgg tcatggccgg attatcgccc agaacgc
//
LOCUS       DAOPYX010000165         6572 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_14311_length_6572_cov_11.538438, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000165 DAOPYX010000000
VERSION     DAOPYX010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 6572)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6572)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6572
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14311_length_6572_cov_11.538438"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            158..895
                     /locus_tag="PKN55_09140"
     CDS             158..895
                     /locus_tag="PKN55_09140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014085691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036361 - racemase activity, acting on
                     amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HNX71451.1"
                     /translation="MHIRLINPNTSAAMTASIGAAAQRIAAPATRLTAVNPADGPESI
                     ESHFDDAIAAVGVCEEILRGERDGVDAYIIACFGDPGLNAARELTRAPVIGIAQAAFH
                     MASLVSTRFSVVTTLRRTVILAEHLLSQYGCATLCASVRATDIPVLALEHNPSACAAQ
                     LIDQAQHARDHDGAGAIVLGCGGMAGFAADMQAALGIPVIDGVSAAVKLAEAAVGLGL
                     HTSKHGDLATPLPKSFTGRFAHFSRSA"
     gene            898..1839
                     /gene="puuE"
                     /locus_tag="PKN55_09145"
     CDS             898..1839
                     /gene="puuE"
                     /locus_tag="PKN55_09145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008954794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase PuuE"
                     /protein_id="HNX71452.1"
                     /translation="MTPLSADYPRDLRGYGRTPPHAQWPGQARIAVQFVLNYEEGGEN
                     CVLHGDAGSEQFLSEMFNPASYPERHLSMESIYEYGSRAGVWRILREFERRGWPLTVF
                     AVGMAMQRHPELAAACVELGHEIACHGWRWIHYQHMDEATEREHMRLALDTLTALTGQ
                     RPLGWYTGRDSPNTRRLVVDDGGLLYDSDYYGDDLPFWTEVTRSDGVTAPHLVVPYTL
                     DVNDMRFALPQGFSHGEPFYQYLKDSFDVLYAEGDEAPKMLSVGMHCRLLGRPGRFAA
                     LRRFMDYIASHERVWVCRRVDIARHWCEVHPYAPAAR"
     gene            1896..2927
                     /gene="alc"
                     /locus_tag="PKN55_09150"
     CDS             1896..2927
                     /gene="alc"
                     /locus_tag="PKN55_09150"
                     /EC_number="3.5.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014085696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004037 - allantoicase activity [Evidence
                     IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoicase"
                     /protein_id="HNX71453.1"
                     /translation="MSHPVSAAVRVAAAVDLPDWARRAVNLADARLGARALGASDEFF
                     APLARMLNPEPAVFVPGQYDAHGKWMDGWETRRRRGPGHDWSIIGLARRGRVFGFDVD
                     TSHFTGNYAPAIAIEATDCPSDDPEVLAHASWTPLLTSTSLRGDSHHLLAVVDDGVWS
                     HLRVHIYPDGGVARLRVYGRPAEITAGPDGLTDLAAQINGGRALAWNDAHFGQAANLL
                     APGRGVNMGDGWETRRRREPGHDWCIIELGAPGVIERIEVDTAHFKGNYPDRFSMQAA
                     RVDGGTEQSVITQSMFWPTLIGETPLSMDAEHVFSTGIAALGAVSHVRFNIHPDGGVS
                     RLRLWGRPA"
     gene            2924..3418
                     /locus_tag="PKN55_09155"
     CDS             2924..3418
                     /locus_tag="PKN55_09155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007114993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HNX71454.1"
                     /translation="MITLTASALSADAFAPFGQVLSAASAARQFTINGGNTERFHDLA
                     ELSAGADGRLIVSLFRGQPRALPFAVSMLERHPLGSQAFLPLGERPYLVVVAPPGAPP
                     SPASVRAFLAAPGQGVNYAPGVWHHPLLALDAVSDFWVIDRAGPGDNCEEITLAEPVW
                     VQRP"
     gene            3660..3938
                     /locus_tag="PKN55_09160"
     CDS             3660..3938
                     /locus_tag="PKN55_09160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008953027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit IV family protein"
                     /protein_id="HNX71455.1"
                     /translation="MLSSHSPAPTPRPVWPVWLYLSALTLALAALAEWRSHAALAALA
                     LGGAWLKGVVIIESFMALRDAPMWLRAAVHGWLALLCGGLCASFLYGG"
     gene            3941..4729
                     /locus_tag="PKN55_09165"
     CDS             3941..4729
                     /locus_tag="PKN55_09165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008953026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbbQ/NirQ/NorQ/GpvN family protein"
                     /protein_id="HNX71456.1"
                     /translation="MAATDIPFYQPAGDECEVFAHAWRQQLPVLIKGPTGCGKTRFVA
                     HMAAQLGLPLFTVSCHDDLSAADLVGRHLIAGGDTRWCDGPLTQAVRAGGLCYLDEVV
                     EARKDTTVVLHPLTDDRRMLPIERTGEVLQAPPGFMLVVSYNPGYQHVLKTLKPSTRQ
                     RFVALSFDFPRPEVECEVVRHEGGVDAELAWRLVRLGGQLRQLGGHDLDEGASTRLLV
                     YAARLMASGMPARRACEVALAEPLSDEPDTLAALRAVIDTQFGS"
     gene            4733..6568
                     /locus_tag="PKN55_09170"
     CDS             4733..6568
                     /locus_tag="PKN55_09170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014086631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase"
                     /protein_id="HNX71457.1"
                     /translation="MEDWVGGWWDRLIRRAAYRGHPQAAVALADIERQAPVFFRAMGG
                     DPALTVRSTVGAAHGARRRLLERVAGIGERVELGWRDAQSLYLPERIDLYPDAALNRE
                     LYFWLMALAARDDAHHGGDWFARNRAATAACLRQMPGLAAVYARLAAALLPLRDLPRR
                     ADERAQELAIRAALQQPHQAIALPPCAQPPQPVPLWLHPSPPGGGQAHSARRDGDATA
                     EDEASQRRDSKARRRYRARDGETDERKNGMLMVFRAESLFTWDEYVRVNRHTDEDDDS
                     DDGAAAEDMQTLTVQRDTRRRASRLKFDLDLPAAAYDDTPLGPGILLPEWDYKRQRLV
                     ADHARIQPMLPRDAAPAALPERLRPLARRLRRQFQALAPERTRKQGEASGPDIDLDRL
                     VRFLAERASGAAVAEPPLYQAWPPGGRSLACLLLADISLSTESWLGDAGQVIQVIRDS
                     LLLFAEALTACGDAFGLYGFSSIRREEVRYLLLKDFAERYDDACRGRILALRPGYYTR
                     LGAAIRHSSRILLEQAAQRRVLLILSDGKPNDLDHYEGRYGVEDTRQAVLEARRAGLI
                     PFCVTIDRQAGDYLPHLFGRHGYTVAHDARRLPQVLTRLYAGLTG"
BASE COUNT          998 a         2393 c         2166 g         1015 t
ORIGIN      
        1 tggggtcgcc tttctttgcc tactttcttt ggcgaagcaa agaaagtagg tcggccgcgg
       61 gacgaactcc cgcttaaatc agcccgcgcg cagcgcggca cactaaaacc cgcgctaagc
      121 accgccaaac cacctatacc tacccccgga ccccaccatg cacatccgcc tgatcaaccc
      181 caacacctcg gccgccatga ccgccagcat cggcgccgcc gcccaacgca tcgccgcacc
      241 agccacccgc ctcaccgccg tcaaccccgc cgacggcccc gaatccatcg aaagccactt
      301 cgacgacgcc atcgccgccg tcggcgtctg cgaagaaatc ctgcgcggcg aacgcgacgg
      361 cgtagacgcc tacatcatcg cctgctttgg cgaccccggc ctgaacgccg cccgcgaact
      421 cacccgcgcc cccgtcatcg gcatcgccca agccgccttc cacatggcca gcctggtcag
      481 cacccgcttt tccgtggtca ccaccctgcg ccgcaccgtg atcctggccg aacatttgct
      541 cagccaatac ggctgcgcca cgctgtgcgc cagcgtgcgc gccaccgaca tcccggtgct
      601 ggcactcgaa cacaacccca gcgcctgcgc cgcccaactg atcgaccaag cccaacacgc
      661 ccgcgaccac gacggcgccg gcgccatcgt gctgggctgt ggcggcatgg ccggctttgc
      721 cgccgacatg caagccgcgc tgggcatccc ggtgatcgac ggcgtcagcg cggcagtcaa
      781 actggccgaa gccgccgtcg gcctcggcct gcacaccagc aagcacggcg acctggccac
      841 gccgctgccc aaatcattca ccggccgttt tgcgcatttt tcccggagcg cctgaccatg
      901 accccactgt ctgctgacta cccccgcgac ctgcgcggct acggccgcac cccgccacac
      961 gcccaatggc cgggccaggc gcgcattgcc gtgcaatttg tgctcaatta cgaagaaggc
     1021 ggcgaaaact gcgtgctgca cggcgacgcc ggcagcgagc aattcctgtc ggaaatgttc
     1081 aacccggcca gctaccccga gcggcatttg tcgatggaat ccatctacga atacggctcg
     1141 cgcgccgggg tgtggcgcat cctgcgcgaa ttcgagcgcc gtggctggcc gctgaccgtg
     1201 tttgccgtcg gcatggccat gcagcgccac cccgagctgg ccgccgcctg cgtggagctg
     1261 ggccatgaaa tcgcctgcca cggctggcgc tggattcact accagcacat ggacgaagcc
     1321 accgagcgcg aacacatgcg cctggcgctg gacaccctca ccgcgctgac cggccagcgc
     1381 ccgctgggct ggtacaccgg ccgcgacagc cccaacaccc gccggctggt ggtggacgac
     1441 ggcggcctgc tgtacgactc ggactactac ggcgacgacc tgccgttctg gaccgaagtc
     1501 acccgcagcg acggcgtgac cgcgccacac ctggtggtgc cctacaccct ggacgtcaac
     1561 gacatgcgct ttgccctgcc gcagggcttt agccacggcg agccgtttta tcaatacctg
     1621 aaagacagct ttgacgtgct gtacgccgaa ggcgacgaag cgccaaaaat gctgtcggtg
     1681 ggcatgcact gccgcctgct cggccgcccc ggccgctttg ccgcgctgcg ccgctttatg
     1741 gattacatcg ccagccacga gcgggtgtgg gtgtgccgcc gggtggacat tgcccgccat
     1801 tggtgtgagg tgcatccgta tgcgccagcc gcgcgctgag cgccgcccga cacgggcgcc
     1861 tgattaaact gaattactga ataaaggaac acgccatgag tcatcctgtt tctgccgccg
     1921 tgcgggtggc cgccgccgtg gacttgcccg actgggcgcg tcgggcggtg aacctggccg
     1981 atgcccggct gggcgcgcgc gccttgggcg ccagcgacga attttttgcc ccgctggcgc
     2041 gcatgctcaa cccggagccg gcagtgtttg tgcccggcca gtacgacgcc cacggcaagt
     2101 ggatggacgg ctgggaaacc cgccgccgac gcgggccggg ccacgactgg agcatcatcg
     2161 gcctggcgcg gcgcggccgg gtgtttggct ttgacgtgga caccagccac ttcaccggca
     2221 attacgcgcc ggccatcgcc atcgaagcca ccgactgccc cagcgacgac cccgaggtgc
     2281 tggcacacgc cagctggacg ccgctgttga cctccaccag cctgcgcggc gacagccacc
     2341 atctgctggc ggtggtggac gacggggtgt ggagccatct gcgcgtgcac atctacccgg
     2401 acggcggcgt ggcccggctg cgcgtgtacg gccgcccggc ggaaatcacc gccggcccgg
     2461 acgggctgac cgacctggcc gcgcagatca acggcggccg cgcgctggcc tggaacgacg
     2521 cccactttgg ccaggcggcc aatctgctgg cgccggggcg tggggtgaat atgggcgacg
     2581 gctgggaaac ccgccgccga cgcgagccgg ggcacgactg gtgcattatc gagctgggcg
     2641 cgccgggggt gatcgagcgc atcgaggtgg acaccgccca cttcaagggc aattacccgg
     2701 accgtttttc catgcaggcg gcgcgggtgg acggcggcac cgagcaatcg gtgattaccc
     2761 agagcatgtt ctggccgacg ctgattggcg aaacgccgct gtcgatggac gccgagcatg
     2821 tgttcagcac gggcattgcc gcgctgggcg ccgtcagcca tgtgcgcttt aacatccacc
     2881 cggatggcgg cgtgtcgcgg ctgcggctgt gggggcggcc ggcatgatca ccctcaccgc
     2941 cagcgcgctg agcgccgacg cgtttgcccc gtttggccaa gtgctgtccg ccgccagcgc
     3001 ggcgcggcag ttcaccatca atggcggcaa caccgaacgt tttcatgatc tggccgagct
     3061 gtcggccggc gcggacggcc ggctgattgt cagcctgttt cgcggccagc cgcgcgcgct
     3121 gccgtttgcg gtgagcatgc tggagcgcca cccgctgggc agccaggctt tcttgccgct
     3181 gggcgaacgg ccgtatctgg tggtggtggc cccgcccggt gcgccgccca gcccggcaag
     3241 tgtgcgcgcc tttctcgccg cccccggcca gggggtgaac tatgcgccgg gagtgtggca
     3301 tcacccgctg ctggcgctgg acgcggtcag cgacttttgg gtgatcgacc gcgccggccc
     3361 cggcgacaac tgcgaagaaa tcaccctggc cgagccggtg tgggtgcagc ggccctgacg
     3421 taatcctccc tggcccggcc tgcgccgggc ggggactcag cgccgttcac cgcgtcattg
     3481 cacagccttg ccgtattgct tgcactgtct gcatccatct ctgcgcgcct ggccaggcgc
     3541 acgaccgcag acagtacaag gtgtacggca aggtcgggca agcacgccag gcgggttttg
     3601 ttcgccgcgc ttatccgcca tcaaggccag cccggccccc gctgcggcat catccgcaca
     3661 tgctttcttc tcattctccc gcgcccacgc cgcgcccggt gtggccggtc tggctctacc
     3721 tgagcgcgct gaccctggcg ctggccgccc tggccgagtg gcgcagccat gccgcgctgg
     3781 cggcgctggc gctgggcggc gcctggctca agggtgtggt catcatcgaa tctttcatgg
     3841 ccctgcgcga tgcgccgatg tggctgcgcg cggcggtgca tggctggctg gcgctgttgt
     3901 gcggcgggct gtgcgccagt tttctgtatg gaggctgaac atggccgcaa ctgatatccc
     3961 gttttatcaa ccggccggcg acgagtgcga ggtgttcgcc cacgcctggc gtcagcaact
     4021 gccggtgttg atcaaaggcc ccaccggctg cggcaaaacc cgctttgtcg cccatatggc
     4081 cgcgcagctg gggctgccgc tgtttaccgt gtcttgccat gatgatttgt ccgctgccga
     4141 tctggtgggc cgccacctga ttgccggcgg cgacacccgc tggtgcgatg gcccgctcac
     4201 ccaggcggtg cgcgctggcg ggctgtgtta tctggatgaa gtggtcgagg cgcgcaagga
     4261 caccacggtg gtgctgcacc cgctgaccga cgaccggcgc atgctgccca tcgagcgcac
     4321 tggcgaggtg ctgcaagcgc cgccgggctt tatgctggtg gtgtcgtaca accccggtta
     4381 tcagcatgtg ctgaaaaccc tgaaaccctc tacccgccag cgttttgtgg cgctgagttt
     4441 tgattttccg cgcccggaag tcgaatgcga ggtggtgcgc cacgaaggcg gcgtggacgc
     4501 cgagctggct tggcggctgg tgcggctggg cgggcaattg cgccagctgg gcggccacga
     4561 tctggacgaa ggcgccagca cccggctgct ggtgtacgcc gcccggctga tggccagcgg
     4621 catgccggcg cggcgcgcct gcgaggtggc gctggccgaa ccgctcagcg acgagccaga
     4681 caccctggcc gccttgcgcg cggtgatcga cacccaattc gggagctgag cgatggaaga
     4741 ctgggttggc ggctggtggg accggctgat ccgccgcgcc gcctatcgcg gccatccgca
     4801 ggcggcggtg gcgctggccg acatcgagcg ccaggcgccg gtgttttttc gcgccatggg
     4861 cggcgacccg gcgctgaccg tgcgcagcac ggtgggcgcg gcgcatggcg cgcggcggcg
     4921 gctgctggag cgggtggccg gcattggcga acgggtggag ctgggctggc gcgacgcgca
     4981 atcgctatac ctgcccgagc gcatcgacct ctacccggac gccgcgctga accgtgagct
     5041 gtatttctgg ctgatggcgc tggccgcgcg cgacgacgcc caccacggcg gcgactggtt
     5101 tgcccgcaac cgcgccgcca ccgccgcctg cctgcgccag atgcccggcc tggcggcggt
     5161 gtacgcccgg ctggccgccg ccctgctgcc gctgcgcgac ctgccgcgcc gcgccgacga
     5221 acgcgcgcag gaactggcca tccgcgccgc cctgcaacag ccgcatcagg ccatcgccct
     5281 gccgccctgc gcccagccgc cgcagccggt gccgctgtgg ctgcacccct cgccgcccgg
     5341 cggcggccag gcccattccg cccgccgcga tggcgacgcc accgccgagg acgaagccag
     5401 ccagcgccgc gacagcaagg cgcgccgccg ctatcgcgcc cgcgatggcg aaactgacga
     5461 gcgcaaaaac ggcatgctga tggtgtttcg cgccgaaagc ctgttcacct gggacgaata
     5521 cgtgcgggtg aaccgccaca ccgacgaaga cgacgacagc gacgacggcg ccgccgccga
     5581 agacatgcaa accctcaccg tgcagcgcga cacccgtcgc cgcgccagca ggctgaaatt
     5641 cgacctcgac ctgccggccg ccgcctacga cgacaccccg ctgggccccg gcatcctgct
     5701 gccggaatgg gactacaagc gccagcgcct ggtggccgac cacgcccgca tccagcccat
     5761 gctgccgcgc gacgccgccc cggcggcgct gcccgagcgg ctgcgcccgc tggcgcgccg
     5821 gctgcgccgc cagtttcagg cgctggcgcc agagcgcacg cgcaaacaag gcgaggccag
     5881 cggcccggac atcgacctgg accgcctggt gcgctttctg gccgaacgcg ccagcggcgc
     5941 ggcggtggcc gaaccgccgc tgtatcaagc ctggccgccc ggcgggcgca gcctggcctg
     6001 cttgctgctg gccgatattt cgctgtccac cgaaagctgg ctgggcgacg ccggccaggt
     6061 gattcaagtc atccgcgaca gcctgctgct gttcgccgag gcgctgaccg cctgcggcga
     6121 cgcctttggc ctgtatggtt tttcgtccat ccgccgcgag gaagtgcgct atctgctgct
     6181 gaaagacttt gccgaacgct acgacgacgc ctgtcgcggc cgcatcctcg ccctgcgccc
     6241 cggctactac acccgcctgg gcgccgccat ccgccactcc agccgcatcc tgctggaaca
     6301 agccgcccag cgccgcgtgc tgctgatcct ctccgacggc aaacccaacg acctggacca
     6361 ctacgaaggc cgctacggcg tggaagacac ccgccaggcg gtgctggaag cccgccgcgc
     6421 cgggctgatt ccgttttgcg tcaccatcga ccgccaggcc ggcgactatc tgccccacct
     6481 gtttggccgc cacggctaca ccgtcgccca cgacgcccgc cgcctgccgc aagtgctgac
     6541 gaggttgtat gcggggttga cggggtgaga ag
//
LOCUS       DAOPYX010000166         6538 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_14414_length_6538_cov_9.154712, whole genome shotgun sequence.
ACCESSION   DAOPYX010000166 DAOPYX010000000
VERSION     DAOPYX010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 6538)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6538)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6538
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14414_length_6538_cov_9.154712"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..537
                     /locus_tag="PKN55_09175"
     CDS             <1..537
                     /locus_tag="PKN55_09175"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNX71458.1"
                     /translation="LGYLPQSWRDAFACQVLEALLIARHPHRQGYWDSAADIASARAA
                     LAALDIGHLAARDIRTLSGGERQRVAVAALLTQDAPLLLLDEPAAALDIGHQAQLLAL
                     LAARCQRPGHAVMLVSHELNLTLQHASHALLLLDDGRWRALPVAELSAELLSACLGHP
                     LLEIQRDGRRYWLPAAGP"
     gene            616..1245
                     /gene="cobO"
                     /locus_tag="PKN55_09180"
     CDS             616..1245
                     /gene="cobO"
                     /locus_tag="PKN55_09180"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018152598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HNX71459.1"
                     /translation="MTAAATPSEDPARAERHRARMARKKALIDERIAQAQGQAGVLLV
                     TCGNGKGKSSSAFGMVARALGHGMKVGIVQFIKGAIATGEEQFFRRFPDEVQFHVMGE
                     GYTWNTQDRARDIASAERAWDKACELLRDPAIGLVVLDELNIALKYQYLDLEKVLDDL
                     AARPPMQHVVVTGRGAPPALIELADTATDMQLLKHAFASGIAAQPGIEW"
     gene            1242..2528
                     /locus_tag="PKN55_09185"
     CDS             1242..2528
                     /locus_tag="PKN55_09185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011135108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042242 - cobyrinic acid a,c-diamide
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyrinate a,c-diamide synthase"
                     /protein_id="HNX71460.1"
                     /translation="MSARAILVAAAASGQGKTTVTAALARRLRRDGARVRVFKTGPDF
                     LDPLTLARASGAEVDTLDLWMVGEAGCRQRLAAAAQDADWILVEAVMGLYDGAPSSAD
                     LANRFGLPVLAVLDARAMAQTAGALALGLRDYGPVQLAGVLANRVAGDSHAAMVAESL
                     RDIPLLGHLPRQAQSLPERHLGLGLPDELADFDARLDALADSLVLDDAAWQALPRLTL
                     PPEPAPALPPLLAGRRLALARDAAFAFVYPANLACLRALGAELVEFSPLADQPLPAGV
                     DALYLPGGYPELHAETLNRARTWQASVRAAHAAGLPIWAECGGMMALADAIETVDGHS
                     WGMAGLLPGVAHMQDRLAGLGGQAWGALRGHTFHYSRLELPPEPQAHTVSQRGGGRGE
                     AIYRVGSLQASYFHAYFPSAPEQAAALFLPAEDRPC"
     gene            2522..3481
                     /gene="cbiB"
                     /locus_tag="PKN55_09190"
     CDS             2522..3481
                     /gene="cbiB"
                     /locus_tag="PKN55_09190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019924914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016880 - acid-ammonia (or amide) ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-phosphate synthase CbiB"
                     /protein_id="HNX71461.1"
                     /translation="MLSGLPAWLLALCLLAGVALDAWWGEPRRWHPLVGFGWLAQRLE
                     ARLNQGGARLWRGALAWALLVAPAPLALAALLAALPDSPSGWALAAGLHALALYAALG
                     LRSLAEHTRPIARALAAGRLDEARQRTGWIVSRDTAHASPDELAKATVESLLENGNDA
                     VFGALFCFMLAGGPGVLAFRLANTLDAMWGYRSPRFNLFGRCAARLDDVFNYLPARLT
                     ALSYALLGERQRAWRSWRSQAPAWPSPNAGPVMAAGAGALGLSLGGAARYDGIDEIRP
                     PLGEGRPAAPADIERAWRLVRGSCLLWLALAVLAAASWEWACR"
     gene            3472..4506
                     /gene="cobD"
                     /locus_tag="PKN55_09195"
     CDS             3472..4506
                     /gene="cobD"
                     /locus_tag="PKN55_09195"
                     /EC_number="4.1.1.81"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019924915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0048472 - threonine-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine-phosphate decarboxylase CobD"
                     /protein_id="HNX71462.1"
                     /translation="MPLTALLTPPPRPPHGGNLHAAIARFGGAPADWLDLSAALNPQP
                     YPPPPLPDGLWQALPPPPSALLDAARSYYRADALLASAGSQAAIHTLPRLRRASQVTL
                     ATPMYAEHRWGWQAAGHRLCDCPHQAEALLHAAGHSEVLVLCQPNNPGGECVEPEVLL
                     ACARRLAERGGWLLVDEAFIDATPEASVISQVHAHPNLIVLRSLGKFFGLAGLRLGMV
                     AAHPAILAALDAALGPWTISAAAQHIARAALTDANWIAAARQRLPQDSARLALLLAEH
                     GLASVGTALFRTCPHPHAEALHQALAAQRVWTRVLPGALRFGLPGGERQWQQLAQSLA
                     ADSCRHVIRR"
     gene            complement(4563..6242)
                     /locus_tag="PKN55_09200"
     CDS             complement(4563..6242)
                     /locus_tag="PKN55_09200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011135274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HNX71463.1"
                     /translation="MSAAASPTARWFRPRLLDTLHGYSFATFRDDLAAGVVVGIVALP
                     LAMAFAMASGVSPQAGIFTAVIAGFLISALGGTRLCIGGPTGAYIVILYGIAVKYGVA
                     NLLLCTFLAGLMLIAMGLLRMGQIIKFFPMPLVTGFTNGIAVLIFLTQLKDFFGLPVA
                     QMPSGFFATLQTLNQTLAQFHPATLALSAGCLALILFWPKKLNKLLPAPFVALVLASV
                     LTAALALPVATIGSKFGGIPQGLPEFRMLTLDLNHLSDLLAPAFTIALLGAIESLLCA
                     VVADSMTGDKHDANQELIAQGIANVVVPFFGGIAATGAIARTATNINNGGKTPMAGII
                     HALLLLLIILVAAPLARYIPLAALAAILISVALKMGDWDIRKALHFPKRDTLIVAVTF
                     ALTVAFDLTVAVQIGLLLAAVFFIQRMASSTTVQKLQPEHAYLFERHSIAGKTLPDGC
                     AAFRVEGALFFGAADTLDTVLAGSAGCKVVILQMHRLVLLDTTGLLALDELNDKLRQG
                     GRALVLCGAGGDTAKMLVDSQLAEHVGERNVQPDLAAALTRAQELLRTDSV"
BASE COUNT         1014 a         2298 c         2235 g          991 t
ORIGIN      
        1 ctgggctatt tgccgcaaag ctggcgcgac gcctttgcct gccaggtgct ggaggcactg
       61 ctgattgccc gccacccgca tcgccagggg tattgggaca gcgccgccga catcgccagc
      121 gcgcgcgccg cgctggcggc gctggacatc ggccacctgg ccgcgcgcga catccgcacc
      181 ctgtccggcg gcgagcgtca gcgggtggcg gtggccgcgc tgctgaccca ggatgcgccg
      241 ctcttgctgc tggacgaacc cgccgccgcg ctggacatcg gtcaccaggc gcaattgctg
      301 gcgctgctgg ccgcgcgctg ccaacgcccc ggccacgcgg tgatgctggt cagccatgag
      361 ctgaacctca ccctgcaaca cgccagccac gcgctgctgt tgctggacga cggccgctgg
      421 cgggccttgc cggtggccga actcagcgcc gagctgctca gcgcctgcct gggccatccg
      481 ctgctggaga tccagcgcga tggccgccgt tactggctgc cggccgccgg gccgtagcat
      541 acggccaaat aacgcatcaa aattgccacg tttttcaggc atcatcgccc tgctttttcg
      601 ccaaggacgc cccccatgac cgctgctgca accccttcgg aagaccccgc ccgcgccgaa
      661 cgccaccgcg cccgcatggc gcgcaaaaag gcgctgattg atgagcgcat cgcccaggcg
      721 caaggccagg ccggcgtgct gctggtgaca tgcggcaatg gcaagggcaa aagctccagc
      781 gcctttggca tggtggcgcg cgcgctgggc catggcatga aagtgggcat tgtccagttc
      841 atcaagggcg ccatcgccac tggcgaagag cagttttttc gccgttttcc cgacgaagtg
      901 cagtttcatg tgatgggcga gggctacacc tggaacaccc aggaccgcgc ccgcgacatc
      961 gccagcgccg aacgcgcctg ggacaaggca tgcgagctgc tgcgcgaccc ggccattggc
     1021 ctggtggtgc tggacgagct gaacatcgcc ctcaaatacc aatacctcga cctggaaaaa
     1081 gtcctcgatg acctggccgc ccgtccgccg atgcagcatg tggtggtcac cggccgtggc
     1141 gcgccgcccg cgctgatcga gctggccgac accgccaccg acatgcagct gctcaagcac
     1201 gcctttgcca gcggcattgc cgcccagccg gggatcgagt ggtgagcgcg cgcgccatcc
     1261 tggtggccgc cgccgcctcg ggccagggca aaaccacggt caccgccgcg ctggcgcgcc
     1321 ggctgcgccg tgacggcgcg cgggtgcggg tgttcaaaac cgggccggat tttcttgatc
     1381 cgctgaccct ggcgcgcgcc agcggcgccg aggtggacac cctggattta tggatggtgg
     1441 gcgaagccgg ctgccgccag cgcctggccg ccgccgcgca agacgccgac tggattctgg
     1501 tggaagccgt gatgggcttg tacgacggcg cgccctcgtc cgccgacctg gccaatcgct
     1561 ttggcctgcc ggtgctggcg gtgctggacg cgcgcgccat ggcgcaaacc gctggcgcgc
     1621 tggcgctggg cctgcgcgac tacggcccgg tgcagctggc cggcgtgctg gccaaccggg
     1681 tggccggcga cagccacgcc gccatggtgg ccgaatcgct gcgcgatatt ccgctattgg
     1741 gccatttgcc gcgccaggcg cagtcgctgc ccgagcgcca tctgggcctg ggcctgcccg
     1801 acgagctggc cgactttgac gcccggctgg acgcgctggc cgacagcctg gtgctggacg
     1861 acgccgcctg gcaggcgctg ccccgcctga ccctgccgcc cgagccggcc ccggcgctgc
     1921 cgccgctgct ggccggccgc cggctggcgc tggcgcgcga cgccgcgttt gcctttgtct
     1981 acccggccaa tctggcctgc ctgcgcgcgc tgggcgctga gctggtggaa ttctcgccat
     2041 tggccgacca gcccctcccg gccggggtgg acgcgctgta tctgcccggc ggctaccccg
     2101 agctgcacgc cgaaacactc aaccgcgccc gcacctggca agccagcgtg cgcgccgccc
     2161 acgccgccgg cctgccgatc tgggccgagt gcggcggcat gatggcgctg gccgacgcca
     2221 tcgaaaccgt ggacggccac agctggggca tggccggcct gctgccgggc gtggcgcata
     2281 tgcaagaccg gctggccggc ctgggcgggc aagcctgggg cgcgctgcgc gggcatacgt
     2341 ttcattattc gcggctggag ctgccgcccg agccgcaggc gcacaccgtc agccagcgcg
     2401 gcggcgggcg cggcgaggcg atttatcggg tgggcagttt gcaggccagt tattttcatg
     2461 cctactttcc gtccgccccc gagcaggcgg cggccttgtt cttgcctgcg gaagaccgcc
     2521 catgctgagc ggcttgccgg cctggttgct ggcgctgtgc ctgctggccg gcgtggcgct
     2581 ggacgcctgg tggggcgagc cgcgccgctg gcatccgctg gtgggctttg gctggctggc
     2641 gcagcggctg gaagcgcggc tcaaccaagg cggcgcgcgg ttgtggcgcg gcgcgctggc
     2701 ctgggcgctg ctggtggccc cggcgccgct ggcgctggcg gcgctcttgg ccgccttgcc
     2761 cgacagcccc agcggctggg cgctggccgc cggcctgcac gcgctggcgc tgtacgccgc
     2821 gctgggcctg cgcagcctgg ccgaacacac ccgaccgatt gcccgcgcgc tggccgccgg
     2881 ccggctggac gaagcgcgcc agcgcaccgg ctggattgtc agccgcgaca ccgcccacgc
     2941 cagcccggac gaactggcca aggccaccgt tgaatccctg ctggaaaacg gcaacgacgc
     3001 ggtgtttggc gcgctgttct gctttatgct ggccggcggc cccggcgtgc tggccttccg
     3061 gctggccaac accctggacg ccatgtgggg ctaccgctcg ccgcgcttta atttatttgg
     3121 ccgctgcgcc gcccggctgg acgatgtgtt caactacctg ccggcgcggc tgactgcgct
     3181 cagctatgcg ctgctgggcg agcgccaacg cgcctggcgc agctggcgca gccaagcgcc
     3241 ggcctggccc tcgcccaacg ccggcccggt gatggccgct ggcgctggcg cgctgggcct
     3301 cagcctgggc ggcgcggcgc gctacgacgg catcgacgaa atccgcccgc cgctgggcga
     3361 aggccggccc gccgcgcccg ccgacatcga acgcgcctgg cggctggtgc gcggcagctg
     3421 cctgttatgg ctggcgctgg ccgtgctggc cgccgccagc tgggagtggg catgccgctg
     3481 accgccctgc tcaccccgcc gccgcgcccg ccgcacggcg gcaatctgca cgccgccatc
     3541 gcccgctttg gcggcgcgcc ggccgattgg ctggatttgt ccgctgcgct gaacccgcag
     3601 ccctacccac cgccgccgct gccggatggc ctgtggcagg cgctgccacc gccgcccagc
     3661 gcactgctgg acgccgcgcg cagctactac cgcgccgatg cgctcttggc cagcgccggc
     3721 agccaggccg ccatccacac cctgccgcgc ctgcgccgcg ccagccaggt gacgctggcg
     3781 acgccgatgt acgccgaaca ccgctggggc tggcaagccg ctggccacag gctatgcgac
     3841 tgcccgcacc aggccgaggc gctgctgcac gccgccggcc acagcgaggt gctggtgctg
     3901 tgccagccca ataaccccgg cggcgaatgc gtcgagccgg aggtgctgct ggcttgcgcg
     3961 cggcggctgg ccgagcgcgg cggctggctg ctggtggacg aagcctttat tgacgccacc
     4021 cccgaggcca gcgtgatcag ccaggtgcac gcccacccca atctgattgt gctgcgctcg
     4081 ctgggcaaat tctttggcct ggccgggctg cgcctgggca tggtggccgc ccacccggcc
     4141 atcctggccg cgctggacgc cgcgctaggg ccatggacga tcagcgccgc cgcccaacac
     4201 atcgcccgcg ccgcgctgac cgacgccaac tggatcgccg ccgcccgcca acgcctgccg
     4261 caagacagcg cccgtctggc gctgctgctg gctgagcacg gcctggcctc ggtgggcacg
     4321 gcgctgtttc gcacctgccc acacccgcac gccgaggccc tgcaccaggc gctggcggcg
     4381 caacgggtat ggacacgcgt attgcccggc gcgctgcgct ttgggttgcc gggcggggaa
     4441 aggcagtggc agcaactggc gcaaagtctg gcggccgatt catgccgaca tgtcatacgc
     4501 cgataaaaac atcaagccgg ctatccatct gccggcttga tgttttcagg cgtaggcaaa
     4561 cgtcacacac tatccgtacg cagcaactcc tgtgcgcggg tcagcgcggc ggccaggtct
     4621 ggctgcacat tgcgctcgcc aacatgctcg gccagttgcg aatccaccag catcttggcg
     4681 gtgtcgccgc cggcgccgca cagcaccagt gcgcggccgc cctggcgcag cttgtcgttg
     4741 agctcgtcca gcgccaataa gccggtggtg tccagcagca ccagccggtg catttgcaaa
     4801 atcaccacct tgcagccggc gctgccggcc agcacggtgt ccagggtgtc ggcggcgcca
     4861 aaaaacagcg cgccttccac ccgaaacgcc gcgcagccgt cgggcagggt tttgccggca
     4921 atcgagtggc gctcaaacag atacgcgtgc tcgggttgca gcttttgcac cgtggtgctg
     4981 ctggccatgc gctggataaa aaacaccgcc gccagcaaca ggccgatttg caccgccacg
     5041 gtaaggtcaa acgccacggt cagggcaaaa gtcaccgcca caatcagcgt gtcgcgcttg
     5101 ggaaaatgca gcgccttgcg gatgtcccag tcgcccattt tcagcgccac cgaaatcaag
     5161 atcgccgcca gcgccgccag ggggatatag cgcgccagcg gcgcggcaac caggatgatc
     5221 agcagcagca agagggcgtg gatgatgccg gccattggcg ttttgccgcc gttattgata
     5281 ttggtggcgg tgcgggcaat ggcgccggtg gcggcgatgc caccaaagaa cggcaccact
     5341 acattggcga tgccttgggc aatcagctct tgattggcat cgtgcttgtc gccggtcatt
     5401 gaatctgcca ccaccgcgca cagcaaggat tcaatcgccc ccagcagggc aatggtgaag
     5461 gctggggcca aaaggtcgga caggtggttc agatccagcg tcagcatgcg aaattccggc
     5521 aggccctgcg ggatgccgcc aaacttgctg ccgatggtag ccaccggcag cgccagcgcg
     5581 gcagtcagca cactggccag caccagcgcc acaaacggcg cgggcagcag tttgttgagc
     5641 tttttcggcc agaacaaaat cagcgccagg cagccggccg acagcgccag ggtggccggg
     5701 tgaaactgcg ccagcgtttg gttcagcgtt tgcagggtgg caaaaaaccc tgagggcatc
     5761 tgcgccaccg gcaggccaaa aaagtctttc agctgagtca gaaagatcag cacggcaatg
     5821 ccattggtga agcccgtcac cagcggcatg gggaaaaact tgatgatctg tcccatgcgc
     5881 aacaggccca tggcgatcag catcaagccg gccaaaaacg tgcacagcaa taaattggcc
     5941 acgccgtact tcacggcgat gccgtacagg atgacaatat acgccccggt gggcccgcca
     6001 atgcacaggc gggtgccgcc cagcgcggaa atcaaaaagc cggcaatcac cgcggtgaaa
     6061 atgccggctt gcgggctgac gccgctggcc atggcaaacg ccatcgccaa gggcagggcg
     6121 acgatgccaa ccaccacgcc ggcggccaga tcgtcacgaa acgtagcaaa tgaataccca
     6181 tgcagggtgt ccagcaggcg cgggcgaaac cagcgtgctg tgggtgaggc ggcagcagac
     6241 atgaattggg gcttccttgt gccggcaggc gccgggatag tctctctcct gatccggccg
     6301 gcgctcttgg ccacggcgca ccctgaacat ggcgccgggc ggcttctgca tggccgcgtc
     6361 tggcgcaggg tgtggctggc gtgctggcgt cttgccgccc tggggcgggg gccgggacga
     6421 tgcggaaggc tcgcatggcc ttcttggcgc ggctcacaaa actcagcgaa gcgatcctgg
     6481 ctaggcgtgc gcagacagtg cgggtagtac ggcaaggtcg ggcaacaacg ccaggagg
//
LOCUS       DAOPYX010000167         6516 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_14479_length_6516_cov_11.371614, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000167 DAOPYX010000000
VERSION     DAOPYX010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 6516)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6516)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6516
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14479_length_6516_cov_11.371614"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            301..2304
                     /locus_tag="PKN55_09205"
     CDS             301..2304
                     /locus_tag="PKN55_09205"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNX71464.1"
                     /translation="MLHSLSLRAKILLPFIILMLAGFAVVVGYNSWAMRQHDLEQGVE
                     NARLQAAVLSTSINKTLNDGLSTTQTLTSTFETLRRSHVVNRDMLNKILARQLENHAG
                     LRAVWTGWEPDALDGHDSEYAGQKPAHDDSGRFVPYWHRDTQGISVEPLVDYDKPGAG
                     DYYLLPKQTGSLQVIEPYLYPVNGKPVMTTSIVAPLMVGITFSGVVGVDIALTDIANE
                     MAKVKPYETGYLSLLSGSGQWLAHPQEDKVGKPAKDELPAEALEAIKAGKPWQLEDAN
                     GMLHFFTPIKVHEGMTPWSLRVSVERAQLLANADRARNAALLLGAGACLATLLLCAVL
                     LGWLTRPLVGLAEAVEVLAQGEGDLTRRLPVNSQDEIGRTSAAFNRFMDKLRDMFAEV
                     KQHAATLETSLVRLGDTTQAVAGNARQQADAASSTAATVEEVSSSISQIADGAQQAAR
                     AAEDTGELSEQLAELVQDTAGEIRQAASAVEALSGTLNGLGERSQQIASIVDVIRDIA
                     DQTNLLALNAAIEAARAGEQGRGFAVVADEVRKLAERTASATLEIATMIQTIQRDMQA
                     ATQGMDTALGRISSGVAHSEDTAEAIAGIHDTMANMVNRIRDIARATEEQSVASQDIS
                     SHVEHINHMSQQTDQQIHVASEETAQLRALGQQLEALVQRFRT"
     assembly_gap    2448..2547
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2566..5340)
                     /gene="polA"
                     /locus_tag="PKN55_09210"
     CDS             complement(2566..5340)
                     /gene="polA"
                     /locus_tag="PKN55_09210"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011134334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HNX71465.1"
                     /translation="MKTLLLVDGSSYLYRAFHAMPDLRSPAGEPTGAVYGMINMLKKL
                     EKDISFDYSAVVFDAKGKTFRDELYPEYKAQRPPMPPELAAQITPVHAAVTGLGWPLL
                     MVEGVEADDVIGALAHQASTQGWQVVVSTGDKDMAQLVNAQVTLVNTMTSETLDSAGV
                     QEKFGVPPERIIDFLTLTGDKVDNVPGVDGCGPKTAAKWLAEHGSLDAIIAAADSVKG
                     KAGEKLRAALPHLPLSRQLVTIKLDVDLSAHLADGLASLRHGEPRRDALLALYRQCGF
                     RTWLRELDAAASEPAPPGDLFAEAPAAPTADAAPAASAAPKPAPASANYTLIRTQAEL
                     DAWLARLQAAPVVSLDTETTSLDSLRARLVGLSFSLTPGEAAYLPLMHRDAGGLLPDQ
                     LSLDAALATLKPWLENPQAKKLGQNLKYDRHVLANHGVQLAGVVDDTLLASYVLESHL
                     THNMDDLAERHLGVSTVSYEALCGKGAKQIGFDEVDIDAAGHYAAEDADITLRLAHAF
                     APQLHGALEDVYRRIELPTAEVLFAMERTGVLIDRDELARQSHQLGQRIQQLEARAYE
                     LAGQPFNMNSPKQLQEILFGRLGIDSKGLKKTPTGAISVNEDALEKLAADHPLPKLLL
                     EYRGLAKLKSTYTDKLPTMIHPDTGRVHTHYSQAVAVTGRLASSDPNLQNIPVRTEEG
                     RRVRKAFIAPPGWQIVSADYSQIELRIMAHLSDDPRLLQAFADGEDIHRATAAEVFAV
                     PLAEVSSQQRRYAKTINFGLIYGMSAFGLAAQLEIDRSAAQSFIDRYFARYPGVADYM
                     ARTREHAREQGYVETVFGRRLWLPDIHAANAMKRQGAERAAINAPMQGTAADLIKLAM
                     IEVQHWLRTENLRSRLIMQVHDELVLEAPADELDTVKAGVVAAMARVAQLKTPLVAEV
                     GSGDNWEAAH"
     gene            5355..6083
                     /locus_tag="PKN55_09215"
     CDS             5355..6083
                     /locus_tag="PKN55_09215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011134333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HNX71466.1"
                     /translation="MSVSDKVPQASDRLEHMQRFRSREAWHVLKIMAEFVESTEALSE
                     ISPAVSIFGSARTPRAHGYYKLTEEIARRLSDAGFSVISGGGPGIMEAASKGAFFGKS
                     PSVGLNIQLPHEQKPNEYQDLSLSFNHFFSRKVMFVKHAVAYVVMPGGFGTLDEMFEA
                     LTLVQTGKSRKIPIILVGRAFWQGLLDWFQAQLVAEGMIAADDMQLIQLIDEPGEIVE
                     AIFRHYEMRGFEALPEEREQLLNL"
     gene            6110..6424
                     /locus_tag="PKN55_09220"
     CDS             6110..6424
                     /locus_tag="PKN55_09220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008952206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2782 domain-containing protein"
                     /protein_id="HNX71467.1"
                     /translation="MRRILIALSLLSPLAALAAPANVPPPPTIQESAAAQAEPEVRII
                     QKSGQKIEEYRMNGKLYAIKVTPKIGLPYYLVDEDGSGKMNRIDTHGDKLVVPRWVLM
                     TF"
BASE COUNT         1136 a         1970 c         2123 g         1187 t
ORIGIN      
        1 ggcacggggc cgaatgaggg cggcccatcc atgtgtcgtg caccacaagg cggcaagcct
       61 tgccaagcgt cgctaagagc ggctaacaaa accctcctgg cgttgttgcc cgaccttgcc
      121 gtacgactta tactgtctgc ggtcgcgcgc ctggccagga tcgctttgct gagttttgtg
      181 agtcgcgcta aatgaccatg cgccacgtag cgcatggcgc acacctacgc acacaaccgg
      241 agaccgcgcc cctggcgcgg cagaggccgc cgtatggctt cgaacaaggg gagattcaat
      301 atgctgcatt cgctcagcct gcgcgccaaa attctattgc cgttcatcat cctgatgctc
      361 gccggctttg ccgtggtggt gggctacaac agctgggcca tgcgccagca cgacctggag
      421 caaggcgtag aaaacgcccg gctacaggcc gccgtgctgt ccaccagcat caataaaacg
      481 ctgaacgatg ggctgagcac cacgcagacg ctgacttcca ccttcgaaac cctgcgtcgc
      541 agccatgtgg tcaaccgcga catgctcaac aagattctcg cccgccagct ggagaaccac
      601 gccggcctgc gggcagtgtg gaccggctgg gagccagacg cgctggatgg ccacgacagc
      661 gaatacgccg ggcaaaagcc cgcccatgac gacagtggcc gctttgtgcc ctactggcac
      721 cgtgataccc agggcatcag cgttgagccg ctggtggatt acgacaagcc cggcgccggc
      781 gattactacc tgctgcccaa gcaaaccggc agcttgcagg tgattgaacc ttatctgtat
      841 ccggtgaatg gcaagccggt gatgacgacc tccatcgtcg cgccgctgat ggtgggcatt
      901 actttttctg gcgtggtggg cgtggacatc gcgctgaccg acatcgccaa cgaaatggcc
      961 aaggtcaaac cctacgaaac cggctacctc agcctgctgt cgggcagcgg gcaatggctg
     1021 gcgcatccgc aagaagacaa agtcggcaag ccggccaagg atgagctgcc ggccgaagcg
     1081 ctggaggcca tcaaggcggg caagccctgg caactggaag acgccaacgg catgctgcat
     1141 ttctttaccc cgatcaaggt gcacgagggc atgacgccgt ggtccttgcg cgtcagcgtc
     1201 gagcgcgccc aactgctggc caacgccgac cgcgcgcgca acgccgcact gctgctgggc
     1261 gctggcgcct gcctggccac cttgctgctg tgcgccgtcc tgctgggctg gctgacccgc
     1321 ccgctggtgg gcctggccga agcggtggaa gtgctggcgc aaggcgaggg cgacctcacc
     1381 cgtcgtctgc cggtgaacag ccaggatgaa atcggccgca cctcagcagc gttcaaccgc
     1441 tttatggaca agctgcgcga tatgttcgcc gaggtcaagc aacacgccgc cacgctggaa
     1501 accagcctgg tgcgcttggg cgacaccacc caggcggtgg ccggcaacgc ccgccagcaa
     1561 gccgacgccg ccagctcgac cgccgccacg gtggaagaag tcagcagcag catcagccag
     1621 attgccgacg gcgcccagca agcggcgcgc gccgccgaag acaccggcga attgtccgaa
     1681 caactggccg aactggtgca ggacaccgcc ggcgaaatcc gccaggccgc cagcgcggtg
     1741 gaggcgctgt ccggcacgct gaacggcctg ggcgagcgtt cgcagcaaat cgccagcatt
     1801 gtcgatgtga tccgcgacat tgccgaccaa accaatctgc tggcgctgaa tgcggcgatt
     1861 gaagctgccc gcgccggaga acaaggccgt ggctttgccg tggtggccga tgaggtgcgc
     1921 aagctggccg agcgcaccgc cagcgccacg ctggaaatcg ccaccatgat ccagaccatc
     1981 cagcgcgata tgcaggccgc cacccaaggc atggacaccg cgctgggccg catcagcagc
     2041 ggcgtggcgc attcggaaga cactgccgag gcgattgccg gcattcatga caccatggcc
     2101 aatatggtca accgcatccg cgacatcgcc cgcgccaccg aagaacaatc ggttgccagt
     2161 caggacatca gcagccatgt cgagcacatc aaccacatga gtcagcagac tgatcagcag
     2221 attcatgttg ccagcgaaga aaccgcgcaa ctgcgggcat tggggcaaca gctggaagcg
     2281 ctggtgcagc gttttcgcac ctgagcggtg gtttccgtgg ccgcacacca cgcactgggc
     2341 tgagtggtgt gcggcagaag ctgtcgccgc ccgaaacggc ctggggcatc gtcagttttg
     2401 cctggtgctg tgtctgggcg cggccgccca atgtctcagt gcgcggcnnn nnnnnnnnnn
     2461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2521 nnnnnnnnnn nnnnnnnnnn nnnnnnngcg cggccgccca atgtctcagt gcgcggcttc
     2581 ccagttatcc ccgctgccga cttccgccac cagcggggtt ttcagttgcg cgacgcgcgc
     2641 catggcggcc actacgccgg ctttgacggt gtccagttcg tcggccgggg cttccagcac
     2701 cagttcgtcg tgtacttgca tgatcagccg gctgcgcagg ttttcggtgc gcagccagtg
     2761 ttgcacttca atcatcgcca gtttgatcag gtcggcggcg gtgccttgca tcggggcgtt
     2821 gatggcggcg cgttcggcgc cttggcgttt catggcgttg gcggcgtgga tgtcgggcag
     2881 ccacaggcgg cggccaaaga cggtttccac atagccttgt tcgcgcgcgt gctcgcgggt
     2941 gcgcgccatg tagtcggcca cgccggggta gcgggcaaag tagcggtcga taaagctttg
     3001 cgcggcgctg cggtcgattt ccagttgggc ggccaggcca aaggcgctca tgccgtagat
     3061 caggccaaag ttgatggttt tggcgtagcg gcgctgctgg ctgctgactt cggccagcgg
     3121 cacggcaaac acttcggcgg cggtggcgcg gtggatgtct tcgccgtcgg caaaggcttg
     3181 cagcaggcgc gggtcgtcag acagatgcgc catgatgcgc agttcgatct gtgagtaatc
     3241 cgccgacaca atctgccagc cgggcggggc gataaacgcc ttgcgcacgc ggcggccttc
     3301 ttcggtgcgc accgggatgt tttgcagatt ggggtcgctg ctggccaggc ggccggtgac
     3361 ggccacggct tgcgagtaat gggtgtgcac gcggccggtg tccgggtgga tcatggtcgg
     3421 cagcttgtcg gtgtaggtgg atttcagctt ggccaggccg cgatattcca gcagcagctt
     3481 gggcagcggg tggtcggcgg cgagtttttc cagggcgtct tcgttgaccg aaatcgcccc
     3541 ggtcggggtt tttttcaggc ccttgctgtc gatgcccagc cggccgaaca ggatttcctg
     3601 caattgcttg ggcgagttca tgttaaacgg ctggccggcc agttcgtagg cgcgggcttc
     3661 cagttgctgg atgcgctggc ccagttggtg gctttgccgg gccagttcgt cgcggtcgat
     3721 cagcacgcca gtgcgctcca tggcgaacag cacctcggcg gtgggcagtt cgatgcggcg
     3781 atagacgtct tccagcgcgc cgtgcagttg cggggcaaag gcgtgcgcca ggcgcagggt
     3841 gatgtcggcg tcttcggcgg cgtaatggcc agcggcgtcg atgtccactt cgtcaaagcc
     3901 gatttgtttg gcgcctttgc cgcagagcgc ttcgtagctg acggtgctta cgcccagatg
     3961 gcgctcggcc aggtcgtcca tattgtgggt gaggtggctt tccagcacat acgaggccag
     4021 cagggtgtcg tccaccacgc cggccagttg cacgccgtgg ttggccagca cgtggcggtc
     4081 gtatttgagg ttctggccga gttttttggc ttgcgggttt tccagccagg gtttcagcgt
     4141 ggccagcgcg gcgtccaggc tgagctgatc gggcagcagg ccgccggcat cgcggtgcat
     4201 cagcggcagg taggcggctt cgccgggggt gaggctgaag ctcagaccca ccagtcgggc
     4261 gcgcagcgaa tccaggctgg tggtttccgt gtccagcgac accacaggcg cggcttgcag
     4321 gcgggccagc caggcgtcca gctcggcctg ggtgcggatc agcgtgtagt tggcggatgc
     4381 cggcgccggc ttgggcgcgg cgctggcggc tggggcggca tcggcagttg gcgcggcggg
     4441 cgcctcggca aataaatcac cgggcggggc cggctcgctg gcggcggcgt ccagctcgcg
     4501 cagccaggtg cgaaacccgc actggcggta cagcgccagc agcgcgtcgc ggcgcggttc
     4561 gccgtggcgc aggctggcca ggccgtcggc caggtgggcg gacaggtcta cgtccagctt
     4621 gatggtgacc agttgccgcg acagcggcag gtgcggcagc gcggcgcgca gtttttcgcc
     4681 ggccttgcct ttgacgctgt cggcggcggc gatgatggcg tccaggctgc cgtgttcggc
     4741 cagccatttg gcggcggttt tcgggccgca gccgtccacg ccgggcacgt tgtccacttt
     4801 gtcgccggtc agcgtcagaa aatcaatgat gcgctcgggc ggcacgccga atttttcttg
     4861 caccccggcg ctgtccaggg tttcgctggt catggtgttg accagggtga cctgggcgtt
     4921 gaccagctgg gccatgtcct tgtcgccagt ggacaccacc acctgccagc cttgcgtgct
     4981 ggcctggtgc gccagcgcgc caatcacgtc gtcggcctcg acgccttcca ccatcagcag
     5041 cggccagccc aggccggtga cggcggcgtg cactggcgtg atctgcgcgg ccagctcggg
     5101 cggcattggc ggacgctggg ctttgtactc gggataaagc tcgtcgcgga aggtcttgcc
     5161 ttttgcgtca aatacgacag cgctataatc aaagcttatg tccttctcca gctttttcag
     5221 catgttgatc atgccgtaca ccgcgccggt gggttcgccg gccggcgaac gcaagtccgg
     5281 catggcgtgg aatgcgcggt aaagataaga agaaccgtcg accagtaaca gggttttcat
     5341 gaaagcaatc cgcaatgagc gtgtctgata aagtgccgca ggccagcgac cgtctggagc
     5401 acatgcagcg ttttcgctcg cgcgaagcct ggcatgtgct gaaaatcatg gctgaatttg
     5461 ttgagtccac cgaggcgctc agtgaaatca gtccggcggt gagcattttt ggcagcgcgc
     5521 gcacgccgcg cgcgcatggc tattataagc tgaccgaaga aatcgcccgc aggctttccg
     5581 acgccggctt ttcggtgatt tccggcggcg ggccgggtat catggaagcc gccagcaagg
     5641 gcgcgttttt tggcaaaagc ccgtcagtgg gcttgaacat ccagctgccg cacgagcaaa
     5701 agcccaacga gtatcaagac ctcagcctga gcttcaatca ctttttttcc cgcaaggtga
     5761 tgttcgtcaa gcacgcggtg gcctatgtgg tgatgcccgg cggctttggc acgctggacg
     5821 agatgttcga ggcgctgacc ctggtgcaaa ccggcaaaag ccgcaagatt cccattattt
     5881 tggtggggcg cgcgttctgg caggggctgc tggattggtt ccaggcccag ctggtggccg
     5941 agggcatgat tgccgccgac gatatgcagc tgatccagct gattgacgag ccgggcgaga
     6001 ttgtcgaggc gattttccgc cattacgaaa tgcgcggctt cgaggccttg cccgaagaac
     6061 gcgaacaact gcttaatctt taatcatccc tgcttgcacg aggcccacca tgcgccgcat
     6121 tcttattgct ctttccctgc tttcgccgct ggctgcgctg gccgccccgg ccaatgtgcc
     6181 gccgccgccc accattcagg aatccgccgc cgcccaggcc gagccggaag tgcgcatcat
     6241 ccagaaatcc gggcaaaaaa tcgaagaata ccggatgaac ggcaagcttt acgccatcaa
     6301 ggtcaccccg aaaatcggcc tgccttacta tctggtggac gaagacggca gcggcaaaat
     6361 gaaccgcatc gacacccacg gtgacaagct ggtggtgccg cgctgggtgc tgatgacttt
     6421 ttaagcgcca cagggatacg ctgaaaaacc gtcattcccg cacaggcggg aattcaggat
     6481 gttgatcgtg ttgaatcaat cagcgcatcc cttaag
//
LOCUS       DAOPYX010000168         6493 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_14567_length_6493_cov_8.674899, whole genome shotgun sequence.
ACCESSION   DAOPYX010000168 DAOPYX010000000
VERSION     DAOPYX010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 6493)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6493)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6493
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14567_length_6493_cov_8.674899"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            304..1155
                     /locus_tag="PKN55_09225"
     CDS             304..1155
                     /locus_tag="PKN55_09225"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNX71468.1"
                     /translation="MLADLILASYAPTAVRPPAPWCADLAAILAGPDLHAVFQPIVDF
                     ADARIVGYEGLIRGPSDSALHSPLNLFAAAEQCQQLLALERACRQVTLRRFAELRLQG
                     ALFLNISPQALLDPAFRPGETLGWMRAAGLSPEQVVIELTETRPVGDYALLREATAHY
                     RDMGFRIALDDLGEGFSNLRLWSELRPDYVKLDKYFTQGIASDAAKQQFVRSIQAIAH
                     ATGARVIAEGIETADDLACLNGLGLGLGQGYHLGRPLAQPARALDEGVTAALAAFSPC
                     HAAHPWP"
     regulatory      1160..1250
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     regulatory      1382..1499
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            1537..>2597
                     /gene="gcvT"
                     /locus_tag="PKN55_09230"
     CDS             1537..>2597
                     /gene="gcvT"
                     /locus_tag="PKN55_09230"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF001567.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HNX71469.1"
                     /translation="MADSALHTPLYAEHLAAGGKIVDFAGWALPIHYGSQLKEHETVR
                     SDAGMFDVSHMTVVDLTGADAKAYLQKLIANDVDKLGFVGKALYAGMLNPQGGVIDDL
                     IVYLTDWGYRVVVNAATRAKDLAWMQAQTAGFAVELNVRDDLSMLAIQGPNARAKVAA
                     ARPQLAELIQSLKVFQGVPVADWFVARTGYTGEDGLEIMLPDADAPAFWRELLAAGVA
                     PTGLGARDTLRLEAGMNLYGHDMDETISPLAAGMGWTIAWEPASRDFIGRAALEAERA
                     AGPAMKQVGLVLESRGVLREGMTVTVDGATGVITSGTFSPTLKLSIAIARVPAATGDV
                     ATVDLRGVATPVRVVKLPFV"
     assembly_gap    2598..2697
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2733..3119
                     /gene="gcvH"
                     /locus_tag="PKN55_09235"
     CDS             2733..3119
                     /gene="gcvH"
                     /locus_tag="PKN55_09235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017507055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HNX71470.1"
                     /translation="MSQVPAELKYNDSHEWVRLEADGTVTVGITDHAQEALGDVVFVE
                     TPAVGSELSAGSSAGVVESVKAASDIYSPIDGTVLATNPALEGEPELVNSDPYGEGWM
                     FRLQPANPAQINDMLDAAAYSDLIGA"
     gene            3189..6050
                     /gene="gcvP"
                     /locus_tag="PKN55_09240"
     CDS             3189..6050
                     /gene="gcvP"
                     /locus_tag="PKN55_09240"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012698166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HNX71471.1"
                     /translation="MTTLTELENHDGFIERHIGPSAADIDAMLGVVGAQSLDELVTQT
                     VPASILLEQDIPLPAPRAEHEALAELKALAQQNRLSKSFIGMGYADTLTPAVILRNVM
                     ENPGWYTAYTPYQAEIAQGRLEALLNYQQVIIDLTGLPLANASLLDEATAAAEAMTMA
                     RRVSTSKSNRFFVDSQIWPQTLDVLKTRAEPFGFELVLGNPADAAGADVFGALFQYPN
                     ARGEAHDLSAAIAGVKAQGGVVALAADLLALALLKSPGELGADIALGSAQRFGVPLGF
                     GGPHAAFFACRDEYKRAMPGRIIGVSIDANGGQALRMALQTREQHIRREKANSNICTS
                     QVLLANMAGLYAVYHGEAGIRRIAGYVHRLANLFAAAVRTQGGTVINQSWFDTVLVAT
                     GPRTDAVLASAEAAGYTLRRVDNNTIGVAFHEAASRADVAALAQLFTGQPADIDALDA
                     SVADAIPASLARSSRYLTHPVFNTHHSETEMLRYLKRLQNRDLALDHSMISLGSCTMK
                     LNATSEMIPVTWPEFAGLHPFAPREHAAGYLAMIDSLSEQLKAITGFDAICMQPNSGA
                     QGEYAGLLAIRRYHASRGDAQRTVCLIPKSAHGTNPATAQMMGMQVVVVACDDNGNVD
                     VADLKAKAEQYSATLACLMITYPSTHGVFEEAMRDICDTIHAHGGQVYMDGANLNALV
                     GLVQPGKIGADVSHMNLHKTFCIPHGGGGPGMGPIGLKAHLAPFMANHSVAPIANGSE
                     GQSAVSAAPFGSASILPISWMYIRMMGAAGLKRATQVALLNANYVATRLQGAFPVLYT
                     GKNGRVAHECIIDLRPLKAATGVTEVDVAKRLMDYGFHAPTMSFPVAGTIMIEPTESE
                     SKAELDRFIDALLSIRAEIAKVEAGEWAADSNPLKHAPHTQSDVISREWSRAYSRDQA
                     VFPLEWVRDNKFWPSVNRIDDVYGDRNLMCSCPGMENYQ"
BASE COUNT         1170 a         2182 c         1983 g         1058 t
ORIGIN      
        1 aatcagtcgt tcatcatcgc cgcttgaccg cgcagtttca aggcgatcag cgccaaaacc
       61 ggcaaaatca gcattatctg gcgtaaaagc cgcccacaag actctcctgg cgtcgttgca
      121 cggccttgtc gtactccctg tactgtctgc ggccgcgcgc ctagccaggc gcgcttcgct
      181 gagtttggtg agcggcacta aaaacccagt ccatacccgt cagcatgggc agagtggccg
      241 tcatcagact gtgatattgc ccggctatgc ttgccggtct ggatttaccc atggaggccg
      301 tccatgcttg ccgaccttat tctagcctca tacgcgccga cggccgtgcg cccccctgcg
      361 ccgtggtgcg cggatctggc ggcaattttg gccgggccgg atttgcacgc ggtgtttcag
      421 ccgattgtcg attttgccga cgcgcgcatt gttggctacg aagggctgat tcgcggcccg
      481 tccgacagcg cgctgcattc gccgctgaat ctgtttgcag ccgccgaaca atgccagcag
      541 ctgctggcgc tagagcgcgc ctgccggcaa gtcaccctgc gccgctttgc cgagttgcgc
      601 ctgcaaggcg cgctgtttct gaacatcagc ccgcaagcgc tgcttgaccc ggcctttcgc
      661 cccggcgaaa cgctgggctg gatgcgcgcc gccgggttaa gcccggaaca ggtggtgatc
      721 gagctgaccg aaacccggcc ggtgggcgac tatgccttgc tgcgcgaagc caccgcgcat
      781 tatcgggaca tggggtttcg cattgcgctg gacgacttgg gcgaggggtt ttccaatttg
      841 cgcctgtggt ccgagctgcg cccggactat gtcaagctgg acaaatactt tacccagggc
      901 atcgccagtg atgccgccaa gcaacagttt gtccgctcga ttcaggccat cgcccacgcc
      961 actggcgcgc gggtgattgc cgagggcatc gaaaccgccg acgacctggc ctgcctgaac
     1021 gggctgggcc ttggcctggg tcagggctac catctgggcc ggccgctggc gcagccggcg
     1081 cgcgcgctgg acgagggggt gacggcggcg ctggcggcat tttcaccttg ccatgcggcg
     1141 catccgtggc cataatccgg ccaaccatgc aggagagagc cgccccggag cggccgccga
     1201 aggcgcaagc gcacccgcaa tcgctcaggc taaaggactg catcggcctg gcttgcttca
     1261 agaagcgcta acaaaatgca gcgaagcgat cctggctagg cgtgtgaccg cagacagtac
     1321 aaacaagtac ggcaaggtca cacaacaacg ccaggagcat tttgttagtg cttcttagcg
     1381 gccgaccaac tctggagagc gccggcttac gctggacacc gaaggggcta acggcatctt
     1441 gcggcgacaa tgcgccgccg tgccggaaaa tctcaggtca tcaggacaga ggggtgtgaa
     1501 gcacattgct tatcgaccct tcaggattcc gaacccatgg ccgactccgc cctgcatacc
     1561 ccgctttacg ccgaacacct cgccgctggc ggcaagatcg ttgattttgc cggctgggcg
     1621 ctgcccattc attacggctc gcagctgaaa gaacacgaaa ccgtgcgcag cgacgccggc
     1681 atgttcgacg tctcgcacat gaccgtggtg gacctcacgg gcgccgacgc caaagcctat
     1741 ctgcaaaaac tgatcgccaa cgacgtcgac aaactgggct ttgtcggcaa ggcgctgtac
     1801 gccggcatgc tcaacccgca aggcggcgtg attgacgatc tgattgtcta tctcaccgac
     1861 tggggctacc gcgtggtggt caacgccgcc acccgcgcca aagacctggc ctggatgcaa
     1921 gcgcaaaccg ccggctttgc cgtcgaattg aacgtgcgcg atgacctctc catgctggcc
     1981 atccagggcc cgaacgcccg cgccaaggtg gccgccgccc gcccgcagct ggccgagctg
     2041 atccagtcgc tgaaggtgtt tcagggcgtg ccggtggctg actggtttgt cgcccgcacc
     2101 ggctacaccg gcgaagacgg cctggaaatc atgctgcccg acgccgacgc gccggccttc
     2161 tggcgcgagc tgctggccgc tggcgtcgcc cccactggcc ttggcgcgcg cgacaccctg
     2221 cgtctggaag ccggcatgaa tctgtacggc cacgatatgg acgaaaccat cagcccgctg
     2281 gccgccggca tgggctggac catcgcctgg gagccggcca gccgtgattt catcggccgc
     2341 gccgcgctgg aagccgaacg cgccgccggc ccggccatga agcaggtggg cctggtgctg
     2401 gaaagccgtg gcgtgctgcg cgaaggcatg accgtcacgg tcgatggcgc cactggcgtg
     2461 atcaccagcg gcaccttctc gcccaccttg aagctgtcca tcgccatcgc ccgcgtgccg
     2521 gccgccactg gcgacgtcgc cacggtggac ctgcgcggcg tggccacccc ggtgcgggtg
     2581 gtcaagttgc cgtttgtnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnaat
     2701 tgcttgattg actgaatctt caggagaaac tcatgagcca agttcccgcc gaactgaaat
     2761 acaacgacag ccacgaatgg gtgcgcctgg aagccgacgg cactgtcacc gttggcatta
     2821 ccgaccacgc ccaggaagcc ctgggtgatg tggtgtttgt ggaaaccccg gccgtgggca
     2881 gtgaactgtc cgctggctcc agcgcgggcg tggtggaatc ggtgaaagcc gcctctgaca
     2941 tctacagccc gattgacggt accgtgctgg ccaccaaccc ggcgctggaa ggcgagcccg
     3001 agctggtcaa cagcgacccg tacggcgaag gctggatgtt ccgtctgcaa ccggccaacc
     3061 cggcgcagat caacgacatg ctcgacgccg ccgcctacag cgatctgatc ggcgcctgat
     3121 agcgcgccag cgggacgccg cccttgcccg gcgcggcgtc ccgctttgtt tttggggaac
     3181 ctgcacacat gaccaccctg actgaactgg aaaaccacga cggttttatc gaacgccata
     3241 tcggcccgtc cgccgctgac atcgacgcca tgctgggcgt ggtgggcgcg caatcgctgg
     3301 acgagctggt gacgcaaacc gtgcccgcca gcattctgct ggaacaagac atcccgctgc
     3361 cggcgccgcg cgccgagcac gaagcgctgg ccgagctgaa agccctggcc cagcaaaacc
     3421 gcctgagcaa gagctttatc ggcatgggtt acgccgacac gctgaccccg gcggtgattc
     3481 tgcgcaatgt gatggaaaac cccggctggt acaccgccta caccccgtat caggccgaaa
     3541 tcgcccaagg ccgtctggaa gcgctgctga actatcagca agtgatcatc gacctgaccg
     3601 gcctgccgct ggccaacgcc tcgctgctgg acgaagccac cgccgccgcc gaagccatga
     3661 ccatggcgcg ccgggtgtcc accagcaaga gcaaccgctt ttttgtcgat agccagatct
     3721 ggccgcaaac cctggatgtg ctgaaaaccc gcgccgagcc gtttggcttt gagctggtgc
     3781 tgggcaaccc ggccgacgcc gccggcgccg atgtgtttgg cgcgctgttc caatacccga
     3841 acgcccgtgg cgaagcccac gacctgagcg cagcgattgc cggcgtcaag gcgcagggcg
     3901 gcgtggtggc cctggcggct gacctgctgg cgctggcgct gctgaagtcg ccgggcgagc
     3961 tgggcgccga cattgccctg ggttcggcgc agcgctttgg cgtgccgctg ggctttggcg
     4021 gcccgcacgc ggctttcttt gcctgccgcg acgaatacaa gcgcgccatg cccggccgca
     4081 tcatcggcgt gtcgatcgac gccaacggcg gccaggcgct gcgcatggcg ctgcaaaccc
     4141 gcgagcaaca tatccgccgc gaaaaagcca actcgaacat ctgcacctcg caagtgctgc
     4201 tggccaatat ggccggcctg tacgccgtgt accacggcga agccggcatc cgccgcatcg
     4261 ccggctatgt gcaccgcctg gccaacctgt ttgccgccgc cgtgcgcacc cagggcggca
     4321 cggtcatcaa ccaaagctgg ttcgacaccg tgctggtggc caccggcccg cgcaccgacg
     4381 ccgtgctggc cagcgccgaa gccgccggct acaccctgcg ccgggtggac aacaacacca
     4441 tcggcgtggc cttccacgaa gccgccagcc gcgccgacgt ggccgcgctg gcccagctgt
     4501 ttaccggcca gccggccgac atcgacgcgc tggacgccag cgtggccgac gccatcccgg
     4561 ccagcctggc gcgcagctcg cgctacctga cccacccggt gttcaacacc caccacagcg
     4621 aaacggaaat gctgcgctac ctcaagcgcc tgcaaaaccg cgacctggcg ctggatcact
     4681 cgatgatctc gctgggcagc tgcaccatga agctgaacgc caccagcgag atgatcccgg
     4741 tcacctggcc ggaattcgcc ggcctgcacc cgtttgcccc gcgcgaacac gccgccggct
     4801 acctggcgat gatcgacagc ctgtccgaac agctgaaagc catcaccggc tttgacgcca
     4861 tctgcatgca gcccaactcc ggcgcgcagg gcgaatacgc cggcctgctg gccatccgcc
     4921 gctatcacgc cagccgtggc gacgcccagc gcacggtgtg cctgatcccc aaatccgcgc
     4981 acggcaccaa cccggccacc gcgcagatga tgggcatgca ggtggtggtg gtggcctgcg
     5041 acgacaacgg caatgtggac gtggccgacc tgaaggccaa ggccgagcaa tacagcgcca
     5101 cgctggcctg cctgatgatc acctacccgt ccacccatgg cgtgtttgaa gaagccatgc
     5161 gcgacatctg cgacaccatc cacgcccacg gcggccaggt gtatatggac ggcgccaacc
     5221 tcaacgccct ggtgggcctg gtgcagccgg gcaagatcgg cgcggatgtg tcgcacatga
     5281 acctgcacaa gaccttctgc atcccgcacg gcggcggcgg cccgggcatg ggcccgattg
     5341 gcctgaaagc ccacctcgcc ccgttcatgg ccaaccacag cgtggcgccg atcgccaatg
     5401 gcagcgaagg ccagagcgcc gtgtcggccg cgccgtttgg cagcgcgtcg attttgccga
     5461 tttcgtggat gtacatccgc atgatgggcg ccgccggcct caagcgcgcc acccaggtgg
     5521 cgctgctgaa cgccaactac gtggccaccc gcctgcaagg cgccttcccg gtgctgtaca
     5581 ccggcaagaa cggccgcgtg gcgcacgagt gcatcatcga cctgcgcccg ctgaaggccg
     5641 ccaccggcgt gaccgaagtg gacgtggcca agcggctgat ggactacggc ttccacgccc
     5701 cgaccatgag cttcccggtg gccggcacca tcatgatcga accgaccgaa agcgaatcca
     5761 aggctgaact cgaccgcttt atcgacgcgc tgctgagcat ccgcgccgaa atcgccaagg
     5821 tggaagccgg cgaatgggcc gctgacagca acccgctcaa gcacgccccg cacacgcaaa
     5881 gcgatgtgat cagccgcgag tggagccgtg cgtacagccg tgatcaggcg gtgttcccgc
     5941 tggaatgggt gcgcgacaac aagttctggc cgtcggtcaa ccgcattgac gatgtgtatg
     6001 gcgaccggaa tctgatgtgc tcgtgcccgg gcatggagaa ctatcagtaa gcttggcgcg
     6061 actgacgcaa tcctcctggc gtgctggcgc gaccttgccg cacggctcgc cagggatcgc
     6121 tgcgctggat tgtgcgcgct gcgtttgagc gtgtgacgcc aaaaaaaccg cccaccggcc
     6181 attgccggat gggcggtttt tttatctgca cgccgcgcat cgcttacaga ggtacagggc
     6241 cgacgttttg cacagagtgt gcaccacacc ccggcgcttg gcaggcgatc aaaatcgggc
     6301 gttggcgcgc cgattttttc catcaaacac catgaagagg ctcatccatg aaagcacatc
     6361 attctttcct gcactggcca cgcgccgtcg ccagcctggg tttactggcc ctggatctgt
     6421 ctgccccggc actggccgcc ccctgccgcg acatcgccac ggtgcaacaa agcgccaatg
     6481 ccgaccagaa cac
//
LOCUS       DAOPYX010000169         6482 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_14602_length_6482_cov_8.416680, whole genome shotgun sequence.
ACCESSION   DAOPYX010000169 DAOPYX010000000
VERSION     DAOPYX010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 6482)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6482)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6482
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14602_length_6482_cov_8.416680"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            290..553
                     /locus_tag="PKN55_09245"
     CDS             290..553
                     /locus_tag="PKN55_09245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX71472.1"
                     /translation="MSSHVTPSPANPRHSASARAVLAELLHHPLAGHWPAEFQALLCV
                     NAARLRGEQGQHAQAGRWLHKARALSQCAVTLREIEQLALMPQ"
     gene            complement(579..1745)
                     /locus_tag="PKN55_09250"
     CDS             complement(579..1745)
                     /locus_tag="PKN55_09250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014086453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HNX71473.1"
                     /translation="MKLPEHSELIVVGGGPVGATLALLAARAGRQVTLLEARAHDAGR
                     RDDPRVLALSHASQQALLAAGGWPDSLAATAIDTVHISQQGSFGRTLIQRDDLQLPHL
                     GYTVSYPDLNQALDAALAASSVRVIHGAEVQGLHSLSAYAQVRFAHQGHTRQMTGRLV
                     ALAEGGALAAQLPGMRRHEHDYQQSALICQVDVSVPHQGMAFERFAVDGPLALLPCGD
                     AMMLVWTRQGAAAEALTACDDADFIAQLQAAIGDRLGAITGVGARACVPLRLKFALRT
                     VSGRVALVGNAAQTMHPVAAQGLNLGLRDAVGLVDSLAGARDIGEAAVLAGFARQRQL
                     DRRAVTGFTHSLIQLFDRHDPLLNAARGLGLTLLDNIAPLRRAFAGHLVFGVGG"
     gene            complement(1742..3046)
                     /gene="pepP"
                     /locus_tag="PKN55_09255"
     CDS             complement(1742..3046)
                     /gene="pepP"
                     /locus_tag="PKN55_09255"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018747245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HNX71474.1"
                     /translation="MSTAHYVERRRRLMAELGAGVVILPTAAHAVRNADTHYPYRADS
                     SFYYLTGFDEPEAVLVLLPAEGQSILFCREKDLEREIWDGFRHGPDGARERFAFDQAY
                     PLSELDAHLPKWLGNQPTVHYTLGRDSGWDQRVLGWLDAVRGMARSGVTTPPAIHDVS
                     LAIAEMRLFKDAHEIQLLRRAGEISAEAHVRAMRACQPGLYEYQVEADILHTFVRHGA
                     RSPAYESIVAGGANACVLHYVGNRDVLRDGDLLLIDAGCEYQGYAGDITRTFPVNGRF
                     SGEQRAVYEVVLAAQLAAIAAIRPGVAWNVPNDAALSVLVQGLIDLGLLHGTVDGNIE
                     SEAYRQFYMHRIGHWLGLDVHDVGRYKLDGQWRALQAGMVTTVEPGLYLRAAANVPER
                     FAGIGIRIEDDVLVSAEGGVVLTDGAPKTVADIEALMAEARA"
     gene            complement(3218..3457)
                     /locus_tag="PKN55_09260"
     CDS             complement(3218..3457)
                     /locus_tag="PKN55_09260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX71475.1"
                     /translation="MTMGSALTWWLAALLVAMLEVSSGSFYLLAVALGLASVGLGLFL
                     GLGLPLALSGGSGHIVLRMVDDDTGQEVQLAWLGV"
     gene            complement(3540..3803)
                     /locus_tag="PKN55_09265"
     CDS             complement(3540..3803)
                     /locus_tag="PKN55_09265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018747840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HNX71476.1"
                     /translation="MVQPDKSALLARLARIEGQVRGVARMVEEDRYCVDILTQLAAAR
                     AALNGVAMQLLTHHAHGCVQQAIQSGEGEAALDELMATVKKLL"
     gene            complement(3847..4056)
                     /locus_tag="PKN55_09270"
     CDS             complement(3847..4056)
                     /locus_tag="PKN55_09270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009117844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-associated domain-containing
                     protein"
                     /protein_id="HNX71477.1"
                     /translation="METLTLNIDGMTCMGCVANITRVLTALPGVDSAVVNKDAANAVI
                     TFNPAQQSAATLSQAIEDAGFDVRG"
     gene            complement(4104..6461)
                     /locus_tag="PKN55_09275"
     CDS             complement(4104..6461)
                     /locus_tag="PKN55_09275"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008953078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HNX71478.1"
                     /translation="MTPPILTLPIAGMTCAACAVRLEKVLKRLPDVDAEVNFATASAR
                     LTSRTVQPPDLAAAMAAVARAGFQVPLSRQRLTLEGMTCAACAQRIEKVLNRLPGVHA
                     SVNFATASAEVDYPAGVVSSAQLLDTVRRAGYQAQLPRTDAPVTAAGEQDHTGRWLLA
                     STLLALPFVAEMLAMLAGRHGLLPVAWQWALATPVQFLVGWRFYRGAWLSLRGGGANM
                     DVLVALGTSMAYLLSVWTALTAPHHGHVYFEASVSVITLVLLGKWLEQRARRQTAGAI
                     EALLKLAPTTARVERDGAVQDVPVAEVRVGEVVWVREGESAPVDGVVIDGQGSVDESL
                     LTGESAPVSKGVDSPVYAATRNLQGVLRLRATGVGADTQLAAIIRQVGEAQGSKAPIQ
                     RLADVISGYFVPAVLAIAVATLLAVGASSGDWERAIMQAVAVLVIACPCALGLATPAA
                     VMVGVGLGARHGVLFRHATALEHAGRLTVLAVDKTGTLTEGRPQLTEVIALAADNREQ
                     ALALAASLEAGSEHPLAAAILRAAQDAQLGLQPVEAFSVSVGQGVSGVINGRALRLGV
                     PAWVSPTLAEHPQVTRLTEAGHTVAALADETQVLALLALSDAIRPSAAPTIARLRAMG
                     VEVHMLTGDNPAAAARVAKALDIRCWQAGVRPDGKARAIAELQARGERVGMAGDGVND
                     APALAQADVSLAMGGGSHVAIETADITLMHGDLLAAADAIDLSRRTLAKIRQNLFFAF
                     IYNILGIPLAALGGLNPVIAGAAMAMSSVSVVSNALLLRRWRPGR"
BASE COUNT         1058 a         2241 c         2181 g         1002 t
ORIGIN      
        1 ttggcggcgc gaaacgcggc atctgtgcgc cacggattca cgccgtgaat aaagtatttc
       61 acgcccgtgc caagtggttt agtcgcacgc ccgtggtgag ctggccgggc cacacggcaa
      121 gctaggctgc cattcccccg gccggtgctg agcgccgctg accaaacccc gttggcgtga
      181 ttgggtgacc tggccacact ggcttgctca ccctgctggc cgcgctgagt ttggtgagcc
      241 gtgaacatcg gtgcgtgact gaaatcagcc cgttgccaag gagcgcatca tgagcagcca
      301 tgttacccca tcccctgcca acccccgcca cagcgcctcg gcgcgcgccg tgctggccga
      361 gctgctgcac cacccgctgg ccggacattg gccggccgag tttcaggcgc tactgtgcgt
      421 gaatgctgcg cgtctgcgcg gcgaacaggg ccagcatgcc caggccgggc gctggctgca
      481 caaggcacgg gcgctcagcc agtgcgctgt caccttgcgc gagatagagc aactggccct
      541 gatgccgcag taagcgcggc tgacaaaaca caggtgagtc agccgcccac accaaacacc
      601 agatggccag caaaggcgcg gcgcagcggg gcgatattgt ccagcagggt gagccctagt
      661 ccgcgcgcgg cgttcagcag agggtcgtgg cggtcaaaca gctgaatcag gctgtgggta
      721 aagccggtga cggcgcggcg atccagctgg cgttggcggg caaagccggc cagcaccgcc
      781 gcctcgccga tgtcgcgcgc gccggccagg ctgtccacca ggccgacggc gtcgcgcagg
      841 cccaggttca gcccttgcgc cgccaccggg tgcatggttt gcgcggcgtt gcccaccagc
      901 gccacccggc cgctgaccgt gcgcagggca aatttcagcc gcagcggcac acaggcgcgg
      961 gcgcccaccc cggtgatggc gcccagccgg tcgccgatgg cggcctgcaa ctgggcgata
     1021 aaatcggcgt cgtcgcaggc ggtcagcgcc tcggcggcgg cgccttgccg cgtccacacc
     1081 agcatcatgg cgtcgccgca gggcaacagc gccagcgggc catccacggc aaagcgctca
     1141 aacgccatgc cctgatgcgg cacgctcacg tccacctggc agatcagcgc cgattgctgg
     1201 tagtcatgct cgtggcggcg catgccgggc aattgggcgg ccagcgcgcc gccttcagcc
     1261 agcgccacca gccggccggt catctgccgg gtgtggccct ggtgggcaaa gcgcacttgc
     1321 gcataggccg acaaggaatg cagcccctgg acttctgcgc cgtgaatcac ccgcaccgac
     1381 gaggccgcca gcgcggcgtc cagcgcctga ttcaaatccg ggtaagacac ggtgtagccc
     1441 agatgcggca gctggaggtc gtcgcgctga atcagcgtgc ggccaaagct gccttgctgg
     1501 ctgatgtgca cggtgtcgat ggcggtggcg gccaggctgt ccggccagcc gccagccgcc
     1561 agcagcgctt gctggctggc gtgcgacagc gccagcacgc gcgggtcgtc gcggcggccg
     1621 gcgtcgtggg cgcgcgcttc cagcagggtg acttgccggc cggcgcgcgc cgccagcaag
     1681 gccagggtgg cgcccaccgg gccgccgccc accacgatca gttcgctgtg ttcgggcagc
     1741 ttcatgcgcg cgcctcggcc atcagcgctt cgatatcggc caccgtcttg ggcgcgccgt
     1801 cggtcagcac cacgccgccc tcggcgctca ccagcacatc gtcttcaata cggatgccaa
     1861 tgccggcaaa gcgctcgggc acattggccg ccgcgcgcag atacagcccc ggctcgacgg
     1921 tggtcaccat gccggcttgc aaagcgcgcc attggccgtc cagcttgtag cggccaacgt
     1981 cgtgcacgtc caggcccagc cagtggccga tgcggtgcat gtaaaactgc cggtaggctt
     2041 ctgattcgat attgccgtcc accgtgccgt gcagcaggcc caggtcgatc agcccttgca
     2101 ccagcacgct taaggcggcg tcgttgggca cattccaggc cacgccgggg cggatggcgg
     2161 caattgccgc caactgcgcg gccagcacca cctcgtacac cgcgcgctgt tcgccgctga
     2221 agcggccgtt caccggaaag gtgcgggtaa tgtcgcccgc gtagccctgg tattcgcaac
     2281 cggcgtcgat caacagcaag tcgccatcgc gcagcacatc gcggttgccc acatagtgca
     2341 gcacgcaggc attggcgccg ccagcgacga tgctttcgta ggcggggctg cgcgcgccgt
     2401 ggcggacaaa ggtgtgcagg atgtcggcct ctacctggta ttcatacagg ccaggctggc
     2461 aggcgcgcat ggcgcgcaca tgcgcctcgg cggagatttc accggcgcgg cgcagcagct
     2521 ggatttcgtg ggcgtctttg aacaggcgca tctcggcaat cgccagcgac acatcgtgaa
     2581 tggccggcgg cgtggtgacg ccgctgcgcg ccatgccgcg cacggcgtcc agccagccca
     2641 atacccgttg atcccagccg ctgtcgcggc ccagggtgta gtgcacggtg ggctggttgc
     2701 ccagccattt gggcagatgc gcatccagtt cgctgagcgg gtaggcctgg tcaaaggcaa
     2761 agcgctcgcg cgcgccgtcc gggccgtggc gaaaaccgtc ccagatttcc cgctccaggt
     2821 ctttttcccg gcagaacagg atggactgcc cttccgccgg cagcagcacc agcacggctt
     2881 ccggctcgtc aaagccggtg aggtaataaa aactgctgtc ggcgcgatag ggatagtggg
     2941 tgtcggcatt gcgcacagcg tgcgcggcgg taggcaaaat gacaacgcca gcgcccagct
     3001 cggccatcag gcggcggcgg cgttcgacgt aatgggctgt ggacatgaac aggattcccg
     3061 acaatggcac gaggccggcc agggagatgg ccggatacag aatgcgccag tgtacaccgg
     3121 cggcgcggcg atgatgaagc cagccagata agcgccgcgc acaaaaccct cctggcgtgg
     3181 ttgctcgatc ttgccgtacg acttgtactg tctgcggtca cacgcctagc caggccagtt
     3241 gcacctcttg cccagtgtca tcatccacca tgcggagcac gatgtgcccg ctgccgccag
     3301 acagggccag cggcaagccc aggcccagaa acagtcccag tccgacgctg gccagcccca
     3361 gtgccaccgc cagcaggtaa aacgagccac tggaaacctc cagcatggcc accagcaggg
     3421 cagccagcca ccacgtcagc gccgagccca ttgtcatgat tttcgtccga aaatacaaag
     3481 atcatagcga gggcgtatgt ctcgggaaag cggagaccgg tcaaaaaacg gctgtccgct
     3541 tacagcagtt ttttgaccgt ggccatcagc tcgtccagcg ccgcctcgcc ttcgccgctc
     3601 tggatggcct gttgcacgca gccgtgggcg tggtgggtga gcaactgcat ggccacgccg
     3661 ttcagcgcgg cgcgggcggc ggccagctgg gtgaggatgt ccacacagta gcggtcttct
     3721 tccaccatgc gcgccacgcc gcgcacctgg ccttcaatgc gcgccagccg cgccagcagg
     3781 gcggatttgt ctggctgcac cacccgcttg tcgctgcccg gcgcgctgtc ttcgctgtgg
     3841 cagcactcag ccacggacgt caaacccggc gtcttcgatg gcctgactca gcgtggcggc
     3901 gctttgctgg gccgggttga aggtgatcac ggcgttggcg gcgtctttgt tgaccaccgc
     3961 gctgtccacg ccgggcaggg cggtcagcac gcgggtgata ttggccacgc agcccatgca
     4021 ggtcatgccg tcgatattga gggtgagggt ttccattgtg ttgctcctgt gggtgaaaaa
     4081 atctgtgtcg cgctcagtgt tgctcagcgc cctggccgcc agcggcgcag caacaaggca
     4141 ttgctcacca ccgacactga gctcatcgcc atggccgcgc cggcaatcac cgggttcagc
     4201 ccgcccagtg ctgccagcgg aatgcccagg atattgtaaa taaaggcaaa aaacaggttc
     4261 tggcgaattt tggccagggt gcggcgcgat aaatcaatcg catcggcggc ggccagcaga
     4321 tcgccgtgca tcagggtgat gtcggcggtt tcaatcgcca catggctgcc gccgcccatg
     4381 gccaggctga cgtcggcctg cgccagcgcc ggggcgtcgt tgacgccgtc gccggccatg
     4441 cccacccgct cgccgcgcgc ttgcagttcg gcaatcgcgc gcgccttgcc gtccgggcgc
     4501 acgccggcct gccagcagcg aatatccagc gccttggcca ctcgcgcggc ggcggccggg
     4561 ttgtcgccgg tcagcatatg cacttccacg cccatggcgc gcagccgggc gatggtgggc
     4621 gcggcggacg ggcggatggc gtcggatagc gccagcagcg ccaagacttg agtttcatcc
     4681 gccagcgccg ccacggtgtg gccggcctcg gtcagccggg tgacctgcgg gtgttcggcc
     4741 agggttgggc tgacccaggc aggcacgccc agtcgcagcg cgcgaccgtt gatcaccccg
     4801 gatacaccct ggccgacgct gacgctgaat gcctcgacgg gctgcaaacc cagctgggcg
     4861 tcttgcgcgg cgcgcaggat ggcggcggcc agcgggtgtt cgctgccggc ttccaggctg
     4921 gcggccagcg ccagcgcctg ttcgcggttg tcggcggcca gggcgatgac ttcggtcagc
     4981 tgcgggcggc cttcggtgag cgtgccggtt ttgtccaccg ccagcacggt cagccggccg
     5041 gcgtgctcca gcgcggtggc gtggcgaaac agcacgccat ggcgggcgcc caggccgacg
     5101 cccaccatca ccgccgccgg ggtggccagg cccagcgcgc acgggcaggc aatcaccagc
     5161 accgccaccg cctgcatgat ggcgcgttcc cagtcgccgc tgctggcccc caccgccagc
     5221 agggtggcca cggcaatggc cagcaccgcc ggcacaaaat acccggaaat cacgtccgcc
     5281 agccgctgga tcggcgcttt cgagccttgc gcctcgccca cctggcggat gatggcggcc
     5341 agttgggtgt cggcgcccac gccagtggcg cgcaggcgca gcacgccttg cagattgcgg
     5401 gtggcggcgt acaccgggct gtccacgccc ttgctgaccg gggcgctttc gccggtcagc
     5461 aggctttcgt ccacgctgcc ctggccgtcg atcaccacgc cgtccacggg cgcgctttcg
     5521 ccttcgcgca cccacaccac ctcgcccacg cgcacctcgg ccaccggcac atcctgcacc
     5581 gcgccgtcgc gctccacccg cgccgtggtc ggcgccagct tgagcagggc ttcgatggcc
     5641 ccggcggttt gccggcgcgc gcgctgttcc agccacttgc ccagcagcac cagggtgatc
     5701 accgacacgc tggcttcaaa gtacacatgg ccatgatgcg gcgcggtcag cgccgtccac
     5761 acgctcagca gataggccat gctggtgccc agcgccacca gcacatccat attggcgccg
     5821 ccgccgcgca ggctgagcca ggcgccgcga taaaagcgcc agcccaccaa aaattgcaca
     5881 ggcgtggcca gcgcccactg ccaggccacc ggcaacagac catggcggcc ggcgagcatg
     5941 gccagcattt ccgccacaaa cggcagcgcc agcagggtgg acgccagcag ccagcggccg
     6001 gtgtggtctt gctcgccggc cgctgtgacg ggggcgtcgg tgcgcggcag ctgcgcctga
     6061 taaccggcgc ggcgcaccgt gtccagcagc tgcgcgctgc tgaccacgcc ggccgggtaa
     6121 tccacctcgg cgctggcggt ggcgaagttg acgctggcgt gcacgccagg cagacgattg
     6181 agcacttttt cgatgcgctg ggcgcaggcg gcgcaagtca tgccctccag cgtcagccgc
     6241 tggcggctga gcggcacctg aaaaccggcg cgcgccaccg ccgccatggc ggcggcaagg
     6301 tcgggcggct ggaccgtgcg tgaggtgagc cgcgcgctgg cggtggcaaa gttgacctcg
     6361 gcgtctacat ccggcaggcg cttgagcact ttttccagcc gaaccgcgca ggcggcgcag
     6421 gtcatgccgg cgattggcaa ggtgaggatg gggggcgtca tggcgtggct ttcttatacc
     6481 ct
//
LOCUS       DAOPYX010000170         6465 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_14657_length_6465_cov_11.591420, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000170 DAOPYX010000000
VERSION     DAOPYX010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 6465)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6465)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6465
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14657_length_6465_cov_11.591420"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2702)
                     /gene="rpoB"
                     /locus_tag="PKN55_09280"
     CDS             complement(<1..2702)
                     /gene="rpoB"
                     /locus_tag="PKN55_09280"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HNX71479.1"
                     /translation="MSYSFTEKKRLRKSFAKRAGVLDVPFLLATQIDSYTEFLQLGVP
                     FDKRESIGLEAAFRSIFPIHSHNGYARLDFVHYVLGDPPFDVPECQLRGITFAAPLRA
                     RIRLTILDKESSKPVVKEVRENEVYMGEIPLMTGNGSFIINGTERVIVSQLHRSPGVF
                     FEHDRGKTHSSGKLLFSARVIPYRGSWLDFEFDPKDLLYFRIDRRRKMPVSILMKALG
                     YSEEQILAEFFSFDTFYLTEDGVYMELVADRLKGEVAKFDIVTADGKTIVQKDKRITA
                     KHIRDIQTSGLTRVEVPADMLVGRVLAKNVVNPETGELLVRANDEITEELLAKLDICD
                     VRQVDVLYTNELDQGAFISQTMRSDDIQDQLQAKVAIYRMMRPGEPPTEEAVEALFQR
                     LFFNEDAYDLSRVGRMKFNRRIGRDELVGSGTLSHEDIVAVIKILVDLRNGRGEVDDI
                     DHLGNRRVRSVGELAENQFRAGLVRVERAVKERLNQAESDNLMPHDLINAKPVSAAIK
                     EFFGSSQLSQFMDQTNPLSEVTHKRRVSALGPGGLTRERAGFEVRDVHPTHYGRVCPI
                     ETPEGPNIGLINSLAIYARTNEYGFLETPYRKVVDSKVTNEIHYLSAIEEGRYVIAQA
                     NAELDDEGRLIDELVTCREKGETILAMPDRVQYMDVAPSQVVSVAASLIPFLEHDDAN
                     RALMGANMQRQAVPCLRAEKPLVGTGIERAVATDSGTTVVARRGGLVEYVDANRVVIR
                     VNDEEALAGEVGVDIYNMTKYTRSNQNTNINQRPIVKVGDHVARGDVLADGASTDLGE
                     LALGQNMTIAFMPWNGYNFEDSILISERVVADDRYTSIHIEELQVVARDTKLGPEEIT
                     RDIPNLSERMQGRLDESGIVYIGAEVEAGDVLVGKVTPKGETQLT"
     gene            complement(2891..3262)
                     /gene="rplL"
                     /locus_tag="PKN55_09285"
     CDS             complement(2891..3262)
                     /gene="rplL"
                     /locus_tag="PKN55_09285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HNX71480.1"
                     /translation="MAITKEDILEAVGNLTVMELNDLVKAFEEKFGVSAAAMAVAAPG
                     AGAAPAAEEKTEFDVVLQAAGDNKVGVIKVVRAVTGLGLKEAKDLVDGAPKTVKEGLS
                     KADAEAMVKQLVEAGAKAEMK"
     gene            complement(3310..3831)
                     /gene="rplJ"
                     /locus_tag="PKN55_09290"
     CDS             complement(3310..3831)
                     /gene="rplJ"
                     /locus_tag="PKN55_09290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011137742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HNX71481.1"
                     /translation="MSLNLEDKKAVVAEVAAQVAGAQTLVIAEYRGIAVSSMTKLRAK
                     AREQGVYLRVLKNTLVRRAVADTPFAPLADQMVGPLVYAVSEDPVAAAKVLSDFAKTD
                     DKIIVKAGVYDGKLLDKAAVAQLASIPGREQLLAMLLGIMQAPVSGFARALAALAEKQ
                     AGGAEAPAEEAAA"
     gene            complement(4115..4816)
                     /gene="rplA"
                     /locus_tag="PKN55_09295"
     CDS             complement(4115..4816)
                     /gene="rplA"
                     /locus_tag="PKN55_09295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012695733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HNX71482.1"
                     /translation="MAKVSKRLAALKATVDRNKLYAVEEALTLVKGAATAKFDESVDV
                     AVNLGIDPRKSDQVVRGSVVLPRGTGKSVRVAVFAQGANAEAAKAAGAEIVGFEDLAD
                     QIKGGMMDFDVVIATPDAMRIVGQLGQILGPRGLMPNPKVGTVTPNVTEAVKNAKAGQ
                     VQYRTDKSGIVHATIGRASFEVDALRTNLAALVEALVKAKPASSKGVYLKKIAVSSTM
                     GIGVRVDQLSLSEQA"
     gene            complement(4816..5247)
                     /gene="rplK"
                     /locus_tag="PKN55_09300"
     CDS             complement(4816..5247)
                     /gene="rplK"
                     /locus_tag="PKN55_09300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008343796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HNX71483.1"
                     /translation="MAKKIVGYIKLQVPAGKANPSPPIGPALGQRGLNIMEFCKAFNA
                     QTQGVEPGLPIPVVITAYADKSFTFIMKTPPATILIKKAAGVQKGSPKPHTDKVGKLN
                     RAQLEEIAKTKQPDLTAADMDAAVRTIAGSARSMGIEVEGV"
     gene            complement(5358..5891)
                     /gene="nusG"
                     /locus_tag="PKN55_09305"
     CDS             complement(5358..5891)
                     /gene="nusG"
                     /locus_tag="PKN55_09305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003760509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HNX71484.1"
                     /translation="MAMRWYVVHAYSGFEKSVQKALKERIERENMQDRFGKILVPVEE
                     VVDMKNGKRSLTERKFFPGYVLVEMEMTDESWHLVKSTPKVTGFVGGTANRPAPIPQK
                     EVDAIMQQMQEGVEKPKPKILFEVGERVRVIDGPFTDFNGAVDEVNYERNKLRVSVQI
                     FGRDTPVELEFNQVEKL"
     gene            complement(5898..6251)
                     /gene="secE"
                     /locus_tag="PKN55_09310"
     CDS             complement(5898..6251)
                     /gene="secE"
                     /locus_tag="PKN55_09310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HNX71485.1"
                     /translation="MESQDKLKLVGALLCVVAGVAGFYLIPEGQGVLRVLAVLAGVAA
                     AGGVVWLSQPGKDFVLYAQDSVAEAKKVVWPTRKEATQMTVMVFVFVFVLALFMWLVD
                     SGLSWLFYDILLKRG"
     gene            complement(6316..6391)
                     /locus_tag="PKN55_09315"
     tRNA            complement(6316..6391)
                     /locus_tag="PKN55_09315"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6356..6358),aa:Trp,seq:cca)
BASE COUNT         1232 a         1975 c         1895 g         1363 t
ORIGIN      
        1 gtcagctggg tttcgccctt cggcgtcacc ttgcccacca gcacgtcgcc ggcttccact
       61 tcggcgccga tgtagacaat gccggattcg tccagacggc cttgcatgcg ctcggacagg
      121 ttcggaatat cacgggtgat ttcttccggc cccagcttgg tgtcgcgcgc caccacttgc
      181 agttcttcga tgtgaatcga ggtgtagcgg tcatcggcca ccacgcgctc ggaaatcagg
      241 atcgaatctt cgaagttgta gccgttccac ggcatgaagg cgatggtcat gttctgaccc
      301 agcgccagtt cgcccaagtc agtcgacgcg ccatcggcca gcacgtcgcc acgggcgaca
      361 tggtcaccca ccttgacgat ggggcgctgg ttgatgttgg tgttctggtt ggagcgggtg
      421 tacttggtca tattgtagat gtccacaccc acttcaccgg ccagcgcttc ctcgtcattg
      481 acgcggatca ccacccggtt ggcgtcgaca tactcgacca gaccgccacg acgggccacc
      541 acggtggtgc ccgagtcagt ggccaccgca cgctcgatgc cggtgccgac cagcggcttt
      601 tctgcgcgca ggcaaggcac ggcctgacgc tgcatgttgg cgcccatcag tgcacggttg
      661 gcgtcatcgt gctccaggaa cggaatcagc gaagccgcca ccgacaccac ctggctcggg
      721 gccacgtcca tgtactgcac gcggtccggc atggccagaa tggtttcgcc cttttcacgg
      781 caggtgacca gttcgtcaat caggcggcct tcgtcgtcca gctcggcgtt ggcctgagca
      841 atcacatagc ggccttcttc gatggccgac aggtaatgga tttcgttggt gaccttgctg
      901 tccaccacct tgcggtacgg ggtttccagg aaaccgtact cattggtgcg ggcgtaaatg
      961 gccagcgagt tgatcaggcc gatgttcggg ccttccggcg tttcaatcgg gcacacgcgg
     1021 ccgtagtggg tcgggtgcac gtcacgcact tcaaagccag cgcgttcgcg ggtcaaaccg
     1081 cccgggccca gggccgacac gcggcgcttg tgggtgactt ccgacagcgg gttggtctgg
     1141 tccatgaact gcgacaattg actggagccg aaaaactcct tgatggcggc gctgaccggc
     1201 ttggcgttga tcaggtcgtg cggcatcagg ttgtcggatt cagcctggtt caggcgttcc
     1261 ttgacggcgc gctcaacgcg caccagaccg gcgcggaatt ggttctcggc cagttcgccc
     1321 accgaacgca cgcggcggtt gcccaggtga tcgatgtcat cgacttcgcc acggccattg
     1381 cgcaggtcca ccagaatctt gatcacggcg acaatgtctt cgtgggacag cgtaccggaa
     1441 cccaccagct catcacggcc gatgcggcgg ttgaacttca tgcggccgac gcgcgacagg
     1501 tcataggcgt cttcgttgaa gaacaggcgc tggaacagtg cttccacggc ttcttcggtc
     1561 ggcggctcgc ccgggcgcat catgcgatag atggcgacct tggcttgcag ctggtcctgg
     1621 atgtcgtcac tgcgcatggt ctgcgaaatg aaggcgccct ggtccagttc gttggtgtac
     1681 agcacgtcca cctgacgcac gtcgcagatg tccagcttgg ccagcagctc ttcggtgatt
     1741 tcgtcgttgg cgcgcaccag cagctcgccg gtttccgggt tgaccacgtt cttggccagc
     1801 acacggccca ccagcatatc ggccggcact tcgacgcggg tcaggcccga ggtctggatg
     1861 tcgcggatat gcttggcggt gatgcgcttg tctttctgca cgatggtctt gccatcggca
     1921 gtgacgatgt caaacttggc cacttcgccc ttcaggcgat cggccaccag ctccatgtaa
     1981 acaccgtctt cggtcaggta gaaggtgtcg aagctgaaga attcggccag gatttgctct
     2041 tcgctatagc ccagcgcctt catcaggatg gacaccggca tcttgcgacg acggtcgata
     2101 cggaagtaca gcagatcctt cgggtcgaat tcgaagtcca gccaggagcc gcggtaagga
     2161 atcacgcggg cggagaacag cagcttgccc gagctgtggg tcttgccacg gtcgtgctcg
     2221 aagaacacgc ccggcgaacg gtgcagctgg gagacgataa cgcgctcggt gccattgatg
     2281 atgaacgagc cattgccggt catcagcggg atttcgccca tgtacacttc gttttcgcgc
     2341 acttccttga ccaccggctt ggacgattct ttgtccagga tggtcaggcg gatgcgggcg
     2401 cgcagcggcg cagcaaaggt gatgccgcgc agttggcatt ccggcacgtc aaacggcggg
     2461 tcgcccagca catagtggac gaaatccagg cgagcgtaac cgttgtggct gtgaatcggg
     2521 aagatggaac gaaacgccgc ttcgagaccg atgctctcgc gtttgtcgaa cggcacgccc
     2581 aactggagga actcggtata cgaatcgatt tgtgtcgcca acaggaaggg gacgtcgaga
     2641 acaccggcac gtttcgcaaa gctcttgcga agacgttttt tctcagtaaa cgaataactc
     2701 atagagtctc caacgaggtg gggtggcgca agaaaacaaa actcaacgcg ccagcgctga
     2761 cgggctgact tttgttttct tttgaagtgc ggggatgtgc ccgccgccag tctttaaaga
     2821 acacactaca caagcgcaac aaggctggca gatttctccg ccagcctttg ccgcatcgtc
     2881 gtgacgacga ttacttcatt tcggccttgg cgccggcttc caccagctgc ttcaccatgg
     2941 cttcggcgtc agccttggac aggccttcct tcacggtttt cggagcgccg tccaccaggt
     3001 ccttggcttc cttcagaccc aggccggtca cagcgcgcac aaccttgatc acgccaacct
     3061 tgttgtcgcc ggcggcttgc agcacgacgt cgaactcggt cttctcttca gcagccggag
     3121 cagcgccagc gcccggggca gccacggcca tggcggcagc ggacacgccg aacttttctt
     3181 cgaacgcctt gaccaggtcg ttcagttcca tcacggtcag gttgccaacg gcttcgagga
     3241 tgtcttcttt ggtgattgcc atgtttatta ctctctagaa atcagttaaa tctttaatga
     3301 atgatcggtt caggcagcgg cttcttcggc cggggcttct gcgccaccgg cttgcttctc
     3361 ggccagggct gccagtgcgc gggcaaagcc ggacaccggg gcttgcatga tgcccagcag
     3421 catggccagc agctgttcgc ggcccgggat ggacgcgagt tgcgccacag cggccttgtc
     3481 cagcagcttg ccatcgtaca cgccagcctt gacgatgatt ttgtcatcgg tcttggcgaa
     3541 gtcgctcagc actttggcgg cagcaaccgg atcttccgac acggcgtaca ccagcgggcc
     3601 aaccatttgg tctgccagcg gagcgaacgg ggtgtcagcc acagcacggc gcaccagcgt
     3661 gttcttcagc acgcgcaggt acacgccctg ctcgcgggcc ttggcgcgca gcttggtcat
     3721 gctgctcacc gcgataccgc gatattcggc gataaccagg gtctgggcgc ctgccacttg
     3781 ggcagccact tcggccacta ccgccttttt atcttcaaga ttgagactca aggtctacct
     3841 cctgttgttg aggaatgggt cgggcaatgc ccttcccccc acgataacgg cgacctgatt
     3901 tcaggagttg tctcaacaat gccggccagt gagtggccag caaggacaga aacgacctgc
     3961 attcgggtgc accatctgcg caggatgcat cgacgtgatg cggattaagc ctttcggcgc
     4021 ctgcggtctt tgacaatctg acgcagcatg ctgcatcagc ccaaagcaaa cgatacgggc
     4081 aacgccttac agaaggcgct gcccgtcaaa tacatcaagc ctgctcggag agcgacagct
     4141 gatcgacgcg aacgccaatg cccatggtgc tggagacggc gatcttcttc aggtaaacac
     4201 ccttggaaga ggccggcttg gccttcacca gtgcttccac cagcgcagcc aggttggtgc
     4261 gcagggcgtc cacttcaaac gaagcgcggc cgatggtggc gtgcacgata ccggacttgt
     4321 cagtacggta ttgcacttgg ccagccttgg cgtttttcac ggcttcggtg acgttcgggg
     4381 tcacggtgcc aaccttcggg ttcggcatca ggccgcgcgg gcccaggatt tgacccagtt
     4441 gaccgacgat gcgcatggcg tccggggtgg cgatcaccac gtcgaagtcc atcatgccgc
     4501 ccttgatctg gtcggccaga tcttcgaagc ccacgatttc agcgccggca gccttggcgg
     4561 cctcggcgtt ggcgccttga gcaaacacgg ccacgcgcac gctcttgccg gtgccgcgcg
     4621 gcagcacgac ggagccgcgc accacttggt cggatttacg cgggtcgatg cccaggttga
     4681 cggcgacgtc aacggattcg tcgaatttgg cggtggcggc gcccttgacc agggtcaggg
     4741 cttcttccac ggcgtacagc ttgttgcggt ccacggttgc cttcagggca gccagacgct
     4801 tggaaacctt agccattaca cgccctccac ttcaatgccc atgctgcgag cggaaccggc
     4861 gatggtgcgc acggcggcgt ccatgtcagc agcagtcagg tcgggttgct tggtcttggc
     4921 gatttcttcc agctgggcgc ggttcagctt gcccaccttg tcggtgtgcg gcttcgggct
     4981 gcccttttgc acgcctgcgg ccttcttgat cagaatggtc gccggcgggg tcttcatgat
     5041 gaaggtgaac gacttgtccg cgtaagcggt gatcaccacc ggaatcggca gacccggctc
     5101 aacaccttga gtctgggcgt tgaaggcctt gcagaattcc atgatgttca gaccgcgctg
     5161 acccagagcc gggccgatag gcggcgacgg gttggctttg ccagcgggca cttgcagctt
     5221 gatatagccg acaattttct ttgccacagt aatgctcctg attcgggtca tgacgcaggg
     5281 ttatcagccc tgctgcccag ttaatacgag gcggttgcct caaaaaaatc gtattgcgac
     5341 agtgtcagat caaaagatca gagcttttcg acctgattga attccagttc cactggggta
     5401 tcccggccaa aaatctgcac cgacacacgc agcttgttgc gctcgtagtt gacttcgtcc
     5461 acagcgccat tgaagtcggt gaacgggccg tcgatcacgc gcacgcgctc gcccacttca
     5521 aacagaatct tcggcttggg tttttccacg ccttcttgca tctgctgcat gatggcgtcg
     5581 acttcttttt gcggaatcgg cgccgggcgg ttggcggtgc cgccaacaaa gccagtgact
     5641 ttcggcgtgc tcttcaccaa gtgccagctc tcgtcggtca tttccatctc gaccagcaca
     5701 taaccaggga agaacttgcg ctcggtcagc gagcgcttgc cgtttttcat gtcgaccact
     5761 tcttcaaccg gcaccagaat cttgccgaag cggtcttgca tgttttcgcg ctcgatgcgc
     5821 tctttcaggg ctttttgcac gctcttctcg aagcccgagt aagcgtgaac cacataccaa
     5881 cgcatagcca tcgcttatta gcctcgcttg agcagaatgt cgtagaacaa ccaggacagg
     5941 ccggaatcca ccagccacat gaacagcgcc aacacaaaga cgaacacaaa gaccatcacg
     6001 gtcatttgcg tggcttcttt gcgtgtcggc cacactactt ttttggcttc agcgacagag
     6061 tcttgtgcgt acagcacaaa atccttgccc ggctgagaca gccacaccac gccaccggcc
     6121 gcggcaacgc cagccagcac agccagcaca cgcaacacgc cctgcccctc ggggatcagg
     6181 taaaagccgg ccacaccggc cactacgcac aacagcgcgc caaccagctt gagtttgtcc
     6241 tgggattcca tggaaacaat ttgctcgctt caatacagca aagactaacc ggcgttgtcg
     6301 ccggttagtc ttatttggca ggccaggagg gtctcgaacc cccaaccctc ggttttggag
     6361 accgatactc taccaattga gctactggcc taaaaggggt agagtatacc gcttacttga
     6421 tgatcttggc aaccacgccg gcgccgacgg tgcgaccgcc ttcac
//
LOCUS       DAOPYX010000171         6442 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_14740_length_6442_cov_11.036011, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000171 DAOPYX010000000
VERSION     DAOPYX010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 6442)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6442)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6442
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14740_length_6442_cov_11.036011"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(41..1594)
                     /locus_tag="PKN55_09320"
     CDS             complement(41..1594)
                     /locus_tag="PKN55_09320"
                     /inference="COORDINATES: protein motif:HMM:NF025214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX71486.1"
                     /translation="MTTATLLAQAQAHASTGQHDALLATCQRILDDTHSQDVNALLDV
                     AALLLGSGHVSPAQACLQQAIRLAPQDYRAHVNLANLYREIGEHRASRQLYQQLVHVL
                     PDHPLIRRNALVSLEYDPDASPAVRRTMAQLWGEWAQARVGGRQPRPPAPALNGRPLR
                     VGYVSADFCQHTVGLLVQDVLRAHNPARVSVFAYSAGGVSDWVSRNIAACSQWRDVAA
                     LDDAALAAQVRADGIDVLVDLSGHTAGSRLTAFALRPAPVQASWLGYFATTGLSDIDA
                     LLLDDWHAPPEVAAQCIEPVWRLPRGKFCYQPAPWAPATVAPPPCLIQGHITFGCFNN
                     TSKLNPAVLALWARLLHAIPHARLLLKWRTLIDAGLRQRVANTFAEHGIDPARVSCRG
                     ASFHADLLHEYADIDIALDPFPFTGGLTSCEALWMGVPVLTWPGATPVSRQTWALLAA
                     IGLPELAARDEHDYLRIAQQLASQPEHLNTLRHSLRARMQASPLMDPAGFARQLEDAW
                     LALYGRVAG"
     gene            1831..3831
                     /locus_tag="PKN55_09325"
     CDS             1831..3831
                     /locus_tag="PKN55_09325"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNX71487.1"
                     /translation="MLKPPRRLSVALKISLSVTLLSLLGFALIVGYGAFEHIRQARAQ
                     GMREAELQAELKARWVEERLRAALDTSRELATTYESLKRSQVSDRDMLARLLLNALEQ
                     RTAFQGIWSVWEPNALDGRDASWKDKPMHDGSGRFAPYWRRQGGEVLSTAMAGYSEPS
                     SPYHTLLTRPEPLLSESYRESSGNQSLTLISAATPVMIGGQFLGAVGVTLDLSRIQAD
                     LAAVKLYDTGYLGLLSSQGRWLAHPQPDRVGQSAAELPQAARAALGKGERYRWRSDDG
                     RWMVLAPIQLGETVAPWAVAVSVDADELAADALTVVRNTLITSALGFVAMLALTTWIV
                     RRTLHPLRRMADAMDELSRGQGDLTRRLPVSGHDEISRTAAAFNRFVDCLRDMFAEVK
                     QHSQALDAHLLHLVDNTASVAQTSEQQAQAAQRVNSSVADTSASMARIAAAAQSGRDE
                     ARRTGDLSGELAGGVEATAQAIARAADTVGQLAGALNGLGARSSQIGQIVAVINEIAE
                     QTNLLALNAAIEAARAGEQGRGFAVVADEVRKLAERTASATQEIGGMISAIQREMADA
                     SHTMRDTQARMDDGVSQAEHTAQAISGIQDSMCALLASSQAIAQATQDHAQASETVAS
                     HVRDIHDMTRATDDAIRHTSRASHELQALAAQLDGLVGRFRT"
     assembly_gap    3865..3964
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3994..4872)
                     /locus_tag="PKN55_09330"
     CDS             complement(3994..4872)
                     /locus_tag="PKN55_09330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX71488.1"
                     /translation="MFNSTVLEVAIGMISCYCAVSLIVSSINEGVSSMLQLRGKYLLQ
                     GIQKLLNDPDFNHLAQAVYNNAQFHPAGNGQAVNAKDLNSLPSYASPRQFATALTDTL
                     TTLQTLPGDMGSAIQNIPNPQLRQALSSMYERAGNDLSQFEAEVAYWFDQSMQRLAGA
                     YKRTIQVWTVVFGLVLAMVFNIDTFHLFKVLWLHPTLAANISADQFANAQVAMDQLSV
                     TSLPIGWEKPPFSWGADGPHWNYGLSDFLLMALGWGVTALTTLFGAPFWFDLLQKATH
                     LRGAGNKPASTPNTAS"
     gene            5332..>6442
                     /locus_tag="PKN55_09335"
     CDS             5332..>6442
                     /locus_tag="PKN55_09335"
                     /inference="COORDINATES: protein motif:HMM:NF024140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MCP four helix bundle domain-containing protein"
                     /protein_id="HNX71489.1"
                     /translation="MLKNLTIGVRLTLGFGLLAALLLGVAALAYLRIQDIDSSVQTIS
                     RVNLPKVEQAHAAIDQVNVTARAIRNAMLVKTPDEAQKELDRLTQARKETTELYAKLD
                     KAITSAEGRKMFDTVLETRKITVADQDKVISLFKAGHKDEAADLLVNQVRKSQADYIR
                     ALEVLIRHETELMEQATAHSNTLVDSTARLLLIMAGVALAVAAIGAITVTRSITGPVN
                     QAVEATKRLAEGDLTLNTRSDRRDEMGHLMNALQDMIDKLAHIIGEVNQAGGSLNSAA
                     QQVSATAQSLSQSSSEQAAAVEETTASIEEMTASVNQNSDNARVTNDMALKSADEARE
                     GGAAVRETVDAMKKIADKIGIIDDIAYQTNLLALNA"
BASE COUNT         1223 a         2057 c         2084 g          978 t
ORIGIN      
        1 ggcgtgaata gatactccac ccccgcctca gcctggccgc ctagcccgct acccgcccat
       61 acaaagccag ccaggcgtct tccagttggc gggcaaagcc ggccgggtcc atcagtggcg
      121 aggcttgcat gcgcgcacgc aggctgtggc gcagggtgtt caggtgttcg ggctggctgg
      181 ccagttgctg ggcgatgcgc aggtagtcgt gttcgtcgcg ggcggccagt tcgggcaggc
      241 cgatggcggc cagcagcgcc caggtttgcc ggctgaccgg ggtggcgccg ggccaggtga
      301 gcacgggcac gcccatccac agcgcttcgc agctggtcag cccgccagtg aagggaaaag
      361 gatccagagc gatgtcgatg tcggcgtatt catgcagcag gtcggcatga aacgacgcgc
      421 cccggcagct tacccgcgcc gggtcgatgc cgtgttcggc aaaggtattg gccacgcgct
      481 ggcgcaggcc ggcgtcgatc agcgtgcgcc atttcagcag caggcgggcg tgggggatgg
      541 cgtgcagcag gcgcgcccac agcgccaaga cggccgggtt gagtttggag gtgttgttaa
      601 aacagccaaa ggtgatgtgg ccttggatca gacagggtgg cggggccacc gtggccggcg
      661 cccacggcgc gggctggtag caaaacttgc cgcgcggcag ccgccacacg ggctcgatgc
      721 actgcgccgc cacctcgggt ggggcgtgcc agtcgtccag cagcagcgcg tcgatgtcgc
      781 tcaggccggt ggtggcaaag tagcccagcc aggaggcttg caccggggcc gggcgcaggg
      841 caaaggcggt cagccgcgag ccggcggtgt ggccagacaa atccaccagc acatcaatgc
      901 cgtcggcgcg cacctgggcg gccagcgcgg cgtcgtccag cgcggccaca tcgcgccact
      961 ggctacaggc ggcgatattg cggctgaccc agtccgacac gccgccagcg ctgtaggcaa
     1021 atacgctcac ccgcgccggg ttgtgcgcgc gcaagacgtc ctgcaccaac aggccgacgg
     1081 tgtgctgaca aaaatccgcc gacacatagc ccacccgcag cggccggcca ttcagcgccg
     1141 gggccggcgg gcgcggctgg cggccgccca cccgcgcctg cgcccattca ccccaaagct
     1201 gcgccatggt gcgacgcacc gccgggctgg cgtccgggtc gtattccagg ctgaccaggg
     1261 cattgcggcg gatcagcggg tgatctggca gcacatgcac caattgctga tacaactggc
     1321 gcgaggcgcg atgttcgccg atttcccgat acagattggc caggttgaca tgggcgcgat
     1381 agtcttgcgg cgccaggcgg atggcctgct gcaaacaggc ttgcgccggg ctgacatgcc
     1441 cgctgcccag cagcagcgcg gccacgtcca gcagggcgtt gacgtcctga ctgtgggtgt
     1501 cgtccagaat gcgctgacag gtggccagca gggcatcgtg ctggccggtg ctggcgtggg
     1561 cttgcgcctg ggcaagcaag gtggcggtgg tcatgacgga atcatcgggg caaggggcag
     1621 aaggcccgat gatacccgag gcatcagggg cggcgggttc ggttccccgg acgtggacgt
     1681 ggcctgaggt tcggtttacc ggacgggcaa ggcgctgttt tttagcataa tgttttaaaa
     1741 acagccagtt aaaaaacaca gcatggcgtt ttgccgctgg cgcgctatct gctggagaaa
     1801 caccagtcac ccccggactg gagccccacc atgttaaagc cgccgcgccg cctcagcgtt
     1861 gccctgaaaa tctccctgtc ggtcactttg ctgtcgctgc tgggctttgc cttgatcgtc
     1921 ggctatggcg cgtttgagca tatccgccag gcgcgcgcgc agggcatgcg cgaagcggaa
     1981 ttgcaggccg agctgaaagc ccgctgggtg gaagagcgcc tgcgcgccgc gctggacacc
     2041 tcgcgcgagc tggccaccac ctatgaatca ctcaagcgca gccaggtcag cgaccgcgac
     2101 atgctggcgc ggctgctgct caatgcgctg gagcaacgca ccgcgtttca gggcatctgg
     2161 tcggtgtggg agcccaacgc cctggacggc cgcgacgcca gctggaaaga caaacccatg
     2221 cacgatggct ctggtcgctt tgccccctac tggcggcgtc agggcggcga ggtgctcagc
     2281 accgccatgg ccggctacag cgagccttcc agcccctatc acaccctgct gacacggccc
     2341 gagccgctgc tgagcgaatc ctaccgcgag agctcgggca accagagcct gaccctgatc
     2401 agcgccgcca cgccggtgat gattggcggc cagtttctgg gcgcggtggg ggtgacgctg
     2461 gatttgtcgc gcatccaggc cgacctggcg gcggtgaagc tgtacgacac cggttatctg
     2521 ggcctgctgt ccagccaggg gcgctggctg gcgcatccgc agcctgaccg ggtgggccag
     2581 tcggcggcgg agctgccgca ggcggcgcgc gcggcgctgg gcaagggcga gcgctaccgc
     2641 tggcgcagcg acgacggccg ctggatggtg ctggcgccga ttcaactggg cgaaaccgtg
     2701 gcgccgtggg cggtggcggt gtcggtggat gccgacgagc tggccgccga cgcgctgacc
     2761 gtggtgcgca acaccctgat caccagcgcg ctgggctttg tcgccatgct ggcgctgacc
     2821 acctggattg tgcgccgcac cctccatccg ctgcggcgca tggccgacgc catggacgag
     2881 ctgtcgcgcg gccagggcga cctcacccgc cgcctgccgg tcagcggcca tgacgaaatc
     2941 agccgcaccg ccgccgcgtt caaccgcttt gtcgattgcc tgcgcgatat gttcgccgag
     3001 gtcaaacaac acagccaggc gctggacgcc cacctgctgc acctggtgga caacaccgcc
     3061 agcgtggcgc aaacctcgga gcaacaagcg caggccgcgc agcgggtgaa cagcagcgtg
     3121 gccgacacct cggccagcat ggcgcgcatc gccgccgccg cgcaaagcgg gcgcgacgaa
     3181 gcgcggcgca ctggcgattt gtcgggcgag ctggccggcg gcgtggaagc caccgcccaa
     3241 gccatcgccc gcgccgccga caccgtgggc caactggccg gcgcgctcaa cggcctgggc
     3301 gcgcgctcca gccagatcgg ccagattgtg gcggtgatca acgagattgc cgagcaaacc
     3361 aatctcttgg cgctgaacgc ggccatcgaa gccgcccgcg ccggcgagca aggccggggc
     3421 tttgccgtgg tggccgacga ggtgcgcaaa ctggccgaac gcaccgccag cgccacccag
     3481 gaaatcggcg gcatgatcag cgccatccag cgcgaaatgg ccgacgccag ccacaccatg
     3541 cgcgacaccc aggcgcgcat ggacgacggc gtcagccagg ccgaacacac cgcccaggcc
     3601 atcagcggca ttcaggacag catgtgcgcc ctgctggcct ccagccaggc catcgcccaa
     3661 gccacccagg accacgccca ggccagcgaa accgtggcca gccacgtgcg cgacatccac
     3721 gacatgaccc gcgccaccga cgacgccatc cgccacacct cgcgcgccag ccacgagctg
     3781 caagcgctgg cggcgcagct ggatgggctg gtggggcggt tcaggacgtg agggggaaac
     3841 gctgacgcaa gtagggtgag gcaannnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3961 nnnnggtcgc ggcgcaccgc ccggcgcccg tgctcaactt gcggtattcg gcgtgctggc
     4021 cggtttattg cccgccccgc gcaggtgggt ggctttttgc agcagatcaa accagaacgg
     4081 cgcgccaaac aaggtggtca gcgcggttac gccccagccc agcgccatca gcaagaaatc
     4141 actcaggcca taattccagt gcgggccatc cgcgccccag gagaacggcg gcttctccca
     4201 gcctatcggc aggctggtga cgctcaactg gtccatcgcc acttgcgcgt tggcaaattg
     4261 atcggcgctg atattggccg ccagtgtcgg gtgcaaccac agcaccttga acaaatgaaa
     4321 agtatcaata ttgaacacca tggccagcac caggccaaac accacggtcc acacctgaat
     4381 ggtgcgtttg tacgccccgg ccagtctttg catggattga tcaaaccagt aggccacttc
     4441 tgcctcgaac tgcgacagat cattgccagc ccgctcgtac atgctggaca gcgcctggcg
     4501 cagttgcggg ttggggatgt tctggatggc gctgcccata tcgcccggca gggtttgcaa
     4561 ggtggtcagc gtgtcggtca gcgcggtggc aaactggcgc ggcgaggcat acgagggcag
     4621 gctgttcagg tccttggcat tcacggcctg gccattgccg gccgggtgaa actgggcgtt
     4681 gttgtacacc gcctgcgcca gatggttgaa atccgggtcg ttgagcagtt tttgaatgcc
     4741 ctgcaacaaa tacttgccgc gcaattgcag catggacgac acgccttcgt tgatcgagct
     4801 gacaatcagg ctgacagcgc aatagcagga aatcatgccg atggcgactt ccagaaccgt
     4861 actgttgaac atgggggagc ctttctgagg gggttaagtt tgcgtcactt gccgtcgtcc
     4921 aacccggcag gccgtcgggg gcacaacgcg cgggcggacg gtttgtgaac cgaactacag
     4981 atacaccagc cattccagct cgctgctggc gtctggcgcg cacttcggga ggggtgggcc
     5041 gccagtggat caatcaggcc cagcgcggcg gcctgcatga ataggctcat cggccagtta
     5101 aacgccagag catggcaggc aggagaggcg cagccagcgc cgggcctagc cagtcggatg
     5161 aggcaatatc gccacggcag gcggattttt gccgggcagg gccatctcat gcgcaatatt
     5221 tctgacctgg atcagcacgc aggcatctac atacaaatag attgtttgtt tggtatgctt
     5281 gggccacttg ccaacctagg cgccgtcatc ccccattcaa ggaacagccc catgctcaag
     5341 aatctgacca ttggcgttcg actcaccctc ggctttggcc tattagcggc cttgctgctg
     5401 ggggtggcgg cgctggctta tctgcgtatc caggacattg actcttcggt acaaaccatc
     5461 agccgcgtca atctgcccaa ggtggagcag gcacacgccg cgattgatca ggtgaacgtc
     5521 accgcccgcg ccatccgcaa cgccatgctg gtgaaaacgc cggacgaagc acaaaaagaa
     5581 cttgaccgtc tcacccaggc acgcaaggaa accaccgaac tgtatgccaa gctggacaag
     5641 gcgattacca gcgccgaagg gcgcaaaatg ttcgacaccg tactggaaac gcgcaagatc
     5701 accgtggcag accaagacaa ggtaatcagc ctgttcaagg ccggccacaa agatgaagca
     5761 gctgatttgc tggtcaatca ggtgcgcaag agccaggccg actacatccg cgcactggaa
     5821 gtgctgatcc gccatgaaac cgagctgatg gagcaagcca cagcgcacag caataccctg
     5881 gtggactcca ccgcccgttt gttgttaatc atggctggcg tggcgctggc ggtggccgcc
     5941 atcggcgcca ttaccgtcac tcgcagcatc accggcccgg tcaatcaagc ggtggaagcc
     6001 accaaacggc tggccgaagg cgacctgacg ctgaataccc gcagcgaccg ccgcgacgaa
     6061 atgggccatc tgatgaacgc cctgcaagac atgatcgaca agctcgccca catcatcggc
     6121 gaggtcaatc aagccggtgg ctcgctcaac agcgccgccc agcaggtgtc ggccaccgcg
     6181 caaagcctgt cgcaatcgtc gtccgaacag gccgctgccg tggaagaaac caccgccagc
     6241 atcgaagaaa tgaccgccag cgtgaaccag aacagcgaca acgcccgcgt caccaacgat
     6301 atggcgctga aatccgccga cgaagcgcgc gaaggcggcg ccgccgtgcg cgaaacagtg
     6361 gatgccatga agaaaatcgc cgacaagatc ggcatcatcg acgacatcgc ctaccagacc
     6421 aatctgctgg cgctgaacgc cg
//
LOCUS       DAOPYX010000172         6416 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_14840_length_6416_cov_11.629146, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000172 DAOPYX010000000
VERSION     DAOPYX010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 6416)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6416)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6416
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14840_length_6416_cov_11.629146"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(13..1491)
                     /locus_tag="PKN55_09340"
     CDS             complement(13..1491)
                     /locus_tag="PKN55_09340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008954904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HNX71490.1"
                     /translation="MMPLLDLDSIRAIERAASEHGVDLMARAGLAIAEWIAARHHEDA
                     PILAVAGRGNNGGDALVAGALLLARGYRVDALLPMGEPGSMEAIAALHAFRAAGGNVL
                     SSWDQAHGYALALDGLFGIGLCRPLQDSARRTVERLNDLACPILALDCPSGLDAWTGQ
                     PKPVAVRAAATLSFICPKPGLYTGDGRDYCGDIRIASLGLPEAWTPTWPANSGELNLP
                     EPQQLPMLARRHNSHKGSYGSVAVVGGANGMAGAALLAARAALLMGAGKVFAGFLGEA
                     PPLDGHYPELMLRDAASLLDEAGLTVLAVGPGLGQGASAALALAHALERQLPCVLDAD
                     ALNVLAADAKLAERVRQHPAPCVLTPHPGEAARLLNSRIADSQADRVASARLLAERFQ
                     AVVVLKGAGSLIARPGGHYRLNPTGHAGMAAAGQGDTLTGLIAGLLAQGAPAFDAAAL
                     AVWLAGRGAERLARRGVGPIGLTASETAQAARAEFNRWLRQD"
     gene            complement(1488..2114)
                     /locus_tag="PKN55_09345"
     CDS             complement(1488..2114)
                     /locus_tag="PKN55_09345"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012697502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0106141 - flavin prenyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HNX71491.1"
                     /translation="MSLTALPPATVTLAFTGASGLPYGIRLLEQLLAAGCRVWLLYSP
                     AAQVVAQQEMQLSLPSQAKALQALWREQYRCGEQLSVFGREEWFAPPASGSNPADAMV
                     ICPCSMGALAAIAHGMSDNLIERAADVSIKEKRPLILVPRETPLSVIHLENMLTLARL
                     GVTILPPAPGFYTHPGTVDDMVDFVVARILDQLRIPHTLMARWGEPRT"
     gene            complement(2111..2659)
                     /locus_tag="PKN55_09350"
     CDS             complement(2111..2659)
                     /locus_tag="PKN55_09350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012697501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HNX71492.1"
                     /translation="MPTAPPTLTLHARADDVRVARLAALAVALSLIDAGIPTPLPGLK
                     PGLANIVTLLALTRFGWQVAVSVSLLRIFAASLLLGSLFTPGFFLSLTGGLCSLLGLG
                     LAHRLLPGRWFGAVSLSLIAAFCHIAGQLALARLWLIPSDGILYFVPPFALAACLTGL
                     VNGLACAALLRQPPSPQEPDTP"
     gene            complement(2667..3059)
                     /locus_tag="PKN55_09355"
     CDS             complement(2667..3059)
                     /locus_tag="PKN55_09355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012697500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HNX71493.1"
                     /translation="MARARLSPWLALLRPGDVLMLALAVGLALASFVWLWRDGVAEEV
                     VIRANGQEFARLSLRHDRRIEVPGPLGVTEVQIAAGRARILRDPSPRQYCVRQGWLSR
                     PGQTALCLPNRTSIDIPRRNTPYDSLNF"
     gene            complement(3041..3886)
                     /gene="rapZ"
                     /locus_tag="PKN55_09360"
     CDS             complement(3041..3886)
                     /gene="rapZ"
                     /locus_tag="PKN55_09360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008954908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HNX71494.1"
                     /translation="MQLILISGLSGSGKSVALKILEDSGFYCVDNLPSTLLPQLVENI
                     NAYNYQRVAISVDTRSAPMLSLFPTAVHQLRKLNIDLRLLFLEAKTETLIKRFSETRR
                     RHPLSEEGLTVPECIEKEREMLASIHELSQRIDTSELTANQLRGWIRQLVQADNSRLT
                     LIVQSFGFKHGIPLDADFVFDVRCLPNPYYDPGLRPLTGLDEPVARYLAAEPQVELMF
                     EDIRGMLARWLPEFSRDNRSYLTVAIGCTGGQHRSVYLTERLAAAFASEQVLIRHRQL
                     WREPA"
     gene            complement(3867..4823)
                     /gene="hprK"
                     /locus_tag="PKN55_09365"
     CDS             complement(3867..4823)
                     /gene="hprK"
                     /locus_tag="PKN55_09365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HNX71495.1"
                     /translation="MPSISVRRLYQDNVKKLDLVWVAGSSGTDNRIHADQQRPTLALV
                     GHVNLIHPNSIQVFGLAEIQYLNGLGETDAQRMLDQLFALPISAIIVANDQPLPKGLR
                     AQCEQHNVPLLTSSQPSPYLMDVMRIYLARVLAASTIMHGVFLDVLEVGVLITGESSM
                     GKSELALDLISRGHGLVADDAVELYRIGPETLEGRCPDVLRDFLEVRGLGILNIRAIF
                     GETAVRPKKVLKLIIHLVKATDQSMRELDRLNIQSETQDILDVAIRKVTLPVAAGRNL
                     AVLVEAAVRNYILQLRGIDSTRDFIERQTRFLREQEHAADPD"
     gene            complement(4816..5277)
                     /locus_tag="PKN55_09370"
     CDS             complement(4816..5277)
                     /locus_tag="PKN55_09370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009425064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HNX71496.1"
                     /translation="MSLIAKILSPEHVLLDLDVSSKKRVFEQVGILFENSCGIARSDV
                     FNCLFAREKLGSTGLGQGVAIPHGRAKGLKEASGAYIRLKAPIPYDSPDGKPVQSLFV
                     LLVPDQANDLHLQVLSELAHRFSNRQLRETLLTTTDPAEAHRLLAQWPAHA"
     gene            complement(5527..5856)
                     /gene="raiA"
                     /locus_tag="PKN55_09375"
     CDS             complement(5527..5856)
                     /gene="raiA"
                     /locus_tag="PKN55_09375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011136880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HNX71497.1"
                     /translation="MNLKITGIHLEVTPSLREYIEAKLERITRHVDNVTSVAVTLSVE
                     KLEQKAEVNVHLKGKDIHVEATSTDMYAALDALADKLDRQVLKHKEKITDHHSGERAP
                     TPATDEA"
     gene            complement(6068..>6416)
                     /locus_tag="PKN55_09380"
     CDS             complement(6068..>6416)
                     /locus_tag="PKN55_09380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008954912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HNX71498.1"
                     /translation="MRPLVLRDIADELGLHESTISRVTTQKFLLCPRGIFELKYFFGS
                     ALETDSGGECSATAIKARLRQLIANEDPKKPLSDSALADMLGNEGIQVARRTVAKYRE
                     AMQIPPVNLRKSI"
BASE COUNT         1127 a         2058 c         2164 g         1067 t
ORIGIN      
        1 aagccaaatc gctcagtcct gacgcagcca gcggttgaat tccgcccgcg ccgcctgcgc
       61 ggtttcgctg gcggtcaggc cgatggggcc aacgccgcgc cgcgccagcc gttccgcgcc
      121 gcgtccggcc agccacaccg ccagcgcggc ggcgtcgaag gcgggcgcgc cttgcgccag
      181 caggccggca atcaggccgg tgagggtgtc gccctggccg gcggcggcca tgccggcgtg
      241 gccggtgggg ttcagccggt agtggccgcc ggggcgggca atcaggctgc cggcgccttt
      301 gagcaccacc accgcctgaa agcgttcggc cagcaggcgg gcgctggcca cccggtcggc
      361 ctggctgtcg gcgatgcggc tgttcaatag ccgtgcggct tcgccagggt gcggggtgag
      421 cacgcacggc gccgggtgct ggcgcacccg ctcggccagc ttggcgtcgg ccgccagcac
      481 attcagcgcg tcggcgtcca gcacgcaggg cagttgccgc tccagcgcgt gcgccagcgc
      541 caatgccgcg ctggcgccct ggcccagacc ggggccgacc gccagcacgg tcagccccgc
      601 ttcgtccagc aggctggcgg cgtcgcgcag catcagctcc gggtagtgcc catccagcgg
      661 cggcgcttca cccagaaaac cggcaaacac cttgccggcg cccatcaaca gcgccgcccg
      721 cgccgccagc agcgccgcgc cggccatgcc attggccccg cccaccaccg ccacgctgcc
      781 gtaactgcct ttgtggctgt tgtggcggcg cgccagcatc ggcagttgtt gcggttcggg
      841 caggttcagc tcgccgctat tggccggcca ggtgggcgtc cacgcctcgg gcaggcccag
      901 gctggcgatg cggatgtcgc cgcagtagtc gcggccgtcg ccggtgtaca agcccggttt
      961 ggggcagata aagctcagcg tggcggcggc gcgcaccgcc accggcttgg gctggccggt
     1021 ccaggcgtcc aggccggacg ggcagtccag cgccagaatc gggcaggcca ggtcgttcag
     1081 ccgctcgacc gtgcgccggg cggagtcctg caagggccgg cacaggccaa tgccaaacaa
     1141 cccatccagc gccagcgcgt agccatgcgc ctgatcccag ctggacagca cgttgccgcc
     1201 cgcggcgcga aaggcgtgca gcgcggcaat cgcctccatg ctgcccggct cgcccatcgg
     1261 cagcagcgcg tccacccggt agccgcgcgc cagcagcagc gcgccggcca ccaaggcgtc
     1321 gccgccattg ttgccgcgcc cggccaccgc caggatcggc gcgtcttcgt ggtggcgggc
     1381 ggcaatccac tcggcaatcg ccagcccggc gcgcgccatc aggtctacgc cgtgttcact
     1441 tgccgcgcgc tcgatggcgc gaatgctgtc cagatcaagc aagggcatca tgtgcgtggt
     1501 tctccccagc gcgccatcag cgtgtgcgga atgcgcaatt ggtccaggat gcgggcgacg
     1561 acaaaatcca ccatgtcatc caccgtgccg gggtgggtgt aaaaccctgg cgccggcggc
     1621 aggatggtga cgcccagccg ggccagcgtc agcatgtttt ccaggtgaat caccgacaag
     1681 ggggtttcgc gcggcaccag gatcaacggg cgtttttcct tgatgctgac atcggcggcg
     1741 cgttcgatca ggttgtcgct catgccgtgg gcgatggcgg ccagcgcgcc catcgagcac
     1801 gggcagatca ccatggcgtc ggccgggttg gagcccgatg ccggcggggc aaaccattct
     1861 tcgcggccaa acaccgataa ttgctcgccg cagcggtatt gctcgcgcca cagcgcttgc
     1921 agcgccttgg cctggctggg caggctcagt tgcatttctt gttgcgccac cacttgcgcg
     1981 gcgggcgaat acagcagcca cacccggcag ccggcggcga gcaattgctc cagcaggcgg
     2041 atgccgtagg gcaagccaga agcgccggta aaggccaggg tgacggtggc ggggggcaaa
     2101 gcagtcaggc tcatggggtg tcgggctctt ggggggaagg cggctggcgc aataaggcgg
     2161 cgcaagccag gccgttgacc aggccggtca ggcaggcggc cagggcaaac ggcggcacaa
     2221 aatacaaaat gccgtcagac ggaatcaacc acagccgcgc cagcgccagc tggccggcaa
     2281 tatggcaaaa cgcggcgatc aggctcaggc tgaccgcgcc aaaccagcgc cccggcagca
     2341 gccggtgcgc caggcccagg cccagcaggc tgcacaaccc gccagtcagg ctgagaaaaa
     2401 accccggcgt aaacaggctg cccaacagca ggctggcggc aaagatgcgc agcaggctga
     2461 cgctgaccgc cacctgccag ccaaagcggg tcagcgccag cagggtgaca atattggcca
     2521 ggcccggttt caggccgggc aagggggtgg gaatgccggc gtcgatcagc gacagcgcca
     2581 ccgccagcgc agccaggcgc gccacgcgca catcgtcggc gcgggcatgc agggtgaggg
     2641 tgggcggggc ggtgggcatg gcggcgttaa aagttcaggc tgtcgtaagg ggtattgcgg
     2701 cgcgggatgt cgatcgaggt gcggttgggc aggcacagcg cggtttgtcc gggtcggctc
     2761 agccaaccct ggcgcacgca atactggcgt gggctggggt cgcgcaggat gcgcgcccgc
     2821 ccggcggcga tttgcacttc ggtgacgccc agcgggccgg gcacttcgat gcggcggtcg
     2881 tggcgcaggc tcagccgggc aaactcctgg ccgttggcgc ggatcaccac ttcttcggcc
     2941 acaccgtcgc gccacaacca gacaaagctg gccagcgcca ggcccacggc cagcgccagc
     3001 atcagcacat cgccagggcg cagcagcgcc agccaggggc ttaagcgggc tcgcgccaca
     3061 attgccggtg acggatcagc acctgctcgc tggcaaacgc cgccgccagc cgttcggtca
     3121 ggtacaccga gcgatgctgg ccgccagtgc agccaatggc caccgtcagg taactgcggt
     3181 tgtcgcggga gaattccggc agccaacggg ccagcatgcc gcgaatgtcc tcgaacatca
     3241 gctccacctg cggctcggcg gccagatagc gcgccaccgg ctcgtccagc ccggtcagcg
     3301 gccgtaaacc ggggtcgtaa tacgggttgg gcagacagcg cacgtcaaac acaaagtcgg
     3361 cgtccagcgg aatgccgtgc ttgaagccaa atgactgcac aatcagcgtc agccggctgt
     3421 tgtcggcttg caccagctgg cgaatccagc cgcgcaactg gttggcggtg agttcactgg
     3481 tgtcgatgcg ctggctgagc tcatgaatgg acgccagcat ctcgcgttct ttttcgatgc
     3541 actcgggcac cgtcaggcct tcttccgaca gcggatggcg gcggcgcgtt tccgaaaagc
     3601 gcttgatcag ggtttcggtt ttggcttcga gaaacagcag acgcaggtcg atattcagct
     3661 tgcgcagctg gtgcacagcg gtgggaaaca gcgacagcat cggcgcgctg cgggtgtcca
     3721 cgctgatggc cacgcgctgg taattgtagg cgttgatgtt ttccaccagc tgcggcagca
     3781 gggtggaggg cagattgtcc acgcagtaaa aaccggagtc ttccagaatt ttcagcgcca
     3841 ccgacttgcc cgaaccggac agcccgctaa tcaggatcag ctgcatgttc ttgctcccgg
     3901 agaaaacggg tttgtcgttc gatgaaatcg cgcgtgctgt cgatgccgcg caattgcaga
     3961 atatagttgc gcaccgccgc ctcgaccagc accgccagat tgcgtccggc ggccaccggc
     4021 agggtgactt tgcggatggc gacgtcgagg atgtcctggg tttcgctctg gatgttcagg
     4081 cggtccagct cgcgcatgga ttgatcggtg gccttgacca gatggatgat cagcttgagc
     4141 acttttttcg ggcgcacggc ggtttcgcca aaaatcgccc ggatgttcaa aatgcccagc
     4201 ccgcgcactt ccagaaaatc gcgcagcacg tccggacagc ggccttccag cgtttccggg
     4261 ccaatgcgat acagctcgac ggcgtcgtcc gccaccaggc catggccgcg cgagatcaaa
     4321 tccagcgcca gctcgctttt gcccatcgac gattcgccag tgatcagcac gcccacttcc
     4381 agcacgtcca gaaacacgcc atgcatgatg gtggaggcag ccagcactcg cgccagataa
     4441 atgcgcatca cgtccatcag atagggcgag ggctgcgacg aggtcagcag cggcacattg
     4501 tgctgctcgc actgcgcgcg caggcccttg ggcagaggct ggtcgttggc gacgatgatg
     4561 gcggaaatcg gcagggcgaa cagctgatcc agcatgcgct gggcatcggt ttcgcccagg
     4621 ccgttcaggt actggatttc cgccaggcca aacacttgaa tgctgttggg atgaatcagg
     4681 ttgacgtggc cgaccagcgc cagcgtgggg cgctgctggt cggcgtgaat gcggttatcg
     4741 gtgccggagc tgccggccac ccataccaaa tccagttttt tgacattgtc ctgatacagg
     4801 cgacgaacac tgatgctagg catgggcagg ccattgggcc agcaggcggt gcgcctcggc
     4861 cgggtcggtg gtggtcagca gcgtttcacg cagctgccgg ttggaaaaac ggtgcgccag
     4921 ctcggacagc acctgcaaat gcaggtcgtt ggcctgatcc ggcaccagca agacaaacag
     4981 gctttgcacc ggcttgccgt cgggcgaatc ataggggatg ggcgctttca ggcggatata
     5041 ggcgccgctg gcttctttca gccccttggc gcggccatgc ggaatcgcca ccccttgccc
     5101 cagcccggtg gagccgagtt tttctcgggc gaacaggcaa ttgaacacat cgctgcgggc
     5161 gatgccgcag ctgttttcaa acaggatgcc gacctgctcg aacacccgct ttttgctgga
     5221 gacgtccaga tcaagcagca cgtgctcggg agacaggatt ttggcaatca gactcattgc
     5281 gtgacccacg ctttcacgga atcgcatcat tctacgccgg tttgaccggg ctggcagccg
     5341 acgcgcaagg ctggccggtc ggcgctgaca caggccagca gcgccgacag catgttttgc
     5401 ccatggcgca gcagcgcgcg gcgtcatggg caaaacagac tgggggaaat caccgccggc
     5461 gggcatctgg gagatggccg ccggcatggg aggcgcggcg cgcagcaggg cgcgccggca
     5521 aaagaatcag gcttcgtcgg tcgccggggt cggggcgcgc tcgcccgagt ggtgatcggt
     5581 gatcttttcc ttgtgcttga gcacctggcg atccagcttg tcggccagcg cgtccagcgc
     5641 ggcgtacata tcggtgctgg tggcctcgac gtgaatgtcc ttgcccttga ggtgcacatt
     5701 cacctcggct ttttgttcca gtttttccac cgacagggtg accgccaccg aggtgacgtt
     5761 gtcgacgtga cgggtgatgc gctcgagctt ggcctcgatg tattcgcgca gcgacggggt
     5821 gacttcgaga tggatgccgg tgattttcag gttcataacc ttactccttc ctggtggtgc
     5881 gtcccgccac tgggcggaac tggttgaagc ggcgcaacaa acgcctgtct gttgcgaaag
     5941 ccggcacatc aggacatttc atgccctggt aatgtttaca tcggccggcg cgcggccggc
     6001 ttgagtggcg ttcaggcaaa ccggcgtcaa aggtggactt cggtttgcgc gtgatgttcc
     6061 ctatgcctca gatcgacttg cgcagattga ccggcggaat ctgcatggcc tcgcgatact
     6121 tggccaccgt gcgccgggcc acctgaatgc cctcattgcc cagcatatca gccagcgccg
     6181 aatcggacag cggttttttc gggtcttcgt tggcgatcag ctggcgcagc cgcgccttga
     6241 tggcggtggc cgagcactcg ccgcccgagt cggtttccag cgcgctgccg aagaaatatt
     6301 tcaattcaaa gataccacgc ggacataaaa ggaatttttg cgtggtcacc cgcgagatgg
     6361 tggattcgtg caggcccagc tcgtcggcaa tgtcgcgcag caccagcggg cgcatg
//
LOCUS       DAOPYX010000173         6367 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_14999_length_6367_cov_9.016477, whole genome shotgun sequence.
ACCESSION   DAOPYX010000173 DAOPYX010000000
VERSION     DAOPYX010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 6367)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6367)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6367
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14999_length_6367_cov_9.016477"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1100)
                     /locus_tag="PKN55_09385"
     CDS             complement(<1..1100)
                     /locus_tag="PKN55_09385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018748044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNX71499.1"
                     /translation="MAAKSMLERLLPDTLEQDEAIQARTVIMVALLAGGLAPLFALSY
                     YKLHHYPMAHGIVLGGVGMVLAIALLKLTGRVWLAAEYVTTVMFGMVSWMVYVNNGIM
                     STSIIWYACIPVAAVFVSGRRAGYVWSVLTFAAIGLFLLAHDQPGWLPVSPIPHEALP
                     SLQAKSLIGLTIVVLALVLAFEGAKSRGFTKLEQAREEAERSHASLARMLEQVTRSIR
                     AASRESKEIADSAHMMATTMNQQTRRTHDMVSAVQEMDAMTRKNADASSQAANVSREA
                     ESQAGDGGEVMDSAMRRLTEASEVIGRAAAQMEELGRRSSEVSGIAQMIAEIADQTNL
                     LALNAAIEAARAGEAGRGFAVVADEVRKLAERT"
     gene            1444..1533
                     /locus_tag="PKN55_09390"
     tRNA            1444..1533
                     /locus_tag="PKN55_09390"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1478..1480,aa:Ser,seq:cga)
     gene            1667..2512
                     /gene="rlmJ"
                     /locus_tag="PKN55_09395"
     CDS             1667..2512
                     /gene="rlmJ"
                     /locus_tag="PKN55_09395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase
                     RlmJ"
                     /protein_id="HNX71500.1"
                     /translation="MLSYRHAFHAGNHADVLKHWVEILLLDHLGRKDKPYWYIDTHAG
                     AGAYALDSAYASKNAEYNTGIGRLWTRDDLPPSLADYVALVKTFNPDGQLRAYPGSPM
                     VAQRRLRASDRMRLFELHSTDHGLLAENFAHAQRQTRIEHADGFDGVKAILPPPPRRA
                     LMLIDPPYEDKRDYARVLTCLKESFKRFATGCYAVWYPQLQRLESRDLPEKLKLLGEM
                     DWLHVSLSVQTPSADGFGMHGSGMFVLNPPWTLPEVLKAELPYLVKALGQDAGARYTL
                     DWRIR"
     gene            complement(2613..2738)
                     /locus_tag="PKN55_09400"
                     /pseudo
     CDS             complement(2613..2738)
                     /locus_tag="PKN55_09400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003742505.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
     gene            complement(2735..5068)
                     /gene="hypF"
                     /locus_tag="PKN55_09405"
     CDS             complement(2735..5068)
                     /gene="hypF"
                     /locus_tag="PKN55_09405"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018749474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HNX71501.1"
                     /translation="MAWLPACPALPDPARLRLRLLGGVQGVGLRPAIWRLSQRWPLAG
                     WVRNDGDGACIELEGARADLHAWLAALPAALPPLAQVDHAELSVQPCQGETGFAVIDS
                     QPGAGGGARLPPDLAPCPACLAELFDPSNRRWRHPFINCTDCGPRFSVSRQLPYDRAH
                     TSLAGFALCADCASEYRQPADRRFHAEPIACPHCGPRLSLCDLNGSPVAGDALSHTVA
                     LLRAGQIVAIKGVGGFHLVCDADQPQAVAMLRARKRRPAKPLAVMALNADSLADVADI
                     APLAARWLNDPSRPVVLSPARAGLDALWPGIAPGVGRVGAVLPMSPLHYLLFHEAAGR
                     PSGVDWLTQPHPLRLVFTSANLSGAPLITDNAQALRDLAGLADFILLHDREIVARCDD
                     SVLAVRGARQVWVRRARGRTPTPLAAAPGEPVLALGAQLKNTLTVASGNGWHTSQHLG
                     DLDHPDSWDVLEDAVRHWLTLTGAQPTRIAHDRHPDALSSRLAAELALRFDAELIPVQ
                     HHHAHLAAVLAEHHWPADQPVLGLALDGFGLGDDGGAWGGELLRVRGADCQRLGRLRP
                     LALPGGDIAARQPWRMAASALAALGHREADIAARLATHPGAAAVAAMLARGVNCPPTS
                     SLGRAFDAVAALAGVCAEQSYESEAAQKLEAHAQGPWPAPDPALWRLADDASELDLRP
                     LLACISTLNDVHASAALWHANLAAALAHWAHTASQRQGVRHIALAGGVCLNLTLLDAL
                     CAQLTAAGLTPLLAIELPPNDGGVSLGQAWVARHLGR"
     gene            complement(5068..6000)
                     /gene="hypB"
                     /locus_tag="PKN55_09410"
     CDS             complement(5068..6000)
                     /gene="hypB"
                     /locus_tag="PKN55_09410"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF007775.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HNX71502.1"
                     /translation="MCTVCGCGPDQVHIDGQAPGHFNYRALGRKPAATAVAAAPSPVS
                     ASEGALDYGAGPARAHAPGLSQARMVKIEQDILSKNDQYAQRNRDWLEERSVLALNLV
                     SSPGSGKTTLLAATVARLLPQMPVAVIEGDQQTSHDAERIRAAGAPAVQINTGKGCHL
                     DGHMVGEALQRLPLDEQGVLFIENVGNLVCPAAFDLGETAKVAILSVTEGEDKPLKYP
                     DMFAAARLMILNKIDLLPHLDFDVAACIDYARRVNPAIEVLQLSARTGEGMDAWLAWL
                     AARRGEVIQAGQHTVSELKARIAALEAQLAAQGL"
BASE COUNT         1098 a         2168 c         2052 g         1049 t
ORIGIN      
        1 gtgcgttcgg ccagcttgcg cacttcgtcg gccaccacgg caaagccgcg cccggcttcg
       61 cccgcgcggg cggcctcaat cgccgcgttc aaggccagga ggttggtctg gtcggcaatc
      121 tcggcaatca tctgggcaat gccgctgact tcgctgctgc gccggccaag ctcttccatt
      181 tgcgctgccg cgcggccaat cacctcgctg gcctcggtca gccggcgcat ggcgctgtcc
      241 atcacctcgc cgccgtcgcc ggcctggctt tccgcttcgc gcgaaacatt ggctgcctgg
      301 ctggaggcgt cggcgttttt gcgcgtcatg gcgtccatct cctgcacggc gctgaccata
      361 tcgtgggtgc ggcgggtttg ctggttcatg gtggtggcca tcatgtgcgc ggaatcggcg
      421 atttccttgc tttcgcggct ggcggcgcgg attgagcggg tgacttgctc cagcatgcgc
      481 gccaggctgg cgtgcgagcg ctcggcttct tcgcgggctt gctccagctt ggtgaagccc
      541 cggctcttgg cgccttcaaa agccagcacc agcgccagca cgacaatggt caggccaatc
      601 agtgatttgg cttgcaggct aggcaaggcc tcgtgcggaa tcggcgacac cggcagccag
      661 ccgggttgat cgtgcgccag cagaaataaa ccaatggcgg caaaggtgag cacgctccac
      721 acatagccgg cgcgacggcc ggagacaaac accgccgcca ccgggatgca ggcgtaccag
      781 atgatcgagg tggacatgat gccgttgttg acatacacca tccagctgac catgccaaac
      841 atcaccgtgg tgacgtattc cgccgccagc cacacccggc cggtgagttt gagcagggcg
      901 atggccagca ccatgcccac gccgcccagc acaatgccgt gcgccatcgg gtagtggtgc
      961 agcttgtaat aagacaaggc aaacagcggc gccaggccgc cggccagcag cgccaccatg
     1021 atcacggtgc gcgcctggat ggcttcgtct tgttcgagtg tgtccgggag caaacgctcc
     1081 agcatggatt tggccgccat gtttcccctc tttcctcgcg gtggtgcagt cgcgtgcttg
     1141 tgcgcgacgg tctggtgtgt ggctgcacag cgcatgctgt tgagaatttt ttgcgttgca
     1201 atacaagtca ctgcacgcaa tcatgcgtct gtttggcgag gggattatgc cccttttttt
     1261 cagcaaatca aacgggtgtt tgagttattt tgttgttgca ctgcgatgga ttggccaaat
     1321 tcgccacact gtggcgcgtg tcgccagcac cacaagcagc gggccaaatc ggcctgagtc
     1381 aatttttttt gcaagaaccg cttgccaaaa ctgaaaaccg tgtctaatat acgcatctct
     1441 tttggagaga tgccggagcg gctaaacggc cctgactcga aatcagtggg ggacgcaagt
     1501 cccactaggg ttcgaatccc tatctctccg ccagcatcac cgcaaaagcc cttgattttt
     1561 caagggcttt tgctttttgt gcgccacgtc ccgcttaggc tggccttcgc acctggcgcg
     1621 ctgcttatcg aaatcagtcg ccgcccggcg gccaaaggac tcgcccatgc tcagctaccg
     1681 tcacgccttc catgccggca accacgccga tgtgctcaag cactgggtgg aaatcctgct
     1741 gctggatcac ctgggccgca aagacaagcc ttattggtat atcgacaccc acgccggcgc
     1801 tggcgcctat gcgctggaca gcgcctacgc cagcaaaaac gccgaataca atactggcat
     1861 cggccggctg tggacgcgcg acgacctgcc gccctcgctg gccgactatg tggcgctggt
     1921 gaaaaccttc aatcccgatg gccagctgcg cgcctacccc ggttcgccaa tggtggcgca
     1981 gcgccggctg cgcgccagcg accgcatgcg cctgttcgag ctgcacagca ccgaccacgg
     2041 cctgctggcg gaaaacttcg cccacgccca gcggcaaaca cgcattgagc acgccgacgg
     2101 ctttgatggc gtgaaagcca tcctgccgcc gccaccgcgc cgcgcgctga tgctgatcga
     2161 ccccccctat gaagacaaac gcgactacgc ccgcgtgctg acctgcctga aagaatcgtt
     2221 caagcgcttt gccaccggct gctatgcggt gtggtatccg caattgcagc ggctggaatc
     2281 gcgcgacctg ccggaaaaac tcaagctctt gggcgagatg gattggctgc acgtcagcct
     2341 gtcggtgcaa accccgtcgg ccgacggctt tggcatgcac ggcagcggca tgtttgtgct
     2401 gaatccgccg tggacgctgc cggaagtgtt aaaagccgag ctgccgtatc tggtcaaggc
     2461 gctggggcag gatgccggcg cgcgctacac gctggattgg cggattcgct aacaatcgcc
     2521 aagaaaactc agcgctgcgc gcctggcatg cagccgcaga caataccagt agtacggcaa
     2581 gaccgggtaa cctgcatggc cgattggcgg gcaaagcctt gcgccgtgcc ttgccggtgt
     2641 agccagatgc gcggcgccaa gttggacgtc acgccgatgt acagcgtgcc gttgccttgg
     2701 ctggccagga tagacaccgc tggcgcgcgc ctggttatcg gccgaggtgt cgcgccaccc
     2761 acgcttgccc caggctgacg ccgccgtcgt tgggcggcag ttcaatggcc agcagcgggg
     2821 tgaggccggc ggcggtgagc tgggcgcaca gggcgtccag cagggtgagg ttcaggcaga
     2881 cgccgccggc cagcgcgatg tggcgcacgc cttggcgctg gctggcggtg tgcgcccagt
     2941 gggccagcgc cgccgccagg ttggcgtgcc acagcgcggc gctggcgtgg acgtcgttga
     3001 gggtgctgat gcacgccagc agcgggcgca ggtccagctc ggatgcgtca tcggccagcc
     3061 gccacagcgc cgggtcgggc gcgggccagg ggccttgggc gtgggcttcc agtttttgcg
     3121 cggcttcgct ttcgtagctt tgttcggcgc agacgccggc cagcgcggcc acggcgtcga
     3181 aggcgcggcc caggctgctg gtgggcgggc agttgacgcc gcgcgccagc atggcggcca
     3241 ccgccgccgc gccggggtgc gtcgccagcc gggcggcgat gtcggcttcg cggtggccca
     3301 gcgccgccag cgcgctggcg gccatgcgcc agggttggcg ggcggcgatg tcgccgccgg
     3361 gcaaggccag cgggcgcagc cggcccaggc gctggcagtc ggcgccgcgc acgcgcagca
     3421 gctcgccgcc ccaggcgccg ccatcgtcgc ccaggccaaa accatccagc gccaggccca
     3481 gcactggctg gtcggccggc cagtggtgtt cggccagcac ggcggccaga tgggcgtgat
     3541 ggtgttgcac cgggatcagc tcggcgtcaa agcgcagcgc caattcagcg gccagccgcg
     3601 acgacagcgc gtccgggtgg cggtcgtggg cgatgcgcgt gggttgcgcg ccggtcagcg
     3661 tcagccagtg acgcacggcg tcttccagca cgtcccagct gtccgggtga tccaggtcgc
     3721 ccagatgctg cgaggtgtgc cagccgttgc cgctggccac ggtgagggtg tttttcagct
     3781 gcgcgcccaa ggccagcacc ggctcgccag gcgcggcggc cagcggcgtc ggcgtgcggc
     3841 cgcgcgcgcg gcgcacccac acctgacgcg cgccgcgcac cgccagcacg ctgtcgtcac
     3901 aacgggcgac aatctcgcgg tcgtgcagca aaataaaatc cgccagccca gccaggtcgc
     3961 gcagcgcctg ggcgttgtcg gtaatcaacg gcgcgccgct gaggttggcg ctggtgaaca
     4021 ccagccgcag cgggtgcggc tgcgtcagcc agtccacccc gctggggcgg ccggcggctt
     4081 cgtgaaacag caggtaatgc aatggcgaca tcggcagcac tgcgcccacc cggccgacgc
     4141 cgggggcaat gcccggccag agggcgtcca gcccggcgcg cgccgggctg agcaccaccg
     4201 ggcggctggg gtcgttcagc cagcgcgccg ccagcggggc gatgtcggcc acgtcggcca
     4261 atgaatcagc gttgagcgcc atcaccgcca gcggcttggc cggccggcgc ttgcgcgcgc
     4321 gcagcatggc caccgcctgc ggctggtcgg cgtcgcacac cagatgaaaa ccgcccacgc
     4381 ccttgatggc gacaatctgc ccggcgcgca gcaaggccac ggtgtgcgac agcgcatcgc
     4441 cggccaccgg gctgccgttc aggtcgcaca ggctgagccg ggggccgcag tgcgggcagg
     4501 caattggttc ggcatgaaaa cgccggtcag ccggctggcg gtattcgctg gcgcagtcgg
     4561 cgcacagggc aaagccggcc aggctggtgt gggcgcggtc atacggcagc tggcggctga
     4621 cgctaaagcg cgggccgcag tcggtgcagt tgataaacgg atgccgccag cggcggttgc
     4681 tggggtcgaa cagctcggcc aggcaggccg ggcacggggc caaatccggc ggcaggcgcg
     4741 cgccgccgcc cgcgccgggc tggctgtcga tcacggcaaa ccccgtttct ccctgacacg
     4801 gctgcacgct gagttcagca tggtccacct gcgccagcgg cggcagcgca gcaggcagcg
     4861 ccgccagcca ggcgtgcaga tcggcgcgcg cgccttccag ctcgatgcat gcgccgtcgc
     4921 cgtcattgcg cacccaaccg gccagcggcc agcgctggct cagccgccag atcgccgggc
     4981 gcaaacccac gccctgcact ccacccagca agcgcagccg cagccgcgcc ggatcgggca
     5041 gcgccgggca ggccggcagc caggccatca cagcccctgc gcggccagtt gcgcttccag
     5101 cgcggcaatg cgcgccttca gctcggacac cgtgtgctgg cccgcctgaa tcacctcgcc
     5161 gcgccgcgcc gccagccacg ccagccaggc gtccatgccc tcgcccgtgc gcgccgacaa
     5221 ttgcagcact tcgatcgccg gattcacccg gcgggcgtaa tcaatgcagg cggccacgtc
     5281 aaaatccaga tgcggcagca ggtcgatctt gttcaaaatc atcaaccggg cggcggcaaa
     5341 catatccggg tatttcaacg gcttatcttc gccctcagtc accgacaaaa tcgccacctt
     5401 ggcggtttcc cccagatcaa acgccgccgg gcacaccaga ttgccgacgt tttcaataaa
     5461 caacacgccc tgctcgtcca gcggcaaacg ttgcagcgcc tcgcccacca tatgcccatc
     5521 cagatggcag cccttgccgg tgttgatctg caccgccggc gcacccgccg cgcgaatgcg
     5581 ctcggcgtcg tggctggttt gctgatcgcc ttcaatcacc gccaccggca tctgcggcaa
     5641 caaacgcgcc accgtcgccg ccagcagcgt ggtcttgccc gaaccggggc tggacaccag
     5701 attcagcgcc aacacactgc gctcctccag ccaatcgcgg ttgcgctggg cgtattggtc
     5761 gtttttggac aggatatcct gctcaatctt caccatccgc gcctgactca agcccggcgc
     5821 atgcgcccgc gccggccccg cgccataatc caacgcccct tcactggccg acacaggcga
     5881 cggcgcagca gcaaccgccg tagccgccgg cttgcggccc aaagcgcgat aattgaaatg
     5941 accgggcgcc tgcccgtcga tatggacctg atccgggcca cagccacata ccgtacacat
     6001 aaggttctcc ttgtcggccc tgctccggcc tgcctgttgc tatccgcgcc gaccacgacg
     6061 cgcatcctgc ttgcccactg atcgcggcaa gtattgttat tttttatgga ctgcttttaa
     6121 aaccaaaaac caaacaaaat caaaaacctg gattcccgcc tgcgcgggaa tgacggggag
     6181 acgcaaggga gacgcagcgg taaggcatta aacaaccgca agacatttaa attgccaagc
     6241 attaaataaa acaccttcaa caccaagcaa gccaactatt caagcaccgc ccgtcgcgcc
     6301 tagccgattt accccccgtg tggcgcgctg agcatcgcag ccggacaggg aggtgtccgc
     6361 cacggct
//
LOCUS       DAOPYX010000174         6334 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_15121_length_6334_cov_9.015767, whole genome shotgun sequence.
ACCESSION   DAOPYX010000174 DAOPYX010000000
VERSION     DAOPYX010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 6334)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6334)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6334
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15121_length_6334_cov_9.015767"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(34..843)
                     /locus_tag="PKN55_09415"
     CDS             complement(34..843)
                     /locus_tag="PKN55_09415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014088018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HNX71503.1"
                     /translation="MRRHFLKLAVALAVSGALSTSALAADPAKPELVFGTTVGDFGDM
                     VKLSIKPILEKQGYKVKLVEFTDYVRPNLALAEGSIDVNIFQHKPYLDNFAKEHKLAL
                     TPVVQVPTGPLGLYSGKQKALAAVKAGSTVAVPNDPTNQARALVMLADLGWITLKPGI
                     DPLTASERDVEKNLKQIKIVPLEAAQLPRARGDVDFAVVNGNYAVSSGMKFTEALALE
                     KSYAYVNWVVVRTADVNKPFVKDITAAYNSAEFKAWAKTRFAGYKLPQSWK"
     gene            complement(949..1614)
                     /locus_tag="PKN55_09420"
     CDS             complement(949..1614)
                     /locus_tag="PKN55_09420"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019922474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HNX71504.1"
                     /translation="MDFSNLLSMAPEIWQASLETALMLGIGLTVAIVLGGPLGIVLFL
                     TQPGQLWAHRALNGVLGWLVNLVRSFPFIILMVSLVPLTRLLVGSTIGPVAAAVPLSF
                     AAIPYFARLVEQTLREVPRGVLEAAEAMGASPWQIIRKVLINEARAGLVSSLTILTIS
                     FLSYSAVAGVVGGGGIGDLAIRYGYYRFQTDVMLAMVALLVVLVQLIQWLGARLAKAL
                     DKR"
     gene            complement(1616..2353)
                     /locus_tag="PKN55_09425"
     CDS             complement(1616..2353)
                     /locus_tag="PKN55_09425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNX71505.1"
                     /translation="MIALKQVSKRFVRPDGSVFTALSPSDLEIQAGEIFGLIGPSGAG
                     KSTLLRLINLLERPDGGQVVVDGQTLTALPAAGLRRARQNIGMIFQQFNLMANRTVAD
                     NVAFPLEIAGWPAAVRAARVAECLALVGLADRAGDYPAQLSGGQKQRVGIARALAPRP
                     HVLLADEPTSALDPATTRGILDCLADINQRLGVTVVIVTHEMAVVRAICHRAAVVTQG
                     TIAEIFPVIDGVAQANSAAGLALLEEA"
     gene            2719..3702
                     /gene="tauA"
                     /locus_tag="PKN55_09430"
     CDS             2719..3702
                     /gene="tauA"
                     /locus_tag="PKN55_09430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018150105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA];
                     GO_function: GO:0030977 - taurine binding [Evidence IEA];
                     GO_process: GO:0015734 - taurine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HNX71506.1"
                     /translation="MSHPALKSLYVALSLLAGTASAADPVTIAYQTGIDPSKVAQADG
                     AYEAATGSKINWRKFESGAEVIAAVASGDVPIGNIGSSPLAAAASRGLPIQTFLVTAE
                     IGDSEALVVRNGSNIAAPKDLVGKKVAVPFVSTTHYSLLAALKHWGVDASKVNIVNLR
                     PSEIAAAWQRGDIDAAYVWEPALGTAKASGKVLVSSAQVGKWGAPTYDLWIVRKDFAE
                     KNPEFLTQFVKVTGAAYAKYNADPKGYAANNANVDKIARLTGAKPQDVALLLAGNTYP
                     SLKRQAELLDKPFAEAVGKTAEFLRAQGKVEQVQSSYLPYVTSRFVNAALK"
     gene            3713..4510
                     /gene="tauB"
                     /locus_tag="PKN55_09435"
     CDS             3713..4510
                     /gene="tauB"
                     /locus_tag="PKN55_09435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011136404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HNX71507.1"
                     /translation="MDDDALRAWSVSVRYPGQPEPALADVSLSIGPQALTVALGPSGC
                     GKTTLLNVLAGFVPPSQGRVTLAGAPITGPGADRAVVFQHDALLPWLDVRDNVAFALE
                     LQGVGRRERHARAEALLAEVGLAHAARQPVWTLSGGMRQRVGIARALASPSRALLMDE
                     PFGALDAFTREQMQTLLLEVWRHSGRRIFLITHDIEEALFLATELVLMSPGPGRIVER
                     IEPGFSRRWLAGEPVRALKSDPAFIALREHLLDTLARHRQAHDKETA"
     gene            4510..5346
                     /gene="tauC"
                     /locus_tag="PKN55_09440"
     CDS             4510..5346
                     /gene="tauC"
                     /locus_tag="PKN55_09440"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011136403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HNX71508.1"
                     /translation="MPSVFSSALPGVTGLSAPRWRLRLRHPLALGLFSVALLLLIWQI
                     AAGSGWIQPLFLPAPQAVLAKLALAAGQGFMDATLWQHLAASLSRIGLALLAAVAVGV
                     PLGLAMGVSPVLRGLLDPLIEGYRPVPPLAYLPLIVIWCGIGELSKVLLIFLAMLAPI
                     VLSATHGVTRVSPSRLRAAQSLGASRRQLLWRVVLPTALPDILTGLRIGLGAGWSTLV
                     AAELVAATRGLGFMVQSAAQFLVTDMVLAGIVVIGGIAFVLELGLRALQQRLCPWHGQ
                     QD"
     gene            5422..6255
                     /gene="tauD"
                     /locus_tag="PKN55_09445"
     CDS             5422..6255
                     /gene="tauD"
                     /locus_tag="PKN55_09445"
                     /EC_number="1.14.11.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011136402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
                     /protein_id="HNX71509.1"
                     /translation="MTLKLTRLSPALGAEVHDVDLSQPISEALLAELRAAWLQHQVLF
                     FRGQTLSPRQQRDFAARFGPLHTHPIYPQHPDAPEIMVLDTEAFDLRDNAIWHTDVTF
                     NATPPLGAVLAARKLPEVGGDTLWASGTAAFAALSPALQTLLTGLTATHDFVHSFPLE
                     RFGTRPEDLARWEQTRRDHPPVSHPVIRTHPETGQKALFVNDGFTTRINELSQEESDA
                     LLPLLFQHYARPEFTIRWRWQAGDVAFWDNRVTQHYATDDYRPAHRIMHRATIQGDVP
                     F"
BASE COUNT         1042 a         2179 c         2020 g         1093 t
ORIGIN      
        1 ccgccgccgg caggcgacgg ctgggctgac tgattacttc cagctttgcg gcagcttgta
       61 gccggcaaag cgggttttcg cccaggcttt gaattctgcc gagttgtaag ccgcggtgat
      121 gtccttcaca aaaggcttgt tgacgtcagc cgtgcgcacc accacccagt tcacgtaggc
      181 gtagcttttt tccagcgcca gcgcttcggt gaacttcatg cccgagctga ccgcgtaatt
      241 gccgttgacc acggcaaaat ccacgtcacc gcgcgcacgc ggcagttgcg cggcttccag
      301 cggcacaatc ttgatctgct tgaggttttt ttccacatcg cgttcgctgg cggtcagcgg
      361 atcaatgccc ggcttgaggg tgatccagcc caggtcggcc agcatcacca gcgcgcgcgc
      421 ctgattggtc ggatcgttgg gcaccgccac cgtgctgccg gccttcaccg ccgccagcgc
      481 cttttgcttg ccactgtaca ggcccagcgg gccggtcggc acttgcacca ccggggtcag
      541 cgccagcttg tgttctttgg cgaagttatc cagatagggc ttgtgctgga agatgttcac
      601 gtcgatcgag ccttcggcca gcgccagatt ggggcgcaca tagtcggtga attccaccag
      661 cttgaccttg tagccttgtt tttccaggat gggcttgatc gacagtttga ccatgtcgcc
      721 aaagtcgccg acggtggtgc cgaacaccag ctccggcttg gccgggtcgg cggccagggc
      781 ggaagtggac agcgcgccgc tcacggccag ggccacggcg agtttgagga aatgacgacg
      841 catgatgagc tccttatggg gtgaccggcg ggatggccgc ccggtcggtt tgccgccgtc
      901 cgggatgccg ggcggggcct gttcaaaatt ttgttcggtg ggcgattctt aacgcttgtc
      961 cagcgccttg gccagccgcg cccccaacca ctggatcagc tgcaccagca ccaccagcag
     1021 ggcgaccatc gccagcatca catcggtctg gaagcggtag tagccgtagc ggatggccag
     1081 atcgccaatg ccgccgccgc ccaccacccc ggccaccgcc gagtagctga gaaaactgat
     1141 ggtcagaatg gtcaggctgg acaccagtcc ggcgcgcgcc tcgttaatca gcaccttgcg
     1201 gatgatctgc cacgggctgg cacccatggc ttcggcggct tccagcacgc cgcgcggcac
     1261 ttcgcgcagg gtttgctcca ccagccgggc aaagtaagga atggcggcaa acgacagcgg
     1321 cacggcggcg gccaccgggc cgatggtgct gcccaccagc agccgggtca gcggcaccag
     1381 cgacaccatc aggatgatga acggaaacga gcgcaccaga ttcaccagcc agcccagcac
     1441 gccattcagc gcgcgatgcg cccacagctg ccccggctgg gtcaggaaca acacaatgcc
     1501 cagcggcccg cccagcacaa tggccaccgt caggccaatg cccagcatca gcgcggtttc
     1561 caggctggct tgccagattt ccggggccat cgacagcaaa ttactgaaat ccatctcacg
     1621 cctcctccag caaggccagg ccagccgcgc tgttcgcctg cgccacgccg tcaatcaccg
     1681 ggaagatttc ggcaatcgtc ccctgcgtca ccaccgccgc gcggtggcaa atggcgcgca
     1741 ccaccgccat ttcgtgggtg acgatcacca cggtcacccc caggcgctga ttgatgtcgg
     1801 ccaggcagtc cagaatgccg cgcgtggtgg ccgggtccag cgccgaggtc ggctcgtcgg
     1861 ccagcagcac atgcggacgc ggcgccagcg cgcgggcaat gcccacccgc tgcttttgcc
     1921 cgccggacaa ctgcgccggg taatcgccgg cgcggtccgc caggcccacc agcgccaggc
     1981 actccgccac ccgcgccgcg cgcacggcgg ccggccagcc ggcgatttcc agcggaaacg
     2041 ccacattgtc ggccaccgtg cggttggcca tcagattgaa ctgctgaaaa atcatgccga
     2101 tgttttgccg ggcccgacgc aagccggcgg cgggcagcgc ggtcagcgtc tggccgtcca
     2161 ccaccacctg gccgccatcc gggcgctcca gcagattgat caggcgcagc agggtggatt
     2221 tgccggcgcc agacgggccg atcagcccga agatttcccc ggcctgaatc tccaggtcgc
     2281 taggcgagag ggcggtgaac acgctgccgt ccgggcggac gaagcgcttg ctcacctgtt
     2341 tgagcgcaat cattgcattc tgctcctgcg gggtgtgccc cgcacgctgc atgccgcgcc
     2401 gggtggggcg cggcaaacca aaaaccgacg caggcggacg gggtaaccat cggcctgcgc
     2461 tatgatgggg ctattacaac tgtctgttta gtaaccgtaa aattgatttg gtcatggtgt
     2521 tattcctgtg ggaaatataa ccaaggccaa ctcagtctct agccctccca atctttagac
     2581 ctaaatctaa taaaaaaagc ttttcttatt ttcatatggc ttaatccggc cgcttgttac
     2641 agtgatttca cgcattcccg gcgctgggct cagcccaccg cgccgggcgc atcacctgaa
     2701 cttcactgga gcccgctcat gagtcatccc gccctgaaat ccctgtatgt cgccctgtcc
     2761 ctgctggcgg ggaccgccag cgccgccgac ccggtgacca tcgcctacca gaccggcatc
     2821 gacccctcca aggtggcgca ggccgatggc gcttacgaag ccgccaccgg cagcaagatc
     2881 aactggcgca aattcgaaag cggcgccgag gtgattgccg ccgtcgcctc gggcgatgtg
     2941 ccgattggca atatcggctc cagcccgctg gccgccgccg ccagccgtgg cctgccgatt
     3001 caaacctttt tggtgaccgc cgagattggc gattccgaag cattggtggt gcgcaacggc
     3061 agcaatatcg ccgccccgaa agacctggtc ggcaaaaaag tcgccgtgcc gtttgtctcc
     3121 accacccatt acagcctgct ggccgcgctc aagcactggg gggtggacgc cagcaaggtg
     3181 aacatcgtca acctgcgccc gagtgaaatc gccgccgcct ggcagcgcgg cgacatcgac
     3241 gccgcctacg tgtgggagcc ggcgctgggc acggccaagg ccagcggcaa ggtgctggtc
     3301 agctcggcgc aagtgggcaa gtggggcgcg ccgacttacg acctgtggat tgtgcgcaag
     3361 gactttgccg agaaaaatcc ggagtttctc acccagtttg tcaaagtcac cggcgccgcc
     3421 tacgccaagt acaacgccga cccgaagggc tacgccgcca acaacgccaa tgtggacaaa
     3481 atcgcccgcc tgaccggcgc caagccgcag gacgtggcgc tcttgctggc cggcaacacc
     3541 tatccgtcgc tcaagcgcca ggccgagctg ctggacaaac cgtttgccga ggcggtgggc
     3601 aaaaccgcgg aattcctgcg cgcgcaaggc aaggtggagc aggtgcaatc gtcttacctg
     3661 ccgtatgtca cctcgcgctt tgtcaacgcc gcgctgaagt aaggagcccg ccatggacga
     3721 cgatgcgctg cgcgcctggt cggtcagcgt gcgctacccc ggccagcccg agccggcgct
     3781 ggccgatgtc agcctgagca ttggcccgca ggcgctcacc gtggcgctgg ggccgtcggg
     3841 ctgcggcaaa accaccttgc tgaatgtgct ggccggtttt gtgccgccca gccaggggcg
     3901 ggtgacgctg gccggcgcgc ccatcaccgg gccgggcgcg gatcgcgccg tggtgtttca
     3961 gcatgatgcg ctgctgccct ggctggatgt gcgcgacaac gtggcttttg cgctggagct
     4021 gcaaggcgtg ggccggcgcg agcgccacgc ccgcgccgag gcgctgctgg ccgaagtggg
     4081 cctggcgcac gccgcccgtc agccggtgtg gaccttgtct ggcggcatgc gtcagcgcgt
     4141 cggcatcgcc cgcgcgctgg ccagcccctc gcgcgccttg ctgatggacg agccgtttgg
     4201 cgcgctggac gctttcaccc gcgagcagat gcaaaccctg ctgctggaag tctggcggca
     4261 cagcggccgg cggattttcc tgatcaccca cgacatcgaa gaagcgctgt ttctggccac
     4321 cgaactggtg ctgatgtcgc cggggccggg gcgcattgtc gagcgcatcg agccggggtt
     4381 cagccgccgc tggctggccg gcgagccggt gcgcgcgctg aaatccgacc cggcctttat
     4441 cgccctgcgc gaacacctgc tggacaccct ggcccgccat cggcaggcgc atgacaagga
     4501 gaccgcgtaa tgccttcggt gttttcttcc gccttgcccg gcgtcaccgg gctgagcgcg
     4561 ccgcgctggc ggctgcgcct gcgtcacccg ctggcgctgg gcctgttcag cgtggcgctg
     4621 ctgttgctga tctggcaaat cgccgcgggc agcggctgga tccaaccctt gtttctgccg
     4681 gccccgcagg cggtgctggc caagctggcg ctggccgccg gccagggctt tatggacgcc
     4741 acgctgtggc aacacctggc cgccagcctg tcgcgcatcg gcctggcctt gctggccgcc
     4801 gtggcggtgg gcgtgccgct gggcctggcc atgggcgtca gccccgtgct gcgtggcctg
     4861 ctcgacccgc tgattgaggg ctatcggccg gtgccgccgc tggcctatct gccgctgatt
     4921 gtcatctggt gcggcattgg cgagctgtcc aaggtgctgc tgatttttct ggccatgctg
     4981 gcgccgattg tgttgtccgc cacccacggc gtcacccggg tcagccccag ccggctgcgc
     5041 gccgcccaat cgctgggcgc cagccgccgt caactgctgt ggcgggtggt gctgcccacc
     5101 gcgctgcccg acatcctcac cggcctgcgc atcggcctgg gcgccggctg gtccaccctg
     5161 gtggccgccg agctggtggc ggccacccgt ggcctgggct tcatggtgca gtcggcggcg
     5221 caatttttgg tcaccgatat ggtgctggcc ggcatcgtgg tgattggcgg catcgccttt
     5281 gtgctggagc tgggcctgcg cgcgctgcaa cagcgcttgt gcccgtggca cggccagcag
     5341 gattgaatac ctcgcaattt tttagatcac ctattgtttt tccgcctggc cactggcgcg
     5401 ccaggcggca aggagccaag catgaccctg aaactcaccc gcctgagccc ggcgctgggc
     5461 gccgaagtgc acgacgtgga cctgtcgcaa ccaatcagcg aggcgctgct ggccgagctg
     5521 cgcgccgcct ggctgcaaca ccaggtgctg ttcttccgtg ggcaaaccct gtcgccgcgc
     5581 cagcagcgcg actttgccgc ccgctttggc ccgctgcaca cccacccgat ctacccgcaa
     5641 cacccggacg cgccggaaat catggtgctg gacaccgaag cctttgacct gcgcgacaac
     5701 gccatctggc acaccgacgt cacctttaac gccaccccgc cgctgggcgc ggtgctggcg
     5761 gcgcgcaagt tgccggaagt gggcggcgac acgctgtggg ccagcggcac ggcggcgttt
     5821 gccgcgctgt cgcccgcgct gcaaaccctg ctcaccggcc tgaccgccac ccatgacttt
     5881 gtgcactcct tcccgctgga gcgcttcggc acccggccgg aagacctggc gcgctgggag
     5941 caaacccgcc gcgaccaccc gccggtcagc cacccggtga tccgcaccca cccggaaacc
     6001 ggccagaaag cgctgttcgt caacgacggc ttcaccaccc gcatcaacga actcagccag
     6061 gaagaaagcg acgcgctgct gcccttgctg tttcagcact acgcccgccc ggaattcacc
     6121 atccgctggc gctggcaggc cggcgatgtg gcgttttggg acaaccgcgt cacccagcat
     6181 tacgccaccg acgactaccg cccggcgcac cgcatcatgc atcgcgccac catccagggc
     6241 gatgtgccgt tttgagcgtc atggcgactg aaggcaagcg gcggcgcgcc gtggtccgta
     6301 ctggatgccc gcctgcgcgg gcatgacgag gtgg
//
LOCUS       DAOPYX010000175         6319 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_15178_length_6319_cov_12.033685, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000175 DAOPYX010000000
VERSION     DAOPYX010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 6319)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6319)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6319
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15178_length_6319_cov_12.033685"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(212..763)
                     /locus_tag="PKN55_09450"
     CDS             complement(212..763)
                     /locus_tag="PKN55_09450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008954766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNX71510.1"
                     /translation="MKAIQLVLGLTLAATLAACSSTGGANGANGANGANGANGQLDPS
                     ASGYGRNGVGVGGDGSGSGDPLRDPKSILSKRSVYFDFDSSAVKGEYKPVVEAHAGYL
                     KKNPQRKAQIQGNTDAQGSREYNLALGQRRAESVKSMMRVLGVPENQLEAVSYGKEKL
                     RATGSSDADNAENRRADVAYDGE"
     gene            complement(793..2073)
                     /gene="tolB"
                     /locus_tag="PKN55_09455"
     CDS             complement(793..2073)
                     /gene="tolB"
                     /locus_tag="PKN55_09455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018746303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HNX71511.1"
                     /translation="MLRRLLHALAASLLLTLASLPARADMTIEIVGGGANRHVIALPA
                     FAAEAGVNGGITPIVRNDLQRSGAFNVLAPGVVANAPSSPGEIDYPAWRGAGAMSVAV
                     GRVSVSGGQLSVEFNLMDAAQKKRLTGGVVTVPVSDSRAAAHRIADMIYQAITGERGV
                     FSTQIAYVLRTGRSYQLQVADADGAGAQTLLRSGEPIISPAWSPDGSKLAYVSFETKK
                     PVVYVHALATGARYAVAAFKGSNSAPAWSADGSRLAVVLTRDGGSQIYSVPVAGGEAT
                     RLSQSAGIDTEPDYSPDGSRILFTSDRAGGPQIYSMSAGGGGASRVTFEGNYNVSPNF
                     APDGKSFTFIRREGGRYRVMVHDFTTGQANALTDTSRDESPSFAPNGKMILFATEQGG
                     RGVLYTVTRDGLTKTRLATTGGDVQEPAWGPFPR"
     gene            complement(2116..3417)
                     /gene="tolA"
                     /locus_tag="PKN55_09460"
     CDS             complement(2116..3417)
                     /gene="tolA"
                     /locus_tag="PKN55_09460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008954764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HNX71512.1"
                     /translation="MSERTPRHRLGFAVSLALHILLALVLTLSFSPEPVRVQAPLGVE
                     LWSAPPPMPGSAGAPALPAPAKPEPAPTPAPEPAPEPEPEPVAPPPAPPVKAAPAPRP
                     APVQAPEPPSARPDIAFKAKESKPEPKHKPEEKKPEPKKPEPKPEPKPEPKKPEPKPE
                     PKKPEPKPEPKKSEPKPEPKKSEPKPEPKPEPKKPEPKPEPKKPEPKPEPKPEPKKPE
                     PKPEPKKTESKPEAKPEPKKSDAKADSKADSKAEVKKPEAKPDGKSAEGKDAKAKGEG
                     KADAKSTQKGDGASASKTPAKANNPFADDLLADLNSSNSTHKPNAKTTQAGAAGGTPG
                     GGASGAKSSNGEYISQVVARVRPHVQVPDNVSGNPKAVVEVTLLPSLEVRDVKLLQSS
                     GNPAYDEAVQRAVWAAKTFPPLPPGAQFADFRRLKLEFRPR"
     gene            complement(3421..3831)
                     /locus_tag="PKN55_09465"
     CDS             complement(3421..3831)
                     /locus_tag="PKN55_09465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012698615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ExbD/TolR family protein"
                     /protein_id="HNX71513.1"
                     /translation="MLNRRPRRSMSQMNVVPYIDVMLVLLVIFMVTTPMFSPGVVNLP
                     SVGKAAQVESEPVQVLIDAQGNYSVSDKGSAHPVGDLAGLASQLETLTGGDKQRAVVI
                     SGDKTVAYDKVMAVMRELQQRGYARLGLLVKTES"
     gene            complement(3980..4165)
                     /locus_tag="PKN55_09470"
     CDS             complement(3980..4165)
                     /locus_tag="PKN55_09470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012698564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3460 family protein"
                     /protein_id="HNX71514.1"
                     /translation="MSMYQSDYTTFMNDFLQKNPSVAEDQQRLRLTWWDKQVDVNEQR
                     GFQEAKVQQKPYVYQPD"
     gene            4345..4935
                     /gene="ruvA"
                     /locus_tag="PKN55_09475"
     CDS             4345..4935
                     /gene="ruvA"
                     /locus_tag="PKN55_09475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HNX71515.1"
                     /translation="MIGRLSGRLLEKLPPHLLLDVQGVGYEVDAPMTTFYQLPAVGEA
                     VTLHIHQVVREDALLLYGFASRAERETFRQLLKVSGIGAKIALAILSSLSADELALAV
                     AGENVARLSKVPGIGKKTAERLILELRGKLGVAASDSPAALPMQAGNDASADITHALL
                     ALGYNDKEASAAVKTLPAGTKLEDGIRLALKALVKA"
     gene            4932..5591
                     /locus_tag="PKN55_09480"
     CDS             4932..5591
                     /locus_tag="PKN55_09480"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HNX71516.1"
                     /translation="MSRALFRRQPRTTPWLALGGVSGFALLALGFAHPPVSTLDAELG
                     QALFAWRDGALAQACVALARLFDGLGHAPVQAAWVALIAALLASLGGDRPAAGVLVMA
                     MLGQWGVNQLGKTLIDRPRPQLDPQLYTVGASFPSGHAMSATVLYGFLLLWLWPRCRP
                     GLRPWLALGAIGWIAAQALARPLLGAHYLSDVLAGVALGLACLAPAHAWLAQRDSHAF
                     A"
     gene            5602..>6319
                     /gene="ruvB"
                     /locus_tag="PKN55_09485"
     CDS             5602..>6319
                     /gene="ruvB"
                     /locus_tag="PKN55_09485"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HNX71517.1"
                     /translation="MIEPDALSAGPAPTRLVAAQTQSSQEDALERALRPRQLDEYVGQ
                     HKARGQLEIFIEAAKKRGEALDHVLLFGPPGLGKTTLAHIIARELGVNLRQTSGPVLE
                     RAGDLAALLTSLEPHDVLFIDEIHRLSPVVEEILYPALEDYQLDIMIGEGPAARSVKL
                     DLPPFTLVGATTRAGMLTNPLRDRFGITARLEFYSAEELTRIVTRSAGLLQVQIADDG
                     AHEIARRARGTPRIANRLLRR"
BASE COUNT          962 a         2044 c         2106 g         1207 t
ORIGIN      
        1 acaacgccag ggggttttgt gagcggctct tagtgtcgcc ataaggcaca gaaggggtca
       61 atctttgtgc agcattccat tgcgctgacg caagaataat cggcaaagcg cggcaagatt
      121 gccacggtac agaccgatta cgccaatttg ccggcaaaaa aatggccgaa gcaagatgct
      181 tcggccagat tggcgctagc gtggcgacga cttattcgcc gtcgtaggcc acgtcggcac
      241 ggcggttttc ggcgttatcc gcgtcgctgc tgccggtggc gcgcagtttt tccttgccgt
      301 agctgacggc ttccagctga ttttccggca cgcccagcac gcgcatcatg ctcttgacgc
      361 tttcggcgcg acgctggccc agcgccagat tgtattcacg gctgccctgg gcatcggtat
      421 tgccctggat ttgcgccttg cgttgcgggt ttttcttcag gtagccggca tgcgcctcca
      481 ccaccggctt gtattcgccc ttgaccgccg agctgtcaaa gtcaaagtac acgctgcgct
      541 tggacaggat gctcttcggg tcacgcagcg ggtcaccgct gccgctgcca tcgccgccca
      601 cgcccacgcc attgcggccg tagccggatg cgctcgggtc aagctggccg ttggcgccat
      661 tcgcgccatt ggccccgttt gcgccattgg cgccgccggt gctggagcac gccgccagcg
      721 tggcggccag cgtcaggccc agaacaagct ggatcgcttt cattgtttca ttctcctgtc
      781 gtgagggatg gttcagcgcg ggaaggggcc ccaggccggt tcctgcacat cgccgcccgt
      841 ggtggccaac cgggtcttgg tcaggccatc gcgggtgacg gtatagagta cgccgcgccc
      901 gccttgctcg gtggcgaaca agatcatctt gccattgggg gcaaagctgg gcgattcgtc
      961 gcgggaggtg tcggtcagcg cattggcctg gccggtggtg aaatcatgca ccatcacccg
     1021 gtaacggccg ccttcccggc ggataaaggt aaaagacttg ccatccgggg caaagttcgg
     1081 cgacacattg taattgccct cgaaagtcac ccggctggcg ccgccgccac cggccgacat
     1141 gctgtaaatc tgcgggccac cagcgcggtc agaggtaaac aggatgcgcg agccatccgg
     1201 cgaataatcc ggctcggtgt cgatgcctgc gctctggctc aggcgggtag cttcaccgcc
     1261 agccaccggc accgaataaa tctgcgagcc accgtcacgg gtcagcacca ccgccagccg
     1321 gctgccatcg gcgctccagg ccggggctga attactgccc ttgaacgccg ccaccgcata
     1381 gcgcgcgccc gtggccaacg cgtgtacata caccaccggt tttttggttt caaacgacac
     1441 ataagccagc ttgctgccat ccgggctcca ggccggcgag atgatcggtt cgcccgagcg
     1501 cagcagggtt tgtgcgccag cgccgtcagc atcggccact tgcagctgat agctgcggcc
     1561 ggtgcgcagc acgtaggcaa tctgggtgga gaacaccccg cgctcgccgg tgatggcctg
     1621 ataaatcatg tcggcaatgc gatgcgcggc cgcgcggctg tcgctgaccg gcacggtgac
     1681 cacgccgccg gtcaggcgtt ttttctgcgc ggcgtccatc agattgaact ccaccgacaa
     1741 ttgcccaccg ctgacgctga cccgccccac tgccacgctc atggcgcccg cgccgcgcca
     1801 ggccgggtag tcgatttcgc cagggctgct gggcgcattg gccaccacgc ccggcgccag
     1861 cacattaaaa gcgcccgagc gctgcaagtc gttgcgcaca atcggggtga tgccgccatt
     1921 gacgccagct tcggcggcaa aggccggcag ggcgatcaca tggcggttgg cgccgccgcc
     1981 gacgatttca atggtcatgt cggcgcgggc gggcaggctg gccagcgtca gcaacaggct
     2041 ggcggcaagg gcgtgcaaca gacgacgcag catggggttt cctcgggtaa gttctcggga
     2101 catgaataat gagcctcagc gtgggcggaa ctccagcttc aggcgacgaa aatcggcaaa
     2161 ctgcgcgcca ggcggcagcg gcggaaaggt cttggccgcc cacactgccc gctgcacggc
     2221 ctcatcgtaa gccggattgc cagacgattg cagcagcttg acgtcacgca cttccaggct
     2281 gggcagcagg gtgacttcga ccaccgcctt ggggttgccg ctgacattgt cgggcacctg
     2341 cacatgcgga cgcacccgcg ccaccacctg gctgatgtat tcgccattgc tgctcttggc
     2401 cccgctggcg ccaccgcctg gcgtgccgcc ggccgcgccg gcctgggtgg ttttggcgtt
     2461 gggcttgtgc gtgctgttgc tgctgttcag gtcggccagc aagtcatcgg caaacggatt
     2521 attggccttg gccggcgttt tgctggcgct ggcgccatca cccttttgcg tggatttggc
     2581 gtccgccttg ccttcgccct tggccttggc gtccttgccc tcggcggatt tgccatccgg
     2641 cttggcctca ggttttttca cctcggcctt gctgtcggct ttgctgtcgg ccttggcgtc
     2701 ggattttttt ggctccggct tggcttcggg cttggactcg gtttttttcg gctcaggctt
     2761 gggctcaggt ttcttaggtt ccggttttgg ctcgggctta ggctcaggct ttttcggttc
     2821 tggcttgggc tcgggtttct tcggttccgg tttcggctcc ggcttgggtt ccgacttttt
     2881 cggctccggt ttgggctcgg atttctttgg ctctggtttc ggctcaggct tcttgggttc
     2941 cggttttggc tccggtttct tcggctcggg tttaggctcc ggcttgggct cgggcttttt
     3001 cggttccggc tttttttctt ccggcttgtg cttgggctcg ggtttgcttt ctttggcctt
     3061 gaaagcgata tccgggcgcg ccgacggcgg ctcgggcgct tgcaccgggg ccgggcgggg
     3121 cgcaggcgcg gccttgactg gcggcgcagg cggcggggca acaggttctg gttccggctc
     3181 gggtgctggc tcgggggccg gtgttggcgc cggttcgggt ttggcgggcg ctggcaaggc
     3241 gggcgcacca gcgctgcccg gcatcggcgg cggcgccgac cacagttcga cacccaacgg
     3301 cgcttgcacg cgcaccggct cgggcgaaaa cgacagggtc agcaccaacg ccagcaggat
     3361 gtgcagcgcc agcgaaacgg caaaacccag gcgatgacgg ggcgtgcgct cactcaccgc
     3421 ttacgactcc gttttcacca gcagccccaa gcgggcgtag ccgcgttgtt gcagctcgcg
     3481 catcaccgcc atcaccttgt cgtaggccac ggttttgtcg ccagaaatca ccacggcgcg
     3541 ttgtttatcg ccgccagtga gggtttccag ctggctggcc aacccggcca gatcgcccac
     3601 cgggtgggcg ctgcccttgt cgctgacgct gtaattgcct tgcgcgtcga tcagcacctg
     3661 caccggctcg ctttccactt gcgcggcctt gcccacgctg ggcaggttga ccacgccagg
     3721 cgaaaacatc ggcgtggtga ccatgaaaat caccagaagc accagcatca cgtcgatgta
     3781 gggcacgacg ttcatctggc tcatcgagcg gcgggggcgg cggttcagca tgggcggtca
     3841 gcggcgggga gggcggaaag catgatcata cacgcgggag acagaagctt gaggactgcg
     3901 gccgggcaaa aaagttcgct ccaaaaggca acgcccggcc aagccgggcg aacctaccgc
     3961 agctggcgca gcagcaaaat cagtccggct ggtagacgta gggcttttgc tgcaccttgg
     4021 cttcttgaaa gccgcgctgc tcgttgacgt ccacttgctt gtcccaccag gtcaggcgca
     4081 ggcgctgctg gtcttcggcg accgacgggt tcttttgcag aaagtcgttc atgaatgtgg
     4141 tgtagtcgga ttggtacatg gacatggcgg gcggctcccg gttggcgaat gggcgctatt
     4201 gtaacggagt tggccggcgc tgccgatatt cgcgcgggtt tctgccgccc tgctgacatc
     4261 gcgccgggtg cgcaattgcg ctggctggct taccatttgc gccggcgcgc ccgcgcctga
     4321 ctcaaccccg gaaagcctga cgccatgatc ggacgcctga gcggccgcct gctggaaaag
     4381 ctccccccgc acttgctact ggatgtgcaa ggggtgggct acgaagtgga cgcgccgatg
     4441 accacctttt accaactgcc cgccgtgggc gaggcggtga cgctgcacat ccaccaagtg
     4501 gtgcgcgaag acgcgctgct gctgtacggc tttgccagcc gcgccgagcg cgaaacgttt
     4561 cgccagttgc tcaaggtcag cggcatcggc gccaagattg ccctggcgat tttgtccagt
     4621 ctgtcggccg acgaactggc gctggcggtg gccggcgaaa acgttgcccg cctgtccaag
     4681 gtgccgggca ttggcaaaaa aaccgccgag cggctgattc tggagctgcg cggcaagctg
     4741 ggcgtggcgg ccagcgacag cccggcggcg ctgccgatgc aggccggcaa cgacgccagc
     4801 gccgacatca ctcacgcctt gctggcgctg ggctacaacg acaaagaagc cagcgcggcg
     4861 gtgaaaaccc tgccggccgg caccaagctg gaagacggca tccgcctggc gctgaaagcg
     4921 ctggtgaagg catgagccgc gcgctgtttc gtcgccagcc gcgcacgaca ccctggctgg
     4981 cgctgggtgg ggtgagcggc tttgccctgt tggcgctggg gtttgcccac ccgccagtca
     5041 gcacgctgga cgccgagctg ggccaggcgc tgtttgcctg gcgcgatggc gcgctggcgc
     5101 aggcctgcgt ggcgctggcc cggctgtttg acgggctggg ccacgccccg gtgcaggcgg
     5161 cttgggtggc gttgattgcc gcgctgctgg ccagcctggg cggcgaccgc ccggcggccg
     5221 gcgtgctggt gatggcgatg ctgggccagt ggggggtgaa ccaactgggc aaaaccctga
     5281 tcgaccgtcc gcgcccgcag ctcgacccgc agctgtacac cgttggcgcg tcgtttccca
     5341 gcggccacgc catgagcgcc accgtgctgt atggcttttt gctgctgtgg ctgtggccgc
     5401 gctgccgccc cggcctgcgg ccgtggctgg cgctgggcgc catcggctgg attgccgcgc
     5461 aggcgctggc gcgcccgctg ttgggcgccc attatttaag cgacgtgctg gccggcgtgg
     5521 cgctgggcct ggcttgcctg gccccggcgc acgcctggct ggcgcagcgc gactcccacg
     5581 catttgcata aagacccgac gatgatcgaa cccgacgccc tttccgctgg ccccgccccc
     5641 acccggctgg tggccgccca gacccaatcc agccaggaag acgcgctgga gcgcgcgctg
     5701 cgcccgcgcc agctggatga atacgtcggc cagcacaagg cgcgtggcca gctggaaatc
     5761 tttatcgaag cggccaaaaa acgcggcgaa gcgctggacc atgtgctgct gtttggcccg
     5821 cccggcctgg gcaaaaccac gctggcgcac atcatcgccc gcgaactggg ggtgaacctg
     5881 cgccagacgt ctggcccggt gctggagcgc gcgggcgacc tggccgcgct gctgaccagc
     5941 ctggagccgc acgatgtgct gttcatcgac gaaatccacc gcctaagccc ggtggtggaa
     6001 gaaatcttgt acccggcgct ggaagactat cagctggaca tcatgattgg cgaaggcccg
     6061 gcggcgcgct cggtcaagct ggatttgccg cccttcaccc tggtgggcgc caccacccgc
     6121 gccggcatgc tgaccaaccc cttgcgcgac cgctttggca tcaccgcccg gctggagttt
     6181 tacagcgccg aagagctgac gcggattgtc acccgctcgg ccgggctgtt gcaggtgcag
     6241 attgccgacg acggcgccca tgaaatcgcc cgccgcgcgc gcggcacccc gcgcattgcc
     6301 aaccgcctgc tgcgccggg
//
LOCUS       DAOPYX010000176         6308 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_15219_length_6308_cov_9.580681, whole genome shotgun sequence.
ACCESSION   DAOPYX010000176 DAOPYX010000000
VERSION     DAOPYX010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 6308)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6308)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6308
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15219_length_6308_cov_9.580681"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(134..736)
                     /locus_tag="PKN55_09490"
     CDS             complement(134..736)
                     /locus_tag="PKN55_09490"
                     /inference="COORDINATES: protein motif:HMM:TIGR02292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein"
                     /protein_id="HNX71518.1"
                     /translation="MPAPLSDKDYQRLSATLTRLAPQGAMSLEHMDGFFTALLAGPEA
                     LSATATLPLVLGPAFDDDESFTSVKALEQFVDLVGRHWRDIADTLRNGAEFQPWLEED
                     AAGEVRGNDWAEGFSAGMQLFNDDWAEVFDHPQLGEALAPIMALAFERDPDPEMRPFL
                     GEVDEVRRAEWLGAIGPAVSALYGFFSARRAALEAEYGDD"
     gene            complement(890..1237)
                     /locus_tag="PKN55_09495"
     CDS             complement(890..1237)
                     /locus_tag="PKN55_09495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014086980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX71519.1"
                     /translation="MAKLSVTRGQTPAERARFALAESACQLADQLLGELASTEAAAAR
                     LRTLRVYWRKVRNGKVLSAADFNAAVDVCRSAERVLEALDAPALADPRLESLRRQLHA
                     MAEAWQALTGKPW"
     gene            complement(1237..4677)
                     /gene="dnaE"
                     /locus_tag="PKN55_09500"
     CDS             complement(1237..4677)
                     /gene="dnaE"
                     /locus_tag="PKN55_09500"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HNX71520.1"
                     /translation="MTTPRFVHLRLHSEFSITDGIVRLDEAVGRAVADGMPALGLSDL
                     ANLFGMVKFYKACRGKGVKPVVGADVWVENPDDADAPFRLLLLARNRDGYLTLCQLLT
                     DAYRTNQRHGRAEIKRDWLTPERCRTLLALSGAHMSDVGRALLDGQLPVAQAAARDWA
                     ARFGDGFYLEIQRYGAPHCDTLVERTLWLAGETGLPVVATHPVQFMHRDDHKAHEARV
                     CIAKGEILGDRRRARLFTEEQYFKSTAEMAELFADLPEALANSVEIAKRCNVTIQLGK
                     NYLPDFPTPDGMTLDDYLIHRANEGLVERMQALYPDPAQREAEMPRYRERLKFELDTI
                     IQMGFPGYFLIVADFINWAKHNGCPVGPGRGSGAGSLVAYSIGITDIDPLAYALLFER
                     FLNPERVSMPDFDIDFCQDNRWRVIEYVREKYGADAVSQIVTFGTMAAKAVVRDVGRV
                     LDLPYMFCDGLSKLIPAAPGKQYSLDDAVEMEPILKDRLAQEEEVAELWELAKKLEGL
                     TRNIGMHAGGVLIAPGKITDFCPMYQAGGEDASPVSQFDKDDVEQVGLVKFDFLGLRN
                     LTIIDLAVRYIKQLQPDFSVDMNTMPFTDPAAYKVLQEANTTAVFQLESDGMKKLLEK
                     LKPDRFEDIIAVLALYRPGPLGSGMVDDFILRKAGKQAVDYFHPDLTACLEPTYGVIV
                     YQEQVMQISQIIGGYTLGGADMLRRAMGKKKPEEMAKHRSIIAEGAKTKGYDPALAEQ
                     LFDLMTKFAEYGFNKSHTAAYAVVSYQTAWLKAHYPSAFMAATMSSELDNTDQLRVFY
                     DDSLKNKLKFLPPDINHSHYRFVPTDRQHIRYALGAIKGTGEAAVEHLIAEREAHGPF
                     VDLFDFCRRTDKRIVNRRAIETMIRAGAFDGLDDNRAKLLANVELAMSLAEQAAANIN
                     QGGLFDDFDDSVAPPVEMKDVPAWSEAQRLAEEKLAIGFYLSGHPFSAHAATVRRFVK
                     NTLASLSPKKEPQTLAGVVTLVRTKVGSRGKMAFVQLDDGTAKHDVSLFSEAYEANRH
                     KLREDALLIVEGKVSYDDFSGGLRIVVDSVLELAEAQSRFAQGLALSLHAESDIGKLK
                     HLLQPFAGSGPCPLRFRYAGSQARGELDAGEAWNVMLSDALLDSLRALLGEKSVEVLM
                     "
     gene            4966..6171
                     /locus_tag="PKN55_09505"
     CDS             4966..6171
                     /locus_tag="PKN55_09505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014086394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HNX71521.1"
                     /translation="MGTLPPNAADVLFLLLGAIMILAMHAGFAFLELGTVRKKNQVNA
                     LAKILSDFAVSAIAYFAVGYSVAYGVNFFAPASALTANHGYELVKFFFLLTFAAAIPA
                     IISGGIAERAKFSPQAFATFALVGLVYPFFEGIAWNNHFGLQDWLTTQFGAPFHDFAG
                     SVVVHAVGGWIALAAVLLLGPRHGRYHKSGSMSAHPPSSIPFLALGAWILIVGWFGFN
                     VMSAQKLDGMSGLVAMNSLMAMVGGTLTANWLGKNDPGFLHNGPLAGLVAVCAGSDVM
                     HPLGALAVGGAAGLLFVVMFTVTQNRWKIDDVLGVWPLHGLCGVWGGVACGLFGQTWL
                     GGVGGVSLASQALGTLAGVLIAFIGGGVVYGVLKATVGLRLDQEEEYQGADLTVHKIT
                     ATPEREASW"
BASE COUNT         1142 a         2001 c         1917 g         1248 t
ORIGIN      
        1 acaccatcag tagctttact ctaccgatga agaaaagctt agcatctctt gctagacaac
       61 cccatacttg catgtatttc atggcaaaca tgccacctca gcacccaaaa cccatatcag
      121 gcatggcaga ggctcaatcg tcgccgtatt ccgcctccag cgccgcgcgg cgggcactga
      181 aaaacccata caaggcgctg acagccgggc cgatggcgcc cagccactca gctcggcgca
      241 cctcgtccac ctcgcccaga aacggacgca tctccgggtc ggggtcacgc tcgaaggcca
      301 gggccatgat cggcgccagc gcctcgccca gttgcgggtg atcaaacacc tcggcccaat
      361 cgtcgttgaa cagctgcatg ccggcactga agccttctgc ccagtcattg ccgcgcacct
      421 cgcctgcggc gtcttcttcc aaccaaggct gaaactcggc gccgttgcgc aaggtgtcgg
      481 caatatcgcg ccagtggcgg cccaccagat cgacaaattg ttccagcgcc ttcaccgagg
      541 taaagctttc gtcatcgtca aatgccgggc caagcaccag cggcagggta gcggtggcgc
      601 tcagcgcctc cgggccggcc agcagggcag taaaaaagcc atccatgtgt tccagcgaca
      661 tggcgccttg cggggccagg cgggtgaggg tggcggacag gcgttggtaa tccttgtcgg
      721 acaagggcgc gggcatgcgg gcttccttag caagcaaatc agtttacaca gaatgggggg
      781 cggcttagag ccgctaacaa aaccccctgg cgttgttgtg tgaccttgcc gtactgcttg
      841 tactgtctgc ggtcacacaa taacgccagg agggttttgt acgcagcgcc taccagggct
      901 tgccggtcaa tgcctgccag gcttcagcca tggcgtgcag ctgacggcgc agcgactcca
      961 gccgggggtc ggccagcgct ggggcgtcca gcgcttccag cacacgctcc gcgctgcggc
     1021 acacatcgac agcggcgtta aaatccgctg cgctgagcac cttgccgttg cgcaccttgc
     1081 gccagtacac ccgcagggta cgcaggcggg cggcggcggc ttctgtgctg gccagttcgc
     1141 ccagcagttg atccgccagt tggcaagcgc tttccgccag ggcaaagcgg gcgcgttcgg
     1201 ccggtgtctg gccacgagtt acagataatt tggccattac atcagcacct caaccgattt
     1261 ttcgcccagc agggcgcgca ggctgtccag caaggcgtcg ctgagcatca cattccacgc
     1321 ctcgccagcg tccagctcgc cgcgcgcctg gctgccggca tagcgaaagc gcagggggca
     1381 ggggccgctg ccggcaaagg gttgcagcag gtgtttgagc ttgccgatat cgctctcggc
     1441 gtgcaggctc agcgccaggc cttgggcaaa gcggctttgc gcctcggcca gctccagcac
     1501 gctgtccacc acaatgcgca gcccgccaga gaaatcatcg tagctgacct tgccttcaac
     1561 aatcagcagc gcgtcttcgc gcagcttgtg gcggttggct tcgtaggctt ccgagaacaa
     1621 agacacatcg tgcttggcgg tgccgtcgtc cagctgcaca aaggccatct tgccgcgact
     1681 gcccaccttg gtgcgcacca gggtcaccac gccggccagg gtttgcggct ctttcttggg
     1741 cgacaggctg gccagggtgt ttttgacaaa gcggcgcacc gtggcggcat gggccgagaa
     1801 cggatggccg gacagataaa aaccaatcgc cagtttttct tccgccaggc gttgcgcttc
     1861 gctccaggcc ggcacgtcct tcatttccac cggtggggcc acgctgtcgt caaagtcatc
     1921 aaacagcccg ccctgattaa tattggcggc ggcctgttcg gccaggctca tcgccagttc
     1981 gacattggcc agcagtttgg cgcgattgtc gtccaggccg tcaaacgcgc cagcgcggat
     2041 catggtttcg atggcgcggc gattgacgat gcgcttgtcg gtgcgccggc agaagtcaaa
     2101 caagtccaca aacgggccat gggcctcgcg ctcggcaatc aggtgctcca ccgccgcctc
     2161 gccagtgccc ttgatggcgc ccagcgcata gcggatatgc tggcggtcgg tgggcacaaa
     2221 gcggtaatgg ctgtggttga tgtccggcgg caaaaacttc agcttgtttt tcagcgaatc
     2281 gtcgtaaaac acccgcagct ggtcggtgtt gtccaattca ctggacatgg tcgccgccat
     2341 gaacgccgac ggataatgcg ctttcagcca ggcggtctgg tagctgacca ccgcatacgc
     2401 ggccgtgtgc gacttgttaa agccgtactc ggcaaacttg gtcatcaggt cgaacaactg
     2461 ctcggccagc gccgggtcgt agcccttggt cttggcccct tccgcgatga tgctgcggtg
     2521 cttggccatt tcttccggct ttttcttgcc catcgcccga cgcagcatat cggcgccgcc
     2581 cagggtgtag ccgccgatga tctgcgagat ctgcatcacc tgttcctgat acacgatcac
     2641 gccataggtc ggctccaggc aggcggtcag gtcggggtgg aagtaatcca ccgcctgctt
     2701 gccggccttg cgcaagataa agtcgtccac catgcccgag cccagcgggc cagggcgata
     2761 cagcgccagc acggcgatga tgtcttcaaa acggtcgggc ttgagctttt ccagcagctt
     2821 tttcatgccg tcggattcca gctggaacac cgccgtggtg ttggcctcct gcaagacctt
     2881 ataggccgcc gggtcggtaa acggcatggt gttcatgtcg acgctgaagt cgggctgcaa
     2941 ttgcttgatg tagcgcaccg ccagatcaat gatggtgagg ttgcgcaggc ccaaaaagtc
     3001 gaatttcacc aggccgactt gttccacatc gtccttgtca aactggctga ccgggctggc
     3061 gtcttcgccg ccggcctggt acatcgggca aaaatcggtg atcttgcccg gcgcaatcag
     3121 cacgccgccg gcatgcatgc cgatattgcg ggtgaggcct tccagctttt tcgccagctc
     3181 ccacaactcg gcgacttctt cttcctgcgc cagtcggtct ttcaggatgg gctccatctc
     3241 caccgcgtcg tccaggctgt attgcttgcc cggcgcggcc ggaatcagct tggacaggcc
     3301 atcgcagaac atatacggca aatccagcac gcggccgacg tcgcgcacca ctgccttggc
     3361 ggccatggtg ccaaaggtga cgatctggct gaccgcgtcg gcgccgtatt tttcccgcac
     3421 gtactcaatc acccgccagc ggttgtcctg gcaaaagtcg atgtcaaagt caggcatcga
     3481 cacccgctcc gggttcagaa agcgctcaaa cagcagggcg taggccagcg ggtcgatgtc
     3541 ggtaatgcca atgctgtagg ccaccagcga gccggcgccc gagccccggc cggggcccac
     3601 cgggcagcca ttgtgcttgg cccagttgat aaagtcggcc acgatcaaaa agtaaccggg
     3661 gaagcccatc tggatgatgg tgtccagctc gaacttcagc cgctcgcggt agcgcggcat
     3721 ttctgcctcg cgctgggccg ggtcggggta gagcgcctgc atgcgctcga ccaggccttc
     3781 gttggcgcgg tggatcagat aatcatccag cgtcatgccg tccggggtgg ggaagtccgg
     3841 caggtagttc ttgcccagct gaatggtgac attgcagcgc ttggcgattt ccaccgagtt
     3901 ggccagcgct tccggcaggt cggcaaacag ctcggccatt tcggccgtgc tcttgaaata
     3961 ctgttcttcg gtaaacagcc gcgcgcggcg gcggtcgccg aggatttcgc ccttggcgat
     4021 gcacacccgc gcctcgtgcg ccttgtggtc atcgcggtgc atgaactgca ccgggtgggt
     4081 ggccaccact ggcaggccgg tttcgccggc cagccacagc gtgcgctcca ccagggtgtc
     4141 gcaatgcggc gcgccgtagc gctggatttc cagataaaaa ccatcgccaa agcgcgctgc
     4201 ccaatcgcgc gcggcggcct gcgccaccgg cagctggcca tccagcaagg cgcggccgac
     4261 atcgctcatg tgcgcgccgg acagggccag cagggtgcgg cagcgttcgg gcgtcagcca
     4321 gtcgcgcttg atttcggcgc ggccatggcg ctggttggtg cggtaggcgt cggtgagcag
     4381 ttggcacagg gtgaggtagc cgtcgcggtt gcgcgccagc aacagcaggc gaaacggcgc
     4441 gtcggcgtcg tccgggtttt ccacccagac atccgccccc accaccggct tcaccccctt
     4501 gccacggcaa gccttataaa acttgaccat gccaaacagg ttggccagat cgctcaggcc
     4561 aagcgccggc atgccgtcgg ccacggcgcg gccgacggcc tcgtccaggc ggacaatgcc
     4621 gtcagtgatg gagaattcag aatgcaggcg aagatgtaca aagcgcgggg tagtcatcgc
     4681 cgtattttac ccggttcgct gaccgctgtc ggcagcgatg cacgccggca acgcatgccc
     4741 attctggcgc gcagatttgc actatattcg tgcattgcgt tcagagttgg tgcgtagaac
     4801 ccacaataaa tgggcgaaaa tatggccaga ttagctttgt tacggtgcat taatctgatg
     4861 gcggacattt tttactttta ataaacaatg tattaatcac atcggcacta tgtttgcttt
     4921 cggtgcatcc atctgaaaaa cgcaccaata caaggaaagc cacccatggg aacgcttccc
     4981 cccaatgctg ccgacgtgct gttcttgctg ctcggcgcca tcatgattct ggccatgcac
     5041 gccggttttg cctttctgga gctgggcacc gtgcgcaaga aaaaccaggt gaacgccctg
     5101 gccaaaatcc tcagcgactt tgccgtctcc gccattgcct actttgccgt gggctacagc
     5161 gtggcctatg gcgtgaattt ttttgccccg gccagcgcgc tgaccgccaa ccacggttat
     5221 gagctggtga agttcttttt tctgctgacc tttgccgccg ccatcccggc catcatctct
     5281 ggcggcatcg ccgagcgcgc caagttcagc ccgcaggcct ttgccacctt tgccctggtg
     5341 ggcctggtgt atccgttttt tgaaggcatc gcctggaaca accattttgg cctgcaagac
     5401 tggcttacca cccaatttgg cgctcccttc catgactttg ccggctcggt ggtggtgcat
     5461 gccgttggcg gctggattgc cctggccgcc gtgctgctgc tcggcccgcg ccatggccgc
     5521 tatcacaaaa gcggcagcat gtcggcccac ccgccgtcca gcatcccgtt tttggccctg
     5581 ggcgcctgga ttctgattgt gggctggttt ggctttaatg tgatgagcgc gcaaaaactg
     5641 gacggcatgt ccggcctggt ggcgatgaac tcgctgatgg ccatggtggg cggcacgctc
     5701 accgccaact ggctgggcaa aaacgacccc ggctttctgc acaacggccc gctggccggt
     5761 ctggtggcgg tgtgcgccgg ctcggacgtg atgcacccac tgggcgcgct ggccgtgggc
     5821 ggcgctgccg gcctgctgtt tgtggtgatg ttcaccgtca cgcaaaaccg ctggaaaatc
     5881 gacgacgtgc tcggcgtgtg gccgctgcac ggcctgtgcg gcgtctgggg cggcgtggcc
     5941 tgcggcctgt ttggccaaac ctggctgggc ggcgtcggcg gcgtcagcct ggcctcgcaa
     6001 gcactgggca ccctggccgg cgtgctgatc gcctttatcg gcggcggcgt ggtgtacggc
     6061 gtactgaaag ccaccgtcgg cctgcgcctg gatcaagaag aagaatacca aggcgccgac
     6121 ctcaccgtgc acaagatcac cgccacacca gaacgcgaag ccagctggta atacacacca
     6181 tacggccgcc atcacgcaat cgggcggcca cgccagccgc ccgtttgcgt atctctgcgt
     6241 attgtttacg cctttatccg gttttaagct gagcgcttcg ccactgccga aagcccagca
     6301 tggatagc
//
LOCUS       DAOPYX010000177         6260 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_15390_length_6260_cov_11.194521, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000177 DAOPYX010000000
VERSION     DAOPYX010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 6260)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6260)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6260
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15390_length_6260_cov_11.194521"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            336..899
                     /gene="ahpC"
                     /locus_tag="PKN55_09510"
     CDS             336..899
                     /gene="ahpC"
                     /locus_tag="PKN55_09510"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HNX71522.1"
                     /translation="MSLINTQVQPFKAQAFHNGKFIEVTEESLKGKWSVLIFMPAAFT
                     FNCPTEIEDAANNYAEFQAAGAEVYIVTTDTHFSHKVWHETSPAVGKAQFPLVGDPTH
                     ALTNAFGVHIPEEGLALRGTFIINPEGVIKTLEIHSNEIARDVSETLRKLKAAQYTAA
                     NPGQVCPAKWKEGAATLTPSLDLVGKI"
     gene            1137..2705
                     /gene="ahpF"
                     /locus_tag="PKN55_09515"
     CDS             1137..2705
                     /gene="ahpF"
                     /locus_tag="PKN55_09515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019924562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016668 - oxidoreductase activity, acting
                     on a sulfur group of donors, NAD(P) as acceptor [Evidence
                     IEA];
                     GO_process: GO:0006805 - xenobiotic metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009398 - FMN biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HNX71523.1"
                     /translation="MLDDAIQAQLKAYLERLQRPIELVASLDDSANSQQMHTLLTQVA
                     ALSDKVSLRTDGDNPRRPSFGVAQPGDAPRIHFAGLPMGHEFTSLVLALLQTGGHPPK
                     VEQAVIDQIKALPGSFKFETFISLSCHNCPDVVQALNLMAVLNPGVTSTMIDGALFQD
                     EVNARQIMAVPTVYLNDAKFGQGRMTVEEVLAQVDTGASARAAAEISQKAPFDVLVVG
                     GGPAGAAAAIYAARKGIRTGVLAERFGGQVMDTLGIENFISVKETEGPKLAMALEQHV
                     KAYDVDVMNLQRAAKLIPASEAGGLIEVQLDNGATLKSRSVILATGARWREMNVPGEK
                     DYKAKGVCFCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVSHVTLLEFADTLRAD
                     AVLQNKLNSLPNVTVIKSAQTTEVTGDGQKVNGLSYKDRVSEEIKHIELEGIFVQIGL
                     LPATDWLKGTLELSRFGEIVIDAKGQTSAAGVFAAGDCTTAPYKQIIIAMGAGATASL
                     SAFDHLIRSSAPAA"
     gene            2705..3166
                     /locus_tag="PKN55_09520"
     CDS             2705..3166
                     /locus_tag="PKN55_09520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003111212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX71524.1"
                     /translation="MRALLVFLLLGSAALAHAAVLGVGDPLPKLRLATQHEQAASPAL
                     NARQWLFAADNDAANLAGALLDGQAASWLADTQRVYLADIHKMPALIARMVALPRLRD
                     KPYPILLGRDADTLAMLPRQRGCVSVLDIQQQKISAVHFACDQGGLAALVR"
     gene            3379..4698
                     /locus_tag="PKN55_09525"
     CDS             3379..4698
                     /locus_tag="PKN55_09525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX71525.1"
                     /translation="MPLSAAHRAALKQLYTALADRVLTPDDPVYIADLNRHGDADPIA
                     DIATEIDWQAGGGVCLFTGQRGTGKSTELQRLKRELEDLGARVFYVDLSEYLLLSKEV
                     EISDFLVSMAGAMSEQVQQQFIDTPGELGYWERFTQFLQSRVNIDEIKFKLPGAEIKA
                     ALKSDPDFKRHMQETMRGHIAQLVQETHAFFLQTVNFIRARIGQPDDGKVVLLVDSVE
                     RVRGVGNEAMKVYESVRNLFFSHAEHLCIPGLHVVYTVPPYLSVLAAGAGGLMGGATT
                     RRLVSTHIFQDRSRQPDPAGLATLRQVVSVRHPDWTALFTPAALDSLALSSGGDLREF
                     FRLIRLCLPAVRDDSQLPLDEAAVRSAQNAARNEMLPIPDNQLAWLQAITKTHSPCLK
                     TDADLPMLAHFLDNRMVLNYRNGSDWYDVHPLLREVVDNYDPAARLV"
     gene            4673..6214
                     /locus_tag="PKN55_09530"
     CDS             4673..6214
                     /locus_tag="PKN55_09530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNX71526.1"
                     /translation="MTPPPDWFDADEAQQLLRHLEWQPEGFSLLFVFADALPAQWLAD
                     WLNAALRPAGLTLQQPTCAQPLADAPDAVVDALMAELGALAQAPGAVWLPLHHHPQHA
                     DWDAARQRLLARLNERRFLLERDLPRPLLLVLPANFRPITREIAPDIWHIRVLSQQWR
                     TPALSAAQHWCWEKLPAGFPISRDRQPWMGRAEDADALLHEWKRSQKNEGNVALIETA
                     WQACAALIAAGRLSEAETILAPTLKAVLHNLKIPPKLDQDRSFALVHAFFQFISLATE
                     SERWGEVAGVYGLLLGFYKGSVESVGDESSIYLSAMGLYNLGVVNHQLGKPDSAAKKY
                     LLGLALAKEIYSSDVLWVKCCVQSNILYGQAKIATSSGDYPSALSTYQAALAHYEQAI
                     TITPSAAPALQRDLALIQVGLADAHMGLQDWPAAEQALAKSVAIFRQLAAAPRAPRHA
                     REDLAIALARQALLPPGDLAPLQEAERILLQLCAEFVEVPAYPRELSAVRELRRLVDA
                     ESQPG"
BASE COUNT         1219 a         2002 c         1846 g         1193 t
ORIGIN      
        1 gacgccaatc tgtcggaaag cgccaaggtg gaagtctctg ccggggtgta ttccggcacc
       61 acggtgaaca ttggcaagca atcaaccttt gtcaccgaag actctggttt tggcgtgttc
      121 cgtctggaag aaaaccagct gatcttcagc aaaacctgag cctgccctca cgcagccagc
      181 ctggccagat ggccatcgct ggctgctatc gccctgccag catcaatagc tgacatctat
      241 caagataatt atattaatca attggcttaa tcttcgacag ccgcctaaca tggtgttgta
      301 ccgtcaaccc cacccctgtt taggagactg tttcgatgtc gctgatcaat acccaggttc
      361 aaccgttcaa ggcccaagct ttccacaacg gcaagttcat cgaagtcacc gaagaaagcc
      421 tgaagggcaa gtggtctgtg ctgattttca tgccggccgc cttcaccttc aactgcccga
      481 ctgaaatcga agacgccgcc aacaactacg ccgaattcca ggctgctggc gccgaagtgt
      541 acatcgtcac caccgacacc cacttctcgc acaaagtctg gcacgaaacc tccccggccg
      601 tcggcaaggc ccagttcccg ctggtgggcg acccgaccca cgccctgacc aacgcctttg
      661 gcgtgcacat tccggaagaa ggcctggccc tgcgcggcac cttcatcatc aacccggaag
      721 gcgtgatcaa gaccctggaa atccactcca acgaaatcgc ccgcgacgtg tcggaaaccc
      781 tgcgcaagct gaaggccgcc cagtacaccg ccgccaaccc gggccaagtg tgcccggcca
      841 agtggaaaga aggcgccgcc accctgaccc cgtcgctgga cctggtcggc aagatctaag
      901 caccgcgaac aaaactctcc tggcgtggtt gcacgacctt gccgctgtct gccgcctggc
      961 caggatcgct acgctgagtt ttgtgagccg ctctcacgcc tgccccctgc cggcgccccc
     1021 acgccggttt tcgcgccagc ctcgcgctgg cgcggattgc accgccatgc gccgccagcc
     1081 cgcatcgccg tgcaatccgg tcgtccctta attacgtcaa gacaccagga gatcccatgc
     1141 ttgatgacgc catccaggcc caactcaaag cctacctcga acgcctgcaa cgcccgattg
     1201 aactggtcgc cagcctggac gacagcgcca acagccagca aatgcacacc ctgctgaccc
     1261 aggtggcggc gctgtccgac aaggtcagcc tgcgcaccga cggcgacaac ccgcgccgcc
     1321 cgtcgtttgg cgtggcccag ccgggagacg ccccgcgcat ccactttgcc ggcctgccga
     1381 tgggccatga attcacctcg ctggtgctgg ccctgctgca aaccggcggc catccgccca
     1441 aggtcgaaca agcggtcatc gaccagatca aggcgctgcc gggcagcttc aagtttgaga
     1501 ctttcatctc gctgtcctgc cacaactgcc cggacgtggt gcaagcgctg aacctgatgg
     1561 cggtgctcaa ccccggtgtg accagcacca tgattgacgg cgcgctgttt caggacgaag
     1621 tcaacgcccg ccagatcatg gccgtgccca ccgtctacct gaacgacgcc aaattcggcc
     1681 agggccgcat gaccgtggaa gaagtgctgg cccaggtgga caccggtgcc agcgcccgcg
     1741 ccgccgccga aatcagccaa aaagcgccgt ttgacgtgct ggtggtgggc ggtggcccgg
     1801 ctggcgccgc tgccgcgatt tacgccgcgc gcaaaggcat tcgcactggc gtgctggccg
     1861 agcgctttgg cggccaggtg atggacaccc tgggcatcga gaacttcatt tcggtgaaag
     1921 aaaccgaagg ccccaagctg gccatggcgc tggaacaaca cgtcaaggca tacgacgtgg
     1981 acgtgatgaa cctgcaacgc gccgccaagc tgattccggc cagcgaagcc ggcggcctga
     2041 ttgaagtgca actggacaac ggcgccacgc tgaaatcccg ctcggtgatt ctcgccacgg
     2101 gcgcgcgctg gcgcgaaatg aatgtgcccg gcgaaaaaga ctacaaagcc aagggcgtat
     2161 gcttctgccc gcactgcgac ggcccgctgt tcaagggcaa gcgcgtggcg gtgatcggcg
     2221 gcggcaactc cggcgtggaa gccgccattg atctggccgg catcgtcagc cacgtcaccc
     2281 tgctggaatt cgccgacacc ctgcgcgccg acgccgtatt gcagaacaag ctcaacagcc
     2341 tgcccaatgt caccgtgatc aaatcggcgc aaaccaccga agtgaccggc gacggccaga
     2401 aggtgaacgg cctgagctac aaagaccgcg tcagcgaaga gatcaagcac atcgagctgg
     2461 aaggcatttt tgtgcaaatc ggcctgctgc cggccaccga ctggctgaaa ggcacgctgg
     2521 agctcagccg ctttggcgaa atcgtcatcg acgccaaggg ccagaccagc gccgccggcg
     2581 tgtttgccgc cggcgactgc accaccgcgc cgtacaagca gatcatcatc gccatgggcg
     2641 cgggcgccac cgcctcgctg tcggcgtttg accacctgat ccgcagctcg gccccggccg
     2701 cctgatgcgc gcgctgctgg tcttcctgct gctgggcagc gccgccctcg cccacgccgc
     2761 cgtgctgggc gtgggcgacc ctctgcccaa gctgcgcctt gccacccagc acgagcaagc
     2821 cgccagcccg gcgctgaacg cccggcaatg gctgtttgcc gccgacaacg acgccgccaa
     2881 cctggccggc gccctgctcg acggccaggc cgccagctgg ctggccgaca cccagcgggt
     2941 gtatctggcc gacatccaca aaatgccggc gctgatcgcc cgcatggtgg cgctgccgcg
     3001 cctgcgcgac aaaccctacc ccatcctgct cggccgcgac gccgacaccc tggccatgct
     3061 gccgcgccaa cgcggctgcg tcagcgtgct ggacatccag cagcaaaaaa tcagcgccgt
     3121 gcactttgcc tgcgatcagg gcgggctggc ggcgctggtg cgttaagagc ggctaataaa
     3181 acctgcttgg tgttgttgtg cggccttgcc tacctgcggt cgcacgccca gccaggggcg
     3241 ctgcgctggg cttggggcct cacgctaaac gcacttcccg atgctttctg accgccgctg
     3301 ccgctacaat tcggggtatt gcctgcgcgc cagcttgctg cggcgcgcgc ctatcccttt
     3361 ttagtctgga gcgtatccat gcctctgtct gccgcccacc gcgctgccct caagcagctg
     3421 tacaccgcgc tggccgaccg cgtgctcacc ccggatgacc cggtgtatat cgccgacctg
     3481 aaccgccatg gcgatgccga cccgattgct gacattgcca ccgaaatcga ctggcaagcc
     3541 ggcggaggcg tatgtttgtt caccggccag cgcggcaccg gcaaaagcac cgagttgcaa
     3601 cggctcaaac gtgagctgga agatttaggc gcacgggtgt tttatgtcga tttatcggaa
     3661 tacctgctgc tgagcaaaga agtcgaaatc agcgattttt tggtgtcgat ggccggcgcg
     3721 atgtccgagc aagtgcaaca gcaatttatc gatacccctg gcgagctggg ctattgggag
     3781 cgctttaccc agtttttaca aagccgggtg aatatcgacg aaatcaaatt caaattaccc
     3841 ggcgccgaaa tcaaggcagc gctaaaaagc gaccctgatt tcaagcgcca tatgcaagaa
     3901 accatgcgcg gccatattgc ccaattggtg caggagaccc acgcgttttt cttacagacg
     3961 gtgaatttca tccgtgcgcg catcggccag cccgatgatg gcaaagtggt gttgctggtg
     4021 gattcggttg agcgagtgcg tggcgtgggc aacgaagcca tgaaggtgta tgaaagcgtg
     4081 cgcaatctgt ttttcagcca cgccgaacac ctgtgcattc ctggcctgca tgtggtgtac
     4141 accgtgccgc cttatttgtc ggtgttggcg gcgggcgcgg gcggactgat gggtggcgcc
     4201 accacgcgcc gcttggtgag cacccatatt tttcaagacc gcagccgcca gcccgacccc
     4261 gccggcctgg ccaccctgcg ccaggtggtc agcgtgcgcc accccgattg gacggccttg
     4321 tttaccccgg cggcgctgga cagcctggcg ttaagctcgg gcggtgattt gcgcgaattt
     4381 tttcgcctga ttcgtctgtg tctgccggca gtgcgtgatg atagtcagct gccgctggac
     4441 gaagcggcgg tgcggtcagc acaaaatgca gcgcgcaacg aaatgctgcc cattcctgat
     4501 aatcagctgg cctggttgca ggccatcacc aaaacgcact ccccttgcct gaaaaccgac
     4561 gccgacctgc ccatgctggc gcattttctg gataaccgca tggtgttgaa ttatcgcaat
     4621 ggcagcgact ggtacgatgt gcatccgctg ctgcgcgaag tggtggacaa ctatgacccc
     4681 gccgcccgat tggtttgatg ccgacgaggc acagcagtta ttgcgccacc tggaatggca
     4741 gccagaaggc ttttcgctgc tgtttgtgtt tgccgatgcc ctgcctgcgc aatggctggc
     4801 ggactggctg aacgccgcat tgcgcccggc gggcctgacg ttgcagcaac ccacgtgcgc
     4861 gcagccgttg gcggacgcac cggatgcggt agtggatgcg ctgatggccg aactgggcgc
     4921 gctggcgcag gcgcccggcg cagtgtggct gccactgcat caccacccgc aacacgccga
     4981 ttgggacgcc gcccgtcagc gcctgctggc gcggctgaac gaacggcgct tcttgctgga
     5041 acgcgacctg ccgcgcccgc tgctgctggt gctgcccgcc aattttcgcc ccatcacccg
     5101 cgaaatcgcc ccggatattt ggcatatccg ggtgctgagt cagcagtggc gcacgcctgc
     5161 gctgagcgca gcgcagcact ggtgttggga gaaactgccg gcggggtttc cgatttctcg
     5221 ggatcgccag ccatggatgg ggcgtgccga ggatgcggat gcgttgttgc atgagtggaa
     5281 acggagtcag aagaatgaag gtaatgttgc tctgatagaa accgcctggc aggcgtgtgc
     5341 agccttgatt gcagcagggc gattgtctga agcggaaact attttagccc ccacgttaaa
     5401 agcggtgctt cataatttga agatcccccc caagcttgat caagatcgtt cttttgcact
     5461 tgtgcatgcc ttttttcaat ttataagctt ggcaaccgaa tccgagaggt ggggtgaggt
     5521 tgcgggtgta tatgggttac tgcttggctt ttataaagga tccgttgaat cagtagggga
     5581 tgagtcaagc atttatttgt cggcgatggg attgtataat ttaggtgtgg ttaatcatca
     5641 gctaggcaag ccggatagtg cagctaagaa atatttgctt ggattggctt tagcaaaaga
     5701 aatttattcg tcagatgtat tgtgggtgaa atgctgtgtg caatcgaata ttctgtacgg
     5761 ccaagccaaa atcgccacct catccggcga ctacccctcc gccctgtcca cctaccaagc
     5821 cgcactcgcg cattacgaac aagctattac catcacccct tccgctgccc ccgccttaca
     5881 gcgtgacctc gccctaatcc aggtcggcct cgccgacgca cacatgggtt tgcaggactg
     5941 gcccgccgcc gagcaggcgc tggccaagag cgtggccatt ttccgtcaac tggctgccgc
     6001 gccgcgcgcg ccgcgtcatg cccgtgaaga tttggcgatt gcgctggcgc ggcaagcgtt
     6061 gctgccgccg ggcgatctgg cgccgttgca ggaagccgag cgtattttgc tgcaattgtg
     6121 cgccgaattt gtcgaagtgc cggcgtatcc gcgtgagttg agcgccgtgc gcgagctgcg
     6181 ccgtctggtg gatgccgagt ctcagcccgg ctgattcaaa ccatacttgc gcaccttgtc
     6241 aaacagcgtg gttttggcga
//
LOCUS       DAOPYX010000178         6246 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_15434_length_6246_cov_11.076724, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000178 DAOPYX010000000
VERSION     DAOPYX010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 6246)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6246)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6246
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15434_length_6246_cov_11.076724"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(68..1660)
                     /locus_tag="PKN55_09535"
     CDS             complement(68..1660)
                     /locus_tag="PKN55_09535"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNX71527.1"
                     /translation="MSFRLRIMSMVAGVLLLALIGLGYAALALQASQHSASRFFDHDL
                     KTLHAIDEMYAQGLQTGQALRNVILDHSNPQGHQNLASALKAFDIALASAREASPERA
                     ELWQQIDQLQQQRRRALEHTLQLAADQHAEAVATLNASETPAWRALRKVLLDARQSQR
                     QTAEQIKADTVADSRQAMHTVLVLGAVSLLVAAVLIQRVLASLKRELGGEPRAARQMM
                     RRVADGDLTVHIDAPAGSLLATLDDMVTALRTLLEAIDKQAVELALDSDETSRASASM
                     ADTAQQEALAAGALTDSVNAFLDSAARIAESAQSTRHDADAALTLSREGAQLAQSAAT
                     RMDSLSRTVSQAADTIQVLDQRADKIASMTSVIKDIADQTNLLALNAAIEAARAGEQG
                     RGFAVVADEVRKLAERTAQTTLEIDSLVNAIQHDTDAAVHAMHAALPEVDDGQKLASQ
                     AAGALDAIRDGAQRTVARVEDIAQASQGQTQASHAIAGQVQRVAAMVAETSDTIRQSA
                     INAAHLNGIAVALKTQVERFRM"
     gene            complement(2128..2583)
                     /locus_tag="PKN55_09540"
     CDS             complement(2128..2583)
                     /locus_tag="PKN55_09540"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNX71528.1"
                     /translation="MYRRILVAIDNSHGTPHVLDEALKLAGLHPAELHLVTVVNLLEL
                     AVEDPRQHDPAQLAALADQHGHTVLAPAKQHAGQHAGPLHTSVRHCWEGGKDIARALL
                     KHARDIHADLIIMGTHGRRGLRHALMGSVAEYVMRESHLPVLVVRTPRD"
     gene            complement(2666..3409)
                     /locus_tag="PKN55_09545"
     CDS             complement(2666..3409)
                     /locus_tag="PKN55_09545"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HNX71529.1"
                     /translation="MSDSAALPRLSKLMAERGLCSRREADSYIERGWVKVDGEVATLG
                     LRVSPDARIELLAQARGAQQERVTILLHKPVGYVSGQAEKGYQPAVSLILPERRWGGD
                     TAPLRFQREHLRGLAPAGRLDIDSTGLLVLTQDGRVAKTLIGEDSGVEKEYLVRVAGK
                     LSAEGLAKLNHGLSLDGQPLRPAKVWWQNDDQLRFVLREGKKRQIRRMCDLVGLRVLG
                     LKRIRIGKLMLGDLPQGQWRYLAPGERFD"
     gene            3601..5226
                     /locus_tag="PKN55_09550"
     CDS             3601..5226
                     /locus_tag="PKN55_09550"
                     /inference="COORDINATES: protein motif:HMM:NF024140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNX71530.1"
                     /translation="MLNQLSIRTRLAILAIMASLALLVLGSVNYWQTRQLTQALEQAS
                     AGQGKLRNQGEADMMHDAVRGDVLEMYYQASQANTTPATLDEVRKSFAEHAKDLREHV
                     AKNDALPLSSQERRELDALKPDLEAYLREADAALNTLASHGDARQVFDAFNQRFKALE
                     KSMDAFSTTLEREGQQEQQQASAQAERTSTNLVIALLLCMAGLITLDVLFGRSIVRSL
                     DDIRQFLAGLGNHLDRRLHSRGRDEVAEIARAIDSMLDKQADTIRMIQHAASTLRQTS
                     GQVSSQSQTASSRGADIAHTMSALTAAAEQMRAGIDAMARAIGGHAEEVRQANQANQH
                     AESAMLASRDASQGLVDATRQLGSMMSELNEAVGRIGAMAQVINDIADQTNLLALNAA
                     IEAARAGEQGRGFAVVADEVRKLAERTSSSTGDITAIIEAIRGSTEHAAQSMGALTSQ
                     VEDGLAALDQARLAQDTITGHMHNIHQVAGEVASATRQQAAAMAENAEGMQHIHGSLQ
                     VLQQVVEQLDVSASALAEEAQHLAGHASAFHTG"
     gene            5272..5571
                     /locus_tag="PKN55_09555"
     CDS             5272..5571
                     /locus_tag="PKN55_09555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008954678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SelT/SelW/SelH family protein"
                     /protein_id="HNX71531.1"
                     /translation="MSDPVVVRPRVEIRYCTQCRWLLRAAWMAQELLTTFDGDLGEVA
                     LIPATGGLFSVHVDGVLLWDRKTEDGFPDIKTLKQKVRDQIDPGRSLGHSDRPAN"
     gene            complement(5711..>6246)
                     /locus_tag="PKN55_09560"
     CDS             complement(5711..>6246)
                     /locus_tag="PKN55_09560"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNX71532.1"
                     /translation="AEKSQQISGIVAVIREIADQTNLLALNAAIEAARAGEQGRGFAV
                     VADEVRKLAERTAVATSEIAGMIQQVQDSASGAVDGMASAASQVGRGVDQANGASATM
                     GEIYNDLAAASDSVGVIAEVLHQQREATNVLSGNVELIARMAEENSAATRESASSAQR
                     LEELAGSLRDMASRFVV"
BASE COUNT         1107 a         1956 c         2030 g         1153 t
ORIGIN      
        1 agattacccg ctgccctgaa tgcggtctgt gagtgaggcg tgtcggaatg taaccacgtg
       61 tataccacta catccggaag cgctccacct gggttttcag tgccacagca atgccgttga
      121 ggtgggcggc attgatggcc gactggcgga tggtgtcgct ggtttcggcc accatggcgg
      181 ccacccgctg tacctggccg gcaatggcgt ggctggcttg ggtttgcccc tggctggcct
      241 gggcgatgtc ttccacccgt gccactgtgc gttgggcgcc atcgcggatg gcatccagcg
      301 cccctgccgc ctggctggcc agtttctggc cgtcgtccac ttccggcaga gcggcgtgca
      361 tggcgtgcac ggcggcatcg gtatcgtgct ggatggcgtt gaccaggctg tcgatttcca
      421 gcgtggtctg ggcagtacgt tcggccagct tgcgcacttc gtcggccacc acggcaaagc
      481 cgcgcccttg ctcgccagca cgggcagctt cgatggcagc gttcagcgcc agcagattgg
      541 tctggtcggc gatatccttg atgaccgagg tcatgctggc gatcttgtcg gcgcgctgat
      601 ccagcacctg gatggtgtcg gcggcctggc tgaccgtgcg cgacaggctg tccatgcgcg
      661 tggcggcgct ttgcgccagt tgggcgcctt cgcgtgacag ggtcagcgcg gcgtctgcgt
      721 cgtggcgggt ggattgcgcg ctctcggcaa tgcgcgcggc gctgtccaga aaagcattga
      781 cgctgtcggt cagtgcgccg gccgccaggg cttcctgctg ggcggtgtcc gccatgctgg
      841 ccgaggcccg gctggtttca tcgctatcca gtgccagctc caccgcctgt ttgtcgatgg
      901 cttccagcag ggtgcgcagc gcggtaacca tgtcgtccag agtggccagc aggctgccgg
      961 ccggggcgtc gatatgcacg gtcaggtcgc cgtcagccac ccggcgcatc atctgccgtg
     1021 cggcacgcgg ttcgccgccc agctcgcgct tgaggctggc cagcacccgc tggatcagta
     1081 cggcagccac cagcaggctg accgcgccca gcaccagcac ggtgtgcatg gcctgccggc
     1141 tgtcggctac ggtatcggcc ttgatctgtt cggcagtctg gcgctggctt tgccgggcat
     1201 ccagcagcac tttgcgcaag gcacgccagg ccggggtttc actggcattc agcgtggcga
     1261 cggcttcggc gtgctgatcg gccgccagtt ggagagtgtg ttccagtgcg cggcggcgct
     1321 gctgttgcaa ctggtcgatc tgctgccaga gttcggcgcg ttcggggctg gcttcgcgcg
     1381 cgctggccaa ggcgatgtca aaggctttca gcgcagaagc caggttttga tggccctgcg
     1441 ggttgctgtg gtcaagaatg acattgcgca gcgcctggcc ggtttgcaat ccctgcgcgt
     1501 acatttcgtc gatggcgtgc agggttttca ggtcgtggtc aaaaaagcgg ctggcgctgt
     1561 gctggctggc ttgcaaggcc agggcggcgt agcccagtcc aatcagggcg agcagcagca
     1621 caccggccac catgctcatg atgcgaagac gaaagctcat gtgaaagtcc gtgaagtcaa
     1681 cgctggccac cgggttttcg cgtgacgcat caggccgggc gtgctggcac aggcacagcg
     1741 cgccgagcag ccaggctgct tcgccacacg ggaacatacg gtaaacagca ccaggaacgg
     1801 cggacaaacc ccgcgcagcg gttcaggcca gtcagcgcgg cccacaaagc ccgtctggcg
     1861 ggcttgtgtc accgatatgg tggtggtcac acgcctggcc aggaccgttg cgctgagtgt
     1921 tgtaggcaat gcttgaggat gggtcagccg ttccaagggg ccagcgcagc ccgctgattc
     1981 atggtcaggc agaccatcag gcaaggcatg cgctcgggtg ccagcctgcg cctacgaagc
     2041 gcgctccgta tcccggctga cgttctgttt tatgttttga cagattgtag caggattcca
     2101 caagcacaga agggtttttg ccaaatatca gtcacgcggg gtgcgcacca ccagcaccgg
     2161 caggtggctt tcccgcatca catattcggc cacgctgccc atcaacgcat ggcgcaaacc
     2221 acgccggccg tgggtgccca tgatgatcag gtcggcatgg atgtcgcggg catgcttgag
     2281 cagggcgcgg gcaatgtcct tgccgccttc ccagcagtgc cgcacgctgg tgtgcagcgg
     2341 gccggcatgc tggccggcgt gttgcttggc cggggccagc acggtgtggc catgctgatc
     2401 ggccagcgcg gccagttgcg ccggatcgtg ctggcgcggg tcttctacgg ccagctccag
     2461 cagattgacc acagtgacca gatgtagctc ggccgggtgc agaccagcca gtttcagtgc
     2521 ctcatccagc acatgcggcg tgccgtggct attgtcgatg gcaaccagga tgcggcggta
     2581 catgacaaac tcctgtcaac gggagaaatt cggaatgcgc tggccacggc aagctgcagg
     2641 ccagcttggg ctggtggacc catcctcagt cgaaacgttc gcctggcgcc agatagcgcc
     2701 actggccctg cggcaggtcg cccagcatga gcttgccgat acggatgcgc ttgaggccca
     2761 acacgcgcag gcccaccaga tcgcacatgc ggcggatctg gcgctttttg ccttcgcgca
     2821 gcacaaagcg cagctgatcg tcgttttgcc accacacctt ggccgggcgc agcggctggc
     2881 catccaggct caggccgtgg ttaagcttgg ccagtccttc ggccgacaat ttgccggcca
     2941 cccgcaccag atattctttt tcgacgccgg aatcttcgcc aatcagcgtt ttggccaccc
     3001 ggccatcctg ggtcagcacc aagagcccgg tggaatcgat atccagccgg ccggccggag
     3061 ccaggccgcg cagatgttcg cgctgaaagc gcaacggcgc cgtatcaccg ccccagcgcc
     3121 gctcgggcag gatcaggctg acagcgggtt gatagccttt ttccgcctgg cctgagacat
     3181 aacccaccgg cttgtgcagc aggatggtca cccgctcttg ctgggcgccg cgcgcctggg
     3241 ccagcaactc gatgcgggcg tccgggctga cgcgcaaccc gagggtggcc acttcaccat
     3301 ccactttcac ccagccgcgc tcgatatagc tgtctgcttc gcggcgcgag cacagaccgc
     3361 gttcggccat cagtttggac aggcggggca gggcggcgga gtcgctcatg gacggaaact
     3421 ttcgtgaaca aaaccgcatt gtagcgcagc catcacgaca aattacccgg gcatcagcca
     3481 tgccaggctg acaaaccggg cagccatggc ataccattca actaaatcac gctggatatt
     3541 gatcaaaatc aaacgattaa tcgcttgttg cctttgtgtg caaaggccgg agatatcgcc
     3601 atgctgaacc aattgagtat ccgcacccgc ctggccattc tggccatcat ggcatcgctg
     3661 gccctgctgg tgctgggctc ggtgaactac tggcagacgc gccagctgac ccaggcactg
     3721 gagcaggcat cggccggcca gggcaagctg cgcaaccagg gcgaggccga catgatgcat
     3781 gacgcggttc gcggcgatgt gctggaaatg tattaccagg ccagccaggc caataccacg
     3841 ccggccacgc tggacgaggt gcgcaaatcg tttgccgagc atgccaagga tttgcgcgaa
     3901 catgtcgcca aaaacgacgc gctgccgctg tcgtcccagg agcgccgcga gctggatgcg
     3961 ctcaagccgg atctggaagc ctatctgcgc gaggctgacg ccgcgctcaa caccctggcc
     4021 agccacggcg atgcccgtca ggtattcgat gccttcaacc agcgtttcaa ggcgctggaa
     4081 aaatccatgg atgccttcag caccacgctg gagcgcgagg gccagcaaga gcagcagcag
     4141 gccagcgccc aggccgagcg caccagcacc aatctggtga ttgccctgct gctgtgcatg
     4201 gcggggctga ttaccctgga tgtgctgttc ggccgcagca tcgtgcgttc gctggacgat
     4261 atccgccagt ttctggccgg ccttggcaat catctggacc ggcgcttgca tagccgtggc
     4321 cgtgatgaag tggcggagat agcccgcgcc atcgacagca tgctggataa gcaggctgac
     4381 accatccgca tgatccagca tgcagccagc accctgcgcc agacttccgg tcaggtgtcg
     4441 tcacaatcgc agaccgccag ctcgcgcggg gccgatatcg cccacaccat gtcggcactg
     4501 actgccgccg ccgagcagat gcgcgccggg atcgacgcca tggcgcgggc gattggcggc
     4561 catgccgaag aagtgcgtca ggccaatcag gccaaccagc atgctgaatc agccatgctg
     4621 gcctcgcgtg atgccagtca ggggctggta gacgccaccc gccagctggg cagcatgatg
     4681 agcgagctga acgaagcggt ggggcgtatc ggggccatgg cgcaagtgat caacgacatc
     4741 gccgatcaga ccaatctgct ggccttgaac gcggcgattg aagccgcccg tgccggcgag
     4801 caagggcggg gttttgccgt ggtggccgat gaggtgcgca aactggccga gcgcaccagc
     4861 agcagcaccg gcgatatcac cgccattatt gaggcgatcc ggggctcgac cgagcatgcc
     4921 gcacagtcga tgggggcgct gaccagccag gtggaagatg gcctggccgc gctggatcag
     4981 gctcgtctgg cccaggacac cattaccggc cacatgcaca atatccatca agtagcaggc
     5041 gaagtggcca gcgccacccg ccagcaggcg gcggccatgg cagaaaacgc cgaaggcatg
     5101 caacatattc atggcagctt gcaggttttg cagcaggtag tggagcaact ggatgtctcg
     5161 gccagcgccc tggctgaaga agctcagcac ctggccggcc atgcgtcggc gtttcatacc
     5221 ggctaggcca cgtcctcacg cttataatgg cgttttgccg gagcccttgc catgtctgat
     5281 cccgttgttg tccgccccag ggtggaaatc cgctattgca cccagtgccg ctggttactg
     5341 cgcgccgcct ggatggcgca ggagctgctg accacgtttg acggggattt gggcgaagtg
     5401 gcgttgattc cggccacggg tggcttattc agtgtgcacg tggatggcgt gttgctgtgg
     5461 gaccgcaaaa ccgaagacgg ctttcccgat atcaagacac tcaagcagaa agtgcgcgac
     5521 cagatcgacc ctggccgctc gctggggcac agtgaccggc cggccaatta gcgcggagaa
     5581 caaaactctc ctggccgtac tggcgcatcg cgggtcttgt ccgccgagtg tgattcgcgg
     5641 cactcaatga aaaataaaaa cccccgcgcc atgcactggc gcggggggtt tttattgagc
     5701 ggcttgcggg ttacaccacg aagcggctgg ccatatcgcg cagcgagccg gccagttctt
     5761 ccaggcgctg ggccgaactg gcgctttcgc gggtggccgc gctgttttct tcggccatgc
     5821 gggcaatcag ttcgacattg ccggacagca cattggtggc ttcgcgctgc tggtgcagta
     5881 cttcggcaat cacgcccacc gaatccgagg cagcagccag atcgttgtaa atctcgccca
     5941 tggtggcgct ggcgccattg gcctgatcca caccacggcc cacctggctg gcggcgctgg
     6001 ccatgccatc cactgcgcca gaggcgctgt cctgcacctg ctggatcatg ccggcaattt
     6061 cgctggtggc cacggcggtg cgttcggcca gcttgcgcac ttcgtcggcc accacggcaa
     6121 agccacggcc ctgctcaccg gctcgggcgg cttcaatggc ggcgttcaac gccagcagat
     6181 tggtttggtc agcgatttca cgaatcacgg cgacaatgcc ggaaatctgc tgggatttct
     6241 cggcca
//
LOCUS       DAOPYX010000179         6227 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_15514_length_6227_cov_7.479099, whole genome shotgun sequence.
ACCESSION   DAOPYX010000179 DAOPYX010000000
VERSION     DAOPYX010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 6227)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6227)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6227
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15514_length_6227_cov_7.479099"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..574)
                     /locus_tag="PKN55_09565"
     CDS             complement(<1..574)
                     /locus_tag="PKN55_09565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008953869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HNX71533.1"
                     /translation="MSDLASAQLLTASAAQLPVYTYFDPAYYELEQQLLFADAPQYYG
                     HALMTPNVGDYHTLEWMGHGKMLKRNEDGIQLLSNVCRHRQALMLKGRGNTEHIVCNI
                     HGWTYNNQGKLIGAPHFHQQPCLDLGQTQLTEWNGLLFDARRDIAADLARLGVAKHMT
                     FDGYAYHETVITEYNFNWKTFIEVYSEDYHV"
     gene            complement(684..995)
                     /locus_tag="PKN55_09570"
     CDS             complement(684..995)
                     /locus_tag="PKN55_09570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX71534.1"
                     /translation="MVGHQHIRSSETLARQFGHKRAKTRKNSPLRGGRDRLPELYRAG
                     MLRRNKILGRLICRKFCPENFCHPRKKPGKTAKSIDFAQFILQKTLAPHPDFGDNPPP
                     S"
     gene            984..2171
                     /locus_tag="PKN55_09575"
     CDS             984..2171
                     /locus_tag="PKN55_09575"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003776902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HNX71535.1"
                     /translation="MTDHVLVINCGSSSLKFAAIDPRTGVAALTGLAERLGSPEASLS
                     ARLGEDRHGVTLAGGGHEAAMRAVLDVLDASGLTTRIGAVGHRVVHGGERFKSSVRID
                     DAVEAEIDACARLAPLHNPANLIGVRVARRCFPSLPHVAVFDTAFHQSMPAHAYLYAI
                     PHSLYAEHGLRRYGFHGTSHRYVAAEAAQLLGRPLQDCALITAHLGNGASAAAVLNGH
                     SVDTTMGLTPLEGLVMGTRSGDIDPSLVQFIGNALNQSADQVIDLLNRKSGLLGLSGL
                     SNDCRTLQAAAEEGHAGARVALEVFCYRLAKHIAGLSAALPRLDALVFTGGIGENSAW
                     VREQVLARLGLLGFAVDSAANARCVGGVAGPIHAAGSRTVLVVNTNEELLIARDTFAL
                     AGV"
     gene            2333..4426
                     /gene="pta"
                     /locus_tag="PKN55_09580"
     CDS             2333..4426
                     /gene="pta"
                     /locus_tag="PKN55_09580"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014086784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HNX71536.1"
                     /translation="MHALYLSPVGYDVGLTSVALGLVRALERRGLKVGFIKPVAQQRD
                     PGAGPERSSHFASSLFRLDTPPPIDFARAEQLMGAGQTDQLLEEIVSLYQQVVDHNDV
                     VVVEGLAADANHVYAPQLNTQIARTLNAETIFVGSAARLSAQELAEQIEVAARQSAES
                     HNNRFAGYIINKVREDVAPGDYTLDIAQLTATAKARRLPLLGTIPLSSNFAAPRTLDI
                     AQHLNARVLAPARLHSARVSDMLIAARSITHLINRLKPGALVITPGDRDDVVLATAMA
                     AQRGVPLAGLLLTCNSLPHPHLQALCQINEHASFPVLATELETFDTARQLTQMSHHVP
                     VDDLARMERVVDFVAERLDLDRLISRIGQPGEVRLSPPAFRYQLIQHARRAGKRIVLP
                     EGDEPRTVEAAVICHQKGIARCVLLAKPEAVQKVADAQGITLPADLEILDPDHARARY
                     VPPLVELRRHKGMTAHMAEQYLEDSVVLGTMMLAVNDVDGLVSGAVHTTANTIRPALQ
                     FIKTAPGSSIVSSVFFMLMPDQVLVYGDCAVNPDPSAEELADIAIQSADSAKAFGISP
                     RVAMISYSTGSSGAGSDVEKVRAATELVRQRRPDILVDGPLQYDAASVESVGRQKAPD
                     SPVAGRANVFVFPDLNTGNTTYKAVQRSANVVSVGPMLQGLRKPVNDLSRGALVDDIV
                     YTIALTAIQAEQMAN"
     gene            complement(4527..6080)
                     /locus_tag="PKN55_09585"
     CDS             complement(4527..6080)
                     /locus_tag="PKN55_09585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014086518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HNX71537.1"
                     /translation="MKTRLLKGSVAGVLGIAVLYSAGAYWTGLEAERTLAKQHDMIAG
                     LPIFKVKSHQYERGWFSSTETTELTFNERFFAPYQGILPEAAKNALTGTVKYVNHIQH
                     GPLPGGLRPARAVVTTEFIMSPDTRKMLSRFFGDKEPITVTNRLNFTGGGELNVSVPA
                     FDYEETLAGVKIKWQGMTTQVGYDSDYSQYSIDARLPGLVLDAATKGHIEFSNLSYQS
                     DTRPGATGAGVGNKELSVTRVKLESKENIPYQIKLNELISLLTRIRIGEFINPAGEIK
                     PSQADLVNLKYQIVSSEEKEFINARAKFGFEKLEVNHTAYGPMRLDLSVNHLHGPSLL
                     KLEQAFSDIQVEGVEPDKLRQQYIDAVVREGGPILENNPRLAINDFSLKLPSGEVTVT
                     GNVALNGYQKGDLNDSRVFLSKLTANAKLSVPRDTLQGLVVAQARNLFTVDASAENPP
                     SLQEIDELAKNLFASQVDIWSEQGYLQQEGGQLKTSAEWKNGKLTINNRPVNLPDPAA
                     VEAAAAPKP"
BASE COUNT         1130 a         2006 c         1898 g         1193 t
ORIGIN      
        1 caacgtggta atcctccgaa tacacttcga taaaggtttt ccagttgaag ttgtattcgg
       61 tgatgacggt ttcgtgatag gcgtagccat caaacgtcat gtgcttggcc acgcccaggc
      121 gcgccaggtc ggcggcgatg tcgcggcggg cgtcaaacaa caggccgttc cattcggtca
      181 gctgggtctg acccaggtcc aggcacggct gctgatgaaa atgcggcgcg ccaatcagct
      241 tgccctggtt gttgtaggtc cagccatgga tattgcagac gatgtgttca gtgttgccgc
      301 gccccttgag catcaaggcc tggcgatggc ggcagacatt ggacagcaat tgaatgccgt
      361 cttcgttgcg cttgagcatc ttgccatggc ccatccactc cagggtgtga tagtcgccca
      421 cgttcggcgt catcaaggca tggccatagt attgcggcgc gtcggcgaac agcagttgct
      481 gttccagctc gtaatacgcc ggatcaaaat aggtatagac aggaagttgc gcggcggatg
      541 cggtaagcag ttgcgcagaa gccagatcag acattattcc caatccccac aaaggtagaa
      601 gaatcaagac cagccgaatc tcgccaaaac gagtttacgg tatgaaggca caaacagttg
      661 caggcgcaca cgccagacag ccgtcaggaa ggcgggggat tgtccccaaa atcggggtgg
      721 ggggcaagcg ttttttgcaa tataaattgt gcaaaatcaa tcgattttgc cgttttgccc
      781 ggcttcttcc gggggtggca gaagttttct ggacaaaact tgcgacaaat caaacgtcca
      841 agtattttgt tgcgacgcaa catccccgcg cggtatagtt cgggcagcct gtcccttcca
      901 ccgcgcagcg gactgttttt tcttgttttt gcgcgcttgt gcccgaactg gcgcgccagc
      961 gtttctgaag agcggatgtg ctgatgaccg accatgttct tgtgattaat tgcggtagtt
     1021 cctcgctgaa atttgccgcc atcgacccac gcaccggtgt cgccgcgctg accggcctgg
     1081 ccgaacgact gggtagcccg gaagccagcc tgagcgcccg actgggcgaa gaccgccacg
     1141 gcgtgaccct ggccggtggc ggccacgaag ctgccatgcg cgcggtgctg gacgtgctgg
     1201 acgccagcgg actgaccacc cgcatcggcg cggtgggcca ccgcgtggtg cacggcggcg
     1261 agcgctttaa aagctcggtg cgcatcgacg acgccgtcga agccgaaatc gacgcctgcg
     1321 cccgtctggc gccgctgcac aacccggcca atctgattgg cgtgcgcgtg gcgcgccgat
     1381 gctttcccag tttgccgcat gtggcggtat tcgacaccgc gtttcaccag agcatgccgg
     1441 cgcatgccta tctgtacgcc attccacaca gcctgtacgc cgaacacggc ctgcgccgct
     1501 atgggtttca tggcaccagc caccgttatg tcgccgccga agccgcccag ctgctgggcc
     1561 gcccgctgca agactgcgca ctgatcaccg cccacctggg caatggcgcc tcggccgccg
     1621 ccgtactgaa tggccacagc gtggacacca ccatgggcct gaccccgctg gaaggcctgg
     1681 tgatgggcac ccgctcgggc gacatcgacc ccagcctggt gcagttcatc ggcaatgcgc
     1741 tgaatcaatc ggctgatcaa gtgattgacc tgctgaaccg caagagcggc ctgctgggcc
     1801 tctccggctt gtccaacgac tgccgcaccc tgcaagccgc cgccgaagaa ggccacgcgg
     1861 gcgcgcgggt ggcgctggag gtgttctgct accgcctggc caagcatatc gccggcctgt
     1921 ccgccgcgct gccgcgcctg gacgcgctgg tgttcaccgg cggcattggc gaaaactcgg
     1981 cctgggtgcg cgagcaggtg ctggcgcggc tgggcctgct gggctttgcc gtggatagcg
     2041 ccgccaacgc ccgctgtgtg ggcggcgtgg ccggcccgat tcacgccgcc ggcagccgca
     2101 ccgtcttggt ggtaaacacc aatgaagaac tgctgattgc ccgcgacacg tttgccctgg
     2161 caggcgtgtg acaccagact gaacaaggcc gcgcactgcg cggccttgtg cttttgtcct
     2221 gacgttctga ttttattctg atcatgatgc aacccgggtc gcgccacgcc tggtgggctt
     2281 gcctgtccag ccggcggccg gcgcaaccct cttccaacgg caaaggaaat tcatgcacgc
     2341 cctctacctc tccccggttg gctatgacgt cggcctgact tcggtggcgc tgggcctggt
     2401 gcgcgcgctt gagcgccgtg gcctgaaagt cggcttcatc aaaccggtgg cgcaacagcg
     2461 cgaccccggc gccgggcccg agcgctccag ccacttcgcc tccagcctgt tccgcctgga
     2521 caccccgccg ccaatcgact ttgcccgcgc cgaacaactg atgggcgccg gccagaccga
     2581 ccagttgctg gaagaaatcg tcagcctgta tcagcaagtg gtggaccaca atgatgtggt
     2641 ggtggtggaa ggcctggcgg ccgacgccaa ccatgtctac gcgccgcagc tcaataccca
     2701 gattgcccgc acgctgaacg cagaaaccat ttttgtcggc tctgctgccc gtctgtcggc
     2761 acaggaactg gccgaacaaa tcgaagtcgc cgcccgccag tcagcggaat cgcacaataa
     2821 ccgctttgcc ggctacatca tcaataaggt gcgcgaagac gtcgcgccgg gggattacac
     2881 cctggacatc gcccagctca ccgccaccgc caaggcgcgc cgcctgccgc tgctgggcac
     2941 cattccgctg tccagcaatt ttgccgcgcc gcgcacgctg gacatcgccc aacacctgaa
     3001 cgcccgggtg ctggcgccgg cacggctgca ttcggcacgg gtcagcgata tgctgattgc
     3061 cgcgcgctcg atcacccatt tgatcaaccg gctcaagccc ggcgcgctgg tgatcacccc
     3121 cggcgaccgc gacgatgtgg tgttggccac cgccatggcc gcccagcgcg gtgtgccgct
     3181 ggccggcctg ctgctgacct gtaattcgct gccgcacccg cacctgcagg cgctgtgcca
     3241 gatcaacgaa cacgccagct tcccggtgct ggccaccgaa ctggaaacct tcgacaccgc
     3301 ccgccagctc acgcagatgt cgcaccatgt gccggtggac gacctggcgc gcatggaacg
     3361 ggtggtggat tttgtcgccg agcggctgga cctggatcgg ctgatttcgc gcattggcca
     3421 gccgggcgaa gtacgactgt cgccgccggc cttccgctac cagctgattc aacacgcccg
     3481 ccgcgccggc aagcgcattg tgctgcccga gggcgacgag ccgcgcaccg tcgaggcggc
     3541 ggtgatctgc caccagaagg gcattgcccg ctgcgtgctg ctggccaaac cagaagccgt
     3601 acaaaaagtg gccgacgccc agggcatcac cctgccggcc gatctggaaa tcctcgaccc
     3661 cgaccacgcc cgcgcccgct acgtgccgcc gctggtcgag ctgcgccgcc acaagggcat
     3721 gaccgcgcac atggccgagc aatacctgga agacagcgtg gtgctgggca ccatgatgct
     3781 ggcggtgaac gacgtggacg gcctggtgtc cggcgcggta cacaccaccg ccaacaccat
     3841 ccgcccggcg ctgcaattca tcaagaccgc gccgggctcg agtattgtct ccagcgtgtt
     3901 cttcatgctg atgccggatc aggtgctggt gtacggtgac tgcgcggtga accctgaccc
     3961 cagcgccgaa gagctggccg acatcgccat ccaaagcgcc gactcggcca aggcctttgg
     4021 catcagcccg cgcgtggcga tgatttccta ttccaccggc agctccggcg ccggcagcga
     4081 tgtggaaaaa gtccgcgccg ccaccgagct ggtgcgccag cgccgcccgg acattctggt
     4141 ggacggcccg ctgcaatacg acgccgccag cgtggaaagc gtcggccggc aaaaagcccc
     4201 ggacagcccg gtggccggcc gcgccaatgt gtttgtcttc cccgacctca acaccggcaa
     4261 caccacctac aaggcggtgc agcgctcggc caatgtggtc agcgtcggcc cgatgctgca
     4321 aggcctgcgc aagccggtca acgacctgtc gcgcggcgcg ctggtggacg atattgtcta
     4381 caccattgcg ctgacggcga ttcaggccga gcaaatggcg aattaagccc gcgccagaca
     4441 cccggccggc acaccggccg ggcaaaaaac atcaggcccc acggtattca cctggggcct
     4501 gatgtttttg gcgcggtctg gcaggcttac ggcttgggtg cagccgccgc ttcgactgcc
     4561 gccgggtctg gcagattcac cgggcgatta ttgatggtga gcttgccgtt cttccattcg
     4621 gccgaggttt tcagctggcc gccttcttgt tgcagatagc cctgctcaga ccagatatcc
     4681 acctggctgg caaacaggtt ttttgccagc tcgtcgattt cttgcagcga cggcgggttt
     4741 tcggcgctgg cgtccaccgt gaacagattg cgcgcctgcg ccaccaccag gccttgcagg
     4801 gtgtcgcgcg gcacactcag cttggcgttg gcggtgagtt tgctcaggaa cacccgcgag
     4861 tcgttcaaat cacccttttg gtagccattc agcgccacat tgccggtgac ggtgacctcg
     4921 ccgctgggca gcttcaacga gaaatcgttg atcgccaggc gcgggttgtt ttccagaatc
     4981 gggccaccct cgcgcaccac ggcgtcaata tattgctgac gcagtttgtc cggctcgacg
     5041 ccttcaacct ggatgtccga gaacgcctgt tccagcttca acaggctggg gccgtgcagg
     5101 tggttgaccg acaaatccag ccgcatcggg ccgtaggcgg tgtggttgac ttccagtttt
     5161 tcaaagccaa acttggcgcg ggcgttgatg aattcttttt cttcgctgct gacgatctgg
     5221 tatttcaaat tcaccaaatc cgcctgcgaa ggcttgattt cgccagccgg attgatgaac
     5281 tcgccaatgc ggatgcgcgt cagcagcgag atcagctcgt tgagcttgat ctgatacggg
     5341 atgttttctt tcgattccag cttgacccga gtgaccgaca gctctttatt gcccacgccc
     5401 gcgccggtcg cccctgggcg ggtgtcagac tgatagctca gattgctgaa ttcgatatgg
     5461 cccttggtgg cggcatccag caccaggccg ggcaggcggg cgtcgatgct gtattggctg
     5521 taatccgagt cgtagcccac ctgggtggtc atgccctgcc atttgatctt gaccccggcc
     5581 agggtttctt cgtagtcaaa cgccggcacg ctgacgttca gctcgccgcc gccggtgaaa
     5641 ttcagccggt tggtgacggt gatcggctct ttgtcgccaa aaaagcgcga cagcatcttg
     5701 cgcgtgtcgg gcgacatgat gaattcagtg gtcaccaccg cgcgcgccgg gcgcaggccg
     5761 ccaggcagcg ggccatgctg aatgtggttg acgtatttta ccgtgccggt cagcgcattc
     5821 ttggccgcct cgggcagaat gccctgatac ggggcaaaaa agcgctcgtt gaaggtcagt
     5881 tcggtggttt cggtagacga aaaccagccg cgctcgtatt gatgcgactt gaccttgaaa
     5941 atcggcaggc cggcaatcat gtcgtgctgc ttggccagcg tgcgttccgc ttccagccca
     6001 gtccagtagg cgccggcgct gtacaacacg gcgataccca gcacacccgc cactgagccc
     6061 ttcaagagac gcgtcttcac cctgatgaat tcctgtatgc aaaacaatcc gcgcctatct
     6121 tagtccattt cctggccggg ctgtactgtt ggatatacct gtacccggcc ttggcgacaa
     6181 aaaaccggcg gcacagacgc cgccggcggg atcagtcagc gggatgc
//
LOCUS       DAOPYX010000180         6203 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_15600_length_6203_cov_7.894925, whole genome shotgun sequence.
ACCESSION   DAOPYX010000180 DAOPYX010000000
VERSION     DAOPYX010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 6203)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6203)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6203
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15600_length_6203_cov_7.894925"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..772
                     /gene="fliD"
                     /locus_tag="PKN55_09590"
     CDS             <1..772
                     /gene="fliD"
                     /locus_tag="PKN55_09590"
                     /inference="COORDINATES: protein motif:HMM:NF018852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HNX71538.1"
                     /translation="TSTIEVTSVPIKAKLEGGAVAPTSFPAYITGANVLKLSIDGTPV
                     TANLATSYVSAAAMAAGMQTAINTSLPAARQVTVAYKAGKFEITSGKTGNTSKVEVLS
                     GTTLSPSFGLTVGSTASGTALAGKIGGVAALADDDSMTLIGATGSGASGLKIRVDTPT
                     LGSRGSVSFARGFAYNMGDTLSRVLSLNGPIETRKSSLNSDISRIDDQVDRLNDKLVR
                     METTLRKQFTAMDSTVGKYNQLSTYLASQLKSLSGSSS"
     gene            806..1294
                     /gene="fliS"
                     /locus_tag="PKN55_09595"
     CDS             806..1294
                     /gene="fliS"
                     /locus_tag="PKN55_09595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018747997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HNX71539.1"
                     /translation="MSTKRNPYEAYSKTSLESEVAQASPHKLILMLFDGLLSAIKQAK
                     THMEAKRIAEKGMALSKAVAILEEGLRSSLDKEVGGELAENLDALYEYCSDRLIEANL
                     KNKPELLDEVVKLLTPIRDAWETIGQPGYLQGEAPQPAAQPAAEATAPENRNSPLSYG
                     HV"
     gene            1287..1616
                     /locus_tag="PKN55_09600"
     CDS             1287..1616
                     /locus_tag="PKN55_09600"
                     /inference="COORDINATES: protein motif:HMM:NF017237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FliT"
                     /protein_id="HNX71540.1"
                     /translation="MSEPDQGTFSDWLKVLTLAQEAHQAAAQANWERFLELEDQYVQA
                     LLATQQQPVEIANLDEARRAAFTELVKHVIALHQETVQWAEAHRNALATELGMVTQHS
                     KVLKAYG"
     gene            1786..2184
                     /locus_tag="PKN55_09605"
     CDS             1786..2184
                     /locus_tag="PKN55_09605"
                     /inference="COORDINATES: protein motif:HMM:NF022428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2802 domain-containing protein"
                     /protein_id="HNX71541.1"
                     /translation="MDPLIPWLSLGLAVAALAGMGAMFWYMRRQQASQAREIEQTYFD
                     LQQSQLSALQRDVKALLARLEKLEQVATTNHASSALTPTGAAVPGTTPYNQAIELARQ
                     GMPAAEVATRCGISRSEAELIVALYRRARS"
     gene            2505..3632
                     /locus_tag="PKN55_09610"
     CDS             2505..3632
                     /locus_tag="PKN55_09610"
                     /inference="COORDINATES: protein motif:HMM:NF014207.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HNX71542.1"
                     /translation="MPDSSSKVLLSPATRYLSTLLFVANAADDAAQPKNLPLIFQNKP
                     GIGIASVSSLVAELADSVKSAAVFGGAKLSTVSESATAMVKTKMALDPELSKGTTQAQ
                     SSQNVPRVLDAAGKAQSASAARFPPGLSSLLGGEKANAGGQALALSPLFENMDALPTT
                     GMAQLLKQAVTRSGVFYESHLADWAQSGRSLHALRQEPQANLKLPEKLAELGKPDGQN
                     NASVQEAMKTGSATGLRDRELAGLRNDSNTTQLGQLVHKQLDALDQNQFSWQGIAWPG
                     QAMDWRIEREQVDQREAHGGGEEESRWSTSLSMELPNLGGLAAKIALTPAGVIVRFQT
                     DSADTAQAIEDNQLRLRENLQRAGLSLLNFAVSQGEQPNAG"
     gene            3622..4023
                     /locus_tag="PKN55_09615"
     CDS             3622..4023
                     /locus_tag="PKN55_09615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018747992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HNX71543.1"
                     /translation="MQAKPNRSAVALAYKAGHAAPHVVAKGRGLLAETIIEKAKEAGL
                     FVHESPELVSLLMQVDLDKSIPAELYQAVAEILAFVYYLERQAKGSSQNVDDLLAQAG
                     QALTLPLPVALPAAAPAAADSATPATPPTPR"
     gene            complement(4049..5344)
                     /locus_tag="PKN55_09620"
     CDS             complement(4049..5344)
                     /locus_tag="PKN55_09620"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008953479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HNX71544.1"
                     /translation="MGLLDFAHQDYTPSWYADSAPRLAPQPALRGEVSAEVCVIGGGY
                     TGLSTALELAERGVSVVLLEGARLGWAASGRNGGQVLSDLACGMDAVRAELGAEAARA
                     IWAMTQEAARMVGERCQRYAIDADLHWGYLHAAVKPRQLRELADWQARAARDYGYEAL
                     RLVEQAGLSALVDSPRYVGGLFDPNCGHLHPLKYALGLARAALAAGVRVYEHSPALRI
                     EPGASVTVVSEQGRVRAEQVVLAGNCHLGGLAPTLRARIMPVGTYIIATAPLGEARAR
                     QLLPGDDAVSDCHFVLDYFRRSADHRLLFGGKVSYSGLPPANLKAAMRRDMLRVFPQL
                     ADVAIDSGWGGFVDITRSRAPDFGQLADNIWFAQGFSGHGVALTGLAGRLIAEAIVGQ
                     PQRLAWFQRFRHARFPGGRWLRTPALVLGMAYYRLRDWL"
     gene            complement(5360..6193)
                     /locus_tag="PKN55_09625"
     CDS             complement(5360..6193)
                     /locus_tag="PKN55_09625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014085886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNX71545.1"
                     /translation="MHSTSSMSWFGRLSLGGGLAFLYLPIVVLVVYSFNASKLVSVWA
                     GFSLGWYQTLFDDEAVMAAFGLSLKVAVLASAMSVVLGTLAGLVMARFGPFRGKSLFA
                     GMLTAPMVMPEVITGLSMLLLFVSMQQWVGFPEERGVLTIWVGHVTLCTAYVTVVIRS
                     RLVEMDRSLEEAAMDLGARPLKIFFVITLPLILPAIASGFLLSFTLSLDDLVMTAFLS
                     GPGSTTLPQLIFSRVRLGLNPEINALGTLTVLVVAVLVISANRIMLRQEKRRAALAAA
                     S"
BASE COUNT         1368 a         1941 c         1785 g         1109 t
ORIGIN      
        1 cacgtctacg attgaggtta ccagtgtacc aatcaaggcc aagctggagg gaggagcggt
       61 ggctccaact tcttttccag cctacattac cggcgccaat gtactgaagc tgagcattga
      121 tggtacgccg gtcacggcta atctggcgac ttcctatgtc tctgctgcgg caatggcagc
      181 tggtatgcag accgctatca atactagttt gcctgctgcc cgccaagtga ctgtcgcgta
      241 caaagccggt aagtttgaaa tcacctcggg caaaacgggc aatacctcca aggtggaagt
      301 gctctcgggc actacgttgt ctcccagttt tggcttgacg gtgggctcaa ccgcttctgg
      361 cactgctctg gctggtaaga ttggcggtgt tgctgcattg gctgacgatg acagcatgac
      421 actgatcggt gcaacaggaa gcggcgcttc tggcttgaag attcgagtcg atactccgac
      481 attaggctcg cgtggttcgg tttcctttgc gcgaggcttc gcatacaata tgggggatac
      541 actgtcccga gtcctgtcgc tgaatggtcc gatcgaaacg cgcaaaagtt cgttgaacag
      601 tgatatttcg cgtatcgatg atcaggttga tcgcttgaac gataagctgg tgcgtatgga
      661 aactactctg cgcaaacaat ttacggcgat ggactcaacc gttggcaagt acaatcagct
      721 gtcgacctac ttggcttccc agttgaaatc gctgtccgga tcatcaagct aagctcgcag
      781 cttttatcat tatccaggaa ctggtatgtc cacgaaacgc aacccttatg aagcctatag
      841 caagacctct ctggagtctg aggtcgccca ggccagccca cacaagctga tcctgatgct
      901 gtttgatggc ctgcttagtg ccatcaaaca ggcgaaaacc catatggaag ccaagcgcat
      961 tgccgaaaaa ggtatggcgc tgagtaaggc agtcgccatt ttggaagaag ggttgcgcag
     1021 ttccttggat aaagaagtcg gcggtgagct tgccgaaaac ctcgatgcgc tctatgagta
     1081 ctgctcagat cgcctgatcg aagctaatct gaaaaacaag cctgaactgc tggatgaagt
     1141 ggtcaagctg ctgaccccga ttcgcgacgc gtgggaaacc atcggccaac caggctactt
     1201 gcaaggcgaa gcgccacagc ctgctgctca gcctgccgca gaggcgacgg cgccggaaaa
     1261 ccgcaattct ccgttgagct acggccatgt ctgagcctga tcaaggtacg ttcagcgatt
     1321 ggttaaaagt tctcacgctg gcccaggaag cccatcaagc agcagcgcag gcaaactggg
     1381 aacgttttct ggaactggaa gaccagtatg tacaagcttt gctggctacc cagcaacagc
     1441 ctgtcgagat tgccaatctg gacgaagctc gccgtgcagc attcactgag ctggttaagc
     1501 acgttatcgc actccatcag gagaccgtgc aatgggccga agcgcatcgt aatgcgctcg
     1561 cgactgagtt aggcatggtg acccagcaca gcaaagtgct caaggcgtat ggttgattgg
     1621 tcagatattc caatgtgttg gccgggcgtg gcaaactctt gagtccggcc atggagtatc
     1681 ttcacgtgat gaaatggctt tgcactcgca aggccatttt ttcttggtgc atcgggcaaa
     1741 accactgcca gatgatattg caaataaaaa caggtgcata cgacaatgga tccgttgatt
     1801 ccgtggcttt ccctgggact ggctgtggcg gcgctggcag ggatgggggc gatgttctgg
     1861 tatatgcgcc ggcagcaggc cagtcaggcg cgggaaatcg agcaaaccta cttcgatttg
     1921 cagcaaagtc agctcagcgc attacagcgt gacgtcaagg ccttattggc gcgcttagag
     1981 aaactcgagc aggttgctac aaccaaccat gctagctcgg cgctcacgcc cacgggagca
     2041 gctgtgcctg gcacgacacc atataatcag gccatcgagc tggcccgcca aggcatgccc
     2101 gctgctgaag tagccactcg ctgcgggatt tcccggagcg aggccgaact gattgtggcc
     2161 ctgtatcggc gggccagatc atgatcccgg tttcttcttc cctccagccc aatcccgagc
     2221 aactggcacg tttgctgcgg gaaagcggca aacccaccct tgaactttcc agcgccgcag
     2281 ccaaggcgct gccctctttt gaggtcggcg aggaagtcac cgccaagatt gttgacactt
     2341 acggcagcaa ccgtttcacc gtgctggtca aagacaaact gatgaccttg aacctggatc
     2401 gtcgccatgc ttttgtcggc gactccggca gtaccatcaa gctggtggtc gccacggttg
     2461 agcccaagct gtcgtttgcc atggacgcca aggcgcccat gcacatgccg gattcgtcca
     2521 gcaaggtctt gctcagcccg gccactcgct atttatctac actgttattt gttgcaaatg
     2581 ctgctgatga cgcagcccaa cccaaaaatt tgccattaat tttccaaaat aagccaggca
     2641 ttggcattgc ctcagtcagt tctttggtcg ccgaactggc tgacagcgtt aaatctgctg
     2701 ctgtgtttgg aggcgcaaag ctatccactg tgtctgagtc tgctactgcg atggtcaaaa
     2761 ccaaaatggc attagaccct gaactcagta agggcactac tcaagctcaa tcaagtcaaa
     2821 atgtgcctcg tgttttagac gcggccggca aggcacaaag cgccagcgcc gcgcgttttc
     2881 cgccgggttt atcatcgttg ttgggtggcg agaaagccaa tgcgggtggc caggctctgg
     2941 cgctgagccc cttgtttgaa aacatggacg cgctgcccac taccggcatg gcacaattgc
     3001 tcaagcaagc ggtgacgcgc agtggtgtgt tttatgaatc gcatctggct gattgggcac
     3061 aaagcgggcg cagcctgcat gccttgcgcc aagagccaca agccaacctc aagctgccgg
     3121 aaaaactggc tgaactgggc aagccagacg ggcagaacaa tgcctcggtg caagaagcca
     3181 tgaaaaccgg ctcggccacc ggtttgcgcg accgtgagct ggcgggcctg cgcaatgaca
     3241 gcaacaccac gcagttgggg caattggtgc acaagcaact ggatgcgctg gaccaaaacc
     3301 aattcagctg gcaaggcatt gcctggcctg ggcaagccat ggattggcgc atcgaacgag
     3361 aacaagttga tcagcgcgag gcccatggcg gcggtgaaga agaaagccgc tggagcacca
     3421 gcctgtcgat ggaactgccc aatctgggcg ggctggccgc caagattgcg ctgacgcccg
     3481 ccggggtgat tgtgcgcttt cagaccgatt cagccgatac cgcacaagcc attgaagaca
     3541 atcaactgcg cctgcgcgaa aatctgcaac gggccggcct gtccctgctg aattttgccg
     3601 tgagccaggg agagcagccc aatgcaggct aaacccaacc gcagcgccgt ggcgctggcc
     3661 tacaaagcgg gtcatgccgc gccgcatgtc gtcgccaagg ggcgtggcct gctggcagaa
     3721 accattattg aaaaagccaa ggaagccggc ctgtttgtac acgaatcgcc ggaactggtg
     3781 tccttgctga tgcaagtgga cctggacaag agcattccgg ccgagctgta ccaggcagtg
     3841 gccgagatcc tggcatttgt gtattacctg gagcgccagg ccaagggcag cagccagaat
     3901 gtggacgatc tgctggccca ggcgggccag gcgttaacct tgcctctgcc ggtggcgctg
     3961 cctgccgccg cgcctgctgc tgcggattct gccaccccgg cgactccccc cacgccgcgc
     4021 tgagtgcgca cacaccatca cgccgcgctc acagccagtc gcgcagccgg taatacgcca
     4081 tgcccagcac cagggccggc gtgcgcagcc agcgcccacc aggaaaacgg gcgtggcgaa
     4141 agcgctgaaa ccaggccagc cgctgcggct ggccgacaat cgcctcggca atcagccgcc
     4201 cggccaggcc ggtcagcgcc acgccatggc ccgaaaaacc ctgagcaaac cagatattgt
     4261 ccgccaactg gccaaaatcc ggcgcccggc tgcgggtgat gtccacaaaa ccgccccagc
     4321 cgctgtcaat cgccacgtcc gccagctgcg gaaacaccct gagcatatcg cggcgcatgg
     4381 cggctttcag attggccggc ggcaggccgg aataactgac cttgccgcca aacagcagcc
     4441 gatggtcggc cgagcggcgg aaataatcca gcacaaaatg acaatctgac accgcatcgt
     4501 cgcccggcaa gagctggcgc gcgcgcgcct cgcccagcgg cgcggtggcg atgatatagg
     4561 tgcccaccgg catgatgcgc gcgcgcagcg ttggcgccag cccgcccaga tggcaattgc
     4621 cggccagcac cacctgctcg gcacgcaccc ggccctgttc gctcaccacc gtcaccgacg
     4681 cccccggctc gatgcgcagc gccgggctgt gctcatacac gcgcaccccg gcggccagcg
     4741 ccgcgcgggc caggcccagc gcgtatttca gcggatgcag atgaccgcaa ttcgggtcga
     4801 acagcccgcc gacatagcgc ggcgaatcca ccagcgcaga caaacccgcc tgctcgacca
     4861 gccgcagcgc ctcgtagcca tagtcgcgcg cggcgcgcgc ctgccagtcg gccagctcgc
     4921 gcaactggcg cggcttgacc gccgcgtgca aataccccca gtgcaaatcg gcgtcgatgg
     4981 cgtagcgctg gcagcgctcg cccaccatgc gcgccgcttc ttgcgtcatc gcccagatgg
     5041 cgcgcgccgc ctcggcgccc agctcggcgc gcaccgcgtc catgccgcag gccagatccg
     5101 acaacacctg gccgccattg cgcccggacg ccgcccagcc cagccgcgcg ccttccagca
     5161 acaccacgct gaccccgcgc tcggccaact ccagcgcggt ggacagcccg gtgtagccgc
     5221 cgccaatcac gcatacctcg gcgctcacct caccgcgcaa agccggctgc ggggccaggc
     5281 gcggcgcgct gtcggcatac caactgggcg tgtagtcctg atgggcaaaa tccagcaatc
     5341 ccatggccga ctcccccgtt cagctggccg ccgccagcgc ggcgcggcgt ttttcctggc
     5401 gcagcatgat gcggttggcg ctgatcacca gcaccgccac caccagcacg gtcaaggtgc
     5461 ccagcgcgtt gatttccggg ttcaggccca gccgcacccg cgagaaaatc aactgcggca
     5521 acgtggtcga gcccggcccg gacagaaacg ccgtcatcac cagatcgtcc agcgacaggg
     5581 tgaatgacag caaaaagccc gaggcaatcg ccggcagaat caagggcagg gtgatgacaa
     5641 aaaagatttt cagcggccgc gcgcccagat ccatcgccgc ttcttccagt gaacggtcca
     5701 tctccaccag ccgcgaacgg atcaccacgg tgacataggc agtgcacagg gtgacatggc
     5761 ccacccagat ggtcagcacc ccgcgctcct ccggaaagcc cacccactgc tgcatgctga
     5821 caaacagcag cagcatggac aggccggtga tgacttccgg catcaccatc ggcgcggtca
     5881 gcatgccggc aaacaggctc ttgccgcgaa acgggccaaa gcgcgccatc accaaaccgg
     5941 ccagcgtgcc cagcaccacg ctcatggcgc tggccagcac ggcgactttc aggctcagac
     6001 caaacgccgc catcaccgcc tcgtcgtcga acagggtttg ataccagccc agcgaaaacc
     6061 cggcccagac gctgaccagc ttggacgcat tgaatgaata caccaccagc accacgatgg
     6121 gcaggtagag aaaagccagc ccgccgccca gcgacaagcg gccaaaccaa gacatcgaag
     6181 atgtggaatg catgattccc tcc
//
LOCUS       DAOPYX010000181         6150 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_15804_length_6150_cov_8.227400, whole genome shotgun sequence.
ACCESSION   DAOPYX010000181 DAOPYX010000000
VERSION     DAOPYX010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 6150)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6150)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6150
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15804_length_6150_cov_8.227400"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            21..179
                     /locus_tag="PKN55_09630"
     CDS             21..179
                     /locus_tag="PKN55_09630"
                     /inference="COORDINATES: protein motif:HMM:NF036629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5397 family protein"
                     /protein_id="HNX71546.1"
                     /translation="MKTFGPFGPKYAVGVPLRQLEDGDWLIEITMVETGEKTEYRLAH
                     LLDDPEAR"
     gene            179..457
                     /locus_tag="PKN55_09635"
     CDS             179..457
                     /locus_tag="PKN55_09635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor"
                     /protein_id="HNX71547.1"
                     /translation="MYAVAFDLVVADTEQHHPKGVAQAYTEIGAILAEFGFRRVQGSL
                     YVSDSEDMAQLFLAIQALRARAWFPKSVRDIRAFRIEQWSDFTAVVKA"
     gene            complement(675..1541)
                     /locus_tag="PKN55_09640"
     CDS             complement(675..1541)
                     /locus_tag="PKN55_09640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX71548.1"
                     /translation="MNLSSLTDSLRSRFANLNPLNRPVAQAATVSEAKTASTSTPDTK
                     VQLGGRDSGPSIYDRFGQLSSNDAASARQRANEKVAELKKRMKLLKSMMAGASPAQRK
                     ALARQLGQIANELKAAVKNFSDASGSGQTSAPKSANAAISPDNTAQQANPQTATAGST
                     PDSTGANSTETGKHQANAQTAAASGQSGQGASNKEKQDFVREAKELASDIKRTLEMLK
                     RKLRRQSDSVVRQAEKALGDIGKMLNNLSGGATQSSAGSGDASLNIQVAAVSPASNPG
                     SVSISAGSVTAG"
     gene            1753..2610
                     /locus_tag="PKN55_09645"
     CDS             1753..2610
                     /locus_tag="PKN55_09645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019921943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HNX71549.1"
                     /translation="MNPPTHLPIMAAFAALISVQTGAAFAKSLFPLVGAEGVAALRLG
                     LSALWLSLWFRPWRLWRARPDWLALLGYGIMLGLMNLLIYRAFMYIHVSLAVSIEVIG
                     PLAVALLNSRQRRDVLWVGLALLGLALLPFGQLGSGLDVRGVAYALAAALCWALYVVF
                     GARAASGGGQAVATGMLVAALFAVPLGVAQSGAQLLTPYALAIGLVVAVLSSMLPFLL
                     DMYAMRWLPARVFGVLLSASPAMSALAGWWVLGETLGPLQILGVSAIMLACAGSALFA
                     RPASTGDQS"
     gene            2617..3510
                     /locus_tag="PKN55_09650"
     CDS             2617..3510
                     /locus_tag="PKN55_09650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019924916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-binding protein"
                     /protein_id="HNX71550.1"
                     /translation="MHMVLKYCKQLGVGLVLSALAATAGAQVQVRDDAGQMVTLNAPA
                     RRVISLAPHVTELIYAAGGGARLVGVVSHSDYPEAAKQVPRVGDNRQVDIERVLALKP
                     DLLVVWLHGNAERQLEPLRRLGVPLFYSEPTKLEAIPETLLKLGTLMGSEPQAQAAAG
                     QFRQRLAGLRQHYAQARPLRVFYQVWDQPLYTLNGQHMVSDVLRLCGGVNIFADLPVT
                     APTVTQEAVIAADPDVILAGEMSGRGAVDSWKRFPRMRAVRQQQLYTLNGDLLHRPGP
                     RILDGASQVCQRLAQARAQRP"
     gene            complement(3518..4588)
                     /gene="corA"
                     /locus_tag="PKN55_09655"
     CDS             complement(3518..4588)
                     /gene="corA"
                     /locus_tag="PKN55_09655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008953079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HNX71551.1"
                     /translation="MSRHPSARLSRHAGHAPGELVHIGETKTAEPRILLIEFSPEHYQ
                     ETTFASLAASRTHQPGDGTLWLNVYGLQDVAMMAEIGRRFQLHPLVMEDVLNTHQRPK
                     IEDYGDYIFIVTHVFDYQPASRTLSEEQISMVLGRNVVLTFQERPAGRFQPLRERLKN
                     GIGQGRRLGAGYLAYSLLDVIVDQYFVTLDQLGDTVEELDTRLLAGQADGGTLARIHT
                     LKMALLSLRRALWPLREAVMTLQHGDSALLDDETRVYLRDVYDHLVHLLETVDMQRDL
                     IAGMVDLYISTQSNQMNRQMRFLTVIATLFMPLTLISSIYGMNFEYMPELHWKWGYFI
                     VLGVMAAIAAAMGYLFKRWRWW"
     gene            complement(4585..6078)
                     /gene="ppx"
                     /locus_tag="PKN55_09660"
     CDS             complement(4585..6078)
                     /gene="ppx"
                     /locus_tag="PKN55_09660"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009118088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004309 - exopolyphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006798 - polyphosphate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HNX71552.1"
                     /translation="MPSSFETLAAVDLGSNSFRLQIVRVDGEQFFPLDTLKDTVRLGA
                     GLTADNFLDDATQERALTCLARFGDRLRHFRPDQVRVVGTNTLRVAKNSAAFIREAEA
                     RLGFPIEIIAGREEARLIYVGAAHSLPAGKEKRLVVDIGGGSTEFIIGSGMKAQKTES
                     LGLGCVTYSLRYFPDGKLSKANFKNAEVAARNEIQRIAHDYTPDQWQLAVGTSGTARS
                     LRDILELNDYSVDGITRAGMERLKTELLRCGSMEAVNLNGLRADRAPVLPGGLAIMLA
                     IFEELGIEQMTVTLGALRDGVLYDLLGRQYHKDLRDVTVTQFKRRYHVDTGQTERVAG
                     LARQFYAQLAGSEAMQHPEIPRQLEWAAKLHEIGLMISHSSYHKHSAYIVEQADMPGF
                     SRREQRQIAHLILAHKGSLSKMVALLNEQPAYWPAVLALRLAVLFSRSRRPVELPTTL
                     QLSAAPRGFTLTVDGDWLASTPLTAGALKQEEAPWRATGLSFHVRQA"
BASE COUNT         1001 a         1805 c         2073 g         1271 t
ORIGIN      
        1 ccccacctca ccccggtgaa atcaaaacct tcggcccatt tggccctaaa tacgccgtgg
       61 gggttcctct tcgacagctg gaagatggcg actggttgat tgaaatcacc atggtggaaa
      121 ccggtgaaaa aacggaatac cggctggccc atctgctgga cgacccggag gcgcgctaat
      181 gtatgccgtg gctttcgatc tggtggtggc tgacaccgaa caacaccacc ccaaaggcgt
      241 cgcccaagcc tataccgaaa taggcgcaat tctggcggag ttcggttttc gccgggtgca
      301 gggcagtttg tatgtgtccg acagcgaaga catggcgcag ttgtttctgg ccattcaggc
      361 cttgcgtgct cgcgcctggt ttcccaagtc ggtgcgcgat attcgcgcct tccgcattga
      421 gcagtggtcg gatttcaccg ccgtggtaaa ggcctgattt tgccaggctt ggccagccaa
      481 gcacccataa aaaaatagct gaattcgcat cagcatggga cagggtcacg cccggcgggt
      541 ggctcagtgc gataccgcga ccgattgaac ctggccaccc agggcacccg gcaattcatg
      601 cgaaacgaat gcggcgaccg ctccttgtac gggccaagag acagcggcaa accggtagcg
      661 atgcccaaca accatcagcc agccgtcaca ctgcccgcac tgatactgac actccccgga
      721 ttgctggccg ggctgacggc ggcgacttga atattgagcg aggcatcgcc gctgccggcg
      781 ctgctctgtg tggcgccacc gctcaggtta ttcagcatct tgccgatgtc gcccagggct
      841 ttttcagcct ggcgcaccac gctatcgctc tgccggcgca gtttgcgctt gagcatctcc
      901 agggtgcgtt tgatgtcgct ggccagttct ttggcctcac gaacaaagtc ctgcttttct
      961 ttattgctgg cgccttggcc actctggccg ctggcggcgg ctgtctgggc gttggcttgg
     1021 tgcttgccgg tttccgtact gttggcgccg gtgctgtcag gtgtgctgcc ggcggtggcg
     1081 gtttgcgggt tggcttgctg agcggtgttg tctgggctga tggccgcgtt ggcggatttc
     1141 ggggcgctgg tttgcccgct gccgctggcg tcggaaaaat tcttcaccgc ggctttcagt
     1201 tcgttggcaa tctgccccag ttggcgcgcc agagccttgc gctgtgccgg gctggcgccg
     1261 gccatcatgg atttcagcag tttcatgcgt tttttcagct cggccacttt ttcgttggcg
     1321 cgctgacggg cgctggcagc gtcattgctg ctcagctggc caaagcggtc ataaatgctt
     1381 gggccgctgt cacggccgcc caattgcact ttggtgtctg gcgtgctggt cgatgcggtc
     1441 ttggcttcgc tgacggtggc tgcttgggcc acgggacggt taagcgggtt caagttggca
     1501 aagcggctgc gcaaggagtc ggtcaggctg gacaggttca tggctggctt tcgttgagcg
     1561 tcaggttttg ttatcgtctg tcattggcgc aggttaagcg cgccatcgac caaacttctg
     1621 gcatgaatgc gcatttcagc catctggcgc gataaaccac gccagaaatg ttgattcacg
     1681 caataaattt atcccttgcg gctggcgctg catgctgctt gtcctgctga ttgcttaccc
     1741 cgaacgctgt gcatgaaccc tcccacgcat cttccgatta tggcggcatt tgccgcgctg
     1801 atttccgtgc agactggcgc ggcatttgcc aagtcgctgt ttccgctggt gggcgccgag
     1861 ggggtggcgg ccttgcgcct gggcctgtcg gcgttgtggc tgtcgttgtg gttccggccg
     1921 tggcgcttgt ggcgggcgcg gccggactgg ctggcgctgc tgggctacgg catcatgctg
     1981 ggcttgatga atctgctgat ttatcgggcg tttatgtata ttcacgtcag cctggccgtg
     2041 tcgattgagg tgattggccc gctggcggtg gccttgctga attcgcgtca gcgcagggat
     2101 gtgctgtggg tgggcctggc gctgctgggc ctggccttgc tgccgtttgg ccagttgggc
     2161 agcgggctgg atgttcgggg cgtggcgtat gcgctggcgg cggccttgtg ctgggcgctg
     2221 tatgtggtgt ttggcgcgcg cgccgccagc ggcggcggcc aggccgtggc caccggcatg
     2281 ctggtggcgg cgctgtttgc cgtgccgctg ggcgtggcgc aatctggcgc acagctgctg
     2341 accccctacg cgctggccat tggcctggtg gtggcggtgt tgtccagcat gttgccgttt
     2401 ttgctggata tgtacgccat gcgctggctg ccggcgcggg tgtttggcgt gttgctcagt
     2461 gcgtcgccgg cgatgagcgc gctggcgggc tggtgggtgc tgggcgaaac gctgggcccg
     2521 ctgcaaattc tgggggtgag cgccatcatg ctggcctgcg ccggcagcgc gcttttcgcc
     2581 cggccggcga gtaccggcga tcaatcatga acaaggatgc acatggtgtt gaagtattgt
     2641 aagcagctgg gggtgggcct ggtgttgtcg gcgctggcgg ccaccgccgg ggctcaggtg
     2701 caggtgcgcg acgatgccgg gcagatggtg acgctcaacg cgccggcacg ccgggtaatc
     2761 agcctggcgc cgcatgtcac cgagctgatt tacgcagccg gtggcggtgc gcggctggtg
     2821 ggcgtggtca gccacagtga ttaccccgag gcggccaagc aggtgccgcg cgtgggggac
     2881 aatcgccagg tggatatcga gcgggtgctg gccttgaagc cggatttgct ggtggtgtgg
     2941 ctgcacggca acgccgagcg ccagctggag cctttgcgcc ggctgggtgt gccgctgttt
     3001 tacagcgagc cgaccaagct ggaggctatt ccggaaactt tgctgaaact gggcacgctg
     3061 atgggcagcg agccgcaggc gcaggcagcg gctgggcagt ttcgccagcg gctggcgggc
     3121 ttgcgccagc actatgccca ggcgcggccg ctgcgggtgt tttatcaggt gtgggatcag
     3181 cccttgtata cgctgaatgg tcagcatatg gtcagcgatg tgctgcggct gtgcggtggg
     3241 gtgaatatct ttgccgattt gccggtgaca gcgccaacgg tgacacaaga ggcggtgatt
     3301 gcggccgatc ccgatgtgat tctggcgggc gagatgagcg gacgcggcgc ggtggacagt
     3361 tggaagcgct ttccgcggat gcgcgcggtg cgccagcagc agctttacac gctgaatggc
     3421 gatttgctgc atcgcccggg gccgcgcatt ctggatggcg ccagccaggt gtgccagcgg
     3481 ctggcacagg ccagggcaca gcggccgtag caacggctca ccaccagcgc cagcgcttga
     3541 acagatagcc catggcggcg gcaatcgccg ccatcacgcc cagcacaata aaatagcccc
     3601 acttccagtg cagctcgggc atgtattcaa agttcatgcc gtagatgctg gaaatcagcg
     3661 tcagcggcat gaacagcgtg gcgatcaccg tcaaaaaacg catctgccgg ttcatctggt
     3721 tggattgggt ggagatatac aaatccacca tgccggcgat caggtcgcgc tgcatgtcca
     3781 cggtttccag caggtgcacc aagtggtcgt agacatcgcg cagatacacg cgggtttcgt
     3841 cgtccagcag cgcagaatcg ccatgctgca aggtcatcac cgcctcgcgc agcggccata
     3901 gcgcgcggcg cagcgacagc agggccattt tcagggtgtg gatgcgcgcc agcgtgccgc
     3961 cgtccgcctg gccggccagc aagcgggtgt ctaattcttc tacggtgtcg cccagctgat
     4021 ccagggtgac aaaatactga tcgacaatca cgtccagcag gctgtaggcc agataaccgg
     4081 cccccagtcg gcggccctgg ccaatgccgt ttttcagccg ctcgcgcagc ggctgaaagc
     4141 gcccggccgg gcgctcttga aaggtcagca ccacattgcg gcccagcacc atgctgattt
     4201 gttcttcgct cagcgtgcgg ctggccggct gatagtcaaa cacatgggtg acgataaaaa
     4261 tatagtcgcc gtagtcttcg attttgggcc gctgatgggt gttgagcacg tcttccatca
     4321 ccagcgggtg cagctgaaaa cgccggccga tttccgccat catcgccaca tcttgcaggc
     4381 cgtagacgtt cagccacagc gtgccgtcgc ctggctggtg ggtgcggctg gcggccaggc
     4441 tggcaaaggt ggtttcctgg tagtgctcgg ggctgaattc aatcagcaga atgcgcggtt
     4501 cggcggtttt ggtttcgcca atatgcacca gttcgcccgg cgcgtggcct gcgtggcggc
     4561 tcagccgggc ggacgggtgg cggctcatgc ctgacggacg tgaaagctca ggccagtggc
     4621 gcgccagggg gcttcttcct gcttcagcgc cccggcggtc agcggcgtgc tggccagcca
     4681 gtcgccgtcc acggtcaggg taaagccgcg cggcgcggcg ctcagttgca gagtggtcgg
     4741 caattccacc ggccgccggc tacggctgaa caacaccgcc aggcgcaggg ccagcaccgc
     4801 cggccagtag gccggctgct cgttcagcag cgccaccatc ttgctcagcg agcctttgtg
     4861 cgccaggatc agatgggcga tctggcgttg ctcacggcgc gagaagcccg gcatatccgc
     4921 ctgttcgacg atataggccg aatgcttgtg atagctggag tgcgaaatca tcaggccgat
     4981 ttcatgcagc ttggccgccc attccagctg acgcgggatt tccgggtgct gcatcgcctc
     5041 gctgccggcc agctgggcgt aaaactgccg cgccagcccg gccacccgct cggtctggcc
     5101 ggtgtccaca tggtagcggc gcttgaactg ggtgacggtg acgtcgcgca aatccttgtg
     5161 atactggcgg ccgagcaaat cgtacagcac gccatcgcgc agcgcgccca gggtgacggt
     5221 catctgctcg atgcccagct cttcaaaaat cgccagcatg atggccagcc cgcccggcag
     5281 cactggcgcg cggtcggcgc gcaggccgtt caggttcacc gcctccatgc tgccgcagcg
     5341 caacagctcg gttttcaggc gctccatgcc ggcgcgggtg atgccgtcca cgctgtagtc
     5401 gttcagctcc aggatgtcgc gcaaagagcg cgccgtgccc gaggtgccca ccgccagctg
     5461 ccattggtcg ggggtgtagt catgggcgat gcgctggatt tcattgcgcg ccgccacttc
     5521 ggcgttcttg aaattggcct tgctgagctt gccatcggga aaataccgca ggctgtaggt
     5581 gacgcacccc aggcccaggc tttcggtttt ttgcgccttc atgccgctgc cgatgatgaa
     5641 ctcggtcgag ccgccgccga tatccaccac caggcgtttt tctttgccgg ccggcaggct
     5701 gtgcgcggcg ccgacataaa tcagccgggc ttcttcgcgc ccggcgatga tttcaatcgg
     5761 aaatcccaga cgcgcctctg cttcgcggat gaaggcggcg gagttttttg ccacccgcag
     5821 ggtgttggtg cccaccaccc gcacctggtc ggggcggaag tggcgcaaac ggtcgccaaa
     5881 ccgcgccagg caggtcagcg cgcgctcttg ggtggcgtcg tccagaaaat tgtcagcagt
     5941 caggccagcg cccaggcgca cggtgtcttt gagggtgtcg agcggaaaaa actgctcgcc
     6001 atcgacgcgg acgatttgca gacgaaagct attggagccg agatcaacgg cggcaagggt
     6061 ttcaaacgag gaaggcacca gaaaacctcg ggacaggatg ggtaagagtc agttgggcga
     6121 tatgatagcg ggaaaggggc gcagatcaaa
//
LOCUS       DAOPYX010000182         6109 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_15950_length_6109_cov_10.978196, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000182 DAOPYX010000000
VERSION     DAOPYX010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 6109)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6109)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6109
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15950_length_6109_cov_10.978196"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            64..456
                     /locus_tag="PKN55_09665"
     CDS             64..456
                     /locus_tag="PKN55_09665"
                     /inference="COORDINATES: protein motif:HMM:NF017426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF779 domain-containing protein"
                     /protein_id="HNX71553.1"
                     /translation="MVERVVATPVATELIERLLVQHGPLLFHQSGGCCDGSAPMCYPQ
                     GEFHLGHQDVLLGEIVGCPFYMSAFQFDYWRNTHLTIDVVEGRGSSFSVEAPEGVRFL
                     TRSRLFTDDELAALEAAGEPPRADELTG"
     gene            509..937
                     /gene="pedF"
                     /locus_tag="PKN55_09670"
     CDS             509..937
                     /gene="pedF"
                     /locus_tag="PKN55_09670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008952536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-550 PedF"
                     /protein_id="HNX71554.1"
                     /translation="MKHPLRLLSLALAAAGALVVMGAASAHGDVVPQPVDTGNLKKLG
                     ATWLDSNPYRGNADAIHIGKAAFAQNCARCHGIDAVSGGIAPDLRKLPLQDEGDPIFQ
                     ERIRHGSVRNGVTYMPSFEGVLSQEAAWAIRSWIETVHMD"
     gene            1127..1951
                     /locus_tag="PKN55_09675"
     CDS             1127..1951
                     /locus_tag="PKN55_09675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014088309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HNX71555.1"
                     /translation="MNRRHFLLAALACACAAPVWAAPNLDDLKAAGGLRVALYRDFPP
                     YSWQEAGEMRGIDADLARAIGRELGLKVTFMPLTPADDVDGDLRNAIWKGHYLGGGTA
                     ELMLRVPFDPEFAQRNHNAVLFGAYASEQLAWAGQRPTAMTLIDRASRIGVENDSLAD
                     LYLSSLQGGRLRDSIKHYPNSPAAARALLAGDVDAVFAPQGELDWALAGTGQRIEPLA
                     APGLIKSQWEIGMATKESLRDLAWAVTDVIEALRQSGELERIYQRHQVRFLPPAAH"
     gene            2034..2798
                     /locus_tag="PKN55_09680"
     CDS             2034..2798
                     /locus_tag="PKN55_09680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014088310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein dehydrogenase-associated SoxYZ-like
                     carrier"
                     /protein_id="HNX71556.1"
                     /translation="MQWLRLICLAALCSALPTWAAPAGDPFNSPLWPNMHKLMLGGGK
                     VVFDPKVKVTIPERVEDGHFVPVTVDARELGEVRELVLFADYNPIPRALRYQPLAAQP
                     RLSVAMRVNQATPIRAAALDGKGVWHVSGQWLDAPGGGCALPSQTRSTTDWNSLLGRV
                     SGRAWREDGALRVRLGVMHPMDTGLVSNVARFNIEQLDIRDADNRLLARLETDASLAE
                     NPLFTLHLRPTPTPLLRASSQDNDGNHYTATLPLAP"
     gene            2921..3853
                     /locus_tag="PKN55_09685"
     CDS             2921..3853
                     /locus_tag="PKN55_09685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008952533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein relay system zinc metallohydrolase
                     1"
                     /protein_id="HNX71557.1"
                     /translation="MPTLRLCLAWCALLLAPLAAALELRPQAIAPDTWMLEADTGYFT
                     PQNGGFLVNTAFIATDEGVVVIGSGPSRQFGERYRKLIEDTTGKRVLEVIITHKHPDH
                     FLGNQAFRDVPIRADAKTTAMIKAEGEGLAENLYRLVGDGMRGTEALAPTAPLNVGVR
                     TIGGHRLEFIAVPGHTQSDIAVFDHTTGVLFAIDQVFNQRTPTLPNADLAQWRQSLAQ
                     LAAVPFKLLVPGHGPVSRDAAPIAATRDYLDWLDATLRDAAERGLAPTEVMLLPIPER
                     FAHWGEARAEWRRSIGFMYGRYERAALPWLSPAR"
     gene            complement(3927..5381)
                     /locus_tag="PKN55_09690"
     CDS             complement(3927..5381)
                     /locus_tag="PKN55_09690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HNX71558.1"
                     /translation="MAGEWVAELLVGRIQRTEPASLSRQLYTLLRAAMLDGRLPADTR
                     LPSSRWLAKELGLARNTVLEVYEQLLAEGYLRARRGAGSFVEAGLLRAASAAPRRAPA
                     LSQRGQTLIDCGPTPLALDGAFAPGVPALSAFPRQRWQRALNRHLRHAPAHWLGYQAQ
                     GGLPALREALAAYLGQSRGVRCQPEQLLITGGAQQALDWLARLLADVGDVAWMEDPGY
                     LGARSALTAAGLQLHPAPVDASGLDVAYAQAQLPAPRLIYVTPSHQYPGGAVMPLARR
                     QALLACAEAHGAWIIEDDYDSEFRYASQPVAALQGLADSARVIYLGTLSKVMCPALRL
                     AYLVVPESLIDAFRRASWHLAREGHYPTQAALADFISSGELARHIRRMRELYQSRQQA
                     LRQAVTQQLGAALPLSGGEAGMHVLAHLPAEIDDVALARRGWLEQQLVLRPLSSHSLR
                     TPAPRGWLLGYAGVEESAMAPAVARMARLLEGVW"
     gene            5524..>6109
                     /locus_tag="PKN55_09695"
     CDS             5524..>6109
                     /locus_tag="PKN55_09695"
                     /inference="COORDINATES: protein motif:HMM:NF024304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HNX71559.1"
                     /translation="MSQLTVTDLTRVRRHPERAHYDRDTLHALLDAALICHIAFVLDG
                     QVQQIPTACWREGEHLYIHGSNGSRMIRALRAGASVCVAVSLFDGLVLAKSSFSTSVN
                     YRSALIYGQFAALEGEDAKRASLKAFFEHYLPGRWDEVREPDASELAATTVLALPLEQ
                     AVCKVRSGPPQDKAEDEAAPVWAGVLPALSGWGAP"
BASE COUNT         1015 a         2064 c         1994 g         1036 t
ORIGIN      
        1 cccctgtccg cagggttgcg cggacaccgt gccgccggta tggccggcaa gaggagcatc
       61 gtcatggttg aacgcgtggt tgccacccct gtggccacag aattgatcga acgtttgctt
      121 gttcagcatg gcccgctgct gtttcaccag tctggcggat gctgtgatgg cagcgcgccg
      181 atgtgttacc cgcaaggcga gtttcacctg ggacatcagg atgtcttgct gggcgaaatt
      241 gtgggctgtc cgttttatat gagcgccttt cagtttgact actggcgcaa tacccacctc
      301 accatcgacg tggtcgaagg ccgaggttca agtttctctg tggaagcgcc cgagggcgtg
      361 cgctttctca cccgctcgcg gctgttcacc gatgatgaac tggccgccct ggaagccgcc
      421 ggcgagccgc cgcgcgctga cgaattgacc ggctgacgcc acatcagccg acttgcatcc
      481 ccccgatgac atgcgaagga tcactaccat gaaacaccct ctgcgtttgc tctccctggc
      541 gctggccgcc gctggcgccc tggtggtcat gggcgcggcg agcgcccacg gcgacgtcgt
      601 gccgcaaccg gtggacaccg gcaacctgaa aaaactgggc gcgacctggc tggacagcaa
      661 cccctaccgt ggcaacgccg atgccatcca cattggcaag gccgcgtttg cacaaaactg
      721 cgcgcgttgc cacggcattg acgccgtttc cggcggcatc gccccggacc tgcgcaagct
      781 gccgctgcag gacgaaggcg atccgatctt ccaggaacgc atccgccacg gctcggtgcg
      841 caatggcgtg acctatatgc cgtcgtttga aggcgtgctg tcgcaagaag ccgcctgggc
      901 gattcgcagc tggattgaaa ctgtccacat ggactaagca tcgcgcacaa aacccgtctg
      961 ctgttgttgt ctgaccttgc cgtgatctgc ggcaggcgcg cagcgccggg tttggtgagc
     1021 gacaagcggg ctggcgcttg cgccagccag cgccaatgca ttgccgccaa gaaagccggg
     1081 cccatgacgt ccggcgctgg cgtcggcgcg cacccgagga agacacatga accgccgcca
     1141 ttttctgttg gctgcgctgg cctgcgcgtg cgcggcgcca gtgtgggccg cccccaatct
     1201 ggacgacctg aaagccgccg gtggtctgcg cgtggctttg tatcgggatt ttcccccgta
     1261 ttcctggcag gaagccggcg agatgcgcgg catcgacgcc gatctggcgc gcgccattgg
     1321 ccgtgagctg gggctgaaag tcaccttcat gccgctgacg ccagcggatg atgtagacgg
     1381 tgatttgcgc aacgccatct ggaaaggcca ctacctgggc ggcggcactg ccgagctgat
     1441 gttgcgcgtg ccgttcgatc ccgaatttgc ccagcgcaac cacaatgccg tgctgtttgg
     1501 cgcttacgcc agcgaacaac tggcctgggc cggccagcgc cccaccgcga tgaccctgat
     1561 cgaccgcgcc agccgcatcg gcgtggaaaa cgacagcctg gcggatttgt atttgtcttc
     1621 cttgcaaggc gggcggctgc gcgacagcat caagcattat cccaacagcc ccgccgccgc
     1681 ccgcgccttg ctggccggtg atgtagacgc cgtgtttgcc ccacagggtg agctggattg
     1741 ggccttggct ggcactggtc agcgcatcga gccgctggcg gcgccgggcc tgatcaagtc
     1801 acagtgggaa atcggcatgg ccaccaaaga aagcctgcgc gacctggcct gggcggtgac
     1861 tgatgtgatc gaagccttgc gccaatctgg cgagctggag cggatttacc agcggcatca
     1921 ggtgcgcttt ttgccgccgg ctgcccattg agcgccgcta agcgtcgctc acaaaactcc
     1981 ctggcggcag tgcgctatgc cttcctcatc catgcgcttg caggagaacc accatgcaat
     2041 ggctgcgttt gatctgtctg gctgcgctat gcagcgcctt gcccacctgg gcggcgccgg
     2101 ctggcgaccc gtttaattcg cccttgtggc cgaatatgca caagctgatg ctgggcggcg
     2161 gcaaggtggt gttcgacccc aaagtcaaag tcaccatccc cgagcgggtg gaagacggcc
     2221 attttgtgcc ggtgacggtg gacgcccgcg agttgggcga ggtgcgcgag ctggtgctgt
     2281 ttgccgatta caacccgatt ccgcgcgcgc tgcgctacca gccgctggcg gcacagccgc
     2341 gcctgtcggt ggccatgcgc gtcaaccagg ccacgccgat tcgcgccgcc gcgctggacg
     2401 gcaagggcgt gtggcacgtc agcggccaat ggctggatgc gccgggcggc ggctgtgcgc
     2461 tgcccagcca gacccgctcc accacggact ggaacagcct gctgggccgg gtcagcggcc
     2521 gcgcctggcg cgaagacggc gcgctgcgcg tgcgcctggg ggtgatgcac ccgatggaca
     2581 ccggcctggt cagcaatgtc gcccgcttta atatcgaaca gctggacatc cgcgacgccg
     2641 acaaccgcct gctggcccgg ctggaaaccg acgcctcgct ggcggaaaac ccgctgttca
     2701 ccctgcatct gcgcccgacc cccacgccgc tgctgcgcgc cagcagccag gacaacgacg
     2761 gcaaccacta caccgccacc ctgccgctgg cgccataagc gtcgcttaca aaaccccgcg
     2821 aagcggccag acagtgcaag cagtacggca aggtcacacc acaacgccaa ggggagtgtc
     2881 agtggcgcta agccagctgc ttcttgcaag gagaacaact atgcccaccc tgcgcctgtg
     2941 cctggcctgg tgtgccctgc tgctggctcc gctggcggca gcgctggaat tgcgccccca
     3001 ggctatcgcc cccgacacct ggatgctgga agccgacacc ggttacttca ccccgcaaaa
     3061 cggcggcttt ttggtgaata cggcattcat cgccaccgat gaaggcgtgg tggtgattgg
     3121 cagcggcccc tcgcgccagt ttggcgagcg ctaccgcaag ctgattgaag acaccaccgg
     3181 caagcgcgtg ctggaagtca tcatcaccca caagcacccg gatcactttt tgggcaatca
     3241 ggcgtttcgt gacgtgccga ttcgcgccga cgccaaaacc acggccatga tcaaagccga
     3301 gggcgagggc ctggccgaga atctgtaccg gctggtgggc gacggcatgc gcggcaccga
     3361 ggcgctggcc cccaccgcgc cgttgaatgt gggcgtgcgc accattggcg gccaccggct
     3421 ggaattcatc gccgtgcccg gccacaccca atccgatatc gccgtgtttg atcacaccac
     3481 tggcgtgctg ttcgccattg atcaggtgtt caaccagcgc acccccaccc tgcccaatgc
     3541 cgatctggcg caatggcgac aatcgctggc gcaactggcc gccgtgccgt tcaagctcct
     3601 ggtgcctggc catggcccgg tcagccgcga cgccgcgccg attgccgcca cccgcgatta
     3661 tctggactgg ctggacgcca ccctgcgcga cgccgccgag cgcggcctgg ccccgaccga
     3721 agtcatgctg ctgcccatcc ccgagcgctt tgcccactgg ggcgaggcgc gcgccgaatg
     3781 gcggcgcagc attggtttta tgtatggtcg ctatgagcgc gcagccttgc cctggttgag
     3841 cccggcacgt taagagtcgc tcaccaaatc ctccaggcgt tattgtggtc tggccaggcg
     3901 cacggcgctg agtgtggtga gccggctcac cacaccccct ccagcagccg cgccatgcgc
     3961 gccaccgccg gcgccatggc gctttcttcc accccggcgt agcccaataa ccagccgcgc
     4021 ggcgctggcg tgcgcaggct gtggctggat aagggccgca gcaccagttg ctgctccagc
     4081 cagccgcgcc gcgccagcgc cacatcgtcg atctccgccg gcaagtgcgc cagcacatgc
     4141 atgccggcct ccccgcccga cagcggcagc gccgcgccca gctgttgcgt caccgcctgg
     4201 cgcagcgcct gctgacggct ttggtacagc tcgcgcatgc ggcggatatg ccgcgccagc
     4261 tcgccgctgc tgatgaaatc cgccagcgcc gcctgcgtcg ggtaatgccc ctcgcgcgcc
     4321 agatgccagc tggcgcggcg aaaagcgtcg atcaggcttt ctggcacgac caaatacgcc
     4381 aggcgcaagg ccgggcacat caccttggat aaggtcccca gataaatcac ccgcgcgctg
     4441 tcggccagcc cctgcaaggc ggccaccggc tggctggcgt agcggaactc gctgtcgtaa
     4501 tcgtcttcga taatccacgc gccatgcgct tcggcgcagg ccagcaaggc ttgccggcgc
     4561 gccagcggca tcaccgcgcc gccggggtat tggtgcgagg gggtgacgta aatcagccgg
     4621 ggcgcgggca gctgcgcttg cgcataagcc acgtccagcc cgctggcgtc cactggcgcc
     4681 gggtgcagct gcaaaccggc ggcggtcagc gcgctgcgcg cgcccagata accggggtct
     4741 tccatccacg ccacatcgcc gacatcggcc agtagccgcg ccagccaatc cagcgcctgc
     4801 tgcgcgccgc cagtgatcag cagctgttcg ggctggcagc gcaccccgcg cgactggccc
     4861 agatacgccg ccagcgcctc gcgcagcgcc ggcaggccgc cttgcgcctg gtagcccagc
     4921 cagtgcgctg gcgcgtggcg caaatggcga ttgagcgcgc gctgccagcg ctggcgcgga
     4981 aacgccgaca aggccggcac gcccggcgca aacgcgccat ccagcgccag cggcgtcggc
     5041 ccgcagtcga tcagcgtctg cccgcgctgc gacagcgccg gggcgcgcct gggcgcggcg
     5101 ctggcggcgc gcagcaggcc ggcttcgaca aaactccccg ccccacgccg cgcgcgcaga
     5161 taaccctcgg ccagcagttg ctcgtacacc tccagcacgg tgttgcgcgc caggccaagt
     5221 tctttggcca gccagcgcga cgagggcagg cgcgtgtcgg ccggcaaacg gccatcgagc
     5281 atggcggcgc gcagcagggt gtaaagctgg cggctgaggc tggcgggctc ggtgcgctga
     5341 atgcggccga ctagcaattc ggccacccat tctccggcca tgattggctc cctgattgag
     5401 tcaaaattgg ctcttattat gcagccaaag gtaggcgaag atggggcgtg atctggggcg
     5461 agctggcagg cggtgtatgc caatcccccg gtgttatctt gctttgaccc aaggagcgtt
     5521 gccatgagcc aactgaccgt gactgatctg acccgagtgc gccgtcaccc ggagcgcgcg
     5581 cattatgacc gcgacaccct gcatgcgctg ctggacgcgg cgctgatttg ccacattgcc
     5641 tttgtgctgg atggccaggt gcagcagatt cccaccgcct gctggcggga aggtgagcat
     5701 ttgtatattc acggctcgaa tggcagccgg atgatccgcg ccttgcgcgc cggggcaagt
     5761 gtgtgcgtgg cggtgagttt gtttgatggg ctggtgctgg ccaagtcgtc gttttcgact
     5821 tcggtgaatt accgctcggc gctgatttac ggccagtttg ccgcgctgga aggggaagac
     5881 gccaagcgcg ccagtctcaa ggcgtttttt gagcattatc tgcccggccg ctgggatgag
     5941 gtgcgcgagc cggacgccag cgagctggcg gccaccacgg tgctggcctt gccgctggag
     6001 caggcggtgt gcaaggtgcg cagcggcccg ccgcaggaca aggcggaaga cgaagccgcg
     6061 ccggtgtggg ccggggtgtt gccggccttg agcggctggg gcgcgccgc
//
LOCUS       DAOPYX010000183         6100 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_15996_length_6100_cov_8.654591, whole genome shotgun sequence.
ACCESSION   DAOPYX010000183 DAOPYX010000000
VERSION     DAOPYX010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 6100)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6100)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6100
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15996_length_6100_cov_8.654591"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            416..1672
                     /locus_tag="PKN55_09700"
     CDS             416..1672
                     /locus_tag="PKN55_09700"
                     /inference="COORDINATES: protein motif:HMM:NF024700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNX71560.1"
                     /translation="MRIDRIDIDNFCGFVHGEFRFSPGFNLLVGVNGSGKSSLLQALA
                     VGLQPLLRHVAAPAACQPQARVALLESGGRVRHERCYPVRLSLAAELAGSACAWVLEM
                     SGPGCAWQDSGEAPDLASWVREARTLPLIACYPAERHWRLPQVSADSAMRQQESRLHG
                     YQDWRDAAADTRGLESWVIGKTLERLEAVAMGEATAAADELERVNQVIRLAMPGARGL
                     RFDVKYRQLMLDWTQAACAPFDTLSDGQRGMVALLADIARRMCLLNPQLGDAVLSDTP
                     GVVLIDELDLHLHPAWQRRLPGVLKRAFPRVQFIAASHSPQMIGELPFDEIWLMRDNQ
                     ALGHPEHALGLSSNQVLEQLMDSAPRNPRIDEALREIDQLIDADHIGDAQTRLDTLAD
                     EVGQIPEVVGAQSAIDTLRWLEDDPA"
     gene            1669..2388
                     /locus_tag="PKN55_09705"
     CDS             1669..2388
                     /locus_tag="PKN55_09705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX71561.1"
                     /translation="MRSIDKGPEPAELSAWKARNAATPRHLRYDGIGFPREAVRRALL
                     LEQRHLCAYTMQALLTAKQCERRGWHTGFACHIEHFLPQARQIAAESIDYRNMLACHP
                     PSMPGVYCGYGAQHKAAYDPAGQAFVSPLSADAASHFAFHGDGRVLGLSEAGRNSVRV
                     LNLNHPALVHARQAAISHALRPRANVSLSPQAARKLAAAVLRAEGRERLPAYCVAIAQ
                     AALKYAEREERRAARRAAAPR"
     assembly_gap    2474..2573
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2630..3886)
                     /locus_tag="PKN55_09710"
     CDS             complement(2630..3886)
                     /locus_tag="PKN55_09710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012698260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HNX71562.1"
                     /translation="MNLRTRFTLLCTALALAACATRAPAPTAPARPPVSSKPTPLPSA
                     SRPAGPVVKAPVPASDADAKYIPVTWAALPGWREANLAEGFSGLLESCKALGKRAGWR
                     PSCEAAAQIDPNNGEAVRAFYETRFTPYRLSDGDKMLGLVTGYYEPLLRGDTQRSERG
                     RYPVYAPPPDLLTIDMASMYPELKNLRLRGRVQGNKVVPYWTRTDIESGRGVERAEVL
                     AWVEDPIELFFLQVQGSGRIELPSGELLRVGYADQNGYPYKSIGKWLIDKGELTAGQA
                     TMQGIQAWAQANPARLTELLNSNPSYVFFRRLNNNQGGPLGALGVPLTDGYSIAVDAR
                     YTPLGAPVWLSTSFPLGNQPLQRLMHAQDTGGAIRGAVRADFFWGFGREAGEVAGRMK
                     QAGEMWILLPKGMSPSQALAGGKASG"
     assembly_gap    3998..4097
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4098..>6100)
                     /locus_tag="PKN55_09715"
     CDS             complement(<4098..>6100)
                     /locus_tag="PKN55_09715"
                     /inference="COORDINATES: protein motif:HMM:NF028510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNX71563.1"
                     /translation="TMKIGVKLALAQSLLLLALGLAFAMAVSMQLGERLRARSDAVQD
                     RVVEDALGMLDAYNQALKSSVDQYARMFESELIGQFSLDTQRTMPLGQAQVGILRLDN
                     TVLNQRVDEVDRFTARTGAVATVFARAGEQLLRVTTSLRQQDGQRALGTALDPDSPAY
                     KNLMAGLPYIGKVRLFGRDYMARYTALENSQGQVIGATFVGVDITQDMVNLRARLAKV
                     KVGDSGHVHVLALAGPERGQWLLHPEHSGRPAAAQRDAHDQPAYAALLAGAAGAQAVS
                     LADGEHLLRYAPYPDWQWLLVSDELADEANAENRHILLWLLGGVGVLLVCLVAAMALT
                     THRLVSRPLGEAAGALRRISQARDLTARLPTRGRDEISALAQAFNGLQDALQHALCAT
                     HDGAQQVDAAARGVSDSARQVALDSHRQSQAAQSMAGEIDQLTQAIADIAHDTRQAHA
                     LSAQAAQRASHSQQAVASAIEQVGEVASTLSGAASTLGAARQQAEQISAIVNVIDDIA
                     EQTNLLALNAAIEAARAGEQGRGFAVVADEVRKLAERTQGATHEISDMISGMQQSSRH
                     AASAMQAAVSRVDQGVAATRQVGEVIGELQHGAEHAARLMSAVSARLDAESDAASGIG
                     QHTHQVVDMADANQAAAQHAATAASQMTQMAALLRQEVERFRV"
BASE COUNT          897 a         1918 c         2013 g         1072 t
ORIGIN      
        1 tgctcccggg ctggctgcga tgctcggcgc gctttacggg gaggtaaagc ggctaagcgc
       61 gacgggtcag gcattgaaaa attgtgtgtt tttgtggcgt ttggcctggt tttgcctaaa
      121 aaaagaattt atccttgaag ctcatgagct tgtggcatag atcctctacc ttgccaccgc
      181 ccgtccctct ccccccgcag taccccaccg agtcattccc gcgcaggcgg gaatccagac
      241 gcatccatag cgtaccgcag gccagtcggt tcatttcatg gtgactgact actagacgcc
      301 gccggtgccc cctcccgctc cgcagatttg ctatgctgca ttcgcccgcc atgctggcgc
      361 gcggttttcc atttccctgt ttgctgactg actgcattgg ccggagtttg ccttcatgcg
      421 cattgaccgc atcgacatcg ataatttttg cgggtttgtt cacggcgagt ttcgtttttc
      481 gccggggttt aacctgttgg tcggggtgaa tggcagcggc aagagttctt tgctgcaagc
      541 cttggcggtg ggtttgcagc ctttgttgcg ccatgtggcg gcgccggcgg cgtgtcagcc
      601 gcaggcgcgg gtggcgttgt tggagagcgg cgggcgggtg cggcatgagc ggtgttatcc
      661 ggtgcgcttg agcctggcgg ctgagctggc cgggtcggcg tgcgcctggg tgctggagat
      721 gagcgggccg ggctgtgcct ggcaggacag cggcgaggcg ccggatttgg ccagttgggt
      781 gcgcgaggcg cgcactttgc cgctgattgc ctgttatccc gccgagcggc attggcgttt
      841 gccgcaggtg tcggcggaca gcgccatgcg ccagcaggag tcgcgcttgc atggctatca
      901 ggattggcgc gacgccgccg ccgacacgcg cgggctggaa agctgggtga ttggcaaaac
      961 gctggagcgg ctggaggcgg tggccatggg cgaggcgacg gcggcggcgg atgagctgga
     1021 gcgggtgaac caggtgattc ggctggcgat gccgggtgcg cgcggcttgc gctttgatgt
     1081 gaaataccgg cagttgatgc tggattggac gcaggcggcg tgcgcgccgt ttgacacgct
     1141 cagcgatggc cagcgcggca tggtggcctt gctggccgat attgcccggc gcatgtgcct
     1201 gcttaacccg cagctgggcg acgcggtgct gagcgacacg cccggtgtgg tgttgatcga
     1261 cgagctggat ttgcacctgc acccggcctg gcagcgtcgc ttgccggggg tgttgaagcg
     1321 ggcgtttccg cgcgtgcagt ttattgccgc ctcgcattcg ccgcagatga ttggcgagct
     1381 gccgtttgat gaaatctggc tgatgcgcga caaccaggcg ctgggccatc ccgagcatgc
     1441 gctggggctg agcagcaatc aggtgctgga acaattgatg gacagcgcgc cgcgcaatcc
     1501 gcgcatcgac gaggcgctgc gcgaaattga ccagctgatc gacgccgacc atatcggcga
     1561 cgcgcaaacc cggctggaca ccctggccga tgaggtgggg cagattcccg aggtggtcgg
     1621 cgcgcaaagc gccatcgaca ccttgcgctg gctggaggac gacccggcgt gagaagcatc
     1681 gacaaggggc cagaaccggc cgagctgagc gcctggaagg cgcgcaacgc cgccaccccg
     1741 cgccatctgc gctacgacgg catcgggttt ccgcgcgagg cggtgcgccg cgccttgctg
     1801 ctggagcagc gccatttatg cgcctacacc atgcaagcct tgctcaccgc caagcagtgc
     1861 gagcggcgcg gttggcacac tggctttgcc tgccatatcg agcacttttt gccgcaggcg
     1921 cgccagattg ccgccgaaag cattgattac cgcaatatgc tggcctgcca tccgcccagc
     1981 atgccgggtg tgtattgcgg ctacggcgca cagcataaag ccgcctacga cccggctggc
     2041 caagcctttg tctcgccctt gtcagccgac gccgccagcc attttgcctt tcatggcgat
     2101 ggccgcgtgc ttgggctgag cgaggccggg cgcaacagcg tgcgcgtgct caacctcaat
     2161 cacccggcgc tggtgcatgc ccgccaggcg gccatcagcc acgccttgcg cccgcgcgcc
     2221 aatgtcagcc tgtcgccgca ggcggcgcgc aagctggcgg cggcggtgct gcgcgccgaa
     2281 ggccgcgagc ggctgccggc gtattgcgtg gcgattgctc aggccgcgct gaaatacgcc
     2341 gagcgcgaag aacgccgtgc ggcgcggcgg gcggcagcgc cgcgctgaac cacaggccac
     2401 aggcgctacc cgctcaatcc ggcccgtggc gctcagccct gctctcaggc cggttaagag
     2461 ccgctcacaa aacnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnngctaagc
     2581 cgccctggca agcgtaccgc cgcaaaaggg gtgggtcagg caggttttgc taaccgctcg
     2641 ccttgcctcc agccagcgcc tggctggggc tcataccctt gggcagcaga atccacattt
     2701 ccccggcctg cttcatccgc ccggccacct caccggcttc acggccaaag ccccagaaaa
     2761 agtctgcccg caccgcacca cgaatggcgc cgcccgtgtc ctgcgcgtgc atcagccgtt
     2821 gcagtggctg attgcccagc ggaaaactgg tagacagcca caccggggcg cccagcgggg
     2881 tatagcgggc atccacagca atgctgtagc catcggtcag cggtacgccc agtgcgccca
     2941 gcgggccgcc ctggttgttg ttgagccgac ggaagaacac atagctgggg ttgctgttca
     3001 atagctcggt cagccgcgcc gggttggcct gcgcccaggc ttgaatgccc tgcatggtgg
     3061 cctggccggc ggtcagctcg cctttgtcga tcagccattt gccaatggat ttgtacggat
     3121 agccgttctg gtcggcgtag cccacccgca gcagctcgcc actgggcagc tcaatgcggc
     3181 cggaaccctg cacttgcagg aaaaacagct caatcggatc ttccacccag gccagcacct
     3241 cggcgcgctc gacgccacgg ccgctttcga tgtcggtgcg tgtccagtat ggcaccacct
     3301 tattgccctg cacccggccg cgcaggcgca gatttttcag ctcgggatac atactggcca
     3361 tgtcgatggt cagcaaatcc ggcggcggcg catacaccgg gtaacggccg cgctcgctgc
     3421 gctgggtgtc gccgcgcaac agtggctcgt aatagccggt gaccaggccc agcattttgt
     3481 cgccgtcgct gaggcggtag ggggtaaagc gggtttcgta aaaggcgcgc accgcttcgc
     3541 cgttattcgg gtcgatctgg gccgccgcct cgcagctggg ccgccagccg gcgcgcttgc
     3601 ccagcgcctt gcagctttcc aacagtccgc tgaaaccttc agccagattg gcctcgcgcc
     3661 agcccggcaa ggccgcccag gtgacgggga tatatttggc gtcggcatcg ctggccggaa
     3721 ccggcgcctt caccaccggg ccggccggac ggctggcgct gggcaagggc gtgggtttgc
     3781 tgctgaccgg gggacgtgca ggcgcagtgg gggctggcgc gcgcgtggca caggcggcca
     3841 aggccagcgc agtgcacaac agggtaaaac gggtgcgaag attcatgacg gcagcggggg
     3901 ctctggttca aacaggagtt caagattgta accgagccac gacaggacgg cagcgcggct
     3961 gtcggttttt gccacgctgg cgtcgcttat cgggtgcnnn nnnnnnnnnn nnnnnnnnnn
     4021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4081 nnnnnnnnnn nnnnnnncac ccgaaaacgc tccacttcct gccgcagcag agcggccatt
     4141 tgcgtcatct ggctggcggc ggtggcggcg tgctgggcgg cggcctggtt ggcgtcggcc
     4201 atgtccacca cttggtgggt gtgctggcca atgccgctgg cagcgtcgct ttcggcatcc
     4261 agccgggcgc tgaccgcgct catcagccgg gcggcgtgct cggcgccgtg ttgcagctcg
     4321 ccaatcactt cgcccacctg gcgggtggcg gccacgccct ggtccacccg gctgaccgcc
     4381 gcttgcatgg cgctggcggc gtggcggctg ctttgctgca tgccggagat catgtcgctg
     4441 atttcatggg tggcgccttg ggtgcgctcg gccagcttgc gcacttcgtc ggccaccacg
     4501 gcaaaacctc ggccctgctc gccggcgcgg gcggcttcga tggcggcgtt caaggccaag
     4561 agattggttt gttcggcgat gtcgtcaatc acattgacaa tggcggaaat ctgctcggcc
     4621 tgctggcggg cggcgcccag ggtgctggcg gcgccggaca gcgtgctggc cacttcgccc
     4681 acttgctcaa tggcgctggc caccgcctgc tggctgtggc tggcgcgctg ggcggcttgc
     4741 gcgctcaagg cgtgcgcttg ccgggtgtcg tgggcgatgt cggcaatcgc ctgggtgagc
     4801 tggtcgattt cgccggccat gctttgcgcc gcctggcttt gccggtgcga atccagcgcc
     4861 acctggcggg cgctgtcgct gacgccgcgc gcggcggcgt ccacttgctg ggcgccgtca
     4921 tgggtggcgc acagcgcgtg ttgcagcgcg tcttgcaggc cattaaacgc ctgcgccagc
     4981 gcgctgattt catcgcggcc gcgcgtgggc agccgggcgg tgaggtcgcg cgcctggctg
     5041 atgcggcgca gcgcgccagc ggcttcgccc aagggtcggc tgaccaggcg atgggtggtc
     5101 agcgccatcg ccgccaccag gcacaccaga agcacgccca ccccgcccag cagccacagc
     5161 agaatatggc gattctcggc attggcttcg tcggccaatt cgtcgctgac cagcagccat
     5221 tgccagtccg ggtaaggcgc atagcgcagc agatgctcgc cgtcggccaa tgacacggcc
     5281 tgggcgccgg ccgcgccggc cagcagcgcc gcgtaggcgg gttgatcatg cgcatcgcgc
     5341 tgggcggcgg ccgggcggcc gctgtgctcg gggtgcagca gccactggcc gcgctcgggg
     5401 ccggccagcg ccagcacatg cacatggccg ctgtcgccca ccttgacctt ggccagccgc
     5461 gcgcgcaggt tgaccatgtc ctgggtgatg tccacgccca caaacgtggc gccaatcacc
     5521 tggccctggc tgttttccag cgcggtgtag cgcgccatgt aatcgcggcc aaacaagcgc
     5581 accttgccga tatacggcaa gccggccatc aggtttttat aggccgggct gtccgggtcc
     5641 agcgccgtgc ccagcgcgcg ctggccatcc tgctggcgca gcgaggtggt cacccgcagc
     5701 aattgctcgc cggcgcgggc aaacaccgtg gccactgcgc cggtgcgggc ggtaaagcgg
     5761 tccacctcgt ccacccgctg gttcagcacg gtgttgtcca gccgcaagat gcccacttgc
     5821 gcctgaccca gcggcatggt gcgctgggta tccaggctga actggccaat cagctcggat
     5881 tcaaacatgc gcgcgtattg gtctacgctg cttttcagcg cctggttata ggcgtccagc
     5941 atgcccagcg cgtcttccac caccctatcc tgcaccgcgt cactgcgcgc gcgcaggcgc
     6001 tcgcccaact gcatcgacac cgccatggca aaggccaggc ccagcgccag caatagcagg
     6061 ctctgagcca gcgccagctt gactccgatt ttcatggtgt
//
LOCUS       DAOPYX010000184         6087 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_16047_length_6087_cov_9.850298, whole genome shotgun sequence.
ACCESSION   DAOPYX010000184 DAOPYX010000000
VERSION     DAOPYX010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 6087)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6087)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6087
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16047_length_6087_cov_9.850298"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            135..320
                     /gene="nrdD"
                     /locus_tag="PKN55_09720"
     CDS             135..320
                     /gene="nrdD"
                     /locus_tag="PKN55_09720"
                     /EC_number="1.1.98.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008952378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HNX71564.1"
                     /translation="MPTPHTTPSNPITLTPHDPRRTRCEIWTRVMGYHRPVSSFNTGK
                     QGEFHERRFFREPQRHD"
     gene            280..984
                     /locus_tag="PKN55_09725"
     CDS             280..984
                     /locus_tag="PKN55_09725"
                     /inference="COORDINATES: protein motif:HMM:TIGR02495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0051353 - positive regulation of
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HNX71565.1"
                     /translation="MNGASSVNPSATTEPAIGGLTPFSSVDWPGQLAAVVFIAGCPWR
                     CGYCHNPQLQPRQRTLDWPAILHWLDSRRGLLDGVVFSGGEPLSEPALADMARAVQAR
                     GFAVGLHSAGIYPQRLAQLLPWLDWLALDIKTDQAGYDALTGRARSAWPVQVCLEMAL
                     EAGCALECRTTWSPQRLSEARLLALAQTLAARGVRHYAVQDERSAPGQPAQGTLSEAA
                     QAQLARCFASFSYRRS"
     gene            complement(1021..1866)
                     /gene="cobA"
                     /locus_tag="PKN55_09730"
     CDS             complement(1021..1866)
                     /gene="cobA"
                     /locus_tag="PKN55_09730"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008953031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HNX71566.1"
                     /translation="MNAPCPLPPLAGGALIDDEAWPTGVQVALVGAGPGDAGLLTLRA
                     RARLRAAEVVLYDHLINPAILALAAQARLCCVGKRAGAHSVGQRQIEQLLIDEARAGR
                     RVVRLKGGDPLLFGRGGEEMQALAAAGLRYEVTPGVSAALGAAAMAHLPLTHRDHAQG
                     CTLVTGHRRDDASELAWTAHTHPGATVVVYMGVRQAGAIAQALMQHGRAADTPAAVIE
                     AATTPRQRVIRTRLDQLGAAVAEHDAHTPALLVIGPVLAVHDELMAAVALSAADNTPR
                     SDQCG"
     gene            2018..2443
                     /locus_tag="PKN55_09735"
     CDS             2018..2443
                     /locus_tag="PKN55_09735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008953030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HNX71567.1"
                     /translation="MSASFTKSTARNIFYGGAVFFFLLFLALTVDTVQTLPKRDNRAN
                     LTEQVARGKKLWEVNNCIGCHTLLGEGAYFAPELGNVYQRRGPEFIKAWIKAQPTGAP
                     GRRQMPNFHLNDAELDDLVAFLKYASEIDTNQWPPNIEG"
     gene            2481..3920
                     /locus_tag="PKN55_09740"
     CDS             2481..3920
                     /locus_tag="PKN55_09740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017507114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HNX71568.1"
                     /translation="MSTSAKAAPRPAGGPTPANMHIRFASQTVAKPYFVAAIGLFVGQ
                     VIFGLIMGLQYVIGDFLFPEIPFNVARMVHTNLLIVWLLFGFMGAGYYLIPEESEREL
                     HSPKLAIITFWVFLVAGALTIVGYLAVPYATLAKITGNDLLPTMGREFLEQPTITKIG
                     IVLVALSFLFNLSMTVLKGRKTSISLVMMLGLWGLAIFFLFSFYNPHNLVLDKYFWWW
                     VVHLWVEGVWELIMGAMLAFVLVKVTGVDREIIEKWLYVIIAMTLITGIIGTGHHYFW
                     IGTPAYWQVWGSVFSALEPIPFFMMTLFAFNMVNKRRREHPNKAAVLWALGTGVMAFL
                     GAGVWGFLHTLAPVNYLTHGTQITASHGHMAFYGAYAMVVMTMISYTMPLMRGREANS
                     NGAQVVEMWAFWLMTIAMVFITLFLTGAGILQVWMQRMGEQPLGFMATQDKIAFFYWL
                     REAAGVVFFAGLLLYIVSFFVGKREVARG"
     gene            4072..4635
                     /locus_tag="PKN55_09745"
     CDS             4072..4635
                     /locus_tag="PKN55_09745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018151802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HNX71569.1"
                     /translation="MPRSRHVPGDLAMWLFILAELAVFALFFALYGVHRARQPALFAQ
                     GQASLRLGSALVNTLLLIAGSAAVAASVRAFAANQLARGARLLGWTWLSGAVFLLLKG
                     EEFAHLFAAGVSLSSDDFWMFYLSLTVFHFLHVVLGMVIIAAVWLRARRGAYGAADHV
                     GVETAAAYWHMVDLVWVVLFALVYVAR"
     gene            4825..5175
                     /gene="arsC"
                     /locus_tag="PKN55_09750"
     CDS             4825..5175
                     /gene="arsC"
                     /locus_tag="PKN55_09750"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018746396.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HNX71570.1"
                     /translation="MTVCIYHNPRCSKSRDTLALLHAQGLTPTVVAYLDTPPDAATLR
                     EVLAKLGLSDARALMRKGEAEYTDLHLADPSLSQEALIAAMVAHPRLIERPIVVNGNK
                     AAIGRPPEQVLAIL"
     gene            5239..>6087
                     /gene="argC"
                     /locus_tag="PKN55_09755"
     CDS             5239..>6087
                     /gene="argC"
                     /locus_tag="PKN55_09755"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: protein motif:HMM:TIGR01851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HNX71571.1"
                     /translation="MSYKVFVDGQEGTTGLQINEYLAKRDDITLLKIDSDKRKDLAER
                     QRLINESDVTFLCLPDAAAKESVSLVNNPNTCVIDASTAHRVNPDWVFGLPELAADQR
                     DKIRASKRIANPGCHASAFILALRPLVAAGLLPADSQIAANSITGYSGGGKSMIAEYE
                     SPERILAPRAYALGLAHKHLPEMQAYTGLSVAPIFQPIVGPFYKGLAVTCYLHPQQFS
                     RAATPQQVRELLADYYQGERFIRVLPFDLNATTDGGFYNVEANNGSNHVDIAVYGTEE
                     RMLLIAR"
BASE COUNT         1045 a         2001 c         1893 g         1148 t
ORIGIN      
        1 ggggtcgcct ttctttgcct actttctttg gcgaagcaaa gaaagtaggg cggcacgcgg
       61 gacgcactcc cgcccaaaat caaccccgcg cgacagcgcg gctcaactca catttttaag
      121 gaaagacaag caccatgccc accccacaca ccaccccaag caatcccatc accctcaccc
      181 cacacgaccc ccgccgcacc cgctgcgaaa tctggacccg cgtcatgggc taccaccgcc
      241 cggtcagctc attcaacacc ggcaaacaag gcgaattcca tgaacggcgc ttcttccgtg
      301 aaccccagcg ccacgactga acccgccatc ggcggcctga cgcccttctc cagcgtcgat
      361 tggcccggcc agcttgccgc cgtggtgttt atcgccggct gcccatggcg ctgcggctac
      421 tgccacaacc cccaactgca accccgccag cgcacgctgg actggccggc catcctgcac
      481 tggctggaca gccgacgcgg cctgttggac ggcgtggtgt tctccggcgg cgagccgctg
      541 agcgaacccg cgctggccga catggcccgc gccgtgcaag cgcgcggctt tgccgtcggc
      601 ctgcacagcg ccggcatcta cccgcagcgg ctggcgcaac tgctgccctg gctggactgg
      661 ctggcgctgg acatcaaaac cgaccaggcc ggctacgacg cgctgaccgg ccgcgcgcgc
      721 agcgcctggc cggtgcaagt gtgtctggaa atggcgctgg aagccggctg cgcgctggaa
      781 tgccgcacca cctggagccc acagcggctc agcgaagcgc ggctgctggc gctggcgcaa
      841 acgctggcgg cgcgcggcgt gcgccactac gccgtgcagg atgaacggtc agcgccgggc
      901 cagccggcgc aagggacgct cagcgaggcg gcgcaggcgc agctggcccg ttgctttgcc
      961 agcttcagtt accggcgcag ctaagcgcgg cgaacacaag ccggctggcc ctgtgccgca
     1021 ctacccgcac tgatcgctgc gcggggtgtt gtccgcggcg ctcagcgcaa cggcggccat
     1081 cagttcatcg tgcaccgcca gcaccgggcc aatcaccagc aaggccgggg tgtgcgcgtc
     1141 gtgctcggcc accgccgcgc ccagttggtc cagccgggtg cggatcaccc gctggcgcgg
     1201 cgtggtggcg gcttcgatca ccgccgccgg ggtgtccgcc gcgcggccgt gctgcatcaa
     1261 ggcctgggca atcgccccgg cctgacgcac gcccatatac accaccacgg tggcgccggg
     1321 gtgggtgtgc gcggtccagg ccagctcgct ggcgtcgtcg cggcgatggc cggtgaccaa
     1381 ggtgcagccc tgggcgtggt cgcggtgggt gagcggcagg tgcgccatcg ccgccgcgcc
     1441 cagcgccgcg ctgacgccgg gggtgacttc atagcgcagc ccggcggcgg ccagcgcctg
     1501 catttcttcg ccgccgcgcc caaacagcag cgggtcgccg cccttgagcc gcaccacgcg
     1561 ccggccggcg cgcgcctcgt cgatcaataa ctgttcaatc tgccgctggc ccacgctgtg
     1621 cgcgccagcg cgcttgccca cgcagcacag ccgcgcctgc gccgccaggg cgaggatggc
     1681 cgggttgatc aagtggtcgt acagcaccac ctcagccgcg cgcaggcggg cgcgggcgcg
     1741 cagggtgagc aagccggcgt cgcccggccc ggcccccacc agcgccacct gcacgcccgt
     1801 tggccaggct tcgtcgtcaa tcagggcgcc gccggccagc ggcggcaagg gacagggggc
     1861 attcatcgtt gtttctccac aacacgagca tgccgccagt gtaggaattt cccggtcggg
     1921 aaatcgttga ttcgaatcaa tgctatccag atggccaaat ccgtacagtc catgctgaaa
     1981 ccggcgccat gccggagcgg actataggag ggtcgtgatg agcgccagtt ttaccaaatc
     2041 caccgcgcgc aatatttttt atggcggcgc ggtctttttc tttttactgt ttttagcgct
     2101 gaccgtggac accgtgcaaa ccctgcccaa gcgcgataac cgcgccaatc tcaccgagca
     2161 ggtggcgcgc ggcaaaaagc tgtgggaagt gaataactgc atcggctgcc acaccttgct
     2221 gggcgagggc gcgtattttg cgccggagct gggcaatgtc tatcaacggc gcggcccgga
     2281 gttcatcaaa gcctggatca aggcccagcc taccggcgcg cccggccgcc ggcaaatgcc
     2341 caattttcac ttgaacgacg ccgagctgga cgacctggtg gccttcctga aatacgcctc
     2401 ggaaatcgac accaatcaat ggccgcccaa tatcgaaggc tgatttcgtt ctattgccat
     2461 tagcatccca ggagaattcg atgtccactt ccgccaaggc tgcgccacgc cccgctgggg
     2521 ggccaacgcc agccaatatg cacatccgtt ttgcttcaca aacggtggcc aagccgtatt
     2581 ttgtcgccgc catcggcctg tttgtcggtc aggtgatttt tggcctgatc atgggcctgc
     2641 aatatgtgat cggtgatttt ctgtttccgg aaatcccgtt caacgtcgcc cgcatggtgc
     2701 acaccaatct gctgattgtc tggctgctgt tcggctttat gggcgccggc tactatttga
     2761 ttcccgagga atccgagcgc gagctgcaca gccccaaact ggccatcatc accttctggg
     2821 tgtttttggt ggccggggcg ctgaccattg tcggttatct ggcggtgcct tacgccacgc
     2881 tggccaaaat caccggcaat gatttattac ccaccatggg ccgcgagttt ctcgaacagc
     2941 cgaccatcac caagattggc atcgtgctgg tggcgctgtc gttcttgttc aacctgagca
     3001 tgaccgtgct caagggccgc aagaccagca tcagcctggt gatgatgctg ggcctgtggg
     3061 ggctggcgat tttctttttg ttctcgttct ataacccgca taacctggtg ctggataaat
     3121 acttctggtg gtgggtggtt cacctgtggg tggaaggcgt gtgggaactg atcatgggcg
     3181 ccatgctggc cttcgtgctg gtgaaagtga ccggcgtgga ccgtgaaatc atcgaaaaat
     3241 ggctgtatgt gatcatcgcc atgacgctga tcaccggcat catcggcacc ggccaccatt
     3301 acttctggat tggcacgccg gcttactggc aggtgtgggg ctcggtgttc tccgcgctgg
     3361 agccgattcc gttcttcatg atgaccttgt tcgccttcaa tatggtcaac aagcgccgcc
     3421 gcgagcaccc gaacaaagcc gccgtgctgt gggcgctggg cactggcgtg atggccttcc
     3481 tgggcgcggg cgtgtggggc tttttgcaca ccctggcccc ggtgaactac ctgacccacg
     3541 gcacgcaaat caccgccagc cacggtcaca tggccttcta cggcgcttac gccatggtgg
     3601 tgatgaccat gatctcgtac accatgccgc tgatgcgcgg acgcgaagcc aatagcaatg
     3661 gcgcgcaagt ggtggaaatg tgggcgttct ggctgatgac catcgccatg gtgttcatca
     3721 ccctgttcct gaccggcgcc ggcatcctgc aagtgtggat gcagcgcatg ggcgagcaac
     3781 cgctgggctt tatggccacg caggacaaaa tcgccttctt ctactggctg cgcgaagccg
     3841 ccggggtggt gttctttgcc ggcctgctgc tgtacatcgt cagcttcttt gtcggcaagc
     3901 gggaggtggc gcgcgggtaa gccgggcggc ggggaggtta accccacggt tggccgctgt
     3961 tcccccagcg cccatccagg ccactcaccc ccgccaacac ccaagctccc ctcatcctca
     4021 gatccaaccc tttggcgccc taccccgggc gctttttttc tggaggcgcg catgccgcgt
     4081 tcacgtcatg tgccgggcga cttggccatg tggttgttca tcctggccga gctggcggtg
     4141 tttgccctgt tttttgccct gtatggcgtt caccgcgccc gccagccggc gctgttcgcc
     4201 cagggccagg ccagtttgcg gctgggcagc gccttggtga acaccctgct gctgattgcc
     4261 ggcagcgcgg cggtggcggc cagtgtgcgg gcgtttgccg ccaatcagct cgcccgtggc
     4321 gcgcgtctgc tgggctggac ctggctgagc ggcgcggtgt ttttgctgct gaagggagaa
     4381 gaattcgccc acttgtttgc cgctggggtg agtttatcca gcgatgattt ctggatgttt
     4441 tacctgtcgc tgacggtgtt tcactttttg catgtggtgc tgggcatggt gattattgcc
     4501 gccgtctggc tgcgcgcccg gcgcggcgcg tatggcgcgg cggatcacgt cggggtggaa
     4561 accgccgcgg cgtactggca tatggtggat ttggtgtggg tggtgttgtt tgcgctggta
     4621 tacgtagcgc gttaagagtc gctaacaaaa cccagcgaag cgctcctggc taggcgtgtg
     4681 accgcagaca gtacaaggag tacggcaagg tcgcgcaacg acgccaggag ggttttgtta
     4741 gcggctctaa gaaggattga caatagcgcg tcagccccaa tcaggcgaca atgcgcgcca
     4801 attcccccac cggattgaac agccatgacc gtctgcattt accacaaccc ccgttgttcc
     4861 aaatcccgcg atacgctggc gctgttgcac gcccaggggc tgacgcccac ggtggtcgcc
     4921 tatctggaca ccccgccgga cgccgccacc ttgcgcgagg tgctggccaa gctggggctg
     4981 agcgatgccc gcgcgctgat gcgcaagggc gaggcggaat acaccgattt gcatctggcc
     5041 gaccccagcc tgagtcagga ggcgctgatt gccgcgatgg tggcgcaccc gcgcttgatc
     5101 gagcggccga ttgtggtcaa cggcaacaag gcagcgattg gccgtccgcc tgagcaagtg
     5161 ttggcgattc tgtaaaacac cccaggccgg ttttctgcca gaattctgtt tttacccacc
     5221 ctgacacgcg gattcaccat gagctacaaa gtctttgtcg acggccagga aggcaccacc
     5281 gggctgcaaa tcaacgaata cctcgccaag cgcgacgaca tcaccctgct caagatcgac
     5341 tctgacaagc gcaaggacct ggccgagcgc cagcgcctga tcaatgaatc cgacgtcacc
     5401 tttttgtgcc tgccggatgc ggcggccaaa gagtcggtga gcctggtgaa caaccccaac
     5461 acctgcgtga tcgacgcctc caccgcccac cgggtgaacc cggactgggt gtttggcctg
     5521 ccggagctgg ccgccgacca gcgcgacaag attcgcgcca gcaagcgcat cgccaacccg
     5581 ggctgccacg ccagcgcctt tatcctggcg ctgcgcccgc tggtggccgc cggcctgttg
     5641 ccggccgaca gccagattgc cgccaactcc atcaccggct attccggcgg cggcaagtcg
     5701 atgattgccg aatacgaaag cccggagcgc attctggccc cgcgcgccta cgcgctgggc
     5761 ctggcgcaca agcatctgcc ggaaatgcag gcctacaccg ggctgagcgt ggcgccgatc
     5821 ttccagccga ttgtcggccc gttctacaaa ggcctggcgg tcacctgcta tctgcacccg
     5881 cagcaattca gccgcgccgc caccccgcaa caggtgcgcg agctgctggc cgattactat
     5941 cagggtgagc gctttatccg cgtgctgccg tttgacctga acgccaccac cgacggcggt
     6001 ttttacaatg tcgaggccaa taacggcagc aatcatgtgg acatcgccgt gtacggcacg
     6061 gaagagcgca tgctgctgat tgcccgc
//
LOCUS       DAOPYX010000185         6044 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_16205_length_6044_cov_7.281683, whole genome shotgun sequence.
ACCESSION   DAOPYX010000185 DAOPYX010000000
VERSION     DAOPYX010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 6044)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6044)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6044
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16205_length_6044_cov_7.281683"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..371)
                     /locus_tag="PKN55_09760"
     CDS             complement(<1..371)
                     /locus_tag="PKN55_09760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018150498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HNX71572.1"
                     /translation="MFTLYIGNKNYSSWSLRPWLVMRHFDIPFRESRVALYQADSASK
                     LRAVSPSGKVPVLRDGELVVWDSLAIGEYLAERYPELGLWPGNARQRALARAVSAEMH
                     SGFTALRSQMPMNLRASLPGCG"
     gene            complement(711..1886)
                     /locus_tag="PKN55_09765"
     CDS             complement(711..1886)
                     /locus_tag="PKN55_09765"
                     /EC_number="4.2.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012697580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA];
                     GO_process: GO:0071268 - homocysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-succinylhomoserine sulfhydrylase"
                     /protein_id="HNX71573.1"
                     /translation="MSTTPLHPETLAIRAGKETSDYQEHSQAMFLTSSFKSASAAEAA
                     KLFSGEIPGYTYSRFTNPTVSAFQARLAALEGGERAVATSSGMAAIHGLVQAFLSAGD
                     HIVASQSLFGATVQLLGNILPRFGIEVTFVAATDLDAWRAAVQPNTKLFYLETPSNPL
                     TEIIDIRAVADIAHACGALLAVDNCFCSPALQKPLSLGADLVVHSATKYIDGQGRVLG
                     GAVVGSHELLEKLYLFVRSAGPTLSAFNAWVLLSGLETLFVRMERQCANALQLARWLE
                     THPAIEKVYYPGLPSHPQHELAMRQQTAGGAVVSFIVKGGREAAWKLIDSTQLVSITA
                     NLGDVKTTITHPATTTHGRVAPEVREKAGIVEGLVRVAVGLEHPADVQADLARGLEG"
     gene            complement(1965..2438)
                     /locus_tag="PKN55_09770"
     CDS             complement(1965..2438)
                     /locus_tag="PKN55_09770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013345880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4442 domain-containing protein"
                     /protein_id="HNX71574.1"
                     /translation="MAFTPSARTLKRLINLWPPFLGAGIRVLHISDDFRQAEVMLKLG
                     LSNRNYVGVHFGGSLYAMTDPFYMLLLMRSLGPDYVVWDKAGHIDYLQPGRGRVYARF
                     TLDDATLATIRAATAHGDKYLPELTVDVVDEHGQVVAQVRKTLYVRRKPGKPASA"
     gene            2758..3042
                     /gene="yajC"
                     /locus_tag="PKN55_09775"
     CDS             2758..3042
                     /gene="yajC"
                     /locus_tag="PKN55_09775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012698203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HNX71575.1"
                     /translation="MDQAALMQFLPMIGIFILFWFLMVRPQQKKMKEHKAMLDALQKG
                     DEVLTQGGIVGRITKAGAEYLSLEIANNVEIVVQRAAVGTKLEKGTLKSL"
     gene            3101..4921
                     /gene="secD"
                     /locus_tag="PKN55_09780"
     CDS             3101..4921
                     /gene="secD"
                     /locus_tag="PKN55_09780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012698204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HNX71576.1"
                     /translation="MNRYPLWKYLIIAVALIVSTLYTLPNFYGESPAVQVSTSRSAIA
                     IDTALMDRVEKSLAAAKLPADGIYLDGNSLKVRFHDPDTQIKARDLIQQELGDNYIIA
                     LNLLSSSPAWFAKLKAHPMFLGLDLRGGVHFLLEVDMKAAVDKTIERYSGDIRRELRA
                     KKIRYGTLKRVGDSLELGMRDAASLKAAEDVLARMLPNLTLKTDDTLNKLVISIKPEE
                     FVKIQTDAVKQNITTLHNRVNELGVAEPVIQQQGANRIVVQLPGVQDTAKAKDILGRT
                     ATLEVRMVEDDPAKVAEAQSGLVPSGYELLDEATGRGGVQNILVKKDVELTGDNINDA
                     QPGFDENGTAAVHINLDSAGASIFRQVTADNIGRRMAMILVEKGKAEVVTAPVIRSEI
                     GGGRVQISGSMNPAEANDVALLLRAGSLAAPMNIIEERTVGPSLGKDNIEKGFNSTLY
                     GFVAIAIFMVIYYRVFGMISAVSLAVNVLMLVALLSMLQATLTLPGIAAIALTLGMAI
                     DANVLINERVREEVRNGVTPQAAIKAGYEHAWATILDSNITTLIAGLALLIFGSGPVR
                     GFAVVHCLGILTSMFSAVMVSRGLVNLVYGSRRRVAKLSV"
     gene            4938..5873
                     /gene="secF"
                     /locus_tag="PKN55_09785"
     CDS             4938..5873
                     /gene="secF"
                     /locus_tag="PKN55_09785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HNX71577.1"
                     /translation="MELLHFKKDIPFMSYGKLTTAISLLTFLLSVFFLFSKGLHFSVE
                     FTGGTVMEVQYTQPVELNSVRSKLDGLKLGEATVQSLGTSRDVLIRLPNLKDRTSAQL
                     SNQVLAALQADNTQVELRKVEFVGPQVGEELVTSGLTALTLVCAGIMLYLAIRFEWRF
                     AVAAILANMHDVVIILGCFALFQWEFSLTVLAAVLAVLGYSVNESVVVFDRIRENFRK
                     SGMRGKTTAQVIDNAITATMSRTIITHGSTEAMVLSMLVFGGPALHGFATALTIGIVF
                     GIYSSVLVASPIALALGVKREHMIKPVKPKEEAVV"
BASE COUNT         1131 a         1841 c         1918 g         1154 t
ORIGIN      
        1 ccgcagccgg gcaggctggc gcgcagattc atcggcatct ggctgcgcag cgcggtaaag
       61 cccgaatgca tctcggcgct gaccgcccgc gccaaggcgc gctgacgggc gttgcccggc
      121 cacaggccaa gctcggggta gcgctcggcc aggtattcgc caatcgccag cgaatcccac
      181 accaccagct caccatcacg cagcactggc accttgccgc tgggcgacac cgcccgcagc
      241 ttgctggcgc tgtctgcctg atacagcgcc acccgcgact cgcggaacgg aatatcaaaa
      301 tggcgcatga ccagccaagg ccgcagcgac cacgacgagt aattcttgtt gccgatgtac
      361 agcgtgaaca tgacacaagc tcctgacaaa tcccggctgc gggcgattcc cgccattgcc
      421 acaagcatgc agactacacg cagggggtgg ggtggaacaa gttgagagaa gcttagcgcc
      481 gctcacaaaa cccctggcgt tgttgtctgt tccccgcatc agcggggatg aaccgtttca
      541 gtgcacctac gtcaagcagg tgtttgaccg ttccccgcac ccggtgcgca gcccaccagt
      601 atttactccc catctcgcac cggcataaaa aaagcggatt tgcctcacat ataagacaaa
      661 tccgcttctg tccccacgtt tcagtatccg gcccattcaa gggcttgggt tcagccttcc
      721 aggccgcgcg ccagatcagc ttgcacgtcg gcggggtgtt ccaggccgac ggccacgcgc
      781 accaggcctt cgacgatgcc ggctttttcc cgcacttccg gcgccacgcg gccgtgggtg
      841 gtggtggccg ggtgggtgat ggtggttttc acgtcgccca ggttggcggt gatcgacacc
      901 agttgggtgg agtcaatcag cttccaggcg gcttcgcgtc cgcccttgac gataaagctc
      961 accactgcgc cgccggcggt ttgctggcgc atggccagtt cgtgctgcgg gtggctgggc
     1021 aggccggggt agtacacttt ttcgatggcc gggtgggttt ccagccagcg ggccagttgc
     1081 agggcgttgg cgcattggcg ctccatgcgc acaaacaggg tttccaggcc ggagagcagc
     1141 acccaggcgt tgaaggccga cagggtgggg ccggcgctgc gcacaaacag atacagcttt
     1201 tccagcagct cgtggctgcc caccacggcg ccgcccagca cgcggccctg gccgtcgatg
     1261 tatttggtgg ccgagtgcac caccaggtcg gcgcccaggc tcagcggctt ttgcagcgct
     1321 ggcgagcaga agcagttgtc caccgccagc agcgcgccac aggcgtgggc gatgtcggcc
     1381 acggcgcgga tgtcgatgat ttcggtcagc gggttggacg gggtttccag gtaaaacagc
     1441 ttggtgttgg gctgcacggc ggcgcgccag gcgtccaggt cggtggcggc gacaaaggtc
     1501 acttcaatgc caaagcgcgg caggatattg cccagcagct gcacggtggc gccaaacagg
     1561 ctttgcgaag ccacaatatg gtcgccggca gacaaaaacg cctgcaccag accgtggatg
     1621 gcggccatgc cgctggaggt ggccacggcg cgctcgccgc cttccagcgc cgccagccgc
     1681 gcctgaaagg cgctgacggt ggggttggtg aagcgcgaat aggtgtagcc ggggatttcc
     1741 ccggaaaaca gcttggcggc ttcggccgcc gaggcggatt tgaagctgga ggtcaaaaac
     1801 atcgcctggc tgtgttcttg gtagtccgag gtttctttgc cggcgcggat ggccagggtt
     1861 tccgggtgca gcggggtggt gctcatggtg gggccttgtt cattccagtg tttggcgagc
     1921 ataccccgct gcggggcaat tccaaacatt ttttgggaat aagttcatgc gctggctggt
     1981 ttgcctggct tgcgccggac gtacagtgtc ttgcgcacct gcgccaccac ttggccgtgt
     2041 tcgtccacca cgtccaccgt cagttcgggc aggtatttgt cgccgtgggc ggtggcggcg
     2101 cggatggtgg ccagggtggc gtcgtccagg gtaaagcggg cgtatacccg gccacggccg
     2161 ggctggaggt agtcgatatg gccggctttg tcccacacca cataatccgg ccccagactg
     2221 cgcatcaata gcagcatgta aaatgggtcg gtcatggcgt acaggctgcc gccaaagtgc
     2281 acgcccacat agttgcggtt gctcaggccc agcttgagca tcacttcggc ctggcgaaag
     2341 tcgtcgctga tgtgcagcac gcggatgccg gcgcccaaaa acggcggcca caggttgatc
     2401 aggcgcttga gggtgcgggc ggagggggta aatgccatat caggattcgc aaacaaacgt
     2461 ttgaaaccgg cagattgccg ttcgtgtgcc acgccgtcaa cccgttggcc acgctggcga
     2521 cagaaaaacc acagcccatt cgtcgtctct gctgcgggaa tggcggtctg acgttgcccc
     2581 gggaacctcg gccatgcttt gtgggtcggt tacggctgca acatgacgat ggcggtggat
     2641 ttgccccccg catcacagct acaatgcccg gtcggactgc cgaccccggc ccgattttga
     2701 taacccggcg gccgccttaa ccctttgtgc cgcctttata aggtcgaaac agcaatcatg
     2761 gatcaagccg cgctcatgca atttctgccg atgatcggca ttttcattct gttctggttc
     2821 ctgatggtgc gcccgcaaca gaaaaagatg aaagaacaca aggcgatgct cgatgccctg
     2881 caaaagggtg acgaagtgtt gacccagggt ggcattgttg gccgcatcac caaggctggc
     2941 gccgaatatc tctctctgga aatcgccaac aacgtcgaga tcgtcgtcca gcgtgccgcc
     3001 gttggcacca agctggaaaa aggcacgctg aagtcgctgt aatttccgtt cacctcacgg
     3061 ccgccgcgcc cgcgcagcgg ccgcttccct gacgcgcctc atgaaccgtt atccgctctg
     3121 gaaatacctg atcatcgccg tggcgctgat cgtttccacc ctgtataccc tgcccaattt
     3181 ctacggcgaa agcccggcgg tgcaagtgtc cacctcccgc tcggccattg ccattgatac
     3241 cgcgctgatg gatcgggtgg aaaaatccct ggccgccgcc aagctgccgg ccgatggcat
     3301 ttatctggat ggcaatagcc tgaaagtgcg ctttcacgat ccggacaccc agatcaaggc
     3361 ccgcgatctg atccagcaag agctgggcga caactatatt attgcactga acctgctgtc
     3421 ctcgtcgccg gcctggttcg ccaagctcaa agcccacccg atgttcctcg gcctggattt
     3481 gcgcggtggc gtgcacttcc tgctggaagt ggacatgaaa gccgcggtgg ataaaaccat
     3541 cgagcgctac agcggcgaca tccgtcgtga gctgcgcgcc aagaaaatcc gctacggcac
     3601 gctcaagcgc gtgggcgaca gcctggagct gggcatgcgc gacgccgcct cgctgaaggc
     3661 cgccgaagat gtgctggcgc gcatgttgcc caacctcacc ctgaaaaccg acgacacgct
     3721 gaacaagctg gtgatcagca tcaagccgga agagttcgtc aaaatccaga ccgacgcggt
     3781 caagcagaac atcaccaccc tgcataaccg ggtgaacgag ctgggcgtgg ccgagccggt
     3841 gattcagcag caaggcgcca accgcattgt ggtgcagctg ccgggcgtgc aggacaccgc
     3901 caaggccaag gatattctgg gccgcaccgc cacgctggaa gtgcgcatgg tggaagacga
     3961 cccggccaag gtggccgagg cgcaatccgg cctggtgcct tctggctatg agctgctgga
     4021 cgaagccacc ggccgtggcg gcgtgcagaa cattctggtc aagaaagacg tcgagctgac
     4081 tggcgacaat atcaacgacg cccagcccgg ctttgacgaa aacggcaccg ccgccgtgca
     4141 catcaatctg gacagcgccg gcgccagcat cttccgtcag gtgactgccg acaatatcgg
     4201 ccgccgcatg gcgatgattc tggtggaaaa aggcaaggcc gaagtggtga ccgcgccggt
     4261 gatccgcagt gaaatcggcg gcggccgcgt gcagatctcc ggctcgatga acccggccga
     4321 agccaacgac gtggcgctgc tgctgcgcgc cggctcgctg gccgcgccga tgaacatcat
     4381 cgaagagcgc accgtcggcc cgagcctggg caaggacaat atcgaaaagg gctttaactc
     4441 caccctgtac ggttttgtcg ccatcgccat ctttatggtg atctactacc gcgtgttcgg
     4501 gatgatctcg gccgtgtcgc tggcggtgaa cgtgctgatg ctggtggcgc tgttgtccat
     4561 gctgcaagcc accctcaccc tgcccggcat cgccgccatt gccctgacgc tgggcatggc
     4621 gattgacgcc aacgtgctga tcaacgagcg ggtgcgcgaa gaagtgcgca acggcgtcac
     4681 cccgcaggcg gcgatcaagg ctggctacga acacgcctgg gcgaccattc tggattccaa
     4741 catcaccacc ctgattgccg gcctggccct gctgattttt ggctcgggcc cggtgcgcgg
     4801 ctttgccgtg gtgcactgcc tgggcattct cacctcgatg ttctcggcgg tgatggtgtc
     4861 gcgcggtttg gtcaatctgg tgtacggcag ccgccgccgc gtggccaaac tgtcggttta
     4921 acaagggaac agcacgaatg gaattgctgc atttcaaaaa agacatcccg ttcatgagct
     4981 atggcaagct caccacggcg atttccctgc tgaccttttt gctgtcggtg ttcttcctgt
     5041 tcagcaaggg cctgcatttc agcgtggaat tcactggcgg cacggtgatg gaagtccaat
     5101 acacccagcc ggtggagctc aacagcgtgc gcagcaagct ggacggcctc aagctgggcg
     5161 aagccaccgt gcaaagcctg ggcaccagcc gcgatgtgct gattcgcctg cccaatctga
     5221 aagaccgcac cagcgcccag ctgtccaatc aggtgctggc cgcgctgcaa gccgacaaca
     5281 cccaggtgga gctgcgcaag gtggagtttg tcggcccgca ggtgggcgaa gagctggtga
     5341 ccagcggcct gaccgcgctg accctggtgt gcgccggcat catgctgtat ctggccatcc
     5401 gctttgaatg gcgctttgcc gtggccgcca tcctggccaa tatgcacgat gtggtgatta
     5461 ttctgggctg ctttgccttg ttccagtggg agttctcgct gaccgtgctg gctgcggtgc
     5521 tggcggtgct gggctattcg gtgaacgaat cggtggtggt gtttgaccgg attcgcgaga
     5581 atttccgcaa atccggcatg cgcggcaaga ccacggcgca ggtgatcgac aacgccatta
     5641 ccgccaccat gagccgcacc atcatcaccc acggttcgac cgaagccatg gtgctgtcca
     5701 tgctggtgtt tggcggcccg gcgctgcatg gttttgccac tgcgctgacc atcggcattg
     5761 tgtttggcat ttattcgtcg gtgctggtgg ccagcccgat tgccctggcg ctgggggtga
     5821 agcgcgagca tatgatcaag ccggtgaagc cgaaggaaga agcggtggtg tgaggtttgg
     5881 ggtgtggcgg ggtgtagccg cgctggcgcg cgggtgtgct gggggtgatt aagacggggt
     5941 tgcaccccgt gcccgcccta ctttctttgc ttcgccaaag aaagtaggca aagaaaggcg
     6001 acccgggcgt cgcggtcagc cctgcgggct gactgccctg tgct
//
LOCUS       DAOPYX010000186         5903 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_16754_length_5903_cov_7.571990, whole genome shotgun sequence.
ACCESSION   DAOPYX010000186 DAOPYX010000000
VERSION     DAOPYX010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 5903)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5903)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5903
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16754_length_5903_cov_7.571990"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..326)
                     /locus_tag="PKN55_09790"
     CDS             complement(<1..326)
                     /locus_tag="PKN55_09790"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNX71578.1"
                     /translation="MSALLDSQALTLRADGRTLLNALDWRLEAGQCWAVIGRNGAGKS
                     TLLRTLAGLRAAEDGRIQLAGRALADWPLAARARQLGYLPQSWRDAFACQVLDALLIA
                     RHPHRQG"
     gene            complement(323..1339)
                     /locus_tag="PKN55_09795"
     CDS             complement(323..1339)
                     /locus_tag="PKN55_09795"
                     /inference="COORDINATES: protein motif:HMM:NF013219.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HNX71579.1"
                     /translation="MTLAARPPWRALWLLLALSLATVLGFALAAGLGSAQLRLDEAWA
                     ALSGRAAADSLASALLALRLERAVVAWHTGAALALSGVLMQALLRNPLADPYILGVSG
                     GAAVGALLALLLGLALWWVDAGALLGAAAVSALLLGLARRDWQGGPLGATLLLLTGVM
                     LSTACSALISLLLSLAPDSRLRPMVFWLIGDVSASRWQPTLSVLLLAALALCLRQARA
                     INVLAFYGDDAHTLGVPAARLRRVLFLTAAALTAGAVANAGSIGFIGLMIPHACRFAL
                     GSDHRVLLPAATLAGGLLLLLADVAARTVAAPMQLPVGVITALIGVPAFLWQLRQVRQ
                     GGRA"
     gene            complement(1336..3174)
                     /locus_tag="PKN55_09800"
     CDS             complement(1336..3174)
                     /locus_tag="PKN55_09800"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNX71580.1"
                     /translation="MNVFFPGRVRHLGALACLAWAMPSLAADALSEITVTAARQAQPR
                     QAALADVTIIDRAELDRAGQDALPQLLARERGVEVAGNGGIHNSTSVFIRGANSNQTL
                     VLVDGQRLGSATTGGAALDAVPLAAIERVEILRGPASSLYGADAIGGVINIITRQGED
                     GPAKLRASIGAGSDETVRTQAAVSGGGDGWRYAVSGGYGQSAGFSAIANTGSSQYNPD
                     RDGYAERNVSAQLSRQWQPGHSLDARYLYSRVNSEYDGSKTGYDRTRQIVSALSLASN
                     NQLTSWWHSRLRLGETLDDSTTYSQYPSQIRTRQRQYSWQNDFTLAASQRLSLALERL
                     EERVSSSGFAGKAPEGRNTNSATLVYAGQFGDHHVQANLRQDDNSAYGDKTTGGLSYG
                     YDLSRQWRATVSGSTGFRAPTFNELYYPGYGQTQIRPETSRNLEASLRYQDGGSQAGL
                     TVYRNRVHDLIVTQRPCSVKGYLSCANNVNRAVLEGVSLSLGHSWSSTRVRADLDWQN
                     PHDADTGKRLARRASRLAKFSVNQDWAGWDLGAEWQLSNRRYDNVANTNALGGYGLVN
                     LTASRALSRDWQVQARWNNVGNKHYEQAKDYGVSGSTLFVNLVYQP"
     regulatory      complement(3322..3542)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(3643..4023)
                     /locus_tag="PKN55_09805"
     CDS             complement(3643..4023)
                     /locus_tag="PKN55_09805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011135142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HNX71581.1"
                     /translation="MPLAYWCLLIAGLLPYAWALVAKLGVPYDNHRPREVLAHAQGHR
                     KRANWAQANAFESLPLFIAAVLVASLQHADAGKIDNAALLFVLARLAHGLFYIADLAR
                     PRSLAWLLGIGCVMYLFLLAGRLL"
     gene            complement(4134..>5903)
                     /locus_tag="PKN55_09810"
     CDS             complement(4134..>5903)
                     /locus_tag="PKN55_09810"
                     /inference="COORDINATES: protein motif:HMM:NF025214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNX71582.1"
                     /translation="QAASNLGLSLLNRKQYAQARSVLENALAQYPDDLHIQINLANAL
                     AQCGELAAAIDMHARVLARQPDDVHTLVNLASLEIRRHDYAQAQTYLDQALVLAPQHS
                     GALSAQADAWRFQHHFEPAIALLRTLGEREPQVAHHPRRLGSALRMLGRHAEAMAAYQ
                     RALDIEPGDADTYSNLLLALQYSPGYTSEQVFAWHRGFGERYEAPLPLPRHFPHSRQP
                     ERRLRVGWVSGDFNAHPVAYFVLPLWSALDRVQFESFAYDTSLTADSVTERLQAHVDH
                     WRRVRGLTHEELARQIAHDRIDILIDLSGHTGGHRLPTFARKPAPVQAAWLGYADTTG
                     LSRIDWRLSDPYGDPDGAEARYTERLWRLPEVFCCYQPMVRMPELRDSPDYAVQPTPA
                     LANGHITFGCCNNYSKITNEVIALWARLLHAAPQARLLLELAGISHEASRDELLARFA
                     VHGIDAGRLQLEDRHPAWQYKRYHQIDICLDPFPATGGTSTCDLLWMGVPLVTLTGER
                     FASRLGVSLLNAVGHAEWIAADADAYVDIALGLAAEVTALDAIRQEMRPRMEASPLMD
                     AERFAGHFADALRGMWRDWCAQG"
BASE COUNT          976 a         1973 c         2062 g          892 t
ORIGIN      
        1 ccctggcggt gcgggtggcg ggcaatcagc agcgcgtcca gcacctggca ggcaaaggcg
       61 tcgcgccagc tttgcggcaa atagcccagc tggcgcgcgc gcgcggccag cggccagtcg
      121 gccagcgccc gcccggccag ctggatgcgc ccgtcttcgg cggcgcgcag gccggccagg
      181 gtgcgcagca gggtggattt gccggcgccg ttgcggccaa tcaccgccca gcattggccg
      241 gcctccagcc gccagtccag cgcattgagc agggtgcggc cgtcggcgcg caacgtcagc
      301 gcctggctgt ccagcaaggc gctcatgccc ggcctccctg gcgcacctgg cgcaattgcc
      361 acaaaaacgc cggcacgcca atcaaggcgg tgatcacgcc caccggcagc tgcatcggcg
      421 cggccacggt gcgcgcggcc acgtcggcca gcagcaataa caagccgccg gccagggtgg
      481 cggccggcag cagcacgcgg tggtcgctgc ccagggcaaa gcggcaggcg tgaggaatca
      541 tcagaccaat aaaaccaatg ctcccggcat tggccaccgc gccagcggtc agggcggcgg
      601 cggtgagaaa caacacccgg cgcagccggg cggcgggcac gcccagggtg tgggcgtcgt
      661 cgccataaaa agccagcaca ttgatggcgc gcgcctggcg caggcacagc gccagcgccg
      721 ccagcagcag cacgctcaag gtcggctgcc agcggctggc cgacacatcg ccaatcagcc
      781 agaacaccat cggccgcagc cggctgtccg gcgccagcga cagcagcagg ctgatcagcg
      841 ccgagcaggc ggtggacagc atcaccccgg tcagcagcag caaggtggcg cccagcgggc
      901 cgccttgcca gtcgcgccgg gccaggccca gcaacagcgc cgacaccgcc gccgcgccca
      961 agagcgcgcc ggcatccacc caccacagcg ccaggcccag cagcagggcc agcaaggcgc
     1021 ccaccgccgc gccgccagac acgcccagaa tatacgggtc ggccagcggg ttgcgcagca
     1081 gcgcctgcat cagcacgcca gacagcgcca gcgccgcgcc ggtgtgccag gccaccacgg
     1141 cgcgctccag ccgcagcgcc agcaaggcgc tggccagcga atccgccgct gcccgcccgc
     1201 tgagcgccgc ccaggcttcg tccaggcgca gctgcgccga gcccaggccg gcggccaggg
     1261 caaagcccag cacggtggcc aggctcagcg ccagcaacag ccagagggcg cgccagggcg
     1321 ggcgggcggc caaggtcacg gctgatacac cagattgaca aacagcgtgc tgccgctcac
     1381 gccgtagtcc ttggcttgtt catagtgctt gttgccgaca ttgttccagc gcgcctgcac
     1441 ctgccaatcg cggctgagcg cgcggctggc ggtgaggttg accaggccat agccgcccag
     1501 ggcgttggtg ttggcgacat tgtcgtaacg gcggttgctc agctgccatt ccgcgcccag
     1561 gtcccagccg gcccaatcct gattgacgct gaacttggcc agccggctgg cgcggcgcgc
     1621 caggcgtttg ccggtgtcgg cgtcgtgcgg gttttgccaa tccaggtcgg cgcgcacgcg
     1681 ggtgctgctc cagctgtggc ccaggctgag gctgacgcct tccagcacgg cgcggttgac
     1741 gttattggcg caggacagat agcccttcac gctgcacggg cgctgggtga cgatcagatc
     1801 atgcacgcgg ttgcggtaca cggtcagccc ggcctggctg ccgccatcct gataacgcag
     1861 gctggcttcc agattgcgcg aggtttccgg gcggatttgc gtctggccgt agccggggta
     1921 gtacagctcg ttgaaggtgg gcgcgcgaaa cccggtgctg ccgctgaccg tggcgcgcca
     1981 ctggcggctg aggtcgtagc catagctcag gccgccagtg gttttgtcgc cgtaggccga
     2041 gttgtcgtcc tggcgcagat tggcctgcac atggtggtcg ccaaactggc cggcgtacac
     2101 cagggtggcc gagttggtgt tgcgcccttc cggggccttg ccggcaaagc ccgagctgct
     2161 gacgcgttct tccagccgct ccagcgccag gctcaggcgc tggctggcgg ccagggtgaa
     2221 gtcgttttgc cagctgtatt ggcgctggcg ggtgcggatt tgcgaggggt attgcgaata
     2281 ggtggtgctg tcgtccaggg tttcgcccag gcgcaggcgg ctgtgccacc agctggtcag
     2341 ctggttgttg ctggccaggc tcagcgccga gacaatctgg cgggtgcggt cgtagccggt
     2401 tttgctgccg tcgtattcgc tgttcacccg gctgtacaga tagcgcgcgt ccaggctgtg
     2461 gcccggctgc cactggcggc tgagctgggc agacacattg cgctcggcgt agccgtcgcg
     2521 gtcggggttg tactggctgc tgccggtgtt ggcgatggcg ctaaagccgg cgctctggcc
     2581 atagccgccg ctgacggcat agcgccagcc gtcgccgccg ccgctgactg ccgcttgcgt
     2641 gcgcacggtt tcatcgctgc cggcgccaat gctggcgcgc agcttggccg ggccgtcctc
     2701 accctggcgg gtgatgatat tgatcacccc gccaatggcg tcggcgccgt acaggctgct
     2761 ggccgggccg cgcaggattt ccacccgctc gatggccgcc agtggcacgg cgtccagcgc
     2821 ggcgccgccg gtggtggccg agcccaagcg ttggccgtcc accagcacca gggtctggtt
     2881 gctgttggcg ccgcgaataa acacgctggt gctgttgtgg atgccgccat tgccggcaac
     2941 ttccacgccg cgctcgcgcg ccagcagttg cggcagcgca tcctggccgg cgcgatccag
     3001 ctcggcgcgg tcgatgatgg tgacatcggc cagcgcggct tggcgcggct gcgcctgacg
     3061 cgcggcggtg acggtgattt cagacagggc gtcggcggcc aaagacggca tggcccacgc
     3121 cagacaagcc agcgcgccaa gatggcgcac ccggccgggg aagaagacat tcatgaggta
     3181 aaacctgcaa tgctgggaca tgcgaccccg catgtccggt cagacggaca aggcgccgca
     3241 caggcgcgcc ctggccgcgc ctgacccgaa gagccctgcg cacccagcgc gccgcccgcc
     3301 gcagcgcctc acaagacggc gaacatcgcc gcccttgccg tccggtatcc gggctggcgt
     3361 ccttccccgg acgagccttc ccacatcagg cgatgcagtg gcgtttcgtc cggctagtct
     3421 tgccccatca gggcaaaacg ggcgctaacc gttgcggggg cagcacaggc agccgcgcaa
     3481 aagcgcgaca gtgtttcccg gtcagtcgta tcgtggtgac gactggcccc gccttcggag
     3541 gtatatccgg caggctgggg cgccagcaag gcgggggagt gtagcacagc ggggcgatgg
     3601 ctggcgcggg ttttgcagtg cagcaaagca gcgggccgcg gattacagca agcggccggc
     3661 cagcaggaac aaatacatca cgcagccgat gcccaatagc caggccagcg agcgcggccg
     3721 agccaggtcg gcaatgtaaa acagcccgtg cgccaggcgc gccagcacaa acagcagcgc
     3781 ggcattgtcg atcttgccag cgtcggcgtg ttgcaggctg gccaccagca ccgccgcgat
     3841 aaacagcggc agcgattcaa aagcattggc ctgcgcccag ttggcgcgct tgcgatggcc
     3901 ttgcgcgtgc gccagcacct cgcgcgggcg gtggttgtcg taggggacgc ccagtttggc
     3961 gaccagcgcc cacgcgtagg gcaataggcc ggcaatcagc aggcaccagt aggcaagcgg
     4021 catgggcgat ctcctggagg gcggcgcatg atgggccgcg ctggctgact ataacaccac
     4081 gtcgccagcc agtgctttat cctgcacgcc gctgggtgct gccactgtga gcatcagccc
     4141 tgtgcacacc agtcccgcca catgccgcgc agtgcgtcag caaaatggcc ggcaaagcgc
     4201 tcggcgtcca tcagcgggct ggcctccatg cgcgggcgca tctcctggcg gatggcgtcc
     4261 agcgccgtca cctctgcagc caggccgagc gcaatatcca cataggcatc ggcatcggcg
     4321 gcaatccatt cggcgtggcc cacggcgttc agcaggctga cccccaggcg cgaggcaaaa
     4381 cgctcgccgg tcagcgtcac cagcgggacg cccatccaca acagatcaca ggtcgaggtg
     4441 ccgccggtgg ccgggaaagg gtccagacaa atatcaatct ggtgatagcg cttgtattgc
     4501 caggccgggt ggcggtcttc cagttgcagg cggccggcgt cgatgccatg gacggcaaag
     4561 cgcgccagca attcgtcgcg cgaggcttca tggctgatgc cggccagttc cagcagcagt
     4621 cgcgcctgcg gtgcggcgtg caacagccgt gcccacaagg caatcacttc attggtgatt
     4681 ttggaataat tattgcagca gccaaaagtg atatggccat tggccagcgc cggggtgggt
     4741 tgcacggcgt aatccgggct gtcgcgcagt tcgggcatgc gcaccatcgg ctggtagcag
     4801 caaaacacct cgggcaatcg ccacagtcgt tcggtgtagc gcgcctcagc gccgtccggg
     4861 tcgccatagg ggtcgctcaa gcgccagtca atgcggctca gcccggtggt gtcggcatag
     4921 cccagccagg cggcttgcac tggcgccggt ttgcgggcaa aggttggcag gcggtggccg
     4981 ccggtgtggc cagacaaatc aatcaggatg tcgatgcggt cgtgggcaat ttgccgggcc
     5041 agttcctcgt gggtcagtcc gcgcacccgc cgccagtggt caacgtgtgc ttgcaggcgc
     5101 tcggtgacgc tgtcggcagt caggctggtg tcgtaagcaa acgattcaaa ctgcacacgg
     5161 tccagcgccg accacagcgg cagaacaaag taggccaccg gatgggcgtt gaagtcgccg
     5221 gacacccagc ccacgcgcag ccggcgctcg ggctggcggc tgtgcggaaa gtggcgcggc
     5281 aagggcagcg gcgcttcgta gcgctcgcca aagccgcgat gccaggcaaa tacctgctca
     5341 gaggtgtagc ccgggctgta ttgcagcgcc agcaacaaat tgctgtaggt gtcagcatcg
     5401 cccggttcga tatccagcgc gcgctgatac gccgccatgg cctcggcgtg gcggcccagc
     5461 atgcgcaggg ccgagcccag tcggcgcgga tggtgggcca cctgcggctc gcgctcgccc
     5521 agcgtgcgca gcagggcaat ggccggctcg aagtgatgct gaaagcgcca ggcgtcggcc
     5581 tgcgccgaca aggcgccgct atgctgcggc gccagcacca gcgcctgatc gagataagtt
     5641 tgcgcctggg cgtagtcgtg ccggcggatt tccaggctgg ccaggttcac cagcgtgtgc
     5701 acatcgtccg gctggcgggc cagcacgcgg gcgtgcatgt cgatggcggc agccagctcg
     5761 ccgcattgcg ccagggcatt ggccaggttg atctggatat gcagatcgtc cgggtactgg
     5821 gccagggcgt tttccagcac gctgcgcgcc tgggcgtatt gcttgcggtt cagcaggctc
     5881 aggcccagat tgctggccgc ctg
//
LOCUS       DAOPYX010000187         5901 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_16763_length_5901_cov_7.100924, whole genome shotgun sequence.
ACCESSION   DAOPYX010000187 DAOPYX010000000
VERSION     DAOPYX010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 5901)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5901)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5901
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16763_length_5901_cov_7.100924"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..715)
                     /locus_tag="PKN55_09815"
     CDS             complement(<1..715)
                     /locus_tag="PKN55_09815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014086678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNX71583.1"
                     /translation="MNKPKLVMVGNGMAGVRALEELFKLAPDAYHVTVFGAEPHPNYN
                     RILLSPVLAGEQTLDEIILNPLSWYEEHGVTLHLNRKVTRIDRQRRVVIADDGTEAEY
                     DRLLLATGSNPFILPIPGKDLDGVIGYRDIHDTNSMIDAAARYRHAVVIGGGLLGLEA
                     ANGLKLRGMDVTVVHLSDWLLERQLDHTAGKLLQTSLEARGLKFLLPKHTAELLGGED
                     GRVKAVRFKDGEEIPADLVV"
     gene            complement(816..1610)
                     /locus_tag="PKN55_09820"
     CDS             complement(816..1610)
                     /locus_tag="PKN55_09820"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019923750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNX71584.1"
                     /translation="MDNRYVHLEQVGMAFDTKKGRFVALQNVNLDIAQGEFISLIGHS
                     GCGKSTVLNVIAGLLTPTSGVALAAGREIDGPGPERAVVFQNHSLLPWLSCFGNVYLA
                     VERVFGKKENKAQLKARTHAALEMVKMTHATDKLPNEISGGMKQRIGIARALAMEPKV
                     LLMDEPFGALDALTRAHLQDELMRIVQATGSTVVMVTHDVDEAVLLSDRIVMMTNGPA
                     ATVGEILTVNLARPRDRLALANDAEYHHYRSAVLEFLYHKELRPAA"
     gene            complement(1631..2527)
                     /gene="ntrB"
                     /locus_tag="PKN55_09825"
     CDS             complement(1631..2527)
                     /gene="ntrB"
                     /locus_tag="PKN55_09825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019923749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015414 - ABC-type nitrate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015706 - nitrate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate ABC transporter permease"
                     /protein_id="HNX71585.1"
                     /translation="MTQSATVTVDAKASAHSAAAPAAAKPAAAPAARPAAEASPGIDW
                     RELIGQRIIPPILGFALFIAVWAAVAQVTKGIPGPSSTFVAAVKLFSDPFYIAGPNDV
                     GIGWKILNSLSRVGLGFGLAALVGIPAGFLLGRFMFLNRMLSPVIGLLRPVSPLAWLP
                     IGLLVFKAAQPAAIWVIFISSIWPIILNTAAGVSTVPQDYLNVARVLKLSEWKVFTTI
                     LFPSVLPHLMTGIRLSIGVAWLVIVAAEMLTGGVGLGFWVWDEWNNLNVEHIIVAIFV
                     VGLIGLALEQGLMMLARRFQYN"
     gene            complement(2543..3808)
                     /locus_tag="PKN55_09830"
     CDS             complement(2543..3808)
                     /locus_tag="PKN55_09830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008953681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CmpA/NrtA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HNX71586.1"
                     /translation="MTDHLQAATPSRRDFLKTCAAGVSASAITTLIPAGVKSAAYAGG
                     SDAPEKAAVKIGFIPLTDCAPIVVAATQNFGKKYGLTITPSKEASWAAVRDKLVNGEL
                     DAAHVLYGLIYGVQMGIGGQQKNMATLMTLNQNGQAITLSNQLRDEKKVSDGASLKKL
                     ISAEPREYTFAQTFPTGTHAMWLYYWLASIGVNPLKDVKTIVVPPPQMVANMRIGNMD
                     GYCVGEPWNARAVADKIGFTVATSQDIWAGHPEKVLGATADFVKQYPNTARAMVASIL
                     EASRYIDAKANRASVAKLIADKAYVNAPEDVIVQRFMGNYDNGIGKRWQDPNLMKFYD
                     EGKVSFPYLSDGMWFLTQHKRWGLLKSEPDYLAIAKQVNQIELYTQAAAMAKVPVPKD
                     PMRSAKLIDGKVWDGKDPKGYANSFAIKA"
     gene            complement(4126..4701)
                     /locus_tag="PKN55_09835"
     CDS             complement(4126..4701)
                     /locus_tag="PKN55_09835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018150520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ANTAR domain-containing protein"
                     /protein_id="HNX71587.1"
                     /translation="MLKVLLVNDTPRHLGRLRDALTAAGCLVVGEADNAFILADQVAQ
                     LAPDVIIVDTESPSRDTLEQVCVLSQSAPHPIVMFTDDGGNSSIRAAIQAGVTAYVVE
                     GLAEARILPILQVAQARFEEEQRLRAQVSAAEARLAERKTIERAKGILMKSRGFSEAE
                     AYNVLRSKAMQTNCKLAEVAQRIIDMADLLG"
     gene            complement(4870..5670)
                     /gene="folE2"
                     /locus_tag="PKN55_09840"
     CDS             complement(4870..5670)
                     /gene="folE2"
                     /locus_tag="PKN55_09840"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018151503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003933 - GTP cyclohydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0035998 - 7,8-dihydroneopterin
                     3'-triphosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase FolE2"
                     /protein_id="HNX71588.1"
                     /translation="MTAELTAMPDVQASADTRNIAINKVGIKSIRHPVQVADRSGGVQ
                     HSVGMFDMYVHLPQHFKGTHMSRFVEILNAQERELSVASFEQIVRQMVERLEAPSGYL
                     EMRFPYFVNKTAPVSGVQSLLDYDVTFIGELRDGEYRFTMKVLIPVTSLCPCSKKISE
                     RGAHNQRSHITITARTAQHVWIEELIDIAERQASCELYGLLKRPDEKYVTERAYDNPK
                     FVEDLVRDVAAALTADARIDAYVVESENFESIHNHSAYALIEFERPQT"
BASE COUNT         1136 a         1779 c         1813 g         1173 t
ORIGIN      
        1 tcaccaccag gtcggcgggg atttcttcgc cgtctttaaa gcgcacggcc ttgacgcggc
       61 cgtcttcgcc gcccagcagc tcggcggtgt gtttgggcag caggaatttc aggccacggg
      121 cttccaatga ggtttgcagc agcttgccgg cggtgtggtc cagctggcgc tccagcagcc
      181 agtcactgag gtgcaccacg gtgacatcca tgccgcgcag cttcaggccg ttggcggcct
      241 ccaggcccag caggccgccg ccgatcacca cggcgtgacg atagcgcgcg gcggcgtcga
      301 tcatgctatt ggtgtcgtgg atgtcgcgat agccgatgac gccatcaagg tctttgccgg
      361 gaatcggcaa gatgaacggg ttcgagccag tggccaacag cagacggtcg tattccgcct
      421 cggtgccatc gtcggcgatc accacccggc gctggcggtc gatgcgggtc accttgcgat
      481 tcagatgcag ggtgacgccg tgttcttcgt accaggacaa cgggttgagg atgatttcgt
      541 cgagggtctg ttcgccagcc agcaccggcg agagcagaat gcggttgtag ttgggatgcg
      601 gctcggcgcc gaacacggtg acgtggtaag catcgggcgc cagcttgaac agctcttcca
      661 gggcgcgcac cccggccatg ccattgccga ccatcaccag tttgggtttg ttcatcttgt
      721 ccaccttctc ggctgtggat ggcgggcaag gcttgcccct gcgggccggc ttttgccggt
      781 tcgccatcca gatagcatcg ggttagttca ggaaatcagg ctgccggacg cagctctttg
      841 tgatagagga attccagcac cgccgaacgg tagtggtggt attccgcatc attggccagt
      901 gccaggcggt cgcgcggacg cgccaggttc acggtcagaa tctcgccgac ggtggcggcc
      961 gggccattgg tcatcatcac gatgcggtcc gacagcagca cggcttcgtc cacatcgtgg
     1021 gtgaccatca ccacggtcga gccggtggcc tgcacgatgc gcatcagctc atcctgcaag
     1081 tgggcgcggg tcagcgcgtc cagggcgcca aacggctcgt ccatcagcaa gactttcggc
     1141 tccatggcca gcgcgcgggc aatgccgatg cgctgcttca tgccgccaga aatctcattg
     1201 ggcagtttgt cggtggcgtg ggtcattttc accatctcca gcgcggcgtg ggtgcgcgcc
     1261 ttgagctggg ctttgttttc ttttttgcca aacacccgct ccaccgccag gtagacattg
     1321 ccaaagcagc tcagccacgg cagcagcgaa tggttctgga acaccaccgc gcgctccggc
     1381 cccggaccgt cgatttcgcg gccggcggcc agcgccaccc ccgaggtcgg ggtcagcagg
     1441 ccggcaatca cgttcagcac ggtggatttg ccacagccgg agtggccaat cagcgagatg
     1501 aactcccctt gggcaatgtc cagattgacg ttctgcaggg cgacaaagcg gccttttttg
     1561 gtgtcgaacg ccatgccgac ttgttccagg tgcacgtatc ggttatccat tttccctctc
     1621 cctgcgcaaa tcagttgtac tggaagcgac gcgccagcat catcaagcct tgctccagcg
     1681 ccaggccaat caggcccacc acgaagatgg cgacaatgat gtgctccaca ttcaggttgt
     1741 tccattcatc ccacacccag aagcccaggc ccacgccgcc agtcagcatt tcggcagcga
     1801 caatcaccag ccaggccacg ccaatcgaca gacggatgcc ggtcatcagg tgcggcagca
     1861 ccgagggaaa cagaatggtg gtgaacactt tccattccga cagcttgagc acacgggcca
     1921 cgttcaggta atcctgcggc acggtggaca cgcctgcggc ggtgttcaaa ataatcggcc
     1981 agatgctgga gataaaaatc acccagatcg ccgccggttg cgcggccttg aacaccagca
     2041 gaccaatcgg cagccaggcc agcgggctga ccggacgcaa caggccaatc accggcgaca
     2101 gcatgcggtt caggaacatg aagcggccca acaagaaccc ggccggaatg cccaccagcg
     2161 ccgccaggcc aaagcccagg cccacccgcg acagcgagtt gagaatcttc cagccaatgc
     2221 cgacatcgtt ggggccggca atgtagaacg gatcagagaa caatttcact gccgccacaa
     2281 acgtcgagga cgggccagga atgcccttgg tgacttgcgc caccgccgcc cacacggcga
     2341 taaacaaggc aaaacccaaa atcggcggga tgatgcgttg gccgatcagc tcgcgccaat
     2401 caatgccagg gctggcctcg gctgccgggc gggcggcggg ggctgctgcg ggtttggccg
     2461 ccgccggggc ggcggccgag tgtgcagacg ccttggcgtc tacggtcaca gtggcggatt
     2521 gagtcatcgc gcacccctcc tatcaagctt tgatggcaaa gctgttggcg tagcccttcg
     2581 ggtccttgcc gtcccacacc ttgccgtcaa tcagcttggc cgaacgcatc ggatccttgg
     2641 gcaccggcac cttggccatg gcggcggcct gggtgtacag ctcgatctgg ttcacttgct
     2701 tggcgatggc caggtaatcc ggctcgctct tgagcaggcc ccaacgcttg tgctgggtga
     2761 ggaaccacat accatcggac aagtacggga aggacacctt gccttcgtcg tagaacttca
     2821 tcaggttcgg gtcttgccag cgtttgccaa tgccgttgtc gtagttgccc ataaagcgct
     2881 gaacaatcac atcttccggc gcgttcacat aagccttgtc ggcaatcagc ttggccacgc
     2941 tggcgcggtt ggccttggcg tcgatatagc ggctggcttc caggatggag gccaccatgg
     3001 cgcgggcggt gttcgggtat tgcttgacga agtcggcggt ggcgcccagc actttttccg
     3061 ggtggccggc ccagatgtcc tggctggtgg ccacggtaaa gccaattttg tccgccacgg
     3121 cgcgggcgtt ccacggttcg cccacacagt agccatccat attgccaatg cgcatattgg
     3181 ccaccatttg cggcggcggc accacgatgg tcttgacgtc cttgagcggg ttcacgccga
     3241 tgctggccag ccagtagtac agccacatgg cgtgggtgcc ggtcgggaag gtctgggcaa
     3301 aggtgtattc gcgcggttcg gcagagatca gctttttcag gctggcgccg tccgagactt
     3361 tcttttcgtc gcgcagttgg ttggacaggg tgatggcctg gccattctgg ttcagcgtca
     3421 tcagcgtggc catgttcttt tgctggccgc cgatgcccat ttgcacacca tagatcaagc
     3481 cgtacagcac atgagcggcg tccaattcgc cattcaccag cttatcgcgc accgccgccc
     3541 acgaggcttc tttgctcggg gtgatggtca ggccgtattt cttgccgaaa ttctgcgtgg
     3601 cagccaccac aatcggggcg caatcggtca gcggaataaa accgatttta accgcagctt
     3661 tttctggggc gtccgaaccg ccggcatatg ccgcgctctt cacgcccgcc ggaatcagtg
     3721 tggtaatggc cgacgccgac accccggcgg cgcaggtttt caggaaatca cggcggctgg
     3781 gggtggccgc ttgcaggtgg tcagtcatgg tggggcgctc ctgggttatg caataaaaat
     3841 tttgggcaat aaaaaaggcg tccatcaagc acgcagcttt cgccacgtct tgagggacgc
     3901 cattgtcctt gggccagact cgccgccatt ggcaagccga cctttacagc aaattcgggt
     3961 gcgacatcgg gtgtcgcgtt gcaccgccgt tggtgctcaa aacactataa gcagatagcg
     4021 tgccagtttt ttatatgtca gaaagccagt gttgggccgt ctatctgcgc tgtttgccgg
     4081 cttggtgcgc accatcctgg tgtgcagcgc accaaaaacg ggcgttcagc ccagcagatc
     4141 ggccatgtcg atgatgcgct gggccacctc agccagcttg cagttggtct gcatggcctt
     4201 gctgcgcaac acattgtagg cctcggcttc ggaaaacccc cggcttttca tcaggatgcc
     4261 cttggcgcgt tcgatggtct tgcgctcggc cagccgggct tcggctgcgc tcacctgagc
     4321 gcgcagtcgc tgttcttctt caaagcgcgc ctgtgccact tgcaggatgg gcagaatgcg
     4381 cgcctcggcc aggccttcca ccacataggc ggtcacgccg gcttggatcg ccgcccgaat
     4441 gctgctgttg ccaccatcat cggtaaacat cacgatcggg tgcggcgccg actggctgag
     4501 cacgcacacc tgctccagcg tatcgcgact gggtgattcg gtatcgacaa tgatcacatc
     4561 cggggcaagc tgcgcaacct gatccgccag aataaaagca ttgtcagctt cacccaccac
     4621 cagacagccg gcggcggtca gcgcatcgcg cagccgcccc agatgtcggg gagtatcgtt
     4681 gaccaacaaa actttcagca tgggtttctc gaaagatgcg gataaaacag tcatgcgcga
     4741 cgtccagcaa gaaccgtgca cgcgatgtgt ctgtctggca ggcggaggca cacaagcaag
     4801 caaaaaggcc atgggcagac cacgcggcaa gcgcagtctg cccatggccg ggcaggcaga
     4861 ccatcaaact caggtctggg ggcgctcgaa ctcgatcagc gcataagcag aatgattgtg
     4921 gatggattca aagttttcgc tttccaccac ataggcgtcg atgcgggcat cagcggttag
     4981 cgcggcagcc acatcgcgca ccagatcctc gacaaacttg gggttatcgt aggcgcgctc
     5041 ggtgacgtat ttttcatccg ggcgcttgag caggccatac agctcacagg aggcctggcg
     5101 ttcagcgatg tcgatcagct cttcgatcca cacgtgctgg gcggtgcgcg cggtgatggt
     5161 gatgtgcgag cgctggttgt gcgcgccgcg ctcggagatt tttttcgagc atgggcacag
     5221 gctggtgacc ggaatcaaca ccttcatggt gaagcggtat tcgccatcac gcagttcacc
     5281 gataaaggtg acgtcgtaat ccagcaggct ttgcacgccg gagactggcg cagttttgtt
     5341 gacgaagtac ggaaagcgca tttccagata gccggacggc gcttccagtc gctcgaccat
     5401 ctgccgcacg atctgctcga acgaggccac cgacagctca cgctcctgcg cgttgaggat
     5461 ttcgacaaaa cgcgacatat gggtgccctt gaaatgctgg ggcagatgca cgtacatgtc
     5521 gaacatcccc acactgtgct gcacaccgcc gctgcggtcg gccacctgca ccggatggcg
     5581 gatggatttg atccccacct tgttgatggc gatattgcgg gtgtcggccg aggcctgcac
     5641 gtcgggcatg gcggtaagtt cagcggtcat gggggcatga gaataaaaac aagaccgaac
     5701 gagtatagcg ccgctgaata gttgagtaaa gaactcggtt tttgcctggc tgcgtgggcc
     5761 ttggcatgac tcacaaaact caccgggcct tgctcgcgac cttgccgtgt aacaaggcca
     5821 ggcgggattg aaggagggga gtggggctta tccgctggct tctgtcaccg tgcgccaggc
     5881 ctgctccggc gcatgaatgc t
//
LOCUS       DAOPYX010000188         5899 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_16771_length_5899_cov_9.560575, whole genome shotgun sequence.
ACCESSION   DAOPYX010000188 DAOPYX010000000
VERSION     DAOPYX010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 5899)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5899)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5899
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16771_length_5899_cov_9.560575"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            227..1396
                     /locus_tag="PKN55_09845"
     CDS             227..1396
                     /locus_tag="PKN55_09845"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008952376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase family protein"
                     /protein_id="HNX71589.1"
                     /translation="MTEHAFSPLIIKGRTLLPIVQGGMGVGVSAHRLAGAVAKEGGVG
                     TIASVDLRHLHADLVEESAKFTEAADYDRLNLIALDREVKAALDIAQGHGMVSVNVMK
                     AVDSHAAYVRQACESGAQAITMGAGLPLDLPEMVQGYPDVALLPILSESRGIGIVIKR
                     WMKKNRLPDAIVIEHPKYAGGHLGAAKKEDIDNDRFEFKRVLDETFELFKTLGLEREK
                     IPLIVAGGVNSYEKVKTYLGWGAAGVQVGTAFAVTHEGDAHPNFKKVLADAKPEDIVE
                     FMSVAGLPARGVMTPFLKSYLKRESILQANAKADPKRCTQGLDCLSVCGLRDGLSKVG
                     QFCIDLKLSSAFRGEVSKGLFFRGSESLPFGHAIRSARELIDYLLTGVKPANLAA"
     gene            1504..2856
                     /locus_tag="PKN55_09850"
     CDS             1504..2856
                     /locus_tag="PKN55_09850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008952375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNX71590.1"
                     /translation="MTFSSCNPATGVGFYSRPAWTPAQLDASIAALRAVQPAWRRLPV
                     AERGHALRALSQQLLAHADELAGLISSEVGKLLAECRAEVEKSAKLCHYYADLAPELL
                     ATQEIPTLASRSGVSFEPLGLVLAVMPWNYPVWQVLRFAVPALAAGNACLVKPAPSVP
                     LTTERLLALVRAAGIDALDVAWIDTPQVEAAIAASDAVAFTGSTHTGRRIAELAGRHI
                     KKSVLELGGSNPCIVLADADIALAAEEAAHSRFRDAGQSCNAAKRMVLAPEIAEDFLQ
                     AFLDVARRLQSGDPRDPATTLAPLARADLRDALHQQVIDARAHGARCLLGGELPDGPG
                     FFYPATVLDQISPACRVWREETFGPVASVLRANSDLDAIRLANDTPFGLGASIYSADV
                     ERAQALAAELEVGSVFINRHTSSDLRLPFGGVKASGYGRELSEFGLYEFVNVKTYWQR
                     "
     gene            complement(2890..4509)
                     /locus_tag="PKN55_09855"
     CDS             complement(2890..4509)
                     /locus_tag="PKN55_09855"
                     /inference="COORDINATES: protein motif:HMM:NF024140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNX71591.1"
                     /translation="MNALKVSTRLYLLTGVLLAVSVAIALLGLRGMEQVVAGLDSVYK
                     DRVVPLRDIKLISDAYLALPGRLVGQVESREQSGAQAAQALTQQLKLARQHWHDYLAT
                     YLVDDEQREIAKLKPLLAEADRQLDGLAVVLNQQNPAALADFPHQDLERTRNAMQAPL
                     DRLVAVQLEVAKAEYDNSLALYHASQRNSLILLALSLLLGVGFVLWMARTLGRQLGAE
                     PQEVAEVAARIAAGDLRTPIACQTPGSVMQAMQKMQTSLQHLLGQIQSGAEHLASAAA
                     SLAATVEQVAGSTSAQSESTASIAAAVEELTTSIGHISSSAQQAAHTASAAGALSQES
                     GQATQAAISEVNNITTAVHHSAGALAELDGKSREISQVVNVIREIADQTNLLALNAAI
                     EAARAGESGRGFAVVADEVRKLAERTAASTGEISAMVNAIQADVEHARQRMSDGVSQV
                     ESGVERVATVGEVMHRLGGGIDEALEAIRDIANSLTEQGNASNAVAANVEKVAQMTEE
                     TSAAGHTLRDAAEGLNQLAASLRATVGRFQL"
     gene            complement(5048..>5899)
                     /locus_tag="PKN55_09860"
     CDS             complement(5048..>5899)
                     /locus_tag="PKN55_09860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012696509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HNX71592.1"
                     /translation="FKGSENLGNGLKAIWQVEQALSIDGTNVNGNNTWATRDSFVGLT
                     GNFGTVRLGKLSTYLNSDMEKFDAWIYGAGVNGATVTTANTLDGRLNNAIRYDSPNLN
                     GFKFTAAYGFDEARTTINNGNRSNKAVWNLGAGYENAGYFVDAGYVAFQDTDASGSKD
                     GNYWRLEGGYNANNLLVALAYGQSKLYAGAADATKAKEAALTVGYTMGALTPKFTYAH
                     VWDTKTNGVKADDGINQYVIGVDYALSKRTTAYTSFGYVKHDNVIVAGTDERKERTLA
                     VGVQHKF"
BASE COUNT         1004 a         1842 c         1892 g         1161 t
ORIGIN      
        1 gttcttgtaa agtgtgacgc tctccggggc agtttccggc attctcctgc aaatactggc
       61 tgagccgtgc cccggctgcc agatattgca gatcaacaaa acaatcgaac gattgagact
      121 tccgcccggg ctaaccttat aattcgacca tgcagttgac ccgcgtcaac gcgcccgcgg
      181 ccggcagcgg ccacacttgc gacttccttt ttgagccaga cccaccatga ccgagcatgc
      241 tttttctccc ctgatcatca aaggccgcac ccttttgccc attgtccaag gcggcatggg
      301 cgtgggcgtg tcggcgcacc ggctggctgg cgcggtggcc aaggaggggg gagtgggcac
      361 tatcgccagt gtcgatttgc gccatttgca cgccgatctg gtggaagaat cagccaagtt
      421 caccgaggcg gccgattacg accgcctgaa cctgattgcg ctggatcgtg aagtcaaggc
      481 tgcgctggac atcgcccaag gtcatggcat ggtgtcggtg aacgtgatga aggccgtgga
      541 ttcccacgcg gcctatgtgc gccaggcctg cgagtcgggt gcgcaagcca tcaccatggg
      601 cgctggcctg ccgctggacc tgccggaaat ggtgcagggt tatcccgatg tcgccctgct
      661 gccgattttg tccgagtcgc gcggcatcgg cattgtcatc aagcgctgga tgaagaaaaa
      721 ccgcctgccc gacgccatcg tgatcgagca ccccaagtac gctggcggcc acctgggcgc
      781 ggcgaaaaaa gaagacatcg acaacgaccg ctttgagttc aagcgcgtgc tggacgaaac
      841 cttcgagctg ttcaaaaccc tgggcctgga gcgggaaaaa attccgctga tcgtcgctgg
      901 cggggtcaac agctatgaaa aagtcaaaac ctatctgggc tggggcgctg ccggcgtgca
      961 ggtgggcacc gcgtttgccg tcacccacga gggcgacgcc caccccaact tcaaaaaagt
     1021 gctggccgac gccaagccgg aagacatcgt cgaattcatg agcgtggccg gcctgccggc
     1081 gcgcggggtg atgacaccgt tcctgaagag ctacctcaag cgcgaaagca ttctgcaagc
     1141 caacgccaag gccgacccca agcgctgcac ccagggcctg gactgcctga gcgtgtgcgg
     1201 cctgcgcgat ggcctgtcca aggtgggtca gttctgcatc gacctgaaac tgtcctccgc
     1261 cttccgtggc gaagtcagca agggcctgtt cttccgtggc tcggaaagcc tgccgtttgg
     1321 ccacgccatc cgcagcgcgc gcgaactgat cgactacctg ctgactggcg tcaagccggc
     1381 caatctggct gcgtaaaccc cgccatctcg cccatccgac cgtgaccgcc gcctgatgcg
     1441 cagacttgtt taatttgtta aacatgctgc gcatcaggcg gctttgtttt atgctttccg
     1501 accatgacct tttccagctg caacccggcc accggcgtcg ggttttattc ccgcccggcg
     1561 tggacgccgg cgcaacttga cgccagcatt gccgccctgc gcgccgttca gcccgcctgg
     1621 cgacgcttgc cagtggccga acgcggccac gcgctgcgcg cgctcagcca gcaattgctg
     1681 gcccacgccg acgaactggc cgggctgatt tccagcgaag tgggcaagct gttggccgag
     1741 tgccgcgccg aggtggaaaa atccgccaag ctgtgccact actacgccga cctggccccc
     1801 gagctgctgg ccacgcagga aatccccacc ctggccagcc gttctggcgt cagctttgaa
     1861 cccttgggcc tggtgctggc ggtgatgccg tggaattacc cggtgtggca ggtgctgcgc
     1921 tttgccgtgc cggcgctggc cgccggcaat gcctgtttgg tcaagccggc gccgtcggtg
     1981 ccgctgacca ccgagcgttt gctggcgctg gtgcgcgccg ccggcatcga cgcgctggac
     2041 gtggcctgga tcgacacccc gcaggtggag gccgccattg ccgcctcgga tgccgtcgcg
     2101 ttcaccggct cgacccacac cggccggcgc attgccgagc tggccggccg gcacatcaag
     2161 aaaagcgtgc tggagctggg cggcagcaac ccctgcatcg tgctggccga cgccgatatt
     2221 gccctggccg ccgaagaagc cgcgcattcg cgctttcggg acgccggcca gtcgtgcaac
     2281 gccgccaagc gcatggtgct ggcccctgag attgccgagg attttttgca ggcgtttttg
     2341 gatgtcgccc gccgcttgca gtctggcgac ccgcgcgacc cggccaccac gctggcgccg
     2401 ctggcccgcg ccgacctgcg cgacgccctg caccaacaag tgatcgacgc ccgcgcccac
     2461 ggcgcgcgct gcctgctggg cggcgaactg cccgacgggc cggggttttt ctacccggcc
     2521 accgtgctgg atcagatcag ccccgcctgc cgggtgtggc gcgaagaaac ctttggcccg
     2581 gtggccagcg tgctgcgcgc caattcagac ctggacgcca tccgcctggc caatgacacg
     2641 ccgtttggcc tgggcgccag catttacagc gccgacgtcg aacgcgccca ggcgctggcc
     2701 gccgagctgg aagtcggcag tgtgttcatc aaccgccaca ccagctcgga cttgcgcctg
     2761 ccgtttggcg gggtaaaggc ctcgggctat ggccgcgagc tgtccgagtt tggcttgtat
     2821 gaatttgtca atgtcaaaac ctactggcag cgttgaagcg ccgcgcggcg cgtgctgcgc
     2881 cgctgtgctt catagctgaa agcggcccac ggtggcgcgc aggctggcgg ccagttggtt
     2941 cagcccttcg gcggcgtcgc gcagggtgtg gccggcggcg gaggtttctt cggtcatttg
     3001 cgcgactttt tccacattgg ccgccacggc gttgctggcg ttgccttgtt cggtcagcga
     3061 gttggcgatg tcgcggatcg cctccagcgc ttcgtcgatg ccgccgccca gcctgtgcat
     3121 cacctcgccc accgtggcga cgcgctcgac gccgctttcc acttggctga cgccgtcgct
     3181 catgcgctgt ctggcgtgtt ccacatcggc ctgaatggcg ttgaccatgg cgctgatttc
     3241 gccagtggag gcggcggtgc gttcggccag cttgcgcacc tcatcggcca ccacggcaaa
     3301 gccacggcca gactcaccgg cgcgggccgc ttcaatggcg gcgttcaagg ccaagagatt
     3361 ggtctggtcg gcaatctcgc ggatgacgtt caccacttgc gagatttccc gtgatttgcc
     3421 gtccagctcg gccaaggcgc cggcgctatg gtgcacggcg gtggtgatat tgtttacctc
     3481 gctgatggct gcctgggtgg cctggccgct ttcctggctg agtgcgcccg ctgcgctggc
     3541 ggtgtgcgcg gcctgctggg cgctgctgga aatatggccg atgctggtgg tgagctcttc
     3601 gaccgcggcg gcaatcgagg cggtggattc gctttgtgcg ctggtgctgc cggccacttg
     3661 ttcaacggtg gcggccaggc tggcggcggc gctggccagg tgttcggcgc cggactggat
     3721 ttggccgagc aggtgttgca ggctggtttg cattttctgc atggcctgca tcacgctgcc
     3781 cggcgtctga caggcaatcg gcgtgcgcag gtcgccggcg gcaatgcgcg cggccacctc
     3841 ggccacttcc tgcggctcgg cgcccagttg ccggcccaag gtacgcgcca tccacagcac
     3901 aaagcccacg cccaagagca gcgacagcgc cagcaggatc aggctgttgc gctggctggc
     3961 gtgatacaag gccagcgagt tgtcgtactc ggctttggcc acttccagtt gcactgccac
     4021 cagtcgatcc agcggggctt gcatggcgtt gcgggtgcgc tccaggtcct gatgcggaaa
     4081 atcggccaag gcggccgggt tttgctggtt cagcaccacc gccaagccat ccagctgacg
     4141 gtcagcttcg gccagcagcg gtttgagctt ggcgatttcg cgctgctcgt cgtccaccag
     4201 ataggtggcc aggtagtcat gccagtgctg gcgggccagc ttgagttgct gggtgagcgc
     4261 ttgcgccgcc tgggcgccgg attgttcacg gctttccact tggccgacca gccggccggg
     4321 cagggccaga taggcatcag aaatcagctt gatgtcgcgc aagggcacca cccggtcttt
     4381 gtacacgctg tccagcccgg ccaccacctg ctccatgccg cgcagtccca gaagcgcgat
     4441 ggcgaccgac accgccagca gcacgccagt gagcagatac aagcgggtgg aaactttcag
     4501 ggcgttcatg gtgagtcttt cgtttcgatg acacttcaca aaattgacat gtgacagagg
     4561 cagtgccagt acagggggct gcgacgaaat gccacattgg caaatcctgc gcagggttct
     4621 aaagtaagtc ccgaatgctc gcgactccaa gcgcttgccg actcgcgcga aacacgcccc
     4681 ctgttttgtg cccaatggcc cacgatgttg atcgtgggcc attgtgtttt ctggggaggg
     4741 cgcattccgc ccagcaggac ggacgggacg ctcacgctac ggcagccgct ggcgtgggtc
     4801 aatacgctaa atagcgtacc tgcttgtttt tagtaaaata tggcgggttt atgatcatgg
     4861 cgagcatgaa ttttgtttgg atcgcccatc actgcgcgca agcggcaaag ggatcactga
     4921 caaaagaata ggcgagaaca cgccggagtg acacaacagg atagcccaag cggacagcag
     4981 cggatgacag acaataaaaa aaggtggccg aagccacctt ttttgttcga ttcaggaaaa
     5041 tcccgaatta gaacttgtgt tgcacgccca cggccagggt gcgttccttg cgctcgtcgg
     5101 tgccggcaac gatcacgttg tcgtgcttga catagccgaa ggaggtgtaa gcggtggtgc
     5161 gcttggacag ggcgtagtcc acaccgatca cgtattggtt gatgccgtcg tcagccttca
     5221 cgccgttggt cttggtgtcc cacacgtgag cgtaggtgaa cttcggggtc agggcgccca
     5281 tggtgtaacc cacggtcagg gcggcttcct tggccttggt ggcgtcagca gccccagcgt
     5341 acagcttgga ttggccgtag gccagagcca ccagcaggtt gttggcgttg tagccacctt
     5401 ccagacgcca gtagttgccg tccttggagc cggaagcgtc ggtgtcttgg aaagccacgt
     5461 agccagcgtc aacgaagtag ccagcgtttt cgtagccagc gcccaggttc catactgcct
     5521 tgttagaacg gttgccgttg ttgatggtgg tgcgggcttc gtcaaagccg taggcagcgg
     5581 tgaacttgaa gccgttcagg ttcgggctgt cgtagcggat ggcgttgttc agacggccgt
     5641 ccagggtgtt ggcggtggtc acggtagcac cgttcacgcc agcgccgtag atccaggcgt
     5701 caaacttttc catgtcggag ttcaggtagg tgctcagctt acccagacgc acagtaccga
     5761 agttgccggt caggcccacg aagctgtcgc gggtagccca ggtgttgttg ccgttgacgt
     5821 tggtgccgtc gatggacagg gcttgctcaa cttgccagat agccttcagg ccgttgccca
     5881 ggttttcgct gcccttgaa
//
LOCUS       DAOPYX010000189         5897 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_16789_length_5897_cov_15.015063, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000189 DAOPYX010000000
VERSION     DAOPYX010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 5897)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5897)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5897
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16789_length_5897_cov_15.015063"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..509)
                     /locus_tag="PKN55_09865"
     CDS             complement(<1..509)
                     /locus_tag="PKN55_09865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011133705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HNX71593.1"
                     /translation="MAWPDALPRRGLYLITPDTTDSLRLFSQCLRVLPARPALLQYRN
                     KSTDPALRLAQAQTLRALTRAAGVGLIINDDVALAAQVGADGVHLGRDDGDIAAARAR
                     LGEHAILGASCYDQLPLAHAAIAAGADYVAFGAVCPSATKPNAVRAPLSLFADSAALG
                     APRVAIGGIT"
     gene            complement(496..1365)
                     /locus_tag="PKN55_09870"
     CDS             complement(496..1365)
                     /locus_tag="PKN55_09870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018746268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HNX71594.1"
                     /translation="MSQIAEFPPIVMTLAGADPSGGAGIQADILTLASLGCHPLSVIT
                     AVTVQDTRSVENFWVMDAEQVSDQARFLLEDMPVSVFKVGMIGSVENAAVIAEIAADY
                     PDVPLILDPVLASGGGDELAREELCEAIRDLLLPLTTLVTPNSIEARRLAASNPDEED
                     ELSLPQAAQRLLQAGVEYVLITGTHDNTVQVCNTLYHRSGMTSREHWPRLPGSYHGSG
                     CTLASAIAGSLANGLSMPDAVREAQAYTWETLNRAFRPGMGQAIPDRLFWARGDATAE
                     DEAPAAGEPDGLA"
     gene            1433..1597
                     /locus_tag="PKN55_09875"
     CDS             1433..1597
                     /locus_tag="PKN55_09875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HNX71595.1"
                     /translation="MKTWMCLICGFIYDEAQGRPEDGIAPGTRWEDVPMNWTCPDCGA
                     RKDDFEMVEF"
     gene            1619..2035
                     /locus_tag="PKN55_09880"
     CDS             1619..2035
                     /locus_tag="PKN55_09880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HNX71596.1"
                     /translation="MDYLIIKALHLFFVIAWFAGLFYLPRIFVNLAMAGDMVELARLK
                     LMAGKLYRFMTPLGLIALALGLVLWLGYGVSGGWLHAKLTLVAGLIVYHLMCRHFLQV
                     FIADANTRSHTWFRVFNEIPVLMLLAVVLLVIVKPF"
     gene            2065..2556
                     /locus_tag="PKN55_09885"
     CDS             2065..2556
                     /locus_tag="PKN55_09885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HNX71597.1"
                     /translation="MAMEIERRFLVSGDGWRQAPGRVLKQGFIAVSRERVVRVRVDGE
                     QGWLTVKAMVTDASRLEFEYPIPLADAEQMLAQVCPAAMEKIRRRVTVGAHVWEVDEF
                     FGDNAGLVLAEVELQDEAEPFERPAWLGAEVTADGRYTNAHLSQHPYSRWPQGHTTQD
                     AHS"
     gene            2553..3836
                     /gene="hemL"
                     /locus_tag="PKN55_09890"
     CDS             2553..3836
                     /gene="hemL"
                     /locus_tag="PKN55_09890"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019921329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HNX71598.1"
                     /translation="MSRNHTLFERARHSIPGGVNSPVRAFGSVGGTPIFFQRGQGAYL
                     WDADNTQYIDYVGSWGPAIVGHAHPEVIAAVQAAAVNGLSFGAPTEAEILIAEEIIKL
                     VPSIESVRLVSSGTEATMSAIRLARGFTGRDKIVKFEGCYHGHSDSLLVKAGSGLLTF
                     GTPSSGGVPADFTRHTLVLEYNNVEQLTQTFAELGDQLACVILEPFAGNMNLIRPSAE
                     FVAALRELCTRHGAVLIYDEVMTGFRVALGGAQELHGIRPDLTTLGKVVGGGMPIGAF
                     GGRADIMACIAPLGSVYQAGTLSGNPVAVAAGLTTLKLIQAEGFHARLGERTRQLVDG
                     LAAAAREAGVTCSTDSVGGMFGLYFTAEVPRSYAEVMQSDRELFNRFFHAMLAEGVYL
                     APSAFEAGFVSAAHTEADIDATVAAARRAFAKLAA"
     gene            complement(3890..4900)
                     /locus_tag="PKN55_09895"
     CDS             complement(3890..4900)
                     /locus_tag="PKN55_09895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019919936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HNX71599.1"
                     /translation="MEISREYGADQAGVLYGFAFRPDTAGLELPATQWPARLRTGLDD
                     GGFVWLHVNLAHSGSEKWLRAHLGLPEDFYAGLREGPASTRIEQLDGEALRAVINDVI
                     FTFGSPAVEVSSLWVYADAHLLITARLKPLRSVDALRAAVKRGVRFGSPVDLLVHLLS
                     GQADVLAQIVRDASQAVDAIEDRQLAERPRHSRAELASMRRVLVRLQRLLAPEPAALF
                     RLLNRPPAWLDADAVQGLRESSEEFAVALNDLGALTERLKLLQEELAAQLNEQSNRTL
                     FTLTLVTVLALPINIVAGLFGMNVGGIPLAEHPHGFWVLVGLIAAFTVLAGSWAFGRQ
                     RP"
     gene            5195..5479
                     /locus_tag="PKN55_09900"
     CDS             5195..5479
                     /locus_tag="PKN55_09900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011136857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX71600.1"
                     /translation="MQFDEVKPEHFSTISRDPFPHILIDRALLQISGGDARAAKFRVD
                     ALAAAGWAHSGLVPFGRYPDQAAAAFNRLRTVLEITEKPAQVLAELQKQG"
     gene            5560..5635
                     /locus_tag="PKN55_09905"
     tRNA            5560..5635
                     /locus_tag="PKN55_09905"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5593..5595,aa:Thr,seq:cgt)
BASE COUNT          963 a         1875 c         1950 g         1109 t
ORIGIN      
        1 gtgatgccgc caatcgccac gcgtggcgcg cccagcgcgg cgctgtcggc aaacaggctg
       61 agcggggcgc gcacggcatt cggtttggtg gcggaggggc agacggcgcc aaaggccacg
      121 tagtcggcgc cagcggcgat ggcggcgtgc gccagcggca gttggtcgta gcacgaggcg
      181 ccgaggatgg cgtgttcgcc caggcgggcg cgggcggcgg cgatgtcgcc gtcgtcgcgg
      241 cccaggtgca cgccgtcggc gcccacttgg gcggccagcg ccacgtcgtc attgatgatc
      301 agcccaacgc cggcggcgcg ggtgagcgcg cgcagggttt gcgcctgcgc caggcgcagc
      361 gccgggtcgg tggatttatt gcggtattgc agcagcgccg gccgcgccgg cagcacgcgc
      421 aggcactggc tgaacaggcg caggctgtcg gtggtgtccg gggtgatcag atacagccca
      481 cggcggggca gggcgtcagg ccaggccatc gggctctcct gcggcagggg cttcgtcttc
      541 ggcggtggcg tcgccgcgcg cccagaacag gcggtcgggg atggcctggc ccatgccggg
      601 gcggaaggcg cggttgaggg tttcccaggt gtaggcctgc gcctcgcgca cggcgtcggg
      661 catgctcagg ccgttggcca gcgagccggc gatggccgag gccagggtac agcccgagcc
      721 gtggtagctg ccgggcagcc gtggccagtg ttcgcgcgag gtcatgccgc tgcggtgata
      781 cagggtgttg cacacctgca cggtgttgtc gtgggtgccg gtgatcagca cgtattccac
      841 accggcctgc aagagccgct gcgccgcctg cggcagcgag agctcgtctt cttcgtccgg
      901 gttgctggcc gccagccggc gggcttcgat gctgttgggg gtgaccagcg tggtcagcgg
      961 cagcagcaga tcgcggatgg cttcgcacag ctcttcgcgc gccagctcgt cgccgccgcc
     1021 cgaggccagc accgggtcga ggatcagcgg cacgtccggg tagtcggcgg cgatttcagc
     1081 aatcacggcg gcgttttcca cgctgcctat catgcccacc ttgaacaccg acaccggcat
     1141 gtcttccagc agaaagcgcg cctgatcaga cacttgctcg gcgtccatca cccagaagtt
     1201 ttccacgctg cgggtgtctt gcacggtgac ggcggtgatc acggacaagg ggtgacagcc
     1261 caggctggcc agcgtcagga tgtcggcctg gatgcctgcg cccccggaag ggtcggcgcc
     1321 agccagggtc atgacgatgg gcggaaattc ggcgatttgg gacaagatgg attctcgaac
     1381 ggggtgaaca atgtagaatt tcattttacc tgtcccaccc cggagcaacc acatgaaaac
     1441 ctggatgtgc ctgatttgcg gctttatcta tgacgaggcg caaggccgtc ccgaagacgg
     1501 catcgcgccg ggcacccgct gggaagatgt cccgatgaac tggacctgcc cggactgcgg
     1561 cgcgcgcaag gacgattttg aaatggtcga gttctgacca ctgcacaagg gctggctgat
     1621 ggattacctg attatcaagg ccttgcatct gttttttgtc atcgcctggt ttgccgggct
     1681 gttttatctg ccgcgcattt ttgtcaatct ggccatggcc ggcgatatgg tcgagctggc
     1741 gcggctgaag ctgatggccg gcaagctgta ccgctttatg accccgctgg ggctgattgc
     1801 cctggcgctg ggcctggtgc tgtggctggg ctatggcgtc agcggcggct ggctgcacgc
     1861 caagctgacg ctggtggcgg gcttgattgt ctatcacctg atgtgccggc actttttgca
     1921 ggtgtttatc gccgacgcca acacccgcag ccacacctgg tttcgcgtgt tcaatgaaat
     1981 cccggtgttg atgctgctgg cggtggtgct gctggtgatt gtgaaacctt tctgaagatg
     2041 tctggcgcag gagcgccatc cgcaatggca atggaaattg aacgccgctt tttggtcagc
     2101 ggcgacggct ggcggcaggc gccgggccgg gtgctcaagc agggctttat cgccgtgtcg
     2161 cgcgagcgcg tggtgcgcgt gcgcgtggac ggcgagcagg gctggctgac ggtcaaggcc
     2221 atggtcaccg acgcctcgcg gctggagttt gaatacccga ttccgctggc cgacgccgaa
     2281 cagatgctgg cgcaggtgtg cccggcggca atggaaaaaa tccgccgtcg cgtcaccgtg
     2341 ggcgcgcatg tctgggaagt cgatgaattt tttggcgaca acgccggtct ggtgctggcg
     2401 gaagtcgaat tgcaggatga agccgagccg ttcgagcggc cggcctggct gggggcggaa
     2461 gtcaccgccg acggccgcta caccaacgcc cacctgagcc agcatccgta ctcgcgctgg
     2521 ccgcaggggc acactacgca ggatgcacat tcatgagtcg caaccacacc ctttttgaac
     2581 gcgcccgcca ttccatcccc ggcggcgtca actcgccggt gcgcgccttt ggcagcgtgg
     2641 gcggcacgcc gatttttttc cagcgcggcc agggcgcgta tttgtgggat gccgacaaca
     2701 cccaatatat tgactacgtt ggctcctggg gcccggccat tgttggccac gcccaccccg
     2761 aagtgattgc cgccgtgcag gcggcggcgg tgaacggcct gtcgtttggc gcgccgaccg
     2821 aagccgaaat cctgatcgcc gaagaaatca tcaagctggt gccgtcgatt gaatcggtgc
     2881 gcctggtgtc gtccggcacc gaagccacca tgagcgccat ccgcctggcg cgcggcttta
     2941 ccggccgtga caagatcgtc aagtttgaag gctgctacca cggccattcc gacagcttgc
     3001 tggtgaaagc cggctcgggc ctgctgacct ttggcacgcc cagctcgggc ggcgtgccgg
     3061 cggatttcac ccgccacacc ctggtgctgg aatacaacaa cgtcgaacag ctgacgcaaa
     3121 cctttgccga gctgggcgac cagctggctt gtgtgattct ggagccgttt gccggcaata
     3181 tgaatctgat ccgccccagc gccgagtttg tcgccgcgct gcgcgagctg tgtacccgcc
     3241 atggcgcggt gctgatttac gatgaagtga tgaccggctt ccgtgtggcg ctgggcggcg
     3301 cgcaagagct gcacggcatc cgcccggacc tgaccacgct gggcaaggtg gtgggcggcg
     3361 gcatgccgat tggcgccttt ggcggccgcg ccgacatcat ggcctgcatc gccccgctgg
     3421 gttcggtgta tcaggctggc acgctgtccg gcaatccggt ggcggtggct gccgggctga
     3481 ccacgctcaa gctgattcag gctgaaggct tccacgcccg cctgggcgag cgcacccgcc
     3541 aactggtcga tggcctggcc gccgccgccc gcgaagctgg cgtaacgtgc agcaccgaca
     3601 gcgtgggcgg catgttcggg ctgtatttca ccgctgaggt gccgcgcagc tacgccgagg
     3661 tgatgcagtc cgaccgcgag ctgttcaacc gcttcttcca cgccatgctg gccgaaggcg
     3721 tgtatctggc gccgtcggcg tttgaggcgg gctttgtgtc tgctgcgcac accgaagccg
     3781 acatcgacgc caccgtggcg gcggcgcgcc gggcattcgc caaactggcg gcgtgatgcg
     3841 ggtttgcgcc cactgcgcca taaaaaacgc ccgctgacgc gggcgttttt tatggccgtt
     3901 gccggccaaa agcccagctt cccgccagga cggtgaacgc cgcaatcaaa cccaccagca
     3961 cccaaaaacc atgtgggtgc tcggccagcg gtatcccacc gacgttcatg ccaaataagc
     4021 ccgccacgat attgatcggc agcgccagca cggtgaccag ggtcagggtg aacagcgtgc
     4081 ggttgctttg ctcgttgagc tgggcggcca gttcttcttg caagagcttc agccgctcgg
     4141 tcagcgcgcc caggtcgttc agcgccacgg caaattcttc gctggattcg cgcaagccct
     4201 gcaccgcgtc ggcgtccagc caggccggtg ggcggttaag caggcgaaac agcgccgccg
     4261 gctcgggggc cagcagccgt tgcaggcgca ccagcacccg acgcatgctg gccagctcgg
     4321 cgcggctgtg gcgcgggcgt tcggccagtt ggcggtcttc gatggcgtcc accgcctgcg
     4381 aggcgtcgcg cacgatctgc gccagcacat cggcctgacc gctgagcaga tgcaccagca
     4441 aatccaccgg cgagccaaag cgcacgccgc gcttcaccgc cgcgcgcagc gcatccaccg
     4501 agcgcaaggg tttaagccgg gcggtaatca gcaaatgcgc gtcggcgtac acccacagcg
     4561 atgagacttc caccgccggg ctgccaaaag tgaaaatcac atcgttgatt accgcgcgca
     4621 gcgcctcgcc gtccagttgc tcgatgcgag tcgaggctgg gccttcgcgc aggccggcgt
     4681 aaaaatcttc tggcaggccc aggtgcgcgc gcagccattt ttcgctgccg ctgtgcgcca
     4741 gattgacgtg cagccagaca aaacctccat cgtccaggcc ggtgcgcagg cgcgccggcc
     4801 actgggtggc tggcagctcc agcccggcgg tgtcgggccg aaaagcaaaa ccatacagca
     4861 cgcccgcctg atctgcgccg tattcccgcg agatttccat tgccttgctc ctgctttgcg
     4921 ccatccagcg gccagcctgc cagcgggata tgacaaaacg tcgtgcctgc tgtgcgaatt
     4981 cctgacccgc cgttcagtgt gggcgcagca cttgcgcgcc ccgcgcaccc atgccatgct
     5041 tgtctgtcgg caatccgcgc cgtgtctgtc tttcaccgcg ctgcgctctg tgcttttgct
     5101 cggggcgttg cctggtagca ccgtttcaag cgtttgtttc cgtgttctcg cggggacagt
     5161 gaaatcttcc gtggccacag aaggaacccg aatcatgcag tttgatgaag tcaaacccga
     5221 acacttttcc accatctccc gcgacccgtt tccgcacatc ctgatcgacc gcgccctgct
     5281 gcaaatcagc ggcggcgacg cccgcgccgc caagttccgc gtggacgccc tggccgctgc
     5341 cggctgggca cacagcggcc tggtgccgtt tggccgttat ccggatcaag ctgccgccgc
     5401 gttcaatcgc ctgcgcaccg tgctggaaat caccgaaaaa ccggcacaag tgctggccga
     5461 gctgcaaaag caaggctgat gtcggcggtc ggcgggtggt tcacaagccg tgcattcatt
     5521 gctagaatgc gcgcttgtgt ctgaaacacc cagaccggcg ccggtatagc tcagttggta
     5581 gagcagcgca ttcgtaatgc gaaggtcgta ggttcgactc ctattaccgg caccatccac
     5641 tgcaagcctc cccatttttg gtgaggcttg ttgttttttg tgcctatctt gatccggcgc
     5701 aaacattctg cccgtccgcc tcctgttctc tgccggattg tgcatacttt ttgcacagct
     5761 tctgcccatg acagtcaacg cgaggccatc atgtcggatt ccatacacgt cctgcctttc
     5821 cagcgcctgg gccagcagta tcagtgccag gcgcgctacg gtttttttcg caacggcatg
     5881 ccctgctatt tgctgga
//
LOCUS       DAOPYX010000190         5879 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_16854_length_5879_cov_9.547390, whole genome shotgun sequence.
ACCESSION   DAOPYX010000190 DAOPYX010000000
VERSION     DAOPYX010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 5879)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5879)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5879
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16854_length_5879_cov_9.547390"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(40..3144)
                     /locus_tag="PKN55_09910"
     CDS             complement(40..3144)
                     /locus_tag="PKN55_09910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018151088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNX71601.1"
                     /translation="MWLTRVAIGNPYLAAVAMLAIVVLGLFSWQRLSVEEFPDIRFPV
                     AVVTVGYPGAAPSVVESEVTRPLEEAVNAINGVKNIRSYSFEGSSVVVVEFVLTIDPN
                     RAVQDVRDKVAGVQGRFRREIGVPTVNQVNPNDDPMMTVIVASASASPRTLTTHADQV
                     VRKRLQTVPGVGEVQLVGGVKRELRVHIDPYRMQALGLDTTEVVNALRNANQDFPAGR
                     VQDINRDYSVRLSGKLASPEAFGQITVGVRNGAPIRLDDVAQVLDGQEEYNSLALING
                     QRALSIEIKSARGANVVDTSAGVYAALDELRKNLPAGMTLTVIKDQSRQVKNSLADVK
                     MTLLEGAALTVLIVFVFLASWRSTVITGLTLPIALIGTLFAIAVAGFTLNVMTLLALS
                     LSIGLLIDDAIVVRENIVRHARMGKGHYQAAVDGTEEIGLAVLATTLTVVAVFLPVGF
                     MGGIIGKFFQQFGLTVTVAVLISLLVSFTLDPMLSSIWHDPHAAGTAARGRLAQALAR
                     FEAWMDRQAERYARALAWALAHRKTTLAAASALFVGSLLLAPVVGGEFVPKSDTGKFI
                     VKFKTAPGSSLEYTEAKAREVEALLRRQPEIKDVYSNIGGGFAAGRNQATLRVFTTPK
                     AERERSVFALFAPIRQDITQLAGVQLESIEAEGGPGGGGKPVRVGIRGADFATLAVIA
                     NQLRGKLAAIPHVSDVESSFDDADPALSVEVKRDAAARAGVNLDRVGATLSTLLAGTT
                     ATTWEAPDGENYDVRVQIPRDARSTELLDVLTVASDQRDADGQARMIPLSTVADVKVS
                     TTPRQIDRMDQAREVTLTANIQGKDARQVFQAIDQLLKDTPLPPGYRFDHGGQSKDMA
                     ESFSYAVQALAIGVIFIYMILVAQFRSFSQPLAIMMSLPLGIIGVMLGLLLWGSTLNL
                     FSVIGVIMLMGLAAKNGILLVDFANQARREGMSLHDALLEAGRVRMRPILMTSFAMIF
                     GMLPLAMALGEGAETRAPMAHAVIGGMVSSTVLTLIVTPVVYSYLEGVAAWWRGRKAK
                     TRPA"
     gene            complement(3157..4263)
                     /locus_tag="PKN55_09915"
     CDS             complement(3157..4263)
                     /locus_tag="PKN55_09915"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNX71602.1"
                     /translation="MTSSVFSLRAALLALLCMLSALVLAATDAPPAARPALKLSPQDL
                     AVASRAPLAERLSFTGALQALRATTLAARTEGVAVEVSVREGEAVRAGQVLARLEAAD
                     LQERVNEQAALVATERARLALARQKLEKQRELYAQNFISKLAFDELDSNFQVSQAQLR
                     AQEAQLARARKALEDTVIRAPFAGVIHERAINLGQRVAVNGKLFALADLTQLEIAASV
                     PARQIARIAIGQRARFTVEGGAREFAGQVARINPVALAGTRSFNVFIAVDNRDGQLRG
                     GQFAQGALLLRDAGQALSLPIPAIRDAASTQPWVLKLVGDKAVRQAVTLGVRDDAANR
                     VQIVAGLQDGERVLLAGVLGVQAGDRVQAPAVGK"
     gene            4582..5679
                     /locus_tag="PKN55_09920"
     CDS             4582..5679
                     /locus_tag="PKN55_09920"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HNX71603.1"
                     /translation="MVGVPLPWDIYTKGNRLILRKGNMILNDKQRDQLLKLGLYVRSV
                     ATPEDYTAPKPVNEPFNPFRRLDQVYASLDAMLARPASFRHEFGKRIRSLAVVVDETV
                     QKAPDACIGSIFLLPFTNYSSAHSLHVSLLLAILMQRLGLTLPERISLLSAALTMNIG
                     MYEVQNQLYHQALPLSDNQRMEIDHHPEESVRILQDCFVGDELWLRVVLEHHENWEGS
                     GYPYRMTKEEIHPLSHLLYLADVVGAKLTPRRYRDPVRPNVALSQVFMNRGKSIDMQY
                     AALLVKELGIYPPGSYVRIRNGDVALVTHRGANASTPRVMSIASGQGMPYAMPIPRDT
                     RSAEFGIETSLPAADSIKITNLSKIWGYVLA"
BASE COUNT         1061 a         1928 c         1854 g         1036 t
ORIGIN      
        1 cttggcgtca gtccatcgct ttgctgatgc tgcaagcgct catgccgggc gcgtcttggc
       61 ttttcgtccc cgccaccacg ccgccacgcc ttccagatag ctatacacca ccggggtgac
      121 gatcagcgtc agcacggtgg agctcaccat gccgccaatc accgcgtggg ccatgggcgc
      181 gcgggtttcg gcgccttcgc ccagcgccat ggccagcggc agcatgccaa aaatcatggc
      241 aaagctggtc atcagaatcg ggcgcatgcg cacgcggccg gcttccagca gcgcgtcgtg
      301 cagcgacatg ccttcgcggc gcgcctggtt ggcgaaatcc accagcagaa tgccgttttt
      361 cgccgccagg cccatcagca tgatcacgcc gatcaccgag aacaggttca gcgtgctgcc
      421 ccacagcagc agccccagca tcacgccgat gatgcccagc ggcagcgaca tcatgatggc
      481 cagcggctgg ctgaagctgc ggaactgcgc caccagaatc atgtaaataa agatcacgcc
      541 aatcgccagc gcctgcaccg catagctgaa cgattcggcc atgtccttgc tttgcccgcc
      601 gtggtcaaaa cgatagccgg gcggcagcgg ggtgtctttc agcagttggt cgatggcctg
      661 gaacacctgg cgggcgtcct tgccctggat attggcggtg agggtgactt cgcgcgcctg
      721 gtccatgcgg tcgatctggc gcggggtggt ggacactttc acgtcggcca cggtggacag
      781 cggaatcatg cgcgcctggc cgtcggcgtc gcgctggtcg ctggccacgg tgagcacgtc
      841 caaaagctca gtcgagcggg cgtcgcgcgg aatttgcacc cgcacatcgt agttttcgcc
      901 atccggggct tcccaggtgg tggcggtggt cccggccagc agggtggaca gcgtggcgcc
      961 cacgcggtcc agattcacgc ccgcgcgggc ggcggcgtcg cgcttgacct ccaccgacag
     1021 cgccgggtcg gcgtcgtcaa agctggattc cacatcgctg acatgcggga tggcggccag
     1081 cttgccgcgc agctggttgg cgatgacggc cagggtggca aagtcggcgc cgcgaatgcc
     1141 cacgcgcacc ggcttgccgc cgccgcccgg cccgccctcg gcttcaatcg attccagctg
     1201 cacgccggcc agttgggtga tgtcctggcg gatgggggca aacagggcaa acacgctgcg
     1261 ttcacgctcg gccttggggg tggtgaacac gcgcagggtg gcctgattgc ggccggcggc
     1321 aaacccgccg ccgatattgc tgtacacgtc tttgatttct ggctggcggc gcagcagggc
     1381 ttccacctcg cgcgccttgg cttcggtgta ttccagcgat gaaccgggcg cggtcttgaa
     1441 tttgacgatg aatttgccgg tgtcgctctt gggcacaaac tcgccgccca ccacgggcgc
     1501 cagcagcagg ctgccgacaa acagcgcgct ggcggcggcc agcgtggttt tgcggtgggc
     1561 cagcgcccag gccagcgcgc gggcgtagcg ttcggcctgg cggtccatcc acgcttcaaa
     1621 gcgcgccagc gcctgcgcca ggcggccgcg cgcggcggtg ccggcggcgt gcgggtcgtg
     1681 ccagatcgac gacagcatcg ggtcgagggt aaagctcacc agcagtgaga tcagcaccgc
     1741 cacggtgacg gtcaggccaa attgctggaa aaacttgcca ataatcccgc ccataaagcc
     1801 caccggcagg aacaccgcca ccacggtcag cgtggtggcc agcaccgcca ggccgatttc
     1861 ttcggtgccg tccaccgccg cctggtaatg tcccttgccc atgcgcgcat ggcggacgat
     1921 gttctcgcgc accacgatgg cgtcgtcgat caacaggcca atcgacaagg acagcgccag
     1981 cagggtcatc acattcaggg tgaagcccgc cacggcgatg gcgaacagcg tgccaatcag
     2041 cgcaatcggc aaggtcagcc cggtgatcac cgtgctgcgc cacgaggcca gaaacacaaa
     2101 cacgatcagc acggtcagcg ccgcgccttc cagcagggtc atcttgacgt cggccagcga
     2161 gtttttcacc tgacgggatt gatctttaat gacggtcagc gtcatgccgg cgggcagatt
     2221 tttgcgcaac tcgtccagcg cggcgtacac cccggccgag gtgtccacca cattggcgcc
     2281 gcgcgcgctc ttgatttcga tggacagcgc gcgctggccg ttgatcagcg ccagcgagtt
     2341 gtattcttcc tggccgtcca gcacctgggc gacgtcgtcc agccggattg gcgcgccatt
     2401 gcgcacgccg acggtgatct ggccaaacgc ctcgggcgag gccagtttgc cggacaggcg
     2461 caccgaataa tcgcggttga tgtcctgcac ccgaccggcc ggaaaatcct gattggcgtt
     2521 gcgcagggcg ttgaccactt cggtggtgtc cagccccagc gcctgcatgc ggtaggggtc
     2581 gatatgcacg cgcagctcgc gcttgacgcc gcccaccagc tgcacttcgc ccacgccggg
     2641 cacggtttgc aggcgcttgc gcaccacctg gtcggcgtgg gtggtcagcg tgcgcgggct
     2701 ggcgctggcc gaggcgacaa tcaccgtcat catcgggtcg tcgttggggt tgacctgatt
     2761 gacggtaggc acgccgattt cgcggcgaaa gcgcccctgc acgccggcca ctttgtcgcg
     2821 cacatcctgc acggcgcggt tggggtcgat ggtgagcaca aactcgacca ccaccactga
     2881 cgagccttca aacgaatacg agcggatgtt tttcacgcca ttgatggcgt tgaccgcttc
     2941 ttccagcggc cgggtgactt cgctttccac cacgctgggc gcagcgcccg gatagcccac
     3001 ggtgaccacc gccaccggaa aacgaatatc gggaaactcc tccaccgaga gccgctgcca
     3061 ggaaaacaaa cccagcacga caatggccag catggccacc gccgccagat aggggttgcc
     3121 aatcgccaca cgggtcagcc acatcgcgcc gcccccttat ttgccgacgg ccggcgcttg
     3181 cacgcggtcg cccgcctgca cgcccagcac cccggccagc aacacgcgct cgccgtcttg
     3241 caggccggcg acaatctgca cgcggttggc ggcgtcgtcg cgcacgccca gcgtcaccgc
     3301 ctggcgcacg gccttgtcgc ccaccagctt gagcacccag ggctgggtgc tggcggcgtc
     3361 gcggatggcg ggaatcggca gcgacaacgc ctggccggcg tcgcgcagca gcagcgcgcc
     3421 ctgggcgaac tggccgccgc gcagctggcc gtcgcggttg tccacggcga tgaacacatt
     3481 gaaactgcgc gtgccggcca gcgccaccgg gttgatgcgc gccacctggc cggcaaactc
     3541 acgcgcgccg ccctctacgg taaagcgggc gcgctggccg atggcgatgc gggcaatttg
     3601 ccgcgccggc acgctggcgg cgatttccag ctgggtgagg tcggccaggg caaacaattt
     3661 gccgttcacc gccacccgct ggcccagatt gatggcgcgt tcgtgaatca ccccggcaaa
     3721 cggcgcgcgg atcaccgtgt cttccagcgc cttgcgcgcc cgcgccagtt gggcttcttg
     3781 tgcgcgcagc tgggcctggc tgacctgaaa attgctgtcc agctcgtcaa acgccagttt
     3841 ggagataaag ttctgcgcgt acagctcgcg ttgtttttcc agcttttgcc gggccagcgc
     3901 cagccgcgcg cgttcggtgg ccaccagcgc ggcctgctcg ttcacccgct cttgcaggtc
     3961 ggcggcctcc agccgcgcca gcacctggcc ggcgcgcacc gcctcgccct cgcgcacgct
     4021 gacttccacc gccacgcctt cggtgcgcgc cgccagcgtg gtggcgcgca gggcttgcaa
     4081 ggcgccagtg aacgacaggc gttcggccag cggcgcgcgg ctggcgacgg ccaggtcctg
     4141 cggagagagt ttcaatgccg gacgagccgc aggcggagca tcagtggcag ccagcaccag
     4201 ggcgctcagc atgcacagca gcgccagcag cgcagcgcgg agtgaaaaaa cagaagaagt
     4261 catggtgtga gtgttctggg caaaggagcc gtgggtagac gttgcaaaac ggccaaaagt
     4321 ttgcggcgtt tacataagac ttggcaagtg ctgaagattt ccattgattg cataaaaaat
     4381 acagcaattc atgccatatg attattttac cgacaattac ccggtaatcc gggcaaaaaa
     4441 ccggtgggtg gcttgtttga cctgccccgc ctccctagaa tactgcggca ttgccgccca
     4501 cggccagggc tctcccctga gccatcggcg agttcaacgg ggaacctgca tattatggaa
     4561 gagttggaac caagacacct gatggtgggc gttccgctgc cttgggacat ctacaccaag
     4621 ggcaaccggc tgattttgcg caaaggcaat atgattctca acgacaagca gcgcgatcaa
     4681 ctgctgaagt tggggctgta tgtgcgcagc gtggccacgc ccgaggatta caccgcgccc
     4741 aaacctgtca acgagccatt caaccccttc cgccggctgg atcaggtata cgcttcgctg
     4801 gacgcgatgc tggcgcgccc ggccagtttc cgtcatgaat tcggcaagcg catccgctcg
     4861 ctggcggtgg tggtggacga aaccgtgcaa aaagcaccag acgcctgcat tggctcgatt
     4921 tttttgctgc cgttcaccaa ttattcttct gcgcactcgc tgcacgtcag cctcttgctg
     4981 gccatcctga tgcagcgcct tggcctcacc ctgcccgagc gcatcagcct gctgtcggcg
     5041 gcgctgacca tgaatatcgg catgtacgag gtgcaaaatc agctgtatca ccaggcgctg
     5101 ccgctgtcgg acaatcagcg catggaaatc gaccatcacc cggaagaatc cgtgcgcatc
     5161 ctgcaggatt gttttgtcgg cgatgaactc tggctgcgcg tggtgctcga acaccacgaa
     5221 aactgggaag gttcgggcta tccctaccgg atgaccaaag aggaaatcca tccgctgtcg
     5281 catctgctgt atctggccga tgtggtgggc gccaagctga cgccgcgccg ctaccgcgac
     5341 ccggtgcgcc ccaatgtggc cttgtcgcag gtgttcatga accgtggcaa atccatcgac
     5401 atgcaatatg ccgcactgct ggtgaaagaa ctgggcattt acccgccagg ctcgtatgtg
     5461 cgcatccgca atggcgatgt ggccttggtc acccaccgcg gcgccaatgc cagcaccccc
     5521 agggtgatga gcatcgccag cggccagggc atgccctatg ccatgccgat tccgcgcgac
     5581 acccgttcgg ctgaattcgg catcgaaact tcattgccag cggcggattc gatcaagatc
     5641 accaatctgt ccaagatttg gggttacgtg ctggcctgac aatttgtctg tgtgcttggc
     5701 cctgccgttg cctacctcgg cgtggcgggt taactgtgcg acgccagatg gcgtccatgc
     5761 ggccaggcgg catggacaaa accagcacgg tgatctggcc aggtgggcgt tcggagctaa
     5821 gagtcgctca caaaactcag cgaagcgccc ctggctaggc gtgtgaccgc agacagtac
//
LOCUS       DAOPYX010000191         5865 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_16912_length_5865_cov_8.617900, whole genome shotgun sequence.
ACCESSION   DAOPYX010000191 DAOPYX010000000
VERSION     DAOPYX010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 5865)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5865)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5865
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16912_length_5865_cov_8.617900"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(5..985)
                     /gene="astE"
                     /locus_tag="PKN55_09925"
     CDS             complement(5..985)
                     /gene="astE"
                     /locus_tag="PKN55_09925"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: protein motif:HMM:NF003706.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009017 - succinylglutamate desuccinylase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HNX71604.1"
                     /translation="MMGDLLAAVLHDRPALTAARPLAGGGQVEEWDQGVWCFTPAAPS
                     QFDLVLSAGVHGNETAPVELLDQLGQALLDGSQPLRCRLLLILGNPAALRQGVRYLDE
                     DMNRQFGAQPGGGRDAQRAHALRQHIQRFFADGAPTALRVHYDLHTAIRGSLIEQFAL
                     CAAPHPAEARFDPLAWLAAAGMRAALLYQRSAPTLTAWTHSQHHAHAATLELGKARPF
                     GHNQAIDLSQLAQQLRHWLASPTLPAAPNTAPPPMPCFRVARDIPRLSDAFRLHLAAD
                     TANFTPLHPGQLLAEDGDTRYVIDEQDARVIFPNPNVANGQRAGLVIVPW"
     gene            complement(988..2334)
                     /gene="astB"
                     /locus_tag="PKN55_09930"
     CDS             complement(988..2334)
                     /gene="astB"
                     /locus_tag="PKN55_09930"
                     /EC_number="3.5.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018748291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009015 - N-succinylarginine dihydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
                     /protein_id="HNX71605.1"
                     /translation="MHAFEANFDGLVGPTHNYAGLSYGNVASTGNGQRAANPRLAARQ
                     GLDKMKALADLGFRQGVLAPHERPDVASLRQLGFAGSDAQVIARAAREAPAVLAACSS
                     ASPMWTANAATVSPSADSADGRVHFTPANLNNKFHRAIEHPTTGRVLAATFADPRCFA
                     HHPALPAHMAFGDEGAANHTRLCADYGAPGVQLFVYGQRAFDASANGPRRYPARQTLE
                     ASQAVARQHGLNPARVVFAQQAPAAIDAGVFHNDVIAVGNRDVLFYHQHAFADADATL
                     AALADAMHGVGASLRPLQVPAAAVSIEDAVRSYLFNSQLLSRADGRMLLVVPEECRHV
                     EPVWAYLQDLLASQGPIAEVRVFDLKQSMQNGGGPACLRLRVALTADECAAVNPAVWI
                     NDARYASLCAWVDRHYRDRLSADDLADPQLLQECRTALDELTQLLQLGSIYPFQRG"
     gene            complement(2346..3812)
                     /gene="astD"
                     /locus_tag="PKN55_09935"
     CDS             complement(2346..3812)
                     /gene="astD"
                     /locus_tag="PKN55_09935"
                     /EC_number="1.2.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008954224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
                     /protein_id="HNX71606.1"
                     /translation="MTQYFAGQWQAGAGAPFASLDPVDQRPLWQGRAADAAQVDAAVA
                     AARAAFADWRDAGLDARAAVARRFAQLLGEQQAELAALIGQETGKPRWEALTEVQSMI
                     AKVDISLKAQAERAGERAQTMGDAQAVLRHRPHGLVAVFGPYNFPGHLPNGHIVPALL
                     AGNCVLFKPSELTPAVAEASVRLWLAAGLPAGVLSLLQGGRDTGAALAGHDGVDGLFF
                     TGSSSTGQQLHQLYAGRPDKILALEMGGNNPLIVDHVADVDAALHHIVQSAFVSAGQR
                     CTCARRLLVARGAEGDALLARLTAVCAALRVGRWDDEPAPFMGAVISPAAARRLLDSQ
                     AALLAAGGVSLLAMRELAPGSALLSPGLIDVSAVTALPDEEDFGPLLKVQRYDDLEHA
                     FTLANATRYGLAAGLLSDRREVYERFWRAMRAGVVNWNKPLTGASSAAPFGGVGASGN
                     HRASAYYAADYCAYPVAGLEAATAQLPASLPPGLTLNP"
     gene            complement(3823..4860)
                     /gene="astA"
                     /locus_tag="PKN55_09940"
     CDS             complement(3823..4860)
                     /gene="astA"
                     /locus_tag="PKN55_09940"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018748289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008791 - arginine N-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HNX71607.1"
                     /translation="MRVRSIESADLPALLALAQSTGVGVTTLPANAERLGRRIDASLA
                     TLAQAPDPARASLLFVLDDPARPQHVVGICGLEGALGMSEPWYNYRVGAAVHASRELG
                     LYRRLETLFLSNDLTGAAELCSLFLHPDWRRDGNGSLLSKSRFLFLAEFPEYFSPRVI
                     AEMRGVSDEHGRSPFWESLGRHFFSMEFSEADYLTGIGNKSFIAELMPQHLIYTAFLS
                     APARAVIGQVHANTRPALALLTGEGFRYNGYVDIFDAGPAVECPLADIRAVRDSLCLL
                     AADRPPPAQAQPWLVSNRRRHGFAVTLAHTAPDADGQLPLDAQALAALAIAPGDSVRA
                     VPLSRPHPNRP"
     gene            complement(4888..>5865)
                     /locus_tag="PKN55_09945"
     CDS             complement(4888..>5865)
                     /locus_tag="PKN55_09945"
                     /inference="COORDINATES: protein motif:HMM:TIGR03243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HNX71608.1"
                     /translation="PAVEALAAQSPVGVTSLPANREALLHKIQASQDALAAEVRLYGE
                     ERYFFTVEDRASGRLLGLSGLVASAGYNEPFYSFRNELLIHASPELGIHNKIHALSLC
                     HDLTGHSLLTSFFILPELGAGPAAELLSRSRLLFMAGHPARFADTVVSEMLGVTREDG
                     SSPFWDAVGRVFFGLDYHEAELICGVKGRKFIAELMRQHPIYVPLLSAEAQAVIGEVG
                     EGSRIAFDILEREGFETENYLDIFDGGPTLLGRLPGLRSYAGSRVCRVRLGPVQGGGP
                     YLLANGGLSDFCATLARLHPPAHGEVTVPPDVAAALGVAEGEMVRLAAL"
BASE COUNT          930 a         1934 c         2162 g          839 t
ORIGIN      
        1 cgcttcacca cggcacaatc accaaccccg cccgctggcc attggccacg ttcggattcg
       61 gaaaaatcac ccgtgcgtct tgttcgtcaa tcacatagcg ggtgtcgccg tcttcggcca
      121 gcagttggcc tgggtgaagc ggggtgaagt tggcggtgtc ggcggccagg tgcaggcgga
      181 aggcgtcgct caggcggggg atgtcgcggg cgacgcgaaa gcagggcatg ggcggcgggg
      241 cggtgttggg ggcggcgggc agggtggggc tggccagcca gtggcgcagt tgttgcgcca
      301 gttggctcag gtcgatggcc tgattgtggc cgaagggccg ggctttgccc agttccaggg
      361 tggcggcgtg ggcgtggtgc tggctgtggg tccaggcggt gagggtgggg gcgctgcgct
      421 ggtagagcag ggcggcgcgc atgccggcgg cggccagcca ggccagcggg tcaaagcgcg
      481 cctcggccgg gtggggggcg gcgcacaggg caaattgctc gatcagcgag ccacggatgg
      541 cggtgtgcag gtcgtaatgc acgcgcaggg cggtgggcgc gccgtcggca aaaaagcgct
      601 ggatgtgctg gcgcagggcg tgggcgcgct gggcgtcgcg gccgccgccg ggctgggcgc
      661 cgaactggcg gttcatgtct tcgtccagat agcgcacgcc ctggcgcagc gccgccgggt
      721 tgcccaggat cagcagcagc cggcagcgca gcggctggct gccgtccagc agggcctggc
      781 ccagttggtc gagcagctcg accggggcgg tttcgttgcc gtgcaccccg gcggacagca
      841 caaggtcaaa ctggctgggc gcggcggggg tgaagcacca cacgccctgg tcccattctt
      901 ccacttggcc gccgccggcc agcgggcggg cggcggtcag cgccgggcgg tcgtgcagca
      961 cggcggcgag caaatctccc atcatggtca gcctcgctga aaaggataaa tcgagcccag
     1021 ttgcagcagc tgggtgagct catccagcgc ggtgcggcat tcttgcagca gctgcgggtc
     1081 ggccaggtcg tcggcgctga ggcggtcgcg gtagtggcgg tccacccagg cgcacaggct
     1141 ggcgtagcgg gcgtcgttga tccacaccgc cgggttgacc gccgcgcatt cgtcggcggt
     1201 cagcgccacg cgcaggcgca ggcaggctgg gccgccgccg ttttgcatgg attgcttgag
     1261 gtcaaacacc cgcacttcgg caatcgggcc ttggctggcc aataggtctt gcagataggc
     1321 ccacaccggc tcgacgtggc ggcattcttc cggcacgacc aagagcatgc ggccgtcggc
     1381 gcggctgagc agctggctgt tgaacagata actgcgcacg gcgtcttcga tgctgaccgc
     1441 cgccgccggc acttgcagcg ggcgcaggct ggcgcccacg ccgtgcatgg cgtcggccag
     1501 cgcggccagg gtggcgtcgg cgtcggcaaa ggcgtgctgg tgataaaaca gcacatcgcg
     1561 gttgcccacg gcgatcacgt cattgtgaaa caccccggcg tcgatggcgg cgggcgcttg
     1621 ctgggcaaac accacgcgcg ccgggttcag gccgtgctgg cgggccaccg cctggctggc
     1681 ttccagcgtt tgccgggccg ggtagcggcg cgggccgttg gcgctggcgt caaaggcgcg
     1741 ctggccgtag acaaacagct gcacgccggg cgcgccgtag tcggcgcaca gccgggtgtg
     1801 gttggccgcg ccttcgtcgc caaaggccat gtgcgccggc agcgccgggt ggtgggcaaa
     1861 gcagcgcggg tcggcaaagg tggcggccag cacgcggccg gtggtggggt gctcgatggc
     1921 gcgatgaaat ttattgttga ggttggccgg ggtgaagtgc acgcggccgt cggcgctgtc
     1981 ggccgagggg ctgacggtgg cggcgttggc cgtccacatc ggcgaggccg agctgcatgc
     2041 ggccagcacg gcgggcgctt cgcgcgcggc gcgggcaatc acctgggcgt cgctgccggc
     2101 aaagcccagc tggcgcaggc tggccacgtc cgggcgctcg tgcggggcga gcacgccctg
     2161 gcgaaacccc aggtcggcca gggctttcat tttgtccagg ccctggcggg cggccaggcg
     2221 cgggttggcg gcgcgctggc cattgccggt tgaggccaca ttgccatacg acaaaccggc
     2281 gtagttgtgg gtggggccca ccaggccgtc aaaattggct tcgaatgcgt gcatctggcg
     2341 ctccatcagg ggttcagggt caggccgggc ggcaggctgg ccggcagttg ggcggtggcg
     2401 gcttccagcc cggccaccgg gtaggcgcaa tagtcggcgg cgtagtaggc gctggcgcgg
     2461 tggttgcccg aggcgcccac gccgccaaat ggcgcggcgc tggaggcgcc ggtcagcggt
     2521 ttgttccagt tgaccacgcc agcgcgcatg gcgcgccaaa agcgctcgta cacctcgcgc
     2581 cggtctgaga gcagcccggc ggccaggccg tagcgggtgg cgttggccag cgtgaaggcg
     2641 tgttccaggt cgtcatagcg ctgcaccttg agcagcgggc cgaagtcttc ttcgtcgggc
     2701 aaggcagtga cggcgctgac gtcgatcagg ccggggctga gcagggcgct gccgggggcc
     2761 agctcgcgca tggccagcag gctgacgccg ccagcggcca gcagcgcggc ctggctgtcc
     2821 agcaagcggc gggcggcggc gggcgagatc accgcgccca taaacggcgc cggttcgtcg
     2881 tcccagcggc ccacgcgcag cgccgcgcac actgcggtta accgggccag caaggcgtcg
     2941 ccctcggcgc cgcgcgccac cagcagacgg cgcgcgcagg tgcagcgctg gccggcggac
     3001 acaaacgccg attgcacaat atggtgcagc gcggcgtcca catcggccac gtggtcaaca
     3061 atcagcgggt tattgccgcc catttccagc gccaggattt tgtccgggcg gccggcgtac
     3121 agctggtgca gctgctggcc ggtggacgag ctgccggtaa aaaacagccc atccacgccg
     3181 tcatggccgg ccagcgccgc cccggtgtcg cgcccgcctt gcaacaggct gagcacaccc
     3241 gccggcaggc cggcggccag ccacagccgc acgctggcct cggccaccgc cggggtgagc
     3301 tcggagggct tgaacagcac gcagttgccg gccagcagcg ccggcacgat atggccattg
     3361 ggcagatgcc ccggaaagtt atacgggcca aacaccgcca ccaggccgtg cgggcggtgg
     3421 cgcagcaccg cctgggcgtc gcccatggtt tgggcgcgct cgccagcgcg ctcggcctgg
     3481 gctttcagcg agatgtccac cttggcgatc atcgattgca cttcggtcag cgcttcccag
     3541 cgcggcttgc cggtttcctg gccgatcagc gcggccagct cggcctgctg ttcacccagc
     3601 aattgggcaa agcggcgcgc cacggcggcg cgggcgtcca gcccggcgtc gcgccaatcg
     3661 gcaaaggcgg cgcgggcggc ggccacggcg gcgtccacct gggcggcgtc ggcggcgcgg
     3721 ccttgccaca gcggacgctg gtccaccggg tcgagcgagg caaacggcgc gccagcgccg
     3781 gcctgccatt ggcctgcaaa gtattgggtc atcagcacat cctcagggtc gattggggtg
     3841 ggggcggctc agcggcacgg cgcgcacgct gtcgccgggg gcgatggcca gcgcggccag
     3901 cgcctgggcg tccagtggca gttggccgtc ggcgtcgggc gcggtgtgcg ccagggtgac
     3961 ggcaaagccg tggcggcggc ggttggacac cagccagggc tgggcctggg ccggcggcgg
     4021 gcggtcggcg gccagcaggc acaggctgtc gcgcaccgcg cggatgtcgg ccagcgggca
     4081 ttccaccgcc gggccggcgt caaaaatgtc cacatagccg ttgtagcgaa agccttcgcc
     4141 agtcagcagc gccagcgccg ggcgggtgtt ggcgtgtacc tggccaatca ccgcgcgcgc
     4201 gggtgctgac aaaaacgcgg tgtaaatcaa atgctgcggc atcagctcgg cgataaacga
     4261 tttattgcca atgccggtga ggtaatccgc ctcggaaaac tccatgctga aaaaatgccg
     4321 gcccaggctt tcccagaagg gcgagcggcc atgctcgtcg gacaccccgc gcatttctgc
     4381 gatcacgcgc gggctgaaat actcgggaaa ctcggccaaa aacaaaaacc gcgatttcga
     4441 cagcaagctg ccattgccgt cgcgccgcca gtccgggtgc aaaaacagcg agcacagctc
     4501 ggcggcgcca gtgaggtcgt tgctgagaaa cagggtttcc agccggcgat acagccccag
     4561 ctcgcgcgag gcgtgcaccg ccgcgcccac ccggtagttg taccagggct cgctcatgcc
     4621 cagcgcgcct tccagcccgc aaatgcccac cacatgctgg gggcgggccg ggtcgtccag
     4681 cacaaacagc aggctggcgc gcgccggatc gggcgcttgg gccagggtgg ccagcgaggc
     4741 gtcgatgcgc cggcccagcc gctcggcgtt ggccggcagc gtggtcaccc ccaccccggt
     4801 ggattgcgcc agggcaagca gggccggcag gtcggcggat tcaatgctgc gtacgcgcat
     4861 gaacagtctc cagaaacaaa tacagcatca caaagcggcc agacgcacca tctcgccctc
     4921 cgccaccccc agcgcggcgg cgacgtctgg cggcacggtg acttcgccat gcgcgggcgg
     4981 gtgcaagcgc gccagggtgg cgcaaaaatc gctcaggccg ccattggcca gcagatacgg
     5041 cccgccgcct tgcaccggcc ccagccgcac ccgacacacc cggctgccgg cgtagctgcg
     5101 caggccgggc agccggccca gcagggtggg gccgccgtcg aagatgtcca ggtagttttc
     5161 ggtttcaaag ccttcccgct ccaggatgtc aaaggcaatg cgcgagccct cgcccacttc
     5221 gccaatcacc gcctgcgcct cggcgctgag cagcggcaca taaatcgggt gctgacgcat
     5281 cagctcggcg atgaacttgc gccccttcac cccgcaaatc agctcggcct cgtggtaatc
     5341 caggccaaaa aacacccggc ccaccgcgtc ccagaacggc gagctgccat cctcgcgggt
     5401 gacgcccagc atctcggaca ccacggtgtc ggcaaagcgc gccgggtggc cggccatgaa
     5461 cagcaggcgg ctgcgcgaca gcagctcggc cgccggcccg gcgcccagtt cgggcaggat
     5521 aaaaaacgag gtcagcagcg aatggccggt gaggtcgtgg cacagcgaca gggcgtgaat
     5581 cttgttgtgg atgcccaact ccggcgaggc gtggatcaac agctcgttgc gaaagctgta
     5641 aaacggctcg ttatacccag ccgaagccac caagccagac aaacccagca gccggccgct
     5701 ggcgcggtct tccacggtga agaaatagcg ctcttcgcca tacagccgca cctcggcggc
     5761 cagcgcgtcc tgcgaggctt gaattttatg cagcagcgct tcgcggttgg ccggcagcga
     5821 ggtgaccccc accgggcttt gcgccgccag cgcttccacg gccgg
//
LOCUS       DAOPYX010000192         5863 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_16924_length_5863_cov_8.462810, whole genome shotgun sequence.
ACCESSION   DAOPYX010000192 DAOPYX010000000
VERSION     DAOPYX010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 5863)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5863)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5863
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16924_length_5863_cov_8.462810"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..755
                     /locus_tag="PKN55_09950"
     CDS             <1..755
                     /locus_tag="PKN55_09950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012697741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HNX71609.1"
                     /translation="LLYWSGDERGSDFAGPREDFPIAVPIASQCGHAVGVGFAFQYRR
                     QPRVAVVTLGDGATSKGDFYEAMNTAGAWRLPVVFVVTNNQWAISLPARKQTAAATFA
                     QKAIAAGIPGEQCDGNDILAVRDCIGRALERARVGDGPSLIECVTYRLSDHTTADDAR
                     RYRAQDEVSAAWALEPLARLRHFLVDQKLWSKQDEETLLADNQAAVNAAAADYLATPP
                     QALSTLFDYLYAELPADLAAQRQQLLTERGQA"
     gene            752..1729
                     /locus_tag="PKN55_09955"
     CDS             752..1729
                     /locus_tag="PKN55_09955"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012697740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HNX71610.1"
                     /translation="MNTLTLLDAINAALAWELAHDDNVVLFGEDIGKNGGVFRATLGL
                     QERFGEQRVLDAPLAETQIAGMAIGMAAQGLRPVCEIQFAGFMYSTFDHLINHAARLR
                     HRTRGRLSCPMVLRTPVGGGIHAPEHHGESPEAWLAHTPGLKVVSPSSPARAYGLLLA
                     AIRDPDPVIFLEPTRLYRLLKEPVADNGEALPLGAAFTLREGCDVTLVSWGASVLETL
                     QAAEQLAEQGVSAEVIDVASLKPLDMPTILASVAKTGRCVIVHEAARQCGVGAEIAAN
                     LASEGLMSLLAPVERVTGFDTPLPLARLEDEYLPSVARILAAVERVRAY"
     gene            1939..3048
                     /locus_tag="PKN55_09960"
     CDS             1939..3048
                     /locus_tag="PKN55_09960"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012697739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HNX71611.1"
                     /translation="MKTFKLPDLGEGLQEAEIVAWHVNAGDHIVVDQPLLSVETDKAI
                     VDVPSPFAGKVLTLHAKVGDVVALDGALVDIDDGGQVEDSGSVVGDAPTATTAHEVRE
                     HASAAAPAARGVRAAPAVRALASKLGVDLSIVTPSGPDGVILASDVERVAKVLANVAA
                     LTPLRGPRRAMAQNMALAHAEVVPVTLVDDADIHAWAAGEDTTWRLLRAMVAGCRAAP
                     ALNAWFDSQALGVRQLSQIDVGIATDTEEALFVPVLRNVAERSREDLRAALEQMKQDV
                     RARTIPPESLRGHSITLSNFGMFAGRYASPVVMPPTVAIVGAGRARREPVAVGDAVVV
                     HRVLPMSLTFDHRAATGAEAARFLAAMMADLQRPD"
     gene            complement(3143..4009)
                     /gene="accD"
                     /locus_tag="PKN55_09965"
     CDS             complement(3143..4009)
                     /gene="accD"
                     /locus_tag="PKN55_09965"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008953718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HNX71612.1"
                     /translation="MSWLNKLLPPKIKKDNKTGKSAVPEGLWSKCSACEAVLYHTDLE
                     NNLHVCPKCSHHNAVGARARLDMLLDAEGRFEIGSEVTPVDILKFKDSKKYPERLTAA
                     QSSTGEDDALVVMQGSIHNLPVVAAAFEFRFIGGSMGSVVGERFVRGVRAAVEQRAPF
                     ICVSASGGARMQEGLSSLMQMAKTSAALQLLTDHKLPFISVLTDPTMGGVSASFAFLG
                     DVVMAEPGALIGFAGPRVIEQTVRETLPEGFQRAEFLLEKGAIDMIVDRRELRRRMAE
                     VLTLLTRQPAVA"
     gene            complement(4089..4880)
                     /gene="trpA"
                     /locus_tag="PKN55_09970"
     CDS             complement(4089..4880)
                     /gene="trpA"
                     /locus_tag="PKN55_09970"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012696729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HNX71613.1"
                     /translation="MNRIDRCFAELAGRKALIPFITAGDPEPGQTVAMMHALVAGGAN
                     VIELGVPFSDPMADGPVIQRASERALAHGVGLRDVLAMVAQFRADDDATPVVLMGYLN
                     PVEAMGYAEFAKAARAAGVDGLLTVDCPPEEAEALSLALHAEQLAPIYLIAPTTPAER
                     LSGIARHARGYVYYVSLKGVTGAANLDVTEVARRVAEIRQHIQLPIGVGFGIRDGQTA
                     RQVADVSDAVVIGSRLVQAVEAAAPGEAAATLQTLLRDIRAALDA"
     gene            complement(4932..>5863)
                     /gene="trpB"
                     /locus_tag="PKN55_09975"
     CDS             complement(4932..>5863)
                     /gene="trpB"
                     /locus_tag="PKN55_09975"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008953716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HNX71614.1"
                     /translation="HKINNAIGQALLAKRMGKKRVIAETGAGQHGVATATVAARYGME
                     CVVYMGAEDVKRQAPNVFRMKLLGATVVPVESGSKTLKDALNEAMRDWVTNVDSTFYI
                     LGTAAGPHPYPMLVRDFVSVIGEESKVQMPEMIGRQPDAVVACVGGGSNAIGIFYPYI
                     PVEGVRLIGVEAGGDGLDSGRHAAPLSAGRPGVLHGARSYLMQDDNGQIIETHSVSAG
                     LDYPGVGPEHSYLKDIGRAEYVAVTDAEALTAFHDLCRYEGIIPALESSHALAYAAKL
                     APTLGKDQVILVNLSGRGDKDINTVAGLAGITL"
BASE COUNT          955 a         2031 c         1933 g          944 t
ORIGIN      
        1 tgctgctgta ctggagcggc gacgagcgcg gcagcgactt tgccggcccg cgcgaggatt
       61 tcccgattgc cgtgccgatt gccagccagt gcggccacgc cgtgggcgtg ggctttgcct
      121 ttcagtatcg ccgccagccg cgtgtggcgg tggtgacgct gggcgatggc gccacctcta
      181 aaggcgattt ttacgaagcc atgaacaccg ccggcgcctg gcgcttgccg gtggtgtttg
      241 tggtcaccaa taaccagtgg gcgatttcgc tgccggcgcg caaacaaacc gctgccgcca
      301 cctttgcgca aaaagccatc gccgccggca ttcccggcga gcaatgcgac ggcaacgaca
      361 tcctggcggt gcgcgactgc atcggccgcg cgcttgagcg cgcccgcgtc ggcgacggcc
      421 cctcgctgat cgaatgcgtg acctaccggc tgagcgacca caccaccgcc gacgacgccc
      481 gccgctaccg cgcgcaggac gaagtcagcg ccgcctgggc gctggagccg ctggcgcggc
      541 tgcggcattt tctggtggat caaaaactgt ggagcaagca ggacgaagaa accctgctgg
      601 ccgacaatca agccgcggtc aacgccgccg ccgccgacta cctggccacc ccgccgcaag
      661 cgctgtccac cctgtttgac tatctgtacg ccgaactgcc ggccgacctg gccgcgcaac
      721 gccagcaact gctgaccgaa agggggcaag catgaacacc ctcaccctgc tcgacgccat
      781 caacgccgcc ctggcctggg aactggcgca cgacgacaat gtggtgctgt ttggcgaaga
      841 catcggcaaa aacggcggcg tgttccgcgc caccctcggc ctgcaagagc gctttggcga
      901 acagcgcgtg ctggatgcgc cgctggcgga aacccaaatc gccggcatgg ccatcggcat
      961 ggccgcgcaa ggcctgcgcc cggtgtgtga aatccagttt gccggcttta tgtattccac
     1021 ctttgatcac ctgatcaacc acgccgcccg cctgcgccac cgcacccgtg gccggctgag
     1081 ctgcccgatg gtgctgcgca ccccggtggg cggcggcatc cacgccccgg aacaccacgg
     1141 cgaatccccc gaagcctggc tggcgcacac ccccggcctg aaagtggtca gccccagctc
     1201 acccgcccgc gcctacggcc tgctgctggc cgccatccgc gaccccgacc cggtgatttt
     1261 tttagagccc acccggctgt accggctgct gaaagaacca gtcgctgaca acggcgaagc
     1321 cctgccgctg ggcgcggcct tcaccctgcg cgaaggctgc gacgtcaccc tggtcagctg
     1381 gggcgcctcg gtgctggaaa ccctgcaagc cgccgagcaa ctggccgaac agggcgtatc
     1441 cgccgaagtg atcgacgtgg ccagcctgaa accgctggac atgcccacca tcctcgcctc
     1501 ggtggccaaa accggccgct gcgtgatcgt gcacgaagcc gcccgccaat gcggcgtcgg
     1561 cgccgaaatc gccgccaacc tggccagcga aggcctgatg agcctgctcg ccccggtcga
     1621 acgcgtcacc ggctttgaca ccccgctgcc cctggcccgg ctggaagacg aatacctgcc
     1681 cagcgtcgcc cgcatcctgg cggcggttga gcgcgtgcgg gcctactaaa agcaaacaca
     1741 tggatcaata cgctggacat ggcatgcaaa ggcattgaat cgcgcaaccc gtcgcgccta
     1801 gccggtttgc cccccataaa gcgcgccgag catcgcagcc agccaggggg accccgtcgt
     1861 ccgacccggc taagcggcgg cgcagcccat tatgatcacc actgcccatg cccagctcca
     1921 cacaggaaac ccaacaccat gaaaaccttc aaactccccg acctgggcga aggtctgcaa
     1981 gaagcggaaa tcgtcgcctg gcacgtcaac gctggcgacc acatcgtcgt cgatcaaccg
     2041 ctgctctccg tcgaaaccga caaagccatc gttgacgtgc cctcgccgtt tgccggcaag
     2101 gtgctcaccc tgcacgccaa agtcggcgat gtggtggcgc tggacggcgc gctggtggac
     2161 atcgacgacg gcggccaggt ggaagacagc ggctcggtgg ttggcgacgc cccgaccgcc
     2221 accactgccc atgaagtgcg cgaacacgcc agcgccgccg cccccgccgc acgcggcgtg
     2281 cgcgccgccc ctgccgtgcg cgcgctggcc agcaagctgg gcgtggattt gtccatcgtc
     2341 acccccagcg ggccggacgg cgtgatcctg gccagcgacg tcgagcgggt ggccaaggtg
     2401 ctggccaatg tcgccgcgct caccccgctg cgcggcccgc gtcgggccat ggcgcagaac
     2461 atggcgctgg cccacgccga agtggtgccc gtcaccttgg tggacgacgc cgacatccac
     2521 gcctgggcgg ctggcgaaga caccacctgg cgtttgctgc gcgccatggt ggccggctgc
     2581 cgcgccgcgc ccgcactgaa cgcctggttc gacagccagg cgctgggcgt gcgccagctc
     2641 agccagatcg acgtcggcat cgccaccgac accgaagaag cgctgtttgt gccggtgctg
     2701 cgcaatgtcg ccgagcgcag ccgcgaggat ttgcgcgccg cgctggagca gatgaagcaa
     2761 gacgtgcgtg cgcgcaccat tccgccggaa tcactgcgcg ggcacagcat tactttgtcc
     2821 aacttcggca tgtttgccgg ccgttacgcc agcccggtgg tgatgccacc gacggtggcg
     2881 attgtcggcg ctggccgcgc ccggcgcgag ccggtggcgg tgggcgacgc cgtggtggtg
     2941 catcgggtgc tgccgatgtc gctgaccttt gaccaccgcg ccgccaccgg ggcggaagcg
     3001 gcgcgctttc tggcggcgat gatggctgat ttgcagcggc cagactgagc gctggcggcg
     3061 gggtcagctg caaaaaaaac cggcgcactg ctgatcaggc agtgcgccgg ttttttttgg
     3121 gggggagcgg cgcttaatcc actcaggcca cggccggctg acgggtcagc agggtcagca
     3181 cttccgccat gcggcggcgc agttcgcggc gatcgacgat catgtcgatg gcgccttttt
     3241 ccagcaggaa ttccgcccgc tgaaagcctt ccggcagggt ttcgcgcacg gtttgctcga
     3301 tcacgcgcgg gccggcaaag ccaatcagcg cgcccggctc ggccatcacc acatcgccca
     3361 ggaaggcaaa gctggcggag acgccgccca tggtcgggtc ggtcagcacc gagatgaacg
     3421 gcagtttgtg gtcagtcagc agttgcagcg cggcgctggt tttggccatc tgcatcagcg
     3481 agctcaggcc ttcctgcatg cgcgcgccgc cggaggcgga tacgcagatg aacggcgcgc
     3541 gttgctcaac ggcggcgcgc acgccgcgca caaagcgctc gcccaccacc gaacccatcg
     3601 agccgccaat aaagcggaac tcaaacgcgg cggcgaccac cggcagattg tggatgctgc
     3661 cctgcatcac caccagggcg tcgtcttcgc cggtgctgct ttgcgcggcg gtgaggcgct
     3721 cggggtattt tttgctgtcc ttgaacttca ggatgtccac cggggtgact tcgctgccga
     3781 tttcaaagcg gccttcggcg tccagcagca tgtccagccg cgcgcgcgcg cccaccgcgt
     3841 tgtggtggct gcacttgggg cagacgtgca gattgttttc caggtcggtg tgatacagca
     3901 cggcctcaca ggccgagcac ttgctccaca gcccttccgg cacggcggat ttgccggttt
     3961 tgttgtcttt tttgatcttc gggggaagga gcttgttcaa ccagctcatg ttctctcctg
     4021 cgcccggccg tcgcgtcgtg ggcggcggcg ggctggggtc tatcgggctg agtccgggcg
     4081 ggccgggctc aggcgtccag ggcggcgcgg atgtcgcgca gcagggtttg caaggtggcg
     4141 gcggcttcgc cgggggcggc ggcttccacc gcctgcacca gccggctgcc aatcaccacg
     4201 gcgtcagaca catcggccac ctgacgggcg gtctggccat cgcggatgcc aaagcccacg
     4261 ccaatcggca gctggatgtg ctgacggatt tccgccaccc ggcgcgccac ttcagtgaca
     4321 tccaggttgg cggcgccggt gacgcccttg agcgacacgt aatacacata gccgcgcgcg
     4381 tggcgggcaa tgccggacag ccgctcggcc ggggtggtcg gggcaatcag gtaaatcggc
     4441 gccagttgtt cggcgtgcag cgccagcgac agcgcctcgg cttcttctgg cgggcaatcc
     4501 accgtcagca ggccatccac gccagcggcg cgggccgcct tggcaaactc ggcgtagccc
     4561 atggcttcca ccgggttcag gtagcccatc agcaccaccg gggtggcgtc gtcgtcggcg
     4621 cgaaactgcg ccaccatggc cagcacatcg cgcaggccca cgccgtgggc cagggcgcgc
     4681 tcgctggcgc gctggatcac cgggccgtcg gccatcgggt cggaaaacgg cacgcccagc
     4741 tcaatcacgt tggcgccgcc ggccaccagc gcgtgcatca tcgccacggt ctggcccggt
     4801 tcggggtcgc cggcggtaat gaacgggatc agcgccttgc gcccggccag ctcggcaaaa
     4861 cagcggtcaa tacggttcat gcatcatcca tcaattgcgc gcccggcgcg cggccgggct
     4921 gggttggctt gtcagagcgt gatgccggcc aggccggcca cggtgttgat gtctttgtcg
     4981 ccacggccgg acagattgac cagaatcacc tgatccttgc ccagggtggg cgccagcttg
     5041 gcggcgtagg ccagcgcgtg gctggattcc agcgcgggga tgatcccctc gtagcggcac
     5101 agatcgtgga aggcggtcag cgcctcggcg tcggtcaccg ccacgtattc ggcgcggccg
     5161 atgtccttca ggtagctgtg ctcggggccc acgcccggat aatccaggcc ggccgacacc
     5221 gaatgggttt cgatgatctg gccgttgtcg tcctgcatca gatagctgcg cgcgccgtgc
     5281 agcacgccag ggcggccggc cgacagcggc gcggcgtggc ggccgctgtc caggccgtcg
     5341 ccgccggctt ccacgccaat caggcgcacg ccttccaccg ggatatacgg gtagaagatg
     5401 ccgatggcgt tggagccgcc gcccacgcac gccaccacgg cgtccggctg gcggccgatc
     5461 atctcgggca tttgcacctt ggattcttcg ccaatcaccg agacaaaatc ccgcaccagc
     5521 atcgggtagg ggtgcgggcc ggcggcggtg cccagaatgt agaaggtgct gtccacattg
     5581 gtcacccagt cgcgcatggc ttcgttgagc gcgtccttga gggttttcga gccggattcc
     5641 accggcacca cggtggcgcc cagcagcttc atgcggaaca cattgggcgc ctgacgcttg
     5701 acgtcttcag cgcccatata caccacgcat tccatgccgt agcgggcggc cacggtggcg
     5761 gtggccacgc catgctggcc ggcgccggtt tcggcgatca cccgcttttt gcccatgcgc
     5821 ttggccagca gcgcctggcc gatggcgttg ttgatcttgt gcg
//
LOCUS       DAOPYX010000193         5846 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_16997_length_5846_cov_11.905198, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000193 DAOPYX010000000
VERSION     DAOPYX010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 5846)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5846)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5846
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16997_length_5846_cov_11.905198"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..299
                     /locus_tag="PKN55_09980"
     CDS             <1..299
                     /locus_tag="PKN55_09980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014088527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HNX71615.1"
                     /translation="LDWAAITRPQQTVVIYMGVSTAPALCAALIEHGKAADTPAAVVE
                     RATTRAQRVLTGTLTTLPGLIAEHQIQSPALIIVGDVVKLAGTLAWFDGAGRVG"
     gene            complement(459..722)
                     /locus_tag="PKN55_09985"
     CDS             complement(459..722)
                     /locus_tag="PKN55_09985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019923715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HNX71616.1"
                     /translation="MSAALACRDGCGACCIAPSISSPIPDMPAGKPAGVRCAQLDEHA
                     RCRIFSSPLRPAVCGGLQPQAEMCGEDQAYAMHWLTRLERLTG"
     gene            complement(719..1492)
                     /locus_tag="PKN55_09990"
     CDS             complement(719..1492)
                     /locus_tag="PKN55_09990"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: protein motif:HMM:NF001754.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HNX71617.1"
                     /translation="MIARPHAPTDASAALDTLAGWLASARHVAVLTGAGMSTESGIPD
                     FRSYQGRYTQNASLADVLSIDYFLHNPAAFWEAFKEIFELKLTGDKQPNAGHRFLAWL
                     AAQGKTVSVLTQNIDGLHQRAGSEQVVEVHGTLQQAVCPACGRVHDLAHVLAEPLPRC
                     LSCHTALKPDVVLYGEAVHRFDEALTHTLAADVLLVLGSSLEVGPINLLPLEAQRHGI
                     RCALINLDATRMDRCFDLILRAPIGQTAQALQARLEGGR"
     gene            complement(1489..1827)
                     /locus_tag="PKN55_09995"
     CDS             complement(1489..1827)
                     /locus_tag="PKN55_09995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014088810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HNX71618.1"
                     /translation="MPTPPLDLSPLQAQLRAFAQARGWQPHHTPRNLLLALVGEVGEL
                     AEILQWRSDAELAALAQDNPTEWEHLGEELADVQIYLARLADVLGVDMAAAVADKLDK
                     NARKYPEPQS"
     gene            complement(1952..3577)
                     /locus_tag="PKN55_10000"
     CDS             complement(1952..3577)
                     /locus_tag="PKN55_10000"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNX71619.1"
                     /translation="MRVFAQLRATLMAFAAVLCVLVALAGAAGLYGARLNHGHFDQLQ
                     AHRAVAEQITAINYKVFDSRLHIALALSERTPARQQKEAEVIHKNMQAMRDSQAALGK
                     LAMPAELAAPVQQFLTVVGAFSEAYLQPAEAAMRAADVARLEQLLSTQEERFYQPIKQ
                     GRETIQATQHAAAQALGQAAADSEQLAWRLAVLAIACALGLALSFSLLTIRRLNHQLG
                     ALGAAMGQLERQRDLTVRAGLAGGSELAELGRGLDAVLGSLGGVLRQVSQHAGAAQRA
                     GDTLQAEASQTEAIVARQNQAIDNATQALQQVVTQTHTLAAQFGHTVQLASDSENEGA
                     EGARMVSDVAATMREIAERVGASSADIRQLGEQSAQVDQIVATIQEITAQTNLLALNA
                     AIEAARAGEAGRGFAVVADEVRRLAERTQEATLEIQQTLERIRSETNQASDNMHISQA
                     RVEDGLSRANAAAQAIVGVRERLAGMRSSIDDIDQAIRAQERASEAVAASMGQAVEAS
                     AQTAERARATRAAADQVQALGGQLHAEVGRFVL"
     gene            complement(4564..>5846)
                     /locus_tag="PKN55_10005"
     CDS             complement(4564..>5846)
                     /locus_tag="PKN55_10005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3656 domain-containing protein"
                     /protein_id="HNX71620.1"
                     /translation="ALIDAGVRSFKIEGRYKDMDYVKNITAHYRLLLDEILARRPELA
                     RASSGECHISFTPNPDKTFHRGSTDYFATGRKGDIGAFDSPKFVGLPLGVVSRVHAHS
                     FELVCQDPLSNGDGLNFMKKRDVIGVQANTVQHLGADADGERWKITPNDPAVLAQLKP
                     GMQMHRNRDHAWEQALSKKSADRRVGVWLTLSDDADGLALTLTDEDGLSVTRHAPLAL
                     EPARDGERALASLREQLAKLGNSMFVARDITLRLRQPWFAPASLLNALRRDAVAELQA
                     LRVAQWPRPPRKTPVEPPAAYPETSLSYLANVYNHLARQFYTRHGVSLIDAAYEAHEE
                     AGEVSLMITKHCLRFSFNLCPKQAKGVKGVMGQVRADPMVLKNGNETYTLKFECRPCE
                     MHVMGKMKKHIFQSPPPVSLPAVAPVTFHPRRPD"
BASE COUNT          879 a         1851 c         2011 g         1105 t
ORIGIN      
        1 acctggactg ggccgccatc acccgcccgc agcaaaccgt ggtgatctat atgggggtgt
       61 ccaccgcccc ggcgctgtgc gccgcgctga tcgagcacgg caaagctgcc gacacccccg
      121 ctgccgtggt ggaacgcgcc accacccgcg cccagcgcgt gctgaccggc acactgacca
      181 ccctgcccgg attgattgcc gaacaccaga ttcaatcccc ggcgctgatt atcgtcggcg
      241 acgtggtgaa actggccggg accttggcct ggtttgatgg ggcggggcgg gtgggttaag
      301 cgctgttcac caaacgcgcc tggcgttgtt gcaagccgta cggtctgcgg tctgtgcgtc
      361 tggccaaggg cgctgtgctg agtgctgtga gcgacgctaa gccgtctttg cctgttgtgt
      421 cgggaagcca gcgcccgccc tgtgggcgca tggctgtact accccgtcaa ccgctccagc
      481 cgcgtcagcc agtgcatggc gtatgcctga tcttccccgc acatctccgc ctgtggctgc
      541 aaaccgccgc agaccgccgg gcgcagcggt gagctaaaaa tccggcagcg ggcgtgttcg
      601 tccagttgcg cgcagcgcac gccggccggt ttgcctgctg gcatgtcggg gatggggctg
      661 gagatggacg gcgcgatgca gcaggcgccg cagccgtcgc ggcaggccag cgccgcgctc
      721 atcggccgcc ttccagccgc gcttgcagcg cctgggcggt ttggccgatg ggggcgcgca
      781 ggatcaggtc gaaacagcgg tccatgcgcg tggcgtccag gttgatcagc gcgcagcgga
      841 tgccgtggcg ctgggcttcc agcggcagca ggttgatcgg gccgacttcc agcgacgagc
      901 ccagcaccag cagcacatcg gccgccagcg tgtgcgtcag cgcctcgtca aagcggtgca
      961 ccgcctcgcc gtacagcacc acgtccggct tgagcgcggt gtggcagctc aggcagcgcg
     1021 gcagcggctc ggccagcaca tgggccagat cgtgcacgcg gccgcaggcc gggcacaccg
     1081 cctgttgcag cgtgccgtgc acttccacca cctgttcgct gccggcgcgt tggtgcaggc
     1141 cgtcgatatt ctgggtgagc acgctcaccg tcttgccctg cgccgccagc caggccaaaa
     1201 agcgatggcc ggcgttgggc tgtttgtcgc cggtgagctt gagctcgaaa atctctttga
     1261 acgcctccca gaacgccgcc gggttgtgca aaaaatagtc aatcgacagc acatcggcca
     1321 ggctggcgtt ttgcgtgtag cgcccctgat acgaacgaaa atccgggatg ccggattcgg
     1381 tgctcatgcc ggcgccggtc agcaccgcca catggcgggc gctggccagc cagccggcca
     1441 gggtgtccag cgcggcgctg gcgtcggtgg gggcgtgagg gcgggcgatc atgattgggg
     1501 ctccgggtat ttgcgcgcgt ttttgtccag cttgtcggcc acggcggcgg ccatgtccac
     1561 ccccagcaca tcggccagcc gcgccagata aatctgcaca tcggccagct cttcgcccag
     1621 gtgttcccac tcggtggggt tgtcctgcgc cagcgcggcc agctcggcgt cgctgcgcca
     1681 ttgcaggatt tccgccaact cgccgacctc gcccaccagg gcaagcagca gattgcgcgg
     1741 ggtgtggtgc ggctgccagc cgcgcgcctg ggcaaaggcg cgcaactggg cttgcagcgg
     1801 agagagatca agcggtgggg tgggcatcac gggcgcggca gtcaaaaaaa cctattttac
     1861 cgcgccgggg ggtgaaggga gacggctggg gtcaagccgg gcgcaggctg agcctgcgca
     1921 acaacgccag gagggggttg ctgcgcggcg ctcacaacac aaagcgcccc acttcggcat
     1981 gcagctgacc gcccagcgcc tgcacctggt cggcggcggc gcgggtggcg cgggcgcgtt
     2041 cggcggtttg cgccgaggct tccaccgcct gacccatgct ggccgccact gcttcgctgg
     2101 cgcgctcttg cgcgcggatg gcctggtcga tgtcgtcaat gctgctgcgc atgccggcca
     2161 agcgctcgcg cacgccgaca atcgcctggg cggcggcgtt ggcgcggctc aggccgtctt
     2221 ccacccgcgc ctggctgatg tgcatattgt cgctggcctg gttggtttcg ctgcggatgc
     2281 gctccagggt ctgctggatt tccagcgtgg cttcctgcgt gcgctcggcc agccggcgca
     2341 cctcgtccgc caccacggca aagccgcgcc cggcttcgcc tgcgcgcgcc gcttcgatgg
     2401 cggcgttcag cgccagcaaa ttggtttgcg cggtgatttc ctgaatggtg gcgacaatct
     2461 gatccacctg ggccgattgc tcgcccaact ggcgaatgtc ggcgctggag gcgcccaccc
     2521 gctcggcaat ctcgcgcatg gtggccgcca catcgctgac catgcgcgcg ccctcggcgc
     2581 cttcgttttc gctgtcgctg gccagctgca cggtgtggcc aaactgcgca gccagcgtgt
     2641 gggtttgcgt caccacctgc tgcaaggcct gggtggcgtt gtcgatggcc tgattttgcc
     2701 gcgccacaat cgcctcggtc tggctggcct cggcttgcag ggtgtcgccc gcgcgctggg
     2761 cggcgccggc atgctggctg acctggcgca acaccccgcc caggctgccc agcaccgcat
     2821 ccagcccgcg ccccagctcg gccagctccg agccgcccgc caggccggcg cgcacggtga
     2881 ggtcgcgctg acgctccagc tggcccatgg ccgcccccag cgcgcccagc tggtgattca
     2941 gccggcggat ggtcagcagg ctaaagctca gcgccagccc cagcgcgcac gcaatcgcca
     3001 gcaccgccag ccgccaggcc aactgctcgc tatccgccgc cgcctgcccc agcgcttgcg
     3061 cggccgcatg ttgggtggcc tgaatggttt cgcggccctg tttgatcggc tgataaaagc
     3121 gctcttcctg cgtgctcagc aattgctcca gccgggccac atcggccgcg cgcatcgccg
     3181 cctcggccgg ctgcaaatac gcctcggaaa acgccccgac cacggtcaaa aactgctgca
     3241 ccggtgccgc cagctcggcc ggcatcgcca gcttgcccag cgccgcctga ctgtcgcgca
     3301 tggcctgcat gtttttgtgg atcacctcgg cttctttttg ctggcgcgcc ggcgtgcgct
     3361 cgctcagcgc cagggcaata tgcagccgcg aatcaaacac cttgtaattg atggcggtga
     3421 tctgctcggc caccgcccgg tgcgcctgca actggtcaaa atggccatga ttcagccgcg
     3481 ccccatacag ccccgccgcc ccggccaagg ccaccagcac acacaacacc gccgcaaacg
     3541 ccatcaacgt ggcgcgcaat tgcgcaaaca cccgcatgaa aaagcccccc gcaaaacacc
     3601 cgcgcccccg gcgcacatcg gacacccgcg ccagcatggg cggggatatg ccattgttcc
     3661 gcagggggct taattcatgc ttagtgaaag tgaagcggtc tgctcaacct gcccgcttgg
     3721 cctgcgccag cagcgcttgt gactcgtcat cgccataacg gctgatcagc acatccagcg
     3781 gccaggtgcg caccggctga cgctcggcca ggcgttggtc ttggaggatc cagttgcggg
     3841 gtttgcttag gtcgatgggc tgattggggt cgatggcgcg cagggtgcag cggcggtcga
     3901 gaaaatcttg cagtcgggcg ggccaggaga gggcgaggtt gtcggtttgt gaggcgaagt
     3961 atgtgtgaac cagttcgcca gtgctggcgt tccagaggcg ggtggtgctg tcggcgctgg
     4021 cggtgaggat gtggtggcca tcagggggga gtgtttgcgg atacagttgg cagttgggcg
     4081 tggccgaagg ccagggtgat tttggggctg gtggaggtgt tcatgctgaa tgggtggggc
     4141 cggtggataa gctgggcttt cctgggtgtg tctgtcgtgt aaaaaagtgg gggttttgcc
     4201 cgcttgatgc agtgggtcgg gaaatgggtg gagttttatg taatgtgtaa aaaatttatt
     4261 acatatctcg tgttgccgtg tcaggtggtg gtgacggggg tgtgtggatg atttatccac
     4321 aggtggataa gcgccgttca caaaacgctt ctggcgttgt tgcgcggctt ggtcgtactg
     4381 cttgttctgt ctgcggttgc gcggccaggc gtgctgtgcg aagttttttt acgacgcgca
     4441 gtcggggtga gcgcgatgcc tgtgggtaag ttatgcacag gcatcgcgcc cagtcatgcc
     4501 tgattgggct gtggatcatt tcagcatgtc cctgagctga tccacagctt gcgggcgctg
     4561 tgctcagtcc ggtcggcgcg ggtgaaaagt caccggcgcc actgccggca gcgacactgg
     4621 cggcggcgat tggaagatgt gttttttcat tttgcccatc acgtgcattt cgcacggccg
     4681 gcattcaaat ttcagcgtgt aggtttcatt gccgtttttc agcaccatcg ggtcggcgcg
     4741 cacctggccc atcacgcctt tcacgccctt ggcctgtttc gggcacagat tgaaggaaaa
     4801 gcgcaggcag tgtttggtga tcatcaggct gacttcgccg gcttcttcgt gcgcttcgta
     4861 agcggcgtcg atcaggctga cgccgtggcg ggtgtaaaac tggcgggcca ggtggttata
     4921 cacattggcc aggtagctga gcgaggtttc cgggtaggcg gcgggcggct ctaccggggt
     4981 tttgcgcggc gggcgcggcc attgcgccac gcgcagcgct tgcagctcgg cgacggcgtc
     5041 gcggcgcagg gcgttcagca gcgaggccgg ggcaaaccag ggttggcgca ggcgcagggt
     5101 gatgtcgcgg gcgacaaaca tgctgttgcc cagcttggcc agttgttcgc gcaggctggc
     5161 cagcgcgcgc tcgccgtcgc gggccggctc cagcgccagc ggcgcatggc gggtgacgct
     5221 caggccgtct tcgtcggtca gcgtcagcgc caggccgtcg gcgtcgtcgc tgagggtcag
     5281 ccacacgccc acccggcgat cggcggattt tttgctcagc gcttgttccc aggcgtggtc
     5341 gcggttgcgg tgcatttgca tgccgggctt gagctgggcc agcacggccg ggtcgttggg
     5401 ggtgattttc cagcgctcgc cgtcggcgtc ggcgcccagg tgttgcacgg tgttggcctg
     5461 cacgccgatg acgtcgcgtt ttttcatgaa gttcaggccg tcgccattgc tcagcggatc
     5521 ctggcacacc agctcaaagc tgtgggcgtg cacccggctg accacgccca gcggcaggcc
     5581 gacgaatttg ggcgaatcaa aggcgccaat atcgcctttg cggccggtgg caaagtaatc
     5641 ggtgctgccg cgatgaaagg ttttgtccgg gttgggggta aagctgatat ggcattcgcc
     5701 gctggaggcg cgcgccagct cggggcggcg ggccagaatc tcgtccagca gcaggcggta
     5761 atgggcggtg atgtttttca catagtccat gtccttgtag cggccttcga tcttgaacga
     5821 gcgcacgcca gcgtcgatca gcgctt
//
LOCUS       DAOPYX010000194         5842 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_17014_length_5842_cov_14.815967, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000194 DAOPYX010000000
VERSION     DAOPYX010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 5842)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5842)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5842
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17014_length_5842_cov_14.815967"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..309
                     /locus_tag="PKN55_10010"
     CDS             <1..309
                     /locus_tag="PKN55_10010"
                     /inference="COORDINATES: protein motif:HMM:NF013777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNX71621.1"
                     /translation="LKRDIKRVTQKVLTQTLRKLERDGLVSRTVHATAPVTVEYALTP
                     LGQTLTDTVSALTHWAELNMDAVLAAQTAYDSAAQQAAVQAASVHRMGARALSPTSPA
                     "
     gene            489..1043
                     /locus_tag="PKN55_10015"
     CDS             489..1043
                     /locus_tag="PKN55_10015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012696245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HNX71622.1"
                     /translation="MSKTVVVYFSGYGHTQRVAQAVAEGAGAQLIAVDAEGNVPDSAW
                     DELAAADAIIFGAPTYMGSVPWQFKKFADASSKAWFTRAWQDKVFGGFTVSASLNGDK
                     QVTLINLQTLASQHGGIWVSLGLMPSNTLNASRNDVNNLGGSVGLLVQAPSDAGAEAI
                     PDGDIDTAKRYGARVTEVAMRLKG"
     gene            complement(1087..3579)
                     /locus_tag="PKN55_10020"
     CDS             complement(1087..3579)
                     /locus_tag="PKN55_10020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018152766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HNX71623.1"
                     /translation="MNAISDISHRVIDDHAVLDASADAVGGKAWQLAQLRRYGLPVAE
                     FIVLPAAAAAPTDEAEHAWAAVQARGWADKPLAVRSSAVGEDGAHASFAGIHRSCLNV
                     CGAAQLAEAIAAVRASLLSDSARAYRQRLGIAGQARMAVIIMPLLPAVAAGIAFTCDP
                     RDGREDRMLIHAHWGLGEALVGGEADGDEYLLAEDATDSWRVLSRRLGGKAQQRRCAD
                     GGGTELRATPAGQTSQPVLDTAQIETLAALLRDAAYALDFVAPFYDLEWLWDGARFWL
                     TQARPVTRRPHYTYPALHAQPAIWTRGNTCELLPDPLAAFDWNFSRRGVNALLEQGAR
                     LAGYPLQDGVQRAGLFHGRLYLEASIMQWELWNAVDVPPASMNVMMGGHQPEITVPAS
                     TWRERLRRGLNMLRYLRRAGAVRQRGEAAIVRVRAMAQRLRAAPPPAEHAALRAAIQE
                     AGQVARGEFDMFFLQGSGGGSLSLLRDTLEKAFPGEGDALGAALLAGGEASVTVQQNY
                     ALLALAQRARQEGRESTGFQRALADFMQAYGHRGHYETYFRSASWREQPDSLLAQLDS
                     LADIDADALRQRQQLAAAQAWARIRQHAPWPTRLLLRWLARAANRECNQREAARSALI
                     DNLDAVRHLADGAIALLRQRGVLHADEGRDALQHLFLWEIDSACQGSLPAASLRARLL
                     DRQARFARWQAETAPEYLLIQPDGQHTAGALPTPTAPSDGQRWRGLATGAGVARGRVR
                     RIRHPAEGVALQAGEILLAPATDPGWTPLFLKAGGLVVETGGYLSHAAIVAREFALPA
                     VVNLPGIMEALQDGEWVEVDGGRGVVRRVGNG"
     gene            complement(3576..4187)
                     /locus_tag="PKN55_10025"
     CDS             complement(3576..4187)
                     /locus_tag="PKN55_10025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018152767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNX71624.1"
                     /translation="MNDISTELGRRERKRLQQLDHLADTAWALFEAEGYEAVTMERIA
                     AEADVAKATLYKHFPVKEALLRHRFHRELRDSVSQMGAVMAAEPVGAARLAKFFALSA
                     AWSEAHRAYLLPYVQYRLAERRCPDDEAQRSGLERIFTALIAEGQAAGQFRADMGAPA
                     LAVYLEFLYLAALLRWLTGQAPALQAEFDTMLMLFLGGLEVRA"
     gene            complement(4184..5224)
                     /locus_tag="PKN55_10030"
     CDS             complement(4184..5224)
                     /locus_tag="PKN55_10030"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HNX71625.1"
                     /translation="MTTTISLAGNKYTSYIFIRRIHTGEAIMSRTLTLAVLQPEAVSH
                     SLTDSLDWLAKQAEQAAACGAQLALLPELFASPFWFTAEVWRWAQPQGGLVERFLQDT
                     ARANGLYLGGSYLEARGEHFYNTFALASPAGQIVGRVGKAHPCSLERAVFAPSPGAQV
                     IDTELGRIGVAICYDNALREVVDPLLAAAPDVWLMPLSAPMPPASLGGQRGLEAYLTI
                     LRDSPAAMAALFGLPVAMANKCGRWQSPMPGWLPAADSHFPGLSCIADSDGVQVAALG
                     AQPGFAVAQVTLDPARKRCQVPAAYQRHRPWLVAPPLDYRLFALFEWWGARHYRRHPQ
                     RAALAQQQSLCT"
     gene            5569..>5842
                     /locus_tag="PKN55_10035"
     CDS             5569..>5842
                     /locus_tag="PKN55_10035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HNX71626.1"
                     /translation="MRTSIRLMALAVSSALLAAPAMADTLQKIKDTGTITIGHRESSI
                     PFSYLDGNQKPVGYSMDLCNKIVDAVKKELKMPALVTKLTPVTSQTR"
BASE COUNT          958 a         1942 c         2001 g          941 t
ORIGIN      
        1 ctcaagcgcg acatcaagcg cgtcacgcag aaagtgctga cgcaaaccct gcgcaagctg
       61 gagcgcgacg gcctggtcag tcgcacggtg cacgccaccg cgccggtgac ggtggaatac
      121 gcgctgaccc cgctggggca aacgctgacc gacaccgtct cggcgctgac ccactgggcc
      181 gaactgaata tggacgccgt gctggcggcg caaaccgcct acgacagcgc cgcccaacag
      241 gcagcggtgc aggccgccag cgtgcatcgc atgggcgcgc gggcgctgtc gcccacatca
      301 ccggcctgac cgcgcggtat gggccagcgt gcgcctggtg gtttccttgg ggaaacctga
      361 gcacctgaaa gtgcctcatt gaacacttgg tttctgctgg atactatggt tgccacacct
      421 cgcctgctgt cgggcgcggg ttttttctct ctccgggcgc agcggcccga ccagtcaagg
      481 acaaggcaat gagcaaaaca gtcgtggtgt atttttccgg ttatggccac acccagcgcg
      541 tggcgcaagc cgtggcagag ggcgcgggcg cgcagttgat tgcggtggac gccgaaggca
      601 atgtgccgga ttcggcctgg gatgagctgg ccgccgccga cgccatcatc tttggcgcgc
      661 ccacctatat gggcagcgtg ccctggcagt tcaaaaagtt tgccgacgcc agctcgaaag
      721 cctggttcac ccgcgcctgg caagacaagg tgtttggcgg cttcaccgtc agcgccagcc
      781 tgaacggcga caagcaagtc accctgatca acctgcaaac cctggcctcg cagcacggcg
      841 gcatctgggt cagcctgggc ctgatgccgt ccaacacgct gaacgccagc cgcaacgacg
      901 tgaacaatct gggcggctcg gtcggcctgc tggtgcaagc cccgtccgat gctggcgccg
      961 aagcgattcc tgatggcgac atcgacaccg ccaagcgcta cggcgcgcgg gtgactgagg
     1021 tggctatgcg tttgaagggc tgaggattgt tctcctggcg gcaggtgttc cttcttgcgc
     1081 ctgccgttaa ccgttgccca cccgccgcac caccccgcgc ccgccgtcca cctccaccca
     1141 ttccccatct tgcagcgcct ccataatgcc cggcaggttc accactgccg gcagggcgaa
     1201 ttcgcgggcg acgatggcgg cgtgggagag atagccgccg gtttccacca ccaggccgcc
     1261 ggccttgaga aacagcgggg tccagccggg gtcggtggcg ggggccagca ggatttcgcc
     1321 ggcttgcagc gccacgcctt cggccgggtg gcggatgcgg cgcacgcggc cgcgcgccac
     1381 gccagcgccg gtggccaggc cgcgccagcg ctggccgtcg ctgggcgcgg tgggggtggg
     1441 cagcgcgccg gcagtgtgtt ggccgtcggg ctggatcagc aggtattccg gcgcggtttc
     1501 ggcttgccag cgggcaaagc gcgcctggcg atccagcagc cttgcgcgca ggctggcggc
     1561 gggcaggctg ccctgacagg cgctgtcgat ttcccacaga aacagatgtt gcaaggcgtc
     1621 gcggccttcg tcggcgtgca gcacgccgcg ctggcgcagc agggcaatcg cgccgtcggc
     1681 caggtggcgc acggcgtcca ggttgtcgat cagcgcgctg cgcgcggctt cgcgctgatt
     1741 gcattcgcgg ttggcggcgc gcgccagcca gcgcagcagc aggcgggtgg gccacggcgc
     1801 gtgttggcgg atgcgcgccc aggcttgcgc ggcggccagt tgctggcgct ggcgcagcgc
     1861 gtcggcgtcg atgtcggcca ggctgtccag ctgggccagc aggctgtcgg gctgttcgcg
     1921 ccagctggcg ctgcgaaaat aggtttcgta atggccgcga tggccatagg cctgcataaa
     1981 atccgccagc gcgcgctgaa accccgttga ctcacggcct tcctgccgcg cccgttgcgc
     2041 cagcgccaac aaggcgtagt tttgctgcac ggtgacgctg gcttcgccgc cggccagcag
     2101 cgccgcgccc agggcgtcgc cttcgccggg aaaggctttt tccagggtgt cgcgcagcaa
     2161 tgacaggctg ccgccgcccg agccctgcaa gaaaaacatg tcgaactcac cgcgcgccac
     2221 ctggccggcc tcctgaatgg cggcgcgcag cgcggcatgt tctgctggcg gcggcgcggc
     2281 gcgcaggcgc tgggccatgg cgcgcacgcg gacaatggcc gcctcgccgc gctggcgcac
     2341 ggcgccggcc cgacgcagat aacgcagcat attcagccca cggcgcaggc gctcacgcca
     2401 ggtggacgcc ggcacggtaa tttctggctg atggccgccc atcatcacgt tcatcgacgc
     2461 cggcggcacg tccaccgcgt tccacagctc ccattgcatg atgctggctt ccagatacag
     2521 gcggccatga aacagcccgg cgcgctgcac gccgtcttgc agggggtagc cggccagccg
     2581 cgcgccttgc tccagcaggg cgttgacccc gcgccgggag aaattccagt caaacgccgc
     2641 cagcgggtcg ggcagcagct cgcaggtgtt gccgcgcgtc cagatcgccg gctgggcgtg
     2701 cagcgccggg taggtgtagt gcgggcggcg ggtgaccggc cgggcctggg tcagccaaaa
     2761 gcgcgcgccg tcccatagcc attccaggtc gtaaaacggc gcgacaaaat ccagcgcgta
     2821 agcggcgtcg cgcagcagtg cggccagggt ttcgatttgg gcggtgtcca gcacgggctg
     2881 gctggtttgc ccagccgggg tggcgcgcag ttcggtgccg ccgccgtcgg cgcagcgccg
     2941 ttgttgcgcc ttgccgccca gccggcggct gagcacgcgc cagctgtcgg tggcgtcttc
     3001 cgccagcagg tattcgtcgc cgtcggcctc gccgcccacc agcgcctcgc ccagccccca
     3061 gtgggcatga atcagcatgc ggtcttcgcg gccgtcgcgc gggtcgcagg taaaggcgat
     3121 gccagccgcg actgccggca gcagcggcat gatgatcacc gccatgcgcg cttggccggc
     3181 aatgcccagc cgctggcggt aggcgcgggc gctgtcgctc agcaaagagg cgcgcacggc
     3241 ggcaatcgcc tcggccagtt gtgcggcgcc gcagacattc aggcagctgc ggtgaatgcc
     3301 ggcaaacgag gcgtgcgcgc cgtcttcgcc gacggcggac gagcgcaccg ccagcggctt
     3361 atccgcccag ccgcgcgcct gcaccgccgc ccaggcgtgt tcggcttcat cagttggcgc
     3421 ggcggcggcg gcaggcaaaa caataaactc ggccaccggc aggccatagc ggcgcagctg
     3481 cgccagttgc caggctttgc cgcccactgc gtcggcgctg gcgtccagca cggcgtggtc
     3541 gtcgatcacc cgatgggata tgtcagatat ggcgttcatg cgcgcacctc cagtccaccc
     3601 agaaacaaca tcagcatggt gtcaaattcg gcctgtagcg cgggcgcctg gccagtcagc
     3661 cagcgcagca gcgcggccag ataaagaaac tccagataca ccgccagcgc gggcgcgccc
     3721 atgtcggcgc gaaactggcc ggcggcctgg ccttcggcaa tcagcgcggt aaagatgcgc
     3781 tccagcccgc tgcgctgggc ttcgtcgtcc gggcagcgcc gctcggccag ccggtattgc
     3841 acatagggca gaagataggc gcggtgcgcc tcggaccacg ccgcgctcag ggcaaagaac
     3901 ttggccagcc gcgccgcgcc caccggctcg gccgccatca ccgcgcccat ctggctgacg
     3961 ctgtcgcgca gttcgcggtg aaagcggtgg cgcagcaggg cttctttgac cggaaagtgt
     4021 ttatacagcg tggccttggc cacatcggcc tcggcggcga tgcgctccat ggtcaccgcc
     4081 tcgtagcctt cggcttcgaa tagcgcccag gcggtgtcgg ccaggtggtc gagctgttgc
     4141 aggcgcttgc gttcgcggcg gcccagctcg gtgctgatgt cgttcatgtg cataagctct
     4201 gttgttgcgc cagggcggcg cgttgcgggt ggcggcggta atggcgcgcg ccccaccatt
     4261 cgaatagcgc aaacaagcgg taatccagcg gcggggccac cagccacggg cggtggcgct
     4321 ggtatgccgc cggcacctgg cagcgtttgc gcgccgggtc gagggtgact tgcgccacgg
     4381 caaaaccggg ctgggcgccc agcgccgcca cttgcacacc atcgctgtcg gcaatgcagc
     4441 tcaaacccgg aaaatgcgaa tccgccgccg gcagccagcc tggcatcggc gattgccagc
     4501 gcccgcattt attggccatc gccaccggca ggccaaatag cgccgccatg gcggcggggc
     4561 tgtcgcgcag gatggtcagg taggcctcca ggccgcgctg gccgcccagc gaggccggcg
     4621 gcatcggcgc gctcagcggc atcagccaca catccggcgc agcagccagc agcgggtcca
     4681 ccacttcgcg cagggcgttg tcgtagcaga tggccacgcc gatgcgcccc agctcggtgt
     4741 cgatcacctg cgcgccaggc gagggggcaa acaccgcgcg ctccagcgag cagggatgcg
     4801 ccttgcccac ccggccgaca atctggccgg ccggcgaagc cagggcaaag gtgttgtaaa
     4861 agtgctcgcc gcgcgcttcc agataactgc cgcccagata caggccgttg gcgcgagcgg
     4921 tgtcctgcaa aaagcgctca accagcccac cctgcggctg cgcccagcgc cacacttcgg
     4981 cggtaaacca aaacgggctg gcgaacagct cgggcagcag cgccagttgc gcgccgcagg
     5041 cggcggcttg ctcggcctgc ttggccagcc aatccaggct gtcggtcaga gaatgggaga
     5101 cggcttctgg ctgtagtact gccagggtaa gcgtgcgtga catgatggct tcaccagtat
     5161 gtatacgacg gataaaaata tacgacgtat atttattgcc ggcaagggaa atcgtggtgg
     5221 tcatctggcc ctgccaggcg acgcgggcgc gtgggccgtg atatggtttg ttcggttttc
     5281 cgaacggcga acagggggct gttcgggctg ccggacggcg cgggtgagat tggttggtga
     5341 aaaatcatgt caatcatgag tttgcgtgtt tttgcggcgg gcggattgcg ctggcccgct
     5401 ttctgctaat tcagtgtggc gagatggata aggcagccgc tgccgcccga cgcaacttgg
     5461 gcgtgcggca ttgagctgct accttgaatc atggcgccat cgcctctgtg cgatggccgc
     5521 cggaatttga cgcatggcgt gggccatgtg catgcacagg agatttagat gcgcacttcg
     5581 atccgtttga tggctctggc tgtttcttcc gcgctgttgg ccgccccggc catggccgac
     5641 accctgcaaa agatcaagga caccggcacg atcaccatcg gccatcgtga atcgtcgatt
     5701 ccgttctcct acctggacgg caatcaaaag ccggtgggct attcgatgga tctgtgcaac
     5761 aagatcgtcg atgcggtgaa aaaagagctg aaaatgccgg cgctggtgac caagctcacc
     5821 ccggtgacct cgcaaacccg ca
//
LOCUS       DAOPYX010000195         5814 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_17134_length_5814_cov_10.614169, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000195 DAOPYX010000000
VERSION     DAOPYX010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 5814)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5814)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5814
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17134_length_5814_cov_10.614169"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(2..1030)
                     /gene="galE"
                     /locus_tag="PKN55_10040"
     CDS             complement(2..1030)
                     /gene="galE"
                     /locus_tag="PKN55_10040"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018152849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HNX71627.1"
                     /translation="MNADTKPWVLVTGGAGYIGAHTCVALLEAGYPVLVFDSLVNGST
                     EALARASALGGGEIAFVQGDVRDRAALDALFARYPVSAVLHLAGVKAVGESVARPLYY
                     HEVNVAGTLTLAQAMHAADVRTLVFSSSATVYGAPARMPIDESAPLAPNNPYGRSKRM
                     AEDLLRDHCAAHPEWRLAILRYFNPVGAHPSGQLGEDPLGTPDNLMPFVSQVAIGRRP
                     ELAVFGNDYPTPDGTGVRDYLHVCDLARGHLAALHALPAAGGELTVNLGTGRGHSVLD
                     VVRAFETASGQTVPLRFAPRRPGDIAACWADPSRAEQLMGWRAECDLDAMCRDVWRWQ
                     RGNPEGYR"
     gene            complement(1027..1821)
                     /locus_tag="PKN55_10045"
     CDS             complement(1027..1821)
                     /locus_tag="PKN55_10045"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: protein motif:HMM:NF003380.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HNX71628.1"
                     /translation="MAKFLQRRRSRDSGPALPDVRVAEERGIRSLYLGSDTIQSSMRL
                     SDPTELVLTYTHAMMGFLLCLPSAPAAILHIGLGGGSLPRFCHARLPDTRNIAVELNP
                     EVIAVARSLFALPEDERLRVIQGDGVQFVGERTAEFDAVLVDAFDGHDLIAEMVAEPF
                     LHDCRQTLTENGVLVMNLWGNHPHYRRHVDCLRQAFDGRVLTLPAASHGNVAALALAG
                     PAGHRLRLDALEARARQLTADVELDFMAMVAALKRDNPHTASRLLP"
     gene            complement(1814..2782)
                     /gene="ttcA"
                     /locus_tag="PKN55_10050"
     CDS             complement(1814..2782)
                     /gene="ttcA"
                     /locus_tag="PKN55_10050"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011137678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HNX71629.1"
                     /translation="MNSPTPALDADALKAEQKAQYNANKLTKRLRHHVGDAINDFNMI
                     EPGDRVMVCLSGGKDSYALLDILLGLQKSAPIDFSLVAVNLDQKQPGFPEHVLPAYLD
                     SLGIEYRIVEEDTYSIVKRVIPEGKTTCSLCSRLRRGILYRVADELGATKIALGHHRD
                     DILQTLFLNMFYGGKLKAMPPKLLSDDGKHMVIRPLAYCREKDLERYAEQREFPIIPC
                     NLCGSQPNLQRQVVKEMLNDWDRRFPGRIETMFRSLQHVVPSHLADPTLFNFAGLALG
                     GALPDEGGDTAFDREEFHDPQPDADDRPARPVISILDSRPKDSACG"
     gene            complement(2856..3380)
                     /locus_tag="PKN55_10055"
     CDS             complement(2856..3380)
                     /locus_tag="PKN55_10055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX71630.1"
                     /translation="MATPHHQTFDRLVNDQTGFVGRVAYTFYKNDKLAWIRGFHDKHG
                     RAPSDEELALYFHIGIDQARLDAYLAEAERTLNEFIDLTASEEIRRGIEAYQQSDVVK
                     RCENILNGSKKTTWQAVKESLLSSVLSSFIITGLSVLLYLGSVAVFDDFRGLIHRLTA
                     PNPAGSSTQPSSPP"
     gene            complement(3389..3577)
                     /locus_tag="PKN55_10060"
     CDS             complement(3389..3577)
                     /locus_tag="PKN55_10060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX71631.1"
                     /translation="MRATVVSRESIRRANEAMKQHKTIADYQLPPAVASTLERSHYSM
                     AELNHHFSQVASSPFKKD"
     gene            complement(3704..4456)
                     /locus_tag="PKN55_10065"
     CDS             complement(3704..4456)
                     /locus_tag="PKN55_10065"
                     /EC_number="1.5.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012695771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pteridine reductase"
                     /protein_id="HNX71632.1"
                     /translation="MSPMDSPVALITGAARRIGAAIARTLHSQGWRVALHYRGSRQHA
                     DALAAELNQQRADSACCLQADLLDTAALPGLVAAAAARWGRLDALVNNASSFFPTPLG
                     QISQADWDDLIGSNLKAPLFLAQAAAPHLAAQGGAIVGLIDIHAERPMPGHTVYNLAK
                     AGHAQLIRALALELAPAIRVNGVAPGANVWPEGESVFDPAARAHILSQIPLQRVGEPE
                     DLAKVVAFLLSPAAGYVTGQILAVDGGRSIVQ"
     gene            4569..5726
                     /locus_tag="PKN55_10070"
     CDS             4569..5726
                     /locus_tag="PKN55_10070"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012695772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HNX71633.1"
                     /translation="MSALPLPDADAQAQSQQLSAQLRQQIAAAGGWLPFDQFMHHALY
                     APGLGYYSAGSRKFGPAGDFVTAPEMTPLFAQSLANTVAAVLTATGGDVLEFGAGSGR
                     LALDILLALEARGQLPAHYWILEVSPDLAERQRATLAAGAPHLLARVGWLSALPQGFR
                     GVMLGNEVLDAMPCKLLHWQDGVWQERGVACEDDGLFVWRDAPLAEPALAAAIAAHEP
                     PDNYLSEVQPAAAAFVRSLADCVDSAVLLLIDYGFPASEYYHPQRHRGTLMCHYRHHS
                     HDDPFFWPGLQDITTHVDFSAIWAAADAAGWQLEGYASQAGYLLDAGLLQALQALEPG
                     TPEYFQAAAMAQKLVSPAEMGELFKVIAFSRHLALDALLPGFAREDRSGEL"
BASE COUNT          979 a         1773 c         2015 g         1047 t
ORIGIN      
        1 cctacctata cccctccgga ttcccccgct gccagcgcca cacatcccgg cacatggcat
       61 ccagatcaca ctccgcccgc cagcccatca gctgttcggc gcggctgggg tcggcccagc
      121 aggcggcgat gtcgccgggg cggcgggggg cgaagcgtag cggcacggtt tggccgctgg
      181 cggtttcgaa ggcgcgcacc acgtccagca ccgagtgtcc acggccggtt cccaggttga
      241 cggtgagttc gccgcctgcg gctggcaggg cgtgcagggc ggcgaggtgg ccgcgcgcca
      301 ggtcgcagac gtgcaggtag tcgcgcacgc cggtgccgtc cggcgtgggg tagtcgttgc
      361 cgaacaccgc cagttccggg cggcggccaa tggccacttg gctgacaaag ggcatgagat
      421 tgtccggcgt gcccagtggg tcttcgccca gttggccgga ggggtgggcg ccgactgggt
      481 tgaagtagcg caggatggcc agccgccatt cggggtgggc ggcgcagtgg tcgcgcagca
      541 ggtcttcggc catgcgcttg ctgcggccat aggggttgtt gggggccagc ggcgcgcttt
      601 cgtcgatggg catgcgcgcg ggcgcgccgt acacggtggc ggaggagctg aacaccaggg
      661 tgcgcacgtc ggcggcgtgc atggcttgcg ccagggtgag ggtgccggcc acgttcactt
      721 cgtggtagta caagggccgg gcgacggatt cgcccacggc tttgaccccg gccagatgca
      781 gcacggcgct caccgggtag cgggcaaaca gcgcgtccag cgcggcgcgg tcgcgcacat
      841 cgccctgcac aaaggcgatc tcgccgccgc ccagcgcgct ggcgcgggcc agcgcttcgg
      901 tgctgccgtt caccaggctg tcgaacacca gcacgggata gccggcttcc agcagcgcca
      961 cgcaggtgtg cgcaccgata tagccggcgc cgccggtcac cagcacccag ggttttgtgt
     1021 cagcgttcat ggcagcagtc tcgatgcagt gtgcgggttg tcgcgcttga gcgcggccac
     1081 catggccata aagtccagct cgacatcggc ggtcagctgg cgggcgcggg cttccagcgc
     1141 gtccagccgc agccggtggc ccgccgggcc ggccagcgcc agcgcggcga cattgccgtg
     1201 cgaggcggcc ggcagggtga gcacgcggcc gtcaaatgcc tggcgcaggc agtccacatg
     1261 gcggcggtaa tgcgggtggt tgccccacag attcatcacc agcacgccgt tttcggtcag
     1321 cgtttgccgg cagtcgtgca aaaacggctc ggccaccatc tcggcaatca ggtcgtggcc
     1381 gtcaaaggca tccaccagca cggcgtcaaa ttcggcagtg cgctcgccca caaactgcac
     1441 gccgtcgccc tgaatcacgc gcaggcgttc gtcttctggc agggcaaaca ggctgcgcgc
     1501 cacggcgatg acttccgggt tgagttcgac ggcgatattg cgggtgtccg gcagccgggc
     1561 gtggcaaaag cgcggcaggc tgccgccgcc caggccaatg tgcaggatgg cggccggcgc
     1621 gctgggcagg cacagcaaaa agcccatcat cgcgtgggtg taggtcagca ccagttcggt
     1681 ggggtcggac aggcgcatcg acgactgaat ggtgtcgctg cccaggtata gcgagcggat
     1741 gccgcgttct tccgccaccc gcacatcggg cagcgccggg ccgctgtcgc ggctgcggcg
     1801 gcgttgcaaa aacttagcca caggcgctgt cctttggccg cgaatccaga atgctgatca
     1861 ccgggcgcgc cgggcggtcg tcggcgtcgg gctgcgggtc gtgaaactct tcacggtcaa
     1921 acgcggtgtc gccgccttcg tccggcagcg cgccgcccaa ggccaggccg gcaaagttga
     1981 acagcgtggg gtcggccagg tgcgagggca ccacatgttg caggctgcgg aacatggttt
     2041 cgatccgccc cggaaagcgc cggtcccagt cgttgagcat ctctttgacc acctggcgtt
     2101 gcagattggg ctgcgagccg cacagattgc acgggatgat ggggaattca cgctgctcgg
     2161 cgtagcgctc caggtctttt tcccggcagt aggccagcgg gcggatcacc atatgcttgc
     2221 cgtcgtcaga cagcagcttg ggcggcatgg ccttgagctt gccgccgtag aacatattca
     2281 ggaacagcgt ttgcaggatg tcgtcgcggt gatggcccag ggcaatcttg gtggcgccca
     2341 gctcgtcggc cacccggtac aaaatgcccc ggcgcagccg cgagcacagg ctgcacgtgg
     2401 ttttgccttc gggaatcacc cgcttgacga tgctgtaggt gtcttcttcg acaatgcggt
     2461 attcaatgcc caggctgtcc agataggccg gcagcacatg ctcgggaaag cccggctgct
     2521 tttggtcgag gttgaccgcc accagcgaaa aatcaatcgg cgccgatttt tgcaggccga
     2581 gcaggatgtc cagcagggcg tagctgtcct tgccgccaga caggcacacc atcacccggt
     2641 cgcccggctc gatcatattg aagtcgttga tggcgtcgcc cacatggtgg cgcagacgct
     2701 tggtcagctt gttggcgttg tactgggctt tttgctcagc cttgagcgcg tcggcgtcga
     2761 gcgccggagt gggggagttc atgcgagggt tacgctaaca gacggtaaag gctggggatt
     2821 ttacctgaga atgggtgagg gtgggctgag cggggtcaag gaggcgagga aggttgggtc
     2881 gatgagccag ccggattcgg tgcggtgagg cgatggatca aaccgcgaaa gtcgtcaaat
     2941 acggccacgc tgcccaaata cagcaatacc gaaaggccgg tgatgataaa agaagacagc
     3001 acactggaca gcaaggattc tttgacagct tgccaggtgg tttttttgct gccgttcaga
     3061 atgttttcgc aacgcttcac cacatcagat tgttgatacg cttcgatgcc gcgacgtatt
     3121 tcctcactgg cggtgaggtc gatgaattca ttcagcgtcc gctcggcctc ggccagataa
     3181 gcgtcaagcc gggcttgatc aatgcctata tgaaagtaca aggccagctc ttcatcactg
     3241 ggcgcgcgtc catgtttgtc gtgaaagccg cgaatccagg cgagtttgtc atttttgtaa
     3301 aacgtatagg ccacccgccc gacaaacccg gtctgatcgt tgaccagtcg atcaaaagtt
     3361 tgatgatgtg gtgtggccat ggcaagcctc aatccttctt gaatgggctc gacgcaactt
     3421 gcgaaaagtg gtggttcagc tcggccatgg agtagtgcga gcgctccagg gtgctcgcca
     3481 ctgccggagg cagttgatag tccgctatgg ttttatgttg cttcatggct tcgttcgcgc
     3541 gtcgaataga ctcacggctg acgacggtgg cgcgcatcgg ccatctcctc gaataagtca
     3601 gaatatcagg atgttagtgc aagataccgg ctaaaacaag cacttagcgt cgctcacaga
     3661 attcagcgca gcgctcctgc cactatctct tagccttgcc gcgctactgc acaatactcc
     3721 gcccgccatc caccgccaga atctgcccag tcacataccc cgccgctggc gacagcaaaa
     3781 acgccaccac cttggccagg tcttcgggtt cgcccactct ttgcagcggg atttggctga
     3841 ggatatgcgc gcgggcggcg gggtcgaata ccgattcgcc ttcgggccag acattggcgc
     3901 cgggggcaac gccgttgacg cggatggccg gggccagttc cagcgccagg gcgcggatca
     3961 gttgggcgtg gccggctttg gccaggttgt agacggtgtg gccgggcatc ggccgttcgg
     4021 cgtggatgtc gatcaggccg acgatggcgc cgccttgggc ggccaggtgc ggggcggcgg
     4081 cctgggcgag aaacaagggc gctttgaggt tggagcctat caaatcatcc cagtcggcct
     4141 ggctgatctg gcccagcggg gtggggaaaa aactggaggc gttgttcacc agcgcgtcca
     4201 gccggcccca gcgcgcggcg gcggcggcca ccaggccggg cagggcggcg gtgtccaata
     4261 ggtcggcttg caggcagcag gcggaatcgg cgcgttgctg gttgagttcg gcggccagcg
     4321 cgtcggcgtg ctggcgcgag ccccggtaat gcagcgccac gcgccagccc tggctgtgca
     4381 gggtgcgggc gatggcggcg ccgatgcggc gggcggcgcc ggtgatgagg gcgacggggg
     4441 aatccatggg cgacatctcc gaaaacaggc tgggcgcggg ggcgccgtcg gcaaagcagg
     4501 cgcgggcggc ttctcaaccc tggcaaattc gccgacaatc agcggcccac agtatacctg
     4561 acaccatcat gagcgccctg ccccttcccg acgccgacgc ccaggcgcaa agccagcaac
     4621 tgagcgcgca attgcgccag cagattgccg ccgctggcgg ctggctgccg tttgaccagt
     4681 tcatgcacca cgcgctgtac gcgccggggc tgggctatta cagcgccggc agccgcaaat
     4741 ttggcccggc gggcgatttt gtcaccgccc cagaaatgac ccccttgttt gcacaaagcc
     4801 tggccaatac cgtggccgcc gtgctgacgg ccactggcgg cgatgtgctg gagtttggcg
     4861 ccggctccgg ccggctggcg ctggatatct tgctggcgct ggaggcgcgc gggcagttgc
     4921 cggcgcatta ctggattctg gaagtctcgc cggatctggc cgagcgccag cgcgccacgc
     4981 tggctgccgg cgcgccgcac ctgctggcgc gggtgggctg gctgtcggcg ctgccgcagg
     5041 ggtttcgcgg ggtgatgctg ggcaatgagg tgctggacgc catgccatgc aagctgttgc
     5101 actggcagga cggcgtctgg caggagcgcg gcgtggcctg tgaggatgat gggctctttg
     5161 tctggcgcga tgcgccgctg gccgagccgg cgctggcggc ggcgattgcc gcgcatgagc
     5221 cgccggacaa ttatctgagc gaagtgcagc cggcggcggc ggcctttgtg cgcagcctgg
     5281 ccgactgcgt ggacagcgcc gtgctgctgc tgatcgacta tggctttccg gccagcgagt
     5341 attaccaccc gcagcgccat cgcggcacgc tgatgtgcca ttaccgccac cacagccacg
     5401 acgacccgtt cttctggccg ggtttgcagg acatcaccac ccatgtggat ttctccgcca
     5461 tctgggcggc ggccgacgcc gccggctggc agctggaggg ctacgcctcg caggcgggtt
     5521 atctgctgga cgccggcctg ttgcaggcgc tgcaagcgct ggagcctggc acgcccgagt
     5581 atttccaggc ggcggccatg gcgcaaaaac tggtcagccc ggcggaaatg ggcgagctgt
     5641 tcaaggtgat tgcctttagc cgccatctgg cgctggatgc gctgctgccg ggttttgcca
     5701 gagaggatcg ctcgggcgag ttgtgacgca gcaggttaca gttgtgtaat ttacatttta
     5761 cctgaatccg tggtcgtaaa acctcacaac cttcctgaag gccgaatcgc atga
//
LOCUS       DAOPYX010000196         5791 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_17231_length_5791_cov_9.189679, whole genome shotgun sequence.
ACCESSION   DAOPYX010000196 DAOPYX010000000
VERSION     DAOPYX010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 5791)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5791)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5791
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17231_length_5791_cov_9.189679"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..225
                     /locus_tag="PKN55_10075"
     CDS             <1..225
                     /locus_tag="PKN55_10075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX71634.1"
                     /translation="VLTAGPAAQLTLAQLRDLGVRRISTGSALARAAFTALHHAADDM
                     LNKGNFAYARTALSGEVLDGVMGKDASVFI"
     gene            complement(325..1869)
                     /locus_tag="PKN55_10080"
     CDS             complement(325..1869)
                     /locus_tag="PKN55_10080"
                     /inference="COORDINATES: protein motif:HMM:NF016158.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cryptochrome/photolyase family protein"
                     /protein_id="HNX71635.1"
                     /translation="MAPPIRTVRLILGDQLNIRHPWFAQVDDSVLYLMAELHQEARYV
                     RHHWQKLLGFLLAMRQFADTLRAAGHRLHYVRIGDAASQLPLPEVLRQILTEHGAARF
                     EYQQPDEYRLDQQLLLFSQSLPQPGEACDSAHFYTRRDELAACFPGDTPLLMERFYRR
                     MRRTHGILLDGAAPLGGRWNFDHDNRKKYKGTPPVPPALSFARDVSALLAEITAAGVS
                     GFGQIDATAFPWPANRDEALAQLAYFCQHLLSHFGEYQDALHSEQAFLFHSRLSFALN
                     LKLISPAEVINAAVAQFQQQPAQISLAQIEGFVRQILGWREYVRGVYWKAMPEYSQLN
                     QLDNHHPLPQMFWTGHTRMRCLHLALTQSLRHAYAHHIQRLMVIGNFALLSQRHPDAV
                     DAWYLGVYIDAIEWVELPNTRGMSQYADGGLLASKPYVSSGSYINKMSNYCAGCAYQV
                     KARTGDDACPFNSLYWRFLDDKKAHFAGNPRMAMMLKLLADLPADELAAIRARAGEIM
                     ADPDRF"
     gene            complement(1881..2951)
                     /locus_tag="PKN55_10085"
     CDS             complement(1881..2951)
                     /locus_tag="PKN55_10085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HNX71636.1"
                     /translation="MKRTRDSARNGWFWLAGLTAFGWLVYLLTPMLSPFVAGAVLAYI
                     VAPLVDWLEHRRVGRTLGTLLVIVLLMLALACLLVLIVPMLINQGQALSLRLPGLVDW
                     AENTLSPWLRDTWGMTWHFDAATLKRELTSNLGALRGMLARAAPLITDQGLALFGYLS
                     NLLLLPLVLFYLVRDWQQFTRPLARLIPRRWYRDLGMLGDEIDDMLGQFLRGQLAVML
                     IMAGVYGGGLWLVGLESGFAIGVVAGLLVFIPYVGAAIGLTLATLAALLQFGGLGGLL
                     LVWLVFVVGQLLESMFITPKLVGERIGLHPLVVIFALLAFGQLFGFVGVMMALPVSAA
                     LVVVVKLAQARYLASRFYRRRR"
     gene            3096..4133
                     /gene="purM"
                     /locus_tag="PKN55_10090"
     CDS             3096..4133
                     /gene="purM"
                     /locus_tag="PKN55_10090"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003796228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HNX71637.1"
                     /translation="MTQASLSYRDAGVDIDAGDQLVENIKPFAKRTMRPEVLGDLGGF
                     GALVEISKKYKEPVLVSGTDGVGTKLKLAFDWNRHDTVGIDLVAMSVNDILVQGAEPL
                     FFLDYFACGKLDVPQATDVIKGIAAGCEQAGCALIGGETAEMPGMYPVGEYDLAGFAV
                     GVVEKTQVITGRDIVPGDVVLGLKSNGVHSNGYSLVRKILDRAQPELDAEFDNGKTLR
                     DAIIAPTRIYVKPLLALMQQLPVKGMAHITGGGITENTPRVLPANTVAQIDAGSWELP
                     KLFQWLQAQGNVDMQEMYRTFNCGIGMVVIVAAEHAEEAAAVLSAQGETVSRIGVIRA
                     RAGDEHQTQIA"
     gene            4776..5405
                     /gene="purN"
                     /locus_tag="PKN55_10095"
     CDS             4776..5405
                     /gene="purN"
                     /locus_tag="PKN55_10095"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003784621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HNX71638.1"
                     /translation="MKNIVILISGRGSNMQAIVEAQLPGARIAAVIANRPDAAGLAWA
                     AERGIATAALDHKAFASREDFDAALAAQIDAFAPDLVVLAGFMRILTPGFTQHYAGRM
                     FNIHPSLLPAFPGLHTHQRALDAGCRLAGATVHFVTAELDHGPIVAQGAVAVADDDTA
                     DSLAAKVLRVEHQIYPQAVRWFVEGRLTLAGQRVRVDGGSADGALVCPR"
     gene            5380..>5791
                     /locus_tag="PKN55_10100"
     CDS             5380..>5791
                     /locus_tag="PKN55_10100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX71639.1"
                     /translation="MARWSARADWRLWLALLASLLAHLLWLTPDWAWLPAPARDEPPA
                     APLTARLQVLAAPTAAAKLAQAPTASPAAPLAKLATAPAPTRTKAQPTPSPADAVDAE
                     AADVAGQTAPEPAPVASAAPAEARFPARVSLRYEV"
BASE COUNT         1121 a         1867 c         1858 g          945 t
ORIGIN      
        1 gtgctgaccg ccggccccgc cgcgcaactg accctggcgc aactgcgcga cctgggcgtg
       61 cgccgcatca gcaccggcag cgccctcgcc cgcgccgcct tcactgccct ccaccacgcc
      121 gccgacgata tgctgaacaa gggaaacttt gcttatgcgc ggacggcgct gtcgggggag
      181 gtgttggacg gagtgatggg gaaagatgca tcagtattta tttaaaaatc aaatatatat
      241 caaaatactg agcaccgtaa tgatggccag ataaacaatg gcgggctgcg ccactaaagc
      301 caggggaatt ttgtcagcgg cacattaaaa ccgatccggg tcggccatga tctcgcctgc
      361 tcgggcgcgg atggccgcca gttcgtcggc cggcaggtcg gccagcagtt tgagcatcat
      421 cgccatgcgc gggttgccgg caaagtgggc ttttttgtcg tccagaaaac gccagtacag
      481 gctgttgaac gggcaggcgt cgtcaccggt gcgcgccttg acctgatagg cgcagccagc
      541 gcagtagttg ctcattttgt tgatataact gcctgaagac acatagggtt tgctggccag
      601 caggccgcca tcggcgtatt ggctcatgcc tcgggtattg ggcagctcca cccattcgat
      661 ggcgtcgata tacacgccca gataccaggc atccaccgcg tccgggtggc gctggctgag
      721 cagggcaaag ttgccaatca ccatcaagcg ctggatgtgg tgggcgtagg cgtggcgcag
      781 gctttgtgtc agcgccagat gcaggcagcg catgcgcgtg tggccggtcc agaacatctg
      841 cggcagcggg tggtgattgt ccagctggtt cagctggctg tattccggca tggccttcca
      901 gtacacgccg cgtacatact cgcgccagcc cagaatctgg cgcacaaagc cctcgatttg
      961 cgccaagctg atctgcgcgg gctgctgctg aaattgtgcc acggcggcgt tgatgacttc
     1021 tgcgggtgaa atcagcttga gattcagcgc aaacgacaag cgggaatgaa acaaaaacgc
     1081 ctgttcgctg tgcagggcgt cctggtattc gccaaagtgg gacagcaggt gttggcaaaa
     1141 atacgccagt tgcgccagcg cctcgtcgcg gttggccggc cagggaaacg ccgtggcgtc
     1201 gatctggcca aaaccgctga ccccggcggc ggtgatttcc gccagcagcg ccgacacatc
     1261 gcgggcaaag ctcagcgcgg gcggcaccgg cggcgtgcct ttgtattttt tgcggttgtc
     1321 gtggtcaaag ttccagcgtc cgcccagcgg ggccgcgcca tccagcaaga tcccgtgcgt
     1381 gcggcgcatg cgccggtaaa agcgctccat cagcaagggg gtgtcacccg gaaagcacgc
     1441 ggccaattca tcgcggcggg tgtaaaaatg cgcgctgtcg caagcctcgc ccggctgcgg
     1501 cagggattga ctgaacagca gcaattgctg atccagccgg tattcgtccg gctgctggta
     1561 ttcaaagcgc gccgcgccgt gctcggtgag tatctggcgc agcacctcgg gcagcggcag
     1621 ttggctggcg gcatcgccga tgcgcacata gtgcagccgg tggccagcag cgcgcagggt
     1681 gtcggcaaac tggcgcatgg ccagcaaaaa ccccagcagc ttttgccagt gatggcgcac
     1741 atagcgggct tcctggtgca gctcggccat caggtaaagc acgctgtcgt ccacctgggc
     1801 aaaccagggg tggcgaatgt tcagctggtc acccaggatc agccgcacgg tgcgaatggg
     1861 cggggccatc gggcgtgccc tcagcgtcgg cggcgataaa aacggctggc cagatagcgc
     1921 gcctgcgcca gcttgaccac caccaccaag gccgccgaca ccggcagcgc catcattacc
     1981 ccaacaaaac caaacagctg accaaacgcc agcagggcaa aaatcaccac caagggatgc
     2041 aggccaatgc gctcgcccac cagcttgggg gtgatgaaca tgctttccag cagctggccc
     2101 accacaaaca ccagccacac cagcagcagc ccgcccagcc cgccaaactg caacagcgcc
     2161 gccagcgtgg ccagggtcag gccgatggcg gcgcccacat aggggatgaa caccagcaag
     2221 ccggccacca cgccaatggc aaagccggat tccagcccca ccagccacag cccgccgccg
     2281 tacaccccgg ccatgatcag catcaccgcc agctggccgc gcaaaaactg gccgagcata
     2341 tcgtcgattt catcgccgag catgcccaag tcccgatacc agcggcgcgg aatcaagcgc
     2401 gccagcggcc gggtaaactg ctgccagtcg cgcaccagat aaaacaacac cagcggcagc
     2461 agcagcaaat tggacagata accaaacaag gccaggccct ggtcggtgat caagggcgcg
     2521 gcgcgcgcca gcatgccgcg cagcgcaccc agattgctgg tcagctcgcg cttgagcgtg
     2581 gcggcgtcaa aatgccaggt catgccccag gtgtcgcgca gccagggcga cagggtgttt
     2641 tccgcccagt ctactaagcc aggcaggcgc agcgacaagg cctggccctg gttgatcagc
     2701 atcggcacga tcaacaccaa taggcaggcc agcgccagca tcagcagcac aatcaccaac
     2761 agcgtgccca gcgtgcggcc cacgcgccga tgctccagcc aatccaccag cggcgccacg
     2821 atgtacgcca gcaccgcgcc agccacaaac ggcgacaaca tcggggtgag caaataaacc
     2881 agccagccaa aagccgtcag gccagccagc caaaaccagc catttctggc ggaatcgcga
     2941 gtgcgtttca tgatttgagg taaaataccg gagattgacc gcccaagatg gcggcaaaag
     3001 acagacagca ttcaatcccc acggcgatcc gtcaagcctg acctacccgg acaggcccat
     3061 ccacggcgcg ccggactgac gaaagtgagc gcattttgac ccaagcttcc ctctcctacc
     3121 gcgacgctgg cgtggacatc gacgccggcg accaactggt tgaaaacatc aagccgtttg
     3181 ccaagcggac gatgcgccct gaagtgctgg gcgatttggg cggctttggc gcgctggtgg
     3241 aaatctccaa gaaatacaaa gaaccggtgc tggtgtccgg caccgatggc gtgggcacca
     3301 agctcaagct ggccttcgac tggaaccgtc acgacaccgt cggcatcgac ctggtggcga
     3361 tgagcgtcaa cgacattctg gtgcaaggcg ccgagccgct gttcttcctg gattattttg
     3421 cctgcggcaa gctggacgtg ccgcaagcca ccgacgtgat caaaggcatc gccgccggct
     3481 gcgagcaagc cggctgcgcg ctgattggcg gcgaaaccgc cgagatgccg ggcatgtacc
     3541 cggtgggcga atacgacctg gctggctttg ccgttggcgt ggtggaaaaa acccaggtca
     3601 tcaccggccg cgacatcgtg ccgggcgacg tggtgctggg cctgaaatcc aacggcgtgc
     3661 attccaatgg ctacagcctg gtgcgcaaga ttctcgaccg cgcccagccc gagctggacg
     3721 ccgagttcga caatggcaaa accctgcgcg acgccatcat cgcccccacc cgcatttatg
     3781 tgaagccgct gctggcgctg atgcagcaac tgccggtcaa gggcatggcc cacatcaccg
     3841 gcggcggcat caccgaaaac accccgcgcg tgctgccggc caacaccgtg gcgcagatcg
     3901 acgccggcag ctgggagttg cccaagctgt tccagtggct gcaagcgcag ggcaatgtgg
     3961 acatgcagga aatgtaccgc acctttaact gcggcatcgg catggtggtg atcgtcgccg
     4021 ccgagcacgc cgaagaagct gctgcggtgc tgagcgccca gggggaaacc gtgtcgcgca
     4081 ttggcgtgat ccgcgcccgc gcaggcgacg agcatcagac gcagattgcc tgagtgaacg
     4141 ccgccctgcc gggcggcagg caaggcgtgg acgctgccgg gtgagcctgg cagcgtttgt
     4201 tgtttgcggg tggggctggc gtgagagatg ggatgcgctg acgcgcagtg tggctggggc
     4261 tggattttgg gcgggatttc accccgcacc cgaccccttt tctttgtctc gccaaagaaa
     4321 agggggaaaa gaaaggcgac ccgggtggcg cgcccttcgg gtgccctgtg cttctcgaaa
     4381 gtcagggccg tcccggaatg gcgcgtcggc tgcgccgccg cttagccggg ccggaaacct
     4441 ccctgacttt ctgcgatgct cggcgcgcca cacgggggtg aacggcggct aagcgcgacg
     4501 atgggggctt gggtttggtg tgtgtgggtg ttgactgacg ggggcatgag ggttgtgcta
     4561 tagcgtgtgc tcagtgaggt gcgctgagcg acgacaaggc cagtcgctta ttctctgtgc
     4621 acactgctgc tgtcccccat tctcgcgtcg cgggaaccca ggcaaagcgc gacgacaagc
     4681 tggtttgtct caccccctac ccgcccgcgc gccagcgcgg cccactcacc ccctcgccac
     4741 gctgaccgcc acaccgaata cactaaaaaa acatcatgaa aaacatcgtc attcttatct
     4801 ccggccgtgg ctcgaatatg caagccattg tcgaagctca actgcccggc gcgcgcattg
     4861 ctgcggtgat tgccaaccgg ccagacgccg ccggtttggc ctgggcggct gagcgtggca
     4921 ttgccaccgc cgcgctggat cataaagcct ttgccagccg cgaagatttc gacgccgcgc
     4981 tggcggcgca gatcgacgcc tttgcccccg acttggtggt gctggccggc tttatgcgca
     5041 ttctcacccc tggctttacc cagcattacg ccgggcggat gttcaatatc catccctcgc
     5101 tgctgccggc ttttccgggg ctgcacaccc atcagcgcgc gctggacgcc ggttgccggc
     5161 tggcgggggc gacggtgcat tttgtcaccg ccgagctgga ccacgggccg attgtcgccc
     5221 agggcgcggt ggcggtggcg gacgacgata ccgccgacag cctggcggcc aaggtgttgc
     5281 gcgtggagca tcagatttac ccgcaggcgg tgcgctggtt tgtggaaggc cggctgacgc
     5341 tggctggcca gcgcgtgcgg gtggacggcg gcagcgccga tggcgcgctg gtctgcccgc
     5401 gctgactggc ggctatggct ggcgctgctg gcctcgctgc tggcgcacct gctgtggctg
     5461 acgccggatt gggcgtggct gccggcgccc gcgcgtgacg agccgccagc cgcgcccttg
     5521 accgcgcgct tgcaggtgct ggccgccccc accgcggcgg ccaaactggc ccaggctccc
     5581 accgccagcc cggcggcgcc gttggcgaaa ctggcaacag cgcccgcgcc aacacggacc
     5641 aaggcgcagc ccaccccgtc gccagccgac gcggttgacg cggaggcggc tgacgtagcg
     5701 ggccagaccg cgcctgaacc cgcgcccgtt gcgtcagccg cgccggctga ggcgcgcttt
     5761 ccggcgcggg tcagcctgcg ctatgaagtg t
//
LOCUS       DAOPYX010000197         5776 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_17306_length_5776_cov_12.411641, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000197 DAOPYX010000000
VERSION     DAOPYX010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 5776)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5776)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5776
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17306_length_5776_cov_12.411641"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            60..590
                     /gene="tatB"
                     /locus_tag="PKN55_10105"
     CDS             60..590
                     /gene="tatB"
                     /locus_tag="PKN55_10105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014088623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HNX71640.1"
                     /translation="MFDISFGELAVIGVVALVVIGPEKLPKVARTVGALVGRAQRYVH
                     DVKADIQREVNLGELKQLHSEVHDAAQSLRNTVQSQVDEARQAVGEVQSQLNEVSQTA
                     THTLAEAQAQLDAVNQTVVEPLAPASSSASSAAAPSDTAPSAAHAALPAAEASPPSAV
                     AAAEAAAPLSSSSKPT"
     gene            587..1333
                     /gene="tatC"
                     /locus_tag="PKN55_10110"
     CDS             587..1333
                     /gene="tatC"
                     /locus_tag="PKN55_10110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012695687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HNX71641.1"
                     /translation="MTDSQPLLAHLIELRTRLVRALIGLGLMFLGLFHWSGDIYHLLA
                     KPLMQVLPAGAHMIATDVTTPFFVPMKVTMMVAFVLALPNTLYQIWAFVAPGLYAHEK
                     RLIVPLIISSVLLFITGMAFAYFLVFPVVFGFMSAVTPEGVSMMTDIDNYLSFVLGMF
                     IAFGITFEVPVVVVLLVRFGIVDIAKLKEARPYVIVGAFIVAAVVTPPDVLSQVMLAV
                     PLWLLFELGVVVAGWIGKSETTRANAKDAA"
     gene            1330..1680
                     /locus_tag="PKN55_10115"
     CDS             1330..1680
                     /locus_tag="PKN55_10115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008952128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2069 domain-containing protein"
                     /protein_id="HNX71642.1"
                     /translation="MKQTFHWLSVASLVALILLTLAWELWLAPLRPGGSLLALKAVVL
                     LLPLFGILRERVYTYQWSSMFILMFFAEGVTRAWGDKGLSQWLAGLETLLTVLFFTGV
                     LGYVRAVRQERQAA"
     gene            complement(1861..2607)
                     /gene="pxpA"
                     /locus_tag="PKN55_10120"
     CDS             complement(1861..2607)
                     /gene="pxpA"
                     /locus_tag="PKN55_10120"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011136720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HNX71643.1"
                     /translation="MHIDLNADLGEGCGDDAAILRYVSSANIACGWHAGDARTMRDTA
                     RAALAHGVALGAHPSFPDRAHFGRRAMQRAPAQVYQDMLDQIGALDEIVREEGGQLQH
                     VKPHGALYNQAAGDEALAEAIARAVHDYHPGLKLVGLAGGALVRQARLQGLNAVEEVF
                     ADRAYLADGSLAPRELPGAVIVNPVQAVAQTLRLVEQGQVTSQDGRTVQLRADSVCLH
                     GDSAHALDVARRLRARLEARGIQVCPQRAA"
     gene            complement(2917..3387)
                     /gene="rlmH"
                     /locus_tag="PKN55_10125"
     CDS             complement(2917..3387)
                     /gene="rlmH"
                     /locus_tag="PKN55_10125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HNX71644.1"
                     /translation="MKMTLLAVGSKMPRWVDDAFNEYARRLPREAALHLQEIKPEKRG
                     GGVTAEKGMAAECARIEAAIPPRSKLIVLDERGQDWTSVRLASEMKTWLESGDDICLV
                     IGGADGLSPAIKQRADVLLRLSALTLPHGMVRVLLAEQLYRAVSILNNHPYHRE"
     gene            complement(3465..3833)
                     /gene="rsfS"
                     /locus_tag="PKN55_10130"
     CDS             complement(3465..3833)
                     /gene="rsfS"
                     /locus_tag="PKN55_10130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011134073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HNX71645.1"
                     /translation="MDAQAIAALAVEALEDIKGKDIINLDTHKLTDLFQRMVVCTGDS
                     NRQVKALANSVHVKLKENGVDIVGTEGEASGEWVLVDAGDVVVHVMLPAVRDYYDIEA
                     LWGGEKPSFSPNAARPWSAL"
     gene            complement(4003..4644)
                     /gene="nadD"
                     /locus_tag="PKN55_10135"
     CDS             complement(4003..4644)
                     /gene="nadD"
                     /locus_tag="PKN55_10135"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011134074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HNX71646.1"
                     /translation="MSPRIGVFGGTFDPMHLAHRDMARALRDELALDKVHIVPAGQPY
                     HRAQAPQASAEQRLAMARLTLGDEAGLWVDAREVRRPRPAYTVETLTELRAEYGEAAE
                     LWLLIGGDSLAALPGWRHWQQIFTLANLAVWQRPDLPTPPLPPELAARWQARQVADFS
                     KRTQYGTIRALTLPAQAVSATAIRQALAAGQPCAGLLAPAVVDYINQQHLYGR"
     gene            complement(4641..5207)
                     /locus_tag="PKN55_10140"
     CDS             complement(4641..5207)
                     /locus_tag="PKN55_10140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008952052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HNX71647.1"
                     /translation="MSLLGDLLAHNREFVSNREYEKFETDKFPDKNLAVLACMDARLV
                     ELLPQAMGLKNGDAKLIKNAGALVTHQWGSVMRSLVVAVYELRAEEICVVAHHDCGMR
                     AIDPARILASAQERGVSADTIATLRGAGIDLDSWLKGFDNVHDSVRHTVATIRSHPLM
                     PKDIPVHGLVIHPATGQLDVVVDGYAPA"
     gene            complement(5498..>5776)
                     /locus_tag="PKN55_10145"
     CDS             complement(5498..>5776)
                     /locus_tag="PKN55_10145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX71648.1"
                     /translation="GALPGQALARIRVPTLLMHHQHDACTVCQPERVPALLPALSQAP
                     VKALWWIDGGGPPSGNICGPLHWHGFIGQDAELARRLAGWMRQPRATP"
BASE COUNT          962 a         1846 c         1921 g         1047 t
ORIGIN      
        1 ccgctgacag cggccgggtg atcgacgtca acaccgacca cagcaagaag taagccgccg
       61 tgttcgacat cagctttggc gaactggcgg tcatcggcgt ggtggcgctg gtggtgatcg
      121 gcccggaaaa actgcccaag gtggcgcgca ccgtcggcgc cttggtcggc cgcgcccagc
      181 gctatgtgca cgacgtcaag gcggatatcc agcgcgaggt gaatctgggc gagctcaagc
      241 aattgcacag cgaggtgcac gacgccgcgc aaagcctgcg caacaccgtg caaagccagg
      301 tggacgaagc ccgccaggcg gtgggtgagg tgcaaagcca gctgaacgaa gtcagccaga
      361 ccgccacgca cacgctggcc gaggcgcaag cacagctgga tgcagtcaat cagaccgtcg
      421 tcgagccgct ggcgccggcc agcagcagcg catccagcgc ggccgccccg tcggacactg
      481 cgcccagcgc agcccacgcc gccttgccgg cggccgaggc aagcccgccc agcgccgtgg
      541 ctgccgccga agccgccgcg cccctttctt cttccagcaa gccgacatga ccgactccca
      601 acccttgctt gcccacctga tcgagctgcg cacccgcctg gtgcgcgcgc tgattggcct
      661 ggggctgatg tttctgggcc tgtttcactg gtctggcgac atttaccact tgctggccaa
      721 gccgctgatg caggtgctgc cggctggcgc gcacatgatc gccaccgacg tcaccacgcc
      781 gttttttgtg ccgatgaaag tcaccatgat ggtggctttt gtgctggccc tgcccaatac
      841 gctgtaccag atctgggcgt ttgttgcgcc ggggctatat gcccatgaaa aacggctgat
      901 tgtgccgctg attatttcca gtgtgctgct gtttatcacc ggcatggctt ttgcgtattt
      961 tctggtgttt ccggtggtgt ttggctttat gagcgcggta accccggaag gcgtgtcgat
     1021 gatgaccgac atcgacaatt atttgtcgtt tgtgctgggc atgttcatcg cctttggcat
     1081 tacctttgaa gtgccggtgg tggtggtgct gttggtgcgc tttggcattg tcgatatcgc
     1141 caagctgaaa gaagcgcggc cgtatgtgat tgtcggcgcc tttattgtcg ccgccgtggt
     1201 caccccgccg gatgtgctgt cgcaagtgat gctggcggtg ccgctgtggc tgctgttcga
     1261 gctgggtgtg gtggtagccg gctggattgg caaatccgaa accacccgcg ccaacgccaa
     1321 ggacgccgca tgaagcagac ttttcattgg ctgagcgtcg ccagtctggt ggcgctgatt
     1381 ctgctgactc tggcctggga gctgtggctg gcgccgttgc gtccgggcgg cagcctgctg
     1441 gcgctcaagg ccgtggtgct gttgctgccg ctgtttggca tcctgcgcga gcgggtgtac
     1501 acctaccagt ggtcgagcat gttcatcctg atgttttttg ccgagggcgt cacccgcgcc
     1561 tggggcgaca aaggcctgtc gcaatggctt gccgggctgg aaaccctgct taccgtgctg
     1621 ttctttaccg gcgtgctggg ctatgtgcgc gccgtgcggc aagagcgcca ggcggcataa
     1681 gcggcgctga gcgcactcat gccagcgcag tgtgcggcga tgcccctgcc gccgtcacgc
     1741 cgctcacaac attccgcgcc agatgagtgc agatagtgcc gcgagtacgg caagattaca
     1801 caacaacgct ggatgggctt ggcgggcggt aagccgccac ggctcaccgc atcggccgca
     1861 tcaagcggct cgctgcggac acacctgaat cccccgcgct tccagccgcg cgcgcagccg
     1921 tcgggccaca tccagcgcat gcgcgctgtc accatgcaaa cagacgctgt cggcgcgcag
     1981 ctgcacggtg cggccatcct ggctggtgac ctggccttgc tcgaccagcc gcagggtttg
     2041 cgccaccgcc tggaccggat tgacgatcac cgcgcccggc agctcgcgcg gcgccaggct
     2101 gccgtcggcc agatacgcgc gatcggcaaa cacttcttcc acggcattca aaccctgcaa
     2161 acgcgcctga cgcaccagcg cgccgcccgc cagccccacc agcttcaggc cggggtggta
     2221 atcatgcacg gcgcgggcaa tcgcctcggc cagggcctca tcgcccgcgg cctggttata
     2281 cagcgcgcca tgcggcttga cgtgctgcaa ctggccgcct tcttcgcgga cgatttcatc
     2341 cagcgcgcca atctggtcga gcatgtcctg gtagacctgc gctggcgcgc gctgcatggc
     2401 acggcggcca aaatgcgcgc ggtcaggaaa actcggatgc gcccccagcg ccacgccatg
     2461 cgccaaggcg gcgcgcgccg tgtcgcgcat ggtgcgcgcg tcgcccgcat gccagccaca
     2521 ggcaatattg gcagaactga catagcgcaa aatcgcggcg tcgtcgccgc agccttcacc
     2581 gagatcggca ttgaggtcga tgtgcatcac aggctccgca ggtaaaacaa aatggtagaa
     2641 acgcccggcg caatgcggcg caataagagc cgctaacaaa actcagcgaa gcgctcctgg
     2701 ctaggcgcgc ggccgcagac agtacaagga gtacggcaag gtcgcgcaac gacgccagga
     2761 gggttttgtt agcggctcta agccagattg cagggtttca ctctacctat ttcatcgacg
     2821 caggaatacc ctgctaaagc acaagtttat tacatttact ttcaccgcca cgatattgcc
     2881 gcgagatggc caatatgtgc agaacacgcc aaattttcac tcccgatgat acggatgatt
     2941 gttcaggatc gacaccgcgc ggtaaagctg ttcggccagc agcacgcgca ccatgccgtg
     3001 cggcagggtc agtgcggaga ggcgcagcag cacgtcggcg cgttgtttga tggcggggga
     3061 gaggccgtcg gcgccgccga tcaccaggca gatgtcgtcg ccgctttcca gccaggtttt
     3121 catttcgctg gccagacgca cgctggtcca gtcctggccg cgttcgtcca gcacgatcag
     3181 cttgctgcgc ggcgggatgg cggcctcgat gcgggcgcat tcggcggcca tgcctttttc
     3241 ggcggtgacg ccgccgccgc gtttttcggg tttgatttcc tgcaaatgca gggcggcctc
     3301 acgcggcaga cgacgggcgt attcattgaa cgcgtcgtcc acccagcgcg gcattttgct
     3361 gccgacggcc agcagggtca tcttcatggg aacaccggca ggaagagggg agcgccgcgc
     3421 gcgccaggca acgttggcgc gggactagcg gcgcggagcg atgcttacag cgcgctccac
     3481 gggcgggcgg cgttcgggct gaacgacggc ttttcgccgc cccacagcgc ttcgatgtcg
     3541 tagtagtcgc gcacggccgg cagcatcaca tgcaccacca cgtcgccggc atccaccagc
     3601 acccattcgc ccgaggcttc gccttcggtg ccgacgatgt ccacgccgtt ttccttgagc
     3661 ttgacgtgca cgctgttggc cagcgccttg acctggcggt tggagtcgcc ggtgcacacc
     3721 accatgcgct ggaacaggtc ggtcagcttg tgggtgtcga ggttgatgat gtccttgccc
     3781 ttgatgtctt ccagggcttc taccgccagg gcggcaatcg cttgtgcgtc catgatgtcc
     3841 ttaatgagtg tgaggctggg ccgccgggct gctcagcgcg gctcacaaaa ctctcctggc
     3901 gtgcttgcct gacggcactg tcgcctagcc aggatcgctg cgctgagttt tgttcgccgc
     3961 tttgagtgtg gctcccacgg tccttgtgct gtggcgagcc gctcagcggc cgtacaaatg
     4021 ctgttgatta atatagtcca ccacggcggg ggccaatagc ccggcgcagg gctggccggc
     4081 ggccagtgct tggcggatgg ccgtggcgga aaccgcctgg gccggcaaag tcagggcgcg
     4141 gattgtaccg tactgcgttc gttttgaaaa atcagccact tgccgcgcct gccagcgcgc
     4201 ggccagttcg ggcggcaagg gtggggtggg caggtcgggg cgctgccaca ccgccagatt
     4261 ggccagggta aaaatctgct gccagtggcg ccagccgggc agcgccgcca gcgagtcgcc
     4321 gccgatcagc agccacaatt cggcggcctc gccgtattcg gcgcgcagtt cggtgagggt
     4381 ttctaccgtg taggccgggc gtgggcggcg cacttcgcgc gcgtccaccc acaggccggc
     4441 ttcgtcgccg agggtgagcc gggccatggc cagccgctgt tcggcgctgg cctgcggcgc
     4501 ttgcgcgcgg tgatagggct ggccggccgg cacgatgtgc actttgtcca gcgccagctc
     4561 gtcgcgcaag gcgcgcgcca tgtcccgatg cgccaggtgc atcgggtcga aggtgccgcc
     4621 aaatacgccg atgcgtgggc tcatgccggc gcgtagccgt ccaccaccac gtccagctgg
     4681 ccggtggccg ggtgaatcac caggccgtgc accggaatat ccttgggcat cagcggatgg
     4741 ctgcggatgg tggccacggt gtggcgcacg ctgtcgtgga cattgtcaaa acccttcagc
     4801 cagctgtcca ggtcgatgcc ggcgccgcgc agggtggcga tggtgtcggc ggacacgccg
     4861 cgttcttgcg cgctggccag gatgcgcgcc gggtcgatgg cgcgcatgcc gcagtcgtgg
     4921 tgggccacca cgcagatttc ttcggcgcgc agctcgtaca ccgccaccac caggctgcgc
     4981 atcaccgaac cccactggtg ggtgaccagg gcgccggcgt ttttgatcag cttggcgtcg
     5041 ccgtttttca ggcccatggc ctgcggcagc agctcgacca gccgggcgtc catgcacgcc
     5101 agcacggcca ggtttttgtc ggggaattta tcggtttcga atttttcgta ttcacggtta
     5161 ctgacaaact cgcggttatg cgcgagcaga tcgcccaaca ggctcattga atggtctccg
     5221 atgaaaggcg cgccgtcggc acagccggtg ggctgggacg ggcgcggggc aggacggaag
     5281 gcgcgggtca tggggggcgc gcaacgccgg gcgatgctat cacggccagc gcaaaaccgg
     5341 ataagagtcg ctcacaaaac cccgtgcagc gcgcttggcg aggcgcacga ccgcagacag
     5401 tgcgggggta cggcaagctc gggcaaccac gccaggaagg tgctgtgagc agcgccaggc
     5461 aaacctccgc ctgatgcgcg cagagaatca atccccctca gggcgtggcg cgcggctggc
     5521 gcatccagcc ggccagccgg cgcgccagct cggcatcctg gccaataaag ccatgccagt
     5581 gcagcgggcc gcaaatattg ccggagggcg ggccgccgcc gtcgatccac cacagcgcct
     5641 tgactggcgc ctggctgagc gccggcaata gcgcaggcac tcgctcgggc tggcagacgg
     5701 tgcaggcatc gtgctggtgg tgcatcagca aggtgggcac gcggatgcgc gccagcgcct
     5761 ggccgggcag cgcgcc
//
LOCUS       DAOPYX010000198         5725 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_17537_length_5725_cov_14.064727, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000198 DAOPYX010000000
VERSION     DAOPYX010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 5725)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5725)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5725
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17537_length_5725_cov_14.064727"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..131
                     /locus_tag="PKN55_10150"
     CDS             <1..131
                     /locus_tag="PKN55_10150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012695518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HNX71649.1"
                     /translation="GEINKIYAKWFTSPIPPKNVNLNLPMNAQLQEAIKNPNDKGI"
     gene            215..961
                     /locus_tag="PKN55_10155"
     CDS             215..961
                     /locus_tag="PKN55_10155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012695519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HNX71650.1"
                     /translation="MSQYQWNWSVVLEQVSGTEEFYYEWLAAGLGWTVATALSAWVLA
                     LVVGSLIGILRTVPSKPVAALATAYVEVFRNIPLIVQMFLWYFVLPELVPHNLGIWMK
                     QSMPLPEFSTAVIALGFFTSARVAEQVRTGILALPRGQKNAGLAMGFTLGQTYRYVLL
                     PMAYRIVIPPLTSEFMNIFKNSAVALAIGLTELTFQMRQITGEYAPANPIEVMTVVSA
                     LYLLVAFSVNRVMALIERKTRVPGYISGGH"
     gene            961..1620
                     /locus_tag="PKN55_10160"
     CDS             961..1620
                     /locus_tag="PKN55_10160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012695520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HNX71651.1"
                     /translation="MNLDFSTLAGAQDFLWQGFKYSLTLTVSSMVFGIFFGTLLAMAR
                     MSSIKPLSLAAAGYVNLFRSVPLVLVIFWFYILVPLLTGVDVGAERSAFLTFAIFEAA
                     YYCEIIRAGIQSIPRGQIAAGQAMGFSYGQNMRYVILPQAFRNVVPLLLTQTIVLFQD
                     TSLVYVVGATDLLGAADKVAHRDNTLVEMYMLVALIYFSISFALSQAVKRLHARIAIV
                     R"
     gene            1640..2374
                     /locus_tag="PKN55_10165"
     CDS             1640..2374
                     /locus_tag="PKN55_10165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011133672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HNX71652.1"
                     /translation="MIQINNVSKWYGEFQVLTDCTTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNALEPFQKGEILVEGVSVGDPKTNLPKLRSRVGMVFQHFELFPHLSITDNLIIAQ
                     MKVLGRGRDEAMDKGLKLLDRVGLKAHAHKFPGQLSGGQQQRVAIARALAMDPIVMLF
                     DEPTSALDPEMVNEVLDVMVELAQEGMTMMCVTHEMGFARKVASRVIFMDRGQIVEDC
                     SKDDFFGHLDARSERARQFLSKILQH"
     gene            2456..4414
                     /locus_tag="PKN55_10170"
     CDS             2456..4414
                     /locus_tag="PKN55_10170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008953810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNX71653.1"
                     /translation="MTDPAASPYRPAIAPPSPLWRRPTYGAPWVGPFSPPPRPRVVRS
                     LLLPVLMLAMMALIGYAGFRISEHTGIAALRESGARQMALHARGVESEIGKFTLLPRL
                     LEMEPKVMNLLETPSPRLRREVNLYLDGFNDRSGSRVVYVIDPSGRVQASSNWDDFDS
                     YVDEDLSFRAYFQDAIVGKPGRFYGIGSTIGESGYYLAHSLKNHGHIVGVGVVKVRLE
                     SVEQRWRDAGLQAFVSDENQIIILASDPAHKLRAVTPLSPQKREKLALSLQYHWWALT
                     DLKTLRREPLGPGEELITFPSERRAPGGVRYLALSQPLADTPWRFTLLLPLEEIRRNA
                     TSHGALAAVLFAFVSFLLIAWNERRKVIATRLAAREALQRANNALERRIAERTADLRA
                     SNARLKAEIREREHTELTLRRTQDDLIQAGKLAVIGQMATSIAHELNQPLAALRTLSG
                     NTVRFLERGKLDVASANLGTINQLVDRMGKITASLRSFARRSDPSDSRASLTQAVDAA
                     LFLVEPRKGKGPLTLIRDFADCQLAIDQTRLEQILVNLFSNALDAMQGQQERVLALRG
                     EAGDSHYEFHVRDNGRGLPPDTMPRLFEPFFTTKPAAQGGLGLGLTISASLAADAGGS
                     LTARQPDGGGAEFVLALPLLPTETPPHD"
     gene            4407..>5725
                     /locus_tag="PKN55_10175"
     CDS             4407..>5725
                     /locus_tag="PKN55_10175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008953811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNX71654.1"
                     /translation="MTDETLSVLIVEDDPHVMLGCQQALALEDIPSEGVNSAEAALAR
                     LDDDFAGVVVSDIRLPGMDGLTLQKQLAARDRKLPVILITGHGDISMAVNAIQQGAYD
                     FIEKPFSPERLVEVCRRALEQRRLTREVRSLRRQLAGRQALEERIIGHSPAIRTLRTL
                     IANVADTAANVLIEGETGTGKELVARCLHDFSSRRDKEFVAINCGGLPENLFESEIFG
                     HEAHAFTGAGKRRIGKIEYAHGGSLFLDEVESMPINMQIKLLRVLQERQLERLGSNSA
                     VAVDCRVIAATKSDLEAMGRDGRFRSDFYYRLNVVTLELPPLRERREDIPLLFEHFLQ
                     QAALRFDRESPELDPATLAALMRHDWPGNVRELRNVAERYALGLPVFRKTGAAPDSHT
                     PRLAEAVEAFEKSLIRDTLERHGGNLSQASVALGIAKTTLFDKVRKYG"
BASE COUNT         1044 a         1861 c         1779 g         1041 t
ORIGIN      
        1 cgggcgagat caacaagatc tacgccaagt ggttcaccag cccgattccg ccgaagaacg
       61 tcaacctgaa cctgccgatg aacgcgcagc tgcaggaagc catcaagaac ccgaacgaca
      121 agggtatctg attgacccgg ccccattggg ccggataggc gaatggggcg gcccgcctgg
      181 tccggcggcc gccccggcaa taaaggacaa ccccatgagt caatatcaat ggaactggtc
      241 tgtggtgctg gagcaagtgt ccggcactga agagttctat tacgagtggc tggcggccgg
      301 cctgggctgg acggtggcca cggccttgtc ggcctgggtg ctggcgctgg tcgtcggttc
      361 gctgatcggc attctgcgca ccgtgccctc caaaccggtg gcggcgctgg ccaccgccta
      421 tgttgaagtg tttcgcaata tcccgctgat tgtgcagatg ttcctgtggt atttcgtgct
      481 gcccgagctg gtgccgcaca atctgggcat ctggatgaag cagtccatgc cgctgccgga
      541 attctccacg gcggtgattg ccctgggctt tttcacctcg gcgcgggtgg ccgagcaagt
      601 gcgcaccggc attctggcgc tgccgcgcgg ccagaaaaac gccggcctgg ccatgggctt
      661 caccctgggc cagacctacc gctatgtgct gctgccgatg gcgtatcgga ttgtgattcc
      721 gccgctgacc agcgaattca tgaacatctt caaaaactcc gccgtggcgc tggccatcgg
      781 cctgaccgag ctgaccttcc agatgcgcca gatcacgggc gaatatgcgc cggccaaccc
      841 gattgaggtg atgaccgtgg tcagtgcgct gtatctgttg gtggcgtttt ccgtcaaccg
      901 ggtgatggcg ctgatcgaac gcaagacccg cgtgccgggc tatatctccg gaggccattg
      961 atgaacctcg atttctcgac gctggccggc gcccaggatt ttctgtggca aggcttcaag
     1021 tattcgctga cgctgacggt cagttcgatg gtgttcggca ttttctttgg cacgctgctg
     1081 gccatggcgc gcatgtccag catcaagccg ctgtcgctgg ctgccgccgg ttatgtgaac
     1141 ctgttccgct cggtgccgct ggtgctggtg attttctggt tttacattct ggtgccgctg
     1201 ctgaccgggg tggacgtggg cgctgaacgc tcggcttttt tgaccttcgc catctttgag
     1261 gcagcgtatt actgcgaaat catccgcgcc ggcattcaat ccatcccgcg tggacagatt
     1321 gccgccggcc aggccatggg cttcagctac ggccagaaca tgcgctatgt gatcctgccg
     1381 caggccttcc gcaatgtggt gccgctgctg ctgacccaga ccatcgtgct gttccaggat
     1441 acttcgctgg tgtatgtggt gggcgccacc gacctgctgg gcgccgctga caaagtggct
     1501 caccgcgaca acaccctggt ggaaatgtac atgctggtgg cgctgattta cttcagcatc
     1561 agctttgccc tgtcgcaggc cgtcaagcgt ctgcacgccc gcatcgccat cgtgcgctaa
     1621 atttgtaagg aacagactga tgatccagat caacaatgtg tccaagtggt acggtgagtt
     1681 tcaggtgctg accgactgca ccaccgaggt gaaaaaaggc gaggtggtgg tggtgtgcgg
     1741 gccgtccggc tcgggcaaat ccaccctgat caaaaccgtg aacgcgctgg agccgttcca
     1801 gaagggcgaa atcctggtgg aaggcgtgtc ggtgggcgac ccgaaaacca atctgcccaa
     1861 gctgcgcagc cgggtgggca tggtgttcca gcactttgag ctgtttccgc acctgtcgat
     1921 caccgacaac ctgatcatcg cccagatgaa ggtgctcggc cgtggccgcg acgaggccat
     1981 ggacaagggc ctgaagctgc tggaccgcgt gggcctgaag gcgcacgcgc acaaattccc
     2041 cggccagctc tccggcggcc agcagcagcg cgtggcgatt gcccgcgccc tggccatgga
     2101 cccgatcgtg atgctgtttg acgagccgac cagcgcgctg gacccggaaa tggtcaacga
     2161 agtgctggat gtgatggtcg agctggcgca agaaggcatg accatgatgt gcgtcaccca
     2221 cgaaatgggc tttgcccgca aggtggccag ccgggtgatc tttatggatc gcggccagat
     2281 cgtcgaagat tgcagcaagg atgatttctt tggccatctg gacgcccgtt ccgagcgcgc
     2341 gcgccagttc ttgtccaaaa tcctgcaaca ctaagcgcca tgcacgccgg gtgcgtcaac
     2401 gcgcccggcc ggcgctgtct tgtatcatgc gcatgaccct ctgcgcccac ctcttatgac
     2461 cgaccccgcc gcctctcctt accgtccggc cattgccccg cccagcccgc tgtggcggcg
     2521 gccgacgtat ggtgcgccct gggtggggcc gttttctccg ccaccgcgtc cgcgcgtggt
     2581 gcgttcactg ctgttgcccg tgctgatgtt ggccatgatg gcgctgattg gctacgctgg
     2641 ctttcgcatc agcgagcaca ccggcatcgc cgcgctgcgc gaaagcggcg cgcggcaaat
     2701 ggccttgcat gcacgtgggg tagaaagcga aattggcaaa ttcaccttgc tgccgcgcct
     2761 gctggaaatg gagcccaagg tgatgaatct gctggaaacc cccagccctc gcctgcgccg
     2821 cgaagtgaat ctttatctgg atggcttcaa tgaccgcagc ggcagccgcg tggtgtatgt
     2881 catcgacccc agcggccggg tgcaggcttc cagcaactgg gacgattttg acagctacgt
     2941 tgatgaagac ctgtcgtttc gcgcctattt tcaggatgcg attgtcggca aacccggacg
     3001 cttttacggc attggcagca ccattggcga atcgggctac tatctggcgc acagcctgaa
     3061 aaaccacggc catattgtcg gcgtcggcgt ggtcaaggtg cggctggaat ccgtcgaaca
     3121 gcgctggcgc gacgccggct tgcaggcttt tgtcagcgac gaaaaccaga tcatcatcct
     3181 ggccagcgac ccggcgcaca aactgcgcgc cgtcaccccg ctcagcccac aaaagcgcga
     3241 aaaactggcg ctcagcctgc aataccactg gtgggcgctg accgacctga aaaccctgcg
     3301 ccgcgagccc ctgggccccg gcgaagagct gatcaccttt ccgtccgagc ggcgcgcccc
     3361 cggcggcgtg cgctatctgg cgctcagcca gccgctggcc gacaccccct ggcgctttac
     3421 cctgctgttg ccgctggaag aaatccgccg caacgccacc agccatggcg cgctggcggc
     3481 ggtgctgttt gcctttgtca gctttttgct gatcgcctgg aacgagcggc gcaaagtcat
     3541 cgccacccgg ctggcggcgc gcgaggcgct gcaacgcgcc aataatgcgc tggagcggcg
     3601 cattgccgaa cgcaccgccg acctgcgcgc cagcaacgcc cgcctgaaag ccgaaatccg
     3661 cgagcgcgaa cacaccgaac tcaccctgcg ccgcacccag gacgacctga tccaggccgg
     3721 caagctggcg gtgattggcc agatggccac cagcatcgcc cacgagctga accaaccctt
     3781 ggccgcgctg cgcaccttgt ccggcaacac cgtgcgcttt ctcgagcgcg gcaagctgga
     3841 tgtggccagc gccaatctgg gcaccatcaa tcaactggtg gaccgcatgg gcaaaatcac
     3901 cgccagcctg cgctcgtttg cccgccgctc cgacccctcc gacagccgcg ccagcctcac
     3961 ccaggcggtg gacgccgcgc tgtttctggt ggagccgcgc aagggcaagg gcccgctgac
     4021 gctgatccgc gactttgccg actgccagct ggccatcgac caaacccggc tggagcagat
     4081 tctggtgaat ctgttctcca atgcgctgga cgccatgcag ggccagcaag agcgcgtgct
     4141 cgccctgcgc ggcgaagccg gcgacagcca ttatgaattt catgtgcgcg acaacggccg
     4201 tggcctgccg cccgacacca tgccgcgcct gttcgaaccg tttttcacca ccaaacccgc
     4261 cgcccagggc ggcctggggc tgggcctgac catttccgcc agcctggccg ccgacgccgg
     4321 cggctcgctg accgcccgcc agccagacgg cggcggcgcc gaatttgtgc tggccctgcc
     4381 cctgttgccc acggagaccc cgccccatga ctgacgaaac gctaagcgtg ctgattgtcg
     4441 aagacgaccc tcatgtgatg ctgggctgcc agcaggcgct ggcgctggaa gacatcccca
     4501 gcgaaggggt caacagcgcc gaggccgcgc tggcgcggct ggacgacgac tttgccggcg
     4561 tggtggtcag cgacatccgc ctgcccggca tggacggcct gaccctgcaa aagcaactgg
     4621 ccgcgcgcga ccgcaagctg ccggtgatcc tgatcaccgg ccatggcgat atctcgatgg
     4681 cggtcaacgc catccagcaa ggcgcctatg actttatcga aaaacccttc tcgcccgagc
     4741 ggctggtgga agtgtgccgg cgcgcactgg aacagcgccg gctcacccgc gaagtgcgca
     4801 gcctgcgccg ccagctggcc ggccgccagg cgctggaaga acgcatcatc ggccactccc
     4861 cggccatccg caccctgcgc acgctgattg ccaatgtggc tgacaccgct gccaatgtgc
     4921 tgatcgaggg tgaaaccggc accggcaaag agctggtggc gcgctgcctg catgattttt
     4981 ccagccggcg cgacaaagaa tttgtcgcca tcaactgcgg cggcctgcca gaaaatttat
     5041 tcgagagcga aatcttcggc cacgaagcgc acgcctttac cggcgcgggc aagcggcgca
     5101 tcggcaaaat cgaatacgcc catggcggct cgctgtttct ggatgaagtg gaaagcatgc
     5161 cgatcaatat gcagatcaag ctgctgcgcg tgctgcaaga gcgccagctg gagcggctgg
     5221 gctcgaacag cgcggtggcg gtggactgcc gggtgatcgc cgccaccaaa tccgacctgg
     5281 aagccatggg ccgcgacggc cgctttcgca gcgattttta ctaccggctg aatgtggtga
     5341 cgctggaact gccgccgctg cgcgaacggc gcgaagacat cccgctgctg ttcgaacact
     5401 ttttgcaaca agccgcattg cgctttgacc gggaaagccc ggaactggac ccggccaccc
     5461 tggccgcgct gatgcgccac gactggccgg gcaatgtgcg cgaactgcgc aatgtggccg
     5521 aacgctacgc cctgggcctg ccggtgttcc gcaaaaccgg cgccgccccg gacagccaca
     5581 ccccgcgcct ggccgaagcg gtggaagcct tcgaaaaaag cctgatccgc gacacgctgg
     5641 aacgccacgg cggcaacctc agccaggcca gcgtggcgct gggcatcgcc aaaaccacgc
     5701 tgtttgacaa ggtgcgcaag tatgg
//
LOCUS       DAOPYX010000199         5717 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_17566_length_5717_cov_11.262451, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000199 DAOPYX010000000
VERSION     DAOPYX010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 5717)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5717)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5717
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17566_length_5717_cov_11.262451"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1958
                     /locus_tag="PKN55_10180"
     CDS             <1..1958
                     /locus_tag="PKN55_10180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011133989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNX71655.1"
                     /translation="LTLFGMVLAIGIVVDDAIVVLENVERIMEEDKLSPVDATLKAMQ
                     EVTGPVIAIVLVLCAVFIPVAFLGGLAGQMYKQFAITIAMSVVISGIVALTLTPALCA
                     VLLKPGHQAAHKFFDVFNNWFARMTGRYTRGVAFFVRRSALGLLVCGGIVAVTLGLFR
                     VVPGGLVPDEDQGYVIAVTLLPDGASLSRTQHATQQVDKLAMAHPAVTDVLTFTGFDL
                     LTGSFKTNSGASFLTLKDWKERRAESDQAQSIVGYMFGAGMGVKEAMVLAFNPPPISG
                     MSSTGGFEVYVQNRGNGGSKDLAAATQKLIEAAHKRPELGQLSTTFSADVPQLFIELD
                     REKAKTYGVPINDVFDTMQSTFGELYVNDFNKLGRTFRVQLQSEADFRSRPEDIRNIH
                     VRSQSGEMIPLTALVTVKQVTGPEIVERYNIFPSAKIVGGPAPGYSSGQAIAAMEELA
                     AQVLSDDYSLAWTGSAFQEKQTGGASTLVFVFALVMVFLILAAQYERWNLPIAVLLAV
                     PFAVFGAISANWLRGMANDVYFQIALVTLIGLAAKNAILIVEFAMLKLEEGLSLADAA
                     IAAARLRFRPIIMTSLAFVLGCVPLAISTGAGASSRHSIGTGVIGGMLAATFIATFFI
                     PMLFMLIMRLGRRPAPVAVSQHDGKDA"
     gene            1958..3376
                     /locus_tag="PKN55_10185"
     CDS             1958..3376
                     /locus_tag="PKN55_10185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011133988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HNX71656.1"
                     /translation="MRRLILLALTSGLLSACAIGPDYQRDTPPLPDAWRLDAEAHKPA
                     LDARWWTRFDDPVLDALIAAAEQHNQDLRLAVARVDEARALAGIAQADLLPSLNASAQ
                     GSRARGSTVGLQPVPGRPIVEDKQAAATLSFELDLWGKLRRANEAAQASLLSSQANRD
                     AVRLTLLAQVAQGYFSLRALDEQFSLAESTLATRQQALLLQEKRFKGGITSELDYRQA
                     QAEAAVAAAAVPALAQQAQQAESALAVLVGRSPRDIVAGGLPRGKAIGALTAPPAVPA
                     GLPSSLLTRRPDLQAAEQNLVAANARIGVARAAFLPSISLTGLLGSESVALNNLFNGP
                     AATWSFAANLAMPLFDFGKTAAGVDAASARQRQALAQYQLAIQTAFKEAQDALVAHRK
                     TREAAEARATQVGALKRSLRLARLRYDNGYSSYLEVLDAERSLFQAELDAIDAQRQRL
                     SATVELYKALGGGWQADKADKS"
     gene            3709..4194
                     /gene="bfr"
                     /locus_tag="PKN55_10190"
     CDS             3709..4194
                     /gene="bfr"
                     /locus_tag="PKN55_10190"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HNX71657.1"
                     /translation="MKGDNTIIDILNELLAGELTAVDQYLIHGEMYADMGLHALAEKT
                     IHESEHERQHARALIQRILFLEGTPNLAKREPLHVGQTVPEMLQADLDVEYKVDAALK
                     QAIAACEAAQDYVSRDMLQVQLEDTEVDHAYFLEKQLRLIKLVTLPNYLQSQMGSGSP
                     T"
     gene            4210..4683
                     /gene="bfr"
                     /locus_tag="PKN55_10195"
     CDS             4210..4683
                     /gene="bfr"
                     /locus_tag="PKN55_10195"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HNX71658.1"
                     /translation="MQGDKQVIDTLNKVLRNELTAINQYFLHARMFRNWGLEKLNDYQ
                     YKQSIRVMKEADELIERILFLEGLPNLQDLGKLLIGEHVEECLQGDLTYERDIQRPLL
                     VQAIALCETAGDYVSRELLEDLLEHCEEAIDWLETQHSLIANVTLPNYLQAHMDD"
     gene            5019..5702
                     /locus_tag="PKN55_10200"
     CDS             5019..5702
                     /locus_tag="PKN55_10200"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003797124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HNX71659.1"
                     /translation="MFSSLRPLALAAALLPSLAFAQFSLPPLPYAADALEPVIDKTTM
                     EIHHDRHHAAYVNNLNAQIKSYPELDHLSLDALQGKISQYNAAVRNNGGGHYNHDLFW
                     KVMAEPGLGGEPSAALKQAIDAQFGNLDELKKRFDQAAVSRFGSGWAWLVVTADKKLA
                     ITSTANQDNPLMDLPEIAQRGAPILALDVWEHAYYLKYQNKRADYVTQWWKVVNWNEV
                     SRRYAAALR"
BASE COUNT         1054 a         1879 c         1769 g         1015 t
ORIGIN      
        1 cgctgacctt gttcggcatg gtgctggcca tcggcattgt ggtggatgac gccatcgtgg
       61 tgctggaaaa cgtcgaacgc atcatggagg aagacaagct ctcgccagtg gacgccacgc
      121 tgaaagccat gcaggaagtc accggcccgg tgatcgccat tgtgctggtg ctgtgcgcgg
      181 tgtttattcc ggtggcgttt ctgggcggcc tggccgggca gatgtacaag cagttcgcca
      241 tcaccatcgc catgtcggtg gtgatttccg gcattgtcgc gctgacgctg accccggcgc
      301 tgtgcgcggt gttgctcaag cctggccatc aggcggcgca caagttcttt gacgtgttca
      361 ataactggtt tgcccgcatg accggccgct acacccgtgg cgtggcgttt tttgtgcgcc
      421 gcagcgcgct gggcctgttg gtgtgcggcg gcattgtcgc ggtcaccctc ggtttgttcc
      481 gcgtggtgcc gggcggcctg gtgccggacg aagaccaggg ctatgtgatt gccgtgaccc
      541 tgctgcccga cggcgcgtcg ctgtcgcgca cccagcacgc cacccagcag gtggataaat
      601 tggccatggc ccacccggca gtgaccgatg tgctgacctt caccggcttt gacctgctca
      661 ccggctcgtt caagaccaac tcgggcgctt cgttcctgac gctgaaagac tggaaagaac
      721 gccgcgccga aagcgaccag gcgcagtcga ttgtcggcta tatgtttggc gccggcatgg
      781 gcgtgaaaga agccatggtg ctggccttca acccgccgcc gatttccggc atgtccagca
      841 ccggcggctt tgaggtgtat gtgcaaaacc gtggcaatgg cggctcgaaa gacctggccg
      901 ccgccacgca aaagctgatc gaagccgcgc acaagcgccc cgagcttggc cagttgtcca
      961 ccaccttcag cgccgatgtg ccgcagttgt tcatcgagct ggatcgggaa aaagccaaga
     1021 cctacggcgt gccgatcaac gatgtgttcg acaccatgca aagcaccttt ggcgagctgt
     1081 acgtcaacga cttcaacaag ctgggccgca ccttccgcgt gcagctgcaa tcggaagccg
     1141 acttccgctc gcgcccggag gatatccgca atatccacgt gcgctcgcaa agcggcgaga
     1201 tgatcccgct gaccgcgctg gtgacggtca agcaggtgac cggcccggaa atcgtcgaac
     1261 gctacaatat cttcccgtcg gccaagattg tcggcggccc ggcgccgggc tacagctcgg
     1321 gccaggcgat tgccgccatg gaagaactgg ccgcgcaagt gttgtccgac gattacagcc
     1381 tggcctggac cggctcggcc ttccaggaaa aacaaaccgg cggcgcctcc accctggtgt
     1441 ttgtgtttgc cctggtgatg gtgtttttga ttctggccgc gcagtacgag cgctggaacc
     1501 tgccgattgc cgtgctgctg gcggtgccgt ttgccgtgtt tggcgccatc agcgccaact
     1561 ggctgcgcgg catggccaat gatgtgtact tccagattgc cctggtgacg ctgattggcc
     1621 tggcggcgaa aaacgccatc ctgattgtgg aattcgccat gctcaagctg gaagaaggcc
     1681 tgtcgctggc cgacgcggcc attgccgccg cccgcctgcg tttccggccg atcatcatga
     1741 cctcgctggc ctttgtgctg ggctgcgtgc cgctggccat cagcaccggc gctggcgcgt
     1801 cctcgcgcca ttcgattggc accggggtga ttggcggcat gctggcggcc acgtttattg
     1861 ccacgttttt cattccgatg ttgttcatgc tgatcatgcg cctgggccgt cgccccgcgc
     1921 cggtcgcggt cagccagcat gatggaaagg acgcctgatg cgccgactga ttttgcttgc
     1981 actcacctct ggcctgttgt cggcctgcgc catcggcccc gactaccagc gcgacacccc
     2041 gccgctgccc gacgcctggc ggctggacgc cgaggcgcac aagccggcgc tggacgcgcg
     2101 ctggtggacg cgctttgacg acccggtgct ggacgcgctg atcgccgccg ccgaacaaca
     2161 caaccaggac ttgcgcctgg cggtggcgcg ggtggacgag gcgcgtgcgc tggccggcat
     2221 cgcccaggcc gacctgctgc ccagcctgaa cgccagcgcc cagggcagcc gcgcccgcgg
     2281 cagcacggtg ggcctgcaac ctgtgcccgg ccggccgatt gtggaagaca aacaagccgc
     2341 cgccacgctg agctttgagc tggacctctg gggcaagctg cgccgcgcca acgaagccgc
     2401 gcaagccagc ctgttgtcca gccaggccaa ccgcgacgcc gtgcggctga ccctgctggc
     2461 gcaggtggcg cagggctatt tcagcctgcg cgcgctggat gagcaattca gcctggcgga
     2521 aagcacgctg gccacccgcc agcaggcgct gctcttgcag gaaaaacgct tcaagggcgg
     2581 catcacctcc gagctggact accgccaggc gcaggccgaa gcggcagtgg ccgccgccgc
     2641 cgtgccggcg ctggcgcagc aggcgcaaca agccgaaagc gcgctggcgg tgctggtggg
     2701 gcgcagcccg cgcgacatcg ttgccggcgg cctgccgcgc ggcaaggcga ttggcgcgct
     2761 gaccgcgccg cccgccgtgc cggccggcct gccttccagc ctgctcaccc gccgcccgga
     2821 cttgcaggcg gccgagcaaa acctggtggc ggccaatgcc cgcatcggcg tggcgcgcgc
     2881 ggctttcttg cccagcatct cgctgaccgg cctgctgggc tcggaaagcg tggcgctgaa
     2941 caatctgttc aacggcccgg ccgccacctg gagctttgcc gccaatctgg ccatgccgct
     3001 gtttgacttt ggcaaaaccg ccgccggggt ggacgccgcc agcgcgcggc aacgccaggc
     3061 gctggcgcaa taccagctgg ccatccagac cgcctttaaa gaggcgcagg atgcgctggt
     3121 ggcgcaccgc aaaacccgcg aagccgccga ggcgcgcgcc acccaggtgg gcgcgctcaa
     3181 gcgctcgctg cggctggcgc ggctgcgcta cgacaatggg tattccagct atctggaagt
     3241 gctggacgcc gagcgtagcc tgtttcaggc cgagctggat gccatcgacg cccaacgcca
     3301 gcgcctgagc gcgacggtgg agttgtataa ggcgctgggc ggcggctggc aggcggataa
     3361 agccgacaag tcgtaaaccc ccgcacggct ggccggatga tgccagcccg cccatgtcat
     3421 gccaggcccg ccttgcgcgc gcctggcatg tttttttctt gtactttcat ggtcttgcca
     3481 catggccttg gtgaagaggc tttcgctggg caggctaaac cggccagctt ggcggatgaa
     3541 atccaggcat caagcaaatg agaatggttt gtaattcttt gaaattcaat ggcttggctg
     3601 cggttttcac ttggacatgc cccacgctgc acctacaata cgccacaggg gcaagccagc
     3661 cgacgttcgc aagccagccc gtttacgaca acttatgtgg agaacgccat gaagggcgac
     3721 aacacaatca tcgacattct caatgagctt ctggctggcg agctgaccgc tgtcgatcaa
     3781 tacctgatcc acggcgaaat gtacgctgac atgggcctgc acgcgctggc ggaaaaaacc
     3841 atccacgaat ccgagcacga gcgccagcac gcccgcgcgc tgattcagcg catcctgttc
     3901 ctggaaggca cgcccaatct ggccaagcgc gagccgctgc atgttggcca gaccgtgccg
     3961 gagatgctgc aagccgatct ggacgtggaa tacaaagtcg acgccgcgct caagcaagca
     4021 attgccgcct gtgaggccgc acaggactac gtcagccgcg acatgctgca agtgcaactg
     4081 gaagacaccg aggtggatca cgcctacttc ctggaaaaac aactgcgcct gatcaagctg
     4141 gtgaccctgc ccaactacct gcaaagccag atgggttctg gcagccccac ctgacgcaag
     4201 gagccacaca tgcaaggcga caaacaagtc atcgacaccc tgaacaaggt gctgcgcaac
     4261 gagctgaccg ccatcaacca gtatttcctg cacgcgcgga tgttccgcaa ctggggcctg
     4321 gaaaagctca acgactacca atacaagcaa tccatccggg tgatgaaaga agccgacgag
     4381 ctgatcgaac gcattctgtt cctggaaggc ctgcccaatc tgcaagacct gggcaagctg
     4441 ttgattggcg agcacgtgga agaatgcctg caaggcgacc tcacctacga acgcgacatt
     4501 cagcgcccgc tcctggtgca ggccatcgcc ctgtgcgaaa ccgccggcga ctatgtcagc
     4561 cgcgagctgc tggaagacct gctggagcac tgtgaagaag ccatcgactg gctggaaacc
     4621 cagcacagcc tgatcgccaa tgtgaccttg ccgaattacc tgcaagcgca tatggacgac
     4681 tgagcgccgc tcaccaaacc ctcctggcgt tgtggtgtga ccttgccgta ctacccgcac
     4741 tgtctgcaca cacttggcca ggagcgatgc gctgggtttt gtgagtgacg ttaagccgct
     4801 cacggctgca tcgctccacc ctcggccccg gcccgccttg cgcgcgccgg ggccgatttc
     4861 ctcctggcgt gcttgtcgcg tcttgtcaca cgcttggccc gcactgctgc gctgagcttt
     4921 ctgcgcgggt ttacgccggg tgcgaaattt ccccaatagc ccgcagccgc cacctgcttt
     4981 tatcatgcgg gctgcattcc cccacaggag ccccactgat gttctcttcc ctgcgccccc
     5041 tggccctggc tgccgccctg ctgcccagcc tggcttttgc ccaattcagc ctgccgccgc
     5101 tgccctatgc ggccgacgcg ctggagccgg tgatcgacaa gaccaccatg gaaatccacc
     5161 acgatcgcca ccacgccgcc tatgtgaaca acctcaacgc ccagatcaag agctatcccg
     5221 agctggatca cctcagcctg gacgcgctgc aaggcaagat cagccagtac aacgctgccg
     5281 tgcgcaacaa tggcggcggc cactacaacc atgatctgtt ctggaaggtg atggccgagc
     5341 ccggcctggg cggcgagccc agcgcggcgc tgaaacaggc catcgacgcc cagttcggca
     5401 atctggacga gctgaaaaaa cgctttgacc aggccgccgt cagccgcttt ggctcgggct
     5461 gggcctggct ggtggtgacc gccgacaaaa aactggccat cacctccacc gccaatcagg
     5521 acaacccgct gatggacctg ccggagattg cccagcgcgg cgcgccgatt ctggcgctgg
     5581 acgtgtggga gcacgcctac tatctgaaat accagaacaa gcgcgccgac tatgtcaccc
     5641 aatggtggaa agtggtgaac tggaatgagg tcagccgccg ttacgcggcg gccttgcgtt
     5701 aagcgcggct cacaaca
//
LOCUS       DAOPYX010000200         5666 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_17813_length_5666_cov_8.154518, whole genome shotgun sequence.
ACCESSION   DAOPYX010000200 DAOPYX010000000
VERSION     DAOPYX010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 5666)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5666)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5666
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17813_length_5666_cov_8.154518"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            284..1459
                     /locus_tag="PKN55_10205"
     CDS             284..1459
                     /locus_tag="PKN55_10205"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HNX71660.1"
                     /translation="MNDFAHVPLGRCSATLYHALSTSGWDDGRVKLAACLLKLVHDPD
                     SDADLILHEVGELTPEWAAHLQIHQDDPHASAVDSHALAAMLDDHAHRHGSDAWRSAG
                     LLEALLTHPALCAEGRALLPVLSATPPGHRHHVFALAMARSDENTPPSRFELDDPAYQ
                     RAYRLSAPLEVRALLAEAVRQQALVRLYPDGEDAALNACWQALDEDHLSLQLRDHPTS
                     LAALLAAPRVVADSTVSKIKVQFALLAPTIHHHGEQLWLQAPLPDSVLRLQRRDHYRL
                     KLGRAGSLHCQLELWLSDARGHRHRVQVQVQDLSSGGVGFVMDASQPELAPGTRLDDC
                     QLMLDDGCHRVDLLVCNRAHRPPPRPAWHYGCEFLNPPPPLVAQLDHEIMRQTALLR"
     gene            1511..2296
                     /gene="nudC"
                     /locus_tag="PKN55_10210"
     CDS             1511..2296
                     /gene="nudC"
                     /locus_tag="PKN55_10210"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011136452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HNX71661.1"
                     /translation="MPFDITSPTRPAQPGDVCVIWRDQRLCLPAHGGLPRVGEAALTS
                     LRHLGELDGAPCYVGELVGAPPADWRETSLRAALAGQTPPDWAPLLARASMLRQFDRS
                     HRYCSACATPLHDHGHDHGKVCPSCGLLAYPKLSPAMMALVVRGRQLLLARSPHFPPG
                     VYSALAGFVEPGETLEACVHREVQEEVGVRIDNLRYVGSQGWPFPHSLMLAFIADYVD
                     GDITPQPGEIESACWFDLDALPELPSQASIAHWLIHRAVDILR"
     gene            2305..3639
                     /locus_tag="PKN55_10215"
     CDS             2305..3639
                     /locus_tag="PKN55_10215"
                     /inference="COORDINATES: protein motif:HMM:NF016196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 domain-containing protein"
                     /protein_id="HNX71662.1"
                     /translation="MSSPSAAPLADWLARHRGGVSLSLAAGSLLALEALHAAGWLTHP
                     AWQIARHAAEGATVGGLADWFAVSALFHRIPIPWLSRHTNIVVNSRARITAGIVEMVQ
                     TQWLSPAAIRERLASVQLADALLALLNQPRHRQRALRIARRLALRLTEQLDSPALAGF
                     IDTQLRARLADADWAQPLGRLLAHALEHDGRAAVWETLVRLLDQALASQSVRESTARL
                     ALEALRDYEEDNWWRKAKTWLGKTQLQGDNDEEKLDLLTARIVGWLREELTRLRAEPE
                     HPLRQRLEQLLTRLAERLRAGDPALTAALAANQRQWLHSLDTTPLVASLLFSVRSGVQ
                     RQWRARHSELNTLLANLIDDALAGLAADAALRDKLDGWCKQVIHHFVEQNHALIGDTV
                     RLSLSPERLPDAQLVAQIESRVGEELQWIRVNGALVGGVAAGLIAALRLLLS"
     gene            3675..4127
                     /locus_tag="PKN55_10220"
     CDS             3675..4127
                     /locus_tag="PKN55_10220"
                     /inference="COORDINATES: protein motif:HMM:NF013487.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HNX71663.1"
                     /translation="MNKLLSLILTVAALAAPALSHAADPAATAEARVAAFKKMLRTFE
                     PMGTVVRERDPYRKDAFAEQAKTLQMLAREPWPLFVPGTAVGKSRAKPEIWSQPDAFK
                     KEQQGMIVAVDALAASAQAGDIKDIRAKYATLANTCKSCHDSFRAPQR"
     assembly_gap    4216..4315
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4445..5005
                     /locus_tag="PKN55_10225"
     CDS             4445..5005
                     /locus_tag="PKN55_10225"
                     /inference="COORDINATES: protein motif:HMM:NF006739.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HNX71664.1"
                     /translation="MNKIGMFFGSETGTTRLVAKKIHKKLGEALCDKPVNVNRVTLAD
                     LLRYDALILGTPSYGEGDLPGKSVGCFEPNWEEFFAQGPAEADFSGKHIAFFGLGAQE
                     RYADRFASSLRPLVDKFRAWGAEVVGDWPLDGYRYDYSAAEVDGRFLGLIIDQRTQGM
                     LTDERIDAWLAQVTPLLQAALTGDGA"
     gene            5002..5313
                     /locus_tag="PKN55_10230"
     CDS             5002..5313
                     /locus_tag="PKN55_10230"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HNX71665.1"
                     /translation="MSWLRISASEALTLLAQDDATVFDVRDSAAYQAGHLAKAANLAM
                     ERIPAWSKRLNKDAPVLVYCYHGNSSQEYAQMLADFRFNRVYSVDGGFAALLDAGAAL
                     A"
     gene            5310..>5666
                     /locus_tag="PKN55_10235"
     CDS             5310..>5666
                     /locus_tag="PKN55_10235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX71666.1"
                     /translation="MNTRADVAAWAAATPLSASDVDCATTVMLKILDGKCKMAEREKH
                     IMAWLYDALLGRPGQRFGQAEHALIAQARAGMDEALRQTVYERRVLAETTLSRPVMKT
                     FKAMIRAQGLFAGEAGE"
BASE COUNT          962 a         1886 c         1768 g          950 t
ORIGIN      
        1 ggcgcagctg ctcaatggcc tgctggcagc aatgcagggc cgctggccaa ttgccagcgc
       61 gcagcaggcc cagggcctgg cggtacaagg tatcgggaga ggcaggcaag acagtgtcag
      121 tcatgagcgg tatcggtgcg gcaaacagtg ttgccatgca gtatacctgc cgatgccgtc
      181 tgactggcaa ttcagcaaat atcagtcata ctcaggccat aacatgccat agtaatgaaa
      241 ttgccgccct caacctgaat atgtcagcaa ggatctgctc accatgaatg attttgcgca
      301 tgtcccgctt ggccgttgca gtgccaccct gtaccacgct ttgtctacct ccggctggga
      361 tgacgggcgg gtgaagctgg cggcctgttt gctcaagctg gtgcacgacc cagacagtga
      421 cgccgatctc atcctgcacg aggtagggga gctgaccccg gaatgggctg cccacctgca
      481 aattcaccag gacgatccgc acgcctcggc ggtggacagc catgccctgg ccgccatgct
      541 ggacgatcac gctcaccgcc acggcagcga tgcctggcgc agcgctggcc tgctggaggc
      601 actgctgacc catccggcct tgtgtgccga gggccgtgcg ctgctgccgg tgctgagcgc
      661 caccccgccc ggccaccgcc accatgtgtt tgcgctggcc atggcccgct cggacgaaaa
      721 caccccgccc agccgctttg aactggacga tccggcctat cagcgcgcct accggctatc
      781 cgccccgctg gaggtgcgcg ccctgctggc tgaggctgtc cggcagcagg cgttggtgcg
      841 tttgtatccc gatggtgagg acgctgcgct gaatgcctgc tggcaggcgc tggatgaaga
      901 ccatctcagc ctgcaattgc gggatcatcc cacaagcctg gcagccctgc tggcggcgcc
      961 acgggtggtg gcggacagca cggtcagcaa aatcaaggtg cagtttgccc tgttggcccc
     1021 cacgattcat caccatggtg agcagctgtg gctacaggcg cccttgcccg acagcgtgct
     1081 gcgcctgcaa cggcgcgacc actatcggct caagctgggg cgggccggca gcctgcattg
     1141 ccaattggaa ctgtggctga gcgatgcgcg cggccaccgc caccgtgtac aggtgcaagt
     1201 gcaggatctt agcagcggtg gtgtcggctt tgtcatggat gccagccagc ccgagctggc
     1261 gcccggcacc cgactggacg attgccagtt gatgctggac gacggctgtc atcgggtgga
     1321 tctgctggtg tgcaatcgtg cccaccggcc gccgccgcgt ccggcctggc actatggctg
     1381 tgaattcctg aatccgccgc cgccgctggt ggcgcaactg gatcacgaaa tcatgcgcca
     1441 gaccgccttg ctgcgctgat ccgccctgag cggtgtacac tgcgggtttt gctttctgcc
     1501 gccctgcccg atgccctttg acattaccag ccccacccgc ccggcacagc cgggcgatgt
     1561 ctgtgtgatc tggcgcgacc agcgcctgtg cctgcccgcg catggcggcc tgccgcgcgt
     1621 gggcgaggcg gcgctgacca gcttgcgtca tctgggagag ctggacggcg cgccgtgtta
     1681 tgtcggcgag ctggtgggtg cgccaccggc cgactggcgc gaaaccagcc tgcgcgccgc
     1741 gctggccggg caaaccccac cagactgggc gccgctgttg gcgcgcgcca gcatgctgcg
     1801 ccagtttgac cgcagccacc gctattgctc ggcctgcgcc acccccttgc acgaccacgg
     1861 ccacgaccat ggcaaggtct gccccagctg cggcctgctg gcctacccga aactctcccc
     1921 ggcgatgatg gcgctggtgg tgcgcggccg ccagctgctg ctggcgcgct cgccgcattt
     1981 tccgccgggg gtgtatagcg cgctggccgg ttttgttgag ccgggcgaaa cgctggaagc
     2041 ctgcgtgcac cgcgaagtgc aggaagaagt cggcgtgcgc atcgacaatc tgcgctatgt
     2101 cggctcgcag ggctggccgt ttccgcattc gctgatgctg gcctttattg ccgactacgt
     2161 ggacggcgac atcaccccgc agcccggcga aatcgaatcc gcctgctggt ttgacctgga
     2221 cgcgctgccc gagctgccca gccaggccag cattgcccac tggctgatcc accgtgcggt
     2281 ggacatcctg cgctgagccc ggccatgtcc tcgccctccg ccgccccatt ggctgactgg
     2341 ctggcccgcc atcgcggcgg cgtctcgctc agcctggccg ccggcagcct gctggcgctg
     2401 gaagcgctgc acgccgccgg ctggctgacg catccggcct ggcaaattgc ccgccacgcg
     2461 gccgaaggcg ccaccgttgg cggcctggcc gactggtttg ccgtgtcggc gctgtttcac
     2521 cgcatcccca ttccctggct gtcgcggcac accaatattg tggtcaacag ccgcgcgcgc
     2581 atcaccgccg gcattgtcga gatggtgcaa acccaatggc tgtcgccagc ggccattcgt
     2641 gaacggctgg cctcggtgca gctggccgac gcgctgctgg ccctgctgaa tcaaccgcgc
     2701 caccgccaac gcgcgctgcg cattgcccgc cggctggcgc tgcggctgac cgaacagctg
     2761 gattcgcccg ccctggccgg ctttatcgac acccaattgc gcgcccgcct ggccgacgcg
     2821 gactgggcac agccgctggg ccggctgttg gcgcacgcac tggagcacga cggccgcgcc
     2881 gccgtgtggg aaaccctggt gcggctgctg gatcaggcgc tggcctcgca aagcgtcagg
     2941 gaatccaccg cccggctggc gctggaagca ctgcgcgact acgaagaaga caactggtgg
     3001 cgcaaggcca agacctggct gggcaaaacc cagttgcaag gcgataacga cgaagaaaaa
     3061 ctcgacctgc tcaccgcccg catcgtcggc tggctgcgtg aagaactcac ccggctgcgc
     3121 gccgagcccg agcacccgct gcgccagcgg ctggagcaac tgctcacccg gctggccgag
     3181 cgcctgcgcg ccggcgaccc ggcgctgacc gccgccctgg ccgccaacca gcgccaatgg
     3241 ctgcacagcc tggacaccac gccgctggtg gccagcctgc tgtttagcgt gcgcagcggc
     3301 gtgcaacgcc aatggcgcgc ccgccattcg gaactcaaca ccctgttggc caatctgatt
     3361 gacgacgcgc tggccggcct ggccgccgac gccgccctgc gcgacaagct ggacggctgg
     3421 tgcaagcagg tgattcacca ttttgtcgaa caaaatcacg cgctgattgg cgacaccgtg
     3481 cggctgagcc tgtcgcccga gcgcctgccc gacgcccaat tggtggcgca gatcgaatcg
     3541 cgcgtgggcg aagagctgca atggatccgc gtgaatggcg cgctggtggg cggcgtcgcc
     3601 gccggcctga ttgccgcgct gcgcctgctg ctgagctgat tatttttgat ttttgcctgg
     3661 aaccgacccc aaccatgaac aagctgctgt ccctgatcct caccgtggcc gccctggccg
     3721 cccccgccct cagccacgct gccgacccgg ccgccaccgc cgaagcccgc gttgccgcct
     3781 tcaagaaaat gctgcgcacc ttcgagccga tgggcaccgt ggtgcgcgag cgcgacccct
     3841 atcgcaagga cgcctttgcc gaacaggcca aaaccctgca aatgctggcg cgcgaaccct
     3901 ggccgctgtt tgtgcccggc accgccgtgg gcaaaagccg cgccaaacca gaaatctgga
     3961 gccagcccga cgccttcaag aaagaacaac aaggcatgat cgtcgccgtg gatgcgctgg
     4021 ctgccagcgc ccaggccggc gacatcaagg acatccgcgc caaatacgcc accctggcca
     4081 acacctgtaa gagctgccac gacagcttcc gcgccccgca acgctaatat gtgatttttt
     4141 tgcctgcggt ttacccgcag gcagaatagc caatgccacg caaggcctga ggctggcgaa
     4201 agcgattccc ccctcnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnngctga
     4321 gtgtgggttt cttgacattc ggtggtctct agaaaaaatt ttccagcatc atcaatcata
     4381 tgcacgctgg caccatccct gcatgtggtc gccctgacag acatcaggat ggagcagacc
     4441 ccacatgaac aagatcggga tgttttttgg ttcggaaacc ggcaccaccc ggctggtggc
     4501 gaaaaaaatc cataaaaaac tcggcgaagc gctgtgcgac aaacccgtca acgtcaatcg
     4561 cgtcaccctg gccgacttgc tgcgctacga cgcgctgatt ctgggcacgc ccagctatgg
     4621 cgagggcgac ttgccaggca aaagcgtggg ctgttttgag cccaactggg aagagttttt
     4681 cgcccaaggg ccggccgagg cggatttcag cggcaagcac attgcctttt ttggcctggg
     4741 cgcgcaagag cgctacgccg accgctttgc cagctcgctg cggccgctgg tggataaatt
     4801 ccgcgcctgg ggcgctgagg tggtgggcga ctggccgctg gatggctacc gctatgatta
     4861 ttccgccgcc gaggttgacg gccgcttttt ggggctgatt atcgaccagc gcacgcaagg
     4921 catgctcacc gatgaacgca tcgacgcctg gctggcgcaa gtcaccccgc tgctgcaagc
     4981 ggcattgacg ggagacggcg catgagctgg ctgcgcattt ctgccagcga ggcgctgacc
     5041 ttgctggcgc aggacgacgc cacggtgttt gatgtgcgcg acagcgcggc ctatcaagcc
     5101 ggccacctgg ccaaggccgc caacctggcc atggagcgca tcccggcgtg gagcaagcgg
     5161 ctgaacaaag acgccccggt gctggtgtat tgctatcacg gcaacagcag ccaggaatac
     5221 gcccagatgc tggcggattt tcgttttaac cgggtgtaca gcgtggacgg cggctttgcc
     5281 gcgctgctgg acgccggcgc agcgctggca tgaacacccg cgccgacgtc gccgcctggg
     5341 ctgccgccac cccgctgagc gccagcgatg tggactgcgc caccacggtg atgctgaaaa
     5401 tcctcgacgg caaatgcaaa atggccgagc gggaaaaaca catcatggcc tggctgtacg
     5461 acgccttgct tggccgcccc ggccagcgct ttggccaggc cgaacacgcg ctgattgccc
     5521 aggcccgcgc cggcatggac gaagcactgc gccagacggt gtacgaacgc cgtgtgctgg
     5581 cggaaaccac gctatcccgc ccggtgatga aaaccttcaa ggcgatgatc cgcgcccaag
     5641 gtttgtttgc cggcgaggcg ggggaa
//
LOCUS       DAOPYX010000201         5661 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_17841_length_5661_cov_9.067963, whole genome shotgun sequence.
ACCESSION   DAOPYX010000201 DAOPYX010000000
VERSION     DAOPYX010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 5661)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5661)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5661
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17841_length_5661_cov_9.067963"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1139)
                     /locus_tag="PKN55_10240"
     CDS             complement(<1..1139)
                     /locus_tag="PKN55_10240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014086040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HNX71667.1"
                     /translation="MRVLLILNPGSATFKFEFFALADNGEATPRYRGLVDGVGRDSAR
                     LLIERISDNSVAEDRQIQPASHPDALAAVFAFAREHGYTLAAVTHRIVHGGPFFQRAV
                     RIDADVIAKLQTLTPLAPLHQPVGLAAIAAVSQADPDITQIACFDTAFHTTQDPLGAR
                     FAIAQRWHDAGVRRYSFHGLSYAAIAHRLPGVVGPELAEGRVAVLHLGSGSSACILHR
                     RQSVANSTGMSAVEGLMMGTRPGNLDPEVVLWWMENAGMDIAAVRRELYKASGLLGVS
                     GISADMRELLASSEPAAALAVELYCYRAAREIGALAVQAGGLDALVFTAGTGQRSAPV
                     REKILARLAWLGFDLDPEANAANAARITRDGSRLSAWVIATDEEG"
     gene            complement(1139..2071)
                     /locus_tag="PKN55_10245"
     CDS             complement(1139..2071)
                     /locus_tag="PKN55_10245"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012697920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HNX71668.1"
                     /translation="MTRDEHAFADILEEAEQFGPLPMAVAHPCSQESLMGAVEAAQHG
                     IITPILVGPQAKIAKVAAEFDVDLSRFRVIDVPHSHAAAETAVALVKNGEAHALMKGS
                     LHTDEFMTEVLKKDGGLRTGRRLSHVFIMRVDSYPRYLFITDAAINIQPDLMAKVDIT
                     QNAIDLTHALGIARPKVALLSAVETVTPTIQSTLDAAALCKMADRGQITGAILDGPLA
                     FDNAISAEAAHTKHIVSAVAGDPDILVVPDLESGNMLAKQLSYLAAAASAGIVMGARV
                     PIVLTSRAEDAQGRLASAAVANVVGHKLLNAGDD"
     gene            complement(2082..2858)
                     /gene="fabI"
                     /locus_tag="PKN55_10250"
     CDS             complement(2082..2858)
                     /gene="fabI"
                     /locus_tag="PKN55_10250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008953355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004318 - enoyl-[acyl-carrier-protein]
                     reductase (NADH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HNX71669.1"
                     /translation="MENLKTMLQGKKGLIVGIANDNSIAYGCARVLRDLGAEFAVTYL
                     NEKADRFVRPLAEELESPIITQMNVENPGELEATFKAIEEKWGKLDFVIHSIAFCPMD
                     DLHGRVTDCSKDGFLQAMRVSCYSLIEMARLAEPLMKDGGALVTMTYYGADKVVENYN
                     VMGPVKAALESTVRYLAAELGSKKIRVHAVSPGPLKTRAASGIAHFDKLIEEAIARTP
                     EHRLVDIEDVGMTTAFLVSGGARALTGDTIYVDGGFHNMA"
     gene            3243..3488
                     /locus_tag="PKN55_10255"
     CDS             3243..3488
                     /locus_tag="PKN55_10255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008953354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HNX71670.1"
                     /translation="MPIYEYRCEDCGQVKEHLQKLSDAPIAECPACGGSHYTKLLSAG
                     GFQLKGSGWYATDFKHSQKKPELPPCAASGCGGGCAA"
     gene            3485..4129
                     /locus_tag="PKN55_10260"
     CDS             3485..4129
                     /locus_tag="PKN55_10260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017508125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HNX71671.1"
                     /translation="MTQGKLTLKACLLTGVLIWLPFAVTLWVLDMVISTMDSMINWLP
                     MALQPTHWFGMHIPGSGLVLALLIVLLTGFLAANMLGQKVVGLWEAMLGRIPVVKTIY
                     NSVKQVSDTLLSGSGQAFSKALLVQFPHQDAWTIAFLTGKPGPGVAEHLNGDYVSVYV
                     PTTPNPTSGYMMLVARSAVRELDMSVDDALKYIISMGVVAPAAKPVPPSTPTSR"
     gene            4146..>5661
                     /gene="aspS"
                     /locus_tag="PKN55_10265"
     CDS             4146..>5661
                     /gene="aspS"
                     /locus_tag="PKN55_10265"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HNX71672.1"
                     /translation="MRTDYCGLIDKKFLGQTVTVKGWAHRRRDHGGVIFIDLRDREGL
                     VQVVIDPDTPEAFQTADASRNEYVLAITGIVRERPAGTANANLISGEIEILAKQIEVL
                     NTAATPPFQIDDENLSENVRLTNRVIDLRRPVMQKNLRLRYRVAMGVRNHLDKQGFID
                     IETPMLTRSTPEGARDYLVPSRVHPGEFFALPQSPQLFKQLLMVAGFDRYYQITKCFR
                     DEDLRADRQPEFTQIDIETSFLNEDEIMDITETMAQEIFRDVLSVELGKFPRMTYNDA
                     MFYYGSDKPDLRVSLKFTELTDLMKTEEFKVFRGAADMPNGRVVALRVPGGASLSRKE
                     IDDYTQFVSIYGAKGLAYIKVNDNSKITNDETSGLQSPIVKFLSVNALSEIIARTGAE
                     NGDLIFFGADKAKVVNEAIGALRIKIGHERGEAGGYFVREWRPLWVVDFPMFEYDEDD
                     DRWTACHHPFTSPKPGHEDLMDTDPGQCLARAYDMVLNGWEIGGGSVRIHRADVQ"
BASE COUNT         1023 a         1818 c         1787 g         1033 t
ORIGIN      
        1 tgcccttctt cgtcggtggc gatcacccag gccgacaggc ggctgccgtc gcgggtgatg
       61 cgcgcggcgt tggcggcgtt ggcctcgggg tcgaggtcaa aacccagcca ggccaggcgg
      121 gcgaggattt tctcccgcac tggcgcgctg cgctggccgg tgccggcggt gaacaccagc
      181 gcgtccaggc cgccggcctg caccgccagc gcgccgattt cacgcgcggc gcggtagcaa
      241 tacagctcca ccgccagcgc ggcggccggt tcgctggagg ccagcagctc gcgcatatcg
      301 gcagaaatgc ccgacacgcc caacagcccg ctggccttgt acaactcgcg gcgcacggcg
      361 gcgatgtcca tgccggcgtt ttccatccac cacaacacca cttccgggtc gagattgccg
      421 gggcgggtgc ccatcatcag gccttccacc gcgctcatgc cggtgctgtt ggccacgctt
      481 tgccgacggt gcaggatgca cgccgacgag ccgctgccca gatgcagcac cgccacccgc
      541 ccctcggcca gctcggggcc gactacgcca ggcagacggt gggcaatcgc cgcataagac
      601 agcccatgaa agctgtagcg gcgcacgccg gcgtcgtgcc agcgctgggc gatggcaaag
      661 cgtgcgccca gcgggtcttg cgtggtgtga aacgcggtgt caaagcaggc gatctgggtg
      721 atgtccgggt cggcctggct gacggcggca atcgccgcca gccccaccgg ctggtgcagc
      781 ggcgccagcg gggtgagggt ttgcagcttg gcgatgacat cggcgtcgat gcgcacggcg
      841 cgctggaaga acggcccgcc gtgcacaatg cggtgggtga ccgccgccag cgtgtaacca
      901 tgctcgcggg caaaggcaaa caccgccgcc agcgcgtccg ggtggctggc cggctggatc
      961 tggcgatctt ccgccacgct gttgtcgctg atccgctcga tcagcagccg ggcgctgtcg
     1021 cgcccgacgc cgtccaccag gccgcgatag cgcggggtgg cctcgccatt gtcggccagg
     1081 gcgaaaaatt caaacttgaa cgtcgccgag ccggggttga ggatcaacaa cacgcgcatc
     1141 aatcgtctcc ggcgttcaac agcttgtggc cgaccacgtt ggccaccgcc gccgaggcca
     1201 gacggccttg ggcgtcttcg gcgcggctgg tcagcacgat gggcacgcgc gcgcccatca
     1261 caatgccggc cgaggccgcc gccgccaggt aggacaactg cttggccagc atattgcccg
     1321 attccagatc cggcaccacc aggatgtccg ggtcgcctgc cacggcggag acaatgtgct
     1381 tggtgtgggc ggcttcggcc gagatggcgt tgtcaaaggc cagcgggcca tccagaatcg
     1441 cgccggtgat ctggccgcga tcggccatct tgcacagcgc ggcggcgtcc agggtggact
     1501 ggatggtcgg ggtgacggtt tccaccgccg acagcagcgc caccttgggc cgggcgatgc
     1561 ccagcgcgtg ggtcaggtcg atggcgttct gggtgatgtc caccttggcc atcagatccg
     1621 gctggatatt gatggcggcg tcggtgatga acagataacg cgggtagctg tccacgcgca
     1681 tgatgaacac atgcgacagg cgacggccgg tgcgcaggcc gccgtctttt ttcagcacct
     1741 cggtcatgaa ttcgtcggtg tgcaggctgc ccttcatcag cgcgtgggct tcgccattct
     1801 tcaccagcgc cacggcggtt tcggccgcag cgtggctgtg cggcacgtca atcacccgaa
     1861 agcgcgacag gtccacgtca aattcggcgg ccaccttggc gatcttggcc tgcgggccga
     1921 ccagaatcgg ggtgatgatg ccgtgctggg cggcctcaac ggcgcccatc agcgattcct
     1981 ggctgcacgg gtgcgccacc gccatcggca gcgggccaaa ctgttcggct tcttccagaa
     2041 tgtcggcaaa agcgtgttcg tcgcgggtca tggtgacctc cttacgccat gttgtggaag
     2101 ccgccatcga catagatggt gtcgccggtc agcgcgcggg cgccgccaga caccaggaag
     2161 gcggtggtca tgcccacgtc ttcgatgtcc accaggcggt gttccggggt gcgggcgatg
     2221 gcttcttcaa tcagcttgtc aaagtgggcg atgccagagg cggcgcgggt tttcagcggg
     2281 ccgggggaca cggcgtgcac gcggattttc ttgctgccca gctcggcggc cagatagcgc
     2341 acggtggatt ccagcgcggc cttcaccggg cccatcacat tgtagttttc caccactttg
     2401 tcagcgccgt agtaagtcat ggtcaccagc gcgccgccgt ctttcatcag cggttcggcc
     2461 aggcgggcca tttcgatcag cgagtagcac gacacgcgca tggcttgcag gaagccatcc
     2521 ttggaacaat cggtcacgcg gccatgcagg tcgtccatcg ggcagaaggc gatcgagtgg
     2581 atgacaaaat ccagcttgcc ccatttttct tcgatggcct tgaaggtggc ttccagttcg
     2641 cccgggtttt ccacgttcat ctgggtgatg atcgggcttt ccagctcttc ggccagcggg
     2701 cggacaaaac ggtcggcttt ttcgttcagg taggtgacgg caaactcggc gccgaggtca
     2761 cgcagcacgc gggcgcagcc gtaggcaatc gaattgtcgt tggcgatgcc cacgatcagg
     2821 ccttttttgc cttgcagcat ggtcttgagg ttttccatgg tcgagctccg tatcgttgtt
     2881 gcgcccatct gcttaagctg ggcatgaggt tgagtcatcc tgcgtcgccg cccggattgg
     2941 gcaggccagg gagtgggagg gccgccgcca gcaagaatca agcggccgat tgcatcatgt
     3001 tgccgtgcaa cataacctga atgcaagtgt ggcttttctt ttttatccca cagtgggatg
     3061 acaagagcgt gaaagcctgc gccgtcacgg tgcatgccgc gcaatgtttt ggtttggcgt
     3121 ggcggtgcga tatacttttg tgtttgttcg tgggccggcg cttgcctgcc ttgcgactgc
     3181 cggactgtaa caaaagttcg gcgtttcatg tcattgactt gcgtttgcgg agactttacc
     3241 cgatgccaat ctacgaatac cgttgcgaag actgtggtca ggtcaaggag catttgcaaa
     3301 aactgtcgga tgcgccgatt gccgaatgcc cggcctgcgg cggcagccac tacaccaagc
     3361 tcttgtctgc tggcggtttt cagctcaagg gcagcggctg gtacgccact gacttcaagc
     3421 acagccagaa aaaacccgag ctgccgccgt gtgcggccag cggctgcggc ggcggttgcg
     3481 cggcatgacc cagggcaaac tcaccctcaa ggcctgcctg ctgaccggcg tgctgatctg
     3541 gctgccgttt gccgtcacgc tgtgggtgct ggacatggtg atttccacca tggacagcat
     3601 gatcaactgg ctgccgatgg ccttgcagcc cacccactgg tttggcatgc acattcccgg
     3661 cagcgggctg gtgctggcgc tgctgattgt gctgcttacc ggctttctgg ccgccaatat
     3721 gctgggccag aaagtggtgg gcctgtggga ggccatgctg ggccgcatcc cggtggtgaa
     3781 aaccatctac aacagcgtca agcaagtcag cgacaccttg ctgtccggca gcgggcaggc
     3841 gttttccaag gctttgctgg tgcagtttcc gcaccaggac gcctggacca tcgcctttct
     3901 caccggcaaa cccggccccg gcgtggccga gcacctcaat ggcgactacg tcagcgtcta
     3961 cgtgcccacc acccccaacc cgacctccgg ctacatgatg ctggtggcgc gctcggcggt
     4021 gcgcgagctg gacatgagcg tggacgacgc gctgaagtac attatttcga tgggcgtggt
     4081 ggcccctgcc gccaagcccg tcccaccttc tactcctacc tctcgttgaa agttgaacac
     4141 agagcatgcg aaccgactac tgcggactga ttgacaaaaa attccttggc cagacggtca
     4201 ccgtcaaggg ctgggcgcac cgtcgccgcg accacggcgg cgtgatcttc atcgacctgc
     4261 gcgaccgcga aggcctggtg caagtggtga tcgacccgga caccccggaa gccttccaga
     4321 ccgccgacgc ttcgcgcaat gaatatgtgc tggccatcac cggcatcgtg cgcgaacgcc
     4381 cggccggcac cgccaacgcc aacctgattt ctggcgaaat cgaaattctc gccaaacaaa
     4441 tcgaagtgct caacaccgcc gccaccccgc cgttccagat cgacgacgaa aacctctcgg
     4501 aaaacgtccg cctgaccaac cgggtgatcg acctgcgccg cccggtgatg caaaaaaacc
     4561 tgcgcctgcg ctaccgcgtg gccatgggcg tgcgcaacca cctggacaag cagggcttca
     4621 tcgacatcga aaccccgatg ctgacccgct ccaccccgga aggcgcccgc gactacctgg
     4681 tgccctcgcg cgtgcatccg ggtgaattct ttgccctgcc gcaatcgccg cagctgttca
     4741 agcagctgct gatggtggcc ggctttgacc gctactacca aatcaccaaa tgcttccgcg
     4801 acgaagacct gcgcgccgac cgtcagccgg aattcaccca gattgatatc gaaacctcgt
     4861 tcctgaacga ggacgaaatc atggacatca ccgagaccat ggcgcaggaa atcttccgcg
     4921 acgtgctcag tgtcgagctg ggcaagttcc cgcgcatgac ctacaacgac gccatgttct
     4981 actacggctc ggacaagccc gatctgcgcg tgtcgctcaa gttcaccgaa ctgaccgacc
     5041 tgatgaagac cgaagaattc aaggtgttcc gtggcgcggc cgacatgccc aatggccgcg
     5101 tggtcgccct gcgcgtgccc ggcggcgcca gcctgtcgcg caaggaaatc gacgactaca
     5161 cccagtttgt cagcatctac ggcgccaagg gcctggctta catcaaggtc aacgacaaca
     5221 gcaagatcac caacgacgaa accagcggcc tgcaatcgcc catcgtcaag ttcctgtcgg
     5281 tgaacgcgct gagcgaaatc atcgcccgca ccggcgccga aaacggcgac ctgatcttct
     5341 ttggcgccga caaggccaag gtggtcaacg aagccatcgg cgcgctgcgc atcaagatcg
     5401 gccacgaacg cggcgaagcc ggcggctatt ttgtgcgcga atggcgtccg ctgtgggtgg
     5461 tggacttccc gatgtttgaa tacgacgaag acgacgaccg ctggaccgcc tgccaccatc
     5521 cgttcaccag cccgaaaccg ggccatgaag acctgatgga caccgatccg ggccagtgcc
     5581 tcgcccgcgc ctacgatatg gtgctgaacg gctgggaaat cggcggcggc tcggtgcgta
     5641 tccaccgcgc cgacgtgcag g
//
LOCUS       DAOPYX010000202         5642 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_17922_length_5642_cov_8.474673, whole genome shotgun sequence.
ACCESSION   DAOPYX010000202 DAOPYX010000000
VERSION     DAOPYX010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 5642)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5642)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5642
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17922_length_5642_cov_8.474673"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..552
                     /locus_tag="PKN55_10270"
     CDS             <1..552
                     /locus_tag="PKN55_10270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008953608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HNX71673.1"
                     /translation="AKNLGVADGDVAKMRDEISKNVTREAKRRLQARVKEGVMQALLD
                     VTPIELPKSLIDMEIGRLMQQAREDMMQRGYGAQMKDMPLPRELFEEQAKRRVSLGLI
                     LAEVVEKHSLSATPEQVKAFITEQAESYENPQEVIDWYYASPERLDGPASVVLEDNVV
                     AFVLSQAKVNEQAVPFEELMGNN"
     gene            552..1193
                     /gene="clpP"
                     /locus_tag="PKN55_10275"
     CDS             552..1193
                     /gene="clpP"
                     /locus_tag="PKN55_10275"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017509551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HNX71674.1"
                     /translation="MHTLPGWLNNMSGPQNLGLVPMVVEQSGRGERAFDIYSRLLKER
                     IVFLVGPVTDESANLVVAQLLFLESENPDKDISLYINSPGGSVTAGMSIYDTMNFIKP
                     DVSTLCIGQACSMGAFLLSAGAKGKRFSLPNSRVMIHQPLGGYQGQASDIEIHAKEIL
                     YLKERLNKMLADHTGQPLEVIERDTDRDNFMSADAALAYGLVDKVLPNRQAAA"
     gene            1205..2482
                     /gene="clpX"
                     /locus_tag="PKN55_10280"
     CDS             1205..2482
                     /gene="clpX"
                     /locus_tag="PKN55_10280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011136104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HNX71675.1"
                     /translation="MSDNKSSDKLLYCSFCGKSQHEVKKLIQGPQVYICDECVELCND
                     ILHEEQITSPAPGEPGEGKRAPLPTPHQLRGTLDQYVIGQDGAKKALAVAVYNHYKRL
                     ETKLAPGDVELAKSNILLIGPTGSGKTLLAQTLARLLDVPFVMADATTLTEAGYVGED
                     VENIIAKLLQKCDYDVDRAQRGIIYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLV
                     EGTVASIPPQGGRKHPNQEFIQVDTTNILFICGGAFDGLEKIIRNRSQKGGIGFGADV
                     YSKDDGRAVGKVLQETEPEDLIKFGLIPEFIGRLPVIAMLDELDEEALVTILTEPKNA
                     LVRQFQKLFEMEQTELEIRPSALRAIAKQALARKTGARGLRSILERALLDTMYDLPSL
                     QNVVQVVVDETVIDKGSAPLLIHADAEPVAQSA"
     gene            2649..5069
                     /gene="lon"
                     /locus_tag="PKN55_10285"
     CDS             2649..5069
                     /gene="lon"
                     /locus_tag="PKN55_10285"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012697814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HNX71676.1"
                     /translation="MSTTELLTEDTSLPLLPLRDVVVFPHMVIPLFVGRPKSIKALEK
                     AMESDKQILLLAQKSASKDEPSADDLYALGTVATVLQLLKLPDGTVKVLVEGRQRASV
                     NHINEDAGYFVGDITPLPAEGKENTETEAMRRALIGQFEQFVKLNKKIPPEILSSLSA
                     IDKAGRLADTIAAQLPLKLEQKQDVLEMADIKTRLEHLLAQLESEIDILQVEKRIRGR
                     VKRQMEKSQRDYYLNEQVKAIQRELGEEDESSDLDELEKKIKTAGMSKEARDKANSEL
                     RKLRMMSPMSAEATVVRNYIETLIELPWKKKTKISKDLEHAETVLDEDHFGLDKVKER
                     IVEYLAVQQRVDKLKAPILCLVGPPGVGKTSLGQSIARAVNRQFVRMALGGVRDEAEI
                     RGHRRTYIGSMPGKVLQNMTKVGVKNPLFLLDEVDKMGADFRGDPSSALLEVLDPEQN
                     ATFVDHYAEVEYDLSDVMFVATANSMNIPPALLDRMEIIRLSGYTEDEKIAIAERYLL
                     PKQMKNTGLSEAELAVQESAVRDIVRYYTREAGVRSLDRELNKICRKVVKAQQGKKAA
                     AKPTRVTVNAKNLDKYLGVRRYDYGLAEEANQVGQVTGLAWTEVGGELLTIEAVGLPG
                     KGTIIRTGQLGEVMQESITAAMSVVRRRARSLGIAKDFYEKNDIHIHLPEGATPKDGP
                     SAGIGIVTAITSSLTGIPVRADVAMTGEITLRGEVLPIGGLKEKLLAAQRGGIKHVLI
                     PKGNIKDLAEIPDSIKKKLEIHPVKWIEEVFALALEHSPTPLVDEDDVGTIPAKADEL
                     PAARPVTH"
     gene            5257..5547
                     /locus_tag="PKN55_10290"
     CDS             5257..5547
                     /locus_tag="PKN55_10290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009426680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HNX71677.1"
                     /translation="MLVKGTTVNKSELIDAMAASADIPKAAAGKALDGMVEALTQALK
                     NGDTVTLVGFGSFYVGERAERTGRNPKTGETITIPAAKSPKFRAGKTLKDAL"
BASE COUNT         1281 a         1644 c         1698 g         1019 t
ORIGIN      
        1 gccaagaacc tgggcgtggc cgacggcgac gtggccaaga tgcgtgacga aatcagcaag
       61 aacgtcaccc gcgaagccaa gcgccgcctg caagcccgcg tcaaggaagg cgtgatgcag
      121 gccctgctgg acgtcacccc gatcgaactg cccaagtcgc tgatcgatat ggaaatcggc
      181 cgtctgatgc aacaggctcg tgaagacatg atgcagcgcg gctatggcgc ccaaatgaag
      241 gacatgccgc tgccgcgcga actgtttgaa gagcaagcca agcgtcgcgt gtcgctgggc
      301 ctgattcttg ctgaggtggt ggagaagcac tcgctgagcg ccaccccgga acaagtcaag
      361 gctttcatca ccgagcaggc tgaaagctac gaaaacccgc aggaagtgat cgactggtac
      421 tacgccagcc cggaacgcct ggacggcccg gcctcggtgg tgctggaaga caatgtggtg
      481 gcttttgtgc tgtcgcaggc taaagtcaac gaacaagccg ttccgtttga agaattgatg
      541 gggaataact gatgcataca ctgccaggtt ggctgaacaa tatgtcgggg ccgcagaatc
      601 tgggcctcgt tccgatggtt gtcgagcaaa gtggtcgtgg cgaacgcgcc tttgacattt
      661 actcgcgcct gctcaaggag cgcatcgtgt tcctggtcgg gccggtaacc gatgaatcgg
      721 ccaatctggt ggtggcgcag ctgctgtttc ttgaatcgga aaacccggac aaggacatct
      781 cgctgtacat caattcgccg ggcggctcgg tgactgctgg catgtcgatc tacgacacga
      841 tgaatttcat caagccggat gtctccacct tgtgcatcgg ccaggcgtgc agcatgggcg
      901 ccttcctgct gtcggccggc gccaagggca agcgcttctc gctgcccaat tcgcgggtga
      961 tgattcatca gccgctgggc ggctatcagg gccaggcctc ggacattgaa atccacgcca
     1021 aggaaatcct gtacctgaag gaacgcctga acaagatgct ggccgatcac accggccagc
     1081 cgctggaggt gatcgagcgc gacaccgacc gcgacaattt catgagcgct gacgccgccc
     1141 tggcctatgg cctggtggac aaagtgctcc ccaaccggca agccgccgcc taacggatca
     1201 agtgatgtct gacaataaat ccagcgacaa gctgctgtac tgctcgttct gcggcaagag
     1261 tcagcatgaa gtcaaaaagc tgattcaggg cccgcaggtg tatatctgcg acgaatgcgt
     1321 cgagctgtgc aacgacatcc tgcatgaaga gcagatcacc tcgcccgccc ctggcgagcc
     1381 gggtgagggc aagcgcgcgc cgctgcccac gccgcatcag ctgcgcggca cgctcgacca
     1441 gtacgtgatt ggccaggatg gcgccaaaaa agcgctggca gtggcggtat acaaccacta
     1501 caagcgcctg gaaaccaagc tggcgccggg cgatgtcgag ctggccaaga gcaatatttt
     1561 gctgattggc ccgaccggct ccggcaaaac cttgctggcg caaaccctgg cccgcctgct
     1621 ggatgtgcct tttgtgatgg ccgacgccac cacgctgacc gaagccggct atgtgggcga
     1681 agatgtggaa aacatcatcg ccaagctgct gcaaaagtgc gactacgatg tggaccgcgc
     1741 ccagcgcggc atcatctata ttgacgaaat cgacaagatt tcgcgcaagt cggacaaccc
     1801 gtccatcacc cgcgatgtct cgggcgaagg cgtgcagcaa gccctgctca agctggtgga
     1861 aggcacggtg gcgtcgattc cgccgcaagg cgggcgcaag caccccaacc aggaattcat
     1921 ccaggtggac accaccaata tcctgttcat ctgcggcggc gcatttgatg gtctggaaaa
     1981 gatcatccgc aaccgttcgc aaaagggtgg catcggcttt ggcgccgatg tgtactccaa
     2041 agatgacggg cgcgcggtgg gcaaggtctt gcaggaaacc gaaccggaag atctgatcaa
     2101 atttggcctg atccccgagt tcattggtcg cctgccggtc atcgccatgc tggacgagct
     2161 ggacgaggaa gcgctggtca ccatcctgac cgagccgaag aacgctttgg tgcgacagtt
     2221 ccagaagttg ttcgaaatgg aacaaaccga gttggaaatc cgaccatcgg ccttgcgcgc
     2281 catcgccaaa caggcattgg cccgcaagac tggcgcccgc ggtctgcgct cgattctgga
     2341 gcgcgcgctg ctggatacca tgtacgatct gccttccttg cagaatgtcg tgcaggtggt
     2401 ggtggacgaa accgtgatcg acaagggctc tgcgcctctg ctgattcacg ccgacgccga
     2461 gccggtggcg caatcggcct gatttcaggc agatgcccgc gcacgcaacc gctcgccagg
     2521 ccggcgagcg gtttttttat gccagccctc ggtgcatgac aacggcgaat cttgccggcg
     2581 ggcttgattt cacgcatgct tgccccatct agccttgcat tgccccgcta tttttacagg
     2641 tcgataccat gtctactacc gaattgctga ccgaggacac cagcctgccg cttctgccat
     2701 tgcgcgacgt ggtggtgttt ccgcatatgg tcatccccct gttcgtcggg cgccccaagt
     2761 ccatcaaggc gctggaaaag gccatggaat ccgacaagca gatcctgctg ctggcgcaaa
     2821 aatccgcctc caaggacgag ccctccgcag acgatctgta cgcgctgggc acggtggcca
     2881 ccgtgctgca actgctcaag ctgcctgatg gcacggtgaa agtgctggtg gaaggccgcc
     2941 agcgcgccag tgtgaatcac atcaatgaag acgccggcta ttttgtcggc gacatcaccc
     3001 cgctgccggc cgaaggcaag gaaaacaccg aaaccgaggc catgcgccgc gcgctgatcg
     3061 gtcagtttga gcagttcgtc aaactcaaca agaaaatccc gcctgaaatc ctgtcctcgc
     3121 tgtccgccat cgacaaggct ggccgtctgg ccgataccat cgccgcacaa ctgccgctga
     3181 aactggagca aaagcaggat gtgctggaaa tggccgacat caaaacccgg ctggagcacc
     3241 tgctggccca gctggaatca gaaatcgaca tcctgcaggt agaaaagcgc atccgtggcc
     3301 gggtcaaacg gcaaatggaa aaaagccagc gcgattacta cctgaatgag caggtgaaag
     3361 ccattcagcg cgaactgggc gaagaggatg aaagctctga cctggacgag ctggagaaaa
     3421 aaatcaaaac ggcgggcatg agcaaagaag cccgcgacaa ggccaatagc gaactgcgca
     3481 agctgcgcat gatgtcgccg atgtcggccg aagccaccgt ggtgcgcaac tacatcgaaa
     3541 cgctgatcga gctgccgtgg aagaaaaaaa ccaagatcag caaagacctg gaacacgctg
     3601 aaaccgtgct cgacgaagac cactttggcc tggacaaggt gaaagagcgc attgtcgagt
     3661 atctcgccgt gcagcagcgc gtggacaagc tcaaggcgcc gattctgtgc ctggtcggcc
     3721 cgcccggcgt gggcaagacc tcgctggggc agtcgattgc ccgcgcggtc aaccgtcagt
     3781 ttgtgcgcat ggcgctgggc ggcgtgcgcg acgaggccga gattcgcggc caccgccgca
     3841 cctatatcgg ctcgatgccg ggcaaggtgc tgcaaaacat gaccaaggtc ggggtgaaaa
     3901 acccgctgtt cctgctcgac gaagtggaca aaatgggtgc cgatttccgt ggcgatccct
     3961 cgtcggcgct actggaagtg cttgatccgg aacaaaatgc cacctttgtg gatcattacg
     4021 ccgaggtgga atacgacctg tccgacgtga tgtttgtcgc caccgccaac tcgatgaata
     4081 tcccgccggc gctgctggat cgcatggaaa tcatccggct gtccggctat accgaagacg
     4141 aaaaaatcgc catcgccgag cgctatctct tgcccaagca gatgaagaac accggcctga
     4201 gcgaagccga gctggcggtg caagagtccg ccgtgcgcga catcgtgcgc tattacaccc
     4261 gcgaagccgg cgtgcgcagc ctggaccgcg agctgaacaa gatctgccgc aaggtggtga
     4321 aggcgcaaca aggcaaaaaa gccgcagcca agccgacccg cgtcaccgtc aacgccaaga
     4381 atctggacaa atacctgggt gtgcgccgct acgactacgg tctggcggaa gaagccaatc
     4441 aggtcggcca ggtcaccggt ctggcgtgga ccgaagtggg cggcgaattg ctgaccatcg
     4501 aggcggtggg tttgccgggc aagggcacca tcatccgtac cggccagctg ggtgaagtga
     4561 tgcaggaatc catcaccgcc gccatgagcg tggtgcgccg ccgggcacgc agcctgggca
     4621 ttgccaaaga tttttacgaa aagaacgata tccacattca tctgcccgaa ggcgccacgc
     4681 ccaaggatgg tccgtcggcc ggtattggca ttgtcaccgc catcacctcg tcgctgaccg
     4741 gcattccggt gcgtgccgat gtggcgatga ccggcgaaat caccctgcgc ggcgaggtgt
     4801 tgccgattgg tggcctgaag gaaaaactgc tggcggccca gcgtggcggc atcaagcatg
     4861 tgctgattcc caagggcaat atcaaggatt tggcggaaat ccctgattcc atcaagaaaa
     4921 agctggaaat tcacccggtg aagtggattg aagaggtgtt tgccctggcg ctggagcaca
     4981 gcccgacccc gctggtggat gaggatgacg ttggcacgat tcctgccaaa gccgacgaac
     5041 tgcccgccgc tcgcccggtc acgcactgat tggccaattt gttgctaccc tgcccggtgt
     5101 gtttcttgcg ttgcagcatg agcacacccg ggcaggcagt cgcataaaaa tgcttccatc
     5161 cgtctggaat aagccggcaa agccgcttga cacaaggatt tgccgcttgc tataaagccg
     5221 gaagatttta atgaatgctg tgtgcgcaag ctcacaattc tcgtaaaggg gacaacagtg
     5281 aacaaatccg aactgatcga tgccatggcc gcgtcggctg acattcccaa ggctgccgct
     5341 ggcaaagccc tggacggcat ggtcgaagcg ctgacgcaag cgctgaagaa tggcgatacg
     5401 gtcacgctgg tgggctttgg cagcttctac gtgggcgaac gcgctgagcg caccggccgc
     5461 aatccgaaaa ccggtgaaac catcaccatc ccggcagcga aatctcctaa atttcgcgct
     5521 ggtaaaacgc tgaaagatgc gctataatct ttttctcggt tggttgaagc aatccagtta
     5581 gccgatctgg gcggttagtc tcagttggta gagcactgcc ttcacacggc aggggtcact
     5641 gg
//
LOCUS       DAOPYX010000203         5587 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_18176_length_5587_cov_10.663774, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000203 DAOPYX010000000
VERSION     DAOPYX010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 5587)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5587)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5587
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18176_length_5587_cov_10.663774"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..626
                     /locus_tag="PKN55_10295"
     CDS             <1..626
                     /locus_tag="PKN55_10295"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNX71678.1"
                     /translation="DCHARMDDTVGAVRQLADGVSQAAQRITELEATARQIGEIVDAI
                     GDIADQTNLLALNAAIEAARAGEAGRGFAVVADEVRKLSQATQQSAIEVNTLASDIRS
                     RVQHAAAGMSANLTHSETGARSLDGSDQALSAVAEHVGAMAGRSADIASETARQRDRA
                     AALDAQLAELERSAQANDQHARQLGDAARQVRDITATLHAQATRFRL"
     gene            686..1882
                     /locus_tag="PKN55_10300"
     CDS             686..1882
                     /locus_tag="PKN55_10300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX71679.1"
                     /translation="MKPVLPLLALGFGLAGAAQADEPVFSVSGYGTLAWTQMSGDNAQ
                     YRVGTAEPNGAGHAGSGETDSKLGVQIIARPTDKFSATLHLLSKKNVNNNFNPRVEWA
                     YGKYDLTPDVSLRAGRLALPGFVLSDYLNVGYSMTPVRGPVEVYAQLPISHFNGVDAL
                     WRTQAGPLALTVQPFAGRSNIHARFSDPQATRAEGDATVYGMNLVGQWDDWSLRIGRI
                     DAKVSLNAGPVDSLITTLRMMGENGAASRLEYQDKHSSFTGLGVGYDNGKLLAQGEYT
                     IRKAGGFLADTTGWYVMAGWRFGNVTPYVMTARQRVTSDTQYGGIANPMLSAYVNGLL
                     ASGNTSQKSWSLGARWDVAKNIAVKAQYDRIRPDHGSAAGLLISSDSKPLSSRHVATV
                     AMDFVF"
     gene            1893..2300
                     /locus_tag="PKN55_10305"
     CDS             1893..2300
                     /locus_tag="PKN55_10305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX71680.1"
                     /translation="MKTRIVCMLLASALSAAAGAETVVVVSAKSAVATLSKEQVADLF
                     VGKTQQLGGASAALVDQAELKEDFYSKATGRNAAQIKATWAKLLFTGKGNPPKEVSGN
                     AEVKKFVAGNPAAIGYIDKAAVDGSVKVVLSLP"
     gene            complement(2464..3570)
                     /gene="nemA"
                     /locus_tag="PKN55_10310"
     CDS             complement(2464..3570)
                     /gene="nemA"
                     /locus_tag="PKN55_10310"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012698092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
                     /protein_id="HNX71681.1"
                     /translation="MRADQLLSPVQIGGITLNNRIVMAPLTRLRAHEPGDVPNSALAA
                     EYYAQRADAGLLIAEATQISPVGKGYAGAPGIYNEAQVQAWQPITAAVHAKGGRMALQ
                     LWHVGRVSHTSLQPEHAAPVAPSAIAADANATVRNPDGQLGRAPCSLPRALETAEIAD
                     LIEDYRQATDRSRRAGFDLVEVHAAHGYLLHQFQSPLANQRTDQYGGSVENRARLTLE
                     VVDAVIGEWDSAHVGIRISPLGIFNGLDDTGQEDMAWYLADELAKRKLAYLHLSEPDW
                     AGAPALSDAFRHTLRARYPGKIIAAGGYTTEKAETLLKAKLIDAVAFGRPFIGNPDLV
                     SRLREDWPLVEFNPATVYAAGAEGYTDYPVYSPA"
     gene            complement(3703..4308)
                     /locus_tag="PKN55_10315"
     CDS             complement(3703..4308)
                     /locus_tag="PKN55_10315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018149764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNX71682.1"
                     /translation="MTAASPPHTDTREHILSTAEPLVLGKGFTALGLTELLSAAGVPK
                     GSFYHYFRSKEQFGQALLERYFANYDARLASHFAAEPGRMRDRLLSYFAGWITQACQA
                     DSHGSCLAVKLAGEVCDLSEPMREALAVGMAQVCARLADAIARAAAEGSLAACADPAA
                     LADALYAMWLGAALRTKVMRDSSPLVRALADTEQRLAPPSR"
     gene            complement(4305..4583)
                     /locus_tag="PKN55_10320"
     CDS             complement(4305..4583)
                     /locus_tag="PKN55_10320"
                     /inference="COORDINATES: protein motif:HMM:NF022592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2917 domain-containing protein"
                     /protein_id="HNX71683.1"
                     /translation="MRASGDFRVVVGAGELVRLDCDAPVRVLCEQGALWITSSAAAED
                     ICLSAGQQAQGCQGVVLLEGEGVLRVSLLDAPVPQACSRPTGLVDCAT"
     gene            4681..>5587
                     /locus_tag="PKN55_10325"
     CDS             4681..>5587
                     /locus_tag="PKN55_10325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008953177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HNX71684.1"
                     /translation="MNRLPPLAALRAFEAVARLGSVGKAADELHVTHGAVSQQLRVLE
                     DTLGVSLFLRHGKRLSVTEDGRMYALRLRMALADLAEATDDVLALPREDELVLAVLPS
                     FAAHWLVRRLPGLMRAQPALKLTLRASLEMVDFQVERVDAAIRMGAGGWEGLTQLPLF
                     TDELLAVAAPSFNGGVLPHTPQEILACPLIRSVEGWGHWLNAAGLPTQTLNGPMFTDS
                     NLVIEAVRQGQAITLTRRSLAHDLLQSGELVQLGELTAPYANPYWLVWPSRSQGKAKV
                     QVFAEWLEGEIADYLRAIGGALPSNR"
BASE COUNT          985 a         1871 c         1823 g          908 t
ORIGIN      
        1 ccgactgcca cgcgcgcatg gacgacaccg tcggcgcggt gcgccagctg gccgatggcg
       61 tcagccaggc cgcacagcgc atcaccgagc tggaggccac cgcccgccag attggcgaaa
      121 ttgtcgacgc catcggcgat attgccgacc agaccaatct gctggcgctg aacgccgcca
      181 tcgaagccgc ccgcgctggc gaggccgggc gcggttttgc cgtggtggcc gacgaggtgc
      241 gcaagctgtc gcaagccacc cagcaatccg ccatcgaggt caacaccctg gccagcgaca
      301 tccgcagccg cgtgcagcac gccgccgccg gcatgagcgc caacctcacc cacagcgaaa
      361 ctggcgcgcg cagcctggat ggctcggacc aggcgctgtc ggcggtggcc gagcatgtgg
      421 gggccatggc cgggcgcagc gccgacatcg ccagcgaaac cgcccgccag cgcgaccgcg
      481 ccgccgcgct ggacgcccag ctggccgagc tggagcgctc ggcgcaggcc aacgaccaac
      541 acgcccgcca actgggcgac gccgcccgcc aggtgcgcga catcaccgcc accctgcacg
      601 cccaggccac ccgtttccgt ttgtgaaccc gcctcaccct tgccgcgcca ggcggcttgc
      661 ctgggcgcgc tttttggatt cttgcatgaa acccgttctt ccgctgttgg cgctgggctt
      721 tggcctggcc ggcgccgccc aggccgacga gcctgttttt tctgtgtccg gttacggcac
      781 gctggcctgg acgcaaatgt ccggcgacaa cgcccagtac cgggtgggca cggctgagcc
      841 caatggcgcc ggccatgccg gcagcggcga aaccgacagc aagctgggcg tgcagatcat
      901 cgcccggccg acggataagt tttccgccac cttgcatctg ttgagcaaga aaaacgtcaa
      961 caacaacttc aacccgcgcg tggaatgggc ctacggcaaa tacgacctca ccccggatgt
     1021 gagcctgcgc gccggccggc tggcgctgcc gggttttgtg ctgtcggatt atctgaacgt
     1081 gggttactcg atgacgccgg tgcgcggccc ggtggaagtc tacgcccagc tgccgatttc
     1141 gcactttaac ggggtggacg cgctgtggcg cacccaggcc ggcccgctgg cgctgaccgt
     1201 gcagccgttt gccggccgct cgaacatcca cgcgcggttt tctgatccgc aagccacccg
     1261 cgccgagggc gacgccacgg tgtatggcat gaatctggtc gggcagtggg atgactggtc
     1321 gctgcgcatt ggccgcatcg acgccaaggt gtcgctgaac gccggcccgg tggacagcct
     1381 gataaccacg ctgcgcatga tgggcgaaaa cggcgcggcc agccggctgg aataccagga
     1441 caaacattct tcgttcaccg gcctgggcgt gggctacgac aacggcaagc tgttggcgca
     1501 gggcgaatac accatccgca aggccggcgg ctttttggcc gacaccaccg gctggtatgt
     1561 gatggccggc tggcgctttg gcaatgtcac cccctatgtg atgaccgccc gccagcgcgt
     1621 caccagcgac acgcaatatg gcggcatcgc caacccgatg ctgtccgcct atgtaaacgg
     1681 cctgctggcc tcgggcaaca ccagccagaa aagctggtcc ttgggtgcgc gctgggacgt
     1741 ggccaagaat atcgccgtca aagcccagta tgaccgcatc cgccccgacc acggcagcgc
     1801 cgctggcctg ttgatcagca gcgacagcaa gccgctgtcg tcccgtcatg tggccacggt
     1861 ggccatggac tttgtgttct gaggagccgc cgatgaaaac ccgcattgtc tgcatgctgc
     1921 tggccagcgc gctgagcgcc gccgccgggg cggaaaccgt ggtggtggtg tcggccaaat
     1981 cggccgtggc cacgctgagc aaggagcagg tggctgattt gtttgtcggc aaaacccagc
     2041 aactgggcgg cgccagcgcc gcgctggtgg atcaggccga gctgaaagaa gacttttaca
     2101 gcaaggccac cggccgcaac gccgcgcaga tcaaggccac ctgggccaag ctgttattca
     2161 ccggcaaggg caacccgccc aaggaagtca gcggcaacgc cgaggtgaag aagttcgtcg
     2221 ccggcaaccc ggcggcgatt ggctatatcg acaaggccgc agtggatggc agcgtgaaag
     2281 tggtgctgag cctgccgtaa ggtaagccag atcaaaaaca ccccctgcgg cagcgctgag
     2341 cgcaactggc cgcagggggt gtttttttgg ccaagcttac accgtaccga gtctctggct
     2401 cagcagatgc agaccgtgca acaagaccag aaggaggtag cctactgatc aagccgagcg
     2461 cgctcaggcc ggcgaataca ccggataatc ggtatagcct tccgcaccag cggcatacac
     2521 cgtggccggg ttgaactcca ccagcggcca gtcttcgcgc aggcggctga ccaagtccgg
     2581 gttgccgata aacggccggc caaaagccac ggcgtcgatc agcttggcct tgagcagggt
     2641 ttccgctttt tcggtggtgt agccgcctgc ggcgatgatc ttgcctgggt agcgcgcgcg
     2701 cagggtgtgg cggaaggcgt cggacaaggc cggcgcgccg gcccaatccg gctcggacag
     2761 gtgcagatag gccagcttgc gcttggccag ctcgtcggcc agataccagg ccatgtcttc
     2821 ctggccggtg tcgtccaggc cgttgaaaat gcccagcggc gaaatgcgga tgcccacatg
     2881 ggcgctgtcc cattcaccaa tcaccgcgtc caccacttcc agcgtcagcc gcgcgcggtt
     2941 ttccacgctg ccgccgtatt ggtcggtgcg ttgattggcc agcggcgact gaaactggtg
     3001 caaaagataa ccatgcgcgg cgtgcacttc caccagatca aagccggcgc ggcgcgagcg
     3061 gtcggtggcc tggcggtagt cttcgatcag gtcggcgatt tcggcggttt ccagcgcgcg
     3121 cggcagcgag cacggcgccc ggcccaattg gccatccgga ttgcgcaccg tggcgttggc
     3181 gtcggcggca atcgccgatg gggccaccgg ggcggcgtgc tccggctgca agctggtgtg
     3241 cgacacccgg cccacatgcc acagctgcaa ggccatgcgc ccgcccttgg cgtgcacggc
     3301 ggcggtaatc ggctgccagg cctgcacttg cgcttcgttg taaatccccg gcgcgccggc
     3361 atagcccttg cccacaggcg aaatctgcgt ggcctcggca atcagcaggc cggcgtcggc
     3421 gcgctgggcg tagtattccg ccgccagcgc gctattgggc acatcgcccg gctcgtgcgc
     3481 gcgcagccgg gtcagcggcg ccatgacaat acggttgttc agggtgatgc ccccgatctg
     3541 caccggggag agcagttggt ctgcacgcat ggaggcgtcc tttttgtggg tcaggcgttg
     3601 aaaacaacac accaatagac cagtcgtcta gtccgttggc aaatgcaagc cgcacctggg
     3661 tgcggcgggg taaagcaagc gtctttgctg ggtgagcggc gctcagcgcg atggcggcgc
     3721 cagccgctgt tcggtgtcag ccagcgcgcg caccagcggg ctgctgtcgc gcatcacctt
     3781 ggtgcgcaag gcggcgccca gccacatcgc gtacagcgca tcggccagcg ccgccgggtc
     3841 ggcacaggcg gccagcgagc cttctgccgc cgcgcgggcg atggcgtcgg ccagccgcgc
     3901 gcacacctgc gccatgccca ccgccagcgc ctcgcgcatc ggctcggaca aatcacacac
     3961 ctcgccggcc agcttcaccg ccaggcagct gccatggctg tcggcctgac aggcctgggt
     4021 gatccagccg gcaaaataac tcagcagccg gtcgcgcatg cgccccggct cggcggcaaa
     4081 atggctggcc agccgggcgt cgtagttggc gaagtagcgc tccagcaagg cctggccaaa
     4141 ttgctctttc gagcgaaaat aatggtaaaa cgaacccttg ggcacccccg ccgcgctcag
     4201 cagctcggtc aaacccagcg cagtaaagcc tttgcccagc accaggggct cggcagtgct
     4261 caggatatgt tcgcgggtgt cggtgtgcgg ggggctggcg gcagtcatgt ggcacagtct
     4321 actagaccag ttggtctaga gcaagcctga ggcactggcg catccagcag gctgaccctg
     4381 agcaccccct cgccttccag caacaccacg ccctgacaac cctgcgcctg ctggccggcg
     4441 ctcaggcaaa tatcctcggc cgccgcgctg ctggtgatcc acagcgcgcc ttgctcgcac
     4501 agcacgcgca ccggcgcatc acaatccagc cgcaccagct cgcccgcgcc caccaccacc
     4561 cgaaaatcac ccgacgctct catcgcacac cccaattgca tttaagattt gctaaattta
     4621 ccttaggata aacatcacaa aaatcgattt attttgggag ttttggtgag tatggctaac
     4681 atgaaccgtc tgccgccgct ggcggcgctg cgcgcttttg aggccgtggc gcggctgggc
     4741 agcgtgggca aggccgccga tgagctgcat gtcacccatg gcgcagtcag ccagcaattg
     4801 cgcgtgctgg aagacaccct gggcgtaagc ctgtttctgc gccatggcaa gcgcttgtcg
     4861 gtgacagaag acggccggat gtacgccttg cggctgcgca tggcgctggc cgatctggcc
     4921 gaggccaccg acgatgtgct ggcgctgccg cgcgaggacg aactggtgct ggccgtgttg
     4981 ccctcgtttg ctgcgcactg gctggtgcgc cgcctgcccg gcctgatgcg cgcccagccg
     5041 gcgctgaagc tgaccctgcg cgccagcctg gagatggtgg attttcaggt ggagcgggtg
     5101 gacgccgcca tccgcatggg cgcaggcggc tgggaagggc tcacccagct gccgctgttc
     5161 accgacgagc tgctggcagt ggcggcgccc agctttaacg gcggcgtgtt gccgcacacg
     5221 ccgcaagaga ttctggcctg cccgctgatc cgctcggtgg aaggctgggg ccactggctg
     5281 aacgccgccg gcctgcccac ccagaccttg aatgggccga tgttcaccga ctccaatctg
     5341 gtgatcgaag ccgtgcgcca aggccaggcc atcaccctga cccgccgctc actggcgcac
     5401 gacctgctgc aaagcggcga actggtgcaa ctgggcgagc tgaccgcccc ctacgccaac
     5461 ccctactggc tggtgtggcc cagccgcagc cagggcaaag ccaaggtgca ggtgtttgcg
     5521 gaatggttgg aaggcgaaat cgccgattat ttgcgtgcga ttggcggcgc tctgccatcc
     5581 aaccgct
//
LOCUS       DAOPYX010000204         5548 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_18380_length_5548_cov_8.006190, whole genome shotgun sequence.
ACCESSION   DAOPYX010000204 DAOPYX010000000
VERSION     DAOPYX010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 5548)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5548)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5548
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18380_length_5548_cov_8.006190"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..297)
                     /locus_tag="PKN55_10330"
     CDS             complement(<1..297)
                     /locus_tag="PKN55_10330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012697504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HNX71685.1"
                     /translation="MSESANDKVRLDKWLWAARFYKTRGLAQDAIEAGHVRLNGERPK
                     PARTVKVGDRLELRLNQLEYHLEVRGLSEKRGPAPVARELYFESEDSMARREARQ"
     gene            complement(297..947)
                     /locus_tag="PKN55_10335"
     CDS             complement(297..947)
                     /locus_tag="PKN55_10335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003774657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HNX71686.1"
                     /translation="MATVSLAGRDVTVSNIFCIGRNYAAHVAELGNRPEAEPLVFLKP
                     TSALLADGGQIELPVYSQDVHHETELVALIGRDARHVAEADALDYVAGYGIGLDLTAR
                     DTQSELKAKGMPWTKAKAFATAACVSAFAPASAVANPQALTFSLTVNGERRQQGDTAL
                     MLFPLAQLISFLSQHYGLTAGDLIYTGTPEGVARLQSGDVLALGLDGLAQAQFTVA"
     gene            complement(1050..2183)
                     /locus_tag="PKN55_10340"
     CDS             complement(1050..2183)
                     /locus_tag="PKN55_10340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014086545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14-type cytosolic carboxypeptidase"
                     /protein_id="HNX71687.1"
                     /translation="MKISSTFDAGSIDVVALDSFDDIRLRLRPDNAAEFAQWFYFRLQ
                     GAAYQPCTMRILNAGEAAYPEGWVNYQPCASYDRVNWFRVPARYEDGVLIIEHTPLAS
                     SIYYAYFEPYSYEQHLRLIGDAQSSGLVQLSDLGSTLDGRDLNLLTIGHEVSSDLKVW
                     IIARQHPGETMAEWFIEGLLGRLLDTHDPLARALMDKATFYVVPNMNPDGSVRGNLRT
                     NAAGANLNREWLEPSAERSPEVLWVREKMQETGVDLFLDIHGDESLPYIFVAGTEGVP
                     SYSPRVAALETTFKQHFLLASPDFQDEHGYAKDAPGQANLSMATAWVGNHFECLAFTL
                     EMPFKDNANLPDDDMGWNGQRSLRLGEAILQPVYAVLNELREA"
     gene            complement(2292..2849)
                     /gene="phaP"
                     /locus_tag="PKN55_10345"
     CDS             complement(2292..2849)
                     /gene="phaP"
                     /locus_tag="PKN55_10345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017509325.1"
                     /note="Members of this family are phasins (small proteins
                     associated with inclusions such as PHA granules). Note
                     that several different families of phasins have been named
                     PhaP despite very little sequence similarity to each
                     other; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01841 family phasin"
                     /protein_id="HNX71688.1"
                     /translation="MVNPQAQFSQAALHGVDTAMRLTQIGLNSAERLFKLQFQAGRAL
                     LEEHVRNLHSLTEGAPQELPARINTLAAQTLEQMVNHSRNTYEVLSATQNELTQLVED
                     QFSQLNRTVISSIDVLAKNAPAGSDAAVNALKSSLAASAAAVNSITKAAHQVAEFTET
                     SVKAATAATADAVKTAANKASTPAA"
     gene            3413..3982
                     /gene="phaR"
                     /locus_tag="PKN55_10350"
     CDS             3413..3982
                     /gene="phaR"
                     /locus_tag="PKN55_10350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate synthesis repressor PhaR"
                     /protein_id="HNX71689.1"
                     /translation="MSGEKRVIKKYPNRRLYDTATSSYITLGDVKQLVLDHVDLQVVD
                     AKTQDDITRSVLLQIILEEEAGGMPMFTYDVLTQIIRFYGNTMQGLMGNYLEKNLELF
                     SQMQQKLQDQTRQMVGDNPMFGNHNMWSEYMKFQAPAIQNMMTSYLEQSTNMFVEMQN
                     QMQTRTKNLFSGFPFQPYTPPSSDKDPKN"
     gene            4122..5450
                     /gene="rimO"
                     /locus_tag="PKN55_10355"
     CDS             4122..5450
                     /gene="rimO"
                     /locus_tag="PKN55_10355"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014086546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HNX71690.1"
                     /translation="MSHTVPKVGFVSLGCPKALVDSEHILTRLRAEGYEIAPSYDGAD
                     LVVVNTCGFIDSAVAESLDAIGEALNENGKVIVTGCLGARDDGKMVTDMHPSVLAVTG
                     PHAASEVMDHIHQHLPKPHDPFMDLVPAAGVKLTPRHYAYLKISEGCNHRCTFCIIPG
                     LRGDLDSRPVHEVLKEAEALVKGGAKELLVVSQDTSAYGVDIKYRTGFWQGRPVRTRM
                     TELCEELGKLGAWVRLHYVYPYPHVDEVIPLMAEGKILPYLDIPFQHASQKVLKAMKR
                     PANADNVLQRIRKWREICPELVIRSTFIVGFPGETEEDFNILLDFLREAELDRVGCFT
                     YSPVDGAAANALPDPVPEEVKEDRKARLMELQAEISARRLATRVGQTMTVLVDEIDEE
                     GAVCRSYADAPEIDGLVFVADSDGLQVGDWVTVRIVESDEYDLWGERLEA"
BASE COUNT         1143 a         1680 c         1685 g         1040 t
ORIGIN      
        1 ctggcgcgcc tcacggcggg ccatgctgtc ttcgctttca aagtacaact cgcgcgccac
       61 cggcgccggg ccgcgttttt ccgacagccc gcgcacctca agatggtatt ccagctgatt
      121 gagccgcaac tccagccggt cgcccacttt caccgtgcgc gccggtttcg ggcgctcgcc
      181 attcagccgc acatggccag cctcgatggc atcctgcgcc aggccgcgcg ttttataaaa
      241 gcgcgccgcc cacagccatt tgtccagccg caccttgtcg ttggccgatt cgctcatcac
      301 gccaccgtaa actgggcctg agccagacca tccagaccca gcgccagcac atcgcccgat
      361 tgcaagcgcg ccacgccttc cggggtgccg gtgtaaatca ggtcaccagc ggtcaggccg
      421 tagtgctgcg acaaaaagct gatcagctgc gccagcggaa acagcatcaa ggcggtgtcg
      481 ccctgttggc ggcgctcgcc atttaccgtc aggctgaagg tcagcgcctg cggattggcc
      541 accgcgctgg ccggggcaaa tgcgctgacg caggcggcgg tggcaaaggc cttggccttg
      601 gtccacggca tgcccttggc cttgagctcg ctttgcgtgt cgcgcgcggt aagatccaaa
      661 ccaatgccat agccggccac atagtccagc gcatcagctt cggccacatg gcgggcatcg
      721 cggccaatca gcgccaccag ctcggtttca tggtgcacat cctgcgaata caccggcagc
      781 tcaatctgcc cgccatcggc cagcagcgcc gacgtcggct tgagaaacac cagcggctcg
      841 gcttccgggc ggttgcccag ctcggcgacg tgggcggcgt agttgcggcc aatgcagaaa
      901 atattgctga ccgtcacatc acggccggca agcgaaacgg ttgccatggc aagtccttgc
      961 agggaaagaa aaaaccaaca agccggcaag ccggcatgtc tgggatgatg gcagccgggc
     1021 gggtgttaac gcccagcccg gcacacgctt tacgcctcgc gcagctcgtt cagcacggca
     1081 tacaccggct gcaggatggc ttcgcccagg cgcaggctgc gctggccgtt ccagcccatg
     1141 tcgtcgtccg gcagattggc gttgtccttg aacggcattt ccagggtgaa ggccaggcat
     1201 tcaaagtgat tgcccaccca ggcggtggcc atgctcagat tggcttggcc aggcgcatcc
     1261 ttggcgtagc cgtgttcatc ctgaaaatcc gggctggcca gcaggaagtg ctgcttgaag
     1321 gtggtttcca gcgccgccac gcgcgggctg tagctgggca cgccctcagt gccggcgaca
     1381 aagatataag gcaggctttc gtcgccgtgg atatccagga acaaatccac cccggtttcc
     1441 tgcatttttt cccgcaccca cagcacttcc gggctgcgct cggcgctggg ctccagccat
     1501 tcgcggttga ggttggcgcc ggcggcattg gtgcgcagat tgccgcgcac cgagccatcc
     1561 gggttcatat tgggcaccac ataaaaagtg gctttatcca tcagtgcacg tgccagcgga
     1621 tcgtgggtgt ccagcaggcg gcccaagagg ccttcgataa accattcagc catggtttcg
     1681 ccagggtgct ggcgggcgat gatccagact ttcaggtccg agctgacttc atggccgatg
     1741 gtcagcagat tgaggtcgcg cccgtccagg gtcgagccca ggtcggacaa ttgcaccagg
     1801 cccgagcttt gtgcgtcacc aatcaggcgc agatgttgct cgtaggaata cggctcgaag
     1861 taagcgtaat aaatgctgct ggccagcggg gtgtgttcga tgatcagcac gccatcttca
     1921 tagcgggcgg gaacccggaa ccagttgacg cggtcatagg aagcacaggg ctggtaattt
     1981 acccagcctt ccgggtaggc cgcttcaccg gcgttgagga tgcgcatggt gcagggctgg
     2041 taggcggcac cctgcaaacg gaagtaaaac cactgggcaa actcggcggc gttgtccgga
     2101 cgcagacgca gacggatgtc gtcaaagctg tccagggcca cgacgtcgat gctgccggcg
     2161 tcaaaggtgc tgctgatttt catgtggcgg tgtccacggg tcggaaaagt cgattgtagc
     2221 gccaaatggg cgtaaaaaaa ccccgcgaca actggcgcgg ggtttggata tagcgtgtgg
     2281 agccgggtga atcaggctgc cggggtagaa gccttgtttg cggcggtctt cacggcatca
     2341 gcggtggcag cagtggcggc cttgacgctg gtttcggtga actcggccac ttggtgggca
     2401 gctttggtga tgctgttcac ggcggcggca gaggcggcca gcgaggattt cagcgcgttg
     2461 acagcggcgt cagagccagc cggggcattc ttggccagca cgtcgatcga gctgatcacg
     2521 gtgcggttga gctggctgaa ctgatcttcc accagttggg tgagttcgtt ctgggtggcc
     2581 gacagcactt cgtaggtgtt gcgcgagtga ttgaccattt gctccagcgt ttgcgcagcc
     2641 agggtgttga tgcgtgccgg cagttcctgc ggggcgcctt cggtcaggct gtgcaggttg
     2701 cgcacatgtt cttccagcag ggcgcgacca gcctggaatt gcagcttgaa caagcgttcg
     2761 gcgctgttca ggccgatttg agtcaagcgc atggcggtgt caacaccatg cagggcggcc
     2821 tggctgaatt gggcttgcgg gttcaccatg tcaatctcct cgtagacaat tgtttgataa
     2881 ggggaatttg gtgtgtgctt cccctcagac gattgcattc taggaagcat tgtgcgacgc
     2941 gtcaagcact attttgtgcg tcgcacaaaa tttttcttca gtaaaaataa taccttgtaa
     3001 cacggtggca aaagatgcag acaagtcatg cggctatggt aaagttctct tttgcactgc
     3061 aatacggtct gcggcaactt ggcagcagag gacgcatccg gccatcatgc cgtgcgccac
     3121 ccgcagcatc gcagacctgt ccgatgcatg gcccgcatgc atatggcaag cggtggatgc
     3181 aggcctgaca aggcctgtgc ggccaatatc gtgcggacga cgcttgccac agaccgggca
     3241 aaatttggca tcatgccagc cggagtgcgc cttcagccga caaggggaga gccagacaat
     3301 ggccggcgag cgcaaggtgc agtcctgccc ctcgacatcc actgcgatac gcgcgctgtt
     3361 gcaagttcgc cacggtgcgc aatgacatac caaaaaaaac cggaggctcg acatgagcgg
     3421 agagaagcgc gttatcaaga aatacccgaa ccgtcgtctg tacgacacgg ccaccagttc
     3481 ctacatcacc ctgggcgacg tcaagcagct ggtgctggat cacgttgacc tgcaagtggt
     3541 ggacgccaaa acccaggacg acatcacccg cagcgtgctg ctgcaaatca tcctggaaga
     3601 agaagccggc ggcatgccga tgttcaccta cgatgtactg acccagatca tccgcttcta
     3661 cggcaacacc atgcagggcc tgatgggcaa ctatctggaa aagaacctcg aactcttctc
     3721 gcagatgcag caaaaactgc aagaccagac tcgccagatg gtgggcgaca acccgatgtt
     3781 cggcaaccac aatatgtgga gcgaatacat gaagttccag gccccggcca tccagaacat
     3841 gatgaccagc tacctggagc aaagcaccaa tatgttcgtg gaaatgcaga accagatgca
     3901 gacccgcacc aaaaacctgt tcagcggctt tcccttccag ccctacaccc cgccctccag
     3961 cgacaaagat ccgaaaaact gatctgccag cgggcacggg ctgcccgccg ccgccatgag
     4021 catgcgcctt tcgcgccgcc atggctgttg tatacttgtg ggcatgatcc agccgggcat
     4081 caccgatgtc cggctttatt tgttttcacg agtattcccc catgtcccat accgtaccca
     4141 aagtcggctt tgtcagctta ggctgtccca aggcgctggt tgactctgaa cacatcctca
     4201 cccgactgcg cgccgaaggc tatgaaatcg cccccagcta cgatggcgcc gacctggtgg
     4261 tggtcaacac ctgtggcttt attgattctg ccgtggccga atcgctggac gccatcggcg
     4321 aggcgctgaa cgaaaacggc aaggtgatcg tcaccggctg tctgggcgcg cgcgacgacg
     4381 gcaagatggt caccgacatg cacccctcgg tgctggccgt gaccggccca cacgccgcca
     4441 gcgaggtgat ggaccacatc caccaacacc tgcccaagcc gcacgatccg ttcatggacc
     4501 tggtgccggc cgctggcgtc aagctcaccc cgcgccacta tgcctatctg aagatttctg
     4561 aaggttgtaa tcaccgctgc accttctgca tcatccccgg tctgcgcggc gacctggaca
     4621 gccggccggt gcatgaagtg ctgaaagaag ccgaagcgct ggtcaagggc ggcgccaaag
     4681 agctgctggt ggtgtcgcaa gacaccagcg cctacggcgt ggacatcaaa taccgcaccg
     4741 gcttctggca aggccgcccg gtgcgcacac gcatgaccga gctgtgcgaa gagctgggca
     4801 agctgggcgc ctgggtgcgc ctgcactacg tctaccctta cccgcatgtg gatgaagtca
     4861 tcccgctgat ggccgagggc aaaatcctgc cgtatctgga tatccccttc cagcacgctt
     4921 cgcaaaaagt gctgaaagcg atgaaacgcc cggccaacgc cgacaatgtg ctgcaacgca
     4981 tccgcaaatg gcgggaaatc tgcccggagc tggtgatccg cagcaccttt atcgttggct
     5041 ttcccggtga aaccgaagaa gacttcaata tcctgctgga ctttctgcgc gaagccgagc
     5101 tggaccgagt gggctgtttc acctattcgc cggtggacgg cgccgccgcc aacgcgctgc
     5161 ccgacccggt gccagaagaa gtaaaagaag accgcaaggc gcgcttgatg gagctgcaag
     5221 ccgaaatcag cgcccgccgc ctggccaccc gcgtcgggca aaccatgacc gtgctggtgg
     5281 acgagatcga cgaggaaggc gcggtatgcc gcagctacgc cgacgcgccg gaaatcgacg
     5341 gcctggtgtt tgtcgccgac agcgatggcc tgcaagtggg cgattgggtg actgtgcgca
     5401 ttgttgaaag cgatgaatac gatctgtggg gcgagcgact ggaagcgtaa gcgcacacac
     5461 taatttagcg ccgctcagca cactcagcga agccttcctg gctaggcacg cgaccgcaga
     5521 cagtacgagc agtacggcaa ggtcgcgt
//
LOCUS       DAOPYX010000205         5532 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_18446_length_5532_cov_10.989410, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000205 DAOPYX010000000
VERSION     DAOPYX010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 5532)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5532)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5532
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18446_length_5532_cov_10.989410"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            111..596
                     /gene="ybaK"
                     /locus_tag="PKN55_10360"
     CDS             111..596
                     /gene="ybaK"
                     /locus_tag="PKN55_10360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019924593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HNX71691.1"
                     /translation="MSQKPPVTQAIRMLRAHHVAWTDHLYAYEEKGGTRVSARELGVD
                     EHAVIKTLIMHDEQKRPLVVLMHGDCEVSTKNLARQLAVKSIEPCPPAIADKHSGYQV
                     GGTSPFGTRQAMPVYMEASIAELPRIYLNGGKRGYLIGVDPADVVRVLQPTLVNVAVV
                     G"
     gene            complement(709..1545)
                     /gene="nifM"
                     /locus_tag="PKN55_10365"
     CDS             complement(709..1545)
                     /gene="nifM"
                     /locus_tag="PKN55_10365"
                     /inference="COORDINATES: protein motif:HMM:TIGR02933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen fixation protein NifM"
                     /protein_id="HNX71692.1"
                     /translation="MPAYLELKLSWELFQRAPQALNAEERAKLDKIARRQQQLEAAIL
                     SSREAEDVRVGDAELHARLDTLAARYASPEELTTELNAVGLNQASLAEAVLRDLRVEA
                     LLEHVASKTELASDEDAERYYREHPQAFSRPETRTMRHILVTFNNSREQVKALALLDK
                     VRRTLVDEDSFAKAALRYSHCPTAMQGGALGTLKRGQLFPELEPSAFGMDAGEVSDPI
                     ESPIGLHILRCDAIQRGETVSFTQAREKILSHMNDKRREAAQKAWIVCQLARTSSAQT
                     IG"
     gene            complement(1529..2032)
                     /locus_tag="PKN55_10370"
     CDS             complement(1529..2032)
                     /locus_tag="PKN55_10370"
                     /inference="COORDINATES: protein motif:HMM:NF016226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen fixation protein NifZ"
                     /protein_id="HNX71693.1"
                     /translation="MMNARWEYGDGVRLVRNVKNDGTYPGMAVGAPLVRRGSIGHVVD
                     IGTFLQDQVIYSVHFLEDGRIVGCREEELIDINDPWTPSRFEFRDKVCPRMPLTLGGE
                     TLTPAGAVGEVLRVIRDAPGGVAYHLHFAEQLPGRVFQVAETLLDPAAGWIPAGEEEV
                     EHDASLS"
     gene            complement(2029..2370)
                     /locus_tag="PKN55_10375"
     CDS             complement(2029..2370)
                     /locus_tag="PKN55_10375"
                     /inference="COORDINATES: protein motif:HMM:NF015182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogenase-stabilizing/protective protein NifW"
                     /protein_id="HNX71694.1"
                     /translation="MDTDLSLLEALDELSSAEDFLDYFSVPYTPSVVQVNRLHILQRF
                     HDYLGRYAANMPSEESAQRSIYRLWLERAYLDFVESDALTEKVFRVFQQHDLPGGGHT
                     SFVSLDRVFSS"
     gene            complement(2382..2888)
                     /locus_tag="PKN55_10380"
     CDS             complement(2382..2888)
                     /locus_tag="PKN55_10380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX71695.1"
                     /translation="MDLLDFTDCALYFEAELSPEVEQLMHRAAECYGEPEAELALLRA
                     FLHAPDSLTVLVGLYRYYFYQHRLAEALDVAYRAMRLASARLGISDDWRRLDEHGLAV
                     AAAQSFGLLRFYLLALKAAGVVLLRLGDIDNARDHLSKLAALDQRDQLGASALLDIID
                     PVPDVSAA"
     gene            complement(2891..3706)
                     /gene="cysE"
                     /locus_tag="PKN55_10385"
     CDS             complement(2891..3706)
                     /gene="cysE"
                     /locus_tag="PKN55_10385"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012536566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HNX71696.1"
                     /translation="MSDACTLSPPMTDDASRPLGLAAQLREDLHCVFQRDPAARTRFE
                     VLTTYPGVHAILGHRLAHRLWTRGWRYPARLLSFAVRLFTNVDIHPGAVIGRRFFIDH
                     GACVVIGETAEVGDDVTLYHGVTLGGTSWKQGKRHPTLSDGVVVGAGAKILGPIVVGP
                     RVRVGANSVVIKDVPEDATVVGVPGRVVTSRRQGQEDVQARLDHHLLPDPVARSISCL
                     VDRIDQLERELALLRGQTPPHSDDGHCRTCSAGDLCCEQVDAAAPQVIEVTKA"
     gene            complement(3699..4838)
                     /gene="nifV"
                     /locus_tag="PKN55_10390"
     CDS             complement(3699..4838)
                     /gene="nifV"
                     /locus_tag="PKN55_10390"
                     /EC_number="2.3.3.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR02660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004410 - homocitrate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homocitrate synthase"
                     /protein_id="HNX71697.1"
                     /translation="MQSPSWVTINDTTLRDGEQSAGVAFSLSEKLSIARQLVAIGVPE
                     LEIGIPAMGEEERDSIRAVAALGLPARLMVWSRMHPDDIDACADLGAHLIDVSIPASD
                     QHLAYKLRQNRDWLLRTLPDTLARAGDLGMEVCVGCEDASRADLGFLCQLAEVAAKAG
                     ARRLRFADTLGILEPFTTYERIAALRAASGLEIEIHAHDDLGLATANTLAACRGGATH
                     ANTTVNGLGERAGNAPLEEVVLGLAKLHGIATGVDLKHFLALSQQVATASGRTVSWQK
                     SLVGQGVFTHEAGIHVDGLLKHPDNYQGFDPSEVGRSHRIVLGKHSGPRAVMTVYAGL
                     GVHLDMAMAERLLARVRAFVLARKRPPENPELLNLLSDEEMARHV"
     gene            5067..5531
                     /locus_tag="PKN55_10395"
     CDS             5067..5531
                     /locus_tag="PKN55_10395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011133765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HNX71698.1"
                     /translation="MADTPPLSTTGNPALKLDAQLCFALYSTLLGVNKVYRTLLRELD
                     LTYPQYLVMLVLWERDERNVSDICSQLYLETATLTPLLKRLEARGLVNRRRSPSDERQ
                     VIVSLSEAGRALYAQVQHIPGCMSEALSCSQDDIVALRKQLLTLRDALFQHV"
BASE COUNT         1015 a         1836 c         1723 g          958 t
ORIGIN      
        1 gtcagcctga acgcgggcgg gaggccactc ccgcccgcgt ttgcatctgg ccccctgtct
       61 gccggcagcg ctacactgcg ggttttcttc ctctgctgcg gtttttcacc atgagccaga
      121 aaccccctgt cacccaggcc atccgcatgc tgcgcgccca ccatgtggcg tggacggatc
      181 acctgtacgc ctacgaagaa aaaggcggca cccgcgtctc ggcccgcgaa ctgggcgtgg
      241 acgaacacgc cgtgatcaaa accctgatca tgcacgacga acaaaaacgc ccgttggtgg
      301 tgctgatgca cggcgattgc gaagtctcca ccaagaacct ggcccgccaa ttggcggtaa
      361 aaagcatcga gccctgcccg ccggcgattg ccgacaaaca ctccggctat caagtgggcg
      421 gcaccagccc atttggcacg cgccaggcca tgccggtgta tatggaagcc agcatcgccg
      481 agctgccgcg catctatctg aatggcggca aacgcggcta tttgattggg gttgatccgg
      541 ctgacgtggt gcgggtattg cagcccacgc tggtgaatgt ggcggtggtg ggctgagaaa
      601 ccgcctgcgc gcatcactac gcccgacaca gtctgacctc gtactgcgta cataaaaaaa
      661 ccgggtaaac ggcatagccg cacccggttt tttgtttcat caagtctctt agccgatggt
      721 ctgggcgctg ctcgtgcgcg ccaattgaca gacaatccag gctttttgcg ccgcttcgcg
      781 gcgtttgtcg ttcatatggc tgaggatttt ctcccgcgcc tgagtaaagc tcaccgtctc
      841 gccgcgctgg atggcgtcgc agcgcagaat gtgcaggcca atcggcgatt cgatcgggtc
      901 ggacacttcg ccggcatcca tgccaaacgc cgagggctcc agttcgggaa acagctggcc
      961 gcgcttgagc gtgcccagcg cgccgccctg catggcggtg gggcagtgcg aatagcgcag
     1021 cgccgccttg gcgaagctgt cttcgtccac cagggtgcgg cgcaccttgt ccaagagcgc
     1081 caaggccttg acctgctcgc gcgagttatt gaaggtcacc agaatatggc gcatggtgcg
     1141 ggtttccggc cggctgaacg cctgcggatg ctcgcggtag taacgctcgg cgtcttcgtc
     1201 gctggccagt tcggttttgc tggccacatg ctccagcagc gcctccaccc gcaaatcgcg
     1261 caacaccgcc tcggccaggc tggcttggtt cagcccgacg gcgttcaact cggtggtcaa
     1321 ttcttcgggg ctggcgtaac gcgcggcgag cgtgtccagc cgcgcgtgca gctcggcgtc
     1381 gcccacccgc acgtcttccg cttcgcggct ggataaaatc gccgcttcca gctgttgctg
     1441 gcggcgggcg attttgtcca gtttggcgcg ttcttcggcg ttcagcgcct gcggcgcgcg
     1501 ctggaacagc tcccaggaga gtttcagttc aagataggct ggcatcgtgc tccacctctt
     1561 cttctccggc cggaatccag ccggcggctg ggtccagcag ggtttccgcc acctgaaaca
     1621 cccggccggg cagttgctcg gcaaagtgca ggtgataggc cacgccgccc ggcgcatcgc
     1681 ggatcacccg cagcacctcg cccacggcgc cggccggggt cagcgtttcg ccgcccaggg
     1741 tcagcggcat gcgcggacag actttgtcgc gaaactcaaa gcgcgagggc gtccatgggt
     1801 cgttgatgtc gatcagctct tcttcgcgac agccgacaat gcggccgtct tccagaaaat
     1861 gcaccgaata aatcacctga tcctgcaaaa aggtgccgat atcgaccaca tggccaatgc
     1921 tgccgcgccg caccagcggc gcgcccaccg ccattccggg gtaggtgccg tcgtttttga
     1981 cgttgcgcac caagcggacc ccgtcgccgt actcccagcg cgcgttcatc atgacgagaa
     2041 cacccgatcc agcgacacaa acgaggtgtg gccgccgcct ggcaggtcat gctgctggaa
     2101 cacgcgaaac actttttcgg tcagcgcgtc ggattcgaca aagtccaggt aggcgcgctc
     2161 cagccacagc cggtagatgc tgcgctgggc ggattcttct gacggcatat tggcggcgta
     2221 gcggcccagg tagtcgtgaa agcgctgcag gatgtgcagg cggttcacct gcaccaccga
     2281 cggggtgtag ggcacgctga agtaatccag aaaatcctcg gcgctggaga gttcgtccag
     2341 tgcttccagt aaagacagat cggtatccat ggggctctcc ttcaggcagc ggaaacgtcg
     2401 ggaacggggt cgatgatgtc cagcaaggcg ctggcgccaa gttggtcgcg ctgatccagc
     2461 gcggccagtt tgctcaagtg gtcgcgggcg ttgtcgatgt cgcccagccg cagcagcacc
     2521 acgccggcgg ctttcaacgc cagcaaataa aaacgcagca aaccaaacga ttgcgcggcg
     2581 gccaccgcca ggccgtgctc gtccagccgc cgccagtcgt ccgatatgcc cagccgcgcg
     2641 ctggccaggc gcatggcgcg gtaagccacg tccagcgctt cggccaggcg gtgctgataa
     2701 aagtaatagc ggtacagccc caccagcacg gtcaggctgt ccggggcgtg caaaaaggcg
     2761 cgcagcaggg ccagctcggc ttcgggttcg ccgtagcact ctgcggcgcg atgcatcagc
     2821 tgttccactt cgggcgagag ctccgcctcg aagtacagcg cacagtcggt aaaatccagc
     2881 aagtccatgg ttaggccttt gtcacttcaa tgacctgcgg cgcggcggca tccacctgct
     2941 cgcagcacag gtcgccggcc gaacaggtgc gacagtggcc gtcgtcagaa tgcggcggcg
     3001 tttgcccacg caatagcgcc agctcacgct ccagttggtc aatgcgatcc accaggcagc
     3061 tgatcgagcg cgccaccggg tcgggcagca ggtgatgatc cagccgcgcc tgcacatctt
     3121 cctggccctg gcggcgactg gtcaccaccc gaccgggcac gcccaccacg gtggcgtctt
     3181 ccggcacatc cttgatcacc accgaattgg cccccacccg cacgcgcggg ccaaccacaa
     3241 tcggcccgag aatcttggcg ccagcgccca ccaccacgcc atccgacaag gtggggtggc
     3301 gcttgccctg cttccaggag gtgccgccca gcgtcacgcc gtgatacagc gtcacgtcgt
     3361 cgcccacctc ggcggtttcg ccaatcacca cacaggcgcc gtggtcgata aaaaagcggc
     3421 ggccaatcac cgcgccgggg tggatgtcca cattggtgaa cagccgcacg gcaaagctca
     3481 acagccgcgc cgggtagcgc cagccgcgcg tccacagccg gtgggccagg cggtggccca
     3541 gaatggcgtg cacgccgggg taggtggtca gcacctcaaa acgggtgcgc gcggccgggt
     3601 cgcgctgaaa cacgcagtgc aaatcctcgc gcagctgcgc ggccaggccc agcgggcggg
     3661 aagcgtcatc agtcatcggc ggactcaggg tgcaggcatc agacatggcg ggccatctcc
     3721 tcatcgctca gcaaattcaa cagttcgggg ttttccggcg ggcgcttgcg cgccagcaca
     3781 aaggcgcgca cccgcgccag caggcgctcg gccatcgcca tatccagatg cacgcccaaa
     3841 ccggcgtaca ccgtcatcac cgcgcgcggg ccagaatgct tgcccagcac aatgcggtgg
     3901 ctgcggccca cctcgctggg gtcaaaaccc tggtagttgt ccgggtgttt cagcaggcca
     3961 tcgacatgga tgcccgcctc atgggtgaac accccttgcc cgaccaggct tttttgccag
     4021 gacaccgtgc gcccggacgc cgtcgccacc tgctgcgaca gcgccagaaa atgctttaaa
     4081 tccaccccgg tggcgatgcc gtgcagcttg gccagcccca gcaccacttc ttccagcggc
     4141 gcattgccgg cgcgctcgcc caggccgttc accgtggtgt tggcgtgggt ggcgccgccg
     4201 cgacaggcgg ccagggtgtt ggcggtggcc aggcccaggt cgtcgtgggc gtggatttca
     4261 atctccagcc cgctggccgc gcgcaaggcg gcaatccgct cgtaggtggt gaacggctcc
     4321 agaatgccca gcgtgtcggc aaagcgcagc cggcgcgcgc cggccttggc cgccacctcg
     4381 gccagctggc acaaaaaccc caggtcagcg cgcgaggcgt cttcacagcc cacgcacacc
     4441 tccatgccca gatcgcccgc gcgcgccagc gtgtccggca aggtgcgcag cagccagtcg
     4501 cggttctgac gcaatttgta ggccaggtgt tggtccgacg ccggaatcga cacatcaatc
     4561 agatgcgcgc ccaaatcggc gcaggcgtcg atgtcgtccg ggtgcatgcg gctccacacc
     4621 atcagccgcg ccggcaggcc cagcgccgcc accgcgcgga tgctgtcgcg ctcttcctcg
     4681 cccatcgccg ggatgccgat ttccagctct ggcacgccga tggcgaccaa ctggcgggca
     4741 atgctcaatt tttcgctcag cgaaaacgcc accccggcgg actgctcgcc atcgcgcaag
     4801 gtggtgtcgt tgatggtgac ccaagacggg gattgcatgg ctggcctcct ggaaaccaag
     4861 ggcgacgccg cgcgccgccg gattgaatcc tggattagca agtccggggc cagaagaaaa
     4921 accatttaaa accaaatatt tgcttggtgt tgtgcgtgca tggcgggcga aaattgtcag
     4981 gaaggtgaca ggggccacat gggctcacgt gggctttcgg gtaaaaatcg cttgcaatta
     5041 catcgcacgc aatataaatt gccgacatgg cagacacacc acccctctcc accaccggca
     5101 acccggcgct gaaactggac gcccagctgt gctttgcact gtattcgacc ctgttggggg
     5161 tcaataaggt gtatcgcacc ctgctgcgcg agctggacct cacctacccg caatacctgg
     5221 tgatgctggt gctgtgggag cgcgacgagc gcaatgtgtc ggacatttgc agccagctct
     5281 acctggaaac cgccacgctg acgccgctgc tgaaacggct ggaagcgcgc gggctggtca
     5341 accgccgccg ctcacccagc gacgagcgtc aggtgattgt cagcctgagc gaggccgggc
     5401 gcgcgctgta cgcccaggtg cagcatattc ctggctgcat gagcgaggcg ctgtcttgtt
     5461 cgcaggacga cattgtcgcc ctgcgcaagc aactgctgac cttgcgcgac gccttgtttc
     5521 agcacgtctg ag
//
LOCUS       DAOPYX010000206         5516 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_18532_length_5516_cov_9.103278, whole genome shotgun sequence.
ACCESSION   DAOPYX010000206 DAOPYX010000000
VERSION     DAOPYX010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 5516)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5516)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5516
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18532_length_5516_cov_9.103278"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            4..1698
                     /gene="rpsA"
                     /locus_tag="PKN55_10400"
     CDS             4..1698
                     /gene="rpsA"
                     /locus_tag="PKN55_10400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011136593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HNX71699.1"
                     /translation="MTTSTQESFAALFEESLALQEMRAGEVITAEVVNVDQNFVTVNA
                     GLKSESLISVDEFKNDKGEVDVKIGDFVTVAIDSLENGYGETKLSREKAKRMAAWIEL
                     EEALETGKILSGVISGKVKGGLTVMVNSIRAFLPGSLVDTRPVKDTTPFEGKQVEFKV
                     IKLDRKRNNVVVSRRAVLEETLGEERKALLESLTEGAVVKGIVKNITDYGAFVDLGGI
                     DGLLHITDLAWRRVKHPSEVLAVGDEVEAKVLKFDQEKNRVSLGLKQLGEDPWVGLSR
                     RYPQGTRLFGRVTNLTDYGAFVEIEQGIEGLVHVSEMDWTNKNVHPSKVVALGDEVEV
                     MILEIDEDRRRISLGMKQCQSNPWDDFAANYKKGDKIRGTIKSITDFGVFIGLPGGID
                     GLVHLSDLSWHVAGEEAVRNFKKGDEVEAVVLSIDVERERISMGIKQLEGDPFSNFVA
                     MNDKNSLVKGTVKSMDAKGAVIQLEADVEGYLRASEVSRDRVEDIRTHLKEGDEVEAL
                     IINVDRKSRSIQLSIKAKDAAEEAGAMSKLQSTDSATAGTTNLGALLKAKLSGSNE"
     gene            1711..2001
                     /locus_tag="PKN55_10405"
     CDS             1711..2001
                     /locus_tag="PKN55_10405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HNX71700.1"
                     /translation="MTKSELIARLAERYPQLVAKDTELAVKTILDAMATSLSRGQRIE
                     IRGFGSFDLNYRPPRVGRNPKSGSSVEVPEKYVPHFKAGKELRERVDAQVES"
     gene            complement(2247..2468)
                     /locus_tag="PKN55_10410"
     CDS             complement(2247..2468)
                     /locus_tag="PKN55_10410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX71701.1"
                     /translation="MADEHTDLARVLGRIEGKLDMIVTSQHVQSERLDAMDSRLRQVE
                     TQAAKAGALTGGLVAVLTAIVVEWVKRLS"
     gene            complement(2559..2762)
                     /locus_tag="PKN55_10415"
     CDS             complement(2559..2762)
                     /locus_tag="PKN55_10415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX71702.1"
                     /translation="MKLIPNWRQARRYWSVRLAVLTAAVAALESALPALREALPAHWY
                     GLAAVLIALARVVQQTPRHHQQD"
     gene            complement(2775..3215)
                     /locus_tag="PKN55_10420"
     CDS             complement(2775..3215)
                     /locus_tag="PKN55_10420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012696640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mor transcription activator family protein"
                     /protein_id="HNX71703.1"
                     /translation="MTPQHLTQVQHLLPASAQTLADLIGLPATLKLVSELGGRTWSFV
                     KHQRSPRDEGDALARIVGEREAELLTHHFAGDADVHIPLCKAALRELRDREIRARFDE
                     MTMEHHYSARQAVAELAGCYGIASRHVWRLLKQADKEPGTRPLN"
     gene            3523..4275
                     /locus_tag="PKN55_10425"
     CDS             3523..4275
                     /locus_tag="PKN55_10425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012696646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNX71704.1"
                     /translation="MQSPDETHHTDPAHEPERTGFHARLNLLLPGFKSVRAFATASGV
                     SQTGLQRLINGGEPTLSTLVALARAARVSIEWLATGEGPMRRDAEPITHAQPTPPACL
                     DTLGNPVNLDEFVFIPRFNLQAAAGFGSSSDGERPVFSMAFRRYWVQNYLHADARDLS
                     VISVKGDSMEGVLNERDVILINHADTQPRHGLYVLRLNNDLVVKRVQPLPGGRVQVSS
                     ANESYLPFELDLQALGEDVAIIGRVVWFGRQI"
     gene            complement(4285..>5516)
                     /locus_tag="PKN55_10430"
     CDS             complement(4285..>5516)
                     /locus_tag="PKN55_10430"
                     /inference="COORDINATES: protein motif:HMM:NF037955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNX71705.1"
                     /translation="AECSELRLSCFLRECLMPHPRPAARQLWPFAGFYFCYFSFVGVT
                     SPYWGLYLASLGLTPWAIAVLASMPSLARVTAPGFWGWLADRHGQRRRLVRLTTSLAA
                     LSFAGLFLDGRFAWLFAVLALAHFFWAAPLPLVDASVLSRTADSPGAYSRVRLWGSIG
                     FVVMSLLVGYLLEFMPMAQLPWVVLATLLGLAAFAWRLPDTPSPPARHSQQPFMATLR
                     QPVMRRVYLVTVLMAVAHGPYYAFYSIGLKAHGWQASQIGWLWTLGVLAEITAFWWMP
                     RILARVSLAQLLRFGLLMAVLRFAVIAALPDVPAVMMAMQLLHAFTFATHHTASIGLI
                     HQAAPPEHQAKSQGLYWVLGYGLGGGIGGLAAGALWPHGQMLAAFGLSCAAALLGWLC
                     ALRWPTSVQQARTMPSA"
BASE COUNT         1203 a         1710 c         1651 g          952 t
ORIGIN      
        1 ttaatgacta cctccaccca agaaagcttt gccgcgctgt tcgaggaaag cctcgccctc
       61 caggaaatgc gcgctggtga agtgatcacc gccgaagttg tgaacgttga ccagaacttc
      121 gtgaccgtca acgccggcct gaagtccgaa tcgctgatct cggttgacga gttcaagaac
      181 gacaagggcg aagttgacgt caagatcggc gacttcgtga ccgtcgccat cgactcgctg
      241 gaaaacggct acggcgaaac caagctgtcc cgcgagaagg ccaagcgcat ggccgcctgg
      301 atcgagctgg aagaagccct ggaaactggc aagatcctgt ccggcgtgat cagcggcaag
      361 gtcaagggtg gcctgaccgt catggtcaac agcatccgcg ccttcctgcc gggttcgctg
      421 gtggacaccc gtccggtcaa ggacaccacc ccgttcgaag gcaagcaagt cgaattcaag
      481 gtcatcaagc tggatcgcaa gcgcaacaac gtggtggtga gccgccgcgc cgtgctggaa
      541 gagaccctgg gtgaagagcg caaggcactg ctggaaagcc tgaccgaagg cgcagtggtg
      601 aagggcatcg tcaagaacat caccgactac ggcgcattcg tggacctggg cggcatcgac
      661 ggcctgctgc acatcaccga cctggcatgg cgtcgtgtga agcacccgtc cgaagtgctg
      721 gcagtgggcg acgaagtcga agccaaggtg ctgaagttcg atcaagagaa gaaccgcgtg
      781 tccctgggcc tgaagcagct gggcgaagat ccgtgggtgg gcctgtcccg tcgttacccg
      841 caaggcaccc gcctgttcgg ccgcgtgacc aacctgaccg actacggcgc attcgtcgaa
      901 atcgaacagg gcatcgaagg cctggtgcac gtgtccgaaa tggattggac caacaagaac
      961 gtccacccgt ccaaggttgt ggccctgggc gacgaagtgg aagtcatgat cctggaaatc
     1021 gacgaagacc gtcgtcgtat ctccctgggc atgaagcagt gccagtccaa cccgtgggac
     1081 gatttcgccg ccaactacaa gaagggcgac aagatccgcg gcaccatcaa gtcgatcacc
     1141 gactttggcg tgttcatcgg cctgccgggc ggcatcgacg gtctggtgca cctgtccgac
     1201 ctgtcgtggc acgtggctgg cgaagaagct gtgcgcaact tcaagaaggg cgacgaagtg
     1261 gaagccgtgg tgctgtccat cgacgtcgag cgcgagcgca tctccatggg catcaagcaa
     1321 ctggaaggcg atccgttcag caacttcgtg gccatgaacg acaagaacag cctggtcaag
     1381 ggtactgtga agtcgatgga cgccaagggc gccgtgattc agctggaagc cgatgtcgaa
     1441 ggctacctgc gcgcctcgga agtgtcgcgt gaccgcgtgg aagacatccg cacccacctg
     1501 aaggaaggtg acgaagtcga agcgctgatc atcaacgttg accgcaagag ccgctcgatc
     1561 cagctgtcga tcaaggccaa ggatgccgct gaagaagccg gcgccatgag caagctgcaa
     1621 tccaccgaca gcgcgaccgc aggcaccact aacctgggcg ctctgctgaa ggctaagctg
     1681 tccggcagca acgagtaagc agggtaggcg atgaccaagt ccgagctcat cgccaggctc
     1741 gcggaacgct acccgcagct cgtcgcgaaa gacaccgaac tcgccgtcaa gaccattctt
     1801 gacgcgatgg cgaccagtct ttcgcgcggg cagcggattg aaattcgcgg tttcggcagt
     1861 ttcgatctga actaccgtcc gccgcgcgtc ggccgcaacc ccaagtcggg cagcagtgtg
     1921 gaagtgccag aaaagtacgt gccacacttc aaggccggca aggagttgcg cgagcgcgtg
     1981 gacgcgcagg tagaatcgta agcctgtcgg cacgcggtgt tccgtgtgct ggccgcgtaa
     2041 ccgcaactgg ctgtaccaaa acaccacgcc cggcgtctgc cgggcgtggt gttttttttg
     2101 tctgcgccat ccattgaagc atgtccggcc ccacaacctg cacaatgacc acagtggggc
     2161 gtgtctgtca ttctgccgcc ggacatcctg tcatgcctgg catcacgcct gtatgggtga
     2221 tgcccgagtc tctgtctgcg ttgccatcaa ctcaagcgct tgacccattc gaccacaatg
     2281 gctgtcagca cggccaccag gccaccagtc agtgcgccag ccttggcagc ctgggtttct
     2341 acctgccgca gacggctgtc catggcgtcc agtcgttcgc tttgtacatg ctggctggtg
     2401 acaatcatgt ccagcttgcc ttcgatccgg cccagcaccc tggccaggtc ggtgtgttca
     2461 tccgccattc ggcgcctcct tttcattgcc cgcacatgcc gtgttcgcgt gcccggcggc
     2521 gccggccgga aacgcgcaag gtattgtgcc ggcatggttc aatcctgttg atggtgacgt
     2581 ggggtttgct gcaccacgcg tgccagcgca atcaacacgg cggccaagcc ataccagtgt
     2641 gcaggcaaag cctcgcgcag cgccggcagg gcggattcca gcgcggccac tgccgctgtc
     2701 agcacggcca gacgcacact ccagtaacga cgcgcctgcc gccagttcgg aatcagcttc
     2761 atggcatgcc ccgctcagtt cagcggccga gtgccaggct ctttatccgc ctgcttgagc
     2821 agccgccaca catggcgact ggcaatgcca tagcaaccgg ccagctcagc gacggcctga
     2881 cgggcgctgt agtggtgttc catggtcatt tcgtcaaagc gggcgcgaat ctcgcggtcg
     2941 cgcagctcgc gcaaggctgc cttgcacaag ggaatgtgca catcggcatc gccggcaaaa
     3001 tggtgagtca gcagttcagc ctcacgctcg ccgactatcc gcgccagcgc atcgccttcg
     3061 tcacgcgggc tgcgttgatg cttgacgaac gaccaggtgc ggccacccag ctcactgacc
     3121 agcttgagcg tggccggcag accaatcaga tcagccaggg tttgtgcgct ggccggaagc
     3181 agatgctgaa cctgggtaag gtgttgcggg gtcatgttgg atctcctttt caggtattac
     3241 gccgtgacgg aattgccgac agtgcagcaa aacgcgtaat ccggcttaaa ataacgtgtc
     3301 aaacgggagc gatgtgcgcc attggcatgc gtccctgccg tgatatccgg atcgctgtgg
     3361 tgaaccgttg atatcaacga gtaggttcat gttaacggca aatatgagtg atgtaaacca
     3421 cttttgaacg tgtttgctat tatattgaac atgtacgctg acatttatgc tatcattagc
     3481 gtaaactata cgacatagat tcagactatg gatgcaataa acatgcaatc tcctgatgaa
     3541 acgcatcaca ccgaccctgc acatgaacct gaacgtacag ggtttcatgc ccggctgaat
     3601 ctgttgctgc caggcttcaa gagcgtacgc gcatttgcca ccgcctctgg ggtttcgcaa
     3661 accggcctgc aacggttgat caatggcggc gagcccaccc tgtccaccct ggttgcgctg
     3721 gctcgtgcag ccagggtcag cattgaatgg ctggccaccg gtgaaggccc gatgcgccgc
     3781 gatgccgagc caatcacgca tgcccagccc acgccaccgg cttgcctgga cacgctgggt
     3841 aacccggtga atctggatga gtttgttttc atccctcgct tcaaccttca ggctgccgcc
     3901 ggctttggca gcagcagcga tggcgaacgc ccggtgttct cgatggcttt tcgccgctac
     3961 tgggtgcaaa actacctgca cgccgacgca cgggatctct ccgtgatatc ggtcaaaggc
     4021 gactcaatgg aaggggtatt gaacgaacgc gatgtgatct tgatcaacca tgccgacacg
     4081 caacctcgac atggcctgta tgtgctgcgg ctgaacaacg atctggtggt caagcgcgtg
     4141 cagccgctgc ccggcgggcg tgtgcaggta agcagcgcca acgaatccta tctgccattt
     4201 gagctggatt tacaggcgct gggtgaagat gtggccatta ttggccgagt ggtgtggttt
     4261 ggccggcaga tttaagcgcc caggctaagc gctgggcata gtccgtgcct gctggaccga
     4321 cgttggccaa cgcagcgcac acagccagcc cagcaaggcg gcggcgcaag acaaaccaaa
     4381 cgccgccagc atctgcccgt gcggccacag cgcccctgcc gccagcccgc cgatgccgcc
     4441 gcccagacca tagcccagca cccagtacag cccctggctt ttggcctggt gctcgggcgg
     4501 cgccgcctgg tgaatcaggc caatgctggc ggtgtgatgg gtggcaaagg taaaggcatg
     4561 cagcagttgc atggccatca tcaccgccgg cacgtcgggc aaggcggcaa tcacggcaaa
     4621 gcgcaacacg gccatcaaca gaccaaagcg cagcaattgc gccaggctga cccgcgccag
     4681 aatgcgcggc atccaccaga aggcggtgat ttccgccagc acccccagcg tccacaacca
     4741 gccgatctgg ctggcctgcc agccatgcgc tttcaggcca atcgagtaaa acgcgtaata
     4801 cggcccgtgc gccacggcca ttaacaccgt caccagatac acccgtcgca tcaccggctg
     4861 gcgcaaagtg gccataaacg gctgctggct gtgtctggcg ggcgggctgg gggtgtctgg
     4921 caaacgccag gcaaatgccg ccagccccag cagcgtggcc agcaccaccc acggtaactg
     4981 cgccatcggc atgaattcca gcagatagcc caccagcaag ctcatcacca caaagccaat
     5041 cgagccccac agccgcaccc gcgaatacgc gcctggcgaa tcagccgtgc ggctgagcac
     5101 gctggcgtcc accagcggca agggcgccgc ccagaaaaaa tgcgccagtg ccagcacggc
     5161 aaacagccag gcaaaacggc catccagaaa caagccggca aaactgagcg ccgccagcga
     5221 ggtggtcagg cgcaccagcc ggcgccgctg gccgtggcga tccgccagcc agccccaaaa
     5281 acccggcgcg gtcacgcgcg ccagtgatgg catggatgcc agcacggcaa tcgcccaggg
     5341 cgtcagcccc agcgaggcca gatacagccc ccagtaaggc gaggtcacgc cgacaaaaga
     5401 aaaataacaa aagtaaaacc cggcaaacgg ccataactgc cgcgcggctg gccgtggatg
     5461 gggcatcaga cactcgcgta aaaaacaaga taagcgcagc tcactacatt cagcgc
//
LOCUS       DAOPYX010000207         5496 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_18611_length_5496_cov_9.609998, whole genome shotgun sequence.
ACCESSION   DAOPYX010000207 DAOPYX010000000
VERSION     DAOPYX010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 5496)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5496)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5496
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18611_length_5496_cov_9.609998"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            88..282
                     /locus_tag="PKN55_10435"
     CDS             88..282
                     /locus_tag="PKN55_10435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013367171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HNX71706.1"
                     /translation="MFAQLEKLGKYLGQAANLMVGVPDYEVYLQHMRATHPEETPMTY
                     EAFFRERQEARYGGGAGKCC"
     gene            482..1504
                     /locus_tag="PKN55_10440"
     CDS             482..1504
                     /locus_tag="PKN55_10440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014086719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HNX71707.1"
                     /translation="MNPIPVTLLTGFLGAGKTTLLNHILRGAHGQKIAVIENEFGPVN
                     IDSALLIHEASEVIEMTNGCLCCTIRGDLDGHLRDLHARRAAGELAFDRLVIETTGLA
                     DPTPITQTFFASDALIQAYRLDGVIAVVDALHAPAQLAEHAVVRRQIGFADRVLVSKG
                     DLVDEQALAALSARLARINLAAGQYRLAHGEVDLSLLFDLRGFHLDDSVLAAPAAHPA
                     QLYQPALGASRPAFRAPAPAQSFADDIAALHLHHPGALDMLRISAFMNQLMEQHGDAL
                     LRHKGVLAISGEPRRLIFQGVHKIAGFDYGREWGDEAPRSDIVLIGRSLPEAALRAGF
                     AECVAG"
     gene            complement(1560..2951)
                     /locus_tag="PKN55_10445"
     CDS             complement(1560..2951)
                     /locus_tag="PKN55_10445"
                     /inference="COORDINATES: protein motif:HMM:NF012296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HNX71708.1"
                     /translation="MSDAPRCPWRFGAAHAVSTDWPPGPRAGWGGWGLLAELSRDPLA
                     VLARWRAEYGDVVHLRVWPLHQVVISDPALLHEVLVKQHEALTRWPRGMQVFGQLYGH
                     SVFIAEGQAWRDKRRALAAGFSRQAAQDAAPAMAEAAGQVLAGWPSAHPAWRADQALA
                     TLTLEVMARLLFSLELGEAAPTAAQAVRTVLAASHAELFWPASWPDWLPHKRGKRQAR
                     QWLDGLLQRQLHARLALAEAAWPDDLLSRWLRLHRADPNAWPLRAVRDECMTALLAGH
                     ETTGATLAWWMWALARQPEIQAEVSAEACRILRGRAPTVADAPALTALRRSLEETLRL
                     HPAAPLLSTRRAIRPITVGPWRLPAGTLLLLPVQLAQHDPQAFAQPEDFCPARFAGHE
                     ASTHPHWLPFGLGPRVCLGQHQAMMELTVLAALLAQGFSWRAAAGQPAPQPLLQVSCR
                     PDALWLDIRRRVG"
     gene            3197..3919
                     /locus_tag="PKN55_10450"
     CDS             3197..3919
                     /locus_tag="PKN55_10450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX71709.1"
                     /translation="MKFQKTIVPCSRTKDGVSRTKMKSQDILLLLKVVSLSQQANQSA
                     DQFPQNWKDWDLTAVLDEGSAHEAVDEQDGAFSNVAADQPPYDPYSVRGLALSTGISK
                     SEVSMALKRCYANGLAKRDRHSGAPRVNTKSLCEFIVHGLRYVFPAKPGEVTRGIATG
                     FAAPVLQDKLMSAGELVPVWADARGDTKGLAVEPLFKTAPMAVKQDPTLYAFLALVDA
                     IRLGQPRERKLAAQTLETLLKE"
     gene            3921..4520
                     /locus_tag="PKN55_10455"
     CDS             3921..4520
                     /locus_tag="PKN55_10455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX71710.1"
                     /translation="MNGHNKHTDMILAVAQAIGEDMRRQVAFVGGCTTGLLLTDDYTR
                     EQVRHTEDVDLIVHVLGQMGWADLQATLRARGFKDIMADDQSPICAMALGELRVDFMP
                     DDPAILGFSNRWYRQALDTAQDFPLTGEVCIRLVTPAYFVATKLEAYRGRGNNDPLSS
                     QDIEDILNLFAGRHDLTEEIDAAPSRGKKLYRRATWRAA"
     gene            4589..5422
                     /locus_tag="PKN55_10460"
     CDS             4589..5422
                     /locus_tag="PKN55_10460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX71711.1"
                     /translation="MSDWSWPSNKAWPMLADHLSPLTWQWRSQKGAERQENRDACGLF
                     ANAVTTFAVVMDASAQGENGQDFNMFWMTQVIQALAQHPSGPTVEQVLAHMRTAQRML
                     RPPRFLLETACFCALLIRHDLGRAWILACGDCRIGLEKNGQDIAWLSPVHSQANMLGE
                     AFTLEHALSDARHTVTRCLKVKRFTAPEVLEAPFDPASTWLLATDGYWVDQLTGQLGD
                     PHFADDRSLLKLGRSVTPLHASDSDNLRVVGALHTPASCMHSLGISSQNWLSHANQPK
                     H"
BASE COUNT         1048 a         1703 c         1749 g          996 t
ORIGIN      
        1 tgcgcgcctg ctggaaagcc tggcagagcc agcagcccac cgcgcgggaa acgccgttca
       61 ccccgctgcc ggccaaggct taaggccatg tttgcccagc tggagaagct tggcaaatac
      121 ctcggtcagg ccgccaatct gatggtgggc gtgcctgact atgaagtgta tttgcaacat
      181 atgcgcgcca cccacccgga agaaacgccg atgacgtatg aggcgttttt tcgcgagcgg
      241 caagaggcgc gctatggtgg gggcgcaggg aagtgttgtt gacttagcgt ggctcacaaa
      301 accctcctgg cgtcgttacg cgaccttgcc gtacgacctg actgtctgcg gtcgcatgcc
      361 tagccaagat cgcttcgctg ggttttgtga acggctcttg ttgtccgcca tggtcatcga
      421 cgatggcggc tttcccccgc atcatggcgg ggttttcttt ttggcgtgtt gcggagcttg
      481 catgaatccg attccagtca ctttgcttac cggttttctt ggcgcgggca aaaccacctt
      541 gctcaatcac attctgcgcg gcgcgcatgg gcaaaaaatt gccgtgatcg aaaacgagtt
      601 tggcccggtg aatatcgaca gcgccttgct gatccacgaa gccagcgaag tgatcgagat
      661 gaccaatggc tgtctgtgct gcaccattcg cggtgacctg gacgggcatt tgcgcgattt
      721 gcacgcccgg cgggcggcgg gggagctggc gtttgaccgc ctggtgatcg aaaccaccgg
      781 gctggccgac cccacgccaa tcacccagac attttttgcc agcgacgcgc tgattcaggc
      841 ctatcggctg gatggggtga ttgccgtggt ggatgcgctg cacgcgccgg cgcagttggc
      901 cgagcatgcc gtggtgcgcc ggcagattgg ctttgccgac cgggtgctgg tgtccaaggg
      961 cgatttggtg gatgagcaag cgctggcggc cttgagcgcg cggctggcgc gcatcaacct
     1021 tgccgccggg caataccggc tggcgcatgg cgaggtggat ttgagcctgc tgtttgatct
     1081 gcgcggtttt catctggacg acagcgtgct ggcggcgccg gcggcgcatc cggcgcagtt
     1141 gtatcagccg gcgctgggcg ccagccggcc agcgtttcgc gcgccagcgc cggcgcagtc
     1201 gtttgccgac gacatcgccg cgctgcattt gcatcacccc ggcgcgctgg acatgctgcg
     1261 catcagcgcc ttcatgaacc agctgatgga gcagcacggc gacgcgctgt tgcgccacaa
     1321 gggcgtgctg gcgatttccg gcgagccgcg ccggctgatt tttcaggggg tgcacaagat
     1381 tgccggcttt gactacggcc gcgagtgggg cgatgaagcg ccgcgctcgg acatcgtgct
     1441 gattggccgc agcctgccgg aagcggcgct gcgcgccggc tttgccgagt gcgtggcggg
     1501 ctgaaaaccg cttggccagg ctgtcgggca acaaagccag gaggctttgt aagcgactct
     1561 tagcccacgc gccggcggat atccagccac agcgcatccg gccggcacga cacctgcaac
     1621 agcggctgcg gcgctggctg gccggcggcg gcgcgccagc tgaagccttg cgccagcagg
     1681 gcggccagca cggtgagctc catcatcgcc tggtgctggc ccaggcacac gcgcggaccc
     1741 aggccaaacg gcagccagtg cggatgcgtg ctggcttcgt gcccggcaaa gcgcgccggg
     1801 caaaaatcct ccggctgggc aaacgcctgc gggtcgtgct gcgccagctg caccggcagc
     1861 agcaacagcg tgccagccgg cagccgccac gggcccacgg tgatcgggcg gatggcacgg
     1921 cgggtgctca gcagcggcgc cgccgggtgc aggcgcaggg tttcttccag cgagcggcgc
     1981 agcgcggtca gcgcgggcgc atccgccacc gtcggcgccc gcccacgcag gatgcggcag
     2041 gcttcggcgc tgacctcggc ctgaatctcg ggctggcgcg ccagcgccca catccaccag
     2101 gccaatgtcg cgcccgtggt ttcgtgcccg gccagcaagg cggtcatgca ttcgtcgcgc
     2161 acggcgcgca gcggccaggc gttggggtcg gcgcggtgca ggcgcagcca gcggctgagc
     2221 agatcgtccg gccaggcagc ctcggccagc gccagccggg cgtgcagctg gcgctgaagc
     2281 aagccatcca gccactgccg ggcctggcgc ttgccgcgct tgtgcggcag ccagtccggc
     2341 cagctggccg gccagaacaa ctcggcatgg ctggcggcca gcacggtgcg caccgcctgc
     2401 gcggcggtgg gcgctgcctc gcccaactcc agtgagaaca ataaacgggc catcacctcc
     2461 agcgtcagcg tggccaaggc ctggtcggcg cgccaggcag gatgggcgct gggccagccg
     2521 gccagcactt gcccagcggc ctcggccatc gccggcgcgg catcctgcgc cgcctgccgg
     2581 gaaaaccccg ccgccagcgc ccgccgcttg tcgcgccagg cctggccttc ggcaataaac
     2641 acgctatggc catacaactg gccaaacacc tgcatgccgc gcggccagcg cgtcagcgcc
     2701 tcgtgctgtt tgaccagcac ctcgtgcagc aaggccggat cgctgatcac cacctgatgc
     2761 agcggccaca cccgcaaatg caccacatcg ccatactcgg cgcgccaacg cgccagcacc
     2821 gccaaaggat cgcgcgataa ttccgccagc aaaccccaac cgccccaccc ggcgcgcggg
     2881 ccgggcggcc agtcggtgga cacagcatgg gcagcgccaa agcgccaggg acaacgggga
     2941 gcgtccgaca tgatgacctc gcaacaggca tgaacaggcc atcagtatcg ccagccccgg
     3001 cggggtggtc ttgaacgcaa acgacatggc ctatttgccc atgcgcaggc actggggcca
     3061 tcctgcccga cgctttcacc caagtgcgcc ctccgtgtca ggactgcccc aggggcgata
     3121 gcctggctga atcactgttg caaagcatgt atgtgtaagc gaatcagctg gcggcaagta
     3181 ttatttattg acaataatga aatttcaaaa aacaatcgtc ccttgttcga ggacaaagga
     3241 cggcgtgtcg cgcacaaaaa tgaaaagtca agacatacta ctgctgttaa aagtggtgag
     3301 cctgtcccag caagcaaacc agagcgccga ccagtttcca caaaactgga aagactggga
     3361 tctcacggcc gtgctggatg aggggagcgc ccacgaggcc gtagacgaac aagacggggc
     3421 gttctcgaat gtggcggcag accagccgcc atatgaccct tatagcgtgc gagggctcgc
     3481 tttatcgacg ggcatcagca aatccgaggt gagcatggcg ctgaagcgct gctatgctaa
     3541 tggtctggca aaacgagatc gccacagcgg tgcgccacgc gtcaacacaa agtctttgtg
     3601 cgaatttatc gtccatggtt tgcgttacgt ctttccggcc aagcccggag aggtgacgcg
     3661 gggcattgcc actggctttg ccgcgcccgt gttgcaagac aagctgatga gcgccggcga
     3721 gcttgtgccg gtgtgggccg atgcgcgtgg agacacaaaa gggcttgcgg tcgagccctt
     3781 gttcaagaca gcgcccatgg cggtcaaaca agaccccact ctctatgcat ttctggcgct
     3841 ggttgacgcc attaggctgg gacagccccg cgagcgtaag cttgccgccc agacactaga
     3901 aaccttgctc aaggagtgat atgaacggcc acaacaagca cacagacatg attcttgcgg
     3961 ttgcccaggc cattggcgaa gacatgcgtc gtcaggttgc ctttgtgggc ggatgcacaa
     4021 ccggcctgtt gctgacagac gactacaccc gcgagcaagt ccggcatacc gaggatgtgg
     4081 atttgattgt gcatgtgctt ggccagatgg gctgggctga tttacaagcc acactgcgcg
     4141 cccggggctt caaagacatc atggctgacg atcagagccc catctgcgcc atggccttgg
     4201 gtgagttgcg ggtggatttc atgcccgacg accccgccat tcttggcttt tctaatcgct
     4261 ggtatcgcca ggcgctggac accgcgcaag actttcccct caccggggag gtctgtattc
     4321 gcttggtcac gcccgcatac tttgtggcga caaaacttga agcctatcgt ggacggggca
     4381 acaacgaccc actgtcgagc caggacattg aagacattct gaatctgttt gccgggcgac
     4441 atgatttgac cgaagaaatt gacgctgccc cctcaagagg taaaaagcta tatcgccgag
     4501 caacttggcg cgctgcttga cgatggcgat ttcgactatg ccgtacaagc ggcggcgctg
     4561 ggtgatgcgt cacgcgaaga ctggatctat gagcgactgg agctggccat caaacaaggc
     4621 atggccgatg ctggctgacc atctgtcgcc tttaacatgg caatggcgca gccaaaaagg
     4681 agcagaacgt caagaaaacc gggatgcctg tggcttgttt gccaacgcgg tcaccacgtt
     4741 tgctgtggtg atggatgcct ctgcacaagg cgaaaacggg caggacttca atatgttttg
     4801 gatgacacag gtaatccagg cgctggcgca acacccctcc gggccgacgg tagaacaggt
     4861 gctggcgcat atgcgcaccg cgcagcgcat gctgcgcccg cctcgttttt tgctggaaac
     4921 agcatgcttt tgcgccttgc tgattcggca tgatcttggc cgtgcatgga tattggcgtg
     4981 cggggattgc cggattggac tggaaaaaaa cggccaggac atcgcctggc tgtctccggt
     5041 tcactcacag gccaatatgc ttggcgaagc ttttaccctg gaacatgcgc tctctgatgc
     5101 acgacatacc gtaacgcgtt gcctgaaggt aaagcgtttt acggcgcctg aggtcttgga
     5161 agcgccattc gatccagcgt ctacctggct gttggccacc gatggctatt gggttgatca
     5221 actgacagga cagcttggcg acccccattt tgccgatgat cgaagcttgc tcaagttagg
     5281 tcgctcggtg acgccgctgc atgcctcgga cagtgataac ttgcgtgttg tcggcgcctt
     5341 gcacacgccg gcttcttgca tgcatagcct gggcatctcg tcgcaaaact ggctgagcca
     5401 cgcaaaccag ccaaagcact gagaggccaa gaaggacgaa cccgccatgt gcgccgattt
     5461 caccgcctgc cccgacccgc tcgcccgcgc cccgcg
//
LOCUS       DAOPYX010000208         5441 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_18885_length_5441_cov_11.212588, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000208 DAOPYX010000000
VERSION     DAOPYX010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 5441)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5441)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5441
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18885_length_5441_cov_11.212588"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(292..969)
                     /locus_tag="PKN55_10465"
     CDS             complement(292..969)
                     /locus_tag="PKN55_10465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX71712.1"
                     /translation="MKKAKIAATLLALSMAAGHQAHAFGLGDLTKAVTGATASTSNVS
                     GDDVDAFIQTARQADQLINQSARLIYLALASQEEKAKHEAAIKAANEMTDVSEKKAKL
                     DEINADTATALQKTLNNQETINSINKMSAAQRKNLAGAAFNFMLGLMKDKELAQRGSG
                     LVSGVSNNPMLAPKLVNLKEAVDSVSGQVKNGVKIADGLVSMAKAGNMKIMPTSTSDK
                     PVAVSEL"
     gene            complement(986..>1453)
                     /locus_tag="PKN55_10470"
     CDS             complement(986..>1453)
                     /locus_tag="PKN55_10470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX71713.1"
                     /translation="LAYCPNPKSQVGSQFVPRYDELAYAVAAQTGFNMVNRSLKGLVE
                     RRVGSASGFKANIKLPEAAFDQCLESLYRIDPVDSPCEGDACSTRYKVKTAFRQKQGD
                     TVSQQMILEHTFKTSGYQQNIDSTQLGQLQRAELYEDVGELLKQTAKNLFQTK"
     assembly_gap    1454..1553
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1554..2608)
                     /locus_tag="PKN55_10475"
     CDS             complement(<1554..2608)
                     /locus_tag="PKN55_10475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX71714.1"
                     /translation="MLASAGETIHVYPVPGIFFDEGADNQKGVASKISPEIGKVLKAN
                     IRQNVGYAGQAIAKNFNNLTQQIDAKNRYRTLAVSVQVTRASRFEVNKKDGTRDIYLP
                     LTLSLYFSNPLTGEVLQSFNQTRVTTFTSTPDTIAAKISQYTQQSFERTLDELLTHAA
                     SQFKPYVVEATVKETWKGYGILDKGYAAGIGKDDILLDTDGNEIKIEHAGQDYAVATP
                     IGGKISSGGRYARTSMMKLSDVKKPRVLVVVSDGNADIPDAVMSQLFADQLGADAQFA
                     VLPLNANYGKVQSAIDSNTQIGSAVSGQRELPDYFIRLVVPDVVEYEKPTNLTYKTQR
                     HYKSWAFAELLAKNGQVL"
     gene            complement(2656..4239)
                     /locus_tag="PKN55_10480"
     CDS             complement(2656..4239)
                     /locus_tag="PKN55_10480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX71715.1"
                     /translation="MKKTTLALALATILASTFAHALEIETRGVGTAQKSLFSNAADIR
                     GAAMRQAKRNAVLAALDKVVGAGTRNNPKVQEQLEELIAQVGEQVFYDPMPDMVGDTY
                     RINITLRMDDKQLRSVISDLGLALNTNTVRSQSFLVLMDEFFTTPTDLRAPLEELIEY
                     KHEAGNSYKEKEAAARSSKSAYVASDKAAYAVKGASASTVNAGYDSRVAAAQNDGQGG
                     KAAVAARDSGRYSAASGSTYQAAGARSTTVAAASSSSAAYAHQVNAEEHDNTYFKKLV
                     KYQPQNTGPDKKNYTYNELKGQFVDLDIKVIDNTLFRSRYFGNKPVTLDKLENSAELE
                     KYVSFARKEATADYFMLGTSVIYDRGIDNNSGMHACTGVASTKNFSTKTGEDIGSSTQ
                     SENAVGQTSDDCRAKLAIKLSQSLASQVGKRVQDYYKRRTMYGSEYIVRLTGSNISLM
                     TRVAFSQALKNVPALENQVQRQANDRQVEIVAAYKGSDPIDQAIAMALASDQQFSRLD
                     SRTDGNIITLCMNGCGKGK"
     gene            complement(4254..4757)
                     /locus_tag="PKN55_10485"
     CDS             complement(4254..4757)
                     /locus_tag="PKN55_10485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX71716.1"
                     /translation="MNTYQALLISLICLAGCNESRNTANQASPAPAAEASQSKVTLDA
                     VRNELMKHNFGQASQLAEQLTAASPSVVEAWVVAAEAKAANQNRLAALAALEQAFNHG
                     LREVSLIEQSRYLADIRNGEEYRTLLHRFGLRTHTTQAAVGDVSIVQGGGVTEVRAGD
                     VSVKLPD"
     gene            5231..>5441
                     /locus_tag="PKN55_10490"
     CDS             5231..>5441
                     /locus_tag="PKN55_10490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX71717.1"
                     /translation="MSEKTHSSGWVWISTEDSIQRGQISRLALLEIPLAMAFFWWLSS
                     LSPWPWLTLIGLMAAPVLLLRSPESV"
BASE COUNT          984 a         1498 c         1523 g         1336 t
ORIGIN      
        1 ttgcttgcat cgtcataggc catccacgct caatgggctg atgttcgcag atcagcagcc
       61 ttcgagccag ctgttgggga ataaatcagc gtatccttag cggccgcgca cctgaccagg
      121 cgcgcgaccg ctaagtttta tgtggcgccg ctcacacacc tctcctggcg tcgttgcgcg
      181 gccttgccgt acaccttgta ctgtctgcgg ccgcatgcct ggccaggcgc gctacgctga
      241 gttttgttaa ccgctcttag cgacgcttgg ccacactttg tagctcggtg tttacagttc
      301 agacactgct accggcttgt cagaagtgga tgtcggcatg atcttcatat tgccggcctt
      361 ggccatactg accaagccat cggcgatctt gacgccattt ttgacttggc cgctgaccga
      421 atcgacagcc tctttcaaat tgaccagctt gggtgccaac atcgggttgt tgctgacgcc
      481 gctcaccaga cccgagccgc gttgggccag ttctttgtct ttcatcaggc caagcatgaa
      541 gttgaaggcg gcgcccgcca ggtttttacg ctgtgctgca ctcatcttgt tgatgctatt
      601 gatggtttcc tgattgttca gcgtcttttg cagcgcggtg gccgtgtcgg cattgatttc
      661 gtcaagtttg gccttttttt cagacacatc ggtcatctca ttagccgctt ttatggccgc
      721 ttcgtgcttg gccttttctt cttggctggc cagcgccaga tagatcaacc gggccgactg
      781 gttgatcaat tgatcagcct ggcgggcggt ctggatgaat gcatccacat cgtcaccaga
      841 cacattgctg gtgctcgcgg tggcgccggt gacggcttta gtcaggtcac ccaggccaaa
      901 cgcgtgcgct tggtggccgg cggccatgga cagagccaac agggttgctg caatttttgc
      961 ttttttcatg atgtgccttg gtttgctact tggtttgaaa aagatttttt gcggtttgtt
     1021 tgagcaattc gccaacatcc tcatacaact ctgcccgttg caattggcca agctgggtgc
     1081 tgtcgatatt ttgctgataa ccgctggtct tgaaggtatg ttcaagaatc atctgttgac
     1141 tgaccgtgtc accctgcttt tgccggaatg cggttttgac tttgtaacgg gtgctgcacg
     1201 catcaccttc gcatgggcta tcgacaggat caatccggta cagtgattcc agacattgat
     1261 caaatgccgc ttccggcagc ttgatattcg ccttgaagcc actggcgctg ccaacccggc
     1321 gttccaccag gcctttgagt gaacggttga ccatattgaa gcccgtttgg gccgccaccg
     1381 cataggccag ctcatcgtag cggggcacaa actggctgcc gacctggctt ttgggattgg
     1441 ggcagtaggc cagnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnagcacct
     1561 ggccattttt tgccagcagc tcggcaaatg cccacgactt gtagtgacgc tgtgttttgt
     1621 aagtaaggtt ggtcggtttt tcgtactcga ccacatcggg gacaaccagc cggataaagt
     1681 aatcaggcag ctcacgctgc ccgctgacag ccgagccgat ttgggtgttg gagtcgattg
     1741 cggactgaac cttgccatag tttgcattca gcggcagcac ggcaaactgg gcatccgcgc
     1801 ccaactgatc ggcaaacagc tggctcatca cggcatccgg aatatccgca ttgccatcgc
     1861 tgaccaccac cagcacacgc ggcttcttga cgtcggacag cttcatcatc gacgttctgg
     1921 cgtagcggcc gccagaggaa atcttgccgc caatcggcgt ggcgacggcg taatcctggc
     1981 ccgcgtgctc aatcttgatc tcgttgccat cggtatccag caggatatcg tccttgccaa
     2041 tcccggccgc atagcctttg tccagaatgc cgtagccttt ccaggtttcc ttgaccgtcg
     2101 cctcaaccac atagggcttg aactggctgg cggcatgggt cagcagttca tccagggtgc
     2161 gctcaaagct ctgctgggtg tactggctga tcttggcggc aatggtgtcg ggcgtgctgg
     2221 taaaggtggt gacccgggtc tggttgaacg attgcagcac ctcaccggtc agcgggttgc
     2281 tgaaatacag gctgagcgtt aacggcaggt agatatcgcg ggtgccgtct tttttattga
     2341 cttcaaagcg tgacgcgcgg gtcacctgca ccgaaaccgc cagcgtgcga tagcggtttt
     2401 tggcatcaat ctgctgggtc aggttgttaa agtttttggc aattgcctgg cctgcatagc
     2461 cgacgttctg ccggatattg gccttgagca ccttgccgat ttcagggctg atcttgctgg
     2521 cgacgccttt ttgattgtcc gcgccttcat caaaaaaaat accgggcacc gggtaaacat
     2581 gaatggtttc cccggcgctg gccaacattg gcgccagcgc aagaaacaaa gcactgacaa
     2641 gtaatttttt attcatcact tgcctttgcc gcagccgttc atgcacagcg taatgatatt
     2701 gccgtcggtt ctggaatcaa gacgggaaaa ttgctgatcc gacgccagcg ccatggcgat
     2761 ggcctggtca atcgggtcgc tgcccttata cgccgcgaca atttcaacct gccggtcgtt
     2821 ggcctgacgt tgcacctgat tttccagtgc gggcacattt ttcagcgcct ggctgaaggc
     2881 tacgcgggtc atcagcgaaa tattgctgcc agtcagacgg acaatatatt cactgccgta
     2941 catcgtgcgg cgcttgtaat agtcttgcac gcgcttgcca acctggctgg ccaggctttg
     3001 cgacagtttg atcgccagtt tggcacggca atcatccgag gtctggccaa ccgcgttttc
     3061 cgactgggtg ctcgagccga tgtcttcgcc ggttttggtc gagaagttct tggtgctggc
     3121 caccccggtg caggcgtgca tgccggagtt gttgtcgatg ccgcgatcgt agatcaccga
     3181 agtgcccagc atgaagtaat cggcagtggc ttcttttctg gcaaagctga cgtatttttc
     3241 cagctcggcg ctgttttcca gcttgtccag cgtcaccggc ttgttgccga aataccggct
     3301 gcggaacagc gtgttgtcga tcaccttgat gtccagatca acaaactggc ctttcagctc
     3361 gttgtacgtg tagttctttt tgtccgggcc ggtattttgc ggctggtatt tgaccagctt
     3421 cttgaagtag gtgttgtcgt gctcttccgc gttgacctga tgcgcgtagg ctgcgctgct
     3481 ggaggaggca gcagccacgg tggtgctgcg cgcgccggca gcctgataag tgctgccact
     3541 ggcggcggaa tagcgcccac tgtcacgtgc ggccaccgcc gctttaccgc cctgcccgtc
     3601 gttttgggca gcggccaccc ggctgtcgta cccggcgttg accgtgctgg cagacgcgcc
     3661 cttgacggca taggcggcct tgtcggaagc cacatacgcc gatttactgg agcgggcagc
     3721 ggcttctttt tctttatacg agttgccggc ttcgtgcttg tattcgatca gctcttccag
     3781 cggtgcgcgc agatcggtcg gcgtggtgaa aaattcatcc atcagcacaa gaaaagactg
     3841 gctgcgcacc gtgttggtgt taagagccag gcccaaatcg ctgatcacac tgcgcagttg
     3901 cttgtcatcc atgcgcaagg tgatattaat gcgatacgta tcgcccacca tgtccggcat
     3961 cgggtcgtag aacacctgct cgccaacttg ggcgatcagc tcttccagtt gttcttgcac
     4021 cttggggttg ttgcgggtgc ccgcgccaac cactttatcc agcgcggcca gcacggcatt
     4081 gcgttttgcc tgccgcatgg cggcgccacg aatatcggcg gcgttgctga acaggctttt
     4141 ctgggcagtg cccacgcccc gtgtttcaat ttccaatgca tgggcaaagg tgctggccaa
     4201 tatggtggca agggccagcg ccagtgtggt ttttttcatg ctaaatccag tatttagtcg
     4261 gggagtttta cggaaacatc gccagcacgc acttcggtga cgccgccacc ttgcacgatg
     4321 gacacgtcac ccactgcggc ttgggtggtg tgggtgcgca ggccgaagcg atgcagcagg
     4381 gtgcgatatt cttcgccgtt gcgaatgtcg gcgaggtagc ggctctgctc gatcaggctg
     4441 acttcgcgca agccgtggtt gaacgcctgt tcaagcgcgg ccagggcggc aaggcggttt
     4501 tggttggcgg ccttggcttc ggccgccacc acccaggctt cgaccacgct gggcgaggcc
     4561 gccgtcagtt gctcggcaag ctggctggcc tggccaaagt tgtgcttcat cagctcattg
     4621 cgcaccgcat cgagggtaac cttgctttgg ctggcttcgg cggcaggcgc cgggctggcc
     4681 tggttggcgg tattgcgaga ttcattgcag cccgccaggc aaatcagcga gatcagcagt
     4741 gcctgatatg tgttcatgtt tcttctactg cttatgagtg gatgcggatg gtgattaagc
     4801 gcggccaaca aaactcagca aagcgatcct ggctaggcac gcgaccgcag acaccgtaag
     4861 gtcgtgtcac aacgccagga gggttttgtg agccgcgcca agaggcacaa gcgtagcgag
     4921 tgcaggcgta agcacgttag cttacttatt ttgcaaaaat cgcattatct tgctctgatt
     4981 tggcatatat tcatatacgt agaaatactt aaatatataa ttaatagtca ttctctacat
     5041 ctgcgcagcg gccctggcgc tgagagcgcc ctcatccgca tcgcccacct ccagcatgac
     5101 ttgtcatccg atgatggcaa gtggcatctt taccaagatg ctcggatttc atcctgcacc
     5161 gcccagcggc gcctgagatt tatccgattc cgtcataaac cgtgcatttt gtcttggcag
     5221 agaaaccttg atgagcgaaa aaacccattc atctggctgg gtgtggataa gcaccgaaga
     5281 ctctatccag cgcgggcaga tttcccggct ggcgctgctg gaaatccccc tggccatggc
     5341 ctttttctgg tggctgagca gcctgtcccc ctggccctgg ctcaccctca tcggcctgat
     5401 ggccgccccc gtgctgctgc tgcgctcgcc ggagtcggtg g
//
LOCUS       DAOPYX010000209         5330 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_19457_length_5330_cov_9.546730, whole genome shotgun sequence.
ACCESSION   DAOPYX010000209 DAOPYX010000000
VERSION     DAOPYX010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 5330)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5330)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5330
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19457_length_5330_cov_9.546730"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            381..1457
                     /locus_tag="PKN55_10495"
     CDS             381..1457
                     /locus_tag="PKN55_10495"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNX71718.1"
                     /translation="MTLWPVLSRIVWPGLLALGLAGCDWLTPSVDAVPVQWYEPVQAL
                     VVAHDTGRVRALREADIQPNESGRVQWHLPVGARVAAGDVLVRLQPDKVDLAREHAEA
                     ALAVARDQSRRTQGALAEARDSLIQAKTPEQIKQARMQLGLAQAAERDQQLALARAEL
                     ALKAAGQALARTLLLAPFAGMVSDIYAGVGEKVSPALPLISLADAASLRVAVEFPLNP
                     QLSVGLPCLLILDAHPDERLLGEIVNMTPLGASPRVSVDVQVPAHAAWPVDSPVKVQC
                     LQRRPHIDEWRSRLVAPKHALRGEGRHRQLWKVTQNHVQRVEVEAGATHGDWVEVQGA
                     LHAGDRVVLAPSAKLTDGASVRLR"
     gene            1462..1995
                     /locus_tag="PKN55_10500"
     CDS             1462..1995
                     /locus_tag="PKN55_10500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX71719.1"
                     /translation="MTTVPGGEPPGHLLTIFTLLWLVPLLTWRLHARWRRLTARQRIS
                     PRRTQIHLSLFTTLLAALAWLWRAQPSVLLHLAGGAVLGLALAWLSWRRTRLEPTRQG
                     IFYQPDRWLGGSVFLFTVSQLGYRMLETLWLSPGLSWAEASARGLTPACLGLWLTHQL
                     GYTLALARYRRQRLAAR"
     gene            2068..3570
                     /locus_tag="PKN55_10505"
     CDS             2068..3570
                     /locus_tag="PKN55_10505"
                     /inference="COORDINATES: protein motif:HMM:NF015406.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase/cryptochrome
                     family protein"
                     /protein_id="HNX71720.1"
                     /translation="MNAADPVHLVWFKRDLRTVDHAALAAACRAGPVLPLYIAEPSLW
                     QGDDASAWQWGFVEESLTALGKALAELGLPLLRLRGEVVDVLEALRQALPLAAIHSHQ
                     ETGNALSYARDRAVAQWSRAHDIAWHEYPQMAVFRGGKRGHWPSRREAFVSAPPATLP
                     ERIIAADIPPLAHAPARWRLFDPGPCRRQRGGRGLGLALLSGFLDERAARYRHGLSSP
                     LTAVDACSRLSPHLAWGTLSLREVTQATRARLARLDAFDPHAQRLAHGLHAFESRLAW
                     HCHFIQKLEQRPELEHHAPLAAVDALRADPNPQWLAAWRDGHTGYPLVDACMAMLRAT
                     GWLNFRMRAMLMSFASYNLWLPWQLTGQHLARLFVDYEPGIHWPQAQMQSGSTGRNAL
                     RIYDPVKQALELDPHGKFVARWLPVLGKVPLAWRFEPWKMPLDVQMHCGVRIGHDYPA
                     PCVPWEPTQRAAREQLRACQPPREARIITPAAPARRSRHQLPPQQLALGF"
     gene            complement(3655..5307)
                     /gene="leuA"
                     /locus_tag="PKN55_10510"
     CDS             complement(3655..5307)
                     /gene="leuA"
                     /locus_tag="PKN55_10510"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019922085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HNX71721.1"
                     /translation="MLRQPQHKYPAFVPLALPDRQWPNQITRTPPVWLSTDLRDGNQA
                     LIEPMSAARKRQLFALLVEIGFKEIEVAFPSASQTDFDFVRQLIEQDAVPADVTLSVL
                     TQSREDLIARTIQALHGARRAIVHLYNPIAPAFRRIVFNSDRAGVKALAVRGAEQIRA
                     LTARCPDTEWVFEYSPETFSACEPEFALEVCDAVSAVWQPTPQHKMIINLPATVELST
                     PNTYADQIEWMHRHLARRDSLILSVHPHNDRGTAVAAAELAMMAGAERVEGCLFGNGE
                     RTGNVDLLTLAMNLYSQGVAPGLDFSDIDRVRAVVEDCTQLPVHPRHPYAGDLVYTAF
                     SGSHQDAIKKGFAQQHADQAWTVPYLPIDPADVRRGYDAVIRVNSQSGKGGVSYLLQQ
                     TYGLALPRRLQIEFSRVVQAYSDTTGGEVDAAALHRLFSAEYLDIDSPYAYASHQQHD
                     NDGRTRLSVTLRRQGEPYALHGEGNGPIDAFVAALGLPLTVLDYHEHAIGAGADARAA
                     CYVELRLGESAGVFGVGVDSHIVAAALKAVLSALNRQLRAGR"
BASE COUNT          878 a         1743 c         1719 g          990 t
ORIGIN      
        1 aagtacggca aggtcacaca acaacgccag ggggttttgt tagcggctct aagccgatgg
       61 agatggcgta tgcctgtgtg cccatgtaca ccggatatgc ccatggcgaa tgttgttctg
      121 ctgatgattt acacaaacag caaacgtaaa aacacgtaaa atccgtatga ctgtactttt
      181 ttcctgttct gcctaaaaaa tacgcggcag cggaaagccc ctctctgaca atcagttagc
      241 gcatttatcc acagccagtg cacaacactg tccgcactga atggggatca agtcagcgta
      301 tcttcccgag gcggccggct gttcgttgct ctgcctgttg cctgtttctg attcgtgtcc
      361 aaatccatga agggtacgcc gtgaccttgt ggcccgtatt gtctcgcatt gtctggcccg
      421 gcctgctggc cctgggtctg gctggctgtg actggctgac cccatcggta gatgccgtgc
      481 ctgtgcaatg gtacgagcct gtacaggcac tggtggtggc gcacgatacc ggacgggtgc
      541 gtgcgctgcg cgaagcagac atccagccca atgaaagtgg gcgggtgcag tggcatctgc
      601 cagttggtgc acgggtggcg gccggcgatg tgctggtgcg cttgcaaccg gataaagtgg
      661 atctggctcg tgaacatgcc gaagcggcac tggcggtggc gcgcgaccaa tcccgacgca
      721 cccagggtgc tctggcagag gcgcgcgaca gcctgattca ggcaaaaaca cctgaacaaa
      781 tcaaacaagc gcgcatgcag ctgggtttgg cccaggctgc cgaacgtgac cagcaactgg
      841 ccctggcacg tgccgaactg gcactcaagg cagctggaca ggcattggcc cgtaccctgt
      901 tgctggcgcc atttgccggc atggtcagcg atatctatgc cggagtcggc gaaaaagtat
      961 cgccagcctt accgttgatc agcctggccg atgcagccag cctgcgcgtg gcagtagagt
     1021 ttccgctgaa tccacagctt tccgtgggtt taccctgcct gttgattctc gacgctcacc
     1081 cagacgagcg tctgctgggc gaaatcgtca atatgacgcc actgggcgcc tcccccaggg
     1141 tcagtgtgga cgtccaggtg ccggcccatg cagcctggcc ggtcgatagt ccggtgaagg
     1201 tgcaatgtct gcaacgccgc ccccacattg atgaatggcg cagccgtctg gtcgccccca
     1261 agcatgcctt gcgcggagaa ggccgtcatc gtcagctgtg gaaggtcacg caaaaccatg
     1321 tgcagcgtgt ggaggtggaa gccggcgcca cacatggcga ttgggtagag gtacaaggag
     1381 cattgcacgc aggcgatcga gtggtgctgg cgccttccgc caagctgacg gatggcgcct
     1441 cggtgcgtct gcgctgacac catgaccacc gtgcccggag gcgaaccccc tggccacttg
     1501 ttgaccattt ttaccctgct gtggcttgtt ccgttgctga cctggcgctt gcacgcgcgc
     1561 tggcggcggc tgactgcacg ccagcgcatc tcgccgcggc gcacgcagat tcacctgagc
     1621 ctgttcacca cactgctggc cgccttggcc tggctatggc gtgcacagcc atccgttttg
     1681 ctgcatctgg ccggcggcgc ggtgctaggt ctggcgctgg cctggctgag ctggcggcgc
     1741 acccgccttg agcccacccg gcagggcatt ttttatcagc ctgaccgttg gctgggcggc
     1801 agtgtgtttt tgttcaccgt cagccagttg ggctaccgca tgctggaaac gctgtggctg
     1861 tcgcctggcc tgtcatgggc cgaggccagt gcgcgcggac tgacgcctgc ctgcctggga
     1921 ttgtggctga cgcaccaact gggctatacg ctggcgctgg cccgctatcg gcgccaacgc
     1981 ctggcggcac gctgactgtt gcattcttgc cgatcggtac tgcgccagac ttgccgtcgc
     2041 gggtttttct gcgttactgt cacgctcatg aatgccgctg accctgttca tcttgtctgg
     2101 ttcaagcgcg atctgcgcac tgtggaccac gccgccctgg ccgccgcctg tcgcgccggg
     2161 ccggtgttgc cgctgtatat cgccgagccc tcgctctggc agggcgacga cgcctcggcc
     2221 tggcaatggg gctttgtcga agaatccctg actgcgctgg gcaaggcgct ggccgagttg
     2281 ggcctgccgc tgttgcgcct gcgcggcgag gtggtggacg tgctggaggc gctgcgccag
     2341 gccttgccgc tggcggccat ccacagccat caggaaaccg gcaatgcgct cagctacgcc
     2401 cgcgaccgcg ccgtggcgca gtggagccgc gcgcacgaca tcgcctggca tgaatacccg
     2461 caaatggcgg tgtttcgcgg cggcaaacgc ggccactggc ccagccggcg cgaggccttt
     2521 gtcagcgccc cgccagccac gctgcccgag cgcatcatcg ccgccgacat tccgccgctg
     2581 gcgcacgcgc cggcgcgctg gcgactgttc gaccccggcc cctgccgtcg ccagcgcggc
     2641 gggcgcggcc tgggcctggc gctgctgtcg gggtttctgg atgagcgcgc cgcgcgctat
     2701 cgccacggcc tgtccagccc gctgactgcg gtggacgcct gttcgcggct gtcgccgcat
     2761 ctggcctggg gtacgctgtc gctgcgcgaa gtcacccagg ccacccgcgc gcgcctggca
     2821 cgactggacg cctttgaccc gcacgcccag cgcctggcgc atggcctgca cgcctttgaa
     2881 tcgcggctgg cctggcattg ccactttatt caaaaactcg aacagcgccc cgagctggaa
     2941 catcacgccc cactggcggc ggtggacgcg ctgcgcgccg accccaaccc gcaatggctg
     3001 gccgcctggc gcgatggcca caccggttat ccgctggtgg acgcctgcat ggccatgctg
     3061 cgcgccaccg gctggctgaa ttttcgcatg cgcgccatgc tgatgagctt tgccagctat
     3121 aatctgtggc tgccctggca gcttaccggc cagcacttgg cgcgcttgtt tgtcgattac
     3181 gaaccgggca tccactggcc gcaggcgcaa atgcagtctg gcagcaccgg ccgcaatgcc
     3241 ctgcgcattt acgacccggt caaacaggcg ctggagctgg acccgcacgg caaatttgtt
     3301 gcccgctggc tgcctgtgct gggcaaagtg ccgctggcct ggcgctttga gccatggaaa
     3361 atgccgctgg acgtgcaaat gcactgcggc gtgcgcatcg gccacgacta cccggcgccc
     3421 tgcgtgccgt gggagcccac ccagcgcgcc gcgcgcgaac agctgcgcgc ctgccagccg
     3481 ccgcgcgaag cgcgcatcat caccccggct gcgccagcgc ggcgcagccg ccaccagctg
     3541 ccgccgcaac aactggcgct ggggttttag cactgcgcat aaacccctcc tggctgacct
     3601 tgccgtgtct gcacacgccc agccaggatc gctgcgcggg gtttgtgaca acgcttagcg
     3661 cccggcgcgc agttgccggt tcaacgccga cagcacggct ttcagcgctg cggcgacgat
     3721 atggctgtcc acccccacgc caaacacacc ggccgattca cccaggcgca gctccacata
     3781 gcacgccgcg cgggcatcgg cgcctgcgcc gatggcgtgt tcgtggtagt ccagcacggt
     3841 cagcggcagg cccagcgcgg cgacaaaggc gtcaatcggg ccgttgccct cgccgtgcag
     3901 cgcatacggt tcgccctggc ggcgcagggt gacgctcagc cgggtgcggc catcgttgtc
     3961 gtgttgctgg tggctggcgt aggcatacgg ggagtcgatg tccaggtatt cggcgctgaa
     4021 caatcggtgc agcgcggcgg cgtccacctc gccgccagtc gtgtcgctgt aggcttgcac
     4081 cacccggctg aattcaattt gcagccggcg cggcaaggcc aggccatagg tctgttgcag
     4141 cagataactc actccgccct tgcccgactg gctgttgacc cgaatcaccg cgtcgtagcc
     4201 gcgccgcacg tcggccgggt ctatcggcag atagggcacc gtccacgcct ggtcggcgtg
     4261 ctgctgggca aagcctttct tgatggcgtc ctgatgcgaa ccagaaaacg ccgtatacac
     4321 caaatcgccg gcatacgggt ggcgcgggtg cacgggcagt tgggtgcagt cctcgaccac
     4381 cgcgcgcacg cggtcgatgt cagaaaaatc cagccccggc gccacgccct gcgaatacag
     4441 attcatcgcc agggtgagca agtccacatt gccggtgcgc tcgccattgc caaacaaaca
     4501 gccctccacc cgctcggcgc cggccatcat cgccagctcg gccgccgcca ccgccgtgcc
     4561 acggtcgttg tgcgggtgca cgctgagaat caagctgtcg cggcgcgcca gatggcggtg
     4621 catccattcg atctggtcgg cgtaggtgtt gggggtggac agctccaccg tggccggcag
     4681 attgatgatc atcttgtgct gcggcgtcgg ctgccacact gcgctgactg catcgcagac
     4741 ttccagggcg aattccggct cgcaggcgga gaaggtttct ggcgagtatt caaacaccca
     4801 ctcggtgtcc gggcaacgcg cggtcaaggc gcgaatctgc tcggcgccgc gcaccgccag
     4861 cgccttgacg ccagcgcggt cgctattgaa cacaatgcgg cgaaacgccg gggcgatcgg
     4921 gttgtacaga tggacaatgg cgcggcgggc gccatgcagc gcttgaatgg tgcgggcaat
     4981 caaatcctcg cgcgattgcg tcagcacgct cagcgtcaca tccgccggca cggcgtcttg
     5041 ttcgatcaac tggcggacaa aatcaaaatc ggtttgcgag gccgatggaa acgccacttc
     5101 gatttccttg aagccaatct ccaccagcag ggcaaacagc tgccgcttgc gcgccgccga
     5161 catcggctcg atcagcgcct gattgccgtc gcgcaggtcg gtgctcagcc agacgggcgg
     5221 cgtgcgggtg atctggttgg gccattgccg gtcgggcaag gccagcggga caaaagccgg
     5281 gtatttgtgc tggggttggc gcaacattga gggttctcct gtggtggtgg
//
LOCUS       DAOPYX010000210         5289 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_19673_length_5289_cov_11.574895, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000210 DAOPYX010000000
VERSION     DAOPYX010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 5289)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5289)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5289
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19673_length_5289_cov_11.574895"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1025
                     /gene="mmsA"
                     /locus_tag="PKN55_10515"
     CDS             <1..1025
                     /gene="mmsA"
                     /locus_tag="PKN55_10515"
                     /EC_number="1.2.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018151063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004491 - methylmalonate-semialdehyde
                     dehydrogenase (acylating) activity [Evidence IEA];
                     GO_process: GO:0006574 - valine catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HNX71722.1"
                     /translation="LWMIPVALACGNCFVLKPSERDPSASLLLADWLAEAGLPAGVFN
                     VLHGDKTAVDALLAHPDVQAVSFVGSTPIARYIYETGAHYGKRVQALGGAKNHMVVMP
                     DADLELTVDALMGAAYGSAGERCMAISVALAVGEVGDALVAQLAERVRALKVNAGTAP
                     DAEMGPLITAEHRAKVTGYIDSGVAEGAQLVVDGRALSVPGCEQGFFLGGTLFDHVTP
                     EMTIYREEIFGPVLAVVRVASFEQALQLINDHPYGNGTSLFTRDGATARAFAHRVQAG
                     MVGVNVPIPVPMAFHSFGGWKSSLFGDQHVHGMEGVRFYTRLKTVTTRWPQRASGAEF
                     VMPTMK"
     gene            1390..2151
                     /locus_tag="PKN55_10520"
     CDS             1390..2151
                     /locus_tag="PKN55_10520"
                     /EC_number="3.1.4.17"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004114 - 3',5'-cyclic-nucleotide
                     phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-nucleotide phosphodiesterase"
                     /protein_id="HNX71723.1"
                     /translation="MKLTVLGCDGGIGGARHTTCLKLGAHALIDAGTGLGSLSLAEMA
                     AIDHVFLSHAHLDHVALLPLLVDSVGAARTRPLEVHGLPATLDSLRQHIFNWQIWPDF
                     SQLPSAEAPWLQWRPLAVGERFAMDDGYLTALPAHHTVPAVAYRLEGPTGSLVFSGDT
                     SSDEAFWQAVLASPDLRHLLIECAFPDEESRLAGQARHYSPSRLAADLAQLPAQVQVW
                     ISHLKPTDAERTLAQIAALAPCEVQALAAGVVLTV"
     gene            complement(2251..>2763)
                     /locus_tag="PKN55_10525"
     CDS             complement(2251..>2763)
                     /locus_tag="PKN55_10525"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HNX71724.1"
                     /translation="RDLDEMQALVQETLAYLRDELSGVSLELCDVNSLLDAWLADRAE
                     LGQHPLRHGAAERPLATAPAALRRIVDNLLDNAYRYGQGDVELDIVDRADSLSLRVRD
                     HGPGIAPEQLEQVFEPFYRLESSRNRSSGGSGLGLTIARHLAHQLGGQLTLENAADGG
                     LCATLTLPRR"
     assembly_gap    2764..2863
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2864..3456)
                     /locus_tag="PKN55_10530"
     CDS             complement(<2864..3456)
                     /locus_tag="PKN55_10530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX71725.1"
                     /translation="MRLLRPRSLFARLAWLLVAAVAVALAVSTWLMHRDRGEQYLRNR
                     AEFNAHRLVELANWFDTLSPAERERLAPLVSLPGLGIRLAREDAPPEALRLLPPPGIW
                     LEQALRDADLRREVRLSFGRSHHDDDEHGGRRLQAWLHLADGSWMVAKVRLTPGGHMP
                     PPPPFVGQLVATLAAVLLVALVAARSMTRPLAKLADAA"
     gene            complement(3453..4184)
                     /locus_tag="PKN55_10535"
     CDS             complement(3453..4184)
                     /locus_tag="PKN55_10535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018748188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNX71726.1"
                     /translation="MDTLDHLLVVDDDRDIRELLTDYLTRNGFRATAVADGRAMREAL
                     AAHAFDLVILDVMLPGEDGLTLCRELRARSNLPILMLTARGDDLDRIVGLEMGADDYL
                     PKPFNPRELLARIKAVLRRARALPDNLSAPSHSYARFDGWELNFDTRTLTDPAGVAVE
                     LSGAEFRLLKIFVERPHRVLNRDQLMDLLHGRDTEPFDRSIDVQVSRLRQRLGDDARE
                     ARLIKTVRGEGYVFTAPVSLAGDTA"
     gene            4527..4877
                     /locus_tag="PKN55_10540"
     CDS             4527..4877
                     /locus_tag="PKN55_10540"
                     /inference="COORDINATES: protein motif:HMM:NF019430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HNX71727.1"
                     /translation="MGHHAMMDPAKVDARLDKLKTDLKLTSAQQGAWQRYAGFVREQA
                     KQHREQFDKMREQTPPAHAPERLERMSEHMQLRMKSLEAMKEETRRFYDGLSESQKAT
                     FDQAMPQPRQGKRG"
     gene            complement(4924..>5289)
                     /locus_tag="PKN55_10545"
     CDS             complement(4924..>5289)
                     /locus_tag="PKN55_10545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX71728.1"
                     /translation="GSVSPWRHLSRVLHLVLEIVNEKATLNYIFIILILTIKINILNL
                     YQATKKALGLPEFAQYKKFPTKHTNVLFTNNYLVQMHTLSHPPSQTHDSANRQRATAQ
                     KNRRKNPPGGQYTLETSHP"
BASE COUNT          888 a         1589 c         1742 g          970 t
ORIGIN      
        1 cgctgtggat gattccggtg gcgctggctt gcggcaattg ctttgtgctc aagccttccg
       61 agcgcgaccc gtcggccagt ttgctgctgg ccgactggct ggccgaagcc gggctgccgg
      121 ccggggtgtt caatgtgctg cacggcgaca aaaccgccgt ggatgcgctg ctggcccacc
      181 cggatgtgca ggcggtgagc tttgtcggct cgacgccgat tgcccgctat atctacgaaa
      241 ccggcgcgca ctacggcaag cgcgtgcagg cgttgggcgg ggccaagaac catatggtgg
      301 tgatgccgga tgcggacctg gagctgaccg tggacgcgct gatgggcgcg gcgtatggct
      361 cggccggcga gcgctgcatg gccatttccg tggccttggc ggtgggcgag gtgggcgatg
      421 cgctggtggc gcagctggcc gagcgggtgc gtgcgttgaa ggtcaacgcc ggcacggcgc
      481 cggatgccga aatgggcccg ctgatcaccg ccgagcatcg cgccaaggtg acgggctata
      541 tcgacagcgg cgtggccgaa ggcgcgcagc tggtggtgga tggccgcgcg ctgagtgtgc
      601 cgggctgcga gcaggggttc tttttgggcg gcacgctgtt tgaccacgtg accccggaga
      661 tgaccattta ccgcgaagaa atctttggcc cggtgctggc cgtggtgcgg gtggccagct
      721 ttgagcaggc gctgcaattg atcaacgatc acccgtatgg caacggcacc agcttgttca
      781 cccgcgacgg cgccactgcg cgcgcctttg cccaccgcgt gcaggccggc atggttgggg
      841 tgaatgtgcc gattccggtg ccgatggcgt ttcacagttt tggcggctgg aagtcctctt
      901 tgtttggcga ccagcacgtc catggcatgg agggggtgcg cttttacacc cgactgaaaa
      961 ccgtcaccac ccgttggccg cagcgcgcca gcggcgcgga attcgtcatg cccaccatga
     1021 aataggccag ctggccgccg gcggcgaccg ggaagtccgc cgccgggttg cgtttccctt
     1081 gagagttggg cccgcctttg atggcgggct ttttttatgc acgatgccat cagattctcg
     1141 tctagcgtgc ggctcacgcc actcagcacc atgagcaccg ccgccaacag gatgggcaga
     1201 acacgcaatc aggccagttt ggttggccgt gcgtttctgg ccgcgcaaca caccaagccc
     1261 atccacccgc aaaccctgcc cctgaggggg cgtcggtctt gccccgcccg gcatcccccc
     1321 gcacaatact ggccacacgc tgtatcccgt gcggcgcatg atagacagac acccgaaaga
     1381 atcccctgca tgaaactcac cgtcctgggc tgcgatggcg gcatcggcgg cgcccggcat
     1441 accacctgcc tgaagctggg cgcgcacgcg ctgattgacg ccggcaccgg cctgggctcg
     1501 ctgtcgctgg cagaaatggc ggcgattgac catgtgtttc tcagtcacgc ccatctggat
     1561 catgtggcct tgctgccgct gctggtggat tcggtggggg cggcacgcac gcgcccactg
     1621 gaggtgcatg gcttgcccgc cacgctggac agcctgcgcc agcacatctt caactggcag
     1681 atctggccgg atttcagcca actgcccagc gcagaagcgc catggttgca gtggcggccg
     1741 ctggccgtgg gcgagcgctt tgccatggat gacggctatt tgaccgcact cccggcgcat
     1801 cacaccgtgc ccgctgtggc ctatcggctg gaaggcccca ccggcagcct ggtgttcagc
     1861 ggcgacacca gcagcgacga ggcgttctgg caggcggtgc tggccagccc ggatttgcgc
     1921 cacctgctga ttgaatgcgc gtttcctgat gaagagtccc ggctggctgg ccaggcccgt
     1981 cattacagcc ccagccggct ggcggccgat ctggcgcaac tgccagcaca ggtgcaagtg
     2041 tggatcagcc acctcaaacc caccgacgcc gagcgcacgc tggcgcaaat tgccgcgctg
     2101 gcgccgtgcg aagtgcaggc gctggccgcc ggagtggtgc tgacagtgta gcgccgctta
     2161 taaaccactc ttgcgttgtg gttatctgta ccatctgcgg ctgcctgcct cgccaggaac
     2221 attgtgctcg ctgagctttg tgagcgacgc tcagcgtcgg ggcagcgtca gcgtggcgca
     2281 caggccgccg tcggcggcgt tttccagcgt caattgccca cccaactggt gggcgagatg
     2341 gcgggcgatg gtcagcccca ggccgctgcc gcccgagctg cggttgcgcg agctttccag
     2401 ccgatagaag ggctcgaaca cctgttccag ttgctccggg gcaatgccgg ggccgtggtc
     2461 gcgcacgcgc aggctgaggc tgtcggcgcg gtcgacaata tccagctcga cgtcgccctg
     2521 gccatagcga taggcgttat ccagcagatt atcgacaatg cggcgcagcg ctgcgggcgc
     2581 ggtggccagc ggccgttccg ccgcgccgtg gcgcagcggg tgttggccca gctcggcgcg
     2641 gtcggccagc caggcgtcca gcaggctgtt cacatcgcac aattccaggc tgacgccgga
     2701 caattcatcg cgcagatagg ccagggtttc ttgcaccagc gcctgcattt cgtccaggtc
     2761 gcgnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnngcggcgt cggccagctt
     2881 ggccagcggc cgggtcatgc tgcgcgcggc caccagcgcc accagcagca cggcagccag
     2941 ggtggcgacc aactgtccta caaaaggagg cggtggtggc atgtggccac ctggcgtcag
     3001 acgcactttg gccaccatcc aactgccatc ggccagatgc agccaggctt gcaggcgtcg
     3061 gccgccgtgc tcgtcatcgt cgtggtggga acggccaaag ctcagccgca cctcccggcg
     3121 caggtcggcg tcacgcaggg cctgttccag ccagatgccg ggtggtggca aaagacgcag
     3181 cgcctcgggt ggggcatctt cgcgcgccag gcgaatgccc aggccgggca gcgataccag
     3241 cggcgccagc cgttcgcgct cggccgggct gagggtgtca aaccagttgg ccagctccac
     3301 cagccggtgg gcgttgaatt cggcgcggtt gcgcaggtat tgctcgcctc ggtcgcggtg
     3361 catcagccag gtgctgaccg ccagcgccac tgccaccgca gccaccaata gccaggccag
     3421 ccgggcaaac agcgagcgtg ggcgcaacag cctcatgccg tgtctccggc caggctgacc
     3481 ggcgcagtaa atacataacc ttcgccgcgc acggttttga tcagccgcgc ttcgcgggcg
     3541 tcgtcgccca ggcgttggcg caggcggctg acttgcacgt cgatgctgcg gtcaaaaggc
     3601 tcggtgtcgc ggccgtgcag caaatccatc aactggtcgc ggttgagcac gcgatgcggg
     3661 cgttcgacaa agattttcag caggcgaaat tcggcgccag acaattccac cgccacgccg
     3721 gccgggtcgg tcagcgtgcg ggtgtcaaag ttgagctccc agccgtcaaa gcgggcgtag
     3781 ctgtgtgacg gcgcggacag attatccggc agcgcgcggg cgcggcgcag caccgccttg
     3841 atgcgcgcca ggagttcgcg cgggttgaat ggcttgggca ggtagtcgtc ggcgcccatt
     3901 tccagcccga caatgcggtc caggtcgtcg ccgcgtgcgg tgagcatcaa aatcggcaga
     3961 ttggagcgcg cgcgcagttc gcggcacagg gtcaggccgt cttcgccggg cagcatcacg
     4021 tccagaatca ccagatcaaa cgcatgcgcg gccagcgcct cgcgcatggc gcggccgtcg
     4081 gccaccgccg tggcgcgaaa gccgttgcgg gtgaggtagt cggtgagcag ttcgcggatg
     4141 tcgcggtcgt catcgacgac cagcaagtga tcgagtgtgt ccatggctga attatcctgt
     4201 cttgcgggcg gcggcgggca agtttcgggt aacggcagtt aaccgcgccg gctttggcaa
     4261 cagtacgtta cattttggcc gctggcggac atgcccaggt tacatcggcg cggttaaatg
     4321 cttgcatcac gacagcggtc gtgccctgtc atcattgttg aggaggaagc atcatgttgc
     4381 ataccccatt gcgtcgcggc ctgctggccc tgggtctggt tgttggtctg gcttcggcct
     4441 cggtctgggc aatgccgcca ggtggcgatt gtggccccgg tatgagaatg ggccaccatg
     4501 gtggcgattg tggccccggt atgagaatgg gccaccatgc gatgatggat ccggccaagg
     4561 tggacgcccg gctggacaag ctcaagactg atctcaagct gacttccgcc cagcaaggcg
     4621 cctggcagcg ttacgccggt tttgtgcgcg agcaggccaa gcagcatcgc gagcagttcg
     4681 acaaaatgcg cgagcaaacc ccgccggccc acgcgccgga gcggctggag cgcatgagcg
     4741 agcatatgca gttgcgcatg aagtcgctgg aggcgatgaa agaagaaacc cgccgttttt
     4801 atgatggcct gagcgaatcg caaaaagcga ccttcgacca agccatgccg cagccccgtc
     4861 agggcaagcg cggctgatcg tctcttggcg ggcggcatgc gccgcctgcc tggggtcgaa
     4921 gcttcacggg tgtgaagttt caagggtgta ttgaccgccg ggagggttct tccggcggtt
     4981 tttttgcgct gtcgcacgct gccggttggc ggagtcgtgc gtctggctgg gggggtgaga
     5041 gagcgtatgc atttgcacaa gataattatt cgtaaataaa acattcgtat gtttcgtagg
     5101 aaatttctta tattgtgcaa attcaggtaa acctaaggct ttcttagttg cttgatataa
     5161 attcagtatg ttaatcttta tagttaatat gagtatgata aaaatataat tcaatgtcgc
     5221 tttttcgttg acgatctcaa gaaccaagtg caacactctg ctaaggtgtc gccatggact
     5281 cacactacc
//
LOCUS       DAOPYX010000211         5265 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_19797_length_5265_cov_6.909405, whole genome shotgun sequence.
ACCESSION   DAOPYX010000211 DAOPYX010000000
VERSION     DAOPYX010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 5265)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5265)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5265
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19797_length_5265_cov_6.909405"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..313)
                     /locus_tag="PKN55_10550"
     CDS             complement(<1..313)
                     /locus_tag="PKN55_10550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX71729.1"
                     /translation="MRFFRTLLAGMTLACLSACNPEALTAKISPPANPLKGDWENNGI
                     VFYRFTDTAMQAMGGGQCNYTWRSETRQSNGQQFEVVLVKCEGAAQEIPFALVDENTM
                     AF"
     gene            complement(412..816)
                     /locus_tag="PKN55_10555"
     CDS             complement(412..816)
                     /locus_tag="PKN55_10555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX71730.1"
                     /translation="MKTLLSVAALAASLLTAMPATGKTFQTYRHAGHVSSHTIGHRYG
                     SPRVRHYTATTRSDYRGRPLGITRSRITVRRQPGLYRHPRLAARPGHDRLRAHTVRHA
                     SPRLGHYSSTTRYSYRGRTVGISRSHITFRRR"
     gene            1163..3595
                     /locus_tag="PKN55_10560"
     CDS             1163..3595
                     /locus_tag="PKN55_10560"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNX71731.1"
                     /translation="MAKREKPAANRKPASRSMPVAPPPAVEEWDFSDLEDMVTLSCST
                     SDCGELDEDLLAALEPARLNPRTHATELAGLFTLRVLLRMPRLATLWGNESGKSHAYP
                     LLRPWLADDWPQRLRHRVVKQGQTSLWLDDDDMPSNADWNALLTTSAGQAVLHEALAR
                     ADLASPLSWPLPTVLAELQSRLCLSQHDMQLLCYLATVYFQDDFKSLLELAHRGYFRH
                     ARPAQFLACALDVKMMVADSWLQDERQSLARCGLVEQTDPEYASDLAEMLELSNAAKR
                     VFFGEIQCVDDLVSHIVRQASPSTLRSLDFPHMKAQLDVLLPTLAHALKLRRGNAPVR
                     KSFHALFYGSPGTGKTELARLLPSLLRQAGWPKPQLYEVPICEADGSAMDGVARFQAF
                     LLAQQLLSHTPNAVLMFDEIEDALPDHYGDRRRRSGQHKGFMNHVLETSPVPTMWISN
                     HVTHIDHAFLRRFAMPMEVPLPPAEVRQRMVDRCLKRYRVKLSDETRQRLVADEALMP
                     AMLADTARLVTSLPEQDHDAGFLRLLTNKKRLLGEDDLPDADVIEDGKAGASSALGAS
                     VACHALGKGFDPAYVNLAGSWSVDEVLTALQHQHSGRLCLYGPPGTGKTALAAHIAHT
                     LGMPLLVKSASSLLDRYVGGTEELLAKAFTEAREQNAVLFLDEADSFLLSRAGSSHRW
                     EVTQVNELLQQIDQFDGVLICATNQFAQLDAAVHRRFDFKLEFCPLTSAQALTLFCQV
                     CDCQMNDLSAEVMARLTACSTLTPGDFAVVSRRQRLLRAEWTATRWMKELEAELATRG
                     DAGASKVRLGFI"
     gene            3688..4746
                     /locus_tag="PKN55_10565"
     CDS             3688..4746
                     /locus_tag="PKN55_10565"
                     /inference="COORDINATES: protein motif:HMM:NF024676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HNX71732.1"
                     /translation="MHADDPMNNSVDDWISILEGVDQDTLDVLGHIPASPASIGHVEL
                     LKKLGRETRQGEKRKLQRHTLTMANNQLCLRQGDAGKGQSMSLCPNLRQALDLRERAH
                     NGKALVEPGNSLAAALQAEVLLAGLPDEWLVGRPETIRQLAHPADPLVAIVSETYPRL
                     SPEIMPGVFPALAEALAKNQKLELTYTLFDGRVRNQEPVSPLALLKRGPMFYLVCRKP
                     NSKQPGEFALREIALCRISRIVVLEKPAERGDFSLQAHLASNQPYFLRSDATLAIKLR
                     VFGNTRHHLSDTPLAADQLVYDGDDPDSCMLTASVMDSDMLRWWLLAMGPQVEVLEPV
                     SLREEIRRTLQQALGRYI"
     gene            complement(4876..>5265)
                     /locus_tag="PKN55_10570"
     CDS             complement(4876..>5265)
                     /locus_tag="PKN55_10570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011136666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbC family protein"
                     /protein_id="HNX71733.1"
                     /translation="EVRGQGRRQLAVFSDPDCPFCKKLEATLAQLDDVTIYTFLFPLA
                     ELHPDAPRKSAQIWCANDRAEAWTQFMRKGIAPQGAGDCDTPIAKLQTLGQQLGISGT
                     PALIFPNGQLVPGAIGKEDIEKLLGAR"
BASE COUNT         1043 a         1369 c         1657 g         1196 t
ORIGIN      
        1 caaacgccat cgtattctca tccaccagcg caaacggaat ctcctgtgca gcgccctcgc
       61 acttcaccag caccacttca aactgctggc cattggattg gcgggtttca ctgcgccagg
      121 tgtaattgca ctggccaccg cccattgcct gcatggcggt gtcggtaaag cggtaaaaca
      181 caatgccgtt attctcccaa tcgcctttca gcggattggc aggaggggaa atttttgctg
      241 tcagcgcctc ggggttgcag gcgctcaggc aggccagggt catgccggcc agcagggtgc
      301 gaaaaaaacg catagtttga tcctatcacc atcaatgatg aagttacaat atcgttgtgc
      361 gtaaagttac ggtcttggca tgctgtggca tgtgtgtgcc acggtctggg ctcagcgacg
      421 gcgaaaggtg atgtgcgagc ggctaatgcc gactgtgcgg cctcgatagc tgtagcgggt
      481 ggtgctgctg tagtggccca agcgtggtga ggcgtggcgc acggtgtggg cgcggaggcg
      541 gtcatgacca ggccgggcgg caaggcgcgg atggcgatag aggcccggct ggcgacgcac
      601 ggtgatgcgt gaacgggtaa tgcccagcgg acggccccgg tagtcgctgc gggtggtggc
      661 ggtgtagtgg cgcacacgcg ggctgccata gcgatggccg atggtgtggc tggatacatg
      721 gccagcgtgg cgataggtct ggaaggtttt gccggtggcg ggcatggccg tcagcaggct
      781 ggcggccaat gcggccacag acagcaaggt tttcatgggg gtttctcctg agtcaggccg
      841 ccgatatggc gccgttggag gcaggagtat gccgctgagg tgcgtcaacg catgtcgcgg
      901 tgcgttttgc cggcacggta tgagcattgc tggagtgagg atctaaaaaa ttttttgcct
      961 ggtgatcgaa ggtagtagaa cagcatttgt aattacgtgc gcccgcctgc gcacgtgcca
     1021 tggttttctt cctgtgccta gccccagtgg tgcgacacgg tgtgtcgcac cctgcggtta
     1081 gcatggcgtc actgatttgc tggctgttgt catggtgaca taggtcagcg agtgttcgat
     1141 gtgatgttcg gaggaatgca tcatggccaa acgagaaaag ccagcagcca acagaaagcc
     1201 agccagtcgc agcatgcctg tggctccacc ccctgccgta gaggaatggg atttttctga
     1261 tctggaagac atggtgacac tgagctgttc gacaagtgat tgtggtgagc ttgatgagga
     1321 tttgttggca gctctggagc cagccagact caatccgcgc acacatgcca ccgagttggc
     1381 cgggctgttc accctgcgcg ttctgctgcg catgcctcgg ttggccacgt tgtggggcaa
     1441 tgaaagcggc aagtctcatg cttacccgtt gctgcgccca tggctggcgg atgactggcc
     1501 gcagcgcctg cgccatcgtg tggtcaagca ggggcaaacc agtttgtggc tggacgatga
     1561 tgacatgcca agcaatgccg actggaatgc attgctgacc actagcgcag ggcaggctgt
     1621 gttgcatgag gccttggcgc gggctgatct ggcttcgcct ttgtcctggc cgctgcctac
     1681 agtgctggcg gaattgcagt cacgcttgtg cttgtctcaa catgacatgc agctgctgtg
     1741 ctacctggcg acggtgtatt ttcaggatga tttcaaatcc ttgctggagt tggcgcatcg
     1801 cggatatttc cggcatgccc ggcccgcaca atttctggcc tgcgcactgg atgtcaagat
     1861 gatggtggcg gatagctggc tgcaagatga acggcaatcg ctggcgcgtt gcggcctggt
     1921 ggaacagacc gacccggaat acgcctcgga tttggcagaa atgctggaat tgagcaatgc
     1981 cgccaagcgg gtgttttttg gcgaaattca atgtgtggat gatttggtca gccatattgt
     2041 gcgccaagcc tcgccttcta cactgcgttc gctggatttt ccgcacatga aagcccagct
     2101 tgatgtgttg ctgcccacct tggcgcacgc actgaagttg cgaagaggca atgcgcctgt
     2161 gcgcaaaagt ttccatgcct tgttttacgg ttcgcccggc acgggcaaaa cggagctggc
     2221 acggctgttg ccaagcttgc tgcgtcaagc aggttggcca aaaccgcaac tttacgaggt
     2281 gcccatttgc gaggcagatg gcagcgccat ggatggtgtg gcgcgctttc aggcattttt
     2341 gttggcccag cagttgctga gtcatacgcc caatgctgtg ctgatgtttg acgaaatcga
     2401 agatgctttg cctgatcact atggcgatcg acggcggcgc agcggccagc acaaaggttt
     2461 catgaaccat gtgctggaaa ccagcccggt gccaacgatg tggatcagca accatgttac
     2521 gcatattgac catgcgttct tacgccgatt tgccatgccg atggaagtgc cgttgccacc
     2581 ggctgaggtg cgccagcgca tggtggaccg ctgcctgaag cgatatcgag tcaagctcag
     2641 tgatgagact cgccagcgcc tggtggcgga tgaggcattg atgcctgcca tgttggcgga
     2701 taccgctcgt ttggtgacct cgctgccaga gcaagatcac gatgctggat ttttgcgact
     2761 gctgaccaat aaaaaacgcc tgcttggtga agatgacctg cctgatgccg atgtgattga
     2821 ggatggcaag gcgggtgcat cgtcagcgct tggcgcatca gtagcctgcc atgcgctggg
     2881 caaggggttt gatccagcct atgtcaattt ggcggggagc tggtcggtgg atgaagtgct
     2941 gacggcactc cagcatcagc actccgggcg tctgtgcctc tatgggcctc caggaacagg
     3001 caagactgca ttggctgcac atattgcgca taccttgggc atgccgctgc tggtaaaaag
     3061 cgcctccagc ctgctggatc gctatgtggg cggcacagaa gagctgttgg ctaaagcctt
     3121 taccgaagcc cgtgagcaaa atgccgtgtt gtttttggac gaggctgaca gctttttgct
     3181 gagccgggcc ggcagctcgc accgctggga agtgacacaa gtcaatgaat tgctgcaaca
     3241 gattgaccag tttgatggcg tgctgatttg tgccaccaac caatttgccc aattggatgc
     3301 agcagtgcat cgccgctttg atttcaaatt agagttttgc ccgctgacat cggctcaggc
     3361 attgacgcta ttttgtcagg tatgtgattg ccagatgaac gatctgtctg ccgaagtgat
     3421 ggctcgtctg acggcatgct cgactttgac gccaggagac tttgctgtag tcagccgccg
     3481 tcagcgttta ttacgtgcag aatggactgc tacacgctgg atgaaagaac tggaggcgga
     3541 gctggcgaca cgcggtgatg ccggcgcaag caaggtgcgt ctgggattta tttgagttgt
     3601 aaagctttct taacttgacg aatagtggcg ctggccagac cagcgccagt tgcctgatac
     3661 cactatcgct tcaacatgga acacactatg cacgcagacg acccgatgaa caacagtgtt
     3721 gatgactgga tcagcattct ggaaggcgtg gatcaagaca ccctggacgt gctcgggcat
     3781 attcctgcaa gcccggcatc gattggtcac gtagagttgc tgaaaaaatt ggggcgtgaa
     3841 actcgccagg gtgaaaagcg taagttacag cgccatacat tgaccatggc caacaaccaa
     3901 ctttgcttgc gtcagggcga cgccggcaaa gggcagtcga tgtctttatg cccgaatctg
     3961 cgccaggcgc tggatttgcg tgaacgggcg cacaatggca aggcgttggt ggagccaggc
     4021 aattcgttgg cggctgcgtt gcaggccgag gtgttgttgg caggtttgcc agatgaatgg
     4081 ctggtggggc ggccagaaac gattcggcaa ttggctcacc cggctgatcc gttggtggcg
     4141 attgtcagcg aaacctaccc ccgcctgtcg ccggaaatca tgccgggtgt ttttcccgcc
     4201 ttggcggagg ccttggcaaa aaaccaaaag cttgagctga cgtacacctt attcgatggg
     4261 cgtgtgcgca atcaggagcc cgtgtcaccg ttagcgttgc tcaagcgtgg gccaatgttt
     4321 tatttggtgt gccgcaaacc gaacagcaaa caaccgggcg aatttgcttt gcgcgaaatt
     4381 gccctgtgcc gtatcagccg gattgtcgtg ctggaaaaac cggctgaacg gggggatttt
     4441 tccttgcagg cgcatctggc cagcaatcag ccttattttt tgcgttcgga tgccacgctg
     4501 gccatcaagc tacgggtgtt tggtaatacc cgccatcatt tgtctgacac tccgctggcg
     4561 gcagatcagc tggtatacga tggtgatgac ccggattcat gcatgctgac cgcatcggtg
     4621 atggattctg acatgttgcg ttggtggctg ctggccatgg ggccacaggt ggaagtgctg
     4681 gagccagtga gcctgcgcga ggaaatccgc cgcacgctgc aacaggcctt gggacgttat
     4741 atttgagagc ctgccgattt ttttcaaaat caagcctata ggcttcctac tttcgcaggt
     4801 gctattccta aatgacggta aaggcagcac accgcccgtg ccatgcaggg gggacatccc
     4861 cccgcacgtt accccctacc tcgcccccag cagcttctca atatcctcct tgccaatcgc
     4921 ccccggcacc agttggccgt tcgggaagat cagtgccggg gtgccgctga tgcccagttg
     4981 ctggcccagg gtttgcagtt tggcaatcgg ggtgtcgcag tcgcctgcgc cttgcggggc
     5041 gatgcccttg cgcatgaatt gtgtccaggc ttctgcgcgg tcgttggcgc accagatttg
     5101 cgcggatttg cgcggggcgt cggggtgcag ttcagccagc gggaacagga aggtatagat
     5161 cgtcacgtcg tccagctggg ccagggtggc ttccagtttt ttgcagaacg ggcaatccgg
     5221 gtcggaaaac accgccagct ggcgacggcc ctggccgcgc acttc
//
LOCUS       DAOPYX010000212         5247 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_19900_length_5247_cov_10.665254, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000212 DAOPYX010000000
VERSION     DAOPYX010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 5247)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5247)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5247
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19900_length_5247_cov_10.665254"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1580
                     /locus_tag="PKN55_10575"
     CDS             <1..1580
                     /locus_tag="PKN55_10575"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNX71734.1"
                     /translation="IAVMTALQIIVAAVAYFDLQRVLDNDQREHAVVQVLKHNMTEAR
                     FHVVQVQQFLTDASATGERDGLKDASEHFQALGKNLRAIAEFDTAFRGETDKITALAQ
                     NYYQTGTTMAEAYITQGREAGNALMKAPGSGFDERALALTHQLEAMARQVDVRVAESE
                     AATEATVQRLRATVIGLSVFLALTLLLGGIWLYRQVFGMLGGEPARAVRLAQRIAQGD
                     LSRKIEADDAPDSLLGALSDMRSQLCHVTQHIQTLAVQLDDGAQALASTSCHMEQGAQ
                     TQSDATRAMAAAMEEIQHSITQLGEQSGQVGDEAHQAGDMVAACEALEHASADSVRAM
                     AGQFQHAAGVITDLHQQTDAIAQLTQTIHDIADQTNLLALNAAIEAARAGEQGRGFAV
                     VADEVRKLAERTAQTTLDITSQTSALAQRMSEVVGTMENGVTASDQGVARTGEVSQAI
                     GHIRQNTEQINQHIHGVALALREQEHAMAELGQRLEVIVSMSDTHHHAIESTASAASQ
                     LTANAHSLAEAVGHFRV"
     gene            2012..3832
                     /locus_tag="PKN55_10580"
     CDS             2012..3832
                     /locus_tag="PKN55_10580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014086713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cache 3/Cache 2 fusion domain-containing
                     protein"
                     /protein_id="HNX71735.1"
                     /translation="MKGLGLQARFALFGFAPLVAALVIGSLLLSIKQSRQLEDEVRER
                     SQATAAGVIQLLQMSDSLIGEQTRSAMKLFKEKSAQQGPASAGEAVAVGSRRPLGLRF
                     GGRAQTLDTALVDSVTAIAGGSATLFAREGQDFVRIATNVKTAEGQRAVGTVLDPQSQ
                     AYAMLSKGQPFYGQVDILGAPYLTGYEPILENGKVIGAWYVGFKADLAGLKTLIGQVS
                     AFGSGFVVLLDGKGRPFLHSQSASAERIQTQLQQARQGEGEWRVIEQPFPAWGFAVAV
                     AYSEDEVAAAGRRAALPVLLSGLGITIALLALLMLVLRRVVLAPIQQAVTAANALADG
                     DLTVRVHAERDDEIGRLLAAMAHMVERLADIIGQVRGAADNLSSASVEVSATAQSLSQ
                     AASQQASSVEEISSTLEQASASVSNNTQNARATETMAMDAAREAEAGGEAVSGNVRAM
                     QQIAGKVGIIDDIAYQTNLLALNAAIEAARAGEHGKGFAVVAAEVRKLAERSQVAAGE
                     IGAVAGDTVRQAERAGELLHRIVPAIGRTADLVREIAAASDEQSAGIGQINQAMGQLN
                     QATQQNAAASEQLAATSEELGAQAESLQQLMHFFRVENAR"
     gene            complement(3829..5151)
                     /locus_tag="PKN55_10585"
     CDS             complement(3829..5151)
                     /locus_tag="PKN55_10585"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HNX71736.1"
                     /translation="MLAPRRHTAALWLTLILLIVWSIPHTVALRYTLMTGLALCVFSV
                     PAVRQAVAVQWRSQPALWIYLALSAWLLIQIPTVAMHPQETLEELRGQWLKSSLALLL
                     GMGLMQLARTTPGSSRAVVFNRVFVALGLAVWIQGADYLWAWLVLGEPSFNSTGVFNG
                     KVQVSYLANLFLSLCMAEAFSRVCGGGRYVAVSSPLLAAWALCAVAVTFFSGARNGLI
                     GVVLLCVSTGLLMLYYAPSRIRPWQRLGGFALLLCVAAGLAGAQLKTDTRWARFIEST
                     SYSFQTDTVLSWLDPKRYPYPILSDGQPLDDSAYERTAWIIVGMKLSAEHKLGVGFTR
                     KAYTNTVMDKYGVLVRHSHSGLVEYTLGMGWPGIALWAAFLLTVFSQGLRRLLRKHDT
                     LALASMFLVSGFAGRMIIENVTRDHMMEMFMFLIGLMLAALVRPAQAR"
BASE COUNT         1095 a         1689 c         1631 g          832 t
ORIGIN      
        1 ttattgccgt gatgactgcc ttgcagatca tcgtggctgc cgtggcgtat ttcgacctgc
       61 aacgtgtgct ggacaatgac cagcgcgagc acgcggtggt gcaggtgctc aagcacaata
      121 tgaccgaagc ccgctttcat gtggtgcagg tgcagcagtt tctgaccgat gccagcgcca
      181 ccggcgagcg tgacgggctg aaagatgcca gcgagcattt tcaggcgctg ggcaaaaatc
      241 tgcgcgccat tgcagaattc gacaccgcct ttcgcggcga aaccgacaaa ataactgcgc
      301 tggcgcaaaa ctactaccaa accggcacca ccatggccga ggcatacatc acccagggcc
      361 gtgaggccgg caatgcgctg atgaaagccc ctggcagcgg ctttgacgag cgtgcactgg
      421 cgctcactca ccagttggaa gccatggccc gacaagtcga tgtccgcgtg gcagaatccg
      481 aggcagccac ggaggccacc gtgcagcgtc tgcgcgccac cgtcattggc ctgagcgtgt
      541 ttctggcctt gaccctgctg ctgggcggca tctggctgta ccgccaggtg ttcggcatgc
      601 tgggtggcga gccggcccgc gccgtgcgcc tggcccagcg cattgcccag ggcgatctga
      661 gccgcaagat cgaagccgac gatgcgcccg acagtctgct gggtgcgctc agcgacatgc
      721 gcagccagtt gtgtcatgtc acccagcaca tccagacgct ggcggtgcaa ctggatgacg
      781 gcgcccaggc gctggccagc acctcgtgcc acatggaaca aggcgcccaa acccaaagcg
      841 acgccacccg cgccatggcc gcagccatgg aagaaattca gcacagcatc acccaattgg
      901 gcgagcaaag cgggcaggtc ggcgacgagg cccaccaggc tggcgatatg gtggccgcct
      961 gtgaagcact ggagcacgcc agcgccgata gcgtgcgagc catggcgggc cagtttcagc
     1021 atgcggccgg ggtgattacc gacctgcatc agcaaaccga cgccattgcc caactgacgc
     1081 aaaccattca tgacattgcc gaccagacca atctgctggc cttgaacgcc gccattgaag
     1141 ccgcgcgcgc cggcgagcag ggtcgcggct ttgccgtggt ggccgacgaa gtgcgcaagc
     1201 tggccgaacg taccgcccaa accacgctgg acatcactag ccagaccagc gccctggccc
     1261 agcgcatgag cgaagtggtc ggcaccatgg aaaacggcgt gacggccagt gatcagggcg
     1321 tggcacgcac cggcgaggtc agccaggcca ttggccatat ccgccagaac accgagcaga
     1381 tcaatcagca tatccatggc gtggcgctgg ccctgcgcga gcaggaacac gccatggccg
     1441 agctgggcca acggctggag gtgatcgtca gcatgagcga cacccaccac cacgccatcg
     1501 aaagcaccgc cagcgcggcc agccaactga ccgccaatgc ccattcgctg gcggaagccg
     1561 tggggcattt ccgcgtctga tccagatcaa ctctgttgcg gaggtggcca cagtcgcctg
     1621 gtggccggcc acctccgcaa cagcgcatgc catacgggca aagtgcacaa acagcccagt
     1681 gacgccagtc atgccagtgg cttgtaaaga ccagcccctt aaggcaacgc cagccaatta
     1741 acccttgtca aagaatggtt ttttctacat ggccaaccgg cttatgtaga taggccacgc
     1801 aaacccgccg atcctggcta aaccgcaaac atatcaccca tggatatttc ccggcatgtc
     1861 accgtgccag gcatggtcat ttacagtatt tcttaataat cattcccact ttccctgctg
     1921 agcaattgtt gttagcgtgt ctgctgcgca gcccattgcc caacccggct ggctgctgcg
     1981 ccatgtcgtg atgcccccat aaggaagcaa gatgaaaggt ctgggacttc aagcccgttt
     2041 cgccctgttc gggtttgccc cgctggtggc cgccctggtc attggctcgt tgttattgtc
     2101 gatcaagcaa tcccgccagc tggaagacga agtgcgcgag cgctcgcaag ccaccgctgc
     2161 cggggtgatt cagctgctgc aaatgagcga cagcctgatc ggcgagcaaa cccgctcggc
     2221 gatgaaatta ttcaaggaaa aaagcgccca gcaaggcccg gccagcgcgg gcgaggcggt
     2281 ggccgtcggc agccgtcgcc cgctgggcct gcgttttggc gggcgcgcgc aaacgctgga
     2341 caccgccctg gtggacagcg tcaccgccat tgccggcggc agcgccacgc tgtttgcccg
     2401 cgaaggccag gattttgtcc gcatcgccac caatgtcaaa accgccgaag gccagcgcgc
     2461 agtgggcacc gtactggacc cgcaaagcca ggcttacgcc atgctcagca agggccagcc
     2521 gttttatggc caggtggata ttctgggcgc gccctatctg accggctacg agcctatcct
     2581 ggaaaacggc aaggtgattg gcgcctggta tgtcggtttc aaggctgatc tggccgggct
     2641 gaaaaccctg attggccagg tcagcgcctt tggctcgggc tttgttgtgc tgctggatgg
     2701 caagggccgg ccgtttttgc attcgcaatc cgccagcgcc gagcgcattc agacgcagct
     2761 gcaacaagcg cgtcagggcg agggcgaatg gcgcgtcatc gagcagccgt ttccggcctg
     2821 ggggtttgcc gtggcggtgg cgtatagcga agacgaagta gccgctgccg gccgccgcgc
     2881 cgcactgccg gtgctgctgt ccgggctggg catcaccatc gccctgctgg cgctgctgat
     2941 gctggtgctg cgccgggtgg tgctggcgcc gattcagcag gcggtcactg ccgccaatgc
     3001 gctggccgac ggcgatttga ccgtgcgcgt gcacgccgag cgcgacgatg aaatcggccg
     3061 cctgctggcc gccatggcgc atatggtcga gcggttggcc gacatcatcg gccaggtgcg
     3121 cggcgcggcg gacaatctgt cgtcggcgtc ggtagaagtc tcggctaccg cgcaaagcct
     3181 gtcgcaggcc gccagccagc aggcctcgtc ggtggaagaa atctccagca cgctggaaca
     3241 ggccagcgcc tcggtgagca ataacacgca aaacgcccgc gccaccgaaa ccatggcgat
     3301 ggatgccgcc cgcgaagccg aagctggcgg cgaggcggtc agcggcaatg tgcgcgccat
     3361 gcagcagatt gccggcaagg tgggcatcat cgacgatatt gcctaccaga ccaatctatt
     3421 ggccttgaac gcagcgattg aggccgcccg cgctggcgag catggcaagg gctttgccgt
     3481 ggtggcggcg gaggtgcgca agctggccga gcgcagccag gtggcggccg gagaaattgg
     3541 cgcggtggcc ggcgacaccg tgcgccaggc cgagcgcgct ggcgagttgc tgcaccggat
     3601 tgtgccggcg attggccgca cggcggactt ggtgcgcgaa atcgccgccg cctcggatga
     3661 gcaatccgcc ggcattggcc agatcaatca ggcgatgggg cagctgaacc aggccaccca
     3721 gcaaaacgcg gcggcgtcgg agcaactggc cgccacctcg gaagaactgg gcgcacaagc
     3781 cgaatcactg caacagctga tgcatttttt tcgggtagag aacgcccgtt aacgcgcttg
     3841 agctggccgc accagcgcgg ccagcatcag cccgatcaaa aacatgaaca tttccatcat
     3901 gtgatcgcgg gtgacgtttt cgatgatcat gcgcccggca aagccactga ccagaaacat
     3961 gctggccagc gccagcgtgt cgtgtttgcg cagcagccgg cgcaggccct ggctgaacac
     4021 cgtcagcaaa aacgccgccc acagcgcaat gccaggccag cccatgccca gggtgtattc
     4081 caccagaccg ctgtgtgaat ggcgcaccag cacgccgtat ttgtccatca ccgtgttggt
     4141 gtacgccttg cgggtaaagc ccacgcccag cttgtgttcg gcagacagct tcatgccgac
     4201 gatgatccag gcggtgcgtt cgtaggcgga atcatccagc ggctggccat cggacaaaat
     4261 cggatacggg tagcgcttgg ggtccagcca actcagcacg gtgtcggtct gaaaagaata
     4321 cgatgtggat tcgataaagc gcgcccagcg cgtgtcggtt ttcagctgcg cacccgccag
     4381 gccggccgcc acacacagca gcaaggcaaa accgcccagc cgttgccacg ggcgaatgcg
     4441 cgacggcgcg taatacagca tcagcaagcc ggtggagaca cacagcaata ccacgccaat
     4501 caggccattg cgcgcaccgg aaaaaaacgt caccgccacc gcacacaaag cccacgctgc
     4561 caacaagggc gaagacactg ccacatagcg cccgccgccg cacacccggc tgaacgcctc
     4621 ggccatgcac agcgacaaaa acagattggc cagatacgac acctgcacct tgccgttgaa
     4681 aaccccggtc gagttaaacg acggctcgcc cagcaccagc caggcccaca gatagtccgc
     4741 cccctgaatc cacaccgcca gacccagcgc cacaaacacc cggttgaaca ccaccgcgcg
     4801 cgagctgccc ggtgtggtgc gtgccagttg catcaggccc atgcccagca gcaaggccag
     4861 cgagcttttc agccactggc cgcgcagctc ttccagggtt tcttgggggt gcatggccac
     4921 ggtgggaatc tgaatcagca accaggcgga cagcgccaaa taaatccaca aagcgggttg
     4981 gctgcgccac tgcaccgcca ccgcctgacg caccgccggc acactgaaca cacacaaggc
     5041 cagcccggtc atcagcgtat agcgcaaggc cacggtgtgc ggaatcgacc agacaatcaa
     5101 taaaatcagc gtcagccaaa gggcggcggt atggcggcgg ggggcgagca tcagacggga
     5161 gcctttcggt cggcgtcggc atccagcagc gacagcgcgg cgtcggcaat ctgctgggcg
     5221 gaaatcgatt caatacaggc gcgcgtg
//
LOCUS       DAOPYX010000213         5235 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_19960_length_5235_cov_12.413320, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000213 DAOPYX010000000
VERSION     DAOPYX010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 5235)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5235)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5235
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19960_length_5235_cov_12.413320"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            52..1140
                     /gene="aroG"
                     /locus_tag="PKN55_10590"
     CDS             52..1140
                     /gene="aroG"
                     /locus_tag="PKN55_10590"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HNX71737.1"
                     /translation="MQRQTDDVRIREIKELLPPIAHLYELPITDSAAELIYATRSDIA
                     RLLRGEDDRLLVVIGPCSIHDTQAALEYAARLQPLRDALAGELLVVMRVYFEKPRTTV
                     GWKGLINDPELNGTYDINRGLRMARKLLLDLNNQGMPAATEFLDMITPQYVADLISWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGNLKIAVDAIRAASQPHHFLSVTKAGHS
                     AIVGTGGNPDCHVILRGGKEPNYASEHVRGAAAELAAVGLPQKVMVDFSHANSRKDYK
                     RQMEVATDVASQMAAGDNHIFGVMVESHLVEGRQDLKPGCQPVYGQSITDACIGWADT
                     EALLTTLADGVKARRAKLGQPEDASADE"
     gene            complement(1330..2553)
                     /locus_tag="PKN55_10595"
     CDS             complement(1330..2553)
                     /locus_tag="PKN55_10595"
                     /inference="COORDINATES: protein motif:HMM:NF019421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HipA family
                     toxin"
                     /protein_id="HNX71738.1"
                     /translation="MRQVNVVYCGWGEQWPLGRLADDGRTLLFEYAPEALAQGLELSP
                     LHLKLRPQAYGDFPVYQHRLPGLLADSLPDGWGLLLMDRVFRQQGLRHPGPLDRLAFI
                     GHRAMGALCFIPAQDADAQAPDWGLLALAQESARVLAGEAGAALRELALTGGSPQGAR
                     PKALVQYDMASGQISTRPDAAGSPWLVKFPAQGEHQEVCAIEQCYAELARECGLETPE
                     SRWFEVSAKLAAFGAARFDQEAGLRVPVHSLAGLLHIDFRLPGAADYTTFLRATRLLT
                     RDEREVEKAYDRAVFNVLFHNRDDHPKNLAWRLGRDRHWRLAPAFDLTFSTGPMGQHH
                     MDVCGEGAAIERGHLLRLAQEGGIGIHTAKHSIDRMLPHAASLAGRLADFPVRRATAQ
                     VLCATVQACYRRLMG"
     gene            complement(2541..2861)
                     /locus_tag="PKN55_10600"
     CDS             complement(2541..2861)
                     /locus_tag="PKN55_10600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017936956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNX71739.1"
                     /translation="MSFILSLPNEILCELGARLRMQRLAQSLSQRELAHMAGLSLGAL
                     RKLETDGQCSLETLIRAVQALGLSTELDELFVLKRQSIAQMAQAEAAQQRQRAPRRKS
                     SCDR"
     gene            complement(2959..3708)
                     /locus_tag="PKN55_10605"
     CDS             complement(2959..3708)
                     /locus_tag="PKN55_10605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014088762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00266 family protein"
                     /protein_id="HNX71740.1"
                     /translation="MTMHVIDYEIIGDDLQFVEVELDPGEAAVGEAGAMMYMQDGVSM
                     DTVFGDGSAQEGGWMGKLFGAGKRLLTGESMFTTVFVNEGQGKKRVAFAAASPGKIVP
                     VHLRELGGALLAQKDAFLCAAKGVSLGIAFQRRLGAGLFGGEGFILQKLEGDGYVFLH
                     AGGALVEKQLKAGEVLRVDTGCVVAFQPSVDFDIEYVGKLKSALFGGEGLFFATLTGP
                     GHVWLQSLPFSRLADRILSQAPAATGSKSSD"
     gene            complement(3705..4295)
                     /gene="mobA"
                     /locus_tag="PKN55_10610"
     CDS             complement(3705..4295)
                     /gene="mobA"
                     /locus_tag="PKN55_10610"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014088761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HNX71741.1"
                     /translation="MAISYTALLLAGGQARRMGGVDKGLQTLHGQPLAAHVLASLAAQ
                     SQPPARCLISANRHLDAYAALGWPVLPDAEPDYPGPLAGIAAGLAALQDDWLLVAPCD
                     AIRLPADLAARLLSAAAGRDAASAADPEHWHPTLLALHRRVAPSLSAWRQGGGRSIRG
                     WLAGLDHQECQFAVPFTNANTLDDLARLAAQQGDCA"
     gene            complement(4286..4744)
                     /locus_tag="PKN55_10615"
     CDS             complement(4286..4744)
                     /locus_tag="PKN55_10615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HNX71742.1"
                     /translation="MSAAFTVAVQTARFDPGAEMAQLAGLPDVGAQVCFVGQVRDFGD
                     SQAVTALELEHYPGMTEKSLLAILDQARARWPLAAARVVHRVGYLPLGEAIVLVLTAS
                     AHRGDAFAACEFIMDYLKRDAPFWKKEHTAAGSHWVEAKHSDQQAAQRWQ"
     gene            complement(4747..5004)
                     /gene="moaD"
                     /locus_tag="PKN55_10620"
     CDS             complement(4747..5004)
                     /gene="moaD"
                     /locus_tag="PKN55_10620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018150034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin converting factor subunit 1"
                     /protein_id="HNX71743.1"
                     /translation="MTTLNLLYFARLRDALGCGQETLTLDGAATVASLLDALRARGGV
                     WAEQLDAARSFRVAVNQTVAHAGQALADGDEVAIFPPVTGG"
     gene            complement(5006..>5235)
                     /locus_tag="PKN55_10625"
     CDS             complement(5006..>5235)
                     /locus_tag="PKN55_10625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX71744.1"
                     /translation="PPDGLAQAASSRARPNSLPSPISRKRFTGHSTMLSWEEWIFVGI
                     MAAVPIGGAIFHIWVMLLGHGKKHADSSKDQ"
BASE COUNT          952 a         1755 c         1668 g          860 t
ORIGIN      
        1 gtcaaaccac gctaggcttg gcgttttgcc atcgttttat tagagtgcgc catgcaacgc
       61 caaactgacg acgtccgtat ccgggaaatc aaagaacttc tccccccgat tgcccacctc
      121 tacgaactgc ccatcacgga ttccgccgcc gagctgatct acgccacccg cagcgacatc
      181 gcccgcctgc tgcgcggcga agacgaccgc ctgctggtgg tgattggccc gtgctcgatc
      241 cacgacaccc aggccgccct ggagtatgcc gcccgcctgc aaccgctgcg cgatgcgctg
      301 gccggtgagc tgctggtggt gatgcgcgtg tattttgaaa aaccgcgcac cacggtgggc
      361 tggaagggcc tgatcaacga ccccgagctg aacggcacct acgacatcaa ccgtggcctg
      421 cgcatggcgc gcaaactgct gctggacctg aacaaccagg gcatgccggc cgccaccgaa
      481 ttcctggaca tgatcacccc gcaatacgtg gccgacctga tcagctgggg cgccatcggc
      541 gcgcgcacca ccgaaagcca ggtgcaccgc gagctggcct ctggcctgtc ctgcccggtg
      601 ggcttcaaga acggcaccga cggcaacctg aaaatcgccg tggacgccat ccgcgccgcc
      661 agccagccgc accacttcct gtcggtgacc aaggccggcc attccgccat tgtcggcacc
      721 ggcggcaacc cggactgcca cgtgatcctg cgcggcggca aagagccgaa ctacgccagc
      781 gagcatgtgc gcggcgccgc cgccgaactg gccgccgtgg gcctgccgca aaaagtgatg
      841 gtggacttca gccacgccaa cagccgcaaa gactacaagc gccagatgga agtcgccacc
      901 gacgtggcca gccagatggc cgccggtgac aatcacatct ttggcgtgat ggtggaaagc
      961 catctggtgg aaggccgcca ggacctcaag ccgggctgtc aaccggtgta cggccaaagc
     1021 atcaccgacg cctgcatcgg ctgggccgac accgaagccc tgctcaccac cctggccgac
     1081 ggcgtcaaag cccgccgcgc caagctgggc cagccggaag acgcctcggc agacgaataa
     1141 accgcgtccg cgccccactc gcacgcacga tggcaaaaca cgcgcgttga ccagtccggc
     1201 cgacatggct gactgaaggg aaatacacat gcccggtgat gatctcaccg ggcattgtgt
     1261 tgtggggggc ctcaaacacc aagccccaca tcgtgaatcc ggacaagcat cgccgccgtc
     1321 aaccctacct caccccatca gccgccgata gcaagcctgt accgtcgcgc ataacacctg
     1381 cgcagtcgcc cgccgcacgg gaaaatccgc cagccgtccg gccaggctgg cggcatgtgg
     1441 caacatgcgg tcaatactgt gtttggcagt gtgtatgccg atgccgccct cctgggccaa
     1501 gcgcaatagg tggccgcgtt cgatggccgc gccctcgcca cagacatcca tatgatgctg
     1561 gcccatcggg ccagtgctaa aagtcaggtc aaatgcgggc gccaagcgcc agtgtcggtc
     1621 gcggccaaga cgccaagcca gatttttggg gtggtcgtcg cggttatgga acagcacatt
     1681 gaagacggcc cgatcatagg ctttttccac ttcacgttcg tcacgcgtca acaggcgggt
     1741 cgcgcgcaga aaagtggtgt agtcagccgc gcctggcaga cgaaagtcga tatgcagcaa
     1801 gccggcgagg ctatgcacag gcacgcgcaa gccggcttct tggtcaaaac gcgccgcgcc
     1861 aaacgccgcc aatttggccg acacttcaaa ccagcggctc tcgggggttt ccaagccaca
     1921 ctcacgcgcc agttcggcat aacactgctc aatggcgcag acttcctgat gctcgccctg
     1981 ggcgggaaac ttaaccagcc aggggctgcc agccgcatct gggcgggtgc tgatttgccc
     2041 actggccatg tcatattgca ccagcgcctt gggccgcgcg ccttgtggcg agcccccagt
     2101 caaggccagt tcgcgcaagg ctgcgcccgc ctcacccgcc aggacgcgcg cgctttcctg
     2161 ggcgagcgcc agcaggcccc aatccggcgc ttgggcatcg gcgtcttggg ccgggataaa
     2221 acacaaggcg cccatggccc ggtggccaat gaacgccagt cgatccagcg ggccagggtg
     2281 gcgcagcccc tgctgacgaa agacacgatc catcagcaat aaaccccagc catccggcaa
     2341 actatcggcc agcagccccg gcagacgatg ctgatatacc ggaaaatcgc catacgcctg
     2401 ggggcgcagt ttcaggtgca gcggcgacag ctccagtcct tgggccaagg cctcaggggc
     2461 atattcgaat agcagcgtac gaccatcatc cgccagccgc cccaacggcc actgctcccc
     2521 ccacccgcaa tacaccacat tcacctgtcg catgacgatt tcctgcgtgg cgcgcgttgg
     2581 cgttgctggg cggcttcggc ttgcgccatc tgggcgatgg attggcgttt cagcacaaac
     2641 agctcgtcca gctcggtgct caggcccagc gcctgcaccg cccgaatcag cgtttccagc
     2701 gagcattggc cgtcggtttc cagtttgcgc agcgcgccca gcgaaagccc ggccatgtgc
     2761 gcgagttcgc gttgtgacag cgactgggcc aggcgttgca tccgcaggcg ggcaccgagt
     2821 tcgcacagaa tttcattggg gagagataag ataaaagaca ttttgctgtg acttaagccc
     2881 ggattgacca gttaatcacc attataatgt gttttatctg gtggggcggc ggtgttgacc
     2941 gccaccccgc cacaagcctt aatccgacga cttactcccc gtcgccgctg gcgcttggct
     3001 caaaatccgg tcggccagcc gcgagaacgg cagcgattgc agccagacgt gccccggccc
     3061 ggtcagggtg gcgaaaaaca gcccttcgcc gccaaacagc gcgcttttca gtttgccgac
     3121 gtattcgatg tcaaagtcca cgctgggctg aaacgccacc acgcagccgg tgtccacgcg
     3181 cagcacttcg ccggctttca gttgtttttc caccagcgcg ccgccggcgt gcagaaacac
     3241 atagccgtcg ccttccagtt tttgcaggat aaagccctcg ccgccaaaca ggccggcgcc
     3301 cagccggcgc tgaaaggcga tgcccagcga cacgcccttg gcggcgcaca gaaaggcgtc
     3361 tttttgcgcc agcagcgcgc cgccgagttc gcgcaggtgc accggcacga ttttgcccgg
     3421 cgaggcggcg gcaaaggcca cgcgcttttt gccctggcct tcgttgacaa acacggtggt
     3481 gaacatcgat tcgccggtga gcaggcgctt gccggcgcca aacagcttgc ccatccagcc
     3541 gccttcttgc gccgagccgt cgccgaacac ggtgtccatg ctgacgccgt cctgcatata
     3601 catcatggcg ccggcttcgc cgacggcggc ttcgccgggg tcgagctcca cttcgacaaa
     3661 ctgcaagtcg tcgccgatga tttcgtagtc aatcacgtgc atggtcatgc gcagtctcct
     3721 tgttgggcgg ccaggcgggc caggtcgtcc agggtgttgg cgttggtgaa gggaacagca
     3781 aactgacatt cctggtgatc cagcccggcc agccagccgc gaatgctgcg cccgccgccc
     3841 tgacgccagg cgctcaagct gggggcgacg cggcggtgca gcgccagcag ggtggggtgc
     3901 cagtgttcgg ggtcggcggc gctggcggcg tcgcggccgg cggcggcgct cagcaggcgc
     3961 gcggccaggt cggccggcag gcggatggcg tcgcagggcg caaccagcag ccagtcgtct
     4021 tgcagggcgg ccaggccggc ggcgatgccg gccagcgggc cggggtagtc gggttcggcg
     4081 tcgggcagca ccggccagcc cagcgcggcg taggcgtcca ggtggcggtt ggcgctgatc
     4141 aggcagcgcg ccggcggctg gctttgcgcg gccaggctgg ccagcacatg cgcggccagt
     4201 ggttggccgt gcagggtttg caggcctttg tccacgccgc ccatgcggcg ggcctggccg
     4261 ccggccagca gcagcgcggt gtagcttatt gccaccgttg cgccgcctgt tggtcgctgt
     4321 gcttggcttc cacccagtgg ctgccggcgg cggtgtgttc ttttttccag aacggcgcgt
     4381 cgcgcttgag gtagtccatg atgaattcgc aggcggcaaa ggcgtcgccg cgatgggcgc
     4441 tggcggtgag caccagcaca atggcttcgc ccagcggcag atagcccacc cggtgcacca
     4501 cccgcgccgc cgccagcggc cagcgcgcgc gcgcttggtc gaggatggcc agcagggatt
     4561 tttcggtcat gccggggtag tgttccagct ccagcgcggt gaccgcctgg ctgtcgccaa
     4621 agtcgcgcac ctggccaaca aagcacactt gcgcgccgac atcgggcagg ccggccagct
     4681 gggccatctc ggcgccgggg tcaaagcgcg cggtttgcac ggcgacggta aaggcggcgc
     4741 tcatgttcag cccccggtga ccggcgggaa aatcgccact tcgtcgccgt cggccagcgc
     4801 ttggccggcg tgggcgacag tctggttgac cgccacgcga aagctgcgcg cggcgtccag
     4861 ttgttcggcc cacacgccgc cgcgcgcgcg cagcgcatcc agcaggctgg ccaccgtggc
     4921 ggcgccgtcc agcgtgaggg tttcctggcc gcagcccagc gcgtcgcgca ggcgggcgaa
     4981 gtacagcaga ttgagggtgg tcacgttatt ggtcttttga tgaatcggcg tgcttcttgc
     5041 cgtggccaag cagcatcacc cagatatgaa aaatcgcgcc gccaatcggc acggcggcca
     5101 tgatgccgac aaaaatccat tcttcccagc tgagcatggt ggagtgtcct gtaaagcgct
     5161 tgcgcgatat gggtgatggt aaggaattcg gccgcgcgcg gctactggcg gcttgggcta
     5221 ggccatcggg tgggg
//
LOCUS       DAOPYX010000214         5224 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_20015_length_5224_cov_10.203521, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000214 DAOPYX010000000
VERSION     DAOPYX010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 5224)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5224)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5224
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20015_length_5224_cov_10.203521"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..256)
                     /locus_tag="PKN55_10630"
     CDS             complement(<1..256)
                     /locus_tag="PKN55_10630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018152625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNX71745.1"
                     /translation="MQDALTVLQQVFGYPEFRGLQAEIVAHVATGHHALVLMPTGGGK
                     SLCFQVPALLRPGVTVVVSPLIALMEDQVATLSELGVAAAY"
     gene            502..954
                     /locus_tag="PKN55_10635"
     CDS             502..954
                     /locus_tag="PKN55_10635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012698215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX71746.1"
                     /translation="MSSRALVAALLAVGLAACATQEDPGRSAPRDPGAATQWQNLGVT
                     PNGNILHELDKQSIVRHGDVVTFRDRKTIFNLKRETFTSLPRHKVSVNSWRIDCQAQT
                     YQLLASSLFDENGKPVASYTYNDGEIRPAAITPNSASHQQMQAVCRRP"
     gene            978..1757
                     /locus_tag="PKN55_10640"
     CDS             978..1757
                     /locus_tag="PKN55_10640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HNX71747.1"
                     /translation="MTAIKRVVFNQKGGVGKSTITANLAAVAAQQGRRALVVDLDPQG
                     NLSHYVYGPDADQLRPSVFDFFDQILNFSLNSQPTLAHVHATPFAGLSLMCSHPELGE
                     LGPKLEARYKMYKLRDALNELAKEFDEIWVDTPPALNFFSRSALIACERVLIPFDCDD
                     FSRRALYTLLDNLQEIRGDHNAGLQLEGIIVNQFQPRAALPAKLVAELAAEGLPMLNS
                     KLSSSVKIRESHQSSQPMVYLDPKHKLSQEFVALYQELSGR"
     assembly_gap    1758..1857
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1858..2531)
                     /locus_tag="PKN55_10645"
     CDS             complement(<1858..2531)
                     /locus_tag="PKN55_10645"
                     /inference="COORDINATES: protein motif:HMM:NF015438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HNX71748.1"
                     /translation="MRAVNWPPPLAIAAPRIGQARPLGPNGRLSAIDKHAVHGPLWLD
                     ALGLRGDEQADRRVHGGVDKALHYFPAEHYRSLAAEDPGLRLAVGDFGENLSGLGLLE
                     QDVCLGDVFALGPCRVQLSQGRQPCWKLNVRFARPDMARRVQDSGRVGWYFRVLQPGW
                     LQAGDVLQLLARPWPDSQLARVLRALNQANVAADERLAVAALPGVPPRWRERLLAQAA
                     HGHREDA"
     gene            complement(2519..3229)
                     /locus_tag="PKN55_10650"
     CDS             complement(2519..3229)
                     /locus_tag="PKN55_10650"
                     /inference="COORDINATES: protein motif:HMM:NF005458.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HNX71749.1"
                     /translation="MSGRVLAIRHVQFEDLGSFAPWFAQSGLQVEYLDAGVDALAGVD
                     VLSPALVVVLGGPIGAYQDALYPFLRDELALIRARLDAGRPLLGICLGAQLIARALGA
                     RVYPGPAVEIGWQGLTLTEAGRASPLAALDGALTSMLHWHGDTFDLPDGAVRLASTPA
                     CANQAFALGAHCLAFQCHPEVDSTRLEAWLIGHASELAHHGIDLAALRRDAGMHGERL
                     RQQALQCLAAWWAQQCAQ"
     gene            3313..>3845
                     /locus_tag="PKN55_10655"
     CDS             3313..>3845
                     /locus_tag="PKN55_10655"
                     /inference="COORDINATES: protein motif:HMM:NF024835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HNX71750.1"
                     /translation="MSTRLKLLRQQHGLSLEQLAQRSGLTKSYVSKVERGLSTPSIAS
                     ALKLARALAVDVAQLFSPHTEAGELCLTRADQRLRIERAGDVGRRVEVLAADMAGKQM
                     QPFVLHPPAEFSDGPAPAGHPGEELVWVLSGEVEMAFPGRHEQLAAGDCVYFRAELPH
                     RIRRLGEAPASVLVVIAR"
     assembly_gap    3846..3945
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3952..4983)
                     /locus_tag="PKN55_10660"
     CDS             complement(3952..4983)
                     /locus_tag="PKN55_10660"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011134444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HNX71751.1"
                     /translation="MRIMIVTDAWRPQVNGVVRTLTETTRELSELGHTVEMITPLDFR
                     TFPCPTYPDIRLSWRPYARVAATLKAFAPDAIHIATEGPLGLAARRWCMANQLRFTTA
                     YHTRFPEYIHARVRLPLSASYAFMRWFHGPSAAVMAPTPSIKSALEGWGFNNVVVWSR
                     GVNTDLFCPGERDRLDDSVPPRFVYIGRVAVEKNIEAFLQLDLPGSKWVVGDGPQMEL
                     LKRRYPNVWFAGVYPQAELARFYRAADVFVFPSLTDTFGLVLLEAMACGTPVAAFPVA
                     GPLDVVADSGAGALNQDLRQACLDAMHIDRCHVRRHAERFSWAAATRQFEAHLCPNPP
                     RMSAQLSAA"
     gene            complement(4971..>5224)
                     /locus_tag="PKN55_10665"
     CDS             complement(4971..>5224)
                     /locus_tag="PKN55_10665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014088471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HNX71752.1"
                     /translation="EARRRGFDGVVCGHIHKAEIRDIDGVLYCNDGDWVESLTALVET
                     PEGALEIVHWPHLLGKPRAASPALDPDDSDSEDTPTCAS"
BASE COUNT          950 a         1710 c         1579 g          785 t
ORIGIN      
        1 gatacgccgc cgccacgccc agctcggaca gggtggcgac ctggtcttcc atcagcgcga
       61 tcagcggcga caccaccacg gtgacgccag gacgcaacaa ggccggcacc tgaaagcaca
      121 gcgacttgcc gccgccggtg ggcatcagca ccagggcatg gtggccggtg gcgacgtgcg
      181 cgacgatttc ggcctgcaaa ccgcgaaact cggggtagcc aaacacctgc tggaggacgg
      241 tcagcgcatc ttgcatggcg ggcaatcaca aacaaaaacg aattgtcgca caaaaaaaac
      301 gccattcgcg cgcggcgaat ggcgtgaagc gttccgttga ctgtggaagc attggggaac
      361 gtagaggaga aaacacaatg tgtttgtgcg cgccgcaaga aaaaagtttc cggccattgc
      421 ggtaaaattt gcgcggcgca ccatccgcca tgcccggcgg cttctgtgcg acacttgtgg
      481 tttttcatca ggagcatccc catgtcgtca cgcgcacttg tcgccgccct gcttgccgtg
      541 ggcctggctg cctgcgccac ccaggaagac cccggccgca gcgcaccgcg cgaccccggc
      601 gccgccaccc aatggcaaaa cctgggcgtc acccccaatg gcaatatcct gcacgagctg
      661 gacaagcaat cgattgtgcg ccatggcgac gtggtgacct tccgcgaccg caagaccatc
      721 ttcaacctga agcgggaaac cttcaccagc ctgccgcgcc acaaggtgtc ggtcaacagc
      781 tggcggattg actgccaggc gcaaacctat caactgctgg ccagcagcct gtttgatgaa
      841 aatggcaagc cggtggccag ctatacgtat aatgacggag aaatccgtcc ggcagccatc
      901 acgcccaatt cggccagtca tcagcaaatg caggcagtct gccgacgccc ctgactctca
      961 gacctgaaag cattcgcatg acggcaatca aacgcgtggt gttcaaccaa aaaggcgggg
     1021 tgggcaaatc caccatcacc gccaacctgg ccgccgtggc cgcccagcaa ggccggcgcg
     1081 ccctggtcgt ggatctcgat ccgcagggta atctgtccca ctatgtatac ggccccgacg
     1141 ccgaccagct gcgcccgtcg gtattcgatt tttttgacca gatcctgaat ttcagcctga
     1201 acagccagcc cacgctggcg catgtgcacg ccacgccgtt tgccggcctg tcgctgatgt
     1261 gctcgcaccc ggagttgggc gagctgggcc ccaagctgga agcgcgctac aagatgtaca
     1321 agctgcgcga cgcgctgaac gaactggcca aggaatttga cgaaatctgg gtggacaccc
     1381 cgccggcgct gaactttttt tcccgctcgg cgctgattgc ctgtgagcgg gtgttgattc
     1441 cgtttgactg tgatgatttt tcccgccggg cgctgtatac cctgctggac aatctgcaag
     1501 aaatccgtgg cgaccataac gccggcctgc aactggaagg catcatcgtc aaccagtttc
     1561 aaccgcgcgc cgcgctgccg gccaagctgg tggccgagct ggccgccgag ggcctgccga
     1621 tgctgaacag caagctgtcg tcgtcggtca aaatccgcga atcgcaccag agcagccagc
     1681 ccatggtgta tctcgacccc aagcacaaac tcagtcagga gtttgtcgcg ctgtatcagg
     1741 aactgagcgg gcgctaannn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnngca
     1861 tcctcacggt ggccgtgcgc cgcctgcgcc agcaagcgct cgcgccagcg cggcggcacg
     1921 ccgggcaagg ccgccacggc aaggcgctcg tcggcggcga cgtttgcctg attcagcgcg
     1981 cgcagcaccc gcgccaattg gctgtctggc catggccgcg ccagcaattg cagcacatcg
     2041 cccgcttgca gccagcccgg ctgcaacacc cgaaaatacc agcccacccg gccgctatcc
     2101 tgcacccgcc gcgccatgtc cggccgggca aagcgcacat tcagcttcca gcagggttgc
     2161 cgcccctggc tgagctgcac ccggcacggc cccagcgcaa acacatcgcc caggcagacg
     2221 tcttgttcca gcaagcccag cccagacagg ttttcgccaa aatcgcccac cgccaggcgc
     2281 aagccggggt cttccgccgc caggctgcgg tagtgctcgg ccgggaaata atgcaaggcc
     2341 ttgtccacgc cgccgtgcac ccggcggtcg gcttgctcat cgccgcgcag gccgagcgca
     2401 tccagccaca atggcccgtg cacggcgtgt ttatcgatgg cgctcagtcg cccattcggg
     2461 ccgagtgggc gcgcctggcc gatgcgcggg gcggcaatcg ccagcggcgg cggccaattc
     2521 actgcgcgca ttgctgcgcc caccacgccg ccaggcattg cagcgcttgc tggcgcaggc
     2581 gttcgccgtg catgcccgcg tcgcggcgca gcgccgccag atcaatgccg tggtgcgcca
     2641 gctcgctggc gtggccgatc agccaggctt ccagccgtgt gctgtccact tccgggtgac
     2701 actgaaacgc caggcaatgc gcgcccaggg caaacgcctg attggcgcaa gccggggtgg
     2761 aggccaagcg cacggcgcca tccggcaggt cgaaggtgtc gccgtgccag tgcagcatgc
     2821 tggtgagcgc gccatccagc gccgccaacg gcgaggcgcg gccggcttcg gtcagcgtca
     2881 gcccttgcca gccgatttcc accgccgggc cggggtagac gcgcgcgccc agtgcgcggg
     2941 caatcagctg cgcgcccagg cagatgccca gcagcggccg gccggcgtcc agccgggcgc
     3001 ggatcagcgc cagttcgtcg cgcaaaaaag gataaagcgc atcctgataa gcgccaatcg
     3061 gcccgcccag caccaccacc agcgcaggcg acagcacatc caccccggcc agcgcatcca
     3121 cgccggcatc cagatactcg acttgcagcc ccgattgggc aaaccacggg gcaaagctgc
     3181 ccaagtcctc aaactgcaca tggcgaatcg ccaatacccg tcctgacatc gcgcgccctc
     3241 gctcccattg aatgacacat cgtgtcttat attacactgt ttgtctcaac aaacaagcgg
     3301 caaggagccg acatgagcac gcgcttgaag ctgctgcgcc agcaacacgg cctgagcctg
     3361 gagcaactgg cccagcgcag cgggctgacc aaaagttatg tgtccaaagt ggagcgcggg
     3421 ctgtccacgc cgtcgatcgc cagcgcgctc aagctggcgc gcgccctggc ggtggatgtg
     3481 gcgcagctgt tcagcccgca caccgaggcg ggcgaactct gcctgacccg cgccgaccag
     3541 cgcctgcgca tcgagcgcgc gggcgacgtt ggccggcggg tggaagtctt ggcggcggac
     3601 atggctggca agcagatgca gccctttgtg ctgcacccgc cagcggaatt cagcgacggg
     3661 ccagcgccgg ccggccatcc gggcgaggaa ttggtgtggg tgttgagcgg cgaggtggaa
     3721 atggcgtttc ccggccgcca cgagcaactg gccgccgggg attgcgtgta ttttcgcgcc
     3781 gagctgccgc accgcatccg ccggctgggt gaggcgccgg ccagtgtgtt ggtggtgatt
     3841 gcccgnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnncgccg ctcaggccgc
     3961 gctcagctgc gccgacatgc gcggcgggtt ggggcacaga tgggcttcaa actgacgggt
     4021 ggccgccgcc cagctgaagc gctcggcgtg gcggcgcaca tggcagcggt cgatgtgcat
     4081 ggcgtccagg caggcctggc gcaaatcctg gttcagcgcg ccagcgccgc tgtcggccac
     4141 cacatccagc ggcccggcca ccggaaacgc cgccaccggc gtgccgcagg ccatggcttc
     4201 cagcagcacc aggccaaagg tgtcggtcag gctggggaac acaaacacat ccgccgcgcg
     4261 gtaaaaacgc gccagttcgg cttgcggata caccccggca aaccacacat tcgggtaacg
     4321 gcgcttgagc agctccattt gcgggccgtc gcccaccacc catttgctgc cgggcaaatc
     4381 cagctgcaaa aacgcctcga tgtttttttc caccgccacc cggccgatgt agacaaagcg
     4441 cggcggcacc gagtcatcca gccggtcgcg ctcgcccggg caaaacaggt cggtgttcac
     4501 cccacggctc cacaccacca cattgttaaa gccccagcct tccagcgcgc tcttgatcga
     4561 cggcgtcggc gccatcaccg ccgcgctggg gccatgaaac cagcgcatga acgcatagct
     4621 ggccgacagc ggcaggcgca cgcgggcgtg gatgtattcc ggaaaacggg tgtgatacgc
     4681 ggtggtgaag cgcaattgat tggccatgca ccagcgccgc gccgccaggc ccagcgggcc
     4741 ttcggtggca atgtggatgg cgtctggggc aaacgccttc agcgtggccg ccacccgcgc
     4801 atacggccgc caggacaggc ggatgtccgg gtaggtgggg cagggaaagg tgcgaaaatc
     4861 cagcggggtg atcatttcca ccgtgtggcc cagctcgctc agctcgcggg tggtttcggt
     4921 cagcgtgcga accacgccat tgacctgcgg ccgccaggcg tcggtgacta tcatgatgcg
     4981 catgtggggg tgtcctcgga gtcggagtca tcaggatcaa gagcaggcga ggccgcgcgc
     5041 ggcttgccca gaagatgtgg ccagtgcacg atttccagcg cgccttccgg ggtttccacc
     5101 agcgcggtga ggctttccac ccaatcaccg tcgttgcagt acagcacgcc gtcgatgtcg
     5161 cggatttcag ccttgtggat atgcccgcac accacgccgt caaacccgcg ccgccgcgcc
     5221 tcgt
//
LOCUS       DAOPYX010000215         5212 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_20070_length_5212_cov_7.680628, whole genome shotgun sequence.
ACCESSION   DAOPYX010000215 DAOPYX010000000
VERSION     DAOPYX010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 5212)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5212)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5212
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20070_length_5212_cov_7.680628"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(66..797)
                     /locus_tag="PKN55_10670"
     CDS             complement(66..797)
                     /locus_tag="PKN55_10670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004285004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HNX71753.1"
                     /translation="MAGHSKWANIKHKKAAADAKRGKIFTRLIKEITVAAKMGGADVG
                     TNPRLRLAVDKAFDANMPKDTIQRAIDRGAGNLEGVNYEECRYEGYGIGGAAVMVDCL
                     TDNKTRTVADVRHAFSKFGGNMGTDGCVSFQFKHCGQLLFAPGTDEDSLMEAALEAGA
                     EDVLSHDDGSFEVITPPYEFSDIRATLEKAGFKADMADVSMKPLNETELTGDDALRMQ
                     KLLDALESLDDVQDVFTSAVMDEEA"
     gene            1125..2996
                     /locus_tag="PKN55_10675"
     CDS             1125..2996
                     /locus_tag="PKN55_10675"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNX71754.1"
                     /translation="MSRRSNRVPLLALLLAGVLSAFLTVQLVDARRQQENLARQEARN
                     LAALLEARLLAGIREIDVALRSVSPRLNPVSLRHNPMPANARLVAEHALEQALPLAGF
                     ADHLGLMNVRGELIYQTGRHPELLEAMSREYFIRMRDDPGLKFFGTRLLVSSDQRSSG
                     IVLARRVEDAQGEFAGVLLATVDQKAILKLFSSIQVGEHGAVALRDDTMATIARVPQH
                     DANSTVLSTRHPLLNALARGEIGGDYTARSVVDQEARMHAWRLLPGTRYVVLVGVAEQ
                     DYLRAWRRMLHAYYAAMSALALLGGAVAWHYRGERRDGRALQASKAQLEASEARFRGM
                     VENLPFPLTITALADRSLLYANARACRLFGMDAELREQGQRPDPSRFYVDPAERASLF
                     QRLARTEQVDNVELQFQRDNGTQFWALVSAVHLVFDHQPAVLCGFHDISSRKLLEDTL
                     RRQAQTDALTGLANRAHTLERAVQLREAALANGTPLAALMLDIDHFKKVNDTRGHACG
                     DAALHGLASTLEHNLRRHDLIGRLGGEEFAVFLPDTALGQAMQLAERLRMQVAITAMA
                     APGEPEPIRITISIGLAMLSGQESLESLLARADDALYQAKREGRNRVVQASAPTHAG"
     gene            complement(3108..4118)
                     /locus_tag="PKN55_10680"
     CDS             complement(3108..4118)
                     /locus_tag="PKN55_10680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX71755.1"
                     /translation="MQLASLSRSLQGQWQRLRQAVVRPSATGDVSSQDAAPAMSRREK
                     AQVSLGEKIAQDRQALYDKLGQMDRNSGLRNSRQSARDKVAMLRERVRMLKAQIAGAH
                     GATLKRLARQLAQVAAQLKQAVSQYAGATSKLSTPSPAAPASAASGAAVSADAQASPT
                     ATVDAASAAPPPAAESVTRIAAATAQTVAEAVLSDATQPATAESRDDASHKEMNAARE
                     REQAKTDEKMDKAFAKEVKDLLRELKQAAELLKRRMRHAQQSAALAELERSLREIEGQ
                     LARLQSGSQSGGEPTTGTPTATAGVDTISVDAVDVGVATSIDVGSGATAGSGAVSAGT
                     GA"
     gene            complement(4178..4645)
                     /gene="dtd"
                     /locus_tag="PKN55_10685"
     CDS             complement(4178..4645)
                     /gene="dtd"
                     /locus_tag="PKN55_10685"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014086339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HNX71756.1"
                     /translation="MRAVVQRVSQASVSVDGVCTGAIGPGLLVLACIESGDGEAELAW
                     MARKLVQLRIFADAHGAMNRSLLDVNGELLAISQFTLAGDCRKGNRPSFVRAERPERA
                     APMFADWLHTLSVQLGKPVPSGVFGADMQVSLVNDGPVTLWLQSPGGEASRTS"
     gene            4845..>5212
                     /locus_tag="PKN55_10690"
     CDS             4845..>5212
                     /locus_tag="PKN55_10690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012697741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HNX71757.1"
                     /translation="MIPIAEFRVPYTRFLDAHGHLASPPPDFQPHDLLPVYRAMALTR
                     AFDAKAVALQRTGQLRTYPSSLGQEAVTVGLASVMRAEDVLLPTYRETGAMLWRGVRM
                     QELLLYWSGDERGSDFAGPR"
BASE COUNT          845 a         1683 c         1656 g         1028 t
ORIGIN      
        1 ccggcccccg gtgctgcaca atacatgcag tcagcggggg ccgggtgtgt gccgggatgg
       61 atgaatcagg cttcttcgtc catcactgcc gaggtgaaca cgtcctgcac gtcgtccagg
      121 ctttccagcg cgtccagcag tttttgcatg cgcagggcgt cgtcgccggt cagctcggtt
      181 tcgttcagcg gtttcatgct gacgtcggcc atatccgcct tgaagccggc tttttccagc
      241 gtggcgcgga tgtcggagaa ttcatacggc ggggtgatga cttcaaacga gccgtcgtcg
      301 tggctgagca cgtcttcggc gccggcttcc agcgcggctt ccatcagcga gtcttcgtcg
      361 gtgcccggcg caaacagcag ctggccgcag tgcttgaact ggaacgacac gcagccgtcg
      421 gtgcccatat tgccgccaaa cttggagaaa gcatgacgca cgtcggccac ggtgcgggtt
      481 ttgttgtcgg tcaggcaatc caccatcacc gccgcgccgc cgatgccgta gccttcgtag
      541 cggcattctt cgtagttgac gccttccaga ttgccggcgc cacggtcgat ggcgcgctgg
      601 atggtgtctt ttggcatatt ggcgtcaaat gccttgtcca ccgccagacg caggcgcggg
      661 ttggtgccca cgtcggcgcc gcccattttg gcggcgacgg tgatttcctt gatcaggcgg
      721 gtaaagatct tgccgcgctt ggcgtcagcg gcggctttct tgtgtttgat attggcccat
      781 ttgctgtgtc cagccatagc aacctcgttg atgtcagtca gtgcttacgc aaaacacccg
      841 cgtgagcggg tgttttgcgt aagctggatg tcaagggcgc gccgtccggc cggcaggctg
      901 gccgggcttg cgcactccaa aaagcgcaat tgaatgaaag agtgtatcac agcacaccag
      961 acggcagcac ggcccgtgcg gtctgctgac tttggccata tcaccggctg gcaccagcgg
     1021 catcatggcg aacaatatgt catactatac gacttgatat cattcgagcg cgcgccgcct
     1081 gccttatgct caagcttccc tcgttgaccg ccctggcgcg cggaatgtcg cgacgcagca
     1141 accgggtgcc cctgctggcc ttgctgctgg ccggggtgct gagtgctttt ctcaccgtgc
     1201 aactggtgga tgcccgccgc caacaagaaa acctggcccg gcaagaagcc cgcaaccttg
     1261 ccgccctgct ggaggcgcgg ctgctggccg gtatccgtga aatcgacgtg gctttgcgca
     1321 gtgtgtcgcc acgactgaat ccggtcagcc tgcgccataa tccgatgcca gccaacgccc
     1381 gcctggtggc ggaacatgca ctggagcagg ctttgccgct ggcaggcttt gccgatcacc
     1441 tgggcttgat gaatgtacgc ggcgagctga tctaccagac gggccgacat cctgagttgc
     1501 tggaggccat gagccgtgag tatttcatcc gcatgcgtga cgatccggga ttgaaattct
     1561 tcggcacccg cctgctggtg tcgtccgacc agcgcagctc gggcattgtg ctggcgcgac
     1621 gggttgagga cgcacagggt gagtttgccg gagtgctgct ggccacggtg gatcagaaag
     1681 ccattctcaa attattttcc agtattcagg tcggcgagca tggcgcggtg gcgctacgcg
     1741 acgacaccat ggccaccatt gcccgtgtgc cgcaacacga cgccaacagc acggtgttgt
     1801 ccactcgcca cccgttactg aacgccctgg cgcgcggcga aatcggtggt gattacaccg
     1861 cacgctcggt ggtggatcaa gaggcgcgca tgcatgcctg gcgcttgctg ccgggcaccc
     1921 gctatgtggt gctggtgggg gtggccgagc aggattatct gcgcgcctgg cggcggatgc
     1981 tgcatgctta ctatgcagcg atgagtgcgc tggcgctgct gggcggggcg gtggcctggc
     2041 attatcgggg cgagcgccgc gatggccgcg cattacaggc cagcaaagcc cagttggaag
     2101 ccagcgaagc gcggtttcgc ggcatggtgg aaaatctccc gtttccgctg accatcaccg
     2161 cgctggccga ccgcagcctg ctgtatgcca atgcccgtgc ctgccggctg tttggcatgg
     2221 atgccgagct acgcgagcag ggccagcggc cagatcccag ccgtttttat gttgatcctg
     2281 ccgagcgtgc cagtctgttc cagcgcctgg cccgtaccga gcaagtagat aatgtggaat
     2341 tgcaatttca gcgcgacaat ggcactcagt tctgggcgct ggtgtcggcg gtgcatctgg
     2401 tgtttgatca ccagccggcg gtgctgtgcg ggtttcatga catcagcagc cgtaagctgc
     2461 tggaagatac tctgcgccgt caggcacaaa ctgacgcact gaccgggctg gccaaccgcg
     2521 cccataccct ggagcgcgcc gtgcaattgc gcgaggctgc gctggccaat ggcacgccac
     2581 tggcggcgct gatgctggac atcgatcact tcaagaaggt caacgacacc cgtggtcacg
     2641 cctgtggcga tgctgcgctg catgggctgg ccagcaccct ggagcacaat ctgcgccgcc
     2701 acgacctgat cggccgcttg gggggcgaag agtttgccgt gtttctgccg gacacggcac
     2761 tgggccaggc catgcaattg gccgagcgcc tgcgcatgca agtggccatc accgccatgg
     2821 ccgccccggg tgagcctgag ccgatccgca ttaccatcag cattggcctg gccatgctga
     2881 gcgggcagga atcgctggaa tccctgctgg cgcgtgccga cgatgcgctg tatcaggcca
     2941 agcgtgaggg ccgcaaccgg gtggtgcaag ccagcgcgcc aactcacgct ggctaagagc
     3001 ggctaacaaa accctcctgg cgttgttgcc cgaccttgcc gtactccccg tactgtctgc
     3061 ggtcacacgc ttggccagga ccgcttcgct gggtgttgtg agcgacgcta agcgccagtg
     3121 ccggctgata cggcgccact gcctgccgtc gccccgctac ctacatcaat tgatgtcgcc
     3181 acgccgacat ccactgcatc cacactgatg gtgtccactc ccgctgtcgc ggtcggcgtg
     3241 ccggtggtgg gctcgccgcc agactgagac ccgctttgca atcgggccag ttgcccttcg
     3301 atttcgcgca agctgcgctc cagctcggcc agcgctgcgc tttgctgcgc atggcgcatc
     3361 cggcgtttca gcagctcggc ggcttgcttg agctcgcgca gcaggtcttt cacctctttg
     3421 gcgaacgcct tgtccatttt ttcatcggtc ttggcttgct cgcgctcgcg cgcggcattc
     3481 atttccttgt gcgaggcatc gtcacgggat tcggctgtgg cgggctgggt ggcatcgctc
     3541 agcacggcct cggcgaccgt ctgtgcggta gcggcggcaa tgcgggtgac ggattctgcg
     3601 gcagggggcg gcgcagccga ggccgcgtcc accgtggctg tcgggctggc ctgggcatcc
     3661 gctgacacgg cggcgcctga ggcagcgctc gctggcgcag cggggctggg cgtgctcagc
     3721 ttgctggtgg cgccagcgta ctgcgacacg gcttgtttga gctgcgccgc cacctgcgcc
     3781 aactgacggg ccaggcgctt gagtgtggcg ccgtgcgccc cggcaatttg cgccttgagc
     3841 atgcgcaccc gctcacgcag catggccacc ttgtcgcgtg cgctttgccg actgttgcgt
     3901 aagccgctgt tgcgatccat ctggccgagt ttgtcgtaga gcgcttgtcg gtcctgggct
     3961 attttttcgc ccaggctgac ttgggctttt tcgcgccgcg acatcgcggg tgcggcatcc
     4021 tggctgctga cgtcgccggt ggcgctgggg cgcaccaccg cctggcgcag gcgctgccac
     4081 tggccttgca ggctgcggct aagagaggca agttgcatgc gggtggctcc agtgtgatcg
     4141 ggatatgaca atatcggcca gcccgccgca aactcaatca gctggtacgg ctcgcctctc
     4201 cccctgggct ttgcagccat agcgtcaccg gcccatcgtt caccaggctc acctgcatat
     4261 cggcgccaaa caccccgctg ggcacgggct tgcccaactg gaccgacagg gtgtgcaacc
     4321 agtcggcaaa catcggcgcg gcgcgctcgg ggcgctcggc gcgcacaaac gacggccggt
     4381 tgcccttgcg gcaatcgccg gccagagtga actggctgat ggccagcagc tcgccgttca
     4441 cgtccagcag cgagcggttc atggcgccgt gtgcatcggc aaaaatgcgc agctgcacca
     4501 gcttgcgcgc catccatgcc agctcggcct cgccatcgcc gctttcaata caagccagca
     4561 ccagcaaacc agggccaatg gccccggtac acacgccatc gacactgacc gaagcctgac
     4621 tcaccctttg caccactgcg cgcatgcgct cacctttgat ttttgcatga ttcaccgcgc
     4681 caactggcgc ccagcctgac attatcgcat gccgatttcg ctgcattgca gctaccgcag
     4741 tgcagaatga cgttccagca gaactcggct aaggtggaaa tgccctcaca aggggcatcg
     4801 actgatcgcc cacggtcagc gctttcgaca aaggagacac caccatgatc ccgattgccg
     4861 aattccgcgt cccctacacc cgttttctgg acgcgcacgg ccaccttgcc tcacccccgc
     4921 ccgatttcca gccgcacgat ttgctgccgg tgtaccgcgc catggcgctg acccgcgcct
     4981 ttgacgccaa ggcggttgcc ttgcagcgca ccggccagct gcgcacctat ccgtcctcgc
     5041 tgggccagga ggccgtcacc gttggcctgg ccagcgtgat gcgcgccgag gacgtgctgc
     5101 tgcccaccta ccgcgaaacc ggggccatgc tctggcgcgg cgtgcgcatg caagagctgc
     5161 tgctgtactg gagcggcgac gagcgcggca gcgactttgc cggcccgcgc ga
//
LOCUS       DAOPYX010000216         5122 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_20566_length_5122_cov_9.581212, whole genome shotgun sequence.
ACCESSION   DAOPYX010000216 DAOPYX010000000
VERSION     DAOPYX010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 5122)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5122)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5122
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20566_length_5122_cov_9.581212"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..494)
                     /locus_tag="PKN55_10695"
     CDS             complement(<1..494)
                     /locus_tag="PKN55_10695"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNX71758.1"
                     /translation="MSPQNGPVLRLSGVHKHYQLGAHRIDALRGVDLDVQAGELLALT
                     GPSGSGKSTLLNLCGLIDTPDAGDIELNGQPVNALDETARTLLRRDALGFVFQNFNLI
                     PVMTVAENVDYPLFLADVPPAERRARVAAQLAAVGLAEHAHHRPDALSGGQRQRVAIA
                     RALI"
     gene            complement(506..994)
                     /locus_tag="PKN55_10700"
     CDS             complement(506..994)
                     /locus_tag="PKN55_10700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX71759.1"
                     /translation="MPAHAVTEAQFMPALHVFLQASDGDDAAIEPAAEAFAALSQAEP
                     GNAVLLAYRGAATAMLAKTMWMPWKKMRQAEDGLAMLDKSLAMLATGQPGLSPSGAAT
                     TLEVQYVAARTFLAVPTVMHRHEQGMRLLTQVLTHPQLAAAPAALRERVRQTAKQYGV
                     GL"
     gene            1232..2119
                     /locus_tag="PKN55_10705"
     CDS             1232..2119
                     /locus_tag="PKN55_10705"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HNX71760.1"
                     /translation="MRFDFVDLRLLVHVAEAGNLTAGAARLPIALSAASQRIRKLETL
                     FGVNLFERERDGVRLTADGERCVEHARLLLRGADRMQRELTHPAQQGRHVLRLAATTV
                     TTAEFLPDVLGRFLALHPQVDVEIDERQSAAAIQAIRDGEHDLGIIDGNTGAERLALL
                     PFSRDKLVLVAHAGHPLAARQQVSFAEALDHPFVALRPDSGMQQFIERAALLHGGKLQ
                     VRLRVPSFEALYRLVAAGAGLGVLPEAAALRYRSAGQPVTIVEFRDRWAERELKLCAR
                     DFVSLSPLQQTFVAFLTPA"
     gene            complement(2140..3453)
                     /gene="tilS"
                     /locus_tag="PKN55_10710"
     CDS             complement(2140..3453)
                     /gene="tilS"
                     /locus_tag="PKN55_10710"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HNX71761.1"
                     /translation="MDYLESALHEAFPEARHIEVALSGGVDSVCLTYLLADLAKRRGF
                     RLSAVHVNHQINLRAGDWQEFCQWLCASLNIPLRTAKVEVNWGGGDSLEAEARQARYA
                     IFAQSQADVLALAHHADDQAETILLQLLRGAGPHGLAAMPVTRALTEHTQLWRPLLNA
                     TRDQLEALATVEDWIWIEDDSNDDTRLRRNFLRHDILPRLAQGYPDYREQLLRSVRRA
                     ADAAALLDELAASDLELMVGADSSLDLDMLAHVSLPRARNALFAWVEERGGVLTPDGL
                     DEALVQLLDAAPDANPMIDVGAWRLTRYRQRAYLIRPFTTPSAPIAFEFSPEQLTLDF
                     PDWGGRLRFNPIANGGLRQVALTGGLTLRPRQGGESLPQNGVNKTLKNLLQEAGMPPY
                     RRERLPLIYQNDTLLAAPGAGINTELLAAEPDVGYVPVWLPYDGY"
     gene            complement(3458..4426)
                     /locus_tag="PKN55_10715"
     CDS             complement(3458..4426)
                     /locus_tag="PKN55_10715"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012696340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HNX71762.1"
                     /translation="MKTTFLDFEQPIAELESKIEELRFVQDDSVLDISEEISRLQKKS
                     QELTKAVYAKLTPWQISQVARHPQRPYTLDYIKSIFTDFHELHGDRAYADDPAIVGGL
                     ARFNGQSVMVIGHQKGRDTKEKIYRNFGMPRPEGYRKALRLMKTAEKFNLPIITFIDT
                     PGAYPGIGAEERGQSEAIGRNLYEMTRLRVPIICTVIGEGGSGGALAIAVGDQLNMLQ
                     YSTYSVISPEGCASILWKSAEKAPEAAETLGITASRLKTLGLIDKVINEPLGGAQRDH
                     AAMMQSMKRALAEALRDLQDAPVDELLKQRFDRLMNYGRFKDDAEA"
     gene            complement(4445..4705)
                     /locus_tag="PKN55_10720"
     CDS             complement(4445..4705)
                     /locus_tag="PKN55_10720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX71763.1"
                     /translation="MLFDKSAIGRACCACVRTCLRAIIAVFAAFACQPAAMVLCVNTQ
                     TNHHLPWIKAVPGMQALLRHFATIRTTVSWQTPKASCMIARF"
     gene            4861..4937
                     /locus_tag="PKN55_10725"
     tRNA            4861..4937
                     /locus_tag="PKN55_10725"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4895..4897,aa:Val,seq:cac)
     gene            4942..5018
                     /locus_tag="PKN55_10730"
     tRNA            4942..5018
                     /locus_tag="PKN55_10730"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4976..4978,aa:Asp,seq:gtc)
BASE COUNT          899 a         1570 c         1678 g          975 t
ORIGIN      
        1 ttgatcagcg cgcgggcaat cgccacccgc tggcgctggc cgccgctgag cgcatccggg
       61 cggtggtggg cgtgttcggc caggcccacg gccgccagtt gcgccgccac ccgcgcgcgg
      121 cgctcggccg gcggcacatc ggccagaaac agcgggtaat cgacgttctc ggccacggtc
      181 atcaccggaa tcagattgaa gttctgaaac acaaagccca gcgcatcgcg gcgcagcagg
      241 gtgcgcgcgg tttcatccag cgcattgact ggctggccgt tgagctcgat gtcgcccgcg
      301 tccggggtgt cgattaaccc gcacaggttc aatagcgtgc ttttgccgct gccggacggg
      361 ccggtcagcg ccaacagctc gccggcctgc acgtccaggt ccacgccgcg caaggcgtcg
      421 atgcggtgcg cgcccagttg gtagtgttta tgcacgccag acaggcgcaa tactggccca
      481 ttctgtggcg acatcaagac cgcgctcata agcccacccc atattgtttg gcggtttgcc
      541 gcacgcgctc gcgcaaggcg gcaggcgcgg cggccagttg cggatgggtc aacacctgcg
      601 tcagcagccg catgccctgt tcgtggcgat gcatcaccgt gggcaccgcc agaaaggtgc
      661 gcgccgccac atactgaact tccagcgtgg tggccgcgcc gctcgggctg agccctggct
      721 ggcccgttgc caacatggcc agtgatttat ccagcatggc caggccatcc tcggcctgac
      781 gcattttttt ccagggcatc cacatggttt tggccagcat ggccgtggcc gcgccacggt
      841 aggccagcaa taccgcattg ccaggttcgg cttgggacaa tgcagcaaat gcctcggcgg
      901 ccggctcgat ggcggcatcg tcgccatcgc tagcctggag aaatacatgc aaagccggca
      961 tgaattgcgc ttcggtgacg gcatgtgccg gcatggcgct cagccatagc gcacaacagg
     1021 cacaaaacca tagggatttg aacatcagga gcctcgctat caggggggtc atgctgacag
     1081 tatggcgagg tggctgcgcg ggctcagtaa gcatgtgacg aaaggggtta ttcacggcgt
     1141 cagccgggat gccaggcccg ccaaacgcgg gctcgccatg tcggcaaatt tccccgatga
     1201 tggcgaaccc atggctgtct ggatctctgc aatgcgattt gattttgtcg atctgcgcct
     1261 gttggtgcat gtggccgaag ccggcaacct cactgccggc gccgcgcgcc tgccgattgc
     1321 cctgtcggcg gccagccagc gcatccgcaa gctggaaacc ctgtttggcg tgaatttgtt
     1381 cgagcgcgag cgcgacggcg tgcggctgac cgccgatggc gagcgctgcg tcgagcatgc
     1441 ccggctgttg ctgcgcgggg ccgaccgcat gcagcgcgag ctgacccacc cggcgcagca
     1501 aggccgccat gtgttgcgcc tggccgccac cacggtgacc acggcggagt ttttgcccga
     1561 tgtgctcggg cgctttttgg cgctgcaccc gcaggtggac gtggaaatcg acgaacggca
     1621 aagcgcggcg gcgattcagg ccatccgcga tggcgagcac gatctgggca ttatcgacgg
     1681 caacaccggc gccgagcggc tggcgctgct gccgttcagc cgcgacaagc tggtgctggt
     1741 ggcgcacgcc ggccatccgc tggcggcgcg ccagcaggtg tcgtttgccg aagcgctgga
     1801 tcatccgttt gtcgccttgc gcccggacag cggcatgcag caatttatcg agcgcgccgc
     1861 gctgctgcac ggcggcaagc tgcaagtgcg gctgcgcgtg cccagtttcg aggcgctata
     1921 ccgattggtg gcggcggggg cgggtttggg ggtgttgcca gaagcggccg cgctgcgcta
     1981 tcgcagcgcc ggccagccgg tgaccattgt cgagtttcgt gatcgctggg ccgagcgcga
     2041 gttaaagctg tgtgcgcgcg actttgtcag cctgtcgccc ttgcagcaaa ccttcgtggc
     2101 gtttttgacg ccggcctgac gccgtgctgc cgggcccgct caatagccgt cgtaaggcag
     2161 ccacaccggc acatagccga cgtccggttc agcggccagc agctcggtgt tgatgccagc
     2221 gccgggcgcg gcgagcaggg tgtcgttctg atagatcagc ggcaggcgct cgcgccggta
     2281 aggcggcatg ccggcttctt gcagcaggtt tttcagggtt ttgttcacgc cgttttgcgg
     2341 caggctctcg ccgccctggc gcgggcgcag ggtcaggccg ccggtcagcg ccacctggcg
     2401 caggccgcca ttggcaatcg gattaaagcg cagccggccg ccccagtcgg ggaagtccag
     2461 ggtgagctgc tcgggcgaga attcaaaggc aatcggcgcg gagggagtgg taaacggacg
     2521 aatcagatag gcgcgctggc gatagcgggt aagccgccag gcgccaacgt cgatcatcgg
     2581 gttggcgtcg ggcgctgcgt ccagaagctg caccagcgct tcgtccagcc catccggggt
     2641 gagcacgccg ccgcgctctt ccacccaggc aaacagcgca ttgcgtgcgc gcggcagcga
     2701 cacatgggcg agcatgtcca ggtccagcga gctgtcggcg ccgaccatca gttccaggtc
     2761 ggacgccgcc agttcgtcca gcagcgcggc ggcatcggcg gcacggcgca cgctgcgcaa
     2821 tagctgctcg cggtagtccg ggtagccttg cgccaggcgc ggcaggatgt cgtggcgcaa
     2881 aaaattgcgc cgcaagcggg tgtcgtcgtt gctgtcgtct tcgatccaga tccagtcttc
     2941 caccgtggcc agcgcttcca gttggtcgcg ggtggcgttc agcagcggcc gccataactg
     3001 ggtgtgctcg gtcagcgcgc gcgtcaccgg catggcggcc aggccgtgcg ggccggcgcc
     3061 gcgcagcagt tgcagcagga tggtttccgc ctgatcgtcg gcgtggtgcg ccagcgccag
     3121 tacatcggcc tggctctggg caaaaatggc gtagcgcgcc tgacgggctt cggcctccag
     3181 gctgtcgccg ccgccccagt tgacctccac cttggcggtg cgcaagggaa tgttcagcga
     3241 ggcgcacaac cattggcaga attcctgcca gtcgccggcg cgcagattga tttgatgatt
     3301 gacatgcacg gcagacaggc gaaagccgcg ccgtttggcc aggtcggcca agagataggt
     3361 gaggcagacg gaatccaccc caccgctgag cgccacttcg atatggcgcg cctctggaaa
     3421 cgcctcgtgc aaggcgcttt ccagatagtc cacccaatca agcctcagcg tcgtccttga
     3481 atcggccata gttcatcaag cggtcaaagc gttgtttgag cagttcatcc accggggcgt
     3541 cctgcagatc gcgcaacgct tcggccagcg cgcgcttcat ggattgcatc atcgccgcat
     3601 ggtcgcgctg ggcgccgccc aagggctcgt tgatgacttt gtcgatcagg cccagggttt
     3661 tcagccggct ggcggtgatg cccagggttt cggcggcctc gggggctttt tcggcgctct
     3721 tccacagaat ggaggcgcag ccttccggcg aaatcaccga atacgtggag tattgcagca
     3781 tgttcagctg gtcgcccacg gcaattgcca gcgcgccgcc agagccgccc tcgccgatca
     3841 ccgtgcagat gatgggcacg cgcaggcggg tcatttcata cagattgcgg ccaatggctt
     3901 cggactgacc gcgttcttct gcgccgatgc cagggtaggc gccgggggtg tcaataaagg
     3961 tgatgatggg cagattgaat ttttctgcgg ttttcatcag ccgcaaggcc ttgcgatagc
     4021 cttccgggcg cggcatgcca aagttacggt agatcttttc tttggtgtcg cggcccttct
     4081 ggtggccgat caccatcacc gactggccat tgaagcgggc caggccgccc acaatggccg
     4141 gatcgtcggc ataggcgcgg tcgccgtgca gctcgtgaaa gtcggtaaag atgcttttga
     4201 tgtaatccag cgtatacggg cgctgcgggt ggcgcgccac ctgggagatc tgccacgggg
     4261 tgagcttggc gtacaccgcc ttggtcagtt cctggctttt cttttgcaga cggctgatct
     4321 cttccgagat gtccagcacc gaatcatcct ggacaaagcg cagctcttcg atcttgcttt
     4381 ccagttcggc aatgggctgc tcgaaatcga gaaaagtggt tttcatgcgt gacatcggtc
     4441 gatttcagaa tcgggcaatc atacaggagg cctttggcgt ttgccatgaa acggttgttc
     4501 ggattgtggc gaaatggcgc aaaagcgcct gcataccggg cactgccttg atccagggca
     4561 gatggtgatt ggtttgtgta tttacgcata agaccatcgc ggcaggctgg catgcgaatg
     4621 cggcaaacac ggcgattatc gcccgcaggc aggtgcgcac gcatgcacag caggcacgcc
     4681 caattgccga tttatcaaac aacatgctgt tttcaaaaaa tattcaaggt agttcacccg
     4741 ccatccgaca tggtccggca agaaaaaatg cacatttttg caaaaaaacg cttgcacacc
     4801 tcaggggcct atggttaaat gcgcacctct tcgctgcaac gcaaacagca acgcagcgat
     4861 gggcggttag tctcagttgg tagagcactg ccttcacacg gcaggggtca ctggttcgag
     4921 tccagtaccg cccaccattt aggagtggta gttcagttgg ttagaatacc ggcctgtcac
     4981 gccgggggtc gcgggttcga gtcccgtcca ctccgccaac cccaagcagt actcagcaat
     5041 accgtgcatt cgcgcaatct ctgcccgctg gagtggtagt tcagttggtt agaataccgg
     5101 cctgtcacgc cgggggtcgc gg
//
LOCUS       DAOPYX010000217         5114 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_20606_length_5114_cov_11.814390, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000217 DAOPYX010000000
VERSION     DAOPYX010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 5114)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5114)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5114
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20606_length_5114_cov_11.814390"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(95..169)
                     /locus_tag="PKN55_10735"
     tRNA            complement(95..169)
                     /locus_tag="PKN55_10735"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(135..137),aa:Thr,seq:ggt)
     gene            complement(184..257)
                     /locus_tag="PKN55_10740"
     tRNA            complement(184..257)
                     /locus_tag="PKN55_10740"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(223..225),aa:Gly,seq:tcc)
     gene            complement(291..375)
                     /locus_tag="PKN55_10745"
     tRNA            complement(291..375)
                     /locus_tag="PKN55_10745"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(339..341),aa:Tyr,seq:gta)
     gene            complement(540..794)
                     /locus_tag="PKN55_10750"
     CDS             complement(540..794)
                     /locus_tag="PKN55_10750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhL family 4Fe-4S dicluster ferredoxin"
                     /protein_id="HNX71764.1"
                     /translation="MSLMITDECINCDVCEPECPNHAISQGEEIYQIDPDLCTQCVGH
                     YDEPQCQQVCPVDCIPLDPNHEESEEQLMKKYLIISGKAA"
     gene            complement(861..1421)
                     /gene="rsmD"
                     /locus_tag="PKN55_10755"
     CDS             complement(861..1421)
                     /gene="rsmD"
                     /locus_tag="PKN55_10755"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018746378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HNX71765.1"
                     /translation="MAKAGKNTVRIIGGEWRRRLLSFPDLPGLRPTPDRVRETVFNWL
                     GQTLDGLDVLDAFAGSGAMGLEAASRGARRVMLVEKAAPAAQSLRNQARELGMSRVEV
                     TCADALAWLMHCRDSFDVIFLDPPFASELLARILPLAAERVRADGCIYIECGQWPTIP
                     DGWQLVRSGQAGAVHYGLLRRVTDES"
     gene            complement(1551..2873)
                     /locus_tag="PKN55_10760"
     CDS             complement(1551..2873)
                     /locus_tag="PKN55_10760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014085755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HNX71766.1"
                     /translation="MFANWRAGAALLLISLGWLTPAQAVDIQRWQTAHGAKVLFVASH
                     DNPLLDVRVDFDAGNRRDSADKPGVSDLTLTLLDAGTDALSEEAIKARLADLAAQLSG
                     SSDIERAGVGLRTLSDPAQREPALALLADMLARPSFPAALLAREKAQMLTALREAEDD
                     PAGRGERALRRLMYGAHPYALSARINADSVRRISRDDVLRFWRAHYTARRAVVSLVGD
                     ISRADAERIAEQLTARLPAGADALKPIPPVSAPAQGGREQLLHSGTQTHLFLGMPVLR
                     RDDPDYFPLLVGNYVLGGGGFDSRLMKEVRDKRGLTYGVSSHFSPMEQAGEFEIALST
                     RNAQADTALKVVEDTIAQFIAEGPSEDELAQAKRHIIGGFPLRLDSNLKLMGYVSMLG
                     QYDLPNEWLNRYPDKVAAVTLAQVRDAWKRRLSLAALSQVRVGPKPAP"
     gene            complement(2863..4245)
                     /locus_tag="PKN55_10765"
     CDS             complement(2863..4245)
                     /locus_tag="PKN55_10765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003747659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HNX71767.1"
                     /translation="MSQSLRLGAVLSLSAALCAAPVWADAPQEATLANGLKVVVKVDR
                     RAPVAVSQLWYRVGSVDEVSGITGVSHALEHMMFKGTPKVPAGEFSRRIAALGGKENA
                     FTSRDYTVYFQQLANHTLGEAMALEADRMAHLNIAEADFASEISVIREERRQRTEDQP
                     TGVLFEQLYAAAITANPARQPVIGWMADLERMRADDLRQWRERWYAPNNATLVVVGDV
                     DPKAVFQLAEQHYGRIPARTLPARAVLPEPSPYGERRLTLKLPAQQSYVALAWPAPRL
                     SQLNEVDPYALDMLAAVLDGHAASRLPRTLVREQRLASSVGASYDMSGRGASLFTVIA
                     TPAEGKTTAQLEAALRSQVGKLAREGVSEAELARVRTQLKAARVYEKDSMFGQAMKIG
                     QLEALGFSWRDDAEIDRRLDAVTPAQVQAAARRYLTDDRLTVAVLAAQATPPGQAPAA
                     PLTEGGAHVR"
     gene            4361..>5114
                     /locus_tag="PKN55_10770"
     CDS             4361..>5114
                     /locus_tag="PKN55_10770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004285177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signaling recognition particle receptor family
                     protein"
                     /protein_id="HNX71768.1"
                     /translation="MFSFFKKKPKPEDLAAPQAAATPIAEATAPVNPSTAEPLAPAAP
                     AVIAAPAPAVTPVEPEAPRKQSWTERLKAGLSKTRDKLGKSLAGLFGGGKIDEDLYEE
                     LETVLLTADMGVPATMHLLKDVRERVTLRGLKDASELKQALKDSLSELIAPLQIPLDT
                     SGHQPCVIMLAGVNGAGKTTSIGKLAKYFQSQGKSVLLAAGDTFRAAAREQLMAWGER
                     NNVTVIAQQGGDSAAVCYDAIQAAVARKIDIVL"
BASE COUNT          980 a         1704 c         1531 g          899 t
ORIGIN      
        1 cgatggtgcc aacgttgacg tgcggtttgg tccgttcgaa cttttcctta gccatgatga
       61 aaatgcccta aaaagctgat agtcgaaaaa attttggtgc ccatgggcag aattgaactg
      121 ccgacctctc ccttaccaag ggagtgctct acccctgagc tacatgggcc aatagactta
      181 ctttggagcg ggtgaaggga atcgaaccct cgtcttaagc ttggaaggct tcagctctac
      241 cattgagcta cacccgcaat tccaactaaa aacgcacgcc gctaaaaaac tggtggaggg
      301 agaaggattc gaaccttcga aggcagagcc gtcagattta cagtctgatc cctttgaccg
      361 ctcgggaatc cctccgttca agagatgcgc attatgatct cacacctcga tctcgtcaac
      421 acttttctgg caaaaaattt ggatattttt tcaaaaaata cattagccat tgtttttaca
      481 acaaaaaacc cgtcaacttt cgtcaacggg ttttgaccaa accggccacg gcgcaaacat
      541 caagccgctt tgccagagat gatcaggtat ttcttcatca gttgttcttc gctttcctcg
      601 tggttcggat caagcggaat gcagtcaacc gggcacacct gctggcattg cggctcatca
      661 tagtggccca cgcactgggt acagaggtca ggatcgatct gatagatttc ctcaccctgc
      721 gagatggcgt ggttggggca ttccggctca cagacatcgc aattgatgca ttcgtcggtg
      781 atcatcaaag acatagtatt ttcctcaagt attcactgtt tgaattgtgg cacttccctt
      841 cctcactcgc aaggaagggt tcaggattcg tccgtcacgc gtcgcagcag gccgtaatgc
      901 accgccccgg cctgtccgct gcgcaccaac tgccagccat ccgggatggt cggccactga
      961 ccgcactcta tatagatgca gccatcggcg cgcacccgct cggccgccag cggcaagatg
     1021 cgcgccagca gttcggaagc aaacggcgga tccagaaaaa tcacgtcaaa actgtcacgg
     1081 cagtgcatca gccaggccag ggcgtcggcg caggtgactt ccacccgcga catgcccagc
     1141 tcacgcgcct gattgcgcag cgattgggcg gccggcgcgg ccttctccac cagcatcacc
     1201 cgccgggcgc cgcgcgaagc ggcctccagg cccatggcgc cgctgccggc aaaggcgtcc
     1261 agcacgtcca gaccatccag cgtctggccc agccagttga acacggtttc gcgcacgcgg
     1321 tctggcgtcg ggcgcaggcc aggcaggtcc ggaaagctca gcagtcgccg ccgccactcg
     1381 cccccaatga tccgcactgt attcttgccc gccttcgcca tgcccactcc ttgtaacttc
     1441 accctgaaac gcaacagggg gcaacattat gcgccccccg tgccgaagaa gaaaccttct
     1501 tgctcaaaca gctgtatgga tgccagccga ctgacggcgg gctgattcgg tcatggcgcc
     1561 ggcttgggcc ccacccgcac ctgactgagc gccgccaggc tcaagcggcg cttccaggcg
     1621 tcgcgcactt gcgccagcgt caccgccgcc accttgtccg ggtagcggtt cagccactca
     1681 ttgggcagat cgtactgacc cagcatcgac acatagccca tcagcttcag attgctgtcc
     1741 agccgcagcg gaaaaccgcc aatgatatgg cgcttggcct gggccagttc gtcttcgctc
     1801 gggccctcgg caataaactg ggcgatggtg tcttccacca ctttcagcgc agtgtccgcc
     1861 tgggcgttgc gcgtgctcag ggcaatttca aactcgccgg cctgctccat cgggctgaaa
     1921 tggctggaaa caccataggt cagcccacgc ttgtcgcgca cctctttcat cagccgcgaa
     1981 tcaaaaccgc cgccgcccag cacatagttg ccgaccaaca agggaaaata atccgggtcg
     2041 tcgcgccgca gcaccggcat gcccaggaac aaatgggttt gtgtgccgct gtgcaacagc
     2101 tgctcgcgcc cgccctgcgc tggcgcagac accggcggaa tcggcttgag cgcgtccgcg
     2161 ccggccggca ggcgggcggt gagctgctcg gcaatgcgtt cggcgtcggc gcggctgatg
     2221 tcgcccacca gcgacaccac cgcgcgccgg gcggtgtaat gcgcgcgcca gaagcgcagc
     2281 acatcgtcgc ggctgatgcg gcgcacgctg tcagcgttga tgcgcgccga caaggcgtag
     2341 ggatgcgcgc catacatcag gcggcgcaag gcgcgctcgc cgcgcccggc cggatcgtct
     2401 tcagcctcgc gcaacgcagt cagcatctgg gctttttctc gcgccagcag cgccgccgga
     2461 aagctgggcc gcgccagcat atcggccaac aaggccagcg ccggctcgcg ctgcgccggg
     2521 tcggacaagg tgcgcaagcc aaccccggcg cgctcgatat cgctggagcc gctcagttgc
     2581 gcggccaagt cggccagacg cgccttgatg gcctcttcgc tcagcgcatc ggtgccggcg
     2641 tcaagcaagg tcagcgtcag gtcagagaca cccggcttgt cggcgctgtc gcggcggttg
     2701 ccggcgtcaa agtccacccg cacatccagc aagggattgt cgtggctggc gacaaacagc
     2761 accttggcgc cgtgcgccgt ttgccagcgt tgaatgtcca ccgcctgcgc cggcgtcagc
     2821 caacccagac tgatcaatag cagggcagcc cctgcgcgcc agttagcgaa catgtgcgcc
     2881 tccttcagtc aagggtgccg ctggcgcctg gccgggcggc gtggcctgag cggccagcac
     2941 ggccacggtc agacggtcgt cagtcagata acggcgggcg gcggcctgca cctgcgccgg
     3001 cgtcaccgcg tccagccgac ggtcgatttc ggcgtcatcg cgccaggaaa accccaatgc
     3061 ttctaactgg ccaattttca tggcctggcc aaacatcgag tctttttcat agacccgcgc
     3121 ggctttcagc tgggtgcgca cccgcgccag ctcggcttcg ctgacacctt cgcgcgccag
     3181 cttgcccacc tggctgcgca gggcggcttc cagctgggcg gtggtcttgc cctcagccgg
     3241 cgtggcgatc acggtaaaca ggctggcgcc gcgcccgctc atgtcgtagc tggcgcccac
     3301 gctgctggcc aggcgctgtt cacgcaccag ggtgcgcggc aaacgcgagg cggcatggcc
     3361 atccagcacg gcggccagca tatccaatgc gtaagggtct acttcattca gttggctcag
     3421 gcgcggcgcc ggccaggcca gcgccacata gctttgctgg gcgggcagtt tcagcgtcag
     3481 ccggcgctcg ccgtatgggc tgggctcggg caacaccgcg cgcgccggca gggtgcgcgc
     3541 cgggatgcgg ccatagtgct gctcggccag ctgaaacacc gccttgggat cgacatcgcc
     3601 caccaccacc agggtggcgt tgttcggcgc gtaccagcgc tcacgccact ggcgcagatc
     3661 gtcggcgcgc atgcgctcca ggtcggccat ccagccgatc actggctggc gcgccggatt
     3721 ggcagtgatg gccgccgcgt acagctgctc aaacagcacg ccagtgggct ggtcttcggt
     3781 gcgctggcgg cgctcttcac gaatcacgct gatctcgctg gcaaaatccg cctcggcgat
     3841 gttcaaatgc gccatgcggt ctgcttccag cgccatcgcc tcgcccaggg tgtggttggc
     3901 cagttgctgg aaatacaccg tgtagtcgcg gctggtgaag gcgttttcct tgccgcccag
     3961 cgcggcaatg cggcgggaga actcacccgc aggcactttc ggcgtccctt tgaacatcat
     4021 gtgctccagc gcgtgcgaca cgccggtgat gccggacact tcgtccacac tgcccacccg
     4081 ataccacagt tgcgacacgg ccaccggcgc gcggcggtct accttgacca ccactttcag
     4141 gccattggcc agcgtggctt cctgtggcgc atcggcccag accggcgcgg cgcacaaggc
     4201 ggcggacagg ctcaacaccg cgcccaaacg gagggattga gacatggttt tcccgttctc
     4261 catgaaaact tattacaatc gcaggcaaga tgccgtcaga cttgcgtctg cacaggtgcg
     4321 catcatgaac caaacgccgg gaagttgaca ccctgacacc atgtttagtt tcttcaagaa
     4381 aaaacccaaa cccgaagacc tcgctgcgcc gcaagccgcg gccacgccta ttgccgaagc
     4441 cactgcgccg gtcaacccaa gcacggccga gccgctcgcc ccagccgcgc cggcggtgat
     4501 cgctgcgcca gcgccggccg taacgccggt cgagccagaa gcgccgcgca agcagagctg
     4561 gaccgaacgg ctgaaagccg gcctgtcgaa aacccgcgac aagctgggca aatcgctggc
     4621 cgggctgttt ggcggcggca agatcgacga agacctgtac gaagagctgg aaaccgtgct
     4681 gctgaccgcc gatatgggcg tgccggccac catgcacctg ctcaaggacg tgcgcgagcg
     4741 ggtcaccctg cgcgggctga aagacgccag cgaactcaag caggcgctga aggattcgct
     4801 cagcgagctg atcgccccgc tgcaaatccc gctggacacc agcggtcacc agccgtgcgt
     4861 gatcatgctg gctggcgtca acggcgccgg caaaaccacc tcgatcggca agctggccaa
     4921 atacttccag agccagggca agagtgtgtt gctggccgcc ggcgacacct tccgcgccgc
     4981 cgcgcgcgaa cagctgatgg cctggggcga gcgcaataat gtcacggtga tcgcccagca
     5041 gggcggcgat tctgccgcag tgtgctacga cgccatccag gcggcagtgg cgcgcaagat
     5101 cgacatcgtg ctgg
//
LOCUS       DAOPYX010000218         5111 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_20622_length_5111_cov_10.381131, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000218 DAOPYX010000000
VERSION     DAOPYX010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 5111)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5111)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5111
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20622_length_5111_cov_10.381131"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..400)
                     /locus_tag="PKN55_10775"
     CDS             complement(<1..400)
                     /locus_tag="PKN55_10775"
                     /inference="COORDINATES: protein motif:HMM:NF017345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FdtA/QdtA family cupin domain-containing
                     protein"
                     /protein_id="HNX71769.1"
                     /translation="MLIQTVDFKVITDGRGSLISLEGGSADVPFDIQRVYYIFGVGEG
                     VVRGAHAHKRLRQVVVCMSGSCRMVLDNGLGVKEDIVLDTPTRGLLISSMTWREMHDF
                     SPDCVLVVLADSHYDESDYYRHYDDFLRAAQ"
     gene            complement(510..1484)
                     /gene="ylqF"
                     /locus_tag="PKN55_10780"
     CDS             complement(510..1484)
                     /gene="ylqF"
                     /locus_tag="PKN55_10780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003781668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HNX71770.1"
                     /translation="MSIQWFPGHMFKAKKELADKLSDIDVVIEMVDARLPMSSSNPML
                     AALIQHKPSLKILNKQDLADAAQTRAWLEWFAGQRNTRAIALDSGDGSPKQKILAACR
                     ELAPHRVDSVKPLRLVICGIPNVGKSTLINALTGRKVAKVGNEPAVTKAQQRIQLADD
                     VILFDTPGMLWPKIEHETCGEHLAASGAVGRNAMDEESVALALIATLQRRYPAELARR
                     YNLADVQAEDFVVLETIGRKRGAVRSGGHVDMQKAAEILLTDFRAAVIGRITLETPEE
                     WRDIAAEIERRRVIEREEQAQREAERKARRAGGRRARPGEDDEDDEQA"
     gene            complement(1481..1723)
                     /locus_tag="PKN55_10785"
     CDS             complement(1481..1723)
                     /locus_tag="PKN55_10785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HNX71771.1"
                     /translation="MTSHPAAFAPELLLVLVQQRMPFGKYQGRLLADLPLAYLTWFAR
                     QGWPPGQLGQCLALAYEIKHNGLSEVLAPLRKDPAE"
     gene            complement(1880..2710)
                     /locus_tag="PKN55_10790"
     CDS             complement(1880..2710)
                     /locus_tag="PKN55_10790"
                     /inference="COORDINATES: protein motif:HMM:NF012899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX71772.1"
                     /translation="MTDTSNTIKHIASPLARQTLQDGALTRPVASVRPIRLLPWLQVV
                     KIGGRLMDRGAEAILPLVDELRALLPEHRLLILTGAGLRARHVFSVGLDLGLPVGSLA
                     PLAASEAGQNGHILASLLAPEGVSYVEHPTIASQLAIHLQAARAVVGSAFPPYHHHEF
                     PESRIPMHRADTGAFLLADALGAAGLTIVEDVDGVYSCDPNGPDAAQAQLLAEASAAE
                     LAAQPGTLPFDRAMLDVMANARHIERVQVVNGLKSGRLTAALRGEHVGTLIRTGAHAG
                     "
     gene            complement(2721..>3443)
                     /locus_tag="PKN55_10795"
     CDS             complement(2721..>3443)
                     /locus_tag="PKN55_10795"
                     /inference="COORDINATES: protein motif:HMM:NF012899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX71773.1"
                     /translation="DFRILPDATVLKIGGQSVIDRGRAGLFPLVDEIVSARKDHKLLI
                     GTGAGTRARHLYSIAAGLNLPAGVLAQLGASVADQNAAMLGQLLAKHGIPVVGGAGLS
                     TVPLSIAEVNAVIFSGMPPYKLWMRPAAESVIPPYRTDAGCFLLAEQFGCKAMIYVKD
                     ENGLYTGNPKTDPHATFIPKISVDEMKAQGLQDSILEFPVLDLLKSARHIRQVQVING
                     LVPGNLTRALAGEHVGTIITAS"
     assembly_gap    3444..3543
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3714..>5111
                     /locus_tag="PKN55_10800"
     CDS             3714..>5111
                     /locus_tag="PKN55_10800"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012698091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003904 - deoxyribodipyrimidine
                     photo-lyase activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HNX71774.1"
                     /translation="MPRFARVLCWFRRDLRLSDHAALYHALKSADEVIPVFVFDRDIL
                     DVLPGDDRRLPFILACLHELQQALRAHGGELVTAHASAVEALPALAAQWGAEAVFAAR
                     DYEPAAIARDAAVRQRLAAQGVAWFDYKDQVIFECDEVLTQGGKPFSVFTPYKNAWLK
                     KLTPFYLQSYPVERYLPALARLPEQTLPTLDALGFADAAAQPLKITPGISGAQAALAD
                     FQRRLTHYQRLRDFPASKGPSYLSPHLRFGAVSIRLLAQLAWAQGDEGAQTWLNELIW
                     REFYQQILWHHPRVAEHAFKPEYDALAFDNDPELFAAWCEGRTGYPIVDAAMRQLAQT
                     GYMHNRLRMIVASFLVKDLLIDWRWGEAHFARLLLDFDLAANNGGWQWAASTGCDAQP
                     YFRIFNPVSQSEKFDAEGKFIRRYVPELAALDNKAIHAPWLAKRLPEDFRLGRDYPKP
                     IVDHATQRQRALALFG"
BASE COUNT          873 a         1588 c         1650 g          900 t
ORIGIN      
        1 cctgcgccgc gcgcaaaaaa tcgtcgtagt gacggtagta atcgctttcg tcgtaatggc
       61 tgtcggccag caccaccagc acgcaatccg ggctgaagtc gtgcatttcc cgccaggtca
      121 tgctgctgat cagcaggccg cgtgtggggg tatccagcac gatgtcttct ttcaccccca
      181 gaccgttatc cagcaccatg cggcagctgc cggacatgca caccaccacc tgacgcaggc
      241 gcttgtgcgc atgtgcgccg cgcaccacgc cttcacccac accaaagatg taatacaccc
      301 gctgaatatc aaacggcacg tcagcactgc cgccctccag cgagatcaac gagccgcgcc
      361 cgtcggtgat aactttaaaa tctacggttt gaatcagcat gcaaagcgtc cgctcagtgt
      421 gggccgtgcg cggccagggt caaaaaagag gtcagggccg atgcaatatg cgctgtcaga
      481 caggccccag gcctgacacg cgactgaact caggcctgct cgtcgtcctc gtcatcctca
      541 ccagggcgcg cgcgccggcc gccggcgcga cgcgccttgc gctcggcctc gcgctgcgcc
      601 tgttcttccc gctcgatcac ccgacggcgc tcgatttcgg cggcgatgtc gcgccattct
      661 tccggggttt ccagggtgat gcggccaatc accgccgcgc gaaaatcggt cagcaggatt
      721 tcagcggctt tttgcatgtc cacatggccg ccgctgcgca ccgcgccgcg cttgcggccg
      781 atggtttcca gcacgacaaa atcttccgcc tgcacatcgg ccaaattgta gcgacgggcc
      841 aattcggccg ggtagcggcg ttgcaaggtg gcgatcagcg ccagcgccac gctttcttcg
      901 tccatggcgt tgcggcccac tgcgccgctg gcggccaggt gctcgccgca ggtttcgtgt
      961 tcgattttcg gccacagcat gccgggggtg tcgaacaaaa tcacatcgtc ggccagctgg
     1021 atgcgctgct gcgccttggt caccgccggc tcattgccca ccttggccac cttgcgcccg
     1081 gtcagcgcat tgatcagcgt ggatttgccg acgttgggaa taccgcagat caccaggcgc
     1141 agcggcttga cgctgtccac ccggtgcggg gccagctcgc ggcaggcggc caggattttt
     1201 tgcttgggcg agccatcgcc gctgtccagg gcaatggcgc gggtgttgcg ctggccggca
     1261 aaccattcca gccaggcgcg ggtttgtgcg gcgtcggcca agtcctgctt attgaggatt
     1321 ttcaggctgg gcttgtgctg gatcagcgca gccagcatcg ggttggacga ggacatcggc
     1381 agacgggcgt ccaccatttc aatcaccacg tcaatatcgg acaacttgtc ggccagttcc
     1441 ttcttggcct tgaacatatg gccgggaaac cattggatac tcattcagcg gggtctttcc
     1501 gtagcggcgc gagcacttcg ctcaggccgt tgtgcttgat ttcataggcg agcgccaggc
     1561 attggcccag ctggcccggc ggccagccct ggcgggcaaa ccaggtgagg taagccagcg
     1621 gcaggtcggc cagcagccgc ccctgatatt tgccaaacgg catgcgctgt tgcaccagca
     1681 caagcagcag ttcgggggca aaggcggcag gatgggaggt catggccgat attgtagcgc
     1741 atccggccag gcagaatcgg cgcaaacaca cggaaaaaga acgagatggc gcgccggact
     1801 tgcgtcgggc gcgccagggc cggcgccgct caccccagcg tgacagcgtg gtgagcggga
     1861 cactcggcgg cgggaaggat cagccagcgt gggcgccggt gcggatcagt gtgccgacat
     1921 gttcaccgcg caaagccgcg gtcaggcggc cggacttgag gccattgacc acttgcacgc
     1981 gctcgatgtg gcgggcgttg gccatcacgt cgagcatggc gcggtcaaac ggcagcgtgc
     2041 ccggctgggc ggccagctcg gcggcggacg cctcggccaa cagctgcgcc tgggcggcgt
     2101 cggggccgtt cgggtcgcag gaatacacgc cgtccacgtc ttcgacaatg gtcagcccgg
     2161 cagcgcccag cgcgtcggcc agcaggaagg cgccagtgtc ggcgcggtgc atggggatgc
     2221 gcgactcggg gaattcatgg tggtggtagg gcgggaaggc gctgccgacc acggcgcgcg
     2281 cggcttgcag gtggatggcc aactggctgg cgatggtcgg atgctcgacg taagacacgc
     2341 cttccggggc cagcagcgag gcaaggatat ggccgttctg gccggcttca ctggccgcca
     2401 gcggcgccag cgagcccacc ggcaggccca gatccaggcc gacgctgaac acatggcggg
     2461 cgcgcaggcc ggcgccagtg agaatcagca ggcggtgttc cggcaagagc gcgcgcagct
     2521 cgtccaccag cggcaggatg gcttcggcgc cacggtccat caggcggccg ccaatcttga
     2581 ccacttgcag ccagggcagc aggcgaatcg ggcgcacgct ggccaccggg cgggtcaatg
     2641 cgccgtcctg caaggtttgg cgggccagcg gcgaggcgat gtgtttgatg gtgttggagg
     2701 tgtcagtcat ggtttctgcc tcagctcgcg gtgatgatgg tgccgacatg ctcgccggcc
     2761 agggcgcggg tcaggttgcc gggaaccagg ccattgatga cctgcacctg acggatgtgg
     2821 cgggcggatt tcagcaaatc cagcactggg aattccagga tggaatcttg caggccctgg
     2881 gctttcattt cgtccaccga gattttggga ataaacgtcg cgtgcgggtc ggttttcggg
     2941 ttgccggtgt acaggccgtt ttcgtctttc acgtagatca tcgccttgca gccaaactgc
     3001 tcggccagca aaaagcagcc ggcgtcggtg cggtagggcg gaatcacgct ctcggccgcc
     3061 gggcgcatcc acagcttgta cggcggcatg ccgctgaaaa tcaccgcgtt cacctcggca
     3121 atcgacaagg gcacagtgga cagcccggcg ccgcccacca ccggaatgcc atgcttggcc
     3181 agcaactggc cgagcatggc ggcgttctgg tcggccaccg acgcgcccag ctgggcgagc
     3241 acgccggccg gcagattgag cccggcggcg atcgaataca aatggcgggc gcgggtgccg
     3301 gcgccggtgc cgatcagcag cttgtggtct ttgcgggcgc tgacgatttc gtccaccagc
     3361 ggaaacaggc cggcgcggcc acggtcgatc acgctctggc cgccgatttt cagcacggtg
     3421 gcgtcgggca gaatgcgaaa gtcnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3541 nnnagcgcag cataggcgtg agcgttgtat agattcaacc gcataactac ccgtaattca
     3601 ttaagcaaaa cgaatgccac gcccagcgtg atacagcgcg tcactgaacg cacagggaac
     3661 gcgcaggcgc tggtttgtcc agtacaattc agtttatttg ccgagccgcc gccatgcccc
     3721 gttttgcccg agtgttgtgc tggtttcgcc gcgacctgcg cctgagcgac catgctgcgc
     3781 tgtatcacgc gctgaaatct gcggatgaag tgattccggt gtttgtgttc gaccgcgaca
     3841 ttctcgacgt cctgccaggc gacgaccgtc ggctgccgtt cattctggct tgcctgcatg
     3901 agctgcagca ggccttgcgc gcgcacggcg gcgagctggt gacggcgcac gccagcgccg
     3961 tggaggcgct gccggcgctg gccgcgcagt ggggcgccga ggcggtgttc gctgcgcgtg
     4021 attacgagcc ggccgccatc gcccgcgacg ccgccgtgcg ccaacggctg gcggcgcagg
     4081 gcgtggcgtg gttcgactac aaagaccagg tgatttttga gtgtgacgaa gtgctcaccc
     4141 agggcggcaa gccgttttcg gtgttcaccc cgtataaaaa cgcctggctg aaaaaactga
     4201 cgccgtttta cctgcaaagc tatccggtgg agcgttattt gccggcgctg gcgcggctgc
     4261 cggaacagac tttacccaca ctggacgcac tcggttttgc cgacgccgcc gcgcagccgc
     4321 tgaaaatcac ccccggcatc agcggcgcgc aggcggcgct ggcggatttt cagcgccggc
     4381 tgacccacta ccagcggctg cgggattttc ccgccagcaa ggggccgtcg tatctgtcgc
     4441 cgcatctgcg ctttggcgcc gtgtccatcc gcctgctggc gcaattagcc tgggcgcaag
     4501 gcgatgaagg cgcgcaaacc tggctgaatg agctgatctg gcgcgagttt taccagcaga
     4561 ttctctggca tcacccacgg gtggccgagc atgcgttcaa gcccgaatat gacgcgctgg
     4621 cgtttgacaa cgaccccgag ctgttcgccg cctggtgcga aggccgcacc ggctacccga
     4681 ttgtggatgc cgccatgcgc cagctggcgc aaaccggcta tatgcacaac cggctgcgca
     4741 tgattgtcgc cagctttttg gtgaaagacc tgctgattga ttggcgctgg ggcgaggcgc
     4801 attttgcccg gctgctgctg gattttgacc tggcggccaa taacggcggc tggcagtggg
     4861 cggcgtccac cggctgcgac gcccagccgt atttccgcat tttcaacccg gtcagccagt
     4921 cggagaaatt cgacgccgaa ggcaagttta tccgccgcta cgtgccagaa ctcgccgcgc
     4981 tggacaataa agccatccac gcgccctggc tggccaaacg cctgccggaa gactttcgcc
     5041 tggggcgcga ctaccccaag ccgattgtgg accacgccac ccagcgccag cgcgcgctgg
     5101 ccttgtttgg c
//
LOCUS       DAOPYX010000219         5099 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_20682_length_5099_cov_8.790444, whole genome shotgun sequence.
ACCESSION   DAOPYX010000219 DAOPYX010000000
VERSION     DAOPYX010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 5099)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5099)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5099
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20682_length_5099_cov_8.790444"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(2..2857)
                     /locus_tag="PKN55_10805"
     CDS             complement(2..2857)
                     /locus_tag="PKN55_10805"
                     /inference="COORDINATES: protein motif:HMM:NF024642.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HNX71775.1"
                     /translation="MRILEYIDLDTSGVAKTYRKVATAIAAGDFRAAQVKKLSNLTHG
                     KFYRARLDDANRLLFTLVRHGDEVCALMLEVIANHDYDKSRFLRGASIDESKIPDCDL
                     AQASAEAQPLRYLHPEHAGVHLLDKPISFDDAQEAIYRQPAPLVLVGSAGSGKTALTL
                     EKLKHAEGEVLYVTHSAFLAQSARDLYYANGFEHTGQEAVFLSYREFIETIQAPPGRE
                     AQWRDFAAWFERMRQSVRGVDAHQAFEEIRGVIAAAAEGVLSREAYRELGVRQSIFAP
                     EQRDLVYDVFEKYRSWLAESRLFDLNLIAQDWLARAQPRYDFVVIDEVQDITPVQLAL
                     VLKTLKKPGHFLLCGDSNQIVHPNFFSWAQVKTLFWHDATLAERQELRVLAANFRNGQ
                     ETTRVANQLLKIKQCRFGSIDRESNYLVQAVGGEVGAVSLLGDKDAAKKQLDQQIRQS
                     TQFAVLVMRDEDKAEARKYFSTPLLFSIHEAKGLEYENIVLYRFVSDHRAEFTEITQG
                     VNPADLLAEQLDYRRAKDKSDKSLEIYKFFVNALYVALTRAIRNVYLIESDTQHPLFE
                     LLEVKADGPVKVEARQASLEDWQKEARKLELQGKQEQADAIRNQILKQTPPPWTVQDQ
                     PHTEQLLTKVFRDNAPGSKHRQPLYEQAVCYDLPVLADFLQEHGKFQAANTFYTQRSL
                     LVRKNYMAYFSPNFKDILRQCDKHGVEHRLPVNLTPLMAATAAGNLPLVDALLERGAS
                     LDAQDTFGCTALHWAMQEAFRDPKYAKGPFAALYERLAPAWVDVNTGERLVRIDRHLT
                     EYFVFQTLWALFRSRFTHFQRRPYAAFDSQTLIDAWEALPANVVRPERKKRQHLNHVL
                     SRNEIMRDYAYNRALFQRVRQGWYQFNPQLLVRDKTADGAWMPIYTALNLPFTAQFSD
                     FPVWNMIDTYLAAADLPPRAELILPWKSTSTLRYGWE"
     gene            complement(3010..4086)
                     /locus_tag="PKN55_10810"
     CDS             complement(3010..4086)
                     /locus_tag="PKN55_10810"
                     /inference="COORDINATES: protein motif:HMM:NF013798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macro domain-containing protein"
                     /protein_id="HNX71776.1"
                     /translation="MITFTQGNLLEARTEALVNTVNTVGVMGKGIALMFKERFDENFR
                     RYAAACKAKEVQTGKMFVTQVHELDGPRWIVNFPTKQHWRAPSRMEWVLEGLQDLRCF
                     LIEQQIQSIAIPPLGAGNGGLEWADVREQIELALGDLDIDILVFEPTKQYQNVAKRAG
                     VEKLTPARALIAELVRRYWVLGMECSMLEIQKLAWFLERAIERYNPGDNPLNLQFTPH
                     KYGPYANRLDHLLNNLDGSYLHSETRISDASPLDIIWFDDGRKAFVQTYLKNEAKAYT
                     QALEFTAALIDGFESPFGMELLATVDWLLAREGVAPNVSALREGLRHWRGGSGAAARK
                     DRLFDDRALGIALERLTQPVTALA"
     gene            complement(4086..4730)
                     /locus_tag="PKN55_10815"
     CDS             complement(4086..4730)
                     /locus_tag="PKN55_10815"
                     /inference="COORDINATES: protein motif:HMM:NF025840.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4433 domain-containing protein"
                     /protein_id="HNX71777.1"
                     /translation="MLYTSLNPEKALIWRIVHRDNLPWILDNGLYCGHSSVKAPSWVS
                     IGNPELIDKRAHHPVRLPPGGLLNDYVPFYFTPFSPMLRNIHTGWGGIQKRPNEEIVI
                     LVSSLHHIARLGLPFLFTDSHAYYQWADYYSNLADLDKIDWPLLQRRDFKRDPEDPAK
                     FERYQAEALIHQHLPVGGLLGIVCYTEPLKQRIEQEIQAHNLSLPVYARTGWYF"
BASE COUNT         1074 a         1423 c         1462 g         1140 t
ORIGIN      
        1 cttattccca gccgtatctc agcgtcgaag tcgatttcca aggcaaaatc agctcggcgc
       61 gcgggggcag gtcggcggcg gccaggtatg tgtcgatcat gttccagacg gggaagtcgg
      121 aaaactgggc ggtaaacggc aggttcagcg cggtgtaaat cggcatccag gcgccgtctg
      181 cggttttatc gcgcaccaag agctgcggat taaactgata ccagccttgt cgcacgcgct
      241 ggaacagcgc gcggttatag gcataatcgc gcatgatttc gttgcgcgac agcacgtgat
      301 tcaggtgctg gcgcttttta cgttcgggcc gcaccacatt ggcgggcagt gcttcccagg
      361 cgtcgatcag ggtttgcgag tcaaacgcgg catacgggcg gcgctgaaaa tgggtgaagc
      421 ggctgcggaa caaggcccac agtgtctgaa agacaaagta ttcggtcaga tgccggtcaa
      481 tgcgcaccag gcgctcgccc gtgttgacgt ccacccaggc tggggccaga cgttcgtata
      541 gcgcggcaaa cggccctttg gcatatttgg ggtcacgaaa tgcttcttgc atggcccagt
      601 gcagggcggt gcagccaaat gtgtcctggg cgtccaggct ggcgccgcgc tccagcaggg
      661 cgtccaccag cggcagattg cctgcggcgg tggcggccat cagcggggtg agattcaccg
      721 gcagccggtg ttccacgcca tgtttgtcgc attggcgcag aatgtctttg aagtttggcg
      781 aaaaatacgc catgtagttt ttgcgaacca acagcgatcg ttgcgtgtaa aacgtgttcg
      841 ccgcttgaaa tttgccatgt tcctgcaaaa aatcggccag cacgggcaag tcatagcaga
      901 cggcctgctc gtacaagggc tgccggtgtt tgctgcccgg cgcgttatcg cggaacacct
      961 tggtcagcag ttgttcggtg tgcggctgat cctgcaccgt ccacggcggc ggcgtttgct
     1021 tgagaatctg gttgcggatg gcatcggctt gttcttgttt gccttgcagc tccagtttgc
     1081 gcgcttcttt ttgccagtct tctaggctgg cttgcctggc ttccaccttg accgggccgt
     1141 ctgctttaac ctccagcaat tcaaatagcg ggtgctgggt gtcggattcg atcagataca
     1201 cattgcggat ggcgcgggtc agcgccacgt acaacgcatt gacaaaaaac ttgtaaattt
     1261 ccagcgattt gtcgcttttg tctttggcgc ggcggtaatc cagttgctcg gccagcaagt
     1321 cggccgggtt taccccctgg gtgatttcag taaattcggc gcggtggtcg gagacaaagc
     1381 gataaagcac aatgttttca tattccagcc ccttggcttc gtgaatcgaa aacagcagtg
     1441 gggtagaaaa atacttgcgc gcctcggcct tgtcttcatc gcgcatcacc agcacggcaa
     1501 actgggtgga ttggcgaatc tgctggtcga gttgtttttt ggcggcgtct ttatcgccca
     1561 gcaggctgac cgcgcccacc tcgccgccca cggcttgcac caggtaattg ctttcacggt
     1621 caatcgagcc aaaccggcat tgcttgattt tcagcagctg gttggccacc cgcgtggttt
     1681 cctggccatt gcgaaaattg gccgccagca cgcgcagttc ctggcgttcg gccagcgtgg
     1741 cgtcatgcca gaacagggtt ttgacttgcg cccaagaaaa gaaattggga tgcacaatct
     1801 ggttggagtc gccgcacagc aaaaagtggc cgggtttttt cagggttttc aacaccagcg
     1861 ccaattgcac cggggtgatg tcctgcactt cgtcgatcac cacaaagtca tagcgcggct
     1921 gtgcgcgggc cagccaatcc tgcgcgatca gattcaggtc aaacaggcgt gattccgcca
     1981 gccagctgcg gtatttttca aacacgtcgt acaccagatc gcgctgttcc ggggcaaaaa
     2041 tcgattgacg caccccaagc tcgcgatagg cctcacggct gagcacgccc tcggccgctg
     2101 cggcaatgac gccgcgaatt tcttcaaacg cctgatgggc gtccaccccg cgcacactct
     2161 ggcgcatgcg ctcaaaccag gcggcaaagt cgcgccattg cgcttctcgc cccggcggcg
     2221 cctgaatggt ttcgataaat tcccgatacg acagaaacac cgcttcctgg ccggtatgct
     2281 caaaaccatt ggcgtagtac aggtcgcggg cgctttgcgc caaaaacgcc gagtgggtga
     2341 catacagcac ctcgccttcc gcgtgtttca gtttttccag cgtcaacgcg gttttgccgc
     2401 tgccggcgct gcccaccaac accaagggcg ctggctggcg ataaatggcc tcttgcgcat
     2461 cgtcaaacga aatcggctta tccagcagat gcaccccggc atgttccggg tgcaaatagc
     2521 gcagcggctg cgcctcggcg cttgcctggg ccagatcgca atccggaatc ttgctttcat
     2581 caatgctggc gccccgcaaa aaacgcgact tgtcgtagtc atgattggcg atgacttcca
     2641 gcatcagcgc gcagacttca tcaccatgcc gcaccagcgt aaacaacagc cggttggcgt
     2701 catccagccg cgcccgataa aacttgccgt gggtcagatt ggacagtttt ttgacttgtg
     2761 ccgcccgaaa atcaccggca gcaatggcgg tggccacctt gcggtaagtt ttggctaccc
     2821 cggaggtgtc cagatcgatg tattcaagaa tgcgcatggt attaggatgt ggaagaaatg
     2881 tggatgtgca gggccggtca gtcagcgttc tgtccttcga cattgggaaa ggaggcagcg
     2941 ctcagaaaat acggatcact cgggatgcaa caggtagcca gggccgcttc gctgggtttt
     3001 gtgagaacgc taagccagtg ctgtcacagg ttgcgtcagc cgctcaaggg caatgcccag
     3061 tgctcggtca tcgaagaggc ggtccttacg ggcggcagcg cccgaaccac ctcgccaatg
     3121 acggagccct tcacgcaggg cagaaacgtt gggcgccacg ccttcgcggg ccaggagcca
     3181 gtccacggtc gccagcagtt ccatgccaaa gggtgattca aagccgtcaa tcagtgctgc
     3241 tgtgaattct agcgcttgcg tgtaggcctt ggcttcgttc tttagatagg tctgaacaaa
     3301 ggctttgcgt ccatcgtcga accagattat atccaacgga ctggcatcgc tgatgcgcgt
     3361 ttcggagtgc aagtagctgc catccagatt gttcagcagg tggtcgagcc ggttggcgta
     3421 ggggccgtat ttgtgtggcg taaactgtag gttcaatggg ttatcgccgg ggttgtaacg
     3481 ctcaatggcg cgttccagaa accatgccag cttctgaatc tccagcatgc tgcactccat
     3541 gcccaacacc cagtaccggc gcaccagttc ggcgatcagc gcacgggcag gcgtcagctt
     3601 ttctacgcct gcacgcttgg ccacattttg gtattgtttg gtgggttcga acactagaat
     3661 gtcgatgtcc agatcgccca atgctaattc gatctgctca cgcacatcag cccattccaa
     3721 accaccgtta cccgcaccca gcggcgggat agcgatggac tggatctgtt gctcaatcaa
     3781 gaagcaccgc agatcctgca agccttccag cacccactcc atgcgcgagg gcgcacgcca
     3841 gtgctgctta gtggggaaat tgacgatcca gcgcggcccg tctagttcgt gcacctgcgt
     3901 tacaaacatc ttgccggtct gtacctcctt agctttacaa gcggcggcat agcggcggaa
     3961 gttctcatca aagcgttctt tgaacatcag tgcgatgccc ttgcccatca ccccgacggt
     4021 attgacggtg ttgaccagcg cctcggtgcg ggcctccaac aaatttcctt gagtgaatgt
     4081 aatcatcaga agtaccaccc tgttctggca taaaccggca ggctcagatt atgggcctga
     4141 atctcctgtt cgatgcgttg tttcaatggt tctgtgtaac acacgatgcc gagaagtccg
     4201 cccaccggca agtgttggtg aatcagtgcc tcggcctgat agcgctcgaa cttggcaggg
     4261 tcttccgggt cacgcttgaa gtccctgcgc tgtagcaaag gccagtcgat tttatcgagg
     4321 tcagccaagt tcgaatagta atccgcccac tgataatagg catggctgtc agtgaacaaa
     4381 aacggcaagc ccagcctagc aatgtggtgt aggctggaca ccaagatcac gatttcttcg
     4441 tttggccgct tctggatgcc gccccatccg gtatggatgt tgcgcagcat cggcgagaag
     4501 ggtgtgaagt agaacggcac atagtcgttc agcaagccac ctggcggtag ccgtaccgga
     4561 tgatgagcgc gcttgtctat aagctccggg ttgccgatac tcacccagct tggcgccttc
     4621 accgagctgt ggccgcaata caagccattg tccagaatcc agggtagatt gtcacggtga
     4681 acaatccgcc agatcagtgc tttttctgga ttgaggctgg tatagagcat cagagaaact
     4741 tccttggatt tacatctggc atgtctcaaa caccaagtgc aacagagtca atgtttagcg
     4801 tgaaacaccc cgatttttgg gcgcaaaaaa gcatggcgaa caatcgaaca atcaagacgc
     4861 gatgcgagca gcaaagggtg gggacagtga gttcgttttt attctatggg gtagcgcgtc
     4921 acctcgcaat ggacgttaga gccagggcaa tcgcatcaac gcctttatca tgtacttggc
     4981 cggcatttgc ccacaatcag caagccagat actgtccaca ccctatctac ctgtaggttt
     5041 tttggacgga atcatggaaa aagaaggccc gcagcggctg gccgggtgtt catttcatg
//
LOCUS       DAOPYX010000220         5077 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_20828_length_5077_cov_7.892075, whole genome shotgun sequence.
ACCESSION   DAOPYX010000220 DAOPYX010000000
VERSION     DAOPYX010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 5077)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5077)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5077
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20828_length_5077_cov_7.892075"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..462
                     /locus_tag="PKN55_10820"
     CDS             <1..462
                     /locus_tag="PKN55_10820"
                     /inference="COORDINATES: protein motif:HMM:NF013331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX71778.1"
                     /translation="PELILPEVPFPSLRVIEALGEHGLRQLVAQHHALLRQSAIGHLF
                     AQDAAQFAQLVERVADFVVEACGGAAQYTPAHGHTCMRTRHFPFTIDEAAREVWLTLL
                     WQALADCAAPAAVREEYWAWMEPFSLRMINRRTTKAQPARWAYAEMAARHA"
     gene            455..1312
                     /locus_tag="PKN55_10825"
     CDS             455..1312
                     /locus_tag="PKN55_10825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX71779.1"
                     /translation="MLKAWRSGIGLLALAWATWAGAQPPAPSATARPVVRVAVVGGLL
                     QSGVWPRLADKAAVGAGLRVDTVAAAPKEGIVPVFARGDADLMLIHGSDESYALLAAG
                     LAAPLRAWAANEHVLVGPADDPAGVAQAADGAAAMARIVAADAPLLAFRDPGSFSIAQ
                     ALFRRAGVRPGPRQQLYDDAESPQSVLASAARQGAYAVVGHIPVASGKMPSHGLKVLL
                     HGDPAMRRVYVLVEPGPAHPANAERRRLARRLADYLLSARGQADLRAADREAGGPWVF
                     ALTTGGPAR"
     gene            complement(1544..2548)
                     /gene="ppk2"
                     /locus_tag="PKN55_10830"
     CDS             complement(1544..2548)
                     /gene="ppk2"
                     /locus_tag="PKN55_10830"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011135272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006793 - phosphorus metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2"
                     /protein_id="HNX71780.1"
                     /translation="MTDSLENKTPATATATAARTRHRSQATTDKAHQQQEHAAEEIRA
                     ISEAPVAVQVATAPHGSDEDSSSAPLPADYPYKTRIRRADYERQKQALQVELLKVQNW
                     VKETGQRIVILFEGRDAAGKGGTIKRYMEHLNPRGARVIALEKPTEIERGQWYFQRYI
                     QHLPSAGEMVFFDRSWYNRAGVERVMGFCTPSEYLEFMRQTPELERMLVNSGIRLFKY
                     WFSVNREEQLRRFISRRDDPLKHWKLSPIDIQSLDKWDDYTAAKKAMFFHTDTADAPW
                     TVIKSDDKKRARLNCLRHFLSTLDYPNKDPKVACPPDPLLVGNASHVLEADETHIDRF
                     "
     gene            3110..4813
                     /locus_tag="PKN55_10835"
     CDS             3110..4813
                     /locus_tag="PKN55_10835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012696880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HNX71781.1"
                     /translation="MSHTDKAPGQEPVIANFIRNIIDDDLAQGKHSSLLTRFPPEPNG
                     YLHVGHAKSICLNFGLVQGEAAYPGQCNLRFDDTNPEKESTEYAESIQADVRWLGFEW
                     AGEVKWASDYFDALYQYAEALIQAGKAYVCELNAEQMREYRGSLKEPGKNSPYRERSV
                     AENLDLFRRMKAGEFPDGSKTLRLKIDMASPNINLRDPVIYRIRRAHHIRTGDAWCIY
                     PMYDYTHCISDALEGITHSLCTLEFEDHRPLYDWVLDNIDIPCHPRQYEFSRLELFYT
                     VTSKRKLNQLVTEKLVAGWDDPRMPTIAGMRRRGYTPEGIRLFARRIGISKSDNHVDM
                     SVLEGAVREELEHSAPRVMAVINPLKVTLTNYTEGVTAARSAGFHPNHPEFGEREVPL
                     SAEIWIERDDFSDNPPAGWQRLTPGGEVRLRYSYVIKCDDVVRDAAGEVIELRCSIDH
                     DTLGKNPVGRKVKGVIHFVAAAHALPIEVRLYDRLFSVPRPDAMKDEAGAEIDFKTFI
                     NPDSLSVVTGYAEPALAQAQPEARYQFERIGYFCADRHEHSASRPVFNRTVGLKDTWS
                     K"
BASE COUNT          843 a         1625 c         1681 g          928 t
ORIGIN      
        1 ccggagctga ttttgccgga agtgccgttt ccgtccctgc gggtgatcga agcgctgggc
       61 gaacacggcc tgcgccagtt ggtggcgcag catcacgcgc tgctgcgcca gagcgccatc
      121 ggccatctgt ttgcccagga tgcggcgcag tttgcccagt tggtcgagcg ggtggccgat
      181 tttgttgtcg aggcctgtgg cggcgcggcg caatacacgc cggcgcacgg ccacacctgc
      241 atgcgcaccc ggcattttcc gttcaccatc gacgaagcgg cgcgcgaagt ctggctgacc
      301 ctgctgtggc aggcgctggc tgactgcgcc gcgccggcgg cggtgcgcga ggaatattgg
      361 gcgtggatgg agcccttctc gctgcgcatg atcaaccggc gcaccaccaa ggcgcagccg
      421 gcgcgctggg cctatgccga gatggcggcg cgtcatgctt aaggcctggc gcagcggcat
      481 cggcctgctg gcgctggcct gggcaacatg ggcgggcgcg cagccgccag cgccaagcgc
      541 cacggccagg cccgtggtgc gggtggcggt ggtcggcggc ttgctgcaaa gcggggtgtg
      601 gccacggctg gccgacaagg cggctgtggg cgccggcctg cgggtggaca ccgtggcggc
      661 ggcgcccaag gagggcattg tgccggtgtt cgcccgtggc gacgccgacc tgatgctgat
      721 tcatggcagc gacgaaagct atgcgctgct ggctgccggc ctggcggcgc cgctgcgcgc
      781 ctgggcggcc aacgagcatg tgctggtcgg ccctgcggat gacccggccg gcgtggcgca
      841 ggcggcggat ggcgcggcgg cgatggcgcg gattgttgcc gccgacgcgc ccttattggc
      901 gtttcgcgac cccggcagct tcagcattgc ccaggcgctg ttccgccgcg ctggcgtgcg
      961 ccctggcccg cgccagcaac tgtacgacga cgccgaatcg ccacagtccg tgctggccag
     1021 cgccgcgcgt cagggcgctt atgccgtggt cggacatatt ccggtggcca gcggcaagat
     1081 gcccagccat ggcctgaaag tgctgctgca tggcgatccg gccatgcgcc gggtgtatgt
     1141 gctggtcgaa cccggcccgg cgcacccggc caatgccgag cgccgccgcc tggcgcggcg
     1201 gctggcggat tatctgctga gcgcgcgcgg ccaggccgat ttgcgcgcgg cagaccgtga
     1261 ggccggcggg ccgtgggtgt ttgcgctgac aacaggcggc ccggcgcgtt gacagacaac
     1321 cggtgcggcc gtgatgcagc aagcgggcgc acaacggctg tcccgccagc catgaagcgc
     1381 cttgctgtgg taggtttttc gccgccccac gtgatcacgg cctgtccccc atgcggtaag
     1441 acatgggcaa caggccgtca gatgatttcc gagccgagtg cgcggggcgt ggcgcgggcg
     1501 gggatgactc ccccgcccga tttttttgcc tgcgcgcagc gcgtcagaag cggtcgatat
     1561 gggtttcgtc ggcttccagc acgtgcgagg cattgcccac cagcagcgga tccggcgggc
     1621 aggccacttt cgggtctttg ttcgggtagt ccagcgtgga caggaagtgg cgcagacagt
     1681 tcaggcgtgc gcgcttcttg tcgtcggact tgatcaccgt ccacggcgca tcagcggtgt
     1741 cggtgtggaa gaacatcgcc tttttggcgg cggtgtagtc gtcccatttg tccagcgact
     1801 ggatgtcgat cggcgagagc ttccagtgct tgagcggatc gtcgcggcgc gagatgaagc
     1861 ggcgcagctg ctcttcgcgg ttcaccgaga accagtattt gaacagacgg atgccgctgt
     1921 tcaccagcat gcgctccagc tcgggggtct ggcgcatgaa ttccaggtat tcgctggggg
     1981 tacaaaagcc catcacccgc tcaacgccgg cgcggttgta ccaggagcgg tcgaagaaca
     2041 ccatttcgcc agccgacggc aggtgctgga tatagcgctg gaagtaccac tggccacgtt
     2101 cgatttcggt gggtttttcc agggcgatca cccgcgcgcc gcgcggattg aggtgttcca
     2161 tatagcgctt gatggtgccg cccttgccgg cggcatcgcg gccttcaaac agaatcacaa
     2221 tgcgctggcc ggtttctttc acccagtttt gcaccttgag caattcaact tgcagcgctt
     2281 gtttttgccg ctcgtaatca gcgcggcgga tgcgggtctt gtaggggtag tcggccggca
     2341 gcggggcgga cgagctgtct tcgtcgctgc cgtgcggggc ggtggccacc tggacggcga
     2401 cgggcgcctc gctgatggcg cggatttctt cggcggcgtg ttcctgttgc tggtgcgcct
     2461 tgtccgttgt ggcttgtgac cggtgacgag tgcgcgcggc cgtcgcggta gcggtggcgg
     2521 gggtcttgtt ttccagcgaa tcggtcattg cacaaccttc ctttcttggg atttcagaaa
     2581 tggcgaaatt caagcgtgac caggcctggc gtggcaggtc ggcgggctgc gcgcgggtgg
     2641 gcggcggcct gtcggcccgt cccgtcggtc tgttgcgcgc ctggggcact catcccgttg
     2701 gccaagccgg gctgcgcagc gtggggggat gacggctcgg tcgtccgttt catgccgcag
     2761 caagcccagg cccaagcctg gtgtgacgcc ccgttctggc ggatcagccg gttcgggatg
     2821 ggtttggaaa gcgccatgca ggcgctttca agagtcgccc tccagcaggt ctggctggcc
     2881 cgggcgactc gcctcatcca ctatcctatg cctttcaaat aaccctgtcg acagtattgc
     2941 aattcagtga cgctgttttg ccgggcatga ccagatgcac agcgccagtt tcagcggttt
     3001 tgggtagaat tgcaggttaa accgcgcgcc ctggacgcac gccggcctgt ttgggcctgg
     3061 ccgaatccgc cccgggcgcg cctttgtccc tttcatgacg cgagccacca tgagccacac
     3121 tgataaagcc cccggccagg agccggtgat cgccaacttc atccgcaaca tcatcgacga
     3181 cgacctcgcg cagggcaaac acagcagcct cctcacccgt tttccgcctg agcccaacgg
     3241 ctatctgcac gtgggtcacg ccaaatccat ctgtctgaac tttggcctgg tgcagggcga
     3301 agccgcctac cccggccagt gcaatctgcg ctttgacgac accaacccgg aaaaagaaag
     3361 caccgaatac gccgaatcca tccaggccga cgtgcgctgg ctgggctttg agtgggccgg
     3421 cgaggtgaaa tgggcgtcgg attattttga tgcgctctac cagtacgccg aggcgctgat
     3481 tcaggccggc aaggcctatg tgtgcgagct caacgccgag caaatgcgcg aatatcgcgg
     3541 ctcgctgaaa gagccgggca aaaacagccc ctaccgcgag cgcagcgtgg cggaaaacct
     3601 ggacctgttc cgccgcatga aggccggtga atttccggac ggcagcaaaa ccctgcgcct
     3661 gaaaatcgac atggcctcgc ccaatatcaa tctgcgcgac ccggtgatct accgcatccg
     3721 ccgcgcccac cacatccgca ctggcgatgc ctggtgcatc tacccgatgt acgactacac
     3781 ccactgcatc tcggatgcgc tggaaggcat cacccactcg ctgtgcacgc tggagttcga
     3841 agaccaccgt ccgctgtacg actgggtgct ggacaatatc gacatcccct gccacccgcg
     3901 ccagtatgaa ttctcccgcc tggagctgtt ctacaccgtc acctccaagc gcaagctcaa
     3961 ccaattggtg accgaaaaac tggtggccgg ctgggacgac ccgcgcatgc ccaccatcgc
     4021 cggcatgcgc cgccgtggct acaccccgga aggcatccgc ctgttcgccc gccgcatcgg
     4081 catctccaaa tccgacaacc atgtggacat gagcgtgctg gaaggcgccg tgcgcgaaga
     4141 gctggagcac agcgcgccgc gcgtgatggc ggtgatcaac ccgctgaaag tcacgctgac
     4201 caactacacc gaaggcgtca ccgccgcgcg cagtgccggt tttcatccca accacccgga
     4261 atttggcgag cgcgaagtgc cgctgtcggc cgaaatctgg atcgaacgcg atgatttctc
     4321 cgacaacccg ccagccggct ggcaacggct gaccccgggc ggcgaagtgc gcctgcgcta
     4381 cagctatgtg atcaaatgcg acgacgtggt gcgcgacgcg gccggcgaag tgatcgaact
     4441 gcgctgctcg atcgaccacg acaccctggg caaaaacccg gtgggccgca aggtgaaggg
     4501 cgtgattcat tttgtcgccg ccgcccacgc cctgccgatt gaagtgcgcc tgtacgaccg
     4561 cctgttcagc gtgccgcgcc cggacgccat gaaagacgaa gccggcgccg aaatcgactt
     4621 caaaaccttc atcaacccgg attcgctcag cgtggtcacc ggctacgccg aaccggcgct
     4681 ggcccaggcg cagccggaag cccgctatca gttcgagcgc atcggttatt tctgcgccga
     4741 ccgccacgaa cacagcgcca gccgcccggt gttcaaccgc acggtggggc tgaaggatac
     4801 ctggagcaag taagcgcggc tcacaaaacc tgcctggcgt tgtgcgcgac cttgctgtac
     4861 gactggtact gtctgtggtc gcgtgcccag ccaggaacgc tgcgctgagt tttgtgcgcg
     4921 acgcgcggcc gcgggcgctt cacgggtgga gcatgtggcc cgtctgactt tggtcggtat
     4981 tctggccgca gctggcgtat ttatcttgaa tcaaagataa aagcgccagc tgcggctttg
     5041 ttctgctacc tcaccagcag cgccctatca tcatgac
//
LOCUS       DAOPYX010000221         4941 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_21673_length_4941_cov_8.784896, whole genome shotgun sequence.
ACCESSION   DAOPYX010000221 DAOPYX010000000
VERSION     DAOPYX010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 4941)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4941)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4941
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21673_length_4941_cov_8.784896"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            313..1176
                     /locus_tag="PKN55_10840"
     CDS             313..1176
                     /locus_tag="PKN55_10840"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNX71782.1"
                     /translation="MSATHSPRAWLALHFTVLLFGCAGLFGHALPVSPWVLVFGRTLF
                     AALSLALCLLALRRPLRPRTGEGGRLALAAGACLALHWVTFFQAIQVGGVAAGLLGFA
                     AFPAAVVALDSLGQRRWPAWRDVAMVGLVSVGLVLVTPLSAEGAAVREGVLWGVLSAL
                     LFAVLALLNRRSASGDPLRLAGLQNAAAALWLLPLAVGQLPALSLRDWLGLAVLGVVF
                     TALAHALFLFSLRVLPAQRVAVVTALEPVYGMAFVWLLFAQVPDARMWLGGAVIVLAT
                     LWASLGKASKH"
     gene            complement(1367..3580)
                     /locus_tag="PKN55_10845"
     CDS             complement(1367..3580)
                     /locus_tag="PKN55_10845"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HNX71783.1"
                     /translation="MIRRLLLSALIALGLLAPGAAPAADAPPRVGVVLGGGGARGFAH
                     LGVLAELERLRIPIACMAGTSAGALIGGFYASGQSLAHIRATFERTDWDRVLSGQLNR
                     ADLPFKRKRDDYRNYFDLLTGFRDGSLRLPRAAVSSQHIDQLMRELGGNVLVDSFAQL
                     PIPFQAVATDLLTGDAYVFDHGDLAIAQRASMAVPGLFEPVELDGRLLVDGGMARQLP
                     VENLQGPNGCADVIIAIDVGTPSLKREDIRSFVDVLAQYTQIGVLQNVRQQEQKLDLN
                     RDVLIRPMLGQYTAADFKAHHELAELGRLAVQPHVQALSRYSVSEADYARWQSTRLAL
                     RPTAPHIDTVTIAAGSDMAPAASLAALKPATGQPLDSAHLSRQLDQAYASGDFERVGY
                     RLWREDNRTTLELMPQERSVAPNYLRMGLGLTSGTDGQNAFNLLFSHHRIWLNAWGGE
                     WRTELAFGHSPGAHSEWYQPLGADRPLFMALSARIEQHPLRIYLPGGEQLGSLNVRRQ
                     AVQAELGWSLGASGEARLGVFRGSERAEREVGDPAQFESGRAAIAGLTASLTLDQFDR
                     PKLPREGYLLHADMTRAFSVLGTEQPYTHLNSRLDWAQTLGADTLRATLKYARTFARE
                     RIAGDQHGLGGFLNVSGMGPNQLLGQETLFARLMGYRQIAALPSAIGGGLYAGVSLEA
                     GRVRHALPGLYARQGWVPAASAYLVADSLLGPLFLGVGKATQSGLMGYLYLGSDF"
     gene            complement(3588..4334)
                     /locus_tag="PKN55_10850"
     CDS             complement(3588..4334)
                     /locus_tag="PKN55_10850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007341279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HNX71784.1"
                     /translation="MDFRWGMIAEYMPLFIQGMQMTLGITVIAVVLGTLIGLLMGMAR
                     LAEAKYGWQRYAVRFLVRLPATAYVTFFRGTPLFVQILLIHFALMPALIHPSDGLLLS
                     GDAAREIRQNYGAFLSGLVALTLNAGAYITEIFRAGIQSIDRGQTEAARSLGMSYGQT
                     MKFVIVPQAFRRMLPPLGNEAIMLLKDSSLVSAIGLAELAFAARTVAGVYSRYWEPYL
                     TISLVYLVLTLAMAAGVNWLEKRYQIAHHR"
     gene            complement(4655..>4941)
                     /locus_tag="PKN55_10855"
     CDS             complement(4655..>4941)
                     /locus_tag="PKN55_10855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012697695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HNX71785.1"
                     /translation="KRFESTPLALKELESGGVQAVVADNGVVINYLKNNASNGIATVA
                     DNDSFAPEFYGIAVKKGNAELQGKLNEGIKAIKADGTYDKIYAKYFGEKK"
BASE COUNT          831 a         1566 c         1643 g          901 t
ORIGIN      
        1 ccagcggcga tggctttgtg ttcctgctca cccgcgacga actgctggcg gcgcaggccc
       61 aagcttgacg tcgttcacca agccctgcgg gccttgttgt ggtgcgcatg gccaggcgcg
      121 ctgcgctaag cgtcgctcac caaacccagc gcagctgtcc tggcgaggtg tgcagtcgca
      181 gacagtacaa ggccgcgcaa caacgccagg ggattttgtg agcgactcgc gtcgtgctcg
      241 tacatcattc ggccgcccag tgtcacgctg gcgcggccgt gattttttgt cccctgaacg
      301 gagcccctgt ccttgagcgc cacccattct ccacgcgcct ggctggcgct gcattttact
      361 gttttgctgt ttggctgcgc cgggctgttt ggccacgccc tgccggtgtc gccctgggtg
      421 ttggtgtttg gccgcacgct gtttgctgcg ctaagcctgg cgctgtgcct gctggccttg
      481 cgccggccgt tgcgcccccg tacgggggag ggcggcaggc tggcgctggc ggcgggcgct
      541 tgcctggcct tgcactgggt gacgtttttt caggcgattc aggttggcgg cgtggcggca
      601 ggtttgctgg gctttgctgc ttttccggcg gcggtggtgg cgctggatag cctggggcaa
      661 cggcgctggc cagcctggcg cgatgtggcg atggtgggct tggtcagtgt gggcctggtg
      721 ctggtcacgc ccttgtccgc cgagggcgcc gccgtgcgtg agggcgtgtt gtggggtgtg
      781 ctgtcggcgc tactgtttgc cgtgctggcg ctgctgaacc gacgcagcgc cagcggtgac
      841 cctttgcgcc tggctggtct acaaaacgcc gccgccgcgc tgtggctgct gccgctggcg
      901 gtgggccagt tgccggcgct cagcctgcgc gattggctgg ggctggcggt gttgggcgtg
      961 gtattcactg cgctggcgca tgcgctgttt ttattcagcc tgcgggtgtt gccggcacaa
     1021 cgggtggcgg tggtcactgc gctggagccg gtatacggca tggcgtttgt ctggctgctg
     1081 tttgcccagg tgccggatgc acgcatgtgg ctgggcgggg cagtgattgt gctggccacc
     1141 ttgtgggctt cactgggtaa agcatccaag cactgaagct tttccagaaa aaccgcattt
     1201 agcgtggctc acaaaaccct cctggcgttg tgacacgacc ttgccgtact acctgtactg
     1261 tctgcggtcg tgcgcctagc caggaacgct gtgctgagtt ttgttagcca ctcctagcac
     1321 cgctcacaaa accctgctgg cgttgccgca cggctttgcc ggccggctaa aaatccgacc
     1381 ccagatacaa atagcccatc aaccccgatt gcgttgcctt gcccacgccc agaaacagcg
     1441 gccccagcag gctgtcggcc accagatagg ccgatgccgc cggcacccag ccctgacggg
     1501 catataaacc aggcagcgca tggcgcacgc ggccggcttc cagcgacacg ccggcataca
     1561 gcccaccacc gatggcgctg ggcaaggcgg caatttgccg atagcccatc agccgggcaa
     1621 acagggtttc ttgccccaat aactgattgg gccccatgcc agatacattc agaaaaccgc
     1681 ccaggccgtg ctggtcgccg gcgatgcgtt cacgggcaaa ggtgcgcgcg tatttcagcg
     1741 tggcgcgcag agtgtcggcg cccagcgttt gcgcccaatc cagccggctg ttcaggtggg
     1801 tgtagggctg ttcggtgccc aacacgctga aggcgcgggt catgtcagcg tgcaataaat
     1861 acccctcgcg cggcagcttg gggcggtcga attggtccag cgtcaggctg gcagtcagcc
     1921 cggcaatggc ggcgcggccg gattcaaact gcgccgggtc acccacttcg cgctcggcgc
     1981 gctcgctgcc gcgaaacacg ccaagccgcg cttcgccgct ggcccccagc gaccagccca
     2041 gctcggcttg cacggcttgc cggcgcacgt tcaggctgcc cagttgctcg ccgcccggca
     2101 gatagatgcg cagcgggtgt tgctcgatgc gcgctgacag cgccataaac agcggtcggt
     2161 cggcgcccag cggttgatac cattcgctat gcgcgccggg gctgtggcca aaagccagct
     2221 cggtgcgcca ctcgccgccc caggcattca gccagatgcg gtgatggctg aacagcagat
     2281 taaaagcatt ctggccatcg gtgcccgaag tcagccccag gcccatgcgc agataattgg
     2341 gcgccacgct gcgctcttgc ggcatcagct ccagcgtggt gcggttatct tcacgccaca
     2401 gccggtaacc cacgcgctcg aagtcgccgc tggcgtaggc ttggtcaagc tgccggctca
     2461 agtgggcgct gtccaggggc tggccggtgg cgggtttgag cgcagccaaa ctggcggccg
     2521 gggccatgtc gctgccggcg gcaatggtca ccgtgtcgat atgcggcgct gtcgggcgaa
     2581 gcgccagccg ggtggactgc cagcgggcat aatcggcttc gctgacgctg tagcggctga
     2641 gcgcctgcac atgcggctgc accgccagcc ggcccagttc ggccagttca tggtgggctt
     2701 tgaagtcagc cgcggtgtac tggccgagca tcggccggat cagcacgtcg cggttaaggt
     2761 cgagtttttg ttcttgctgg cgcacatttt gcagcacgcc aatctgggtg tattgcgcga
     2821 gcacatcgac aaacgagcgg atgtcttcgc gcttgagcga aggcgtgccg acatcaatgg
     2881 caataatcac atcggcgcag ccgttggggc cctgcaggtt ttccaccggc agctggcgcg
     2941 ccatgccgcc atccaccaat aagcggccgt ccagctctac cggctcgaac aggccgggca
     3001 ccgccatgct ggcgcgctgg gcgatggcca ggtcgccgtg gtcaaacaca taggcgtcgc
     3061 cggtaagcag gtcggtggcg accgcctgaa acggaatcgg caactgcgca aagctgtcca
     3121 ccagcacatt gccgcccagc tcacgcatca attggtcgat atgctgtgaa ctgaccgccg
     3181 cgcgcggcag acgcaagctg ccgtcacgaa agccggtgag caaatcaaaa taattgcggt
     3241 aatcgtcgcg cttgcgcttg aacggcaggt cggcgcgatt aagctggccg ctgagcacgc
     3301 ggtcccagtc ggtgcgctca aaggtggcgc ggatatgcgc cagcgactgg ccgctggcgt
     3361 aaaaaccgcc gatcagcgca ccggcgctgg tgccggccat gcaggcaatc gggatgcgca
     3421 ggcgctccag ctcggccagt acgcccaggt gggcaaaacc acgcgcgccg ccgccgccca
     3481 acaccacgcc cacgcgcggc ggggcgtcgg cggcaggcgc cgcgcctggg gccagcaggc
     3541 ccaaggcaat cagcgcgctg agcagcagac ggcgtatcac gacgcaatca acggtgatgg
     3601 gcgatctggt agcgtttttc cagccagttc accccggcgg ccatggccag ggtcagcacc
     3661 aggtacacca gcgaaatggt caggtagggc tcccagtagc gcgagtagac gccggccacg
     3721 gtgcgagcgg caaaggccag ctcggccagg ccgatggccg acaccagcga gctgtctttc
     3781 agcagcatga tggcttcgtt gcccagtggc ggcagcatgc ggcggaaggc ctgcggcaca
     3841 atgacgaact tcatggtctg gccgtagctc atgcccagcg agcgggcggc ttcggtctgg
     3901 ccacggtcga tggattgaat cccggcgcgg aagatttcgg tgatataggc gccagcgttc
     3961 aaggtgagcg ccaccaggcc agacagaaag gcgccgtagt tctggcggat ttcacgcgcg
     4021 gcatcgccgc tgagcaagag gccgtcactc gggtggatca gcgccggcat cagggcaaag
     4081 tgaatcagca gaatctgcac aaacagcggg gtgccgcgaa aaaaggtcac gtaggccgtg
     4141 gccggcaggc gcaccagaaa gcgcacggcg tagcgctgcc agccatattt ggcttcggcc
     4201 agccgggcca tgcccatcaa caggccgatc agcgtgccca gcaccacggc aatcaccgtg
     4261 atgcccagcg tcatttgcat gccctgaata aacagcggca tgtactcggc aatcatgccc
     4321 caacggaaat ccatacatcc tcgatatgca agatcggcag cgccgtccgg ccggatgggc
     4381 cgggcgcgcg ccagaccgct gcggatggca agcggcaggc gcgggcgcgc cggagtcccc
     4441 acgcggcgca aagggcgcgg ggccgggtgg atacacggcg cagggcgaac gagtgttcgt
     4501 ccgccctgcg cgccgcatgg gccgatgccg gcataagcac gacgccggcc caaaacttgc
     4561 gccgcccacc tccggcaagc cggggtgggc ggcgtgcggc gcatctgacc tatgatcggg
     4621 atgcgccatt caagggctgg attttacccg acgattactt tttctcgcca aaatacttgg
     4681 cgtagatctt gtcgtaagtg ccgtcagcct tgatggcttt gataccttcg ttcagcttgc
     4741 cttgcagctc ggcattgccc ttcttcacgg cgatgccata gaactccggt gcaaagctgt
     4801 cgttgtcggc cacggtggca atgccattgc tggcgttgtt tttcaggtag ttgatcacca
     4861 cgccattatc cgccaccaca gcctgcacgc cgccgctttc cagctctttg agcgccagcg
     4921 gggtggattc aaagcgcttg a
//
LOCUS       DAOPYX010000222         4924 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_21779_length_4924_cov_8.143767, whole genome shotgun sequence.
ACCESSION   DAOPYX010000222 DAOPYX010000000
VERSION     DAOPYX010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 4924)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4924)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4924
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21779_length_4924_cov_8.143767"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2454)
                     /locus_tag="PKN55_10860"
     CDS             complement(<1..2454)
                     /locus_tag="PKN55_10860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018150122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HNX71786.1"
                     /translation="MYSTSDAPAPDSVYANFKTIRRNGSVVAFEPGKISVAMSKAFLA
                     VAGSQGATSARIRDQVAHLTEQVVSALMRRKPEGGAIHIEEIQDQVELALMRSGEHDV
                     ARAYVLYREERSRQRALRGEADVAHVINVVKANGERQLLDVAALKALIKSACAGLEAD
                     TDAEHILSETLKNLYDGVAEDEVNKSAIMAARAMIEKDPSYNLVTSRLLLNSIRLEIL
                     GEAVSQEDMAERYAEYFPGYIKRGVAAELLDEKLGEFDLERLGRALVASRDTQFGYLG
                     LQTLYDRYFLHIDSQRIELPQAFYMRVAMGLALNEIDREARAIEFYNVLSTFDFMSST
                     PTLFNAGTRRSQLSSCYLTTIADDLDGIYEGIKENALLSKFAGGLGNDWTPVRAMGSH
                     IKGTNGKSQGVVPFLKVVNDTAVAVNQGGKRKGAVCAYLETWHADIEEFLELRKNTGD
                     DRRRTHDMNTANWIPDLFMKRVMEGGDWTLFSPSEVPDLHDLYGRAFEEAYVRYEAQA
                     ERGELRVAKKLPALSLWRKILTMLFETGHPWITFKDPCNIRSPQQHVGVVHSSNLCTE
                     ITLNTNDNEIAVCNLGSVNLVNHLHNGQLDQNKLARTVRTAMRMLDNVIDINFYPVKK
                     ARNANLKHRPVGMGVMGFQDCLHTLRIPYASPAAVEFADRSMEAVCYQAYWASTELAE
                     ERGRYPSYKGSLWDRGILPQDSIDLLAAERGGYLDVDRSETLDWAALRSRIASYGMRN
                     SNCVAIAPTATIANIIGVSASIEPTYQNLFVKSNLSGEFTVVNDYLVRDLKTAGLWDE
                     VMIADLKYFDGNVSRIDRIP"
     gene            complement(3016..4023)
                     /locus_tag="PKN55_10865"
     CDS             complement(3016..4023)
                     /locus_tag="PKN55_10865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014088567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HNX71787.1"
                     /translation="MFAWIAGRAGLLIALLLGGVALCLAPVPWLAAHGVSALMLAMLL
                     GMALAGVRRPGWQPGLNLAKGPLLRLGVALFGLRLTPADIAGLGVRVWVIDAAVLVST
                     FALAQWLGRRLRLDAPTRTLIGAGSAICGAAAVLATQPVVRADAQRAGVAVAGVTLFG
                     AAAMVLYPWLYHCLAPLGLSERAYGVLAGSTLHEVAHVVAAGQAVGAEAAASAVVAKM
                     LRVLLLAPFLLWLAHAWRRSGQLSADGAPTPRPPRPWFVLGFVAAAALAGSGWLPEAL
                     RQLLWQVDAWLLAAAMAALGLDIRPAMLRAAGWRPLALAGALWLWLMLGGGGINWLLS
                     R"
     gene            4161..>4924
                     /locus_tag="PKN55_10870"
     CDS             4161..>4924
                     /locus_tag="PKN55_10870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014088566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HNX71788.1"
                     /translation="MPLRLTLRELEVFVAIAEQHSVTRAARAVALSQSAASQALAQLE
                     SAVGVSLFDRVGRGLQLNAHGRAALPRARALLDDAQALQDALLGGALSLRLGASTTIA
                     NYLLPQRLAALRRQQPDCRLSLHVANTRDIVAAVLACTVDFGLVEGPCQHPELEVIPW
                     LDDELTLIAAPDHPLAHRPLSAAELADAPWLLREPGSGTREAVERALLPSLGRLRVEM
                     ELGDSEAIKRAVAAGLGVSCLSRRVAAELLTSGALV"
BASE COUNT          854 a         1611 c         1597 g          862 t
ORIGIN      
        1 cggaatgcgg tcgatgcggc tgacgttgcc gtcaaagtat ttcaggtcgg caatcatcac
       61 ttcgtcccac aggccggcgg ttttcaggtc gcgcaccagg tagtcgttga ccacggtgaa
      121 ctcgccggac aggttggact tcacaaacag gttctggtag gtgggctcaa tgctggccga
      181 cacgccaatg atgttggcga tggtggcggt gggggcaatg gccacgcagt tggagttgcg
      241 catgccgtag ctggcaatgc ggctgcgcag cgcggcccag tccagggttt ccgagcggtc
      301 cacatccaga tagccgccgc gctcggcggc cagcaggtcg atgctgtctt gcggcaggat
      361 gccgcgatcc cacaggctgc ccttgtacga cgggtagcgg ccgcgttctt cggccagctc
      421 ggtggacgcc cagtaagcct gatagcacac ggcttccatc gaacggtcgg caaattccac
      481 ggcggccggc gaggcgtagg ggatgcgcag ggtgtgcagg caatcctgga agcccatcac
      541 gcccatgccc accgggcggt gtttcaggtt ggcgttgcgg gcttttttca ccgggtagaa
      601 gttgatgtcg atgacgttgt ccagcatgcg catggcggtg cgcacggtgc gggccagttt
      661 gttctggtcc agctggccgt tgtgcaggtg gttcaccagg ttcaccgagc ccagattgca
      721 cacggcaatc tcgttgtcgt tggtgttcag ggtgatttcc gtgcacaggt tggagctgtg
      781 caccacgccg acgtgttgct gcgggctgcg gatattgcac gggtccttga aggtgatcca
      841 cgggtggccg gtttcaaaca gcatggtgag gatcttgcgc cacaggctga gtgccgggag
      901 ctttttggcc acgcgcagct cgccgcgctc ggcctgggct tcgtagcgca cataggcttc
      961 ttcaaaggcg cggccgtaca ggtcgtgcag gtccggcact tcggacggcg agaacagcgt
     1021 ccagtcgccg ccttccatca cccgcttcat gaacaggtca ggaatccagt tggcggtgtt
     1081 catgtcgtgc gtgcggcggc ggtcgtcgcc ggtgttcttg cgcagctcga ggaattcttc
     1141 gatgtcggcg tgccaggttt ccaggtaggc gcacactgcg cccttgcgct tgccgccttg
     1201 attgactgct accgccgtgt cgttcactac cttcaggaag ggcaccacgc cttgcgattt
     1261 gccattggtg cctttgatgt ggctgcccat ggcgcgcacc ggggtccagt cattgcccag
     1321 gccgccggca aatttggaca gcagggcgtt ttccttgatg ccttcgtaaa tgccgtccag
     1381 gtcatcggcg atggtggtca ggtagcacga cgacagctgg ctgcggcggg tgccggcgtt
     1441 gaacagcgtc ggggtggacg acatgaaatc aaaagtggac agcacattgt aaaactcaat
     1501 cgcgcgcgct tcgcggtcga tttcgttcag cgccaggccc atggccacgc gcatgtaaaa
     1561 cgcctgcggc agctcgatgc gctggctgtc gatgtgcaaa aagtagcggt cgtacagggt
     1621 ttgcaggccc aggtagccaa actgggtgtc gcggctggcc accagcgcgc ggcccaggcg
     1681 ctccaggtca aattcgccca gtttttcgtc cagcaactca gcggcaacgc cgcgcttgat
     1741 atagccgggg aagtattcgg cgtaacgctc ggccatgtct tcctggctga cagcttcgcc
     1801 gaggatttcc aggcggatgc tgttgagcag caggcgcgag gtgaccagat tgtaagacgg
     1861 gtctttttcg atcatggcgc gggcggccat gatggcggat ttgttgactt cgtcttcggc
     1921 cacgccgtcg tacaggtttt tcagggtttc cgacaggatg tgttcggcgt cggtgtcggc
     1981 ttccaggccg gcgcaggcgg atttgatcag cgccttgagc gccgccacgt ccagcagctg
     2041 gcgctcgccg ttggctttca ccacattgat cacatgggcg acgtcggctt cgccgcgcag
     2101 ggcgcgctgg cgcgagcgtt cttcccggta cagcacatag gcgcgggcca catcgtgttc
     2161 gcccgagcgc atcagcgcca gttccacttg atcctggatt tcttcgatgt ggatggcgcc
     2221 gccttccggc ttgcggcgca tcagcgcgct gaccacttgt tcggtcaggt gcgccacctg
     2281 gtcgcggatg cgcgccgacg tggcgccctg gctgccggcc acggccaaaa aggccttgct
     2341 catggcgaca gagattttgc ccggctcaaa agccaccacg ctgccattgc ggcggatggt
     2401 cttgaagttg gcgtaaacag agtcaggggc cggcgcgtcg ctggtgctgt acatgggtgg
     2461 agaattcctt cgtgtgattc atcaatggca cagcctgccg cccacctgtg gataaccttg
     2521 tggaagacat gtgtgcacct tgtgtgtaac cacaagatat agttgttgcg agggttggat
     2581 agccacaaat gctagtggtt agtcgggcgt tcgacaagaa ttgacagccg gcaaaaaatg
     2641 cggttttgcc ccacgctggc aagcatggcc ggacggcaaa tccagcactg gcaaggactt
     2701 gcgtgcacct gctatgcgcc cggtataaaa tgcgtccgat gcagaatgtc gcaatcctga
     2761 aacacaagat gtggttttct tgcgacccga cagacacaag ccactgttgg cgccaagcgg
     2821 ccggtgatga atcgaccgtc caggttgggt cagtgcagcg ggctgtggat cacctcgtgt
     2881 actttaccgg cctgcggatg gggtgagcgt tactctccac accctcctgg tgttgttcgg
     2941 tttggtcata tttcttgttc agcctgccgc cgcgcgtctg gctggtggca cagtgctcag
     3001 tgtggtaagt cgcgcttagc ggctcaacag ccagttaatc ccgccgccgc ccagcatcaa
     3061 ccacagccac agcgcgccgg ccagcgccag cggccgccag ccggcggcgc gcagcatggc
     3121 cgggcgaata tccaggccca gcgcggccat ggcggcggcc agcagccagg cgtccacctg
     3181 ccataacagc tggcgcagcg cctcgggcag ccagccgctg ccggccagcg cggcggcggc
     3241 gacaaaaccc agcacaaacc acggccgggg cgggcgcggc gttggcgcgc catcggcgct
     3301 cagctgcccg ctgcgacgcc aggcgtgcgc cagccacagc aaaaacggcg caagcaacag
     3361 cacccgcagc attttggcca ccaccgcgct ggcggcggcc tcggcgccca ccgcctggcc
     3421 ggccgccacc acatgcgcca cttcgtgcaa ggtggagcca gccagcacgc cataggcgcg
     3481 ctcgctcagg cccagcggcg ccaagcagtg atacagccag gggtagagca ccatggccgc
     3541 cgcgccaaac agcgtcaccc cggccaccgc cacgccagcg cgctgggcgt cggcgcgcac
     3601 caccggctgg gtggccaaca ccgctgccgc gccgcaaatg gcgctgcccg cgccaatcag
     3661 cgtgcgggtg ggcgcatcca gccgtaggcg gcggcccagc cattgcgcca gggcaaaggt
     3721 gctgaccaac accgccgcgt cgatcaccca cacccgcacg cccagcccgg caatgtccgc
     3781 cggggtcagc cgcaaaccaa acagcgccac ccccagccgc aacagtgggc ccttggccag
     3841 gttcaggcca ggctgccagc cagggcggcg cacgccggcc agcgccatgc ccagcaacat
     3901 cgccagcatc aaggccgaca ccccatgcgc ggccagccac ggcaccggcg ccaagcacag
     3961 tgccacgccc cccagcagca gggcaatcag caagccggcg cgaccggcaa tccaggcaaa
     4021 catggcgaca tcctcgcaaa cagtgacagc catgcagcct acgcgggcgc agtcaattga
     4081 taaaacggat aatatagata gaattaattg gtataactga tcaatggcgg cgccacgccg
     4141 cgcccaccat ggagccccac atgcccctgc gcctgaccct gcgcgaactg gaagtctttg
     4201 tcgccatcgc cgaacaacac tccgtcaccc gcgccgcccg cgcggtggcg ctgtcgcaat
     4261 cggcggccag ccaggcgctg gcccagctgg aaagcgcggt gggggtgagt ttgtttgacc
     4321 gggtggggcg cggcttgcag ctgaacgccc acggccgcgc cgccttgccg cgcgcccgcg
     4381 ccctgctgga cgacgcgcaa gccctgcaag acgcgctgct gggcggcgcg ctgtcgctgc
     4441 ggctgggcgc cagcaccacc atcgccaatt acctgctgcc gcaacgcctg gcggcgctgc
     4501 gccgccagca accggattgc cggctgagcc tgcacgtggc caacacccgc gacatcgtcg
     4561 ccgccgtgct ggcctgcacc gtggattttg gcctggtgga agggccatgc cagcacccgg
     4621 aactggaagt catcccctgg ctggacgatg aactgacgct gatcgccgcc cccgaccacc
     4681 cactggccca ccgcccgctc agcgccgccg aactggccga cgccccctgg ctgctgcgcg
     4741 aacccggctc cggcacccgc gaagcggtgg agcgcgccct gctgcccagc ctgggccgct
     4801 tgcgagtgga aatggagctg ggcgactccg aagccatcaa acgcgccgtc gccgccggcc
     4861 tgggcgtcag ctgcctgtca cgccgcgtcg ccgccgaact gctgaccagc ggcgcgctgg
     4921 tgga
//
LOCUS       DAOPYX010000223         4922 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_21792_length_4922_cov_10.510171, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000223 DAOPYX010000000
VERSION     DAOPYX010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 4922)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4922)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4922
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21792_length_4922_cov_10.510171"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..356
                     /gene="rplI"
                     /locus_tag="PKN55_10875"
     CDS             <1..356
                     /gene="rplI"
                     /locus_tag="PKN55_10875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003772198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HNX71789.1"
                     /translation="QGKAKRATEANLKEFEARRAELEKQQAEILVAAQARGEKLEGAV
                     VVVAQKAGVDGRLFGSVGNADIADAITATGVEVKKAEVRLPEGPFKAIGEYSVEVALH
                     HDVVVTITLQVTGAA"
     gene            479..1468
                     /gene="mltG"
                     /locus_tag="PKN55_10880"
     CDS             479..1468
                     /gene="mltG"
                     /locus_tag="PKN55_10880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018748905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HNX71790.1"
                     /translation="MLKRILVVLLLLLGLSAGGAWMALSPYQPANLPVEFTIQPNQGL
                     SDIAHTLEDSGAIRHAGLFMLWAKLSGNAGRLKAGDYRLNRAMSMPGVMDKLVRGETS
                     LQSVIIREGWTLRQFRAELDRHPALRHDTRDWSEAELLAKLKLDARHGEGLFFPDTYL
                     FLRGSSDILVLRQAAQNMRRELERAWAERGPDLPYASPYEALIMASLIEKETGQAADR
                     PMIARVFINRLKLGMRLQTDPSVIYGLGEAFDGNLTRAHLQTDTPYNTYTRAGLPPSP
                     IALPGRAALHAALHPADGGALYFVARGDGSSQFSLTLEEHNRAVRRYILGKKD"
     gene            1474..2106
                     /gene="tmk"
                     /locus_tag="PKN55_10885"
     CDS             1474..2106
                     /gene="tmk"
                     /locus_tag="PKN55_10885"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HNX71791.1"
                     /translation="MACFITLEGIDGAGKTSHLPAIAALLAEHGIDALLTREPGGTPL
                     GERLRALLLDPATQATLDTETLLMFAARCEHVQTVIRPALASGRWVVSDRFTDATFAF
                     QGGGRGVDSARIATLEDWVQQGLQPDLTLLFDAPTEVAHARMAGERQLDRFEQEAAAF
                     HQRVRAAYLARAAADPTRIVLIRADQPLAQVRADALAAVAALLAAQGARA"
     gene            2103..3095
                     /gene="holB"
                     /locus_tag="PKN55_10890"
     CDS             2103..3095
                     /gene="holB"
                     /locus_tag="PKN55_10890"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012698141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HNX71792.1"
                     /translation="MSLLPWQDADWTRFADQFARLPHALLFTGAAGIGKDVFARHVAQ
                     ALLCEHPRADQRPCGQCEACHWFAQDAHPDFRLLRPDHDEPAADGKPAARKLEVIKIE
                     AVRHTIDFAYLSSHRGGRRVVLIDPAESMNLNAANALLKVLEEPPADVVFLLVSDALQ
                     KLLPTLRSRCRIFPLTAPSQAQALAWLNAHGEADAAVELAHAGGAPFALDDAAWRPLR
                     RKLLSALAQPGMTAILDAAGALDSAKLPLAAPVGWMQRWLHDLLGLRLAGLIRYYPDE
                     RAALAALAPRLNVARLPAFEQSLLHASRFGHHTLNVRMQLEQLLLAYQGLFAAA"
     gene            3112..3888
                     /locus_tag="PKN55_10895"
     CDS             3112..3888
                     /locus_tag="PKN55_10895"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011137267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HNX71793.1"
                     /translation="MLVDSHCHLNFPDLASRMDDVRANMAANAVSHALLIGVNRPDYP
                     AVRALAEQYDNFYATVGVHPDVQDSPDFSEDELVAEAQHPKVVGIGETGLDYYWCKGD
                     LEWQRERFRRHIRAARRSGLPLVIHTRESAQDTLAVMREAGAEQAGGVMHCFTESWEV
                     AEGALELGFYISFSGIVTFKNATALKEVARRVPLERLLVETDSPYLAPMPHRGKTNEP
                     AYVRHVAAHVAELRGMALEDLAAASTDNFFTLFAKASRGH"
     gene            3878..4651
                     /locus_tag="PKN55_10900"
     CDS             3878..4651
                     /locus_tag="PKN55_10900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012698139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNX71794.1"
                     /translation="MDIELTILGCGSSSGTPAIGCDCPTCASPDPKNRRTRASALLRA
                     NGVNFLIDTGPDLRQQALRENLRQVDAVLYTHPHADHLNGIDDLRAFCYLKRGAIPLF
                     GSPFMMGNIRERFGYALHAAGAQWDKPVLETHAVEGPFSFSGVTVTPIPVMHGPWPIL
                     GWRIGDVAYLTDVSAIPDASWPLLAGVRLLLLDCLRRTPYPSHLSVTQALALAAQLGA
                     AHTVLIHMTHELEYHSLSADCPAGVEVGYDGMRLQLSYG"
BASE COUNT          857 a         1724 c         1509 g          832 t
ORIGIN      
        1 cgcaaggcaa ggccaagcgc gccaccgaag ccaacctgaa ggaatttgaa gcccgccgcg
       61 ccgagctgga aaagcaacag gccgaaatcc tggttgctgc ccaagcccgt ggcgaaaagc
      121 tggaaggcgc cgttgttgtt gtcgcccaga aggctggcgt ggacggccgt ctgttcggct
      181 cggtcggcaa cgccgacatc gctgacgcca tcaccgccac cggcgtggaa gtgaaaaagg
      241 ctgaagtgcg cctgccggaa ggcccgttca aggccatcgg cgaatactcg gtggaagtcg
      301 ccctgcacca cgacgtggtg gtgaccatca ccctgcaagt gaccggcgca gcttaattcg
      361 cgacgcttca caaaaaaacc gccagcggat tccgccggcg gtttttttat gcctgttcct
      421 gttctcttcg ccatccccct gccaaaatag cagcctgtct gttgcgcgct gaaccgctat
      481 gctgaaacga attcttgtag tgctgttgct cttgctgggc ctgagcgccg gcggcgcctg
      541 gatggcgcta agcccatacc aaccggccaa tctgccggtg gaattcacca tccagcccaa
      601 tcagggctta agcgacattg cccacaccct ggaagacagc ggcgccatcc gccatgccgg
      661 gctgttcatg ctgtgggcca agctcagcgg caacgctggc cgattaaaag ccggcgatta
      721 tcggctgaac cgcgccatga gcatgccggg ggtgatggac aaattggtgc gcggcgaaac
      781 cagcctgcaa tcggtgatca tccgcgaagg ctggaccttg cgccagttcc gcgccgaact
      841 tgaccgccac ccggcgctgc gccacgacac ccgcgactgg agcgaagccg agctgctggc
      901 caaactcaag ctggacgccc gccacggcga aggcttgttt tttcccgaca cctatctgtt
      961 tttgcgcggc agcagcgata ttctggtgct gcgccaggcg gcgcaaaaca tgcgccgcga
     1021 gctggagcgc gcctgggccg aacgcggccc cgacttgccc tatgcctcgc cgtacgaagc
     1081 gctgatcatg gccagcctga tcgaaaaaga aaccggccag gccgccgacc ggccgatgat
     1141 cgcccgggtg ttcatcaacc gcctgaagct gggcatgcgc ctgcaaaccg accccagcgt
     1201 gatctacggc ctgggcgaag catttgacgg caacctcacc cgcgctcatc tgcaaaccga
     1261 caccccatac aacacctaca cccgcgccgg cctgccgccc tcgccgattg ccctgcccgg
     1321 ccgcgcggcg ctgcacgctg ccctgcaccc ggccgatggc ggcgcgctgt attttgtcgc
     1381 ccgaggcgat ggcagctcgc aattctccct caccctggaa gaacacaacc gcgcagtgcg
     1441 ccgctatatt ctgggcaaga aagactgaac gacatggcct gtttcattac ccttgaaggc
     1501 atcgacggcg ccggcaaaac ctcgcacctg ccggcgattg ccgccctgct ggccgaacac
     1561 ggcatcgacg ccctgctcac ccgcgaaccc ggcggcacgc cgctgggcga acgcctgcgc
     1621 gccctgctgc tggatccggc cacccaggcc accctggaca ccgaaaccct gctgatgttc
     1681 gccgcccgct gcgaacatgt gcaaaccgtc atccgcccgg cgctggccag cggccgctgg
     1741 gtggtgtcgg accgctttac cgacgccacc tttgcttttc agggcggcgg gcgcggggtg
     1801 gacagcgcac gcatcgccac gctggaagac tgggtgcagc aaggcttgca acccgacctc
     1861 accctgctgt tcgacgcccc caccgaagtg gcgcacgcgc gcatggccgg cgaacgccag
     1921 ctggaccgct ttgaacaaga agccgccgct tttcaccagc gcgtgcgcgc cgcctacctg
     1981 gcgcgcgccg ccgccgaccc cacgcgcatc gtgctgatcc gcgccgacca gccgctggcg
     2041 caggtgcgcg ccgacgcgct ggcggcggtg gctgcgctgc tggcggcgca aggggcgcgc
     2101 gcatgagcct gctgccctgg caagacgccg actggacgcg ctttgccgat caatttgccc
     2161 gcctgccaca cgctttgctg tttaccggcg ccgccggcat cggcaaggac gtgtttgccc
     2221 gccatgtggc gcaggcgctg ctgtgcgaac acccgcgcgc cgaccaacgc ccctgcggcc
     2281 agtgcgaagc ctgccactgg tttgcccagg acgcccaccc ggattttcgc ctgctgcgcc
     2341 ccgaccacga cgagcccgcc gccgacggca aacccgccgc gcgcaagctg gaagtgatca
     2401 aaatcgaagc ggtgcgccac accatcgact ttgcctacct cagctcgcat cgcggcggcc
     2461 ggcgggtggt gctgatcgac ccggcggaaa gcatgaacct caacgccgcc aacgccctgc
     2521 tgaaagtgct ggaagaaccg ccggccgatg tggtgttttt gctggtcagc gacgcgctgc
     2581 aaaaactgct gcccacgctg cgcagccgct gccggatttt tccgctcacc gcgcccagcc
     2641 aggcgcaggc gctggcctgg ctgaacgccc acggcgaagc cgacgccgcc gtcgagctgg
     2701 cgcacgctgg cggcgcgccg tttgcgctgg acgacgccgc ctggcggccc ctgcggcgca
     2761 agctgctgag cgcgctggcg cagccgggca tgacggcgat tctggacgcc gctggcgcgc
     2821 tggacagcgc caagctgccg ctggccgcgc cagtgggctg gatgcagcgc tggctgcacg
     2881 atttgcttgg attgaggctg gccgggctga tccggtatta tcctgatgaa cgcgcggcgc
     2941 tggccgcgct ggcgccgcgc ctgaatgtcg ccaggctgcc ggccttcgag cagtccttgt
     3001 tgcacgcctc gcgctttggc caccacaccc ttaacgtgcg catgcagctg gaacagctgc
     3061 tgctggccta ccaaggcctg tttgccgccg cctgaccttc acgagccccc tatgctggtt
     3121 gactctcact gccatctgaa cttccccgac ctggccagcc gcatggacga cgtgcgcgcc
     3181 aatatggccg ccaacgccgt cagccacgcc ttgctgattg gggtaaaccg cccggattac
     3241 ccggcggtgc gcgcactggc tgagcaatac gacaattttt acgccaccgt cggcgtgcac
     3301 cccgatgtgc aggacagccc ggacttcagc gaagacgaac tggtggccga agcccagcac
     3361 ccgaaagtgg tcggcattgg cgaaaccggg ctggactact actggtgcaa gggcgatctg
     3421 gaatggcagc gcgagcgctt tcgccgccat atccgcgccg cccggcgcag cggcctgccg
     3481 ctggtgatcc acacccgcga atcggcgcaa gacaccctgg cggtgatgcg cgaagccggc
     3541 gccgagcagg ccggcggggt gatgcattgc tttaccgaaa gctgggaagt ggccgagggc
     3601 gcgctggagc tggggtttta tatttcgttt tctggcattg tcaccttcaa gaacgccacc
     3661 gcgctgaaag aagtcgcccg ccgcgtgccg ctggagcggc tgctggtgga aaccgactcg
     3721 ccgtatctgg cccccatgcc gcatcgcggc aagaccaacg aaccggccta tgtgcgccat
     3781 gtcgccgccc atgtggccga gctgcgcggc atggcgctgg aagacctggc cgccgccagc
     3841 accgacaact tttttaccct gttcgccaag gcgtcgcgtg gacattgagc tgaccattct
     3901 gggctgcggc tccagctcgg gcaccccggc cattggctgc gactgcccga cctgcgcctc
     3961 gcccgacccg aaaaaccggc gcacccgcgc cagcgcgctg ctgcgcgcca atggcgtcaa
     4021 tttcctgatc gacaccggcc cggatttgcg ccagcaagcg ctgcgcgaaa acctgcgtca
     4081 ggtggacgcc gtgctctaca cccacccgca cgccgaccac ctgaacggca tcgacgacct
     4141 gcgcgccttt tgctacctca agcgcggggc gattccgctg tttggcagcc cgttcatgat
     4201 gggcaatatc cgcgagcgct ttggctacgc gctgcacgcc gctggcgcgc agtgggacaa
     4261 accggtgctg gaaacccacg cagtggaagg cccgttcagc ttctctggcg tgacagtcac
     4321 gcccatcccg gtcatgcacg gcccctggcc gattctgggc tggcggattg gcgatgtggc
     4381 ctacctcacc gatgtctcgg cgattcccga cgccagctgg cccttattgg ccggcgtgcg
     4441 cctgctgctg ctggattgcc tgcgccgcac gccttacccc tcgcacctga gcgtgaccca
     4501 ggcgctggcg ctggccgcgc aactgggcgc cgcgcacacc gtgctgattc acatgaccca
     4561 cgagctggag taccacagcc tgagcgccga ctgcccggcc ggggtggaag tgggctatga
     4621 cggcatgcgc ttgcagctat cctacggctg ataaaaccct cctggctttg ttgcacgacc
     4681 ttgccgtact gcttgtacgg tctgcgctca cacgcctagc caagatcgct gcgcagggtt
     4741 ttgtaagcgg caatacagca caacccggac tgtttccgcc ttgcttgatt aggtatttct
     4801 acttaatctt agcacgggta catttgagac atcacttgcc caaacgccaa accatgcaga
     4861 ttgacttgtt ggtttgacga gacaatgcca caaggctatg ctggcgcgcg catacacaag
     4921 tc
//
LOCUS       DAOPYX010000224         4878 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_22063_length_4878_cov_7.429608, whole genome shotgun sequence.
ACCESSION   DAOPYX010000224 DAOPYX010000000
VERSION     DAOPYX010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 4878)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4878)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4878
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22063_length_4878_cov_7.429608"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1506)
                     /locus_tag="PKN55_10905"
     CDS             complement(<1..1506)
                     /locus_tag="PKN55_10905"
                     /inference="COORDINATES: protein motif:HMM:NF019974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNX71795.1"
                     /translation="MTHRLTLGQRLFLLVSLPLLGMVGYAALTAWAHWQEAVNMRRAA
                     ALTEVSTRSGDVIHLMQVERGASVTGDQALLAQARSALDGVIRTMRASVDSLDDLTSD
                     DRALALQELQALDTLATVRQGVDQHGLTSTQFIAQYTGLIEKLMNLATVGVQRATHAD
                     LTRQMTAQLALLCEKEFAGRERAQLAAALAQQSLDEAARRKVLEGVGRQQSCLTEFNA
                     LADPALRDAYVAVSATPAYQDAVNMREKILTLGPGQSGIDKDTWFAAATSRMEAIKGM
                     LDTFSHAMIAQADGLKDAASQRALLSAGVALVAILLVLTLSMLTMRALRREVGALQAV
                     MDNTSRQLDLTHRANLPGEDEIASIGRSFDQLVDAFARALGQVEHDARQLADAAGGLS
                     SVSRQAAAGSAEQTHASSQIAVSTEEISAGITQVVDSANASEACAGETRRQASAGRDS
                     MRQAAAEITEMADAVQQASGTIHQLHERSQAISQLIGSIQGIADQTNLLALN"
     gene            complement(1793..3025)
                     /locus_tag="PKN55_10910"
     CDS             complement(1793..3025)
                     /locus_tag="PKN55_10910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019959407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HNX71796.1"
                     /translation="MVLNVIWSAFILLALAAALAQAVWLGHTDIFATMVKALFDAAKS
                     GFEISLGLTGVMALWLGLMKIGERAGLVALAARALAPLFARLFPGVPRDHPALGSITL
                     NMAANMLGLDNAATPLGLKAMRELQTLNPSADTASDAQILFLVINTASITLFPVTIFA
                     YRAQMGAADPTDVFIPLLLTSYLGTLSALALTGAIQRLKLLDPVVLAYLAGLSALVLG
                     SAWWFAHLPAAQMQAQSSLLSNAVLLGMIGALLLAALYKRVNVYDSFIEGAKEGFQVA
                     VGIIPYLIAMLAAIALLRASGALDLLMGGLKQLVAAGGWDTRWVDALPTGVMKSLSGS
                     GARALMLDTLKTQGADSFAGRLVSILQGSSETTFYVLAVYFGAVNIRNTRYAAACGVF
                     ADVMGFVAAVWVAYLFFG"
     gene            3175..4170
                     /locus_tag="PKN55_10915"
     CDS             3175..4170
                     /locus_tag="PKN55_10915"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNX71797.1"
                     /translation="MTSLAHRVRLILTLLAALSLGLAACSPVALHAWWSESSARAQGF
                     APARLSLAHGGQLAYREAGHGPTLLLIHGFGGNGLLHWRRSMSELVEQYHVVTPDLLG
                     FGDSHAPGPLTLDAQVDALIALIEARRLGEVRVAGISYGGFVALELARRLPLSVRRVV
                     LINSPGPIYQAADLRALERRAQVRSVDELFVPRTPAELRRLIGMTRTQPVDASDWLLD
                     DMLARYYRGHETGMLELLHDLQASQARYSQRLLDARPASAVVWSEGDAVFPPPLGRLL
                     AEHLQAPMILVENAGHSLLADQPAMAVQALRLALSMPLDPSRTVRLGPTDAPMGP"
     gene            complement(4173..4874)
                     /gene="ubiG"
                     /locus_tag="PKN55_10920"
     CDS             complement(4173..4874)
                     /gene="ubiG"
                     /locus_tag="PKN55_10920"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011134586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008689 - 3-demethylubiquinone-9
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HNX71798.1"
                     /translation="MSNVDQAELEKFSQVAHKWWDTSSEFKPLHDINPLRLDYISQQA
                     GGLAGKRVLDVGCGGGILSEGLARAGAEVLGIDLAKKSLKVAQLHALESGVAVSYRCV
                     AVEDLAAEAPGSFDVVTCMEMLEHVPDPQSVVRACATLVKPGGAVCFSTLNRNLKSYA
                     LAVLGAEYVLNMLPRGTHEYARFLKPSELARMARHAGLDVSDICGMSYNPLTQVYRLG
                     EDSAVNYLMGCRRIG"
BASE COUNT          886 a         1608 c         1514 g          870 t
ORIGIN      
        1 attcagcgcc agcagattgg tctggtcggc aatgccctgg attgagccaa tcagctggct
       61 gatggcctgt gagcgctcgt gcaactggtg aatggtgccg gacgcctgct ggacggcatc
      121 ggccatttcg gtgatttcag ccgccgcctg gcgcatgctg tcgcgaccgg cgctggcctg
      181 gcggcgggtt tcgccggcac aggcttcgct ggcattggcg ctgtccacca cctgggtgat
      241 gccggcgctg atttcttcgg tggagacggc aatctggctg gaagcatggg tttgctcggc
      301 cgaaccggct gcggcctgac gcgacaccga cgacaggccg cccgcggcat cggccagttg
      361 ccgggcatcg tgttccacct ggcccagcgc acgggcaaag gcatccacca gttggtcaaa
      421 cgagcggccg atgctggcga tttcgtcttc gccaggcaga ttggcccggt gggtcagatc
      481 cagctggcgg ctggtgttgt ccatcacggc ctgcaaggca cccacttcac gacgcaaggc
      541 gcgcatggtg agcatggaca gtgtgagtac cagcagaatg gccaccaggg ctacgccggc
      601 agacaacagc gcgcgctggc tggcggcgtc cttcagtccg tcagcctggg caatcatggc
      661 gtgcgagaag gtatccagca tgcccttgat ggcttccatg cggctggtgg cggcagcaaa
      721 ccaggtgtcc ttgtcgatgc cggattgccc gggacccagc gtcaggattt tttcgcgcat
      781 gttcaccgca tcctgatagg ctggggtggc gctgaccgcc acataggcat cgcgcaaggc
      841 tggatcggcc agggcgttga attcggtcag acagctttgc tgacgcccca cgccctccag
      901 caccttgcga cgggctgctt cgtccaggct ttgctgggcc agcgccgcgg ccagttgggc
      961 gcgctcgcgg ccggcgaatt ctttttcgca cagcagcgcc agttgggcgg tcatctgtcg
     1021 ggtgaggtcg gcgtgggtgg ctcgctgcac acccacagtg gccagattca tcagtttttc
     1081 aatcagcccg gtgtactggg caatgaattg cgtggaggtc agaccatgct gatccacgcc
     1141 ttgacgcaca gtggccagcg tgtccagtgc ctgcaattcc tgaagggcca gggcgcggtc
     1201 gtcgctggtc agatcatcca gactgtccac gctggcgcgc atggtgcgga tcacaccatc
     1261 cagggcactg cgcgcctggg ccagcagagc ctgatcgccg gtgacgctgg cgccgcgctc
     1321 gacctgcatc agatgaatca cgtcaccgga acgggtggag acttcggtca gcgccgccgc
     1381 gcggcgcatg ttgacggctt cttgccagtg ggcccacgct gtcagcgcag cgtagcccac
     1441 catgccaagc agcggcagac tgaccagcag aaacagacgc tggcccaagg tcagacggtg
     1501 ggtcatgatg gtgttttctt ctcgttctga cgcggcgtgc gccgcgcacg gatgcggtgc
     1561 agccatactg acgatggctc ttcatgccag tcagtgccgg ggcagaacaa tgcagtgcaa
     1621 cattcgcgcc gacaccgcca gcagcagtga gggcagtata cccagttgta gccgctgcgc
     1681 cgggtaaatg cgcaaaacct tgacctaagt cggcatgggt gatttttttg tcataacgca
     1741 ccctggctgg catgacgcac agcagcatag tggcatgttt ctccaccgga aatcagccaa
     1801 aaaacagata ggccacccac accgccgcca caaaacccat cacatcggca aacaccccgc
     1861 aggcggcggc atagcgggtg ttgcggatgt tcaccgcgcc aaaatacacc gccagcacat
     1921 aaaacgtggt ttcgctgctg ccttgcaaaa tcgacaccag ccggccggca aacgaatccg
     1981 cgccctgggt tttcagcgtg tccagcatca acgcccgtgc accactgcct gacagcgatt
     2041 tcatcacccc ggtgggcagc gcatccaccc agcgcgtatc ccagccgccg gccgccacca
     2101 gctgcttcaa gccgcccatc agcaaatcca gcgcgcccga ggcgcgcagc agggcaatcg
     2161 ccgccagcat ggcaatcaga tagggaataa tccccaccgc cacctgaaaa ccctctttgg
     2221 cgccctcaat aaagctgtcg tagacattta cccgcttgta cagcgccgcc agcagcagcg
     2281 cgccaatcat gcccagcagt acggcattac tcagcaagga cgactgcgcc tgcatctgtg
     2341 cggctggcag gtgagcaaac caccaggcgc tgcccagcac cagcgcgctc aggccggcca
     2401 gataagccag caccaccggg tccaacaatt tcagccgctg aatggcgccg gtcagcgcca
     2461 gcgccgacag cgtgcccaga tagctggtga gcagcagcgg aataaacaca tcagtggggt
     2521 cggcggcgcc catttgcgcg cggtaggcaa agatagtcac cggaaacaaa gtaatcgacg
     2581 cggtgttgat caccaaaaac agaatctgcg catcgctggc ggtgtcggcg ctggggttga
     2641 gcgtttgcag ctcgcgcatg gccttcagcc ccagcggggt ggcggcgttg tccagcccaa
     2701 gcatattggc cgccatgttc agcgtgatgc tgcccagcgc cggatggtcg cgcggcacac
     2761 cgggaaacaa cctggcaaac aagggcgcca gcgcgcgcgc cgccagcgcc accaggccgg
     2821 cgcgctcgcc gattttcatc aaacccagcc acagcgccat cacgccggtc aggccgagtg
     2881 aaatttcaaa gccgcttttg gcggcgtcaa atagcgcctt gaccatggtg gcaaagatat
     2941 cggtgtgccc cagccacacc gcttgcgcca gcgcagcggc cagggcaagc aggataaacg
     3001 ccgaccagat gacattaagc accatgttga tcatttcctg tggatgagca ggaaacagcc
     3061 cgcccaccgt aaacttgcga attgttgaca atatctgcaa gcattatgat atcagcctga
     3121 accgtagcct gaagctggcg cggcccctgc ctgttttccg gacatcccct acccatgaca
     3181 tcccttgcgc accgcgtgcg cctgattctc accctgctgg ccgccctgtc gctgggcctg
     3241 gccgcctgct ctccggtggc gctgcacgcc tggtggagcg aatccagcgc ccgcgcccag
     3301 ggctttgctc cggcccgact cagtctggcc catggcggcc agctggctta tcgcgaagcc
     3361 ggtcacggcc ctacgctact gctgatccac ggctttggcg gcaatggcct tttgcactgg
     3421 cgacgcagca tgagcgaact ggtcgaacaa taccacgtgg tcactcctga cctgcttggc
     3481 tttggcgact cccacgcgcc cgggccactg accctggatg cccaggtcga tgcactgatt
     3541 gccctgatcg aagcgcgccg gctgggcgag gtgcgggtgg cggggatttc ctacggcggc
     3601 tttgtcgcgc tggagctggc ccggcgtctg ccattgagcg tgcgtcgggt ggtgttgatc
     3661 aactcgcccg gaccgattta ccaagcggcc gatctgcgcg cactggagcg gcgtgcccag
     3721 gtgcgttcgg tggacgagtt gtttgtgccg cgcaccccgg ccgagctgcg ccggctgatc
     3781 ggcatgaccc gcacccagcc ggtggatgcc agcgactggc tgctggacga tatgctggcg
     3841 cgttactacc gtggccacga aaccggcatg ctggaactgc tgcacgacct gcaagccagc
     3901 caggcacgct acagccagcg cctgctggat gcgcggccag ccagcgccgt ggtgtggagc
     3961 gaaggtgacg cggtgtttcc gccgcccttg ggccgtctgt tggccgagca cctgcaagcg
     4021 ccgatgattc tggtggaaaa cgccggtcac agcctgctgg ccgatcagcc ggccatggcc
     4081 gtacaggcgc tgcgcctggc gctgtcgatg ccgctggacc catcacgcac cgtgcgacta
     4141 gggccgaccg atgcgccgat ggggccgtag gctcagccga tgcgtcggca gcccatcaga
     4201 taattcactg cgctgtcctc gcccagtcga tacacctggg tgagcgggtt gtagctcatg
     4261 ccgcagatgt cgctgacatc cagcccggca tgacgcgcca tgcgcgccag ttcggacggc
     4321 ttgagaaagc gcgcgtattc atgcgtgccg cgcggcagca tgttcagcac gtattccgcc
     4381 cccagcaccg ccagcgcata gcttttcaga ttgcggttca gcgtggaaaa acacaccgcg
     4441 ccgcccggct tcaccagggt ggcgcaggcg cgcaccacgc tttgcgggtc gggcacgtgt
     4501 tcgagcattt ccatgcaggt caccacgtca aacgaccccg gcgcttcggc ggccaggtct
     4561 tccaccgcca cgcagcggta gctgaccgct acgccggatt ccagcgcgtg cagctgcgcc
     4621 accttcagcg actttttcgc caggtcgatg cccagcacct cggcgccggc gcgcgccagc
     4681 ccttcggaca aaatgccgcc gccgcagccc acgtccagca cgcgcttgcc ggccaggccg
     4741 ccggcctgct ggctgatata gtccagccgc agcgggttga tgtcatgcag cggcttgaat
     4801 tcgctgctgg tgtcccacca cttgtgggcc acctggctga atttttccag ttcagcctga
     4861 tcgacgttgc tcatcgtc
//
LOCUS       DAOPYX010000225         4875 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_22086_length_4875_cov_7.569917, whole genome shotgun sequence.
ACCESSION   DAOPYX010000225 DAOPYX010000000
VERSION     DAOPYX010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 4875)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4875)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4875
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22086_length_4875_cov_7.569917"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            284..2473
                     /locus_tag="PKN55_10925"
     CDS             284..2473
                     /locus_tag="PKN55_10925"
                     /inference="COORDINATES: protein motif:HMM:NF018010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HNX71799.1"
                     /translation="MMRMRVFLLVLASLLSLCAPVTGWAAAASQDPRSTITGSSPIDA
                     TSGTAVRTPAGSGSTTGKPDAPPPEPPLEFQSFVSQASGLGLPVYGHDFFARASSSMG
                     APLEDAPLSADYVIGPGDEVLIRAWGQVDIDYRAKVDRAGNINVPKVGVLHVAGTEYR
                     QLNAYLRTAISRVFRNFELNVSLGELRSIQVFVVGHARYPGTYLVSSMSTVLSALFAT
                     GGPSNKGSLRRIYLRRNNQVVAEFDLYDFLLRGDKSRDMRLLPGDVIQIPPVGGLAAV
                     VGSINTQAIFELKKGESLAYLLGAAGGLTTTAAGQKVTLERIVERTLRKVEEFTLDTD
                     GLARQVRDGDIVRVYAISPRIENVVTLRGNVATPANVPWRKGMRISDLIPERAALLSP
                     DYWNARNAAVREAVGRESAFKPRVTGELNWNYAVIERMHPTTQAVTLIPFNLAKAVLQ
                     RDPSANLVLEPGDVVTVFGREDLRQPVDSNDRFVRLEGEVRAPGLYRLLPGETARQLV
                     KRLGGVTPNAYLFGAELTRASALGTQQKRLDELLARLDVQMSFETVVASRNNTGEAGG
                     KDEADIRKNLLESLRKLPATGRVLLHLEPSIGSIEAFPDFPLEDGDRLYFPPVTKTIN
                     VVGAVLKEMPLSYQPGLGLSGYLDKLGGMTKEADTSAIYVQRADGSVWSRHQSFWPLS
                     SVSIMPGDTIVVPYETDRTLWRKFLRDWAQIFYQFGISVAAIHAVTK"
     gene            2482..3789
                     /locus_tag="PKN55_10930"
     CDS             2482..3789
                     /locus_tag="PKN55_10930"
                     /inference="COORDINATES: protein motif:HMM:NF014732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNVR domain-containing protein"
                     /protein_id="HNX71800.1"
                     /translation="MSSQESMPLDEESVAQMPQEPALVEPKFHPLDVILSLLRYKRWL
                     VICPLIAAVIAFAFVSTQLPRYSAMARLLPPKTKPNTATMLSQTGSLAGAGVDGVLEG
                     QKPVVDMYLAILQSRAAAEFMDSNFDLRKRWGVSSIESVYGRLSSMSKIAPSPNGIIN
                     IEIEDSDPVWAARLANGYADALQYITQGLAVNEASKRRVFYEQQMNVSLQQLTVAQMS
                     LRKIQQETGVLNLDPQIQMAIQESNRLRTAIASKETELASMEAFATERNADYLRLQHE
                     IDALKNQLEQWRRPVKASDDVALQASEGLLPEISMEYVKHVREVKNLELVYELFLKEL
                     QLTRIDQVKDATTVELLDKAIPPSTPSYPKKKRTIAIASLIGFLIAMVSILIYELTVV
                     ALKNEVNMSRVRMIREALRWSPPSVRFPRKLKFNVKFKRKNKE"
     gene            3786..4853
                     /gene="rfbB"
                     /locus_tag="PKN55_10935"
     CDS             3786..4853
                     /gene="rfbB"
                     /locus_tag="PKN55_10935"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019921345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HNX71801.1"
                     /translation="MNGVIVVTGGAGFIGSNFVREWFAHVPDARVVVLDALTYAGHLE
                     SLEAVKNEPGFAFVQGDIADSACVNALIAQYQPVAIVNFAAESHVDRSIHGPGDFIRT
                     NVNGTFTLLEAARAHWSALQGEARDNFRFLHVSTDEVYGSLEPGDAPFTETHPFQPNS
                     PYSASKAASDHLVRAWFHTYGLPVLTTNCSNNYGPFHYPEKLIPLVILNALAGKPLPI
                     YGDGSNIRDWLFVTDHCAAIRRVLAAGQLGETYNVGGWNEKTNLEVVHTICAQLDALH
                     PREDGRAYAEQITFVTDRPGHDRRYAIDASKLHRELGWKPAETFETGVAKTVRWYLDN
                     QDWVRAVTGGAFDAWLKNHYK"
BASE COUNT         1008 a         1297 c         1417 g         1153 t
ORIGIN      
        1 tgcaaacctt catcaacgcc gggcaactgc cggagcacac catcaagtgg cagctgttcc
       61 tgcatctggc tttcctggtg tcggcagcgg cgattgcgta taccgacaag ttgttgcatg
      121 tggcgcacgc aaaagcatcc cactgagtcg cgcgatccac cctgctgaga ggggggatat
      181 gccgatgtct ggctgttgaa tttttttata tatgaatggc atggtcgcgg cttcccattt
      241 tgtaaagtga tcatgctaag attggcttcc tgaatgtgtg cccatgatgc gtatgcgtgt
      301 ttttctgctc gtccttgctt cactgttgtc gctatgtgcg ccggtcacag gatgggctgc
      361 ggccgcttct caggatccac gctccacgat caccggcagc tctccaattg atgcgacctc
      421 cggaaccgca gtgcgtacac ctgcaggttc tggttctacg accggcaagc cagatgcgcc
      481 acccccggag cctccgctgg aatttcagtc ctttgtttcc caggccagtg gtctcggctt
      541 gccggtatac gggcatgact tctttgcccg ggcttcttcg tccatgggcg ctccgttgga
      601 agatgcccct ctgtccgctg actatgtgat cgggccgggg gatgaggtgc tgatccgtgc
      661 ctggggccaa gtggatatcg actatcgggc caaagtggac agggccggca atatcaatgt
      721 ccccaaggtg ggggtattgc atgttgctgg cacagagtac cgtcagctca atgcatattt
      781 gcgaacggct atttcccgcg tatttcgcaa ctttgaactg aacgtttcct tgggtgaatt
      841 gcgctcgata caagtgtttg tggtcggcca tgcccgttat cctggcactt atctggtcag
      901 ttcaatgagt acggtgctca gtgccctgtt tgccactggt gggccgtcta ataagggctc
      961 cttacgtcgt atttacctgc gccgaaacaa tcaggtggtg gcagaatttg acctctacga
     1021 ctttttattg cgtggcgaca agagtcgcga catgcgcctg ctgccggggg atgtgatcca
     1081 gatccctccc gtgggcgggt tggctgccgt ggtcggcagt atcaataccc aggctatttt
     1141 tgaattgaaa aaaggtgagt ccttggctta tctgctgggg gccgctggag gtttgaccac
     1201 caccgctgcc gggcagaaag tgacactgga gcgtattgtc gagcgcacct tgcgcaaggt
     1261 ggaagagttt acgctggaca ctgacgggct ggctcgtcaa gtgcgtgacg gcgatatcgt
     1321 cagggtatat gcgatttcgc cgcgcatcga aaatgtcgtg accctgcgtg gcaatgtggc
     1381 gactcccgcc aatgttccct ggcgcaaggg catgcgcatc agtgacttga ttcctgaacg
     1441 ggccgctttg cttagccctg attactggaa tgcgcgcaat gcggcggtac gtgaagcggt
     1501 gggccgtgaa agtgcgttca agcccagggt gaccggtgag cttaattgga attacgccgt
     1561 cattgagcgt atgcatccta ctacccaggc cgtgacattg atcccgttca acttggcgaa
     1621 ggctgtgctg caacgtgatc cgtctgccaa tctggtcttg gagccgggtg atgtggtgac
     1681 tgtattcggc cgtgaagatt tgcgccagcc ggtggacagc aatgaccgtt ttgtccgtct
     1741 ggaaggcgag gtgcgtgcgc ctggcctgta tcgcttgtta cctggtgaga cagccagaca
     1801 actggtcaaa cgtctgggcg gggtgacgcc caatgcatat ctgtttggcg ccgaattgac
     1861 tcgggcttct gcattgggca cccagcaaaa acggctggat gagttgctgg ctcgtctgga
     1921 tgtgcagatg agctttgaaa ctgtggtggc ttctcgcaac aacaccggtg aagccggggg
     1981 caaggacgag gcggatattc gcaagaatct gttggaatca ctgcggaaat tgcctgccac
     2041 cggccgggtc ttgctgcatc tggagccttc tattggctcg attgaagcat ttcctgactt
     2101 tccgctggaa gatggcgacc gtctgtattt tcctcctgtg accaagacca tcaatgtggt
     2161 tggcgcggtg ctaaaagaaa tgcctttgtc gtatcaacct ggcctgggtt tgtctggcta
     2221 cctggataag ctgggcggca tgaccaaaga ggctgatacg agcgccattt acgtccagcg
     2281 tgcagatggt tcggtatgga gccgtcacca gtcattctgg ccattatcgt cggtcagcat
     2341 catgcctggt gacaccattg tcgtgccgta tgaaactgac cgcaccctgt ggcgcaaatt
     2401 cctgcgcgac tgggcacaga ttttttacca gttcgggatc agcgtcgctg ccatccatgc
     2461 cgtgacgaag tgagtctggc tatgtcttct caagagtcca tgccgctgga tgaagaaagc
     2521 gtggcccaga tgccgcaaga gcctgcgttg gtggagccca agtttcaccc gctggacgtc
     2581 attctttcac tgttgcgcta taagcgttgg ctggtgattt gcccattgat tgcggcggtc
     2641 attgcatttg cctttgtctc tacacagttg ccacgctatt ccgccatggc tcggctgttg
     2701 ccgccgaaaa ccaaacccaa caccgccacc atgctcagcc agacaggttc cctggcaggc
     2761 gccggggtgg atggggtgct ggaagggcaa aagcccgtgg tggacatgta tctggcgatt
     2821 ttacaaagcc gggccgcggc agaattcatg gacagcaatt ttgatctgcg caaacgctgg
     2881 ggcgtctcgt cgatcgaatc ggtgtatggc cgcttgtcca gcatgtccaa gattgcgccc
     2941 agcccgaacg ggatcatcaa tattgaaatt gaggacagtg accccgtctg ggcggccagg
     3001 ctggccaacg ggtatgctga cgccttgcaa tacatcacgc aaggcttggc ggtcaatgaa
     3061 gcatccaagc gacgggtgtt ctatgagcag caaatgaatg tttccttgca gcagttgacc
     3121 gtggcccaaa tgtcattgcg caagattcag caggaaaccg gggtattgaa tctggacccg
     3181 caaatccaga tggcgattca ggaaagtaac cgtttgcgca ctgccattgc cagcaaagaa
     3241 acggaactgg catccatgga ggcgtttgcg accgaacgta atgctgacta cttgcgtctg
     3301 cagcacgaga ttgacgcatt gaaaaaccag cttgagcaat ggcgccgccc cgtaaaagcc
     3361 agtgatgatg ttgccttgca ggcatcagaa ggcttgctgc ctgaaatttc catggagtac
     3421 gtcaagcatg ttcgtgaggt caagaatctg gagttggtgt acgagctgtt cctgaaggaa
     3481 ctgcaattga cccgaattga ccaggtgaaa gatgccacaa cggttgagtt gctggataaa
     3541 gccattccgc cttccacgcc ttcgtatccc aagaaaaagc gcacgatcgc cattgcgagc
     3601 ctgatcggtt tcttgatcgc gatggtctcc attctgattt atgaattgac cgtggtggcg
     3661 ctcaagaatg aagtcaatat gagccgggtg cgcatgattc gcgaagcatt gcgctggtcg
     3721 ccgccatcgg taagattccc tcgcaaattg aagttcaatg tgaaattcaa aagaaagaat
     3781 aaagaatgaa tggcgtgatt gtggtcaccg gtggggccgg tttcattggc agtaactttg
     3841 tgcgcgaatg gtttgcccat gtgcccgatg ctcgcgtggt agtgctggat gcattgacct
     3901 atgccggtca cctggaaagc ctggaggccg tcaaaaatga acctggcttt gccttcgtgc
     3961 agggtgatat tgccgatagc gcctgcgtca atgccctgat cgcccagtat cagccggtag
     4021 ccatcgtcaa ttttgccgct gaaagccatg tggatcgttc catccatggc ccgggtgatt
     4081 ttatccgcac caatgtcaac ggcaccttta ccctgctgga ggccgcgcgt gcccattggt
     4141 cggcgctgca aggtgaggca cgggacaatt tccgtttcct gcatgtgtct accgacgaag
     4201 tgtacggctc gctggagccc ggtgacgcgc cgtttaccga aacgcatccg tttcagccca
     4261 atagcccgta ttccgcctct aaagcggcct cagatcatct ggtgcgtgcc tggttccaca
     4321 cctatggcct gcccgtgctg accaccaatt gctcgaacaa ttatggcccg tttcactacc
     4381 cggaaaaact gattccgcta gtgatcctca acgccctggc cggcaagccg ctgccgattt
     4441 acggcgatgg cagcaatatc cgcgactggc tgtttgtcac cgaccactgc gcggccattc
     4501 gtcgcgtgct ggccgccggc cagttggggg aaacctataa cgtgggcggc tggaacgaaa
     4561 aaaccaatct ggaagttgtg cacaccatct gcgcccagct ggatgccttg catccgcgcg
     4621 aggatggccg cgcttatgcc gagcagatta cttttgtgac cgaccgccca ggccatgacc
     4681 gccgctatgc catcgacgcc agcaagctgc atcgcgaact gggttggaaa ccggcagaaa
     4741 ccttcgaaac cggggtggcc aaaaccgtgc gctggtatct ggacaaccag gactgggtgc
     4801 gcgccgtcac cggtggggca ttcgatgcat ggctgaaaaa tcattacaaa taatcacagg
     4861 aatacgcaga tgaca
//
LOCUS       DAOPYX010000226         4836 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_22346_length_4836_cov_12.915290, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000226 DAOPYX010000000
VERSION     DAOPYX010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 4836)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4836)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4836
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22346_length_4836_cov_12.915290"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(248..2689)
                     /locus_tag="PKN55_10940"
     CDS             complement(248..2689)
                     /locus_tag="PKN55_10940"
                     /inference="COORDINATES: protein motif:HMM:NF025214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNX71802.1"
                     /translation="MSTPSIQTRLQAALALQRAGQFAQAIDDYLDILSQHPDQHDAHH
                     QLGVAYWNHGQQEVALTHLQRALQAPQVPGGYWLAVVDALTGLGRHSEAQHWLVKARQ
                     RGLPASELLGRAQATNPPPEVQEQMFAHMQREELDACRALTHPLLAQYPAFGQGWKML
                     AYLFQLNGEYQPALEAGQQATFWLPDDPEVFNNLGISARSVGELALAEQSYRQALALD
                     PNRVDTHYNLSALLYEQGRRREAEPPCRRALELRPNYPAALNNLANMLRERGELGEAE
                     AVCRQALALKPDYVLAHNNLGIILHGQDRSDEAEASYRRALALAPTDLKSLNNLGNLL
                     KSRGRLNEAADCYRAALQQAPDMAELHGNLANVQKDLGELDAALAGFSHAIRLKPAQA
                     DTWHNLLLTLQYADHARAADCAHWHRAFAEQFEAPWRGRVAAHPNSREPERRLRVGWV
                     SADFCNHSVSYFTLPLLQALAARPDLGLELYGYSQVLVHDPLSKQLEACCAKWRHVLG
                     LNDDELEDCIRRDQIDILIDLSGHTANNRLPVFARKPAPVQVGWLGYPDSSGLTHIDW
                     RITDRHGDPEGADEYYSEKLWRLPEVFCCYRPMIRFPEKRQHPDYAVWPTPALETGYL
                     TFGCCNNIAKLTPTTLKLWAKVLHAVPNSRLLLELAGLEQSTLSHKLLADFAALDVPA
                     ERLQLIHRDRAWQYRRYQQIDIVLDPFPANGGTSSCDLLWMGVPLVTLSGERFVSRMG
                     ATLLHAVGHPEWIAHTAEEYVQIAQRLAADVPALNAIREGLRAETEASPLMDEAGFAE
                     VFAGALRGMWRGWCK"
     gene            complement(2704..3516)
                     /locus_tag="PKN55_10945"
     CDS             complement(2704..3516)
                     /locus_tag="PKN55_10945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014088529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3025 domain-containing protein"
                     /protein_id="HNX71803.1"
                     /translation="MFTAPADWSTAWLRRPAYAVWAAVAQPLAAQTDWPEQAALDALF
                     AQARAAGCPLPPGLRCVLDLQPDAYYESHIAATGQVPTRARNWHDLFNALAWLAWPRL
                     KTALNARHQAAIAAGEAKRGPIRDSATLLDECGLLMPYAERTLAEAVDGMRWGELWQD
                     RRADWGRLIDAYPLGHALPEMLLAPFIGLTGKAWLLPVDASFFTLPFPTRLARLDALA
                     AERLSTLDRPSRLAPLPVLGIPGWHAGAQDAGFYANADYFRPSRRAPSVWAA"
     gene            3686..>4836
                     /gene="cysG"
                     /locus_tag="PKN55_10950"
     CDS             3686..>4836
                     /gene="cysG"
                     /locus_tag="PKN55_10950"
                     /EC_number="1.3.1.76"
                     /EC_number="2.1.1.107"
                     /EC_number="4.99.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014088527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0043115 - precorrin-2 dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051266 - sirohydrochlorin ferrochelatase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siroheme synthase CysG"
                     /protein_id="HNX71804.1"
                     /translation="MDFLPIFMKMRGAPALVVGGGDVALRKARLLHEAGCRLTIVAPE
                     CHPELAEWIASGGVRQLARGFMPEDIAGQRLVIAATDDEAVNAQVSALCMAQNIPVNV
                     VDNPRLCSFIMPSIIDRSPVIVAISSSGGLPVLARWLRTQIEALLPAGLARLGRFGQE
                     VRPRLKTAMPDANQRRGFWERLLASPWAEQMMNGDEARARADFDRALAEHNAPPVGAV
                     YLVGGGPGNPDLLTFRALRLMQQADVVLYDNLVAPEIVELTRRDADRIYVGKRASQHT
                     LPQEDINSLLVRLAKEGKRVVRLKGGDPFMFGRGGEEIETLAEHGIPFEVVPGITSAN
                     GAAAYAGIPLTHRDHAQSCVFVTGHRRDGSIDLDWAAITRPQQTVVIYMG"
BASE COUNT          737 a         1553 c         1700 g          846 t
ORIGIN      
        1 gctggcaatc ataccagact gcgggcgggg tgggcggatg gcgcacagca acaccgctta
       61 cccgcccagc gtcttggcgc acgctgccaa cggcttgagc aggtcggcgt ggatggcgtc
      121 gcgcatgccg ggcatcgaca gcagggcctg ggtttcctga acggcgcaga tgtgctctct
      181 tggctggcat ggtggtgttt tgtttgtcag tgtttgcttt gtctgcatcg tatgcatgca
      241 agaaatacta cttacaccac ccccgccaca tccccctcaa cgcccccgca aacacctcag
      301 caaaccccgc ctcatccatc agcgggctgg cttctgtttc tgcccgcagt ccttcgcgga
      361 tggcgttcag cgccggcaca tccgccgcca gccgctgggc gatctgcaca tattcttctg
      421 cggtgtgggc gatccattcc gggtggccga cggcgtgcag cagggtggcg cccatgcgtg
      481 agacaaagcg ttcgccgctg agggtgacca gcggcacgcc catccacagc aggtcgcagc
      541 tggaggtgcc gccgttggcg gggaaggggt cgagcacgat gtcgatttgc tggtagcggc
      601 ggtattgcca ggcgcggtcg cggtggatca gttgcaggcg ttcggcgggc acgtccaggg
      661 cggcaaagtc ggccagcagt ttgtggctga gggtgctttg ctccagcccg gccagttcca
      721 gcaataggcg gctgttgggc acggcgtgca gcactttggc ccacagcttg agcgtggtgg
      781 gggtgagttt ggcgatgttg ttgcagcagc caaaggtgag gtagccggtt tccagcgcgg
      841 gcgtgggcca caccgcgtag tcggggtgct ggcgtttttc cgggaagcgg atcatcggcc
      901 ggtagcagca aaacacctcg ggcaggcgcc agagtttttc gctgtaatac tcgtcggcgc
      961 cttcggggtc gccgtggcgg tcggtgatgc gccagtcgat gtgggtcagc ccgctgctgt
     1021 ccgggtagcc cagccagccc acttgcactg gcgcgggttt gcgggcgaac accggcaggc
     1081 ggttgttggc ggtgtggccg gagaggtcaa tcaggatgtc gatctgatcg cggcggatgc
     1141 agtcttccag ctcgtcgtcg ttcaggccca gcacatgccg ccatttggcg cagcaggctt
     1201 ccagctgttt gctcagcggg tcgtgcacca ggacctggct gtagccgtac agctccaggc
     1261 ccaggtcggg gcgggcggcc agtgcttgca gcagcggcag ggtgaagtag ctgaccgagt
     1321 gattgcaaaa atccgccgac acccagccca cgcgcagccg gcgttctggc tcacggctgt
     1381 tggggtgggc ggccacgcgg ccgcgccagg gggcttcaaa ttgctcggca aaggcgcggt
     1441 gccagtgggc gcagtcggcg gcgcgggcgt ggtcggcgta ttgcagggtg agcagcagat
     1501 tgtgccaggt gtcggcttgg gctggtttga ggcggatggc gtggctaaag ccggccagcg
     1561 cggcgtccag ctcgcccagg tctttttgca cattggccag gttgccgtgc agctcggcca
     1621 tgtccggcgc ttgttgcagc gcggcgcggt agcagtcggc ggcttcgttc agccggccgc
     1681 gcgatttcag cagattgccc aggttgttca gcgatttcag gtcggtgggg gccagcgcca
     1741 gcgcgcgccg gtagctggct tcggcctcgt cgctgcggtc ctggccgtgc aggatgatgc
     1801 ccagattatt gtgcgccagc acatagtccg gcttgagcgc cagcgcctgc cggcacacgg
     1861 cttcggcttc gcccagctcg ccgcgctcgc gcagcatatt ggccaggttg ttcagcgcgg
     1921 ccgggtagtt ggggcgcagt tccagcgcgc ggcggcacgg cggctcggcc tcgcggcggc
     1981 ggccttgttc gtagagcagc gcgctcaggt tgtaatgggt gtccacccgg ttaggatcca
     2041 gcgccagcgc ctgacgatag ctttgctcgg ccagcgccag ctcgcccacg ctgcgcgcgc
     2101 tgatgcccag attgttgaac acttccgggt cgtcgggcag ccagaatgtc gcttgctggc
     2161 cagcttccag cgccggttgg tattcgccgt tcagttgaaa cagataggcc agcattttcc
     2221 agccctggcc aaaggccggg tattgcgcca acagcgggtg ggtcagcgcg cggcaggcgt
     2281 ccagttcttc gcgctgcata tgggcgaaca tctgttcctg cacctctggc ggcgggttgg
     2341 tggcttgcgc ccgcccgagc agctcgctgg ccggcaggcc gcgctggcgc gccttcacca
     2401 gccagtgctg cgcctcgctg tgccggccca ggccggtgag cgcgtccacc accgccagcc
     2461 aatacccgcc cggcacttgt ggcgcctgca aggcgcgctg caagtgggtc agcgccactt
     2521 cttgctggcc gtggttccag taggccacgc ccagttgatg atgggcgtcg tgctggtcgg
     2581 ggtgttggct gaggatgtcc agatagtcgt cgatggcttg ggcaaactgg ccggcgcgtt
     2641 gcagcgccag cgcggcttgc agacgggttt ggatcgaggg cgtgctcatg gtgtgctcac
     2701 ggttcaggct gcccacaccg acggcgcgcg ccggctgggt cgaaaataat ctgcattggc
     2761 gtaaaacccg gcgtcctgcg cgccagcgtg ccagccgggg atgcccagca cgggcagggg
     2821 cgccagccgc gaggggcggt ccagcgtgct caggcgctcg gccgccaggg catccagccg
     2881 ggccagccgg gtagggaagg gcagggtaaa aaagctggcg tctaccggca gcagccaggc
     2941 tttgccggtc aggccgataa acggcgccag cagcatctcc ggcagtgcat ggcccagcgg
     3001 ataggcgtcg atcagccggc cccaatcggc gcggcggtct tgccataatt ctccccagcg
     3061 catgccgtcc accgcctcgg ccagcgtccg ctcggcgtag ggcatcaaca gcccgcattc
     3121 gtccagcaag gtggcgctgt cgcggatcgg cccgcgcttg gcttcgccag cggcgatggc
     3181 cgcctgatgg cgggcgttca gcgcggtttt caggcgcggc caggccagcc aggccagggc
     3241 gttgaataaa tcatgccagt tgcgcgcgcg ggtgggcact tgcccggtgg cggcgatatg
     3301 gctttcgtaa taggcgtcgg gctgcaaatc cagcacgcag cgcaggccgg gcggcagcgg
     3361 gcagccggcg gcgcgcgcct gggcaaacag cgcatccagc gccgcttgct cgggccagtc
     3421 ggtttgcgcc gccagcggct gggccaccgc cgcccacacg gcgtaagcgg ggcgcctcag
     3481 ccaggcggtg gaccaatcgg caggggcggt gaacacctgg ggcaaggcaa tctcgttcag
     3541 gacaatggca atggccgtta tcgtggccga tgtggccaag gccgtcaaat cgccgccagc
     3601 gcagccacgg cgcccggcaa tgctatgatg aaatccccaa attcaatatt ccgcctggcc
     3661 cgtccggcgg caagagcagc gtgcgatgga ctttttaccg attttcatga aaatgcgcgg
     3721 cgcgcccgcg ctggtggtgg gcggcggcga tgtggccttg cgcaaggcgc ggctgctgca
     3781 cgaggccggc tgtcggctga ccatcgtcgc gccagaatgc caccccgagc tggccgagtg
     3841 gatcgccagc ggcggcgtgc gccagctggc gcgtggcttt atgcccgagg atatcgccgg
     3901 ccagcgcctg gtgattgccg ccaccgacga tgaagccgtc aacgcccagg tcagcgcgct
     3961 gtgcatggcg caaaacatcc cggtgaacgt ggtggacaac ccgcggctgt gcagctttat
     4021 catgccgtcg attatcgacc gctcgccggt gatcgttgcc atttcctcgt ccggcggtct
     4081 gccggtgctg gcgcgctggc tgcgcacgca aatcgaggcg ctattgccgg ccggcctggc
     4141 gcggctgggc cgctttggtc aggaagtgcg cccgcgcctg aaaaccgcca tgcccgacgc
     4201 caaccagcgc cgtggctttt gggagcgcct gctggccagc ccctgggccg agcaaatgat
     4261 gaacggcgac gaagcccgcg cccgcgccga tttcgaccgc gcgctggccg agcacaacgc
     4321 cccgccagtc ggcgcggtgt atctggtggg cggcggcccc ggcaacccgg atttgctcac
     4381 cttccgcgcg ctgcggctga tgcagcaggc cgacgtggtg ctgtacgaca atctggtggc
     4441 cccggaaatc gtcgagctga cccgccgcga cgccgaccgc atttatgtcg gcaagcgcgc
     4501 cagccagcac accctgccgc aagaagacat caacagcctg ctggtgcgtc tggccaaaga
     4561 aggcaagcgc gtggtgcgcc tcaagggcgg cgaccccttc atgtttggcc ggggcggcga
     4621 agaaatcgaa accctggccg aacacggcat cccgtttgaa gtggtgcccg gcatcacctc
     4681 ggccaacggc gcggcggcat acgccggcat cccgctcacc caccgcgacc acgcgcaatc
     4741 ctgcgtgttt gtcaccggcc accgccgtga cggcagcatc gacctggact gggccgccat
     4801 cacccgcccg cagcaaaccg tggtgatcta tatggg
//
LOCUS       DAOPYX010000227         4826 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_22412_length_4826_cov_11.291344, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000227 DAOPYX010000000
VERSION     DAOPYX010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 4826)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4826)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4826
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22412_length_4826_cov_11.291344"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1334
                     /locus_tag="PKN55_10955"
     CDS             <1..1334
                     /locus_tag="PKN55_10955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019922244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HNX71805.1"
                     /translation="TPDPGVIEVNIHPASHWEELVERTEYLYQAAHETRLSTEKFMLD
                     GRHTGTGGGNHFVLGGATPADSPFLRRPDVLASLIAYWHNHPSLSYLFSGLFIGPTSQ
                     APRLDEARNDQVYEMDIALQEIRKAVARQGADVPPWLIDRILRNLLVDVTGNTHRAEF
                     CIDKLYSPDSTTGRLGLLELRAFEMPPHARMSLAQQLLIRALVARFWRTPYQAGLTRW
                     GTGLHDRFMLSTFVQMDFEDVLDELNQAGYAFELAWFAPHFAFRFPRMGELNVRGIHL
                     ELRHALEPWHVMGEEGMAGTTVRYVDSSLERVEVKVSGLNDNRYVITVNGQPLPLQPT
                     GRVGEYVAGVRYRAWNPPSALHPTVGVHAPLVFDVIDTWSERSLGGCQYHVAHPGGRN
                     YASFPVNAYEAESRRLARFFTTGHTAGSVTVSARAPSREHPFTLDLRRQPG"
     gene            1409..3940
                     /locus_tag="PKN55_10960"
     CDS             1409..3940
                     /locus_tag="PKN55_10960"
                     /inference="COORDINATES: protein motif:HMM:NF025761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HNX71806.1"
                     /translation="MNSDYAADAARACDHFDEWQDAHGVRMHWRRFFELESAVELAGK
                     QGDADRQIRDNGITYNVYADTRLGPARPWSLDILPFILDDADWRHIASGVSQRARLLN
                     QMLADLYGEQTLLQQGVLPTELVLGHPGYVRALKGHRPAGGVFLHIVAFDLARHPNGR
                     WCVVSQRTQAPSGLGYALENRLIVSRLFPEAFRQLRVQHLASSYRQLLATLSQLSPPT
                     PRQSAPRMVLLTPGPYNETYFEHAYLARYLGIPLVEGSDLTVRGDELFLKTLTGLTPV
                     QVVLRRLDDNYCDPLELRSDSTLGVPGLLQAVRAGKVLVANALGSSFLESPGLHAFLP
                     GVSQALLGEPLQLPSLLSWWCGEDAALVEALECLPQRVIKPTYPAASPREHMEPVLAS
                     QLDAASLHAWRERILRQPGDFTLQENVPLSQAPIWRHGHIAAHPAMLRVFAIADGQGG
                     WQVMPGGLTRVADEPDQPVVSMQRGGSSLDTWVLTQGEVDTFSLLPGQLRPTDLAHKR
                     RLVTSRAAENLFWMGRYAERAEQRVRLVYWALEWLAVRQTDSAGNDACLPLLENLCRY
                     MGLLPVGTPAGIGPEALARALRIGLVDGQAAWSVAHTLSALAGAAAPVRDRLSSTYSR
                     LVLATSRRFSGQQAQRAHQGDYSNAEALADLEQLAVELVAIAGEQADHMTRDDGWRFL
                     AIGRHLERLNLLAAQLGLGIAGCTPPQGPAFELLLALFDSTLTYRSYYQCRQELPALL
                     DLLVCDANNPRAIRSVQAALRNELEALPKLPGAPALAEWLPSSAPGLDALCQRAPNQQ
                     LETLRCFLQHLDNASARLSDEIGLHYFNHNPSWHPDNPPARNSPC"
     gene            3934..>4826
                     /locus_tag="PKN55_10965"
     CDS             3934..>4826
                     /locus_tag="PKN55_10965"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HNX71807.1"
                     /translation="MLNTADTVLHISHVTHYDYASRVDLAMHLLHLQPLSRADQQLED
                     FRLDIDPPATRNQASLDYFGNPQHHLTLTTPHHALTVRAESRVRRLAAATPKPSASPA
                     WESVRAAMRYRAGQPWLPAAEFCFPSAFVPLHPAFQAYAQLEFTPGRPLLEAAIGLMA
                     RIHHEFTYATASTDLTTPALEAFSRRQGVCQDFAHIMIACLRSLGLPARYVSGYLLTQ
                     PPPGQPRLLGVDASHAWLAVWCPQHDWVELDPTNNLIAAQSHAVIACGRDYADVAPLR
                     GVIQGGGSHTLSVAVSVVPEG"
BASE COUNT          763 a         1765 c         1542 g          756 t
ORIGIN      
        1 tcacccccga ccccggcgtg atcgaggtga acatccaccc ggccagccac tgggaagaac
       61 tggtcgagcg caccgagtat ttgtatcagg ccgcgcacga aacccggctg tccaccgaga
      121 aattcatgct cgacggccgc cacaccggca ccggcggcgg caaccacttt gtgctgggcg
      181 gcgccacccc ggccgacagc cccttcctgc gccgcccgga cgtgctggcc agcctgattg
      241 cctactggca caaccacccc tcgctgagct atctgttctc tggcctgttt atcggcccga
      301 ccagccaggc gccgcgactg gacgaggcgc gcaatgatca ggtgtacgag atggacatcg
      361 ccttgcagga aatccgcaag gcggtggccc gccagggcgc cgacgtgccg ccgtggctga
      421 ttgaccgcat cttgcgcaat ctgctggtgg acgtcaccgg caacacccac cgcgccgaat
      481 tctgcatcga caagctgtat tcgccggatt ccaccactgg ccggctgggc ctgctggagc
      541 tgcgcgcctt tgaaatgccg ccgcacgcgc gcatgagcct ggcgcaacag ctgctgatcc
      601 gcgcgctggt ggcgcgcttc tggcgcacgc cgtatcaagc cgggctgacc cgctggggca
      661 ccggcctgca cgaccgcttt atgttgtcca cctttgtgca gatggacttc gaagacgtgc
      721 tggacgagct taatcaagcc ggctacgcct ttgagctggc ctggtttgcc ccgcactttg
      781 ccttccgctt cccgcgcatg ggcgaactga atgtgcgcgg catccatctg gagctgcgcc
      841 acgcgctgga gccctggcat gtgatgggcg aggaaggcat ggccggcacc acggtgcgct
      901 atgtggattc gtcgctggag cgcgtcgagg tcaaggtcag cgggctgaac gacaaccgct
      961 atgtcatcac cgtcaacggc cagccgctgc cgctgcaacc caccggccgg gtgggcgaat
     1021 atgtggccgg cgtgcgctac cgggcgtgga acccaccctc ggcgctgcac ccgaccgtcg
     1081 gcgtgcacgc gccgctggtg tttgacgtga tcgacacctg gagcgaacgc tcgctgggcg
     1141 gctgccaata ccatgtcgcc catccgggcg ggcgcaacta cgccagcttc ccggtgaacg
     1201 cctacgaggc ggaaagccgc cgcctggcgc gcttcttcac cactggccac accgccggct
     1261 cggtgacggt cagcgcgcgc gcgcccagcc gcgagcatcc cttcaccctc gatttacgcc
     1321 gccagccggg gtagactttc gccctgacct atcctgaccg aaccacgccc ggctgaccgc
     1381 cgggcgtttt ttccctagac aatatggcat gaacagcgat tacgccgcag acgccgcccg
     1441 cgcctgcgac cacttcgacg aatggcagga tgcccacggc gtgcgcatgc actggcggcg
     1501 attctttgag ctggagtccg ccgtcgagct ggccggcaag caaggcgacg ccgaccggca
     1561 aatccgcgac aacggcatca cctacaatgt ctacgccgac acccggctag gcccggcgcg
     1621 gccgtggtcg ctggacatcc tgccctttat cctcgacgac gccgactggc ggcacatcgc
     1681 cagcggggtc agccagcgcg cgcgcctgct caaccagatg ctggccgacc tgtacggcga
     1741 acaaaccttg ctgcaacaag gcgtgctgcc caccgagctg gtgctgggcc accctggcta
     1801 tgtgcgcgcg ctcaagggcc atcgcccggc cggcggggtg tttttgcata ttgtggcgtt
     1861 tgatctggcc cgccatccca atggccgctg gtgcgtggtg tcgcagcgca cccaggcgcc
     1921 gtccgggctg ggctatgcgc tggaaaaccg gctgattgtc tcgcggctgt ttcccgaggc
     1981 gtttcgccag ctgcgcgtgc agcatctggc cagcagctac cggcaattgc tggccaccct
     2041 gtcgcagctg agcccgccca ccccgcgcca gagcgcgccg cgcatggtgc tgctcacccc
     2101 cggcccgtat aacgaaacct attttgagca cgcttatctg gcgcgctatc tgggcatccc
     2161 gctggtggaa ggcagcgacc tcaccgtgcg cggcgacgag ctgttcctga aaaccctcac
     2221 cggcctgacc ccggtgcaag tggtgctgcg ccggctggac gacaattatt gcgacccgct
     2281 ggaactgcgc agcgactcca ccctgggcgt gcccggcctg ttgcaggcgg tgcgcgccgg
     2341 caaggtgctg gtggccaatg cgctgggcag cagctttctg gaaagccccg gcctgcacgc
     2401 ctttttgccc ggcgtcagcc aggcgctgct gggcgagccc ttgcagctgc cctcgctatt
     2461 gtcctggtgg tgcggcgaag acgccgcgct ggtcgaagcg ctggaatgcc tgccgcaacg
     2521 ggtgatcaag cccacctacc cggccgcctc gccgcgcgag catatggagc cggtgctggc
     2581 cagccagctg gacgccgcca gcctgcacgc ctggcgcgag cgcatcctgc gccagccggg
     2641 ggatttcacc ctgcaagaaa acgtgccgct gtcgcaagcg ccgatctggc ggcacggcca
     2701 catcgccgcc cacccggcca tgctgcgggt gttcgccatc gccgacggcc agggcggctg
     2761 gcaggtgatg cccggcggcc tcacccgcgt ggccgacgag cccgaccagc cggtggtgtc
     2821 gatgcagcgc ggcggcagca gcctggacac ctgggtgctg acgcaaggcg aggtggacac
     2881 gttttcttta ttgcccggcc agctgcgccc caccgacctg gcgcacaagc gccggctggt
     2941 gaccagccgc gccgccgaaa acctgttctg gatgggccgc tacgccgagc gcgccgagca
     3001 gcgcgtgcgg ctggtgtact gggcgctgga atggctggcg gtgcgccaga ccgacagcgc
     3061 cggcaacgac gcctgcctgc cgctgctgga aaacctatgc cgctatatgg ggctgttgcc
     3121 ggtgggcacg ccggccggca tcgggccgga agcgctggcg cgcgcgctgc gcattggcct
     3181 ggtggacggc caggcggcct ggagcgtcgc ccacaccctg agcgcgctgg ccggcgccgc
     3241 cgcgccagtg cgcgaccggc tgtcatccac gtattcacgg ctggtgctgg ccaccagccg
     3301 gcggttttct ggccagcagg cgcagcgcgc ccaccagggc gactacagca atgccgaggc
     3361 gctggccgac ctggagcaac tggcggtcga actggtggcg attgccggcg agcaggccga
     3421 ccacatgacc cgcgacgacg gctggcgctt tttggcgatt ggccgccatc tggagcggct
     3481 taacctgctg gccgcccagc tgggcctggg cattgccggc tgcacgccgc cgcaggggcc
     3541 ggcgtttgag ctgctgttgg cgctgttcga cagcacgctg acctaccgca gctattacca
     3601 atgccgccag gaactgccgg cgctgctgga cttgctggtg tgcgacgcca acaacccgcg
     3661 cgccatccgc agcgtgcagg cggcgctgcg caacgagctg gaagcgctgc ccaagctgcc
     3721 cggcgcgccg gcgctggccg aatggctgcc cagctcggcg ccggggctgg acgcgctatg
     3781 ccagcgcgcg cccaatcaac agctggaaac cctgcgctgt tttctgcaac acctggacaa
     3841 cgccagcgcg cggctgtccg acgaaatcgg cctgcattac ttcaaccaca acccgtcctg
     3901 gcatcctgac aacccgccgg caaggaacag cccatgctga acaccgccga caccgtgctg
     3961 catatttccc acgtcaccca ctacgactac gccagccgcg tcgatctggc catgcacctg
     4021 ttgcacctgc aaccgctcag ccgcgccgac cagcaactgg aggacttccg gctggacatc
     4081 gacccgccag ccacccgcaa ccaagcctcg ctggattatt ttggcaaccc gcagcaccac
     4141 ctgacgctga ccaccccgca ccacgcgctg accgtgcgcg cagaaagccg cgtgcgccgg
     4201 ctggccgccg ccacgcccaa gcccagcgcc agcccggcct gggaaagcgt gcgcgccgcc
     4261 atgcgctacc gcgccggcca accctggctg ccggcggcgg agttctgttt tccgtccgcc
     4321 tttgtgccgc tgcacccggc gtttcaggcg tacgcccaac tggaattcac ccccggccgg
     4381 ccgctgctgg aagccgccat cggcctgatg gcgcgcatcc accacgaatt cacctacgcc
     4441 accgccagca ccgacctcac caccccggcg ctggaagcct tcagccgccg ccagggcgtc
     4501 tgccaggact tcgcccacat catgatcgcc tgcctgcgca gcctgggcct gcccgcccgc
     4561 tacgtcagcg gctacctgct cacccaaccg ccccccggcc aaccgcgcct attgggcgtg
     4621 gacgccagcc acgcctggct ggcggtgtgg tgcccacaac acgactgggt ggagctcgac
     4681 cccaccaata acctgatcgc cgcgcaaagc cacgccgtca tcgcctgcgg ccgcgactac
     4741 gccgacgtcg ccccgctgcg cggggtgata caaggcggcg gcagccatac cttgtcggtg
     4801 gcggtgagtg tggtgccgga agggga
//
LOCUS       DAOPYX010000228         4802 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_22592_length_4802_cov_8.124289, whole genome shotgun sequence.
ACCESSION   DAOPYX010000228 DAOPYX010000000
VERSION     DAOPYX010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 4802)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4802)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4802
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22592_length_4802_cov_8.124289"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..340)
                     /locus_tag="PKN55_10970"
     CDS             complement(<1..340)
                     /locus_tag="PKN55_10970"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018747400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HNX71808.1"
                     /translation="MSLTLPAQLSLPATSGHTFSLSDSAKPLVLYFYPKDNTPGCTTE
                     SMDFRDAYADFVAAGCQVWGLSRDSIKSHENFKAKLSLPFDLISDPDEIACAAFGVMK
                     LKNMYGKQVRG"
     gene            494..1165
                     /locus_tag="PKN55_10975"
     CDS             494..1165
                     /locus_tag="PKN55_10975"
                     /inference="COORDINATES: protein motif:HMM:NF014460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNX71809.1"
                     /translation="MRRAPVQAALGRQLQQCGLRALPGIACLGAALGLAAVQQMHHVY
                     GANVDFTLRTLLQLLLSELAPLLTALFLLTRSSSGIAAEVAALRVHGEWRTLAAMQVD
                     PLAFVLLPRLLAIPLASALLTLYLLLIAQGAAVLYALDAPDQATFYSMLEPLTPAMAL
                     LCLSKSLLFGALTAASSCLAGLAAEGDWPAVSQASARAVLFGLLAIVAADALFALLPA
                     LWSPA"
     gene            1162..1704
                     /locus_tag="PKN55_10980"
     CDS             1162..1704
                     /locus_tag="PKN55_10980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX71810.1"
                     /translation="MSALTLTLPDGGALALHAGQLTQCAWSPANHAAVSAALLAAGQV
                     MLALPAQGGLLSTLSVADNLTLAASYHGMPWAELDAALALLLPALALDAAAWRTLLAT
                     PTEQLSADDRRRVLLLRAALTPAAAWLIDSDFFADAHPDLAARGQALLAQALPGRLAV
                     WLTRPARAAQPGAEWKEPPP"
     gene            1701..2594
                     /locus_tag="PKN55_10985"
     CDS             1701..2594
                     /locus_tag="PKN55_10985"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HNX71811.1"
                     /translation="MTTLPRPARARRLWWFIALAVLGLAALLAARLWLQGWFEPSTPI
                     RLYAASGSDLKPGMPVKFRAFKIGAVQSITLKDDAHVDVVARIENRYLKFIRQTSLAR
                     LSKEGLIGNSFISIETSDTSAPALGKNAYLALMPGTDMEVIAQQVKDQVDPVIAELHT
                     LLAGLNHPQGPFQQSLRHLAASTGKLNDTHTQLNSLLSHTDTLIDRDARQTLTAINRD
                     LPAVMRQLNATTQATQAASQSLNDAMQASATQLPGLIRDSRLLIQQSNDTLDGLRQHW
                     PLKQLSAPIAPDTGDIHGAQD"
     gene            2578..3396
                     /locus_tag="PKN55_10990"
     CDS             2578..3396
                     /locus_tag="PKN55_10990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX71812.1"
                     /translation="MALRINPRRPHLAAAALLALWLAACAGGPSHPLAQHDAFRQGEL
                     ANRQARAALARGELSSARQLTEQARQHYQRLDARERVIDCELQLATLALRQNDLAGVR
                     AALARLADWQLTPEQQRALAERHISLALREQQAARAADWLKQARAACAAPCARAATLD
                     AFAARIAAARQDLDSARQLAEQALAGARDDSERAAAHRLLAEWTPANAPRTALSHYLA
                     ALEFDQREGYSAGLAQDLAGAAAASQALGDARAAREYRQRAEAVNAAMRAAAGR"
     gene            3598..>4802
                     /locus_tag="PKN55_10995"
     CDS             3598..>4802
                     /locus_tag="PKN55_10995"
                     /inference="COORDINATES: protein motif:HMM:NF024592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4010 domain-containing protein"
                     /protein_id="HNX71813.1"
                     /translation="MLHWLGEVFQLQGTGYAALPQFLTSLGIGLLLGIERERKQDPRA
                     GVRTFALTCLFGTLSGHVAGLAALPLLPIAGLAGIAALTLTAYWHPSACHDSPDPTTQ
                     VALLLAYALGLLVYAGPSELAVALAIIATLLLYLKPELSGLSQKLTRPDILAVLQFGA
                     VSAIILPILPNRAFGPYQAINPYEIWLMVVLISGVSLCGYVAVRLLGEKVGGPVLGLL
                     GGMVSSTATSMSFARQVRDDPKALHLSATVIQLANLVIFVRLAILAALLAPAALGTLL
                     PVLGAALAGGLLATGLGHWRHGSAGGQDEDGFTPHNPVEMRTALVFGGLYAVVTLAAA
                     ALMHQFGSGGLYGVALVSGVTDVDPISLSAFNQFAGGHLNANQVAATLALALLTNSLF
                     KFGMVASLGG"
BASE COUNT          735 a         1653 c         1584 g          830 t
ORIGIN      
        1 tgccgcgcac ctgtttgccg tacatgtttt tcagcttcat caccccgaac gctgcgcagg
       61 cgatttcgtc cgggtcgctg atcagatcaa acggcaggga taatttggcc ttgaagtttt
      121 cgtgcgactt gatcgagtcg cgcgacaggc cccacacctg gcagccggcg gcgacaaaat
      181 cggcgtaagc atcccgaaaa tccatgctct cggtggtgca gccgggggtg ttgtccttgg
      241 ggtaaaaata cagcaccagc ggcttggcgc tgtcggataa gctgaaagtg tgcccgctgg
      301 tggctggcaa gctcagctgg gctggcaggg taaggctcat ggcgactcct gggttagggg
      361 cagggagatg cggcggttgc ccgcagcttt ccatccctca tagaatagcg aaacccgtcc
      421 gctgtcaata ttcatgccac ctgtcatcaa tctgcttgtc caattatgcc atgcgctcgg
      481 ccggctgcca gccttgcgcc gcgcgcccgt gcaggcggcg ctaggacgcc agttgcagca
      541 gtgcggcttg cgcgcgctgc ccggcattgc ctgcctgggc gccgcgctgg gcttggcggc
      601 ggtgcagcaa atgcaccatg tgtacggcgc caatgtggac ttcaccctgc gcaccttgct
      661 gcaattattg ctgtccgagc tggccccgct gttgaccgcg ctgtttttgc ttacccgctc
      721 cagctcgggg attgccgccg aggtggcggc gctgcgcgtg catggcgagt ggcgcacgct
      781 tgccgccatg caagtggacc cgctggcctt tgtgctgctg ccgcgcctct tggccatccc
      841 gctggccagc gcgttgctga ccttgtatct gctgctgatt gcccagggcg cggcggtgct
      901 gtatgcgctg gacgcgccag accaggccac gttttacagc atgctggagc cgctgacccc
      961 ggccatggcg ctgctgtgcc tgagcaaaag cctgctgttt ggcgcgctga ccgccgccag
     1021 cagctgtctg gccggcttgg cggccgaagg cgactggcca gcggtgtcgc aggcgtcggc
     1081 gcgcgcggtg ttgtttggcc tgctggcgat tgtggctgcc gacgcgctgt ttgccctgct
     1141 gccggcgctg tggagcccgg catgagcgcg ctgaccctca ccctgccgga tggcggcgcg
     1201 ctggcgctgc acgccggcca gctgacgcaa tgcgcctgga gcccggccaa tcacgctgcc
     1261 gtcagcgccg ccctgctggc cgccggccag gtgatgctgg cgctgccggc acaaggcggc
     1321 ttgcttagca cgctgagcgt ggccgacaac ctcaccctgg ccgccagcta ccacggcatg
     1381 ccctgggccg agctggacgc cgcgctggcc ctgctgctgc cggcgctggc gctggacgcc
     1441 gccgcctggc gcacgctatt ggccaccccc accgaacaac tcagcgccga cgaccgccgc
     1501 cgggtgctgc tgctgcgcgc ggcgctcacc ccggccgccg cctggctgat cgacagcgat
     1561 ttttttgctg acgcccaccc cgacctggcc gcgcgcggcc aggcgctgct ggcccaagcc
     1621 ctgccgggcc ggctggcggt gtggctcacc cgcccggccc gcgctgcgca gccgggcgct
     1681 gaatggaagg aacccccacc atgaccacgc ttccccgccc ggcgcgcgcg cgccggctat
     1741 ggtggtttat tgcgctggcc gtgcttggcc tggctgcgct gctggccgca cggctgtggc
     1801 tgcaaggctg gtttgagccc agcacgccca tccgcctgta cgccgcctcg ggcagcgacc
     1861 tgaaacccgg catgccggtg aagtttcgcg ccttcaagat cggcgccgtc cagagcatca
     1921 ccctgaaaga cgacgcccat gtggacgtgg tggcgcgcat tgaaaaccgc tacctgaaat
     1981 tcatccgcca gacctcgctg gcccggctga gcaaagaagg gctgattggc aacagcttta
     2041 tcagcattga aaccagcgac accagcgcgc cagcgctggg caaaaacgcc tacctggcgc
     2101 tgatgccggg cacggatatg gaagtgatcg cccagcaagt caaagaccag gtagacccgg
     2161 tgattgccga gctgcacacc ctgctggccg ggctgaacca tccgcaaggg ccgtttcagc
     2221 aaagcctgcg ccatctggcg gcaagcaccg gcaagctgaa cgacacgcac acccagctca
     2281 acagcctgct cagccacacc gacacgctga tcgaccgcga cgcccgccag acgctgaccg
     2341 ccatcaaccg cgacttgccc gcggtgatgc gccagctgaa cgccaccacc caggccaccc
     2401 aggcggccag ccaaagcctg aacgacgcca tgcaggccag cgccacgcaa ctgcccggcc
     2461 tgattcgcga cagccgcttg ctgatccagc aaagcaacga caccctggac ggcttgcgcc
     2521 agcactggcc gctgaaacaa ctgagcgcgc ccatcgcgcc cgacaccgga gacatccatg
     2581 gcgctcagga ttaacccccg tcggccccat ctggccgccg ccgcgctgct ggcgctctgg
     2641 ctggccgcct gcgctggcgg gccatcgcac ccgctggccc agcacgacgc ctttcggcag
     2701 ggcgagctgg ccaaccgcca ggcgcgcgcg gcgctggcgc gcggcgagct gagctcggcg
     2761 cgccagctca ccgaacaagc ccgccagcac tatcaacggc tggacgcgcg cgagcgggtg
     2821 atcgactgcg aattgcagct ggccacgctg gcgctgcggc aaaacgactt ggccggcgtg
     2881 cgcgccgcgc tggcgcggct ggccgactgg cagctgacgc ccgagcaaca acgcgcgctg
     2941 gccgagcggc acatcagcct ggcgctgcgc gaacaacaag cagcccgcgc cgccgactgg
     3001 ctgaagcaag cgcgcgccgc ctgcgccgcg ccatgcgcac gcgccgccac gctggatgcc
     3061 ttcgccgcgc gcatcgccgc cgcccggcaa gacctggaca gcgcccggca actggccgag
     3121 caggcgctgg ccggcgcgcg cgacgacagc gaacgcgccg ccgcccatcg cctattggcc
     3181 gaatggacgc cagccaacgc cccgcgcaca gcgctgagcc actatctggc ggcgctggaa
     3241 ttcgaccagc gcgaaggcta tagcgccggc ctggcgcaag acctggccgg cgccgccgcc
     3301 gccagccagg cgctgggcga cgcgcgcgcc gcccgcgaat accgccagcg cgccgaagcg
     3361 gtgaacgccg ccatgcgcgc cgcggccggg cgctgacaac cttgatgaca tttctgatca
     3421 ggatgcgctg aaaaaatcgt cgcccccgcg aagtcaggaa ttcaaggctt tgatttttct
     3481 gtattttaag cgttgctcgc cacacgcagc gaagcgtgcc tagccagagc cgctacgctg
     3541 agtatgctga gccgctctga ttcaccttat ggatttttac cccggagcac ccatcacatg
     3601 ctgcattggc tgggcgaagt gtttcagttg caaggcaccg gctacgccgc gctgccgcag
     3661 tttttgacca gtctgggcat tggcctgttg ctgggtatcg agcgcgagcg caagcaagac
     3721 ccgcgcgccg gggtgcgcac gtttgcgctg acctgtttat ttggcacgct cagcggccat
     3781 gtggctgggc tggcggcgct gcccttgctg ccgattgccg gcctggccgg cattgccgca
     3841 ctgacgctga ccgcgtattg gcaccccagc gcctgccacg acagcccgga cccgaccacg
     3901 caagtggcgt tgctgctggc gtatgcgctg ggtttattgg tgtacgccgg gccgtccgag
     3961 ctggcggtgg cgctggccat cattgccacc ttattgctgt atctcaagcc ggagttgtcc
     4021 gggctgagcc agaagctcac ccggccggat attctggccg tgctgcaatt tggcgcggtg
     4081 tcggcgatta tcctgccgat tttgcccaat cgcgcctttg gcccgtatca ggcgatcaac
     4141 ccctatgaaa tctggctgat ggtggtgctg atttccgggg tgagcttgtg cggttatgtg
     4201 gcggtgcgcc tgctgggcga gaaagtcggc gggccagtgc tgggcttgct gggcggcatg
     4261 gtgtcgtcca ccgccaccag catgagcttt gcccgccagg tgcgcgacga ccccaaggcg
     4321 ctgcatttgt cagccacggt gatccaactg gccaatctgg tgatttttgt acggctggcg
     4381 attctggccg cgctcttggc cccggcggcg ctgggcacgc tgttgcctgt gctgggcgcg
     4441 gcgctggccg gtgggctgct ggccaccggg ctggggcact ggcggcatgg ctcggctggc
     4501 ggccaggatg aagacggctt taccccgcac aacccggtgg aaatgcgcac cgcgctggtg
     4561 tttggcggcc tgtatgccgt ggtgacgctg gcggcggcgg cgctgatgca ccagtttggc
     4621 tcgggcggtt tgtatggcgt ggcgctggtg tccggggtga ccgatgttga tccgatttcg
     4681 ctgtcggcgt tcaatcagtt tgccggcggc cacctgaacg ccaaccaagt ggccgccacc
     4741 ctggcgctgg ccttgctgac caacagcctg ttcaagtttg gcatggtggc cagcctgggc
     4801 gg
//
LOCUS       DAOPYX010000229         4798 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_22630_length_4798_cov_8.408180, whole genome shotgun sequence.
ACCESSION   DAOPYX010000229 DAOPYX010000000
VERSION     DAOPYX010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 4798)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4798)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4798
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22630_length_4798_cov_8.408180"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1172
                     /locus_tag="PKN55_11000"
     CDS             <1..1172
                     /locus_tag="PKN55_11000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX71814.1"
                     /translation="LSEGWDAANVTHIMGLRAFTSQLLCEQVIGRGLRRVAHDMDENG
                     LFLPEYVNVFGVPLSIFQDVGEEGVAPPPPKPSVRIEVEPNRNQYEIRWPNVLRIESV
                     LKRELVMDWSKVEVLTLDPMNTPLSADVAPSLTSAPNLAMVSEIDLEKATEDFRLQNL
                     IFRAARKLYMQSASSFGGDKQYLAVQLIRLVEQFLASDKLDIPSLWHQDEKRRRLLFA
                     LNMDTVVAHVHRFVTEQNVERLAAVFDESRPIGSTAQMRPWLTTKPCQPTRHSQISHA
                     VHDSVWEKAVVDICEKQKFVAAWAKNDHLDFTVRYLWKGSTRNFVPDYLIRFTNGKTL
                     VLEVKGQDSEQNKAKRAAMQTWVKTINEQGGFGQWCFDVVFDPAKIMDVISSHAQ"
     gene            1704..2906
                     /locus_tag="PKN55_11005"
     CDS             1704..2906
                     /locus_tag="PKN55_11005"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNX71815.1"
                     /translation="MKFPGKWPAWGWRGWGVVVMLLAAAGWWGWRQWSAAAQPGYVTQ
                     TVAVGTLEKVVTALGSVQPKDYVDVGTQVSGQLRELHVQIGDTVQKGQLLAEVDPTVY
                     ATRVAADKASLRDLQAQRARQASVLQLAEVQKKRTRELLALSAASQDAVDMADAAAAQ
                     ARATLESLAAQIDKAQSTLAGDEANLGYTKIYAPMDGVVVSQPVFKGQTVNSAQQAPT
                     LMRVANLDVMTVNAQVAEADVPQLKPGMPVYFTTLGHAERRYQSRIRQILPTPEKVND
                     VVLFTVLIDIDNRARELMTNMTTQTFFVLGRAENLPVVPLSALRPLGEGGKLYGARVL
                     TENGIERRKVKVALSSRTEAAIASGLAAGETLIVSDGRPRAGKAGGGKSNNPSAGARP
                     PGPPGGPM"
     gene            2903..>4798
                     /locus_tag="PKN55_11010"
     CDS             2903..>4798
                     /locus_tag="PKN55_11010"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MacB family efflux pump subunit"
                     /protein_id="HNX71816.1"
                     /translation="MNAPLIELAAVSRCYPNGDSEVRALDEVSLRIAQGEFIALLGPS
                     GCGKSTLMNVLGCLDRPSSGVYRVNGQDVSALDADTLASLRRDTFGFVFQRYNLIATA
                     SATDNVALPAIYAGLPLAARQARARALLAKLGLAERAGHRPGQLSGGQQQRVSIARAL
                     MNDAPVVLADEPTGALDTRSGQEVLALLKQLHGEGRTIILITHDLDIARHAERIVRLR
                     DGRVESDSGPVARAPAPLPARPPSGQPSLRAELGESLSMAFTALRANVFRTVLTLLGV
                     IIGVAAVVAMMAIGDGGKRDVLARISAMGTNLLLVRPGAPGVRPSGELASLTLADAAA
                     LEKVSGVEAVSPERGGSVTLRVGNLDYRTQIKGVWANYPQVRDWPVAEGLFFSKADVD
                     GYAPVIVLGSTVADSLFPDGRSPLGRYVLVKNVPFEVVGVLSSKGATPFGSDQDDIVL
                     IPLTTGFMRVFGKQYASSVTVRVKDVSQIDAVESAVRELLLARHQTEDFQVRNTASIL
                     ETASETQNTLTVLLGSVAAISLLVGGIGVMNIMLVSVTERTREIGVRMATGARMRDIL
                     LQFNIEAIVVCGAGGALGIVLGVLSAWLAASAGVSVAFSPWPALLAFSCAFVTGCVFG
                     YLPARKAA"
BASE COUNT          863 a         1399 c         1654 g          882 t
ORIGIN      
        1 tgctttcgga aggctgggat gccgctaacg tgacgcacat catgggcctg cgcgccttca
       61 ccagccagtt gttgtgcgag caggtgatag gccggggctt gcgccgggtg gcacacgaca
      121 tggacgaaaa cggcttattc ctgccggagt acgtcaatgt gtttggcgtg ccgctgtcga
      181 ttttccagga tgtgggtgaa gaaggcgtgg ccccgccacc gcccaagccc agtgtgcgta
      241 tcgaagtgga gccaaaccgc aaccagtatg aaattcgctg gccgaatgtc ctgcggatcg
      301 aatcggttct aaagcgcgag ctcgtcatgg actggagtaa ggtggaggtg ctcacgctag
      361 acccgatgaa tacaccactc agtgcagacg ttgcgccctc actgaccagc gcaccgaatc
      421 tggcgatggt gtcggaaatc gatcttgaga aagcaacgga agatttccgc ctgcagaacc
      481 tgattttccg cgcagcgcgc aagctataca tgcagagcgc gagcagtttc ggcggcgaca
      541 agcagtacct ggctgtgcaa ctcatccggt tggttgagca gttcttggcg agcgacaagc
      601 tggatattcc cagcctgtgg catcaggacg aaaaacgccg tcgcctgctg tttgctctga
      661 acatggatac ggtcgtggcg catgtgcatc gctttgtgac cgagcagaac gtggaacgac
      721 tggctgccgt gttcgatgag tcgcggccga ttggttcaac cgcacaaatg cgcccatggc
      781 tcaccaccaa gccttgccag ccaacccggc attcgcagat cagccacgct gtgcacgaca
      841 gcgtgtggga aaaggccgtc gtcgatatct gcgagaaaca gaagttcgtt gcggcgtggg
      901 cgaagaacga ccacctggat ttcactgtgc gctacctgtg gaagggctcg acacgtaact
      961 tcgtgcccga ctacctcatt cgcttcacca acggtaaaac gctggtgctg gaagtgaagg
     1021 ggcaggacag cgagcagaac aaggccaaac gtgcggcgat gcagacctgg gtgaagacca
     1081 tcaacgaaca gggcgggttc ggacagtggt gctttgatgt agtgtttgat ccggcgaaga
     1141 tcatggatgt gatttcaagt cacgcccagt agtactccgt ggatgggtgg gggtaaatct
     1201 atagtctact taccccatca cccagaccta caccgcgaca cagaaaagcg gaccaaggca
     1261 tcccctccgt cagcgtcgaa aattgactag tagccagtca ccatgatttg aacagactgg
     1321 cgggtgcttc ggccgcagta cgctgtagac acgcctggat tcccgcctgc gcgggaatga
     1381 cgcggtgtgg tgcggcgagg gggtcttcgt ggcgcatgaa tccgtgtatt tcaacgtgac
     1441 tggcgactag cacatcgttt cattatccaa gcacctgatc caaaacagct ttttccccca
     1501 tcaaaaatcc accaacgttt tccgtggtga acatcctcca ggctgccatc cggatcgacc
     1561 attaatttag cattttttaa cctgccagtt acactacgtt acaaaacccg gcgcgctcga
     1621 caccctgggg acacatggct ctgtttcaat ggtctgatgc gttcaggccc ggcgtgtggc
     1681 cggggcggac aggggaggat ggcgtgaaat ttccaggcaa gtggccggca tggggctggc
     1741 gcgggtgggg tgtggtggtg atgctgttgg cggcggccgg gtggtggggg tggcggcagt
     1801 ggtcggcggc ggcgcagccg ggctatgtca cccagacggt ggcggtgggc acgctggaaa
     1861 aagtggtcac cgcgctgggc agtgtgcagc ccaaggatta tgtggatgtc ggcacccagg
     1921 tgtcggggca gttgcgtgag ctgcatgtgc agattggcga cacggtgcaa aaaggccagt
     1981 tgctggctga ggtggacccc accgtgtacg ccacgcgggt ggcggcggac aaggccagtt
     2041 tgcgcgattt gcaggcgcag cgcgcgcgcc aggcctcggt gttgcagctg gccgaggtgc
     2101 agaaaaaacg cacgcgcgag ttgctggcct tgagcgcggc ttcgcaggat gcggtggaca
     2161 tggccgacgc cgcagcggcg caggcgcgcg ccacgctgga atcgctggcg gcgcagatcg
     2221 acaaggcgca gtccaccctg gccggcgacg aggccaatct gggctacacc aagatttatg
     2281 cgccgatgga tggcgtggtg gtgtcgcagc cggtgttcaa gggccagacg gtgaattcgg
     2341 cgcagcaggc gcccacgctg atgcgggtgg ccaatctgga tgtgatgacg gtcaacgccc
     2401 aggtggccga ggctgatgtg ccgcagctca agccgggcat gccggtgtat ttcaccacgc
     2461 tgggccacgc cgagcggcgc tatcaaagcc gcattcgcca gattttgccc accccggaaa
     2521 aagtcaacga tgtggtgttg ttcaccgtgc tgatcgacat cgacaaccgc gcgcgcgagc
     2581 tgatgaccaa tatgaccacg caaacctttt ttgtgctggg ccgcgcggaa aatctgccgg
     2641 tggtgccgct gtcggcgctg cggccgctgg gcgagggcgg caagctgtat ggcgcgcggg
     2701 tgctcaccga aaacggcatt gagcggcgca aagtcaaggt ggcgttaagc agccgcaccg
     2761 aggcggcgat tgccagcggc ctggcggcgg gcgagacgct gattgtcagc gatggccggc
     2821 cgcgcgcggg caaggccggc ggcggcaaga gcaataaccc cagcgcgggc gcgcgtccgc
     2881 ccggcccgcc aggaggcccg atgtgaacgc gcccctgatc gaactggcgg cggtgtcgcg
     2941 ctgctacccc aatggcgaca gcgaggtgcg cgcgctggat gaagtcagcc tgcgcattgc
     3001 ccagggcgag tttattgccc tgctcgggcc gtccggctgc ggcaagtcca cgctgatgaa
     3061 tgtgctgggc tgcctggatc ggcccagcag cggggtgtat cgggtgaatg gccaggatgt
     3121 cagcgcgctg gatgccgaca cgctggccag cctgcgccgc gacacgtttg gctttgtgtt
     3181 tcagcgctat aacctgatcg ctaccgccag cgccaccgac aatgtcgccc tgccggcgat
     3241 ttatgccggc ctgcccttgg cggcgcgcca ggcgcgcgca cgggcgctat tggccaagct
     3301 gggtctggcc gaacgcgccg gccatcggcc cggacagttg tcaggtggcc agcagcaacg
     3361 ggtgtcgatt gcccgcgcgc tgatgaacga cgcgcccgtg gtgctggccg acgagcctac
     3421 cggcgcgctg gacacccgca gcggccagga ggtgctggcg ctgctcaagc agctgcacgg
     3481 cgagggccgc accattattt tgattaccca cgatctggac atcgcccgcc acgccgagcg
     3541 cattgtgcgc ctgcgcgatg gccgggtgga gtccgacagc ggcccggtgg cgcgcgcgcc
     3601 tgcgccgctg ccggcgcggc cgccctccgg ccagcccagc ttgcgcgccg agctgggcga
     3661 atcgctgagc atggcgttta ccgccttgcg cgccaatgtg tttcgcaccg tgctgacctt
     3721 gttgggggtg attatcggcg tggcggcggt ggtggcgatg atggcgattg gcgacggcgg
     3781 caagcgcgat gtgctggcgc ggatttccgc catgggcacc aatctgctgc tggtgcgccc
     3841 cggcgcgccc ggcgtgcgcc ccagcggcga gctggcctcg ctgaccctgg ccgacgccgc
     3901 cgcgctggaa aaagtcagcg gcgtggaggc ggtgtcgccc gagcgcggcg gcagcgtcac
     3961 cttgcgggtg ggcaatctgg attaccgcac gcaaatcaag ggcgtgtggg ccaattatcc
     4021 acaggtgcgc gattggccgg tggcggaggg gctgtttttc agcaaagcgg acgtggatgg
     4081 ctacgcgcca gtgatagtgt tgggcagcac ggtggcggac agtttattcc ccgatggccg
     4141 ttcgccgctg ggccgctatg tgctggtgaa aaacgtgcca tttgaagtgg tgggcgtgct
     4201 cagcagcaag ggcgccacgc cgtttggcag cgaccaggac gatattgtgc tgattccgct
     4261 gaccaccggc tttatgcgcg tgtttggcaa gcagtacgcc agctcggtga cggtgcgggt
     4321 gaaagacgtc agccagatcg acgccgtcga gtccgccgtg cgcgagctgc tgctggcgcg
     4381 ccaccagacc gaggattttc aggtgcgcaa caccgcatcg attctggaaa ccgccagcga
     4441 aacgcaaaac accctgaccg tgctgctggg ctcggtggcg gcgatttcgc tgctggtggg
     4501 cggcattggc gtgatgaaca tcatgctggt gtcggtgacc gaacgcaccc gcgaaatcgg
     4561 cgtgcgcatg gccacgggcg cgcgcatgcg cgacattttg ctgcaattca atatcgaagc
     4621 cattgtggtg tgcggcgctg gcggcgcgct gggcattgtg ctgggcgtgc tcagcgcctg
     4681 gctggccgcc agcgccgggg tgagcgtggc gttttcgccc tggccggcgc tgctggcctt
     4741 tagctgcgcc tttgtcaccg gctgcgtgtt tggctacctg ccggcgcgca aggcggcc
//
LOCUS       DAOPYX010000230         4746 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_23000_length_4746_cov_13.047538, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000230 DAOPYX010000000
VERSION     DAOPYX010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 4746)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4746)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4746
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23000_length_4746_cov_13.047538"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1103)
                     /locus_tag="PKN55_11015"
     CDS             complement(<1..1103)
                     /locus_tag="PKN55_11015"
                     /inference="COORDINATES: protein motif:HMM:NF024905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HNX71817.1"
                     /translation="MSARHLWRQVRQQLYAEGRQLITIRPSDRPWQMPFAAALASGLP
                     LLVGAAFGRVDYGLVSSLGGLVFLYLPDTPLHHRMVSLMASAFAMIACYTLGIISHLL
                     PAGMLLALATVAMLSNMTCRFYQVNPPGSLFFFMAAAIGAYSPVELLQAPFKVGLLAL
                     GCLLACVVAFVYSLFILSRRLPRPVQPLPPASFEFVVFDSVLIGVCVGVSLLLAQALQ
                     LHNPYWVPVSCLAVIQGMSLRAVWEKQLQRLLGTSIGLLLSWGLLALPLDPWRLALMI
                     IVLTFIVETAVVRHYGFAAIFITPLTLLLAEAVTLGHGSVSALIQARFVDTLLGCLVG
                     LVGGVCLHSPRFRAWASPPLRRLLTPQRPRQAA"
     gene            complement(1190..1972)
                     /locus_tag="PKN55_11020"
     CDS             complement(1190..1972)
                     /locus_tag="PKN55_11020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018150932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HNX71818.1"
                     /translation="MPTPRMPVQRVNVPKLSDAIMHQLESMILEGSLKPGDRLPPERQ
                     LAEQLGVSRPSLREAIQRLAARGMLVSRQGGGTYVTDQLAAAFSDPWQQLLVEHPNLH
                     RDVLEFRRTLEGAMAECAARRATEPDRERLAALMDELEAAYAGHDLLTLSRMDVAFHQ
                     AIADAAHNVLFAHLSSSLLSMLKTHVRDNIANLFDVAPVSGQLLAQHRAIWQAIHERN
                     PQAARAAAEAHIDFVDQTLNVIRVEHEREERAMRRAQAVEKG"
     gene            2266..3027
                     /locus_tag="PKN55_11025"
     CDS             2266..3027
                     /locus_tag="PKN55_11025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012698286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HNX71819.1"
                     /translation="MHATPAPARPGDVYFFGTCLLDLFLPEAGLDAMTLLEREGVRVH
                     FPQAQSCCGQPAFTSGQPGEAREVARSQLGLFDQPWPIVVPSGSCGGMIKHHWPTLFA
                     GDADEARACAVAGRVVEFADFLLNVLRIELRDQGKPTKVAVHTSCSARREMNTHVHGW
                     ALVDQLAQVERVVHDHESECCGFGGVFSLRHPDISGAMVSDKSAALQDSGAVEFVTAD
                     GGCLLNINGKLAHDGKAFRGKHLASFLLERTGGKA"
     gene            3024..3728
                     /locus_tag="PKN55_11030"
     CDS             3024..3728
                     /locus_tag="PKN55_11030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004282792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein"
                     /protein_id="HNX71820.1"
                     /translation="MSARDRILGKLRAAAPAQTPVEPDVAGYYARHTLQHALPARLAH
                     FSHMMRAVHTEVIYTRADSWPQALAARAAQAGWRHVLLAPNTAHGARAAQALQGLATP
                     PQLKSYDRPIDDWKAELFHDVDAAFTTTRAGIAETGTLIVWPDADEPRAMSLVPPVHI
                     ALFDASRLYENFYQAVSTEGWANGLPTNSLLISGPSKTADIQQTLAYGAHGPRELIVL
                     AIVPDDIDLAELEAAQ"
     gene            3725..>4746
                     /locus_tag="PKN55_11035"
     CDS             3725..>4746
                     /locus_tag="PKN55_11035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004282793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein B"
                     /protein_id="HNX71821.1"
                     /translation="MSTHPITVHPARSFKANSHDAVNDPHLRKSFRGAMDFLMAKRAA
                     QFPDHNELEALRSLGETVRQRCLAKLPQLLETLEAQLTANGVKVHWAETPEEANAIIH
                     GLIAARNGKLAVKGKSMVSEETELNHYLEAHGVQAVESDMGEYIVQLAGEKPSHIIMP
                     AIHKTKAQIATLFHDNIPAAPYTEDVDELIQLGRNALRGMYLKADVGISGVNFAVAET
                     GTLCLVENEGNGRMCTTVPDMHIAITGIEKVVEKLSDVIPLYSILPRSATGQAITTYF
                     NMISRPRQPGEKDGPREVHLVLLDNGRSQAYADEQLRKTLQCIRCGACMNHCPVYSRI
                     GGHAYGT"
BASE COUNT          952 a         1531 c         1489 g          774 t
ORIGIN      
        1 tctgcggcct ggcgtggccg ttgcggcgtc agcaggcgtc gcaacggcgg gctggcccag
       61 gcgcgaaagc gtgggctatg caggcagacg ccgccgacca ggcccaccag acagcccagc
      121 agggtatcga caaagcgcgc ctggatcagc gcgctgactg agccatgccc cagcgtcacc
      181 gcttcggcca gcagcaaggt cagcggggtg atgaagatgg cggcaaagcc atagtggcgc
      241 accacggcgg tttcgacgat aaaggtcagc acaatgatca tcagcgccag ccgccaaggg
      301 tcgagcggca gcgccagcag cccccaggac agcaagagcc cgatgctggt gcccagcagc
      361 cgttgcagct gtttttccca caccgcgcgt agcgacatgc cctgaatcac cgccagacag
      421 ctcaccggca cccagtacgg gttatgcagc tgcaaggcct gcgccagcag cagcgacacc
      481 cccacacaca cgccgatcag caccgaatcg aataccacaa actcaaagct ggctggcggc
      541 aaaggctgca ccggccgtgg caggcggcgc gacagaataa acaggctgta gacaaaagcc
      601 accacgcagg ccagcagaca gcccagcgcc aacaagccca ccttgaatgg tgcttgcagc
      661 agctcgaccg gagaatacgc gccaatcgcc gccgccatga aaaagaacag gctgccaggc
      721 gggttcacct ggtaaaaccg gcaggtcata ttgctcagca tggccaccgt ggccagcgcc
      781 agcagcatgc cggccggcag caggtggctg atgatcccca aggtgtagca ggcgatcatg
      841 gcaaaggcac tggccatcag cgacaccatg cgatggtgca gcggggtatc cggcagatac
      901 aaaaacacca agccacccag cgaggacacc aggccataat ccacccgacc aaacgctgcc
      961 cccaccagca agggcaaacc agacgccagc gccgcggcaa acggcatctg ccaggggcgg
     1021 tcgctggggc ggatggtgat caattgccgc ccctcggcat acagttgctg acgcacctgg
     1081 cgccacaagt gacgagcaga catcagcgtg aactgtccct gtggatgagc tggcgcggcg
     1141 cggcaacatg cctttggctt ggtttatgtg tggtggcttg catttggtct taccctttct
     1201 ccaccgcttg cgcccggcgc atggcgcgtt cttcgcgttc gtgctccacc cggatcacgt
     1261 tcagcgtctg atcgacaaaa tcaatatgtg cttcggcggc ggcgcgggcg gcttgcgggt
     1321 tgcgttcgtg gatggcctgc cagatggcgc ggtgctgggc caggagctgg cccgacaccg
     1381 gggcgacgtc aaacagattg gcgatattgt cgcgcacatg ggttttcagc atcgacagca
     1441 ggctggacga caggtgggcg aacagcacat tgtgcgcggc gtcggcgatg gcctggtgaa
     1501 aggccacgtc catgcgcgac agcgtcagca ggtcgtggcc ggcgtaggcg gcttccagct
     1561 cgtccatcag cgccgccagg cgttcgcggt cgggttcggt ggcgcggcgc gcggcgcatt
     1621 cggccatggc gccttccagc gtgcggcgaa actccagcac atcgcggtgc aagttggggt
     1681 gctccaccag cagttgctgc caggggtcag aaaacgccgc cgccagttga tcagtgacat
     1741 aggtgccgcc gccctggcgg ctgaccagca tgccgcgcgc ggccaggcgc tggatggctt
     1801 cgcgcagcga ggggcgcgac acgcccagtt gttcggccag ctggcgctcg ggcggcaggc
     1861 ggtcgccggg tttgagcgag ccttccagaa tcatgctttc cagctggtgc atgatggcgt
     1921 cggacagctt gggcacattg acgcgttgaa caggcattct gggcgtgggc atgtcgtctt
     1981 ctccttgcaa cagattttcg ttttgcgcca aaaccggggg ataaacgcaa aacgaacgct
     2041 gttttttcgt ttttgttcga atattccagg ttttttccct gaaatcatca agagcgcaca
     2101 gtggctgcgc gcaaaagaag ttattggtct taccaatgat acgcccttac cttcacctag
     2161 aaaagggctt aaattaaact tcgttgacaa cggaagtctt tgattttaca ttcactgaac
     2221 acgaaaaata ttggtcttac caattgacca agagggagac aacccatgca cgccaccccc
     2281 gcgcccgccc gccccggcga tgtgtatttt tttggcacct gtttgcttga tttgttcttg
     2341 cccgaagccg ggctggacgc catgacgctg ttggagcgtg aaggcgtgcg cgtgcatttt
     2401 ccgcaggcgc aaagctgctg tggccaaccc gccttcacca gcggccagcc cggcgaagcg
     2461 cgcgaggtgg cgcgcagcca gctggggctg tttgatcagc cgtggccgat tgtggtgccg
     2521 tccggttcgt gtggcggcat gatcaaacac cactggccga cgctgtttgc cggcgacgcc
     2581 gacgaggcgc gcgcctgtgc cgtggccggt cgggtggtgg aatttgccga ttttctgctg
     2641 aatgtgctgc gcattgaact gcgcgaccag ggcaagccca ccaaggtggc ggtgcacacc
     2701 tcgtgttcgg cgcggcgcga gatgaacacc catgtgcacg gctgggcgct ggtggatcag
     2761 ctggcccagg tcgagcgcgt ggtgcacgac cacgaatccg aatgctgtgg ttttggcggg
     2821 gtgttttcgc tgcgccaccc ggatatttcc ggggcgatgg tcagcgacaa gagcgcggcg
     2881 ctgcaagaca gcggcgcggt ggagttcgtc accgccgacg gcggctgttt attgaatatc
     2941 aacggcaagc tggcccacga tggcaaagcc tttcgcggca agcatctggc cagttttctg
     3001 ctggaacgca ccggaggcaa ggcatgagcg cgcgcgaccg cattctgggc aagctgcgcg
     3061 ccgccgcccc ggcgcaaacc ccagtcgagc ccgatgtggc cggctactac gcccgccaca
     3121 ccctgcaaca cgcgctgccg gcgcgactgg cgcactttag ccacatgatg cgcgcggtgc
     3181 acaccgaggt gatctacacc cgcgccgaca gctggccgca ggcgctggcc gcccgcgccg
     3241 cccaggctgg ctggcgtcat gtgctgctgg cccccaacac cgcccacggc gcgcgcgccg
     3301 cccaggcgct gcaaggcctg gccacgccgc cgcagctgaa aagctacgac cggcccatcg
     3361 acgactggaa ggccgagctg tttcatgacg tggacgccgc cttcaccacc acgcgcgccg
     3421 gcattgccga aaccggcacg ctgattgtct ggccagacgc cgacgagccg cgcgccatga
     3481 gcctggtgcc gccggtgcac atcgccctgt ttgacgccag ccgcctgtac gaaaactttt
     3541 atcaagccgt cagcacggaa ggctgggcca acggcctgcc gaccaacagc ctgctgattt
     3601 ccgggccgtc caaaaccgcc gacatccagc aaacgctggc ctacggcgcg catggcccgc
     3661 gtgaactgat tgtgctggcc atcgtgccgg acgacatcga ccttgccgaa ctggaggccg
     3721 cccaatgagc acgcatccga ttaccgtgca cccggcgcgc agcttcaagg ccaatagcca
     3781 cgatgcggtg aacgacccgc acctgcgcaa gagctttcgc ggggcgatgg acttcctgat
     3841 ggccaagcgc gcggcgcagt ttcccgacca caacgagctg gaagcgctgc gcagcctggg
     3901 cgaaaccgtg cgccagcgct gcctggccaa gctgccgcag ctgctggaaa ccctggaagc
     3961 ccagctcacc gccaatggcg taaaagtgca ctgggcggaa accccggaag aagccaacgc
     4021 catcatccat ggcctgatcg ccgcccgcaa cggcaagctg gcggtcaagg gcaagtcgat
     4081 ggtcagtgaa gaaaccgagc tgaaccacta cctggaagcg cacggcgtgc aggcggtgga
     4141 aagcgatatg ggcgagtaca tcgtccagct ggcgggcgaa aagcccagcc acatcatcat
     4201 gccggcgatt cacaaaacca aggcgcaaat cgccacgctg tttcacgaca atatcccggc
     4261 cgcgccgtac accgaagacg tggacgagct gatccagctg ggccgcaatg ccctgcgcgg
     4321 catgtatctg aaggccgatg tcgggatttc cggggtgaac tttgccgtgg cggaaaccgg
     4381 cacgctgtgc ctggtggaaa acgaaggcaa cggccgcatg tgcaccaccg tgccggacat
     4441 gcacatcgcc atcaccggca ttgaaaaagt ggtggaaaaa ctcagcgacg tgattccgct
     4501 gtattccatc ctgccgcgct cggccaccgg ccaggccatc accacctatt tcaatatgat
     4561 ctcgcgcccg cgccagcccg gcgaaaaaga cggcccgcgc gaggtgcatc tggtgctgct
     4621 ggacaacggc cgctcgcaag cctacgccga cgaacaactg cgcaaaaccc tgcaatgcat
     4681 ccgctgcggc gcctgcatga accattgccc ggtgtacagc cgcattggcg gccacgccta
     4741 cggcac
//
LOCUS       DAOPYX010000231         4679 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_23435_length_4679_cov_13.291090, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000231 DAOPYX010000000
VERSION     DAOPYX010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 4679)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4679)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4679
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23435_length_4679_cov_13.291090"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(115..1314)
                     /locus_tag="PKN55_11040"
     CDS             complement(115..1314)
                     /locus_tag="PKN55_11040"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNX71822.1"
                     /translation="MHPTPPAPRIAIVVDWLQDYAGAERVLEQLLLTFPQAEVFAVVD
                     FVPEASRGFLQGKPVHTTFIQRLPLARRLFRQYLPLMPLAVEQLDLSGFDIVISSSHA
                     VAKGVLTGPDQLHVSYVHSPLRYAWDMQHEYLREAGMARGLKSLLTRWLLHRLRLWDV
                     RTANGVDQFVANSAFIARRVRKAYRREAEVIYPPVDLSAFIPSAAARGQAYVTVSRMV
                     PYKRVDLIVEAFAGMPDKQLIVIGDGPEMAKVRAKAGSNVQILGHLPFEAMREHVQRA
                     RAFVFAAQEDFGIAPVEAQACGTPVIAYGRGGAAETVRGLQQNPPTGVLFEEQTVEAL
                     REAVRQFEQHAARITPAACRQNAERFAIGVFRQRMAALVVSRWSGLQGEVKSGVTGFK
                     PIQPKAL"
     gene            complement(1346..2776)
                     /locus_tag="PKN55_11045"
     CDS             complement(1346..2776)
                     /locus_tag="PKN55_11045"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNX71823.1"
                     /translation="MTISSAPLADPAPPPWSPVLLFSLCLSMLLSALGSSLGNVALPA
                     LAQAWRAPFALVQWVALGYLLTLTALSVSVGRLSDSLGRRRLLLAGIAVFSIGSALCA
                     AAPSLPMLIAARALQGLGAACMMALSMAGVSDWAPAGRAGRAMGWLGSLSAVGSTLGP
                     SLGGGLIAWSGWPAMFWLSAALGALTWLLSWRTLPADRPRHGPAAPFDGQGALWLALA
                     LVCYALAGAAGFTLSGLAWLAAMLLATAGLVWRQGRAPAPLIPLPLLRHPALRAGLLG
                     SLLVSTVMMATLVVGPFYLTFALGLTPGKLGLAVALGPLAAALSSAPAGRLVDRFGAP
                     RVAGGGLCGLAVGAALLALLPLSSGLAGYLLPIVSMTVSYALFQTANNTAVMQCAAPQ
                     QRGVLAGLLNVSRNLGLTSGAALMGALFAWGVGNSDMAHAGPEAVAAGMRLTFAVAVL
                     MVGLAGWRSMMGRWPDAGSQTPANTR"
     gene            2985..3899
                     /locus_tag="PKN55_11050"
     CDS             2985..3899
                     /locus_tag="PKN55_11050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HNX71824.1"
                     /translation="MCYRILMNLPDLNLLITLDAVLSEGSVAGAARRLGLSPSAMSRA
                     LARLREVTGDPLLVRAGRGLTPTPRAVLLREQVGPVLLAAQQLLQPAAALDVAGLART
                     FTLRVSDGFVDNFGPALLAHVAAQAPAVRLSFVQKIDRDSQALRSGAVDLETGVIGAD
                     SAPELRVQALFRDQFIGVARADHPLIGQLHTLEGYCQGSHVALARQGMPHGPVDVALQ
                     ALGRQRRIVAQVSGFATALALARASELIASVPARHTGQLRDGMHSFALPFAAPTFTVS
                     LMWHPRMEGDAAHRWLRGCVRAVCAASL"
     gene            3934..>4679
                     /locus_tag="PKN55_11055"
     CDS             3934..>4679
                     /locus_tag="PKN55_11055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011133821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HNX71825.1"
                     /translation="MSLLPHQLELLSPAKTTDIGREAILHGADAVYIGGPGFGARHNA
                     GNSVSEIAQLVEFAHRYHARIFVTLNTILHDDELEPARQLIHQFYDAGVDALIIQDMG
                     IMELDIPPIQLHASTQCDIRSADKARFLADAGLSQLVLARELSLPDIRQVAARVGDAA
                     TIEYFIHGALCVAFSGQCYISHAETGRSANRGDCSQACRLPYTLTDEQGRVVAYDKHL
                     LSMKDNNQSDNLEALIDAGVRSFKIEGRYK"
BASE COUNT          839 a         1599 c         1539 g          702 t
ORIGIN      
        1 gatgtcagcg aagggcaagc gccagcgctg ggccgtggca gaaaaaccac atcgggataa
       61 taagccgcgc cggcctcacc gctggcgtgc cgtcaccaca cgccagccct cccgctacaa
      121 ggcctttggc tgaatcggct tgaacccggt caccccggac ttcacctcgc cctgcaaacc
      181 cgaccagcgc gacaccacca gcgccgccat gcgctgacgg aacacgccaa tggcaaagcg
      241 ctcggcgttt tgccggcacg ccgccggggt gatgcgcgcc gcgtgctgct caaactgccg
      301 caccgcctcg cgcagcgcct cgacggtttg ttcttcaaac aacaccccgg tcggcgggtt
      361 ctgctgcaaa ccgcgcactg tctcggccgc gccgccgcgc ccgtaggcaa tcaccggcgt
      421 gccgcaagcc tgcgcctcga ccggggcgat gccaaaatcc tcctgcgcgg caaacacaaa
      481 ggcgcgggcg cgctgcacat gctcgcgcat cgcctcaaac ggcaaatgcc ccaaaatctg
      541 cacattgctg ccggccttgg cgcgcacctt ggccatctcc ggcccgtcgc caatcacaat
      601 cagctgcttg tccggcatgc cggcaaacgc ctcgacaatc aaatccaccc gcttatacgg
      661 caccatgcgc gacaccgtca catacgcctg gccgcgcgcc gccgccgacg ggataaaggc
      721 gctcaggtcc actggcggat aaatcacctc ggcctcgcgc cgatacgcct tgcgcacccg
      781 ccgggcgata aacgccgaat tggcgacaaa ctgatccacc ccattggcgg tgcgcacatc
      841 ccacagccgc agccggtgca atagccagcg cgtcagcagg cttttcagcc cgcgcgccat
      901 gcccgcctcg cgcaggtatt cgtgctgcat gtcccaggcg tagcgcagcg gcgaatgcac
      961 atagctgaca tgcaactgat ccggcccggt cagcaccccc ttggccaccg catggctgct
     1021 ggaaatcaca atgtcaaaac cgctcaaatc cagctgctcg accgccagcg gcatcaaggg
     1081 cagatactgg cgaaacagcc gccgcgccag cggcaggcgc tggataaagg tggtgtgcac
     1141 cggcttgccc tgcaaaaacc cgcgcgacgc ctccggcaca aaatccacca cggcaaacac
     1201 ctcggcctgc gggaaggtca gcagcaactg ctccagcacc cgctcggcgc cggcgtagtc
     1261 ctgcaaccag tcaacaacaa tggcaatgcg cggggcgggc ggtgtggggt gcatggcgga
     1321 tcctgaatga atggcggaat ggggattatc gcgtgtttgc cggcgtttgg ctgcctgcat
     1381 ccggccagcg gcccatcatg ctgcgccacc cggccagccc caccatcaat accgccacgg
     1441 caaacgtcag ccgcatcccc gccgccaccg cctcgggccc ggcgtgggcc atgtcactat
     1501 tgcccacgcc ccaggcgaat agcgcgccca tcagcgctgc gccgctggtc aggcccagat
     1561 tgcgcgagac attcaacagc ccggccagca ccccgcgctg ctggggggcg gcgcattgca
     1621 tcaccgcggt gttattggcg gtctggaaca gcgcgtaact gaccgtcatg ctgacgatgg
     1681 gcagcagata gccggccagg ccgctgctca gcggcaggag cgccagcagg gcggcgccca
     1741 cggccaggcc acacaggccg ccgccggcca cacgcggcgc gccaaagcgg tccaccagac
     1801 gaccggcggg cgcgctgctc aacgcggcgg ccagcgggcc aagcgccact gccaggccca
     1861 gcttgcccgg cgtcagcccc agggcaaagg tcagataaaa cggcccgacc accagggtgg
     1921 ccatcatcac cgtggacacc agcaggctgc cgagcaagcc agcgcgcaag gcggggtggc
     1981 gcaatagcgg cagcggaatc agcggcgcgg gcgcgcggcc ttgccgccag accaggcccg
     2041 ccgtcgccaa cagcatggcc gccagccagg ccaggccgct gagggtgaag ccggcggcgc
     2101 cggccagcgc atagcacacc agggccagcg ccagccacag cgcgccctgg ccatcaaacg
     2161 gcgcagccgg cccgtggcgc ggtcggtcgg cgggcagggt gcgccagctc agcagccagg
     2221 tcagcgcgcc caatgccgcg ctaagccaga acatcgccgg ccagcccgac caggcaatca
     2281 gcccgccacc cagcgacggc cccagcgtgg agcccaccgc cgacaagctg cccagccaac
     2341 ccatggcgcg gccggcgcgc cctgccggcg cccaatcgct gaccccggcc atgctcagcg
     2401 ccatcatgca cgccgcgccc agcccttgca gcgcccgcgc ggcaatcagc atcggcaagc
     2461 tgggcgcggc ggcgcacagc gccgagccaa tgctgaacac agcgatgccg gccaagagca
     2521 aacgtcggcg gcccaggctg tcgctcaagc gcccgacgct gacgctgagc gcagtaaggg
     2581 tgagcagata gcccagcgcc acccattgca ccagggcaaa cggcgcgcgc caggcttgcg
     2641 ccagcgcggg cagggccaca ttgcccaggc tggagcccag cgcggacagc agcatggaca
     2701 aacacaggct gaacaacagc acgggcgacc acggcggcgg ggcggggtca gcgaggggcg
     2761 cggaggatat cgtcatggtg tgctttcggt gggtgatagg agcggctcac aaaacccagc
     2821 gcagcggtcc tggccaggcg tgcgcccgca gacacggcaa ggtcgcgcaa gcacgccagg
     2881 aggcttgtgt gagcggctct cagtcaggcc agtctaggca gcgggctgaa caggcggaag
     2941 acgcatggtt tgcaaggtat tgttgcgtgt ggcgcgaggt gttgatgtgc taccgtatcc
     3001 tgatgaacct tccggatttg aatttattga tcaccctgga tgccgtgctc agtgagggca
     3061 gtgtggcggg ggcggcgcgg cgtttgggct tgagtccgtc ggcgatgagc cgggccttgg
     3121 cgcgtttgcg cgaggtgacc ggcgaccctt tattggtgcg cgccgggcgg ggcttgacgc
     3181 ccacgccgcg cgccgtgctg ttgcgcgagc aggtggggcc ggtgctgctg gcggcgcagc
     3241 agctgttgca gccggcggct gccctggatg tggccgggct ggcgcgcacg tttaccctgc
     3301 gggtcagcga tggctttgtg gataactttg gcccggcctt gctggcgcat gtcgcggcgc
     3361 aagcgccagc ggtgcgcttg agttttgtgc aaaaaatcga ccgcgacagc caggctttgc
     3421 gcagcggggc ggtagacctg gaaaccgggg tgattggcgc ggattcggcg ccggaactgc
     3481 gtgtgcaggc cttgtttcgt gatcagttta ttggcgtggc gcgggcggat caccccttga
     3541 tagggcagtt gcacaccttg gagggctatt gtcagggcag ccatgtggcg ctggcgcgcc
     3601 agggcatgcc gcatgggccg gtggatgtgg ctttgcaggc gctgggccgg cagcggcgga
     3661 ttgtcgccca ggtcagcggt tttgccactg cgctggcgct ggcgcgcgcc agtgagctga
     3721 ttgccagcgt gccggcgcgc cacaccggcc agttgcgcga cggcatgcac agctttgccc
     3781 tgccgtttgc cgcgccgacg tttactgtgt cgctgatgtg gcatccgcgc atggaaggcg
     3841 acgcggcgca tcgctggttg cgcggatgcg tgcgggcggt gtgcgcggct tcgttataat
     3901 ccggtttctt tttactcccc caggtaagcc cccatgagtc tgttgccgca tcaactggaa
     3961 ttgctgtccc cggccaaaac caccgacatt ggccgcgagg ccatcctgca cggggcggat
     4021 gcggtgtaca tcggcgggcc ggggtttggc gcgcggcaca atgccggcaa ttcagtcagc
     4081 gagattgccc agctggtgga atttgcccac cgctaccacg cgcggatttt tgtcaccctc
     4141 aacaccattc tgcacgacga cgagctggag ccggcgcggc agctgattca ccagttttac
     4201 gatgccggcg tggacgcgct gattattcaa gacatgggca tcatggagct ggacatcccg
     4261 cccattcagc tgcacgcctc cacccagtgc gatatccgca gcgccgacaa agcgcgtttt
     4321 ttggccgacg ctggcttgtc gcagctggtg ctggcgcgcg aattgtcgct gccggacatc
     4381 cgccaggtgg ccgcgcgggt gggcgacgcc gccaccattg aatatttcat ccacggcgcg
     4441 ctgtgcgtgg cgttttccgg gcagtgctat atcagccatg ccgaaaccgg gcgcagcgcc
     4501 aatcgcggcg actgctcgca agcctgtcgc ctgccgtaca cgctgaccga cgaacaaggc
     4561 cgggtggtgg cctacgacaa gcatttgctg tcgatgaagg acaataacca aagcgacaat
     4621 ctggaagcgc tgatcgacgc tggcgtgcgc tcgttcaaga tcgaaggccg ctacaagga
//
LOCUS       DAOPYX010000232         4677 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_23452_length_4677_cov_7.826915, whole genome shotgun sequence.
ACCESSION   DAOPYX010000232 DAOPYX010000000
VERSION     DAOPYX010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 4677)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4677)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4677
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23452_length_4677_cov_7.826915"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(62..631)
                     /locus_tag="PKN55_11060"
     CDS             complement(62..631)
                     /locus_tag="PKN55_11060"
                     /inference="COORDINATES: protein motif:HMM:NF016819.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HupE/UreJ family protein"
                     /protein_id="HNX71826.1"
                     /translation="MKLRNALALAAVLAAPLAHAHTGAHSHFDLFAGLSHPLSGADHL
                     LAMLAVGIWGAMLGGAARLALPASFVLAMLLGGVAGHLGMPLPAIETGIALSVLALGL
                     LVSLSVRMPTVLAMALTAGFAVFHGFAHGVEAPADGSFWLYGAGFAVATAALHAAGFT
                     LNQHLTSTARRVLGAGMAATGGLLLSGML"
     gene            complement(893..1123)
                     /locus_tag="PKN55_11065"
     CDS             complement(893..1123)
                     /locus_tag="PKN55_11065"
                     /inference="COORDINATES: protein motif:HMM:NF027525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="band-7 C-terminal domain-containing protein"
                     /protein_id="HNX71827.1"
                     /translation="MQANAEAIASVAHALEQQGGDRAAALKVAEQYVAAFGQIARQGT
                     TVVIPSNLADLAGLVQGALSVLPKKSADSSQG"
     gene            1384..1659
                     /locus_tag="PKN55_11070"
     CDS             1384..1659
                     /locus_tag="PKN55_11070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX71828.1"
                     /translation="MSQSVLDTMADATMAVLAWLGLNTEYWPPVLGVALFVWVLSALW
                     SLYRNLGGVDASGTSSDWDMSDTSDSASSSDCGDSGGGGCDCGGGDD"
     gene            1729..3177
                     /locus_tag="PKN55_11075"
     CDS             1729..3177
                     /locus_tag="PKN55_11075"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNX71829.1"
                     /translation="MSTTLPVVLIVDDEIRSLETLRRTLEEAFEVFTAASTDEALAIL
                     QNEFIHVVISDQRMPDMTGVAFLKLVRERWPEVARIILSGYADNADIIAAINEAGIYQ
                     YLLKPWRPDALLLTVQGAARLATLQRENELLNVELKAGEPWMRSRVAAKRQQASDAFA
                     MHSIVRAANSPLNRICALLTKVAVHDIAVLIEGESGVGKELLARALHYASHRAQRPFV
                     GENCAALPEQLLESVLFGHKRGAFTGAYQDHVGLFQQADGGTIFLDEIGETSPAFQAK
                     LLRVLQEGEIRPVGSSRSLRVDVRVVSATNRTLADLVQQGRFREDLYYRLSPFAVLTP
                     PLRERPEDIPLIALDLLERMRDSFGLPDALFSPATLVCLQNYRWPGNVRQLKNEIQRM
                     LALADAPVLGPELLSPQVLHAGDESQQQELAFLGSVRGDLKERLDQLESQIIRETLTR
                     HRWNKTRAASELGLSRVGLRNKLVRYGLEPKA"
     gene            3177..>4677
                     /locus_tag="PKN55_11080"
     CDS             3177..>4677
                     /locus_tag="PKN55_11080"
                     /inference="COORDINATES: protein motif:HMM:NF024376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhnD/SsuA/transferrin family substrate-binding
                     protein"
                     /protein_id="HNX71830.1"
                     /translation="MAWWWVRMLLALCLASWLLPAGAQPVRLGVLAWLGEDRAAAQWA
                     PLLDALASRLPAHSLHARYLDLDAMDTAVARGELDFVITNPGHYVALEARHGVSRIAT
                     QQIAAGHDSAHAVAATVIVPATDSTRRRLADLRDARLAMVTPQAFGGYQVIWGELKRA
                     GVDPETGALTLRPTGYPMTRVVDALRAGQADAGVLRACLLEALQRTGQVRPGEFRVLS
                     PQHHAGEPCQRSSPIYPGWAFAAAHDTPPALSRAVLLALLALPDTDDVTRWGVPADYH
                     PVHDMLRELQIPPYDFLRQHRLENQVRRYWPWAAGVVALLLAWLAYTVRVEALVQRRT
                     RELSAALTARDQLAERVHAHQREMDHLSRLSVLGELSGSLAHELNQPLATIGNYARSL
                     KRRQANGQLDAAALIRAADEIASESERAAGVLAGIREFARKRARVREPHALATLTQRS
                     LSLFRGMLAHAPAITWQCSAEAAEAQVLADPLQIQQVLLNLLKNACDAQQ"
BASE COUNT          824 a         1513 c         1526 g          814 t
ORIGIN      
        1 tgcggtcgtg cgcctagcca ggatcgcttt gctgagtttt gttagccgct cttagtgttg
       61 cttacagcat gccgctcagc agcaggccac ccgtggcggc catgcctgcg cccagcacgc
      121 ggcgggcggt gctggtcaga tgctgattca gggtaaagcc ggcggcgtgc agtgcggcgg
      181 tggccacggc aaagccggcg ccatacagcc agaagctgcc atcggccggg gcttcaacac
      241 cgtgagcaaa gccatggaac acggcaaagc cggcggtcag cgccatggcc agcacggtgg
      301 gcatgcgcac cgacagcgac accagcaggc ccagcgccag caccgacaag gcaatgccgg
      361 tttcgatggc cggcaatggc atgcccagat ggccggcaac gccgcccagc agcatggcca
      421 gcacaaagct ggccggcaga gccaggcgcg ccgcgccacc cagcatggcg ccccagatgc
      481 ccacagccag catggccagc agatggtcgg cgccagacag cggatggctc aggccggcaa
      541 acagatcaaa atggctatgt gccccagtgt gggcatgggc cagcggtgcg gccagcacgg
      601 cggccagggc aagggcgttg cgaagcttca cggtgtcatc ctcttattgg atcaggcggt
      661 gtgatcgttg attttttcgg cgcggagccg aaagccgtgc atcaggcaaa cacaaaccgt
      721 gccagcccgg gcgcggcggc ggcggctggc tggcggcaga caagccacac gcaatcagcg
      781 gcttgtcggc cgtcggtgcg catcatggtg gcacaaggtg gataaatggc gtgcgttttg
      841 caaaaaaaca gaaaaccagg tttgcacttg ccggggttgg ccagccgttc cctcagccct
      901 ggctggaatc tgccgatttt ttcggcagca ccgacaacgc gccttgcacc agcccggcca
      961 ggtcggccag attgctgggg atcaccaccg tggtgccctg gcgggcaatc tggccaaatg
     1021 ccgccacata ttgctcggcc actttcagcg cggcggcacg gtcgccgcct tgttgttcca
     1081 gtgcgtgcgc cacgctggca atcgcttcgg cattggcctg caagcttttg cgacgttgcc
     1141 caattcggcg gccaaattca acaggccaat cttgtggcga atgacattct ggttgaaact
     1201 gctcatgggg ttgctccctg gtgcttttgc gctgttttga tgaagattcg cccctccatc
     1261 aaaacggcta ccctttggca aggccctact gtcagatcaa gtctgaactt ttacacttga
     1321 taaaattcgc cctagaaaat ctcccccatg tcttcgtgct gcatgcggct tgctttatat
     1381 gaaatgtcac aatccgtcct ggacaccatg gccgatgcca ccatggccgt gctggcctgg
     1441 ctgggcctga acaccgaata ctggccgccc gtcttgggtg tggcgctgtt tgtgtgggtg
     1501 ctgtcggcgc tctggtcgct gtacagaaat ctgggtggcg ttgatgcctc tggcacaagc
     1561 agcgattggg acatgagcga cacctcggac agcgcgagca gcagcgactg cggcgatagt
     1621 ggcggcggag ggtgtgattg cggtgggggc gacgactgaa acagccaccc cggccgaggg
     1681 ccgcgccgat ataatagccg ccttgttgtt gcatcccccg gagacgccat gtccaccacc
     1741 ctgcccgttg tcttgatcgt ggacgatgaa atccgctcgc tggaaaccct gcggcgcacg
     1801 ctggaagaag ccttcgaggt gttcaccgcc gccagcaccg acgaagcgct ggccattctg
     1861 caaaacgaat tcatccatgt ggtgatcagc gaccagcgca tgccggacat gaccggcgtg
     1921 gcgtttctca agctggtgcg cgagcgctgg cccgaggtgg cgcgcattat tttgtctggc
     1981 tacgccgaca acgccgacat catcgccgcc atcaacgaag ccggcatcta tcaatacctg
     2041 ctgaagccct ggcggccgga tgcgctgttg ctcaccgtgc agggcgcggc gcggctggcc
     2101 accctgcaac gcgaaaacga gctgctcaat gtcgagctga aagccggcga gccgtggatg
     2161 cgcagccggg tggcggccaa gcgccagcag gcgtcggacg cctttgccat gcacagcatc
     2221 gtgcgcgccg ccaacagccc gctgaaccgc atctgcgcct tgctgaccaa ggtggcggtg
     2281 cacgacatcg ccgtgctgat tgagggtgaa tctggcgtgg gcaaagagct gctggcgcgc
     2341 gcgctgcact acgccagcca ccgcgcccag cgcccatttg ttggcgaaaa ctgcgcggcg
     2401 ctgcccgagc aactgctgga aagcgtgctg tttggccaca agcgcggcgc atttaccggc
     2461 gcctatcagg atcatgtcgg gctgtttcag caggctgacg gcggcacgat ttttctggat
     2521 gaaatcggcg aaacctcgcc ggcttttcag gccaagctgc tgcgcgtgct gcaagaaggc
     2581 gaaatccgcc cggtgggcag ctcgcgctcg ctgcgggtgg atgtgcgcgt ggtgtcggcc
     2641 accaaccgca ccctggcgga tttggtgcag caggggcgtt ttcgggaaga tttatactat
     2701 cggctgtcgc cgtttgccgt gctcaccccg ccgctgcgcg agcggccaga agatatcccg
     2761 ctgattgcgc tggacttgct ggagcgcatg cgcgacagct ttggcctgcc cgacgcgctg
     2821 ttcagcccgg ccaccctggt gtgcctgcaa aactaccgct ggcccggcaa tgtgcgccag
     2881 ctgaaaaacg aaatccagcg catgctggcg ctggccgacg cgccggtgct gggcccggag
     2941 ctgttgtcgc cgcaggtgct gcacgctggc gacgaatcgc agcaacaaga attggctttt
     3001 ctgggcagtg tgcgcggcga cctgaaagaa cggctggacc aactggaaag ccagatcatc
     3061 cgcgaaaccc tcacccgcca ccgctggaac aaaacccgcg ccgccagcga gctgggcctg
     3121 tcgcgggtgg gcctgcgcaa caagctggtg cgctacggac tggagcccaa ggcctgatgg
     3181 catggtggtg ggtgcgcatg ctgctggccc tgtgcctggc cagctggctc ctgcccgcag
     3241 gggcgcagcc ggtgcggctg ggggtgctgg cctggctggg cgaggaccgc gccgccgcgc
     3301 aatgggcgcc gctgctggat gcgctggcca gccggttgcc ggcacattcc ctgcacgcac
     3361 gctatctgga tctggatgcc atggacaccg ccgtggcgcg cggcgagctg gattttgtca
     3421 tcaccaaccc ggggcattac gtggcgctgg aagcacgcca tggcgtcagc cgcattgcca
     3481 cccagcaaat tgccgccggc cacgactcgg cacacgcggt ggccgccacc gtgattgtgc
     3541 cggccacgga cagcacacga cgccggctgg ccgatctgcg cgatgcccgg ctggccatgg
     3601 tcaccccgca agcgtttggt ggctatcaag tgatctgggg cgagctcaag cgcgccgggg
     3661 tagacccgga aaccggcgcg ctgaccttgc gccccaccgg ctatccgatg acccgagtgg
     3721 tagatgccct gcgcgccggc caggccgacg ctggcgtgct gcgcgcctgt ctgctggaag
     3781 cgctgcagcg cacaggccag gtgcgtccgg gcgagtttcg ggtgctatcg ccacaacacc
     3841 atgccggaga accctgccag cgctcgtcgc ccatctatcc gggctgggca ttcgccgccg
     3901 cgcatgacac accgccggcc ttgtcccgtg ccgtgctgct ggcgctgctg gccttgccgg
     3961 acaccgatga tgtcacccgc tggggcgtgc ctgccgatta ccatccagtg cacgacatgc
     4021 tgcgcgaatt acagattccg ccttatgact tcctgcgtca gcaccggctg gaaaaccagg
     4081 tgcgccgcta ctggccatgg gctgccggtg tagtggcgct gctactggcc tggctggcct
     4141 ataccgtgcg ggtggaagcg ctggtccagc gacggacccg tgagctgtcg gccgcgctga
     4201 ccgcacgcga ccagctggcc gaacgggtac atgcccacca acgagaaatg gaccacctgt
     4261 cgcggctgtc ggtgctgggt gaattatctg gctcactggc gcatgagctg aaccagccgc
     4321 tggccaccat cggcaattac gcgcgcagcc tgaagcgccg tcaggccaat ggtcagctgg
     4381 acgcggccgc gctgatccgc gccgccgacg aaatcgccag cgaatccgaa cgcgccgctg
     4441 gcgtgctggc cggcatccgt gaatttgccc gcaagcgcgc acgggtgcgc gagccccatg
     4501 cgctggccac actgacccag cggtcactca gcctgtttcg cggcatgctg gcgcacgcgc
     4561 cggccatcac ctggcagtgc agcgccgagg cggccgaggc acaggtattg gccgacccct
     4621 tgcagattca gcaagtgctg ctcaacctgc tgaaaaacgc ctgtgacgcc cagcagg
//
LOCUS       DAOPYX010000233         4671 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_23490_length_4671_cov_8.252166, whole genome shotgun sequence.
ACCESSION   DAOPYX010000233 DAOPYX010000000
VERSION     DAOPYX010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 4671)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4671)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4671
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23490_length_4671_cov_8.252166"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..947
                     /locus_tag="PKN55_11085"
     CDS             <1..947
                     /locus_tag="PKN55_11085"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020701147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HNX71831.1"
                     /translation="YKWAWEKYLATCANHWMPQEVNMQRDIELWKSPNGLTDDERRLV
                     KRNLGFFVTADSLAANNIVLGTYRQITAPECRQFLLRQAFEEAIHTHAYQYIVESLGL
                     DESEVFNAYHEIQSIRNKDEFLIPFIDALTNPEFKTGTLETDQQLLKSLIVFACIMEG
                     LFFYVGFVQILALGRQNKMTGAAEQYQYILRDESMHCNFGIDLINTIKLENPQLWTDS
                     FAAEITELFRHAVDLEYAYAEDTMPRGVLGLNAPMFKEYLRFICNRRMTQIGLPELFP
                     GANNPFPWMSEMIDLKKEKNFFETRVTEYQSGGALSWD"
     gene            complement(1293..2459)
                     /locus_tag="PKN55_11090"
     CDS             complement(1293..2459)
                     /locus_tag="PKN55_11090"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HNX71832.1"
                     /translation="MLLLDPFTLFISNALLHLALMLALAIISRLAPDILAIRLWMRGH
                     GLMALAIVLMAVMALLSPEARPPLKLLIGGLLMSALLLMLAGLRHFFGLHPLPLALRV
                     GYVLALLGLAGLMQWSPDVPLAGVWQASVLIVLLLGCGHVLWRQRGHLRPIVLLACLA
                     SLGGMTLGLCGRLSLLVYSIFLGEQGLQWRQLSDVLGLMVAMIGGISMAMSFWLLISD
                     RMLHTLRRMAEEDPLTGVLNRRGFQRATARMLANQPGRVAVLMLDVDHFKRINDNYGH
                     AAGDQVLRCMGDALRDALLASDIFARLGGEEFCVVLGELDERNAEAIGERIRQRFQRK
                     TGLLSQAEGCTLSIGMAMGTPPVDSLAELMARADLALYHSKAEGRDQLSVYADG"
     gene            2784..4451
                     /gene="ettA"
                     /locus_tag="PKN55_11095"
     CDS             2784..4451
                     /gene="ettA"
                     /locus_tag="PKN55_11095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011136907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HNX71833.1"
                     /translation="MAQYVMSMLRVSKIVPPKRQIIKNISLSFFPGAKIGLLGLNGAG
                     KSTVLKIMAGVEKEYDGEVQWQPNLNIGYLPQEPQLDPEKTVREEVESGMGEVMGAQK
                     RLEEVYAAYAEPDADFDALAEEQARLEAIISAGAGDNVEHQLELAADALRLPPWDAKI
                     GPLSGGEKRRVALCKLLLSKPDMLLLDEPTNHLDAESVEWLEQFLCRFPGTVVAVTHD
                     RYFLDNAAEWILELDRGEGIPWKGNYSSWLEQKEARLEQEAKSEVARMKAMKQELEWV
                     RQNPKGRQAKSKARIARFEELSSFEHQKRNETQEIFIPVAERLGNEVIEFENVSKGFG
                     DRLLIDNLSFKAPPGAIVGIIGPNGAGKSTLFKMIAGKEQPDSGAVKIGQTVQMAFVE
                     QSREGLEGDKTVFEDVANGADILTVGKFEMPSRAYLGRFNFKGGDQQKKVGMLSGGER
                     GRLHLAKTLLKGGNVLLLDEPSNDLDVETLRALEDALLEFAGTVFVISHDRWFLDRIA
                     THILAAEGESQWTFFDGNYQEYEADKKKRLGEEGAKPKRIRYKPITR"
BASE COUNT         1068 a         1511 c         1319 g          773 t
ORIGIN      
        1 agtacaagtg ggcctgggaa aaatacctgg ccacctgcgc caaccactgg atgccgcagg
       61 aagtgaatat gcagcgcgac atcgaactgt ggaaaagccc caacggcctg accgacgacg
      121 aacgccgcct ggtcaagcgc aacctgggct tttttgtcac cgccgacagc ctggccgcca
      181 acaatatcgt gctgggcacc taccgccaga tcaccgcgcc ggaatgccgc cagtttctgc
      241 tgcgccaggc atttgaagaa gccatccaca cccacgccta tcaatacatc gtggaaagcc
      301 tgggcctgga cgaaagcgaa gtgttcaacg cctaccacga aatccagtcc atccgtaaca
      361 aggatgaatt tctgattccg ttcatcgacg cgctgaccaa cccggaattc aaaaccggca
      421 cgctggaaac cgaccaacag ctgctcaaat cgctgatcgt gtttgcctgc atcatggaag
      481 gcctgttctt ctacgtcggc tttgtgcaaa tcctcgccct gggccgccag aacaaaatga
      541 ccggcgccgc cgagcaatac cagtacatcc tgcgcgacga atccatgcac tgcaatttcg
      601 gcatcgacct gatcaacacc atcaagctgg aaaacccgca actgtggacc gacagcttcg
      661 ccgccgaaat caccgagctg ttccgccacg ccgtcgatct ggaatacgcc tacgccgaag
      721 acaccatgcc gcgcggcgtg ctgggcctga acgcgccgat gttcaaagaa tacctgcgct
      781 ttatctgcaa ccgccgcatg acccagatcg gcctgccgga actcttcccc ggcgccaaca
      841 accccttccc gtggatgagc gaaatgatcg acttgaagaa agaaaagaat ttctttgaga
      901 cgcgagtgac ggaataccag agcggtgggg cgttgagctg ggattgagtg ctgccgcttt
      961 gggcgacaga taattttgtt ctgagggagt cagccgctca agcctgcaca ggcgcaggct
     1021 tgagcgggct ttgtcgtgca aactcggccg ggctcaattg cccagcgccg agttcaagcg
     1081 tttctgctgg taaaacgtca gcaggcagtg ccgtccgcca catcccggcg ggcttctgcc
     1141 tggctggcgt agtctctatg ccataccagc tttaggctgc agcgcgccat cggggcgtta
     1201 tgccgggctg cgccctctcg cctacggctc gcaacacctg cgcactcacg gcaatcacgc
     1261 cagtaatgat tggtgtgagc cgctcctggc actcagccat cggcgtatac gctcagttgg
     1321 tcacggcctt cggccttgct gtggtaaagc gccagatccg cgcgcgccat cagctcagcc
     1381 aatgaatcaa ccggtggcgt gcccatggcc atgccgatgc tcagcgtgca gccttctgcc
     1441 tgactaagca ggccggtttt acgctgaaaa cgctggcgga tgcgctcgcc aatcgcctcg
     1501 gcattgcgct cgtcaagctc gcccagcacc acacaaaatt cctcgccgcc cagccgggca
     1561 aagatatcgc tggcgagcaa tgcatcgcgc agcgcatcgc ccatgcaacg caacacctga
     1621 tcgcccgccg catggccata gttgtcgttg atgcgcttga agtgatccac atccagcatc
     1681 agcaccgcca cccgacccgg ttgattggcc agcatgcgcg cagtggcgcg ctgaaaaccc
     1741 cggcggttga gcacgccggt aagcgggtct tcttcggcca tgcggcgcag ggtgtgcagc
     1801 atgcggtcag aaatcagcaa ccaaaagctc atggccatgc tgatgccgcc aatcatcgcc
     1861 accatcaaac ccagcacatc agacaattgc cgccactgca gaccttgctc acccaggaaa
     1921 atactgtaca ccagcaggct tagccggcca cacaggccca gggtcatgcc ccccagtgag
     1981 gccagacagg ccagcaggac aataggccgc aagtggccgc gctgccgcca caacacatgc
     2041 ccacagccca ggagcagaac aatcaagacg ctggcctgcc atacgccagc cagtggcaca
     2101 tccggcgacc attgcatcag gccagccagg ccaagcaggg ccagcacata gcccacacgc
     2161 aaggccagtg gcaaggggtg caggccaaaa aaatgacgca gcccggccag catcagcaac
     2221 agcgctgaca tcaataatcc gccaatcaac agcttgagcg gcggtctggc ctcgggcgac
     2281 agcagcgcca tcaccgccat cagcacaata gccagcgcca tcaggccatg gccgcgcatc
     2341 cacaagcgga tagccaggat gtcgggcgcc agccggctga tgatggccag cgccagcatc
     2401 agggccaggt gcagcaaggc attgctgata aataaggtaa aagggtctag taagagcacg
     2461 ggtttcctgt gccgacacgc ggcccggccg tcaaaaggcc aatcataaag aagagccgtg
     2521 ccgttataaa tcaaaaacgt gtccactgaa agcccgctcc gcatgttcac gggcctgtcc
     2581 cgccatctcc tggccccagg ctgcgctgcc gggaggacaa attcaggcca atccgcgcgc
     2641 catgcaccgc acccccgatg ccagccggcc ggggctttgc tacacttgcg gtttaccggc
     2701 cgcgccctcg cgcggtttcg cgccgaacgc gcccgcgcgt tcaccccggc gcctttacac
     2761 accaacggat tccagaccac accatggccc aatacgtaat gtccatgctg cgggtgagca
     2821 aaattgtgcc gcccaagcgc cagatcatca aaaacatctc gctgtcgttc ttccccggcg
     2881 ccaagattgg cctgttgggc ctgaacggcg ccggtaaatc caccgtgctg aaaatcatgg
     2941 ccggcgtcga aaaagaatac gacggcgaag tgcaatggca gcccaatctg aacatcggct
     3001 acctgccgca ggagccgcag ctcgacccgg aaaaaaccgt gcgcgaagaa gtggaaagcg
     3061 gcatgggcga ggtgatgggc gcgcaaaaac gcctggaaga agtctacgcc gcctacgccg
     3121 agccggacgc cgactttgac gccctggccg aagaacaagc ccgcctggag gccatcatct
     3181 cggccggcgc tggcgacaat gtggaacacc agctggagct ggccgccgac gcactgcgcc
     3241 tgccgccgtg ggacgccaaa atcggcccct tgtccggcgg tgaaaagcgc cgcgtggcgc
     3301 tgtgcaagct gctgctgtcc aaacccgaca tgctgctgct ggacgaaccg accaaccacc
     3361 tggacgccga atcggtggaa tggctggagc agttcctctg ccgcttccct ggcaccgtgg
     3421 tggccgtcac tcacgatcgt tacttcctgg acaacgccgc cgaatggatt ctggagctcg
     3481 accgtggcga aggcatcccc tggaagggca attactccag ctggctggag caaaaagaag
     3541 cccgtctgga gcaagaagcc aagtcggaag tggcccgcat gaaggcgatg aagcaagagc
     3601 tggaatgggt gcggcaaaac ccgaaaggcc gtcaggccaa atccaaggcg cggattgccc
     3661 gctttgaaga actgtccagc ttcgagcacc aaaagcgcaa cgaaacccag gaaatcttca
     3721 tcccggtggc cgagcgtctg ggcaacgaag tcatcgagtt cgagaacgtc agcaagggct
     3781 ttggcgaccg cctgctgatc gacaacctca gcttcaaggc cccgccgggc gccatcgtcg
     3841 gcatcatcgg ccccaacggc gccggtaaat ccaccctgtt caagatgatt gccggcaaag
     3901 agcagccgga cagcggcgcg gtgaaaatcg gccagaccgt gcaaatggcc tttgtcgaac
     3961 aaagccgcga aggcctggaa ggcgacaaaa ccgtctttga agacgtggcc aacggcgccg
     4021 acatcctcac cgtcggcaag ttcgaaatgc ccagccgcgc ctatctgggc cgctttaact
     4081 tcaagggcgg cgaccagcaa aagaaagtcg gcatgctctc cggcggcgaa cgcggccgcc
     4141 tgcacctggc caaaaccctg ctcaagggcg gcaatgtgct gctgctggat gaaccgtcca
     4201 acgacctgga cgtggaaacc ctgcgcgcgc tggaagacgc cctgctggaa tttgccggca
     4261 cggtgtttgt gatcagccat gaccgctggt tcctcgaccg catcgccacc cacatcctgg
     4321 cggcggaagg cgaatcgcaa tggaccttct ttgacggcaa ctatcaggaa tacgaagccg
     4381 acaagaaaaa acgcctgggc gaagaaggcg ccaagcccaa gcgcatccgc tacaagccga
     4441 tcacccgcta agcgtcgctc agaaaacgct ttctagtcgc cagtcaccat gatctgaacc
     4501 gactggtcgg cgcttcgacc gctgtacgct gtggacacac ctggattccc gccttcgcgg
     4561 gaatgacgcg gtgtcatgcg gcgagagctt cttcgtggcg catgaacgct tgtgtttcaa
     4621 cgtgactggc gactcgcgtg cttgtgtgac tttgctgcac ttgtttagga t
//
LOCUS       DAOPYX010000234         4652 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_23628_length_4652_cov_11.152056, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000234 DAOPYX010000000
VERSION     DAOPYX010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 4652)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4652)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4652
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23628_length_4652_cov_11.152056"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1130)
                     /locus_tag="PKN55_11100"
     CDS             complement(<1..1130)
                     /locus_tag="PKN55_11100"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM-DISM domain-containing protein"
                     /protein_id="HNX71834.1"
                     /translation="MPTLNWQMWEDPLGGRDTLVAWQSEHFRPLTAERMNPGFSSSVF
                     WLRTQLRNTRAEPVTYWLVLDNPRLEDVQLSQRAMGDMGPGITRVAGWRYPQFWREVS
                     ADMVVFPITVPGGGMEELMLRVESRSAVSLGTSLWQPLAFREQETNQHQLQGLMMGML
                     GAVAVYALIQGVVRRDTVFTLFALWLASFMCHALVFLGYGYRYFWTQGGAEVVRLTGV
                     MGASSALMFMLLSMSFLHFRQTLPPWMYRQAWLLAGVLVALMLWMSLGDYRMASSVLS
                     LTLLLGNVSWVLPCALACIRRTPNAKLFGVSIVVFWGAGVQWQLSLLGLLAPVSTMHD
                     GVWWFYNIALAITLLVGVTSRSMQLQRDHERTQRILLEIQSR"
     gene            complement(1364..2173)
                     /locus_tag="PKN55_11105"
     CDS             complement(1364..2173)
                     /locus_tag="PKN55_11105"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNX71835.1"
                     /translation="MNAAPFKNLSPRILLVDDAAEDRLLLADFLRQQGYRLYVAEDGL
                     DGYQKAVAIQPDLVLLDIHMPRCDGLATCRRLKADGQTSKIPVIFLTAAAMPQDRVAG
                     LNEGAVDYVTKPFDFEEVKLRVGIHLGLANKVHAPARSSSAAPEDEAVPGHASNLDKV
                     LFASARKILRESLASAPNLSELATAVGTNTRRLNEAFKHCAGATVFDYLRELRLSEAR
                     RLLAESALPVQQIASHCGYSNQANFSTAFRERFGLTPSDFRRESEPHDARA"
     gene            complement(2503..3270)
                     /gene="tcdA"
                     /locus_tag="PKN55_11110"
     CDS             complement(2503..3270)
                     /gene="tcdA"
                     /locus_tag="PKN55_11110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014085636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HNX71836.1"
                     /translation="MSAPDDWARRFGGIGRLYGEAALARFRHAHVCVIGVGGVGSWAA
                     EALARSAIGELTLIDLDHVAPSNINRQAHALSDTLGMAKVDAMAERLLQINPQLVIHR
                     VDEFIEPDTVGQLLSTQLDYVVDAIDSLRAKAALIAWCRRHKVPVVVSGGAGGKTDPT
                     RIVVDDLSRTTDDPMASKLRYTLRRQHGFAREGKFGVPCVFSTEPLIRPSAACEAGAA
                     GAQGLNCAGFGSVMGVTAGFGLAAASRVLRDLARQAR"
     gene            complement(3282..3920)
                     /gene="pyrE"
                     /locus_tag="PKN55_11115"
     CDS             complement(3282..3920)
                     /gene="pyrE"
                     /locus_tag="PKN55_11115"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003759231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HNX71837.1"
                     /translation="MSDFRQDFIRFAVEEQVLRFGEFVTKAGRLSPYFFNAGLFNHGA
                     SLLKLARFYAQAIRASGVEFDMLFGPAYKGITLAGATAMMLAEDGLDIPFAFNRKEAK
                     DHGEGGVLIGAPLQGRVLIIDDVISAGTSVRESVELIRAHGATPAAVSIALDRKERGK
                     GELSAVQEVQRDYGMPVVAIATLDDLLGYLAHSPDLADNQAKVQAYRDQYGV"
     gene            3997..>4652
                     /locus_tag="PKN55_11120"
     CDS             3997..>4652
                     /locus_tag="PKN55_11120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HNX71838.1"
                     /translation="MPRIISANLNGIRSATQKGFFDWLAGQHADVVCVQELKAQHDDL
                     TERMRAPDGLQGWFHPAEKKGYSGVGLYARREPDRLDIGLGIADIDAEGRYLRADFGA
                     LTVVSLYLPSGSSSDDRQQVKFDFLARFLPYLRELAASGRDVVVCGDWNIAHQEIDLK
                     NWKGNLKNSGFLPEERAWMTQLLGEEGWRDIWRQLYPEIPGYTWWSNRGQAYAKDVGW
                     R"
BASE COUNT          931 a         1480 c         1432 g          809 t
ORIGIN      
        1 tggcggctct ggatttccag caggatgcgc tgggtgcgct cgtggtcgcg ctgcaattgc
       61 atggagcggc tggtgactcc caccagcagg gtgatggcca gggcgatatt gtaaaaccac
      121 cataccccgt catgcatggt ggataccggc gccagcagcc ccagcaggga caattgccat
      181 tgcacgccag ccccccagaa caccacgatg ctgacgccaa acagcttggc gttgggcgtg
      241 cggcggatac aggccagtgc acatggcaat acccaactga cattgcccaa gagcagggtc
      301 agcgacagca cgctggaggc catgcggtaa tcacccaggc tcatccacag catcaacgcc
      361 accaacaccc cggccagcaa ccaggcttgg cgatacatcc agggcggcag ggtctggcga
      421 aagtgcaaaa aggacatcga cagcagcatg aacatcagcg ccgagctggc tcccatcacc
      481 cctgtcagcc gcaccacctc ggcgccaccc tgtgtccaga aataccggta gccatagccc
      541 agaaacacca gcgcatggca catgaagctg gccagccaca gggcaaacag ggtgaacacc
      601 gtatcccggc gcaccacccc ctgaatcagt gcatatactg ccaccgcgcc cagcatgccc
      661 atcatcaggc cttgcaactg gtgctggttg gtttcctgct cgcgaaaggc cagcggttgc
      721 cacaggctag tgcccagaga gacagcgctg cggctttcta cacgcagcat cagctcttcc
      781 atgcctccac caggcacggt gatcggaaac accaccatgt cggcactgac ctcacgccag
      841 aactgcgggt agcgccaccc tgccacccgg gtgatgcccg gccccatatc gcccatggcg
      901 cgctggctga gctgcacgtc ttccagccgg ggattgtcca gaaccagcca gtaagtcacc
      961 ggctcggcgc gggtgttgcg taattgcgtt cgtagccaga ataccgagga cgaaaatccc
     1021 ggattcatgc gctcagcggt cagtgggcga aagtgctcgc tttgccaggc caccagcgtg
     1081 tcgcgcccgc ccagcgggtc ttcccacatc tgccagttca acgtgggcat gctgctggcg
     1141 ccggcaggcc aggccagcca ggcaagcgca atcaagcaga cacgcagcac agcgttcatc
     1201 gggacagtca gggaaaatgg acacgtgtgc attatgcctg catcccgccg ggctggcgcg
     1261 tccacccgcg ggggcggctg ccaatctggc catggcggga tggcgcaggc cagccgggtg
     1321 atcagacggt gtactgacgt gggctggtca acgaggaggg atgtcaggct cgcgcgtcgt
     1381 gcggctcgga ttcgcggcgg aaatcgctgg gcgtcagacc aaagcgctca cgaaaggcgg
     1441 tagaaaaatt ggcctgattg ctataaccgc agtggctggc gatctgttgc acgggcagtg
     1501 cgctttccgc cagcagccgg cgagcttctg acaggcgcaa ctcgcgcagg tagtcgaaca
     1561 ccgtggcgcc agcgcaatgc ttgaaggcct cgttcagccg acgggtattg gtgcccacgg
     1621 cggtggccag ttcactcaga ttgggggcag aagccagact ctcgcgcagg atcttgcgtg
     1681 ccgaagcaaa cagaaccttg tcgagattgg aagcgtgacc aggcaccgct tcatcttccg
     1741 gggctgcgga agacgagcgg gccggcgcgt ggaccttgtt ggccagaccg aggtggattc
     1801 ctacccgcaa cttgacctct tcaaaatcaa aaggtttggt tacgtagtcc accgcgcctt
     1861 cgttcagacc ggcaacccgg tcttgcggca tggcagcggc ggtgagaaaa atcaccggga
     1921 tcttgctggt ttgtccgtct gccttgaggc ggcgacaggt ggctaatccg tcgcagcgtg
     1981 gcatatgaat atccagcaag accaagtctg gctgaatggc aaccgctttc tggtaaccat
     2041 ccaggccgtc ctccgccaca taaaggcgat agccttgttg tcggaggaag tctgcgagca
     2101 ggagccgatc ttcagcggcg tcatccacca gcaggatacg aggagagagg tttttaaacg
     2161 gggcagcgtt catggatata ttgtggcata gcccccccct gtgtcacggg gggcgccggg
     2221 ctttttacct ggctttaccg cagtttttcc cttctaacaa aaactttctg cacttagcca
     2281 aaagaagcca tcccggccgg aaaaaccgtt agcctgatgc ggtttttatc tggctgacag
     2341 cgtgctgaca agctgatttt gtctgactga cgctgcaatc ttgttctgcg taaatagcat
     2401 tttttgatgt ctttggcgtg gcttctggtc gcgtggtgtt gtcatctggc gtctgggcgc
     2461 atttcccctg gcagctcggc ttgatgtacg ggccggccgc ggctaacgcg cctggcgtgc
     2521 cagatcgcgc agcacccggc tggccgccgc cagaccaaaa ccagcggtca cccccatcac
     2581 cgaaccaaac ccggcgcagt tcaagccctg agcgccagcc gcgcccgcct cgcaggcggc
     2641 gcttgggcgg atcaaaggct cggtggaaaa cacgcagggc acgccaaact tgccttcgcg
     2701 ggcaaagcca tgctggcggc gcagggtgta gcgcagcttg gaggccatcg ggtcgtcggt
     2761 ggtgcgcgac aaatcatcca ccacaatgcg ggtggggtcg gtcttgccgc cggcgccgcc
     2821 gctgaccacc actggcacct tatggcggcg acaccaggca atcagcgccg ccttggcgcg
     2881 caggctgtcg atggcgtcca ccacataatc cagctgggtg gacaacagct ggcccaccgt
     2941 gtccggctcg atgaactcat ccacccggtg aatcaccagc tgcggattga tctgcaacag
     3001 gcgctcggcc atggcgtcca ccttggccat gcccagcgtg tcgctcagcg cgtgcgcctg
     3061 gcggttgata ttggacgggg cgacatggtc cagatcaatc agcgtcagct cgccaatcgc
     3121 gctgcgcgcc agcgcctcgg ccgcccacga gccgacgccg cccacgccaa tcacgcagac
     3181 atgcgcatgg cgaaagcgcg ccagcgccgc ctcgccatac agccggccaa tgccgccaaa
     3241 gcggcgcgcc caatcgtcgg gcgcagacag cggctcgctc atcacacgcc gtactggtcg
     3301 cggtaagcct gcaccttggc ctggttgtcg gccaaatccg ggctgtgggc cagataaccg
     3361 agcaaatcgt ccagcgtggc gatggccacc accggcatgc cgtagtcgcg ctgcacttcc
     3421 tgcacggcgg acaattcgcc cttgccgcgc tccttgcggt ccagcgcaat cgacaccgcc
     3481 gccggggtgg cgccatgggc gcggatcagc tcgaccgatt cgcgcaccga ggtgccggcg
     3541 gaaatcacgt cgtcgataat cagcacccgg ccttgcagcg gcgcgccaat cagcacgccg
     3601 ccctcgccgt ggtctttggc ttctttgcgg ttgaaggcaa acggaatgtc caggccgtct
     3661 tcggccagca tcatcgccgt ggcgccggcc agggtgatgc ccttgtaggc cgggccaaac
     3721 agcatgtcga attccacgcc gctggcgcgg atggcctggg cgtaaaaacg cgccagcttg
     3781 agcaagctgg cgccgtggtt gaacaggccg gcgttaaaga aatacggcga caggcggccg
     3841 gccttggtga caaactcgcc aaagcgcaac acctgctctt ccacggcaaa acgaataaaa
     3901 tcctggcgaa aatcgctcat tgggcatcct caatttcaat acttggccat gctggcgccg
     3961 gcaaaaacgg gcgacagcat agcatggaga cttctcttgc cacgaatcat cagcgccaac
     4021 ctcaacggca tccgctcggc cacgcaaaaa ggctttttcg actggctggc cggccagcac
     4081 gccgacgtgg tgtgcgtgca ggaactcaag gcccagcacg acgacctgac cgagcgcatg
     4141 cgcgcgcccg acggcctgca aggctggttt cacccggccg agaaaaaagg ctatagcggc
     4201 gtgggcctgt acgcgcggcg cgagccggat cggctggaca tcggcctggg cattgccgac
     4261 atcgacgccg aagggcgcta tctgcgcgcc gactttggcg cgctgaccgt ggtgtcatta
     4321 tacctgccgt ccggctccag ctcggacgac cgtcagcagg tgaagtttga ttttctggcg
     4381 cgctttctgc cttatttgcg cgagctggcc gccagcggcc gcgatgtggt ggtgtgcggc
     4441 gactggaata tcgcccatca ggaaatcgac ctgaagaact ggaagggcaa tctgaaaaac
     4501 agcgggtttt tgccggaaga gcgcgcctgg atgacccagc tattgggcga agaaggctgg
     4561 cgcgacatct ggcgccagct ttacccggaa attcccggct acacctggtg gagcaatcgc
     4621 ggccaggcct acgccaagga cgtgggctgg cg
//
LOCUS       DAOPYX010000235         4644 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_23691_length_4644_cov_8.838745, whole genome shotgun sequence.
ACCESSION   DAOPYX010000235 DAOPYX010000000
VERSION     DAOPYX010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 4644)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4644)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4644
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23691_length_4644_cov_8.838745"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            548..1129
                     /gene="ssuE"
                     /locus_tag="PKN55_11125"
     CDS             548..1129
                     /gene="ssuE"
                     /locus_tag="PKN55_11125"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: protein motif:HMM:TIGR03567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase activity [Evidence
                     IEA];
                     GO_process: GO:0044273 - sulfur compound catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HNX71839.1"
                     /translation="MNVLAISGSPSATSKTLRLLDPLEQALNAADIELRRVSVSDFPA
                     EAALLARFDHPAVQGFHRQLAQASGVVVLTPVYQSAYAGALKVLLDLIPEGGLAGKPV
                     LPLVSGGSAGHLLAVDYALKPVLSALKASEIHQGVFAVSGDIGVDADGGLALAPALRA
                     RLDEAAQLFASQLSARRRAQQAPASWSGLRVCL"
     gene            1185..2120
                     /locus_tag="PKN55_11130"
     CDS             1185..2120
                     /locus_tag="PKN55_11130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018747174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HNX71840.1"
                     /translation="MKPVFRLASALLLAASLSSAWALELNIGYQKSAINLASLKAQGV
                     LEQRLKPLGVEVKWFEFQAGPPILEAMNAGSVSLGMTGDSPPIFAQSAGAEIVYIGQE
                     PAKPESSAILVPAASSIRTLADLKGKKIAFTKGSSAHYLTVSALQKAGLSYADIQPVY
                     LSPSEARAALERGSVDAWTIWDPYYAAAQRAGGVRVLATGQGLTSNNTFYLAARKFAR
                     ANPKVIDVVFKALTENDRQLAGEVKKVAATLSGYTGLDAGVYETMLERHPSYRVDYLT
                     PAVLREQQKIADTFHGLGLIPRPIQVHEAAWRPGR"
     gene            2178..3311
                     /gene="ssuD"
                     /locus_tag="PKN55_11135"
     CDS             2178..3311
                     /gene="ssuD"
                     /locus_tag="PKN55_11135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019922479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008726 - alkanesulfonate monooxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
                     /protein_id="HNX71841.1"
                     /translation="MNVFWFFPTHGDGRFLGSPEGARTVDHDYLSQIARAAESLGFDG
                     ALIPTGRSCEDPWVTASSLIPLTRKLKFLVAVRPGIMSPTVAARMAASFDRHSNGRLL
                     INVVAGGDPVELAGDGCWLSHDERYEAASEFLDVWKGVLSGQVVDLEGKHITVKGAQQ
                     LFPPVSQPHPPVYFGGSSPAAHDLAARHVDLYLSWGEPLDEVAKKIADLRQRAAAYGR
                     TLRFGLRVHVIVRDSTEEAWRAADALISKLDDQVIANAQHTLGRADSEGQRRMAALHG
                     GRRDRLEIAPNLWAGVGLVRGGAGTALVGDGPTVAARLREYAELGIDTFVLSGYPHLD
                     EAYRVAELVFPHLPGRAQPARTGGHAAGEVMANQHVPRLAAAS"
     gene            3325..4098
                     /gene="ssuC"
                     /locus_tag="PKN55_11140"
     CDS             3325..4098
                     /gene="ssuC"
                     /locus_tag="PKN55_11140"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019922480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter permease
                     SsuC"
                     /protein_id="HNX71842.1"
                     /translation="MNTSWFSRLIPWLAPFTLLALWQLGSSLGWINHRVLPAPLAVLT
                     SGWGLLASGELFRHLASSFGRAASGFVIGGGLGLIFGLIAGLSRLGEALFDTTLQMLR
                     NIPPLAILPLVILWFGIDESAKVFLVAFGTLFPVYLNTYHGIRSIDPALLEMARSYGV
                     SGRELFRHVVLPGALPSILVGVRYALGIAWILLIVAETLSATSGIGYLASNAREFLQT
                     DIVVLSILLYALLGKAVDSLARWLERRWLQWHPSYFQPA"
     gene            4125..>4644
                     /locus_tag="PKN55_11145"
     CDS             4125..>4644
                     /locus_tag="PKN55_11145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018747177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNX71843.1"
                     /translation="MKLDDLPSAEHAPIVLSGLTRRFGERVVLDGLNLSIPAGQFLAV
                     VGRSGCGKSTLLRLLAGLDRADGGELRQPAQQSVRVMFQEARLLPWKSVLDNVALGLA
                     PAQREQARDALAEVGLADRADEWPARLSGGQRQRVALARALVHQPRLLLLDEPLGALD
                     ALTRLDMQGLIER"
BASE COUNT          708 a         1484 c         1575 g          877 t
ORIGIN      
        1 gaggttaaat ccgcctgaaa acgcccggct tgccccgggt catgcagggc aatatcaaac
       61 agcgtatcgg tggcctgacg ccagatggcg taattctgct cagccggcaa atcctggctg
      121 gtgaaatgaa tcaggggcag gcggtcttca cctgtgctgg acatcggtgc gctcgtgtga
      181 gaatgatccg aaagatattc tgccggatca taccaccatt cattatccgc actgtgacag
      241 gccagacagc acatgatttt gcctggcctg tacgcaaacg ccagattttg taccactcca
      301 cgttttgtcc ctttgtgcgg ccggcgagag cgcacctgac cagatatgcc gctcacaaaa
      361 ccctctcggc gttgttgccc agtacttgta ctggcctgcg gctgcgcgcc tggccaggcg
      421 tgctccgctg agtttcgtga gcaactctta gcagaaattc tgctaacgat gtgcaattcc
      481 cttgtttaaa gcgcataaaa actgcgtgct aaggtgcggc catcttgatt gcattctggc
      541 ttatccgatg aacgttctcg ccatctctgg cagccccagc gccacctcga aaaccctgcg
      601 tctgctcgac ccgctggagc aggcgctcaa cgccgccgac atcgagctgc gccgtgtgtc
      661 ggtcagtgat tttccggccg aggcggcgct gttggcgcgc tttgaccatc cggccgtaca
      721 gggttttcac cgccagctgg cgcaggccag cggcgtggtg gtgctcaccc cggtgtacca
      781 gtcggcctac gccggcgcgc tgaaagtgct gctggatttg atcccggaag gcgggttggc
      841 cggcaagccg gtgttgccgc tggtcagcgg cggctcggcc gggcacctgc tggcggtgga
      901 ttacgcgctc aagccggtgc tgtcggcgct gaaagccagc gagattcatc agggcgtgtt
      961 cgcggtcagc ggcgacatcg gcgtggacgc cgacggcggc ctggcattgg cgccggcgct
     1021 gcgcgcccgg ctggacgaag ccgcccagct gtttgccagc cagctgtcgg cgcgacggcg
     1081 cgcccagcag gcgccggcct cgtggtctgg cctgcgggtg tgtctgtgag cgcgcgccgc
     1141 tgtgtctggg cgccgttgcg cgacgttgat cagggagaca tgtcatgaaa ccggtttttc
     1201 gtctggccag cgcgctgctg ctggcggcat cgctgtcttc agcctgggcg ctggagctga
     1261 acatcggcta ccagaaaagc gccatcaatc tggcttcgct gaaagcgcag ggcgtgctgg
     1321 agcaaaggct caagccgctg ggggtggagg tgaagtggtt tgaatttcag gccggcccgc
     1381 cgattctgga ggcgatgaac gccggcagcg tcagcctggg catgaccggc gattcgccgc
     1441 cgatctttgc ccagtctgcc ggcgccgaga ttgtctacat cggccaggag ccggccaagc
     1501 cggaatcctc cgccatcctg gtgccggccg cctcgtcgat tcgcacgctg gcggatctga
     1561 agggcaagaa aatcgccttc accaagggct ccagcgccca ttacctgacg gtgagcgcgc
     1621 tgcaaaaagc cggcctgagc tacgccgaca ttcagccggt gtatctgagc ccgtccgagg
     1681 cgcgcgccgc gctggagcgc ggcagcgtgg acgcctggac catctgggat ccgtactacg
     1741 ccgccgccca gcgcgcgggc ggcgtgcgcg tgctggccac cggccagggg ctgaccagca
     1801 acaacacgtt ttatctggct gcgcgcaagt ttgcccgcgc caaccccaag gtgattgacg
     1861 tggtgttcaa ggcgctcacc gaaaacgacc gccaactggc tggcgaggtg aaaaaagtgg
     1921 ccgccacctt gtccggctac accggcctgg acgctggcgt gtacgaaacc atgctggagc
     1981 gccatcccag ctatcgggtg gattacctga cgcctgcggt gctgcgcgag cagcaaaaga
     2041 tcgccgacac ctttcatggt ctggggctga tcccgcgtcc gattcaggtg cacgaagccg
     2101 cctggcggcc aggccgctga gcgccggctc ggggtttccg cccatcaccg aattcaatct
     2161 ggcaggagtg tattgcaatg aatgtgttct ggtttttccc cacccatggc gacggccgct
     2221 ttctgggcag cccggaaggc gcgcgcaccg tggaccatga ttatctgagc cagattgccc
     2281 gcgccgccga gtcgctgggt tttgatggcg cgctgattcc caccggacgc tcgtgcgaag
     2341 acccgtgggt gaccgcgtcc agcctgattc cgctgacccg caagctgaag tttctggtgg
     2401 cggtgcgccc cggcatcatg tcgcccaccg tggcggcgcg catggcggcc agttttgacc
     2461 gtcattccaa tggccgcttg ctgatcaatg tggtggccgg cggcgacccg gtggagctgg
     2521 ccggcgacgg ctgctggctg agccacgacg agcgctacga ggcggccagc gagtttctgg
     2581 atgtctggaa gggcgtgctg tccggccagg tggtggacct ggaaggcaag cacatcaccg
     2641 tcaaaggcgc gcaacagctg tttcccccgg tcagccagcc gcatccgccg gtgtattttg
     2701 gcggctcgtc gccggcagcg cacgacctgg ccgcccgcca tgtggatttg tacctgagct
     2761 ggggcgagcc gctggacgag gtggcgaaaa aaatcgccga cctgcgccag cgcgccgccg
     2821 cctatggccg cacgttgcgc tttggcctgc gcgtgcacgt gattgtgcgc gacagcaccg
     2881 aagaagcctg gcgcgcagcg gatgccttga tcagcaagct ggatgaccag gtgatcgcca
     2941 acgcccaaca caccctgggc cgcgccgatt ccgaaggtca gcgccggatg gcggccttgc
     3001 acggcggccg ccgcgaccgg ctggaaatcg cccccaatct gtgggcgggc gtgggcctgg
     3061 tgcgcggcgg cgccggcacg gcgctggtgg gcgatggccc caccgtggcg gcgcggctgc
     3121 gcgaatatgc cgagctgggc atcgacacct ttgtgctgtc cggctatccg cacctggacg
     3181 aagcctatcg ggtggccgag ctggtgtttc cgcacctgcc gggccgcgcc cagccggcgc
     3241 gcactggcgg ccacgcggcc ggcgaggtga tggccaacca gcatgtgccg cgcctggcgg
     3301 cggcgtcgtg aacggagcat ccgcatgaac acttcctggt tttcccggct gattccctgg
     3361 ctggcgccgt tcaccctgct ggcgctgtgg cagctcggct ccagcctggg ctggatcaat
     3421 catcgcgtgc tcccggcgcc gctggcagtg ctgacttccg gctggggctt gctggccagc
     3481 ggcgagttgt ttcgccatct ggccagcagc tttggccgcg ccgccagcgg ctttgtcatc
     3541 ggcggcggcc tgggtttgat ttttggcctg atcgccggcc tgtcgcggct gggcgaggcg
     3601 ctgttcgaca ccaccctgca aatgctgcgc aacatcccgc cgctggcgat tttgccgctg
     3661 gtgatcttgt ggtttggcat tgatgaatcg gccaaggtgt ttctggtggc gttcggcacg
     3721 ctgtttccgg tgtacctgaa cacctatcac ggcatccgct cgatcgaccc ggccttgctg
     3781 gaaatggcgc gcagttatgg cgtgtccggc cgcgagctgt tccgccatgt ggtgctgcct
     3841 ggcgcgctgc cgtcgattct ggtgggcgtg cgctacgcgc tgggcatcgc ctggatcttg
     3901 ctgatcgtgg cggaaaccct gtctgccacc agcggcattg gctatctggc ctcgaatgcc
     3961 cgcgagtttt tgcaaaccga cattgttgtg ctgtccatcc tgttgtacgc cctgctgggc
     4021 aaggcggtgg attcgctggc ccgctggctg gagcgccgct ggctgcagtg gcatccctcg
     4081 tacttccagc ccgcgtgacg ccgtgcagaa gaaaggaaac cgtgatgaaa cttgacgacc
     4141 tgcccagcgc agaacacgcg ccgattgtgt tgtccggcct gacgcgccgc tttggcgaac
     4201 gggtggtgct ggatgggctg aacctgagca ttccggccgg ccagttcctg gctgtggtcg
     4261 gccgctccgg ctgcggcaag agcacgctgc tgcgcctgct ggccgggctg gatcgcgccg
     4321 acggcggcga gctgcgccag ccggcgcagc aaagcgtgcg ggtgatgttt caggaagcgc
     4381 ggctgttgcc gtggaagtcg gtgctggaca acgtcgccct gggcctggcc ccggcgcagc
     4441 gcgagcaggc gcgagacgcg ctggccgaag tcggcctggc cgaccgcgcc gatgaatggc
     4501 cggcgcgctt gtccggcggc cagcgccaac gggtggccct ggcccgcgcg ctggtgcacc
     4561 agccgcgcct gttgctgctg gacgaacccc tgggcgcgct ggatgcgctc acccggctgg
     4621 acatgcaggg gctgatcgaa cggc
//
LOCUS       DAOPYX010000236         4632 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_23787_length_4632_cov_12.762290, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000236 DAOPYX010000000
VERSION     DAOPYX010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 4632)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4632)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4632
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23787_length_4632_cov_12.762290"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(109..957)
                     /locus_tag="PKN55_11150"
     CDS             complement(109..957)
                     /locus_tag="PKN55_11150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011134915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HNX71844.1"
                     /translation="MRQHPLFYALAAIVLWASLATLGASVAALPPFFTVGVSLMVGSL
                     CAIRHWRQWRVPPATLLLGVVGLFGYHFFLFVAFRLAPAVEANLLNYLWPLLIVLLSP
                     VLLPGYHLNTRHILGALAGFVGAALVVTGGKLSIDLTALPGYGCAALAALLWAGYSLL
                     TKRVPAFPTAAIGGFCLLSGALSLACHALMEPAVHPSASQWLTLLLLGAGPMGLAFFL
                     WDKALKLGDPRQIGALSYLTPLLSTLLLVASGRGQLGPTTGAAIALIVGGALAGTVDW
                     KGLRRR"
     gene            complement(1011..1922)
                     /locus_tag="PKN55_11155"
     CDS             complement(1011..1922)
                     /locus_tag="PKN55_11155"
                     /inference="COORDINATES: protein motif:HMM:NF013246.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin family ion channel"
                     /protein_id="HNX71845.1"
                     /translation="MIIRPRPHWLAILFARRGSVLPSILPQLGLVTALATIITTSHGT
                     VLGWKTPLTPMPFTLVGIALAIFLGFRNSASYDRYWEARKFWGQGLNECRTLTRQALS
                     LMDGQVDVRPFVYGQIAFIHALRGYLRRVPVERELAALLPAELLARMREAHYPPTLIL
                     VWLGQWLHEQRRAGHLQAVLAAKMEDALSGLNQVQGGCERIVSSPIPFAYTVILYRTV
                     GVYCLLLPFGLVDTLGWMTPLVTAFVSYTFFAQETLLSEIEEPFGNAENDLPLDALSI
                     TIERTLREMLGEATLPDMPAAKDFVLT"
     gene            complement(1978..2688)
                     /locus_tag="PKN55_11160"
     CDS             complement(1978..2688)
                     /locus_tag="PKN55_11160"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011133944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0140098 - catalytic activity, acting on
                     RNA [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase"
                     /protein_id="HNX71846.1"
                     /translation="MELERLLHSQGFGSRKACRALIEAGRVALAAGPCLNPKTPVSAD
                     LAFWVDEQPWQAASKVYLLLNKPTGYECSRAPQHHLPVLRLLPAPYVERGVQPVGRLD
                     ADTTGLLLLSDDGQFIHHFTSPRRHVPKTYAVSVRHPLDDTQLTALRQGVQLHDEPAP
                     LAALACAARDSHTLLLTIGEGKYHQVKRMVAAAGNRVDALHREAMGELVLPADLAPGQ
                     WRELTAEDFSQLGWPCGG"
     gene            complement(2750..3328)
                     /locus_tag="PKN55_11165"
     CDS             complement(2750..3328)
                     /locus_tag="PKN55_11165"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018152186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNX71847.1"
                     /translation="MTSPIFERYHVLVVPGWQNSGPEHWQSRWQDAHPAWQRVQQADW
                     LTPSRADWVATLDAAVRAADRPVLLVAHSLGCATTAHWAAQHPDSAARVVAAWLVAPA
                     DTERADAPPAVAGFAPLPRAPLPFASQIIASDNDPYCAAARADALARQWGSALTVLPA
                     AGHINTDAGFGPWPEGLAALADWLATLPLAQP"
     assembly_gap    3434..3533
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3534..4539)
                     /locus_tag="PKN55_11170"
     CDS             complement(<3534..4539)
                     /locus_tag="PKN55_11170"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNX71848.1"
                     /translation="MSVPSVSSDASLPDGAQPVTGRPARMPLLALLDPSLMAQYAPRP
                     PAPPKPVALPPAAPGRPGRRKAVSHDPQLAHFNARFVAAGHPADSLRLTPQQQVAASF
                     DEWRLSSQFAPILAPGAATPFAYKAQLRISGPAGHSVAPPLLFAVERAPSRTVLLDRL
                     CRVTHCLNFVRHGQPDAALHLNVHALHLINLPDGHGEFFAEAIAELGLRPSQLTLEIV
                     EDAEVDLLRLQRCMDSYRRFGFKLALDDFGASELSLPRVWLLEPDAVKLHERLLHRAL
                     SHPAARDALPRAVDILHAAGCQVIAEGVDTARHAELAGQAGCDGLQGRWVGPLHSRPQ
                     A"
BASE COUNT          840 a         1469 c         1581 g          642 t
ORIGIN      
        1 cccaaaacaa accgccacgg cccctatcac cacgcaaaca cccagcctga gcggataccc
       61 ccatccgctc agccgaaaac acccccccgc gccgcttccc aagcgggttt accgccgccg
      121 caaccccttc caatccactg tccccgccag cgcgccgccg acaatcagcg caatcgccgc
      181 gccagtggtc gggccgagtt ggccacggcc gctggctacc agcagcaggg tggacaacag
      241 cggggtgagg tagctcagcg cgccgatctg gcgggggtcg cccagtttca gcgctttgtc
      301 ccacagaaag aaggccaggc ccatcgggcc ggcgccgagc agcagcaggg tcagccattg
      361 gctggcgctg gggtgcacgg cgggttccat cagtgcgtgg caggccagcg acagcgcgcc
      421 ggatagcagg caaaagccgc cgatggcggc ggtgggaaaa gccggcacgc gtttggtcag
      481 cagcgagtag ccggcccaca acagcgcggc cagggcggcg cagccgtagc cgggcagggc
      541 ggtgaggtcg atgctgagct tgccgccagt gaccaccagc gcggcgccga caaagccggc
      601 cagcgcgccc aggatgtggc gggtgttcag gtgataaccc ggcagcagca ccggcgagag
      661 cagcacgatc agcagcggcc acaggtagtt cagcagattg gcttccaccg ccggggccag
      721 acgaaaggcg acaaacaaaa agaaatggta gccaaacagc cccaccacgc ccagcagcag
      781 ggtggcgggc ggcacgcgcc attgtcgcca gtggcggatg gcgcacaggc tgcccaccat
      841 caggctgacg ccgacggtga aaaacggcgg cagcgcggcc accgaggcgc cgagggtggc
      901 cagcgaggcc cacagcacga tggcggccag ggcgtagaac agcggatgtt gacgcatggg
      961 acacaaaccg ttggataaaa catccgaccg taccagattg gccggcaaga tcaagtcagc
     1021 acaaagtcct ttgccgctgg catgtccggc aatgtcgctt cgcctagcat ttcgcgcagg
     1081 gtgcgctcga tggtgatgga cagcgcgtcc agcggcaggt cgttctcggc gttgccaaag
     1141 ggttcttcga tttcgctgag cagggtttcc tgggcaaaaa aggtatacga gacaaacgcc
     1201 gtcaccagcg gggtcatcca gcccagggtg tccaccaggc caaacggcag cagcaggcag
     1261 tacacgccga cggtgcgata caaaatcacc gtgtaggcaa acggaatcgg gctgctgaca
     1321 atgcgctcgc agccgccttg cacctgattg aggccagaca gcgcgtcttc catcttggcg
     1381 gccagcacag cttgcagatg gccggcgcga cgttgttcat gcagccattg ccccagccac
     1441 accagaatca gcgtgggcgg atagtgggcc tcgcgcatcc gcgccagcag ctcggccggc
     1501 agcagcgccg ccagttcgcg ttccaccggc acccggcgca gatagccgcg cagggcgtgg
     1561 ataaaggcaa tctgcccata cacaaacggc cggacatcca cctggccatc catcaggctc
     1621 agcgcctggc gggtgagcgt gcggcattcg ttcagccctt gcccccagaa tttgcgcgct
     1681 tcccagtagc ggtcgtagct ggccgaattg cgaaacccca aaaaaatcgc cagggcaatg
     1741 cccaccaggg tgaacggcat cggggtgagc ggggttttcc agcccagcac cgtgccgtga
     1801 ctggtggtga tgatggttgc caaggcggtg accaggccca gttgcggcag gatgctgggc
     1861 agcaccgagc cgcgccgcgc aaacaaaatg gccagccagt gcgggcgggg gcgaataatc
     1921 atgactttgg cttgaaaaaa ataaaacgcg cagtgtaagc ggcagggcgt gacatgctca
     1981 accgccgcag ggccagccca gttgagaaaa atcttcagcg gtcaattcgc gccattggcc
     2041 aggggccaga tccgctggca agaccagctc acccatcgcc tcgcggtgca gggcgtccac
     2101 ccggttgccg gcggcggcca ccatgcgctt gacctgatgg tatttgcctt cgccaatggt
     2161 cagcagcagg gtgtggctgt cgcgcgcggc gcaggccagc gccgccagcg gggccggctc
     2221 gtcgtgcagt tgcacgccct ggcgcaaggc ggtcagctgg gtgtcgtcca gcggatggcg
     2281 cacgctgacg gcgtaggttt tgggcacatg ccggcgcggc gaggtgaagt gatgaataaa
     2341 ctggccgtcg tccgacagca gcagcaagcc ggtggtgtcg gcatccagcc ggcccacggg
     2401 ctgcacgccg cgttcgacat acggcgccgg cagcaggcgc agcaccggca ggtggtgttg
     2461 cggcgcgcgc gagcattcat agccggtggg tttgttcagc agcagataca ccttgctggc
     2521 cgcctgccag ggctgttcat ccacccagaa ggccagatcg gcgctgaccg gggttttcgg
     2581 gttcaggcat gggccagcgg ccagcgccac gcggccggcc tcaatcagcg cccggcaggc
     2641 tttgcggctg ccaaagccct ggctgtgcag cagtctttcc agttccatgg ccggcagcat
     2701 agcgcagatt ggccgcgcgt gccgagcggg tgcgctggct tggcggcgct cagggttgcg
     2761 ccagcggcag ggtggccagc cagtcggcca gcgccgccag gccttctggc caggggccaa
     2821 agccggcgtc ggtgttgata tggccggcgg caggcaacac ggtcagcgcg ctgccccatt
     2881 ggcgggccag ggcatcggcg cgggcggcgg cgcagtaggg gtcgttgtcg ctggcgatga
     2941 tctggctggc aaacggcagc ggcgcgcgcg gcagcggggc aaagccggcc acggcgggcg
     3001 gggcgtcggc gcgttcggtg tcggccgggg ccaccagcca ggcggcgacc acccgcgcag
     3061 cgctgtccgg gtgttgcgcc gcccagtggg cggtggtggc gcagcccagg ctgtgcgcca
     3121 ccaacaatac cggtcgatcg gccgcgcgca cggcggcgtc cagcgtggcc acccagtcgg
     3181 cgcggctggg cgtcagccag tcggcttgct gcacccgctg ccaggccgga tgggcgtctt
     3241 gccagcggct ttgccagtgt tcggggccag agttttgcca gccggggacg accagcacgt
     3301 ggtagcgctc aaaaatcggg gaggtcatga cttgccttta cacgggccgc ccctggcgcg
     3361 cggccgggcg gcaatcgaaa aaaccattgc cacagcatgc cagaaagccg ctggcgcgcc
     3421 catcgccgtc aggnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnntggcctg
     3541 cggccggctg tgcagcgggc ccacccagcg gccttgcagg ccatcgcagc cggcttgccc
     3601 ggccagctcg gcgtggcggg cggtgtccac cccttcggcg atcacctggc agccggcggc
     3661 gtgcaggatg tccaccgcgc gcggcaaggc gtcgcgcgcc gccgggtgcg acaaggcgcg
     3721 atgcagcagg cgttcgtgta gcttgaccgc gtccggctcc agcaaccaca cgcgcggcag
     3781 gctgagttcg gaagcgccaa agtcgtccag cgccagcttg aagccaaagc gccggtagct
     3841 gtccatgcag cgttgcaggc gcagcaaatc gacttcagcg tcttcgacaa tttccagcgt
     3901 cagctgtgag gggcgcaagc ccagctcggc aatcgcctcg gcaaaaaatt cgccatggcc
     3961 gtcgggcagg ttgatcagat gcagcgcgtg cacattcaga tgcagcgcgg cgtccggctg
     4021 gccgtggcgc acaaagttca gacagtgggt gacccggcac agccggtcca gcagcacggt
     4081 gcgcgatggc gcgcgctcga cggcaaacag cagcggcggc gccacgctgt ggccggccgg
     4141 gccgctgatg cgcagttggg ctttataggc aaacggcgtg gccgcgcctg gggccagaat
     4201 cggagcaaac tggctgctta gccgccattc gtcaaaactg gccgccactt gctgttgcgg
     4261 cgtcagccgc aggctgtcgg ccgggtggcc ggcagcgaca aagcgggcgt tgaaatgcgc
     4321 cagctgcgga tcgtggctga ccgccttgcg ccggccgggg cggccaggcg cagccggcgg
     4381 cagggccacc ggcttgggcg gcgctggcgg gcgcggcgcg tattgcgcca tcagactggg
     4441 gtcgagcagg gccagcaaag gcatgcgcgc cggccgccct gtcacgggct gcgcgccgtc
     4501 gggcagcgaa gcatcggaag aaacagacgg aacagacatg accacacgcc agagtaagag
     4561 ccaaaagaag gcttcactat acgggcgcgg ctttagttct tttaagaatc aaattgacgg
     4621 tttaaattcc aa
//
LOCUS       DAOPYX010000237         4628 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_23823_length_4628_cov_6.138640, whole genome shotgun sequence.
ACCESSION   DAOPYX010000237 DAOPYX010000000
VERSION     DAOPYX010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 4628)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4628)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4628
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23823_length_4628_cov_6.138640"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..515
                     /locus_tag="PKN55_11175"
     CDS             <1..515
                     /locus_tag="PKN55_11175"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012697047.1"
                     /note="One of the components of the high-affinity
                     ATP-driven potassium transport (or KDP) system, which
                     catalyzes the hydrolysis of ATP coupled with the exchange
                     of hydrogen and potassium ions; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit B"
                     /protein_id="HNX71849.1"
                     /translation="VAMTGDGTNDAPALAQADVAVAMNSGTQAAREAANMVDLDSSPT
                     KLIEVVEIGKQMLMTRGALTTFSLANDVAKYFAIIPAAFAATYPQLNALNVMGLSSPA
                     SALLSAVMFNALIIIALIPLALRGVRYRPLGAAVLLRRNLLLYGVGGMVLPFAGIKLI
                     DLGLSALGWV"
     gene            678..1268
                     /gene="kdpC"
                     /locus_tag="PKN55_11180"
     CDS             678..1268
                     /gene="kdpC"
                     /locus_tag="PKN55_11180"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpC"
                     /protein_id="HNX71850.1"
                     /translation="MISTLRLSPALAPGLRLLGLFTLLLGLLYPLLVYGLANALWPWQ
                     AQGSLWLRHGQPVGSALIGQSFAGPQWFHGRPSAISVEPGQTPVSTGSNLGPSQPQLL
                     TQAHERLLAWRASEQQAGPAPQDMLTASASGLDPHISLAAALAQVARVAQARGLDAAQ
                     LAAQVRAQAAHPWFSDGQAALARVNVLQLNLLLETP"
     gene            1265..3898
                     /locus_tag="PKN55_11185"
     CDS             1265..3898
                     /locus_tag="PKN55_11185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008953815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4118 domain-containing protein"
                     /protein_id="HNX71851.1"
                     /translation="MNPPAARPDPDALLAAQPRRRGRLKLFFGAAPGVGKTWAMLAEA
                     AERRAQGLDVLVGWVDTHGRADTEAMLAGLEVLPRQARAHRGAQLWEFDLDSVLARRP
                     ALVVVDELAHSNAPGSRHPKRWQDVEELLAAGIDVYSAMNVQHLDSLNDVVARVTGVA
                     VRETVPDTVFDHADEVRLVDLPPDDLLQRLAAGKVYLPEVAERAAGQFFRKGNLIALR
                     ELALRRLADRVDGQMRAQRGAAPSPVWDTSFGLLLRLNGDARDDDALRQCARLARALA
                     APWHAVWLDSGHDDGQRAHALASLSRAGELGAVTLLLSGGEPAALLAGYARRHNLSLL
                     ASSDHASALTRALARQAPELNLLWLGGAAPAPAPRWRLAWQALTPGWSGAAAAGALCA
                     AITVLALPLRGVLDATNLVMFYLLGVLWTALRHGRGPAALAAVLSVALFDFFFVQPHL
                     SFAVSDVEYLITFAVMLIVGLVAGQLVARERHSAARARQREAHTRLLYEMARELGSAL
                     LPEQVADISARALRAALDADIHLWLPDANDTLSLDAPASWEDAAIARWCHDHGEAAGQ
                     GTHTLPSAPCLYLPLQATMRTRGVMAIHAADPTRLSDPDNRRLCEAAATLAAQTLERL
                     HYIDVAQHTLVAMESERLRHSLLAALSHDLRTPLTALSGNAETLRNALARAQRPEAEQ
                     AGLLVEDSRRLARLVTNLLEMARLQAGAVTLRQDWLPVEELVGSALAALGPALDAHPL
                     TLELAADCPLVYGDPLLLERVLVNLLDNAGKYSPPGAGISLSAAREGDYTALRVRDHG
                     PGLPAGDAARLFEPFSRGERESAITGVGLGLAICRAIADAHQARLEAANAADGGACFT
                     LWLPWRDAPAAPFDGENSEHA"
     gene            3891..4577
                     /gene="kdpE"
                     /locus_tag="PKN55_11190"
     CDS             3891..4577
                     /gene="kdpE"
                     /locus_tag="PKN55_11190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012697050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
                     /protein_id="HNX71852.1"
                     /translation="MHEATLLVIEDDAAIRRFVRCALEEQGHKVHEADTLARGLIEAG
                     TRKPDVLILDLGLPDGDGVDLLRDLRSWSQTPVLVLSARVDETDKIDALDAGADDYLT
                     KPFGVGELQARVRALLRRRHGPDASPEVWLGEAVRVDLPARAVWRDGEPVHLTQLEYR
                     LLATLIAERGKVLTHRQLLKAVWGPTAVEHSHYLRIYMAHLRQKLEADPARPRFLLTA
                     TGVGYRLAAG"
BASE COUNT          668 a         1588 c         1649 g          723 t
ORIGIN      
        1 tggtggcgat gaccggcgac ggcaccaacg acgccccggc gctggcccag gccgatgtgg
       61 cggtggccat gaacagcggc acccaggccg cgcgcgaagc cgccaatatg gtggacctgg
      121 actccagccc gaccaagctg atcgaagtgg tggaaatcgg caaacagatg ctgatgacgc
      181 gcggcgcgct gaccacgttc agcctggcca acgatgtggc caagtacttt gccatcatcc
      241 ccgccgcctt tgccgccacc tatccgcagc tgaacgcgct gaacgtgatg ggactgagca
      301 gcccggcctc ggcgctgctg tcggcggtga tgttcaatgc gctgatcatc attgcgctga
      361 ttccgctggc gctgcgcggc gtgcgctacc gcccgctggg cgcagcggtg ctgctgcggc
      421 gcaacctgct gctgtacggc gtgggcggca tggtgctgcc gtttgccggc atcaagctga
      481 tcgacctggg cttaagcgcc ctgggctggg tgtaactcgt ctggtttggt gcttgggcgc
      541 caaaccaggc aacattgact tacgagccgc tcacaaaact cagcgccgcc attctggcca
      601 ggcaagtgac cgcagaccca aggccacacc acttcgccag acggatttga cgagcagctc
      661 catcaggaca cgacatcatg atttccacat tacgcctctc tcccgcgctg gcccctggcc
      721 tgcgcctgct gggcctgttc accctgctgc tcggcctgct ttatccgctg ctggtttacg
      781 gcctggccaa tgcgctgtgg ccctggcagg cgcaaggcag cctgtggctg cgccatggcc
      841 agccggtggg ctcggcgctg attggccagt cgtttgccgg gccacagtgg tttcacggcc
      901 gcccctcggc catcagcgtc gagccggggc aaacgccggt gtccaccggc agcaatctgg
      961 ggcccagcca gccacagctg ctgacacaag cccacgagcg tctgcttgcc tggcgcgcca
     1021 gcgaacaaca ggctggccca gcgccacaag atatgctcac cgcctcggcc agcgggctgg
     1081 acccgcacat cagcctggcc gccgcgctgg cgcaagtggc gcgcgtggcg caggcgcgtg
     1141 ggctggatgc cgcccaattg gccgcccagg tgcgtgcgca ggcagcacat ccgtggttta
     1201 gcgatggcca ggccgcgctg gcgcgggtga atgtgctgca gctgaatctg ctgctggaga
     1261 cgccatgaac ccgcccgccg cccgccccga cccggatgcc ctgctggcgg cgcagccgcg
     1321 ccggcgcggc cggctgaagc tgttttttgg cgcggcgccc ggcgtgggca aaacctgggc
     1381 catgctggcc gaggcggctg aacggcgcgc gcaggggctg gacgtgctgg tgggctgggt
     1441 ggacacccac ggccgcgccg acaccgaggc catgctggcc gggctggagg tgctgccgcg
     1501 gcaagcgcgc gcccatcgcg gcgcccagct gtgggaattc gacctggaca gcgtgctggc
     1561 gcgccggccg gcgctggtgg tggtggacga attggcgcac agcaacgccc ccggcagccg
     1621 tcatcccaag cgctggcagg acgtggaaga actgctggcc gccggcattg atgtgtacag
     1681 cgcaatgaat gtccagcacc tggacagcct gaacgatgtc gtggcgcgcg tcaccggcgt
     1741 ggcggtgcgc gaaaccgtgc ccgataccgt gttcgaccac gccgacgaag tgcggctggt
     1801 ggatttgccg ccggacgatc tcttgcaacg gctggctgcc ggcaaggtgt atctgcccga
     1861 agtggccgag cgcgccgccg ggcagttttt tcgcaagggc aatctgatcg ccctgcgtga
     1921 gctggccttg cgccggctgg ccgatcgggt ggacggccag atgcgcgccc agcgcggcgc
     1981 agcgcccagc ccggtgtggg acacctcatt tggcctgttg ctgcgcctga atggcgacgc
     2041 gcgcgacgac gacgcgctgc gccagtgtgc gcggctggcg cgggccttgg ccgcgccctg
     2101 gcatgccgtg tggctggaca gcggccacga cgatggccag cgcgcccacg ccctggccag
     2161 cctgtcgcgc gccggcgagc tgggcgccgt caccctgctg ctgtcgggcg gcgagcccgc
     2221 tgcgctgctg gccggctacg cccgtcggca taatctgtcg ctgctggcct caagcgatca
     2281 cgccagcgcc ctgacccgcg cgctggcgcg ccaggcgccg gagctgaatc tgctgtggct
     2341 gggtggcgcc gcgcccgccc cagcgccacg ctggcggctg gcctggcagg cgctgacgcc
     2401 aggctggagc ggcgcggcgg ccgctggtgc gctgtgcgcc gccatcaccg tgctggcgct
     2461 gccgctgcgc ggcgtgctgg acgccaccaa tctggtgatg ttctacctgc tgggcgtgct
     2521 gtggaccgcg ctgcgccacg gccgtggccc ggcggcgctg gcggcggtgc tgtcggtggc
     2581 cttgtttgat tttttctttg tccagccgca tctgtcgttt gccgtctcgg atgtggaata
     2641 cctgatcacc tttgccgtga tgctgatcgt cggcctggtg gccggccagt tggtggcgcg
     2701 cgagcggcac agcgcggcgc gcgcccgcca gcgcgaggcg cacacccggc tgctgtacga
     2761 aatggcgcgc gagctgggct cggcgctgct gcccgagcaa gtagccgaca tcagcgcgcg
     2821 cgccttgcgg gcggcgctgg acgccgacat ccacctgtgg ctgcccgacg ccaacgacac
     2881 actcagcctg gatgccccgg cgtcgtggga agacgccgcc attgcccgct ggtgccacga
     2941 ccacggcgaa gccgccgggc agggcaccca caccctgccc agcgcccctt gcctgtacct
     3001 gcccttgcag gcgaccatgc gcacccgcgg cgtgatggcg attcacgccg ccgaccccac
     3061 ccggctgagc gacccggaca accgccgctt gtgcgaggcc gccgccacgc tggcggcgca
     3121 aacgctggag cggctgcact atatcgacgt cgcccagcac accctggtgg cgatggaatc
     3181 cgaacggctg cgccattcgc tgctggcggc gctgtcgcac gatctgcgca cgccgctgac
     3241 cgcgctgtcc ggcaatgccg aaaccctgcg caatgcgctg gcgcgcgcgc aacgccccga
     3301 agccgaacaa gccggcctgt tggtggaaga cagccggcgg ctggcgcggc tggtgaccaa
     3361 tctgctggaa atggcgcggc tgcaagccgg cgcggtgacc ttgcggcaag actggctgcc
     3421 ggtggaagaa ctggtgggca gcgcgctggc ggcgctgggg ccggcgctgg atgcgcaccc
     3481 gctgacgcta gaactggccg ccgactgccc gctggtgtac ggtgacccct tgctgctgga
     3541 gcgggtgttg gtcaacctgc tggacaacgc cggcaaatac agcccgcccg gcgccggcat
     3601 cagcctgtca gccgcgcggg aaggcgacta caccgcgctg cgcgtgcgcg accacggccc
     3661 cggcctgccg gcgggcgatg cggcgcggct gtttgagcca ttcagccgtg gcgagcgcga
     3721 atcggccatt accggcgtcg ggctggggct ggcgatttgc cgcgcgattg ccgacgccca
     3781 ccaggcgcgg ctggaagccg ccaacgccgc ggatggcggc gcgtgtttta ccctgtggct
     3841 gccctggcgc gacgcgccgg ccgccccttt tgacggcgag aacagcgaac atgcatgaag
     3901 ccaccttgct ggtgatcgag gacgacgcgg cgattcgccg ctttgtgcgc tgcgcgctgg
     3961 aagaacaggg ccacaaagtg cacgaggccg acaccctggc gcgcgggctg atcgaagccg
     4021 gcacgcgcaa acccgatgtg ctgattctgg atctgggcct gccggacggc gatggcgtgg
     4081 atttgctgcg cgatttgcgc agctggagcc agacgccggt gttggtgctg tcggcgcggg
     4141 tggacgaaac cgacaagatc gacgcgctgg acgccggcgc cgacgactat ctgaccaagc
     4201 cctttggcgt gggtgaattg caggcgcggg tgcgcgcgct gctgcgccgc cgccatggcc
     4261 ccgacgccag cccggaagtc tggctaggcg aggcggtgcg agtggatttg cccgcccgtg
     4321 cggtatggcg agacggcgag ccggtgcacc tgacccagct ggaataccga ttgctggcca
     4381 cgctgattgc cgaacgcggc aaggtgctga cccaccgcca gctgctcaaa gcagtgtggg
     4441 ggcccacagc ggtggagcac agccattatc tgcgcattta catggcgcat ctgcgccaga
     4501 agctggaggc cgacccggcg cggccgcgct ttttgctgac cgccaccggc gtgggctacc
     4561 ggctggcggc gggctgagcg cgctcaggcc gttggggtaa cctcagcagt gcaatgcggg
     4621 cagcggct
//
LOCUS       DAOPYX010000238         4622 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_23862_length_4622_cov_11.361506, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000238 DAOPYX010000000
VERSION     DAOPYX010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 4622)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4622)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4622
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23862_length_4622_cov_11.361506"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..257)
                     /locus_tag="PKN55_11195"
     CDS             complement(<1..257)
                     /locus_tag="PKN55_11195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014088416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HNX71853.1"
                     /translation="MSKEAIAARLKPVGVSVANEGGAPGSRTGEAVYKAICSSCHAAG
                     LAGSPKFADAGAWAGRISQGFDTLVKHAVEGIRGMPAKGGAT"
     gene            536..612
                     /locus_tag="PKN55_11200"
     tRNA            536..612
                     /locus_tag="PKN55_11200"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:570..572,aa:Arg,seq:ccg)
     gene            748..2616
                     /locus_tag="PKN55_11205"
     CDS             748..2616
                     /locus_tag="PKN55_11205"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM diverse intracellular signaling
                     domain-containing protein"
                     /protein_id="HNX71854.1"
                     /translation="MWRVLQNCLLYVFLLCTVAPALAGGVLRLDPQAAGQRAAHQIDL
                     LEDPSRILGLSDVQGPEYAERFLPASLVEGDINLGYSKSAYWLRLTVQAPQDHIIRNW
                     LLELGFPALDHISAYIVDKHGWRAYQTGDMLTFDQRPYPHRHFIFPMLLDPGQITTIY
                     LRVESQGTLTLPVMIWTEPALLVYSQSAYGLLALYYGMLLALALYNLLLFLSLRDPVY
                     LAYVLFTVSMAIGQISLNGLGNQYLWPDWPAWGNVALPVGFALCGAFGTWFTRLFLHT
                     RQTAPLYDRVLFGLGGIFAILAAAPLALPYQWVAVLVSLQGISFAVITTSIALFGLRA
                     GNRSARWFLLAWSLLLAGTLVMGARNLGWLPTNMLTTHAIQIGSALEMLLLSFALADR
                     IHLAREEKEKAQSETLQAKESMVSALRQSEQLLETRVIERTRELAETNARLRISEARL
                     TELAERDPLTGLANRRRLTEALYQALNQASDRHGEGALLLIDLDHFKPVNDRCGHEAG
                     DRLLQEVARRLHACAGPMDTVARLGGDEFVLLLPPPQSRSTVETLADQLLVSLRQPVD
                     TPAGRHDVSASIGIAYLPHDGHTPEELLKVADLAMYHAKDKGRDNWSRALATDTQP"
     gene            complement(2766..>4622)
                     /locus_tag="PKN55_11210"
     CDS             complement(2766..>4622)
                     /locus_tag="PKN55_11210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018152534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HNX71855.1"
                     /translation="AAITFTNKAAREMLERVGKLLKPGEGRGLTVSTFHSLGMQLLRQ
                     EARELGYKPQFSILDAADAGKIVSDIVASTDKQEIRRVQNAISRWKNALIGPQTALAQ
                     ADNEQDLTAACAYLSYQDTLFAYQAMDFDDLIRLPVELLRDKPAVLERWQNKLRYLLI
                     DEYQDTNTCQYQLVKLLTGERGMFTAVGDDDQSIYAWRGANMENLALLQKDFPRLKLI
                     KLEQNYRSTLRILRAANSVIANNPKLFEKQLWSELGHGDLLTVVQCKDEESEAETVAA
                     RLLAHKFEHRTLFSDYAVLYRGNHQARIIEQAFRNQKIPYLISGGQSFFDKTEIKDIL
                     SWLRLLVNEDDDPAFIRAITTPKRGVGAVTLEKLGAYAGERKISLFAATQEEGLRHRL
                     AEAQRAPLEAFAQFVLRYQQRASREPAGPLIVELVAGIGYEAWLFDSEEPRPAESKWK
                     NVQELMRWLGEKGERDAKNLIELTQTIALITMLEGREEGEVDAVRLSTLHASKGLEYG
                     HVFLIGCEEGILPHQESIDNEQVEEERRLMYVGITRAQRSLTISHCHKRKRAGEWAFV
                     DPSRFIDELVQEDVRYLGKPGATATVNREAGLARLNALKAAMARGKGKTSGS"
BASE COUNT          849 a         1464 c         1358 g          951 t
ORIGIN      
        1 gtggcgccgc ccttggccgg catgccacgg atgccttcca ccgcgtgctt gaccagggtg
       61 tcaaagcctt gcgagatgcg gccagcccag gcgccagcgt cggcaaactt gggcgaaccg
      121 gccaggccgg cagcgtgaca ggacgagcag atggccttgt acacggcttc gcccgtgcgg
      181 ctgcccggtg cgccaccttc gttggcgacc gacacgccca ccggcttcag gcgcgcagca
      241 atggcttctt tggacatgac ttcagcgtcc accaccatgc cactcgtaaa cagtttggcc
      301 agcagccaaa cactcagaac aaccaagaca agcacggaga ggatgacgcg aattgcgtct
      361 cccgagctac ccttatttgc gctacccatt ggccacggcc tcacatagag aaagtaaaaa
      421 aagaataatg acaatcagaa taggggaatt atacgtaaac gcctgcccct tggcaaagac
      481 ggcaactgga ctgacggcca caaatcggct atgattctca ggcttttctt cccaagcacc
      541 cgtagctcag ttggatagag tgtcagtttc cgaagctgaa ggtcagtggt tcgatcccac
      601 tcgggtgcgc catctcttgc gcggtaattt tccgccattc tgttgtatct tcaccgtatt
      661 tacacttagt ttgtttgcag attttcttca cggccagccc gagagtgcgc gatactccag
      721 agcatgaaat ggtctggaat gcgaatcatg tggcgtgtgc tgcaaaactg tctgctgtat
      781 gtgtttctgc tgtgcactgt cgctccggcc ctggctggtg gcgtgctgcg cctggatccg
      841 caagcggctg gccagcgcgc cgcccatcag atcgacctgc ttgaagatcc cagccgtatt
      901 ctggggctga gtgatgtaca aggcccggaa tacgctgagc gctttttacc ggccagcctg
      961 gtggaaggcg acatcaatct gggctactca aaaagcgcct actggctgcg gcttaccgta
     1021 caagcccctc aggatcacat catccgcaat tggctgctgg agctggggtt tcccgcgctc
     1081 gatcatatca gcgcctatat tgtggacaag catggctggc gcgcctatca gactggcgac
     1141 atgctgacct tcgaccagcg accgtatccg caccggcatt tcatctttcc catgctgctg
     1201 gacccgggcc agatcaccac catttacctg cgagtggaat cccagggcac actcaccctg
     1261 ccggtgatga tatggaccga accggcgctg ctggtataca gccagtcggc ctacggcctg
     1321 ctggcgctgt actacggcat gctgctggcg ctggcactgt acaacctgct gctgttcctt
     1381 tctctgcgtg acccggtgta tctggcttac gtgctgttta ccgtcagcat ggccattggc
     1441 cagatttcgc tcaatggtct gggcaaccag tatctatggc cagactggcc ggcctggggc
     1501 aatgtggccc tgccagtagg atttgccctg tgtggtgctt ttggcacctg gttcacccgc
     1561 ctgttcctgc atacccggca aacggcaccg ctgtatgacc gggtgttgtt tggcctgggc
     1621 ggcatttttg ccatcctggc tgcagcgcca ctggcgttgc cctatcagtg ggtggcggtg
     1681 ctggtgtccc tgcaggggat ttcgtttgcc gtgatcacca cctccattgc cctgtttggc
     1741 ctgcgcgccg gcaaccgcag cgcgcgctgg tttttgctgg cctggtcttt gttgctggcg
     1801 ggcacactgg tgatgggtgc gcgcaatctg ggctggctac ctaccaatat gttgaccacc
     1861 cacgccatcc agattggctc ggcactggaa atgctgctgc tgtcgtttgc cctggcggat
     1921 cgcatccacc tggcgcgcga ggaaaaagaa aaagcccaga gtgaaaccct gcaagccaaa
     1981 gaatccatgg tcagcgcctt gcgccaatcg gagcagttgc tggaaacccg ggtgattgaa
     2041 cgcacccgcg agctggccga aaccaatgcc cgcctgcgca tcagcgaggc gcggctgacc
     2101 gagctggccg agcgcgaccc gctgaccggc ctggccaacc gtcgccgctt gaccgaagca
     2161 ctgtatcaag cgctcaacca ggccagcgac cgccatggcg agggggcact actgctgatt
     2221 gatctggacc acttcaagcc agtcaacgac cgctgcggcc atgaagctgg cgaccggctg
     2281 ctgcaggaag tggcacgccg actgcacgcc tgcgccggcc cgatggacac cgtggcccga
     2341 ctgggcggcg acgaatttgt gctgctgctg ccgcctccgc aaagccggag cacggtggaa
     2401 acgctcgcgg accagcttct ggtcagcctg cgccagccag tagacacccc ggcaggccgc
     2461 cacgatgtca gcgccagcat cggcattgcc tacttgcccc acgatggcca cacgccagaa
     2521 gaattgctca aagttgccga cctggccatg taccacgcca aggacaaggg ccgcgacaac
     2581 tggtcgcggg cactggccac cgatacccag ccctgaagct caccacttag agccgctaac
     2641 aaaacccagc gaagcggtcc tggctaggcg tgtgaccgca gacagtacaa gtagtacgac
     2701 aaggtcacac aacaacgcca gggggttttg ttagcggctc ttagagctgt gttttgtgcg
     2761 ccacgctagc tgccactggt cttgcccttg ccgcgcgcca tcgccgcctt cagcgcattc
     2821 agccgcgcca gcccggcttc gcggttcacc gtggccgtgg cgccgggctt gcccagatag
     2881 cgcacatcct cttgaaccag ttcgtcgata aagcgcgagg ggtccacaaa cgcccactcg
     2941 ccagcgcgct tgcgcttgtg gcagtggctg atggtcaggc tgcgctgggc gcgggtgatg
     3001 cccacgtaca tcaagcggcg ttcttcttcc acctgctcgt tgtcgatgga ttcctggtgc
     3061 ggcaggatgc cctcttcgca gccaatcaaa aacacatggc cgtattccag ccccttggag
     3121 gcgtgcaggg tggacaggcg cacggcgtcc acctcgccct cttcgcggcc ttccagcatg
     3181 gtgatcaagg ctatggtctg ggtgagctcg atcaggttct tggcgtcgcg ctcgcctttt
     3241 tcgcccagcc agcgcatcag ctcctgcacg ttcttccact tgctttccgc cggacgcggc
     3301 tcttcgctgt cgaatagcca ggcctcgtag ccaatgcccg ccaccagctc gacaatcaag
     3361 ggcccggccg gctcgcggct ggcgcgctgc tggtagcgca gcacaaattg ggcaaatgct
     3421 tccagcggcg cgcgctgcgc ctcggccagc cggtggcgca ggccttcttc ctgggtggcg
     3481 gcaaatagcg agattttgcg ctcgccggca tacgccccca gtttttccag cgtcaccgcc
     3541 cccaccccgc gcttgggcgt ggtgatggcg cggataaacg ccgggtcgtc gtcttcgttc
     3601 accagcaggc gcagccagga cagaatgtcc ttgatttcgg tcttgtcaaa aaacgactgc
     3661 ccgccagaga tcaaataggg gattttctgg ttgcgaaacg cctgctcgat aatgcgcgcc
     3721 tgatggttgc cgcgatacag cacggcgtaa tccgaaaaca gcgtgcggtg ttcaaactta
     3781 tgcgccaaca gccgcgcggc gacggtttcg gcttcggatt cttcgtcctt gcactgcacc
     3841 acggtgagca aatcgccatg gcccagctcg ctccacaatt gcttctcgaa cagtttcggg
     3901 ttgttggcga tcacgctatt ggccgcgcgc aaaatgcgca gcgtcgagcg gtagttttgc
     3961 tccagcttga tcagcttgag ccggggaaag tctttttgca gcagcgccag gttttccata
     4021 ttggccccgc gccaggcgta gatcgactgg tcatcgtcgc ccacggcggt gaacatgccg
     4081 cgctcgccgg tcagcagctt gaccaactga tactggcagg tgttggtgtc ctgatattcg
     4141 tcgatcagca aatagcgcag cttgttctgc cagcgctcca gcacggcggg cttgtcgcgc
     4201 agcagctcca ccggcaggcg gatcaggtcg tcaaaatcca tcgcctggta ggcaaacagc
     4261 gtgtcctgat agctcaggta ggcgcacgcg gcggtgagat cctgctcgtt atcggcttgc
     4321 gccagcgccg tctgcgggcc gatcagcgcg tttttccagc gcgaaatggc gttttgcacc
     4381 cggcggattt cctgcttgtc ggtgctggcg acaatatccg agacaatctt gccggcgtcg
     4441 gcggcgtcca gaatggaaaa ctgcggctta tagcccagct cgcgcgcctc ttgccgcaac
     4501 agctgcatgc ccagcgagtg aaaagtggac accgtcagcc cgcggccctc gcccggcttg
     4561 agcaatttgc ccacccgctc cagcatctcg cgcgccgcct tgttggtgaa ggtgatggcg
     4621 gc
//
LOCUS       DAOPYX010000239         4602 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_24025_length_4602_cov_10.620629, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000239 DAOPYX010000000
VERSION     DAOPYX010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 4602)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4602)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4602
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24025_length_4602_cov_10.620629"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..527
                     /locus_tag="PKN55_11215"
     CDS             <1..527
                     /locus_tag="PKN55_11215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009119104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HNX71856.1"
                     /translation="AMERDAVKRIVLVRPAVEAGEKLGFLPGDLAQKVDPYLRPLYDA
                     LYDLMGFDRVSKLFEKNLIEIAPLAYMRGRTLNHAFIILDEAQNTTPEQMKMFLTRIG
                     FGSKAVITGDVTQIDLARHQKSGLIEVEKILNQVRGICFHHFQSDDVVRHPLVQKIVD
                     AYDRWQAASPDTHA"
     gene            524..1027
                     /gene="ybeY"
                     /locus_tag="PKN55_11220"
     CDS             524..1027
                     /gene="ybeY"
                     /locus_tag="PKN55_11220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012695779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HNX71857.1"
                     /translation="MKLAKTVYRPRPRQLDLDVHYAVPLIGLPSPEQFSRWVAAALRN
                     GPRLAQIGLRVVDEAEGRELNHGYRGKDAATNVLSFALNEGEDSPADLPLFGDIVLCA
                     PVVAREAAEQGKTLHAHYAHLTVHGVLHLCGHDHEDDSEAEAMEALETVILARLGYDN
                     PYAESAD"
     gene            1038..1898
                     /locus_tag="PKN55_11225"
     CDS             1038..1898
                     /locus_tag="PKN55_11225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter associated domain-containing
                     protein"
                     /protein_id="HNX71858.1"
                     /translation="MDEAGSKPSWLERLSALLMREPEDREELIELLHAAFERNLLDAD
                     ALGMIEGVLQVGELSVRDIMIPRSQMDVIRVSESVAHFLPFVLDTAHSRFPVIGRDKD
                     DVLGILLAKDLLRYFADPDAFDLRDSLRPAVFIPESKRLNVLLKEFRSTRNHMAIVVD
                     EYGGVAGLVTIEDVIEQIIGDIEDEYDFDETESNIIRDHSGRYRVKALTEIEDFNAAL
                     GVAFADDEYDTIGGLVVSRFGHLPKRGESIDHAGLRFQVIRADSRRIHSLLVERLPEA
                     DTTEQPAPAA"
     gene            1895..3379
                     /gene="lnt"
                     /locus_tag="PKN55_11230"
     CDS             1895..3379
                     /gene="lnt"
                     /locus_tag="PKN55_11230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014085766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HNX71859.1"
                     /translation="MKLPDSRASHWLLTVASGALSVLCFAPFRQFWLMPLLLALLFSL
                     ALAQPRARQAAGLGALWGLSAYTANFYWIYISLHDVGGMPAWLALPMTLLLPAYLCLY
                     PALALGLAHRLGAERAWWTLPALWTLSEWLRATVMTGFPWGNIGYSQIPEGPLAGWAP
                     VGGVYLVAAWVALSAAALAHGARHGLRRAAPAMALGVLVWLAGMGLKTVSWTEDAGEL
                     DVALLQGNIAQEMKWRPETFAHTLQTYQQLLDQYPARVVILPETAIPLFADQVPPGVL
                     AWLADSARAQGSELVTGIPYQDPQHAERYYNAVLALTSPQPVYGKDHLVPFGEFVPLP
                     WLTGWVYRYLNMPLAGFSSGGAVQAPMQLAGHRLAFNICYEDSFGDELRAGARQAEAL
                     VNLSNLAWFGHSTAADQHLQLSQTRALETGRPMLRATNTGATAVVRADGSVQARLADF
                     TTGGLRARVETRRGQTPYLRLGDLPILLASLLVLALAIWRRLRG"
     gene            3655..>4602
                     /locus_tag="PKN55_11235"
     CDS             3655..>4602
                     /locus_tag="PKN55_11235"
                     /inference="COORDINATES: protein motif:HMM:NF010613.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF475 domain-containing protein"
                     /protein_id="HNX71860.1"
                     /translation="MKHFTFSFVFTAVALALVGMWGFHLNGVSGALAAVTLAAVLAAM
                     EVSLSFDNAVVNAGVLKTMNHFWRMMFLTVGILVAVFGMRLVFPIVIVSVAADIGMVD
                     VVKMALSQPDEYAARLTAHHAEVSAFGGMFLLLVFLNYFFDDEKQTHWFHWLESRLGG
                     LGRIDAISVLVALLTLMGTLQWVEPSHQFVVLMAGVWGILTYLLVDCLAALLEQDEDS
                     GNGQVVRRAGLGAFMYLEVLDASFSFDGVIGAFAITKDIVIIMSGLGIGAMFVRSITI
                     YLVEKGTLDEYVFLEHGAHYAIGALALIMLASMKWHIPEL"
BASE COUNT          831 a         1399 c         1468 g          904 t
ORIGIN      
        1 acgccatgga gcgcgacgcg gtcaagcgca ttgtgctggt gcgcccggcg gtggaagccg
       61 gcgaaaaact gggctttctg cccggcgatc tggcgcagaa agtcgatccc tatctgcgcc
      121 cgctgtacga cgcgctgtat gacctgatgg gctttgaccg ggtgagcaag ctgtttgaaa
      181 aaaacctgat cgaaatcgcc ccgctggcgt atatgcgcgg ccgcacgctg aaccatgcct
      241 ttattatcct ggatgaggcg cagaacacca cgcccgagca gatgaagatg ttcctcaccc
      301 gcatcggctt tggctccaag gcagtgatca ccggcgacgt cacccagatc gacctggccc
      361 gccaccaaaa gagcggcctg attgaggtgg aaaaaatcct caatcaggtg cgcggcattt
      421 gttttcacca ttttcaaagc gacgacgtgg tgcgccatcc cctggtgcaa aaaatcgttg
      481 acgcttacga ccgctggcaa gccgccagcc cggataccca cgcatgaaac tcgccaagac
      541 tgtttaccgc ccccgcccgc gccagctcga tctggacgtg cattacgccg tgccgctgat
      601 cggcctgccc agccccgagc agttctcgcg ctgggtggcc gccgcgctgc gcaatggccc
      661 gcgcctggcg caaatcggcc tgcgcgtggt ggatgaggcc gaaggccgcg agctgaacca
      721 cggctatcgc ggcaaggacg ccgccaccaa tgtgctgagc tttgccctga acgaaggcga
      781 agacagcccg gccgatttgc cgctgtttgg cgacatcgtg ctgtgcgcgc cagtggtggc
      841 gcgtgaggcc gccgagcaag gcaagaccct gcacgcccat tacgcccatc tgaccgtgca
      901 tggcgtgctg catctgtgcg gccacgacca tgaggacgac agcgaggccg aagcaatgga
      961 agcgcttgaa actgtcatcc tggcaaggtt aggatacgac aacccttacg ccgaatcggc
     1021 tgactgaacc tcacactatg gacgaagctg gcagtaaacc atcctggctg gagcgcctgt
     1081 cggcgctgct gatgcgcgaa ccggaagacc gcgaagagct gatcgaactg ctgcacgcgg
     1141 cgtttgagcg caatctgctc gacgccgacg ccctgggcat gatcgagggc gtgttgcagg
     1201 tgggcgaatt atcggtgcgc gacatcatga ttccgcgcag ccagatggat gtgatccgcg
     1261 tcagcgaatc ggtggcgcat ttcctgccct tcgtgctgga caccgcgcat tcgcgctttc
     1321 cggtgattgg ccgcgacaag gacgatgtgc tgggcatcct gctggccaaa gacctgctgc
     1381 gctactttgc cgacccggac gcctttgacc tgcgcgacag cctgcgcccg gcggtgttca
     1441 tccccgaatc caaacgcctg aacgtgctgc tgaaagaatt ccgcagcacg cgcaaccaca
     1501 tggccatcgt ggtggacgaa tacggcggcg tggccgggct ggtgaccatc gaagacgtga
     1561 tcgagcaaat catcggcgac atcgaagacg aatacgactt tgacgaaacc gagagcaata
     1621 tcatccgcga ccacagcggc cgctaccggg tgaaggcgct gaccgagatc gaagacttca
     1681 acgccgcgct gggcgtggcc tttgccgatg acgaatacga cactatcggc gggctggtgg
     1741 tgtcgcgctt tggccatctg cccaagcgcg gcgagagcat cgaccacgcc ggcctgcgct
     1801 ttcaggtgat ccgcgccgac agccggcgca ttcactccct gctggtcgaa cgcctgcctg
     1861 aggcagacac gaccgagcaa ccagcccctg ccgcatgaaa ctccctgatt cgcgcgccag
     1921 ccactggctg ctgaccgtcg ccagcggcgc gctcagcgtg ctgtgctttg ccccattccg
     1981 ccagttctgg ttgatgccgc tattgctggc gctgctgttc agtctggcgc tggcgcaacc
     2041 gcgcgcgcgc caggccgccg gcttgggcgc gctgtggggg ctgtccgcct acacggccaa
     2101 tttctactgg atttatatca gcctgcacga cgtgggcggc atgcccgcct ggctggcgct
     2161 gccgatgacg ctgctgctgc cggcttatct ctgcctgtac cctgccttgg cgctgggact
     2221 ggctcaccgg ctgggtgctg aacgggcgtg gtggaccttg ccagcgttgt ggacactgag
     2281 cgaatggctg cgcgcgacgg tgatgaccgg ctttccctgg ggcaatatcg gctacagcca
     2341 gattccggaa ggcccgctgg ccggctgggc gccagtgggt ggggtatatc tggtggcggc
     2401 ctgggtggct ttgagcgctg ccgcgctggc gcacggcgcg cgccatggcc tgcgccgcgc
     2461 cgcgccggcc atggcgctgg gcgtattggt ctggctggcg ggcatgggcc tgaaaacagt
     2521 atcctggacc gaggatgcag gggagctgga cgtcgcactg ctacaaggca atatcgccca
     2581 ggaaatgaaa tggcggccag aaacttttgc ccacaccttg cagacctatc aacagctgct
     2641 tgatcaatat cccgcccggg tggtgattct gccggaaacc gccattccgc tgtttgccga
     2701 tcaggttccg cctggtgtgc tggcctggct ggccgacagc gcgcgcgcgc agggcagcga
     2761 gttggtgacc ggcattccct accaggatcc gcaacacgca gaacggtatt acaacgccgt
     2821 gctggcgctg acctcgccac agccagtata cggcaaggat catctggtgc catttggcga
     2881 atttgtaccg ctcccctggc tgaccggctg ggtatatcgc tacctgaaca tgccactggc
     2941 aggttttagc tcgggcggtg cagtgcaagc acccatgcag ttggccggcc accggctggc
     3001 gttcaacatc tgctatgaag acagctttgg cgatgagctg cgcgcaggtg cgcggcaagc
     3061 agaagccttg gtgaacctga gcaacctggc ctggtttggt cattccaccg ccgccgacca
     3121 gcatttgcag ctgtcgcaaa cccgtgcact ggaaaccggc cgcccgatgc tacgcgccac
     3181 caacaccggc gccaccgccg tggtgcgcgc cgatggcagc gtgcaggcgc gactggcgga
     3241 cttcaccacg ggcggcttgc gcgcccgagt ggaaacccgg cgcgggcaga caccctacct
     3301 gcgcctgggc gatctaccta tcttgctggc cagtttgctg gtgttggcat tggccatctg
     3361 gcgacgcctg cgcggctaag cgtcgctcac aaaacccagc gcagcgctct tggccaggca
     3421 cgcagccaca gacatgcagt catacagcaa ggctgcgcaa cgacgccaga gaagttttgt
     3481 gagcaacacc aagctgactt tcctgacatc agcatcagcc aacatccata ggttcaagct
     3541 cacggtgtag cacctggtac accaggcaac acaccgctct ttatgactgc cgccagccat
     3601 ctgatggcgg cggcgttgtt ctcccttgcc ccgggagttt tattcaggag catcatgaag
     3661 cattttactt tttcttttgt gttcaccgcc gtggcgctgg ccttggtggg catgtgggga
     3721 tttcatctga atggcgtcag cggcgccttg gcggcggtga cgctggcggc ggtgctggct
     3781 gccatggagg tgtcgctgtc gtttgacaac gccgtggtga acgcaggcgt gctgaaaacc
     3841 atgaaccact tctggcggat gatgttcctg accgtgggca ttctggtggc ggtgttcggc
     3901 atgcgcctgg tgttcccgat cgtgattgtg tcagtggcgg cggacatcgg catggtggac
     3961 gtggtgaaaa tggcgctctc gcagccagac gaatacgcgg cgcggctgac cgcccaccat
     4021 gccgaagtct cggcatttgg cggcatgttc ctgctgctgg tgtttctgaa ttactttttt
     4081 gatgacgaaa aacaaaccca ttggtttcac tggctggaaa gccggctggg cgggctgggg
     4141 cggattgacg ccatcagcgt gctggtggcg ttgctgacgc tgatgggcac gctgcaatgg
     4201 gttgagccca gccatcagtt tgtggtgttg atggccggcg tatgggggat tttgacctat
     4261 ttgctggtgg attgcctggc ggcgctgctg gaacaagacg aagacagcgg caatggccag
     4321 gtggtgcgcc gcgccgggct gggggcgttc atgtatctgg aagtgctgga cgcttcgttc
     4381 tcgtttgacg gcgtgattgg cgcgttcgcc atcaccaaag acatcgtgat catcatgtcc
     4441 ggcctgggca ttggcgccat gttcgtgcgt tcgatcacca tttatctggt ggaaaaaggc
     4501 acgctggacg agtatgtgtt tctggagcac ggcgcccact acgcgattgg cgcgctggcg
     4561 ctgatcatgc tggcgtcgat gaagtggcac atccccgagc tg
//
LOCUS       DAOPYX010000240         4602 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_24026_length_4602_cov_7.133275, whole genome shotgun sequence.
ACCESSION   DAOPYX010000240 DAOPYX010000000
VERSION     DAOPYX010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 4602)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4602)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4602
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24026_length_4602_cov_7.133275"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..533
                     /locus_tag="PKN55_11240"
     CDS             <1..533
                     /locus_tag="PKN55_11240"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNX71861.1"
                     /translation="RAAGVLAGIREFARKRARVREPHALAKLTERSLSLFRGMLAQAP
                     AITWQCSAEAADALVLADPLQIQQVLLNLLKNACDAQQAAGREQMPIVVSLQRDGARL
                     ALAVRDHGDGLDPEAQRRLFEPFFTTKPDGLGLGLSICKTIAEAHGGELSADTPADGP
                     GLCLWLRLPCLENPPA"
     gene            530..1177
                     /locus_tag="PKN55_11245"
     CDS             530..1177
                     /locus_tag="PKN55_11245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012695695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNX71862.1"
                     /translation="MNTAPHVHVIDDEAPFLRSVLFLLESAGWPASGHDSAEAFLAAN
                     PPFPPGGGCLVADIRMPRVSGLELQRRLQAAGSPFPLIFVTGHGDVELAVEAMKAGAV
                     DFLQKPFRDQPFLDAIERAVELSRAHHAASQRSQEARAILTRLSPRETEVARWLALGW
                     ANKAIARQLAISEHTVHVHRQHIMDKTDAGSPADLARLILRADPAGLDEAGMAAD"
     gene            complement(1400..3448)
                     /locus_tag="PKN55_11250"
     CDS             complement(1400..3448)
                     /locus_tag="PKN55_11250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HNX71863.1"
                     /translation="MKPVSLVRHHVRPLLVGALLWLGAGAAALAAPASADIQRGEYLA
                     RLGDCVACHTAERGQPMAGGRALQTPFGTLYSTNITPDRATGIGQYSFAQFDRAMRQG
                     VAADGHNLYPAMPYPSYAKMTADDMQALYTYLMQGLKPVSQVNKPTDMRWPFSLRWGL
                     SLWNWAFLDATPFTPDPKQDPVWNRGAYLVQGLGHCGACHTPRGIGFQEKAMTELGGK
                     GKYFLAGETVESWRALSLRGLWTVDDTALFLKTGQNRFATASGSMAEVIHHSTQHVRD
                     DDLRAIATYLKALPPGENELPAPSKPRAALTPHTPDTLYTSRGGLAYAQFCGDCHRAD
                     GGGVNRIFPPLAGNPTVSAKDPATLLHIILTGGQTAATAAHPRVFAMPGFARLSDQEL
                     AEILSFVRTSWGHGAPAVSSGDVQRMRATLDPKTTDNSRFDTPRVADMLKQPNASQLV
                     RGMRLHMETRDLLPRHVGNQLNCASCHLNAGTVADGSPFVGVSAFFPSYAPRAGRIIS
                     LEDRINGCFRRSMNGKPLPVDSADMKAMVAYFDWMKGATKPEDKVPGRGVGKIDRTLK
                     ADPVNGKQIYAQQCAVCHGDNGEGLKRADGRMIYPPLWGEQSFNIGAGMARTYTAAAF
                     VKRNMPIGFHGKFPLGQGGLSDQEALDVAEYFSHQPRPDFPDKVKDWPKDKKPDDARY
                     "
     gene            complement(3672..4553)
                     /locus_tag="PKN55_11255"
     CDS             complement(3672..4553)
                     /locus_tag="PKN55_11255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012698020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HNX71864.1"
                     /translation="MRSIGKLVIIGVGLIGGSFALAMKRAGLVGQVVGVGRSLDNLNR
                     AIELGVIDAASQDPAQAVAGADLVMVATPVGQMGRVFDAIAPVLPAQAIVTDAGSTKG
                     DVAQLMRAHLPEHLAYCVPAHPIAGSDRSGAAAAQYGLFEQRRVVLTPLEATLPEAAE
                     AVGQWWQASGARVFSMRADEHDAVFASVSHLPHLLAFAYVEMVANKTDAARCFDFAAT
                     GFKDFTRIAGSHPEMWRDISLANRDALLADLRAYQAGLAQLTQWVEAGDGAALEQMFQ
                     HAREARVQWAQRFAQGV"
BASE COUNT          839 a         1527 c         1436 g          800 t
ORIGIN      
        1 aacgcgccgc cggcgtgctg gccggcatcc gcgaatttgc ccgcaagcgc gcgcgagtgc
       61 gcgagcccca tgcactggcc aaactgaccg agcggtcact cagcctgttt cgcggcatgc
      121 tggcgcaagc gccggccatc acctggcagt gcagcgccga ggcggctgac gcactggtat
      181 tggccgaccc cttgcagatt caacaagtgc tgctcaacct gctgaaaaac gcctgcgacg
      241 cccagcaggc ggcgggccgt gagcaaatgc cgattgttgt cagcctacag cgtgatggcg
      301 cccgactggc actggcggtg cgtgaccatg gcgatggcct ggaccccgag gcgcagaggc
      361 ggttgttcga accgtttttc accaccaagc ccgatggcct tggcctgggt ttgtcgattt
      421 gcaaaaccat tgccgaagcg cacggcggcg agctcagcgc cgacaccccg gccgacggcc
      481 ccggtctgtg cctgtggctg cgcctgcctt gcctggagaa tccccctgca tgaacaccgc
      541 cccccatgtg catgtgattg acgatgaagc gccatttttg cgctcggtgc tgtttctgct
      601 ggaatccgct ggctggccag ccagcggcca tgacagcgcc gaggcgtttc tggccgccaa
      661 cccgcctttt ccgcctggcg gtggctgtct ggtcgccgat atccgcatgc cgcgtgtcag
      721 cggcctggaa ttacaacgcc gtttgcaagc cgcaggatcg ccgtttccgc tgatttttgt
      781 caccggccac ggcgatgtcg agctggccgt cgaggccatg aaggccgggg cggtggattt
      841 tctacaaaaa ccctttcgcg accagccctt tctggacgcc atcgaacgcg cggtggaact
      901 cagccgcgcc caccacgccg ccagccagcg cagccaggaa gcccgcgcca tcctcacccg
      961 cttgtcaccg cgcgaaaccg aggtggcgcg ctggctggcg ctgggctggg ccaacaaggc
     1021 cattgcccgg caactggcca tcagcgagca caccgtacat gtgcaccgcc agcacatcat
     1081 ggataaaacc gacgccggca gcccggccga tctggcccgg ctgatcctgc gcgctgaccc
     1141 ggccgggctg gatgaagcgg gcatggcggc ggactgagaa ccgctaacaa gaccctcctg
     1201 acgttgttgt gtgcctttgt cgtacatttg tactgtctac ggccacacac ctggtcagga
     1261 tcgcttcgct gagtgttgtt agcgacctaa gcgctggcga attggcctgc atcgcagaga
     1321 tcttcccagc agacgccaca ccgccacgcc ccgcatagac ggggcgtggc ggcatcacct
     1381 caagggttcg atcagcaact caataacgcg cgtcatccgg ctttttgtcc ttcggccagt
     1441 ccttgacctt gtccgggaag tccgggcgcg gctggtggct gaaatattcg gccacatcca
     1501 gcgcttcctg atcgctcaag ccaccctgac ccagcgggaa cttgccatga aagccgatcg
     1561 gcatattgcg tttgacaaag gcggcagcgg tgtaggtgcg cgccatgccg gcgccgatat
     1621 tgaacgattg ctcgccccac agcggcgggt agatcatgcg gccatcggca cgcttgagcc
     1681 cttcgccatt gtcaccatgg cacaccgcac attgctgggc gtaaatctgc ttgccgttca
     1741 ccgggtcggc cttgagcgtg cggtcgatct tgcccacccc gcgcccgggc accttgtctt
     1801 ccggtttggt cgcacccttc atccagtcaa agtaggcgac catggctttc atgtcggcag
     1861 agtccactgg cagcggcttg ccattcatcg agcgcctgaa gcagccattg atgcgatctt
     1921 ccaggctgat gatccggccg gcgcgtggcg catagctggg gaagaatgcc gatacaccaa
     1981 caaacggaga gccatcggcc acggtgccgg cattcaggtg acagctggcg cagttgagct
     2041 ggttgccaac atggcggggc agcaggtcgc gggtttccat gtgcaggcgc atgccgcgca
     2101 ccagctggct ggcattgggc tgcttgagca tatcggccac gcgcggggtg tcaaagcgcg
     2161 aattgtcagt ggttttcggg tcgagcgtgg cgcgcatgcg ctgcacatcg ccggagctga
     2221 ctgccggtgc cccatggccc cagctggtgc ggacaaaact caggatttcc gccagctcct
     2281 gatcactgag ccgggcaaaa ccgggcatgg caaacacccg tggatgcgca gccgtggcgg
     2341 cagtctgccc gccggtcagg atgatgtgca gcagggtggc cgggtctttg gcgctgactg
     2401 tcgggttgcc ggccagcggc gggaagatcc ggttgacgcc gccaccgtcc gcccggtggc
     2461 aatccccgca gaactgggcg taggccaggc cgccacggct ggtatacagc gtatcgggtg
     2521 tgtgcggcgt cagggcggcg cgtggcttgc ttggcgcagg caattcgttt tcgcctggcg
     2581 gcagcgcctt cagataggtg gcgatggcgc gcagatcatc gtcgcgcaca tgctgggtgc
     2641 tgtggtgaat cacctcggcc atcgaacccg aggccgtggc aaagcggttt tgcccggttt
     2701 tcagaaacag ggcggtatcg tctaccgtcc acagcccgcg caagctgagt gcccgccagg
     2761 attccaccgt ctcaccagcc agaaaatact tgcccttgcc acccagctcg gtcatggctt
     2821 tttcctgaaa gccaatgcca cgcggggtat gacaggcacc gcaatggccc agcccctgca
     2881 ccagatacgc gccccggttc cacacgggat cctgttttgg gtcaggggtg aacggcgtgg
     2941 catccagaaa agcccagttc cacagcgaca gcccccagcg caggctgaag ggccagcgca
     3001 tgtcagtggg tttattcacc tgactgacgg gcttgaggcc ctgcatcaga taggtgtaca
     3061 gcgcctgcat atcgtcagca gtcatcttgg cgtaagacgg ataaggcatg gccggataca
     3121 gattgtggcc atcggcggcc accccctgac gcatggcgcg atcgaactgg gcaaagctgt
     3181 actggccaat gccggtggcg cggtccgggg taatattggt ggaatacagc gtgccgaacg
     3241 gtgtttgcag ggcgcggccg ccagccatcg gctggccgcg ctcggcagtg tgacaggcga
     3301 cgcaatcgcc cagccgggcc aggtattcgc cgcgctggat atcagcactg gcgggcgcgg
     3361 ccagcgcggc ggcgccagct cccagccaga gcaatgcgcc cactagcagc ggacgcacat
     3421 gatggcggac aagagaaaca ggtttcatca gacaactccc gatacagact gcatgagaga
     3481 gcagtctagg gagcagcgcc gacaacgcct attcaatgct tggattaaac cacttaccta
     3541 aaggggttat tgccaatagc acagcgatgt gcatgtggct tggcgtcgct cacaaaaccc
     3601 cgcgaagtgt tcctgccagg cgtgcggcgc agataacggc aaggccgtgc aacgacgcca
     3661 ggaaggcgcg ctcagacgcc ctgggcaaag cgctgggccc actgcacccg cgcctcgcgg
     3721 gcatgctgaa acatctgctc cagcgccgcg ccgtcgccgg cctccaccca ttgcgtcagc
     3781 tgcgccagcc cggcttgata ggcgcgcaaa tcggccaata gcgcgtcgcg gttggccagg
     3841 ctgatgtcgc gccacatctc cgggtggctg ccggcaatgc gggtgaagtc tttaaagccg
     3901 gtggcggcaa agtcaaaaca gcgcgcggcg tcggttttat tggccaccat ctcgacatag
     3961 gcaaacgcca gcaaatgcgg caaatggctg acgctggcga acaccgcgtc gtgctcgtcg
     4021 gcgcgcatgc tgaacacccg cgcaccgctg gcctgccacc actggcccac cgcctcggcc
     4081 gcctcgggca aggtggcctc cagcggggtg agcaccaccc ggcgctgctc gaacagccca
     4141 tactgcgccg ccgccgcgcc cgagcggtcc gaaccggcaa tcgggtgcgc cggcacgcaa
     4201 taggccagat gctcgggcaa gtgcgcgcgc atcaactgcg ccacatcgcc cttggtgctg
     4261 cctgcgtcgg tgacaatcgc ctgcgctggc aacaccgggg cgatggcgtc aaacacccgc
     4321 cccatctgcc ccaccggcgt ggccaccatc accaaatccg cgccagccac cgcctgggcc
     4381 gggtcctggc tggcggcgtc gatcaccccc agctcgatgg cgcggttgag gttgtccagc
     4441 gaacgcccca cccccaccac ctgcccaacc aagcccgcgc gcttcatcgc caacgcaaaa
     4501 gagccgccga tcaagccgac gccgatgatc accagtttac cgatgctgcg caccgcgctt
     4561 ctccttcaaa aacactattt gtttaccgat gccgacgcct gc
//
LOCUS       DAOPYX010000241         4592 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_24115_length_4592_cov_9.185144, whole genome shotgun sequence.
ACCESSION   DAOPYX010000241 DAOPYX010000000
VERSION     DAOPYX010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 4592)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4592)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4592
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24115_length_4592_cov_9.185144"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(52..978)
                     /locus_tag="PKN55_11260"
     CDS             complement(52..978)
                     /locus_tag="PKN55_11260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019919698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HNX71865.1"
                     /translation="MTLNDAPGKEIVLVVDDFPENLALLYDALDEAGYTVLVATDGES
                     ALSRARLSLPDVILLDAVMPGMDGFEVARCLKADFATQHIPVVFMTGLTETEHVVAAF
                     AAGGADYVTKPLRPQEVLARIAAHMHNARQMKQARTALDAFGQATLAIAAASGKLVWQ
                     TPLARDILHRHFAEPGDYAPERLLAWTRDAERARRAGQEAPALVHTQQGCRLLASFHD
                     QTGDEEWLVVLREENDDSTLEALISAFRLTRREAEVLHWLTLGKTNRDIADILGGSPR
                     TVNKHLEHIFEKLGVETRTAAATLAVNRLRGG"
     gene            complement(965..4363)
                     /locus_tag="PKN55_11265"
     CDS             complement(965..4363)
                     /locus_tag="PKN55_11265"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNX71866.1"
                     /translation="MADAPHSPPTQRIVKVRRDYNSWVARETLEDYALRFTPRSFRKW
                     SVWRVAQTAFGGAASFLVLEAIGATMLVQAGFVNAFWAILATGLIIALAGWPISVYAA
                     RFGVDMDLLTRGAGFGYIGSTITSLIYASFTFIFFALEAAIMAYALEMSLGLPPSWGY
                     LLCALVVIPLVMHGITAISRVQLWTLPLWLALLVLPYAVVLTRYPQALAGLWAYGGEG
                     GFPAGFDLQRFGAALTVGVALITQMGEQADYLRFMPAAEGARKRRWWLAVALGGPGWV
                     LPGVAKMLGGALLAWLLLREGMAPERAVDPNQMYLAAFAEVFHSPALAVWVTALFVIL
                     SQLKINVTNAYAGSLAWSNFFSRLTHSHPGRVVWMVFNILIALLLMELEVFQAIGQVL
                     GLYSNIAISWFAAVVADLVINKPLGLSPAGIEFRRAHLYDINPVGVGAMGLASVLSVA
                     CFIGAFGPTAQPFAPLIAMLTALLAAPLIAWRTQGRYYLARPEVQQRRGRQYCVVCEQ
                     RYEPEEMSHCPAYQGPICSLCCSLDVRCDDACKPHARLASQWHALLARVLPAQVLPLL
                     ARGLGHYLLLMLGLGLTLALLMGMLYYLEWHNLPPGASPAALQASFVRIFAALWLVGG
                     ILAWWMALTHKSRQVAQEESNRQTGLLLQEIESHQRTDEQLQKARQAAEQANQAKSRY
                     ISAISHELRTPLNSILGYAQILDGDESIPANRRQAVQVIRRSGDHLLSIIDSTIDVAR
                     IESGKLTLDIRPLAFPDFISQIVSMVELQARNKGLRFDYQPEGKLPMGVRADEKRLRQ
                     ILLNLLGNAVKFTDHGSVSLKLSYRREMAVFEIHDTGPGIAADELARLFEPFERGRLA
                     RGGGSGLGLTISKMLTDLMGGELTVSSEPGQGSVFCARLYLPELRDASPGKPALLEQR
                     IGYMGVRRRILIVDNEKIDRELLVSVLAPLGFELFQAESGAECLAALPHVRPHLLFMD
                     LAMPGMNGWETLRRIREQQLTDARIAIISANAFERGQDNEVGIPPEDFILKPILVEEL
                     LDWIGRALALEWVSAPRPRSEPPPPAAPLRYPPAHWLRQLDEGVNLGYMRGIRRTLDE
                     LASEAPHCEEFIRVASEHVRHFRLDALKAMLRHGLERTAPHDPE"
BASE COUNT          900 a         1532 c         1434 g          726 t
ORIGIN      
        1 ttagcgcggc gctcactggc gagaaggcgt cagctgatgg acgccagcgc ctcacccgcc
       61 ccgcagccga ttcaccgcca gcgttgccgc cgccgtgcgg gtttccaccc cgagcttttc
      121 aaaaatatgc tccaggtgtt tgttcaccgt gcgtgggctg ccgccgagga tgtcggcgat
      181 atcccgattg gttttgccca gcgtcagcca gtgcagcact tccgcttcgc ggcgggtgag
      241 gcgaaaggcg ctgatcaggg cttccagggt gctgtcgtcg ttttcttcgc gcagcaccac
      301 cagccattct tcgtcgccgg tctggtcgtg aaaagacgcc agcaggcggc agccttgctg
      361 ggtgtgcacc agggcggggg cttcctggcc ggcgcggcgg gcgcgctcgg cgtcgcgggt
      421 ccaggccagc aggcgctcgg gggcgtagtc gccgggttcg gcaaagtggc ggtgcaggat
      481 gtcgcgcgcc aacggggttt gccacaccag cttgccgctg gcggcggcga tggcgagggt
      541 ggcctggcca aaggcgtcca gcgcggtgcg cgcctgtttc atctgccggg cgttgtgcat
      601 atgggcggcg atgcgggcca gcacctcttg cgggcgcagc ggtttggtca cgtagtcggc
      661 gccgccggcg gcaaaggcgg ccaccacgtg ctcggtttcg gtcagcccgg tcatgaacac
      721 caccgggatg tgctgggtgg caaagtcggc tttcaggcag cgggccacct caaaaccatc
      781 catgccgggc attaccgcat cgagcaaaat cacgtcgggc agcgacagac gggcgcggct
      841 gagcgcggat tcgccgtcgg tggccaccag cacggtgtaa ccggcttcgt ccagcgcgtc
      901 gtacagcagg gcgagatttt ccgggaagtc gtccaccacc aggacgattt ccttgccggg
      961 ggcgtcattc agggtcatgc ggggcggtcc tttccaggcc gtggcgcagc atggctttca
     1021 gcgcgtccag ccggaagtgg cggacatgct cgctggcgac acggatgaat tcttcacaat
     1081 gcggggcttc gctggccaat tcgtccagcg tgcggcggat gccgcgcata tagcccaggt
     1141 tgacgccttc gtccagttgg cgcagccaat gggcgggcgg gtagcgcagc ggcgcggcgg
     1201 gcggcggcgg ctcgctgcgc gggcgcggcg cgctcaccca ttccagcgcc agcgcgcggc
     1261 caatccagtc gagcagttct tccaccagaa tcggcttgag gatgaaatcc tcgggtggaa
     1321 tgcccacttc gttatcctgg ccgcgctcga aggcgttggc cgagataatg gcgatgcgcg
     1381 cgtcggtgag ctgttgctcg cggatgcggc gcagggtttc ccagccattc atgccgggca
     1441 tggccaggtc catgaacaat aaatgcgggc gcacatgcgg cagcgcggcc aggcattcgg
     1501 cgccggattc ggcctgaaac agctcaaagc ccagcggggc cagcacgctg accagcagct
     1561 cgcggtcgat tttttcgtta tcgacaatca aaatgcgccg acgcacgccc atatagccga
     1621 tgcgctgctc cagcaaggcc ggcttgccgg ggctggcgtc gcgcagctcg ggcaggtaca
     1681 gccgggcgca aaatacgctg ccctggccag gctcgctgct gacggtcagc tcgccgccca
     1741 tcaggtcggt gagcatcttg ctgatggtca gccccaggcc cgagccgccg ccgcgcgcca
     1801 gccggccgcg ctcgaacggc tcgaacagcc gcgccagctc gtcggcagca atgccggggc
     1861 cggtgtcgtg gatttcaaac accgccattt ctcgccggta gctgagcttg aggctgacgc
     1921 tgccatggtc ggtgaatttc accgcattgc ccagcagatt cagcaggatt tgccgcaggc
     1981 gtttttcgtc ggcgcgcacc cccatcggca gcttgccctc gggctggtag tcaaagcgca
     2041 agcccttgtt gcgcgcttgc agctcgacca tgctgacaat ctggctgatg aaatccggga
     2101 aggccagcgg gcggatgtcc agggtgagct tgccgctttc gatgcgcgcc acgtcaatgg
     2161 tgctgtcgat gatggacagc aaatggtcgc cgctgcggcg gatcacctgc accgcctggc
     2221 ggcggttggc cgggatgctt tcgtcgccgt ccagaatctg cgcatagccc agaatgctgt
     2281 tcagcggcgt gcgcaactcg tggctgatgg ccgagatata gcggcttttg gcctggttgg
     2341 cctgctcggc ggcctggcgg gctttttgca gttgctcgtc ggtgcgctgg tgcgattcga
     2401 tttcctgcaa taacagcccg gtctggcggt tggattcttc ttgcgccact tgccggcttt
     2461 tgtgggtcag cgccatccac caggccagaa tgccgcccac cagccacagc gcggcaaaaa
     2521 tgcgcacaaa gctggcttgc agcgccgccg ggctggcgcc gggcggcagg ttatgccatt
     2581 ccaggtaata cagcatgccc atcagcaagg ccagcgtcag ccccaggccc agcatcagca
     2641 gcagataatg ccccaagccg cgcgccagta agggcagcac ctgcgccggc aacacccgcg
     2701 ccagcagcgc gtgccactgg ctggccagcc gggcgtgcgg tttgcaggcg tcgtcgcagc
     2761 gcacatccag cgagcagcac agcgagcaaa tcggcccctg ataagccgga caatggctca
     2821 tttcttcggg ttcgtagcgc tgctcgcaca ccacgcaata ctgacggccg cgccgctgct
     2881 gcacctctgg ccgcgccagg taatagcggc cctgggtgcg ccaggcaatc agcggcgccg
     2941 ccagcagcgc ggtgagcatg gcgatcagcg gggcaaacgg ctgcgcggtc gggccaaagg
     3001 cgccgataaa acacgccacc gacagcaccg aggccaaacc catcgccccc acccccaccg
     3061 ggttgatgtc gtacaaatgg gcgcggcgaa actcaatgcc ggccgggctc agccccaggg
     3121 gtttgttgat caccaaatcc gccaccaccg ccgcaaacca ggaaatggcg atattggaat
     3181 acagccccag cacctggcca atggcctgga acacctccag ctccatcagc aacagggcaa
     3241 tcaggatatt gaacaccatc cacaccaccc ggccggggtg gctatgggtc agccgcgaga
     3301 aaaaattcga ccacgccagc gagccggcat aggcattggt gacgttgatt ttcaactgcg
     3361 acaaaatcac aaacagcgcg gtcacccaca ccgccagcgc cggcgaatga aacacctcgg
     3421 caaacgccgc cagatacatt tgattggggt ccaccgcccg ctccggcgcc atgccctcgc
     3481 gcaacaacaa ccaggccagc aaagcgccgc ccagcatctt ggccaccccc ggcaacaccc
     3541 atcccggccc gcccaacgcc accgccagcc accaacgccg cttgcgcgcc ccctccgccg
     3601 ccggcataaa gcgcaaataa tccgcctgct cgcccatctg ggtgatcaag gccaccccca
     3661 ccgtcaaggc tgcgccaaag cgctgcaaat caaacccggc cggaaaccca ccctcgccgc
     3721 catacgccca caaaccagcc agcgcctgcg gatagcgggt caacaccacc gcgtaaggca
     3781 gcaccagcaa agccagccac agcggcaaag tccacaactg cacccggctg atggcggtga
     3841 tgccgtgcat caccaacgga atcaccacca aggcgcacag caaatacccc cacgacggcg
     3901 gcaaacccag gctcatctcc agcgcataag ccatgatcgc cgcttccagg gcaaaaaaga
     3961 taaaggtgaa cgaggcgtaa atcagcgaag taatggtcga gccgatataa ccaaacccgg
     4021 cgccccgagt gagcaaatcc atgtccacgc caaaccgtgc ggcatacaca ctgatcggcc
     4081 accccgccag cgcgatgatc aagcccgtgg caagaatcgc ccagaacgca ttgacgaacc
     4141 ccgcctgcac caacatggtc gcgccaatcg cctccaacac caaaaacgac gccgcccccc
     4201 caaacgccgt ctgcgccacc cgccacaccg accacttacg aaaactgcgc ggagtaaaac
     4261 gcaacgcgta atcctccaac gtctcccgcg ccacccaact gttgtaatcc cgacgcacct
     4321 tgacaatacg ctgcgtgggc gggctgtgcg gcgcatcggc catgggcaag actccggaaa
     4381 gacagagggg gaaatcgggc gggtggttgg agcggaacag agtaagtcta ggcaggtgag
     4441 cggtgatcag cggtgcgcag caagtgtcaa ggcagagcgc ggtggcaagg tctggaattc
     4501 tcgcctgtac gggacgggtc gggtggaaag gttaggcggc atcaaaataa aaacgacgcc
     4561 aagccgcctg actattcaaa gcgctgtcgg tt
//
LOCUS       DAOPYX010000242         4575 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_24241_length_4575_cov_9.892699, whole genome shotgun sequence.
ACCESSION   DAOPYX010000242 DAOPYX010000000
VERSION     DAOPYX010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 4575)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4575)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4575
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24241_length_4575_cov_9.892699"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(57..1019)
                     /locus_tag="PKN55_11270"
     CDS             complement(57..1019)
                     /locus_tag="PKN55_11270"
                     /inference="COORDINATES: protein motif:HMM:NF012708.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HNX71867.1"
                     /translation="MAVLHNKHLRRRVMLRPVHTFGRHPASSRTVLQEADVSKLHAVV
                     RWNGALWEICDQSRNGTYVSGDKLQPGQWRVLQMGDVIRFAASQQAQWTVMDLDAPCV
                     CLFAVDEPSQIYVLGPDENLLPSANHPEVNLYRNEDQWLLDRMERIDALSDGDCVRIS
                     GRMWEFVQGETLDATQEVSPPDGLLDAQLLQFDFRLSQNEEHASLDVRVGQQVISLGE
                     RIHHYTLATLARQRLRDARQGVDSPSQGWIALDNLARMLGVDPPYINIQMFRARQQIS
                     RALPPVRQPASLFERRRGEIRFGDHPFRIMKGVTEEGRFPYALQ"
     gene            complement(1162..2766)
                     /locus_tag="PKN55_11275"
     CDS             complement(1162..2766)
                     /locus_tag="PKN55_11275"
                     /inference="COORDINATES: protein motif:HMM:NF014661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOMM precursor leader peptide-binding protein"
                     /protein_id="HNX71868.1"
                     /translation="MAWLPLRVAGDTAWLGPWFGHEAGQPCWHCLDARLMRNQPVRAW
                     WRSRPAGQRAGLPESHQPDATALRLRLLAEALVRHGPNMPLVTVGQNGEMRAHPSPRR
                     PQCPACGDPGVLAARQAQPLRLAPARKLAGVAGGARTQTSQDTVTRLLAEVSPLTGVV
                     ADVRLLPGGGAMPVHHSQFFRPWRSDGAPRIQAQSCLGKGASEAQSCASALCEAVERY
                     AAAWQGDEAMRLARAAELPAPAITPDMLSFFSAEQRASARPGERAAQRAYDPQTPQAW
                     TPAWSLTENALRYLPLAACYADAPAPWADFAGWSSNGCASGNCREEAILQGLLEVIER
                     DAVAIWWYNMISRPQARCAAAAQARAAQALGPAWRCWLLDLTHDLGVPVLAAIAWREA
                     DGAWALGFGASLSAELAAERALTELVQLVAADKCLTADAVGPHAGFLWPAGERAPQDA
                     ARHDDIVDDILCCVAAWRAQGHEVIVHDYSRPDIALATVKVVTPGACHIWPERANPRL
                     FAAPVALGWLAAPLAPEALNFSDLYV"
     gene            complement(3253..4233)
                     /locus_tag="PKN55_11280"
     CDS             complement(3253..4233)
                     /locus_tag="PKN55_11280"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF015998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNX71869.1"
                     /translation="MLPHACGDGHHVLTLCLCKPDNFVDLRLKAVGSVQIGDGGRVMR
                     DEVVMARQAWAALADSSATLALTLAARAVAPTRLVDLNWHDPLAQRIAQACPLPLNEL
                     TQDDSLVRGIVLRSVLFDQCVLAAGLRAGSCVVTLGAGLCTRRSRLRAKLDEKVRWVN
                     VDLPPVAAVRELTISVEEGGDVWAGSVLDAQAWLTAVNLVEGAPHLLLLEGVLPYLSA
                     DEIRGLLDAIGAYFTRHHLFAWVVADFLHPAMAAPSAQGGLHLPVLSGFADAQALIAG
                     RAEWSIQSQRHPFAEFSAGHAKFAADFVLNHGLPPYTVACLTVGELVESI"
     gene            complement(4346..>4575)
                     /locus_tag="PKN55_11285"
     CDS             complement(4346..>4575)
                     /locus_tag="PKN55_11285"
                     /inference="COORDINATES: protein motif:HMM:TIGR03795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="BMA_0021/BMA_0022 family TOMM bacteriocin"
                     /protein_id="HNX71870.1"
                     /translation="SQWLGYNSPFNFKIKFVTNPGLHWDGKKWNLDDADGQPIKNRIV
                     LNYPVAPKEQALQPIALTSYNNTGPAYPFTC"
BASE COUNT          989 a         1525 c         1316 g          745 t
ORIGIN      
        1 tacaagcagt acggcaaggt cgggcaacga cgccaggagg gttttgttag ccgctattat
       61 tgcaaggcgt aaggaaagcg gccctcttcc gtcaccccct tcatgatgcg gaacggatgg
      121 tcgccaaagc ggatttcgcc gcgccggcgc tcaaacaggc tggcgggctg gcgcacaggc
      181 ggcaaggcgc gactgatctg ctggcgggcg cgaaacatct ggatattgat gtaaggcgga
      241 tccacgccca gcatgcgcgc cagattatcc agggcaatcc aaccctggct gggggaatcc
      301 acgccctggc gggcgtcgcg caaacgctga cgggccaagg tggccagcgt gtaatggtgg
      361 atgcgctcgc ccagcgaaat gacttgctgg cccacgcgca catccaggct ggcgtgttct
      421 tcgttttggc tcagccggaa atcaaactgc aagagctggg catccagcaa accgtctggc
      481 gggctgactt cttgcgtggc gtccagcgtt tcgccctgaa caaactccca catccggccg
      541 ctgatgcgca cgcaatcgcc atcagacaag gcgtcaatgc gctccatgcg gtcaagcaac
      601 cattggtctt cgttgcgata cagattgacc tctgggtgat tggcgctggg cagcaggttt
      661 tcatctggtc ccagcacata tatttggctg ggctcatcta ccgcaaacag gcacacgcat
      721 ggcgcgtcca aatccatgac agtccattgc gcctgctggc tggcggcaaa gcggatgaca
      781 tcgcccattt gcagcacgcg ccactgccct ggctgcagct tgtcgccact gacataggtg
      841 ccgttgcggc tctggtcgca gatttcccac agcgcgccgt tccagcgcac cacggcgtgc
      901 agcttggaaa catccgcctc ctgcaacacc gtgcgcgacg atgccgggtg gcggccaaat
      961 gtgtgcaccg ggcgcaacat gacgcggcgg cgcaaatgct tgttatgcaa aactgccatg
     1021 atgtgctcct gacttgacca agaaacaacg cccattccat gcttcgagcg ccaatacacc
     1081 ggcccgttac agaaaagcga tataaatcaa gtggagtcac acaaactcta ccgctgtccc
     1141 gcccgacaag tgcagacagg attatacgta caaatcagaa aaattcagtg cttctggcgc
     1201 cagcggcgcc gccagccagc ccagcgccac aggcgccgca aacaggcgag ggttggcgcg
     1261 ctcgggccag atgtggcacg cgcccggcgt caccaccttc acggtggcca gggcgatgtc
     1321 cgggcggctg tagtcatgca cgatgacctc atgcccttgc gcgcgccagg cggcgacaca
     1381 gcacagaatg tcatcgacaa tgtcgtcatg ccgggcggcg tcttgtggcg cacgctcgcc
     1441 ggctggccat aaaaacccgg cgtgcggccc caccgcgtcg gccgtcaggc atttgtcggc
     1501 ggcgaccagt tgcaccaact cagtcaaggc gcgctccgca gccagctcgg ccgacaggct
     1561 ggcgccaaac cccaaggccc aggcgccatc cgcctcgcgc caggcaatcg ccgccaacac
     1621 tggcacgccc agatcatggg tcaggtcgag caaccagcat cgccaggcag gccccagcgc
     1681 ttgcgccgcc cgcgcctgtg cggcggccgc gcaccgcgcc tgcggccggg aaatcatgtt
     1741 gtaccaccag atggcgacag cgtcgcgttc aatcacctcc aacaggcctt gcaaaatggc
     1801 ctcttcgcgg cagttgccgc tggcgcagcc attggagctc cagccggcaa agtcggccca
     1861 cggcgccggc gcgtcagcgt agcacgccgc caaaggcaga tagcgcagcg cgttttcggt
     1921 cagcgaccag gcgggcgtcc acgcctgcgg cgtctggggg tcataagcgc gctgggcggc
     1981 gcgttcgcca gggcgcgcgc tggcgcgttg ttcggcgctg aaaaagctca gcatgtccgg
     2041 agtgatggcg ggcgcgggca actccgccgc gcgcgccagc cgcatggcct cgtccccctg
     2101 ccaggcggcg gcgtagcgct ccaccgcctc acacaaggcg ctggcgcaag attgcgcctc
     2161 gctggcgcct ttgcccaggc agctttgcgc ctgaatgcgc ggcgcgccat cgctgcgcca
     2221 aggccgaaaa aactgactat ggtgcaccgg catggcgccg ccgcccggca gcaggcgcac
     2281 atccgccacc acgccggtca gcgggctgac ttcggccaat aagcgcgtca cggtgtcttg
     2341 tgaggtttgc gtgcgcgcgc cgcccgccac cccagccaat ttgcgcgctg gcgccaggcg
     2401 cagcggctgc gcctggcgcg ccgccagcac gccggggtcg ccgcatgcgg ggcattgcgg
     2461 ccggcgcggg ctggggtgag cgcgcatctc gccgttttgg cctacagtca cgagcggcat
     2521 attagggccg tgtctgacca atgcctcagc cagcaaacgc aaacgcaggg ctgtcgcatc
     2581 tggctgatgg ctctctggca aacctgcgcg ctggccagct ggtcgtgagc gccaccaggc
     2641 ccgaaccggc tgattacgca ttagccgagc atccaagcaa tgccagcatg gttgcccggc
     2701 ttcatgccca aaccagggcc caagccaagc cgtgtcgcca gccacccgca acggcagcca
     2761 agccatgcca gcggcgcgac actcagcgtc cagctgcgca aggcgtggat caaaataatc
     2821 atcacacaac actaaacgca ccgccgtcgg cagtggagcc agggccgcag cccaactcaa
     2881 cgccgtctct ggcgcaagcc gcgtgcttaa gcaagcgagc tccagcacgg gagaaatgcg
     2941 caaccacacc ggcgtcgcgc caaaatccgg gacggcccac cccaccggcg ccgtcgcagc
     3001 atcaaccacc cagccttgtt gcgccaactg cgccaatgcg tgcagcacgg taggcgcgtg
     3061 agccagccat gcagggtcgt gctcacaggc cttggctaag gactggttct gagcaatcag
     3121 cgccgccact tgccgcacag cgagcgcagt aaaccgccac atcccatcac catcaagcag
     3181 ggtgacgtcg tgctcggcaa cttccacctg ataacgtgga tgccagatgg gcgtgtggtt
     3241 aaaccaatcc attcagatag actccaccag ctcgcctacc gtcaaacacg cgaccgtgta
     3301 gggcggcaga ccatggttca ggacaaaatc ggcggcaaat ttggcgtgcc cggcagaaaa
     3361 ctctgcaaac gggtggcgtt gtgactgtat cgaccattct gctcgcccgg caatcagcgc
     3421 ttgtgcatca gcaaacccgc taagcactgg cagatgcagg ccaccctgtg cagaaggcgc
     3481 cgccatggcc gggtgtaaaa aatccgccac gacccaggca aataaatgat ggcgagtaaa
     3541 atatgccccg atagcgtcca gcaaacccct gatttcatca gccgacaaat atggcagcac
     3601 accttccagc agcagcaaat gcggcgcccc ttcaacaaga ttgaccgcag tcagccacgc
     3661 ctgcgcatcc agaacagaac cggcccaaac atctccacct tcttcaacac tgatggtcag
     3721 ttcgcgcacg gcagccacag gcggcagatc tacattaacc cagcgcacct tctcgtccag
     3781 cttggcgcgc aaacggctgc gtcgagtgca taagcccgcc cccaaagtca ccacgcaact
     3841 gcctgcgcgc aagccagccg ctaaaacaca ttggtcaaac aacacgctgc gcaacacaat
     3901 gccgcgcacc aaactgtcgt cttgtgtcag ctcattcaga ggcaacgggc acgcttgtgc
     3961 aatacgctga gccaaaggat catgccagtt caaatccacc aaacgcgtcg gcgccacggc
     4021 tcgagcagcg agcgtcaacg ccaacgtggc ggaactatca gccaatgcag cccaagcctg
     4081 ccgggccatc accacttcat cacgcattac acgtccacca tcaccaattt gcacagagcc
     4141 cactgccttc aacctgaggt ccacaaaatt atctggcttg cacaagcaca aagtgagcac
     4201 atgatgccca tcgccacagg catggggcag cacacaacag cgccaaaagg gtttgtgagc
     4261 cgcaagaatc gctcacaaaa cctttgagga agcaccaagc gccgctaaca aaactcagag
     4321 cagcaatcct ggcaggctgc cagaatcagc acgtgaaggg ataagccggg ccagtgttat
     4381 tataagaggt cagcgcaatc ggctgcagcg cttgttcttt gggtgccacc gggtagttca
     4441 agacaatgcg atttttgata ggctgcccgt ctgcgtcatc cagattccat tttttcccat
     4501 cccaatgcaa accaggattg gtcacgaatt tgatcttgaa attgaacggg ctgttatagc
     4561 ccagccactg gctta
//
LOCUS       DAOPYX010000243         4567 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_24307_length_4567_cov_9.403590, whole genome shotgun sequence.
ACCESSION   DAOPYX010000243 DAOPYX010000000
VERSION     DAOPYX010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 4567)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4567)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4567
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24307_length_4567_cov_9.403590"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..614
                     /locus_tag="PKN55_11290"
     CDS             <1..614
                     /locus_tag="PKN55_11290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011137240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HNX71871.1"
                     /translation="EHFVNALTTNLTYFFREEHHFPILAEHLRTLSGEVNIWCCAAST
                     GEEPYSLAMTALETASSGARLNVNIVATDLDTNVLRVGAEGIYGAEAINKLPAGMANR
                     YFDRVDDQRWRAKPALRQMIRFQQLNLVDTQWSVRGPFDAIFCRNVMIYFDRDTQLAV
                     LRRFAPLLKPDGLLFVGHSENFYHATDLFRLRGKTVYERVSKA"
     gene            760..2055
                     /locus_tag="PKN55_11295"
     CDS             760..2055
                     /locus_tag="PKN55_11295"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018747362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008718 - D-amino-acid dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0055130 - D-alanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HNX71872.1"
                     /translation="MKVVVLGAGVVGVSTAWFLAKAGHEVIVIDRQAQAGIETSYANG
                     GQISVSQSEPWANPGTPMRALKWMSKEDAPLLFRWRFDSRQWSWALKFLTECRPGRAK
                     QNIRQLLNLGLYSRRTLQDLRAETGIHYDEQTRGILAIYQTQQALDEAANTCLLMQTY
                     GMDRKVVTRDQIVEIEPALRHIAPKLAGGTYCPSDESGDARMFTQRLALLCEAEGVEF
                     RYHTRINALLTRGSHLSGVSITGSDGQYETLTADAFVMAMGSYSPLLARTIGINLPIY
                     PAKGYSATVPIEEPEVEVQNGEFVEGDNAPMVSITDEEHRLVFSRLGDRLRIAGTAEF
                     NGYSTELNAVRCTALINRAKQLFPSGADYSNAMFWTGLRPVTPSNVPMIGRTKEYNNL
                     FLNTGHGALGWTQGPGSGRALADIINGDRPEVDFRFMGL"
     gene            2140..3357
                     /gene="purT"
                     /locus_tag="PKN55_11300"
     CDS             2140..3357
                     /gene="purT"
                     /locus_tag="PKN55_11300"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012696104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HNX71873.1"
                     /translation="MSELFFGAPLSPNALKVMLLGSGELGKEVVIALQRLGVETIAVD
                     RYANAPAMQVAHRSHVIAMTDAAALRALVESERPHLIVPEIEAIATDELARIEADGLA
                     EVIPTARAAQLTMNREGIRRLAAETLGLPTSPYAFASSLAELQAAIDAGIGYPCVIKP
                     VMSSSGKGQSLVKTADALAAAWDYAASAGRVDAGRVIVEGFIDFDYEITQLTVRARNA
                     DGDIDTYFCEPIGHLQQHGDYVESWQPQAMSPAARQKARDYAAAVVNNLGGRGLFGVE
                     LFIRGDEVWFSEVSPRPHDTGLVTLASQTQSEFELHARAILGLPVDTAMRAPAAASAV
                     IYGGMDAQAIGFAGVAHALAVPGSDLRLFGKPESFIRRRMGVALASGEHETQTRERAK
                     LAASRVTPVQGKP"
     gene            3360..3785
                     /locus_tag="PKN55_11305"
     CDS             3360..3785
                     /locus_tag="PKN55_11305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018150017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II truncated hemoglobin"
                     /protein_id="HNX71874.1"
                     /translation="MTTATPTESNPLHTVTPYELLGGEAMLALLVDRFYNVMDHDPAV
                     AEIRRLHPADLEGSRQKLFMFLSGWLGGPNLYMEQFGHPRLRARHLPFPINQSASDQW
                     MYCMEKAFEGLAVDPTLRDQLMAALRNTANFMQNQQPHP"
     gene            3803..4261
                     /locus_tag="PKN55_11310"
     CDS             3803..4261
                     /locus_tag="PKN55_11310"
                     /inference="COORDINATES: protein motif:HMM:NF013933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HNX71875.1"
                     /translation="MQHWTALLGITAAIGIGAASPGPSFVMVARTAASQGRPHGLLAA
                     AGIGVGGLIFAILALLGLHSLLNLAPALYLALKLAGGLYLAWLGWRIWPVFRRKRKIA
                     TRFGVWCARIVTSNALCRSVVTHNTRMLRTRPRQKAGSASNTQSINPCSS"
     gene            4249..4404
                     /locus_tag="PKN55_11315"
     CDS             4249..4404
                     /locus_tag="PKN55_11315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX71876.1"
                     /translation="MLILIRQNGSEENGWRGAPFYWPIISAQENAQTTIFSHETQKNA
                     DVQESDE"
BASE COUNT          929 a         1547 c         1324 g          767 t
ORIGIN      
        1 tggagcactt tgtcaacgcg ctgaccacca acctgaccta tttctttcgc gaagagcatc
       61 actttcccat tctggccgag cacctgcgca cgctcagcgg tgaggtgaac atctggtgct
      121 gcgcagcctc caccggcgaa gaaccctatt cgctggccat gaccgcactg gaaaccgcca
      181 gcagcggcgc gcggttgaac gtcaatatcg tggcgaccga tctggacacc aatgtcctgc
      241 gcgtcggcgc cgagggtatt tatggcgccg aagcgatcaa caagctgcca gccggtatgg
      301 ccaatcgcta ttttgatcgg gtagatgatc aacgctggcg cgccaagccg gccttgcgcc
      361 agatgatccg ctttcaacaa ctgaatctgg tcgatacgca atggtcggtg cgcgggccgt
      421 ttgacgcgat tttctgccgc aatgtgatga tttatttcga ccgcgacacc caactggcgg
      481 tattgcgccg ctttgcgcca ctgcttaaac cggatggcct gttgtttgtg ggtcattcgg
      541 agaattttta tcacgcgacc gatcttttcc gcctgcgcgg caagaccgtg tacgagcggg
      601 tcagcaaggc gtaatcccgc acagcgggct atggcgcacc tcaacggcgt gtcataatct
      661 gacgcaagaa caactcccgg cgcatgcgca gccttgggcg cctgccggtt atggaccgca
      721 atcacgccag ccccgctggc acaagaatga gaaaacccta tgaaagtcgt ggtgttgggc
      781 gccggcgttg tcggagtttc cactgcctgg ttcttggcca aggccggtca tgaagtcatc
      841 gtgattgatc gccaggcgca agccggcatc gaaaccagct atgccaatgg cggccagatt
      901 tctgtcagcc agtccgagcc gtgggccaac cccggcacgc cgatgcgcgc gctgaaatgg
      961 atgtccaaag aagacgcccc gctgctgttt cgctggcgct ttgatagccg gcaatggtcg
     1021 tgggcgctga aatttctcac tgaatgccgt ccgggccgcg ccaagcagaa tatccgccag
     1081 ttgctcaatc tggggctgta cagccggcgc acgctgcaag acctgcgcgc cgaaaccggc
     1141 atccactacg acgaacaaac ccgtggcatc ctggccatct accaaaccca gcaagcgctg
     1201 gacgaagcgg ccaacacctg tttgctgatg caaacctacg gcatggaccg caaggtggtt
     1261 acccgcgacc agattgtgga aatcgaaccg gccctgcgcc atatcgcccc caagctggcc
     1321 ggcggcacct attgccccag cgacgaatcc ggcgacgcac gcatgttcac ccagcgcctg
     1381 gccctgctgt gcgaagccga aggcgtggaa ttccgctacc acacccgcat caatgccctg
     1441 ctcacccgtg gcagccacct gagcggcgtg tccatcaccg gctcggatgg ccagtacgaa
     1501 acgctgaccg ccgacgcctt tgtgatggcc atgggcagtt attcgccgct gctggcgcgc
     1561 accatcggca tcaatctgcc gatttacccg gccaagggct attccgccac cgtgccgatt
     1621 gaagaacccg aagtcgaagt gcaaaacggc gaatttgtcg aaggcgacaa cgcgccgatg
     1681 gtcagcatca ccgacgaaga acaccggctg gtgttctccc ggctgggcga ccgcctgcgc
     1741 attgccggca ccgctgagtt caatggctat tccaccgagc tgaacgccgt gcgctgcacc
     1801 gcgctgatca accgcgccaa gcagctgttc cccagcgggg ccgactacag caacgccatg
     1861 ttctggaccg gcctgcgccc ggtgacgccg tccaatgtgc cgatgattgg ccgcaccaag
     1921 gaatacaata atctgttcct gaacaccggc catggcgcgc tgggctggac acaaggcccc
     1981 ggctcgggcc gcgcgctggc cgacatcatc aacggcgacc gcccggaagt ggatttccgc
     2041 ttcatgggcc tgtaaacccg cttcaggccg cgccacacgc cccgccccgc ccgcgcctgg
     2101 cggggtgaag ttattgcggc ccccacgagg atacaagtca tgtctgaact gttttttggc
     2161 gccccgctct cgcccaatgc cctgaaagtc atgctgctgg gcagcggcga gctgggcaaa
     2221 gaagtcgtca tcgccctgca acgcctgggc gtggaaacca tcgccgtgga ccgctacgcc
     2281 aacgccccgg ccatgcaagt ggcgcaccgc agccatgtga tcgccatgac cgacgccgcc
     2341 gcgctgcgcg cgctggtgga aagcgagcgc ccgcacctga tcgtgccgga aatcgaagcc
     2401 atcgccaccg acgagctggc gcgcatcgaa gccgacggcc tggccgaagt catccccacc
     2461 gcccgcgccg cgcaactgac catgaaccgc gaaggcatcc gccgcctggc cgccgaaacc
     2521 ctgggcctgc ccacctcgcc ctacgccttt gccagcagcc tggccgagct gcaagccgcc
     2581 atcgacgccg gcatcggcta cccctgcgtg atcaagccgg tgatgtcgtc aagcggcaag
     2641 gggcaaagcc tggtgaaaac cgccgacgcg ctggccgccg cctgggatta cgccgccagc
     2701 gccgggcggg tggacgccgg ccgggtgatc gtggaaggct ttatcgactt tgactacgaa
     2761 atcacccagt tgaccgtgcg cgcccgcaac gccgatggcg acatcgacac ctatttttgc
     2821 gagcccatcg gccacctgca acaacacggc gactacgtgg aaagctggca gccgcaggcc
     2881 atgagcccgg ccgcccggca aaaagcgcgc gactacgccg ccgccgtggt caacaacctg
     2941 ggcggacgcg gcctgtttgg cgtcgagctg ttcatccgtg gcgatgaagt gtggttctcc
     3001 gaagtcagcc cgcgcccgca cgacaccggc ctggtcaccc tggccagcca gacgcaaagc
     3061 gaatttgagc tgcacgcccg cgccatcctc ggcctgccgg tggacaccgc catgcgcgcg
     3121 ccggccgccg ccagcgcagt gatttacggc ggcatggacg cccaggccat cggctttgcc
     3181 ggcgtggccc acgccctggc cgtgcccggc agcgacctgc gcctgtttgg caaaccagaa
     3241 agctttatcc gccgccgcat gggcgtggcg ctggccagcg gcgaacacga aacccaaacc
     3301 cgcgaacgcg ccaaactggc cgccagccgc gtcacccccg tgcaaggaaa accctgatca
     3361 tgacgaccgc cacccccacc gaaagcaacc cgctgcacac cgtcaccccg tatgaactgc
     3421 tgggcggcga agccatgctc gccctgctgg tggaccgctt ttacaatgtg atggaccacg
     3481 acccggcggt ggccgaaatc cgccgcctgc acccggccga cctggaaggc tcgcgacaaa
     3541 aactgttcat gtttctgtcc ggctggctgg gcggccccaa tctgtatatg gagcaatttg
     3601 gccacccacg cctgcgcgcc cgccatttgc cgttccccat caatcaatcg gccagcgacc
     3661 aatggatgta ctgcatggaa aaagcctttg aaggcctggc agtcgatccc accctgcgcg
     3721 accaactgat ggctgcgttg cgcaataccg ccaatttcat gcaaaaccag cagccccacc
     3781 cctgaacgcc acaggagccc ccatgcaaca ctggaccgcc ctactgggca tcaccgccgc
     3841 cattggcatc ggcgccgcca gccccggccc cagttttgtc atggtggcgc gcaccgccgc
     3901 cagccagggc cgcccgcatg gcctgctggc cgccgccggc attggcgtgg gcgggctgat
     3961 attcgccatc ctggcgttat tgggcctgca cagcctgctc aacctggccc ccgccctata
     4021 cctggcgctg aaactggccg gcgggctgta tctggcctgg ctgggctggc gcatctggcc
     4081 ggtctttcga agaaagcgga aaatcgcaac aaggtttggt gtctggtgcg caagaatcgt
     4141 aacctcaaac gcactgtgtc ggtcggtagt cacgcacaat acgcggatgc tccggactcg
     4201 accaagacag aaggccggct ccgccagcaa tactcaatcg atcaacccat gctcatcctg
     4261 atccgccaaa atggatcaga agaaaacgga tggcgaggcg ccccgtttta ctggccaatc
     4321 atctctgctc aagaaaatgc tcagacgacc attttctcgc atgagacaca gaagaatgcc
     4381 gatgtacaag agagtgacga ataagtaaga gtggctaaca aaacccagcg cagcgatcct
     4441 ggctaggcgc acgaccgcag acagtacaag cagtacggca aggtcgggca acgacgccag
     4501 gagggttttg tgagcggctc taagtcaacc aattcgagtg agactcagcg atgcaccata
     4561 agacaaa
//
LOCUS       DAOPYX010000244         4560 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_24373_length_4560_cov_9.782686, whole genome shotgun sequence.
ACCESSION   DAOPYX010000244 DAOPYX010000000
VERSION     DAOPYX010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 4560)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4560)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4560
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24373_length_4560_cov_9.782686"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1178)
                     /locus_tag="PKN55_11320"
     CDS             complement(<1..1178)
                     /locus_tag="PKN55_11320"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014085881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HNX71877.1"
                     /translation="MTQLSEWLKQHQITEVECVTPDFTGIARGKIVPREKFSADEGMR
                     LPEAVLVQTVTGEFADNVTPATDPDMILLPDPNSIRLVPWAKDPVAQVIHDCYNSDGT
                     PVDLSPRYVLRRVLKLYEDMGWEPVVAPEMEFYIVDVNRDPDLPLQPPLGRTGRPETG
                     RKAYSIDAVNEYDDMFEDIYDYCDAQNLAIDTLIHEVGACQMEINFLHGDALDLADQV
                     FLFKRTVREAAIRHNMYATFMAKPMENEPGSAMHIHMSLKDRQTGKNVFSNPDGSPSE
                     AFLHAIGGMQKYLPQVIALFAPFVNSYRRLVRFNAAPINVQWGYDNRTCGIRVPYSKP
                     EARRIENRLPGVDCNPYIALAATLACAYLGLTEKCQPSEPQTGDAYGLPFAFARGLDE
                     A"
     gene            complement(1297..2049)
                     /locus_tag="PKN55_11325"
     CDS             complement(1297..2049)
                     /locus_tag="PKN55_11325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014087390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HNX71878.1"
                     /translation="MTLPVIGLPCCRWWLKDSQFFHLVGEKYLRAATWAGGAPILLPA
                     LGEEAPLAQWLDMVDGLLFTGSPSNIEPHHYGEARREGDFNDPERDATTLPLVRAALD
                     AGVPLLGICRGLQEINVALGGSLHGQVHALPGMRDHREQGDTLDAQYGLAHPLTLAEG
                     GVLHQALGATHAEVNSLHGQGIRTLGAGLRAEAWALDGLIEAFSVAEAKSFAVAVQWH
                     PEWRIDAHPLSRALFTAFGDACRQRRAARLAA"
     gene            2356..2907
                     /locus_tag="PKN55_11330"
     CDS             2356..2907
                     /locus_tag="PKN55_11330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008953488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNX71879.1"
                     /translation="MDIGVRLKLVRERFGLSQRELAKRAGVTNASISLIEQNRVSPSV
                     SSLTKLLESIPITLSEFFAFDEPPRVNPHIFRPDNQPAFLSSDGLRFLVIGASAPNRQ
                     MRMLRELYAPGAHTGDEPIVHKEGEECGFVVRGTIELTIDGQANILSAGDGYYFPSTL
                     PHRFRNIGPEEAEIISANTPASF"
     gene            2986..4473
                     /locus_tag="PKN55_11335"
     CDS             2986..4473
                     /locus_tag="PKN55_11335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011134777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HNX71880.1"
                     /translation="MTQPTLADWQARAAALTLEGRAFINGQYVNAASGRVFATRNPAT
                     RQVLCQVAECGADEVNAAVSAARAAFTAGVWAEMAPMLRKRTLLRFADLLRANRDELA
                     LLETLDTGKPISDARAVDVAGAAYCVQWFAETVDKIGGEVAPLASNLLGTVTREPLGV
                     VAAVVPWNFPLLMASWKFAPALAAGNSVILKPSEKSPLTAIRIAALAHEAGIPAGVFQ
                     VLPGDGEVGKLLSLHMDVDCLAFTGSTAVGKRIMGYAAESNLKRVWLELGGKSPNIVL
                     ADCPDITAAARAAAGGIYFNQGEMCSAGSRVLVHNSIYNEFVAAFKEAARAYAPGNPL
                     DPATRMGAIVDEVQYERVLGYIQQGRNEGRCLMGGNGVMTEQGYFITPTLFEASPHSR
                     IAREEIFGPVCTLIGFDDDDEAIAIANDSEYGLAAALWTRDVSRAHRLSRRLRAGTVW
                     VNCYDEGGDMSLPFGGYKQSGNGRDKSLHALEKYTELKTTLLKLG"
BASE COUNT          819 a         1435 c         1472 g          834 t
ORIGIN      
        1 gcttcgtcca ggccacgggc aaaggcaaac ggcaggccgt aggcgtcgcc ggtttgcggc
       61 tcgctgggct ggcatttttc cgtcaggccc aggtaggcgc aggccagggt ggcggccagg
      121 gcgatatacg ggttgcagtc cacgcctggc agacggtttt caatgcggcg ggcttcgggc
      181 ttggagtagg gcacacggat gccgcaggtg cggttgtcgt agccccattg cacattgatc
      241 ggcgcggcgt taaagcgcac caggcggcgg tagctgttca caaacggggc gaacagggca
      301 atcacctgcg gcaggtattt ctgcatgccg ccgatggcgt gcaaaaacgc ctcgctgggg
      361 ctgccgtccg ggttggaaaa cacattcttg ccggtttgcc ggtctttcag gctcatgtgg
      421 atgtgcatgg ccgagcccgg ctcgttttcc atcggcttgg ccatgaaggt ggcgtacata
      481 ttgtggcgga tggccgcctc gcgcaccgtg cgcttgaaca agaacacctg gtcggccagg
      541 tccagcgcgt cgccatgcag gaagttgatt tccatctggc aggcgcccac ttcgtgaatc
      601 agcgtgtcga tggccagatt ttgtgcgtcg cagtagtcgt agatgtcctc gaacatatcg
      661 tcgtactcgt tcaccgcgtc gatcgagtag gccttgcgtc cggtttccgg gcggccggtg
      721 cggcccagcg gcggttgcag cggcagatcg ggatcgcggt tgacgtcaac gatgtagaac
      781 tccatctccg gcgccaccac cggctcccag cccatgtcct cgtacagctt gagcacgcgg
      841 cgcagcacat agcgcggcga cagatccacc ggcgtgccgt cgctgttgta gcaatcgtga
      901 atcacctgcg ccaccgggtc tttcgcccag ggcaccaggc ggatgctgtt ggggtcgggc
      961 agcagaatca tgtccgggtc ggtggccggg gtgacgttgt cggcaaattc gccggtcacg
     1021 gtttgcacca gcaccgcctc gggcaggcgc atgccttcat cggcgctgaa tttttcgcgc
     1081 ggcacgatct tgccgcgggc aatgccggtg aaatccggcg tcacgcattc cacttcggtg
     1141 atttgatgtt gtttcagcca ttcgctcagt tgggtcatgg tgcgtcctcg tgggtgggtt
     1201 ttctgtcaga aaccctcaca gaatgcgact gctgtattgc gccgcattgt gtgagcgctt
     1261 ctccgccctg atcctggtgg agtcagggcg cggggtttac gccgccaagc gggcggcgcg
     1321 acgctggcga caggcgtcgc caaaggcggt gaacagtgcg cgcgacagcg gatgcgcgtc
     1381 gatgcgccat tccggatgcc attgcaccgc cacagcaaaa ctcttggcct cggccacgct
     1441 aaaagcctca atcagcccat ccagcgccca ggcttcggcg cgcaggccgg cgcccagggt
     1501 gcggatgccc tggccatgca gggaattcac ttcggcgtgg gtggcgccca gcgcctggtg
     1561 caacacaccg ccttcggcca gggtcagcgg gtgggccagg ccatattgcg cgtccagcgt
     1621 gtcgccttgc tcgcggtggt cgcgcatgcc gggcagggca tgcacttggc cgtgcaggct
     1681 gccgccgaga gcgacgttga tttcttgcag gccgcgacaa atgcccagca gcggcacgcc
     1741 ggcgtccagc gcggcgcgca ccagcggcag ggtggtggcg tcgcgctcgg ggtcgttgaa
     1801 atcgccttca cggcgggctt cgccgtagtg gtgcggctcg atattggatg ggctgccggt
     1861 gaacagcagg ccgtccacca tgtccagcca ctgcgccagc ggcgcctctt cgcccaaggc
     1921 cggcaagagg atgggcgcgc cgccggccca ggtggcggcg cgcaggtatt tctcgcccac
     1981 caggtggaaa aactggctgt ctttcagcca ccagcggcag cagggcaaac cgattacagg
     2041 aagcgtcatc gcaggtctct cacaatcaca tccgcgccat catgcatatg gtgtgttgca
     2101 tggcatggct taagttcgtg tatccagtct agcaacgtta aaaataattg acaacaggcg
     2161 ttaaaaatat tttgatatgg gctgtggcaa ccagagacac tcgctcgaaa atagtgccgc
     2221 actgcaacat aatggtgcgg aaatacaggg aatccgggga aatcgggtat actcaagcga
     2281 cggcggcagc cccgctgatt tcgtcaacta ttctttacag cgagcgtcaa ttattatgaa
     2341 cacacaaggg ttgccatgga cataggtgta cgcctcaaac tggtgcgtga gcgctttggc
     2401 ctgtcgcagc gcgagctggc caagcgcgct ggcgtgacca atgcctcgat ctctttgatc
     2461 gagcaaaatc gggtcagccc gtcggtgagc tcgctcacca aactgctgga aagcatcccg
     2521 atcaccttgt ccgagttttt tgcttttgat gaaccgccca gggtgaatcc gcacattttc
     2581 cgtccggaca atcagccggc gtttctgagc agcgatggct tgcgctttct ggtgattggc
     2641 gcttcagcgc ccaaccggca aatgcgcatg ctgcgcgagt tgtacgcccc cggcgcgcac
     2701 actggcgacg agcctatcgt gcacaaagag ggcgaagaat gcgggtttgt ggtgcgcggc
     2761 accattgaac tcaccattga tggccaggcc aatatcctga gcgcggggga cgggtattac
     2821 tttcccagca ccctgccgca ccgctttcgc aatattggcc cggaagaagc ggaaattatc
     2881 agcgccaaca ccccggccag cttctagctg gctgcgccgc ccggcagacc gggctggcgc
     2941 gctggccggc agccggggga tggccgccca cacaggagat tcgccatgac tcaaccgact
     3001 cttgccgact ggcaagcgcg cgccgccgcc ctgaccttgg aaggacgcgc cttcatcaac
     3061 ggccagtacg tcaacgccgc cagcggtcgg gtgttcgcca cccgcaaccc ggccacccgc
     3121 caggtgctgt gccaggtggc cgaatgcggc gccgatgagg tcaacgccgc cgtcagcgcc
     3181 gcccgcgccg cctttactgc tggcgtctgg gccgagatgg cgccgatgct gcgcaagcgc
     3241 accctgctgc gctttgctga tctattgcgc gccaaccgcg acgaactggc cctgctggaa
     3301 accctggaca ccggcaagcc catcagcgac gcccgcgcgg tggacgtggc cggcgcggcg
     3361 tattgcgtgc agtggtttgc cgagacagta gacaaaatcg gcggcgaagt ggccccgctg
     3421 gccagcaatc tgctgggcac cgtcacccgc gagccgctgg gcgtggtggc cgccgtggtg
     3481 ccgtggaact ttccgctgct gatggccagc tggaaattcg ccccggcgct ggccgccggc
     3541 aactcggtga tcctgaaacc ttccgaaaaa tccccgctca ccgccatccg catcgccgcg
     3601 ctggcgcacg aggccggcat cccggccggg gtgttccagg tgctgccggg cgatggcgaa
     3661 gtgggcaagc tgttgagcct gcacatggac gtggactgcc tggcctttac cggctcgact
     3721 gccgtgggta agcgcatcat gggctacgcg gcagaatcca acctcaagcg cgtctggctg
     3781 gagctgggcg gcaaatcgcc caatatcgtg ctggccgact gcccggacat caccgccgcc
     3841 gcccgcgccg ccgccggcgg gatttatttc aatcagggcg aaatgtgctc ggccggctcg
     3901 cgcgtgctgg tgcacaacag catttacaac gaatttgtcg ccgccttcaa agaagccgcc
     3961 cgcgcctacg cgccgggcaa cccgctggac ccggccaccc gcatgggcgc gattgtggat
     4021 gaagtgcagt acgagcgcgt gctgggctat atccagcaag gccgcaacga aggccgctgc
     4081 ctgatgggcg gcaacggcgt gatgaccgag cagggttatt tcatcacccc gacgctgttc
     4141 gaggccagcc cgcacagccg cattgcccgc gaagaaatct ttggcccggt gtgcacgctg
     4201 atcggctttg acgacgacga cgaagccatc gccatcgcca acgacagcga atacggcctg
     4261 gccgccgcgc tgtggacccg cgacgtcagc cgcgcccacc gcctgtcgcg ccgcctgcgc
     4321 gccggcacgg tgtgggtgaa ctgctacgac gaaggcggcg acatgagcct gccgtttggc
     4381 ggctacaaac aatccggcaa cggccgcgac aaatcgctgc acgcgctgga aaagtacacc
     4441 gagctgaaaa ccacgctgct gaaactgggc tgagagcgac tctacaacct accgcagcgc
     4501 agcagatcaa gttaggggat tgcccgtcac gcgccaggga ggccgtgacg ggtttttttg
//
LOCUS       DAOPYX010000245         4547 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_24467_length_4547_cov_9.764025, whole genome shotgun sequence.
ACCESSION   DAOPYX010000245 DAOPYX010000000
VERSION     DAOPYX010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 4547)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4547)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4547
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24467_length_4547_cov_9.764025"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(226..867)
                     /locus_tag="PKN55_11340"
     CDS             complement(226..867)
                     /locus_tag="PKN55_11340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014086721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNX71881.1"
                     /translation="MNDAVSTLPVRLMLVDDHPLVRDGLRARLGAVAHLEVIGEASNG
                     HEALLRAAELCPDVMLVDIGMKEMNGLELTERLSQAHPAIRVLILSMYDTPEYYSRAI
                     AAGAKGYVLKDAGSHEIIAAIDAVAAGGSYYSTEVARTLFAQRHAQPGHGLSEREREV
                     LILLAQGLSNKAIALQLDISVRTAETHRLSVRRKLGIDSAAGLAKFAIEHGWL"
     gene            complement(864..2228)
                     /locus_tag="PKN55_11345"
     CDS             complement(864..2228)
                     /locus_tag="PKN55_11345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008953784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HNX71882.1"
                     /translation="MELKHKILALTVLPLLLAIVAVGALVQYRAERLAEQQAALLEES
                     LLASKRAELKHYVELALSSIEPLYANPRLPREQAQTQAKAILQGLSFGDDGYFFAYDT
                     GGRNLVHPRQPELVGRNLWDLTDPQGRHVIRALLAAARDGDGYQRYGWEKPSTHQMTE
                     KLGYVVLLPRWGWMLGTGIYLDDVERATAQVRERARGNARSTLLALGAVALVAVLLVF
                     AGGLALNVSEHRLADSKLKAMAQRIVQSQEDERARVSRELHDGISQLLVSVKFQFELA
                     QHQLDHGDERASDSMRQGLGRLGEAIGEVRRISHDLHPSLLDTLGLAAALDQLSREFA
                     QRTGIAVQQHTDHEMTALNRQPAKTVALFRIAQEGLCNIERHAHASLVDIQLCRHGNR
                     VELTLIDNGRGCPADALYASTGIGLRNIRERVEHLAGDFSLQSIPGRTVLRVSLPLER
                     EALL"
     gene            2515..>4547
                     /locus_tag="PKN55_11350"
     CDS             2515..>4547
                     /locus_tag="PKN55_11350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008953783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation CstA family protein"
                     /protein_id="HNX71883.1"
                     /translation="MNQLSRHALFGLVALVGAFALGTVALHRGESINALWIVVAAVSV
                     YLIAYRYYGLFIARTVMKLDANRATPAIVHNDGLDYVPTNKNVLFGHHFAAIAGAGPL
                     VGPVLAAQLGYLPGMLWILAGVVFAGAVQDFMVLFISTRRDGRSLGDLIKSEMGAVPG
                     VIALFGTFMIMVIILAVLALIVVKALAESPWGAFTVAATIPIALFMGVYSRYIRPGRI
                     GEISLIGFVLLMLAIWGGGQIAADPYWGAAFTFTGVQLTWMLIGYGFVASVLPVWLLL
                     APRDYLSTFLKIGTIVGLAIGILVVAPHLRMPALTQFVDGSGPVWAGNLFPFLFITIA
                     CGAVSGFHALISSGTTPKLLENESHMPYIGYGGMLMESFVAIMALIAASVIDPGVYFV
                     MNSPAAVVGKTAESAAAAVSNFGFLITPEMITHTAQTLGEKTIISRAGGAPTLAVGMA
                     EILSSVFGGDGMKAFWYHFAILFEALFILTAVDAGTRAGRFMLQDLLGTFAPSLRKTD
                     SWAASLTATGLCVAAWGYFLYQGVVDPLGGINTLWPLFGIANQMLAGIALTLATVVLF
                     KLKRERYAWVTIVPTLWLLVCTLTAGWQKVFADNPKIGFLAHAHKYQAALDKGELLAP
                     AKSLAQMQQVVFNDYVDATLAFGFILLVVSIVLFGVRACWKAWQSQQPTARETP"
BASE COUNT          717 a         1471 c         1459 g          900 t
ORIGIN      
        1 gcgtgtacaa gctggcgctg gccaagggct tgctcacccg cgaggcgctg gaagaaatcc
       61 tcaaaccaga acggctgacc cgcccgcgca agtttgtgcc gctgcgctga cacacacact
      121 gatgacgcac tgccaaccaa accggcgcgc agacacggga aaatcttgtg cgcagcgcac
      181 cggacggcgc ctgctgcgcc aggatgcggt gagttgcatt cgccgctaca gccagccgtg
      241 ctcaatggcg aatttggcca ggccggcggc cgaatcaatg cccagcttgc ggcgcacgct
      301 taaccgatgg gtttcggcag tgcgcacgct gatgtccagt tgcagggcga tggccttgtt
      361 ggacagcccc tgcgccagca ggatcagcac ttcgcgttcg cgctcggaca agccatggcc
      421 gggctgggcg tggcgctggg caaacagcgt gcgcgccacc tcggtgctgt aatagctgcc
      481 gccagcggcc acggcgtcga tggcggcgat gatttcgtgc gagccggcgt ctttcagcac
      541 atagcccttg gccccggcgg caatcgcccg gctgtaatac tccggggtgt cgtacatgct
      601 gagaatcagc acgcggatgg ccgggtgcgc ctggctgagt cgctcggtca gctccaggcc
      661 gttcatttct ttcatgccga tgtccaccag catcacgtcc gggcacagct cggcggcgcg
      721 cagcagcgct tcgtggccat tgctggcctc gccaatcacc tccagatgcg ccactgcgcc
      781 cagccgggcg cgcaggccgt cgcgcaccag cggatggtcg tccaccaaca tcaggcgcac
      841 cggcagggtg ctgaccgcgt cattcataac aaagcctcgc gttccagggg caggctgaca
      901 cgcagcacgg tgcgccctgg gatggattgc agagaaaaat cgcccgccag atgctccacg
      961 cgctcgcgga tattgcgcag gccaatgccg gtgctggcgt atagcgcatc cgccgggcag
     1021 ccgcggccgt tgtcgatcag cgtcagctcc acccggttgc cgtggcggca gagctggata
     1081 tccaccaggc tggcgtgggc gtgacgctcg atattgcaca gcccttcctg ggcaatgcgg
     1141 aacagcgcca cggttttggc cggctggcga ttcagcgcgg tcatctcgtg gtcggtgtgt
     1201 tgctgcacgg cgatgccggt gcgctgggcg aattcgcggc tgagttggtc cagcgcggcg
     1261 gccaacccca gggtgtccag caaagagggg tgcaggtcgt gcgaaatccg tcgcacctcg
     1321 ccaatcgcct cacccagccg gcccaggccc tggcgcatgc tgtcgctggc gcgctcgtcg
     1381 ccgtggtcca gctggtgctg ggccaattca aactgaaact tcaccgacac caataactgc
     1441 gaaatgccgt cgtgcagctc gcgcgacacc cgcgcgcgct cgtcttcctg cgattgcaca
     1501 atgcgctggg ccatggcttt gagcttgctg tcggccagcc ggtgttcgct gacattcagc
     1561 gccagcccgc cggcaaacac cagcagcacc gccaccagcg ccaccgcgcc cagcgccagc
     1621 aaggtgctgc gggcattgcc gcgcgcgcgt tcgcgcacct gggcggtggc gcgctcgaca
     1681 tcgtccagat aaataccggt gcccaacatc cagccccagc gcggcagcag caccacatag
     1741 ccgagttttt ccgtcatctg gtgggtggag ggtttttccc agccgtagcg ctggtagccg
     1801 tcgccgtcgc gggcggcggc cagcagggcg cggatcacat ggcggccttg cgggtcggtc
     1861 aaatcccaca gattgcgccc gaccagctcg ggctggcgtg gatgcaccag gttgcgtccg
     1921 ccagtgtcgt aggcaaagaa atagccgtca tcgccaaagc tcagcccctg caagatcgcc
     1981 ttggcctgtg tctgcgcctg ttcgcgcggc aggcgtgggt tggcgtacag cggctcgatc
     2041 gagcttaagg ccagctcgac atagtgcttg agctcggcgc gtttgctggc cagcaggctt
     2101 tcttccagca gggcggcctg ttgttcggcc aaccgctcgg cgcggtattg caccagcgcg
     2161 cccaccgcca caatcgccag cagcagcggc agcacggtaa gcgccagaat tttgtgtttg
     2221 agttccatgg ctttgctgca ccgcactacg tagtactgcg tagcggcggc cggtagtcgt
     2281 gcggatgtgg acagagacgc aaaaagccat agtagagccc aagcccggcc gctggcgcaa
     2341 gcccgcgccg tctgcgccat ggccacgccc ttgccggcgg gcgcagatcc gctgccggcg
     2401 caagccccct gccctcgtcc ccctcggcga gcggctggga gcttgctcac gcttgtctga
     2461 cgtcggcgcg ccaacgcagc gcgcccgcac atctaaatca tctggagaac tgtcatgaat
     2521 caattgtccc gccacgccct gttcgggctg gtggcgctgg tcggcgcatt cgcgctgggc
     2581 acggtggcgc tgcatcgcgg cgaatccatc aatgccctgt ggattgtggt ggccgccgtc
     2641 tcggtttatc tgattgccta ccgctactat ggcctgttta tcgcccgcac ggtgatgaag
     2701 ctggacgcca atcgcgccac cccggcgatt gtgcacaatg atggcctgga ctacgtgccc
     2761 accaataaaa acgtgctgtt cggccaccac tttgccgcca ttgccggcgc cggcccgctg
     2821 gtgggcccgg tgctggccgc ccagctgggc tatttgcccg gcatgctgtg gattctggct
     2881 ggcgtggtat ttgccggcgc agtgcaggat ttcatggtgc tgtttatctc cacccgccgc
     2941 gatggccgct cgctgggcga tttgatcaaa tcggaaatgg gcgccgtgcc gggggtgatt
     3001 gccctgtttg gcactttcat gatcatggtc atcatcctgg cggtgctggc gctgattgtg
     3061 gtcaaggcgc tggcggaaag cccgtggggc gcgttcaccg tggcagccac catccccatt
     3121 gctttgttca tgggcgtgta cagccgctat atccggccgg gccgcattgg cgaaatctcg
     3181 ctgattggct ttgtgctgct gatgctggcc atctggggcg gcggtcagat tgccgctgac
     3241 ccgtactggg gcgcggcgtt tacctttact ggcgtgcagc tgacctggat gctgattggc
     3301 tatggctttg tcgcctcggt gctgccggtg tggctgctgc tggccccgcg tgattatctg
     3361 tccaccttcc tgaaaatcgg caccatcgtt ggcctggcca tcggtattct ggtggtggcc
     3421 ccgcatctgc gcatgccggc gctcacccag tttgtcgatg gttctggccc ggtgtgggcg
     3481 ggcaatctgt tccccttcct gttcatcacc atcgcctgcg gcgcggtgtc gggttttcat
     3541 gcgctgattt cctcgggcac cacgcccaag ctgctggaaa acgagtcgca catgccctac
     3601 atcggctacg gtggcatgct gatggaaagc tttgtcgcca tcatggcgct gatcgccgcc
     3661 tcggtgatcg accctggcgt gtattttgtg atgaacagcc cggcggccgt ggtcggcaaa
     3721 accgccgaat ccgccgccgc cgcggtgtcc aacttcggct ttttgatcac cccggaaatg
     3781 atcacccaca ccgcgcagac cctgggtgaa aaaaccatca tctcgcgcgc tggcggcgcg
     3841 ccgactttgg cggtgggcat ggcggaaatc ctgtccagcg tgtttggcgg cgacggcatg
     3901 aaggccttct ggtatcactt tgccatcctg tttgaggcgc tgttcatcct caccgccgtg
     3961 gacgccggca cccgcgccgg gcgatttatg ttgcaggatt tgctcggcac ctttgccccc
     4021 agcctgcgca agactgattc gtgggcagcc agcctgaccg ccactggcct gtgcgtggcc
     4081 gcctggggtt actttttgta tcagggcgtg gtcgatccgc tgggcggcat caacaccctg
     4141 tggccgctgt ttggcatcgc caaccagatg ctggccggca ttgccctgac cctggccacc
     4201 gtggtgctgt tcaagctcaa gcgcgaacgc tatgcctggg tgaccattgt gccgaccctc
     4261 tggctgctgg tgtgcacgct gaccgccggc tggcagaagg tgtttgcaga caatcccaag
     4321 atcggttttc tggcgcacgc ccataaatac caggcggcgc tggacaaggg cgagctgctg
     4381 gccccggcca agagcctggc gcagatgcag caggtggtgt tcaacgacta tgtggatgcg
     4441 acgctggcct ttggtttcat cctgctggtg gtaagcatcg tgctgtttgg cgtgcgcgcc
     4501 tgctggaaag cctggcagag ccagcagccc accgcgcggg aaacgcc
//
LOCUS       DAOPYX010000246         4542 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_24516_length_4542_cov_6.353466, whole genome shotgun sequence.
ACCESSION   DAOPYX010000246 DAOPYX010000000
VERSION     DAOPYX010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 4542)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4542)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4542
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24516_length_4542_cov_6.353466"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..311
                     /locus_tag="PKN55_11355"
     CDS             <1..311
                     /locus_tag="PKN55_11355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX71884.1"
                     /translation="EYQAIHHAHPDEAAALLSQAEALLLPAEASQPGLAAYNLACIAA
                     LRQRPADAIHWLRSCQTHGTLPSEEHFRTDSNLDPIRDTPEFTAWWAEVFGRGDAAMG
                     "
     gene            complement(301..3696)
                     /locus_tag="PKN55_11360"
     CDS             complement(301..3696)
                     /locus_tag="PKN55_11360"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNX71885.1"
                     /translation="MSNSIAPSRPLAKLFTALFIWLPSSWASVLVLGLGVAVSMVAGD
                     LDAERQASEERSRVLNELALARARVEGALKATFNSTDGLVHLISLRGGIDPGLFADMA
                     RLAIGKNPHVRNITAAPDDTVTMVYPRTGNERVLGFRFADNPEQLSTVTLARERHHAI
                     LAGPVELVQGGRALIQRTPVFTLTGREVHYWGTVSIVAHIDGLLRTSDSPAIRLAIRG
                     KDALGAHGEMVDGDASVFASQPLLMDIEVPGGSWQLAAVPQHGWQTHPLYGSFYFHLG
                     LAISVLLALLAGARAEYGRQLRDHNRVLQNEMHERRRVEAALREEEERFRRLFDSSPD
                     PAWILEGPRFVKCNPAALSVFGYDAADSAVFSHPARLSPEYQPDGRASYTAAEAMMAM
                     ARARGSHRFEWLHLRSDGSPFYAEVTLTAMVMRGASLLHAVVRDISARKEAEQALFAN
                     RNLLQTLIESAGTVIYVFDTDGRLKLCNPQFETLCGRAREAMLGLRREAFLPLAIAGE
                     HAANDQQVMLSGAQREFEEHMVLDGRSHVFLSIKRPLLENGVVTGVVGISTDITERKA
                     AEEALRLYANIFEQTNEAILVTDRDNCIVRVNPALEHITGYRFDELRGRNPSMLASGN
                     TPGHVYHELWQALHTHGHWQGELWDRRKDGAVYPKWTNISVLRNAHGEITHYLGLFTD
                     ITERKHAEARIEHLAHHDKLTGLFNRHSLQERLGQALASATYANTRLAVLFIDLDRFK
                     IINDTLGHHIGDQLLVEVANRLRQVGHANDIVARLGGDEFVVVLASDESAREALPIAC
                     QMLAALGAPYLLDGNQLHSTPSIGISVYPDDGDSVDALMKAADTAMYHAKAQGRNNVQ
                     FFTAEMNAAAAERMELERDLHHAIAHQQLDVHYQPQVQAASGRVCGVEALLRWPHPTR
                     GQVSPLKFIPIAEESGLIEALGAWVLRQACQQVAHWKSIGIHGVRMAVNLSAHQLRSR
                     QLVDTVREAMHAHGVQAGELELEITESAVMKDPQAAIDTLQQLRDLGVHLAIDDFGTG
                     YSSLAYLKLLPVDVLKIDRSFVKDIETDRNDAAICASILALAKSLGLKVVAEGVETAA
                     QRDFLDSHGCDYLQGYFFAKPQPAALCAEQWRQAPALPAPVNP"
     gene            3929..4525
                     /locus_tag="PKN55_11365"
     CDS             3929..4525
                     /locus_tag="PKN55_11365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014087993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0010945 - CoA pyrophosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HNX71886.1"
                     /translation="MLPTHIDAAHDWLLTRLAHPLAERPGDVPHRQPNLTRPAAVLIP
                     LVWRPESPGIVLTRRESGLRHHAGQISFPGGKLEPDDPSPRAAALREAHEEIGLAPQQ
                     VQVVGELPEYVTVTGFLVTPVVGLIAPPLDLRPQAGEVAEIFELPLSLALDAGNYVRH
                     DYEQDGRRGQYLSLTHQERFIWGATAAMLRLLQAALSR"
BASE COUNT          812 a         1404 c         1487 g          839 t
ORIGIN      
        1 acgaatatca ggccatccac cacgcccacc cagatgaagc cgccgccctg ctaagccaag
       61 ccgaagcgct attgctgcca gccgaagcca gccagcccgg ccttgccgcc tacaatctgg
      121 cctgcatcgc cgcactgcgc cagcgccccg ccgacgccat ccattggcta cgctcctgcc
      181 aaacccacgg cactttgccg tccgaagagc acttccgcac tgactccaac ctcgacccga
      241 tccgcgacac gcccgagttc accgcctggt gggcggaggt gtttggccgg ggcgatgcgg
      301 ctatgggttg accggggcgg gcagggctgg ggcttgccgc cattgttcgg cgcagagggc
      361 ggcgggttgg ggtttggcga agaaatagcc ttgcagataa tcgcagccgt ggctgtccag
      421 aaaatcccgt tgggcggcgg tttctacgcc ttcggccacc actttcaggc ccaggctttt
      481 ggccagggcg aggatggagg cgcagatggc ggcgtcgttg cggtcggttt cgatgtcttt
      541 gacaaaagag cggtcgattt tcagcacgtc taccggcagc agtttcaggt aggccaggga
      601 ggagtagccg gtgccaaagt cgtcgatggc caggtgtacg cccaggtcgc gcagttgttg
      661 cagggtgtcg atggcggctt gcgggtcttt catcaccgcg gattcggtaa tttccagctc
      721 cagttcgccg gcttgcacgc cgtgggcgtg catggcttca cgcacggtgt ccaccaattg
      781 gcgcgagcgc agttggtggg cggatagatt gaccgccatg cgcacgccgt ggatgccgat
      841 ggatttccag tgcgccactt gctggcaggc ctggcgcagc acccaggcgc ccagggcttc
      901 gatcaggccg gattcttcgg caatcgggat gaatttcagc ggcgagactt gcccacgggt
      961 ggggtggggc cagcgcagca gggcttccac gccgcacacc cggccgctgg cggcttgcac
     1021 ttgcggctgg tagtggacat ccagttgctg gtgggcgatg gcgtggtgca ggtcgcgctc
     1081 cagctccatg cgctcggcgg cggcggcgtt catttcggcg gtaaaaaact gcacattgtt
     1141 tcgcccctgc gccttggcgt gatacatggc ggtgtcggcg gctttcatca gggcgtccac
     1201 gctgtcgccg tcgtcggggt agacgctgat gccgatgctg ggggtggagt gcagttggtt
     1261 gccgtccagc agataggggg cgcccagcgc ggccagcatc tggcaggcga tgggcagggc
     1321 ttcgcgggcg gattcgtcgc tggccagcac cacaacaaat tcgtccccgc ccaggcgggc
     1381 gacgatgtca ttggcgtggc ccacctggcg caggcggttg gcgacttcca ccagcagttg
     1441 gtcgccgatg tggtggccca gggtgtcgtt gatgatcttg aagcggtcca ggtcgataaa
     1501 cagcacggcc agccgggtat tggcgtaggt ggcgctggcc agcgcctggc ccaggcgctc
     1561 ttgcaggctg tggcggttaa ataggccggt gagtttgtcg tggtgggcca ggtgctcgat
     1621 gcgcgcttcg gcgtgcttgc gctcggtgat gtcggtaaac aggcccagat aatgggtgat
     1681 ttcaccatgg gcattgcgca gcacggaaat attggtccac ttggggtata ccgcgccgtc
     1741 cttgcgtctg tcccacagct cgccctgcca gtggccatgc gtgtgcaacg cctgccatag
     1801 ctcgtggtag acatgaccag gggtgttgcc cgaggccagc atgctggggt tgcgcccgcg
     1861 cagctcgtca aagcggtagc cggtgatgtg ttccagcgcc gggttcaccc gcacgatgca
     1921 gttgtcccgg tcggtgacca gaatcgcctc gttggtttgc tcaaagatat tggcgtacag
     1981 gcgcagggct tcttcggcgg ctttgcgctc ggtgatgtcg gtggaaatcc ccaccacgcc
     2041 ggttaccacg ccgttttcca gcagtgggcg tttgatactg agaaagacat gagatcggcc
     2101 atctaacacc atatgttctt caaattcacg ctgggcgccg ctgagcatca cttgctggtc
     2161 gttggcggcg tgttcgccgg caatggccag tggcaaaaac gcctcgcggc gcaggcccag
     2221 catggcttcg cgcgcgcggc cgcacagggt ttcaaactgc gggttgcaca gtttgagccg
     2281 gccatcggtg tcaaacacat aaatcaccgt gccagcgctt tcgatcaggg tttgcagcag
     2341 gttacggtta gcaaacagtg cttgctcggc ttccttgcgg gcagagatgt cgcgcaccac
     2401 ggcgtgcagc agcgaggcgc cgcgcatcac catggcggtg agggtgactt cggcataaaa
     2461 cggcgagcca tcgctgcgca ggtgcagcca ttcaaagcga tggctgccgc gcgcgcgcgc
     2521 catggccatc atcgcctcgg cggcggtata cgaggcgcgg ccgtcgggtt ggtattccgg
     2581 cgacaggcgc gccgggtggc tgaacaccgc actgtcggcg gcatcgtagc caaacacgct
     2641 cagggcagcc gggttgcatt tgacaaagcg cgggccttcc agaatccagg ccggatcggg
     2701 cgaggaatca aataagcggc gaaagcgctc ttcttcttcg cgcagcgcgg cttctacccg
     2761 gcggcgttcg tgcatttcgt tttgcagcac gcggttgtgg tcgcgcagtt ggcggccata
     2821 ttcagcgcgt gcgccagcca gcagcgccag cagcacgctg atggccaggc ccaaatgaaa
     2881 ataaaacgaa ccatacagcg gatgtgtctg ccagccgtgt tgcggcacgg cagccagttg
     2941 ccagctgccg ccaggcactt cgatatccat cagcagcggc tggctggcaa acacgctggc
     3001 atcgccatcc accatctcac catgcgcgcc cagcgcatcc ttgccgcgaa tggccaggcg
     3061 gatggccggc gagtcgctgg tgcgcagcag gccgtcgata tgggcgacaa tcgacaccgt
     3121 gccccagtaa tgcacctcgc ggccagtcag ggtaaacacg ggtgtgcgct ggatcagcgc
     3181 gcggccaccc tgcaccagct ccaccggccc ggccaggatg gcgtggtggc gctcgcgcgc
     3241 cagggtcacc gtgctcaatt gctcggggtt gtcggcaaaa cgaaagccca gcacccgctc
     3301 gttgccagta cgtggataga ccatggtcac cgtgtcatcc ggggcggcgg tgatattgcg
     3361 cacatgcggg tttttgccaa tcgccagcct ggccatgtcg gcaaataagc cagggtcgat
     3421 gccgccacgc agtgagatca ggtgcaccag gccatcggtg gagttaaacg tggctttcag
     3481 ggcgccttct acccgcgcgc gcgccagcgc cagttcgttg agcacgcggc tgcgttcttc
     3541 gctggcttgc cgctcggcat ccagatcacc ggccaccatg ctgacggcca cccccaggcc
     3601 cagcaccagc acagaggccc aggacgatgg caaccagata aacagcgcag tgaacaactt
     3661 ggcaagcggc cgagatgggg cgatcgaatt gctcaagagg cggcccccat ggcagacaag
     3721 gtgaaatcag gcatcacagt gaagaaaaag catggctgac aatagctaca ctatagccca
     3781 tatgtaagcc agctaaaagt aaaactacgg gattctacct gcctgcacct ggccaccttt
     3841 gatgcaaatc aaatacccgc accggccgtc tggcaaacac gttacactga aagctggatc
     3901 atccgtttgt tgcccaccgc ccgaacccat gctgcccacc catatcgacg ccgcccacga
     3961 ctggttgctg acccgcctgg cccacccact ggccgaacgc cctggcgatg tgccacaccg
     4021 ccaacccaac ctcacccgcc cggccgccgt gttgattccg ctggtctggc gaccagaaag
     4081 ccctggcatt gtgcttaccc gtcgggaaag cggcctgcgc caccatgccg ggcagatcag
     4141 cttcccaggc ggcaagctgg agccagacga ccccagccca cgcgcggcgg cgctgcgcga
     4201 ggcgcacgaa gaaatcggcc tggcgccgca acaggtgcaa gtggtgggtg aactgcccga
     4261 gtatgtgacg gtgacgggtt ttttggtgac gcccgtggtt gggctgattg ccccgccgct
     4321 ggatttgcgc ccacaggcgg gcgaggtggc ggagattttt gaactgccgc tgtcgctggc
     4381 gctggacgcc ggcaattatg tgcgccacga ctacgaacaa gacggccgac gcggacaata
     4441 tctgtcgctc acccatcagg agcgttttat ctggggggcg acggcggcca tgctgcggct
     4501 gttgcaggcg gccttgagtc gctgagggtc aaggcgtggg ct
//
LOCUS       DAOPYX010000247         4539 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_24528_length_4539_cov_10.285013, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000247 DAOPYX010000000
VERSION     DAOPYX010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 4539)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4539)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4539
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24528_length_4539_cov_10.285013"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1659
                     /locus_tag="PKN55_11370"
     CDS             <1..1659
                     /locus_tag="PKN55_11370"
                     /inference="COORDINATES: protein motif:HMM:NF022062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIPR family protein"
                     /protein_id="HNX71887.1"
                     /translation="DQRLAGVQAEIATRAADELNLHDPGRLKSLAFVYLCVQTLLDLD
                     MEQAFDCLTEGGGDFGVDALHMSEEVDGEFVVTLFQSKYRHKLDATGHFEEKGIEALI
                     KAIRHIFNPAAELGQINERLRIKVEAVRSMIRDGWIPRVRAVACSNGQRWNASAQQAI
                     EREGFGEQVSWEHLNHDGLLALWQRLKPVNETLRLVGKAVVEDMNFSRVCLGRLPVTE
                     VAALMRIHGERLLERNIRRYLGLHGNRVNEGIQATLRSHSPADFYFFNNGLTVVCDDF
                     AYNALQGSDYQIKVDNLQIVNGGQTCMTILKTEQELNKLGQHLPADASVLVRIYKLPK
                     DNEDLVLQITHATNSQNPVDLKDLRANDARQQQLEHSLRDLGFTYRRKRMEGASKAGD
                     ISSGQAAEAILAVWRHAPHQARFLTRAHFGKLYETIFTEQLNGAQTIIAVLLHRLADQ
                     RRRRPAEDDPLCVRYAACFIAMQMGRRLLAELGISLAALDHRHFAQAKTLLESKAQIY
                     FDASVADVERALAQLYGGQPVSLQQLAATFRRGDVIDTLLGMPV"
     gene            complement(1691..3046)
                     /locus_tag="PKN55_11375"
     CDS             complement(1691..3046)
                     /locus_tag="PKN55_11375"
                     /inference="COORDINATES: protein motif:HMM:NF005453.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HNX71888.1"
                     /translation="MPQVFSPARVAALTERARADYIAQRPRSAALAAGSGHLLSGVPM
                     HWMRDWGTPFPLYIDHAQGAQCVDVDGHVYTDFCLGDTGAMFGHSPAPVAAAIAERAA
                     RGYSCMLPGADANWVGAHLAERFGLPVWQIATTATDANRFVLRWARGLTGRPVVVVFD
                     GCYHGTVDDTLVRQDADGRTVARSGLVGQVADLGQYTRVVPFNDLAALHAALAPGDVA
                     CVLTEPALTNIGMVLPAEGFLAGVRQLTRQHGALLVIDETHTISTGYAGYSGQIGLEP
                     DMLVLGKPIAGGLPAAVYGMSAEVAARAEALLASKPDGHSGMGTTLSANLFTLAAMRA
                     NLEQVMTRDAYALTTPRAARLADALRALFARHRLPWCVTQIGARCEFQFCPTPPRTGA
                     EAEAAMDHALERLIHLSLLNQGVLITPFHNMMLVCPDTPETAIAALVDGVEAVLEALR
                     G"
     gene            complement(3081..3848)
                     /locus_tag="PKN55_11380"
     CDS             complement(3081..3848)
                     /locus_tag="PKN55_11380"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014088040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HNX71889.1"
                     /translation="MCQLLGMNCNTPTDILFSFEGFHRRGGLTDQHADGFGIAFFEGV
                     GCRLFLDDQASAHSPVSDLIRRYPIKSKNVIAHIRKATQGRVALENTHPFHRELWGRN
                     WIFAHNGNLEHFAPARGQYYRPVGDTDSEAAFCHLLETLRQRFAEPPPLAELTAAVRE
                     LAASIRAHGVFNFMLSNGDVLFTHCSTHLHYLVRQAPFSVAHLVDNDVSIDFAEVTTP
                     SDRVAVIATQPLTDNETWTAHPKDTVLVFRDGAPLMG"
     gene            3954..4523
                     /locus_tag="PKN55_11385"
     CDS             3954..4523
                     /locus_tag="PKN55_11385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018748027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNX71890.1"
                     /translation="MTTITVARKGREVAIAADSQSTFGDTRLTSTYDARYNKIFQLDD
                     SFIGISGSAAHDLVFQSALSKLKNRDFSSRAAVFDTFRKLHPKLKEDFFLKPDEEEND
                     PYESSQMTVLIANPSGIFAVYSMREVYEYTRFWAIGSGRDYALGAMYAAWNTLDTAAA
                     VAQLGVEAGCEFDINTAAPLTLYSTTLRD"
BASE COUNT          844 a         1407 c         1483 g          805 t
ORIGIN      
        1 gatcagcgtt tggccggtgt gcaggccgaa atcgccacgc gcgcggcgga tgagctgaat
       61 ctgcacgacc ctggccggct gaaatcactg gcgtttgtct atctgtgtgt gcaaaccctg
      121 ctggacctgg acatggagca ggcctttgac tgcctgaccg aggggggcgg ggattttggc
      181 gtggatgcct tgcacatgag cgaagaggtg gatggcgagt ttgtggtcac cttgtttcaa
      241 agcaaatacc gccacaagct cgacgcgacc gggcattttg aagaaaaagg cattgaggcg
      301 ctgattaagg ccattcgcca tatttttaac ccagcggccg agctggggca aatcaacgaa
      361 cgcttgcgca tcaaagtaga agcggtgcgt tcgatgattc gcgatggctg gattccgcgt
      421 gtgcgcgccg tggcgtgcag caacggccag cgctggaatg ctagcgcgca acaggccatt
      481 gagcgcgagg gctttggcga gcaggtcagc tgggagcatc tcaaccatga cggcctgctg
      541 gcgctgtggc agcggctcaa gccggtgaat gaaaccctgc gcctggttgg caaggccgtg
      601 gtggaagaca tgaacttcag ccgggtgtgc ctgggacgtt tgccggtgac cgaggtggcg
      661 gcgctgatgc gcatccacgg cgagcgtctg ctggagcgca atatccgccg ctatctgggt
      721 ctgcatggca accgggtgaa tgaaggcatt caggccacct tgcgctctca cagcccggcg
      781 gacttttatt ttttcaataa cggcctgacc gtggtctgcg acgattttgc ctataacgcc
      841 ctgcagggca gcgattacca gatcaaggtg gacaatctgc aaatcgtcaa cggcggccaa
      901 acctgcatga ccattcttaa aaccgagcaa gagttaaaca agctcggcca gcacttgccg
      961 gcggacgcca gcgtgctggt gcgcatttac aagctgccca aggacaacga agacctggtg
     1021 ctgcaaatca cccacgccac caacagccaa aacccggtag accttaaaga cctgcgcgcc
     1081 aatgacgcca gacagcagca attggagcat agcctgcgcg atctgggttt cacgtatcgg
     1141 cgcaagcgca tggaaggcgc aagcaaggcg ggagatattt cttccgggca ggccgccgaa
     1201 gccattctgg cggtgtggcg gcatgcgccg caccaggcgc gctttttgac gcgggcgcat
     1261 tttggcaagc tgtatgagac gattttcacc gagcaactga acggcgcaca aaccattatc
     1321 gccgtcttgc tgcaccgctt ggccgaccaa cgccgccgcc gcccagccga ggatgatccg
     1381 ctgtgcgtgc gctacgccgc gtgctttatc gccatgcaga tggggcgccg cctgctggcg
     1441 gaactgggta tttcacttgc cgcgctggat caccgccact ttgcccaggc caagaccttg
     1501 ctggaaagca aggcgcagat atatttcgac gccagtgtgg ccgatgtcga gcgggcgctg
     1561 gcccagctgt atggcgggca gccagtatcg ttgcaacaac tggccgccac tttccggcgc
     1621 ggggatgtga tcgacaccct gttgggcatg ccggtgtgag cagcggcctt gccgccgcac
     1681 atcaagctgc ttacccccgc agcgcctcca gcactgcttc caccccatcc accagcgcgg
     1741 cgatcgccgt ttccggcgtg tccgggcaca ccagcatcat gttgtgaaac ggggtgatca
     1801 gcacgccctg attgagcagg ctcaggtgaa tcaagcgctc cagcgcgtgg tccatggctg
     1861 cttccgcctc ggcgccggtg cgcggcgggg tggggcaaaa ctggaattcg cagcgcgcgc
     1921 cgatttgggt gacgcaccag ggcaggcgat ggcgggcaaa cagcgcgcgc agggcgtcgg
     1981 ccaggcgggc ggcgcggggc gtggtgaggg cgtaggcgtc gcgggtcatc acctgctcaa
     2041 gattggcgcg catggcggcc agggtgaaca gattggcgct gagcgtggtg cccatgccgc
     2101 tgtggccatc cggcttgctg gccagcagcg cctcggcgcg ggcggccacc tcggcgctca
     2161 tgccgtacac cgccgccggc agcccgccgg caatcggttt gcccagcacc agcatgtctg
     2221 gctccaggcc gatctggccg ctgtagccgg cgtagcccgt gctgatggtg tgggtttcgt
     2281 cgatcaccaa cagtgcgcca tgctggcggg tgagctggcg cacgcccgcc aaaaagccct
     2341 cggccggcag caccatgccg atattggtca gcgccggctc ggtgagcacg caggccacgt
     2401 cgccgggggc cagcgccgcg tgcagcgccg ccaggtcgtt aaacggcacg acgcgggtgt
     2461 attgccccaa atccgccacc tggcccacca gcccgctgcg ggcgacggtg cggccgtcgg
     2521 cgtcttgtcg caccagggtg tcgtccaccg tgccgtgata acagccatca aacaccacca
     2581 ccaccggccg gccggtcaag ccgcgcgccc agcgcagcac aaagcggttg gcgtcggtgg
     2641 ccgtggtggc gatttgccac accggcaggc caaagcgctc ggccagatgc gcgcccaccc
     2701 agttggcgtc ggcgccgggc agcatgcagc tgtagccgcg cgcggcgcgc tcggcaatgg
     2761 cggcggccac cggcgcgggc gagtggccaa acatcgcgcc cgtgtcgccc agacaaaagt
     2821 cggtgtagac gtggccatcc acatccacgc actgcgcgcc ctgggcatga tcgatataca
     2881 gcggaaacgg cgtgccccag tcgcgcatcc agtgcatggg cacgccagac agcaaatgcc
     2941 cgctgccggc ggccagcgcc gccgagcgcg ggcgctgggc gatatagtcg gcgcgggcgc
     3001 gctcggtcag cgcggccacg cgcgcggggg aaaacacctg cggcatgctt ggggctcctg
     3061 tgggtggggc tgaccgacgc ttagcccatc aacggcgcgc catcccggaa caccagcacg
     3121 gtgtctttcg ggtgcgccgt ccaggtttca ttatccgtca gcggttgtgt ggcaatcacc
     3181 gccacccggt cgctgggggt ggtgacttca gcaaagtcga tgctcacgtc gttgtccacc
     3241 aggtgcgcca cgctaaacgg cgcttgtcgc accaggtagt gcaggtgggt gctgcaatgg
     3301 gtgaacagca cgtcgccatt gctgagcatg aagttgaaca caccatgggc gcggatgctg
     3361 gcggccagtt cgcgcacggc ggcggtcagc tcggccagcg gcggcggttc ggcaaagcgc
     3421 tggcgcaggg tttccagcag gtggcaaaac gcggcttcag agtcggtgtc gcccactggc
     3481 cggtaatact ggccgcgcgc cggggcaaag tgctccagat tgccattgtg ggcaaaaatc
     3541 cagttgcgcc cccacagctc gcggtgaaac gggtgggtgt tttccagcgc cacccggccc
     3601 tgggtggcct tgcggatgtg ggcgatgacg tttttggatt tgatcgggta gcggcggatc
     3661 aaatccgaca ctggcgagtg cgcgctggcc tggtcgtcca gaaacagccg gcagcctacg
     3721 ccttcaaaga aggcgatgcc aaagccgtcg gcatgctggt cggtcagccc gccgcgccgg
     3781 tgaaagccct caaaagaaaa caggatgtcc gtcggggtgt tgcagttcat gcccagcagc
     3841 tggcacatgg tgtgacctca tggatgcgcc gccggccatg ctcggccatt ggcttgcttt
     3901 ttgcccaaat ggctttatct tacgccttat tgccttcagg aaaaaaccaa gccatgacca
     3961 ccatcaccgt ggcgcgcaag gggcgggaag tggcgattgc cgccgacagt cagtccacat
     4021 ttggcgacac ccggctgact tccacttacg acgcccgcta taacaagatt tttcagctgg
     4081 acgacagctt tatcggcatt tccggcagcg ccgcgcacga cctggtgttt cagtcggcgc
     4141 tgagcaagct gaaaaaccgt gatttttcca gccgtgcggc ggtgttcgac acttttcgca
     4201 agctgcaccc caagctgaaa gaagattttt ttctcaagcc ggacgaagaa gaaaacgacc
     4261 cttacgaatc cagccagatg acggtgctga tcgccaaccc cagtgggatt tttgccgtgt
     4321 attcgatgcg cgaggtgtac gaatacaccc gcttctgggc gattggctcg gggcgcgatt
     4381 acgcgctggg ggcgatgtac gccgcctgga acacgctgga caccgccgcc gccgtggccc
     4441 agctgggggt ggaggccggt tgcgagtttg acatcaacac cgccgccccg ctgactttgt
     4501 attccaccac gctgcgcgac taagcgccgc tcaccacac
//
LOCUS       DAOPYX010000248         4524 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_24650_length_4524_cov_4.953681, whole genome shotgun sequence.
ACCESSION   DAOPYX010000248 DAOPYX010000000
VERSION     DAOPYX010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 4524)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4524)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4524
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24650_length_4524_cov_4.953681"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            129..830
                     /locus_tag="PKN55_11390"
     CDS             129..830
                     /locus_tag="PKN55_11390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011133954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HNX71891.1"
                     /translation="MNASLGLAHFWAQGDAVSHTVALALLLMSVWSWTVMLGKVWAHW
                     RVRHTVRVAAPAFWAAPSREAALTALRAEDASGVFAGLAQEAEQAARAYEQQRAGGGI
                     GAGVDASEFIGRSLQQALTHAQARLESGLTFLASVGATAPFVGLFGTVWGIYHALIGL
                     SGASRMSIDQVAGPVGEALVMTAAGLFVAIPAVLAYNACTRANRLTLAHLDGFAHGLH
                     ALLAAGVRVTPNARA"
     gene            849..1262
                     /locus_tag="PKN55_11395"
     CDS             849..1262
                     /locus_tag="PKN55_11395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014088737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HNX71892.1"
                     /translation="MAFSSFSRQGGHPPPLAEINTTPLVDVMLVLLVIFLITAPVLQQ
                     AVVLDLPQASAAPLPAPPRVVRVALNAGGQVFWDDAPLPHEQLAAKLAAVAADQPELH
                     LRADRHTPYEAVAGLIACAQQAGLVKIAFVTEPAP"
     gene            1710..3152
                     /locus_tag="PKN55_11400"
     CDS             1710..3152
                     /locus_tag="PKN55_11400"
                     /inference="COORDINATES: protein motif:HMM:NF012573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HNX71893.1"
                     /translation="MATTISSSTSYTLGAGEDNLTLLGTSNINGAGKSGANVLTGNSG
                     ANILSGFAGNDTLYGGAGDDVLYGHSAGGSGPQDNDMLYGEDGNDTLYGDGGDVLFGG
                     NGADTYYLTGAGNSISDMGADTGIDVVKSWTSVNSFNYFNTSQPYSIVSDGVERIELQ
                     GSANLTAGGSNGNDALYGNTGNNILVGAAGADSLYGGAGNDTLYANNASNPLADDGVR
                     DFLFGGDGNDILYGSGTDLLMGDAGDDVYYVSGDNVTVSDYAGFDTVYASGSFTSGFS
                     GGVDVIQLTGSLSSSATGDFGANLIKGNIGANTLNGGDGADTLDGGGGVDILKGDGGN
                     DTLIIHANNGSSADQLGATLTGGAGNDVFWIAAGYSGNHTVGTTAVTLTDFTQGIDQI
                     RLSIAPGLTAPSSITTLTASPTDTLGSLLAQAVHQTTTYTQPALSQFVWQGETYLVLD
                     TTADPTWAGSDLAIKLAGAPTLTLANFSFA"
     gene            3424..4461
                     /locus_tag="PKN55_11405"
     CDS             3424..4461
                     /locus_tag="PKN55_11405"
                     /inference="COORDINATES: protein motif:HMM:NF012573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX71894.1"
                     /translation="MAIIITASTDYTLGAGEDNLQLTGSADVNGTGNAGNNFLTGNDG
                     ANVLIGGAGGDFLLGGAGDDILIGNVDSVTADAEADYLNGEDGNDIIYLTGRDMTDSG
                     AGADTYWVMTGRNNHIWDTGTDTSVDVVKTYTSVTMGSFNFDTSKPYAMIYDGIERVE
                     LQGTANLSAVGGLGNETLIGNSGNNLLDGGGGVDVLDGGAGNDTLCLDSGPAAQQGAT
                     LTGGDGNDVFRFQPGYSGDHSQGADSVVITDFTQGADILSFGLPAGIAAPSAIHTLTV
                     QAGDTLASLLTQAIHQTTTYTQPALSQFVLDGDTYLVMDTTADPTWAASDLAIKLTGA
                     HTLSMTDISFG"
BASE COUNT          882 a         1352 c         1380 g          910 t
ORIGIN      
        1 atgcctttct tgcctgaccg acacacgcct ggccaggaag gctttgctgc gtgttgtgcg
       61 tggtgcactg tcttgtcgag ccaaacgcct ggccgttttt gttttcagtt acccgtaagg
      121 aaatctgcat gaatgcttct cttggattgg cccatttctg ggcgcaaggc gacgcggtgt
      181 cgcatacggt ggcgctggcg cttttgctga tgtcggtgtg gagctggacg gtgatgctgg
      241 gtaaggtgtg ggcgcactgg cgtgtgcgcc acacggtgcg ggtggcggcg ccggcttttt
      301 gggcggcgcc gtcgcgtgag gcggcgctga ccgccctgcg cgccgaagac gccagcgggg
      361 tgtttgccgg tttggcgcag gaagctgaac aggcggcgcg cgcctacgag cagcagcgcg
      421 cgggcggcgg catcggcgct ggggtggacg ccagcgaatt catcggccgc agcttgcaac
      481 aggcgctgac ccacgcccag gcgcggctgg aatccggcct gacctttctg gcgtcggtgg
      541 gcgccacggc gccgtttgtc ggcttgtttg gcacggtgtg ggggatttat cacgcgctga
      601 tcggcctgtc tggcgcatcg cgcatgagca ttgatcaagt ggccggcccg gtgggcgagg
      661 cgttggtgat gaccgccgcc ggcttgtttg tcgccattcc ggcggtgttg gcctacaacg
      721 cctgcacccg cgccaaccgg ctgacgctgg cgcatctgga tggctttgcc catggcctgc
      781 atgcgctgct ggctgccggc gtgcgtgtca ccccgaatgc gcgcgcctga acaccaggag
      841 actgagccat ggctttttca tcgttttccc gtcagggcgg ccacccgccg ccgctggccg
      901 agatcaacac cacgccgctg gtggatgtga tgctggtgct gctggtgatt tttttgatca
      961 ccgcgccggt gttgcagcag gcggtggtgc tggatttgcc gcaggccagc gccgcgcctt
     1021 tgcccgcccc gcctcgggtg gtgcgtgtgg cgctgaacgc cggggggcag gtgttctggg
     1081 acgatgcgcc gctgccgcac gagcagctgg ccgccaagct ggccgccgtg gcggccgatc
     1141 aacccgagct gcatttgcgc gccgaccgcc atacccccta tgaggccgtg gccggcctga
     1201 tcgcctgcgc ccagcaggcg gggctggtga agattgcctt tgtcactgag cccgcaccct
     1261 gagtgttgcc tgcggacatt gttttttcag ccatgccgtg ccggagtcat cctggcgcgg
     1321 catttttgtt taacgcataa attatgccat tcagttacca gttggtaact gtcgcaaaaa
     1381 acagacatgc caatcggcat tgatatgttt tatgcagatg aaaaatttgc cggttgatgc
     1441 ccgtgattca cagcctgaca agcacttgtt tcaccagcgg tatttgtaat aacaatttac
     1501 attacactcg gcgccggcaa tttttgccac ccaactcggc aaacatttac cccgtatttc
     1561 tacgtaatta ctgtgcatgg tcccgcaggg cggtccgcat gtaaaaaaac aaatgtcatt
     1621 tttgctctct ccccttgtct ggcgtgtgtg aagttaatta actttgtagg gcgagctttc
     1681 gcatatccgc gaatcgaaag gaaaacatca tggcaaccac catcagcagc agcaccagct
     1741 acaccctggg cgcaggcgaa gataatctga ccttattggg cacatccaat atcaatggcg
     1801 cgggcaagag cggcgccaat gtgctgaccg gcaacagcgg cgccaatatt ctgtccggct
     1861 ttgccggcaa tgacaccttg tatggcggcg ccggcgatga tgtgctgtac ggccattccg
     1921 ccggcggcag cggcccgcaa gacaatgata tgttgtatgg cgaagatggc aacgacaccc
     1981 tgtacggtga cggcggcgat gtgctgtttg gcggcaatgg cgctgacact tactacctga
     2041 ccggcgcggg caatagcatc agcgacatgg gcgctgacac cggcattgat gtggtgaaaa
     2101 gctggacctc ggtcaattcg ttcaactatt tcaacaccag ccaaccctac agcatcgtct
     2161 cggacggcgt tgagcgcatt gagctgcaag gcagcgccaa cctgaccgca ggcggcagca
     2221 atggcaatga cgcattgtat ggcaacaccg gcaacaatat tttggtgggc gccgccgggg
     2281 cggattcgct gtacggcggc gccggcaacg atacgctgta cgccaacaat gccagcaacc
     2341 cactggcaga cgatggcgtg cgcgactttt tatttggcgg cgatggcaac gacatcctgt
     2401 acggctcggg cactgacctc ctgatgggtg acgccggtga cgatgtgtac tacgtgagcg
     2461 gcgacaatgt tacggtcagt gattacgcgg gctttgacac ggtgtatgcc tctggttcgt
     2521 tcacctccgg cttctccggc ggcgttgatg tgattcagct cacaggctcg ttgagcagca
     2581 gcgctacggg tgattttggc gccaatctga tcaagggcaa tatcggcgcc aacaccctca
     2641 acggcggcga tggcgccgac acgttggatg gcggcggcgg ggtggatatc ctcaagggcg
     2701 atggcggcaa cgacactctg atcatccatg ccaacaatgg cagctcggcc gaccaactgg
     2761 gcgccacgtt gaccggcggc gccggcaacg atgtgttctg gattgctgcg ggttactctg
     2821 gcaatcacac ggtgggaacc acggcagtca ccctcaccga ctttacccaa ggcattgacc
     2881 agattcgcct gagcattgcg cctggcctga cggcgccgtc gtcgatcacc accctgacgg
     2941 caagccccac cgacacgctg ggcagcctgc tggcccaggc ggtgcatcaa accaccacct
     3001 acacccagcc ggcgctgagc cagtttgtct ggcaaggcga aacctatctg gtgctggaca
     3061 ccaccgccga cccgacttgg gcaggctcgg atctggccat caaactggct ggcgcgccga
     3121 cgttgaccct ggcgaatttc agctttgcct gattgtccgc acatgacatg agtcaggccg
     3181 cgcggcaata ttgcgcagcc atgtcaaccg gaaggccagt ggccttccgg tttttgtgct
     3241 gttctttctg cgctcagaga aaatttcatc acgaaaaaac agttaacatt cacaaccctc
     3301 gcccaaaatc ccctgagcaa gttacctggc agtaaccgta ttcgcacgca ttgcgagcca
     3361 ttgttattta aattaacttt acctggcgaa tgttcgcacc ttcacgaaga agaggattaa
     3421 atcatggcga tcatcatcac cgcttcgacc gactacaccc tgggcgcagg ggaagacaat
     3481 ctgcaactca ctggctcggc tgatgtaaat ggcacaggga atgcaggaaa caatttcctc
     3541 accggcaacg atggcgccaa tgtgctgatc ggcggcgcag ggggagattt cctgctgggg
     3601 ggtgccggcg atgacatcct gattggcaat gttgacagcg tgactgcaga tgctgaggcg
     3661 gactacctga acggtgagga cggcaatgac atcatttatc tgactggccg tgatatgacc
     3721 gatagcggcg ctggcgcaga cacctattgg gtgatgacgg gccgcaacaa ccacatctgg
     3781 gataccggca ccgacaccag cgtggacgtg gtcaagacct acacctcagt gaccatgggc
     3841 agcttcaatt tcgacaccag caagccgtac gccatgatct acgacggcat tgagcgcgtc
     3901 gagctgcaag gcacggccaa tctgagcgca gtgggcgggc tgggcaacga aaccctgatc
     3961 ggcaacagcg gcaacaatct gctggatggc ggcggcgggg tggatgtgct ggacggcggc
     4021 gccggcaacg acaccttgtg cctggacagc ggcccggccg ctcagcaagg cgccacgctg
     4081 accggcggcg acggcaatga tgtgttccgc ttccagccgg gttacagcgg cgaccacagc
     4141 cagggcgccg atagcgtggt gatcaccgac tttacccaag gcgcggacat cctcagcttt
     4201 ggcctgcccg ccggcattgc cgcgccgagc gccattcaca ccctcaccgt gcaggccggc
     4261 gacacgctgg ccagcctgct gacgcaggcc atccatcaaa ccaccactta cacgcaaccg
     4321 gcgctgagcc agttcgtgct ggatggcgac acctacctgg tgatggacac caccgccgac
     4381 ccgacctggg cagcgtctga cctggccatc aagctgactg gcgcccatac gctgagcatg
     4441 acggacatca gcttcggcta agagccgcta acacaccctg acagataagg catacatcat
     4501 gaccacctct attagcgctt ctac
//
LOCUS       DAOPYX010000249         4498 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_24890_length_4498_cov_9.401080, whole genome shotgun sequence.
ACCESSION   DAOPYX010000249 DAOPYX010000000
VERSION     DAOPYX010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 4498)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4498)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4498
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24890_length_4498_cov_9.401080"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(2..1315)
                     /gene="ahcY"
                     /locus_tag="PKN55_11410"
     CDS             complement(2..1315)
                     /gene="ahcY"
                     /locus_tag="PKN55_11410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014085972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HNX71895.1"
                     /translation="MNAVVDLKTFTDYKVADISLAEWGRKEIAIAETEMPGLMALREE
                     YKDQKPLTGARIVGCLHMTIQTAVLIETLVDLGASVRWSSCNIFSTQDQAAAAIAAAG
                     IPVFAWKGETEEEFWWCIEQTLRGPDGWTPNMILDDGGDVTAIMHSKYPEMLDEVRGL
                     SEETTTGVHRLYDMVKKGELKVPAFNVNDSVTKSKFDNLYGCRESLVDGIKRATDVMI
                     AGKIAVVCGYGDVGKGSAQSFRGLGATVWVTEIDPICALQAAMEGYRVVTMDEAAALG
                     DIFVTATGNVNVITHAHMLAMKDQSIVCNIGHFDSEIDVAGLRQYTWENIKPQVDHII
                     FPDGKRIILLAEGRLVNLGCATGHPSFVMSASFTNQVIAQIELWQNPGKYAIDVHVLP
                     KHLDEKVARLHLKKIGAKLTELSDEQASYLGLDKTGPYKPTHYRY"
     regulatory      complement(1382..1453)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01057"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="S-adenosyl-L-homocysteine riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="S-adenosyl-L-homocysteine"
                     /db_xref="RFAM:RF01057"
     gene            complement(1565..2734)
                     /gene="metK"
                     /locus_tag="PKN55_11415"
     CDS             complement(1565..2734)
                     /gene="metK"
                     /locus_tag="PKN55_11415"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012696064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HNX71896.1"
                     /translation="MSEYLFTSESVSEGHPDKVADQISDAILDAILAQDKYARVAAET
                     LVNTGLVVLAGEITTHANIDYMGIARETIRRIGYDSSDLGFDAKGCAVLVAYDKQSPD
                     IAQGVNEGEGLDLDQGAGDQGLMFGYACDETPTLMPFPIYYAHRLVQRQSELRKDGRL
                     PWLRPDAKSQITCVYDSETGLPKAIDTVVLSTQHTPEIDHKTLTEAVIEDIIKPVLPA
                     HMLTADTKYLINPTGRFVIGGPMGDCGLTGRKIIVDTYGGAAPHGGGAFSGKDPSKVD
                     RSAAYAGRYVAKNIVAAGLARMCQVQVSYAIGVANPTSISVDTFGTGKLPNEAIVELV
                     KKHFDLRPKGIVQMLDLLRPIYSKTAAYGHFGREEPEFSWEATDKAAALRADAGL"
     gene            2803..3705
                     /locus_tag="PKN55_11420"
     CDS             2803..3705
                     /locus_tag="PKN55_11420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008953436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNX71897.1"
                     /translation="MSRLLIAVFSILSHLPLAVLQAVGVALGWLVYLSSPRYAARMRE
                     NLGQSGLATDAASQRRLLRQAVSATGMGALELVIAWGRSPTSIARLVKNCNNWHIVEE
                     ALAQNKGIIFITPHLGNYDIAGRYLSQRLIKRPQAIQLMAMYRPPKMAWLEPLMNAGR
                     VREGGLTAPATAAGVRMVMKTLKARQTTIILPDQVPGQGDGVWAPFFGRPAYTMTLAP
                     RLAQMDGVEVILFFGRRLGWGRGFEVEIARLPEPFTGDKTTDGAKLNQAVEQLIRQAP
                     AQYLWSYNRYKTPAGVAPAETPNP"
     gene            3702..>4498
                     /locus_tag="PKN55_11425"
     CDS             3702..>4498
                     /locus_tag="PKN55_11425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011134516.1"
                     /note="Acylates the intermediate (KDO)2-lipid IVA to form
                     (KDO)2-(lauroyl)-lipid IVA; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A biosynthesis lauroyl acyltransferase"
                     /protein_id="HNX71898.1"
                     /translation="MMTRFALAVLWLLHWLPVPVLAVLARVLGWLGYLLARERRRVGL
                     INLQRCFPDMSPSARRRIILRHCQHLMQSLLEYGIAWWGSPARLRRLVTLKNLHHLTD
                     QAEHNTIILYPHFIAFELCVFRLNLEQALVSVYSNQKNPVLNKQLYQGRSRFNNAKIV
                     SRQEGLRSIIKAMRDHTPFLYLPDQDFGPRDSVFVKFFAEDAATITGLSRISALAKAK
                     VVPAMARRVGFRYELEFYPAWENFPTDDVMADTRRMNAFIEERVREDP"
BASE COUNT          786 a         1353 c         1415 g          944 t
ORIGIN      
        1 cttagtagcg gtagtgcgtc ggcttgtacg ggccggtctt gtccagaccc agatagctgg
       61 cttgctcatc gctgagctcg gtcagcttgg cgccgatttt cttcaggtgc agacgggcaa
      121 ccttttcgtc caggtgcttg ggcagcacat gcacgtcaat ggcatactta cccggatttt
      181 gccacagttc gatctgggca atcacctggt tggtgaacga ggcgctcatc acgaacgacg
      241 ggtggccggt ggcgcagccc aggttcacca gacggccttc ggccagcagg atgatgcgct
      301 tgccgtccgg gaagatgatg tgatcaactt gcggcttgat gttttcccaa gtgtactgac
      361 gcaggccagc cacgtcgatt tcgctgtcga agtgaccgat attgcagaca atcgactggt
      421 ctttcatcgc cagcatatgg gcgtgggtga tcacattgac gttgccagtg gcggtgacaa
      481 aaatatcgcc cagcgccgct gcttcgtcca tggtcaccac gcggtagcct tccatggcgg
      541 cttgcagtgc gcagatcggg tcgatttcgg tcacccacac ggtggcgccc aggccacgga
      601 aggattgcgc cgagcccttg cccacgtcgc cgtaaccaca caccacggcg attttgccgg
      661 cgatcatcac gtcggtggcg cgcttgatgc cgtccaccag cgattcgcgg cagccgtaca
      721 ggttgtcaaa cttggacttg gtcaccgagt cattgacatt gaacgccggg actttcagct
      781 cgcccttctt gaccatgtcg tacaggcgat gcacgccggt ggtggtttct tccgacaggc
      841 cgcgcacctc gtccagcatt tccgggtact tgctgtgcat gatggcggtg acgtcgccgc
      901 catcgtccag aatcatgttc ggcgtccagc cgtccgggcc gcgcagggtt tgctcgatgc
      961 accaccagaa ttcttcttcg gtttcaccct tccaggcaaa caccggaatg cccgcagcgg
     1021 cgatggcggc ggcggcttga tcctgggtgg agaaaatatt gcacgaggac caacgcaccg
     1081 atgcgcccag atccaccagg gtttcgatca gcacggcggt ctggatggtc atgtgcaggc
     1141 agccgacgat gcgggcgccg gtcagcggct tttggtcttt gtattcttcg cgcagcgcca
     1201 tcaggccggg catttcggtt tcggcgatgg cgatttcctt gcggccccat tcggccaggg
     1261 aaatatcggc gactttgtag tcagtgaagg tcttcagatc gaccacggcg ttcatagcaa
     1321 ttccttacgc ggtggccggg cgggatgctc acctgtgcgt gcataccggg cccggcatgg
     1381 gttcaggtga gcgcagttcg aatccgagcc tgggggcggc cttgtgagcc acgccttgca
     1441 gcgctcctcg gaagattggg acaggattat acgggatttt gctggtgaac gccggatttt
     1501 gccggcgttc acccattgcg ctcatacact gacgagcggc aggcttaatt acaacgatga
     1561 cagattacag cccggcgtcc gcgcgcagcg cggcagcctt gtcagtggct tcccagctga
     1621 actccggctc ttcgcggcca aagtggccgt aagcggcggt cttgctgtag atcgggcgca
     1681 gcaggtccag catctgcaca atgcccttcg gacgcaggtc gaagtgtttc ttcaccagct
     1741 cgacaatcgc ctcgttcggc agcttgcccg taccaaaggt gtccaccgaa atcgaggtcg
     1801 ggttggccac accaatcgcg taggacactt gcacctggca catgcgcgcc agacctgcgg
     1861 cgacgatgtt cttggccaca tagcggccag cgtaggcggc ggaacggtcc accttggacg
     1921 gatccttgcc ggagaaggcg ccgccgccgt gcggggctgc gccgccgtag gtgtcgacga
     1981 tgatcttgcg gccggtcaga ccgcaatcgc ccatcgggcc gccaatcaca aagcggccgg
     2041 tcgggttgat caggtacttg gtgtcggcgg tgagcatgtg cgccggcagc accggcttga
     2101 tgatgtcttc gatcaccgct tcggtcagcg tcttgtggtc gatttccggg gtgtgctggg
     2161 tagagagcac cacggtgtcg atggccttgg gcaagccggt ttcgctgtcg tacacgcagg
     2221 tgatctggct cttggcgtcc gggcgcagcc acggcagacg gccgtccttg cgcagttcgc
     2281 tctgacgctg caccagacgg tgggcgtagt aaatcgggaa cggcatcagc gtcggcgttt
     2341 cgtcgcaggc atagccaaac atcaggccct ggtcgccagc gccctggtcc agatccaggc
     2401 cttcgccttc gttcacgccc tgggcgatgt ccggcgattg cttgtcatag gccaccagca
     2461 cggcgcagcc cttggcgtca aagcccagat ccgagctgtc gtagccaatg cggcggatgg
     2521 tttcgcgggc gatgcccatg tagtcgatat tggcgtgggt ggtgatttca ccggccagca
     2581 ccaccagacc ggtgttgacc agggtttcgg ccgccacgcg ggcgtatttg tcttgagcca
     2641 ggatggcgtc caggatggcg tcagagattt ggtcggcgac tttgtccgga tggccttcgg
     2701 aaaccgattc cgaagtgaag aggtactcgc tcattgcgta atgtcctgaa gggtgattcc
     2761 gattcgggta aaatcttgcg tccgatttcc cgctggctcg tcatgtcaag attgctgatt
     2821 gctgtgtttt caatcctgtc gcatctgccc ctggccgtat tgcaggctgt gggcgttgcg
     2881 ctgggctggc tagtgtacct cagctcgccg cgctacgcgg cgcgcatgcg ggaaaacctg
     2941 ggccaaagcg gcctggcgac cgatgccgcc agccagcgcc gcttgctgcg ccaggcggtc
     3001 agcgccaccg gcatgggtgc gctggagttg gtgatcgcct ggggacgctc cccgacttct
     3061 atcgcccggt tggtgaaaaa ctgtaacaac tggcacattg tggaagaggc gctggcacaa
     3121 aacaagggca ttattttcat cacgccgcat ctgggcaatt acgatattgc cgggcgttat
     3181 ctgtcgcaac ggctgatcaa gcgcccgcaa gccattcagc tgatggcgat gtatcgcccg
     3241 ccgaaaatgg cctggctgga gcccttgatg aacgccggcc gcgtgcgtga aggcgggctg
     3301 actgcgccgg ccaccgccgc aggcgtgcgc atggtgatga aaaccctcaa ggcgcgccag
     3361 accaccatca tcctgcccga tcaggtcccc ggccagggag acggtgtgtg ggcgccattc
     3421 tttggccgcc cggcctacac catgaccctg gcgccgcgcc tggcgcaaat ggacggcgtg
     3481 gaggtaatcc tgttttttgg ccgccgcctg ggctggggac gcggctttga ggtggaaatc
     3541 gcccgcctgc ctgagccgtt caccggcgac aagaccacgg acggcgccaa actgaatcaa
     3601 gccgtcgagc agctgattcg ccaggcgcca gcgcagtatc tgtggagcta caaccgttac
     3661 aagacgcccg ccggcgtcgc ccctgccgag accccgaatc catgatgacc cgttttgccc
     3721 ttgctgtttt gtggttgttg cactggctgc cggtgccggt gctggccgtg ctggcccgcg
     3781 tactgggctg gctgggctac ctgctggcgc gcgagcgtcg gcgggttggc ctgatcaatt
     3841 tgcaacggtg ctttcctgac atgagcccgt cggcgcgccg gcgcattatc ctgcgtcact
     3901 gccagcacct gatgcagtcg ctgctggaat acggcattgc ctggtggggc agtccggcgc
     3961 ggctgcgccg gctggtgacg ctgaaaaacc tgcaccatct caccgatcag gctgagcaca
     4021 acaccatcat cctgtatcca cacttcatcg cctttgagct gtgtgtgttc cggctgaatc
     4081 tggagcaggc gctggtcagc gtgtattcca accagaaaaa cccggtgctg aataaacagt
     4141 tgtatcaggg gcgcagtcgc tttaataacg ccaagatcgt ctcgcgtcag gaagggctgc
     4201 gttccatcat caaggccatg cgcgaccata cgccattttt atacctgccc gaccaggact
     4261 ttggcccgcg cgactcggtg tttgtgaaat tctttgccga agacgccgcc accatcactg
     4321 ggctgtcgcg catctcggcg ctggccaagg ccaaagtggt gccggccatg gcgcggcggg
     4381 tgggctttcg ctacgagctg gagttttacc ctgcctggga aaacttcccg acagacgatg
     4441 taatggccga cacccgacgc atgaatgcat tcatcgagga gcgtgtgcgg gaagaccc
//
LOCUS       DAOPYX010000250         4488 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_24972_length_4488_cov_9.027295, whole genome shotgun sequence.
ACCESSION   DAOPYX010000250 DAOPYX010000000
VERSION     DAOPYX010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 4488)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4488)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4488
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24972_length_4488_cov_9.027295"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..403)
                     /locus_tag="PKN55_11430"
     CDS             complement(<1..403)
                     /locus_tag="PKN55_11430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX71899.1"
                     /translation="MTLNPSQRKWVLIAVALLIFAGVKFWLINTYLTRQHAAPQAISC
                     PDLQAGCTLPGGARVRFLQPARHGQRFGLELSQTPAGKAPPVAEFAMAGMDMGSARFP
                     LRQVGDGRWQADIMLPVCVTGRQDWLMLLTIG"
     gene            complement(400..1347)
                     /locus_tag="PKN55_11435"
     CDS             complement(400..1347)
                     /locus_tag="PKN55_11435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014086092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nudix family hydrolase"
                     /protein_id="HNX71900.1"
                     /translation="MTSTQLVEVVAGVLVRPDGRFLLASRPVGKVYAGYWEFPGGKIE
                     PGEAPYAALVRELQEELAITVTAATPWLVQQFVYPHAHVRLRFYRVSAWQGEPQAQEG
                     QQLNWQTPGQLDVAPMLPANTPILRALSLPAITALTCAGELGENAVLAALPARLAAGL
                     SLLIVREPDYARDQLASWVARLAELTRPAGCRLLVNAEPDWLAGWPVDGVHLNSARLA
                     ACRQRPDFAWVGASAHRPEELQHAAELGLDYALLGHVLPTASHPDAPPLGWQGLACQL
                     ASAGPRLPVYALGGLAAADLPAACEAGAHGVALMRGAWA"
     gene            complement(1362..2243)
                     /locus_tag="PKN55_11440"
     CDS             complement(1362..2243)
                     /locus_tag="PKN55_11440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014086088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNX71901.1"
                     /translation="MASSPDLAALLTRAESVLARLEAWLPPPPPATDWSAHAWRWRVG
                     AGGRGGLEAVHKPHGLALDALTGVDEQKTRLVRNTRQFIAGRPANNVLLTGARGTGKS
                     SLVKALLPAFAADGLRLIEVDKTHLADLADIVELLAERPERFIIYCDDLSFEEGDDAY
                     KALKTALDGGLARRADNVLIYATSNRRHLMPERMQDNLDSRNVNGEIHPGEAVEEKVS
                     LSERFGLWLSFYPFDQDTYLAAVAVWLAHHQLKLTPRAERAALLWALTRGARSGRVAW
                     QFACDWAGRTPAERKAD"
     gene            complement(2587..3261)
                     /locus_tag="PKN55_11445"
     CDS             complement(2587..3261)
                     /locus_tag="PKN55_11445"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009426169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HNX71902.1"
                     /translation="MNIALFDLDNTLLAGDSDVEWPRYLIGKGVLDADYYNRENDRFY
                     ADYKAGTLDIQAFLDFQLAPLAGRDRAELDAWHEEYLARHIRPIITARGRELIQQHQA
                     NGDLVVIITATNRFITGPIAAELGVEHLIATEAEIGADGRYTGKPTGTPCFQAGKITR
                     LNAWLAERGQSMASFADSWFYSDSRNDIPLLEQVNHPVAVDPDDALRAHAQDKGWPVV
                     SLRGAF"
     gene            complement(3498..4175)
                     /gene="hda"
                     /locus_tag="PKN55_11450"
     CDS             complement(3498..4175)
                     /gene="hda"
                     /locus_tag="PKN55_11450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011137161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0032297 - negative regulation of
                     DNA-templated DNA replication initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA regulatory inactivator Hda"
                     /protein_id="HNX71903.1"
                     /translation="MNQLTLDLSPPPAPAFDTFIAERNREVIAALTAAAPPAERFVHL
                     WGEGGSGKSHLLNAWVEHAHALGRAAIYLDARSERLPDFVREASAVAVDHVDDLDPDD
                     QITLFSVFNAFKESGGRLLTAGRLPPAQLALRPDLATRLASGLVLEVKPLSDNDKLAA
                     LRRHAKARQLNVPDEVFRYLLTHWRRDLSSLTAMLDTLDRYSLALQRPVTVPLVKNVL
                     QSRLLQD"
BASE COUNT          791 a         1447 c         1512 g          738 t
ORIGIN      
        1 cgccgatggt cagcagcatc agccagtcct ggcggccggt gacgcacacc ggcagcatga
       61 tgtcggcttg ccagcggccg tcgcccacct ggcgcagcgg aaaacgcgcg ctgcccatgt
      121 ccatgccggc catggcgaat tccgccaccg gcggcgcctt gccggcgggc gtctggctca
      181 gttccaggcc aaagcgctgg ccgtggcggg cgggctgcaa aaagcgcacg cgcgcgccgc
      241 cgggcagggt gcagccggct tgcaggtcgg ggcagctgat ggcttgcggg gcggcgtgct
      301 ggcgggtgag gtaggtgttg atcagccaga atttcacccc ggcaaaaatc agcagcgcca
      361 cggcaatcag cacccatttg cgttggctgg gattcagcgt catgcccacg ccccccgcat
      421 cagcgccacg ccgtgcgcgc cggcttcaca ggcggcgggc aggtcggcgg cggcaagccc
      481 ccccagcgcg tacaccggca gacgcgggcc ggcgctggcc agctggcagg ccaggccttg
      541 ccagcccagc ggcggcgcgt ccgggtggct ggcggtgggc agcacatggc ccagcagggc
      601 gtaatccagc cccagctcgg cagcgtgttg caattcttcc gggcgatgcg ccgaagcgcc
      661 cacccaggca aagtccgggc gctggcggca ggcggccagc cgcgcgctgt tcaggtgcac
      721 gccgtccacc ggccagccgg ccagccaatc cggctcggcg ttgaccagca gccggcagcc
      781 ggccgggcgg gtgagctcgg ccagccgcgc cacccagctg gccagttggt cgcgggcata
      841 atccggctcg cgcacaatca gcagcgacag cccggcggcc agccgcgccg gcagcgccgc
      901 cagcacggcg ttttcgccca gctcgcctgc gcaggtcagc gcggtgatgg ccggcagcga
      961 caaggcgcgc aggatggggg tgttggccgg cagcatcggc gccacgtcca gctggccggg
     1021 ggtttgccag ttcagctgct ggccttcttg cgcctgcggc tcgccctgcc aggcgctgac
     1081 ccgataaaag cgcagccgca catgggcgtg tgggtagaca aactgctgca ccagccaggg
     1141 cgtggcggcg gtgacggtga tggccagctc ttcttgcagc tcgcgcacca gcgcggcata
     1201 cggcgcttcg ccgggctcga tcttgccgcc ggggaattcc caatagccgg cgtacacctt
     1261 gcccaccggg cgcgaggcca gcaaaaaccg gccgtcgggg cgcaccagca ccccggcgac
     1321 cacttcaaca agttgggtgg aggtcatgtt ttgtgtgtcg tttaatccgc cttgcgctcg
     1381 gccggggtgc gcccggccca gtcgcaggca aactgccagg ccacccggcc gctgcgcgcg
     1441 ccacgggtga gcgcccacaa gagcgcggcg cgttcggcgc gcggggtgag tttcagctgg
     1501 tgatgcgcca gccacaccgc cacagcggcc agataggtgt cctggtcaaa tgggtagaac
     1561 gacagccaca ggccaaagcg ctcggacaac gagacttttt cttccacggc ttcgccgggg
     1621 tgaatttcgc cattgacgtt gcggctgtcc agattgtcct gcatgcgctc gggcatcagg
     1681 tggcggcggt tggaggtggc gtagatcagc acattgtcgg cgcggcgcgc cagaccgccg
     1741 tccagcgcgg ttttcagcgc cttgtaggcg tcgtcgcctt cttcaaacga caagtcatcg
     1801 caatagatga taaagcgctc gggccgctcg gccagcagtt cgacaatatc ggccaaatcg
     1861 gccagatggg ttttgtccac ttcgatcagc cgcaggccgt cggcggcaaa cgccggcagc
     1921 agcgccttga ccagcgagga tttgccggtg ccgcgcgcgc cggtcagcag cacattattg
     1981 gccgggcggc cggcgatgaa ctggcgggtg ttgcgcacca gccgggtttt ttgctcgtcc
     2041 accccggtca gcgcgtccag cgccaggccg tgcggcttgt gcaccgcttc cagcccgccg
     2101 cgcccgccag cgcccactcg ccagcgccag gcgtgcgcgc tccagtcggt ggcgggcggc
     2161 ggcggcggca gccaggcttc cagccgggcc agcaccgatt cggcgcgggt gagcaaggcg
     2221 gcaagatcag gggacgaagc catccgggct atcctttcat ttgggcggag cattggctac
     2281 agccaaaccc cgcaaaatca acaccctgga ttcccgccag cgcgggaatg acgatttttt
     2341 cagcgtaggc tgagcggcgc ttggcgcggc tcacaacacc caccgcagcg cgcctgacta
     2401 ggcgacgacc gcagacagca taagaaacgg caaggccggg caacaacgct aggcgggttt
     2461 tgtgagcggc gctaagagcc gctaacaaaa ctcagcgaag cggtcctggc taggcgtgtg
     2521 accgcagaca gtacaagcag tacggcaagg tcacacaaca acgccagggg gttttgttag
     2581 cggcgcttaa aacgccccgc gcagcgacac caccggccaa cccttatcct gtgcatgggc
     2641 gcgcagcgcg tcgtccgggt ctaccgccac cgggtgattc acctgctcca gcagcggaat
     2701 atcattgcgc gaatcactgt aaaaccagct gtcggcaaag ctggccatgc tctggccgcg
     2761 ctcggccagc caggcgttca gccgggtgat cttgcccgcc tgaaaacacg gcgtgccggt
     2821 gggtttgccg gtgtagcggc catcggcgcc gatttcggct tcggtggcga tcaggtgttc
     2881 cacgcccagt tcggcggcaa tcgggccggt gataaagcgg ttggtggcgg tgatgatcac
     2941 caccaggtcg ccattggcct gatgctgctg gatcagctcg cgcccgcgcg cggtgatgat
     3001 cgggcggatg tggcgcgcca ggtattcctc atgccaggcg tccagctcgg cgcggtcgcg
     3061 cccggccagc ggggccagct ggaaatccag aaacgcctga atatccagcg tgccggcctt
     3121 gtagtcggcg taaaagcggt cgttttcgcg gttgtagtag tcggcgtcca gcaccccctt
     3181 gccaatcagg taacgcggcc attccacgtc ggaatcgcca gccagcaggg tgttgtcgag
     3241 gtcaaacagg gcaatattca tcgggcttca caacagaaga aaagtaaaga cggcaggcct
     3301 taagagccgc taacaaaacc cgcctggcgt tgttgtgtga ccttgccgta ctacctgtac
     3361 tgtctacggt cacacgccta gccaggcgcg cttcgctggg ttttgttagc gactctaaga
     3421 gccgctaaca aaacctgcct ggcgttgctt gcgctgtctg ctgccaagcg cgctgcgctg
     3481 ggtttgctga gcggcgctta gtcttgcagc agccgcgatt gcagcacatt tttcaccagc
     3541 ggcaccgtca ccggccgttg cagcgccagc gaatagcggt ccagggtgtc gagcatggcg
     3601 gtgaggctgg aaagatcgcg ccgccagtgg gtcagcaaat agcgaaacac ctcatccggc
     3661 acattcagct ggcgcgcctt ggcgtggcgg cgcagcgcgg ccagtttgtc gttatccgac
     3721 aaaggcttga cctccagcac caggccagac gccagccggg tggccaagtc cgggcgcaag
     3781 gccagctgcg ctggcggcaa gcgcccggcg gtcagcagcc gcccgccgga ttctttaaag
     3841 gcgttgaaca ccgaaaacag ggtgatctgg tcgtccgggt cgaggtcatc gacgtgatcc
     3901 accgccaccg cgctggcctc acggacaaaa tccggcagcc gttcgctgcg cgcatccaga
     3961 taaatcgccg cccgccccag cgcgtgggcg tgctccaccc aggcgttaag caggtgcgat
     4021 ttgccgctgc cgccttcgcc ccacaggtgc acaaagcgtt cggcaggcgg cgcggcggcg
     4081 gtcagcgcgg cgatgacttc ccggttgcgc tcggcaataa aggtgtcaaa ggccggcgcg
     4141 ggcggcgggc tgaggtcgag ggtcagttgg ttcaacgtga tgcgccggcg cggccggccc
     4201 gaatgcggtc gaaaagcaga ttttaccgca tttgcgtgaa aagctcgtgg gcttgtgggt
     4261 aattggtgtc tggccgtggc cagcgaccca gattggggga tgtcagtgca agattgcgcc
     4321 cggacgacag gcaaaaaaat actggcaaga gaacggtcat ttacaacctg aatcaggtta
     4381 ttgatgccat tttcttgcca gcttgtgttg ccaaaccatg gatttgacaa tgaatattgg
     4441 tggaggcggc ggggatcgaa cccgcgtccg gaaatcctct acggtgag
//
LOCUS       DAOPYX010000251         4429 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_25442_length_4429_cov_11.933013, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000251 DAOPYX010000000
VERSION     DAOPYX010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 4429)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4429)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4429
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25442_length_4429_cov_11.933013"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            58..981
                     /gene="pqqB"
                     /locus_tag="PKN55_11455"
     CDS             58..981
                     /gene="pqqB"
                     /locus_tag="PKN55_11455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014088312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone biosynthesis protein
                     PqqB"
                     /protein_id="HNX71904.1"
                     /translation="MLRAIVLGAAAGGGLPQWNCACPVCAAVRSGQAGAPQTQSSIVV
                     SADGERWVLINASPDIRQQFAATPALHPRAVRHSPLSAVLVTNADVDHVAGLLSLREA
                     QPFALYATRRVHSVLDANAIFNVVNREHVPRRPVQLNESMPILDAAGEPTGICIETFT
                     VPGKVALWLEDPNAERFGSVAEDTIGVAIRAEGSEARLFYLPGCADVPDSLKARFKPT
                     DTVLFDGTTYTEHEMAEAGVGQKSASRMGHLVMSGPQGTIARLADVPLARRLFIHINN
                     TNPVWLPDSEARREIARQGWDLAFDGMELTL"
     gene            981..1727
                     /gene="pqqC"
                     /locus_tag="PKN55_11460"
     CDS             981..1727
                     /gene="pqqC"
                     /locus_tag="PKN55_11460"
                     /EC_number="1.3.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008952531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline-quinone synthase PqqC"
                     /protein_id="HNX71905.1"
                     /translation="MTRLTRLLSPDELEAALRAIGRERYHNLHPFHHLLHGGKLSLGQ
                     VQAWALNRYCYQAAIPRKDAALISKIHDRELRRVWAQRIIDHDGREQEEGGIERWLVL
                     TDGLGLPRDYVISREGALPATVFAVEAYVRFVAEQPPVVAVSSCLTELFAPAIHKERI
                     VGMLENYDFIEDRVLAYFRRRLDQAPRDADFALDYVKRHALTIAEQQGVIDALLFKCN
                     VLWAQLDALYAAYVAPGMVPPGAFRPEDWA"
     gene            1724..1996
                     /gene="pqqD"
                     /locus_tag="PKN55_11465"
     CDS             1724..1996
                     /gene="pqqD"
                     /locus_tag="PKN55_11465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008952530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone biosynthesis peptide
                     chaperone PqqD"
                     /protein_id="HNX71906.1"
                     /translation="MIAADSVVRLAPGVRMQQDAARGGWVLLGPERMLVLDDIAQLVI
                     SRLAGQSAGELVAALAVEFDAPVEVIGADVLALLNTLADKGFVRYG"
     gene            1989..3086
                     /gene="pqqE"
                     /locus_tag="PKN55_11470"
     CDS             1989..3086
                     /gene="pqqE"
                     /locus_tag="PKN55_11470"
                     /inference="COORDINATES: protein motif:HMM:TIGR02109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018149 - peptide cross-linking [Evidence
                     IEA];
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone biosynthesis protein
                     PqqE"
                     /protein_id="HNX71907.1"
                     /translation="MASLLPNTLPLAMLMELTHRCPLRCAYCSNPLALSPASAELPTA
                     AWKAALDEAAQLGMLQVHFSGGEPMARPDLIELVRHATARGLYSNLITSGLLLDAHKL
                     AELVDAGLSHVQLSFQDLDEDGAQWVSGYAGGVAKKREVADRVLASGLALTLNFVMTR
                     HNVSRLSAMLDYAESLGVGRVEVANTQYYGWGLKNRAALIPTREQLDEMNSLVAERRA
                     RCKGRMVIDYVIPDYYARRPKACMGGWGQRFINVMPDGTILPCHAAQTIGHLSFPRFP
                     ESSLRAAWCEHPSFAAYRGVDWMPDPCGSCDHKEQDWGGCRCQALALAGDASQTDPVC
                     ERSPQHAQVVALAMRESRQPTPELMLRQRHA"
     gene            complement(3217..4353)
                     /locus_tag="PKN55_11475"
     CDS             complement(3217..4353)
                     /locus_tag="PKN55_11475"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNX71908.1"
                     /translation="MFGRQKIADLQARLSQIESERSQLQRELQAAQTALAEQQQTSHG
                     LQGELEQNRTLRNPFAEFCQSIVDIRTSFSTLAERMDSCFVSAADAATTLHGTRGMVD
                     VLTDSFTQIAASQQQTRQHIDTLDGKTGEIRQFVQLIKEVADQTNLLALNAAIEAARA
                     GESGRGFAVVADEVRKLAERTAAATREIDVLVEDIGEASGATKQQVSEASEQILHYRD
                     VGGRATASIHRLVDDTQDMAQAIAYGANVSFMEVVKLDHIVFKLEIYKAVMGYHTLQP
                     DSIASHQTCRLGKWYYQGRGSQECADLPAFRQLETPHQQVHDYARHALLALEIGDRKE
                     AELSLLRMERASVKVMELLTEMESIPCAYTLEGMALGQGAKAAA"
BASE COUNT          707 a         1466 c         1448 g          808 t
ORIGIN      
        1 atcgccctcg gcctggaaat caactgctac atgtgcgctg agctgtaagg cgcgatcatg
       61 ctgcgcgcca ttgttttagg cgccgccgcc ggcggcggtc tgccgcagtg gaactgcgcc
      121 tgcccggtat gcgcggcggt gcgcagtggg caggccggcg cgccgcagac ccaatcttcg
      181 attgtggtca gcgccgacgg cgagcgctgg gtgttgatca acgcctcccc tgacattcgc
      241 caacaatttg ccgccacccc ggcgctgcat ccgcgcgccg tgcggcacag cccgctgtcg
      301 gcggtgctgg tgaccaacgc cgacgtcgat cacgttgccg gcctgttgtc gctgcgcgaa
      361 gcccagccgt ttgccctgta cgccacccgc cgggtgcaca gcgtgctgga cgccaacgcc
      421 atcttcaacg tggtcaaccg cgaacatgtg ccgcgtcgcc cggtgcaact gaacgaaagc
      481 atgccgattc tggacgccgc tggcgaacct accggcatct gcatcgaaac cttcaccgtg
      541 cccggcaaag tggcgctgtg gctggaagac ccgaatgccg agcgctttgg cagcgtggcc
      601 gaagacacca tcggcgtggc catccgcgcc gaaggcagcg aggcccggct gttttacctg
      661 cccggctgcg ccgacgtgcc cgacagcctg aaagcgcgct tcaagcccac cgacaccgtg
      721 ctgtttgacg gcaccaccta caccgaacac gaaatggccg aagccggcgt cggccagaaa
      781 tccgcctcgc gcatgggcca tctggtgatg agcggcccac aaggcaccat tgcccggctg
      841 gccgatgtgc cgctggcgcg ccgtttgttc attcatatca acaacaccaa cccggtgtgg
      901 ctgcccgaca gcgaggcgcg ccgggagatt gcccgccagg gttgggatct cgccttcgat
      961 ggcatggagc tgacattgtg atgacccgcc tgacccgcct gctgtcgccc gacgagctgg
     1021 aagccgccct gcgcgccatc ggccgcgagc gctaccacaa cctgcacccg tttcatcacc
     1081 tcttgcacgg cggcaagctg tcgctgggtc aggtgcaggc ctgggcgctg aaccgctact
     1141 gttatcaggc ggcgattccg cgcaaggacg ccgcgctgat cagcaagatt cacgaccgcg
     1201 aactgcgccg ggtgtgggcg cagcgcatca tcgaccacga tggccgcgaa caagaagaag
     1261 gcggcatcga gcgctggctg gtgctgaccg acggcctggg cctgccgcgt gactatgtga
     1321 tttcccgcga gggcgcgctg ccggccacgg tgtttgccgt ggaagcctat gtgcgctttg
     1381 tcgccgaaca gccgccagtg gtggcggtgt cgtcctgcct gaccgagctg tttgccccgg
     1441 cgattcacaa agagcgcatc gtcggcatgc tggaaaacta cgactttatc gaagaccgcg
     1501 tgctggccta cttccgccgc cggctggacc aagcgccgcg cgatgccgat tttgccctgg
     1561 actacgtcaa gcgccacgcg ctgaccatcg ccgagcaaca aggcgtgatc gacgcgctgc
     1621 tgttcaagtg caatgtgctg tgggcgcagc tggacgcgct gtacgccgcc tatgtcgccc
     1681 ccggcatggt tccgcctggc gccttccgcc ccgaggattg ggcatgattg ccgccgacag
     1741 cgtggtgcgc ctggcccccg gcgtgcgcat gcagcaagat gccgcgcgcg gcggctgggt
     1801 gctgctgggg cccgagcgca tgctggtgct ggacgacatc gcccaactgg tgatttcgcg
     1861 cctggccggc caaagcgccg gcgagctggt cgccgcgctg gcggtggagt ttgacgcgcc
     1921 ggtcgaggtg attggcgccg atgtgctggc gctgctcaac accctggcag ataaaggatt
     1981 tgtccgctat ggctagcctg ttgcccaaca ccctgccgct ggccatgctg atggagctca
     2041 cccatcgctg cccgctgcgc tgcgcctact gctccaaccc gctggcgctc agcccagcca
     2101 gcgccgagct gcccaccgcc gcctggaaag ccgcgctgga cgaggccgcc cagctgggca
     2161 tgctgcaagt gcatttttct ggcggcgagc cgatggcgcg cccggacttg atcgagctgg
     2221 tgcgccacgc caccgcgcgc gggctgtaca gcaatttgat cacctccggc ctgctgctgg
     2281 acgcgcacaa actggccgaa ctggtggacg ccgggctgtc gcatgtgcag ctgagttttc
     2341 aggatctgga cgaagacggc gcgcagtggg tgagcggcta tgctggcggg gtggcgaaaa
     2401 aacgcgaagt ggccgaccgc gtgctggcca gcggcctggc gctgacgctg aattttgtca
     2461 tgacccggca caatgtctcg cggctgtcgg ccatgctgga ttacgccgaa agcctgggcg
     2521 tgggccgggt ggaagtggcc aacacccaat actacggctg gggcctgaaa aaccgcgcgg
     2581 cgctgattcc cacccgcgaa cagctggacg agatgaacag cctggtggcc gagcggcgcg
     2641 cgcgctgcaa gggccgcatg gtgattgatt atgtgatccc ggattattac gcccgccggc
     2701 ccaaggcctg tatgggcggc tggggacagc gctttatcaa tgtgatgccg gacgggacca
     2761 tcctgccctg tcacgccgcg caaaccatcg gccacctgag ttttccgcgc tttcctgaaa
     2821 gcagcctgcg cgccgcctgg tgcgagcacc ccagctttgc cgcctatcgc ggcgtggact
     2881 ggatgcccga cccgtgcggc agctgcgacc acaaagaaca ggattggggc ggctgtcgct
     2941 gccaggcgct ggccctggcc ggcgacgcca gccaaaccga cccggtgtgc gaacgctcgc
     3001 cgcagcacgc acaggtggtg gcgctggcca tgcgcgaatc gcgccagccc acgcccgagt
     3061 taatgctgcg ccagcgccac gcttagcgtg gcggcacaac cgtcatcacg gtttctccct
     3121 gctccctggg tatttgcggg cgccgcctgg gccccgcgcc acgtgtgtct cgtgggtgtt
     3181 tggccagccg gcgtgtccgg ctggtttttt tctggctcag gccgcggctt tagcgccttg
     3241 acccagcgcc atgccttcca gcgtatacgc gcagggaatg ctttccatct cggtcaataa
     3301 ttccatcacc ttcaccgacg cgcgctccat gcgcagcagc gacagctcgg cctctttgcg
     3361 gtcgccaatc tccagcgcca gcagcgcatg gcgggcatag tcgtgcacct gctgatgcgg
     3421 ggtttccagt tggcggaacg ccggcaggtc ggcgcattcc tggctgccgc gcccctggta
     3481 gtaccacttg cccagccgac aagtctggtg cgaggcgatg ctgtccggct ggagcgtgtg
     3541 gtagcccatc accgccttgt agatttccag cttgaagaca atgtgatcca gcttgaccac
     3601 ttccataaag ctgacattgg cgccgtaggc aatcgcctgc gccatgtcct gggtgtcgtc
     3661 caccagccgg tgaatgctgg cggtggcgcg gccacccaca tcgcggtagt gcagaatctg
     3721 ttcgctggcc tcgctcacct gctgcttggt ggcgcccgag gcttcgccga tgtcttccac
     3781 cagcacgtca atttcgcggg tggcggcggc ggtgcgctcg gccagcttgc gcacttcgtc
     3841 ggccaccacg gcaaaaccac ggccggattc accagcgcgg gcggcttcga tggcggcgtt
     3901 cagcgccaag agattggtct ggtcggccac ttccttgatc agttggacaa actggcggat
     3961 ttcgccggtt ttgccatcca gggtgtcgat gtgctggcgg gtttgctgct ggctggcggc
     4021 aatctgggta aagctgtcgg tcagcacgtc caccatgccg cgcgtgccgt gcagcgtggt
     4081 ggcggcgtcg gcggcgctga caaagcagct gtccatgcgc tcggccaggg tggaaaacga
     4141 ggtgcggata tcgacaatgc tctggcaaaa ctcggcaaac ggattacgca aagtgcggtt
     4201 ctgctccagc tcgccttgca ggccgtggct ggtttgctgc tgctcggcca aggcggtttg
     4261 cgcggcctgc aactcgcgct gcaactggct gcgttcgctt tcgatctggc tcagccgggc
     4321 ttgcagatcg gcgatttttt gacgtccaaa catgacaact ccactgcgct ggccagtcat
     4381 ctgaccaggg tgcgcgcagg ggcggcgatg tcaaatccgg ccggcgcgc
//
LOCUS       DAOPYX010000252         4392 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_25751_length_4392_cov_8.355776, whole genome shotgun sequence.
ACCESSION   DAOPYX010000252 DAOPYX010000000
VERSION     DAOPYX010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 4392)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4392)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4392
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25751_length_4392_cov_8.355776"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..234
                     /locus_tag="PKN55_11480"
     CDS             <1..234
                     /locus_tag="PKN55_11480"
                     /inference="COORDINATES: protein motif:HMM:NF014696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HNX71909.1"
                     /translation="SPVGLVAVLPVGMCQVSSVVMTADVGVKLRKGEEFAYFQFGGSD
                     VVMLFESSVNAGLIMQPGVHYKQGSWIGHAFPG"
     gene            468..2462
                     /locus_tag="PKN55_11485"
     CDS             468..2462
                     /locus_tag="PKN55_11485"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNX71910.1"
                     /translation="MTLFTHSIRHRITFLAVCIAACIFALSMLVSTLDSRDNALKAAQ
                     DNARLLAEREAARLEGELKRTFTSARTLAQALQGMKAAGTPPSREQIDHAMREMLQQT
                     PDVLSYGSLWEPNALDGRDSEYAGRAPSHDKTGRYLPYWNRASGQIAVEPLVDYDKPG
                     ANDWYVVPQKTLQDIFTDPYPYKVAGREVWVATVSTPVLVSGKFAGIVALDYPLEGLQ
                     KSLANVRPFASGYAALVSNGGFYASHPNDKLLGKKADTLSPQALEAIAQGKPYQFEAA
                     GQMYVFAPLHLGNGAAPWSMMVSFPMSAVLADANQLVIKMAIIGLIGVAVMTVVLALG
                     IRKMIRPLEKLTLTVSELEGDLTVRLPVSRKDEIGQIAQAFNVFMGRLQPVVRDIRTH
                     SESVDQTGQALAELTQTIADRSHQQSQASQSTASAMEEIATGISHVANSAHQAGVEAE
                     ATEDFIDDATASIQGMAKDIRAVNATIGEVKTRVSQLDVNAREIDRIAQAIQDIAEQT
                     NLLALNAAIEAARAGEQGRGFAVVADEVRKLAERTTQATLEIGKLLQTIQKETGLVVD
                     VVEHAASSTDAGATRSDETVKLIDMIHQRVQSMAAQTSAIAQASAQQAQACNEVAMHV
                     ETISAAAQENDQAVSEAEVSVRSMCQKVTQLGDKVRMFKV"
     gene            2791..>4392
                     /gene="fdnG"
                     /locus_tag="PKN55_11490"
     CDS             2791..>4392
                     /gene="fdnG"
                     /locus_tag="PKN55_11490"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011137386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HNX71911.1"
                     /translation="MDHHHHAAAGVSVSRRQFFKISAAGMSATSLAVMGMAPATAQAE
                     VRQYKLSRATEVRNTCTYCSVGCGLLMYSLGDGAKNAKQEIIHIEGDPDHPVSRGSLC
                     PKGAGLLDFVHSPGRLKYPEVREAGSDQWKRISWHEAVERIAKHMKADRDANFIDKNA
                     DGVPVNRWLSTGMLTASASSNETGILTQKFMRSLGIIATDAQARVCHGPTVAALASTF
                     GRGAMTNSWVDIKNADFILVMGGNAAEAHPVGFKWAIEAKKQRGAKLIVVDPRFNRTA
                     AVADQYVPIRAGSDIVFLGGIINWLIANDKIHWDYVKAYTNASLIVKEEYGFDEGLFS
                     GFDAEKSKYDRASWNYELDANGAAKRDPSLEHPRCVWNLMKAHFARYTPELVSSLTGS
                     PKEGFLAVCQHLGETAAPNKVGTILYALGWTQHTVGAQNIRTMAMIQLLLGNIGMPGG
                     GVNALRGHSNIQGLSDLGLLSTSLPGYLTLPNETQHPTLADYLAKNTPKPLQDGQFNY
                     WSNTPKFFVSLMKWFWGDKATKDNNW"
BASE COUNT          944 a         1420 c         1266 g          762 t
ORIGIN      
        1 agcccggtgg gcttggtcgc agtactgcca gtgggcatgt gtcaggtgtc gtcggtggtc
       61 atgaccgccg acgtgggcgt caagctgcgc aagggcgaag aatttgccta tttccagttt
      121 ggcggctcgg atgtggtgat gctgtttgaa tccagcgtca atgccggcct gatcatgcag
      181 ccgggcgtac actacaagca gggcagctgg atcggtcacg ctttcccagg ctgatgacat
      241 gaaaaactgg caaaatacca tgccatatgc aataatgcgc gctttgaaat ttgctgcgcg
      301 ccgcacttga agttttgccc ttatgggatg ccataaaaca tcacgggtgg gagtggcgcg
      361 caatcatcat gacagagtcg ctgtcgcgtt cttacgacag tgcctgtcat gttttgccag
      421 ccacgtgcac agaaaaatat cccgaatgtc cttgaacggt tcttgccatg accctcttta
      481 ctcactcgat acgccaccgt atcacttttc tggcggtgtg cattgccgcc tgcatctttg
      541 cgttgtccat gctggtcagc acgctggaca gccgcgacaa tgcgctgaag gcggcgcagg
      601 ataacgcccg cctgctggcc gagcgcgagg ccgcgcgact ggaaggcgaa ctcaagcgca
      661 cctttaccag cgcacgcacc ctggcacagg cgctgcaagg catgaaagcc gctggcacgc
      721 cgcccagccg cgaacagatc gaccacgcca tgcgtgaaat gctccagcag acaccggatg
      781 tgctgtctta cggctcgctg tgggaaccca acgccctgga cggtcgcgac agcgaatatg
      841 cgggccgtgc ccccagccac gacaaaaccg gccgctacct gccgtactgg aaccgggcct
      901 ccggccagat tgccgtagaa ccgctggtgg actacgacaa acccggagcc aatgactggt
      961 acgtggtgcc gcaaaaaacc ctgcaggaca tttttaccga cccttacccc tacaaggtag
     1021 ccggccgcga agtatgggtg gccacggttt ccaccccggt gctggtcagc ggcaagtttg
     1081 ccggtattgt tgcgctggat tacccgctgg aaggcctgca aaaatcgctg gccaatgtcc
     1141 gcccgtttgc ttccggctat gcagccctgg tgtccaatgg cggtttttac gccagccacc
     1201 ccaacgacaa gctgctgggc aaaaaggccg acaccctgtc gccgcaggca ctggaagcga
     1261 ttgcccaggg caaaccctat cagtttgagg ctgccggtca gatgtatgta tttgccccgc
     1321 tccacctggg caacggcgcc gccccctggt cgatgatggt cagttttccg atgtcggcgg
     1381 tgctggccga cgccaatcaa ctggtgatca agatggccat cattggcctg attggcgtag
     1441 cggtgatgac ggtggtgctg gcgctgggca tccgcaagat gattcgcccg ctggaaaaac
     1501 tcaccctcac cgtcagcgag ctggaaggtg acctcaccgt acgcctgcca gtcagtcgca
     1561 aggacgaaat cggccagatc gcccaggcgt tcaatgtctt catggggcgc ttgcagcccg
     1621 tggtgcgcga tattcgcacc cacagcgaat cggtggatca aaccggccag gcgctggctg
     1681 agctgacgca aaccattgcc gaccgctcgc atcagcaaag ccaggcctcg caatccaccg
     1741 ccagcgccat ggaagaaatc gccaccggca tcagccatgt ggccaactcc gcccatcagg
     1801 ccggcgtgga ggccgaagcc accgaagact ttattgacga cgccaccgcc agcattcagg
     1861 gcatggccaa ggacatccgc gcggtgaatg ccaccatcgg cgaagtcaaa acccgtgtgt
     1921 cacaactgga tgtcaacgcc cgcgaaatcg accgcatcgc ccaggccatt caggatattg
     1981 ccgagcaaac caacctcctg gccttgaacg ccgccatcga agccgcccgc gccggcgaac
     2041 agggccgcgg ctttgccgtg gtggcggatg aggtgcgcaa gctggctgaa cgcaccacgc
     2101 aggccacgct ggaaatcggc aagctgctgc aaaccatcca gaaagaaacc ggcctggtgg
     2161 tcgatgtggt tgaacacgcc gccagcagca ccgatgccgg cgccacccgt tcggatgaaa
     2221 ccgtcaagct gatcgacatg attcaccagc gggtgcaaag catggcggcg caaaccagcg
     2281 ccatcgccca ggccagcgcc cagcaggcac aggcctgcaa tgaagtggcc atgcacgtgg
     2341 aaaccatcag cgccgccgcg caggagaatg atcaggcggt gtctgaggca gaagtctcgg
     2401 tgcgcagcat gtgccagaaa gtcacccagt tgggcgacaa agtgcgtatg ttcaaggtat
     2461 aaacgcacac catctgacgg gatgcccatc agtacacggg gcggacattg tccgccccgt
     2521 atcattttgc cctcctgcct ttgcccgcca ggctaggtca ttttgtccag cccgccgcca
     2581 aaatgacccc ccattctgac tgcccccctt ggcgttacac gccaaaccca tgatcttcat
     2641 ggggtttctt ccatggcaca gctcttgcct atggcccagc cgctttggag ggacgccccc
     2701 cacgccgaat cttggccaac acgtccctcc cgatcccccg caacggccgc ctcgcatgcg
     2761 cgccgttgag ctaaaaccag gaggagatat atggatcacc atcaccatgc cgctgctggc
     2821 gtctcagtca gccgacggca gtttttcaag atttccgccg ccggcatgag cgccaccagc
     2881 ctggcggtca tgggcatggc cccggccacc gcgcaggcgg aagtgcgcca gtacaaactc
     2941 agccgcgcca ccgaagtgcg caacacctgc acctattgct cggtgggctg tggcttgctg
     3001 atgtacagcc tgggcgacgg cgccaagaac gccaagcagg aaatcatcca catcgaaggc
     3061 gatccggatc acccggtcag ccgtggctcg ctgtgcccca aaggtgccgg cctgctggac
     3121 tttgtacaca gcccgggccg actgaaatac ccggaagtgc gcgaagccgg cagcgaccaa
     3181 tggaagcgca tcagctggca cgaagccgtc gagcgcatcg ccaagcacat gaaggccgac
     3241 cgcgacgcga atttcatcga caagaacgcc gacggcgtgc cagtcaaccg ctggttgtcc
     3301 accggcatgc tgacggcctc ggcctcgtcc aacgaaaccg gcatcctcac gcaaaaattc
     3361 atgcgctcgc tgggcattat cgccaccgat gcgcaggcgc gtgtctgtca cggcccgacc
     3421 gtggcggcac tggcctccac ctttggtcgt ggcgccatga ccaacagctg ggtggacatc
     3481 aagaacgccg acttcattct ggtgatgggc ggcaacgccg ccgaggcgca cccggtgggc
     3541 ttcaagtggg cgatcgaggc aaaaaaacaa cgcggcgcca agctgatcgt ggtcgatcca
     3601 cgctttaacc gcaccgccgc cgtggccgac caatacgtgc cgatccgcgc cggctcggac
     3661 attgtgtttc tgggtggcat catcaactgg ctgattgcca acgacaaaat tcactgggat
     3721 tacgtcaagg cgtataccaa cgccagcctg atcgtcaaag aagaatacgg attcgacgag
     3781 ggcttgttct cgggctttga cgcggagaaa agcaaatacg accgcgccag ctggaactac
     3841 gagctggacg ccaacggcgc cgccaagcgt gaccccagcc tggaacatcc acgctgcgtg
     3901 tggaacctga tgaaggccca cttcgcccgc tacaccccgg aattggtcag cagcctgact
     3961 ggcagcccga aagaaggctt tctggcggtg tgtcagcacc tgggcgaaac cgccgcaccg
     4021 aacaaagtcg gcactattct gtacgcgctg ggctggacgc aacacacggt gggcgcacaa
     4081 aacatccgca ccatggcgat gatccagctc ctgctgggca atatcggcat gcccggcggc
     4141 ggcgtcaacg cactgcgcgg ccactccaac atccagggct tgtccgacct gggcttgctg
     4201 tccacctcgc tgccgggcta cctgaccctg cccaacgaaa cccagcaccc gaccctggcc
     4261 gactacctgg ccaagaacac gcccaaaccc ctgcaggatg gccagttcaa ctactggagc
     4321 aacacaccca agttctttgt cagcctgatg aagtggttct ggggcgacaa ggccaccaag
     4381 gacaacaact gg
//
LOCUS       DAOPYX010000253         4343 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_26192_length_4343_cov_9.733909, whole genome shotgun sequence.
ACCESSION   DAOPYX010000253 DAOPYX010000000
VERSION     DAOPYX010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 4343)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4343)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4343
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26192_length_4343_cov_9.733909"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            87..980
                     /locus_tag="PKN55_11495"
     CDS             87..980
                     /locus_tag="PKN55_11495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011135456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HNX71912.1"
                     /translation="MSFRDIAAALLVVWVWGVNFVVIKWGVHDLPPLLLGGLRFVLAA
                     LPAGWLIARPALPWPYVLAYGLSMGVGQFGLLFSAMAYGMPAGLASVVLQAQAFFTLL
                     LGGLWLGERIALASLSGLLCASVGLWLIASTHGANMTMLGFALTLAAALSWALSNLVV
                     RRASQAGHRPDTLALVVWASLVPPLPLLAASLLFEGPARIGAALADLSWRGGLAVLYL
                     AFIATLVGYSVWSRLLTRYPAGKVAPFSLLVPAVGLCSASWLLDERLTMQQLAGSACV
                     LTGLLIHLSGGSLFARLRRPA"
     gene            1015..1986
                     /locus_tag="PKN55_11500"
     CDS             1015..1986
                     /locus_tag="PKN55_11500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012697196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNX71913.1"
                     /translation="MAQWPTPPEIEIYCVLQPRFLLLDFAGAAEAFRLANDYGGRFRL
                     RYVGPDAAPANSLGLPIHTIDPLPDSLPEGAWVMLSGVTESAKDYTLPATKTTRDWLK
                     RTFRPNQHTLVCVCSGALLAGMAGLLDGRRCTTHHTLTARLAQQSPKADVLENRIFVE
                     DGPIHTSAGVTAGVDLALHLIARHAGPAVALAVAREMVVYLRREGNDAQLSPWLANRN
                     HLHPAVHRAQDLIAQDPQRHWPVSELAERVHVSPRHLARLFAQHAGLSIIDYQHRLRV
                     ALARERLERGESVECAAQQAGFGSARSLRRVWQKFEHLSPGAVRTRA"
     gene            complement(2098..3378)
                     /locus_tag="PKN55_11505"
     CDS             complement(2098..3378)
                     /locus_tag="PKN55_11505"
                     /inference="COORDINATES: protein motif:HMM:TIGR02765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003913 - DNA photolyase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DASH family cryptochrome"
                     /protein_id="HNX71914.1"
                     /translation="MSVMIYWFRQDARLHDAPALRWAAERASALCPVVCLPPESTTRW
                     GVPRLGPHRQAARGQTWLGLDASLRALGSRLAVLHGEPAQALPALARQLGADTVVCER
                     IAAPEEDAEVAALRAAGLTVREHWQSGLFEPSALPFAVADLPKVFSQFRQQLSRAGCQ
                     PLAPLPALSQLPPWPAAAPVLPGPSEAPCPPLASGCAFPYALPAWRLDEAAGLAHLAG
                     YCAGEDIARYKATRNGLSGTAYSSKFSPWLACGALSVRQVWAALAARDAEYGVSEGTE
                     WLRFELLWREYFRLLHLACGRQLYGVSGLNPQAPAPRHHPASYARWCEGRTGQPLVDA
                     AMRELAASGYLSNRLRQVAASYLLHELDDDWRAGAAWFEAQLVDYDVYSNQGNWLYIA
                     GRGTDPRGGRRFNPDKQAAEYDADGAYRRLWGTA"
     gene            complement(3466..>4343)
                     /locus_tag="PKN55_11510"
     CDS             complement(3466..>4343)
                     /locus_tag="PKN55_11510"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNX71915.1"
                     /translation="KQGGCMSWFAWALVLAGAVCHALWNLIAKQVASGGAPFVWLYGV
                     VSLCWLTPLAIWQAQALTWTPAMLAVVLGSALLHAVYSLVLQRGYQRAPFSLVYPLAR
                     GSGPLLSALGATLWLAEAPSVRGWGGIALLIAGIGLLSRGGQAEADPARQRAGIGWGL
                     ATGACIAAYTLVDGVAVKTLAMPPLVFYGYSLLARSVLLAPLAWRQPQAMRRQARDHW
                     RAALLVGVLSPLAYALVLFALQIAPLSYVAPVREVSMLLASWLGARYLGEALTAQRLA
                     GSALMLLGVALLAGA"
BASE COUNT          696 a         1536 c         1427 g          684 t
ORIGIN      
        1 tcttagcgcc gctttgattg ttgtggcgca tcacgcccta gcagccgccc catccgagtg
       61 cgctgccata cagaacagga ccatttatgt catttaggga tattgccgcc gccctgttgg
      121 tggtgtgggt gtggggggtg aactttgtcg tcatcaagtg gggcgtgcac gacctgccgc
      181 cgctgttgct gggcggcctg cgctttgtgc tggcggcgct gccagcgggc tggctgattg
      241 cccgcccggc gctgccatgg ccttatgtcc tggcctacgg gctgagcatg ggcgtgggtc
      301 agtttggctt gctgttttcc gccatggcct acggcatgcc ggccgggctg gcctcggtgg
      361 tgttgcaggc gcaggcgttt ttcaccctgc tgctgggcgg gctgtggctg ggcgagcgca
      421 ttgccctggc gtctttgtcg ggtttgttgt gcgccagtgt cggtttatgg ctgattgcct
      481 ccacccatgg cgcaaacatg acgatgctgg gctttgccct gaccctggcg gcggccttgt
      541 cctgggcgct gtccaatctg gtggtgcgcc gcgccagcca ggccggccac cggccggaca
      601 ccctggcgct ggtggtgtgg gccagcctgg tcccgccgct gccgctgctg gcggcatccc
      661 tgctgtttga aggcccggcg cgcattggcg cggccttggc tgacctgagc tggcgcggcg
      721 gattggcggt gctgtacctg gcgtttatcg ccaccctggt gggctacagc gtatggagcc
      781 ggctgctgac ccgctacccg gctggcaagg tggcaccgtt ttcgcttctg gtgccggcag
      841 tggggctgtg ttcagcctcg tggctgctgg acgaacgttt gaccatgcag cagttggccg
      901 gcagcgcctg tgtgctgacc ggattgctca ttcatttatc aggcggttcc ctgtttgccc
      961 ggctgcgtcg gccggcatga cggaagcggg taagccgcca aggagcgaag catcatggcc
     1021 cagtggccca ctccgccgga aatcgagatt tactgcgtat tgcagccgcg ctttttgctg
     1081 ctggattttg ccggcgccgc cgaagctttc cggctggcca atgactatgg cggccgcttt
     1141 cgcctgcgct acgtcggccc ggacgccgcc ccggccaact cgctggggct gcccatccac
     1201 accatcgacc ccctgccgga cagcctgccg gaaggcgcct gggtgatgct gtccggggtg
     1261 accgaatccg ccaaggatta caccctgccg gccaccaaaa ccacccgcga ctggctcaag
     1321 cgcacattcc gccccaatca gcataccctg gtgtgcgtct gttccggcgc gctgctggcc
     1381 ggcatggccg gcctgctgga tggccggcgc tgcaccactc accacaccct caccgcccgg
     1441 ctggcccagc aatcgcccaa ggccgatgtg ctggaaaacc gcatttttgt ggaagacggc
     1501 ccgattcaca ccagcgcggg cgtcaccgcc ggggtggacc tggcgctgca cctgattgcc
     1561 cgccacgccg gcccggcggt ggcgctggca gtggcgcggg aaatggtggt ctacctgcgc
     1621 cgcgaaggca acgatgcgca gctgtcgccc tggctggcca accgcaacca tctgcacccg
     1681 gccgtgcacc gcgcgcaaga cctgatcgcg caagacccgc aacgccattg gccggtcagc
     1741 gagctggccg agcgcgtgca cgtcagcccg cgccatctgg cgcggctgtt tgcccagcac
     1801 gccggcttga gcatcatcga ctatcagcac cgcctgcgcg tggcgctggc gcgcgagcgg
     1861 ctggagcgcg gcgaaagtgt ggaatgcgcc gcccagcaag ccggctttgg ctcggcgcgc
     1921 agcctgcgcc gggtgtggca aaagttcgag cacctctcgc ccggcgcagt gcgcacccgc
     1981 gcgtagcacc gcttacaaaa ccctcctggc gttgtggtgc gacctgcttg gactgtcgcg
     2041 gtcgcgcgcc tcgccaggcg cgttgcgctg cattgcgtga gcgacgctca gtgcgcttca
     2101 agccgtgccc cataagcgcc gataagcccc atccgcatcg tattcagcgg cttgcttgtc
     2161 cgggttaaag cgccggccgc cgcgcgggtc ggttccccgg ccggcgatat acagccaatt
     2221 gccctgattg ctgtacacgt cataatccac caactgcgcc tcaaaccacg ccgcgccagc
     2281 gcgccagtcg tcgtcgagtt cgtgcaagag gtaactggcc gccacctggc gcaggcggtt
     2341 ggacagataa ccgctggcgg ccagctcgcg catggcggcg tccaccaggg gctggccggt
     2401 gcggccctca caccagcggg catagctggc cgggtgatga cgcggtgcag gcgcctgcgg
     2461 gttcaggccg ctgacgccat acaattgccg gccgcaggcc aggtgcagca gccggaagta
     2521 ttcccgccac aataattcaa agcgcaacca ctccgtgccc tcgctgacgc catattccgc
     2581 gtcgcgcgcc gccagcgccg cccacacctg gcgcaccgac aaggccccgc aggccagcca
     2641 gggcgaaaac ttgctggaat acgccgtgcc gctcaggcca ttgcgggtgg ctttgtatcg
     2701 ggcgatgtcc tcgccagcgc aatagccagc cagatgcgcc aacccggccg cttcgtccag
     2761 ccgccaggcc ggcaaggcat acggaaacgc gcagcccgac gccagcggcg ggcaaggtgc
     2821 ttcgctgggg ccgggcagca ccggcgcagc ggccggccag ggcggcaact ggctgagcgc
     2881 gggcagtggc gccagcggct gacagccggc gcggctgagt tgctggcgaa actggctgaa
     2941 caccttgggc aagtcggcca cggcaaacgg cagcgccgat ggctcgaaca gcccggattg
     3001 ccagtgctcg cgcacggtca ggccggccgc gcgcaaggcg gccacctcgg cgtcttcttc
     3061 tggcgcggca atgcgctcgc acaccacggt gtcagcgccc aactgacggg ccaaggctgg
     3121 caacgcctgc gccggctcgc cgtgcaacac cgccagccgg ctacccaagg cgcgcaagct
     3181 ggcgtccaag cccagccagg tttgcccgcg cgccgcctgt cggtgcggcc ccagacgcgg
     3241 cacgccccag cgggtagtgc tctccggcgg cagacacacc acaggacaca gcgcgctggc
     3301 gcgctcggcg gcccagcgca aggccggcgc gtcgtgcagc cgggcatcct gacgaaacca
     3361 gtaaatcatc acgctcatgt ctggggtctt tcttcgaggg tggctcacca catcgcgtag
     3421 aagcctgtgt agacacagca aggccagcag agtttactga gcgcgttacg caccagccag
     3481 caaggccacg cccagcagca tcagcgccga gccggccagc cgctgcgcgg tcaaggcctc
     3541 gcccagataa cgcgcgccca gccagctggc cagcagcatc gacacctcgc gcactggcgc
     3601 cacataactc aagggggcga tttgcagggc aaacaacacc agcgcatagg ccagcggcga
     3661 caacacgccc accagcagcg cggcgcgcca atggtcgcgc gcctggcggc gcatcgcttg
     3721 cggctgtcgc caggccagcg gcgccagcaa cacgctgcgc gccagcaggc tatagccata
     3781 aaacaccagc ggcggcatgg ccagggtttt gactgcgacg ccatccacca gggtgtaagc
     3841 ggcaatgcag gcgcccgtgg ccagccccca gcctatcccg gcgcgctggc gggctgggtc
     3901 ggcctcggcc tggccgccgc gactgagtaa gccgatgccg gcaatcagca aagcaatgcc
     3961 gccccaaccg cgcacgctgg gcgcttccgc cagccacagc gtggcgccca gcgccgacaa
     4021 caagggcccg ctgccgcgcg ccagcggata caccagcgaa aatggcgcgc gctggtagcc
     4081 gcgctgcaac accagcgaat acaccgcatg cagcagcgcg ctgcccagca ccaccgccag
     4141 catggccggc gtccaggtga gcgcctgcgc ctgccagatg gccagcggcg tcagccagca
     4201 caggctgacc acgccataca gccagacaaa cggcgcgccg ccgctggcca cctgtttggc
     4261 gatcagattc cacagcgcat gacacaccgc gccggccagc accagcgccc aggcaaacca
     4321 cgacatacac ccgccttgct tag
//
LOCUS       DAOPYX010000254         4319 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_26444_length_4319_cov_7.967402, whole genome shotgun sequence.
ACCESSION   DAOPYX010000254 DAOPYX010000000
VERSION     DAOPYX010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 4319)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4319)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4319
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26444_length_4319_cov_7.967402"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..257)
                     /locus_tag="PKN55_11515"
     CDS             complement(<1..257)
                     /locus_tag="PKN55_11515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011135591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX71916.1"
                     /translation="MPETTLGARQRAGVASGIVIFLSGYPMHTQMHAMLQHAPGGADT
                     LYWGETARPVPGPGQLRVRVRAAGVNRADIVQREGRYPPPPG"
     gene            complement(383..1342)
                     /gene="miaA"
                     /locus_tag="PKN55_11520"
     CDS             complement(383..1342)
                     /gene="miaA"
                     /locus_tag="PKN55_11520"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018150614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HNX71917.1"
                     /translation="MTDTLAPLPPAILLMGPTASGKTALALALARHIPVEIISVDSAL
                     VYRGMDIGAAKPSAAEQAQVPHHLIDIIDPPHAYSAAQFTQDARQLMAEITARGRVPL
                     LAGGTMLYFKALQDGLSDLPAANPAVRAELDQLAAERGWPALHAELARIDPATAARLA
                     PNDAQRIQRALEIHRLTGQPMSALIAERPHDPLPWRLLKIALLPSERSVLHQRIATRF
                     TQMLDAGLLDEVRALRAAWPELHLGLPSMRCVGYRQAWHYLDGDIDRQQLIDQGIAAT
                     RQLAKRQLTWLRGMEGLVEVDCLLEGEAVLERVLGAWEGMMVE"
     gene            complement(1356..3281)
                     /gene="mutL"
                     /locus_tag="PKN55_11525"
     CDS             complement(1356..3281)
                     /gene="mutL"
                     /locus_tag="PKN55_11525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HNX71918.1"
                     /translation="MSARIQRLPDHLVNQIAAGEVVERPAAALKELLENSIDAGASRV
                     TVDLAEGGIRLIRVNDNGGGIAADDLALALDRHATSKIRSLDDLEHVGTLGFRGEGLA
                     SIAAISRLTLTSRPPGAEMAWQVLAQDGALLPPEPAAHAEGTTVEVADVYFNTPARRK
                     FLKSPNTEYAHCEAAFERIALAHPEVEMTLRHNGKVSWRLARHTLAERVGQLLGNDFA
                     AGALSVDAQAAGLTLSGFAGSPTLGKSSRDAQYFFVNGRFVRDKLVNHAVRQAYHDVL
                     HHDRHPSYALFLTLDPAAVDVNVHPTKVEVRFREAQAVHQFIFHAVNKALAASKAGAD
                     SPAEGAADALDAPAASGARAPLLGSFEPPPRRPAGSSAGGGGSGGGLRYDSAPRQSRL
                     PLSVAEHGGGSGFYASLFAGVREAEQAASTPEHHTPSQPDAPPPAASLPTRHDDALPP
                     LGFALAQLHGVYILAQNADGLVVVDMHAAHERIVYERLKTQFDAQAIVQQPLLLPVAF
                     AADRLETAAAHDHAEALAALGLELAVLGPGQLAVRAAPALLKDADPVPLARAMLQELR
                     EHGGSQALTEHRNALLATMACHGAVRANRSLTLPEMNALLRDMEATERSGQCNHGRPT
                     WRALSMKELDGLFMRGQ"
     gene            complement(3278..3919)
                     /locus_tag="PKN55_11530"
     CDS             complement(3278..3919)
                     /locus_tag="PKN55_11530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011134897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HNX71919.1"
                     /translation="MELVSFLLDFILHIDRHLAELVAVYGPWIYLILFLIVFCETGLV
                     VTPFLPGDSLLFVAGTLAALGSMNIHLLVALLIVAAILGDACNYQIGRHAGARLFAKP
                     DSRIFRREYLNRTEAFYARHGGKTIVLARFVPIVRTFAPFVAGMGHMAYLQFARFNVL
                     GAVLWVCAFCYAGYLFGNMPVVKSNLSLLILGIIVVSILPGVIEIWRQRRGAA"
BASE COUNT          743 a         1402 c         1510 g          664 t
ORIGIN      
        1 cccggcggcg gcggatagcg gccttcgcgc tgcacaatat ccgcccgatt caccccggcg
       61 gcgcgcacgc gcacgcgcaa ctggccgggg ccgggcactg gccgcgcggt ttcgccccaa
      121 tacaaagtgt ccgccccacc gggggcatgt tgcagcatgg cgtgcatctg ggtgtgcatg
      181 ggatagcccg acaaaaaaat gacaatgcca gatgctaccc ccgcccgctg gcgcgcgcca
      241 agcgtcgtct caggcatggg ctatgcgtag gcaatggtaa gaatctggtg tggtcagctc
      301 ggcaacacac gccccagcgt agaccggtga gtaagagtcg cggtccgggt catcgacggc
      361 gttcatgccc aaacgccagc cctcactcca ccatcattcc ctcccacgcc cccaacaccc
      421 gctccagcac cgcctccccc tccagcaagc aatccacctc caccaaccct tccatcccgc
      481 gcagccaggt cagttggcgt ttggccagtt ggcgggtggc ggcgatgcct tggtcgatca
      541 gttgctggcg gtcgatgtcg ccgtccagat aatgccaggc ctggcggtag ccgacgcagc
      601 gcatcgaggg caggccgagg tggagttccg gccaggcggc gcgcagggcg cgcacttcgt
      661 ccagcaggcc ggcgtcgagc atttgggtga agcgggtggc gatgcgttgg tgcagcacgc
      721 tgcgctcgct gggcagcagg gcgattttca gcagccgcca gggcagcggg tcgtgtgggc
      781 gttcggcgat cagcgcgctc atcggctggc cggtcaggcg gtggatttcc agcgcgcgct
      841 ggatgcgctg ggcgtcgttg ggggccaggc gggcggcggt ggcggggtcg atgcgcgcca
      901 gttcggcgtg cagcgccggc cagccgcgtt ccgcggccag ttgatccagc tcggcgcgca
      961 cggcggggtt ggcggcgggc aggtcggaca ggccgtcctg cagcgccttg aaatacagca
     1021 tggtgccgcc ggccagcagc ggcacgcgcc cgcgcgcggt gatttcggcc atcaactggc
     1081 gggcgtcctg ggtgaattgc gcggcggaat aggcgtgcgg cgggtcgatg atgtcgatca
     1141 ggtggtgcgg aacttgcgct tgttcggcgg cgctgggttt ggcggcgccg atgtccatgc
     1201 cgcgatagac cagggcggaa tccacgctga tgatttccac cgggatgtgg cgggccaggg
     1261 ccagggccag ggcggttttg ccagaggcgg tggggcccat cagcaggatg gcgggcggca
     1321 ggggggcaag ggtgtcggtc atggtgggga aagagttact ggccgcgcat gaacaggccg
     1381 tcgagttctt tcatcgacaa ggcccgccag gtggggcggc cgtggttgca ctggccggag
     1441 cgctcggtgg cttccatatc gcgcagtagc gcgttcattt ccggcagggt caggctgcgg
     1501 ttggcgcgca ccgcgccgtg gcaggccatg gtggccagca gggcgttgcg gtgttcggtc
     1561 agcgcctggc tgccgccgtg ttcgcgcagc tcttgcagca tggcgcgcgc cagcggcacc
     1621 gggtcggcgt ctttcagcag ggccggggcg gcgcgcaccg ccagctggcc ggggccgagc
     1681 acggccagct ccagccccag cgcggccagc gcctcggcgt ggtcgtgggc ggcggcggtt
     1741 tccagccggt cggcggcaaa ggccaccggc agcagcagcg gttgctggac gatggcctgg
     1801 gcgtcgaact gggttttcag gcgctcgtag acaatgcgct cgtgggcggc gtgcatgtcc
     1861 accaccacca ggccatcggc gttttgcgcc aggatgtaca cgccgtgcag ttgcgccagg
     1921 gcaaagccca gcggcggcag ggcgtcgtcg tggcgggttg gcaggctggc ggcgggcggc
     1981 ggtgcgtcgg gctggctggg tgtgtggtgc tcgggcgtgc tggccgcctg ttcggcttcg
     2041 cgcacgccgg caaacaggct ggcgtaaaag ccgctgccgc cgccgtgctc ggccaccgac
     2101 aagggcaggc gcgactggcg cggcgcgctg tcgtagcgca ggccgccgcc cgaaccgccg
     2161 ccgcccgcgc tgctgccagc cggccggcgc ggcggcggct caaagctgcc gagcaagggc
     2221 gcccgcgcgc cgctggcggc gggagcgtcc agcgcgtcgg ccgcgccttc cgccgggctg
     2281 tcggcgccgg ctttgctggc ggccagcgcc ttgttcaccg catgaaaaat aaactgatgc
     2341 accgcctgcg cttcgcgaaa gcgcacttcc accttggtcg ggtggacgtt gacgtccacg
     2401 gcggccgggt ccagcgttaa aaacagcgca tagctggggt ggcggtcgtg gtgcagcacg
     2461 tcgtggtagg cctggcgcac ggcatgattc accagcttgt cgcgcacaaa gcggccgttg
     2521 acaaaaaaat actgcgcatc gcggctgctt ttgcccagcg tcgggctgcc ggcaaagccg
     2581 ctcagcgtca ggccggcggc ctgggcgtcc acgctcagcg cgccagcggc aaagtcattg
     2641 cccaatagct ggccaacgcg ctcggccagg gtgtgccgcg ccagccgcca gctgaccttg
     2701 ccattgtggc gcaaggtcat ctccacttct gggtgggcca gggcaatgcg ctcgaacgcg
     2761 gcttcgcagt gggcgtattc ggtgttgggg cttttcagga acttgcggcg cgccggggtg
     2821 ttgaagtaca catcggccac ttccaccgtg gtgccttcgg cgtgcgccgc cggctcgggc
     2881 gggagcagcg cgccatcctg cgccagcacc tgccaggcca tctcggcgcc gggcgggcgg
     2941 ctggtgaggg tcagccgcga gatggcggca atactggcca gcccctcgcc acgaaaaccc
     3001 agcgtgccga catgctccag atcgtccagc gagcgaatct tgctggtggc gtggcggtcc
     3061 agcgccaggg ccagatcgtc ggcggcaatg ccgccgccgt tgtcgttcac ccgaatcaag
     3121 cggatgccgc cctcggccag atccaccgtc acccggctgg cgccagcgtc gatgctgttt
     3181 tccagcaact ctttcagcgc agcggccggg cgctccacca cctcgccagc ggcaatctgg
     3241 ttgaccagat gatccggcaa gcgctggatg cgcgcactca tgccgccccc cgccgctgcc
     3301 gccagatttc aatcacgccg ggcaggatgg acaccacgat aatgcccagg atcaacaggc
     3361 tgagattgct cttcaccacc ggcatattgc caaacagata gccggcgtag cagaacgcac
     3421 acacccacag caccgcgccc agcacattga agcgggcaaa ttgcaggtag gccatatggc
     3481 ccatgccggc gacaaacggg gcaaaggtgc gcacaatcgg cacaaaacgc gccagcacaa
     3541 tggtcttgcc gccatggcgg gcgtaaaacg cctcggtgcg gttcaggtat tcacgccgaa
     3601 aaatccgcga atccggcttg gcgaacaagc gcgcgccggc atgacggcca atctggtaat
     3661 tgcaggcatc gcccagaatg gcggcgacaa tcagcaaggc caccagcagg tggatattca
     3721 tactgcccag cgcggccagc gtaccagcca caaacaacag cgaatccccc ggcaaaaacg
     3781 gcgtgaccac cagcccggtt tcgcaaaaca caatcaggaa caggatcaga tagatccacg
     3841 gcccgtacac agccaccaac tccgccagat ggcggtcgat gtgcagaatg aaatccagta
     3901 aaaaactgac gagttccata ggcttggcaa ggtctcgata acaatagagg gctaaaaaac
     3961 gatacacagc gccagaccgg cgcagattgg ccgatggggc gctgtggaaa cttgcctgga
     4021 gggggtcccc cccgcctgcg cggggacgac gcggtggaga gggaaacagc agcaggaaaa
     4081 gggagaaatg cggcgatgtg atatgcagca caccatcttg aatgtttacc ccagacaaca
     4141 cattcagacc acaagccgtc gcgcctagcc gctgttcacc cccgtgtgtc gcgccgagca
     4201 tcgcaggaag tcagggaggt ttccggcccg gctaagcgac gccgcaggcg gcgcgccatt
     4261 ccggggcggc cctgactttc gagaagcaca gggcagtcag cccgcagggc tgaccgcga
//
LOCUS       DAOPYX010000255         4285 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_26771_length_4285_cov_8.715839, whole genome shotgun sequence.
ACCESSION   DAOPYX010000255 DAOPYX010000000
VERSION     DAOPYX010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 4285)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4285)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4285
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26771_length_4285_cov_8.715839"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(111..1742)
                     /locus_tag="PKN55_11535"
     CDS             complement(111..1742)
                     /locus_tag="PKN55_11535"
                     /inference="COORDINATES: protein motif:HMM:NF024140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNX71920.1"
                     /translation="MKLNLFHRTTLGIALLLGLLLLCMATALLGLSRLHADTRQLADV
                     EVAKVDHASQAALMLSQVERDLSRILTREDKAAYARFKTAQNERRASLSHHFSELQRL
                     SYRPDSQALLAQVQARRQTLLAALGQITPLLDADDYAGARALYAQSGEAALSAYLQAV
                     DSYLALQKRVLAEHSAAASQTVAQFELWLQLIGGVSLLLAIAVGVWLVRGVKRPLGGD
                     PGVVSEALSRIAQGDLASQTPSGRAHPHSLLARLGGMRHGLRQLVGNIQGASGEVSSA
                     ARDLSASCAQIAAGAVEQGASTASMASAVEELGSNITSLAQSSRRVLDVAARAENLAS
                     ESDRMLGEAAGEIGKMIDTIDNSAQDVAQLASKTSEIGRIVGVINDIADQTNLLALNA
                     SIEAARAGEQGRGFAVVADEVRKLAEHTTRATAEIDEMIKSIQKQTRLAADNLLHGEE
                     VVGFGVRLVRDLVSPLRQLREGAGETRRELDGLMLALDEQTHAARHIGSHIERVASAA
                     EEFGLAARSSAQTASDLLGVVHRLDGEVAHFRLQG"
     gene            complement(1813..2052)
                     /locus_tag="PKN55_11540"
     CDS             complement(1813..2052)
                     /locus_tag="PKN55_11540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX71921.1"
                     /translation="MIYTPGQARCTELGERRQARREAHAEKIVIPAQTGIQPFDFAGF
                     CFWQTGFFDSISAALKIAAHFARHAAASNTLLSLF"
     gene            complement(2097..2273)
                     /locus_tag="PKN55_11545"
     CDS             complement(2097..2273)
                     /locus_tag="PKN55_11545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX71922.1"
                     /translation="MPITEAFSAHTIRQDLQRLSQSLGQLRLELDTVCRELSGEASPS
                     PAYDAALLPDWLRD"
     gene            2604..4244
                     /locus_tag="PKN55_11550"
     CDS             2604..4244
                     /locus_tag="PKN55_11550"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNX71923.1"
                     /translation="MSAAVRGGFVWRVGLGLGGLLLILLIVLALGGLGGWRMQAAAER
                     VADVDARKLRHGQQALFTLNALSRQVYPVLGGSPSQEDIDTLNKVFTASQREMQTQLD
                     SLAALGPSPEEQTMLAAIRQRHGEFNQATRGLLDLLAKDEYDAATSHFYALGVPALRE
                     DMMAIERFLRHQDQQFQQGAQASAAAAQHTQWSMAAASGVGVLAFIAVGMMILRSVSR
                     PLGGDPAQAGAAVASMADGQLHQPLPVRQGDGRSLMARLEAMRRQLGGTMGEIASNTR
                     AVEDAAQALSAECLRIAEGAQAQTLSTASIASAVEELDGAIHTLSSHTEHVVTQAHSA
                     ETLADQAQSQLDSAAARMGAMRDTLSQAHGDVGELADKTESIGQVVAVIQGIAEQTNL
                     LALNAAIEAARAGETGRGFAVVADEVRKLAEHTARATQDIAGTISAIQQQTRQAAERL
                     NHSQAGVDASVEEIAELHPAMEQLRAGAGKTRQAVEQLQSGLAEQASAAGLIGQQAER
                     VAQTAEAFVHGVRRSTDTADSLLGVVGRLNGQLAQFRV"
BASE COUNT          731 a         1324 c         1470 g          760 t
ORIGIN      
        1 cctactttct ttggcgaagc aaagaaagta ggtcggctgc gggacgaact cccgcagtca
       61 atcagccccc gcgcgcagcg cggcgcctaa cacctacctc aatccctccc tcacccctgc
      121 aacctaaaat gcgccacctc cccatccagc cgatgcacca cacccagtaa atcgctggcg
      181 gtttgcgccg agctgcgcgc ggccaggcca aactcttccg ccgcgctggc cacccgctcg
      241 atatggctgc cgatatggcg ggcggcgtgg gtttgttcgt ccagcgccag catcagcccg
      301 tccagttcgc ggcgggtttc gcctgcccct tcgcgcagtt ggcgcagcgg gctgaccagg
      361 tcgcgcacca ggcgcacgcc aaagcccacc acttcttcgc cgtgcagcag attgtccgcc
      421 gccagccggg tttgcttttg aatgctcttg atcatctcgt cgatttcggc ggtggcgcgg
      481 gtggtgtgtt cggccagttt gcgcacttcg tcggccacca cggcaaagcc ccggccttgc
      541 tcgcccgcgc gggcggcttc gatcgaggcg ttgagcgcca gcaggttggt ttggtcggca
      601 atgtcgttga tcacgccgac aatgcggccg atttcgctgg ttttgctggc cagttgcgcc
      661 acgtcctggg cggagttgtc gatggtgtcg atcatcttgc cgatttcgcc agcggcttcg
      721 cccagcatgc ggtcgctttc gctggccagg ttttctgcgc gggcggccac gtccagcacc
      781 cggcgcgacg attgcgccag gctggtgata ttgctgccca gttcttccac cgcgctggcc
      841 atcgacgcgg tggacgcgcc ttgctccacc gcgccagcgg caatttgcgc gcagctggcg
      901 gacaaatcgc gcgcggcgct ggagacttcg ccgctggcgc cctgaatatt gcccaccagt
      961 tggcgcaggc cgtggcgcat gccgcccagc cgggccagca ggctgtgcgg gtgggcgcgg
     1021 ccgctggggg tctggctggc caggtcgccc tgggcgatgc gcgacagggc ttcgctgacc
     1081 acgccggggt cgccgcccag cgggcgcttg acgccgcgca ccagccacac gccaacggcg
     1141 atggccagca gcaggctgac gccgccgatc agttgcagcc agagttcaaa ctgcgccacg
     1201 gtttggctgg cggcggcgct gtgttcggcc agcacgcgtt tttgcagcgc cagatagctg
     1261 tctacggctt gcagataggc gctcagcgcg gcttcgccgg attgggcgta tagcgcccgc
     1321 gcgccagcgt agtcgtcggc gtccagcagc ggggtgatct ggcccagcgc ggccagcagg
     1381 gtttgccggc gcgcctgcac ctgggccagc agcgcctggc tgtcggggcg gtagctcagc
     1441 cgttgcagct cgctgaaatg gtggctcagg ctggcgcggc gttcgttttg agcagttttg
     1501 aagcgggcgt aagcagcttt gtcttcgcgg gtgaggatgc gcgacaggtc gcgctccacc
     1561 tggctgagca tcaaggcggc ctggctggcg tggtccacct tggcgacttc gacgtcggcc
     1621 agttgccggg tgtcggcgtg caggcgggac aagcccagca gggcggtggc catgcacagc
     1681 agcaatagcc ccagcagcag ggcgatgccc agtgtggttc tgtggaataa attcagtttc
     1741 atgtcataac ctcgtcgccc ggcatgtgtg cccatgctcg gactgcgcgg ggcgcagtgg
     1801 gcgtagtgac tgctaaaaaa gggaaagcaa ggtgtttgaa gcagctgcgt ggcgggcgaa
     1861 atgcgcggcg atttttaaag ctgcgctgat tgagtcaaaa aatcccgttt gccaaaagca
     1921 aaacccagca aaatcaaatg gctggattcc agtctgcgcg ggaatgacga ttttttcagc
     1981 gtgtgcttca cgccgcgcct gacggcgctc gccaagctca gtgcagcgtg cctggccagg
     2041 cgtgtagatc acggcaaggc cgcgcaacaa cgccaggcgc gttttatcag ccgcgcttaa
     2101 tcgcgcagcc agtcgggcag cagggcggcg tcgtaggcgg gtgatgggct ggcttcgccg
     2161 gacagttcgc ggcaaacagt gtccagctcc aggcgcagtt ggccaagcga ttggctcagg
     2221 cgttgcaagt cctggcggat ggtgtgggca gaaaacgctt cggtgatggg catggagatt
     2281 ctcctgcaag acaaaggctg gcaaacgcgg ctggcggtgg gtgtcttgtg cggtcggcgt
     2341 ttggatacag ggatagatga taatggctat caattgtaaa gacaagcctg acaaaggtaa
     2401 attttttgac gagcatgtca aatcctgtta agcccgcagc cgaatattgc ccagcgccgc
     2461 ccgtcccggt gttatcgcaa gaaattgcct tttggatcat ttgcttgtat tccagtatgc
     2521 ccaaggtggt tttttgacca ttgggcaagc atccgggata atttgcccaa aattcacaca
     2581 atatggatca acaagggtgc ggtatgagtg cagcggtgcg tggcgggttt gtctggcggg
     2641 tgggcctggg tttggggggc ttgctgctga ttttgctgat tgtgctggcc ctgggcgggc
     2701 tgggcggttg gcgcatgcag gcggcggccg agcgggtggc ggatgtggac gcgcgcaaat
     2761 tgcgccatgg ccagcaggcc ttgtttacgc tgaatgcgct gtcgcgccag gtgtacccgg
     2821 tattgggcgg ctcgccatcg caggaggata tcgacacgct gaacaaggtg ttcaccgcca
     2881 gccagcgcga gatgcaaacc cagctcgaca gcctggcggc gctgggcccc agcccggaag
     2941 aacagaccat gctggcggcc atccgtcagc gccatggtga gttcaaccag gccacgcgcg
     3001 gcttgctgga cttgctggcc aaggacgaat acgacgccgc caccagccat ttttacgcct
     3061 tgggcgtgcc cgccctgcgt gaggacatga tggccatcga gcgctttttg cgccatcagg
     3121 atcagcaatt ccagcaaggc gcgcaagcca gcgcggcggc ggcgcagcat acgcaatgga
     3181 gcatggccgc cgccagcggc gtgggcgtgc tggcctttat cgcggtgggg atgatgattt
     3241 tgcgcagcgt cagccggccg ttgggcggcg atccggcgca ggctggcgcg gcggtggcca
     3301 gcatggccga tggccagttg catcaaccct tgccggtgcg ccagggcgat ggccgcagcc
     3361 tgatggcgcg gctggaggcc atgcgccgtc agttgggcgg caccatgggc gagattgcca
     3421 gcaacacccg cgcggtggaa gacgccgccc aggcgctgtc cgccgagtgc ctgcgcattg
     3481 ccgaaggcgc gcaggcgcaa accttgtcca ccgccagcat cgccagcgcg gtggaagagc
     3541 tggacggcgc catccacacc ttgtccagcc acaccgagca tgtggtgacg caggcgcaca
     3601 gcgccgaaac cctggccgac caggcgcaaa gccagctgga tagcgccgcc gcgcgcatgg
     3661 gggcgatgcg cgacacgctg tcacaggcgc atggggacgt gggcgagctg gcggataaaa
     3721 ccgaatcaat tggccaggtg gtggcggtga ttcagggcat tgccgagcaa accaatctgc
     3781 tggcgctgaa cgccgccatc gaggccgccc gcgcgggcga aacgggccgg ggttttgccg
     3841 tggtggccga cgaggtgcgc aagctggccg aacacaccgc gcgcgccacc caggatattg
     3901 ccggcaccat cagcgccatc cagcaacaaa cccgccaggc cgccgagcgc ttgaaccata
     3961 gccaggccgg ggtggacgcc agtgtggaag aaatcgccga gctgcatccg gcgatggagc
     4021 agctgcgcgc cggggcgggc aaaacccgcc aggccgtcga gcagctgcaa agcggcctgg
     4081 ccgagcaagc cagcgccgcc gggctgattg gccagcaggc cgagcgggtg gcgcaaaccg
     4141 ccgaagcctt tgtgcacggc gtgcgccgct ccaccgacac cgccgacagc ctgctgggcg
     4201 tggtgggccg gttgaatggc cagctggcgc agtttcgggt gtagcgccga gagccgctca
     4261 caaaaccctc ctggcgttgt tgcgc
//
LOCUS       DAOPYX010000256         4283 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_26792_length_4283_cov_9.444655, whole genome shotgun sequence.
ACCESSION   DAOPYX010000256 DAOPYX010000000
VERSION     DAOPYX010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 4283)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4283)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4283
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26792_length_4283_cov_9.444655"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1141)
                     /gene="dnaX"
                     /locus_tag="PKN55_11555"
     CDS             complement(<1..1141)
                     /gene="dnaX"
                     /locus_tag="PKN55_11555"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018749651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HNX71924.1"
                     /translation="MTYQVLARKWRPKRFADLVGQEHVVRALSNALIEQRLHHAYLLT
                     GTRGVGKTTIARILAKSLNCETGVGAEPCGVCAACRQIDAGRFVDLLEIDAASNTGID
                     NIREVLDNAQYAPSVGRFKVYIIDEVHMLSKSAFNAMLKTLEEPPAHVKFILATTDPQ
                     KVPVTVLSRCLQFSLRNMTPQQVAAHLAHVLDTEQVSYEPAALTLLGRAASGSMRDAL
                     SLLDQAIAYGIGEVREEGVRAMLGAVDRRYLFTLLAALADGDGAALMAEAEQLAARSI
                     GFDSALHEMAQLLYQITLAQTVPAALAADEPERDAILALAGVLPPSDVQLYYQIAITG
                     RRDLALAPDEHAGFNMALLRMLAFHPANLPASAPAPVSAAAVPPSA"
     gene            1367..1603
                     /locus_tag="PKN55_11560"
     CDS             1367..1603
                     /locus_tag="PKN55_11560"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018747067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HNX71925.1"
                     /translation="MAHTAPAPASFEEAVSRLETLIRDMESGQLPLEASLAAYQQGSE
                     LIRFCQARLAQAEQQLSVLENGELKPLTLEGADV"
     gene            1596..2492
                     /locus_tag="PKN55_11565"
     CDS             1596..2492
                     /locus_tag="PKN55_11565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014087783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HNX71926.1"
                     /translation="MSDAAFLGWMTAIQQEMETTLDALLPSADHEPRALNQAMRYATL
                     GGGKRVRALLTYAAGELGQAPAGALARAAAAVEMIHAYSLVHDDLPCMDDDVLRRGKA
                     TCHVAFDEATALLAGDALQTQAFAVLAAPELGVEPARQLDMLRLLAHASGPLGMAGGQ
                     AIDLASVGQALNLPQLEFMHALKTGALIRASVLLGALAGEALPAEQMGRLDHFSRVIG
                     LAFQVVDDVLDCEADSATLGKTAGKDAAHDKPTYVSLLGLAEARRMARELRDQAFAAL
                     EPFGARAERLRALADYIVVRSF"
     gene            2506..>4283
                     /gene="dxs"
                     /locus_tag="PKN55_11570"
     CDS             2506..>4283
                     /gene="dxs"
                     /locus_tag="PKN55_11570"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012697793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HNX71927.1"
                     /translation="MTSAARYPLLDTIDLPSDLRRLERRQLPQLARELREFLVESVSQ
                     TGGHFASNLGCIELTVALHYVFDTPHDRLVWDVGHQTYPHKILTGRRARMNTMRMKDG
                     LAGFPKREESEYDTFGVGHSSTSIGAALGMAAAARTQGQARKVVAVIGDGAMTAGMAF
                     EALNNAGAMDTDLLVILNDNDMSISPNVGALNHYLTKLMSGRFYAAVKEGSSKVLGMA
                     PPVHALVKRAEEHVKGMITPGALFEEFGFNYLGPIDGHDLDTLVETLGNVRKLKGPQF
                     LHVVTKKGQGYKLAEKDPIAYHGVSRFSPENGLAGGKGGGKPSYTQVFGDWLCDMAKQ
                     DRRLVGITPAMREGSGMVRFANEHADRYFDVGIAEQHAVTFAAGLACEGIKPVVAIYS
                     TFLQRAYDQLIHDVALQNLPVVFAIDRAGLVGADGPTHAGAFDLSFLRCIPNMVVMAP
                     SDENECRQMLYTAFTLDAPTAVRYPRGTGPGAHIQSDMTALPVGQGHIRRQGDGGVAF
                     LAFGSMVTPAMAAAEALNATVADMRFVKPLDEALVRQLAASHRLLVTVEENVIQGGAG
                     SAVLEALAAAGLDIPCLQLGLPDSYV"
BASE COUNT          723 a         1419 c         1428 g          713 t
ORIGIN      
        1 cggcgctggg cggcacggca gccgcagaaa ccggggccgg cgcgctggcg ggcagattgg
       61 ccgggtgaaa cgccagcatg cgcagcagcg ccatattaaa gccggcgtgt tcgtccgggg
      121 ccagcgccag gtcgcggcgg ccggtgatgg cgatttggta atacagctgc acatcgctgg
      181 gcggcagcac gccggccagg gccagaatgg cgtcgcgctc gggctcgtcg gcggccagcg
      241 ccgccggcac ggtttgcgcc agggtgatct gatacagcag ctgggccatt tcgtgcagcg
      301 ctgagtcaaa gccaatgctg cgcgcggcca gttgctcggc ctcggccatc agcgccgcgc
      361 cgtcgccgtc ggccagcgcc gccagcaggg tgaacagata acggcgatcc accgcaccca
      421 gcatggcgcg cacgccttct tcgcgcactt cgccaatgcc gtaggcaatg gcctgatcca
      481 gcagcgacag cgcatcgcgc atcgagccag acgccgcgcg gcccagcagg gtgagcgcgg
      541 ccggctcgta gctgacttgt tcggtatcca gcacatgcgc cagatgcgcc gccacctgct
      601 gcggggtcat attgcgcagc gagaattgca aacagcgcga cagcacggtg accggcactt
      661 tttgcgggtc ggtggtggcc aggatgaatt tgacgtgggc gggcggttct tccagcgttt
      721 tcagcatggc gttgaacgcc gacttggaca gcatgtgcac ttcatcgatg atgtacacct
      781 tgaagcggcc cacgctgggc gcgtactggg cgttgtccag cacttcgcgg atattgtcga
      841 tgccggtgtt gctggcggcg tcgatttcca gcaagtccac aaaccggccg gcgtcaatct
      901 gccgacaggc ggcgcacacg ccgcacggct cggcgcccac gccggtttcg cagttcaggc
      961 ttttggccag aatgcgggca atggtggttt tgcccacgcc gcgcgtgccg gtcagcaggt
     1021 aggcgtgatg caggcgttgt tcgatcaacg cattggacag ggcgcgcacc acatgctcct
     1081 ggccgaccag atcggcaaaa cgcttggggc gccacttgcg ggcaagaact tgataggtca
     1141 tgggcgcgga ttctacccga tttgccggcc ggccggcagt tggcgcgcgc agccagcagg
     1201 ggccgctggc cggcacgagg gcctggcatg accgacaaaa cccggccggg tggcccgagg
     1261 cggccacatc aataccaacc gcatggacag acgccacacg ccagaagggt tttgttagcc
     1321 cctctaagcg gctacaatcc gggggtttgc atttcttctg ttcatcatgg cccacaccgc
     1381 gcctgccccg gcttcatttg aagaagccgt ctcccgtctg gaaaccctga tccgcgacat
     1441 ggaaagcggc caactgccgc tggaagcctc gctggccgcc tatcaacaag gcagcgagct
     1501 gatccgcttt tgccaggcgc ggctggcgca ggccgagcaa cagctcagcg tgctggaaaa
     1561 cggcgaactc aaaccgctga ccctggaggg cgcagatgtc tgacgcggcg tttttgggct
     1621 ggatgaccgc catccagcaa gagatggaaa ccacactgga cgcgctgctg ccgtctgccg
     1681 accacgagcc gcgcgcgctg aaccaggcga tgcgctacgc cacgctgggc ggcggcaagc
     1741 gcgtgcgcgc gctgctcacc tacgccgctg gcgagctggg ccaggccccg gccggtgcgc
     1801 tggcgcgcgc ggcggcggcg gtggagatga ttcacgccta ttcgctggtg cacgacgacc
     1861 tgccgtgcat ggacgacgac gtgctgcgtc gcggcaaggc cacctgccat gtggcgtttg
     1921 acgaagccac cgcgctgctg gccggcgacg cgctgcaaac ccaggccttt gccgtgctgg
     1981 ccgcgcccga gctgggcgtg gagccggccc gccagctgga catgctgcgc ctgctcgcgc
     2041 acgcctcggg ccccttgggc atggccggcg gccaggcgat tgatctggcc tcggtgggcc
     2101 aggcgctgaa cctgccgcag ctggaattca tgcacgcgct gaaaaccggc gcgctgatcc
     2161 gcgcctcggt gctgctgggc gcgctggcgg gcgaggcgct gccggccgag cagatgggcc
     2221 ggctggatca tttttctcgg gtgatcggcc tggccttcca ggtggtggac gatgtgctgg
     2281 actgcgaagc cgacagcgcc acgctgggca aaaccgccgg caaagacgcc gcccacgaca
     2341 agcccaccta tgtcagcctg ctgggcctgg ccgaggcgcg gcgcatggcg cgcgagctgc
     2401 gcgaccaggc gtttgccgcg ctggagccat ttggcgcccg cgcggaacgc ttgcgcgcgc
     2461 tggctgacta tatcgttgtc cgttcgttct gatttgggtt tccgcatgac ctctgccgca
     2521 cgctatcccc tgctcgacac cattgacctg ccttctgacc tgcgccggct agagcgccgc
     2581 cagctgccgc aactggcgcg cgagctgcgc gagtttctgg tggagtcggt cagccagacc
     2641 ggcggccact ttgcctccaa cctgggctgc atcgagctga ccgtggcgct gcactatgtg
     2701 ttcgacaccc cgcacgaccg cctggtgtgg gacgtgggcc accagactta tccgcacaaa
     2761 atcctcaccg gccggcgcgc gcgcatgaac accatgcgca tgaaagacgg cctggccggc
     2821 tttcccaagc gggaagaatc cgaatacgac acctttggcg tcggccattc gtccacctcg
     2881 attggcgccg cgctgggcat ggccgccgcc gcccgcacac aaggccaggc acgcaaggtg
     2941 gtggcggtga ttggcgatgg cgcgatgacc gccggcatgg ccttcgaggc gctgaacaac
     3001 gccggggcga tggacaccga cctgctggtg atcctcaacg acaacgatat gtcgatctcg
     3061 cccaatgttg gcgcgctcaa ccactatctg accaagctga tgtccgggcg cttttacgcc
     3121 gcagtgaaag aaggctccag caaggtgctg ggcatggccc cgccggtgca tgcgctggtc
     3181 aaacgcgcgg aagaacacgt caagggcatg atcacccccg gcgcgttgtt tgaagaattt
     3241 ggctttaact atcttggccc gatcgacggc cacgacctgg acaccctggt ggaaaccctg
     3301 ggcaatgtgc gcaagctcaa gggcccgcag tttctgcacg tggtcaccaa aaaaggccag
     3361 ggctacaagc tggcggaaaa agacccgatt gcctaccacg gcgtgtcgcg cttcagcccg
     3421 gaaaacggcc tggccggcgg caagggcggc ggcaagccca gctacaccca ggtgtttggc
     3481 gactggctgt gcgatatggc caagcaggat cgccggctgg tgggcatcac cccggccatg
     3541 cgcgaaggct cgggcatggt gcgttttgcc aacgagcacg ccgaccgcta ttttgacgtg
     3601 ggcatcgccg agcagcacgc cgtcaccttt gccgctggcc tggcctgtga aggcatcaag
     3661 ccggtggtgg cgatttactc cacctttttg caacgcgctt acgaccagct gattcacgac
     3721 gtggcgctgc aaaacctgcc ggtggtgttc gccatcgacc gcgccggcct ggtcggcgcc
     3781 gacggcccca cccacgctgg cgcgtttgat ctgtcctttt tgcgctgcat ccccaatatg
     3841 gtggtgatgg cgccgtccga cgaaaacgaa tgccggcaaa tgctgtacac cgccttcacc
     3901 ctggacgcgc ccacggcagt gcgctacccg cgcggcaccg gccccggcgc gcatatccag
     3961 agcgacatga ccgcgctgcc ggtcggccag ggccatatcc gccgccaggg cgatggcggc
     4021 gtggcgtttc tcgcctttgg cagcatggtc accccggcga tggccgccgc cgaggcgctg
     4081 aacgccacgg tggctgatat gcgctttgtc aaaccgctgg acgaagcgct ggtgcgccag
     4141 ctggccgcca gccatcgcct gctggtcacc gtggaagaaa acgtcatcca gggcggcgcc
     4201 ggcagcgcgg tgctggaagc gctggccgcc gctgggctgg acatcccctg tctgcaactg
     4261 ggcctgcccg acagctatgt cga
//
LOCUS       DAOPYX010000257         4278 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_26846_length_4278_cov_7.421265, whole genome shotgun sequence.
ACCESSION   DAOPYX010000257 DAOPYX010000000
VERSION     DAOPYX010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 4278)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4278)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4278
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26846_length_4278_cov_7.421265"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..786
                     /gene="dmeF"
                     /locus_tag="PKN55_11575"
     CDS             <1..786
                     /gene="dmeF"
                     /locus_tag="PKN55_11575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012202396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family Co(II)/Ni(II) efflux transporter
                     DmeF"
                     /protein_id="HNX71928.1"
                     /translation="SMALLADGWHMATHAGAMLITVLAYRYAQRAAGEARHSFGAGKV
                     GDLAGFASALILIGVALIMAWESVARLLAPTPIEYGEAIAVAALGLAVNLASAWLLRD
                     DHAHHHHGHDHGHNHAGHHHHDHHDHNLRAAYLHVLADALTSVLAIVALLLGQRYGWS
                     WLDPVMGLVGAVVIVRWGRGLLHSTADVLLDAAPSGMTEAITQALGDDVGVLDLHVWQ
                     IGPGQHAAIVALSAARPASPAAYKARLTGVAGLAHVNVEVHAA"
     gene            complement(885..1604)
                     /locus_tag="PKN55_11580"
     CDS             complement(885..1604)
                     /locus_tag="PKN55_11580"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011136733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HNX71929.1"
                     /translation="MTTLFLADLHLSPDTPALNARFQAFLRAHAGQVEALYILGDLFE
                     FWLGDDDDRPFTRDCLSWLAAFARQTPLYVLPGNRDFLLGAGFCRASGATLLSEPHAL
                     TLYGRPYLLCHGDALCRDDVAYQRFRRKVRQRWRQWLFLALPLAWRARLVGAVRQETG
                     QAKMHKSAAIMDVNQDAVDELLNAAPSGSVLIHGHTHRPAHHQWTHHGQARQRWVIRD
                     WRDEGGGYLAVDADGVRALSC"
     gene            complement(1798..2301)
                     /locus_tag="PKN55_11585"
     CDS             complement(1798..2301)
                     /locus_tag="PKN55_11585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017508808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNX71930.1"
                     /translation="MIKLTTTLGDITLQLYPEKAPKTVANFEEYVRNGHYDGTLFHRV
                     INGFMIQGGGFASGMVQKPTGEAIPNEANNGLSNKPYTIAMARTMAPHSATAQFFINV
                     ADNAPLNFTAENLQGWGYCVFGEVVEGQDVVDKIKTVKTGRHGPHQDVPVEEVQIIKA
                     EIIENAA"
     gene            complement(2418..2984)
                     /locus_tag="PKN55_11590"
     CDS             complement(2418..2984)
                     /locus_tag="PKN55_11590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007342944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNX71931.1"
                     /translation="MRKLLTLVLFTLLPALVWAANPKVELTTNLGKIELELYPEKAPK
                     SVENFLRYVNDGQYTGTVFHRVIAGFMVQGGGFDKTMVERPTRAPIANEANNGLKNEP
                     GTLAMARTMAPHSATAQFFINVANNEFLNHRGQSMQGWGYAVFGRVTQGMDVVERIAK
                     TPTTTRGMYQDMPVSAVVIEKAVVLKTE"
     gene            3089..3427
                     /locus_tag="PKN55_11595"
     CDS             3089..3427
                     /locus_tag="PKN55_11595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003784152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family protein"
                     /protein_id="HNX71932.1"
                     /translation="MPVLYSVALLICSNVFMTFAWYAHLKNHAASPWWMAALASWGIA
                     LFEYLLQVPANRIGYGVLNLSQLKIIQEVISLSVFIPFAVLYMDQPFKWDYVWAALCI
                     LGAVYFMFRG"
     gene            complement(3516..4193)
                     /locus_tag="PKN55_11600"
     CDS             complement(3516..4193)
                     /locus_tag="PKN55_11600"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HNX71933.1"
                     /translation="MLLHVGALTLWPRHAPTPPTAGEPLAVRLLPAGPAVSAPASPLP
                     AAPTPPAPKHTATPEPRPTPPARKPEPRPAPTRPLSTPSPSATPAAAAPASAPAAAAS
                     SSSNNANSAAASTGPAAGAGGGEARTPARWQAAYLHNPPPDYPWQARQRGQQGVVWLS
                     VEVSAAGECAQASVLRSSGVSALDQAALAAVRRWRFVPARQGQTPVESKVEIPIRFKL
                     SDEGAEE"
BASE COUNT          669 a         1321 c         1460 g          828 t
ORIGIN      
        1 tcgatggcgc tactggccga tggctggcat atggccaccc atgccggggc aatgctgatc
       61 accgtgctgg cttatcgcta tgcccaacgt gccgcaggcg aggcgcgcca cagctttggc
      121 gccggcaagg tgggcgatct ggccggtttt gccagcgcgc tgatcctgat tggcgtggcg
      181 ctgatcatgg cctgggaaag cgtggcgcgc ttgctggcgc ccaccccgat cgaatatggc
      241 gaggccatcg ccgtggctgc gctggggctg gcggtcaacc tggcctcggc ctggctgcta
      301 cgcgacgatc acgcccatca tcatcacggc cacgaccatg gccacaacca tgccggccat
      361 catcatcacg atcaccacga ccacaatttg cgcgcggctt atctgcatgt gctggccgat
      421 gccctgacct cggtgctggc gattgtggcg ctcttgctgg gccagcgtta tggctggagc
      481 tggctggatc cggtcatggg gctggtgggg gcggtggtga ttgtgcgttg gggccgtggc
      541 ttgttgcaca gcacggcgga tgtgctgctg gatgcggcgc cgtccgggat gaccgaggcg
      601 attacccagg cgctgggcga cgatgtcggc gtgctggatt tgcacgtctg gcaaatcggc
      661 ccaggccagc atgccgccat tgtggcgctg agcgcagcgc gtccggccag cccggccgcc
      721 tacaaggcca gattgaccgg ggtggccggc ttggcgcatg tcaatgtgga agtccacgcg
      781 gcctgagggt gtgccgccct cctggtcagg tgtgcggccg caggcagtaa aaacacagta
      841 cggtcaggcc acgtcacaag gtcaggaggt tttgtgagcg gcggctaaca agacaatgcc
      901 cgcacgccgt cggcgtccac cgccagatag ccgccgccct cgtcgcgcca gtcgcggatc
      961 acccagcgct ggcgggcttg gccgtggtgt gtccattggt ggtgggccgg gcggtgggtg
     1021 tggccgtgga tcagcacgct gccgctgggc gcggcgttga gcaactcgtc cacggcgtct
     1081 tgattgacgt ccatgatggc ggcgcttttg tgcatcttgg cctggccggt ctcctggcgc
     1141 accgcgccca ccagccgcgc gcgccaggcc agcggcaggg ccagaaacag ccattgccgc
     1201 cagcgctggc gcaccttgcg gcgaaaccgc tggtaggcga catcgtcacg gcacagcgcg
     1261 tcgccatggc atagcagata cggccggcca tacagcgtca gcgcgtgcgg ctcgctcagc
     1321 agcgtggcgc cgctggcgcg gcaaaagccg gcgcccaata aaaaatcccg gttgccgggc
     1381 agcacataga gcggcgtttg ccgggcaaac gccgccagcc agctcaggca gtcgcgggtg
     1441 aatggccggt cgtcgtcgtc gcccagccag aattcgaata aatcgcccag gatatacagc
     1501 gcctccacct ggccggcgtg cgcgcgcaaa aacgcctgaa agcgggcatt aagcgccggt
     1561 gtgtcgggcg agaggtgcaa gtcagcaaga aacagggtgg tcacgaggcg gcaggcgggc
     1621 aaacagaaaa gaaaggcccg gcgcgccgca gggggcacac cgggcgggcg gcgtcatgcc
     1681 aagtcacggc agggcgcgcg caccggctta gcgttgctca caaaacccag cgcagcgggc
     1741 ctggcgtacg gcaaggtctt acaacaacac caggagggtt ttgtgagcgg cgcttaatca
     1801 ggcagcgttt tcgatgattt ctgccttgat gatctgcact tcttccaccg gcacatcctg
     1861 atgcgggccg tggcggccgg ttttcaccgt cttgatcttg tccaccacgt cctggccttc
     1921 caccacttcg ccaaacacgc agtagcccca gccttgcagg ttttcggcgg tgaagttcag
     1981 cggggcgttg tcggccacat tgatgaagaa ctgggcggtg gccgagtgcg gggccatggt
     2041 gcgcgccatg gcgatggtgt acggcttgtt ggacaggccg ttgttggctt cgttggggat
     2101 ggcttcgccg gtgggctttt gcaccatgcc gctggcaaag ccgccgccct ggatcatgaa
     2161 gccattgatc acgcggtgaa acagcgtgcc gtcgtagtgg ccgttgcgca cgtattcttc
     2221 gaagttggcc acggtcttgg gggctttttc cgggtacagt tgcagggtga tgtcacccag
     2281 ggtggtggtc agcttgatca tgtagagtct tcctcgtcaa aaaacagtcg ccgccggccg
     2341 aacgccgggg cgaaatggtc atcgtcaggt gggcaaacgc cgcccgcgcc cggccgtgaa
     2401 gccagggcgc ggcgggatta ttcggttttc agcaccacgg ctttttcaat caccacggcg
     2461 ctgaccggca tatcctggta catgccgcgg gtggtggtcg gcgtcttggc gatgcgctcg
     2521 accacgtcca tgccctgggt cacccgacca aacaccgcat agccccagcc ttgcatgctc
     2581 tggccgcgat ggttgagaaa ttcattattg gccacgttga tgaaaaattg cgcggtggcc
     2641 gaatgcgggg ccatggtgcg cgccatggcc agtgtgcccg gctcgttttt caggccgtta
     2701 ttggcctcgt tggcgatcgg cgcgcgggtg gggcgctcga ccatggtttt gtcaaagccg
     2761 ccgccctgca ccataaagcc ggcaatcacc cggtgaaaca ccgtgccagt gtattggcca
     2821 tcgttgacat agcgcagaaa gttttccacc gacttggggg ctttttctgg gtagagctcc
     2881 agctcgatct tgcccagatt ggtggtcagc tccactttcg ggttggccgc ccagaccagc
     2941 gcgggcagca gggtgaacag cacgagggta agcagcttgc gcatcagcaa tatcctgtca
     3001 gtacagcggt gggtttgggt atcatggcgg cgctcgccgc cccgtcgcgg cgcattctac
     3061 cacttctgcc cagactgttt ctgctgccat gcctgttctc tattctgttg ccctcttgat
     3121 ctgctccaat gtgttcatga cctttgcctg gtatgcccac ctgaaaaacc acgccgccag
     3181 cccgtggtgg atggcggcgc tggccagctg gggcattgcc ctgtttgaat acctgctgca
     3241 agtgccggcc aaccgcatcg gctatggggt gctgaacctg tcgcagctga agatcatcca
     3301 ggaggtgatt tcgctcagcg tgttcatccc gtttgccgtg ttgtatatgg atcagccgtt
     3361 caagtgggat tatgtctggg cggcgctgtg cattctgggg gcggtgtatt ttatgtttcg
     3421 ggggtaacct ggcattgttg tgccgtcttg ccgtacttgt tgcactgtct gcgcgcgcct
     3481 ggccaggcgc gctgcgctgc gttttatgcg cgcccttatt cctccgcacc ctcatcgctg
     3541 agtttgaagc gaatggggat ttccactttt gactccactg gcgtttgccc ctggcgggcc
     3601 ggcacaaagc gccagcgccg cacggcggcc agcgccgcct gatccagcgc cgacacgccg
     3661 ctgctgcgca acacgctggc ctgcgcgcat tcgcccgcag cagacacttc cacactcaac
     3721 cacaccacgc cctgctggcc gcgctggcgc gcctgccagg ggtagtcggg cggcgggttg
     3781 tgcaggtagg cggcctgcca acgggcgggg gtgcgcgctt cgccgccgcc cgcgcctgcc
     3841 gccgggcctg tgctggctgc ggctgagttg gcgttgttgc tgctggacga tgccgccgcc
     3901 gctggtgcgc tggctggtgc agccgcagcc ggggtggcgc ttgggctggg cgtgctcagc
     3961 gggcgggtgg gcgccggccg tggctcgggc ttgcgggcag gcggcgtcgg gcgcggctcg
     4021 ggggtggccg tgtgtttagg cgcaggtggc gtgggcgcgg cgggcaaggg gctggctggc
     4081 gcactgacgg ccgggccggc aggcagcagg cgcaccgcca acggctcgcc cgcggtgggc
     4141 ggcgtggggg cgtggcgcgg ccacagcgtg agtgcgccca catgcaacag cactgaaatc
     4201 accaacccca gcgcccagcg cctatcccat tcagcggacg cccgccatgt caaagacatc
     4261 gtaattaacc cttgattt
//
LOCUS       DAOPYX010000258         4262 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_26997_length_4262_cov_12.469931, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000258 DAOPYX010000000
VERSION     DAOPYX010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 4262)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4262)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4262
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26997_length_4262_cov_12.469931"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..611)
                     /gene="bioB"
                     /locus_tag="PKN55_11605"
     CDS             complement(<1..611)
                     /gene="bioB"
                     /locus_tag="PKN55_11605"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003780919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HNX71934.1"
                     /translation="MNASAPIAFHARPTPHPESATWTVDEIEALYSLPFLDLVFRAAA
                     LHREHFDPQRIQLSTLVSIKTGGCPEDCGYCPQSAHHDTPVENQPMMTVNEVLSAARA
                     AKDNGAGRFCMGAAWRGPKDADLDATLEMIREVKAMGLETCATFGLLQNGQAEKLKDA
                     GLDYYNHNLDTAPDKYADIISTRDYQDRLDTLGKVRAAGLSVC"
     gene            686..1390
                     /locus_tag="PKN55_11610"
     CDS             686..1390
                     /locus_tag="PKN55_11610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HNX71935.1"
                     /translation="MLSNLYRYFLNSAARLSQHCVLCGVGGQGWLCPPCRADLPRLAA
                     GRCPCCAMPGRQGALCAACRQSPPPWQALHVAHPYAWPLDALLHAFKYRQQLALARPL
                     AELMAEGVRHAPRPDWLAPIPLSAARLRERGFNQSEELARQLGKILRLPCHADTLLRT
                     RDTPAQAGLNRAGRLHNLAGAFAVNRPVEGRFIVLIDDVSTTGATLAEAAQSLARQGA
                     RRVEAWALMKALTHGA"
     gene            1443..1907
                     /locus_tag="PKN55_11615"
     CDS             1443..1907
                     /locus_tag="PKN55_11615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HNX71936.1"
                     /translation="MFTVVLYQPEIPPNTGNVIRLCANTGCELHLVKPLGFPLDDARM
                     RRAGLDYHEFATLVVHESWAECVAALAGRRIFAATTRGATRPDQAAFQPDDVLLFGPE
                     TRGLPTDVLAGIPPERCLRLPMRPDNRSMNLSNAVAVTVFEAWRQHGFAGGV"
     gene            2118..3086
                     /locus_tag="PKN55_11620"
     CDS             2118..3086
                     /locus_tag="PKN55_11620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014085591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HNX71937.1"
                     /translation="MQSVLRWLWLVTLTALLVACSTPNPASVSHKSTSGYKSARTTVP
                     VTKGGGYYKDDGPGDRRLPDNPDAIPDAVPVNEPLLTSANRPYTVMGQTYIPESNQSA
                     YRARGKASWYGKKFHGKRTASGEAYDMYEMTAAHPTLPIPSYARITNTSNGRSVVVRI
                     NDRGPFHSSRLIDLSYAAAYKLGYAGNGSAEVVVERVRAGDVEPTDSPVRLANVNPRD
                     DSVRVMNASLTAPMSGIADVARVPQPAGADGSGVWLQLGAFGTASNAESFRQRMLSLA
                     ASNDVEGRLDVVNRDGLYKVRIGPFSTMARARDVADRLNVQTVVMR"
     gene            complement(3185..4063)
                     /locus_tag="PKN55_11625"
     CDS             complement(3185..4063)
                     /locus_tag="PKN55_11625"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018746752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HNX71938.1"
                     /translation="MTGITTTQLTSLKKIYSGKVRDLYEIDDKRMLMIATDRLSAFDV
                     ILDEPIPEKGKILTAISNFWFDKLKDVVPNHLTGDRAEDLVSAADLPQVEGRGVVAKR
                     LTPVPVEAIVRGYLAGSGWKEYQQSGTVCGITLPAGLQEASQLPEPIFTPSTKAAVGD
                     HDENISFARCEEIVGAELAAKVRDTAIALYQAAAEYAASRGIIICDTKFEFGIDEHGV
                     LTLMDEALTPDSSRFWPADSYVAGSNPPSFDKQFVRDWLEASGWNKTPPAPAVPLDVR
                     EKTAAKYREALERLTG"
BASE COUNT          696 a         1351 c         1395 g          820 t
ORIGIN      
        1 cagcacacgc tcagcccggc ggcgcgcacc ttgcccaggg tgtccaggcg gtcttggtag
       61 tcgcgggtgg agataatgtc ggcgtatttg tccggggcgg tgtccagatt atggttgtag
      121 taatccagcc cggcgtcttt gagtttttcg gcctggccgt tttgcagcag gccaaaggtg
      181 gcgcaggttt ccaggcccat ggctttcact tcgcggatca tctccagcgt ggcgtccagg
      241 tcggcgtcct tggggccgcg ccaggcggcg cccatgcaaa agcggccggc gccattgtct
      301 ttggcggcgc gcgcggcgct gagcacctcg ttcaccgtca tcatcggctg gttttccacc
      361 ggggtgtcgt ggtgggccga ctgcggacag tagccgcagt cttccggaca gccgccggtc
      421 ttgatcgaca ccagggtgga caattgaatg cgctgcgggt cgaaatgctc gcggtgcagc
      481 gcggcggcgc gaaacaccaa atccagaaac ggcaggctgt acagcgcctc gatttcgtcc
      541 acggtccagg tggcggattc cgggtgcggg gtggggcggg cgtgaaaggc aatcggggca
      601 gaagcgttca tgagcggctc gtggcaagat gacaaaagcg tgggctgccg ggtggcagcc
      661 gtcaccatgg tgggagcttt gcctgatgct gtcaaattta taccgctatt ttttgaacag
      721 cgccgcccgc ctcagccagc attgcgtgct gtgcggcgtc ggcggccaag gttggctgtg
      781 cccgccttgc cgcgccgacc tgccccggct ggccgccggg cgctgcccgt gttgcgcgat
      841 gccggggcgg cagggcgcgc tgtgcgccgc ctgtcggcag tcgccgccgc cgtggcaggc
      901 gctgcatgtg gcgcatcctt atgcctggcc gctggatgcg ctgttgcatg cgttcaaata
      961 tcgccagcaa ctggcgctgg cgcgaccgct ggccgagctg atggcggaag gcgtccgcca
     1021 cgcgccgcgt ccggactggc tggcgccgat tccgctcagc gccgcgcggc tgcgcgagcg
     1081 cgggtttaat caaagtgagg aactcgcccg ccagttgggt aaaatactgc ggctgccttg
     1141 ccatgccgac acgctgttgc gcacgcgcga caccccggcg caggccgggc tgaaccgcgc
     1201 tggccgcttg cacaatctgg ccggcgcctt tgccgtcaat cggccggtcg agggccgttt
     1261 tatcgtgctg atcgacgatg tgagcaccac cggcgccaca ctggccgagg cggcgcaaag
     1321 ccttgccagg caaggcgcga gacgggtcga ggcctgggcg ctgatgaagg cgttgacaca
     1381 cggcgcttga ctaacttgtt gatcccgcgg aataatccgt cgcaaccaaa gtttttgacc
     1441 aaatgtttac agtcgttttg taccagccgg agattccgcc caacaccggg aacgtgattc
     1501 gtttatgtgc caacaccggc tgcgagctgc atttggtcaa gccgcttggc tttccgctgg
     1561 acgacgccag aatgcgtcgc gccggcctcg attatcacga gtttgctacc ctggtggtgc
     1621 atgaaagctg ggcggagtgt gtggcggccc tggccggaag gcgcattttc gccgccacca
     1681 cacggggcgc cactcgcccc gatcaggctg cgtttcaacc ggacgacgtc ttgttgttcg
     1741 gcccggaaac ccggggcctg cccaccgacg tgctggcggg catcccgccg gaacgctgcc
     1801 tgcgcctgcc gatgcgaccg gacaaccgca gcatgaatct gtccaacgcc gtggccgtca
     1861 ccgtctttga agcgtggcgg caacacggat tcgccggcgg cgtgtaaccg cgccgacctc
     1921 tctttacagg tccaggatgt gctttacaga cgcgccatcg cgacaggcgc gcgctgcgtc
     1981 acaccctgcg tgcgcccgag cgcgacatgg cctggcttcc ctggcgacat cccgcagtgg
     2041 atgtcgggac gccgtttctt ctggccgtgt cgtatcccga agacgtgttg gcacacaaca
     2101 gcaaaaggga gccctagatg caatctgtcc tccgctggct gtggttagtg accttgaccg
     2161 ccttgctggt cgcctgttcg acgccgaatc cggcgtctgt cagtcacaaa tccacctctg
     2221 ggtataaatc tgcccgcacc acggtcccgg tgaccaaggg cggcggctat tataaagacg
     2281 acggtcccgg agaccgccgc ctgcccgaca acccggacgc cattccggac gccgtgccgg
     2341 tcaatgagcc cttgctcacc tcggccaatc gtccgtatac ggtgatgggc cagacctaca
     2401 ttccggaaag caaccagtcc gcctaccgcg cccgcggcaa ggcctcttgg tatggcaaga
     2461 agttccatgg caagcgcacc gcctctggcg aggcgtacga catgtacgaa atgaccgccg
     2521 cccaccccac gctgcccatc cccagctacg cccgcatcac caacaccagc aatggccgtt
     2581 cggtggtggt gcgcatcaac gaccgtgggc cgtttcactc gtcccggctg attgatctgt
     2641 cctacgccgc cgcctacaag ctgggttatg ccggcaatgg cagcgccgaa gtggtggtcg
     2701 agcgggtgcg tgcgggtgat gtcgagccta ccgacagccc ggtgcgtctg gccaacgtca
     2761 acccgcgtga tgacagcgtg cgggtgatga acgccagcct gaccgcgccg atgtccggca
     2821 ttgccgatgt ggcgcgcgtg ccgcaaccgg ctggcgccga tggcagtggc gtatggctgc
     2881 aactgggcgc ttttggcact gcgagcaatg ccgaaagctt ccgtcagcgc atgttgtcgc
     2941 tggcggccag caacgacgtg gaaggccgcc tggatgtggt caaccgcgat ggcctgtaca
     3001 aggtgcgcat cgggccgttc tccaccatgg cgcgcgcgcg cgatgtggcc gatcgtttga
     3061 acgtgcaaac cgtggtgatg cgctaagcgt tttgccgctg tgccataaaa aaacccgcac
     3121 ctgaatcagg ttgcgggttt ttttatggct gccaggcggg ttttgtcagc gcgcttggcg
     3181 tcgcttagcc ggtcaggcgc tccagcgctt cacgatactt ggcggcggtt ttttcccgca
     3241 catccagcgg cacggccggg gccggcgggg ttttgttcca gcccgaggct tccagccagt
     3301 cgcgcacaaa ctgcttgtcg aacgacggcg ggttgctgcc ggccacatag ctgtccgccg
     3361 gccagaagcg gctggaatcc ggggtcagcg cttcgtccat cagcgtcagc acgccgtgct
     3421 cgtcgatgcc gaattcaaac ttggtgtcgc agatgatgat gccacggctg gcggcgtatt
     3481 ccgccgccgc ctggtacagg gcgatggcgg tgtcgcgcac cttggcggcc agctcggcgc
     3541 cgacgatttc ttcgcagcgg gcaaagctga tgttttcatc gtggtcgccc accgccgcct
     3601 tggtggacgg ggtgaaaatc ggctcaggca gctggctggc ttcttgtaag ccggcgggca
     3661 gggtgatgcc gcacaccgtg ccggactgct ggtattcctt ccagcccgaa ccggccagat
     3721 aaccgcgcac aatggcttcc accggcaccg gcgtcagccg cttggccacc acgccgcggc
     3781 cttccacttg cggcaaatcg gccgccgaca ccagatcttc ggcgcggtcg ccggtcaggt
     3841 gattgggcac cacgtccttg agtttgtcga accagaaatt ggaaatggcg gtgaggatct
     3901 tgcctttttc cgggatgggt tcgtccagaa tcacgtcaaa cgccgacagg cggtcggtgg
     3961 cgatcatcag catgcgctta tcgtcgattt cgtacaggtc gcgcactttg cccgaataga
     4021 tttttttcag gctggtcagt tgggtggtgg tgatgccggt catgagggcc tcaattcaaa
     4081 ttcaatgatg ggcaggcgcg ccgcccgccc ggaaaagcaa aatgcccctg gctcggcacg
     4141 gccggtctgg cggacaggct gccgccaggg gcgggggatg tcaacacacg ctcagcggcc
     4201 ttacagggtt tccttcaagg cgcgcgcctg ttccagcgcg gcgtccacgt ctgtggccag
     4261 ca
//
LOCUS       DAOPYX010000259         4247 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_27128_length_4247_cov_7.825143, whole genome shotgun sequence.
ACCESSION   DAOPYX010000259 DAOPYX010000000
VERSION     DAOPYX010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 4247)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4247)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4247
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27128_length_4247_cov_7.825143"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..261
                     /locus_tag="PKN55_11630"
     CDS             <1..261
                     /locus_tag="PKN55_11630"
                     /inference="COORDINATES: protein motif:HMM:NF025840.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DarT ssDNA thymidine ADP-ribosyltransferase
                     family protein"
                     /protein_id="HNX71939.1"
                     /translation="RIIHRDNLPWVLDHGLHCGNSAVKAPGWVSIGNRYQAEALIHRH
                     VPITGLLGIVCYTEALKQDIEQEILARHLSLPVHVRTGWYFS"
     gene            258..1316
                     /locus_tag="PKN55_11635"
     CDS             258..1316
                     /locus_tag="PKN55_11635"
                     /inference="COORDINATES: protein motif:HMM:NF013798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macro domain-containing protein"
                     /protein_id="HNX71940.1"
                     /translation="MITFTQGNLLEARVEALVNTVNTVGVMGKGIALMFKERFAENFR
                     RYAAACKAREVQTGKMFVTPVHELDGPRWIVNFPTKQHWRSPSRLEWVQEGLQDLRRF
                     LLEQHIQSVAIPPLGAGNGGLEWAKVREQITQTLGDLDIEILVFEPTSQYQNVAKRTG
                     VEKLTPARALIAELVRRYWVLGMECSLLEIQKLAWFLEREIEKRGLTSLDLRFNAHKY
                     GPYADRLRHLLEGLDGSYLHSDKRISDATPLEVIWFDDTRKDLVHRYLQSEAKAYAHA
                     LESTTALIDGFESPFGMELLATVDWLLAREGVAPTVPALREGLRHWRGGSDAAARKDR
                     LFDDRALGIALERLRGFA"
     gene            1316..1885
                     /locus_tag="PKN55_11640"
     CDS             1316..1885
                     /locus_tag="PKN55_11640"
                     /inference="COORDINATES: protein motif:HMM:NF018154.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HNX71941.1"
                     /translation="MPTCWIIAGPNGAGKTTFALEYLPQVAQCSRFVNADLIAAGLSP
                     LAPERELLAASRLFLGEIEACITQREDFAFETTLAGRSYLKLVRRLQTAGWRVELIYL
                     ALPSMEMSKLRVAERVAHGGHNIPVADIERRFARSLGNLLYSFSTQVDACRCFMNSDA
                     SPELVFEQQGEVRNVLHMPYYQHLLKEAE"
     gene            1885..2058
                     /locus_tag="PKN55_11645"
     CDS             1885..2058
                     /locus_tag="PKN55_11645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX71942.1"
                     /translation="MTITSVPPSPQAQAMLKALQTAVANSLDKKQKLGQYAVIWQNGQ
                     PVQTGSDAPKATQ"
     gene            complement(2075..3028)
                     /gene="paaX"
                     /locus_tag="PKN55_11650"
     CDS             complement(2075..3028)
                     /gene="paaX"
                     /locus_tag="PKN55_11650"
                     /inference="COORDINATES: protein motif:HMM:TIGR02277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation operon negative
                     regulatory protein PaaX"
                     /protein_id="HNX71943.1"
                     /translation="MSAELTPIQQRLEDFRQQGRVQAGSLIVSVFGDAVMPRGSRIWL
                     GSLIRLLEPLELNERLIRTSVFRLAKEEWLCSEPAGRRTDYLLAPAGQRRFAEASRHI
                     YAARAPQWDRRWRLIMLVGELAGKPREQLKRALFWQGFGMLGSDCFVHPSVDLTNAFD
                     ALIADGLGELLGRLKPLIAADTPYGSAASDLDMVHAAWNLNDLAAMYASFVQRYQPVL
                     AQLRADSEHALDEESAFLLRILLIHDYRRLLLRDPELPDVLLPGDWPGHQARQLCREI
                     YHRLLPASERHLDRHFQLANGQTPEASPTVYARFGEVDPLG"
     gene            complement(3288..>4247)
                     /locus_tag="PKN55_11655"
     CDS             complement(3288..>4247)
                     /locus_tag="PKN55_11655"
                     /inference="COORDINATES: protein motif:HMM:NF012402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HNX71944.1"
                     /translation="ALPNNCRAGACGECKVKVSAGHFDQGMVLDMALSQEERRQGYGL
                     MCMAKPISDELTIEWGTEDAKPKLFPPREDALFVLVDKRQVAERVVELRLRPVGQPIR
                     YWPGQYVMLGNPRADIPLRAYSIANAPRPDGELVLQVARADDGVTSAWVHEQLNIGDS
                     LKVSGAYGTFIGDPSVNTPVLCLAAGTGLAPILALTEAALRRGYRGKVTLVFSARTLA
                     DVYSLGLLAWWRAKHRNFDYHITLTRDQQDGCRHGRIPELLPSLCADLSKHTVFIAGS
                     PAFVEACQTAARSQGAPAEQIHSEGFFAQQQRLQPDPPPVAVF"
BASE COUNT          780 a         1264 c         1419 g          784 t
ORIGIN      
        1 cgcatcatcc accgcgacaa tctgccgtgg gttctggatc acggcttgca ctgcggcaac
       61 agcgccgtca aagcccctgg ctgggtaagc atcggcaatc gttatcaagc cgaggcgttg
      121 attcaccgcc atgtgccgat caccgggctg ctgggcattg tctgctacac ggaagcattg
      181 aaacaggata ttgaacagga gattctggcg cgccatctga gcctgccagt gcatgttcgc
      241 acagggtggt atttctcatg atcacattca ctcaaggcaa tttgctggag gcccgcgtcg
      301 aggcgctggt caacacggtc aacaccgtcg gggtgatggg caagggcatc gcgctgatgt
      361 tcaaggagcg ctttgccgag aacttccgcc ggtatgctgc cgcgtgcaaa gccagggaag
      421 tgcagaccgg caagatgttt gtgacgcctg tacacgaact ggacggtccg cgctggatcg
      481 tcaattttcc caccaagcag cactggcgtt caccttcacg gctggagtgg gtgcaggaag
      541 gtttgcagga tttgcggcgc tttttgcttg agcagcacat ccagtctgtc gctattccgc
      601 ccttgggcgc aggcaatggc ggcctggagt gggctaaggt gcgcgaacag atcacccaga
      661 cgctgggcga tttggacatt gagattttgg tgtttgaacc caccagtcaa tatcaaaacg
      721 tggccaagcg gacaggggtg gaaaagctga cgccagctcg tgcattgatt gctgagttgg
      781 tgcgccgtta ctgggtgctg ggcatggagt gcagcttgct ggaaatccag aagcttgcct
      841 ggtttctgga gcgtgaaatc gaaaagcgcg gattaacgtc gctggatttg cgttttaacg
      901 cccacaaata cggcccatac gcagatcgcc tgcgccactt gctggaaggg ctggacggca
      961 gctatttgca ttctgacaag cgcatcagcg atgcaacccc cctggaggtg atctggtttg
     1021 acgatacacg aaaagacctt gttcatcgct atcttcagag tgaagccaag gcctacgccc
     1081 acgcgctgga atccaccacg gcgctgattg acggctttga gtcgccgttt ggcatggagc
     1141 tgttggccac ggtggactgg ctgctggccc gtgaaggcgt ggcgcccacg gtgccggcgc
     1201 tgcgcgaagg gctgcgccac tggcgcggtg gctcagatgc cgctgcccgt aaggaccgac
     1261 tcttcgatga ccgagcgctg ggcattgccc tagaacgcct gcgggggttt gcctgatgcc
     1321 cacctgctgg atcatcgccg gccccaacgg cgcgggcaaa accactttcg ccctggaata
     1381 cctgccgcag gtggcgcagt gctcgcgctt tgtgaatgcg gatctgattg ccgccggttt
     1441 gtcgccgctg gcgcccgaac gcgaattatt ggccgccagc cgcttgtttt tgggcgagat
     1501 cgaagcttgc atcacgcagc gtgaagactt tgcctttgaa accacactgg ccgggcgcag
     1561 ttatctgaag ctggtccgtc gtctgcaaac agcgggctgg cgggttgaac tgatttacct
     1621 ggcgctgccc agcatggaaa tgtccaaact gcgcgtcgcc gagcgggtgg cgcatggcgg
     1681 ccacaatatc ccggtcgccg acattgaacg tcgctttgcc cgtagcctgg gcaacttgct
     1741 ctacagcttc agcacacagg tggatgcctg ccgctgcttc atgaacagtg atgcctcacc
     1801 agaactggtg tttgaacagc agggtgaggt gcgcaatgtc ctgcacatgc cgtattacca
     1861 acatttgttg aaggaggctg agtgatgacc atcacatccg tcccgccgtc accccaggcg
     1921 caagccatgc tcaaagcctt gcagaccgcc gtggccaaca gcctggataa aaagcagaag
     1981 ctcggacaat acgccgtgat ctggcagaac ggccagcccg tgcaaaccgg cagcgatgcg
     2041 ccaaaggcga cgcagtaagc acggcgacac gcgcctaccc cagcggatcc acctcgccaa
     2101 accgcgcata caccgtcggc gaggcttccg gggtttggcc gttggccagc tgaaaatgcc
     2161 ggtccaggtg gcgttcggag gcgggcagca ggcggtggta gatttcgcgg cacagttgcc
     2221 gggcctggtg gccgggccag tcgccgggca gcagcacgtc gggcagttcc gggtcgcgca
     2281 gcagcaggcg gcggtagtcg tgaatcagca ggatgcgcag cagaaaggcg ctttcttcgt
     2341 ccagcgcgtg ttcgctgtcg gcgcgcagtt gcgccagcac cggctggtag cgctggacaa
     2401 agctggcgta catggcggcc aggtcgttga ggttccaggc ggcgtgcacc atgtccaggt
     2461 cgctggcggc gctgccgtag ggggtgtcgg cggcgatcag cggcttgagc cggcccagga
     2521 gctcgcccag gccgtcggcg atcagcgcgt caaaggcgtt ggtcaggtct acgctggggt
     2581 gcacaaaaca atcgctgccc agcatgccaa agccctgcca gaacagcgcg cgcttgagtt
     2641 gttcgcgcgg tttgccggcc agctcgccca ccagcatgat cagccgccag cggcggtccc
     2701 attgcggcgc gcgcgcggcg tagatatggc gtgaggcttc ggcaaagcgg cgctggccgg
     2761 ccggggccag caggtagtcg gtgcgccggc cggccggctc gctgcacagc cattcttctt
     2821 tggccaggcg aaacaccgag gtgcggatca agcgttcgtt cagctccagc ggctccagca
     2881 ggcggatcag gctgcccagc cagatgcggc tgccgcgcgg catgacggcg tcgccaaata
     2941 ccgaaacaat cagcgagccg gcctgcaccc ggccttgttg gcgaaaatct tcgagtcgtt
     3001 gctggatggg ggtaagctcg gcggacattg cgggtcgatc tgggaaggtt ggggtgggcg
     3061 ggcaaagcgg gagtttactc gcagtggcgg gctgggggtg aagtgtgaat cagggctgcg
     3121 ctgatgccca gcgtggctaa ggctggattt tgggcggggc ttcgccccgc cacacgggat
     3181 aaacggcggc taagcgcaac aatgtcagga gttttgccac cattcagcca cctcagccat
     3241 caccctgcgc agcaaagcgc tgttggccag ctcacccaca cgccccctca aaacaccgcc
     3301 acgggcggtg ggtcgggttg caggcgttgt tgctgggcaa aaaagccctc gctgtgaatc
     3361 tgctcggccg gcgcgccctg gctgcgggcc gcggtctggc aggcctcgac aaaggccggg
     3421 ctgccggcga taaacaccgt gtgtttggac aagtcggcgc acaggctggg caagagctcg
     3481 gggatgcggc cgtggcggca gccgtcttgc tggtcgcggg tgagggtgat gtggtagtca
     3541 aaattgcggt gcttggcgcg ccaccaggcc agcaggccca ggctatacac atcggccagg
     3601 gtgcgggcgg agaacaccag ggtcaccttg ccgcgatagc cacggcgcag ggcggcttcg
     3661 gtcagcgcca ggatcggcgc caggccggtg ccggcggcca ggcacagcac cggggtgttg
     3721 accgaggggt cgccaataaa cgtgccgtaa gcgccagaca ctttcaggct gtcgccgata
     3781 tttaattgtt catgcaccca ggcgctggtc acgccgtcgt cggcgcgggc cacttgcagc
     3841 accagttcgc catccgggcg cggggcgttg gcgatggaat aggcgcgcag cgggatgtcg
     3901 gcgcgcgggt tgcccagcat cacatactgg ccgggccagt agcggatggg ctggcccacc
     3961 gggcgcaggc gcagctcgac cacccgttcg gccacctggc gtttgtccac cagcacaaac
     4021 agcgcatcct cgcgcggcgg aaacagcttg ggcttggcgt cttcggtgcc ccattcgatg
     4081 gtgagttcgt cagaaatcgg cttggccatg cacatcaggc catagccctg gcggcgctct
     4141 tcttgcgaca gcgccatgtc cagcaccatg ccctggtcaa aatggccggc gctgaccttg
     4201 accttgcatt cgccgcaggc gccggcgcgg cagttgttgg gcagggc
//
LOCUS       DAOPYX010000260         4217 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_27398_length_4217_cov_10.061749, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000260 DAOPYX010000000
VERSION     DAOPYX010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 4217)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4217)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4217
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27398_length_4217_cov_10.061749"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..257
                     /locus_tag="PKN55_11660"
     CDS             <1..257
                     /locus_tag="PKN55_11660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX71945.1"
                     /translation="PKFKQGEDYAQVRSKLLNAGWKPRRMPDADQCMQGDDRCQGRPE
                     MEACAGSGRANCRFAWKRAGALLTIFTVGSPAVFEAASNE"
     gene            complement(428..775)
                     /locus_tag="PKN55_11665"
     CDS             complement(428..775)
                     /locus_tag="PKN55_11665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX71946.1"
                     /translation="MGKKKLIIDTNLLLLLVIGAVEGGRHIQNSKRLNKFTLQDYDAI
                     LDVMASHQEICITHYIATEVSNLIDLTGYASRLAYETARILFSQFTQILPTLLQIAPK
                     THFWRMASPIAHW"
     gene            complement(780..1082)
                     /locus_tag="PKN55_11670"
     CDS             complement(780..1082)
                     /locus_tag="PKN55_11670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX71947.1"
                     /translation="MIDFDEAIRIAQENVVKLVKGAKNLVLEGALISADNTRYEVTIS
                     YELEGDDPPPAPDSAKDNAANLMALIAIMKKRRECKVFFVDSHSGQFRGFKRSTEG"
     gene            complement(1105..1956)
                     /gene="rpoH"
                     /locus_tag="PKN55_11675"
     CDS             complement(1105..1956)
                     /gene="rpoH"
                     /locus_tag="PKN55_11675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HNX71948.1"
                     /translation="MSATFALPIPAAAGSLEQYIQTVNSVPMLSQEEETELATRLHQH
                     NDLDAARRLVMSHLRVVVSVARGYSGYGLQQADLIQEGNIGLMKAVKRFEPQRGVRLF
                     SFAIHWIKAEIHEFILRNWRLVRVATTKPQRKLFFNLRSMKSGFAALSDKEARQIAED
                     LGVKPEEVREMETRMSGQDVALMADDNDDEGGYAPIDWLADAENEPTRQIEKKAFDRL
                     QREGLEHALSTLDARSRRIVESRWLADDGGLTLHDLAAEYGVSAERIRQIEAKALQKM
                     KQALVVA"
     gene            complement(2242..3150)
                     /gene="ftsX"
                     /locus_tag="PKN55_11680"
     CDS             complement(2242..3150)
                     /gene="ftsX"
                     /locus_tag="PKN55_11680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HNX71949.1"
                     /translation="MKHLLYLHGRAARQALRTLLRQPLGSLLSLFMLAVAISLPLGLY
                     LTVASLANWSNGLAASPQITLFMELNADRADVETVNQTLKQHALVHSHRFVSKESALK
                     DLMARNGLAEVTDGLAGNPLPDAFIVSPKDTEPEALERLQKELSGLPMVETSQFDAGW
                     ARKLHSLLTLGHQLTILLGAALGIALVLASSNTIRLQILARRNEIEVSKLIGATDGFI
                     RRPFMYFAVFQGVLAALVAWGLSAWLIATANPAVAELAALYNNHATLRQLAPEEVAAL
                     LLGVATLAALGARLATGHVLREMEPS"
     gene            complement(3147..3800)
                     /gene="ftsE"
                     /locus_tag="PKN55_11685"
     CDS             complement(3147..3800)
                     /gene="ftsE"
                     /locus_tag="PKN55_11685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HNX71950.1"
                     /translation="MIQFDNVTKRYPGGIDALKNLSFKVEKGEMVFLAGHSGAGKSTL
                     LRLVAGIDRATSGTVLVNNQNLGKLGGTQLSFMRQHIGLVFQDHKILFDRSVFDNVML
                     PLDIIGFEPREAAKRVRAALDKVGLLDREKSNPIRLSGGEQQRLCIARAVVHRPSILL
                     ADEPTGNLDRDYAHDIMELFRSFNQVGVTVLVSAHDEMLMHTYGRRIIRLAQGHLTP"
     gene            complement(3819..>4217)
                     /locus_tag="PKN55_11690"
     CDS             complement(3819..>4217)
                     /locus_tag="PKN55_11690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HNX71951.1"
                     /translation="VCYDAIQAAVARKIDIVLADTAGRLPTQLHLMEEIRKVKRVIDK
                     ALPGAPHEVILVLDANIGQNALTQVKAFDDALGLTGLILTKLDGTAKGGVIAAIAREH
                     PVPLRFIGVGEGLDDLRPFLARDYIDALFD"
BASE COUNT          762 a         1185 c         1364 g          906 t
ORIGIN      
        1 tgccaaagtt caagcaaggg gaggattatg cgcaggtccg cagtaagttg ctgaatgccg
       61 gctggaagcc acggcgtatg ccagatgccg atcaatgcat gcagggagat gatcgttgcc
      121 agggtcggcc cgagatggaa gcttgtgcag gaagtggtcg agccaattgc cgcttcgcct
      181 ggaagcgggc tggggcgctt ctgaccattt ttacggtagg ttcgcccgcc gtgtttgagg
      241 ctgccagcaa tgagtagctt ttgaacgaga acgccccttg ctcatttcat gggtaggggg
      301 cttcttgctg tttactcgcg tacttttcga accacttcaa aaggaaggat gttttccgca
      361 caggccgaga ataatgcgcc aaagagcctt tggtcgtctg tgagtatggt gtgatcagca
      421 gccagtctca ccagtgagct atcggtgagg ccatacgcca gaaatgtgtc ttgggcgcaa
      481 tctgaagtaa cgttggaaga atctgtgtga attgcgagaa caggattctg gccgtttcat
      541 aggcaagtct ggatgcgtag ccggtcaggt cgatcaagtt ggacacttcg gtggcaatgt
      601 agtgggtgat gcatatttct tggtggctgg ccatcacatc aagaatggca tcgtagtctt
      661 ggagggtgaa tttattcagg cgctttgagt tttgtatatg ccggccacct tctacggcgc
      721 caatgacaag aagaaggaga agatttgtgt cgatgatcaa cttcttcttg cccaagacat
      781 catccctcag tgctgcgctt gaaacctctg aactgtccgc tgtggctgtc cacaaagaag
      841 actttgcatt ctctgcgctt tttcatgatg gcaatcagcg ccatcaaatt ggcggcgttg
      901 tcttttgcgc tgtctggggc cggaggggga tcgtctcctt caagctcata gctgatggtg
      961 acttcataac gcgtgttgtc tgcagagatc agggcacctt ccagcaccag gtttttggcg
     1021 cccttcacca gctttacgac attctcttgg gcgatgcgta tcgcctcatc gaagtcaatc
     1081 atggcgatct ccgctgtgtt gccgctacgc caccaccagc gcctgcttca tcttctgcag
     1141 cgccttggct tcaatctggc gaatccgttc cgccgacacg ccgtattccg ctgccagatc
     1201 gtgcagggtc aggccgccgt cgtcggccag ccagcgcgat tcgacaatgc ggcggctgcg
     1261 ggcgtccagc gtggacagcg cgtgttccag gccttcgcgt tgcaggcggt cgaaggcttt
     1321 tttctcgatc tggcgggtgg gttcgttttc ggcgtcggcc agccagtcaa tcggcgcgta
     1381 gccgccttcg tcgtcgttgt cgtccgccat cagcgccacg tcctggccgc tcatgcgggt
     1441 ttccatttcg cgcacttctt ctggcttgac gccaaggtct tcggcgatct ggcgggcttc
     1501 tttgtcgctc agcgcggcaa agccggattt catcgaacgc aggttgaaaa acagcttgcg
     1561 ctgcggcttg gtggtggcca cgcgcaccag acgccagttg cgcaggatga attcgtggat
     1621 ttccgccttg atccagtgga tggcgaacga aaacagccgc acgccgcgct gcggctcaaa
     1681 acgcttcacg gctttcatca ggccgatatt gccttcctga atcaggtcgg cttgttgcag
     1741 gccgtagcca gaataaccgc gagcgacgga caccaccacg cgcaggtgcg acatcaccaa
     1801 acgccgggcg gcgtccaggt cgttgtgctg gtgcaggcgg gtggccagtt cggtttcttc
     1861 ctcctggctt agcatcggca cgctgttgac agtctggatg tactgttcaa ggctgccggc
     1921 ggcagccggg atgggcaagg caaacgtagc gctcataagg tggacaagct cctgtgttca
     1981 gccaatctta gcactcgaag tctgagagtg ccaaagtgtc gaacgagttc cggcaaaaaa
     2041 tttttcaatg gtgttgatag cctgccacta tgcctcaatc agacggccct gggtgggatg
     2101 acttttgtca gcgtcagcgg gtcgtgtggc ggaaaacccc ttggtcggca agggtatggc
     2161 cgctggcaga gctggctggc tgcgccgtcc ggggtcggac ggcgcagtgc ccggatgggt
     2221 ggcgaacaag acccggcaga ttcaggatgg ttccatctca cgcagtacat ggccggtggc
     2281 caggcgggcg cccagtgcgg ccagggtggc cacccccagc agcagggcgg cgacttcttc
     2341 tggcgccagc tgacgcaggg tggcgtggtt gttgtagagc gcggcgagct cggccacggc
     2401 cgggttggcg gtggcaatca gccaggcgct caatccccag gccaccagtg cggccagcac
     2461 gccctggaat acggcaaaat acatgaacgg ccggcggata aagccatcgg tggcgccaat
     2521 cagtttggag acttcgattt cattgcgccg ggccagaatc tgcaggcgga tggtgttgga
     2581 gctggccagc accagggcaa tgcccaaggc cgcccccagc aggatggtca actggtggcc
     2641 cagggtgagc aagctgtgca gcttgcgcgc ccagccggcg tcaaattggc tggtttccac
     2701 catcggcagg ccggacagtt ctttttgcag ccgctccagt gcttcgggtt cggtgtcctt
     2761 ggggctgacg ataaacgcat cgggcagcgg gttgccggcc aggccgtcgg tgacttcggc
     2821 gaggccattg cgcgccatca ggtctttcag cgcgctttcc ttgctgacaa agcggtgcga
     2881 gtgcaccagt gcgtgctgct tgagcgtctg gttgacggtt tccacgtcgg cccggtcggc
     2941 gttgagttcc atgaacaggg taatctgcgg cgaggcggcc aggccattgc tccagttggc
     3001 caggctggcc acggtcagat aaagccccag cggcaggctg atggccacgg ccagcatgaa
     3061 caggctgagc aggctgccca gcggctggcg caacagggtg cgtagcgcct ggcgggcggc
     3121 gcggccgtgc aggtagagca gatgtttcat ggggtcagat gtccctgtgc aagccggatg
     3181 atgcgtcggc cataggtgtg catcagcatt tcatcgtggg cggacaccag cacggtgacg
     3241 cccacctgat tgaatgagcg gaacagctcc atgatgtcat gggcgtagtc acggtccaga
     3301 ttgccggtcg gttcgtcggc cagcaggatg gacgggcggt gcaccaccgc gcgggcgatg
     3361 cacaggcgct gttgttcgcc gccggacaga cggatggggt tggatttttc ccggtcgagc
     3421 aggccgactt tgtccagcgc ggcgcgcacg cgcttggcgg cctcgcgcgg ctcgaagccg
     3481 atgatgtcca gcggcagcat gacattgtcg aataccgagc ggtcaaacag gattttatgg
     3541 tcctgaaaca ccaggccgat gtgctggcgc atgaacgaca gttgcgtgcc gcccagcttg
     3601 cccagattct ggttattgac cagcactgtg ccgctggtgg cgcggtcaat gccggccacc
     3661 agccgcagca gggtggattt gccggcgccg gagtggccgg ccagaaacac catttcgcct
     3721 ttttctacct tgaacgacag gtttttcaag gcgtcgatgc cgcctggata gcgtttggtg
     3781 acgttgtcga attggatcat ggtcatgtcc gcgccggctc agtcaaacag ggcgtcgata
     3841 tagtcgcgcg ccaggaaggg gcgcagatcg tccagccctt cgcccacgcc gataaagcgc
     3901 agcggcaccg ggtgctcgcg ggcgatggcg gcaatcaccc cgcccttggc ggtgccgtcc
     3961 agcttggtga ggatcaggcc ggtgaggccc agcgcgtcgt caaaggcttt tacctgggtc
     4021 agcgcgttct ggccgatatt ggcgtccagc accagaatca cctcgtgcgg cgcgccaggc
     4081 agcgccttgt cgatcacccg tttgaccttg cggatttctt ccatcaggtg cagttgggtg
     4141 ggcaggcggc cggcggtgtc ggccagcacg atgtcgatct tgcgcgccac tgccgcctgg
     4201 atggcgtcgt agcacac
//
LOCUS       DAOPYX010000261         4200 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_27565_length_4200_cov_8.969119, whole genome shotgun sequence.
ACCESSION   DAOPYX010000261 DAOPYX010000000
VERSION     DAOPYX010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 4200)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4200)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4200
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27565_length_4200_cov_8.969119"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..458)
                     /locus_tag="PKN55_11695"
     CDS             complement(<1..458)
                     /locus_tag="PKN55_11695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 family protein"
                     /protein_id="HNX71952.1"
                     /translation="MAGASLLALLDDIATLLDDVAVMTKVAAKKTAGVLGDDLALNAE
                     QVSGVRAERELPVVWAVAVGSLKNKAILVPAALAISALAPWLITPLLMLGGLYLCFEG
                     VEKLAHSWLHGAEEDANEHARQLAAVADEQVDLVAMEKDKIQGAIRTDFVL"
     gene            complement(539..2134)
                     /locus_tag="PKN55_11700"
     CDS             complement(539..2134)
                     /locus_tag="PKN55_11700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012697771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HNX71953.1"
                     /translation="MSAIPDPIAAGLARGWQVVDASTLRADTHYEADVVIIGTGAGGG
                     VSAEVLSQAGLKVLLIEEGPLPLGRDFRLLESDAYPQLYQACAARQTQDKAITILQGR
                     AVGGSTTVNWTSSFRTPPGTLAVWHERYDLPLTEAEMRPWFEQIETRLSIHDWPEAPN
                     ANNAVLARGAQQLGISHGRIRRNVKGCWNLGYCGMGCPTHAKQSMLVTTIPAALDAGA
                     RLLTRCRAWRLNLTAGEVSGLECQALDSAGLRANGVRITVRARHYVLAGGAINTPALL
                     LRSRLPDASGRLGKRTFLHPTVLSAALFAERVDAWHGAPQTVYSDHFLHTQDIDGPLG
                     YKLETPPVHPLLASVTLTGLGESHAALMRQMPHLHVMLALLRDGFHVDSQGGEVSLDD
                     DGHARLAYPLNDPLWEAARRAWLSMAELQFAAGARQVLPIHEDAVPYRSWPDAREAIR
                     QLPLEILRARWVSAHVMGGAMMGKDASRGVVNADGACWGMRNLSVLDGSVFPTSIGAN
                     PQLSVYALALRNATKLAQTLGHG"
     gene            complement(2131..2721)
                     /locus_tag="PKN55_11705"
     CDS             complement(2131..2721)
                     /locus_tag="PKN55_11705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX71954.1"
                     /translation="MHTAKKSTPLLTRRQFLRVGLAGGAVLLTARLVYGPFARMRLAE
                     VPEAEQLKTLNPRTATALAAIAPVMLGSAFPPAEPEETRLGAQHALVRAIDAAIAAMP
                     APVQAEISQLLDLLIFPPTRRLLVGLREEWARAEQDDIRGFLYRWRESRFQLLRAGYQ
                     GLHQLVCAGWYAMPASQAAVGYPGPPVQWKLAEGAA"
     gene            complement(2755..>4200)
                     /locus_tag="PKN55_11710"
     CDS             complement(2755..>4200)
                     /locus_tag="PKN55_11710"
                     /EC_number="1.2.1.68"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050269 - coniferyl-aldehyde dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006081 - cellular aldehyde metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coniferyl aldehyde dehydrogenase"
                     /protein_id="HNX71955.1"
                     /translation="KGAYHMPSAPLLTTPAELNHALSTARAAWQAAPYPSCAERRARL
                     AALRAALQGHAGALAAAVSADFGQRSADETRMAELFPTLLAIDHARRHLRRWMRPQRR
                     GVSPWFWPARAQVLPQPLGVVGVIVPWNYPLYLALGPLVDALAAGNRVLIKMSEYTPR
                     TAAAVATLLAEALPGGEAQVALGEVALAQTFASLPFDHLLFTGSTAVGREVMRLASAQ
                     LTPVTLELGGKSPVLIAPGHDVARAAACIAQGKLLNAGQTCVAPDYVLLPRGARAEFV
                     RAFAQAVAASYPTLADNPDYTSIIHPRHWQRLHDGLAEARAAGAQTHACVGPDSEAAL
                     AAARKIAPHLLWDVPADCQLLREEIFGPILPLVEYEDWPAALAYVNARPRPLALYLFD
                     HDRQRVQAVLRQTIAGGVVVNDTLLHVVQDGLPFGGVGASGMGQYHGHDGFLTFSKLK
                     PVLYQSRWNAMPWLRPPYGRLARWLQALMLR"
BASE COUNT          722 a         1454 c         1397 g          627 t
ORIGIN      
        1 agcacaaaat ccgtgcgaat ggctccctgg attttgtctt tttccatggc caccagatcc
       61 acctgctcat cggccaccgc cgccaactgg cgggcatgct cgttggcgtc ttcttcggcg
      121 ccgtgcagcc atgagtgcgc cagtttttcc acaccctcaa aacacagata cagcccaccc
      181 agcatcagca aaggggtaat cagccacggc gccagcgcgc tgatggccag tgcggccggc
      241 accagaatcg ccttgttttt caacgagccc accgccactg cccacaccac cggcaattcg
      301 cgctcggcgc gcaccccact gacttgctcg gcattcagcg ccagatcatc gcccagcacc
      361 ccggcggttt tcttggcggc gactttggtc atcaccgcca cgtcgtccag caaggtggcg
      421 atatcgtcga gcaaggcaag caaactggcc ccggccatgg gtattcctca aaaataaaaa
      481 tggaacggca tgatagcgca ggcaggccgg attggggttg ggatggcgcg tgtgggtatt
      541 atccgtggcc cagggtttgc gccagttttg tcgcattgcg cagcgccagc gcatacaccg
      601 acaactgcgg gttggcgcca atgctggtgg gaaacaccga gccatccagc accgacagat
      661 tgcgcatgcc ccagcaagcg ccgtcggcgt tgaccacgcc acggctggcg tccttgccca
      721 tcatcgcccc gcccatcaca tgcgcgctca cccagcgcgc gcgcaggatt tccagcggca
      781 gctggcgaat cgcctcgcgc gcgtctggcc aactgcggta gggcacggcg tcttcgtgaa
      841 tcggcagcac ctggcgcgcg ccagcggcaa attgcagctc ggccatgctc agccaggcgc
      901 gccgcgccgc ttcccacagc gggtcgttca gcggataggc cagccgggcg tggccgtcgt
      961 cgtccaggct gacttcgccg ccctggctat ccacatgaaa accatcgcgc aatagcgcca
     1021 gcatcacatg caggtgcggc atttgccgca tcagcgcggc gtggctttcg cccaggccgg
     1081 tcagcgtcac cgaggccagc agcgggtgca cgggcggggt ttccagcttg tagcccagcg
     1141 ggccgtcgat gtcctgcgtg tgcaaaaaat gatcgctgta cacggtttgc ggcgcgccat
     1201 gccaggcgtc aacgcgctcg gcaaacagcg cggcggacag cacggtgggg tgcaaaaagg
     1261 tgcgcttgcc cagccggcca gaggcatccg gcaggcggct gcgcagcaag agcgccgggg
     1321 tgttgatggc gccgccggcc agcacatagt gacgggcgcg caccgtgatg cgcacgccat
     1381 tggcgcgcag gccggcgctg tccagcgcct ggcattccag cccgctcacc tcgccggcgg
     1441 tcaggttcag ccgccaggcg cggcagcggg tcagcagccg cgcgccagcg tccagcgcgg
     1501 cgggaatggt ggtcaccagc atggattgct tggcgtgggt ggggcagccc atgccgcagt
     1561 agcccagatt ccagcagcct ttgacattgc ggcggatgcg gccgtggctg atgcccagct
     1621 gctgggcgcc gcgcgccagc acggcgttat tggcgttggg cgcttctggc cagtcgtgaa
     1681 tcgacaggcg ggtttcgatc tgctcaaacc acgggcgcat ctcggcctcg gtcagcggca
     1741 ggtcgtagcg ctcgtgccac accgccagcg tgcccggcgg cgtgcggaat gacgacgtcc
     1801 agttcaccgt ggtcgagccg cccaccgcgc ggccctgcaa gatggtgatg gccttatcct
     1861 gggtttgccg cgccgcgcag gcctgataca gctgcgggta ggcgtcgctt tccagcagac
     1921 gaaaatcccg gcccagcggc agcgggcctt cttcgatcag cagcactttc agcccggcct
     1981 ggctcaacac ctcggcgctg accccaccgc cagcgccggt gccaataatc accacatcgg
     2041 cttcatagtg ggtgtcggcg cgcagggtgg acgcatccac cacctgccag ccacgcgcca
     2101 gaccagcggc aatcggatcg ggaatggcgc tcatgctgcg ccctccgcca gtttccattg
     2161 cactggcggg ccggggtagc ccaccgccgc ctggctggcc ggcatggcgt accagccggc
     2221 gcacaccagc tgatgcaggc cctgatagcc ggcgcgcagc agctgaaagc ggctttcccg
     2281 ccagcgatac aaaaagccgc gaatatcgtc ctgctcggcg cgcgcccact cctcacgcag
     2341 gcccaccagc aaacgccggg tgggcggaaa aatcaataaa tccagcaatt ggctgatttc
     2401 tgcctgcacc ggcgccggca tggcggcaat cgcggcgtcg atggcgcgca ccagcgcatg
     2461 ctgcgcgccc aggcgcgttt cttccggctc ggccggcgga aacgccgagc ccagcatcac
     2521 cggggcgatg gccgccagcg cggtggcggt gcgcgggttc agggttttca gctgctcggc
     2581 ctcgggcacc tcggccagcc gcatgcgggc aaacgggccg tacaccagcc gcgcggtcag
     2641 cagcacggcg ccgccggcca ggccgacgcg cagaaactgg cggcgggtca acaagggggt
     2701 agattttttt gcagtgtgca tgggtgatga gcctgaatca aaaaccgggg atgctcaacg
     2761 cagcatcagc gcttgcagcc agcgcgccag ccggccatac ggcgggcgca gccagggcat
     2821 ggcgttccag cgcgattggt aaagcaccgg cttgagcttg ctgaacgtca gaaagccgtc
     2881 atggccatga tactgcccca tgccagatgc gcccaccccg ccaaacggca gcccgtcctg
     2941 caccacatgc aataaggtgt cgttgaccac cacgccgcca gcgatggtct ggcgcaaaac
     3001 agcctgcacc cgctggcggt catggtcaaa cagatacaag gccagcgggc gcggccgggc
     3061 gttgacatag gccagcgcgg ccggccagtc ctcgtattcc accagcggca aaatcgggcc
     3121 aaagatttct tcgcgcagca gttggcagtc ggcggggacg tcccacagca gatgcggggc
     3181 aatcttgcgc gcggcggcca gcgccgcctc gctgtccggg ccgacacagg cgtgcgtttg
     3241 cgcgccagcg gcgcgcgcct cggccaggcc gtcgtgcagg cgctgccagt ggcgcggatg
     3301 aatgatgctg gtgtaatccg ggttgtcggc cagcgtcggg tagctggcgg ccaccgcctg
     3361 ggcaaaggcg cggacaaact cggcgcgcgc gccgcgcggc agcagcacat aatccggcgc
     3421 cacgcaggtt tgcccggcgt tcagcagctt gccctgggca atgcacgccg ccgcgcgcgc
     3481 cacatcgtgg ccgggggcaa tcaagaccgg cgatttgccg cccagctcca gcgtcaccgg
     3541 ggtgagctgg gcgctggcca gacgcatcac ctcgcgcccc acggcggtag agccggtaaa
     3601 cagcaggtgg tcaaacggca gactggcaaa ggtctgcgcc agcgccacct cgcccaacgc
     3661 cacctgcgcc tcgccgcccg gcaaggcctc ggccagcagc gtggccacgg cggcggcggt
     3721 gcgcggggtg tattcgctca tcttgatcaa cacccggttg cccgccgcca gcgcatccac
     3781 cagcgggccc aaggccagat acagcgggta attccacggc acgatcaccc cgaccacccc
     3841 caagggctgc ggcaacacct gagcgcgcgc cggccaaaac cacgggctga cgccgcgccg
     3901 ctgcgggcgc atccagcggc gcagatgccg gcgggcgtgg tcgatggcca gcagggtagg
     3961 gaacagctcg gccatgcgcg tttcgtcggc gctgcgctgg ccaaaatcgg ccgacaccgc
     4021 cgccgccagc gcgccggcat ggccttgcaa ggccgcgcgc agggccgcca gccgggcgcg
     4081 gcgctcggcg caggacggat aaggcgccgc ctgccaggcg gcgcgggccg tgctcagggc
     4141 gtggttcagc tcggccgggg tggtgagcag tggggcgctg ggcatatggt atgctccttt
//
LOCUS       DAOPYX010000262         4195 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_27620_length_4195_cov_3.811111, whole genome shotgun sequence.
ACCESSION   DAOPYX010000262 DAOPYX010000000
VERSION     DAOPYX010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 4195)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4195)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4195
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27620_length_4195_cov_3.811111"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1001)
                     /locus_tag="PKN55_11715"
     CDS             complement(<1..1001)
                     /locus_tag="PKN55_11715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012698257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing methyl-accepting
                     chemotaxis protein"
                     /protein_id="HNX71956.1"
                     /translation="MKLNLPVTQHEKPFTHGAIVTKTNLKGVITYANDAFIEISGFER
                     DELIGASQNILRHPDVPPCIFEDMWLTLKCGDTWKGVVKNRCKNGDHYWVSAFIVPVK
                     QDGQTTGYMSVRTPASRADIRTAEALYARLGRDGKLPRQRRLPTLTDAKVNLAAIAVF
                     NLLLLTLAVQHGQWSDWLALALGLSVSLGLLARGWSSSQRMGRIARMLDHIAEGRLNN
                     SIAIDRRDEIGQIEAGLATMQVHIKVMIDDLTQAATLMHSHSHGLNQLMRQLLERFAQ
                     QSHEVNGVSSAVEQMSISVSQVAEHAGSAASAAGQARAVAGAGANQMAQSCEETRAAA
                     "
     gene            complement(1424..2743)
                     /locus_tag="PKN55_11720"
     CDS             complement(1424..2743)
                     /locus_tag="PKN55_11720"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing methyl-accepting
                     chemotaxis protein"
                     /protein_id="HNX71957.1"
                     /translation="MFNTRLKQAHVALQQQLTEYQAERTALARSMAMVRFDRDGKVIE
                     ANPQFLSTLGYAQASEVLGKPHSQFCLREETASPAYQQFWDRLRRGEPFCGRVRRQAA
                     DGHVVWLEATYAPVLDAQGAVESYLKLAADITPQVEAEASQRARLNAINRAMAVIEFT
                     PDGTVVDANDNFLATLGYRLEDIKGRHHRMFCDSQFAQSAEYQHHWQQLARGEFFTGQ
                     IKRIAKDGSVRWLEASYNPVFGTDGKTVTSVIKFASDITARVQAQRKESESARFAYCV
                     AQETAELSHTGSDTVRRNVGAIEDMAGSIEEAGRNVQALGERSEQITSIVQTIKDIAD
                     QTNLLALNAAIEAARAGETGRGFAVVADEVRKLAERTAASTAEISKMVSDIQQQTTQA
                     VDNMSAIQQQANHSVTLTRESGETLAQILEGARSVVKAISQYAERQP"
     gene            2983..3315
                     /locus_tag="PKN55_11725"
     CDS             2983..3315
                     /locus_tag="PKN55_11725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNX71958.1"
                     /translation="MSAYLKRSLIPRPLSSLRDVLAYNLRVSRLARGLSQEQLGFAAQ
                     LDRTFISQVERARVNISLDNLDKLARALALDAHELLHPPFSAWTPRQLERPSKQNAVN
                     LAADGVEA"
     gene            complement(3337..>4195)
                     /locus_tag="PKN55_11730"
     CDS             complement(3337..>4195)
                     /locus_tag="PKN55_11730"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNX71959.1"
                     /translation="VMIDDLSAAAGLMHANSQGLHRVMTQLMERFAQQSHEVADISGA
                     VEEMSVSVSQVAEHASGAASAASQATGVAQTGAQHMAQSRQETQVAAQSVSHAQATIQ
                     DLYESVVKISTVTDTIREIADQTNLLALNAAIEAARAGESGRGFAVVADEVRRLAERT
                     GHSTAEINQIVANIRVVTDSAVDNMRKVSDSSEQSERHLADTANSLNDILQASQSVEQ
                     MMRSIADTNVQQSATAHELAERTVGISTRIESSTQEIGQAGRLIAELAGKAEDLQAMI
                     RRFDTGDKR"
BASE COUNT          742 a         1225 c         1302 g          926 t
ORIGIN      
        1 gccgccgcgc gggtttcctc gcaggattgc gccatctggt tggcgccagc gccagccacg
       61 gcgcgcgcct ggccggctgc gctggcggcg ctgccggcgt gttcggccac ttgtgacacg
      121 gaaatactca tctgctccac cgcgctggaa accccgttga cctcgtgcga ttgctgggca
      181 aagcgctcca gcaactggcg catcagctgg ttcagcccgt ggctgtgcga atgcatcagt
      241 gtggcggcct gggttaaatc atcaatcatc accttgatat gcacctgcat ggtggccaac
      301 ccggcttcga tctggccgat ttcatcgcgg cggtcgatgg cgatgctgtt gttcagccgc
      361 ccttcggcga tatggtccag catgcgggca atccgcccca tgcgttggct ggaggaccag
      421 ccgcgcgcca gcaagcccag cgacacgctc aggcccagcg ccagcgccag ccagtcgctc
      481 cactggccat gctgaaccgc cagcgtcagc aacagcaggt tgaacaccgc gatggcggcc
      541 aggttcacct tggcgtcggt cagcgtcggc aggcgacgct ggcgcggcaa cttgccgtcg
      601 cggcccagcc tggcgtacag cgcctcggcc gtgcggatgt cggcgcggct ggcgggggtg
      661 cgcaccgaca tatagccggt ggtctggcca tcctgtttga ccggcacgat aaaagctgac
      721 acccagtagt ggtcgccatt tttacagcgg tttttcacca cgcctttcca ggtatcgccg
      781 catttgagcg tgagccacat gtcttcgaaa atacagggcg gcacgtccgg gtggcgcaag
      841 atattctggc tggcgccaat cagttcgtcg cgctcaaagc cggatatctc gataaaggcg
      901 tcattggcgt aagtgatcac ccccttgagg ttggtcttgg tgacgatggc gccgtgggta
      961 aacggctttt catgctgggt gacgggcaga ttgagtttca tgtctggtct ctctcacgaa
     1021 atcatcagtc agtggctgat ggcggggtgg ggatgaacca aacctgacac tttgtatgct
     1081 aaacgacagg ccaacggcgt gttggcgtgc cttggcactc aaggccagga ccgtgccggg
     1141 cgcggccagt ttccgggggg atgacgggga tgcgcacggt gtcggccggg tggcatggtg
     1201 ggcacagcgc cacacctgca aacggcttgc tggtatttca gcaattttct tgcctgcccg
     1261 gcaaaatggc gtcgccatgc tccgctggcc tgtctggaca ggctttcagc cggcgttgta
     1321 tagcgtaata tattgaatga aaaagagatt gtgcatcagc aggatgcgcc ggcatggggc
     1381 gcaagcctgc tgatggtgcg ttgcagcatc gtgaagagaa gggtcagggc tggcgttctg
     1441 cgtactgact gatggctttc accaccgagc gggcaccttc cagaatctgc gccagagttt
     1501 cgccagattc gcgggtcagc gtcacgctgt ggttggcctg ctgctggatg gcgctcatat
     1561 tgtccacggc ctgagtggtc tgctgctgga tgtcggacac cattttgctg atttcggcgg
     1621 tagaggcggc ggtgcgctcg gccagcttgc gcacttcgtc ggccaccacg gcaaagcccc
     1681 tgccggtttc gccagcgcgg gcagcttcaa tggcggcgtt cagggccagc agattggtct
     1741 ggtcggcaat gtctttgatg gtctgcacaa tcgaggtgat ctgctccgag cgctcgccca
     1801 gcgcctgtac attgcgcccg gcttcttcga tgctgccagc catgtcttca atggcgccga
     1861 cattgcggcg cacggtgtct gagccggtgt gcgacagctc ggcggtttcc tgtgccacgc
     1921 aataggcaaa gcgcgcgctt tcgctttcct tgcgttgtgc ttgcacgcgg gcggtgatgt
     1981 ccgaggcaaa tttgatcacc gaagtgacgg ttttgccatc agtgccaaac accgggttat
     2041 agctggcttc cagccagcgt accgagccat ctttggcaat gcgtttgatc tggccagtga
     2101 aaaattcccc acgtgccagt tgttgccagt ggtgttgata ttctgccgac tgggcaaact
     2161 ggctgtcgca gaacatgcgg tgatggcggc ccttgatatc ctccaggcga taacccaagg
     2221 tggccagaaa attgtcgttg gcgtccacca cggtgccatc cggggtaaat tcaatgacgg
     2281 ccatggcgcg attaatggcg ttcagtcgtg cgcgttggct ggcttcggct tcgacttgag
     2341 gggtaatgtc ggcggccagt ttgagataag actccactgc gccttgcgca tccagcaccg
     2401 gtgcataggt ggcttccagc cataccacat gcccatccgc tgcctgacgc cgcactcggc
     2461 cgcaaaacgg ttcgcctcgg cgcagacgat cccagaattg ctggtacgcc gggctggccg
     2521 tctcttcgcg caggcagaac tggctgtgtg gcttgcccag cacctcgctg gcctgagcat
     2581 agcccagcgt ggacaaaaac tgcgggtttg cctcaatcac cttgccgtcg cgatcaaagc
     2641 gcaccatggc catcgaacgg gccaacgccg tgcgttccgc ctggtattca gtcaattgtt
     2701 gttgcagggc tacgtgggct tgcttcagac gggtattgaa catcacgggc tctctcaagc
     2761 ggtcgatccg cggcaggaag gaaagaagaa aggctggata cgctcttgcg tgtatgacag
     2821 gaatggcgcc agaaaggcga tatctatatg ccttgtttgc agattttaac acatgcgaat
     2881 gtgggcttat acggggttta catcacgtct actggacgga aaatcacaaa atgtgatgta
     2941 aaagccgtag aagatggcct gtttcatgtt gacgatgttg gcatgtctgc ctacttgaaa
     3001 cgctctctga ttccacgtcc cttgtcctcc ttgcgcgatg tgctggctta caacctgcgc
     3061 gtcagccggc tggcgcgcgg cttgtcgcaa gagcagttgg ggtttgccgc acaacttgac
     3121 cggactttca tcagtcaggt tgagcgtgcg cgggtcaata tctcgctgga caatctggac
     3181 aaactggcgc gcgcattggc actggatgcc catgaattgc tgcatccacc attcagcgcc
     3241 tggacgccac gccaattgga gcggcctagc aagcaaaacg ccgtcaacct ggcggctgac
     3301 ggcgtagaag cgtgagatcg atcgtgcgtg acaggctcag cgtttatccc cggtgtcaaa
     3361 gcggcggatc attgcctgca aatcctccgc cttgccggcc agttcggcaa tcaggcgccc
     3421 ggcctggccg atttcctgag tggatgattc aatgcgggtg ctgatgccca cggtgcgttc
     3481 ggccaattca tgggcggtgg cagactgctg cacattggtg tcggcgatgc tgcgcatcat
     3541 ctgctccacc gactggctgg cttgcagaat atcgttcagg ctattggccg tgtctgccag
     3601 atgacgctcg ctttgttccg aactgtcact gaccttgcgc atattgtcca ctgccgaatc
     3661 cgtcacgacc cggatgttgg ccacgatctg gttgatttct gctgtgctgt ggccggtgcg
     3721 ctcggccaac cgacgcacct cgtcggccac cacggcaaag ccacggccgg attcgccagc
     3781 gcgggcggcc tcaatggcgg cgttcagcgc caggaggttg gtctgatcgg caatctcgcg
     3841 aatggtgtcg gtcactgtgc tgattttcac cacagattca tacaaatcct ggatcgtggc
     3901 ttgggcgtgg ctgacgcttt gcgcggccac ctgggtttct tggcgcgatt gcgccatgtg
     3961 ctgtgcgccg gtctgcgcca cccccgtggc ctggctggct gcactggcgg cgccgctggc
     4021 gtgttcggcc acctggctga ccgagacgct catttcttct actgcgccag agatgtcggc
     4081 cacttcgtgc gattgctggg caaagcgctc catcaactgt gtcatcacgc gatgcaggcc
     4141 ttggctgttg gcgtgcatca aaccggcggc ggcggacaaa tcgtcaatca tcacc
//
LOCUS       DAOPYX010000263         4174 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_27797_length_4174_cov_9.130371, whole genome shotgun sequence.
ACCESSION   DAOPYX010000263 DAOPYX010000000
VERSION     DAOPYX010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 4174)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4174)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4174
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27797_length_4174_cov_9.130371"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1163)
                     /locus_tag="PKN55_11735"
     CDS             complement(<1..1163)
                     /locus_tag="PKN55_11735"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNX71960.1"
                     /translation="MSRFFINRPIFASVISIIIVIAGLVALQVLPIAQYPQIAPPTVV
                     ITATYPGASAETLSKTVAAPIEEQLNGVENMLYFTSSAAANGVVSITATFEVGTNADM
                     AAVNVNNRVKAAEPRLPETVRRNGVVVQKRSTDILQVVALKSDGGKYSTLFLSNYASL
                     NIVDELKRVKGVGDVTVFGAQDYSMRIWLKPDRMAQLGVTTSEIAAAINAQNAQNAAG
                     KIGQEPAPNDQQLVYTVTAKGRLLSEDEFGNIVIRASGPSGVLRLKDVARIELGAYSY
                     EQRVTLDGQPTIAMGVFLQTGANALEVADLVQAKMQELQKKFPQGMGYVVPVDSTRFV
                     TASIKEVKSTLIEAMVLVIAVVFLFLQSWRATLIPMVAVPISLIGTFAGLWLFG"
     gene            complement(1167..2309)
                     /locus_tag="PKN55_11740"
     CDS             complement(1167..2309)
                     /locus_tag="PKN55_11740"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNX71961.1"
                     /translation="MTLAKRQFFSSPSLSAWLTMACVGVVLAGCGEKKADAPPPQGPM
                     PVTVLDMQPQKVPTRVEVMGQAEGAREAEVRARVGGILLKRLYQEGEPVKAGQALFQI
                     DRAPYEIAAAEARARAEQTAREVARLKGLAEAKAVAQKDYDDAVSANAMAQATLKQAE
                     LNLSWTTVTAPVAGVAGRAVKSEGNLISTADSLLTNVQQVNPAWVRFSLGEGDLAKFP
                     ARRVTAEAVQAVELVLPDGSVYPKRGKLNFVASNIDTTLGTQQLRAEFDNSEQLILPG
                     QFVRVRLVTGERDGVFLVPQTAVAQGEQGARVMTVGADNKATPRPVQAGEWLGKDWVI
                     LGGLKAGDKVIIDNLIKLKPGMPVKPRAPGEAPAGGDKPAADGKKG"
     gene            2496..3005
                     /locus_tag="PKN55_11745"
     CDS             2496..3005
                     /locus_tag="PKN55_11745"
                     /inference="COORDINATES: protein motif:HMM:NF013231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HNX71962.1"
                     /translation="MNHMSHHGWDVSLDPHTVPVFLWLQWAHRRSLQPMQPTLARHAL
                     SSAEFDLLATLRNAEPDYTLTPSQLLEQMLLTSGGLSKVMAQLEARGLIARLCRNDDR
                     RIKPVRLTTDGQALIEQAMADAVAKAGAWLRTALSGEELTQLTGLLQRVAEADVPDEN
                     APTACSGAQ"
     gene            complement(3023..3553)
                     /locus_tag="PKN55_11750"
     CDS             complement(3023..3553)
                     /locus_tag="PKN55_11750"
                     /inference="COORDINATES: protein motif:HMM:NF014471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HNX71963.1"
                     /translation="MHDESLWLEIAWLCLLISLMAIGGANAMVPEIHRYVIEQRGWMR
                     GDEFAALFAIAQASPGPNVLFITLIGWKVAGWPGAVASTVAMCGPSSTLALAAGRTMA
                     RNPQSRWRRVIKSGLAPLTIGLILAAGWVLTRNTVHSWADAALCAVAVAASLHGRINP
                     VWLIGLGAAAGVVWGG"
     gene            complement(3555..4097)
                     /locus_tag="PKN55_11755"
     CDS             complement(3555..4097)
                     /locus_tag="PKN55_11755"
                     /inference="COORDINATES: protein motif:HMM:NF014471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HNX71964.1"
                     /translation="MTPLVTPTVWQLFTGFSAVALSGFGGVLPITRRTLVEKRRWLDE
                     AEFVELLSLGQLLPGPNIVNLAVMFGDRRRGLPGALAAVAGLLTGPLMVVLTLASLHQ
                     RYGHLSMVQHAISGMAAAACGLLVAMGVKMAWLHPKNPRTVAVALLAMAAIAGLGWPL
                     LKVLLIAGPMALLWAAREER"
BASE COUNT          761 a         1414 c         1290 g          709 t
ORIGIN      
        1 ccaaacagcc acagcccggc aaaagtgcca atcagcgaaa tcggcaccgc caccatcgga
       61 atcagcgtgg cgcgccagct ttgcaggaac aaaaacacca cagcaatcac cagcaccatg
      121 gcttcgatca gcgtgctttt cacctctttg atcgaggcgg tgacaaagcg ggtgctgtcc
      181 actggcacca catagcccat gccttgcgga aatttctttt gcagctcctg catcttggcc
      241 tgtaccaagt cggccacttc cagcgcattg gcgccggttt gcaggaacac gcccatggcg
      301 atggtcggct ggccgtccag cgtcaccctt tgctcgtagc tgtaagcgcc cagctcgatg
      361 cgcgccacat ctttcagccg cagcacgccg ctggggccgc tggcgcggat gacgatattg
      421 ccgaattcat cttcgctgag cagccggccc ttggcggtga cggtgtacac caactgctgg
      481 tcgttcggcg ccggctcctg gccaatcttg ccagcggcat tctgcgcgtt ctgggcgttg
      541 atggcggcgg cgatttcgct ggtggtcacc cccagctggg ccatgcggtc gggcttgagc
      601 cagatgcgca tggagtaatc ctgcgcgcca aacaccgtca catcgcccac gcccttcacc
      661 cgcttgagtt catcaacgat gttcagcgag gcgtagttgg acaaaaacag cgtgctgtat
      721 ttgccgccgt cggatttcag cgccaccact tgcaggatgt cggtcgagcg cttttgcacc
      781 accacgccat tgcgccgcac ggtttctggc agacgcggct cggcagcttt gacccggtta
      841 ttgacgttca ccgccgccat atcggcattg gtgcccacct cgaaggtggc ggtgatgctc
      901 accacgccgt tggccgccgc cgacgaggtg aaatacagca tgttctcgac gccgttgagc
      961 tgttcttcaa tcggcgcggc caccgtcttg gacagggttt ctgccgaggc gcccggatag
     1021 gtggcggtga tcaccacggt gggcggggcg atttgcgggt attgcgcaat cggcaatacc
     1081 tgcaaggcca ccagcccggc aatcacgatg atgatggaga taaccgaggc aaaaatcggc
     1141 cggttgataa aaaatctgga catggctcag cctttcttgc catcggcggc cggcttgtcg
     1201 ccgccagccg gcgcctcgcc cggcgcgcgc ggcttgaccg gcatgcccgg cttgagcttg
     1261 atcagattgt cgataatcac cttatcgccc gccttcagcc cgcccagaat cacccaatcc
     1321 ttgcccagcc attcgccagc ctgcaccggg cgcggcgtgg ctttattgtc tgcgcccacc
     1381 gtcatcaccc gcgcgccctg ctcgccttgc gccacggcgg tttgcggcac caggaacacc
     1441 ccgtcacgct cacccgtgac cagacgcacg cgcacaaact gacccggcag aatcaattgc
     1501 tcgctgttat cgaattcggc acgcagctgt tgtgtgccca gcgtggtgtc gatattgctg
     1561 gccacaaagt tcagcttgcc gcgcttgggg tagaccgagc catccggcaa caccagctcc
     1621 accgcctgca ccgcttcagc cgtcacccgc cgcgccggaa acttggccag atcgccctcg
     1681 cccagcgaaa agcgcaccca ggccggattc acctgctgca cattggtcag caggctgtcg
     1741 gcagtggaaa tcaaattgcc ttcggatttc accgcgcggc ccgccacccc ggccaccggc
     1801 gcggtgaccg tggtccacga cagattcagc tcggcctgct tgagcgttgc ctgtgccatg
     1861 gcattggccg ataccgcatc gtcgtagtct ttttgcgcca ccgccttggc ctcggccagc
     1921 cccttcagcc gcgccacttc gcgcgcggtc tgctcggcgc gcgcgcgcgc ctcggcggcg
     1981 gcaatttcat acggggcgcg gtcgatctgg aacagcgcct gcccggcttt caccggctcg
     2041 ccttcctgat acaggcgctt gagcagaatc ccacccaccc gcgcgcgcac ctcggcctca
     2101 cgcgcgccct cggcctgacc catcacctcg acgcgggtgg gcactttttg tggttgcatg
     2161 tccagcacgg tgaccggcat cggcccttgc ggcggcggcg cgtcggcttt tttctcgccg
     2221 caaccggcca acaccacgcc gacgcaggcc atggtcaacc aggcggacaa ggatggactg
     2281 gaaaaaaatt ggcgcttggc aagcgtcatg gtgggattcc tttgtctgga tcagggggcg
     2341 cgccggcggc ggacgcgccg gaattcagtt ggataaccaa gggggtaagg gtatgctggt
     2401 attatctttc cagcaagata cccggcaggg ttttacttta tgttacatgg ccgccgacac
     2461 cgtgtgccaa cgccagcgca gaaaggggca agacaatgaa ccatatgtca caccacggct
     2521 gggatgtctc gctagacccg cacaccgtgc cggtgttcct ctggctgcaa tgggcgcatc
     2581 gccgctcact gcagcccatg cagccgacac tggcccgaca tgcgctgtcc agcgccgaat
     2641 tcgacctgct ggccaccctg cgcaacgccg aaccggatta caccctcacc ccttcgcaat
     2701 tgctggaaca gatgctgctg acctcaggcg gcttgagcaa ggtgatggcg caactggaag
     2761 cgcgcgggct gattgcccgg ctatgccgca acgacgaccg ccgcatcaag ccggtgcggc
     2821 tgaccaccga cggccaggcg ctgatcgaac aagccatggc cgacgccgtg gccaaggccg
     2881 gcgcctggct gcgcacggcg ctgagtgggg aagaactgac ccaactgacg ggtttgttgc
     2941 agcgggtggc ggaggcagac gtaccggacg agaatgcgcc gacagcgtgc tcaggcgctc
     3001 agtgatatcc ctgccgtggc gctcaccctc cccacaccac ccccgccgcc gcgcccagcc
     3061 cgatcaacca caccgggttg atgcgcccgt gcagcgaggc ggccaccgcc acggcgcata
     3121 gcgcggcgtc ggcccagctg tgcacggtgt tgcgggtgag cacccagccg gcggccagaa
     3181 tcaggccgat ggtcagcggg gccaggccgg atttgatcac ccgccgccag cgcgattgcg
     3241 ggttgcgcgc catggtgcgc ccggcggcca gcgccagggt ggacgagggg ccgcacatgg
     3301 ccacggtgga ggccaccgcg ccgggccagc cggccacttt ccagccgatc agggtgatga
     3361 acagcacatt ggggccgggc gaggcttggg cgatggcgaa cagcgcggca aattcgtcgc
     3421 cgcgcatcca gccgcgctgc tcgatcacat agcggtgaat ctccggcacc atggcgttgg
     3481 cgccgccgat ggccatcagc gaaatcaata aacacagcca ggcgatttcc agccacaggc
     3541 tttcgtcgtg catgtcagcg ctcctcgcgc gcggcccaca gcagcgccat cgggccggca
     3601 atcagcagca ccttgagcag cggccagccc aggccggcga tggcggccat ggccagcagc
     3661 gccaccgcca cggtgcgcgg gttttttggg tgcagccagg ccattttgac gcccatggcc
     3721 accagcaggc cgcaggcggc ggcggccatg ccgctgatgg cgtgctgcac catcgacagg
     3781 tggccatagc gctggtgcag gctggccagg gtgagcacca ccatcagcgg cccggtcaac
     3841 aagccagcca ctgccgccaa cgcgccgggc aggccgcgac ggcggtcgcc aaacatcacc
     3901 gccagattga cgatattcgg gccgggcaag agctggccca ggctgagcag ctcgacaaac
     3961 tcggcttcgt ccagccagcg gcgtttttcc accagggtgc gccgggtaat cggcagcacc
     4021 ccgccaaaac cggacagggc gacggcagaa aacccggtga acagttgcca gacggtgggg
     4081 gtgacaagtg gggtcatgag ggatgttctc ctggcggcga caagggcgcc atcatcgcat
     4141 ggcgcgagag ggtgaacaaa aaacccgcaa aaaa
//
LOCUS       DAOPYX010000264         4162 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_27885_length_4162_cov_11.190894, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000264 DAOPYX010000000
VERSION     DAOPYX010000264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 4162)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4162)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4162
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27885_length_4162_cov_11.190894"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(104..1594)
                     /locus_tag="PKN55_11760"
     CDS             complement(104..1594)
                     /locus_tag="PKN55_11760"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNX71965.1"
                     /translation="MPAALWPRASLLRRSLFWKILLAFWLTLLLAGVGAGLVVWWHIP
                     KHEHQPLSGGPRTALLLHMAESVLVHGDETTLRRILIDWSGREAPIFAVDEQNRELLG
                     RKVEPEAIATARQLAAGKGDDARVRSVTLSSEHTYVLFVPGHEFRPRIGLLPFMPPLP
                     DGNFRRGDDGRPRDGHGGPPGFERGDKFRGGPGLPLPWVHVLAGILASLAFSAGLAWY
                     VAKPIGHLRQAFRAVSAGKLNTRVRARMGRRRDEFSDLAQDFDSMTGQLEALIGAQRR
                     LLHDISHELRSPLARLQAAIGLVRQNPQQLDATLDRLEREVTRLDELVDEVLTLARLD
                     SGLPGVSSQSFDLLALLDEVIGDAQFEAESLHKCVRYRGGGEAHMHGRADLLMRAVEN
                     IVRNAIRYTPESQCVEVESQCQGGQVRISVSDHGPGVADSELDAIFQPFYRSPSNPTG
                     TGYGLGLAIARRAVESHGGQIVARNRPEGGLRVEITLPLTPVAEPH"
     gene            complement(1597..2283)
                     /locus_tag="PKN55_11765"
     CDS             complement(1597..2283)
                     /locus_tag="PKN55_11765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008952403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNX71966.1"
                     /translation="MNRLLLVDDDVELAEMLRDYLAQEGFAVTLAHDGEAGAQEALSG
                     QYALIVLDVMMPRASGVEVLGRVRSQISTPIIMLTAKGDDVDRIIGLEMGADDYVPKP
                     CTPRELVARIRAVLRRVNSTPTESADNLLQIGELTLWPGKRTAQWRNAPLELTSTEFN
                     LLLQLARNAGRVVSKRELSEQALGRSLTRFDRSIDVHLSSIRHKLGRRPDGQSWIETI
                     RGIGYQFIRE"
     gene            2729..3727
                     /locus_tag="PKN55_11770"
     CDS             2729..3727
                     /locus_tag="PKN55_11770"
                     /inference="COORDINATES: protein motif:HMM:NF024890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-hand domain-containing protein"
                     /protein_id="HNX71967.1"
                     /translation="MSMSISSSTSAASAMMFSRTGGARQRPDASQMADDLFSKLDTQN
                     KGYLESSDLASALGSNSSSTSSASAEEVFKSLDSDSDGKLTKTELSDGLKKVQDQLES
                     SFQAMRTKGKGDMPPPPPPQGGNGDADSDTDSSSTSSSQSASYDPADTNKDGTVSATE
                     ALAYALSNSSSTSSSSDASTSSSSTDTTASNTDIANALASMMQNVLQPGQAEGHHGHH
                     GGGKKDEGLTADQMTEIASKTDDSDLASMMKTLASNFEAADVNGDGKVTHDEAMAYQE
                     QNQATGATTTASTSSSSSASALDENVVKQIMQLVQTYSGQSSSKAQSSLGTVSIAA"
     gene            3857..4093
                     /locus_tag="PKN55_11775"
     CDS             3857..4093
                     /locus_tag="PKN55_11775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX71968.1"
                     /translation="MPITSATHTSSPALHAQLSRCQQQLADWMACPSGKTPEGQRTIA
                     SLNTQASELKHALTSTQTSTASPGAVGMQVDTWA"
BASE COUNT          899 a         1353 c         1217 g          693 t
ORIGIN      
        1 agattacccg ctgccctgaa tgcggtctgt gagtgaggcg tgtcggaatg taaccgcgtg
       61 tacatggccg ccaagtgcgc gccacatcat ccgtcacccg ccatcaatgc ggctcggcaa
      121 ctggggtcaa cggcaaggtg atctccaccc gcaggccgcc ttctggccgg ttgcgggcca
      181 caatctgccc gccgtggctt tcgactgcgc ggcgggcaat cgccaaaccc aggccatagc
      241 cggtgccggt cgggttgctc gggctgcggt agaagggctg gaagatggca tccagctcgc
      301 tatccgccac gccggggccg tggtcgctga cgctgatgcg cacttggccg ccctggcatt
      361 ggctttctac ttccacacac tggctttccg gggtgtagcg aatggcgttg cgcacaatgt
      421 tttccaccgc gcgcatcagc aaatccgcac ggccgtgcat atgggcttcg ccgccaccac
      481 gataacgcac gcatttatgc agtgattccg cctcgaactg ggcgtcgcca atcacctcat
      541 ccagcaaggc cagcaagtcg aaggattgtg acgagacgcc gggcaggccg gaatccagcc
      601 tggccagggt cagcacttcg tccaccagct catccagccg ggtgacctcc cgctccaggc
      661 ggtccagtgt ggcgtccagt tgttgcggat tttgccgcac caggccgatg gcggcttgca
      721 gacgcgccag cggtgagcgc agttcgtggg aaatatcatg caataaccgg cgttgcgcgc
      781 caatcagtgc ttccagctgg ccggtcatgc tgtcaaaatc ctgggccagg tcgctgaatt
      841 catcgcgtcg ccgccccatg cgtgcgcgca ctcgggtgtt gagcttgccg gcagacaccg
      901 cacgaaacgc ctggcgcaga tgaccaatcg gtttggccac ataccaggcc aggccggcgc
      961 tgaacgccag gctggccagg atgccggcca gcacatgcac ccagggcagc ggcaggccgg
     1021 ggccgccgcg aaatttgtcg ccgcgctcaa agcctggcgg gccaccgtgc ccatcgcgtg
     1081 gccggccatc gtcgccccgg cggaaattac cgtcgggcaa gggcggcata aagggcagca
     1141 ggccgatgcg cgggcgaaat tcgtggccag gcacaaacag cacataggtg tgctcggacg
     1201 acagggtgac gctgcgcacg cgggcatcgt cgcccttgcc cgccgccagt tgtcgggcgg
     1261 tggcaatggc ctcgggctcg actttgcggc ccaacagctc gcggttttgt tcgtccacgg
     1321 caaaaatcgg cgcttcgcgg ccagaccagt caatcaggat acggcgcagc gtggtttcgt
     1381 cgccatgcac cagcacggat tcagccatgt gcagcagcaa ggcggtgcgc ggccctcccg
     1441 acagtggctg atgctcgtgc ttgggaatat gccaccacac caccagcccg gcgcccacgc
     1501 cagccagcag caaggtcagc cagaaggcca gcaggatttt ccagaacagg ctgcggcgca
     1561 gcaggctggc gcgtggccac agggcggcag gcatcttcac tcccggataa attggtagcc
     1621 aatgccacgg atggtttcaa tccacgactg gccatccggg cggcggccca gtttgtggcg
     1681 gatgctgctc aggtgcacgt caatgctgcg gtcaaaacgc gtcagcgaac gacccagcgc
     1741 ctgctcggac agctcgcgct tgctgaccac ccggccagca ttgcgcgcca gttgcagcag
     1801 cagattgaat tcggtgctgg tcagctccag cggtgcattg cgccattgcg cggtgcgttt
     1861 gcctggccac agtgtcagct cgccaatctg cagcagattg tctgccgact ccgtgggcgt
     1921 gctgttcacc cggcgcaaca cggcgcggat gcgcgccacc agctcgcgcg gggtgcaagg
     1981 cttgggcaca taatcatccg cgcccatttc caggccaatg atgcggtcta catcgtcgcc
     2041 cttggcggtg agcatgatga tcggcgtgct gatttgcgaa cgcacgcggc ccagcacttc
     2101 cacgccgctg gcgcgcggca tcatcacgtc caggacgatc aatgcatatt ggccagacaa
     2161 tgcctcctgg gcgccagctt caccatcgtg agccaaagtc acggcaaagc cctcctgcgc
     2221 gaggtaatcg cgcagcatct cggccagttc gacatcgtca tcgaccagca atagccggtt
     2281 catggcggga tttccttgta aaagcgtcat gtcttgcatg ataacggttc ctgattggcg
     2341 ctctgagcgg ctttgccagg ctttacccag ctttacatag cctgactgac ggcaaaaatc
     2401 gccatcggca cggatggcgg ctatctggcg aatgttaaat ccattgtgca tcagccattt
     2461 gcccagcagc gtgttttggc aggatttgcc aggcctggcg gcaaaaactg ccagcaggcc
     2521 catccacgcc aggggtgaat gtttccgcca tcgaccccgc gcggcaaaac tcgatgacat
     2581 ttaacgcaaa tttacccgac tttaccaaca acaaccttta atttcatgaa cttaggtgag
     2641 attcttgcaa tcacgcagcc ggcagtcatc gctttggcgc atgcactgcc cacctaccca
     2701 ccgtgacatg cttataagga gcctcaccat gtcgatgagc atctcaagca gcaccagcgc
     2761 cgcctcggcc atgatgtttt cccgcacggg cggcgcgcgc cagcgccccg acgccagcca
     2821 gatggcggac gacctgtttt ccaaactgga cacccaaaac aaaggttatc tggaatccag
     2881 cgacctggcc tcggccttgg gcagcaacag cagctcgacc agcagcgcca gcgctgaaga
     2941 ggtgttcaaa tcgctggaca gcgacagcga cggcaagctg accaaaaccg agctgagcga
     3001 cggcctgaaa aaagtgcaag atcagctgga gagcagcttc caggccatgc gcaccaaggg
     3061 caagggcgat atgccgcccc cgccgccgcc gcaaggtgga aatggtgatg cagacagcga
     3121 caccgacagc agcagcacca gcagcagcca atccgccagc tatgacccgg ccgacaccaa
     3181 taaagacggc acagtcagcg ccaccgaagc cttggcttac gcgctgtcca attccagcag
     3241 cacctccagc agcagcgatg cctcgaccag cagcagctcg accgacacca ccgccagcaa
     3301 caccgatatc gccaatgcac tggccagcat gatgcagaac gtgctgcaac cgggccaggc
     3361 tgaaggccat cacggccacc atggcggcgg caagaaggac gaaggcctga cggcagatca
     3421 aatgaccgaa atcgccagca agacggacga tagcgacctg gccagcatga tgaaaaccct
     3481 ggcgagcaac tttgaagccg ctgacgtaaa tggtgacggc aaagtgaccc acgacgaagc
     3541 catggcttac caagagcaaa accaggccac cggcgccacc accacggcca gcaccagctc
     3601 aagcagcagc gccagcgcgc tggatgagaa tgtggtgaag cagatcatgc agctggtgca
     3661 aacctacagc ggccaatcca gcagcaaggc acaaagttcg ctgggcacgg tgtccatcgc
     3721 cgcctgatga ttcacgtgaa acatctggcg tgaacgcaaa aaaccctcgg gcaaccgagg
     3781 gttttttgtg ggctgatggt aaggctggcg cagtggcaac gacagatagc atgcacaccc
     3841 ttaccaggag aacagcatgc caatcacctc tgccacccac accagcagcc ccgccctgca
     3901 tgcacaactg agccgttgcc aacagcaact ggccgactgg atggcttgcc catcaggcaa
     3961 aacaccagaa ggtcagcgca ccattgccag cttgaatact caagccagtg agctcaagca
     4021 cgcactgaca agcacgcaaa cctcaacggc cagccccggc gccgttggca tgcaggtgga
     4081 cacctgggca tagccaggtg tatgcggcgc ttacagcttg tcgtacagct tgccgccgcc
     4141 cttcacaaac tccaccgact tc
//
LOCUS       DAOPYX010000265         4156 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_27950_length_4156_cov_12.953670, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000265 DAOPYX010000000
VERSION     DAOPYX010000265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 4156)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4156)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4156
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27950_length_4156_cov_12.953670"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..747
                     /locus_tag="PKN55_11780"
     CDS             <1..747
                     /locus_tag="PKN55_11780"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008954328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006528 - asparagine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II asparaginase"
                     /protein_id="HNX71969.1"
                     /translation="DVDGIVITHGTDTLEETAYFLNLTVKSKKPVVLVGAMRPSTAIS
                     ADGPINLYNAVALASSKDAAGRGVLVSLNDQINAARDVTKSNTMTTDTFRSADLGALG
                     YMVSGKPMFYRGTARKHTTDSEFDVSKLDKLPAVNVVYSYANATRTAIDAFAAAGDVG
                     IVHAGTGNGSLSTPVKAALTEARKKGMIVVRSSRTGTGITARNGEANDDELDFVAADN
                     LSPQKARILLMLGLTKTQNTKDLQRMFMMY"
     gene            957..2984
                     /locus_tag="PKN55_11785"
     CDS             957..2984
                     /locus_tag="PKN55_11785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011137852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HNX71970.1"
                     /translation="MHTDPYLWLEDDSAATRAWCAEQQARTAAQLDADPRFAALADEV
                     LEHLRDDERVPFVEAHAGWWHHFLQSPAHPRGVYRRIRPDDYRANRDAWEVLFDLDAL
                     AAAEGVDWHLAGIDHCTERPARCLLSLSADGGDAACLREYDLDARAFVAEGFQLPPGK
                     HSVTWRDPDSLYAAVVLSEADATASGYPREVKLWQRGQPFAQAHSLLKAEHRALAVSA
                     WRMLNPDGGAPVDIIEEALSFYRTRYWRVLPDLSRQALPLPPKVDIDGVVAGQLIVRL
                     NQAWGDLDAGVLLALDWQALCAGDIDPLPLFTPGPRQSVDGALAGRSCLFVHVLDEGV
                     SQLWVYTPTADGWQAQRAPLPGQGQLEMVDQPWQSNVLLFGYDDFLTPSTLYWLDADD
                     ASLTPQALRGQPPRFAAEQMHAQLRHAIAPDGERIPYFVVARRDAPHDGQRPTLLYGY
                     GGFAVPMTPFYLGALGRQWLEQGGVFVLSCIRGGGEFGPDWHEAALAERRQVAFDDFL
                     AIARQLIADGVTCPARLGIQGGSNGGLLVAAAMTQAPELFGAVVCEVPLTDMLRYTEL
                     SAGPSWIDEYGDPADPEHHAALAAYSPYQHLQAGVRYPATLVTTSSSDDRVHPGHARK
                     FTARLGELGQPVLLYQSDAGGHSGQGGDMRQLAAEVARVTVFLYQQLMDAA"
     gene            2984..4114
                     /locus_tag="PKN55_11790"
     CDS             2984..4114
                     /locus_tag="PKN55_11790"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009118859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HNX71971.1"
                     /translation="MKIVICPDSFKESLPADAAAQAIAEGVRELWPDAECVCLPLADG
                     GEGTLDALVSATGGQLLRHTVRGPLGAPVDARFAVLGDGETALIEMAEAAGLMRIPRG
                     ERDPLRASTYGVGELIAAALAQGARRILLGLGGSATQDGGAGMLQALGARLLDADGQP
                     LPPGGAALAQLATLDLAPMQAALAGVQVAVACDVDNPLCGPRGASVVFAPQKGASPAE
                     VAQLDAALAHWGRYLAQACGREVAALPGAGAAGGMGAAALAAFDARLRPGIDWVMDAV
                     RLDEALRGADWVISGEGRADGQSAGGKVLSGVARRAQAAGVPVLALVGCLGEGYEALY
                     PLGVRSILPIVPGPISLDEALAQAAANLRRTARAAAGLWRQG"
BASE COUNT          603 a         1437 c         1462 g          654 t
ORIGIN      
        1 gacgtggacg gcatcgtcat cacccacggc accgacaccc tggaagaaac cgcctacttc
       61 ctgaacctga cggtcaagag caaaaagccg gtggtgctgg ttggcgccat gcgcccgtcc
      121 accgccatca gcgccgacgg cccgatcaat ctgtataacg ccgtggccct ggccagcagc
      181 aaggacgccg ccggccgtgg cgtgctggtg tcgctgaacg accagatcaa cgccgcccgc
      241 gatgtgacca agagcaacac catgaccacc gacaccttcc gcagcgccga cctgggcgcg
      301 ctgggctata tggtgtcggg caagccgatg ttctatcgcg gcaccgcgcg caagcacacc
      361 accgacagcg aatttgacgt cagcaagctg gacaagctgc cggccgtcaa cgtggtgtac
      421 agctacgcca acgccacccg caccgccatt gacgcctttg ccgccgccgg cgacgtgggc
      481 attgtccacg ccggcaccgg caacggcagc ctgtccaccc cggtgaaggc cgcgctgacc
      541 gaagcccgca agaagggcat gattgtggtg cgtagctcgc gcaccggcac cggcatcacc
      601 gcccgcaatg gcgaagccaa cgatgacgag ctggattttg tcgctgctga caacctgtcc
      661 ccgcaaaaag cccgcatcct gctgatgctg ggcctgacca agactcagaa caccaaagac
      721 ctgcaacgca tgttcatgat gtactgatcc cgagccgccg cgcccatggg cgtggcggtg
      781 aggtgtgagt gtgattcatc cctctcgggt ctggacacgg atggggggcg gcctgcgggc
      841 cgaacagaag tgcacccagc cccgaatgct tgccccgccg gctctccccg gcgggttttt
      901 tgcgcctgga tttctcgcgg tacaatccgg ccattctgac ttttcctggc tctctcatgc
      961 acaccgaccc ctacctctgg ctggaagacg attccgccgc cacccgcgcc tggtgcgccg
     1021 agcaacaggc gcgcaccgcc gcccagctgg acgccgaccc gcgctttgcc gcgctggccg
     1081 acgaagtgct ggagcatctg cgcgacgatg agcgcgtgcc ctttgtcgag gcgcatgccg
     1141 gctggtggca ccactttttg caaagcccgg cgcatccgcg cggggtgtac cgccgcattc
     1201 gccccgacga ctaccgcgcc aaccgcgacg cctgggaagt gttgttcgac ctggacgcgc
     1261 tggccgccgc cgagggcgtg gactggcatc tggccgggat tgaccattgc accgagcggc
     1321 cggcgcgctg tttattgtcg ctgtcggccg atggcggcga cgccgcctgc ctgcgcgaat
     1381 acgacctgga cgcgcgcgcc tttgtcgccg agggttttca gctgccgccc ggcaagcaca
     1441 gcgtcacctg gcgcgacccg gacagcctgt acgccgccgt ggtgctgagc gaagccgacg
     1501 ccaccgcctc gggctatccg cgcgaggtca agctgtggca acgcggccag ccgtttgccc
     1561 aggcgcacag cctgctgaaa gccgagcatc gcgccctggc ggtcagcgcc tggcgcatgc
     1621 tcaacccgga cggcggcgcg ccggtggaca tcatcgaaga agcgctgagc ttttaccgca
     1681 cccgctactg gcgcgtgctg cccgacctca gccggcaggc gctgccgctg ccgcccaagg
     1741 tggacatcga cggcgtggtg gccggccagc tgattgtgcg gctgaaccaa gcctggggcg
     1801 atctggacgc cggcgtgctg ctggcgctgg actggcaggc gctgtgcgcc ggcgacatcg
     1861 acccgctgcc cttattcacc cctggcccgc gccagtcggt ggatggcgcg ctggccgggc
     1921 gcagctgcct gtttgtgcat gtgctggacg aaggcgtcag ccagctgtgg gtgtacaccc
     1981 ccaccgccga cggctggcag gcgcagcgcg cgccgttgcc cggccagggc cagctggaga
     2041 tggtggacca gccgtggcag agcaatgtgc tgctgtttgg ctacgatgat tttctcaccc
     2101 cctccaccct gtactggctg gacgccgacg acgccagcct gacgccgcag gcgctgcgcg
     2161 gccagccgcc gcgctttgcc gccgagcaga tgcacgcgca gctgcgccac gccatcgccc
     2221 cggacggcga gcgcatcccg tattttgtcg tggcccgccg cgacgcgccg cacgacggcc
     2281 agcgcccgac cttgctgtac ggctacggcg gttttgccgt gccgatgacg ccgttttacc
     2341 tgggcgcgct gggccggcag tggctggagc aaggcggcgt gtttgtgctc agctgcattc
     2401 gcggcggcgg cgaatttggc ccggattggc acgaggcggc gctggccgag cgccggcaag
     2461 tggcgtttga tgattttctg gcgattgccc gccagctgat cgccgacggc gtgacctgcc
     2521 cggcgcggct gggcattcag ggcggcagca atggcggcct gttggtggcg gcagcgatga
     2581 ctcaggcgcc cgagctgttt ggcgcggtgg tgtgcgaagt gccgctgacc gatatgctgc
     2641 gctacaccga gctgtccgcc ggcccgagct ggatcgatga atacggcgac ccggccgacc
     2701 ctgagcacca cgccgcgctg gcggcctatt cgccgtatca gcacctgcaa gccggcgtgc
     2761 gctacccggc caccctggtg accacctcca gcagcgacga ccgcgtgcac cccggccacg
     2821 cgcgcaagtt caccgcccgc ctgggcgagc tgggccagcc ggtgttgttg tatcagtccg
     2881 acgccggcgg ccacagcggc cagggtggcg atatgcgcca gctggcggcg gaagtggccc
     2941 gagtgacggt gtttctgtat caacagctga tggatgccgc ctgatgaaaa tcgtgatctg
     3001 cccggacagc ttcaaagaaa gcctgccggc cgacgctgcc gcccaggcga ttgccgaggg
     3061 cgtgcgcgag ctgtggccgg atgcggagtg tgtgtgcctg ccgctggccg acggcggcga
     3121 aggcacgctg gatgcgctgg tcagcgccac tggcggccag ttgctgcgcc acaccgtgcg
     3181 cggcccgctg ggcgcgccgg tcgatgcgcg ttttgccgtg ctgggcgacg gtgaaacggc
     3241 cctgattgaa atggccgagg ccgccggcct gatgcggatt ccgcgcggcg agcgcgaccc
     3301 cttgcgcgcc agcacctacg gcgtgggcga gttgatcgcc gccgcgctgg cgcagggcgc
     3361 gcggcgcatc ttgctgggcc tgggcggcag cgccacccag gatggcggcg ccggcatgct
     3421 gcaggcgctg ggcgcgcgcc tgctggatgc cgacggccag ccgctgccgc ccggcggcgc
     3481 ggcgctggcg caactggcca cgctggactt ggcgcccatg caggcggcgc tggctggcgt
     3541 gcaggtggct gtcgcctgcg atgtggataa cccgctgtgc ggcccgcgcg gcgccagcgt
     3601 ggtgttcgcc ccgcaaaaag gcgccagccc ggccgaggtg gcgcaactgg acgccgcctt
     3661 ggcgcactgg ggccgttatc tggcgcaggc ctgcggccgc gaagtggccg cactgcccgg
     3721 cgcgggcgcg gccggcggta tgggcgctgc cgcgctggcg gcgtttgacg cccgtctgcg
     3781 tcccggcatc gactgggtga tggacgccgt gcggctggat gaagcgctgc gcggcgccga
     3841 ctgggtaatc agcggcgaag gccgcgctga tggccaatcg gccggcggca aagtgctcag
     3901 cggtgtcgcc cgccgcgccc aggccgcggg cgtgccggtg ctggctttgg tgggctgtct
     3961 gggcgaaggc tatgaggcgc tctacccgct gggcgtgcgc tccattttgc ccatcgtgcc
     4021 cggcccgatc agcctggacg aggcgctggc gcaggcggcg gccaatctgc gccgcaccgc
     4081 gcgcgcggcg gcggggttgt ggcggcaggg gtagtgtatt gtttggcggt tgagaccgct
     4141 cacaaaaccc cctggc
//
LOCUS       DAOPYX010000266         4155 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_27965_length_4155_cov_9.432439, whole genome shotgun sequence.
ACCESSION   DAOPYX010000266 DAOPYX010000000
VERSION     DAOPYX010000266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 4155)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4155)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4155
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27965_length_4155_cov_9.432439"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1586)
                     /locus_tag="PKN55_11795"
     CDS             complement(<1..1586)
                     /locus_tag="PKN55_11795"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNX71972.1"
                     /translation="MQPMPSVTPMSTERPFPRGQVVVCQYDFNFHLTYVNPAFARMLG
                     YSREELVGQPLKRIAHPGIPQALLDDIRATTGRGQPWRGMAKTLRRDGGYVWSESLII
                     PVLKRGEVTGYMSIRSEASPQRIAAEEQRYRDIHAGARRYHEGRGPDWTRVTAFHLLA
                     ALGAAATLSALCAGLLWWAPAWLAGREGGLAALAGGCWLLTLLSARWLGRRTLRGLGD
                     IIGHFHRMAEGDLSHEIPVGGQDEVGRVLEALAVMQGHLQVLLDEVGAAAHTTEQENL
                     ALLADIDALGQSAQQRLDEMAQLRAISADNLAAVGRADADAQACAEAARDSLALAAEG
                     RRRLTMAADAAADARATVDAASDTLNQLEDALRQVEKMSALIGDIAAQTHLLALNAAI
                     EAARAGESGRGFAVVADEVRKLAEHTSGSIASISQRAGDIRAVSQHARHSMQAAARHT
                     HSASAAVDASRDTVAAMAGHSEAMSARVGQIAQACAALSEAVTVTRQQVETAHQQTGC
                     DQHRLTAVNAGVTAVRGEIAA"
     gene            complement(1692..2843)
                     /locus_tag="PKN55_11800"
     CDS             complement(1692..2843)
                     /locus_tag="PKN55_11800"
                     /inference="COORDINATES: protein motif:HMM:NF024996.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HNX71973.1"
                     /translation="MTLNRTLLALTLSGLSAAALADVTLYGAIDETLESVSARGAADS
                     ARNIGQTTRINSNSSLIGFKGAEDLGNGLKAIWQVESGLAIDTGGGNFGTRDTFIGLT
                     SPSMGTLQLGLLTSSARAMGGIFDINKAGTGIGMSAALLGKLGNLAVTDAALKTQAAG
                     QIGLFDNRLRNSVQYTSPVFSNFSASALYSTGENGVGPRAYNANIGVKYDDGAWFAAL
                     AYTRSTTGADNSSATSVSAFEKVSDLRAALTYRFSANTRIGLMYDRTDGNLTRAGSLA
                     YGGSDLKQSVWYVNGALGIGANGRIIAQYGQAGKLSGGSAALADSGKARHYTLGYEHD
                     LSKRTLFKAIYSEIRNNRAANYDFGIGSIGGVAAGADPRGFAVGLRHTF"
     gene            complement(2895..3680)
                     /locus_tag="PKN55_11805"
     CDS             complement(2895..3680)
                     /locus_tag="PKN55_11805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014087925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HNX71974.1"
                     /translation="MSYSTLEIQRDGAVATLWMNRPEVFNAFDEQLIAELTQACQALD
                     HDDSVRVVVLAGRGRHFSAGADLNWMRRAASHGEAENLADARQFARMLRTLATLSKPT
                     VARVHGAALGGGAGLTAACQMAIASDDAVFSTSEVRFGIIPAVISPYVLQAIGPRQAQ
                     RYFQSGERFDAARALAMGLVSEVAPAAQLDARVAALAESLCAGGPCAQRAARELIAAV
                     HGRPLGEATDEDTAQRIARQRATAEAREGIAAFLEKRPADWLR"
     gene            complement(3689..>4155)
                     /locus_tag="PKN55_11810"
     CDS             complement(3689..>4155)
                     /locus_tag="PKN55_11810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019923880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HNX71975.1"
                     /translation="FALGGGCELAMMCDMIFAADTAKFGQPEIRLGTMPGAGGTQRLP
                     RAVGKAKAMDLCLSARMMDAAEAERAGLVARLYPAAQLLEETLKAAQTIAGFSLPVTM
                     MIKESVNRAFESSLNEGLLFERRVFHATFTLDDQREGMQAFADKRKPVFRHQ"
BASE COUNT          670 a         1402 c         1391 g          692 t
ORIGIN      
        1 gcggcgattt cgccgcgcac tgcggtcacg ccggcattca ccgccgtcag gcgatgctgg
       61 tcgcagccgg tttgctggtg ggcggtttcc acctgttggc gggtgacggt gaccgcctcg
      121 ctcagcgccg cgcaggcctg ggcaatctgg cccactcgcg cgctcatcgc ctcgctatgg
      181 ccggccatgg ccgccaccgt gtcgcggctg gcgtccaccg ctgcgctggc gctgtgcgtg
      241 tggcgggcgg cggcctgcat gctgtggcgc gcgtgctggc tgaccgcgcg gatatcgccg
      301 gcgcgctggc tgatcgaggc aatgctgccg ctggtgtgct cggccagctt gcgcacttcg
      361 tcagccacca cggcaaatcc ccggccagat tcacccgcgc gggcggcttc gatggcggcg
      421 ttcagcgcca gcaaatgggt ttgcgcggcg atgtcgccaa tcagcgccga cattttttcc
      481 acctgacgca aggcgtcttc cagttggttg agcgtgtcgc tggcggcgtc caccgtggcg
      541 cgggcgtcgg cggcggcgtc tgccgccatg gtcagccgac gacgcccttc cgccgccagc
      601 gccagcgaat cgcgcgcggc ctcggcgcag gcttgcgcgt cggcgtcggc gcggcccacc
      661 gccgccagat tgtcggcgct gatggcgcgc agctgcgcca tttcgtccag ccgctgctgc
      721 gcggactggc ccagggcgtc aatgtcggcc agcaaggcca ggttttcttg ctcggtggtg
      781 tgcgccgctg cgccgacttc gtcgagcagc acctgcaaat gcccttgcat caccgccaat
      841 gcctccagca cgcggcccac ttcgtcctga ccgcccaccg ggatttcatg gctgaggtcg
      901 ccctcggcca tgcgatgaaa atggccaatg atgtcgccca gcccgcgcag cgtgcgccgc
      961 cccagccagc gcgcgctcag cagggtcaag agccagcagc cgccggccag cgccgccagc
     1021 ccgccttcgc gcccggccag ccaggccggc gcccaccaca gcaggccggc gcacagggcc
     1081 gacaaggtgg ccgccgcgcc cagcgccgcc agcaggtgaa acgccgtcac ccgcgtccag
     1141 tctggcccgc gtccttcgtg ataacggcgc gcgccagcgt ggatgtcgcg gtagcgttgt
     1201 tcttcggcgg cgatgcgctg cgggctggcc tcgctgcgga tcgacatata gccggtgact
     1261 tcgccgcgct tgagcaccgg gatgatcaag gactccgacc acacatagcc gccatcgcgg
     1321 cgcaaggttt tggccatgcc gcgccagggc tggccgcgcc cggtggtggc gcggatgtcg
     1381 tccagcagcg cctgcggaat gccggggtgg gcaatgcgtt tcagcggctg gcccaccagt
     1441 tcttcgcggc tgtagcccag catgcgggca aacgccgggt tgacgtaggt cagatgaaaa
     1501 ttgaagtcat actggcacac caccacctgg ccgcgcggaa acggccgctc ggtggacatc
     1561 ggcgtgacgg acggcatcgg ctgcatggcc aactccttgc agaacggata aaaaaagacc
     1621 tccgactgcc ggcggaggcc caagctgggg aaacgcaacg ccacccaatc aggcagacgc
     1681 tgaaacaccc atcagaaggt atggcgcaag ccgacggcaa agccgcgcgg atccgcgccg
     1741 gccgccaccc cgccaatgct gccgatgcca aagtcgtaat tggccgcgcg attattgcga
     1801 atctcgctgt aaatcgcctt gaacagcgtg cgcttggaca aatcatgctc atagcccagc
     1861 gtgtagtgac gcgccttgcc gctatcggcc aatgccgcgc tgccgccgct gagtttgcca
     1921 gcctgaccgt actgggcaat gatgcggcca ttggcgccaa tgcccagcgc gccgttgaca
     1981 taccacaccg actgcttcag atcgctgccg ccataggcca gcgagcctgc gcgggtcaga
     2041 ttgccgtcgg tgcggtcata catcaggccg atgcgggtgt tggccgaaaa gcgataagtc
     2101 aacgccgcgc gcagatcgct gactttttcg aaagcactga cgctggtcgc cgagctgttg
     2161 tccgcgccgg tggtcgagcg ggtgtaagcc agtgcggcaa accaggcgcc gtcgtcgtac
     2221 ttcacgccga tattggcgtt gtaagcacgc gggccgacgc cattctcgcc cgtgctgtac
     2281 agcgcgctgg cggaaaagtt gctgaacacc gggctggtgt actgcaccga attgcgcagg
     2341 cggttatcaa acaaaccaat ctgaccggca gcctgggttt tcagcgcggc atcagtcacc
     2401 gccagattgc ccaacttgcc caacaacgcc gccgacatgc caatgccggt gccggccttg
     2461 ttgatatcaa aaatcccgcc catcgcgcgg gcggacgaag tcagcaaacc caattgcagc
     2521 gtgcccatgc tcgggctggt caggccgata aaggtgtcgc gggtgccaaa attgccgccg
     2581 ccagtgtcga tggccaggcc gctctccacc tgccagatgg ctttcaggcc attgcccaga
     2641 tcttcagcgc ccttgaaacc gatcaaggag ctattggaat taatccgggt ggtctggccg
     2701 atattgcgcg cgctgtccgc cgcgccacgg gcggacacgc tctccagggt ttcgtcgatg
     2761 gcgccgtaca gggtgacgtc ggccagcgcg gcggcggaca ggccgctgag ggtgagggcc
     2821 agcagggtgc gattcagggt catgggtgtc ttcctccaat ggttgttgtg gtgaacagca
     2881 gaatccggca aggctcagcg cagccagtcg gccgggcgtt tttccagaaa ggcggcgatg
     2941 ccttcgcgcg cttcggcggt ggcgcgctga cgggcaatgc gctgggcggt gtcttcgtcg
     3001 gtggcttcgc ccagcgggcg gccgtgtacg gcggcaatca gctcgcgggc ggcgcgttgt
     3061 gcgcacgggc cgcccgcgca cagcgactcg gccagcgccg ccacccgtgc gtccagctgg
     3121 gcggccggag ccacctcgct gaccaggccc atggccagcg cgcgcgcggc gtcaaaacgc
     3181 tcgccgcttt gaaaatagcg ctgcgcctgg cgcgggccga tggcctgcaa cacatagggg
     3241 ctgatcaccg ccgggatgat gccaaagcgc acttccgagg tagaaaacac cgcgtcatcg
     3301 ctggcgatag ccatctggca ggcggcggtc agcccggcgc cgccgcccag cgccgcgccg
     3361 tgcacgcggg cgacggtggg tttggacaag gtggccaggg tgcgcagcat gcgggcaaac
     3421 tggcgggcgt cggccaggtt ttctgcctcg ccgtggctgg cggcgcggcg catccagttc
     3481 aggtcggcgc ccgccgaaaa atgccggccg cgcccggcca gcaccaccac gcgcacgctg
     3541 tcgtcgtggt ccagcgcctg gcaggcctgg gtgagctcgg cgatcaactg ctcgtcaaag
     3601 gcgttgaaca cttccgggcg gttcatccac agcgtggcca ctgcgccgtc gcgctggatt
     3661 tcaagggtgg aataagacat ggctctctct attgatggcg gaacaccggc ttgcgcttgt
     3721 cggcaaacgc ctgcatgcct tcgcgctggt catccagggt gaaggtggcg tgaaacaccc
     3781 gacgctcgaa cagcaggcct tcgttcaggc tgctttcaaa ggcgcgattg accgattcct
     3841 tgatcatcat cgtcaccggc agcgaaaagc cggcgatggt ctgcgcggcc ttcagggttt
     3901 cttccagcag ttgcgccgcc gggtagaggc gcgccaccag gccggcgcgc tcggcttcgg
     3961 cggcgtccat catgcgcgcg ctcaggcaca ggtccatggc cttggccttg cccaccgcgc
     4021 gcggcaggcg ctgggtgccg ccggcaccgg gcatggtgcc caggcggatt tccggctggc
     4081 cgaatttggc ggtgtcggca gcaaaaatca tgtcgcacat catcgccagc tcgcagccgc
     4141 cgcccagggc aaagc
//
LOCUS       DAOPYX010000267         4155 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_27966_length_4155_cov_9.223902, whole genome shotgun sequence.
ACCESSION   DAOPYX010000267 DAOPYX010000000
VERSION     DAOPYX010000267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 4155)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4155)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4155
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27966_length_4155_cov_9.223902"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            184..1218
                     /locus_tag="PKN55_11815"
     CDS             184..1218
                     /locus_tag="PKN55_11815"
                     /inference="COORDINATES: protein motif:HMM:NF018009.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDDEXK nuclease domain-containing protein"
                     /protein_id="HNX71976.1"
                     /translation="MSEKPVRLTEPPAGYAAWLTELKARIHTAQQRATLAVNRELVLL
                     YWQIGRDILARQAEQGWGAKVIERLAEDLRLAFPEMKGFSPRNLKYMRAFAQAWPDEA
                     IVQEALAQLPWYHHLALLDKLPDSETRRWYAAKAIEHNWSRNILVMQIETRLLERSGQ
                     AVSNFPMTLPKPQSDLARESLKDPYRFDFLGLTDEAQEREIEAALVKHVTEFLLELGA
                     GFAFVGRQVLLDVGGDEFFIDLLFYHLKLRCYVVIELKGGKFKPEHLGQLGFYMTAVD
                     RQIKHEQDNPTIGLLLCKSKNKVVAEYALGDKSQPMGIAEYKMLESLPAELQTSLPSI
                     EQIERELMGG"
     gene            complement(1284..1739)
                     /locus_tag="PKN55_11820"
     CDS             complement(1284..1739)
                     /locus_tag="PKN55_11820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX71977.1"
                     /translation="MSMESDDVFWERLRGIAEALPEWLKINREALMAQIIRSVLNCFR
                     AQPSPEQIEGIETGLDFLGLLLYEGGDNTLCEVTMEQLDSEIYRAVARLSDDEQIVLL
                     LPLCDGIDDLATDRDIEEWARFLRMDSAWQAEIRCAVLNRVEPRNRYAD"
     gene            1974..>4155
                     /locus_tag="PKN55_11825"
     CDS             1974..>4155
                     /locus_tag="PKN55_11825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014086644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HNX71978.1"
                     /translation="MSHFLDRLNFLGKVKATFADGHGAVVKEDRKWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKVYVKNGLITWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     VYSAQRVKYPMVRGVLMQHWRELRKTLGPIQAWQRISQTPEIASQYKTQRGQGGFVRA
                     SWDEVNEIIAASNAYTIKNYGPDRVIGFSPIPAMSMVSYAAGSRYLSLIGGVPLSFYD
                     WYCDLPPASPQIWGEQTDVAESADWYNSNYLMVWGSNIPMTRTPDAHFYTEVRYKGTK
                     TVAVSSDFGEMAKFGDIWLAPKQGTDAALAMAMGHVIFKEFHLERPSEYFTDYIRRLT
                     DMPMLVTLKQDGSRYVPEYFLRASQLTGNLGEDNNPEWKTLAFDELSGDLVAPNGSVG
                     FRWGQSGKWNLEARESGGREVRARLSLLEQRDAVAAVAFPYFGAEHDEVMVRNVPARK
                     VQLANGEEVLVATVFDLMAANYGIDRGLGGGNVASSYLDDVPYTPAWQQKHTGVKPEM
                     VIQVAREFAQNADQTHGRSMVIVGAALNHWYHMDMTYRGIINMLMLCGCIGQSGGGWC
                     HYVGQEKLRPQTGWAPLAFAADWNRPARQMNGTSFFYAHTSQWRHEKLGVNEILCPTA
                     DGKMANMALIDYNAKAERMGWLPSAPQLGANPLDLTRQAEAAGKDPIAYAVEQLKTGQ
                     LDMACNDPDNPQNFPRNLFVWRSNILGSSGKGHEYFLKYLLGTQN"
BASE COUNT          891 a         1209 c         1233 g          822 t
ORIGIN      
        1 tgcggagcat atggacgcat tcgccgcagc gcgggcatac tcgatacacc cagtgaaaca
       61 gaaaaaccct tgagcaatca gcgcataacg cccttctggc ccagctgcac cgcccagcat
      121 gcagccccgc caagcacgct gtgctgagta tgattgactg cgccaagcaa gagacaacac
      181 accatgagtg aaaaacccgt tcgcctgacc gagccgccag caggctacgc cgcttggctg
      241 accgagctga aagcccgcat ccataccgct caacaacgcg ccaccctggc cgtgaatcgt
      301 gaactggtgc tgctgtattg gcaaatcggg cgcgacatcc tggcccgaca agcagaacaa
      361 ggctggggag ccaaggtgat cgaacggctg gccgaggacc tgcgtttggc ttttccggag
      421 atgaaggggt tttcgccgcg caatctcaag tatatgcgtg cttttgccca ggcttggccg
      481 gatgaagcaa ttgtgcaaga ggcgcttgca caattgccct ggtatcacca tttggcgctg
      541 ctggataagt tacccgactc agaaactcgc cgctggtacg ccgccaaagc cattgagcac
      601 aactggtcgc gcaatatcct ggtgatgcag atcgaaaccc gcctgctgga gcgcagcggc
      661 caggcggtgt ctaactttcc catgaccttg cccaagccac agtctgatct ggccagagaa
      721 tcactgaaag acccgtaccg tttcgatttt cttggtctga ccgatgaagc gcaagagcgc
      781 gaaatcgaag ccgccctggt caaacacgtc accgaattcc tgctggagct gggcgcaggc
      841 tttgcctttg ttggccgcca agtgctgctg gacgtgggcg gcgacgagtt ttttatcgac
      901 ctgctgttct accacctcaa gctgcgctgc tatgtggtga tcgaactgaa aggcggcaaa
      961 ttcaagccag aacacctggg gcaactgggc ttttatatga ccgccgtaga ccgccagatc
     1021 aaacacgaac aagacaaccc caccatcggc ctgctgctgt gcaagagcaa aaacaaagtg
     1081 gtggcggaat atgcgctggg agacaaaagc cagccgatgg gcattgccga atacaagatg
     1141 ctggaatccc tgcccgctga gctacaaacc agcttgccga gcattgagca gattgagcgt
     1201 gagttgatgg gggggtgagt ccatggtggc accttggcag agtgttgcgc ttggttcttg
     1261 agaccgtcct atgcttgtac tgatcaatca gcatacctat tgcgaggttc aaccctattc
     1321 aacacagcac aacggatttc tgcttgccat gcactatcca tccgcaggaa ccgagcccat
     1381 tcttcgatat cgcgatcagt tgctaaatca tctatgccgt cacatagtgg cagcagtaga
     1441 acaatctgtt cgtcatcgct aaggcgtgct acagcacgat aaatttcgct atcaagttgt
     1501 tccattgtca cttcacacaa cgtattgtcg ccaccttcgt acaacagcag gcctagaaaa
     1561 tccagtccag tttcgatgcc ttctatctgt tctggcgatg gctgagcacg aaaacagttc
     1621 agtactgatc tgatgatctg cgccatcaag gcctcacggt tgattttcag ccattccggc
     1681 aacgcctcgg caataccacg cagccgttcc caaaaaacat catcgctttc catgctcatg
     1741 tagtttctta ttcaagaggc cacattaaac aagaatttag gagagatggg tagaggcatt
     1801 gttgcacaaa atatagagca cacacctccc cctcctttcg aactacccca ccctcccccc
     1861 tctgcctcgc ccgcgctctc cctctgacca atgggcggtt tgcgtgcagc ttcttactct
     1921 tggcggtatc ggcgccgggc tgtctggccc ggccaacccc cttggaggca cgcatgagcc
     1981 attttctcga cagactgaat tttctcggca aggtgaaagc cactttcgcc gatggccacg
     2041 gtgcggtggt gaaggaagac cgcaagtggg aggacggata ccgccagcgc tggcagcacg
     2101 acaagatcgt gcgctccacg catggggtga actgcaccgg gtcgtgcagt tggaaggtgt
     2161 acgtcaaaaa cgggctgatc acctgggaga cgcagcagac cgactatccg cgcacgcggc
     2221 cggatttgcc caaccacgag ccgcgcggtt gtccgcgtgg ggcgtcgtat agctggtatg
     2281 tgtattcggc gcagcgggtg aagtacccga tggtgcgcgg ggtgttgatg cagcactggc
     2341 gcgagctgcg caaaacgctg gggccgattc aggcctggca gcggatcagc cagacgccgg
     2401 aaatcgcctc gcagtacaaa acccagcgcg gccagggcgg ctttgtgcgc gccagctggg
     2461 acgaggtgaa cgagatcatc gccgcctcca atgcctacac catcaagaat tacggcccgg
     2521 atcgggtgat tggtttttcg ccgattccgg cgatgtcgat ggtgtcttac gccgctggca
     2581 gccgttacct gagcctgatt ggcggcgtgc cgctgtcgtt ttacgactgg tactgtgatt
     2641 tgccgccggc cagcccgcag atttggggcg agcagaccga cgtggccgag tcggccgatt
     2701 ggtataactc gaattacctg atggtgtggg gttcgaatat cccgatgacg cgcacgccgg
     2761 atgcgcattt ttacaccgag gtgcgctaca agggcacgaa aacggtggcg gtgtcgtcgg
     2821 actttggcga gatggccaag tttggcgata tctggctggc gcccaagcag ggcactgatg
     2881 cggcgctggc gatggcgatg ggccatgtga ttttcaagga attccacctt gagcggccca
     2941 gcgagtattt caccgactac atccgccggc tgaccgatat gccgatgctg gtgacgctca
     3001 agcaggatgg cagccgttat gtgccggaat acttcctgcg cgcttcgcag ctgaccggca
     3061 atctgggcga ggacaataac ccggaatgga aaaccctggc gtttgacgag ctcagcggtg
     3121 atctggtggc gcccaatggc tcggtgggct tccgctgggg gcagtcgggc aagtggaacc
     3181 tggaggcgcg ggaaagcggc ggccgtgagg tgcgcgcccg tttgtcgctg cttgagcagc
     3241 gcgacgcggt ggcggcggtg gcttttccgt attttggcgc cgagcacgac gaggtgatgg
     3301 tgcgcaatgt gccggcgcgc aaggtgcagc tggccaatgg cgaagaagtg ctggtggcca
     3361 cggtgttcga cctgatggcg gccaattacg gcattgatcg cggcctgggc ggcggcaatg
     3421 tcgccagctc gtatctggat gatgtgccct acaccccggc ctggcagcaa aagcacaccg
     3481 gggtgaagcc agaaatggtg atccaggtgg cgcgcgaatt cgcccagaac gccgaccaga
     3541 cccacggccg ctcgatggtg attgtcggcg cggcgctgaa ccactggtat cacatggaca
     3601 tgacctatcg cggcatcatc aatatgttga tgctgtgcgg ttgtattggt cagtccggcg
     3661 gcggctggtg tcattatgtc ggccaggaaa aactgcgccc gcaaaccggc tgggcgccgc
     3721 tggcgtttgc cgccgactgg aatcgcccgg cccggcagat gaacggcacc agcttcttct
     3781 acgcccacac cagccagtgg cggcatgaaa aactgggcgt gaatgaaatc ctctgcccga
     3841 ccgccgacgg caagatggcc aatatggcgc tgattgacta caacgccaag gccgagcgca
     3901 tgggctggct gccgtccgcc ccgcagctgg gcgccaaccc gctggacctc acccgccagg
     3961 ccgaagccgc cggcaaagac cccatcgcct acgccgtcga gcagctgaaa accggccagc
     4021 tggacatggc ctgcaacgac ccggacaacc cgcaaaactt cccgcgcaat ctgtttgtct
     4081 ggcgctcgaa tattctgggc agctcgggca aggggcatga gtatttcctg aaatacctgc
     4141 tgggcacgca aaacg
//
LOCUS       DAOPYX010000268         4145 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_28045_length_4145_cov_11.375306, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000268 DAOPYX010000000
VERSION     DAOPYX010000268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 4145)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4145)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4145
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28045_length_4145_cov_11.375306"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..682
                     /locus_tag="PKN55_11830"
     CDS             <1..682
                     /locus_tag="PKN55_11830"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNX71979.1"
                     /translation="SRLTESMPAMETTVLIADDHPLFREALRLVVAEALGAEAQTIEC
                     ASLDEAREQVAHSPNIDLALMDLNMPGMDGLAGLASLRAHMPTLPIIVVSADERHATV
                     DAALRAGVSGFIPKSFSRADMAAAVNAVLDGDIFFPATSDRNTGAPPAYDPGMTDMAA
                     RIATLTKQERCILEYLIRGKSNKFVAIELDIAESTVKAHVSSILRKLKVHSRTLAVIK
                     AGPLLQLN"
     gene            complement(821..1198)
                     /locus_tag="PKN55_11835"
     CDS             complement(821..1198)
                     /locus_tag="PKN55_11835"
                     /inference="COORDINATES: protein motif:HMM:NF016161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB2 family protein"
                     /protein_id="HNX71980.1"
                     /translation="MSYLALKHLHLTCAVLTALSFIARGLLLATRPQALNARFWRVAP
                     HVNDTLLLAAGVGMAVVGGFNPLTTPWLATKLVLLLAYIGLGTLALKRGKTPQQRAIA
                     FVLALAALAAIVHVALSKQGWWG"
     gene            1437..1934
                     /gene="tpx"
                     /locus_tag="PKN55_11840"
     CDS             1437..1934
                     /gene="tpx"
                     /locus_tag="PKN55_11840"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018746960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HNX71981.1"
                     /translation="MSVTLKGNPVEVRGEFPAVGTVAAEFSLVGADLADVSLSSLAGQ
                     RKVLNIFPSVDTPVCAASVRKFNAAAGSLANTVVLCISADLPFAQKRFCGAEGLEQVK
                     TLSTLRGGEFLANYGVAIASGPLAGLAARAVLVLDENNTVLYAERVAEITEEPNYGDA
                     LAVLG"
     gene            2035..3612
                     /locus_tag="PKN55_11845"
     CDS             2035..3612
                     /locus_tag="PKN55_11845"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNX71982.1"
                     /translation="MPSTPPPSPHIAQLLAQAEAAWNAGELERCEQLARQTLSQWPES
                     AMAWNLLGMVASRYGQLEQAAQLMTQAAKLDATQAQIRTNLAIVLSQLGELAQASALL
                     AQTLEFAPEHETAWQLLAQLLEMQGQFAEQADLLRQAHARCPGSLTLQLMLSDALNTM
                     GEHVQAAKLAADVTHRYPKFADGWSCLGCAQEKLGQIDAARQSLQRAIALKPQEGQLH
                     VNLAHLELLVGNFADGWREYEWRFSCQQSAPLPNLAAPRWQGEPINGKVLLLWPEQGH
                     GDTLQFCRYAQLLAARGARVWLVCVPALWPLLAQGAPGIERLLPPEQAAAEAGQVDFW
                     APLLSLPHLTESGDDPLGYLTPYLHVEPQRQARWRARLDALPPGRRIGLIWSGNPLNG
                     NNPTRALPRAQLAALAGLDNVQWISLQMGVEGRQAPPEGLSWHAWGNEIADFADTAAI
                     VSQLDLLISTDTGPAHLAGALGAPTWLLVQRVPDWRWGLNGEQCASYPSMRLFRQREQ
                     GDWASVMRTVRAALLAG"
     gene            complement(3705..>4145)
                     /locus_tag="PKN55_11850"
     CDS             complement(3705..>4145)
                     /locus_tag="PKN55_11850"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNX71983.1"
                     /translation="FAVVADEVRKLAERSSQSTEEINAAVAKIGEVVEQATRNMDETV
                     EEVQRGAAEIESASDNLAQLIMAADEASTQAEQLAGVSQQQSQATHQIAATLQQVSVA
                     TEEHMQATEALSEASAQLAQTASDLEKLVGHFSRWHTPAADHDD"
BASE COUNT          702 a         1332 c         1341 g          770 t
ORIGIN      
        1 ttcacgcctg accgaaagta tgcccgccat ggaaaccact gtcctgattg ccgatgatca
       61 tccgctgttt cgcgaagccc tgcgcctggt ggtcgccgaa gcgctgggcg ccgaggcgca
      121 aaccatcgag tgcgccagcc tggacgaggc acgcgagcaa gtggcgcaca gcccgaatat
      181 tgatctggcc ttgatggact tgaacatgcc gggcatggat ggcctggccg ggttggccag
      241 cttgcgcgcg cacatgccca ccctgcccat catcgtggtg tccgccgacg agcgccacgc
      301 cacggtggac gccgcgctgc gcgccggcgt gtcgggcttt atccccaagt cgttctcacg
      361 cgccgacatg gccgcggcgg tcaacgcggt gctggacggc gatattttct ttcccgccac
      421 cagcgaccgc aacaccggcg cgccgcccgc ctacgacccg ggcatgaccg acatggccgc
      481 gcgcatcgcc acgctgacca agcaagagcg ctgcattctg gaatacctga tccgtggcaa
      541 gtccaataaa tttgtcgcca tcgagctgga tatcgccgaa agcacggtca aggcgcatgt
      601 gtcgtcgatt ctgcgcaaac tcaaggtgca cagccgcacg ctggcggtga tcaaggccgg
      661 gccgttgttg cagctgaact gagcgccgct cagcaaactc tcctggcgtc attgcgcggc
      721 cttgccgtgc cgctacgctg agtgtggtga gcgatgcgaa gcaccgctca tccccccctg
      781 gcagcctgct tttttcctgc caagggaagg catgcgcgat tcagccccac cagccctgct
      841 tgctcaaagc cacatgcaca atcgccgcca gcgccgccaa ggccagcaca aaggcaatcg
      901 cccgttgttg cggggttttg ccgcgcttga gcgccagcgt gcccaggcca atataggcca
      961 gcagcagcac cagcttggtg gccagccagg gcgtggtcag cgggttgaat ccaccgacca
     1021 ccgccatgcc caccccggcc gccagcagca aggtgtcgtt gacatgcggg gccacacgcc
     1081 agaagcgcgc attcagcgcc tgtggccggg tggccagcag caggccacgg gcaataaaag
     1141 acagcgcggt cagcaccgcg caggtgagat gcagatgttt cagggcaagg tagctcatgg
     1201 ctggatcata ccgccgccca caggggtgag ccactgaata aaacccctgt tttcaacagg
     1261 gttgcatttc cggtgcaatc tagcacactg atggcatgtt gccgtgttta gcgccgctca
     1321 ccaaactcag cgcagcaatc ctcgctaggc gcgcgcagac caaggccgcg caacgatgcc
     1381 aggcgggttt ggtgagcggc gcttacggca ttatccaccc ccatggagca gatcacatga
     1441 gtgttaccct gaaaggcaat ccggttgaag tgcgtggcga gtttcccgcg gtgggcacgg
     1501 tcgcggctga gttttctctg gtcggcgccg acctggccga cgtgtcgttg tcctcgctgg
     1561 ccggccagcg caaagtgctg aatattttcc cgagcgtgga caccccggtg tgcgccgcct
     1621 cggtgcgcaa gttcaacgct gccgccggca gcctggccaa caccgtggtg ttgtgcattt
     1681 ctgccgacct gccgtttgcg caaaagcgct tttgcggcgc cgaaggcctg gagcaggtga
     1741 aaaccctgtc caccctgcgc ggcggtgaat tcctggccaa ctacggtgtg gccatcgcca
     1801 gcggcccgct ggccggcctg gccgcccgcg ccgtgctggt gctggatgaa aacaacaccg
     1861 tgctgtacgc cgagcgcgtg gcggaaatca ccgaagagcc gaactacggc gacgcactgg
     1921 ccgtgctggg ctgagccccc gtctgatgat tctggccggg tgttgacccg tccggttgtc
     1981 atcccctcct gaagcggcta tggtgctggc tttcccttgt agcggacagc cagcatgccc
     2041 tccacgccgc ccccttctcc ccacattgct caattgctcg cccaagccga agccgcctgg
     2101 aatgccggcg agctggaacg ctgcgagcaa ctggcgcggc aaaccttgtc gcagtggccc
     2161 gagtcagcga tggcctggaa tctgctgggc atggtggcca gccgctatgg ccaactggag
     2221 caggccgctc agctgatgac tcaggcagcc aagctggacg ccactcaggc acaaatccgc
     2281 accaatctgg ccattgtgct cagccaactg ggcgagctgg cccaggccag tgcgctgctg
     2341 gcgcaaacgc tggaatttgc tcccgaacac gaaaccgcct ggcagctgct ggcgcaattg
     2401 ctggaaatgc agggtcagtt tgccgagcaa gccgatttgc tgcgccaggc gcacgcgcgt
     2461 tgccctggct cgctgacttt gcagctaatg ctgagcgatg cgctgaacac catgggtgag
     2521 catgtccagg ccgccaagct ggcggctgat gtgactcacc gctaccccaa gttcgccgat
     2581 ggctggtcct gcctgggttg cgcgcaggag aaactcggcc agatcgacgc cgcccggcag
     2641 tctttgcaac gggccattgc gctgaagcca caagagggtc agttgcatgt gaatctggcg
     2701 catctggagc tgctggtggg taattttgcc gatggttggc gtgaatacga atggcgcttt
     2761 agctgccaac aatcggcacc cttgccaaat ttggcggcgc cgcgctggca aggggagccg
     2821 attaacggca aggtgctgct gttatggcca gagcaaggcc atggcgacac cttgcagttt
     2881 tgccgctatg cccagttgct ggcggcgcgc ggcgcgcggg tgtggctggt gtgtgtgccg
     2941 gccttgtggc cgctgctggc gcagggtgcg cccggcattg agcgcttgtt gccgccagag
     3001 caggcggcgg cagaagccgg gcaggtggat ttttgggcgc ctttgctgag cctgccgcac
     3061 ctgaccgaat ccggcgacga cccattgggt tatttgacgc cttatttaca cgttgagcca
     3121 cagcggcagg cgcgctggcg ggcgcggctg gatgcactgc cgccaggccg gcgcattggc
     3181 ctgatctgga gcggcaatcc gctgaacggc aataacccca cccgcgcctt gccgcgcgcg
     3241 cagttggcgg cgctggccgg gctggacaat gtgcaatgga tcagcctgca aatgggcgtg
     3301 gaaggccgcc aagcgccgcc cgaagggctc agctggcacg cctggggcaa tgaaattgct
     3361 gattttgccg acaccgccgc gattgtcagt cagttggact tgctgatcag caccgacacc
     3421 ggccctgccc acctggccgg cgcgctgggc gcgcccacct ggctgctggt gcagcgcgtg
     3481 ccggactggc gctggggctt gaacggcgag cagtgcgcca gctaccccag catgcggctg
     3541 ttccgccagc gcgagcaggg cgattgggcg tcggtgatgc gtacggtgcg cgcggcgctg
     3601 ctggctggct gagcgtgact cacccgcgcg cttagctttg ccctgcgtct gccgccatta
     3661 gccaggcgcg cttacccagc atgcagagcg cttccgcgcc gcgctcagtc gtcgtgatcg
     3721 gcggcaggag tgtgccagcg tgagaagtgg ccgaccagtt tttccaggtc gctggcggtt
     3781 tgcgccagct gcgccgaggc ttcggacaag gcctcggtgg cttgcatatg ctcttcggtg
     3841 gccaccgaca cctgttgcaa ggtggcggca atctggtggg tggcctgcga ttgctgctga
     3901 ctgacgccgg ccagttgttc ggcctgggtg ctggcttcgt cggcagccat gatcagttgg
     3961 gccagattat cgctggcgga ttcgatttca gcggcgccgc gctgcacctc ttccacggtt
     4021 tcgtccatat tgcgcgtagc ctgctccacc acttcgccaa tcttggccac ggcggcgttg
     4081 atttcttcgg tggattgact ggagcgctcg gccagcttgc gcacttcatc ggccaccacg
     4141 gcaaa
//
LOCUS       DAOPYX010000269         4126 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_28245_length_4126_cov_7.261852, whole genome shotgun sequence.
ACCESSION   DAOPYX010000269 DAOPYX010000000
VERSION     DAOPYX010000269.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 4126)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4126)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4126
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28245_length_4126_cov_7.261852"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..316)
                     /locus_tag="PKN55_11855"
     CDS             complement(<1..316)
                     /locus_tag="PKN55_11855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018749472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HNX71984.1"
                     /translation="MKYVDEFRDGHAARALAAEIAAVVQPDRQYRLMEFCGGHTHAIA
                     RYGLSALLPDTVELIHGPGCPVCVLPIGRLDGAIALALEHGVTLCTYGDTLRVPGSGR
                     MSL"
     gene            complement(313..537)
                     /locus_tag="PKN55_11860"
     CDS             complement(313..537)
                     /locus_tag="PKN55_11860"
                     /inference="COORDINATES: protein motif:HMM:TIGR00074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HypC/HybG/HupF family hydrogenase formation
                     chaperone"
                     /protein_id="HNX71985.1"
                     /translation="MPVQVTELLPGQRARVSVDGVSREVSVALVEDLAVGDFVILHVG
                     YAIGKLDEDEARRTLALIAEAVDKLAEGAA"
     gene            complement(751..2874)
                     /locus_tag="PKN55_11865"
     CDS             complement(751..2874)
                     /locus_tag="PKN55_11865"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNX71986.1"
                     /translation="MRHSPHALSLTVGALLAALLPAAQAAEPTEPDAAVLSEITVSAT
                     RSAEALRRDASAGKLVVGRQELDALNSSSVAELLRKLPGAGIFTDLDNPGRGGRSRVA
                     ERNLPQILVDGQLLPGGGRAPATALRLPVELIERVEIIRNSTAEFPNVSPAGTINLIL
                     RDAPNRRTLAAKLGVGASQGRPSLRAEGQYGNREGGFSYLLSGSLTERPSQGSRDTAR
                     SLYSAQGETRQQEAAHSHGQDSNASFSPRFTWRLGEYGQLIVSPLLSYTRADQRSSTD
                     IPGVSHEDSQQHDTRASARLLTEWKLSHADNGETSIKLMSQGEREQSRRRSVRPDASG
                     AGQSQQDDTLRHEREWLASARHQRALGEAHLLTGALEWRIRSGDDRQNGGRDPSLAGQ
                     QAETRERQQVFWIQDEWQLAGQQVLTPGLRWQLDHTAVSDMDGERRRSHRAWLPSLHY
                     LWQVNSQWNLRASASRNNRAPSIYELSPVVRAASGDNSASNPDRGGNPDLRGEQLTVL
                     DMGIEHFLPDRAGVIGLSLFARRIDNYSQRLTLLEGERWVARPHNLGQASTRGLLFDA
                     KAKLSQWGLPQLTVRSNAMLSRTRLRDSPLGDGPRKSANLGLDYESPAGWSVGGTLSH
                     VSTLDRDSSLTVRQQQDANTQLDLYATYKLDRQLTARLALSNVTGAKSRNQVWEYDSS
                     GALQRQEVESRRANRSVLLTLEGKW"
     gene            complement(3017..>4126)
                     /locus_tag="PKN55_11870"
     CDS             complement(3017..>4126)
                     /locus_tag="PKN55_11870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008952860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HNX71987.1"
                     /translation="CAPITWPIGMGKTFRGVYHLMNDQVMLFEAGQDIADQLVSEVIQ
                     GIDNPRLDELFPLEMAQLRMEIELVKGASNEFDLEAFLAGELTPVFFGSAINNFGVRE
                     ILNALVDWAPPPSPRDASVRMVDPSEEKFSGFVFKIQANMDPKHRDRIAFMRVCSGRF
                     ERGMKMKHLRLNRDIAASSVVTFMAHDREIVEEAFAGDIIGIPNHGNIQIGDSFSEGE
                     ALQFTGIPFFAPELFRSVRIRNPLKIKQLHKGLQQLGEEGAVQVFKPANGADLILGAV
                     GVLQFEVVAHRLQTEYGVEAVFDPCQIYTARWVSCDDARMLNDFEKALVNNVAIDAAG
                     SLAYLAPNRVNLDLTQERWPKVVFHETREHAVKLS"
BASE COUNT          659 a         1340 c         1360 g          767 t
ORIGIN      
        1 acaagctcat gcgcccggaa ccgggcacgc gcagggtgtc gccgtacgtg cacagggtca
       61 cgccgtgttc cagcgccagg gcaatcgccc catccagccg gccaatcggc agcacacaca
      121 ccggacagcc ggggccgtgg atcagctcga cagtgtccgg cagcaaggcg ctcagcccgt
      181 agcgggcaat ggcgtgggtg tggccgccgc aaaactccat cagccgatac tgacggtcgg
      241 gctggaccac ggcggcaatc tcggccgcca gcgcgcgcgc ggcgtggcca tcgcggaatt
      301 cgtccacgta tttcatgccg cgccctccgc cagcttgtcc accgcctcgg caatcaaggc
      361 cagcgtgcgc cgcgcctcgt cctcgtccag cttgccgatg gcgtagccga cgtgcaggat
      421 gacaaaatcc cccacggcca ggtcttccac cagcgccacc gacacctcac ggctgacgcc
      481 atccacgctg acgcgcgcgc gctgaccggg caacagctcg gtcacttgaa ctggcatggc
      541 cagacacatg cttgtgactc cttacaaaat gtcttggcaa aaatcgccag gacgaaacat
      601 ccatgggtgc gctgtgcgcc agtcacgcac gacgtggaac cgtctgggct cccgcctgcg
      661 cgtatacatt ttgatttttc aggggctgac tttgggcaaa ttttatccct ataggctgaa
      721 tccatccgcg catttctcac gcccccgggt tcaccacttg ccttccagcg tcagcagcac
      781 actgcggttg gcgcggcggc tttcgacttc ctggcgctgc aaagcgccgc tgctgtcgta
      841 ttcccacacc tgattgcgtg attttgcccc ggtgacattg ctcaaagcca gccgcgccgt
      901 caactgacga tccagcttat aggtggcgta caggtccagc tgggtgttgg cgtcttgctg
      961 ctggcgcacg gtcaggctgc tgtcgcggtc cagcgtggac acatggctga gcgtgccgcc
     1021 cacgctccag ccggcggggg attcgtaatc cagccccagg ttggcgcttt tgcgcgggcc
     1081 gtcgcccagt gggctgtcgc gcaggcgggt gcgcgacagc atcgcattgc tgcgcacggt
     1141 aagctgcggc aggccccatt ggctgagttt ggccttggcg tcgaacagca ggccgcgggt
     1201 gctggcctgg ccgagattat gcgggcgcgc cacccagcgc tcgccttcca gcagcgtcag
     1261 ccgctggctg tagttgtcga tgcggcgggc aaacaaggac aggccaatca ccccggcgcg
     1321 gtcgggcaaa aagtgctcga tgcccatgtc cagcacggtg agctgttcgc cgcgcaaatc
     1381 cgggttgccg ccccggtccg ggttgctggc gctgttgtcg ccgctggcag cgcgcaccac
     1441 cgggctgagc tcataaatcg acggcgcgcg gttattgcgg ctggcgctgg cgcgcagatt
     1501 ccattggctg ttcacctgcc acagataatg caaggacggc aaccaggcac gatggctgcg
     1561 ccgacgctcg ccgtccatat cgctgaccgc ggtatggtcc agctgccagc gcaggccggg
     1621 ggtgagcacc tgttgcccgg ccagttgcca ttcgtcctga atccagaaca cctgctggcg
     1681 ttcgcgggtt tctgcctgct ggccggccag actggggtcg cgtccgccgt tttggcggtc
     1741 gtcgccgctg cggatgcgcc attccagcgc gccggtcagc agatgcgcct cgcccaatgc
     1801 gcgctgatgg cgggcgctgg ccagccattc gcgctcgtgg cgcagcgtgt cgtcttgctg
     1861 gctttgcccg gcgccgctgg cgtctggccg cacgctgcgt cggcgcgatt gctcgcgctc
     1921 gccctggctc atcaacttga tgctggtttc gccattgtcg gcgtggctga gcttccactc
     1981 ggtcagcagg cgggcgctgg cgcgggtgtc gtgctgctgg ctgtcttcgt ggctgacgcc
     2041 ggggatgtcg gtgctgctgc gctggtcggc gcgggtgtag ctgagcaagg ggctgacaat
     2101 caactggcca tactcgccca gccgccacgt aaagcgcggg ctgaagctgg cgttgctatc
     2161 ctggccatgg ctgtgcgcgg cttcttgctg ccgggtttcg ccctgggcgc tgtacaggct
     2221 gcgcgcggtg tcgcggctgc cttggctggg gcgctcggtg agcgagccgg acagcaaata
     2281 actgaacccg ccctcccgat tgccatattg cccttcggcg cgcaggctgg gccggccctg
     2341 gctggcgccc acgcccagct tggccgccag cgtgcgccgg ttgggcgcgt cgcgcagaat
     2401 cagattgatg gtgccagccg ggctgacatt ggggaattcc gccgtgctgt tgcggatgat
     2461 ctccacccgc tcgatcagct ccaccggcag gcgcagcgcg gtggccggcg cgcggccgcc
     2521 gccgggcagc aactggccat ccaccagaat ttgcggcaga ttgcgttcgg caacgcggct
     2581 gcgcccgccc cggccggggt tgtccagatc ggtaaaaatc ccggcgccgg gcagcttgcg
     2641 caatagctcg gccacgctgc tgctgttgag cgcgtccagc tcctggcggc cgaccaccag
     2701 cttgccggcg ctggcgtcgc ggcgcagggc ttcggcgctg cgggtggcgc tgacggtgat
     2761 ttccgacaac acggcggcgt cgggctcggt gggttcggca gcctgggcgg caggcagcag
     2821 ggcggccagc aaggcgccaa cggtcaggct gagggcgtgg ggagaatggc gcatggcgga
     2881 catttcagca ggagggcggg cggccaggcc ggcatatccg gcctgaccac acaggcttgg
     2941 cgtggctcac aaaacccagc gtagcggccc tacggcaagg tcacgccaca aggccagggg
     3001 gttttgtcag cggcgcttac gacagtttca ccgcgtgttc gcgggtttcg tggaacacca
     3061 ccttgggcca gcgctcctgg gtgaggtcca gattgacgcg gttgggcgcc aggtaggcca
     3121 ggctgccggc ggcgtcgatg gcgacgttgt tgaccagggc tttttcaaag tcgttgagca
     3181 tgcgcgcgtc gtcgcagctc acccagcggg cggtgtaaat ctggcagggg tcgaacaccg
     3241 cttccacgcc gtattcggtt tgcagacggt gcgccaccac ttcaaattgc agcacgccca
     3301 ccgcgcccag aatcaaatcg gcgccattgg ccggcttgaa cacctgcacc gcgccttctt
     3361 cgcccagctg ttgcaagccc ttgtgcaatt gcttgatttt cagcgggttg cggatgcgca
     3421 ccgagcggaa cagctccggg gcaaaaaacg gaatgccggt aaattgcagc gcctcgcctt
     3481 ccgagaagct gtcgccaatc tggatattgc cgtggttggg aatgccaatg atgtcgccgg
     3541 caaaggcttc ttcgacaatc tcgcggtcgt gggccatgaa ggtcaccacg ctggaggcgg
     3601 cgatgtcgcg gttcagccgc aggtgcttca ttttcatgcc gcgctcaaaa cggccggaac
     3661 acacgcgcat aaaagcaatg cggtcgcggt gtttcgggtc catattggct tggatcttga
     3721 acacaaagcc ggagaatttt tcttcgctcg ggtccaccat gcgcacgctg gcatcgcgcg
     3781 gcgacggcgg cggcgcccag tccaccaggg cgttgaggat ttcgcgcacg ccaaagttat
     3841 tgatggccga gccaaaaaac accggggtca gctcgccggc caggaaggct tccaggtcga
     3901 actcattgga tgcgcccttg accagctcga tttccatgcg cagctgggcc atttccagcg
     3961 ggaacagctc gtccagacgc gggttgtcga tgccctgaat cacctcgctg accaattggt
     4021 cggcgatgtc ttgcccggct tcaaacagca tcacctggtc attcatcagg tgatacacgc
     4081 cacggaaggt cttgcccatg ccaatcggcc aggtgatcgg cgcgca
//
LOCUS       DAOPYX010000270         4052 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_29004_length_4052_cov_8.297473, whole genome shotgun sequence.
ACCESSION   DAOPYX010000270 DAOPYX010000000
VERSION     DAOPYX010000270.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 4052)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4052)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4052
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29004_length_4052_cov_8.297473"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..200
                     /locus_tag="PKN55_11875"
     CDS             <1..200
                     /locus_tag="PKN55_11875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX71988.1"
                     /translation="ALLPDLACAGREKNKNNRLKRLIYTWRSDVKPSGGQATRPWAEM
                     HTGFQPGAHRQSQGLRLFRKC"
     gene            217..2595
                     /locus_tag="PKN55_11880"
     CDS             217..2595
                     /locus_tag="PKN55_11880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008953594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase/enoyl-CoA
                     hydratase family protein"
                     /protein_id="HNX71989.1"
                     /translation="MSQAKFNVRKVAVLGAGVMGAQIAAHLVNAKVQTVLFDLPAKEG
                     DKSSIALKAIANLKKLKPAPLAAADRADYITPANYEEHLHLLKDCDLVIEAIAERMDW
                     KADLYHKVAPHLGENSIFATNTSGLSINTLAQACPDSLRPRFCGVHFFNPPRYMHLVE
                     IIPCLTSDAGVLDNLERFLVTTLGKGVVRAKDTPNFVANRIGVFSMLATIANAEKYGI
                     RFDVVDDLTGPRLGRPKSATFRTADVVGLDTFAHVVKTMQDTLPNDPWHGLFKTPAWL
                     AALIEKGALGQKTKCGIYKKEGKQTLVLDPAKGEYVNAGEKGDDAVKAILKETNPAVK
                     FKKLRDSQHPQAQFLWACFRDVFHYIAYHLADVADNARDLDLAIRWGFGWSVGPFETW
                     QAAGWQQVAGWMQEDIAAGHSLSQAALPAWALEADRAGVHFAHGSYNAAQGTLAGRST
                     LDVYQRQLAPARVLGEDAPQLGETVFETEGGVRAFTSGDGVLVVSFTSKAHAIGPAVI
                     EGLNKAIDLAEAQYKALVIWQTEEPFSVGADLQSMMPAFMTGNWAAIDDTVRQFQNTS
                     MRLRYSLVPVVAASQGYAFGGGCEFLMHCDKVVAALETYIGLVEVGVGLLPGGGGCKE
                     FALRAAQEARGDVFAALKDYFMAIATAKVGTSALEGQAIGYLKKTDTVVFNVHELLYV
                     AKQEALAMAEAGYRPPLKAKGFPVAGRSGAASIKGQLVNMLEGNFITKHDFTIASLIA
                     DVMTGGDLEAGTLVDEQWLLDLERKAFMSLLKNPLSQARIANMLTTGKPLRN"
     gene            2651..3853
                     /locus_tag="PKN55_11885"
     CDS             2651..3853
                     /locus_tag="PKN55_11885"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012697082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HNX71990.1"
                     /translation="MSKQIQEAYIVATTRTPVGKAPRGMLRHVRPDDMLAHVLKGALA
                     QVPELDVNLINDVVVGCAFPEAEQGLNMARIGALLAGLPETVGGLTINRYCSSGINAV
                     QIAADRIRTGEADVVIAAGSESMSMVPMMGNKVSLNPAIFAKDENYAIAYGMGLTAEK
                     VAQQWKVSREDQDAFAVESHRRAIAAIQSGAFANEITPLDATYRTPNLATGEVILRNK
                     VLDTDEGPRLDTTLEGLAKLKTVFDAKGSVTAGNSSQMSDGAGAVVLVSEKVLKQFNL
                     TPLARYVSFAVKGVPPAIMGIGPKVAIPAALANAGIAQSDLKWIELNEAFAAQALAVM
                     RDLELDPAVVNPNGGAIALGHPLGATGAIRTATLVHGMRRRGQTGYGMVTMCIGTGMG
                     AAGILEIL"
BASE COUNT          837 a         1367 c         1215 g          633 t
ORIGIN      
        1 cagcgcttct tcccgacctg gcttgcgccg gtcgggaaaa aaataaaaac aaccgtttaa
       61 aacgtttgat ttatacttgg cgctctgacg tcaaaccatc gggtggccag gccacccggc
      121 cgtgggcgga gatgcataca ggattccaac cgggcgcaca ccggcaatcg cagggactcc
      181 gcctgtttcg caagtgctga ccaaactgag gtaaccatgt ctcaagcgaa attcaacgta
      241 cgcaaggtgg ccgtgctcgg cgccggtgtg atgggggcgc aaatcgccgc ccacctggtc
      301 aacgccaagg tgcaaaccgt gttgttcgac ctcccggcca aagaaggcga caaaagcagc
      361 atcgcgctga aagccatcgc caacctgaaa aagctcaagc cggccccgct ggccgccgct
      421 gaccgcgccg actacatcac cccggccaac tacgaagaac acctgcactt gctcaaagac
      481 tgcgacctgg tgattgaagc catcgccgaa cgcatggact ggaaagccga cctgtaccac
      541 aaggtggcgc cgcacctggg cgaaaacagc atttttgcca ccaacacctc gggcctgagc
      601 atcaacaccc tggcccaggc ctgcccggac agcctgcgcc cgcgcttttg cggcgtgcac
      661 tttttcaacc cgccgcgcta tatgcacctg gtggaaatca tcccctgcct gaccagcgac
      721 gctggcgtgc tggacaacct ggaacgcttc ctggtcacca ccctgggcaa gggcgtggtg
      781 cgcgccaaag acaccccgaa ctttgtcgcc aaccgcatcg gcgtgttctc catgctggcc
      841 accatcgcca atgccgaaaa atacggcatc cgctttgacg tggtggacga cctcaccggc
      901 ccgcgcctgg gccgcccgaa atccgccacc ttccgcaccg ccgacgtggt gggcctggac
      961 accttcgccc acgtggtcaa gaccatgcag gacaccctgc ccaacgaccc gtggcacggc
     1021 ctgttcaaaa ccccggcctg gctggccgcg ctgattgaaa aaggcgcgct ggggcaaaaa
     1081 accaagtgcg gcatctacaa aaaagaaggc aagcaaaccc tggtgctcga cccggccaag
     1141 ggtgaatacg tcaatgccgg cgaaaaaggc gacgacgcgg tcaaggctat cctgaaagaa
     1201 accaacccgg cggtgaaatt caaaaagctg cgcgacagcc agcacccgca ggcgcagttc
     1261 ctgtgggcgt gctttcgcga cgtgttccac tacatcgcct accacctggc cgatgtggcc
     1321 gacaacgccc gcgacctcga cctggccatc cgctggggct ttggctggtc ggttggcccg
     1381 tttgaaacct ggcaagccgc cggctggcag caagtggccg gctggatgca agaagacatc
     1441 gccgctggcc acagcctgag ccaggccgcg ctgccggcct gggcgctgga agccgaccgc
     1501 gcaggcgtgc actttgccca cggctcgtac aacgccgccc aaggcacact ggccggccgc
     1561 tccaccctgg acgtttacca gcgccagctg gccccggccc gcgtgctggg cgaagacgcg
     1621 ccgcaactgg gcgaaaccgt gttcgaaacc gaaggcggcg tgcgcgcctt cacctcgggc
     1681 gacggcgtgc tggtggtgag cttcacctcc aaggcccacg ccattggccc ggcagtgatc
     1741 gaagggctga acaaggccat cgacctggcc gaagcgcaat ataaagccct ggtgatctgg
     1801 caaaccgaag aaccgttctc ggtgggcgcc gacctgcaat cgatgatgcc ggccttcatg
     1861 accggcaact gggcggcgat tgacgacacc gtgcgtcagt tccagaacac ctcgatgcgt
     1921 ctgcgctaca gcctggtgcc ggtggtggcg gcctcgcaag gctacgcctt tggcggcggc
     1981 tgcgagttcc tgatgcactg cgacaaggtg gtggccgcgc tggaaaccta tatcggcctg
     2041 gtggaagtgg gcgttggcct gctgccgggt ggcggcggct gcaaggaatt tgccctgcgc
     2101 gccgcgcagg aagcccgtgg cgatgtattt gccgcgctga aagactactt catggccatt
     2161 gccaccgcca aggtgggcac cagcgcgctg gaaggccagg ccatcggcta cctgaaaaag
     2221 accgacaccg tggtgttcaa tgtgcacgag ctgctgtacg tggccaagca agaagcgctg
     2281 gccatggccg aagccggcta tcgcccgccg ctgaaagcca agggcttccc ggtggccggc
     2341 cgcagcggcg ccgcttcgat caagggccag ctggtgaaca tgctggaagg caatttcatc
     2401 accaagcatg acttcaccat cgccagcctg atcgccgacg tgatgaccgg cggcgacctg
     2461 gaagccggca cgctggtgga cgaacaatgg ctgctggatc tggagcgcaa ggccttcatg
     2521 agcctgctga aaaacccgct gagccaggcg cgcatcgcca atatgctgac cactggcaag
     2581 ccgctgcgca actgatcgcc cgaacccttg cggcgcgcgc ccagccggcg cgcgcctgga
     2641 gaatgacctc atgagcaaac agattcaaga agcctatatc gtcgccacca cccgcacccc
     2701 ggtgggcaaa gccccgcgcg gcatgctgcg ccacgtccgc ccggacgaca tgctggccca
     2761 cgtgctcaag ggcgcgctgg cgcaagtgcc ggaactggac gtcaacctga tcaacgatgt
     2821 ggtggtgggc tgcgccttcc ccgaagccga gcaaggcctg aacatggccc gcatcggcgc
     2881 cctgctggcc ggcctgccgg aaaccgtcgg cggcctcacc atcaaccgct actgctcgtc
     2941 cggcatcaac gccgtgcaaa tcgccgccga ccgcatccgc actggcgaag ccgacgtggt
     3001 gattgccgcc ggttcggaat ccatgtccat ggtgccgatg atgggcaaca aggtgtcgct
     3061 gaacccggcc atttttgcca aagacgaaaa ctacgccatc gcctacggca tgggcctgac
     3121 cgcagaaaaa gtcgcccagc agtggaaagt cagccgcgaa gaccaggacg cctttgccgt
     3181 ggaatcccac cgccgcgcca tcgccgccat ccagtctggc gcctttgcca atgaaatcac
     3241 cccgctggac gccacctacc gcacgcccaa cctggccacc ggcgaagtga ttctgcgcaa
     3301 taaagtgctg gacaccgacg aaggcccgcg cctggacacc acgctggaag gcctggccaa
     3361 gctgaaaacc gtgttcgacg ccaagggcag cgtcaccgcc ggcaacagct cgcaaatgtc
     3421 cgacggcgct ggcgccgtgg tgctggtcag cgaaaaggtg ctcaagcagt tcaacctcac
     3481 cccgctggcc cgctatgtga gctttgccgt caaaggcgtg ccgccggcca tcatgggcat
     3541 cggcccgaaa gtggccatcc ccgccgccct ggccaacgcc ggcatcgccc aatccgacct
     3601 gaaatggatt gagctgaacg aagcctttgc cgcccaggcg ctggcggtga tgcgcgacct
     3661 ggagctggac ccggccgtgg tcaaccccaa cggcggcgcc atcgccctgg gccacccgct
     3721 gggcgccacc ggcgccatcc gcaccgccac cctggtgcac ggcatgcgcc gccgtggcca
     3781 gaccggctat ggcatggtca ccatgtgcat cggcaccggc atgggcgctg ccggcattct
     3841 ggaaatcctg taaacgctat ccgtaggcag gatgatgtaa aaacacaagc cgggcgatcc
     3901 ccggcttgtg tttttgtgat gcttcatgca atgcatgctt gcaaagcgac aaggcatgga
     3961 taaacacgtc gtgcagcgat cctggccaaa cgcaagtcat ctgcaaggct gcaccatcac
     4021 gtcaggcaga taaccctgtg ttcaggctgg cg
//
LOCUS       DAOPYX010000271         4021 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_29346_length_4021_cov_10.961422, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000271 DAOPYX010000000
VERSION     DAOPYX010000271.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 4021)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4021)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4021
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29346_length_4021_cov_10.961422"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(107..661)
                     /gene="greB"
                     /locus_tag="PKN55_11890"
     CDS             complement(107..661)
                     /gene="greB"
                     /locus_tag="PKN55_11890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008954942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003711 - transcription elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006354 - DNA-templated transcription
                     elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HNX71991.1"
                     /translation="MSKAFTREQDADDDDALPAERQLPKSSKNYLTPAGWQRMKDELY
                     HLVHRERPEIVNIVSWAASNGDRSENGDYLYGKKRMREIDRRIRFLTKRLEIAEVVHS
                     AGRDSEQIFFGATVTLLRGDGQEQTVAIVGVDEIDLPRNRISWISPLARTLLKAREGD
                     TVVLRGPEGDEDIEILEVQYLALD"
     gene            complement(687..1841)
                     /locus_tag="PKN55_11895"
     CDS             complement(687..1841)
                     /locus_tag="PKN55_11895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018151569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HNX71992.1"
                     /translation="MSKAKPSNAGGARQPVSRTRRDQPQAPRTPKGAQGRSLPRHPAD
                     EFTTRKPRTPLTGKPAPQGASATPGARRPVADGAQAQDGVSQSPRPPRAGKPSGPKVQ
                     RARKMAQRHGSQKAEKISRELRDKRVDVSHITEIRLQKALAQTGIGSRRDMEELIAAG
                     QVEVNGKVAELGCKITPDDKVRVQGKAVYIKWPDRLPRIIIYHKQEGELVTRDDPEGR
                     VTVFERLPQTRSSKWVAVGRLDLNTSGLLVLTTSGELANRMMHPSFEVEREYAVRTLG
                     ELTPEQMQQATRGVELDDGPAHFQHISEQGGEGANHWYKVILREGRNREVRRLFEHFG
                     LTVSRLIRVRFGSLHLPTRLKRGQFHELDEAEVLLVLKWAGLGLTGQQKP"
     gene            complement(1819..2403)
                     /gene="scpB"
                     /locus_tag="PKN55_11900"
     CDS             complement(1819..2403)
                     /gene="scpB"
                     /locus_tag="PKN55_11900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017510122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HNX71993.1"
                     /translation="MSTLTDLKYLKQVVETALLTAQEPLSVHDLKRLFTETLTNGLLY
                     EMVEEIQADWQGRGIELVKLASGWRFRARAEFQPYLARLNPEKPPRYSRAVMETVAII
                     AYRQPVTRGDIEAIRGVSVSSSVTQTLTERGWIEVIGHKEVPGRPGLYATTRKFLDDL
                     GLASLKDLPPLADLGSLVVPEKAEEPQDVESQTE"
     gene            complement(2536..3060)
                     /gene="ppa"
                     /locus_tag="PKN55_11905"
     CDS             complement(2536..3060)
                     /gene="ppa"
                     /locus_tag="PKN55_11905"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008954955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HNX71994.1"
                     /translation="MILDRVPAGKDIPNDFNVIIEIPAAGAPIKYELDKDTGAMFVDR
                     FMGTAMFYPANYGYVPQTLSEDGDPVDVLVVTPFPLQVGVVVRCRALGVLKMEDESGI
                     DAKLVAVPVEKLCPMYKDVQKLEDLPELLRAQISHFFEHYKDLEKGKWVKVQGWDGID
                     AAKQELLDGVARYK"
     gene            3189..3809
                     /gene="coq7"
                     /locus_tag="PKN55_11910"
     CDS             3189..3809
                     /gene="coq7"
                     /locus_tag="PKN55_11910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014088189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-polyprenyl-3-methyl-6-methoxy-1,4-benzoquinone
                     monooxygenase"
                     /protein_id="HNX71995.1"
                     /translation="MLDLLITEFDKGLRTLLAPAPTLRAYPDDGLEEAEMSDAERRHA
                     LGLMRINHCGEVCAQALYQGQALTARDPAAREALKQAAWEETEHLAWTERRIAELGGH
                     KSVFNPVWYAGSLAMGVTAGLLGDKWNLGFLEETEYQVEAHLNEHLESLPAQDAKSRA
                     IVSQMREDEVRHAETAHELGAAKLPLPVKGLMHVTSLVMKKSSYWV"
BASE COUNT          626 a         1192 c         1373 g          830 t
ORIGIN      
        1 caagcagtac ggcaaggtcg ggcaacgacg ccaggagggt tttgttagcc gctctaaaga
       61 taagctttcg ctgcgccggc cagatgatca tcaagggcgc gcccgttcag tccagcgcca
      121 ggtattggac ttccagaatc tcgatgtctt cgtcgccttc cgggccgcgc aacaccacgg
      181 tgtcgccttc gcgcgccttg agcagggtgc gcgccagcgg tgaaatccag ctgatgcggt
      241 tgcgcggcag gtcgatttcg tccacgccga caatcgccac ggtctgctcc tggccatcgc
      301 cgcgcagcag ggtgacggtg gcgccaaaaa agatttgctc gctgtcgcgg ccggcggaat
      361 gcaccacttc ggcgatttcc aggcgtttgg tcaaaaagcg gatgcgccgg tcgatctcgc
      421 gcatgcgctt cttgccgtag agataatcgc cattctccga gcggtcgccg ttggaggccg
      481 cccaggagac aatattgacg atctccgggc gctcgcggtg caccaggtga tacagctcat
      541 ccttcatgcg ctgccagccg gccggggtca ggtagttttt gctgcttttt ggcagttgcc
      601 gttcggcagg cagcgcatcg tcgtcgtcag cgtcttgttc gcgggtgaag gccttgctca
      661 tggtgggttc gcgccagagg gtagggtcag ggtttttgct ggccggtcag ccccaggcca
      721 gcccatttca gcaccagcag cacctcggct tcgtccagct cgtgaaactg gccgcgcttg
      781 aggcgggtgg gcaggtgcag gctgccaaag cgcacgcgga tcagccggct gacggtcagg
      841 ccaaagtgct cgaacaggcg gcgcacttcg cggttgcggc cttcgcgcag aatcaccttg
      901 taccagtggt tggcgccttc gccgccctgc tcggaaatat gctgaaagtg cgccgggccg
      961 tcgtccagct ccacgccgcg cgtggcctgc tgcatttgct cgggggtcag ctcgcccagc
     1021 gtgcgcacgg cgtattcgcg ctccacttca aagctggggt gcatcatgcg gttggccagc
     1081 tcgcccgagg tggtcagcac caaaaggccg ctggtgttca agtccagccg gcccaccgcc
     1141 acccatttgc tgctgcgcgt ttgcggcagg cgctcaaaca ccgtcacccg gccttccggg
     1201 tcgtcgcggg tgaccagctc gccttcttgc ttgtggtaga tgatgatgcg cggcaggcgg
     1261 tctggccatt tgatgtacac cgccttgcct tgcacgcgca ctttgtcgtc cggggtgatc
     1321 ttgcagccca gctcggccac cttgccgttg acttccactt gcccggcggc gatgagttct
     1381 tccatatcac ggcgcgagcc gatgccggtt tgcgccagcg ctttttgcag gcggatttcg
     1441 gtgatgtgcg agacatccac gcgcttgtcg cgcagctcgc gggagatttt ttcggctttt
     1501 tgcgagccat ggcgctgcgc catcttgcgc gcgcgctgca ccttggggcc agacggcttg
     1561 ccggcgcgcg gcggacgcgg gctctggctg acgccgtcct gagcctgggc gccgtcggcc
     1621 accgggcggc gggcgccagg cgtggcggac gcgccctgcg gggcgggctt gccggtcagc
     1681 ggggtgcgcg gcttgcgggt ggtgaattca tcggccggat ggcgcggcag gctgcggccc
     1741 tgcgcgccct tgggcgtgcg cggggcttgc ggttggtcgc ggcgggtgcg cgacaccggc
     1801 tggcgggcgc cgccagcgtt actcggtttg gctttcgaca tcttggggtt cttctgcttt
     1861 ctcggggaca accaggctgc ccagatcggc cagcggcggc aaatctttca ggctggccag
     1921 gcccaggtcg tccaggaatt tccgggtggt ggcgtacagg ccgggtcggc ctggcacttc
     1981 tttatggccg atgacttcaa tccagccgcg ttcggtcagc gtttgcgtca ccgacgacga
     2041 cacgctgacg ccgcgaatgg cttcgatatc gcctcgggtg accggctggc gataggcgat
     2101 gatggcgacg gtttccatca cggcgcgcga gtagcgcggc ggtttttccg ggttgagccg
     2161 ggccagatag ggctgaaact cggcgcgggc gcgaaaccgc cagcccgacg ccagcttgac
     2221 cagctcaatg ccgcgcccct gccagtcggc ctgaatctct tcgaccattt cgtacagcag
     2281 gccgttggtg agggtttcgg tgaacagccg cttgaggtcg tgcaccgaca gtggctcctg
     2341 cgcggtgagc agcgcagttt ccaccacctg tttcaggtat ttgaggtcgg tgagggtgct
     2401 catggtgtgt gtagtgtagc cggattttga ccgtgggttg taatgacgtg tgcgctggct
     2461 ttttgtttgc acggcgcgcc gcccaaacaa aaagccagcc ggcaggctgg cttttcaggc
     2521 tggctgtgcg ctgccttatt tataacgggc cacgccgtcg agcagctctt gcttggcggc
     2581 gtcgatgccg tcccagccct gaaccttcac ccacttgccc ttttccaggt ctttgtagtg
     2641 ctcgaagaag tggctgatct gggcgcgcag cagttccggc aggtcttcca gcttttgcac
     2701 gtccttgtac atcgggcaga gtttttccac cggcacggcc accagcttgg cgtcgatgcc
     2761 cgattcgtct tccatcttca gcacgcccag cgcgcggcag cgcaccacca cacccacttg
     2821 cagcgggaac ggggtgacca ccagcacgtc caccggatcg ccatcttccg acagggtttg
     2881 cggcacatag ccgtagttgg ccgggtagaa catggcggtg cccatgaaac ggtcaacgaa
     2941 catggcgcca gtgtccttgt ccagttcgta tttgatcgga gcgccggcgg ccgggatttc
     3001 gatgatgacg ttaaagtcgt tcgggatatc cttgccagcg gggacgcggt cgagaatcat
     3061 ggggctctcc agtcgtagta ttcgggttgt tatgggtcaa aatccggcca gattatataa
     3121 cagctcgccc accgcgcgca gggggcgggg gtataatcct ggcgtccttg tggagaaagt
     3181 gaaacaccat gctcgacctg ctgattaccg agttcgataa aggcctgcgc accttgctgg
     3241 cgccggcgcc caccttgcgc gcctacccgg atgacgggct ggaagaggcc gagatgagcg
     3301 acgccgagcg ccggcatgcg ctgggtttga tgcgcatcaa tcattgcggc gaggtgtgcg
     3361 cgcaggcctt gtatcagggc caggcgctga ctgcgcgcga cccggcggcg cgcgaggcgc
     3421 tcaagcaggc ggcgtgggag gaaaccgagc acctggcctg gaccgagcgg cgcattgccg
     3481 agttgggcgg gcataagagc gtgttcaatc cggtgtggta tgccggttcg ctggcgatgg
     3541 gggtgacggc gggtttgttg ggcgataagt ggaatctggg ttttctggaa gaaaccgagt
     3601 atcaggtgga ggcgcatctg aacgagcatc tggagagttt gccggcgcag gatgccaaga
     3661 gccgggcgat tgtcagtcag atgcgtgagg atgaggtgcg gcacgccgag acggcgcatg
     3721 agttgggggc ggcgaagttg cctttgccgg tgaagggctt gatgcatgtg acgtcgttgg
     3781 tgatgaagaa gagtagttat tgggtgtgag tgcttgtgta tgttgagccg cgctgtcgcg
     3841 cggggctgat tttgggcggg agttcgtccc gcagccgacc tactttcttt gcttcgccaa
     3901 agaaagtagg caaagaaagg cgaccccagg acgcccgccc gtccgccgcc tgcggcgacg
     3961 gtggttcctt gcgcttctcg aaaaccgggg ccgccacgga atggcgcgtc ggctgcgccg
     4021 c
//
LOCUS       DAOPYX010000272         4012 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_29447_length_4012_cov_6.679303, whole genome shotgun sequence.
ACCESSION   DAOPYX010000272 DAOPYX010000000
VERSION     DAOPYX010000272.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 4012)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4012)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4012
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29447_length_4012_cov_6.679303"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            127..1971
                     /locus_tag="PKN55_11915"
     CDS             127..1971
                     /locus_tag="PKN55_11915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014087647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HNX71996.1"
                     /translation="MFDFVQKNSLFIKVVLGGVALTFVGFGVGSYTAATDDPYLAKVD
                     GVKIYKQDIERALNGRPADAATRQGVLENLVRKELLLADARSSGLVVSADELRRAILD
                     IPGLQENGKFSKTQYELFLKSQGLSAPHFEKMIGDDLLIQKQLDSIAGSSLVSNAGIE
                     RVHQLIGQSREISAWVLRPQQFATEVKIDDEAVKAYYNAQSKRYQTPDMVRAQYVVLS
                     QDKLAASIKVSDEEIRQYFEQHRAELAPEQRRVAHILIAAGKDADAAARTKAREQAEA
                     LLKELKTNPGRFAELARARSQDPGSAAQGGDLGYFAKGMMVKPFEDTAFKLAKGEMSG
                     VVETDFGYHILRLDDIKQPDFAAVKEQVTARLQKQKATGALREQAEKLNELAYQQADS
                     LKAASDTLKLPLETSDWLARDSKPTNPVLAHPKLLDALFSDDVLKGKHNSEAIEVAPG
                     TLVVVRVAEHKPAAQKPLEEVAASIRQTLTDSKAAELASEKGKTLLATLKAGKAAAGE
                     SWGPMQTVSRQAPVSLSRDAARVVFSAPVAKLPAYAGASTDTGDYVIYRISKVTPAPA
                     AKPEDQARLRQMLSQAMSNAEAMAYVESLRQRFKVVQRPITGGEDSQQ"
     gene            complement(2070..3878)
                     /locus_tag="PKN55_11920"
     CDS             complement(2070..3878)
                     /locus_tag="PKN55_11920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012697074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNX71997.1"
                     /translation="MNWFWQTYTLVEKTFWNSLSRKLCSFFFISLFQLGFVALLWFYF
                     GEVQHVLEAAQLKPDVVQTLQNTMNRALQAVVALWFVSLAFIAFMVWYLRFLIVRPLR
                     MIISIFNEIGQGEGDLSRDIPAMTFDEIRELSESYNRFLRKMREIISNVRLMTVRIAM
                     DSARSAVNINDSLGSARKQGEFAREVRQSSDATTAGISQVSEQTQDISYTTQDNLAVA
                     KSSCAELLDVTARIEAVSQKVNRFNQTVEGLNLRSASIKQIVDLIKDISDQTNLLALN
                     AAIEAARAGEAGRGFAVVADEVRKLAERVKVATDEISENIDGMLRLVGNTLDETGQIT
                     SDTRLASETVSKATQHFEKMVVDYDNTARSLTDIARTMEQFAATNKQVNRHVSDIDEL
                     SQQVTDKLQQSHKVSQELSHASEQVQELVSRFSVGEGDFDHAINRARSYREMLEKRLQ
                     EMADRGVNLFDRNYRPIPGTQPTKYHTCYDEEFERVMQPDYDRLVDETPGGRFALLVD
                     EGGYAPTHNSFYSKKPTGDARVDLLHSRDKRIFNDPAGLKSASNTLPFLLQTYVRDTG
                     EVLSEISLPVFINNRHWGALRLGFDTTRMLTLSGHL"
BASE COUNT          845 a         1188 c         1209 g          770 t
ORIGIN      
        1 gaataccggc ctgtcacgcc gggggtcgcg ggttcgagtc ccgtccactc cgccagagaa
       61 tacaaaaggt gaacgcgtgc gcgttcacct tttttttatt gtcgcctcat tcgagggctg
      121 tgttccatgt tcgattttgt ccagaaaaac tccctgttca tcaaagttgt gctcggcggc
      181 gtggcgctga cctttgtcgg ttttggtgtg ggcagttata ccgccgctac cgatgacccc
      241 tatctggcca aggtggatgg cgtcaagatc tacaagcagg atatcgagcg cgcgctcaac
      301 ggccgcccgg ccgacgccgc tacccgtcaa ggcgtgctgg aaaacctggt gcgtaaagag
      361 ttgctgctgg cggacgcccg gtcgtctggc ctggtggtca gcgccgatga actgcggcgg
      421 gcgattctgg acatccccgg cctgcaagaa aacggcaagt tcagtaaaac tcaatatgag
      481 ctgttcttga agagccaggg cctgtcggcg ccgcactttg agaaaatgat tggcgacgat
      541 ctgctgattc aaaaacagct ggacagcatc gccggctctt cgctggtgtc caacgccggc
      601 attgagcgcg tccaccagct gattggccag tcgcgcgaga tcagcgcctg ggtgctgcgc
      661 ccgcagcagt ttgccactga ggtcaaaatt gacgacgagg cggtgaaagc ctactacaac
      721 gcgcaaagca aacgctatca gacaccagac atggtgcgcg cccagtatgt ggtgctcagc
      781 caggacaagc tggccgccag catcaaggtc agcgacgaag aaatccgcca gtatttcgaa
      841 cagcaccgtg ccgaattggc gccggagcag cgccgggtgg cgcatatcct gattgccgcc
      901 ggcaaggatg ccgacgccgc tgcgcgcacc aaggcgcgcg agcaggccga agcgctgctg
      961 aaagagctga aaaccaatcc tggccgtttt gccgaactgg cgcgcgcgcg ctcgcaagac
     1021 cctggctcgg ccgcccaggg cggcgatctg ggctattttg ccaagggcat gatggtcaag
     1081 ccgtttgaag acaccgcgtt caaactggcc aagggcgaga tgagcggcgt ggtggaaacc
     1141 gactttggtt atcacatcct gcgcctggac gatatcaagc agccggactt tgccgccgtc
     1201 aaggagcaag tcaccgcccg cctgcaaaag caaaaagcca ccggcgcact gcgcgagcag
     1261 gcggaaaaac tcaatgagct ggcctatcag caagccgaca gcctgaaagc cgccagcgac
     1321 acgctcaagc tgccgctgga aaccagcgac tggctggcgc gtgacagcaa gccgaccaac
     1381 ccggtgctgg cccaccccaa gctgctggat gccttgttca gcgatgatgt gttgaagggc
     1441 aagcacaata gcgaagccat cgaagtggcg ccgggcacgc tggtggtggt gcgcgtggcc
     1501 gagcacaagc cggcagcaca aaaaccgctg gaagaagtcg ccgccagcat tcgccagacg
     1561 ctgaccgaca gcaaggccgc cgaactggcc agcgagaagg gcaaaaccct gttggccacg
     1621 ctgaaagccg gtaaagcggc tgctggcgaa agctgggggc cgatgcaaac ggtgtcacgt
     1681 caggcgccgg tcagcctgtc gcgtgacgct gcacgcgtgg tgttctctgc cccggtggcc
     1741 aaactgccgg cctatgccgg cgccagcacg gataccggtg attatgtgat ctaccgcatc
     1801 agcaaggtca ccccggcgcc agccgccaag ccggaagacc aggcgcgcct gcgccagatg
     1861 ctgagtcagg caatgtccaa cgctgaagcc atggcctatg tagaaagcct gcgccagcgc
     1921 ttcaaggtgg tgcagcgccc gatcactggc ggtgaagaca gccagcagta agcctgtcgc
     1981 cctggccatg aaaaagccca tcgcccgcct ggtgtcagcc acggcgggcg atgggctttt
     2041 tacggcggcc tgaaccgccc tggcaaggtt tacaggtggc cgctgagggt gagcatgcgg
     2101 gtggtgtcaa aacccagccg tagcgcgccc cagtggcgat tattgatgaa taccggcagc
     2161 gagatttccg acagcacctc gccagtgtcg cgcacatagg tttgcagcaa gaacggcaag
     2221 gtgttgctgg cggatttcag cccggccgga tcgttgaaaa tgcgcttgtc gcggctgtgc
     2281 agcaggtcta cgcgcgcgtc gccagtgggt tttttcgagt agaacgaatt atgcgtgggc
     2341 gcatagccgc cttcatccac caacagggca aagcgcccac cgggggtttc gtccaccagg
     2401 cggtcgtaat ccggctgcat cacccgttcg aactcttcgt cgtagcaggt gtggtatttg
     2461 gttggctggg tgccaggaat cggccggtaa ttgcggtcaa acaggttgac tccacggtca
     2521 gccatctctt gcaggcgttt ttccagcatc tcccgatagc tgcgcgcgcg attgatggcg
     2581 tgatcaaaat ccccctcgcc aacgctgaaa cgcgacacca gctcttgcac ctgctcggaa
     2641 gcatgcgaca gctcctggga gaccttgtgg ctttgttgca gcttgtcggt gacttgctgg
     2701 ctcagttcgt cgatgtccga cacatggcgg ttcacctgct tgttggtggc ggcaaactgc
     2761 tccatggtgc gggcgatatc ggtgaggctg cgcgcggtgt tgtcgtaatc caccaccatt
     2821 ttttcaaaat gctgggtggc tttgctgact gtttcgctgg ccagccgggt atcgctggtg
     2881 atttgcccgg tttcgtccag ggtattgccc accaggcgca gcatgccgtc gatattctcc
     2941 gaaatctcgt cggtggccac tttcacccgc tcggccaatt tacgcacttc atccgccacc
     3001 acggcaaagc cgcgcccggc ctcgcccgcg cgggcggcct caatcgccgc attgagcgcc
     3061 agcagattgg tttggtcgga aatatccttg atcaggtcaa caatctgctt gatgctggcc
     3121 gaacgcagat tcaggccttc caccgtctgg ttaaagcggt tgaccttctg gctgactgcc
     3181 tcaatacgcg cagtgacatc cagcagctca gcgcaggaag atttggccac cgccagatta
     3241 tcctgggtgg tgtaagaaat atcctgggtt tgctcggaca cctgcgagat gccggccgtg
     3301 gtggcatcgc tggactggcg cacctcacgg gcaaattcgc cctgcttgcg cgcgctgccg
     3361 agtgaatcat tgatattgac cgccgaacgt gccgaatcca tggcaatgcg caccgtcatc
     3421 aaccgcacat tgctgatgat ctcgcgcatc ttgcgcaaaa agcggttgta ggattcggac
     3481 agctcgcgga tttcgtcaaa ggtcatcgcc gggatgtcgc gcgacagatc gccctcaccc
     3541 tggccgattt cattgaagat gctgatgatc atgcgcaagg ggcgcacgat cagaaaacgc
     3601 agataccaca ccatgaaggc gataaaggcc agcgagacaa accacagcgc caccaccgcc
     3661 tgtagggcgc ggttcatggt gttttgcagg gtttgcacaa cgtcgggttt aagttgggcg
     3721 gcttccagca catgctggac ttcgccaaaa taaaaccaca gcaaagcaac aaagcctaac
     3781 tggaaaagac tgataaagaa aaaactgcac agcttgcggc tgagcgagtt ccagaaggtt
     3841 ttctcgacca atgtataggt ttgccagaac caattcatta ttttcctccg tcctggtcat
     3901 gcagggttgg ccacggcatg cgatggccac tgacttcacc atgcgggcca cacgggctcg
     3961 ccaaagaagc gtgctgcatt gccgttgcgc caccggacac gagcccggcg ca
//
LOCUS       DAOPYX010000273         3998 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_29612_length_3998_cov_7.199341, whole genome shotgun sequence.
ACCESSION   DAOPYX010000273 DAOPYX010000000
VERSION     DAOPYX010000273.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 3998)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3998)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3998
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29612_length_3998_cov_7.199341"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..587)
                     /locus_tag="PKN55_11925"
     CDS             complement(<1..587)
                     /locus_tag="PKN55_11925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX71998.1"
                     /translation="MTTSISSSLATTFTATSTSSAPVPTSSSQATSKSRGHALMLEHL
                     WRIKDPSAPEPKILYTVTTDMLSGNSFDFLTYSDRRLLEKVYSYAEENNIDLEQVDLL
                     AFHMAGYRMAGPGGLLASKPGNAYGVEGKWVINAFDERDTKLAYQIKNGKAILSTEFD
                     RGYLDQALDPEKMPTHGFDFAFLAKMVKQFSDQAG"
     gene            complement(664..1122)
                     /locus_tag="PKN55_11930"
     CDS             complement(664..1122)
                     /locus_tag="PKN55_11930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX71999.1"
                     /translation="MTEYQPPAKGTVMKKWILICAMLGAAAQTHAVVGSWSKQTAHYP
                     IYFSGGYAEMPFNAPTSMPTLTSFITSVTYQWTGYGNGNTSRTVQICYSKKYSTVISN
                     CWDISNRQQNTIHNFDGFDASGKFWIRHQIWGGTYPAVSSALDTVTVMHQ"
     gene            complement(1632..2762)
                     /locus_tag="PKN55_11935"
     CDS             complement(1632..2762)
                     /locus_tag="PKN55_11935"
                     /inference="COORDINATES: protein motif:HMM:NF013087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HNX72000.1"
                     /translation="MMPLQKNRAAVRRFAGMMKFAGWLQRLPDRVTPPPFRLMQIGSA
                     FWQSRALYVAARLDVATRLGDRHLTADEIAALVLAQPDALYRLLRMLVAIGVFEEVSP
                     RVFANNRLSAPLRDDHPDGVRAIILLHHSDEMSRPWGEQLERCVREGGIPFQRTHGAA
                     LFDYMDQHPAFDALFAQAMDHVEALSGDSFVRDFDWSRFRRVIDMGGSRGSKAIAILK
                     RHPHLHALVVDRAQVIQGAAGHWQGDVAPSVLARLRFQAGNLLESAPMAINDQDIYLL
                     SAVFHGFDDPTCIQALRNLARACGGTGARIALLEMVMAESRADLACAAFDMQMFMGTR
                     GRERTLGEWQRVFDEGGCVLEEQVNLQSFVRMLVLQVKPEAG"
     gene            complement(2759..3451)
                     /locus_tag="PKN55_11940"
     CDS             complement(2759..3451)
                     /locus_tag="PKN55_11940"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HNX72001.1"
                     /translation="MVIGIRNADVSLFFIIHRRESPMNEHAGDSVRQRLLKAALDGFL
                     ADEYHKVTTRQIAERAGANIAMIRYYFGNKEGLYEEMIRETLGPLLDVLDGPMLSSVS
                     GFADLLHLYYDTMLARPEFPKLILKVLALNQGPGRRFIQQLLERGRTGAARKVVDLKT
                     HGQIAPTVDPDMLRMAFVSLAMTPMLLKDIFEKQMGQAMDAAFLTRLAEFNGRLFAAG
                     LQPSADQPQEAA"
BASE COUNT          887 a         1177 c         1053 g          881 t
ORIGIN      
        1 tgcccagctt gatcagaaaa ctgtttgacc atctttgcca aaaaagcaaa atcaaagccg
       61 tgtgtaggca ttttttcagg atccaatgct tgatcgagat agcctctgtc aaattcagtg
      121 ctcaatatgg ctttcccatt tttgatttga taagcgagct tggtatctcg ctcatcaaat
      181 gcattaatca cccactttcc ttccacgcca taggcgttcc caggtttact ggcaagaagt
      241 ccgcctggac cagccatgcg gtaacctgcc atatgaaaag ccagcaagtc cacttgttcc
      301 aagtcaatat tattttcttc tgcgtaggag tagactttct ccagtagtcg ccggtcagag
      361 tacgtcaaaa aatcaaacga attgccagac aacatgtctg ttgtgacggt atacaagatt
      421 tttggctcag gcgcggaagg gtctttgatc cgccacaaat gctccagcat cagggcatgc
      481 cctctggatt tactggtggc ctggctgctg gatgtgggta ctggggcaga agaagttgag
      541 gtggcggtga aagtcgtcgc caaactgctg cttatgctgg tggtcatgat actctcctta
      601 aatatacact ctgtgttttt gcgacagggt gttaaaatac ttccctatcg caagatcaaa
      661 ttgtcactga tgcatgacgg tcacagtgtc tagcgcactg gaaacggcag gataagtccc
      721 tccccaaatt tggtgtcgga tccagaattt ccctgaagca tcaaatccat caaagttatg
      781 gatagtattt tgctgtctgt tagaaatatc ccaacaattg ctaattactg ttgagtactt
      841 ttttgaatag cagatttgca ctgttctaga tgtattgcca ttaccatacc ccgtccactg
      901 atatgtaacc gacgtgataa acgaagtcag tgtcggcatc gaagttggag cattgaacgg
      961 catctcagca taccctccac tgaagtaaat ggggtaatgt gcagtctgtt tgctccacga
     1021 gcccaccaca gcatgggttt gcgcagcagc gccgagcatg gcgcatatca atatccactt
     1081 tttcatgacg gttcctttag caggtggttg atattctgtc attcaggcat cggtaatgcc
     1141 tatgaacaat ctaagcctac ctggcgaact cagtcaaatt tagcatagtt gaatgttgtt
     1201 tttttgcaaa atttttcttg aaaattaagt aattatacgt aatttgcgca atatttttca
     1261 caaaaaacac ccaaaaaaca tgcacaagat gcaggctctt accttgcttc tggatagcca
     1321 acatacatca tggcgacacg cgcccgcacg gtggctgtat ttgttcagcg cggtggcacc
     1381 accagcgttt cgccctctgc caacccagca cgaatttcca cccggccatc ctgcgtctcg
     1441 ccgacctgca cgctgcgcgg gcgcaacagc catctccgcc gccgcgtgat gtggtggaga
     1501 aggtcaagga acagttgttc tgagatccgg caacggaaag gggcgcggcg gcaggcatga
     1561 cgtcgcgccc cttcggcatg tgctgtttgc atgctgaagg gcccatgcct ggcccgtgct
     1621 tgtgtgccgg ttcaaccggc ttcaggtttc acttgcagca cgagcatcct gacgaaggac
     1681 tgaaggttca cctgttcctc caggacacat ccgccctcgt caaacacccg ctgccattcg
     1741 ccaagcgttc tttccctgcc gcgcgtgccc atgaacatct gcatgtcgaa tgccgcgcag
     1801 gcaagatccg cgcgtgattc ggccatgacc atttccagca gggcaatacg cgcacccgtg
     1861 ccgccacatg cccgggcaag attgcgcaga gcctgaatgc aggtcgggtc atcaaaccca
     1921 tgaaagacgg cgctcaacag ataaatgtcc tggtcgttga tcgccatcgg cgccgactca
     1981 agcagattgc cggcctgaaa acgcagccgg gcaagcaccg atggcgccac atctccctgc
     2041 caatgcccgg cggcgccctg aatgacctgc gcgcgatcca ccaccaaagc gtgcagatgc
     2101 ggatgccgtt tcaggatggc aatcgccttg ctgccccttg agccgcccat gtcgatcaca
     2161 cgccgaaagc ggctccagtc aaaatcccgg acaaaactgt ctccgctcaa tgcttccaca
     2221 tggtccatgg cctgggcaaa cagggcgtca aaggccgggt gctggtccat atagtcgaac
     2281 agcgctgcgc catgggtgcg ctggaacgga atcccgcctt cacgcacgca ccgttccagt
     2341 tgctcgcccc atggccggct catctcgtcg gaatggtgca gcaggatgat ggcccgcaca
     2401 ccatccgggt gatcgtcacg cagcggggcg gacagacgat tattggcaaa cacgcggggc
     2461 gagacctcct caaacacccc gattgccacc agcatccgca gcagccggta cagcgcgtcg
     2521 ggttgcgcca agaccagggc agcaatctca tccgcagtca gatgccggtc tcccaggcgg
     2581 gtggcgacat ctagccttgc cgcaacgtac aaggcccgcg attgccagaa ggccgacccg
     2641 atctgcatca gccggaatgg cggaggcgtc accctgtcgg gaaggcgttg caaccagccg
     2701 gcaaatttca tcatgccggc aaaccggcgg acagcggccc gattcttctg caagggcatc
     2761 atgcggcctc ctgtggctga tcggcggacg gctggaggcc ggcggcaaac aggcggccat
     2821 tgaattcggc caggcgggta aggaacgcag catccatcgc ctgccccatc tgtttttcga
     2881 aaatgtcctt caacagcatg ggcgtcatgg caaggctgac aaacgccatt cggagcatgt
     2941 ccgggtccac cgttggcgca atctggccat gggttttcag atccaccacc ttgcgcgccg
     3001 cccccgtgcg cccacgctcg agcaattgct ggataaagcg ccgcccgggc ccctgattca
     3061 gcgccagcac cttcaggatc agcttgggaa actccggccg ggccaacatc gtgtcgtaat
     3121 agagatgcag caaatcggca aaccccgaca cagatgacag cattggccca tccagcacat
     3181 ccagtaacgg acccaaggtc tcccggatca tctcttcata cagcccttcc ttgttgccga
     3241 agtaataccg gatcatggca atattggcgc cagccctttc ggcaatctgg cgtgtcgtga
     3301 ccttgtggta ttcgtcggca agaaagccat cgagcgccgc cttgagcagg cgttgccgga
     3361 cagaatcgcc agcgtgttca ttcatcggac tttccctgcg gtggatgatg aagaacaggc
     3421 taacatcggc attccggatg ccgataacca tttcgccaat caaatcaccc gctttgatgc
     3481 atcacgcctg gggaggacgg cgcgcggatg tgacggcgca ctggtggacg catggctgcc
     3541 cgtggtggca cgtgtccgtc ccggtcgtgc ctgcagccac gccgggtgat ccatccggca
     3601 aaaaaaaggg cgcccggtgc accgccagcg ccgggctccc cgcccaaagt cgtatgttgc
     3661 aatgcaaccg gatgatctat agtgacattg cctcagactg tgtgaggtct tgtgtgtctc
     3721 tcctcttctt gacgatcttg acgcccgcgc tgcgggcgtt tttttgtctc ctgccactaa
     3781 agtcgcattc tcccggcagc aacgcctccc tatagtggcc tggcccaccc gcacatggca
     3841 tgaccgtgac gggccgggtg cgccgccatc cggctggcgt tttcctgttc accttctgga
     3901 ggactgcatc atgcgtcaat ggcacacccc gcaagtggtc gagatcgccc tcggcctgga
     3961 aatcaactgc tacatgtgcg ctgagctgta aggcgcga
//
LOCUS       DAOPYX010000274         3937 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_30294_length_3937_cov_10.742916, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000274 DAOPYX010000000
VERSION     DAOPYX010000274.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 3937)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3937)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3937
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30294_length_3937_cov_10.742916"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..642
                     /gene="pyrF"
                     /locus_tag="PKN55_11945"
     CDS             <1..642
                     /gene="pyrF"
                     /locus_tag="PKN55_11945"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012696358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HNX72002.1"
                     /translation="SRLEPGSCRLKVGKELFTATGPHLVQTLVARGFEVFLDLKFHDI
                     PNTVASACKVAADLGVWMVDVHASGGRRMIEAAREALDTQSARPLLIAVTVLTSMDAS
                     DLAEIGLSDPAGQVSRLAALAQSSGADGVVCSAQEAPLLRGERGPAFKLVTPGIRLAD
                     SAADDQRRVMTPSAAIAAGSDYLVIGRPITRADEPFAVLQRINAEVAQARGAQ"
     gene            639..1961
                     /locus_tag="PKN55_11950"
     CDS             639..1961
                     /locus_tag="PKN55_11950"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014088137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HNX72003.1"
                     /translation="MKVSVIGSGYVGLVTGACLAEVGNQVLCLDVDPAKIALLQGGGV
                     PIYEPGLEDMIRRNAAAGRLSFTTDVAAAARFGEIQFIAVGTPPDEDGSADLQYVLAA
                     ARGIARHIERPTVVVDKSTVPVGTADRVRDAMAATLAERGADIAFSVVSNPEFLKEGA
                     AIDDFMRPDRIVVGVDDAHAAELMRRLYLPFQRNHERLIVMDVRSAELTKYAANAMLA
                     TRISFMNELANLAEALGADIEKVRQGIGSDPRIGYHFLYPGCGYGGSCFPKDVKALAR
                     TADEHGLPLRVISAVEAANDAQKRRLADKIIARFGADLTGRCFALWGLAFKPNTDDMR
                     EAPSLTLIDALLARGATVAAYDPVARDEAARVLAGRPGIRFADSMQSALDGADALAIV
                     TEWKEFRSPDFADLKARLRTPAIFDGRNMYDPASVREAGLEYHAIGRP"
     gene            1971..2933
                     /gene="rfaE1"
                     /locus_tag="PKN55_11955"
     CDS             1971..2933
                     /gene="rfaE1"
                     /locus_tag="PKN55_11955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase"
                     /protein_id="HNX72004.1"
                     /translation="MSLLETLSLNCADLSARLAGARVLVVGDVMLDRYWFGEVNRISP
                     EAPVPVAKITRIEERAGGAANVARNIAALGGQASLLSVVGDDEAASALETLLLRDGIA
                     TSLRRDPSISTTTKLRVVARQQQLIRIDFEEAPSHEILADKLDDFAARLAQHDVVILS
                     DYGKGGLTHVARMVELARAAGKPVLIDPKGDDWSKYAGATLLTPNKSEFRQVAGSWRD
                     EADLAQRAQTLRQQLQLDALLVTRSEEGMSLFDAHGAEHQPTLAREVFDVSGAGDTVI
                     ATLGLALAAGLSRPHAMHLANAAAGIVVAKLGTAVVDQQELFAA"
     gene            2963..>3937
                     /gene="rfaD"
                     /locus_tag="PKN55_11960"
     CDS             2963..>3937
                     /gene="rfaD"
                     /locus_tag="PKN55_11960"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007342468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HNX72005.1"
                     /translation="MTLVVTGAAGFIGSNLVKALNLRGDTDIIAVDDLTDGPKYRNLV
                     DCDIADYLDKDDFLARLKAGDYTGKLRAILHQGACSDTMEHNGKYMMANNYQYTLDLF
                     DWCQDHGVPLLYASSAAVYGAGRVFSESREHEAPLNVYGYSKFLFDQVVRRRFDQRRA
                     QVVGFRYFNVYGPREQHKGRMASVAFHHFNQYLQTGKVKLFEGYDGYPNGGQMRDFVS
                     VEDVVKVNLHFLDHPESSGIFNLGSGRAQPFNDVAVAAVNACRQHQGQAALSLAELVE
                     QGVLEYIDFPDALKGKYQSFTEANISALRAAGYAQPMLSVEEGVARYAQ"
BASE COUNT          703 a         1397 c         1224 g          613 t
ORIGIN      
        1 tcccggctgg agccgggcag ctgccggctg aaagtgggca aggaattgtt caccgccacc
       61 ggcccgcatc tggtgcaaac cttggtggcg cgcggttttg aggtgtttct cgacctgaaa
      121 ttccacgaca tccccaatac cgtcgcctcc gcctgcaagg tggccgccga cctgggcgtg
      181 tggatggtgg acgtgcacgc cagcggcggc cggcgcatga tcgaagccgc gcgcgaggcg
      241 ctggacaccc agtccgcccg cccgctgctg attgccgtca ccgtgctgac cagcatggat
      301 gccagcgatc tggccgaaat cggcctgtcc gacccggccg ggcaagtgtc gcggctggcg
      361 gcgctggcgc aatccagcgg cgccgatggc gtggtgtgct cggcgcaaga agcgccgctg
      421 ttgcgcggcg agcgcggccc ggcgttcaag ctggtcaccc ccggcatccg cctggccgac
      481 tccgccgccg acgaccagcg ccgggtgatg acccccagcg ccgccatcgc cgccggctcg
      541 gactatctgg tgattggccg gccgatcacc cgcgccgacg agccgtttgc cgtgctgcaa
      601 cgcattaacg ccgaagtggc gcaagccaga ggcgcgcaat gaaagtctcg gtgattggca
      661 gcggctatgt gggcctggtc actggcgcgt gtctggccga agtcggcaat caggtgctgt
      721 gcctggatgt cgatccagcc aaaatcgccc tgttgcaggg cggcggcgtg ccgatttacg
      781 agcccggcct ggaagacatg atccgccgca acgccgccgc cggccggctg agcttcacca
      841 ctgacgtggc cgccgccgcc cgctttggcg agattcagtt tatcgctgtg ggcaccccgc
      901 ccgacgaaga cggctcggct gatctgcaat atgtgctggc cgccgcgcgc ggcattgccc
      961 gccatatcga gcgccccacc gtggtggtgg acaaatccac cgtgccggtg ggcaccgccg
     1021 accgcgtgcg cgacgccatg gccgccaccc tggccgaacg cggcgccgac atcgccttca
     1081 gcgtggtgtc caacccggag tttctcaaag aaggcgcggc gattgacgac tttatgcgcc
     1141 cggaccgcat cgtggtgggg gtggacgacg cccatgccgc cgaactgatg cgccggctgt
     1201 acctgccgtt ccagcgcaac cacgagcggc tgatcgtcat ggacgtgcgc tcggccgagc
     1261 tgaccaaata cgccgccaac gccatgctgg ccacccgcat ctcgttcatg aacgagctgg
     1321 ccaacctggc cgaagcgctg ggcgcggata ttgaaaaagt ccgccagggc atcggctctg
     1381 acccgcgcat tggctaccat tttctctacc ccggctgcgg ctatggcggc tcgtgctttc
     1441 ccaaggacgt caaagccctg gcgcgcaccg ccgacgaaca cggcctgccg ctgcgggtga
     1501 tcagcgccgt cgaagccgcc aacgacgcgc aaaagcgccg gctggccgac aaaatcatcg
     1561 cccgctttgg cgctgacctc accggccgct gctttgccct gtggggcctg gccttcaagc
     1621 ccaacaccga cgatatgcgc gaagcgccca gcctgacgct gatcgacgcc ctgctggcgc
     1681 gcggcgccac ggtggccgcc tacgacccgg tggcgcgcga cgaagccgcc cgcgtgctgg
     1741 ctggccgccc cggcatccgc tttgccgaca gcatgcagag cgcgctggac ggcgccgacg
     1801 cgctggcgat tgtcaccgaa tggaaagaat ttcgcagccc ggactttgcc gacctcaaag
     1861 cccgcctgcg caccccggcc atttttgacg gccgcaatat gtacgacccg gccagcgtgc
     1921 gcgaagccgg gctggaatac cacgcgattg gccgcccctg aaagtattgc atgagcctgc
     1981 ttgaaaccct gtccctgaac tgtgccgacc tttccgcccg cctggccggc gcccgcgtgc
     2041 tggtggtcgg cgacgtgatg ctagaccgct actggtttgg cgaagtcaac cgcatctcgc
     2101 ccgaagcccc ggtgccggtg gccaaaatca cccgcatcga agaacgcgcc ggcggcgcgg
     2161 ccaatgtggc gcgcaatatc gccgccctgg gcggccaggc cagcctgctg tccgtggtgg
     2221 gcgacgacga agccgccagc gcgctggaaa ccctgctgct gcgcgacggc atcgccacct
     2281 cgctgcgccg cgaccccagc atcagcacca ccaccaagct gcgcgtggtg gcccgccagc
     2341 aacaactgat ccgcatcgac tttgaagaag cccccagcca cgaaatcctg gccgacaagc
     2401 tggacgactt tgccgcccgc ctggcgcagc acgacgtggt gattttgtcc gactacggca
     2461 agggcggcct cacccacgtc gcccgcatgg tcgagctggc gcgcgccgcc ggcaagccgg
     2521 tgctgatcga ccccaagggc gacgactgga gcaaatacgc cggcgccacc ctgctcaccc
     2581 ccaataaaag cgaattccgc caagtggccg gcagctggcg cgacgaagcc gacctggccc
     2641 aacgcgccca gacgctgcgc cagcaactgc aactcgacgc cctgctggtc acccgcagcg
     2701 aagaaggcat gagcctgttc gacgcccacg gcgccgaaca ccagcccacc ctggcgcgcg
     2761 aagtgtttga cgtgtcgggc gccggcgaca cggtgattgc caccctgggc ctggcgctgg
     2821 ccgccggcct gagccgcccg cacgccatgc acctggccaa cgccgccgcc ggcatcgtgg
     2881 tggccaagct gggcaccgcc gtggtggacc aacaggaact gtttgcggcc tgacgccgcc
     2941 cgaaactctg gagcttaccc ccatgaccct cgtcgtcacc ggcgctgccg gctttattgg
     3001 ttccaacctg gtcaaggcgc tcaacctgcg cggcgacacc gacatcatcg ccgtcgatga
     3061 cctcaccgac ggccccaaat accgcaacct ggtggactgc gacatcgccg actacctgga
     3121 caaagacgac tttctcgccc gcctgaaagc cggcgactac accggcaaac tgcgcgccat
     3181 cctgcaccag ggcgcctgct ccgacaccat ggagcacaac ggcaaataca tgatggccaa
     3241 caactatcag tacacgctgg acctgtttga ctggtgtcag gaccacggcg tgccgctgct
     3301 gtacgcctcc agcgccgccg tctacggcgc tggccgggtg ttcagcgaat cacgcgaaca
     3361 cgaagccccg ctgaacgtgt atggctactc gaaatttctg ttcgaccaag tggtgcgccg
     3421 ccgctttgac cagcgccgcg cgcaagtggt gggctttcgc tacttcaacg tctacggccc
     3481 gcgcgaacaa cacaaaggcc gcatggcctc ggtggccttt catcacttca accaatacct
     3541 gcaaaccggc aaggtgaaac tgtttgaagg ctacgacggc taccccaacg gcggccagat
     3601 gcgcgacttt gtttcggtgg aagatgtggt gaaggtcaac ttgcactttc tggaccaccc
     3661 ggaaagctcc ggcatcttca atctgggcag cggccgcgcc caaccgttta acgacgtcgc
     3721 cgtcgccgcc gtcaacgcct gccgccaaca ccaaggccaa gccgcgctca gcctggccga
     3781 actggtggaa caaggcgtgc tggaatacat cgacttcccg gacgcgctga aaggtaaata
     3841 ccaaagcttt accgaagcca atatcagcgc cctgcgcgca gcgggctatg cacagccgat
     3901 gctgagcgtg gaggagggcg tggcgcggta tgcgcag
//
LOCUS       DAOPYX010000275         3907 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_30645_length_3907_cov_7.357996, whole genome shotgun sequence.
ACCESSION   DAOPYX010000275 DAOPYX010000000
VERSION     DAOPYX010000275.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 3907)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3907)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3907
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30645_length_3907_cov_7.357996"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..868)
                     /locus_tag="PKN55_11965"
     CDS             complement(<1..868)
                     /locus_tag="PKN55_11965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003791505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HNX72006.1"
                     /translation="METQSTYNYKVVRQFAVMTVIWGIVGMLVGVIIAAQLVWPDLNF
                     GPWLHFGRLRPLHTNAVIFAFGGCALFATSYYVVQRTCQVRLFAEKLAAFTFWGWQLV
                     IVLAAVTLPLGFTSGKEYAELEWPIDLLITVIWVAYAIVFFGTIAVRKVKHIYVANWF
                     YGAFILTVALLHIVNSLEIPVSLTKSYSVYAGAVDAMVQWWYGHNAVGFFLTAAFLGM
                     MYYFVPKQAGRPVFSYRLSVVHFWALIFTYMWAGPHHLHYTALPDWTQSLGMVFSLIL
                     LAPSWGGMINGIM"
     gene            1345..3096
                     /locus_tag="PKN55_11970"
     CDS             1345..3096
                     /locus_tag="PKN55_11970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014086304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HNX72007.1"
                     /translation="MIHPLHYRLCVADPAAHYFDVTLSIQALTPGPLTLRLPCWIPGS
                     YLLREFARHIVSLHAQADGAAIAVTKTDKHSWTLDPGQARRLEVHYRVYAFDLSVRGA
                     YLDLERGFFNGSSVFLEVAGRADEPVSVEIAAPEHPTCADWKLVTSLPRLGRAARHGF
                     GRFCADNYAELIDHPVEMAQIDLVRFTAGGAPHEIAVSGQHRGDLKRLARDVKAVCEW
                     QIRLFGAPPPFTRYVFLLYVGQDIYGGLEHRASTALMADRAALPAVGEDAPSKAYQDL
                     LGLFSHEYFHAWNVKRIAPAVFTPHDLYQENYTRLLWAFEGVTSYYDDLALARSGVIS
                     AEQYLKRLSDTLTAVERTPGQYVQNLEDASFDAWIKYYRQDENSPNSLVSYYTKGALV
                     ALCLDLLIRRDTRGARSLDDVMRALWQRWLADGQGVAEAEWEAIASQISGLDLSDFFD
                     RALRSVEPLPLAELLASVGVTLSHSSGDSQAPTLGVKTERDPLGWKIKCAYQDGPAQQ
                     AGLSGGDVLIALDGLRIHDLDAMLARYQAGEQLSVHAFRRERLLHVSVTLQARPHDHC
                     ALAFEPERVKGWLSTQG"
     gene            complement(3175..3648)
                     /locus_tag="PKN55_11975"
     CDS             complement(3175..3648)
                     /locus_tag="PKN55_11975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX72008.1"
                     /translation="MSEVSSAEAVAPAPIFAPRAFTAQIPSVLAVLTLLACFAMFYYF
                     IDLSSKSGAQLVEMKAAALEVSQLTQQMRQLPPEKTAQYAKAQDDLVLAQKVLDNARS
                     VQDAVKEQQSATKDFILYILGVLSSAVTTILGYYFGSSSSSAQKTEVLKAMTQKE"
BASE COUNT          882 a         1181 c         1109 g          735 t
ORIGIN      
        1 tcatgatgcc gttgatcatg ccaccccagg acggagccag cagaatcagc gagaacacca
       61 tacccagcga ctgagtccag tccggcaggg cggtgtagtg cagatggtga ggacctgccc
      121 acatataggt gaagatcagc gcccagaagt gcaccacgga caggcgatag gagaacaccg
      181 gacggccagc ttgcttgggc acgaagtagt acatcatgcc caggaaggcg gcggtcagga
      241 agaagcccac agcgttgtga ccgtaccacc attgcaccat ggcatccaca gcgccagcgt
      301 acaccgagta ggacttggtc agggaaaccg ggatttccag gctgttgacg atgtgcaaca
      361 gggccacagt caggatgaag gcaccgtaga accagttggc cacatagatg tgcttgactt
      421 tgcgcacggc gatggtgcca aagaacacaa tcgcgtaagc cacccagatc acagtgatca
      481 agagatcaat cggccattcc agctcggcgt attccttgcc ggaggtgaaa cccagcggca
      541 gggtgacggc ggccagcaca atgaccagtt gccagcccca gaaggtgaag gcggccagtt
      601 tctcggcgaa caggcgaacc tgacaggtgc gctgaacgac gtagtaggag gtggcgaaca
      661 atgcgcaacc gccaaaggcg aaaatcacgg cattggtatg cagcggacgc agacgaccaa
      721 agtgcagcca cggaccaaag ttcaggtccg gccacaccaa ctgcgcggcg ataatcacgc
      781 ccaccagcat gccgacgatg ccccagatca cggtcatcac ggcaaactgg cgcaccacct
      841 tatagttata agtggattgc gtttccattt agggtttctc caaggttgct aacacaagaa
      901 tcaaaaaccg tcgagcaggc ctgttggaaa cgccccatat gcccccatat ggttgtgatg
      961 cgcttccacc ggctcgggcc ggggactggc gccgcagggc caaaagccct tgtgtgcgac
     1021 gcggtcagct acacgccccc gtatctgaat cgacccattt tacatcgaag gggccgcaca
     1081 ggccacacag atgcatggcc aagtcagtca acccttgcct gacgcaagac tcaattccag
     1141 ccaactcatt atattgtcat gccttgacaa ggtgttgacg taggtcaacc ccaagccagc
     1201 ccatcagccg atttccccgt atttttacca tgccagggga cttgacctga ctgagtcacg
     1261 ccatcagcat gggcaaaagg catacacatc ttcgcctgca tgcggcacac tgtcagtccg
     1321 actttatgtg cacacgagcc cgccatgatc caccccttgc attaccgcct gtgcgtggcc
     1381 gacccggccg cacactattt tgacgtcacc ctgtccatcc aagcgctgac ccctggccca
     1441 ctcaccctgc gtctgccctg ctggattccc ggcagctatc tgctgcgcga gtttgcccgt
     1501 cacatcgtca gtctgcacgc ccaggccgac ggcgcggcca ttgccgtgac caaaaccgac
     1561 aaacacagct ggacgctcga ccccggccag gcccgccggc tggaggtgca ttaccgggtg
     1621 tacgcgtttg atctctcggt gcgcggcgcc tatctcgacc tggagcgcgg cttcttcaat
     1681 ggcagcagcg tgtttctgga agtcgccggc cgcgccgacg aaccagtcag cgtggaaatt
     1741 gccgcaccgg aacacccgac ctgcgccgac tggaagctag tcaccagcct gccgcgcctt
     1801 gggcgcgccg cccggcatgg ctttggccgc ttctgcgccg acaactatgc cgagctgatc
     1861 gaccacccag tggaaatggc ccagatcgac ctggtgcgct tcaccgcagg cggcgcgccg
     1921 cacgaaattg ccgtcagcgg ccagcatcgc ggtgacctca agcgcctggc gcgcgacgtc
     1981 aaagccgtat gcgaatggca aatccgcctg tttggcgcac caccgccgtt tacccgctat
     2041 gtgtttttgc tgtatgtcgg ccaggatatt tatggcggac tggaacaccg cgcgtccacc
     2101 gccttgatgg ccgaccgcgc cgcactgccg gcagtgggag aagacgcgcc cagcaaggcg
     2161 tatcaggatt tgctcggttt gttcagccat gaatattttc acgcctggaa cgtcaagcgc
     2221 attgccccgg ccgtgtttac cccgcacgac ctgtatcagg aaaactacac ccggctgctg
     2281 tgggcgtttg aaggcgtcac ctcgtattac gacgacctgg cgctggcgcg cagcggggtg
     2341 atcagcgccg agcagtatct caagcgcctg tccgacacac tgactgccgt cgagcggacg
     2401 ccgggccaat atgtgcaaaa tctggaagac gccagtttcg acgcctggat caaatactac
     2461 cggcaagacg aaaacagccc caacagcctg gtgagctatt acaccaaggg cgcattggtg
     2521 gctttgtgcc tggatttgtt gattcgccgc gacacccgtg gtgcgcgttc gctggatgac
     2581 gtgatgcgcg cgctgtggca acgctggctg gcagatggcc agggcgtggc ggaagccgaa
     2641 tgggaggcga ttgccagcca aatcagcggg ttggatttat ccgacttttt tgaccgcgcc
     2701 ttgcgcagcg ttgagccctt gccattggcc gaactgctgg ccagcgttgg cgtcacgctc
     2761 agccacagca gcggcgatag ccaggcgccg acgttggggg tgaaaaccga gcgtgatccg
     2821 ctgggctgga agatcaaatg cgcctatcag gacggcccgg cgcagcaggc tggcctatcg
     2881 ggcggcgatg tgctgattgc actggatggc ctgcgcatcc acgacctgga cgccatgctg
     2941 gcgcgctatc aagcgggtga acagctcagc gtgcacgcct tccgccgtga gcggctgctg
     3001 cacgtcagcg tcaccctgca agcgcgcccg cacgaccatt gcgcgctggc gtttgagcca
     3061 gagcgggtga agggctggtt atcgacacag ggctgagatg gcactgatgg tgcgccagaa
     3121 ccgcagttct ggcgccgccc tcccgacaga aaaaacacgc caaatagatt tattctattc
     3181 tttttgcgtc atggctttca gcacttccgt tttttgcgca ctgctgctgg aactgccaaa
     3241 gtaatagccc agaatggtcg tcaccgcgga tgacaacacg ccaagaatat acaaaatgaa
     3301 atccttggtg gcgctttgct gttccttgac ggcatcctgc accgaacggg cgttgtccaa
     3361 cactttctgc gccagcacca gatcatcctg tgccttggcg tactgagcgg ttttctctgg
     3421 cggcaattgc cgcatttgtt gggtaagctg cgacacctcc agcgcagcgg ccttcatctc
     3481 gaccagctgt gcgccacttt ttgaagataa atcaataaag tagtaaaaca tggcaaaaca
     3541 ggccagcagg gtcagcacag ccagcacgga aggtatctgt gcggtgaacg cgcggggagc
     3601 aaaaatgggg gccggcgcaa ccgcctcagc ggaagaaact tcagacatga tcaaactcct
     3661 gaaaaatcca atatggcatt catcaccaaa gcctatgtgc tttggctgac gccagcataa
     3721 aacaaatcat ggatttccga ggcgctgctt tttgcaagct ggatattatc gccagcaaaa
     3781 aacccataca aataaaacac catcagtagc tttactctac cgatgaagaa aagcttagca
     3841 tctcttgcta gggcgtgtca tcaatcaata cccgaagggg ttgaaggaca caggtcaggc
     3901 accaatg
//
LOCUS       DAOPYX010000276         3906 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_30663_length_3906_cov_2.971176, whole genome shotgun sequence.
ACCESSION   DAOPYX010000276 DAOPYX010000000
VERSION     DAOPYX010000276.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 3906)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3906)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3906
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30663_length_3906_cov_2.971176"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..970
                     /locus_tag="PKN55_11980"
     CDS             <1..970
                     /locus_tag="PKN55_11980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX72009.1"
                     /translation="VTLHDVRCLRNARNGLRLEGGSGLTAMNCQFNFSGRGAFASAVS
                     AGVAVVPDSTSFVNQGVFMDCEMIDNVGPGFYSTGTNSSDLSIYSSTLIGTSSYAAQP
                     NSPRVHFTDCTLAGQVVTPFAETTTERGRATQFTRCRFTDALDYNGQVYTHSSWYLLD
                     FGNNSAGVKLTECQVDTVTAKLGYSNGLMIITDTRMTQANNTGTSNIQAVFVGNCAID
                     TAGSNPLTSSVVLGRLIINGAETIVYDPPQRFNRLLGKDSGGDKMQRIQHFHTPGASV
                     LASAPAVQGDVVYSTAPAVGGYAGWICVQGGDSTTSVWKGFGQIVN"
     gene            complement(1046..1300)
                     /locus_tag="PKN55_11985"
     CDS             complement(1046..1300)
                     /locus_tag="PKN55_11985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX72010.1"
                     /translation="MQAIASVHACVREADAAGTAQEIALMVDQQATTAQDIASGMETV
                     NAMSEQNPSGVRRITEDARQLRGAAQALHELVAHVAAYPA"
     gene            1777..3414
                     /locus_tag="PKN55_11990"
     CDS             1777..3414
                     /locus_tag="PKN55_11990"
                     /inference="COORDINATES: protein motif:HMM:NF024140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNX72011.1"
                     /translation="MKFTPLTLKQRVMLGIAALFALLLALAAAGWHGVRDNRATIERM
                     AQVDARKVSLGNDVASILSQMATELYLLVEEDQPERYEKFKQGLPEKLSIMSQRRSEL
                     LELVTESEERQILNELAGRRTEFVSSVEQVLKHLDTGEAPQARDQFQRRCLPALVLYS
                     QTMDRFQHIQHQKLNNNGKQASEKARQLETILAGLSVAAAVLAIGAGIWIVRSVTRPL
                     GGDPRVVSDAMSHIASGNLTIQASLLNAAPESLLGKLAAMRAGLSALIGNVHGASLEV
                     GAAARDLSASCEQISVGAIAQGQSTATMAAAVEELVGNIETLSSTSVRVQEVAERSEA
                     LAGNSDHLLSQAATEINRMIDTIDNSAQDVAQLARRTHEIGRIVGVINDIADQTNLLA
                     LNAAIEAARAGEQGRGFAVVADEVRKLAEHTTRATTEIDEMIRTIQQQTELAAENLLQ
                     GEQVVTFGVTLVRELVDPLRQLREGAGDTRRELGELISALEEQSQAARHIGSHVERVA
                     NAAEQFGAVARGSADTANMLLGVVTRLDGEVAHFRLA"
     gene            complement(3494..>3906)
                     /locus_tag="PKN55_11995"
     CDS             complement(3494..>3906)
                     /locus_tag="PKN55_11995"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNX72012.1"
                     /translation="RRCAAGGKQRRQGAVGGATTQIASMIDAVQRDTREVVGSMSAAA
                     PQVESSVGKAQSAARALEEIRAGAALALDNIRDVASATQEQSVANQRVAEHIERIANM
                     VAQSADTASQARHSAQQLEALADELNAAVAKFQV"
BASE COUNT          754 a         1103 c         1209 g          840 t
ORIGIN      
        1 ggtgacgctg cacgatgtgc gctgtctgcg caatgcgcgc aatggtctgc gtttggaggg
       61 cggcagtggc ttgaccgcca tgaattgcca gtttaatttc agtggccgtg gtgcgtttgc
      121 ttctgccgtg tcggccgggg tggccgtggt gccggacagt acatcctttg tcaatcaagg
      181 ggtgttcatg gactgcgaaa tgatcgacaa cgttggcccc ggtttttatt ccaccggcac
      241 caacagctcg gatctgtcga tttattccag cacgctcatc ggcacctcgt cctacgccgc
      301 ccagcccaat agcccgcgtg tgcatttcac ggattgcacc ctggcaggac aagtggtgac
      361 cccgtttgcc gaaaccacca ccgaacgtgg tcgtgccacc cagtttaccc gttgccgttt
      421 taccgatgcc ctggactaca acggccaggt gtacacccac tcaagctggt atctgctgga
      481 ctttggcaat aacagtgcgg gcgtcaagct gaccgagtgc caggtggaca ccgtcacggc
      541 caagctgggc tacagcaatg gcttgatgat catcaccgat acccgcatga cccaggccaa
      601 caacaccggg acatccaata tccaggctgt atttgtgggc aactgcgcca ttgatacggc
      661 gggcagcaac ccgctgacca gttcggtggt gctggggcga ctgatcatca atggtgccga
      721 aaccattgtg tacgatccgc cgcaacgctt taaccgcttg ctgggcaagg atagcggcgg
      781 tgacaagatg cagcgcatcc agcatttcca tacgccgggt gcttcggtgt tggccagcgc
      841 gccggccgtg caaggggatg tggtgtacag cacggccccc gctgtaggag gctatgctgg
      901 ctggatttgt gtgcaaggtg gcgatagcac caccagcgtc tggaaaggct ttggtcagat
      961 cgtcaactaa gcctgccatc ttcccccgca gggcgtatgc cctgcctttg ccagcccggc
     1021 gcgccttgtg cgcgccgggc tggcgtcatg ctggataggc ggccacatgc gccaccaact
     1081 catgcagcgc ttgcgcggca ccgcgcaact gacgtgcatc ctcggtaatg cggcggaccc
     1141 cgcttggatt ttgctcgctc atggcgttga cggtttccat gccgctggcg atgtcttggg
     1201 cggtggtggc ttgctgatcc accatcaggg cgatttcttg tgcggtgccg gcggcatccg
     1261 cctcgcgcac gcatgcatgg acgctggcga tggcctgcaa ggcatcgtca gtgcgttgac
     1321 ccgcaaatat cgccagtgct gtacgcggtg cgctcagtca atttgcgtac ctcgaagtgc
     1381 acaaatgatg ggcatctcag gagactagag gactgtgtgc gtgagctcag gctcccatcg
     1441 ttgagtcatc ccgttgtaac gtcatttctg catactatgg tgtgtcatga gcctaaattc
     1501 ctcaatttgc ttgatagcaa gcatctggtt accacgacaa tcaagaaagc cttttatgaa
     1561 tgcccaggct ccgttctgtg ctcagcgcat cagtctgcaa ttgactcgcc tctgcgaagc
     1621 gatggcgagc ttgcgcgctg aaatggcgcg tgtatgtcag caattgcccg caccctctgt
     1681 gcctcgtagt caatgaaaaa taaatgcatg ttttacttcg gccggctgca ttgtcggctt
     1741 tttttatcgc caacgaaccg ttaaggtaaa gacgacatga agttcactcc gctgacgctt
     1801 aagcaacggg tgatgcttgg cattgcggca ttatttgcgt tattgctggc gctggctgcg
     1861 gctggctggc atggcgtccg ggacaaccgg gcaaccattg agcgcatggc gcaagtggat
     1921 gcccgcaagg tcagcctggg gaacgatgtc gcttctatct tgagtcagat ggcgactgaa
     1981 ctttatctct tggtggaaga agatcagccc gagcgttatg aaaaattcaa acaaggcttg
     2041 cccgaaaaac tgtcgatcat gagtcagcgc cgcagtgaat tgctggagtt ggtgactgag
     2101 tcagaagagc ggcagatact caatgaactg gccggacgca ggactgagtt tgtcagcagt
     2161 gtcgagcagg tattgaagca tctggatacc ggagaagcgc cacaggcccg tgatcagttt
     2221 cagcgacgtt gcttgccggc gttggtcttg tattcacaaa ccatggatcg tttccagcat
     2281 attcagcacc agaagctgaa caataatggt aaacaggcca gcgaaaaagc ccgacaactg
     2341 gaaacgattc tggcggggct gtctgtggcg gcggccgttt tggcgattgg cgcaggcata
     2401 tggatcgtgc gcagtgtcac gcggccgcta ggcggggatc cccgtgtggt cagtgatgca
     2461 atgtcgcata ttgcttcggg caatttaacc atacaagcga gcttacttaa cgctgcgccg
     2521 gaaagcctgt tgggcaaact ggccgccatg cgtgccggct tgagtgcctt gatcggcaat
     2581 gtgcatggtg cttccttgga ggttggtgcg gcggcacgag acctttcagc ctcttgcgag
     2641 caaatttctg ttggtgcaat tgcacaaggc cagtccacag ccactatggc cgccgcggta
     2701 gaagaattgg tgggaaatat tgaaacgctt tccagcacct cggtgcgtgt gcaagaagtg
     2761 gctgagcgct cagaggcctt ggcggggaat agtgaccatc tactcagtca ggccgccact
     2821 gaaatcaacc ggatgatcga caccatcgac aattcggccc aggatgtggc gcaacttgct
     2881 cggcgtacac atgaaatcgg ccgtattgtt ggcgtaatta acgacatcgc cgatcaaacc
     2941 aatctcctgg cgctgaatgc ggcgattgaa gctgctcgcg cgggcgagca aggccggggc
     3001 tttgccgtgg tggcggatga agtgcgcaag ctggcggaac acacaacccg ggccacgacc
     3061 gaaattgacg agatgatccg cacgatccag caacaaaccg agctggcggc tgagaattta
     3121 ttgcaaggtg agcaggtggt gacatttggc gtgacgctgg tgcgcgaact ggtcgatccg
     3181 ctgcgccaac tgcgcgaagg cgcaggcgac acccgccggg agctgggcga gttgatttcc
     3241 gcgctggaag agcaaagcca ggctgcccgc catattggca gccatgttga acgggtggcc
     3301 aacgcggcgg agcaatttgg cgcggtggcg cgcggctctg ctgacaccgc caatatgctg
     3361 ctgggcgtgg tgacccggct ggatggcgag gtggcgcact ttcgcctggc gtgagtcggt
     3421 ggcggggatc aagcataaaa aacggcgcgc ccagtgggcg cgccgttttg catgacggac
     3481 aaaccggcct ggcttacacc tggaacttgg ccacggcggc attcagctcg tcagccagcg
     3541 cttccagctg ctgggcgctg tgccgtgcct ggctggcggt gtctgccgac tgcgccacca
     3601 tattggcgat gcgctcgata tgctcggcca ctcgttggtt ggccacgctt tgctcttggg
     3661 tggcgctggc cacgtcgcgg atattgtcca gcgccagcgc cgcgccggcg cggatttctt
     3721 ccagcgcgcg cgccgccgac tgcgccttgc ccacgctgct ttccacctgc ggcgcggcgg
     3781 cgctcatgct gcccaccacc tcgcgggtgt cgcgctgcac ggcgtcgatc atgctggcga
     3841 tttgtgtggt ggcgccgccg actgcgcctt gccgacgctg ctttccacct gcggcgcagc
     3901 ggcgcc
//
LOCUS       DAOPYX010000277         3905 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_30671_length_3905_cov_7.776104, whole genome shotgun sequence.
ACCESSION   DAOPYX010000277 DAOPYX010000000
VERSION     DAOPYX010000277.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 3905)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3905)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3905
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30671_length_3905_cov_7.776104"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..146
                     /locus_tag="PKN55_12000"
     CDS             <1..146
                     /locus_tag="PKN55_12000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012698318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase"
                     /protein_id="HNX72013.1"
                     /translation="VHALPLPGTRYDCGSKLGYLKASVDYALKHHEVGTAFAHWLRAH
                     PAR"
     gene            227..769
                     /locus_tag="PKN55_12005"
     CDS             227..769
                     /locus_tag="PKN55_12005"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017509678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine-guanine phosphoribosyltransferase"
                     /protein_id="HNX72014.1"
                     /translation="MPNVAEARGLLNSADVLFTADQVSAAVEKMAEDITALLGNSYPL
                     VLSVMGGAVVFTGQLLPRLAFPLDFDYVHVSRYGDKTRGGELVWRQAPKEDVRDRVVL
                     VLDDILDEGETMAAIRDKVLAMGAKSFHSAVFANKLISKAKPIAADFVGIDVPDRYVF
                     GYGMDVRGAWRNLPAIYALK"
     gene            965..1720
                     /locus_tag="PKN55_12010"
     CDS             965..1720
                     /locus_tag="PKN55_12010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018152904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family protein"
                     /protein_id="HNX72015.1"
                     /translation="MPARLARHLLRHHRTPAANLQLGGMLAWIAGAANASGFLVVHQY
                     TSHMTGMVSSLADALALGQLSLASSTLISIACFVLGSACCALCVQWARRRVRHEYALA
                     LLIQAVLMLLFGLLAGRVAPGVSLTLHMTAWSLCLMMGWQNAMITKLSHAEIRTTHVT
                     GLLTDIGIGLGRWLGPAQDRLTRRRARRKLAVQAAIVGLFFIGGLCGAWTFQWVGVVA
                     SVPLALLLIAMALAPLWDDWRAWHRWQRMHRVN"
     gene            1740..2495
                     /locus_tag="PKN55_12015"
     CDS             1740..2495
                     /locus_tag="PKN55_12015"
                     /EC_number="2.4.2.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018150489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioinosine phosphorylase"
                     /protein_id="HNX72016.1"
                     /translation="MIAIIGGSSLSQLPGLAITHRQVVRTPYGEPSCALTFGLLAGRT
                     VVFLARHGHGHMHAPHEINYRANLWALKSQQVRHVLAIGSVGGIHLGLAPGALAIPDQ
                     LLDYTWGRKHTFFEGPESPVVHVDFTQPYSATLRQCLLDGAQHAGVEALDGGTYACTQ
                     GPRLETAAEIARLRRDGADMVGMTGMPEAALARELDMEYACLAVVANYAAGCGDSIGK
                     VDFTAAGAVMGEAMDKVARVLEASLALGAHLGH"
     gene            2539..3087
                     /locus_tag="PKN55_12020"
     CDS             2539..3087
                     /locus_tag="PKN55_12020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018150340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HNX72017.1"
                     /translation="MSVRSILKMGDVRLLQQARPVEDFTAPWLATLLADLHDTMAEHH
                     GAGLAAPQIGEPWQVVVFGSGAPNPRYPDAEPVPATVLINPILTPLGAAMEEDWEGCL
                     SIPGLRGVVPRYRELRYQGFDAHGQPIDRTVSGFHARVVQHECDHLWGMLYPMRMKDM
                     SQLGFTDVLFPELALVAAAEPR"
     gene            complement(3235..>3905)
                     /locus_tag="PKN55_12025"
     CDS             complement(3235..>3905)
                     /locus_tag="PKN55_12025"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNX72018.1"
                     /translation="GAGANQMAQSCEETRAAARTVEEAQATIQELYQAVTHIGVVTDT
                     IREIADQTNLLALNAAIEAARAGEQGRGFAVVADEVRKLAERTGHSTSEINQLVARVR
                     AVTDSAVASMSQVGERTTQGEQHLSDTAHSLDEILHASAEVDHMMRSIASTNTQQSAA
                     ARELAERMQTISGRIEASTREIASAGDLIHDLSVKADEMGNLVRHFATDAHAANQAQL
                     QAAA"
BASE COUNT          605 a         1177 c         1300 g          823 t
ORIGIN      
        1 cggtgcatgc cctgccgctg cctggaacgc gctatgattg cggttccaaa ctgggctatc
       61 tgaaagccag tgtggattac gcgctgaaac accacgaggt gggcacggcc tttgcccact
      121 ggctgcgcgc gcatccggca cgctgattat ccccggtttt acatccgcgc cgcgccttcg
      181 gccaccccgg agcgcggcgt gtgtttttct tcaagagaga atactcatgc ccaatgttgc
      241 tgaagcacgc ggcctgctca atagcgccga tgtattgttt actgccgatc aggtgagcgc
      301 cgccgtggaa aaaatggccg aagacatcac cgccctgctg ggcaacagct acccgctggt
      361 gctgtcggtg atgggcggtg cggtggtgtt caccggccag ttgctgccgc gcctggcgtt
      421 tccgctggat tttgactatg tgcatgtgtc gcgctacggc gacaaaaccc gtggcggcga
      481 gctggtgtgg cgtcaggccc ccaaagaaga cgtgcgcgac cgcgtggtgc tggtgctgga
      541 cgatattctg gacgaaggcg aaaccatggc cgccattcgc gacaaggtgc tggccatggg
      601 cgccaagtct ttccatagcg ccgtgttcgc caacaagctg atcagcaagg ccaagccgat
      661 tgctgctgat tttgtcggca tcgatgtgcc ggatcgctat gtgtttggct acggcatgga
      721 tgtgcgcggc gcctggcgca atctgccggc gatttacgcg ctgaaataat gctggcctat
      781 cgccttgtgg tgctgccggc tggcggcttg gctgacaacg ggtgatgggg ctgtttccgc
      841 ggcctgatta cccctggtgc ccgctgagcg gccatgccac cccgccctga ccgatgtcac
      901 accatgcgca gggcattttc cgggcttgct gcgggcccgt ccggtcggtg acaatacccg
      961 tcttatgcca gcccgcctag cccgccattt actccgtcac caccgcacgc cagccgccaa
     1021 tctgcaactg ggcggcatgc tggcctggat tgccggcgct gccaacgcca gcggttttct
     1081 ggtggtgcat caatatactt cccacatgac cggcatggtg tccagcctgg ccgatgcgct
     1141 ggcgctgggc cagttaagcc tggcctcctc cacgctgatt tcgattgcct gttttgtgct
     1201 gggcagcgcc tgctgtgcgc tgtgcgtgca atgggcgcgg cggcgggtgc gccatgaata
     1261 tgcgctggcc ttgctgatcc aggcggtgct gatgctgttg tttggtttgt tggccggtcg
     1321 ggtggcgcca ggcgtatcgc taaccttgca catgacggcc tggtcgctgt gcctgatgat
     1381 gggctggcaa aacgccatga tcaccaagct ctcgcatgcg gaaatccgca ccacgcatgt
     1441 tactggtctg ctcactgaca tcggcattgg cctggggcgc tggctggggc cggcgcagga
     1501 ccgcctgacg cgtcggcggg cgcggcgcaa gctggctgtg caggcggcga ttgttggtct
     1561 gttttttatc ggtgggctgt gcggggcatg gacatttcag tgggtgggcg tggtggccag
     1621 tgtgccgctg gcgctgctgc tgatcgccat ggcgctggcg ccattgtggg acgactggcg
     1681 tgcctggcat cgctggcagc gcatgcaccg cgtcaactga gctttttcct ggagcattca
     1741 tgattgcaat cattggcggc agcagtttgt cgcaactgcc cggcctggcc atcacccacc
     1801 gccaggtggt gcgcacgccg tatggcgagc catcctgtgc cttgaccttt ggcctgctgg
     1861 ctggccgcac ggtggtgttt ctggcgcggc acggccatgg tcacatgcac gccccacacg
     1921 aaatcaacta ccgtgccaat ttatgggcgc tgaaatcaca gcaagtgcgc catgtgctgg
     1981 ccatcggctc ggtggggggc attcatctgg ggctggcgcc gggcgccctg gccattccgg
     2041 atcagttgct tgactatact tggggacgca agcacacctt ttttgaaggg ccggagtcgc
     2101 cggtggtgca cgtagacttc acccagccgt actctgccac attgcgccag tgtttgctgg
     2161 atggcgcgca gcatgccggt gttgaggcgc tggacggcgg cacctatgcc tgcacgcaag
     2221 gcccccggct ggaaaccgcc gctgaaatcg cccgcctgcg ccgcgacggc gctgacatgg
     2281 tgggcatgac cggcatgcca gaggcggcct tggcgcgcga gctggacatg gaatacgcct
     2341 gtctggcggt ggtggccaat tatgctgccg gttgtggcga cagcatcggc aaggtggatt
     2401 tcaccgccgc cggtgcggtg atgggcgagg ccatggacaa agtagcgcgc gtgctggaag
     2461 ccagcctggc cctgggggcg catctggggc attgagtgcc ggacctgggc gatgggccca
     2521 gccggtaagg agaccaatat gtcggttcga agcattctga aaatgggcga tgtgcgcctc
     2581 ttgcaacagg cgcgcccggt ggaggatttc accgcgccct ggctggccac gctgctggcg
     2641 gatctgcacg acaccatggc cgagcatcat ggcgctggct tggctgcgcc gcagataggt
     2701 gagccgtggc aggtggtggt atttggcagc ggcgcgccca atccgcgcta tcccgatgcc
     2761 gagccggtgc cggccacggt gttgatcaac cccattctga cgccactggg ggcggccatg
     2821 gaagaagact gggagggctg tttgtccatt cccggcctgc gtggggtggt gcctcgctac
     2881 cgcgagttgc gttatcaggg atttgatgcc catggccagc cgattgaccg caccgtatcg
     2941 ggatttcatg cccgggtggt gcagcatgaa tgtgaccatc tgtggggcat gctctacccg
     3001 atgcgcatga aagacatgag ccagctgggc tttactgacg tgctgtttcc cgagctggcc
     3061 ctggtggccg cagccgaacc gcgctgagcg gccggcctgc cgccattaca ctctgcgccg
     3121 cacttagcgc cgctcgcaaa acccagcgag cgctcctggc tagcacgacc gtagacagta
     3181 caagcagtac gacaaggtcg tgcaacgacg ccaggagggt tttgtaagcg gcgcttatgc
     3241 cgccgcttgc agttgcgcct gattggcggc gtgggcgtcg gtggcaaagt ggcgcaccag
     3301 attgcccatt tcgtccgctt tgaccgagag atcatgaatc aggtcgccag cgctggcgat
     3361 ttcgcgggta gaggcctcaa tgcggccact gatggtttgc atgcgctcag ccaactcgcg
     3421 cgcagcggcg gattgctggg tgttggtgct ggcaatgctg cgcatcatgt ggtccacctc
     3481 ggcgctggcg tgcaggattt cgtccaggct gtgcgcggtg tcgctcaggt gttgttcgcc
     3541 ctgagtggtg cgctcgccga cctgactcat gctggctacg gcagaatccg tcactgcccg
     3601 caccctggcg accaactggt tgatttcgct agtgctgtgg ccggtgcgct cggccagttt
     3661 gcgcacttcg tcggccacca cggcaaatcc ccgtccttgt tcgccagcgc gagccgcctc
     3721 aattgccgca ttcagcgcca gcagattggt ctggtcggca atctcgcgaa tggtgtcggt
     3781 caccacgcca atatgggtga cggcttgata cagctcctgg atggtggcct gggcttcttc
     3841 caccgtgcgt gccgccgcgc gggtttcctc gcaggattgc gccatctggt tggcgccagc
     3901 gccag
//
LOCUS       DAOPYX010000278         3884 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_30908_length_3884_cov_14.565161, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000278 DAOPYX010000000
VERSION     DAOPYX010000278.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 3884)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3884)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3884
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30908_length_3884_cov_14.565161"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1097)
                     /locus_tag="PKN55_12030"
     CDS             complement(<1..1097)
                     /locus_tag="PKN55_12030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008952087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor protein PhoR"
                     /protein_id="HNX72019.1"
                     /translation="MLRSLYWLAGVGAAAALAALLFGWAVGLALALLAALIGLGFHLY
                     YLDKLLAWLDNPAPERTPEGMGGWQDVFSALYRQSRQQTMAQKKLSSALERFINAGEA
                     MPDGVVVLDEHDRIEWLNPMAVDHFQLDRKRDVGNMVANLVRQPGFHDYLLAQNYSQP
                     LILRLSQPVEQVLAVQLVPFDSTRKLLISRDITQLDRVQTVHRDFVANVSHELRTPLT
                     VIGGFLETLADMDAPEPDVTRQFLPMMMEQAKRMQSLVEDLLTLSRLENGSKTASQEK
                     VDMAALLNTLRVEAEGLSQGRHQIILGDCDAANLWGNANELHSAFGNLVSNAVRYTPE
                     GGRIRLDWRLDKGRGVFSVSDTGIGIAPQHIP"
     gene            complement(1266..1955)
                     /gene="phoB"
                     /locus_tag="PKN55_12035"
     CDS             complement(1266..1955)
                     /gene="phoB"
                     /locus_tag="PKN55_12035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004283175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon transcriptional regulator
                     PhoB"
                     /protein_id="HNX72020.1"
                     /translation="MPANILLVEDEPAIQELIAFNLTQAGHHVLRAASAENAMTLVKN
                     ALPDLILLDWMLPGASGVEFAKKLRADERTRGVPIIMLTARSDEQDKITGLETGADDY
                     ITKPFSPRELQARIKAVLRRRAPQMTDDAVDINGLRLDPVTHRVHGHGQPIDLGPTEF
                     RLLHFFMTHPERVHSRAQLLDQVWGDHVFVEERTVDVHIRRLRSALEPSQHDSLIQTV
                     RGTGYRFSAQP"
     gene            2116..2394
                     /locus_tag="PKN55_12040"
     CDS             2116..2394
                     /locus_tag="PKN55_12040"
                     /inference="COORDINATES: protein motif:HMM:NF019214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase N-terminal
                     domain-containing protein"
                     /protein_id="HNX72021.1"
                     /translation="MLPDTPLETLWLAIGLLGQALFAGRFIWQWWVSERLGRSVVPVA
                     FWHLSLVGGAILTVYAVYRQDVVFALGQGCGLAVYVRNLQLIRRQGVL"
     gene            complement(2402..3280)
                     /gene="yedA"
                     /locus_tag="PKN55_12045"
     CDS             complement(2402..3280)
                     /gene="yedA"
                     /locus_tag="PKN55_12045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012698561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HNX72022.1"
                     /translation="MFVAVPAARWRLLAAFAALYVIWGSTYLAIRIGVASWPPLMMAA
                     VRFLLAGGLLYAWQRGRGAPAPSRRQWLHAGAIGALLLAGGNGGVTVAEQWVASGVAA
                     LAVATVPLFTLACAQMFGQRASAREWFGIALGLAGMLLLNLGANLQASPLGAALLLFA
                     SASWAFGSVWGKRLDLPAGPMAAATEMLVGGAVLALASALAGERPQAWPTLEGWLALA
                     YLVVFGSLVAFSAYLYLLKTVRPALATSYAYVNPVVAVGLGVAFAGERIGSAEWLAMA
                     VIVLAVVWISLPRRAA"
     gene            3454..>3884
                     /locus_tag="PKN55_12050"
     CDS             3454..>3884
                     /locus_tag="PKN55_12050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014088654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HNX72023.1"
                     /translation="MELDKFDRLIVESLHHNARLSGAELARRVNLSAPAVAERLHKLE
                     RAGVIRGYQAVLDPEALGSPIQCLIELTLARADYLHAQNTLAGLPELLECHRVTGDAC
                     MVLKAAVRSMSHLQALIERLSSLGSTKTSIILSSPFSGRMPP"
BASE COUNT          699 a         1270 c         1255 g          660 t
ORIGIN      
        1 gggatgtgtt gcggggcgat gccgatgccg gtgtcgctga cgctgaacac gccgcgcccc
       61 ttgtccagcc gccagtccag gcggatgcgc ccgccttccg gggtgtagcg cacggcgttg
      121 gacaccagat tgccaaaggc gctgtgcagt tcgttggcgt tgccccacag attggcggcg
      181 tcgcaatcgc ccaggatgat ctgatggcgg ccttgcgaca ggccttcggc ttccacgcgc
      241 agggtgttga gcagggcggc catgtccact ttttcctggc tggcggtttt gctgccgttt
      301 tccaggcgcg acagggtgag caggtcttcc accaggcttt gcatgcgctt ggcctgttcc
      361 atcatcatcg gcagaaactg gcgggtgacg tccggttcgg gggcgtccat atcggccagg
      421 gtttccagaa agccgccaat caccgtcagc ggggtgcgca gttcgtgcga gacattggcg
      481 acaaagtcgc ggtgcacggt ctgcacgcgg tccagctggg tgatgtcgcg cgagatcaac
      541 agcttgcggg tggagtcaaa cggcaccaac tgcaccgcca gcacttgctc caccggctgg
      601 ctgagccgca ggatcagcgg ctggctgtag ttttgcgcca gcaggtagtc atgaaagccg
      661 ggctggcgca ccagattggc caccatattg cccacgtcgc gcttgcggtc cagctgaaag
      721 tggtcaaccg ccatcgggtt gagccattcg atgcggtcgt gctcgtccag caccaccacg
      781 ccatcgggca tggcttcgcc ggcgttgatg aagcgctcca gcgcgctgct gagttttttc
      841 tgcgccatgg tttgctggcg gctttgccgg tacagcgcag aaaacacatc ctgccaaccg
      901 cccatgcctt ccggggtgcg ctcgggcgcg gggttgtcca gccaggccag cagcttgtcc
      961 aggtaataca gatgaaagcc caggccaatc agcgccgcca gcagcgccag cgccaggccg
     1021 acagcccagc caaacagcag cgccgccagc gccgctgccg cgccaacgcc ggccagccag
     1081 tacagactac gaagcaaaag gggacgcatg cacatcctta cgcaaagcaa aaacgaatca
     1141 tgcctgaatt ttgtctggca ggcggggtgg gcgagcggcg ccgccgcatg tcgcgcggcc
     1201 cacaaaaccc agcgcagcac gccaggaagg tgtggtgagc cgcgcggtag gccacggcgc
     1261 gccgtttacg gctgggcaga aaaacgatag ccggtgccgc gcacggtctg gatcaggctg
     1321 tcgtgctggg acggctccag cgcgctgcgc aggcggcgga tgtgcacgtc caccgtgcgt
     1381 tcttccacaa acacatggtc gccccacact tgatccagca gctgcgcgcg cgagtgcacg
     1441 cgctcggggt gggtcatgaa gaaatgcagc aggcggaatt cggtcggccc caggtcaatc
     1501 ggctggccgt ggccgtgcac gcggtgggtt accggatcca ggcgcaggcc gttgatgtcc
     1561 accgcgtcgt cggtcatctg cggcgcgcgg cggcgcagca cggccttgat gcgcgcttgc
     1621 agctcgcgcg gcgaaaacgg cttggtgatg tagtcatcgg cgccggtttc cagcccggtg
     1681 attttgtcct gctcgtccga gcgggcggtg agcatgatga tgggcacgcc gcgcgtgcgc
     1741 tcgtcggcgc gcagtttttt ggcaaattcc acgcccgagg cgcccggcag catccagtcc
     1801 agcaggatca ggtcgggcag ggcgtttttc accagcgtca tggcgttttc cgccgaggcg
     1861 gcgcgcagca cgtggtggcc ggcctgggtc agattgaagg caatcagttc ctggatggcc
     1921 ggctcgtctt cgaccagcag aatattggcg ggcatggcgg gtttccgttg tcgcgtttga
     1981 agaaattatg gcaaggatag aaacttgctg ttacggaaac atgacaagtc cggccttcgc
     2041 tgattttatc cttgtcatcg ctgcgtcaag cgcgccgcgc acactggcca tccctgcctt
     2101 tttgccggag ctgcgatgtt gcccgacacc ccgctggaaa ccctgtggct ggccatcggc
     2161 ctgctcggtc aggccttgtt tgccgggcgt ttcatctggc aatggtgggt cagcgagcgg
     2221 ctggggcgca gcgtggtgcc ggtggcgttc tggcatttat cgctggttgg cggcgccatt
     2281 ctcaccgtgt atgcggtgta ccggcaggat gtggtgtttg ccctgggcca gggctgcggg
     2341 ctggcggtgt atgtgcgcaa tttgcagctg atccgccgcc agggggtttt gtgagcgact
     2401 ctcaggccgc ccgccggggc aggctgatcc acaccaccgc cagcacaatc accgccatcg
     2461 ccagccattc ggccgagccg atgcgctcgc cggcaaacgc cacgcccagc cccaccgcca
     2521 ccaccgggtt cacataggca tagctggtgg ccagcgccgg gcgcacggtt ttcaagagat
     2581 aaagataggc cgaaaacgcc accagcgaac caaacaccac cagataggcc agcgccagcc
     2641 agccttccag cgtgggccag gcctgcggcc gttcgcccgc cagcgcgctg gccagcgcca
     2701 gcaccgcgcc gcccaccagc atttcagtgg cggccgccat cggcccggcc ggcaaatcca
     2761 gccgcttgcc ccacaccgag ccaaacgccc agctggccga ggcaaacagc aacagcgccg
     2821 cgcccagcgg gctggcttgc agattggcgc ccagattcag caacagcatg ccggccaggc
     2881 ccagggcaat gccaaaccac tcgcgcgccg aggcgcgctg gccaaacatc tgcgcacagg
     2941 ccagggtaaa cagcggcacg gtggccaccg ccagcgccgc cacgccagag gccacccatt
     3001 gctcggccac cgtcaccccg ccattgccgc cagccagcaa caaggcgcca atcgcgcccg
     3061 cgtgcagcca ttgccgccgg ctgggcgcgg gcgcgccgcg tccgcgctgc caggcgtaca
     3121 acagcccgcc ggccagcaaa aagcggactg ccgccatcat cagcggcggc cagctggcca
     3181 cgccaatgcg gatggccaga taggtggagc cccaaatcac atacagcgcg gcaaacgccg
     3241 ccagcaagcg ccagcgcgca gcgggaacag caacaaacat ggcaggtctt tcagtcaggg
     3301 cggcgccttc gcatccgtca cccagacata taagaaaagc aaatattgta aaagcccgcc
     3361 ctgcgctatc aacaccacaa aaccctgctt gtggccgggt tctgatttat aaaatggggt
     3421 tttttgcccc tgctgctaaa gaatcgaaag ctgatggaac tggataaatt cgaccgcctg
     3481 attgtggagt ccctgcatca caacgcccgc ctgtccggcg ccgaactggc ccggcgggtg
     3541 aacctttcgg ccccggcggt ggccgagcgc ctgcacaagc tggagcgcgc cggggtgatt
     3601 cgcggctatc aggccgtgct cgaccccgaa gcgctgggca gcccgattca gtgcctgatc
     3661 gagctcaccc tggcgcgggc ggattatctg cacgcgcaaa acacgctggc cggcttgccc
     3721 gagctgctgg aatgccatcg ggtgacgggc gacgcctgca tggtgctcaa ggcggcggtg
     3781 cgctcgatgt cgcatctgca agcgctgatc gagcgattaa gcagcctggg cagcaccaaa
     3841 acctcgatta ttttgtccag cccgtttagt gggcgcatgc cgcc
//
LOCUS       DAOPYX010000279         3877 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_30994_length_3877_cov_7.396389, whole genome shotgun sequence.
ACCESSION   DAOPYX010000279 DAOPYX010000000
VERSION     DAOPYX010000279.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 3877)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3877)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3877
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30994_length_3877_cov_7.396389"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(45..1205)
                     /locus_tag="PKN55_12055"
     CDS             complement(45..1205)
                     /locus_tag="PKN55_12055"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNX72024.1"
                     /translation="MTTLPPTVWLLALSQALAMTVVSLTLSASAWVSSKLAGPDWATL
                     PLAAQYLATLLLLSPVAWAMTRLGRRPVLAAGALLGMLGLALAALAIARQQFAWFVAA
                     SGLVGAFNAVAQYYRFVAADVAPPGQHGRAIAATLTGGVLAAWLGPALASHTRLLGPQ
                     PFTVSFVLLAALAGLAALCALAMRIPPGALPASGARRPWRTHAGNPRLWLAVLAASSG
                     YALMNLLMTATPLAMRCAQFDFAAAATVIQWHIVAMFAPSLLSGRLVQRLGAPGVMLG
                     GAVLMLASVGVSLRGETFGHFQWALALLGVGWNGVYVGASSLLTECCWPAEKAWIQAG
                     NDSMVFLGVTLMTAAAAPAVNAWGWMTLNQLALAPLLLLAGACGWLYWRPPA"
     gene            complement(1202..1444)
                     /locus_tag="PKN55_12060"
     CDS             complement(1202..1444)
                     /locus_tag="PKN55_12060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX72025.1"
                     /translation="MMNAVDLAAVLPTTLTAANTPVGLSGEIISHSHIGQEFAWPPED
                     PRQARQTQREYMASHAKRVVDAQGLTQFYEPVGGQG"
     gene            1515..2510
                     /locus_tag="PKN55_12065"
     CDS             1515..2510
                     /locus_tag="PKN55_12065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011134868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlxA family transcriptional regulator"
                     /protein_id="HNX72026.1"
                     /translation="MSRYSAGVNDINPPLSAMTRHLLFFVFADFQLLDVSGPMAAFEM
                     ANRIAPGSYRWTVCSQTGGQVTASCGVALDSQPLAASTCVDTLLAPGGDGVERACDCV
                     ATRVWLKEITQTARRVASVCSGAYLLAASGLLDGRRATTHWRRETDFVRRFPAVQLEA
                     DRIYIQDDKLWTSAGITAGIDLSLALIADDLGEAVARQVARQLVVYYRRPGGQSQYSA
                     LLDMAPAQGRFAGVLRHMREHLHLALNVEALAEVARMSPRHFARCFRAELGMTPARAL
                     ERLRIEAARAALESGALSVKSVARDYGFADPERMRRAFVRVLGISPRALQLREAG"
     gene            complement(2487..3284)
                     /locus_tag="PKN55_12070"
     CDS             complement(2487..3284)
                     /locus_tag="PKN55_12070"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNX72027.1"
                     /translation="MVEFVSLTHGGQPVSIEYQWLNREREHTPLLVFLHEGLGSVSMW
                     RDYPQTLCDAAGCRGLVYSRPGYGRSTPREPEARWQPDYLHIQSWQVLPALLDALGVE
                     APCWLFGHSDGGSMALLFAARFAERTAGIITLAPHIFVEEITVAGIAAAREAYLAGGL
                     RQGLARHHADPDSPFWGWNHAWLNPAFRQWNLEADIVGIRCPVLAIQGEGDEYASMAQ
                     IDGIQRIVPHTHLLKLADCGHSPHRDQAAAVTEATLAFLRSASFAQL"
     gene            complement(3356..>3877)
                     /locus_tag="PKN55_12075"
     CDS             complement(3356..>3877)
                     /locus_tag="PKN55_12075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008954266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HNX72028.1"
                     /translation="AILALLTAVGGGVLRDVLIQRTPRILYQNDTLWVIAATLALAWG
                     LRLQTRRAPQLERLFILADSIGLVAFSIGGAQIGAMLGLSAFGVAMLGFISAVGGGMI
                     RDMLVNDIPFILHRDFYGAVSILVALGWHALNQLGWQRPETEYALFALGLALRLWAHR
                     QELRLPAPDASGH"
BASE COUNT          699 a         1286 c         1269 g          623 t
ORIGIN      
        1 catctgcaag gctgcaccat cacgtcaggc agataaccct gtgttcaggc tggcggccgc
       61 cagtacagcc agccacacgc cccggccagc agcaagagcg gggccaacgc cagctggttt
      121 aatgtcatcc agccccaggc attgactgcc ggcgcagcgg cggcggtcat cagggtgacg
      181 cccagaaaca ccatgctgtc gttgccggcc tgtatccagg ctttttcagc tggccagcag
      241 cattcagtca acaggctgct ggcccccaca tataccccgt tccagcctac gcccagcaag
      301 gccagcgccc actgaaaatg gccaaacgtt tcgccgcgca ggctgacccc cacgctggcc
      361 agcatcaaca ccgccccgcc cagcatcacc cctggtgccc ccaggcgctg caccagtcgg
      421 ccgctgagca aagagggggc aaacatggcg acgatatgcc actggatcac cgtggcggcg
      481 gcggcaaaat caaactgcgc gcagcgcatg gccagcggcg tggcggtcat cagcagattc
      541 atcagcgcat agccactgct ggcggccagc accgccaacc acagccgtgg gttgcccgca
      601 tgggtgcgcc agggccggcg tgcgccgctg gcgggcagcg cgccgggggg gatgcgcatg
      661 gccagggcgc acagcgccgc cagcccggcc agcgccgcca acaacacaaa gctgaccgta
      721 aagggttgtg gccccagcag acgggtgtga ctggccagcg ccgggcccag ccaggccgcc
      781 agcacaccgc cggtcaacgt ggcagcaatc gcgcgcccgt gttggccggg gggcgccacg
      841 tccgccgcga caaagcggta atactgcgcc acggcattga atgctcccac caagccactg
      901 gcggccacaa accaggcaaa ctgctgccgg gcaatcgcca gcgccgccag cgccaggccc
      961 agcatgccca gcagcgcacc cgccgccaac actggccgtc gccccagccg cgtcatcgcc
     1021 caggccacgg gcgacagcaa cagcaaggtg gccaggtatt gcgccgccag cggcaaggtg
     1081 gcccagtctg gcccggccag cttgctgctc acccaggccg aggcggacag cgtcaggctg
     1141 accacggtca tcgccagcgc ctggctgagc gccagcaacc agacggtggg cggcagcgtc
     1201 gtcatccctg gccaccgacg ggttcataaa actgtgtcag cccttgtgca tccaccaccc
     1261 gtttggcatg acttgccatg tattcgcgct gcgtttgcct ggcctggcgc ggatcttccg
     1321 gcggccaggc gaattcctga ccaatatggg aatgggagat gatttctcca ctcaagccta
     1381 ccggggtatt ggcggcagtc aaggtggtgg gcaggacagc ggcaagatcc acagcgttca
     1441 tcatggggtt cgactcctga aagcggttca cggttgacat atcacaacgc acatcaagtc
     1501 ttgcgatcgg cataatgagc cgctacagtg ccggggtaaa tgacattaat ccccctcttt
     1561 ctgccatgac ccgtcacctg ctgttttttg tgtttgccga ctttcaactg ctggatgtgt
     1621 ctggcccgat ggcggcgttc gagatggcca atcgcatcgc gccgggcagc tatcgctgga
     1681 cggtatgcag tcagactggc ggacaggtga cggcttcctg cggggtggcg ctggacagcc
     1741 agccattggc ggcaagcacc tgcgtggata ccttgctggc gccgggtggc gatggggtgg
     1801 agcgcgcctg tgactgtgtg gcgacccggg tctggctgaa agagatcacc cagacggcgc
     1861 ggcgggtggc cagcgtgtgt tctggcgcgt atttgctggc ggccagcggc ttgctggatg
     1921 gccgccgcgc caccacccac tggcggcgcg agacggattt tgtccggcgc tttccggctg
     1981 tgcagctgga ggccgaccgg atttatattc aggacgacaa gctatggaca tcggccggca
     2041 tcaccgccgg cattgatttg tcgctggcgc tgattgctga cgatctgggt gaggcggtgg
     2101 cgcggcaggt ggcgcgccag ttggtggtgt attaccggcg accgggcggg caatcgcagt
     2161 attctgcctt gctggacatg gcgccggcac aggggcgctt tgccggggtg ctgcgccata
     2221 tgcgcgagca tttgcatctg gccttgaatg tcgaggcgct ggccgaggtg gcgcgcatga
     2281 gcccgcgcca ctttgcccgc tgctttcgcg ccgaattggg catgacgccg gcgcgggcgc
     2341 tggagcggct gcgcattgaa gcggcgcgcg ccgcactgga aagcggcgcg ttgtcggtca
     2401 aatccgtggc gcgcgattac ggctttgccg accccgaacg catgcgccgc gcctttgtgc
     2461 gcgtgctggg catctcccca cgcgccttac aactgcgcga agctggctga acgcaagaaa
     2521 gccagcgtgg cctcggtgac ggcggcggct tggtcacgat gcgggctgtg gccgcagtcg
     2581 gccagcttga gcaggtgggt gtgcggcaca atgcgctgga tgccgtcgat ctgcgccatg
     2641 ctggcgtatt cgtcgccctc gccctgaatg gccagcaccg gacagcggat gccgacaata
     2701 tcggcctcca gattccattg ccggaacgcc ggattcagcc aggcgtggtt ccagccccag
     2761 aacggcgaat ccgggtcggc gtggtggcgg gccaggccct ggcgcaggcc gccggccaga
     2821 taggcctcgc gcgcggcggc gatgccggcc acggtgatct cttcgacaaa aatatgcggg
     2881 gccagggtga tgatgccggc ggtgcgctca gcaaagcgcg cggcaaacag caaggccatc
     2941 gaaccgccgt cgctgtggcc aaacagccag cacggcgcct ctacgcccag cgcatccaat
     3001 agcgccggca gcacctgcca gctctggatg tgcagataat ccggctgcca gcgcgcctcg
     3061 ggctcacgcg gggtcgagcg gccatagccg gggcgcgaat acaccaggcc acgacagccg
     3121 gcggcgtcgc acagcgtttg cggatagtcg cgccacatgc tgaccgagcc taaaccctcg
     3181 tgcagaaaca ccaagagcgg cgtgtgttca cgctcgcggt tcagccattg gtattcgatg
     3241 ctgaccggct ggccgccgtg cgtcagcgag acaaattcca ccatgtgctt ttttcccttg
     3301 cttaaagctg ctcaccaagc tctgctggcg ttgttgcgcg gccttgtcgt gccgctcagt
     3361 gcccgctggc gtccggcgcg ggcaagcgca gttcttgccg gtgcgcccac agccgcagcg
     3421 ccaggcccag cgcaaacagc gcgtattcgg tttcgggccg ttgccagccc agttgattca
     3481 gcgcatgcca gcccagcgcc accagaatgg acaccgcgcc gtaaaaatcc cggtgcagga
     3541 taaacgggat gtcattgacc agcatgtcgc ggatcatgcc gccgcccacc gcgctgataa
     3601 agcccagcat ggccacgcca aaggcgctta agcccagcat ggcgccgatt tgcgcgccgc
     3661 cgatggaaaa cgccaccagg ccaattgaat cggccaggat gaacagccgc tccaactgcg
     3721 gcgcgcgccg ggtttgcaag cgcaagcccc aggccagcgc cagcgtggcg gcaatcaccc
     3781 acagcgtgtc gttctggtaa agaatgcgcg gggtgcgctg gatcagcaca tcgcgcagca
     3841 cgccgccgcc aacggcggta agcaaggcca gaatggc
//
LOCUS       DAOPYX010000280         3872 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_31047_length_3872_cov_11.673566, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000280 DAOPYX010000000
VERSION     DAOPYX010000280.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 3872)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3872)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3872
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31047_length_3872_cov_11.673566"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..530
                     /locus_tag="PKN55_12080"
     CDS             <1..530
                     /locus_tag="PKN55_12080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018152679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HNX72029.1"
                     /translation="EELPGLPHQDYTPFAAPHGDDPRAWAQFNALYTSLSEPGAALAA
                     ESQLIAYLAAHGFARLDAAPPMRPADPALRQAHARIMDQLAEPLSLGELAAVAGLSRY
                     QLIRGFKHAYGQTPHAYQLDQRINRAKRLLKRGERIADVAQQLGFADQSHFQRHFKQR
                     LATTPWHYQQCLRRA"
     gene            complement(540..1133)
                     /locus_tag="PKN55_12085"
     CDS             complement(540..1133)
                     /locus_tag="PKN55_12085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018150346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine phosphorylase"
                     /protein_id="HNX72030.1"
                     /translation="MSITVLFPTATEASLFQHPRVQTVISGVGLTATAHQTSKLIAQH
                     RPDWLILAGIAGVYPHAGLAVGEVALVASELEGDLGFFTPQGFTHLAHLPLDMDFARR
                     HTLHCPYVDAVSGFTLARGVSLNAAMAPFIDTSAVDIENMEGAAFFHVCLQENMRFLQ
                     LRAISNVALPGEDDWDMAGSVQALTDGLHRLLAQLGA"
     gene            complement(1189..1842)
                     /gene="nfsB"
                     /locus_tag="PKN55_12090"
     CDS             complement(1189..1842)
                     /gene="nfsB"
                     /locus_tag="PKN55_12090"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003743542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HNX72031.1"
                     /translation="MDIARIATTRYTSKAFDPSRKIPAAQIEQLKTLLRYSPSSTNSQ
                     PWHFVLASTEAGKAKVAESTSGFYVFNAAKVQNASHVVVLCARSEMTDAHLARLLAQE
                     EQDGRFATPEAKAGQNNGRTYFANMHRFDLKDAQHWMEKQVYLALGTLLLGAAALEID
                     ACPIEGFDVKLLNDALDLRGKGFTAVALVALGYRAADDFNAALPKSRLPMDEVLTEI"
     gene            1960..2865
                     /locus_tag="PKN55_12095"
     CDS             1960..2865
                     /locus_tag="PKN55_12095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019921947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HNX72032.1"
                     /translation="MRGLQDLQILVRTAERGSVSAAARELGVSPAVASLAIKRLEDEL
                     GCVLCLRSTRSLRLTQHGERFVAHARQMLASYQQALAELHPQAAPLREVLHLSMPSDF
                     GRNLALGWLDDFQRAHPHVQLRLSLSDRLADMYREPVDLAIRFGLPPDSNLVALPLAE
                     CRRVLCAAPSYLARHGAPGCPQALAEHNCLCFIVGERLHNRWRFWRDGGEWVQQVAGD
                     RSADDADVVRRWAVAGHGLVYKSQLDLAPDLAAGRLLRVCPEWQGEPAPLYLLCADRR
                     QLSPTVRQLREFLRERCLALLAGRL"
     gene            complement(2917..3399)
                     /locus_tag="PKN55_12100"
     CDS             complement(2917..3399)
                     /locus_tag="PKN55_12100"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNX72033.1"
                     /translation="MGAEAQTADFACGEPVLDQYLQRYARQDIKRGVARVFVATAEPQ
                     PAEVLGFYTLSAASIAADTLPENWRKRLPRYPVPVALLGRLAVHQRHHGQGLGAILLA
                     DACQRVAAASATLAVAALVVDAKSPQAAAFYRHFGFIELPGQAGRWMLPRAHFMSFCA
                     "
     gene            complement(3420..3719)
                     /locus_tag="PKN55_12105"
     CDS             complement(3420..3719)
                     /locus_tag="PKN55_12105"
                     /inference="COORDINATES: protein motif:HMM:NF020265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HNX72034.1"
                     /translation="MINLRITVMPTLAKDARLHIRCDQEMRHLLDKAAAYAHMSVSEF
                     VLNHALAQALSVVQAHEAISLSPQDFAAFLDALDAPSAGNAALQRAFVRHAKHVV"
BASE COUNT          730 a         1284 c         1210 g          648 t
ORIGIN      
        1 aagaagaatt acccggcctg ccgcatcagg actacacccc gtttgccgcg ccgcacggcg
       61 acgatccgcg cgcctgggcg cagttcaacg cgctgtacac cagcctcagc gagccgggcg
      121 cggcgctggc ggccgaaagc cagttgattg cgtatttggc cgcgcatggc tttgcccgcc
      181 tggatgccgc gccgccgatg cgcccggctg acccggcctt gcgtcaggcg cacgcgcgca
      241 tcatggatca gttggccgag cccttgtcgc tgggcgagct ggcggcggtg gccgggctca
      301 gccgctatca gctgattcgc ggctttaaac acgcttatgg acaaaccccg cacgcctacc
      361 agctggatca gcgcatcaac cgcgccaaac gcctgctcaa gcgcggcgag cgcatcgccg
      421 acgtggcgca gcaactgggt tttgctgatc agagccattt tcagcggcac ttcaaacaac
      481 ggctggccac cacgccctgg cattatcagc agtgtttgcg gcgggcgtag cggcccacgc
      541 tacgccccca gttgggccag cagccggtgc aaaccgtcgg tcagtgcctg caccgagccg
      601 gccatgtccc aatcgtcctc gccgggcagc gccacattgg aaatggcccg caactgcaaa
      661 aagcgcatgt tttcttgtaa acacacatga aaaaacgccg cgccttccat gttttcaatg
      721 tccacggcag acgtatcaat aaagggcgcc atggcggcgt tcagcgacac accgcgcgcc
      781 agggtaaacc cgctgacggc gtccacatac gggcaatgca gggtgtggcg gcgggcaaag
      841 tccatgtcca gcggcaaatg cgccagatgg gtaaagccct gcggggtgaa aaaccccaaa
      901 tcgccctcca gctctgaagc caccagcgcc acctcgccca ccgccagccc ggcgtgcggg
      961 tacaccccgg caatgccggc caaaatcaac cagtccgggc ggtgttgcgc aatcagcttg
     1021 ctggtttgat gggcggtggc ggtgagccca acgccagaaa tcaccgtctg cacgcgcggg
     1081 tgctgaaaca ggctggcctc ggtggcggtc gggaacagga cggtgatgga catgggcgca
     1141 gggcagtcgg ttagggaggc cgacagtgta cctgcgccgg cgcggcgatc aaatctcggt
     1201 cagcacctcg tccatcggca ggcgcgactt gggcagggcg gcgttaaagt catccgccgc
     1261 gcgatagccc agcgccacca gcgccacggc ggtaaagccc ttgccgcgca ggtccagcgc
     1321 atcgttcagc agtttgacgt caaagccttc aatcgggcag gcgtcgattt ccagcgcggc
     1381 ggcgcccagc agcagcgtgc ccagcgccag atagacttgt ttttccatcc agtgctgggc
     1441 gtctttcagg tcaaagcggt gcatattggc aaagtaggtg cggccgttgt tctggccggc
     1501 cttggcctcg ggcgtggcaa agcggccgtc ttgctcttcc tgcgccagca ggcgcgccag
     1561 atgggcatcg gtcatctcgc tgcgcgcgca cagcaccacc acatgcgagg cgttttgcac
     1621 cttggcggcg ttgaacacat aaaacccgct ggtggactcg gccaccttgg ccttgccggc
     1681 ctcagtgctg gccagcacaa agtgccaggg ctgcgaattg gtggacgacg ggctgtagcg
     1741 cagcagggtt ttcagctgct cgatctgcgc ggccggaatc ttgcggctgg ggtcgaaggc
     1801 tttgctggtg tagcgggtgg tggcgatgcg ggcgatgtcc atggcgggcc tctcacgaaa
     1861 tggcaaattg acaacaaaaa acacgaccct cagtgtgccc gcttttactt tgcagataaa
     1921 cttgccaatc agcaattcac tttcaacagg aaattgataa tgcgcggcct gcaagacctg
     1981 caaatcctgg tgcgcaccgc cgagcgcggc agcgtctccg ccgctgcgcg cgagctgggc
     2041 gtgtcgcccg ccgtggccag cctggccatc aagcggctgg aagacgagct gggctgtgtg
     2101 ctgtgcctgc gctccacccg cagcctgcgg ctgacccagc acggcgaacg ctttgtcgcc
     2161 cacgcccgcc agatgctggc cagctatcag caagcgctgg ccgagctgca cccgcaagcc
     2221 gcgccgctgc gcgaagtgct gcacctgtcg atgccgtccg actttggccg caacctggcg
     2281 ctgggctggc tggacgactt tcagcgcgcc cacccgcatg tgcagctgcg cctgagcctg
     2341 tctgaccggc tggccgatat gtaccgcgag ccggtggacc tggccatccg ctttggcctg
     2401 ccgccggatt ccaacctggt ggcgctgccg ctggccgaat gccggcgcgt gctgtgcgcc
     2461 gcgcccagct acctggcccg ccacggcgcg ccgggctgcc cgcaggcgct ggccgagcat
     2521 aactgcctgt gttttatcgt cggcgaacgc ctgcataacc gctggcgctt ttggcgcgac
     2581 ggcggcgagt gggtgcagca agtggccggc gaccgcagcg ccgacgacgc cgacgtggtg
     2641 cgccgctggg cggtggccgg ccacggcctg gtgtataaat cccagcttga ccttgccccc
     2701 gacctggccg ccggccggct gctgcgggta tgcccggaat ggcaaggcga acccgcgccg
     2761 ctttacctgc tgtgcgccga ccgccgccaa ctctcgccca ccgtgcggca gctgcgcgaa
     2821 tttttgcgcg aacgctgcct ggcgctgctg gcggggcggc tgtgagcgtg cgggatgccc
     2881 gcaggggccg tcagggagat ttttcagggt atctgttcag gcgcaaaacg acatgaaatg
     2941 cgcgcgcggc agcatccagc gcccagcttg gccaggcaac tcgatgaagc caaagtgccg
     3001 atagaatgcg gcagcctgtg gggatttggc atctaccacc aaggcggcga cagccagcgt
     3061 ggcactggca gccgccacac gctgacaagc atccgccagc aaaatagccc ccaacccctg
     3121 gccatgatgc cgctgatgca cagccaaacg gccaagtaaa gccaccggca ccgggtagcg
     3181 cggcagccgc tttcgccagt tttcaggaag cgtatcggcg gcaatgctgg ccgcactgag
     3241 cgtataaaag cccagtacct cagccggctg aggctcagcc gtggccacaa acacccgagc
     3301 cacaccacgc ttgatgtctt ggcgggcata gcgttgcaag tattggtcga gcacaggctc
     3361 accgcaggca aaatctgccg tctgcgcctc ggcgcccaat gcgccaatct ggtagttcat
     3421 cacaccacat gcttggcgtg gcggacaaac gcacgctgca aagcagcatt gcccgcagag
     3481 ggagcatcca gcgcatccag aaacgcggca aaatcttgtg gcgacaaact gatcgcctcg
     3541 tgcgcctgca ccactgacag agcctgcgca agcgcatggt taagcacaaa ctcagacaca
     3601 ctcatatgcg catacgccgc cgccttgtcc agcaagtggc gcatttcctg gtcacagcga
     3661 atgtgcagac gagcatcttt agcaagggtg ggcatgaccg ttatcctgag gttgatcaag
     3721 ccaattgtgc gccatatgta cgcacaccag caagcccccc gctcttttag acgcggctaa
     3781 caaaactccg cgcagcgatc ctggctagtc gccagtcacg ctgaaacacg agggctcatg
     3841 cgccacggag aacccctcgc cgcaccacac cg
//
LOCUS       DAOPYX010000281         3858 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_31201_length_3858_cov_8.111491, whole genome shotgun sequence.
ACCESSION   DAOPYX010000281 DAOPYX010000000
VERSION     DAOPYX010000281.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 3858)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3858)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3858
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31201_length_3858_cov_8.111491"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..556)
                     /locus_tag="PKN55_12110"
     CDS             complement(<1..556)
                     /locus_tag="PKN55_12110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008952860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HNX72035.1"
                     /translation="MSDALTAVAGEAARRRTFAIISHPDAGKTTLTEKLLLFSGAIQM
                     AGTVKGKKSGKFATSDWMEIEKQRGISVASSVMQFDYREHVVNLLDTPGHQDFSEDTY
                     RVLTAVDAALMVIDAAKGVEAQTIKLLNVCRLRTTPIVTFMNKYDREVRDSLELLDEV
                     ENVLNIRCAPITWPIGMGKTFRGVY"
     gene            746..1369
                     /locus_tag="PKN55_12115"
     CDS             746..1369
                     /locus_tag="PKN55_12115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011135934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase subunit alpha"
                     /protein_id="HNX72036.1"
                     /translation="MFTGIVAGTAPVVAIDAREKFQTHIVELPLDWTDGLEPGASVAH
                     NGCCLTVTAVDGARVSFDLMQETLACTNLGDIKTGDRVNIERAARFGAEIGGHAMSGH
                     VLCTAEVIEVIDTPNNRTTWFRLPDDYARFLFTKGYIGVDGCSLTIGAVRGSEFCVHL
                     IPETLARTTLGWRAPGQRVNIEIDPQTQVIVDTVERLLVERGLIAGG"
     gene            1490..2092
                     /locus_tag="PKN55_12120"
     CDS             1490..2092
                     /locus_tag="PKN55_12120"
                     /inference="COORDINATES: protein motif:HMM:NF013933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HNX72037.1"
                     /translation="MTVSLSWMLPLATYMAVMSITPGPNNVMLTASGVAFGYRRTLPH
                     LLGVSLGHSAQIGLVCLGLGSVFVRWPWLHQLLAWAGGAYLLWMAWRMLGAGGAGEAA
                     LQRPQSFWQAAGFQWINPKAWVMATSVASLFLPPDWPLLAGAAWVTLVAVVVNYPCVS
                     VWTLFGAMLRRALTEPARLRQFNQLMAALLVGTAAMLVWR"
     gene            2274..3695
                     /locus_tag="PKN55_12125"
     CDS             2274..3695
                     /locus_tag="PKN55_12125"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HNX72038.1"
                     /translation="MFTLDPTLPATEQIVNQAQAAIDSGRLGEGARLPSVRQLAARLE
                     VSVFTVVNAYDRLLARGVIRSRVGAGYFVCRRPGIGAPALVAAADPQLLPNTALGLVQ
                     GVLGGSRYKVPAGSGFLPEAWLEDTLAPAVLTRALRRQPFASQPAPMQGHPAAREQIA
                     LRLAQLQLPIPHEQLLITHGATQAFDLVLRACLRPGDSVLVEDPGYFMIHAQLRALGM
                     QVLPVPRRADGPDLDTLEALAREHRPRLLFTQTLLHNPTGGNTSPQVAHRLLMLAERH
                     DFLVLEDDVYGELASQPGMRLAQIDGLNRVFYIGSFSKTLNPGLRLGYVAAPKTWLDK
                     LIELKVMSTLSGSALNEAVITDILESGYYRRHTERLRERLARARRVSIAALTECGVTL
                     PHADGEGVFLWAQLPDGLHADAITRRAADAGILFAPGTLFSPSGGGQQHLRLHAAYAS
                     DPAVLACLREGLDALGEKRLRLA"
BASE COUNT          636 a         1302 c         1216 g          704 t
ORIGIN      
        1 gatacacgcc acggaaggtc ttgcccatgc caatcggcca ggtgatcggc gcgcaacgga
       61 tgttgagcac gttttcgact tcgtccaaca gctccagcga atcgcgcact tcccggtcgt
      121 acttgttcat gaaggtcaca atcggcgtgg tgcgcagccg gcagacattc agcagcttga
      181 tggtttgcgc ttctacgccc ttggcggcgt caatcaccat caaggcggcg tccactgcgg
      241 tcagcacgcg ataggtgtct tccgagaagt cctggtggcc tggggtgtcc agcaggttca
      301 ccacatgctc gcggtagtca aactgcatca ccgacgaggc caccgaaatg ccgcgctgct
      361 tttcgatttc catccagtcg gaggtggcga acttgccgct tttcttgccc ttgaccgtgc
      421 cggccatctg gatggcgccg gagaacagca gcagtttttc ggtaagcgtg gttttacccg
      481 catcagggtg agaaataatg gcgaaagtgc ggcggcgcgc ggcttcgccg gccacggcgg
      541 tcaaggcgtc ggacatggga gggccaggga aagaaaaagc cttgaattgt aacggagata
      601 gccggtcagc aaaaccccgc gccagcgtgc cgaccaaatt gccgccgcca aagcgacgca
      661 cctgtgcctc ctgctgcatt gcgccatcgg cacagcgaag cgagtacaat cctttaaccc
      721 caccctttgt cagaaaggca tgcccatgtt taccggaatc gtcgccggca ccgcgccggt
      781 ggtggccatt gatgctcgcg aaaaatttca gacccatatt gtcgagctgc cgctggactg
      841 gaccgacggc ctggagcccg gcgcctcggt ggcgcacaac ggctgctgcc tgaccgtcac
      901 cgccgtggac ggcgcacggg tgagctttga cctgatgcag gaaaccctgg cctgcaccaa
      961 tctgggcgac atcaaaaccg gcgaccgggt gaacatcgag cgcgccgccc gctttggcgc
     1021 cgaaatcggc ggccacgcca tgagcggcca tgtgctgtgc accgccgaag tgattgaggt
     1081 gatcgacacc ccgaacaacc gcaccacctg gttccgcctg cccgacgact acgcgcgctt
     1141 tttgttcacc aagggctata tcggtgtgga cggatgcagc ctgaccatcg gcgcagtgcg
     1201 cggcagcgag ttttgcgtgc acctgatccc ggaaaccctg gcccgcacca ccctgggctg
     1261 gcgcgccccc ggccagcggg tgaatatcga aatcgacccg caaacccagg tgattgtgga
     1321 caccgtcgag cgcttgctgg tggaacgtgg tttgattgcc gggggctgaa tccgccccaa
     1381 ctgtattgtt ttttctgtca cacagtctgc gctcactgtc actgtccgcg ctggcctgcg
     1441 ccccctagca tgggggcgtg ttcttcatgg cttgcaaagg actgatgcaa tgactgtctc
     1501 gctttcctgg atgttgccgc tggccaccta tatggcggtg atgtcgatca cccccggccc
     1561 gaataatgtc atgctgaccg cctcgggcgt ggcgtttggc tatcgccgca ccctgccgca
     1621 tctgctgggc gtcagcctgg gccattcggc acaaattggc ctggtgtgcc tgggcctggg
     1681 cagcgtgttt gtgcgctggc cctggctgca ccaactgctg gcctgggctg gcggcgcgta
     1741 tctgctgtgg atggcctggc gcatgctggg cgcgggcggc gcgggtgagg cggccttgca
     1801 gcggccgcaa agcttttggc aagcagccgg gtttcagtgg atcaacccca aggcctgggt
     1861 gatggccacc tcggtggcca gcctgttcct gccgccggac tggcccttgc tggccggcgc
     1921 cgcctgggtg accctggtgg ccgtggtggt gaactacccc tgcgtgtcgg tgtggacctt
     1981 gtttggcgcc atgctgcgcc gggcgctgac cgagccggca cggctgcggc aattcaacca
     2041 actgatggcc gccctgctgg tgggcaccgc cgcgatgctg gtgtggcgtt agcgccgcgc
     2101 accccctggc gttgttgccc gaccttgccg taccccttcc tggcgcgcct ggctttgcta
     2161 tcctgctgaa tattgtctgc ggccgcgcgc ttggctgggt ttgccgcgcg acgcttgcgc
     2221 cactccgccc ccccgcccgc cgcccgacgg gctgctgaat acacgagaaa gccatgttca
     2281 ccctggaccc caccctgccc gccaccgaac aaatcgtcaa ccaggcgcaa gccgccatcg
     2341 acagcggccg gctgggcgaa ggcgcgcggc tgccttcggt gcgccagctg gcggcgcggc
     2401 tggaagtcag cgtgttcacc gtggtcaacg cctacgaccg cctgctggcg cgcggggtga
     2461 tccgctcgcg ggtgggcgcc gggtattttg tgtgccgccg gcccggcatt ggcgcgccgg
     2521 cgctggtggc cgccgccgac ccgcaattgc tgcccaacac cgcgctgggc ctggtgcagg
     2581 gcgtgctggg cggcagccgc tataaagtgc cggccggctc gggctttttg cccgaagcct
     2641 ggctggaaga caccctggcc ccggccgtgc tcacccgcgc cctgcgccgc cagccgtttg
     2701 ccagccaacc cgccccgatg caaggccacc cggccgcgcg cgagcaaatt gccctgcgcc
     2761 tggcgcaact gcaactgccc atcccgcacg aacaactgct gatcacccac ggcgccaccc
     2821 aggcctttga cctggtgctg cgcgcctgcc tgcgccccgg cgacagcgtg ctggtggaag
     2881 accccggcta tttcatgatc cacgcgcaac tgcgcgcgtt gggcatgcag gtgctgccgg
     2941 tgccgcgtcg cgccgacggc cctgacttgg acaccctgga agccctggcc cgcgaacacc
     3001 gcccgcgtct gttgttcacg caaaccctgc tgcacaaccc cactggcggc aacacctcgc
     3061 cgcaagtggc gcaccgcctg ctgatgctgg ccgaacgcca tgattttttg gtgctggaag
     3121 acgatgtgta tggcgaactg gccagccagc ccggcatgcg cctggcgcaa atcgacggcc
     3181 tgaaccgggt gttttacatc ggcagcttct ccaaaaccct caaccccggc ctgcgcctgg
     3241 gctatgtggc cgcgccaaaa acctggctgg acaagctgat tgaactgaaa gtgatgagca
     3301 ccctgtccgg ctcggcgctg aacgaagcgg tgatcaccga catcctggaa agcggctact
     3361 accgccgcca caccgaacgc ctgcgcgaac gcctggcccg cgcccgccgc gtcagcatcg
     3421 ccgccctcac cgaatgcggc gtcaccctgc cgcacgccga cggcgaaggc gtgttcctgt
     3481 gggcgcaact gcccgacggc ctgcacgccg acgccatcac ccgccgcgcc gccgacgccg
     3541 gcatcctgtt cgcccccggc acgctgttct cccccagcgg cggcggccaa caacacctgc
     3601 gcctgcacgc cgcctacgcc agtgatccgg cagtgctggc gtgcttgcgg gaggggttgg
     3661 acgcactggg ggagaagcgg ttgcggttgg cgtgaggggg acgcgcttgc tatgtaaagt
     3721 gggtgggatt taaaatgaca aagaagacag caaagtcatt ggcacttatt catcgaatgg
     3781 tcgtatttgg cacgcattgc taaggacgca ctgatttatt ccccaacagc tggcccgaag
     3841 cctcctgatc tgcgaaca
//
LOCUS       DAOPYX010000282         3858 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_31202_length_3858_cov_7.360242, whole genome shotgun sequence.
ACCESSION   DAOPYX010000282 DAOPYX010000000
VERSION     DAOPYX010000282.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 3858)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3858)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3858
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31202_length_3858_cov_7.360242"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            95..595
                     /locus_tag="PKN55_12130"
     CDS             95..595
                     /locus_tag="PKN55_12130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX72039.1"
                     /translation="MIRHISWLGASLLALGTACVQADPLAFPASLQGKAIELAPNDPD
                     YARAAVEYAVKPAGQGGVSAALVVKITQDIPVYRMWNGPGDVNAQGNTNRLGGWWSYD
                     APHGPVDGYRVAYEICRDWNKLKWVATCTLKAGPAATRPAPSNTRPTRWIGRRLSINR
                     GRAPAS"
     gene            complement(777..2810)
                     /locus_tag="PKN55_12135"
     CDS             complement(777..2810)
                     /locus_tag="PKN55_12135"
                     /inference="COORDINATES: protein motif:HMM:NF028510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cache 3/Cache 2 fusion domain-containing
                     protein"
                     /protein_id="HNX72040.1"
                     /translation="MSYRTMPLGRQILLPAMLLLVLVFTIMIALTAWLTGQAAMAQAQ
                     LELGNEVRLVVGGLDSEFDSAKARGARQLHFFEQFLGGAVQPGEGLHPTGGLALPILS
                     VNGVMLNSHHEPLERFKSLTSEEAAILTVYQGKVYRLSTLLKRDGKAMDGTALADNDP
                     VARALLAGEDYQGMVVRNGKYYFSNIRALKNAQGQVIGGLSVRIELADTLARLRTLYG
                     RIVAGKTGYVTIARPSGDDKTIGEFIVHPKFQGKIIGEVVGEGPTRQSALDNIRFEGG
                     LRQYLWPSEQGLRERMAMVGWSKNWNFQVTIGSWTDEFMADSVHLRGILAAVSLGGLA
                     LCSVLIWLLVRHRLRPLRGIVDAVTALGQGNLQVSVPQRRTDSHNELDQLGQALNTTV
                     GQVRSLLSDITTAAGQVGESAARLDTRSEELVRRAGTQAQAAAAMAASVEQLSVSIRQ
                     VADHAHTASTLSAEALHSCQTGRSVVTGSADDMRAMADDVRQTASAVLALGEQSRLIS
                     SVVEVIHDIADQTNLLALNAAIEAARAGESGRGFAVVADEVRKLAERTTASTREIAAT
                     IQQIVHDTQHAAQRMQVVRERVDHGVELACQAGVALDTIDQHTASAVQLVHGIAGSTQ
                     EQRGAGDTLTHGIDDIARMAEAASEMARDHAEDVERLRQVAGQLRAGLARFTL"
     gene            complement(3268..>3858)
                     /locus_tag="PKN55_12140"
     CDS             complement(3268..>3858)
                     /locus_tag="PKN55_12140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008953869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005488 - binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RHO alpha subunit C-terminal catalytic
                     domain-containing protein"
                     /protein_id="HNX72041.1"
                     /translation="YNFNWKTFIEVYSEDYHVDPFHPGLGNFVNCGELKWEWGEQYHV
                     QTVGVKNALARSGSKVYSEWHQQVLRRYAEKQPEFGAIWLTYYPNIMVEWYPHVLVVS
                     VAVPKGPEKTTVITEFYYPEDIVWFEPEFIQAEQAAYFETAVEDDEICYRMHEGRKAL
                     WQRGESEVGPYQSPTEDGMQHFHEFYRRKMGEALRG"
BASE COUNT          743 a         1226 c         1149 g          740 t
ORIGIN      
        1 gacctttgct ggtcaggatg gcgcgcgtct tggcggggag gtctttcacg ctaagagcgg
       61 ccaacacccg ttttcttgat gaaggaagca caccatgatt cgacacatat cctggctggg
      121 cgccagcctg ctggcgctgg gcactgcctg cgttcaggcc gacccgctgg cctttccggc
      181 cagcctgcaa ggcaaggcaa tagaactggc ccccaatgat ccggactacg cccgcgcggc
      241 ggtggaatac gccgtcaagc cggccggcca gggcggtgta tctgccgcgc tggtggtcaa
      301 aatcacccag gatatcccgg tgtaccggat gtggaatggc ccaggcgacg tcaacgccca
      361 gggcaatacc aaccgcctgg gcggctggtg gtcgtatgat gccccgcacg gcccggtgga
      421 cggctaccgg gtagcgtatg aaatctgccg cgactggaac aagctcaagt gggtggccac
      481 ctgcacactg aaagcgggtc ctgcggcgac ccgaccggcg ccgagcaata cccggccaac
      541 ccggtggatt ggcagacgtt tgtcaataaa ccgtgggcgc gcaccagcga gctgagctgt
      601 ccgccggaaa gcgccgacta tcaggcagat ccgctggaca tcgccaaacc caagccctga
      661 tagcacgcag gcaccccggc agtgccctgg cttgctcaag ccaccgtacg gcactgccgg
      721 cacatctgga ttgccattgg ccggcatgaa tatgtaatgc gggcattccg gttttgctac
      781 aaggtaaagc gcgccaagcc cgcgcgcaat tgtccggcca cctgacgcag gcgctcgaca
      841 tcctcagcat ggtcacgagc catctcgctg gcggcttctg ccatgcgggc aatatcatca
      901 atgccgtggg taagggtatc gccagcaccg cgctgttctt gcgtgctgcc ggcgatgccg
      961 tgcaccagtt gcacggcact ggccgtgtgt tggtcgatgg tatccagcgc caccccggcc
     1021 tgacaggcca gttccacgcc gtgatccacc cgctcgcgca ccacttgcat acgctgggcg
     1081 gcgtgctggg tgtcatgcac aatctgctgg atggtggcgg caatttcccg ggtagaagcg
     1141 gtagtgcgct cggccagctt gcgcacttca tcagccacca cggcaaaacc ccgaccggat
     1201 tccccggcac gggctgcctc gatggcggca ttcaaggcca gcagattggt ctggtcggca
     1261 atgtcgtgaa tcacctccac cacactggaa atcagccgcg actgctcgcc cagtgccagc
     1321 acggcgctgg cagtctgccg gacatcgtcg gccatggcgc gcatatcgtc agcactgccg
     1381 gtgaccacac tgcgcccggt ctggcagctg tgcagcgcct cggcactgag ggtcgaagca
     1441 gtatgggcat gatcggccac ctgacggatg ctgaccgaca gttgttctac cgaggccgcc
     1501 attgccgcgg cggcctgtgc ctgtgtgccg gcacgacgca ccagctcttc cgaacgggtg
     1561 tccagcctgg cggcggattc gccaacctgg ccggcggccg tggtgatgtc gctgagcagg
     1621 ctgcgcacct ggccgaccgt ggtgttcagc gcctggccca gttgatccag ttcattgtgg
     1681 ctgtcggtgc gtcgctgcgg cacactgact tgcagatttc cctggccaag cgccgtgacc
     1741 gcatcgacaa tcccacgcaa gggtcgcagc cggtggcgca ccagcagcca gatcaacacc
     1801 gagcacaagg ccaggccacc caggctgacc gcagccagaa tgccacgcaa atgtacgctg
     1861 tcggccatga attcgtcggt ccagctgccg atggtgacct gaaaattcca gttttttgac
     1921 caacccacca tggccatgcg ctcgcgcagc ccctgctcgg acggccacag atactggcgc
     1981 agcccaccct caaaacggat attgtccaac gcggattgcc gggttgggcc ttcgcccacc
     2041 acctcgccga taatcttgcc ctgaaatttg gggtggacaa taaactcgcc aatggtcttg
     2101 tcgtcgccac tggggcgagc aatggtcaca tagccggtct tgccggcaac aatgcggcca
     2161 tacagggtgc gcaggcgcgc cagggtgtcg gccagctcta tgcgcaccga cagcccgcca
     2221 atcacctggc cctgggcatt tttcagcgca cggatattgc tgaagtaata cttgccattg
     2281 cgcaccacca tgccttgata atcctcgccg gccagcaatg ccctggctac cggatcgttg
     2341 tcggccagcg cggtgccgtc catggctttg ccgtcacgct tgagcagcgt ggacaagcgg
     2401 taaaccttgc cttggtatac ggtcagaatg gcggcttctt cgctggtgag gctcttgaaa
     2461 cgctccagcg gctcgtggtg gctgttgagc atcacgccat tcacgctcag gatcggcagg
     2521 gccagtccgc ctgtggggtg cagcccttca cctggctgta ccgccccacc cagaaactgc
     2581 tcgaaaaaat gcagttggcg ggcgccgcgt gccttggcgc tgtcaaactc gctgtccagc
     2641 cctcccacca ccagccgaac ctcattgccc agctccagct gtgcctgcgc catggccgcc
     2701 tggccagtca gccaggccgt cagcgcaatc atgatggtaa aaaccaggac cagcagcagc
     2761 atggccggca acaggatttg ccgacccagg ggcatggtgc gatacgacat ggtgcgatct
     2821 cctttgtcgt gggcgcatcg ctgactgcgt gcgcgacaat cacacctcgg tgaggtgtga
     2881 tggctgtcag ccttggcata tcaagaaagg tggaacttgg ttttgccaga cccgtgcggc
     2941 aggtcgcagc atgacaattt atgaaaataa catgaacaga gggataaaaa aaggcttatc
     3001 acaccagaca gaaatgtttc acttgaacac gccaacaagg cagacaaaaa agccgcaggt
     3061 cgttgatatg gatggcagga tgatcgcccc cagccaataa gagccgctca caaaaccccc
     3121 tggcgttgtg gtgtgaccgt actgtctgcg gtcacacgcc tagccaggac cgctacgctg
     3181 ggttttgtga gcgaccctaa aaacgccacg gcgcgccgga cattcatccg gcgcgccgtg
     3241 gcggggtgtt tcagctgctg ttttggctta gccgcgcagc gcttcgccca tcttgcggcg
     3301 ataaaactca tggaagtgct gcatgccgtc ttcggtcggc gactgatacg ggccgacttc
     3361 tgattcaccg cgctgccaca gcgccttgcg gccctcgtgc atgcggtagc agatttcgtc
     3421 gtcctcgacg gcggtttcga agtacgccgc ctgctcggcc tggatgaatt ccggctcgaa
     3481 ccagacgatg tcttccgggt aataaaactc ggtgatcacc gtggtttttt ccgggccctt
     3541 gggcaccgcc acgctgacca ccagcacatg cggataccac tccaccatga tattggggta
     3601 gtaggtcagc cagatggcgc cgaattccgg ctgtttttct gcgtagcggc gcagcacctg
     3661 ctggtgccat tccgagtaca ccttgctgcc cgagcgcgcc agggcgtttt ttacgccgac
     3721 ggtctgcaca tggtattgct cgccccattc ccacttcagt tcgccgcaat tgacgaaatt
     3781 gcccaggccg gggtggaagg ggtcaacgtg gtaatcctcc gaatacactt cgataaaggt
     3841 tttccagttg aagttgta
//
LOCUS       DAOPYX010000283         3845 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_31361_length_3845_cov_10.845910, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000283 DAOPYX010000000
VERSION     DAOPYX010000283.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 3845)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3845)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3845
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31361_length_3845_cov_10.845910"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            177..818
                     /locus_tag="PKN55_12145"
     CDS             177..818
                     /locus_tag="PKN55_12145"
                     /inference="COORDINATES: protein motif:HMM:NF019960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HNX72042.1"
                     /translation="MRRTKAEAEQTRNDLLDAAEQLFDQRGVSSTSLADIAKAAGVTR
                     GAVYWHFTDKEDLLKSMCERTCLPFDHLTEQLLAELPGGALAALRRHYLAVFEEVVRS
                     PRTRQVFNILWNKYECPDDNSPVMQRRRQHLDEEHQLLTTVLRAAREQGELRHPGELD
                     DLAVILQTVVMGLIELWLADPNRFAIETRSRAYVDILMGVLGVAPEGEPKPKS"
     gene            complement(998..1219)
                     /locus_tag="PKN55_12150"
     CDS             complement(998..1219)
                     /locus_tag="PKN55_12150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX72043.1"
                     /translation="MPMSYEQHLDEVTTLITEKYDIADQPAIDMVMRAQADGYFSGHD
                     DHPAICTLDRAHEDARTVFKKYGKGKKVN"
     gene            complement(1210..1461)
                     /locus_tag="PKN55_12155"
     CDS             complement(1210..1461)
                     /locus_tag="PKN55_12155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VF530 family protein"
                     /protein_id="HNX72044.1"
                     /translation="MNTQAHNPLHGVTLAQMLTELVEECGWVTLGQEIPIRCFTHEPS
                     LASSLKFLRRTPWAREKVESFYLFIQRQKARDGKRPPCP"
     gene            1634..2476
                     /locus_tag="PKN55_12160"
     CDS             1634..2476
                     /locus_tag="PKN55_12160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011137912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HNX72045.1"
                     /translation="MPASRDERAVYFRADDIAPLECLDAVYTRQVFAPHFHEEYVVNT
                     LTAGAQSCGCHGSSHVAGVGALVLINPGETHTGAAAHAQGWAYCGMYPAASLLQRLAC
                     EMAGRAVTPYFRETVIVDPALSRELVGLHRLLREVADPLTRESALYTVFGQVLRRHMS
                     VREQAPALAHTAVERARQILADRLAEPVSLQELAEAVGLSPWHLSRSFRQRFGLPPVA
                     WRNQLRVARAKVWLAQGRAVSEVASQLGFADQPHLTRAFRATLGVTPGGYQRGLRGAA
                     QPAG"
     gene            2579..3289
                     /locus_tag="PKN55_12165"
     CDS             2579..3289
                     /locus_tag="PKN55_12165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011137913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HNX72046.1"
                     /translation="MAFRAGVFWQGVRDTTPMLVGAAPFGVIFGALAVSAGLSAWGAL
                     ALSGVVFAGSSQFVAVSLMAAGAALPVIWLTTLVVNLRHALYSAALLPHAQGLSARWR
                     WCLAFWLTDETFAVVEHRLRTQGMGADGQWFWLGSSLAMYLNWLGWTLVGVVLGQNIP
                     GLESWGLDFAMAATFTAIVTPQLARRPVLAAAVCAGVTALLARGLPYKLGLMLAAAAG
                     VLGGMLAERGLARQELPS"
     gene            3286..3606
                     /locus_tag="PKN55_12170"
     CDS             3286..3606
                     /locus_tag="PKN55_12170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008954684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HNX72047.1"
                     /translation="MKLDLLWTVLGMLAVTYSVRVSFLGFGQRVRFPAWLQRSLHYVP
                     AAVLTALVAPMTLAPQGALDVSLSNAYLPGVLATGLAAWWTRHTLGAIVCGFVVYAGW
                     RWLG"
BASE COUNT          632 a         1127 c         1319 g          767 t
ORIGIN      
        1 cccaatctta catacatgct agaatgtatg taaatacaaa tttgcgacct cctaccccgt
       61 cgccacgcca tgtgcttgtt cccatggcat aaatcgcccg gatcgcccca gcactgccga
      121 ttcatgcgac aatccgcgcc catcgcgcca gacccaccca acccagagcc cacgccatgc
      181 gccgcaccaa agccgaagcc gaacaaaccc gcaacgacct gctggacgcc gccgaacagc
      241 tgttcgacca gcgcggcgtg tccagcacct cgctggccga catcgccaag gccgccgggg
      301 tcacgcgcgg cgcggtgtac tggcacttca ccgacaaaga agacctgctc aaatccatgt
      361 gcgaacgcac ctgcctgccg ttcgaccacc tcaccgagca actgctggcc gaactgcccg
      421 gcggcgcgct ggcggcgctg cgccgccact acctggcggt gtttgaagaa gtggtgcgct
      481 caccgcgcac ccggcaggtg ttcaatattc tgtggaataa atacgaatgc ccggatgaca
      541 actcgccagt gatgcaacgc cgccgccagc atctggacga agaacaccaa ttgctcacca
      601 ccgtgctgcg cgccgcgcgc gaacaaggcg aactgcgcca ccctggcgaa ctggacgacc
      661 tggcggtgat tctgcaaacc gtggtgatgg ggctgattga actgtggctg gccgacccga
      721 accgctttgc cattgaaacc cgctcgcggg cgtatgtgga tatcttgatg ggggtgttgg
      781 gggtggcccc ggaaggcgag ccgaagccta agagttaagc gatagcggcg accgacgcga
      841 aggcaaataa cggctaacaa aactgtcccg acattgctgt gcgatctggc caggatcgct
      901 tcgccggatt tggtgagcca tcctcaccca ggaaaatctg gccagtgtgg cgccgtgcac
      961 gcaggaggga tggccgtgcg cgcagcagcg cctgatctca attcaccttc ttgcccttgc
     1021 catacttctt gaacaccgtg cgcgcgtcct catgggcgcg gtccagcgtg cagatggccg
     1081 ggtggtcgtc gtggccgctg aaatacccgt cggcctgggc gcgcatcacc atgtcgatgg
     1141 cgggctggtc ggcgatgtcg tatttttcgg tgatcagcgt ggtgacttca tcaaggtgtt
     1201 gctcgtagct catgggcatg gggggcgctt tccgtcgcgc gccttctggc gctggatgaa
     1261 taaataaaac gattccactt tttcccgcgc ccagggcgtg cggcgcaaga atttcaggct
     1321 ggacgccagg ctgggctcgt gggtaaagca gcggatgggg atttcctggc cgagcgtgac
     1381 ccaaccgcac tcttcaacaa gttcggtgag catctgcgcc agggtcacgc catgcagcgg
     1441 gttatgggct tgcgtgttca gggtgtgcct ctatcaacaa tcaaggcccg ccgcctcatc
     1501 ggcgggccgc agcgcgtatt gtggcatgag gcggacagat gttcagcgct tttgcgcagg
     1561 gcaaaatcct tcaagccggg ctggcgggcg gcgtgccaca atctgccctt gcgccccctc
     1621 ccccggaacc ccgatgcctg cctctcgcga tgaacgcgcg gtgtatttcc gcgccgacga
     1681 cattgccccg ctggaatgcc tggatgcggt ttacacccgc caggtgttcg ccccgcattt
     1741 tcatgaagaa tacgtagtca acacgctgac cgcaggcgca caaagctgtg gctgccacgg
     1801 cagctcgcat gtggccggcg tgggtgcgct ggtgctgatc aaccccggcg agacgcacac
     1861 tggcgcggcg gcgcatgcgc agggctgggc gtattgcggc atgtacccgg ccgccagctt
     1921 gttgcagcgc ctggcctgcg agatggccgg gcgggcggtg acgccgtatt ttcgggagac
     1981 ggtgattgtc gatccggcct tgtcgcgcga gctggtgggc ttgcatcggc tgttgcgcga
     2041 ggtggccgac ccgctgacgc gcgaatcggc cttatatacg gtgtttggcc aggtcttgcg
     2101 ccggcatatg agcgtgcgcg agcaagcgcc ggccctggcg cacacggcgg tggagcgggc
     2161 gcggcaaatc ctggccgacc gcctggccga gccggtgtcc ttgcaggaat tggccgaggc
     2221 ggtggggctg tcgccatggc atttaagccg cagttttcgc cagcgctttg gcttgccgcc
     2281 cgtggcctgg cgcaatcagc tgcgggtggc gcgcgccaag gtgtggctgg cgcaggggcg
     2341 ggcggtcagc gaggtggcca gtcagttggg ttttgctgac caaccgcatc tgacacgggc
     2401 gtttcgcgcc acgctggggg tgacgccggg gggttatcag cgtgggttgc ggggggctgc
     2461 gcagcccgca ggataataag cctttttgcc ggcacccgcc ggctatgcgc aagatcattc
     2521 aagctttttt cagcggatgg cggcaagata gcggccgggt tttgcacagg gagcaaacat
     2581 ggcttttcgg gccggggtgt tttggcaggg cgtgcgcgac accacgccga tgctggtggg
     2641 ggcggcgccg tttggggtga tttttggcgc actggcggtc agcgccgggc tgtcggcctg
     2701 gggggcgctg gctttgtcgg gggtggtgtt tgccggttct tcgcagtttg ttgccgtcag
     2761 tttgatggcc gccggggctg cgctgccggt gatctggctg accaccttgg tggtgaattt
     2821 gcgccacgcc ctgtatagcg cggccttgct gccgcatgcg caaggattat cggcgcgctg
     2881 gcgctggtgc ctggctttct ggctgaccga cgaaaccttt gccgtggtcg agcaccgctt
     2941 gcgcacgcag ggcatgggcg cggatgggca gtggttttgg ctgggctcgt cgctggccat
     3001 gtatctgaac tggctgggct ggacgctggt gggggtggtg ttggggcaga atattcccgg
     3061 actggaatcc tgggggctgg attttgccat ggcggcgact tttaccgcga ttgtcacccc
     3121 gcagctggcg cgccggccgg tgctggcggc agccgtgtgc gccggggtga ccgccttgct
     3181 ggcgcgaggg ttgccgtaca agctgggcct gatgctggcg gcggcggccg gggtgttggg
     3241 cggcatgctg gccgagcgtg ggctggccag gcaggagctg ccatcatgaa actggattta
     3301 ctgtggacgg tgctgggcat gctggcggtg acttacagcg tgcgggtgag ttttttgggg
     3361 tttggccagc gcgtgcgctt tccggcgtgg ttgcagcgca gcttgcatta tgtgccggcg
     3421 gcggtgctga ccgccttggt ggcgccgatg acgctggcgc cgcagggcgc gctggatgtg
     3481 tcgctgagca atgcctattt gccgggcgtg ctggctaccg ggctggcggc atggtggacg
     3541 cggcacacgc tgggggcgat tgtctgcggt tttgtggtgt atgccggctg gcgctggctg
     3601 ggctgagcgc acataaaaaa actccctttg gcgcatgcca tcgggagtca acacgaggaa
     3661 gaaacaacac gggaggtgcc tgccacacgc agacactgca atcctgtgaa agcctgtgcg
     3721 caccatgatt gtgcaagtga acaggcattc atataacgta aatatactag tcctgtgcgc
     3781 tgcaacacgg ccaatcataa aaactgattg ggggcgccaa gttctgctta taaccgctgt
     3841 attaa
//
LOCUS       DAOPYX010000284         3838 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_31455_length_3838_cov_12.148295, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000284 DAOPYX010000000
VERSION     DAOPYX010000284.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 3838)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3838)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3838
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31455_length_3838_cov_12.148295"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            108..554
                     /locus_tag="PKN55_12175"
     CDS             108..554
                     /locus_tag="PKN55_12175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003770159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HNX72048.1"
                     /translation="MSLKARINDDMKAAMKAREAERLATIRLLLAAMKQKEVDERIEL
                     DDAAIIAVVDKMLKQRRDSVTQYEAANRQDLADRERFEMTVLADYLPQPLSEAEIDAL
                     IEAAVASTGAAGLPDMGKVMGAVKGQMAGRADMAAVSARIKARLTR"
     gene            574..2331
                     /gene="dnaG"
                     /locus_tag="PKN55_12180"
     CDS             574..2331
                     /gene="dnaG"
                     /locus_tag="PKN55_12180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019389345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HNX72049.1"
                     /translation="MIPQDFIDQLLTRVDIVDVVERYVPLKKAGQNYMACCPFHKEKS
                     PSFSVSPSKQFYHCFGCGAHGNAIRFVMEHAGLGFIDAVNELAGSVGMSVPQAPASPQ
                     QQQAARQRPSLLECMGQAARFYKQSLKRSPEAIAYLKKRGLTGEIAARFALGYAPDGW
                     TPLQQAFDDYLDDNLVECGLVLRHDDGRRYDRFRHRIMFPIRSSRGDIIGFGGRVLDQ
                     GEPKYLNSPETPLFEKGRELYGLYEARQGIRDAGRVLVVEGYMDVVALAQYGIDYAVA
                     ALGTATTPTHVQKLLRQADHVFYCFDGDAAGQRAAWRALENSLEALVDGKALHFLFLP
                     SEHDPDSYVREFGRDAFEQALTRDSLPLSAYLVNELTRRVDMQSQEGRAELVKLATPL
                     LAKVGAPTLGVMLRRRVAELAQIDLNELDRLTGRTPRFGKKTHPRGGARGPRHDPSLV
                     RRLIRWLALNPVLAAQVQLDIDPAECGPELQALLALALLIQSEATPPSPAQLEERLRD
                     TELGERLRPLLVQAWQDGDLADALPQEVSDASRRLADNIRHAQLERLTEKERHQGLTD
                     EEKRRLLELLRMSAGPSRS"
     gene            2383..>3838
                     /gene="rpoD"
                     /locus_tag="PKN55_12185"
     CDS             2383..>3838
                     /gene="rpoD"
                     /locus_tag="PKN55_12185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014088321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HNX72050.1"
                     /translation="MAATPENDKDVEIKAQTLADNADERRKRLKQLIVLGKERGYLTY
                     AEINDHLPEDVLDAEQIESIIGMITDMGIQVCDEAPDAEQLLMSDATPAVADEDAVEE
                     AEAALSTVDSEFGRTTDPVRMYMREMGTVELLTREGEIEIAKRIEDGLKHMIQAISAC
                     PGTVAEVLALIDRIEAGEMRVDELIDGFIDPDAPEEETAEFAEPSDEELLADEEDDEE
                     EDEDGEAAAAVNNANLEELKAQTLEHFGVVKALYVKMLKALEKSGSQSKAYLEQQEAI
                     TAEFMKVRFSVKQVEALCESLRSSVDEIRRFEREIMDICEQKAKMPHARFIETFPGSE
                     NDPAWVEREIAVGKPYSNALERFKYAIIEKQSKLGELQQKAMIPIKELKDINKQMALG
                     ESKARMAKREMIEANLRLVISIAKKYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYK
                     FSTYATWWIRQAITRSIADQARTIRIPVHMIETIN"
BASE COUNT          832 a         1189 c         1200 g          617 t
ORIGIN      
        1 cgagatgcac gaccgcagac tgcacacaca gtcgggcaag cacgccaggc aggttttgtt
       61 agcgactctt tggccgcccg cccggccctg acattctgga gcatctcatg agcctgaaag
      121 cccgcatcaa cgatgatatg aaagccgcca tgaaggcgcg cgaggccgag cgtctggcca
      181 ccatccgcct gctgctggcg gccatgaagc aaaaagaagt ggacgagcgc atcgagctgg
      241 acgacgccgc catcattgcc gtggtggaca aaatgctcaa gcagcgccgg gattcggtga
      301 cgcaatacga agccgccaac cgccaggatc tggccgatcg cgagcgcttt gaaatgaccg
      361 tgctggccga ctacctgccg cagccgctga gcgaagccga aatcgacgcg ctgatcgaag
      421 ccgccgtggc cagcactggc gccgccggcc tgccggacat gggcaaggtg atgggcgcag
      481 tcaaaggcca gatggccggc cgcgccgaca tggcggcggt gtctgcccgc atcaaggcgc
      541 ggctgacgcg ctgacgccag ccagggcggc gcgatgattc cgcaggattt cattgaccag
      601 ttgctgaccc gcgttgacat tgttgacgtg gtggagcgct acgtcccgct gaaaaaagcc
      661 ggccagaact acatggcctg ctgcccgttc cacaaagaaa aaagcccgtc gttctcggtg
      721 agccccagca agcagtttta ccactgcttt ggctgcgggg cgcacggcaa cgccatccgc
      781 tttgtgatgg agcacgccgg gctgggcttt atcgacgcgg tgaacgagct ggccggctcg
      841 gtgggcatgt ccgtgccgca agcgccggcc tcgccgcagc agcaacaggc ggcgcgccag
      901 cgccccagcc tgctggagtg catgggccag gcggcgcgct tttacaagca gtcgctcaag
      961 cgctcgcccg aggcgattgc ctacctgaaa aaacgcgggc tgaccggcga aatcgccgcg
     1021 cgctttgccc tgggctatgc gccggacggc tggacgccgc tgcaacaggc gtttgacgac
     1081 tatctggacg acaatctggt ggagtgcggc ctggtgctgc gccacgacga tggccgccgc
     1141 tacgaccgct ttcgtcatcg catcatgttt ccgatccgct ccagccgtgg cgacatcatc
     1201 ggctttggcg ggcgggtgct ggatcagggc gagccaaaat acctgaactc gccggaaacc
     1261 ccgctgtttg aaaagggccg cgagctgtac ggcctgtacg aagcgcgcca gggcatccgc
     1321 gacgctggcc gcgtgctggt ggtggaaggc tatatggacg tggtggcgct ggcgcaatac
     1381 ggcattgact acgccgtggc cgcgctgggc accgccacca cgcccaccca tgtgcaaaaa
     1441 cttctgcgcc aggctgacca tgtgttctat tgttttgatg gggacgccgc tggccagcgc
     1501 gccgcgtggc gcgcgctgga aaacagcctg gaagcgctgg tggatggcaa ggcgctgcac
     1561 tttttgtttt tgcccagcga gcacgacccg gacagctatg tgcgcgagtt tggccgcgac
     1621 gcatttgaac aagccttaac ccgcgacagc ctgccgctgt ccgcctacct ggtaaacgag
     1681 ctgacccggc gggtggatat gcaatcgcag gaaggccgcg ccgagctggt gaagctggcc
     1741 acgcccttgc tggccaaggt gggcgccccc actttaggcg tgatgctgcg ccggcgggtg
     1801 gctgagctgg cgcagatcga cctgaacgag ctggaccgcc tgaccgggcg cacgccgcgc
     1861 tttggcaaaa aaacccatcc gcgcggcggc gcgcgcggcc cccggcatga tccctccttg
     1921 gtgcgccggc tgattcgctg gctggcgctg aacccggtgc tggccgcgca ggtgcagctg
     1981 gacatcgatc cagccgaatg cgggccggaa ctgcaagcgc tgctggcgct ggcgctgttg
     2041 attcagtccg aagccacgcc gcccagcccg gcgcagctgg aagaacgcct gcgcgacacc
     2101 gaactgggcg aacgcttgcg ccccctgctg gtccaagcct ggcaggacgg cgacctggcc
     2161 gatgccttgc cgcaagaggt cagcgacgct tcacgacggc tggcggacaa tatccgtcac
     2221 gcccaattgg agcggctgac cgaaaaagaa cgtcatcagg gcctgacgga tgaagaaaaa
     2281 cgtcgcctgc ttgaactgct caggatgtca gccggcccaa gtcggtcttg atttgtgtta
     2341 taattttctg atttttcagc tcgatttttc caggacgcaa taatggcggc aactcccgaa
     2401 aacgataagg atgttgaaat caaagcgcaa acgctcgcgg acaacgccga cgagcgccgc
     2461 aagcggctaa aacaactgat cgtactgggc aaggaacgcg gctacctgac ctacgccgag
     2521 atcaacgacc acctgcccga agacgtgctc gacgccgagc agatcgaaag catcatcggc
     2581 atgatcaccg atatgggcat tcaggtctgc gacgaagccc ccgatgccga acagctgctg
     2641 atgtccgacg ccaccccggc ggtggcggac gaagacgccg tggaagaagc agaagccgcg
     2701 ctttccaccg tggattccga attcggccgc accactgacc cggtgcgcat gtatatgcgt
     2761 gaaatgggga cggtggagct tctgacccgt gaaggcgaaa tcgaaatcgc caagcgcatc
     2821 gaagacggcc tcaagcacat gattcaggcg atttccgcct gccccggcac cgtggccgaa
     2881 gtgctggcgc tgatcgaccg catcgaagcg ggcgagatgc gcgtggacga actcatcgac
     2941 ggcttcatcg acccggatgc cccggaagaa gaaaccgccg aattcgccga gcccagcgac
     3001 gaagaactgc tcgccgatga agaagacgac gaggaagaag acgaagacgg cgaagccgcc
     3061 gctgcggtca acaacgccaa tctggaagaa ctcaaggcgc aaacccttga gcactttggc
     3121 gtggtcaaag ccctgtacgt caagatgctc aaggcgctgg aaaagagcgg cagccagagc
     3181 aaggcctatc ttgagcagca agaagccatc accgccgaat tcatgaaggt gcgcttctcg
     3241 gtcaagcaag tggaagccct gtgcgaaagc ctgcgcagca gcgtggatga aatccgccgc
     3301 ttcgaacgcg aaatcatgga catctgcgag cagaaggcca agatgccgca cgcccgcttt
     3361 atcgaaacct tccccggcag cgaaaacgat ccggcctggg tggagcgcga aatcgccgtg
     3421 ggcaagccct acagcaatgc actcgagcgc ttcaagtacg ccatcatcga aaagcagagc
     3481 aagctgggcg agttgcagca aaaggcgatg attccgatca aagagctcaa ggacatcaat
     3541 aaacagatgg ccctgggcga atccaaggcg cgcatggcca aacgcgagat gatcgaggcc
     3601 aacctgcgtc tggtgatctc gattgccaag aagtacacca accgtggctt gcagttcctc
     3661 gacctgatcc aggaaggcaa tatcggcctg atgaaggcgg tggacaagtt cgaataccgc
     3721 cgtggctaca agttctcgac ttacgccacc tggtggattc gccaggccat cacccgctcg
     3781 atcgccgacc aggcgcgcac catccgcatt ccggtgcaca tgatcgaaac catcaaca
//
LOCUS       DAOPYX010000285         3803 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_31874_length_3803_cov_6.028015, whole genome shotgun sequence.
ACCESSION   DAOPYX010000285 DAOPYX010000000
VERSION     DAOPYX010000285.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 3803)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3803)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3803
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31874_length_3803_cov_6.028015"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            352..447
                     /locus_tag="PKN55_12190"
     CDS             352..447
                     /locus_tag="PKN55_12190"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0043462 - regulation of ATP-dependent
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit F"
                     /protein_id="HNX72051.1"
                     /translation="MDALTVCAGLLALGLAVWLIHALLNAEDIGC"
     gene            441..2132
                     /gene="kdpA"
                     /locus_tag="PKN55_12195"
     CDS             441..2132
                     /gene="kdpA"
                     /locus_tag="PKN55_12195"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018749689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HNX72052.1"
                     /translation="MLTAFFWLLALFMAVLTLSVRPMGAWLAPLASGQAPARLAGFDR
                     ALLRLMGVRDTAMDWREYARAVLLFSLLGALLLYVLQRVQGWLPLNPGQRGAVTADSA
                     FNTAVSFVSNTNWQGYAGEASMSHLTQMLGLTVQNFLSAATGIAVACVLMRGFARRET
                     RNLGNFWADLTRISVYLLAPLSLLLALALASQGVVQSWAATLPVATLAGGEQTLTLGP
                     VASQEAIKLLGTNGGGFFNANSAHPFENPNELSNFLQMLAIFLIPAGLCHAFGRVVGD
                     RRQGWALYGAMALLFVLAASALALAEAQGNPLLNALSADASVGSLEGKELRFGAAGSS
                     LFAAVTTAASCGAVNAMHDSLTPLGGMLAMWLMQLGEVVFGGVGSGLYGMLVFAVLAV
                     FVAGLMVGRTPEYLGKKIEAFEMKMVALVILLSPALVLLGSALAVVSQAGLAGLANGG
                     AHGFSEVLYAFSSAANNNGSAFAGLSANTPFYNGLLGAAMWLGRFGVIVPVLALAGAL
                     AAKKTLPPSAGAMPTHGGLFIGLLSGAVLMVGALTYLPALALGPMAEHLALWAGR"
     gene            2162..>3803
                     /gene="kdpB"
                     /locus_tag="PKN55_12200"
     CDS             2162..>3803
                     /gene="kdpB"
                     /locus_tag="PKN55_12200"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012697047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HNX72053.1"
                     /translation="MTQHSLPLFDAALLRPALLDACKKLSPRAQLGNPVMFVVYLGSL
                     LCSALWVQGLLGHGDAPTWLTGALALWLWFTVLFANFAEALAEGRSKAQAASLKGLKK
                     AAWAKKLGAPHRYGAPFATVEAGELRKGDVFIAQAGDIVPCDGEVLEGIAAIDESAIT
                     GESAPVIRESGGDFSSVTGGTRVLSDWVVARVTADPGETFIDRMIAMVEGARRGKTPN
                     EVALTILLLALTLVFLLVTVTLLPFSHYSAQAAGAGEPTSLAVLIALLVCLIPTTIGG
                     LLSAIGVAGMSRLMAANVIATSGRAVEAAGDVDVLLLDKTGTITYGNRQASALWPAPG
                     VSQATLAQAAWLASLADDTPEGKSIVQLTQPLLPPAANPLDAALRQGERPEAVSFAPF
                     TAQSRMSGVNLPERAIRKGAADAVRQWLAANGGELPAALQQQVDQVASRGGTPLVVAE
                     PQRALGVIELKDVVKPGIRERFAQLRAMGIRTVMLTGDNRLTAAAIAAEAGVDDFLAE
                     ATPEAKLAMIRDCQRQGLLVAMTGDGTNDAPALAQADVA"
BASE COUNT          545 a         1214 c         1345 g          699 t
ORIGIN      
        1 gcccatttgc gccgctggct gagcggcaaa gagcagtttc tgcgcgcctg agcgcagccc
       61 atcctgatgt gtggcgacag cgcagcggcc gccaacccct tttgtgcggc ggcaatcccg
      121 ccgccgcaca agccgccaac ggcctgaccc tgaccgtttt tacgccattt ttacacccgc
      181 cctcccagtc ttgatcctgt ctttacgccg tgccgcctag agtggcggcg tcgatttccg
      241 acaggagaat ttcatgaacg atctgatctg gctgaccgtg ttgctggcgc tgtacgccgc
      301 cacccgcggt ctggtgactg tctgcgcggc gcgtcagcgc aagggggcgt gatggatgcg
      361 ctgacggtgt gcgccggcct gctggcgctg gggctggccg tatggctgat tcatgccctg
      421 ctcaatgcgg aggacatcgg atgttgaccg catttttttg gttgctggcg ctgttcatgg
      481 cggtgctgac gctcagcgtg cggccgatgg gcgcctggct ggcgccgctg gccagcggac
      541 aggcgccggc gcggctggcg gggtttgacc gcgccttgct gcgcctgatg ggcgtgcgcg
      601 acacggcgat ggactggcgc gaatacgccc gcgccgtgct gctgttcagc ctgctgggcg
      661 cgctattgct gtatgtgctg caacgcgtgc agggctggct gccgctcaac cccggccaac
      721 gcggcgcggt gacggcggat tcggcgttca acaccgcggt gagctttgtc agcaacacca
      781 actggcaagg ctacgccggt gaggcgagca tgagccacct gacgcagatg ctcgggctga
      841 ccgtgcagaa ctttttgtct gccgccaccg gcattgccgt ggcctgcgtg ctgatgcgcg
      901 gctttgcccg gcgggaaacc cgtaacttgg gcaatttctg ggccgatctg acccgcatca
      961 gcgtatacct gctggcgcct ttgtcactgc tgctggcgct ggcgctggcc agtcagggcg
     1021 tggtgcaaag ctgggcggcc acgctgccgg tggccacgct ggccggcggc gagcaaacgc
     1081 tgaccctggg cccggtggcc tcgcaagaag ccatcaagct attgggcacc aatggcggcg
     1141 ggtttttcaa cgccaactcg gcccatccgt ttgaaaaccc caatgagctg agtaatttcc
     1201 tgcaaatgct ggcgattttt ttgattccag ccgggctatg ccatgccttt ggccgagtgg
     1261 tgggcgaccg ccgccagggc tgggcgctgt atggggcgat ggcgctgctg tttgtgctgg
     1321 cggccagtgc gctggcgctg gccgaggcgc agggcaaccc gctgctgaac gcgctgagcg
     1381 ccgacgccag cgtcggcagc ctggaaggca aggaactgcg ctttggcgca gcgggctcca
     1441 gcctgtttgc cgccgtgacc acggcggcca gttgcggcgc ggtgaacgcc atgcacgatt
     1501 cgctcacccc gctgggtggc atgctggcca tgtggctgat gcagctgggc gaagtggtgt
     1561 ttggcggggt gggctctggg ctgtacggca tgctggtgtt tgccgtgctg gcggtgtttg
     1621 tcgccgggct gatggtgggg cgcacgccgg aatacctggg caaaaaaatc gaagccttcg
     1681 agatgaagat ggtggcgctg gtgattttgc tgtcgccggc gctggtgctg ctgggttcgg
     1741 cgctggcggt ggtcagccag gccgggctgg cggggctggc caatggcggc gcgcatgggt
     1801 tttctgaagt gctgtatgcg ttttcttccg cagccaataa caacggcagc gcctttgccg
     1861 ggctgtcggc caatacgccg ttttataacg gcctgctggg ggcggccatg tggctggggc
     1921 gctttggcgt gatcgtgccg gtgctggcgc tggccggcgc actggccgcc aagaaaaccc
     1981 tgccgccgtc ggccggggcc atgcccaccc atggcgggct gttcattggc ctgttaagcg
     2041 gcgctgtgct gatggtgggc gcgctcacct atttgccggc gctggcgctg gggccgatgg
     2101 cggaacacct ggcgctgtgg gctggccgct gagcgccgcg cgcacaatta aggaaatcat
     2161 catgacgcaa cattctctgc cgctgttcga cgccgcgctg ctgcgcccgg cgctgctgga
     2221 cgcctgcaag aaactctcgc cccgcgccca actgggcaac ccggtgatgt ttgtggttta
     2281 cctgggcagc ctgctgtgct cggcgctgtg ggtgcaaggg ctgctgggcc acggcgacgc
     2341 ccccacctgg ctcactggcg cgctggcgct gtggctgtgg ttcaccgtgc tgttcgccaa
     2401 ctttgccgaa gccctggccg aggggcgcag caaggcgcaa gcggccagcc tgaagggcct
     2461 gaaaaaagcc gcctgggcca aaaaactggg cgcgccacac cgctacggcg cgccgtttgc
     2521 caccgtcgag gccggtgagc tgcgcaaagg cgatgtgttc atcgcccagg cgggcgacat
     2581 cgtgccctgc gacggcgaag tgctcgaagg catcgccgcc atcgacgaat ccgccatcac
     2641 cggcgaatcc gccccggtca tccgcgaatc cggcggcgat ttcagctcgg tcaccggcgg
     2701 cacccgcgtg ctgtcggact gggtggtggc gcgggtgacc gccgatccgg gcgaaacctt
     2761 tatcgaccgc atgatcgcca tggtggaagg cgcgcggcgc ggcaaaaccc ccaatgaagt
     2821 cgcgctgacc atcctgctgc tggcgctgac cctggtgttt ttgctggtga ccgtcaccct
     2881 gctgccgttt tcgcactata gcgcccaggc tgcgggcgcg ggcgaaccga cctcgctggc
     2941 agtgctgatc gccctgctgg tgtgcctgat tcccaccacc atcggcggtc tgctgtcggc
     3001 gattggcgtg gcgggcatga gccggctgat ggccgccaat gtgattgcca cctccggacg
     3061 ggcagtagaa gccgctggcg atgtggacgt actgttgctg gataaaaccg gcaccatcac
     3121 ctatggcaac cgccaggcca gcgcgctgtg gccggcgccc ggcgtgtcgc aggcgacact
     3181 ggcgcaggcg gcatggctgg cctcgctggc cgacgacacc ccggagggca agagcattgt
     3241 tcagctcacc cagccgctgt tgccgcccgc ggccaatccg ctggatgccg cgctgcgtca
     3301 gggcgagcgg cccgaggcgg tcagctttgc gccgtttacc gcccagtcgc gcatgtcggg
     3361 cgtgaatttg cccgagcgcg ccatccgcaa gggcgccgcc gatgcggtgc gtcagtggct
     3421 ggccgccaat ggcggcgagc tgcccgccgc gctgcaacag caggtggacc aggtggccag
     3481 ccgtggcggc acgccgctgg tggtggccga gccgcagcgc gcgctggggg tgatcgagct
     3541 gaaagacgtg gtcaagcccg gcatccgcga gcgttttgcc cagttgcgcg ccatgggcat
     3601 ccgcacggtg atgctgaccg gcgacaaccg gctgaccgcc gccgcgattg ccgccgaagc
     3661 cggcgtggac gactttctgg ccgaagccac ccccgaagcc aagctggcga tgattcgtga
     3721 ctgccagcgc cagggcctct tggtggcaat gaccggcgac ggcaccaacg acgccccggc
     3781 gctggcccag gccgatgtgg cgg
//
LOCUS       DAOPYX010000286         3781 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_32139_length_3781_cov_13.508857, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000286 DAOPYX010000000
VERSION     DAOPYX010000286.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 3781)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3781)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3781
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32139_length_3781_cov_13.508857"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..481)
                     /locus_tag="PKN55_12205"
     CDS             complement(<1..481)
                     /locus_tag="PKN55_12205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNX72054.1"
                     /translation="MKRLNLSEWAIRHASLVRYLMIILLIGGVWAYGKLGQKEDPEFT
                     FKAMLVRVVWPGASTDDMQLQVTDRVEKKLMEHPDLDFVKSYTKPGETVLIVNLKESV
                     APKDVANAWYQVRKKLSDIRQQLPSDIRGPFFNDEFGDTFGNIYAFTGDGLGYEDLRR
                     "
     gene            complement(478..1572)
                     /locus_tag="PKN55_12210"
     CDS             complement(478..1572)
                     /locus_tag="PKN55_12210"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNX72055.1"
                     /translation="MTIRHFAALCLVGALAACSKPEAQPEAPRPVRSVTLGASNGQTA
                     AFHAGDIRARHEARLAFRVGGKVVARLVDVGATVKPGQALARLEDNDTSLDLASRRAK
                     LAAAEADLKQNEADIKRYRELLAQHFISQAEFDRREAALAAARGNQQAALAAAQLGAN
                     QQGYTTLTAERAGVVTSIDAEVGQVVSAGQVVARVAESSEREAVIAVAESQLAEVRAA
                     RGFVVQVGQLPGQYQGVLRELAPDADSVSRTYTARITLKDADDSVRLGMSARVYPQGE
                     AAAPLSVPLSAVLDENNRHYLWLLDGKAPQVKRVEVKLAKVSSDTAWIASGVRAGQEV
                     VTAGVHLLRDGQTVRRLTHDPAQPVGTQAK"
     gene            complement(1943..2587)
                     /locus_tag="PKN55_12215"
     CDS             complement(1943..2587)
                     /locus_tag="PKN55_12215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018746822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNX72056.1"
                     /translation="MTATPPASAPATRWSRRKDVRPGEILEAALKLFVEKGFRATKME
                     DIAREAGVTKGTPYLYYENKEEIFKAVIREGMLTKFDELEGLSGQDEASAETRLRQVI
                     YRWWESIGNTRLAGLSKLMIAEAANFPELASFYHAEVIVRCRNMVRHVLEEGVERGEF
                     HILNMDHAVEIVIAPLLLAMVWKHSFHNFESAKFDMPAHLDNVLDILINGLKTR"
     gene            2747..3631
                     /gene="argB"
                     /locus_tag="PKN55_12220"
     CDS             2747..3631
                     /gene="argB"
                     /locus_tag="PKN55_12220"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014085818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HNX72057.1"
                     /translation="MTLTPHLAQDKAAILSEALPYIRKFAGKTIVIKYGGNAMTDDAL
                     KEGFAKDVVLLKLVGLNPVVVHGGGPQINQLLARIGKQGEFVQGMRVTDSETMDVVEM
                     VLGGLVNKEIVSLINQHGGKAVGVTGKDGSFIRARKMFLKADDGEESIDIGQVGEIES
                     IDPSLIALMDTRDFIPVIAPIGVGHDGSAYNINADLVAGKLAETLGAEKLILMTNTEG
                     VLDKNGQLLTGLTASQVDGLFADGTIHGGMLPKISSALDAAKNGVQSVHIIDGRVKHA
                     LLLEILTDAGVGTMIRAN"
BASE COUNT          700 a         1223 c         1138 g          720 t
ORIGIN      
        1 agcggcgcag gtcttcgtag cccagcccgt cgccggtaaa ggcgtagata ttgccaaaag
       61 tgtcgccaaa ttcgtcgttg aaaaacggcc cgcgaatgtc gctgggcagt tgctggcgaa
      121 tatcagacag ctttttgcgc acctgatacc aggcattggc cacatctttg ggggccacgc
      181 tttctttcag gttgacgatc agcaccgtct cgcccggctt ggtgtagctt ttgacgaagt
      241 ccagatcggg gtgctccatc agcttttttt ccacccggtc ggtgacttgc agctgcatat
      301 cgtcggtgct ggcccccggc cacaccacgc gcaccagcat ggccttgaag gtgaattccg
      361 ggtcttcttt ctgccccagc ttgccatacg cccacacccc gccaatcagc aggatgatca
      421 tcaaatagcg caccagcgag gcgtggcgaa tcgcccattc cgagagattg agtcgtttca
      481 tttcgcctgc gtccccaccg gctgcgccgg atcatgcgtg agtcggcgca cggtctggcc
      541 gtcgcgcagc aggtgcacgc cagcggtgac cacttcttgc ccggcgcgca cgccgctggc
      601 aatccaggcg gtgtcgctgc tgaccttggc cagtttgact tccacccgct tgacctgcgg
      661 cgccttgccg tccagcagcc acagataatg gcgattgttt tcgtccagca cggcgctcag
      721 cggcacgctc agcggcgcgg cggcctcgcc ctgcgggtag acgcgcgcgc tcatgcccag
      781 gcgcacgctg tcgtcggcgt ccttgagggt gatgcgcgcg gtgtaggtgc ggctgacgct
      841 gtcggcgtcc ggcgccagct cgcgcagcac gccctgatac tggccgggca gttggcccac
      901 ttgcacgaca aaaccgcgcg cggcgcgcac ctcggccagt tgcgattcgg ccacggcaat
      961 caccgcctcg cgctcgctgg attccgccac ccgcgccacc acttggccgg cgctgaccac
     1021 ctggccaact tcggcgtcga tgctggtcac cactccggcg cgctcggcgg tcagcgtggt
     1081 gtagccctgc tgatttgcac ccaactgggc ggcggccagc gctgcctgct gattgccgcg
     1141 cgcggcggcc agcgccgctt cgcggcggtc aaactcggcc tggctgataa agtgctgggc
     1201 cagcagctcg cggtagcgct tgatgtcggc ttcgttctgc ttcaggtcgg cctcggcagc
     1261 ggccagcttg gcgcggcggc tggccagatc caggctggtg tcgttgtctt ccagccgcgc
     1321 cagcgcctgg cctggtttga ccgtggcgcc aacgtccacc agccgggcca ccaccttgcc
     1381 gccgacgcga aacgccagcc gcgcctcgtg gcgggcgcgg atgtcgccag cgtgaaacgc
     1441 ggcggtctgc ccgttgctcg cccctaaagt gacgctgcgc accggccgtg gcgcttctgg
     1501 ctgcgcctcg ggtttgctgc acgccgccag cgcgcccacc agacacagcg cggcaaaatg
     1561 acgaatggtc atggttgtgt ttccttcgtg tagtcgatca ggcgtttctg cgtcgcctgc
     1621 gtgtgcaatc cagccgctct tgccgctgct ggcgcagtag caatgtagta attgttaact
     1681 taatgggtga acaaaacccg ccgggcggca aggccgcacc ccggcggggt gatgaaaacc
     1741 caaacagcgc aacgcttaga gccgctaaca aaaccccctg gcgttgttgt gtgaccttgt
     1801 cgtactactt gtactgtctg cggtcacacg cctagccagg accgcttcgc tgggttttgt
     1861 tagcggctct taacacaaaa cccggcacag caaccctggc taggcgcgcg ggcgcacacc
     1921 aggagcgttt tgtcagccgc gcttagcggg ttttcaggcc attgatcaag atatccagca
     1981 cattatccaa gtgcgccggc atgtcaaact tggccgattc aaaattatga aacgaatgct
     2041 tccacaccat ggccagcagc agcggggcga tgacaatctc caccgcgtga tccatgttga
     2101 ggatgtgaaa ctcgccgcgc tcgacgcctt cttccagcac atggcgcacc atattgcgac
     2161 agcgcacaat cacttcggcg tggtaaaagc tggccagctc aggaaaattg gccgcctcgg
     2221 cgatcatcag cttggacaag cccgccaggc gggtattgcc gatggactcc caccagcggt
     2281 aaatcacctg gcgcagccgg gtttccgcgc tggcttcatc ctggccggac aagccttcca
     2341 gctcgtcgaa cttggtcagc atgccttcgc ggatcaccgc cttgaagatt tcttccttgt
     2401 tttcgtagta cagatacggc gtgcccttgg tcacccccgc ctcgcgggcg atgtcttcca
     2461 tcttggtggc gcgaaagcct ttttccacaa acagtttcag cgcggcttcc aggatttcgc
     2521 cagggcgaac gtccttgcgg cgagaccagc gggtggcagg ggcggaagcg ggcggggtgg
     2581 ccgtcatctg ggataccata acaagcgaat gatcagactt actctatagt aaggcgatgc
     2641 agtcggcaag gtataggtaa atgtgaattc agataaaccc ggcgcggcgt tgcgtttaca
     2701 atacggcttt ccgaccccgt cgttatttgt ctcacaggaa ccgaccatga cgctgacccc
     2761 gcacctggcc caggacaagg ccgccatcct ctctgaagcg ctgccttaca tccgcaaatt
     2821 tgccggcaag accatcgtga tcaagtacgg cggcaacgcc atgaccgacg acgccctgaa
     2881 agaaggcttc gccaaagacg tggtgctgct caagctggtg ggcctgaacc cggtcgtggt
     2941 gcacggcggc ggcccgcaaa tcaaccagtt gctggcgcgc atcggcaagc agggcgaatt
     3001 tgtccagggc atgcgcgtga ccgacagcga aaccatggac gtggtggaaa tggtgctggg
     3061 cggcctggtc aacaaagaaa tcgtcagcct gatcaaccag cacggcggta aggccgtggg
     3121 cgtgaccggc aaggacggca gctttatccg cgcgcgcaag atgttcctca aggccgacga
     3181 tggcgaagaa tccatcgaca tcggtcaggt gggcgaaatc gaatccatcg accccagcct
     3241 gattgcgctg atggacaccc gcgactttat cccggtgatc gccccgattg gtgtgggcca
     3301 tgacggctcg gcctacaata tcaacgccga cctggtggcc ggcaagctgg cggaaaccct
     3361 gggcgccgaa aaactgatcc tgatgaccaa caccgaaggc gtgctggaca aaaacggcca
     3421 gctgctcacc ggcctgaccg ccagccaggt ggacggcctg tttgccgacg gcaccatcca
     3481 cggcggcatg ctgcccaaga tcagctcggc gctggacgcc gccaaaaacg gcgtgcaatc
     3541 ggtgcacatc atcgacggcc gggtgaaaca cgccctgctg ctggaaatcc tcaccgatgc
     3601 cggcgtgggc accatgatcc gcgccaatta agcgcggctc accaagccct ccttgtcgtg
     3661 tctgcggccg cacacctcgc caggatcgct gcgcagggtt tgctggaccg ctcttgaccc
     3721 ggcggattca agcgcaaaac ggcgagccat cgggctcgcc gttttgtttg ccacagacac
     3781 a
//
LOCUS       DAOPYX010000287         3780 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_32160_length_3780_cov_9.760805, whole genome shotgun sequence.
ACCESSION   DAOPYX010000287 DAOPYX010000000
VERSION     DAOPYX010000287.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 3780)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3780)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3780
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32160_length_3780_cov_9.760805"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..185)
                     /locus_tag="PKN55_12225"
     CDS             complement(<1..185)
                     /locus_tag="PKN55_12225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX72058.1"
                     /translation="MKNSSQNQNIENFCSSAPRNIVRHAPSPATRRQIALPLATAQTS
                     RTAHRLPRPPDAPIKHM"
     gene            245..>1578
                     /locus_tag="PKN55_12230"
     CDS             245..>1578
                     /locus_tag="PKN55_12230"
                     /inference="COORDINATES: protein motif:HMM:NF024924.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HNX72059.1"
                     /translation="MKHHLDWSSYETAGQGDAYAGIPATGGDFARAVAVCIGDRLCQR
                     TPKGVMCPSFRVSGEIAHSPGARVLALKAALNGELGAAPFADARLAEAMELCVGCKGC
                     KRECANQVDMAAIKIEWQAQRNAALGVSRRDQLFAGLPRLLHRRPWLGALIGWRNRSP
                     LLAQLGERWLGVAASRQLPQPAARPYLPPAIAPVLGERSVFLLVDTFAGLFQPHIAMA
                     AQAVLHAAGYQVHVLRPLADDAEPARPLCCGRTYLSLGQVDAAREEARRLHAALAPAL
                     ASGAPIVGLEPSCILSLRDDHLKLGLGEDARQVARQVFLFEEFIAREHDRKRLRWQLK
                     PWTQPPMLVHGHCHQKAVGAMKSLRKVLRLIPGCQFEFIDASCCGMAGSFGLDAEHAD
                     ISRAMAELDLLPALRAAPDAPVLANGFSCRHQIEEGCGRAPRHIAQWLAEALA"
     assembly_gap    1579..1678
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1719..3386
                     /locus_tag="PKN55_12235"
     CDS             1719..3386
                     /locus_tag="PKN55_12235"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HNX72060.1"
                     /translation="MSSTDVNLSAAEARNYHLRSKHHMQRYAEGPGQLDWDAQPAPFR
                     QFAGAPTLKLPFCAADQDRPFAELTQPHPAPLPVTLANVAALLQLSLGLSAWKSWQGS
                     RWALRNNPSSGNLHPVEGYLLARGVPGLADGVHHYDPLAHALEGRALTAPSAAPCWLA
                     VGLSSVMWREAWKYGERAFRYCQLDIGHAMAAFGYAAASLGWTVAAQPLASGELAALL
                     GLDRESDYPSGRYAYTEDEEPEVLLWLGAPGVTPPPGAAALADWLGAAVWQGQPSVID
                     PQPGYRWPVIDRVAVATRVHEAPPAGWTPTPGQLSPTPSPHASPASAQTLIQQRRSGQ
                     RYQPRHAMPAADFYRLLQALLPHEGAPWHVPAMAPAVHLLLCVLRVDGLSPGLYLLPR
                     QPGRVTQWPELALHFPERAPVSALHPDCPRGLGLLRLRTLELPEAQRFMRALCCHQDI
                     ASTGAFTVAMLGEFDATLDAHGGHGYRLLLREAGQIGQALYLEAEAAGLRGTGIGCFF
                     DNTLHGALGLNDARLQTLYHFTVGLPVVDARVETTPPYPDADLTERP"
     gene            3386..>3780
                     /locus_tag="PKN55_12240"
     CDS             3386..>3780
                     /locus_tag="PKN55_12240"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HNX72061.1"
                     /translation="MTPELREFLLEHGFPLDDPDAPGANGLTPLMRAAKLGRIELIDP
                     LLAHGARVDVLNQDGCNALWLACYHGDPALVRKLVAVGAPIDQQNGNGASCLMYVSSN
                     GKAELVEVLLELGADPTLKNFDDWTALDLA"
BASE COUNT          515 a         1207 c         1316 g          642 t
ORIGIN      
        1 tgcatgtgtt ttattggtgc atcgggtggg cgcggcaagc ggtgggccgt gcgcgacgtc
       61 tgtgccgtcg ccagaggcag ggcgatctgg cggcgcgtcg ccgggctggg cgcgtgtcgg
      121 acaatgttgc gcggggcgga tgaacaaaaa ttctcaatat tttgattttg tgatgaattt
      181 ttcatggcct tgtccttgct tgcagacacg gtaaagcgcg acgctgtgta aacaagaggc
      241 cgtgatgaaa caccatctgg attggtccag ctacgaaacc gccggccagg gcgacgccta
      301 tgccggcatc cccgccactg gcggcgattt tgcccgcgcc gtggcggtgt gcattggcga
      361 ccggctgtgc cagcgcacac ccaagggggt gatgtgcccc agctttcgcg tcagcggcga
      421 gatcgcccac tcgccggggg cgcgggtgct ggcgctgaag gcggcgctga atggcgagct
      481 gggcgccgcg ccgtttgccg acgcgcggct ggccgaggcc atggagctgt gcgtgggttg
      541 caagggctgc aagcgcgaat gcgccaatca ggtggacatg gcggcgatca aaatcgaatg
      601 gcaggcgcag cgcaacgccg ccctgggcgt cagccgccgc gaccagctgt ttgccggcct
      661 gccgcgcctc ttgcatcggc ggccgtggct gggcgcgctg attggctggc gcaaccgcag
      721 cccgctgctg gcgcaactgg gcgagcgctg gctgggggtg gccgcctcgc gccagctgcc
      781 gcagcctgcg gcgcggccgt atttgccgcc agcgattgcg cccgtcctgg gcgagcgcag
      841 cgtgtttttg ttggtggaca cctttgccgg gctgtttcag ccgcacatcg ccatggcggc
      901 gcaggcggtg ctgcacgccg ccggctatca ggtgcatgtg ctgcgcccgc tggccgacga
      961 cgccgagccg gcgcggccgc tgtgctgtgg ccgcacctat ctgtccttgg gccaggtgga
     1021 cgccgcgcgc gaagaagccc gccggctgca cgccgcgctg gcgccggcgc tggccagcgg
     1081 cgcgccgatt gtcgggctgg agccgtcgtg catcctgtct ttgcgcgatg atcacctcaa
     1141 gctgggtctg ggcgaggacg cccgtcaggt ggcgcgccag gtgtttctgt ttgaagaatt
     1201 catcgcccgc gagcacgacc gcaagcggct gcgctggcag ctcaaaccct ggacgcagcc
     1261 gccgatgctg gtgcacggtc attgccacca gaaagccgtg ggggcgatga aatccctgcg
     1321 caaggtgctg cgtttgattc cgggctgcca gtttgaattt atcgacgcca gctgttgcgg
     1381 catggccggc agctttggcc tggacgccga gcacgccgat atttcgcgcg ccatggccga
     1441 actggatttg ctgccggcgc tgcgcgccgc gcccgacgcg ccggtgctgg ccaatggctt
     1501 ttcctgccgt catcagatcg aagaaggctg tggccgcgcg ccccggcata ttgcccagtg
     1561 gctggctgag gccttggcnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnncg
     1681 gcttgtttcc ctgtttttca tctggtctgg agcccgccat gagcagcact gacgtgaatt
     1741 tgtccgccgc cgaagcgcgc aactaccacc tgcgcagcaa acaccatatg cagcgctacg
     1801 ccgaaggccc cggccagctg gactgggacg cccagccggc gccgtttcgc cagtttgccg
     1861 gcgcgcccac cctcaagctg ccgttttgtg cggcggacca agaccgcccg tttgccgagc
     1921 tgacgcagcc gcacccggcg ccgctgccgg tgacgctggc caatgtcgcc gcgctcttgc
     1981 agctgagcct gggcttgtcg gcctggaaaa gctggcaggg cagccgctgg gcgctgcgca
     2041 acaatccctc gtccggcaat ctgcatccgg tggaaggcta tctattggcg cgcggcgtgc
     2101 ccggcctggc cgatggcgtg caccattacg acccgctggc gcacgcgctg gaaggccgcg
     2161 cactgaccgc gcccagcgcc gcgccgtgct ggctggcggt ggggctgagt tcggtgatgt
     2221 ggcgcgaggc gtggaaatac ggcgagcggg cgtttcggta ttgtcagctg gatattggcc
     2281 acgccatggc ggcgtttggc tacgccgccg ccagcctggg ttggacggtg gcggcgcagc
     2341 cgctggccag cggcgagctg gcggcgctgc tggggctgga tcgggagagc gactacccca
     2401 gcggccgcta cgcctacacc gaagacgaag aaccggaagt cctgctgtgg ctgggcgcgc
     2461 cgggggtgac gccgccgccg ggcgccgccg cgctggcgga ttggctgggc gcggcggtct
     2521 ggcagggcca gcccagcgtg atcgaccccc agcccggcta ccgctggcca gtgatcgacc
     2581 gcgtggcggt ggccacccgc gtccatgagg cgccgccagc aggctggacg cccacgccgg
     2641 gccagttgtc gcccacccca tcgccgcacg ccagcccggc cagcgcgcag acgttgatcc
     2701 agcaacggcg cagcggccag cgttatcagc cgcgccacgc catgccggcg gcggattttt
     2761 accggctgtt gcaggcgctg ctgccccacg agggcgcgcc ctggcatgtg ccggccatgg
     2821 ccccggcggt gcatttgctg ctgtgcgtgc tgcgggtgga cgggctcagc ccgggtttgt
     2881 atttgctgcc gcgccagccc ggccgggtga cgcagtggcc agagctggcg ctgcattttc
     2941 ctgagcgtgc gccggtcagc gcgctgcacc cggattgccc gcgcggcctg ggcctgctgc
     3001 ggctgcgcac gctggagttg cccgaggcgc agcgctttat gcgcgcgctg tgctgtcatc
     3061 aggacatcgc ctcgaccggc gcattcaccg tggccatgct cggcgaattc gacgccaccc
     3121 tggacgcaca cggcggccac ggctaccggc tgctgctgcg cgaagccggg cagattggcc
     3181 aggcgctcta tctggaggcc gaagccgccg gcctgcgcgg cactggcatt ggctgttttt
     3241 ttgacaacac cttgcacggc gcgctgggcc tgaacgacgc gcgtctgcaa accttgtatc
     3301 actttaccgt cggcctgccg gtggtggacg cgcgggtgga aaccacgccg ccctacccgg
     3361 acgctgacct caccgaaagg ccgtgatgac ccccgaattg cgtgaatttt tattggagca
     3421 tggttttccg ctggacgacc cggacgcccc cggcgccaat ggcctgacgc cgttgatgcg
     3481 cgccgccaag ctggggcgca tcgagctgat cgaccccttg ctggcgcatg gcgcgcgggt
     3541 ggacgtgctc aatcaggatg gctgcaatgc gctgtggctg gcctgctacc acggcgaccc
     3601 ggcgctggtg cgcaagctgg tggcggtggg cgcgccgatt gaccagcaaa acggcaacgg
     3661 cgccagctgt ttgatgtatg tgtcgtccaa tggcaaggcc gagctggtgg aggtgttgct
     3721 ggaactgggc gcggacccga cgctgaagaa ttttgacgac tggaccgcgc tggatctggc
//
LOCUS       DAOPYX010000288         3778 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_32189_length_3778_cov_7.956487, whole genome shotgun sequence.
ACCESSION   DAOPYX010000288 DAOPYX010000000
VERSION     DAOPYX010000288.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 3778)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3778)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3778
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32189_length_3778_cov_7.956487"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1329
                     /locus_tag="PKN55_12245"
     CDS             <1..1329
                     /locus_tag="PKN55_12245"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014087995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HNX72062.1"
                     /translation="GRDAAMIFQEPMTALNPLYSVGNQICETLTLHLGLSPRQARAEA
                     LALLARTGIPDPASKIDSYPFQLSGGQRQRAMIAMALACRPRLLIADEPTTALDVTVQ
                     AQILDLLADVQREFGMAVWLITHDLNLVRRFADRVAVMRAGQIVESGPVAQVFAQPAH
                     DYTRQLLASRPGRLVDAGDPKAAEVARAEQLSVSYRQRAGWLRSRDIPILRDVSLRLR
                     AGHTLGIVGESGSGKTTLGLALLKLVNAQTRGRVVVAGQALDGLPARALRAFRRQAQV
                     VFQDPFSSLSPRLTVGEIVGEGLALHEPQLSASERQARVAAMLAEVGLSADCLPRYPH
                     EFSGGQRQRIAIARALVLRPQLLLLDEPTSALDASIQQQVLTLLRDAQQRYGVAYLFI
                     SHDLAVIRAMAHDVLVLRHGQVVESGPAEQLFTAPQAEYTRALLAAARVG"
     gene            complement(1351..1872)
                     /locus_tag="PKN55_12250"
     CDS             complement(1351..1872)
                     /locus_tag="PKN55_12250"
                     /inference="COORDINATES: protein motif:HMM:NF013231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HNX72063.1"
                     /translation="MSQPNDPVATILQQWRRERPDLDVTPMGPVGRLRRSAVQVQKRL
                     DTVFARHQLASWEFDLLATLRRSGEPYRLAPTVLFSSLMLTSGAMTRQLQQLEKRGLI
                     QRQINPADARSVLVQLSAEGLALIDSAVGEHVDNLHRLTSTLDATELAQLDSLLGKWM
                     AGLEGEGEKGRDM"
     gene            1958..2839
                     /locus_tag="PKN55_12255"
     CDS             1958..2839
                     /locus_tag="PKN55_12255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003824801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HNX72064.1"
                     /translation="MFPVMSASARDALLTACAPLIWGSTYWVTSQWLPPDRPLTAAVW
                     RVLPAGLLLLLWARQWPAAGQWRRVFGLAVLNIAVFQALLFVAAYRLPGGVAAILGAT
                     QPLLVLGWAWLLDGQRPAGAVTTAAVLGVAGMALLLNAPGAQWDTVGVLAAALGAASM
                     ACGSYLAGRWQGCQLPVLALTGWQLTLGGLCLAPLAWAWEPPLPALSLTQGLAYGYLS
                     LFGALLAYVLWFRGIVRLPAVAVSSLSLLSPLSAAVIGALALGQWLHGWALAGWLLTL
                     GSVLGVQRLSQRRRSRQ"
     gene            complement(2815..3765)
                     /gene="rarD"
                     /locus_tag="PKN55_12260"
     CDS             complement(2815..3765)
                     /gene="rarD"
                     /locus_tag="PKN55_12260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HNX72065.1"
                     /translation="MFFALSASQQTDCAIPIMTDSLQETRRGVLFAMAAFFIWGLFPL
                     YWKPLGQTPALQILCHRIVWSAVFVAGVLLWQRHWAWLAVSVRQPRLLGLFVASSLLL
                     SVNWLIYIWAVNENRVVEASLGYFINPLVNVLLGRVFLGERLSPPQALAVLLAALGVA
                     WLTLSLGALPWVALSLAASFGVYGLLRKQAPLPSLEGLALETFLLAPLAALALAWFHW
                     QGQGAFGQHGLTVDALLMGAGVVTAVPLLLFASGARRLKLATVGLIQYIGPTMQLLLG
                     VWLYHEPFGAARAVGFGLIWTALALYSGHGLLSYWRERRR"
BASE COUNT          608 a         1244 c         1304 g          622 t
ORIGIN      
        1 gggcgcgacg cggcgatgat ttttcaagag ccgatgaccg cgctgaaccc attgtacagc
       61 gtgggcaacc aaatctgcga aaccctcacc ctgcacctgg gcctgagccc acgccaggcg
      121 cgcgccgaag cgctggcgct gctggcgcgc accggcatcc ccgacccggc cagcaagatc
      181 gacagctatc cttttcagtt atccggcggc caacgccagc gggcgatgat cgccatggcg
      241 ctggcctgcc gcccgcgcct gttgatcgcc gacgaaccca ccaccgcgct ggacgtcacc
      301 gtgcaagcgc aaatcctgga tttgctggcc gatgtgcagc gcgaatttgg catggcggtg
      361 tggctgatta cccacgacct gaacctggtg cgccgctttg ccgaccgggt ggcggtgatg
      421 cgcgccgggc agattgtcga atccggcccg gtggcccaag tatttgccca acccgcgcac
      481 gactacaccc gccagctatt ggccagccgc cccggccggc tggtggacgc gggcgacccc
      541 aaggccgccg aagtggcccg cgccgagcaa ttatcggtca gctaccgcca acgcgccggc
      601 tggctgcgca gccgcgacat ccccatcctg cgcgatgtct ccctgcgcct gcgcgccggc
      661 cacacgctgg gcattgttgg cgaatccggc tcgggcaaaa ccacgctggg cctggccctg
      721 ctgaaactgg tgaatgcgca aacgcgcggg cgggtggtgg tggccggcca ggcgctggac
      781 ggcctgccgg cgcgcgcgct gcgcgcattt cgccgccagg cgcaagtggt gtttcaagac
      841 ccgttctcca gcctgtcgcc gcgcctgacc gtgggcgaaa tcgtcggcga aggcctggcc
      901 ctgcacgaac cccagctcag cgccagcgaa cgccaggcgc gggtggccgc catgctggcc
      961 gaagtcggcc tgtcggccga ctgcctgccg cgctacccgc atgaattttc tggcggccaa
     1021 cgccagcgca tcgccatcgc ccgcgccctg gtgctgcgcc cgcagctatt gctgctggac
     1081 gaacccacct cggcgctgga cgcctcgatc cagcaacaag tgctcaccct gctgcgcgac
     1141 gcccagcaac gctacggcgt ggcctatctg ttcatcagcc acgacctggc ggtgatccgc
     1201 gccatggccc acgacgtgct ggtgctgcgc cacggccaag tggtggaaag cggcccggcc
     1261 gaacaactgt tcaccgcccc gcaggcggag tacacccgcg cgctgttggc ggcggcgcgg
     1321 gtggggtgat ggaaaacggg tatacccctc ttacatatca cggccctttt ccccctcgcc
     1381 ctccagcccc gccatccact tccccaacaa gctatccagc tgtgccagtt ctgtcgcgtc
     1441 cagtgtgctg gtgagtcgat gcaggttgtc cacgtgctcg ccaaccgcgc tgtcgatcag
     1501 cgccaggcct tcggcgctca gttgcaccag cacgctgcgg gcgtcggcgg ggttgatctg
     1561 gcgctggatc aggccgcgtt tttccagttg ttgcagttgc cgggtcatgg cgccggaggt
     1621 gagcatcagc gaagaaaaca gcacggtggg ggccaggcga tagggttcac cgctgcggcg
     1681 cagggtggcc agcaggtcga attcccagct ggccagttgg tggcgggcaa agactgtatc
     1741 caggcgtttt tgcacttgaa cggcgctgcg tctgaggcgg cccaccgggc ccattggcgt
     1801 gacgtcaagg tcggggcgtt cgcgccgcca ttgttgcagg atggtggcga ctgggtcgtt
     1861 gggctggctc atgggggctc ctggggcggg gaaattatct tgcacgtgag ataaaaagcc
     1921 attaggctgt attatcttac acaaaagata gtttgccatg tttcctgtga tgtccgccag
     1981 cgcgcgcgac gcgctgctca cggcctgtgc gccgttgatt tggggttcaa cgtattgggt
     2041 caccagccag tggctgccgc cggatcggcc gttgacggcg gcggtgtggc gggtgctgcc
     2101 ggccgggctg ctattgctgc tgtgggcgcg gcagtggccg gcggccgggc agtggcggcg
     2161 ggtgttcggc ctggcggtgt tgaatatcgc cgtgtttcag gcgctgttgt ttgttgccgc
     2221 gtatcgtctg cccggcgggg tggcggcgat tctgggcgcc acgcagccct tgctggtgct
     2281 gggctgggcc tggttgctgg atggccagcg gccggcgggg gcggtgacca cggcagcggt
     2341 gctgggggtg gccggcatgg ccttgctgct gaatgcgcca ggcgcgcagt gggatacggt
     2401 gggagtgctg gcggcggcgc tgggcgcggc gtcgatggcc tgcggcagtt atctggccgg
     2461 gcgttggcag ggttgccagt tgccggtgct ggcgctgacc ggctggcagc tgacgctggg
     2521 cgggctgtgc ctggcgccgc tggcctgggc gtgggagccg cccttgccgg cgctcagcct
     2581 gacgcaaggg ctggcgtatg ggtatttatc gctgtttggc gcgctgctgg cctatgtgct
     2641 gtggtttcgc ggcattgtcc gcctgccggc ggtggcggtg tcgtcgctga gcttgctcag
     2701 cccgctgtcg gcggcggtga ttggcgcgct ggcgctgggc cagtggctgc acggctgggc
     2761 gctggccggc tggttgctca ccctgggcag tgtgctgggc gtgcagcggc tcagtcagcg
     2821 ccgccgctcg cgccagtagc tgagcaagcc atggccggaa tacaaggcca gcgcggtcca
     2881 gatcaggcca aaaccgacgg cgcgcgcagc gccaaacggc tcgtggtata gccacacgcc
     2941 cagcaagagc tgcatggtcg ggccgatgta ttgaatcagc ccgaccgtgg ccagcttcag
     3001 ccggcgcgcg ccgctggcga acagcagcaa gggcacggcg gtgaccacgc ccgcgcccat
     3061 cagcaaggca tccacggtca ggccgtgctg gccaaacgca ccttgcccct gccaatgaaa
     3121 ccaggccagc gccagcgcgg ccagcggcgc cagcaaaaag gtttccagcg ccaggccctc
     3181 cagcgagggc agcggcgctt gcttgcgcag caggccatac acgccaaaac tggcggccag
     3241 cgacagcgcc acccacggca aggcgcccag ggacagcgtc agccaggcca cgcccagcgc
     3301 cgccaacagc accgccagcg cctgcggcgg cgacagccgc tcacccagaa acacccggcc
     3361 cagcagcaca ttgaccagcg ggttgatgaa atagcccagg ctggcttcca ccacgcggtt
     3421 ttcgttcacc gcccagatat aaatcagcca atttaccgac agcagcagcg acgaggcgac
     3481 aaacagccca agcaggcggg gctggcgcac gctgaccgcc agccaggccc aatggcgctg
     3541 ccacagcaag acgccggcga caaacaccgc cgaccagaca atccggtgac acaaaatctg
     3601 caaggccggg gtttgcccca gcggcttcca gtacagcggg aacaagcccc agataaaaaa
     3661 ggcggccatg gcaaacagca cgccacggcg ggtttcttgc agggagtcgg tcatgatggg
     3721 aatggcgcaa tctgtctgct ggctggcgga cagcgcaaaa aacatgggtt gggaatgg
//
LOCUS       DAOPYX010000289         3775 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_32223_length_3775_cov_13.416667, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000289 DAOPYX010000000
VERSION     DAOPYX010000289.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 3775)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3775)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3775
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32223_length_3775_cov_13.416667"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(496..804)
                     /locus_tag="PKN55_12265"
     CDS             complement(496..804)
                     /locus_tag="PKN55_12265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014086419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1272 domain-containing protein"
                     /protein_id="HNX72066.1"
                     /translation="MLELRPTCEHCNTPLPPDALNARICSYECTFCAECAETILGNVC
                     PNCGGGFVPRPIRPARNWKNGNCLANDPASATVKYRPADLAAHAELRARLADVPPQLR
                     "
     gene            complement(959..>3775)
                     /locus_tag="PKN55_12270"
     CDS             complement(959..>3775)
                     /locus_tag="PKN55_12270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX72067.1"
                     /translation="LDAMLLGETPAAPLAEKVIEAVEADWVAQDAGLPPSDAMLPAEP
                     AEDAGDMLELDLDALLMAELPSAAQPEPSDEVLAVDAPVLEAVAEPLAEVSLDLEAML
                     MASADAVSVPDEVLAPAAAELDLDAMLMADEPRDPVTAGLSDAMPASELTLDLDAMLL
                     DEPALVVDEAPLAEQDAAPLLEADSIESAPVLSLATDPDDDREPTPLLLDADARVPAP
                     AAAEDMLELDLPLTPPPVLEALHDDTPAPAVTAPPPAPVAVPPAPDGDVLSMLAQSLE
                     GNWLAIVQGSSMPFRTFRRGLDTLSQRLTGLSATGLPGLLDAVIELADSLPISGPSPW
                     TADAVSRALELLSDTLISLPHPSTTQRAAVQMLSRELRAGPPAPMVPVVDDDMPAEPA
                     VEPSVDSAVVDTPDTSPDTVPQATAIVEPAVLPDPAPEADHPAERMDAPVDLPAMDEG
                     DENLAELPPAEMAWPAQVLPHEQAAADLAAGDVDLAEMLAELPALTDVLEDEPSPEAL
                     AAALAHAPDIEPEPETDVVADTPEQPLADAEEGESLAGWLAERPLGEEDEARLASVIS
                     VPAADAEVLPEDDDWTQPPLEELARLAKEAPLATPEPDAEIIEPAAPAPDVVTHWPAD
                     EEAALLPGFDVLPELGSADEPAPLATSDAEPAPATTALDDAAVAAKPDVVEAPPVEDT
                     PASAEPADVPPAAEALSHSISLEDIPHLRQALGSGFPTVAQALAEPAMAPAFPNSIAV
                     DDIPLLRAGTLEALLERIEPVEETPPPAAPSAEADAASADTPAEPAAPAPEVTPLGPA
                     QLAAFFASLDAEPSEPRPDPAIDLAAFLVVAQGRVQRMVQECRAARTQGIGPGFVAAA
                     DELGHNASEAGVSAMASLAAALASALTRPLSPQAVLELATDTVAVLSVMVSQGQRQEP
                     IEPAPDMVAALEALTAKPPVRKAL"
BASE COUNT          623 a         1267 c         1284 g          601 t
ORIGIN      
        1 tcacgcatcg ggcatgatgg cgcatctcgc actattcaca ggaccccacg ccatgccgct
       61 cacatcgtgg ctggaaaatc tctcatcccc caccctgatg cgggccgggc aagctttgtt
      121 ggcgcagcag gcggtgagca tgctggcgtg tgacaccgag cagtgccagg cgcgggtgca
      181 agatggccag cacagttatc gggtcacgct ggcggaacag agcacatgcg agtgcgggac
      241 agacaatcag ggcggctgcg cgcatatggc cctggctgca acacagcgcc gccgggctga
      301 ccgcgccgcg caaagccagc aaaaccgcct caccccaggc cagcgccggg cccttgcgca
      361 cacgtctgga acaacactcc agcgccgaac tggcgcagtt gctggacacc ttgcgccaga
      421 cctatcaccg gcggcgcaac ttgatgctgc tgctgaatca agcgttttga gccagcaggg
      481 tttgctgagc ggcgcttaac gcagctgcgg cggtacatcc gccagccgcg cgcgcagctc
      541 ggcatgggcg gcgaggtcgg ccggacggta tttcaccgtg gcgctggccg ggtcgttggc
      601 caggcagttg ccgtttttcc agttgcgcgc cggccgaatc gggcgcggga caaagccgcc
      661 gccgcagttg gggcagacat tgcccagaat ggtttcggcg cattccgcac aaaacgtgca
      721 ttcatacgag cagatgcgcg cgttcagcgc gtcgggcggc agcggggtgt tgcagtgttc
      781 gcaggtgggg cgcagttcca acatggcggg ctctcggcag caaaagaaaa catcagcagc
      841 atagcccggc tgtttcgccc cacgccatgc cagccgcgga caacaggcgg acggtttgcg
      901 ccaataggca aaaaagcccc ggacggatgt ccggggcttt tttacagcgg cgagtggatt
      961 acaacgcctt gcgcactggc ggtttggcgg tcagggcttc cagcgccgcc accatgtccg
     1021 gggcgggctc gatcggctcc tgacgctggc cctggctgac catcacgctg agcaccgcca
     1081 ccgtgtcagt ggccagttcc agcacggcct gcggcgacag cgggcgggtc agtgcgctgg
     1141 ccagagcagc ggccaggctg gccatggcgc tgacgccggc ctcgctggcg ttgtggccca
     1201 gctcatcagc ggcggcaaca aaaccggggc cgatgccctg ggtgcgcgcg gcgcggcatt
     1261 cttgcaccat gcgctgcacc cggccttgcg ccaccaccag gaaggcggcc agatcaatgg
     1321 ccggatcggg gcgtggctcg gaaggctcgg cgtccagcga ggcaaaaaac gccgccagtt
     1381 gcgccggccc cagtggggtg acttccggcg caggtgcggc tggctctgcc ggcgtgtcgg
     1441 cagaggccgc atccgcctca gcgctcgggg cggccggcgg cggggtttct tccaccggct
     1501 cgatgcgttc cagcagcgct tccagcgtgc cggcgcgcaa taacgggatg tcgtccaccg
     1561 caatgctgtt ggggaaggcc ggggccatgg cgggctcagc caatgcctgg gccacggtgg
     1621 ggaagccact gcccagcgcc tgacgcaggt gcgggatgtc ttccagcgag atgctgtgcg
     1681 acagcgcctc agcggcgggt ggcacatccg cgggctcggc acttgccggt gtgtcttcca
     1741 ccggaggcgc ttcgacgaca tcaggcttgg cagcgacagc agcatcgtcc agcgcggtcg
     1801 tggccggggc aggctcggca tcgctggtgg ccagcggagc aggctcgtcg gcgctgccca
     1861 gttccggcag cacgtcaaac cccggcagca aggcggcttc ttcgtcggca ggccagtggg
     1921 tcaccacatc cggcgcaggg gcggcaggct cgatgatttc ggcgtcgggt tctggcgtgg
     1981 ccagcggggc ctccttggcc agccgggcca gctcttccag cggcggttgt gtccagtcgt
     2041 cgtcttcggg caagacttcg gcatcggcag ccgggacgct gatgacggag gccagccggg
     2101 cttcgtcttc ttcgcccagc gggcgctcgg ccagccagcc agccagactc tcaccttctt
     2161 cggcgtcagc cagcggctgt tccggggtgt cggccacgac gtcggtttcc ggttcgggct
     2221 cgatgtccgg tgcgtgtgcc agcgcggcag ccagcgcctc aggcgaaggt tcgtcttcca
     2281 gcacatcggt caatgccgga agctcggcca gcatttcagc cagatccaca tcaccggcag
     2341 ccagatcggc agcggcttgt tcgtgcggca gcacctgggc cggccatgcc atttctgctg
     2401 gcggcaactc ggccaggttc tcatcaccct catccatggc aggcagatcc accggcgcat
     2461 ccatccgctc agccgggtgg tcagcttccg gggcggggtc gggcaacacg gccggctcga
     2521 caatggccgt ggcctgcggg acggtgtctg gcgacgtgtc aggggtatca actacggcac
     2581 tgtccacgct gggttcaaca gccggttctg ccggcatatc gtcgtcaaca acaggcacca
     2641 tcggcgcagg cgggccagca cgcagttcac ggctcagcat ctgcaccgcg gcacgctggg
     2701 tggtgctggg atgcggcaag ctgatcagcg tatcactcag caattccagg gcgcggctga
     2761 cggcgtcggc cgtccatggg gacgggccgg aaatcggcag gctgtcggcc aactcaatca
     2821 ccgcgtccag caagccgggc aggccggtgg ccgacagccc agtcaggcgc tggctcaggg
     2881 tatccaggcc acggcggaag gtgcggaatg gcattgagct gccttgcaca atcgccagcc
     2941 agttgccttc cagcgactgc gccagcatgg acagcacatc gccatccggc gcaggcggca
     3001 ccgccacggg agccggcggc ggcgcagtca ccgcaggggc tggcgtatcg tcgtgcagtg
     3061 cctccagcac cggcggcggg gtcagcggca gatccagctc cagcatgtct tcggcagcgg
     3121 ccggagccgg cacgcgggca tcggcatcca gcaacaaggg ggttggctca cgatcgtcgt
     3181 ccgggtctgt ggccagcgac agcaccgggg cagattcaat gctgtcggct tccagcagcg
     3241 gggcggcgtc ctgctccgca aggggagcct cgtccacgac cagcgcgggc tcatccagca
     3301 gcatggcatc cagatccagt gtcaattcac tggctggcat ggcatcgctc aggccggccg
     3361 tcaccgggtc gcgcggttca tccgccatca gcatggcgtc cagatccagt tctgcggcag
     3421 caggcgccag cacctcgtcg ggcaccgaca cggcatcggc gctggccatc agcatggctt
     3481 ccagatcaag gctcacctca gccagaggct cggccacggc ctccagcacc ggagcatcca
     3541 ccgccagcac ctcatccgat ggctcaggct gggccgccga tggcaattcc gccatcaaca
     3601 gcgcatccag atcaagttcc agcatatcgc cagcgtcttc agcaggttcg gccggcagca
     3661 tggcgtcgct tgggggaagc ccggcatcct gcgccaccca atcggcctcg acggcttcaa
     3721 tcactttttc agccagcggc gctgccgggg tttcacccag cagcatcgca tccag
//
LOCUS       DAOPYX010000290         3763 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_32375_length_3763_cov_10.344930, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000290 DAOPYX010000000
VERSION     DAOPYX010000290.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 3763)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3763)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3763
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32375_length_3763_cov_10.344930"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1904
                     /locus_tag="PKN55_12275"
     CDS             <1..1904
                     /locus_tag="PKN55_12275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008953320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNX72068.1"
                     /translation="GHVIEVEVYSRLFEYQGRPAIIGVAVDISERRIADAKQRLSATV
                     FENTSEGILIADADARIIAVNQAFTRITGYHEAEAVGKRSRMFRVDQTGQSHHARMLA
                     ALDAEGHWQGEFLDRRKNGEWFPVWLSLSSVRDQHDHQLVTHYVAVFSDITVRKEAED
                     RLQFLANHDPLTALPNRTHFIQRLELAVAEARNTQSELAVLFIDLDRFKLINDSFGHH
                     AGDDLLRLLAARLSCSLGEEGLLARLGGDEFTVLFERVASRAHLEEVTHRLLAELSKP
                     LHLEDHELFVTASIGVSIFPSDGDDAASLLRNADMAMYRSKDAGKNTCTFFAGDMNDE
                     AFERLLMENGLRLALERQEFELHYQPQINPFTHQVEAVEALIRWRHPDLGMVSPARFI
                     PITEENGMIRQIGDWVLRTACSQLKAWDDDGVHVPRVAVNLSPRQFLRGTLTATVVEV
                     LENTGLAPGRLELEVTEGMLMQNPAESVDILRELKALGVQIAIDDFGTGYSSLSYLKR
                     FPLDSLKIDRSFVIGVPDDSDSSAIAETVVAMGRKLGLNVVAEGVETSDQCRFLRACG
                     CKLLQGYFFAKPLSADNLREFILDSVRASPALGRTTALVSLDEGRATRNNAGNKEKLD
                     KTLDSPHVE"
     gene            2079..>3763
                     /locus_tag="PKN55_12280"
     CDS             2079..>3763
                     /locus_tag="PKN55_12280"
                     /inference="COORDINATES: protein motif:HMM:NF024140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNX72069.1"
                     /translation="MKNNQPVTQNEVAFPPNVYLVSKTDLKGQITDCNDAFVQISGFT
                     REELLGKSHNIVRHPDMPMAAFKDLWRTVQAGLPWRGLVKNRCKNGDFYWVEALVTPL
                     KKNGQIIGYMSVRTPPDPQARAEAEARYRAAGVQGRLPASGRRSVSLGTRLRAAMATL
                     GVLCALLGFFGVQTLNNGDEALHAMYQHHLQPSNVINRVMFLLADNRSQIMLSLQHDP
                     GSPVVSLHDHPLDMHVQTTLKNREEINALLAQLRQLPLTAEQQTLLAHFAQTRERFWQ
                     EGVNVAREQLKNGQYHPATLTLLTRINPLYADMRRDGEALIQSLADSAARSYAERSAE
                     FDRFRWQGIGLAALALLIAGVGSFLLRRAVVRPIQHAIAHFARISEGDLTAQIDVGGR
                     DEAGMLLCNLASMQVTLKAMLDNIQRASQAIDARGVELAEHMAQVSAQSDAQQTRAAS
                     VAAATKEFSQSVQEVADSARQTADAADASRQQVQTSNADIQTSMRATQAVVRTVEDAN
                     HTIERLNQSISQIGDMTRTIADIADQTNLLALNAAIEAARAGETGRGFAVVAD"
BASE COUNT          750 a         1215 c         1148 g          650 t
ORIGIN      
        1 atggccatgt gatcgaggtg gaagtctaca gccggctgtt tgaataccag ggccggccgg
       61 ccatcatcgg cgtggcggtg gacatcagcg agcgccgcat tgccgacgcc aaacaacggc
      121 tgtcggccac ggtgttcgag aacaccagcg agggtattct gattgccgat gccgacgcgc
      181 gcatcattgc cgtcaaccag gccttcaccc gcatcaccgg ctatcacgaa gccgaagcag
      241 tgggcaaacg ctcgcgcatg ttccgcgttg atcaaaccgg gcaaagccac cacgcgcgca
      301 tgctggcggc gctggatgcc gaaggccact ggcagggaga gtttctcgac cggcgcaaaa
      361 atggcgagtg gttcccggta tggctgtcgc tgtcctcggt gcgcgaccag cacgaccatc
      421 aattggtgac tcattatgtg gcggtgttct ccgacatcac cgtgcgcaag gaagccgaag
      481 accgcctgca atttctggcc aaccacgacc cgcttaccgc actgcccaac cgcacccact
      541 ttatccagcg tcttgagctg gccgtggccg aggcgcgcaa cacccaaagc gaactggccg
      601 tactgtttat cgaccttgac cgcttcaagc tgatcaacga ctcgtttggc caccacgccg
      661 gcgacgacct gctgcgcctg ctggcggcgc gcctgtcgtg ctcgctgggc gaagaaggcc
      721 tgctggcgcg gctgggtggc gatgaattta ccgtgctgtt tgagcgggtg gcgtcgcgcg
      781 cgcatctgga agaagtcacc caccgcctgc tggccgagct gtccaagccg ctgcatctgg
      841 aagaccacga gctgtttgtc accgcctcga ttggcgtatc gatcttcccg tccgacggcg
      901 acgacgccgc cagcctgctg cgcaatgccg acatggccat gtaccgctcg aaagacgccg
      961 gcaagaacac ctgcaccttc tttgctggcg acatgaacga cgaagcgttt gagcgcctgc
     1021 tgatggaaaa cggcctgcgc ctggcactgg agcgccagga attcgagctg cactaccagc
     1081 cgcagatcaa cccgtttacc caccaggtgg aagccgtgga agcgctgatc cgctggcgcc
     1141 accccgacct gggcatggtg tcgccggcgc gctttatccc catcaccgaa gaaaacggca
     1201 tgatccgcca gattggcgac tgggtgctgc gcaccgcctg ctcgcagctc aaggcctggg
     1261 acgacgacgg cgttcatgtg ccacgggtgg cggtcaatct gtcgccgcgc cagttcctgc
     1321 gcggcacgct caccgccacc gtggtggaag tgctggaaaa caccggcctg gcaccaggcc
     1381 ggctggagct ggaagtcacc gaaggcatgc tgatgcaaaa cccggcggaa tcggtggata
     1441 ttctgcgcga actcaaggcg ctgggcgtgc agattgccat cgacgacttt ggcaccggct
     1501 attcgtcgct gtcctacctc aagcgctttc cgctggacag cctgaagatc gaccgctcgt
     1561 tcgtgattgg cgtgcccgac gacagcgaca gttcggccat tgcggaaacc gtggtggcca
     1621 tgggccgcaa gctggggttg aatgtggtgg ccgaaggggt ggaaaccagc gaccagtgcc
     1681 gcttcttgcg cgcctgcggc tgcaaactgc tgcaggggta tttctttgcc aagccgctgt
     1741 cggcggacaa tctgcgcgaa ttcatcctgg acagcgtgcg tgcctcgcct gcgctgggca
     1801 ggacaaccgc gctggtcagt ttggacgagg gccgagcgac taggaacaac gcaggcaata
     1861 aggagaaact cgataaaacg cttgattcgc cccatgtcga gtaaatatac tgactcatga
     1921 attttccgca ggcccggctt tatgccgggc ctgtgtggtc tgatgcaggc catattgcag
     1981 ccaaggtacc actctgcgca tttcaccctg tgctcaaacc ggtatttctg gcgaaatgtg
     2041 catgtcatat tgcagccatg atttcaggag tccccccatt gaaaaataac cagcccgtca
     2101 cccaaaacga agtggcgttt ccgcccaatg tctatctggt gtccaaaacc gacctcaaag
     2161 gccagatcac cgactgcaac gacgcttttg tgcagatttc cggcttcacc cgcgaagaat
     2221 tattgggcaa aagccacaat atcgtgcgcc acccggatat gccgatggcg gctttcaagg
     2281 atttgtggcg cacggtgcag gcggggctgc cctggcgggg gctggtgaaa aatcgctgta
     2341 aaaatggcga tttctattgg gtggaagcgc tggtcacccc gctgaaaaaa aacggccaga
     2401 tcattggcta tatgtcggtg cgcaccccgc cagatccgca ggcgcgcgct gaggccgaag
     2461 cgcgctaccg cgccgcaggc gtgcaaggcc ggctgccggc cagtggccgg cgcagcgtgt
     2521 cgctgggcac gcggctcagg gcggcgatgg ccacgctggg cgtgctgtgc gcgctcttgg
     2581 gcttttttgg cgtgcaaacc ctcaacaacg gcgacgaagc gctgcatgcc atgtatcagc
     2641 atcacttgca accttccaat gtcatcaacc gggtgatgtt cctgctggcc gacaaccgct
     2701 cgcaaatcat gctcagcctg caacacgacc ccggcagccc agtggtcagc ctgcacgacc
     2761 atccgctgga catgcatgtg caaaccacgc tgaaaaaccg tgaagaaatc aacgccctgc
     2821 tggcgcagct gcgccagctg ccgctgacgg ccgagcagca aaccctgctg gcgcactttg
     2881 cccaaacgcg cgagcgcttt tggcaggagg gggtgaatgt ggcgcgcgag cagctgaaaa
     2941 acggccaata ccacccggcc acgctcaccc tgctgacccg catcaacccg ctctacgccg
     3001 atatgcgccg cgatggcgag gcgctgattc agtcgctggc cgacagcgcc gcgcgcagct
     3061 acgccgaacg cagcgccgaa tttgaccgct ttcgctggca gggcatcggg ctggcagcgc
     3121 tggcgctgct gattgctggc gtgggcagct tcctgctgcg ccgcgccgtg gtgcgaccga
     3181 ttcagcacgc cattgctcac tttgcgcgca tcagcgaagg cgacctcacc gcgcagattg
     3241 atgtgggcgg ccgcgacgaa gccggcatgc tgctgtgcaa tctggccagc atgcaggtga
     3301 cgctgaaagc catgctggac aatatccagc gcgcttcaca agccatcgac gcgcgcggcg
     3361 tcgagctggc cgagcacatg gcccaggtca gcgcgcaatc tgacgcgcag caaacccgcg
     3421 ccgccagcgt ggccgccgcc accaaagaat tcagccagtc ggtgcaggaa gtggccgaca
     3481 gcgcccggca aaccgccgac gccgccgacg cctcgcgcca gcaggtgcag accagcaatg
     3541 ccgacatcca gaccagcatg cgcgccaccc aggccgtggt gcgcaccgtc gaagacgcca
     3601 accacaccat cgagcggctg aatcaatcca tcagccagat tggcgacatg acgcgcacca
     3661 ttgccgacat cgccgaccag accaatctcc tggcgctgaa cgccgccatc gaggccgccc
     3721 gcgccggcga aaccggccgg ggctttgccg tggtggccga cga
//
LOCUS       DAOPYX010000291         3761 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_32396_length_3761_cov_8.093362, whole genome shotgun sequence.
ACCESSION   DAOPYX010000291 DAOPYX010000000
VERSION     DAOPYX010000291.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 3761)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3761)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3761
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32396_length_3761_cov_8.093362"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..460)
                     /locus_tag="PKN55_12285"
     CDS             complement(<1..460)
                     /locus_tag="PKN55_12285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014086125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNX72070.1"
                     /translation="MMHAKGLLKTFNPGTPIENRVLRGLDLHIETGQFVTVIGSNGAG
                     KSTFLNAISGDLLVDSGSLHLDGQDVTRQNAWQRAGLVARVFQDPMAGTCEALTIEEN
                     LALAWQRGERRGLGRAVKAELRELFREKLSILKLGLENRLTDRIGLLSGGQ"
     gene            complement(457..1359)
                     /locus_tag="PKN55_12290"
     CDS             complement(457..1359)
                     /locus_tag="PKN55_12290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018747879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNX72071.1"
                     /translation="MSLISLMGALEIGLIFALVALGVLISFRILNFPDLTADGSFPLG
                     GAVAAIMIAGGQSPWLACAAAMLAGAAAGWVTAWLNVRLNILQLLASILVMIALYSIN
                     LRVMGAPNVALIGSPTVFSPLIGDASERAMWVQPGVLAAIVVAAKLLLDAYFASQSGL
                     AMRATGANPRMARAQGISTNRAILTGMALSNALIALGGALYVQTQGGADISMGVGTIV
                     VGLAAVIIGETLIPSRSLWVITLATVLGAILYRLFIALALSADFIGLQAQDLNLVTAV
                     LVGLALVLPTLKAKLRKTVKGNPA"
     gene            complement(1547..2524)
                     /locus_tag="PKN55_12295"
     CDS             complement(1547..2524)
                     /locus_tag="PKN55_12295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009115813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNX72072.1"
                     /translation="MTLISRRIALNCLAASVLAALAPLSHAAEMKSVAVTAIVEHPAL
                     DAARKGVEDELKANGFEPGKQIKYQFQSAQGNAATAGQIARKFVGDNADVIVAIATPS
                     AQAAVAATKTVPVVFAAVTDPVAAKLVSSWKASGTNVTGVSDMLSLPPQIDLIEKVKP
                     GAKRIGMVYSPGEVNSVTVARELKAELAKRNMTLVEAAAPRTVDVAPAAKSLVGKVDA
                     IYTSTDNNVVSTYESLVRVATDAKIPLIAADTDSVKRGAIAALGMNYYDMGRQTGKIV
                     VRILKGEKAGDIAPQLGDKTSLTVNPEAAKKQGAPLSPALLKEAKDTVK"
     gene            3271..>3761
                     /locus_tag="PKN55_12300"
     CDS             3271..>3761
                     /locus_tag="PKN55_12300"
                     /inference="COORDINATES: protein motif:HMM:NF012573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX72073.1"
                     /translation="MASISSTISYTLAPSDDVLYLTGTDNINGNGNYNANIVYGNAGN
                     NIINGLQGADTVYGGAGNDTIYANMPGSLSATWQDVLYGGTGDDALYADGFDRLYGED
                     GNDSLYDGNTTSNQLYGGLGNDAYYLNNVSSVVNEQAGQGVDTVYSTVSVALDNSLAG
                     APL"
BASE COUNT          738 a         1110 c         1196 g          717 t
ORIGIN      
        1 gctggccgcc cgacagcagg ccaatgcggt cggtcaggcg gttttccagg cccagcttga
       61 ggatggacag tttttcgcga aacagctcgc gcagctcggc cttcaccgcg cggcccaggc
      121 cgcgcctttc gccgcgctgc caggccaggg ccaggttttc ttcgatggtc agcgcttcgc
      181 aagtgccggc catcgggtcc tgaaacaccc gcgccaccag cccggcgcgc tgccaggcgt
      241 tttgccgggt gacatcctga ccgtccaggt gcaggctgcc gctgtccacc agcaaatcgc
      301 cgctgatggc gttcagaaag gtggatttgc cggcgccgtt gctgccgatc acggtgacga
      361 actggccggt ttcgatgtgc aggtccagcc cgcgcagcac gcggttttca atcggcgtgc
      421 cggggttgaa tgtcttgagc agacctttgg cgtgcatcat gccggattcc ccttcacggt
      481 tttgcgcagt ttggctttca gcgtcggcag caccagcgcc aggcccacca gcacggcggt
      541 gaccagattc agatcctgcg cttgcaggcc gatgaagtcg gcgctcagcg ccagggcgat
      601 gaacaggcga tacaaaatgg cgcccagcac ggtggccagg gtgatcaccc acagcgagcg
      661 cgacggaatc agcgtttcgc cgatgatcac cgccgccagg ccgaccacaa tggtgcccac
      721 gcccatggaa atatccgctc cgccctgggt ttgcacatac agcgcgccgc ccagcgcaat
      781 cagcgcattg gacaaggcca tgccggtgag gatggcgcgg ttggtggaaa tcccctgggc
      841 gcgcgccatg cgcgggttgg cgccggtggc gcgcatggcc aggccggatt gcgaggcaaa
      901 ataagcgtcc agcagcagct tggcggccac cacgatggcg gccagcacgc ccggctgcac
      961 ccacatggcg cgctcggagg cgtcgccaat cagcgggctg aacaccgtcg gcgagccgat
     1021 cagcgccaca ttgggcgcgc ccatcacccg cagattgatc gaatacaggg caatcatcac
     1081 cagaatgctg gccagcagtt gcaggatgtt cagccgcaca ttcagccagg cggtcaccca
     1141 gccagccgcg gcgccggcca gcatggcggc ggcgcaggcc agccacggcg actggccgcc
     1201 ggcgatcatg atggccgcca ccgcgccgcc cagcggaaag ctgccgtcgg cggtgaggtc
     1261 cggaaaattg aggatacgga acgaaatcag cacgcccagc gccaccaggg cgaagatcag
     1321 gccgatttcc agcgctccca tcagggagat gagtgacatg gattacctcg acaaaaacga
     1381 acaccgcgcc cggtggggcg cggcagactg ccgccctgcg gcgcggggtg gcgcaccggg
     1441 gcggctcggc tgagcttaag ccgcgcaggg cggcgccgga caaaccggcg ccctgcgggc
     1501 ggtcaggtcg cgctgggcat caggtggcgg cccagcgcgt cagccatcat tttaccgtgt
     1561 ctttggcttc tttgagcagg gccggcgaca gcggcgcgcc ttgcttcttg gcggcttccg
     1621 ggttgacggt cagcgaggtc ttgtcgccca gttgcggggc gatgtcgccg gccttttcgc
     1681 ccttgaggat gcgcaccacg atcttgccgg tctggcggcc catatcgtag tagttcatgc
     1741 ccagcgcggc gatggcgccg cgcttgaccg agtcggtgtc ggcggcgatc agcgggatct
     1801 tggcgtcggt ggccacgcgc accagcgatt cataggtgga caccacatta ttgtcggtgg
     1861 aggtgtaaat ggcgtccacc ttgcccacca ggctcttggc ggccggggcc acgtccacgg
     1921 tgcgcggcgc ggcggcttcc accagggtca tattgcgctt ggccagctcg gctttcagtt
     1981 cgcgggcgac ggtgaccgag ttgacttcac ccgggctgta caccatgccg atgcgcttgg
     2041 cgcccggctt gaccttttcg atcaggtcga tttgcggcgg cagcgacagc atgtcggaca
     2101 cgccggtgac attggtgccg ctggccttcc agctgctcac cagcttggcg gccaccgggt
     2161 cggtgacggc ggcgaacacc accggcacgg ttttggtggc ggcgacggcg gcctgggcgc
     2221 tgggggtggc gatggcgacg atcacgtcag cattgtcgcc cacaaacttg cgggcgatct
     2281 ggccggcggt ggcggcgttg ccctgcgcgc tctggaattg gtatttgatt tgtttgcccg
     2341 gttcaaagcc gttggccttg agttcgtctt ccaccccctt gcgggcggcg tccagcgccg
     2401 ggtgttcgac gatggcggtc accgccaccg acttcatttc agcggcgtgg ctcagcgggg
     2461 ccagggcggc cagcacgctg gcggccagac agttcagggc gatacggcga gagataagcg
     2521 tcatgatgct tccgtgcggt gagcaagtgc tcttgcgtga aggtttacgg gaagacgacg
     2581 gccgggcgcg gcgtgggcag gccgacagcg cggctgtcgc gtaacagggc aatcttattg
     2641 caaattcatg aacgcaactg gcaaaaacga gaaatataat ttcacaaata tgacatttag
     2701 cgcaataaac aaaaagataa acagaaaaaa cgattgtttt aaccgaggat tgcattgctt
     2761 tgcgccatat cctgccgatt ttttgcctgc cgccttgagt ggtttctgcc tggaacaccc
     2821 tggttttcga tgaatttatg gcgcagaacc aaacgactga ccgacagtgc accacggcca
     2881 gcaggctgct ggtgcagcgt ggtgcgccgg ctttgtggct tttgtgcaac gcaaaaccgg
     2941 cctgcaagcg gatcgtacac cgcacaaggc aacgacaatg atcattgtat taacattaat
     3001 cggggtttaa cccgatgttc tacttgccat ttctgctgat ggccggcgcg tcacctgcat
     3061 ggcaaaaatg gtccgtgcgc ggggtatggt tgacagattt tttatgcgtc ccacctatgt
     3121 ttatataaaa aacacacaac agaaaatcgg caaatttcat gattaccggc aggtaacgca
     3181 tgtaagctga tttctggctt gaatactgca caacgccatg ggtacagtga aatcgcgggc
     3241 caaccgcaga taacacaaaa ggataagatc atggcaagca tcagcagcac tatcagctat
     3301 accttggcgc cgagtgatga cgtgctgtac ctgacaggta cagacaatat caatggcaat
     3361 ggcaactaca atgccaatat tgtttatggc aatgccggaa acaatatcat caatgggttg
     3421 caaggtgccg acaccgtgta cggcggtgct ggcaatgaca cgatttatgc caatatgcct
     3481 ggcagcctga gcgccacctg gcaggatgta ctgtacggtg gcacaggtga tgatgccctg
     3541 tatgccgatg gttttgatcg tttgtatggc gaagatggca acgacagcct gtacgacggc
     3601 aacaccacca gcaatcaact gtatggcggc ctgggcaatg atgcctatta cctgaataac
     3661 gtctccagcg tggtgaatga gcaagctggc cagggcgtgg acacggtgta cagcacggta
     3721 tcggtggcgt tggataattc acttgctggc gcaccgctga t
//
LOCUS       DAOPYX010000292         3756 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_32467_length_3756_cov_10.807349, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000292 DAOPYX010000000
VERSION     DAOPYX010000292.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 3756)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3756)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3756
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32467_length_3756_cov_10.807349"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2135)
                     /locus_tag="PKN55_12305"
     CDS             complement(<1..2135)
                     /locus_tag="PKN55_12305"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNX72074.1"
                     /translation="MKHSKKWGVQAKILLVFTAINILATAAYTLFVYQIKTDSIYEAT
                     DARLTAAAHAVTRMLPADYLEQPLNTAPPANYLQQTRSLYAYSQEAGLRYVYAFVEDH
                     GQVRYLADSASADEIRRDKYGQYMQAYREPPPALWQAFRDGQPHFAEYQDQFGQFRSL
                     FVPGERRDGLRYVIGADIDVAYLQNRSHEALLTSLLIGGAIFAISLALSWWVARALTQ
                     PVRALLSVTERLADGDYSARAHSHGQDEVAQLAGGLNAMGQAIAQREEAMLQLIYRDE
                     LTGLGNRLALDEALTAALSQHQMGVLMLVHLDRFRAINDLLGFAAGDLVLQVATTRLS
                     ELWPDDSRVHRLGGNVFALLVTGLPAYDSDSLHRQVFRALEGEPFTLADQQIDLTVTL
                     GIVRFPQHGNQAGLLLRNAEVALYAAKQAHKPWSEYNAEQVGSRREQLSLLGELRRAV
                     EQHEMHVFYQPQVSINQGQLVQAEALVRWRHPERGWVPPSAFIPFAEQTGRIRSLTDW
                     LLNEVAQQTTAWRASGQPLTISINVAVHDLEDPDFPKRVAAALRRHDAQPQDLCLEIT
                     EGGLMADPEQARATLTALHEAGHRLAIDDFGTGYSSLAYLSHLPVDELKIDRSFIMDL
                     NLHNRSIVLSTISLGHALGLKVVAEGVETGNQYRELALLGCDIAQGYLIAKALPLEEF
                     NAWRHAHPHECDWGSCPDAAPDLPPGGVTR"
     gene            complement(2262..>3756)
                     /locus_tag="PKN55_12310"
     CDS             complement(2262..>3756)
                     /locus_tag="PKN55_12310"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX72075.1"
                     /translation="FSQDELCQMARQRLDALPIHHAHEWDYERALADALPPDAPERYT
                     LRWRLLKDLQDCRARLHEFFDFNVEDRDFKDAADYRQRLRALPEQLRRCPHAKTEEEK
                     QQWVDAVMGSALLDLVVKEQIAQETPAMDQGLFRRIQDSLAKNHKILEESMSKSDAKV
                     FFKAILTHDFFPDCPDSKLAYNQIRTCFNQHPTALETASKFLINSSHHDLWCLKTIEM
                     LEQYFPKLSQLSLWKGQLLHIYLNQPDEAEMAYRQAVLLDKDNSTAHLLLGIFLLHQQ
                     KDFHAAETALRKAISLEATNSLAWTFLGFMLEHEKNNYVEAEHAYRQAINLSGHSRLA
                     KSCLAPLLCKQEKWPEAKSLYREILEMPFNTEKADNAEKIRFYETSLQAHLCLANRQM
                     AVDILQALAQQAEQGDNVAFDTLKLQTLECHDLGLGERLADWMSECEQAGFLLPFTQA
                     LYTLAGAQARLQDVPQETRQMAEEIIRLARQRATRIPPTPTSSTERT"
BASE COUNT          697 a         1053 c         1173 g          833 t
ORIGIN      
        1 cgcgtcaccc cgcccggcgg caaatccggc gcggcgtccg ggcagctgcc ccagtcgcat
       61 tcgtgggggt gggcgtggcg ccaggcgttg aattcttcca gcggcagcgc tttggcgatc
      121 agataaccct gggcgatgtc gcagcccagc agcgccaact cgcggtattg attgccggtt
      181 tccacgcctt cggccaccac tttcagaccc agcgcatggc ccagactgat ggtggacagc
      241 acaatcgagc ggttgtgcag gttcagatcc atgatgaagc tgcggtcgat tttcagctcg
      301 tccaccggca gatggctcag gtaggccagc gaggagtagc cggtgccaaa gtcgtcaatc
      361 gccagccggt ggccggcctc gtgcagggcg gtgagggtgg cgcgggcttg ttccgggtcg
      421 gccatcagcc cgccttcggt gatttccagg cacaaatcct gtggctgggc gtcgtggcgg
      481 cgcagggcgg cggccacccg cttgggaaag tccgggtctt ccaggtcatg cacggcgacg
      541 ttgatcgaga tggtcaacgg ctggccgctg gcgcgccagg cggtggtttg ctgggccact
      601 tcgttcagca gccagtcggt cagcgagcgg atgcggccgg tttgctcggc aaacgggata
      661 aacgccgacg gcggcaccca gccgcgctcc gggtgacgcc agcgcaccag cgcctcggcc
      721 tgcaccagct ggccttggtt gatgctgacc tgcggctgat aaaacacatg catttcgtgc
      781 tgctcgaccg cgcggcgcaa ttcgcccagc agcgacaatt gctcgcggcg gctgcccacc
      841 tgctcggcgt tgtattcgct ccacggtttg tgcgcctgct tggcggcgta cagcgccacc
      901 tcagcgttgc gcagcagcag cccggcctga ttgccgtgct gcggaaagcg cacaatgccc
      961 agggtgacgg tgaggtcgat ctgctggtcg gccagggtga acggctcgcc ttccagcgcg
     1021 cgaaacacct ggcggtgcag gctgtcgctg tcgtatgccg gcaggccagt caccaacagg
     1081 gcaaacacat tgccgcccag ccgatgcacg cggctgtcgt cgggccacag ctcggacagg
     1141 cgggtggtgg ccacttgcag caccaaatcg ccagcggcaa agcccaataa atcgttgatg
     1201 gcgcgaaagc ggtccagatg caccagcatc agcacgccca tctggtgctg gctgagcgcc
     1261 gcagtcagcg cctcgtccag cgccagccgg ttgcccaggc cggtgagctc atcacggtaa
     1321 atcaattgca gcatggcttc ttcgcgctgg gcaattgcct ggcccatggc gttcagcccg
     1381 ccggccagct gggcgacttc gtcctgccca tgcgaatgcg cgcgcgcgct gtagtcgcca
     1441 tcggccagcc gctcggtgac cgacagcaag gcgcgcaccg gctgggtcag cgcccgcgcc
     1501 acccaccacg acaaggccag cgaaatggca aaaatcgcgc cgccaatcag cagcgaggtc
     1561 agcagcgctt catggctgcg gttttgcaga taagccacgt caatgtcggc gccaatcaca
     1621 tagcgcaggc catcgcggcg ctcgccgggc acaaacagcg aacggaactg gccaaattga
     1681 tcctggtatt cggcaaagtg cggctgaccg tcgcggaatg cctgccacaa tgccggcggc
     1741 ggctcgcggt aggcttgcat gtactggccg tatttatccc ggcggatttc gtcggccgag
     1801 gctgaatcag ccagatagcg cacctggcca tggtcttcaa caaaggcgta cacatagcgc
     1861 agcccggctt cctggctgta ggcataaagc gaacgggttt gctggagata attggccggc
     1921 ggcgcggtgt tgagtggctg ttccagataa tcggcaggca gcatgcgggt cacggcatgc
     1981 gccgctgcgg tcagccgggc gtcggtggcc tcgtagatgc tgtcggtttt gatctggtag
     2041 acaaacaggg tgtaggccgc ggtggccagg atattgatgg cggtgaacac cagcaggatc
     2101 ttggcctgca cgccccattt tttgctgtgt ttcatctgcc tttcccgtgc gtcggtggac
     2161 atgccgacgc gcatgccggc tgggaagttt agcacgttgg ggtggcttgg ccagtgtggg
     2221 cgcgccaggc cgggctgcgt gcagaggccc ggcctggcgg attacgtccg ctcagtggat
     2281 gacgtcggcg tgggcggaat gcgagtggcg cgctgccgag ccaggcggat gatttcttcc
     2341 gccatctgcc gggtttcctg cggcacatcc tgcaagcgcg cctgtgcacc ggccaaggtg
     2401 tacagggcct gggtgaacgg caggagaaag ccggcttgct cacactcgct catccagtcg
     2461 gccagacgct cgccaaggcc aaggtcatgg cattcgaggg tttgcagttt tagggtatcg
     2521 aatgcgacgt tatctccttg ctcggcttgt tgagccagcg cttgcagaat atctactgcc
     2581 atttggcgat tggctagaca gaggtgggct tgtaaagacg tttcgtagaa gcgaattttc
     2641 tctgcgttat ctgctttctc tgtattaaat ggcatctcta agatttcacg atacagtgat
     2701 ttagcttctg gccatttttc ttgcttacat aggagtggtg caagacagga tttggctaat
     2761 ctgctatgcc cgcttagatt gatcgcttgg cgatatgcgt gttctgcctc tacatagtta
     2821 ttcttttcgt gctcaagcat gaatcctaga aaggtccatg ctaaagaatt ggttgcttct
     2881 agtgaaatgg cttttcgtaa ggctgtctca gcggcatgaa aatctttttg ttggtgtaaa
     2941 aggaatatgc ctaaaaggag atgggcagtt gaattatctt tatcaagcaa aactgcttgg
     3001 cggtaagcca tttcagcctc gtcaggctgg tttaggtaga tgtgaagcag ctggcctttc
     3061 caaaggctca actggctcag ctttggaaaa tattgttcta acatttctat ggtttttagg
     3121 caccataagt catggtgtga tgagttgatt aaaaatttag aggctgtttc tagtgctgtc
     3181 gggtgttggt tgaagcaggt gcgaatctga ttataggcta attttgagtc agggcagtcc
     3241 ggaaaaaaat catgggttaa gatggcttta aaaaaaactt tggcatcact cttgctcatg
     3301 ctttcctcta gaatcttgtg gtttttagct agtgagtcct gtatccgcct aaataggccc
     3361 tgatccatcg ccggagtttc ttgtgcaatt tgctctttta ctaccaagtc caagagtgcc
     3421 gatcccatca ccgcatccac ccactgctgc ttttcctctt ctgtttttgc atgcgggcaa
     3481 cggcgcagtt gttccggcag ggcgcgcagc ctttgccggt aatccgctgc atctttgaaa
     3541 tcccggtctt cgacgttgaa atcaaaaaat tcatgcaggc gggcgcggca gtcttgcagg
     3601 tctttgagca gacgccagcg caaggtgtag cgttcgggtg cgtccggcgg cagggcgtcg
     3661 gccagggcgc gttcgtagtc ccattcatgg gcgtggtgga tgggcagcgc atccaggcgc
     3721 tgtctggcca tctggcacag ctcgtcctgg ctgaac
//
LOCUS       DAOPYX010000293         3752 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_32513_length_3752_cov_12.232892, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000293 DAOPYX010000000
VERSION     DAOPYX010000293.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 3752)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3752)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3752
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32513_length_3752_cov_12.232892"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(3..902)
                     /locus_tag="PKN55_12315"
     CDS             complement(3..902)
                     /locus_tag="PKN55_12315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008954713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNX72076.1"
                     /translation="MELRSPRLWGTVCVLVSAAAFGSMAIFAKLAYAAGLDAASLLFL
                     RFTLAGLCLLPVVLIGRLPWPRGRALVALLVMGGVCYTGNSQTFFMALNYAPAGTVAL
                     LLYLYPVLVMLLSAWLFGEQLTARKWLAAALTFSGLVITLGLELAGQPLGIALGVASA
                     LIYSSYILLGGRYATGSHPISSACVVFLAASLTNGLIVLAKGDGLHWPHNSAGWLALL
                     AIALICTVLAIVGFLVGLRWVGATRASMMSTFEPVVTVLLAAVWLGESVSMSQLLGGA
                     LILAGVLVLSRPQAEVEVSDIHD"
     gene            1265..1960
                     /locus_tag="PKN55_12320"
     CDS             1265..1960
                     /locus_tag="PKN55_12320"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HNX72077.1"
                     /translation="MSDFDLFAMETLAADASRQVLANPHASAADYRQALGTLLTHFER
                     LVRETRRMISQSDRREAEMNQLNARLGELARRLQYEATHDGLTGVLNRAAITDRAEQL
                     MGAVILLDIDHFKRINDHHGHPCGDATLIELTRRVLATLPAEAMLGRLGGEEFLVVCP
                     GMRLGEAAILAETVREQVAGQPFTAGGHALAVSISAGVALLMADAHSAYQSADAALYR
                     AKSGGRNRVEVAR"
     assembly_gap    2004..2103
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2389..>3752
                     /locus_tag="PKN55_12325"
     CDS             2389..>3752
                     /locus_tag="PKN55_12325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007341564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HNX72078.1"
                     /translation="MTASAERTEHDLLGARTLPGHAYYGIHTLRALENFPISGAPISV
                     YPELIRALACVKQAAAEANAELGLLPEAKRRAIVAACAEIRAGALHEHFVVDVIQGGA
                     GTSTNMNANEVIANRALELMGHQRGDYQHLHPHQDVNLSQSTNDVYPTALKLAAYFGI
                     SRLREAMAYLRQAFADRAEAFADVLKMGRTQLQDAVPMTLGQEFGAYAVMLGEDEARL
                     GEASLLITEINLGATAIGTGINTHPDYAHVACRHLARISGVPVSTADNLVEASQDCGS
                     FVQLSGVLKRVAVKLSKTCNDLRLLSSGPRAGLGEINLPTTQAGSSIIPGKVNPVIPE
                     VVNQIAFEVIGNDLTVSFAAEAGQLQHNAFEPIIAHSLFKSITHLRNGCLVLADRCVR
                     GITANREHMRAQVENSIGIVTALTPYIGYEQATELAQDALQTGRSVYKLALAKGLLTR
                     EALE"
BASE COUNT          734 a         1224 c         1117 g          577 t
ORIGIN      
        1 cctcaatcat gaatatcact gacctcaacc tcggcctgcg gccgcgacaa caccagcacg
       61 ccggccagga tcaaggcgcc acccagcagt tgcgacatgc tcaccgattc gcccagccac
      121 accgccgcca gcagcacggt gaccaccggt tcgaaagtgg acatcattga ggcgcgggtg
      181 gcgcccaccc agcgcagccc gaccaaaaac ccgacaatcg ccagcacggt gcaaatcagc
      241 gcaatcgcca gcagcgccag ccagccggcg ctattgtgcg gccagtgcag gccatcgccc
      301 ttggccagca caatcaggcc attggtcagg ctggcggcca gaaacaccac gcaagccgaa
      361 gaaatcgggt ggctgccggt ggcgtagcgc ccgcccagca agatatagct ggaataaatc
      421 agcgccgagg ccacgcccag ggcaatgccc agcggctggc cggccagctc cagccccagg
      481 gtgatcacca ggccgctgaa ggtcagcgcg gcggccagcc atttgcgcgc ggtaagctgc
      541 tcgccaaaca gccaggccga cagcagcatc accagcaccg gatacagata cagcagcaag
      601 gccacggtgc cggccggggc gtaattgagc gccatgaaaa aagtttgcga attgccggtg
      661 tagcacaccc cgcccatcac cagcagcgcc accagcgccc gcccgcgcgg ccacggcagc
      721 cggccaatca gcaccaccgg caacaggcac agcccggcca aagtaaaacg caaaaacaat
      781 aaagacgcgg catccaggcc ggcggcgtaa gccagcttgg caaaaatcgc catgctgcca
      841 aaggcggcgg cggacaccag cacgcacacc gtgccccaca gacggggcga acgcaattcc
      901 atgatgtcag gctccagcaa gcagggctgt ggcggcgggc gcgcgccttg tggtgcgcaa
      961 attattagac agccgtcaca accattatcg ggcgaacagc ctagcacagc actgcgccag
     1021 cggcagcatc tgtgcagtaa aacacccgaa accagcagag acagcgcaca gttttggtga
     1081 aaacacccag gcagatcggg tgttgcaccg gggcagcagc gcaaaaatct gatgaaaatc
     1141 ttcaacactg ctggcatgcg acaggcagat gacatgcaac cgccctgtca tgtaaccgtc
     1201 atcgccctgc gctatgctgt ttgccccacc tttgctatcc tcgcgccccg gcgcgccatg
     1261 cccaatgtct gatttcgacc ttttcgccat ggaaaccctg gctgccgacg ccagccggca
     1321 agtgctggcc aatccgcacg ccagcgcggc cgattatcgc caggccttgg gcacactgct
     1381 gacgcatttc gagcggctgg tgcgggaaac ccggcgcatg atcagccagt ccgaccggcg
     1441 cgaggccgag atgaaccagc tcaacgcccg cctgggcgag ctggcccgcc gcctgcaata
     1501 cgaagccacc catgacgggc tgaccggcgt gctcaaccgc gcggccatca ccgaccgcgc
     1561 cgagcaattg atgggcgcgg tgattctgct ggacatcgac cacttcaaac gcatcaatga
     1621 tcaccacggc cacccctgcg gcgacgccac gctgattgag ctcacccgcc gggtgctggc
     1681 cacactgccg gcggaagcca tgctgggccg gctgggcggc gaggaatttc tggtggtgtg
     1741 ccccggcatg cggctgggcg aggcggcgat tctggcggaa accgtgcgcg agcaggtggc
     1801 cggccagccg ttcactgccg gtggccacgc gctggcggtc agcatcagcg ctggcgtggc
     1861 cctgctgatg gccgatgcgc acagcgccta tcaaagcgcc gacgccgcgc tgtatcgggc
     1921 caagagcggc gggcgcaatc gggtggaagt ggcgcgctga gcgggccggc ggcttacccc
     1981 ggtatgctgg gcacggtgga caannnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2101 nnncggccaa tatgccagat cgtcagcgct gctcagcgtg gctgttggcg cgccgggcat
     2161 gggcagccca tggggtgcgc cgttttgcgc aatccgctat ccttctgcct tgattttcag
     2221 gctgcgatgc tgacaaatac ttgtttttac aatggctttt gttgcaccgc accagcctga
     2281 actatgccgc tgcggcatgg cgtgatgaac aaacagcatt ggtcagccat ttgtgcgtgt
     2341 cgctatagtc cgcagcctgt accgcctgac aggattcgat ttcactccat gactgcttct
     2401 gccgaacgca ccgaacacga tctgctgggc gcgcgcacgc tgcccggcca cgcctattac
     2461 ggcattcaca ccctgcgcgc gctggagaat tttccgatca gcggcgcgcc gatttcggtg
     2521 tatcccgagc tgattcgcgc gctggcctgc gtcaagcaag ccgccgccga agccaatgcc
     2581 gagctgggct tgctgcccga ggccaagcgc cgggcgattg tcgccgcctg cgccgaaatt
     2641 cgtgccggcg cgctgcacga gcattttgtg gtggatgtga tccagggcgg cgccggcacg
     2701 tccaccaata tgaacgccaa cgaagtcatc gccaaccgtg cgttggagct gatgggccac
     2761 cagcgcggcg actatcagca cctgcacccg catcaggacg tcaacctcag ccagagcacc
     2821 aatgatgtct accccaccgc gctgaagctg gccgcctact ttggcatcag ccgcttgcgc
     2881 gaggcgatgg cctatctgcg ccaggcgttt gccgaccgcg ccgaggcgtt tgccgatgtg
     2941 ctgaaaatgg gccgcaccca gttacaggac gcggtgccga tgacgctggg ccaggagttt
     3001 ggcgcttatg cggtgatgct gggcgaggac gaagcccggc tgggcgaggc gtctttgctg
     3061 attaccgaaa tcaatctggg cgccacggcc attggcaccg gcatcaacac ccacccggat
     3121 tacgcccatg tcgcctgccg ccatctggcg cgcatcagcg gcgtgccggt gtccaccgcg
     3181 gataatctgg tagaagcctc gcaggattgc ggcagctttg tgcagctctc cggcgtgctc
     3241 aagcgggtgg cggtgaaact gtccaaaacc tgcaacgacc tgcgcctgtt gtcatccggc
     3301 ccgcgcgccg gcctgggcga aatcaacctg ccgaccactc aggccggctc cagcatcatt
     3361 cccggcaagg tcaacccggt gatcccggaa gtggtcaacc agattgcttt tgaagtgatt
     3421 ggcaatgacc tgacggtgag ctttgccgcc gaggccggcc agttgcagca caacgccttc
     3481 gaaccgatca tcgcccacag cctgttcaag agcatcaccc acttgcgcaa tggctgcctg
     3541 gtgctggccg accgctgcgt gcgcggcatc accgccaacc gcgagcatat gcgcgcgcag
     3601 gtggaaaact ccatcggcat tgtcaccgcg ctgacgccgt atatcggcta tgagcaggcc
     3661 accgagctgg cgcaggatgc gctgcaaacc gggcgcagcg tgtacaagct ggcgctggcc
     3721 aagggcttgc tcacccgcga ggcgctggaa ga
//
LOCUS       DAOPYX010000294         3743 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_32619_length_3743_cov_12.158351, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000294 DAOPYX010000000
VERSION     DAOPYX010000294.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 3743)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3743)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3743
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32619_length_3743_cov_12.158351"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            102..722
                     /locus_tag="PKN55_12330"
     CDS             102..722
                     /locus_tag="PKN55_12330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX72079.1"
                     /translation="MQVNTSASAMLPTIKKATHAEDDGKLVANSRGEFGMAFTFDPEL
                     ADTVHNGVRYFMPPSHLVEKGDAETLLKKHLSPEAWAGVKRMLDDVATGRDKLSPEEY
                     DPVNYEKQMKKQADSIFRSPAGEILAVVYKDGTIHTQGEGKRIDFDALSQSAKGLSEH
                     EYRAAMRNGIAAALGSRAVASYYDYNKPAPTMREIHEEERIFYSRG"
     gene            complement(1056..2393)
                     /locus_tag="PKN55_12335"
     CDS             complement(1056..2393)
                     /locus_tag="PKN55_12335"
                     /inference="COORDINATES: protein motif:HMM:NF019421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HipA family
                     toxin"
                     /protein_id="HNX72080.1"
                     /translation="MGRKSHRQQLNVWMNGLLVGEWTLAHGEEIFAYHASWLNHPQTR
                     PLSLSLPFLPNNAAHRGAVVNAYFDNLLPDSDIIRRRLAQHHRLSDSAPFTLLAALGR
                     DCVGALQLLPEDETPQNLQCIIGEPLDDAAIAALLQQTVQPGLPGQRNSDADLRLSIA
                     GAQEKTALLQHAGRWQRPLGSTPTTHIFKLPLGLVGGMQADMRSSVENEWLCAQLVAA
                     FGLPVARCDIAHFEAQKVLVVERFDRRLSSDGRWLMRLPQEDLCQATATPPYLKYQAD
                     GGPGIARILDILAGSENSETDRRQFFKAQVVFWLLAATDGHAKNFSLFHLPGGRYRAT
                     PLYDILSAHPVIGHGAGKVAPQKAKLAMAVRGQSNHYLIERIQPRHWLAQARSSGLTE
                     AWADELLNELADNTGEILQKIEGELPPDFPADVAETIFHGIRRQREKLTARLR"
     gene            complement(2395..2646)
                     /locus_tag="PKN55_12340"
     CDS             complement(2395..2646)
                     /locus_tag="PKN55_12340"
                     /inference="COORDINATES: protein motif:HMM:NF024949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNX72081.1"
                     /translation="MHYPILMPEQLPAMLRSLRQRAGLTQAQLAERLGISQQSYARLE
                     ARPAATSVARLSKLLRVLNAELVIHTRDDQPTAVSDEAW"
     gene            complement(2849..3637)
                     /locus_tag="PKN55_12345"
     CDS             complement(2849..3637)
                     /locus_tag="PKN55_12345"
                     /inference="COORDINATES: protein motif:HMM:NF021631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HNX72082.1"
                     /translation="MIPADIRQDILHRLQRAEQEHQVRILFAIESGSRAWGFASPNSD
                     YDVRFVYAHPRDWYLAVDLEERRDVIEYPIVDDIDLNGWDVRKALRLLWKSNPALIEW
                     LQSPITYCEHGDFRQQALALLPAIYTSERGLYHYRSMASKNYRGYLKQDEVPLKKYFY
                     VLRPLLAARWIADGRGAPPMEFERLLAMLNADEAQVRADIHTLLAQKRQAPELGRAAA
                     VPSLNAFIETELARTLPELPWPPGGATRLDPLNQLFHATLAEGG"
BASE COUNT          751 a         1026 c         1149 g          817 t
ORIGIN      
        1 tttcggtgta gtttgacctt gctgcgggca agctcgtgct taccttggcc ttgcctgcac
       61 gtttgtacac actcgctcat gtttaacctc atggaggtca tatgcaagtc aataccagcg
      121 cttcggccat gctgcctacc atcaaaaaag ccactcatgc agaggatgat ggcaaacttg
      181 tcgccaattc tcgtggtgaa tttggtatgg cattcacttt tgatcctgag ttggcagata
      241 ccgtacataa cggagttcgt tattttatgc cgccatcgca tctcgtagaa aaaggagatg
      301 cagaaacttt attgaaaaaa catcttagcc cagaggcttg ggctggtgtt aaaaggatgt
      361 tggacgatgt agcaacaggg cgagataagc tttcgccaga agaatatgat cctgtaaact
      421 atgaaaaaca aatgaagaaa caagcggata gtattttccg cagcccggca ggtgaaatcc
      481 tggctgtggt gtataaagat gggacgattc acacgcaagg cgaggggaag cgcattgatt
      541 ttgatgcact gtctcaatcc gccaaaggcc tgagtgagca tgaataccgt gccgccatgc
      601 gcaatggcat tgctgctgcc ttgggcagcc gcgcggtggc cagttattac gactataaca
      661 agccggcgcc gaccatgaga gaaattcacg aagaagagcg cattttttat agccgtggct
      721 gacgcttgct gttcacctgt tgcacattcg gagaggggca gcccacaagc atcagtggcg
      781 ctgcccgtat atctgtatcc cgcaatttca tcagggcaca cggccaagct gccgcgcccg
      841 cgccaaagct tgtctccagg gctttcgccg gggtgagtca tggtgttttc cgcatccccg
      901 gcttccagcc tgcacaagcg cctgcgtcgc atcgctcaca aaaccctagg ggcacgacca
      961 caggctgtac gggcatgcaa caactccgga ggggtttgtt ggcggcgcta acgttctgtg
     1021 cgcatcaccg agcttgtctc aggggcttgt gttgctcagc gaagccgtgc cgttaatttt
     1081 tcgcgctgac gtcgtatgcc gtgaaaaatg gtctcggcca catcggccgg aaagtctggc
     1141 ggtaattctc cctctatctt ttgcaaaatc tcaccggtgt tgtcagccaa ttcgttcaat
     1201 agctcatcgg cccacgcttc agtcagaccg ctgctgcgcg cttgcgccag ccagtggcgt
     1261 ggctggatgc gctcaatcag gtagtgattg ctttgcccgc gcaccgccat cgccagtttg
     1321 gctttttgcg gcgccacttt gccggcgccg tggccaatca ccggatgggc ggacagaatg
     1381 tcatacagcg gcgtagcacg gtaacggccg cccggcagat gaaacaggct gaagtttttg
     1441 gcgtggccgt cggtggcggc cagcaaccag aacaccactt gcgccttgaa gaactgacgg
     1501 cggtcggttt cgctattttc tgatccggcc agaatatcga gaatgcgcgc gatgccgggg
     1561 cctccatcgg cttggtattt gaggtagggg ggcgtggcgg tggcttggca caggtcttct
     1621 tgcggcagac gcatcaacca gcggccatcg ctggacaggc ggcggtcaaa gcgttcgacg
     1681 accagcactt tctgtgcttc aaagtgggcg atatcgcacc gggctactgg tagaccaaat
     1741 gcagccacca gttgcgcaca gagccattcg ttctcgaccg agctgcgcat atcggcttgc
     1801 atgccgccca ctaaccctag cggcaattta aaaatatgcg tggtaggtgt gctgcccagc
     1861 gggcgttgcc agcggccagc atgctggagc agtgcggttt tttcttgcgc gccggcgatg
     1921 gacaatcgca agtcggcatc ggaatttctc tggcctggca agcccggttg cactgtttgt
     1981 tgcagcaggg cggcaatggc ggcatcgtcc aggggttcgc cgatgataca ttgcaggttt
     2041 tgcggtgttt cgtcttctgg cagcagttgc agtgcaccca cacaatcgcg ccccagtgcg
     2101 gccagcaggg taaacggcgc actgtcgctc agccggtgat gctgcgccag acggcggcgg
     2161 atgatgtcgc tatctggcag caggttgtcg aaataggcat tcaccacagc gccacggtgt
     2221 gcggcattgt tgggcaaaaa cggcagtgac agcgagaggg gccgtgtttg cgggtgattg
     2281 agccagcttg cgtggtaggc aaagatttct tcaccgtgag cgagtgtcca ttcccccacc
     2341 agcaggccat tcatccacac attcagctgt tgtcggtggc ttttgcggcc catttcacca
     2401 tgcctcgtcg ctgactgcgg tgggttggtc atcacgggtg tgaatcacca gctcggcatt
     2461 cagcacccgt aagagcttgc tcaaccgcgc tacgctggtg gcggcaggcc tggcttccag
     2521 tcgcgcatag ctttgttggc tgatgcccag ccgttcggcc agctgcgcct gggtcagccc
     2581 ggcgcgctgg cgcaggctgc gcagcatggc gggcaattgt tctggcatga ggatggggta
     2641 gtgcatgctg tgtgctttcg cattgaacac acctaacatc ctatgtgttg taaacgccag
     2701 atacaacata aaagctgtat tttgtattta caacatatag attgtaaaaa tgaggatcat
     2761 gctatccagt ctggctaggc gagcgcagac agtgcaagca gtacggcaag gccgcgcaac
     2821 aacgccagga gagattgctg agcgacgctc agccgccttc cgccagggtg gcgtgaaaca
     2881 gttggttcag cgggtcgagc cgggttgcgc cgccaggggg ccagggcagt tcgggcaggg
     2941 tgcgggccag ttcggtttcg ataaaggcgt tcaggctggg cacggcggcg gcgcggccca
     3001 gctcgggggc ttggcgcttt tgcgccagca gggtgtggat gtcggcgcgc acctgggctt
     3061 cgtcggcgtt cagcatggct aacaggcgct caaattccat cggcggcgcg ccgcgcccgt
     3121 cggcaatcca gcgcgccgcc agcaaggggc gcagcacgta gaagtatttt ttcagcggca
     3181 cttcgtcttg cttgaggtag ccgcgatagt ttttgctggc catgctgcgg tagtgataca
     3241 gcccccgttc cgaggtgtaa atcgcgggca gcagcgccag tgcctgctgg cgaaaatcgc
     3301 cgtgttcgca gtaggtgatg ggggattgca gccattcaat cagcgcgggg ttggatttcc
     3361 acagcaggcg cagcgccttg cgcacatccc agccgttcag gtcgatgtcg tccacaatcg
     3421 ggtattcaat cacatcgcgg cgttcttcca ggtctaccgc cagataccag tcgcgcgggt
     3481 gggcgtagac aaagcgcaca tcgtaatcgc tattgggcga ggcaaagccc caggcgcggc
     3541 tgccggattc aatggcaaac agaatgcgca cctggtgctc ttgttcggcg cgctgcaagc
     3601 ggtgcaggat gtcttggcgg atatcagcgg gaatcatggc atggggccgg cgcaggtcag
     3661 gaaaagccca ctctacccgg cgccaggctg gcattcaagc cagggccatt gcataaacga
     3721 ttggccggcc ctgtgtgcgg cgc
//
LOCUS       DAOPYX010000295         3734 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_32730_length_3734_cov_7.254961, whole genome shotgun sequence.
ACCESSION   DAOPYX010000295 DAOPYX010000000
VERSION     DAOPYX010000295.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 3734)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3734)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3734
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32730_length_3734_cov_7.254961"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1690
                     /locus_tag="PKN55_12350"
     CDS             <1..1690
                     /locus_tag="PKN55_12350"
                     /inference="COORDINATES: protein motif:HMM:NF020137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4214 domain-containing protein"
                     /protein_id="HNX72083.1"
                     /translation="PTLAKSLDTRQYTLMSYTQPANSVYPSAGYVDGKYTWLSYYVNP
                     ETPMVLDIAAMQYLYGANTQYNSGDTTYTFDPSKPFFKTIWDGGGADTISLSNYTLNN
                     IIDLTPGSYSTLRIAPPSNTGGATTTYDGSNALGIAYGAIIENAIGGSGDDTLIGNSA
                     NNQLTGGKGNDTAVFHGNFADYTISYQAGVFTVTDKTSNRDGADTLSSIETLKFADQS
                     KSASQLMAASSDSIAPTLSQGSPANQASSAAPAGDIVLTFSETIARGSGLITLKNAAG
                     TTVASFDAASSTQLSLSGSRLVIDPSAELDYGAQYTLSLAAGSVLDTAGNAYAGLSDY
                     RFTTQSRPSQGQSQTGAASADTLTGTAAADTLQGLAGNDTLSGLGGSDTLDGGDGRDT
                     VLYQAASSGYQITRGQHTTVSYNGGADVLSNVERIQFSDKTLALDISGNAGQAYRVYQ
                     AAFDRTPDNGGLKYWLSVLDGGAGLDTVASGFLGSQEFKTLYGSAPSHADYVSRLYSN
                     VLHRTPDQDGYDYWVNLLNSGQISNVVALVQFSESAENQLMVLGAIQDGISLYL"
     gene            1841..2191
                     /locus_tag="PKN55_12355"
     CDS             1841..2191
                     /locus_tag="PKN55_12355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX72084.1"
                     /translation="MQSIRHGLAQGQRLGWQGQAGQCVEVRRGRVEVEEILWVESQLL
                     CVRQRLGPGSCYVLHSSGWVSVQAVSAAELSLPAPLVDCAAWRRLGRWLTGGWRGLCA
                     EWMGAGRGWRAHGR"
     gene            complement(2392..>3734)
                     /gene="ilvD"
                     /locus_tag="PKN55_12360"
     CDS             complement(2392..>3734)
                     /gene="ilvD"
                     /locus_tag="PKN55_12360"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004285459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HNX72085.1"
                     /translation="NDKVSDEEVAAVERSACPTCGSCSGMFTANSMNCLTEALGLSLP
                     GNGSLVATHADRKELFLQAGRTIVELAKRYYEQDDASVLPRSIATFEAFENAMSLDVA
                     MGGSTNTVLHLLAAAREAGVDFTMQDIDRVSRRVPCLSKVAPATQKYHMEDVHRAGGV
                     IGILGELDRAGLIHRDIPTVHAATLGEGLDRWDIQRPSCDDAAKTLYRAAPGGVRTTI
                     AFSQSMRYPELDADRAEGCIRDKANAYSQDGGLAVLYGNIAERGCIVKTAGVDESILT
                     FTGRVRIFESQDAAVEAILADQIVAGDVVLIRYEGPKGGPGMQEMLYPTSYLKSKGLG
                     KACALLTDGRFSGGTSGLSIGHVSPEAAEGGLIGLAAEGDTLEINIPARTIHLAVSDE
                     ELAQRRAAMLARGADAWKPLNRDRVVSAALRAYAAMTTSADTGAVRDVSQIERR"
BASE COUNT          664 a         1210 c         1235 g          625 t
ORIGIN      
        1 gcccaccctg gcaaaaagcc tggacacccg ccaatacacg ctgatgtcct acacccagcc
       61 cgccaacagc gtatatccct cggccggcta tgtggatggc aaatacacct ggctaagcta
      121 ctacgtcaac ccggaaaccc cgatggtgct ggacatcgcc gccatgcaat acctgtacgg
      181 cgccaacacc caatacaaca gcggcgacac cacctacacc ttcgacccga gcaagccctt
      241 tttcaaaacc atctgggacg gcggtggcgc cgacacgatc tcactcagca actacacgct
      301 gaataacatc atcgacctca cccccggcag ttactccacc ctgcgcattg ccccgcccag
      361 caataccggc ggcgccacca ccacctacga cggcagcaac gccctgggca ttgcctacgg
      421 cgccatcatc gaaaacgcca tcggcggcag cggcgacgac accctgattg gcaacagcgc
      481 caacaaccag ctgactggcg gcaagggcaa cgacaccgcc gtatttcacg gcaactttgc
      541 cgattacacc atcagctatc aggccggcgt attcaccgtt accgacaaaa ccagcaaccg
      601 cgacggcgcc gacaccctca gcagcatcga aaccctgaaa tttgccgacc aaagcaaaag
      661 cgccagccaa ctgatggccg ccagcagcga cagcatcgcc cccaccctca gccagggcag
      721 cccggccaac caagccagca gcgccgcccc ggccggcgat attgtgctca ccttcagcga
      781 aaccatcgcc cgtggcagcg gcctgattac cctgaaaaac gccgccggca ccaccgtcgc
      841 cagctttgac gccgccagca gcacccagct cagcctgtct ggcagccggc tggtgatcga
      901 ccccagcgcc gaactggact acggcgcgca atacaccctc agcctggccg ccggcagcgt
      961 gctggacacc gccggcaacg cctacgccgg cctgagcgac taccgcttta ccacccaaag
     1021 ccgccccagc cagggccaaa gccaaaccgg cgccgccagc gccgacaccc tcaccggcac
     1081 cgccgccgcc gacaccctgc aaggcctggc cggcaacgac accctcagcg gcctgggcgg
     1141 cagcgacacc ctggacggcg gcgacggccg cgacaccgtg ctgtatcaag ccgccagcag
     1201 cggctatcaa atcacccgtg gccaacacac caccgtcagc tacaacggcg gcgccgacgt
     1261 gctcagcaat gtcgaacgca tccagttcag cgacaaaacc ctggccctgg acatcagcgg
     1321 caacgccggc caagcctacc gcgtttacca agccgccttc gaccgcaccc cggacaacgg
     1381 cggcctgaaa tactggctga gcgtgctgga cggcggcgcc ggcctggaca ccgtcgccag
     1441 cggatttttg ggcagccagg aattcaaaac cctgtacggc agcgccccca gccacgcaga
     1501 ttacgtctcg cgcctgtaca gcaatgtgct gcaccgcacc cccgaccagg acggctacga
     1561 ctactgggta aacctgctca acagtgggca aatcagcaat gtggtggcgc tggtgcagtt
     1621 ctccgaatct gccgaaaacc aactgatggt gctgggcgcc atccaggatg gcatctcgct
     1681 gtatctatag gtatattcag ataacagatt ctttttttct ctaccgatac agccggccaa
     1741 aaagccggct gtatcgctgc atggcgcggg gttttatgcc tgtttgccgg gtgggcggct
     1801 ggataagctg tctacatcgt gtgttcgcca ggagaaaagc atgcaatcga ttcgtcatgg
     1861 gttggcgcaa gggcagcgtt tggggtggca ggggcaggcg gggcagtgtg tggaggtgcg
     1921 tcgcggccgg gtggaggtgg aagaaattct gtgggtggag tcgcagttgc tgtgcgtgcg
     1981 gcagcggctg gggccgggca gttgttatgt gctgcatagc agcggttggg tgagcgtgca
     2041 ggcggtcagt gcggcggagt tgagtttgcc ggcgccgctg gtggattgcg ctgcctggcg
     2101 gcgtttgggc cgttggctga caggggggtg gcgtgggctg tgtgcggagt ggatgggcgc
     2161 ggggcgtggc tggcgggcgc atgggcgctg aggtttggat gtggccgtgg tggcgggtgc
     2221 agagcaagcg ccgctcacca aacccagctg gtgcccgacg cagacagcac gggccagtag
     2281 tacggtaagg cgggtagggt gagccgcgtt cagggcgggg cagcgtgtgc tgtcccgccc
     2341 tttttattgc gtatcgtcac tcacccattc ccggctgccc gcgcgggtgg atcagcggcg
     2401 ttcgatctgg ctgacgtcgc gcacggcgcc ggtgtcggcg ctggtggtca tggcggcgta
     2461 ggcgcgcagg gcggcgctga ccacgcggtc gcggttcagc ggtttccagg cgtcggcgcc
     2521 gcgcgccagc atggcggcgc ggcgttgcgc cagttcttca tcgctgaccg ccaggtgaat
     2581 ggtgcgcgcc gggatgttga tttccagggt gtcgccttcg gcggccaggc cgatcaggcc
     2641 gccttcggcg gcttccgggc tgacgtggcc gatggacagg ccggaggtgc cgccggagaa
     2701 gcggccgtcg gtcagcagcg cgcaggcttt gcccaggccc ttggatttca ggtagctggt
     2761 ggggtagagc atttcttgca tgccggggcc gcccttgggg ccttcgtagc ggatcagcac
     2821 cacgtcgccg gccacgatct ggtcggccag gatggcttcg acggcggcgt cctggctttc
     2881 aaagatgcgc acgcggccgg taaaggtgag gatggattcg tccacgccag cggttttgac
     2941 gatgcagccg cgctcggcga tattgccgta cagcacggcc aggccgccgt cttgcgagta
     3001 ggcgttggct ttgtcgcgga tgcagccttc ggcgcggtcc gcgtccagct cggggtagcg
     3061 catgctctgg ctgaaggcga tggtggtgcg cacgccgccg ggggcggcgc gatacagggt
     3121 tttggcggcg tcgtcgcagc tcgggcgctg gatgtcccag cggtccagcc cttcgcccag
     3181 ggtggcggcg tgcacggtgg gaatgtcacg gtggatcagc ccggcgcggt ccagctcgcc
     3241 caggatgcca atcacgccgc cggcgcggtg cacgtcttcc atgtggtatt tctgggtggc
     3301 cggggccact ttggacaggc agggcacgcg gcgggagacg cggtcgatgt cctgcatggt
     3361 gaagtccacg ccggcttcgc gggcggcggc cagcaggtgc agcacggtgt tggtggagcc
     3421 gcccatggcc acgtccaggc tcatggcgtt ttcaaaggct tcgaaggtgg cgatgctgcg
     3481 cggcagcacg ctggcgtcgt cttgttcgta gtagcgcttg gccagttcga caatagtgcg
     3541 gccggcttgc aggaacagtt ctttgcggtc ggcgtgggtg gccaccagcg agccattgcc
     3601 cggcagcgac aggcccagcg cttcggtcag gcagttcatc gagttggcgg tgaacatgcc
     3661 ggaacaggag ccgcaagtgg ggcaggccga gcgttcgacg gcggccactt cttcatcgct
     3721 gaccttgtcg ttgg
//
LOCUS       DAOPYX010000296         3717 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_32982_length_3717_cov_11.858547, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000296 DAOPYX010000000
VERSION     DAOPYX010000296.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 3717)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3717)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3717
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32982_length_3717_cov_11.858547"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..413)
                     /locus_tag="PKN55_12365"
     CDS             complement(<1..413)
                     /locus_tag="PKN55_12365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018149693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNX72086.1"
                     /translation="MVALRVVPVTDEAGQIIEPALLAAAGEVHRQLRPMLPEDYPAKM
                     ARVFAGGARMVVAVHDGRVLGLALYRLYENTYENLRLYVDDLVTDQATRSQGVGGALL
                     DWCETLARSRGAEWLALDSGTWRTDAHRLYFRKGMV"
     gene            complement(417..836)
                     /locus_tag="PKN55_12370"
     CDS             complement(417..836)
                     /locus_tag="PKN55_12370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014088343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HNX72087.1"
                     /translation="MPTVYFEDLSVGQSAEYAKTVTEADILLFSAVSGDNNPVHLNAE
                     YAENSMFKSRIAHGMLSAGFISAVLGTRLPGEGAIYLGQTLKFKAPVRIGDTVTATAT
                     VAALDPAKKRVTLTTVCKVKDTVVIEGEAVVIAPSKA"
     gene            1066..1815
                     /locus_tag="PKN55_12375"
     CDS             1066..1815
                     /locus_tag="PKN55_12375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HNX72088.1"
                     /translation="MKVLVAVKRVVDYNVKVRVKADGSDVDVANVKMSMNPFDEIAVE
                     EAVRLKEAGKATEIVAVSMGVKQCEETLRTALAMGADRAIHVETDAELQPLAVAKLLK
                     QVAAKEAPQLVILGKQAIDDDANQTGQMTAALLGWAQGTFASKVELGDGSVNVTREVD
                     GGLETVKLSLPAVLTTDLRLNEPRYVKLPNIMAAKKKPLDKTSPADLGVDVTPRLKTL
                     KVAEPAKRSAGVKVANAAELVAKLKNEAKVL"
     gene            1820..2758
                     /locus_tag="PKN55_12380"
     CDS             1820..2758
                     /locus_tag="PKN55_12380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008952487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HNX72089.1"
                     /translation="MAILVIAEHDNSQIKSGTLNTVTAAQKLGSDVHLLVAGAGCAAA
                     ADAAKQIAGVSKVLLADAAALAHPLAENLTPLVVDLAKQYSHVLAPATTFGKNLLPRV
                     AALLDVAQISEITAVESADTFVRPVYAGNVMATVQSSDAIKVITVRTTAFEAAAVSGG
                     AAAVEAVSGAADAGLSSFVSQELTVSDRPELAAAKIVVSGGRALGSAEQFKAVIEPLA
                     DKLGAAVGASRAAVDAGYAPNDYQVGQTGKVVAPQLYVAVGISGAIQHLAGMKDSKVI
                     VAINKDEEAPIFQVADYGIVGDLFSVLPELMTELSK"
     gene            2948..>3717
                     /locus_tag="PKN55_12385"
     CDS             2948..>3717
                     /locus_tag="PKN55_12385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008952488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNX72090.1"
                     /translation="MTYQVPLKDIRFVLSELAELPAVCTLPGYEDCNVELVDAILDEA
                     AKFAEGVLAPINRNGDKGNTWKDYQVTTAEGFADAYHQFRDSGWVGLRAPQDVGGQGL
                     PALVAMATEEMWCSANLSFSLAPLLTLGAIEAIHHHASEDLKATYLPRMASGEWTGTM
                     NLTEPNAGSDLAQVRTKAVDNGDGTYSITGQKIFITWGEHDMADNIVHLVLARLPDAP
                     AGVKGISLFIVPKYLVNADGTLGARNDARCVSIEHKLG"
BASE COUNT          736 a         1205 c         1149 g          627 t
ORIGIN      
        1 accatcccct tgcggaaata cagccggtgg gcgtcggtgc gccaggtgcc agaatccagc
       61 gccagccact cggcgccgcg cgagcgggcc agggtttcgc accagtccag caaggcgccg
      121 cccacgccct gcgagcgggt ggcttgatcg gtcaccagat cgtccacata cagccgcagg
      181 ttttcgtagg tgttttcgta caggcgatac aaggccaggc ccagcacgcg cccgtcgtgc
      241 acggccacca ccatgcgcgc gccgccggca aacacccgcg ccattttggc cgggtagtct
      301 tcgggcagca tcggccgcag ctggcggtga acctcgccag ctgccgccag cagcgccggc
      361 tcgatgatct gtcccgcttc gtcagtgacc gggactacgc gcagcgccac catgactcag
      421 gccttgctcg gggcgatcac cacggcttcg ccttcgatca ccacggtgtc tttgacttta
      481 cacacggtgg tcagggtcac gcgctttttg gccggatcga gcgcggccac ggtggcggtg
      541 gcggtcacgg tgtcgccaat gcgcaccggg gccttgaact tcagcgtctg gcccaggtag
      601 atggcgcctt cgcccggcag gcgggtgccc aacacggcag aaatgaagcc agccgagagc
      661 atgccgtggg cgatgcggct cttgaacatc gagttttcgg catactcggc gttcagatgc
      721 accgggttgt tgtcgccaga tacggcagaa aacagcagaa tgtccgcttc cgtcacggtt
      781 ttggcgtatt cggcgctctg accaacggag agatcttcga agtaaacagt aggcacgact
      841 gactcctcgg ttatgttgca ctgcaacgtg gaacttgcca ttctgaggtc agccgcgcaa
      901 aaatgcaatt tgccttgcac actgcccact tgccatcagc gagtttgcag agtacattca
      961 aacagtcgtt taaaaatcgg ctgcgcgcgc gcatgcgtgc gtgcgggcgc ccgtgcaggg
     1021 catgttttta gccactcaca ctgacacatc tgcagctgga ggaatatgaa agtcctagtc
     1081 gccgtgaaac gcgttgtgga ttacaacgtg aaagtccgcg ttaaagcgga cgggtctgac
     1141 gttgatgttg ccaacgtcaa gatgtcgatg aacccgtttg acgaaatcgc cgtcgaagaa
     1201 gccgttcgtc tgaaagaagc cggcaaggcc accgaaatcg tcgccgtctc catgggcgtc
     1261 aagcagtgcg aagaaaccct gcgcaccgcg ctggccatgg gcgccgaccg cgccattcac
     1321 gtcgaaaccg acgctgaatt gcagccgctg gccgtggcca agctgctcaa gcaagtggcc
     1381 gccaaagaag ccccgcaact ggtgattctg ggcaagcagg ccattgacga cgacgccaac
     1441 caaaccggcc agatgaccgc cgcgctgctg ggctgggcgc aaggcacgtt tgcctccaag
     1501 gttgagctgg gcgatggctc ggtgaacgtc acccgcgaag tggatggcgg tctggaaacc
     1561 gtcaagctca gcctgccggc cgtgctgacc accgacctgc gcctgaacga gccgcgctac
     1621 gtcaagctgc ccaacatcat ggccgccaag aaaaagccgc tggacaaaac cagcccggcc
     1681 gatctgggtg tggacgtgac cccgcgcctg aaaaccctga aagtcgccga gccggccaag
     1741 cgctccgccg gcgtcaaggt ggccaacgca gccgagctgg tggccaaact gaaaaacgaa
     1801 gcaaaggtgc tgtaatacta tggctatcct ggttatcgcc gaacacgaca acagccagat
     1861 caagtctggc actctgaaca ccgtcaccgc cgcgcaaaag ctgggcagcg atgtccacct
     1921 gctggtggct ggcgctggct gcgccgccgc tgcggacgcc gccaagcaga ttgctggcgt
     1981 gagcaaggtg ctgctggctg acgccgccgc cctggcccac ccgctggccg agaacctcac
     2041 cccgctggtg gtggatctgg ccaagcaata cagccatgtg ctggccccgg ccaccacctt
     2101 tggcaaaaac ctgctgccgc gcgtggctgc cctgctggac gtcgcccaga tcagcgaaat
     2161 caccgctgtg gaaagcgccg acacctttgt gcgcccggtg tacgccggca acgtgatggc
     2221 caccgtgcaa tccagcgacg ccatcaaggt gatcaccgtt cgcaccaccg cctttgaagc
     2281 cgctgctgtc tccggtggcg ccgccgccgt ggaagccgtc tctggcgctg ccgacgctgg
     2341 cctgtccagc tttgtcagcc aggaactcac cgtgtctgac cgcccggaac tggctgccgc
     2401 caagatcgtg gtgtctggtg gccgcgcact gggttcggct gagcaattca aggccgtgat
     2461 cgagccgctg gccgacaagc tgggcgctgc cgtgggcgca tcccgtgccg ctgtggacgc
     2521 cggctacgcg ccgaacgact accaagtggg ccaaaccggc aaagtggtgg cgccgcagct
     2581 gtacgtggcc gtgggcattt ctggcgccat ccagcacctg gccggcatga aggactccaa
     2641 agtcatcgtc gccatcaaca aagacgaaga agccccgatc ttccaggtgg cggattacgg
     2701 cattgtgggc gacctgttca gcgtgctgcc ggaactgatg accgaattgt ccaagtaagc
     2761 aagacgcgcg gatggccctg gccacacagg gccggcgccc agacgccgcc agaagcgtcg
     2821 gtcgccccgc ctgcccggac aacccggcgg gtgaatccac acggctgtca ccagagtaga
     2881 gtcagagcgc ccgtctggcg gagagaactc tgagtggtgg ccgtttctac atcgcagagg
     2941 agtaagcatg acctatcaag tcccgctcaa ggacatccgt tttgtgctgt ctgaactggc
     3001 cgagctgccg gcggtgtgca ccctgcccgg ctacgaagat tgcaatgtcg aactggtgga
     3061 cgccattctg gacgaagccg ccaagtttgc cgaaggggtg ctcgccccga tcaaccgcaa
     3121 cggcgacaag ggcaacacct ggaaagacta ccaggtgacc accgccgaag gctttgccga
     3181 cgcctaccac cagttccgcg acagcggctg ggtgggcctg cgcgccccgc aagacgtggg
     3241 cggccagggc ctgccggcgc tggtggcgat ggccaccgaa gaaatgtggt gttccgccaa
     3301 tctgtcgttt tcgctggccc cgctgctgac cctgggcgcc atcgaagcca tccatcacca
     3361 cgccagcgaa gacctgaaag ccacctacct gccgcgcatg gccagcggcg agtggaccgg
     3421 caccatgaac ctgaccgaac ccaacgccgg ctctgatctg gcgcaggtgc gcaccaaggc
     3481 cgtggacaat ggcgatggca cgtattccat caccggccaa aagattttca tcacctgggg
     3541 tgagcacgac atggccgata atatcgtcca tctggtgctg gcccgtctgc cggacgcccc
     3601 ggcgggggtg aagggcattt cgctgtttat cgtgcccaaa tacctggtca acgccgacgg
     3661 cacgctgggc gcgcgcaacg acgcccgctg cgtgtccatc gaacacaagc tgggcat
//
LOCUS       DAOPYX010000297         3677 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_33494_length_3677_cov_10.108504, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000297 DAOPYX010000000
VERSION     DAOPYX010000297.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 3677)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3677)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3677
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33494_length_3677_cov_10.108504"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            241..732
                     /locus_tag="PKN55_12390"
     CDS             241..732
                     /locus_tag="PKN55_12390"
                     /inference="COORDINATES: protein motif:HMM:NF022517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HNX72091.1"
                     /translation="MFATFAVDSCLNGQAADYPYRDHFHTSCPGQPPDAQALLQAFVD
                     APPAWAQALMRARDRLMAPLGLKTADLPRQAPQARVGEAVGVFCVWHAGPDELLLGQR
                     DRHLDFCLWLGVRAGRLHLYTGVRPHHLGGWLYLALVWPFHWLIVRRSLARMAAHCSE
                     AAC"
     gene            726..2198
                     /locus_tag="PKN55_12395"
     CDS             726..2198
                     /locus_tag="PKN55_12395"
                     /inference="COORDINATES: protein motif:HMM:NF013584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HNX72092.1"
                     /translation="MLSATAALRALALGDGTASQLLEAHVARIAQHNPRIRALVTLDI
                     ERARAAAQAVDALPLAERARRLAGEQPLLGLPISIKDAFATQGLRTTSSHPPLANYLP
                     AADATLVARLKAAGAIVLGKSNLSELAGDPQCWSPLFGPTHNPWELSLTPGGSSGGSA
                     AAIAMGFAALELGSDIGGSIRIPAACCGVVGFKASENRLPRTGHIPHLPGQPRSVRHM
                     LSFGLLARTVADVAHAMPSLAGADGEDQEVPPLPWRPQPPLTRPLRLAWCDDFAGLPL
                     CPRTRAGLTQALAGARAAGHQLLRRQPAGPPLAQVWRAFGVLAGSEIGLALPARQRWP
                     LALLAGLLPASQPIAQGFAQGARLSLRRLQWALGVRDAWLRALESMLYEVDAWLCPTL
                     LCTAWPASPPPRHGGLPAPLNIGGRRVPYLEATLSLTSLFSLSGSPVLSLPVGVLDGV
                     PVGVQLVGRKWRDEALLAMGESLTAAWPAMPMPPGAQVDD"
     gene            2449..>3677
                     /locus_tag="PKN55_12400"
     CDS             2449..>3677
                     /locus_tag="PKN55_12400"
                     /inference="COORDINATES: protein motif:HMM:NF024140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNX72093.1"
                     /translation="MSLFSRLTLAWRLTLGFGLVLALMVGITLTGIQKVNFIDRSLQQ
                     VTDVNAVKQRYAINFRGSVHDRAIVMRDVALETDPARHPPLLSEITRLEKFYQESAVE
                     LARAIARDGDPEEQRRWQAIQAIEQRTQPLVKQALAHLEAGRHDDTRAVLMGDLRPAF
                     TDWLAAINALIDYEEQKSQQVTPLARAVASQFQTLMLALCGVSLVIGALVALGITRQL
                     LRSLGGEPAEAAAQVTQMASGDLRASIRARYPDSMLAAVAHMQTRLKTLLTGMLDSSG
                     ELDQRARHVTQAAQTAQQAAQRQSDASAGAAASIEQLTQSIEQVAQIARQTEHNSGQT
                     AELSENGRQAVRTAADEIGRIAQAVQGSSAHMRALRQRSQDIGSIASVIGEIADQTNL
                     LALNAAIEAARAGETGRG"
BASE COUNT          564 a         1304 c         1227 g          582 t
ORIGIN      
        1 ttcgctacaa gatcagcgcc cggcctcagc gctaacctat gccctgacag ccgcttaaag
       61 cggctaacaa aaccctcctg gcgtcgttgc gcagccttgc cgtactactt cgcttacaac
      121 accctcctgg cgtgattgcg cgtgtgcggc cacaggcccg gcgcggtgtt ttgtgagcga
      181 cgctcgacac gacacaccgc gcgcgtgtcg ccatgccctt actttgcccg gagctttgcc
      241 atgtttgcca cgtttgcggt ggattcctgc ctgaatggac aggcggccga ttatccgtat
      301 cgggatcact ttcacacctc ttgccctggc cagccgccgg acgcgcaggc gctgttgcaa
      361 gcctttgttg acgcaccgcc ggcctgggcg caggcgctga tgcgcgcgcg cgaccggctg
      421 atggccccgc tgggcctgaa aaccgcagat ttaccccgcc aagcgccgca ggcgcgcgtg
      481 ggcgaggcgg tgggggtgtt ttgcgtgtgg catgccgggc cggacgagct gctgctgggc
      541 cagcgcgacc gccatctgga tttttgcctg tggctgggcg tgcgcgccgg gcgcttgcat
      601 ctgtataccg gcgtgcgccc gcatcacctg ggcggctggc tgtatctggc gctggtgtgg
      661 ccgtttcact ggctgattgt gcgccgcagc ctggcgcgca tggccgcaca ctgctcggag
      721 gcggcatgct gagcgccacc gccgcgctgc gcgcgctggc gctgggcgat ggcacggcca
      781 gccaactgct ggaggcgcat gtggcgcgca ttgcccagca caacccgcgc atccgcgcgc
      841 tggtgacgct ggacatcgaa cgcgcccgcg ccgccgcaca ggcggtggat gcgctgccat
      901 tggccgagcg cgcgcgccgg ctggcgggcg aacagccgct gctgggcctg ccaatcagca
      961 tcaaggacgc ctttgccacc cagggcttgc gcaccaccag cagccacccg ccgctggcga
     1021 attatctgcc cgccgccgac gccaccctgg tggcgcggct gaaggccgcc ggggcgattg
     1081 tgctgggcaa gagcaatttg tctgaactgg ccggcgaccc gcaatgctgg agtccgctgt
     1141 ttggccccac ccataaccct tgggagctga gcctgacgcc gggcggcagt tccggcggca
     1201 gcgcggcggc catcgccatg ggttttgccg cgctggagct gggcagcgat attggcggct
     1261 ccatccgcat tccggccgcc tgctgcggcg tggtgggctt caaggccagc gaaaaccgcc
     1321 tgccgcgcac cgggcatatt ccgcacctgc ccggccagcc gcgcagcgtg cgccatatgc
     1381 tgtcgtttgg cctgctggcg cgcacggtgg ccgacgtggc gcacgccatg cccagcctgg
     1441 ccggcgccga tggcgaggat caggaagtcc cgccgctgcc gtggcggccg cagcccccgc
     1501 tgacgcggcc cttgcggctg gcctggtgcg atgattttgc cggcctgccg ctgtgtccgc
     1561 gcacccgcgc cgggctcacg caggcgctgg ccggcgcgcg cgccgccggc catcaactgc
     1621 tgcgccgcca gccggccggc ccgccgctgg cgcaggtgtg gcgggcgttt ggcgtgctgg
     1681 ccggcagcga aatcggcctg gccctgcccg cccgacaacg ctggccgctg gcgctgctgg
     1741 ctgggttgct gccggccagc cagccgattg cgcaaggctt tgcccaaggc gcgcggctgt
     1801 cgctgcgccg gctgcaatgg gcgctgggcg tgcgcgacgc ctggctgcgc gcgctggaaa
     1861 gcatgttgta cgaggtggac gcctggcttt gccccaccct gctgtgcacc gcctggccag
     1921 ccagcccgcc gccgcgccat ggcggcctgc cggcgccgct gaatatcggc gggcggcgcg
     1981 tgccctatct ggaggccacg ctcagcctga ccagcctgtt ttcgttgtcg ggcagcccgg
     2041 tgctgagcct gccggtgggg gtattggatg gcgtgccggt gggcgtgcaa ttggtggggc
     2101 gcaaatggcg cgacgaggcc ctgctggcga tgggcgagtc actgaccgcc gcctggccgg
     2161 cgatgccaat gccgccgggg gcgcaggtgg acgactgaga cgctcagccc ccgcacacca
     2221 cgacgctacg cgggggcggg ccgacaacgc ggacggcgac tgtatcagcc cgctgcctgc
     2281 ttatgagggg ctcgattgtg tttatctatg ataataagat aaacaattaa ttttccctgt
     2341 ggcagacaaa ccggcatact ggaaccgatt acccttcctc cggtcatctc cccggtgtcc
     2401 gcgccatcat ggcgcgccga ccgcactgtg catcccaagg agcatgtcat gtcactgttt
     2461 tcccgactca ccctcgcctg gcggctgacg ctgggctttg gcctggtgct ggccctgatg
     2521 gtgggcatca ccctgaccgg catccagaag gtcaatttca tcgaccgcag cttgcagcaa
     2581 gtcaccgacg tcaacgccgt caaacagcgc tacgccatca actttcgcgg cagcgtacac
     2641 gaccgcgcca tcgtcatgcg cgacgtggcg ctggaaaccg acccggcccg ccacccgccc
     2701 ttgctgagcg aaatcacccg gctggagaag ttttaccagg aatccgccgt cgagctggcg
     2761 cgcgccatcg cccgtgacgg cgacccggaa gaacagcgcc gctggcaagc catccaggcc
     2821 atcgagcaac gcacccagcc cttggtcaag caggcgctgg cccacctgga agccggccgc
     2881 catgacgaca cccgcgccgt gctgatgggg gatttgcgcc cggccttcac cgactggctg
     2941 gcggcgatca atgcgctgat tgattacgaa gaacaaaaaa gccagcaagt caccccgctg
     3001 gcgcgcgccg tggccagcca gtttcaaacc ctgatgctgg cgctgtgcgg cgtcagcctg
     3061 gtgattggcg cgctggtggc gctgggcatc acccgccagc tgctgcgcag cctgggcggt
     3121 gagccggccg aagccgccgc gcaagtcacc cagatggcca gcggcgacct gcgcgccagc
     3181 atccgcgccc gctacccgga cagcatgctg gcggcggtgg cgcatatgca aacccggctg
     3241 aaaaccctgc tcaccggcat gctggattcg tccggcgaac tggaccagcg cgcccgccac
     3301 gtcacccagg ccgcgcaaac cgcgcaacaa gccgcccagc gccaatccga cgccagcgcc
     3361 ggcgcggcag ccagcattga gcaactgacg caaagcatcg agcaagtggc gcaaattgcc
     3421 cgccagaccg aacacaactc cggccagacc gccgagctct cggaaaacgg ccgtcaggca
     3481 gtgcgcaccg ccgccgacga aatcggccgc atcgcccagg cggtgcaagg ctcgtcggcg
     3541 catatgcgcg cgctgcgcca gcgctcgcaa gacatcggca gcatcgccag cgtgattggt
     3601 gaaatcgccg accagaccaa tctcttggcg ctgaacgccg ccatcgaagc cgcccgcgcc
     3661 ggcgaaaccg gccgggg
//
LOCUS       DAOPYX010000298         3672 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_33556_length_3672_cov_9.567321, whole genome shotgun sequence.
ACCESSION   DAOPYX010000298 DAOPYX010000000
VERSION     DAOPYX010000298.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 3672)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3672)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3672
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33556_length_3672_cov_9.567321"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(67..354)
                     /locus_tag="PKN55_12405"
                     /pseudo
     CDS             complement(67..354)
                     /locus_tag="PKN55_12405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216212.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3579 domain-containing protein"
     gene            complement(538..2670)
                     /gene="dinG"
                     /locus_tag="PKN55_12410"
     CDS             complement(538..2670)
                     /gene="dinG"
                     /locus_tag="PKN55_12410"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008953624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HNX72094.1"
                     /translation="MLTDAEKDAIRHHYQAIGKSLPGFRPRAAQRQMLASIAQAFARS
                     KPREGDALPERNGESIVVVEGPTGVGKSLAYLLAGCVMAKSRGKKLVISSATIALQEQ
                     LVNRDLPFVLSHSGLEASFALAKGRGRYLCPYRLYQHTADASQGELLAPDPNMLLWNH
                     KPEKRELEQLKRMADAFYYRRWDGDRDAFDETVEDRLWSRVTNDRHGCLKSACPNRSE
                     CPFYVARDQLDQVDIVVSNHDLLLADASMGGGVILPPPIDTFYCIDEAHQLAKKAINQ
                     FAADHQVQQALWWLDKLDATVGRAEALISRKELATQALDAATGCAQGLGELAQLLTPL
                     AQLEPGADEPEPTWLLENGELPENMALTAANLNVSAATLLKQLTAVQDALVEARRDKN
                     EDSGQIDQLGSELGFFIARAEALAAVWALMCATPPEGAPPIAKWITTRQPGSGRRDWQ
                     VCASPVSAAADLANNLWRLAAGAVLTSATLRSLGSFDLLLAQTGLKWLGDTECVALES
                     PFDFQRQGELYVPAMRASPKDAPAHTAEIIQLLPKLIDAAAPLGTLVLFTSRRQMQEV
                     HDGLPAELAACVLVQGSLPKAVLLARHHERLAAQQASVIFGLDSFAEGLDLPGEACVQ
                     VILAKLPFAMPDDPVGQTLSQWIESRGGNPFIEITVPEASIKLIQAVGRLIRTETDYG
                     RVTILDNRLLRQAYGKKLLAALPPFRRL"
     gene            2777..3550
                     /locus_tag="PKN55_12415"
     CDS             2777..3550
                     /locus_tag="PKN55_12415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011135893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HNX72095.1"
                     /translation="MMLPDTADPHDLSLPILYQDEHLVAVHKPSGLLVHRSPIDRQET
                     RFALQTVRNQLGRHVYGVHRLDKGTSGILLFALSPEAGRTLSQAFEAQQVDKRYLAVV
                     RGWPDAQGEIDYPLSRQPDEREPGPVTPAPPQSAHSSYQRLATVELPVAVERYPSSRY
                     ALLALTPHTGRRHQLRRHLKHISHPIIGDATHGRGRHNRMFAERFAVNRLLLAATGLR
                     LPHPVSGETLTLHCPPSAEFLHVLTELGWGDAWQDASRL"
BASE COUNT          605 a         1199 c         1190 g          678 t
ORIGIN      
        1 gcccccggcg tattccccgc gccggcggca tgggctggct cagcccaaaa actggttcgg
       61 gtagacttcg tcgatcagcg tgcggcagtc caccacgcgc aggtcgttgt ccttggcgaa
      121 ggacagcaga aactggtaga cgcgagggtc gattttttcc agtttcttgt ccaccgccac
      181 gcagcggaca ttgtccatca tcagcggacg gacatagctg tggtaagaca ttttcttgtc
      241 ggcgccaaaa ctggccagga tgccggacaa gcgctcggcc cagtcgctgg ggcggaaagt
      301 gcgcccctcg gacgtgatgc cttgaataat gatttcgtaa ggattacaga tcatgttgcc
      361 agaacgccag gtgtgtctct cgcggagagg gaggggtgga tgagcatccg ccagctgatg
      421 ccagtcggtg cacatcgcgt ttttttcgat tctacgcctt tttcgttcgc tcttgaatca
      481 cgctacggca gtttgcgtga aaaaagcgtc cgctgtggcg gattgcgcca attggcgcta
      541 caagcggcga aagggcggca gcgcggccag cagtttcttg ccgtaagcct ggcgcaacag
      601 gcgattatcc aggatggtca cccggccgta gtcggtttcg gtgcggatca gccggcccac
      661 ggcctgaatc agcttgatcg acgcttctgg cacggtgatt tcgatgaacg ggttgccgcc
      721 gcgcgattca atccactgcg acagcgtttg ccccaccggg tcgtccggca tggcgaacgg
      781 cagcttggcc agaatcacct gcacgcaggc ttcaccgggc aagtccagcc cttcggcaaa
      841 tgaatccaga ccaaaaatca cgctggcctg ctgggcggcc aggcgctcgt ggtggcgcgc
      901 cagcagcact gccttgggca ggctgccttg caccagcaca caggcggcca gctcggccgg
      961 caggccgtcg tggacttcct gcatctgcct gcgcgaggtg aacagcacca aagtgcccag
     1021 gggcgcggcg gcgtcgatca gcttgggcaa gagctggatg atttcggcgg tgtgcgctgg
     1081 cgcatccttg ggtgaggcgc gcatggccgg cacgtatagc tcgccctggc gctgaaaatc
     1141 aaacggggat tccagcgcca cgcattcggt gtcgcccagc catttcagcc cggtttgcgc
     1201 cagcagcaaa tcaaacgagc ccagcgagcg cagcgtggcc gaggtcagca ccgcgcctgc
     1261 ggccagccgc cacagattat tggccagatc cgccgccgcc gacacgggcg aggcgcacac
     1321 ctgccagtcg cgccggccgc tgccgggctg gcgggtggtg atccatttgg cgatgggcgg
     1381 cgcgccttct ggcggggtgg cgcacatcag cgcccacacc gccgccagcg cctcggcgcg
     1441 ggcaataaaa aaccccagct cgctgcccag ttggtcgatt tgcccgctgt cttcgttttt
     1501 gtcgcggcgc gcttccacca gcgcatcctg cacggcggtc agttgcttga gcaaggtggc
     1561 ggcggagaca ttcaaattgg cggcggtcag cgccatattc tccggcagct cgccgttttc
     1621 cagcagccag gtgggttcgg gctcgtcggc gccgggctcc agctgggcca gcggggtgag
     1681 cagctgcgcc agctcgccca ggccctgcgc acagccggtg gcggcgtcca gcgcctgggt
     1741 ggccagctct ttgcggctga tcagcgcctc ggcgcggccg acggtggcgt ccagcttgtc
     1801 cagccaccac agcgcctgct gcacctggtg gtcggcggca aattgattga tggctttttt
     1861 ggccagttgg tgggcttcgt cgatgcagta aaacgtgtcg atcggcggcg gcagaatcac
     1921 cccgccgccc atcgaggcgt cggccagcag caaatcatgg ttggacacca cgatgtccac
     1981 ctggtcgagc tggtcgcgcg ccacataaaa cgggcattcg ctgcggttgg ggcaggcgga
     2041 tttcaggcag ccgtggcggt cgttggtcac ccggctccac agccggtctt ccacggtttc
     2101 gtcaaacgca tcgcggtcgc cgtcccagcg ccggtaataa aacgcatcgg ccatgcgctt
     2161 gagctgctcc agctcgcgtt tttctggttt gtgattccac agcagcatat tcgggtcggg
     2221 cgccagcagc tcgccctggc tggcgtcggc ggtgtgctgg tacaggcgat aggggcacag
     2281 atagcgcccg cgccccttgg ccagggcaaa gctggcctcc aggccgctgt ggctcagcac
     2341 aaaaggcaaa tcgcggttga ccagctgctc ttgcagggcg atggtggccg agctgatcac
     2401 cagctttttg ccgcgcgatt tggccatcac acagccggcc agcaaatagg ccaggctctt
     2461 gcccacgccg gtggggcctt ccaccaccac aatgctttcg ccattgcgct caggcagcgc
     2521 atcgccctcg cgcggcttgc tgcgggcaaa cgcctgggca atgctggcca gcatttgccg
     2581 ctgggcggcg cgcggacgaa agcccggcag gcttttgccg atggcttggt aatggtgacg
     2641 gatggcgtct ttttcggcgt cggtgagcat ggcgccattg tagcgcaaag gccggaatgc
     2701 cgggcgggcc agggcgacag caactgttgc ccatggcgca cgcctgctat gctgcgcgtt
     2761 ttgcctgctg cgaccgatga tgctgcctga taccgctgac ccccacgatt tgagcttgcc
     2821 gattttgtat caggacgagc atctggtggc ggtgcacaag ccttccggct tgctggtgca
     2881 ccgctcgccc atcgaccgcc aggaaacccg ctttgccctg caaaccgtgc gcaaccagct
     2941 gggccgccat gtgtatggcg tgcatcggct ggacaagggc acctcgggca tcttgctgtt
     3001 tgccctcagc cccgaggccg ggcgcacgct gtcgcaggcg tttgaggccc agcaggtgga
     3061 caagcgctat ctggccgtgg tgcgcggctg gccggacgcg caaggcgaga ttgattaccc
     3121 gctgtcgcgc caacccgacg agcgcgaacc cggcccggtg acgccagcgc cgccccagtc
     3181 ggcgcacagc agctatcagc ggctggccac ggtggaactg cccgtggcgg tggagcgcta
     3241 ccccagcagc cgctacgccc tgctggcgct gaccccgcac accggccgcc gccaccagct
     3301 gcgccgccac ctcaagcaca tcagccaccc gatcatcggc gacgccaccc acggccgcgg
     3361 ccggcataac cgcatgtttg ccgagcgctt tgccgtcaac cgcctgctgc tggccgccac
     3421 cgggctgcgt ctgccgcacc cggtcagcgg tgaaacactg accttgcatt gcccaccatc
     3481 ggcagagttt ttgcatgtgc tgaccgaact gggctggggc gacgcctggc aggacgccag
     3541 ccgactctga gaagccgctc acaaccccct ctgaccttgc catgtctgcg ggtatgtacc
     3601 tggccaggat cgccacgctg ggcttgatcc gcggcgctca ggattgctgc tcgaccttgg
     3661 cgtattgctt gt
//
LOCUS       DAOPYX010000299         3666 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_33638_length_3666_cov_8.631404, whole genome shotgun sequence.
ACCESSION   DAOPYX010000299 DAOPYX010000000
VERSION     DAOPYX010000299.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 3666)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3666)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3666
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33638_length_3666_cov_8.631404"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1939)
                     /gene="rnr"
                     /locus_tag="PKN55_12420"
     CDS             complement(<1..1939)
                     /gene="rnr"
                     /locus_tag="PKN55_12420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012698035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HNX72096.1"
                     /translation="MVSKQKKQKNFSLREQDPYLAREREKYDHPLPSREFILQQLENE
                     GAPMFPDELAALLDIQTDEMSFFVRRLRAMEREGQVMINRKGAVCVVDKLDLKTGRII
                     GHKDGFGFIKPDDGSADLFVGEREMHKVLHGDRVMFRQVGTDRRGRPEGSIIEVIERV
                     NQSVVARLYCERGVWFAVPEDRRLNQDILIEPDGHADGEHGQVVMVELVEQPSRHKQP
                     MGRVIQVLGNYADAGMEIEIALRKHDLPHEFSEGALEQARHTPKKVRKKDLTADRRDL
                     RDLPLVTIDGETARDFDDAVYCEPQGKGFRLVVAIADVSHYVQPGDALDLTSIERGNS
                     VYFPRRVIPMLPEALSNGICSLNPEVERLCMVCDMNINSRGEIRGYTFYPAVMLSKAR
                     LTYNQVWDWLQHGSEHPMLPHVQNLYALFKVLLASRNRRGAIDFETTETQMLFNEQGK
                     IERIVPVTRNDAHRLIEECMLAANVCSADFLLKHTHPCLYRVHEGPTPEKLEVLHGFL
                     QLAGLTLPGGDAPTAKDYAKLMDKIKPRPDAAVLQTMLLRSLQQAMYSPDNVGHFGLA
                     YEAYTHFTSPIRRYPDLLVHRAIKAILQGGQYTPPQTWAALGENCSMTERRADDATRD
                     VDAWLKCYFMRDKVGEVFTGKI"
     gene            2017..2103
                     /locus_tag="PKN55_12425"
     tRNA            2017..2103
                     /locus_tag="PKN55_12425"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2051..2053,aa:Leu,seq:cag)
     gene            2166..2252
                     /locus_tag="PKN55_12430"
     tRNA            2166..2252
                     /locus_tag="PKN55_12430"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2200..2202,aa:Leu,seq:cag)
     gene            complement(2458..3414)
                     /locus_tag="PKN55_12435"
     CDS             complement(2458..3414)
                     /locus_tag="PKN55_12435"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HNX72097.1"
                     /translation="MSIDSLTLRLGARWPLPVLSPRTLAVAAGFAMLYLSWGSTFLAN
                     HLALQGFPALVLAGLRFLLAGALFAVLAVARAGGARLGWHDARLALIGGVLMLAIGQG
                     AMVYGVQHLPSGLAALVCASLPLWTVILQAVLPGERRPSPLALFGLGVSMVGMAALLG
                     SRMDVLAEPERLGAVAVLALAQLAWACGSFLFRRTGRGYSQLWWGMSAQMLLAGVVLL
                     AAAAICGQWQAFAWREVSTDSVLALAYLVGVVTALTYPVYFWLLKVSTPAKVSTFAFV
                     APVVALTLGCLVLGETVSTAMLGAAALIVAGVVALTLGQRGG"
BASE COUNT          739 a         1174 c         1079 g          674 t
ORIGIN      
        1 tgatcttgcc ggtgaacact tcgcccacct tgtcgcgcat gaagtagcac ttcagccagg
       61 cgtccacatc gcgggtggcg tcgtcggcgc ggcgttcggt catcgaacag ttttcgccca
      121 gcgccgccca ggtttgcggc ggggtgtact ggccgccctg caggatggcc ttgatggcgc
      181 ggtgcaccag caaatccgga taccggcgaa tcggcgaggt gaagtgggtg taagcctcat
      241 acgccaggcc aaagtggccg acgttgtccg ggctgtacat cgcctgttgc aggctgcgca
      301 gcagcatggt ttgcagcacg gcggcgtccg ggcgcggctt gatcttgtcc atcagcttgg
      361 cgtagtcctt ggcggtcggc gcatcgccgc cgggcagggt caggccggcc agttgcagaa
      421 agccgtgcag gacttccagt ttttccgggg tggggccttc gtgcacgcgg tacaggcaag
      481 gatgggtgtg cttgagcagg aaatccgccg agcagacgtt ggcggccagc atgcattctt
      541 cgatcagccg atgggcgtcg ttgcgggtga ccggcacaat gcgctcgatt ttgccctgtt
      601 cgttgaacag catctgcgtt tcggtggttt caaagtcgat ggcgccgcga cggttgcggg
      661 aggccagcag caccttgaac agcgcataca ggttttgcac atgcggcagc atcgggtgct
      721 cgctgccgtg ttgcagccag tcccacacct ggttgtaggt cagccgcgcc ttggacagca
      781 tcaccgccgg gtagaaggtg tagccgcgaa tctcgccacg gctgttgatg ttcatgtcgc
      841 acaccatgca caggcgttcg acttccgggt tcagcgagca aatgccgttg gacagcgcct
      901 cgggcagcat cgggatcacc cgacgcggga aatacaccga attgccgcgc tcgatcgagg
      961 tgaggtccag cgcgtcgccg ggctgcacat aatggctgac gtcggcaatc gccaccacca
     1021 gacggaaacc cttgccctgc ggttcgcaat acaccgcgtc gtcaaagtcg cgcgcggttt
     1081 cgccgtcgat ggtcaccagc ggcaggtcgc gcaggtcgcg gcggtcggcg gtgaggtctt
     1141 ttttgcggac tttcttgggg gtgtggcggg cttgttccag cgcgccttca gagaattcat
     1201 gcggcaggtc gtgcttgcgc agggcgattt cgatttccat gccggcatcg gcgtaattgc
     1261 ccagcacctg aatcactcgg cccatcggct gcttgtggcg gctgggttgt tccaccagct
     1321 cgaccatcac cacctggcca tgctcgccat cggcgtggcc gtcgggctcg atcagaatat
     1381 cctggttcag gcggcggtct tccggcacgg caaaccagac gccgcgctcg caatacagtc
     1441 gcgccaccac cgattgattg acccgctcaa tcacttcaat gatggagcct tctggccggc
     1501 cgcgccgatc cgtgcccacc tggcggaaca tcacccggtc gccgtgcagc actttatgca
     1561 tttcacgctc gccgacaaac aggtcggcgc tgccgtcgtc cggcttgata aaaccaaagc
     1621 cgtccttgtg gccgatgatg cgcccggttt tcagatcaag cttgtcgacc acgcacaccg
     1681 cgcctttgcg gttgatcatc acctgccctt cgcgctccat ggcgcgcaag cggcggacaa
     1741 aaaagctcat ctcgtcggtc tggatgtcca gcagcgcggc cagctcgtcg ggaaacatcg
     1801 gcgcgccttc gttttccagc tgttgcagga tgaactcccg gctgggcagc gggtggtcgt
     1861 atttctcccg ttcacgggcc aaatacgggt cctgctcgcg cagggaaaag tttttttgtt
     1921 ttttttgctt gctaaccatt gacagtctca aaaaagggct tataatgcac aactcattca
     1981 gcgctgcact gaaagcaaaa cagcacagcg cgatgtgccc aggtggcgga attggtagac
     2041 gcactaggtt caggtcctag cggtggcaac actgtggaag ttcgagtctt ctcctgggca
     2101 ccaaaggttt caaaggaaac cagcagtagt ttaactggtt ttcagcaaca atccgagaag
     2161 tttttgccca ggtggcggaa ttggtagacg cactaggttc aggtcctagc ggtggcaaca
     2221 ctgtggaagt tcgagtcttc tcctgggcac catccgcaaa aagcccctca gcactgctga
     2281 ggggcttttt ctttgcctat catcagacaa acgagttatc tcagctcatg gttgtctgcc
     2341 gacgcatgct gtccaccagg tggaatgtca ccccgaaatc catcgcctca gtggccaagc
     2401 aataaaaaaa cggactgcga cgcctgccca gtccgttttt ttgcgttacc ccaaggttta
     2461 tccgccgcgc tggcccagcg tcagcgccac cacccccgcc acaatcaacg ccgccgcgcc
     2521 caacatggcg gtgctgacgg tttcgcccag caccaggcaa cccagcgtca acgccaccac
     2581 cggtgcgaca aaagcaaaag tggatacctt ggccggcgtg ctgactttaa gcagccaaaa
     2641 atacactgga taggtcagcg cagtcaccac tcccaccaga taggccagcg ccagcacgct
     2701 atcggtgctg acttcacgcc aggcaaacgc ctgccactgg ccgcaaatcg ccgccgccgc
     2761 cagcaacacc acgccagcca gcagcatctg cgcactcatg ccccaccaca gctggctgta
     2821 gccacgcccg gtgcggcgaa acaaaaagct gccgcacgcc caggccagct gcgccaaggc
     2881 cagcaccgcc actgcaccca gacgctcggg ctcggccagc acgtccatgc gactgcccaa
     2941 caaggccgcc atgcccacca tgctcacccc caggccaaac agcgccagcg ggctgggccg
     3001 gcgctcgccg ggcagcacgg cttgcaaaat caccgtccac aacggcaggc tggcgcacac
     3061 cagcgccgcc aggcccgaag gcagatgctg cacgccatac accatggcgc cctggccaat
     3121 ggccagcatc agcacgccgc caatcagcgc cagccgggca tcatgccagc ccaggcgcgc
     3181 gccaccggca cgggcaacgg ccagcacggc aaacaaggca ccggccagca aaaagcgcaa
     3241 gccggccagc accagcgccg gaaaaccttg cagcgccaaa tggttggcca gaaatgtgct
     3301 gccccagctc aggtacagca tggcaaagcc ggcagccacc gccaaggtgc gcggcgacaa
     3361 tactggcagc ggccagcgtg cgcccaggcg cagggtcagg ctgtcaatcg acatgcgctc
     3421 aactccaaag tattcagatc atgatgtggg ccatgctagc caagatcatg agcagaacaa
     3481 tttgaattaa gcgtagattg atttgtgtgg gctattaatt ggcgccgctc acagaatgca
     3541 gccaagcggc cctggcgagt cggcagtcac catggccaga aggggtttgt gagccacgct
     3601 tagcccgcag cctgacgctt accctcaacg ccagggcatc accggcaccg acgaaatgct
     3661 attggc
//
LOCUS       DAOPYX010000300         3649 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_33894_length_3649_cov_11.353645, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000300 DAOPYX010000000
VERSION     DAOPYX010000300.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 3649)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3649)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3649
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33894_length_3649_cov_11.353645"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(236..1306)
                     /locus_tag="PKN55_12440"
     CDS             complement(236..1306)
                     /locus_tag="PKN55_12440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012698603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter ATP-binding protein"
                     /protein_id="HNX72098.1"
                     /translation="MSIEIRNLRKTFGEFDVLKDLSLTVESGELLALLGPSGCGKTTL
                     LRIIAGLETADAGQVLFHGEDATQRPVSERQVGFVFQHYALFRHMSVFDNVAFGLSVR
                     PRASRPAKADIRERVMRLLKLVQLDWLADAYPDQLSGGQRQRIALARSLAVEPRVLLL
                     DEPFGALDAKVRKDLRRWLRQLHDDIHLTSVFVTHDQEEALEVSDRVVVMNRGVIEQV
                     GRPEEIYEHPASPFVTQFLGDVNLFHGRVGANHVEIGDYAHPLDGQAPAGKGEASVYI
                     RPHDLEISREAGQALATGTIEHIHSVGPIVRVELRRQGSGELIDAALTRDDYQQLRLA
                     LGDTVYVRPRKLAVFLDEGANI"
     gene            complement(1340..2296)
                     /gene="cysW"
                     /locus_tag="PKN55_12445"
     CDS             complement(1340..2296)
                     /gene="cysW"
                     /locus_tag="PKN55_12445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012698602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysW"
                     /protein_id="HNX72099.1"
                     /translation="MNLSSATDRRSRSTTEPAWVRLTLTALALGYLAVFLLTPLVSVF
                     YEALSKGFELYLAALVEPEAVEAIKLTLLTTVIAVPLNLVFGVAAAWAIAKFEFPGKQ
                     VLITLIDLPFSVSPVVAGLIYVLLFGAQGWLGPWLAAHDVKIIFAVPGIVLATVFVTF
                     PFVARELIPLMQAQGTDEEQAARVLGASGWQTFWRVTLPNVKWGLLYGVILCTARAMG
                     EFGAVSVVSGHIRGETNTIPLHVEILYNEYNFVGAFACASLLALTALLTLGLKSLVEW
                     RASRPAAGRGHTELLARAAAEAKSSAQGKDKGAPGHVLAETY"
     gene            complement(2341..3177)
                     /gene="cysT"
                     /locus_tag="PKN55_12450"
     CDS             complement(2341..3177)
                     /gene="cysT"
                     /locus_tag="PKN55_12450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012698600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysT"
                     /protein_id="HNX72100.1"
                     /translation="MSLRFKQHSVLPGFGLGLGYSLFWLCLIVLLPLAALVLKSALLG
                     LDTFWATVTTERVLASLRLSFGASLLAAAINAVFGLLVAWVLVRYPFPGKRLIDALVD
                     LPFALPTAVAGIALTTLYAPNGWFGQLLTPLGLKVAFTPLGVVVALTFIGLPFVVRTV
                     QPVLQELETELEEAAACLGASRWQTLRRVIFPAVLPALLTGFTLAFARATGEFGSVIF
                     IAGNIPLVSEITPLLIISKLEQYDYAGASAIAVLMLVISFVLLLAINLIQWWASRRPG
                     RR"
     gene            complement(3410..>3649)
                     /locus_tag="PKN55_12455"
     CDS             complement(3410..>3649)
                     /locus_tag="PKN55_12455"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNX72101.1"
                     /translation="IVKFDRSLIAGASEARRYLLGRLVSAVHALGATVVAEGVETEDE
                     AQIARDAGVDMLQGYLIGRPHGELSEGRLPLAANA"
BASE COUNT          708 a         1205 c         1165 g          571 t
ORIGIN      
        1 ctcagccaag gagaaaccat catgtccgcc tttgtccaca gcgccaccgt ccacgcccat
       61 gtgcatcctg cgcgtttcag catcggcagc ctgattgtgc tgggcggggc gctggcggtg
      121 ctgtcgcgcc tgtttgggat ggcctgaatg cccgctgcca tagccagccg gccatccccc
      181 gaaggcggat ggccggcagc atgttgatgc aagcgtggtg cgccacgcca agtgctcaga
      241 tattggcgcc ttcgtccaga aacaccgcca gcttgcgcgg gcgcacatac accgtgtcgc
      301 ccagcgccag gcgcagttgc tggtagtcgt cgcgggtcag cgcggcgtcg atcaactcgc
      361 cgctgccctg gcggcgcagc tccacccgca caatcgggcc cacgctgtgg atatgctcga
      421 tagtgccggt ggccagcgcc tggccagctt cgcggctgat ttccagatca tgcgggcgga
      481 tatacacact ggcctcgccc ttgccggccg gggcctggcc atccagcggg tgggcgtagt
      541 cgccgatttc cacatggttg gcgcccaccc ggccgtgaaa caggttcacg tcgcccaaga
      601 actgggtgac aaacgggctg gccgggtgtt cgtaaatctc ttctggccgg cccacttgct
      661 cgatcacgcc gcgattcatc accaccacgc ggtcagagac ttccagcgct tcttcctggt
      721 cgtgggtgac aaacacgctg gtgaggtgga tgtcgtcgtg cagctggcgc agccagcggc
      781 gcaagtcctt gcgcaccttg gcgtccagcg cgccaaacgg ctcgtccagc agcagcacgc
      841 gcggctccac cgccagcgag cgcgccaggg caatgcgctg gcgctggccg ccggagagct
      901 gatccgggta ggcgtcggcc agccagtcca gctgcaccag cttgagcagg cgcatcaccc
      961 gctcgcggat gtcggccttg gccggccggc tggcgcgcgg gcgcacgctc aggccaaagg
     1021 ccacgttgtc gaacacgctc atgtggcgaa acagcgcgta atgctgaaac acaaaaccca
     1081 cctggcgctc ggacaccggc cgctgggtgg cgtcctcgcc gtggaacagc acctggccgg
     1141 cgtcggcggt ttccaggccg gcgatgatgc gcagcaaagt ggttttgccg cagccggacg
     1201 gccccagcag ggccagcagc tcgccggatt ccacggtcag gctcaggtcc ttgagcacgt
     1261 cgaattcgcc gaaggttttg cgcagattgc ggatttcaat actcatgatg gttttcctgt
     1321 cgggttttgt tcgcagtgct cagtaggttt ccgccagcac atggccgggc gcgcctttat
     1381 ctttgccttg ggccgaagac ttggcctcgg cggcggcgcg cgccagcagt tcggtgtggc
     1441 cgcgcccggc ggccgggcgg ctggcgcgcc attccaccag cgacttcagc cccagcgtca
     1501 gcaaggcggt cagcgccagc agcgaggcgc aggcaaaggc gccgacaaag ttgtattcgt
     1561 tgtacaaaat ctccacatgc agcgggatgg tgttggtttc gccgcgaata tggccagaca
     1621 ccacgctcac cgcgccaaac tcacccatgg cgcgcgccgt gcacagaatc accccgtaca
     1681 gcaggcccca tttgacattg ggcagcgtca cccgccagaa ggtctgccag cccgaggcgc
     1741 ccagcacccg cgcggcttgt tcttcatcgg tgccttgcgc ctgcatcagc ggaatcagct
     1801 cgcgggcgac aaacgggaaa gtcacaaaca cggtggccag cacaatgccc ggcacggcaa
     1861 agatgatttt cacgtcatgc gccgccagcc acggccccag ccagccctgc gcgccaaaca
     1921 acagcacgta aatcaggccg gccaccactg gcgacaccga aaacggcagg tcaatcagcg
     1981 tgatcaacac ctgtttgccc ggaaactcaa acttggcgat ggcccaggcg gcggccacgc
     2041 caaacaccag attcagcggc acggcaatca ccgtggtcag caaggtcagc ttgatggcct
     2101 ccaccgcctc gggctcgacc agcgccgcca gatacagctc gaatcccttg gacaaggcct
     2161 cgtaaaacac gctgaccagc ggggtgagca gaaacaccgc cagatacccc agcgccagcg
     2221 cggtgagcgt cagtcgcacc caggccggct cggtggtgct gcggctgcgg cggtcggtcg
     2281 cagaagaaag attcatgatt cgtcctcaaa gtgggaaagc ggcggccaga tgggcggcgg
     2341 tcaacggcgg cccggacggc ggctggccca ccactgaatc aggttgatcg ccagcagcag
     2401 cacaaaagaa atcaccagca tcagcacggc aatggcgctg gccccggcgt agtcgtattg
     2461 ctccagcttg ctgatgatca gcagcggggt gatttccgac accagcggga tattgccggc
     2521 gatgaaaatc accgaaccaa actcgccagt ggcgcgggca aaggccaggg tgaagccggt
     2581 cagcagcgcc ggcagcaccg ccggaaaaat cacccggcgc agcgtttgcc agcggctggc
     2641 gcccaggcag gcggcggctt cttccagttc ggtttccagc tcttgcagca ccggctgcac
     2701 cgtgcgcacc acaaacggca ggccgataaa ggtcagcgcc accaccacgc ccagcggggt
     2761 gaacgccact ttcaggccca gcggcgtcaa cagctggcca aaccagccat tgggcgcata
     2821 cagcgtggtc agcgcaatgc cggccacggc ggtgggcagg gcaaacggca aatccaccag
     2881 cgcatcaatc agccgcttgc cgggaaacgg atagcgcacc agcacccagg ccaccagcag
     2941 gccaaacacg gcattgatgg cggcggccag cagcgaagcg ccaaacgaca gccgcaagga
     3001 agccagcacc cgctcggtgg tgacggtggc ccagaatgtg tccaggccga gcagggcgct
     3061 tttgagcacc agcgccgcca gtggcaacag gacaatcagg cacagccaga acaggctgta
     3121 gcccagcccc aggccaaagc ccggcagcac actgtgttgt ttgaatcgga gcgacatggg
     3181 gcacccggta gaagaaatcg atggggacga ttacaccggt tgccgctagg aacggtcaaa
     3241 gcttaggaat ttgtgtttta gattaaattg atctaaagag gctggccaca tcttgtgcag
     3301 ggcatcggca ggcaggcgga cacaggctgg aggtggcgtg ctcaggtcgg gggtgagcgg
     3361 ctgagcgcgg cgcagactgc gccgcatccc gctagcaccg ggcttggtgt caggcgttgg
     3421 ccgccagcgg cagtcgccct tcgctcagct caccgtgcgg gcggccgatc agatagcctt
     3481 gcagcatgtc cacgccggca tcgcgggcga tctgcgcttc gtcttcggtt tctacgcctt
     3541 cggccaccac ggtggcgccc agcgcgtgca cggcgctgac cagccggcca agcagatagc
     3601 ggcgcgcctc gctggcgccg gcaatcaggc tgcggtcgaa tttgacgat
//
LOCUS       DAOPYX010000301         3588 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_34755_length_3588_cov_14.318992, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000301 DAOPYX010000000
VERSION     DAOPYX010000301.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 3588)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3588)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3588
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34755_length_3588_cov_14.318992"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..535)
                     /locus_tag="PKN55_12460"
     CDS             complement(<1..535)
                     /locus_tag="PKN55_12460"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011134140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HNX72102.1"
                     /translation="MATPTEMTTFLAEVERRAFKQALYATHDEDAALDIVQDAMLKLA
                     DRYAHASAEELPLLFQRILQNTIRDHYRRSQVRNRWVSLFSLLRPSHGAPADGDAGLI
                     DFDPDELLSGDADEHPDSPEDWLARKQLAQQLDAALENLPLRQRQAFLLRYWEGMDVA
                     DTAAAMGCSQGSVKTHCS"
     gene            737..2497
                     /gene="ilvB"
                     /locus_tag="PKN55_12465"
     CDS             737..2497
                     /gene="ilvB"
                     /locus_tag="PKN55_12465"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014088645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HNX72103.1"
                     /translation="MQISGAEIVVRCLQEEGVEFVFGYPGGAVLEIYDAIFQQDSFRH
                     VLVRHEQAAVHAADGYSRSSNKVGVALVTSGPGATNAVTGIATAYSDSIPMVVISGQV
                     GTPAIGLDAFQEVDMVGITRPCVKHNFLVKDVNDLAATMKKAFYIARTGRPGPVVVDI
                     PKDVTIAKTDFHYPDSVNIRSYVPINKGHPGQIKKAVNLLGEAKRPFVYVGGGAVLAN
                     ASEEVRELVRLINAPCTNTLMGLGAYPGTDPQFVGMLGMHGTYEANMAMQYCDVLVAI
                     GARFDDRVISVPSHFLAQPKKIIHIDIDPSSIAKRVKVDIPIVGNVKDVLIEMIAQLK
                     AGDVRPDAEALGNWWTQVEAWRAPQSLWFDQPEDIIKPQFVVQKLWEVTGGDAIITSD
                     VGQHQMFAAQYYKFDRPKQWINSGGLGTMGFGLPAAMGAQLANPDATVACITGEASIQ
                     MNIQELSTCKQYHVPVKIVNLNNRYLGMVRQWQEFFYGNRYSESYMDALPDFVKLAEA
                     YGHVGMRIERPADVEPALREAMKLKERLVFLDFLTDQKANVFPMVGNGKGLNQMDLPP
                     HMRDLQLGVPPYDRDYGNLS"
     gene            2513..3004
                     /gene="ilvN"
                     /locus_tag="PKN55_12470"
     CDS             2513..3004
                     /gene="ilvN"
                     /locus_tag="PKN55_12470"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HNX72104.1"
                     /translation="MRHILSILIENEAGALSRVVGLFSARAYNIDSLTVSTTEDPTLS
                     RMTIVTHGSDEVIEQITKQLNKLIEVVKVIDLNESEHVEREMMLIKVRAAGKDREEMK
                     RMTDIFRGRIIDVTEKTYTVELTGTSDKLNAFIDALDRTVILETVRTGASGIGRGERI
                     LKV"
     gene            3086..>3588
                     /gene="ilvC"
                     /locus_tag="PKN55_12475"
     CDS             3086..>3588
                     /gene="ilvC"
                     /locus_tag="PKN55_12475"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011134143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004455 - ketol-acid reductoisomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HNX72105.1"
                     /translation="MQVFYDKDADLSIIKGKKVAIIGYGSQGHAHAQNLKDSGVDVVV
                     GLRKDGASWKKAENAGHKVKEVADAVKKADVVMILLPDESQPDVYRNDIAPNLKKGAA
                     LAFAHGFNIHYNQIVPPAEVDVIMVAPKGPGHTVRSEYVKGGGVPTLIAVYQDNSGKA
                     RDIALSYA"
BASE COUNT          743 a         1096 c         1102 g          647 t
ORIGIN      
        1 gggagcagtg ggttttcacg ctgccttgcg agcagcccat ggcggcggcg gtgtcagcca
       61 catccattcc ttcccaataa cgcagcaaga aggcttggcg ttgacgcaaa ggcaaatttt
      121 ccagcgcggc gtccagttgt tgcgccagtt gcttgcgcgc cagccagtct tctggcgagt
      181 cggggtgttc gtcagcatcg ccggaaagca gttcatcagg gtcaaagtca atcaggccgg
      241 cgtcgccgtc ggcgggcgcg ccatggctgg ggcgcaacag ggaaaacagg ctcacccagc
      301 ggttgcgcac ctggctgcgc cggtaatggt cacgaatggt gttttgcagg atgcgctgaa
      361 acagcagcgg cagttcttca gcggaggcgt gggcgtagcg gtcggccagc ttgagcatgg
      421 cgtcttgcac gatgtccagc gcggcgtctt cgtcgtgggt ggcgtacagg gcctgcttga
      481 acgcgcggcg ctccacttcc gccaggaagg tggtcatctc ggttggggtg gccatgaaat
      541 cgcgctgggc tctgccggtt tgctacgagc ggccatggta tcaaaagcca cgccgggccg
      601 gtagccgggg cgggcgggcc gaatgggtcg gatgtcgcca tgttgcagcc caacaaatgg
      661 cttgaccatg ctttgccgga taagataagt tcattttttt acaagggcat gaactagcag
      721 gacttcaaga ggcattatgc agatctcggg cgcggaaatc gtcgtgcgct gcttgcagga
      781 ggaaggcgtc gaatttgttt tcggctaccc cggcggcgcg gtgttggaaa tctatgacgc
      841 tatctttcag caagatagct tcagacatgt gttggtgcgt cacgaacagg cggccgtaca
      901 cgcggctgac gggtattcgc gctccagcaa caaggtgggc gtggcgctgg tgacttccgg
      961 tccgggcgcc accaatgcgg tcaccggcat tgccaccgcc tactcggatt cgattccgat
     1021 ggtggtgatc tccggtcagg tgggcacgcc ggccattggc ctggacgcct tccaggaagt
     1081 ggacatggtg ggcatcaccc gcccgtgcgt gaagcacaac ttcctggtca aggacgtcaa
     1141 cgacctggct gccaccatga aaaaggcgtt ttacatcgcc cgcactggcc ggcctggccc
     1201 ggtggtggtg gacatcccca aggacgtcac catcgccaag accgacttcc actacccgga
     1261 ttcggtcaat atccgctcgt atgtgccgat caacaagggc catccgggtc agatcaaaaa
     1321 agccgtcaac ctgctgggcg aggccaagcg cccgtttgtg tatgtcggcg gcggcgccgt
     1381 gctggccaac gccagcgaag aagtgcgcga gctggtgcgc ctgatcaacg ccccgtgcac
     1441 caacacgctg atgggtctgg gcgcctatcc gggcaccgac ccgcagtttg tcggcatgct
     1501 gggcatgcac ggcacttacg aagccaatat ggccatgcag tactgtgatg tgctggtggc
     1561 catcggcgcg cgctttgacg accgcgtgat cagcgtgcca tcgcactttc tggcgcagcc
     1621 gaaaaaaatc attcacatcg acatcgaccc gtcgtcgatc gccaagcgcg tgaaggtgga
     1681 catccccatc gtcggcaacg tcaaggacgt gctgatcgag atgatcgccc agctcaaggc
     1741 tggcgatgtg cgcccggacg ccgaggcgct gggcaactgg tggacgcagg tggaagcctg
     1801 gcgcgcgccg cagtcgctgt ggtttgacca gccggaagac atcatcaagc cgcaatttgt
     1861 ggtgcaaaag ctgtgggaag tgaccggtgg cgacgccatc atcacctccg acgtcggcca
     1921 gcaccagatg tttgccgccc agtattacaa gttcgaccgc cccaagcagt ggatcaactc
     1981 cggcggtctg ggcaccatgg gctttggcct gccggccgcc atgggcgcgc aactggccaa
     2041 cccggacgcc accgtggcct gcatcaccgg cgaagcgtcg atccagatga atattcagga
     2101 actctccacc tgcaagcagt accatgtgcc ggtgaagatc gtgaacctga acaaccgtta
     2161 tctgggcatg gtgcgccagt ggcaggaatt cttctacggc aatcgttatt ccgaatccta
     2221 catggacgcg ctgcctgact tcgtcaagct ggccgaggcc tatggccatg tcggcatgcg
     2281 cattgagcgc ccggccgacg tcgagccggc gctgcgcgaa gccatgaagc tgaaagaacg
     2341 cctggtgttc ctcgacttcc tcaccgacca gaaggccaac gtcttcccga tggtgggcaa
     2401 tggcaagggc ctgaaccaga tggatctgcc cccgcacatg cgcgatctgc agctgggcgt
     2461 tccgccctac gaccgcgact acggcaacct gagctaagaa gaacacggca ccatgcgaca
     2521 cattctttcc atcctgattg aaaacgaagc cggggcgctg tcccgcgtgg tcgggctgtt
     2581 ttccgcgcgc gcctacaaca ttgactcgct gaccgtctcc accaccgaag acccgaccct
     2641 gtcgcggatg accatcgtca cccacggctc ggacgaggtg atcgagcaga tcaccaagca
     2701 gctgaacaag ctgatcgaag tggtgaaggt gatcgacctg aacgaatccg agcacgtcga
     2761 gcgcgaaatg atgctgatca aggtccgcgc cgccggcaag gaccgcgaag aaatgaagcg
     2821 gatgacggat attttccgtg gccgcattat cgacgtgacc gaaaagacct acactgtcga
     2881 gctgaccggc acgagcgaca agctcaatgc cttcatcgac gccctggacc gcacagtgat
     2941 cctggaaacg gtccgcaccg gcgcatccgg cattggtcgc ggcgaacgca tcctcaaggt
     3001 gtgacgccgc ttaacaatcg caccccgccg ggcaatcttg cccggcattg aactttattt
     3061 acagaaacag caggggacac ccaacatgca agtgttttac gacaaggacg ccgatctctc
     3121 catcatcaag ggcaagaagg tcgccatcat cggttacggc tcgcaaggcc acgcccacgc
     3181 ccagaacctg aaggattccg gcgtggacgt cgtggtgggc ctgcgcaagg acggcgcctc
     3241 ctggaaaaaa gccgagaacg ctggccacaa ggtcaaagaa gtcgccgacg ccgtgaaaaa
     3301 ggccgacgtg gtgatgattc tgctgccgga cgaatcccag ccggacgtct accgcaacga
     3361 catcgccccg aacctgaaaa agggcgccgc cctggcgttt gcccacggct tcaacatcca
     3421 ttacaaccag atcgtgccgc cggctgaagt cgacgtgatc atggtggccc cgaaaggccc
     3481 gggccacacc gtgcgctccg aatacgtcaa gggcggcggc gtgccgaccc tgatcgctgt
     3541 ctaccaggac aactccggca aggcccgcga catcgccctg tcctacgc
//
LOCUS       DAOPYX010000302         3587 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_34766_length_3587_cov_12.816251, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000302 DAOPYX010000000
VERSION     DAOPYX010000302.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 3587)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3587)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3587
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34766_length_3587_cov_12.816251"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..212
                     /locus_tag="PKN55_12480"
     CDS             <1..212
                     /locus_tag="PKN55_12480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX72106.1"
                     /translation="HVMRAMGIPYTAAHGTIRFSLSRYNTEAEVDRVIAAVPDIIAQL
                     RKLSPYWGEKGPSANPEASFAPTYA"
     gene            322..861
                     /locus_tag="PKN55_12485"
     CDS             322..861
                     /locus_tag="PKN55_12485"
                     /inference="COORDINATES: protein motif:HMM:NF006739.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HNX72107.1"
                     /translation="MSQIGVFFGTTSGSTRKVAKMIKKRFDDAVMAAPLNINRTDAEV
                     FASYPLLLLGTSTVGEGQLPGASADSEASWEEAMAKLAGVDLSGKTVALFGLGDQSRY
                     PERFASGLRDLYEFVISKGAAVVGQWPTEGYEFEQSRAVVDGQFVGLVLDLSNQSQLT
                     EARLDAWLKQIAPVFGLPG"
     gene            complement(907..2610)
                     /locus_tag="PKN55_12490"
     CDS             complement(907..2610)
                     /locus_tag="PKN55_12490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012607077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNX72108.1"
                     /translation="MKVSIERGHFEALLAEFPRLQPWAAQLRFGNRVEMAFADLHDAE
                     LAQLVARYQAGGVTLRAQAAQLLTLQQALAAGGARFGEDELDALAPALAHYLIDGALR
                     GWLFSAQITGKPLAYVVSRLDYTPPSEDETGKVFIELKANSRARLATVTVRLSAADIA
                     GRTLAEALAHKGFVKETPALLASYDEAAERYFDWRARYGAQFSARGVGWFADNPTATH
                     RDTDYARKDVVILSSTGQPARLVNDEGILGERPLALDATGDILSKFLRRLTRSNLLSS
                     RQEADLETLQGQMPRGAFTELPVHFYILTFHLDLHHYVWVHVDDMAPYVYQPALKTKL
                     VLPPQQTDLIDILTAEMDVLMDDIVAGKSGGTTVLCAGPAGVGKTLTAEVYAEIIQRP
                     LYRVHSGQLGLNVGAMETALKEVLTRAQRWGAVMLIDEADVYIKRRDDNITMNAVVGV
                     FLRVLEYFNGLLFLTTNRVDDIDEAIVSRCIALIRFHPPQHDDRRRIWAVMSEQFGLA
                     LDPALMDELASRFPAATGRDIKGLAKLVAKYCHHKAVAPTLEVFIRCSAFRGMDVVGA
                     G"
     gene            2901..3329
                     /locus_tag="PKN55_12495"
     CDS             2901..3329
                     /locus_tag="PKN55_12495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018150282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNX72109.1"
                     /translation="MLTVRQLLKEKPAGFFSVPLDATVLQALQVMAEHNVGAVLVMEG
                     ESLHGIFSERDYARRVVLLGKTSATTLVGEIMTNKVCYIGPEHTVDQCMALMTDKNIR
                     HLPVLEHGAVLGMVSMRDVVRATLDAQAQTIEQLVSYIQQ"
BASE COUNT          646 a         1133 c         1126 g          682 t
ORIGIN      
        1 cgcatgtgat gcgcgccatg ggcatcccct acaccgccgc ccacggcacc atccgcttct
       61 cgctgtcgcg ctacaacacc gaagccgaag tcgatcgggt gattgccgcc gtgccggaca
      121 tcatcgccca actgcgcaag ctgtcgccgt actggggcga aaaaggcccg tcggccaacc
      181 cggaagccag ttttgcaccg acttatgcct gatgttttct tccctccggc aatgctgcgt
      241 attgcctgaa ttgagatccc cggccccacc ggggattttt ttcatcaccc caccatgact
      301 gactataagg agagtctcat catgtcccag attggcgtat tttttggcac cacctccggc
      361 tccacccgca aagtggcgaa aatgatcaaa aaacgctttg acgacgccgt gatggccgcg
      421 ccgttgaaca tcaaccgcac cgacgccgag gtattcgcca gctaccccct gctgctgctg
      481 ggcacctcca ccgtgggcga aggccaactg cccggcgcca gcgccgacag cgaagccagc
      541 tgggaagaag ccatggccaa actggccggc gtggacctca gcggcaaaac cgtggcgctg
      601 ttcggcctgg gcgaccaaag ccgctacccc gagcgctttg ccagcggcct gcgcgacctg
      661 tatgagtttg tgatcagcaa aggcgccgcc gtggtcggcc aatggccaac cgaaggctat
      721 gaattcgagc aatcgcgcgc cgtggtggac ggccagtttg tcggcctggt gctggatttg
      781 tccaaccaat cgcaactcac cgaagcccgg ctggacgcct ggctgaagca gattgccccg
      841 gtgtttggtt tgccggggta aggcgttgcg ctgcccgtgc aggaaggaca tcctgcacgg
      901 gtactctcac cccgccccca ccacatccat cccccgaaac gccgagcagc ggataaacac
      961 ctccagcgtc ggcgccaccg ccttgtggtg gcagtatttg gccaccagtt tggccaggcc
     1021 tttgatgtcg cggccggtgg cggcgggaaa gcggctggcc agttcgtcca tcagcgcggg
     1081 gtcaagcgcc aggccaaatt gctcgctcat caccgcccag atgcgccggc ggtcgtcgtg
     1141 ttggggtggg tgaaagcgga tcagggcgat gcagcgcgag acaatcgcct cgtcgatgtc
     1201 gtccacccgg ttggtggtga gaaacagcag gccgttgaaa tactccagca cgcgcaggaa
     1261 cacgccgacc acggcgttca tggtgatatt gtcgtcgcgg cgtttgatat acacatcggc
     1321 ctcgtcgatc agcatcaccg cgccccagcg ctgggcgcgg gtgagcactt ctttcagcgc
     1381 ggtttccatg gcgccgacgt tcagccccag ttggccagag tgcacgcggt agagcgggcg
     1441 ctggatgatt tcggcgtaca cctcggcggt cagggttttg cccaccccgg ccgggccggc
     1501 gcacagcacg gtggtgccgc cggatttgcc ggcgacgatg tcgtccatca gcacgtccat
     1561 ttcggcggtg aggatgtcga tcaggtcggt ctgctgcggc ggcagcacca gcttggtttt
     1621 cagcgccggt tgatagacat acggcgccat gtcgtccaca tgcacccata catagtggtg
     1681 cagatccagg tgaaaggtga ggatgtaaaa atgcaccggc agctcggtga acgcgcccct
     1741 gggcatctgg ccttgcaggg tttccaggtc ggcttcctgg cggctgctca acagattgct
     1801 gcgggtgagc cggcgcagaa atttgctgag tatgtcgccc gtggcgtcca gcgccagcgg
     1861 gcgttcgccc agaatgcctt cgtcgttgac cagccgcgcc ggctggccgg tggacgacag
     1921 aatcaccaca tccttgcgcg cgtagtcggt gtcgcggtgg gtggcggtgg ggttgtcggc
     1981 aaaccagccc accccacgcg cagaaaactg cgcgccgtag cgcgcgcgcc agtcaaaata
     2041 gcgctcggcg gcttcgtcgt agctggccaa cagcgccggg gtttctttga caaaaccctt
     2101 gtgcgccagc gcctcggcca gggtgcgccc ggcaatgtcg gcggccgaca ggcgcacggt
     2161 gacggtggcc agccgcgcgc gtgaattggc cttgagctcg ataaacactt tgccggtttc
     2221 gtcttccgat ggcggggtgt aatccagccg gctcaccaca taggccagcg gcttgccggt
     2281 gatttgcgcc gaaaacagcc agccgcgcag cgcgccgtca atcagataat gcgccagcgc
     2341 cggggccagc gcgtccagct cgtcttcgcc aaagcgcgcg ccgcccgccg ccagcgcctg
     2401 ttgcagggtg agcagctgcg ccgcctgcgc gcgcagggtg acgccgccgg cctgatagcg
     2461 ggccaccagc tgcgccagct cggcgtcgtg caaatcggca aacgccatct ccacccgatt
     2521 gccaaagcgc agctgcgccg cccagggttg caatcgggga aactcggcca acaaggcttc
     2581 aaagtgcccg cgctcgatgc tgactttcat ggcgcttacc tgtgtgcaaa agaaagtgtg
     2641 cgtgcagcga gttttgtgcc agacaaatat atttttaaaa acaatggatt gaattttttt
     2701 gtggcgtggc ttgtcaagca tcgtacagcc aggctgtacc ccggtggacg tgcctacagc
     2761 atgccgcaac ttgccctgca ttccagccgg atagcggcta taactcaaaa gcacgcctgt
     2821 cgccggcctg gcgacctgct gagccctggc gccgtccttt gcgccccttg cctatcgtat
     2881 gacgcaacca aggagagacc atgctgaccg ttcgccaatt gttgaaagaa aaacccgccg
     2941 gtttttttag cgtgccgctg gacgccaccg tgctgcaagc cctgcaagtg atggccgagc
     3001 acaatgtggg cgctgtgctg gtgatggagg gggaaagcct gcacgggatt ttttccgagc
     3061 gtgattacgc ccgccgcgtg gtgttgttgg gcaagacttc ggccaccacg ctggtgggcg
     3121 aaatcatgac caacaaggtg tgttatatcg gccccgagca tacggtggat cagtgcatgg
     3181 cgctgatgac tgacaaaaac atccgccact tgccggtgct ggagcatggc gctgtgctgg
     3241 gcatggtgtc gatgcgcgat gtggtgcgcg ccacgctgga tgcccaggcg caaaccattg
     3301 agcaactggt gagttacatc cagcagtaag agccactcac catcccctgg cgttgtgggg
     3361 tgactttgcc gtacgacttg tactgtctgc ggtcacacgc ctggccaggg ccgctgcgct
     3421 ggctttggtg ggcgcgctaa gtgttgctta ccccgtgcgc tttggcccga atggcggccg
     3481 gcgctgggga gcgcgattgc cgtcctggcc agtctgctat ttgcccgcct gtcgcagccc
     3541 ggccgccagc cagcgctgaa tgctggcggc gtccatcgcg ccggcct
//
LOCUS       DAOPYX010000303         3581 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_34840_length_3581_cov_12.449234, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000303 DAOPYX010000000
VERSION     DAOPYX010000303.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 3581)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3581)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3581
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34840_length_3581_cov_12.449234"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..303)
                     /locus_tag="PKN55_12500"
     CDS             complement(<1..303)
                     /locus_tag="PKN55_12500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018746616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HNX72110.1"
                     /translation="MSLAKRIIPCLDVTAGRVVKGVNFVGLRDAGDPVEIARRYDEQG
                     ADELTFLDITASSDDRDIILHIIEAVAEQVFIPLTVGGGVRKVEDVRRLLNAGADKV"
     gene            complement(340..1080)
                     /gene="hisA"
                     /locus_tag="PKN55_12505"
     CDS             complement(340..1080)
                     /gene="hisA"
                     /locus_tag="PKN55_12505"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009115982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HNX72111.1"
                     /translation="MLLIPAIDLKDGQCVRLKQGVMDDATVFSDDPVKVAAHWRDQGA
                     RRLHLVDLNGAFAGKPKNLAVIRDILAEVGDDMPVQLGGGIRDLDTIEKYLDMGLQYV
                     IIGTAAVKTPGFLHDACDAFPNQVIVGLDAKDGKVATDGWAKITHHDVIDLARRFQDY
                     GVVSVIYTDIGRDGMMSGVNIDATVKLAQALTIPVIASGGLTNLDDVRALCGVEHEGI
                     EGAITGRAIYEGSINFREAQDLADALGQ"
     gene            complement(1111..1767)
                     /gene="hisH"
                     /locus_tag="PKN55_12510"
     CDS             complement(1111..1767)
                     /gene="hisH"
                     /locus_tag="PKN55_12510"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012695678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HNX72112.1"
                     /translation="MSAAKPTIAVVDYGMGNLRSVTKALEHVANGRAHITLTAQPEVV
                     RAADKVVFPGQGAMKDCMAGLLESGLKDAVLEAAANKPFFGVCVGAQLLLDHSEEGDT
                     PALGLYAGQVKRFPDGLSDAAGARLKVPHMGWNEVWQTARHPLFAGVADGSRFYFVHS
                     YYMAPADAALSLGYAEYPFAFSAVIGRDNVFATQFHPEKSAAAGLKLLENFIAWDGQR
                     "
     gene            complement(1764..2357)
                     /gene="hisB"
                     /locus_tag="PKN55_12515"
     CDS             complement(1764..2357)
                     /gene="hisB"
                     /locus_tag="PKN55_12515"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HNX72113.1"
                     /translation="MRQADITRNTLETQITVSINLDGDGHGTFATGVPFLEHMLDQIC
                     RHGLIDLNIQAVGDLHIDAHHTVEDIGITLGQAFAKAIGDKKGIRRYGHAYVPLDEAL
                     SRVVIDLSGRPGLVYNIDFTRAAIGQFDVDLFSEFFHGFVNHAMVTLHIDNLRGVNAH
                     HQAETVFKAFGRALRMAVEPDPRMAGVTPSTKGTLTA"
     gene            2512..3573
                     /gene="hisC"
                     /locus_tag="PKN55_12520"
     CDS             2512..3573
                     /gene="hisC"
                     /locus_tag="PKN55_12520"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018747095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HNX72114.1"
                     /translation="MSQYWSAVVHGLTPYVPGEQPKLPDLVKLNTNENPYGPSPKALQ
                     AIAAETSDHLRLYPDPNGDSLKLAVAAHYGVTTQQVFVGNGSDEVLAHVFLALLKHDE
                     PILFPDITYSFYPVYCGLYGVEYREVPLDADFAINPDDYHGPNGGVIFPNPNAPTGRL
                     LPLDAVAAIARHNPDRVVVVDEAYIDFGGDSAIRLIDQHPNLLVTQTLSKSRSLAGLR
                     VGFAVGHPDLIDALERVKNSFNSYPLDRLALAGATAAMNDNAHFEHTRQAVIATRERL
                     NAGLAQLGFAVLPSAANFVFARHPQHDAAQLAAALRERAIIVRHFKHPRIDQFLRITV
                     GTEQQTDVLLAALAEILAV"
BASE COUNT          661 a         1190 c         1083 g          647 t
ORIGIN      
        1 caccttgtcg gcgccggcat tgagcagccg gcgcacgtct tccaccttgc gcacgccgcc
       61 gcccaccgtc agcgggatga acacctgttc ggccaccgct tcgatgatgt gcaggatgat
      121 gtcgcggtcg tcgctggagg cggtgatgtc cagaaaagtc agctcgtcag cgccttgctc
      181 gtcgtagcgc cgggcgattt ccaccgggtc gccggcgtcg cgcaggccga caaaattgac
      241 gcccttgacc acgcgaccag cggtcacgtc cagacagggg atgatgcgtt tggctaaaga
      301 catggcgaat gcattctgtg ttgtgcgggc agtccgggct tattggccca gcgcgtcggc
      361 caggtcctgc gcctcgcgga agttgatgct gccctcgtaa atggcgcggc cggtgatggc
      421 gccctcgatg ccttcgtgct caacgccgca cagcgcgcgc acatcgtcga ggttggtcag
      481 gccgcccgag gcgatcaccg gaatggtcag cgcctgcgcc agcttgacgg tggcgtcgat
      541 gttcacgccg ctcatcatgc cgtcgcggcc gatgtcggtg taaatcaccg acaccacgcc
      601 gtaatcctga aaacggcggg ccaggtcgat cacgtcgtgg tgggtgatct tggcccagcc
      661 gtcggtggcc actttgccat ctttggcgtc caggccaaca atcacctgat tggggaaggc
      721 gtcgcaggcg tcgtgcagaa agccgggggt tttcaccgcc gccgtgccga tgatgacgta
      781 ttgcaggccc atatcgaggt atttctcgat ggtgtccagg tcgcggatgc cgccacccag
      841 ctgcaccggc atgtcatcgc cgacttcggc gaggatgtcg cggatcaccg cgaggttttt
      901 cggcttgccg gcaaaggcgc cgttcagatc aaccagatgc aggcgacgcg cgccctggtc
      961 gcgccagtgg gcggcaacct tgaccgggtc atcagaaaac acggtcgcat cgtccataac
     1021 gccttgtttc aggcgcacac actggccgtc tttcagatca atcgcgggaa tcagcagcat
     1081 gatgtatctc gggcttttgg gtcttcgggg ttatcgctgg ccatcccagg caatgaagtt
     1141 ttccagcaac ttcaagccgg cggccgcgct tttttcgggg tgaaactgcg tggcgaacac
     1201 attatcacga ccaatgacgg cggaaaatgc gaaaggatac tcagcatagc ccaaggacag
     1261 cgcggcatcg gccggcgcca tgtagtagct atgcacaaaa taaaagcggc tgccgtcggc
     1321 cacgccggca aacagcgggt ggcgggcggt ctgccagact tcgttccagc ccatatgcgg
     1381 caccttcagg cgcgcgccag cggcgtcgct cagcccatcc ggaaagcgct tgacctggcc
     1441 ggcgtacagc cccagcgccg gcgtgtcgcc ttcttcgctg tggtccagca gcagttgcgc
     1501 gcccacgcac acgccaaaaa acggcttgtt ggcggcggct tccagcacgg cgtctttcag
     1561 gccggattcc agcaggccgg ccatgcaatc cttcatcgcg ccctgaccgg gaaacaccac
     1621 cttgtcggcg gcgcgcacca cctcgggttg cgcggtcagc gtgatgtggg cgcggccgtt
     1681 ggccacatgc tccagcgcct tggtcaccga gcgcagattg cccatgccgt agtccaccac
     1741 ggcgattgtc ggtttggcgg cgctcatgcg gtgagggttc ctttggtgga cggggtgacg
     1801 ccggccatgc gcgggtctgg ctcaacggcc atgcgcagcg cgcggccaaa ggccttgaac
     1861 accgtttcgg cctgatgatg ggcgttcacg ccgcgcagat tgtcgatatg cagcgtcacc
     1921 atcgcgtgat tgacaaagcc atggaagaat tccgagaaca aatccacgtc aaactggcca
     1981 atcgccgcgc gggtgaaatc aatgttgtac accaggccag ggcggccgga gaggtcaatc
     2041 accacccgcg acagcgcctc gtccagcggc acataggcgt ggccataacg gcgaatgccc
     2101 tttttgtcgc caatcgcctt ggcgaaggcc tggcccaggg tgatgccgat gtcttccacg
     2161 gtgtggtgcg cgtcgatatg caaatcgccc accgcctgaa tattcaggtc gatcaggcca
     2221 tggcggcaaa tctggtcgag catatgctcc agaaacggca cgccggtggc gaaggtgccg
     2281 tgaccgtcgc cgtccaggtt gatcgacacg gtgatctggg tttccagggt gttgcgggta
     2341 atgtcagcct gacgcatggg atccatcact agataaacaa ggcaaaccgg cgcggcgcgc
     2401 cagtctgatc cagacctgag gccgcgccgc acagccctgc catgatacca tttcaccgga
     2461 aatcatttca cggggattgc aatactcgcc ccgccagcca ggaacccgac catgagccaa
     2521 tactggagcg ccgtcgtcca cggcctgacc ccctacgtgc ccggcgagca gcccaagctg
     2581 cccgatctgg tcaaactcaa caccaacgaa aacccctacg gcccctcgcc caaggcgctg
     2641 caagccattg ccgccgaaac cagcgaccat ctgcgcctgt accccgaccc caacggcgac
     2701 agcctgaaac tggccgtggc cgcccactat ggcgtgacca cccagcaagt gtttgtcggc
     2761 aatggctcgg acgaagtgct ggcccatgtg tttctggccc tgctcaagca cgacgagccg
     2821 attctgtttc cggacatcac ctacagcttt tatccggtgt actgcggcct gtacggcgtg
     2881 gaataccgcg aagtgccgct ggacgccgac tttgccatca atccggacga ctaccacggc
     2941 cccaatggcg gggtgatctt ccccaacccc aacgccccca ccggccgcct gctgccgctg
     3001 gacgccgtgg ccgccatcgc ccgccacaac cccgatcgcg tggtggtggt ggacgaagcc
     3061 tatatcgact ttggcggcga cagcgccatc cgcctgatcg accagcaccc caacctgctg
     3121 gtgacgcaaa ccttgtccaa atcccgctcg ctggccggcc tgcgcgtggg ctttgccgtc
     3181 ggccaccccg acttgatcga cgcgctggag cgggtgaaaa acagctttaa ctcctacccg
     3241 ctcgaccgcc tggccctggc cggcgccacc gccgcgatga acgacaacgc ccactttgag
     3301 cacacccgcc aggcagtgat cgccacccgc gaacgcctga acgccggcct ggcgcaactg
     3361 ggctttgccg tgctgccctc cgccgccaac tttgtgtttg cccgccaccc gcaacacgac
     3421 gccgcccaac tggccgccgc cctgcgcgaa cgcgccatca tcgtgcgcca cttcaaacac
     3481 ccgcgcattg accagttttt gcgcatcacg gttggcacgg aacagcagac ggatgtgttg
     3541 ttggcggcgt tggcggagat tttggcggtg taagggctgg c
//
LOCUS       DAOPYX010000304         3570 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_35003_length_3570_cov_4.120626, whole genome shotgun sequence.
ACCESSION   DAOPYX010000304 DAOPYX010000000
VERSION     DAOPYX010000304.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 3570)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3570)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3570
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35003_length_3570_cov_4.120626"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..146
                     /locus_tag="PKN55_12525"
     CDS             <1..146
                     /locus_tag="PKN55_12525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase"
                     /protein_id="HNX72115.1"
                     /translation="VHALPLPGTRYDCGSKLGYLKASVDYALKHHEVGTAFAHWLSAH
                     PAR"
     gene            852..2084
                     /locus_tag="PKN55_12530"
     CDS             852..2084
                     /locus_tag="PKN55_12530"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HNX72116.1"
                     /translation="MKPRFSYLHSAVLAVSVALPQAVMATPAGDVLLAVGPAYVQTAN
                     GEVHPARKGLQVAAGERIMTRTGGYVHVRMRDGGLVAIRPDSVLDIQVFEYPVDHPEK
                     GRVRYRLQQGVARSVTGAIGEANKEAFRMNTPVAAIGVRGTDFVTQTDTLVTRVSINS
                     GAVVVAPFANNQGCKAEGFGACLSNALVLRAGKEVGYVEVGAQDGAPRLRKEQRLPLP
                     PAHPDEPVAILQENRAARLEQGIGVQTAKLPDSVHWGRWSVAEGSASPSVMDLVQAGK
                     SIHLVNFLFGVGVERLPDTLPSQGSIGFQLAGGEAVVRQLDGAYSPASLASGQLRVDF
                     TSQTFQANAKVTESNAQHVLTAAGRVDRPGYLIADPTLSNSALHTVLNHDLNTAGSVF
                     TKTLDDGRTVLGSAIWKR"
     gene            2081..>3570
                     /locus_tag="PKN55_12535"
     CDS             2081..>3570
                     /locus_tag="PKN55_12535"
                     /inference="COORDINATES: protein motif:HMM:NF017076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE2 domain-containing protein"
                     /protein_id="HNX72117.1"
                     /translation="MTNIHWQRQRAWLFAFVLASILLLPWVDVLDEWFADWRMHITGQ
                     MTAPQASPVAVVAIDEATLDNFGPWPWPRTTLANVLEHIVRHYQPAAIGLDIVFPPNA
                     QHLEDVALAKILQTHPIVVGQLFDQTGLQRGVPAYQAVTQNDSARLPEYSGVLASSAQ
                     LGVVAAGHINGLLDHDGKMRRAYPYVCGQHGCSPSLAARVLGVVANLEHWTLSKNPKD
                     NTPWLLTPDEMPHLALALDQDYAVTLPWRATPGYATFSLADLWHAKIPTEVLTNKILI
                     IGATAHGMGDKVITPINALTPGVEAHATLLTAWLDQSLPRPSPHAKIFVFVMLICQAV
                     ILLLLRNPWHHGVATMVMSLAPVWLSLYLYQQGWIFPVASNAIYPWMLFVYTLTAHAI
                     RRYQDLILRFSAYLPEPLVKSLTRQDSIDRAEVKWTTCLYADIMGYTHTSQQLAPEKL
                     AAWCNQAIDLVVVEVSQHQGMVDNIAGDGLLAYWRGQPADQQAQLA"
BASE COUNT          815 a          842 c         1015 g          898 t
ORIGIN      
        1 cggtgcatgc cctgccgctg cctggaacgc gctatgattg cggttccaaa ctgggttatc
       61 tgaaagccag tgtggattat gcgctgaaac accacgaggt gggcacggcc tttgcccact
      121 ggctgagcgc gcatccggca cgctgaatgc aaaaccgcgc tgtacatttg cttgaaaacg
      181 ctggcaaagt gttagagtcg ctcacagaac ccagtgtaac ggccctggct agtaccagtc
      241 acgttgaaac acgagttcat gcgccacgaa gaacccctcg ccgcaccaca ccgcgtaatt
      301 cccgcgcggg cgggaatcca ggtgtgccca cagcgtgctg acggtccgca cgctcaatgc
      361 caccgcgcac gctgtggcgc ggtctgggtt cccgccttcg cgggaacgac acagggggtg
      421 aaactcatgc tggacacgct ctaagagcgg ctaacaaaac ccagcgaagc gcgcttggct
      481 aggcgcgcgg ccgcagacag tacaaggcgt acggcaaggc cgtgcaatga tgccagaagg
      541 gttttgtgag cggcgcttaa ggtggtgtgt tcatgtattg ctggagtagc atatactgtg
      601 taaattcacc atggcaaaac tgctgaagaa aacgcttttt tattaagaat caatggattg
      661 cctgaaaact cttttccact tagcggcgcg aaataaaacc cggcgcagtg atcctggcta
      721 ggcgtgcggt cgcagatagt acaagcagta cggccaggcg tcgcaacgac gccaggaggg
      781 ttttgtcagc ggctttgagt gaattgtggt attgggtagg gtaaattttg gttttggttg
      841 gagggtgtgc gatgaaaccc cgcttttctt acttgcatag tgcagttctc gctgtcagtg
      901 ttgcgctccc gcaggctgtt atggcaacgc ctgctggtga tgtgctgctc gcggttggtc
      961 ctgcttatgt gcagacggcc aacggtgagg tacatcctgc tcgcaagggc ctacaggttg
     1021 ctgcgggtga acggattatg actcggacag gcgggtatgt tcatgtgcgg atgagagatg
     1081 gtggcctggt ggctattcgc cctgattctg tgttggatat ccaggtgttc gagtacccgg
     1141 tcgatcatcc tgaaaaaggc cgagtgcgct atagattaca gcagggtgtt gctcgctcgg
     1201 taaccggagc aatcggtgag gccaataaag aagcattcag aatgaataca ccagtggcgg
     1261 cgattggtgt gcgtggtacc gattttgtta cccagacaga taccttggtg acgagggtca
     1321 gcatcaatag cggcgctgtg gtggtggctc cttttgccaa caaccaaggt tgcaaggcgg
     1381 aaggatttgg cgcgtgctta agtaatgcat tagtgttacg tgcgggcaag gaagtaggat
     1441 atgtggaggt aggcgctcaa gatggtgccc cacgattgcg taaagagcag cgtttgccgt
     1501 taccccctgc ccatcccgat gagcctgtag ccattttgca ggaaaatcgt gccgcccgcc
     1561 tggagcaggg tattggtgtt caaacagcca agctgccaga tagtgtacat tggggacgct
     1621 ggagtgtagc cgaaggcagt gcgtctccga gcgtgatgga tctggttcag gcaggaaaat
     1681 caattcactt ggtcaatttt ttatttggtg tcggcgtaga gcgtctcccg gatactttac
     1741 ctagccaagg gagtattggt tttcagctgg caggcggtga agccgtggtg cgtcaattgg
     1801 atggtgcata tagtccagca tcattggcgt ctggccagtt acgagtggat tttacttcgc
     1861 aaactttcca agcaaatgcc aaagtgacag aatccaatgc acaacatgtg ctcactgcgg
     1921 cggggcgtgt ggatcgtcca ggctatttga ttgccgatcc aacattatct aattcagcgc
     1981 tacatacagt gctcaatcat gatctgaata cagcaggtag tgtgttcacc aaaaccctgg
     2041 atgatggccg gacagtttta ggtagtgcaa tctggaaacg ttgaccaata tccattggca
     2101 aagacagcgg gcttggctgt ttgcctttgt gctggcaagt attttgttgc tgccatgggt
     2161 agatgtgctt gacgagtggt ttgctgattg gcgaatgcat attacgggac agatgacggc
     2221 acctcaggct tcacctgtcg ccgtcgtggc cattgatgaa gccaccttgg ataattttgg
     2281 tccgtggcca tggcctcgta caacgcttgc taatgtactt gagcatattg tacgtcatta
     2341 tcagcctgcc gctattggcc tggatattgt gtttccgcct aatgctcagc atctggaaga
     2401 tgtggcattg gcaaagattt tacagacaca cccaattgtg gttggccaat tgtttgacca
     2461 aacagggttg cagcgggggg tgcctgctta tcaagcggtt actcaaaatg actcagctcg
     2521 tttgcctgag tatagcggtg tattggcaag cagtgcgcag cttggtgtgg tcgcagcagg
     2581 ccatatcaat ggcttgctgg atcatgacgg aaagatgcgc cgagcttatc catatgtttg
     2641 tggacagcat ggttgtagcc caagtctggc tgcacgagtg ttaggcgtgg ttgcgaacct
     2701 tgagcattgg acgcttagca aaaatcccaa agataataca ccatggctgt taacaccaga
     2761 tgagatgcca catttggcgc tggcattaga tcaagattat gccgttacac taccttggcg
     2821 agccacacca ggctacgcaa ctttttcgtt agcagatctt tggcatgcaa aaattcctac
     2881 cgaggtgctg actaataaga tattaatcat cggcgcaacg gcccatggca tgggagacaa
     2941 ggtcattacg ccaattaatg cgttaacccc cggcgtagag gcgcatgcga cgttgttaac
     3001 tgcgtggctg gatcaatccc tacccaggcc atctccacac gcaaagattt ttgtatttgt
     3061 catgctaata tgtcaagccg tcatcttgct tttattaaga aacccatggc accatggcgt
     3121 cgcgaccatg gtcatgagct tagctccagt gtggcttagc ttatatcttt atcaacaggg
     3181 atggattttt cccgttgcat ctaatgcaat ttacccctgg atgttgtttg tctatacgct
     3241 tactgcgcat gcgatacgcc gataccaaga tttgatcctg cgctttagtg cgtacctgcc
     3301 cgagccattg gttaaatctt tgactcgaca agattcgatt gatcgtgctg aggtaaaatg
     3361 gacaacatgc ctatatgcag atattatggg ctacacgcat acgtctcagc agcttgcgcc
     3421 ggaaaagctc gccgcgtggt gtaatcaagc cattgatctg gttgtggttg aggtgagcca
     3481 acatcagggc atggtggata atattgctgg cgatggttta ctggcctact ggcgtgggca
     3541 acctgctgac caacaagcac aacttgcatt
//
LOCUS       DAOPYX010000305         3563 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_35105_length_3563_cov_6.990023, whole genome shotgun sequence.
ACCESSION   DAOPYX010000305 DAOPYX010000000
VERSION     DAOPYX010000305.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 3563)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3563)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3563
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35105_length_3563_cov_6.990023"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            205..435
                     /locus_tag="PKN55_12540"
     CDS             205..435
                     /locus_tag="PKN55_12540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX72118.1"
                     /translation="MQLTSRQISPRLRRMLRQCWQRLSERDLDQIVNRKSLLAALRRR
                     YTRPVVEIEQEIDQFFRTVFAGGALRPALTPS"
     gene            1251..2738
                     /locus_tag="PKN55_12545"
     CDS             1251..2738
                     /locus_tag="PKN55_12545"
                     /inference="COORDINATES: protein motif:HMM:NF012573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HNX72119.1"
                     /translation="MPVNISSSVSYTLGAAEDNLTLTGTANINGVGNALVNIINGNSG
                     ANLIKGLGGADTLYGYGGNDTLYAGDVGATGDVNTDLLYGGTGADVLYADGNDYLYGE
                     AGTDTLHGEFGANNRLAGGTENDIYYVSANGSTITELAAAGSDTVYSSDSIALNGAIL
                     VAGGTLVSGDVENLTLTGAANIDGVGNGLGNMILGNAGNNVLSGGLGIDTISAGDGND
                     TLYGVGAGAGVDAVTDNLDGGLGNDTLYGNGSDFLSGGLGDDVYLLIGGQNRVTDSAG
                     FDVVKTESSFTFGYTTDPAVSWGSGPIEVIELTGSLNTFANGEAGANTLLGNSGANTL
                     DGRGGNDILDGRDGDDVLKIGSDTGSVGGATNMTGGAGADTFWTGKGYRGDHSSGANQ
                     LQINDFTSGVDHIRFGIATTATAPTTLDTTAVYGGDTLAMVLDRAASGSGSPTAPTIS
                     QFVFGADTYLVLDLSTATTFSTGDLAIKLTGTPGVALSDLVFNLV"
     gene            2911..>3563
                     /locus_tag="PKN55_12550"
     CDS             2911..>3563
                     /locus_tag="PKN55_12550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008953676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HNX72120.1"
                     /translation="MRQDKLTTKFQQALADAQSLALANDHAYIEPQHLLAAMLDDADS
                     GIGSLLARAGVNAPRLRQQLAAAIERLPKVQGAGGEVNVSRELINLLNLTDKAAIKRG
                     DQFIACELFLVALTEDKGETGRLFKEAGATPAALNAAIDAVRGGEQVGSQDAEGKREA
                     LAKYTLDLTARAREGKLDPVIGRDDEIRRAIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     "
BASE COUNT          768 a         1052 c          972 g          771 t
ORIGIN      
        1 ctgttaatcc gcaggtccct ggttcgagcc caggtcgagg agccagattc aaaaaagccc
       61 gatgtcttca cgacatcggg ctttttttcg ttttcatgac ggaacttctg ctgaaacagc
      121 cagtcataca ctctacaact tgcgcgtttc aggcccagcg gataagccgc cgccgcgcca
      181 ggttgctgca gacaggaggc cgtgatgcaa ctgacttccc gccagatttc cccccgcttg
      241 cgccgcatgc tgcgccaatg ctggcaacgc ctgagcgaac gcgatcttga ccagatcgtc
      301 aatcgcaaaa gcctgctggc cgcgctgcga cgccgctata cccgccccgt ggtggagatc
      361 gaacaagaaa ttgaccaatt ttttcgcacg gtgtttgccg gaggcgcctt gcgcccggct
      421 ctgacgccat cctgatgcac atggggcggg ttgccccacc cgccttgtgg ctgttttctc
      481 cgggttgttc ccacatcctg ttcgcgcctg ccttgtgcag gcgttttttt tgatgccaga
      541 cttggcgcca aaccagcacc tggcaatgct aaataaatat tttataaatg aatttcataa
      601 aaatagctta tcagatcacc tgatctgacc aagaaccacg taaaaaaatt tcctagtgtt
      661 gttgtatcgc cttctcgtac tacttgctct gtttgcacac gcccagcctg ggttgctgca
      721 cagcattctg taatatttac tcagtaaatc tacttaaaca catctggcca tttgcatttc
      781 acgccttact caatgacgat ccagcgctat ggcagtttgc actcaagcag cgtatttatt
      841 tgttagtcag acagcgtggc ggctcgcctt gttaccccac cattctttgc ctttggtatt
      901 ttttgtccgg ccgcgttttt gccaaagcac atgacaagct atgccgcctc tggctgcaat
      961 gcgccactct gtgtaaaaat ccatacaata tactgttcat aaagaacaaa ttaaacaatc
     1021 gattacctta cgcaatttat ttgtatgtaa ttttttgttg acattttaat ttttaaaaac
     1081 agatctatag tagaaatact taggcacttt tacctaaaaa gaattttctg tcgtcccgcg
     1141 tgaatttttt gtgcagcatt tgatctccac caagatttcg tatagcgtat gccgtgctgg
     1201 tgaaaaatcc ccagtcattt ttcatttcat tactctcagc aggaggcatc atgcccgtca
     1261 atatcagctc cagcgtcagc tacacacttg gcgccgcaga agacaacctt accctgaccg
     1321 gaacagccaa catcaacggc gttggcaatg cactggtcaa catcatcaac ggcaattcgg
     1381 gggcgaacct gattaagggc ctgggtggcg ccgatacgct gtatggctat ggcggcaacg
     1441 acaccctgta cgctggcgac gtgggcgcca ctggcgacgt caacaccgat ttgctctatg
     1501 gcggcacagg cgcagacgtg ctgtatgccg atggcaacga ttacctgtac ggcgaagccg
     1561 gcacagatac cctgcatggt gaatttggcg ccaacaatcg tctggcaggc ggcacggaaa
     1621 acgacattta ctatgtgtcg gcgaacggca gcactatcac cgaactggca gccgcaggct
     1681 cggatacggt gtacagcagt gattcgattg cgctgaacgg cgccatcctg gttgctggcg
     1741 gcacgctggt cagcggcgat gtggaaaacc tcacgctgac cggcgccgcc aatattgacg
     1801 gcgtgggcaa cggcctgggc aatatgatct tgggcaatgc gggcaataat gtgctgtctg
     1861 gcggccttgg cattgacacc atcagcgctg gcgatggcaa tgacacactg tatggcgtag
     1921 gcgcgggcgc cggcgtggat gcggtgaccg acaatctgga cggcgggctg ggcaacgaca
     1981 cgctgtatgg caatggttcg gatttcttga gcggcggtct gggtgatgat gtttatctac
     2041 tgatcggcgg ccaaaaccgg gtgactgaca gcgctggctt tgatgtggtg aaaaccgagt
     2101 cgtcgttcac ctttggctat actactgacc cggccgtctc ctggggcagc ggcccgattg
     2161 aagtgattga actgacgggc tcgttgaaca ccttcgccaa tggcgaagcc ggcgccaaca
     2221 ccctgttggg caatagcggc gccaacacgc tggatggccg cggcggcaat gacattctgg
     2281 atggtcgcga tggcgacgat gtgctgaaaa ttggctcgga cacgggcagc gttggcggcg
     2341 ccaccaatat gaccggcggc gccggcgccg acaccttctg gaccggcaag ggctatcgcg
     2401 gcgaccacag cagcggcgcc aaccaattgc agatcaacga tttcacctcc ggtgtggatc
     2461 acatccgctt tggcatcgcc actaccgcca ccgcgccgac cacgctggac accaccgccg
     2521 tgtacggcgg cgacaccctg gccatggtgc tggaccgcgc tgcatccggc tcgggcagcc
     2581 cgaccgcgcc aaccatcagc cagtttgtgt ttggcgccga cacctatctg gtgctggacc
     2641 tgagcacagc caccaccttc tctaccggcg atctggccat caagctcacc ggcacgccgg
     2701 gcgtggcttt gtctgatctg gtgtttaatc tggtgtgatg gattaagagc aagctgattc
     2761 acgcagacca caccttttgc agcggcggcc aggaccacac tggccgccgc tgctgcttca
     2821 ccctatgcaa tttatggttt ttggggcttg aaaagtcctc cctgcccctc aactacggtc
     2881 ttgacaaaac tttctcgagg tgcacgaacc atgcgtcaag acaaactcac caccaaattc
     2941 caacaagctc tggccgatgc gcaaagcctg gcgctggcca atgatcacgc ctatatcgaa
     3001 ccgcagcact tgctggcggc catgctggac gatgccgatt ccggcattgg cagcctgttg
     3061 gcgcgcgccg gcgtcaacgc cccgcgcctg cgtcagcaat tggccgctgc gattgagcgc
     3121 ctgcccaaag tgcaaggcgc tggcggcgaa gtgaatgtgt cgcgcgaact gatcaatctg
     3181 ctcaatctca ccgacaaggc cgccatcaag cgcggcgacc aattcattgc ctgcgagctg
     3241 ttcctggtgg cgctcaccga ggacaagggc gaaaccggcc gtctgttcaa agaagccggc
     3301 gccacgccag cggcgctgaa cgccgccatc gacgccgtgc gcggcggcga gcaagtgggc
     3361 agccaggacg ccgaaggcaa gcgcgaggcg ctggccaaat acaccctcga cctcaccgcc
     3421 cgcgcacgtg aaggcaagct cgacccggtg attggccgcg acgatgaaat ccgccgcgcc
     3481 atccaggtgc tgcaacgccg caccaagaac aacccggtgc tgattggcga accgggcgtg
     3541 ggcaagacgg cgattgtcga agg
//
LOCUS       DAOPYX010000306         3549 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_35270_length_3549_cov_9.311677, whole genome shotgun sequence.
ACCESSION   DAOPYX010000306 DAOPYX010000000
VERSION     DAOPYX010000306.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 3549)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3549)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3549
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35270_length_3549_cov_9.311677"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(253..684)
                     /locus_tag="PKN55_12555"
     CDS             complement(253..684)
                     /locus_tag="PKN55_12555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX72121.1"
                     /translation="MKNMKKWIAMCAMLGAAAQTHAVVGSWTKQTAHYPIYFSGGYAE
                     MPFSSPTSMPTLTSFVTSVSYQWTGYGNGNTSRVVQICYSAKYSATIGRCHNISDRQQ
                     ATIHNFDGLDASGKFWIRHQIWGGTYPAISSALDTVTVMHQ"
     gene            complement(983..2251)
                     /locus_tag="PKN55_12560"
     CDS             complement(983..2251)
                     /locus_tag="PKN55_12560"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HNX72122.1"
                     /translation="MMKMPEWWRVRPGKRTLAALLVLILLAMAIYGGWRASGAADVGP
                     AGQTWQVHREDVDQQLALVGVVESARVIQLTAPFDGVVTEKRFQHNQLVKAGDALLHI
                     DASELAQQRAEAEVAYLKAQEQRRLFDHLDNVPDISRARRNLALAQAAWSNARKKAQE
                     TALLFDKGIVSRNERDDSEEQARQQAALAQSATEELASAQERFNAVQKQVAEVNLAAA
                     QHKYQALLHAEQNNPLTAPISGVALVSQPAQSGQTPVERGSRVTRGQPLLNLIDPANL
                     VVAFKVDEILVRRLQAGQEVQFYSDVLGQSLRGSVSFIAAQASPVLSAAKSAEFEVRA
                     ELAHEQTRQQAALIGSTGKLSLALPLWRHAIAIPVVFLQDSAQGPQVYVRLPDGQVRP
                     RSVQVGETQDGKVEIRAGLAEGEMLMTPPL"
     gene            complement(2235..>3549)
                     /locus_tag="PKN55_12565"
     CDS             complement(2235..>3549)
                     /locus_tag="PKN55_12565"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNX72123.1"
                     /translation="IQAAYLGRDTQWFDLYVAEGKFIPKTTLSGAYKTLSGAGGRARQ
                     IDGGVTSSLALPTGGTLSLSTARSLLPEQGQRSTLSSLTLTQPLLKNAGWAVAMSSIV
                     QARLNDASSRIGLQNTVAQTVAQIIYSYRNLLRAQEQIKIADDSLTRARRFLEMNQAL
                     AQAGRIPPVDVIQAEADVANQALALEENHNQLDSQRLELLNLLSLDLDTALTAQADAW
                     GLAVRLATPEQVDALIEQHPDYWAARLSLARARQRVLLANNQQLWDVALVAGLTQAQG
                     GANQGEAKKVDKYLGAQLSIPLFDRSIEQARVSAENDLKTEKIQLAQTRQTLRGKILN
                     AMRNVQARARQAELADKAAELTRQKLQAEQEKLKYGRSSSFQVILFENDLRAAENNRL
                     NARLAYLNSQVEWLLATGKLLDAWQAPLISHADVQTLLEADDEDA"
BASE COUNT          738 a         1022 c          961 g          828 t
ORIGIN      
        1 gacggtatac aagatttttg gctcaggcgc ggaagggtct ttgatccgcc acaaacgttc
       61 cagcatcagg gcatgccctc tggattgact ggcggtctgg ctgctggatg tggctaccgt
      121 ggcagttaag gtggcgatgg aagtcgttgc taaactgcta cttacgctgg tggtcatgat
      181 attctcccta aattcatcat ctacattttt gcgacaggga atcaaatatt tccctatcgc
      241 agagtcaaag tgtcattgat gcatgaccgt tacagtgtct aatgcactag agatggctgg
      301 ataagtccct ccccaaattt ggtgccggat ccagaatttc cctgaagcat ccagtccatc
      361 aaaattatga atagtagctt gctgcctgtc agagatatta tggcatctcc cgatagtggc
      421 cgaatatttg gccgaataac aaatttgcac aaccctggat gtattgccat ttccataccc
      481 cgtccactga tacgacacgg atgtcacaaa tgaagtcagt gtcggcatcg aagttggaga
      541 actgaagggc atctcagcat accctccact aaagtagatg gggtaatgtg ctgtttgttt
      601 tgtccacgac cccaccacgg catgagtttg tgctgctgcg ccgagcatgg cgcacattgc
      661 tatccatttt ttcatgttct tcatgatgag tccttttggt ctgtcaagat tggaacctct
      721 ctcatgttca gcatgaaaga ggcctgatga ctatcctaaa catgcccaac tgatgccgtc
      781 aattttcagt atagccatat gttgttttct tgccaaattc atgagcaaaa taggtagtta
      841 tactgatttt gtactgtgcc acaccaaacc aaaccaagta aaaatactta atattgctgc
      901 accaggctta aagcccataa aaaaatcatc ggcatcatta atccaacaaa caggctattc
      961 gtcatttctt ggatcgggga attcaaagcg gcggcgtcat cagcatttca ccctctgcca
     1021 gcccggcgcg aatttccacc ttgccatcct gtgtctcgcc gacctgcacg ctgcgcgggc
     1081 gcacctgccc atccggaaga cgtacataca cctgcgggcc ttgtgcgctg tcctgcaaaa
     1141 acaccacagg aatagcgatg gcgtggcgcc acagaggcaa ggccaggctc agtttgccgg
     1201 tagagccaat caatgcggcc tgttggcggg tctgttcatg cgccagttca gcacgcacct
     1261 caaattcggc gcttttggcg gctgagagca ctggcgaggc ctgggcggcg atgaacgaca
     1321 ccgagccacg caggctctgg cccagcacat cgctataaaa ctgcacttct tgcccagctt
     1381 gcagacgacg caccaatatc tcgtccactt taaacgccac caccaaattg gccgggtcga
     1441 tcagattgag caaaggctgg ccgcgcgtca cccggctgcc acgctcgact ggcgtttgtc
     1501 cgctttgggc gggttgactg accaaggcca cgccgctgat cggggctgtc agtgggttgt
     1561 tttgttcggc gtgcagcaag gcttgatatt tatgctgggc agcggccaga ttgacctcag
     1621 ccacctgctt ttgcaccgca ttgaagcgct cttgcgctga agccagttct tctgtggcgg
     1681 attgcgccaa cgccgcctgc tggcgagcct gttcttcgct gtcgtcgcgc tcattgcgcg
     1741 agacaatgcc cttatcaaac aacaaggcag tttcctgcgc ctttttgcgg gcattggacc
     1801 acgcggcctg cgccaaggcc agattgcgcc gggcgcggct gatgtcgggc acattgtcca
     1861 ggtgatcaaa caagcggcgc tgttcctggg ctttcaggta ggccacttcg gcttcagcgc
     1921 gttgctgggc caattcgctg gcgtcgatgt gcagcaaggc gtcgccggcc ttcaccagtt
     1981 gattatgctg aaagcgtttt tctgtcacca cgccatcaaa tggcgcggtc agctgaatca
     2041 cacgggcgga ctccaccacc cccaccagcg ccagttgctg atcaacatct tcgcggtgaa
     2101 cttgccaggt ttgcccagca gggccgacat ccgccgcgcc actggcgcgc cagccgccat
     2161 atatggccat ggccagcaag atcagaacga gcaaagcggc cagggtgcgc ttgcctggac
     2221 ggacgcgcca ccattcaggc atcttcatca tcggcctcca gcagggtttg cacatcggca
     2281 tggctgatca gcggcgcctg ccaggcgtcc agcagtttgc ctgtggccag cagccattct
     2341 acttggctgt tcagataagc aaggcgcgca ttcaagcgat tgttttctgc tgcgcgcagg
     2401 tcgttttcaa acaagatcac ctgaaaactg ctggaacggc cgtatttgag tttttcctgc
     2461 tcagcctgga gtttttgccg ggtgagctca gcggctttgt cggccaattc cgcctggcgc
     2521 gctctggctt gcacattgcg catggcgttt agaattttgc cgcgcagggt ttgccgggtt
     2581 tgcgcgagtt gtattttttc ggttttcaga tcgttttctg cgctgacgcg ggcttgttca
     2641 atgctgcggt caaacagagg aatgctgagc tgggcgccaa ggtatttgtc tacttttttc
     2701 gcctccccct gattggcgcc cccctgggcc tgagtgagcc ccgctaccag cgccacatcc
     2761 cataactgct gattattcgc taacagcaca cgctgccggg cgcgcgccag cgacaaacgg
     2821 gcagcccaat aatcggggtg ctgctcaatc agggcgtcca cttgttcggg ggtggccagg
     2881 cgaaccgcca ggccccaggc atcagcttgg gcagtcaatg cggtgtccaa gtccagcgac
     2941 aaaagattca gcagctccag ccgctggcta tccagctggt tgtggttttc ctccagcgcc
     3001 aaggcctgat tggcgacatc ggcctcagcc tgaatcacgt ccacgggcgg gattcgtccc
     3061 gcctgggcca aggcctgatt catctccaga aagcgccgcg cgcgcgtcag gctgtcatcg
     3121 gcaattttga tctgctcttg cgcacgcagc agattgcgat agctatagat gatttgcgcc
     3181 acggtctggg ccacggtgtt ttgcaggcca attcgactgg acgcatcatt cagccgcgcc
     3241 tggacaatag acgacatggc caccgcccaa ccggcatttt tcaacaaagg ctgtgtcagc
     3301 gtcaggctgg acaaagtgct gcgctggcct tgttctggca acagcgaacg cgccgtggac
     3361 agcgataagg ttccgccggt gggcaaagcc agcgacgatg tgaccccgcc atcaatctgg
     3421 cgcgcacgcc cccctgcgcc ggacaaggtt ttataggcgc cggacagggt agttttggga
     3481 atgaacttgc cttcggccac atacagatca aaccactggg tatcacgccc caggtaagcc
     3541 gcctgtatg
//
LOCUS       DAOPYX010000307         3541 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_35413_length_3541_cov_5.372920, whole genome shotgun sequence.
ACCESSION   DAOPYX010000307 DAOPYX010000000
VERSION     DAOPYX010000307.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 3541)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3541)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3541
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35413_length_3541_cov_5.372920"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            122..1360
                     /gene="yjjJ"
                     /locus_tag="PKN55_12570"
     CDS             122..1360
                     /gene="yjjJ"
                     /locus_tag="PKN55_12570"
                     /inference="COORDINATES: protein motif:HMM:NF007297.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HipA family toxin
                     YjjJ"
                     /protein_id="HNX72124.1"
                     /translation="MPDADLLALLRHGPLDAPSLCQRLAISQPTLSRRIAEAGDAIVR
                     IGRARATRYLARRAVRAQHSFVLYQVDASGQAAPWGELIPVHPGFVWRANNGVSNWSE
                     GLPWCLQDMRPQGFLGRAWGRARASALALPEDVQDWSDEHVLIALAAGEADAPGNLLI
                     GQASLAAWRAHAPLSLRREQRAEHYPRLAAQVMAGEPIGSSAGGEQPKFAVRVDGRDV
                     LVKFSAASNNAVSQRWRDLLRAEHLALRTLAEHGLAAADSEIFEHDGQCFLQLTRFDR
                     TPAGGRLALTSLAALDAEFLGLGAAVWPQYTAALLAQHRITPRAHHDACRLYAFGVLI
                     GNTDMHTGNLSFLHNDPHPLELAPAYDMLPMALAPNRQGDMRDSIPRPVQNSWVTPEI
                     WAEMEAVAREYWARLGWGKA"
     gene            1757..2038
                     /locus_tag="PKN55_12575"
     CDS             1757..2038
                     /locus_tag="PKN55_12575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013124611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HNX72125.1"
                     /translation="MASASTMIHVRVDEHLKTQATETLAAMGLTVSDAVRVFLTRVVA
                     DQELPFALKAPNAASRAAIAEANELIQTRQARFASADALLDELEKNSRQ"
     gene            2010..2339
                     /locus_tag="PKN55_12580"
     CDS             2010..2339
                     /locus_tag="PKN55_12580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019920430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system YafQ family
                     toxin"
                     /protein_id="HNX72126.1"
                     /translation="MSSKKTAASKRTSLPRASDYTKAFLKDWQRLSHAGRYDMNRLKE
                     VMLLLIANQGSLPPEWLDHPLTGDWHGHRECHVGGDFLLIYTLDDSNKNGMVVFVRCG
                     THAELFA"
     gene            2531..3475
                     /locus_tag="PKN55_12585"
     CDS             2531..3475
                     /locus_tag="PKN55_12585"
                     /inference="COORDINATES: protein motif:HMM:NF022633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2971 domain-containing protein"
                     /protein_id="HNX72127.1"
                     /translation="MQDWKEIYVQHKFPLYLFSQTDLADVLKKRNWPSRLYKYRSFSD
                     FHTNALSDFSVWLSTPIGFNDTRDCSYNINPHRQMNFILSKSKESILELAEILLLDER
                     QKASLCNSKAPHTDIMNIIRQTMPGAAADELSELYNYLFEESILSEFNKKLKQRFHVC
                     SLSAKRDSPLMWAHYANEHKGFCVEYNVEDLPGNSNEFFDMIHPVFYTDNIFDATSYL
                     IATTPELKNPEFLQQAALIKTKDWAYEDEWRLMVESSGEEGRLLRFEKPSAIYLGERT
                     ETDKIEWMLKFCTSHHIMCFLMKTDPKSVGMAVGRRLT"
BASE COUNT          799 a          992 c          968 g          782 t
ORIGIN      
        1 cgggtagcgg gctaggcggc caggctgagg cgggggtgga gtatctattc acgcctggcg
       61 gcaggaaaac accaagttgt tgatatataa tcagactttg ccaaaagaat ctattcacgc
      121 catgcccgac gccgatttgc tcgccctgct gcgccacggc ccgctggatg cccccagcct
      181 gtgccagcgc ctggccatca gccagcccac cctgtcgcgg cgcattgctg aggctggcga
      241 cgccattgtg cgcatcggcc gggcgcgcgc cacccggtat ctggcccgtc gcgccgtgcg
      301 tgcccagcac agctttgtgc tgtaccaagt agacgccagc gggcaggctg cgccgtgggg
      361 tgagttgatt ccggtgcatc cgggttttgt ctggcgcgcc aacaacggcg tcagcaactg
      421 gagtgaaggc ctgccatggt gcttgcagga catgcgcccg caaggttttc tgggccgcgc
      481 ctggggccgc gcgcgcgcca gcgccctggc cttgccggaa gacgtgcagg actggagcga
      541 cgagcatgtg ctgattgccc tggcggccgg cgaggctgac gcaccgggca atctcttgat
      601 tggccaagcc agcctggccg cctggcgcgc ccatgcgccg ctgtcactgc gccgcgagca
      661 gcgcgccgag cactatccgc gtctggcggc gcaggtgatg gccggcgagc cgattggctc
      721 cagcgcgggc ggcgaacaac ccaaattcgc cgtgcgggtg gacggccggg atgtgctggt
      781 gaaattcagc gccgccagca acaacgccgt cagccagcgc tggcgcgacc tgttgcgagc
      841 cgaacacctg gccttgcgca cactggcgga acacggcctg gccgctgccg acagcgaaat
      901 attcgaacac gacggccagt gctttttaca actcacccgg tttgaccgca ccccagccgg
      961 cggccgcctg gcgctgacct cgctggcggc gctggacgcc gagtttcttg gcctgggcgc
     1021 cgccgtctgg ccgcaataca ccgccgccct gctggcgcaa caccgcatca ccccccgcgc
     1081 ccaccacgac gcctgccggc tgtacgcctt tggcgtgctg attggcaaca ccgacatgca
     1141 caccggtaac ctgtcgtttc tgcacaacga cccacacccg ctagaactcg cccccgccta
     1201 cgacatgctg cccatggccc tggcccccaa ccgccaaggc gatatgcgcg atagcatccc
     1261 gcgccccgtg caaaacagtt gggtgacgcc ggaaatctgg gcggagatgg aagcggtggc
     1321 gcgggagtat tgggcgagat tggggtgggg gaaagcgtag catcaacaaa gatgaacaac
     1381 ttacccaaga gacgccaaat gtcgagccaa gcccccacta ccccgcaaca agaaagccac
     1441 agcacaaggc gtgacgccgc gcatcctggt cgaatcggct ggtttgacgg ctattgcgcc
     1501 gaggacgacg acgatgcatg ggcggggttc tcacctgagg aagaaaagga cggtgagtgg
     1561 tgggagtcgc gtctattcac tgattaggca tgtgcgcgct attggcttga tcacaaatgg
     1621 gtttctcaga aagtcagcca tgcacaccgc gccacaatgc ggacacgctc cctggttttt
     1681 tcacccaggt cattgccttg cgctggcagt ggtttgcgat acaatgaatc tcaaatcatc
     1741 gccaggaaac cccttcatgg cctctgcctc caccatgatc cacgtccgtg tggatgaaca
     1801 cctcaaaacg caagccaccg aaacgctggc cgccatgggc ctgaccgtct cggatgcggt
     1861 gcgtgtattt ctgacccgcg tggtggctga ccaggaactg ccatttgcgc tgaaagcccc
     1921 caacgccgcc agccgcgccg ccatcgccga agctaacgaa ctgatccaaa cccgccaagc
     1981 gcgctttgcc agcgcggatg cgttgcttga tgagctcgaa aaaaacagcc gccagtaaac
     2041 ggacatctct gccgagggca tccgactaca ccaaagcttt tctcaaggat tggcagcggt
     2101 tgtcgcatgc aggtaggtat gatatgaatc ggctgaaaga ggtcatgtta ctgcttatcg
     2161 caaatcaggg atctttgcca cccgaatggt tagaccatcc gctgactggc gattggcatg
     2221 ggcaccgtga atgccatgtt ggtggtgact tcttgctgat ctatacactc gacgacagca
     2281 ataaaaatgg gatggtggtg tttgtgcgct gcggcactca tgcagagttg tttgcgtaat
     2341 ggtgcatatg ctttttgggt tatttgctgg ggagggggtt ttatcccacg aaggaggtgg
     2401 ctcaagtctt gtaaagcttg caagactttg cttttgggga tatttcgatt tttctggata
     2461 gatccgtaca ccctttgttt ttatttttta tcccaaatag caatttcatg gcaaactata
     2521 aggagtgacg atgcaggatt ggaaagagat atacgtacag cacaaatttc ctttgtatct
     2581 gttctcccaa acagatcttg ctgatgtgct taagaaacga aattggccta gccggttata
     2641 caaatacaga tcattttccg atttccatac taatgccctt tctgattttt ccgtatggct
     2701 ctcgacccca ataggcttta atgatacacg tgattgctct tacaatataa accctcatag
     2761 gcagatgaat tttatcttgt caaaaagcaa ggaaagcatt ctggagttgg ctgagatttt
     2821 attgttagat gaaaggcaaa aggcatccct ttgtaattcg aaagccccac acactgacat
     2881 aatgaacatt ataaggcaaa ctatgccagg cgctgctgct gatgaacttt ccgagctcta
     2941 caattatctt tttgaagaaa gcatattatc tgagtttaat aaaaaattaa agcaacgctt
     3001 tcatgtttgt tctttgtcgg ctaagcgaga ctcgccgctc atgtgggctc actatgcaaa
     3061 tgagcacaaa ggcttctgtg ttgagtataa cgtggaggac ttgcctggca atagtaatga
     3121 attttttgac atgatacatc ccgtttttta caccgataat atatttgatg ccacctcata
     3181 tctcattgcc actaccccag aactgaaaaa ccctgaattt cttcagcagg ccgctttaat
     3241 aaaaaccaaa gattgggctt atgaagacga gtggagatta atggtagagt cttcaggtga
     3301 agaggggcgg cttcttaggt ttgaaaagcc atccgccatt tacctggggg agcgcacaga
     3361 aacggataag attgaatgga tgctaaaatt ttgtacatct caccatatca tgtgctttct
     3421 tatgaaaaca gatcctaaga gtgttggaat ggctgtaggg cgtagattaa cgtaaagaaa
     3481 ttagccccac cctctccccc aacccactct gccgcctcgt ctccgtcccc gccgccagcg
     3541 c
//
LOCUS       DAOPYX010000308         3515 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_35787_length_3515_cov_9.273121, whole genome shotgun sequence.
ACCESSION   DAOPYX010000308 DAOPYX010000000
VERSION     DAOPYX010000308.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 3515)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3515)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3515
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35787_length_3515_cov_9.273121"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(16..1275)
                     /locus_tag="PKN55_12590"
     CDS             complement(16..1275)
                     /locus_tag="PKN55_12590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX72128.1"
                     /translation="MTCLPLRWRRAACALAALLAATPSQADDPAWHGVLRTYWQGGRA
                     ANAGPLAAADAAGLAISPPSTLNSEAQLQGALALGGAQLRVEALARQRRRSGEGEQGR
                     MQFNEAQLSGGSGPWQWTVGKKVLSWDVGQGFRPNDLVQQETRRSLLNLPLEGRSMLL
                     GEYFSAERAWSLAAVNPTARRSATGGSEPALAARVYQRDGDADWHGFARLGAHTGASV
                     GAAAAWVVNESLELHASARYLRHHDTLRHSADSPPLSASNPWLNQRQGSARQWLIGGS
                     WTTAEQISLLLEAWRDGAAPSAADWRGWAARNAELSRYAAAGAPAMAVAGNQAWQAQM
                     FPTSGNLRRDSLFARLSWTHDGWQPTLDALYHPQDGGLMLTAALSWQGDRLKLETGLR
                     RHAGPASALIRQLPQRSQAYAWLEWAY"
     gene            complement(1281..2018)
                     /locus_tag="PKN55_12595"
     CDS             complement(1281..2018)
                     /locus_tag="PKN55_12595"
                     /inference="COORDINATES: protein motif:HMM:NF028441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HNX72129.1"
                     /translation="MKHAACLLALCAIAPAWGQDLDTLLRDADRYRMSADNLQVETLV
                     QTYSASGELDKERRYTVFAQAGRQSLVLMQSPAEQGQKVLMLGDDFWLLMPNSQRPLR
                     ITPMQKLLGDASTGDIATLSWSGDYRAELVGEEPCQPNEQARQCLHLSLHATRKGVTY
                     SRIELWLGKTRHEPVRADLYVQSDKLAKQARFTLDKARSTVTDMILTDQLNKQKETRV
                     SYQSRKPRSIPDTWLNPMFLVRNPSLD"
     gene            complement(2051..3274)
                     /locus_tag="PKN55_12600"
     CDS             complement(2051..3274)
                     /locus_tag="PKN55_12600"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HNX72130.1"
                     /translation="MRALLTWLKFAWHNTLRNRRRSLVTVSIVALGAAGMLLAGGFAL
                     YTYESLAEASARDSGHLVLGAPAQFDRDEDVPLQHGLSGVQKLRATLLADPAVRHVLP
                     RVAFTGLISNGDKSTVMLAAGVDPDGEFTVKGPFLTVTAGKTLAADAAQPQVMLGADL
                     ARNLKARPGDSLTLLASTTEGAMNAVDVVVAGLFTTGVPEVDKRMVYTSIATAQQLLV
                     TDKVSTLGVFLDRMEATEPARQRLAAQLPQLAVQTWEEQAAFYRAVRNLYNRIFGALG
                     LIIAVIVVSVVANAMAMAIIERTREIGALRALGTLPGQLLCTLGLEGALLGGAGALLG
                     AALALAISALLHVFPMDMPPPPGRSVGYPLLVTVDASMYAVVMSLMLLLTLTASLLVA
                     RKTVRLPVVDALAHT"
     gene            complement(3259..>3515)
                     /locus_tag="PKN55_12605"
     CDS             complement(3259..>3515)
                     /locus_tag="PKN55_12605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012837162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNX72131.1"
                     /translation="ALSGGQRQRVAIARALIKRPSLVIADEPTASLDSHTAEQVLALM
                     RERGRAEGAAFVIATHDNRLTARCDRVVNLLDGRIQCAPC"
BASE COUNT          495 a         1143 c         1262 g          615 t
ORIGIN      
        1 cacgtcccgc attcttcaat acgcccactc cagccaagca tacgcctggc tgcgttgcgg
       61 cagttgccgg atcagcgcgc tggcggggcc ggcgtggcgg cgcaggccgg tttccagttt
      121 caggcggtcg ccttgccagc tgagtgcggc ggtgagcatc agcccgccgt cctgcgggtg
      181 atacagcgcg tccagcgtgg gttgccagcc gtcgtgcgtc cagcttagcc gggcaaacag
      241 gctgtcgcgg cgcagattgc ccgaggtggg gaacatctgc gcttgccagg cttggttgcc
      301 ggccaccgcc atggctggcg cgccggcagc ggcgtagcgg ctcagttcgg cattgcgcgc
      361 ggcccagccg cgccagtcgg cggcggacgg cgcggcgccg tcgcgccagg cttccagcag
      421 caggctgatt tgctcggcgg tggtccagct gccgccgatc agccattgcc gggcgctgcc
      481 ttgccgctgg ttcagccagg ggttgctggc gcttaatggt gggctgtcgg cgctgtggcg
      541 cagggtgtcg tggtggcgca ggtagcgcgc cgaggcgtgc agttccagcg attcattcac
      601 cacccaggcg gcggccgcgc ccacgctggc gccagtgtgg gcgcccaggc gggcaaagcc
      661 gtgccagtcg gcgtcgccat cgcgctggta cacccgcgcc gccagcgccg gctcgctgcc
      721 gcccgtggcg ctgcgccggg cggtggggtt gaccgccgcc agcgaccagg cgcgctcggc
      781 gctgaaatat tcacccagca gcatgctgcg cccttccagc ggcagattca gcaggctgcg
      841 ccgggtttct tgttgcacca ggtcgttggg gcgaaagccc tggccgacat cccagctgag
      901 cacttttttg cccacggtcc attgccaggg gccgctgccg ccgctgagtt gggcttcgtt
      961 gaattgcatg cggccttgct cgccctcgcc gctgcgccgt cgctggcgcg ccagcgcctc
     1021 cacccgcagt tgcgcgccgc ccagcgccag cgcgccttgc agctgggctt cgctgttgag
     1081 cgtgctgggc gggctgatgg ccaggccggc ggcgtcggcg gcggccagcg ggccggcatt
     1141 ggcggcgcgg ccgccttgcc aataggtgcg cagcacgcca tgccaggcgg ggtcatcggc
     1201 ctggctgggc gtggcggcca gcagcgcggc cagcgcacag gcggcgcggc gccaccgcag
     1261 gggtaagcag gtcatcgggc ttagtccagg ctggggttgc gcactaaaaa catcgggttc
     1321 agccaggtgt cgggaatgct gcgcggcttg cggctttggt agctcactcg ggtttctttt
     1381 tgtttgttca gttggtcggt caggatcatg tcggtgacgg tgctgcgcgc cttgtccagg
     1441 gtaaagcgcg cctgcttggc cagtttgtcg gattgcacat acagatcagc gcgcaccggt
     1501 tcgtggcggg ttttgcccag ccacagctcg atgcgcgagt aggtcacccc cttgcgtgtg
     1561 gcgtgcaggc tcaggtgcag gcattggcgg gcttgctcat tgggctggca gggttcttcg
     1621 cccaccagtt cggcgcggta gtcgccgctc cagctcagtg tggcgatgtc gccggtagag
     1681 gcgtcgccca gcagtttttg catcggcgtg atgcgcagcg ggcgctggct gttgggcatc
     1741 agcagccaaa agtcatcgcc cagcatcagc actttttggc cttgttcggc cgggctttgc
     1801 atcagcacca gcgattggcg gccggcctgg gcaaacacgg tgtagcggcg ttctttgtcc
     1861 agctcgccgc tggcgctgta ggtttgcacc agggtttcca cttgcaggtt gtcggcgctc
     1921 atgcggtagc ggtcggcgtc gcgcagcagg gtgtcgaggt cttgtcccca ggcgggggcg
     1981 atggcgcaga gcgccagcag gcaggcagcg tgtttcatgg cgggccttgg taaagagaga
     2041 gataaggtca tcaggtatgc gccagcgcat ccaccaccgg caggcgcacg gttttgcgcg
     2101 ccaccagcag cgaggcggtc aaggtcagca gcagcatcag gctcatcacc acggcgtaca
     2161 tgctggcgtc cacggtcacc aacagcgggt agccgacgct gcggccgggt gggggcggca
     2221 tgtccatcgg aaacacatgc aacagcgcgc tgatggccag cgccagcgct gcgcccagca
     2281 gggcgccggc gccgcccagc aaggcgcctt ccaggcccag tgtgcacagc agttggccgg
     2341 gcagggtgcc cagcgcgcgc agggcgccga tttcgcgggt gcgctcgatg atggccatgg
     2401 ccatggcgtt ggccaccacg gacaccacaa tcaccgcgat gatcaggccc agcgcgccaa
     2461 agatgcggtt atacagattg cgcaccgccc ggtagaacgc ggcttgttct tcccaggttt
     2521 gcaccgccag ttgcggcagt tgcgcggcca ggcgctggcg cgcgggctcg gtggcttcca
     2581 tgcggtcgag aaacacgccc agggtggaga ctttgtcggt caccagcagc tgctgggcgg
     2641 tggcgatgct ggtgtacacc atgcgcttgt ccacctccgg cacgccagtg gtgaacagcc
     2701 cggcgacgac gacgtccacc gcgttcatgg cgccttcggt ggtgctggcc agcagggtga
     2761 ggctgtcgcc ggggcgggct ttcaggttgc gcgccaggtc ggcgcccagc atcacttgcg
     2821 gctgggcggc gtcggcggcc agggttttgc cggcggtgac ggtcaaaaac ggccccttga
     2881 cggtgaattc gccgtccggg tccaccccgg cggccagcat caccgtggat ttgtcgccat
     2941 tgctgatcaa gccggtaaac gccacgcgcg gcagcacatg gcgcaccgcc gggtcggcca
     3001 atagcgtggc gcgcagtttt tgcacgccgc tcaagccgtg ttgcagcggc acgtcttcgt
     3061 cgcggtcaaa ctgcgcgggc gcgcccagca ccaggtggcc gctgtcgcgg gccgaggctt
     3121 cggccaggct ttcgtaggta tacagggcaa agccgcccgc caacagcatg ccggccgcgc
     3181 ccagcgccac aatcgacacc gtcaccagcg agcggcggcg gttgcgcagg gtgttgtgcc
     3241 aggcaaattt caaccaggtc agcaaggcgc gcattggatt ctcccgtcca gtaaattcac
     3301 cacccgatca cagcgggcgg tcagccggtt gtcgtgggtg gcgatgacaa aggcggcgcc
     3361 ttcggcgcgg ccgcgttcgc gcatcagcgc cagcacttgc tcggcggtgt gcgaatccag
     3421 gctggcggtg ggttcgtcgg caatcaccag gctggggcgc ttgatcagcg cgcgggcaat
     3481 cgccacccgc tggcgctggc cgccgctgag cgcat
//
LOCUS       DAOPYX010000309         3512 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_35836_length_3512_cov_12.261788, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000309 DAOPYX010000000
VERSION     DAOPYX010000309.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 3512)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3512)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3512
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35836_length_3512_cov_12.261788"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            110..373
                     /locus_tag="PKN55_12610"
     CDS             110..373
                     /locus_tag="PKN55_12610"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNX72132.1"
                     /translation="MRFAGQCVELWHWQLAPGVTYEAEPDPPGSQEMLYIISGELVLT
                     LDGLLRPLVTGTSIAFASDQPYRFHNPGPFMLRFSKNILLAAE"
     gene            637..903
                     /locus_tag="PKN55_12615"
     CDS             637..903
                     /locus_tag="PKN55_12615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX72133.1"
                     /translation="MSTYAVILPAEKIKQFAEQLPVEYSASTVYINGIFFLMSNKSAF
                     TLCKEFQASLASESIMVLGVSRNTAVLGVAHDKTSSLQKLVALA"
     gene            complement(983..1396)
                     /locus_tag="PKN55_12620"
     CDS             complement(983..1396)
                     /locus_tag="PKN55_12620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX72134.1"
                     /translation="MFLLLLISSTLVALGAALLTVRFFDAAILRILHTILGAELASAW
                     QRYLRFAIVVVGVSGGVRPLDLEKYIAPNRPGATAPELSLERWTLELYRSLIDSLQSI
                     AWMLLLFFIGAMIAYVLLRTVARRRSQVATLDDEA"
     gene            complement(1494..>3512)
                     /locus_tag="PKN55_12625"
     CDS             complement(1494..>3512)
                     /locus_tag="PKN55_12625"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014085677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008670 - 2,4-dienoyl-CoA reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0033543 - fatty acid beta-oxidation,
                     unsaturated, even number, reductase/isomerase pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HNX72135.1"
                     /translation="MSAYPHLLAPLDLGFTTLKNRVLMGSMHTGLEEAPGGFARMAAF
                     YAERARGGVGLMVTGGVAPNEAGRVMMHSAMLTNEHEAEQHRVVTDAVHAAGGKIALQ
                     ILHTGRYSYQPNPVAPSALQAPINPFRPVALSSEQVWQTIADFARCARLAQQAGYDGV
                     EVMGSEGYLINQFIAARTNQRDDEWGGSYQNRIRFALEILKAVREAVGPNFIIIYRLS
                     MLDLVEEGSSFDEIVELAQAVEAAGANLINTGIGWHEARIPTIATLVPRAGFAWVTQK
                     LMGKVGIPLITTNRINTPDVAEQVLAEGCADMVSMARPFLADPAFVAKAADNRADEIN
                     TCIGCNQACLDHIFAGKLASCLVNPLACHETERVITLAAQAKRVAVVGAGPAGLSCAV
                     TAAQRGHAVTLFDAASEIGGQFNVAKQIPGKDEFQETLRYFARQLELNQVTVKLGAWV
                     SADDLADFDEVVLATGIRPRLPDIPGIEHPKVLTYLDVLKHKKPVGQKVAIIGAGGIG
                     FDTAEYLSHSRDAAHGRDAFLREWGVDAELAARGGLCADGGQPPASPREIYLLQRKSS
                     KPGDGLGKTTGWIHRTALKNRQVRMLAGVNYLRIDDAGLHINVQGQVSVLPVDHVVIC
                     AGQEPCRELQAGVAARGVPVHLIGGADVAAELDAKRAILQGLETAMAL"
BASE COUNT          621 a         1109 c         1118 g          664 t
ORIGIN      
        1 tgttcgcaga tcaggaggct tcgggccagc tgttggggaa taaatcagtg cgtccctaag
       61 ggtctggcag gatgagcaca gtgatagcca tggcaccttg ctggagagcg tgcgctttgc
      121 tggccagtgc gttgaactat ggcattggca gctggctccc ggtgttacct atgaagccga
      181 gccggatccg ccgggcagtc aggaaatgct gtacatcatc agtggggaac tggtgctgac
      241 gctggatggc ttgctacgcc cgctggtgac cggcacgtca attgcttttg cttctgacca
      301 gccttaccgt tttcacaacc ctgggccgtt catgctgcgt ttcagcaaga atatcctgct
      361 ggctgccgag tgagaaatcc cccgtctgca ttggtcaagc gacggagaca ttcatgcaac
      421 atggttgtga aaaaatcggt ctgatggccg ataaatgcgt aaggtttgac ttacgacaac
      481 accgcctggg actaaattag caattctaat gcccactgca tgatgcccgc tgcgccagga
      541 tggcgcgccc caagggcgga tcaagccgct ttcgtcccta tcttcaaggg tgcgcaagcg
      601 gcgctttctc atgaacaccg tcaaggacag cacgccatgt ctacctacgc cgtgattttg
      661 cccgccgaaa aaatcaagca gttcgctgaa cagttgccgg tggagtactc cgcgtccacg
      721 gtgtacatca acggcatctt tttcctgatg tccaacaaat ctgcgttcac cctgtgcaaa
      781 gagttccagg cctcgctggc gtctgagtcg atcatggtgc tcggcgtgtc gcgcaacacc
      841 gcggtgctgg gcgttgccca cgataaaacg tccagcctgc aaaaactggt ggcgctggcc
      901 tgatctgatc ccggccccct gatgcgcctg cgcccccgtc tcagcctggc tgagcggggg
      961 cgcagtctgt tcccgcgtgt gcttacgctt cgtcgtccag cgtggccacc tggctgcggc
     1021 gtctggccac ggtgcgcagc agcacatagg caatcatcgc gccgataaaa aacagcagca
     1081 gcatccaggc aatgctttgc aggctgtcga tcagcgagcg gtacagctcc agcgtccagc
     1141 gctccagcga caattccggc gcggtggcgc cgggccggtt tggcgcgatg tatttttcca
     1201 gatccagcgg gcgcaccccg cccgacaccc ccaccaccac aatggcaaag cgcagataac
     1261 gctgccaggc gctggccagt tcggcgccca gaatggtgtg cagaatgcgc aggatggcgg
     1321 cgtcaaaaaa gcgcacggtc agcagcgcgg cgcccagcgc caccagggtg gagctgatca
     1381 gcaacaataa gaacatgagt gtcacccatg gcccgccatc tggctggcct gaaaacttgc
     1441 cggtcaaaaa aagcgcgccg atcggcgcgc catgatttta agccatgcgc gtttcaaagc
     1501 gccatcgcgg tttccagccc ttgcaggatg gcgcgcttgg cgtcgagctc agcggccacg
     1561 tccgcgccgc caatcaagtg caccggcacg ccccgcgccg ccacgccggc ttgcagctcg
     1621 cggcagggtt cctggccggc gcaaatcacc acatggtcca ccggcagcac gctgacctgg
     1681 ccttgcacat tgatgtgcag gccggcgtcg tcgatgcgca gataattcac cccggccagc
     1741 atgcgcacct ggcggttttt cagcgcggtg cggtgaatcc agccggtggt cttgcccagg
     1801 ccgtcgcccg gcttgctgct cttgcgttgc agcaggtaaa tctcgcgcgg gctggccggc
     1861 ggctggccgc catcggcgca caaaccgccg cgcgccgcca gctcggcgtc cacgccccat
     1921 tcgcgcagga aggcgtcgcg gccgtgtgcg gcgtcgcggc tgtggctgag gtattccgcc
     1981 gtgtcaaagc cgatgccgcc ggcgccgatg atggcgactt tctggcccac cggttttttg
     2041 tgcttgagca cgtccagata ggtcagcact ttcgggtgct caatgccggg gatgtccggc
     2101 aggcgtgggc ggatgccggt ggccagcacc acttcgtcaa aatcagccag atcatcggcg
     2161 ctgacccagg cgcccagttt gacggtgacc tgattcagtt ccagctggcg ggcaaagtag
     2221 cgcagggttt cctgaaactc gtccttgccg ggaatctgct tggcgacatt aaactggccg
     2281 ccgatttcgc tggcggcgtc aaacagcgtc accgcgtggc cgcgctgggc ggcggtgacc
     2341 gcgcaggaca aaccggccgg gccggcgccc accacggcca cgcgcttggc ttgcgccgcc
     2401 agggtgatca cccgctcggt ttcgtggcaa gccagcgggt tgaccaggca actggccagc
     2461 ttgccggcaa agatatggtc cagacaggcc tggttgcagc caatacaggt gttgatttcg
     2521 tcggcgcggt tatccgccgc cttggcgaca aaagccgggt cggccaggaa ggggcgcgcc
     2581 atcgacacca tatcggcgca gccctcggcc agcacctgct cggccacgtc cggggtgttg
     2641 atgcggttgg tggtgatcag cggaatgccc accttgccca tcagtttttg cgtcacccag
     2701 gcaaagccgg cgcgcggcac cagggtggcg atggtgggga tgcgcgcctc gtgccagcca
     2761 atgccggtgt tgatcaggtt ggcgccagcg gcttcgacgg cctgcgccag ctcgacgatt
     2821 tcgtcaaagc tgctgccttc ttccaccaga tccagcatcg acaagcggta aataataatg
     2881 aagttcggcc ccaccgcctc gcgcaccgcc ttgaggattt ccagggcaaa gcggatgcgg
     2941 ttttggtagc tgccgcccca ttcgtcgtcg cgctgattgg tgcgcgcggc gatgaattgg
     3001 ttgatcagat agccttccga gcccatcacc tcgacgccgt cgtagccggc ttgctgggcc
     3061 aggcgcgcgc agcgggcaaa gtcggcaatg gtttgccaca cttgctcgct gctcagcgcc
     3121 accgggcgga aggggttgat cggcgcttgc agcgccgacg gcgccaccgg gttgggctgg
     3181 taagagtaac gcccggtgtg caggatttgc agggcgattt tgccgcccgc ggcgtgcacg
     3241 gcgtcggtca ccacgcggtg ctgctcggct tcgtgctcgt tggtgagcat ggcgctgtgc
     3301 atcatcaccc gcccggcttc gttgggggcc acgccgccgg tgaccatcag gcccacgccg
     3361 ccgcgcgcgc gctcggcgta aaacgccgcc atgcgggcaa agccgccggg ggcttcttcc
     3421 agcccggtgt gcatcgagcc catcaggaca cggtttttca gcgtggtgaa gcccaggtca
     3481 agcggggcga gcaggtgcgg gtaggcggac at
//
LOCUS       DAOPYX010000310         3501 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_35995_length_3501_cov_9.597214, whole genome shotgun sequence.
ACCESSION   DAOPYX010000310 DAOPYX010000000
VERSION     DAOPYX010000310.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 3501)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3501)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3501
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35995_length_3501_cov_9.597214"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            227..1222
                     /locus_tag="PKN55_12630"
     CDS             227..1222
                     /locus_tag="PKN55_12630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008952387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HNX72136.1"
                     /translation="MELIAPAGNLAALKAAVLAGADAVYLGLKNATNARNFAGLNFTE
                     ADIAQGVAFARQHGRQVLFAINTFAQPGRAQEWRAAVDAACRLGADALILADPGVLAY
                     ARQRYPQQRLHLSVQGSATHADAIELMREQFGIQRVVLPRVLTVADIARLIEKTTVEV
                     EVFGFGSLCVMAEGRCLLSSFATGDSPNNRGVCSPAHAVRWQPRDGQMEARLNGLLID
                     RYAPDEPAAYPTLCKGRFMVEGRRDHALEEPTSLNALSLLPRLAAIGVRAIKIEGRQR
                     SPAYVAQVVATLRAALDAAQAAPARYQPHPDWQHTLARHAEGSQTTQGAFDRPWK"
     gene            1213..>2072
                     /locus_tag="PKN55_12635"
     CDS             1213..>2072
                     /locus_tag="PKN55_12635"
                     /inference="COORDINATES: protein motif:HMM:NF011991.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HNX72137.1"
                     /translation="MEMTPPFLITLGPLLYYWPRHTVQAFYREVAASAVDVVYLGETV
                     CLRRHVMRPADWLDIAAELSAAGKQVVLSTPTLVESGAAIQLTHKLAAQDDWLVEAGD
                     LGAIRARGGRPFVAGPHLNAYHGDTLRWLASLGACRFVAPLELNRHELAALLAEAPPG
                     LPCEVMVWGRMPLAFSARCFTARHFNLSKDDCAFRCIEHPDGLAMRTQEDGEFLTLNG
                     VQTQSGAALDLLDQAQALAQLGVAALRVSPQSQGTLPAIAALAAARAGQASPSVTPPA
                     GIGRCNGYWWG"
     assembly_gap    2073..2172
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2272..2682
                     /locus_tag="PKN55_12640"
     CDS             2272..2682
                     /locus_tag="PKN55_12640"
                     /inference="COORDINATES: protein motif:HMM:NF014132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HNX72138.1"
                     /translation="MSVATFGAVVPPALLSPPLCAVLELARLGRWLQPPAELEGRSVA
                     LTLTDLGLRHGLCCRDGRFRPHGVADAELSLSASLADVLLLLAGQVDADTLFFQRRLA
                     IAGDTELGLVVKNWLDAQERPAWLTRLAARWSPM"
     gene            2679..3116
                     /locus_tag="PKN55_12645"
     CDS             2679..3116
                     /locus_tag="PKN55_12645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003748129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc-binding protein"
                     /protein_id="HNX72139.1"
                     /translation="MNAPAAPADKPLVYACSGCSNVAQLANQLAVALDRSGAAQMSCI
                     SGVGGGVATLVKLAQSGRPILALDGCPLACCQACLAQAGVQADQHVLLSELAIRKRHG
                     RDCTVDEQQQAAHAVHTALAKLTGGDLPAVDAGLDGHQANTKC"
     gene            3189..>3501
                     /locus_tag="PKN55_12650"
     CDS             3189..>3501
                     /locus_tag="PKN55_12650"
                     /inference="COORDINATES: protein motif:HMM:NF015442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP cone domain-containing protein"
                     /protein_id="HNX72140.1"
                     /translation="MQSVRKRDGSLAVFNRRNIVRAIAAAGRASGEFDLPLAEALSDQ
                     VLAQLAGERCPDVETIQDRVEETLAQAGHWRTARAYVVYREQHARLRDAARNWVDVGQ
                     CM"
BASE COUNT          519 a         1172 c         1148 g          562 t
ORIGIN      
        1 acgtcgtgat tcagttccac tcataccggg ctgccagctg cgcgatgttc cgtatagcgg
       61 cagcccggcc aacacgccgc caagccagcc ccccaacccg cttgatcccc cctactccca
      121 aagcacacct ccccctccgc caaccggcca gccttgaccc aggataaggc caccgcgtca
      181 cccccgtctt acagtccccg cttgcttcac cacaaggaat gcaaccatgg agctgatcgc
      241 gcccgccggc aatctggcgg cgttaaaggc ggcggtgctg gcgggggcgg atgcggtgta
      301 tctgggcctg aaaaacgcca ccaacgcccg caattttgcc gggcttaact tcaccgaagc
      361 cgacattgcc cagggcgtgg cgtttgcccg ccagcatggc cgccaggtgc tgttcgccat
      421 caataccttt gcccagcctg gccgcgcgca ggaatggcgg gcggcggtgg acgccgcctg
      481 tcggctgggg gcggatgcgc tgattctggc cgaccccggc gtgctggcct acgcccgcca
      541 gcgctacccg cagcagcgtc tgcatttgtc ggtgcagggc tcggccaccc acgccgacgc
      601 catcgagctg atgcgcgagc agtttggcat tcagcgcgtg gtgctgccgc gcgtgctcac
      661 cgtggccgac atcgcccgcc tgattgaaaa aaccacggta gaggtcgagg tgttcggctt
      721 tggcagcctg tgcgtgatgg ccgaggggcg atgtttgctg tccagctttg ccaccggcga
      781 ttcgcccaac aatcgcggcg tgtgctcgcc agcgcatgcg gtgcgctggc agccgcgcga
      841 cgggcagatg gaggcgcgct tgaatggcct gttgatcgac cgctacgccc cggacgaacc
      901 ggccgcctac ccgacgctgt gcaagggccg ctttatggtg gaaggccggc gcgaccatgc
      961 gctggaagag cccaccagcc tgaatgcgct cagcctgctg ccgcgtctgg cggcgattgg
     1021 cgtgcgcgcc atcaagatcg agggccgcca gcgcagcccg gcctatgtgg cgcaagtggt
     1081 ggccaccctg cgcgccgcgc tggacgccgc ccaggccgcg ccagcgcgct atcaacccca
     1141 ccccgactgg caacacacgc tggcccgcca cgccgagggc agccagacca cgcagggagc
     1201 gtttgaccga ccatggaaat gaccccaccg tttctcatca ccctgggccc gctgctgtac
     1261 tactggcccc ggcacaccgt gcaggcgttt taccgcgagg tggccgccag cgcggtggac
     1321 gtggtgtatc tgggcgaaac cgtgtgcctg cgccgccatg tgatgcgccc ggccgactgg
     1381 ctggacatcg ccgccgagct gagcgccgcc ggcaagcagg tggtgctgtc cacgccaacg
     1441 ctggtggaat ctggcgcggc gattcagctc acccacaagc tggccgctca ggacgactgg
     1501 ctggtggaag ccggcgacct gggcgccatc cgcgcccgtg gcggccggcc gtttgtggcc
     1561 ggcccgcacc tgaacgccta ccacggcgac accctgcgct ggctggccag cctgggcgcc
     1621 tgccgctttg tcgccccgct ggagctcaat cgccacgaac tggccgccct gctggcggaa
     1681 gccccgcccg gcctgccctg cgaggtgatg gtgtgggggc ggatgccgct ggcgttttcg
     1741 gcgcgctgct ttaccgcccg gcactttaac ctgagcaagg acgactgcgc ctttcgctgc
     1801 atcgaacacc ccgatggcct ggccatgcgc acccaggaag acggcgagtt tctcaccctg
     1861 aacggcgtgc aaacccaatc cggcgcggcg ctggatttac tcgatcaggc ccaggcgctg
     1921 gcgcagctgg gcgtggcggc gctgcgggtc agcccgcaat cgcaaggcac gctgccagcc
     1981 atcgccgccc tggccgccgc ccgcgccggc caagccagcc ccagcgtgac gccgccggct
     2041 ggcattggcc gctgcaatgg ctattggtgg ggnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2161 nnnnnnnnnn nncgcgcgcc accccgtcat gtccaagccg ctgtgctgcc agcggcatgc
     2221 cgcaaagtct tcaccgccgt cccatcatat tcatgtcaag gagttcaccc gatgtctgtc
     2281 gccactttcg gcgctgttgt gccgccggcc ttgttgtctc ctcctttgtg cgccgtgctg
     2341 gagctggcgc gcttggggcg ttggctgcaa ccgccggccg agttggaggg gcgcagcgtg
     2401 gcgctgacgc tgaccgacct gggcctgcgc catggcctgt gttgccggga tgggcgcttt
     2461 cggccgcatg gcgtggccga cgccgagctg agtttgtcgg ccagcctggc ggatgtgctg
     2521 cttctgctgg ccggccaggt ggacgctgac acgctgtttt ttcagcgccg gctggcgatt
     2581 gccggcgaca ccgagctggg cctggtggtg aaaaactggc tggatgcgca agagcgcccg
     2641 gcctggctga cccggctggc ggcgcgctgg agcccgatgt gaacgcgccg gccgccccag
     2701 cggacaagcc cttggtgtac gcctgttccg gctgttcgaa tgtcgcccag ttggccaatc
     2761 aattggcggt agcactggac cgcagcgggg cggcgcagat gtcgtgcatc agcggggtgg
     2821 gcggcggggt ggcgacgctg gtcaagctgg cgcaaagcgg gcggccgatt ctggccttgg
     2881 acggctgccc gctggcctgc tgtcaggcct gcctggcgca ggcgggggtg caggccgatc
     2941 agcatgtgct gctcagcgaa ttggccatcc gcaaacgtca tggccgcgat tgcacggttg
     3001 acgagcagca gcaggccgcg cacgccgtgc acacggcgct ggcgaagctg accggcggcg
     3061 atttgcccgc ggtggacgcg gggcttgatg gtcatcaagc aaacacaaaa tgctgagttt
     3121 acccaatcaa taaacacaat atgtagtgtt atgatccctc ccatcatcac tctctggggg
     3181 gccgcgccat gcaatcggtg cgcaaacggg atggcagtct tgccgtgttc aaccgccgca
     3241 atatcgtgcg cgccatcgcc gccgccggcc gcgccagcgg cgaatttgac ctgccgctgg
     3301 ccgaggcgct gagcgaccag gtgctggccc aactggccgg ggaacgctgc ccggatgtgg
     3361 aaaccattca ggatcgggtg gaagaaaccc tggcgcaggc cggccactgg cgcaccgcgc
     3421 gggcctatgt ggtgtaccgc gaacaacacg cccgcctgcg cgacgccgcg cgcaactggg
     3481 tggacgtggg ccagtgcatg g
//
LOCUS       DAOPYX010000311         3484 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_36258_length_3484_cov_7.340624, whole genome shotgun sequence.
ACCESSION   DAOPYX010000311 DAOPYX010000000
VERSION     DAOPYX010000311.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 3484)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3484)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3484
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36258_length_3484_cov_7.340624"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(119..319)
                     /locus_tag="PKN55_12655"
     CDS             complement(119..319)
                     /locus_tag="PKN55_12655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX72141.1"
                     /translation="MTDFVGITVFQHTPQCAENPVLFNNPGNYLNPPAKPGAARLTGG
                     SPGKAGQLRQRGIVRCAVIQFA"
     gene            860..3244
                     /gene="amt"
                     /locus_tag="PKN55_12660"
     CDS             860..3244
                     /gene="amt"
                     /locus_tag="PKN55_12660"
                     /inference="COORDINATES: protein motif:HMM:TIGR00836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HNX72142.1"
                     /translation="MFAALRSPAVLTAILATDLAIAPAWAVELHHPPTTQENLNFIWI
                     ILCAALVFFMQAGFSALESGLVRAKNSINVSLKNFSDLIFSMITFFVLGFAFMFGTSG
                     NGWIGLEGFFLEGKSQPYDYAYFIFQAVFAGTAATIVSGAVAERMRFEAYLISTVIIS
                     ALIYPLYGHWAWNSEGWLFKLNFVDFAGSAVVHSIGGWVGLAGALVLGPRLGKFNADG
                     SANDIPASSIPSAVLGAFILWFGWFGFNGGSTLIGDGSIAKIVVNTSISGSVASITAF
                     AISKTLTGKFRAEHILNGALGGLVGVTAGCAVLEPMGALYIGLGAGVVVYFAEVLLLK
                     LKIDDPVGAVPVHGFCGCYGTLALAVFAPADALPMKDHLAQLGVQAIGSATAFAWAFC
                     VGLLLFSLLKWMRILRVPPEYESRGLNEAEHGAKQTMLDTYDTISYMVKSGDFSRKIT
                     PEIGTEAGDIAKVFNLLVDELNDITHVAHNISQGALGKDAMPKSERDMLGQAMASMVL
                     RLRQFAGNLTDITAQLDGSSGELDAASRQLADYNHALLDGVHHVEQLMGEARAASEVM
                     SARSSEGSQSLDQITASMGEISHIMGVFKGNVDSLSLSVADIEQITGMISSIAEQTNL
                     LALNAAIEAARAGEHGRSFAVVADEVRALAGKTQQATADIQHKVRALKTHSAHTVEVT
                     HAGMAAIQQGVTQAAATAGIFHSIFQSADALQDKVTRAAELAMHQARESELSRHAITL
                     TKTIADAMKAQVDNLREMAGFFRLDAQTEGPQTLPDRDCPPDRRASGMVVAGVV"
BASE COUNT          637 a         1028 c         1076 g          743 t
ORIGIN      
        1 gctgcacgcg ctggccagcg atatgcacgg cgcggtgttg cagttccggc tgtaaacggc
       61 ggtcagcaca gccccgagca agacgggctg agcgatgcgg cgtgagcctg cggcttgctc
      121 aagcaaactg aataacagcg cagcgcacaa taccgcgctg gcgcaattgc ccggccttgc
      181 caggcgaccc gccagtcagg cgcgctgcgc cgggttttgc cggcggattt aagtagtttc
      241 ctgggttatt gaataaaacc ggattctctg cgcattgagg ggtgtgctga aaaacggtaa
      301 ttcctacaaa gtcggtcatt cagggttttg agtttgctga acttcgcttt tggtcaatac
      361 ggattttctg aatcactcag cgtggccttg gcgcggcctc ctgagggcgt gacgtgcgct
      421 gcgctaaaac ctgacgcaag ctgactattc cgccaaaagc aaaacatacg gatgtttaat
      481 ttttgaccgg cgctttactc gcattcatcc gcttaatttt ccccagccag aaatacatgg
      541 ccctgcactt gcttgaactc ccgctgcctc acagcgcgtc tttgccaaac atcacgccat
      601 ttcagctaag cgtggctaac aaaaccctcc tgacgttgtg acacgacctt gccgtacgag
      661 ccgtgctgtc tgcggtcgtg cgcctggcca ggatcgctgc gctgagtttt gttcgccacg
      721 ctcattgcct gtcgccaccc cctggtgtgt ggcgacaggc tggatttggc catgttggct
      781 ttcttgcatt gtgtggcaat ccacacattc gctggcgcgc gcaaatgggc cgcgccgcat
      841 gatacgagga ggacaaccca tgtttgccgc acttcgctca cccgccgtct tgacggcgat
      901 tcttgccact gacctggcca tcgcgcccgc ctgggcggtg gaattgcatc atccccccac
      961 cacccaagaa aacctcaact ttatctggat catcctgtgc gcggcgctgg tgtttttcat
     1021 gcaagccggg ttttccgcac tggaatccgg cttggtgcgc gccaagaatt cgatcaatgt
     1081 gtcgctgaag aatttcagcg acctgatttt ttcgatgatc actttttttg tgctgggttt
     1141 tgcctttatg tttggaacca gcggcaatgg ctggattggg ctggaagggt tttttctgga
     1201 aggaaaatcc cagccctacg actacgcgta ttttattttt caggcggtgt tcgctggcac
     1261 ggccgccacc attgtgtcgg gcgcggtggc cgagcgcatg cgctttgagg cgtatctgat
     1321 ttctacggtc atcatctcgg cgctgatcta ccccttgtac ggccattggg cgtggaacag
     1381 cgagggttgg ctgttcaagc tgaattttgt cgattttgcc ggctcggccg tggtgcactc
     1441 gattggcggc tgggtggggc tggccggcgc gctggtgctg gggccgcgcc tgggcaaatt
     1501 caacgctgat ggcagcgcca atgatattcc ggcctcgtcg attcccagcg cggttctggg
     1561 ggcttttatt ttgtggtttg gctggtttgg ctttaatggc ggcagcacgc tgattggcga
     1621 tggcagcatt gccaagattg tggtcaacac ctcgatttcc ggttcggtgg cgtcgatcac
     1681 cgcctttgcc atttccaaaa ccctgaccgg aaaattccgc gccgagcata ttctgaatgg
     1741 cgcgctgggc ggcctggttg gcgtcaccgc cggttgtgcg gtgctggagc ccatgggggc
     1801 gctgtatatt ggcctgggcg ctggcgtggt ggtgtatttt gccgaagtat tgctgctcaa
     1861 gctgaaaatt gacgacccgg ttggcgccgt gccggtgcat ggcttttgcg gctgttatgg
     1921 cacgctggcg ctggcggtgt ttgccccagc cgacgccctg ccgatgaaag accatttggc
     1981 gcaattgggc gtgcaggcga ttggctccgc cacggcgttt gcctgggcgt tttgcgtggg
     2041 cttgctgctg ttttcgctgc tgaaatggat gcgcattttg cgcgtgccgc cagaatatga
     2101 atcacgcggg ctgaacgagg ccgaacacgg cgccaagcaa accatgctcg acacctacga
     2161 caccatcagc tatatggtga aaagcggtga cttcagccgc aaaatcaccc cggaaatcgg
     2221 caccgaagcc ggcgatatcg ccaaggtgtt taatctgctg gtggatgaac tcaatgacat
     2281 cacccatgtt gcgcacaata tttcacaagg cgcgctgggc aaggacgcca tgcccaagag
     2341 cgagcgcgat atgctcggcc aggccatggc ctcgatggtg ctgcgcctgc gccagtttgc
     2401 cggcaatctg accgacatca ccgcccagct ggatggctcg tccggcgagc tggacgccgc
     2461 ctcgcgccag ctggcggatt acaatcacgc cttgctggac ggcgtgcacc atgtagaaca
     2521 gctgatgggc gaggcgcgcg ccgcctcgga ggtgatgagc gcgcgctcca gcgagggcag
     2581 ccagtcgctg gaccagatca ccgccagcat gggcgaaatt tcgcacatca tgggggtgtt
     2641 caaaggcaat gtggattcgc tcagcctcag cgtggccgat atcgagcaga tcaccggcat
     2701 gatcagcagc attgccgagc agaccaatct tttggcgctg aacgccgcca tcgaagccgc
     2761 ccgcgccggc gaacacggcc gcagctttgc cgtggtggcc gacgaagtgc gcgcgctggc
     2821 cggcaaaacc cagcaggcca ccgccgacat tcagcacaag gtgcgcgcgc tgaaaaccca
     2881 ttcggcgcac accgttgaag tcacccacgc cggcatggcc gccatccagc aaggggtgac
     2941 gcaggccgcc gccaccgccg ggatttttca cagcattttc cagagcgccg acgcgctgca
     3001 agacaaagtc acccgcgccg ccgagctggc catgcatcag gcgcgagaaa gcgagctgtc
     3061 ccgccacgcc atcaccctga ccaaaaccat tgccgacgcc atgaaggccc aggtggacaa
     3121 cttgcgcgag atggcgggtt tcttccggct ggatgcgcag accgagggcc cacagaccct
     3181 gccggaccgt gactgcccgc ccgacaggcg agcttctggc atggtggtgg ctggagtggt
     3241 gtagcaccgc tgacaacact cagcgaagcg gccctggcga ctaagcgagt gaccgcagat
     3301 cgcaccacca gtacggccag gtgacacacc accagggcgc gctgccggta ctgtctgcgg
     3361 tggcgtgcgt tgtgcgcgcg gcgtggtgga gagcggcgct tggcgggtgg ccgtggcccc
     3421 ctgcggacgg cgttctgggc gataatccgg ccatgaccac ttggctagac agcgtggcgg
     3481 ggat
//
LOCUS       DAOPYX010000312         3472 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_36481_length_3472_cov_3.111794, whole genome shotgun sequence.
ACCESSION   DAOPYX010000312 DAOPYX010000000
VERSION     DAOPYX010000312.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 3472)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3472)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3472
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36481_length_3472_cov_3.111794"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1055
                     /gene="feoB"
                     /locus_tag="PKN55_12665"
     CDS             <1..1055
                     /gene="feoB"
                     /locus_tag="PKN55_12665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011137100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HNX72143.1"
                     /translation="EGLGEGLDWVQAQWWQPALASAPAWLQSLLLEGVWQGVSTVLTF
                     VPILFVFFVLMAVVEDSGYLARAAFLMDAWMTRLGLDGRSFVMHLMGFGCNVPAILGT
                     RIMRERRLRWLTMLVIPFSVCSARLQVFLFFVSTLFSAQAAPWVLLSLYLASFLVAML
                     TAWLFKRALPHREALVMEMPPYRLPVLAYLFTRAGGEVKAFLQLASTFILAGVVLIWA
                     LTHLPAAGGPTLAAQLGEWLAPVLDPLGIRHELAVALMVGVVAKEILLGGMAVIYGVP
                     EAGLAQVISQQLDWVAAYSFMLFTLIYTPCLSTIAAIRKESRSNGFAALSVGYSLALA
                     WCVAALFYQGARWWLG"
     gene            1122..3458
                     /locus_tag="PKN55_12670"
     CDS             1122..3458
                     /locus_tag="PKN55_12670"
                     /inference="COORDINATES: protein motif:HMM:NF025214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNX72144.1"
                     /translation="MSAQSVFSMSDASEPLSIDTEIDLKAAFDSAIVHHQAGRLSQAE
                     LLYRMVLRTLPLHPDANHNLGVIAVQLGQPEHALPLLNTALTQDLTQGRFWISYIDAL
                     LRAGHLDEADQWLAQGRKSGLAGPQVDELAQRLAVAREPKPLAKAGKNRKAKHAQPPL
                     AVQQKLVALFNQGQTEACEQLARELTETYPRHPLAWKTLGAVVTQLGRTAEGVQIMRT
                     AAELAPQDVELLSNLGVTLVMMGQLEEAEHCYRRALKLQPHSSSLHAFLADTLFLLLR
                     YPQAEQHARKAIELNPNNEIAYHNLGSVLRASSKISEAESVLRHALSLAWNYPAAHAN
                     LANVLKDQGRLVEAEKHLRLALDMQPNNRMIRSNLLFATIYSHTVTPEQLYADHCVYG
                     RMISAMAGPSMHLSRDLSPQPRRIGFVSADLFHHAVSYFVEPVWDGLRERGCTVLAYY
                     NGKHYDGATARLHRKCDVWRDVVGMPDEQLAQQIVDDEVDVLIDLSGHTGGNRLPVFA
                     REPAPLQVTWLGHPATTGLPGMHWRITDVYADPPGMTEHLNTERLYRMPEVFCCYHPQ
                     NEPALAEQLPVEHTGRFTFGCFNNFAKVTPPVIACWGGILRQLPQSRLLLEVHGAEDV
                     AFAADIRQRFVDAGASPAQIDILPRQSGQQYVLYREVDLALDPFPCSGGTTSCDSLWM
                     GTPFVTLAGRSFVSRMGVTFLKNVGLDELIADNEADYQAKALALAQDLPRLRALREGL
                     RERVRQSPLMDAPRFAGHFAQALADMWAEKIAAQTKSG"
BASE COUNT          587 a          930 c         1125 g          830 t
ORIGIN      
        1 aagaagggct gggcgaaggg ctggactggg tgcaggcgca gtggtggcaa ccggcgctgg
       61 ccagcgcgcc cgcgtggctg caaagcttgc tgctggaagg cgtgtggcag ggggtgagca
      121 cggtgctgac ctttgtgccc atcctgtttg tgttttttgt gctgatggcc gtggtggaag
      181 acagcggtta tctggcgcgc gcggcttttt tgatggacgc ctggatgacc cggctggggc
      241 tggatggccg cagctttgtc atgcatctga tgggctttgg ctgcaatgtg ccggccattc
      301 tgggcacgcg catcatgcgt gagcgtcggc tgcgttggct gaccatgctg gtgattccgt
      361 tttcggtgtg ctcggcgcgg ctgcaagtgt ttttgttttt tgtcagcacg ctgttttctg
      421 cccaggcggc gccgtgggtg ttgctgagcc tgtatctggc cagttttttg gtggcgatgc
      481 tgaccgcctg gctgttcaag cgcgccttgc cgcatcgcga ggcgctggtc atggagatgc
      541 cgccctaccg tttgccggtg ctggcctatc tgttcacccg cgctggcggt gaggtgaagg
      601 cgtttctgca attggccagc acttttattc tggctggcgt ggtgttgatc tgggcgctga
      661 cccacctgcc ggcggccggc ggcccgaccc tggcggcgca attgggtgaa tggctggccc
      721 ccgtgctgga tccgctgggc atccgtcacg agctggcggt ggcgctgatg gtgggcgtgg
      781 tggccaaaga aatcctgctg ggtggtatgg cagtgattta tggcgtgccg gaggccggcc
      841 tggcgcaggt gatcagccag cagctggatt gggtggcggc gtacagcttt atgctgttca
      901 cgctgattta cacgccttgc ttgtctacca ttgccgccat tcgtaaagaa tcgcgcagta
      961 atggctttgc cgcgctgtcg gtgggttatt cgctggcgct ggcctggtgt gtggcggcgt
     1021 tgttttatca gggcgcgcgc tggtggctgg gctgaagccg gtgtgtatcg ccatgaggtt
     1081 ctccacggtg gtggcgtcat gcgttacact gccggcattg catgtctgcc caatcggttt
     1141 tttctatgtc cgacgcttct gagccgctct ccattgatac agaaatcgat ctgaaagcgg
     1201 cgtttgactc cgccattgtt caccatcagg ctggccgttt aagccaggcg gagttgctgt
     1261 atcgcatggt gctgcgcacc ctgcccttgc atcctgacgc caatcacaat ctgggcgtga
     1321 tcgccgtaca gttggggcag ccagagcatg ccttgccctt attgaatacg gcgcttacgc
     1381 aggatctgac gcaagggcga ttctggatca gctacattga tgcattgttg cgcgcgggcc
     1441 atcttgatga ggcggatcag tggctggcgc aaggccgcaa aagcggcctg gccgggccgc
     1501 aagtggatga gctggcgcaa cggctggctg tggcgcgtga gcctaaaccc ttggccaaag
     1561 ctggaaaaaa tcgcaaagcc aagcacgccc agccgccctt ggccgtccag caaaagctgg
     1621 tggccttatt caatcagggg cagaccgagg cgtgcgagca actggcgcgc gagttgaccg
     1681 aaacttaccc gcgccaccca cttgcctgga agaccttggg cgccgtggtg acccagcttg
     1741 gccgcacggc tgaaggggtg cagatcatgc gcacggctgc agaacttgct ccccaagatg
     1801 ttgagttgct cagcaactta ggggtgacgc tagtgatgat gggtcaattg gaagaggctg
     1861 agcattgtta ccggcgcgcc ttgaagctcc agccgcactc ctccagcttg catgcttttc
     1921 tggccgacac cttgtttttg ttgctgcgct accctcaggc tgaacagcat gcgcgcaagg
     1981 cgattgagct gaacccgaat aatgaaattg cttaccacaa tctgggttcg gtgttgcggg
     2041 cttcgagcaa gatcagcgag gcggagtctg ttttgcgtca tgcgctctcg ctggcctgga
     2101 attatcctgc cgctcacgcc aatctggcta atgtgctgaa ggatcagggg cggctggtgg
     2161 aggcggaaaa gcatttgcgt cttgccctgg atatgcagcc caacaatcgc atgattcgaa
     2221 gcaatctttt atttgccacg atttatagtc acactgtcac acctgagcag ctgtatgctg
     2281 atcattgtgt ttacggtcgc atgatttccg ccatggctgg cccgtctatg cacttatctc
     2341 gcgatttgtc gccacaaccg cgccgaatcg gttttgtttc tgctgacttg tttcatcatg
     2401 ctgtcagtta tttcgtggag ccggtctggg atggcctgcg ggagcgaggc tgcacggtgc
     2461 tggcgtacta caacggcaag cattacgatg gcgccacggc gcgcttgcac cgcaagtgtg
     2521 atgtttggcg tgatgtggtc ggcatgccgg atgagcagct ggcgcaacaa attgtcgatg
     2581 acgaagtcga tgtcctgatt gatttgtctg gccatacagg cggcaatcgg ttgccggtgt
     2641 ttgctcgtga acctgcgcca ttacaagtga cttggttggg gcatcctgcc accacgggtt
     2701 tgccgggcat gcattggcgg ataacggatg tgtatgcgga tcctcctggg atgactgaac
     2761 acctgaatac cgagcgcttg tatcgcatgc ctgaagtatt ctgttgctat catccacaaa
     2821 acgagcctgc gctggcggag caattgccgg ttgagcacac ggggcgcttc acttttggct
     2881 gcttcaacaa ttttgctaaa gtgacgccgc cggtgattgc ctgctggggc ggcattttgc
     2941 gccagttgcc gcaatcacgc ttgctgctgg aagtgcacgg cgcagaagat gtcgcctttg
     3001 ccgccgatat tcgtcaacgt tttgtcgatg cgggcgcatc gccagcacag atcgacattc
     3061 tgccgcgtca gtctggccag cagtatgtgc tctatcgcga ggtggatctg gcgctggatc
     3121 ccttcccatg ctctggcggc accaccagtt gcgacagcct gtggatgggc acgccgtttg
     3181 tcaccctggc ggggcgcagc tttgtgtcgc gcatgggcgt cacgttcctg aaaaatgtcg
     3241 ggctggacga attgatcgct gacaacgaag cggactatca ggccaaggcg ctggcgctgg
     3301 cgcaggactt gccgcgtttg cgcgcgctgc gcgagggatt gcgcgagcgt gtgcggcaga
     3361 gtccgctgat ggacgcccca agatttgctg gccattttgc ccaagcgttg gcggacatgt
     3421 gggcagaaaa aatcgccgcg cagactaaga gcggttaacc aaaccctcct gg
//
LOCUS       DAOPYX010000313         3471 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_36489_length_3471_cov_8.623829, whole genome shotgun sequence.
ACCESSION   DAOPYX010000313 DAOPYX010000000
VERSION     DAOPYX010000313.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 3471)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3471)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3471
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36489_length_3471_cov_8.623829"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            111..287
                     /locus_tag="PKN55_12675"
     CDS             111..287
                     /locus_tag="PKN55_12675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX72145.1"
                     /translation="MRLYATQRSHADSIRHDLTEACQRLDQHQQRLQDLLDTIGPIDI
                     GRGDDSSEESGECA"
     gene            278..1744
                     /locus_tag="PKN55_12680"
     CDS             278..1744
                     /locus_tag="PKN55_12680"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNX72146.1"
                     /translation="MRLSIRSKLYVFSAVAMLALAAMGALGLAAGYLADVQLHRLRHD
                     HGLALARLSQGEATMRASPPAWPMLTALASNPPELFAEPTLQAPWQAWQHAVASQNPA
                     AMQQHWATLAHQTRALIDREQSEQDQLRHQLFQLILWGGAALSIGLLALARWIIRTIV
                     RPLRALEATASQTASDLDLTRRCQLRGHDDEVAHSIQAFNALLDAIGDSFRLAETASS
                     QVDGSAEEVAGASQQIARAAEAQSESTLSMAFAIERFQIGVRQSREEAHFALESASLA
                     ERLASESDQVIIKAALDIQDMVAAIQRSAQDVSQLAERSEEINRVVQLIANVADQTNL
                     LALNAAIEAARAGEQGRGFAVVADQVRMLAETTRSATREIGGSIQWVQEQTRHAADNL
                     LQGEKLVSFGVELMHGLARPLEEMRDSARTAHDKLDHLMETLAQQQEASDTIGKDIEA
                     LTVISEQNSAAASQSANAALTLHEAVRTLSSQLARFGF"
     gene            2097..>3471
                     /locus_tag="PKN55_12685"
     CDS             2097..>3471
                     /locus_tag="PKN55_12685"
                     /inference="COORDINATES: protein motif:HMM:NF028509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNX72147.1"
                     /translation="MKISHRLALIILCAATGFVILCAYSLYVVRDSMISERKAATEIH
                     VRMAGNLIAQFQAAEKAGKLSREEAQRQAAQAIRGMRYQGDYMYLRSFSGLSIVHPDK
                     RKEGKTDGGGTLSDGRTLMQGYRDVLANSDFGYVSLYTKRVNGNVDVPKINGVLKIAD
                     WDWIVGTGVFLDDIDAIFWKRVWQFLGIGVVVLSCVIGLAVWMSRSIYRVLGGEPAYA
                     AQVAQAIAAGTLNQRIDGQANTDSLLGAMARMQEQLRGMVSLIQHNASQLNHSAHGIN
                     QQMVDMTDMANQASDAATSTTAAIQQLSNSVLHISDNAQRTQTQSARAATLAADGGAL
                     VNDAASHIQHVSEQLEQASSQIAALDQRAAEIGGIAGVIKDIADQTNLLALNAAIEAA
                     RAGEQGRGFAVVADEVRKLAERTTEATTQISNMIGAVQRDTRDVVGSMGAAAPQVESS
                     VGKAQSAA"
BASE COUNT          725 a         1053 c         1032 g          661 t
ORIGIN      
        1 tatttgcagg agaatgccgg aaactgcccc ggagagcgtc acactttaca agaacttaac
       61 atttcaaaag catacttcgc ctggttccct tacttgtctc aggtgaagcc atgcgcctct
      121 atgccaccca gcgttcgcac gccgattcga tccgtcatga tttgaccgaa gcctgccaac
      181 ggcttgacca gcaccaacag cgcctgcaag acctgctgga cacgattggc cctattgaca
      241 tcggccgtgg cgacgacagt agcgaggaga gtggtgaatg cgcctgagca tccgcagcaa
      301 actctatgtg ttcagtgctg tggccatgct ggcgctggcg gccatgggtg cgctgggcct
      361 ggcggccggt tatctggccg atgtgcaatt gcaccgcttg cgccacgatc acggcctggc
      421 actggcccga ctcagccaag gtgaagccac catgcgcgcc tcgccaccgg cctggccgat
      481 gttgaccgcc ctggccagca acccgccgga actgtttgcc gagccgacgc tacaggcacc
      541 ctggcaggcc tggcaacatg ccgttgccag ccagaatccg gcggcgatgc aacagcactg
      601 ggccacactg gcacaccaga cccgcgcgtt gatcgaccgg gagcaaagcg aacaggatca
      661 acttcgtcat cagctgtttc agctgatttt gtggggcggt gcagcgctca gcatcggcct
      721 gctggcgctg gcgcgctgga tcatccgcac cattgtccgc cccttgcgcg cgctggaagc
      781 caccgccagc caaaccgcca gtgaccttga cctgacccgc cgctgccaac tgcgcggcca
      841 tgatgatgaa gtggctcaca gcattcaggc tttcaacgcc ctgctggatg ccatcggcga
      901 cagcttccgg ctggcagaga cagccagcag ccaggtggat ggcagcgccg aagaagtggc
      961 cggtgcctca cagcagattg cccgcgctgc cgaagcacaa agtgaatcca ccctgagcat
     1021 ggcttttgcc atcgagcgtt ttcagattgg cgtacggcaa agccgcgaag aagcccactt
     1081 tgccctggaa tccgccagcc tggccgagcg gctggccagc gagagcgatc aagtgatcat
     1141 caaggcagcg ctggacattc aggacatggt tgccgccatc cagcgctcgg cgcaggatgt
     1201 gtcgcagttg gccgagcgct cggaagaaat caaccgcgtg gtgcaattaa ttgccaatgt
     1261 ggccgaccag accaatctct tggcgctgaa cgcggcaatt gaggccgccc gcgccggcga
     1321 acagggccgc ggctttgccg tggtggccga tcaggtgcgc atgctggcag aaaccacgcg
     1381 cagcgccacc cgcgagattg gcggctcgat tcaatgggtg caagagcaaa cccgccatgc
     1441 tgccgacaat ctgctgcaag gtgaaaaact ggtgagcttt ggcgtggagc tgatgcacgg
     1501 cctggcccgc ccactggaag aaatgcgcga cagcgcccgc actgcccacg acaagctgga
     1561 tcatctgatg gaaaccctgg cccagcaaca ggaagccagc gataccatcg gcaaggacat
     1621 cgaagccttg actgtcatca gcgaacaaaa cagcgccgcc gccagccaga gcgccaacgc
     1681 agcgctgact ctgcacgaag cggtgcgcac actcagcagt caattggcgc gtttcgggtt
     1741 ttaagcgtca cgcccaaaac ccgctggcgt tgttgtgtga acttgccgta cacccatact
     1801 gtctgtggtc acccacttgg ccagggtcgc ttcgctgagt ttgccaagtc acgctaaaca
     1861 gccactcacc gccagtgacg cgatattgtc cggcttggtc ccaggcaaga atcccgtcat
     1921 gatatcgcgc tgttttttat ctcattttgt tgataatggt ttttcttttg aatttttctt
     1981 gcgccgctgg gcgtgtgtgc ggcagatagt gcttgccttg cttcatgaaa ccgtcttaac
     2041 ttggttttac aattcagtat ttctacggtt tctttctgca agagtaggtg cagcccatga
     2101 agatctccca ccgcctggcc ctcattattt tgtgcgccgc aacaggcttt gtcattctct
     2161 gcgcctactc tttgtatgtg gtgcgcgact cgatgatctc cgagcgcaaa gcagccactg
     2221 aaattcacgt gcgcatggcc ggtaatttga ttgcccaatt ccaggccgcc gaaaaagccg
     2281 gcaagctcag ccgggaagaa gctcagcgcc aggcggcaca agccattcgc ggcatgcgct
     2341 accaaggcga ttacatgtat ctgcgcagct tttctggctt atctattgtg cacccggaca
     2401 agcgcaaaga aggcaagaca gatggtggag gcaccttgtc agacggtcgc accctgatgc
     2461 agggataccg ggatgtgctg gcaaacagcg attttggcta tgtatctttg tatacaaagc
     2521 gtgtgaacgg caatgtggac gtgccaaaaa tcaacggcgt gctgaaaatt gcggattggg
     2581 actggattgt tggcacgggg gtatttctgg acgatatcga tgccatcttc tggaagcggg
     2641 tatggcagtt tcttggcatt ggcgtggtcg tgctgagctg cgtcattggt ctggcggtgt
     2701 ggatgtcacg gtcgatctac cgggtgctgg gcggcgagcc tgcctatgcc gcacaagtgg
     2761 cgcaagcgat tgccgccggc acgctgaatc agcgcattga tggccaggcc aacaccgaca
     2821 gcctgctggg cgccatggca cgtatgcagg aacagctgcg cggcatggtc agcctgatcc
     2881 agcacaatgc cagccagctg aatcactcgg cgcatggcat caaccagcag atggtggaca
     2941 tgaccgatat ggccaatcag gcctcggacg ccgccacctc gaccacggcg gcaattcagc
     3001 aactgtcgaa tagcgtgttg cacatctcgg acaacgccca acgtacgcaa acccaatccg
     3061 cacgcgccgc caccctggct gccgacggcg gggcgctggt caatgatgcg gccagtcaca
     3121 tccagcatgt ttccgaacag ctggagcagg catccagcca gattgccgcg ctggatcagc
     3181 gcgcggccga gattggcggc atcgccgggg tgatcaagga cattgccgac cagaccaatc
     3241 tcctggcgct gaacgcggcc attgaggccg cacgcgccgg cgaacaaggc cgtggctttg
     3301 ccgtggtggc cgacgaggtg cgcaaactgg ccgagcgcac caccgaagcc accacacaaa
     3361 tttccaacat gatcggcgcg gtgcagcgcg acacccgcga cgtggtgggc agcatgggcg
     3421 ccgctgcgcc gcaggtggaa agcagcgtcg gcaaggcgca gtcggcggcg c
//
LOCUS       DAOPYX010000314         3465 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_36578_length_3465_cov_7.256305, whole genome shotgun sequence.
ACCESSION   DAOPYX010000314 DAOPYX010000000
VERSION     DAOPYX010000314.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 3465)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3465)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3465
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36578_length_3465_cov_7.256305"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(50..802)
                     /locus_tag="PKN55_12690"
     CDS             complement(50..802)
                     /locus_tag="PKN55_12690"
                     /inference="COORDINATES: protein motif:HMM:NF016296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HNX72148.1"
                     /translation="MPTTPRYLIVDDEPRLAERLAQRLAVAWPQAECLGLWHDGLSAR
                     EAIASQRPDVAFLDIRMPGMDGLLLAQHQLGQADAPLIVFVTAYQEHALEAFGVAAVD
                     YLCKPVDPLRLAATVQRLQGLLAARTASAPGISANPDGGNGPLRYLRAGQGDTVHLVP
                     VEQVMYLEAADKYVRAVTEHTEHWLRSSLKTLASQLDSDDFWPIHRSLLVNVRYVDAA
                     HRDERGKWQLSLRGRSERLPVAAQYVHLFRQG"
     gene            complement(771..1850)
                     /locus_tag="PKN55_12695"
     CDS             complement(771..1850)
                     /locus_tag="PKN55_12695"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HNX72149.1"
                     /translation="MRPAEAGRPRFFPLRELLLGCILTNIVLALIFTPMSVTTLWQNL
                     VYNHTISGSILLLEELVRRRFWGHSWPPRHWMALLMLVAVPLGGAIGLALGSWLTDTS
                     PMDGPLPLSAAANLWIVLTITLAASGVLLGGLWQRSRLLEARASAQAAQRGQTLAELH
                     ALQAQVEPHFLFNTLAHLDALISQPTRHEARALLSHLTDYLHRTQPTARPAASQLSDE
                     LARIEPWLHILAIRFGARLRWRIHASQQAASCQLPPMLLQPLVENAIRHGIEPAVQGG
                     ELSIYADLYDSALHIRVCDNGVGLNAPNARPGAGSGLANIRARLAALYGHAASLTLTP
                     LAPHGVEARLILPAHYADHTPLSDR"
     gene            complement(1847..2923)
                     /locus_tag="PKN55_12700"
     CDS             complement(1847..2923)
                     /locus_tag="PKN55_12700"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HNX72150.1"
                     /translation="MVKPARAVPDIALLPLRELVLSLLLLNTLIATLLTLILPLSTFW
                     VNMVYSHLIGACICTLTEVGQRLLWTRCGWPTPWRFGLLLLGSTVLGASLGATLAGWV
                     THFSLHEGPIGDLLSNVLAVLGISMLASLTLSAWLWQRGRLAEARLDAETALRGQTLA
                     ELRALQAQVEPHFLFNTLAHLDALLQNDTLDEAQDLLDYFTRYLRGTLSTTRQEASPL
                     ADELARIEPWLRILSIRFGQRLRWRIDASEQAAHCLLPPMLLQPLAENAIRHGIEPAV
                     GGGVLHIDAQVHDGALCVCVRDDGVGLEAAHARPGAGSGLANIRARLAALYGDAASLS
                     LTPLAPHGVEARLSLPAQRPGPRT"
     gene            complement(2917..3324)
                     /locus_tag="PKN55_12705"
     CDS             complement(2917..3324)
                     /locus_tag="PKN55_12705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX72151.1"
                     /translation="MPITPPLSELWRAMPPVIATHALAAGYSLLCGLWLLARRRRGDG
                     LHRGLGVSWMLAMAVTAVGSFWIQSQGHLSWIHLLSVLTLFGLGQNLYALRHGRQQMH
                     YKAVRGMMIGLGVAFVFTLSPDRILGHWLWAQW"
BASE COUNT          606 a         1088 c         1228 g          543 t
ORIGIN      
        1 gggcatgcaa gacgggtatt gtggcacagc cagccgtgat tgtggggcct cagccctggc
       61 ggaacaggtg cacatactgc gccgccactg gcaggcgctc gctgcggccg cgcaggctga
      121 gttgccattt gccgcgttcg tcgcggtggg cggcgtctac gtagcgcaca ttcaccagca
      181 ggctgcggtg aatcggccag aagtcgtcgc tgtccagctg gctggccagg gttttcagcg
      241 agctgcgcag ccagtgttcg gtgtgttcgg tgacggcgcg cacgtatttg tctgcggctt
      301 ccaggtacat cacctgttcc actggcacca gatgcacggt gtcgccctgg ccggcgcgca
      361 gatagcgcag cggcccgttg ccgccgtcgg ggttggcact gatgccaggg gcgctggcgg
      421 tgcgcgcggc cagcaggcct tgcaggcgct gcacggtggc ggccaggcgc agggggtcta
      481 ccggtttgca cagatagtcc accgccgcca cgccaaaggc ttccagcgcg tgttcttgat
      541 aggcggtgac aaacacaatc agcggggcgt cggcctggcc cagttggtgt tgggccagca
      601 gcaggccgtc catgccgggc atgcggatat ccagaaaagc cacgtccggg cgctggctgg
      661 cgatggcttc gcgggcggaa agaccatcgt gccacagccc caggcactcg gcctgcggcc
      721 aggccacggc caggcgttgc gccaggcgtt cggccaggcg gggttcgtcg tcaacgatca
      781 gatagcgggg tgtggtcggc atagtgggcg ggcagaatca ggcgggcttc cacgccgtgt
      841 ggcgcaagcg gggtgagggt gaggctggcg gcgtggccat acagtgcggc cagccgggcg
      901 cggatattgg ccaggccgct gccagcgcca gggcgggcgt tgggggcgtt caggcccacg
      961 ccgttgtcgc aaacgcggat atgcagcgcg ctgtcgtata aatcagcata aatactcagc
     1021 tcgccgcctt gcacggcagg ttcgatgccg tggcggatgg cgttttccac cagcggttgc
     1081 agcagcatcg gcggcaactg gcagctggcg gcttgctggc tggcgtggat gcgccagcgc
     1141 aggcgggcgc caaagcggat ggccaggatg tgcagccagg gttcgatgcg cgccagttcg
     1201 tcgctcaact ggctggcggc cgggcgggcg gtgggttgag tgcggtgcag atagtcggtc
     1261 aggtggctga gtagggcgcg tgcctcgtgg cgggtgggct ggctgatcag cgcatccaga
     1321 tgggcgaggg tgttgaacaa aaaatgcggt tctacctggg cttgcagcgc gtgcagctcg
     1381 gccagggttt gcccgcgctg cgccgcctgg gcgctggcgc gcgcttccag caggcggctg
     1441 cgttgccata gcccgcccag cagcacgccg ctggcggcca gggtgatggt gagcacaatc
     1501 cacagattgg cggcggcgct cagcggcaac gggccgtcca tgggcgaggt gtcggtcagc
     1561 cagctgccca gcgccagacc gatggcgccg cccagcggca cggccaccag catcagcagc
     1621 gccatccagt ggcgcggcgg ccagctatgg ccccaaaaac gtcggcgcac cagttcttcc
     1681 agcagcagga tgctgccgct gatggtgtgg ttgtacacca ggttttgcca cagggtggtg
     1741 accgacatcg gggtaaagat cagcgccagg acaatattgg tgaggataca gcccagcagc
     1801 agctctcgca gcggaaaaaa acgcgggcga cccgcttcag ctggcctcat gtgcgtggtc
     1861 ctggccgctg ggccggcagg ctcagacggg cttccacgcc gtgtggcgcc agtggggtga
     1921 ggctcaggct ggcggcgtcg ccatacagcg cggccaagcg ggcgcggata ttggccaggc
     1981 cgctgcctgc gccaggccgg gcgtgcgcgg cttccagccc cacgccgtcg tcgcgcacgc
     2041 acacgcacag cgcaccgtcg tgcacctggg cgtcgatatg cagcacgccg ccgcccacgg
     2101 cgggctcgat gccgtggcga atggcgtttt ctgccagcgg ttgcagcagc atcggcggca
     2161 gcaggcaatg cgcggcctgc tcgctggcgt caatgcgcca gcgcaggcgc tggccaaagc
     2221 ggatggacaa gatccgcagc cagggttcga tgcgcgccag ctcatcggcc agcgggctgg
     2281 cctcctggcg ggtggtggac aaggtgccgc gcaaatagcg ggtaaagtaa tccagcaagt
     2341 cttgcgcctc gtccagggtg tcgttttgca gcagcgcatc cagatgcgcc agggtattga
     2401 acaaaaaatg cggctcgacc tgggcttgca gcgcgcgcag ctcggccagg gtttgcccgc
     2461 gcagggcggt ttcggcgtcc agccgggctt cggccagccg gccgcgctgc cacagccagg
     2521 ctgatagtgt caggctggcc agcatggaga tccccagcac ggccagcaca ttgctgagca
     2581 aatcgcctat cgggccttca tgcagcgaaa aatgcgtcac ccaaccggcc aaggtggcgc
     2641 ccaggctggc gcccagcacg gtgctgccca gcaataacag gccaaagcgc catggcgtgg
     2701 gccagccgca gcgcgtccac agcagacgct ggcccacttc ggtcaacgtg cagatgcagg
     2761 cgccaatcag gtggctgtac accatgttca cccaaaaggt ggacagcggc aaaatcagcg
     2821 tcagcaatgt ggcaatcagg gtattcagca gcaataggct gagcaccagc tcacgcagcg
     2881 gcagcagcgc gatatctggc acggcgcgcg cgggttttac cattgtgccc atagccagtg
     2941 ccccagaatc ctgtctggcg acagggtaaa cacaaacgcc acacccaagc cgatcatcat
     3001 gccgcgcacc gccttgtagt gcatctgctg ccggccatgg cgcagggcat acaggttttg
     3061 ccccaggcca aacagggtca ggaccgacag cagatgaatc cacgacagat ggccctggct
     3121 ttgaatccag aacgaaccca ccgccgtgac cgccatggcc agcatccagc tgacgcccag
     3181 cccgcgatgc aggccatcgc cccgacgacg gcgcgccagc agccacaggc cgcacagcag
     3241 gctgtagcct gcagccaggg cgtgggtggc gatgactggc ggcatggctc gccacagttc
     3301 ggacaagggg ggcgtaatgg gcatcgagcg cttctccagc agcatctatc tgggcggcag
     3361 tgtcgcgcag tgcgggccaa cttgccagcc gcatgcgacc agggcggggt ttggcggcgc
     3421 gaaacgcggc atctgtgcgc cacggattca cgccgtgaat aaagt
//
LOCUS       DAOPYX010000315         3455 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_36737_length_3455_cov_11.225588, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000315 DAOPYX010000000
VERSION     DAOPYX010000315.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 3455)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3455)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3455
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36737_length_3455_cov_11.225588"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..459
                     /gene="leuD"
                     /locus_tag="PKN55_12710"
     CDS             <1..459
                     /gene="leuD"
                     /locus_tag="PKN55_12710"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018747773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HNX72152.1"
                     /translation="RPVNPDFVLNFPRYQGAQVLLARENFGCGSSREHAPWALEDQGL
                     RVVIAPSFADIFFSNCYKNGLLPIVQPAEVVDQLFKECVASEGYRLTVDLAAQTVTTP
                     SGQSFAFDITPHRKHCMLNGLDEIGLTLAHADKIRAFEDARKQSLPWFYV"
     gene            569..1636
                     /gene="leuB"
                     /locus_tag="PKN55_12715"
     CDS             569..1636
                     /gene="leuB"
                     /locus_tag="PKN55_12715"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018747771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HNX72153.1"
                     /translation="MKIAVLPGDGIGPEIIAQALRVLDVLKAEGLPIETETGLIGGCA
                     VDATGVPLPDATLKLALEADAVLLGAVGGPKYDTLPREQRPERGLLKIRKEMNVFANL
                     RPAILYPELANASSLKPEVVAGLDIMIIRELVGDIYFGEPRGIEVRNGEKVGFNTMIY
                     SESEIRRIAHVAFKTAQKRSKKLCSVDKMNVLESTQLWRDVVTEVAAEYPDVELSHML
                     VDNAAMQLVKNPKQFDVMVTGNIFGDILSDEASMLTGSIGMLPSASLDANGKGLYEPI
                     HGSAPDIAGKGLANPLATILSVAMMLRYTFNQDAAADRIEAAVKSVLAQGLRTADIYE
                     DGATRVGTVEMGDAVIKALTA"
     gene            1732..2844
                     /gene="asd"
                     /locus_tag="PKN55_12720"
     CDS             1732..2844
                     /gene="asd"
                     /locus_tag="PKN55_12720"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014086696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HNX72154.1"
                     /translation="MKVGLVGWRGMVGSVLMQRMVEENDFAHIDPVYFTTSNPGGAAP
                     SFGGKTAPALKDAKNLDELKACDVIITCQGGDYTTEVYGPLRAAGWNGYWIDAASTLR
                     MEDEAVIILDPVNMNVIKDSLAKGGKTFVGGNCTVSLMLMALGGLFQNDLVDWATSMT
                     YQAASGAGANNMRELLKGMGAVNAAVAAELADKNSAILDIDRKVAEFIRSDANPTEYF
                     GVPLAGSLIPWIDKDLGNGQSKEEWKGGVETNKILGRSDSPIVIDGLCVRIGAMRCHS
                     QALTIKLKQDLPVAEIEALLAKANDWVKVVPNQREASMKDLTPAAVTGTLTVPVGRIR
                     KLGMGGEYISAFTCGDQLLWGAAEPLRRMLRIVLDK"
     gene            complement(3264..>3455)
                     /locus_tag="PKN55_12725"
     CDS             complement(3264..>3455)
                     /locus_tag="PKN55_12725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX72155.1"
                     /translation="TDSATHRTKSRSQNPLALLCDFAVLLVLSAVTRLARTASLGFMS
                     GSNLVQFTPHGNRVRHAVA"
BASE COUNT          739 a         1108 c         1039 g          569 t
ORIGIN      
        1 cgcccggtga acccggactt tgtgctgaac ttcccgcgct atcagggcgc gcaagtgctg
       61 ctggcgcgtg agaactttgg ctgcggcagc tcgcgcgaac acgcgccgtg ggcgctggaa
      121 gaccaaggcc tgcgcgtggt catcgccccg tcgtttgccg atattttctt tagcaactgc
      181 tacaaaaacg gcctgctgcc gattgtgcag ccggccgaag tggtggatca gctgtttaaa
      241 gaatgcgtgg ccagcgaagg ctaccgcctg accgtggacc tggccgcaca gaccgtcacc
      301 accccgtccg gccagtcgtt tgcctttgac atcaccccgc accgcaagca ctgcatgctc
      361 aacggcctgg acgaaatcgg cctgaccctg gcgcacgccg ataaaatccg cgcgtttgaa
      421 gacgcccgca agcaaagcct gccgtggttc tacgtgtaag ctgagcgggt ggcgcagtgt
      481 gcgcggatgg cccttgacgg tggccaatca tggcatactg cgccatcggc gcactgattc
      541 agaacattgt ttgacaagga aaactcaagt gaaaattgct gttctgccgg gtgacggcat
      601 tggtccggaa attattgcgc aagcgctgcg cgtgctcgac gtgctcaagg ccgagggcct
      661 gccgattgaa accgaaaccg gcctgattgg cggttgtgcg gtggacgcca ctggcgtgcc
      721 gctgccggac gccacgctga agctggcgct ggaagccgac gccgtgctgc tgggcgcggt
      781 gggtggcccc aagtacgaca ccctgccgcg cgaacagcgc ccggaacgcg gcctgctgaa
      841 aatccgcaaa gaaatgaacg tgttcgccaa cctgcgcccg gcgattctct accccgagct
      901 ggccaacgcc tcgtcgctga agccggaagt ggtggccggc ctggacatca tgatcatccg
      961 cgaactggtg ggcgacatct actttggcga gccgcgcggc attgaagtgc gcaacggcga
     1021 aaaagtcggc ttcaacacca tgatttacag cgaaagcgaa atccgccgca tcgctcacgt
     1081 ggcgttcaag accgcgcaaa agcgcagcaa aaagctgtgc tcggtggaca aaatgaacgt
     1141 gctggaatcc acccagctgt ggcgcgacgt ggtcaccgaa gtggccgccg agtacccgga
     1201 cgttgagctg tcgcacatgc tggtggacaa cgccgccatg caactggtaa aaaacccgaa
     1261 acagttcgat gtgatggtca ctggcaatat ttttggcgac atcctgtcgg acgaagcctc
     1321 gatgctcacc ggctcgatcg gcatgctgcc gtccgcctcg ctggacgcca acggcaaggg
     1381 cctgtacgaa ccgattcatg gctccgcccc ggacatcgcc ggcaagggcc tggccaatcc
     1441 gctggccacc atcctgtcgg tggcgatgat gctgcgctac acctttaacc aggacgccgc
     1501 cgccgaccgc atcgaagccg cagtgaaatc ggtgctggcg caaggcctgc gcaccgccga
     1561 catctacgaa gacggcgcca cccgcgtcgg caccgtggaa atgggcgatg cggtgatcaa
     1621 ggcgctgacc gcctgattgc cggcgcagta caaagcgaga caacaccccc cacttgccgc
     1681 ctgcgggcgg catacagaat gcacaactaa aaaagtaagg aaatcatcat gttgaaagta
     1741 ggtctggttg gttggcgcgg catggtcggc tcggtgctga tgcagcgcat ggtcgaagaa
     1801 aacgactttg cccacatcga tccggtgtat ttcaccacct ccaaccccgg cggcgccgcg
     1861 ccgagctttg gcggcaaaac cgccccggcc ctgaaggacg ccaaaaacct ggacgagctc
     1921 aaggcatgtg atgtgatcat cacctgccag ggtggcgact acaccaccga agtctacggc
     1981 ccgctgcgcg ccgccggctg gaacggctac tggatcgacg ccgccagcac cctgcgcatg
     2041 gaagacgaag ccgtcatcat cctcgacccg gtcaatatga acgtgatcaa ggactcgctg
     2101 gccaagggcg gcaagacctt tgtcggcggc aactgtaccg tcagcctgat gctgatggcg
     2161 ctgggcggcc tgttccagaa cgatctggtg gactgggcca cctcgatgac ctaccaagcg
     2221 gcatcgggcg ccggcgccaa caatatgcgc gaactgctca agggcatggg cgcagtcaac
     2281 gccgccgtgg ccgccgagct ggccgacaag aacagcgcca tcctggacat cgaccgcaaa
     2341 gtggccgagt tcatccgctc ggacgccaac ccgaccgaat actttggcgt gccgctggcc
     2401 ggcagcctga ttccgtggat cgacaaagac ctgggcaacg gccaatccaa ggaagaatgg
     2461 aaaggcggcg tggaaaccaa caagatcctg ggccgcagcg acagcccgat cgtcatcgac
     2521 ggcctgtgcg tgcgcatcgg cgccatgcgc tgccacagcc aggcgctgac catcaagctc
     2581 aagcaagacc tgccggtggc cgaaatcgaa gccctgctgg ccaaggccaa cgactgggtg
     2641 aaagtggtgc ccaaccagcg cgaagcctcg atgaaagacc tcaccccggc cgccgtcacc
     2701 ggcacgctga ccgtcccggt cggccgcatc cgcaagctgg gcatgggcgg cgaatacatc
     2761 agcgccttca cctgcggcga ccaactgctg tggggcgccg ccgaaccgct gcgccgcatg
     2821 ctgcgcatcg tgctggacaa gtaacaagca aggcgctgcc acgcagggca tgatgaccca
     2881 agcgccgctc acaagccttt ggcgtggcga gcggcgcttg gcccggcgat ccggccaaac
     2941 cctccacact ctccagcctg ttgtagccgg caacagcgtc agcagcacct gccatcaaat
     3001 catcaggctg gcatcgtaag tgctgtgcaa gccctgcaga aaatcactgc tccccccaga
     3061 ttggcgccgc tggccacatc tacccctcct gcaggattaa aaatcagcac cgagtgtctt
     3121 ggcgcataga acgcaggctc ggcacccagc tgaaacccgc ccgcctgacc tcgccaacgt
     3181 aaggcatgtg cgtgaaataa aaacgctaat ccatcgcgcc agaccggttt tgtctgcggc
     3241 cctcactgcg tcgatttgcc tgttcaggcc acggcatgcc tcacccggtt gccgtgcggg
     3301 gtgaactgca ccaaattaga gccgctcata aaacccagcg aagcggtcct ggctaggcgt
     3361 gtgaccgcag acagtacaag cagtacggca aagtcacaca acaacgccag ggggttttgt
     3421 gagcggctct tcgtgcgatg cgtggcagaa tcagt
//
LOCUS       DAOPYX010000316         3442 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_36939_length_3442_cov_4.397992, whole genome shotgun sequence.
ACCESSION   DAOPYX010000316 DAOPYX010000000
VERSION     DAOPYX010000316.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 3442)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3442)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3442
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36939_length_3442_cov_4.397992"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(129..2411)
                     /locus_tag="PKN55_12730"
     CDS             complement(129..2411)
                     /locus_tag="PKN55_12730"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNX72156.1"
                     /translation="MPSLSSPVRPLTLAVCAALAAVSAFADEARLGEVLVSSGRDSAA
                     PVNLPASQAGMSAAQLAAAVNVINTEDVVKYLPSVQVRKRYIGDRNSIIATRTSGQTS
                     SARSLVYADGVLLSNLLGNRYDFPPRWNMVSADEISRVDMVYGPFAAEFPGNSVGSVV
                     LLNTRAPDKFEAHVKAQAFREDFSLYNSQQHYDGNKLEAGLGGRVGALAWQLDASHLD
                     SHGHPMSFAANPSRYTGSAAATAVSGALRDTDPYGASRLLLGATSVDHTTQDSARAKL
                     TWDITPTLRASYQLGYWQNRSDSTVDSYLRNAAGEAVYSGIVSIDGQKYTLGTLFAPS
                     QRQEAHLSNALTLASHSGGEWDWQAVASDYRYLKDHSRAPSVAIANPRQGGAGRITLM
                     DGTGWQTFDLKADWRPGGSRASEHQLRFGYHYDRYTLDSRTYATNDWLSGVQGAQTDA
                     FAGHTSTHALFVQDTWRFLPDWRLTPGLRYERWQAWGGRTLTAGKTFNHAERDNNYWS
                     PKLALSHDLNPDLTLRAAWGRAWRMPTVSELFQGSAAGSNSIVNNDPTLRPERGDTLE
                     LAAEYALGANGQLRLSAFQESLRDALLSQTNTTVNPNITNIQNIDRVRTRGLELAAKA
                     SDVGLRGLDLSGSLTWAHSRVLANSKNPTSEGNAYPGIPTWRATLLATWRQNEALSYS
                     LGARYSGKQAYDINNTATYDASQYGVNSRYLLVDARVQYKLNKQFTVSAGVDNLNNDK
                     SFAYHPMPQRTWHAEVKFDY"
     gene            2603..3253
                     /locus_tag="PKN55_12735"
     CDS             2603..3253
                     /locus_tag="PKN55_12735"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HNX72157.1"
                     /translation="MGHTLRDHAPIAVLAVCAHGAALAALWSGSPAPATQAPRALPMQ
                     VRMLAASPAATPAPAAKPMVKPKPAPTPPKAARPMPLAAPRAPASPTAPSAASAPSAV
                     PAPAAAAPAHPAAVADAAPTAPRFDADYLHNPAPAYPAMARRNGEEGRVQLSVRVSAQ
                     GRAEQVEVRHSSGYARLDEAALSTVRQWRFVPARRGEEAVAASVLVPIVFRLENAS"
BASE COUNT          494 a         1071 c         1230 g          647 t
ORIGIN      
        1 aagcgcaacg ccttgtgcag gccaatctgg cgttttgaca tatgacgccg gttaaatgga
       61 cacactacgt aatccaaata gacgcccgcc atccggcgcc gctgccgggc caggcgagcg
      121 cggcggggtc aataatcaaa cttcacttct gcgtgccagg tgcgttgcgg catcgggtgg
      181 taggcaaagc tcttgtcgtt gttgaggttg tccacgccgg ccgagacggt gaattgcttg
      241 ttcagcttgt attgcacgcg ggcgtccacc agcaggtagc ggctgttgac gccgtattgg
      301 ctggcgtcgt aggtggcggt gttgttgatg tcgtaggctt gtttgccgct gtagcgcgca
      361 cccaggctgt agctgagcgc ttcgttctgc cgccaggtgg ccagcagggt ggcgcgccag
      421 gtggggatgc cggggtaggc gttgccttcc gaagtcgggt ttttgctgtt ggccagcacg
      481 cgtgagtgcg cccaggtcag gctgccgctc aggtccaggc cgcgcaggcc gacgtcgctg
      541 gctttggcgg ccagttccag gccacgggtg cgcacgcggt cgatgttctg gatattggtg
      601 atattggggt tgaccgtggt gttggtctgc gacagcagcg cgtcgcgcag ggattcctga
      661 aaggcgctca gccgcagttg gccgttggcg cccagcgcgt attcggcggc cagctccagc
      721 gtgtcgccgc gttccgggcg cagcgtgggg tcgttgttga cgatgctgtt gctgccggcg
      781 gcgctgccct ggaacagctc ggacacggtc ggcatgcgcc aggcgcggcc ccaggcggcg
      841 cgcagggtca ggtcggggtt gaggtcgtgg ctgagcgcca gcttgggcga ccagtaattg
      901 ttgtcgcgct cggcgtggtt gaatgttttg ccggcggtca gcgtgcgtcc gccccaggct
      961 tgccagcgtt cgtagcgcag gccgggggtc agccgccagt cgggcaaaaa gcgccaggtg
     1021 tcttgcacaa acagcgcgtg ggtgctggtg tggccggcaa aggcgtcggt ttgcgcgccc
     1081 tgtacgccgc tgagccagtc attggtggcg taggtgcggc tgtccagggt gtagcggtcg
     1141 tagtggtagc caaagcgcag ttggtgttcg ctggcgcggc tgccgcctgg ccgccagtcg
     1201 gccttgaggt caaaggtttg ccagccggtg ccgtccatca gggtgatgcg cccggcgccg
     1261 ccctggcgcg ggttggcgat ggccacgctg ggcgcgcggc tgtggtcttt cagatagcgg
     1321 taatcgctgg ccaccgcctg ccaatcccat tcgccgccgc tgtggctggc cagggtcagc
     1381 gcattgctca ggtgggcttc ttggcgctgg ctgggggcaa acagggtgcc gagggtgtat
     1441 ttctggccgt cgatgctgac aatcccgcta tacaccgcct cgccggcggc attgcgcaga
     1501 tagctgtcca ccgtcgaatc cgagcggttt tgccagtagc ccagctgata gctggcgcgc
     1561 agggtggggg tgatgtccca ggtgagtttg gcgcgggcgc tgtcttgcgt ggtgtgatcg
     1621 acgctggtgg cgcccagcag caagcggctg gcgccgtagg ggtcggtgtc gcgcagcgcg
     1681 ccgctcacgg cggtggcggc ggcgctgccg gtgtagcggc tggggttggc ggcaaagctc
     1741 atcgggtggc cgtggctgtc cagatggctg gcgtccagct gccaggccag cgcgccgacg
     1801 cggccgccca ggccggcttc cagtttattg ccgtcgtaat gctgctggct gttgtacagc
     1861 gagaaatcct cacggaaggc ctgcgccttg acgtgggctt caaatttgtc tggcgcgcgg
     1921 gtgttcagca gcaccaccga gcctaccgag ttgccgggaa attctgcggc aaacgggcca
     1981 tacaccatgt ccacccggct gatttcgtcg gccgacacca tattccagcg cggcggaaag
     2041 tcatagcggt tgcccagcaa attggacagc aacacgccgt cggcatacac cagcgagcgc
     2101 gcgctgctgg tctgaccgga ggtgcgggtg gcgatgatgc tgttgcggtc gccgatatag
     2161 cgtttgcgca cctgcacgct gggcaggtat ttcaccacgt cctcggtgtt gatcacattc
     2221 accgcggcgg caagctgcgc ggcgctcatg ccggcctgcg acgcgggcag gttgaccggc
     2281 gcggcgctgt cgcggccgct gctgaccagc acttcgccca gccgggcttc gtcggcgaat
     2341 gcggaaacag cagcgagcgc ggcgcacacc gccagggtca gcgggcgcac gggcgaggag
     2401 agagagggca tggcagggct ccgggtaaat tatatttttt tgtaaatcaa tgttttgccg
     2461 ccaccacgca atgcaggtgg ggatgagtcg gccggcagta tggacacatc gcgcacgggc
     2521 gggaatgcgg cattttgtcg cacgacaagc cggaatccgg ctgctatggt ggcggccttt
     2581 gccagttttg agggacgacg ccatgggcca caccttgcgt gatcacgcgc cgattgccgt
     2641 cttggcggtg tgcgcgcatg gcgcggcctt ggccgccttg tggagcggct cgcccgcacc
     2701 agccacgcaa gcgccgcgcg ccttgccgat gcaggtgcgc atgctggccg cctcccctgc
     2761 ggcgacgccc gccccggcgg ccaagcccat ggtcaaaccc aaacctgcac ccactccgcc
     2821 caaggcggcg cggccgatgc cgctggcggc gccgcgtgcg ccagccagcc ccaccgcgcc
     2881 cagcgccgcc agcgcgccaa gcgccgttcc tgctcccgcc gccgccgcgc cagcccatcc
     2941 ggccgccgtc gccgacgccg ccccgacggc gccgcgcttt gacgccgact acctgcacaa
     3001 cccggcgccg gcctacccgg ccatggcgcg gcgcaatggt gaagaaggcc gggtgcagct
     3061 cagcgtgcgc gtcagcgccc aagggcgggc cgaacaggtg gaggtgcgcc acagcagcgg
     3121 ctatgcgcgc ctggacgagg ccgcgctgtc caccgtgcgc cagtggcgct ttgtgccggc
     3181 gcggcgcggc gaagaagccg tggccgccag cgtgctggtg ccgattgtgt ttcggctgga
     3241 gaacgcatcc tgagcgccgc tcagccaact cgcctgacgt gctggtgcgg ccttgccgta
     3301 tgccttcctt gcctgaccga cacacgcctg gccagaaagg ctttgcagcg tgttgtgcgt
     3361 ggtgcgctgt cttgtcgagc caaatgcatg gccgtttttg ttttcagtta cccgtaagga
     3421 aatctgcatg aatgcttctc tt
//
LOCUS       DAOPYX010000317         3398 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_37615_length_3398_cov_12.182770, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000317 DAOPYX010000000
VERSION     DAOPYX010000317.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 3398)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3398)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3398
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37615_length_3398_cov_12.182770"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..158
                     /locus_tag="PKN55_12740"
     CDS             <1..158
                     /locus_tag="PKN55_12740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019920373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HNX72158.1"
                     /translation="MGERNLSSFDRSIDVRISRLRRKLEDDPQNPKLIKTVYGAGYLF
                     ACAVVWR"
     gene            complement(190..654)
                     /locus_tag="PKN55_12745"
     CDS             complement(190..654)
                     /locus_tag="PKN55_12745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX72159.1"
                     /translation="MSTELFAGKKPRRASSPLRAPFRADSAELRRQAANMLHAFAERL
                     QNELARQGILPPPAIELWLDVQQQFCVRDNHPYADDILAVLETPSYYLHFKNLETLYE
                     LLECANSGALAQDSHLHIGLSSAGPVAFLCVRCDRSLAWRPRNRPCGKDGSG"
     gene            complement(865..3396)
                     /gene="mutS"
                     /locus_tag="PKN55_12750"
     CDS             complement(865..3396)
                     /gene="mutS"
                     /locus_tag="PKN55_12750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011137208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HNX72160.1"
                     /translation="MKSQHTDKLLFYRMGDFYELFYDDAEKAARLLDITLTTRGQSAG
                     APVRMAGIPFHAVEQYLAKLVKLGESAAICEQVGDPATSKGPVERQVVRVVTPGTLTD
                     AALLDDKTDNRLLALVFQQGVLGIAWLSLASGEFVVQETRAEHCLSELERLRPAEILV
                     ADNARLAVLEQVRAPIKRLPPWQFERASASERLARHFAVQDLAGFGAQDLGPAIGAAG
                     ALLEYARATQGSQLPHLDTLRVETSSQYIQLDAATRRNLELTETIRGEAAPTLFSLLD
                     QCATSLGSRLLRHWLHHPLRDHARIRARLDAVEALLPAHEPVLAALRPVADIERIASR
                     IALRSARPRDLSALRDSLLALPALHPLLADLNTPLLDQLAGQIPAQSPVAALLAQAVA
                     PEPASQLRDGGVLADGYDAELDQLRALQTDAGSYLLELEAREKARTGISTLKVGFNAV
                     HGFYIEVSKAQDAKVPDDYRRRQTLKNAERYITPELKTFEDKALSAKDRALAREKALY
                     EALLEQLLPHISDLRLTAQAVAGLDVLATFARHAASRQYVKPTLGQAPALDIRAGRHP
                     VVEAQVDRFIANDCQLGAARKLLLITGPNMGGKSTYMRQTALLTLLAHCGGFVPADSA
                     HFGPIDRIFTRIGASDDLAGGRSTFMVEMTETANILNNASEQALVLMDEVGRGTSTFD
                     GLALAMAIARALIEKNRSYTLFATHYFEMTRLAQDYPSVANVHLSAVEHQDKIVFLHH
                     VEDGPASQSYGLQVAALAGVPGTVIRQARRYLAQLENQAASQHAQPDLFAAPLPEPIA
                     EPDPLLGQLAGLDIDALSPREALALLYDMREQARNALGNGGNLPL"
BASE COUNT          556 a         1088 c         1157 g          597 t
ORIGIN      
        1 tgatgggtga acgcaattta tcctcgttcg accgcagcat cgacgtgcgc atctcccgcc
       61 tgcgccgcaa actggaagac gacccacaaa accccaaact gatcaaaacc gtgtacggcg
      121 ccgggtattt gtttgcctgt gcggtggtgt ggcggtaaac gcacgttcag cataattgcc
      181 ccgcccatct catcccgagc catccttgcc gcagggtcga ttgcgcgggc gccaggccag
      241 gctgcggtcg cagcgcacgc acaaaaacgc caccggcccg gcgctgctca ggccgatgtg
      301 tagatggcta tcttgcgcca gcgcgccgct gttggcgcat tccagtaatt cgtagagtgt
      361 ttccaggttt ttgaagtgca ggtaataaga cggggtttcc agcacagcca ggatatcatc
      421 ggcatagggg tggttgtcgc gcacgcaaaa ttgctgctgt acatccagcc agagttcgat
      481 ggcaggtgga ggcaggatgc cctggcgggc gagttcattt tgcaggcgct cggcaaacgc
      541 gtgcagcata ttggcggctt ggcggcgtag ttcggcgctg tcagcgcgaa acggcgcgcg
      601 cagagggctg ctggcgcggc ggggtttttt gccggcaaaa agctcggtgg acatggccag
      661 tctccttggg cggcggcggt gtgctgcgtg cacgcgcctg acaagggcgc cgtaccgcag
      721 ttcgcgcaat gagtaaatgc gacgcatctt caccacacag gcgaaaggcg gcaggtgcgc
      781 gcaaaacaat accccgaccg tgccgagcag ccggggcggc gtcaggcagg tgatgcgtga
      841 tgacgcacgc gcctgctcac cgtgttatag cggcagattt ccgccattgc caagcgcgtt
      901 gcgcgcctgc tcccgcatgt catacaacag cgccagcgct tcgcgcgggc tgagcgcgtc
      961 gatatccagc ccagccagtt gccccagcag cgggtcgggc tcggcgatgg gctcgggcag
     1021 cggcgcggca aacagatcag gctgggcgtg ctggctggcg gcctggtttt ccagctgggc
     1081 cagatagcgg cgcgcctggc ggatcaccgt gccgggcacg ccggccagcg ccgccacctg
     1141 caagccgtag ctctggctgg ccgggccgtc ttccacatgg tgcaaaaaga caatcttgtc
     1201 ctgatgttca acggcggaca ggtgcacatt ggccacgctg gggtaatcct gcgccagccg
     1261 ggtcatttca aagtaatggg tggcgaacag cgtgtaactg cggttctttt cgatcaacgc
     1321 acgggcaatg gccatggcca gcgccaggcc gtcaaaggtg gaagtgccgc gccccacttc
     1381 gtccatcagc accagcgcct gctcgctggc gttgttgaga atattggcgg tttcggtcat
     1441 ctccaccata aaggtcgagc gtccgccggc caggtcgtcg ctggcgccga tgcgggtaaa
     1501 aatgcggtca atcgggccaa agtgggcgct gtcggccggc acaaagccgc cgcagtgcgc
     1561 cagcagggtg agcagcgcgg tctggcgcat ataggtggat ttaccgccca tattcgggcc
     1621 ggtgatcagc agcagcttgc gcgccgcgcc cagctggcag tcgttggcga taaagcgatc
     1681 gacctgggct tccaccaccg gatggcggcc ggcgcggatg tccagcgccg gggcctggcc
     1741 cagcgtgggt ttgacgtact gacggctggc ggcgtggcgg gcaaaggtgg ccagcacgtc
     1801 cagcccggcc accgcctgcg cggtcaggcg caggtcgctg atgtgcggca gcagttgctc
     1861 cagcagcgcc tcgtacagcg ctttttcgcg cgccagcgcg cggtctttgg ccgatagcgc
     1921 cttgtcttca aaggttttca gctcgggggt gatgtagcgc tcggcgtttt tcagggtttg
     1981 ccgacggcgg taatcgtccg gcaccttggc gtcttgcgcc ttgctgacct caatataaaa
     2041 gccatgcacg gcgttaaagc ccactttcag cgtgctgatg ccggtgcggg ctttttcgcg
     2101 ggcttccagc tccagcagat aactgccggc gtcggtttgc agcgcgcgca gttggtccag
     2161 ctcggcgtcg tagccgtcgg ccagcacgcc gccatcgcgc agctggctgg ccggctcggg
     2221 cgccaccgcc tgcgccagca gcgccgccac cggcgattgc gccggaattt gcccggccag
     2281 ctggtcaagc agcggggtat tcaggtcggc cagcagcggg tgcagcgccg gcagcgccag
     2341 caggctgtcg cgcagcgccg acaaatcgcg cgggcgggcg ctgcgcaggg caatgcgcga
     2401 ggcaatccgc tcgatgtcgg ccaccgggcg cagcgccgcc agcaccggct cgtgtgccgg
     2461 cagcagcgct tccaccgcgt ccagccgggc gcggatgcgg gcgtggtcgc gcagcgggtg
     2521 gtgcagccag tggcgcagca gccggctgcc caggctggtg gcgcattggt ccagcaagga
     2581 gaacaaggtc ggcgcggcct cgccgcgaat ggtttcggtc agctccagat tgcgccgggt
     2641 ggcggcgtcc agctggatgt actggctgct ggtttccacc cgcagggtgt ccagatgcgg
     2701 cagctgcgag ccttgcgtgg cgcgggcgta ttccagcagc gcgcccgccg cgccgatggc
     2761 cgggccgagg tcttgcgcgc caaagccggc cagatcctgc acggcaaaat gccgcgccag
     2821 ccgctcgctg gcgctggcgc gctcgaactg ccacggcggc aggcgtttga tcggcgcgcg
     2881 cacctgctcc agcaccgcca gcctggcgtt gtccgccacc aggatttccg ccgggcgcag
     2941 ccgctccagc tcgctcaggc agtgttcggc gcgggtttcc tgcaccacaa actcaccact
     3001 ggccagtgac agccaggcaa tgcccagcac gccttgctga aacaccagcg ccagcaggcg
     3061 gttgtcggtt ttgtcgtcca gcagggcggc gtcggtcagc gtgccggggg tgaccacgcg
     3121 caccacctga cgctccaccg gccccttgct ggtggccgga tcgcccactt gttcgcaaat
     3181 cgccgccgat tcgcccagct tgaccagctt ggccaggtat tgctcgacag catgaaacgg
     3241 aatgccggcc atgcgcaccg gcgcgccggc cgactggccg cgcgtggtca gcgtgatgtc
     3301 gagcaagcgg gcggcttttt cggcgtcgtc gtaaaacagc tcgtaaaaat cgcccatccg
     3361 gtaaaacagc agtttgtcgg tgtgctggct tttcagcg
//
LOCUS       DAOPYX010000318         3396 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_37650_length_3396_cov_9.538761, whole genome shotgun sequence.
ACCESSION   DAOPYX010000318 DAOPYX010000000
VERSION     DAOPYX010000318.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 3396)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3396)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3396
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37650_length_3396_cov_9.538761"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1433)
                     /locus_tag="PKN55_12755"
     CDS             complement(<1..1433)
                     /locus_tag="PKN55_12755"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNX72161.1"
                     /translation="MMRPNPADWLAFHAHTRPQARALAAPAEGVLYDYASLHARVERL
                     AGGLAARGVTAGARLAVLALNHSDTLCLTLACARLGAMLLPLNWRLTASELQYQLDDA
                     EPAWLWHDDAHAALAGQLSGPTCAPLATLDDWLAAPLHPTPDIDPETPLLLLYTSGTT
                     GRPKGALLSQRMLAANADNTALSWDLSGADSSVAHAPFFHTGGLNVLTLPLLQRGGFS
                     VLLPRFDPGQVLSAIVNWRLSVLFAVPTMFQMLADHPDFAAAELGSLRFCISGGAPCP
                     IPLIERWAARGLTFKQGYGLTEAGVNCLTLPEAYAVSHRGSVGFANWGTQTRIVDEAG
                     QDVAVDQVGELWLAGPTLCSGYWRRPDASAELFAGRWLKTGDLFRRDAAGFHYVVDRK
                     KDMYISGGENVYPAEVEAAIQRLPGVRAAAVVGVADARWGEVGHAFVEMTAGLTLDID
                     ALNAHLASCLARFKRPQRIEQLDALPLT"
     gene            1735..2526
                     /locus_tag="PKN55_12760"
     CDS             1735..2526
                     /locus_tag="PKN55_12760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014087786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNX72162.1"
                     /translation="MRDIIHFSHANGFPAPVYRQLFAELSPDYDMGYLDMIGHDPRYP
                     VTDSWPHLVEETLHTLQARYTQPVIGVGHSLGGVITLLAALRRPQAFRAVVLLDAPLF
                     NPWRGRLIWLGKLLGQTERITPAGAALRRRAHWPDADSAVQHFAGKALFAEFSPQALR
                     DYIEHGADASEHGVRLRFQPQIEGRIFATLPHSFAAYRGRLAVPGAYVACSRHEVMRP
                     SDVRFACRRFGLKLYEHPGRHLFPLERPRETAAQLRQIIGELCGG"
     gene            complement(2628..3230)
                     /locus_tag="PKN55_12765"
     CDS             complement(2628..3230)
                     /locus_tag="PKN55_12765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014088242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNX72163.1"
                     /translation="MDALFHPLPTSQLPVNTDLLHLDERPARPVTYDSPALEVMTDLR
                     VLDPVSIAEDATLDAAHTLMVSRAIRLLFVTDGDHHLTGVITATDVLGERPMQCIHQD
                     GKRHTEILVRDVMTPRTQLDTLQLKDVSHAKVGQLIATMKQLGRQHTLVVEHNAHTGH
                     HELCGLFSTSHIARRLGVPLHFIRVPQTFSEIQHTLTHPA"
BASE COUNT          599 a         1080 c         1141 g          576 t
ORIGIN      
        1 gtcagcggca gcgcgtcgag ttgctcaatg cgctgcgggc gcttgaagcg cgccaggcaa
       61 ctggccaagt gggcattcaa ggcgtcgatg tccagggtca ggccggcggt catttcgaca
      121 aaagcatggc ccacctcgcc ccagcgcgca tcggccacgc ccaccacggc ggcggcgcgc
      181 acgccgggga ggcgctggat ggcggcttcc acctcggccg ggtaaacatt ctcgccgccg
      241 ctgatataca tgtctttttt gcggtccacc acataatgaa aaccggcggc gtcgcggcgg
      301 aacagatcac cggttttcag ccagcggccg gcaaacagtt cggcgctggc gtctggccgc
      361 cgccagtagc cagaacacaa ggtcggccca gccaaccaca gctcgcccac ctgatccacg
      421 gccacatcct ggccggcctc atcgacaatg cgcgtttgcg tgccccagtt ggcaaagccc
      481 accgagccgc gatgcgacac cgcgtaggct tctggcaggg tcaggcagtt caccccggct
      541 tcggtcaggc cgtagccttg cttgaaggtc agcccacgcg ctgcccagcg ctcgatcagc
      601 ggaatcgggc agggtgcgcc gcccgagatg caaaagcgca ggctgcccag ttctgctgcg
      661 gcaaagtcgg ggtggtcggc cagcatctgg aacatggtcg gcacggcaaa cagcacgctc
      721 aagcgccaat taacgatggc gctcagcacc tggccggggt caaaacgcgg cagcaacacg
      781 ctgaagccgc cgcgctgcaa gagcggcagg gtcagcacat tcagcccgcc agtatgaaaa
      841 aacggcgcat gcgccacgct gctgtccgcg ccggacagat cccaggacag cgcggtgttg
      901 tcagcattgg ccgccagcat gcgttgcgac agcaaggcgc ccttgggccg gccggtggtg
      961 cccgaggtgt acagcagcaa gagcggggtt tccgggtcga tgtctggcgt ggggtgcagt
     1021 ggcgcggcca gccagtcgtc cagcgtggcc agcggcgcgc atgtcgggcc gcttagctga
     1081 ccggccagcg cggcgtgggc atcgtcatgc cacagccagg ctggttcggc gtcgtcgagc
     1141 tggtattgca gctcgctggc ggtcagccgc cagttcagcg gcagcagcat ggcgcccagc
     1201 cgggcgcagg ccagcgtcag gcacagggtg tcgctgtgat tcagcgccag caccgccagc
     1261 cgcgcgccgg ccgtcacccc gcgcgcagcc aggccgccgg ccaggcgctc gacgcgggca
     1321 tgcaggctgg cgtagtcata cagcaccccc tcggctggcg cagccagcgc gcgcgcctgc
     1381 gggcgggtgt gggcgtgaaa ggccagccag tcggcagggt tgggacgcat catgacagct
     1441 ccagaggcag cggcgggaga gcatgatgtt agcaagattt gccgccgcca tggcgggaac
     1501 tgcgttgagg tgtttacaat gctggcggag aaattaaaac aactgtttgc ttgcccagtg
     1561 tgacgacctt atcgccaggc caggccgaca cgggtcctga gtgcataagc gctgttcacc
     1621 ctcctcagcg cagaggcgca caaccgcaga cagtacaaaa aatacggcaa ggccaggcgg
     1681 gtgttgtgcg ccgcgccaag cgcaatcggc cgccagacag ggagctcaca aagcatgcgc
     1741 gacatcattc atttttctca cgccaatggg tttccggcgc cggtgtaccg ccagctgttt
     1801 gctgagctat cgccggatta cgacatgggc tatctggaca tgatcggcca cgatccgcgc
     1861 tatccggtaa ccgacagttg gccgcatctg gtggaagaaa ccctgcacac cctgcaagcg
     1921 cgttacaccc agccggtgat tggcgtgggg cattcgctgg gcggggtgat caccctgctg
     1981 gcggcgctgc gccggccgca ggcgtttcgc gccgtggtgt tgctggatgc gccgctgttc
     2041 aacccctggc gcgggcgctt gatctggctg ggcaaattgc tggggcaaac cgagcgcatc
     2101 accccggccg gcgcggcctt gcgccgccgc gcgcactggc cggacgccga tagcgcggtg
     2161 cagcattttg ccggcaaggc gctgtttgcc gagttttcgc cgcaggcgct gcgcgattat
     2221 attgaacacg gcgccgacgc cagcgagcac ggcgtgcgcc tgcgctttca gccgcagatc
     2281 gaaggccgga ttttcgccac cctgccgcac agctttgccg cctatcgcgg ccggctggcg
     2341 gtgcccggcg cctatgtggc gtgcagccgc cacgaggtga tgcgccccag cgatgtgcgc
     2401 tttgcctgcc ggcgttttgg cctgaaatta tacgaacacc ccggccgtca tttgtttccg
     2461 ctggagcgcc cgcgcgaaac cgccgcgcag ttgcgccaga tcatcggcga actgtgtggc
     2521 ggctgaacgc taaagcaggc gccaaacccg gcaaaatcaa aaaccgccat gcccactcgt
     2581 ctggaaatgg cggtgttgtc tgcatgcccc tggcgggcaa aacccgctca ggccgggtgg
     2641 gtcagcgtgt gctgaatttc ggaaaacgtc tgcggcacgc gaataaagtg cagcggcacg
     2701 cccaggcggc gggcgatgtg cgaggtggaa aacagcccgc acagctcgtg gtggccggtg
     2761 tgggcgttgt gctccaccac cagggtgtgc tgacggccca gctgtttcat ggtggcgatc
     2821 agctggccca ccttggcgtg gctgacgtct ttgagctgca aggtgtccag ctgggtgcgc
     2881 ggggtcatca cgtcgcgcac caggatttcc gtgtggcgct tgccatcctg atggatgcac
     2941 tgcatcggcc gctcgcccag cacgtcggtg gcggtgatca ccccggtcag gtgatggtcg
     3001 ccgtcggtga caaacagcag gcgaatggcg cggctgacca tcagcgtgtg cgcagcgtcc
     3061 agcgtggcgt cctcggcaat gctgaccgga tcaagcacgc gcaggtcggt catcacctcc
     3121 agtgccgggc tgtcgtaagt caccgggcgg gccggacgtt cgtccagatg caataaatcg
     3181 gtgttgacgg gcagttggct ggttggcagg gggtgaaaca gagcatccat gactgaatct
     3241 cctcattgtg cgcccgtggc gaatctcctg cgccgacggg ctgttatggt ggcgaaacac
     3301 gccggacatg gcctgaatga cgtgtttctg attccattct agcgcgcacg cgagaaattg
     3361 ctcgcctgtc gtgaagagca ctcgtttgca tggcaa
//
LOCUS       DAOPYX010000319         3337 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_38606_length_3337_cov_15.031993, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000319 DAOPYX010000000
VERSION     DAOPYX010000319.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 3337)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3337)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3337
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38606_length_3337_cov_15.031993"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(472..1164)
                     /locus_tag="PKN55_12770"
     CDS             complement(472..1164)
                     /locus_tag="PKN55_12770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003822263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HNX72164.1"
                     /translation="MKTLGVLGGMSWESTAVYYRLFNQGVRARLGGQHSAKLLLASVD
                     FAEIVARQHAGDWHGAGQQLAELAAGLQNAGADAILIATNTMHKVAEQVQAAIDVPLL
                     HIGDVVADALLAAGVRRAGLLGTRYTMEQPFLIDHLRQRGLDILVPDADARADVHRII
                     FDELCQGEVNNVSRETYQRIIQQLAADGAQAVILGCTEICLLLGPNDACLPLYDTAAL
                     HVDAGLNWALQP"
     gene            complement(1331..>3337)
                     /gene="parC"
                     /locus_tag="PKN55_12775"
     CDS             complement(1331..>3337)
                     /gene="parC"
                     /locus_tag="PKN55_12775"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011133630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HNX72165.1"
                     /translation="QDFVMRYPLIDGQGNFGSRDGDGAAAMRYTEARLTPIAELLLSE
                     IDHGTVDFVPNYDGAFEEPQLLPARLPMVLLNGASGIAVGLATEIPPHNLREVASAAV
                     ALLRQPELDLDALLAYIPGPDFPGGGQIITPPADIRTAYQSGRGSLRVRARWQIERLA
                     RNQWRVVVNELPPGTSAQKVLAEIEELTNPKVRPGKKALSAEQQNLKGLMLGQLDRVR
                     DESDQQQPVRLVFEPKSSRQDPDEFITLLLAQTSLEGNVPMNLVMLGLDGRPRQKDLK
                     SILSEWLDFRMHTVTRRLSHRLDKVNQRTHILEGRMTVFLHIEDVIHVIRESDEPKPE
                     LMRRFGLSDIQAEDILDIRLRQLARLEGFKLEKELDALRGEAGTLRHLLDNESARRDL
                     VAEEIRADAARYGDARRTEVKPAEKAVLTQTVADEPVTLIVSRNGWIRARSGHNVDVS
                     ALNFKDGDALGQVVETRTIHPVVALDSRGRSYTLACSDVPTGRGDGVPLSSLIDIQDK
                     ARVVAVLSAKDDSRYLVASSGGYGFIAQLKDMVSRVKAGKAFLTVEDQESALPPVPAP
                     DHASAHVLVVSQQARLLAFALDELKQMGRGRGLQLLALDDGDALTALGVVSSGRALLT
                     VQIPRSGRLDEVRLPLDEFIGKRGRRGKEAPKRWLVLGVRDAQG"
BASE COUNT          506 a         1036 c         1152 g          643 t
ORIGIN      
        1 ggcgggcgag atggcgcaaa actgcaactg gggttttcag atatgaacaa gttggcgggc
       61 ttgggtgtgt gtggggaggg tggggccgcg ctggcgcgcg gcataggctg ggggtggatt
      121 ttgggcgggg cttcgccccg cacccgaccc cttttctttg tctcgccaaa gaaaagggga
      181 aaagaaaggc gacccgggcg ccgcgccctt cgggtgccct gcgcttctcg aaagtcaggg
      241 ccgtcccgga atggcgcgtc ggctgtgccg ccgcttagcc gggccggaaa cctccctgac
      301 tttctgcgat gctcggcgcg tcacacgggg ggaacggcgg ctaggcgcga cgggctgtgt
      361 ctttgagctg ctcaccaaac cattctggcg ttgttgcgcg gccttgccgt actgcttgta
      421 ctgtctacgg ccggattttt tgccgccatg ctgccgcgtg cagctgaacg cttacggttg
      481 cagcgcccag ttcaggccgg cgtctacatg cagggcggcg gtgtcgtata gcggcaggca
      541 ggcgtcgttg gggcccagca gcaggcagat ttcggtgcaa cccagaatca ccgcttgcgc
      601 gccgtcggcg gcgagttgtt ggatgatgcg ctggtaggtt tcacgtgaaa cattgttgac
      661 ctcgccctga cagagttcgt caaagatgat gcgatgcacg tcggcgcggg cgtcggcgtc
      721 ggggacgagg atgtccaggc cgcgttggcg caggtggtca atcaggaacg gttgttccat
      781 ggtgtagcgg gtgcccagca ggccggcgcg gcgtacgccc gccgccagca gggcgtcggc
      841 caccacgtcg ccgatgtgca gcaagggcac gtcgatggcg gcttgcactt gctcggcaac
      901 tttgtgcatg gtgttggtgg cgatcaggat ggcgtcggcg ccggcgtttt gcagcccggc
      961 ggccagttcg gccagttgct ggccggcgcc gtgccagtcg ccggcgtgtt ggcgggcgac
     1021 gatctcggca aagtccacgc tggccagcag cagcttggcc gagtgctggc cgcccagccg
     1081 ggcgcgcacg ccttgattga acagccgata gtaaacggcg gtggattccc aactcatccc
     1141 gcccaacaca cccagggttt tcatggtgac gccttatgtg aattgcaatg gccacactgt
     1201 aaacgtcgct ggcggcactg cccagccaca gtgggtgtgg ggctgtgctg gcgcaaaaac
     1261 agcacagcgc cgaaaacggc gctgtgtggg gggaggtgaa gtttcggcgg tcagatgggt
     1321 ttggcggcgc ttagccctgg gcgtctctca cacccaacac cagccagcgc ttgggcgctt
     1381 ctttgccgcg tcggccgcgt ttgccgataa attcgtccag cggcaggcgc acttcatcca
     1441 ggcggccgga gcggggaatc tgcacggtca acagcgcgcg gccgctgctc accacgccca
     1501 gcgccgtcag cgcgtcgccg tcgtccagcg ccagcaattg caggccacgg ccacggccca
     1561 tttgcttgag ctcgtccagg gcaaaggcca gcaggcgcgc ctgctggctg accaccagca
     1621 catgcgcgct ggcgtggtct ggcgccggaa ccgggggcag tgcgctttct tggtcttcga
     1681 cggtcaggaa ggctttgccg gctttgactc ggctgaccat gtccttgagc tgggcaataa
     1741 agccataacc gccgctgctg gccaccagat agcggctgtc gtccttggcg gacagcaccg
     1801 ccaccacgcg ggccttgtcc tgaatgtcga tcagcgagct cagcggcacg ccgtcgccgc
     1861 gcccggtggg cacgtcgctg cacgccagcg tgtagctgcg gccacggctg tccagcgcca
     1921 ccactgggtg aatggtgcgg gtttccacca cctgacccag tgcgtcgcca tctttgaagt
     1981 tcagcgccga cacgtccaca ttgtggccgc tgcgcgcgcg aatccagcca ttgcgcgaga
     2041 caatcagcgt caccggctcg tcggcgacgg tttgcgtcag cacggctttt tcggcgggtt
     2101 tgacttcggt gcggcgggcg tcgccatagc gggcggcgtc ggcgcggatt tcttccgcca
     2161 ccaggtcgcg gcgggcggat tcattgtcca gcaggtgacg cagggttccg gcttcgccgc
     2221 gcagggcgtc cagttctttt tccagcttga agccttccag ccgcgccagc tggcgcaggc
     2281 ggatgtccag aatgtcttcg gcctggatgt cgctcaggcc aaagcggcgc atcagctcgg
     2341 gcttgggctc gtcggattcg cgaatgacgt ggatcacatc ctcgatatgc aggaacaccg
     2401 tcatgcggcc ttccaggatg tgggtgcgct ggtttacctt gtccagccgg tggctcagcc
     2461 ggcgggtgac ggtgtgcatg cggaaatcca gccattcgct gaggatggat ttcaggtctt
     2521 tctggcgcgg ccggccgtcc aggcccagca tcaccagatt catcggcaca ttgccttcca
     2581 ggctggtttg cgccagcagc agggtgatga attcgtccgg gtcttgccgg ctggatttgg
     2641 gttcgaacac cagccgcacc ggctgttgtt ggtcggattc gtcgcgcacc cggtccagct
     2701 ggcccagcat caggcccttg aggttttgtt gctcggccga cagcgctttt ttgccggggc
     2761 gcaccttggg gttggtcagc tcttcgattt ccgccagcac tttttgcgcc gacgtgcccg
     2821 gcggcagttc gttgaccacc acgcgccact gattgcgcgc cagccgttcg atctgccagc
     2881 gcgcccgcac gcgcaggctg ccgcgtccgc tttggtaggc ggtgcggatg tcggccggcg
     2941 gggtgatgat ctggccgccg ccaggaaaat ccgggccggg gatataggcc agcagcgcgt
     3001 ccaggtccag ctcgggctgg cgcagcagcg ccacggcggc gctggccacc tcgcgcagat
     3061 tgtgcggcgg gatttccgtg gccaggccga cggcaatgcc ggaggcgccg ttgagcagca
     3121 ccatcggcag ccgcgccggc aagagctgcg gctcttcaaa ggcgccgtcg tagttgggga
     3181 caaagtccac cgtaccgtgg tcgatttctg acagcagcaa ttcggcaatc ggcgttagcc
     3241 gggcttcggt gtagcgcatg gccgctgcgc cgtcgccgtc gcggctgcca aaattgccct
     3301 ggccgtcgat cagcgggtag cgcatgacaa aatcctg
//
LOCUS       DAOPYX010000320         3323 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_38858_length_3323_cov_6.425643, whole genome shotgun sequence.
ACCESSION   DAOPYX010000320 DAOPYX010000000
VERSION     DAOPYX010000320.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 3323)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3323)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3323
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38858_length_3323_cov_6.425643"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..226)
                     /locus_tag="PKN55_12780"
     CDS             complement(<1..226)
                     /locus_tag="PKN55_12780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="class I poly(R)-hydroxyalkanoic acid synthase"
                     /protein_id="HNX72166.1"
                     /translation="MSQDKQGPSLESFFSSISDANRKLMEQFVGNFAGMKLPDGELNP
                     FVSMWGAAAKSASQLVEMQTNLYQQQMNLWM"
     gene            complement(500..1396)
                     /locus_tag="PKN55_12785"
     CDS             complement(500..1396)
                     /locus_tag="PKN55_12785"
                     /inference="COORDINATES: protein motif:HMM:NF015509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HNX72167.1"
                     /translation="MLAIALILLPDFSLILLGLLLRRRFAYPAEFWGHLERLVYYLMF
                     PALLFRAVSRTELDIAPALDMFIAGFGFSVVGIVLGVLGRWLLKPDGRVFASCVQTGF
                     RFNTYIGLAVVDRLSGHAGIASFAVLIGVMVPWVNAVSVWFLARQGGASVWRELAKNP
                     LIVATVSGFIVSLFGLPLPGEVNHVLDLLAAAALPLGLLSVGAGLRAEGLRASLPLVN
                     YFTFVKLVAVPAAAWGLALLLGLKGLYFQSVLVLAALPTASSAYILAVRMGGDGRTVA
                     TIITVNVFVAMVTLPLWLGLAL"
     gene            complement(1532..2671)
                     /locus_tag="PKN55_12790"
     CDS             complement(1532..2671)
                     /locus_tag="PKN55_12790"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HNX72168.1"
                     /translation="MLFERITMKFDSLESLRGVAALLVALSHSSFLYGQQHVFIANAG
                     VFVDFFFVLSGFVMAFAYFDKVREGMPFSRFILLRFGRLYPLHLLTLLLWVPYVLAKG
                     YAFHVLHLGEMDPWVKNSPLRFVYNIFLVNSLGFDNDLGWNAPAWSISVEFYTYIVFF
                     FFVLLLRPVYRPVFSLILSVLSYAALVYVARDDPHTLLYTYKYGIFRCMGGFFLGMFV
                     YHLSQRSRIQLKAFPASLIEVALLLIACALIAESHHKAMQLPVFPVFAVIVYVLSIQD
                     VGVVSRFLKMKWMVLLGTLSYSIYMIHMLVFVLAANIWQYVLKLPVSSMTHREGGSPF
                     KVIDTPYADLINLGLIALVIVISYFVYHRFESIFRDSFRRLALRR"
BASE COUNT          934 a          976 c          863 g          550 t
ORIGIN      
        1 tcatccacag attcatctgc tgctggtaca gattggtctg catttccacc agttgcgatg
       61 cgctcttggc ggcggcgccc cacatgctga caaacgggtt gagctcgcca tccggcagct
      121 tcatgcccgc aaagttgccg acgaattgct ccatcagctt gcgattggcg tccgaaatgc
      181 tggagaaaaa cgactccagc gacgggcctt gcttatcttg actcatgacc tatcttcctc
      241 ttcttggtgg ttcctccgcg ccgggcgcgg attcgggggt atgtccgtcg tgtaacaccg
      301 cacccgggcg gctgcgtcgc cgggcgctgg atgattgaac ctgcgcgttc ggggtcttgt
      361 tatgcttgcc cctgtctgct tgtgtcagcg cacgaagcac cgatgacatt aacttatcac
      421 ggtttcgtga aaaattgttg cattgcgccc agggttttta cgtttgcgcg cgcaacgcgg
      481 ctggcgcgcc aaaacccgct cacaaagcca ggcccagcca cagcggcaag gtcaccatcg
      541 ccacaaacac attcaccgtg atgatggtgg ccaccgtacg gccatcgccg cccatgcgca
      601 ccgccagaat ataagccgaa gacgcagtcg gcaacgccgc cagcaccagt accgactgaa
      661 aatacaagcc cttgagcccc agcagcaggg ccagccccca cgccgccgcc ggcacggcca
      721 ccagtttgac aaaagtaaag taattcacca gcggcaaaga ggcgcgcagc ccttcggcgc
      781 gcagcccggc ccccaccgac agcaaaccca gcggcaaggc ggcggcggcc agcaagtcca
      841 gcacatgatt cacttcgccc ggcaaaggca ggccaaacag actgacgatg aagccgctga
      901 ccgtggcgac aatcagcgga tttttcgcca gctcgcgcca caccgacgcg ccgccctgac
      961 gggccagaaa ccacaccgac accgcgttta cccacggcac catcaccccg atcagcacgg
     1021 caaacgaggc aatgccggcg tgtccggaca agcgatccac caccgccagg ccaatatagg
     1081 tattgaagcg aaacccggtt tgcacgcagc tggcgaacac ccgtccgtcc ggcttgagca
     1141 gccagcgccc cagcacgccc aagacgatgc ccactacgct aaaaccaaag ccggcgataa
     1201 acatatccag cgccggggcg atgtccagtt cggtgcgcga caccgcgcgg aacagcaagg
     1261 ccggaaacat caggtaatac accaaccgct ccagatgccc ccagaactcg gccgggtagg
     1321 caaaacggcg gcgcagcagc aggccgagca gaatcagtga aaaatcaggc agcaggatca
     1381 gggcgatggc gagcatggaa aaaataagcc gggccatgac ggcccatgtc agacagaagc
     1441 tgaaaatgta cccgtgccag cgcgccatgg caaaacgccc gtacaggcaa acctgtgcgg
     1501 gcgtttgatg ggttcgcgct cgcggcggcg ctcagcggcg caaggccagg cggcggaagg
     1561 agtcgcgaaa gatcgactca aagcggtgat agacaaaata cgaaatcacg atcaccaggg
     1621 caatcaaacc caggttgatc agatccgcat aaggggtatc aatcaccttg aatggtgagc
     1681 cgccttcgcg gtgtgtcatc gacgacacag gcagttttag tacgtattgc cagatattgg
     1741 cagccagaac aaaaaccaac atgtgaatca tatagatcga ataagacaac gtacccagca
     1801 acaccatcca tttcattttc aaaaagcgac tgacgacgcc gacatcttga atggacagca
     1861 catacacaat gaccgcaaac accggaaaga caggtaactg catggcttta tggtgcgatt
     1921 cagcaatcag ggcacaggca atcagcaaca gcgccacttc aatcagggag gcggggaatg
     1981 ccttgagctg aatgcgcgag cgttgtgaca ggtgatagac aaacatcccc aggaaaaacc
     2041 cacccataca gcggaagatt ccgtatttat acgtatacaa cagtgtatgg ggatcgtctc
     2101 gggccacata caccagtgct gcataactca gcacggacag gatcagggaa aacaccggtc
     2161 tgtatacggg ccgcagcagc agcacaaaaa agaagaacac aatgtaggta taaaactcga
     2221 cactgatgct ccaggcgggg gcattccagc ccaggtcatt gtcaaaaccc agcgagttga
     2281 ccaagaaaat attgtagaca aaacgcagcg ggctgttctt cacccaggga tccatctcgc
     2341 ccagatgcag cacatgaaat gcatagccct tggccagcac gtaaggcacc catagcagca
     2401 gggtgagcag atgcaaggga tacagacggc caaaacgcag caggatgaat cgggaaaacg
     2461 gcatgccttc acgaactttg tcaaaatagg caaacgccat gacaaagcca gacagcacaa
     2521 agaaaaagtc aacaaacacg cccgcattgg cgataaagac atgctgctga ccatacaaga
     2581 aactggaatg gctcagcgca accagcaagg cggccacgcc gcgcaaagac tcaagtgaat
     2641 caaacttcat ggttatccgt tcaaacagca tcaacagaca ttctcaaaga agcgggtgtg
     2701 ttttttaccc acccacctaa agaatgtagc ctgaaaaaca atatttacat tggtcgaatg
     2761 tgcctgactc aaaaacccca ggcaatacca acgtattaaa tcttatacaa cccagcttca
     2821 ggcacggcat gatttgacaa gacaccatgt cagacttgcc ataacatgcg tcagcatgga
     2881 ctgcagcgaa aagcgggcta tcctagcatt aacttggctt aagaagccac tcggaatcaa
     2941 aatttctgcc acgaatttgt ggtaactcac agacaccaag taaatatacg tagcataccc
     3001 aacaacaggt gcaatagact gcattaagcg cggctcacaa accccagcaa agcgatcctg
     3061 gctaggcgcg cgacagcaga cagtacgggg acgtgcaatc acgccaggag ttttgtaagc
     3121 ggcgccaagt agagaaaagg cacacgagaa aaaccccgcc agccataggg gaatacggac
     3181 acgctgcaca ctggtgagcg aggcaaatag gtgtgcgctt atggttttgg ctaaatggga
     3241 aacccgcaca tacccgcgga aaactttctc acaacgccag cctctccggc gtgtgtcgct
     3301 tgcgtcctgc tgagcgtcgc tca
//
LOCUS       DAOPYX010000321         3314 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_39011_length_3314_cov_10.579626, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000321 DAOPYX010000000
VERSION     DAOPYX010000321.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 3314)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3314)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3314
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39011_length_3314_cov_10.579626"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..878
                     /gene="prpC"
                     /locus_tag="PKN55_12795"
     CDS             <1..878
                     /gene="prpC"
                     /locus_tag="PKN55_12795"
                     /EC_number="2.3.3.16"
                     /EC_number="2.3.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050440 - 2-methylcitrate synthase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2-methylcitrate synthase"
                     /protein_id="HNX72169.1"
                     /translation="EALPAASHPMDVMRTAVSVLGCALPEKDDHNAPGARDIADRLIA
                     SLGSALLYWYHFSTNGKRIDVETDDDSVGGHFLHLLHGEKPSELWVKAMHTSLILYAE
                     HEFNASTFTGRVIAGTGSDMYSAITGAIGALRGPKHGGANEVAFEVQKRYDSPDAAEA
                     DIKARVEKKEVVIGFGHPVYTISDPRNKVIKEVARELSSAAGDMKMFDIAERLESVMW
                     DIKKMFPNLDWFSAVSYHMMGVPTAMFTPLFVIARTTGWAAHVIEQREDGKIIRPSAN
                     YIGPDDLKFAPISER"
     gene            986..>3314
                     /gene="acnD"
                     /locus_tag="PKN55_12800"
     CDS             986..>3314
                     /gene="acnD"
                     /locus_tag="PKN55_12800"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012697779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047456 - 2-methylisocitrate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe/S-dependent 2-methylisocitrate dehydratase
                     AcnD"
                     /protein_id="HNX72170.1"
                     /translation="MNTAFRKPLPGAALDYYDTRAAVDAIAPGAYDTLPYTSRVLAEQ
                     LVRRCDPAALTDSLKQLIERKRDLDFPWYPARVVCHDILGQTALVDLAGLRDAIADQG
                     GDPSLVNPVVPTQLIVDHSLAVECGGFDPEAFEKNRAIEDRRNEDRFHFINWTRTAFK
                     NVDVIPAGNGIMHQINLEKMSPVIQARDGVAFPDTCVGTDSHTPHVDALGVIAIGVGG
                     LEAETVMLGHPSMMRLPDIVGVKLTGQRKPGITATDIVLALTEFLRKERVVGAYVEFF
                     GEGADSLSIGDRATISNMCPEYGATAAMFYIDQQTIDYLKLTGREPEQVALVENYAKL
                     TGLWAESLKTAQYERVLEFDLSSVVRNMAGPSNPHKRLPTSALQERGIADEAKLAAAR
                     AEEAQGLLPDGAVVIAAITSCTNTSNPRNVVAAALLAKKANALGLVRKPWVKTSFAPG
                     SKVAKLYLEEAGLLPELEKLGFGIVAYACTTCNGMSGALDPKIQQEIIDRDLYATAVL
                     SGNRNFDGRIHPYAKQAFLASPPLVVAYAIAGTVRFDIEQDVLAVVDGKEIRLKDLWP
                     SDEEIDAIVAAAVKPEQFKQIYIPMFDLGEAEQAESPLYNWRPMSTYIRRPPYWDTEG
                     VGALAANPRTLKGMRPLAVLPDNITTDHLSPSNAILASSAAGEYLAKMGLPEEDFNSY
                     ATHRGDHLTAMRATFANPQLVNEMAVVDGQVKKGSLARVEPDGKVLRMWEAIETYMDR
                     KQPLIIVAGADYGQGSSRDWAAKGVRLAGVEVIVAE"
BASE COUNT          636 a         1139 c         1018 g          521 t
ORIGIN      
        1 tggaagccct gccggccgcc agccacccga tggacgtgat gcgcaccgcc gtgtcggtgc
       61 tgggttgcgc cctgccggaa aaagacgacc acaacgcacc gggcgcgcgc gacatcgccg
      121 accgcctgat tgcctcgctg ggttcggccc tgctgtactg gtaccacttc agcaccaatg
      181 gcaagcgcat cgacgtggaa accgatgacg actccgtggg cggccacttc ctgcacctgc
      241 tgcacggcga aaagccgtcc gagctgtggg tgaaggccat gcacacctcg ctgattctgt
      301 acgcagaaca cgagttcaac gcctccacct tcaccggccg cgtcatcgcc ggcacgggct
      361 cggacatgta ctcggccatc accggcgcca tcggcgcgct gcgcggcccc aagcacggcg
      421 gcgccaacga agtggcgttt gaagtgcaaa agcgctacga ctcgccggac gcagccgaag
      481 ccgacatcaa ggcccgcgtc gagaaaaaag aagtggtgat tggctttggc cacccggtgt
      541 acaccatcag cgacccgcgc aacaaggtga tcaaggaagt cgcccgcgaa ctgtcttccg
      601 ccgctggcga catgaagatg ttcgacatcg ccgagcgtct ggaaagcgtg atgtgggaca
      661 tcaaaaagat gttcccgaac ctggactggt tcagcgccgt gtcctaccac atgatgggcg
      721 tgccgaccgc catgttcacc ccgctgttcg tgattgcccg caccaccggc tgggccgccc
      781 acgtgatcga gcagcgcgaa gacggcaaga tcatccgccc gagcgccaac tacattggcc
      841 cggacgatct gaagttcgcg ccgatcagcg agcgttgatc gactgaccca aggggagcgg
      901 gccgcctggc ggcggctccc cttttgcgcc acagagcctg ctcacaggcc cgtgccaacc
      961 gtcttcacct ggattttgtc acgtcatgaa cactgcattc cgcaagcccc tgcccggcgc
     1021 cgcgctggac tactacgaca cccgcgccgc ggtggacgcc atcgcccccg gcgcttacga
     1081 caccctgccc tacacctcgc gcgtgctggc cgaacagctg gtgcgccgct gcgacccggc
     1141 cgcgctgacc gattcgctca agcaactgat cgagcgcaag cgcgatctgg atttcccgtg
     1201 gtatccggcc cgtgtggtgt gccatgacat cctgggccaa accgcgctgg tggacctggc
     1261 cggcctgcgc gacgccatcg ccgaccaagg cggcgacccg tcgctggtca acccggtggt
     1321 gcccacccag ctgatcgtgg accactcgct ggccgtggag tgcggcggct tcgacccgga
     1381 agccttcgag aaaaaccgcg ccattgaaga ccgccgcaac gaagaccgct tccacttcat
     1441 caactggacg cgcaccgcgt tcaagaatgt ggacgtgatc ccggccggca acggcatcat
     1501 gcaccagatc aatctggaaa aaatgtcccc ggtgattcag gcgcgcgacg gcgtggcctt
     1561 cccggacacc tgcgtgggca ctgacagcca caccccgcac gtggacgcgc tgggcgtgat
     1621 cgccatcggc gtgggcggcc tggaagccga aaccgtgatg ctgggccatc cgtcgatgat
     1681 gcgcctgccg gatatcgtgg gcgtcaagct caccggccag cgcaagcccg gcatcaccgc
     1741 caccgacatc gtgctggcgc tgaccgagtt tctgcgcaaa gagcgcgtgg ttggcgccta
     1801 tgtggaattc tttggcgaag gtgccgacag cctgtcgatt ggcgaccgcg ccaccatctc
     1861 caatatgtgc ccggaatacg gcgccacggc ggcgatgttc tacatcgacc agcaaaccat
     1921 cgactacctg aagctcaccg gccgcgagcc ggagcaggtg gcgctggtgg aaaactacgc
     1981 caagctgacc ggcctgtggg ctgagtcgct gaaaaccgcg caatacgagc gcgtgctgga
     2041 gtttgatctg tccagcgtgg tgcgcaatat ggctggcccg tccaacccgc acaagcgcct
     2101 gccgacttcc gccctgcaag agcgcggcat tgccgacgag gccaagctgg ccgccgcccg
     2161 cgccgaagaa gcccagggcc tgctgccgga tggcgcggtg gtgattgccg ccatcaccag
     2221 ctgcaccaac acctccaacc cgcgcaatgt ggtggccgcc gcgctgctgg ccaaaaaagc
     2281 caacgcgctg ggcctggtgc gcaagccgtg ggtgaagacc tcgtttgcgc cgggctccaa
     2341 ggtcgccaag ctgtacctgg aagaagccgg cctgctgccc gagctggaaa aactcggctt
     2401 tggcatcgtc gcctacgcct gcaccacctg taacggcatg tccggcgcgc tggacccgaa
     2461 aatccagcag gaaatcatcg accgcgacct gtacgccacc gccgtgctgt cgggcaaccg
     2521 caactttgac ggccgcatcc acccctacgc caagcaggcc ttcctggctt cgccgccgct
     2581 ggtggtcgcc tacgccatcg ccggcaccgt gcgctttgac atcgagcaag acgtgctggc
     2641 cgtggtggat ggcaaggaaa tccgcctgaa agacctgtgg ccgtcggatg aagaaatcga
     2701 cgccatcgtc gccgccgccg tcaagcccga gcagttcaag cagatctaca tcccgatgtt
     2761 cgatctgggc gaagccgagc aagccgaaag cccgctgtac aactggcgtc cgatgtccac
     2821 ctacatccgc cgcccgccgt actgggacac cgaaggcgtg ggcgcgctgg ccgccaatcc
     2881 gcgcacgctc aagggcatgc gcccgctggc cgtgctgccg gacaacatca ccaccgatca
     2941 cctgtcgccg tccaacgcca ttctggccag ctcggctgcg ggtgaatacc tggccaagat
     3001 gggcttgccg gaagaagact tcaactctta cgccacccac cgtggcgacc acctgaccgc
     3061 catgcgcgcc accttcgcca acccgcagct ggtgaacgaa atggccgtgg tggatggcca
     3121 ggtgaaaaag ggttcgctgg cccgcgtcga gccggacggc aaggtgctgc gcatgtggga
     3181 agccatcgaa acctatatgg accgcaagca gccgctgatc atcgttgccg gcgctgacta
     3241 cggccaaggc tccagccgcg actgggcggc caagggcgtg cgtctggcag gggtggaagt
     3301 gattgtcgcc gaag
//
LOCUS       DAOPYX010000322         3270 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_39791_length_3270_cov_3.842924, whole genome shotgun sequence.
ACCESSION   DAOPYX010000322 DAOPYX010000000
VERSION     DAOPYX010000322.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 3270)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3270)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3270
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39791_length_3270_cov_3.842924"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(232..1239)
                     /locus_tag="PKN55_12805"
     CDS             complement(232..1239)
                     /locus_tag="PKN55_12805"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNX72171.1"
                     /translation="MNIITLEHQDARINIEEIDAVSRRIRVEMKNSAMFSPRKQWVTR
                     YPVSLIEAVLQHRGPAYLCDEIARDEDPSYTASDIMASVFGYVSKSAMDGKRLLDFGC
                     GSGASTMIMARALPKCQIYGVELEPKPLALARLRAAHYRCDNLTLLQSPSPDALPPEI
                     QQVDYILLSAVYEHLLPVERPRLLRQMWELLAPGGVLFINQTPDDRFPLETHTTSLPF
                     INYLPDSLAGAFARTCSKRSLKNASWELMLRKGIRGASPGEIMGILSSFSSGSPQLLQ
                     PQAPGLKQQSDIWYHSARERLSQRYHGAKRLMVLSLIDAMIVSRLPVAPYLSLAIQKQ
                     A"
     gene            complement(1269..2048)
                     /locus_tag="PKN55_12810"
     CDS             complement(1269..2048)
                     /locus_tag="PKN55_12810"
                     /inference="COORDINATES: protein motif:HMM:NF012760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyl transferase"
                     /protein_id="HNX72172.1"
                     /translation="MAERHPFKVVMLCRDDDTARIMYHGLVPDWDIVGVIIEQKPSTR
                     QMLQRRMRKLGIWTVLGQVLFMLYTRLVGVRLAKAAREALLSAEQVTVAAFPSDKVRH
                     VASANDPETIRLLQAWNPDAVVVNGTRILSPSVLSSVSGPFINTHAGITPRYRGVHGG
                     YWALARHDAAHCGVTVHLVDEGIDTGSVLYQAVIEVGPDDSFVTYPLRQVIKAAPLMS
                     QALSDVKHGRLRPQVGVGPSGLWSHPTLWEYLANWLRSGVK"
     gene            complement(2041..3033)
                     /locus_tag="PKN55_12815"
     CDS             complement(2041..3033)
                     /locus_tag="PKN55_12815"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HNX72173.1"
                     /translation="MAQLNYGVFTISLDFELYWGVRDLCQIADYQQNLLGVRQAAPAM
                     LKAFSSAGVHATWATVGLLFCQDRVDALNQAPDLKPAYRRTELSPYHYLSEHALLDPI
                     YHFAPDLVAAIADTAGQELASHTFSHYYCLEAGQTAEAFRADLQAAQAVASRMGHHLR
                     SLVFPRNQWNAQYLSVLAEQGFRCFRGNESAWMYQAGATETAQHHPLRRALRLLDSYL
                     NLSGHHTYALTDCQDGAVCNFPASRFLRPYSPRLAALDGLRLQRITRAMTYAARHQQI
                     FHLWWHPHNFGRYLTENMAMLEKIISHFHVLEQQYGMRSLNMGELAALVEAPHG"
BASE COUNT          838 a          926 c          861 g          645 t
ORIGIN      
        1 tcgccggcca cgtatctggg caaggcagcg gagctggcca agcgcatctg agcgctgcac
       61 accccctcct ggggttgtgc cgactttgcc gtcctgtgtt tgcacgcgcc agccaggatt
      121 gctttgcgaa gtgttgtgag cgactcgcaa cctgagcgct tctcaacaga acaaagccaa
      181 aacgcctcgc agcgacacac tgcgaggcgt tttttatgca tgcggacagg gtcaagcctg
      241 tttctgaatg gccaaagaga ggtaaggcgc aacgggcaag cgcgacacaa tcatggcgtc
      301 aatcaggctc agaaccatca agcgcttggc gccatgataa cgctgcgata aacgctcacg
      361 agcagagtga taccaaatat cgctttgttg cttgagacca ggcgcctggg gttgcaataa
      421 ttgtgggctg ccgctagaaa agctcgacaa gatccccata atttcccccg gcgaagcgcc
      481 gcgaatgcct ttacgcaaca tcaactccca cgaggcattt ttcagactgc gtttcgagca
      541 tgttctggca aaagcgccag ccaggctgtc tggcaaataa ttgataaatg gcaggctggt
      601 ggtgtgggtt tccagcggaa aacgatcatc tggcgtttgg ttgatgaaca gcacgccgcc
      661 aggcgccagc aactcccaca tttgtcgaag caggcgcggg cgttcgactg gcaggagatg
      721 ctcgtacaca gcggataata aaatataatc cacctgctga atttcaggcg gcaaggcatc
      781 cggtgacggc gattgcaata aggtcaggtt atcacaccga taatgcgccg cgcgcaaacg
      841 cgccaaggcc aaaggcttag gctcaagctc aacgccataa atctggcatt taggcagggc
      901 gcgcgccatg atcatggtgg atgcgccaga accacagcca aaatccaaca ggcgctttcc
      961 gtccatggcg gacttgctga catagccaaa caccgaagcc atgatatcgc tggcggtata
     1021 agaagggtct tcatcccgag cgatttcatc gcataaatag gcaggcccac ggtgctgaag
     1081 cacagcctca atcaaactga caggatagcg cgtcacccac tgctttcgag gagaaaacat
     1141 ggcggagttt ttcatttcca ctcgaatgcg gcgagaaacg gcatcgatct cctcaatatt
     1201 gatccgtgca tcctgatgtt ccagtgtgat gatattcata caatgatttt gcctgacaga
     1261 gataaaattt atttgacgcc agaacgcagc caattcgcca gatattccca tagtgtggga
     1321 tgagaccata gcccagatgg gcctacgcca acctgagggc gcaagcgtcc atgtttgaca
     1381 tcgctcaatg cttggctcat caacggcgcc gccttgatga cttggcgcaa ggggtaagtc
     1441 acaaacgaat cgtctggccc tacctcaatc actgcctgat acaacacact gccagtatca
     1501 atgccttcat ccaccagatg caccgtcacg ccacagtgtg cggcgtcgtg gcgggcgagc
     1561 gcccagtagc cgccatgcac tccccggtag cgtggcgtaa tgccagcatg ggtgttgatg
     1621 aatggcccag acaccgatga caacaccgag ggcgacaaaa tgcgcgttcc attaaccact
     1681 acggcatccg ggttccacgc ctgcaacagg cggattgttt cggggtcatt cgcagacgcc
     1741 acatgcctca ccttgtcaga tggaaaagcc gccaccgtca cttgctcggc tgatagcaaa
     1801 gcctctcgtg cagcttttgc caagcgcacg cccaccaatc gggtgtacaa catgaacagc
     1861 acttgcccaa gcacagtcca gatgccaagc ttgcgcatgc gtcgctgcaa catctgacga
     1921 gtggaaggct tttgttcgat gatcaccccc acgatgtccc agtccgggac aaggccgtgg
     1981 tacatgatgc gggcggtgtc atcgtcccgg cacaacatga ccactttgaa tggatggcgc
     2041 tcagccatgt ggagcctcca ccagcgccgc cagttcgccc atattcaacg agcgcatgcc
     2101 atactgctgc tcaagcacat gaaaatgcga aataattttc tctagcatag ccatattttc
     2161 tgtcaaataa cggccaaaat tatgaggatg ccaccagaga tgaaaaatct gctgatgccg
     2221 cgctgcatag gtcatggcgc gggtgatgcg ttgtaaacgc aaaccatcca gtgcggccag
     2281 ccggggagag tagggcctca aaaaacgact tgccggaaaa ttgcacaccg cgccatcctg
     2341 acagtccgtc aaggcgtagg tgtgatgacc agatagattc aggtagctgt ccagcaaacg
     2401 cagggcgcgt cgcaagggat gatgttgagc cgtctcggtt gctcccgctt gatacatcca
     2461 cgcagactca ttgccgcgaa aacaccgaaa cccttgttcc gccagcaccg acagatactg
     2521 cgcattccac tgattacggg gaaacaccaa gctgcgcaaa tggtggccca tgcggctggc
     2581 gacggcttgt gccgcttgca agtcagcccg gaaagcctct gcagtttgtc ctgcttcaag
     2641 gcagtagtaa tgggaaaacg tatgcgatgc caactcttgt cccgctgtgt cggcaatcgc
     2701 cgcaaccaga tcaggcgcaa aatggtaaat cgggtccagc agggcatgct cagaaaggta
     2761 gtggtacggc gacaattctg tgcgccgata tgcgggttta agatcgggcg cctggtttag
     2821 cgcatccaca cgatcctggc aaaacaatag gcctacagtc gcccaagtgg catgcacgcc
     2881 agcagaagaa aacgccttaa gcatagccgg cgcagcctgg cgcaccccaa ggagattttg
     2941 ctgataatca gcaatttggc ataagtctcg caccccccag tacagctcaa aatccaggga
     3001 aatggtgaat accccataat taagctgggc cacgaaacac ctcaacatca ctcaaaaaat
     3061 agtcgaaaaa tattcacgct cgtctgttca tgcccagtcg ctcctgccgt cgctccccag
     3121 aaaaatttac tcggcgcggt ggcgcaaaat aagccattta ggcgtcttcc agtgcgccag
     3181 gcaatggtat agccaagcgt acaccaaggc gaacatgaag cagcacgaca acaacagtac
     3241 ggggtaacgc cagaacaata cggcagggat
//
LOCUS       DAOPYX010000323         3253 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_40093_length_3253_cov_8.811445, whole genome shotgun sequence.
ACCESSION   DAOPYX010000323 DAOPYX010000000
VERSION     DAOPYX010000323.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 3253)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3253)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3253
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40093_length_3253_cov_8.811445"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..428
                     /locus_tag="PKN55_12820"
     CDS             <1..428
                     /locus_tag="PKN55_12820"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNX72174.1"
                     /translation="GRGFAVVADEVRKLAERTQQATKDIDHKVTLIVSGTNHALVAMR
                     DGNEKMTHGASHARSAQDSLAAIIGGTRNLSRVLAKVSESGRSQTEGFREFTRDIEEI
                     GQSTQSLSAETEIIAGATRRLDKLMADLGDTVRTLEAGR"
     gene            573..1511
                     /locus_tag="PKN55_12825"
     CDS             573..1511
                     /locus_tag="PKN55_12825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012696961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complex I NDUFA9 subunit family protein"
                     /protein_id="HNX72175.1"
                     /translation="MSSGPQHIALIGGTGFVGTHLIEWLVRRQRQVRVLTRRRERCKA
                     LATLPGVSLHEGGAESLDALLAGQDAAVYLVGILHGSRQDFERAHVGLLQDVIAACQR
                     QGVRRLVLVGALGAAVDAASDYQQTKAQGEAALMASGLDWTIVRPSVIFGRGDSFLNL
                     FADLLAVAPCLPLAGAGTRFQPVWVEDVAHVLADCLDRADTHGQRYELAGPETFTLAE
                     LVRYVGQLTGHPRPVFGLPEGLAMAQAALMERLPKPPMSRDNVRSLRHDNVSAQGFPS
                     ATFGFAPAALTSIAPGWLAGNTPRARYDSFRRLARR"
     gene            1522..2748
                     /locus_tag="PKN55_12830"
     CDS             1522..2748
                     /locus_tag="PKN55_12830"
                     /EC_number="2.7.7.72"
                     /EC_number="3.1.3.-"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018152934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004810 - CCA tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0001680 - tRNA 3'-terminal CCA addition
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional CCA addition/repair protein"
                     /protein_id="HNX72176.1"
                     /translation="MNFTCYMVGGAVRDRLLGRPAPDRDWVVVGATPEAMLAAGFKPV
                     GRDFPVFLHPRTHEEYALARTERKTAKGYHGFVCHAAPDVTLEEDLARRDLTINAMAQ
                     TADGDIIDPYHGQDDLRAGLLRHVSPAFAEDPVRILRLARFAARYGFAVAAETRALMQ
                     QMVADGEVDALVAERVWQELAKGLMETTPSAMFTVLRDCGALARIAPEINALWGVPQR
                     ADYHPEIDCGVHTLMVLDYAAQENQPLPVRFAALTHDLGKALTPADVLPRHLMHEQRG
                     LAPLKALCERLRAPVECRELALLMCAEHTLVHRAHELRADTVLKLFKRADALRRPARF
                     AQLLAACRADARGRLGFADSPYPQADYLAALLTAAQSVDAGAIARQCSNPADIAKAVD
                     AAREAAVAGEKARLGK"
     gene            complement(2951..>3253)
                     /locus_tag="PKN55_12835"
     CDS             complement(2951..>3253)
                     /locus_tag="PKN55_12835"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018150825.1"
                     /note="catalyzes the formation of malate from glyoxylate
                     and acetyl-CoA; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HNX72177.1"
                     /translation="DINDIGLMEDRATLRISSQHVANWLHHGVVTEAQVRESFARMAA
                     KVDGQNAGDPAYQSLVANPDGAAYQAALELVFKGKEQPSGYTEPLLHAWRLKVKAA"
BASE COUNT          539 a         1134 c         1060 g          520 t
ORIGIN      
        1 ccgggcgcgg ctttgccgtg gtggccgacg aagtgcgcaa gctggccgaa cgcacccagc
       61 aagccaccaa ggacatcgac cacaaagtca ccctgattgt cagcggcacc aaccacgccc
      121 tggtggcgat gcgcgacggc aacgaaaaaa tgacccacgg cgccagccac gcccgcagcg
      181 cgcaggatag cctggcggcg attatcggcg gcacacgcaa tctgtcgcgc gtgctggcca
      241 aagtgtcaga atccggccgc tcgcaaaccg aaggattccg cgagttcacc cgcgacatcg
      301 aagaaatcgg ccaatccacg caaagcctgt cggccgaaac cgaaatcatc gccggcgcca
      361 cccgccggct ggacaagctg atggcggatt tgggcgacac cgtgcgcacg ctggaggcgg
      421 ggcgctgagc gcgctgtttc actactgacc ataccggcgc catgcaagca cacccggctg
      481 gttttgtgcg cagcgctcgg ccgattggct gtgacagtca caattccttg ctatcatgaa
      541 gctctcgtta cgcggttttt ttggagtgct ttatgtcttc tggtccgcaa cacatcgccc
      601 tgattggcgg cacaggtttt gtcggcacgc atttgatcga atggctggtg cgccgtcagc
      661 gccaggtgcg cgtgctcacc cgtcggcgcg agcgctgcaa ggcgctggcc accttgccgg
      721 gcgtcagcct gcacgaaggc ggcgccgaat cgctggatgc gctcctggcc gggcaggacg
      781 cggcggtgta tctggtgggg attttgcacg gcagccggca agactttgag cgcgcccatg
      841 tcgggctgct gcaagacgtg atcgccgcct gccagcgcca gggcgtgcgc cggctggtgc
      901 tggtgggcgc gctgggcgcg gcggtggacg ccgcctcgga ttatcagcaa accaaggccc
      961 agggcgaagc cgcgctgatg gccagcgggc tggattggac cattgttcgc ccctcggtga
     1021 tttttggccg gggcgacagc tttttgaacc tgtttgccga cctgctggcc gtggccccct
     1081 gcctgccgct ggccggcgcc ggcacgcgct ttcagccggt gtgggtggaa gacgtcgccc
     1141 atgtgctggc cgactgcctg gaccgcgccg acacccacgg ccagcgttat gagctggccg
     1201 gcccggaaac cttcaccctg gccgagctgg tgcgctatgt cggccagctc accggccacc
     1261 cgcgcccggt gtttggcctg ccggaagggc tggccatggc gcaggcggcg ctgatggaaa
     1321 ggctgcccaa gccgccgatg tcgcgcgata atgtgcgctc gctgcgccac gacaatgtca
     1381 gcgcgcaggg ctttccctcg gcaacctttg gctttgcccc cgccgcgctg accagcattg
     1441 cgccgggctg gctggccggc aacaccccgc gcgcgcgtta cgacagcttc cgccgtctgg
     1501 cgcggcgctg aaacggcagg catgaacttt acgtgttata tggtaggcgg cgccgtgcgc
     1561 gaccgcctgt tgggccgccc ggccccggat cgggactggg tggtggtggg cgccacgccc
     1621 gaggccatgc tggccgccgg cttcaagccg gtggggcggg attttccggt gtttttgcac
     1681 ccgcgcaccc acgaggaata cgcgctggcg cgcaccgagc gcaaaaccgc caagggctac
     1741 catggctttg tctgccacgc cgcgcccgac gtcaccctgg aagaagacct cgcccgccgc
     1801 gacctgacca tcaacgccat ggcgcaaacc gccgacggcg acatcatcga cccctaccac
     1861 ggccaggacg acctgcgcgc cggcctgctg cgccacgtca gccccgcctt tgccgaagac
     1921 ccggtgcgca tcttgcgcct ggcgcgcttt gccgcccgct acggctttgc cgtggcggcg
     1981 gaaacccgcg cgctgatgca acagatggtc gccgacggcg aggtggacgc gctggtggcc
     2041 gagcgggtgt ggcaagaact ggccaagggc ctgatggaaa ccacgccctc agccatgttc
     2101 accgtgctgc gcgactgcgg cgcgctggcg cgcatcgccc cggaaatcaa cgcgctgtgg
     2161 ggcgtgccgc aacgcgccga ctaccacccg gaaatcgact gcggcgtgca caccctgatg
     2221 gtgctggact acgccgcaca agaaaaccag ccgctgcccg tgcgctttgc cgccctcacc
     2281 cacgacctgg gcaaggcgct cacccccgcc gacgtgctgc cgcgccacct gatgcacgaa
     2341 caacgcggcc tcgccccact caaagccctg tgcgaacgcc tgcgcgcgcc agtggaatgc
     2401 cgcgaactgg ccctgctgat gtgcgccgaa cacaccctgg tgcaccgcgc ccacgaactg
     2461 cgcgccgaca ccgtgctgaa actgttcaaa cgcgccgacg ccctgcgccg ccctgcgcgc
     2521 tttgcccaac tgctggccgc ctgccgcgcc gacgcccgtg gccgcctggg ctttgccgac
     2581 agcccctacc cacaagccga ctacctggcc gccctgctta cagccgcgca aagcgtggac
     2641 gccggcgcca tcgcccgcca atgcagcaat ccggcggata ttgccaaggc ggtggacgcg
     2701 gcgcgggaag cggcggtggc gggggagaaa gcacgattgg ggaaatgaac gcgcgccaca
     2761 ggccataagc gccgctcaca acacccagca cagcgatcct ggtcaggcac acgaccgcag
     2821 acaacggcaa taaggtcagg aaggcttgga gagctgttct aaaaacaaaa agcgccgctt
     2881 cggcgctttt tgtttggctt caccgccgca gttcaaaccc acggcggccg ctcagtcagg
     2941 cgtggcaagc tcaagccgcc ttcaccttca accgccaggc atgcagcagc ggctcggtgt
     3001 agccgctcgg ctgttccttg cccttgaaca ccagttccag cgcggcttgg taggcggcgc
     3061 cgtccgggtt ggccaccagg ctttggtacg ccggatcgcc agcgttctgg ccgtccacct
     3121 tggcggccat gcgggcgaag ctctcgcgca cttgcgcttc ggtcaccacg ccgtggtgca
     3181 gccagttggc gacgtgctgg ctggagatgc gcagggtggc gcggtcttcc atcaggccga
     3241 tgtcgttgat gtc
//
LOCUS       DAOPYX010000324         3248 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_40213_length_3248_cov_3.384278, whole genome shotgun sequence.
ACCESSION   DAOPYX010000324 DAOPYX010000000
VERSION     DAOPYX010000324.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 3248)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3248)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3248
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40213_length_3248_cov_3.384278"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1315)
                     /locus_tag="PKN55_12840"
     CDS             complement(<1..1315)
                     /locus_tag="PKN55_12840"
                     /inference="COORDINATES: protein motif:HMM:NF012573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HNX72178.1"
                     /translation="MTSISSLTSYTLAPLDDQLYLLGTANINGTGNIKANAIFGNIAD
                     NILSGGQGSDSLYGGDGNDTLYASAPGFVDATSQDWLYGGAGHDKLYADGGDRLYGEA
                     GDDSLYTGNASNNQLYGGVGNDVYMLNLYANQVIEQAGEGIDTVYSSVSLALDNSIAS
                     TSLISGEVENLILANGVAQGGGNALNNLIIGQTGNNTLWGGAGQDTLQGGDQDDVLYG
                     NAASVLADPTQDRLEGGAGNDTLYGDGNDILLGGEGADTYIVRSADNTIIETTTAAID
                     TVKSHVSLSLGLVWAGVPASLVSGAVERLELQGSGNLIGLGQDGLNNTLLGNTGDNLL
                     MGGSGQDTLYGGSGQDVLHGNILSGGINAEVDWLYGEAGNDTLYGDGGDWLDGGAGDD
                     VYYILGAGNHVRETSSGGVDTVRSYVTFGIGGMGGAGLDCSPWIEN"
     gene            complement(1785..>3248)
                     /locus_tag="PKN55_12845"
     CDS             complement(1785..>3248)
                     /locus_tag="PKN55_12845"
                     /inference="COORDINATES: protein motif:HMM:NF012573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HNX72179.1"
                     /translation="MVSVALDNSLAGAPLISGEVENLTLTNGPVNLKGGGNALNNLIT
                     GNVSANILWGSVGADTLQGGGGNDVLYGNSATVLADAAQDWLYGGDGDDTLYADGNDM
                     LLGGVGADTYIIRAGLANTIIEDLTPSAVVDTVKSEISFSLGLSVPVAGMPTTQVASG
                     AVERLELQGSADLIGLGSNGVNNTLQGNSGNNVLMGGTGMDILYGGAGNDTLYGNVVN
                     GATNTEQDMLYGEIGNDTLYGDGYDVLYGGVGDDIYYINGINNHVQEMTGEGFDTVRR
                     YVSFGVGGSPDPQSSNSSPGIEVIELVGTAHISAYGSGDAETLLGNSGNNVLNGRGGH
                     DLLNGGAGNDTLKVTVALDPAVYGDQNVTLIGGAGTDIFAIGASGKLVGANNTVLISD
                     FQHGVDKIMFNTIAGPGTLLTLTTPAGATLDDMLTLATSQMTTSNTISQFVFAGDTYL
                     VMDRSSSNGFASADTAIKVAGVPLITWSDLAFGVVGV"
BASE COUNT          675 a         1008 c          863 g          702 t
ORIGIN      
        1 cattttcaat ccacgggctg caatccaggc cggcgccccc catgccgccg atgccaaagg
       61 tgacatagct gcgcacggtg tccacaccgc cgctggatgt ttcgcgcaca tggttgccgg
      121 cacccaggat gtaatacaca tcgtcgccag cgccaccgtc cagccagtcg ccgccatcgc
      181 catacaaggt gtcgttgccg gcttcgccat acagccaatc cacctcggcg ttgatgccgc
      241 cactgaggat attgccgtgc agcacatcct gcccgctgcc gccatacagc gtgtcttggc
      301 cggagccgcc catcagcaga ttgtcgccag tattgcccag caaggtattg ttgaggccat
      361 cctgccccag cccaatcaga ttgcccgagc cttgcagctc caggcgctca acagcgccgc
      421 tgaccaagct ggctggcacg cccgcccaga ccaggcccag cgataaagac acatggcttt
      481 tgactgtatc gatggcggca gtggtggttt cgatgatggt gttgtcggca ctgcgcacaa
      541 tataagtatc cgctccctca ccgcccagca ggatatcgtt gccgtcacca tacagggtgt
      601 cgttgccggc gccgccttcc agcctatcct gggtggggtc cgccagcacg ctggcggcgt
      661 tgccatacaa aacatcatct tgatcgccac cttgcaaggt atcctggccg gcgccgcccc
      721 acagcgtatt gttaccggtt tggccaataa tcagattgtt cagggcattg ccgccgcctt
      781 gcgccacccc attggccagg atgaggtttt cgacttcgcc gctgatcagc gacgtgctgg
      841 caatcgaatt atccagcgcc agcgagacgc tgctgtacac cgtgtctatg ccttcccctg
      901 cttgctcaat cacttgattg gcatacagat tgagcatgta cacatcattg cccacgccgc
      961 catacagctg attattgctg gcattgccgg tatacaagct atcgtcaccc gcctcgccat
     1021 acagccgatc gccgccatcg gcgtacagtt tgtcgtggcc tgcgccgcca tacagccagt
     1081 cttgggaggt ggcgtccaca aagcccggcg ctgaggcata cagcgtatcg ttgccgtcac
     1141 caccatacaa actgtcgctg ccttgccccc cgctgagtat attgtctgcg atattgccaa
     1201 agatagcatt ggccttgatg ttgcccgtgc cattgatatt ggccgtgccc agcaaataaa
     1261 gctggtcatc caggggggcc aaggtgtagc tggtcaggct gctgatgctg gtcatgattt
     1321 tattctccag gacgtgaaag cggagattcc gccatttcaa tgtacgcgcc tgtttgccta
     1381 agtaacaatc gtataactac ttagtggaat aaaaatatgt attgtgtctt agtggtttga
     1441 gtgtttttta cgcaggtcga cctgaaaata taagagtgat ttatttggtg tggcgtcaca
     1501 aaaaagccga aaagcatgca atgaattggc gggcagagaa actcttttgt tatcaataag
     1561 atgaatgggg tgtgttgata aaaaataaaa ctggccagat ttccatgtgg cgtatctggc
     1621 cagttgcggg tgttgccgaa aaacgacgcg tgctttgccg tgtgcgcctt accgctgagc
     1681 gcggctcacc aaacccaaac acgccatcct ggccaggctg ggcagacagc acaacaaggc
     1741 caggcgggtt gtgtcaacag cacatcacac actcggcaaa gctgttacac ccccactaca
     1801 ccaaacgcca aatcagacca ggtaatcagc ggcacgccag ccaccttgat ggcggtatct
     1861 gcgctggcaa aaccattgct ggaggagcga tccatcacca gataggtgtc accggcaaac
     1921 acaaactggc tgatggtgtt gctcgtggtc atctggctgg tagccagagt gagcatatcg
     1981 tccagcgtgg cgccagccgg cgtggtcaag gtcagtaatg tgcctgggcc ggcgatggtg
     2041 ttaaacatga tcttgtccac gccatgctgg aaatcactga tcaacaccgt gttattggcg
     2101 cccaccagct tgccgctggc gccaatggcg aagatatccg tgcccgcgcc gccaatcagg
     2161 gtgacgttct gatcaccata cacagcggga tccagcgcta ccgttacttt cagcgtgtcg
     2221 ttgcctgcgc cgccattgag cagatcgtga ccgccacggc cattgagcac attattgcca
     2281 ctgttgccca gcagggtttc cgcatccccg ctgccatagg cgctgatatg cgccgtgccc
     2341 accagctcga tcacttcaat gcccgggctg ctgttggagc tttgcggatc gggcgagccg
     2401 cccacgccaa acgacacata cctgcgcacg gtgtcaaaac cttcaccggt catttcctgc
     2461 acatggttgt tgatgccgtt gatatagtaa atatcgtcgc ctaccccgcc atacagcaca
     2521 tcgtagccat cgccatacag ggtgtcgttg ccgatttcgc catacagcat atcctgctcg
     2581 gtattggtcg cgccattcac cacattgcca tacagggtat cgttgccagc gccgccatac
     2641 aggatatcca tgccggtgcc gcccatcagc acattattgc cgctgttgcc ctgcagggtg
     2701 ttattcaccc cattgctgcc cagaccgatc aaatcagccg agccttgcag ctccagccgc
     2761 tccactgcgc cagacgccac ttgggtggtc ggcatgccgg ccactggcac gctcaggccc
     2821 agcgagaacg aaatctcgct cttgaccgta tccaccactg ccgatggggt gaggtcttca
     2881 ataatggtgt tggccagccc agcgcggatg atgtaggtgt cagcgccgac gccgcccagc
     2941 agcatatcgt tgccgtcagc gtacagggtg tcgtcgccat cgccgccgta cagccaatcc
     3001 tgcgccgcgt cggccagcac cgtggcggaa ttgccataca gcacatcgtt gccgccgccg
     3061 ccttgcaggg tgtcggcgcc cacgctcccc cacaggatat tggcggagac attgccggta
     3121 atcagattat tcagcgcatt gccgccgcct ttcagattca ccgggccatt ggtcagggtg
     3181 aggttttcca cctcgccact gatcagcggc gcgccagcaa gggagttatc cagcgccacc
     3241 gacaccat
//
LOCUS       DAOPYX010000325         3248 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_40220_length_3248_cov_2.808018, whole genome shotgun sequence.
ACCESSION   DAOPYX010000325 DAOPYX010000000
VERSION     DAOPYX010000325.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 3248)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3248)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3248
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40220_length_3248_cov_2.808018"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..154)
                     /locus_tag="PKN55_12850"
     CDS             complement(<1..154)
                     /locus_tag="PKN55_12850"
                     /inference="COORDINATES: protein motif:HMM:NF012674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body protein"
                     /protein_id="HNX72180.1"
                     /translation="MGFQQGLSGVSAAAKQLDVIGNNVANSSTVGFKQSRAEFGDMFA
                     ASYYGVA"
     gene            complement(237..911)
                     /locus_tag="PKN55_12855"
     CDS             complement(237..911)
                     /locus_tag="PKN55_12855"
                     /inference="COORDINATES: protein motif:HMM:NF025230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HNX72181.1"
                     /translation="MATTSSVTDSNPYNFLNGDTAATAKKSSIEEAQDRFMKLLVTQL
                     QTQDPMNPMDNAAVTSQMAQISTVQGIEKLNQSMTSLNEMYKSSQSVAAAALIGHIAL
                     ASGSQMVLSSGKAVAGVDLAKNADKVTINVNNADGKTVYTEVLNDQKAGVLQFQWDGK
                     DDSGNQLADGNYSLSVTASQGDTSVEATPLAYSTVQAMSWNNGTPMLHLANGKETTVD
                     AIRQLI"
     gene            complement(933..1346)
                     /gene="flgC"
                     /locus_tag="PKN55_12860"
     CDS             complement(933..1346)
                     /gene="flgC"
                     /locus_tag="PKN55_12860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008953790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HNX72182.1"
                     /translation="MSLSKVFDIAGAGMTAQSMRLNVVSSNIANVDSAVSSNGEPYKG
                     KKVVFTAIPINKETPQVATVRVTAVVEDQSPARMVYDPKHPMADAKGYVTMPNINPVE
                     EMTDMISSARSYQTNVEMMNTAKSLLQKTLSIGQQ"
     gene            complement(1354..1905)
                     /gene="flgB"
                     /locus_tag="PKN55_12865"
     CDS             complement(1354..1905)
                     /gene="flgB"
                     /locus_tag="PKN55_12865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012695933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009429 - bacterial-type flagellum basal
                     body, proximal rod [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HNX72183.1"
                     /translation="MAGAACAASRDDKDSKKLLVKQRTEKLLIATARLAQLLLIVMLP
                     TEFRTMLDKIAKHFDFQQKALGLRAYRTEVLSSNIANSDTPYYKAVDFDFKDAMTRAI
                     GESQSSMAMDKTHQTHLDGFAEQADKDFKLQYRNEVQPSIDGNTVDMDLERSAFADNA
                     LKYQSTLTFLNRRISGLSDAIKG"
     gene            complement(1987..3135)
                     /locus_tag="PKN55_12870"
     CDS             complement(1987..3135)
                     /locus_tag="PKN55_12870"
                     /inference="COORDINATES: protein motif:HMM:NF025214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX72184.1"
                     /translation="MIAAQAGPHAQLQRSLPISPLRIGFVSADLFRHSVSYFVEPVWD
                     GLRAQGMKVLAYYNGKQYDDISARLHRKCDIWREVADLSDDQLVAQIQADEVDVLIDL
                     SGHTGGNRLPVFARQPAPLQITWLGHPATTGIPAIQWRITDHHAEPEGLTEHLSSEQL
                     YRMPEVFCCYQPSAAPAPATKPPYDETGVFTLGCFNNFAKVTPPVIDCWGELLRQLPQ
                     ARLLLEIHGAEEDAFADDIRQRFVAAGARPEQVDILPRRAGQQYVLYRQVDLALDPFP
                     CCGGTTSCDSLWMGVPFVTLAGNSFVSRMGVTLLHNIGLAELIAQDVAGYQALVLALA
                     RDLPRLRALREGLRERVQTSPLMDGPRFARHFAQGLQQMWQAQIGRAQ"
BASE COUNT          693 a          895 c          885 g          775 t
ORIGIN      
        1 cagcgacacc atagtaactc gctgcaaaca tatcaccgaa ctcggcacgg gattgcttga
       61 aacccacggt gctggagttg gccacgttgt taccaatgac gtcaagctgc ttggctgctg
      121 ccgaaacgcc gctaagacct tgttgaaaac ccataatact ctccgttttg ttcagatggc
      181 gtgtgccaag ttcgtgtttc tgatatgacg cgtgcgctaa gactatgcgt atcgatttag
      241 atcagctggc gaatggcgtc aaccgtggtt tccttgccgt tggccagatg cagcatcggc
      301 gtgccattgt tccacgacat ggcctgaacg gtggagtaag ccagcggagt ggcttccacg
      361 ctggtgtcac cctgcgatgc tgtcacagac aggctgtagt tgccatcggc cagctggttg
      421 ccggagtcat ccttgccatc ccactggaat tgcagcacgc ccgctttctg gtcgttcagc
      481 acttcggtgt acaccgtttt gccgtcggca ttattgacgt taatagtaac cttatcggca
      541 ttttttgcca aatccacccc ggcaactgcc ttaccgctgg acaacaccat ctggctgcca
      601 ctggccagag caatatggcc aatcagggcg gcagcggcaa ccgattggct gctcttgtac
      661 atttcattca gagaagtcat cgattgatta agcttttcga tgccttgcac cgtgctgatc
      721 tgggccatct ggctggtcac ggctgcgtta tccatcggat tcatcggatc ctgggtttgc
      781 aactgggtca ccagcagctt cataaagcga tcttgggctt cttcaatgct gcttttcttc
      841 gccgtggcgg cggtgtcgcc attcagaaag ttgtagggat tgctgtcggt aacggaagac
      901 gtggtagcca tgatgttctt ttaacctgtg agttattgct gaccaatgga cagcgttttc
      961 tgcaacagtg acttggccgt attcatcatt tccacattgg tctggtaaga gcgggccgac
     1021 gagatcatgt cggtcatttc ttccaccggg ttgatgttgg gcatggtcac atagcctttg
     1081 gcgtcagcca tcggatgttt cgggtcgtag accatccggg ccggcgactg atcttccacc
     1141 acggcggtca cccgcacagt ggccacttgc ggcgtttcct tgttgattgg aatcgccgtg
     1201 aacaccacct ttttgccctt gtacggttcc ccgttggagg acactgcgct gtcaacgttg
     1261 gcaatattgc tggacaccac gttcaggcgc atggattgtg cggtcatgcc tgcgccagcg
     1321 atatcgaata ctttagataa agacatgatt caattacccc ttgatagcat cagacaggcc
     1381 ggaaatccgg cggttcagga aggtcagtgt ggactggtac ttcagcgcat tgtcagcaaa
     1441 ggctgagcgt tccaggtcca tgtcgacggt gttgccgtca atcgacggct gaacttcatt
     1501 gcggtattgc agcttgaagt ccttgtctgc ctgctcggca aagccatcca gatgggtttg
     1561 gtgggttttg tccatcgcca tggaggactg gctctcgcca atggcgcggg tcatggcgtc
     1621 cttgaagtca aaatcgactg ccttgtagta aggcgtgtca gagttggcga tgttactcga
     1681 cagcacttcc gtgcggtatg cacgcaagcc cagcgctttt tgctgaaagt cgaaatgttt
     1741 ggcaatttta tcgagcatgg ttctaaactc cgtcggcaac atcacgatta gcaataactg
     1801 cgccagcctg gctgttgcga tcaatagttt ttcagtgcgt tgtttaacaa ggagtttttt
     1861 tgaatccttg tcatctctgc tggcggcgca cgcagcgcca gccattcacc cggcaagttt
     1921 tgccggtctc ggcaaaactt gcaactaaaa aaatgggttc tgctgccgat agacagcaga
     1981 caatacttac tgcgcgcgcc cgatttgcgc ttgccacatc tgctgcaagc cttgggcaaa
     2041 gtgacgggca aaacgtggcc catccatcag ggggctggtt tgcacccgtt cgcgcaagcc
     2101 ttcgcgcagt gcgcgcaggc gtggcaggtc cctggccagc gccagcacca gcgcctgata
     2161 cccggcaaca tcctgtgcaa tcagctctgc cagtccgata ttgtgcagca gcgtcacgcc
     2221 catgcgagac acaaaactgt tgcctgccag cgtgacaaac ggcacaccca tccacaggct
     2281 gtcgcaactg gtggtgcccc cgcagcacgg gaaaggatcc agcgccagat caacctgacg
     2341 atacagcaca tactgctgcc ccgcccggcg cggcaggatg tccacttgct caggccgggc
     2401 gccagcagcc acaaagcgct ggcggatatc gtcggcaaat gcgtcttctt cggcgccgtg
     2461 aatttccagc agcaagcgcg cctgcggcaa ctgccgcaac agttcgcccc agcaatcgat
     2521 taccggtggg gtcaccttgg caaaattatt gaagcaaccc agggtgaata ccccggtttc
     2581 atcataggga ggcttggtgg cgggagccgg ggccgcactt ggctgatagc agcagaagac
     2641 ttcgggcatg cggtaaagct gctcgctgct cagatgctcg gtcaaaccct ctggctcggc
     2701 gtgatgatcg gtaatgcgcc actgaatggc cgggatgccg gttgttgccg ggtggcccag
     2761 ccaggtgatc tgcaacgggg cgggctgacg ggcaaaaacc ggtaagcggt tgccgccagt
     2821 atgcccggac aggtcaatca gtacgtccac ctcatccgcc tggatttgtg ccaccaattg
     2881 gtcatctgac aagtcggcca cttcgcgcca gatatcacat ttgcgatgca gacgggcgct
     2941 aatgtcatca tattgcttgc cattgtagta cgccagaacc ttcattcctt gtgcgcgcaa
     3001 gccatcccat accggctcga caaaataact cactgaatga cgaaacaaat cagcagacac
     3061 aaaaccaatc cgtaacggag aaataggtaa gctgcgttgc agctgggcat gcggaccagc
     3121 ttgggctgcg atcatttttc cgtaagcttt atgctcttct gccaactgaa cagctgaaat
     3181 agcatggctg taaatattag caaacagtaa gttgctgcga atatgccaat tatttggctg
     3241 catagcca
//
LOCUS       DAOPYX010000326         3223 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_40650_length_3223_cov_11.766414, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000326 DAOPYX010000000
VERSION     DAOPYX010000326.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 3223)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3223)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3223
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40650_length_3223_cov_11.766414"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..708
                     /locus_tag="PKN55_12875"
     CDS             <1..708
                     /locus_tag="PKN55_12875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HNX72185.1"
                     /translation="GIGFTLNNPVLIIAGACVGSSGAILSYIMCKAMNRSILSVLLGG
                     FGAEVAAGGGGDSGPKNYKSGSAEDAAFLMANADSVVIVPGYGLAVSRAQHALQEFAE
                     LLHEKGVNVRYAIHPVAGRMPGHMNVLLAEAEVPYDQVLEMEEINSDFSNTDVVLVIG
                     ANDVVNPAALKDKSSPIYGMPILEAHKARTIIVVKRSMSVGYAGLDNELFYMDKTMMV
                     FGDAKKVVEDLVKGVVH"
     gene            835..1425
                     /locus_tag="PKN55_12880"
     CDS             835..1425
                     /locus_tag="PKN55_12880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX72186.1"
                     /translation="MPNQDPISKQLPLKFANGQTIRRFAKHCPACKHIVDSQHMHGVA
                     QLVQDRVVIVAQARCPACGHGFSVTCVVTDDKQVHRVWVPGWALRWWLSRQRDDDAPL
                     RDRDSREWEYEDTPDVPTPLRNPTPSLSAADVTVASEALGQFQGEPIPAWIESQGQRY
                     VFQRASQDGPRTKLGRDEVLYEGRLVYVRDGVHVEV"
     gene            complement(1568..2560)
                     /locus_tag="PKN55_12885"
     CDS             complement(1568..2560)
                     /locus_tag="PKN55_12885"
                     /inference="COORDINATES: protein motif:HMM:NF019371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="HNX72187.1"
                     /translation="MSYAKPWKSYADQLNQLIDRGMVVTDRDRALDYLKRIGYYRLSG
                     YWFAFRERSAPLCLLDGQGRKPKKVREERIALDLFRAGTTFQNAVDLYVFDKQLRLLV
                     MDALERIEVALRVDVSHTLGQLDRFAYLKPELFHDEFSVKLDKDSGVTRHHEWLGKHA
                     QLIGRSKEEFVRHNRTKYGLPLAIWVACEVWDFGTLSTLFNGMRTTEQDAIASQYGIS
                     DGRVFATWLRSLNYLRNVCAHHSRLWNRNIVDQPRLPSSAELAWMTPFESDTHARARC
                     FLLLRITRHLLGVINPRSSWPGRLKAHLHAFPDLSHLGLNLKGMGVPDGWEADW"
     gene            complement(2843..>3223)
                     /locus_tag="PKN55_12890"
     CDS             complement(2843..>3223)
                     /locus_tag="PKN55_12890"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HNX72188.1"
                     /translation="LVMHGSSSVPQEWLAIINEFGGDLGETYGVPVEEIVEGIKHGVR
                     KVNIDTDLRLASTGAIRRFLAQNPKEFDPRKYLKVSTDAMRDICIARYEAFGACGQAA
                     KIKPVSLDAMANLYLKGQLAQIVK"
BASE COUNT          630 a          996 c          964 g          633 t
ORIGIN      
        1 ggcatcggct ttaccctgaa caacccggtg ctgatcattg ccggcgcgtg cgtgggttct
       61 tctggcgcga ttctgtctta catcatgtgt aaagccatga accgctccat cctcagcgtg
      121 ctgctgggcg gctttggcgc cgaagtggcg gcgggcggcg gcggcgacag cggcccgaag
      181 aactacaaat ccggttcggc cgaagacgcc gccttcctga tggccaacgc cgacagcgtg
      241 gtgatcgtgc cgggttacgg cctggccgtg tcgcgcgccc agcatgcgct gcaagagttt
      301 gccgagctgc tgcacgaaaa aggcgtgaac gtgcgctacg ccatccaccc ggtggcgggc
      361 cgcatgccgg gccatatgaa cgtgctgctg gccgaagccg aagtgccgta tgaccaagtg
      421 ctggaaatgg aagaaatcaa ctcggacttc tccaacaccg acgtggtgct ggtgatcggc
      481 gccaacgacg tggtcaaccc ggccgcgctg aaagataaaa gcagcccgat ttacggcatg
      541 ccgattctgg aagcgcacaa ggcgcgcacc attatcgtgg tcaagcgttc gatgagcgtg
      601 ggctacgccg gcctggacaa cgagctgttc tatatggata aaaccatgat ggtgtttggc
      661 gacgccaaga aagtggtgga agacctggtc aagggcgtgg tgcactgatt tttgccccgc
      721 ctcacccggc atcctgcccc gcccggccct ggcccggcgg ggtttttcat gcccaaggcc
      781 tgcgcgatgg gtgaaatttt gttactattt caacacgttc ccccagtatt cgccatgccc
      841 aaccaagacc ctatctccaa acagctgccc ctgaaattcg ccaacgggca aaccatccgc
      901 cgctttgcca agcactgccc cgcctgcaag cacattgtgg acagccagca tatgcacggc
      961 gtcgcccaac tggtgcaaga ccgcgtggtg attgtcgccc aggcgcgctg cccggcctgc
     1021 ggccacggct tttctgtcac ctgcgtggtc accgacgaca aacaagtgca tcgggtgtgg
     1081 gtgcctggct gggcgctgcg ctggtggctg tcgcgtcagc gtgacgacga cgcgccgctg
     1141 cgcgaccgcg acagccgcga atgggaatac gaagacaccc cggacgtgcc caccccgctg
     1201 cgcaacccca cccccagcct gagcgccgcc gacgtcaccg tcgccagcga ggcgctgggc
     1261 cagtttcagg gcgagcccat cccggcctgg atcgaaagcc agggccagcg ctatgtgttc
     1321 cagcgcgcca gccaggacgg cccgcgcacc aagctgggcc gcgacgaagt gctgtacgaa
     1381 ggccggctgg tgtatgtgcg ggatggggtg catgtggagg tgtaaaccgg gcgttgccac
     1441 ggtagcggct cgcgccgcca aaccttgacg gcgcataccc cgtcgcctag aatccatttc
     1501 aacacggccc ctctcggcaa ccccgcttcg gcgggaaagc tgctgagggg ttttttattg
     1561 cctgtgctca ccagtcggcc tcccagccat cgggcacgcc catgcctttc aggttcagtc
     1621 caaggtgaga cagatcggga aaggcatgca gatgtgcctt gagccggcca ggccaactcg
     1681 accgtggatt gatgacccct aacaaatgtc gggtgatgcg caagagcaag aaacagcgtg
     1741 cccgtgcgtg cgtatcgctc tcaaacggag tcatccaggc cagttcagca gatgacggca
     1801 agcgcggctg gtcaacgata ttccgattcc aaagacgact gtggtgtgcg cagacattgc
     1861 gcaggtagtt gaggctacgc agccatgtcg caaacacccg gccatcactg atgccgtatt
     1921 ggctggcaat cgcatcttgc tccgtcgtgc gcattccgtt gaacaacgtc gaaagcgtgc
     1981 cgaaatccca cacctcgcag gccacccaga tcgccagcgg taggccgtat ttcgtccggt
     2041 tgtgccgaac gaattcttct ttggagcggc cgatcaactg cgcgtgcttg cccagccact
     2101 cgtggtgccg ggtgacgccg gaatccttgt ccagcttgac gctgaactcg tcgtgaaaca
     2161 gctcgggctt gaggtaggca aagcggtcaa gctgacccaa agtgtgggac acatccacac
     2221 gcaacgccac ttcgatgcgt tccagggcat ccatcaccag cagccgaagc tgcttgtcga
     2281 acacgtatag atccaccgca ttttgaaagg tggtcccagc ccgaaagaga tcaagggcga
     2341 tgcgctcttc gcgcaccttc ttcggcttgc ggccctgtcc atccaacaga cacaaggggg
     2401 cgctgcgttc acggaacgca aaccagtagc cgctcaggcg gtagtagcct atgcgcttca
     2461 agtaatccag cgcacgatct cggtcggtga cgaccatgcc ccggtcgatc aattgattca
     2521 gttgatcagc gtagcttttc cagggtttgg catagctcat gagtcatatc cttcgcggtg
     2581 tacggtttaa tccacctcaa caatgcccat tctgacaaat aacccacaca cccaacaaaa
     2641 agcgcgacga aatccttcgt cgcgcttttt ttattgccgt agccagtcac catgattcag
     2701 aaccgactgg cgggtgctta gaccgctgta cgcggtgggc acgcctggat tcccgcctgc
     2761 gcgggaatga ctcggtgtaa tgcggcgaga ggctcttcgt ggcgcatgag ccttgtgctt
     2821 caacgtgact ggcggctagg gcttacttca cgatctgcgc cagttggccc ttcaggtaca
     2881 gattggccat ggcgtccagg ctcaccggct tgatcttggc ggcttggcca catgcgccaa
     2941 acgcttcgta gcgggcgatg cagatgtcgc gcatggcgtc ggtggagact ttcaggtatt
     3001 tgcgcgggtc gaattctttc gggttttgcg ccagaaagcg acggatggcg ccggtgctgg
     3061 ccaggcgcag gtcggtgtcg atgttcacct tgcgcacgcc gtgcttgatg ccttcgacga
     3121 tttcttccac cggcacgccg taggtttcgc ccaggtcgcc gccaaattca ttgatgatgg
     3181 ccagccattc ttgcggcacg ctggagctgc cgtgcatcac cag
//
LOCUS       DAOPYX010000327         3223 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_40652_length_3223_cov_9.473169, whole genome shotgun sequence.
ACCESSION   DAOPYX010000327 DAOPYX010000000
VERSION     DAOPYX010000327.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 3223)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3223)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3223
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40652_length_3223_cov_9.473169"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..907)
                     /locus_tag="PKN55_12895"
     CDS             complement(<1..907)
                     /locus_tag="PKN55_12895"
                     /inference="COORDINATES: protein motif:HMM:NF024949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4115 domain-containing protein"
                     /protein_id="HNX72189.1"
                     /translation="MSDIPTPGAESATHTASPGMLLSNERVKRGLSLQEVADRLKLSR
                     KQLEAIENDEYDKLPGPAFARGFVRNYAKLLGLDVALLNASLDAHLPMPPVNPPVADT
                     APAAPAPAASSRSPAVNPTLIKGALALLVLLLAAGVGYRLLTQGASTPATPPLAAPAV
                     DSALPQAALNPEPASSAAPVSAAPSVDSKPAELKPEPAKLEALAVAPVKPAEVKPAEA
                     KPTSTDTSTAQAAVSDTGAGELVLNAREKAWVSVIDAKGKKLLYGQISGEKRLGGGTP
                     PYKLIIGNAPQVELNYKGQAVPLADK"
     gene            complement(982..2070)
                     /gene="rlmN"
                     /locus_tag="PKN55_12900"
     CDS             complement(982..2070)
                     /gene="rlmN"
                     /locus_tag="PKN55_12900"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012696168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HNX72190.1"
                     /translation="MKTNLLDFTLPQLTDHFAAMGEKPFRATQVMRWMHQMGTDDFSA
                     MTDLAKTLRAKLEACAEVRVPQLMTGQASTDGTRKWLLDVGTGNGVETVFIPEDDRGT
                     LCVSSQVGCALECTFCSTGRQGFNRNLSTAEIIGQLWWANKSMGVTPKNERVISNVVM
                     MGMGEPLANYDNVVAAMQIMLDDHGYGLSRRRVTLSTSGLVPALDRLREDCPVALAVS
                     LHAPNDALRDQIVPINKKYPLRELLAACERYLQRAPRDFVTFEYVMLDGVNDTPEHAR
                     QLLALVKAVPCKFNLIPFNPFPNSGYARSSAGAIRTFREILQEAGYVVTVRKTRGDDI
                     DAACGQLAGKVEDKTRRAVRWLSDAAPE"
     gene            complement(2120..2545)
                     /gene="ndk"
                     /locus_tag="PKN55_12905"
     CDS             complement(2120..2545)
                     /gene="ndk"
                     /locus_tag="PKN55_12905"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006165 - nucleoside diphosphate
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HNX72191.1"
                     /translation="MAIERTLSIVKPDAVAKNVIGQIYSRFEAAGLKVVAAKMKHLSR
                     AEAEGFYAVHKERPFFNDLVSFMISGPVMVQVLEGEGAVLKNRELMGATDPKKADAGT
                     IRADFAESIDANAVHGSDSLENAAIEIAYFFPASEVYAR"
     gene            2699..>3223
                     /locus_tag="PKN55_12910"
     CDS             2699..>3223
                     /locus_tag="PKN55_12910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX72192.1"
                     /translation="MNDTTKPDSNAPKTSAASPAGGKGKPASSKPAGAPPAGGKPPAG
                     STAKPANPNRKRLIIAGIGIAAALAAVPAFNYFNKPKSAATAPTPEVSPTVASGKIKD
                     APPPPPAEAPPPSPVAPDAVSPEEADKISSPPPPPPAPPKPEPAPAPAPHAAPAPPPP
                     KPTAPPPPPTPAAKP"
BASE COUNT          582 a         1051 c         1016 g          574 t
ORIGIN      
        1 tcttgtcggc cagcggcacc gcctggccct tgtaattgag ctccacttgc ggagcattgc
       61 cgatgatcag cttgtacggc ggcgtgccgc cgcccaggcg tttttcgccg ctgatctggc
      121 cgtacagcaa ttttttgccc ttggcgtcga tcacggacac ccaggctttc tcgcgggcgt
      181 tcagcaccag ttcacccgcg ccagtgtcgc tgacggcggc ttgcgccgtg ctggtgtcgg
      241 tgctggtcgg tttggcctct gcgggcttga cctcggccgg cttcaccggt gccacggcaa
      301 gcgcttcgag cttggctggc tcgggtttga gttcggcagg cttgctgtca accgacggag
      361 cggcgctgac cggagcggca gagctggccg gctcaggatt cagcgcggcc tgcggcaagg
      421 cgctgtccac agcaggcgcg gccagcggcg gcgtggccgg ggtgctggcg ccctgggtga
      481 gcaggcgata gcccacgcct gccgccagca gcagcaccag cagagccagt gcgcccttga
      541 tcagggtggg attgacagct ggcgagcggc tggacgccgc cggtgccggt gcggccggtg
      601 cggtgtcagc cacaggcgga ttgaccggcg gcatcggcag atgggcatcc agcgaggcat
      661 tcagcaaggc cacatccagg cccagcagct tggcgtaatt gcgcacaaaa ccacgcgcaa
      721 acgccggacc gggcaatttg tcgtattcgt cgttttcgat ggcttccagt tgcttgcggg
      781 acagcttgag ccggtcggcg acctcttgca aggacaagcc gcgcttgacc cgctcattgg
      841 acagcagcat gccaggagaa gctgtgtgtg tagccgattc agcgccgggc gtggggatat
      901 cgctcattca gtacagtccg cttgcaaaag ggttcaataa aatacagctg ttggcgcaga
      961 cccgccagcg cgggacaagg ttcactccgg cgcagcgtcc gacagccagc gcacggcgcg
     1021 gcgggttttg tcctccacct tgccggccag ctggccacag gcggcgtcga tgtcgtcgcc
     1081 gcgcgtcttg cgcaccgtca ccacataacc cgcttcctgc aggatttccc ggaatgtgcg
     1141 aatggccccg gcgctggagc gggcatagcc ggaattcggg aagggattga acgggatcag
     1201 gttgaacttg cacggcacgg ccttcaccag tgcaagcaat tgccgtgcat gttcgggcgt
     1261 gtcgttcacg ccgtcaagca tcacatattc gaacgtcacg aaatcgcgcg gcgcgcgctg
     1321 caaatagcgt tcgcaggcgg ccagcagttc tcgcaacggg tattttttgt tgatcggcac
     1381 gatctggtcg cgcagcgcgt cgttgggcgc gtgcaggctg accgccagcg ccaccgggca
     1441 atcttcgcgc agccggtcca gcgccggcac caggccggag gtggacaagg tcacgcggcg
     1501 gcggctcagg ccgtagccgt ggtcgtccag catgatctgc atggcggcca ccacattgtc
     1561 gtagttggcc agcggctcgc ccatgcccat catcaccaca ttggaaatca cccgctcgtt
     1621 tttcggggtg acgcccatgg atttgttcgc ccaccacagc tgaccgatga tctcggcggt
     1681 ggacagattg cggttgaagc cctgacggcc ggtggagcaa aacgtgcatt ccagcgcgca
     1741 gcccacctgg ctggacacgc acagcgtgcc ccggtcatct tcggggatga acacggtttc
     1801 cacgccattg cccgtgccca catccagcag ccacttgcgc gtgccgtcgg tggaagcctg
     1861 cccggtcatc agctgcggca cgcgcacttc ggcgcaggcc tccagcttgg cgcgcaaggt
     1921 cttggccaga tcagtcatgg cagaaaaatc atccgtgccc atctggtgca tccagcgcat
     1981 cacttgcgtg gcgcgaaacg gcttctcgcc catggcggcg aaatggtcgg tgagttgcgg
     2041 cagggtaaaa tcgagcagat tggttttcat ggcggggcgt caggttgcgg gccacacacg
     2101 cggcccgcag gggcggaaat cagcgggcgt acacttccga agccgggaag aagtaggcga
     2161 tttcgatcgc ggcgttttcc aggctgtccg aaccgtgcac ggcgttggcg tcgatggatt
     2221 cggcaaagtc agcgcggatg gtgccggcgt cggccttttt cgggtcggtg gcgcccatca
     2281 gctcacggtt tttcagcaca gcgccttcgc cttccagcac ttgcaccatc accgggccag
     2341 agatcatgaa gctgaccaga tcgttgaaga acgggcgctc tttgtgcaca gcgtaaaagc
     2401 cttcggcttc ggcgcgggac aggtgcttca tcttggcggc aaccactttc aggccagcag
     2461 cttcaaagcg gctgtagatt tggccgatga catttttggc gactgcatcg ggcttgacga
     2521 tggacagcgt acgttcgatt gccataaaaa cttctccagg tgaaaagaca aatggttaca
     2581 ctagtaacct gttcgatttt agccgcatca gagttgggct tgtggcccag tcagttgcga
     2641 agaaccctgc attctaacag cttttgcgct ttttgccgct ctcggccgcg atgacgacat
     2701 gaacgacacg accaaaccgg acagcaacgc tcccaaaacc agcgccgcca gccccgccgg
     2761 cggcaagggt aaacccgctt cttccaaacc tgctggcgcg ccgccggcgg gtggcaagcc
     2821 gccggcaggc agcaccgcca aaccggccaa tcccaatcgc aagcggctga ttattgccgg
     2881 catcggcatt gcggctgccc tggcagccgt gccggcattc aattacttca acaagcccaa
     2941 atctgcggca accgccccca ccccggaggt gagccccacc gtggccagtg gcaagatcaa
     3001 ggatgcgcca ccgccgccac cggccgaagc cccgccgccc agcccggtgg cgccggacgc
     3061 ggtaagcccg gaagaagccg acaagatcag cagcccgccg ccaccgccgc cggccccacc
     3121 caaacctgag ccggcgccag caccggcccc tcatgctgca cctgcgccgc caccgcccaa
     3181 gccgactgca ccgccaccgc cgccgacccc ggcagccaaa ccg
//
LOCUS       DAOPYX010000328         3201 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_41061_length_3201_cov_6.819453, whole genome shotgun sequence.
ACCESSION   DAOPYX010000328 DAOPYX010000000
VERSION     DAOPYX010000328.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 3201)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3201)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3201
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41061_length_3201_cov_6.819453"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..785
                     /locus_tag="PKN55_12915"
     CDS             <1..785
                     /locus_tag="PKN55_12915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008953483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HNX72193.1"
                     /translation="NLDAAVLTQANKFDPGNKYAVPYFYGINTLAINVKKVKAALGSE
                     PMPANEWDLLFDQKYASKLKNCGVSVLDSPSEVLPIALHYLGRDPNSHNDADWQAAAE
                     LMKKARPAITRFSSSGYINELANGSICMALGYGGDLNIAKRRAEEAKAGQEIKVLAPK
                     DGVMMWMDSMVIPKDAQNVDNAHAFINYILRPEVAAANAKAVTFASPNAAARSHIDDK
                     FLKDPSIYPTPAIQAVSFLQLPVEAKTQRLQTRLWTEIKTRK"
     gene            932..2044
                     /gene="potA"
                     /locus_tag="PKN55_12920"
     CDS             932..2044
                     /gene="potA"
                     /locus_tag="PKN55_12920"
                     /EC_number="7.6.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011134887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015417 - ABC-type polyamine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter ATP-binding protein"
                     /protein_id="HNX72194.1"
                     /translation="MTPPLADSGKPYLQIVDVVKQFGEHTAVNGVNLTIGKNEIFALL
                     GSSGCGKSTLLRMLAGMETPTSGRILLDGQDVTRLPPYERPINMMFQSYALFPHMTVE
                     QNIAFGLRQDKLSKADINERVARMLELVQMSKYARRKPFQLSGGQQQRVALARSLAKR
                     PKLLLLDEPLGALDKKLRQQTQLELVNMIEQVGVTCIMVTHDQEEAMTMASRVGIMSE
                     GQLLQVASPSEIYERPNCRFTAEFIGETNLFSGQVAVDEADHVQIDSAELRRPIYVGH
                     GITGTLGMPVWVSLRPERVGLSRSAPAGDCNHDQGTVHDIAYLGGFSIYHIQLASGKI
                     VKASVPATRWRDEPAPTWGESVHLSWADNDAVVLTL"
     gene            2041..2967
                     /locus_tag="PKN55_12925"
     CDS             2041..2967
                     /locus_tag="PKN55_12925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014085885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNX72195.1"
                     /translation="MNTPSASPRPRRWLPAGRHLVIAAPYGWLLLFFLLPFLFVLQIS
                     FSEVEIASPPYRPLLSQAEDVLNIALNLGNYRFIFTDPLYFDAYLSSVKVALFTTLWC
                     LLLGYPMAYAIARSAPAQRNTWLMLVMLPFWTSFLVRVYAWMGLLDSEGLINHALLGL
                     GLIDQPLQLLHTAFSVQLVMVYTYLPFMILPLYSNLVKLDGRLLEAAADLGCRPWRAF
                     VTVTLPLSKNGIVAGAMLVFIPAVGEFVIPELVGTPESLMIGKVLWMEFFDNNNWPMA
                     AAVTCVMLALLIAPIIYLHRREEKALARAARH"
     gene            3049..>3201
                     /locus_tag="PKN55_12930"
     CDS             3049..>3201
                     /locus_tag="PKN55_12930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002641105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HNX72196.1"
                     /translation="MQNTSSMSWFGRLSLGSGLAFLYLPIVVLVVYSFNASKLVSVWS
                     GFSLGWY"
BASE COUNT          605 a         1018 c          973 g          605 t
ORIGIN      
        1 aaaacctgga cgccgccgtg ctgacccagg ccaataaatt cgaccccggc aataaatacg
       61 cggtgccgta tttttatggc atcaacaccc tggccatcaa cgtcaaaaaa gtcaaagccg
      121 cattgggcag cgagccgatg ccggccaatg aatgggattt gctgtttgac caaaaatacg
      181 ccagcaagct gaaaaactgc ggggtgtcgg tgctggacag cccgtcggaa gtgctgccga
      241 ttgcgctgca ctacctcggc cgtgacccca acagccataa cgacgccgac tggcaagccg
      301 ccgccgagct gatgaaaaaa gcccgcccgg ccatcacccg cttcagctcg tccggctata
      361 tcaacgaact ggccaatggc tcgatctgta tggcgctggg ctacggcggc gacctgaaca
      421 tcgccaagcg ccgcgccgaa gaagccaagg ccggccagga aatcaaagtg ctggcgccca
      481 aggacggcgt gatgatgtgg atggacagca tggttatccc caaagacgcg caaaacgtcg
      541 ataacgccca cgccttcatc aactacatcc tgcgcccgga agtggccgcc gccaacgcca
      601 aggcggtgac ctttgccagc cccaacgccg ccgcgcgcag ccacattgac gacaaattcc
      661 tgaaagaccc gtcgatttac ccgaccccgg ccatccaggc agtgagcttc ttgcaactgc
      721 cggtggaagc caagacccag cgtttgcaaa cgcgcttgtg gacggaaatc aaaacccgca
      781 agtaagccca ccctgaagcc ggcccggttc ttgccgggcg cgtcgtcccc cggcgacgcg
      841 cccaggaatg actgcccgga cttctcctgc gcgcccctca atttggcggc gcaggcccgg
      901 atttttcttt caggagcgtc acggcatgaa tatgactcct ccgctggcgg acagcggcaa
      961 accgtatttg cagatcgtcg atgtagtcaa acagtttggc gagcacactg ccgtcaacgg
     1021 cgtgaacctg accatcggca aaaatgaaat cttcgccctg ctgggcagct cgggctgcgg
     1081 caaatccacc ctgctgcgca tgctggccgg catggaaacg cccacctccg ggcgcatcct
     1141 gctggatggc caggacgtga cccggctgcc gccgtatgag cggccgatca atatgatgtt
     1201 ccagagctac gcgctgtttc cgcacatgac cgtcgagcag aacatcgcct ttggcctgcg
     1261 ccaggacaag ctgtccaagg cggacatcaa tgagcgggtg gcgcgcatgc tggagctggt
     1321 gcagatgagc aaatacgccc ggcgcaagcc gtttcagctc tccggcggcc agcagcagcg
     1381 cgtggcgctg gcgcgcagcc tggccaagcg ccccaagctc ttgctgctgg acgagccgct
     1441 gggcgcgctg gacaaaaaac tgcgccagca aacccagctg gagctggtga acatgatcga
     1501 gcaagtgggc gtcacctgca tcatggtgac ccacgaccag gaagaagcca tgaccatggc
     1561 cagccgggtg ggcatcatgt cggaaggcca gctgctgcaa gtggccagcc ccagcgaaat
     1621 ctacgagcgg cccaactgcc gcttcaccgc cgagtttatt ggcgaaacca atctgttcag
     1681 cggccaggtg gcggtggatg aagccgacca tgtgcagatc gacagcgccg agctgcgccg
     1741 gccgatttat gtcggccacg gcatcaccgg cacgctgggc atgccggtgt gggtgtcgct
     1801 gcgccccgag cgggtgggcc tgtcgcgcag cgcgccggcc ggcgactgca accacgacca
     1861 gggcacggtg cacgacatcg cctatctggg cgggttttcg atttatcaca tccagctcgc
     1921 cagcggcaaa atcgtcaagg ccagcgtgcc cgccacccgc tggcgcgatg agccggcgcc
     1981 gacctggggc gaaagcgtgc atctgagctg ggccgacaac gacgccgtgg tgctgacgct
     2041 atgaacacac cctccgcctc cccccgccca cgccgctggc tgccagccgg ccgccatctg
     2101 gtgattgccg cgccgtatgg ctggctgctg ctgttttttt tgctgccgtt cttgtttgtg
     2161 ctgcaaatca gcttttccga ggtggaaatc gccagcccgc cttaccggcc gctgttaagt
     2221 caagcggaag acgtgctgaa tattgcgctg aacctgggta actatcggtt tatcttcacc
     2281 gacccgctgt atttcgatgc ctatctgagc tcggtgaaag tggcgctgtt caccacgctg
     2341 tggtgcctgc tgctgggtta tccgatggcc tacgccatcg cccgctcggc cccggcgcag
     2401 cgcaacacct ggctgatgct ggtgatgctg ccgttctgga cctcgtttct ggtgcgggtg
     2461 tacgcctgga tgggcctgct ggacagcgaa gggctgatca accacgcctt gctcggcctg
     2521 gggctgatcg accagccgct gcaactgctg cacacggcgt tctcggtgca gctggtgatg
     2581 gtgtacacct atctgccgtt catgatcctg ccgctgtact ccaacctggt gaagctggat
     2641 ggccgtctgc tggaagccgc cgccgacctg ggctgccggc cctggcgcgc gtttgtcacc
     2701 gtgaccctgc ccttgtcaaa aaacggcatt gtcgccgggg ccatgctggt gttcattccg
     2761 gcagtgggtg agtttgtcat ccccgaactg gtgggcacgc cggaatcgct gatgattggc
     2821 aaggtgttgt ggatggagtt ttttgacaac aacaactggc cgatggccgc tgcagtcacc
     2881 tgcgtgatgc tggcgctgct gattgccccg atcatctacc tgcaccgccg cgaagaaaaa
     2941 gccctggcgc gggcggcccg tcattaatcg tccgtacctg gcccgtgcgg ccaggggcaa
     3001 cggcgccagc cggatggccg gcctggtgtt tgtattgagg aatctgccat gcagaacaca
     3061 tcttccatgt cctggtttgg ccggctgtcg ctgggcagcg ggctggcgtt tctctacctg
     3121 cccattgtgg tgctggtggt gtactcgttc aatgcctcca agctggtcag cgtctggtcg
     3181 gggttttccc tgggctggta c
//
LOCUS       DAOPYX010000329         3192 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_41239_length_3192_cov_2.302200, whole genome shotgun sequence.
ACCESSION   DAOPYX010000329 DAOPYX010000000
VERSION     DAOPYX010000329.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 3192)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3192)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3192
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41239_length_3192_cov_2.302200"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            88..1926
                     /locus_tag="PKN55_12935"
     CDS             88..1926
                     /locus_tag="PKN55_12935"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNX72197.1"
                     /translation="MTPWLSRPAAQHAMRLALALLACLLAAWVEWQRPVLPTRLDEAL
                     RDRLLHLSADPAPDTRVAVIDIDETSLRQLGPWPWPRQRMADLLEILLSHYQVRAIGV
                     DILFPEPADPVGDARLAMLAAHAPVTLAQILDYPERDTPLRQGALAGGLPADPAQPAL
                     GAQGYLANHAGLSATARCLGNIGYQPDADGVLRRLPVLTRYQGRDYPMFAATLIDCAR
                     APNAASVLPLAAGASWRSPYPRAWSAYTVAPAAEVLAQRLPTQLLAGRYVLVGSSSLS
                     LGDHVSTPLAPLNAGVLVHANALSALLDIQAGRGGLPWSGRAGLAAWVLLSVALLAWA
                     MPRVPAWGNIAGLAALTAGWLALGLWGARHGAEWSLSAPLAAYLCLLATAIPFEWWGA
                     QQRNKRLLSTFSRYVGPSVLNEMVRRGLEDSLTPTQREVTVLIADMEGFTRTTAALPL
                     TDAARLTRDFLQRLTGPVLAHGGTLDKYTGDGLVAFGGAPLPCPEQADQAAAAALDIV
                     AAVAALNQQRHADGLPTLRVRIGIESGLALVGDLGTSFRSSYTAIGDCINFASRLESA
                     ARTLPADIIIGAAAAQRIRAHTLRPLGSLQLRGAEVMMEVFTVEPR"
     gene            complement(2004..3146)
                     /locus_tag="PKN55_12940"
     CDS             complement(2004..3146)
                     /locus_tag="PKN55_12940"
                     /inference="COORDINATES: protein motif:HMM:NF013237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aspartate aminotransferase family
                     protein"
                     /protein_id="HNX72198.1"
                     /translation="MTDSLHPATHAAHGGEAPRAPFYDVAPPLHLSTTFTRNAEGGYP
                     GGRVYSRDQSPAYDGPERLLAELEGGEQALLFSSGMAAASAVLHALTPGQRVLAPRAL
                     YWALRNWLREFCADWGLTLDWYANDDLDELARLIQAAPTHLLWIETPANPSWEVTDIA
                     AAAALARAAHARTVVDSTVATPVLTQPLALGADIVLHSATKTLNGHSDVVAGALVCAR
                     QDDPFWQRVRRARALGGAVLGPFEAWLLQRGMRTLFVRARAANANAQQVAEWLAAHPA
                     VASVSYPGLASHPGHAVAARQMRGGFGAMLSFRLRDGEARAKAVAGQLKVFRQATSLG
                     AVESLVEHRASVEGADSPVPRDLLRLSIGIEAVEDLLADLRQALGQ"
BASE COUNT          458 a         1141 c         1111 g          482 t
ORIGIN      
        1 gctgggcgcg ctgcgcggca gaaacccgcg cggtgtaccg ccaggtggct gcccgccacg
       61 gcctgccgga ctgatcggcc ccgccgcatg acgccctggc tctcccgccc ggccgcccag
      121 cacgccatgc gcctggcgct ggcgctgctg gcctgcttgc tggccgcctg ggtggaatgg
      181 cagcgcccgg tgctgcccac ccggctggac gaagccctgc gcgaccgcct gctgcacctc
      241 agcgccgacc ctgccccaga tacccgcgtg gcggtgatcg acatcgacga aaccagcctg
      301 cgccagctgg ggccatggcc gtggccgcgc cagcgcatgg ccgatttgct ggaaatcctg
      361 ctcagtcatt accaggtgcg cgccattggc gtggatattt tgtttcctga gccggccgac
      421 ccggttggcg atgcccgcct ggccatgctg gccgcgcatg cgccggtcac gctggcgcag
      481 attctggatt accccgagcg cgacaccccg ctgcgccagg gcgcgctggc tggcggcttg
      541 ccggccgatc ctgcccagcc ggcgctgggc gcgcagggtt atctggccaa ccacgccggc
      601 ctgtcggcca ccgcgcgctg cctgggcaat atcggctacc agcccgacgc cgacggcgtg
      661 ctgcgccgcc tgccggtgct cacccgctat cagggccgtg actacccgat gtttgccgcc
      721 acgctgatcg actgcgcccg tgcgcccaac gcagcctcgg tattgccctt ggccgcgggc
      781 gccagctggc gctcgcccta tccgcgcgcc tggtcggcct ataccgtggc gcccgccgcc
      841 gaggtgctgg cccagcgtct gcccacacaa ctgctggccg gccgctatgt gctggtgggc
      901 tcgtcctcgc tgagcctggg cgaccatgtc agcacgccgc tggcgccgct gaacgccggc
      961 gtgctggtgc acgccaatgc cctgtcggcg ctgctggata ttcaggccgg gcgcggcggg
     1021 ctaccctggt cggggcgcgc cgggctggcg gcctgggtgt tgctcagcgt cgccttgttg
     1081 gcctgggcca tgccgcgtgt gccggcctgg ggcaatatcg ccggcctggc ggcgctgacc
     1141 gccggctggc tggcgctggg gctgtggggg gcgcggcatg gggcggagtg gtcgttatcg
     1201 gcgccgctgg cggcctatct gtgtctgctg gccacggcga ttccgtttga atggtggggc
     1261 gcccagcagc gcaacaagcg gctgctgagc acgttttccc ggtatgttgg cccgtcggtg
     1321 ctcaacgaga tggtgcgccg tggcctggaa gacagcctga cgcccaccca gcgcgaagtc
     1381 accgtgctga ttgccgatat ggaaggcttt acccgcacca cggcggcgct gccgctgacc
     1441 gacgccgccc ggctgacccg tgattttctg caacggctga ccggcccggt gctggcgcac
     1501 ggcggcacgc tggacaaata caccggagat ggcctggtgg cattcggggg cgcgccactg
     1561 ccctgcccgg aacaggccga ccaggccgcc gctgccgcgc tggatatcgt ggccgccgtg
     1621 gcggcgctga accaacagcg ccacgccgac ggattgccca cgctgcgcgt gcgcattggc
     1681 atcgaaagcg gcctggcgct ggtgggggat ttggggactt cgtttcgcag cagttacacc
     1741 gccatcggcg actgcatcaa ctttgcctca cggctggaat ccgccgcgcg caccctgccg
     1801 gccgacatca tcatcggcgc cgccgccgcc cagcgcattc gcgcgcatac cttgcgccca
     1861 ctgggcagcc tgcaactgcg cggcgctgag gtgatgatgg aagtgttcac cgtcgagccg
     1921 cgctgaacgc aacgtgcctg gcgggcgcgt ggcggccaac aggacacgca cgcacaagca
     1981 cgccagaccg gttttgtgag cggctactgg cccagcgcct ggcgcaaatc ggccagtaaa
     2041 tcttccaccg cctcaatgcc gatcgacagc cgcagcaaat ccctgggcac tggcgaatcc
     2101 gcgccctcta cgctggcgcg gtgttccacc aggctttcca ccgcacccag cgaggtggcc
     2161 tgacggaata ccttgagctg ccccgccacc gccttggcgc gggcttcgcc gtcgcgcagg
     2221 cggaacgaca acatcgcgcc aaatccgccg cgcatctgcc gggcggccac cgcatggccg
     2281 gggtggctgg ccaggccagg gtagctgacc gaagccaccg ccggatgggc ggccagccat
     2341 tccgccacct gctgggcgtt ggcgttggcg gcgcgggcgc gcacaaacag cgtgcgcatg
     2401 ccgcgctgca ataaccaggc ctcaaacggc cccagcaccg cgccgcccag cgcgcgggcg
     2461 cggcgcaccc gctgccagaa cgggtcgtcc tgacgggcgc acaccagcgc gccggccacc
     2521 acgtcggaat ggccgttcag ggttttggtg gccgaatgca gcacgatgtc ggcgcccagc
     2581 gccagcggct gggtcagcac tggcgtggcc acggtggaat ccaccaccgt gcgtgcgtgc
     2641 gccgcgcgcg ccagcgcagc ggcggcggca atgtcggtga cttcccaact cgggttggcc
     2701 ggggtttcaa tccacagcaa atgggtgggc gcggcctgaa tcagccgggc cagctcgtcc
     2761 aggtcgtcat tggcgtacca gtccagcgtt agcccccagt cggcgcaaaa ttcgcgcaac
     2821 caattgcgca gcgcccaata cagcgcgcgc ggcgccagca cgcgctggcc aggggtgagc
     2881 gcgtgcagca cggcgctggc ggcggccatg cccgaggaaa acaacaaagc ctgttcgccg
     2941 ccttccagct cggccagcaa gcgctcgggg ccatcgtagg ccgggctttg atcgcgtgaa
     3001 tacacccgcc cgcccggata gccgccctcg gcgttgcggg tgaaggtggt ggacaggtgc
     3061 agcggcgggg ccacatcgta aaacggcgcg cgtggcgctt cgccgccatg ggcggcgtgg
     3121 gtggcggggt gcaaggaatc agtcatgggt aggcgcgggc aaaaaagaca gaaagcatcg
     3181 ccagcccgca gg
//
LOCUS       DAOPYX010000330         3182 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_41412_length_3182_cov_10.034218, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000330 DAOPYX010000000
VERSION     DAOPYX010000330.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 3182)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3182)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3182
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41412_length_3182_cov_10.034218"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1707
                     /locus_tag="PKN55_12945"
     CDS             <1..1707
                     /locus_tag="PKN55_12945"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNX72199.1"
                     /translation="TVAVAVVLSGVVALTLTPALCALLLKAQHTENKLFAPFNRAFDR
                     LTNRYTGTVRFTLRHGVVGVLVFAGVVGATALLFKLIPGGFVPAEDQGRLISALSLPD
                     GATLKRTEATGERMRQMVAKDPAVEHTFIVSGFDLISGGNKTNAGTVFVTLKDWSERE
                     GKAQDMVGKFTGFGMSQPDGMALVFNPAPIQGLGTTGGFEVYVQNRVDGDPKKLSEVL
                     NVLMDELKKHPEFTRISTFFRPTVPQLFVEVDEQKAMALGIAISDVYTTLQSTMGAYY
                     VNDFNKAGRVYRVQLQAEAPYRMKPDDINRVYVKSQSSQAMIPLSAIATVKNVVGPEQ
                     VERFNGFIAAKLMGDSKPGVSSGQAMQIVEDVAAATLPEGYSIAWTGQAFQEKRSAGS
                     SLQAFGFAIIMVFLILAAQYEKWSLPVAVVMSVPFALMGALTAIWLRGMPNDIYFQIG
                     LVVLIGLASKNAILIVEFAAQKYREGLSAADAAIEAARLRLRPIIMTSLAFVLGVFPL
                     VKATGAGAGARQSMGTGVFGGMLAATFIATLFVPLFFKWLERGKQIDPSHMDADDEEK
                     HP"
     gene            1704..3113
                     /locus_tag="PKN55_12950"
     CDS             1704..3113
                     /locus_tag="PKN55_12950"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HNX72200.1"
                     /translation="MMARISPLAAALTALTLAGCAIGPDYHRPSAALPASFDASPTRA
                     ASAAVQADWWVLFQDPTLNQLVERALSHNADVRLAVARVEQADAVAREAGAAFLPEID
                     GGASASKTQASTRTASYSANSPRLRTARSATLSTSYELDVWGRVRRANESVRASLLAS
                     QYARDAVRLSVAGVVTSTYLNLRALDAQLAVVGETVRTREEGQRLMQLRLNAGLSSLV
                     DSQAADNALHAARAQLAELRRQRALSEHQLALLSGQPELRLAAGDVRQLPLPPQPPAG
                     LPAELIEARPDVMQAEQQLIAANANIGVAKAGYFPRFTLTGSTGSESQTLADLFSAGA
                     GAWSLGISALMPILDFGRTSARVDQARALNQQSLISWQNALQVAYKDVRDALVNLRED
                     GEAERAQASRVANARHTLGLAHLRLQAGHASLLDVLDSQRTFNDAQLAYITTRQAQLN
                     ASVELFKALGGGWKPATGS"
BASE COUNT          549 a         1018 c         1064 g          551 t
ORIGIN      
        1 accgtggcgg tggcggtggt gctgtccggc gtggtggcgc tgacgctgac cccggccttg
       61 tgcgcgctgc tgctgaaagc ccagcacacc gaaaacaagc tgtttgcccc gttcaaccgc
      121 gcgtttgacc ggctgaccaa tcgctatacc ggcacggtgc gttttaccct gcgccatggg
      181 gtggtgggcg tgctggtgtt tgccggtgtg gtgggcgcca ccgcgctgtt gttcaagctg
      241 atccccggcg gctttgtgcc ggccgaggat cagggccggc tgatttcggc cttgagcctg
      301 cccgacggcg ccacgctcaa acgcaccgaa gccacgggcg agcgtatgcg ccagatggtg
      361 gccaaagacc cggcggtgga acacaccttt attgtctcgg gttttgactt gatcagcggc
      421 ggcaataaaa ccaacgctgg cacggtgttt gtcaccctga aagactggtc ggagcgcgaa
      481 ggcaaggcac aagacatggt gggcaaattc accggttttg gcatgagcca gcctgatggc
      541 atggcgctgg tgttcaaccc ggcgccaatc cagggcctgg gcaccaccgg cggttttgaa
      601 gtgtatgtgc aaaaccgggt ggatggcgat ccgaaaaagc tgagcgaggt gctgaacgtc
      661 ttgatggacg aactgaagaa acacccggaa ttcacccgga tttctacttt cttccgcccc
      721 accgtgccgc agctgtttgt cgaagtggac gaacaaaaag ccatggcgct gggcattgcc
      781 atctcggatg tgtacaccac gctgcaaagc accatgggcg cgtactacgt caacgacttc
      841 aacaaggccg gccgggtata ccgggtgcag ttgcaagccg aagcgccgta ccgcatgaag
      901 ccggatgaca tcaaccgggt gtacgtcaag agccagagca gccaggcgat gattccgctg
      961 tcggccatcg ccacggtgaa aaacgtggtt ggccccgagc aggtggagcg ctttaacggc
     1021 tttatcgccg ccaagctgat gggcgacagc aagccggggg tgagctcggg ccaggcgatg
     1081 cagattgtcg aagacgtggc cgccgccacc ctgcccgagg gctacagcat tgcctggact
     1141 ggccaggcct tccaggaaaa acgcagtgcc ggctcctcgc tgcaagcctt tggctttgcc
     1201 atcatcatgg tgtttttgat tctggccgcc cagtatgaaa aatggtcgct gcccgtcgcc
     1261 gtggtgatgt cggtgccgtt tgcgctgatg ggcgcgctca ccgccatctg gctgcgcggc
     1321 atgcccaacg atatttactt ccagattggc ctggtggtgc tgatcgggct ggcgtcgaaa
     1381 aacgccatcc tgattgtcga gttcgccgcg caaaaatacc gcgaaggcct gagcgcggcc
     1441 gatgccgcca tcgaggcggc gcggttgcgg ctgcggccca tcatcatgac ctcgctggcc
     1501 tttgtgctgg gcgtgttccc gctggtcaaa gccactggcg ccggcgcggg cgcgcgccag
     1561 tcgatgggca ccggggtgtt tggcggcatg ctggctgcca cctttatcgc cacgctgttt
     1621 gtgccgctgt tcttcaagtg gctggagcgc ggcaagcaaa tcgacccgtc gcatatggat
     1681 gccgacgacg aggagaaaca cccatgatgg cgcgcatttc ccctctggcc gccgcactga
     1741 cggcgctgac cctggcgggc tgcgccatcg ggccggacta ccaccgcccc agcgccgcgc
     1801 tgccggccag ctttgacgcc tctcccaccc gcgccgccag cgcggcggtg caggccgatt
     1861 ggtgggtgct gtttcaggac ccgacgctta accagctggt cgagcgcgcc ttgagccaca
     1921 atgccgacgt gcggctggcg gtggcgcggg tggagcaggc cgacgccgtg gcgcgtgagg
     1981 ccggtgcggc gtttttgcca gaaatcgacg gcggcgccag cgccagcaaa acccaggcga
     2041 gcacccgcac cgccagctac tcggccaatt cgccgcgcct gcgcaccgcg cgcagcgcca
     2101 cgctgagcac ttcttacgag ctggacgtgt ggggccgggt gcgccgcgcc aatgaatcgg
     2161 tgcgcgccag cctgctggcc agccagtacg cgcgcgacgc cgtgcgcctg tcggtggccg
     2221 gcgtggtgac cagcacctat ctgaatttgc gcgcgctgga tgcgcaattg gcggtggtag
     2281 gcgaaaccgt gcgcacgcgc gaggaaggcc agcggctgat gcagctgcgg ctgaatgccg
     2341 gcctcagctc gttggtggat agccaggcag cggataacgc cctgcacgct gcccgcgccc
     2401 aactggccga gttgcgccgc cagcgcgcct tgtccgaaca ccagttggcc ctgctgagcg
     2461 gccagccaga actgcgcctg gccgctggcg atgtgcgcca gctgccactg ccgccgcaac
     2521 cgccggccgg cctgccggcc gagctgatcg aagcccggcc ggacgtgatg caggccgagc
     2581 aacagctgat tgccgccaac gccaatatcg gcgtggccaa ggccgggtat ttcccgcgtt
     2641 ttaccctcac cggcagcacc ggcagcgaaa gccagaccct ggcggatttg ttcagtgccg
     2701 gcgcgggcgc gtggtcgctg gggatttcgg cattaatgcc gattctggat tttggccgca
     2761 cctcggcacg ggtggatcag gcacgcgcgc tgaatcagca atcgctgatc agttggcaaa
     2821 acgccctgca agtggcctac aaagacgtgc gcgatgcgct ggtgaatctg cgtgaagatg
     2881 gcgaggccga gcgcgcccag gccagccggg tggccaacgc ccgccacacc ctgggcctgg
     2941 cgcacctgcg cctgcaagcc ggccacgcca gcctgctgga cgtgctggac agccaacgca
     3001 cctttaacga cgcccaactg gcgtacatca ccacccgcca ggcgcagctg aacgccagcg
     3061 tcgagctgtt caaggcgctg ggtggcggct ggaagccggc gactggcagt taagcgccgc
     3121 gcgacagtgg gcgcccaact cagcgcggtg cgtctggcca ggcgcaccgc gctgggtttt
     3181 gt
//
LOCUS       DAOPYX010000331         3179 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_41464_length_3179_cov_13.922215, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000331 DAOPYX010000000
VERSION     DAOPYX010000331.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 3179)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3179)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3179
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41464_length_3179_cov_13.922215"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1918)
                     /gene="recD"
                     /locus_tag="PKN55_12955"
     CDS             complement(<1..1918)
                     /gene="recD"
                     /locus_tag="PKN55_12955"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HNX72201.1"
                     /translation="MTHPAPLLDIQPLLDDWLARRWLRPLDVRFAAFLAELDPDADPR
                     VWLAGALASHQLGRGHPCLDLRALGQHASLEDALGLPPEGAPRAANALMPPALWPWPA
                     AATPLLAEADADAGRASPLVRDGARLYLRRYWQYEQDVAAALCARAAPLSAAAQPTLP
                     DWLRASGAKPDWQTLACALALRGRLTLITGGPGTGKTTTVVRVLALLQHQAIAAGAPP
                     LRLRLAAPTGKAAARLSESLESARQRLVGLAPDEVLTHIPLTVSTVHRLLGSRPDSRH
                     FRHHREAPLHADVVVVDEASMIDLEMMAALLDALPEHARLILLGDKDQLASVEAGAVL
                     GQLCAHADAGGYRPDTLNWLAAAADGADLGPYAARPELAAPPLADCTVMLRHSHRFDA
                     SSGIGQLAQAVRQGAELGALWGRYADIRCLRLHGEHDAQLDQLLRNGYQAMLHAVRQR
                     PAADAAPADWDAWAQHALRVFAGFQLLTPLRRGPWGVAGLNARAEQVLAAAGLIQPER
                     EWYPGRPVMLTRNDPTLGLMNGDVGLTVLDARQQLRVIFAQAGAVRWISPLRLPDVDT
                     VFAMTVHKSQGSEFTHAALILPDHPSPLLTRELAYTAITRAKAEFTLLEAKEGMLALA
                     AGTETRRQSGLGERV"
     gene            complement(1959..>3179)
                     /locus_tag="PKN55_12960"
     CDS             complement(1959..>3179)
                     /locus_tag="PKN55_12960"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HNX72202.1"
                     /translation="AQDRERLQEDLRLFYVAVTRARHALWLGIAPLRRGRGKSNTLHE
                     SALGHLLNGGAPIDDAAVPALCRDWLDAEHAAASELAWAELDPSLAPARLPPPADAPA
                     LAAARACRLRPPAPWRISSYSGLTLRLDAEAAPLAESAHDDVAQEAQADAEREPASAV
                     GGLPALLNALPPGAASGVFVHSLLEWMAHTGFAAVAANPSALRDRVARGCALRQWPQL
                     IDPLTEQLLAWLHTPLGLPGAPATLAGLGAYQAELEFWFAAQQLPLPALDAHTRAMLL
                     PGLPRPALAADTLNGMLKGFADLVLCHDGRYYVVDYKTNWLGPLRADYAPAALAAPLA
                     ESRYDLQAAIYLLALHRHLRERLPDYQCERHLGGALYLFLRGWDGHGHGVWHACPSAA
                     EMDALDQLFAGGRA"
BASE COUNT          426 a         1075 c         1256 g          422 t
ORIGIN      
        1 ccaccctctc ccccaaccca ctctgccgcc tcgtctccgt ccccgccgcc agcgccagca
       61 tcccttcttt tgcctccagt aaagtaaatt ccgccttggc gcgggtaatc gccgtgtagg
      121 cgagttcgcg ggtgagcagg gggctggggt ggtcgggcag gatcagggcg gcgtgggtga
      181 attccgagcc ttgtgatttg tgcacggtca tggcgaacac ggtgtccacg tcgggcaggc
      241 gcagcggcga gatccagcgc acggcgccgg cttgggcgaa gatcacccgc agttgctggc
      301 gggcgtccag cacggtcagg ccgacgtcgc cgttcatcag gcccagggtg gggtcgttgc
      361 gggtgagcat caccgggcgg ccgggatacc attcgcgttc gggctggatc aggccggcgg
      421 cggccagcac ctgttcggcg cgggcgttca ggccggccac gccccagggg ccacggcgca
      481 gcggggtgag cagctgaaaa ccggcaaaca cccgcagggc gtgttgcgcc caggcgtccc
      541 aatcggctgg ggcggcgtcg gcggctgggc gttggcgcac ggcgtgcagc atggcttgat
      601 agccgttgcg caaaagttga tccagctggg cgtcgtgttc gccgtgcagg cgcagacagc
      661 ggatgtcggc gtagcggccc cacagcgcgc ccagctcggc gccctggcgc acggcttgcg
      721 ccagttggcc gatgccgctg ctggcgtcaa agcggtgcga gtggcgcagc atcaccgtgc
      781 agtcggccaa gggcggcgcg gccagttcgg ggcgggcggc gtaggggccc agatcagcgc
      841 cgtcggcggc ggcggccagc cagttcaggg tgtcggggcg gtagccgccg gcatcggcgt
      901 gggcgcacag ctggcccagc acggcgccgg cttcgacaga ggccagttgg tctttgtcgc
      961 ccagcaggat cagccgggcg tgttccggca gcgcatccag cagcgcggcc atcatttcca
     1021 gatcaatcat cgaggcttcg tccaccacca ccacgtcggc gtgcagcggc gcttcgcggt
     1081 gatggcgaaa atgccggctg tccgggcggc tgcccagcag gcggtgcacg gtgctgacgg
     1141 tcagcgggat gtgcgtgagc acctcgtccg gggccaggcc caccaggcgc tggcgggcgg
     1201 attccagcga ttcgctcagc cgggcggcgg ccttgccggt gggcgcggcc aggcgcaggc
     1261 gcaacggcgg cgcgccagcg gcgatggcct ggtgttgcag cagcgccagc acgcgcacca
     1321 cggtggtggt tttgccggtg ccggggccgc cggtgatcaa ggtcagccgg ccgcgcagcg
     1381 ccagcgcgca ggccagggtt tgccagtcgg gcttggcgcc gctggcgcgc agccagtcgg
     1441 gcagcgtggg ttgcgcggcg gcgctcagtg gcgcggcgcg ggcgcacagc gcggcggcca
     1501 cgtcttgttc gtattgccaa tagcggcgca gatacagccg cgcgccgtcg cgcaccagcg
     1561 gcgaggcgcg gccggcgtcg gcgtccgcct cggccagcaa gggcgtggcg gcggccggcc
     1621 acggccacag cgcgggcggc atgagcgcat tcgcggcgcg cggcgcgcct tctggcggca
     1681 ggcccagcgc gtcttccagc gaggcgtgct ggcccagcgc gcgcaaatcc aggcaggggt
     1741 ggccacgccc cagctggtgg ctggccaagg cgccggccag ccacacgcgc gggtcggcgt
     1801 cggggtcaag ctcggccaaa aacgcggcaa agcgcacgtc cagcggccgc agccagcggc
     1861 gcgccagcca gtcgtcgagc aggggctgga tgtccagcag cggggcgggg tgggtcatgg
     1921 cgcatccttg gcggcaggcg ggcaaatacg gcgggggctc atgcgcggcc cccggcaaac
     1981 agctggtcca gcgcgtccat ctcggcggcg ctggggcagg catgccacac gccgtggccg
     2041 tggccgtccc agccgcgcaa aaacagatac agcgcgccgc ccaggtggcg ttcgcactgg
     2101 tagtcgggca ggcgttcgcg caggtggcgg tgcagcgcca gcagataaat cgccgcttgc
     2161 aggtcgtagc gcgactcggc cagcggcgcg gccagcgcgg ccggggcgta gtcggcgcgc
     2221 agcgggccca gccagttggt tttgtaatcc accacgtaat agcggccgtc gtgacacagc
     2281 accaggtcgg caaagccttt gagcatgccg ttgagcgtgt cggcggccag cgccgggcgc
     2341 ggcaggccgg gcagcagcat ggcgcgggtg tgggcgtcca gcgccggcag cggcagctgc
     2401 tgggcggcaa accagaattc cagttcggcc tgataggcgc ccagcccggc cagggtggcc
     2461 ggcgcgccgg gcaggcccag cggggtgtgc agccaggcca gcagttgttc ggtcagcggg
     2521 tcgatcagtt gcggccattg gcgcagcgcg cagccgcgcg ccacgcggtc gcgcagcgcc
     2581 gacgggttgg cggccacggc ggcaaagccg gtgtgcgcca tccattccag caggctgtgc
     2641 acaaacacgc cgctggcggc gccgggcggc agcgcattca gcagcgccgg caggccgccc
     2701 acggcgctgg ccggctcgcg ctcggcgtcg gcctgggctt cctgggccac gtcgtcatgg
     2761 gcggattcgg ccagcggcgc ggcctcggcg tccaggcgca gggtcagccc gctatagctg
     2821 ctgatgcgcc agggcgcagg cgggcgcagc cggcaggcgc gggcggcggc cagcgccggg
     2881 gcgtcggcgg gcggcggcag gcgcgccggg gccaggctgg ggtcaagctc agcccaggcc
     2941 agttcgctgg cggcggcgtg ttcggcgtcc agccagtcgc ggcacagcgc cggcacggcg
     3001 gcatcgtcga tgggcgcgcc gccgttgagc aggtggccca gcgcgctttc gtgcagggtg
     3061 ttgctcttgc cgcgcccgcg cctgagcggg gcgatgccca gccacagcgc gtggcgggcg
     3121 cgggtgaccg ccacataaaa cagccgcagg tcttcttgca ggcgctcgcg gtcttgcgc
//
LOCUS       DAOPYX010000332         3162 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_41805_length_3162_cov_7.520760, whole genome shotgun sequence.
ACCESSION   DAOPYX010000332 DAOPYX010000000
VERSION     DAOPYX010000332.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 3162)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3162)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3162
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41805_length_3162_cov_7.520760"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            135..1721
                     /gene="urtB"
                     /locus_tag="PKN55_12965"
     CDS             135..1721
                     /gene="urtB"
                     /locus_tag="PKN55_12965"
                     /inference="COORDINATES: protein motif:HMM:TIGR03409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter permease subunit UrtB"
                     /protein_id="HNX72203.1"
                     /translation="MRSRLLHAILPWLALLMCWPAWADSPAQALVQADAGARAELIER
                     WAAEGQPAQAALMQALAEGRLYQNADGKAYVQTDAGFADADSGQAVKVDESTLEAIPL
                     NNRVRAALDIAHAARALSAPEASARLAALRTLADASSSALLPLLDAQLKQETDPAVRA
                     AIGQVSANLRLSHADAAQRKQAVIALADSADPAVRTRLQALTEPANEPDASVRAAAAS
                     SVKAMDSRLFMFELLGHAFSGVSLGSVLLLAALGLAITYGLLGVINMAHGEMLMLGAY
                     STYVVQTLFRQYWPEQFGAYLIAALPVAFVVTALIGMALERLVIRHLYGRPLETLLAT
                     WGISLMLMQSVRVLFGAQNVEVANPDWLSGGWQLNAALTLPYNRIAIIGFVLAVLLLT
                     WLLLNKTRLGLFVRAVTQNRRMADCVGVPTGRVDMLAFGLGSGIAGLGGVALSQIGNV
                     GPDLGQGYIVDSFMVVVLGGVGQLAGTVVGAMGLGMVNKILEPQVGAVLGKIAILAFI
                     ILFIQKRPQGLFALKGRVID"
     gene            1732..2829
                     /gene="urtC"
                     /locus_tag="PKN55_12970"
     CDS             1732..2829
                     /gene="urtC"
                     /locus_tag="PKN55_12970"
                     /inference="COORDINATES: protein motif:HMM:TIGR03408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter permease subunit UrtC"
                     /protein_id="HNX72204.1"
                     /translation="MHQPYSQRLIGALGPAQARLLGGLALASLVALPIMHLLPESHAL
                     HVSAYTLTLAGKILCYAIVALAMDLVWGYTGLLSLGHGLFFALGGYGMGMYLMRQIGR
                     DGNYQSDLPDFMVFLDWKTLPWYWQGTDSFVWSMLLVVLVPGLLALGFGWLAFRSRIK
                     GVYFSIMTQALTFAAMLLFFRNETGFGGNNGFTDFKRILGFPVAAPATRAVLFLLTVL
                     LLVLALYGGWKLTRSKFGRILTAVRDAESRLMFCGYNPLGYKLFIWVLSAVICGVAGA
                     LYVPQVGIINPSEMSPGNSIEIAIWVAVGGRGTLIGPLLGAGLINLAKSWFTVAFPEY
                     WLFFLGFLFIAVTLFLPRGVMGLLTRKGAST"
     gene            2826..>3162
                     /locus_tag="PKN55_12975"
     CDS             2826..>3162
                     /locus_tag="PKN55_12975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019919716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNX72205.1"
                     /translation="MSPRTDAATGEDFGWEGEATGLARPVDASQLDTRHGDILYLDDI
                     TVSFDGFRALNKLTLDIAVGELRCIIGPNGAGKTTMMDVITGKTRPDAGTAFFGQTID
                     LTRLNEPTIA"
BASE COUNT          477 a         1017 c         1052 g          616 t
ORIGIN      
        1 tccctggcca cagggatggg ctggtgcggc atacaccccg ccgaggctgg cccgccgtgc
       61 atcccgctgc acggttgtgt ttgtgcgggg cgctggccgc cccgtttttt ttgaattttt
      121 ccaaggattt gggcatgcgt tcacgcttat tgcacgccat tttgccgtgg ctggcgctgc
      181 tgatgtgctg gccggcctgg gcggatagcc cggcgcaggc gctggtgcaa gccgacgccg
      241 gcgcgcgcgc cgaattgatc gaacgctggg cggccgaagg ccagccggcg caggcggcct
      301 tgatgcaggc gctggccgaa ggccggctgt accaaaacgc cgacggcaag gcctatgtgc
      361 aaaccgacgc cggttttgcc gacgctgaca gcggccaggc ggtgaaggtg gacgaaagca
      421 cgctggaagc catcccgttg aataaccggg tgcgcgccgc gctggacatc gcccacgccg
      481 cgcgcgcctt gagcgcgcca gaagccagcg cccggctggc ggcgctgcgc accctggccg
      541 acgccagctc cagcgcgctg ctgccgctgc tggatgcgca actgaaacaa gaaaccgacc
      601 cggcggtgcg cgccgccatc ggccaggtca gcgccaatct gcgcctgagc catgccgacg
      661 ccgcgcagcg caaacaagcg gtgattgcgc tggccgacag cgccgacccg gcggtgcgca
      721 cccgcctgca agcgttgacc gagccggcca acgagcccga cgccagcgtg cgcgccgccg
      781 ccgccagcag tgtcaaggcc atggacagcc gcctgttcat gttcgagctg ctcggccacg
      841 ccttcagcgg cgtcagcctg ggttcggtgc tgctgctggc ggcgctgggc ctggccatca
      901 cctatggcct gctgggggtg atcaatatgg cgcacggcga gatgctgatg ctgggcgcgt
      961 attccactta tgtggtgcaa acgctgtttc gccaatactg gcccgagcag tttggcgcct
     1021 acctgatcgc cgcgctgccg gtggcgtttg tggtgactgc gctgatcggc atggcgctgg
     1081 agcggctggt gattcgccat ctgtatggcc gcccgctgga aaccctgctg gccacctggg
     1141 gcatcagcct gatgctgatg caaagcgtgc gcgtgctgtt tggcgcgcaa aacgtggaag
     1201 tcgccaaccc ggactggctg tctggcggct ggcagctcaa cgccgcgctg accctgccgt
     1261 ataaccgcat cgccattatc ggctttgtgc tggcggtgct gctgctcacc tggctgctgc
     1321 tgaacaaaac ccggctgggg ctgtttgtcc gcgcggtgac gcaaaaccgc cgcatggccg
     1381 actgcgtggg cgtgcccacc ggccgggtgg acatgctggc ctttggcctg ggctcgggca
     1441 ttgccgggct gggcggggtg gcgttaagcc agattggcaa tgtcggcccc gacctgggcc
     1501 agggttatat cgttgacagc ttcatggtgg tggtgctggg cggcgtcggc caactggccg
     1561 gcaccgtggt gggggcgatg ggcctgggca tggtcaataa aatcctcgag ccgcaagtgg
     1621 gcgccgtgct gggcaaaatc gccatcctcg ccttcatcat cctgttcatc cagaaacgtc
     1681 cgcaaggcct gtttgccctc aagggccggg tgatcgacta ggagccagag catgcatcaa
     1741 ccgtattcac aacgcctgat tggcgcactc ggcccggcgc aggcgcgcct gctgggcggg
     1801 ctggcgctgg ccagcctggt cgccctgccc atcatgcacc tgttgccaga gagccacgcc
     1861 ctgcatgtgt cggcctacac gctgaccctg gccggcaaga ttctgtgcta cgcgattgtc
     1921 gcgctggcca tggatctggt gtggggctac accggcctgc tcagcctggg ccatgggctg
     1981 ttttttgccc tgggcggcta tggcatgggc atgtatctga tgcgccagat tggccgcgac
     2041 ggcaattacc aaagcgacct gccggacttc atggtgtttc tcgactggaa aaccctgccg
     2101 tggtactggc aaggcaccga cagctttgtc tggtccatgc tgctggtggt gctggttcct
     2161 ggcctgttgg cgctgggctt tggctggctg gcgtttcgct cgcgcatcaa gggggtgtat
     2221 ttctcgatca tgacccaggc gctgaccttt gccgccatgt tgctgttttt tcgcaacgaa
     2281 accggctttg gcggcaataa cggctttacc gacttcaagc gcattctggg ctttccggtg
     2341 gcggccccgg ccacccgcgc cgtgctgttt ttgctcaccg tgctgctgct ggtgctggcg
     2401 ctgtacggcg gctggaagct cacccgctcc aaattcggcc gcatcctcac cgccgtgcgc
     2461 gacgccgaat cgcggctgat gttctgcggc tacaacccgc tgggctacaa gctgttcatc
     2521 tgggtgctgt cggcggtgat ttgcggcgtg gccggcgcgc tgtacgtgcc gcaggtgggc
     2581 attatcaacc cctcggaaat gtcgccgggc aactcgattg aaatcgccat ctgggtggcg
     2641 gtgggcgggc gcggcacgct gattggcccg ctgctgggcg ccgggctgat caacctggcc
     2701 aagagctggt tcaccgtggc gtttcccgaa tactggctgt tttttctggg ctttttgttc
     2761 atcgccgtca cgctgttcct gccgcgtggg gtgatgggcc tgctcacccg caaaggagct
     2821 tccacatgag cccgcgcacc gacgccgcca ctggcgaaga ctttggctgg gaaggcgagg
     2881 ccaccggcct ggcccgcccg gtggacgcca gccagctgga cacccgccac ggcgatattt
     2941 tgtatctgga cgacatcacg gtgagttttg acggctttcg ggcgctgaac aaactcaccc
     3001 tggacatcgc cgtgggcgag ctgcgctgca tcatcggccc caatggcgcc ggcaaaacca
     3061 ccatgatgga cgtcatcacc ggcaaaaccc gcccagacgc cggcacggcg ttttttggcc
     3121 agaccatcga cctcacccgg ctgaacgaac cgaccatcgc cc
//
LOCUS       DAOPYX010000333         3150 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_42036_length_3150_cov_7.646204, whole genome shotgun sequence.
ACCESSION   DAOPYX010000333 DAOPYX010000000
VERSION     DAOPYX010000333.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 3150)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3150)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3150
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42036_length_3150_cov_7.646204"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..297
                     /locus_tag="PKN55_12980"
     CDS             <1..297
                     /locus_tag="PKN55_12980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011134085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HNX72206.1"
                     /translation="ALKNMLDWASREPDNALLAGKPVAIMGAGGGMGTSRAQYHLRQV
                     CVYLDLHPLNKPEVFANAFAGGFDEHGDVRDERLLGQLAAQMQALKDWTLRLRA"
     gene            complement(446..880)
                     /locus_tag="PKN55_12985"
     CDS             complement(446..880)
                     /locus_tag="PKN55_12985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018747811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNX72207.1"
                     /translation="MLDQNSRDPALDEALELFFYGFRAFTALPDQVLAERGLQRVHHR
                     ALYFVGRHPGLSVNALLGILGVSKQALNAPLRQLIERGLIAVEVGQHDRRVRELTLTP
                     AGAELEAALSAAQHALMGKVFAEVGPEAEGQWKRVMRALAAR"
     gene            955..2109
                     /locus_tag="PKN55_12990"
     CDS             955..2109
                     /locus_tag="PKN55_12990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012696547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HNX72208.1"
                     /translation="MLSQRTARLTSSLIRDILAAAQRPGMISFAGGLPASETLALPRL
                     EDVPDGVMQYGPSEGEPALREAVAERARAMGMRCDASQVLILTGSQQGIDLVSKLLID
                     PGTPVLLEAPTYLAALQSFQFFGADCRSVALGEHGPDLDALRAGLAAKPAFAYLIPTF
                     QNPSGRCYDAAHRDATAEALDAQQVLLVEDDPYRELVYDEACERTPLCARLTRAPWIY
                     LGSFSKTLAPGLRVGYLIASPELFPYLLKLKQAADLHSNRLSQWLVLSQLQGDFAGHL
                     ARLRTLYRERRDQMHAALSEQLGEAANWQCPPGGLFFWLQLNTPGIDTRTLLPEAMAR
                     GLAFMPGEPFFPNAPAAVSAMRLNFSHSDPASLRQGVRLLAELLHEQQEG"
     gene            2533..3045
                     /locus_tag="PKN55_12995"
     CDS             2533..3045
                     /locus_tag="PKN55_12995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX72209.1"
                     /translation="MRINPAYKKIIEETMERYGIQDHRLEMGGKHAHLLFSHDGRSHK
                     VTIPGSPSDSQRGLKNFACDLRRRIVGMNVNAASDMPAEEILPDAVLLEKIQLRLAEN
                     PPQHATERDFQALKLLDGRMRSAVAIGQDAHADNPAAWGVERLERLVWLGLAEGDGQG
                     RYRKMGGTRQ"
BASE COUNT          581 a          947 c          996 g          626 t
ORIGIN      
        1 gcgctgaaaa acatgctgga ttgggcttcg cgcgagccgg ataacgcctt gctggccggc
       61 aagccggtgg cgatcatggg ggcgggcggc ggcatgggca cgtcgcgggc gcaataccat
      121 ttgcgccagg tgtgcgtgta tctggatttg catccgctga acaagccgga ggtgttcgcc
      181 aatgcctttg ccggcggttt tgacgagcat ggcgatgtgc gcgacgagcg cttgctgggc
      241 cagttggcgg cgcagatgca ggcgctgaaa gactggactt tgcgtttgcg tgcatagcag
      301 gcggtgtggc cacacccgct catccgggtg tggtgcttgg cagcgtcact gggggctcgc
      361 gtcactgggc actggtgcgc agcagaggca gcagcgcaca ggcggcgagt ttctgaccat
      421 atcgcaaacc tctttgaacg tgtgctcagc gcgccgccaa cgcgcgcatc acccgcttcc
      481 attgcccctc ggcctcgggg ccgacttcgg caaacacctt gcccatcagc gcgtgctggg
      541 cggcggacaa tgccgcttcc agctcggcgc ccgccggggt gagcgtcagc tcgcgcaccc
      601 ggcggtcgtg ctggcccact tcgacggcaa tcagcccgcg ttcgatcaat tgccgcagcg
      661 gcgcgttcag cgcctgcttg ctcaccccga gaatgcccaa tagcgcattg acgctcagcc
      721 caggatggcg gccgacaaaa tacagcgcgc ggtggtgcac gcgttgcagg ccgcgttcgg
      781 ccagcacttg gtcgggcagg gcggtgaagg cgcgaaagcc ataaaaaaac agttccagcg
      841 cttcgtccag cgccgggtca cgggaatttt ggtcaagcat gttgactatt ttatccaaag
      901 tagcgaataa ttaggtcaac tttattgact aaagccaacc cacggagacc cgtcatgtta
      961 tcgcagcgca ccgcccgcct cacctcttcc ctgatccgcg acatcctcgc cgccgcccag
     1021 cggccgggca tgatttcgtt tgccggcggc ctgccggcca gcgaaacgct ggcgctgccc
     1081 cggctggaag atgtgccgga cggggtgatg caatacggcc cgtccgaagg tgagccggcg
     1141 ttgcgcgagg cggtggccga gcgggcgcgc gccatgggca tgcgctgtga cgccagccag
     1201 gtgctgatcc tcaccggctc gcagcagggc attgatctgg tgtccaagct gctgatcgac
     1261 cccggcacgc cggtgctgct ggaagcgccg acgtatctgg cggctttgca gtcgtttcag
     1321 ttttttggcg ccgattgccg cagcgtggcg ctgggcgagc acgggccgga cctggacgcg
     1381 ctgcgcgccg ggctggcggc caagccggcg tttgcctatt tgattccgac ttttcagaac
     1441 ccgtccggcc gctgctacga cgccgcccac cgcgatgcaa cggccgaggc gctggacgcc
     1501 cagcaggtgc tgctggtgga agacgacccc taccgcgagc tggtgtacga cgaggcctgc
     1561 gagcgcacgc cgctgtgcgc gcggttgacg cgcgcgccgt ggatttatct gggcagcttt
     1621 tcgaaaaccc tggcccccgg cctgcgcgtg ggctatctga ttgcttcgcc cgagttgttt
     1681 ccgtatctgc tcaagctcaa gcaggcggcg gatttgcaca gcaaccggct gagccagtgg
     1741 ctggtgctgt cgcaattgca gggcgacttt gccggccacc tggcccggct gcgcacgctg
     1801 taccgcgagc gccgcgacca gatgcacgcg gcgctgagcg agcaattggg tgaggcagcc
     1861 aactggcaat gcccgccggg cgggctgttt ttttggttgc agctgaacac gccgggcatc
     1921 gacacccgca cgctcttgcc cgaagcgatg gcgcgcgggc tggcctttat gccgggcgag
     1981 ccgtttttcc cgaatgcgcc ggcggcggtg agcgcgatgc ggctgaattt cagccacagc
     2041 gacccggcca gcctgcgcca gggggtgcgc ttgctggctg agttgctgca tgagcagcaa
     2101 gaggggtgaa cccggctgtg caggctaagc gggaattttc atgcgttaaa attatggatc
     2161 atgccaaata agttcaccct gtgcgcaagt gagcgaagtt catgaaaaaa tgcactaaac
     2221 cacgctgacg acagggaact tttgctgaca taatccggtc tttcggatta cccacactgc
     2281 tgacccacac cagcttgtca tcgcaagctt tcgctgtggg tcatgccttt ccggctcaca
     2341 actgagactg actttcgttc atgtcgatag ccagacttgc tatattcgtg cctttgtacg
     2401 tataaatact taaaatacgt gcttgaattt ttgcacgaac aagcggaaca tcacatctgg
     2461 ccgtcagcaa tagttgacta ctgtcagcaa ttgtcagagt gaccgtatgt tttacagaag
     2521 gcttacccta atatgcgcat caatcctgct tacaaaaaga tcattgaaga gaccatggaa
     2581 cgctatggca ttcaggatca ccgcctggaa atgggtggca agcatgctca cctgctgttc
     2641 agccacgatg gccgttcgca caaggtcacc ataccgggca gtccgtctga ttcgcagcgc
     2701 gggctgaaaa actttgcctg cgacctgcgc cgccggattg tcggcatgaa cgtcaacgcc
     2761 gccagtgata tgccggcaga agaaatattg cccgacgcag ttcttctgga aaaaatccag
     2821 ctgcgtctgg cggaaaaccc gccacaacat gccaccgagc gcgattttca ggcgctcaag
     2881 ctgctggacg gacgcatgcg ctcggccgtg gctatcggcc aggacgccca tgccgacaat
     2941 ccggccgcct ggggggtcga acgactggag cggctggtgt ggctgggcct ggccgaaggc
     3001 gatggtcagg gccgttaccg caagatgggc ggcacccgcc agtaagcttg tacgcctttg
     3061 gcgtgcgtgc agccttgaca tcgtgtcgct tttatatagt ttacggttgc gcaactgtca
     3121 gtatgattga cgacgtcagt ccggtttgct
//
LOCUS       DAOPYX010000334         3124 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_42550_length_3124_cov_7.696644, whole genome shotgun sequence.
ACCESSION   DAOPYX010000334 DAOPYX010000000
VERSION     DAOPYX010000334.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 3124)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3124)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3124
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42550_length_3124_cov_7.696644"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..908
                     /gene="arsB"
                     /locus_tag="PKN55_13000"
     CDS             <1..908
                     /gene="arsB"
                     /locus_tag="PKN55_13000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018747739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008490 - arsenite secondary active
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ACR3 family arsenite efflux transporter"
                     /protein_id="HNX72210.1"
                     /translation="LPVGVLIWVMIIPMLVKVDFAAVHQVRQHVRGIGVTLFVNWLVK
                     PFSMALLGWLFVRQLFAPWLPAEQLDSYIAGLILLAAAPCTAMVFVWSRLTQGDPLFT
                     LSQVALNDVIMVLAFAPLVGLLLGVSAIAVPWDTLLTSVLLYIVAPVLLAQLWRRALL
                     ARGQAAFDAAMARIGPWSMAALLLTLVLLFAFQGEAILRQPLVIALLAVPILLQVLFN
                     SALAYWLNRALGEQHCVAGPSALIGASNFFELAVAAAISLFGLHSGAALATVVGVLIE
                     VPVMLLVVRVVNGSKAWYEAGALRR"
     gene            996..2378
                     /locus_tag="PKN55_13005"
     CDS             996..2378
                     /locus_tag="PKN55_13005"
                     /inference="COORDINATES: protein motif:HMM:NF024700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNX72211.1"
                     /translation="MCLVHVQRFVWPHARPPTEEHVMWIERLHLSNYRCFEDFEIAFR
                     PGFNVIAGVNGSGKTSLLMGLCQAACPITLSSALKKPGNTVLGLLSEEGQARLKMEVF
                     NGRFRFEPQYPIAIMISASYADKKYNWSIEQQNAIARPSIHGQHFDATSDHQQTAGQP
                     ELLPIVVCYPANRQWRVPKNKRDNEAQAITERQSRFHGYVNWWNASADTNALQTWVIS
                     KCLERFQTSSETGLKFDDIDDDELALVNHALSAVLTEAKGLKYDLKQKSLLMEWRADS
                     GDARPATPFDQLSDGQRAMIALVADIARRMCLLNPQLGAHVTAATPGVVLIDELDMHL
                     HPHWQRLLTRGLKQAFPAVQFIVTSHSPQVLGELQAEEIILLQGAGTAHPQASYGLSS
                     SQILQEIMGAEARTGQVQTALDQLFAALEHNQLEQARVCLQQLRADAPDIPELAGAAA
                     LLRRKEVLGR"
     gene            2375..3028
                     /locus_tag="PKN55_13010"
     CDS             2375..3028
                     /locus_tag="PKN55_13010"
                     /inference="COORDINATES: protein motif:HMM:TIGR02646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02646 family protein"
                     /protein_id="HNX72212.1"
                     /translation="MKQVNKGASPAAFERWKALANDDWQPCYAELQNPEKTLLHTALL
                     QEQGHVCCYCGRQISLTDSHIEHWRPQALRKDLELDYSNLLASCLRASQPGAPLHCGH
                     AKGNAFIEEHTLSPLDPGCELRFAYHLNGAISAARPDDQAAIDMIALLQLDIAFLRNR
                     RSEALTRVFDAAFLGTASDDELRTLATVYRQLDPDSGQHADFGHVLALFAEALLPET"
BASE COUNT          610 a          945 c          941 g          628 t
ORIGIN      
        1 atctgccggt gggcgtgctg atctgggtga tgatcatccc gatgctggtc aaggtggatt
       61 ttgccgccgt gcaccaggtg cgccagcatg tgcgcggcat tggcgtgacc ttgttcgtca
      121 actggctggt caagccgttt tccatggcgc tgctgggctg gctgtttgtg cgccagctgt
      181 ttgccccgtg gctgccggcc gagcagctgg acagctatat cgccgggctg attctgctgg
      241 ccgccgcgcc gtgcaccgcc atggtgtttg tctggagccg gctcacccag ggcgacccgc
      301 tgttcaccct cagccaggtg gcgctgaacg acgtgatcat ggtgctggcc tttgccccgc
      361 tggtgggcct gctgctgggg gtgtcggcca ttgccgtgcc gtgggacacc ctgctgacct
      421 cggtgctgct gtatatcgtg gcgccggtgc tgctggccca gctgtggcga cgcgcgctgc
      481 tggcgcgcgg ccaggcggct tttgacgccg ccatggcgcg catcggcccg tggtcgatgg
      541 cggcgctgtt gctcaccctg gtgctgctgt ttgcttttca gggcgaggcg atcttgcgcc
      601 agccgctggt gattgccctg ctggccgtgc cgattctgct gcaagtgctg tttaactcgg
      661 cgctggccta ctggcttaac cgcgcgctgg gcgagcaaca ctgcgtggcc ggcccctcgg
      721 cgctgattgg cgcgtccaat ttctttgagc tggcggtggc cgccgccatc agcctgtttg
      781 gcctgcactc cggcgccgcg ctggccaccg tggtgggcgt gctgattgaa gtgccggtga
      841 tgctgctggt ggtgcgcgtg gtcaacggca gcaaggcgtg gtacgaggcg ggcgccttac
      901 ggcgctgagg gagagcaaag cgtaacagga atggaatgat gcgtgcgcgg ccacagtgct
      961 tttatacttg acccattggc gcaatgcgat gaggcatgtg ccttgtacat gtacaacgat
     1021 ttgtatggcc acacgcccgg ccgccgacag aggagcacgt aatgtggatt gaacggctgc
     1081 acctgagcaa ctaccgttgc ttcgaagatt tcgagattgc ctttcggccg ggcttcaatg
     1141 tcattgcggg ggtgaacggt agcggcaaaa cctcattgct catggggctt tgtcaggctg
     1201 catgcccgat aaccctttca agcgccttaa aaaagccagg caatacagta cttggccttt
     1261 tatctgaaga aggtcaggca cgcctcaaaa tggaggtgtt caatggcaga ttcagatttg
     1321 agccacaata ccccatcgca atcatgatat cggcttctta tgccgacaaa aaatataact
     1381 ggagcataga gcagcaaaac gctatcgctc gaccgagcat ccatggtcag cattttgatg
     1441 ccacatcaga tcatcaacaa accgcagggc aaccagaact tcttcctatc gtcgtatgtt
     1501 atccagccaa tcgccagtgg cgcgtgccga agaacaaaag agacaatgaa gcccaagcaa
     1561 tcaccgaacg acagtctcgc ttccatggtt atgtcaactg gtggaatgcg tccgcagata
     1621 ccaatgcgct acaaacctgg gtgatcagca aatgcctgga gcgctttcaa acttcgtcag
     1681 aaaccgggct gaaatttgac gacatcgacg atgacgagct ggcgctggtg aatcacgcct
     1741 tgtccgccgt gctgaccgaa gccaagggcc tgaaatacga cctcaagcaa aaaagcctgc
     1801 tgatggagtg gcgcgccgat agcggcgatg cccgccccgc cacgccattt gaccaactca
     1861 gcgatggcca gcgcgccatg attgccctag tggccgacat tgcccgccgc atgtgtttgc
     1921 tcaatccgca gttgggcgcg cacgtcaccg ccgccacgcc gggcgtggtg ctgatcgacg
     1981 agctggacat gcatctgcac ccacattggc aacggctgct cacccgtgga ctcaagcagg
     2041 cgtttccggc ggtgcagttt attgtcacca gccattcccc acaggtgctg ggtgaattgc
     2101 aagcagaaga gattatcttg ctgcaaggcg ctggcacagc gcacccgcaa gccagctacg
     2161 gcctgagctc cagccagatt ttgcaggaaa tcatgggcgc cgaggcgcgc accggccagg
     2221 tgcagaccgc actggatcaa ctgtttgccg cgctggagca taatcaactg gaacaagccc
     2281 gcgtatgcct gcaacaactg cgcgcagacg ccccggacat ccccgaactg gctggcgcgg
     2341 cggcgctgct gcgccgcaaa gaggtgctgg gccgatgaag caggtgaaca aaggcgcatc
     2401 acccgcagca tttgagcgct ggaaagcgct ggccaatgac gattggcagc cctgttacgc
     2461 cgaattgcaa aacccagaaa aaacccttct gcacaccgcc ttgctgcaag aacaaggcca
     2521 tgtgtgttgc tactgcggcc ggcaaatatc gctcacagac agccatatcg aacactggcg
     2581 cccgcaagca ttgcgcaaag acctggagct ggactacagc aatttgctgg cctcctgcct
     2641 gcgcgccagc caacccgggg ccccgctgca ttgtggccac gccaaaggca atgcttttat
     2701 cgaagaacac accctgtcgc cgcttgaccc aggctgcgag ctgcgctttg cttaccatct
     2761 taacggcgct atttctgcgg ccaggccgga tgatcaggcc gctatcgaca tgatcgccct
     2821 gctgcaactg gacattgctt ttctgcgcaa ccggcgcagc gaagcgctca cacgggtgtt
     2881 tgacgccgcg tttctgggca cagcaagcga cgacgagctg cgcaccctgg cgacggtcta
     2941 tcgccagctt gacccagaca gcggacaaca cgctgatttt ggccacgtcc tggccctttt
     3001 tgccgaggcg ctgctgccgg agacatagcc agccgactca ccccgccacg cgggcaaagc
     3061 gtcgtcgata ttccgctggc gcgcagtgca gttcgcgggt gaacagtttg cgaaacgcgc
     3121 cggt
//
LOCUS       DAOPYX010000335         3100 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_43057_length_3100_cov_8.506732, whole genome shotgun sequence.
ACCESSION   DAOPYX010000335 DAOPYX010000000
VERSION     DAOPYX010000335.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 3100)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3100)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3100
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43057_length_3100_cov_8.506732"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(133..1059)
                     /locus_tag="PKN55_13015"
     CDS             complement(133..1059)
                     /locus_tag="PKN55_13015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018747560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HNX72213.1"
                     /translation="MLKRVISLALTASLTTLPLMACATSANKAAPAAKASQSSGKVAS
                     NARVADHYDNGSAPALHSNAALVMNAQTGELIYAKNSNAVQPIASITKVMTAMVTLDG
                     KQSLDEMITITDDDVDKVKRSGSRLAVGVTLPRREMLLLALMSSENRAASALARTYPG
                     GTRAFVAKMNQKARSVGMRNAVFYDSTGLNSGNSATATDLARMVQAAYHYPLIRQFTT
                     TAEQDVWVSDSRRLHYRNSNALVREGEWQIGLSKTGYIKEAGRCLVMQATVNNLPLVI
                     VLLDGAGSQTRIQDARSIRSWLEQLPGSWLAG"
     gene            1162..>3100
                     /gene="uvrB"
                     /locus_tag="PKN55_13020"
     CDS             1162..>3100
                     /gene="uvrB"
                     /locus_tag="PKN55_13020"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HNX72214.1"
                     /translation="MFVTFPGSPYQLHQPFLPAGDQPAAIAKLVEGLEDGLAYQTLLG
                     VTGSGKTYTMANVIARTGRPAIIMAPNKTLAAQLYAEMREFFPENAVEYFVSYYDYYQ
                     PEAYVPSRDLFIEKDSSINEHIEQMRLSATKAILERPDCVIVATVSAIYGIGDPSAYH
                     QMILHLKEGEAKSQRDIISRLTLMQYDRNDMEFKRGVFRVRGDVIDIFPAESAELALR
                     VSLFDDEVETLQLFDPLTGQTRQRVGRYTVFPSSHYVTPRETVLRACEAIKAELNARR
                     QWFEQEGKLVEAQRLEQRTRFDLEMLYEMGFCKGIENYSRHFSGHAAGEAPPTLIDYL
                     PKNTLMIIDESHVTISQIGGMYKGDRARKENLVEYGFRLPSALDNRPLKFEEFEHKMP
                     QSIFVSATPADYEARHAGQVVEQLVRPTGLVDPEVEIRPVASQVDDLLSEITARVAKN
                     ERVLVTTLTKRMAEQLTDYYNEHGIKVRYLHSDIDTVERVEILRDLRLGVFDVLVGIN
                     LLREGLDIPEVSLVAILDADKEGFLRSERSLIQTIGRAARNLHGKAILYADRITGSME
                     RAIGETERRREKQLGFNAEHGIVPRGVQKRIKDIIDGVYSAESQARQGKAEALDEKQL
                     AKRIKETDKAMMDAARDLRFED"
BASE COUNT          640 a          940 c          959 g          561 t
ORIGIN      
        1 tttggccata cacacagaaa acggtaaaag aatattcgcg aagtgccgcc acacacccca
       61 gccaaaacac cgcgcgccgc cacctgagta caagtgagcg gcgcgcagag tttacgtgat
      121 gcagtgacgt gctcagccgg ccagccagct gcccggcagt tgttccagcc agctgcggat
      181 cgagcgggcg tcctggatgc gggtctggct gccggcgccg tccagcagca caatcaccag
      241 cggcaggtta tttaccgtgg cttgcatcac caggcagcgg ccggcttctt tgatatagcc
      301 ggttttcgac aggccaatct gccattcgcc ctcgcgcacc agcgcattgc tgttgcggta
      361 gtgcaggcgg cggctgtcac tcacccacac gtcttgctcg gcggtggtgg tgaactgacg
      421 gatcagcggg tagtgatacg ccgcctgcac catgcgcgcc agatcggtgg cggtggcgct
      481 gttgccgctg ttcagcccag tgctgtcgta aaacaccgca ttgcgcatgc cgacagaacg
      541 ggctttctgg ttcatcttgg caacaaaggc gcgggtgccg cccggatagg tgcgcgccag
      601 cgccgaggcg gcgcggtttt ctgaggacat cagcgccagc agcagcattt cacggcgcgg
      661 cagggtgacg cccaccgcca gacgcgaacc ggagcgtttg accttgtcca catcgtcgtc
      721 ggtgatggtg atcatttcgt ccagcgactg cttgccatcc agcgtcacca tggcggtcat
      781 caccttggtg atcgaggcaa ttggctgcac ggcattgctg ttcttggcgt agatcagctc
      841 gccggtctgc gcgttcatca ccagggcggc gttggagtgc agcgcggggg cagagccgtt
      901 gtcataatga tccgccacac gggcattgct ggcgactttg ccgctgctct ggctggcctt
      961 ggcggcgggg gcggctttgt tggcgctggt ggcgcaggcc atcagcggca gggtggtcag
     1021 gctggcagtc agcgccagcg agatgacacg tttcaacatg agtgggtgtt ccttacttgc
     1081 aagcagtgag attctacgca cgcgccggag gggaaacaat ctaaaatccc ggttttgcca
     1141 ttgctttgac gagtgtgcgc catgtttgtg acgtttccgg gcagtccgta tcaattgcac
     1201 cagccgtttt tgcccgctgg cgaccagccg gcggccatcg ccaagctggt ggaagggctg
     1261 gaagacggcc tggcttacca aaccctgttg ggggtcactg gttcaggcaa aacctatacc
     1321 atggccaatg tgatcgcccg caccgggcgg ccggccatca tcatggcgcc caacaaaacc
     1381 ctggccgcgc agctgtatgc cgaaatgcgc gagttttttc cggaaaacgc cgtcgagtat
     1441 tttgtctcgt actacgacta ctaccagcca gaggcttacg tgcccagccg ggatttgttt
     1501 attgaaaaag actcgtcgat caacgaacac atcgagcaga tgcggctgtc ggccaccaag
     1561 gcgattctgg aacggccgga ttgcgtgatt gtcgccaccg tgtcggccat ttacggcatt
     1621 ggcgacccca gcgcctacca tcagatgatc ctgcacctga aagaaggcga agccaagtcg
     1681 cagcgcgaca tcatcagccg gctgacactg atgcaatatg accgcaatga catggaattc
     1741 aagcgcgggg tgttccgcgt gcgcggcgat gtgatcgaca tctttccggc cgaatcggcc
     1801 gagctggcgc tgcgggtgag tttgtttgac gacgaagtgg aaaccctgca actgttcgac
     1861 ccgctcaccg ggcaaacccg ccagcgcgtg ggccgctaca cggtgtttcc gtccagccat
     1921 tacgtcaccc cgcgcgaaac cgtgctgcgc gcctgcgagg cgatcaaggc cgagctgaac
     1981 gcccgccgcc agtggtttga acaagaaggc aaattagtcg aggcgcagcg gctggagcag
     2041 cgcacccgtt ttgacctgga aatgctctac gagatgggct tttgcaaggg catcgaaaac
     2101 tactcgcggc atttttccgg gcacgccgct ggcgaagcgc cgccgacgct gattgattac
     2161 ctgcccaaaa acacgctgat gatcatcgac gaatcgcacg tcaccatcag ccagatcggc
     2221 ggcatgtaca agggcgaccg cgcgcgcaaa gaaaatctgg tggaatacgg ctttcgcctg
     2281 ccgtcggcgc tggacaaccg gccgctgaaa tttgaagaat tcgaacacaa aatgccgcaa
     2341 agcatttttg tctccgccac cccggccgac tacgaagccc gccacgccgg ccaggtggtc
     2401 gagcaactgg tgcgccccac cggcctggtg gacccggaag tggaaatccg cccggtggcc
     2461 agccaggtgg acgatttgct gtcggaaatc accgcgcggg tggccaagaa cgagcgggtg
     2521 ctggtcacca cgctgaccaa gcgcatggcc gagcagctga ccgactatta caacgaacac
     2581 ggcatcaagg tgcgctatct gcactcggac atcgacaccg tcgagcgggt ggaaatcctg
     2641 cgcgacctgc gcctgggcgt attcgatgtg ctggtgggca tcaacctgct gcgcgaaggg
     2701 ctggacatcc cggaagtcag cctggtggcg attctggacg ccgacaaaga aggctttttg
     2761 cgcagcgagc gcagcctgat ccaaaccatc ggccgcgccg cgcgcaattt gcacggcaag
     2821 gccatcctct acgccgaccg catcaccggc tcgatggagc gggccatcgg cgaaaccgag
     2881 cgccgccggg aaaaacaatt gggctttaac gccgaacacg gcatcgtgcc gcgcggcgtg
     2941 caaaagcgca tcaaggacat catcgacggc gtgtacagcg cagaaagcca ggcgcgccaa
     3001 ggcaaggctg aggcgctgga cgaaaaacaa ctggccaagc gcatcaagga aaccgacaaa
     3061 gccatgatgg acgccgcccg cgacctgcgc tttgaagacg
//
LOCUS       DAOPYX010000336         3070 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_43669_length_3070_cov_14.173466, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000336 DAOPYX010000000
VERSION     DAOPYX010000336.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 3070)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3070)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3070
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43669_length_3070_cov_14.173466"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..393
                     /locus_tag="PKN55_13025"
     CDS             <1..393
                     /locus_tag="PKN55_13025"
                     /inference="COORDINATES: protein motif:HMM:NF018627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX72215.1"
                     /translation="PPAWRDAVQAVIDAPGFYRQLPVMAGAQAALAALAARGYRLTVC
                     TSPGATLAERRAWLAEHFGPALAQGMLACADKTQVDGDWLIDDNPAPSGACAPRWRHI
                     VFDQPYNRHVPGLRLNWQNWAQHWPESG"
     gene            487..2073
                     /locus_tag="PKN55_13030"
     CDS             487..2073
                     /locus_tag="PKN55_13030"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNX72216.1"
                     /translation="MNLPDPPSLSARATELETAAAPLTAHWNPSLAAHVLYLALNLKC
                     MADSRQLPRLSEAAKGLVHFLHHRMNQGHHPADLHFADELRTRLNMIVQHAAPSVKVT
                     PIARASIGPVSLLALSGDDAPLARHLAHFGIPVERFTSLPELAEAASKQVPAAVIVLG
                     DPHAHDNQLSHALSALKHAMLPETVLLILSPDTGFEARLAAVRAGARGFLPWPCPFNE
                     VLDTLTPLLTRDDPVPLRVLLVEDLESLASFYASVLAERGIDTRVVSNPHTLLDTIIT
                     FRPDLILLDMALPGCSGMELAGVIRQQRYLDNIPLLFITSEKQRPRHLDAHDIGVDGF
                     LIKPVPADVLAMTVMARARRHRRLAGHITTDGLTGLLNHSQLLSQLDMELVRTRRQQE
                     VLSFVQLNIDHFKDINDTHGHPIGDQVLRTLARILRERLRRSDIVGRLGGDEFGVIMP
                     ATEPGQAKVLIEELAAAFHRVAHSANTATFWLSFRAGVAFTRDGGESRTLLAEAAAAV
                     QQAKAQGGGVVVADSVSPAA"
     gene            complement(2155..2946)
                     /locus_tag="PKN55_13035"
     CDS             complement(2155..2946)
                     /locus_tag="PKN55_13035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017507526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HNX72217.1"
                     /translation="MNRLPRHEQILKLIRANGFMPIEELARRLDVTPQTIRRDINQLS
                     EENLLRRFHGGAALGPSVENEEYSVRKVKHQAEKARIGELVASHIPDDASLFMNIGTT
                     IEAVARALCNTHKHLRIVTNCINVATVLTRRQDFEIIITGGVVRHKDGGITGVATLDM
                     IEQFRVDYAIVGASGVDLDGSLMDYDYREVRAAQAMMKNARQRYLAVDSSKFGRNAMM
                     KLCHISEVNDVFTDDDPPAELAPILADKNVRLHVARAGEPILPEE"
BASE COUNT          507 a          969 c         1005 g          589 t
ORIGIN      
        1 ccaccggcct ggcgcgacgc cgtgcaggcg gtgattgacg cgccggggtt ttatcgtcag
       61 ttgccggtga tggccggcgc acaagccgcg ctggcggcgt tggcggcgcg cggctatcgg
      121 ctgacggtgt gcacctcgcc cggcgccacg ctggccgagc ggcgcgcctg gctggccgaa
      181 cattttggcc cggcattggc tcaaggcatg ctcgcctgcg ccgacaaaac ccaggtggac
      241 ggcgattggc tgattgacga caacccggcg ccgtccggcg cgtgcgcgcc gcgctggcgg
      301 catatcgtat ttgaccagcc ctataatcgc cacgtccccg gcctgcggct gaactggcaa
      361 aactgggcgc agcactggcc cgaatcgggc tgagtgtggc cggatgatgc agtgcaaaag
      421 ctggtgtttg cgcgcgtgcg ctgtttaaat gaaagcgtct gcctcatttg ctgcaaaggc
      481 ccggccatga acctgcccga tcccccctct ttgtcggcgc gcgccaccga actggaaacc
      541 gccgccgccc cgctgaccgc gcactggaac ccctcgctgg ccgcccatgt gctgtatctg
      601 gcgctgaatc tcaagtgcat ggccgactcg cgccagctgc ctcgcctctc tgaagcggcc
      661 aaggggctgg tgcattttct gcatcaccgc atgaaccagg gccatcaccc cgccgacctg
      721 cattttgccg acgagctgcg cacccggctg aacatgattg ttcagcacgc cgccccatcg
      781 gtgaaagtca ccccgattgc ccgcgccagc atcggcccgg tcagcctgct ggccctgagc
      841 ggcgacgatg cgccgctggc gcgccatctg gcgcactttg gcatccctgt cgagcgcttc
      901 accagcctgc ctgagctggc cgaggcagcc agcaagcagg tgccggcggc ggtgattgtg
      961 ctgggcgacc cacacgccca cgataatcag ctctcgcatg cgctgtcggc gctcaagcac
     1021 gccatgctgc cagaaaccgt gctgctgatc ctgtcgccgg acaccggctt tgaggcgcgc
     1081 ctggcggcgg tacgcgctgg cgcgcgcggc tttttgccgt ggccctgccc gtttaatgaa
     1141 gtgctggaca cgctgacccc gctgctgacc cgcgatgacc cggtgccctt gcgcgtgctg
     1201 ctggtggaag acctggaatc actggccagt ttttacgcct cggtgctggc cgagcgcggc
     1261 atcgacaccc gcgtggtgag caatccgcat accctgctgg acaccatcat caccttccgg
     1321 ccggatttga ttctgctgga catggcgctg ccgggctgct ccggcatgga gctggccggg
     1381 gtgatccgcc agcaacgcta tctggacaat atcccgctgc tgttcatcac cagcgaaaaa
     1441 cagcggccgc gccatctgga cgcccacgat atcggcgtgg atggcttttt gatcaaacca
     1501 gtgccggccg atgtgctggc gatgacggtg atggcgcgcg cgcgccgtca ccgccggctg
     1561 gctggccata tcaccaccga tgggctgacc ggcttgctga atcattcaca attgctgtcg
     1621 cagctggaca tggagctggt gcgcacccgc cgccagcagg aagtcttgtc gtttgtgcag
     1681 ctgaatatcg accacttcaa ggacatcaac gacacccacg gccacccgat tggcgaccag
     1741 gtgctgcgca cgctggcgcg catcctgcgc gagcggctgc gccgcagcga catcgtcggc
     1801 cggctgggcg gcgatgaatt tggcgtgatc atgccggcca ctgagcccgg tcaggccaag
     1861 gtgttgatcg aagaactggc cgccgcgttt catcgggtgg cgcacagcgc caacacggcg
     1921 acgttctggc tgagttttcg ggctggcgtg gcctttaccc gcgatggcgg cgaatcgcgc
     1981 accctgctgg ccgaggcggc agcagcagtg cagcaggcca aggcgcaggg cggcggcgtg
     2041 gtggtggccg atagcgtgtc gcccgcggca taaaacacac acccacaata caacgccccg
     2101 ccggaatgct gttcctggcg gggcgttgta ttgtggggcg tctgttcgct gctcttactc
     2161 ttccggcaaa atcggctcgc ctgcgcgcgc cacatgcagg cgcacatttt tatcggccag
     2221 aatcggcgcc agctcggctg gcgggtcgtc gtcggtgaac acatcgttca cttcgctgat
     2281 gtggcacagc ttcatcatgg cgttgcggcc gaacttgctg gagtccactg ccagatagcg
     2341 ttggcgggcg tttttcatca tcgcctgcgc tgcgcgcact tcgcggtagt cgtagtccat
     2401 cagcgagcca tccaggtcca cgcccgaggc gccgacgatg gcgtaatcca cccgaaactg
     2461 ctcgatcatg tccagcgtgg ccacgccagt gatgccgccg tctttgtggc gcaccacgcc
     2521 gccggtgatg atgatttcaa agtcttgccg gcgggtgagc acggtggcga cgttgatgca
     2581 gttggtgacg atgcgcaggt gtttgtgggt gttgcacagc gcgcgcgcca cggcctcgat
     2641 ggtggtgccg atgttcatga acagcgaggc gtcatcgggg atgtggctgg ccaccagttc
     2701 gccgatgcgg gctttttcgg cctggtgttt gaccttgcgc accgagtatt cctcgttttc
     2761 cacgctgggg ccaagtgcag cgccgccgtg aaagcgacgc agcaggtttt cttccgacag
     2821 ttggttgatg tcacggcgga tggtttgcgg ggtgacgtcc agacggcggg cgagctcttc
     2881 gatgggcatg aagccgttgg cgcggatcag cttgaggatt tgctcgtgac ggggcaggcg
     2941 gttcatggga aaagaaaaat gggtcgggaa agaccgattg gttttagcac gaaaaacacc
     3001 agtggcgcaa atcagggcgg gcgagatggc gcaaaactgc aactggggtt ttcagatatg
     3061 aacaagttgg
//
LOCUS       DAOPYX010000337         3043 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_44246_length_3043_cov_11.198795, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000337 DAOPYX010000000
VERSION     DAOPYX010000337.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 3043)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3043)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3043
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44246_length_3043_cov_11.198795"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..548)
                     /locus_tag="PKN55_13040"
     CDS             complement(<1..548)
                     /locus_tag="PKN55_13040"
                     /inference="COORDINATES: protein motif:HMM:NF014671.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HNX72218.1"
                     /translation="MQREFFASPDTHPQTAAHVPVRRLCADGWQRDAVDVLVEETPVA
                     LVYNGISHAVMLATPSELDDLALGFSLSEGIVQDPSEVYALDYEHGPDGVSVHLDIAS
                     RRFHALKERRRALAGRTGCGLCGVDSLDGVARCHSRVARGAPLLASAIFAAVKQLDRH
                     QPLRQSTGAAHAAAWVNLAGDI"
     gene            complement(600..1220)
                     /locus_tag="PKN55_13045"
     CDS             complement(600..1220)
                     /locus_tag="PKN55_13045"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011137388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit gamma"
                     /protein_id="HNX72219.1"
                     /translation="MKEQLIQRYSAPERINHWVVAVCFVLLAISGLAFFYPAFFWLTG
                     VFGTPQLARIVHPFVGVVMFLAFARQFFRYWHHNLITPEDVKWMKSVKEVMKGNEVGD
                     IGKYNGGQKGMFWLMCACMAVLMVSGLIAWRQYFAGYFPIPVVRLALLAHASAAVALI
                     AGIIVHVYAALWVKGTIRAMVEGVVTHAWAKKHHPRWYREMTGKKS"
     gene            complement(1217..2089)
                     /gene="fdxH"
                     /locus_tag="PKN55_13050"
     CDS             complement(1217..2089)
                     /gene="fdxH"
                     /locus_tag="PKN55_13050"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011137387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0045333 - cellular respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit beta"
                     /protein_id="HNX72220.1"
                     /translation="MALQSLDILRRSATPTATPQARTPVEVAKLIDVSTCIGCKACQV
                     ACSEWNDIREEVGSCVGVYDNPVDLTAQAWTVMRFAETEENGKLEWLIRKDGCMHCAD
                     PGCLKACPSPGAIIQYKNGIVDFHQENCIGCGYCVAGCPFNVPRLSPADNKAYKCSLC
                     SDRVAVGQEPACVKTCPTGAIQFGSKEDMKEVASVRIADLKNRGYQNAGLYDPEGVGG
                     THVMYVLHHADKPELYSNLPKDPAISTTVSLWKGLLKPLATLGIAAAGLFGFFHYITV
                     GPNRPDEDHEETRK"
     gene            complement(2103..>3043)
                     /locus_tag="PKN55_13055"
     CDS             complement(2103..>3043)
                     /locus_tag="PKN55_13055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011137386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HNX72221.1"
                     /translation="FDPKDPTKQLAKKGEQLPGFALLRDDGSTMSACWIFSGCWTQAG
                     NQMSRRDNTDVGLGNTPGWAWAWPANRRILYNRASCTPEGKPWDPSRKLIAWNGEKWA
                     GIDVPDFKADAGPDTGMNPFIMNPEGVGRLFAVDKLADGPFPEHYEPMESPIGTNPLH
                     PKVVSSPAVRIFKGDRERLGTHKEFPYVGTTYRLTEHFQFWTKSVRLNAIAQPEQFVE
                     ISEQLAKEKGIAQGDWVKVSSKRGFIKAKAVVTKRVKPLTVNNQTVHQIGIPLHWGWE
                     GVAKKGFLTNVLPPFVGDCNTQTPEYKSFLVNIEKA"
BASE COUNT          503 a          945 c          999 g          596 t
ORIGIN      
        1 tcgatgtcgc cggccagatt cacccaggcg gcggcgtgcg cggcgccggt gctctggcgc
       61 agcggctgat ggcgatccag ctgtttgacg gcggcaaaga tggccgaggc cagcagcggc
      121 gcgccgcgcg ccacccggct gtggcagcgc gccacgccat ccaggctgtc cacgccgcac
      181 aggccgcagc cggtgcgccc ggccagcgcg cggcggcgtt ctttcagcgc atgaaagcgc
      241 cggctggcga tgtccagatg gacgctgacg ccatccgggc cgtgttcata gtccagtgcg
      301 tacacttcgg acgggtcttg cacaatgcct tcgctcaggc tgaagcccag cgccaggtcg
      361 tccagctcgc tgggggtggc cagcatcacc gcgtgggaga tgccgttgta caccagcgcc
      421 accggggttt cttccaccag cacatccacc gcgtcgcgtt gccagccgtc ggcgcacagg
      481 cggcgcaccg gcacatgggc ggcggtttgc ggatgggtgt ctggagaagc aaaaaattcg
      541 cgttgcatgt cgggctcccg cccgtccggc gggcgcaagg ccagccggac gggggtggat
      601 caggacttct tgccggtcat ttcacgatac cagcgcggat ggtgtttttt tgcccaggca
      661 tgggtgacca cgccttccac catggcgcgg atggtgcctt tcacccacag cgcggcgtag
      721 acgtggacga tgatgccggc aatcagcgcc acggcggcgc tggcgtgcgc cagcaaggcc
      781 aggcgcacca ccggaatcgg gaaatacccg gcaaagtact gccgccaggc aatcaggccg
      841 gacaccatca gcaccgccat gcaggcgcac atcagccaga acatgccttt ttgcccgccg
      901 ttgtacttgc cgatgtcgcc gacttcgttg cccttcatca cttctttgac tgatttcatc
      961 catttcacgt cctcgggcgt gatcaggttg tgatgccagt agcggaagaa ctgtcgggca
     1021 aaggccagga acatcaccac gccgacaaac gggtggacaa tgcgcgccag ctgcggcgtg
     1081 ccaaacacgc cggtcagcca gaagaacgcc gggtaaaaga aggccaggcc agaaatcgcc
     1141 agcagcacaa agcacacggc gaccacccag tgattgatgc gctccggggc ggagtagcgt
     1201 tggatcagtt gctctttcac ttgcgggtct cctcgtggtc ttcatccggg cggttggggc
     1261 cgacggtgat gtagtggaag aagccaaaca ggccggcggc ggcaatgccc agggtggcca
     1321 gcggcttgag caggcctttc cacaggctga ccgtggtgct gatggccggg tctttcggca
     1381 gattgctgta cagctcgggc ttgtcggcgt ggtgcagcac atacatcaca tgggtgccgc
     1441 ccacgccttc cgggtcgtac aggccggcgt tttggtaacc gcggtttttc aggtcggcga
     1501 tgcgcacgga ggcgacttct ttcatgtcct ctttgctgcc gaactgaatc gccccggtcg
     1561 ggcaggtttt cacgcaggcc ggctcctggc ccaccgccac gcggtcggag cacaggctgc
     1621 acttataggc cttgttgtcg gccgggctca ggcgcggcac attgaacggg cagccggcca
     1681 cgcagtagcc gcagccgatg cagttttcct ggtggaaatc gacaatgccg tttttgtact
     1741 gaatgatggc gcccggcgac ggacaggctt tcaggcagcc ggggtcggcg cagtgcatgc
     1801 agccgtcctt gcggatcagc cattccagct tgccgttttc ttcggtttcg gcaaagcgca
     1861 tcaccgtcca ggcctgggcg gtgaggtcta ccgggttgtc gtagacgccg acgcaggagc
     1921 cgacttcctc gcggatgtcg ttccattcgg agcaggccac ctgacaggcc ttgcagccga
     1981 tgcaggtgga cacgtcaatc agcttggcca cttccaccgg ggttcgcgct tgcggggtgg
     2041 cggtcggggt ggccgagcgg cgcagaatat caagagattg cagagccatg gttatgcgcg
     2101 cctcatgcct tttcgatgtt caccaggaac gatttatatt caggcgtttg cgtattgcag
     2161 tcacccacga acggcggcag gacattggtc aagaagccct ttttcgccac gccttcccag
     2221 ccccagtgca gcggaatgcc gatctggtgc acggtctggt tgttcaccgt cagcggcttg
     2281 actcgcttgg tcaccaccgc cttggccttg atgaagccgc gcttggagct gaccttcacc
     2341 cagtcgccct gggcgatgcc cttttccttg gccagttgtt cgctgatttc gacaaactgc
     2401 tcgggttggg cgatggcgtt cagccgcacc gatttggtcc agaactggaa gtgctcggtc
     2461 agccggtagg tggtgcccac atacgggaat tctttgtgcg tgcccaggcg ctcgcggtcg
     2521 cccttgaaga tgcgcaccgc cgggctgctg accactttcg ggtgcagcgg gttggtgcca
     2581 atcgggcttt ccatcggctc gtagtgctcg gggaaggggc cgtccgccag tttgtccacg
     2641 gcgaacaggc ggcccacgcc ttcagggttc atgatgaacg ggttcatgcc agtgtccggg
     2701 ccggcgtcgg ccttgaagtc gggcacgtcg atgccggccc acttttcgcc attccaggca
     2761 atcagcttgc ggctcggatc ccacggcttg ccttctggcg tgcacgaggc gcggttgtac
     2821 agaatgcggc gattggccgg ccaggcccac gcccagccgg gggtgttgcc caggccgaca
     2881 tcggtattgt cgcggcggga catctggttg ccggcttgcg tccagcagcc cgagaaaatc
     2941 cagcaggcgc tcatggtgct gccgtcgtcg cgcagcaggg caaagccggg cagttgctcg
     3001 ccttttttgg ccagttgctt ggtcgggtct ttcgggtcga aca
//
LOCUS       DAOPYX010000338         3039 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_44323_length_3039_cov_8.870979, whole genome shotgun sequence.
ACCESSION   DAOPYX010000338 DAOPYX010000000
VERSION     DAOPYX010000338.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 3039)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3039)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3039
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44323_length_3039_cov_8.870979"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..334)
                     /locus_tag="PKN55_13060"
     CDS             complement(<1..334)
                     /locus_tag="PKN55_13060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012697904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase-peroxidase"
                     /protein_id="HNX72222.1"
                     /translation="MEHAIEQGTGGCPFAHGGQTSAGMAQPWWPKALNLDILDQHDSK
                     TNPLGAGFNYREALKTLDVDALKADLKALMTDSQPWWPADWGHYGGLMIRMAWHSAGT
                     YRIADGRGG"
     gene            802..>1134
                     /locus_tag="PKN55_13065"
     CDS             802..>1134
                     /locus_tag="PKN55_13065"
                     /inference="COORDINATES: protein motif:HMM:NF019237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1566 domain-containing protein"
                     /protein_id="HNX72223.1"
                     /translation="MGSYTIVDTNQTSYYGNTTTISTPASNASFYGQDAGYQGKQPSY
                     VDNGNSTVTDLNTGLTWMKSTTSQEMTWAQAVSYASTAVIGGYSDWRLPTIKELYSLI
                     EFSGYTGTS"
     assembly_gap    1135..1234
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1235..2434
                     /locus_tag="PKN55_13070"
     CDS             <1235..2434
                     /locus_tag="PKN55_13070"
                     /inference="COORDINATES: protein motif:HMM:NF019237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4214 domain-containing protein"
                     /protein_id="HNX72224.1"
                     /translation="VRGNTSYGQNAYVDNGNGTITDNATGLMWLQADSGSAMSWQDAL
                     AYAEASTASGYSDWRLPNAKELQSIVDYTRSPDTTGTAAIDPLFKATNIGTSSAPEYG
                     FYWTGTSHVENGSGDYAVYVAFGRALGWMQQKDGSYKLMDVHGAGAQRSDPKTGNASD
                     YPHGFGPQGDVIRINNMVRLVRDASSTSSDNTNQSFTGTSGNDSFTGGTGNDTIDGGA
                     GIDTAVFSNKIADYTRSKSGSVWTIKANTGTDGTDTVSNVERLHFSDGNVALDTDGAA
                     GQAYRLYRAAFAREPDSGGVGYWMAQMDKGMSLATAASSFIASSEFQARYGSAPSNGD
                     LLTKLYSNVLGRAADQSGYDWWLTQMNNGLSKTNVLVEFAQSAENQSAVATLIGSTGF
                     AYTEWLG"
     gene            complement(2598..2936)
                     /locus_tag="PKN55_13075"
     CDS             complement(2598..2936)
                     /locus_tag="PKN55_13075"
                     /inference="COORDINATES: protein motif:HMM:NF017355.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system HicB family antitoxin"
                     /protein_id="HNX72225.1"
                     /translation="MSLDHYTYRVTWSPEDGEHVGLCIEFPSLSWLAETPEEALAGVR
                     KVVAEVVADLEANGELIPQPLAEKHYSGEFRVRIPPELHRQLALMAAEQGVSLNRLAS
                     AKLAGQVLSR"
BASE COUNT          620 a          938 c          854 g          527 t
ORIGIN      
        1 cgccgccacg gccgtcggcg atgcggtagg tgccggccga gtgccaggcc atgcggatca
       61 tcaggccgcc gtagtggccc cagtcggccg gccaccacgg ctggctgtcg gtcatcaggg
      121 ccttgaggtc ggctttcagc gcgtccacgt ccagggtttt cagcgcctcg cggtagttaa
      181 agccggcgcc cagcggattg gttttgctgt cgtgctggtc gaggatgtcc agattcagcg
      241 ccttgggcca ccagggctgg gccatgccgg cagaggtctg gccgccatgg gcaaacgggc
      301 agccgcccgt tccttgttcg attgcgtgtt ccattgtgcc tccttcgtgg ggtgtggggt
      361 gcgccgccgg gtgaggcgga cgccatgcgc tgacgacctg gctgtgttct caccttagca
      421 ggcggcatgg cgtttgcctg tgcttgggcg acatcagcgt gtggcagcag atgaatgtag
      481 tgtgcgccag cgatggcgat taatctaatt aaacgttttt cttgtgtcga ttgcttaaag
      541 ctatacaccc accccggcaa atcatgaaca aggaataaaa ttctggctta gccattcatt
      601 cttcactcga ttgtccgatg aaaagccatt gcgcccggca tacgcgcggc tcacacaact
      661 cagtgcagca agcctggcca gacgcacaag cgcagaccgt gcgcgttgta caacaaggtc
      721 gggcaacaac gccaagcgcg tgtgtgagcg acgctcagct cgggcagaga acccgcagga
      781 cagaaaacag gagcgcagcc catgggcagc tacaccattg ttgacaccaa ccagaccagc
      841 tattacggca acaccaccac tatttccacc ccggccagca atgccagctt ttatggccag
      901 gatgccggtt atcagggcaa gcagccgtcc tacgtggaca acggcaacag cacggtcacc
      961 gacctgaaca ccggcttgac ctggatgaaa agcaccacca gccaggaaat gacctgggcg
     1021 caggcagtca gctatgcttc cacggcggtg attggcggct atagcgactg gcggttgccc
     1081 accatcaaag agctgtattc gctgattgag ttttctggct acaccggcac cagcnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnngtgcgc ggcaacacca gctatggcca
     1261 gaacgcctat gtggacaacg gcaatggcac catcaccgac aacgccaccg gcctgatgtg
     1321 gttgcaagcc gacagcggct cggcgatgag ctggcaggat gcgctggcct acgccgaagc
     1381 ctccaccgcc agcggctaca gcgactggcg gctgcccaat gccaaagagc tgcaaagcat
     1441 tgttgattac acccgctcgc ccgacaccac cggcacagcc gccatcgacc ccttgttcaa
     1501 ggccaccaat atcggcacat ccagcgcgcc ggaatatggc ttttactgga caggcaccag
     1561 ccatgtggaa aacggctcgg gcgactacgc cgtgtacgtg gcgtttggcc gcgcgctggg
     1621 ctggatgcag caaaaagacg gcagctacaa gctgatggac gtgcacggcg ctggcgccca
     1681 gcgcagcgac ccgaaaaccg gcaacgccag cgactacccg catggctttg gcccgcaagg
     1741 cgacgtgatc cgcatcaaca atatggtgcg gctggtgcgc gacgccagca gcaccagcag
     1801 cgacaacacc aaccaaagct ttaccggcac cagcggcaac gacagcttta ccggcggcac
     1861 cggcaacgac accattgatg gcggcgccgg catcgacacc gccgtgttca gcaataaaat
     1921 cgccgactac acccgcagca agagcggcag cgtgtggacc atcaaggcca acaccggtac
     1981 cgacggcacc gacactgtaa gcaatgtcga gcgcctgcac ttcagtgatg gcaatgtggc
     2041 gctggacacc gacggcgccg ccggccaggc ctaccgcctg taccgcgctg cctttgcccg
     2101 cgagccggac agcggcggcg tgggttactg gatggcacaa atggacaaag gcatgagcct
     2161 ggccaccgcc gcatccagct ttatcgcctc cagcgagttc caggcccgct acggcagcgc
     2221 ccccagcaat ggcgacctgc tgaccaagct gtattcgaat gtattgggac gcgccgcaga
     2281 tcaaagcggc tacgactggt ggctgacgca gatgaacaac ggcctgagca aaaccaatgt
     2341 gctggtggaa ttcgcccaat ccgccgaaaa ccagagcgcc gtggccacgc tgattggcag
     2401 cacgggcttt gcgtataccg agtggctggg ttaaccgccc ccgtcctctc gctgcgcgaa
     2461 ccggccttga aaccagaaag ggcggggtga tagagcgtct ggccgccgct agcagagccg
     2521 ctacactgag tttggtgagc ggctctaacg tgggctgtcc ggtattcttt tcttgtcttg
     2581 cagactgtcg gggtcgatca cctcgacagc acctgcccgg ccaattttgc actcgccaag
     2641 cgattaagac ttaccccttg ctccgccgcc atcagcgcga gctgacggtg cagttcgggt
     2701 ggaatacgca cgcgaaactc cccgctgtag tgcttctctg ccaaaggctg tgggatcaac
     2761 tctccattcg cctccagatc agcaaccacc tcggcaacca ccttgcgcac acctgccagg
     2821 gcctcttctg gcgtttctgc cagccagctt agcgagggaa actcgatgca caacccaaca
     2881 tgctcaccat cctcggggga ccatgttacg cgataagtat aatgatcaag actcattgcc
     2941 tgccctccag tttgtctatt gcttgcagca cctgccgcac ttgataaggc ttggccttac
     3001 caccagcgtc ctgaatattg actcgcgggt cccctggcc
//
LOCUS       DAOPYX010000339         3019 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_44760_length_3019_cov_10.102901, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000339 DAOPYX010000000
VERSION     DAOPYX010000339.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 3019)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3019)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3019
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44760_length_3019_cov_10.102901"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..1524)
                     /locus_tag="PKN55_13080"
     CDS             complement(1..1524)
                     /locus_tag="PKN55_13080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018749023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HNX72226.1"
                     /translation="MATPISTGSEWTFELIETYDRAIHDIAVGEYGLDIYPNQLEIIT
                     AEQMMDAYSSVGMPVNYHHWSFGKQFVSTEKSYKRGQMGLAYEIVINSNPCISYLMEE
                     NSMTMQALVIAHAAYGHNSFFKGNYLFRTWTDASAIIDYLVFARDYLSQCEERYGFDA
                     VEMLLDSCHALMNYGVDRYKRPAKLSLAQEKLRQREREAHQQLQINDLWRTIPRRQAS
                     GEDSEPARFPVEPQENLLYFIEKSAPLLEPWQREVVRIVRKVAQYFYPQRQTQVMNEG
                     WATFWHYTLLNRLYEKGLCTDGFMVEFLQSHTNVVYQPPVTSRWYSGINPYALGFAMY
                     QDIRRICQAPTAEDREWFPDIAGRPWLPTLDFAMRNFKDESFISQYLSPKLMRDFRLF
                     AVRDDDREDKLYVPAIHDESGYRQVRQTLAEQYNLGSREPNIQVWSVDVRGDRSLTLR
                     HTQHNRRPLEPNSADEVLKHIGRLWGFDVRLESVDDHGQVSKTFECKVDKAHRELLI"
     gene            complement(1560..2843)
                     /locus_tag="PKN55_13085"
     CDS             complement(1560..2843)
                     /locus_tag="PKN55_13085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011135342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaH/YhbH family protein"
                     /protein_id="HNX72227.1"
                     /translation="MSHLIDRRLNGKNKSAVNRERFLRRFRAQIRQSVARAIKGRSIT
                     DISSGEKVSIPVRDVSEPTFHHGSGGTREVVHPGNEEFVTGDRIARPDGGGGAGRGGK
                     ASDQGGGEDDFAFELSREEFMNFFFEDLALPNLIKTQLTGNSEWKTVRAGYTSDGTPT
                     NISIVRSLRGAQARRIALGAPSLARLHEAEEELDELLERCDEDDPQVRELRREIHQLR
                     EKASNIPFLDPFDLRYNNRARVPKPTSQAVMFCIMDVSGSMDEAKKDIAKRFFILLYL
                     FLQKSYDKIELVFIRHHTSAMEVDEENFFHSRESGGTVVSSALVLMRDIIQQRYPTDE
                     WNIYAAQASDGDNWDSDSANCGQLLDTQLLPLVQYFAYIEITEGEPQNLWYEYLRVQA
                     GHPQFAMQKIRNVADIYPVFRELFKRQPSTGREPA"
BASE COUNT          528 a          894 c          958 g          639 t
ORIGIN      
        1 tcagataagc agctcgcggt gcgccttatc caccttgcac tcaaaggttt tgctcacctg
       61 gccgtggtcg tccacgcttt ccagccgcac atcaaagccc cacagccggc cgatatgctt
      121 gagcacttcg tcggcgctat tgggttccag cggccggcgg ttgtgctggg tgtggcgcag
      181 ggtcaggctg cggtcgccgc gcacgtccac cgaccacacc tggatattcg gctcgcgcga
      241 gcccaggttg tactgctcgg ccagcgtttg ccgcacctgg cgatagccgc tttcgtcgtg
      301 aatcgccggc acatagagtt tgtcttcgcg gtcgtcgtcg cgcacggcaa acaagcggaa
      361 gtcgcgcatc agcttgggcg acaggtattg cgagataaag ctctcgtcct tgaagttgcg
      421 catggcaaag tccagcgtcg gcaaccaggg ccggccggca atatccggaa accattcgcg
      481 gtcttcggcg gtgggggctt ggcagatgcg gcggatgtcc tgatacatgg caaagcccag
      541 tgcatagggg ttgatgccgg agtaccagcg gctggtcacg ggcggctggt acaccacatt
      601 ggtgtgcgac tgcaaaaact ccaccatgaa gccgtcggtg cacaggcctt tttcgtatag
      661 ccggttcagc agggtgtaat gccagaaggt ggcccagcct tcgttcatca cctgggtttg
      721 ccgctgcgga taaaaatact gcgccacctt gcgcacgatg cgcaccacct cgcgttgcca
      781 gggctccagc agcggggcgg atttttcgat gaagtacagc aggttttctt gtggttccac
      841 cggaaaacgc gccggttcgc tgtcttcgcc gctggcctgg cggcgcggaa tggtgcgcca
      901 caggtcgttg atttgcagtt gctgatgcgc ctcgcgctcg cgctggcgca gtttttcctg
      961 cgccagcgac agcttggccg ggcgcttgta gcggtccacg ccgtagttca tcagcgcatg
     1021 gcaagaatcc agcagcattt ccaccgcatc aaagccataa cgctcttcgc attggctgag
     1081 gtagtcgcgg gcaaacacca ggtaatcaat gatggccgag gcgtcggtcc aggtgcggaa
     1141 caggtaattg cccttgaaaa aactgttgtg gccataggcg gcgtgggcaa tcaccagcgc
     1201 ctgcatggtc atcgagtttt cttccatcag atacgaaatg cacggattgg agttgatcac
     1261 aatctcgtaa gccaggccca tctggccgcg cttgtagctt ttttcggtgg agacaaactg
     1321 cttgccaaag ctccagtggt gatagttcac cggcatgccg accgacgaat aggcgtccat
     1381 catctgctcg gcggtgatga tttccagctg gttggggtag atgtccagcc catattcgcc
     1441 cacggcgatg tcgtgaatgg cccggtcgta ggtttcgatc agctcgaaag tccattccga
     1501 gccggtggag atcggggtgg ccatgatgca tcctccttga gggtcgccgc gcctggcgtt
     1561 caggcgggtt cgcggcccgt gctgggctga cgtttgaaca actcgcggaa caccgggtag
     1621 atatccgcca cattgcggat tttctgcatg gcgaactgcg gatggccggc ctgcacgcgc
     1681 aggtattcgt accacaggtt ttgcggctcg ccctcggtga tttcgatata ggcgaagtac
     1741 tgcaccagcg gcagcagctg ggtgtccagc agttggccgc aattggccga atcgctgtcc
     1801 cagttgtcgc cgtccgaggc ttgcgccgcg tagatattcc attcatcggt ggggtagcgc
     1861 tgctggatga tgtcgcgcat cagcaccagc gccgacgaca ccaccgtgcc gcccgactcg
     1921 cgcgagtgaa agaagttttc ttcgtccacc tccatggcgc tggtgtggtg gcggatgaac
     1981 accagctcga tcttgtcgta gctcttttgc aagaacagat acagcagaat gaaaaagcgc
     2041 ttggcgatgt cttttttggc ttcgtccatc gagccggaca cgtccatgat gcagaacatc
     2101 accgcctggc tggtgggctt gggcacgcgc gcgcggttgt tgtagcgcag gtcgaatggg
     2161 tccagaaacg ggatattgct ggctttttcg cgcagttgat gaatttcgcg ccgcagttcg
     2221 cgcacttgcg ggtcgtcttc gtcgcagcgc tccagcagtt cgtccagctc ttcttccgcc
     2281 tcgtgcagcc gcgccaggct gggcgcgccc agggcgatgc gccgcgcttg cgcgccgcgc
     2341 agcgagcgca cgatgctgat attggtgggc gtgccgtcgc tggtgtagcc ggcgcgcacg
     2401 gttttccatt cgctattgcc ggtcagctgg gttttgatca gattgggcag cgccaggtct
     2461 tcaaaaaaga agttcatgaa ctcttcgcgg ctgagttcaa aggcaaagtc atcctcgccg
     2521 ccgccttggt cggaagcctt gccgccacgg cccgcgccgc cgccgccgtc ggggcgggcg
     2581 atgcggtcgc cggtgacgaa ttcttcattg cccggatgca ccacctcgcg cgtgccgccc
     2641 gagccatggt gaaaggtggg ctcggacaca tcgcgcaccg ggatggagac tttttcgccg
     2701 ctggaaatat ccgtaatgct gcgccccttg atggcgcgcg ccacggactg gcggatttgc
     2761 gcgcgaaagc gccgtaaaaa gcgctcgcgg ttcaccgccg acttgttctt gccgttgagc
     2821 cgtctgtcga tgaggtgaga catggttgcg tccttcactg agtcggagcc gcgcgcgcgg
     2881 caagcgcacg ccgggcgact ccggtaagcc ggggcccgcg ccgccagctc aggggctgac
     2941 ggcgcgggcg ggggtgcgcc ggaccgcgat caggacgatt ttctgacgcg cagataccat
     3001 tcgcacaaca ggcgcacct
//
LOCUS       DAOPYX010000340         3017 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_44800_length_3017_cov_11.589129, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000340 DAOPYX010000000
VERSION     DAOPYX010000340.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 3017)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3017)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3017
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44800_length_3017_cov_11.589129"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1019)
                     /locus_tag="PKN55_13090"
     CDS             complement(<1..1019)
                     /locus_tag="PKN55_13090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011134353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNX72228.1"
                     /translation="MSTPTTLDAAQARDPRSRILGLISLCHCLNDMMQSLILASYPLL
                     KQNHGLSFTQIGLLTLTYQCTSSLLQPLVGLYTDRKPQPRSLMAAMAFTLMGLLLLSQ
                     ASHFPALLCAAALVGMGSSIFHPEASRVARMAAAGGKAGFAQSLFQVGGNAGSALGPL
                     LAATVVYPHGQTSLAAFSAVALVAMVLLNQVSHWYASHPRRHGQRPPVEHGYSRAKVG
                     LILGILLLLVFSKYFYMASMTSYYTFYLMSRFALDVQTAQMLLFVFMFAVALGALLGG
                     PLGDRIGRRRVIWFSILGAAPFTLLLPHANLAGTVALSFGAGMILASAFSSIVVYAQE
                     LLPGRV"
     gene            complement(1729..>3017)
                     /locus_tag="PKN55_13095"
     CDS             complement(1729..>3017)
                     /locus_tag="PKN55_13095"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HNX72229.1"
                     /translation="VKPTLPASHWQAALPADTPQAHGWQDYHSAELLRLQAQALADNP
                     GWQAALSRVAQARASLAAVDGARQPQLNLSGNRASSQTRQQGSEQNTENWQAGASVAY
                     EVDLWGRLAAGSRSAQASAEASEYAAASTRLLLETDVASGYFRLCLLDERLRLNDEDI
                     AAARTILRGLEGKQRVGAASQLDIAQQAQLLATQEAARASLQAQRGAALTALAVLVGA
                     PPQGFAVATQPLAQLTALSPALRQPGELLARRPDIAQAEALLRAADADVDAARAAFFP
                     KLTLSASSLWAASGGGPAGLAASLLAGLSAPLWDGGQLNAGLQRNAARRDELTANYRA
                     SLLTALKEGEDALDGLARQQSRDAALARALTAAEQSYALAQGRLRAGALDGLSLLNAQ
                     RTLIASRDSRAQSRFDSLQAGIALYKALGGGWASEH"
BASE COUNT          531 a          961 c         1001 g          524 t
ORIGIN      
        1 acccggccgg gcagcagctc ttgcgcatac accacaatcg aagaaaacgc cgaggccaga
       61 atcatccctg cgccaaagct cagcgccacc gtgccagcca gattggcgtg cggcagcagc
      121 aaggtgaacg gcgcggcgcc gaggatggaa aaccagatca cccgccgtcg gccgatgcgg
      181 tcacccagcg gcccgcccag caaggcgccc agcgccacgg caaacatgaa cacaaacaac
      241 agcatctggg cggtttgcac atccagggca aagcgcgaca tcaggtaaaa cgtgtaatag
      301 ctggtcatgc tggccatgta gaaatacttg gaaaacacca gcaacagcaa aatgcccagg
      361 atcaacccca cctttgcccg gctatagcca tgctcgaccg gtgggcgctg gccgtggcgg
      421 cgcggatgac tggcgtacca gtggctgacc tggttcaaca gcaccatggc caccagcgcc
      481 accgcagaaa acgccgccag gctggtctgg ccgtgtggat agaccacggt ggccgccagc
      541 agtggcccca gtgccgagcc ggcattgcca cctacctgaa acagtgactg ggcaaagccg
      601 gccttgccgc ccgcggcggc catgcgcgcc acccgcgagg cttccggatg aaagatggat
      661 gagcccatgc ccaccagcgc ggcggcacac agcagagcgg gaaagtggct ggcctgcgag
      721 agcagcaaga gtcccatcag ggtaaaggcc atcgccgcca tcagcgagcg cggctgtggc
      781 ttgcggtcgg tatacaagcc caccagcggt tgcaacagcg atgaggtgca ctggtaagtg
      841 agtgtcaaca ggccaatctg ggtaaaactc aggccatgat tctgtttcag cagcgggtag
      901 ctggccagga tcagcgattg catcatgtcg ttcaggcagt gacacaggct gatcaagccc
      961 agaatgcgcg agcgagggtc acgcgcctgg gccgcgtcga gtgtggtggg ggtggacatc
     1021 agaactctcc agcaagacag caaggtggag gccgggtcag cgcggcttgc ctgaagcctg
     1081 gcggctgact gagcaccgct tgcttgactg agcgtcgtgg tcttggctgg gtaggcagca
     1141 gacagcacac gtgatgcggc caggccgcac tgtctacact cacccaccag aagcactgtg
     1201 ctacgttttg tgaacggtgc ttggcctcat atttggcaga aatagtccgg ccataagcaa
     1261 tttttatact gtcagacaaa aaccattgct ttgtttctgg tgcgaactca gcgaaaatga
     1321 acgtctgatt tgcgctggca aatcagtggg ggatggtggt tcagcatgtg tgccgtgtga
     1381 tgaattgcaa ggcgtatgtg cgggatgctc tgcccgtgga gggaaggcgg taacgcgtgt
     1441 ctacatgtca aagcaataca acaatattct tgtaattcta caaggtgggt tgtgcgcttg
     1501 ccattcgtat atttacttag ttttcgtgct gagacgaact tctttggcga atttcgtcaa
     1561 cagcatgctt ctgatctgtc ccctcttgcc cgcgtggcca atcacgccac tctcctggcg
     1621 tcgttgcccg accttgccgt acgacttgta ctgtctgcgg tgatcgcttc gctgagtttg
     1681 ctgagcggcg cgcagcgcag gtgtgtcaca tgacggcgcg cgcacggctc agtgctcgct
     1741 ggcccagccg ccgcccagcg ccttgtacaa ggcaatgccg gcttgcaggc tgtcaaagcg
     1801 cgattgcgcg cggctgtcgc ggctggcgat cagtgtgcgc tgggcattca acaaagacag
     1861 gccgtccagc gcgccggcgc gcagccggcc ttgcgccagc gcgtagcttt gctcggccgc
     1921 cgtcagcgcc cgcgccagcg cggcgtcgcg gctttgctgg cgggccaggc catccagcgc
     1981 gtcttcgcct tctttcagcg cggtcaacag gctggcgcgg tagttggcgg tcagttcgtc
     2041 gcggcgggcg gcgttgcgct gcaagccggc gttcagttgg ccgccgtccc acagcggcgc
     2101 cgacaagccg gccagcaaag acgccgccag cccggccggc ccgccgccgc tggctgccca
     2161 caggctgctg gcgctgaggg tgagcttggg gaaaaacgcc gcccgcgccg cgtccacatc
     2221 ggcatcggcg gcgcgcaaca gcgcctcggc ctgggcgatg tctggccgac gggccagtag
     2281 ctcgcccggc tggcgcaagg ccgggctgag cgccgtcagc tgcgccagcg gctgggtggc
     2341 cacggcaaag ccctgcggcg gcgcgcccac cagcaccgcc agcgcagtca gcgccgcgcc
     2401 gcgttgtgcc tgcaggctgg cgcgcgcggc ttcttgcgtg gccagcaatt gcgcctgctg
     2461 ggcgatatcc agctgcgaag cggcgccgac gcgctgcttg ccttccagcc cacgcaggat
     2521 ggtgcgggcg gcggcaatgt cctcgtcgtt cagccgcagg cgctcgtcca gcaggcacag
     2581 ccgaaaatag ccactggcga cgtcggtttc cagcagcagc cgggtgctgg cggcggcgta
     2641 ttcactggcc tcggcgctgg cttgtgccga gcggctgcct gcggccagcc ggccccacag
     2701 atcgacttcg taggccacgc tggcgccggc ctgccagttt tcagtgtttt gctcgctacc
     2761 ttgctggcgg gtctggctgc tggcgcggtt gccgctgagg ttcagctgcg gctggcgggc
     2821 gccgtccacc gccgccaggc tggcgcgcgc ctgcgccacc cggctgagcg ccgcttgcca
     2881 gccggggttg tcggccagcg cctgcgcttg caggcgcagc aattcggcgc tgtgataatc
     2941 ctgccagccg tgggcctgcg gcgtgtcggc cggcagggcg gcttgccagt ggctggccgg
     3001 cagcgtgggt ttcacca
//
LOCUS       DAOPYX010000341         2977 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_45720_length_2977_cov_12.488022, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000341 DAOPYX010000000
VERSION     DAOPYX010000341.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 2977)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2977)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2977
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45720_length_2977_cov_12.488022"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            170..2200
                     /locus_tag="PKN55_13100"
     CDS             170..2200
                     /locus_tag="PKN55_13100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014087252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS-domain containing protein"
                     /protein_id="HNX72230.1"
                     /translation="MSFDTRPETDRLFREMVQAGLDLLDPGLTVFDHELRMVAWNRSF
                     LRLLDFPESLAVAGMPFEAFIRYNAERGEYGPGDIDAQVAERVHAARSFRAHYTERTR
                     PNGRVLAIRGAPLPHQGFITLYTDITEQKLYRELVQRQNAELEGHVRARTNELETAYT
                     RLTQAMAANAEITSALKRSEARLRLITDTIPAHIAYFDHRWTYQYANRGYARWFGWQP
                     EDVVGKPIDTIIGAQVFATVKEHVSIALAGQQVTYEYSMDLRDGRTVYARSTLVPEID
                     ANGVTLGCFVHSFDITEQRRTQAALVQAQKMEAIGQLTGGLAHDFNNMLTVVTGNLQA
                     LRQQLGERSEVGDYLDPAMQAAQRGVELIKRLLTFSRQQPLEPQPVEVNSLIRGMAQL
                     MRRSLPQSIAITPFAHEDDLVVMADPHQLENALLNLALNARDAMPNGGELRIESSGEQ
                     LNAEDAADIEAAPGEYVQITVSDNGIGMDVGTLTRVFEPFFTTKQFGAGSGLGMSMVY
                     GFVKQSGGGVLLRSRQARGTSVALLLPRTRQTPRDAERPQAATSREDGRGQLVLLVED
                     NPEVRKVARLLLTDLGYPVLEAENALEAADMIENIPDIAILLSDIVMPGGMDGAALAR
                     FARRFRPAMRIVLVSGYAKGLEDDDAEIHGFRFLPKPFTREALAQALRDTLG"
     gene            2361..>2977
                     /locus_tag="PKN55_13105"
     CDS             2361..>2977
                     /locus_tag="PKN55_13105"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNX72231.1"
                     /translation="MAEDLHKRICLVEDDPHVARLIGATLRDFGFAHDWRRTGADLLR
                     LLRSGEVPDLCIVDLGLPDMDGMELVRALRSDGRCGVLILTGRGDTIDRVMGLELGAD
                     DYMVKPFEPRELIARVRSILRRCTAPRDATPLPLSTVAEFAGWRFAPGSNTLSSADGE
                     SSTLSTAEAQLLKTMLQHPNRILTREQLMGERNLSSFDRSIDVRIS"
BASE COUNT          578 a         1002 c          908 g          489 t
ORIGIN      
        1 taaaagggtt ttttgcagtg cacacacgcc gcactggcca tgcggatgag gccagcacaa
       61 catcttgtgg ctgaacacga cagaatttat cttgaatgca agacaaattc cgccccacac
      121 gcccggccgg cgtacactga cggtttgcgc atccaccaca agggccgtca tgagctttga
      181 taccagacca gaaaccgacc ggctgtttcg cgagatggtg caagccgggc tggatttgct
      241 cgaccccggc ctgaccgtgt tcgaccacga gctgcgcatg gtggcgtgga accggtcgtt
      301 tttgcgcttg ctggattttc ccgaatctct ggcggtggcc ggcatgccgt ttgaggcgtt
      361 tatccgctac aacgccgagc gcggcgaata cgggccgggc gacatcgacg cacaagtggc
      421 cgagcgcgtg cacgccgcgc gcagctttcg cgcccactac accgagcgca cccgccccaa
      481 cggccgggtg ctggccatac gcggcgcgcc cttgccgcat cagggtttta tcaccctgta
      541 caccgacatc accgaacaaa aactctatcg cgagctggtg cagcggcaaa acgccgagct
      601 ggaaggccat gtgcgcgcgc gcaccaacga gctggaaacc gcctacaccc ggctgaccca
      661 ggccatggcc gccaatgccg aaatcaccag cgcgctcaag cgcagcgagg cgcggctgcg
      721 gctgatcacc gacaccattc cggcgcacat tgcctatttt gaccatcgct ggacctacca
      781 atacgccaat cgcggctacg cgcgctggtt tggctggcag ccggaagacg tggtgggcaa
      841 gcccatcgac accattattg gcgcccaggt gttcgccacc gtcaaagagc acgtcagcat
      901 cgccctggcc ggccagcaag tcacttacga atattccatg gacctgcgcg acggccgcac
      961 cgtctacgcg cgcagcaccc tggtgccgga aatcgacgcc aacggcgtga cgctgggctg
     1021 ttttgttcac tcgttcgaca tcaccgaaca gcgccgcacc caggccgcgc tggtgcaggc
     1081 gcaaaaaatg gaagccatcg gccagctcac cggcggcctg gcgcacgact tcaacaatat
     1141 gctcaccgtg gtcaccggca atctgcaagc gctgcgccag caactgggcg agcgcagcga
     1201 agtgggcgat tacctcgacc cggccatgca ggcggcgcaa cgcggcgtcg agctgatcaa
     1261 gcggctgctg accttctcgc gccagcaacc gctggagccg cagccggtgg aggtcaacag
     1321 cctgatccgc ggcatggccc agctgatgcg ccgctcgctg ccgcaatcca tcgccatcac
     1381 cccgtttgcc cacgaagacg acctggtggt gatggccgac ccgcaccaac tggaaaacgc
     1441 cctgctcaac ctggcgctga acgcccgcga cgccatgccc aatggcggcg aactgcggat
     1501 tgaatcgtcc ggcgagcagc tcaacgccga agacgccgcc gacattgagg ctgcgccagg
     1561 cgaatatgtg caaattaccg tcagcgacaa cggcatcggc atggacgtgg gcacgctcac
     1621 ccgggtgttt gaaccgtttt tcaccaccaa acagtttggc gccggcagcg ggctgggcat
     1681 gtccatggtg tatggctttg tcaaacaatc cggcggcggc gtgctgctgc gcagccgcca
     1741 ggcgcgcggc accagcgtgg cgctgctgct gccgcgcacc cggcaaaccc cgcgcgacgc
     1801 cgaacgccca caagccgcca cctcgcgcga ggacggccgc gggcaactgg tgctgctggt
     1861 ggaggacaac ccggaagtgc gcaaggtggc gcgcctgctg ctgaccgacc tgggctaccc
     1921 ggtgctggaa gcagaaaacg ccctggaagc cgccgacatg atcgaaaaca tcccggacat
     1981 cgccattttg ctgtctgaca tcgtgatgcc cggcggcatg gacggcgccg cgctggcgcg
     2041 ctttgcccga cgctttcggc cggcgatgcg cattgtgctg gtcagcggct acgccaaggg
     2101 gctggaagac gacgacgcgg aaatccatgg ctttcggttt ttgcccaagc cattcacccg
     2161 agaggcgctg gcgcaagcct tgcgggacac cctgggctag gccgcgtttg gcacggcgtg
     2221 agcacggccg gcggatcaag cgcaaaccac cctcagtatg ccgaagggtt tggtcagcta
     2281 caggaagggc ggtacactct cagtctatcg caagtagaaa tacgggtggc gcgccgccca
     2341 cactcaagga acaacggcat atggctgaag atttgcacaa gcgcatctgc ctggtggaag
     2401 acgacccgca tgtggcccgc ctgattggcg ccacgctgcg cgattttggc tttgcccatg
     2461 actggcgacg caccggcgcg gatttattgc ggctcttgcg cagtggcgaa gtccccgatt
     2521 tatgcattgt tgacctcggc ctgccggaca tggacggcat ggagctggtg cgcgccctgc
     2581 gcagcgacgg ccgttgcggg gtgctgatcc tcaccggccg tggcgacacc atcgaccggg
     2641 tgatgggcct ggagctgggc gccgatgact atatggtcaa gcccttcgag ccgcgcgaac
     2701 tgattgcccg agttcgcagc atcctgcgcc gctgcaccgc cccacgcgac gccaccccgc
     2761 tgccactgtc caccgtggcc gaatttgccg gctggcgctt tgcccccggc agcaacaccc
     2821 tgtccagcgc cgacggcgaa agctccaccc tcagcaccgc tgaggcgcaa ctgctgaaaa
     2881 ccatgctgca acaccccaac cgcatcctga cgcgcgaaca attgatgggt gaacgcaatt
     2941 tatcctcgtt cgaccgcagc atcgacgtgc gcatctc
//
LOCUS       DAOPYX010000342         2974 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_45795_length_2974_cov_8.180199, whole genome shotgun sequence.
ACCESSION   DAOPYX010000342 DAOPYX010000000
VERSION     DAOPYX010000342.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 2974)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2974)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2974
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45795_length_2974_cov_8.180199"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..345
                     /locus_tag="PKN55_13110"
     CDS             <1..345
                     /locus_tag="PKN55_13110"
                     /inference="COORDINATES: protein motif:HMM:NF025619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4351 domain-containing protein"
                     /protein_id="HNX72232.1"
                     /translation="GIKMLETNIDRWEAQAIAKGMQQGIQQGMQQGIQRGIQQGMQQG
                     MQRGIQQGVQQGMQLGEALLLQRQLTRRFGELSAAQQATLAAATPAQLETWGDRVLDA
                     SSLDEVFGDTRH"
     gene            complement(502..1311)
                     /locus_tag="PKN55_13115"
     CDS             complement(502..1311)
                     /locus_tag="PKN55_13115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008953928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNX72233.1"
                     /translation="MVPGLECQSHAAHGAPLDAPPLLFVHGAYCAAWVWEEYFLPWFA
                     QRGWHATAISLEGHGDSRGHAWLAALGIDDFARNVHAVVKTMPRPPVLIGHSMGGFVV
                     QRYLEMHRDELPAGVALMASVPPFGINASAWRMLTTAPDLLAALNLFQANEHYHPDLD
                     QAQRLLFSADVDRERLRDWVRRFQPESMRAIFDLSMISLFPVSHLPAVPALVLGAAND
                     QLIAPHEVVATACRLGVTAEILPDMAHLMMLDTHWEEAAKRLEGWLRATWG"
     gene            complement(1311..2717)
                     /locus_tag="PKN55_13120"
     CDS             complement(1311..2717)
                     /locus_tag="PKN55_13120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014086901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HNX72234.1"
                     /translation="MPAKKKTALSRIKLFVLDTNVLMHDPSCLFRFAEHDVFLPMITL
                     EELDGHKKGMSEVARNARQASRFLDELVRDADDIDAGISLKAASHKAATGRLLLQTRA
                     LDGQLPAGLPVGKADNQILGIVMALHLAQPERQVILVSKDINMRIKAAALGLAAEDYF
                     NDQVLEDTDLLYSGAREIDQGFWEKNGKNLKSWQEGGKSYYRMSGPGCANLLLNEILW
                     QGGEAPFAARVLAREGKTITLETLSDYSHSKHSVWGVTARNREQNFALNLLMNPEIDF
                     VTLLGQAGTGKTLLTLAAGLALTLEEKRYSEIIMTRVTVPVGEDIGFLPGTEEEKMAP
                     WMGALDDNLDVLNKSDDDGGDWGRAATRDLIRSRIKVKSLNFMRGRTFLNKFLIIDEA
                     QNLTPKQMKTLITRAGPGTKVVCLGNIAQIDTPYLTEGSSGLTYVVDRMKNWAHAGHI
                     TLQRGERSRLADFAAEVL"
     gene            complement(2807..>2974)
                     /locus_tag="PKN55_13125"
     CDS             complement(2807..>2974)
                     /locus_tag="PKN55_13125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011136362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HNX72235.1"
                     /translation="AAFGVMKLKNMYGKQVRGIERSTFVLGEGGSVLREWRGVKVPSH
                     VAEVLAFVNTL"
BASE COUNT          512 a          891 c          991 g          580 t
ORIGIN      
        1 ggaatcaaga tgctggaaac caatatcgac cgctgggaag cccaggcgat tgccaagggg
       61 atgcagcagg ggattcaaca aggcatgcaa caagggattc aacggggaat tcaacagggg
      121 atgcagcaag ggatgcaacg gggaattcag cagggtgtgc aacaaggcat gcagctgggc
      181 gaggccttgt tgttgcaacg ccagctcacc cgtcgttttg gcgaattgtc cgccgcccag
      241 caggccacgc ttgccgccgc cacgcccgct cagctggaaa cctggggcga tcgggtgctg
      301 gatgcaagct cgctggacga ggtgtttggc gacacgcggc actgaatttc ctggccctcc
      361 gagggccagg ccaagctctt gtcgccaccg gaaccatccc cgtctctctt ctttgccacc
      421 gtggcaagcc gcctcggccg tgatggccgg cagccctgcg ccagacagcg cagggccgct
      481 gtcagcggcc atttggcagc cttatcccca cgtcgcccgc agccatccct ccagccgctt
      541 ggcggcttct tcccaatggg tgtccagcat catcaggtgg gccatgtccg gcaggatttc
      601 ggcggtgacg cccaggcggc aggcggtggc caccacttcg tgcggggcaa tcagttggtc
      661 gttggcggcg cccagcacca gagcggggac ggcgggcagg tggctgaccg gaaacaggct
      721 gatcatcgac aggtcaaaaa tcgcccgcat ggattccggc tgaaagcggc gcacccagtc
      781 gcgcaggcgt tcgcggtcta cgtcggccga gaacagcagg cgctgggcct ggtcgaggtc
      841 ggggtggtag tgctcgttgg cctgaaacag gttcagcgcc gccagcaggt cgggggcggt
      901 ggtgagcatg cgccaggccg aggcgttgat gccaaaaggc ggcaccgagg ccatcagcgc
      961 cacgccggcc ggcaattcat cgcggtgcat ttccaggtag cgctgcacca caaagccgcc
     1021 catcgagtgg ccgatcagca cgggcgggcg cggcatggtt ttcaccacgg cgtggacatt
     1081 gcgggcaaag tcatcaatgc ccagcgccgc cagccaggcg tggccacggc tgtcgccatg
     1141 gccttccagg ctgatggcgg tggcgtgcca gccgcgttgg gcaaaccagg gcaaaaagta
     1201 ttcttcccac acccaggcgg cgcagtaggc gccgtgcaca aacaacagcg gcggggcatc
     1261 cagcggggcg ccgtgggcgg cgtgtgattg gcattccagt ccgggcacca ttacagcacc
     1321 tcggcggcaa agtctgccag ccgcgagcgt tcgccgcgtt gcagggtgat atggccggcg
     1381 tgggcccagt ttttcatgcg gtccaccacg taggtgaggc cggagctgcc ttcggtgagg
     1441 tagggggtgt cgatttgggc gatattgccc aggcacacca ctttggtgcc ggggccggcg
     1501 cgggtgatca gggttttcat ttgtttgggg gtcaggttct gagcttcgtc gatgatgagg
     1561 aatttgttca aaaaagtgcg gccgcgcatg aagttcagtg atttgacctt gatgcggctg
     1621 cggatcaggt cgcgggtggc ggcgcggccc cagtcgccgc cgtcgtcgtc ggatttattg
     1681 agcacgtcca ggttgtcgtc cagcgcgccc atccaggggg ccattttttc ttcttcggtg
     1741 ccgggtaaaa agccgatgtc ctcgcccacc ggcacggtga cgcgggtcat gatgatttcg
     1801 ctgtagcgtt tttcttccag cgtcagcgcc aggccggcgg ccagggtgag cagggttttg
     1861 ccggtgccgg cctggcccag cagggtgaca aaatcaatct ccgggttcat cagcaggtta
     1921 agggcaaagt tttgctcgcg gttgcgcgcg gtcacgcccc atacgctgtg cttgctgtgg
     1981 ctgtagtcgc tgagggtttc cagcgtgatg gttttgcctt cgcgcgccag cacgcgggcg
     2041 gcaaacggcg cttcgccgcc ttgccagagg atttcgttca gcaagaggtt ggcgcagccg
     2101 gggccgctca tgcggtaata gcttttgccg ccttcttgcc aggatttgag gtttttgccg
     2161 tttttttccc agaagccttg gtcgatctcg cgcgcgccgc tgtacagcag atcggtgtct
     2221 tccagcactt gatcattgaa atagtcttct gccgccaggc ccagcgcggc ggccttgatg
     2281 cgcatattga tgtctttgga caccaaaatc acctggcgct cgggctgggc caggtgcaag
     2341 gccatgacaa tgcccagaat ctggttatcc gccttgccca ccggcaggcc ggccggcaat
     2401 tggccatcca gcgcgcgggt ctgcagcagc agccggccgg tggcggcctt gtggctggcg
     2461 gctttcaggc tgatgccggc gtcgatgtcg tcggcgtcgc gcaccagttc atccagaaaa
     2521 cggctggcct gacgggcgtt gcgcgccact tccgacatgc cttttttgtg gccgtcgagt
     2581 tcttccaggg tgatcatcgg caagaacaca tcgtgttcgg caaagcggaa caggcaggaa
     2641 gggtcgtgca tcagcacatt ggtgtccagc acaaacagtt tgatccggga gagggcggtt
     2701 tttttcttgg ctggcatgag agtgggcttt cctgttggcg caatatcagt cgaaaagcgg
     2761 ccaggcgacg cgcgcccggc ggccagcaca gcatgcgcgg cgcgcgctac agcgtgttga
     2821 caaaggccag cacctcggcc acatggctgg gcacttttac gccgcgccac tcgcgcagca
     2881 cgctgccgcc ttcgcccagc acaaaggtgc tgcgctcgat gccgcgcacc tgtttgccgt
     2941 acatgttttt cagcttcatc accccgaacg ctgc
//
LOCUS       DAOPYX010000343         2973 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_45812_length_2973_cov_8.870459, whole genome shotgun sequence.
ACCESSION   DAOPYX010000343 DAOPYX010000000
VERSION     DAOPYX010000343.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 2973)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2973)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2973
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45812_length_2973_cov_8.870459"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1287)
                     /locus_tag="PKN55_13130"
     CDS             complement(<1..1287)
                     /locus_tag="PKN55_13130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011134488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNX72236.1"
                     /translation="MKSSRLTVAPATAQKLARLGLVEPMDLVLHLPLRYEDETRITAI
                     ADAPYQHSVQIEGEVRQCEVRRQPRKQLRVLLADASGAVWLRFLHFYPSQEKLCAVGQ
                     RLRAVGEVRRGFQGDEMVHPKIHAVGDNDVLPEAMTPVYPTVQGLNQSTLRRLIGQAL
                     ALEDLSDTLPAALRAELALPDFADAVHTLHHPPPDCAQQALALRRLPAWQRLKFDELL
                     AQQLAMRILYRQRRHGGSPRLCGDGRLRQALLAQLPFALTAAQQRVLAEIDADLASPH
                     PMRRLLQGDVGSGKTVVAALAALTAIEAGYQAALMAPTELLAEQHYLKLRDWLTPLGL
                     TVAWTSGSQKKSERQAARAAMASGAAQLTVGTHALFQDDIDFARLGLAIVDEQHRFGV
                     GQRLALSGKGGEPHQLMMSATPIPRTLAMSYYADLDV"
     gene            complement(1635..2072)
                     /locus_tag="PKN55_13135"
     CDS             complement(1635..2072)
                     /locus_tag="PKN55_13135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014085958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2147 domain-containing protein"
                     /protein_id="HNX72237.1"
                     /translation="MQFATRALCLAASLTLSSLALANNDSPVGTWKTIDDETKQAKAL
                     VQISESGGVLTGKIIKLFANPDALCDKCDGERKDKPVQGMTILWDLKKNGDAWEGGKI
                     LDPKKGQVYSSKAKLVDGGAKLEVRGFMGVSLLGRSQVWERQP"
     gene            2406..>2973
                     /locus_tag="PKN55_13140"
     CDS             2406..>2973
                     /locus_tag="PKN55_13140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012697351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HNX72238.1"
                     /translation="MTSRKPFYRSLYFQVIVAILLGVLLGHFWPQTGASLKPLGDGFI
                     KLVKMMIAPIIFCTVVTGIAGMEDMKKVGRVGGKALLYFEVVTTLALAIGLIVVNVVK
                     PGAGMHVDPATLDASAVAKYTSKASEQSTVDFIMHIIPNSVVGAFAEGEILQVLLFSV
                     LFGFALSALGQRARPVVNLLEQISHGLFV"
BASE COUNT          455 a          964 c          974 g          580 t
ORIGIN      
        1 cacatccagg tcggcgtagt agctcatcgc cagcgtgcgc ggaatcggcg tggcgctcat
       61 catcagctgg tgcggctcgc cgcccttgcc gctgagcgcc aggcgctggc cgacgccaaa
      121 gcggtgctgt tcatcaacaa tcgccagccc cagccgggca aagtcgatgt cgtcctgaaa
      181 cagcgcgtgg gtgcccaccg tcagctgcgc cgcgccgctg gccatcgccg cgcgcgccgc
      241 ctggcgttcg ctttttttct ggctgccaga ggtccacgcc acggtgagcc ccagcggggt
      301 cagccagtcg cgcagcttga ggtagtgttg ctcggccagc agctcggtgg gggccatcag
      361 cgccgcctga taaccggcct cgatggcggt cagcgccgcc agcgccgcca ccacggtttt
      421 gccgctgccg acatcgccct gcaacagccg gcgcatcggg tgcgggctgg ccaggtcggc
      481 gtcgatttcg gccagcacgc gctgctgggc ggcggtcagc gcaaacggca gctgcgccag
      541 caaggcctgg cgcagccggc catcgccgca cagacgcggc gagccgccat ggcggcgctg
      601 gcgatacaga atgcgcatgg ccagttgctg ggccagcagc tcgtcaaatt tcagccgctg
      661 ccaggccggc agcctgcgca gggccagcgc ctgctgagcg cagtcgggcg gcgggtggtg
      721 cagggtgtgc accgcgtcgg caaagtcggg cagggccagc tcggcgcgca gcgccgccgg
      781 caaggtgtcg gacaggtctt ccagcgccag cgcctggccg atcagccggc gcagcgtgct
      841 ttggttcagc ccctgcacgg tggggtagac cggggtcatc gcctcgggca gcacgtcgtt
      901 gtcgccaacg gcgtggattt tcgggtgcac catttcgtca ccctgaaacc cccggcgcac
      961 ctcgcccacc gcgcgcaggc gctggccgac ggcgcagagt ttttcctggc tgggataaaa
     1021 atgcagaaag cgcagccaca ccgcgccgct ggcgtcggcc agcaacacgc gcagctgttt
     1081 gcgcggctgc cggcgcacct cgcactggcg cacctcgcct tcgatctgca cgctgtgctg
     1141 atagggggcg tcggcaatgg cggtgatgcg ggtttcgtcc tcgtagcgca gcggcaggtg
     1201 caacaccaga tccatcggct cgaccaggcc cagacgggcc agcttttgcg ccgtggccgg
     1261 cgcgactgtc aggcgggagc ttttcatgcg gctattgtac ccatgccagc aaaaaaaacg
     1321 gcgcgccggt ttgtcgccgg tcacgccgtt tcaaccccac tgatgcacga gccggcggga
     1381 gagggcgctg gctcgttttc ccctgcgtca gccctggcgg actgacaggc acagtatgct
     1441 cgccccgctg tctggcgccg tgttggcgcg ggtcagcggg gtgggcagac atggatgaat
     1501 gcggtgatgc gagctcgccc ggataggccg agtgtggcca ccgcactgaa aaacgggcgc
     1561 cgcagcgccc gtcaggctga atcgtcgcgc caaggctgcc gccccgcagc gcctggtgcg
     1621 tcaggtgatg ctttttacgg ttggcgttcc cacacttgcg agcgacccag cagcgacacc
     1681 cccataaagc cgcgcacttc cagcttggcg ccgccgtcca ccagcttggc cttgctgctg
     1741 tacacctggc cttttttcgg gtcgaggatc ttgccccctt cccaggcgtc gccgttcttt
     1801 ttcaggtccc acagaatggt catgccttgc accggcttgt ctttgcgctc gccgtcgcat
     1861 ttgtcgcaca gcgcgtccgg gttggcgaac agcttgatga tcttgccggt gagcacgccg
     1921 ccggattcgc tgatttgcac cagcgccttg gcctgcttgg tttcgtcgtc gatggttttc
     1981 caggtgccca ccggagagtc gttattggcc agcgccagcg acgacagggt gaggctggcg
     2041 gcaaggcaca gcgcgcgagt ggcaaattgc atggttaggt ctcctctatc ttcctcgtgt
     2101 tgactcgatg gcctggcgcg tgcgttgcgc gccgtgtgcg caattcgggt tcgccggatc
     2161 ggcgaaattc aaacgttggt ttgaatgtgc ggccaagcat gccgcagctg tggggactcg
     2221 tcaagcgcag cgctaccgcc tgaatcacgg ttcattaggg ttttcccgaa taggggcagc
     2281 cctaatttca tattgcaaca aatccgcgca atatcgccgc cggttacccg caatactgtg
     2341 gccgctgccg cgctccccct gattggcgcg gcggcgggtt ttttgcccct ccggagtccg
     2401 cgccgatgac gtcccgcaaa ccgttttacc gttctcttta cttccaggtg attgtcgcca
     2461 ttctgctggg cgtgttgctt ggtcatttct ggccgcaaac cggcgcttcg ctcaagccgc
     2521 tgggcgatgg cttcatcaag ctggtcaaaa tgatgatcgc gccgatcatt ttctgtaccg
     2581 tggtcaccgg catcgccggc atggaagaca tgaaaaaagt cggtcgggtg ggcggcaagg
     2641 cgctgctgta ttttgaagtg gtcaccaccc tggccctggc catcggcctg atcgtggtga
     2701 atgtggtcaa acccggtgct ggcatgcatg ttgacccggc cacgctggac gccagcgcgg
     2761 tggccaagta caccagcaag gccagcgaac agtccaccgt ggacttcatc atgcacatca
     2821 tccccaatag cgtggtgggc gcctttgccg aaggcgagat tctgcaggtg ctgctgttct
     2881 cggtgctgtt tggctttgcc ctgtcggcgc tgggccagcg tgctcgcccg gtggtgaatc
     2941 tgctggagca aatctcgcac ggcctgtttg tgg
//
LOCUS       DAOPYX010000344         2971 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_45874_length_2971_cov_3.040123, whole genome shotgun sequence.
ACCESSION   DAOPYX010000344 DAOPYX010000000
VERSION     DAOPYX010000344.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 2971)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2971)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2971
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45874_length_2971_cov_3.040123"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            267..>1954
                     /locus_tag="PKN55_13145"
     CDS             267..>1954
                     /locus_tag="PKN55_13145"
                     /inference="COORDINATES: protein motif:HMM:NF025214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNX72239.1"
                     /translation="MTQTDSLFAQALAHHEAGRGNDAEPLYLAVLAESPTHPHALHNL
                     GQLWLGRGEPARALSYLRRALELVPEDGLFWVSCLEALLHDERYPEASELLARGRAQG
                     LAGEVVEQLAQALDFARDLPRHDAAIVACFQQGDDLQLAECARAVLAQCPGHGFAWKA
                     LGVALIRLGQFSEAMQALDMAQRRLPDDASVFNSLTNVLNSLGRHEEAEVAGRRALAL
                     QADYPQALVNLGNALRGMACLEEAERYYRQAMRTPACQIEAGNNLGIVLEEQGRLDEA
                     ESCLRDVLEHDPDYAPAWNHLGCVLDEQGRHEAALQAFQRCLSLDPDNVMAYNNLACT
                     LKSLQRFTEAEQALSHALQLAPSDYEALNNLGDLLKELGRTETASEAFWQAWQVDVSR
                     PTALHNLLVLAQYVEHISDAERISLSQAFARQYEPEVKSWGAWPPLNDPERPLRLGWV
                     SADLRNHSVAYFSLPVIEGLVGKFEQYVYYNSHDHDPIQVRHQHACTVWRDVAGWSDH
                     ALATQIGTDQIDILIDLSGHTARNRLLTFARGAAPVQVTWLGYPDTTGLHNMHWR"
     assembly_gap    1955..1964
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2039..2707
                     /locus_tag="PKN55_13150"
     CDS             2039..2707
                     /locus_tag="PKN55_13150"
                     /inference="COORDINATES: protein motif:HMM:NF025214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX72240.1"
                     /translation="MLRHPAQRLAAEYAVRQTPALTTGQITLGCCNNLTKLTPGVIRV
                     WARVLLAIPGSRLLLELAGAQHELLCHALLNAFSSHGVNPDRVCLLPRDSTRQYLTYH
                     DIDIALDPFPANGGASSCDTLWMGVPLVTLAGQRFASRMGCGLLHAVGHPEWVATSED
                     EYVKIVVELARDLHSLNALRLGLRTQVEASPLMDEARFCLDFTSALRGMWRQACIERV
                     SVSR"
BASE COUNT          568 a          748 c          897 g          748 t
ORIGIN      
        1 tgggcgcgga cccgacgctg aagaattttg acgactggac cgcgctggat ctggctgcgt
       61 cgccagggtg tttgcgggcc ttgcgcaagc ggcggccata gcattggcct tgccactatg
      121 gacgtcttga tgtgcttgtg gtcatattca gtctggcagc acgccatcgg tttgatggca
      181 tatgtgtttt ccttgctgtt ttttcctcac atatcacacg cgtcggtcgg ggctgttgtc
      241 ggcaagctgg cggacgcagg catatcatga cacagactga ctctctgttt gcccaagcac
      301 tggctcatca tgaagctggt cgtggcaacg atgctgaacc tttgtatctg gccgtgctgg
      361 ctgaatcacc aacccatcca catgcgttgc acaacctcgg gcagttatgg ctgggacgtg
      421 gtgaacctgc ccgtgcgctg tcgtatctgc gccgggcgct ggagcttgtg ccagaagatg
      481 gcttgttctg ggtgagttgc ctggaagcct tgttgcatga tgagcgctat cccgaagcga
      541 gcgaattgct ggcgcgcggc cgggcgcaag ggttggcggg ggaggtggtg gagcagttgg
      601 ctcaggcgct ggattttgcc cgtgacttac cacgacatga tgcggcgatt gttgcctgct
      661 ttcaacaagg agatgatctg caattggccg aatgtgcccg tgccgtcctg gcacagtgcc
      721 ctggacacgg ttttgcctgg aaagctctgg gtgtggcgct gattcgtttg gggcagttct
      781 ccgaggcgat gcaagcactg gatatggcgc aacgcaggtt gccagatgat gcctctgtat
      841 ttaatagctt gaccaatgtg ctcaacagtc tggggcgtca tgaagaggcg gaggtggcag
      901 ggagacgtgc gcttgccctg caggctgatt acccacaggc cttggtcaat ctaggtaatg
      961 ccttgcgagg aatggcgtgc ttggaagaag ctgagcgcta ttatcgccaa gccatgcgta
     1021 cgcctgcttg ccagattgag gctgggaata acctgggtat tgtattggaa gaacaggggc
     1081 ggctggacga ggcagaatcc tgtttgcgtg atgtgctgga acatgatccg gactatgccc
     1141 cagcctggaa tcatctggga tgtgtgttgg atgagcaggg acgtcatgag gctgccttgc
     1201 aggcatttca gcgctgtctc tctctggatc cagataatgt catggcatat aacaacctgg
     1261 catgtactct caagtcattg caacgtttta ccgaggctga acaggcactc agccatgccc
     1321 tgcagttggc cccctcagat tatgaggcgc tgaacaattt aggcgattta ctcaaagagc
     1381 tggggcgtac agaaacagcg agtgaggctt tctggcaggc ttggcaagtc gatgtctcgc
     1441 ggccgaccgc gctgcataat ctgttagtgc ttgcccagta tgtcgagcat atcagtgatg
     1501 ccgaacggat ttctctgagt caggcgtttg cacggcagta tgagcctgag gtgaagtctt
     1561 ggggggcttg gccaccgttg aatgatccgg aaaggccttt gcggttgggc tgggtgtctg
     1621 ccgatttgcg caatcattct gtggcctatt tctcgctgcc tgtcattgag gggttggtcg
     1681 gcaaatttga gcagtatgtg tactacaaca gccatgatca tgatcccatt caagtccgcc
     1741 accagcatgc ttgtacagtg tggcgtgatg tcgcaggctg gagcgaccat gcgctggcca
     1801 cccagatcgg cacggatcag attgatatcc tgattgatct ttctggtcat actgcccgta
     1861 atcgtttact gacatttgcg cgtggtgcgg cgcctgtgca agtaacatgg ctgggatatc
     1921 ctgataccac gggcttgcac aatatgcatt ggcgnnnnnn nnnncctcac gatgaaaaca
     1981 atgagcatgc ttgtactgct tgtggtattt gccagatgtc ttttgctgct accgcccgat
     2041 gttacgccac cccgcgcagc ggctggctgc agagtatgca gtacgccaga ccccggcatt
     2101 aacaacaggc caaattacat tgggctgctg taataattta accaaactga cgcccggagt
     2161 cattcgtgtg tgggcccgtg tgctattggc tattccaggc agccgtctgt tactggagct
     2221 ggctggtgct caacatgagc tcttgtgcca tgcgctcttg aatgcatttt ccagtcatgg
     2281 tgtaaacccg gatcgggtat gtctcttgcc acgtgacagt acccgtcaat atctgactta
     2341 tcacgatatt gatattgcat tggatccttt tcctgccaat ggtggggcgt ccagctgtga
     2401 cacattatgg atgggtgtgc cattggttac ccttgccggc cagcggtttg cttcccgcat
     2461 gggctgcggc ctgctgcatg ctgtgggtca ccctgaatgg gtggccactt ccgaggacga
     2521 atatgtcaag atcgtcgtgg agttggcccg cgatctgcat agccttaatg ctttgcgctt
     2581 ggggctgcgt actcaggtgg aggcgagccc gctgatggat gaggcgcgtt tttgcctgga
     2641 ttttacctcg gccttgcgcg gaatgtggcg gcaggcatgt attgagcggg taagcgtctc
     2701 acggtgacgt gtgtctaaaa gtcactcaca aaacccttct ggcgtagtag cccgatcttg
     2761 ccactttttg cactgtctgc gctcgcctac ctggccagaa ccaggacgct aagtatggct
     2821 ggccgtattg tccacccaga acaaagccgc ccgagccggg cggctttttc gtggctaagt
     2881 gttgttcgct acactcagca caatgttcct ggccaagcgc acgaatcagt acggcaaggt
     2941 cgggcaacac gccatgaggg ggttgttagc c
//
LOCUS       DAOPYX010000345         2930 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_46840_length_2930_cov_6.724522, whole genome shotgun sequence.
ACCESSION   DAOPYX010000345 DAOPYX010000000
VERSION     DAOPYX010000345.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 2930)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2930)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2930
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46840_length_2930_cov_6.724522"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1075)
                     /locus_tag="PKN55_13155"
     CDS             complement(<1..1075)
                     /locus_tag="PKN55_13155"
                     /inference="COORDINATES: protein motif:HMM:NF015316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HNX72241.1"
                     /translation="MKLNTLSLSIMTMGALAGASLTAHASGYHFGTQSVSAQATANAS
                     AAEATDATTLFYNPAGMTRLQGTNFSGALNLVAPSVKYNNASASYPTQPYAAQPLPNG
                     VTVPAGLNRSSAVSGSTSGKLTDDLLAVPHMYLTHQLNDRVTVGVGVYVPFASSTEYQ
                     RDSVLRYNVNQTSLTTIDINPTVAFKLNDQHSLAIGLIAQHADASLRQYANFGGSANP
                     ALNSLIPGVGGGHGNSDGYADVEGKDWGFGFNLGWMWEIDPTARVGVSYRSVIKHSLK
                     GTADWTTPATLAGQAAASRGYIDSDVRVDIKTPESLSVHGFKQLDSKWAVMGDVTWTR
                     HSRFDSLSINYSQPKSAPYSASNF"
     gene            complement(1373..1771)
                     /locus_tag="PKN55_13160"
     CDS             complement(1373..1771)
                     /locus_tag="PKN55_13160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018151703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog domain-containing protein"
                     /protein_id="HNX72242.1"
                     /translation="MPDRANAERCELPKDRMPMLRVNAEPRSTNAHGKVHAGWLMAKM
                     DLAGALDAERVAKGPVTTVAVNAFQFAMPILLGDVVSFYVERLRLGQKSITSKVEVFA
                     ERLDGGCVRITEAVMTFVAVDDDGRSRLIS"
     gene            complement(2066..2401)
                     /locus_tag="PKN55_13165"
     CDS             complement(2066..2401)
                     /locus_tag="PKN55_13165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014087611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HNX72243.1"
                     /translation="MPRITFIHPALSEPKILDATSGEALIDVVRRAEVPLYWRCGHGT
                     CCACQVRITHAQQPRDTPWPRKERNVQARHGLIDAATQARDTIDDSPERWRLACSLIV
                     GEEDWEVRW"
BASE COUNT          545 a          878 c          931 g          576 t
ORIGIN      
        1 caaagttgct ggcgctgtac ggcgcggact tgggttggct gtagttgatc gacagcgaat
       61 caaagcgcga gtggcgcgtc caggtgacat cgcccatcac cgcccacttg ctgtccagct
      121 gcttgaagcc atgcaccgac agggattccg gggttttgat gtccacgcgg acgtcgctgt
      181 cgatatagcc gcgcgaggcc gcggcctggc cggccagcgt ggccggcgtg gtccagtcag
      241 ccgtgccctt caggctgtgc ttgatgaccg agcggtagga gacgcccacg cgggcggtgg
      301 ggtcgatttc ccacatccag cccaggttaa agccaaagcc ccagtccttg ccctcgacat
      361 cggcgtagcc gtccgagttg ccatggccgc cgcccacgcc cggaatcagg ctgttcagcg
      421 ccgggttggc gctgccgcca aagttggcgt actggcgcag cgaggcgtcg gcgtgctggg
      481 cgatcaggcc gatggccagc gagtgctggt cgttgagctt gaaggccacg gtcgggttga
      541 tgtcgatggt ggtcagcgag gtttggttga cgttatagcg cagcaccgag tcacgctggt
      601 attcggtgga cgaggcaaac ggcacataca cgcccacgcc caccgtcacg cggtcgttga
      661 gctggtgggt caggtacatg tgcggcacgg ccagaaggtc gtcggtcagc ttgccgctgg
      721 tggagccaga cacggcgctg ctgcggttca ggccggccgg tacggtcacg ccattgggca
      781 ggggctgggc cgcataaggc tgggtggggt agctggcgct ggcgttgttg tacttgacgc
      841 tgggtgcgac cagattcagc gcgcccgaga agttggtgcc ctgcagacga gtcatgccag
      901 ccgggttata aaacagggtg gtggcatcgg tggcttcagc agcgctggca ttggccgtgg
      961 cttgtgcact gaccgactgg gtgccgaagt gataaccgga ggcgtgggcg gtcaggctgg
     1021 cgccggccag cgcgcccatc gtcatgatgg acaggctcag ggtgttgagt ttcatgcgtt
     1081 cttcctctta tttccagcgt ggacggacgt tggtgttatg gcttgaggtc atgccgtcga
     1141 gggcatccct gcgtcggata ctagcaaaga ttttttatcg gcgagaagcc tgtttcgtac
     1201 atttattcca aacactcgtt taacacgatg acttgttgta tttacgcaac ttagcacaat
     1261 ttttcatgtt gcgatgcaac aagacccagg ctgccgatct gacggcaagg cttgccagca
     1321 ctggatttca gggtttttgg ctgctggcaa ggcacagtgt tgacaaaaaa cctcagctaa
     1381 tcaaccgcga acgcccgtcg tcgtccactg ccacaaacgt cataaccgct tcggtgatgc
     1441 gcacacagcc gccatccagg cgctcggcaa acacctccac cttgctggtg atggattttt
     1501 gccccaggcg cagccgctcg acgtaaaagc tcaccacatc acccagcaag atgggcatgg
     1561 caaactgaaa ggcattcacc gccacggtgg tcaccgggcc cttggccacc cgctcggcgt
     1621 ccagcgcgcc ggccaggtcc attttggcca tcagccagcc ggcatgcacc ttgccgtggg
     1681 cattggtgct gcgcggctcg gcgttgaccc gcagcatggg catgcggtct ttgggcaatt
     1741 cacaacgttc ggcattggcg cggtcaggca tggcgggtct ccctggctct cagaatagag
     1801 cgtgatttct ggcaaacgat gccgtcagct tagcatcgct ggcggcaagc gccagcaccc
     1861 atcacggaca acgcttttgt catccggcac aagatgccat atctgtttgt attaatcttt
     1921 gatctggaat gaaaaagccc gacaccagcg cttggcctcg cgcacaacac tcagcaaagc
     1981 ggctctggcc aggcggatga ccgcagacag taaaggagta cggcaaagtc atcccacaac
     2041 gccagagggg cttggtgagc aacccttacc agcgcacttc ccaatcctct tcgcccacaa
     2101 tcaggctgca cgccagccgc cagcgctcgg ggctgtcgtc gatggtgtcg cgcgcctggg
     2161 tggcggcgtc gatcaagccg tggcgcgctt gcacattgcg ctctttgcgc ggccagggtg
     2221 tgtcgcgtgg ttgctgggcg tgggtgatgc gcacctggca ggcgcagcag gtgccgtggc
     2281 cacagcgcca gtacagcggc acctcggcgc ggcgcaccac gtcgatcagc gcctcacctg
     2341 aggtggcatc caggattttc ggctcggaca gcgcggggtg aatgaaggtg atgcggggca
     2401 tggtagggat cagcgggcaa aagggccggt gttggcagag ggaattttag accgccccat
     2461 gcgccacgca cagagatact gcgcggggca tggtttatgg tggatcttcc ggcagaccgt
     2521 gagcaaatca cgccgcagcc ctgcgtctac gcgccccatg ttcagaatat ttccgaagga
     2581 aacgcagacg gtgtgcggca ttggctggaa aaaggtgagc ccctgagcgc caactcaaca
     2641 ccccattgat acgagtgtcg tggtctaatc tttcttattc gcccgcgctt atcaatgatg
     2701 cggcccatct cagccaaccg ggcagccctc caagggtcaa tctgccgtac gctgaacatc
     2761 agtcgctcgg gcttgcatca cactttccag caccgtgacc acccggtgtg caagctgcgc
     2821 accgaaaccc tgttcccggc ggcagcctgg aagaggtggc cgactacgcc cccttgtgct
     2881 gaccgccggc cccgccgcgc aactgaccct ggcgcaactg cgcgacctgg
//
LOCUS       DAOPYX010000346         2929 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_46857_length_2929_cov_11.135699, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000346 DAOPYX010000000
VERSION     DAOPYX010000346.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 2929)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2929)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2929
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46857_length_2929_cov_11.135699"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..1593
                     /gene="recQ"
                     /locus_tag="PKN55_13170"
                     /pseudo
     CDS             1..1593
                     /gene="recQ"
                     /locus_tag="PKN55_13170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581744.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
     gene            1688..2905
                     /gene="argJ"
                     /locus_tag="PKN55_13175"
     CDS             1688..2905
                     /gene="argJ"
                     /locus_tag="PKN55_13175"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019921453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HNX72244.1"
                     /translation="MPVNLAPIDPAALLPVAGVTLSYAEAAVKKPGRKDVLLIELAAG
                     SQVAGVFTRNRFCAAPVHLSRAHLASGQPIRALVVNTGNANAGTGEDGRQRALAVCQA
                     VAEQLGCQAEQVLPFSTGVILEPLPFEKIIGALPQRRAGSWADAAEAIMTTDILPKAA
                     SRQVELGGKTVTLTGIAKGAGMIHPNMATMLGFVATDAAIAPELLQGLLSTAADLSFN
                     SITVDGDTSTNDSFMLIATGQAGNAPVSSADSADYQALAAALQSLSIELAQAIVRDGE
                     GATKFMTITVEGGRSREECKAIGYAIARSPLVKTAFFASDPNLGRILAAIGYAGIDDL
                     DVDQLSLYLDDVLVAVNGGRNPAYQEEDGQQVMNKAEILVRVVLGRGDASATVWTCDF
                     SYDYVRINADYRS"
BASE COUNT          524 a          974 c          916 g          515 t
ORIGIN      
        1 ctgatggaag accaggtcgc caccctgtcc gagctgggcg tggcggcggc gtatctgaat
       61 tccactgtcg aagccgacga ggcgcgccgc attgcccagg ccgcgcgcag cggcgagctc
      121 aagcttttat atgtggcgcc cgagcgactg ctgtcgccgc gctttctgga gtttctggat
      181 agcctggatc tggcgctgtt cgccatcgac gaagcgcatt gcgtgtcgca atgggggcat
      241 gacttccggc cggaataccg ccagttgggc gtgttggccg agcgctttcc ggcggtgccg
      301 cgcgtggcgc tgactgccac cgccgacccg caaacccgcg aagaaatcct gcattacttg
      361 cacctgagcg acgctcgggt gtttctctcc agctttgatc ggccgaacat cacctatacc
      421 gtggtggaaa aacaccaggc caagaagcag ctggtcagct atatcgagca gcatcatgcc
      481 ggccagtccg gcattgtcta ttgcctgtcg cgcaagcgcg tggaagacac cgccgcctgg
      541 ctggtggagc aaggcatcac cgccctgcct tatcacgccg gcctgcccgc cgccttgcgc
      601 gccacccacc agcggcgttt tttgcgcgaa gacggcgtgg tgatggtggc caccgtggcg
      661 tttggcatgg gcatcgacaa gccggacgtg cgctttgtcg cccacctgga tttgccgcgc
      721 agcccggaag gcttttatca agaatccggc cgcgccggcc gcgatggcca gccggccgcc
      781 agctggctgt gttatggcct gtcggacatg gtgcagttgc agcagatgtt gttcgagtcc
      841 gagcaggacg aggcgcgcaa acaggttgaa ttgggcaagc tggaagccat gctggcctac
      901 tgcgaaaccg ccgcctgccg ccgccagcta ttgctggcgc actttggcga agccatcgag
      961 ccctgcggca agtgcgacaa ctgcctgaac ccgcccaaga cctttgacgc caccacgctg
     1021 gcgcaaaaag tgttgtcctg tgtgtaccgg gtgaatcagc gctgcgccgc caaccatgtg
     1081 atcgacatct tgctgggccg cgacagtgaa tcggtgcgcc tgcgcggcca ccaaaccttg
     1141 tccacctttg gcgtgggcaa agagctgaat cagcgcggct ggcgttcggt gattcgccaa
     1201 ctggtggcgc agggctatct gaccgtggac gtcagcgcct atcaatcgct caagctcacc
     1261 gaaagctgcc gcgccgtgct gcgcggtgaa acccaggtgt tcatgcgccc gatccgcaat
     1321 gatgcgccgg gcaagtcggt cagcaacgac aacgaccgct ggctgcgcac cgagcgcgaa
     1381 gagcgcatct ggcaagccct gcgcacctgg cgcaaaaaac tggccgacga gcacaatgtg
     1441 ccggcttacg cggtgttctc cgacaagacc ctgcgcgagc tgaccgaacg ccgcccgcaa
     1501 agcgaagccg agctgcgcac catttatggt gtgggcgaga tgaagctggc ccgctatggc
     1561 gaaagcctgc tggaaattct gcgcgaggcg gcggcgtgat ttcgctacaa tcctgccttg
     1621 ccggcgcgtg atgcgcgtcg gcctggattg aatctatcta tatacattga ctgcatcagg
     1681 aaccgacatg cctgtgaacc tcgccccgat tgaccccgcc gccctgttgc cggtggccgg
     1741 cgtcaccctg tcctatgccg aagccgcggt gaaaaaaccg ggccgcaagg atgtgctgct
     1801 gattgagctg gctgccggca gccaggtggc cggggtgttc acccgcaacc gcttctgcgc
     1861 cgctccggtg cacctgtcgc gcgcccatct ggccagcggc cagccgattc gcgcgctggt
     1921 ggtcaacacc ggcaacgcca acgccggcac cggcgaagac ggccgccagc gcgccctggc
     1981 cgtctgtcag gccgtggccg agcaactggg ctgccaggcc gagcaagtgc tgccgttctc
     2041 gaccggcgtg attctcgaac cgctgccgtt cgaaaaaatc atcggcgccc tgccgcagcg
     2101 ccgcgccggc agctgggccg acgccgccga agcgatcatg accaccgaca tcctgcccaa
     2161 ggccgcctcg cgccaggtcg agctgggcgg caagaccgtc accctgaccg gcatcgccaa
     2221 gggcgccggc atgattcacc cgaatatggc caccatgctg ggctttgtcg ccaccgacgc
     2281 cgccatcgcc cccgagctgc tgcaaggcct gctgtccact gcggcggatt tgtcctttaa
     2341 tagcatcacc gtcgatggcg acacctcgac caacgattct tttatgttga tcgccaccgg
     2401 ccaggccggc aacgccccgg tcagcagcgc cgacagcgcc gattaccagg cgctggccgc
     2461 tgccttgcaa tcgctgtcta ttgaactggc ccaggccatc gtccgcgacg gcgaaggcgc
     2521 caccaagttc atgaccatca ccgtcgaagg cggccgctcg cgcgaagagt gcaaggccat
     2581 cggctacgcc atcgcccgct cgccgctggt gaaaaccgcc ttctttgcct ccgaccccaa
     2641 cctgggccgc atcctggcgg caattggcta cgccggcatc gacgatctgg acgtggatca
     2701 gctgtccctg tatctggacg acgtgctggt ggcggtgaac ggtggccgca acccggccta
     2761 tcaagaagaa gacggccagc aggtgatgaa caaggccgaa attctggtgc gcgtggtgct
     2821 cggccgtggc gacgccagcg ccacggtgtg gacctgcgac ttctcgtatg actacgtgcg
     2881 catcaatgca gactatcgca gctgattggc gctgagccgc cgttaagcg
//
LOCUS       DAOPYX010000347         2923 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_47007_length_2923_cov_12.751743, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000347 DAOPYX010000000
VERSION     DAOPYX010000347.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 2923)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2923)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2923
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47007_length_2923_cov_12.751743"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            116..>2923
                     /gene="metH"
                     /locus_tag="PKN55_13180"
     CDS             116..>2923
                     /gene="metH"
                     /locus_tag="PKN55_13180"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018746324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HNX72245.1"
                     /translation="MSTPTAQRIRSLLDSRILILDGGMGTMIQRHQLEEADYRGERFQ
                     DWPCDVKGNNDLLALTRPDVIGGIHQAYLDAGADIIETNTFNATSVAMADYEMESLVW
                     EINHAAAKLVKDLCVAQTARTPEKPRFCAGVLGPTNRTCSISPDVNDPGYRNITFDAL
                     VESYTEAIDGLVKGGADLLLVETIFDTLNAKAAVFAIHQYFDLRPDAERLPIMISGTI
                     TDQSGRTLTGQTTEAFYNSLSHANAVSFGLNCALGPDLLRPYVEEMSRVSATYVSVHA
                     NAGLPNAFGGYDLTPAAMAPQVREWAESGLVNIVGGCCGTTPDHIAAIYQAVKDLPPR
                     PLPQIEAKCRLSGLEPFNIGDKDLFVNVGERTNVTGSRAFAKLILNGDYATALDVARQ
                     QVENGAQVIDINMDEGMLDAHAAMVRFLNLIAAEPDIARVPIMIDSSKWDVIEAGLKC
                     IQGKGIVNSISLKEGKDKFVEQARLLRRYGAAVIVMAFDEAGQADTYARKIEICEKSY
                     RILVDEVGFPPEDIIFDPNVFAVATGIDEHARYGLDFIEATGWIKDHLPHAKISGGVS
                     NVSFSFRGNNKVREAIHAVFLYHAIKNGMTMGIVNAGALEVYDEVDPVLRERIEDVVL
                     MRAPADGSDATENLIALAEKFKGDAVAGKQGEDLAWRELPVAKRLEHALVKGITTYIE
                     QDTEEVRANSARPIHVIEGPLMDGMNVVGDLFGAGKMFLPQVVKSARVMKAAVAYLEP
                     FIEEEKIRLGLQDAPAKGVIIMATVKGDVHDIGKNIVGVVLRCNNYKVIDLGVMVPCQ
                     KILDAAIEHKADIIGLSGLITPSLEEMSHVAKEMQRQGFDIPLLIGGATTSRVHTAVK
                     IAPHYNGPVIYVPDASRAVGVCSNLLSDTLRDAYVQEVADDYAKARAIFEGRGEAKLL
                     PIADAREQRHRIDWARYTPPTP"
BASE COUNT          618 a          951 c          860 g          494 t
ORIGIN      
        1 cccgccgcgc aacgcccgcc agccaccttc tgaacatatg gcgaatgcgt ctgaggcggg
       61 gcggggcgcg tgataaaatc cgggctattt ccatcctgct caagcccctg ccgccatgtc
      121 cacacccact gcccaacgca tccgttccct gctcgactcg cgcatcctga ttctggatgg
      181 cggcatgggc accatgattc agcgccacca gctggaagaa gccgactacc ggggcgagcg
      241 ttttcaggac tggccatgcg atgtgaaggg caataacgac ctgctggcgc tgacccggcc
      301 ggatgtgatt ggcggcattc atcaagccta tctggatgcc ggcgccgaca tcatcgaaac
      361 caacaccttc aacgccacct cggtggcgat ggccgattac gagatggaaa gcctggtgtg
      421 ggagatcaac cacgccgccg ccaagctggt gaaagacctg tgcgtggcgc aaaccgcgcg
      481 caccccagag aaaccgcgct tttgcgctgg cgtgctcggc ccgaccaacc gcacctgctc
      541 gatcagcccg gacgtcaacg accccggcta tcgcaacatc acctttgatg cgctggtgga
      601 aagctacacc gaggccatcg acggcctggt gaagggcggc gctgatctgc tgctggtgga
      661 aaccattttc gacaccctca acgccaaggc ggcggtgttt gccatccacc agtattttga
      721 cctgcgcccg gatgccgaac ggctgccgat catgatttcc ggcaccatca ccgaccaatc
      781 cggccgcacg ctgaccgggc aaaccaccga ggcgttttac aacagcctca gccacgccaa
      841 tgcggtgagc tttggcctga actgtgcgct cggccccgac ctgctgcgcc cctatgtaga
      901 agaaatgtcg cgggtgtcgg ccacttatgt gtcggtgcac gccaacgccg gcctgcccaa
      961 tgcctttggc ggctacgacc tcaccccggc ggccatggcc ccgcaggtgc gcgagtgggc
     1021 ggaatcgggg ctggtgaata ttgtcggcgg ctgttgcggc accacgccgg accacatcgc
     1081 cgccatttat caggcggtga aagacctgcc gccgcgcccg ctgccgcaga ttgaagccaa
     1141 atgccggctg tctggcctgg agccattcaa tattggtgac aaagacctgt ttgtgaacgt
     1201 cggcgagcgc accaacgtca ccggcagccg cgcctttgcc aagctgatcc tgaatggcga
     1261 ctacgccacc gcgctggacg tggcccgcca gcaggtggaa aacggcgcgc aggtgatcga
     1321 catcaatatg gacgagggca tgctcgacgc ccacgccgcc atggtgcgct ttttgaacct
     1381 gattgccgcc gagccggaca tcgcccgcgt gccgatcatg atcgacagct ccaagtggga
     1441 tgtcatcgaa gccggcctga aatgcattca gggcaagggc atcgtcaact cgatctcgct
     1501 gaaagaaggc aaagacaagt ttgtcgagca ggctcgcctg ctgcgccgct atggcgcggc
     1561 ggtgattgtc atggcctttg acgaagccgg ccaggccgac acctacgcgc gcaaaattga
     1621 aatctgcgaa aaaagctatc gcatcctggt ggacgaagtg ggttttccgc cagaagacat
     1681 catctttgac cccaatgtgt ttgccgtggc caccggtatc gacgaacacg cccgctacgg
     1741 cctggacttt atcgaagcca ccggctggat taaagaccac ctgccgcacg ccaagatttc
     1801 tggcggggta tccaatgtgt cgttcagctt ccggggcaat aacaaggtgc gcgaggcgat
     1861 tcacgccgtg ttcctgtatc acgccatcaa aaacggcatg accatgggca tcgtcaacgc
     1921 cggcgcgctg gaagtgtacg acgaggtaga cccggtgctg cgtgaacgca tcgaagacgt
     1981 ggtgctgatg cgcgccccgg ccgacggcag cgacgccacc gaaaacctga ttgccctcgc
     2041 cgaaaaattc aagggcgacg ccgtggccgg caagcaaggc gaagacctgg cctggcgcga
     2101 actgccggtg gccaagcgcc tggaacacgc gctggtgaaa ggcatcacca cctatatcga
     2161 gcaagacacc gaagaagtgc gcgccaacag cgcccggccc atccatgtga ttgaaggccc
     2221 gctgatggac ggcatgaatg tggtgggcga tctgtttggc gccggcaaaa tgtttttgcc
     2281 gcaggtggtg aaatcggcgc gggtgatgaa agccgccgtc gcctaccttg agccatttat
     2341 cgaagaagaa aaaatccgcc tgggcctgca agacgcgccg gccaagggcg tgatcatcat
     2401 ggccaccgtc aagggcgatg tgcacgacat cggcaaaaac atcgtcggcg tggtgctgcg
     2461 ctgcaataac tataaagtca tcgacctggg cgtgatggtc ccctgccaaa aaatcctcga
     2521 cgccgccatc gaacacaagg ccgacatcat cggcctgtcc ggcctgatta cccccagcct
     2581 ggaagaaatg agccacgtcg ccaaagaaat gcagcgccag ggctttgaca tcccgctgct
     2641 gattggcggc gccaccacca gccgcgtgca caccgccgtc aaaatcgccc cgcattacaa
     2701 cggcccggtg atctacgtgc ccgacgccag ccgcgccgtg ggcgtgtgct ccaacctgct
     2761 gtccgacacc ctgcgcgacg cctacgtgca agaagtggcc gacgactacg ccaaagcccg
     2821 cgccatcttt gaaggccgtg gcgaagccaa gctgctgccg attgccgacg cccgcgaaca
     2881 acgccaccgc atcgactggg cccgctacac cccgcccacc ccg
//
LOCUS       DAOPYX010000348         2917 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_47162_length_2917_cov_8.731656, whole genome shotgun sequence.
ACCESSION   DAOPYX010000348 DAOPYX010000000
VERSION     DAOPYX010000348.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 2917)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2917)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2917
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47162_length_2917_cov_8.731656"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..926
                     /gene="hypD"
                     /locus_tag="PKN55_13185"
     CDS             <1..926
                     /gene="hypD"
                     /locus_tag="PKN55_13185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018749472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HNX72246.1"
                     /translation="PIQPANRQHTHRTAGAVEHGVTLCTYGDTLRVPGSGRMSLYKAR
                     AAGGDVRMVYSVDNALALARAEPQRQVVFFAIGFETTTPPTALALLTARAEGLSNFSV
                     YANHVLTPAAMRHLLASGDGVALDGFIGPSHVSTVIGSDAYADIARDSGKPVVITGFE
                     PLDVLIGVRMLIEQINQGRAEVENQFIRAVTATGNRKARQVVDEVFALRPAFDWRGLG
                     EVAASALAIRPEWSAWDAEVKFGLTTHSVADNKACECGAILQGRKKPQDCKVFATVCT
                     PDNPLGACMVSSEGACAAHYAYGRFVSKEPA"
     gene            923..1969
                     /gene="hypE"
                     /locus_tag="PKN55_13190"
     CDS             923..1969
                     /gene="hypE"
                     /locus_tag="PKN55_13190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011516280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein HypE"
                     /protein_id="HNX72247.1"
                     /translation="MSIKPGYTRPLDLKHGRVDMTHGSGGRAMAQLIHELFAAEFGNE
                     WLAQGNDQAIVPPPGGRLAVATDGHVVSPLFFPGGDIGSLAVHGTVNDLAMGGATPLY
                     LTVGFILEEGLPLADLARIVRSLAQAAREAGVAVITGDTKVVEQGKGDGVFITTTGIG
                     VVPDGVNLSGHLAQPGDAVLVSGMLGDHGVAILSQREGLAFAADVVSDSAALHTLAAQ
                     LIAACPSLRLMRDPTRGGLATTLNEIAVQSGVGIRLDEAAIPVRPVVRAACEFLGLDP
                     LYCANEGKLTAICAAADAPALLAAMRAHPLGQDAAIIGEVIADPRCFVRARTPFGGER
                     LVDWLNGEMLPRIC"
     gene            complement(2099..>2917)
                     /locus_tag="PKN55_13195"
     CDS             complement(2099..>2917)
                     /locus_tag="PKN55_13195"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNX72248.1"
                     /translation="LVANVLTATLPGGRYLLPATLQPMLLAITHGEIRLAPADLLRPA
                     EACPALPVLGLCGATRSVREAWATPGTQILAVTVRTGQLPRLFGVSAQDVMEEFVPLD
                     TLAPPAWVASWAQRLRQAGTPAACRHALETGLCTQAARHMQRSPDLVLPQVCLDWPLA
                     RLAEHCQLGVRQFERQFLRAYGQSLRQFRQQWRCSQLLASQVFGMSSASKPRPWADIA
                     LDGAYFDQAHFSRDMRRFTGYSPAQLARQLAEQDPALWPYRFNSMDARRVLGME"
BASE COUNT          478 a          937 c         1012 g          490 t
ORIGIN      
        1 gccccatcca gccggccaat cggcagcaca cacaccggac agccggggcc gtggaacacg
       61 gcgtgaccct gtgcacctat ggcgacaccc tgcgcgtgcc cggttccggg cgcatgagct
      121 tgtacaaggc gcgcgcggct ggcggcgatg tgcgcatggt gtattcggtg gataacgcct
      181 tggccctggc gcgcgccgag ccgcagcgcc aggtggtgtt tttcgccatc ggctttgaaa
      241 ccaccacgcc gcctaccgcg ttggccttgc tgaccgcccg cgccgagggg ctgagcaatt
      301 tcagcgtgta cgccaaccat gtgctgaccc cggcggcgat gcgccatttg ctggccagcg
      361 gcgatggtgt ggcgctggat ggttttatcg ggccgtcgca cgtcagcacg gtgattggct
      421 cggatgccta cgccgacatt gcccgtgatt cgggcaagcc ggtggtgatt accggctttg
      481 agccgctgga cgtgctgatt ggcgtgcgca tgctgatcga gcagatcaac cagggccgcg
      541 ccgaggtgga aaaccagttt atccgcgcgg tgaccgccac cggcaaccgc aaggcgcggc
      601 aggtggtgga cgaagtgttt gccctgcgcc cggcgtttga ctggcgcggg ctgggcgagg
      661 tggccgccag cgcgctggcc atccgcccgg aatggtcggc atgggacgcc gaggtgaaat
      721 ttggcctcac cacccacagc gtggcggaca acaaagcctg tgaatgcggc gccattttgc
      781 aggggcgcaa aaagccacag gactgcaagg tgtttgccac ggtgtgtacc ccggacaacc
      841 cgctgggggc ctgtatggtg tccagcgaag gcgcctgcgc ggcgcactac gcctatggcc
      901 gttttgtcag caaggagccg gcatgagcat caaaccaggc tacacccgcc cgctggacct
      961 gaaacacggc cgggtggaca tgacccacgg cagcggcggc cgggcgatgg cgcagctgat
     1021 tcatgagctg tttgccgccg aatttggcaa tgaatggctg gcgcagggca acgaccaggc
     1081 gattgtgccg ccgccgggtg gccgcctggc ggtggccacc gatggccatg tggtgtcgcc
     1141 gctgtttttt cctggtggcg acatcggcag cctggcggtg cacggcacgg tcaacgacct
     1201 ggccatgggc ggcgccacgc cgctatacct gacggtgggt tttattctgg aagaaggcct
     1261 gccgctggct gatctggcgc gcattgtccg ctcgctggcg caggccgcgc gtgaggctgg
     1321 cgtggcggtg atcaccggcg acaccaaggt ggtcgagcag ggcaagggcg atggcgtgtt
     1381 tatcaccacc actggcattg gtgtggtgcc cgacggggtg aatctgtctg gccacctggc
     1441 gcagccgggt gacgcggtgc tggtgtctgg catgctgggc gaccatggcg tggcgattct
     1501 cagccagcgc gaaggcctgg cgtttgccgc cgacgtggtc agcgacagcg ccgcgctgca
     1561 caccctggcc gcccagttga ttgccgcctg cccgtcgctg cggctgatgc gcgaccccac
     1621 tcggggcggc ctggccacca cgctgaatga aatcgccgtg caatctggtg tgggcattcg
     1681 tctggacgaa gcggcgattc cggtgcgccc ggtggtgcgc gccgcctgcg agtttttggg
     1741 gctggatccg ctgtactgcg ccaacgaagg caagctcacc gccatctgcg ccgccgccga
     1801 cgccccggcg ctgctggccg ccatgcgcgc ccacccgctg ggccaggacg cggccatcat
     1861 cggcgaggtg attgccgacc cgcgctgctt tgtccgcgcc cgcaccccgt ttggcggcga
     1921 gcggctggtg gattggctga atggcgagat gctgccgcgg atttgctagg aatgagctga
     1981 agcccgatac gccagacaga taaaaccccc gccagcgtca gccagcgggg gttttgtcat
     2041 ctcatcaagc tgggagggtc tgcaatcaca cgcctttgcc gagttttgtg cgcggcgctt
     2101 actccatccc caacacccgc cgggcatcca tgctgttgaa acggtagggc cacagcgccg
     2161 gatcctgctc ggccagctgg cgagccagtt gcgccgggct gtagccggta aaccggcgca
     2221 tgtcgcggct gaagtgggct tgatcaaaat acgcgccatc cagcgcgata tcggcccagg
     2281 gccgtggctt gctggcggac gacatgccaa acacctgact ggccagcagc tgactgcaac
     2341 gccattgctg acgaaactgg cgcaggcttt ggccgtaggc gcgcaaaaac tgacgctcga
     2401 actgacgcac acccagctga caatgctcgg ccagcctggc cagcggccag tccaggcaaa
     2461 cctgcggcag caccaggtcc gggctgcgct gcatgtggcg ggcggcctgg gtgcacaagc
     2521 cggtttccag cgcatggcga caggcagccg gggtgccggc ctggcgcaga cgctgtgccc
     2581 aactggccac ccaggcaggc ggggccaggg tatccagcgg cacaaattct tccatcacat
     2641 cctgtgctga tacgccaaac agccgtggca gttggcctgt gcgcacggtc acggccagaa
     2701 tttgcgtgcc gggcgtggcc cacgcttcac gcacgctgcg ggtggcgcca cacagcccca
     2761 gcaccggcag ggccggacac gcctcggcag gccgtaacag atcggctggc gccaggcgga
     2821 tttcaccgtg ggtgatggcc agcagcatcg gttgcagcgt ggccggcaac agatagcgtc
     2881 cgccgggcaa ggtggcggtc agcacattgg ccaccag
//
LOCUS       DAOPYX010000349         2917 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_47164_length_2917_cov_5.980433, whole genome shotgun sequence.
ACCESSION   DAOPYX010000349 DAOPYX010000000
VERSION     DAOPYX010000349.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 2917)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2917)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2917
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47164_length_2917_cov_5.980433"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(109..414)
                     /gene="cas2"
                     /locus_tag="PKN55_13200"
     CDS             complement(109..414)
                     /gene="cas2"
                     /locus_tag="PKN55_13200"
                     /inference="COORDINATES: protein motif:HMM:TIGR01573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HNX72249.1"
                     /translation="MWLIIMFDLPTTDSEARADYRHFHDFLLNEGYLRLQLSVYGRHC
                     ASEENGAVHMQRLRAALPPDGEVRVLTLTDKQFSRMQVFFGRMRQRTEPPPEQILLL"
     gene            complement(405..1319)
                     /gene="cas1"
                     /locus_tag="PKN55_13205"
     CDS             complement(405..1319)
                     /gene="cas1"
                     /locus_tag="PKN55_13205"
                     /inference="COORDINATES: protein motif:HMM:TIGR03639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004520 - endodeoxyribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CRISPR-associated endonuclease Cas1"
                     /protein_id="HNX72250.1"
                     /translation="MIKRIIELTHGSYVHLHHHQLVIEQDGQCVASAPAEDVGLLLLA
                     HPANVITQQALACCQQYGVAIAVCDEHHLPSALMLPLDGHSLHAEILRQQVAASPAAQ
                     AALWQAVIRGKISAQAEVLAKLGRPGEARLRRLAEHVRPGDPDNLEAQAAAAYWRSLL
                     PEGEHRQPGRHTGVNALLDYGYAILRAAVARAVVAAGLHPAWGIFHHNRQDNFALADD
                     LMEPLRPAVDWLACRHIRDPSQGLSPGDKRALLAMLAAQVQLGDVRLPLWVALGRYIA
                     QAKQAWFGENLTVEIPKWLFLEDTEPCG"
     gene            complement(1316..>2917)
                     /gene="cas9"
                     /locus_tag="PKN55_13210"
     CDS             complement(1316..>2917)
                     /gene="cas9"
                     /locus_tag="PKN55_13210"
                     /inference="COORDINATES: protein motif:HMM:TIGR01865.1"
                     /note="Cas9, originally named Csn1, is the large,
                     multifunctional signature protein of type II CRISPR/Cas
                     systems. It is well known even to general audiences
                     because its RNA-guided endonuclease activity has made it a
                     popular tool for custom editing of eukaryotic genomes;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0004520 - endodeoxyribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CRISPR RNA-guided endonuclease Cas9"
                     /protein_id="HNX72251.1"
                     /translation="PDALLAEYGRPHAIRIELPRELAMGGKRKAEYEKQQKRNQKANE
                     DANTQYQTIRQANPQLGLAERVGRLEQLKYRLWRDQNERCLYSDKPISMTELFSAEVE
                     VDHILPYSRTVDDSYMNKALVMASENRFKGNRTPYEAYSGQPERWERMVQCARLQPPA
                     KRERLLRQELSGVDDFINNQLTDTAYLSRQVLAYVQLLGSAVDVSVSKGVLTAWLRRR
                     WGLNPLLGGGEKNRADHRHHAVDAAVIASVNRSLYQKLVKLMREPGARLERIALDAPF
                     AHYRDSLAAHLDRLCVSHAPLRKLSGALHEETVYAVQRGPQGEPRVVYRKPLDASLKL
                     DKLVDDGLRRQLQDHLARFNNHAKQAFAPENLPVLHPGHGPVRRVRVVAANSFNPRSF
                     LSLPDRQGQLRKHLPFGSNHHVEILRDRASGKVESVFVTMWEAAQRAHQHQQALVQTK
                     HGADKVFLMALHINDLVLIQQGERQVIYRVQNLDPKDKRVVLRHHRAATLDDDTQKVR
                     ASISQLVDQHGMQPLQLNVLGKPLP"
BASE COUNT          475 a          873 c          944 g          625 t
ORIGIN      
        1 cgtgccgacc cactagcggt tgtgaatgcc tcacaggcat cagtcagtta cactagaccc
       61 atgctatctt attgatggcg tgggtctttt tacttttgcc aatctgattt acaacagcag
      121 tatttgctct ggcggcggct cggtgcgctg gcgcatgcgg ccaaaaaaca cctgcatgcg
      181 ggaaaactgc ttgtcggtga gcgtcaacac gcgcacttca ccgtctggcg gtagggcggc
      241 gcgcagcctt tgcatgtgca ccgcgccgtt ttcttcgctg gcgcagtggc ggccgtacac
      301 tgagagttgc aggcgcagat aaccttcgtt cagcaaaaaa tcatgaaaat gccgatagtc
      361 ggcgcgcgcc tcgctatcgg tggtgggcag gtcaaacatg atgatcagcc acatggttcg
      421 gtatcctcca aaaacagcca tttcggtatc tccaccgtca ggttttcgcc aaaccaggct
      481 tgtttggcct gggcaatata gcgccccagc gccacccaca gcggcagacg cacatcgccc
      541 agctgcacct gggcggccag catggccagc agcgcgcgtt tgtcgccggg gctcagccct
      601 tggctggggt cgcggatgtg gcggcaggcc agccagtcta ctgccgggcg cagtggctcc
      661 atcaggtcgt cggccagggc gaaattatcc tggcggttgt gatgaaaaat cccccaggca
      721 gggtgcaggc cggccgccac caccgcgcgg gccacggctg cgcgcaggat ggcgtagccg
      781 tagtccagca aggcgttcac cccggtgtgg cggccaggct ggcggtgctc gccctctggc
      841 aacaggctgc gccagtatgc ggcggcagct tgcgcttcca gattgtcggg gtcgccgggg
      901 cgcacatgct cggccagtcg gcgcagacgc gcctcgcctg gccggcccag ctttgccagc
      961 acctcggcct gggcgctgat tttgccgcgt atcaccgctt gccacagcgc cgcctgggcg
     1021 gcggggctgg ccgccacttg ctggcgcaga atttcggcgt gcaggctgtg gccatccagc
     1081 ggcagcatca gcgcgctggg caggtggtgt tcgtcgcaca cggcaatggc gacgccatat
     1141 tgctggcagc aggccagcgc ctgttgggtg atgacattgg ccgggtgggc cagcaatagc
     1201 aggccgacat cttcggccgg cgcgctggcc acacactggc catcttgctc aatcaccagt
     1261 tgatgatgat gcagatgcac atagctgcca tgggtgagct cgataatgcg cttgatcatg
     1321 gcaagggctt gcccagcacg ttgagttgta gtggctgcat accatgttga tcgaccaatt
     1381 gactgatgct ggcgcgcact ttttgggtgt catcatccag cgtagcggcc cggtgatggc
     1441 gcaacacaac gcgcttgtct ttcggatcca gattttgcac ccgataaatc acttgccgtt
     1501 caccttgctg aatcagcacc agatcattga tgtgcagcgc catcaggaac actttgtcgg
     1561 cgccgtgctt ggtttgcacc agcgcttgct ggtgttgatg ggcgcgctgg gcggcctccc
     1621 acatggtgac aaatacgctt tccaccttgc cgctggcgcg gtcgcgcagg atttccacat
     1681 ggtgattgct gccaaagggc aagtgtttgc gcaattggcc ctggcggtct ggcaggctta
     1741 aaaagctgcg cgggttaaag ctgttggccg ccaccacgcg cacgcggcgc accggcccgt
     1801 ggccagggtg cagcacgggc aggttttctg gggcaaaggc ttgcttggcg tgattgttaa
     1861 agcgcgccag gtggtcttgc agctggcggc gcaggccgtc atccaccagt ttgtccagct
     1921 tcaggctggc gtccaatggt ttgcgataca ccacgcgcgg ctcgccttgc gggccgcgct
     1981 gtacggcata gacggtttct tcatgcagcg cgccgctgag tttgcgcagc ggcgcatggc
     2041 tgacgcacag gcggtccagg tgggcggcca ggctgtcgcg gtagtgggca aatggggcat
     2101 ccagcgcaat gcgctccagc cgggcgccgg gttcgcgcat cagcttgacc agtttttgat
     2161 acaggctgcg gttaacgctg gcaatcacgg cggcgtctac ggcgtggtgg cggtggtcgg
     2221 cgcggttttt ttctcccccg cccagcagcg ggttcagccc ccagcgccga cgcagccagg
     2281 cggtgagcac gcccttgctg accgacacat ccaccgcgct gcccagcagt tgcacatagg
     2341 ccagcacttg ccggctgagg taggcggtgt cggtcagctg gttattgata aagtcatcca
     2401 cccctgataa ttcttgccgc agcaggcgtt cgcgcttggc agggggttgc aggcgggcgc
     2461 attgcaccat gcgttcccag cgttcaggct ggccgctgta tgcctcgtaa ggcgtgcggt
     2521 tgcccttgaa gcggttttcg ctggccatca ccagggcttt gttcatatac gagtcgtcca
     2581 ccgtgcgcga atacggcagg atgtgatcca cttcgacttc ggcagaaaac aactcggtca
     2641 tgctgatggg tttgtctgaa tacaggcagc gttcgttttg gtcgcgccac aggcggtatt
     2701 tcagctgctc cagccggccc acgcgctcgg ccagccccag ttgcggattg gcctggcgga
     2761 tggtttggta ttgcgtgttg gcgtcttcgt tggctttttg gttgcgcttt tgttgttttt
     2821 cgtattccgc cttgcgcttg ccgcccatgg ccagttcgcg cggcagttcg atgcggatgg
     2881 cgtgcgggcg gccatattcg gccagcagcg cgtcggg
//
LOCUS       DAOPYX010000350         2914 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_47228_length_2914_cov_9.640434, whole genome shotgun sequence.
ACCESSION   DAOPYX010000350 DAOPYX010000000
VERSION     DAOPYX010000350.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 2914)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2914)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2914
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47228_length_2914_cov_9.640434"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            546..2855
                     /locus_tag="PKN55_13215"
     CDS             546..2855
                     /locus_tag="PKN55_13215"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNX72252.1"
                     /translation="MPAGKRFFPRVEQARWLLWLPAAVLIAFVLSLGGMFYVVFFDWQ
                     DERRDSLIQDILWLEQSMRSQLTEQQDWAVRTASEVGEGTLDEDRFQAASELILRENP
                     EILSVELLNAQGQVEARAPSQFQAEHRRQPLASFEERDAFDVARRQGQPAFSNVYYSL
                     DKQARVDLEAPVYRDHRFAGSVRLSYSLPGMLHHEVPWWIGQKYQIAIVDLGGKVIAA
                     KFTVGERPGSLWHEIDFTPPGNGLRLRATAYHLGMGLAFPLLAGLMGLLLLLLGVSLW
                     KIRQHIRRRHKVESMLREEMALRAAMEDSMKNGLIVMDPRGTIVRVNRAFCEMTGRTA
                     EALTGQTPPYTFWPTEHLDELHGFVSATLAGDMPPHGFELPFLRADGESFWVRLYATP
                     LINHLNQQTGWLASMYNITELKKKREAIAQAHQRFRTVLNGLDAAVCVSRCEDRQLLF
                     SNRAFEENMVRASEDAPFCVVLPWPDDEGIGPLADVVDCDLQFNGSTRWYQLHRRRIE
                     WVDGEPAWLGIYADITEARAFAERERLQAEKLQTTARLMTMGELASSLAHELNQPLAA
                     IASYAAGCLNRIEQTPALPAVQLATPLDKIARQARRAGEIIHGIRAFVKKREPKLARL
                     AVSELLEHTLTLAGPMLSQHRVAFHIECDDGLTLDADRVLLEQVLLNLVNNAVEAMRD
                     GKVARPTLTLTAHRQGERMRLSVSDNGPGLNPAVAEQLFTPFFTTKQEGMGIGLNICR
                     SIIEYHRGEFGHAPKPEGGCVFWLELPLN"
BASE COUNT          615 a          817 c          877 g          605 t
ORIGIN      
        1 tgtagcctct tgaaaagaag atgtcgcttt ttaaagcctg ccagagacaa gccacagctg
       61 gcaagcctct cgcggtaaaa agtcatgcgt tttgttcgct ttctgccaaa aagcacgatt
      121 actcagtata aatactggtt acaccacgcg ttctcagcac gagttttagg tgaatttacg
      181 ataagtattt ttgcgcatac tgggcaccta ggatagcacc acttgaaaga aagctgagtc
      241 accgataaaa agcattgaca ccaaaaaacc aatcaaaata aaagccctga tacatttcta
      301 taaagacaca cccttttatt caacaaaacg atagtttata aaaacgcacc ccagtaaaat
      361 aaaaccccgg actcttgcct tcgcgggaag aataattttg tcagcacatc ctgaaaccca
      421 gcgcgcttca ctgagttttg cgagtgcgat aaatgaacgg ccatggctat tgtgctcatt
      481 tgatctatcc ccacggcgtc cgacctggcc tacaatggcg ccattgcatg tgtgatgagg
      541 ccaagatgcc ggctggcaaa cgttttttcc cccgcgttga acaggcgcgc tggctgctgt
      601 ggctgccggc ggcggtattg attgcttttg tgctgtcgct gggcggcatg ttttatgtgg
      661 tgttttttga ttggcaggac gagcgccgcg atagcctgat tcaggacatt ctgtggctgg
      721 agcaatccat gcgctcgcag ttgaccgagc aacaggattg ggcggtgcgc accgccagtg
      781 aggtgggcga gggtacgctg gacgaagatc gttttcaggc cgccagtgaa ctgatcttgc
      841 gggaaaaccc ggaaattctt tctgttgaac tactcaatgc ccagggtcag gtagaagcac
      901 gcgcccccag ccagtttcag gccgagcacc gtcgccagcc gctggccagc tttgaagagc
      961 gcgatgcctt tgacgtggcg cgccgccagg gtcagccggc attcagcaat gtgtattaca
     1021 gcctggacaa gcaagctcgg gtcgatctgg aagcaccggt gtaccgtgat caccgctttg
     1081 ccggcagcgt gcgattgagc tacagcctgc cgggcatgct gcaccatgaa gtgccgtggt
     1141 ggattggcca gaaatatcaa atcgccattg tcgatttggg tggcaaggtg attgccgcca
     1201 agttcaccgt gggcgaacgg ccaggcagct tgtggcatga aatcgacttc accccgccag
     1261 gcaatggcct gcgcctgcgc gccaccgcct accacctggg catggggctg gcgtttccgc
     1321 tgctggccgg gctgatgggc ttgttattgc tgttgctggg ggtgagtttg tggaaaatcc
     1381 gccagcacat ccgccgccgg cataaggtgg aatccatgct gcgcgaagaa atggccttgc
     1441 gcgcggcgat ggaagactcg atgaaaaacg gcctgatcgt gatggatccg cgcggcacca
     1501 tcgtgcgggt caaccgggcg ttttgcgaga tgaccgggcg cacggccgag gcgctgactg
     1561 gccagacgcc gccttatacc ttctggccca ccgaacatct ggacgaattg cacggttttg
     1621 tttctgccac cctggccggc gacatgccac cgcacggttt tgaattgcct ttcctgcgcg
     1681 ccgacggaga atcattctgg gtgcggctgt acgccacgcc gctgatcaat cacctgaatc
     1741 agcaaaccgg ctggctggcg tcgatgtaca acatcaccga gctgaaaaaa aagcgcgaag
     1801 ccatcgccca ggcgcaccag cgctttcgca ccgtgctcaa cggcctggat gcggcggtgt
     1861 gcgtcagccg ctgtgaagac cgccagttgt tattctccaa tcgcgccttt gaagaaaaca
     1921 tggtgcgcgc cagcgaggac gcgccgttct gcgtggtgct gccgtggccg gacgatgaag
     1981 gtattggccc gttggccgat gtggtggact gtgatctgca attcaatggc agcacgcgct
     2041 ggtatcagct gcaccgccga cgcattgaat gggtggatgg cgaaccagcc tggctgggca
     2101 tttacgccga catcaccgag gcacgggcgt ttgccgagcg cgagcgtctg caagccgaaa
     2161 aactgcaaac cactgcccgg ctgatgacca tgggcgagct ggcctccagc ctggcgcacg
     2221 agctgaatca gccgctggcg gcgattgcca gctatgccgc cggctgcctg aaccgcattg
     2281 agcaaacccc ggcgctcccc gccgtgcaac tggccacccc gctggacaaa attgcccgcc
     2341 aggcgcgccg cgccggcgag atcatccacg gcattcgcgc ctttgtgaaa aaacgcgagc
     2401 ccaagctggc gcgactggcg gtcagcgagc tactggaaca cacgctgacg ctggccgggc
     2461 ccatgctgag ccagcatcgg gtggcgtttc atatcgaatg cgatgatggt ttgacgctgg
     2521 atgccgaccg ggtgttgctg gagcaggtgt tgttgaatct ggtgaataat gccgtcgagg
     2581 cgatgcgcga tggcaaggtg gcgcggccca cgctgacttt gacggcgcac cgccagggcg
     2641 agcgcatgcg gctgagcgtc agcgacaatg ggccgggcct gaatcctgcc gtggccgaac
     2701 agctgttcac gccgtttttc accaccaagc aagagggcat gggcattggg ttgaatatct
     2761 gccgctcgat cattgaatac catcgcggtg aattcggcca tgcccccaag ccggaggggg
     2821 gatgtgtgtt ttggttagag ttgccgctga attaagagcg gcaacgctca acccgcggcg
     2881 cagtggtctg cgccgatgag gcttattcga tgtt
//
LOCUS       DAOPYX010000351         2907 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_47386_length_2907_cov_11.710729, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000351 DAOPYX010000000
VERSION     DAOPYX010000351.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 2907)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2907)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2907
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47386_length_2907_cov_11.710729"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..392)
                     /locus_tag="PKN55_13220"
     CDS             complement(<1..392)
                     /locus_tag="PKN55_13220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012695603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HNX72253.1"
                     /translation="MALKATIFKADLNISDMDRGYYASHALTIARHPSEHDERMMLRI
                     AAFALHADEQLSFTKGLCADDEPEVWRKNYADEIELWIDLGEPDEKRLRKACGRADQV
                     WLYCYGGRASEIWWQGMQGKASRFSNLTV"
     gene            737..>2907
                     /locus_tag="PKN55_13225"
     CDS             737..>2907
                     /locus_tag="PKN55_13225"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HNX72254.1"
                     /translation="MSELKTLPIYRASPTPPASGDSCHGACSCGHPPATPPSPAAPPS
                     QLAAGHTRSVVRIAQMDCPVEANMIEHALGKLPQVSALAFNLLKRELTVDHAPGSEPH
                     WQAAIVALGFTVEAADAPAAPPPPERWLGLALSGAAALGAELAHAFAPWPWLSAVLAL
                     AAIAGCGLSTYRKGWLALRQRTLNINALMSVAVTGAVLIGQWPEAAMVMCLFVLAERI
                     EARSLARARDAISSLMAVTPDTAWVQTADGQWRQCPASELSAGSVLRVLPGERVALDA
                     VITAGASALDQSPITGESVPVDKTIGDTVFAGSLNTYGELTLRASAPASESTLARILH
                     AIEQAESRRAPTQRFVDQFARRYTPAVFALALLAAVAPPLLGWAGWLDSVYQALVMLV
                     IACPCALVISTPVTLVSGLAAAARHGVLVKGGAVLEQGARLRALALDKTGTLTLGQPR
                     LQQARALRGELADWRQAAGALAARSDHPVSRALALSEAAAWAFAGVRAWPGEGISGEQ
                     HGETYWLGNRRLAARFAAESSELDAALADWDAQGYTPVLFGCGAHTLALFAVADQLRP
                     AAPEAVAALAALGVHSAMLTGDGAAPAQAVAAQLSLNEVRAGLLPQDKLSEIEALQTR
                     HGVVGMVGDGINDGPALARADVGFAMAAAGSATALEVADVALMDDDPRKLAWFIRLAR
                     HTRAVLWQNIGLALGLKVVFLGLTLSGHGSLWLAVFADVGA"
BASE COUNT          449 a          968 c         1021 g          469 t
ORIGIN      
        1 acggtgaggt tgctaaaacg gctggccttg ccctgcatgc cttgccacca gatttcgctg
       61 gcgcgcccgc catagcaata caaccacacc tgatcggcgc ggccgcaggc cttgcgcagg
      121 cgcttttcgt cgggctcgcc cagatcaatc cacagctcga tttcatcggc gtagtttttg
      181 cgccacacct ccggctcgtc gtcggcgcac aagcccttgg tgaagctcag ctgttcgtcc
      241 gcatgcaaag caaatgcagc aatacgcaac atcatgcgct cgtcgtgctc agaaggatgg
      301 cgggcaatgg tcaaggcatg gctggcgtaa tagccgcgat ccatgtcgga gatattgaga
      361 tctgctttaa aaatggtggc tttcagcgcc atgggagcat ccgttgcaaa agtcggcaaa
      421 ggcgcgagca tactcgcctc gccagagggt gacaaaaccc acacaccaca gcaccggacg
      481 ctggcggcgc gttttgtgca tgaatggcaa gcaattgaat ctgcgcgctt gactctggag
      541 ttgctccaga gtgtttactg tcggcatgcc gcatttcgcg ccgctcacca aacccagcgc
      601 agcatgcctg gccagacctg caagcgcagc gggtgcgaga agcacggcag gtcgagccgg
      661 ttgggcgggc gccagccatc tggcacgcga aatgcacgaa caccgtgatg ttgtggccga
      721 cagaaaggga tgcgccatgt ccgagttaaa aaccctgccg atttaccgcg ccagcccgac
      781 gccgccagcc agtggcgaca gctgccatgg cgcgtgcagt tgcgggcatc cgcctgccac
      841 gccgcccagc cctgccgcgc cgcccagcca gttggcggcg ggccatacgc gcagcgtggt
      901 gcgcattgcc cagatggatt gtccggtaga agccaatatg atcgaacacg cgctgggcaa
      961 gctgccacag gtgtcggcgc tggcgtttaa tctgctcaag cgcgagctca ccgtcgatca
     1021 tgcgccgggc agcgagccac actggcaggc ggcgattgtc gcgctgggct ttaccgtcga
     1081 ggcggccgac gcgccggccg cgccgccgcc gcccgagcgc tggctggggt tggccttgtc
     1141 tggcgcggcg gcgctgggcg ccgagctggc gcacgccttt gccccctggc catggctgtc
     1201 ggccgtgctg gcgctggcgg cgattgccgg ctgtgggctg agcacctacc gcaaaggctg
     1261 gctggccttg cgccagcgca cgctgaacat caatgcgctg atgagcgtgg cggtgaccgg
     1321 cgcagtgttg atcggccagt ggccagaagc cgcgatggtg atgtgtttgt ttgtgctggc
     1381 cgagcgcatc gaggcgcgct cgctggcgcg cgcccgcgac gccatcagca gcctgatggc
     1441 ggtgacgccg gacaccgcct gggtgcaaac cgccgacggc caatggcggc aatgcccggc
     1501 cagcgagctg agcgccggca gcgtgctgcg cgtgctgccc ggcgaacggg tggcgctgga
     1561 tgccgtcatc accgccggcg cgtcggcgct ggaccaatcg ccaatcaccg gcgaaagcgt
     1621 gccggtggat aaaaccatcg gcgacacggt gtttgccggc agcctgaaca cctatggcga
     1681 actcaccctg cgcgccagcg cgccggccag cgaatccacc ctggcgcgca ttctgcacgc
     1741 catcgaacaa gccgaatcgc gccgcgcccc cacgcaacgc tttgtcgatc aatttgcccg
     1801 acgctacacc ccggcggtgt tcgccctggc cttgctggcc gccgtggccc cgccgctgct
     1861 gggctgggcc ggctggctgg acagcgtgta tcaggcgctg gtgatgctgg tgattgcctg
     1921 cccgtgcgcg ctggtgattt ccaccccggt caccctggtc agcggcctgg ccgccgccgc
     1981 gcggcatggc gtgctggtga agggcggcgc ggtgctggag caaggcgcgc ggctgcgcgc
     2041 gctggcgctg gacaaaaccg gcacgctgac gctgggccag ccgcgcctgc aacaggcgcg
     2101 cgcgctgcgc ggcgaactgg ccgactggcg tcaggctgct ggcgcgctgg cggcgcgctc
     2161 ggaccacccg gtgtcgcgcg ccctggcgtt gagcgaggct gccgcctggg cgtttgccgg
     2221 cgtgcgcgcc tggccgggcg agggcatcag cggtgaacag catggcgaaa cctactggct
     2281 gggcaatcgt cgcctggcgg cgcgctttgc tgcggaatcc agcgagctgg acgccgcgct
     2341 ggccgactgg gacgcgcaag gctacacccc ggtgctgttt ggctgcggag cgcacacgct
     2401 ggcgctgttt gccgtggccg accagctgcg ccccgccgcg cccgaggcgg tggccgcgct
     2461 ggcggcgctg ggcgtgcaca gcgccatgct gaccggcgac ggcgccgcgc ccgcgcaagc
     2521 ggtggcggcg cagctgtcgc tgaatgaagt gcgcgccggg ctgttgccgc aagacaagct
     2581 cagtgaaatc gaagccctgc aaacgcgcca cggcgtggtg ggcatggtgg gcgacggcat
     2641 caacgacggc ccggcgctgg cgcgcgccga cgtcggcttt gccatggccg ccgccggctc
     2701 ggccaccgcg ctggaagtgg ccgacgtggc gctgatggac gacgacccgc gcaagctggc
     2761 ctggtttatc cgcctggcgc ggcacacccg cgccgtgctg tggcagaaca tcggcctggc
     2821 gctggggctg aaagtggtgt ttttaggctt gacgctcagc ggccatggca gcctgtggct
     2881 ggcggtgttc gccgatgtcg gcgccag
//
LOCUS       DAOPYX010000352         2897 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_47650_length_2897_cov_8.359958, whole genome shotgun sequence.
ACCESSION   DAOPYX010000352 DAOPYX010000000
VERSION     DAOPYX010000352.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 2897)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2897)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2897
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47650_length_2897_cov_8.359958"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..307)
                     /locus_tag="PKN55_13230"
     CDS             complement(<1..307)
                     /locus_tag="PKN55_13230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012696582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNX72255.1"
                     /translation="MATPLLHIDQLTVRFGAAEVVRQVSLDIHPGEKLALVGESGSGK
                     TVMAQAVLRLNREVNYQGRIELDGVGDVLSLSERALRGVRGRDAAMIFQEPMTALNPL
                     "
     gene            complement(410..664)
                     /gene="infA"
                     /locus_tag="PKN55_13235"
     CDS             complement(410..664)
                     /gene="infA"
                     /locus_tag="PKN55_13235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017185218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HNX72256.1"
                     /translation="MAKEEPIEFEGRVSELLPNSTFLVKLDNGAEVIAYASGKMRKHR
                     IRILAGDRVTVEMSPYDLSKARVSFRHKDTNPRPTPAPSR"
     gene            complement(799..874)
                     /locus_tag="PKN55_13240"
     tRNA            complement(799..874)
                     /locus_tag="PKN55_13240"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(839..841),aa:Met,seq:cat)
     gene            1242..2648
                     /gene="leuC"
                     /locus_tag="PKN55_13245"
     CDS             1242..2648
                     /gene="leuC"
                     /locus_tag="PKN55_13245"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014086692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HNX72257.1"
                     /translation="MTAQTLYDKLWQSHVVHEEADGTVLLYIDRHLIHEVTSPQAFEG
                     LKLAGRQPWRVGSIVATADHNTPTDNWDKGIQDPISRQQVETLDANIREVGARAYFPF
                     KDQGQGVIHVMGPEQGATLPGMTVVCGDSHTSTHGALGCLAHGIGTSEVEHVLATQTL
                     VAKKSKSMLISVEGQLGAGVSAKDVALAIIGKIGTAGGTGYAIEFAGSAIRGLTVEGR
                     MTLCNMAIEAGARAGMVAVDQATIDYVQGRPLAPTGAMWDQAVAYWKTLHSDEGAQFD
                     AVVELKAEDILPQVTWGTSPEMVVTVADRVPDPAQEADPVKKSGIERALSYMGLTANT
                     PVNEIPVDVVFIGSCTNSRIEDLRAAASVIRGRQKAANVKRVLVVPGSGLVKAQAEAE
                     GLDRLFVEAGFEWREPGCSMCLAMNADRLEPGERCASTSNRNFEGRQGQGGRTHLVSP
                     AMAAAAALAGHFVDVRSF"
     gene            2663..>2897
                     /locus_tag="PKN55_13250"
     CDS             2663..>2897
                     /locus_tag="PKN55_13250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HNX72258.1"
                     /translation="MKAFTTLNGLVCPLDRANVDTDAIIPKQFLKSIKRSGFGPNLFD
                     EWRYLDHGEPGMDNSTRPVNPDFVLNFPRYQGAQ"
BASE COUNT          575 a          913 c          907 g          502 t
ORIGIN      
        1 acaatgggtt cagcgcggtc atcggctctt gaaaaatcat cgccgcgtcg cgcccacgca
       61 cgccgcgcag ggcgcgctcg gacaggctga gcacgtcgcc gacgccgtcc agttcgatgc
      121 gcccctggta gtttacttcg cggttcagcc gcagcacggc ttgcgccatc accgtcttgc
      181 ccgagccgga ttcgcccacc agggcgagtt tttcgccggg gtggatatcc agcgagactt
      241 ggcgcaccac ttcggcagcg ccaaagcgga cggtgagctg atcaatgtgc agcaaggggg
      301 tggccatcgg ggcgaggtcc tgaggatgac gattcagacc tgcgtagtat accggccgtg
      361 ttgcacggac ggtgaggggc gtggccagaa acccgccccg tgggctgctt tagcggctgg
      421 gggccggtgt cggacgcggg ttggtgtctt tgtggcggaa gctcacccgt gccttgctca
      481 ggtcgtaggg ggacatttcc acggtgacgc ggtcgcccgc cagaatgcgg atgcgatgct
      541 tgcgcatttt gcccgaggcg taggcaatca cttcagcgcc gttgtccagc ttgaccagaa
      601 aggtggagtt gggcagcagc tcgctgacgc ggccttcaaa ttcaatcggt tcttctttgg
      661 ccatggccag gctcccaggc cgcgccgggg cgacccacgg tgcgcgccag accatgcagg
      721 gcttacgcgc gaatcaatag aacaccgtat ccggtttcgg cacagcacat gcaatgcagg
      781 aaaaaggagg cggaaaactg gttgcgggaa caggactcga acctgtgacc ttcgggttat
      841 gagcccgacg agctgccaac tgctccatcc cgcgtctgaa aggcaggcgc cgcccctcgc
      901 aagaacgagg gcaacgccag aataaactgg tgcagggact tcaagcactc cccgcctgat
      961 gcttcatgac tgatgccagg gcgccgcgcc gtaacactac ggggcgccag atcgggcggc
     1021 cggtcatccg ctgtacaaac gaaaatgaac gaccaaaaca accaacgaac cgccaatata
     1081 acagcacatc aggaataagt cgagtattta tttttatttg gttataaaac ccatccagcc
     1141 atcgtgattg gcgatggctg cgtctgttat tttcagttcc gttcaggtcg gggcggcggc
     1201 ataattcggg ctgaattttc ccgcacaagc gagccgcaac catgaccgca caaacgcttt
     1261 acgacaaact ctggcaaagc catgtggtgc acgaagaagc cgacggcacc gtgctcttgt
     1321 acatcgaccg ccacctgatc cacgaagtga ccagcccgca agcctttgaa ggcctgaagc
     1381 tggccggccg ccagccgtgg cgcgtgggct cgattgtcgc caccgccgac cacaacaccc
     1441 ccaccgacaa ctgggacaag ggcattcagg acccgatttc ccgccagcag gtggaaaccc
     1501 tggacgccaa tatccgcgaa gtgggcgcgc gcgcttactt tccgttcaag gatcaaggcc
     1561 agggcgtgat tcacgtcatg ggcccggaac aaggcgccac cctgccgggc atgaccgtgg
     1621 tgtgcggcga cagccacacc tccacccatg gcgcgctggg ctgtctggcg cacggcatcg
     1681 gcacctcgga agtcgagcat gtgctggcca cgcaaaccct ggtggcaaaa aaatccaagt
     1741 ccatgctgat ttcggtggaa ggccagctgg gcgcgggcgt gtcggccaag gatgtggcgc
     1801 tggccatcat cggcaaaatc ggcaccgctg gcggcactgg ctacgccatt gagtttgccg
     1861 gcagcgccat tcgcggcctg acggtggaag gccgcatgac cttgtgcaat atggccatcg
     1921 aagctggcgc ccgcgctggc atggtggcgg tggaccaagc caccatcgac tacgtgcaag
     1981 gccgtccgct ggcgccgacc ggcgcgatgt gggatcaagc cgtggcctac tggaaaaccc
     2041 tgcactcgga cgaaggcgcg cagtttgacg ccgtggttga gctcaaggcc gaagacatcc
     2101 tgccgcaagt cacctggggc acctcgcccg aaatggtggt caccgtggcc gaccgcgtgc
     2161 ccgatccggc gcaagaagcc gatccggtga aaaaatccgg catcgagcgc gcgctcagct
     2221 atatgggcct gaccgccaac accccggtga atgaaatccc ggtggacgtg gtgtttatcg
     2281 gctcgtgcac caactcgcgc atcgaagacc tgcgcgccgc cgccagcgtg attcgcggcc
     2341 gccaaaaagc cgccaacgtc aagcgcgtgc tggtggtgcc gggctcgggc ctggtcaagg
     2401 cgcaagccga ggccgaaggg ctggaccgcc tgtttgtcga agccggcttt gaatggcgcg
     2461 agccgggctg ttccatgtgt ctggcgatga acgccgaccg cctggagccg ggcgagcgct
     2521 gcgcgtccac ctccaaccgc aactttgaag gccgtcaggg ccagggcggg cgcacccatc
     2581 tggtcagccc ggccatggcc gccgccgccg cgctggctgg ccactttgtt gacgtgcgca
     2641 gtttctgaca ggagcccacc ccatgaaagc cttcaccact cttaatggcc tggtgtgccc
     2701 gctggatcgc gccaatgtgg acaccgacgc cattatcccc aagcaatttt tgaagtcgat
     2761 caagcgttcc ggctttggcc ccaatctgtt tgacgaatgg cgctacctgg accacggcga
     2821 accgggcatg gacaacagca cccgcccggt gaacccggac tttgtgctga acttcccgcg
     2881 ctatcagggc gcgcaag
//
LOCUS       DAOPYX010000353         2895 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_47705_length_2895_cov_8.413380, whole genome shotgun sequence.
ACCESSION   DAOPYX010000353 DAOPYX010000000
VERSION     DAOPYX010000353.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 2895)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2895)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2895
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47705_length_2895_cov_8.413380"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..689)
                     /gene="cybH"
                     /locus_tag="PKN55_13255"
     CDS             complement(<1..689)
                     /gene="cybH"
                     /locus_tag="PKN55_13255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010646699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase, b-type cytochrome subunit"
                     /protein_id="HNX72259.1"
                     /translation="MKTYGFDGDAPQLSGQRKVTSVYVYEAPLRLWHWITVLSIIVLS
                     VTGYFIGKPLPSVPGEATFNYVMGYIRFAHFTAGYILAVGLLGRVYWAFVGNHHAREI
                     ILVPVWDKQWWKEFFFEIRWYLFLERYPKKYIGHNPVGQIAMASFLWITLFMCVTGFA
                     LYGEGTGAGSWAHSAFGWVIGAFGSSLDVHNWHRLAMWGIVLFVMVHVYAAIREDIMS
                     KQSMISTMVSG"
     gene            complement(704..2497)
                     /locus_tag="PKN55_13260"
     CDS             complement(704..2497)
                     /locus_tag="PKN55_13260"
                     /inference="COORDINATES: protein motif:HMM:NF012592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent hydrogenase large subunit"
                     /protein_id="HNX72260.1"
                     /translation="MSSYTTQGFSLDNSGRRVVVDPVTRIEGHMRCEVNLNSQNVITN
                     AVSTGTMWRGLEVILKGRDPRDAWAFVQRICGVCTGTHALTSVRAVENALGIAIPKNA
                     NLIRNLMQATLYVHDHLVHFYHLHALDWVDVVSALKADPKATSALAQSISSWPNSSPG
                     YFHDLQMRLKKFVESGQLGPFRNGYWGHPAYKLPPEANLMAVAHYLEALDFQKDIVKV
                     HAIFGGKNPHPNWLVGGMPCAINIDDVGAVGAINMERLNLVKGIIDRSIAFCEQVYLP
                     DLTAIAGFYKDWSKIGGGLASQSLLSYGDFPSIANDYSEKSLLLPRGAIINGKLTEVH
                     PVDLTAPDEIQEFVTHSWYTYGDDSKGLHPWDGRTEPKFELGKGHKGTKTKIEQLDES
                     AKYSWIKSPRWKGHAMEVGPLARYVIGYMQNKPEFKEPVEALLKKLDAPVEALFSTLG
                     RTAARGLESVWAAYEMRRNYDELIANIKAGDTATANIEKWEPASWPKQVKGVGFTEAP
                     RGALGHWLKIDNTRIDSYQCIVPTTWNAGPRDSKGQIGAYEASLMNTQLAKPDEPLEI
                     LRTLHSFDPCLACSTHVMSPEGEELISVAVR"
     gene            complement(2497..>2895)
                     /locus_tag="PKN55_13265"
     CDS             complement(2497..>2895)
                     /locus_tag="PKN55_13265"
                     /inference="COORDINATES: protein motif:HMM:NF026070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX72261.1"
                     /translation="RPHFDAGQFVEHWDDEGARKGYCLYKVGCKGPTTYNACSTVRWN
                     EGVSWPVQSGHGCLGCSEDGFWDKGSFYDRVTGIPQFGIEANADAIGLTAAGGVGAAI
                     AAHAAVSALKSTALKKQDLKPVETTQAEDK"
BASE COUNT          519 a          890 c          898 g          588 t
ORIGIN      
        1 aaaccgctga ccatggtgga gatcatgctc tgcttggaca tgatgtcctc gcggatggcg
       61 gcgtacacat gcaccatcac aaacagcaca atgccccaca tcgccagccg gtgccagtta
      121 tgcacgtcca ggctgctgcc aaaggcgcca atcacccagc caaaggcgct gtgcgcccag
      181 ctgccggcgc cggtgccttc gccgtacagg gcaaagccgg tcacgcacat gaacagggtg
      241 atccacaaaa agctggccat ggcgatctgg cccaccgggt tgtggccgat gtattttttc
      301 gggtagcgct ccaggaacaa ataccagcgg atttcaaaga aaaactcttt ccaccactgt
      361 ttatcccaca ccggcaccag gatgatttcc cgcgcgtggt ggttgcccac aaacgcccag
      421 tacacccggc caagcaggcc caccgccaga atgtagccgg cggtgaaatg ggcaaagcgg
      481 atataaccca tcacatagtt gaaggttgcc tcaccgggca cgctgggcag cggcttgccg
      541 atgaaataac cagtgaccga cagcacgatg atgctcagca cggtgatcca gtgccacagc
      601 cgcaacggcg cctcgtagac atacaccgag gtgaccttgc gctggccgga cagctgcggg
      661 gcgtcgccgt caaaaccata ggttttcatg gggaatcctc cgcttagcgc acggcaacgc
      721 tgatcagctc ttcaccttcc gggctcatca catgcgtcga gcaggccagg caggggtcaa
      781 agctgtgcag ggtgcgcagg atttccagcg gctcgtccgg cttggccagc tgggtgttca
      841 tcaggctggc ttcgtaagcg ccgatctggc ccttgctgtc acgcgggccg gcgttccaag
      901 tggtgggcac gatgcactgg tagctgtcga tgcgggtgtt gtcgattttc agccagtggc
      961 ccagcgcgcc gcgcggcgct tcggtaaagc ccacgccctt gacctgcttg ggccaggagg
     1021 ccggctccca tttttcgata ttggcggtgg cggtgtcgcc ggccttgatg ttggcaatca
     1081 gctcgtcgta attgcggcgc atctcgtagg cggcccacac gctttccagg ccacgggcgg
     1141 cggtgcggcc cagggtggag aacagtgctt ccaccggggc gtccagcttt ttcagcagcg
     1201 cttccaccgg ttctttgaat tccggcttgt tctgcatata gccaatcacg tagcgggcca
     1261 gcgggcccac ttccatggcg tggcccttcc agcgcggcga cttgatccag gaatacttgg
     1321 ccgattcgtc cagctgttcg atcttggtct tggtgccctt gtggcccttg cccagctcga
     1381 atttcggctc ggtgcggcca tcccacgggt gcaggccctt gctgtcgtcg ccgtaggtgt
     1441 accaggagtg ggtgacgaac tcctgaattt cgtccggcgc ggtgaggtcc accgggtgca
     1501 cttcggtcag cttgccattg atgatggcgc cgcgcggcaa cagcaggctt ttttcgctgt
     1561 aatcgttggc gatgctgggg aagtcgccat aagacagcag gctctggctg gccaggccgc
     1621 cgccgatctt ggaccaatcc ttgtaaaagc cagcaatcgc ggtcaggtcg ggcaggtaaa
     1681 cctgctcgca gaaggcaatc gagcggtcga tgatgccctt gaccaggttc agccgctcca
     1741 tattgatggc gccgaccgcg ccgacgtcgt caatattgat cgcgcacggc atgccgccca
     1801 ccagccagtt cggatgcggg tttttgccgc caaaaatggc gtgtaccttg acgatgtctt
     1861 tctggaaatc cagcgcttcc aggtagtgcg ccaccgccat caggttggct tccggcggca
     1921 gcttgtaggc cgggtggccc cagtagccat tgcggaacgg gccaagctgg ccggattcga
     1981 caaacttctt cagccgcatt tgcaggtcat gaaaataacc cggactggag ttgggccagc
     2041 tggagatgct ttgcgccagc gcgctggtgg ctttcgggtc ggctttcagc gccgacacca
     2101 cgtccaccca gtccagcgcg tgcaggtggt aaaagtgcac cagatggtcg tgcacataca
     2161 gcgtggcctg catcaggttg cggatcaggt tggcgttttt cgggatggcg atgcccaggg
     2221 cgttttccac cgcgcgcacc gaggtcagcg cgtgggtgcc ggtgcacacg ccgcagatgc
     2281 gctggacaaa ggcccaggca tcgcgcgggt cgcggccctt gagaatcact tccagaccgc
     2341 gccacatggt gccggtggac accgcattgg tgatgacgtt ctggctgttc aggttcacct
     2401 cgcagcgcat atggccttcg atgcgggtga ccgggtccac caccacgcgg cggccgctat
     2461 tgtccagcga gaacccttgt gttgtgtaac tgctcattat ttatcctccg cctgggtggt
     2521 ttcgaccggt ttgaggtcct gttttttcag ggctgtgctc ttgagcgcgc tgacagcggc
     2581 atgcgcggca atggcggcgc ccaccccgcc ggcggcggtc aggccgatgg cgtcggcgtt
     2641 ggcttcgatg ccgaattgcg gaatgccggt cacccggtcg tagaacgaac ccttgtccca
     2701 gaagccgtct tccgaacagc ccaggcagcc atggcccgat tgcaccggcc aggacacgcc
     2761 ctcgttccag cgcacggtgg aacaggcgtt gtaagtggtt gggcccttgc agcccacttt
     2821 gtacaggcag taacccttgc gcgcgccttc gtcgtcccag tgttcgacaa actggccggc
     2881 gtcaaaatgc gggcg
//
LOCUS       DAOPYX010000354         2804 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_50167_length_2804_cov_8.684613, whole genome shotgun sequence.
ACCESSION   DAOPYX010000354 DAOPYX010000000
VERSION     DAOPYX010000354.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 2804)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2804)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2804
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50167_length_2804_cov_8.684613"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..248)
                     /locus_tag="PKN55_13270"
     CDS             complement(<1..248)
                     /locus_tag="PKN55_13270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX72262.1"
                     /translation="MTSPRLTPAAIRAAHLPDDDALDPVAWGEVVSKMDEVFRELVAS
                     QEALEAKHAELAEAHDALQRTQAQLVQAEKMASLGRLVA"
     gene            complement(257..1678)
                     /locus_tag="PKN55_13275"
     CDS             complement(257..1678)
                     /locus_tag="PKN55_13275"
                     /inference="COORDINATES: protein motif:HMM:NF012592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent hydrogenase large subunit"
                     /protein_id="HNX72263.1"
                     /translation="MSRKILGPFNRVEGDLAVNLTLENGRVAQAQVTSPLYRGFERAL
                     VGRPLADALVVVPRICGICSVSQSVAAVRLMQALSGARPAANGERVMNIVHACENLAD
                     HLTHFYLFFAPDLAHADYAGQPWHADWAARLASGGPAARQWLAARATLLRIVGLLAGR
                     WPHTLALQAGGVTRTVDAAERLRLLGWLAEVRRFVETTLLGLPIDAMLAIDHPDQLER
                     LRAAGGDSGRWLRAADALGFAQLGRAHDHFLAFAAYGCDGEHPWPAGVWRDGAAQALN
                     LRRIQEDVSHSWYENAPARHPAEGDTRPNPHKHGAYSWNKAPRIDGLPAETGALARQL
                     LAGQGCLRALVAAHGGSVLTRVLARQFEAAWLLAAIERWLRELDLAAPFIAAAPHDAV
                     DGCAAGLTEAARGALGHWATVEHGQITRYQIIAPTSWNFSPRDAHGMPGPLETALQGV
                     ADHELSVQHVVRSFDPCMVCTVH"
     gene            complement(1675..2664)
                     /locus_tag="PKN55_13280"
     CDS             complement(1675..2664)
                     /locus_tag="PKN55_13280"
                     /inference="COORDINATES: protein motif:HMM:NF013242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX72264.1"
                     /translation="MKTLVWLGAGGCGGCSMSLLTAEAPDCLTALELAGIELLWHPSL
                     SALGGAQLLAMLDAMEAGAQTLDILCVEGALLTAFAGQCQTLGGGEPMAARVGRLARV
                     ARHVVAVGGCAAFGGVGAADDAGIGGRGLQFDGETPGGLLGASFVAASGLPVVNVAGC
                     PTHPGWVLDTLALLAADALPASGLDSLQRPRFYADRLVHHGCPRNEFYEFKATAHALG
                     QQGCLIEHLGCRGTLAHADCNIRPWHGASSCLKGGFSCIDCTAPDFAAAHRQPLTTPR
                     LAGLPTILPSDMPKAWFVALSALSKSATPARLRHNAQSERLDIAPTPPTGKRR"
BASE COUNT          435 a          948 c         1013 g          408 t
ORIGIN      
        1 gccaccagcc ggcccagcga ggccattttt tctgcctgca ccagctgcgc ctgggtgcgc
       61 tggagcgcgt cgtgcgcctc ggccagctcg gcgtgcttgg cctccagcgc ctcttgcgag
      121 gccaccagct cacgaaacac ttcgtccatc ttgctcacta cttcgcccca ggccaccggg
      181 tcgagggcgt cgtcgtcagg caaatgggcg gcgcggatgg cagccggggt caggcggggc
      241 gaggtcatgg aggttcctaa tgcaccgtgc acaccatgca cgggtcaaac gagcgcacca
      301 cgtgctgcac gctcaattcg tggtcggcca cgccttgcag cgcggtttcc agcgggccgg
      361 gcatgccgtg ggcgtcgcgc ggcgagaagt tccagctggt gggggcgatg atctggtagc
      421 gggtgatctg gccgtgttcc accgtggccc agtggcccag cgcgccgcgc gcggcttcgg
      481 tcaggccggc ggcgcagccg tccaccgcgt cgtgcggcgc ggcggcgata aagggcgcgg
      541 ccagatccag ttcgcgcagc cagcgctcga tggcggccag cagccaggcg gcttcaaact
      601 ggcgggccag cacgcgggtg agcaccgagc cgccgtgggc ggccaccagc gcgcgcaagc
      661 agccttgccc ggccagcagc tggcgcgcca gcgcgccggt ttcggcgggc aggccgtcga
      721 tgcgcggggc tttgttccag ctgtaggcgc cgtgcttgtg cgggttgggg cgggtgtcgc
      781 cttcggccgg gtggcgggcg ggggcgtttt cgtaccagct gtggctgacg tcttcttgaa
      841 tgcggcgcag attcagcgcc tgcgccgcgc cgtcgcgcca cacgccggcc ggccaggggt
      901 gttcgccgtc gcagccgtag gcggcaaacg ccaaaaaatg atcgtgcgcg cggccaagtt
      961 gggcaaaacc cagcgcgtcg gcggcgcgca gccagcggcc gctgtcgccg ccagcggcgc
     1021 gcagacgctc cagttggtcg gggtggtcga tggccagcat ggcgtcgatc ggcaggccca
     1081 gcagggtagt ttcgacaaag cggcgcactt cggccagcca gcccaataag cgcagccgct
     1141 cggcggcgtc caccgtgcgg gtgacgccgc cggcttgcag cgccagggtg tgcggccagc
     1201 gcccggccaa taggccgacg atgcgcagca gggtggcgcg cgccgccagc cattgccgcg
     1261 ccgctgggcc gccgctggcc agccgcgccg cccagtcggc atgccagggc tggccggcgt
     1321 agtcggcgtg ggccaggtct ggggcaaaaa acagataaaa atgggtgaga tggtcggcca
     1381 ggttttcgca ggcgtggacg atgttcatca cccgctcgcc attggccgcc gggcgcgcgc
     1441 cgctgagcgc ctgcatcagc cgcaccgccg ccaccgattg cgacaccgag caaatgccgc
     1501 aaatgcgcgg aaccaccacc agcgcatcgg ccagcggccg gccaaccagc gcgcgctcaa
     1561 agccgcgata tagcgggctg gtgacctgcg cctgggcgac gcggccgttt tccagggtga
     1621 ggttcaccgc cagatcgcct tccacgcgat tgaacgggcc cagaattttg cggctcatcg
     1681 gcgttttcct gtgggcgggg tgggggcgat gtccagccgt tctgactggg cgttgtggcg
     1741 cagccgcgcc ggggtggccg atttggacaa ggccgacagg gcgacaaacc aggctttggg
     1801 catgtccgac ggcaaaatgg tcggcaagcc ggccagccgg ggcgtggtca gcggctggcg
     1861 gtgggcggcg gcaaagtctg gcgcggtgca gtcgatgcag gaaaacccgc ccttcaggca
     1921 agagctggcg ccatgccagg ggcggatatt gcagtcggcg tgcgccagcg tgccgcgaca
     1981 gcccaggtgt tcgatcaaac agccttgttg gcccagcgcg tgggcggtgg ccttgaattc
     2041 gtaaaattca ttgcgcgggc agccgtgatg caccagccgg tcggcgtaaa agcgcgggcg
     2101 ctgcaagctg tccagcccgc tggccggcag cgcgtcggcg gccagcaagg ccagggtgtc
     2161 cagcacccag ccggggtggg tggggcagcc ggccacattc accaccggca ggccgctggc
     2221 ggcgacaaaa ctggcgccca gcaaaccgcc gggggtttcg ccgtcaaatt gcaggccgcg
     2281 cccgccaatg ccggcgtcgt cagcggcgcc aacgccgcca aacgcggcgc agccgcccac
     2341 cgccaccaca tggcgcgcca cccgcgccag ccggcccact cgcgccgcca tcggctcgcc
     2401 gccgcccagc gtctggcact ggccggcaaa ggcggtgagc agcgcgcctt ccacgcacag
     2461 gatgtccagt gtttgcgcgc cggcttccat ggcgtccagc atggccaaca gctgggcgcc
     2521 gcccagcgcc gacagtgagg ggtgccacaa gagttcaatg ccggccagct ccagcgcggt
     2581 taaacaatct ggcgcttcgg cagtcagcag cgacatcgag cagccgccac agccgcccgc
     2641 gcccagccag accagggttt tcattggcgt tcgccggatt caagtgataa ccggcaaatt
     2701 ataccgggat gcgcgcgccg gccgcagatc gggtgaagtt tattccgcca tgcggaattt
     2761 aagcccagga ttttgccgct ggcggttggc gcggcgattg gccg
//
LOCUS       DAOPYX010000355         2793 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_50482_length_2793_cov_9.951059, whole genome shotgun sequence.
ACCESSION   DAOPYX010000355 DAOPYX010000000
VERSION     DAOPYX010000355.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 2793)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2793)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2793
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50482_length_2793_cov_9.951059"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(168..1784)
                     /locus_tag="PKN55_13285"
     CDS             complement(168..1784)
                     /locus_tag="PKN55_13285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012697443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine--lipid A transferase"
                     /protein_id="HNX72265.1"
                     /translation="MKLLRPLSPERLALYVAMALVLLFNLPFWQRLWEIVSPLDGHGV
                     KMLALALALTTTFFYWVLLLFVWPRVGKPLIGLLLLTTAAVSYFMNQYGVLIDQDMVR
                     NALQTDGAEVRDLLTVKMALTVLLLGVLPCWLLWKLPVEYRPGWRRLGRRLAHLGIAT
                     LALAAIAAVGYQDFASLFRNHRELRMALAPLNYLQASTSYLRKVTAKPQPLQQIGLDA
                     KQTAAAHAKPRVLVLVVGETARADHFALNGYPRPTTPELAARTDIVNYPNAWSCGTET
                     AVSVPCMFSAKAREQFDTDRARHEENLLDVLKRAGLTVLWVDNNSGCKEVCNRVEKQM
                     RYDEQHAKYCRDGECFDEILLDGLPERLAALKGDAVIVLHQKGSHGPAYYKRYPPAFE
                     RFKPVCRTSELQQCAREDIINAFDNTIAYTDFFLAQTIRLLEKQANIDSSLIYLSDHG
                     ESLGENGMYLHATPYLVAPDAQKHIPMVAWFSPGWREHGGPALNCLKTQAGQRYSQDN
                     LFHSVLGMMGVSTALYQRELDMFAGCRQAS"
     gene            complement(1984..>2793)
                     /locus_tag="PKN55_13290"
     CDS             complement(1984..>2793)
                     /locus_tag="PKN55_13290"
                     /inference="COORDINATES: protein motif:HMM:NF014375.2"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HNX72266.1"
                     /translation="GFPEPARDAQGDRVYPREQVDKLRAVRRLMDQGFRPGKIIHLSA
                     EELSRLFEARRPREAAPAVCARLESLLKGQEGHAIRDELQCQLHSQGLRSFVCDFLPP
                     ANQLVGELWARGQIEIHEEHLYTEQVTRILRETLAVLTIPQGHPKVMLTTCAGEQHVL
                     GLLMAEALFRLSACEVLAFGADLPLTDIAQAAHKHRVDVLALSFSQAYDGAPPDTLTA
                     LRRLLPPQVELWVGGAGVQGMRPSPAGHWQILRNLHDIEPLLQDWRQRHLD"
BASE COUNT          538 a          884 c          891 g          480 t
ORIGIN      
        1 ccagcccgcc gttgcgacgc ctgctgacgc cgcaacggcc acgccaggcc gcagactgag
       61 cggctcttag agtcgctcac aaaacccagc gcagcgcgcc tggctaggcg cacgaccgta
      121 gacagtacaa cagggcaaca acgccaggag ggttttgtga gcgacgctta actggcctga
      181 cggcagccgg caaacatatc cagctcacgc tgatacagcg cggtgctcac ccccatcatc
      241 cccagcacgc tgtgaaacag attgtcctgg ctgtagcgct ggccggcctg ggttttcagg
      301 cagttcagcg ccgggccgcc gtgttcgcgc cagccgggcg agaaccaggc caccatcggg
      361 atgtgttttt gcgcgtccgg ggccaccaga tacggggtgg cgtgcagata catgccgttt
      421 tcacccagcg attcgccatg gtcggacaga taaatcaggc tgctgtcgat attggcctgc
      481 ttttccagca ggcggatggt ttgcgccaaa aagaaatcgg tgtaggcgat ggtgttgtcg
      541 aaggcgttga tgatgtcttc gcgcgcgcat tgttgcagct cgctggtgcg gcacaccggc
      601 ttgaagcgct cgaacgccgg cggatagcgc ttgtaataag ccgggccgtg gctgcctttt
      661 tggtgcagca caatcactgc gtcgcccttg agcgccgcca gccgctcggg caggccgtcg
      721 agcaggattt cgtcaaagca ttcgccatcg cggcagtatt tggcgtgctg ctcgtcgtag
      781 cgcatctgtt tttctacccg gttgcagact tctttgcagc cggaattatt gtccacccac
      841 agcacggtga ggccggcgcg cttgagcacg tccagcaggt tttcttcgtg gcgggcgcgg
      901 tcggtgtcga attgctcgcg cgccttggcc gagaacatgc acggcaccga gacggcggtt
      961 tcagtgccgc aggaccaggc gttggggtag ttgacgatgt cggtgcgggc ggccagctcg
     1021 ggcgtggtgg ggcgcgggta gccgttgagg gcaaagtggt cagcgcgggc ggtttcgccc
     1081 accaccagca ccagcacgcg cggtttggcg tgcgcggcgg cggtttgttt ggcgtccagg
     1141 ccaatttgct gcaagggctg cggtttggcg gtgactttgc gcaaataaga ggtggacgct
     1201 tgcaggtaat tcagcggggc cagcgccatg cgcagttcgc ggtggttgcg aaacagcgag
     1261 gcaaagtcct gatagcccac cgcggcaatc gccgccagcg ccagggtggc gatgcccagg
     1321 tgcgccaagc gccgacccag ccgccgccag ccggggcggt attccaccgg cagtttccac
     1381 agcagccagc atggcagcac gcccagcagc agcacggtca gcgccatttt gacggtaagc
     1441 aggtcgcgca cttcggcgcc gtcggtttgc agggcgttgc gcaccatgtc ctggtcgatc
     1501 agcacgccgt actggttcat gaagtagctc accgctgcgg tggtcaacag caggaggcca
     1561 atcagcggct tgcccacccg tggccagaca aacagcagca acacccagta aaagaaggtc
     1621 gtggtcagcg ccagcgccag cgccagcatt ttcacgccat ggccatccag cgggctgaca
     1681 atttcccaca gccgttgcca gaacggcagg ttgaacaaca gcaccagcgc catggccaca
     1741 tacagggcca gacgctccgg cgacaacgga cgcagcaatt tcatgaggac acaacccgga
     1801 aaaaaatgag aagaattatc agcgcagcca gcttaatgcg ggcttaagaa ccgcacgcca
     1861 cacccggtac agccatccgg gcgacgcgcc aacatagaca aaccccgtcg ttcggcaagg
     1921 ccgaaaacaa ggtcagcagg acgtttgcgc gctgtgccac gcgccatcat acgcgccccg
     1981 acctcaatcc agatgacgct gtcgccagtc ttgcaacagc ggttcaatat catgcaggtt
     2041 gcgcaggatc tgccagtgtc cggccggcga aggacgcatg ccctgcacgc ccgcccctcc
     2101 cacccacagc tcgacctggg gcggcagcaa gcggcgcaag gcagtcaggg tatctggggg
     2161 cgcgccatcg taggcttgtg agaacgacaa cgccagcaca tccacccggt gcttgtgtgc
     2221 agcctgggca atgtcggtca gcggcaggtc ggcgccaaag gccagcacct cgcaggccga
     2281 caagcggaac aaggcctcgg ccatcagcaa gcccagcaca tgctgctcgc cggcacaggt
     2341 ggtgagcatc accttggggt ggccctgggg aatggtcagc accgccagcg tctcgcgcag
     2401 gatgcgggtg acctgctcgg tgtacaaatg ttcttcgtgg atttcgatct ggccgcgcgc
     2461 ccacagttca cccaccagct gattggccgg cggcagaaaa tcacagacaa agctgcgcag
     2521 cccctggctg tgcaactggc attgcagctc atcgcggatg gcgtggcctt cctggccttt
     2581 cagcaggctt tccagccggg cgcataccgc aggggcggct tcacgcgggc ggcgcgcctc
     2641 gaacaggcgg gataattctt cggcactcag atgaatgatc ttgcccggcc ggaatccctg
     2701 atccatcagc cggcgcacgg cgcgcagctt gtccacctgt tcgcgcgggt acacccgatc
     2761 cccctgggca tcgcgggccg gctcgggaaa gcc
//
LOCUS       DAOPYX010000356         2769 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_51131_length_2769_cov_8.520634, whole genome shotgun sequence.
ACCESSION   DAOPYX010000356 DAOPYX010000000
VERSION     DAOPYX010000356.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 2769)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2769)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2769
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51131_length_2769_cov_8.520634"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..779)
                     /locus_tag="PKN55_13295"
     CDS             complement(<1..779)
                     /locus_tag="PKN55_13295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012697532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNX72267.1"
                     /translation="MAETYQAFHRRSIEQPEAFWAEQAELIDWHKPFEQVLRFDNPPF
                     RDWFVGGETNLCHNAVDRHLKERADQNALIFVSTETDTEKTYTFAELHREVSRFAACL
                     QDLGVGKGDRVLIYMPMIPEAIFAMLATVRLGAIHSVVFGGFAAHSLATRVDDAQPKV
                     MVTADGGMRGGKIIPYKHLVDDALAQSEHPPASVLIVNRGLDPEMAIVAGRDVDYASL
                     RERHLDADVPCEWVESNHPSYILYTSGTTGKPKGVQRDTGG"
     gene            complement(1154..2080)
                     /locus_tag="PKN55_13300"
     CDS             complement(1154..2080)
                     /locus_tag="PKN55_13300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008952661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNX72268.1"
                     /translation="MSAASIRLPRLGGFSIGKGVVLAVLSAAGFSAKAIFVKLAYAYS
                     VDAVTLLLLRMVFALPFFMLMAGMGARGAAPMPRRAWALVLPLGFMGYYLSSVCDFIG
                     LQYVSSTLERLVLFLYPTFVLLFSALLFRRRVAGREWLALSLCYLGAGLAVTHDWQAL
                     GAGSNVLLGVSWVLASALSYSLYLIGVGELVKQAGALRLSAWLCMVATLAVMVHFALT
                     HPLSAALNLPWPVYAYGLAMALLSTVLPVVLMNEAMRQIGANQVALVSTLGPVMTLLL
                     GWLVLSESMSLLQLAGAALVVGGVASVSLRKK"
     gene            complement(2264..2623)
                     /locus_tag="PKN55_13305"
     CDS             complement(2264..2623)
                     /locus_tag="PKN55_13305"
                     /inference="COORDINATES: protein motif:HMM:NF039463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6463 family protein"
                     /protein_id="HNX72269.1"
                     /translation="MGLAMGGRRWRGRALCAIAGVHTLVAGVEFAPVWRELWAAGVVD
                     SVGRDPLRGAVAWFFLFGVLLALLGLAVDELERQTPAWHSPTLAVGLLVLVVIGVTLM
                     PASGFWLALPVAWRLWR"
BASE COUNT          638 a          990 c          742 g          399 t
ORIGIN      
        1 tagccgccgg tgtcgcgctg cacgcccttg ggcttgccgg tggtgccgga ggtgtacagg
       61 atatagctgg ggtgattgga ttccacccat tcgcacggca cgtcggcgtc cagatggcgc
      121 tcgcgcaggc tggcgtaatc cacatcacgg ccggcgacaa tcgccatctc cggatccaga
      181 ccacggttga cgatcagcac gctggccggc ggatgctcgg actgcgccag cgcgtcgtcc
      241 accagatgct tgtaaggaat gatcttgccg ccgcgcatgc cgccgtcagc ggtaaccatc
      301 accttgggct gggcgtcgtc cacgcgggtg gccaggctgt gcgcggcaaa gccgccaaac
      361 accaccgagt gaatcgcccc caggcgcacg gtggccagca tggcgaaaat cgcttccggg
      421 atcatcggca tgtagatcaa cacgcggtcg cccttgccca cgcccaggtc ttgcagacag
      481 gcggcaaagc ggcttacctc gcggtgcagc tcggcgaagg tgtaggtttt ttccgtgtcg
      541 gtttcggtgg acacaaaaat cagcgcgttc tggtcggcgc gctctttcag gtggcggtcc
      601 acggcgttgt ggcacaggtt ggtttcaccg ccgacaaacc agtcgcggaa cggcgggttg
      661 tcaaaacgca gcacctgctc gaaaggcttg tgccagtcga tcagctcggc ctgttcggcc
      721 caaaaagctt ctggttgctc gatggaacgg cgatgaaacg cctgataggt ttcggccatg
      781 gggtctctcc tccagcacaa agacaacccc gtggcggcac attttgatgg tgttggcggc
      841 tcggcacggg gcgaatattc tcctgaccaa gcaattgctt ggtagggagc aaccctacac
      901 gctaacgccg ggctggtcaa atcttcatgc tgttgcactg cgccagccat cttgcgcgat
      961 gattgaatgc tgcgacgcaa taatttgcca acaagcggta tagattagac ccatgccgtt
     1021 ttaatcagcc cattttccgc agtgcaaaaa cgccgagcgg gtgtgacgga attaccgcat
     1081 caggcacggg gttttccatc ccgccggcct gggcccaagc ctgaaagcag ggcgggagac
     1141 accccaccct gtgttatttc ttgcgcaaac tcacactggc caccccaccc accaccagcg
     1201 ccgcaccagc cagctgcaac agcgacatgc tctcggacaa caccagccag cccagcagca
     1261 acgtcatcac cggccccagc gtggacacca aggccacctg attggcgcca atctggcgca
     1321 tcgcctcgtt catcaacacc accggcagca ccgtgctcag caaggccatc gccagcccgt
     1381 aggcgtacac cggccacggc agattcagcg ccgccgacag cgggtgagtg agggcaaaat
     1441 gcaccatcac cgccagcgtg gccaccatgc acagccaggc cgataagcgc agcgcgcccg
     1501 cctgcttgac caactcgccc acgccaatca gatacagcga ataactcagc gcgctggcca
     1561 gcacccagct gacccccagc aagacattgc tgccagcgcc cagcgcctgc cagtcatgcg
     1621 tcaccgccag gcccgcgccc agataacaca gcgacaaagc cagccactcg cgcccagcca
     1681 cccggcggcg gaacaacagc gccgaaaaca gcagcacaaa cgtggggtag agaaacaaca
     1741 ccagccgctc cagcgtggag gacacatact gcaagccgat aaaatcacac acgctggaca
     1801 agtaataccc cataaagccc agcggcaaca ccagcgccca cgcccgacgc ggcatcggcg
     1861 ccgccccacg cgcgcccatg ccggccatca gcatgaaaaa cggcagcgca aacaccatgc
     1921 gcaacaacaa taaagtaaca gcgtccaccg aataggcata cgccagcttg acgaaaatcg
     1981 ccttggcaga aaacccggcc gccgacaaca ccgccagcac cacgcctttg ccaatagaga
     2041 accctcccaa gcggggaagg cgaatcgacg ccgcagacat cacacgctcc tgagtcttga
     2101 tctgccggga agtatgcgca ggatacgccc gctttcccat cagtatttga cacactcagg
     2161 cttcgtgaaa tacgaaggcc atgcgaaact caagaccgac cttccacgat caatgttacg
     2221 gcagcgcacc gtaacgccgc ccgccaagca ggccaagccg cgctcaacgc cacagccgcc
     2281 acgccaccgg caaagccaac caaaagcccg acgccggcat cagcgtcacc ccaatcacca
     2341 ccaacaccag caaacccacc gccaacgtcg ggctatgcca agccggcgtt tgccgctcca
     2401 actcatccac cgccaggccc agcaaggcca gcagcacgcc aaacagaaaa aaccacgcca
     2461 ccgccccgcg tagcggatca cgcccgacgc tatccaccac cccggccgcc cacagctcgc
     2521 gccacaccgg ggcaaactcg acgcccgcca ccagggtgtg caccccggca atcgcacaca
     2581 atgcccgccc acgccaacga cgcccaccca ttgccaaacc catcgcccat cctcctgttg
     2641 cccatctccg gcatgccccg gtaagcgcga gaatacgccc gcaggtggcg cgggtcttgt
     2701 acgcagacga catcttgccg ccccggccga cggcgcttac actgccagac agacggctgg
     2761 ggaaagcat
//
LOCUS       DAOPYX010000357         2765 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_51252_length_2765_cov_4.183395, whole genome shotgun sequence.
ACCESSION   DAOPYX010000357 DAOPYX010000000
VERSION     DAOPYX010000357.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 2765)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2765)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2765
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51252_length_2765_cov_4.183395"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(130..1317)
                     /locus_tag="PKN55_13310"
     CDS             complement(130..1317)
                     /locus_tag="PKN55_13310"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNX72270.1"
                     /translation="MLLPFPAWADTDAPAANPYLEAMQPLIEGRFEEASEALHRLVAQ
                     EPEHAGAWLDLAILQCRLGNAEEAEILFQAIEVRFDPPPGIREIIALQRAKGCQAPAP
                     RWEGRLRLSRGSDDNVNQGASNPNFTIGQGSNLLSLVLSPEFTPQRDRFNNLSLEGSV
                     SGVNGWQGYAQLQARRYDHLSRYNSVSTSVGAERVRRFAGWDTRLGGSLGLTQLGGKD
                     YQRYAALNAWITPPLPLPAGWQLSGLTGVSHVVYPSRSYFDSSLWEARGVLTYQQGPV
                     WLQGSGGYGVDIGNGARAGGDRHGLFASLSARAALGGGVLGELGWLYQSWAGARSYSP
                     GLIDVARRQHTQLLRAMVSLPLSKEHGLFLEYRMVRNRENISLFAYQSRSVQLGWQWQ
                     FGR"
     gene            1557..>2765
                     /locus_tag="PKN55_13315"
     CDS             1557..>2765
                     /locus_tag="PKN55_13315"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HNX72271.1"
                     /translation="MGIHTPIRHLLAAVLFSLATVCWAGEAGKVVLSVGEVRLQGQPA
                     KAGNSVQPGDHLSTGADGYLYIKTVDNGFLILRPNSEASIVAYQADTDTPANSRFKIE
                     LQQGVARSISGQAVKNARQNFRFNTPIAAIGVRGTDFTVFTDAQTTRIAVLSGGVVVS
                     GFGQGCSPEGNGPCESPNSRELFARQSAEVLQISRGQQVPELIRGKALLPDVNTPPLP
                     DEPGKTSRNSIRRSTDTSLESSLVPLKLAELDKATVVNGSTAPTVTLPNSVAPISWGR
                     WKTIAEQSASINLDTLSAEGYRIAALTGDFALLRHQNASWQAPTGSLNFALQGSMAQF
                     QRNGATQLASLENASLSIDFDRSRFATQFDLVSQDIRLTRQAQGNVGSDGSFSSASQF
                     LEGNNMTVQGV"
BASE COUNT          577 a          895 c          804 g          489 t
ORIGIN      
        1 atcggcgtgt gaaaacagga acttgtaacc ggtgtcgtgc agatgtgcca tgggggtatt
       61 gtaggcatta ccgccggtga gaggcaaaca tcagggagtg ttgcaccggg atgatttccc
      121 tgtgttttgc taacggccaa actgccactg ccagcccagc tgcacgctac ggctctggta
      181 ggcaaacagg gaaatatttt cccggttgcg caccatgcga tactccagaa acagcccgtg
      241 ttccttgctc aacggcagcg aaaccatcgc ccgcaacaat tgggtgtgct ggcggcgggc
      301 cacgtcgatc aggccgggag aatagctgcg ggcaccggcc cagctctgat acaaccagcc
      361 cagttcaccc agcacgccgc cccccagcgc ggcgcgtgcc gacaggctgg caaacaggcc
      421 gtgccggtcg ccgccagcac gggcaccatt gccaatatcc accccatagc cgccgctgcc
      481 ctgtagccag acggggcctt gctgataggt cagcacgcca cgggcctccc acaggctgga
      541 gtcaaaatac gagcgcgacg ggtataccac atggctgacc ccggtcagcc cactcagctg
      601 ccagcctgct ggcagcggca gcggtggggt aatccaggca ttcagcgcgg catagcgctg
      661 gtaatccttg ccccccagct gggtcaaccc cagcgagccc cccagccggg tgtcccagcc
      721 agcaaagcgg cgcacccgtt ccgctcccac cgaggtggac acgctgttgt agcgcgagag
      781 atgatcgtaa cggcgggcct gcagctgggc atacccttgc cagccattca ccccactgac
      841 cgacccctcc agcgacagat tattaaagcg gtcgcgctgc ggcgtgaact cgggtgacag
      901 caccaggctc aacagattgc tgccctggcc gatagtaaag ttgggattgc tcgccccctg
      961 attgacattg tcgtcactgc cgcgactcag gcgcaggcgt ccttcccagc gcggtgcggg
     1021 tgcctggcat cctttggcgc gctgcagggc aatgatttcc cggataccgg gtggtgggtc
     1081 aaagcgcact tcaatggcct ggaacagaat ttcagcttct tcggcattgc ccagccggca
     1141 ttgcagaatc gccagatcca gccaggcccc ggcgtgttct ggctcctgcg ccaccagccg
     1201 gtgcaaagcc tcgctggcct cttcaaaacg gccttcgatc agcggttgca tcgcctccag
     1261 atacgggttg gcagcagggg cgtcagtgtc cgcccacgca gggaaaggca gcagcatcag
     1321 caggccgagc agccaggtgg cccagcaacg gatgccgcgt gtgtttgccg cttgtccggc
     1381 agaatgtggg accctcattc cagcaatttt tccgtgttgg ggaacaccca caatgctatg
     1441 ctatccatct acttgaatga tccatctacg ctatattcaa acatgcagcc aacccgctgg
     1501 cgaacacgcc gctggcatgg ctacatgaag agagtttgca cagggagtac cgaataatgg
     1561 gcatccatac ccctatccgc cacctgcttg ctgctgtgct gtttagcctg gccaccgtgt
     1621 gctgggccgg cgaagccggc aaggtggtgc tcagcgtggg cgaagtccgc ctgcagggcc
     1681 agccagccaa ggctggcaac agcgttcagc ctggcgacca cctgagcacc ggcgcagacg
     1741 gctatctcta tatcaagacc gtggacaacg gtttcctgat tttgcggccc aacagtgaag
     1801 ccagcattgt ggcctatcag gccgatactg acacacccgc caacagtcgg ttcaaaattg
     1861 agcttcaaca aggcgtggcg cgcagtattt ccggccaggc ggtgaaaaat gcccgacaga
     1921 atttccggtt caacacgcca attgctgcta tcggcgtgcg gggcaccgac tttaccgtat
     1981 ttaccgatgc acaaaccacc cgtattgcgg ttttatctgg tggcgtcgtg gtcagcggct
     2041 ttggccaggg ctgctcgcca gaaggcaatg gcccatgcga aagcccgaac agccgcgaac
     2101 tgtttgcccg gcaaagtgcc gaagtgctac aaatcagccg gggccagcaa gtgcccgaac
     2161 tgattcgagg caaggcgcta ttgcccgacg taaacacccc gccactgccg gacgagccgg
     2221 gcaaaacctc gcgcaacagc atccgccgct cgaccgacac cagcctggaa tccagcctgg
     2281 tgccgctcaa gctggccgag ctggataaag ccaccgtggt aaacggcagt acggcaccga
     2341 ctgtcaccct gcccaactcg gtggctccca tcagttgggg gcgctggaaa accattgccg
     2401 aacaatcggc cagtatcaat ctggataccc tgagcgccga aggctaccgg atagccgccc
     2461 tgaccggcga ctttgccctg ctgcgccacc agaacgccag ctggcaagcc cccaccggca
     2521 gcctgaactt tgccctgcag ggcagcatgg cgcagttcca gcgcaatggt gccacccagc
     2581 tggccagcct ggaaaacgcc agcctatcga tcgactttga ccgcagccgc tttgccaccc
     2641 agtttgacct ggtgtcgcaa gacatccgcc tgacgcgcca ggcgcagggc aatgttggca
     2701 gtgatggcag cttcagcagc gccagccagt ttctggaagg caataacatg accgtgcaag
     2761 gcgta
//
LOCUS       DAOPYX010000358         2736 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_52037_length_2736_cov_12.261470, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000358 DAOPYX010000000
VERSION     DAOPYX010000358.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 2736)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2736)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2736
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52037_length_2736_cov_12.261470"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            274..1920
                     /locus_tag="PKN55_13320"
     CDS             274..1920
                     /locus_tag="PKN55_13320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HNX72272.1"
                     /translation="MSQEAYRQGLDQNPANYVPLSPLTYLERAAYVYPTRLSVVYNDR
                     QFTWAQTYERCRRLASALVKAGVKPGDTVATMLPNVPAMYEAHFGIPMSGAVLNTLNT
                     RIDSESVAFMLRHGEAKVLLTDPEYADIVGNALEMMAPEERPRVVDVADATYAGPDYR
                     LGQQEYEDFIAGGDADFVWNLPGNEWDAIALNYTSGTTGNPKGVVYHHRGAYLNAVSN
                     IVSWGMQPHAVYLWTLPMFHCNGWCFPWTMAANAGINVCLRRVDVNRIFELIRTLKVT
                     HFCGAPIVHGMLINAPEAARTGIDHPVNALIAGAAPPAAIIEGMERIGFNITHVYGLT
                     ETYGPASVCAKHPEWDSLPIEQRAEMNGRQGVRYHMQEAVSVRDPITLEAVPADGETM
                     GEVMFRGNLVMKGYLKNETASAEAFAGGWFHTGDLAVMQPDGYIKIKDRSKDVIISGG
                     ENISSIEVEDVLYRHPAVSAAAVIAMPDPKWGEVPCAFLELKDGAETTEEAVIEYCRE
                     HLARFKVPKKVIFGPLPKTSTGKLQKYLLRQQMKSASAIG"
     gene            2000..>2736
                     /locus_tag="PKN55_13325"
     CDS             2000..>2736
                     /locus_tag="PKN55_13325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014087157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase N-terminal
                     domain-containing protein"
                     /protein_id="HNX72273.1"
                     /translation="MTSYTAPQRDMLFVLNHLAGLEAVSQLPGLEDATPDMAAAILDE
                     AAKFAAGVLAPINASGDLQGCRWDNGVVTPADGFADAYASFCETGWNAMPAQPEFGGQ
                     GLPSLVSSAVQEMWKSANMAFALCPMLTLGAVNAIAEHASDELKAVYLPKMVSGEWTG
                     TMNLTEPNAGSDLAAVRSRAVPAGDGSYRISGTKIFITWGEHDMAENIVHLVLARLPD
                     APEGVKGISLFIVPKYLVNADGSLGAR"
BASE COUNT          530 a          892 c          832 g          482 t
ORIGIN      
        1 ctaaccaaaa attcagcaac accagtcact tacccgctgc aaacattcgt tacatttgca
       61 caatagttcc gacaccttct cctgcgaatc tcccagtgtc acaagcgtcc cctgtgacgg
      121 tacacctgtt tcctccccgg ccgcactccc cgtcggccgg ggtgtacccg gtgtcataga
      181 ataagagccg tcaacaaaac gcagcacatg cgtacggcct ggctgcgcaa ttccgccccg
      241 gttttgttgg ccgacccaag gagaggagaa gtcgtgtctc aagaagccta tcgtcaaggt
      301 ctggaccaaa atccggccaa ctatgtcccg ctgtccccgc tgacctatct cgaacgcgcc
      361 gcttatgtgt accccacccg cctgtcggtg gtgtacaacg accgccagtt cacctgggcg
      421 caaacctacg agcgttgccg ccgtctggcc tcggcgctgg tgaaggcggg cgtcaagccc
      481 ggcgacacgg tggccaccat gctgcccaat gtgccggcga tgtacgaagc gcactttggc
      541 atcccgatgt ctggcgcggt gctgaacacc ctgaacaccc gcattgactc cgaatcggtg
      601 gccttcatgc tgcgccacgg cgaagccaag gtgctgctga ccgacccgga atacgccgat
      661 atcgtcggca atgcgctgga gatgatggcg ccggaagagc gtccgcgcgt ggtggacgtg
      721 gctgacgcca cctatgccgg cccggactat cgcctgggcc agcaagaata cgaagatttt
      781 attgccggcg gcgacgctga ttttgtctgg aatctgccgg gcaatgagtg ggacgccatc
      841 gcgctgaact acacctccgg caccaccggc aaccccaagg gtgtggtgta ccaccatcgc
      901 ggcgcctacc tgaacgcggt gagcaatatc gtgtcctggg gcatgcagcc gcatgcggtg
      961 tacctgtgga cgctgccgat gttccactgc aatggctggt gtttcccgtg gaccatggcc
     1021 gccaacgccg gcatcaatgt ctgcctgcgc cgcgtggacg tgaaccgcat ctttgaattg
     1081 atccgcacgc tgaaagtcac ccatttctgc ggtgcgccga ttgtgcacgg catgctgatc
     1141 aatgcgccgg aagccgcgcg caccggcatc gatcacccgg tgaacgccct gattgccggc
     1201 gcggcgccgc ccgctgccat tatcgaaggc atggagcgca tcggctttaa catcacccac
     1261 gtttacggcc tgaccgaaac ctatggcccg gcctcggtgt gcgccaagca cccggaatgg
     1321 gacagcctgc cgattgaaca gcgcgccgag atgaacggcc gccagggcgt gcgctaccac
     1381 atgcaagaag cggtgtcggt gcgcgacccg atcaccctcg aagccgtgcc ggccgatggc
     1441 gaaaccatgg gcgaggtgat gttccgcggc aatctggtga tgaagggcta tctgaaaaac
     1501 gaaaccgcca gcgccgaggc gtttgccggc ggctggttcc acactggcga cctggcggtg
     1561 atgcagccgg atggctacat caagatcaaa gaccgctcca aggacgtgat catctccggc
     1621 ggcgagaata tctcgtcgat tgaagtcgaa gacgtgctgt atcgccaccc ggcggtgtcg
     1681 gcggcggcgg tgatcgccat gcccgacccg aaatggggcg aagtgccgtg cgccttcctg
     1741 gagctgaaag atggcgcgga aaccaccgaa gaagcggtga ttgaatactg ccgcgagcat
     1801 ctggcccgct tcaaggtgcc caagaaagtg atcttcggcc cgctgccgaa aacctccacc
     1861 ggcaagttgc agaaatacct gctgcgccag cagatgaagt cggcctcggc catcggctaa
     1921 gccgtctgca ccctaacgcc tatctagcct gacacgccgc cccgcccacc ggggcggctt
     1981 ccgagagcac acacccatga tcacgagtta caccgcgccg cagcgcgaca tgctgtttgt
     2041 cctcaaccac ctggccggcc tggaagccgt ctcgcaattg ccgggtctgg aagacgccac
     2101 cccggacatg gccgcggcca ttctggacga agcggccaag tttgccgctg gcgtgctggc
     2161 gccgatcaac gccagcggcg accttcaggg ctgtcgctgg gacaacggcg tggtcacccc
     2221 ggccgacggc tttgccgacg cctacgccag cttctgtgaa accggctgga acgccatgcc
     2281 ggcgcagccg gagtttggcg gccagggctt gccctcgctg gtgtcgtccg ccgtgcaaga
     2341 gatgtggaag tcggccaata tggcctttgc cctgtgcccg atgctgacgc tgggcgcggt
     2401 gaacgccatt gccgaacacg ccagcgacga gctcaaggca gtgtatctgc ccaagatggt
     2461 gtccggcgag tggaccggca ccatgaacct gaccgaaccc aacgccggct ctgatctggc
     2521 cgccgtgcgc agccgcgccg tgccggctgg cgacggcagt tatcgcatca gcggcaccaa
     2581 gattttcatc acctggggtg agcacgacat ggccgagaac atcgtccatc tggtgctggc
     2641 ccgtctgccg gacgcgccgg aaggggtgaa gggcatttcg ctgtttatcg tgccgaaata
     2701 cctggtcaac gccgacggct cgctgggcgc gcgcaa
//
LOCUS       DAOPYX010000359         2728 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_52263_length_2728_cov_11.153760, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000359 DAOPYX010000000
VERSION     DAOPYX010000359.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 2728)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2728)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2728
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52263_length_2728_cov_11.153760"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..578)
                     /gene="metW"
                     /locus_tag="PKN55_13330"
     CDS             complement(<1..578)
                     /gene="metW"
                     /locus_tag="PKN55_13330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012698401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine biosynthesis protein MetW"
                     /protein_id="HNX72274.1"
                     /translation="MSLRSDLQKIANDIPPGAHVLDLGCGDGELLAYLAARGVSGYGV
                     EIDVDNVIACVERGVDVIQGDLEAGLAGFADQSFDVVVLSQTIQAMKQTEQILLDMLR
                     VGREAIVTFPNFGYWQNRLQILLHGRMPVSETIPYAWYDTPNLHLCMLGDFEALCAKN
                     GIRVVERVVMDGEQEITFLPNLLGSLALYRVSR"
     gene            complement(575..1717)
                     /locus_tag="PKN55_13335"
     CDS             complement(575..1717)
                     /locus_tag="PKN55_13335"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018748344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HNX72275.1"
                     /translation="MTPDPHSVGLVTPQTALFDTPLTLASGATLEHYQLVFETYGELN
                     ADASNAILICHALSGHHHVAGRHHPDDKAAGWWDNMIGPGKPIDTSRFFVVGLNNLGG
                     CHGSTGPSSINPATGQPWGASFPVVTVPDWVHAQARLADRLGIERWAAVIGGSLGGMQ
                     ALQWTLTYPDRVAHALVIASAPKLTAQNIAFNDVARQAILTDPDFHGGDYYAHGAVPR
                     RGLRLARMLGHITYLSDDGMGAKFGRMLKSGEYRYGYDVEFEIESYLRYQGDKFSDYF
                     DANTYLLMTKALDYFDPAQAFAGNLAAALGQAKAQFLVASFTTDWRFSPARSKEIVKA
                     LIDAKRRVSYAEIESTHGHDAFLMTDAPYVRLMRAYLNNVAKEVGA"
     gene            1906..2085
                     /locus_tag="PKN55_13340"
     CDS             1906..2085
                     /locus_tag="PKN55_13340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008952230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX72276.1"
                     /translation="MFGRLFKFFLLATVLVLLVRLLFSRQQRHGLREWITTLAQALLI
                     SSALFVAAYWLWGVH"
     gene            complement(2128..2691)
                     /locus_tag="PKN55_13345"
     CDS             complement(2128..2691)
                     /locus_tag="PKN55_13345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4337 domain-containing protein"
                     /protein_id="HNX72277.1"
                     /translation="MADEKKTEDKKDPWLNHLALTTVIFAVCATLSTFKGGGYSTRSV
                     ISQAQASDQWAYYQAKSIKANLYQIQRERLQLEFDTLPADAPASKRAAYQAKLDDYSA
                     KIKKYDAEKEDIQRTAHELEAKRDDAQRHGQPMGLAVIFLQIAILLSSVAGLFKRREL
                     WLCALPLGLLGLVLFANGFLLFLPPLL"
BASE COUNT          502 a          814 c          878 g          534 t
ORIGIN      
        1 cgactcaccc gatacagcgc caggctgccc agcagattgg gcaggaaggt gatttcctgc
       61 tcgccgtcca tcaccacccg ctcgaccacg cggatgccgt ttttggcgca cagtgcttca
      121 aagtcgccca gcatgcacag gtgcaggttg ggggtgtcgt accaggcgta ggggatggtt
      181 tccgacaccg gcatgcggcc gtgcagcagg atttgcaggc ggttttgcca gtagccgaag
      241 ttggggaagg tgacaatcgc ctcgcggccg acgcgcagca tgtccagcag gatttgctcg
      301 gtctgtttca tcgcctgaat ggtctgcgac agcaccacca cgtcaaacga ctggtcggca
      361 aagccggcca ggccggcttc caggtcgccc tggatcacgt ccacgccgcg ctcgacgcag
      421 gcaatcacat tgtccacgtc gatttccacg ccatagcccg acacgccgcg tgcggccaga
      481 taggccagaa gctcgccgtc gccgcagccc aggtccagca catgggcgcc ggggggaatg
      541 tcattggcga ttttttgcag gtcgctgcgc aggctcatgc gcccacctcc ttggctacat
      601 tgttcaggta ggcgcgcatc aggcgcacat acggggcgtc ggtcatcaaa aaggcgtcgt
      661 ggccgtgggt ggattcgatt tcggcgtaag acacccggcg cttggcgtcg atcagcgcct
      721 tgacgatttc tttcgagcgc gcgggcgaaa agcgccagtc ggtggtgaac gaggccacca
      781 gaaactgcgc cttggcctgg cccagcgcgg cggccagatt gccggcaaac gcctgagccg
      841 ggtcgaagta atccagcgcc ttggtcatca gcaaataggt gttggcgtca aaataatcag
      901 agaacttgtc gccctgatag cgcagatagg attcgatttc gaattccaca tcgtagccat
      961 agcggtattc gccgcttttc agcatgcggc caaatttggc gcccatgccg tcgtcgctca
     1021 gataggtgat gtggcccagc atgcgcgcca ggcgcaggcc acggcgcggc acggcgccat
     1081 gcgcgtaata atcgccgcca tgaaaatccg ggtcggtgag gatggcctgg cgggcgacgt
     1141 cgttaaaggc gatgttctgc gcggtgagct tgggcgccga ggcaatcacc agcgcgtgcg
     1201 ccacccgatc agggtaggtg agcgtccatt gcagcgcctg catgccgccc aggctgccgc
     1261 caatcaccgc cgcccagcgc tcgatgccca gccggtcggc cagccgcgcc tgcgcgtgca
     1321 cccagtccgg cacggtgacc accggaaaac tcgcccccca gggctggccg gtggccgggt
     1381 tgatgctgga tggcccggtg ctgccgtggc agccgcccag attgttcagg cccaccacaa
     1441 aaaagcgcga ggtgtcgatc ggcttgccgg ggccgatcat attgtcccac cagccggcgg
     1501 ctttatcgtc cgggtgatgg cggccggcga catgatggtg gccagacagc gcatgacaga
     1561 tcaggatggc gttgctggcg tcggcgttca gctcgccata ggtttcaaac accagttgat
     1621 agtgctcaag cgtggcgccg ctggccaggg tcagcggcgt gtcgaagagg gcggtctgcg
     1681 gcgtgacaag gccgacggaa tgcgggtctg gggtcatctg gcgtatcagg tctggttagg
     1741 ctcgcgggag acaagtcagt taaatagcac gtccggggcg atggctcaag caaatggaat
     1801 atttttgagc atggcatgcc gctggcgtgg cgtgtgcgcg gcgtcagctt gcccggcgca
     1861 gtcggcatgc ccataatcga cgccgctgtg ttgacagggt ggatcatgtt cggacgattg
     1921 ttcaaattct ttctgctggc caccgtcttg gtgttgctgg tgcgcttgct gttttcgcgc
     1981 caacagcgcc acggcctgcg cgagtggatc accacgctgg cgcaggcgct gctcatcagc
     2041 tcggcgctgt ttgtggccgc ctactggcta tggggcgtgc actgacgccg ccagcaggca
     2101 aacgaacact gtaaaccgcc gctgcggcta cagcaagggc ggcaggaaca gcaaaaagcc
     2161 gttggcgaac aacaccaggc caagcagccc cagcggcagc gcgcacagcc acagctcacg
     2221 gcgcttgaac aggccggcca ccgacgacag caagatggcg atttgcaaaa aaatcaccgc
     2281 cagccccatt ggctgaccat gacgctgggc gtcgtcacgc ttggcttcca gctcgtgggc
     2341 ggtgcgctgg atgtcttctt tttcggcgtc gtatttcttg atcttggcgc tgtagtcgtc
     2401 caatttagcc tgataggccg cgcgcttact tgctggggca tcagccggca gggtgtcgaa
     2461 ctccagttgc aggcgttcgc gctggatttg gtacagattg gccttgatgc tcttggcctg
     2521 atagtaggcc cactgatccg aggcctgcgc ctgactgatc acgctgcggg tggaataacc
     2581 gccgcccttg aaggtggaca gcgtggcgca tacggcaaag attaccgtgg tcagcgccag
     2641 atgattcagc caggggtctt tcttgtcttc ggtttttttt tcgtctgcca tggattatgt
     2701 caaatcaaag tcgggcacaa tccgatat
//
LOCUS       DAOPYX010000360         2727 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_52295_length_2727_cov_10.984656, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000360 DAOPYX010000000
VERSION     DAOPYX010000360.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 2727)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2727)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2727
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52295_length_2727_cov_10.984656"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1377
                     /locus_tag="PKN55_13350"
     CDS             <1..1377
                     /locus_tag="PKN55_13350"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNX72278.1"
                     /translation="YAGKEYYSLYDMHPRMVWHPIKPELNGKDLSTLKDPNGKLLVKE
                     MRDAVANQGSGFVDYLWPQPGKDEPVAKLAYAEGFAPWGWIVASGLYLDDLNAAFRHD
                     ALMFVGQIAVLSLLLGWLAFAIKWALTHPVHELMRTMAKVSEQRDLTCRAGIHSHDEL
                     GAVGRAFNQLLTHIQQTLSEVWGGSERLASRATSLSDHADRLAGASAEQNTAVSSSAA
                     ALEEISVSVEQVSAMAERLEQHADTARQHTQAGVSALGELCRHLDQADQQLSRDVSDS
                     ARHMLGSMGEISQMTGQVREIADQTNLLALNAAIEAARAGEAGRGFAVVADEVRKLAE
                     KSALSAGQIDTLTLRLQEASEAMQRAIADSQHALANSRSEAGVAAERMELASQTVSDT
                     RSSIHEMATALTEQSQSVQSIARDMQVIAGQSETQHAVVRDSAGAAGELEALAMELRG
                     AVGQFKTA"
     gene            complement(1453..>2727)
                     /locus_tag="PKN55_13355"
     CDS             complement(1453..>2727)
                     /locus_tag="PKN55_13355"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNX72279.1"
                     /translation="PNGNKLGELFASAIRSGGGKGGFAEYVWEKNKDVGVVGKVSYLG
                     VSSGWNWVIVTGVYLDDVEAAFRVQLTQLLVAGGVVLLIMWGASELIKRSVLRQLGGE
                     PAYAAEVVRTIASGDLTRQVNISGNPDSLLAAIAGMQRDLHQLIGRISGNADSLTQMA
                     QSLASHASEVTSVSDSQSSSASAMAASIEEMTASISQIADHAGAARDVSAESGQLSSD
                     GAKVIADAVDEMRRIHDAVSDTSMAITDLAGKTETISSIMNVIKEVADQTNLLALNAA
                     IEAARAGEQGRGFAVVADEVRKLAERTSSATEEIARMIADIKESSGQSQETMDEAMNR
                     VNHGLALAEQGGESIARIRDSAVRVVQVVNEISQALKEQSVASNDIALNVERIAQSAS
                     GNAQAARQVAQASSSMRDLTSELHHAVHRFSV"
BASE COUNT          517 a          884 c          842 g          484 t
ORIGIN      
        1 tacgccggca aagagtatta cagcctgtac gatatgcatc cgcgcatggt ctggcacccg
       61 atcaaacccg agctcaacgg caaggatttg tccacgctga aagaccccaa cggcaaattg
      121 ctggtgaaag aaatgcgcga cgccgtagcc aatcagggca gcggctttgt cgattatctg
      181 tggccgcagc cgggcaagga tgagccggtg gccaagctgg cctatgccga aggctttgcg
      241 ccctggggct ggattgtcgc ctctggcctg tatctggacg acctcaacgc tgcgttccgc
      301 cacgatgcgt tgatgtttgt cggccagatc gccgtgctct cgctgctgct gggctggctg
      361 gcatttgcca tcaaatgggc gctgacccac ccggtgcatg agctgatgcg caccatggcc
      421 aaggtcagcg aacagcgcga cctgacctgc cgcgccggca ttcacagcca cgacgaactg
      481 ggcgcagtgg gccgcgcgtt taaccagctg ctgacccaca tccaacaaac cttatccgag
      541 gtatggggcg gcagcgagcg gctggccagc cgggcgactt ctttgtctga ccatgccgac
      601 cggctggccg gcgcttcagc cgaacagaac acggcagtga gctcgtcggc cgccgcgctg
      661 gaagaaatca gcgtcagcgt cgagcaggtc agcgccatgg cggaacggct ggaacagcat
      721 gccgacaccg cgcgccagca tacccaggcc ggggtcagtg ccttgggtga gctgtgccgt
      781 catctggatc aggccgacca gcaactgagc cgcgacgtca gcgacagtgc ccgccatatg
      841 ctgggcagca tgggagaaat ttcacaaatg accggccagg tgcgggaaat cgccgaccag
      901 accaatctgc tggccttgaa cgccgcaatt gaagccgccc gcgctggcga agccggccgc
      961 ggctttgccg tggtggccga tgaggtgcgc aagctcgcgg aaaaatccgc gctgtcggcc
     1021 gggcagatcg acacactgac cttgcgcctg caagaagcca gcgaggccat gcagcgcgcc
     1081 atcgccgaca gtcagcatgc cctggccaat agccgcagcg aagccggggt ggccgccgag
     1141 cgcatggaac tggccagcca gacagtcagc gacacccgtt ccagcattca cgaaatggcc
     1201 accgcactga ccgaacaaag ccagtcggtg cagtcgatcg cccgtgatat gcaggtgatt
     1261 gccggccaga gcgaaaccca gcacgccgtg gtgcgcgaca gcgctggcgc cgctggcgag
     1321 ctggaggcgt tggccatgga actgcgcggg gcggtggggc aattcaaaac cgcctgaacg
     1381 acaccgatag aaaaaaatca cgcccccgtg cttcgatagc acgggggcgt ggttttgtgt
     1441 gacgcgagtg aattacaccg agaagcggtg tacggcgtgg tgcagctcgc tggtcagatc
     1501 gcgcatcgac gagctggcct gcgccacctg ccgcgcagcc tgggcattgc ccgaggcgct
     1561 ttgcgcaatc cgctcgacat tcagcgcgat gtcgttgctg gccacgcttt gttctttcag
     1621 cgcctgcgag atttcattga ccacctgcac cacgcgcacg gcgctgtcgc ggatgcgggc
     1681 gatggattcg ccgccctgct cggccagcgc caggccatgg ttcacccggt tcatcgcctc
     1741 gtccatggtt tcttgcgatt ggccagagct ttccttgatg tcggcaatca tgcgggcgat
     1801 ttcttcggtg gcggacgagg tgcgctcagc cagcttgcgc acttcatcgg ccaccacggc
     1861 aaagccacgg ccttgctcgc cggcgcgggc ggcctcaatc gccgcgttca gcgccagcag
     1921 gttggtctgg tcggccactt ccttgatgac gttcatgatg ctggaaatgg tttcggtttt
     1981 gccggccaga tcggtgatgg ccatcgaggt gtcgctgacg gcgtcgtgga tgcggcgcat
     2041 ttcatctact gcgtcggcaa tcaccttggc gccgtcactg gacagctggc cagattcggc
     2101 cgacacatcg cgcgccgcgc cagcatggtc ggcaatctgc gagatgctgg cggtcatttc
     2161 ttcaatcgaa gcggccatgg ccgaggccga gctgctttgc gagtccgaca cggaagtcac
     2221 ttcgctggcg tggctggcca gcgattgcgc catttgcgtc aacgaatcgg cattgccgct
     2281 gatacggcca atcagctggt gcaagtcgcg ctgcatgccg gcaatcgccg ccagcagact
     2341 gtcggggttg ccgctgatat tcacctggcg ggtcaggtcg ccactggcga tggtgcgcac
     2401 cacttcggca gcataggccg gctcgccacc caactgacgc agcacgctgc gcttgatcag
     2461 ctcactggcg ccccacatga tcagcagcac caccccgccg gccaccagca attgcgtcag
     2521 ctgcacacga aacgccgctt caacgtcatc cagatacacc ccggtgacaa tcacccagtt
     2581 ccagccagac gacaccccca ggtaagacac cttgcccacc acgccaacgt ctttgttttt
     2641 ctcccacaca tactcggcaa aaccgccctt gccgccgccc gaacggatgg cgctggcaaa
     2701 cagctcgccc agcttgttgc cattggg
//
LOCUS       DAOPYX010000361         2722 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_52448_length_2722_cov_6.156355, whole genome shotgun sequence.
ACCESSION   DAOPYX010000361 DAOPYX010000000
VERSION     DAOPYX010000361.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 2722)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2722)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2722
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52448_length_2722_cov_6.156355"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..227)
                     /locus_tag="PKN55_13360"
     CDS             complement(<1..227)
                     /locus_tag="PKN55_13360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX72280.1"
                     /translation="MIDDYPAPNTRPHLSKWLLIATDLAMLLLANRLGFWLAERVRIW
                     RDLPAFVASADSGLRLTVWLMLLLAAISWFWL"
     gene            492..2057
                     /locus_tag="PKN55_13365"
     CDS             492..2057
                     /locus_tag="PKN55_13365"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HNX72281.1"
                     /translation="MEFNVKDYGALGDGVSNDTQAFIAASAALSAHGGGVLRIPQGVY
                     VVGEQTLAGQLGLGYAYRSTDIISIVNCQQPVVIEGNHAVLRAAPGLRYGSFDPVSGE
                     PYAPAVMPFIDPDYAAGAYRMLVLANNRHVLVRDLELDGASDTLILGGGWGDLGRQLN
                     AYGVYALNNNTLQVENVHTHHHGLDGILVSQVGTTAQSPRTPATLIRVRSEYNGRQGL
                     SWTGGNGLTAIDCQFNHTGRGALSSSPAAGIDIEAEDGVVRNGLFINCEVINNVGPGM
                     GADSGDSADITFQRCTFIGSTNYPLWPRKPRLHFLDCTIAGQLVNVYAEQTPGDGNAT
                     RFTGCRFTDQLNWNGQVHLYPGWYLLDLGGGSQGVMFDRCSMDTQHGPLIYTAGEITL
                     RDCRLTQTTSHESTFSACYVGYNLAFSPPGAFTTGMARVLGQLLVNGEEAPQYVGIQR
                     KQCLQGNNGGAYAVRQSIAYAYSPEAAVGAGQANRGDIVYNPYAAPGGYAGWICVEAG
                     SPGIWKPFGPIAL"
     gene            complement(2059..2229)
                     /locus_tag="PKN55_13370"
     CDS             complement(2059..2229)
                     /locus_tag="PKN55_13370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX72282.1"
                     /translation="MASSFQDGNKIESVASRQAQKNQQITMDRRSSFHREISNPMLSN
                     VSIKPVQQKNSG"
     gene            2228..2698
                     /locus_tag="PKN55_13375"
     CDS             2228..2698
                     /locus_tag="PKN55_13375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX72283.1"
                     /translation="MTTPIPITIDVTAAPYSADNTGVSNATQAFIDASAALCAAGGGT
                     LLIPPGTYTVGRQVRATQSNQGYAYLGEDIITLSGCSHPVVIEGTGATLTLANGLKFG
                     SFDWSTGTAYTPASLPFTDANYAASVGRMLVVKDNPGHVVVRGLELRAANKTYR"
BASE COUNT          525 a          759 c          843 g          595 t
ORIGIN      
        1 agccagaacc agctgatggc agccagcagc agcatcagcc agacggtcag ccgcagacca
       61 ctgtcggcac tggccacaaa ggccggcagg tcacgccaga tgcgcacacg ttcggccagc
      121 cagaagccca gccggttggc cagcagcagc atggccagat ctgtggcaat caacagccat
      181 ttgctcaggt ggggacgggt attcggggca gggtaatcgt caatcatttt ttcttatcag
      241 tgagtggcat ggcgcggtgg gcccgcgcgt ggcggcgatt ttccttgagc cgggcaacgg
      301 cgacaagcga tgtgcagcaa atttgtgtga agaatgcttg acagtgcccc cgtcagtggc
      361 tgaccccggt caactgcgcg gcggcggtgt cgggctgcat gctgtgctgt cccgccatgg
      421 ctccggcatg ccatgtatct gatcagcggt gagcggggct tgctctgtat gccggctttt
      481 ggagattgca catggaattc aatgtcaagg attacggcgc gttgggcgat ggtgtcagta
      541 atgataccca ggcgtttatc gccgccagtg cggcgctgag tgcccacggt gggggtgtgt
      601 tgcgcattcc gcaaggggtg tatgtggtgg gcgagcaaac actggctggg cagcttggcc
      661 tgggctatgc ctatcgcagc acggacatca tttcgattgt caattgtcag cagccagtgg
      721 tgattgaagg caaccatgcc gtgctgcgtg cggcaccagg cctgcgctat ggcagtttcg
      781 acccggtgtc gggcgagccc tacgccccgg ccgtcatgcc gtttatcgat ccggattacg
      841 ctgccggcgc ttatcgcatg ctggtgctgg ccaataaccg ccatgtgctg gtgcgcgatc
      901 tggaactgga tggcgcatcg gatacgctga ttctgggcgg tggttggggg gatctcggcc
      961 gtcagcttaa cgcctatggg gtgtatgcac tgaataacaa caccttgcag gtggaaaacg
     1021 tccataccca ccaccatggc ctggacggca tcctggtgtc gcaggtgggc accacggcac
     1081 agtcaccgcg tacaccggcc acgctgattc gtgtgcgcag cgaatacaac ggtcgtcagg
     1141 gcttgagctg gaccggtggc aatggtttga cggcgattga ctgccagttc aatcacactg
     1201 gccggggggc gctgtcgtcc tcgcccgctg caggcatcga catcgaggcc gaggatggcg
     1261 tggtgcgcaa tgggctgttc atcaattgcg aagtcatcaa taacgttggc cccggcatgg
     1321 gggcggatag cggtgacagc gcggatatta cctttcagcg ttgcaccttt attggctcca
     1381 ctaactaccc gctgtggccg cgcaaaccgc gtctgcattt tctggattgc accattgccg
     1441 gtcaactggt gaatgtctac gccgagcaaa cgcctggcga tggcaatgcc acccgcttta
     1501 ccggctgccg ctttaccgat cagctcaact ggaatggcca ggttcatctg tatcccggct
     1561 ggtatttgct ggatctgggc ggtggcagtc agggggtgat gtttgatcgt tgcagcatgg
     1621 atacccagca cggcccgctg atctacaccg ctggcgaaat caccctgcgc gactgccggt
     1681 tgacgcagac caccagccac gagagcacct tcagtgcgtg ttatgtgggc tacaacctgg
     1741 ccttcagtcc gcctggtgcg ttcaccacgg gcatggcgcg tgtgctgggg caattgctgg
     1801 tcaacggcga agaagcgccg caatacgtag gcatccagcg caagcagtgt ctgcaaggta
     1861 ataacggcgg tgcatatgcc gtccgtcaga gcattgccta tgcctactct ccagaagccg
     1921 ctgtgggcgc cggccaggcc aatcgtggcg acattgtcta caacccctac gcggcgccag
     1981 gtggctatgc cggctggatt tgcgtagaag ccggcagccc tggcatctgg aagccgtttg
     2041 gcccgattgc cctgtgattt agcctgagtt tttttgctgc accggcttga tgctgacatt
     2101 gctcagcatc gggttagaaa tctctctgtg gaatgatgat ctgcgatcca tggtgatttg
     2161 ttgatttttc tgggcttgcc tggaggcaac gctctctatt ttgtttccat cctgaaagga
     2221 ggatgccatg accactccca ttcccatcac gattgatgtg acggcagcac catattcggc
     2281 agacaacaca ggcgtcagca atgcgactca ggctttcatt gatgccagtg ccgcgctgtg
     2341 tgctgccggg ggcggcacct tgttgattcc gcctggcact tacacggtgg gccgacaagt
     2401 ccgtgccacc caaagcaatc agggttatgc ctatctgggt gaggacatca ttaccctgag
     2461 cggttgcagc catcctgtgg tgatcgaagg caccggtgcg accctgaccc tggcaaacgg
     2521 tctgaaattt ggcagttttg attggtctac cggaacggcc tataccccgg ccagcctgcc
     2581 gtttaccgat gcaaactatg ccgccagtgt cgggcgcatg ctggtggtga aagacaatcc
     2641 gggccatgtg gtggtgcgag gcctggagct tagagccgct aacaaaacct atcgataaat
     2701 cgtaaacaaa tcaatgaaca ag
//
LOCUS       DAOPYX010000362         2636 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_55109_length_2636_cov_5.972104, whole genome shotgun sequence.
ACCESSION   DAOPYX010000362 DAOPYX010000000
VERSION     DAOPYX010000362.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 2636)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2636)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2636
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55109_length_2636_cov_5.972104"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            382..1008
                     /locus_tag="PKN55_13380"
     CDS             382..1008
                     /locus_tag="PKN55_13380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011136189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1 family protein"
                     /protein_id="HNX72284.1"
                     /translation="MRNTYWLLALSLLPTLVGALIGVQMNFGFMRASPIVSSLLMMVV
                     LYGMMFAIEKNRYSSLGVGLMLGFTFLMGLLLGPLLQVALSLRNGGQLIAMAAGTTSL
                     AFFVLAGIASTTKHDLSFLSKFITVGAVVLLAAMVANIFLKLPALYLALNVGFILFSS
                     AVILYQIKEVVDGGEDSYISATLTLYVSLYNIFSSLLQLLMAFGGDRD"
     assembly_gap    1086..1185
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1186..1641
                     /locus_tag="PKN55_13385"
     CDS             1186..1641
                     /locus_tag="PKN55_13385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008954113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HNX72285.1"
                     /translation="MTPITFASLPLSAASLLLDTAIAHAESLQLRVSVAVVDHAGHLL
                     GFKRMPGAALHTINIAQDKAYSAVSFGRPTGDWETRLAGQSDMLRHGLMLRERFVGFA
                     GGLPVLLDDARLGAIGVSGGSETQDLACAEAALAVLATAWRRNAAALPG"
     gene            2002..2601
                     /gene="rpoE"
                     /locus_tag="PKN55_13390"
     CDS             2002..2601
                     /gene="rpoE"
                     /locus_tag="PKN55_13390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HNX72286.1"
                     /translation="MSERDIDQQLVSRAQRGDKRAFDFLVNKYQRRLARLLSRFIRDP
                     AEIEDVTQEAFIKAYRALPSFRGDSAFYTWLYRIGINTAKNYLSASGKRPLLSGDYED
                     EDGDTVDLSAQVPDLNTPETEYMNRQIVETVNRAVDALPEELRMAITLREMEGMSYED
                     IAQAMDCPIGTVRSRIFRAREAISAQLKPLLDTAKNKRW"
BASE COUNT          489 a          784 c          741 g          522 t
ORIGIN      
        1 acctttcggc gctggaacct aaatccagtg cgtctaccaa ttccgccacc ctcgctaaag
       61 gaagaccgta tacttcggcc cgaacgagcc ggaaacacac ggttagccgt caaggataca
      121 tgaaccggct ttttgctgac aagccagctg tgacaaggcc ggaacttgtc gcccgcgcca
      181 cggtctgttg aacaccgcag ccaacgcggg atcgtcagca tgccaattgc ttgacgcatc
      241 gcgggttttt caggctacac tgcaagacaa gcgcattgcg cgcccttgct tacacacaaa
      301 tacacggaga atgtcaccat gtacacccag ttgtcttcag cctctgccag ttcgacactg
      361 gctgcaagcc gcaacaaggt catgcgcaat acctactggc tcttggcgct cagtctgctg
      421 cccacgctgg tcggggcgct gatcggcgtg cagatgaatt tcggcttcat gcgcgccagc
      481 ccgattgtct ccagcctgct gatgatggtg gtgctgtacg gcatgatgtt cgccatcgag
      541 aaaaaccgct acagcagcct gggtgtcggc ctgatgctgg gcttcacctt cctgatgggc
      601 ctgttgcttg gccccttgct gcaagtggcg ctgtcgttgc gcaatggcgg tcaactgatt
      661 gccatggcgg caggcaccac ctcgctggcc ttttttgtgc tggccggcat tgccagcacc
      721 accaagcatg atctgagctt tttgtccaag ttcattaccg ttggcgctgt ggtgctactg
      781 gcggcgatgg ttgccaacat cttcctgaag ctgccggcgc tgtatctggc cttgaacgtg
      841 ggtttcatcc tgttctcgtc agcggtgatc ctgtatcaga tcaaggaagt ggtggacggc
      901 ggtgaagaca gctacatctc ggccacgctg accctgtatg tcagtttgta caatatcttc
      961 tccagcctgc tgcaactgct gatggccttt ggcggcgacc gcgactgatc ccactcgctg
     1021 attcatcgca ccaagccctg aacacgcccg ccccgctttg ccggggcggg cgtgttttcg
     1081 tcgtgnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnatgac ccccatcacc
     1201 tttgcttcct tgcccttatc cgctgccagc ctgctgctgg acaccgccat cgcccacgcc
     1261 gagtcgctgc aactgcgtgt ttctgttgcc gtggtggatc acgccggcca cctgttgggt
     1321 ttcaagcgca tgcctggcgc cgcgctgcac accatcaaca tcgcccagga caaagcctat
     1381 agcgccgtca gctttggccg tcccaccggc gattgggaga cacggctggc cgggcaatcc
     1441 gacatgctgc gccacgggct gatgctgcgc gagcgctttg tcggctttgc cggcggcctg
     1501 ccagtgctgc tggatgacgc ccggctcgga gcgataggcg tgtctggcgg cagcgagaca
     1561 caagaccttg cctgtgccga ggccgcactg gccgtgctgg ccacggcctg gcgccgcaac
     1621 gctgctgcat tgcctggctg aatccagtac catgccggct ggcatcgcca ggcttaattg
     1681 gcgctgtacc ggaacaggtc tgcgctggat caacttttcc gcagaccctg gcgaacttgc
     1741 gtgcactcag taagtctact gagcagatca tccacctatc tggcaggatg gcaggcgccg
     1801 cccgcccgtg gcaacacgac gaggacggcc gccaaagctg gcttttgccg acgcgcgcgc
     1861 catcggtgcg cgacggtctg ctgctggcgt tgcgcgcagt caactgaata ataaaatcaa
     1921 gcagatgcgc ccatgcgcat ctggcaagat ctggggccat gccaaggcat ggctggcgcc
     1981 acgacgggag ccacttatcg catgagcgaa cgggatattg accagcaatt ggtcagccgg
     2041 gcgcagcggg gagacaagcg ggcttttgat tttctggtaa acaaatacca gcgccggctg
     2101 gcgcgtctgt tgtcgcggtt catccgcgac ccggcggaaa tcgaagatgt cacccaggaa
     2161 gccttcatca aggcgtatcg cgccttgcca agctttcgcg gagacagcgc gttttatacc
     2221 tggctgtacc gtatcggcat caatacggca aaaaactatc tctcggcatc gggcaagcgc
     2281 ccgctgctga gtggggatta cgaggatgaa gacggcgaca cggtggatct ctccgcccag
     2341 gtgcccgatc tgaacacccc ggaaaccgaa tacatgaacc ggcaaatcgt cgagacggtc
     2401 aatcgcgccg tcgacgcgct accggaagag ctgcgcatgg cgatcaccct gcgcgaaatg
     2461 gaaggcatga gctacgagga catcgcccaa gccatggatt gcccgatcgg caccgtgcgc
     2521 tcgcggattt tccgcgcccg cgaggcgatt tccgcccagc tcaaaccgtt gttggacacg
     2581 gccaagaaca aaaggtggtg aagcatgaac gaacaattct ctgcgctggt ggatgg
//
LOCUS       DAOPYX010000363         2626 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_55431_length_2626_cov_8.187865, whole genome shotgun sequence.
ACCESSION   DAOPYX010000363 DAOPYX010000000
VERSION     DAOPYX010000363.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 2626)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2626)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2626
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55431_length_2626_cov_8.187865"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(199..912)
                     /locus_tag="PKN55_13395"
     CDS             complement(199..912)
                     /locus_tag="PKN55_13395"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HNX72287.1"
                     /translation="MSAQKPDLPTADSDGGRDEAMYRGLHAAIYELRLPPGTRLPEDV
                     LSNTYGVSRTLVRKVLQRMAMEGLVEIRRNRGAQVAEPSADEAREVFATRRMLECGAL
                     AELPLPVPTAELQALRELVRQEDDAQLNGDRVAAIRLSGDFHIRLAALGGNATLTKLV
                     RELVARSSLILATHGSPISVTCRHSEHEQIIDLLARGERQAGVAWMAEHFEHITRGLR
                     WREQPLDLFSVLKGGDTPT"
     gene            1226..>2626
                     /locus_tag="PKN55_13400"
     CDS             1226..>2626
                     /locus_tag="PKN55_13400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007114988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS1 family nucleobase:cation symporter-1"
                     /protein_id="HNX72288.1"
                     /translation="MPSVLTRAPSERLHNEDLAPLQEQNWTWYNIFAFWMSDVHSVGG
                     YVFAGSLFALGLSSWQVLVSLIVGICIVNVMCNWVAKPSQQAGVPYPVACRMAFGVFG
                     ANVPAIIRGLIAVAWYGIQTFLASSALNILLLRFFPGLGELAHSHLLGLSYLGWMGFM
                     IMWLLQAVVFWHGMESIKKFIDWAGPAVYAVMFLLAGWIVWKAGPANISLNLGEVKYS
                     GWAAAGMMLTAVALVVSYFSGPMLNFGDFSRYGRSFAEVKRGNFWGLPVNFLAFSVVT
                     VVTTSGTLPVFGQMITDPIDTVSRIDNTLAAVLGAFTFVTATIGINIVANFVSPAFDF
                     SNVAPKHISFRAGGFIAAVASIFITPWNLFNSPEVIHYTLDMLAAFIGPLFGILLVDF
                     YLVRKQHIVVEDLYSEHPEGAYWYQGGVNRRALYALIPAVLLGIVITLSGAKGVADFN
                     WFIGCGVGALCYYALMK"
BASE COUNT          415 a          700 c          871 g          640 t
ORIGIN      
        1 cggtggtgtg cacagggcgg tgtggtgagc gggcatggtg gtcggtattg ggatggtttg
       61 tcttacatgc aagataataa tcaggttttt caggtttgga tggattcaaa acggctaatt
      121 caactgcctt gaaaaattga ttgtcataaa gtgtggccat gcccaacccc atccacccct
      181 atctgatttc agcccgcctc aggtcggggt gtcgccgccc ttgagcacgg agaacaggtc
      241 gagcggttgt tcgcgccagc gcaggccacg ggtgatgtgt tcgaagtgtt cggccatcca
      301 tgccacgccg gcctggcgtt cgccgcgcgc cagcaggtcg atgatttgtt cgtgttcgga
      361 gtggcggcag gtgacggaga tgggcgagcc gtgggtggcg aggatcagcg agctgcgcgc
      421 caccagttcg cgcaccagtt tggtgagcgt ggcgttgccg cccagggcgg ccaggcggat
      481 gtggaagtcg ccggagaggc ggatggcggc gacgcggtcg ccgttgagtt gggcgtcgtc
      541 ttcctggcgc accagttcgc gcagcgcttg taattcagcg gtgggcacgg gcagcggcag
      601 ttcggccagg gcgccgcatt ccagcatgcg gcgggtggcg aagacttcgc gggcttcgtc
      661 ggcgctgggt tcggccactt gtgcgccgcg attgcggcgg atttccacca ggccttccat
      721 ggccatgcgt tgcagcactt tgcgcaccag ggtgcggctg acgccatagg tgttggagag
      781 cacgtcttcc ggcaggcggg tgcctggcgg caggcgcagt tcgtaaatgg cggcgtgcag
      841 gccgcgatac atggcttcgt cgcgtccgcc gtcgctgtcg gcggtgggga ggtcgggttt
      901 ctgtgcgctc atggggtggg ccatccggct ggtttacagt gcgtgcactg cgagatgtgc
      961 actatggtgg cacattgttg cacacatgaa aagtatgttt tgtatacaaa attgcaatgc
     1021 tgtgtattcg atttaaagca tggcttgcgt ggccagacaa gggcttagcc acgaaaacgc
     1081 cttatttcac caggagataa actggatcag gcacatggat tgcttaggtt tgcccgcctg
     1141 aatgccatat ggcggcgcgc gctgggtggt aaattgtata caaactggcg cgccgacttg
     1201 tgttttgaac cccaaggagc cttccatgcc ctctgttttg acccgcgctc ccagcgagcg
     1261 gctgcacaat gaagaccttg ctccgctgca agagcaaaac tggacttggt acaacatctt
     1321 tgccttctgg atgtccgatg tgcacagcgt gggcggctat gtatttgccg gcagcctgtt
     1381 tgccctgggc ttgtccagct ggcaggtgct ggtgtcgctg attgttggca tctgcattgt
     1441 caatgtgatg tgcaactggg tggccaagcc cagccagcag gctggcgtgc cctacccggt
     1501 ggcgtgccgc atggcgtttg gcgtgtttgg cgccaatgtg ccggccatta ttcgcggcct
     1561 gattgccgtg gcctggtatg gcatccagac ctttttggcg tcctcggcgc tgaatatctt
     1621 gctgctgcgg ttttttcctg gtttgggcga gctggcgcac agccatttgc ttgggttgtc
     1681 ttacctgggc tggatgggtt tcatgatcat gtggctgttg caggcggtgg tgttctggca
     1741 tggcatggag tcgatcaaga agttcatcga ctgggcgggc ccggcggtgt acgcggtgat
     1801 gtttttgctg gccggctgga ttgtctggaa agccggcccg gccaatatca gcctgaatct
     1861 gggcgaggtg aagtacagcg gctgggctgc cgccggcatg atgctcaccg ccgtggcgct
     1921 ggtggtgtcg tatttttccg ggccgatgct gaattttggc gatttttctc gctacggccg
     1981 cagctttgcc gaggtcaagc gcggcaattt ctggggcttg ccggtgaatt ttctggcgtt
     2041 ttcggtggtg actgtggtga cgacgtccgg cacgctgccg gtgtttggcc agatgatcac
     2101 cgacccgatc gacacggtca gccgcatcga caacaccctg gccgccgtgc tgggcgcgtt
     2161 tacctttgtc accgccacca tcggcatcaa tatcgtcgcc aattttgtct cgccggcatt
     2221 tgatttttcc aatgttgcgc ccaagcacat cagctttcgc gccgggggct ttattgctgc
     2281 ggtggcgtcg atcttcatca ccccgtggaa tctgttcaac tcgcccgagg tgattcacta
     2341 caccctggac atgctggccg cctttatcgg cccgctgttt ggcatcctgc tggtggattt
     2401 ttatctggtg cgcaagcagc acatcgtggt ggaagacctg tatagcgaac accccgaagg
     2461 cgcttactgg tatcagggtg gggtgaaccg ccgcgcgctg tatgcgctga ttcccgccgt
     2521 gctgctgggc attgtcatca ccctgtccgg cgccaagggc gtggcggatt tcaactggtt
     2581 tatcggctgc ggcgtcggcg cgctgtgcta ctacgcgctg atgaaa
//
LOCUS       DAOPYX010000364         2608 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_56011_length_2608_cov_7.672542, whole genome shotgun sequence.
ACCESSION   DAOPYX010000364 DAOPYX010000000
VERSION     DAOPYX010000364.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 2608)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2608)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2608
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56011_length_2608_cov_7.672542"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(168..719)
                     /locus_tag="PKN55_13405"
     CDS             complement(168..719)
                     /locus_tag="PKN55_13405"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018748245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HNX72289.1"
                     /translation="MTHRLSTITTRTGDDGSTGLGDGSRVAKDCPRVRLLGEVDELNS
                     HLGVLLCEPLPDAARAALVDTQHDLFDLGGEIAVPGYQAIGEIQVLRLESWLATLNAD
                     LPPLREFILPGGCRAAALAHVGRAVARRAERQAVTLAREDIVSPHVLHYLNRLSDVLF
                     VLARHLNVAAGAADVLWRHPGGR"
     gene            1087..1494
                     /gene="msrB"
                     /locus_tag="PKN55_13410"
     CDS             1087..1494
                     /gene="msrB"
                     /locus_tag="PKN55_13410"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011136759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HNX72290.1"
                     /translation="MTDDLSRLSEAQWRARLTPEQFRVTRQGGTEYPFSGEHYRRSEP
                     GQYHCVCCGAHLFDADVKFDAGCGWPSFWQEAPGAGIRRLRDRSHGMIRTEVRCGQCD
                     AHLGHVFDDGPPPTGERYCINSVALTFTAQSAA"
     gene            1491..1922
                     /locus_tag="PKN55_13415"
     CDS             1491..1922
                     /locus_tag="PKN55_13415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009119145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HNX72291.1"
                     /translation="MNPPPLPALHHPRPWLRALFAIAGVLALLAGIIGIFLPVLPTTP
                     FVLLAAACFARSSPRIYGWLLRHRLFGPMIDTWQRERAVPRRVKWIALASMACSIGLS
                     LLLMQGQPWLQLLLAVIGLTLSAWLIRLPEPQLARANHAQR"
     gene            complement(2103..2417)
                     /locus_tag="PKN55_13420"
     CDS             complement(2103..2417)
                     /locus_tag="PKN55_13420"
                     /inference="COORDINATES: protein motif:HMM:NF016836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HNX72292.1"
                     /translation="MRRTLPASFALAALLALSVTGCAVSRDQSTVGQYVDDTGITARV
                     KARFAEDATVSAVAIRVETLRGVVQLSGFARSAAERVRAEALARATPGVAGVRNDIVV
                     QP"
BASE COUNT          529 a          931 c          744 g          404 t
ORIGIN      
        1 aaccttcaag gcgatgatcc gcgcccaagg tttgtttgcc ggcgaggcgg gggaagacag
       61 cgacgaagaa gattaagcgc cgctcacaaa accccacgta gcggtcctgc taggcgcgtg
      121 cagacacggc aaggtcacac cacaacgcca agcggcgcta aacacactca acgcccgcca
      181 ggatgccgcc acaacacatc cgccgcgcca gccgccacat tcaaatgccg cgccagcaca
      241 aacagcacat ccgacaaacg gttcaggtaa tgcagcacat gcggcgacac aatatcctcg
      301 cgcgccagcg tgaccgcctg ccgctcggcc cgacgcgcca ccgcccgccc cacatgggcc
      361 aaggccgccg cccgacaacc gcccggcaaa ataaactcgc gcaaaggcgg caaatccgcg
      421 ttcagcgtgg ccagccagct ctccaggcgc aacacctgaa tctcgccaat cgcctgatac
      481 cccggcacgg caatttcacc gcccagatca aacaaatcat gctgagtatc caccagcgct
      541 gcgcgcgccg cgtctggcaa gggctcacac agcaatacac ccagatggct attgagttcg
      601 tcaacctcgc ccagcaagcg cacgcgcggg caatccttgg ccacccggct gccgtcaccc
      661 aggccggtgc tgccgtcgtc gccggtgcgg gtggtgatgg tggaaagacg atgggtcatg
      721 gtattcactt taaaaaacaa aaattgagtc aagaatgcgc tgggcacatc acgcttgccg
      781 ccaggcagag aggccagcgc tctgattttt atcgggttga ctgtggggca agaccatcct
      841 cagcctcagc tgcgctgagt gctgcaagcc gcgcttaaac gccgtgcacc acacctgccc
      901 agctcgccca gcagcgcggc gcggcgcctg gcgcacacac cgggaacttg gccggcggcg
      961 catcatcaca gcaaaatccg ccctgccacg agccggtgag gcgcgcagca acaccagggc
     1021 aaacctaaga ttcaacatta tccgctatga taaagatcaa taaaaccgct tttttctgga
     1081 gcccacatga ctgacgacct ctcccgcctg agcgaggcgc aatggcgcgc gcggctgacg
     1141 ccagaacaat ttcgcgtgac tcgccagggc ggcaccgaat acccgttcag cggcgaacac
     1201 taccgccgca gcgagcccgg ccaatatcac tgcgtctgct gcggcgccca tctgtttgac
     1261 gctgacgtca aattcgacgc cggctgcggc tggcccagct tctggcaaga agcccccggc
     1321 gccggcatcc gccgcctgcg cgaccgcagc cacggcatga tccgcaccga agtgcgctgc
     1381 ggccagtgcg acgcccacct gggccatgta ttcgacgacg gcccaccgcc taccggcgaa
     1441 cgctactgca tcaactcggt ggcgctcacc ttcaccgccc agagcgccgc atgaatccgc
     1501 ccccactgcc cgccctgcac catccccgcc cctggctgcg cgccctgttt gccatcgccg
     1561 gcgtgctggc cctgctggcc gggatcatcg gcatcttttt gccagtgctg cccaccacgc
     1621 cgtttgtgct gctggccgcc gcctgctttg cccgctcatc gccgcgcata tacggctggc
     1681 tgctccgcca ccgcctgttt ggcccgatga tcgacacctg gcagcgcgaa cgcgccgtgc
     1741 cgcgccgggt aaaatggatt gccctcgcca gcatggcatg ctcgattggc ctgtcgctct
     1801 tgctgatgca aggccaaccc tggctgcaac tgctgctggc cgtgatcggc ctcaccctga
     1861 gcgcctggct gatcaggctg cccgaaccgc aactggccag ggccaaccac gcccagcgct
     1921 agcgccgctc acaaaaccca gcgcagcgtc gcgtggcctg gttgcctgcc tgcggccgca
     1981 cacctgacca gcaccgctgc gctgagtgtg atgagcgacg gtcaaccata cactgagaca
     2041 atcgccaccc ccgcaaagac ggaagcgcaa tcatctgatt ttactgggct ttgtgctccg
     2101 ttttacggct gcaccacaat atcattgcgc accccagcca cccccggcgt cgcccgcgcc
     2161 aaagcctcgg cccgcacccg ctcggccgcg ctgcgggcaa acccggacaa ctgcaccacc
     2221 ccgcgcaaag tctccacccg aatcgccacc gcgctcaccg tcgcatcctc ggcaaaccgc
     2281 gccttcaccc gcgcggtgat gccggtgtcg tccacatact ggccgacggt ggactgatca
     2341 cggctgaccg cgcagccggt aacggacaag gccagcaaag cggccagggc gaaggaggca
     2401 gggagagtgc gacgcatggt gggctcctgg ggaaagtcga agggaaaacg ccccgccgat
     2461 tgggtggcgg ggcgtggggg ttggagtgtg gggggagggg aaggttcgcg ggtcactctg
     2521 cggctgattt tttctcaaga atggcattaa gaattggcgg tctcaaacac caagtgcaac
     2581 agagtcaatg tttaaccgca tgccgtga
//
LOCUS       DAOPYX010000365         2602 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_56197_length_2602_cov_5.716137, whole genome shotgun sequence.
ACCESSION   DAOPYX010000365 DAOPYX010000000
VERSION     DAOPYX010000365.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 2602)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2602)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2602
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56197_length_2602_cov_5.716137"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(135..1370)
                     /locus_tag="PKN55_13425"
     CDS             complement(135..1370)
                     /locus_tag="PKN55_13425"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNX72293.1"
                     /translation="MNVLFIHQNFPGQFKFLAPELARQGHRVVAMSMQEKTPAQWQGV
                     QVVRYGAKRGSTPTIHPWVSDLETKVIRGEACFRAALEMKNQGFVPDVIIAHPGWGES
                     LFLKEVWPHAKLGIYCEFYYHTEGADTGFDPEFPSHDVGLGCRLRLKNTNNLMHFDLA
                     QAGISPTHWQASTFPEPFRSKISVIHDGIDTDAVAPDPDIEVVFNQQLTLTRADEVIT
                     FVNRNLEPYRGYHIFMRALPEILRQRPNARVLLVGGDDVSYGARPDNGRKWKDIFIEE
                     VRGQISDADWARVHFLGNVPYHHFIPLLQLSTVHVYLTYPFVLSWSLLEAMSVGCAIV
                     ASDTLPLHEAIRHDETGRLVDFFDAPALAREVSRLLDAPQERQRLSENARQFARANYD
                     LKTVCLPRQLDWFAALAAA"
     gene            complement(1587..2000)
                     /locus_tag="PKN55_13430"
     CDS             complement(1587..2000)
                     /locus_tag="PKN55_13430"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HNX72294.1"
                     /translation="MPTPSTLFRPLPAPGDIVWCHFPQSLGEPGPKPRPALVVAVSLA
                     DHAVMVVYGTSQKTDKIYPGEFVLDPNDAGFADSGLSHRTKFNLSTQVKLPFSHGWFD
                     QAPGFAGHIPLPKMGVLHPSYMAAAIAASQLCKKS"
     gene            complement(2001..2285)
                     /locus_tag="PKN55_13435"
     CDS             complement(2001..2285)
                     /locus_tag="PKN55_13435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX72295.1"
                     /translation="MDVTAEKHAGSFLLRLRKKDTPTGVSSSTIEALMSLTGLSKTEV
                     THLALRQMADRFLPLYEQDDGALSDEHIRAIRDASSATSIPEERFTERLF"
BASE COUNT          544 a          788 c          752 g          518 t
ORIGIN      
        1 cccgctggtg ccgctgcgcg cgggcgaaac gctggactgg aagctgcaag cctgaggcgc
       61 tgcgcccacc ccataaaaaa cgcccggctc accgggcgtt ttttatgatt gccgcctgtc
      121 cgcgccgcgt gcgctcaggc tgcggccagc gcggcgaacc aatccagctg acgcggcaga
      181 cacacggttt tcaggtcgta attggcgcgg gcaaattgcc gggcgttttc gctcaggcgt
      241 tggcgctctt gcggcgcatc cagcaagcgc gacacctcgc gcgccaaggc cggcgcatca
      301 aaaaagtcca ccaggcggcc ggtctcatca tgccggatcg cttcatgcag cggcagggtg
      361 tcgctggcga caatcgcaca ccccacgctc atcgcctcca gcaagctcca gctcagcaca
      421 aacggatagg tcaggtagac atgcaccgtc gacagctgca agagcgggat gaaatggtga
      481 taaggcacat tgcccagaaa atgcacgcgc gcccaatcgg cgtcgctgat ctggccgcgc
      541 acttcttcga tgaaaatgtc tttccacttg cggccgttat ccgggcgggc gccataactg
      601 acatcgtcgc cccccaccag caacacccgc gcattggggc gctggcgcag gatttccggc
      661 aaggcgcgca taaagatgtg gtagccgcga tagggctcca gattgcgatt gacgaaggtg
      721 atcacctcgt cggcgcgggt cagcgtcagt tgctgattga acaccacttc aatatccggg
      781 tccggcgcca cggcgtcggt gtcgatgcca tcgtgaatca cgctgatttt cgagcggaac
      841 ggctcgggaa aggtgctggc ctgccagtgg gtgggcgaaa tgccggcctg cgccaggtca
      901 aaatgcatca ggttattggt gtttttcaag cgcaaacggc atcccaggcc cacatcatgg
      961 ctgggaaact ccgggtcaaa ccccgtgtcg gcgccttcgg tgtgatagta aaactcgcag
     1021 taaatcccca gcttggcgtg cggccagact tccttgagga acaggctctc cccccagccg
     1081 gggtgggcaa tgatcacatc ggggacaaaa ccctggtttt tcatttccag cgcggcgcga
     1141 aaacacgcct cgccacggat gaccttggtt tccaggtcag acacccaagg gtgaatggtc
     1201 ggcgtagagc cgcgcttggc gccatagcgc accacctgta cgccctgcca ttgcgcaggc
     1261 gttttttctt gcatgctcat cgccacgaca cgatgaccct ggcgcgccag ctctggcgcc
     1321 aggaatttga attgcccagg aaaattctga tggataaaca gtacgttcat agtcacttag
     1381 cccaagaggg ggacacgctc accgcacccg acgccgggcg ctgcgtcaat gcaagccata
     1441 cgcggcggcg cccacgcgcc aacgccgggc agagtatagc ctgatttttc acccctctgg
     1501 catccacgcg ccggccgccg cctaacgatc atggaatgtg gcgtcacggc aaaaccggca
     1561 aggccggcgt ctgccatgcg aaaccttcat gattttttac acagctgact ggcggcaatc
     1621 gccgccgcca tatagctcgg gtggagcacg cccatttttg gcagcgggat atgcccggca
     1681 aaacccggag cctgatcaaa ccacccatga ctgaaaggga gcttgacttg cgtgctcaaa
     1741 ttgaatttgg tgcggtgaga taagccactg tccgcgaagc cggcatcatt tgggtcaagc
     1801 acgaattcac caggatagat tttgtctgtt ttctggctgg tcccgtacac aaccatcacc
     1861 gcatgatctg ccaaagatac ggcaaccacc aaagcgggtc ggggttttgg ccctggctcg
     1921 cccagtgact gagggaagtg acaccaaaca atgtcacctg gcgcggggag cgggcgaaag
     1981 agggtggacg gtgttggcaa ttaaaaaagc ctttccgtga atctttcctc cggaatgctc
     2041 gttgcagaac ttgcgtcacg gatagcccgg atgtgctcgt cgcttaatgc gccatcgtcc
     2101 tgctcgtata gcggcagaaa tctgtccgcc atctggcgca gggccaagtg agtcacttct
     2161 gttttgctca ggccggtgag actcatcaac gcctcaatgg ttgagctgct gaccccagtc
     2221 ggggtgtctt ttttgcgcaa acgcaaaaga aaacttcccg cgtgtttttc ggctgtgacg
     2281 tccatatagg tgtcctactt accatggcgg ccggtcgtgg cgataccagc aaaaacagac
     2341 ttgacgtagt agccagtcac actgaaacac aagggttcat gcgccacgac gaacccctcg
     2401 ccgcaccacg ccgagtcatt cccgcgcagg cgggaatccg ggtgtgtcca cagcgtactg
     2461 cggtctaagc gtcagttcag tcggttcact tcatggtgac tgactactcg cgcaagagcg
     2521 ctgcgctgag tgttgttcgc cgcgcttagc ggtgatttcc gactcaccat gccgctgagc
     2581 cgacttcttt cgtgtatatc aa
//
LOCUS       DAOPYX010000366         2592 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_56555_length_2592_cov_3.650374, whole genome shotgun sequence.
ACCESSION   DAOPYX010000366 DAOPYX010000000
VERSION     DAOPYX010000366.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 2592)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2592)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2592
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56555_length_2592_cov_3.650374"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(209..1171)
                     /locus_tag="PKN55_13440"
     CDS             complement(209..1171)
                     /locus_tag="PKN55_13440"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HNX72296.1"
                     /translation="MTLSKPSPHSLSGASGDRIELLHTFVRIVEAGSLSAAAEQLAAT
                     QPTISRRLQALERQLGVRLLQRTTHSMRLTLDGERCYERARELLSNWASFESDMRGVS
                     DEPEGVLRVIVPHAFGQARLIAPLAQYLLRYPRMQVEWLLHDDRAIQDYIAAGIDCAI
                     QVGEVTDPGLVAIRLSQVPRIVAAAPALLDGAPLPQDPGELAELPWLALRTYYRNEVT
                     LSHADSGKRRRFAIAPRFSTDSLFALRSAMQHGLGVGIASAWVLADDLASGTLVHLAP
                     QWRAAPLPVSLVYPYARFYPARLRRFAELIREGAPAIFGDVAGG"
     gene            1275..2495
                     /locus_tag="PKN55_13445"
     CDS             1275..2495
                     /locus_tag="PKN55_13445"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNX72297.1"
                     /translation="MSLSSSSAAPAAHTDGAHAGPLSASLILLLAISAGLSVASLYYS
                     QPMLGILGADIHAPARLLGQIPTLTQLGYALGLLLLAPLGDRHDRRTVIMVKAGLLTG
                     ALLLAAAAPSIELLLAASLAVGLCATLAQDIVPAAATLAAPHQRGRVVGTVMTGLLLG
                     ILLSRVVSGLVAAQFGWRWMFVLAALSIAGVGLAAWRRLPRFAPGTALPYHALLGSLR
                     TLWHAHAPLRYAAVAQGLLAVGFSAFWSTLAVMLHDSFQLGSAAAGAFGVAGAAGALA
                     APLAGHLADQRGPGAVTRAGAALSTLAFALLTASWLWAPHSWQLGMLAIGALLFDLGV
                     QVTLIAHQTLVYRLDPGARSRLNAVLFVGMFTGMASGAALGSLLLAAWGWAVVMGLAA
                     VTALAAWGVRVRAG"
BASE COUNT          362 a          824 c          914 g          492 t
ORIGIN      
        1 tgatggtgat cggcgtgatg ggggtggtga tgtatgagtt gtttgcgctg gtggaacggc
       61 ggctgacggg gtgggcgcat cggaacccga atgcgccgca ctgaacacgg cgcacaacac
      121 cttaccagcg gacttgtgtt ccaccgtgac tgcccggtca tgtgtttgac cttgccgggc
      181 ggcgtgtgat gcgtggtgct atgcggcatc agcccccggc cacatcgcca aaaatcgccg
      241 gcgcgccctc gcggatcagc tcggcaaagc ggcgcagccg cgccgggtaa aagcgcgcat
      301 acgggtagac caaactcacc ggcagcggcg ctgcacgcca ctgtggcgcc aggtgcacca
      361 gcgtgccgct ggccaggtcg tcggccagca cccaggccga ggcaatgccc acgcccaggc
      421 cgtgttgcat ggcgctgcgc agggcaaaca ggctgtcggt gctaaagcgc ggggcgatgg
      481 caaagcggcg gcgcttgccg ctgtcggcgt ggctgagcgt cacctcgttg cggtaatagg
      541 tgcgcagcgc cagccagggc agttcggcca gctcgccggg gtcttgcggc aagggcgcgc
      601 cgtccagcag cgccggggcg gcggcgacaa tgcgcggcac ctggctcagc cggatggcca
      661 ccaggccagg gtcggtgact tcacccacct ggatggcgca atcaatgccg gcggcgatgt
      721 aatcctgaat cgcccggtca tcatgcaata gccactccac ctgcatgcgc ggatagcgca
      781 acagatactg cgccagcggc gcaatcaagc gcgcctggcc aaaagcatgc ggcacgatca
      841 cccgcaacac gccttccggt tcgtcactga cgccgcgcat atccgattcg aacgacgccc
      901 agttgctcag cagctcgcgg gcgcgctcgt agcagcgctc gccatccagc gtcagccgca
      961 tgctgtgggt ggtgcgttgc aacaggcgca cgcccagttg gcgctccagc gcctgcaagc
     1021 gccggctgat ggttggttgc gtggcggcca gttgctcggc ggcggcagac aggctgccgg
     1081 cttcgacaat gcgcacaaag gtgtgcagca actcgatgcg gtcgccgctg gcgccagaca
     1141 ggctgtgggg gctgggtttg gatagggtca tgcgtgaaac gtatcacagt tgtgcgtctt
     1201 gccccactac gcattcacct ggcgaatggg caagatggcg gccatctatt ccgatgcttc
     1261 tggagcgcct gatcatgtcc ttgtcttctt cctccgccgc accggctgcg cataccgatg
     1321 gcgcccatgc ggggcccttg tccgccagcc tgattttgct gctggccatc agcgccggtt
     1381 tgtcggtggc ctcgctgtac tacagccagc ccatgctggg cattctgggc gccgacattc
     1441 acgctcccgc gcggctattg ggccagattc cgacgctgac gcagctgggc tatgcgctgg
     1501 gcctgttgct gctggccccg ctgggcgacc gccacgaccg ccgcacggtg atcatggtca
     1561 aagccggttt gctgactggt gcgctgctgc tggccgccgc cgcgccgtcg attgagctgt
     1621 tgctggccgc cagcctggcg gtgggcctgt gcgccaccct ggcgcaagac atcgtgccgg
     1681 cggccgccac cctggccgcg ccgcaccagc gcggccgggt ggtgggcacg gtgatgaccg
     1741 ggctgctgct gggcattttg ctgtcgcggg tggtcagcgg cctggtggcg gcgcagtttg
     1801 gctggcgctg gatgtttgtg ctggcggcgc tgagcattgc cggcgtgggc ctggcggcct
     1861 ggcggcgtct gccacgcttt gcccccggca cggccttgcc ctatcacgcc ttgctgggct
     1921 cgttgcgcac gctgtggcac gcccatgccc cgttgcgtta tgcggcggtg gcgcaaggct
     1981 tgctggcggt gggtttcagc gcgttctggt ctaccttggc ggtgatgctg cacgacagtt
     2041 ttcagctggg cagcgccgcg gctggcgcct ttggcgtggc cggcgccgcc ggcgcattgg
     2101 ccgctccgct ggctggccat ctggccgacc aacgcggccc cggcgccgtc acccgcgccg
     2161 gcgcggcgct gagcacgctg gcctttgccc tgctgaccgc cagctggctg tgggcgccgc
     2221 acagctggca actgggcatg ctggccatcg gtgcgctgct gtttgatttg ggcgtgcagg
     2281 tgacgctgat tgcccaccaa accctggtgt atcggctgga ccccggcgcc cgcagccgcc
     2341 tgaatgccgt gctgtttgtg ggcatgttca ccggcatggc cagcggcgcg gctctgggca
     2401 gtttgctgct ggcggcctgg ggttgggcag tggtgatggg gctggcggcg gtaacggcgt
     2461 tggcggcatg gggggtgagg gtgcgggcgg ggtgagttgt ggataaccca aaagggtcgt
     2521 ttttcttcat tgccgcttac atgccaagct caaccgtgtc aatcaattaa ttttaatttt
     2581 cgaaattaat gt
//
LOCUS       DAOPYX010000367         2583 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_56839_length_2583_cov_8.754351, whole genome shotgun sequence.
ACCESSION   DAOPYX010000367 DAOPYX010000000
VERSION     DAOPYX010000367.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 2583)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2583)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2583
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56839_length_2583_cov_8.754351"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(320..1672)
                     /locus_tag="PKN55_13450"
     CDS             complement(320..1672)
                     /locus_tag="PKN55_13450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018150628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008832 - dGTPase activity [Evidence IEA];
                     GO_process: GO:0006203 - dGTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HNX72298.1"
                     /translation="MNPSRMHWTQLLSTQRFQRQGGQIIPQAGGDADAMAGLRTEFHV
                     DHDRVVFSRAFRRLGRKTQVHPLASHDHTHNRLTHSVEVSCVGRSLGNRVGAMLQAGG
                     QLPAGYTPFDIGSVVQVACLAHDIGNPPFGHTGEDALRDWFRQPAHAHYLHGLSEAER
                     CDVQTYEGNAHGLRMVATLEMYADSGGMRLTSAALGSLVKYPWTALGSPRDGKFNIYC
                     AELPYFEAAAEALGLIRLGERRWARHPLSYLMEAADDICYAIVDLEDATEIGILHELE
                     FEKLMSPLIDPARAWSADSPQQRCAAMRGAVVGRCVNEVADRFMAHHDDLLTGQFPAK
                     DLIAVCRAEVRDVLIAAKELASQKIYRHRTKLVTEVAAYPCLAALLDAVMPAVHARVS
                     HGELSPRQQIQLGLLQDVQIGEHDTPYTAYLRVLDWIGAMTDNAAATLAREISGVGIL
                     "
     gene            complement(1815..2462)
                     /locus_tag="PKN55_13455"
     CDS             complement(1815..2462)
                     /locus_tag="PKN55_13455"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014087506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HNX72299.1"
                     /translation="MSTSAPLRLCLVRHGETDWNAARRLQGHLDIPLNPTGHTQARQL
                     IAGLAAHDFAGCLCSDLTRTRQTVAPLLARQPTLTVEYTPALRERHYGCFQGLTPDQA
                     RSQHPQDFALLRSGPADWRPQDGGESFVDHQHRVMACLDALAARAAGQTWLVVTHGGV
                     LDIIYRHVYNLPSSHPRDFLIPNAALNWLRRDADGWQIELWADTRHLQGAALDEI"
BASE COUNT          420 a          827 c          878 g          458 t
ORIGIN      
        1 gctgatcgaa gaatgcggct acggacggcg cttgccggag ctgaaggatg ccgaggcggc
       61 tggctgggcc cagtgataga ggaagattgc ccgccccgcc gctgaccacc cgcccccggc
      121 gggcgatgcc gggttttatg gtctatttgc cccaatcagc aaaaaccccc gcgcgtcacc
      181 cacgggggtt tttgcttggc ccagcagtgg gttgtccgcc tgctgtacgc ggtggacacg
      241 cctggattcc cgcctgcgcg aatgactcgg tggggtgcgg cgaggagttc gaacccttgt
      301 gtttcagcgt gactgcctac tacagaatcc ccaccccaga gatttcccgc gccagggtgg
      361 cggcggcgtt gtcggtcatg gcgccgatcc agtccagcac gcgcagatac gcggtgtaag
      421 gcgtgtcgtg ctcgccaatc tgcacatcct gcaacaagcc cagctggatc tgctggcgcg
      481 ggctgagctc gccgtggctg acgcgggcgt gcaccgccgg catcaccgcg tccagcagcg
      541 ccgccaggca ggggtaggcg gccacttcgg tgaccagttt ggtgcggtgg cggtagattt
      601 tctggctggc cagttctttg gcggcaatca gcacgtcgcg cacctcggcg cggcacacgg
      661 caatcaggtc tttggccgga aactggccgg tgagcaggtc gtcatggtgg gccatgaagc
      721 ggtcggccac ttcgttgacg cagcggccca ccaccgcgcc gcgcatcgcc gcgcaacgct
      781 gctgcgggct gtcggcgctc catgcccgcg ccgggtcgat cagcgggctc atcagttttt
      841 cgaattccag ctcgtgcagg atgccgattt cggtggcgtc ttccagatcg acaatggcgt
      901 agcaaatatc gtcggcggct tccatcagat aagacagcgg gtggcgcgcc cagcggcgct
      961 cgcccaggcg gatcagcccc agcgcctcgg cggcggcctc gaaatacggc agctcggcgc
     1021 agtagatatt gaacttgcca tcgcgcggcg agcccagcgc cgtccacggg tatttcacca
     1081 ggctgcccag cgccgcgctg gtcaggcgca tgccgccgct gtcggcgtac atttccagcg
     1141 tggccaccat gcgcaggccg tgggcgttgc cctcgtaggt ttgcacatcg cagcgctcgg
     1201 cctcgctcag cccgtgcaga taatgcgcgt gcgccggctg gcgaaaccaa tcgcgcaggg
     1261 cgtcttcgcc ggtgtggcca aacggcgggt tgccgatgtc gtgcgccagg caggccacct
     1321 gcaccacgct gccgatgtca aacggcgtat agccggccgg cagctggccg ccggcctgca
     1381 acatggcgcc cacccggttg cccaggctgc ggcccacgca gctgacttcc acgctgtggg
     1441 tgagccggtt gtgggtgtgg tcgtggctgg ccagtgggtg aacctgggtt ttgcgcccca
     1501 gccggcgaaa cgcccgcgag aacaccacgc ggtcgtgatc gacatgaaat tcggtgcgca
     1561 gcccggccat ggcgtcggcg tcgccgccgg cctgcgggat gatctgcccg ccctgacgct
     1621 gaaaacgctg ggtggacagc agttgtgtcc agtgcatgcg gcttgggttc atggtggggc
     1681 tcggcatcaa aatggctcag gctgaggaag gcgctgaaca aagcgtcatt cccgcgcagg
     1741 cgggaatcca gggttttgat ttggcagggt tttatttttg gccaacatgg ttttttgaat
     1801 caacccgcgc atgctcagat ttcatccagc gcggcgccct gcaagtggcg ggtgtcggcc
     1861 caaagctcaa tctgccagcc gtcggcgtcg cggcgcagcc agttcagcgc ggcgttggga
     1921 atcaaaaaat cacgcgggtg gctgctgggc aagttgtaaa catggcgata aataatgtcc
     1981 agcacgccgc cgtgggtgac caccagccag gtttgcccgg ccgcgcgcgc cgccagcgca
     2041 tccaggcagg ccatcacccg gtgttgatga tcgacaaacg attcgccgcc gtcctgcggc
     2101 cgccaatccg ccgggccgct gcgcagcagg gcaaaatctt gcgggtgctg gctgcgcgcc
     2161 tgatccggcg tcagcccctg aaaacagccg tagtggcgct cgcgcagcgc cggggtgtat
     2221 tccaccgtca gcgtgggctg ccgcgccagc agcggggcga cggtttgccg ggtgcgggtt
     2281 agatcgctgc acaggcagcc ggcaaagtca tgcgcagcca ggccggcaat cagctggcgg
     2341 gcctgggtgt ggccggtggg gttcagcgga atgtccagat ggccttgcag ccggcgcgcg
     2401 gcgttccagt cggtttcgcc atggcgcacc agacacaggc gaaggggggc ggatgtgctc
     2461 atacgctgga tgataacaaa cctgaccagc atgccgcacg cgcgccctgc atctggtggg
     2521 catgtcgttt cgctacaaga tcagcgcccg gcctcagcgc taacctatgc cctgacagcc
     2581 gct
//
LOCUS       DAOPYX010000368         2544 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_58184_length_2544_cov_7.900362, whole genome shotgun sequence.
ACCESSION   DAOPYX010000368 DAOPYX010000000
VERSION     DAOPYX010000368.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 2544)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2544)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2544
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58184_length_2544_cov_7.900362"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1039)
                     /locus_tag="PKN55_13460"
     CDS             complement(<1..1039)
                     /locus_tag="PKN55_13460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011136997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HNX72300.1"
                     /translation="MSEFGGMDELLQDFLTESSELLSDVDNKLVELERRPDDKNMLHD
                     IFRGFHTIKGGAGFLNVSPLVTLCHRTENLFDKLRNSEIQLSPEVMDVILEATGVVRD
                     MFDVLRQGRMPESADPMLLEALDRALKADQPITVVQLDPLPTPVAPPPPQPVAPPPAA
                     AAPAPAAAPAPAAAGAPDWQSLYHAVVVPAQAVPAAAPAPVPAAAAPTAPLPVAKPAA
                     PAAPAPRPANKPTAMAPVQQENTIRIDTQRLDQVLNLSGEIGLTKNRLTTLRTEILQG
                     NRDTNTLRSLDEAVSQLDLLVGDLQNAVMKTRMQPIGRLFQKYPRIARDLARQLGKDV
                     ELALVGEETEVD"
     gene            complement(1079..2023)
                     /gene="cheZ"
                     /locus_tag="PKN55_13465"
     CDS             complement(1079..2023)
                     /gene="cheZ"
                     /locus_tag="PKN55_13465"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012696040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0050920 - regulation of chemotaxis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HNX72301.1"
                     /translation="MSDHVQENGDSPELEALFDTLSHKQAAQAAAPAAPVMPPPVGVG
                     DGSGDSAELEALFESVARTAALETPPVTVKPFGDNGDSDELQALFDSLAAAAPEPEVV
                     PPPAPEMPKPDPQVLEMYTRVGQMTRKLHDALHELGYDKSLERAASTIPDAKDRLAYI
                     ASLTENAAMRALNATDQAGPHQDSIEKGATALSLGWDKLYAGQLSVDEFKLLSQHTRD
                     YLASVPSHTQATRTQLMEIIMAQDFQDLTGQVIKKIVEMVQHMESELLAFLMEFSDKK
                     MESKDNGLLNGPVVNAEGRTDVVTSQQQVDDLLESLGF"
     gene            complement(2043..2438)
                     /gene="cheY"
                     /locus_tag="PKN55_13470"
     CDS             complement(2043..2438)
                     /gene="cheY"
                     /locus_tag="PKN55_13470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014088171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HNX72302.1"
                     /translation="MVEADKNTRFLIVDDFSTMRRILRNLLKELGFTNVDEAEDGQAA
                     LHKLRTSHFEFVVSDWNMPNMTGIELLKAVRADNNLKHLPFLMITAEAKRENIIEAAT
                     AGASGYIVKPFTAATLEEKLSKIFANVGK"
BASE COUNT          455 a          738 c          798 g          553 t
ORIGIN      
        1 tgtcgacctc ggtttcttct cccaccaggg ccaattcgac atctttgccc agttgcctgg
       61 ccagatcgcg ggcgattcgc gggtatttct ggaacaggcg gccaatcggc tgcatgcggg
      121 ttttcatcac cgcgttttgc agatcgccca ccagcagatc aagctggctg acagcttcgt
      181 ccagcgagcg cagggtgttg gtgtcgcggt taccttgcag gatttcggtg cgcagcgtgg
      241 tcaggcggtt cttggtcagg ccgatttcac ccgacagatt cagcacctga tccagacgct
      301 gggtgtcgat gcggatggtg ttttcctgtt gcaccggggc catggcagtc ggcttgttgg
      361 ccggacgtgg tgccggagca gccggcgcgg ccggcttggc caccgggagc ggtgctgtcg
      421 gcgcagcggc tgctggcacc ggggcgggag cggcagcagg cacggcttgt gccggcacca
      481 ccacggcgtg atacaggctt tgccagtcag gcgcacccgc cgcagcagga gcaggcgccg
      541 cagcaggagc aggcgccgca gcagcaggcg gcggggcaac cggttgaggc ggcggcgggg
      601 ccacaggggt cggcagcgga tccagttgca cgacggtgat cggctgatca gccttcagtg
      661 cacgatccag tgcttccagc agcatcgggt cggcgctttc cggcatgcgg ccctgacgca
      721 gcacgtcgaa catgtcgcgc accacgccag tggcctcgag aatcacgtcc atgacttccg
      781 gtgaaagctg gatttcgcta ttgcgcagct tgtcgaacag gttttccgtg cggtggcaca
      841 aggtcaccag cgggctgaca ttcaagaaac cggcgccacc cttgatggtg tgaaagcccc
      901 ggaaaatgtc atgcagcatg tttttgtcgt caggacgacg ttccagctcg accagcttgt
      961 tgtccacatc ggacaagagc tcggaagact cggtcaaaaa gtcctgtagc agctcgtcca
     1021 ttccgccgaa ttcgctcatc tttatcctct tgtctctgtg atgcgggcga gcggggcgtt
     1081 aaaagcccag gctttccagc aggtcatcca cttgttgttg gctggtgacg acgtcggtgc
     1141 ggccttccgc gttgaccacc gggccgttga gcaggccgtt gtccttgctt tccatcttct
     1201 tgtcggagaa ttccatcagg aaggccagca gctcgctttc catatgctga accatctcga
     1261 cgattttctt gatcacctgg ccagtcagat cctggaaatc ctgcgccatg atgatttcca
     1321 tcagttgcgt gcgggtcgcc tgggtatgcg aaggcaccga cgccaggtaa tcacgggtgt
     1381 gctgtgacag cagcttgaat tcatccaccg acagctggcc ggcgtagagc ttgtcccatc
     1441 ccagcgacaa ggcggtggcg cctttttcga tcgaatcctg atgcgggcca gcctggtcgg
     1501 tggcattcag cgcacgcatg gcggcgtttt cggtcagcga ggcgatatag gccaggcgat
     1561 ccttggcgtc cggaatggta gaggctgcac gttccagcga cttgtcgtaa cccagttcat
     1621 gcagtgcatc gtgcagcttg cgcgtcatct ggccgacgcg ggtgtacatt tccagcactt
     1681 gcgggtcggg cttgggcatt tcgggggcag gcggcggcac cacttccggc tcgggtgcgg
     1741 cagcagccag actgtcaaac agtgcctgca attcgtcgga atcaccattg tcgccaaacg
     1801 gcttgacggt aaccggcggg gtttccagcg cggcggtgcg cgccacgctt tcaaacaatg
     1861 cttccagctc agctgaatca cccgagccat cacccacccc caccgggggc ggcatcaccg
     1921 gcgctgccgg ggctgcggcc tgagccgcct gcttatgcga caaggtgtcg aacaatgctt
     1981 ccagctccgg cgaatcgcca ttttcctgta cgtggtcgga cacgaggcgt ctcctgctgc
     2041 gattacttgc cgacgttggc gaagatcttg gacagctttt cttccagcgt cgcggcggtg
     2101 aacggcttga cgatgtaacc cgaagcgccg gcagtggccg cttcaatgat gttctcgcgc
     2161 ttggcttcgg ctgtgatcat caggaaaggc aaatgcttca ggttgttgtc ggcacgcacg
     2221 gccttgagca attcgatgcc ggtcatattg ggcatgttcc aatccgacac gacaaattcg
     2281 aagtgcgagg tgcgcagctt gtgcaaggcg gcctggccat cctcggcttc gtccacattg
     2341 gtgaaaccga gctccttgag caggttgcgg aggatgcggc gcatcgtgga aaagtcgtcc
     2401 acgatcagga accgggtgtt tttgtctgct tctaccatgc ttgtagatgt cctgaggaac
     2461 cgatgttgtt taagtgaaag ccgtgattct aaatcagctc acatggaatt atcgttgcaa
     2521 aaatggcagc atggtctctg ccag
//
LOCUS       DAOPYX010000369         2521 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_59041_length_2521_cov_9.203974, whole genome shotgun sequence.
ACCESSION   DAOPYX010000369 DAOPYX010000000
VERSION     DAOPYX010000369.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 2521)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2521)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2521
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59041_length_2521_cov_9.203974"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..1746)
                     /locus_tag="PKN55_13475"
     CDS             complement(1..1746)
                     /locus_tag="PKN55_13475"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNX72303.1"
                     /translation="MKQLTIKALFWAMALASALLAALLALAIYWLGQASDHVAQAERQ
                     RYQSYLLADMLRQSSDDLTRLARTYVVTGEARYEQQYWDILAIRNGEKPMPEAYHRIY
                     WDFVAAGQDKPRPDGVKAPLLELMKQAGFSEAEFAKLNEAKANSDGLVNTETVAMNAV
                     KGRFADGQGGFSREAPPDFELARKLMHDAAYHREKAKIMKPVDDFYLLMDQRTQQAIE
                     IAQTEETRARRFAQAMAALNLLALVAALWGVYRHLLSLLGGEPAQVAARVRGIAAGDL
                     SDADQRAPTGSLLAHVEAMRQSLRQMVALIQSKATDLQTASASLSGATHDIGASAASQ
                     SQSSQSMAQSMDKLSGSIGQISERASGMIDLSSAFGQQASASLQVIDALHEEVGKVSG
                     IASQSSRAVGELDAMSAQIGSMVSIIDEIAEQTNLLALNAAIEAARAGESGRGFAVVA
                     DEVRKLAERTSGSTLEIQRMIQDITRHTREATTLMESQISQVQTGIELAGQARQVVGE
                     IAEKSLDTVESFEHIAHAVQEQSQASQYVRDEIEHVASQNQDTHQAIARARQAADQLH
                     ALASDMHGAVLQFRL"
     gene            complement(2066..2275)
                     /locus_tag="PKN55_13480"
     CDS             complement(2066..2275)
                     /locus_tag="PKN55_13480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX72304.1"
                     /translation="MSALLFLYREVLEINLPWLDQVVRAKRPARLPVVLTRQEVTAVL
                     NRMSGVYGLLAHPAVQKAVAELKAS"
     gene            complement(2288..2356)
                     /locus_tag="PKN55_13485"
                     /pseudo
     CDS             complement(2288..2356)
                     /locus_tag="PKN55_13485"
                     /inference="COORDINATES: protein motif:HMM:NF024886.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
BASE COUNT          422 a          800 c          794 g          505 t
ORIGIN      
        1 ttacagccgg aactgcaaca ccgcgccgtg catatcgctg gccagcgcgt gcagctggtc
       61 ggcggcctgg cgagcgcggg cgatggcttg gtgagtgtcc tggttttggc tggcgacatg
      121 ctcgatttcg tcgcgcacat actggctggc ttgcgattgc tcttgcacgg cgtgggcgat
      181 gtgctcgaac gattccaccg tatccagtga tttttcggca atctcgccca ccacttgccg
      241 cgcctggccg gccagttcga tgccggtttg cacctggctg atttgcgatt ccatcagcgt
      301 ggtggcttcg cgggtgtggc gggtgatgtc ctggatcatg cgctggattt ccagcgtcga
      361 gcccgaggtg cgctcggcca gtttgcgcac ttcgtcggcc accacggcaa agccccggcc
      421 ggattcgccg gcgcgggcgg cctcgatggc ggcgttcagc gccaagagat tggtctgctc
      481 ggcaatctcg tcgatgatgc tgaccatcga gccgatttgc gccgacatgg cgtccagctc
      541 gcccaccgcg cgcgacgatt ggctggcgat gccggacacc ttgcccactt cttcgtgcag
      601 ggcgtcgatc acttgcaggc tggcgctggc ttgctggcca aaggcgctgg acagatcaat
      661 catgccgctg gcgcgctcgg aaatctggcc aatgctgccg gagagtttgt ccatcgactg
      721 cgccatggat tggctggact ggctctggct ggcggccgag gcgccaatat cgtgggtggc
      781 gccagacagc gaggcgctgg ccgtttgcaa atcggtggcc ttgctctgga tcagcgccac
      841 catctggcgc aaggactggc gcatggcttc aacgtgggcc agcaggctgc cggtgggcgc
      901 gcgctggtcg gcgtcgctca ggtcgccggc ggcaatgccg cgcacccgcg ccgccacctg
      961 cgccggctcg ccgcccagca aggacagcaa atgccggtac accccccaca aggccgccac
     1021 cagcgccagc aggttcagcg ccgccatggc ctgggcaaaa cggcgggcgc gggtttcttc
     1081 ggtttgggcg atttcgatgg cctgttgggt gcgctggtcc atcagcagat aaaaatcatc
     1141 caccggcttc atgattttgg ctttttcccg gtggtaggcg gcgtcgtgca tcagcttgcg
     1201 cgccagctcg aaatccggcg gcgcttcacg gctaaagccg ccctggccat cggcaaaacg
     1261 gcctttcacc gcgttcatcg ccaccgtttc ggtgttcacc agcccatcgg agttggcctt
     1321 ggcttcgttc agcttggcga attccgcctc gctgaaaccg gcttgcttca tcagctccag
     1381 caacggcgcc ttgacgccat ccgggcgcgg tttgtcctgg ccggcggcga caaaatccca
     1441 gtaaatgcgg tgatacgcct cgggcatggg tttttcgcca ttgcggatgg cgaggatgtc
     1501 ccagtattgc tgctcgtagc gcgcctcgcc ggtcaccaca taggtgcgcg ccagccgggt
     1561 gaggtcgtcc gagctttgcc gcagcatgtc ggccagcaga taagattgat aacgctggcg
     1621 ctcggcctgc gcgacgtggt cgctggcttg gcccagccag taaatggcca gcgccagcag
     1681 cgccgccaat aatgcagacg ccagcgccat ggcccagaac agggccttga tggtcagttg
     1741 cttcatgagt cccgcctcat tgctgaagat gtcgatgcat cgtcggcgca gggcgggcca
     1801 ccacccggcc ggacgacaag aaaatgtcgc ccacggccgg atctgagtcc gttgatgcgg
     1861 tgagcgcaca gtgtgcgccg ataaaaaatc atcggcacag gctgtgcgct gtgcaattct
     1921 catgccacat aacagaggaa agcgcgggtt tgccgttgtt tggcatacgc tgcgcggtgc
     1981 tgctggccag gtgtgcgggc cgcaggctgt gcggcgggca tgtcaaggtc acgccgccat
     2041 gccagcaagg tgtggcgcgc ggcgtttagc tggctttcag ctcggccacg gccttttgca
     2101 ccgccgggtg agccagcaga ccatacacgc cactcatccg attcagcacc gccgtcacct
     2161 cctgccgcgt cagcaccacc ggcaaccgcg ccgggcgctt ggccctgacc acctgatcca
     2221 gccacggcag attgatctcc agcacctcgc gatagagaaa caacagggct gacaaggctt
     2281 gattctgcgt cgccgcagcc accttgccct cgaccgccag atgcgtcaaa aacgcctcta
     2341 cctccgccgc tcccatctca acggggtgac gcttgtcatg gaacaggata aatctacgca
     2401 cccactggac atactgctgc taggttcgta tgctgtagtg cttgaggcgt agccgcctca
     2461 cgaatctgat caagcagttt gggtggtttt gccggagttg tttgcataat acaaatccat
     2521 t
//
LOCUS       DAOPYX010000370         2506 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_59578_length_2506_cov_15.066911, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000370 DAOPYX010000000
VERSION     DAOPYX010000370.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 2506)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2506)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2506
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59578_length_2506_cov_15.066911"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(2..1723)
                     /gene="dsbD"
                     /locus_tag="PKN55_13490"
     CDS             complement(2..1723)
                     /gene="dsbD"
                     /locus_tag="PKN55_13490"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011135900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047134 - protein-disulfide reductase
                     (NAD(P)) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HNX72305.1"
                     /translation="MSVFFSVWRGWLALLAACLALAAAPALALDPKDLLPPEQAFQVE
                     AQRVGPALRLNIRIADGYYLYRNKLALASAPAGLTAPPAWPLGKRKHDPYFGETEIYR
                     GVFSVDVPLVGPWPDQGRLQLSLQGCADAGICYPPEQRTLTPDSRPASSWLQGGPPPA
                     GQDTALDRLSLPALLASFFVAGLGMALTACMYPMLPIISAIVAGQGARLTRMRGLTLA
                     FAYVQGLAFSYVLVGVAAGLTGSLLTVWLQQPAVILSAAALMVVFALAMFDVIAIQLP
                     SAWQSRLNDASGRLGGGRLASVFAMGALSALIIGPCVAPPLAVALGYIGASGDAWLGG
                     AALYALAMGLGAPLLVVGALGGAVLPRAGAWMNRVKAVFGVVMLALAVYLAAPFLPGW
                     LPLALWAAIALGSALALWRLGGAPARGLALAAAALGLAWLGGAATGATQPLRPWAGLS
                     GAAHAEFPPFTRIADSAELDARLAASQQLVLLDFYADWCVACKEMEAETFPDPAVSAQ
                     LQRFVRLQADVTANRPEHQALLKRFGLFGPPGIIVLTPDGRELHRVVGFTPPADFARQ
                     LAALPSP"
     gene            complement(1723..2067)
                     /gene="cutA"
                     /locus_tag="PKN55_13495"
     CDS             complement(1723..2067)
                     /gene="cutA"
                     /locus_tag="PKN55_13495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008954834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent-cation tolerance protein CutA"
                     /protein_id="HNX72306.1"
                     /translation="MSEKIVLLLCNAPTACAADLARAVVGERLAACVNLLPEVQSVYH
                     WQGQLEEARETPLLCKTTASAAPALMARLAQLHPYDVPEILSWPIADGLPSYLSWVAG
                     EVISSPCERKTV"
     gene            2088..>2506
                     /locus_tag="PKN55_13500"
     CDS             2088..>2506
                     /locus_tag="PKN55_13500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014085726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HNX72307.1"
                     /translation="MRRFAWIVLAFPVIELIGLVLLAGRIGGLATLLWLVASALVGMW
                     ILRNQRLGLLLTAWGALNQGGQMSLYQMLWPLRFLLAGVLLIFPGVISDIIALILLLP
                     LRGPQLGAGQSAPGEGPRAANDSVIEGEFSRVDDPKKR"
BASE COUNT          437 a          875 c          848 g          346 t
ORIGIN      
        1 cttatggcga cggcaaggcc gccagttggc gggcaaaatc ggcaggtggg gtaaagccta
       61 ccacgcgatg cagctcgcgg ccatctggcg tcagcacgat gatgccgggc gggccaaaca
      121 ggccaaagcg cttgagcagg gcctggtgtt ccggccggtt ggcggtgaca tcagcctgca
      181 agcgcacaaa gcgctgcaac tgggcgctga cggctgggtc ggggaaggtt tcggcttcca
      241 tttccttgca ggccacgcac cagtcggcgt agaaatccag caacaccagc tgctggctgg
      301 cggccagccg ggcgtccagc tcggcgctgt cggcaatgcg ggtgaacggc ggaaactcgg
      361 catgcgccgc gccgcttaac ccggcccatg gccgcagcgg ctgggtggcg ccggtggcgg
      421 cgccgcccag ccaggccagg cccagcgcgg cggcggccag cgccagcccg cgcgctggcg
      481 cgccgcccag ccgccacagc gccagcgcgc tgcccagggc aatcgccgcc cacagcgcca
      541 gcggcagcca gccgggcaaa aagggcgcgg ccagatacac cgccagcgcc agcatcacca
      601 cgccaaacac tgccttgacc cggttcatcc acgccccggc gcgcggcagc accgcgccgc
      661 ccagcgcgcc caccaccagc agcggcgcgc ccaggcccat ggccagcgca tacagcgcgg
      721 cgccgcccag ccaggcgtcg ccgctggcgc cgatatagcc cagcgccacc gccagcggcg
      781 gcgccacgca cgggccgata atcagcgccg acagcgcgcc catggcgaac accgacgcca
      841 gccggccgcc gcccagccgc ccggaggcgt cattcaggcg cgattgccag gccgacggca
      901 gctggatggc gatcacgtca aacatcgcca gggcaaacac caccatcagc gcggcggcgg
      961 acaaaatcac cgccggctgt tgcagccaca cggtcagcag gctgccggtc agcccggcgg
     1021 ccacgcccac cagcacatag ctgaacgcca gcccctgcac ataggcaaac gccagcgtca
     1081 gcccgcgcat gcgggtgagc cgcgcgccct ggccggcgac aatcgccgag ataatcggca
     1141 gcatcggata catgcaggcg gtcagcgcca tgcccagccc ggcgacaaaa aaagaagcca
     1201 gcagggctgg caggctcagg cgatccaggg ccgtgtcttg cccggcgggc ggcgggccgc
     1261 cttgcagcca gctgctggcc gggcggctgt ccggcgtcag cgtgcgctgc tcgggcgggt
     1321 agcaaatgcc ggcgtcggcg cagccttgca ggctcagttg caaacggcct tgatccggcc
     1381 acggcccgac cagcggcacg tccacactga acaccccccg gtagatttcg gtttcgccaa
     1441 aatacgggtc gtgcttgcgc ttgcccagcg gccaggccgg cggcgcggtc agcccggccg
     1501 gcgcgctggc cagcgccagc ttgttgcggt acaggtagta gccatcggcg atgcggatat
     1561 tcagccgcaa ggccgggccg acgcgctgcg cctctacctg aaacgcctgc tcgggcggca
     1621 acaggtcttt ggggtcgagc gccagggccg gcgccgccgc cagcgccagg caggcggcga
     1681 gcagcgccag ccagccgcgc catacagaaa aaaacacgga catcacacgg ttttccgttc
     1741 acaggggctt gaaatgacct cacccgccac ccaactcaga taggatggca aaccatcggc
     1801 gatcggccag ctgagaatct ccggcacatc atacggatgc agttgggcca gccgcgccat
     1861 caaggccggc gccgccgagg cggtggtctt gcatagcaac ggggtttccc gcgcctcttc
     1921 cagctggcct tgccagtggt agacagactg cacctcgggc aacaggttca cacaggcggc
     1981 cagccgttcg ccgaccaccg cgcgcgccag atcggcggcg caggccgtgg gcgcgttgca
     2041 caagagcagg acgatttttt cagacatttg gttttcaaga ggctttcatg cgcaggtttg
     2101 cttggattgt actggcattt cccgtcatcg aactgatcgg gctggtgttg ctggccggcc
     2161 gcattggcgg cctggccacc ttgctgtggc tggtggccag cgcgctggtg ggcatgtgga
     2221 ttttgcgcaa ccagcgcctg ggcctgctgc tgaccgcctg gggcgcgctg aaccagggcg
     2281 ggcagatgtc gctgtatcaa atgctgtggc cgctgcgctt tttgctggcc ggggtgttgt
     2341 tgatcttccc tggcgtcatc agcgacatca tcgccttgat tctgctgctg ccgctgcgcg
     2401 gcccgcaact gggcgccggc cagagcgcgc cgggcgaagg cccgcgcgcg gcgaatgatt
     2461 cggtgattga gggcgaattc agccgggtgg atgatccgaa aaaacg
//
LOCUS       DAOPYX010000371         2504 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_59643_length_2504_cov_9.072683, whole genome shotgun sequence.
ACCESSION   DAOPYX010000371 DAOPYX010000000
VERSION     DAOPYX010000371.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 2504)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2504)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2504
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59643_length_2504_cov_9.072683"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            349..909
                     /locus_tag="PKN55_13505"
     CDS             349..909
                     /locus_tag="PKN55_13505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012695543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sua5/YciO/YrdC/YwlC family protein"
                     /protein_id="HNX72308.1"
                     /translation="MLRTLGPSLLQHARARLARGGVLAYPTESCFGLGCHPASATGLR
                     RILALKRRPMHKGMIVVGADWAQLAPYVAPLNREQQARLADYWPGPVTLLLPASRRVL
                     PLLRGRHRKLAVRITAHPETARLCRLLGSALVSTSANLAGQRALKTGRACQQTFGHAV
                     SVLPGHIGKRRKPSTIIDFATGRVLR"
     gene            965..1630
                     /gene="tolQ"
                     /locus_tag="PKN55_13510"
     CDS             965..1630
                     /gene="tolQ"
                     /locus_tag="PKN55_13510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018746300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolQ"
                     /protein_id="HNX72309.1"
                     /translation="MISLIMHASLPVQLIMALLVALSVISWSFIVSKLITLRAVGRST
                     DDFERTFWSGADLNSMYQRVSANQNSMGMEKIFQAGFGEFIKLRRQGGVELSDLMDGT
                     RRAMKAAAQRELDELDRHTAFLATVGSVSPYIGLFGTVWGIMHAFIGLGNAGSATLST
                     VAPGIAEALIATAIGLFAAIPAVVAYNRFAHDTDRFATRFDTFGEEFSNILQRQAARS
                     HSS"
     gene            2114..>2504
                     /locus_tag="PKN55_13515"
     CDS             2114..>2504
                     /locus_tag="PKN55_13515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX72310.1"
                     /translation="MDFVYKSLKYIFYFFLGLGLGFGCRAVSFYSVAGWFLNIFCVFG
                     VCCAKFLARAVFIACGYFCRLSAWDFAGVANFASPDLHQAPGVGVLGYNPAFSLHCPP
                     IMSDPRAIDSFGLYKRLFGYLKQYWRLF"
BASE COUNT          424 a          716 c          730 g          634 t
ORIGIN      
        1 ctggaaatca acccgctggt gttcggtgtt ggcgttggca tgaagttcta agaaccgctc
       61 aaggttcatc cgccgctggc gtggccggcg gcgtgacgag tttttctcgt gtagcaccct
      121 cgtttgacgg cgtatctgtg atgcgccgtt tttttgtggg cggcgcatgc cggcaagttg
      181 gccgcaccat gcgaactttc cccgcgcgtt ccggtctgtc tggccacgcg cagataaagc
      241 acccgcgcac acgccttgcc catcagcgtg tcagtggtgc tttgccgcga taattggcgc
      301 gttcgcgccc gcttctgcca caataccctt ttacctgctt tctgactgat gttgcgcacc
      361 cttggcccct ccctgcttca gcacgcccgc gcccgcctgg cccgtggcgg tgtgctggcc
      421 tatcccaccg aatcctgctt tggcctgggc tgccatccgg cctcggcaac cggcctgcgc
      481 cgcattctgg cgctcaagcg tcggccgatg cacaaaggca tgattgtggt gggggcggac
      541 tgggcgcaac tggcgccgta tgtggcgccg ctcaaccgtg aacagcaggc gcgtctggcc
      601 gattactggc ccggcccagt gactttattg ctgccggctt cccggcgggt gttgccgcta
      661 ttgcgcggcc gtcaccgcaa actggcggtg cgcatcaccg cccatcccga gacggcacgg
      721 ctgtgtcgct tgctgggcag cgcgctggtg tccacctcgg ccaatctggc cggacagcgc
      781 gcgctgaaaa ccggtcgcgc ctgtcagcag acttttggcc atgctgtgtc ggtgttgccc
      841 ggccatatcg gcaaacgccg caaaccttct accatcattg attttgcaac cgggcgcgtg
      901 ttgcgctaac gcccgcccgt tcaacaagga cccagacacc gtggaaagtg gaaattccct
      961 gtcgatgatc tcgctgatca tgcacgccag cctgccggtg cagttgatca tggcgctgtt
     1021 ggtggcgttg tcggtgattt catggagctt tatcgtcagc aagctgatca ccctgcgcgc
     1081 cgtggggcgc agcaccgatg atttcgagcg caccttttgg agcggtgcgg atctgaacag
     1141 catgtaccag cgcgtcagcg ccaaccagaa cagcatgggc atggaaaaaa tcttccaggc
     1201 cggttttggt gaatttatca agttgcgtcg tcagggtggg gtggagttgt ccgacctgat
     1261 ggacggcacc cgccgggcaa tgaaggcggc ggcgcaacgc gagctggacg agctggatcg
     1321 ccacacggcg tttttggcca ccgtaggctc ggtcagcccg tatattggtc tgtttggcac
     1381 ggtgtggggc atcatgcatg cctttattgg cctgggtaac gccggcagcg caaccttgtc
     1441 caccgtggcg cccggcattg ccgaagcgct gatcgccacg gcgattggcc tgtttgccgc
     1501 cattccggca gtggtggcgt ataaccgctt tgcccacgac accgaccgct tcgccacccg
     1561 ctttgacacc tttggcgaag agttctccaa tatcttgcag cgacaagcgg cgcgcagtca
     1621 cagcagctga cccgcacgcc gacgcctttg cgtagcttca cgccaacagc gtttgctgag
     1681 tccttgagcg cggcggagca tgccggaggc gggttgtgag cggctctaaa tagagaaaag
     1741 cccctggtcg ccatgccgcc aggggctttt ctctgatgtc tgggcgatgc acatcgtgca
     1801 tcgccgctac cccactgacc aatccaatac cggagctgct ctcgctgggc agatgccaaa
     1861 ttgtggtatc tgcagattca gcgccacggc cgagggaagg tattgcattg cctgtttttg
     1921 gcgtagtgat tgcagaatat ccatccatgt aaaaatcgtc aatccgtgtt tttacctgtg
     1981 ttcacttgat ttaactttgc gcgccggttg ggttttaagt tttccggcaa aatcggtatc
     2041 tgggcggcaa aaattgccat ttgctccggc aagattgata tacagtaaaa atacttactt
     2101 gcaatgattt ttggtggact ttgtctataa gtcattgaaa tatatttttt atttttttct
     2161 tggtttagga cttggcttcg ggtgtcgtgc cgtgagtttt tattcagttg cgggttggtt
     2221 tttaaatatt ttttgtgtgt tcggtgtgtg ctgcgcaaaa tttcttgcgc gagccgtgtt
     2281 tatcgcttgt ggatattttt gccgcctgtc agcctgggat tttgccgggg tggcaaattt
     2341 tgcctcccct gacttgcatc aagcgccggg cgttggcgtt ttaggctaca atccagcgtt
     2401 ttcattgcat tgccccccca tcatgtctga ccctcgcgcc attgacagtt ttggcctgta
     2461 caagcgtctg tttggctatc tcaagcaata ctggcggtta tttg
//
LOCUS       DAOPYX010000372         2502 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_59720_length_2502_cov_8.065386, whole genome shotgun sequence.
ACCESSION   DAOPYX010000372 DAOPYX010000000
VERSION     DAOPYX010000372.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 2502)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2502)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2502
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59720_length_2502_cov_8.065386"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(242..1312)
                     /gene="modC"
                     /locus_tag="PKN55_13520"
     CDS             complement(242..1312)
                     /gene="modC"
                     /locus_tag="PKN55_13520"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR02142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein"
                     /protein_id="HNX72311.1"
                     /translation="MSQLRARFSLYRPAFSLEVDLHLPGRGVTALFGPSGSGKTTCLR
                     AIAGLERVPGGHVSLGEQVWQDDRHFTPPHQRALGMVFQEASLFAHLNARDNLLFGYR
                     RTPAHERRFGEEEVVELLGIGGLLSRKPHQLSGGERQRVGIGRALLTSPQLLLMDEPL
                     AALDLRRKQEILPYLEKVTTQLEIPVLYVSHAPDEVARLADHMVVLDQGRALASGALN
                     DVLGRVDVPAVFADDAGTVLSVTLAAHEDDALSRLSFAGGDIYVAQQPQPVGAQLRCR
                     VHARDISLSLSAATDDSILNRVTAQIEALAPAPTPGHVLVRLNAGGAPLLARVTQRSA
                     RALNLAPGMTVFAQIKAVALLD"
     gene            complement(1309..1983)
                     /gene="modB"
                     /locus_tag="PKN55_13525"
     CDS             complement(1309..1983)
                     /gene="modB"
                     /locus_tag="PKN55_13525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011137274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HNX72312.1"
                     /translation="MLQDDLQAVWLTLKLASVVTLLLLLIGTPLAWWLARTRSRAREL
                     VAALVAMPLVLPPTVIGFYLLLLMGPHGPVGQAMQALGLPLLPFTFPGVVIASVCYSL
                     PFVVQPIQNAFEAIGERPAEVAATLRASPWDTFWHVMVPLARPGFLTGAILGFAHTIG
                     EFGVVLMIGGNIPGVSRVVSVQIYDHVEAMEYAQAHWLAGGMVAFSLTVLLALYTLNP
                     STRERR"
BASE COUNT          442 a          794 c          834 g          432 t
ORIGIN      
        1 ccagtgaccc cagcattatg agtgctgtgc tctaaccaac tgagctacat agccacgtaa
       61 aagagagcgc aatattacgg gggtgttttt ggcttgtcaa gccttgcgcc gggttttttt
      121 atcaggtgag gccacgtgcg cgccaagcgt gtggatggcg gggctgtggc atgggttctg
      181 ttccgggtga tggcgaatcg cgccatttta agtggcgcga ttcggtggat gcgcgccatg
      241 ctcaatccag cagcgccacc gccttgatct gggcgaatac cgtcataccg ggcgccaggt
      301 tcagcgcgcg cgccgagcgt tgcgtcaccc gcgccaacag cggcgcgccg cccgcgttca
      361 gccgcaccag cacatggcca ggcgttggcg cgggggccag tgcttcgatc tgtgcggtca
      421 cccggttaag aatgctgtcg tcggtggcgg cggacaggct caggctgatg tcgcgggcgt
      481 gcacccggca gcgcagttgg gcgcccaccg gctgtggctg ttgcgccaca taaatatccc
      541 cgccggcaaa gctgagccgg ctgagcgcgt cgtcttcgtg ggcggccagg gtgacgctga
      601 gcaccgtgcc ggcgtcgtcg gcgaacaccg ccggcacatc cacccggccc agcacatcgt
      661 tcagcgcgcc gctggccagc gcccggccct gatccagcac caccatatgg tcggccagcc
      721 gggcgacttc gtctggcgcg tggctgacgt agagcaccgg gatttccagt tgggtggtga
      781 ctttttccag atagggcagg atttcctgtt tgcgccgcaa gtccagcgcc gccagcggtt
      841 cgtccatcag caggagttgc ggcgaggtga gcagcgcgcg gccaatgccc acccgctggc
      901 gctcgccgcc agatagctgg tgcggcttgc gcgacaacag gccgccgatg ccgagcagct
      961 cgaccacttc ttcttcgcca aagcgccgct catgggccgg cgtgcgtcgg tagccaaaca
     1021 gcaaattgtc gcgggcgttc aggtgggcaa acaggctggc ttcctgaaac accatgccca
     1081 gtgcgcgctg gtgcggcggg gtgaagtggc ggtcatcctg ccagacctgc tcgcccaggc
     1141 tgacatggcc gcctggcacg cgctccaggc cggcaatggc gcgcaaacag gtggtcttgc
     1201 ccgagccgga tgggccaaac agtgcggtga cgccacggcc gggcaggtgc agatcaacct
     1261 ccaggctgaa ggccgggcgg tacagggaaa aacgggcgcg cagctggctc atcggcgttc
     1321 tcgtgtgctg gggttcaggg tgtacagcgc cagcagcacg gtcaggctga aggccaccat
     1381 gccgccggcc agccagtgcg cctgggcgta ttccatggct tctacatgat cgtaaatctg
     1441 caccgacacc acccggctga cgccggggat attgccgcca atcatcagca ccacgccaaa
     1501 ttcgccgatg gtgtgggcaa aacccagaat cgccccggtc agaaagcccg gccgcgccag
     1561 cggcaccatc acatgccaga acgtgtccca cgggctggcg cgcagggtgg cggccacttc
     1621 ggccggccgt tcgccaatcg cctcaaacgc attctggatg ggctgcacca caaacggcag
     1681 cgaataacac accgaggcaa tcaccacccc cggaaaggtg aacggcagca gcggcaaccc
     1741 cagcgcctgc atggcctggc ccaccggccc gtgcggcccc atcagcagca agagataaaa
     1801 gccgatcacc gtcggcggca gcaccagcgg catggccacc agcgcggcca ccagttcgcg
     1861 cgcgcgtgag cgggtgcgcg ccagccacca ggccagcggc gtgccgatca gcaacagcaa
     1921 cagggtgacc acactggcca gcttgagcgt cagccagacg gcttgcaggt catcttgcag
     1981 catggcgggc tccggtgcgt acagagaaaa gcgtgaaggt gggcatttgg cgtgatggtg
     2041 taatggatat aaccgggcgt ttagtgccgc tcacaaaacc ctcctggcct tgttgcgcgg
     2101 cctggtcgtc ctgctggtac tgtccgccgc gcgcctggcc aggcgcgctt cgctgagttt
     2161 ggtgagcgac tcttagcaaa tgctgtgccc aagctgggcg gtgcagaatg tctggccagc
     2221 aaagcaggtt tggcgccgtt cagcagccct gcccggcgtt ggcgaagctt tgttgcgttg
     2281 cttgtggcgc ttgcctggcg cgctggctgg cacgctggct ggcgcagtga gtgcgtaata
     2341 tatctgacaa tacgacggta ggtaagacac cagcccattc gggcttgtgt cctggcgctg
     2401 tcggccgcgc agtctggcgg gcgccgcatg tggcgccgct gagcggatta cagctgcttg
     2461 acctcgatat tcaaggtctg gatgcgatag gcaatctgcc gg
//
LOCUS       DAOPYX010000373         2497 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_59891_length_2497_cov_8.907043, whole genome shotgun sequence.
ACCESSION   DAOPYX010000373 DAOPYX010000000
VERSION     DAOPYX010000373.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 2497)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2497)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2497
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59891_length_2497_cov_8.907043"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..321
                     /locus_tag="PKN55_13530"
     CDS             <1..321
                     /locus_tag="PKN55_13530"
                     /inference="COORDINATES: protein motif:HMM:NF016086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-E domain-containing protein"
                     /protein_id="HNX72313.1"
                     /translation="SLSAFENYRKVYRHAITPARVAELLILRGDMPRSLLACLAEVER
                     NLKAVANKQSGETERRAGMMHAELRYGRIDDILHTGMHAYLTDFLERINDLGARISQD
                     FLVG"
     gene            399..>2497
                     /locus_tag="PKN55_13535"
     CDS             399..>2497
                     /locus_tag="PKN55_13535"
                     /inference="COORDINATES: protein motif:HMM:NF021424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HNX72314.1"
                     /translation="MSIHVALRHVTHYRYDRLVNLSPHVVRLRPAPHCRTPILSYSLN
                     IEPAGHFINWQQDPFSNYLARLVFPEKTGEFKITVDLVAEMSVFNPFDFFLEPSAEQF
                     PFAYSPELSHELQPYLAKGELTPALKAFIDGLDLTPVGTVNFLVALNQKIHHAIRYLI
                     RLEPGVQTPEETLTLASGSCRDSGWLLAQVLRHLGLATRFVSGYLIQLKPDVKSLDGP
                     SGAEADFTDLHAWCEVYLPGAGWIGLDPTSGLFAGEGHIPLACSPEPGSAAPVSGAVD
                     KCEVAFEHQMEVSRVLESPRVTLPYSEAQWAAIDALGRQVDADLVAHDVRLTMGGEPT
                     FVADHDRDAEEWNTSAQGPTKRGYATELVQKLCARYGAGGFLHFGQGKWYPGEQLPRW
                     ALSVYWRKDGQPCWRNPALFADERLPSHYTPEDASRFIDTLTRVLGLDTDCILPGYED
                     VWYYLWRERQLPVNVDPFDSRLDDELERERLRRVFKHGLDNAVGYTLPIARDGARWRT
                     GRWFLRDERLYLLPGDSPMGFRLPMDSLPWVDKADYPYLIETDPFAERHALPGAAELR
                     GQFPFALHGASSAAAHGQGVAASSAGHLGDAPQGSAAYNTSEAPARFASAHEVVRTAL
                     CVEARLGVLYVFMPPLAQVEDYLALLAAVETTAESLKVKLVLEGYPPPRDPRLTLLQV
                     TPDPGVIEVNIHPASHW"
BASE COUNT          404 a          884 c          796 g          413 t
ORIGIN      
        1 tcgctgtcgg cgtttgagaa ctaccgcaag gtgtaccgcc acgccatcac cccggcgcgg
       61 gtggcggaat tgctgatttt gcgcggcgac atgccgcgct cgctgctggc ctgcctggcc
      121 gaagtggagc gcaacctcaa ggcggtggcc aataagcaat ccggcgaaac cgaacgccgc
      181 gccggcatga tgcacgccga gctgcgctac ggccgcatcg acgacatcct gcacaccggc
      241 atgcacgctt acctgacaga ttttctggag cgcatcaacg acctgggcgc gcgcatcagc
      301 caggattttc tggtgggctg aacgcccgcg ccgccatgcc ctgaatgacc atcccgtccc
      361 ccgccgccgg cgggggcgct agccctgcgg agagccccat gtccattcac gtcgccctgc
      421 gtcacgtgac ccactatcgc tatgaccggc tggtcaatct gtcgccgcat gtggtgcgcc
      481 tgcggcccgc gccgcactgc cgcacgccga ttttgtccta ttcgctgaac atcgaaccgg
      541 ccggccactt catcaactgg cagcaagacc cgttctccaa ttatctggcc cggctggtgt
      601 tcccggaaaa aaccggcgaa ttcaagatca ccgtcgatct ggtggccgag atgtcggtgt
      661 tcaacccctt tgactttttt ctggagccca gcgccgagca gtttccgttt gcctactcgc
      721 ccgagcttag ccacgagctg caaccctatc tggccaaggg cgagctgacc ccggcgctga
      781 aagcctttat cgacgggctg gacctcaccc cggtgggcac ggtgaacttc ctggtggcgc
      841 tcaaccagaa aatccaccac gccatccgct acctgatccg cctggagccg ggcgtgcaaa
      901 ccccggaaga aaccctcacc ctggccagcg gctcctgccg cgattctggc tggctgctgg
      961 cgcaggtgct gcgccacctg ggcctggcca cccgctttgt gtccggctat ctgatccagc
     1021 tcaagcccga cgtcaaatcg ctggacggcc ccagcggcgc cgaagccgac ttcaccgacc
     1081 tgcacgcctg gtgcgaggtc tacctgcccg gcgccggctg gattggcctg gacccgacct
     1141 cgggcctgtt tgccggcgaa ggccatattc cgctggcctg ctcgcccgaa ccgggcagcg
     1201 ccgcgccagt gagcggcgcg gtggacaagt gcgaagtggc gttcgagcac cagatggaag
     1261 tcagccgcgt gctggaatcc ccgcgcgtca ccctgccgta tagcgaggcg caatgggcgg
     1321 ccatcgacgc cctgggccgc caggtggacg ccgacctggt ggcgcacgat gtgcggctga
     1381 ccatgggcgg cgagcccacg tttgtcgccg accacgaccg cgacgccgaa gaatggaaca
     1441 ccagcgcgca aggcccgacc aagcgcggct acgccaccga gctggtgcaa aagctgtgcg
     1501 cccgctacgg cgctggcggc tttttgcact ttggccaggg caaatggtat ccgggcgagc
     1561 aattgccgcg ctgggcgctg tcggtgtact ggcgcaagga cggccagccg tgctggcgca
     1621 acccggcctt gtttgccgac gaacgcctgc cctcgcacta caccccggaa gacgccagcc
     1681 gctttatcga cacgctgacc cgcgtgctgg gcctggacac cgattgcatc ctgcccggct
     1741 acgaagacgt ctggtattac ctgtggcgcg agcgccagtt gccggtgaac gttgatccct
     1801 tcgactcgcg gctggacgac gaactcgagc gcgagcgcct gcgccgggtg ttcaagcacg
     1861 gcctggacaa cgccgtgggc tacaccctgc cgattgcccg cgatggcgcg cgctggcgca
     1921 ctggccgctg gttcctgcgc gacgagcggc tgtacctgct gcccggcgat tcgccgatgg
     1981 gctttcgcct gccgatggat tcgctgccgt gggtggacaa ggccgactac ccgtatctga
     2041 tcgaaaccga cccgttcgcc gagcgccacg cgttgcctgg cgccgccgag ctgcgcgggc
     2101 agtttccgtt tgccctgcac ggcgccagca gcgccgccgc ccacggccag ggcgtggcgg
     2161 ccagcagcgc cggccatctg ggcgacgcgc cgcagggcag cgccgcctac aacaccagcg
     2221 aagcgccggc gcgctttgcc tccgcccacg aggtggtgcg caccgcgctg tgcgtggaag
     2281 cgcggctggg cgtgctgtat gtgttcatgc cgccgctggc ccaggtggaa gattacctgg
     2341 cgctgctggc ggcggtggaa accaccgccg aatcgctgaa ggtcaaactg gtgctggaag
     2401 gctacccgcc gccgcgcgac ccacggctga ccttgctgca agtcaccccc gaccccggcg
     2461 tgatcgaggt gaacatccac ccggccagcc actggga
//
LOCUS       DAOPYX010000374         2482 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_60431_length_2482_cov_11.533993, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000374 DAOPYX010000000
VERSION     DAOPYX010000374.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 2482)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2482)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2482
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60431_length_2482_cov_11.533993"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2033)
                     /locus_tag="PKN55_13540"
     CDS             complement(<1..2033)
                     /locus_tag="PKN55_13540"
                     /inference="COORDINATES: protein motif:HMM:NF021639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HNX72315.1"
                     /translation="MRAWLTTLTDWLRPERRAARRLPAVLARFHDPRADPLHLARELV
                     DCIRPQQRGDHSALDRYQTMLHTLEADAALRQVFRQRLLALFASRQLLTFFTDSGILP
                     DSGFFSEWLRILGHRLLPARADTHRLNDCLRLIYHHPNDWRWLEAVPPELADRFWAIL
                     AGPGAPAEVDWRGIHTQLLDAVLLLAHRISGLGVDSELMRATPDLERHIPRFTALSAE
                     ALAWARCYRQQDAAGLADAQAQLMVMVAQCEDTLERIRKRARSHGASLRLIYQLTRGQ
                     QSLERLRELVAVLTADLDPARRDDARQFRARFTRNAFIAENRRNSLRHYLQQLSALLA
                     MRVTSHAAETGEHYICANRDEYRQMWRSAGGAGVLIGLMALLKIGAAGLHAPLFVEAL
                     LYSLIYGLGFVLIYLLGLTVATKQPAMTAQTLAAQLAELRPNRQAEVERLVDVAAAVS
                     RSQLAAILGNVLVAAPMALLLSALFTAWHGAPPVDMAKAQHLLADLDPLSWALPHAAI
                     AGFYLFLSGLITGFFANQAAYDDIGARLAQHPGLNRLLGARRAARVGQYVQAHLGGIM
                     GNFLFGCMLGSTGVVGAILGLPLDIRHIAFSSANLGYALAGLEFQLGAAQIAWAVFGV
                     LAIGAVNLAVSFALALRTALRARDAQIGDVWPILRALGHRLRRQPRQFVLPPR"
BASE COUNT          470 a          829 c          816 g          367 t
ORIGIN      
        1 tgtcggggcg gcagcacaaa ctgccggggc tggcggcgca gcctgtgccc cagcgcgcgc
       61 aaaatcggcc agacatcgcc aatttgcgca tcacgcgcgc gcagcgccgt gcgcaaggcc
      121 agggcaaaac tcaccgccag attcaccgcg ccaatcgcca gcacgccaaa caccgcccag
      181 gcaatctgcg ccgcgcccag ctgaaactcc agcccggcca gcgcataccc cagattggcc
      241 gacgagaagg caatatggcg gatatccagc ggcaaaccca aaatcgcccc gaccacgccg
      301 gtcgagccca gcatgcagcc aaacagaaaa ttgcccatga tgccgcccag gtgcgcctgc
      361 acatactggc ccacgcgcgc ggcgcggcgg gcgcccagca ggcgattcag ccccggatgt
      421 tgcgccagcc gggcgccgat gtcgtcgtag gcggcctggt tggcgaaaaa cccagtgatc
      481 agcccggaca aaaacagata aaacccggca atcgccgcgt gcggcagcgc ccagctcaag
      541 gggtcaagat cggccagcaa gtgctgcgcc ttggccatgt ccactggcgg cgcgccatgc
      601 caggcggtga acagcgcgct cagcagcagc gccatcggcg cggcgaccag cacattgccc
      661 aaaatcgccg ccagctggct gcgcgacact gctgcggcca cgtccaccag ccgttcgact
      721 tccgcctggc gattggggcg cagctcggcc aactgggcgg ccagggtttg cgcggtcatc
      781 gccggctgct tggtggccac ggtcaggccg agcagataaa tcagcacaaa gcccaggcca
      841 taaatcaggc tgtacagcag cgcctcgaca aacaagggcg catgcaggcc ggccgcgccg
      901 attttcagca gcgccatcag gccaatcaac acacccgcgc cccctgccga gcgccacatc
      961 tgccggtatt cgtcgcggtt ggcgcagata tagtgctcgc cggtttccgc ggcgtggctg
     1021 gtcactcgca tggccagcag ggcggacaat tgctggagat agtggcgcag gctgttgcgg
     1081 cggttttcgg caataaaagc attgcgggta aagcgcgcgc gaaactggcg ggcgtcgtcg
     1141 cgccgggcgg ggtcaaggtc ggcggtgagc accgccacca gctcgcgcag ccgctccagg
     1201 ctttgctggc ctcgggtgag ctggtaaatc agccgcaggc tggcgccgtg gctgcgcgcg
     1261 cgcttgcgga tgcgctccag ggtgtcttcg cactgcgcca ccatcaccat cagctgcgcc
     1321 tgggcgtcgg ccaggccggc ggcgtcttgc tggcgatagc agcgcgccca ggccagcgcc
     1381 tcggccgaca gcgcggtaaa gcgcgggatg tgccgttcca gatccggggt ggcgcgcatc
     1441 agttcgctgt ccacgcccag cccgctgatg cggtgcgcca gcaacagcac ggcgtccagc
     1501 aattgggtat gaatgccgcg ccaatccacc tcggccggcg cgcccggccc ggccaggatg
     1561 gcccaaaagc ggtcggccag ctcgggcggc acggcttcta gccagcgcca gtcgttgggg
     1621 tggtggtaaa tcagccgcag acagtcgttc agccggtggg tgtcggcgcg cgccggcagc
     1681 aggcggtggc ccagaatgcg cagccattca gaaaaaaacc cgctgtcggg caggatgccg
     1741 ctgtcggtaa aaaaggtcag cagctggcgg ctggcaaaca acgccagcaa acgctggcga
     1801 aacacctggc gcaatgccgc gtcggcttcc agcgtgtgca gcatggtttg atagcgatca
     1861 agagcgctgt gatcgccgcg ctgctgtgga cggatgcaat ccaccagctc acgcgccagg
     1921 tgcagggggt cggcgcgcgg gtcatgaaaa cgcgccagca cggccggcag gcggcgggcg
     1981 gcgcggcgct cggggcgcag ccagtcggtc agtgtggtga gccatgctcg catgaaaact
     2041 ccttgccgag gttcgccgcc tgcctcgcct gatggcaggc aagcggcctg gatgtcttgt
     2101 ccgaccggat agccgccgac aaagtgcccc atgcgcacgg ccatcccgct atgcaatata
     2161 agccattgtc ctgactgggt ggatgccgcc accgcctgaa agccctctga aagcaccgac
     2221 gccgtatgct gcatgcagcg ccaatagggc attttctgcc gttttaagcg tgggtttacc
     2281 gggttatcag ggttttccac tacgggtttt ccgcgattgt tgcccggcgc ggcgcgcact
     2341 accctgcgcc tgggcatcag gcaagggcat gaatcccacc atcaccggca tccgccgaca
     2401 gaagcggagc cattcacgag gggagcaaca ccatgaaaat cggagtcaag ctggcgctgg
     2461 ctcagagcct gctattgctg gc
//
LOCUS       DAOPYX010000375         2477 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_60615_length_2477_cov_10.945087, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000375 DAOPYX010000000
VERSION     DAOPYX010000375.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 2477)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2477)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2477
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60615_length_2477_cov_10.945087"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2198)
                     /locus_tag="PKN55_13545"
     CDS             complement(<1..2198)
                     /locus_tag="PKN55_13545"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004470 - malic enzyme activity [Evidence
                     IEA];
                     GO_function: GO:0004471 - malate dehydrogenase
                     (decarboxylating) (NAD+) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HNX72316.1"
                     /translation="MDEQLRQSALEYHQYPTPGKIQVAPTKPLTTQRDLALAYSPGVA
                     AACDAIVEDPNNAYKYTSRGNLVAVISNGTAVLGLGNIGPLAGKPVMEGKGVLFKKFA
                     GIDVFDIEVNENDPDKLVDIICALEPTFGGVNLEDIKAPECFYIEQKCRERMGIPVFH
                     DDQHGTAIVSAAAVLNSLRLVNKDIDKVRVVASGAGAAAIACLELLVALGVTRANITV
                     CDSKGVIYQGRDEKMDESKKRFAIPDNGQRKLADAMVGADIFMGLSGPKLVTQEMVKS
                     MAAHPLILAMANPEPEILPHLVKEVRPDAIIGTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATTINEEMKLACVRALADLAMAEQNEVVASAYGGDQDLSFGPEYVIPKPFDPRLIVK
                     IAPAVAQAAMDSGVATRPIADMDAYIEQLAQFVYKTNLFMKPVFTQAKKDKKRVVMAE
                     GEDERVLHATQEIASQGLAFPILIGRPSVIEKRIEKLGLKLQAGVDFELVNNESDPRF
                     NDYWTDYYNLMKRKGVSQEQARRRVIGNTTLIGALMVRRGEADAMICGTYGTYRQHFD
                     IVENVIGYAREDKVASAMNALILPTGNIFIADTYVNANPDAGQLAAITEMSAKAVKRF
                     GIAPKVALLSNSSFGTVQSPGAGKMREALAMIQQADPTLEIDGEMQGDAALLETIRLQ
                     AMPSTTLKGAANLLVMPNVEAANISYNLLRVSASDGVTIGPILMGMAKPV"
BASE COUNT          407 a          745 c          802 g          523 t
ORIGIN      
        1 tgcaccggct tggccatgcc catcaggatc gggccaatgg tcacgccgtc cgaggccgac
       61 acgcgcagca ggttgtagct gatgttggcg gcttccacgt tcggcatcac cagcaggttg
      121 gccgcgccct tgagcgtggt gctcggcatg gcttgcaggc ggatggtttc cagcagggcg
      181 gcgtcgccct gcatttcgcc gtcgatttcc agcgtcgggt cggcttgctg aatcatcgcc
      241 agcgcctcgc gcatcttgcc ggcgcccggg ctttgcaccg taccaaagct ggagttggac
      301 agcagcgcca ccttgggggc gatgccaaag cgcttgaccg ccttggccga catctcggtg
      361 atggcggcca gctggccggc gtccgggttg gcgttgacgt aggtgtcggc gatgaagata
      421 ttgccggtcg gcagaatcag cgcattcatc gcgctggcca ccttgtcttc gcgggcgtag
      481 ccgatgacat tttcgacaat gtcaaagtgc tggcgatagg tgccgtaggt gccgcagatc
      541 atcgcgtccg cttcgccacg acgcaccatc agcgcgccaa tcagcgtggt gttgccaatc
      601 acccggcggc gggcctgctc ttgcgagacg cccttgcgct tcatcaggtt gtagtagtcg
      661 gtccagtagt cgttaaagcg cgggtcggac tcgttgttga ccagctcgaa atccacgccg
      721 gcttgcagct tcaggccgag cttttcgatg cgcttttcga tcacgctcgg acggccgatc
      781 agaatcggga aggccaggcc ttgcgaggcg atttcctgcg tggcgtgcag cacgcgctcg
      841 tcttcgcctt cggccatcac cacgcgcttt ttgtctttct tggcctgggt gaacaccggc
      901 ttcatgaaca gattggtctt gtagacaaac tgcgccagct gttcgatata ggcgtccata
      961 tcggcaatcg ggcgggtggc cacgccggaa tccatggcgg cttgcgccac ggccggggcg
     1021 atcttgacga tcaggcgcgg gtcaaacggc ttgggaatca catactcggg gccaaacgac
     1081 agatcctggt cgccgccgta ggccgaggcc accacttcgt tctgctcggc catggccagg
     1141 tcggccaggg cgcgcacgca ggcgagcttc atctcttcgt tgatggtggt cgcgcccacg
     1201 tccagcgcgc cacggaagat gaacgggaag cacagcacat tgttcacctg gttcgggtag
     1261 tccgaacggc cggtgccgat gatggcgtcc gggcgcactt ccttgaccag atgcggcagg
     1321 atttccggct ccggattggc catggccaga atcagcggat gcgcggccat gctcttgacc
     1381 atctcctggg tgaccagctt ggggccggac aagcccatga agatgtcggc gccgaccatg
     1441 gcgtcggcca gcttgcgctg accattgtcg gggatggcga agcgtttttt cgattcgtcc
     1501 atcttttcgt cgcgaccctg gtaaatcacg cccttggaat cacacacggt gatattggcg
     1561 cgggtcacac ccagcgccac cagaagctcc aggcaggcga tggcggcggc gccagcgccg
     1621 gaagccacca cgcgcacctt gtcgatgtct ttgttcacca ggcgcaggct gttgagcacg
     1681 gcggcggcag agacaatggc ggtgccatgc tggtcatcgt ggaacaccgg aatgcccatg
     1741 cgctcgcggc atttttgctc gatgtagaag cactcgggcg ccttgatgtc ttccaggttg
     1801 acgccgccga aagtgggctc cagcgcgcag atgatgtcca ccagcttgtc cgggtcgttt
     1861 tcgttgactt cgatgtcaaa cacatcaatg ccggcaaact tcttgaacag cacgcccttg
     1921 ccttccatca ccggcttgcc ggccagcggg ccgatattgc ccaggcccag cacggcggtg
     1981 ccgttggaaa tcaccgcgac cagattgccg cgcgaggtgt acttgtaagc gttgttcggg
     2041 tcttcgacga tggcgtcgca ggcggcggcc acgcccggcg aatacgccag ggccagatcg
     2101 cgctgggtgg tcagcggctt ggtcggggcc acctggattt tgccgggggt cgggtattga
     2161 tggtattcga gtgcgctctg gcgcagttgc tcatccattg ctggatctcc tgatcaggtg
     2221 cggttcttcg tgtcttggga gttcaggtgt ttcggtcgtg atgcgactct tgatttggcg
     2281 tactgcgtat cgtgtccggt tctgcgggcc agcttgacgc ggggtgggcg tcagccagtg
     2341 cggcgcagtt aaaaggggat gggcaggata agccccagcc cttgcattat aagcgcggat
     2401 gtccgccttg gctcagccgg tcatgcatgg ggccatgcca tgtccgcata acctgcgcac
     2461 aaaacccgcc tggcgtc
//
LOCUS       DAOPYX010000376         2463 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_61140_length_2463_cov_11.771179, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000376 DAOPYX010000000
VERSION     DAOPYX010000376.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 2463)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2463)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2463
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61140_length_2463_cov_11.771179"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..455)
                     /locus_tag="PKN55_13550"
     CDS             complement(<1..455)
                     /locus_tag="PKN55_13550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 family protein"
                     /protein_id="HNX72317.1"
                     /translation="MEYLWPALALLLVLIGLAGTILPALPGLPLLFAGLWLAAWADGY
                     QHVSGATLGWLAGLTALGMAMDALAGWLGARAYGASKQALWGSLIGGVVGLWFGLPGL
                     LLGPLLGAVAGEWLARRDAWRAGQVGMATLVGLALGMLAKIGCALAMLLG"
     gene            598..801
                     /gene="thiS"
                     /locus_tag="PKN55_13555"
     CDS             598..801
                     /gene="thiS"
                     /locus_tag="PKN55_13555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HNX72318.1"
                     /translation="MIQIVLNGEAKTLPAAMTCAGLIAHLSLTGKRVAIERNGDIVPK
                     SRHDQEWLADGDRLELVVAVGGG"
     gene            1046..1837
                     /locus_tag="PKN55_13560"
     CDS             1046..1837
                     /locus_tag="PKN55_13560"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011137313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990107 - thiazole synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HNX72319.1"
                     /translation="MQDPLIIAGREYGSRLLVGTGKYRDFAQTAEALDASGAEIVTVA
                     IRRVNLGQNPDEPNLLDALPKGRYTLLPNTAGCYTADDAVRTLRLARELLDDHRLVKL
                     EVLGDPTSLFPHVTETLKAAETLVKEGFDVMVYTSDDPIVARQLEDIGCCAIMPLASL
                     IGSGMGILNPWNLQLILEQSKVPVLVDAGVGTASDAAIAMELGCQGVLMNTAIAGAQD
                     PIRMAHAMRLGVQAGREAYLAGRMPKKLYHGSPSSPSAGLITAGK"
     gene            2161..2373
                     /gene="rpsU"
                     /locus_tag="PKN55_13565"
     CDS             2161..2373
                     /gene="rpsU"
                     /locus_tag="PKN55_13565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HNX72320.1"
                     /translation="MPSIRVKENEPFEVAMRRFKRSVEKTGLLTELRAREFYEKPTAE
                     RKRKLAAAVKRHYKRLRSQTLPPKLY"
BASE COUNT          454 a          848 c          744 g          417 t
ORIGIN      
        1 cccagcaaca tcgccagcgc gcagccaatc ttggccagca tgcccagcgc cagccccacc
       61 agcgtggcca tgcccacctg gccggcccgc caggcgtcgc gccgcgccag ccattccccc
      121 gccaccgcgc ccagcaaagg ccccagcagc aagccgggca agccaaacca cagccccacc
      181 acgccgccaa tcagcgaccc ccacagcgcc tgcttgctgg cgccataggc gcgcgcgccc
      241 agccagccgg ccagcgcatc catcgccatg cccaaggccg tcagcccggc caaccagccc
      301 agcgtggcgc cggacacatg ctgatagcca tccgcccacg ccgccagcca caggccggca
      361 aacaacagcg gcaggccggg cagggcgggg aggatggtgc cagccaggcc gatgagcacc
      421 agcagcaggg ccaacgcagg ccataggtat tccatggtga cgccctcaag caagcaaatg
      481 ccgattggag cgccagcgcg acggccggtt cccgcaacgc caagcgccgc atgctagaat
      541 ccacgctgca ttccacggcc ggcgtcgccg gcctgatctt ccgggaaaga accactgatg
      601 attcagattg tcctcaatgg cgaggccaaa accctgccgg cggcgatgac gtgcgctggc
      661 ttgattgccc atttgtcgct gaccggcaag cgcgtggcga ttgagcgcaa cggcgacatc
      721 gtgccgaaaa gccgccacga tcaagaatgg ctggccgatg gtgatcggct ggagttggtg
      781 gtggcggtgg gcggcggcta agcgtcgcgc caagcccgct gtggcccagt cgccaatcac
      841 catgatctga accgactgcc gttgcttgga ccgctgtacg ctgtggacac gcctggattc
      901 ccgcctgcgc gggaatgacg cggtgtgatg cggcgcgggg ttcgtcgtgg cgcatgaacc
      961 ctggtatttc aaggtgactg gctatagccg cccgacttta tctgcctgaa ttctgtgtgc
     1021 tgaactgcat tacaaaggac gctccatgca agatccgctt atcatcgccg gccgcgaata
     1081 tggttcgcgc ctgttggttg gcaccggcaa ataccgtgac tttgcccaaa ccgccgaagc
     1141 gctggacgct tctggcgccg aaatcgtcac cgtggcgatt cgccgggtga acctgggcca
     1201 aaacccggac gagcccaacc tgctcgacgc cctgcccaag ggccgctaca ccctgctgcc
     1261 caacaccgcc ggctgctaca ccgccgacga cgccgtgcgc accctgcgcc tggcgcgcga
     1321 gctgttggac gaccatcgcc tggtcaagct ggaagtgctg ggcgacccga cttcgctgtt
     1381 tccgcacgtt accgaaacgc tgaaagccgc cgaaacgctg gtgaaagaag gctttgacgt
     1441 gatggtgtac acctcggacg accccatcgt cgcccgccag ctggaagaca tcggctgctg
     1501 cgccatcatg ccgctggcca gcctgatcgg ctcgggcatg ggcattctca acccgtggaa
     1561 cctgcaactg attctggagc aaagcaaggt gccggtgctg gtggatgccg gcgtgggcac
     1621 cgccagcgat gccgccatcg ccatggagct gggctgccag ggcgtgctga tgaacaccgc
     1681 cattgccggc gcgcaagacc cgattcgcat ggcccacgcc atgcgcctgg gcgtgcaagc
     1741 cggccgcgag gcctatctgg ccgggcgcat gccgaaaaag ctctaccacg gcagccccag
     1801 ctcgccgagc gccgggttga tcaccgccgg caaataagct ttgcggcgat gcgggcggct
     1861 tggccagcgc gcgctgtccg ctgcgtttta ctggccattc gcgcgcccgc tcatgccgtc
     1921 ccgtcgccaa ggcaatcatg gcctgcgcgc gctggccgtg gggcgcggtg cgggtaagct
     1981 cggcggacac gatgcgcgcc cgcctatcct ttctgtcact gtcgccttac cgcgacagcc
     2041 cttgttgtcg cccccttcag cgcatccatc tgttttacaa agccggcttg ctggtttaca
     2101 ctctttggct cagtgtagta ttttttaatt tagataaatc ttaaggattg gtgatttttc
     2161 atgcccagca ttcgcgtgaa agagaatgag ccgttcgaag tggccatgcg ccgtttcaag
     2221 cgctctgttg agaaaactgg cctgctgacc gagctgcgcg cccgcgagtt ctacgagaag
     2281 ccgaccgccg agcgcaagcg caagctcgcc gctgccgtca agcgccacta caagcgcctg
     2341 cgcagccaga ccctgccgcc gaagctgtac tgatctcgac agatcgttcg actgctcagt
     2401 cgcgactgca cgaaaccgcg agcgcaagct tcgcggtttc tctatttctg gcctggcgtt
     2461 tgc
//
LOCUS       DAOPYX010000377         2457 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_61401_length_2457_cov_3.319317, whole genome shotgun sequence.
ACCESSION   DAOPYX010000377 DAOPYX010000000
VERSION     DAOPYX010000377.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 2457)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2457)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2457
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61401_length_2457_cov_3.319317"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..935)
                     /locus_tag="PKN55_13570"
     CDS             complement(<1..935)
                     /locus_tag="PKN55_13570"
                     /inference="COORDINATES: protein motif:HMM:NF024996.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HNX72321.1"
                     /translation="MRNPICLALCLAGASAGALADATIYGTFNAALESVSATGATAGS
                     ASDVRQTQRVTSNTSKIGFKGQEDLGNGVKALWQVEQEVSIDDGGARKGAWAGRNSFV
                     GLEGGFGKVLLGNHDSAYKMFGYLVPLEDGSADFTGKDGLINRVSRLKNSVQYRSPEL
                     AGWLLGASYAADEARTQTSNGQRSNGQVWSLAARYRQDALQAGVAFQRNGEQLSGNSL
                     LANHQDFWKASLRYTLGSTELGVGVEHERMDMARGRDQRQTAWMAAVTQRLGAWALGL
                     AYARLGSRQGADTATVKAADYQADQWALSASYDLST"
     gene            1252..1938
                     /locus_tag="PKN55_13575"
     CDS             1252..1938
                     /locus_tag="PKN55_13575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019923783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNX72322.1"
                     /translation="MRVLLVEDNRPLADWLAKALRQSRFVVDCMHDGADADHVLRTQD
                     YAVVLLDVSLPRMDGWEVLRRLRARGNHAPVLLLTAHGGVEDRVRGLDLGADDYLPKP
                     FELAELEARMRALIRRAAGHDKPALTCGSLCYDSTGRTFALGSQPLSLTPREHAVLEH
                     LMLRSGKPASKEALSEKIFGIDQTASADAIEIYIHRLRKKLDGADVAIVTLRGLGYLL
                     EARQAHAKVA"
     gene            1935..>2457
                     /locus_tag="PKN55_13580"
     CDS             1935..>2457
                     /locus_tag="PKN55_13580"
                     /inference="COORDINATES: protein motif:HMM:NF020110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase N-terminal
                     domain-containing protein"
                     /protein_id="HNX72323.1"
                     /translation="MKRRPASLRGRILLWLLAPLGAMTLVSAWLTYHNAHTLANQAYD
                     RTLQASVRAIAERVRVSDGQVLVDIPLAALEMFEPQFQDRVFYRVSLPRGRVLTGYAD
                     LPAPPPSPRGEIFYFDGVYHGEAVRFAAFYQPLYDPVAQGPVLVQVGETLHSRQQLTR
                     GILWDALGWRLLLA"
BASE COUNT          388 a          833 c          810 g          426 t
ORIGIN      
        1 gtggacaggt catagctggc tgacagcgcc cattggtcag cctgataatc cgccgctttc
       61 accgtggcgg tgtcggcgcc ctggcggctg cccaggcgcg cataggccag acccagtgcc
      121 caggccccca ggcgttgggt gacggcggcc atccaggccg tctggcgctg gtcgcggcca
      181 cgggccatgt ccatgcgctc atgctcgacg cccacgccca gctcggtgct gcccagggtg
      241 tagcgcagcg aggctttcca gaagtcctga tggttggcca gcaggctatt gccgctgagc
      301 tgctcgccat tgcgctggaa ggccacgccg gcttgcagcg catcctgccg gtagcgcgcc
      361 gccagcgacc acacctggcc attgctgcgc tggccgttgc tggtttgcgt gcgcgcctca
      421 tccgccgcat acgaagcgcc cagcagccag ccggccagct ccgggctgcg gtattgcacg
      481 ctgtttttca gccgcgacac gcggttgatc aggccgtcct tgccggtgaa atccgccgag
      541 ccgtcttcca gcggaaccaa atagccaaac atcttgtagg cgctgtcgtg gttgcccagc
      601 agcaccttgc caaaaccgcc ttccaggccg acaaagctgt tgcgcccggc ccatgcgcct
      661 ttgcgcgcgc cgccgtcgtc gatcgagact tcctgctcga cttgccacag cgccttgacg
      721 ccgttgccca ggtcttcctg gcccttgaag ccgatcttgc tggtgttgct ggtgacgcgc
      781 tgggtctggc gcacatcgct ggccgagccg gccgtggcgc cggtggcgct gacgctttcc
      841 agcgcggcgt tgaatgtgcc gtaaatggtg gcgtcggcca gggcgccggc gctggcgccg
      901 gccaggcaca gcgccaggca aatggggttg cgcatggtga aatctcctcg tggtgttgtc
      961 agggttgccg gcaagggcaa cgccgcgcat ggtagggcgc ggcggctttc aggccgcttt
     1021 cagccatgtg gcgcgcgcgc cacgccgtgc gggccggcgc gcggcagaaa accggcgcga
     1081 ccggcccggc ggcgcagcaa aatatcgctt gcttattaaa ttaaacggtt gtttataaat
     1141 cattcccggc tgcattcagc ccgcttgtcg gccaggggga tttctgcgta ggatgaaaaa
     1201 agcgcggcgc acaagaccgc gaaacacaaa acaacaacga ggagacccaa catgcgcgtg
     1261 ctgttggtgg aggacaatcg gccgctggcc gactggttgg ccaaggcctt gcgccaaagc
     1321 cgctttgtgg tggattgcat gcacgacggc gccgacgccg accatgtgct gcgcacccag
     1381 gattacgccg tggtgctgct ggatgtgtcg ctgccgcgca tggacggctg ggaggtgttg
     1441 cgccggctgc gcgcgcgcgg caaccacgcg ccagtgctgc tgctcaccgc ccacggcggc
     1501 gtggaagacc gcgtgcgcgg gctggatctg ggcgcggatg actacctgcc caaacccttc
     1561 gagctggccg agctggaggc gcgcatgcgc gcgctgatcc gccgcgccgc cggccacgac
     1621 aaaccggcgc tcacctgcgg ctcgctgtgc tacgacagca ccgggcgcac ctttgcgctg
     1681 ggcagccagc cgctgtcgct gaccccgcgc gagcacgccg tgctggaaca cctgatgctg
     1741 cgcagcggca agccggccag caaggaagcc ctctcagaaa aaatcttcgg cattgaccaa
     1801 accgccagcg ccgacgccat cgaaatctat atccaccgcc tgcgcaaaaa actggacggc
     1861 gccgacgtgg cgattgtcac cctgcgtggc ttgggctatc tgctggaagc ccgccaagca
     1921 cacgccaagg tggcatgaag cgccgcccgg ccagcctgcg cgggcgcatt ctgctgtggc
     1981 tattggcccc gctgggcgcc atgaccctgg tcagcgcctg gctcacctac cacaacgccc
     2041 acaccctggc caatcaggcc tacgaccgca ccttgcaagc ctcggtgcgc gccatcgccg
     2101 agcgggtgcg cgtcagcgat ggccaggtgc tggtggatat tccgctggcg gcgctggaaa
     2161 tgttcgagcc gcagtttcag gaccgggtgt tttatcgggt gagcctgccg cgcgggcgcg
     2221 tgctcaccgg ctacgccgac ctgccggcac cgccgcccag tccacgcggc gagatttttt
     2281 attttgacgg cgtctaccac ggcgaggcgg tgcgctttgc cgcgttttat caaccgcttt
     2341 acgacccggt ggcgcaaggc ccggtgctgg tgcaagtggg tgaaaccctg cactcgcgcc
     2401 aacagctgac ccgcggcatc ctctgggacg cgctgggctg gcgcttgctg ctggccc
//
LOCUS       DAOPYX010000378         2436 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_62222_length_2436_cov_6.946241, whole genome shotgun sequence.
ACCESSION   DAOPYX010000378 DAOPYX010000000
VERSION     DAOPYX010000378.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 2436)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2436)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2436
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62222_length_2436_cov_6.946241"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(96..2048)
                     /locus_tag="PKN55_13585"
     CDS             complement(96..2048)
                     /locus_tag="PKN55_13585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008953625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-eliminating lyase-related protein"
                     /protein_id="HNX72324.1"
                     /translation="MTASPVCLMVVASHDGRWLHETVQGLNALAARQEALPGLRFLPA
                     ASADEALRLAGEEGDVQGVLVDESMPARGAIALVDALNQLRPELTLYVLLDNDDEKAM
                     VDTLSADSVNGYFFREERDWASWLRIIHAELVDKSATPFFDRLRRYVAMAKDSWHTPG
                     HCGGDSLRASPWVRDFFDFAGEDMLRADLSVSVPMLDSLLHPTGVIHQAQKLAAQAFG
                     ARRTFFATNGTSTANKVIVQTLLAPGDMLLLDRNCHKSVHHAVILSGARPVYLDSSVN
                     RQYGIFGPVPKATLLAAMDAHPQAKALILTSCTYDGFRYDLAPIIEAAHARGIKVIVD
                     EAWYGCARFHPALRPTALECGADYATQSTHKVLSAFSQASMIHVNDPDFNGHLFRENL
                     NMHTSTSPQYGLIASLDVARKQAVMEGYKLLSRALRLAAELREQINATGVFRVLSLEE
                     MLPEDVRDDGVRLDPTKLTVDISASGYSADELREVLFERFNIQVEKSTFNTLTLLVTL
                     GATRSKVSRLYDAMLRLARERRPPAVRQRMPEIPRFTQLACLPRDAFYESGERLPLFD
                     EHGQPNPALVGRVSCDQVTPYPPGIPVLVPGQVIDLSILNYLARLGASQKSIELHGVD
                     ESLGEPALRVLSADELVLLPGGRLDN"
     gene            2255..>2436
                     /locus_tag="PKN55_13590"
     CDS             2255..>2436
                     /locus_tag="PKN55_13590"
                     /inference="COORDINATES: protein motif:HMM:NF019313.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase fold protein"
                     /protein_id="HNX72325.1"
                     /translation="MTMVDHNESVLGDNPTDEDKLGFAEPAARLADKIARFPADTPMV
                     VGVEGKWGEGKSSFLL"
BASE COUNT          525 a          803 c          688 g          420 t
ORIGIN      
        1 gccttggctg gcaaggcttg acgatgcccg tggcgtgaaa gcccgccttg ccggggcact
       61 ttaccagcac gccaggaagg ttttgttagc cgctcttaat tgtccagccg cccgcccggc
      121 aacagcacca gctcatccgc gctgagcacg cgcagcgccg gctcgcccag gctttcatcc
      181 acgccgtgca gctcgatgct tttttgcgaa gcgcccagcc gcgccaggta attcaaaata
      241 gacaaatcaa tcacttggcc cggcaccagc accggaatgc ccggcggata cggcgtcacc
      301 tggtcgcagc tcacccggcc caccagcgcc gggttgggct ggccgtgttc gtcaaacaag
      361 ggcagacgct cgccgctttc gtaaaaagca tcgcgcggca ggcaggccag ttgggtaaag
      421 cgcgggattt ccggcatgcg ctggcgcacc gccggtgggc ggcgttcgcg cgccagccgc
      481 agcatcgcgt catacagccg cgacaccttg ctgcgcgttg cccccagcgt caccagcagg
      541 gtgagggtgt taaaagtcga tttttctact tggatattaa agcgctcgaa caacacctcg
      601 cgcagctcgt cggcgctata accgctggcg gaaatatcca cggtcagctt ggtggggtcc
      661 agccgcacgc cgtcgtcgcg cacatcctcc ggcagcatct cttccaggct gagcacacga
      721 aacaccccgg tggcgttaat ctgctcgcgc agctcggccg ccaggcgcag cgcgcgcgac
      781 aacagcttat aaccctccat caccgcctgc ttgcgcgcca cgtccaggct ggcgatcaag
      841 ccgtattgcg ggctggtgga agtatgcata ttgagatttt cgcggaacaa atggccgtta
      901 aaatccgggt cgttgacatg aatcatgctg gcctgagaaa acgccgacag caccttgtgg
      961 gtggattgcg tggcgtaatc cgcgccacac tccagcgcgg tggggcgcag cgccgggtga
     1021 aagcgcgcgc agccatacca ggcttcgtcc acaatcacct tgatgccgcg cgcatgcgcc
     1081 gcctcgataa tcggcgccag gtcataacga aaaccgtcat aggtgcacga ggtcaaaatc
     1141 agcgccttgg cctgcgggtg agcatccatc gccgccagca gcgtggcctt gggcaccggg
     1201 ccaaaaatcc cgtactgccg attgaccgat gaatccagat acaccggccg cgcgccagac
     1261 aaaatcaccg catggtgcac cgatttatgg caattgcggt ccagcaacaa catatcgccc
     1321 ggcgccagca aggtttgcac aatcacctta ttggcggtgg aggtgccatt ggtggcgaaa
     1381 aatgtccgcc gcgcgccaaa cgcctgcgcc gccagctttt gcgcctggtg aatcaccccg
     1441 gtgggatgca aaagcgaatc cagcatcggc accgacaccg ataaatcggc gcgcagcata
     1501 tcttcgccag caaaatcaaa aaagtcgcgc acccacgggc tggcgcgcag cgaatcacca
     1561 ccgcaatggc cgggggtgtg ccaggaatct ttagccatcg ccacataacg gcgcagccgg
     1621 tcaaaaaacg gcgtggctga tttatccacc agctcggcgt gaataatgcg cagccaactg
     1681 gcccaatcgc gctcttcgcg gaaaaaatag ccatttacgc tatccgccga cagcgtatcc
     1741 accatggctt tttcgtcgtc attgtccagc agcacataca aggtcagctc cgggcgcagc
     1801 tgattgagcg catccaccaa ggcaatcgcc ccgcgcgccg gcatgctctc gtccaccagc
     1861 acaccctgca catcgccctc ttcgccagcc agccgcaacg cctcgtcggc gctggccgcc
     1921 ggcaaaaagc gcaagccggg caaagcctcc tgccgcgccg ccagcgcatt caaaccctgc
     1981 accgtctcat gcagccagcg cccatcatga ctggccacca ccatcaaaca caccggagac
     2041 gctgtcatcc gctcatcctt gtttatctga tccaccggga cgatcacccc ggccatcccc
     2101 agcaggcttc tggcccacca gcccccaccg ccggcaaacc ggcgtttgaa aggggtactg
     2161 tgccagtaaa gggcaaagtc ggaaagggga gcggtgcaag cgctcaccgc gtttggcgta
     2221 caattgaggc caaatgcatg atgaaggcaa aaccatgacc atggtagacc acaacgaatc
     2281 cgtgctgggc gataacccta cagacgaaga caaactcggt tttgccgaac ccgctgcccg
     2341 cctggcggac aaaatcgccc gttttcccgc cgatacaccg atggtggtgg gggtagaggg
     2401 caaatggggt gaggggaagt cgtctttttt gttgat
//
LOCUS       DAOPYX010000379         2436 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_62247_length_2436_cov_2.442671, whole genome shotgun sequence.
ACCESSION   DAOPYX010000379 DAOPYX010000000
VERSION     DAOPYX010000379.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 2436)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2436)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2436
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62247_length_2436_cov_2.442671"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2388
                     /locus_tag="PKN55_13595"
     CDS             <1..2388
                     /locus_tag="PKN55_13595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX72326.1"
                     /translation="GKVMQCAAPDASAHVRDDSQARALAPAGAIADDVAELLDRIMAR
                     LLRKAGARSPAFWRGAATLARAALILADHEVSARRWPGGEAAGALFANTKDGAFDQPL
                     DWHLRTVGARAADFAWRIASLRLPGLATESVEHILSPADERGRFAWQNQAVAAVAALR
                     ERSQGGLLVFNIAATGAGKTIANAKLACTVSRRPRFAIALNLRTLTLQTGDALADDLG
                     LGPDELATVIGDRVASRLHAADPRDEQANAGAFASDGLPTEYDAHGGDMALPEWLGVL
                     TQRRPVLRQVIGAPVLVSTMDYLINAGEPGRQGHHVSALLRMVDSDLVLDEVDSYAPD
                     ALVAVLRVVQSAGLMGRSVICSSATLPQPVAEAVWRAFRSGVQMRCALEERQPRFGVA
                     IVDDQTAPTVLDNEAELPAQFARHVQQLLATPRKAVRCAWVQPVSGRGDEAFVAAIAE
                     AVARLHQAHAWAGPGGKQLSFGLVRVANIGVAIDTARALAQRFPEAWVACYHARDFRI
                     QRHLKEQRLDFLLNRKRGDGHIVADPEIKRLLAASAAASAPFIVVATPVEEIGRDHDF
                     DWGVIEPSSAHSIVQTAGRINRHRLREVSQPNVAILQHNRRWLNGKPGEPCFVWPGLE
                     SESSTHRYASPDLGVLLAEDDLHALDARLRLGGGVMARNEDQIVQRRLATPLDVLEAN
                     ENYPAEWMTQAFYTMYTLRDGQPQQAWRAVQEDGFWVFQRQTRADQQEPWLTRHLGAQ
                     TPPVKNSWLNWRLDELASACVEREIAVTDGLQLQAPYRDEQATLCWDESFGFDWQ"
BASE COUNT          387 a          770 c          865 g          414 t
ORIGIN      
        1 ggcaaggtga tgcagtgcgc tgcgccggat gccagcgctc atgtgcgcga tgacagccag
       61 gcgcgggcgt tggcgccggc cggcgccatt gccgatgacg tggccgagct gctggatcgc
      121 atcatggccc gcttgttgcg caaggccggc gcgcgcagcc cggcgttttg gcgcggcgcg
      181 gccaccctgg cgcgggcggc gttgattctg gccgaccatg aggtgtcggc ccgccgctgg
      241 ccgggcggcg aggcggcggg cgcattgttc gccaacacca aggatggcgc gttcgatcag
      301 ccgctggatt ggcatttgcg caccgtgggc gcacgggcgg cggattttgc ctggcgcatc
      361 gccagcctgc gcttgcccgg cctggccacc gagagcgtgg agcatattct gtcgcctgca
      421 gacgagcgcg ggcgctttgc ctggcaaaat caggcggtgg cggcggtggc cgccttgcgt
      481 gagcgcagcc agggcgggct gctggtgttc aatatcgccg ccaccggcgc aggcaaaacc
      541 atcgccaacg ccaagctggc ctgcacggtg tcgcgccgcc cgcgctttgc catcgccctg
      601 aacctgcgca cgctgacgct gcaaaccggc gacgccctgg ccgacgatct gggcctgggg
      661 ccggatgaat tggccacggt gattggcgac cgtgtagcca gccggctgca tgccgccgac
      721 ccacgcgacg agcaggccaa tgccggcgcc ttcgccagcg atggtctgcc caccgaatac
      781 gatgcccacg gcggtgacat ggcgctgcca gaatggctgg gcgtgctcac ccagcgccgg
      841 ccggtgttgc gccaggtgat tggcgcgccg gtgctggtgt ccaccatgga ttatttgatc
      901 aacgctggcg agccagggcg gcaggggcac catgtgtcgg ccttgctgcg catggtggat
      961 agcgatttgg tgctggatga agtggattcg tacgcgccag acgccttggt ggcggtgctg
     1021 cgtgtggtgc aaagcgccgg gctgatgggg cgcagcgtga tttgctcatc ggccaccttg
     1081 ccgcagccgg tggccgaggc ggtgtggcgg gcgtttcgca gcggcgtgca gatgcgctgc
     1141 gcgctggaag agcggcagcc gcgctttggc gtggccattg tcgatgacca gaccgcgccc
     1201 accgtgctgg acaacgaggc cgaattgccc gcccagtttg cccgccatgt gcagcagttg
     1261 ctggccacgc cgcgcaaggc ggtgcgctgc gcctgggtgc agccggtcag cgggcgtggg
     1321 gacgaagcgt ttgtggcggc gattgccgaa gcggtggccc gcctgcatca ggcgcacgcc
     1381 tgggcggggc cgggcggcaa gcagctgtcg tttgggctgg tgcgggtggc caatatcggc
     1441 gtggccatcg acacggcgcg cgcgctggcg cagcggtttc ccgaggcctg ggtggcgtgt
     1501 tatcacgctc gggattttcg cattcagcgc cacctgaaag aacagcggct ggattttttg
     1561 ctcaaccgca agcgcggcga cgggcatatt gtggccgacc cggaaatcaa acgcttgctg
     1621 gcggccagcg ccgccgccag cgcgccattc atcgtggtgg ccacgcccgt ggaagaaatt
     1681 ggccgcgacc atgattttga ctggggggtg atcgagcctt ccagcgcgca ctccattgtg
     1741 caaaccgccg ggcgcatcaa ccgccatcgc ctgcgcgaag tcagccagcc caatgtggcc
     1801 atcttgcagc acaaccggcg ctggctgaac ggcaaaccag gcgagccttg ttttgtctgg
     1861 ccagggctgg agtcagaatc cagcacacat cgctacgcca gccccgatct gggcgtgttg
     1921 ctggccgagg acgatctaca cgcgctggac gcccgcctgc gcctgggcgg cggggtgatg
     1981 gcgcgcaatg aagaccagat cgtccagcgc cgcctggcca cgccgctgga tgtgctggaa
     2041 gccaatgaaa actatccggc agaatggatg acccaggcgt tttataccat gtacaccctg
     2101 cgcgacggcc agccgcaaca agcctggcga gccgtgcagg aagacggttt ctgggtgttt
     2161 cagcgccaga cccgcgccga ccaacaagag ccctggctca cccgccatct gggcgcgcag
     2221 acgccgccag tcaaaaacag ctggctgaat tggcgtctgg acgaactggc aagcgcctgc
     2281 gtcgagcgcg agattgccgt gaccgatggg ctgcaattgc aagcgcctta ccgcgacgag
     2341 caggctacgc tgtgttggga tgagtcgttt ggctttgatt ggcaataaga gccgctcaca
     2401 aaaccctcct ggcgtcgttg cacggccttg ccgtac
//
LOCUS       DAOPYX010000380         2420 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_62861_length_2420_cov_10.459197, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000380 DAOPYX010000000
VERSION     DAOPYX010000380.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 2420)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2420)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2420
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62861_length_2420_cov_10.459197"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            2..2209
                     /locus_tag="PKN55_13600"
     CDS             2..2209
                     /locus_tag="PKN55_13600"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HNX72327.1"
                     /translation="MVAVTRPLADSLADAADPQHWLASLAPQYTDAEFAMLDKAFRLA
                     REQYQGRTLSHTGEDMFSHAVAAAAIVADLNLLADAVAAALLFATPDLRADSEAWLAR
                     EFNPVVASLVQGCLRVRRIQELGSQHDAATPEARRRQAEALRQMLLAMVADIRVVLIK
                     LAWRTQTMYHLSKVPDDAVRRRVARETLDIFAPLANRLGVWQIKWELEDLAFRHLEPD
                     TYKKIAKLLDERRLDRLSYIEQVLATLRNELAGAGLHADVAGRPKHIYSIWKKMRKKN
                     LDFSELYDIRAVRILVDSIKDCYTALGLIHSLWQPVPGEFDDYINQPKANDYKSLHTA
                     VIGPEDKVVEVQIRTFEMHEHAEFGVAAHWRYKEGGKGDAAYEEKIAWLRQLLDWRED
                     VSDRAGLADAFQTELFADTIYVMTPVGKVLSLPTGATPIDFAYAVHTNIGHRCRGAKV
                     EGQIVPLSTPLQNGQRVEILTVKEGGPSINWLHDGWVVSHRAISKIRQWIRQQNAHIA
                     IDAGRVLFDKELARLPGLQPNLEALAERLNQRGMDEVYAALGHGELSLKALHQALTSF
                     AAPEPAAEPDPESLVRRSRAGHDAGGILIEGVDNLMTVLAKCCKPAPPDPVVGFVTRG
                     RGISIHRVNCRTLKRLSADAPERLIAADWGRQDNSLFAIDVEVVARDRPGLLREFSEV
                     LSRDKVNVTAVHTLSRDAHARMRLTVEVRTVADIQRVLFHLMEVKGVLEARRV"
BASE COUNT          459 a          804 c          770 g          387 t
ORIGIN      
        1 catggttgcc gttacccgtc ccctggccga ctcgctggcc gacgccgccg acccgcagca
       61 ctggctggcc tcactggccc cgcaatacac cgatgccgaa ttcgccatgc tcgacaaggc
      121 gttccggctg gcgcgcgagc aataccaagg ccgcacgctc agccacaccg gcgaagacat
      181 gttcagccac gcggtggcgg cggcggcgat tgtggccgac ctgaacctgc tggccgacgc
      241 ggtggcggcg gcgctgctgt ttgccacgcc agacctgcgc gccgacagcg aagcctggct
      301 ggcgcgcgaa ttcaacccgg tggtggccag cctggtgcaa ggctgcctgc gcgtgcggcg
      361 gattcaagag ctgggcagcc agcacgacgc cgccacgcca gaagcccgcc ggcgccaggc
      421 cgaagccctg cgccagatgc tgctggccat ggtggccgat atccgcgtgg tgctgatcaa
      481 gctggcctgg cgcacgcaaa ccatgtatca cctgtccaaa gtgccggacg atgccgtgcg
      541 gcggcgggtg gcgcgcgaaa cgctggatat ttttgccccg ctggccaacc gcctgggcgt
      601 gtggcaaatc aagtgggagc tggaagacct ggcgtttcgc catctggagc cggacaccta
      661 caaaaaaatc gccaagctgc tggacgaacg ccggctggat cggctcagct atatcgaaca
      721 ggtgctggcc acgctgcgca acgagctggc cggcgccggc ctgcacgccg acgtggccgg
      781 ccggcccaag cacatctaca gcatctggaa aaagatgcgc aaaaaaaacc tggatttttc
      841 cgagctgtac gacatccgcg ccgtgcgcat cctggtggat tcgatcaagg actgctacac
      901 cgcgctgggg ctgattcaca gcctgtggca gccggtgccc ggcgaatttg acgactacat
      961 caaccagccc aaggccaacg actacaaaag cctgcacacc gcggtgattg gcccggaaga
     1021 caaagtggtc gaggtgcaaa tccgcacctt cgagatgcac gagcacgccg aatttggcgt
     1081 ggcggcgcac tggcgctaca aagaaggcgg caagggcgac gccgcctacg aagaaaaaat
     1141 cgcctggctg cgccagctgc tggactggcg cgaagatgtc tccgaccgcg ccggcttggc
     1201 cgacgccttc cagaccgagc tgtttgccga caccatttac gtgatgacgc cagtgggcaa
     1261 ggtgctcagc ctgcccaccg gcgccacgcc gattgatttt gcctacgcgg tgcacaccaa
     1321 tatcggccac cgctgccgcg gcgccaaggt ggaaggacag atcgtgccct tgtccacccc
     1381 cttgcagaat ggccagcggg tggaaatcct caccgtcaag gaaggcgggc cgtcgatcaa
     1441 ctggctgcac gacggctggg tggtcagcca ccgcgccatc agcaagattc gccagtggat
     1501 tcgccagcaa aacgcccata tcgccatcga cgccggccgc gtgctgttcg acaaagaact
     1561 ggcgcgcctg cccggcctgc aacccaatct ggaagcgctg gccgagcggc tgaaccagcg
     1621 cggcatggac gaagtatatg ccgcgctggg ccatggcgaa ttgtcactga aagccctgca
     1681 tcaggcgctg accagctttg ccgcgcccga acccgccgcc gagcccgacc cggaaagcct
     1741 ggtgcgccgc agccgcgccg gccacgacgc tggcggcatc ctgatcgaag gcgtggacaa
     1801 cctgatgacc gtgctggcca agtgctgcaa accggcgccg cccgacccgg tggtgggctt
     1861 tgtcacccgt ggccggggga tttccattca ccgggtgaat tgccgcacgc tcaagcgcct
     1921 gtccgccgac gcccccgagc ggctgatcgc cgccgactgg gggcgccagg acaacagcct
     1981 gttcgccatc gatgtggagg tggtggcgcg cgaccgcccc ggcctgctgc gcgaattctc
     2041 cgaggtgctg tcgcgcgaca aggtgaatgt caccgccgtg cacaccctga gccgcgacgc
     2101 ccacgcccgc atgcggctga ccgtggaagt gcgcacggtg gccgacatcc agcgcgtgct
     2161 gtttcatctg atggaggtca agggcgtgct ggaagcgcga cgggtgtagc cgccgcctgg
     2221 ccgcgcggat tcggtatttt ccggggggcg cgggccgccg gtttgtgcgc agcaacaatt
     2281 tatactgcac tgcacaaaaa atacttgcca aaccgccgcg ccggttcgat aatcaaacct
     2341 acatcaggac gttgcatgca atgtgtgcgt tttggtgttg tctcctccat cctccttcta
     2401 taggtggaat tcagcgcaac
//
LOCUS       DAOPYX010000381         2389 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_64107_length_2389_cov_4.475150, whole genome shotgun sequence.
ACCESSION   DAOPYX010000381 DAOPYX010000000
VERSION     DAOPYX010000381.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 2389)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2389)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2389
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64107_length_2389_cov_4.475150"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1070)
                     /gene="flgK"
                     /locus_tag="PKN55_13605"
     CDS             complement(<1..1070)
                     /gene="flgK"
                     /locus_tag="PKN55_13605"
                     /inference="COORDINATES: protein motif:HMM:TIGR02492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HNX72328.1"
                     /translation="MSSIFGIAVSGLNAARASLTTTSHNISGMNTAGYHRQVNGQASN
                     MANYTGAGYIGNGVQTTSISRVYDSHLEKVVQSNTAMESYYSTQEAQLDQLDGIIADP
                     DVGITPALQSFFESLQVLTQPNMPASIPSRQAVVNQAQALVTRMQTMDARIEEIREGV
                     NTGIRLTVDNINAQANKIAELNKQIQQLTLGDQIVPNDLLDARDQAVLELNKNVKATV
                     VKQDGNVYNVFIGSGQSLVLGEIPMQLAATPSRDDPQRYAVSYKENNMLFEIPSSQID
                     GGSLGGLLNFRRDALDVAQNALNRIAIGFAQSMNAQHLLGVDVNGVPVASKVSSQQEF
                     FAIKSFAADLRVPANSNNTGTL"
     gene            complement(1136..2131)
                     /gene="flgJ"
                     /locus_tag="PKN55_13610"
     CDS             complement(1136..2131)
                     /gene="flgJ"
                     /locus_tag="PKN55_13610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011136427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HNX72329.1"
                     /translation="MQVSARAATTPADISQSYVLDLNAVESLKTSAKHDPKNAIKQVA
                     SQFEAMFLNTLMKTMRQTSFDGEEDSNALDTYQGMFDQQLVQQLAGSGRGTGLAAALE
                     KQLAKAAGVDMGEDAARAGINGQARDMSSMVQTMPPPSWGKQVGTQRSTLETALAKLS
                     EPRVKMEAPAASSASSAPATGGPAEFTKTLLPHAQQAAQELGVAPHLVVAHAALESGW
                     GKKPIRHADGSDSHNLFSIKAGGSWKGKTVDILTTEYVDGAPQKRVERFRAYDNYQDA
                     FADYARLIKNNPRYQGALNQGTDAAGFARGLARGGYATDPNYAAKLSQLAGKSSS"
     gene            complement(2206..>2389)
                     /locus_tag="PKN55_13615"
     CDS             complement(2206..>2389)
                     /locus_tag="PKN55_13615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008953796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HNX72330.1"
                     /translation="NQADIQINQDGGRMINVPKGTNLSQIVKALNAVGATPQDLLAIL
                     QAMKAAGSLKADLQVI"
BASE COUNT          456 a          679 c          684 g          570 t
ORIGIN      
        1 ttcaacgtgc cagtgttatt cgagttggcg ggcacgcgca gatcagccgc aaaactcttg
       61 atggcaaaaa actcttgctg tgaactcacc ttgctggcta ccggcacgcc attcacatcc
      121 acacccagca gatgctgggc attcatggat tgggcaaaac caatggcaat gcggttcagc
      181 gcgttttgcg ccacatccag ggcatcgcgg cggaagttca gcaggccccc cagcgaaccg
      241 ccatcaatct gcgagctggg aatctcaaac agcatattgt tttctttgta cgacaccgca
      301 tagcgttgtg gatcatcacg cgatggcgtc gcagccagtt gcatgggaat ctcacccagc
      361 accagacttt ggccactacc aataaacacg ttgtaaacgt tgccatcctg tttgacgacc
      421 gtggccttaa cgtttttatt cagctccagg accgcttggt cgcgcgcgtc taacaaatcg
      481 ttaggcacga tctgatcacc caaggtcagt tgctggattt gcttgttcag ctcagcaatt
      541 ttgttggcct gggcattgat attgtctacg gtaagccgga taccggtatt gacgccttcg
      601 cggatttctt cgatgcgcgc atccatggtc tgcatgcggg tcaccagcgc ctgcgcttgg
      661 ttgaccacag cctgacggga tggaatgctg gccggcatat tcggctgggt cagtacctgc
      721 aaggattcaa aaaagctttg cagggccggg gtgatgccaa cgtccgggtc ggcaatgatg
      781 ccatccagtt ggtccagctg ggcttcctgg gtggagtaat agctttccat tgctgtattg
      841 gactgcacca ctttttccag gtgggagtca tacacgcggc tgatggaagt ggtttgcacg
      901 ccgttgccga tatagccggc accggtgtag ttagccatat tggatgcctg accattaacc
      961 tgacgatgat agcctgccgt attcatgccg ctgatgttgt ggctggtggt ggtcagcgag
     1021 gcacgggcgg cgttcaggcc gctgacggca attccaaaaa tactcgacat gatgaatccc
     1081 cttgtatctt gtagctatcg accaaaatcc ggtcaacttg atctgtgccg tccagtcaac
     1141 tggacgattt gccggccagt tgcgacagct tggccgcgta gttcggatcg gtggcataac
     1201 caccacgcgc caggccacgg gcaaagccag cggcgtcggt gccttggttg agcgcacctt
     1261 ggtagcgtgg gttgttcttg atcaggcgag cgtaatcggc aaaagcatcc tgatagttgt
     1321 cgtaagcgcg gaaacgctcc acccgctttt gcggcgcgcc atcgacgtat tccgtggtga
     1381 gaatgtccac ggtcttgcct ttccagctac caccggcttt gatgctgaac aagttgtgac
     1441 tgtcgctgcc gtcggcatgg cgaatgggtt ttttgcccca gccgctttcc agtgcggcgt
     1501 gcgccaccac cagatgcggc gccaccccca gctcctgcgc agcttgctgg gcatgcggca
     1561 gcaaagtctt ggtgaactct gccgggccac cggtagccgg tgcactggag gcgctggacg
     1621 cagcaggcgc ttccatcttg acgcgcggct cagaaagctt ggccagtgca gtttccagtg
     1681 ttgagcgctg cgtgccgact tgtttgcccc aactgggtgg cggcatggtc tgcaccatgc
     1741 tgctcatatc gcgcgcctgg ccgttgatgc cggcgcgggc tgcgtcttca cccatgtcca
     1801 cacctgccgc cttggccagc tgtttttcca gtgcggcagc cagcccggtg ccccggccac
     1861 tgcctgccag ttgctggacc aactgctgat cgaacatccc ttgataagta tccagggcat
     1921 tactgtcttc ctcgccatca aacgaggttt gccgcatggt tttcatcagg gtgttgagga
     1981 acatggcctc aaactgcgac gccacctgct tgatggcgtt tttcgggtcg tgcttggccg
     2041 acgtcttgag gctttcaacg gcgttgaggt caagcacata gctttgcgaa atatcagccg
     2101 gcgtggtcgc cgcgcgcgcg cttacttgca tgaaaggcta tcccgttcaa atggcctgac
     2161 atattcagca aggaatatgc caggcctggt tggatcaaat cagggtcaga tcacctgcaa
     2221 atcggccttg agcgaacctg cggctttcat cgcctgcagg atggccagca agtcctgcgg
     2281 ggtggcgccc acggcattca gtgccttgac gatttgcgac agattggtgc ccttgggcac
     2341 attgatcatg cgcccaccgt cttgattgat ctggatatct gcctgattg
//
LOCUS       DAOPYX010000382         2380 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_64511_length_2380_cov_8.424946, whole genome shotgun sequence.
ACCESSION   DAOPYX010000382 DAOPYX010000000
VERSION     DAOPYX010000382.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 2380)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2380)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2380
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64511_length_2380_cov_8.424946"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..279
                     /locus_tag="PKN55_13620"
     CDS             <1..279
                     /locus_tag="PKN55_13620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014085990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HNX72331.1"
                     /translation="FEEFLEAMEDTPALEAFRPQLVYISAGFDAHREDDMGNLGLVDA
                     DYEWVTRQLMRVARTYCEGRIVSVLEGGYDLSALARSVAAHVRVLSAG"
     gene            311..1570
                     /locus_tag="PKN55_13625"
     CDS             311..1570
                     /locus_tag="PKN55_13625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX72332.1"
                     /translation="MVALLALGMLALAPWWLFPAQDAAWRRQQDARALAQAQSALWAW
                     AFAPPLWVNSPHGAETEPGALPYPDRKGLGYSTCTTEGAHLSGSDANLRRLGWLPELG
                     EQARAAMSIPFIANPCDSLTQDAPRDMALAPMLRDGHGQRLWYAVSANVMDRRFEHST
                     RYRQPITLRTLNQTSGWLTVCDARGQVLSDRVAWLLIAAGPPLPGQHRDGSFQRSDYL
                     DRLSFPVGADPRCGGSGRNDDGDNRFVQASPRAGFNDTVLYVEVEHYRVQLVRWITRF
                     LARQLRSHGALPSVRYADVPPAMATPSLLDPDPFPFTSSPWCASLRPDANTVAVKPCQ
                     PSGLSWPAWWAGSYNRNLNDPDRPASVELDTWAASLRLQQDGAAIYVMFDGDPRCWRI
                     SPAATADTALDSQVITYFPPLPSTCPV"
     gene            1622..2380
                     /locus_tag="PKN55_13630"
     CDS             1622..2380
                     /locus_tag="PKN55_13630"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HNX72333.1"
                     /translation="MASSVASAEAGFSLVELAIALAVLGVMLTGLLGPLQQLRTHQRQ
                     QDTRAALAAIRQGLLGYAMSHGRLPCPADPALADSHAQAGLALPDTGMPCQRQAGVLP
                     WKTLGAPALDAWGHRYSYAVAARYSQPVSQLAYPQASSAAELAEAAWYVHLTASATPP
                     AAVQQTRVAALVLSHGANGYGSWDALGQSLPSGSASSGEQLNVVTVGRDLGSNGAPKP
                     FYGPTNPGVDSDDVLEAIPAPLLLHALVSAGRLP"
BASE COUNT          364 a          781 c          787 g          448 t
ORIGIN      
        1 tttgaggaat tcctggaagc aatggaagac acgcctgcac tggaagcctt ccgcccccag
       61 ttggtctata tttcggcagg cttcgacgcc caccgcgaag acgacatggg caacctgggc
      121 ctggtggacg ccgactacga atgggtcacc cgtcagctga tgcgtgtggc gcgcacctac
      181 tgcgaaggcc gcattgtctc ggtgctggag ggcggctacg atctgtccgc gctggcgcgc
      241 agtgtcgccg cccatgtccg ggtgctgtcc gccggttgat ccacgccagc agggcgcggc
      301 cctgttgctg atggtggccc tgctggcgct gggcatgctg gccttggcgc cctggtggct
      361 gtttccggcg caggacgccg cctggcggcg tcagcaggat gcccgtgcgc tggcgcaagc
      421 ccagtcggca ctctgggcgt gggcgtttgc gccgccgctg tgggtgaaca gcccacacgg
      481 cgctgaaacc gagcccggcg cgctgcccta ccccgaccgc aaaggcctgg ggtacagcac
      541 ctgcaccacc gagggcgctc atctgagcgg cagcgatgcc aacttgcgcc gtctgggctg
      601 gctgcccgag ctgggcgagc aggcgcgtgc cgccatgagc attccgttta tcgccaaccc
      661 ttgtgacagc ctgacccagg acgccccgcg cgacatggcg ctggcgccga tgctgcgtga
      721 cggccatggc cagcgcttgt ggtatgcggt gtcggccaat gtgatggacc gacgctttga
      781 acacagcacc cgctatcgcc agcccatcac cttgcgcacc ctcaatcaaa ccagcggctg
      841 gctgaccgtg tgcgatgccc gcggccaggt gttgtctgac cgggtggcct ggttgctgat
      901 cgcggctggc ccgcccttgc ccggacaaca ccgcgatggc agttttcagc gcagtgatta
      961 cctggaccga ctgagttttc cggtgggtgc tgacccgcgt tgcggcggca gcggccgcaa
     1021 cgacgatggc gataaccgct ttgttcaggc cagcccacgc gccggcttta acgacaccgt
     1081 gctgtatgtc gaggtcgagc actatcgggt gcagcttgtc cgctggatca cccgttttct
     1141 ggcccgccaa ttgcgcagcc acggcgcact gcccagcgtg cgctacgccg atgtgccgcc
     1201 ggccatggca acgccttcgc tgctggaccc cgacccgttt ccgtttacct cctcgccgtg
     1261 gtgcgccagc ctgcgcccgg acgccaatac cgtggcggtg aagccttgcc agccctccgg
     1321 cctgagctgg ccggcgtggt gggcgggctc gtataaccgc aacctcaatg acccggatcg
     1381 cccggccagc gttgaactgg acacctgggc ggccagcctg cggctgcagc aggatggcgc
     1441 ggccatctat gtgatgtttg atggtgatcc ccgttgctgg cgcatcagcc ctgccgccac
     1501 tgcggatacg gccttggata gccaagtcat tacgtatttt ccaccattgc cgtcgacatg
     1561 cccggtatag cagactatcc gttctgcaca tgttgcgccg catatctcat gggtgaatgg
     1621 catggcgtca agcgttgcgt cggcagaggc cggcttcagt ctggtggagt tggcaattgc
     1681 cctggcggta ttgggtgtga tgctgaccgg gttattgggc ccgctgcaac agctgcgcac
     1741 ccatcaacgc cagcaggaca cccgtgctgc gctggcggcc attcgccagg gcttgctggg
     1801 ctacgccatg agccatggcc gtttgccctg tccggccgac cctgccttgg cggacagcca
     1861 cgcccaagcc ggattggccc tgccggatac tggcatgccc tgccagcgtc aggccggcgt
     1921 gctgccctgg aagaccctgg gtgcgcccgc gctggatgcc tggggccacc gctacagcta
     1981 tgcggtggct gcacggtaca gccagcctgt cagccaattg gcgtatcctc aggccagcag
     2041 tgcggcagag ttggccgagg ccgcctggta tgtgcatctg actgccagcg ccacgccacc
     2101 ggctgcggtg cagcaaacgc gggtggcggc actggtgttg tcgcatggag ccaatgggta
     2161 tggcagctgg gacgcactgg gacagtcgct gcctagcgga agcgccagca gtggagagca
     2221 gttgaatgtc gtcaccgtgg ggcgcgatct gggcagcaat ggcgcaccca agccgtttta
     2281 tggcccgaca aatcctggcg tggacagtga tgatgtgctg gaagccattc ccgcgccctt
     2341 gctattgcat gcactggtca gcgccggtcg cctgccttga
//
LOCUS       DAOPYX010000383         2352 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_65694_length_2352_cov_13.171963, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000383 DAOPYX010000000
VERSION     DAOPYX010000383.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 2352)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2352)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2352
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65694_length_2352_cov_13.171963"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..384)
                     /locus_tag="PKN55_13635"
     CDS             complement(<1..384)
                     /locus_tag="PKN55_13635"
                     /inference="COORDINATES: protein motif:HMM:NF024093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNX72334.1"
                     /translation="MQFGYTILYVPDVPASLAFYQAAFGLNTRFLHDSGDFAELDTGA
                     TALAFCSHALLHAMGKSPRAPQADHPSFEIALVTDDVPAAVARAQAAGAQLVQAPTDM
                     PWGQTIAYVSDLNGFLVELCTPVSPP"
     gene            complement(494..1840)
                     /locus_tag="PKN55_13640"
     CDS             complement(494..1840)
                     /locus_tag="PKN55_13640"
                     /inference="COORDINATES: protein motif:HMM:TIGR01844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family outer membrane protein"
                     /protein_id="HNX72335.1"
                     /translation="MWTWKHHALALALAGASAGAQALDLIDVWQAAREHDAAYASARA
                     SWEAGQEKTIQGRAPLLPQLNASGDYTRNKAERPAGIGRYRSASAGLQLTQTLFDWSR
                     FAALEQGRQATLIADDQFKNAEQELILRTAQAYFDVLLAEDTLEFTRAAKDAYAKQLE
                     QAKKSFDVGSATITDTYEAQASYDSAVASELAALNDLEVKRNKLALQTGVSANRLDPL
                     KAKVPLELPSPDSADSWVRQAEDGSYALAIRRKQLIVAGKQVDISRSGHLPTVSLVAG
                     YNDRTSTQLASVDTRGGSVGVQVTLPIFSGGLAQSKVREARALEEQARQDLEDARRQV
                     EQATRTAYLGVTSGAAQVTAREQVLASSKSQLDATRLGREVGVRTSVDLLNAERKVYE
                     SRRDLAQARYQYLMARLQLAAAVGQLDRKVLEGVNQLLQARQSTALASAQPKPNKQ"
     gene            complement(1895..>2352)
                     /locus_tag="PKN55_13645"
     CDS             complement(1895..>2352)
                     /locus_tag="PKN55_13645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012698269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNX72336.1"
                     /translation="MLVVVISLLMFQLHSFQRTLLVLLTAPLGMIGVTLTLILFAAPF
                     GFVAMLGVIALAGMIMRNSVILVDQIEHDIREGIDRWEAVVGSTVRRFRPIMLTAAAA
                     ILAMIPLTRSTFWGPMAVAIMGGLLVATVLTLLFLPALYAAWFKVKRPA"
BASE COUNT          347 a          731 c          847 g          427 t
ORIGIN      
        1 cggtgggctc accggcgtgc acagctccac cagaaagcca ttcaggtcgc tgacgtaggc
       61 gatggtctgc ccccagggca tgtccgtcgg cgcttgcacc agctgcgcgc cagcggcttg
      121 ggcgcgggcg acggcggcgg gcacgtcgtc ggtgaccagg gcgatttcaa agctggggtg
      181 gtcggcctgc ggcgcgcgcg gcgatttgcc catggcgtgc agcagcgcgt gcgagcaaaa
      241 cgccagcgcg gtggcgccgg tgtccagctc ggcaaagtcg ccgctgtcgt gcagaaagcg
      301 ggtgttcagg ccaaaggcgg cttgatagaa agccaggctg gccggcacgt cgggcacata
      361 gagaatggtg tagccgaatt gcatgaagct ctccgtcaac aggtggggat gacgcaagcg
      421 tcaccggatt ggggccgcga gagtgggggg cagtggttcc gcgcgtcggg gcttgacgcg
      481 cggggagccg catttattgc ttgttcggct tgggctgggc gctggccagc gcggtgctct
      541 ggcgcgcttg cagcagctgg ttgacgcctt ccagcacctt gcggtccagc tggccgacgg
      601 cggcggccag ttgcaggcgg gccatcaggt attggtagcg cgcttgcgcc aggtcgcggc
      661 gggattcgta caccttgcgc tcggcgttga gcaagtccac gctggtgcgc acgcccactt
      721 cccggcccag gcgggtggcg tccagctggc ttttgctgga ggccagcact tgctcgcggg
      781 cggtgacttg cgccgcgccg ctggtcacgc ccaggtaggc ggtgcgggtg gcttgctcca
      841 cctggcggcg ggcgtcttcc aggtcctggc gggcttgttc ttccagcgcg cgggcttcgc
      901 gcaccttgga ttgcgccagg ccgccgctga aaatcggcag ggtgacttgc acgccgaccg
      961 agccgccgcg cgtgtccacg ctggccagct gagtgctggt gcggtcgttg tagccggcca
     1021 ccaggctgac ggtgggcagg tggccgctgc ggctgatgtc cacttgtttg ccggcgacaa
     1081 tcaattgctt gcggcggatg gccagcgcat agctgccgtc ttcggcctgg cgcacccagc
     1141 tgtcggcgct gtccgggctg ggcagctcca gcggcacttt ggctttcagc gggtccagcc
     1201 ggttggcgct gacgccggtt tgcagcgcca gtttattgcg tttgacttcc aggtcgttga
     1261 gcgcggccag ctcgctggcc acggcggagt cgtaactggc ctgggcttcg taggtgtcgg
     1321 tgatggtggc gctgccgaca tcaaacgatt ttttggcttg ctcaagttgc ttggcgtagg
     1381 cgtctttggc ggcgcgggtg aattccagcg tgtcttcggc cagcagcacg tcaaaatacg
     1441 cctgcgcggt gcgcaggatc agctcttgtt cggcgttttt gaactgatca tcggcaatca
     1501 gcgtggcctg gcggccttgt tccagcgcgg caaagcgcga ccagtcaaac agggtttgcg
     1561 tcagttgcag gccggcgctg gcgctgcggt agcggccgat gccggccggg cgttcggcct
     1621 tgttgcgggt gtagtcgccg ctggcgttca gctgcggcag caagggcgcg cggccctgga
     1681 tggttttttc ctggccggct tcccagctgg cgcgcgccga ggcgtaggcg gcgtcgtgtt
     1741 cgcgcgccgc ctgccagacg tcgatcaggt ccagcgcctg ggcgccggcg ctggcgccgg
     1801 ccagggcgag ggccagcgcg tggtgtttcc atgtccacat gatggatgtc tttccgaagt
     1861 gtgaggggcc cggcgcgcgc cgggccgcgt caggtcaggc tgggcgtttg actttaaacc
     1921 aggccgcgta caacgccggc aagaatagca gcgtcagcac ggtggcgacc agcagccccc
     1981 ccatgatggc caccgccatc ggcccccaga atgtgctgcg ggtcagcggg atcatcgcca
     2041 gaatcgccgc tgccgccgtg agcatgatcg ggcggaaacg gcgcacggtg gagccaacca
     2101 ccgcctccca gcggtcgatg ccttcgcgga tgtcgtgctc gatctggtcc accagaatca
     2161 ccgagttgcg catgatcatc ccggccaggg caatcacccc gagcatggcg acaaagccaa
     2221 acggcgcggc aaacaaaatc agcgtcagcg tcaccccgat catgcccagc ggcgcggtca
     2281 gcagcaccag caaggtgcgc tggaagctgt gcagctggaa catcagcagc gaaatcacca
     2341 ccaccagcat ca
//
LOCUS       DAOPYX010000384         2328 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_66728_length_2328_cov_6.004839, whole genome shotgun sequence.
ACCESSION   DAOPYX010000384 DAOPYX010000000
VERSION     DAOPYX010000384.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 2328)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2328)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2328
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66728_length_2328_cov_6.004839"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..152
                     /locus_tag="PKN55_13650"
     CDS             <1..152
                     /locus_tag="PKN55_13650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HNX72337.1"
                     /translation="SDGVTIGPILMGMAKPVHIVTAISSVRRIVNMVALAAVDAQNQA
                     AQKAE"
     gene            292..534
                     /locus_tag="PKN55_13655"
     CDS             292..534
                     /locus_tag="PKN55_13655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017508908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX72338.1"
                     /translation="MHPDFNPGDPFALFRQFWQAATPPGAQPFLPPMTEEDLDRKLAE
                     LKVVEDWLTMNLGMLHLQVRTLEMQKAARAALKPKE"
     gene            635..1510
                     /locus_tag="PKN55_13660"
     CDS             635..1510
                     /locus_tag="PKN55_13660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018746740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HNX72339.1"
                     /translation="MPTLPRLNLIGPGKVGLALALAWHRAGAVHVAGVLASSADKAQA
                     AVSWLGAGSAVAEAAQLPPAEFTLIATPDGKIADMAARLAAANVTPAGAVVWHASGAL
                     DSAVLAPLAAQGAQCASAHPVMTFADPLRAQDSLPGMPVALEGDVQACARIHALFARV
                     GAQPFSLAAGSKAAYHAALAMASNYLVTLVELASQTLRQAGVAADACPALLAPLMRQS
                     LDNALNMGPLAALTGPIVRGDAHTVAAHLAALASAPERARSYAELGRATLALATPRLN
                     AAQHQALSAALDARS"
     gene            complement(1570..>2328)
                     /locus_tag="PKN55_13665"
     CDS             complement(1570..>2328)
                     /locus_tag="PKN55_13665"
                     /inference="COORDINATES: protein motif:HMM:NF012903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HNX72340.1"
                     /translation="AGPLALPTGALSQAGARIEVVFGSEDDGDGVAGQGTEFNKYYDV
                     VLFDGVNYTSLVTGQTGGTRAAASDLYNTAPEQVVLGNPNSYPKFQTGADINLDFSTN
                     LPPNNIDFGVKFSMTSEAPANGGVLTLEPSKTRSIFDTLNDFSRVLQSSAATPADATD
                     FANRLGNVIANIDNTQTRILSVEARIGANRNEADALVEVGSDFALQYKAILSRLQDVD
                     VASAATELSLAKLALDASQNSFAKVQGLSLFNYI"
BASE COUNT          445 a          744 c          725 g          414 t
ORIGIN      
        1 cctcggacgg cgtgaccatt ggcccgatcc tgatgggcat ggccaagccg gtgcacatcg
       61 tcaccgccat ctcttcggtg cgccgcatcg tcaatatggt ggcgctggcg gcggtggatg
      121 cacagaacca ggcggcacaa aaagctgagt aagcgccgct aagcccccga ccttggtttg
      181 aatgtcgaac caggcgccaa cggcttatac tgaatgggca gacccgccaa gcgggctgcc
      241 catttttcat ggccgccctc gggcgacaca ccaccatacg gggacttcac catgcatcca
      301 gatttcaacc ctggcgaccc gttcgccctg ttccggcaat tctggcaagc cgccaccccg
      361 cccggcgcac agcctttcct gccgccgatg accgaagaag acctcgaccg caaactggcc
      421 gagctgaaag tggtggaaga ctggctgacc atgaacctgg gcatgctgca tctgcaagtg
      481 cgcacgctgg aaatgcaaaa agccgcccgc gccgcgctca agcccaaaga ataaggcctg
      541 aatcggcagc ctctgcccgc ccatgccgcc ccccggcatg ggcggcggtg ttttctggcc
      601 gtatgatctg ggtttttgcc caaggattgc cgccatgccg acattgcccc gtttgaacct
      661 gattggcccc ggcaaggtgg gcctggcgct ggcgctggcc tggcatcgcg caggcgcggt
      721 gcacgtggcc ggtgtgctgg cttcgtccgc cgacaaagcg caggcggcgg tgtcctggct
      781 gggcgccggc agcgcggtgg ctgaggcagc gcaactgccc ccggcagaat tcaccctgat
      841 cgccacccca gacggcaaga ttgccgatat ggcggcgcgc ctggcggcgg caaacgtgac
      901 gccggccggc gcggtggtgt ggcacgccag cggcgcactg gatagcgccg tgctggcccc
      961 gctggcggcg caaggcgcgc agtgcgccag cgcccatccg gtgatgactt ttgccgaccc
     1021 gctacgcgcg caagacagcc tgcccggcat gccggtggcg ctggagggcg acgtgcaggc
     1081 ttgcgcgcgc attcatgcgc tgtttgcccg cgtgggtgcc cagccgttca gcctggccgc
     1141 tggcagcaaa gctgcctacc acgcggcctt ggccatggcg tcgaattatt tggtgacctt
     1201 ggtcgaactg gccagccaga cgctgcgcca ggccggcgtg gcggcagacg cctgcccggc
     1261 gctgctggcg ccgctgatgc ggcaatcgct ggacaatgcg ctgaacatgg ggccgctggc
     1321 ggcgctgacc gggccgattg tgcgcggcga tgcgcacaca gtagccgccc atctggcggc
     1381 gctggccagc gcgcctgagc gggcgcgcag ctacgccgag ttggggcggg cgacgctggc
     1441 gctggccaca ccgcgcctga atgcggcgca acaccaagca ctcagcgcag cactggatgc
     1501 ccggtcataa aaaaaccggc ccgtgcatcc acgcgggccg gttttgctgt gctgctgtcg
     1561 cagtatggct tagatgtaat tgaacaagga caaaccctgc accttggcaa agctgttttg
     1621 cgaagcatcc agcgccagct tggccaaaga cagctcagtg gcggcggagg cgacatccac
     1681 atcttgcaga cggctgagga tggctttgta ttgcagcgca aaatccgagc ccacttccac
     1741 caaggcgtcg gcctcgttgc ggttggcgcc aatccgcgcc tctaccgaca gaatacgggt
     1801 ctgcgtgttg tcgatattgg caatgacatt gcccaggcgg ttggcaaaat cggtagcgtc
     1861 tgccggggta gctgcactgc tttgcaacac gcgggaaaag tcgttcagcg tatcaaagat
     1921 gctgcgcgtc ttgcttggct ccagagtcag tacaccacca ttggccggcg cctcgctggt
     1981 catcgagaac ttcacaccaa aatcaatatt gtttgggggc aggttagtag agaaatccag
     2041 attgatgtca gcgccagtct gaaatttagg gtaggagtta ggattaccca agacaacttg
     2101 ctcaggagcc gtattgtaca gatctgatgc agcagctcgg gtccccccag tttgcccagt
     2161 caccagagag gtgtagttga ctccgtcaaa aagcacaaca tcataatatt tgttgaattc
     2221 agtaccctgg cctgccactc catctccatc atcttccgag ccaaacacca cctcaatgcg
     2281 agcacccgcc tgagacaaag caccggtcgg caaggccaat gggcccgc
//
LOCUS       DAOPYX010000385         2302 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_67882_length_2302_cov_13.319982, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000385 DAOPYX010000000
VERSION     DAOPYX010000385.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 2302)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2302)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2302
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67882_length_2302_cov_13.319982"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            4..780
                     /gene="rsmA"
                     /locus_tag="PKN55_13670"
     CDS             4..780
                     /gene="rsmA"
                     /locus_tag="PKN55_13670"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HNX72341.1"
                     /translation="MSRHIPRKRFGQNFLQDPRTIQDIVDAVHPAPDERVVEIGPGLG
                     AITRPLLARLKHLHVVEIDRDIISRLRGQFSPAQLTIHEGDALAFDFASVGTPMKIVG
                     NLPYNISTPLLFHLAEYGNQVTDMHFMLQKEVVDRMVAEPGTADYGRLSVMLQYRFSM
                     ERILDVPPESFNPPPKVDSAVVRMIPYDTLPHRAQDEALLAHIVQVAFAQRRKTLRNN
                     LKGVLDDDGFTALDIDPGIRPENLSVGGFVAIANYLSKKV"
     gene            1090..1833
                     /locus_tag="PKN55_13675"
     CDS             1090..1833
                     /locus_tag="PKN55_13675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012695517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HNX72342.1"
                     /translation="MIRFVNVHKWFHKLHVLNEVNLQVQAGEVVVVCGPSGSGKSTLI
                     RTVNQLEPISSGEIWVNGVNVSDPKTNLNALRAEVGFVFQHFNLYPHLSVLDNITLAP
                     VQVKKMDRAAANKLGVQLLERVGLAHKQDAFPAQLSGGQQQRVAIARGLAMQPKVMLF
                     DEPTSALDPEMIGEVLKVMKDLAESGMTMMVVTHEMGFAREVADRVVFVDHGQIIEEA
                     PPGEFFTRPQHERAKQFLKQVLSPMHQPE"
     gene            2020..>2302
                     /locus_tag="PKN55_13680"
     CDS             2020..>2302
                     /locus_tag="PKN55_13680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018748110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HNX72343.1"
                     /translation="MRTLTRFAPLAIAAALCAAPALADDLPTLQKIKSSGTIVVGHRE
                     SSIPFSYLDGNQKPVGYSMDLCNKIVEEVKKELKMPALVTKLTPVTSQTR"
BASE COUNT          474 a          760 c          666 g          402 t
ORIGIN      
        1 cccatgtccc gacacattcc ccgcaaacgc ttcggccaga acttcctgca agacccgcgc
       61 accatccagg acatcgtgga cgccgttcac ccggcccccg acgaacgcgt ggtcgaaatc
      121 ggccccggcc tgggcgccat cacccgcccg ctgctggcgc ggctcaagca cctgcacgtg
      181 gtggaaatcg accgcgacat catctcccgc ctgcgcggcc aattcagccc ggcccaactc
      241 accatccacg aaggcgacgc cctggctttc gactttgcct cggtcggcac gccgatgaaa
      301 atcgtcggca acctgcccta taacatctcc accccgctgc tgttccacct ggctgaatac
      361 ggcaatcaag tcaccgacat gcacttcatg ctgcaaaaag aagtggtgga ccgcatggtg
      421 gccgagccgg gcaccgccga ttacggccgg ctgtcggtga tgctgcaata ccgtttttcc
      481 atggagcgca tcctggatgt gccgccggaa tcgttcaacc cgccgcccaa ggtggattcc
      541 gccgtggtgc gcatgatccc ctacgacacc ctgccgcacc gcgcccagga cgaagcgctg
      601 ctggcacata tcgtgcaagt ggccttcgcc caacggcgca aaaccctgcg caacaacctc
      661 aagggcgtgc tggacgacga cggctttacc gcgctggaca tcgaccccgg cattcgcccg
      721 gaaaacctgt cggtgggcgg ttttgtcgcc atcgccaact atctgagcaa aaaggtttaa
      781 aggacacgcg cggcccacaa aacccagcgc agcggtcctg gccaggcgcg cggccgcaga
      841 cagtacaaac agtacggcaa ggccgcgcaa caccgccagg agggttttgt gggccgcgct
      901 attgcggtgc agcttgccaa gccaactgct tagccgcaga atccgagcgt ttttttgcca
      961 ggggacgatc cccccggcaa atcgcttcta aactccaaga cggcgcgcgc ctaaaccccg
     1021 cgcagcgcgg acagcgccgc gccagcacgc caagcaggcg tggcaagcgg cgctcagcaa
     1081 gggaaaacca tgattcgttt cgtaaatgtg cataagtggt ttcataaact gcatgtgctc
     1141 aatgaggtca acctccaggt gcaagcgggt gaggtggtgg tggtgtgcgg gccgtccggc
     1201 tcgggcaaat ccacgctgat tcgcaccgtc aatcagctgg agccgatttc ctctggggaa
     1261 atctgggtca acggcgtcaa tgtgagcgac ccgaaaacca atctgaacgc cctgcgcgcc
     1321 gaagtcggtt ttgtgtttca gcatttcaat ctgtatccgc atctgtcggt gctggacaac
     1381 atcaccctgg cgccggtgca ggtgaaaaaa atggaccgcg ccgccgccaa caagctgggc
     1441 gtgcagctcc tggagcgcgt cggcctggcg cacaagcagg atgcttttcc ggctcaattg
     1501 tctggcggcc agcagcagcg cgtggcgatt gcccgtggcc tggccatgca gcccaaggtg
     1561 atgctgtttg acgagcccac cagcgcgctg gacccggaaa tgatcggcga agtgctgaaa
     1621 gtgatgaaag acctggctga atccggcatg accatgatgg tggtcaccca cgaaatgggc
     1681 tttgcccgcg aagtggccga ccgggtggtg tttgtcgacc acggccagat tattgaagaa
     1741 gccccgccgg gcgagttttt cacccggccg cagcacgagc gcgccaagca gtttttgaag
     1801 caggtgctct cgccgatgca ccagccagag taagcgcggc tcacaaaacc ctcctggcgg
     1861 tgttgcgcgg ccttgccgta ctgtttgtac tgtctacggc cgcccgccta gccaggaacg
     1921 ctgcgctgag ttttgtgaac cacgctaatc accgccactc ccgccgccga ttgccggcgg
     1981 cggccgttgc cgtatccgtc cagaagaagg agaacccgca tgcgcaccct cacccgcttt
     2041 gcccccctgg cgattgccgc cgccctctgt gctgcgccgg ccctggccga cgatctgccg
     2101 accctgcaaa agatcaagag cagcggcacg attgtcgtgg gccaccgcga atcgtcgatt
     2161 ccgttctcct acctggacgg caaccaaaag ccagtgggct attcgatgga tctgtgcaac
     2221 aagatcgtcg aagaagtgaa aaaagaactg aaaatgccgg cgctggtgac caagctcacc
     2281 ccggtgacct cgcaaacccg ca
//
LOCUS       DAOPYX010000386         2289 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_68511_length_2289_cov_4.014772, whole genome shotgun sequence.
ACCESSION   DAOPYX010000386 DAOPYX010000000
VERSION     DAOPYX010000386.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 2289)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2289)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2289
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68511_length_2289_cov_4.014772"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(68..322)
                     /locus_tag="PKN55_13685"
     CDS             complement(68..322)
                     /locus_tag="PKN55_13685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX72344.1"
                     /translation="MISSLRPLALILWLSVATLTGTRLWFVYPDFTPPFSPAFGVWLV
                     TVLHWPKGDVELALALCCSFVATLSLTLLAWALWRMWCRR"
     gene            complement(335..817)
                     /locus_tag="PKN55_13690"
     CDS             complement(335..817)
                     /locus_tag="PKN55_13690"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HNX72345.1"
                     /translation="MRFVLLIVLSALSLAVCAEPTVSVVRVLKSERKLQLWAGPQLLH
                     EFPVALGSSPLGHKQQEGDGKTPEGRYVLDYRKADSAFYKALHVSYPNAQDLAAARAR
                     GVNPGGLIMIHGQKNGLGWLAFLSQRFDWTQGCIALRNEHMDILWRLTPLGTPVDIRP
                     "
     gene            1278..2168
                     /locus_tag="PKN55_13695"
     CDS             1278..2168
                     /locus_tag="PKN55_13695"
                     /inference="COORDINATES: protein motif:HMM:NF024700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNX72346.1"
                     /translation="MTSEAVNLQDEQIFRVSGSRFKLLGQWHEITHDYTGSLLASLKD
                     SLLSPELKTLRDTIRNIRSLELLSPHLLRKRSRAQDKDIGAGGEKLSGFLATLKGEQK
                     AQLLAVLRQFYPQLRDFRVANVRGGWKRLMVTEAMQSAADPTQEVLLETEAGQLNDGL
                     LRILAMLAQANSQRSGMLLLDEVENGINPEIVEKLVNALLNSPVQVLVTSHSPMILNY
                     LDDDVARKAVQFVYKNPRGQTRVRPFFELPGIGDKLNYMGPGEAFVDTNLAALTDECV
                     ALDAAKDAEEAAKAADKARP"
BASE COUNT          525 a          645 c          709 g          410 t
ORIGIN      
        1 tcggccagac tcaggcatgt ggctggtgac tacgcgggga tgatagcgcg gattgggggc
       61 ggcatggcta acgccgacac cacatccgcc acagcgccca ggccagcagg gtgaggctca
      121 gtgtggccac gaacgaacag cataaggcca gcgccagctc gacatcgccc tttggccaat
      181 gcagcaccgt caccagccag acgccgaacg ctggcgaaaa cggcggggtg aaatccggat
      241 agacaaacca caggcgcgtg ccggtcaggg tggcgacgga tagccagagg atcagggcaa
      301 gggggcgaag ggatgagatc atgcgccagc ctgatcacgg ccggatatct accggcgtgc
      361 ccaatggcgt cagccgccat aaaatatcca tatgctcatt gcgcaaggca atacagcctt
      421 gcgtccagtc gaagcgctgg ctgagaaacg ccaaccagcc caggccgttt ttctggccat
      481 gaatcatgat caagccgcca ggattcaccc cgcgcgcccg cgcggcggcc aggtcttgcg
      541 cattggggta agaaacatgc agcgccttgt aaaacgcgct atcggctttg cgataatcca
      601 gcacatagcg cccctccggc gtcttgccgt cgccctcctg ctgtttgtgg cccagcggac
      661 tgctgcccag ggcaacagga aactcgtgca ataactgcgg cccggcccac aactgaagct
      721 tgcgctcgct tttcagcacc cgcaccacac tgaccgtggg ctcggcgcac acagccaggg
      781 acagcgcaga cagcacaatc aataggacaa aacgcatgga ggagggagcg ggcgtcaagt
      841 ggatgggcta tggtgctgcg cgggagggcg tgggtcaagt gggggtgaga tgcacatgcg
      901 ggtggcaagc tgggcgattt ggtggacaat gcagcagata tcgtgaggtg ggatgaataa
      961 tgattcaaat ggtgatcatt gcgaacttca aatcgctaga gaacatcacg cttctcttgg
     1021 acaaatttaa ttgcctggtc ggcatgaatg gcgcaggcaa aagcactgtc ttgcaggcct
     1081 tggatctctt ggcgcagttg atgcatggca atgtggatca gtggctaaag accagaggct
     1141 gggaaagtaa ggatttgccg tgccggacaa gtcatcctca cgcaacactc catagcctct
     1201 tattgggagt gacgatgcgc ctggaagacg gccgaggact tacttggata gctactctca
     1261 accgccatac attgcgcgtg acctcagagg cagtaaacct ccaagatgag caaattttta
     1321 gggtatcggg cagtcggttt aagctgcttg gccagtggca tgaaatcacc catgactaca
     1381 ccggctcact gctagccagc ctgaaagaca gcctactgtc gcccgagctg aaaaccctgc
     1441 gcgacaccat ccgcaatatt cgctcgctgg agctgctgtc gccgcatttg ctgcgcaaac
     1501 gctcgcgcgc gcaagacaag gacattggcg cgggcggaga aaaattgtcc ggctttctag
     1561 ctacactcaa gggcgaacaa aaagcccagc tgctggcggt gttgcgccag ttttaccccc
     1621 agctgcgcga ttttcgggtg gccaatgtcc ggggtggctg gaagcgcttg atggtcaccg
     1681 aggcgatgca gtcggcggcc gaccccacgc aggaagtgtt actggaaacc gaagctggtc
     1741 aactgaatga cggcttgttg cgcattctgg ccatgctggc gcaggccaac agccagcgca
     1801 gcggcatgct gctgctggat gaagtagaaa acggcatcaa cccggaaatt gtcgaaaagc
     1861 tggtcaatgc cctgctcaac agcccggtgc aggtgctggt cacctcgcac agcccgatga
     1921 ttttgaatta cttggacgac gacgtcgcac gcaaagccgt gcagtttgtc tacaaaaacc
     1981 cacgcggcca aacccgagtg cggccttttt ttgagctgcc gggcattggc gacaagctga
     2041 actatatggg cccaggtgag gcttttgtgg acaccaatct ggcggcgctg accgatgaat
     2101 gcgtggcgct ggacgccgcc aaggacgctg aagaggccgc caaggcggca gacaaggcgc
     2161 ggccatgatc gtggtgctga gtggagaagg ccgctccgac ctgggcgcgt gccgcaatgc
     2221 gcaaggccat tgctcaggcg acacattcgc gccaggcccg atgaccgtgc tggtggacca
     2281 ggaaatcgc
//
LOCUS       DAOPYX010000387         2282 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_68796_length_2282_cov_9.227211, whole genome shotgun sequence.
ACCESSION   DAOPYX010000387 DAOPYX010000000
VERSION     DAOPYX010000387.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 2282)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2282)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2282
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68796_length_2282_cov_9.227211"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(22..966)
                     /locus_tag="PKN55_13700"
     CDS             complement(22..966)
                     /locus_tag="PKN55_13700"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNX72347.1"
                     /translation="MRDLTPFSNRLSKNHRHLAKWAKRQDTECYRLYDRDIPEFPVIV
                     DVYGASAHIQEVDTGWQIDEDSHAAWLDAIRQAVAEVLAIAVDDIALKTRRRQKGLAQ
                     YEKTGETGRERIVSETGRRFIVNLEAYLDTGLFLDHRNTRRVVGEHAAGKRFLNLFAY
                     TGSFTVYAATGGAVSSVTVDLSNTYQDWSRRNFELNGLDLSRHSLVRADVFRYLQDAA
                     AAGERFDLIVMDPPSFSNSKKMLDILDVQRDQQRLVDGAMALLAPGGVLYFSNNLRGF
                     KLDPALAERYRVEDISAKSVPEDFRNRKIHQCFRVTAR"
     gene            complement(982..>2282)
                     /gene="wbaP"
                     /locus_tag="PKN55_13705"
     CDS             complement(982..>2282)
                     /gene="wbaP"
                     /locus_tag="PKN55_13705"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="undecaprenyl-phosphate galactose
                     phosphotransferase WbaP"
                     /protein_id="HNX72348.1"
                     /translation="RLTVWLMLLLAAISWFWLVRGHYTQRRPFWDETRDVVIAMLVLA
                     VVDAALMFMLKSPFSRLWWVATWAVSLFGVVLGRVLARRLMIWLGWWQRQVLVFGFGE
                     NAREAFAALKSERWLGYQVVGFVAPCADVRDAENLDDQPICGKPLLPYLLEEPAPRFL
                     ERFHQPAILIALETEQLDVQQQLVQRFSIAGADVSIAPPLKGLPLYGAELTHFFSHEV
                     LLLRVRNNLSRRASRFIKRTFDIVAASLLLLALAPLLLSVAWLVSRDGGPATFGHTRI
                     GRHGKPFRCLKFRSMVIDAQARLSELLARDPEARAEWERDFKLKNDPRITPLGNFIRK
                     TSLDELPQLINVLRGDMSLVGPRPVIADEVERYGEDKVFYLMARPGITGLWQISGRND
                     IDYDSRVALDCWYVRNWSLWYDIVILIKTIKVVLARDGAY"
BASE COUNT          416 a          710 c          749 g          407 t
ORIGIN      
        1 gctgggcgag tgcttgtcgc cttatcgcgc cgtcacccga aaacactgat gaatcttgcg
       61 gttgcgaaaa tcttccggca ccgatttggc gctgatgtct tccacccgat agcgctcggc
      121 cagtgccggg tcgagcttga agccgcgcag gttgttagag aaatacagca cgccgccggg
      181 ggcgagcagg gccatggcgc cgtccaccag gcgttgctgg tcgcgttgca cgtcgaggat
      241 gtcgagcatt tttttcgagt tggaaaaact cggcgggtcc atgacgatca ggtcaaagcg
      301 ctcgccagcc gcggcggcgt cttgcaggta gcggaacaca tcggcgcgca ccaggctgtg
      361 gcggctcagg tccaggccgt tgagctcgaa attgcgccgt gaccagtctt ggtaggtgtt
      421 ggacagatcc accgtcaccg acgagacggc cccgccggtg gcggcgtaca cggtgaagct
      481 gccggtgtag gcaaacagat tcagaaagcg tttgccggcg gcgtgttcgc ccaccacgcg
      541 gcgggtgttg cggtggtcca gaaacaggcc ggtgtccaga taagcttcca gattgacgat
      601 aaagcggcgg ccggtttcgc tgacgatgcg ctcgcggccg gtttcgccgg ttttttcgta
      661 ttgtgccagg cctttttgcc ggcggcgggt tttcagggca atgtcgtcca cggcgatggc
      721 cagcacctcg gccaccgctt ggcggatggc gtccagccag gcggcgtggc tgtcttcgtc
      781 gatttgccag ccggtgtcca cttcttgaat gtgggcgctg gcgccgtaga catcgacaat
      841 caccgggaac tccgggatgt cgcggtcgta cagccggtag cactcggtgt cctggcgctt
      901 ggcccatttg gccaggtggc ggtggttttt gctcagccga ttgctgaagg gggtgaggtc
      961 gcgcatgacg ggctccgtgg gtcagtaggc gccgtcgcgc gccagcacca ctttgatggt
     1021 tttgatcaaa atcacgatgt cgtaccacag cgaccagttg cgcacatacc agcagtccag
     1081 cgccacgcgg ctgtcgtagt cgatatcgtt gcggccgctg atttgccaca ggccggtgat
     1141 gccggggcgc gccatcaggt aaaacacctt gtcttcgccg tagcgctcca cctcgtcggc
     1201 aatcaccggg cgagggccaa ccaggctcat gtcgccgcgc agcacattga tcagctgcgg
     1261 cagctcgtcc aggctggtct tgcggatgaa attgcccagc ggggtgatgc gcgggtcgtt
     1321 tttcagcttg aaatcacgtt cccactcggc gcgggcttcc gggtcgcgcg ccaacagctc
     1381 gctcagccgc gcctgggcgt cgatcaccat cgagcggaat ttcaggcagc gaaacggttt
     1441 gccgtggcgg ccgatgcggg tgtggccaaa ggtggccggg ccgccgtcgc gcgacaccag
     1501 ccaggccacg ctcagcagca gcggggccag cgccagcagc agcaggctgg cggcgacaat
     1561 gtcaaaagtg cgcttgataa agcgcgaggc gcggcgcgac agattattgc gcacgcgcag
     1621 cagcagcact tcgtggctga aaaaatgggt cagctcggcg ccatacagcg gcaggccttt
     1681 cagcggcggg gcaatgctga cgtcggcgcc agcaatgctg aagcgctgca ccagctgttg
     1741 ttgcacgtcc agttgttcgg tttccagcgc aatcagaatg gccggctggt gaaaacgttc
     1801 cagaaagcgc ggggcgggtt cttccagcag atagggcagc agcggtttgc cgcagatcgg
     1861 ttggtcgtcg aggttttccg cgtcgcgcac atcggcgcac ggggcaacaa agcccaccac
     1921 ctgatagccc agccagcgct cgcttttcag cgcggcaaac gcttcgcggg cgttttcgcc
     1981 aaagccgaac accagcacct ggcgctgcca ccagcccagc cagatcatca gccgtcgggc
     2041 cagcacccgg cccagcacca cgccaaacag cgacaccgcc caggtggcca cccaccacag
     2101 ccgcgagaac ggcgacttga gcatgaacat cagcgcggcg tccaccaccg ccagcaccag
     2161 catggcgatg accacatcgc gggtttcgtc ccagaacggc cggcgctggg tgtagtggcc
     2221 gcgcaccagc cagaaccagc tgatggcagc cagcagcagc atcagccaga cggtcagccg
     2281 ca
//
LOCUS       DAOPYX010000388         2275 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_69148_length_2275_cov_12.801351, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000388 DAOPYX010000000
VERSION     DAOPYX010000388.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 2275)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2275)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2275
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69148_length_2275_cov_12.801351"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..937)
                     /locus_tag="PKN55_13710"
     CDS             complement(<1..937)
                     /locus_tag="PKN55_13710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012697279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HNX72349.1"
                     /translation="MTSIVFLDRDSLPTAVPPLPLPHRWQDYPATSPEQLLSHASGAQ
                     VLISNKVPLRAEHFAQLPELKLVAVAATGVNQIDLDAARAHGVAVCNVRNYGADAVAE
                     HALMLMLALSRNLFAYRRAVLEGGWQQSAQFCLHAAPMKDLSARTLAVCGGGAIGEAL
                     AAKAAALGMRLMRVERKGAAQVRAGYTRFETALAEADVLSLHLPLNNDTRGLIGAAEL
                     ALMKPDALLINTARGGLVDEAALLAALQAGRLGGAGLDVLDTEPPRQGNPLLDVELPN
                     LIITPHAAWLSEQALAKLAQQLVDNIAAFLQGEPRN"
     gene            1122..1451
                     /locus_tag="PKN55_13715"
     CDS             1122..1451
                     /locus_tag="PKN55_13715"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008952500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HNX72350.1"
                     /translation="MSTSLLIEDLTVGDGAEAVTGKEVTVHYTGWLTDGQKFDSSKDR
                     MQPFSFPLGAGHVIKGWDQGVAGMKVGGKRKLTIPPELGYGARGAGGVIPPNATLVFE
                     VELLSID"
     gene            1545..2162
                     /locus_tag="PKN55_13720"
     CDS             1545..2162
                     /locus_tag="PKN55_13720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008952192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HNX72351.1"
                     /translation="MLTLYTWGTPNGRKVSILLAELGLEHHVQPINILTGEQNTPAFR
                     AINPNGKIPALVDSDGPDGQPISVFESAAIMIYLAEKHQRFLPASGAERYAVLQWLMF
                     QMGGFGPMLGQAHHFLRFAPEPVPYAIERYRNEARRLYTVLDNQLAGRDYVAGDYSIA
                     DMALYPWATRHEWQEIDLADFPNVARWMTTLAARPAVAKGMAEPG"
BASE COUNT          418 a          812 c          682 g          363 t
ORIGIN      
        1 gattgcgcgg ctcgccctgc aaaaacgcgg cgatattgtc caccagctgc tgcgccagct
       61 tggccagcgc ctgctcgctc agccaggcgg cgtgcggggt gatgatcaga ttgggcagct
      121 cgacatccag cagcgggttg ccctggcgcg gtggctcggt gtccagcaca tccagcccgg
      181 cgccgcccag ccggccggcc tgcaaggcgg ccagcaacgc cgcctcgtcc accagcccgc
      241 cgcgcgcggt gttgatcagc agcgcgtccg gcttcatcaa ggccagctcg gccgcgccaa
      301 tcaggccacg ggtgtcgttg ttcagcggca gatgcaagct cagcacatcc gcctcggcca
      361 gcgccgtctc aaaccgggta tagcccgcgc gaacctgcgc cgcgcccttg cgctccacgc
      421 gcatcaaccg catgcccagc gccgccgcct tggcggccag cgcctcgcca atcgccccgc
      481 cgccgcacac cgccagcgtg cgcgccgaca ggtctttcat cggcgcggcg tgcaggcaaa
      541 actgcgccga ctgctgccag ccgccttcca gcacggcgcg ccgataggca aacagattgc
      601 gcgacagcgc cagcatcagc atcaaggcgt gctcggccac cgcatcggcg ccgtagttgc
      661 gcacattgca caccgccacg ccatgcgcgc gcgccgcgtc cagatcaatc tggttcaccc
      721 cggtggccgc caccgccacc agcttgagct cgggcagctg ggcaaaatgc tcggcgcgca
      781 gcggcacttt attgctgatc aacacctgcg cgccgctggc atggctcagc agctgctcgg
      841 gcgaggtggc cgggtaatct tgccagcggt gcggcaatgg cagcggcggg acggcggtgg
      901 gcaggctgtc gcgatccaga aacacaatcg aggtcatggc gggggctttc cagagtcaaa
      961 aggtcagacc cgcagcatgc ctgaattcca cccacaggaa aatcctgccc catcgcgggc
     1021 gcaaaaggcc atccgtgggg ttctacgccg tcgtttcgcc gcttacaatg ctcacagcct
     1081 ggcccatccg gccagacatt cgcattcacc aaggaaccct catgagcaca tcgctgttga
     1141 ttgaagacct caccgtgggc gacggcgccg aagctgtcac cggcaaggaa gtcaccgttc
     1201 actacaccgg ctggctgacc gacggccaga aatttgactc cagcaaagac cgcatgcagc
     1261 cgttcagctt tccgctgggc gccggccatg tgatcaaggg ctgggatcaa ggtgtggccg
     1321 gcatgaaagt gggcggcaag cgcaagctga ccatcccgcc ggagctgggc tacggcgcgc
     1381 gtggcgctgg cggcgtgatt ccgcccaacg ccaccctggt gtttgaagtg gaactgctgt
     1441 cgatcgactg acgcccacgg gcaacgggcg cgccctgcgt ccgttgccgc cccgccagcg
     1501 ccggtatacc ctcgggtttc ccttcaccgg acacaggcgc acacatgctc accctctata
     1561 cctggggcac ccccaacggc cgtaaagtct ctatcctgct ggccgaactc ggcctggaac
     1621 accacgtcca gccgatcaat atcctgaccg gcgaacaaaa caccccggcg tttcgcgcca
     1681 tcaaccccaa tggcaaaatt cccgcgctgg tagacagcga cggccccgat ggccagccga
     1741 tcagcgtgtt tgaatccgcc gccatcatga tttacctggc agaaaaacat cagcgcttct
     1801 tgcccgccag cggcgccgaa cgctatgccg tgctgcaatg gctgatgttc cagatgggcg
     1861 gctttggccc catgcttggc caggcgcacc actttttacg ctttgccccc gaacccgtcc
     1921 cctacgccat cgagcgctac cgcaacgaag cccgccgcct gtacaccgtg ctggacaatc
     1981 aactggccgg gcgcgactac gtggccggtg attattcgat tgccgatatg gcgctctacc
     2041 cctgggccac ccgccacgaa tggcaggaga ttgatctggc ggacttcccg aatgtcgccc
     2101 gctggatgac cacgctggcg gcacggccgg cggtggcgaa ggggatggca gaaccggggt
     2161 aactccccac tgcgccgcac cctggccacc ttcaccctca tgggtggcca ggacgccagc
     2221 aagcatcatg gatgaaatgc tggcgagaat ggcgccgatt ctcgccccac ctaag
//
LOCUS       DAOPYX010000389         2256 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_70067_length_2256_cov_10.000000, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000389 DAOPYX010000000
VERSION     DAOPYX010000389.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 2256)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2256)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2256
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70067_length_2256_cov_10.000000"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(194..1063)
                     /locus_tag="PKN55_13725"
     CDS             complement(194..1063)
                     /locus_tag="PKN55_13725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012697956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HNX72352.1"
                     /translation="MKVGYIGLGIMGRACAANLLRAGHTLSVYARRPESAAELVAQGA
                     RVYASPAELAREVEIVISNVSDTPDVEEVMLGEHGVIHGAHPGLIAIDMSTIKPLAAR
                     DIAAKLAAAGVDFLDAPVSGGEIGAINGTLTIMVGGKAEALDTARPVLEAMGKTITRI
                     GDSGAGQVAKACNQIVVAVTIEAVAEAMALARACGVDPAPVREALLGGFAGSRVLDVH
                     GQRIIDDNYAPGFKAKLHQKDMGIVADTAAALGIRLDAASHAAELIQQLVAQGDGELD
                     SSAIARLIASRRA"
     gene            1289..>2256
                     /locus_tag="PKN55_13730"
     CDS             1289..>2256
                     /locus_tag="PKN55_13730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011137484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase/aspartoacylase
                     family protein"
                     /protein_id="HNX72353.1"
                     /translation="MTVPMHFQSVHFSAIEPGPRLIVLGAVHGNEVCGRLGILRVIDD
                     LNSGRTRLLRGSVTFVPVTNPLAYNKVQRAGDRNLNRNLAPTDAPQEFEDHIANWLCP
                     QLAAHDVLLDLHSFLAPGVPFAFMGPRNNDGPLEPFAFAEQEQALAVRLGVNRFIEGW
                     LSTYALGVERRVKVFGHQTDRATQLNTDTRYGVGTTEYMRAHGGWALTLECGQHADPQ
                     APDVAYTAICNTLAHLGLSDAPAPEAQPASDCEMLALYEVIDKMHADDRFVRPFASFD
                     AVKQGEQIGVRHDGAPVLAAEDGCIVFPNPNAQVGQEWFYLARPSNR"
BASE COUNT          365 a          735 c          722 g          434 t
ORIGIN      
        1 ctagccagga tcgcttcgct gggttttgtt agccgctcta aacacctgtt gaagcccgtt
       61 ctgttcgcgc agcttgtcat gcagtgtgtt gatttttcag atttttactg tttgatggac
      121 tatgtttcga atacagcggc atcaggctga ggcacccacc ccatgctggc ttcgggtggg
      181 cgcctgttca cgctcaggcg cgtcggctgg caatcagccg ggcaattgcc gaagaatcca
      241 gctcgccatc gccttgggcc accaactgct gaatcaactc ggcggcgtgg ctggcggcat
      301 ccaggcggat gcccagcgcg gcggcggtgt cggcgacaat ccccatgtct ttttggtgca
      361 gcttggcctt gaagccaggg gcgtagttgt cgtcgatgat gcgttggccg tgcacatcca
      421 gcacgcggct gccggcaaag ccgcccagca gcgcctcgcg caccggagcc ggatccaccc
      481 cgcaggcgcg cgccagcgcc atcgcctcgg ctactgcctc gatggtgacc gccaccacaa
      541 tctgattgca ggccttggcc acctggccgg cgccggagtc cccaatgcgg gtgatggtct
      601 tgcccatggc ttccagcacc gggcgggcgg tatccagcgc ctcggccttg ccgcccacca
      661 tgatggtcag cgtgccgttg atggcgccaa tctcgccacc cgacaccggc gcatcaagaa
      721 aatccacccc ggcggcggcc agtttggcgg cgatgtcgcg cgcggccagc ggcttgatgg
      781 tgctcatgtc gatggcaatc agccctgggt gggcgccgtg gatcacgccg tgttcaccca
      841 gcatgacttc ttccacgtcc ggggtatccg agacattgct gatgacaatc tccacctcgc
      901 gcgccagctc ggccgggctg gcatacaccc gcgcaccctg ggcgaccagt tcggcggcac
      961 tttccgggcg acgggcgtaa accgatagcg tatggccggc gcgcagcaga ttggccgcgc
     1021 aagcgcgccc catgatgccc aggccgatgt aaccgacttt catgtgaatt cctttatgct
     1081 gagtgtgcca ggcgggccgc tggcggccgc gctgcttgag gctgacaatc gtagcagaca
     1141 atgccggcgt gagtggggag atacccgcct cctgcgcctg gcggcattgc cgcaccgaca
     1201 ccttaatccg ccacgactgg tttgcatccc acgctgacgc gcggcacaat ccgcgcattt
     1261 cccattctct gctctgccga ggcgcgtcat gaccgttccc atgcatttcc agtctgttca
     1321 cttttccgcc atcgaacccg gcccccggct gattgtgctg ggcgcggtgc acggcaacga
     1381 ggtgtgcggc cggctgggca ttttgcgggt gatcgacgac ctcaacagcg gtcgcacccg
     1441 tttgctgcgc ggcagtgtca cctttgtgcc ggtcaccaat ccgctggctt acaacaaggt
     1501 gcagcgcgcc ggcgaccgca acctgaaccg caatctggcg ccgaccgatg cgccgcaaga
     1561 gtttgaagac cacatcgcca actggctgtg cccgcagctg gctgcgcatg atgtgctgct
     1621 ggatttgcat tcgtttctgg cgccgggcgt gccgtttgcc tttatggggc cgcgcaataa
     1681 cgacggcccg ctggagccgt ttgcctttgc cgagcaagag caggcgctgg cggtgcggct
     1741 gggggtgaac cgctttatcg aaggctggct gtccacctat gcgctgggcg tcgagcggcg
     1801 ggtgaaggtg tttggccacc agaccgaccg cgccacccaa ctgaacaccg acacccgcta
     1861 cggcgtgggc accaccgaat acatgcgtgc gcatggcggc tgggcgttga cgctggagtg
     1921 cggccagcac gccgacccac aggcgccgga tgtggcctac accgccattt gcaacaccct
     1981 ggcgcatctg ggtttgagcg acgcgccggc ccccgaggcg cagccggcca gtgactgcga
     2041 gatgctggcg ctgtatgaag tgatcgacaa aatgcacgcc gacgaccgct ttgtgcggcc
     2101 gttcgccagt tttgacgcgg tcaagcaggg tgagcagatt ggcgtgcgcc acgatggtgc
     2161 gccggtgctg gcggcggaag acggctgcat tgtcttcccc aacccgaatg cccaggtggg
     2221 gcaggagtgg ttttacttgg cgcgcccgag caatcg
//
LOCUS       DAOPYX010000390         2251 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_70336_length_2251_cov_6.587887, whole genome shotgun sequence.
ACCESSION   DAOPYX010000390 DAOPYX010000000
VERSION     DAOPYX010000390.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 2251)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2251)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2251
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70336_length_2251_cov_6.587887"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            122..1930
                     /locus_tag="PKN55_13735"
     CDS             122..1930
                     /locus_tag="PKN55_13735"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HNX72354.1"
                     /translation="MTYRKDIQILRGIAVLLVVLFHLEMAGFASGFLGVDVFFVISGY
                     LMAMMYQPQHKVAFFLKRAKRLLPAYFVTIGATVFGATLLVTPNDYQQVVNQSWFAVF
                     FASNIGFWSENSYFDKAAFKPLLHLWSLGVEIQFYLWVPVFYWLFKKHVGFFVLALLG
                     SLAACFFLLGMSPKTSFFWMPMRLWQFLLGVGCCYYLQHRPAPGSPWWGAAALLAIVA
                     IPFAPLNGEAMNAWHGHPGVNALAISLATSATLLYGLPKIVQSHAVATLLERLGGYSY
                     SIYLAHFPVIVLFLYQPFHGTVTHSATLQDTGLLIALIVAMSFLLYCCVERPFRHTER
                     IAYYWPAAVGVILLGGALGSQLQTRLIPSNEMKIYQAWFDRDVYRCGKINRLLDPLAK
                     SCEITENIQSARHRVLLVGNSHADSIKATFSQVAAAQHTAVYFMVENNPMMKGGMSPQ
                     RIMQEARLRQADTIVLHYSPEGLDFAAVSQLLALNQAPSLKLAFLMPVPVWRNHVPMT
                     LLAHEKTGKPLEQQALRDYLAANQPLSNYLSQLPSGALQVYATAPSLCQPVCAMQTAG
                     GQPLYFDSGHLTLTGSALLAPVFQRMLAELDAGRQK"
BASE COUNT          486 a          589 c          567 g          609 t
ORIGIN      
        1 ataacggccc cctcctaagg ggcagttaca ggttcgattc ctgtcggggg cgccaccggc
       61 atctcttcct catgcccaga aaaatccata tcaaaccgga tcgcatctga aagcatgaat
      121 catgacctat agaaaagaca ttcagatttt acgtgggatt gctgttttac tggtcgtgtt
      181 atttcatttg gagatggcgg gctttgccag cggtttttta ggggtagatg tattttttgt
      241 catcagtggc tatctgatgg ccatgatgta tcaacctcag cataaagtgg cattttttct
      301 gaagcgggca aagcgattgc ttcctgctta ttttgtcacc attggcgcca ccgtgttcgg
      361 cgccacctta ctggtgaccc ccaatgacta ccaacaagtc gtcaaccaat cttggtttgc
      421 cgtatttttt gcctccaaca ttgggttctg gtccgaaaac tcatactttg acaaagctgc
      481 attcaaacca ttattacacc tgtggtcttt aggggtagaa attcagtttt atttatgggt
      541 gcctgtattt tactggctat tcaaaaaaca cgtagggttt tttgttctgg cattattagg
      601 ctcactggcg gcgtgtttct ttttactcgg catgtcgcct aaaaccagtt ttttctggat
      661 gccgatgcgc ttatggcagt ttttgctggg ggtgggctgt tgttattatt tgcagcaccg
      721 tcctgcgcca ggctcaccct ggtggggcgc ggctgcatta ctggctattg tggcgattcc
      781 gtttgcgccg ctcaatggcg aagccatgaa cgcctggcat gggcatcctg gcgtgaatgc
      841 actggccatt agcttggcca ccagcgcaac attactgtat ggcttgccaa aaatcgtgca
      901 aagccacgcg gtggcgaccc tgctggagcg gctgggggga tattcttact cgatctatct
      961 ggcgcatttt cctgtcattg tattgttttt gtatcaacct ttccatggca cagtcacaca
     1021 cagcgccacg ttgcaagaca caggcttgct gattgcgctg attgtggcaa tgtcctttct
     1081 gctttactgt tgcgtggaaa gaccattcag acatactgaa cggatcgcat attattggcc
     1141 tgctgcagta ggggtgattc tgcttggcgg cgcattaggc tcgcagctac aaactcgatt
     1201 gattccatca aatgaaatga agatctatca agcctggttt gatcgtgacg tctatcgatg
     1261 cggaaagatc aatcgcctgc ttgatccctt ggctaaatca tgcgaaatca cggaaaatat
     1321 tcagtctgct cgccatcggg tgctcctggt gggcaacagt catgcggatt caattaaagc
     1381 cacattcagc caagtggctg ccgcacaaca caccgccgtt tattttatgg tggaaaacaa
     1441 ccccatgatg aagggcggca tgagtccgca gcgcattatg caagaagctc gtctgcgtca
     1501 ggcagacacc attgtcttgc attactctcc agaaggtttg gattttgccg ctgtcagtca
     1561 gctgcttgcc ttgaatcaag cgccatcgct gaagttagcg tttttgatgc ctgtgcctgt
     1621 gtggcggaat catgttccca tgacgctgct ggctcatgaa aaaacgggca agccacttga
     1681 gcaacaagcc ctgcgcgatt acctggccgc caaccagcca ctctcaaact atctgagcca
     1741 attgccatct ggcgcattgc aggtgtatgc cacagccccc agcttgtgcc aacctgtttg
     1801 cgccatgcag accgcaggcg gccaacccct gtattttgat agcggacact tgaccctgac
     1861 tggctctgcg ctgcttgcgc cagtgtttca acgcatgctg gcggagcttg atgccgggcg
     1921 acaaaaataa tcatgactta tgaatgacag cgtcggctca gcattgtttt tgtggcaagc
     1981 aagacgacgc gccattcagc accagcaaga ggacaactca gaacaccgag cacgggcgct
     2041 gcggctgcgg tgagtccgtt gtgtgaagaa cggctcacaa aggcctcggc tgggccaaag
     2101 tcactgaatg gcttgctctc gctgttgcgc cctgcattgc gccatcattg tccgccgtgt
     2161 ttttttgcgt ccggcaatca gatggtttca cgctccccca acaaccgccg atacagcccc
     2221 agcaactctt ccgccatcgc ttccagcgtg t
//
LOCUS       DAOPYX010000391         2248 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_70476_length_2248_cov_13.165983, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000391 DAOPYX010000000
VERSION     DAOPYX010000391.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 2248)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2248)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2248
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70476_length_2248_cov_13.165983"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            179..892
                     /locus_tag="PKN55_13740"
     CDS             179..892
                     /locus_tag="PKN55_13740"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNX72355.1"
                     /translation="MTDAHIVLTHGHLDTLETLSQALHHQGLTVATHSSWTALQAWLT
                     VRPASVLAIDLDLPDGQGLEMVRELRARYPQLGIIALSEDGSLNARIDGLSSGADAYL
                     VQPVDARELHLTVRTLLRRLPLAVGECPPGWALNPRTLTLTAPNGAHIALTHNETRVL
                     NAAARAEGALVSRRCLIEALGANYWDYDERRLEALISRLRRKLPGLSVRGIKGQGYVF
                     DSVRLDGPQPVSRVAEHVN"
     gene            complement(1022..2083)
                     /gene="tal"
                     /locus_tag="PKN55_13745"
     CDS             complement(1022..2083)
                     /gene="tal"
                     /locus_tag="PKN55_13745"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008952088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HNX72356.1"
                     /translation="MNRLQAIRPFGQRIWLDNLSRDLLRSGELSRWIDEDGIAGVTSN
                     PAIFHKAIRDDVRYVSALQELKALPDLDAEQRYEQLVIPDIQAACDLLEPQYRASQGD
                     DGYVSLEVSPALAHDAEGTVDAARRLWTAIDRPNAMIKIPATAAGVTALATLIREGIN
                     VNVTLLFSRAQVEATWAAYEAGLAARLADGQALGRVRSVASFFLSRIDSLLDPQLPEA
                     LRGKAAVALARDVYAAWQARLDSPLWARLRQAGAQPQWLLWASTGTKNPAYSDVLYVE
                     SLIGEQTVNTVPDATLLAFRDHGEAAATLAADPAGARAVLAAVAAAGVDLEAVGERLQ
                     VDGLKLFDDAFAALLALTA"
BASE COUNT          435 a          754 c          706 g          353 t
ORIGIN      
        1 taaatacatt gacacgaccc gaagcgtcca tcaggctttg tcaggatgga cgccttcgtc
       61 catcctgacg tcacaccccg gcaacaatca gaataaaaac aataaaatca agccactaaa
      121 tagctggcac aacgcttgca aatgtctggc atcccccaat aagagcaagg agacagccat
      181 gaccgacgcg cacattgtgc tgacccacgg gcatctcgat accctggaaa ccctgagcca
      241 ggccctgcat caccagggcc ttaccgttgc cacccacagc agttggactg ccctgcaagc
      301 ctggctgacc gtccgcccgg ccagcgtgct ggccattgat ctggatttgc ccgacggcca
      361 gggtctggag atggtgcgcg agctgcgcgc ccgctatccg cagctgggca tcatcgccct
      421 gtctgaagac ggcagcctga acgcccgcat cgacggcctg agcagcggcg ccgatgccta
      481 tctggtgcag ccggtggacg cgcgcgaact gcatctgacc gtgcgcacct tgctgcgccg
      541 cctgccgctc gccgtcggcg aatgcccgcc cggctgggca ctgaacccgc gcacgctgac
      601 gctgaccgcg cccaatggcg cgcatatcgc gctgacccac aacgaaaccc gcgtgctgaa
      661 cgccgccgcc cgcgccgaag gcgcgctggt cagccgccgc tgcctgattg aagcgctggg
      721 tgccaactac tgggattacg acgagcgccg gctggaagcg ctgatcagcc gcctgcgccg
      781 caaactgccg ggcctgagcg tgcgcggcat caagggccag ggttatgtgt tcgattcggt
      841 gcggctggat ggcccgcagc cggtcagccg ggtggccgag catgtgaact gagttcatct
      901 cgccaaacgg gattcagtca atctcgctgg ctttgtcggg taaacgacgc taaaaatacc
      961 cgcgccccac cgatgaagat caatcatcgg tggggcgcag tttttcagca ggcgaagcgg
     1021 attacgccgt cagcgccagc agcgcggcaa aggcgtcatc aaacagcttg agaccatcca
     1081 cttgcaggcg ctcgcccacg gcttccagat ccaccccggc ggcggccacg gcggccagca
     1141 cggcgcgcgc gccagccggg tcggcggcca gcgtggcggc ggcttcgcca tggtcgcgga
     1201 aggccagcaa ggtggcgtcc ggcacggtgt tcaccgtctg ctcgccaatc aggctctcga
     1261 catacagcac atctgaataa gccgggtttt tggtgccggt gctggcccac agcagccact
     1321 gcggttgtgc gccagcctgg cgcaaacgcg cccacagcgg gctgtccagc cgcgcctgcc
     1381 aagcggcgta gacatcgcgc gccagcgcca ccgccgcctt gccgcgcaag gcctcgggca
     1441 gttgcggatc cagcaggctg tcgatgcgcg acaggaagaa actggccacc gagcgcaccc
     1501 ggcccagcgc ctggccatcg gccagacgcg ccgccagccc ggcctcgtac gccgcccagg
     1561 tggcttccac ttgcgcgcgc gagaacaaca gcgtgacgtt gacgttgatg ccctcgcgga
     1621 tcagcgtggc cagcgcggtg acgccagcgg cggtggccgg aatcttgatc atggcgttgg
     1681 gccggtcaat cgccgtccac agccggcggg cggcgtccac cgtgccctcg gcgtcgtgcg
     1741 ccagcgccgg cgagacttcc aggctgacat agccatcgtc gccctgactg gcgcgatact
     1801 gcggctccag cagatcgcag gcggcctgaa tatcaggaat caccaattgt tcgtaacgct
     1861 gctcggcgtc caggtcgggc agcgccttga gttcttgcag ggcgctgaca tagcggacat
     1921 catcgcggat ggctttgtga aaaatcgccg ggttggaagt caccccggca atgccgtcct
     1981 cgtcgatcca gcgggacaac tcaccgctgc gcaagaggtc gcgggacagg ttgtccagcc
     2041 agatgcgctg gccgaacgga cggatggctt gcaatcgatt catacgggtc tttctggcgg
     2101 tgcgaagagg gcgggcgctg gctgcccgca ggcagcaagc cccccattat gccagcggca
     2161 cggcgaacgg gaagcaggaa atgcgccaaa gattgaccag cgccaagcca gagacagtca
     2221 agcgcaaaac agacttgttt taaaacat
//
LOCUS       DAOPYX010000392         2242 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_70775_length_2242_cov_7.386831, whole genome shotgun sequence.
ACCESSION   DAOPYX010000392 DAOPYX010000000
VERSION     DAOPYX010000392.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 2242)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2242)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2242
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70775_length_2242_cov_7.386831"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            211..924
                     /locus_tag="PKN55_13750"
     CDS             211..924
                     /locus_tag="PKN55_13750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX72357.1"
                     /translation="MLLELELVLLVVCSLVAPAGAYAFLYGRWSISRRTVLGFAMLLI
                     VLAGVDVALLQSLTEKARRTPDLPGMQLFTDQLALVLYVLPAALAGLGVNLLSHVLIS
                     HLGQAERRFDHQHSHATSTPHLQHWQTGLVMLAIGAIFLLDLYSGNDIRLHALYLFPL
                     AWVARHRQGWLPTTLTLLTVSASQIVTFSLAAAGMASFITDALVSLSASCLVVVLARQ
                     SGQRQQERPFAPRQARDIR"
     gene            complement(1217..1528)
                     /locus_tag="PKN55_13755"
     CDS             complement(1217..1528)
                     /locus_tag="PKN55_13755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX72358.1"
                     /translation="MNAAKKIRKLIESGAAEGDVAVLVDLARSLETGVPFQLSQLNAL
                     DMRNFDLAMELIADWRFDHHIPARRKLTDVIMNLHEESAAESVDLAPVAERIEAADKV
                     E"
     gene            1852..2055
                     /locus_tag="PKN55_13760"
     CDS             1852..2055
                     /locus_tag="PKN55_13760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008953628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2788 domain-containing protein"
                     /protein_id="HNX72359.1"
                     /translation="MSEEQFTNISMAVLLTGLILFMGFIIWDLGKKSNAGRFGMFVLF
                     LALGFGVTGFVFKELLVSYIIAK"
     gene            2089..>2242
                     /locus_tag="PKN55_13765"
     CDS             2089..>2242
                     /locus_tag="PKN55_13765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014087393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF520 family protein"
                     /protein_id="HNX72360.1"
                     /translation="MPSFDIVSEVDVVEIKNAVEQCNKEVSNRFDFKGSDARVEQGDK
                     VLTVFAD"
BASE COUNT          410 a          661 c          664 g          507 t
ORIGIN      
        1 cagcaacaag ccaaagaagg gaccgtcgat tctgccagac cgaatccagt gcccgcttgc
       61 gcatgacaca cactgttgca ctttgttacc gctggcggtg cgccatcgcg ctatgctgcg
      121 acattgccgc cagaatccca tggaactcga tatgcccctc cgcagcccca tccagccatc
      181 ccatccgggc atccgggcat gaaatcaggc gtgctgctgg aactggagct ggtgctactg
      241 gtggtgtgtt cgctggtggc gcccgccggg gcttatgctt ttctgtatgg tcgctggtcg
      301 atttcgcgac ggaccgtgct gggctttgcc atgctgctga ttgtgctggc cggggtggat
      361 gtggcgctgc tgcaatcgct gaccgaaaaa gcccggcgca cgccagacct gcctggcatg
      421 cagttgttta ccgatcagct ggccctggtg ttgtatgtgc tgccggcagc cctggccggc
      481 ttgggggtga atctgctttc gcatgtgctg atcagccatc tgggacaggc cgaacggcgg
      541 tttgaccatc agcacagcca tgccaccagc acgcctcatc tgcagcactg gcagacgggg
      601 cttgtcatgc tggcgattgg cgctattttt ttgctggatc tttacagtgg caacgatatc
      661 cggctgcacg cgctgtatct gtttccactg gcctgggtgg cccggcatcg ccaaggctgg
      721 ctgcccacta cgctgacctt gctgacggtc agtgcctcgc agatcgtgac tttttcgctg
      781 gccgccgccg gcatggccag ctttatcacc gatgcgctgg tgtcgctgtc ggcctcgtgc
      841 ctggtggtgg tattggcccg gcagtctggc caacgccagc aggaacgtcc gtttgcgcca
      901 cggcaggcgc gggatatccg ttgagcgctg tgcgggctgc gtcgcgctgg caccgatagt
      961 acgctggtaa tgcgctggcg aaaggcacgg ctcagctgtg ccgattggcc aggcgtcagg
     1021 ccggcagcca aagtgatttg gcgacgtatg aacggcgtgc cagccgggtc tgtgcaggcg
     1081 gccaaagcgc acaggcatat aatggatgat attgcacttt atggaaaaat aagtgccatg
     1141 tgttgcactt aatcgcctgt aaaaacaaca gccgccgacc aaaggtcggc ggctgtgcat
     1201 gcaagcgcgc agtggctcac tccaccttgt cggcggcctc aatacgctcg gccaccgggg
     1261 ccagatccac cgactcggcg gcgctttcct cgtgcagatt catgatgaca tcggtcagtt
     1321 tgcgccgtgc cgggatgtga tggtcaaagc gccaatcggc aatcagctcc atggccagat
     1381 cgaaattgcg catgtccagc gcgttcagtt gcgacaactg aaaaggcaca ccggtttcca
     1441 ggctgcgcgc cagatccacc aacacggcca catcgccctc agcggcgccg gattcgatca
     1501 atttgcggat tttttttgct gcattcatgt cacgttcctg gtgagtgtcg aacctgtcgt
     1561 gatgatacgg ggggtttgca ctgcacaata ttaagcttgt gttgccatta tatgacatgc
     1621 cggccgcttt gcccggcact ttgtgcgccg tatccgggcc ggcgcccggg gccagatcag
     1681 cacatgcagc cacctggcga acctcacccg gaccctgaac ccgggcaaaa gttccttggc
     1741 cgtccgtcac caggccacgt cagtggcagg tgtcgtttcc ccatgctgtc cttgtctata
     1801 caatatcgct tttttcatcc tcggcgcgcg ccgatgctgg agcagaaccg catgtctgaa
     1861 gaacaattca ccaatatctc gatggccgtg ctgcttaccg gtctgatcct gttcatgggc
     1921 ttcatcatct gggatctggg caaaaagtcc aacgccggcc gctttggcat gttcgtgctg
     1981 tttctggcgc tgggttttgg cgtcaccggc tttgtcttca aggaactgct ggtcagctac
     2041 atcatcgcca aataagcgca gccgggttta cccgcctcag gaaatgttat gccgtcattt
     2101 gatattgttt cagaagtcga tgtcgtcgaa atcaagaacg ccgtcgagca gtgcaacaaa
     2161 gaagtcagca accgctttga cttcaagggc tcggacgccc gcgtcgagca gggcgacaag
     2221 gtgctgaccg tgtttgccga cg
//
LOCUS       DAOPYX010000393         2229 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_71380_length_2229_cov_9.776449, whole genome shotgun sequence.
ACCESSION   DAOPYX010000393 DAOPYX010000000
VERSION     DAOPYX010000393.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 2229)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2229)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2229
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71380_length_2229_cov_9.776449"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..251)
                     /locus_tag="PKN55_13770"
     CDS             complement(<1..251)
                     /locus_tag="PKN55_13770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX72361.1"
                     /translation="MGLFDMFKSDSGEKMTPHLAFATSLLYMMSADGEMDNEEIGHLL
                     SVLGGQDDGRGTIGVGAQNQALLDSALKYRRKNSVETFL"
     gene            complement(622..2133)
                     /locus_tag="PKN55_13775"
     CDS             complement(622..2133)
                     /locus_tag="PKN55_13775"
                     /inference="COORDINATES: protein motif:HMM:NF015863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY-associated TM helix domain-containing
                     protein"
                     /protein_id="HNX72362.1"
                     /translation="MKRTLFILHRWLGIALGLFMLIWFFSGLVMIYAGASRVDSLTRL
                     AHSQALPESAPPLSAGQAWRRAWPERADEAPAEARLMMQAGEPMWLLIDLAGQRHMLS
                     AVDGRRVSTDAARAVRLAQDWARAAGRAATPSFIETVRADAGTRMMNFDPFRPLHRVA
                     LNDADGSQLHISAKTGEVVRVTTTISRTLTWLGSWLHFFRPLDDIGLGEQRKDILTWS
                     AFAACFAVLTGLWVGWQRWRPGWFGQRRYGSGQAHPYRERWPRWHFWLGLTGGGWAML
                     WIFSGFLANNPWHLFSHASVRPAELARYHGGGLPAAALDWRAPLATPSVELHWRRLGE
                     HAVLLAYQADGRVSRLSEFGAPQAASADRPAFSADELTRAALRLYPGAQVTQHNWLSE
                     GDAYYYPRPRGDDALDKPFPVLRLQLNDASASWLYLDPATGQLLKRVDRSRRAYRWLF
                     NALHSWDVPVLRSRPLWDGWMIIASVLGIGLGVTSLVVGGKRLARSWPWRRIR"
BASE COUNT          398 a          725 c          753 g          353 t
ORIGIN      
        1 tgcaggaagg tttccaccga gttcttgcgg cggtatttca gcgcggaatc cagcagcgcc
       61 tgattttgcg cgcccacgcc aatcgtgccg cggccgtcat cctggccgcc cagcacgctg
      121 agcagatggc cgatttcttc gttatccatc tcgccgtcgg cggacatcat gtacagcagc
      181 gaggtggcga aggccagatg cggggtcatt ttttcgccgc tgtcgctctt gaacatgtca
      241 aacaggccca ttgggtgctc tccttggtgt gtggtcaggg ggataaaagc ccggccgcgg
      301 caaacatatg cctacaacgg ccaggcaacg cgttagacca tggggtgttt agccaggttc
      361 cagcatgaaa ataccaacgg cccgcacatg ggcgggccgt ggtggggcac gccgccatcg
      421 tcgggggcag cgcgccggaa ttggtgcagg gagttcggac attccccgcc tgatgtcatc
      481 caagccccgg ccagctgtct gcaaccaccc tcgcaaaccg cgtttcgggg caggggccgt
      541 acttgagatg gcgagatatt agcgagtaga gattgcatgg cgatgacgat cacgttgcca
      601 ctggatggct tggcgcagat ctcagcgaat gcgtcgccac ggccacgaac gcgccagccg
      661 cttgccgccc accaccagcg acgtgacgcc caggccgatg cccagcacgc tggcgatgat
      721 catccagccg tcccacagcg ggcggctgcg caacaccggc acatcccagc tgtgcagggc
      781 attgaacagc cagcggtagg cgcggcggct gcggtccacc cgcttgagca gttggccagt
      841 ggccgggtcg aggtagagcc agctggcgct ggcgtcgttc agttgcaggc gcagcaccgg
      901 aaacggcttg tccagcgcat cgtcgccgcg cgggcgcggg tagtaatagg cgtcgccttc
      961 gctcagccag ttgtgttgtg tcacctgggc gccgggatac aggcgcaggg cggcgcgcgt
     1021 cagttcatcg gcgctgaagg ccgggcggtc tgcgctggcg gcttggggcg cgccaaattc
     1081 gctcagacgg ctgacgcggc catcggcctg gtaggccagc aacaccgcgt gttcgcccag
     1141 gcggcgccag tgcagctcca ccgacggcgt ggccagcggc gcgcgccagt ccagcgcggc
     1201 agccggcaag ccgccgccgt ggtagcgcgc cagctcggcc ggcctgacgc tggcgtggct
     1261 gaacagatgc caggggttgt tggccagaaa gccgctgaaa atccacagca tcgcccagcc
     1321 gccgccggtc agccccagcc agaaatgcca gcgcggccag cgttcacgat agggatgcgc
     1381 ctgaccgctg ccatagcggc gctggccaaa ccagcccggc cgccagcgct gccaacccac
     1441 ccacaggccg gtcagcacgg caaagcaggc ggcgaaggcg ctccaggtga gaatgtcctt
     1501 gcgttgctcg cccaggccga tgtcgtccag cggccggaaa aaatgcagcc agctgcccag
     1561 ccaggtcagc gtgcggctga tggtggtggt gacgcgcacc acttcgccgg ttttggcgga
     1621 gatatgcagc tggctgccgt cggcgtcatt cagcgccacg cggtgcaagg ggcggaaagg
     1681 gtcaaaattc atcatgcgcg tgccggcatc ggcgcgcacg gtttcaataa aactcggtgt
     1741 ggcggcgcgg cctgcggcgc gcgcccagtc ttgcgccagc cgcacggcgc gcgccgcgtc
     1801 ggtggagacg cgccggccat ccacggcaga cagcatgtgg cgctggccag ccagatcaat
     1861 caatagccac attggctcgc cggcttgcat catcagccgc gcctcagccg gcgcctcatc
     1921 ggcgcgttcg ggccaggcgc gccgccaggc ctggccggcg ctcagcggcg gggcggattc
     1981 gggcagagct tggctgtgcg ccagccgggt gagactgtca acacgggatg cgccggcata
     2041 gatcatgacc aggccggaga aaaaccagat aagcatgaac aggcccaggg cgatgcccag
     2101 ccagcggtgg aggataaaaa gcgtgcgttt caccaacgtt cctgacaatg tcgcgctcac
     2161 gcgcggattt gatgttgggc ggggcttcgc cccgcacccg accccttttc tttgtctcgc
     2221 caaagaaaa
//
LOCUS       DAOPYX010000394         2212 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_72168_length_2212_cov_13.885489, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000394 DAOPYX010000000
VERSION     DAOPYX010000394.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 2212)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2212)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2212
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72168_length_2212_cov_13.885489"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..479
                     /locus_tag="PKN55_13780"
     CDS             <1..479
                     /locus_tag="PKN55_13780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011136575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3390 domain-containing protein"
                     /protein_id="HNX72363.1"
                     /translation="CMNHCPVYSRIGGHAYGTVYPGPIGKIISPHMLGLNDTQDLPTA
                     SSLCGACGEVCPVRIPIPEMLMRLREEAARDPEEKVPHPLAGQGAAYSPQLARVWSLW
                     RTVYSRPALYRAFSWLATRLRVFTPRRQLGWTVHRTPLTPAKQSLHELVRQRQSSR"
     gene            489..>2212
                     /locus_tag="PKN55_13785"
     CDS             489..>2212
                     /locus_tag="PKN55_13785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018150928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding and (Fe-S)-binding domain-containing
                     protein"
                     /protein_id="HNX72364.1"
                     /translation="MLPAQYQAFYDQLTAHIDPARLITDPTRTLAYGTDASFYRLVPK
                     IVAQVNSESEIAALLALAHQHRVAVTFRAAGTSLSGQAVTDSVLVTLGAGWTGQEVLD
                     GGARIRLQPGVIGQHANNTLAPLGRKIGPDPASIATARIGGMAANNSSGMCCGTAQNS
                     YHTLSALRVMLADGTVLDTGDAASVTAFRASHATLLDGLSALAARIHGNPALAEKIRH
                     KYRLKNTTGYGINALLDFTDPVDMLSHVMIGSEGTLGFISSITFDTVPDYPHKASCLV
                     LFDELDPCCRAVTALKADAPVEAVELIDSRGIRAVQNKAGLPDFLYGEIGPSAAALLI
                     ETRAPSAEALAEQMAAIDACVAGFAPAANSGFSTDSQTCDTYWAVRKGLFPAVGAVRP
                     VGTTVVIEDVAFPVEALAEGVRRLTVLFDQYQYHEALIFGHALEGNLHFVFTPSFDSS
                     AEVKRYNDFMDAVSELVAVEFGGSLKAEHGTGRNVAPFVELEWGSDAYAIMRDIKKLF
                     DPHGVLNPDVIITDNDQLHLENLKAMPAADPLVDRCIECGFCEPACPSNGLSLTPRQR
                     IVLWRRIN"
BASE COUNT          374 a          769 c          711 g          358 t
ORIGIN      
        1 cctgcatgaa ccattgcccg gtgtacagcc gcattggcgg ccacgcctac ggcacggtct
       61 accccggccc gattggcaag atcatctcgc cgcatatgct tggcctgaac gacacccagg
      121 atttgcccac cgcctccagc ttgtgcggcg cctgcggcga agtgtgtccg gtgcgcattc
      181 ccatcccgga aatgctgatg cgcctgcgcg aagaagccgc gcgcgacccg gaagaaaaag
      241 tcccgcatcc gctggctggt caaggcgcgg cgtattcgcc gcaactggcg cgggtgtggt
      301 cgctgtggcg cacggtgtac agccgcccgg cgctgtaccg cgccttcagc tggctggcca
      361 cccgcctgcg cgtgttcacc ccgcgccgcc aactgggttg gacggttcac cgcacgccgc
      421 tgaccccggc caagcaatcg ctgcacgaac tggtgcgcca gcgccagtcg tcccgttgag
      481 gagcccgcat gctgcccgct caatatcagg cgttttatga ccagctgacc gcccacatcg
      541 acccggcgcg gctgatcacc gaccccaccc gcaccctggc ttacggcact gacgccagct
      601 tttaccggct ggtgcccaag atcgtcgccc aggtgaacag cgaaagcgaa atcgccgccc
      661 tgctggcgct ggcccaccag caccgcgtgg cggtgacctt ccgcgccgcc ggcaccagct
      721 tgtctggcca ggcggtgacc gattcggtgc tggtgaccct gggcgccggc tggaccggcc
      781 aggaagtgct ggacggcggc gcgcgcattc gcctgcaacc gggggtgatt ggccagcacg
      841 ccaacaacac cctggccccg ctgggccgca agatcggccc cgatccggcc tcgatcgcca
      901 ccgcgcgcat tggcggcatg gcggccaata attcttcggg catgtgctgc ggcaccgcgc
      961 aaaacagcta ccacacgctg tcggcgctgc gggtgatgct ggccgacggc acggtgctgg
     1021 acactggcga cgccgccagc gtgacggcgt ttcgcgccag ccacgccacg ctgctggatg
     1081 gcctgtcggc gctggcggcg cgcattcacg gcaacccggc gctggccgag aaaatccgcc
     1141 acaaataccg gctgaaaaac accaccggct acggcatcaa cgccctgctg gacttcaccg
     1201 acccggtgga catgctcagc catgtgatga tcggctcgga aggcacgctg ggctttatca
     1261 gcagcatcac cttcgacacc gtgcccgact acccgcacaa ggcgtcttgc ctggtgctgt
     1321 ttgacgagct ggacccgtgc tgccgcgcgg tgaccgcgct gaaggccgat gcgccggtag
     1381 aagcggtcga gctgatcgac agccggggca ttcgcgccgt gcaaaacaaa gccggcctgc
     1441 cggacttttt atatggcgag attggcccgt cggcggccgc gctgctgatc gaaacccgcg
     1501 cgcccagcgc cgaggcgctg gccgagcaaa tggcggccat cgacgcctgc gtggccggct
     1561 ttgctccggc ggcgaacagt ggtttctcca ccgacagcca aacctgcgac acctactggg
     1621 cggtgcgcaa gggcctgttc ccggcggtgg gcgcggtgcg cccggtcggc accacggtgg
     1681 tgatcgagga tgtggccttc ccggtggaag cgctggctga aggcgtgcgc cggctgaccg
     1741 tgctgtttga ccaataccag taccacgagg cgctgatttt tggccacgcg ctggaaggca
     1801 atctgcactt tgtgttcacc ccgagctttg actccagcgc cgaggtgaaa cgctacaacg
     1861 acttcatgga cgcggtcagc gagctggtgg cggtggagtt tggcggctcg ctcaaggccg
     1921 agcacggcac cggccgcaat gtggcgccgt ttgtcgagct ggaatggggc agcgacgcct
     1981 acgccatcat gcgcgacatc aaaaagctgt tcgatccgca cggcgtgctc aacccggacg
     2041 tgatcatcac cgacaacgac caactgcacc tggaaaacct caaggccatg ccggcggccg
     2101 acccgctggt ggaccgctgc atcgagtgcg gcttttgcga gccggcctgc ccgtccaacg
     2161 gcctgagcct gaccccgcgc cagcgcattg tgctgtggcg gcgcatcaac ca
//
LOCUS       DAOPYX010000395         2208 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_72362_length_2208_cov_12.347887, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000395 DAOPYX010000000
VERSION     DAOPYX010000395.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 2208)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2208)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2208
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72362_length_2208_cov_12.347887"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..643)
                     /locus_tag="PKN55_13790"
     CDS             complement(<1..643)
                     /locus_tag="PKN55_13790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008952253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HNX72365.1"
                     /translation="MTHNRTISVVGLGYVGLPVAVAFGKHARTIGFDINATRIAELKT
                     GHDRTGEVEDADLAAAQIVYTDQFDVLREADFHIVAVPTPVDDAHQPDLTPVEKASET
                     VARALKKGDIVVYESTVYPGVTEDICVPILEKLSGLRCGEDFTVGYSPERINPGDKEH
                     TFTKIKKVVSGQDAATLDIVAAVYASVVTAGVHKAASIKVAEAAKVIENTQRDL"
     gene            complement(806..1294)
                     /locus_tag="PKN55_13795"
     CDS             complement(806..1294)
                     /locus_tag="PKN55_13795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX72366.1"
                     /translation="MLSGLAQAHSLDGLLDSVASACQASPALVTLLQSLGQREGEPAQ
                     VRQPEQVAAPAELREAFAAPDRVAINEDTWLLGVAARGDWHGVEVTALTRTLSLSEPG
                     GEFAIELSGSPLEVQAALGKHLAFQHSKVAVDGVWQARGAQFVDGVNGENSTALVCPL
                     PF"
     gene            complement(1330..1869)
                     /locus_tag="PKN55_13800"
     CDS             complement(1330..1869)
                     /locus_tag="PKN55_13800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012698366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HNX72367.1"
                     /translation="MKRNLRPFDGIHPQLPESCFVDDTALIIGDAVLGEDCSVWPMAV
                     IRADVNSVRIGARSNVQDFCMLHESHKNDKDPNGAPLTIGEDVTIGHHVNLHGCTIGD
                     RVLVGMGTIVLDKAVVESDVIIGAGSLVPPGKRLASGYLYVGSPVKQARELTAEEREF
                     LRYSAAHYVRLANKHKAGQ"
     gene            2003..>2208
                     /locus_tag="PKN55_13805"
     CDS             2003..>2208
                     /locus_tag="PKN55_13805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HNX72368.1"
                     /translation="MDNPLLDFTALPRFADVRPDHITPAVDQVLANARAVVAAVTQPD
                     TPASWDALMAPLADATEKISWVWDP"
BASE COUNT          404 a          713 c          677 g          414 t
ORIGIN      
        1 tgaggtcgcg ctgggtgttt tcgatcacct tggcggcttc ggccaccttg atcgacgcgg
       61 ccttgtgcac gccagcggtc accaccgagg cgtagacggc ggcaacgatg tccagcgtgg
      121 cggcgtcctg gccggacacc acttttttga tcttggtgaa ggtgtgttcc ttgtcgcccg
      181 gattgatgcg ctcggggctg tagccaacgg tgaaatcttc accgcagcgc aggccggaga
      241 gtttttccag aatcggcacg cagatgtctt cagtcacgcc gggatatacg gtggattcgt
      301 acaccacgat atcgcctttt ttcagcgcgc gcgccacggt ttcgctggct ttttccaccg
      361 gggtgaggtc gggctggtgg gcgtcgtcta ccggggtggg cacggccaca atgtgaaaat
      421 cggcttcgcg cagcacgtcg aattggtcgg tgtagacaat ctgggcggcg gccaggtcgg
      481 cgtcttccac ttcgccagtg cggtcgtggc cggttttcag ttcggcaatg cgggtggcgt
      541 tgatgtcaaa gccgatggtg cgcgcgtgct tgccaaaggc gacggccacc ggcaggccca
      601 catagcccag gccaaccacg gagatggtgc gattgtgtgt catgctgaat catcctggcg
      661 caagcaccgg ttttgccagc gccacgccca ctgcgattga gagaaaaaaa caaaaaatcg
      721 ggccggcgtt aaggccggcg gtgttggcgc tattgtggcg tgaatccgcc agccacgcca
      781 gttatgtcat cagggttttt actgatcaaa acggcagcgg gcagaccagc gccgtgctgt
      841 tctcgccatt cacgccatcg acaaattgcg cgccacgcgc ctgccagacg ccgtctaccg
      901 ccactttgct atgctgaaac gccaaatgtt tgcccagtgc ggcctgcact tccagcggcg
      961 agccgctaag ttcaatggca aactcgcccc ccggctcgct caggctgagc gtgcgcgtca
     1021 gcgcggtgac ttccacaccg tgccagtcgc cacgggcagc cacccccagc agccaggtgt
     1081 cctcgttgat ggccacccgg tccggcgcgg caaacgcctc gcgcagctcg gccggagcgg
     1141 ccacctgctc gggctggcgc acttgcgccg gctcgccctc gcgctgcccc agcgattgca
     1201 gcaatgtcac cagggccggg ctggcctggc aggcgctggc cacgctatcc agcaggccat
     1261 ccagagaatg cgcttgtgct aaaccactga gcattgcggc cgataacaat aaagttcgcc
     1321 atgcgcgcat cactggcctg ccttgtgctt gttggccagg cgcacatagt gtgcggcgga
     1381 atagcgcaaa aattcgcgct cttcggcagt caattcgcgc gcctgcttca ccggcgagcc
     1441 cacatacaaa tagcccgacg ccaggcgttt gcccggcggc accaggctgc cggcgccgat
     1501 gatcacatcg gactccacca cggccttgtc cagcacgata gtgcccatgc caaccagcac
     1561 gcggtcgcca atggtgcagc cgtgcagatt gacatggtgg ccaatggtga catcctcgcc
     1621 gatggtcagc ggcgcgccgt tggggtcttt gtcgttcttg tgcgattcgt gcagcatgca
     1681 aaaatcctgc acattgctgc gcgcgccgat gcgcaccgaa ttgacgtcgg cgcggatcac
     1741 cgccatcggc cacaccgagc aatcctcgcc cagcacggca tcgccgatga tcagcgcggt
     1801 gtcgtcaaca aaacaggatt cgggcagctg cgggtggatg ccgtcaaacg ggcgaagatt
     1861 gcgtttcatt cagaaagacc ccatatcaaa gcgtggcgtg aacatccgct atcataaccc
     1921 gaagcgcatg acgccgcgcg ccgccgcgcc gaccggcgcg gcaattgtgc caaacgactg
     1981 atttattgaa ggatgcctga ggatggataa cccgctgctc gactttaccg cactgccccg
     2041 ctttgccgat gtccgtcccg accatatcac cccggcggtg gaccaggtgc tggcgaatgc
     2101 ccgcgccgtg gtggccgccg tcacccagcc cgacaccccg gccagctggg acgccctgat
     2161 ggcgccgctg gccgacgcca ccgaaaaaat cagctgggtg tgggaccc
//
LOCUS       DAOPYX010000396         2203 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_72653_length_2203_cov_12.885940, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000396 DAOPYX010000000
VERSION     DAOPYX010000396.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 2203)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2203)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2203
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72653_length_2203_cov_12.885940"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..755)
                     /locus_tag="PKN55_13810"
     CDS             complement(<1..755)
                     /locus_tag="PKN55_13810"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNX72369.1"
                     /translation="MSFVRFENVDLAYAPGGALAVQGLNLDIAEGEFVAVVGPSGCGK
                     STLMKLASGLRPPSNGYVFVDGREVGGPLKIVGMAFQAANLLPWRSTLDNVMLPLEIV
                     EPHRGLLRKQRAEYEAQARALLARVGLDGYADKHPWELSGGMQQRASICRALIHQPRL
                     LMLDEPFGALDAFTREELWCMLRDLWQEKPFTVILVTHDLREAVFLADTVYVMSARPG
                     RVLLRHPIDLPRPRDLDLTYTDAFAQHVHALRE"
     gene            complement(766..1794)
                     /locus_tag="PKN55_13815"
     CDS             complement(766..1794)
                     /locus_tag="PKN55_13815"
                     /inference="COORDINATES: protein motif:HMM:NF020650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNX72370.1"
                     /translation="MLTRLHVCRRWFLAASLALMAAPTLAEPLPKVKFTLDWRFEGPS
                     APFLLAKAKGYFQAEGVDVEIDSGSGSAGAVTRVATGAYEMGFADFNALVEYDAKTPG
                     SKIQAVYMVYNATPAAVLTLKKTGISKPADLVGKTLAAPVFDAGRKAWPAFAKSNKLD
                     PASVKWQTVEPALRETLLARGDVDAITGFYFTSVLNLEARGVKPEDIVALKYPDHGVE
                     LYGNAIIASPKLLAENPKAVSAVLRGFNRALKDTLANPAAAIAYVKERDPLINVPLET
                     RRLKLALDHSVLTAEVRANGLGAVTAERMQRSLAETAEAYGLSRVPAAAELFNPAFLP
                     ARAERMVK"
BASE COUNT          426 a          676 c          694 g          407 t
ORIGIN      
        1 tgctcgcgca gggcgtgcac atgctgggca aaggcgtcgg tgtaggtcag gtcaaggtcg
       61 cgcgggcgcg gcaggtcgat ggggtggcgc agcagcacgc ggccgggccg ggcgctcatc
      121 acatacacgg tgtcggccag aaacaccgct tcgcgcaggt cgtgggtgac cagaatcacg
      181 gtgaagggtt tttcctgcca caggtcgcgc aacatgcacc acagctcttc gcgggtgaag
      241 gcgtccagcg cgccaaacgg ctcgtccagc atcaacaggc gcggctggtg aatcagcgcc
      301 cggcagatcg aggcgcgctg ttgcatcccg ccggacaatt cccacgggtg tttgtcggcg
      361 tagccatcca gccccacccg cgccagcagc gcgcgcgcct gtgcttcata ttcggcgcgc
      421 tgtttgcgca gcaggccccg atgcggctcg acgatttcca gcggcagcat cacattgtcc
      481 agcgtgctgc gccacggcag cagattggcg gcctgaaacg ccatgccgac gattttcagc
      541 gggccgccca cttcgcggcc atcgacaaac acatagccgt tgctgggcgg acgcaggcca
      601 gaggccagtt tcatcagggt ggatttgccg cagccggacg gacccaccac ggcgacaaat
      661 tcaccctcgg caatgtccag gttcagcccc tgcaccgcca gcgcgccgcc tggcgcgtag
      721 gccagatcga cattttcaaa acgcacaaac gacatgcccg gccccttatt tcaccatccg
      781 ctcggcgcgc gccggcagga aggcggggtt gaacagctcg gcggcggccg gcacgcggct
      841 caggccgtag gcttcggcgg tttccgccag cgagcgctgc atgcgctcgg cggtgaccgc
      901 gcccaggcca ttggcgcgca cttcggcggt cagcacgctg tggtccagcg ccagtttcag
      961 ccggcgggtt tccagcggca cattgatcag cgggtcgcgc tctttcacat aggcaatcgc
     1021 ggcggccggg ttggccaggg tgtctttcag cgcgcggtta aagccgcgca gcacggcgct
     1081 gactgctttg ggattctcgg ccagcagctt ggggctggcg atgatggcgt tgccgtacag
     1141 ctccacgccg tgatccgggt atttcagcgc gacgatgtct tctggcttca ccccgcgcgc
     1201 ttccagattg agcacgctgg tgaaataaaa gccggtgatg gcgtccacat cgccgcgcgc
     1261 cagcagggtt tcccgcagcg ccggctcgac ggtttgccat ttcacgctgg ccgggtccag
     1321 cttgttggat ttggcaaacg ccggccaggc cttgcgcccg gcgtcgaaca ccggcgcggc
     1381 cagggttttg cccaccaggt cagccggctt gctgatgccg gtttttttca gggtcagcac
     1441 cgcagccggg gtggcgttgt acaccatata caccgcctgg attttcgagc cgggggtttt
     1501 cgcgtcgtat tccaccaggg cgttgaaatc ggcaaacccc atctcatacg cgccagtggc
     1561 cacgcgggtg acggcgccgg ccgaacccga gccggaatca atttcgacgt ctacgccttc
     1621 cgcctgaaaa taccctttgg ccttggccag taaaaacggc gccgacgggc cttcaaatcg
     1681 ccagtccagg gtgaatttca ccttgggcaa aggctcggcc agggtggggg cggccatcag
     1741 cgccaggctg gcggctaaaa accaacggcg gcaaacgtgc aggcgggtga gcatggggca
     1801 actccagaaa ggggcggaaa gcccgctttt tgcaagtcgc atgccagcat acaagacact
     1861 gatttacaaa gaaaaataaa acacagttat gccaaaagaa aatttggcgc aaaaataagc
     1921 gcaatacacc ggccaaatca gcgctttgcc gtgcatttat ttggtgcgcg aggatgtttt
     1981 ttgcctcatt caggtgcgcg cttgggcaat attttaaatc agatacgcgg ggtatattga
     2041 atcgccaata gggccgggat tatgtgattt tgtttatgcg gggtatatct gcacccggcg
     2101 ggaaaatccg gttaaatgca gctcatccgc cccatattgc tgtgcaacat acggcagcgg
     2161 ggtcgagaat aagcgccagc acaaggcgcg aacaacgatt agc
//
LOCUS       DAOPYX010000397         2200 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_72814_length_2200_cov_9.149184, whole genome shotgun sequence.
ACCESSION   DAOPYX010000397 DAOPYX010000000
VERSION     DAOPYX010000397.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 2200)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2200)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2200
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72814_length_2200_cov_9.149184"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1677
                     /gene="argS"
                     /locus_tag="PKN55_13820"
     CDS             <1..1677
                     /gene="argS"
                     /locus_tag="PKN55_13820"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008953698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HNX72371.1"
                     /translation="LAAAGCPDASPILQTASRAEFGDYQANGIMAAAKAKKTNPRALA
                     EQVIAQLDLAGIASKIEIAGPGFINIHLDPAFLAGKVSAALASPSLGIEPQSPRQKVI
                     VDYSSPNLAKEMHVGHLRSSIIGDALVRVLAAIGHDTIRCNHVGDWGTQFGMLTAYLV
                     ESENQDAGLALNDLEDFYRKAKIRFDEDAAFADRARDYVVRLQSGDAEVLALWKQFVD
                     VSLTHCEAVYRKLGVSLTRADARGESAYNADLPVIVRELREKGLLAEDDGAQVVFLEE
                     FRNQDGDPMGVIIQKKDGGYLYTTTDLGAVRYRCRELGVDRVLYVVDARQSQHFQQVF
                     TLCRKAGFAPEHVQLQHIGFGTMMGEDGKPFKTRSGGTVKLIELLDEAEERAFQLVSE
                     KNPDLDDAARRRVALAVGIGAVKYADLSKNRNSDYIFNWDQMLAFEGNTAPYLQYAYT
                     RVASLFRKAGEHDANAPVQIDAAEEKALAVALAQFNDVLYHVADEGYPHFLCQYLYQV
                     STLFMRFYEACPVLKSEGAVRASRLQLARLTAATLKTGLDLLGIEVLEAM"
     repeat_region   1806..2193
                     /inference="COORDINATES: alignment:crt:1.2"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=1866..1893
                     /rpt_unit_seq="tttctcagctgcctattcggcagttcac"
BASE COUNT          443 a          738 c          626 g          393 t
ORIGIN      
        1 ctggccgccg ctggctgccc ggacgcctcg cccattctgc aaaccgcctc gcgcgccgag
       61 tttggcgact atcaggccaa cggcatcatg gccgccgcca aagccaaaaa aaccaacccg
      121 cgcgcgctgg ccgagcaagt gattgcccag ctggacctgg ccggcatcgc cagcaaaatc
      181 gaaatcgccg gccccggctt tatcaatatc cacctcgacc cggcgtttct ggccggcaag
      241 gtcagcgccg cgctggccag ccccagcctg ggcatcgagc cgcaaagccc gcgccagaag
      301 gtgattgtcg attactcctc gcccaacctg gccaaagaaa tgcacgtcgg ccacctgcgc
      361 tccagcatca tcggcgatgc gctggtgcgc gtgctggccg ctatcggcca cgacaccatt
      421 cgctgcaacc acgtgggcga ctggggcacc cagtttggca tgctcaccgc ctacctggtg
      481 gaaagcgaaa accaggacgc cggcctggcg ctgaacgacc tggaagactt ttaccgcaag
      541 gccaaaatcc gctttgacga agacgccgcc tttgccgacc gcgcccgcga ctatgtggtg
      601 cgcctgcaat ccggcgacgc cgaagtgctg gcgctgtgga agcaatttgt cgatgtctcg
      661 ctcacccact gcgaagcggt gtaccgcaaa ctgggcgtca gcctcacccg cgccgacgcg
      721 cgcggtgaat ccgcctacaa cgccgacctg ccggtgatcg tgcgcgaact gcgcgaaaaa
      781 ggcctgctgg ccgaagacga cggcgcgcaa gtggtgtttc tggaagaatt ccgcaaccag
      841 gacggcgacc cgatgggcgt catcatccag aaaaaagacg gcggctatct ctacaccacc
      901 accgacctgg gcgcagtgcg ctaccgctgc cgcgaactgg gcgtggaccg cgtgctgtat
      961 gtggtggacg cccgccagag ccagcatttt cagcaagtgt tcaccctgtg ccgcaaggcc
     1021 ggctttgccc cggaacacgt ccagctgcaa cacatcggct ttggcaccat gatgggcgaa
     1081 gacggcaagc cgtttaaaac ccgcagcggc ggcaccgtca aactgatcga actgctggac
     1141 gaagccgaag aacgcgcctt ccagctggtc agcgagaaaa accccgacct ggacgacgcc
     1201 gcccgccgcc gcgtggcgct ggccgtgggc atcggcgcgg tgaaatacgc cgacctgtcg
     1261 aaaaaccgca acagcgatta catcttcaac tgggaccaga tgctggcctt tgaaggcaac
     1321 accgcgccct atctgcaata cgcctacacc cgcgtggcca gcctgttccg caaggccggc
     1381 gaacacgacg ccaacgcccc ggtgcagatc gacgccgccg aagaaaaagc cctggccgtc
     1441 gccctggcgc agtttaacga cgtgctctac cacgtcgccg acgaaggcta cccgcacttc
     1501 ctctgccaat acctgtacca ggtgtccacc ctgttcatgc gcttttacga agcctgcccg
     1561 gtgctgaaaa gcgaaggcgc cgtgcgcgcc tcccgcctgc aactggcccg cctgacggca
     1621 gcgacgctga agaccgggtt ggatttgctg gggattgagg tgttggaggc gatgtaacaa
     1681 accaccagga taggcggcgg atttgctggc tgtttgcaaa caacgtattc aacagcaaaa
     1741 ccgccccgtc ctcacccgca gctcatctca atgaccggtc ggcctctcca gccatggcaa
     1801 atgtagagag ctgctgccta ttcggcagtt cacgtggatg cgcagcgggt tgaatggctg
     1861 gtgcatttct cagctgccta ttcggcagtt cacgctgcaa tcaacagccc gcgcacgcaa
     1921 gagattttct cagctgccta ttcggcagtt cacaaagggc tttcgctggt caacgctgcc
     1981 ggccatttct cagctgccta ttcggcagtt cacttgtgct ggcagtggtg cgcgcaagcg
     2041 ggtggtttct cagctgccta ttcggcagtt caccattgcc tcgttcagtg cgtccagctc
     2101 atccgtttct cagctgccta ttcggcagtt cactgctcca cgcggtgagc tgatcatcag
     2161 caacatttct cagctgccta ttcggcagtt cacgacgcta
//
LOCUS       DAOPYX010000398         2165 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_74598_length_2165_cov_9.377251, whole genome shotgun sequence.
ACCESSION   DAOPYX010000398 DAOPYX010000000
VERSION     DAOPYX010000398.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 2165)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2165)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2165
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74598_length_2165_cov_9.377251"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1979
                     /locus_tag="PKN55_13825"
     CDS             <1..1979
                     /locus_tag="PKN55_13825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003766015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNX72372.1"
                     /translation="NPVLIGEPGVGKTAIVEGLAQRIVNGEVPESLKHKKLLVLDLAA
                     LLAGAKFRGEFEERLKSVLNELAKDEGNTIVFIDEIHTLVGAGKAEGAMDAGNMLKPA
                     LARGELHCLGATTLDEYRKYIEKDAALERRFQKVLVDEPSVENTIAILRGLREKYEIH
                     HGVDITDPAIVAAAELSHRYITDRFLPDKAIDLIDEAASRIKMEIDSKPESMDKLERR
                     MIQLKIEREAVRREKDEASQKRLSLIEDEIRRLEREYSDLEEIWKAEKAAVQGSQSIK
                     EQLDRLRIDMEDATRRGDWQKVSELQYGKLPQLEAQLKAAEAGENPQSSKTKLLRTQV
                     GAEEIAEVVSRATGIPVSKMLSGEREKLLKMEDVLHQRVVGQDEAVRVVADAIRRSRS
                     GLSDPNKPYGSFLFLGPTGVGKTELCKTLAGFLFDSEEHLIRIDMSEYMEKHSVARLI
                     GAPPGYVGYEEGGYLTEQVRRKPYSVILLDEVEKAHPDVFNILLQVLDDGRLTDGQGR
                     TVDFRNTVVVMTSNMGSQLIQSMATDDYELVKLAVMGEVRTHFRPEFINRIDETVVFH
                     ALDSQHIASIARIQLKHLQARLAAMDLQLDISEAALALICEAGFDPVYGARPLKRAIQ
                     SEIENPLAKAILAGRYPPKTVIHVGEANQRLVFA"
BASE COUNT          483 a          655 c          684 g          343 t
ORIGIN      
        1 acaacccggt gctgattggc gaaccgggcg tgggcaagac ggcgattgtc gaaggcctgg
       61 cccagcgcat tgtcaatggc gaagtgccgg aaagcctcaa gcacaaaaag ctgctggtgc
      121 tggacttggc cgcgctgctg gccggcgcca agttccgtgg cgaatttgaa gaacggctga
      181 agtcggtgct caatgagctg gccaaagatg aaggcaacac catcgtcttt atcgatgaaa
      241 tccacaccct ggtgggcgcc ggcaaggccg aaggcgccat ggacgccggc aatatgctca
      301 agccggcgct ggcgcgcggc gagctgcact gtctgggcgc caccacgctg gacgagtacc
      361 gcaagtacat cgaaaaagac gccgcgctgg agcgccgctt ccagaaggtg ctggtggacg
      421 agccgagcgt ggaaaacacc atcgccatct tgcgcggcct gcgggagaaa tacgaaattc
      481 accacggcgt ggacatcacc gacccggcga ttgtcgccgc cgccgagctg agccaccgtt
      541 acatcaccga ccgctttttg ccggacaagg ccatcgacct gatcgacgaa gccgccagcc
      601 gcatcaagat ggaaatcgac tccaagccgg aatccatgga caagctggag cggcgcatga
      661 ttcagctcaa gatcgaacgc gaagcggtgc gccgggaaaa agacgaagcc tcgcaaaagc
      721 gcctgagcct gatcgaagac gaaatccgcc ggctggagcg tgaatattcc gacctggaag
      781 aaatctggaa agcagaaaaa gctgccgtgc agggttcgca gtcgatcaaa gaacagctcg
      841 accgcctgcg catcgacatg gaagacgcca cccgccgtgg cgactggcaa aaagtctccg
      901 agctgcaata cggcaagctg ccgcagctgg aagcacaact gaaagccgcc gaggctggcg
      961 aaaacccgca aagcagcaag accaagctgc tgcgcaccca ggtgggcgcc gaggaaatcg
     1021 ccgaagtggt cagccgcgcc accggtattc cggtatccaa gatgctgtct ggcgagcggg
     1081 aaaaactgct gaaaatggaa gacgtgctgc accagcgcgt ggtgggccag gacgaggccg
     1141 tgcgcgtggt ggccgacgcc atccgccgct cgcgctccgg cctgtctgac ccgaacaagc
     1201 cgtatggttc tttcctgttc ctcggcccga ccggggtggg caagaccgag ctgtgcaaga
     1261 ccttggccgg cttcctgttt gacagcgaag aacacctgat ccgcattgat atgtcggaat
     1321 acatggagaa acactcggtc gcccgcctga ttggcgcgcc gccgggctat gtcggctatg
     1381 aagaaggcgg ttatctcacc gaacaggtgc gccgcaagcc gtatagcgtg attctgctcg
     1441 acgaggtgga aaaagcccac ccggacgtgt tcaatatcct gctgcaagtg ctggacgacg
     1501 gccggctgac cgacggccag ggccgcacgg tggatttccg caacaccgtg gtggtgatga
     1561 cctcgaatat gggcagccag ctgatccaga gcatggccac cgacgattac gagctggtca
     1621 agctggcggt gatgggcgaa gtgcgcaccc acttccgccc ggaattcatc aaccgcattg
     1681 atgaaaccgt ggtgttccac gcgctggaca gccagcacat tgcctcgatt gcccgcatcc
     1741 agctcaagca cctgcaagcg cggctggcgg cgatggactt gcaactggac atcagcgaag
     1801 cggcgctggc cttgatctgc gaggctgggt ttgatccggt gtatggcgcg cggccgctga
     1861 agcgggcaat tcagagcgaa atcgaaaacc cgctggccaa ggccattctg gctggccgtt
     1921 atccgcccaa gacggtgatt cacgtcgggg aggccaatca gcggctggtg tttgcctgac
     1981 cgacagcggc cacaaacgca aaaacgccac gggagaggcg ctcccgtggc gtttttgcgt
     2041 acagcacacc ccaaacaacc cggttcgggg ttaaccctcg gccagcagcg cagcggcttc
     2101 gcgcgccatc tgcccttctt cgtcggtggc gatcacccag gccgacaggc ggctgccgtc
     2161 gcggg
//
LOCUS       DAOPYX010000399         2163 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_74720_length_2163_cov_7.138046, whole genome shotgun sequence.
ACCESSION   DAOPYX010000399 DAOPYX010000000
VERSION     DAOPYX010000399.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 2163)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2163)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2163
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74720_length_2163_cov_7.138046"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(211..510)
                     /locus_tag="PKN55_13830"
     CDS             complement(211..510)
                     /locus_tag="PKN55_13830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX72373.1"
                     /translation="MPRAHKKLIIAPALAEHKLLQTPVFEHPAQHVKEPEQGGFAGAI
                     GPNQYRKRRQRQILDAAQAAKAFNADGFDAHGRWRLCLPANIGKLDHSALSTKER"
     gene            496..1383
                     /locus_tag="PKN55_13835"
     CDS             496..1383
                     /locus_tag="PKN55_13835"
                     /inference="COORDINATES: protein motif:HMM:NF024700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNX72374.1"
                     /translation="MGARQTPRIRAELCLEAPSGLYGYRFDLAHVAGGDSVILMNEAY
                     RDAPSAAQAEVEWTPIGAASKESCLWDRQERAAKTIRGLLQQCVVYQFHDTSPNSAIH
                     RRWDITDAVRLRPDGGNLAAVLLDLRHADSQRYALIVRQIRRVLPAFQDFVLEEQAGK
                     VLLRWQGAQSDKVFGSHLTSDGSLRVFCLLTLLNLPSARLPDVMLFDEPELGLHPHAI
                     TLVAEMFKRLSKKRQILIATQSPYLVDCFELENIIIASASHGETRLRTLPREQYQAWL
                     DDEYQLSDIWLKHSVGEME"
     gene            1380..2027
                     /locus_tag="PKN55_13840"
     CDS             1380..2027
                     /locus_tag="PKN55_13840"
                     /inference="COORDINATES: protein motif:HMM:NF025468.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4276 family protein"
                     /protein_id="HNX72375.1"
                     /translation="MTRVCIVCEGQTEVEFVKSCLAPYLLASHVRAFPSLLQSPSGSH
                     RGGRVTVARLVKFISHQYHHTDRITTLVDFYGFQDRNGRSRAQLEADIRAGIAAATAG
                     YDPRFVLPYVQMHEFEALLFTDPEAFAWVEDGWNEHSRAALKRVRQAFASPEDINDSP
                     ETAPSKRILSIFEAGAYSKTEHGPLIAEMIGIDAMREHCPQFNAWLTQLQAWGKQ"
BASE COUNT          423 a          612 c          649 g          479 t
ORIGIN      
        1 ggggaagttc gggttaaaag cgggggtggg cagtggaatg ctctgtggct ggcgcggatg
       61 cgtggggggc agaggtgatc gggctgcctg gcgcagtggg cgcaagggcc gtcagatcag
      121 gtctcggcgt ctgcatatca actgcaattc gtttttatca aaaactatcc gtttttatcg
      181 tttctgctct attctctatc actttgataa tcatctttct tttgtagaga gcgcgctatg
      241 gtcgagcttg ccgatattgg ccggcaaaca caggcgccaa cgcccgtgcg catcaaatcc
      301 gtcagcattg aaggctttcg cagcttgcgc tgcatccaga atttggcgct gccgccgctt
      361 gcggtattga ttggggccaa tggcgccggc aaatccacct tgctccggtt ctttgacatg
      421 ttgggctgga tgctcaaaga caggcgtctg caagagtttg tgctccgcca gggcgggggc
      481 gatgatcagt tttttatggg cgcgcggcaa acgccgcgca tccgcgccga gctgtgtctg
      541 gaagcgccgt cgggtttata cggctaccgc tttgatttgg cgcatgtcgc ggggggtgat
      601 tcggtcatct tgatgaatga ggcctatcgc gatgcgccaa gcgcggcaca ggctgaggtg
      661 gaatggacgc ccattggcgc cgccagcaaa gaatcctgcc tctgggatag gcaggaacgc
      721 gccgccaaga cgattcgcgg ccttttgcag cagtgcgttg tctaccagtt tcatgataca
      781 tcgcccaatt cagccatcca tcgtcgctgg gacatcactg acgctgttcg cctgcgcccg
      841 gacggcggca atcttgctgc ggttttgctg gatttgcgcc atgccgacag ccaacgctat
      901 gcactgattg tcaggcaaat tcgccgggtg ttgccggcct ttcaggattt tgtgctggaa
      961 gaacaagccg gcaaggtgtt gctgcgctgg cagggggcgc aaagcgacaa ggtgtttggt
     1021 tcgcacctga catcggatgg ctcgctgcgt gtgttctgcc tgctgacctt gctgaacctg
     1081 ccgtcagcgc gtttgcctga tgtgatgttg tttgacgagc ccgagctggg cttgcatccg
     1141 cacgccatca ccttggtggc ggagatgttc aagcgtttgt cgaaaaagcg gcaaatcctc
     1201 attgccacgc agtcgccgta tctggtggac tgtttcgaac tggaaaacat catcatcgcc
     1261 agcgccagcc acggtgaaac acgcctgcgc acattgccgc gcgaacagta tcaagcctgg
     1321 ctggatgatg aataccaatt gtctgacatt tggttaaagc acagtgtcgg ggagatggaa
     1381 tgacccgtgt ctgcatcgtc tgtgaggggc aaaccgaggt cgaatttgtc aaaagctgcc
     1441 tggcccctta cctattggca agccacgtgc gggcatttcc gtctttgctt cagtcgccat
     1501 ctggcagcca tcggggcggt cgcgtcaccg tcgcgcgttt ggtgaagttc atctcacacc
     1561 aatatcatca caccgaccgc atcaccacac tggtggattt ctatggattt caagatcgca
     1621 acggtcgcag ccgcgcccaa ctggaggcgg acattcgcgc cggcatagcc gccgccacag
     1681 cgggctatga tccgcgcttt gtcttgccct atgtgcagat gcatgagttt gaggccctgc
     1741 tgttcaccga ccctgaggca tttgcatggg tggaggacgg ctggaacgaa cacagccgcg
     1801 cagcgctcaa gcgtgtgcgg caagcctttg cctcgccaga agacatcaac gacagcccag
     1861 aaaccgcgcc atccaaacgc atcttgtcga ttttcgaagc aggcgcctac tcgaagaccg
     1921 agcatgggcc gctgattgcc gagatgattg gcattgacgc gatgcgcgag cactgccctc
     1981 agttcaacgc ctggctgaca caacttcagg cctggggaaa gcaataatgc accccgcaac
     2041 aatacctcag cgccaaggcg cgcagcgctg acttgagcgc ggcggcgttc acccgctcac
     2101 gccgacgccg gtgggctcac cggcgtgcac agctccacca gaaagccatt caggtcgctg
     2161 acg
//
LOCUS       DAOPYX010000400         2160 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_74858_length_2160_cov_5.866983, whole genome shotgun sequence.
ACCESSION   DAOPYX010000400 DAOPYX010000000
VERSION     DAOPYX010000400.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 2160)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2160)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2160
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74858_length_2160_cov_5.866983"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(436..1245)
                     /locus_tag="PKN55_13845"
     CDS             complement(436..1245)
                     /locus_tag="PKN55_13845"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HNX72376.1"
                     /translation="MPHFAANLSMLFTDLPWPERPAAAARAGFAGVEIQFPYEAIAAD
                     TLAAVCAEAGVRLCLINVPAGDLMQGGPGLAAVPGREAAFAQAVDACLAYLAALGAQA
                     PGRVNVLAGRLADGVSRTQALATLCANLRAALPRFQARGVTVGLEAINRHDMPGFLLN
                     TADDIAAVLAELGQPAGLDAQIDLYHMAREGVDLSALLRQPPAPIGHVQFADVPGRGA
                     PGTGALDWPTLFALVEQHCPEVWCAAEYRQASADQAQPALWQGWAAQGRGR"
     gene            complement(1299..1979)
                     /locus_tag="PKN55_13850"
     CDS             complement(1299..1979)
                     /locus_tag="PKN55_13850"
                     /inference="COORDINATES: protein motif:HMM:NF037888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX72377.1"
                     /translation="MANTKIPEDVSEIYEKIHAETVWLHHRWACYSELFRHTPRRIEL
                     LNASAPTFFFIVQELIERDVVLSFSRLTDPARDNRKNENISLDMLQERLKSLSAPESE
                     QFIQSCQELLGRLKIQCKPLLHLRRKVVAHFDLKVALCKHEENRLPNLSFQVIEDALG
                     TLRDYLNMIERYFSGNHGEYYFYPAGNNGGAESLIAFLRYGLRCEELLQQEKISPLDL
                     NQGDWHDA"
BASE COUNT          499 a          656 c          555 g          450 t
ORIGIN      
        1 ccggcacaca cccggccccc gctgactgca tgtattgtgc agcaccgggg gccggttgtt
       61 tttgtcggga gaataaaacc tgctgcggca atcggactgt actgacgcca cgctgatggc
      121 ccccgccaca ccaccgccca tccaaccggc gcggcaagcc tggctgagcg cgtggccgca
      181 gatggcgctt agcacactca attcgcccag tcgccagtca ccatgatctg aaccgactgg
      241 ccggtactta aaaccgttgt acgcagtgga cacgcctgga ttcccgcctt cgcgggaatg
      301 acccggtgtc gtgcggcgag gggttctttg tggaacatga accttcgtgt ttcaacgtga
      361 caggctacta gcgcggctga ccacacagct atttatctta cacctaagac agtccagcta
      421 cgccgccaaa ccgcctcagc gcccgcgtcc ctgcgccgcc cagccctgcc acagcgccgg
      481 ctgcgcctga tcggcgctgg cctggcgata ttccgccgcg caccacacct cgggacaatg
      541 ctgctcgacc agggcaaaca gcgtgggcca atccagcgcg ccggtgcccg gcgcgccacg
      601 gccaggtaca tcggcaaact gcacatggcc aatcggcgcg ggcggctgac gcaacaaggc
      661 tgacaaatcc accccttcgc gcgccatgtg atacagatca atctgcgcat ccagcccggc
      721 cggctgtccc agctcggcca gcacggcggc gatatcgtct gcggtgttca gcagaaaacc
      781 aggcatatcg tggcggttaa tcgcctccag ccccaccgtc accccccgcg cctgaaatcg
      841 gggcaaggcc gcgcgcagat tggcgcacag ggtggccagc gcctgcgtcc ggctgacccc
      901 atccgccagc cgcccggcca gcacattcac ccgccccggc gcctgcgcgc ccaaggccgc
      961 cagataggcc aggcacgcgt ccaccgcctg ggcaaacgcc gcctcgcggc ccggcaccgc
     1021 cgccaaaccc ggcccaccct gcatcaaatc gccggccggc acattgatca ggcataaacg
     1081 cacgccggcc tcggcgcaga cggcggccag ggtgtcggca gcgatggcct catacggaaa
     1141 ctggatttcc accccggcaa aaccggcgcg cgcggcggca gccgggcgct cgggccaagg
     1201 gaggtcggtg aacagcatgg agaggttggc ggcgaaatgg ggcatacgct tgaaattata
     1261 aattggttta tattatagag tttttttggg gcactttgtc acgcatcatg ccaatcccct
     1321 tggttaaggt ctaggggcga gattttttct tgttgaagaa gctcttcaca cctaaggcca
     1381 tagcgtaaaa aagcaatcag cgattctgca cctccattat taccagctgg gtaaaaatag
     1441 tattcaccat ggttgccact aaagtaacgc tcaatcatat tcaagtagtc tcgaagagtg
     1501 ccaagcgcgt cttcaataac ttgaaatgac aggttaggca gtctgttttc ctcatgtttg
     1561 caaagagcga cctttaaatc aaagtgcgca accactttgc gtcggagatg gagaagaggc
     1621 ttacactgaa ttttcaaccg gccaagcagt tcttggcagc tttgaataaa ttgctcggac
     1681 tctggggctg aaagtgattt caatcgttct tgtagcatat caagagaaat attttcattt
     1741 ttcctattat ccctagccgg atctgtaagc cggctaaacg acaaaaccac atcgcgctca
     1801 attaactctt gaacaataaa aaagaaggta ggcgctgatg cattaagcaa ttctatgcgc
     1861 cttggagtat gcctaaacaa ttcactatag cacgcccagc ggtgatgaag ccacacagtt
     1921 tctgcgtgaa ttttttcata tatttcacta acatcctctg gaattttagt gttagccatt
     1981 atttgcctta tcaaagaaac gatatatgta gtttaggtta ccagttcatt taaaataaca
     2041 attacatatt acggcacttg gcgcgcgaga ctttagtgtt actcaaaact tctcttggcc
     2101 gttgacaatg cccagcgtaa tgcacacctg tcacgtcccg tgtgatcata tccccatacg
//
LOCUS       DAOPYX010000401         2128 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_76609_length_2128_cov_10.664737, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000401 DAOPYX010000000
VERSION     DAOPYX010000401.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 2128)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2128)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2128
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76609_length_2128_cov_10.664737"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            171..572
                     /locus_tag="PKN55_13855"
     CDS             171..572
                     /locus_tag="PKN55_13855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014088493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HNX72378.1"
                     /translation="MSQDDFQRGLATRTQVMGQAFVDKAFADLDGFSRPLQEFLTEHA
                     WGSTWQREGIELKTRSLVTVGMLAALGRSTELKGHVRGALNNGASLVELREVLLHSAV
                     YAGAPAAVEAFRAAREVLNELGLSLPDDSAS"
     gene            complement(636..896)
                     /locus_tag="PKN55_13860"
     CDS             complement(636..896)
                     /locus_tag="PKN55_13860"
                     /inference="COORDINATES: protein motif:HMM:NF025619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4351 domain-containing protein"
                     /protein_id="HNX72379.1"
                     /translation="MLAERIESWFDEATRKGMQHGMLQGQRQGEAAALTRVLRRRFGE
                     LSATDQARIAQAELAELECWLDRALDASTLAAVFAADDQPAA"
     gene            complement(901..1116)
                     /locus_tag="PKN55_13865"
     CDS             complement(901..1116)
                     /locus_tag="PKN55_13865"
                     /inference="COORDINATES: protein motif:HMM:NF016629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HNX72380.1"
                     /translation="MLSPGVTSMAQYDTGYRLLFSHPEMVRDLLLGYVPAEWVAEADF
                     STLEHVNGSYVADSERQRHSDQRHHGG"
     gene            1196..2083
                     /locus_tag="PKN55_13870"
     CDS             1196..2083
                     /locus_tag="PKN55_13870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011137109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005216 - monoatomic ion channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ion transporter"
                     /protein_id="HNX72381.1"
                     /translation="MSIPLPPARAAWRNRLYQIIFEADTPAGKAFDVGLIVCILVSVA
                     LVMLDSVQDIHTRFHQELWVAEWVVTVLFSAEYLARLLSAPRPWAYARSLFGVIDLLS
                     ILPTLLMLLFPELAYLLTIRALRLLRIFRIFKLGRYIAETQIILTALRQSRQKITVFL
                     TAVCVLVVMMGSTLYVVEGPANGFTSIPKSMYWAIVTITTVGYGDIAPKTPLGQLVAS
                     LAMISGYAIIAVPTGIVSSELIRGERPRAPTRPVTCPHCLLEGHQDDARFCRACGAAL
                     LSAAPALGVADKTQRSGPG"
BASE COUNT          388 a          679 c          644 g          417 t
ORIGIN      
        1 gcgaacgcgg cgacctgttt cgcgcgctgg gccgctataa cggcagcctg ggcaaggcgg
       61 aatacccgga tctggtgctg ggcgcctggc ggcgccattg ggattactca ccgctgcctc
      121 gccgcgcgcg ccgcgccggc ccctgagggc ggattctctg gagcacacac atgagccaag
      181 acgattttca gcgtggcctg gccacccgca cgcaagtgat gggccaggct tttgtcgata
      241 aagcctttgc cgacctggac ggtttttccc gccccttgca ggaattcctc accgaacacg
      301 cctggggctc cacctggcaa cgcgaaggca tcgagctgaa aacccgcagc ctggtcaccg
      361 tcggcatgct ggccgccttg gggcgcagca ccgagctgaa aggccatgtg cgcggcgccc
      421 tcaacaatgg cgccagcctg gtggagctgc gcgaagtgct gttgcacagc gcggtgtacg
      481 ccggcgcgcc ggcagcggtc gaggcgtttc gtgccgcgcg tgaagtgctg aacgagctag
      541 ggctaagcct gccagacgac agcgccagct gagggcatgc agtacggcac gccgcacgcg
      601 ccgcgtcgga agcgtctggc aagttgcgcg ggccatcagg ccgcgggctg atcgtctgcc
      661 gcaaacaccg ccgccagcgt gctggcatcc agcgcccgat ccagccagca ctccagctcg
      721 gccaattccg cctgggcaat gcgcgcctga tccgtagcgg acaactcgcc aaaacgccga
      781 cgcagcaccc gcgtcaacgc ggcggcctca ccctgacgct gtccttgcag catgccgtgt
      841 tgcatgccct tgcgggtagc ttcgtcaaac cagctttcaa tgcgttcggc cagcatgcta
      901 tcagcctcca tgatgtcgtt gatctgaatg gcgctgccgt tcgctgtcag cgacataact
      961 gccattcaca tgctccagag tagagaaatc cgcctcggcc acccactcgg caggcacata
     1021 acccaatagc agatcgcgca ccatttccgg gtgagaaaac agcaggcggt aaccggtgtc
     1081 gtattgcgcc atggaagtca ccccgggaga gagcataaaa ttatttattg ttataagtga
     1141 tttttctgtc agccgccatg ccacaatacc tcttcctgta ctgaacgagc gccccatgtc
     1201 gatccccttg ccacccgccc gcgccgcctg gcgcaatcgt ctgtatcaga tcatttttga
     1261 agccgacacc ccggccggca aggcgtttga tgtgggcttg attgtttgca ttctggtgag
     1321 cgtggcgctg gtgatgctgg atagcgtgca ggatatccat acccgctttc accaggagtt
     1381 atgggtggcg gaatgggtgg tcactgtgct gttcagtgcg gaatacctgg cgcggctgct
     1441 cagcgcacca cggccgtggg cgtatgcgcg cagcctgttt ggggtgattg atttgctgtc
     1501 gatactgccc acactattga tgctgctgtt tcccgagctg gcctatctgc ttaccatccg
     1561 cgccttgcgg ctgttgcgga tttttcggat tttcaaactg ggacgctata tcgccgagac
     1621 acagatcatc ctcaccgccc tgcgccagag ccggcaaaaa atcaccgtat tcctgacggc
     1681 ggtgtgcgta ctggtggtga tgatgggcag cacgctgtac gtggtggaag gcccggccaa
     1741 tggctttacc agcattccca aatcgatgta ctgggcgatt gtcactatca ccacggtagg
     1801 ctacggcgac attgccccga aaaccccact gggccagttg gtcgcctcac tggcgatgat
     1861 ttccggctac gccatcattg cggtgcccac cgggattgtc agctccgagc tgattcgcgg
     1921 cgaacgcccg cgcgcaccca cccggccggt gacctgcccg cactgcctgc tggaaggcca
     1981 tcaggacgac gcgcggtttt gccgggcctg tggcgcggcc ttgttatcgg cagcgcccgc
     2041 gctgggcgtc gctgacaaaa cccagcgcag cggtcctggc taggcgtgtg accgcagaca
     2101 gtacaagtcg tacggcaagg tcacacaa
//
LOCUS       DAOPYX010000402         2115 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_77350_length_2115_cov_10.149515, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000402 DAOPYX010000000
VERSION     DAOPYX010000402.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 2115)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2115)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2115
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77350_length_2115_cov_10.149515"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..329)
                     /locus_tag="PKN55_13875"
     CDS             complement(<1..329)
                     /locus_tag="PKN55_13875"
                     /EC_number="5.4.2.2"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014087081.1"
                     /note="catalyzes the interconversion of alpha-D-mannose
                     1-phosphate to alpha-D-mannose 6-phosphate and
                     alpha-D-glucose 1-phosphate to alpha-D-glucose
                     6-phosphate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HNX72382.1"
                     /translation="MTQFPKEIFKAYDIRGIVGKTLTPEIAQAIGQAIGSEARARQVS
                     AVCIGRDGRLSGPALSNALAAGLRQAGVDVIDVGQVATPMLYFAAHQLGTLSGVMVTG
                     SHNPPEY"
     gene            complement(451..756)
                     /locus_tag="PKN55_13880"
     CDS             complement(451..756)
                     /locus_tag="PKN55_13880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX72383.1"
                     /translation="MAPDCLRGRRGKAASPGQGGGQSAARRWATLSVVLADEKRKLVG
                     NGDADLARIRQEVFGDAPASKPDEKKPEPALDLPGKQLNELADVLKKFRDLFAAGGK"
     gene            830..2089
                     /locus_tag="PKN55_13885"
     CDS             830..2089
                     /locus_tag="PKN55_13885"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNX72384.1"
                     /translation="MHDPLAIKDPESFEKHIGVLFQNAGWKVIAPPANTRGYDLELQR
                     DGHRIAVQVKNYTRKCSLADVEKFRSFLELPLADGFAYGVFVSASGFSKNALLDVEAN
                     QREVQETRQAAAAAAPSAAQPTPRPAGARGPKPVKLVEYRDGRLLGAALEAEASQPPA
                     NCRYIGVFTCKGGVGKTTTAAHLAGAFALMGHDVILLDLDPDQNLHKLFRADTDNADS
                     EASLFVPALMKNQPGATITVLSHAQWQQSQAGDYRDAKVVICDCSPVLAENPAGLIGQ
                     FDYCIIPTTLNPLGIAKNANVITRTFEHIRSLNPRAELFCLINGYDSSKAVEKKNEVL
                     LTHLQKEIDRYAQHDPKCRLIPPDEAKVRHSSALLYWGYHIVDGSRPQLAFREVAGRS
                     YPRTDFLQLAEYLENHTDLATLREPRG"
BASE COUNT          407 a          688 c          642 g          378 t
ORIGIN      
        1 ttatattccg gcgggttgtg gctgccggtc accatcacgc cagacagtgt gcccagctgg
       61 tgagccgcaa aatacagcat cggcgtggct acctggccaa cgtcgatcac gtccacgccg
      121 gcctggcgca ggccagcggc cagcgcgttg gacagggccg ggccggacaa gcggccgtcg
      181 cggccaatgc acaccgccga cacctggcgc gcgcgcgcct cgctgccaat cgcctggcca
      241 atggcctggg caatctctgg ggtgagggtt ttgccgacaa tcccacgaat atcgtaggcc
      301 ttgaagattt ccttggggaa ctgggtcatg gtggcgtctg tgcgccgcgc cgcacgcggc
      361 ggccggcgaa aataaggtga aagagtgggc tgattttacc agccaatccc actggcgtgg
      421 gcggcttgcg cgctgcgcgc tgggccaggc ttatttgccg ccagcggcaa acagatcacg
      481 aaattttttc agcacatcgg ccagctcgtt cagctgcttg ccgggcaaat ccagcgccgg
      541 ttctggtttt ttctcgtccg gcttgctggc cggggcgtcg ccgaacactt cctggcggat
      601 gcgcgccaaa tccgcatcgc cattgcccac cagcttgcgt ttttcatcgg ccagcaccac
      661 gctcagcgtg gcccagcggc gggcggcgga ttggccaccc ccctggcctg gtgaggcggc
      721 cttgccgcgc ctgccgcgca gacagtccgg ggccaattat gataaaaata tgattcatca
      781 gcagtggcgc gccgccgctg tgttttttac ccgcaaggaa acccgcccaa tgcacgaccc
      841 tttggccatc aaagacccgg aatcattcga aaaacatatc ggcgtgctgt ttcagaacgc
      901 tggctggaag gtgattgccc cgccggccaa tactcggggc tacgatctgg agctgcaacg
      961 cgacggccac cgcattgccg tgcaggtaaa aaattacacc cgcaagtgca gcctggccga
     1021 tgtggagaaa tttcgcagtt ttctggagct gccgctggcg gatgggtttg cttacggggt
     1081 gtttgtgtcg gccagcgggt tttctaaaaa cgccttgctg gatgtcgagg ccaatcagcg
     1141 cgaagtgcag gaaacccggc aagccgctgc cgccgctgcg ccgtccgccg cccagcccac
     1201 gccgcgcccg gccggcgcgc gcggccccaa gccggtgaaa ttggtggaat accgcgatgg
     1261 ccgcttgctg ggcgccgcgc tggaggccga agccagccag ccgccggcca attgccgcta
     1321 tatcggcgtg ttcacctgca agggcggcgt gggcaaaacc accaccgccg cgcatctggc
     1381 cggcgcgttt gccttgatgg gccacgatgt gatcctgctg gacctcgacc ccgaccaaaa
     1441 cctgcacaag ctgtttcgcg ccgacacgga caacgccgac agcgaagcca gcctgtttgt
     1501 gccggcgctg atgaaaaacc agcccggcgc caccatcacc gtgctcagtc acgcgcagtg
     1561 gcagcagtcc caagccgggg attatcgcga cgccaaagtg gtgatttgcg attgctcgcc
     1621 cgtgctggcg gaaaacccgg ccgggctgat tggccagttt gactactgca tcatccccac
     1681 cacgctcaac ccgctgggca tcgccaaaaa cgccaatgtg atcacccgca cgtttgagca
     1741 tatccgctcg ctcaaccccc gtgctgagct gttttgcctg atcaatggct atgattccag
     1801 caaggcggtg gaaaagaaaa acgaagtgct gctcacccat ctgcaaaaag aaatcgaccg
     1861 ctacgcccag cacgacccga agtgccggct gattccgccc gacgaagcca aagtgcgcca
     1921 cagcagcgcc ttgctgtact ggggctacca tattgtcgat ggctcacgcc cgcaactggc
     1981 cttccgcgaa gtggccgggc gcagttatcc ccgcacggat ttcttgcaac tggcggaata
     2041 cctggaaaac cacaccgact tggccaccct gcgcgagccg cgcgggtgag ggtaagcgcg
     2101 gcagcggata aactg
//
LOCUS       DAOPYX010000403         2058 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_80632_length_2058_cov_12.157763, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000403 DAOPYX010000000
VERSION     DAOPYX010000403.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 2058)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2058)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2058
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80632_length_2058_cov_12.157763"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1085)
                     /locus_tag="PKN55_13890"
     CDS             complement(<1..1085)
                     /locus_tag="PKN55_13890"
                     /inference="COORDINATES: protein motif:HMM:NF024140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MCP four helix bundle domain-containing protein"
                     /protein_id="HNX72385.1"
                     /translation="MFSNLKIGVRLAVGFAVLIALLLVVGVMASLQLRGINEDVDDVI
                     NDHTPKVAMANEMIADVNAAGIAIRNAMLIGDTQGMQSELQKVQEARARIEAGSHKLI
                     ELANADREREITKAIEENRAIYFNGIANLIRLVNAGDHKQALHLMYTDYRPLFRSYIG
                     AVNELVKYESEQVSHIGKATQQNVERTMLVVMAVSGAAVLFALLVGVTMTRSITRPLK
                     EAVDVATQLAEGKLDMHIEVKSHDETGQLLDAMRNMVTKLTQIIGEVRSAADALTNAS
                     SQVSATAQSLSQASSEQAARVEETTASIEQITASIAQNTENARVTDGMAAKAAEEAAE
                     GGEAVRRTSDAMQEIASRIGIIDDIAY"
     gene            complement(1144..>2058)
                     /locus_tag="PKN55_13895"
     CDS             complement(1144..>2058)
                     /locus_tag="PKN55_13895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014086714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HNX72386.1"
                     /translation="IGLVIRGGETELDKSLIEKVGDPLMHLVRNAMDHGIESQEKRQA
                     AGKPARGRIELNAYHDSSSVVIEVSDDGGGLPYDKILAKAVAQGLVPANQSLTDEEIA
                     ALIFRPGVSTADRVSNLSGRGVGLDVVARNIQALRGTVEIKSKPGQGSRFVIRLPLTL
                     AIIDGFLTAVGKNYFIVPLDTVVECIELPTHCDREYLNLRGEVLPFLRLRDEFELDGE
                     PSTRQSVVVVQYAGHKVGLVVDRLHGEFQTVIKPLGPLFRHLRGIGGSTILGSGEVGL
                     ILDVPLLARRASRREARSFEPAPRAHKH"
BASE COUNT          364 a          653 c          615 g          426 t
ORIGIN      
        1 tggtaggcga tgtcgtcgat gatgccgatg cgcgaggcga tttcctgcat ggcgtcgctg
       61 gtgcgacgta cggcctcgcc gccttcagcg gcttcttctg cggccttggc ggccatgccg
      121 tcggtgacgc gggcgttttc ggtgttctgg gcgatcgagg cggtgatttg ctcaatgctg
      181 gcggtggttt cttccactct ggcggcttgc tcgctggagg cttgcgacag cgactgcgcc
      241 gtggccgaca cttgcgacga ggcattggtc agcgcatcgg ctgccgagcg cacttcgccg
      301 atgatctgag tgagcttggt caccatattg cgcatggcgt ccagcaactg gccggtttca
      361 tcgtgcgatt tgacctcgat atgcatgtcc agcttgcctt cggccagttg ggtggccaca
      421 tccaccgctt ctttcagcgg gcgggtgatc gagcgggtca tggtgacgcc caccagcaag
      481 gcaaacagca cggcggcgcc agaaaccgcc atcaccacca gcatggtgcg ctccacattc
      541 tgctgggtgg ccttgccgat atgagaaacc tgttcgcttt cgtacttgac cagttcgttg
      601 accgcgccga tatagctgcg gaacaaaggc cggtagtcgg tgtacatcag gtgcagcgcc
      661 tgcttgtggt cgcctgcgtt caccagacgg atcaaattgg cgatgccatt aaagtaaatc
      721 gcccgatttt cttcgatggc cttggtgatt tcccgctcgc ggtcggcatt ggccagctcg
      781 atcagtttgt gcgaaccggc ctcaatgcgc gcgcgcgcct cttgcacctt ttgcagctcg
      841 ctttgcatgc cctgggtgtc gccaatcagc atggcgttgc ggatggcgat gccggcggcg
      901 ttgacgtcgg cgatcatttc attggccatc gccaccttgg gcgtgtggtc gttgatgacg
      961 tcgtccacgt cttcattgat gccgcgcaac tgcagtgagg ccatcacccc gaccaccagc
     1021 agcaaggcaa tcagcacggc aaaaccaacg gccagtctga ccccgatttt cagattggaa
     1081 aacacgtgta cgtccccttc aaaaaacgcc cgacgcgctg cacggcggcg cgtcggtgat
     1141 caatcaatgt ttgtgcgcgc gcggcgccgg ctcaaagctg cgcgcctcgc gtcggctggc
     1201 gcgacgcgcc agcaatggca catccagaat cagccccact tcgccgctgc ccagaatggt
     1261 cgagccgcca atgccgcgca aatggcggaa caaaggcccc aagggcttga tgaccgtctg
     1321 gaactccccg tgcagacgat ccaccaccaa gccgacctta tggccggcgt attgcaccac
     1381 caccacgctt tgccgcgtag atggctcgcc atccagctcg aactcatcgc gcagacgcag
     1441 gaatggcagc acctcgccgc gcaggttcag atattcccgg tcgcaatggg tgggcagctc
     1501 gatgcactcc accacggtgt ccagcggcac aatgaaataa ttcttgccga ctgcggtcag
     1561 gaagccgtca ataatggcca gcgtcaatgg caagcgaatg acaaagcgcg agccctggcc
     1621 cggtttgctc ttgatttcca ccgtgccgcg cagcgcctga atattgcgcg ccaccacgtc
     1681 caggcccacg ccgcgcccgg acagattgct cacccggtcg gcggtggaga cgcccggtcg
     1741 gaaaatcagc gcggcgattt cttcgtcggt caggctctgg ttggcgggca ccaggccctg
     1801 ggccacggcc ttggccagaa tcttgtcgta gggcaggccg ccgccgtcat cgctgacctc
     1861 aatcaccacg ctgctggaat catgataggc gttcagctca atgcgcccgc gcgccggctt
     1921 gccggcagcc tggcgttttt cttgtgactc aatgccatgg tccatggcgt tgcgcaccag
     1981 atgcatcagc gggtcgccga ctttttcgat cagcgatttg tccagctcgg tttcgccgcc
     2041 acgaatcacc aggccaat
//
LOCUS       DAOPYX010000404         2053 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_80950_length_2053_cov_11.204705, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000404 DAOPYX010000000
VERSION     DAOPYX010000404.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 2053)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2053)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2053
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80950_length_2053_cov_11.204705"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..263
                     /locus_tag="PKN55_13900"
     CDS             <1..263
                     /locus_tag="PKN55_13900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX72387.1"
                     /translation="QRAGRPLLCHHAPHLASGARGWPPSPDTPHEEPPTVAAAPCYFC
                     AFRTAVQFFAKSSVFCIFIVKRIFLFHVRVECITHKYGAKSA"
     gene            398..2050
                     /locus_tag="PKN55_13905"
     CDS             398..2050
                     /locus_tag="PKN55_13905"
                     /inference="COORDINATES: protein motif:HMM:NF024140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNX72388.1"
                     /translation="MKHLSVGQRLYGLVIVFSVILIAVGALGLKNAADTLAGLKTVYE
                     DRTVALSQLAQIDQRIRRNSTQFYRALALDPGSPLARHLDGSLPEYGQALRDNTRIAA
                     AEWRKYMATHLTPEEKQQAQQVDTLLTAYEKDLVIPVAEYFGRGKLDTDTSLGFIRAL
                     GKLGNDTRTALQALIDLQQRVAKQEYERAVAGNQQTRQILLALILLGLGLGGMVSWRL
                     ICSVTTPLGKIRDAVAHVQQSGDFTTHLAVQGQDEVAQTTRAFNDLLSTLRATLHSVQ
                     TATAAVSQEAEGLAGQSTQAASAAAHASESTSAMAAAVEQMTVSINHVGDASQQAIAQ
                     AEDAGQHARQGGEVIQRAVGEINQIAQSVREVSAIIEQLDGRSERISGVIQVIKEVAD
                     QTNLLALNAAIEAARAGESGRGFAVVADEVRKLAERTGKATGEISLMITEIQQSSRNA
                     VGSMDSAVQQVNAGVQLAADAGEAIVRIRNSTRQVTQTVASISESILEQGAASNTIAE
                     QVEQVARASEQNSAIARQSAASAQAVAQQAGQTRLALARFQL"
BASE COUNT          444 a          637 c          617 g          355 t
ORIGIN      
        1 gacagcgcgc ggggcgccct cttctttgcc atcacgctcc gcacttggcg agcggcgcgc
       61 gcggctggcc gccaagcccc gacacacccc atgaagagcc gcccaccgtc gcggcggcgc
      121 cgtgctattt ttgtgcgttc cgcacagcgg tgcaattttt tgccaaaagc agcgtattct
      181 gcatttttat tgttaagcgc atatttttgt ttcatgtccg tgtagaatgc atcacgcaca
      241 aatatggtgc aaaaagtgca taaaagcacc ttattggtgc aaaacaagaa gcattaacaa
      301 tatgacgtgc agcgcttcca ggtcagaatc cgctccattt ggcgcaatgt cttgcgactg
      361 tccgacttgc cgcatgcaca cttccacaga ggaatgcatg aaacatctta gcgtcggaca
      421 acgtttatat gggcttgtca ttgtattttc cgttatcttg atcgcagtgg gcgcgctggg
      481 gctgaaaaat gccgccgaca ccctggccgg cttgaaaacc gtctacgaag accgcacggt
      541 ggccttgagc caattggcgc agattgatca acgcatccgc cgcaattcta cccagtttta
      601 ccgcgccctg gcgctggacc ccggcagccc gctggccagg catctggacg gcagcctacc
      661 cgaatatggc caggcgctac gcgacaatac ccgcattgcc gctgcagaat ggcgcaaata
      721 catggccacc cacctgacgc cagaagaaaa acaacaagca caacaggtgg ataccctgct
      781 gaccgcctat gaaaaagact tggtcattcc cgtggccgag tattttggcc ggggaaaact
      841 ggacacagac acctccctgg gctttatccg tgccttgggc aagcttggca acgacacccg
      901 gacggccttg caagcgctga ttgatttgca acaacgcgtg gccaagcagg aatatgagcg
      961 cgccgtagcg ggcaatcagc aaacccggca aatcttgctg gccttgattc tgttggggct
     1021 ggggctgggc ggcatggtgt cctggcgctt gatttgcagc gtgaccacgc cgctgggcaa
     1081 aatccgcgat gcggtggcgc atgtgcagca gtctggcgat ttcaccaccc atctggccgt
     1141 gcaggggcaa gatgaagtgg cgcaaaccac ccgcgctttt aatgatctgc tgagcacctt
     1201 gcgcgccacc ctgcattcgg tgcaaaccgc cacggcggcg gtcagccagg aggccgaagg
     1261 cctggccggc caatccacgc aagccgccag cgccgccgcc cacgccagcg aatccacctc
     1321 ggccatggcc gcagcggtgg aacaaatgac ggtcagcatc aaccatgtgg gcgacgccag
     1381 ccagcaagcg atagcccagg ccgaagacgc cggacaacac gcgcgccagg gcggcgaggt
     1441 gattcagcgc gccgtgggcg aaatcaacca gattgcccag agcgtgcgag aagtctccgc
     1501 catcattgag caactggacg ggcgttccga gcgcatttca ggggtgatcc aggtcatcaa
     1561 ggaagtcgcc gaccaaacca atctgttggc gctgaacgcc gccatcgagg ccgcccgcgc
     1621 cggcgaatcc gggcgcggtt ttgccgtggt ggccgacgag gtgcgcaaac tggccgaacg
     1681 cactggcaag gccactggcg aaatcagcct gatgatcacc gaaatccagc aaagcagccg
     1741 caatgcggtg ggctcgatgg acagcgccgt gcagcaggtg aatgccgggg tgcagctggc
     1801 ggcggacgct ggcgaggcga ttgtgcgcat tcgcaacagc acccggcagg tgacgcagac
     1861 cgtggcgtcc atcagcgaat cgattctgga acaaggcgcc gccagcaaca ccattgccga
     1921 gcaggtcgag caggtggcgc gcgccagcga gcaaaacagc gccattgccc gccagtcggc
     1981 cgcctcggcc caggccgtgg cgcaacaggc cggccagacg cgcctggcgc tggcgcgttt
     2041 tcagctctga ggc
//
LOCUS       DAOPYX010000405         2035 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_82028_length_2035_cov_14.490404, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000405 DAOPYX010000000
VERSION     DAOPYX010000405.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 2035)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2035)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2035
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82028_length_2035_cov_14.490404"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..491)
                     /locus_tag="PKN55_13910"
     CDS             complement(<1..491)
                     /locus_tag="PKN55_13910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018152639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HNX72389.1"
                     /translation="MSLSQRIQFTPVDNARLQNLCGPLDENLKQIETALDVSIARRGE
                     GFKVVGARAADAVTALEMFYLRAERQPLEVEDIQLGLVEFQRTPPEAVDEGVPVLMTR
                     RGDLRGRTQRQNHYLSAIQNHDITFGIGPAGTGKTYLAVASAVDAMERDAVKRIVLVR
                     PAV"
     gene            complement(499..1839)
                     /gene="miaB"
                     /locus_tag="PKN55_13915"
     CDS             complement(499..1839)
                     /gene="miaB"
                     /locus_tag="PKN55_13915"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HNX72390.1"
                     /translation="MSKKVFIKTFGCQMNEYDSAKMLDVLGASEGAVRTDTPEDADII
                     LFNTCSVREKAQEKVFSDLGRVRELKLQRPDLVIGVGGCVASQEGEAIVKRAPFVDVV
                     FGPQTLHRLPDMLRARSDSGRAQVDISFPEIEKFDHLPPARVEGGAAFVSIMEGCSKY
                     CTFCVVPYTRGEEVSRPLDDVLVEIAGLAQQGVKEITLLGQNVNAYRGLMHDGEIADF
                     ALLLDYVHEIPGVERIRFTTSHPREFTDRIIDCYARLPKLVSHLHLPVQSGSDRVLAA
                     MKRGYTVLEYKSIIRKLRAVRPDLCLSSDFIVGFPGETEADFDATLKLAKDLAFDFSF
                     VFIYSPRPGTPAANLPDDIAHSEKVRRLEALNEVIEAEGLRINQSMVGTVQRVLVEDI
                     SRRDAEVLAARTMNNRVVNVPGLPRLLRQMIDVRITEAYPHSLRGEIVTRESIG"
     gene            complement(1958..2034)
                     /locus_tag="PKN55_13920"
     tRNA            complement(1958..2034)
                     /locus_tag="PKN55_13920"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1998..2000),aa:Met,seq:cat)
BASE COUNT          362 a          650 c          639 g          384 t
ORIGIN      
        1 tccaccgccg ggcgcaccag cacaatgcgc ttgaccgcgt cgcgctccat ggcgtccacc
       61 gcgctggcca ccgccagata ggttttgccg gtgccggccg ggccgatgcc aaaggtgatg
      121 tcgtggttct ggatggcgct cagatagtgg ttctggcgct gggtgcgccc gcgcaggtcg
      181 ccgcgccggg tcatcagcac cggcacgccc tcgtccaccg cctcgggcgg cgtgcgctga
      241 aactccacca agcccagctg gatgtcttcg acctccagcg gctggcgctc ggcgcgcagg
      301 tagaacatct ccagcgcggt caccgcgtcg gcggcgcgcg cgcccaccac cttgaagcct
      361 tcgccgcgcc gggcgatgct gacatccagc gcggtttcga tctgtttcag gttttcgtcc
      421 agcgggccgc acaggttttg caggcgggcg ttgtccaccg gggtgaattg aatgcgttgg
      481 ctcagggaca tggcgcgctc agccaatgct ttcgcgggtg acgatttctc cgcgcagcga
      541 atgggggtaa gcctcggtga tgcgcacgtc gatcatctgg cgcaacaggc gcggcaggcc
      601 gggcacgttc accacccggt tgttcatggt gcgcgccgcc agcacctcgg cgtcgcggcg
      661 cgagatgtct tccaccagca cgcgctgcac cgtgcccacc atgctttgat tgatgcgcag
      721 gccctcggct tcgatcacct cgttcagcgc ctccagccgg cgcaccttct cgctgtgcgc
      781 aatgtcatcc ggcagattgg ccgccggcgt gccggggcgc gggctgtaga tgaacacaaa
      841 gctgaaatca aaagccaggt ccttggccag cttcagcgtg gcgtcaaagt ccgcctcggt
      901 ttcgccgggg aagccgacaa taaaatccga gctcaggcac aagtccgggc gcaccgcgcg
      961 cagcttgcgg atgatggatt tatattccag cacggtgtag ccgcgcttca tcgccgccag
     1021 cacccgatcc gagccggatt gcaccggcag atgcagatgc gacaccagct tgggcaggcg
     1081 cgcgtagcag tcaatgatgc ggtcggtgaa ttcgcgcgga tggctggtgg tgaagcggat
     1141 gcgctccacg ccgggaatct cgtgcacata gtccagcagc agggcaaagt cggcgatttc
     1201 accgtcgtgc atcaggccgc gataggcgtt gacgttctgg cccagcaggg tgatttcttt
     1261 taccccctgt tgcgccaggc cggcgatttc caccagcaca tcgtccagcg gacgcgagac
     1321 ttcttcgcca cgggtatagg gcaccacgca gaaggtgcag tatttcgagc agccttccat
     1381 gatggagaca aacgccgcgc cgccttccac ccgcgctggc ggcagatggt cgaatttttc
     1441 gatttccggg aaggaaatat ccacctgggc gcggccgctg tcgctgcgcg cgcgcagcat
     1501 gtccggcagg cggtgcaggg tttgcgggcc aaacaccaca tcgacaaagg gcgcgcgttt
     1561 gacaattgcc tcgccctcct gactggccac gcaaccgccc acgccaatca ccagatcagg
     1621 gcgttgcagc ttgagctcgc gcacgcggcc caagtcggaa aacacttttt cctgggcttt
     1681 ttcgcgcacc gaacaggtgt tgaacagaat gatgtcggcg tcttccgggg tgtcggtgcg
     1741 gacagcgcct tcgctggccc ccagcacatc cagcattttg gccgagtcat actcgttcat
     1801 ctggcagccg aaggttttga taaagacttt cttgctcacg ggaatccgtc tgggtgacag
     1861 ggtggctaaa cagcccgcca tgatacccca agccatccgg cggcgggcgg gcgacaatgc
     1921 aaaaagcccg tcgctgaggc gacgggcttg gaatctttgg tggctatgac tggagttgaa
     1981 ccagtgaccc cagcattatg agtgctgtgc tctaaccaac tgagctacat agcca
//
LOCUS       DAOPYX010000406         2023 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_82763_length_2023_cov_14.309959, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000406 DAOPYX010000000
VERSION     DAOPYX010000406.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 2023)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2023)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2023
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82763_length_2023_cov_14.309959"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..923)
                     /locus_tag="PKN55_13925"
     CDS             complement(<1..923)
                     /locus_tag="PKN55_13925"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014088523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNX72391.1"
                     /translation="MTRLAIVRQRYNPAGGAERFVSRALDALARQGSLDVTLITRRWE
                     DHAGYHSLTVNPFYLGNVWRDAGFARGVRQAIAREGFDLVQAHERIPGCDVFRAGDGV
                     HRAWLARRGRALSALGRLKLKLNPYHHYICRAEARMFADPALKAVICNSRMVADEIQQ
                     YFGLPADKLAVIYNGVDTQAFHPGLASQRDAVRAHWRIPAQAPLLVYVGSGFERKGVA
                     AALAAIAPLAGVHLLVVGHDKHAARYQAQARALGVAERVIFTGAQKDVKSFYAAADAL
                     ILPTLYDPFPNVCVEALACGLPLFTSTGCGAA"
     gene            complement(920..2005)
                     /gene="rfaQ"
                     /locus_tag="PKN55_13930"
     CDS             complement(920..2005)
                     /gene="rfaQ"
                     /locus_tag="PKN55_13930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018748353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipopolysaccharide heptosyltransferase
                     III"
                     /protein_id="HNX72392.1"
                     /translation="MLQDAIDPAALKRVLVIKLRHHGDVLLTSPVFTTLKRCAPQAEL
                     DALVYADTAPMLRGHPALSQLFTIDRHWKRAGRWAQLRHEWALFQALRARRYDLIVHL
                     TDHNRGATLARLLKPRWAVAPAGKTSRRFAKAFTHRYPVVGGNRRHTVELHLDALRRL
                     GLHASAAERGLVLCPSAEARQRVDAKLAALGLPAGQFIHIHPTSRWLFKTWPTTHMAA
                     LIDALTARGWPVLLTAAPDPAELAMLADIQGRLARPVASLAGELTLEELGAALQGARA
                     FVGMDSVPMHMAAAVGTPTVALFGPSGDIEWGPWRTPHRVVREDLPCRPCGRDGCGGG
                     KLSECLSLIAPARVLAALDELLQETGR"
BASE COUNT          318 a          677 c          741 g          287 t
ORIGIN      
        1 gccgcgccgc agccggtgct ggtgaacagc ggcaggccgc aggccagcgc ttcgacgcag
       61 acattgggga aggggtcgta cagggtgggc aggatcagcg cgtcagcggc ggcgtaaaag
      121 cttttgacgt ctttttgcgc gccggtaaag atcacccgct cggccacgcc cagcgcgcgc
      181 gcttgcgcct ggtagcgggc ggcgtgtttg tcgtggccca ccaccagcag atgcacgccg
      241 gccagcgggg cgatggcggc cagcgcggcg gccacgcctt tgcgctcaaa gcccgagccg
      301 acatacacca gcagcggcgc ttgcgcgggg atgcgccagt gggcgcgcac ggcgtcgcgc
      361 tggctggcca ggccggggtg aaacgcctgg gtgtccacgc cgttgtaaat caccgccagc
      421 ttatcggccg gcaggccgaa atactgctgg atttcatcgg ccaccatgcg cgagttgcag
      481 atcaccgctt tgagcgccgg atcggcgaac atgcgcgctt cggcgcggca gatgtaatgg
      541 tgataggggt tgagcttgag tttcagccgg cccagcgccg acagcgcgcg gccgcgtcgg
      601 gccagccagg cgcgatgcac gccgtcgcca gcgcgaaaca catcgcagcc ggggatgcgc
      661 tcgtgcgcct gcaccaggtc aaagccttcg cgggcgatgg cctggcgcac gccacgggca
      721 aagccggcgt cgcgccagac attgcccaga taaaacgggt tgacggtcag gctgtggtag
      781 ccggcgtggt cttcccagcg gcgggtgatc aaggtcacgt ccaggctgcc ctggcgggcc
      841 agggcgtcca gcgcgcggct gacaaagcgc tcggcgccgc cagcggggtt atagcgttgc
      901 cgcacaatcg ccaggcgggt catcggccag tctcctgcaa gagttcatcc agcgccgcca
      961 gcacgcgcgc cggggcaatc aggctcaggc attcgctgag cttgccgccg ccgcagccat
     1021 cgcgcccgca cggccgacag ggcaggtctt cgcgcaccac ccgatgcggc gtgcgccacg
     1081 gcccccattc gatgtcgcca gacgggccaa acagcgccac ggtgggcgtg cccaccgccg
     1141 ccgccatatg catcggcacc gaatccatgc caacaaaggc gcgcgcgcct tgcagcgcgg
     1201 cgccgagttc ttccagcgtc agttcgccgg ccaggctggc caccggccgc gccagccggc
     1261 cttgaatatc agccagcatg gccagttcgg ccgggtcggg cgcggcggtg agcagcaccg
     1321 gccagccgcg cgcggtcagc gcgtcgatca gcgccgccat atgcgtggtg ggccaggttt
     1381 tgaacagcca gcgcgaggtg gggtgaatat ggatgaactg cccggccggc aggcccagcg
     1441 ccgccagttt ggcgtccacc cgctggcgcg cctcggccga tgggcacagc accagcccgc
     1501 gctctgccgc gctggcgtgc aggcccaggc ggcgcagggc gtccaggtgc agctcgacgg
     1561 tgtgccggcg gttgccgccc accaccgggt agcggtgggt gaaggccttg gcgaagcgcc
     1621 ggctggtttt gccggccggg gccaccgccc agcgcggctt gagcaggcgc gccagggtgg
     1681 cgccgcgatt gtggtcggtg aggtggacga tcaaatcata acggcgcgcg cgcagggcct
     1741 gaaacagcgc ccattcgtga cgcagctgcg cccagcggcc ggcgcgcttc cagtggcggt
     1801 cgatggtgaa cagttggctc agcgccgggt ggccgcgcag catcggcgcg gtgtcggcgt
     1861 acaccagcgc atccagctcg gcctgtgggg cgcaacgctt gagggtggtg aacaccgggg
     1921 aggtgagcag cacatcgccg tgatggcgca gtttgatcac cagcacgcgc ttgagcgcgg
     1981 cggggtcgat ggcgtcttgc agcatgaaaa cgatagggcc tgt
//
LOCUS       DAOPYX010000407         2023 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_82812_length_2023_cov_1.887703, whole genome shotgun sequence.
ACCESSION   DAOPYX010000407 DAOPYX010000000
VERSION     DAOPYX010000407.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 2023)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2023)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2023
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82812_length_2023_cov_1.887703"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>887)
                     /locus_tag="PKN55_13935"
     CDS             complement(<1..>887)
                     /locus_tag="PKN55_13935"
                     /inference="COORDINATES: protein motif:HMM:NF012493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HNX72393.1"
                     /translation="YPQYGTKVCKVIHNAIPHVDTLIKEFKKPDNDFRIAISVDMLDT
                     GIDVPEVVNLVFAKPVKSKVKFWQMIGRGTRLCPDLFGKGEDKKEFLIFDHWNNFAYH
                     EMEPQEAEPKAPKSLAQRRYEARLALAHAALRGHEPAAFASVCELLLADARALNDQTI
                     AVRDHWPAVQLARDAANVNAFAPATAQMLADTIAPLLNALDLRGQGDALRWDILLANA
                     QHEAIAKPDQANACRADILAWLARLPPNLNAVRAKADELKAIQSDSYWHHPDFARLEA
                     SRLALRELMHLAERPTLPP"
     assembly_gap    888..897
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<898..1746)
                     /locus_tag="PKN55_13940"
     CDS             complement(<898..1746)
                     /locus_tag="PKN55_13940"
                     /inference="COORDINATES: protein motif:HMM:NF037510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HNX72394.1"
                     /translation="MQATGTGKTRVAIALSKLMIEAKWAKRILFLCDRKELRKQAKKV
                     FNDYLKEPIYVFGQQNEVARQDARIYIGIYQGLINQYENFDVGFFDLIIADESHRSIY
                     NQYGDIFSYFDALQVGLTATPVEMVSRSTCRLFGCDYKTPTANYTLEDAVRDGNLAPF
                     RVVTHTTKFMREGIKGHDLSDEQIAQLEDQGIDPNTLEFDGEDINQAVYNKDTNRVIL
                     RNLMDKGIRDADGQLPGKSIIFARNIRHARLLAELFDELYPQLAGKFCAVIHSDESRA
                     EQLIDDF"
     gene            1741..1935
                     /locus_tag="PKN55_13945"
     CDS             1741..1935
                     /locus_tag="PKN55_13945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX72395.1"
                     /translation="MHDQRLAMLAGKAFFDAVKGYVIKFKYDFVTTEKFKEYWHSMAD
                     VGEIIDAYLSVPAQSAPNAE"
BASE COUNT          381 a          528 c          650 g          454 t
ORIGIN      
        1 gggcggcagg gtcgggcgct cggccaggtg catcagctcg cgcagcgcca ggcggctggc
       61 ttccagccgg gcaaagtcgg ggtgatgcca atagctgtcg ctctgaatgg ccttgagttc
      121 gtcggctttg gcccgtacgg cgttgaggtt gggcggcagg cgcgccagcc aggcgaggat
      181 gtcggcccgg caggcgttgg cttggtctgg cttggcaatg gcttcgtgct gggcattggc
      241 cagcaggatg tcccagcgca gggcatcgcc ctggccgcgc aggtccagcg cgttgagcaa
      301 gggggcgatg gtgtcagcca gcatttgggc ggtggccggc gcaaaggcgt tgacattggc
      361 cgcatcgcgc gccagttgca cggcgggcca gtggtctcgc acggcaatgg tctggtcgtt
      421 cagcgcgcgg gcatccgcca gcagcagctc gcacacgctg gcaaaggcgg ctggctcgtg
      481 gccgcgcagg gcggcgtgcg ccagcgccag ccgggcttcg taacgacgct gggccagcga
      541 tttgggcgct ttgggttcgg cttcttgtgg ctccatttcg tggtaggcaa agttgttcca
      601 gtggtcgaag atcaaaaatt cttttttatc ttcgcctttg ccaaacaggt caggacacag
      661 gcgtgtgcca cggccgatca tctgccagaa cttgaccttg gatttcactg gcttggcaaa
      721 caccaggttg accacttcgg gcacgtcgat gccggtgtcg agcatgtcca ccgagatggc
      781 gatgcggaag tcgttatccg gctttttgaa ctctttgatc agggtatcca cgtgcggaat
      841 agcattgtgg atgaccttgc agaccttggt gccgtactgc gggtaaannn nnnnnnngaa
      901 gtcgtcgatc aattgctcgg cgcgcgattc gtcagagtgg atcaccgcac agaacttgcc
      961 ggccaattgc ggatacagct cgtcgaacag ctcggccagc aggcgggcat ggcggatatt
     1021 gcgggcaaag atgatgcttt tgcccggcag ctggccatcg gcgtcacgaa tgcccttgtc
     1081 catcaggttg cgcaggatga cgcggttggt gtctttgttg tacaccgcct ggttaatgtc
     1141 ttcgccgtcg aattccagcg tattggggtc gatgccctgg tcttcaagct gggcaatctg
     1201 ctcgtcgctc aggtcatggc ccttgatgcc ctcgcgcatg aatttggtgg tgtgggtgac
     1261 cacacggaac ggcgccagat tgccatcacg cacggcgtct tccagcgtgt agttggcggt
     1321 gggggttttg tagtcgcacc caaacaggcg gcaggtgctg cggctgacca tctccaccgg
     1381 cgtggcggtg aggccaactt gcaaggcgtc aaagtagctg aagatatcgc cgtactggtt
     1441 gtaaatcgag cggtgcgatt cgtcggcaat gatcagatca aagaaaccca cgtcaaagtt
     1501 ttcgtactgg ttgatcagcc cctggtaaat gccgatgtag atgcgggcat cctgcctggc
     1561 gacttcattt tgctggccaa agacataaat gggctctttc aggtagtcgt tgaacacctt
     1621 tttggcctgt ttgcgcagct ctttgcggtc gcacaaaaac agaatgcgct tggcccactt
     1681 ggcttcgatc atcaatttgg acagcgcaat ggcgacgcgg gttttgccgg tgccggtggc
     1741 ttgcacgatc agcgccttgc gatgcttgct ggcaaagcgt tctttgatgc ggtaaaaggc
     1801 tatgtcataa agtttaagta tgattttgtg acaactgaaa agtttaaaga gtattggcac
     1861 agcatggcgg atgtcggcga aatcatcgat gcatatcttt ccgtgcctgc gcagagcgcg
     1921 ccgaatgcgg aataaaggac acgaacgtcc gaacagtgaa cgatatgcag ccggaagctg
     1981 cgcagcgaac ccgtacccgc ttgaatcaaa tcgagtgggt tta
//
LOCUS       DAOPYX010000408         2020 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_82942_length_2020_cov_11.697710, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000408 DAOPYX010000000
VERSION     DAOPYX010000408.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 2020)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2020)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2020
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82942_length_2020_cov_11.697710"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(17..1210)
                     /gene="hemW"
                     /locus_tag="PKN55_13950"
     CDS             complement(17..1210)
                     /gene="hemW"
                     /locus_tag="PKN55_13950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HNX72396.1"
                     /translation="MIPLQFHPRAAAWRLDSLPPLSLYIHFPWCVRKCPYCDFNSHEP
                     RSALDQDGYIDALIADLESALPLIWGRRVQTVFMGGGTPSLFSPAHIDRLLRALDARL
                     KLNPDAEITLEANPGTLEIAQFAGYRAAGVNRLSIGVQSFDPGHLSALGRIHGADEAR
                     RAAEQASRHFDTFNLDIMYALPGQTLEQALADVEQALALGARHLSAYHLTLEPNTLFA
                     ARPPANLPDDDLAADMQEAIEARLAAAGFVHYETSAFALPGHQARHNLNYWTFGDYLG
                     IGAGAHGKLSFHDRIQRDMRHKQPAAYLAGVAQGSQVQTSQPVAHADLAFEFMMNALR
                     LTEGFALSLFNERTGLPLSAILPALEQAQTRGWVQRDGDWLRPTLAGQRFLNDLLGLF
                     LPDQD"
     gene            complement(1207..1806)
                     /gene="rdgB"
                     /locus_tag="PKN55_13955"
     CDS             complement(1207..1806)
                     /gene="rdgB"
                     /locus_tag="PKN55_13955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018747206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HNX72397.1"
                     /translation="MLKKLVLASNNAGKLREFSALLAPLGCQVIAQGELNVPEAEEPF
                     HTFIENALAKARHASRLTGLPALADDSGLCVDALGGAPGVLSARFAGEPKSDARNNAA
                     LLAALDGKTPRSGHYYCVLVLVRHADDPQPIIADGVWRGQVLSAAQGDGGFGYDPLFQ
                     AQGESVSVAEMDAAAKNAVSHRGQAMRALAAKLAELGQA"
BASE COUNT          312 a          640 c          723 g          345 t
ORIGIN      
        1 gtgcatctgg ccgccgtcag tcttgatccg gcaaaaacag ccccagcaaa tcattcaaaa
       61 agcgctgccc ggccaaggtc gggcgcagcc agtcgccgtc gcgctgcacc cagccgcgcg
      121 tctgcgcttg ctccagcgcc ggcaggatgg cgctgagcgg caggccggtg cgttcgttga
      181 acaggctcag cgcaaagccc tcggtcaggc gcagcgcgtt catcatgaac tcaaacgcca
      241 ggtcagcgtg cgccaccggc tggctggtct gcacctggct gccctgcgcc acgccggcca
      301 gataggctgc cggctgtttg tggcgcatat cgcgctggat gcggtcgtga aacgacagct
      361 tgccatgcgc gccggcgcca atgcccagat aatcgccaaa cgtccagtaa ttcaggttgt
      421 gccgcgcctg atggccgggc agggcaaacg ccgaggtttc gtaatggaca aatccagccg
      481 ccgccagccg cgcctcgatg gcctcctgca tatcagccgc caggtcgtcg tcgggcagat
      541 tggccggcgg gcgggcggca aacagggtgt tgggctccag cgtcaggtga taggccgaca
      601 gatggcgcgc gcccagcgcc agcgcctgtt cgacatcggc cagcgcctgc tccagcgtct
      661 ggccgggcag ggcgtacatg atgtccagat tgaaggtgtc gaagtggcgg ctggcctgct
      721 cggcggcgcg gcgcgcttcg tcggcgccat gaatgcgccc cagcgcgctc aggtggccgg
      781 ggtcaaagct ttgcacgcca atcgacagcc ggtttacccc cgccgcgcga tagccggcga
      841 actgggcgat ttccagcgtg ccggggttgg cttccagggt gatttctgcg tccgggttga
      901 gcttgagccg ggcgtccagc gcgcgcagca ggcggtcgat atgcgccggg ctgaacaggc
      961 tgggcgtgcc gccgcccatg aacaccgttt gcacccgccg gccccagatc aagggcaggg
     1021 cgctttccag gtcggcaatc agcgcgtcga tatagccgtc ctggtccagc gcgctgcgcg
     1081 gctcgtgcga gttgaagtcg caatacgggc atttgcgcac gcaccaggga aagtggatgt
     1141 acagcgacaa gggcggcagg ctgtccagcc gccaggcggc ggcgcgcggg tgaaattgca
     1201 gcggaatcat gcctgcccca gctcggccag cttggccgcc agcgcgcgca tggcctggcc
     1261 gcgatggctg acggcgtttt tggcggcggc gtccatctca gccactgaca cgctttcacc
     1321 ctgcgcctgg aacagcgggt cgtagccaaa gccgccgtcg ccttgcgcgg cgctgagcac
     1381 ctggccgcgc cagacgccgt cggcgatgat gggttgtggg tcgtcggcgt ggcgcaccag
     1441 caccagcacg cagtagtagt ggccgctgcg cggggttttc ccgtccagcg cggccagcag
     1501 ggcggcgttg ttgcgggcgt cggatttggg ttcgccggca aagcgtgcgg acagcacgcc
     1561 gggggcgccg cccagggcgt cgacgcacag gccggagtcg tcggccagcg ccggcaggcc
     1621 ggtgagccgg ctggcgtggc gggctttggc cagggcgttt tcgataaagg tgtggaaggg
     1681 ttcttcggct tccgggacgt tcagttcgcc ttgggcgatg acttggcagc ccaggggggc
     1741 gagcagggcg gagaattcgc gcagtttgcc ggcgttattg ctggccagca ccagtttttt
     1801 gagcatgtgg gaatccttat acaaggcgcc gcgctgtgcg cggggtttga tttggggcgg
     1861 ggtttcaccc cgcgcccgac cctatttctt tgtctcgcca aagaaatagg ggaaagaaag
     1921 gcgacccggg tgtcgcggtc agccctgcgg gctgactgcc ttgcgcttct cgaaagtcag
     1981 ggccggcccg gaatggcgcg ccgcctgcgg cgtcgcttag
//
LOCUS       DAOPYX010000409         2015 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_83269_length_2015_cov_8.213776, whole genome shotgun sequence.
ACCESSION   DAOPYX010000409 DAOPYX010000000
VERSION     DAOPYX010000409.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 2015)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2015)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2015
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83269_length_2015_cov_8.213776"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..390
                     /locus_tag="PKN55_13960"
     CDS             <1..390
                     /locus_tag="PKN55_13960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014086125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNX72398.1"
                     /translation="LGLENRLTDRIGLLSGGQRQAVSLLMASLKPSRMLLLDEHTAAL
                     DPKTAAFVLELTDQVVAEGKLTTLMVTHSMRQALDHGDRTVMLHQGRVVLDVSGEERA
                     RMDVPDLLAMFERTRGEAITDDALLLG"
     gene            546..1178
                     /locus_tag="PKN55_13965"
     CDS             546..1178
                     /locus_tag="PKN55_13965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014087462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNX72399.1"
                     /translation="MKTYDRIVAASLALFNEQGERAVTTNHIAAHLGISPGNLYYHFR
                     NKEDIVHQIFLEYQQFVRQRLTLADGRQPEVADVLGYLDTAFDGMWRYRFMFYDLPGL
                     LARNPALQHDYQRFIDDELTDTLHGLFVRCADAGLFAIDAADIRPLVINIWLVVKFWY
                     AFEQASHPSQPISEATGRRGVRQVLALLKPYVTATLRPAFIAACARYGEA"
     gene            1444..>2015
                     /locus_tag="PKN55_13970"
     CDS             1444..>2015
                     /locus_tag="PKN55_13970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HNX72400.1"
                     /translation="MFDWLAEPSPPPHVGLLLLPGFSLAELALWRTAHACLQQLTGPA
                     AGTCPTLSVDGQPVLSDLGLPVPADGALTPAALDTLSVLLVFASGLPAALPPSDALVV
                     TLSAAIHPRRVLGGVHAGAFWLAAAGLLSGCRACVHWSLHEAFAERYPQVALSANLFE
                     FDQQRLTCGGGVAVLDLFLALTARQHGQALA"
BASE COUNT          318 a          691 c          619 g          387 t
ORIGIN      
        1 ctgggcctgg aaaaccgcct gaccgaccgc attggcctgc tgtcgggcgg ccagcgccag
       61 gcggtcagcc tgctgatggc ctcgctcaag ccctcgcgca tgctgctgct ggacgaacac
      121 accgccgcgc tcgacccgaa aaccgccgcc tttgtgctgg aactgaccga ccaagtggtg
      181 gcggaaggca agctgaccac gctgatggtg acccactcga tgcgccaggc gctggaccat
      241 ggcgaccgca ccgtgatgct gcatcagggc cgggtggtgc tggacgtcag cggcgaagag
      301 cgcgcgcgca tggacgtgcc ggacttattg gccatgttcg agcgcacgcg cggcgaggcg
      361 attaccgacg acgccttgct gctggggtaa gcgccgcgca caaagcccgc ctggcgtggt
      421 tcactgcgtg ttacacgcct gatctctccc tgcaccggcc agctgccact tggcagccac
      481 tggccggtgc agggatactg cccgccttcc cgtcttgcct ggccgtgcgg ccagcctgct
      541 gaatcatgaa aacctacgat cgcatcgtgg ccgccagcct ggccttgttc aatgagcaag
      601 gcgagcgcgc ggtcaccacc aaccacattg ccgcccacct gggcatcagc cccggcaatc
      661 tgtactatca ctttcgcaat aaagaagaca tcgtccacca gatttttctg gaatatcagc
      721 aatttgtccg ccagcgcctc accctggccg acggccgcca gccggaagtg gccgatgtgc
      781 tgggctatct ggatacagcg tttgacggca tgtggcgtta tcgcttcatg ttttacgacc
      841 tgcccggcct gctggcgcgc aatccggcgc tgcaacacga ttatcagcgc tttattgacg
      901 acgaactcac cgacaccctg cacggcctgt ttgtgcgctg cgccgatgcc ggcctgtttg
      961 ccatcgacgc cgccgacatc cgcccgctgg tgatcaatat ctggctggtg gtcaagttct
     1021 ggtacgcctt cgaacaagcc tcacacccca gccagcccat cagcgaagcc accggtcgcc
     1081 gtggcgtgcg ccaggtgctg gccctgctca aaccctacgt caccgccacc ctgcgcccgg
     1141 cctttatcgc cgcctgcgcc cgctacggcg aagcctgagc cgcgcgggca tcgcctcttt
     1201 ctgtgttcgc gggcttgccg cgccacctgc acaccgatac cacaacacag cccgggctca
     1261 gctttcatca acttttggtc gcatccacgc aaggcgcggg ctgcgtggca tgatggaata
     1321 cccgctgagc cggcgctgtt gcatgccacg gcatgtctgc cggcagacca atgaacacac
     1381 cgcgttgacc cgatgtgcag ataccacctc tgacctgccg cctgagtgag tggatgaacc
     1441 accatgtttg actggcttgc cgaacccagc ccaccaccgc acgtgggcct attgttattg
     1501 cctgggtttt cgctggccga gctggccttg tggcgcacgg cgcatgcttg cctgcagcaa
     1561 ctgaccgggc cagccgccgg cacttgtcca acgctgtcgg tggacggcca gccagtgctg
     1621 tccgatctgg gcctacccgt ccccgcagac ggcgcgctga cgcctgcggc gctggacacg
     1681 ctgtcggtgc tgctggtgtt tgccagcggc ctgccagcag cgctgccgcc cagcgatgcg
     1741 ctggtggtca cgctgtcggc cgcgatacat ccgcggcggg tgctgggcgg ggtgcatgcc
     1801 ggggcttttt ggctggccgc cgccgggcta ttgagcggtt gccgcgcttg cgtgcattgg
     1861 tctttgcatg aggcctttgc cgaacgctat ccgcaagtgg cgctgtcggc taatttattt
     1921 gaatttgacc agcagcggct gacctgcggt ggcggcgtgg cggtgctgga cctgtttctg
     1981 gcgctcaccg cccgccagca cggccaggcg ctggc
//
LOCUS       DAOPYX010000410         1996 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_84461_length_1996_cov_8.674910, whole genome shotgun sequence.
ACCESSION   DAOPYX010000410 DAOPYX010000000
VERSION     DAOPYX010000410.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 1996)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1996)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1996
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84461_length_1996_cov_8.674910"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1996)
                     /locus_tag="PKN55_13975"
     CDS             complement(<1..>1996)
                     /locus_tag="PKN55_13975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012696185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNX72401.1"
                     /translation="FTGIAETRVNILQQWAQEQWQHLDALADQLAAGMPQVDGAILRF
                     RHQLADAFSELCVIDVHGVVLASTASNREGRQDLPAAAVARGVQQRFLHGPYVDPVTL
                     ALGPSTSKFHDAVTLMFYQPLLQNGQPVGAVCGRVPNDVVSDLIQREAGHIFHESGDN
                     YLFMAKSAFDPNIAPGTALSRSRFEDKTFSLGDNLKDGIRTAWGVVRVQQHTEFELVF
                     NDPATGQLHPGVRETIRCGENLFVTYPGYSDYRHIPVIGRGVTFQLPGSPDTWGMMCE
                     ADLEEVYRHRTISYRLTRLYITTMLITWAAASGMDAVFDLDLHSRSALALMAHLFGAW
                     LFLTFGTRKLTNRFRQMIRMVRTIAEGGGDLRQRLDRSTGRTDELTVMAQWFNSFIDN
                     LEGIVRRVIHTSNELSTTNQVMQRGHQDTRDTTHSVLGLMDDMFASLAGQMREIDAAN
                     LSAVEMKRLMQEMMEAASQQVSLVRQRSQGIRDSVAESSRTLTQLETSTVEIGRVVGV
                     ISEIADQTNLLALNAAIEAARAGEQGRGFAVVADEVRKLAERTSKATGEIRGMIEGVQ
                     TQAHGAVQSMGVGMREMEEGLKLAEETASDNGEVHRLVEQLFHAIDGIAATSRAHNDK
                     VKQVSDATQSTSQVLAETGHTTQQTLFASNRLQQLVNQFKV"
BASE COUNT          334 a          595 c          661 g          406 t
ORIGIN      
        1 accttgaact gattgaccag ttgttgcagg cggttggagg caaacagcgt ttgctgggtg
       61 gtgtggccgg tttcggccag cacctggctg gtgctttgcg tggcgtcgct gacttgtttg
      121 accttgtcgt tgtgcgcgcg gctggtggcg gcgatgccgt cgatggcgtg gaacaattgc
      181 tcgaccagcc gatgcacttc gccgttgtcc gaggcggttt cttcggccag tttcaggcct
      241 tcttccattt cgcgcatgcc cacccccatg ctttgcacgg cgccgtgggc ctgggtttgc
      301 acgccttcga tcatgccgcg aatttcgccg gtggccttgc tggtgcgttc ggccagcttg
      361 cgcacctcat cggccaccac ggcaaagccg cgcccttgtt cacccgcgcg ggcggcttca
      421 atcgctgcat tcagcgccaa gagattggtc tggtcggcga tttcgctgat cacgcccacc
      481 acgcggccga tttctaccgt gctggtttcc agctgggtca gcgtgcggct ggattcggcc
      541 accgagtcgc ggatgccttg cgagcgctgg cgcaccagcg acacttgctg gctggcggct
      601 tccatcatct cttgcatcag ccgcttcatt tccacggcgg acagattggc ggcgtcgatt
      661 tcgcgcatct ggccggccag cgaggcgaac atatcgtcca tcagacccag cactgaatgg
      721 gtggtgtcgc gggtgtcctg gtggccgcgc tgcatcacct ggttggtggt ggacagctcg
      781 ttggaggtgt ggatcacccg gcgcacgatg ccttcgaggt tgtcgataaa gctgttgaac
      841 cattgcgcca tgacggtgag ttcgtcggtg cggccggtgc tgcggtccag ccgctggcgc
      901 aaatcgcccc cgccttcggc aatggtgcgc accatgcgga tcatctggcg gaagcggttg
      961 gtcagcttgc gggtgccaaa ggtgagaaac agccacgcac caaacaagtg cgccatcagt
     1021 gccaatgcac tgcgtgagtg caaatccaga tcaaataccg catccatgcc actggcggct
     1081 gcccaggtaa tgagcatggt ggtgatataa agccgggtca gccggtaact gatggtgcgg
     1141 tggcggtaga cttcttccag gtcggcttcg cacatcatgc cccaggtgtc gggcgagccc
     1201 ggcagctgga aggtgacccc ccggccgatg accggaatat gccggtagtc ggaatagccg
     1261 gggtaggtga caaacaggtt ctcgccacag cggatggttt cgcgcacgcc agggtgcagc
     1321 tggccggtgg ccgggtcgtt gaacaccagt tcaaattcag tgtgttgctg cacgcgcacc
     1381 acgccccagg cggtgcgaat gccgtccttg aggttgtcgc ccagcgagaa ggttttgtct
     1441 tcaaagcgcg agcgggacaa ggcggtgccg ggggcgatgt tgggatcaaa cgccgacttg
     1501 gccatgaaca ggtagttgtc accggattca tgaaagatat gcccggcttc gcgctggatc
     1561 aggtcgctga ccacgtcgtt gggcacgcgg ccgcacacgg cgcccaccgg ctggccgttt
     1621 tgcagcagcg gctgataaaa catcagggtg acggcgtcgt gaaatttgga cgtgctcggg
     1681 cccagcgcca gcgtcaccgg atcgacatac gggccgtgca aaaagcgctg ctgcacgccg
     1741 cgcgccacgg cggcggccgg caagtcctgg cggccttcgc ggttgctggc ggtggaagcc
     1801 agcaccacgc cgtggacatc aatgacgcac agctcagaaa aagcgtcggc cagttggtgg
     1861 cgaaaacgca ggatggcccc atccacctgc ggcatgcctg cggccagttg gtcggccagc
     1921 gcgtccaggt gctgccattg ctcctgcgcc cattgttgca ggatgttcac ccgggtttcg
     1981 gcaatgccgg tgaatg
//
LOCUS       DAOPYX010000411         1993 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_84669_length_1993_cov_11.436017, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000411 DAOPYX010000000
VERSION     DAOPYX010000411.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 1993)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1993)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1993
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84669_length_1993_cov_11.436017"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            14..1846
                     /locus_tag="PKN55_13980"
     CDS             14..1846
                     /locus_tag="PKN55_13980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014085533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNX72402.1"
                     /translation="MFSWFERRINPYPDGPPPQPPAGFFPFLWAASAGVRGLIVAMTL
                     LTATIGVFEALLFSMLGSVVDWLSQTPASQLWAQQKGHLLLLAGILLASPLLVTVQSL
                     CKFQGLQGNFPMRLRWNFHRHLLGQSMRFYQDEFAGRVSAKVMQTALAVRDTMMIVGD
                     IMVFITIYFVTMVAVVGGFDLALMTPFLGWLAGYVLALKIFIPRLSRAASAQADARSL
                     MTGRITDAYTNIATVKLFSQGEREAGFARAAMQDFMRTVYRQMRLASSFEIVNHTLSM
                     LLIACTAGAALWRWSEGEVSIGAVAAATAMALRLNGISHWIMWEMSNLFEHIGTVQDG
                     ISTLSTPVRVTDRADASALQVTRGEIRFEQVSFSYGGDKNVIDQLDLTIRPGEKIGLV
                     GRSGAGKSTLVNLLLRFDDVGAGRILIDGQDIARVSQDSLRSQVGMVTQDTSLLHRSV
                     RDNLCYGRPEASDAEMVAAARRAEAHEFILGLGDPAGRRGYEAHVGERGVKLSGGQRQ
                     RIAIARVMLKNAPILLLDEATSALDSEVEAAIQGSLYQLMEGKTVVAIAHRLSTIAAM
                     DRLIVLDQGRIVEQGDHASLLAQGGLYARLWAHQSGGFLAEGAE"
BASE COUNT          329 a          591 c          674 g          399 t
ORIGIN      
        1 ttgaaggtgt tacatgtttt cctggtttga gcgtcgcatc aatccctacc cggatggccc
       61 accgccgcag ccgccggccg ggttttttcc gtttttgtgg gcggccagtg cgggggtgcg
      121 cggcttgatt gtggcgatga cgctgctgac cgccaccatc ggggtgttcg aggccttgct
      181 gttttccatg ctgggctcgg tggtggattg gttaagccag acgccggcct cgcagttgtg
      241 ggcgcagcaa aaggggcatt tattgttgct ggcggggatt ttgctggcca gccctttgct
      301 ggtgacggtg cagtcgctgt gcaaattcca gggtttgcag gggaatttcc ccatgcgcct
      361 gcgctggaat ttccaccgcc atctgctcgg ccagagcatg cgcttttatc aggacgagtt
      421 tgccggacgg gtgtcggcca aggtgatgca aaccgcgctg gcggtgcgcg acaccatgat
      481 gattgtcggt gacatcatgg tgttcatcac catttacttt gtcaccatgg tggcggtggt
      541 cggcgggttt gacctggcct tgatgacgcc gtttctgggc tggctggccg gctatgtgct
      601 ggcgctgaaa atctttatcc cccggctcag ccgcgccgcc agcgcgcagg cggatgcgcg
      661 cagcctgatg accgggcgga ttaccgatgc ttacaccaat atcgccacgg tgaagctgtt
      721 ttcccaaggc gagcgcgagg cggggtttgc tcgggcggcg atgcaggatt tcatgcggac
      781 ggtgtaccgg cagatgcgcc tggccagcag ttttgagatc gtcaaccaca cgctgagcat
      841 gctgctgatt gcctgcaccg ctggcgcggc cttgtggcgc tggagcgagg gcgaagtaag
      901 cattggcgcg gtggcggcgg ccaccgccat ggcgctgcgg ctgaacggga tttcgcattg
      961 gatcatgtgg gagatgtcca atctgttcga gcatatcggc acggtgcagg acggcatcag
     1021 cacgctgtcc accccggtgc gcgtcaccga ccgcgccgac gccagcgcct tgcaagtgac
     1081 gcgcggcgaa atccgctttg agcaggtgag ctttagctat ggcggcgaca aaaacgtcat
     1141 cgaccaactc gacctcacca tccgccccgg cgaaaaaatc ggcctggtgg ggcgcagcgg
     1201 cgcgggcaaa tccacgctgg tgaatttgct gctgcgcttt gacgacgtcg gcgccgggcg
     1261 gattttgatc gacggccagg acatcgcccg cgtcagccag gacagcctgc gcagccaggt
     1321 gggcatggtc acccaggaca cctcgctgct gcaccgctcg gtgcgcgaca atttatgcta
     1381 cggccgcccc gaggccagcg acgccgagat ggtcgccgcc gcccgccgcg ccgaggcgca
     1441 tgagtttatc ttgggcctgg gcgacccggc gggccggcgc ggctacgaag cgcacgtcgg
     1501 cgaacgcggg gtaaagctgt ccggcggcca gcgccagcgc atcgccattg cccgagtgat
     1561 gctgaaaaac gcccccatcc tgctgctgga cgaagccacc agcgcgctgg attccgaggt
     1621 ggaagccgcc attcagggca gcctgtatca actgatggaa ggcaaaaccg tggtggcgat
     1681 tgcccaccgg ctgtccacca ttgccgccat ggaccggctg atcgtgctgg accagggccg
     1741 tattgtcgag cagggcgacc acgccagcct gctggcgcag ggtgggctgt acgcccgctt
     1801 gtgggcgcac cagagcggcg ggtttttggc ggaaggggcg gagtaaacgt cgcgccgggc
     1861 aaggcgcagg cccatcaaaa aaccgcgaca gagatcgtcg cggttttttg atgggcggga
     1921 aacactcagg cttcttcttt gcccggtttc ggttccggcg gaatcaccac ttcgctgtag
     1981 ccgcattctt ttt
//
LOCUS       DAOPYX010000412         1986 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_85115_length_1986_cov_10.838426, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000412 DAOPYX010000000
VERSION     DAOPYX010000412.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 1986)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1986)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1986
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85115_length_1986_cov_10.838426"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..857)
                     /locus_tag="PKN55_13985"
     CDS             complement(<1..857)
                     /locus_tag="PKN55_13985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011137397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HNX72403.1"
                     /translation="MILSMTGFASTSREFPGGLLAMELRAVNHRYLDVQLRLPEELRV
                     IEPQLREQIAAAVSRGKLECRVTLAQSGSDAPRMELNPLVLDSLLDVVAQLKARLPGA
                     KDLGTGELLRWPGVLNSNSLQPEALQQLCLGQLSALLADFNATRAREGEKLADILRSR
                     ITAIETIVAHVKPKLPAILSAWQAKLSSRLQEALGSVDDDRLKQEFALFAQKIDVDEE
                     LSRLVAHLSEVKRILKQGGQAGKRLDFLMQELNREANTLGSKSVSTETTQASVELKVL
                     IEQMREQIQ"
     gene            844..1116
                     /locus_tag="PKN55_13990"
     CDS             844..1116
                     /locus_tag="PKN55_13990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX72404.1"
                     /translation="MLKIIKKTPQKAAHTPSARPRASSGAMSMNSKGIAASAGVFAAI
                     QPVTSPPTRDGINKRPGNETINSGAGFHYNIHAVPGVSIQIKKNAI"
     gene            1189..1905
                     /gene="rph"
                     /locus_tag="PKN55_13995"
     CDS             1189..1905
                     /gene="rph"
                     /locus_tag="PKN55_13995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008953494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HNX72405.1"
                     /translation="MRPSLRSADALRPVRFTRHFTRHAEGSVLVCFGDTQVICTASVE
                     ENVPGFLKGKGQGWVTAEYGMLPRSTGSRMKREAASGKQSGRTQEIQRLIGRSLRAVV
                     DLKKLGERQIVIDCDVIQADGGTRTASICGAWLALHDAIAGLLARGALSESPLLDHVA
                     AVSVGVYQGQPVLDLDYPEDSACDTDMNVVMTGAGGFVEIQGTAEGAPFSREEMAQLL
                     ALAEQGITELVALQKAALAD"
BASE COUNT          384 a          620 c          604 g          378 t
ORIGIN      
        1 ttctgaatct gctcgcgcat ttgctcaatc agcactttca gctcaaccga agcctgcgtg
       61 gtttccgtag aaacagactt cgagcccagg gtgttggctt cgcggttgag ctcttgcatc
      121 aggaaatcca gccgcttgcc ggcctgaccg ccctgcttga ggatgcgttt gacttcagac
      181 agatgcgcca ccagacggga tagctcttcg tccacgtcga ttttttgcgc aaacagggcg
      241 aactcctgct tcaggcggtc gtcgtccaca ctgcccagcg cctcttgcag gcgactgctg
      301 agcttggctt gccaggccga cagaatggcc ggcagcttgg gcttgacgtg ggcaacgatg
      361 gtttcgatgg cggtgatgcg cgagcgcaga atatccgcca gtttttcgcc ttcgcgcgcg
      421 cgggtggcgt tgaaatcggc cagcaaggcg gagagctggc ccaggcacag ctgttgcagc
      481 gcttcgggtt gcaggctgtt gctgttgagc acgcccggcc agcgcagcag ctcgcccgtg
      541 cccaggtctt tggcgccagg caggcgcgcc ttcagttggg cgacgacgtc cagcaagctg
      601 tccagcacca gcgggttaag ctccatgcgc ggggcatcgc tgccggactg agccagtgtc
      661 acccggcact ccagcttgcc gcggctgacc gcagcggcga tttgctcacg caattgcggc
      721 tcgattaccc gcagttcttc cggcaggcgc agttgtacgt ccagatagcg gtggttgacc
      781 gcacgcagtt ccatcgccag caaaccaccg ggaaactccc ggctggtcga ggcaaaaccg
      841 gtcatgctca aaatcatcaa gaaaactcca caaaaagcgg cccatacccc ttctgcccgc
      901 ccgcgcgcca gcagtggcgc catgagcatg aacagcaagg gaattgcggc atcggcaggg
      961 gtattcgccg ccattcagcc ggtcacaagc ccgccaacca gggacgggat caacaagcgg
     1021 cccggaaatg agacgatcaa ttcaggcgcc ggatttcact ataacatcca cgctgttccg
     1081 ggtgttagca tccagatcaa gaaaaatgcc atatagctaa cttgacatgg ctaccgcgcg
     1141 agctgtcaag gttttgtata atctgcattt ttcttgctca ggatcgccat gcgcccctct
     1201 cttcgttctg ccgacgccct gcgtccggtg cgttttaccc gccacttcac ccgccatgcc
     1261 gaaggctcgg tgctggtatg ttttggcgac acgcaagtga tttgcaccgc cagtgtggaa
     1321 gaaaacgtcc ccggttttct caagggcaag ggccagggct gggtgacggc cgaatacggc
     1381 atgctgccgc gctccaccgg cagccgcatg aagcgcgaag ccgccagcgg caagcaaagc
     1441 ggccgcaccc aggaaatcca gcggctgatc ggccgcagcc tgcgcgcggt ggtggacctg
     1501 aaaaaactgg gcgaacgcca gattgtcatt gattgcgatg tgatccaggc cgacggcggc
     1561 acccgcaccg ccagcatctg cggcgcctgg ctggccttgc acgacgccat cgccggcttg
     1621 ctggcgcgcg gcgcgctgag cgaatcgcca ctgctggacc atgtcgccgc cgtgtcggtg
     1681 ggcgtgtatc agggccagcc ggtgctggat ctggattacc cggaagattc cgcctgcgac
     1741 accgacatga atgtggtgat gaccggcgct ggcggctttg tcgaaatcca gggcacggca
     1801 gaaggcgcac cgttcagccg cgaagaaatg gcccaattgc tggcgctggc ggaacaaggc
     1861 attaccgaat tggtggcgct gcaaaaggca gcgctggcag actgacaccg cccataagag
     1921 cggctcacac aaccctcctg gcgtcgttgc ccgaccttgc cgtacttgtt gtactgtcta
     1981 cggtcg
//
LOCUS       DAOPYX010000413         1985 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_85170_length_1985_cov_35.015544, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000413 DAOPYX010000000
VERSION     DAOPYX010000413.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 1985)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1985)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1985
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85170_length_1985_cov_35.015544"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(420..1646)
                     /locus_tag="PKN55_14000"
     CDS             complement(420..1646)
                     /locus_tag="PKN55_14000"
                     /inference="COORDINATES: protein motif:HMM:NF033592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HNX72406.1"
                     /translation="MDFIARHRQAARDFTRRAVFTFERVVGVLLVNLMRSLQVELDQF
                     FSRISLPLGRRAHDDAFRMARKKLRWQAFVELNQAVLADLHPAPDWHGLRVVACDGTT
                     LYLPTTHPDTISSGPDGFNCYHSQDGIYSLARASALCETSSGLILHASLAADTRDERS
                     MLVEQFEHLRPNDLLVLDRGYPAYWLFALLLARQQHFCIRLPQSFSPQVQAFVASGQA
                     CAVIKMQPGHGQRQDFVQHQLPMDAFSVRLVRVPLKTGQIEILATSLLDASRWPAADF
                     AALYQQRWRIEEAFRHLKCRLQLEQFGGETPQAIRQEFHASILLHNLAIIAAQDVLAE
                     QELDAATLAPNLTHATHLVRLYLPQLLEDPTSIDRIGPALFAGIAGQISKRRPGKPAP
                     PRKPNRKKPRRHRAYK"
BASE COUNT          434 a          516 c          583 g          452 t
ORIGIN      
        1 tgctgatgct gatgctgctg ctgttgctgc tgctgttgct gttgctgttg ttgctgttgc
       61 tgctgttgct gctgctgttg ctgctgttgc tgctgttgct gctgcttttg caagtgcaga
      121 tgcaggtgcg gctgcaggtg tgggtacagg tgcagctgca agtatgtttg tgcttataaa
      181 atccagaacc agtataggtg tgcatttttt tctgccatta gtcctgccag ccctcacact
      241 caaccttttc atccgcgctg tattcatccg tctttgggcg acccttcgat attggcgctt
      301 aggcttagcc gcttttccac ataattggcg cgaaacagtt tgtgttattg atgcccggca
      361 tctgccggag ctcttgccgg gcactgccgc gaaaatagcc accccgcata accctgcgat
      421 catttataag cccggtgacg gcgtggtttc ttccggtttg gcttacgtgg cggtgcgggc
      481 ttgccaggcc gtcgtttgct gatctgccca gcaattccag caaacaaggc tggcccaatc
      541 ctgtcgattg atgtcgggtc ttccagcaac tgcggcagat acaaacgcac cagatgtgta
      601 gcgtgagtca ggtttggcgc cagcgtggcc gcatccagct cttgttctgc caacacatcc
      661 tgcgcagcaa tgatggccag attgtgcaac aagattgagg cgtggaactc ctgacgaatc
      721 gcctgtggcg tctccccgcc aaactgctcc agttgcaagc gacatttgag atgccggaac
      781 gcctcttcga tgcgccagcg ttgctgatac agtgctgcaa agtctgcagc gggccagcgt
      841 gacgcatcca gcagcgacgt ggccagaatt tcaatctggc ctgttttgag cggaacacgc
      901 accaagcgca ctgagaatgc atccatcggc agctgatgct ggacaaaatc ctggcgctgc
      961 ccgtggccag gctgcatctt gatgacagca caagcctgac cggacgccac aaaagcctga
     1021 acctgtggcg aaaaactctg tggcagacga atgcagaaat gttgctggcg cgccagcaac
     1081 aaggcaaaca gccaataagc cggatacccg cgatccaaca ccaacaaatc gttggggcgc
     1141 agatgctcaa actgttcgac caacatgctg cgttcatcac gggtgtcggc agccagactg
     1201 gcgtgcagaa tcaggcctga gctggtttca cacaaggcgc tggcgcgagc cagactgtag
     1261 atgccgtcct gcgagtggta gcagttgaat ccatctgggc cgctgctgat ggtgtcaggg
     1321 tgtgtggtgg gcagataaag tgtggtgcca tcgcaggcaa ccacacgcaa gccatgccaa
     1381 tctggcgcgg gatggaggtc tgccagaacc gcctggttaa gttcaacaaa ggcttgccaa
     1441 cgcaacttct tgcgcgccat gcggaaagcg tcgtcatgag cgcgacggcc caagggcaag
     1501 gaaatccggg agaagaactg atcaagctcg acttgcagcg agcgcatcag attgacgagc
     1561 aagacgccga cgacgcgctc gaaggtgaaa acggcgcggc gggtaaagtc gcgagcagcc
     1621 tggcgatgtc gggcaatgaa gtccacggac gtgatgaggg tgtgaatttt atagattgaa
     1681 ctggagtact gagaggggcg gtttttttgt catttttaca tactgatcaa ttggttgtgc
     1741 gcgccattct accgtaaggg aaggttgcgc ggtgtatgat ttttagctaa tttcgcggta
     1801 gtggacaggg aaggccggcc gtgcgacgac gccaggagga tttggtgagc ggtgcttaac
     1861 cgcatgccat gaaaaaacag tgcttggcga tgctaaaatt gcctgacagt gccgaacgag
     1921 atcataaaaa tgtcgtggtc taatcttttc gaacagcccg ataggtggaa caatccccca
     1981 cccca
//
LOCUS       DAOPYX010000414         1948 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_87700_length_1948_cov_5.732699, whole genome shotgun sequence.
ACCESSION   DAOPYX010000414 DAOPYX010000000
VERSION     DAOPYX010000414.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 1948)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1948)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1948
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87700_length_1948_cov_5.732699"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..170
                     /locus_tag="PKN55_14005"
     CDS             <1..170
                     /locus_tag="PKN55_14005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012698654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HNX72407.1"
                     /translation="YTWWSNRGQAYAKDVGWRIDYQIATPALAARAHSASVYKGEKFS
                     DHAPLIVDYTE"
     gene            401..1708
                     /locus_tag="PKN55_14010"
     CDS             401..1708
                     /locus_tag="PKN55_14010"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing methyl-accepting
                     chemotaxis protein"
                     /protein_id="HNX72408.1"
                     /translation="MFIRKAQSERALTQQRHAFEQQLAVCRDELNAIRQHLACIEFSP
                     DGVILDANARFLALVGYRLEDIRGQHHRMFCEPSYAAGPEYAAFWRQLAQGQSQHATF
                     RRLAKSGRVIWLEASYFPVRQADGQVVKVVKLASDVTDSHFALLEKTAMFTALNHSLA
                     VIEFKPDGTILTANDNFLRTTGYSLAQIQGKHHAMFCTDNFYRENPNFWKQLAAGHFM
                     SGRFERRNSAGERVWVEATYNPIVDADGKVYKVIKFASDITNRVMAKLEAATVATDAS
                     RQTSQIAHEAREKLDEVMGISDEIARYASEATAVSARLDAQIHSINDIVATIQSIANQ
                     TNLLSLNAAIEAARAGESGRGFAIVADEVRKLAARTSEATTEIGSVAHANTELTQQIA
                     SQIDRINTISTSGQAKVQHVCTRIAGVDDSVANLLDVVARLEE"
BASE COUNT          405 a          578 c          568 g          397 t
ORIGIN      
        1 gctacacctg gtggagcaat cgcggccagg cctacgccaa ggacgtgggc tggcgcattg
       61 attaccagat tgccaccccg gcgctggcgg cacgggcgca cagcgccagc gtgtacaagg
      121 gtgagaagtt ttctgaccat gcgccgttga ttgtggatta caccgagtaa tgctggccgg
      181 gcctccctcg atgtgtggcg gcccgctgtc gatgacctgt gcccgcgccc aatcacgttt
      241 catggtgaaa aacatgtgtt taatagcaag gggagaatgc gggcaactat atgaattttc
      301 ggttaattac tgagcgtata aatgcgctgg acagcactgg aagccttatc catactcaag
      361 atggattctg tatctatgcc agacctacca aggaggctcc atgttcatcc gcaaagccca
      421 atccgagcgc gccctcacac agcaacgcca cgcttttgag cagcaactgg ccgtgtgtcg
      481 cgatgaactg aatgccattc gccaacatct ggcgtgcatt gaattttccc ctgacggcgt
      541 gattctggat gccaacgcac gctttctggc cttggtgggc tatcggctgg aggatatacg
      601 tggtcagcat caccgcatgt tctgcgagcc gagttatgcc gccggcccgg aatacgcagc
      661 cttctggcgt cagctggcac agggccagtc ccagcacgct acttttcgtc gcctggccaa
      721 gtccgggcgc gtcatctggc tggaagcctc atactttccg gtgcgccaag ccgatggcca
      781 ggtagtgaaa gtggtcaagc ttgcctcgga tgtcaccgac agccactttg ccctgctgga
      841 aaaaaccgcc atgttcaccg cgctgaacca ttcactggcg gtgattgagt tcaagcctga
      901 cggcaccatc ctcacggcca acgacaattt tttgcgcacc accggctata gcctggcgca
      961 aattcagggc aagcatcatg cgatgttctg caccgacaat ttttaccggg aaaacccgaa
     1021 tttctggaag cagctggccg ccggacattt catgtccggt cgtttcgagc ggcgcaacag
     1081 tgctggcgag cgcgtctggg tggaggcgac gtataacccg atcgtcgatg ccgatggcaa
     1141 ggtgtacaag gtgatcaaat ttgcttcaga catcaccaac cgggtgatgg ccaagctgga
     1201 agccgccacg gtggctactg atgcctcccg gcaaacctcg cagattgccc acgaggcgcg
     1261 ggaaaagctg gacgaagtga tgggcatctc ggatgagatt gcccgttacg ccagcgaagc
     1321 cactgccgtc agcgcccggc tggacgcgca aatccacagc atcaatgaca ttgtcgccac
     1381 catccagagt atcgccaacc agaccaattt gctgtcgctg aatgctgcca ttgaggctgc
     1441 gcgtgcgggt gagagcggcc gtggctttgc catcgtggcc gatgaggtgc gcaagctggc
     1501 cgccagaaca tcagaagcga cgaccgaaat cggcagcgtg gcgcacgcca acaccgagct
     1561 gacccagcaa atcgcctcgc agatcgaccg catcaatacc atctccacca gcggtcaggc
     1621 caaggtgcag catgtctgca cacgtattgc cggggtggac gacagcgtgg ccaatttatt
     1681 ggatgtggtg gcgcggctgg aagaataacc ctgccagctg ggcagcgggg gcagccacat
     1741 cgccgcccgg tgcccggatc aggcgcgcgc gctctttaca cccgaaagct cgacaatgtc
     1801 tggttaagca tgcccgacac ctgtcccagt tcatcggcct tgttgcgcat ctgctcggcc
     1861 tgacggttgt tgtcctgcat ggcgttgttg atctgctcca gttcgccgcc cacggcttcg
     1921 atgtcatggc ggatgctttc ggcgctgg
//
LOCUS       DAOPYX010000415         1931 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_88906_length_1931_cov_12.735075, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000415 DAOPYX010000000
VERSION     DAOPYX010000415.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 1931)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1931)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1931
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88906_length_1931_cov_12.735075"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            137..212
                     /locus_tag="PKN55_14015"
     tRNA            137..212
                     /locus_tag="PKN55_14015"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:170..172,aa:Ala,seq:ggc)
     gene            324..635
                     /locus_tag="PKN55_14020"
     CDS             324..635
                     /locus_tag="PKN55_14020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX72409.1"
                     /translation="MHPPELSDYTALFARYGKLSDIYMGPDDERFRLLFEQVCHLLSQ
                     PSPFNLSLPDGFVRTARQYLANDSRTVSHMRNPENRHFMLSDLFDYVELMQRIHARRH
                     P"
     gene            673..>1931
                     /gene="radA"
                     /locus_tag="PKN55_14025"
     CDS             673..>1931
                     /gene="radA"
                     /locus_tag="PKN55_14025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HNX72410.1"
                     /translation="MAKAKTVYVCSECGGISPKWQGQCPHCTSWNSLSETVQAPPAAN
                     GGNHRFQALAASSGEVQVLAEVQAEEAPRDPSGSDELDRVLGGGLVRGAVILIGGDPG
                     IGKSTLLLQTLASVGARRKVLYVTGEESAQQVALRASRLAVDAGRVRLLAEIRLEQVL
                     AVLQSERPDVVVIDSIQTVYTDVLSSAPGSVGQVRECAAQLTRVAKQTGVTMILVGHV
                     TKEGALAGPRVLEHIVDTVLYFEGDSHSSFRMIRAIKNRFGAVNELGVFAMTDRGLKG
                     ISNPSAIFLSSHRDEVPGSSVLVTMEGTRPLLVEIQALVDEAHGQPRRLSVGLEQNRL
                     AMLLAVLHRHAGVACFDQDVFINAVGGVRIGEPAADLAVLLAIVSSLRNRPLPDKLVV
                     FGEVGLAGEVRPVAHGQERLKEAAKLG"
BASE COUNT          344 a          580 c          626 g          381 t
ORIGIN      
        1 gagcgcttgc atggcatgca agaggtcagc ggttcgatcc cgcttacctc caccaccaaa
       61 ttcgattgac tgcgttaagc agttgatcag gcagttaaaa atcctgagtc tgaccagatc
      121 aggtgcaccc gacagtgggg gtatagctca gctgggagag cgcttgcatg gcatgcaaga
      181 ggtcagcggt tcgatcccgc ttacctccac cacttcatca aaaatcacgc caccttcggg
      241 tggcgtgatt ttttgcgctg caaaatcatg acaaaactgt tataagaagc gccacccctt
      301 cttaactcta ttgcgagtct gtcatgcatc cccccgagct ttccgactac accgccttgt
      361 ttgcccgtta tggcaagcta tctgatattt atatgggccc ggacgacgag cgctttcgct
      421 tgctgttcga gcaggtctgt catctgctca gccagccttc gccgttcaat ttgagcctgc
      481 cggatggctt tgtgcgcacc gcccgccagt atctggccaa cgacagccgc acggtcagcc
      541 atatgcgcaa cccggaaaac cgccacttca tgctcagcga tctgtttgat tacgtggagc
      601 tgatgcagcg cattcacgcg cgccgccacc cgtaagggtg ttttactgat ctgattgatt
      661 gagcgtcacc ccatggccaa agccaaaact gtttatgtgt gcagcgaatg cggcggcatc
      721 agccccaaat ggcagggcca gtgcccgcac tgcaccagct ggaactccct gagcgaaacc
      781 gtgcaggcgc cgcctgccgc caacggcggc aatcaccgct ttcaggcgct ggccgccagc
      841 agcggcgaag tgcaggtgct ggccgaggtg caggccgaag aggcgccgcg cgacccgtcc
      901 ggcagcgacg agctggaccg ggtgctgggc ggcggcctgg tgcgcggcgc ggtgatcctg
      961 attggcggtg accccggtat tggcaaatcc accctgctgc tgcaaaccct ggccagtgtc
     1021 ggcgcgcggc gcaaggtgct gtatgtgacc ggcgaagaat ccgcccagca ggtggcgctg
     1081 cgcgcctcgc ggctggcggt ggacgccggg cgggtgcgcc tgctggcgga aatccgcctg
     1141 gagcaggtgc tggcggtctt gcagagcgag cgcccggacg tggtggtgat tgactcgatt
     1201 caaaccgtgt acaccgatgt gctcagctcc gcccccggct cggtgggcca ggtgcgcgaa
     1261 tgcgcggcgc agctgacccg agtggcgaag cagaccgggg tgaccatgat tctggttggc
     1321 cacgtcacca aagaaggcgc gctggccggc ccgcgcgtgc tggaacacat tgttgacacc
     1381 gtgttgtatt ttgagggcga cagccattcc agttttcgga tgatccgcgc catcaaaaac
     1441 cgctttggcg cggtcaacga gctgggcgtg ttcgccatga ccgaccgtgg gctgaagggg
     1501 atttccaacc cgtcggcgat ttttttgtcc agccaccgcg acgaagtgcc tggctccagc
     1561 gtgctggtga ccatggaagg cacccgcccc ttgctggtgg aaatccaggc gctggtggac
     1621 gaagcgcacg gccagccgcg ccgactgtcg gtggggctgg agcaaaaccg cctggccatg
     1681 ctgctggcgg tgctgcaccg ccacgccggg gtggcctgtt ttgatcagga tgtgtttatc
     1741 aatgcggtgg gcggcgtgcg cattggcgag ccggcggctg acctggcggt gttgctggcc
     1801 attgtctcgt cactgcgcaa ccggcccttg ccggataaat tggtggtgtt tggcgaggtg
     1861 ggcctggccg gtgaggtgcg cccggtggcg cacggccagg agcggctgaa agaagccgcc
     1921 aagctgggct t
//
LOCUS       DAOPYX010000416         1912 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_90329_length_1912_cov_4.352181, whole genome shotgun sequence.
ACCESSION   DAOPYX010000416 DAOPYX010000000
VERSION     DAOPYX010000416.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 1912)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1912)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1912
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90329_length_1912_cov_4.352181"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            57..686
                     /locus_tag="PKN55_14030"
     CDS             57..686
                     /locus_tag="PKN55_14030"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNX72411.1"
                     /translation="MTHRDTTDSVRARLLQAALDCFLADDYHQVSTRRIAEQANANVA
                     MIRYYFGNKEGLYEEMIRDTLAPLLSVLDGPMLNSVTGFTEFLQLYYQTMAEHPAFPK
                     LILKVLALNQGPGRRFIQQLLERGRTQGAHKLAALKASGHLSADLDPDIVRMSFVSLA
                     MTPMLLKTIFENQMERTMDADFLQQLAAFNGHLLAAGLSGAGKTMTGAA"
     gene            734..1804
                     /locus_tag="PKN55_14035"
     CDS             734..1804
                     /locus_tag="PKN55_14035"
                     /inference="COORDINATES: protein motif:HMM:NF013087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HNX72412.1"
                     /translation="MKFAAWLHAIPHALTPAPFRLMQIGSAFWQSRALYVAAQLDIAT
                     QLGDSAQHSSDLAAQLQLDADALGRLLRLLAAIGVFQETKPMVFANNNVSACLRSDHP
                     KSVRAMILLHNADAMSRPWFEHLEHGVRTGQSPFVCGHGETLFSYLDHHPQFDQLFST
                     AMDCVEALAGDSFATDFDWRQFDRLLDIGGSRGSKAHTILTRHPHLRALVVDRPPVIA
                     EALEYWAAHPDAICARMAFEACDILDTMPLAHNSRDIYFLSAILHGFDDAHCLRILHK
                     LAKACRHSGARVVLLEMVLPEKGADQTQAAFDMQMFMGGSGRERTLTEWQALFAQSGM
                     TLSDDVRLRSFAHMLVLRPTQQ"
BASE COUNT          408 a          560 c          526 g          418 t
ORIGIN      
        1 ggtcagggcg cacacaagtg ctaacctctt caacatcatt ctgagagagc ttagccatga
       61 cccaccgtga tacaacagat tccgtgcggg ctcgcctgct gcaggccgca ctggattgct
      121 ttctggcaga tgattaccat caggtcagca cccggcgcat tgccgagcag gccaatgcca
      181 atgtcgccat gattcgctat tacttcggca ataaagaagg cctgtatgaa gaaatgattc
      241 gggacacctt ggcgccactg ctgtcagtgc tggatggccc catgctgaac tcggtcaccg
      301 ggtttaccga atttctacag ctgtattacc aaaccatggc cgaacacccg gcatttccca
      361 agctgatcct caaagttttg gccttgaatc aaggcccagg ccgacgcttt atccaacaac
      421 tgctggagcg aggacggacg caaggcgcgc ataaattggc ggccctgaaa gccagcggac
      481 atttgtctgc agacctcgat ccggacattg tgcgcatgag ctttgtcagc ctggccatga
      541 cgccgatgct gctgaaaacc atttttgaaa accaaatgga gcggaccatg gatgctgact
      601 ttttgcagca attggccgca ttcaatggcc atctgctggc ggcaggtttg tccggcgcgg
      661 gcaaaaccat gacgggagcg gcgtgatgag cctacaaaaa aacactgggg cagtgcgccg
      721 ttttgcctgg ctgatgaaat ttgccgcctg gttgcacgcc attccacacg cgctgacccc
      781 tgccccattt cgtttgatgc aaatcggttc ggcattttgg caatcccgcg ctttgtatgt
      841 ggccgctcaa ttggatatcg ccacacagct tggcgattcg gcgcaacata gcagtgattt
      901 ggctgcgcaa ttgcagctgg atgccgatgc gctcgggcga ctgctgcgcc tgctggccgc
      961 catcggcgtg tttcaggaaa ccaaaccgat ggtttttgcc aataacaacg tatctgcgtg
     1021 cctgcgaagc gaccacccca agagtgtgcg ggccatgatt ctgctgcata acgccgacgc
     1081 catgagccgc ccttggtttg agcacctgga acacggcgtg cgcaccgggc agtcgccttt
     1141 tgtgtgcggc cacggagaga cgctattttc atatctggat catcaccccc aatttgacca
     1201 actattctcc accgccatgg actgcgtcga agccctggca ggcgacagct ttgccaccga
     1261 cttcgattgg cggcagtttg accgcctact cgatattggt ggttcacgtg gcagcaaagc
     1321 gcacacgatt cttacccgcc atccacactt acgcgccctg gtggtggatc gtccgcccgt
     1381 gattgccgaa gcgctagaat attgggcggc gcacccggac gccatctgcg ctcgaatggc
     1441 gtttgaagcc tgcgacatcc tggacaccat gcctttagcg cataacagcc gggatattta
     1501 ttttctgtca gcaattttgc atgggtttga cgacgcacac tgcctacgca ttctgcacaa
     1561 gctcgccaaa gcctgccgcc acagcggcgc acgggtcgtg ctgctggaga tggtgttgcc
     1621 ggaaaagggc gcagaccaaa cacaagcagc gtttgacatg caaatgttca tgggcggcag
     1681 tgggcgtgag cgcaccctga ccgaatggca ggctttgttt gcccaaagcg gcatgacatt
     1741 gagcgacgat gtgcgcctgc gcagctttgc ccatatgttg gtgctgcgcc ctacgcaaca
     1801 gtgaagccgt gaaaacacac agcgggcagc agattatttg cgccgacctg gcccctgccc
     1861 gtataatcga cggttttcgc aacccttgcc ttcccgctac gcatggcgga ag
//
LOCUS       DAOPYX010000417         1910 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_90470_length_1910_cov_9.915364, whole genome shotgun sequence.
ACCESSION   DAOPYX010000417 DAOPYX010000000
VERSION     DAOPYX010000417.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 1910)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1910)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1910
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90470_length_1910_cov_9.915364"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..296
                     /locus_tag="PKN55_14040"
     CDS             <1..296
                     /locus_tag="PKN55_14040"
                     /inference="COORDINATES: protein motif:HMM:NF025619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4351 domain-containing protein"
                     /protein_id="HNX72413.1"
                     /translation="HNIEVLEEQEKMRYVTSVEQIGIEKGLLQGMQQGMQRGLMQGRA
                     EGEAYALRRLLQKRFGPLSEDVLARLQSASIDELELWLDRALDADSLAGVFAQ"
     gene            502..1788
                     /locus_tag="PKN55_14045"
     CDS             502..1788
                     /locus_tag="PKN55_14045"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HNX72414.1"
                     /translation="MHTLSLSHTPDLLALAAAEPAHFPCLLQSAGSQGWDILAAFPED
                     VRVYGAGQAAELAADLAALPVGDGWATGELPFRGGWFFYLGYELLEQFEPSVPVGLED
                     DFPLGALLRVPAAILVNRADASAWLVAEHREQLDRLIASLAEAPAFNPRAPQVARVQE
                     DPPQAFTDAVRRIQRYIREGDVFQVNVSRQWDVTLAQALSPAEVFAALRLANPAPFSA
                     LLQLGEHAVVSSSPERLVRVVDGWADTRPIAGTHPRSPDPVEDAALRQRLIDNAKERA
                     EHIMLIDLERNDLGRICLPGTVEVNELMAVASYSYVHHIESNVRGRKRHEVSPAQVMR
                     ALFPGGTITGCPKVRTMQIIRELETAPRRAYTGSLGYLNHDGTLDSNILIRTFMHTGA
                     QLRFRAGAGIVADSDPERELQETRHKARGLLRALGQ"
BASE COUNT          314 a          576 c          636 g          384 t
ORIGIN      
        1 ggcacaatat cgaagtgctt gaggagcaag aaaaaatgcg ttacgtgact tctgtagagc
       61 agatcgggat tgaaaaaggc cttttgcagg ggatgcaaca aggcatgcag cgggggttga
      121 tgcagggccg agccgaaggc gaggcctacg cactgcgccg tctgctgcaa aaacgctttg
      181 gcccgttgtc cgaagatgtg ctcgcccgtt tgcagagcgc cagcatcgac gagctggaat
      241 tatggctgga tcgcgccttg gatgcggaca gtctggccgg ggtgtttgct cagtaagcat
      301 tgctatcaag ccctcctggc gttgtggtac ggcgttgtcg cacgacttgt gttttctgcg
      361 cgcatctttg cactgcgtat tgtcagctcc cgttgccgcc ctggggtttt ggcgtgtcag
      421 cttgcatgct gttgaccgcg cttgacagcc ggggcggccg tgttaccctc agcccggttt
      481 tcttattctt ttgttggttc aatgcacacc ctttcgctct cccatacccc tgatctgctt
      541 gccctggctg ccgccgagcc tgcgcacttc ccttgcttgt tgcaatcggc gggcagccag
      601 ggttgggata ttctggcggc gtttcctgaa gacgtgcgcg tgtatggcgc cggccaggcg
      661 gcggagctgg cggcggatct ggccgccttg ccggtgggcg atggttgggc gacgggcgag
      721 ttgccgtttc gcggcggctg gtttttttat ctgggttatg agttgctcga gcaatttgaa
      781 cccagcgtgc cggtggggct ggaggatgat tttccgttgg gcgccttgtt gcgcgtgccg
      841 gcggcgattt tggtcaaccg cgccgacgcc agcgcctggc tggtggccga gcaccgcgaa
      901 caactggatc ggctgatcgc cagcctagcc gaggcgccgg ccttcaaccc gcgcgcgccg
      961 caggtggctc gcgtgcagga agacccgccg caggctttta ccgacgccgt gcgccgcatt
     1021 cagcgctata tccgcgaagg cgatgtgttt caggtgaatg tgtcgcgcca gtgggatgtg
     1081 acgctggcgc aggccttgag cccggccgag gtgtttgccg cgctgcggct ggccaatccg
     1141 gcgccgtttt cggcgctatt gcaattgggc gagcatgcgg tggtcagctc gtcgccggag
     1201 cggctggtgc gggtggtgga tggctgggcg gacacccggc cgattgccgg cacccatccg
     1261 cgttcgcccg accccgtcga agacgctgcc ctgcgacaac gcctgatcga caacgccaaa
     1321 gagcgcgccg agcacatcat gctgatcgac ctggagcgca atgacctggg gcgcatctgc
     1381 ctgccgggca cagtggaggt caacgagctg atggcggtgg ccagctacag ctatgtgcac
     1441 cacatcgaat ccaatgtgcg tgggcgcaag cgccatgagg tcagcccggc gcaggtgatg
     1501 cgcgcgctgt ttcccggcgg caccatcacc ggctgcccga aggtgcgcac catgcagatc
     1561 atccgcgagc tggaaaccgc gccacgccgc gcctacaccg gcagcctggg ttatctgaac
     1621 catgatggca cgctggacag caatatcctg atccgcacct ttatgcacac tggtgcgcag
     1681 ctgcgttttc gcgcgggcgc cggcattgtt gccgactccg atcccgaacg cgaattgcag
     1741 gaaacccgcc acaaggcgcg cggcctcttg cgcgcgctgg ggcagtaagc gccggcaaca
     1801 aacggccagg ccgcacgaca acgccaggaa gggtgcgtga gcggcgctaa gcccggtgca
     1861 tctggccgcc gtcagtcttg atccggcaaa aacagcccca gcaaatcatt
//
LOCUS       DAOPYX010000418         1900 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_91268_length_1900_cov_3.928997, whole genome shotgun sequence.
ACCESSION   DAOPYX010000418 DAOPYX010000000
VERSION     DAOPYX010000418.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 1900)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1900)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1900
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91268_length_1900_cov_3.928997"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..666
                     /locus_tag="PKN55_14050"
     CDS             <1..666
                     /locus_tag="PKN55_14050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018747625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HNX72415.1"
                     /translation="AGAGAQAGGSKAQINQLNAGRVPGGATVEREVPTVVGNGSSVSL
                     ELNEASFTTANRVVQAINTAFGPNTARAVDGRVVEVAAPQDPNQRVQFLAKMENIAVT
                     TADMSPTVILNARTGSIVMNQAVTVEPCAIAHGNLSVTVTNTPNVSQPNALSNGQTTV
                     TNQADIQINQDGGKLVSVPKGSNLSQIVKALNAVGATPQDLLAILQAMKAAGSLKADL
                     QVI"
     gene            736..1728
                     /gene="flgJ"
                     /locus_tag="PKN55_14055"
     CDS             736..1728
                     /gene="flgJ"
                     /locus_tag="PKN55_14055"
                     /inference="COORDINATES: protein motif:HMM:TIGR02541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HNX72416.1"
                     /translation="MQVNSRAATTPNDLSQSYVLDLNAVESLKTSAKQDPKNAIKQVA
                     GQFEAMFLNILMKSMRQTSFDGEEDSNALDTYQGMFDQQLVQQLAGSGRGTGLAAALE
                     QQLGKAAGVDMGADAGRATGGQVREMESLIQPMPQQAWSSANPRVRSALEGALAKLAE
                     PKVRMDNTAVAGNGGPVSATSPAEFSRSLLPHAQQAAQELGVAPQLVVAHAALESGWG
                     KKPIRHADGSDSHNLFSIKAGSSWKGRTVDILTTEYIDGAPQKRVERFRAYDNYQEAL
                     ADYAKLIKNNPRYQGALNQGGDAVAFAKGLARGGYATDPNYAAKLSQLAGKSAS"
     gene            1798..>1900
                     /locus_tag="PKN55_14060"
     CDS             1798..>1900
                     /locus_tag="PKN55_14060"
                     /inference="COORDINATES: protein motif:HMM:NF012674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body protein"
                     /protein_id="HNX72417.1"
                     /translation="MSGIFGIALSGLNAARTSLTTTSHNISGVNTAGY"
BASE COUNT          454 a          539 c          574 g          333 t
ORIGIN      
        1 gccggcgcag gcgcccaggc tggcggcagc aaggcacaga tcaaccagct caacgccggg
       61 cgtgtgccgg gcggcgccac ggtagaacgg gaagtgccca ccgtggtggg caatggctcg
      121 tcggtcagcc tggagctgaa cgaagccagc tttaccaccg ccaaccgggt ggtgcaggca
      181 atcaataccg catttggccc gaacaccgcc agggcggtgg atggtcgagt ggtcgaagtc
      241 gctgcgccgc aggaccctaa ccagcgcgtg caatttctgg ccaagatgga aaatattgcc
      301 gtcactacgg cagatatgtc ccccaccgtg atcctgaacg cccgcactgg ctccattgtg
      361 atgaaccagg ccgtgacggt tgagccatgc gcgattgccc atggcaatct gtcggttacc
      421 gtcaccaata cgccaaacgt cagccagccc aacgcgctgt ccaacggcca gactacggtc
      481 accaaccagg cggatatcca gatcaatcag gatggcggca agctggtcag tgtgcccaaa
      541 ggcagcaatc tgtcgcaaat cgtcaaggca ctgaatgccg tgggcgccac accgcaggat
      601 ttgctggcaa ttttgcaggc gatgaaagca gccggttcgc tcaaggcgga tctgcaagtc
      661 atctgatcac atcaaccgaa atatctggca cgttacttgc tgtgacgtac cggactctgc
      721 tcaggaaatg gcttcatgca agtaaactca cgtgcggcaa ccacgccgaa tgatctctcg
      781 caaagttatg tgctggatct taacgcggtt gaaagcctga aaacttcagc caagcaggat
      841 cccaaaaatg ccatcaagca ggtggccgga cagtttgagg cgatgttcct caatatcctg
      901 atgaagagca tgcgccagac ttcatttgac ggtgaagaag acagtaatgc gctggacacc
      961 tatcagggca tgttcgacca gcaactggtg cagcaactgg cagggagcgg ccgaggcaca
     1021 ggcctggcag ccgcgctgga acaacagttg ggcaaggccg ccggggtgga tatgggggcg
     1081 gatgctggcc gggccactgg cgggcaggtg cgcgaaatgg aaagcctgat ccagccaatg
     1141 ccacagcagg catggagtag cgccaacccc agagttcgtt ctgctctgga aggtgcgctg
     1201 gccaagctgg ctgagcccaa ggtcaggatg gacaatacgg cagtggcggg aaatggcggc
     1261 ccggtatcgg ccacctcacc ggcagaattc agccgctccc tgttgccaca cgcccagcag
     1321 gcagcacagg aacttggcgt ggcgccacaa ctggtggttg ctcatgccgc actggaaagt
     1381 ggctggggca aaaagcccat ccgtcacgcg gatggctcgg acagccataa cctgttcagc
     1441 atcaaggccg gctccagctg gaaaggcagg acggtcgaca ttctgaccac tgaatacatt
     1501 gatggcgctc cgcaaaaacg ggtagagcgc ttccgcgcct acgacaacta ccaggaagcc
     1561 ctggctgact acgccaagct gattaaaaac aacccgcgct accagggggc attgaatcag
     1621 gggggtgacg ctgttgcctt tgccaagggg ctggcgcgcg ggggctatgc gacagatcca
     1681 aactatgcag ccaagctgtc gcagctggcc ggcaaatctg ccagttgagt caggcaggaa
     1741 tgaatatcaa gttgatcgtc tgacggtcga taccagcagg atacaagggg atttattatg
     1801 tcaggtattt tcggaattgc tctcagcggt ttgaatgctg cacgcacctc actgacgacg
     1861 accagccata acatcagtgg cgtcaacaca gctgggtatc
//
LOCUS       DAOPYX010000419         1891 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_91916_length_1891_cov_11.155773, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000419 DAOPYX010000000
VERSION     DAOPYX010000419.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 1891)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1891)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1891
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91916_length_1891_cov_11.155773"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(79..726)
                     /gene="leuE"
                     /locus_tag="PKN55_14065"
     CDS             complement(79..726)
                     /gene="leuE"
                     /locus_tag="PKN55_14065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014088338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine efflux protein LeuE"
                     /protein_id="HNX72418.1"
                     /translation="MFGITDLTTYILGTIFIVLLPGPNSMYVLSVAAQRGVKAGFAGA
                     AGVFVGDFILMTLAATGAAGLLKANPALFAVVKYLGGGYLAWVGVNMLLGAWRAWRQR
                     QLAEASPARAVNAANPFVRALVISLMNPKAILFFVSFFVQFVDPNYAYPALTFAILGG
                     ILQCFSALYLTTVIIAGARLADAFRRRRKLAASLSGAAGAVFVGFGAKLASASLN"
     gene            complement(742..1875)
                     /gene="carA"
                     /locus_tag="PKN55_14070"
     CDS             complement(742..1875)
                     /gene="carA"
                     /locus_tag="PKN55_14070"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011137351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HNX72419.1"
                     /translation="MSTIPAILALADGTVFTGRAIGATGHTVGEVVFNTAITGYQEIL
                     TDPSYTRQLVTLTYPHIGNVGANAEDAESRAVFAAGLIIRDLPLLHSNFRAQESLGDY
                     LTRNGTVAIADIDTRKLTRLLREKGAQAGCIMAGEVDAARAVELARGFGSMAGQDLAK
                     VVSCAEPYAWTDREWALGHGYRAQTEPKFHVVAYDFGVKHNILRMLAERGCKVTVVPA
                     QTPAAEVLALNPDGVFLSNGPGDPEPCDYAIAAIRQVLDTRLPVFGICLGHQLLGLAT
                     GAKTSKMKFGHHGANHPVQDLDSGRVMITSQNHGFQVDETSLPANVRVTHRSLFDHTV
                     QGIALTDRPAFSFQGHPEASPGPNDVAYLFDRFTAAMAERQPA"
BASE COUNT          390 a          588 c          605 g          308 t
ORIGIN      
        1 caggatgctt tggatgtctg tacgtttggg cattcttgca ctctacacaa tcaatttatg
       61 aattcgaacg atttgctgtt agttcaggct ggcgctggcc agcttggcgc caaagccgac
      121 aaacaccgcg ccagcggcgc cagacagcga ggcggccagc ttgcggcggc ggcgaaacgc
      181 gtccgccagg cgggcgccgg cgatgatgac ggtggtcaga tacagcgcgc taaagcactg
      241 caaaatgccg ccaagaatgg caaaggtcag cgccggatag gcgtagttcg ggtcaacgaa
      301 ctgcacgaaa aaggacacaa aaaacaagat ggccttgggg ttcatcaagc tgatgaccaa
      361 agcccgcaca aaagggttgg cggcattcac cgcccgcgcc gggctagcct cggccaactg
      421 gcgctgacgc caggcgcgcc aggcgcccag cagcatattc acgcccaccc aggccagata
      481 gccgccgccc agatacttga ccacggcaaa cagcgccgga ttggctttca gcagcccggc
      541 ggcgcccgtg gcggccagcg tcatcaggat gaaatcaccg acaaacacgc cagcggcccc
      601 ggcaaaaccg gccttcaccc cgcgctgggc cgccacggac aacacataca tcgagttggg
      661 gcccggcagc agcacaataa aaatcgtgcc cagaatatag gtggtcaggt cggtgatgcc
      721 gaacatgctg tgctcccagg gtcaggccgg ctggcgttcc gccatggcgg ccgtaaagcg
      781 gtcaaacaga taggccacat cgttcgggcc cgggctggct tccgggtgac cctggaaaga
      841 aaacgccgga cggtcggtca gggcaatgcc ctgcacggtg tggtcaaaca ggctgcggtg
      901 cgtcacgcgc acattggccg gcaggctggt ttcgtccacc tggaaaccgt ggttctggct
      961 ggtgatcatc acccggccgc tatccagatc ctgcaccgga tggttggcgc cgtgatggcc
     1021 aaacttcatc ttgctggtct tggcgccagt ggccaggccc aagagctgat ggcccaggca
     1081 aatgccaaac accggcagac gggtgtcgag cacctgacga atggcggcaa tggcgtaatc
     1141 acacggctcg gggtcgcccg ggccgttgga caggaacacg ccgtccggat tcagcgccag
     1201 cacctcggcc gccggggttt gcgccggcac caccgtcacc ttgcagccgc gttcggccag
     1261 catgcgcagg atgttgtgct tgacgccaaa atcgtaagcc accacatgga acttcggctc
     1321 ggtctgcgcg cggtagccgt ggcccagcgc ccattcgcgg tcggtccagg cgtagggctc
     1381 ggcgcagctg accaccttgg ccagatcctg gccggccatc gagccaaagc cgcgcgccag
     1441 ctccaccgcg cgagcggcat ccacttcacc ggccatgatg cagccggcct gagcgccttt
     1501 ttcgcgcagc aggcgggtga gcttgcgggt gtcgatgtcg gcaatggcca ccgtgccatt
     1561 gcgggtgagg taatcgccca gcgactcttg tgcacggaaa ttgctatgca gcaagggcag
     1621 atcgcggatg atcaagccgg cggcaaacac ggcgcgcgac tcggcatcct cggcgttggc
     1681 gcccacattg ccgatatgcg ggtaagtcag ggtgaccagc tggcgggtgt aggacggatc
     1741 ggtaagaatt tcctgataac cggtgatggc ggtgttgaac accacttcac cgacggtatg
     1801 gccggtcgcg ccaattgcgc ggccggtaaa gacagtcccg tcagccaagg caagaattgc
     1861 gggaatcgtg gacactggct gctcctgata c
//
LOCUS       DAOPYX010000420         1888 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_92154_length_1888_cov_7.147845, whole genome shotgun sequence.
ACCESSION   DAOPYX010000420 DAOPYX010000000
VERSION     DAOPYX010000420.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 1888)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1888)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1888
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92154_length_1888_cov_7.147845"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            145..1143
                     /locus_tag="PKN55_14075"
     CDS             145..1143
                     /locus_tag="PKN55_14075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018746755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HNX72420.1"
                     /translation="MSPIAWPQRLRAILHKEWFLFGMLGAVALASLAPDIGRAGGPLH
                     GDVLADLGIAVVFFLHGLGISFASLRQGMLRWPVHLLVQLLTFAVFPLLWLLAAPLVS
                     AWMPAGLALGFCYLCALPSTISSSVAMTGLARGNVPAAIFNATLSSVLGIALTPLLVA
                     ALAHLGGGQGMDLGQAVLSIARLLLLPLLAGQLLRPLLHAWHQRYKRYTNLLDRWVIL
                     LLVYTAFCDSVASGLWRDHGLATLATAMLGAALALLVVLTLSTWLARRLGFSVEDEIT
                     TVFCGSKKTLASGVPMAKLLFGAHPAIGLIVLPIMFYHQIQLLVCAVLANRYAARR"
     gene            complement(1312..1731)
                     /locus_tag="PKN55_14080"
     CDS             complement(1312..1731)
                     /locus_tag="PKN55_14080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008953788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HNX72421.1"
                     /translation="MKAKLKWIDGVCFMGETGSGHAVVMDGAPEGGGRNLGPRPMELV
                     LLGTAGCTSYDVLSILRKSRQDVRDCWVELDAERAEVDPKVFTRIHFHFVVTGRGLKA
                     DAVERAIKLSAEKYCSASIMLAKTAEITHDFELREDA"
     gene            1691..>1888
                     /locus_tag="PKN55_14085"
     CDS             1691..>1888
                     /locus_tag="PKN55_14085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX72422.1"
                     /translation="MKHTPSIHFSLAFIVAPEGDNGRDGKFWLNADACVNKKSNLGSW
                     RLAAVHPRVARRASQKVREVSG"
BASE COUNT          279 a          611 c          592 g          406 t
ORIGIN      
        1 caccgtgacc ctgaccgagc gtgccgccgc aggcagaaca agtggtacgg cggggtcggg
       61 caacaccgcc aggcgggttt tgtgagctgc gctgaatacg cgctctcttc cgcctgcatg
      121 cctcatattt ctggagaacc ccgcatgtcc ccgattgcct ggccgcagcg tttgcgcgcc
      181 atcctgcaca aagaatggtt tttgtttggc atgctcggcg cggtggcgct ggccagtctg
      241 gcgccggaca tcggtcgcgc gggtggcccg ctgcacggcg atgtgttggc cgatcttggc
      301 attgccgtgg tgttttttct gcatggcctg gggatttcgt ttgccagcct gcgccagggc
      361 atgctgcgct ggccggtgca tctgctggtg cagttgctga cctttgccgt gtttccgctg
      421 ctgtggctgc tggccgcgcc gctggtgagc gcctggatgc cggccgggct ggcgctgggc
      481 ttttgctatc tgtgtgcctt gccgtccacc atctcgtcat cggtggcgat gaccgggctg
      541 gcgcgtggca atgtgccggc agcgattttc aacgccaccc tgtccagcgt gctgggcatt
      601 gcgctcaccc cgctgctggt ggcggcgctg gcccatctgg gcggtggcca gggcatggat
      661 ctgggccagg cggtgctcag cattgcccgc ctgctcttgc tgcccctgct ggccggacaa
      721 ctgctgcgcc cgctgctgca cgcctggcat cagcgttaca aacgctatac caatctgctg
      781 gaccgctggg tgattttgct gctggtgtac acggcattct gtgattcggt ggcctccggc
      841 ctgtggcgtg accatggcct tgccaccctg gccacggcca tgctgggtgc ggcgctggcg
      901 ctgctggtgg tgctgacgct gtccacctgg ctggcgcggc gcttgggctt cagtgtggaa
      961 gacgagatca ccacggtgtt ctgcggctcg aaaaaaaccc tggcctctgg tgtgccgatg
     1021 gccaaactgc tgtttggcgc ccacccggcc attggcctga tcgtgctgcc gatcatgttt
     1081 tatcaccaga tccagctgct ggtgtgcgcg gtgctggcca accgctacgc cgcccggcgt
     1141 tagcgcagcc cacaaaatcc tcctggcgtc gttacacgac cttgccgtac tgcttgtctg
     1201 ctggccagga tcgctgcgct ggattttgtt agccgctctt agcccgcaga cgcacgcccg
     1261 ccccggcgcc ggtaggcagc ggggcgggca acacagcagt ggctcacgcg ctcaggcgtc
     1321 ttcgcgcagc tcaaagtcgt gggtgatctc ggcggtcttg gccagcatga tcgacgccga
     1381 gcagtatttc tctgccgaca atttgatcgc ccgctccacc gcatccgcct tcaagccacg
     1441 gccggtaacg acaaaatgaa aatgaatgcg ggtgaacacc ttgggatcca cttccgcgcg
     1501 ctcggcatcg agctccaccc agcaatcccg cacatcctga cgcgacttgc gcaaaatgct
     1561 gagcacgtca taactggtgc acccggccgt gccaagcagc accaactcca tcggccgcgg
     1621 ccccagatta cgcccgccgc cctccggcgc gccgtccatc accacggcgt gaccgctgcc
     1681 ggtctcgccc atgaaacata ctccgtcaat ccatttcagc ttggctttca ttgtggctcc
     1741 tgagggggat aatggtcgtg atgggaaatt ctggctgaac gcggatgctt gcgtaaacaa
     1801 gaaatcaaac cttggttcgt ggcgcttagc cgctgttcac ccccgtgtgg cgcgccgagc
     1861 atcgcagaaa gtcagggagg tttccggc
//
LOCUS       DAOPYX010000421         1887 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_92299_length_1887_cov_2.465066, whole genome shotgun sequence.
ACCESSION   DAOPYX010000421 DAOPYX010000000
VERSION     DAOPYX010000421.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 1887)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1887)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1887
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92299_length_1887_cov_2.465066"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(78..728)
                     /locus_tag="PKN55_14090"
     CDS             complement(78..728)
                     /locus_tag="PKN55_14090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX72423.1"
                     /translation="MCSAVSSIGSAAVSFASECLPKVGTILTTLGGSLGMVANVVVAV
                     CQILSILRQDESAEDLGDRALQAAENGIRPENFDRYDDYLDSIRKFEVNPEKSAQYSP
                     DTKAAAGMQVAISSLEDHFKTPVGALYGLPILIAMNKDFFNPDRLSALLKTTHDINAV
                     VDYLDGRLGRSERATVETTLLKAEQSLTPEKNLADVKGDLREVAAKLEAQMNNVNT"
     gene            complement(816..>1887)
                     /locus_tag="PKN55_14095"
     CDS             complement(816..>1887)
                     /locus_tag="PKN55_14095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX72424.1"
                     /translation="DLTLGTKGRMLNTRQSLDFDQLLDQCVVIELEEIKDESDKALFM
                     GLILTRVAESIKHRHRRDPGFRHLTLLEEAHRLLSKPDPGEQGSKKLGVDMFCNLLAE
                     VRKYGEGLIIADQIPNKLVSDVIKNTNLKIVHRLFAADDRNVIGDTMCLSDEQKDYLP
                     ALQAGQAVIYGGGWHAPVHAQIVRNANTTDQPLDEALIRQHGRAQLWAQRHVLYPQLA
                     ALDILADGDQLAGFQREASKLVGMLLRVMVALSVRQSGQPKNMLPPEQLYPRLRDRVQ
                     HSMPAWRARLDDTSLLALLRACIYDETDIPLSTDHDAALNEWLTAVVLEPVADVSALR
                     ADVLEEARKDVENWVQRSWLAI"
BASE COUNT          379 a          582 c          501 g          425 t
ORIGIN      
        1 catttccaat attctttgcc gcataagcag cagcactaaa atctccgaca gatgaccaga
       61 aacccatttg actttcctca agtatttacg ttattcatct gtgcctccag cttggccgcc
      121 acctcgcgca gatctccctt cacatccgcc aggttttttt ccggcgtcag gctttgttcg
      181 gctttcagca aggtggtttc caccgtggcg cgttcactgc ggcccagccg gccatccaga
      241 taatccacga cggcattgat gtcatgcgtg gttttcagca gggcactcag ccggtcggga
      301 ttaaaaaaat ccttgttcat ggcaatcagg atcggcaggc catacaaagc gccaaccggg
      361 gtcttgaagt ggtcttccag gctgctgatc gccacctgca tgccggcggc agccttggtg
      421 tccgggctgt attgcgcgct tttttccggg ttgacttcaa acttacgaat actatccaga
      481 taatcgtcat aacggtcgaa gttttccggg cggataccgt tttccgccgc ttgcagggcg
      541 cggtcgccca gatcctcggc gctctcgtcc tggcgcaata tggacaggat ctggcatacc
      601 gccaccacga cattggccac catacccagc gagccaccca gtgttgtcag aatggtgccg
      661 acttttggca ggcattcgct ggcaaagctg acggctgcgc tgccaatgct ggatacagcg
      721 gaacacacgg cagaaagacc actggaaatt gcactcccta cactactcca aaatcccatt
      781 tcattttctc cttagaaata tcctgttcag tctgttcaaa tggccagcca gctacgttgc
      841 acccaatttt ccacatcctt gcgagcctct tccaagacat cggctcgcaa agcagataca
      901 tccgccaccg gctccagcac tacggctgtc agccattcat tcaaagccgc atcatggtca
      961 gtgctgagtg gaatatcggt ttcgtcatag atgcacgcac gtagcagcgc caatagcgaa
     1021 gtgtcgtcca ggcgtgcgcg ccatgccggc atgctgtgct gcacacgatc acgcaagcgc
     1081 ggatacagct gttcaggtgg cagcatgttt tttggctgcc cggattggcg caccgacaac
     1141 gccaccatca cccgcagcaa catccccacc agcttgctgg cctcccgctg aaacccggcc
     1201 agttggtcgc catcagccag aatatccagc gccgccagct ggggatacag cacatggcgc
     1261 tgcgcccaca gctgggcgcg gccatgctgg cgaatcagcg cctcatccag cggttgatcg
     1321 gtggtgttgg cgttgcggac gatctgcgca tgcactggcg cgtgccagcc gccgccgtaa
     1381 atcaccgcct ggccggcttg caacgcgggc aagtagtctt tctgctcatc gctcaggcac
     1441 atggtgtcgc caatcacatt gcggtcatcg gcagcaaaca ggcgatgaac aattttcagg
     1501 ttggtgttct tgatcacatc gctgaccagc ttgttgggaa tctggtcggc aatgatcagc
     1561 ccctcgccgt atttgcgcac ttcggccagc aggttgcaga acatgtccac ccccagcttc
     1621 ttgctgccct gctcgccggg gtcgggcttg ctcagcaggc gatgggcttc ttccagcaag
     1681 gtgaggtggc gaaacccagg atcacggcga tggcggtgct tgatgctctc ggccaccctg
     1741 gtgaggatca gccccataaa cagggctttg tcggattcgt ccttgatttc ttccagctcg
     1801 atcaccacgc actggtcgag cagctggtca aaatccagcg actggcgggt gttaagcatg
     1861 cggcccttgg tgcccagggt caggtcg
//
LOCUS       DAOPYX010000422         1874 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_93254_length_1874_cov_14.677295, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000422 DAOPYX010000000
VERSION     DAOPYX010000422.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 1874)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1874)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1874
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93254_length_1874_cov_14.677295"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(108..1850)
                     /gene="ptsP"
                     /locus_tag="PKN55_14100"
     CDS             complement(108..1850)
                     /gene="ptsP"
                     /locus_tag="PKN55_14100"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012698389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HNX72425.1"
                     /translation="MSISLHGIGIGGGIAIGRAHLISQSAADVAHYTLEAHELPAEKQ
                     RFETAIRATRKELEMLWGSIPENAPAELGAFLSLHIMLLNDATISREPLKVIDALRCN
                     AEWALKTQSDQLVAQFEDIEEQYLRERKQDVLQVVERIFKNLAGQSSVLPMPDPLNDD
                     AILIAHDLSPADMVYFKDSSYVAFVTDVGGATSHTAILARSLDLPSVIATHNARQLIS
                     EDEIVIVDGIQGVVIVAPDEQVLAEYKRRQRAWREARRKLSSIKKANAVTLDGAPIEL
                     VANIELPQDVEDVKAAGAVGIGLFRSEFLFLGRDTLPSEDEQFEAYRQVVADMKGFPV
                     TIRTMDLGADKNPKWSVHGVSDNPALGLCGIRLCLAEPQLFRSQLRALLRASTHGKLR
                     VLFPMINGLTDLKQALAQLDLARHELRGENIPFDEKLEVGAMIEIPSAALVINSLVKH
                     VNFFSVGTNDLIQYTLAIDRNDDAVSSLYDPTHPAVLYLIQHTIKAANKAGLPVSVCG
                     EMAGDARLTRLLLGMGLRKFSMHPANMLSVKQVVLNTHLDRLHTLTGRILRADDPDKI
                     HDLLQQLNALDCAA"
BASE COUNT          314 a          538 c          648 g          374 t
ORIGIN      
        1 gcttgagcgc ggcggggtcg atggcgtctt gcagcatgaa aacgataggg cctgtttgag
       61 gttgagggcg cgccgcccca atagggcggc cgcgccagcg gccagcctca ggcggcgcag
      121 tccagcgcgt taagctgctg gagcaagtcg tggattttat ccggatcgtc ggcgcgcagg
      181 atacggccgg tcagggtgtg caggcgatcc aggtgggtgt tgagcaccac ttgcttgacc
      241 gagagcatat tggccgggtg catggagaac ttgcgcaggc ccatgcccag cagcaggcgg
      301 gtgaggcggg cgtcgccggc catttcgccg cacaccgaca ccggcaggcc ggccttgttg
      361 gcggccttga tggtgtgctg aatcagatac agcaccgccg ggtgggtggg gtcgtacagc
      421 gagctgacgg cgtcgtcgtt gcggtcgatg gccagggtgt actgaatcag gtcgttggtg
      481 cccaccgaga aaaaattgac gtgcttgacc agcgagttga tcaccagcgc ggcggaggga
      541 atttcaatca tcgcgccgac ttcgagtttc tcgtcgaagg ggatgttctc gccgcgcagt
      601 tcgtggcggg cgaggtccag ctgggccaag gcctgcttga ggtcggtcag gccgttgatc
      661 atcgggaaca gcacgcgcag cttgccatgg gtggaggcgc gcagcagggc gcgcagctgc
      721 gagcggaaca gctgcggttc ggccaggcac aggcggatgc cgcacaggcc cagcgccggg
      781 ttgtccgaga cgccatgcac cgaccatttg gggtttttgt cagcgcccag gtccatggtg
      841 cggatggtga ccggaaagcc tttcatatcg gccaccacct ggcgataggc ttcgaattgc
      901 tcgtcttctg agggcaaggt gtcgcggccc aaaaacagaa actcgctgcg gaacaggcca
      961 atgcccaccg cgccagcggc tttgacgtct tcgacgtctt gcggcagttc gatattggcc
     1021 accagctcga tgggcgcgcc atccagggtg acggcattgg cttttttgat gctggagagc
     1081 ttgcgccgcg cttcgcgcca ggcgcgctgg cggcgtttgt attcggccag cacttgctcg
     1141 tccggcgcga caatcaccac gccctgaatg ccatcgacga tgacgatttc gtcttcgctg
     1201 atcagctggc gggcgttgtg ggtggcgatg accgagggca aatccaggct gcgcgccagg
     1261 atggcggtgt ggctggtggc gccgccgacg tcggtgacaa aggcgacgta gctggaatcc
     1321 ttgaaataca ccatgtcggc cggcgacagg tcatgggcaa tcaggatggc gtcgtcgttg
     1381 agcgggtcgg gcattggcag cacgctgctc tggccggcca ggttcttgaa gatgcgctcg
     1441 accacttgca gcacgtcctg cttgcgttcg cgcaggtatt gttcttcgat gtcttcaaac
     1501 tgggccacca gttgatcaga ctgggttttc agcgcccatt cggcgttgca gcgcaaagca
     1561 tcgatcacct tcagcggttc gcgcgagatg gtggcgtcgt tgagcagcat gatgtgcagc
     1621 gacaaaaacg cccccagctc ggccggcgcg ttttccggga tgctgcccca gagcatttcc
     1681 agctctttgc gggtggcgcg gatggcggtt tcaaagcgct gcttttccgc tggcagttcg
     1741 tgcgcttcca gcgtgtagtg cgccacatcg gcggcgcttt gcgaaatcag gtgcgcgcgt
     1801 ccgatggcga tgccgccgcc aatgccgatg ccgtgcagac tgatgctcat ggcgtttcct
     1861 ccccctgcga gact
//
LOCUS       DAOPYX010000423         1868 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_93688_length_1868_cov_7.409818, whole genome shotgun sequence.
ACCESSION   DAOPYX010000423 DAOPYX010000000
VERSION     DAOPYX010000423.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 1868)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1868)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1868
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93688_length_1868_cov_7.409818"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..811)
                     /locus_tag="PKN55_14105"
     CDS             complement(<1..811)
                     /locus_tag="PKN55_14105"
                     /inference="COORDINATES: protein motif:HMM:NF012573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX72426.1"
                     /translation="MIAEGSTMASTTGNTITLAANETDYSLASSTGFIIAGNALNNQL
                     TGNLGNDTLLGAAGADTLLGSDGNDSLDGGLGADWMAGGAGDDSYVVDDAGDVVVELA
                     NEGVDSITSKISLVLGDNIERLYAAQSGLSLTGNAMANFMRGSNGADALNGGDGNDTI
                     YTFATNSTVGGDDTVHGGNGNDIIYVGGNVTSYVYGDAGNDTIYLSYGAHPTYARGGT
                     GNDTYIITNALSQIEEFAGEGIDTIRTTVTMSLQPGEMVNPYSVPLISGEVE"
     gene            complement(1280..>1868)
                     /locus_tag="PKN55_14110"
     CDS             complement(1280..>1868)
                     /locus_tag="PKN55_14110"
                     /inference="COORDINATES: protein motif:HMM:NF025056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HNX72427.1"
                     /translation="EWHDVFLSHTRADREQAEQLAEQLRAAGLSVYLDVDDEALSRLH
                     GRELAERLKNILSRSRLLLFVFSARSTESRWMPWELGLAHGVVGRVLLWPLNAAAGRA
                     AARQEYLKLYEQVNAIHPLEDALARVYSARDAAVAPAHEHAAAALGEITGQQGLALAQ
                     PAHAWEFGWRGPWQLYMAWWQALWGTGSRAKSPHE"
BASE COUNT          358 a          551 c          538 g          421 t
ORIGIN      
        1 tttcgacttc gccggaaatc agcgggacgg aataaggatt caccatttct ccgggttgca
       61 aggacatagt gacggtggtg cggatggtgt caatgccttc gccggcaaat tcttctatct
      121 ggctcagagc attggtgatg atataagtgt cgttgcccgt gccgcctcgg gcataggtgg
      181 gatgagcgcc ataactcaga taaatcgtat cgttaccggc atcaccgtac acataggatg
      241 tgacattccc tcccacataa atgatgtcgt tgccattgcc accatgcacg gtatcgtcac
      301 cgcccaccgt ggagttggtg gcaaaggtgt aaatggtgtc attgccatcg ccgccattga
      361 gtgcatccgc tccgtttgag ccacgcatga aattggccat ggcattcccg gtcaagctca
      421 gaccagattg ggctgcatac agccgctcga tattgtcgcc cagcaccagg gatatcttgc
      481 tggtaatgct gtccaccccc tcgttggcca gctcaaccac cacatcgcca gcatcatcca
      541 ccacataact gtcatcaccg gcgccgccag ccatccagtc ggcgcccagg ccgccatcca
      601 gcgagtcatt gccgtcactg cccagcaagg tgtcagcacc tgctgcgccg agcagggtat
      661 cgttacccag attgccggtg agctggttgt tcagggcgtt gcccgcaatg atgaagccag
      721 tggagctggc cagcgagtaa tcggtttcgt ttgctgccag ggtgatggta ttgcctgtgg
      781 tgcttgccat ggtagagccc tccgcaatca atagatcatg agcgacacaa aaaatcctta
      841 aacttgcatt tttattctat gaatctgttt gattttattt caatcagtgt aaatactgct
      901 tttggcagca atatttatcc aagatcaagt gaagtgttgt ctgtgtaggg tctacttggt
      961 gtcgccttgc ttccatgcgt gcgtctggac gtgcatgctg attggtgatt gaaacacttt
     1021 tggcatgagt ctcacctgag ggagtgttcc tgcgcaggcg ggaatcacac ctcaggtgac
     1081 gcacggcgag tgctgcgtcg tggcgcatga gcccttgttt ttcaacgtgg ctggctacta
     1141 ggcgctgccc atcaacaccc tgaggtgtcc tgtttgctgt ccatgcaatc catgcgatgc
     1201 cgtgtttttg gcggctgtaa aaaaacccag cccccctgat aggctggggc tgggttttcg
     1261 gtgcacccgg tcgggtcgat tattcatgag gggatttcgc acggctgccc gtgccccata
     1321 gcgcctgcca ccaggccata tacagttgcc acgggccacg ccagccaaat tcccaggcgt
     1381 gcgctggctg cgccagcgcc aagccctgct ggccggtgat ttcacccagc gcggcggcgg
     1441 cgtgttcgtg cgccggggcc actgccgcat cgcgggcgct gtagacgcgc gccagcgcgt
     1501 cttccagcgg gtggatggca ttcacctgct cgtatagctt caggtattcc tggcgggcgg
     1561 cggcgcggcc ggcggcggcg ttcagcggcc acagcagcac gcggcccacc acgccgtgcg
     1621 ccaggcccag ctcccatggc atccagcgcg attcggtcga gcgcgccgaa aacacaaaca
     1681 gcaataaacg gctgcggctg aggatgtttt tcagccgctc ggccagctcg cggccatgca
     1741 ggcgcgacaa ggcttcgtcg tccacgtcca gatacacgct caagccagca gcgcgcagct
     1801 gttcggccag ttgctcggct tgctcgcggt cggcgcgggt gtgcgagaga aacacatcat
     1861 gccattcc
//
LOCUS       DAOPYX010000424         1863 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_94076_length_1863_cov_15.893805, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000424 DAOPYX010000000
VERSION     DAOPYX010000424.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 1863)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1863)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1863
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94076_length_1863_cov_15.893805"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            48..962
                     /locus_tag="PKN55_14115"
     CDS             48..962
                     /locus_tag="PKN55_14115"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HNX72428.1"
                     /translation="MTDLAAHTVMTARLSSFSAIPRTLLLAAGASLLLHLAVVLGVRF
                     VPPDPRAWLGDRTLDVVLVNSRHSEAPKKADALAQVNMNGAGNTIDPGLRARTPLPAT
                     SVQHEASRPSVAQSPRPSVAPTPPSESEVISAAKSRPAPQQAIARHTPQHPDKGLNPT
                     DLLAQAREIARLEGEIAEKTSALQSQPRRADFGGRTREYRFARYVEDWRLKIERVGNL
                     NYPEEARRNGIYGSLILSVEINADGSLKNIRVDRSSGHAELDDAARRIVKLAAPYASF
                     PEALKRDFDYLRIVRTWTFTRENQLAAE"
     gene            987..1364
                     /locus_tag="PKN55_14120"
     CDS             987..1364
                     /locus_tag="PKN55_14120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012698618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ"
                     /protein_id="HNX72429.1"
                     /translation="MYEVNRSIAIIKPKQPFFDWLNALPFEQDEALTLTALRQDCNAL
                     LIPPAEDFEDARDFIRTRWRGLFEAELADWCDDDTLWPEKLTPNLFQQWFDVEIHSVL
                     TDLVEEPLEREQFAELDLSQDED"
     gene            1508..>1863
                     /locus_tag="PKN55_14125"
     CDS             1508..>1863
                     /locus_tag="PKN55_14125"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012698617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbgC/FadM family acyl-CoA thioesterase"
                     /protein_id="HNX72430.1"
                     /translation="MHHTPIKVRGYHLDLYGHVNNARYLEFLEEARWAYFEATHGLEW
                     FTESGLAFVVVNININYRRPAFMNEQLVVHTGVKSLSNRSAVIHQRITLDDMSTVVSE
                     ADITFVLFDEKQNKAIV"
BASE COUNT          378 a          623 c          531 g          331 t
ORIGIN      
        1 ccacgcacca agcaaccccc accctacccc gtgttacact tgccagcatg accgacttgg
       61 ctgcccacac cgtcatgact gcccggcttt cttctttttc tgccataccc cgcaccctgc
      121 tgctggcggc tggcgcttcg ctgctgctgc acctggccgt ggtgcttggc gtgcgctttg
      181 tgccgcccga tccgcgcgcc tggctgggcg accgcacgct ggatgtggtg ctggtcaaca
      241 gccggcacag cgaggcgcca aaaaaagccg atgcgctggc gcaggtgaac atgaacggcg
      301 ccggcaacac catcgaccct ggcctgcgcg cgcgcacgcc gctgccggcc acctcggtgc
      361 agcatgaagc cagccggcca agcgtggcgc aaagcccgcg cccgtcggtg gcgcccacgc
      421 cgcccagcga aagcgaagtg atttccgccg ccaagagccg gccggcgccg cagcaggcaa
      481 ttgcccgcca caccccgcag cacccggaca aggggctgaa ccccaccgac ctgttggccc
      541 aggcgcgcga gattgcccgg ctggaaggcg aaatcgccga aaaaaccagc gcgctgcaat
      601 cgcaaccgcg ccgcgccgat tttggcggac gcacccgcga ataccgtttt gcccgctatg
      661 tggaagactg gcggctgaaa atcgaacgcg tcggcaacct gaattacccg gaagaagccc
      721 gccgcaacgg gatttatggc agtctgatcc tcagcgtgga gatcaacgcc gacggcagcc
      781 tgaaaaatat tcgcgtggat cgcagctctg gccatgccga gctggacgac gccgcccggc
      841 gcattgtcaa actggccgcg ccgtatgcgt ctttccctga ggcgctaaag cgcgatttcg
      901 attatctgcg cattgtgcgc acctggactt ttacccgcga gaaccagctc gctgcagaat
      961 aaacttgccg atttggagac ccgcccatgt atgaagtcaa ccgcagcatc gccatcatca
     1021 aacccaagca gccgtttttc gactggctca acgccctgcc cttcgagcag gacgaagcgc
     1081 tgacgctgac cgcgctgcgc caggactgca acgccctgct gattccgccg gcagaagact
     1141 ttgaagacgc ccgcgatttt atccgcaccc gctggcgcgg cctgtttgaa gccgaactgg
     1201 ccgactggtg cgacgatgac accctgtggc cggaaaagct cacccccaat ctgttccagc
     1261 aatggtttga cgtggaaatc cactcggtgc tgaccgacct ggtggaagag ccgctggagc
     1321 gcgagcagtt tgccgagctg gatttgagcc aggacgaaga ctgattggcg gggtagggcg
     1381 cataaaaacc gggagcataa gcaaacactt gtttaaactc cctgcatcgc gctatcttca
     1441 tgacaggcgg gccgtggccc gcctgttttg acatcattcg ccggtttatt ttctggagcc
     1501 tccccccatg catcacaccc cgatcaaggt tcgcggctat cacctggacc tctacggcca
     1561 cgtcaacaac gcccgctatc tggagtttct ggaagaagcg cgctgggcct actttgaagc
     1621 cacccacggc ctggaatggt tcaccgaatc cggcctggcc tttgtcgtgg tcaatatcaa
     1681 catcaactac cgccgcccgg ccttcatgaa cgagcagctg gtggtgcaca ccggggtgaa
     1741 atcgctgtcc aaccgcagcg cggtgatcca ccagcgcatc accctggacg atatgagcac
     1801 ggtggtgtcg gaagccgaca tcacctttgt gctgttcgac gagaagcaaa acaaagccat
     1861 tgt
//
LOCUS       DAOPYX010000425         1856 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_94610_length_1856_cov_11.292060, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000425 DAOPYX010000000
VERSION     DAOPYX010000425.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 1856)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1856)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1856
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94610_length_1856_cov_11.292060"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            76..>1856
                     /locus_tag="PKN55_14130"
     CDS             76..>1856
                     /locus_tag="PKN55_14130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012698400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HNX72431.1"
                     /translation="MLTRILLGFLVVCLALAIIAAGLLGFAVAVTYPKLPSLEVLTDY
                     RPKIPLRIYTADGVQIGEFGEERRAFTRIQDVPQLMKQAIMAAEDERFYQHGGIDYVG
                     VARAMLGNVVSGHPKSGASTITMQVARNFFLSSEKTLTRKFNEALLAFKIEHNLSKDQ
                     ILELYFNQIYLGQRAYGFAAAGQAYYGRPLKELSIAQMAMLAGLPKAPSSYNPVVNPD
                     RARLRQLYVLRRMRELNFISEAQYEAAKNEPNSVSRQSEDFSVPAQYVAEMVRQTMFE
                     RYKENAYTDGYRVYTTVDSQHQKWAFDALRAGLTEYDVRHGYRGPESFIDLKTLPAGE
                     RDESLDESLAAIRDSGKLQPGVVLEASANKVVVYMRGGRQVEVSGAGLNFARRALSPK
                     LTPAQQIRPGAVVRVQQNDKGSWEIGQMPQVEGAFVSMDPHDGAIKSLVGGFDFNRNH
                     FNRVTMAWRQPGSSFKPFIYSAGLERGFTPSSMINDAPLVIDPQSIGGQRWEPKNYDG
                     KFGGMMTMRQALTRSKNLVSIRILMAIGVDYAQEYIGHFGFGPKQHPAYLTMALGAGV
                     VTPLQMAEGYSVFANGGSRVRSYLIDR"
BASE COUNT          359 a          563 c          561 g          373 t
ORIGIN      
        1 gctgtctgcc ttgcgcagac agcggcgcgg gcgaaggtct ataatcgccc atccttcatt
       61 cttgggccta tgctcatgct gacccgtatc ctgcttggtt ttctggtggt gtgtctggcg
      121 ctggccatca ttgccgccgg cctgcttggc tttgcggtgg ccgtcaccta tcccaaactc
      181 cccagcctgg aagtgctgac cgactaccgg ccgaaaatcc ccttgcgcat ctacaccgcc
      241 gacggcgtgc agattggcga atttggcgaa gaacgccggg ctttcacccg catccaggat
      301 gtgccgcaac tgatgaagca ggccatcatg gccgccgagg acgagcgttt ttaccagcat
      361 ggcggcattg attatgtcgg tgtggcgcgg gccatgctgg gcaatgtggt gtcgggccat
      421 cccaaatccg gagcctccac catcaccatg caggtggcgc gcaatttctt cctgtcttca
      481 gaaaaaaccc ttacccgcaa attcaacgaa gccttgctgg cgttcaagat cgaacacaac
      541 ctcagcaagg atcagattct tgagctgtat ttcaatcaga tctatctggg ccagcgcgcc
      601 tatggttttg ccgctgccgg ccaggcgtat tacggccgcc cgctgaaaga actgtcgatt
      661 gcccagatgg ccatgctggc cggtctgccc aaggcgccat cgtcgtataa cccggtggtc
      721 aacccggatc gcgcccgtct gcgccagctg tatgtgctgc gtcgcatgcg cgagttgaat
      781 ttcatcagcg aagcgcaata cgaagccgcc aagaacgagc ccaatagcgt gtcgcgccag
      841 agcgaggatt tcagcgtgcc ggcgcagtat gtggccgaga tggtgcgtca gaccatgttc
      901 gagcgctaca aggaaaacgc ctacaccgat ggctaccggg tgtacaccac ggtggacagt
      961 caacaccaga aatgggcatt cgacgccctg cgcgccggcc tgaccgaata cgatgtgcgc
     1021 cacggctatc gcggcccgga atcgtttatc gacctgaaaa ccctgccggc tggcgagcgc
     1081 gacgaatcgc tggatgaatc gctggccgcc atccgcgaca gcggcaagct gcaaccgggc
     1141 gtggtgctgg aagccagcgc caataaggtg gtggtgtata tgcgcggcgg ccgtcaggtg
     1201 gaagtcagcg gcgccgggct gaattttgcc cgccgtgcgc tgtcgcccaa gctcactcct
     1261 gcccagcaga ttcgtcccgg cgcggtggtg cgcgtgcaac agaacgacaa gggaagctgg
     1321 gaaatcggcc agatgccgca ggtggaaggc gcgtttgtgt cgatggaccc acacgatggc
     1381 gccatcaagt cgctggtggg cggctttgac tttaaccgca accacttcaa ccgcgtgacc
     1441 atggcctggc gtcagcctgg ctccagcttc aagccgttca tttattcggc cggcctggaa
     1501 cgcggcttca cgccgtcgtc gatgatcaac gatgcgccgc tggtgattga tccgcaatcc
     1561 atcggcgggc agcgctggga gccgaaaaac tacgacggca aatttggcgg catgatgacc
     1621 atgcgccagg cgctgacccg ctcgaagaac ctggtgtcca tccgtatcct gatggccatc
     1681 ggcgtggact acgcacagga atatatcggg cattttggtt ttggccccaa gcaacatcca
     1741 gcctatctga ccatggcgct gggcgctggt gtggtgacgc ccttgcagat ggccgagggc
     1801 tactcggtgt ttgccaatgg cggctcgcgc gtgcgctctt atctgatcga ccgcat
//
LOCUS       DAOPYX010000426         1852 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_94935_length_1852_cov_10.929327, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000426 DAOPYX010000000
VERSION     DAOPYX010000426.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 1852)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1852)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1852
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94935_length_1852_cov_10.929327"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(257..1507)
                     /gene="murA"
                     /locus_tag="PKN55_14135"
     CDS             complement(257..1507)
                     /gene="murA"
                     /locus_tag="PKN55_14135"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003773889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HNX72432.1"
                     /translation="MEKLKIVGNGPLNGDITVSGAKNAALPILCASLLTADTLRLTNV
                     PQLRDVLTTQKLLQGMGARVMTDNVHEYELTASDIHTLQAPYELVKTMRASILVLGPT
                     LARFGEASVSLPGGCAIGSRPVDQHIKGLAAMGADIVIEHGYVKARGKLKGARVVMDM
                     VTVTGTENLLMAATLAEGVTILENAAREPEVTDLAECLVKMGAKISGIGSDRLVIEGV
                     ERLHGAEHAVMPDRIEAGTFLVAAAVTQGHVVLRRAHAASMEAVLDKLVEAGCVVEAG
                     DDWISLDMKRRPKAVNVRTLPYPAFPTDMQAQFMTLNALAEGTSRVTETIFENRFMHV
                     PELNRMGANIATEGNTAIITGVPRLSGATVMATDLRASASLVIAGLVADGETIVDRIY
                     HLDRGYEHIEQKLAGVGAQIERIS"
     gene            complement(1590..1835)
                     /locus_tag="PKN55_14140"
     CDS             complement(1590..1835)
                     /locus_tag="PKN55_14140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA/IbaG family iron-sulfur metabolism protein"
                     /protein_id="HNX72433.1"
                     /translation="MLTPEQVKTLITAELDCTHVDVEGDGHHFFAVIVSPAFAGKRLI
                     ERHRLVMDIARAKLDSNELHALSITQALTPEEWAQKA"
BASE COUNT          332 a          574 c          591 g          355 t
ORIGIN      
        1 taaaaccaat caacaggggg cgcaggcggc ggctggccag agaaggagta ggcatggaca
       61 agaaaagccg ataaaaacaa tcgattataa cgcaattcga ctggtatatt gacttcagtc
      121 aaaccttacg cagattctgg cgtccgcgct cagggcgagg gcggccggca aatcagtaaa
      181 gcgctcctgg cgaggcgcac ggccgcagac agcgccagca ccgtacggca agccaggagg
      241 gggtgatgcg cggcttttag ctgatgcgct cgatttgcgc accgaccccg gccagctttt
      301 gctcgatatg ctcgtagccg cgatccagat ggtaaatccg gtcaacgatg gtttcgccat
      361 ccgccaccag gccggcaatc accaggctgg ccgaggcgcg caggtcggtg gccatcaccg
      421 tggcgcccga caggcgcgga acgccagtga tgatggcggt gttgccctcg gtggcgatgt
      481 tggcgcccat gcggttcagc tcgggcacgt gcatgaagcg gttttcaaaa atggtttcgg
      541 tgacgcggct ggtcccttcg gccagggcgt tcaaggtcat gaactgcgcc tgcatatcgg
      601 tggggaaggc cgggtagggc agggtgcgca cattcaccgc tttagggcgg cgcttcatgt
      661 ccagcgaaat ccagtcgtcg ccggcctcca ccacgcagcc ggcttccacc agcttgtcca
      721 gcacggcttc catgctggcg gcgtgggcgc ggcgcagcac cacatggccc tgggtgacgg
      781 cggcggccac caggaaggtg ccggcttcga tgcggtctgg catcactgcg tgttcggcgc
      841 cgtgcaggcg ctcgacgcct tcgatcacca ggcggtcgct gccgatgccg ctgatcttgg
      901 cgcccatttt caccaggcac tcggccaggt cggtgacttc cggctcgcgc gcggcgtttt
      961 ccagaatggt gacgccctcg gccagggtgg cggccatcag caggttttcg gtgccggtga
     1021 cggtgaccat gtccatcacc acgcgcgcgc ccttgagctt gccgcgcgcc ttgacgtagc
     1081 catgctcgat gacaatatcc gcccccatcg ccgccaggcc cttgatgtgc tgatccaccg
     1141 gccggctgcc aatggcgcaa ccgcccggca gcgacacact ggcctcgcca aagcgcgcca
     1201 gcgtcgggcc cagcaccaga atcgaggcgc gcatggtttt caccagctca tacggcgctt
     1261 gcagggtgtg gatgtcgctg gcggtgagtt cgtattcgtg gacattgtcg gtcatcaccc
     1321 gcgcgcccat gccttgcagc agcttttgcg tggtcagcac atcgcgcagc tgcggcacat
     1381 tggtcagccg cagggtgtcg gcggtcagca ggctggcgca caaaatcggc agcgccgcat
     1441 ttttggcgcc cgacacggtg atgtcgccgt tcagcgggcc attgccgaca attttcagtt
     1501 tttccatgac ttcaattcag cctttacaaa gggcaacggc gcgaaataat cgcgccggaa
     1561 acaagcgtgg tgacggtgtg gcgcagcgct caggcttttt gcgcccactc ttctggcgtc
     1621 agcgcctggg taatcgacag cgcgtgcagc tcgttgctgt ccagcttggc gcgggcgatg
     1681 tccatcacca gacgatggcg ttcgatcagg cgcttgcccg caaaggccgg cgagacaatc
     1741 accgcaaaaa agtgatggcc atcgccttcc acgtccacgt gggtgcagtc cagctcagcg
     1801 gtaatcaggg tttttacctg ttcgggggtc agcatcagca gtctcttttc tt
//
LOCUS       DAOPYX010000427         1841 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_95845_length_1841_cov_11.090146, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000427 DAOPYX010000000
VERSION     DAOPYX010000427.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 1841)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1841)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1841
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95845_length_1841_cov_11.090146"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..314)
                     /locus_tag="PKN55_14145"
     CDS             complement(<1..314)
                     /locus_tag="PKN55_14145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX72434.1"
                     /translation="MTPYSLRRSADYPAGNILLDTRFADKTVPLLLSHQPDALVAANL
                     LPASDTVRLTHLNNLFSVTRDLGTTLGAVRAGETLLIVCTNARIYHAALKWLNVREDS
                     AN"
     gene            432..773
                     /locus_tag="PKN55_14150"
     CDS             432..773
                     /locus_tag="PKN55_14150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019922527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HNX72435.1"
                     /translation="MSTLPPCPKCASEYTYEDGVQLVCPECGHEWSADAPADAGDADK
                     VYKDSVGNVLQDGDTVTVIKDLKVKGSSLVVKVGTKVKNIRLCDGDHDIDCKIDGIGA
                     MKLKSEFVRKV"
     gene            complement(888..1748)
                     /locus_tag="PKN55_14155"
     CDS             complement(888..1748)
                     /locus_tag="PKN55_14155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX72436.1"
                     /translation="MAHRPAALLALTLLAASTSALAADWSDTSIGYRYGTHFAEPFNQ
                     KDISKSIINLTHASGYKYGSNFFNADLLLADKNDPASVGSNTGSQEVYVVYRHTLDLG
                     KVTGADLKFGPVRGLGLTAGFDINTKDDAGYNSKKRMWVAGPTVMFDVPGFLNLSLLQ
                     LWESNAPYNGYSKTSVERYNYDPHPMLNLAWGIPFSIGSLPLSFEGYANFIAAKGKNE
                     FGVGTKPETNIDMQVMYDLSPLVNAQKKTFRVGLEYQYWRNKFGNDHKGAAGKGAFAK
                     TPMIRAEYHF"
BASE COUNT          329 a          501 c          630 g          381 t
ORIGIN      
        1 tcgttggcgc tgtcctcgcg cacattcagc catttcagcg cggcgtggta aatcctggcg
       61 ttggtgcaga caatcagcag ggtttcgccc gcgcgcaccg cgcccagcgt ggtgcccagg
      121 tcgcgggtga cgctgaacag attgttcagg tgagtcagcc gcacggtgtc gctggctggc
      181 agcagattgg ccgccaccag cgcatcgggt tgatggctga gcaagagcgg cacggttttg
      241 tcggcaaagc gggtgtccag caggatattg cccgcagggt agtcggcgct gcggcgcagg
      301 ctgtaagggg tcatcggggc gctcgcacaa agtggggatg ccctgatgca gcaagattcg
      361 cgccgtgacg acagaggcgg caaaaccctt gtacaatcac gggtttttcc ttttgccttg
      421 aaagaattgt catgagcacg ctgcctccct gtccgaaatg cgcctctgaa tacacctacg
      481 aagacggcgt gcagctggtc tgcccggaat gcggccacga atggtcggcc gacgccccgg
      541 cggacgcggg cgacgccgac aaggtgtaca aggattcggt gggcaatgtc ctgcaagacg
      601 gcgacacggt gacggtgatc aaagacctga aggtcaaggg ctcgtcgctg gtggtgaaag
      661 tgggcaccaa ggtgaagaac atccgcctgt gcgacggcga ccacgatatc gactgcaaaa
      721 tcgacggcat cggcgcgatg aagctgaagt cggagtttgt gcgcaaggtg tgatggcggg
      781 cgcatggctt gcgtgggttg gccgacgcaa gccggggcgc ggcgccggat gcccgcctgc
      841 gcgggcatga ccggctgagg tggcgacggt gagccgccat ccccccatca gaagtgatat
      901 tccgcccgga tcatcggcgt tttggcgaat gcgcccttgc cggcggcgcc cttgtggtcg
      961 ttgccaaact tattgcgcca gtattggtat tccaggccga cgcggaaggt ttttttctgg
     1021 gcgttgacca gcgggctgag gtcgtacatt acctgcatgt cgatattggt ttcgggcttg
     1081 gtgcccacgc caaattcgtt tttgcccttg gcggcgataa agttggcgta gccttcaaac
     1141 gacagcggca gcgagccgat ggagaacgga atgccccagg ccaggttcag catcgggtgc
     1201 gggtcgtagt tgtagcgctc gacgctggtc ttgctatagc cgttgtacgg ggcgttgctt
     1261 tcccacagtt gcagcaggct gaggtttaaa aagcccggca cgtcaaacat caccgtcggg
     1321 ccagccaccc acatgcgttt tttcgagttg tagccggcgt cgtccttggt gttgatgtca
     1381 aaaccggcgg tcaggcccag gccgcgcacc gggccgaatt tcaggtcggc gcccgtcacc
     1441 ttgcccaggt ccagggtgtg gcggtacacc acatacactt cttgcgagcc ggtgttggag
     1501 ccgacgctgg ccgggtcgtt tttgtcggcc agcagcaggt cggcgttgaa gaagttgctg
     1561 ccatacttgt aaccgctggc gtgggtcagg ttgatgatgc tcttgctgat gtctttttgg
     1621 ttgaacggct cggcaaagtg cgtgccgtag cggtagccaa tagaggtgtc gctccagtcg
     1681 gcggccaggg cggaggtgct ggcggccagc agagtgaggg caagcagcgc ggcgggacga
     1741 tgggccatga tgtgggttct ccaggaagtg cagaaatcaa gggtgtgcta atcgttgttc
     1801 gcgccttgtg ctggcgctta ttctcgaccc cgctgccgta t
//
LOCUS       DAOPYX010000428         1826 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_97129_length_1826_cov_4.896669, whole genome shotgun sequence.
ACCESSION   DAOPYX010000428 DAOPYX010000000
VERSION     DAOPYX010000428.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 1826)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1826)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1826
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97129_length_1826_cov_4.896669"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..417
                     /locus_tag="PKN55_14160"
     CDS             <1..417
                     /locus_tag="PKN55_14160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX72437.1"
                     /translation="LTHCLNMDALLRIRSRSESFKSGKNSPWHTDQEAMMRKSAVRGM
                     APYLPYREAMAQAMAVAVEDDEQEGGDGAGDSQPQAPALPEYTDTQMAENLPKWQAAI
                     DSGRTTPERIIALVSSKYTLTGDQETHILNMKKQGD"
     gene            419..>1826
                     /locus_tag="PKN55_14165"
     CDS             419..>1826
                     /locus_tag="PKN55_14165"
                     /inference="COORDINATES: protein motif:HMM:TIGR03033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaJ viral recombinase family protein"
                     /protein_id="HNX72438.1"
                     /translation="MQEQKIIQGTPEWHEFRAQHLNASDAPAMMGESPYKTRDALLRE
                     KAVGVVAEVSSETQRRFDDGHRFEALARPLAEKIIGEELYPVVGSEGKFAASFDGMTM
                     LGDVIFEHKTLNAALAAIDPDEELPLYYRIQMEHQFMVSGAEKCLFMASKWDAADQLI
                     GESHRWHSPDLALRQRIVDGWAQFEKDLAGYVPPEAKPEAVGRAPDALPALRIELTGQ
                     VTTSNLAEFRAHAQAVLGGIKKDNFQTDEDFASADKTAKWCSDVESRLDAAKQHALAQ
                     TTSIDELFRTLDAIKEDTRQVRLGLEKTVKARKEAVKLERINKAKSDFAAHVAALQSE
                     IADVRLTVLAPDFGGAIKGLKTLASIDNAIGVAMANAKIAADQRAADVRAKLAWFNEN
                     ATEYRSLFSDLQQLIGWPFDGFKQAVTARIVLRKQAEEAKLEAERERIRNEEAAKLAA
                     QAESIKPPTLAAADLAASL"
BASE COUNT          410 a          508 c          603 g          305 t
ORIGIN      
        1 ctgacgcatt gcttgaatat ggacgcgctg ctgcgcatcc gctcacgcag cgagtcattc
       61 aaaagcggga aaaacagccc atggcatacc gaccaggagg ccatgatgcg caaaagcgcg
      121 gtgcgaggaa tggcgccgta tctgccttac cgtgaagcca tggcgcaggc catggcggtg
      181 gcggtggagg atgacgagca agagggcggc gatggggccg gcgacagcca gccgcaggcg
      241 ccggccctgc ccgagtacac cgacacccag atggccgaaa acctgccgaa gtggcaggcg
      301 gcaatcgact ccggccggac gacgccggag cggattattg cgctggtgtc cagcaagtac
      361 acgctgaccg gcgatcagga aacacacatt ttgaacatga aaaagcaagg ggactgagat
      421 gcaggagcaa aaaattattc agggcacgcc ggaatggcac gagtttcgtg cccagcatct
      481 caacgcgagc gatgcgccgg cgatgatggg ggaatctccg tataaaaccc gtgatgcact
      541 actgcgtgag aaggcggttg gcgttgtcgc tgaggtgtcc agcgaaaccc agcggcgatt
      601 tgatgatggc caccgctttg aggcgctggc gcggccgctc gctgaaaaaa tcatcggcga
      661 agagctgtac ccggtggtcg ggtcggaggg caagttcgcc gccagctttg atggcatgac
      721 gatgctgggc gacgtgattt ttgagcacaa aaccctgaac gccgcgctgg cggccatcga
      781 tcccgatgaa gagctgccgc tgtattaccg catccagatg gagcatcagt tcatggtgtc
      841 cggcgctgaa aagtgcctgt ttatggcgtc aaagtgggac gcggctgatc agctgattgg
      901 ggagtctcac cgctggcact cccctgacct ggcgttgcgt cagcgtattg ttgatggctg
      961 ggcgcaattc gaaaaagacc tggcgggcta tgtgccgccc gaggccaagc ccgaggctgt
     1021 tggccgcgcg ccggacgcgc tgccggcgct gcgcattgag ctgaccgggc aggtaacaac
     1081 gtccaacctg gccgaattcc gggcgcacgc ccaggcggtg ctgggcggaa tcaagaagga
     1141 caatttccaa accgacgagg attttgcgag cgccgacaaa acagcgaagt ggtgctccga
     1201 cgtcgagtcg cggcttgatg ccgccaaaca gcatgcactg gcgcaaacga ccagcatcga
     1261 tgagcttttc cggacgcttg acgctatcaa ggaagatacg cgccaggtgc gcctggggct
     1321 ggaaaagacg gtcaaggccc gcaaagaagc cgtgaagctg gagcgcatca acaaggcgaa
     1381 aagcgatttt gccgcacacg tcgcggcgct gcaatcagag attgcagacg tccgcctgac
     1441 ggtgctggcg cctgattttg gtggagccat caaagggctg aaaaccctgg ccagcattga
     1501 caacgccatc ggcgtggcca tggcaaacgc caaaatcgcc gccgaccagc gggccgccga
     1561 tgtgcgcgcg aaactggcgt ggttcaacga gaacgccact gaataccgat ctcttttttc
     1621 cgatctgcaa cagcttattg ggtggccttt cgatgggttc aagcaggcag tgacggcgcg
     1681 tattgtcctg cgcaagcaag ctgaagaggc caagttggag gccgagcgcg agcgcatccg
     1741 caatgaagag gcggcgaagt tggcggcgca ggcggaaagc atcaagccgc cgacgttggc
     1801 tgctgctgac ctagcggctt cgctgg
//
LOCUS       DAOPYX010000429         1817 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_97909_length_1817_cov_8.679909, whole genome shotgun sequence.
ACCESSION   DAOPYX010000429 DAOPYX010000000
VERSION     DAOPYX010000429.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 1817)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1817)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1817
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97909_length_1817_cov_8.679909"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            151..1152
                     /locus_tag="PKN55_14170"
     CDS             151..1152
                     /locus_tag="PKN55_14170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008953865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HNX72439.1"
                     /translation="MPTRLPHLLATLCLTIGLAAPSWADATLLNVSYDVTRDFYKDYN
                     PAFQQYWKAKTGETVTLQQSHGGSSKQAMSVVAGLEADVITMNQAPDIDILAERGGLV
                     PAEWQKRLPDRSSPFVSTIVFIVRQGNPKAIQDWADLAKPGVQVIVPNPKTSGNGRYT
                     YLAAWGQAQQAGNAEQARAYVQKLFANVPVLDAGGRAATTTFMQRQIGDVLVTFENEA
                     EMVAREFGRGSFTVVYPKRSILAENPVSVVDKVVDKKGTRKVAQAYLEYLWSPAGQEL
                     AAQNYLRPRNPETLKKYAAQFPPIQTFTVEQVFGGWRKAMDTHFRDGGVFDQIYSKK"
     gene            1169..>1817
                     /locus_tag="PKN55_14175"
     CDS             1169..>1817
                     /locus_tag="PKN55_14175"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNX72440.1"
                     /translation="MTNRAAHFVDWLNQRFIQQGAPVQFSHEGPRVAAHGFGVALESA
                     FQPIIGAVSGRTLGWEGLVRPHSLAGESLPVVEFLDRFATGDDMARVDRICRSLHLAN
                     FVRATPADGYLFLNLHPRHLLSTRRHGEVFSHIADAAGVPPQRIVMEILEHKTSDEAQ
                     LADAVKALKARGFLIALDDFGQEGHSVHYDRLTALKPNIVKFDRSLIAGASEARRY"
BASE COUNT          361 a          589 c          558 g          309 t
ORIGIN      
        1 agcacagggc agtcagcccg cagggctgac cgcgccaccc gggtcgcctt tcttttcccc
       61 cttttctttg gcgagacaaa gaaaaggggt cgggtgcggg gcgaagcccc gcccaaaatc
      121 cagccccagc caatccacca ggagcccacc atgcccactc gtctgcccca cctgctcgcc
      181 accctgtgtc tgaccatcgg cctggccgcg cccagctggg ccgacgccac cctgctgaat
      241 gtcagctatg atgtcacacg ggatttttat aaggattaca atcctgcctt tcagcaatac
      301 tggaaagcca agaccggcga aaccgtcacc ctgcaacaat cgcatggcgg ctccagcaaa
      361 caggccatga gcgtggtggc cggcctggaa gcggatgtca tcaccatgaa ccaggccccg
      421 gacatcgaca tcctggccga acgcggcggg ctggtgccgg ccgaatggca aaagcgcctg
      481 ccagaccgca gctcgccgtt tgtctcgacc attgtgttca tcgtccgcca gggcaacccc
      541 aaggccattc aagactgggc cgacctggcc aagccgggtg tgcaggtgat cgtgcccaac
      601 ccgaaaacca gcggcaacgg ccgttacacc tatctggcgg cctggggtca ggcgcagcaa
      661 gcgggcaatg ccgagcaggc gcgcgcctat gtgcaaaagc tgttcgccaa tgtgccggtg
      721 ctggatgctg gcgggcgggc cgccaccacc accttcatgc agcgccagat tggcgacgtg
      781 ctggtgacgt ttgaaaacga agccgaaatg gtcgcccgcg agtttggccg cggcagcttt
      841 accgtggtct accccaagcg ctcgatcctg gcggaaaacc cggtcagcgt ggtggacaag
      901 gtggtggaca agaaaggcac gcgcaaggtg gcgcaagcct atctggaata tctgtggagc
      961 ccggctggcc aggaactggc tgcgcagaac tatttgcgcc cgcgcaaccc ggagactctg
     1021 aaaaaatatg cggcgcaatt cccgccgatc cagaccttca ccgtcgagca ggtgtttggc
     1081 ggctggcgca aggcgatgga cacccatttc cgcgatggcg gggtgtttga ccaaatttac
     1141 agtaaaaaat aaatttaagg atgtagtcat gacgaatcgc gcggcgcact ttgtggactg
     1201 gctgaaccag cgctttatcc agcaaggcgc gcccgtgcaa ttcagccacg aaggcccacg
     1261 cgtggccgcg cacggctttg gcgtggcgct ggagtctgcg ttccagccca tcatcggcgc
     1321 ggtgtccggg cgcacgctgg gctgggaagg cctggtgcgc ccgcacagcc tggctggcga
     1381 atcgctgccg gtggtggagt ttctcgaccg cttcgccacc ggcgacgaca tggcgcgggt
     1441 ggaccgcatc tgccgcagcc tgcacctggc caactttgtg cgcgccaccc cggcggatgg
     1501 ctatctgttt ctcaacctgc acccgcgcca cctgctgagc acccgccgcc atggcgaagt
     1561 gttctcgcac atcgccgacg cagccggcgt gccgccgcag cgcatcgtca tggaaattct
     1621 ggagcacaaa acctcagacg aagcgcaact ggcggatgcg gtgaaggcgc tgaaggcgcg
     1681 cggctttttg attgcgctgg acgactttgg ccaggaaggc catagcgtgc actacgaccg
     1741 cctgactgcg ctcaagccaa acatcgtcaa attcgaccgc agcctgattg ccggcgccag
     1801 cgaggcgcgc cgctatc
//
LOCUS       DAOPYX010000430         1811 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_98394_length_1811_cov_8.606492, whole genome shotgun sequence.
ACCESSION   DAOPYX010000430 DAOPYX010000000
VERSION     DAOPYX010000430.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 1811)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1811)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1811
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98394_length_1811_cov_8.606492"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..420
                     /gene="narH"
                     /locus_tag="PKN55_14180"
     CDS             <1..420
                     /gene="narH"
                     /locus_tag="PKN55_14180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008953007.1"
                     /note="with NarGJI catalyzes the reduction of nitrate; the
                     beta subunit is an iron sulfur cluster containing electron
                     transfer subunit; one of 3 nitrate reductases in E. coli
                     and in E. coli is expressed when nitrate levels are high;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HNX72441.1"
                     /translation="SVKDLRIPIQYLANLLTAGKEEPVIKALETMVAMRRYMRKKSVD
                     KVVDPNTLKGTHLNPAQVEDMYQVMAIANYEDRFVIPSSHKEMVENAFEDKASCGFTF
                     GNGCSDGHSNESLFGRKKATPIVFHDMRRDRARQAGE"
     gene            425..1090
                     /gene="narJ"
                     /locus_tag="PKN55_14185"
     CDS             425..1090
                     /gene="narJ"
                     /locus_tag="PKN55_14185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003784483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HNX72442.1"
                     /translation="MSTPTHPLSYRVLSLLLDYPEQPLIDALPELDSALADVEPALCA
                     QLAPLFTHLGGEPSLIALQETYVGTFDRQPAHSLHLFEHLHGEDRARGQAMVDLIEEY
                     RAHGFEPVSHELPDYLPLFLEFLSLCAADEARRLLDDAVHVVAHIGRQLASRGSPYAC
                     VLQALAALASVEPQPLSVPPVRDMDEALETFGPGPDGVEPLLKPALPELAPIHFHPSR
                     PHA"
     gene            1112..1798
                     /gene="narI"
                     /locus_tag="PKN55_14190"
     CDS             1112..1798
                     /gene="narI"
                     /locus_tag="PKN55_14190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017507101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HNX72443.1"
                     /translation="MDTLHQFLFGIYPYIALSVFLLGSLIRFEREQYSWKSESSQLLH
                     RGSLRLGSMLFHIGVLGLFFGHAVGLLTPVAVWDALGVSHSFKQVFAMTAGGVMGTLC
                     LLGLLMLLSRRLGNARLAANTTWRDTLVLGWLLVTVLAGLSTIFVSAQHLDGHEMVLL
                     MSWAQHIVTLRGDAASFITDASPVFKLHLFLGMSLFVIFPFTRLVHVWSGFAAVGYLG
                     RAWQLVRPRR"
BASE COUNT          303 a          611 c          558 g          339 t
ORIGIN      
        1 tcggtgaaag acctgcgcat cccgattcaa tacctggcca atctgctgac cgccggcaag
       61 gaagagccgg tgatcaaagc gctggaaacc atggtcgcca tgcgccgcta tatgcgcaaa
      121 aagagcgtgg acaaggtggt ggaccccaac acgctcaagg gcacccacct gaacccggcg
      181 caagtggaag acatgtacca ggtgatggcc atcgccaatt acgaagaccg ttttgtcatc
      241 ccgtccagcc acaaggaaat ggtggaaaac gccttcgaag acaaagccag ctgcggcttt
      301 acctttggca acggctgctc ggatggccac tccaatgaaa gcctgtttgg ccgcaaaaaa
      361 gccacgccga ttgtctttca cgacatgcgc cgcgaccgcg cgcgccaggc cggggaataa
      421 tgtcatgtct acacccaccc atcctttgag ttatcgcgtg ctgtcgctgc tgctggacta
      481 cccggaacag ccgctgatcg acgcgctgcc cgagctggac agcgcgctgg ccgatgttga
      541 accggcgctg tgcgcccagc tggcgccgct gttcacccac ctgggcggcg agccctcgct
      601 gattgccttg caggaaacct acgtcggcac gtttgaccgc cagccggcgc actcgctgca
      661 cctgtttgag cacctgcacg gcgaagaccg cgcccgtggc caggcgatgg tggacctgat
      721 cgaggaatac cgcgcccacg gctttgagcc agtcagccac gagctgccgg attatctgcc
      781 gctgtttctg gagtttctgt cgctgtgcgc cgccgacgag gcgcgccggc tgctggacga
      841 cgccgtgcat gtggtggcgc acatcggccg ccagctggcc tcgcgcggct cgccctacgc
      901 ctgcgtgctg caagccctgg ccgcgctggc cagcgtggag ccgcaaccat tgagcgtgcc
      961 gcccgtgcgc gatatggacg aagcgctgga aaccttcggc cctggcccgg acggcgtcga
     1021 gcccctgctc aaaccggcgc tgcccgagct ggcgccgatc cattttcacc ccagccgccc
     1081 gcacgcctga cggccggcaa ggagagaccc catggatacc ctgcatcaat ttctgttcgg
     1141 gatttacccg tacatcgcgc tcagcgtctt tttgctgggc agcctgatcc gcttcgagcg
     1201 cgagcaatac agctggaaaa gcgaatccag ccagctcttg catcgcggca gcctgcgcct
     1261 gggcagcatg ttgtttcaca tcggcgtgct gggcctgttt tttggccacg ccgtcggcct
     1321 gctcaccccg gtggcggtat gggacgcgct gggcgtgtcg cacagcttca agcaagtgtt
     1381 cgccatgacc gctggcggcg tgatgggcac gctgtgcctg ctgggcctgt tgatgctgct
     1441 gtcgcgccgc ctgggcaacg cccgcctggc cgccaacacc acctggcgcg acaccctggt
     1501 gctgggctgg ctgctggtga ccgtgctggc tggtttgtcc accatctttg tctccgccca
     1561 acacctggac ggccacgaaa tggtgctgct gatgagctgg gcgcagcata tcgtcaccct
     1621 gcgcggcgac gccgccagct tcatcaccga cgcctcgcca gtgttcaagc tgcacctgtt
     1681 tctgggcatg agcctgtttg tgatcttccc gttcacccgc ctggtgcacg tgtggagcgg
     1741 ctttgccgcc gtgggctacc tgggccgcgc ctggcaactg gtgcgcccac gccgctgagc
     1801 gcggtggccc a
//
LOCUS       DAOPYX010000431         1797 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_99642_length_1797_cov_10.792193, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000431 DAOPYX010000000
VERSION     DAOPYX010000431.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 1797)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1797)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1797
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_99642_length_1797_cov_10.792193"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..525
                     /locus_tag="PKN55_14195"
     CDS             <1..525
                     /locus_tag="PKN55_14195"
                     /inference="COORDINATES: protein motif:HMM:NF019005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030701 - NAD+-dinitrogen-reductase
                     ADP-D-ribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)--dinitrogen-reductase
                     ADP-D-ribosyltransferase"
                     /protein_id="HNX72444.1"
                     /translation="SVLRGWSINADSREGAVLKGWVESRFGLTPRFHGEPLRDPSAPA
                     YRHYLEMRAAGLYGTHALEAQLDLVYSYCQYELAHAWPADTLLTLYRGVNHMDDFEQL
                     GGDARRPRLLLNNLNSFTGQRERADEFGDYILTVRVPTPLIVCYCGLLPGALRGEDEY
                     LVLGGVYDVTRSTL"
     gene            641..1483
                     /locus_tag="PKN55_14200"
     CDS             641..1483
                     /locus_tag="PKN55_14200"
                     /inference="COORDINATES: protein motif:HMM:NF024685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HNX72445.1"
                     /translation="MTPALRATLIAGIQDGSIVPYLGCGVLADVRHTDTGAAIPADSD
                     SLILAMNNGRPMSPKLMYEFPRAAMNVELKRGRGAVTRFLDQVYGDSRWTRAAVHDWL
                     RAIRPPYVIDVNRDTQLQDSYADTPHTLIVGVARVTGDGTRYRLFAWDGEKYTPTSDL
                     APGQPILFKPMGTPRPGGAGYIASDADYVDYITELMGGFAIPPTLKTLRQGKRYLLMG
                     LRLNRDTERMVMADIIYAAHTSPGWVLIAEPTAKEERFCARLGLEIIRADIGELIGET
                     AMAA"
     gene            1596..>1797
                     /locus_tag="PKN55_14205"
     CDS             1596..>1797
                     /locus_tag="PKN55_14205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX72446.1"
                     /translation="MLMTRYEPLIFNANFTETSSDGKYVAYRGELTICEGEVADAQGR
                     RKPPTEVLKQVAVLGEGDSLKFL"
BASE COUNT          337 a          636 c          519 g          305 t
ORIGIN      
        1 agtgtgctgc gcggctggag catcaacgcc gacagccgcg aaggcgccgt gctgaaaggc
       61 tgggtggaat cacgctttgg cctgaccccg cgttttcatg gcgagccgct gcgcgacccc
      121 tcggcgccgg cctaccgcca ctatctagag atgcgcgccg ccggcttata tggcacccac
      181 gcgctggaag cccagcttga tctggtgtac agctattgtc agtacgagct ggcgcatgcc
      241 tggccggccg acacgctgct gacgctgtat cgcggcgtca atcacatgga cgactttgaa
      301 caactcggcg gcgacgcccg ccgcccgcgc ctgctgctga acaatctgaa ttcgtttact
      361 ggccagcgcg aacgcgccga tgaatttggc gactacatcc tgacagtgcg cgtgcccacc
      421 ccattgatcg tctgttattg cggcctgctg cccggcgcct tgcgcggcga agacgaatat
      481 ctggtgctgg gcggcgttta tgatgtgacg cgctccaccc tgtagcactt acgacaaaag
      541 cacacaaacc acacgctaac tgcttgtttt gtaatgcagc gcatgcaggt gtggaatttg
      601 ctgtgaggcg ggtgtccccc attcagcccg gatacccgcc atgacccctg ccctgcgcgc
      661 caccctgatc gccggcatcc aagacggcag catcgtcccc tacctcggtt gcggggtgct
      721 ggccgacgtg cgccacaccg acaccggcgc cgccatcccc gccgacagcg attcgctgat
      781 tctggccatg aacaacggcc ggccgatgtc gcccaagctg atgtatgaat ttccgcgcgc
      841 ggcgatgaat gtcgagctca agcgcggccg tggcgccgtc acccgctttc tggaccaagt
      901 gtatggcgac agccgctgga cccgcgccgc cgtgcacgac tggctgcgcg ccatccgccc
      961 gccgtatgtg attgacgtca accgcgacac ccagctgcaa gacagctacg ccgacacccc
     1021 gcacaccctg attgtgggcg tggcccgcgt caccggcgac ggcacccgct accggctgtt
     1081 tgcctgggat ggcgaaaaat acacccccac cagcgacctg gcccccggcc aacccatcct
     1141 gttcaagccg atgggcacgc cgcgccccgg cggcgccggc tatatcgcct cggacgccga
     1201 ctatgtggac tacatcaccg agctgatggg cggctttgcc atcccgccca ccctgaaaac
     1261 cctgcgccag ggcaagcgtt acctgctgat gggcctgcgc ttgaaccgcg acaccgaacg
     1321 catggtgatg gccgacatca tctacgccgc ccacacctcg ccgggctggg tgctgattgc
     1381 cgaacccacc gccaaggaag aacgcttctg tgcccgactg ggcctggaaa tcatccgcgc
     1441 cgacatcggc gagttgatcg gcgaaaccgc catggccgcc tgacaccaca ccctgatgcg
     1501 acgcccacgc cgacagccgg cccaggcaga ccgcacccgc gctgcgtcag gattgctttt
     1561 gactcaccct ccacccactt gcaaggaatg cgaccatgct gatgacccgc tacgaaccgt
     1621 tgattttcaa cgccaacttc accgaaacct ccagcgacgg caaatacgtt gcctatcgcg
     1681 gcgagctgac catctgcgaa ggcgaagtgg ccgacgccca gggccgccgc aagccgccga
     1741 ctgaagtgct caagcaagtg gccgtgctgg gcgaaggcga cagcctgaaa ttcctga
//
LOCUS       DAOPYX010000432         1797 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_99643_length_1797_cov_9.094145, whole genome shotgun sequence.
ACCESSION   DAOPYX010000432 DAOPYX010000000
VERSION     DAOPYX010000432.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 1797)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1797)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1797
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_99643_length_1797_cov_9.094145"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..215
                     /locus_tag="PKN55_14210"
     CDS             <1..215
                     /locus_tag="PKN55_14210"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HNX72447.1"
                     /translation="AQVALAWLLHKHPQLIAIPGARKLPHLRANAAAAELALSAAELA
                     ELDAMFDPAKVSGARYPDAGWAGIER"
     gene            581..1612
                     /locus_tag="PKN55_14215"
     CDS             581..1612
                     /locus_tag="PKN55_14215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX72448.1"
                     /translation="MTVPSQQLHAIHSMLTAGQRNLRLERHTLWLWGVPAGVLFVLSE
                     HILTPEQFPDLTQRALAWLALLLTVLATVATLDWHWTRQAKQTRDEAWSFIHRQVVKV
                     LWLLMGLATLTTFAMFFYGGGYMVCAVWLVFLGVSLYLHGLFSEELLEWAGLLTIALG
                     IVSLLARLPYDSMRWVAAAVFGLGLPMLSLMLDHGRHRPASLRLGQMLAWLSVVVLAP
                     LTLDRLLHQTPPIELPITPLREFHQSQPGAQVVRLPVGTVIPVQIELAGDVFASPSPV
                     QLPLTLRQPVDVLLKNGQLSGEARIPGEPWLRRDTRWLNIPWLKADLPPGGQPAVRTQ
                     LIVRVGGQP"
BASE COUNT          297 a          554 c          570 g          376 t
ORIGIN      
        1 acgcccaggt ggcgctggcg tggttgctgc acaagcaccc gcagctgatc gccatccccg
       61 gtgcgcgcaa attgccgcac ctacgcgcca acgccgccgc cgcagagctg gcgctgtcgg
      121 cagcggagct tgccgagctg gatgccatgt tcgacccggc caaggtaagc ggcgcgcgtt
      181 acccagatgc cggctgggcg gggattgaac gctaagtggc ttctggcggt gtagcgtggc
      241 ccgactgttg ccagcaccac accaaaaggg aagcgccacg cgatttcaga ttgactgggc
      301 atcttgttgc ttgacactct gttttacaga gtaataaact ctgcaaaaca gagtaaacgc
      361 cattcacacc agacgctggc acaatgacgt tcacgctgat gacctagtag tcagtcacca
      421 tgatttgaac cgacaagccg gagcttagac cgcagtacgc tgtagatacg cctggattcc
      481 cgcctgcgcg ggaatgactc ggtgtagtgc gacgaggagt tcttcgtgac tcatgaaccc
      541 tcgcgtttca acgtgactgg ctactagccc aggactcgcc atgaccgtcc ccagccaaca
      601 attacacgcc attcacagca tgcttaccgc cggccagcgc aatctgcgtc tggagcgcca
      661 caccctatgg ttatggggag tgccggccgg tgtgctgttt gtgctgtctg agcacattct
      721 cacccccgag cagtttcccg acctcaccca gcgcgcgctg gcctggctgg cgctattgct
      781 gacggtgctg gccacggtgg ccacgctgga ctggcactgg acgcgacagg ccaagcaaac
      841 ccgtgacgag gcttggtcgt ttattcaccg tcaggtggtc aaggtgttat ggctgttgat
      901 gggcctggcc acgctgacca cctttgccat gtttttttat ggcggcggtt atatggtgtg
      961 cgcggtgtgg ctggtgtttc tcggcgtcag cctgtatctg cacgggctgt tttctgaaga
     1021 gctgctggaa tgggcggggc tgctgaccat tgcgctgggc attgtcagcc tgctggcgcg
     1081 gctgccctac gacagcatgc gctgggtggc ggcggcggtg tttggcctgg gcctgccgat
     1141 gttgtcgctg atgctcgacc acggccgcca tcgcccggcc tcgttgcggc tggggcagat
     1201 gctggcctgg ttgtcggtgg tggtactggc cccgctgacg ctggatcgcc tgctgcatca
     1261 aacgccgccg atcgagctgc cgattacccc cttgcgcgaa ttccaccagt cgcagccagg
     1321 cgcgcaagtg gtgcgtctgc cggtgggcac ggtgattccg gtgcaaatcg agctggccgg
     1381 cgatgtgttc gccagcccaa gcccggtgca gttgccgctg accttgcgcc agccggtgga
     1441 tgtgctgctg aaaaacggcc agctcagcgg cgaggcgcgc attcctggcg agccgtggct
     1501 gcgccgcgac acccgctggc tgaatattcc ctggctcaag gcggatctac cgcccggcgg
     1561 ccagccggcg gtgcgcaccc agctgattgt gcgcgtgggc ggccaaccct gacacggcag
     1621 cgccctttct aagagccgct aacaaaaccc cctggcgttg ttgtgtgacc ttgccgtact
     1681 gcttgtactg tctgcggtca cacgcctagc cagggccgct tcgctggatt ttgtgagcgg
     1741 ctcttaaaac atccctgtgt tgtgtgtata tccagccgca tggcgcgccg gagggct
//
LOCUS       DAOPYX010000433         1775 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_101599_length_1775_cov_5.061047, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000433 DAOPYX010000000
VERSION     DAOPYX010000433.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 1775)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1775)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1775
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101599_length_1775_cov_5.061047"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            163..1038
                     /locus_tag="PKN55_14220"
     CDS             163..1038
                     /locus_tag="PKN55_14220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008952912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HNX72449.1"
                     /translation="MLLRIASILLPVLSIVLIGYLYGRRHAPDMVTVNRINMDLFVPA
                     LVFVALAGKDVQLAQYGWLALAAAGVVLGSGLLAWPLARLLGIAPLTFVPPMMFNNSG
                     NMGLPLIVLAFGAAALPGAVILFLVEMILHFSLGLWLMDRHAPIGRLFRQPVVIATLA
                     GLAVSVSGLVLPDAVALALKMLGDVSVPLLLFSLGVRIAGVSWAEWKLGVLGGLICPA
                     SGLAMAAAMVAWLPLPAAQQPWLWLFAALPPAVLNFLVAEQYRQEPSRVASLVLIGNL
                     MSVLTLPPVLYWLLS"
     gene            complement(1162..1647)
                     /locus_tag="PKN55_14225"
     CDS             complement(1162..1647)
                     /locus_tag="PKN55_14225"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HNX72450.1"
                     /translation="MNDAPLYAFTLDRLDGGALPLSDYRGQVLLLVNTASQCGFTGQY
                     AGLEALYRQYGPRGLVVIGCPCNQFGRQEPGDAADIGAFCQRNYGVSFPLSAKLEVNG
                     DGTHPLWQHLKAAQPGVLGSQAIKWNFTKFLVNRQGEVVARYAPQTTPDALAADIEAL
                     L"
BASE COUNT          242 a          501 c          623 g          409 t
ORIGIN      
        1 tgaagaaatg gtgcatgact tgcgggtgct gctgcgtctg gcggatggcc gcaagaccca
       61 cccatccgcc gccatcatca gagagaattt ctcccggttt ttgattgact tcggccgttt
      121 ggtctgacca tccgaccttg accccaccag gagcgtgcgc cgatgttatt gcggattgcc
      181 tcgattttat tgccggtgct gtccattgtg ctgatcggct atctgtatgg ccgtcggcat
      241 gcgccggata tggtgacggt gaaccgcatc aatatggatt tgtttgtgcc ggcgctggtg
      301 tttgttgcgc tggccggcaa ggatgtgcag ttggcgcaat atggctggct ggcgttggcg
      361 gcggccgggg tggtgttggg ctcggggctg ttggcctggc ccttggcgcg gctattgggc
      421 attgccccgc tgacttttgt gccgccgatg atgttcaata actccggcaa tatgggcctg
      481 cccttgattg tgctggcgtt tggcgcggcg gcgctgccgg gtgcggtgat tttgtttctg
      541 gtggagatga ttctgcattt ctcgctgggg ctgtggctga tggatcgcca cgcgccgatt
      601 ggccggctgt ttcgccagcc ggtggtgatc gccaccttgg ccgggctggc ggtgagtgta
      661 tccggcttgg tcttgccgga cgcggtggcg ctggccttga agatgctggg cgatgtgtcg
      721 gtgcccttgc tgctgttttc gctgggggtg cgcattgccg gggtgtcgtg ggcggagtgg
      781 aagctgggcg tgctgggcgg tttgatctgc ccggccagcg ggctggcgat ggcggcggcg
      841 atggtggcct ggctgccgct gccggcggcg caacagccct ggctgtggct gtttgccgcg
      901 ctgccgccgg cggtgctgaa ttttttggtg gccgagcaat accggcagga gcccagccgg
      961 gtggcttcgc tggtgctgat tggcaatttg atgagcgtgc tgacgctgcc gccggtgttg
     1021 tattggctgt tgagttagcg ccgctcaccc caccctgctt gcccgacctt gccgaatggt
     1081 ctgcgctggt gcgcctgtgc tgagtttttc gcggcgcgtg ggcagttcgt gacttgcgct
     1141 cacggctgcg tcgcgcggcg cttacaacag cgcctcgata tccgccgcca gcgcatccgg
     1201 cgtggtctgc ggcgcgtagc gcgccaccac ttcgccctga cggttgacca gaaacttggt
     1261 gaagttccat ttgattgcct ggctgcccag cacgccgggc tgggcggctt tcaggtgttg
     1321 ccacagcggg tgggtgccat cgccgttgac ctccagcttg gccgacagcg gaaaactcac
     1381 gccgtagttg cgttggcaaa acgcgccgat gtcggcggcg tcgcccggct cctggcggcc
     1441 aaactggttg cacgggcagc caatcaccac caagccgcgc gggccatact ggcgatacag
     1501 cgcttccagc ccggcgtact ggccggtaaa gccgcactgg ctggcggtgt tgaccagcag
     1561 cagcacctgg ccgcgataat cgctcagcgg cagcgcgccg ccgtccaggc ggtccagggt
     1621 gaaggcgtac aagggggcgt cgttcatggg gggctttcgg gggcaaaaat cggtgaatgt
     1681 gttcactatg gcaaagccaa tctgcttcgg caacggcgat tgaatcttgc tgcgcagcag
     1741 cagccttgtt actatcaaaa gacacacaag cttca
//
LOCUS       DAOPYX010000434         1766 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_102402_length_1766_cov_13.516072, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000434 DAOPYX010000000
VERSION     DAOPYX010000434.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 1766)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1766)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1766
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102402_length_1766_cov_13.516072"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..870)
                     /locus_tag="PKN55_14230"
     CDS             complement(<1..870)
                     /locus_tag="PKN55_14230"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNX72451.1"
                     /translation="MSSLSLPSPTLSTPAWRIAVVGCAFSLIWSSAFIAGKQAMTGCG
                     PFTLLSLRFLAAGALLWLLRRWLPGDTPQQAAAPAIAWRHALIAGLLTNVVYLGLGYH
                     GLRHVPAGLTAILVSVSPLLTAAMAALWLREPLGARQLLGLLCGMGGVAWIMAGRSGG
                     ASALDHKAWLGVGMILAGALALAASTLYNRRAAGQLNPWRIARLQLWASGLTLLPLAL
                     WTEGLQLNPTPLVLASLAYQATVVSIGTTLMLLWLVRHGGAAKASSFHLLNPLFGVLL
                     AAALLGETVTLSDV"
     gene            987..>1766
                     /locus_tag="PKN55_14235"
     CDS             987..>1766
                     /locus_tag="PKN55_14235"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HNX72452.1"
                     /translation="MSWHTRHLELDWLRALVTIVDCGGFSAAAEQLGRSQSAISLQIK
                     RLEDTVGQPVLRRSQGRVDGPTSEGRVLLDYARRMLRLNDEAWACFAQPQMAGRLRLG
                     LPEELMEQVFAPVQAAFARLYPRVSLSVRCDLSVRLAALAEAGELDLALAKRVGGQPA
                     SLSGEAWRVLRREPLHWFDAEGSNARDGRPLPLAVFHEGCVFRGAALSALHDAGLSGE
                     VRFVGSSIGAMRHAVLAGLALAPLPASLAAPGMRSANDSLPP"
BASE COUNT          275 a          586 c          651 g          254 t
ORIGIN      
        1 tacatcgctc agcgtcaccg tttcgcccag cagcgccgcc gccagcagca cgccaaacag
       61 cggattgagc aggtgaaagc tgctggcctt ggcggcgccg ccgtggcgca ccagccacag
      121 cagcatcagc gtggtgccaa tcgacaccac ggtggcttga taggccaggc tggccagcac
      181 cagcggggtg gggttgagct gcaggccttc cgtccacagc gccagcggca gcagggtcag
      241 gccgctggcc cagagttgca ggcgggcgat gcgccagggg ttgagctggc cggcggcgcg
      301 gcggttgtac agggtggacg ccgccagcgc cagcgcgccg gccaggatca tgcccacgcc
      361 cagccaggcc ttgtggtcca gcgcgctggc gccgccgctg cggccggcca tgatccacgc
      421 cacgccgccc atgccgcaca gcaagcccaa cagttggcgc gcgcccagcg gctcgcgcag
      481 ccacagcgcc gccatggcgg cggtcagcag cgggctgacg ctgaccagaa tcgccgtcag
      541 cccggccggc acatggcgca ggccgtgata gcccagcccc agatacacca cattggtcag
      601 caagccggcg atcagcgcgt gccgccaggc gatggccggc gcggcggctt gttgcggcgt
      661 gtcgccaggc agccagcggc gcagcagcca cagcagcgcg ccggccgcca aaaagcgcag
      721 gctgagcagg gtgaacgggc cgcagccggt catcgcctgc ttgccggcaa taaaggccga
      781 gctccagatc aggctgaagg cgcagccgac cacggcaatg cgccaggcgg gggtggacag
      841 cgtaggggac ggcagggaca ggctggacat cacggtctcc gtgggggtca ggacatggcc
      901 acactgtatc ggtgcgccga gtatttgcaa aattgataat cctgccttga taatcagctt
      961 tatcaatacc catcctggag ccgcgcatga gctggcacac ccgacacctg gagctggatt
     1021 ggctgcgcgc cttggtgacc attgtcgatt gcggcgggtt ttctgccgcc gccgagcagc
     1081 tgggccgcag ccaatcggcc atcagcctgc aaatcaagcg gctggaagac accgtcggcc
     1141 agccggtgtt gcggcgcagt cagggccggg tggacggccc gaccagcgag ggccgggtgc
     1201 tgctggacta cgcccggcgc atgttgcggc tgaacgacga ggcctgggcc tgctttgccc
     1261 agccgcagat ggccggccgc ttgcggctgg gcctgccgga agaattgatg gaacaggtgt
     1321 tcgccccggt gcaggcggcg tttgcccggc tgtatccgcg cgtgagcttg tcggtgcgct
     1381 gtgatttgtc ggtgcggctg gcggcgctgg ccgaggccgg cgagctggat ttggcgctgg
     1441 ccaagcgggt gggcgggcaa ccggccagcc taagcggcga agcctggcgg gtgttgcgcc
     1501 gtgagcccct gcattggttt gacgctgaag gcagcaatgc ccgcgacggc cggccgctgc
     1561 cgctggcggt gtttcatgag ggctgcgtgt ttcgcggcgc ggccttgagc gcgctgcacg
     1621 acgccggctt gagcggcgag gtgcgttttg tcggcagcag catcggcgcc atgcgccacg
     1681 cggtgctggc cgggctggcg ctggcgccgc tgccggccag cctggcggcg ccgggcatgc
     1741 gcagcgccaa cgacagcctg ccgccc
//
LOCUS       DAOPYX010000435         1757 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_103245_length_1757_cov_13.478261, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000435 DAOPYX010000000
VERSION     DAOPYX010000435.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 1757)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1757)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1757
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_103245_length_1757_cov_13.478261"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            107..1015
                     /locus_tag="PKN55_14240"
     CDS             107..1015
                     /locus_tag="PKN55_14240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018152119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNX72453.1"
                     /translation="MTIALDIQAVSKRFGALTALDQVSLSVEAGEFFALLGPNGAGKT
                     TLISCMAGLSRPDSGHIRVMGHDVQHDYRAARRALGVVPQELVFDPFFTVRESLVFQS
                     GYFGLKHNDNWIDEILHKLGLTSKANANMRALSGGMKRRVMVAQALVHRPPVIVLDEP
                     TAGVDVELRQSLWVFIQELNRAGHTIVLTTHYLEEAETLCNRIAMLKAGRVIALEDKA
                     TLLGQGSARRDIMLRLDPPGLPPALEPLLLRREGSKVWLRLADIDQLGGVLAQVQAAG
                     ARVRDFEVVELDLEQVFVARMQGKEA"
     gene            1015..>1757
                     /locus_tag="PKN55_14245"
     CDS             1015..>1757
                     /locus_tag="PKN55_14245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNX72454.1"
                     /translation="MSGFYTLFYKEILRFWRVAFQTVAAPVLTALLYLMIFSHALSRH
                     VDVFPGVGYTAFLIPGLVMMSMLQNAFANTSSSLIQSKITGNIVFLLLPPLSHLEFFT
                     AYVLAAVVRGLVVGVGVFAVTLWFDLPALSHPLWALAFAVLGCAVLATLGLIAGIWAD
                     KFDQLAAFQNFLIMPLTFLSGVFYSIHTLPPFWRGLSHANPVFYMIDGFRYGFFGQSD
                     APPLLSLAVVSGGLLVLMALALRLLRAGY"
BASE COUNT          259 a          494 c          597 g          407 t
ORIGIN      
        1 gtctgaccgc tcaattaggg caatactgtc atcagcgcgc cgcgtgatgc cgctgacgca
       61 ccttgctgat tttccggtaa actcacccct ttttgccgcc gcgccaatga ccattgcgct
      121 cgatattcag gcggtcagca agcgctttgg cgcgctgact gcgctagacc aggtttcgct
      181 gtctgtagag gctggcgaat tctttgccct gctggggcca aatggcgccg gcaagaccac
      241 gctgatttcc tgcatggccg gtttgtcgcg gccggacagc ggccacatcc gggtgatggg
      301 ccacgatgtg cagcacgact accgcgccgc ccggcgcgcg ctgggcgtgg tgccgcagga
      361 gttggtgttt gatccgtttt ttaccgtgcg cgagtcgctg gtgtttcagt cgggctattt
      421 tggcctgaag cacaacgaca actggattga cgaaatcctg cacaagctgg ggctcaccag
      481 caaggccaac gccaatatgc gcgcgctgtc gggcggcatg aagcggcggg tgatggtggc
      541 gcaggcgctg gtgcaccgtc cgccggtgat tgtgctggac gagcccaccg ccggggtgga
      601 tgtcgagctg cggcaaagcc tgtgggtgtt tattcaagag ctcaaccgcg ccggccacac
      661 cattgtgctg accacccatt atctggaaga agcggaaacc ctgtgcaacc gcatcgccat
      721 gctcaaggcc gggcgggtga ttgcgctgga agacaaggcc acgctattgg gccagggcag
      781 cgcgcgccgc gacatcatgc tgcggctgga tccgcccggc ttgccgccag cgctggagcc
      841 cttgttgctg cgccgcgagg gcagcaaggt gtggctgcgg ctggccgaca tcgaccaatt
      901 gggcggggtg ttggcccagg tgcaggcggc gggggcgcgg gtgcgcgatt ttgaagtggt
      961 cgagctggat ctggagcagg tgttcgtggc gcgcatgcaa ggcaaggagg catgatgagc
     1021 gggttttaca cgctgttcta caaagaaatc ctgcgctttt ggcgggtggc cttccagacc
     1081 gtggcggcgc cggtgctgac ggcgctgttg tatttgatga ttttttcgca tgcgctgtcg
     1141 cgccatgtgg atgtgtttcc cggcgtgggc tatacggcgt ttttgattcc gggcctggtg
     1201 atgatgtcga tgctgcaaaa cgccttcgcc aatacgtctt ccagcctgat tcagtcaaaa
     1261 atcaccggca atatcgtgtt tttgctgctg ccgcccttgt cgcacctgga gtttttcacc
     1321 gcctatgtgc tggcggcggt ggtgcgcggg ctggtggtgg gtgtgggggt gtttgcggtg
     1381 acgctgtggt ttgacctgcc agcgctgagc cacccgctgt gggcgctggc ctttgccgtg
     1441 ctgggctgcg cggtgctggc gacgctgggc ttgattgccg gcatttgggc ggataaattc
     1501 gaccaactgg cggcgtttca gaactttttg atcatgccgc tgactttttt gtcgggcgtg
     1561 ttttattcga ttcacaccct gccgccgttc tggcgcgggc tgtcgcacgc caacccggtg
     1621 ttttatatga tcgacggctt tcgctatggc ttttttggcc agtccgacgc cccgccgctg
     1681 ctgagcctgg cggtggtcag cggcggcctg ttggttttga tggcgctggc gctgcgcctg
     1741 ctgcgcgccg gctacaa
//
LOCUS       DAOPYX010000436         1757 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_103321_length_1757_cov_2.213278, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000436 DAOPYX010000000
VERSION     DAOPYX010000436.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 1757)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1757)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1757
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_103321_length_1757_cov_2.213278"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..400
                     /locus_tag="PKN55_14250"
     CDS             <1..400
                     /locus_tag="PKN55_14250"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNX72455.1"
                     /translation="GWKMDEFAQQLRQHPQAGKNLLWLEGASDEYLEKIYARADCLIA
                     ASECEGFGLPLIEAAQHKLPVLARDIAVFREVAGEHAAYFCARQPQELAQALRDWLRL
                     FAADAHPRSDSMPWLSWRDSARALLRPIID"
     gene            414..1217
                     /locus_tag="PKN55_14255"
     CDS             414..1217
                     /locus_tag="PKN55_14255"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HNX72456.1"
                     /translation="MTEPTTPLAAAAPSPAQDAGAGAQLNQAVHQVITVHLNNIPLLS
                     GLSPQVLQQVGAAMQFRTVEKGTYVMLKGSPGEHLVFVLSGRVQVVDVTEDGREMVLA
                     TLAPGDYVGEMAVIDNQPRSASVLAADTSLVAFLPSAQARQLLYGQPLVAERVMRRLA
                     ATVRSAAANRAILSLPSAFQRVYAALEQLSQPAPGNMREIPAMPTQQEIAAMVNTSRE
                     TVSRALNVLLQKQIVEKDLRRLIVRQPDTLRKMAQEDPAPGPAGHAGGR"
     gene            1223..1510
                     /locus_tag="PKN55_14260"
     CDS             1223..1510
                     /locus_tag="PKN55_14260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX72457.1"
                     /translation="MPAGWRAWLLHLRNKLHEEDKQQHIEWSFWLTHAACLLWPLPWA
                     LAAVTATGLCKEIWDARYGSGFCWYDMLGNAIGIALALMMICLAPEGLYYP"
     gene            1507..>1757
                     /locus_tag="PKN55_14265"
     CDS             1507..>1757
                     /locus_tag="PKN55_14265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX72458.1"
                     /translation="MKLLVNGVPLLAPLTGIGQYTRQLFTALRADGLDPLMFYGLHCE
                     HGLPATAEVSGTARNTRRAYDLVRKVLPNPRELKRLVERA"
BASE COUNT          298 a          583 c          577 g          299 t
ORIGIN      
        1 aggctggaag atggacgaat tcgcccagca gttgcgccag catccacaag ccggcaaaaa
       61 cctgctgtgg ctggaaggcg ccagtgacga gtatcttgaa aaaatctatg ctcgggccga
      121 ttgtctgatt gccgcctcgg aatgcgaagg ctttggtctg ccgctgatcg aagccgccca
      181 gcacaagctg ccggtgctgg cgcgcgatat tgccgtgttc cgtgaagtgg ccggcgagca
      241 cgccgcgtat ttttgcgcca ggcagccgca ggagctggcc caggcgctgc gcgactggct
      301 gcgcctgttt gctgccgatg cacacccgcg ttcggacagc atgccgtggc tgagctggcg
      361 cgacagcgcc cgcgccctgc tgcgtccgat cattgactga ccctgagccc ctgatgactg
      421 aacccaccac cccactcgcc gccgctgccc cgtcgcctgc ccaggacgcc ggggccggcg
      481 cgcagctgaa tcaggcggtg catcaggtga tcaccgtgca cctgaacaac atcccgctgc
      541 tcagtggcct gagcccgcag gtgctgcaac aggttggcgc ggccatgcag tttcgcaccg
      601 tggaaaaagg cacgtatgtg atgctcaagg gcagccccgg cgagcatctg gtgtttgtgc
      661 tgtctggtcg ggtgcaggtg gtggacgtca ccgaagatgg ccgcgaaatg gtgctggcca
      721 ccctggcgcc gggcgattat gtgggtgaaa tggcggtgat cgacaaccag ccgcgttcgg
      781 cctcggtgct ggcggcggac acctcgctgg tggcgttttt gcccagcgcg caggcgcgcc
      841 agctcttgta tggccagccc ttggtggccg agcgggtgat gcgccggctg gccgccaccg
      901 tgcgctcggc cgccgccaac cgcgccattc tcagcctgcc cagcgccttt cagcgcgtat
      961 acgccgcgct ggagcagctg tcgcagccgg cgccgggcaa tatgcgcgaa atcccggcca
     1021 tgcccaccca gcaggaaatc gccgccatgg tgaacaccag ccgggagacg gtgtcgcgcg
     1081 cactgaatgt gctgctgcaa aagcagattg tcgaaaaaga cctgcgccgc ttgatcgtgc
     1141 gccagccaga caccctgcgc aaaatggcgc aggaagaccc cgcacccggc ccggccggcc
     1201 atgcgggcgg gcggtgagcc ggatgccggc cggctggcgc gcctggctgc tgcaccttcg
     1261 caataaactg cacgaggaag acaaacagca gcatatcgaa tggagtttct ggctcaccca
     1321 cgccgcctgc ctgctgtggc cgctgccctg ggcgctggcg gcggtgacgg ccaccggcct
     1381 gtgcaaggaa atctgggacg cccgctacgg cagcggcttt tgctggtatg acatgctggg
     1441 caacgccatt ggcatcgccc tggcgctgat gatgatctgt ctggccccgg aagggcttta
     1501 ttacccatga aactgcttgt aaacggcgtg cccttgctcg ccccgctgac cggcatcggc
     1561 cagtacaccc ggcaactgtt caccgccctg cgcgcggacg ggcttgatcc gctgatgttt
     1621 tacggcctgc actgcgaaca cggcctgcca gcaacggcgg aggtctcggg cacggcgcgc
     1681 aatacccgtc gcgcctacga tctggtgcgc aaggtgctgc ccaatccgcg cgagctcaag
     1741 cgcctggtcg agcgcgc
//
LOCUS       DAOPYX010000437         1753 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_103606_length_1753_cov_8.213192, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000437 DAOPYX010000000
VERSION     DAOPYX010000437.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 1753)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1753)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1753
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_103606_length_1753_cov_8.213192"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            209..1681
                     /gene="trpE"
                     /locus_tag="PKN55_14270"
     CDS             209..1681
                     /gene="trpE"
                     /locus_tag="PKN55_14270"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018749040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HNX72459.1"
                     /translation="MLTQAEFDALARQGFNRIPVILSLFADLDTPLSVYLKLANQPYS
                     YLLESVVGGERFGRYSFIGLPAATRLRVNGLRVQVEHGDRVVETHEGDPLGFIEAFQA
                     RFTTPPHDGLPRFAGGLVGYFGYETIKLIEKKLAARPAKPDPIGTPDILLMQSEELAV
                     VDNLSGQLYLIVYADPAVPNAYQKAQTRLAQLRAQLRQPSPLPLQGPAPRSEASSEFG
                     EDAFKQAVLRAKEYIIDGDIMQVVLSQRMRMPFAEPPLSLYRALRSLNPSPYMYFYHF
                     DDFHVVGSSPEILVRQENHTVTVRPIAGTRPRGQNRIEDEALAEDLLADPKEIAEHVM
                     LMDLGRNDVGRVADIGSVKITENMIIERYSHVMHIVSSVEGRLREGMSNLDVLRATFP
                     AGTLSGAAKVRAMEIIDELEPSKRGIYGGAVGYLGFNGDMDLAIAIRTAVVKDQMLYV
                     QAGAGIVADSQTEAEWQETRNKARAVIRAAELVQQGLDAE"
BASE COUNT          337 a          567 c          518 g          331 t
ORIGIN      
        1 cgcgccgcac aggctggcga acagaacagc gcctgcatcc atgcccttgt gcctggcggc
       61 aaaatgggca agaatcctgc cattgccttt tatccactca gaatccgatt gcctgctgtc
      121 atgaccgcgt atccgaacac tttgcgatgg cgtcgctagc cagacgttca ccccgcctga
      181 tttcattgat tactgttcgg agcctgtcat gctgactcaa gccgagttcg acgcgctggc
      241 gcgtcaaggt tttaaccgca ttcccgtcat tctctcgctg tttgccgacc tggacacccc
      301 gctgtcggtc tacctcaagc tggccaacca gccgtattcc tatctgctgg aatcggtggt
      361 cggcggcgag cgctttggcc gttattcttt cattggcctg ccggccgcca cccgcttgcg
      421 cgtcaacggc ctgcgcgtgc aggtggagca cggcgaccgc gtggtggaaa cccacgaagg
      481 cgacccgctg ggctttatcg aagccttcca ggcgcgcttc accacgccgc cgcacgacgg
      541 cctgccgcgc tttgccggcg gcctggtggg ctattttggc tacgaaacca tcaagctgat
      601 cgagaaaaaa ctcgccgccc gccccgccaa gcccgacccg attggcacgc cggatatctt
      661 gctgatgcaa tccgaagaac tggcggtggt ggataatctg tccggccagc tgtatctgat
      721 cgtctacgcc gacccggcgg tgcccaacgc ctaccaaaaa gcgcaaaccc gcctggcgca
      781 gctgcgcgcg caactgcgcc aaccctcgcc gctgcccttg caaggcccgg cgccgcgcag
      841 cgaggccagc tcggaatttg gcgaagacgc cttcaagcag gcggtgctgc gcgccaagga
      901 atacatcatc gacggcgaca tcatgcaagt ggtgctcagc cagcgcatgc gcatgccgtt
      961 tgccgagccg ccgctgtcgc tgtatcgcgc gctgcgcagc ctgaacccgt cgccgtatat
     1021 gtatttttat catttcgacg acttccacgt ggtcggctcc tcgccggaaa tcctggtgcg
     1081 ccaggaaaac cacaccgtca ccgtgcgccc gattgccggc acccgtccgc gcgggcaaaa
     1141 ccgcatcgag gacgaagcgc tggccgaaga cctgctggcc gacccgaaag aaatcgccga
     1201 acacgtcatg ctgatggacc tgggccgcaa cgatgtcggc cgggtggcgg atattggctc
     1261 ggtgaaaatc accgaaaaca tgatcatcga gcgctactcg catgtgatgc atattgtctc
     1321 cagcgtggaa ggccgcttgc gcgaaggcat gagcaatctg gacgtgctgc gcgccacctt
     1381 cccggccggc accctgtccg gcgcggccaa ggtgcgggcg atggaaatca tcgacgaact
     1441 ggaacccagc aaacgcggca tttatggcgg cgcggtgggc tatctgggct ttaatggcga
     1501 tatggacctg gccattgcca tccgcaccgc cgtggtgaaa gaccagatgc tctacgtgca
     1561 agccggcgcc gggattgtcg ccgactcgca aaccgaagcc gaatggcaag aaacccgcaa
     1621 caaggcgcgc gcggtgattc gcgcggctga gttggtgcag caggggttgg atgcggagta
     1681 atcaaggggc acgctagttg tcgcatcccg agtgatcata ttcccattcg tagctggcca
     1741 ctcttcttgg caa
//
LOCUS       DAOPYX010000438         1735 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_105300_length_1735_cov_10.319643, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000438 DAOPYX010000000
VERSION     DAOPYX010000438.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 1735)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1735)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1735
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_105300_length_1735_cov_10.319643"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(441..>1735)
                     /locus_tag="PKN55_14275"
     CDS             complement(441..>1735)
                     /locus_tag="PKN55_14275"
                     /EC_number="1.5.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017507262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004174 -
                     electron-transferring-flavoprotein dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="electron transfer flavoprotein-ubiquinone
                     oxidoreductase"
                     /protein_id="HNX72460.1"
                     /translation="NFCRWLGEQAEALGVEIYPGFAAAEVLYHEDGSVKGVATGDMGV
                     GRDGEPTDAYQPGMELWAKQTIFAEGCRGSLTKTLFERFDLRAECDVQTYGIGIKELW
                     EVKPEHHQPGSITHTTGWPMDSKTYGGSFLYHLENNQVVVGFVIGLDYENPHLSPYDE
                     FQRFKTHPAIRKVFEGGRRISYGARALSEGGIQSLPKLTFPGGALVGDTAGFLNVPKI
                     KGTHTAMKSGMLAAESIFAALGEDAPAREISSYTTAFRHSWLFDELWRVRNIRPSFHW
                     GFWPALVYSAIDTYLFKGNAPWTLHHKRADHECLKPAADMPKIQYPKPDGVLTFDKLS
                     SVFISNTNHSEDQPIHLRLKNPNTPIQVNLAQYDAPEQRYCPAGVYEIVREGDTAPRL
                     QINAQNCVHCKTCDIKDPTQNINWSTPEGGGGPNYPNM"
BASE COUNT          324 a          501 c          550 g          360 t
ORIGIN      
        1 tgccgtacag ggtcacgtcg gccatggcgg ccatcggcag ggcagcaact gccagagcga
       61 tcagtttttt catggggaaa atcctttcaa gtcagtcaaa gttcagttgg tcgcggcctg
      121 gccgcccaga aagaacgtct gtgcatcgcg caagagacgg cgacgtcatg gaggcgaata
      181 tagtcgttga cgttgtgaaa atacaaacac agcccgaata gttggcttcc gcagggcgga
      241 attacgctga cgtcaatcaa caagtctatg aataagatgg tgttttttgt aaattgccga
      301 ttgttgcatt cttgccacaa tatggcatgg atgtgacagt gcagcgcagc attgcacggc
      361 catccgtcgg cccgcacaga aaaaaaccgc gcgccggcag ggccgggcgc gcggtgtgac
      421 ggggcgatgc gcccggcact ttacatattg gggtagttcg gcccgccgcc gccttccggc
      481 gtgctccagt tgatgttctg ggtggggtct ttgatgtcgc aggtcttgca gtgcacgcag
      541 ttctgggcgt tgatctgcaa gcgcggcgcg gtgtcgccct cgcggacaat ctcgtacacc
      601 ccggccgggc aatagcgctg ttcgggcgcg tcgtactggg ccagattgac ctgaatcggc
      661 gtattcgggt ttttcagccg caggtgaatc ggctgatcct cgctgtggtt ggtgttggaa
      721 ataaacacac tggacagctt gtcgaaggtc agcacgccgt cgggtttcgg gtactgaatc
      781 ttgggcatgt ccgccgccgg cttcaggcac tcgtggtcgg cgcgcttgtg gtgcagcgtc
      841 cacggcgcat tgcccttgaa cagataggtg tcgatggccg agtaaaccag cgccggccag
      901 aagccccagt ggaaagacgg gcggatgttg cgcacgcgcc acagttcgtc aaacagccag
      961 ctatggcgaa acgccgtggt gtagctgctg atttcgcgcg ccggggcgtc ttcgcccagc
     1021 gcggcaaaaa tcgactcggc ggccagcatg ccggatttca ttgccgtgtg cgtgcccttg
     1081 atcttgggca cattcaaaaa gccggcggtg tcgcccacca gcgcgccgcc cggaaaggtc
     1141 agcttgggca ggctctgaat gccgccttca gacaaggcgc gcgcgccgta gctgatgcgc
     1201 cggccgccct cgaacacctt gcggatggcc gggtgggttt tgaagcgctg gaattcgtcg
     1261 taggggctca gatgcgggtt ttcgtagtcc aggccaatca caaagccgac caccacctgg
     1321 ttgttttcca ggtgatacag gaaagagccg ccataggtct tgctgtccat cggccagccg
     1381 gtggtgtggg tgatgctgcc cggctggtga tgctcgggtt tgacttccca cagttctttg
     1441 atgccgatgc cgtaggtttg cacatcgcac tcggcgcgca ggtcaaaacg ctcgaacagg
     1501 gttttggtca gcgagccgcg acagccttcg gcaaaaatgg tctgcttggc ccacagctcc
     1561 atgcccggct gatacgcatc ggtgggttcg ccatcgcggc ccacgcccat atcgccggtg
     1621 gccacgccct tgaccgaacc atcttcgtgg tacagcactt cggcggcggc aaagcccgga
     1681 tagatttcca cgcccagcgc ctcggcctgc tcgcccagcc agcggcagaa attgc
//
LOCUS       DAOPYX010000439         1706 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_108181_length_1706_cov_2.124167, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000439 DAOPYX010000000
VERSION     DAOPYX010000439.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 1706)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1706)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1706
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_108181_length_1706_cov_2.124167"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1642)
                     /locus_tag="PKN55_14280"
     CDS             complement(<1..1642)
                     /locus_tag="PKN55_14280"
                     /inference="COORDINATES: protein motif:HMM:NF037510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HNX72461.1"
                     /translation="MPALATRSLRDCQVSAVTGLEKSLALNKPRALVHMATGAGKTFT
                     AITSVYRLLKFGGAKRILFLVDTRNLGKQAHQEFMAYTPPDDGRKFTELYNVQRLNSP
                     TIDPHSQVCISTIQRMYSILSGEPIDESAEDVSLNEVQQTAKQEKLVRYNPAVPVETF
                     DFIIIDECHRSIYNLWKQVLDYFDASLIGLTATPDKRTFGFFNENIVAEYTYEQSVAD
                     GVNVGYNVYEITTEITQKGAVLKAKEWVDHRDRATRKKRWSETEEDTVYTGKELDKSV
                     VNVSQIRQVIQAMKTAVETQIFPARKETPKTLIFAKTDSHADDIIHIVREVYGQGNAF
                     CKKVTYRAEEDADSILASFRNDYHPRIAVTVDMIATGTDVKPLEVLLFMRDVRSKGYY
                     EQMKGRGVRSLDGDSLKRVSNSADGAKTHFVLIDAVGVEKSLKTDCRPLEKKPGVALK
                     DLLQGVAMGSRDDDTVLSLANRLLRLAKQLDDKARARIEKASGGVPVNELGKNLIAAL
                     DPDAIVQTALATARAQGITRNEDSLRPEEIDAARAKRVA"
BASE COUNT          267 a          455 c          546 g          438 t
ORIGIN      
        1 cggccacgcg cttggctcgg gcggcgtcga tttcttcggg tcgcaggctg tcttcgttgc
       61 gggtgatgcc ttgcgccctg gcggtggcca gggcggtttg cacgatggcg tcggggtcga
      121 gcgcggcaat caggtttttg cccagttcgt tcaccggaac gccgccgctg gctttttcga
      181 tgcgcgcgcg ggctttgtcg tccagttgtt tggccaggcg cagcaggcgg ttggccagcg
      241 aaagcacggt gtcatcgtcg cggctgccca tggccacgcc ttgcagcagg tctttaagcg
      301 ctacgccggg ttttttctcc agcgggcggc agtcggtttt caggcttttt tctacgccca
      361 cggcgtcgat cagcacaaag tgggtttttg cgccgtctgc gctattgctg acgcgcttga
      421 ggctgtcgcc gtccaggctg cgcacacccc ggcctttcat ttgttcgtag tagcctttgc
      481 tgcgcacgtc gcgcatgaac agcagcactt ccagcggttt gacatcggtg ccggtggcga
      541 tcatgtccac ggtgacggca atgcgtgggt ggtagtcgtt gcgaaagctg gccagaatgc
      601 tgtcggcgtc ttcttccgcg cggtaagtca ccttcttgca aaaggcattg ccctggccat
      661 atacctcgcg cacgatgtgg atgatgtcat cggcgtggct gtcggtcttg gcaaaaatca
      721 gggttttggg ggtttctttg cgcgcaggga atatttgcgt ttccacggcg gttttcatgg
      781 cctgaatcac ctggcgaatc tggctgacgt tgaccacgga tttatccagc tctttgccgg
      841 tgtaaacggt gtcttcttcg gtttcgctcc agcgtttttt gcgcgtggcg cggtcgcggt
      901 ggtccaccca ttctttggct ttcagcactg cgcctttttg ggtgatttcg gtggtgattt
      961 cgtacacgtt gtagccaacg ttcacgccgt cggccaccga ttgttcgtag gtgtattcgg
     1021 cgacgatgtt ttcgttaaaa aacccaaaag tgcgtttgtc gggcgtggcg gtcaggccaa
     1081 tcaggctggc gtcaaaataa tccagcacct gcttccacag gttgtagatg ctgcggtgac
     1141 attcgtcgat gatgatgaag tcaaaggttt ctactggcac agcagggttg taacgcacga
     1201 gtttttcttg cttggcggtt tgctgcactt cgttcagcga aacgtcttcg gcggattcgt
     1261 caatcggctc gccgctcaaa atggaataca tgcgctggat ggtgctgatg cacacttgcg
     1321 aatgcgggtc gatcgtgggc gagttcaggc gctggacgtt gtacagctcg gtaaatttgc
     1381 ggccatcgtc cggcggcgtg taggccatga attcctgatg cgcttgcttg cccaggttgc
     1441 gggtgtccac caaaaacaga atgcgcttgg cgccgccaaa tttcagcagg cgatataccg
     1501 aggtgatggc ggtgaaggtt ttgccggcgc ccgtggccat atgtaccagg gcgcggggtt
     1561 tgttcagcgc cagtgatttt tccagcccgg tgacggcgct gacttggcaa tcgcgcaggc
     1621 tgcgggtggc gagcgcaggc atttgcttgg ccaaacggca gcgtagcgtg tcaggctgcg
     1681 ccagccacgc agctaaggtt tccggc
//
LOCUS       DAOPYX010000440         1685 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_110252_length_1685_cov_5.852761, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000440 DAOPYX010000000
VERSION     DAOPYX010000440.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 1685)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1685)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1685
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_110252_length_1685_cov_5.852761"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1507)
                     /locus_tag="PKN55_14285"
     CDS             complement(<1..1507)
                     /locus_tag="PKN55_14285"
                     /inference="COORDINATES: protein motif:HMM:NF037827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX72462.1"
                     /translation="MSVTPSADPHLVFGFDLGSNSIGWAVLEENPAGQAQALRDMGVR
                     IFPKAVEEKTPTPKNHKRRQMRLARRVLERRARRRRRLQNYLILLGLLPDSVHDVRER
                     ERVLNTLGDPYALRAQALDQALSAHQLGRVIAHLGMRRGFQSNRKTLLSDMLDDPDVQ
                     DMLAELEQEDPQGHAASERERIKEEGQFKQAISALQADIHQAGCRTLGQYLASLPSAQ
                     RKRGRRIGRDMIRDELRAILAAQRPHHAGLDDAVCEEIERIIFHQRPLRWDRASIGAC
                     SLEKHRQRAAMARLEYQEFRLLQDINHLRYDRPCSHPDTGEILGLNLALTEQDRTRLL
                     DKLASQQDMSWSAVKKLLGLDTTITLNLEAGSKKGLPGNRTASKLANTLGADAWRALG
                     ASGQAELVEDLLKFEKKSALKKRLMTHWALPARTAIALATLELEPGHANLSLKAIRRL
                     LPFMRQGMRYDQAREAAGYGYSRTPSDGLERLPRPDDLRNPVVNRALHEFRR"
BASE COUNT          270 a          497 c          554 g          364 t
ORIGIN      
        1 cccggcgaaa ctcatgcagc gcgcgattca ccaccgggtt gcgcaggtca tccgggcgcg
       61 gcaggcgctc caggccgtcg ctgggcgtgc ggctatagcc atagccggcg gcttcgcgcg
      121 cttggtcgta gcgcatgccc tggcgcataa acggcagcag gcggcggatg gctttgagcg
      181 acaaattggc gtggccgggc tccagctcca gggtggccaa ggcaatggcg gtgcgcgccg
      241 gcaaggccca gtgagtcatc aggcgttttt taagcgcgga ttttttctcg aatttcagca
      301 ggtcttccac cagctcggct tgcccgctgg cgcccagcgc gcgccaggcg tcagcaccca
      361 gagtattggc cagtttgctg gcggtgcggt tgccgggcag gccttttttg ctgccggctt
      421 ccagattgag ggtgatggtt gtatccaggc ccagcagttt tttgacggct gaccagctca
      481 tgtcttgctg gctggccagt ttgtccagca ggcgggtgcg gtcttgctcg gtcagcgcca
      541 ggttcaggcc caaaatttcg cccgtgtccg ggtggctgca tgggcggtcg tagcgcaggt
      601 gattgatgtc ttgcaacaaa cggaattctt ggtattccag ccgggccatg gcggcgcgct
      661 ggcggtgttt ttccagcgag caggcgccaa tgctggcgcg atcccagcgc agcgggcgct
      721 gatgaaaaat aatccgctcg atttcttcgc atacggcgtc atccagcccg gcatggtgtg
      781 gacgctgggc ggccagaatg gcgcgcagct catcgcggat catgtcgcgg ccaatgcgcc
      841 ggccgcgttt gcgctgcgcg ctgggcaagc tggccaggta ttggcccaag gtgcggcagc
      901 cggcttggtg gatgtctgct tgcaaggcgc taatcgcttg cttgaactgc ccttcttctt
      961 tgatgcgttc gcgctcgctg gcggcgtggc cttgtgggtc ttcttgttcc agctcggcca
     1021 gcatgtcttg cacgtcgggg tcgtccagca tgtcggacag cagggttttg cggttggact
     1081 gaaacccccg gcgcatgccc aggtgggcaa tcacccgccc taattggtgg gcgcttagtg
     1141 cctggtccag cgcttgcgca cgcagggcgt aggggtcgcc cagtgtgttg agcacccgtt
     1201 cgcgctcgcg cacatcgtgc acgctatcgg gcagcagccc cagcaaaatc aaataatttt
     1261 gcaaacgccg ccgccgccgc gcgcgccgct ccagcacccg tcgggccagc cgcatttgtc
     1321 ggcgtttgtg gtttttgggg gtaggggttt tttcttcaac tgccttggga aagatgcgca
     1381 cgcccatatc acgcagggct tgcgcctggc cggccgggtt ttcttccagc acggcccagc
     1441 cgatggaatt gctgccaagg tcaaagccaa acaccaagtg cggatcggcg gagggggtga
     1501 cggacatgct gagctccaga acatgggatc agccgatagt atattgacat gccgtgccat
     1561 tggcattagc atcagttcat cagagtgtaa ctgactgatg cctgagaggc ttcatcacaa
     1621 gagatgaatt tctcgcagga tagccctttg tggcgccccc ggcctcccag cgaggccgtt
     1681 ttcac
//
LOCUS       DAOPYX010000441         1685 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_110257_length_1685_cov_4.796933, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000441 DAOPYX010000000
VERSION     DAOPYX010000441.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 1685)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1685)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1685
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_110257_length_1685_cov_4.796933"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..494)
                     /locus_tag="PKN55_14290"
     CDS             complement(<1..494)
                     /locus_tag="PKN55_14290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012695595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HNX72463.1"
                     /translation="MSFACLPISRVMLSSPGDASQLRVSHAELPPPAAGEIRVRHTAI
                     GVNYVDIYHRDGLYPLPVWPSPLGVEAAGWVEALGPGVSHLHIGQRVAYAGPPVGSYS
                     SARNLPAWLALPLPDTVADAAAASLLLRGITAHMLFTRVYRLQAGDTLLVHAAAGGLG
                     LVLT"
     gene            584..1501
                     /locus_tag="PKN55_14295"
     CDS             584..1501
                     /locus_tag="PKN55_14295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009240224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HNX72464.1"
                     /translation="MQKNTHEDGQTPRPLQWDDVRHFLALARLGSLSAAARQLAVEHS
                     TVARRVGALEKSLAVRLFDRLAKGWALTAEGEALLARAARMDDEAQAFARAALEVASL
                     QGVVRVSAPPAFTSHFLAPRLAARAQDWPQIDLELIGESRQANLVRGEADIALRMARP
                     EAPGLAGRQLAELGYGLYASAGHLARPSAHWCFLGDDALLRSPQQAWLDQVADGRRVV
                     LRGNDLTTLLHAARAGLGVAALPHFLAADDPVLRLAPAPACPVRRPLWLVLHPDVRRS
                     PRVRLVADVLTALIEDARQVLAGPMSASE"
BASE COUNT          275 a          514 c          596 g          300 t
ORIGIN      
        1 tgcgtcagca ccaggcccag cccgccagcg gcggcgtgca ccagcagggt gtcgcccgct
       61 tgcagccgat agacccgggt gaacagcatg tgtgcggtga tgccgcgcaa caacaggctg
      121 gcggcggcgg cgtcggcgac cgtatcgggc agcggcagcg ccagccaggc cggcagattg
      181 cgcgcgctgc tgtaactgcc caccggcggg ccggcgtacg ccacccgctg gccaatgtgc
      241 agatggctga cgcctgggcc cagcgcttcg acccaccctg cggcttctac gcccaggggg
      301 gatggccaga cgggcagcgg gtacaagcca tcgcggtgat aaatgtccac gtagttgacg
      361 ccgatggcgg tgtggcgcac gcggatttcg cccgctgcgg gcggcggcag ctccgcgtgg
      421 ctcacgcgca gctgtgaggc gtcgcccggc gaagacagca tcacgcggga aatgggcagg
      481 caggcaaagg acatggcaga agactccgtt cagggttgag attgtcagcc cagcttacgg
      541 tgtgcagaat ccatcgggaa ttcgcaaaat atggacattc actgtgcaaa aaaacacaca
      601 cgaagacggc cagacgccgc gcccgctgca atgggacgat gtgcgtcatt ttttggcgct
      661 ggcgcgcttg ggcagtttgt cggccgccgc ccgccaactg gcggtggagc attccaccgt
      721 ggctcgccgg gtgggcgcgc tggaaaaatc actggcggtg cgtttgtttg atcgattggc
      781 caaaggctgg gcgctgactg ccgagggcga ggccttgctg gcgcgtgctg cgcgcatgga
      841 tgacgaagcg caggcgtttg cccgcgcggc gctggaagtg gcgtcgctgc aaggcgtggt
      901 gcgggtgtcg gcgccgccgg cgttcaccag ccattttctg gcgcccaggc tggcggcgcg
      961 ggcgcaggac tggccgcaga ttgatctgga gctgattggc gaaagccgcc aggccaatct
     1021 ggtgcgcggc gaggcggata tcgccttgcg catggcgcgc ccggaagcgc cgggattggc
     1081 cgggcgtcag ctggccgagc tgggctatgg cttgtacgcc agcgccgggc atctggccag
     1141 accgtcagcg cactggtgtt ttttggggga tgatgccttg ttgcgctcac cacaacaggc
     1201 ttggctggat caagtggccg atgggcggcg ggtggtgttg cgtggcaatg atttgaccac
     1261 tctgttgcat gccgcacgcg caggtctcgg cgtggcagcc ttgccgcatt ttctggcggc
     1321 ggacgatcct gtgttgcggc tggcgcccgc gccggcctgc ccggtgcgcc gacccttgtg
     1381 gctggtgctg catccggatg tgcgccgctc accacgagtg cgtctggtgg cggatgtgct
     1441 gaccgcattg attgaagacg cccgccaggt gctggcaggc ccgatgtctg cttcagagtg
     1501 agccgtcaac aataacaggg cttgtccaca aaacgtttct gttgtggcgg catgtttggc
     1561 caggcaatga ccctgtacct tgtcctgcca tcctcacccc cttgtatccg ccgcctcgcg
     1621 cagtgcctcg ccaataaaaa tcagcagcac cagcgtgcca accagcacgc caaaggtgga
     1681 tagcg
//
LOCUS       DAOPYX010000442         1684 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_110441_length_1684_cov_1.717004, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000442 DAOPYX010000000
VERSION     DAOPYX010000442.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 1684)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1684)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1684
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_110441_length_1684_cov_1.717004"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            248..1339
                     /locus_tag="PKN55_14300"
     CDS             248..1339
                     /locus_tag="PKN55_14300"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNX72465.1"
                     /translation="MALFRVLHALRERGLTPLVVSIMAPQGEHFPDLGIETRSLNMPR
                     ARLNAQALRRLRDIVREFQPDIVQGWMYHSNVLAHLARTFASQQAGRRVPLAIAIRSS
                     MTLFAQEKRLTKLVIRLDAWLSRRADRVFYVSRLGERQHSAFGYAKHRAMIIPNGFNC
                     DTFQPMPQVRDAVRAELGLAPDTPLVGLVARWQPMKNHRGFLAAVGMLAERHPQAHFL
                     CVGRGCTAQDPAVLACTPESVRSRVHWLAERGDIPRLNAALDIAVNASLAEAFPNVVG
                     EAMACGIPMVVTDVGDSAWILDGCGRVVPPGDDQALSEAIDAMLSLPAQARQQLGARA
                     RERIMRDFSLQAVAEQYWREWSALVAASR"
     gene            1389..>1684
                     /locus_tag="PKN55_14305"
     CDS             1389..>1684
                     /locus_tag="PKN55_14305"
                     /inference="COORDINATES: protein motif:HMM:NF024868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNX72466.1"
                     /translation="MIVSIAVNAAWNVVNFRLGLIEALLAQGHQVHVLAPQGPEFTVL
                     ERLGCVTHVLDIDCKGTHPVKDFRLMLQLRACYARIKPDIALHYTIKPVIYGSLA"
BASE COUNT          298 a          439 c          547 g          400 t
ORIGIN      
        1 ggtgtcttct atggtaattt acggggcggt ggtgttcaac tatggcacgg cggcccgcta
       61 tcgttttcca tttgtcttgt tgtttgttat ttttgccgat cgtttgaccc agtcaccgaa
      121 aaagcatgct ggacagtagt ggttgccgtg gtaagtggcg cttaattgat caagaacaca
      181 aagtcatgat gaggtgtggg tgaagattct ggtggtgaca accgtcttgg cgacaggcgg
      241 cgcagaaatg gctttgttcc gggtgttgca cgctttgcgt gaacgtggat tgacgccttt
      301 ggtggtgtcg atcatggcgc cgcaaggcga gcattttccg gacctgggca tcgagactcg
      361 ctcattgaat atgccgcgag cgcgtctgaa cgcccaggcc ctgcgccgtt tgcgcgacat
      421 cgtgcgtgag ttccagccag atattgttca aggctggatg taccacagca atgtgctggc
      481 tcatctggcg cgaacctttg caagccagca ggctggccgg cgcgtgccgc tcgccatcgc
      541 cattcgcagc tcaatgacct tgtttgccca ggaaaaacgg ctgaccaaac tggtgatcag
      601 gctagatgcc tggctgtcgc gccgcgcaga ccgggtgttt tatgtgtcca gattagggga
      661 gcgtcagcac agcgcgtttg gctacgccaa acatcgcgcc atgatcatcc ccaacggctt
      721 taactgcgac acctttcagc cgatgccgca ggttcgcgac gctgtgcgcg cagaattggg
      781 cttggcgccg gatacgccgc tggttggcct ggtggcgcgt tggcagccga tgaaaaacca
      841 tcgcggcttt ctggcggcgg tcgggatgtt ggccgaacgt catccacagg cgcatttcct
      901 gtgcgtgggc cgtggctgta cggcgcaaga tcctgcggtg ctggcttgca cgcctgaatc
      961 ggttcgcagc cgggtgcatt ggttggctga gcgtggcgac attcctcggc tgaacgccgc
     1021 cctggatatt gctgtcaatg cctcactggc ggaagctttt cccaatgtgg tgggcgaggc
     1081 gatggcctgc ggcattccca tggtggtgac cgatgtgggc gactctgctt ggattctgga
     1141 tggatgcggc cgggtggttc cgcctggcga tgatcaggcg ctgagcgaag caatcgacgc
     1201 catgctgagt ctgccggctc aggcgcggca acagctgggc gcgcgcgcgc gtgagcggat
     1261 catgcgcgat ttttcactgc aggcggtggc cgagcagtat tggcgggaat ggtcggcgct
     1321 ggtcgccgct tcccgatgac agattaagtg ggcgtgctgc tggcgcaggg cccatggcgg
     1381 attcaagcat gattgtaagc atcgccgtga atgcggcatg gaatgtggtt aatttccggt
     1441 tgggcctgat tgaggcgctg ctggcgcaag gtcatcaagt gcatgtgctg gcgccgcaag
     1501 gcccagaatt taccgtgttg gaacgcctgg gctgtgtcac gcacgtccta gatatcgatt
     1561 gcaaaggcac tcatccagtc aaagattttc gtctgatgct gcaattgcgt gcttgctatg
     1621 cacgcatcaa gccagatatt gctttgcact acaccatcaa gccggtgatt tatggctcgt
     1681 tggc
//
LOCUS       DAOPYX010000443         1665 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_112306_length_1665_cov_9.916770, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000443 DAOPYX010000000
VERSION     DAOPYX010000443.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 1665)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1665)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1665
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_112306_length_1665_cov_9.916770"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..797)
                     /locus_tag="PKN55_14310"
     CDS             complement(<1..797)
                     /locus_tag="PKN55_14310"
                     /inference="COORDINATES: protein motif:HMM:NF024677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAFs-binding domain-containing protein"
                     /protein_id="HNX72467.1"
                     /translation="MTPLCFVLMPFGKKPTSDGRLVDFDAVYRQLIHPAICAAGLQAL
                     RADEEQVGGVIHKAMFERLILCEFAVADLTSANANVFYELGLRHAVRPHATQLLFAQG
                     WGQLPFDVNLLRALPYTLDASGAPDPAALAGEVAALSQKLRDARHAEADSPVYQLLEG
                     FPDIARLKTDVFRERVDYAETLKTALAQARASHQLAAIDQVAAQLGDVALADAGVVVD
                     VLLSYRALSAWAQMVDWVGKMSPPLRASTLVREQLGLALNRAGRSEEA"
     gene            complement(1294..>1665)
                     /locus_tag="PKN55_14315"
     CDS             complement(1294..>1665)
                     /locus_tag="PKN55_14315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HNX72468.1"
                     /translation="LKCYFMRDKVGEVFTGKISAVTSFGVFVLLDDVHIEGLLHISEL
                     GKDYFHFDMQRQAIVGEKSGMTFKLGDALAVKVVRADLESAKIDFVLSALPKAKPQDA
                     AEPAPRKRRSSKKPAEGNDAA"
BASE COUNT          330 a          520 c          507 g          308 t
ORIGIN      
        1 gcctcctcac tgcggccagc gcggttcagc gccaggccca gttgctcgcg caccagggtg
       61 ctggcgcgca agggcggcga catcttgcct acccaatcca ccatctgcgc ccaggcgctc
      121 agcgcccggt aggacagcaa cacatccacc accacgcccg cgtcggccag cgccacgtca
      181 cccagctggg cggcgacctg gtcaatcgcg gccaactgat ggctggcgcg cgcttgggcc
      241 agcgcggttt ttagcgtttc ggcgtaatcc acccgctcgc ggaacacatc ggttttcagc
      301 cgggcaatat ccggaaagcc ctccagcagc tgatacaccg ggctatcggc ctctgcatgg
      361 cgcgcgtcgc gcagtttttg gctgagcgcc gccacttcgc cggccagcgc agccgggtcg
      421 ggcgcgccgc tggcgtccag ggtatagggc aatgcgcgca gcaggttcac gtcaaacggc
      481 aactggcccc agccctgggc aaacagcagc tgggtggcgt gcgggcgcac ggcatggcgc
      541 aggcccagct cgtaaaacac attggcgttg gcgctggtca ggtcggccac ggcaaattcg
      601 cacagaatca agcgctcgaa catcgccttg tgaatcactc cgcccacctg ttcttcatca
      661 gcgcgcagcg cttgcaggcc agcggcgcag atggccggat ggatcagctg acggtacacc
      721 gcgtcaaaat ccaccagccg gccatcggag gtgggttttt taccaaacgg catcaacaca
      781 aagcaaagtg gggtcatggt gggcgctcgg ttggggagtc gtttatcctg acagcatggc
      841 caagctttgt cgatggcgcg cagcgcggcg ccccagcccg ccgcgtttgg ccagcgccgc
      901 tattgcagat aaaaccagtt ggccagcatg tcctggcagg cgtcatcggc cagccaggcg
      961 tccagccagt gggtgaatgt ggtgagcatg gcagcctccg ggtgggtggc cggcgcgggg
     1021 tgcgtgggcc tgatgaagcc agtataggcc ggggcttggc atggataaaa tgaattgatt
     1081 ttattttttc aatagcaaaa agctatcaat aaagaggcgc ggattgatgc agaaaaaccg
     1141 tgttctataa aataaaaacc cattaaaatc aaaacactga attcattcca gcgcggaaag
     1201 aacaattttc agcgtatcca cacaaccgcg ctcagcgtaa aaaaacacag cggcccgcag
     1261 gccgccatgt tcatctcgcg cggtggcgcc gcatcaggct gcgtcgttgc cctcggcggg
     1321 tttcttgctg ctgcgccttt tgcgcggggc cggttcggcg gcgtcttgcg gcttggcctt
     1381 gggcagggcg ctgagcacaa agtcgatctt ggcgctttcc aggtcggcgc gcaccacttt
     1441 caccgccagc gcatcgccca gcttgaaggt catgccgctc ttttcgccga caatcgcctg
     1501 gcgctgcatg tcgaagtgga agtaatcctt gcccagctca gagatatgca gcaggccttc
     1561 gatgtgcacg tcgtccagca gcacaaacac gccaaagctg gtgacggcgc tgatcttgcc
     1621 ggtgaacact tcgcccacct tgtcgcgcat gaagtagcac ttcag
//
LOCUS       DAOPYX010000444         1621 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_117262_length_1621_cov_9.471903, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000444 DAOPYX010000000
VERSION     DAOPYX010000444.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 1621)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1621)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1621
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_117262_length_1621_cov_9.471903"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..359)
                     /locus_tag="PKN55_14320"
     CDS             complement(<1..359)
                     /locus_tag="PKN55_14320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014088737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HNX72469.1"
                     /translation="MAFGSFDKGSNAPMAEINTTPLVDVMLVLLVVFIITAPLLTHAV
                     KVDLPKAAASAHEEKPEAIRLAINAQGELFWNDQPVAQDDLPARFAAATQANPQVELH
                     LRADREARYDAVARAMAA"
     gene            complement(340..1020)
                     /locus_tag="PKN55_14325"
     CDS             complement(340..1020)
                     /locus_tag="PKN55_14325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014088736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HNX72470.1"
                     /translation="MDLSLVFRQGDGVLMSVFLLLLAMSVTSWWLILRRSLSRWSVRQ
                     ANQQLSQAFWDAPDLDAAARLAEQHDAPLARIARAGLDGLGHYRRNQNHALGQACGID
                     EFLVRTIRMALARETARLESGLTWLATVGSVAPFVGLFGTVWGIYHALVGIGAAGQIT
                     LAAVSGPIGEALVATAAGLAAAIPAVVAYNAFTRVNRVDSQEMDGFAHDFHAQLIAAG
                     GQHGVREL"
     gene            complement(1028..>1621)
                     /locus_tag="PKN55_14330"
     CDS             complement(1028..>1621)
                     /locus_tag="PKN55_14330"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HNX72471.1"
                     /translation="ASAGAPVTPPTPLITEVVALAPEAAPTPPAPEPKPTQPQAKPEP
                     VKRQAAAPKPRPVSAPLTPSASPLAQGPSASKDADPTPPAPAAKPTAATGEPGPAKET
                     PPSFSAAYLSNPEPAYPAASLELGESGNVLLRVAVSAEGQPTSVEISRSSGYGRLDRA
                     ALSAVKRWRFTPARRGSEAVAGVVLVPINFQIKQQQG"
BASE COUNT          247 a          553 c          559 g          262 t
ORIGIN      
        1 gcggccatgg cgcgggccac agcgtcgtag cgcgcctcgc ggtcggcgcg caaatgcagc
       61 tcgacctgcg ggttggcctg ggtggcggcg gcaaagcgcg ctggcaaatc gtcttgcgcc
      121 accggctgat cgttccagaa cagctcgccc tgggcgttga tcgccaggcg gatggcttct
      181 ggtttttctt cgtgtgcgct ggccgccgcc ttgggcagat ccaccttcac cgcatgggtg
      241 agcagcggcg cggtgatgat gaacaccacc agcagcacca gcatcacgtc caccagcggc
      301 gtggtgttga tttccgccat cggcgcattg cttcccttgt caaagctccc gaacgccatg
      361 ctggcctcct gcggcaatca gttgcgcgtg aaaatcatgg gcaaagccgt ccatttcctg
      421 gctgtccacc cggttgacgc gggtgaaggc gttgtaagcc accacggccg gaatcgccgc
      481 cgccaggccg gcggcggtgg ccaccagcgc ctcgccaatc gggccggaca ccgccgccag
      541 ggtgatttgc ccggccgcac caatgcccac cagcgcgtga taaatgcccc acaccgtgcc
      601 aaacaggcca acaaacggcg ccaccgaacc cacggtggcc agccaggtca ggccggattc
      661 cagccgggcg gtttctcgcg ccagcgccat gcggatggtg cgcaccaaaa attcgtcaat
      721 gccgcaggcc tggcccagcg cgtggttctg gttgcggcga tagtggccca ggccatccag
      781 gccggcgcgg gcaatgcgcg ccagcggcgc gtcgtgctgt tcggccagac gggcggcggc
      841 gtccagatcc ggcgcatccc agaaggcctg cgacagctgc tgattggctt ggcgcacgct
      901 ccagcgcgac aggctgcggc gcaaaatcag ccaccagctg gtcactgaca tcgccagcaa
      961 gagcagaaac acactcatca gcacgccatc cccttggcga aataccagac tcagatccat
     1021 ggcttactta tccttgttgt tgcttgattt gaaaattgat gggcaccagc accacgccgg
     1081 ccaccgcctc gctgccgcgt cgcgccgggg taaagcgcca gcgcttgacc gccgacagcg
     1141 ccgcccgatc cagccggccg tagccgctgc tgcggctgat ctcgactgaa gtgggctggc
     1201 cttcggcgct gaccgccacc cgcagcagca cattgccgct ttcgcccagc tccagcgagg
     1261 cggccgggta ggccggctcg gggttggaca aatacgccgc cgagaacgat ggcggggttt
     1321 ccttggccgg gccgggctca cccgtggcgg ccgtgggttt ggcggcgggc gcgggcggcg
     1381 tggggtcggc gtctttgctg gcgctggggc cttgcgccag aggcgaggcg ctgggcgtca
     1441 gcggcgcgct caccggacgt ggcttgggcg ccgccgcctg gcgtttgacc ggctctggct
     1501 tggcttgcgg ctgagtgggc ttgggctcgg gcgcaggcgg cgtgggcgcc gcctcaggcg
     1561 ccagggcgac cacttcggtg atcagcggcg tgggcggcgt caccggcgcg ccagcgctgg
     1621 c
//
LOCUS       DAOPYX010000445         1621 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_117305_length_1621_cov_2.959770, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000445 DAOPYX010000000
VERSION     DAOPYX010000445.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 1621)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1621)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1621
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_117305_length_1621_cov_2.959770"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(122..1219)
                     /locus_tag="PKN55_14335"
     CDS             complement(122..1219)
                     /locus_tag="PKN55_14335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018746513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNX72472.1"
                     /translation="MIVFLTAFFASVLAVWLIIRSEHLHHHLTADSDLDGVQKFHVVA
                     VPRVGGLGILIGMLASASWLSLLLAAPPYQTWLLLMVASPAFFGGITEDMTKRVGVLP
                     RLLLTMLSAVAGYWFLGAALTRLDLPFIDRLLSDWWPVSLVVTAVAVGGVANAINLID
                     GYNGLAAMVAAGIALCLAFVGWMLHDSFMVVSCLSLAGALFGFLVWNFPFGRIFLGDG
                     GAYFVGFLLAELSVLLVARHPHVSAWFPMLLLFYPVFETLFTIFRRMSLHARSPGHPD
                     ACHLHQMLYKRAVRWHVGTRDPQHKTLRNALTAPYLWALWLVSAIPALLFWRFPALLM
                     LSCLGFAIVYVWLYMRLLRWRTPKWLILRRR"
     gene            complement(1337..>1621)
                     /locus_tag="PKN55_14340"
     CDS             complement(1337..>1621)
                     /locus_tag="PKN55_14340"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNX72473.1"
                     /translation="EGMPRTLLEAAAMGRPLVATDVPGCREVVQDGVNGYLCQVKDAG
                     ALAGAMERIITLSPSQRRALGDAGRHRVEERFAEGYVIDCYLRAISDMVR"
BASE COUNT          430 a          511 c          450 g          230 t
ORIGIN      
        1 tcgccggcca cgtatctggg caaggcagcg gagctggcca agcgcatctg agcgcgaaac
       61 gcccacggca gcatgcaaaa cgccttccct agggaaggcg tttttatttg ccagtcacag
      121 cttaacgtcg gcgcagaatc aaccacttgg gcgtgcgcca gcgcagcagg cgcatataca
      181 gccagacgta aacaatggca aagcccaggc agctgagcat cagcaaggcc ggaaagcgcc
      241 agaacagcag cgccggaatg gccgacacca accacagcgc ccacaaataa ggggcggtca
      301 gcgcattgcg cagggttttg tgctgcgggt cgcgcgtgcc gacatgccag cgcaccgccc
      361 gcttgtacag catctggtgc aagtggcagg catcagggtg gccgggcgaa cgcgcgtgca
      421 gactcatgcg gcggaaaatg gtgaacaggg tttcaaacac cggatagaac aacaacagca
      481 tgggaaacca ggccgacaca tgcgggtgac gggccaccag caacaccgag agttccgcca
      541 ataaaaagcc gacaaaatag gcgccgccat cgccgagaaa aatccggcca aacggaaaat
      601 tccacaccaa aaagccaaac aaggcgccag ccaatgacaa gcaggacacc accataaagc
      661 tgtcatgcaa catccagccg acaaacgcca gacataaagc aatgcccgcc gccaccatgg
      721 cggccaggcc gttgtagccg tcgatcagat tgatggcatt ggccacaccg ccgaccgcta
      781 ctgccgtcac taccagggac accggccacc agtccgacag caggcggtca ataaaaggca
      841 gatccagccg ggtcagcgct gcgcccagaa accagtagcc tgccacggca gacagcatgg
      901 tgagcaacag gcgcggcaat acgcccacac gcttggtcat atcctcggtg atgccgccga
      961 aaaatgccgg actggccacc atcagcagca gccaggtctg ataaggcggg gcggccagca
     1021 gcagcgacaa ccacgaggca gacgccagca taccgatcag gatgcccaag ccgcccacgc
     1081 gcggcaccgc cacgacgtgg aatttctgca cgccgtcaag atcggagtcg gcggtcaggt
     1141 gatgatgcag gtgttccgag cgaataatca gccacaccgc aagcacagag gcaaaaaagg
     1201 cagtcaggaa aacaatcatg ctttacggat catggatgag caattaggga gatgtggatg
     1261 gatagcgcca cattgcccaa gcacacatcc actcaggcat agcaatattg atgctttcag
     1321 catacagctt tctgcgttat cttaccatat ccgatattgc ccgcagataa caatcaatca
     1381 catagccttc agcaaagcgt tcttctacac ggtgacgtcc ggcatccccc agcgcgcgtc
     1441 gctgcgatgg agacaaggtg atgatcctct ccatggcccc ggccaatgcg ccggcgtcct
     1501 tgacctgaca cagatagcca ttgacgccat cttgcaccac ctcacggcag cccggcacat
     1561 ccgtggccac aagcggccgc cccatggcag cggcttccag cagggtgcgt ggcatgccct
     1621 c
//
LOCUS       DAOPYX010000446         1597 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_120026_length_1597_cov_10.746433, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000446 DAOPYX010000000
VERSION     DAOPYX010000446.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 1597)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1597)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1597
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_120026_length_1597_cov_10.746433"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..941)
                     /locus_tag="PKN55_14345"
     CDS             complement(<1..941)
                     /locus_tag="PKN55_14345"
                     /inference="COORDINATES: protein motif:HMM:NF012335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HNX72474.1"
                     /translation="MKAILYQQYGPPDVLQMADIAKPIPKPNEILIKTYATTVTSGDW
                     RVRSLTVPAGFGWMTRLVFGVSRPRQPILGSELAGVVESVGEAVSRFKVGDAVFAFSD
                     ARMGCYAEYKCMPQDGAVACKPLGLSYGEAAALSFGGTTALDFLRRAKLQRGESVLIN
                     GASGAVGAAAVQLARHFGAHVTAVCGTANMAWVRALGASQVIDYTQEDFTQNGQTYHV
                     IMDTVGTAPFSRCQASLRDGGRLLLVLAGLADMLHAPWASLTSGKKVIAGPVSVRAED
                     LPLLAELAATGAFKAVIDRRYRFEQMVEAHRYVDSGR"
     gene            complement(1215..1538)
                     /locus_tag="PKN55_14350"
     CDS             complement(1215..1538)
                     /locus_tag="PKN55_14350"
                     /inference="COORDINATES: protein motif:HMM:NF024949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNX72475.1"
                     /translation="MPSKNKPLTDAELDVYEAGRDLAADILQSIAEMKAGQVKPVDTS
                     AIEARMKTGLSQSQFARLLGVSVRTLQGWEQGRKHPSGAAKTLLAIAKMNPDALLEAS
                     KNLHS"
BASE COUNT          333 a          502 c          448 g          314 t
ORIGIN      
        1 ttgcgcccgc tatccacata gcgatgcgcc tcgaccatct gctcgaaccg atagcgccgg
       61 tcaatcaccg ctttgaacgc ccccgtcgcc gccagctcgg ccagcagggg caaatcttcc
      121 gcgcgcaccg aaaccgggcc ggcaatcacc ttcttgccgc tggtcagcga cgcccatggc
      181 gcgtgcagca tatcggccag ccccgccagc accagcaata aacgcccgcc gtctcgcaac
      241 gacgcttggc agcgagaaaa cggcgccgtg cccacggtgt ccatgatcac atggtaagtc
      301 tggccgttct gggtgaaatc ctcttgcgtg tagtcgatca cctggctggc gcccagcgcc
      361 ctgacccagg ccatattcgc cgtgccgcac accgccgtga catgcgcgcc aaaatgcctg
      421 gccagctgaa ccgccgccgc gcccaccgcg ccggacgcgc cattgatcag cacgctttcc
      481 ccacgctgaa gcttggcccg acgcagaaaa tccagcgccg tcgtgccgcc aaaagacaaa
      541 gcggccgctt cgccgtaaga caaaccaagc ggtttacacg ccaccgcgcc atcctgcggc
      601 atgcatttat actcggcgta acagcccatg cgggcgtcgc tgaacgcaaa caccgcatcc
      661 cccaccttga accggctgac tgcctccccc accgactcca ccaccccggc caactccgaa
      721 cccaaaatcg gctgcctggg ccgagatacg ccaaacacca agcgggtcat ccagccaaaa
      781 ccggccggca cggtcagact gcgcacccgc caatccccgg acgtgaccgt ggtggcatag
      841 gttttgatca ggatttcatt gggcttgggg atgggcttgg cgatgtccgc catctggagc
      901 acatcaggcg ggccgtattg ttggtagagg atggctttca tgaggcatgg cggtgttgag
      961 ttgggcgaga gtggagggtg atggatgcac tggaaaaacc gtcacttccg cgaaggtggg
     1021 agcccagagt tttgatttta ctggattttg tttttggccg acgtgatttt tctgaatgaa
     1081 tcaaggcatg cttagccggt cttggagccg cgacaccaaa cccggcgtag tagtcctaga
     1141 ggtgcgcggc cgcagacagt acgagcagca cgacaaggcc gcgcaacaag gccagggggt
     1201 gttgtgacca gcggttaaga atgcagattt tttgatgctt caagaagcgc atcagggttc
     1261 atcttggcaa tcgccagcaa ggtttttgcc gcaccgctgg ggtgtttgcg gccttgttcc
     1321 catccttgca gagtacgcac agaaacaccc agcaagcgcg caaattgtga ctgggataaa
     1381 ccggttttca tccgcgcctc aatcgctgag gtatccacag gtttgacctg cccggctttc
     1441 atctcagcaa tggattgcaa aatatcagca gccaagtcac ggccagcttc gtacacgtcc
     1501 aactcggcat cggtaagcgg cttatttttc gagggcatgt ttaatctcct tgagctttgg
     1561 gccagtcaga ttgtcggttt tggctttggc gtacagg
//
LOCUS       DAOPYX010000447         1585 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_121511_length_1585_cov_2.890196, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000447 DAOPYX010000000
VERSION     DAOPYX010000447.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 1585)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1585)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1585
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_121511_length_1585_cov_2.890196"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1422)
                     /locus_tag="PKN55_14355"
     CDS             complement(<1..1422)
                     /locus_tag="PKN55_14355"
                     /inference="COORDINATES: protein motif:HMM:NF025214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNX72476.1"
                     /translation="MAQYEEAEHSYRRGLKLQPKSANLHAYLADALFLQNRFADAIRH
                     ARQAIAQDPQHIVAHHNLGAALRSHSQLMEAETALRRALELAWNYPSAHANLANVLKD
                     QSHLEEAEKHLRIALEMTPNNRLIHSNLLFTGVYSHAITPEQLYADHCELGQLIARQA
                     GPRCTWSRTLAATPKRVGFVSADFFHHAVSYFVEPVWGGLRAQGMQVLAYYSGKTFDS
                     VSARLQKQCDVWRETAALSDDELAQQIIDDEVDILIDLSGHTAGGRLPVFAKQPAPVQ
                     VTWLGHPATTGLPGMHWRITDVHAEPPGMTEHLNTERLYRMPEVFCCYQPSASPDPFA
                     GLPADQDGRFTFGCFNNFAKVTPPVIACWGEILRQTPHARLLLEVHGAQDAAFVQDIR
                     QRFVTAGAQATQIDILPRQANQQYVLYRQVDLALDPFPCCGGTTSCDSLWMGVPFVTL
                     AGRSFVSRMGVTLLHNTGLDELIA"
     gene            1396..>1585
                     /locus_tag="PKN55_14360"
     CDS             1396..>1585
                     /locus_tag="PKN55_14360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX72477.1"
                     /translation="MFGFFVLRHLGKRHPQIAEDFHILGGQLGRLAHGLHAFGVTPKL
                     DQHSAQGFPAQHMARVMFC"
BASE COUNT          354 a          456 c          448 g          327 t
ORIGIN      
        1 ggctatcaat tcatccagcc ccgtattgtg cagtaaggtc acccccatgc gcgaaacaaa
       61 actgcgtccg gccaaggtga caaatggcac gcccatccac aggctatcgc agctggtggt
      121 gccgccacag catggaaaag gatccagtgc caggtccacc tggcgataca acacatattg
      181 ctgatttgcc tgtcgtggca agatatcgat ctgtgttgcc tgcgcacctg ccgtcacgaa
      241 tcgctggcga atatcctgga caaaggcggc atcctgagcg ccatgtacct ccagcagcag
      301 ccgggcatgc ggcgtctgcc gcaaaatttc tccccagcat gcaatgaccg gcggcgtcac
      361 tttggcaaag ttattgaaac acccaaaggt aaaacgtcca tcctggtctg ctggcaagcc
      421 ggcaaaagga tcaggagacg ccgagggttg ataacagcaa aacacctcgg gcatgcgata
      481 cagccgttca gtattcaggt gctcggtcat gccaggcggc tcggcgtgca catcggtgat
      541 gcgccagtgc atgcctggca ggccagtggt ggccgggtgg ccaagccagg tcacttgtac
      601 aggcgccggt tgtttggcaa acacaggcaa gcggccacca gccgtatggc cagacaaatc
      661 aatcaagata tccacctcat catcaatgat ttgctgagcc aattcgtcat cgctcaaagc
      721 agctgtctcg cgccagacat cgcactgttt ttgcaggcgt gcactgacgc tgtcgaaggt
      781 tttgccactg tagtaagcca gcacctgcat gccctgcgca cgcaggccgc cccagaccgg
      841 ttcgacaaaa taactgaccg catgatgaaa aaagtctgct gagacaaaac ccactcgctt
      901 gggcgtagcc gcaagcgtgc gcgaccaggt acaacgcggc ccagcttgtc tggcgatcaa
      961 ttgtcccagt tcgcaatgat cggcatacaa ctgctcgggg gtaatggcgt gactgtacac
     1021 tccagtaaat aagaggttgc tgtgaatcag tcgattgttc ggcgtcatct ccagcgcaat
     1081 gcgcaagtgt ttttcagcct cttccagatg tgactgatct ttcagcacat tggccagatt
     1141 ggcgtgagcc gaaggatagt tccaggccag ctccagcgca cggcgcaagg cggtttctgc
     1201 ttccatcaac tggctgtgtg agcgcaacgc cgcacccaga ttatggtggg cgacaatatg
     1261 ttgagggtct tgggcaatgg cttggcgcgc gtggcgaatg gcgtcagcaa agcgattttg
     1321 caagaacagc gcatccgcca gataggcgtg cagattggcg gatttgggct gtagcttcaa
     1381 gccgcgccga taactgtgtt cggcttcttc gtactgcgcc atctgggtaa gcgccacccc
     1441 cagattgctg aggatttcca catccttggg ggccagttgg gccgcctggc gcatggcctg
     1501 cacgccttcg gcgttacgcc caagctggat cagcacagcg cccagggttt tccagcccaa
     1561 cacatggcga gggtaatgtt ctgtc
//
LOCUS       DAOPYX010000448         1583 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_121821_length_1583_cov_2.221204, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000448 DAOPYX010000000
VERSION     DAOPYX010000448.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 1583)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1583)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1583
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_121821_length_1583_cov_2.221204"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            182..976
                     /locus_tag="PKN55_14365"
     CDS             182..976
                     /locus_tag="PKN55_14365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019924794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNX72478.1"
                     /translation="MNNALRAVWAFRGFVLSNVKREFQTKYRNSLLGMAWMVIHPLSM
                     ILVYTVIFSHMMKSKLPGIEGGFAYSIYLCAGILTWGLFVEIVSRAQNIFIDNANLIK
                     KLNFPKLCLPLIVVLNALLNFAIISALFLAFLLVAGLFPGWVALAALPVLVIQVLFSI
                     GLGVTLGVFNVFFRDVGQLFGVVINFWFWFTPIVYPVSALPPWLSPWLVLNPMAPLIG
                     AYQDIFVRGQLPAWGSLAPILVLALVLCGLAAVLFRRHAGEMVDEL"
     gene            976..>1583
                     /locus_tag="PKN55_14370"
     CDS             976..>1583
                     /locus_tag="PKN55_14370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019924793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNX72479.1"
                     /translation="MGAIQVTGLGKAYRQYASRWGRLAEWLLPGARPRHQEKWVLHDI
                     SFAVAPGEAVGIVGINGAGKSTLLKLITGTSQPTRGTVSLQGRVAALLELGMGFHPDF
                     TGRQNAYTAGQLIGLSAEDIHALMPEIEAFAEIGDYIDMPVRVYSSGMQMRLAFSVAT
                     CKRPDILIVDEALSVGDAYFQHKSFNRISEFAKQGTTLLIVS"
BASE COUNT          281 a          456 c          485 g          361 t
ORIGIN      
        1 gcggcgagta cctgggcgaa gacgacatcg tgcgttttgc cgacaattac ggccgcagct
       61 gaaatcgggc ccagacccga agcatcatca aaaataaaaa gcagcccgct tgcccggtct
      121 tgctgacggc tggacgctat gcacttctgg ccaccagcca caacccacag aaatgtaagt
      181 tatgaacaac gcgctgcgtg ccgtatgggc gtttcggggc tttgtcctgt caaatgtcaa
      241 aagagaattc cagaccaagt atcgcaactc gctcttgggc atggcatgga tggtgattca
      301 tccgctgtcg atgatcctgg tctacacggt gattttttcc cacatgatga aatccaagct
      361 gcccggcatc gagggtggct ttgcctacag tatttacctg tgcgccggca tcctcacctg
      421 gggcttgttt gtcgagattg tcagccgggc gcagaacatc tttatcgaca acgccaacct
      481 gatcaaaaag ctcaactttc ccaagttgtg cctgcccttg atcgtggtgc tgaatgccct
      541 gctcaacttt gccatcatca gtgcgctgtt tctggctttt ttgctggtgg ccgggctgtt
      601 tccgggctgg gtggcgctgg cggcgctgcc ggtgctggtg attcaggtgc tgttttccat
      661 tggcctgggc gtcacccttg gggtgttcaa tgtgtttttc cgcgacgtcg gccagctgtt
      721 cggcgtggtg attaatttct ggttctggtt cacccccatc gtctatccgg tcagcgcttt
      781 gccgccgtgg ctgtcgccgt ggctggtgct gaacccgatg gcgccgctga ttggcgcgta
      841 tcaggatatt ttcgtgcgcg ggcagctgcc ggcctggggc agcctggcgc ccatcctggt
      901 gctggcgctg gtgttgtgtg gcctggcggc ggttttgttc cgccgtcatg ccggtgaaat
      961 ggtggatgaa ctgtaatggg cgcgattcaa gtcacagggt tgggcaaggc ctaccgacaa
     1021 tatgcttcgc gctggggacg gctggccgaa tggctgctgc ccggcgcgcg gccgcgccat
     1081 caagaaaaat gggtgctgca cgacatcagc tttgccgtgg cccctggcga ggcagtgggc
     1141 attgtcggca tcaacggcgc cggcaagagc accctgctca agctgatcac tggcaccagc
     1201 cagcccacgc gcggcacggt cagcctgcaa ggccgggtgg cggcgctgct ggagctgggc
     1261 atgggctttc acccggattt caccggccga cagaacgctt acacggcagg ccaactgatt
     1321 ggcctgagcg ccgaggacat ccacgccctg atgcccgaaa tcgaggcctt tgccgaaatt
     1381 ggcgattaca tcgacatgcc ggtgcgggtg tactccagcg gcatgcagat gcgcctggcc
     1441 ttcagtgtgg ccacctgcaa acgcccggac atcctgattg tggacgaggc gctgtcggtg
     1501 ggcgatgcct attttcagca caagagcttt aaccgcatca gcgaattcgc caagcaaggc
     1561 accaccttgc tgattgtctc gca
//
LOCUS       DAOPYX010000449         1572 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_123086_length_1572_cov_9.111404, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000449 DAOPYX010000000
VERSION     DAOPYX010000449.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 1572)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1572)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1572
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_123086_length_1572_cov_9.111404"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..479
                     /locus_tag="PKN55_14375"
     CDS             <1..479
                     /locus_tag="PKN55_14375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008953003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNX72480.1"
                     /translation="LALNAAIEAARAGETGRGFAVVADEVRRLAERTGQSTTEINQIV
                     ANIRTVTDSAVNSMHKVSDSSAQSELHLADTAASLNDILQASQSVEQMMRSIADTNVQ
                     QSATAHDLAERTVGISTRIEASTQEIGQAGRLIAELAGKAEDLQTMIRRFDTGDKR"
     gene            complement(547..1140)
                     /gene="wrbA"
                     /locus_tag="PKN55_14380"
     CDS             complement(547..1140)
                     /gene="wrbA"
                     /locus_tag="PKN55_14380"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012698418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003955 - NAD(P)H dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HNX72481.1"
                     /translation="MQEILVLYYSQHGAVKQLAQLIARGVESVPGCQARLRTVPKVST
                     VCEATAPAIPDAGAPYVEPVDLVECAGLALGSPTRFGNMAAAMKYFLDGSTGLWLNGA
                     LAGKPACVFTSSSSQHGGQESTLLSMMLPLLHHGMLLVGLPYSEPALSATQTGGTPYG
                     VSHVAGPNNDRPISDDERKLAIAQGKRLAEVALKLRG"
     gene            1251..>1572
                     /locus_tag="PKN55_14385"
     CDS             1251..>1572
                     /locus_tag="PKN55_14385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008952235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX72482.1"
                     /translation="MRRCRPGQCGFAHAQPFAMLFAARVSRLLPPALARLLGFAGFVV
                     RRALREERCVQVAASLAFTSLLSLVPLVTLALIVLSAFPVFADYSARIKLFLLSNLVP
                     DFAGK"
BASE COUNT          255 a          500 c          523 g          294 t
ORIGIN      
        1 tcttggcgct gaacgccgcc atcgaagccg cccgcgccgg cgaaaccggc cggggttttg
       61 ccgtggtggc cgatgaagtg cgccgcttgg ctgaacgcac cgggcagagc acgacggaaa
      121 tcaatcagat tgtcgccaac atccgcacgg tgaccgattc ggcggtgaac agcatgcaca
      181 aggtcagcga cagctcggca caaagcgaac tgcatctggc cgacaccgcc gccagcctga
      241 acgacatctt gcaggccagt cagtcggtgg agcagatgat gcgcagcatt gccgacacca
      301 atgtgcagca gtcggccacc gcgcacgatc tggctgagcg cacggtgggc atcagcaccc
      361 ggattgaggc ctccacccag gaaatcggcc aggccggacg cttgattgcc gagctggctg
      421 gcaaggcgga ggatttgcaa accatgattc gccgctttga taccggcgat aagcgctaag
      481 cgccgctgac aaaacctgcc tggcgtggcg gtgtggcaat gtcaggcaga tttgctggag
      541 tgatgctcag ccgcgcagct tcagcgccac ttccgccagg cgcttgccct gggcgatggc
      601 cagtttgcgt tcgtcgtcgg aaatcggccg gtcgttattg ggcccggcca catggctgac
      661 gccatagggc gtgccgccgg tctgggtggc ggacagcgcc ggttcagaat acggcaggcc
      721 caccagcagc atgccgtggt gcaggagcgg cagcatcatc gacagcagag tgctttcctg
      781 gccgccgtgc tggctggacg agctggtgaa cacacaggcc ggcttgccgg ccagcgcgcc
      841 gttcagccac aaaccggtgc tgccatccag aaaatacttc attgccgccg ccatattgcc
      901 aaagcgggtg gggctgccca gcgccaggcc ggcgcattcc accagatcca ccggttcgac
      961 atagggcgcg ccagcgtccg ggatggccgg ggcggtggct tcgcacacgg tggagacttt
     1021 gggcacggtg cgcagccgcg cctggcagcc gggcacggat tccacccctc gggcgatcaa
     1081 ttgcgccagt tgcttcaccg cgccatgctg gctgtagtac agcaccagaa tttcctgcat
     1141 tgtgttctcc gtcagcactg ttgggcgtcg cgcccttctt cagggcgctt gagcgcgcag
     1201 gggcgcgacg cgaatgccgc tattgtaacc gcagcgcgcg cggcgcttgt gtgcgccgct
     1261 gtcggcccgg acaatgcggg tttgcccacg cccagccctt tgccatgctg tttgccgccc
     1321 gtgtgtcccg tttgttgccg cccgccctgg cccgcctgct gggctttgcc ggttttgtgg
     1381 tgcgccgcgc gctgcgcgaa gagcgctgcg tgcaggtggc cgccagcctg gcgttcacca
     1441 gcttgctgtc gctggtgccg ctggtgacgc tggcgctgat tgtgctgtcg gcgtttccgg
     1501 tgtttgccga ttacagtgcg cgcatcaagc tgtttttgct gagcaatctg gtgccggatt
     1561 ttgccggcaa ga
//
LOCUS       DAOPYX010000450         1568 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_123553_length_1568_cov_8.449438, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000450 DAOPYX010000000
VERSION     DAOPYX010000450.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 1568)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1568)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1568
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_123553_length_1568_cov_8.449438"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..389
                     /locus_tag="PKN55_14390"
     CDS             <1..389
                     /locus_tag="PKN55_14390"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HNX72483.1"
                     /translation="APRHPRELAASDCIGLLGNGPVWWRFVEQGRAFSVASGGRLEFN
                     HIAPAIDACVAGLGLGAFLSYQIAGHLAAGRLRPVLTEFEPAPRPVQLIYPHARLLPA
                     RVRVALDWLEARLRQRLAGLDALEAD"
     gene            504..1448
                     /locus_tag="PKN55_14395"
     CDS             504..1448
                     /locus_tag="PKN55_14395"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HNX72484.1"
                     /translation="MPPSAALPIEPRPEQFSHAGARWLAATRPAFLSVSLAGAVLGVA
                     ASLPAVSWGLVGWALLAVLLLQAGVNVFNDYYDHDNGCDAANTERVFPFTGGSRFIQN
                     GVMSPAQMRALALALFAAVALIGLGLVWVRGWPLLCLGLAGAALGWAYSAPPCRLNSR
                     GLGELTVALTFYGVPLGMAWVASGQINPALACASLSQALLITALLYINQFPDRRADTL
                     AGKRHWVARLSLPAAARGYTWLTSLAYGVLVLGVWHQLLPLTALCGLATLPASLLASA
                     QLRQHADQPAALRPAIIATLLAANLHPLCLALGLAWPV"
BASE COUNT          197 a          528 c          555 g          288 t
ORIGIN      
        1 gcgcgccgcg ccacccgcgc gaacttgccg ccagcgactg catcgggcta ttgggcaatg
       61 ggccggtgtg gtggcgcttt gtcgagcagg gccgcgcctt tagcgtggcc tcgggcgggc
      121 ggctggagtt caatcacatc gccccggcca tcgacgcttg tgtggccggg ctggggctgg
      181 gggcgttttt gtcttaccag attgccgggc acctggccgc cggccggctg cgcccggtgc
      241 tgaccgagtt tgagccggca ccgcgcccgg tgcagctgat ctacccgcac gcccggctgt
      301 tgccggcgcg ggtgcgggtg gcgctggatt ggctggaggc acggctgcgc cagcgcctgg
      361 ccgggcttga tgccctggaa gctgattgag catcaagcag cccaatggcg cgacaaagcc
      421 cctagcgtgc catgcgcaac agccccgcac acttgcaaaa gccctatact gtgcgccgat
      481 gatttccccg accggagcct gtcatgccgc cttctgccgc cttgccgatt gaaccccgcc
      541 ccgagcagtt ttcccacgcc ggcgcccgct ggctggccgc cacccggccg gcgtttctca
      601 gcgtgtcgct ggccggcgca gtgctgggcg tggcggccag cctgccggcg gtgagttggg
      661 gcctggtggg ctgggcgctg ctggcggtgt tgctgctgca agccggggtg aatgtgttca
      721 acgactatta cgaccacgac aatggctgcg acgccgccaa taccgagcgg gtgtttccgt
      781 ttaccggcgg cagccgcttt atccagaacg gggtgatgag cccggcgcag atgcgcgcgc
      841 tggcgctggc cttgtttgcc gctgtggcgc tgatcggcct gggcctggtg tgggtgcgcg
      901 gctggccgct attgtgtctg gggctggccg gcgcggcgct gggctgggcg tattcggcgc
      961 cgccctgccg gctgaacagc cggggcctgg gcgagctgac cgtggcgctg accttttacg
     1021 gcgtgccgct gggcatggcc tgggtggcca gcggccagat caaccccgcg ctggcctgcg
     1081 ccagcctgag tcaggctttg ttgattaccg cgctgctgta tatcaatcag tttcccgacc
     1141 gccgcgccga caccctggcc ggcaagcgcc attgggtggc gcggctttcc ttgccggcgg
     1201 cggcgcgcgg ctacacctgg ctgacttcgc tggcctatgg cgtgctggtg ctgggggtgt
     1261 ggcatcaatt actgccgctg accgcgctgt gcggcctggc caccctgccg gccagcctgc
     1321 tggccagcgc ccagttgcgc cagcacgccg accagccagc ggcgctgcgc ccggcgatta
     1381 tcgccaccct gctggcagcc aatctgcatc cgctgtgcct ggcgctgggg ctggcgtggc
     1441 cggtctgagc gccgccagca ccgctgaaca tccaaaaaat agcagatagc ccccgttgga
     1501 tgccgatggc tccgttaaga gccgctaaca aaaccccttg atgcatgcag gcgcgacccg
     1561 atctggtg
//
LOCUS       DAOPYX010000451         1567 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_123691_length_1567_cov_10.473545, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000451 DAOPYX010000000
VERSION     DAOPYX010000451.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 1567)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1567)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1567
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_123691_length_1567_cov_10.473545"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1101
                     /locus_tag="PKN55_14400"
     CDS             <1..1101
                     /locus_tag="PKN55_14400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014087256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HNX72485.1"
                     /translation="KYLVNADGSLGARNDLVCASIEHKLGIHASPTAVMVFGENDGAI
                     GYLVGEPHKGLAYMFTMMNHARLNVGMEGVAISERAFQQALAYAKDRVQGKPMGDSSG
                     QTRPILYHPDVRRMLMDMKSRIEAMRALAYQTAAHMDYAHHHPDEAVRREHQARVELL
                     TPVVKGWCTETSINVTSTGIQVHGGMGFIEETGAAQHFRDARITTIYEGTTAIQANDL
                     IGRKVAREGGSSLAVLLADIDATREQLLASGNAQLSAIGAALARGAGALAEARDWLLA
                     HYASQPAQAAAGAVAFLKLTGVVAGGWMMARAAQVATQLLADGDSDTVFLNAKLATAR
                     YYADQVLPEAALYAAQITQGAASALALDEEGF"
     gene            1136..>1567
                     /locus_tag="PKN55_14405"
     CDS             1136..>1567
                     /locus_tag="PKN55_14405"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014087257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNX72486.1"
                     /translation="MSERDSMQYDVVIVGGGPAGLSAAIRLKQLAEQQGREVSVCLLE
                     KGSEVGAHILSGAVIDPIALNELIPDWKEQGAPLNTPVSEDRFLFLTETEATQFPEKL
                     MPPLLNNHGNYIISLGNLCRWLATQAEALGVEIYPGFAAAEV"
BASE COUNT          291 a          523 c          517 g          236 t
ORIGIN      
        1 aaatacctgg tcaacgccga cggctcgctg ggcgcgcgca atgatctggt gtgcgcctcg
       61 atcgaacaca agctgggcat tcacgccagc cccactgcgg tgatggtgtt tggcgagaac
      121 gacggcgcca tcggctatct ggtgggcgag ccgcacaagg gcctggcgta tatgttcacc
      181 atgatgaacc acgcccgcct gaacgtgggc atggaaggcg tggcgatttc cgagcgcgct
      241 ttccagcagg cgctggccta cgccaaggat cgcgtgcagg gcaagccgat gggcgacagc
      301 agcggccaga cccggccgat tctgtaccac ccggacgtgc gccgcatgct gatggacatg
      361 aaatcgcgca tcgaagccat gcgcgcgctg gcctaccaga ccgccgcgca catggactac
      421 gcccaccatc acccggacga agccgtgcgc cgcgagcatc aggcccgcgt tgagctgctc
      481 accccggtgg tgaagggctg gtgcacggaa acctcgatca atgtcacctc caccggcatt
      541 caggtgcacg gcggcatggg ctttatcgaa gaaaccggcg ccgcccagca tttccgcgac
      601 gcccgcatca ccaccattta cgaaggcacc acggccattc aggccaatga cctgatcggc
      661 cgcaaagtgg cgcgcgaagg cggcagctcg ctggcggtgc tgctggccga catcgacgcc
      721 acccgcgaac aactgctggc cagcggcaac gcccagctga gcgccattgg cgcggcgctg
      781 gcgcgcggcg ctggcgcgct ggccgaagcc cgcgactggc tgctggcgca ctacgccagc
      841 cagccggcgc aagccgctgc cggcgcggtg gccttcctca agctgactgg cgtggtggcc
      901 ggcggctgga tgatggcgcg cgccgcgcaa gtggccaccc aattgctggc cgatggcgac
      961 agcgacacgg tgttcctgaa cgccaagctg gccaccgccc gctactacgc cgaccaggtg
     1021 ctgccggaag ccgcgctgta cgccgcgcaa atcacccagg gcgccgcctc ggcgctggcg
     1081 ctggacgagg agggtttctg atgtcagccc ggcttgcccg cgtgggaggt gcgcaatgag
     1141 cgaacgcgat tccatgcaat acgacgtggt gatcgttggc ggcggcccgg ccgggctgtc
     1201 ggcggcgatt cgcctcaagc aactggccga acagcagggg cgcgaagtca gcgtctgcct
     1261 gctggaaaaa ggctcggaag tcggcgcgca tattctgtct ggcgcggtga tcgacccgat
     1321 tgcgctcaat gagctgatcc cggactggaa agaacaaggc gcgccgctca acaccccggt
     1381 gagcgaagac cgctttttgt tcctcaccga aaccgaagcg acgcagtttc cggaaaaact
     1441 gatgccgccg ctgctcaata accacggcaa ctacatcatc agcctgggca atctgtgccg
     1501 ctggctggcg acgcaggccg aggcgctggg cgtggaaatc taccccggct ttgccgccgc
     1561 cgaagtg
//
LOCUS       DAOPYX010000452         1550 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_125796_length_1550_cov_13.556522, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000452 DAOPYX010000000
VERSION     DAOPYX010000452.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 1550)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1550)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1550
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_125796_length_1550_cov_13.556522"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..431
                     /locus_tag="PKN55_14410"
     CDS             <1..431
                     /locus_tag="PKN55_14410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HNX72487.1"
                     /translation="DRLDTLGKVRAAGLSVCCGGIVGMKETRRDRAGLIAQLANLSPQ
                     PESVPMNSLVKVEGTPLDDADALDWTEFVRTIAVARLTLPHSYIRLSAGRRDMPEAVQ
                     ALCFMAGANSIFYGDKLLTTGNPEVDRDRALMSKLDLLPL"
     gene            433..>1550
                     /gene="bioF"
                     /locus_tag="PKN55_14415"
     CDS             433..>1550
                     /gene="bioF"
                     /locus_tag="PKN55_14415"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018748058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HNX72488.1"
                     /translation="MRLIAQLQDKLAALDGDHRRRRCPVLDSPQGPHVRIDGRDYLAF
                     ASNDYLGLANHPALVAAARDGALRWGVGGGASHLVAGHSAAHAALEQALADWLRLPAA
                     LLFSSGYQANLAVITALVGRGDAVFADRLNHASLNDACLLSRAEFKRFAHHDLAQLER
                     LLAASTAPTKLIAVDAVYSMDGDCAPLAALLALAERFDAWLYVDDAHGFGVLGEGRGS
                     VVEAGVHSERLIQMVTLGKAAGVAGAAVAAHAVVIDWLVNSARPFIFTTSTSPLLAHT
                     VQASLRLMADEPERRAALRARMAELKHQLAGLPWTLLPSDTPIQPLLIGGNAEALAVS
                     AGLRARGIWVPAIRPPTVPPGSARLRIALSAAHQPQDIV"
BASE COUNT          213 a          541 c          557 g          239 t
ORIGIN      
        1 aagaccgcct ggacaccctg ggcaaggtgc gcgccgccgg gctgagcgtg tgctgtggcg
       61 gcattgtcgg catgaaagaa acccgccgcg accgcgccgg cctgattgcc cagctggcca
      121 acctcagccc gcagccggaa tcggtgccga tgaacagcct ggtgaaggta gaaggcacgc
      181 cgctggacga cgccgacgcg ctggactgga ccgaatttgt gcgcaccatc gccgtggccc
      241 ggctgaccct gccgcacagc tatatccgcc tgtccgccgg ccgccgcgac atgccggaag
      301 cggtgcaggc gctgtgcttc atggccgggg ccaattcgat tttttacggc gacaagctgc
      361 tgaccaccgg caacccggaa gtggaccgcg accgcgcgct gatgagcaag ctcgacctgt
      421 tgccgctgtg atatgcggct gatcgcgcaa ctgcaagaca agctggccgc gctggacggc
      481 gaccaccgcc gccgacgctg cccggtgctg gacagcccgc aaggcccgca cgtgcgcatc
      541 gacggccgcg actacctggc ctttgccagc aatgactatc tgggcctggc caatcacccg
      601 gcgctggtgg ccgccgcccg cgacggcgcc ttgcgctggg gcgtgggcgg cggcgcttcg
      661 cacctggtgg ccgggcacag cgccgcccac gccgcgctgg agcaggcgct ggccgactgg
      721 ctgcgcctgc cggcggcgtt gttgttttcc agcggctatc aggccaatct ggcggtgatc
      781 accgccctgg tgggccgggg cgacgcggtg tttgccgacc ggctgaacca cgcctcgctg
      841 aacgacgcct gcctgttgtc gcgcgccgag ttcaaacgct ttgcccacca tgacctggcc
      901 cagctggagc gcctgctggc ggccagcacg gcgccgacca agctgattgc cgtggatgcg
      961 gtgtacagca tggatggcga ctgcgcgccg ctggcggcgc tgctggcgct ggccgagcgc
     1021 tttgacgcct ggctgtatgt ggacgacgcc cacggctttg gcgtgctggg cgaggggcgc
     1081 ggcagtgtgg tcgaggccgg cgtgcacagc gagcggctga tccagatggt gacgctgggc
     1141 aaggcggccg gggtggccgg cgcggcggtg gcggcgcatg ccgtggtgat tgactggctg
     1201 gtgaacagcg cacggccgtt tatcttcacc accagcactt cgccgctgct ggcgcacacc
     1261 gtacaggcca gcctgcggct gatggccgac gagcccgagc gccgcgccgc gctgcgtgcc
     1321 cgcatggccg agctcaagca ccagttggcg ggcttgccgt ggaccctgct gccgtcggac
     1381 acgccgattc agccgctgct gattggcggc aacgccgagg cgctggcggt gtcggccggg
     1441 ctgcgcgcgc gcggcatctg ggtgccggcc attcgcccgc ccaccgtgcc gcccggctcg
     1501 gcgcggctgc gcattgccct ctccgccgcc caccagccgc aggatattgt
//
LOCUS       DAOPYX010000453         1540 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_127038_length_1540_cov_11.778451, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000453 DAOPYX010000000
VERSION     DAOPYX010000453.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 1540)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1540)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1540
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_127038_length_1540_cov_11.778451"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(100..576)
                     /locus_tag="PKN55_14420"
     CDS             complement(100..576)
                     /locus_tag="PKN55_14420"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019922991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HNX72489.1"
                     /translation="MSADTAPFIHPLADVHSQQIGELSKVWQFCVVLAGARIGRNCNI
                     CSHCFIENDVWIGDEVTVKNGVYLYDGIVLEDQVFIGPNATFTNDKSPRSKVYPEAFL
                     KTTIKRGASIGGGAVILPGVTVGEFAMVGAGAVVTRDVPAYATVVGNPARVLRQGA"
     gene            complement(608..1072)
                     /locus_tag="PKN55_14425"
     CDS             complement(608..1072)
                     /locus_tag="PKN55_14425"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNX72490.1"
                     /translation="MTADLSWRVCRFSELTVHSLYAVLRLRDQVFVVEQDSVYGDIDN
                     LDQDAWHVLACAADGALLAYARVLAPGVKDPLAACIGRVVVAPAARGLGLGGALLDRA
                     LALAGEQYPGAPWVLSAQCDKQGVYASRGFVAEGEPYDDGGIDHVTMRRAAL"
     gene            1189..>1540
                     /locus_tag="PKN55_14430"
     CDS             1189..>1540
                     /locus_tag="PKN55_14430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007343097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HNX72491.1"
                     /translation="MNDLLASSAVPADGDTLTLDAYAERAYLEYAMSVVKGRALPEVA
                     DGQKPVQRRILYAMKEMGLTAGAKHVKSARVVGEILGKYHPHGDSSAYEAMVRLAQDF
                     VMRYPLIDGQGNFGS"
BASE COUNT          320 a          517 c          456 g          247 t
ORIGIN      
        1 aatggctggg cacgccgcgt aaaacgctgt acgacaaact gcaacgccac ggcatccagc
       61 ccgaggggtt tcggcgctga aggcaggcag tcgcgcaatt caagcgccct ggcgcagcac
      121 ccgcgccggg ttgcccacca cggtggcata ggccggcaca tcacgagtga ccaccgcgcc
      181 cgcgcccacc atggcgaact cccccacggt cacgccgggc aaaatcaccg cgccgccgcc
      241 aatcgaagca ccgcgtttaa tggtggtttt caagaatgcc tcagggtaaa ccttggagcg
      301 cggagattta tcgttggtga aggtggcgtt ggggccgata aacacctgat cttccagcac
      361 aatgccgtcg tacagataaa cgccgttttt caccgtgact tcatcgccaa tccacacatc
      421 gttttcaata aagcaatgcg agcaaatatt gcaattgcgg ccaatgcgcg cgccggccag
      481 caccacgcaa aactgccaca ccttgctaag ctcgccaatt tgctggctgt gcacatcggc
      541 cagcggatga ataaaaggag cggtgtcagc actcatcggc gattctccaa tcacagaaca
      601 gacttcatca tagcgcagcg cggcgcatgg tcacatggtc aatgccgcca tcgtcatacg
      661 gctcgccctc ggccacaaag ccccggctgg cgtacacccc ctgcttgtcg cattgcgccg
      721 acagcaccca aggcgcgccc ggatactgct cgccggccag cgccagcgcc cgatccagca
      781 gcgcgccgcc caggcccagc ccgcgcgcgg ccggcgccac caccacccgg ccaatgcagg
      841 cggccagcgg gtcttttacc cctggcgcca gcacgcgggc gtaggccagc aaggcgccgt
      901 cggcggcgca ggccagcaca tgccaggcgt cctgatccag gttgtcgata tcgccgtaca
      961 ccgaatcctg ctcgaccaca aacacctgat cgcgcaggcg cagcacggcg tacaggctgt
     1021 gcacggtaag ttcagaaaat cggcaaacgc gccagctcag gtcagcagtc atggtgggcc
     1081 tcatggagat tgtggcgggg tgcggctaca atcacgcggt aaccgcccgg aacccggcct
     1141 ggccggggcc gtcatcgtat catcgccccc ctgtgaagtc ctgagttcat gaacgacttg
     1201 ttagcctctt ccgccgtccc agccgacggc gacaccctga cgctggatgc ttacgccgag
     1261 cgcgcttatc tggaatacgc gatgagcgtg gtcaaaggcc gcgcgctgcc agaggtcgcc
     1321 gacgggcaaa agccggtgca gcggcgcatt ctgtacgcca tgaaagaaat gggcctgacc
     1381 gccggcgcca agcatgtgaa gtcggcgcgc gtggtgggcg agattcttgg caaataccac
     1441 ccgcacggcg acagctcggc gtacgaagcc atggtgcggc tggcgcagga ttttgtcatg
     1501 cgctacccgc tgatcgacgg ccagggcaat tttggcagcc
//
LOCUS       DAOPYX010000454         1525 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_128987_length_1525_cov_9.906803, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000454 DAOPYX010000000
VERSION     DAOPYX010000454.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 1525)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1525)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1525
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_128987_length_1525_cov_9.906803"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            94..1278
                     /locus_tag="PKN55_14435"
     CDS             94..1278
                     /locus_tag="PKN55_14435"
                     /inference="COORDINATES: protein motif:HMM:NF017727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
                     /protein_id="HNX72492.1"
                     /translation="MPPALTLAPHRALFTTGALATLLPMLWWLLELLGRASGIPLLDH
                     PVPAMLAHGQWMLYGVFPPFMAGFIFTAGPRWLGVNGPGARAFAPLAAAWLLGHLATL
                     AGLVHNWQWLYQSGQGLFAAGMLGLCGVWLGVIRQSRQPDRRHAWLVAAGFALGAAGA
                     LAGLGWALTGRAELWSAMRGLALWGFLLPVFLTVAHRMLPFFSANVLQPYQPWRPDGL
                     LSAFWAGSLGHLLLSLLPWAWPLSLWDAGFAALLGYTSWRWGVRRSLINPLLAMLHLS
                     FAWAALALALSALTPWLTLGAAATHALGIGLFASLMMGFVSRVSLGHSGQPLRAAPWL
                     WRLYLALHGLALLRLASDWLPPFAAIGHLLTAAGWLLLWGIWCARFVPLYCQPRADGQ
                     PG"
BASE COUNT          166 a          546 c          505 g          308 t
ORIGIN      
        1 gcgccggccg gatcgcttga tgcaaatcat gggcacctgc gctgccggcc gccgacaatg
       61 cacactcccc cgattttgtg aatccgaccc atcatgcccc ccgccctcac ccttgccccg
      121 caccgggcgc tgttcaccac tggcgcgctg gccacgctct tgcccatgct ctggtggttg
      181 ctggaactgc taggccgcgc cagcggcatt cccctgctgg atcatcccgt gccggccatg
      241 ctggcgcacg gccaatggat gctgtacggg gtttttccgc cgttcatggc cggctttatt
      301 tttaccgctg gcccgcgctg gctgggcgtg aatggccctg gcgcacgcgc ctttgccccc
      361 ttggccgccg cctggctgct cggccatctg gccaccctgg ccgggctggt gcacaactgg
      421 cagtggcttt accaaagcgg ccagggtttg tttgccgccg gcatgctggg attgtgcggg
      481 gtatggctgg gggtgatccg gcaaagccgc cagccagacc gccgccacgc ctggctggtg
      541 gccgccggct ttgccctggg cgcagcgggc gcgctggccg gcctgggctg ggcgctgact
      601 ggccgcgccg agctgtggag cgccatgcgc gggctggcgc tgtgggggtt tttattgccg
      661 gtgtttctca ccgtggcgca ccgcatgctg ccgtttttca gcgccaatgt gctgcaaccc
      721 tatcaaccct ggcggccgga tgggctgttg tcggcgttct gggccggcag cctgggccat
      781 ctgctgctga gcctgctgcc gtgggcctgg ccgctgtcgc tgtgggacgc cggctttgcc
      841 gcgctgctgg gctacaccag ctggcgctgg ggcgtgcgcc gcagcctgat caacccgctg
      901 ctggccatgc tgcatctgtc gtttgcctgg gcggcgctgg cgctggcgct gtcggcgctc
      961 accccctggc tgacgctggg cgccgccgcc acccacgcgc tgggcatcgg cctgttcgcc
     1021 agcctgatga tgggctttgt cagccgcgtg tcgctgggcc actccggcca accgctgcgc
     1081 gccgcgccct ggctgtggcg gctctacctg gccttgcacg gcctggccct gctgcggctg
     1141 gccagcgact ggctgccgcc gttcgccgcc atcggccacc tgctcaccgc cgccggctgg
     1201 ctgctgctgt ggggcatctg gtgcgcgcgc tttgtgccgc tgtactgcca gccgcgcgcg
     1261 gatgggcagc cgggctaagc gccgctcaca aaaccctcct ggcgttgttg cccgaccttg
     1321 ccgtactcct tgtactgtct gcggtcgggc gcctagccag gcgcgctgcg ctggggtttt
     1381 gttagcaact ctaagcgcgg ctctttagga agcagaagcc accgccccaa gggcggcctc
     1441 accgcctcgc gactcacccc ccaccagcac agccccagca cgcacattgc gtattattac
     1501 catggtattt tcacgaattg attgg
//
LOCUS       DAOPYX010000455         1508 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_131412_length_1508_cov_9.293875, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000455 DAOPYX010000000
VERSION     DAOPYX010000455.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 1508)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1508)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1508
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_131412_length_1508_cov_9.293875"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..301
                     /locus_tag="PKN55_14440"
     CDS             <1..301
                     /locus_tag="PKN55_14440"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HNX72493.1"
                     /translation="NPQARGRVTEFDGFAQRYSKPGVPEAVAAYAALAEAHGLSPAQL
                     ALKFVYSRWFVASTIIGATSLAQLEENLNAWDAPWSEALEQAVADIRLRWFNPAP"
     gene            438..1472
                     /locus_tag="PKN55_14445"
     CDS             438..1472
                     /locus_tag="PKN55_14445"
                     /inference="COORDINATES: protein motif:HMM:NF024772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNX72494.1"
                     /translation="MSFTRLLRPHAVRAACAALALSLSGLALAEPIKVGYLPVTGHAK
                     FFVAKEQGLFAQEGLDVELVEFQNSADGLNAVVAGKLDVGAFGTTAPVAHISKGANLK
                     IIGGIMGGDAAVITTPENASKYNKISALRGKKIATVRMASGDAVLRGALKDAGIRWKT
                     DVQIFELKNPPAVIEAVKSGQVDAGVVWGPHDLRAEDQGLKVVIHSTDLQPGHPCCRL
                     VVTDTRLKDQATWEKFLRAILKAEKFATENKQQTIDSIAKYVKLDPKLLWRGFYSPYL
                     DQSSDPNVKGVKHFADVMVNSEFIPTKPDLNAAIDSQLYAKVLAQLARENPNDPFWKK
                     LQKEYKQKDA"
BASE COUNT          309 a          474 c          473 g          252 t
ORIGIN      
        1 caacccgcag gcgcgcggcc gggtgaccga gtttgacggc tttgcccagc gctacagcaa
       61 gccaggcgtg cccgaggcgg tggccgccta cgccgcgctg gccgaggcgc acggcttgag
      121 cccggcgcag ctggcgctga agtttgttta ttcgcgctgg tttgtcgcca gcaccatcat
      181 cggcgccacc tcgctggccc agctggaaga aaacctcaac gcctgggatg cgccgtggag
      241 cgaggcgctg gaacaggcgg tggcggatat ccgcctgcgc tggttcaacc cggcgccgta
      301 agccggcgac gcctggcggg tttggtgagc cgcgcttatc tgaaaaagcc atatcgttgt
      361 gcggattcct tgtttagcct ggccggccgg cttgggtaca gtgcggcgca tccccattgc
      421 acttcaggag cgctgtcatg tcctttaccc gtttgctgcg tccgcacgcc gtgcgcgccg
      481 cctgcgccgc gctggcgctg agcctgtccg gcctggcgct ggccgagccg atcaaggtcg
      541 gctatctgcc ggtcaccggc cacgccaagt tttttgtcgc caaagagcag ggcctgttcg
      601 cccaggaagg gctggatgtc gagctggtgg aattccagaa ctcggccgac ggcctgaatg
      661 ccgtggtggc cggcaagctg gacgtgggcg cgtttggcac caccgccccg gtggcgcata
      721 tttccaaagg cgccaacctg aagatcatcg gcggcatcat gggtggcgac gcagcagtga
      781 tcactacgcc ggaaaacgcc agtaaataca ataaaatcag cgcgctgcgc ggcaaaaaaa
      841 tcgccaccgt gcgcatggcc tcgggggacg ccgtgctgcg cggcgcgctc aaggacgccg
      901 gcatccgctg gaaaaccgat gtgcagattt tcgagctgaa aaacccgcca gcggtgattg
      961 aagcggtgaa atccggccag gtggacgctg gcgtggtgtg gggcccgcat gacctgcgcg
     1021 ccgaagacca gggcctgaaa gtggtgatcc actccaccga cctgcaaccc ggccacccgt
     1081 gctgccggct ggtggtcacc gacacccggc tgaaggatca ggccacctgg gagaaattcc
     1141 tgcgcgccat tctcaaggcg gaaaaattcg ccaccgagaa caaacaacaa accatcgatt
     1201 cgatcgccaa atacgtcaag ctcgacccga aactgctgtg gcgcgggttt tactcaccgt
     1261 atctggacca atccagcgac cccaacgtca agggcgtcaa acactttgcc gacgtgatgg
     1321 tgaacagcga attcatcccc accaagccgg acctgaacgc cgccatcgac agccaactgt
     1381 acgccaaagt gctggcccag ctggcgcggg aaaaccccaa cgacccgttc tggaaaaagc
     1441 tgcaaaaaga gtacaagcaa aaggacgcat gatgagccag ggactgattc tggaaggcgt
     1501 gcactttg
//
LOCUS       DAOPYX010000456         1488 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_134185_length_1488_cov_9.598046, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000456 DAOPYX010000000
VERSION     DAOPYX010000456.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 1488)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1488)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1488
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_134185_length_1488_cov_9.598046"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..283)
                     /locus_tag="PKN55_14450"
     CDS             complement(<1..283)
                     /locus_tag="PKN55_14450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX72495.1"
                     /translation="MVKVGFIVEGESEKLVVESPMFQQWLHDHDCTLVTPVIDAKGGG
                     NLLPQHIEPFIQRLSHAGVERILVLTDLEDEAHPDLVRQRVAHPRIQCIF"
     gene            complement(277..1395)
                     /locus_tag="PKN55_14455"
     CDS             complement(277..1395)
                     /locus_tag="PKN55_14455"
                     /inference="COORDINATES: protein motif:HMM:NF024700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNX72496.1"
                     /translation="MMKIHKLHIKGFKSVADLTLENVSPFLVLAGANGSGKSNITDAL
                     AFLGDVVKQGATQARTRFGGFAQIHCFKYRKEQRTTMQFALVLELDGAVYDYHLILRT
                     LDAAPQIDETLRVNGQKIIDRPANGELHVRLGSASAMQVLPDYPKDMTALMLLGSLPL
                     YEFLTNIKVFRIDPLAAKEPSRATTDASELDEYGRNVASVLSRLEAEPEFREQVQEWL
                     ELIVPGMESISTETQRLDSSTVITFKEAGTKTRFPAKLVSDGTLYVLCMLTAVLSRAE
                     RRGITIIEEPERGIHPKAIGELVQLMRENASAEHPIFLTTHSESIVRQLNTAELYLVS
                     KVDGKTKVSPAELAGVDKTQIPLDTAWLANLFDGGLPW"
BASE COUNT          309 a          451 c          396 g          332 t
ORIGIN      
        1 caaatatgca ttggatgcgc ggatgcgcca cccgctgccg caccaggtcg gggtgcgctt
       61 cgtcttccag gtcggtgagg acaagaatcc gttcaacacc ggcatggctc aggcgttgaa
      121 taaatggctc aatatgctgg ggcagcaaat tgccgcctcc tttggcgtcg atgaccggcg
      181 tgaccaaggt gcaatcatgg tcgtgcagcc actgctgaaa catcggcgac tccaccacca
      241 atttttcgct ctcgccttcc acgataaacc cgactttcac catggcaagc ccccgtcaaa
      301 taaattggcc agccaggcgg tatccagcgg aatctgggtt ttatccacac cggccaattc
      361 ggcggggctt accttggttt tgccgtccac tttactgacc agatacagct cagcggtatt
      421 gagctgacga acaatcgatt cactgtgcgt ggtcaaaaaa atcgggtgct cggcggaggc
      481 gttttcccgc atcagctgca ccaattcgcc aatcgccttg gggtgaatgc cccgctcggg
      541 ttcttcgatg atggtgatgc cacggcgctc ggcgcgactg agcaccgccg tcaacataca
      601 caacacatac agcgtgccat ccgacaccag cttggccggg aagcgggttt ttgtgccggc
      661 ctccttgaag gtgatcaccg tggagctatc caggcgctga gtttcggtgg aaatgctttc
      721 catgccgggc acgatcagtt ctagccattc ttgcacctgc tcacgaaact caggctcggc
      781 ctcaagccgg gacagcaccg acgccacatt gcggccatat tcatccagtt cgctggcgtc
      841 cgtggtggcg cggctgggtt ctttggccgc cagcggatca atgcgaaaca ccttgatatt
      901 ggtcagaaat tcatacagcg gcaaactacc cagcaacatc aatgcggtca tgtctttggg
      961 gtagtccggc aacacctgca tggcagacgc actgcccagc cgcacatgca gctcgccatt
     1021 ggctgggcgg tcgatgattt tttgcccgtt tacgcgcagt gtttcgtcaa tctgcggcgc
     1081 ggcgtccaag gtgcgcaaga tcaggtgata gtcgtacacc gcgccgtcca gctccagcac
     1141 caaggcaaat tgcatggtgg tgcgctgttc tttgcgatat ttgaagcagt ggatctgggc
     1201 aaaaccgcca aaccgggttc tggcttgtgt cgccccctgt ttgaccacgt cccccaaaaa
     1261 cgccaaggca tccgtgatat tgctcttgcc cgagccattg gcgccagcca ggactaaaaa
     1321 cggcgagaca ttctcaagcg ttaaatccgc cacgctcttg aagcccttga tgtgcaattt
     1381 atgaatcttc atcatcctgc ctttgtgtgc ccctttagcg ccagagtgtg ccgggtttgc
     1441 gccctcaccc aatctcccga tacccatcac aggcgcgctg aaacacct
//
LOCUS       DAOPYX010000457         1446 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_140404_length_1446_cov_13.629763, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000457 DAOPYX010000000
VERSION     DAOPYX010000457.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 1446)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1446)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1446
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_140404_length_1446_cov_13.629763"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..434
                     /locus_tag="PKN55_14460"
     CDS             <1..434
                     /locus_tag="PKN55_14460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012696661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNX72497.1"
                     /translation="AILWFGLGNPPSFFLIAIGAFFPVLMNTIAGVRHVDGIYLRAAR
                     NLGVNQLTLFTRVILPAATPYILAGVRIGIGTAFIVVIVSEMIAVNDGLGFRILEARE
                     FMWSDKIIAGMITIGLLGLAIDTAVSRLNNHLLRWHRGLEH"
     gene            436..1227
                     /locus_tag="PKN55_14465"
     CDS             436..1227
                     /locus_tag="PKN55_14465"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012696662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNX72498.1"
                     /translation="MSQILINNVQKVFTTPGGPVVALKDIQLTIQPGEFVCLLGPSGC
                     GKSTLLNAVAGFQPPSAGEITIEGKKILSPGPDRGMVFQEYALFPWMTVAQNIAFGLE
                     VQKKSRSDIAQTVNQLLDLLHLKDFRDRYPKDLSGGMRQRVAIARVLALDSPIMLMDE
                     PFGALDALTRRNLQDELLRIWEKLGKTILFVTHSIEESIYLADRIVVMTYRPGTVKRD
                     QRVDMPRPRDPSSAAFNALKRELGALVMEEQQRHANAEMKLATVD"
BASE COUNT          274 a          433 c          442 g          297 t
ORIGIN      
        1 tggccatctt gtggtttggc ctgggcaacc cgccgtcgtt ctttttgatc gccattggcg
       61 cgtttttccc ggtgctgatg aacaccatcg ctggcgtgcg ccacgtggac ggcatttatc
      121 tgcgcgcggc gcgcaatctg ggcgtcaacc aactgaccct gttcacccgc gtcatcctgc
      181 cggcggccac gccgtatatc ctggccggcg tgcgcattgg catcggcacg gcgtttattg
      241 tggtgattgt ctccgagatg atcgccgtca acgatggcct gggtttccgc attctggaag
      301 cgcgtgagtt tatgtggtcg gacaagatca tcgccggcat gatcaccatc ggcctcttgg
      361 gcctggccat cgacaccgcc gtcagccgcc tgaacaacca tctgctgcgc tggcatcgcg
      421 gcctggagca ctaagatgag ccagattctg atcaataatg tgcaaaaagt gttcaccacg
      481 ccgggtggcc cggtggtggc gctgaaagac attcaactga ccatccagcc cggcgagttt
      541 gtctgcctgc tggggccgtc cggctgcggc aaatccaccc tgctgaacgc cgtggccggt
      601 tttcagccgc cgtcggcggg cgaaatcacc atcgagggca aaaaaatcct cagccccggc
      661 ccggatcgcg gcatggtgtt tcaggagtac gcgctgtttc cgtggatgac cgtggcgcaa
      721 aacatcgcct ttggcctgga ggtgcaaaaa aaatcgcgca gcgacatcgc ccagacggtg
      781 aatcagctgc tggatttgct gcacctgaaa gactttcgcg accgctaccc gaaagacctc
      841 tccggcggca tgcgccagcg ggtggcgatt gcccgcgtgc tggcgctgga ttcgccgatc
      901 atgctgatgg acgaaccgtt tggcgcgctg gacgcgctga cccggcgcaa tctgcaggac
      961 gagctgctgc gcatctggga gaaactgggc aagaccatcc tgtttgtcac ccactcgatt
     1021 gaagagtcga tctacctggc cgaccgcatc gtggtgatga cctaccgccc cggcacggtc
     1081 aaacgcgacc agagggtgga catgccgcgc ccacgcgacc catcgtccgc cgcgttcaac
     1141 gccctcaagc gcgagctggg cgcgttggtg atggaggagc aacagcgcca tgccaatgcc
     1201 gagatgaagc tggcgacggt ggattgagca cggttagcaa agccttgaag tgttgctgaa
     1261 gttcgacgct tgctcctgtg ggggcaagcg tcgcaaggcg ctgtgctgcg ggtaggccta
     1321 ccttaacggt cgttgcgcaa ttgcgcgcca tcccatactt tgcaaagcaa gcccttttcg
     1381 gtgatatatt attgatatac acgaaagaag tcggctcagc ggcatggtga gtcggaaatc
     1441 accgct
//
LOCUS       DAOPYX010000458         1442 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_141064_length_1442_cov_7.995674, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000458 DAOPYX010000000
VERSION     DAOPYX010000458.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 1442)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1442)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1442
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_141064_length_1442_cov_7.995674"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..971
                     /locus_tag="PKN55_14470"
     CDS             <1..971
                     /locus_tag="PKN55_14470"
                     /inference="COORDINATES: protein motif:HMM:NF013056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HNX72499.1"
                     /translation="GVLFMLVSITRLREGFVNGIPLSLKQAISAGVGLFIALIALQQA
                     GVVVANPDTLVGLGDLHKPQALLALAGFALIVTLERWRVPGAILLGILAITALSAALG
                     LSEFNGVFAAPPSLAPTWLQLDVAGALAPGVLSVVLGFFLMSLTETSGTLIAVAQRGP
                     FLDAEGKLPRLNRALLADSAAISVGALLGTSSTTSYLESSAGIAAGGRTGLTAVTVAG
                     LFLASLFFAPLAAAVPPWATAPALVYVAMLMLKGFALLNWDDITEAAPALLCALTMAF
                     TFSIADGIAFGAIAFVTLNLAAGRRQQIGWPVALIASVFVLKYVLS"
     gene            complement(1086..>1442)
                     /locus_tag="PKN55_14475"
     CDS             complement(1086..>1442)
                     /locus_tag="PKN55_14475"
                     /inference="COORDINATES: protein motif:HMM:NF024845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-TFIIB domain-containing protein"
                     /protein_id="HNX72500.1"
                     /translation="HTRKTTMKCPVCANVTLVMSERQGIEIDYCPQCRGVWLDRGELD
                     KIIERSRADAPPTHAASGLFAQPAAPAPQPYPQPQHTQRPSYPEPHRHGDSHHAHSHD
                     HDSRKRKKSLWEELFD"
BASE COUNT          172 a          441 c          518 g          311 t
ORIGIN      
        1 cgggcgtgtt gtttatgctg gtcagcatca cccggctgcg cgaaggcttt gtcaacggca
       61 ttccgctgtc gctgaaacag gccatctcgg ccggggtggg gttgtttatc gccctgattg
      121 ccctgcaaca ggcgggggtg gtggtggcca acccggatac gctggtgggc ctgggggatt
      181 tgcacaagcc gcaggcgctg ctggcgctgg ccggctttgc cctgattgtc accctggagc
      241 gctggcgtgt gccgggggcg attttgctgg ggattctggc catcaccgcg ctgtcggcgg
      301 cgctggggct gagtgaattc aacggcgtgt ttgccgcgcc gccgtcgctg gcgcccacct
      361 ggctgcaact ggacgtggcc ggcgcgctgg cgccgggggt gttgagcgtg gtgctgggct
      421 tctttttgat gagcctcacc gaaacctcgg gcaccttgat cgccgtggcc cagcgcgggc
      481 cgtttctgga cgccgagggc aagctgccgc gcctgaatcg cgccttgctg gcggacagcg
      541 ccgccatcag cgtgggcgcc ttgctgggca cgtccagcac cacttcttac ctggaaagca
      601 gcgccggcat cgccgcgggc gggcgcaccg ggctaacggc ggtgaccgtg gcgggtttgt
      661 ttttggccag tctgtttttt gccccgctgg ccgccgccgt gccgccgtgg gccaccgcgc
      721 cggccttggt gtatgtggcc atgttgatgc tcaagggctt tgccctgctc aattgggacg
      781 acatcaccga agccgcgccc gcgctgctgt gcgcgctgac catggcgttt accttctcga
      841 ttgccgacgg catcgccttt ggcgccattg cctttgtcac cctcaacctg gccgccggcc
      901 gccgccagca gattggctgg ccggtggcgc tgattgcctc ggtgtttgtg ctcaagtatg
      961 tgctgagctg aggccgcgcg gctcggcgcc gcgcacaaac cccgcctggc attgttgcac
     1021 gcacctgtac tgtctacggt cgtgcgcctg gccaggatcg cttcgctgag ctttgtgagc
     1081 cgcgcttagt cgaacagctc ttcccacagc gacttcttgc gcttgcgcga gtcatggtcg
     1141 tgggaatggg cgtgatggct atcgccgtgg cgatggggct cggggtagga cgggcgctgg
     1201 gtatgttgcg gctgggggta gggttgcggc gcaggcgccg ctggctgggc aaacaggccg
     1261 ctggccgcgt gggtcggcgg ggcgtcggcg cgcgagcgct cgatgatttt gtccagctcg
     1321 ccgcgatcca gccagacgcc gcgacattgc gggcagtagt cgatttcgat gccctggcgt
     1381 tcactcatga ccagggtgac gttggcgcag accggacatt tcatggttgt tttccgtgtg
     1441 tg
//
LOCUS       DAOPYX010000459         1435 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_142082_length_1435_cov_10.686957, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000459 DAOPYX010000000
VERSION     DAOPYX010000459.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 1435)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1435)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1435
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_142082_length_1435_cov_10.686957"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(15..458)
                     /locus_tag="PKN55_14480"
     CDS             complement(15..458)
                     /locus_tag="PKN55_14480"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HNX72501.1"
                     /translation="MLTLLPSLPDVSGHLLRAGIPVTAQRLAIARVLLPLPVHLSAEQ
                     VQQCASHYTSGLSRATVYNTLKLFTARGLLRELAVEPGKTMFDSNPLPHHHLYNSATG
                     ELTDIPADTLRLSGAPVLPDGLELEQIEVIVRVRPREDGQPAPRN"
     gene            complement(713..>1435)
                     /locus_tag="PKN55_14485"
     CDS             complement(713..>1435)
                     /locus_tag="PKN55_14485"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HNX72502.1"
                     /translation="PTVLNSRLNLAQFWDGRAKDLKEQAGGPIANPKEMAFSHALAVD
                     VLRSIPQYVNEFKKVYGEPAIDIGKVTTAIAAFEDTLVTPDSRFDQWLKGNKKALSAQ
                     ELRGYQTFKNSGCVACHNGPNVGGNSFQKMGQVEPYKTSNPAEGRVAVTGKDADRFTF
                     KVPTLRNVELTYPYFHDGEAATLAQAVDVMGRLQLGKKFTEQEIGDVVSFLKTLTGKQ
                     PDFKLPILPPSTDSTPRPRPFD"
BASE COUNT          257 a          437 c          464 g          277 t
ORIGIN      
        1 gctgtgagcg gcgcttaatt gcgtggggcg ggctggccgt cctctcgcgg acgcacccgc
       61 acaatcactt cgatttgctc cagctccaag ccgtcgggca gcaccggcgc accagacagg
      121 cgcagggtgt cggccgggat gtcggtcagc tcgccggtgg cgctgttata caaatggtga
      181 tgtggcaggg gattggaatc aaacatggtt tttcccggct ccaccgccag ttcgcgcagc
      241 aggccgcggg cggtgaacag cttgagggtg ttgtacaccg tggcgcgcga caagccgctg
      301 gtgtagtggc tggcgcactg ttgcacctgc tcggcgctca ggtgcaccgg cagcggcaac
      361 agcacacggg caatggccag ccgttgcgcg gtcaccggaa taccggcgcg caacaaatga
      421 ccggacacat ccggcaggga aggcagcaaa gtgagcatgg caacgcctct tgcacaacag
      481 aaccatgaaa cggcgcacga tggcgcgcca gccgggtgct gtcaagccca tgcccacacg
      541 ccatccggat ggcatccgac ccacggcgtc cagaacgtat cacgctcggg cactgtgggt
      601 cgctcaggcg ctgtcgattt gcagcacaca gcgcacggcc atcctgcccc gctgcccagc
      661 cgccacccag gcaaaatgac gcagcagcgg caggcagggt gcgtgcggcg tttcagtcaa
      721 acggacgcgg gcgcggggtg ctgtcggtgg acggtggcag aatcggcagt ttgaaatccg
      781 gttgcttgcc ggtcagcgtt ttcaagaagc tcaccacatc gccgatttcc tgctcggtaa
      841 acttcttgcc cagttgcaga cggcccatca cgtccaccgc ctgcgccagg gtggcggctt
      901 cgccatcgtg gaagtacggg taggtcagtt cgacattgcg cagcgtgggc accttgaagg
      961 taaaccgatc ggcatcctta ccagtgactg ctacgcgccc ctcggccggg ttgctggttt
     1021 tgtacggctc gacctggccc attttctgga aggaattgcc gccaacgttc gggccattgt
     1081 gacaggccac acaaccgctg ttcttgaaag tttggtagcc gcgcagctcc tgggcgctca
     1141 gcgccttttt attgcccttc agccattggt caaagcggct gtccggggtg accagggtgt
     1201 cttcaaaggc ggcaatcgcc gtggtgacct tgccgatgtc gatggcgggt tcgccgtaga
     1261 cctttttgaa ttcgttgaca tactgcggaa tcgagcgcag cacatccacc gccagcgcgt
     1321 ggctgaaggc catttctttg gggttggcga tcgggccgcc ggcttgttct ttcaggtctt
     1381 tggcgcggcc atcccagaac tgggccaggt tcagccgcga attgagcacg gtggg
//
LOCUS       DAOPYX010000460         1429 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_143010_length_1429_cov_8.783843, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000460 DAOPYX010000000
VERSION     DAOPYX010000460.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 1429)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1429)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1429
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_143010_length_1429_cov_8.783843"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..443)
                     /locus_tag="PKN55_14490"
     CDS             complement(<1..443)
                     /locus_tag="PKN55_14490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007343043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HNX72503.1"
                     /translation="MSADFARTPKGRLAGVIFWSRWLQLPIYLGLIVVQGVYAWKFLS
                     QLYKLLANLTSLTEIEIMLTVLGLIDVVMIANLLLMVTVGGYETFVSRLRVEQHPDQP
                     EWLDHVNASVLKVKLSMAIISISSIHLLQTFINAGQLPEHTIKWQL"
     gene            complement(504..1346)
                     /gene="metF"
                     /locus_tag="PKN55_14495"
     CDS             complement(504..1346)
                     /gene="metF"
                     /locus_tag="PKN55_14495"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009116678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HNX72504.1"
                     /translation="MHSQSNPRTFSFEFFPAKTPEGMAKLRTTRQQLAQFKPEFFSVT
                     FGAGGSTRDRTLDTVLEIRNEGYAAAPHLSCIGSSRDNIRAILNEYRNHGIRHIVALR
                     GDIPSGMGEFGEFRFANELVEFIRAEHGDHFHIEVAAYPEYHPQARSAHDDLRNFAVK
                     VKAGANSAITQYFFNADAYFAFVDEVNALGVDIPIVPGIMPINNFSQLARFSDACGAE
                     IPRWLRLKLQSYADDVASIRSLGLDVITALCDRLLAGGAPGLHFYTLNQAGAVSTICQ
                     RLGL"
BASE COUNT          307 a          409 c          452 g          261 t
ORIGIN      
        1 agctgccact tgatggtgtg ctccggcagt tgcccggcgt tgatgaaggt ttgcagcagg
       61 tggatcgacg aaatgctgat gatggccatc gacaacttca ccttgagcac cgaggcgttg
      121 acatggtcca gccattccgg ctgatccgga tgctgttcca cccgcaagcg cgagacaaag
      181 gtttcgtagc cgcccaccgt caccatcagc agcaggttgg cgatcatcac cacgtcaatc
      241 agccccagca cggtgagcat gatctcgatc tcggtcaggc tggtcagatt ggccagcagc
      301 ttgtacagct gggacagaaa cttccaggcg tacacccctt gcacgacaat cagcccgaga
      361 taaatcggca gttgcagcca gcggctccag aaaatcaccc cggccaggcg gcctttcggg
      421 gtgcgggcga aatcggcgct catcgtatcc tccatgatat gggcagccag ctgacccagc
      481 gatgggccag ccggttcccg aatttacagc cccagacgct ggcagatggt ggacaccgcg
      541 ccagcctggt tcagcgtata aaaatgcagg cccggcgcac cgccggccag cagacggtca
      601 cacagggcgg tgatcacatc cagaccgagc gaacggatcg acgccacatc gtcggcgtag
      661 ctttgcagct tcaggcgcag ccagcgcggg atttctgcgc cgcaggcgtc ggaaaagcgc
      721 gccagctggc tgaaattatt gatcggcatg atgccgggca cgatggggat gtccacgccc
      781 agcgcgttga cttcatcgac aaaagcaaaa taggcgtcgg cgttgaagaa atactgggtg
      841 atcgccgagt tggcgccggc tttgaccttg acggcaaaat tgcgcaggtc gtcatgcgcg
      901 ctgcgcgcct gcgggtggta ttccgggtag gcggccactt caatgtgaaa atggtcgccg
      961 tgttcggcgc gaatgaactc gaccagttca ttggcaaagc ggaactcgcc aaactcgccc
     1021 atgccggacg gaatgtcgcc gcgcagggcg acaatatggc gaatgccgtg gttgcggtat
     1081 tcattgagaa tggcgcggat attgtcgcgg ctggagccaa tgcacgacag atgcggcgcg
     1141 gcggcgtagc cttcgttgcg aatttccagc acggtgtcca gcgtgcggtc gcgggtggag
     1201 ccgcccgcgc caaaggtcac cgagaagaac tccggcttga actgcgccag ttgctggcgg
     1261 gtggtgcgca gcttggccat gccttccggg gttttggccg ggaagaactc aaagctgaaa
     1321 gtgcgcggat tcgattgaga gtgcatgaca gacatcctgg gcaagtggcc agcccaaggc
     1381 tggcaacaga aaattttgca acaaaaaggc cggaagcccg gcctttttg
//
LOCUS       DAOPYX010000461         1428 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_143164_length_1428_cov_8.341588, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000461 DAOPYX010000000
VERSION     DAOPYX010000461.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 1428)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1428)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1428
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_143164_length_1428_cov_8.341588"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            51..1271
                     /locus_tag="PKN55_14500"
     CDS             51..1271
                     /locus_tag="PKN55_14500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019923162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNX72505.1"
                     /translation="MFIEFFLALRAAGVPVSLKEFLLLLQALQARLAFASLDDFYYLS
                     RAALVKDEKWFDRFDRVFANHFNGASWDLENLQRDIPEDWLRKLVEKHLSEEDKAALE
                     AMGFKQLMDTLKQRLAEQRERHQGGNKWIGTGGTSPFGAYGYNPAGVRIGQDGSRHRR
                     AVKVWDQREFRDLDGDSELGARGMKIALRRLRQFARDGATEELDIDATIAATARQAGL
                     LDLRYHPRLHDSTKVLLLLDIGGSMDDHVHTVETLFSAVRSEFKHLEHFYFHNCVYES
                     LWKHNARRWNERTPTLDMLHTYGRDYQLILVGDARMSPYELVYPGGSVEHHNEEAGQV
                     WLKRLLAHYPHAVWINPCQEAHWDYSESTRLIHQLMAGRMCPLTVEGLERGIRMLKQS
                     RLGVVPEAQHSGPG"
BASE COUNT          299 a          456 c          429 g          244 t
ORIGIN      
        1 ctctaaaatt gagaataaac gattgtttta accccgacaa gggctgcgcc atgttcatcg
       61 agttttttct tgccctgcgc gccgctggcg tgccggtcag ccttaaggaa tttttgctgc
      121 tcttgcaggc gctgcaagcg cggctggcgt ttgccagtct ggacgacttt tactatctgt
      181 cgcgcgccgc gctggtgaaa gacgaaaaat ggtttgaccg cttcgaccgg gtgttcgcca
      241 accacttcaa cggcgccagc tgggatctgg aaaacctgca acgcgacatc ccggaagact
      301 ggctgcgcaa gctggtggaa aaacacctga gcgaagaaga caaagccgcg ctggaggcca
      361 tgggcttcaa gcaactgatg gacaccctca agcagcggct ggccgaacag cgcgagcgcc
      421 atcagggcgg caataaatgg attggcacag gcggcaccag cccgtttggc gcctatggct
      481 acaacccggc cggggtgcgc atcggccagg atggctcacg ccaccgccgg gcggtcaaag
      541 tgtgggatca gcgcgaattt cgcgatctgg acggcgacag cgagctgggc gcgcgcggca
      601 tgaaaatcgc cctgcgccgg ctgcgccagt ttgcccgcga cggcgccacc gaagaactgg
      661 acatcgacgc caccatcgcc gccaccgccc gccaggccgg ccttttggac ctgcgctacc
      721 accccagact gcacgacagc accaaggtgt tgctgctgct ggacatcggc ggctcgatgg
      781 acgaccacgt ccacacggtg gaaaccctgt tcagcgcggt gcgcagcgaa ttcaaacacc
      841 tggagcactt ttactttcat aactgcgtgt acgaaagcct gtggaagcac aacgcccgcc
      901 gctggaacga acgcaccccc acgctggaca tgctgcacac ctacggccgc gactatcagc
      961 tgattctggt cggcgacgcg cgcatgagcc cgtatgaact ggtctacccc ggcggcagcg
     1021 tggaacacca caacgaagaa gccggccagg tgtggctcaa acgcctgctg gcccactacc
     1081 cgcacgccgt gtggatcaac ccctgccagg aagcgcactg ggactacagc gaatccaccc
     1141 ggctgatcca tcaactgatg gccgggcgca tgtgcccgct gacggtggaa gggctggagc
     1201 gggggatccg gatgttgaag caaagccgcc ttggcgtggt gcccgaagct cagcacagcg
     1261 gtcctggcta ggacgtgtca ccaacaagta cggcggggtc acccaacctc gccaggcgca
     1321 ttttgtgagc cgcgcgaaaa ggaacagcgc agtgcatgcc tgcgtgtgtt tgtcctcgcc
     1381 atcaggccag caacatcacc ttgcccttat tgtgattgcc ctccaccc
//
LOCUS       DAOPYX010000462         1391 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_149200_length_1391_cov_12.087575, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000462 DAOPYX010000000
VERSION     DAOPYX010000462.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 1391)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1391)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1391
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_149200_length_1391_cov_12.087575"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..220)
                     /locus_tag="PKN55_14505"
     CDS             complement(<1..220)
                     /locus_tag="PKN55_14505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX72506.1"
                     /translation="MMGSRDRELAILQSRLFLELRRTHCRVIDLKWMNEDARYARHVL
                     DLAAHCDEDDIRELGRKIAQHMHMPPSAE"
     gene            complement(667..1005)
                     /gene="glnK"
                     /locus_tag="PKN55_14510"
     CDS             complement(667..1005)
                     /gene="glnK"
                     /locus_tag="PKN55_14510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HNX72507.1"
                     /translation="MKLVSAVIKPFKLDEVREALSTIGVQGVTVTEVKGFGRQKGHTE
                     LYRGAEYVVDFLPKVKIDVAIKDELLDQVLEAIEKSAHTGKIGDGKIFVFELEQVVRI
                     RTGETGPEAV"
BASE COUNT          271 a          412 c          411 g          297 t
ORIGIN      
        1 cttcggcgct gggcggcata tgcatatgct gggcaatctt gcggcccagc tcgcggatgt
       61 cgtcttcgtc acagtgcgcg gccaggtcca gcacatggcg ggcgtagcgg gcgtcttcgt
      121 tcatccattt caggtcaatc acccggcagt gggtgcggcg cagctccaga aacagccggc
      181 tttgcaaaat ggccagttcg cggtctcgac ttcccatcat gatgtgtctc cttgcttggg
      241 cagcatggct gaactgtgcc ggaaggcggc aaggacacac gctgcgtgga tgtccatgac
      301 tcagcaggtt tcatgcctgc gagtaaaaat ccagcaaaaa gcggcaaaaa tccataaaaa
      361 tctggctata tccggcaaaa acggattgat tagatggcca tgtgttggtc gcgctggcgc
      421 agagccgcca attgctgggg ccagggtgtc gcgatgtgtg caccacgcca gacgccgcca
      481 atatgcaaca cgccctggcg gcagggttgc cgtcagggcg tgtgtggggg tgactgcgct
      541 aagcgcggct aacaaaactc tcctggcgtt gttgcacgac cttgccgtac tacccgtact
      601 gtctacggtc gtgcgcctag ccaggatcgc tgcgctgggt tttgttgccg cgcttaaaag
      661 gcgctgttac accgcttccg ggccggtttc gccggtgcgg atgcgcacca cttgttccag
      721 ctcgaacaca aaaatcttgc cgtcgccgat tttgccggtg tgggcggatt tctcgatggc
      781 ttccagcacc tggtcgagca gctcatcctt gatggccacg tcgattttca ccttgggcag
      841 aaagtccacc acatattcgg cgccacgata cagctcggtg tggccttttt gacgaccaaa
      901 gcctttgact tcggtgacgg tcaccccttg cacgccgatc gtcgacaggg cttcgcgcac
      961 ctcgtcgagt ttgaacggct tgatgacggc ggataccagt ttcatacaag ctccttgatg
     1021 gttgaatgtt ggatggggcc gcaacgctgc gcccaatggc gtgctggcga gcttgcataa
     1081 ttcgtgccat gccgcaaaac gtccgccgat ggggtttgag cgccagcgcg gcgcagtcat
     1141 gcaccgcaga caggcaaagc cgcgcactgc aatagtgcat gctgtttggc tgaaacctgc
     1201 cgcgcaggca ttcagttgtc agccgcagcg cgccggcttt tgctacactg ctatccagat
     1261 actcatacgg cgtgaacctg cgcgccgcca cggcggtcta tcacgctgcc gcccaaccgc
     1321 atcaaggagt cagccatgct gagccaaaaa ctgttcgacg aaatcagcgc caagattggc
     1381 gacaccctgg c
//
LOCUS       DAOPYX010000463         1378 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_151364_length_1378_cov_14.663643, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000463 DAOPYX010000000
VERSION     DAOPYX010000463.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 1378)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1378)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1378
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_151364_length_1378_cov_14.663643"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            36..>1378
                     /gene="mnmE"
                     /locus_tag="PKN55_14515"
     CDS             36..>1378
                     /gene="mnmE"
                     /locus_tag="PKN55_14515"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003779690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HNX72508.1"
                     /translation="MSAVRLPTIAAIATAPGRGGIGVIRLSGQDLLPMARALSGGKAP
                     KPRVAVFTDFPDADGQPLDNGLLLFFPAPHSFTGEDVIELQGHGGPVVMQRLLRHCLS
                     LGARLAEPGEFTRRAFLNHKLDLAQAESVADLIDAASETAAASALKSLKGEFSQRIHA
                     LVDQLIHLRMLTEATLDFPEEEDVDYLAAADAQGQLRGIQATLDGVLASARQGALLRE
                     GMHVVLVGQPNVGKSSLMNALAGDDIAIVTDIAGTTRDTVREQILIDGIPLHLIDTAG
                     LRDTDDVVEQHGIARTWAAVERADLALVLLDDRAGLTDADAEILARLPARLPRLVVRN
                     KIDLSGQAAGEGEHDGERVLRLSARSGDGVDALRRALLAQIGWQGAGDGVFLARERHL
                     DALRRARAFLDAAELDYAHSELFADHLRMAQLALSDITGEFSADDLLGEIFGRFC"
BASE COUNT          211 a          448 c          478 g          241 t
ORIGIN      
        1 ccccacgcca cttcacccgt tttcaggaat ttgtcatgtc cgctgttcgt ttgcccacca
       61 ttgccgccat tgccaccgcg cccggtcgcg gcgggattgg cgtgattcgt ttgtccggtc
      121 aggatttgct gccgatggcg cgcgcgctca gtggcggcaa ggcgcccaag ccgcgtgtgg
      181 cggtgttcac ggattttccg gatgccgacg gccagccgct ggataacggt ttattgctgt
      241 ttttcccggc gccgcattcg tttaccgggg aagacgtgat cgagctgcaa ggccatggcg
      301 ggccggtggt gatgcagcgc ttgttgcgcc attgcctgag cctgggcgcg cggctggccg
      361 agccggggga gtttacccgg cgggcgtttt tgaatcacaa gctggatttg gcgcaggcgg
      421 agagcgtggc ggatttgatc gacgccgcca gcgaaaccgc cgccgccagc gcgctcaagt
      481 cgctgaaagg ggagttctcc cagcgcattc acgccttggt cgatcagctg attcacctgc
      541 gcatgctcac cgaagccacg ctggatttcc ccgaggaaga agacgtcgat tacctggcgg
      601 cggcggatgc gcagggccag ttgcgcggca ttcaggccac gctggacggg gtgctggcca
      661 gcgcccgcca gggcgctttg ctgcgtgagg gcatgcatgt ggtgctggtc ggccagccca
      721 atgtcggcaa atccagcctg atgaacgcgc tggccggcga cgacatcgcc atcgtcaccg
      781 acatcgccgg caccacccgc gacacggtgc gcgagcaaat cctgatcgac ggcattccgc
      841 tgcacctgat cgacaccgcc ggcctgcgcg acaccgacga tgtggtcgag caacacggca
      901 tcgcccgcac ctgggcggcg gtggagcgcg ccgacctggc cttggtgctg ctggacgacc
      961 gcgccggatt gaccgacgcc gacgccgaga ttctggcccg cctgccggcg cggctgcccc
     1021 ggctggtggt gcgcaataaa atcgacctga gcggccaggc cgccggcgaa ggcgagcacg
     1081 acggcgagcg ggtgctgcgg ctgagcgcgc gcagcggcga cggcgtggac gcgctgcgcc
     1141 gcgcgctgct ggcgcaaatc ggctggcaag gcgcaggaga cggcgtgttt ctggcccgcg
     1201 aacgccacct ggacgccctg cgccgcgccc gcgcctttct ggacgccgcc gagctggact
     1261 acgcgcacag cgaactgttt gccgaccacc tgcgcatggc gcagctggcg ctaagcgaca
     1321 tcaccggcga attcagcgcc gatgatttgc tgggcgagat ttttgggcgg ttttgtat
//
LOCUS       DAOPYX010000464         1355 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_155498_length_1355_cov_3.621538, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000464 DAOPYX010000000
VERSION     DAOPYX010000464.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 1355)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1355)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1355
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_155498_length_1355_cov_3.621538"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(119..>1355)
                     /locus_tag="PKN55_14520"
     CDS             complement(119..>1355)
                     /locus_tag="PKN55_14520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX72509.1"
                     /translation="APMLRWLRRGLRPLPADALPTLGFHLSVHAGEDYAHPASGMRHI
                     DETVQFCEMRDGDRLGHALALGIAPAQWAERQGDMLLPVDEHLDNLVWLWHYACELSS
                     RLPLAAQVLPGLARRVARFVRHLPWLNGETTPLLHAREGAPPASNANLAVEPDTLWRA
                     WRLRRNCYEQLCLAAGLIELEGAVSDSILALATPDWRLLHKASQACALPEKSGHSQPN
                     AALLPATLYLKREAWLALPDSERAPLKKVRVRAGEPLSAAALHHDDPEQGLLQDHDSP
                     AELSFMHALQDYLLDQYDRQGLLIEANPTSNVYIARLQGYHEHPIFRWSPPDESVLQA
                     GGEHNRHGLRRGPMRVLVNTDDPGIMPTTLRTEYALLREAAIDLGISRTVAETWLERL
                     RELGVDQFLRNHQPVFTRR"
BASE COUNT          253 a          446 c          425 g          231 t
ORIGIN      
        1 gccagcttgc ttcaccgccg ttttacccat acgcggatag catcgcgccc aggccagaaa
       61 aaccccgcat ggccattttg cggggtttta tgttttacac gagcctatcc gcacacctct
      121 accgccgggt gaacaccggc tgatgattgc gcagaaactg atccaccccc agctcgcgca
      181 agcgctccag ccaggtttcg gcaacggtgc gggaaatccc cagatcaatc gccgcctcgc
      241 gcaacagcgc atactcggtg cgcagcgtgg tgggcatgat gccggggtcg tcggtgttga
      301 ccagcacccg cattggcccc cggcgcaagc cgtggcgatt gtgctcgcca ccggcctgca
      361 acaccgactc atccggcggc gaccagcgga aaatcgggtg ttcgtggtag ccctgcaagc
      421 gggcgatata cacattggaa gtgggattgg cctcgatcaa cagcccctga cggtcgtatt
      481 gatccagcag ataatcttgc agcgcatgca tgaagctcag ttcggccggg ctgtcgtggt
      541 cttgcagcaa gccttgctcg gggtcgtcat ggtgcagggc ggcggcagac agcggctcgc
      601 cggcgcggac acgaactttt ttcagcggcg cccgctcgct gtcgggcaag gccagccagg
      661 cttcgcgctt cagatacaag gtggcgggca gtaaggcggc gttcggctgg ctgtggccag
      721 acttttcagg caaggcgcaa gcttggcttg ctttgtgcag cagccgccaa tccggcgtgg
      781 ccaacgccaa tatgctgtcg ctgaccgcgc cttccagctc aatcaaacct gccgccaggc
      841 atagctgttc gtagcaattg cgccgcaagc gccaggcgcg ccacagggtg tcgggctcga
      901 cagccaagtt ggcgttgctg gctggcgggg ctccttctcg ggcatgcagc aagggcgtgg
      961 tctcgccatt cagccatggc aagtggcgca caaagcgggc cacccgccgc gccagccccg
     1021 gcaacacctg cgccgccaag ggcaggcggc tgctgagctc gcaagcgtaa tgccacagcc
     1081 acaccagatt atccagatgc tcgtccaccg gcagcagcat atcgccctgc cgctcggccc
     1141 actgcgccgg agcaatgccc aaggccagcg catggcccag ccggtcgcca tcgcgcattt
     1201 cgcaaaactg cacggtttca tcaatatggc gcatgccaga cgccgggtgg gcgtaatcct
     1261 cgccggcatg cacgctcagg tgaaagccca gggtgggtaa ggcgtctgct ggcaaagggc
     1321 gcaagccgcg ccgcagccag cgcaacatgg gcgcg
//
LOCUS       DAOPYX010000465         1337 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_158756_length_1337_cov_12.655226, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000465 DAOPYX010000000
VERSION     DAOPYX010000465.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 1337)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1337)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1337
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_158756_length_1337_cov_12.655226"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(19..447)
                     /locus_tag="PKN55_14525"
     CDS             complement(19..447)
                     /locus_tag="PKN55_14525"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HNX72510.1"
                     /translation="MSALRPDISVAVLNQRGQEYLPGYLGIEILSLAPGQLNSRMPIR
                     KLHIAPNEFLHAASVVALADTSCGYATIAHLPPGAQSFTTIELKSNHLGTVTEGAVAC
                     VANAQHLGRSTQVWDAVVSDEASGRTLALFRCTQMILWPK"
     gene            559..>1337
                     /locus_tag="PKN55_14530"
     CDS             559..>1337
                     /locus_tag="PKN55_14530"
                     /inference="COORDINATES: protein motif:HMM:NF014068.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HNX72511.1"
                     /translation="MTDIAFLLPPGFFTSGVTGLIDAFMLANRQMATRGGPGLRWRLL
                     SADGGPVAASSGLRLAADGSFAELRAGEVIIVPGVMFDDVASFERVLASHADLVARLR
                     GWREAGHVLAAHCTGVALLAEAGVLDGQPATLSWWLAGWFRRRYPAVQLDAQALVTES
                     AHILCSGATSAYQDLALKLIARLLGEDVAQVCARILLIDPNRVSQAPYATLAAYNGHN
                     DPLVSDCQRWMHARLDQPFSLAALAAAVHSSERTLMRRFRQA"
BASE COUNT          186 a          426 c          476 g          249 t
ORIGIN      
        1 gcttgcgcgg ccacgcgctt acttcggcca caaaatcatc tgcgtgcagc gaaacagcgc
       61 cagcgtgcgc ccgctggcct cgtcgctgac cacggcgtcc cacacttgcg tgctgcgccc
      121 caagtgctgg gcgttggcca cgcaggccac agcgccttcg gtgacagtgc ccaggtgatt
      181 gcttttcagc tcaatggtgg tgaacgactg cgcgcctggc ggcaggtggg cgatggtggc
      241 gtagccgcag ctggtgtcgg ccagcgccac cacgctggcg gcgtgcagaa actcattggg
      301 ggcgatgtgc agtttgcgga tgggcatgcg gctgttcagc tggccagggg ccaggctgag
      361 gatttcaatg cccagataac cgggcagata ttcctggccg cgctggttca gcacggccac
      421 gctgatgtcg gggcgtaggg cggacacggt gtgctcctgt gtgagcggtc gggatgaccg
      481 gcttgcccac agtctgcgct tgccgtgcgc gggcgcgtag tggcaggatt gccatttttc
      541 aggtcaatcc cgccaaccat gaccgatatt gcctttttgc tgccgccagg gtttttcacc
      601 agcggcgtca ccggattgat cgacgccttt atgctggcca accgccagat ggcgacgcgc
      661 ggcgggccag ggctgcgctg gcgcttgctc agcgccgacg gcggcccggt ggcggcgtct
      721 tctggcttgc gcctggcggc ggatggcagc tttgccgagc tgcgcgctgg cgaggtgatt
      781 attgtgccgg gggtgatgtt tgacgatgtc gccagcttcg agcgggtgct ggccagccat
      841 gccgacttgg tggcgcgcct gcgcggctgg cgcgaggccg gccatgtgct ggcggcgcat
      901 tgcactggcg tggcgctgct ggccgaggcc ggcgtgctgg atggccagcc ggccacgctc
      961 agctggtggc tggccggctg gtttcgccgc cgctacccgg ccgtgcagct ggatgcgcag
     1021 gcgctggtca ccgaaagcgc gcacatcctg tgcagcggcg ccaccagcgc ctatcaggac
     1081 ttggcgctga aactgattgc ccggctgttg ggcgaggatg tggcgcaagt ctgcgcgcgg
     1141 attttgctga tcgaccccaa ccgcgtgtca caagcgccct acgccaccct ggccgcctac
     1201 aacggccata acgacccctt ggtcagcgac tgccagcgct ggatgcatgc ccggctggat
     1261 caacccttca gcctggccgc gctggcggcg gcggtgcaca gcagcgagcg cacgctgatg
     1321 cgccgctttc ggcaagc
//
LOCUS       DAOPYX010000466         1302 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_165480_length_1302_cov_7.253408, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000466 DAOPYX010000000
VERSION     DAOPYX010000466.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 1302)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1302)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1302
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_165480_length_1302_cov_7.253408"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..899)
                     /locus_tag="PKN55_14535"
     CDS             complement(<1..899)
                     /locus_tag="PKN55_14535"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HNX72512.1"
                     /translation="MIYRREIDGLRALAVLPVMLFHAGFSGFSGGFVGVDVFFVISGY
                     LITGILINEQANGTFTLTGFYERRARRILPALFVVLLACLPFAWAWLLPSDLKAFARS
                     LAAAAISLSNILFWRESGYFDTSSELKPLLHTWSLGVEEQYYMVFPLLLLGLARWRPH
                     WTPHVLWAGAALSLALAQWAALHQPSLAFYLLPTRGWELLAGALLAYYHSRPATRIPH
                     AAALNQAGSLLGLAMVAYAVLRFDQHTPFPGVYALLPTLGAVLMIACAQPGTWVSSLL
                     ATPLLVGIGLISYSAYLWHQPVLA"
     gene            complement(1067..>1302)
                     /locus_tag="PKN55_14540"
     CDS             complement(1067..>1302)
                     /locus_tag="PKN55_14540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012698482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HNX72513.1"
                     /translation="VAAMEAEEQRQRPMQFQHAEFNPALADGDGAAELSSDDDSNPFA
                     PEALAAQGLRVGRNDPCPCGSGKKYKQCHGKLG"
BASE COUNT          255 a          410 c          433 g          204 t
ORIGIN      
        1 gccagcaccg gctggtgcca gaggtaggcg ctgtagctga tcaggccgat gcccaccagc
       61 aagggcgtgg ccagcaggct gctgacccag gtgcccggct gggcgcaggc aatcatcagc
      121 accgcgccca gcgtgggcag tagcgcgtac acgccaggaa acggcgtgtg ctgatcaaag
      181 cgcagcacgg cataggccac catggccagc cccaacaggc tgccggcctg attcagtgcg
      241 gcggcatgcg ggatgcgggt ggccgggcgg ctgtggtaat acgccagcaa ggcaccggcc
      301 agcagttccc agcctcgggt gggcagcaga taaaacgcca gcgagggctg gtgtagagct
      361 gcccattgcg ccagcgccag gctcagcgcc gcaccggccc agagcacatg cggcgtccag
      421 tgtggccgcc agcgtgccag acccagcagc agcaggggaa aaaccatgta gtactgttct
      481 tctactccca gactccaggt gtgcagcagc ggcttgagtt ccgaactggt atcaaaatag
      541 ccgctctcac gccagaacag gatattcgac agcgaaatgg cagcggcggc caggctgcgg
      601 gcaaaggctt tcaggtcgct gggcagcagc caggcccagg caaatggcag gcaggccagc
      661 agcaccacaa atagcgcggg caagatccgc cgggcccggc gttcgtaaaa cccggtcagg
      721 gtgaatgtgc cgttggcctg ttcgttgatc agaatgccgg taatcagata gccactgatc
      781 acaaaaaaca catccacacc cacaaagccg ccgctaaacc cgctaaagcc ggcgtggaac
      841 aacatcacgg gaagcaccgc gagggcgcgc aggccgtcaa tttcgcgtcg ataaatcatg
      901 gtcgttgctc ggagagtgaa tcagtggcgg gcgcatgggg cggatcgctc tgacagactg
      961 acaacatgac gccaccttga cctgcaaatt ctggctgcgg gtcaaggtgg cgtcatcggg
     1021 gccgggaacc gcctgtcgcg gcgctcccgg tgtgctggcc ggaggattac cccagcttgc
     1081 cgtggcactg cttgtacttc ttgcccgacc cgcaggggca tgggtcgttg cggcccacgc
     1141 gcaggccctg ggcggccagc gcttctggcg caaacgggtt gctgtcgtcg tcgctgctga
     1201 gttctgccgc gccgtcgccg tcggccagcg ccgggttgaa ctcggcgtgc tggaactgca
     1261 tcggccgctg gcgctgctct tcggcttcca tggcggccac tt
//
LOCUS       DAOPYX010000467         1250 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_176356_length_1250_cov_13.393305, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000467 DAOPYX010000000
VERSION     DAOPYX010000467.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 1250)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1250)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1250
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_176356_length_1250_cov_13.393305"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1250
                     /locus_tag="PKN55_14545"
     CDS             <1..>1250
                     /locus_tag="PKN55_14545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNX72514.1"
                     /translation="GLALVISWFVAVLFTPFIGHWLLPEHQPNTEHGAHGDVYDKPFY
                     VRFRRLVEWCLDHRKTVLAATLTAFVLSLVCFKLFVQQQFFPASSRVELLVDFWLPQS
                     ASYQATETEVKRLETLLKNDERIVSITSYVGGGSPRFYLPLDQQQQHLNYAQMMIMTR
                     DEQVRDEVKHKIAELFDQGFPAVRGRVIRLENGPPVGYPVQFRLLGPDPGQLRAIGQR
                     MEAMLREHPATRQVNNDWGEQLRVMRVTVDQDKARALGVSSQSLARQLQMLLSGSVVT
                     EYRDADRTLEVVARLGADERASLERLPNLMIQTASGRFVPVAQLARVEYKPEESIVWR
                     RNRLPTLTVRADVADGVQAADVSIALWPKMQALEKTLPPGYHIELGGSLESSKKSQDA
                     IGAVQPLMLVVVISLLMFQLHSFQR"
BASE COUNT          214 a          397 c          417 g          222 t
ORIGIN      
        1 ggcctggcgc tggtgatttc ctggtttgtc gccgtgctgt tcaccccgtt catcggccac
       61 tggctgctgc ctgagcacca gcccaacacc gagcacggcg cgcatggcga tgtgtacgac
      121 aagccgtttt atgtgcgctt tcgccggctg gtggaatggt gcctggacca ccgcaagacc
      181 gtgctggccg ccacgctgac cgcgtttgtg ctgtcgctgg tgtgcttcaa gctgtttgtg
      241 cagcagcagt tcttcccggc ctccagccgg gtggagctgt tggtggattt ctggctgccg
      301 caaagcgcca gttatcaggc caccgaaacc gaggtcaaac ggctggaaac cctgctgaag
      361 aacgacgagc ggattgtctc catcaccagc tatgtcggcg gcggctcgcc gcgcttttac
      421 ctgccgctgg accagcagca acagcacctg aactacgcgc agatgatgat catgacccgc
      481 gacgagcagg tgcgcgacga ggtgaaacac aaaatcgccg agctgttcga ccagggcttt
      541 ccggcggtgc gcggccgggt gatccgcctg gaaaacggcc cgccggtggg ctatccggtg
      601 cagttccgtc tgctgggccc cgaccctggc cagctgcgcg ccatcggcca gcgcatggaa
      661 gccatgctgc gtgaacaccc ggccacccgc caggtgaaca acgactgggg cgagcaattg
      721 cgggtgatgc gcgtcacggt ggatcaagac aaggcccgcg cgctgggcgt gtccagccag
      781 tcgctggcgc gtcagctgca aatgctgctg tccgggtcgg tggtcaccga ataccgcgac
      841 gccgatcgca cgctggaagt ggtggcccgg ctgggcgccg acgagcgcgc cagcctggag
      901 cggctgccca atctgatgat ccagaccgcg tccgggcgct ttgtgccggt ggcgcagctg
      961 gcgcgggtgg aatacaagcc cgaagaaagc atcgtctggc ggcgcaaccg cctgcccacg
     1021 ctgaccgtgc gcgccgatgt ggccgacggc gtgcaggcgg ccgatgtgtc gattgccctg
     1081 tggcccaaga tgcaggcgct ggaaaaaacc ctgccgccgg gctatcacat cgagctgggc
     1141 ggctcgctgg aatccagcaa aaaatcgcag gacgccatcg gcgccgtgca acccttgatg
     1201 ctggtggtgg tgatttcgct gctgatgttc cagctgcaca gcttccagcg
//
LOCUS       DAOPYX010000468         1243 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_177876_length_1243_cov_11.458754, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000468 DAOPYX010000000
VERSION     DAOPYX010000468.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 1243)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1243)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1243
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_177876_length_1243_cov_11.458754"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            120..518
                     /locus_tag="PKN55_14550"
     CDS             120..518
                     /locus_tag="PKN55_14550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX72515.1"
                     /translation="MLLGVWADQAGVFLAWLCAITTVVFAVPITFFPLRWARLMRWRI
                     PAETQLTVYFGRCLGLFILILEGLMARAAWSGEGRVWVFEQLAAVFACMVALHVYGAL
                     RREQPWTETAEIGVYSVCLLLTLACFPLPA"
     gene            complement(629..>1243)
                     /locus_tag="PKN55_14555"
     CDS             complement(629..>1243)
                     /locus_tag="PKN55_14555"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNX72516.1"
                     /translation="DHAVVRTGYRRLIDAEADMRVVAEAATADEACAALRRQPVNVAV
                     VDLSLQLGSGMEAISRLLARQPDLAILVFTMHQHAGYARQAFRAGAQGYLTKHADPDD
                     MLAALRKVAQGRRVLSPDMAELLANDSLEADAAISRLTPREFDILRLAVAGEPPARIA
                     EQLHLSAKTVLNYLSAIRQKLEVHNDIGLMQLALRHGLVAAPGA"
BASE COUNT          180 a          381 c          432 g          250 t
ORIGIN      
        1 aagcatcatg gatgaaatgc tggcgagaat ggcgccgatt ctcgccccac ctaagtcgca
       61 taccccgcac catcccgccc gcctagaatc cccgctcatt tattgacgag gtcatcttca
      121 tgttgctagg cgtttgggcc gatcaggcgg gggtgtttct ggcgtggctg tgcgccatca
      181 ccacggtggt gtttgccgtg ccgattacgt tttttccgtt gcgctgggcg cgcttgatgc
      241 gttggcggat tccggcggag acgcagttga cggtgtattt tggccgctgt ctggggttgt
      301 tcattctgat tctggaaggc ttgatggcgc gggcggcgtg gagcggcgag gggcgggtgt
      361 gggtgttcga gcagttggcg gcggtgtttg cctgcatggt ggcgttacat gtgtatggcg
      421 cgctgcgccg tgagcagccg tggacggaga cggcagagat tggggtgtac agcgtgtgct
      481 tgttgttgac gctggcgtgc tttccattgc cggcatagtg ccgccgcaac ctgcccatgg
      541 cgccgtcgca gaaagccact gacagcaagc gggccgtggc cgtgggtggc cacgccaagc
      601 tcattttgat ggtggcgcca accttccttc aggcgcccgg cgcggccacc aagccatggc
      661 gcagcgccag ctgcatcagg ccaatatcgt tgtgcacttc cagcttctgg cgaattgccg
      721 acagataatt gagcacggtc ttggcgctca ggtgcagctg ctcggcaatg cgcgccggcg
      781 gctcgccggc caccgccagg cgcaggatgt cgaattcgcg cggcgtcagc cggctgatgg
      841 cggcgtcggc ttccaggctg tcgttggcca gcagctcggc catgtccggc gacaacaccc
      901 gtcgcccctg cgccaccttg cgcagcgccg ccagcatgtc atccgggtcg gcgtgtttgg
      961 tcagatagcc ttgcgcgcca gcgcgaaacg cctggcgggc gtagccggcg tgctggtgca
     1021 tggtgaacac caggatggcc agatccggct gtcgggccag caggcggctg atggcctcca
     1081 tgccgctgcc cagttgcagc gacaaatcca ccaccgccac gttcaccggc tggcggcgca
     1141 gcgcggcgca ggcttcgtct gcggtggcgg cctcggccac cacgcgcata tcggcctcgg
     1201 cgtcgatcaa gcggcggtag ccggtgcgca ccacagcgtg gtc
//
LOCUS       DAOPYX010000469         1238 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_178991_length_1238_cov_11.021978, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000469 DAOPYX010000000
VERSION     DAOPYX010000469.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 1238)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1238)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1238
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_178991_length_1238_cov_11.021978"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(12..950)
                     /gene="thrB"
                     /locus_tag="PKN55_14560"
     CDS             complement(12..950)
                     /gene="thrB"
                     /locus_tag="PKN55_14560"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008952205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HNX72517.1"
                     /translation="MSVYTTVTQAELESWLAQYTLGQLVELKGIAAGITNTNYFVTTT
                     QGRYVLTLFETLKLEELPYYLNLMSHLARHGVACPAPMADRSDRFVSLLAGKPACLVS
                     CLSGADVAAPNPAQCRAVGVMMADMHLAGDTYPARMANPRGPAWWSATAHVVYPHMDA
                     ADAAMLHEEVAFQSEHRFDALPTGVIHADLFRDNVLFDGDSVAGFIDFYYACNDVLAY
                     DLAIALNDWCALPDGDIDGERARAMLAGYQSVRPLSDAEKAAWPVLLRAAGLRFWTSR
                     LYDKYLPQAGELTFTKDPAQFQRVVALHRQRQDFWL"
BASE COUNT          214 a          378 c          428 g          218 t
ORIGIN      
        1 gtgagccgcg cttacagcca gaaatcctgg cgctggcggt gcagggcaac cacccgctgg
       61 aactgcgccg ggtctttggt gaaggtcagc tcaccggctt gcggcaggta tttgtcgtac
      121 aggcgcgagg tccagaagcg caggccggcg gcacgcagca gcaccggcca ggcggctttt
      181 tcggcgtcgc tgagcgggcg caccgactga tagccggcca gcatggcgcg cgcgcgttcg
      241 ccgtcgatgt cgccatccgg cagcgcgcac cagtcgttca gggcaatggc caggtcgtag
      301 gccagcacat cgttgcaggc atagtaaaaa tcgataaagc ccgccacgct gtcgccgtca
      361 aacagcacat tgtcgcggaa caaatcggcg tgaatcaccc cggtgggcag cgcatcaaag
      421 cggtgctcgc tctggaaggc cacttcttcg tgcagcatgg cggcgtcggc ggcgtccata
      481 tgcgggtaga ccacgtgcgc ggtggcgctc caccaggccg ggccgcgcgg gttggccatg
      541 cgcgccgggt aggtgtcgcc ggccagatgc atatcggcca tcatcacccc caccgcgcgg
      601 cattgcgccg ggttgggcgc ggccacgtcg gcgccgctca ggcaagacac caggcaggcc
      661 ggcttgccgg ccagcaggct gacaaagcgg tcgctgcggt cggccatcgg cgccgggcag
      721 gccacgccgt ggcgcgccag gtggctcatc aggttcagat agtagggcag ctcttccagc
      781 ttgagcgttt caaacagggt cagcacatag cggccctgcg tggtggtgac aaagtaattg
      841 gtgttggtga tgccggcagc gatgcctttc agttcgacca actggcctag ggtgtattgc
      901 gccagccagc tttccagctc ggcctgggtg acggtggtgt aaaccgacat gggatgtctc
      961 ttgagggttc cgggatgggg ggcgtcacgg cctggccgtg acggcgcgtt ttttgccttg
     1021 gggcgtgtgc tgagcgccac tcacaaaacc cgcctggcgt ggttgcccga cgcggtcgtg
     1081 cgcctggcca ggtgcgctgc gctggctagg cgtgtgaccg cagacagtac aagcagcagt
     1141 acggcaaggc cacacaacaa cgccaggggt tttgtgagcg gctcttaagg gatgcgctga
     1201 ttgattcaac acgatcaaca tcctgaattc ccgcctgt
//
LOCUS       DAOPYX010000470         1216 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_184286_length_1216_cov_3.386736, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000470 DAOPYX010000000
VERSION     DAOPYX010000470.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 1216)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1216)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1216
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_184286_length_1216_cov_3.386736"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..571)
                     /locus_tag="PKN55_14565"
     CDS             complement(<1..571)
                     /locus_tag="PKN55_14565"
                     /inference="COORDINATES: protein motif:HMM:NF025611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4347 domain-containing protein"
                     /protein_id="HNX72518.1"
                     /translation="MNPAFFVRHPDTPTLYAAAKPASLIVVDQGIQDWPYLVSLLPAS
                     SAHTEVVILDGGLDQLATHLAHRPAGSLHALHLLCHGRSGGLLLGKQWLTQANLATHA
                     DSLRQIGRALATDAGWWVYGCEVAQGARGQRFVAELAQWSGAQLAAASTPIGAGHGWT
                     LDVQVGQPSRQRIPAWLTKVASQYRGVLAA"
BASE COUNT          267 a          355 c          346 g          248 t
ORIGIN      
        1 aggccgccag cacgccgcga tattggctgg ccaccttggt caaccaggcc ggaatccgct
       61 ggcggctggg ctgaccgacc tgcacatcca gcgtccagcc atgacccgcg ccaattggcg
      121 tggatgccgc ggcaagctgg gcgccgctcc actgtgccag ctcagcgaca aaacgctggc
      181 cacgtgctcc ctgtgccact tcacagccat acacccacca gccggcgtct gtcgccagcg
      241 cgcggccaat ctggcgcagg ctatccgcgt gtgtggccag attagcctgc gtcagccact
      301 gcttgcccag taacaagcca ccgctgcggc cgtgacagag cagatgcagt gcgtgcagcg
      361 aacctgccgg cctgtgcgcc aggtgggtgg ccagttgatc cagcccgcca tccagtatca
      421 ctacctcagt atgtgcagat gaggctggca acaggctaac cagatatggc caatcctgga
      481 tgccctgatc aaccacaatc aatgatgcgg gcttggctgc tgcgtacagg gtgggggtgt
      541 ctggatggcg aacgaaaaaa gcaggattca tgggtctggc gcggtaaaaa tggccgctga
      601 tcgtgacatc agcgccgatc acttcaatcg gtgaaaacgc aatattcatc aaaaaacata
      661 tacggcatgt tttgggcttg aatttagctg gattcgtgtg cgcagggcac cggcaacatg
      721 ctgtttgatt acgtcatgat gcgggccact tcatccccag gtgggtgaaa aaatggccaa
      781 ggcgatgttc agcgccgctc acaaaatccc ttgtggcgtg gcgtatgctc ttacggccac
      841 atgcctaagc acgatcactt cgagttttgt gagcggcgct taaccgggca agattttgct
      901 gcctccgcca tggcgaaacc ttgcacgttt ttcaacgttt gccgatgttg gatagcgggc
      961 caggcaaagt caattgccac cagaacgatt aagacagatt gagcggactt tgcgatgaaa
     1021 tgccggtttc ccagcgcaaa aactccgctc gcaatacgaa taaggccgct caaaaatgcc
     1081 gtgaggcagg cggccgcagg cagtagaact gccgtgcaac aatcccactc gtgttttgtg
     1141 cgtgcttatt aaccgtgttg atctgccgtg atttcatgca tccagcgggt aatgccctgc
     1201 ccgtcatcca gcgcag
//
LOCUS       DAOPYX010000471         1188 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_191311_length_1188_cov_1.938217, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000471 DAOPYX010000000
VERSION     DAOPYX010000471.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 1188)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1188)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1188
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_191311_length_1188_cov_1.938217"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            88..378
                     /locus_tag="PKN55_14570"
     CDS             88..378
                     /locus_tag="PKN55_14570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX72519.1"
                     /translation="MSVCPKMRWAALCLLMTTSAAWAAAPQVRAERLEQRSQQLEQRA
                     DAQQARADRIDVAAASHPKRAERHERRLERRAERADRRAEGLTHRADVLNAR"
     gene            complement(792..>1188)
                     /locus_tag="PKN55_14575"
     CDS             complement(792..>1188)
                     /locus_tag="PKN55_14575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX72520.1"
                     /translation="VDVSRCPDCNKLAPGVGGDKTCAKHAPLIDAGIPPNLRHFAAQK
                     SRTARAWACCAARFNKNQAANAAILPAGHTSPAGAPKAFRWDVGHGENGDAGQARRRT
                     SRAGVATAAQRGAAACIHTLAAGNTPAAA"
BASE COUNT          194 a          404 c          361 g          229 t
ORIGIN      
        1 agccgccaag caataacctc gcctcgccca cgcgcgtcgg actgcctcag cccgacgcgc
       61 ggcgctgaat cctgactgga gaccgccatg tctgtttgcc cgaaaatgcg ctgggccgcc
      121 ttgtgcctgc tgatgaccac ttcggccgcc tgggctgctg cgccgcaagt tcgcgctgag
      181 cgtctggagc agcgcagcca gcaactggaa caacgcgccg acgcccaaca ggctcgcgcc
      241 gaccgcattg atgtcgcggc cgccagtcac cccaaacgcg ccgaacgcca tgaacgccgc
      301 ctggagcgcc gcgcagagcg tgctgaccgg cgagccgaag ggctgacaca tcgggcggat
      361 gtgctgaacg cacgctgagt ctgatcctgt tttgctgcgc gccgacggcc tgacaccctg
      421 tcggcgcgca ttcctgtatg ctgttccgca ctactcatcc cacgcttttg gcgtgattgc
      481 gctgcctgcc tgcatcgctt cgccgcgtgt tgtgagaggc aatcgtcagc cagcaacttg
      541 ctggcgatat tgccgtcaaa caagctgaat cgcgtcactt gcgctgttcc tctcgccagg
      601 atgacggcgc tggctggtgt gagcgaggct taatttatcc acgacgggca tgactgcctt
      661 tttgcggagc ctgatcatga aactgggcgt gtctcttctt ttggtgtggc ttaccgctca
      721 ggccacggct tgggcgcagc cggcgtatcc tcgacaaact tatccacagg ggccgtcggt
      781 gcagatcacc gtcacgccgc cgccggtgta ttacccgccg ccagcgtatg gatacacgcc
      841 gccgcgccgc gctgagccgc agtggcaacg cccgcccggc tggtacgacg acgcgcctgg
      901 ccagcgtcgc cattctcacc gtggccaaca tcccaacgaa acgccttggg agcgcccgcc
      961 gggctggtat gacccgccgg caggatcgcg gcgttagccg cctggttttt gttgaatctt
     1021 gccgcacagc aggcccacgc ccttgctgtg cggctttttt gcgcggcgaa atgtcgcaga
     1081 ttgggcggga tgccggcgtc aatcagcggc gcatgctttg cacatgtctt atcgcccccc
     1141 acgccgggcg cgagcttgtt acaatcaggg catcgtgaga catccacg
//
LOCUS       DAOPYX010000472         1186 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_191659_length_1186_cov_7.817860, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000472 DAOPYX010000000
VERSION     DAOPYX010000472.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 1186)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1186)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1186
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_191659_length_1186_cov_7.817860"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..215)
                     /locus_tag="PKN55_14580"
     CDS             complement(<1..215)
                     /locus_tag="PKN55_14580"
                     /inference="COORDINATES: protein motif:HMM:NF025149.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4172 domain-containing protein"
                     /protein_id="HNX72521.1"
                     /translation="MYTDTPLYIWQAADWPHWRYDLNALAAPLAEVSHAQGVLLGRLA
                     DVGMDWRERASLAALTDEVLHTSAIEG"
     gene            539..1156
                     /locus_tag="PKN55_14585"
     CDS             539..1156
                     /locus_tag="PKN55_14585"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HNX72522.1"
                     /translation="MPIAQECPQDLAPYLAASSIINWTHPAVLAQARALAAGLTDEEA
                     IARACFEFVRDDIRHSLDAQANPVTCTASEVLAHRIGYCYGKSHLLAALLRANGLPTA
                     LCYQRLSVDDNGPPYCLHGLNAVYLARHGWYRLDARGNKPGVAAEFCPPREALAFALR
                     LPGEVDVPGRFAEPLAAVVQALTTHDDYRAVAAQLPDWPLADLPA"
BASE COUNT          183 a          352 c          406 g          245 t
ORIGIN      
        1 tcgccttcaa tggcgctggt gtgcagcact tcgtcggtca gcgccgccag gctggcgcgc
       61 tcgcgccaat ccatgcccac atcggccagc cggcccagca acacgccttg cgcatggctg
      121 acttcggcca acggcgccgc caaggcgttc aggtcgtagc gccaatgcgg ccagtcggcg
      181 gcttgccaga tataaagcgg ggtgtctgtg tacatccgcc gagtatgccc gcgctgcggc
      241 gaatgcgtcc atattctccg cacaatctgc ggtgattaag cggcgattga cgcgcgctcg
      301 cgtctgctgg atgataaagg catgaatcgc tctacgtctt tcctgtgccg atgcccttgg
      361 ctctcccgac cgtgttcggg tgggccgggc tgtgtgcgtg tgcggcaggg ggatgtgcgc
      421 ctttgcaggg ggcgaagtta gcgttttctc gttgcagttt tgcgcaaggc cacggcaaat
      481 gtcgtggcct ttttatttgc cgttcaggca tgtgatggaa tctgtgaaag gaagggcaat
      541 gcccatcgct caagaatgcc cgcaggattt ggcgccgtat ctggccgcca gctcgatcat
      601 caattggacg cacccggcgg tgctggcgca ggcgcgcgca ctggcggcgg gcctgacgga
      661 tgaggaggcg attgcccgcg cctgttttga gtttgtgcgc gacgatattc gccatagttt
      721 ggatgcgcag gccaacccgg taacctgcac ggcgtcagag gtgctggcgc accggattgg
      781 ctattgctac ggcaaaagcc atttgctggc ggcgctgctg cgcgccaatg gtctgcccac
      841 cgccttgtgt tatcagcggc tgagcgtgga tgacaatggc ccgccgtatt gcctgcatgg
      901 tttgaatgcg gtgtatctgg cccgccatgg ctggtatcgg ctggatgcgc gcggcaacaa
      961 gccaggggtg gcggccgaat tttgcccgcc gcgcgaggca ttggcgtttg ccctgcgtct
     1021 gcctggcgag gtggatgtgc cggggcgctt tgccgagccg ctggccgccg tggtgcaggc
     1081 gctcaccacg catgacgatt atcgggcggt ggcggcacag ctgccggact ggccactggc
     1141 cgatctgccc gcataaaaaa cggcgcccgg gggcgggcgc cgcaat
//
LOCUS       DAOPYX010000473         1180 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_193447_length_1180_cov_1.412444, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000473 DAOPYX010000000
VERSION     DAOPYX010000473.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 1180)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1180)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1180
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_193447_length_1180_cov_1.412444"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(124..>1180)
                     /locus_tag="PKN55_14590"
     CDS             complement(124..>1180)
                     /locus_tag="PKN55_14590"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNX72523.1"
                     /translation="LLLLLCFVWLWPGILGHEPWKPDEPYALGVVQWMLHSGDWQVPR
                     IVGEAYLDTPPLYYWVAAGFARLLSPWLALHDAARLATPLFMALTLLFTGMTARELIG
                     RRHGRSAVLILIGCLGLIESGHQLSPDVAMLCAESACLLGLALAQRRGFLAALALALG
                     STALLLSASLMEVAVVWLAVLLLLLLPGWRSRQYIKTVTISLALGMPLALLWPLSMLK
                     HEPALFAQWWYSLALGPFYGFGRLTLLHEFGYYLRLLPWFAWPAWPLAAWAVWRGRER
                     LNAARYQLPLLLIAINLLVLWLSPRQNALYALPLLLPMAVLAAVELDTLKRGAAAFFN
                     WFGVLTLGLAGGLRAHA"
BASE COUNT          266 a          414 c          372 g          128 t
ORIGIN      
        1 cgcggcgacg ctgccgcggc ggctggcgcg cgagacgatg caggcgctga cggtgcatcc
       61 ggacgccaag gtccgcgcga tgacgaacgc ggtcctcgcg gggatgcgcg agccggcgga
      121 gggtcaggcg tgagcccgca agccgccagc caggcccagg gtgagcacgc caaaccagtt
      181 gaaaaacgcg gcagccccgc gcttgagggt atcgagctcc acggcggcca gcaccgccat
      241 cggcagcaac agcggcaagg catacagggc gttctggcgt ggcgacagcc acagcaccag
      301 taaattaatt gctatcagca gcagcggcag ctgatagcgt gcggcattca agcgttcgcg
      361 cccccgccac accgcccagg ccgccagcgg ccacgccggc caggcaaacc acggcaataa
      421 acgcaggtaa tagccaaact cgtgcagcag tgtcagccgg ccaaaaccat aaaacggccc
      481 cagcgccagc gaataccacc attgggcaaa cagcgccggc tcgtgcttga gcatcgacag
      541 cggccacaac agcgccagcg gcatgcccag cgccaatgag atggtcactg tcttgatgta
      601 ctggcggctg cgccagccag gcagcagcaa gagcagcaac accgccagcc acaccacggc
      661 cacttccatc aggctggccg acaacaacag cgcggtgctg cccagcgcca gcgccagcgc
      721 cgccagaaac ccccggcgct gcgccagggc cagccccagc aagcaggccg attcggcgca
      781 gagcatggcc acatcgggcg acagctggtg gcccgactca atcaggccca ggcagccaat
      841 caagatcagc acggcactgc ggccatggcg gcggccaatc agctcgcggg cggtcatgcc
      901 ggtaaacagc agggtcagcg ccataaataa aggcgtggcc agccgggcgg cgtcgtgcag
      961 cgccagccag ggtgacaata accgggcaaa cccggcggcc acccagtaat acagcggtgg
     1021 cgtatccaga taggcttcgc cgacaatgcg cggcacttgc cagtcgccgc tgtgcagcat
     1081 ccactgcacc acccccagtg cgtatggctc gtcgggcttc cacggttcat ggccgagaat
     1141 gcccggccac agccagacaa agcacagcag caacagcagc
//
LOCUS       DAOPYX010000474         1165 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_197156_length_1165_cov_4.325225, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000474 DAOPYX010000000
VERSION     DAOPYX010000474.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 1165)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1165)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1165
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_197156_length_1165_cov_4.325225"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..243
                     /locus_tag="PKN55_14595"
     CDS             <1..243
                     /locus_tag="PKN55_14595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018152273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HNX72524.1"
                     /translation="LPLVRFGMLASWVLIFMIFEREYSTGVYLLTPGTEVIGALLVSL
                     WAAGSIDLVAALSVINISLVAAGLAIALRFGVKLHD"
     gene            236..>1165
                     /locus_tag="PKN55_14600"
     CDS             236..>1165
                     /locus_tag="PKN55_14600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018748334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNX72525.1"
                     /translation="MTKQNNTLKVDSLCLQYGDNAILKGVSFELKPGEVVSLLGASGS
                     GKTTLLRAVAGLEHPSSGRIVIGDSVLFDGAARLDLPVEKRGLGLVFQSYALWPHRTV
                     FDNVAYGLKLRKVADADIRQRVDQALASLDLGHLAARFPHQLSGGQQQRVAIARALVY
                     NPPVILLDEPLSNLDAKLREEARSWLRELIVTLGLSALCVTHDQTEAMAMSDRILLLK
                     DGRIEQQGSPQDMYDNPASLFVADFMGNNNRLNGQISEVRDGRAKISGEGWSFWGSLR
                     GDAQPGRAATAMIRLERVRVADGPGDNHIALPLV"
BASE COUNT          189 a          383 c          382 g          211 t
ORIGIN      
        1 ctgccgctgg tgcgctttgg catgctggcc agctgggtgc tgattttcat gattttcgag
       61 cgggaatact ccaccggggt gtacctgctg accccgggca ccgaagtgat tggcgccttg
      121 ctggtgtcgc tgtgggccgc cggctcgatc gatctggtcg ccgcgctgtc cgtgatcaat
      181 atctcgctgg tggccgccgg tctggcgatt gccctgcgct ttggggtgaa actccatgac
      241 taagcaaaac aataccctga aggtggattc gctctgcctg caatacggtg acaacgccat
      301 cctcaagggc gtgtcgtttg aactcaaacc cggcgaagtg gtgtcgctgc tgggcgcctc
      361 cggcagcggc aaaaccaccc tgctgcgcgc ggtggccggc ctggaacacc cgtccagcgg
      421 tcggattgtg attggcgaca gcgtgctgtt cgacggcgcc gcccggctgg atttgccagt
      481 ggaaaaacgc ggcctgggcc tggtgtttca gtcctacgcg ctgtggccgc accgcacggt
      541 gttcgataac gttgcctacg gcctcaagct gcgcaaagtg gccgacgccg acatccgcca
      601 gcgggtggat caggcgctgg ccagcctgga cctgggccac ctggccgcgc gttttccgca
      661 ccaactctct ggcggccagc agcagcgcgt ggcgattgcc cgcgccctgg tgtacaaccc
      721 gccggtgatc ctgctggacg aacccttgtc caatctggac gccaagctgc gcgaagaagc
      781 gcgctcgtgg ctgcgcgagc tgattgtcac cctgggcctg tcggcgctgt gcgtcaccca
      841 cgaccagacc gaagccatgg ccatgtccga ccgcatcctg ctgctgaaag acggccgcat
      901 cgagcagcaa ggctcgccgc aagacatgta cgacaacccg gccagcctgt ttgtggcgga
      961 tttcatgggc aataacaacc ggctgaatgg ccagatcagc gaagtgcgcg atggccgcgc
     1021 caagatcagc ggcgagggct ggtcgttctg gggcagcctg cgcggcgacg cccagcccgg
     1081 ccgcgccgcc acggcgatga tccgcctgga gcgcgtgcgc gtggccgacg gccccggcga
     1141 caaccacatc gccctgccgc tggtc
//
LOCUS       DAOPYX010000475         1137 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_204806_length_1137_cov_4.377079, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000475 DAOPYX010000000
VERSION     DAOPYX010000475.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 1137)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1137)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1137
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_204806_length_1137_cov_4.377079"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            618..854
                     /locus_tag="PKN55_14605"
     CDS             618..854
                     /locus_tag="PKN55_14605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX72526.1"
                     /translation="MPRARDPSLSTRQYPLRVYLRDVVNCTTTHGVSYLAWCYVYHPI
                     ISIPHANLIPADEGGVSRFDPEVGGHHAAQRADF"
     gene            complement(902..>1137)
                     /locus_tag="PKN55_14610"
     CDS             complement(902..>1137)
                     /locus_tag="PKN55_14610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011134353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNX72527.1"
                     /translation="SAFSSIVVYAQELLPGRVGMVSGLMFGFAFGVGGVGAGALGQLA
                     DQYGVEAVYQWCAWLPLLGVCAMWLPDVRHGRA"
BASE COUNT          227 a          378 c          303 g          229 t
ORIGIN      
        1 aacccctcgt cgcaccacac cgcgtcattc ccgcgcaggc gggaatccag gtgtgtccac
       61 agcgtactgc ggtctaagct ccggcttgtc ggttcaaatc atggtgactg actactagcc
      121 agggccgctt cgctgggttt tgtgagcgac tctaagccgc ccgcctggtc atcatggccg
      181 cgcgggcggc ttttttgcgt gccgggcttg gcctgatcaa cgggatatgc catattgatg
      241 aaagttggcc tgaacaccgg ctacccaagc ccgtctggcc gtcgtccggt caggcattcg
      301 tatgtttgcc tttgttccat caatgaaact ccaccccgtc agactcccca tctttgtcta
      361 tggcacgcta aaaaccggtg gcctgtatca ccatctgatt gcgcaccaaa ttgaaaacac
      421 cgatgaagcc acgacccgtg gctcgctgta ttacaaaata ttcaactgca accaaaccgg
      481 ccgacgtgag gtgacagcgg cttattcgcc cgatggcccg tttccgattg ctggcacctt
      541 gttgtttccg cgcgccagcg agtattacag cctgctgtcg cggctggacg agctggaatt
      601 caattttgac gccagcaatg ccgcgcgcgc gcgaccccag cctgagcacc cggcaatatc
      661 cgctgcgcgt gtatctgcgc gacgtggtca actgcaccac cacccatggt gtgtcgtatc
      721 tggcctggtg ttatgtctac cacccgatta tctcgattcc gcacgccaac ctgatcccgg
      781 ctgacgaggg cggcgtctcc cgttttgatc cggaagtggg ggggcaccat gctgcacagc
      841 gggctgattt ttgaggatgg cttggtacgc ttgtaacacg cgaccaccaa cgcgcgctga
      901 ttcaggcgcg cccgtggcgt acatccggca gccacatcgc acacaccccc aacagcggca
      961 gccaggcgca ccactgatac accgcctcta caccgtactg gtcagccagt tggcccagtg
     1021 cgccagcgcc cacgccgccc acgccaaagg caaagccaaa catcagcccg gataccatgc
     1081 ctacccggcc gggcagcagc tcttgcgcat acaccacaat cgaagaaaac gccgagg
//
LOCUS       DAOPYX010000476         1119 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_210071_length_1119_cov_2.901316, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000476 DAOPYX010000000
VERSION     DAOPYX010000476.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 1119)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1119)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1119
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_210071_length_1119_cov_2.901316"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            91..>1119
                     /locus_tag="PKN55_14615"
     CDS             91..>1119
                     /locus_tag="PKN55_14615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX72528.1"
                     /translation="MNAQPAVRRYHAALWLSAILLLIWSIPHTVALRYTLMTSLALCV
                     FSLPAVRQAVWAQCRAQPVLWVYLALSVWLVIQIPTVAMHPQETFEELRGQWLKSSLA
                     LLLGMGVMQLGRVTPGAGRGVVFHWVFLALGAAVWMQGADYFWAWLLQGQSSFHSTGI
                     FNGKVQVSYLANLFLSLCMAEAFSRVCGGGRHVAIPSPLLLAWALCAVAVTFFSGARN
                     GLIGVLLLCVSTGLLMVCYAPARVSRAQRLGGFVLLLAVAGSLAGAQLKTDPRWMRFV
                     ESTSYSFQTDTVLSWLDPKRYPYPILSDGQPLDDSAYERTAWIIVGMKLSAEHKLGVG
                     FTRKAYTNT"
BASE COUNT          141 a          325 c          384 g          269 t
ORIGIN      
        1 cacgcgcgcc tgtattgaat cgatttccgc ccagcagatt gccgacgccg cgctggcctt
       61 gctggcggat gcccctcgga ccgccgccac gtgaatgccc agccggctgt gcgccgttac
      121 catgccgcgc tctggttgag cgccatcctg ttgttgatct ggtcgattcc ccatactgtg
      181 gccttgcgct acaccttgat gaccagcctg gcgctgtgcg tgttcagcct gccggcggtg
      241 cgtcaggcgg tgtgggcgca gtgccgcgcg cagcccgtct tgtgggtgta tctggccttg
      301 agtgtatggt tggtgatcca gattccgacg gtggccatgc acccgcagga aacctttgaa
      361 gaattgcgcg gccagtggct aaaaagctcg ctggccttgt tgctgggcat gggggtgatg
      421 cagctgggcc gcgtcacgcc gggcgctggg cgcggcgtgg tgtttcattg ggtgtttctg
      481 gccttgggcg cggcggtgtg gatgcagggc gcggattatt tctgggcctg gctgttgcaa
      541 ggtcagtctt cgtttcattc cactgggatt ttcaacggca aggtgcaggt gtcttacctg
      601 gccaatttgt tcttgtcgct gtgcatggcc gaggcgttca gccgggtgtg cggcggcggg
      661 cggcatgtgg cgattccttc gccgctcttg ctggcttggg cgctatgcgc ggtggcggtg
      721 acgttttttt ctggcgcgcg caacggcttg attggcgtgc tgctgctgtg tgtgtccacg
      781 ggtttgctga tggtgtgcta cgcgccggcg cgcgtcagcc gcgcgcagcg cttgggcggc
      841 tttgtgctgc tgctggccgt ggccggcagc ctggccggcg cgcagctgaa aaccgacccg
      901 cgctggatgc gctttgtgga gtctacgtcc tattctttcc agaccgacac cgtgttgagc
      961 tggctggatc ccaagcgcta cccttatccg attctgtctg atggccagcc gctggatgat
     1021 tcggcttacg agcgcaccgc ctggatcatc gtcggcatga agttatccgc cgagcacaaa
     1081 ctgggcgtgg gcttcacccg caaggcgtac accaacacg
//
LOCUS       DAOPYX010000477         1109 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_213281_length_1109_cov_1.828273, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000477 DAOPYX010000000
VERSION     DAOPYX010000477.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 1109)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1109)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1109
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_213281_length_1109_cov_1.828273"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..447)
                     /locus_tag="PKN55_14620"
     CDS             complement(<1..447)
                     /locus_tag="PKN55_14620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX72529.1"
                     /translation="MSVIIITGMTVIDTTTTGVMFRQAGSVRLAGMTIHLPVAANVKK
                     RGCAHRAGMTRLPDRVAECFFPVLLLLRQSVPQPGALRIGPALGLVHYSSGIFHPFPH
                     RYCPHVRRGLVTIVADRDIHECAGGNLPGPQTPDEPAPASKRVFMVG"
     gene            complement(758..>1109)
                     /locus_tag="PKN55_14625"
     CDS             complement(758..>1109)
                     /locus_tag="PKN55_14625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX72530.1"
                     /translation="QMPVAGELPMSICQTMRWTALCLLMTTSAAWATTPAAEQRVERI
                     EQRADMARQHANQAEARASRIEAASADARHPRLAQQRERRAERHARRVDHRADRVEGR
                     AERKISRIQSRGTQ"
BASE COUNT          220 a          336 c          359 g          194 t
ORIGIN      
        1 gcctaccatg aataccctct tgcttgccgg ggccggttcg tctggtgttt gtgggccagg
       61 gagattaccg ccggcacact cgtggatgtc acgatcagca acgattgtaa caagcccgcg
      121 cctgacgtgg gggcagtatc ggtggggaaa ggggtgaaaa atgcctgaag aataatgcac
      181 cagccccaac gcagggccaa tacgcaatgc gccaggctga ggcacagact ggcgcaaaag
      241 cagcaacacc gggaaaaaac actcagcgac gcgatccggc aggcgggtca taccagcccg
      301 gtgggcgcac ccacgcttct tcacgttcgc ggccacgggc agatggatgg tcattccagc
      361 caggcggacg ctgccagcct ggcggaacat cacgccggtc gtggtcgtat cgatcacggt
      421 catgccggtg atgatgataa cgctcacgcg atggcgcggg ataaaccgga taggcagggt
      481 acaccacagg cggcgatact tgatacaccg gataaaccgg ctggccaaga ataatctgca
      541 cccccagtgc cggggattgg gccaaggcgg caggggtggc cgccagcgcc agccacaggg
      601 tggtcagtcg aagaaagcgc atggcgtgac tccttgtccg gaaatggccg gaagcggtgg
      661 atgaatgggc tggtggagca ttttacccca ccctcaagct caacatagca ggatgccgca
      721 cccactgctt tgctaccgcc ctggcccggc aaatggctta ctgcgtaccc cggctttgaa
      781 tccggctgat tttacgctca gcgcgaccct ccacccgatc tgcccggtga tccacccgac
      841 gggcgtgtcg ctccgcgcgg cgttcgcgtt gctgtgccag gcgcggatga cgggcatctg
      901 cgctggcggc ttcgatccgg ctggcccgtg cctcggcctg attggcgtgc tgacgcgcca
      961 tgtctgcgcg ctgttcaatc cgctcaaccc gttgttcggc agcgggcgtg gtggcccagg
     1021 cggcagaggt ggtcatcagc agacacaatg cggtccagcg catggtttgg catatcgaca
     1081 tcgggagttc tccggcaaca ggcatctgg
//
LOCUS       DAOPYX010000478         1107 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_213688_length_1107_cov_9.852662, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000478 DAOPYX010000000
VERSION     DAOPYX010000478.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 1107)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1107)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1107
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_213688_length_1107_cov_9.852662"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            111..716
                     /locus_tag="PKN55_14630"
     CDS             111..716
                     /locus_tag="PKN55_14630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018152680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HNX72531.1"
                     /translation="MSGNLIWAMATFALAGAISPGPVNVLATAVGARAGFVRALPHVT
                     GASVCYVLIVWLSGTGLQQLWRGWPWLGGALPYAGAGYLLYLAARIALAPVRAEDDDS
                     AEAQSGLWRGALCQGLNPKAWWVAMSGVSVLVGVGEGQALRLMWFCALSGVICWLSVA
                     TWAGLGRVLRPWLAQPARQRVFNWLMAAALLAAVGPLLSPV"
     gene            752..>1107
                     /locus_tag="PKN55_14635"
     CDS             752..>1107
                     /locus_tag="PKN55_14635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018152679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HNX72532.1"
                     /translation="MVMIEADPHLSLQRSVALPWVDMRRANRSSACYQTHSHDEFSFG
                     VIDNGHARYLNHGRQHHIGPGMTVWIDPGVAHACNPALGAWSYRMLFVEAGWMGRLQE
                     ELPGLPHQDYTPFAAP"
BASE COUNT          138 a          320 c          418 g          231 t
ORIGIN      
        1 agccgcccga ccgccaaaca gcccggttct cgggctgcaa tttcgttcaa tacccgctcc
       61 cttgcctgtt tatacactgc ccgtcatctg gattgagaag ggggcggggt atgtcgggca
      121 atcttatttg ggcgatggcc acgtttgcgc tggctggggc gatttcgccg gggccggtga
      181 atgtgctggc cacggcggtg ggcgcgcggg ccgggtttgt gcgcgccttg ccgcatgtca
      241 ccggcgccag cgtgtgttat gtgttgatcg tctggctgtc cggcaccggt ttgcagcagc
      301 tgtggcgcgg ctggccgtgg ctgggcggcg ctttgccgta tgcgggagcc gggtatttgc
      361 tgtatctggc cgcgcgcatt gccctggcgc cggtgcgcgc agaggatgac gacagcgccg
      421 aggcgcagtc cgggctgtgg cgcggcgcgc tgtgccaggg gctgaaccca aaagcctggt
      481 gggtggcgat gtcgggcgtg agtgtgctgg tgggggtggg cgaaggccag gcgctgcggc
      541 tgatgtggtt ttgcgcgctg tccggggtga tttgctggct gtcggtggcc acctgggccg
      601 gcttgggccg ggtgttgcgg ccttggctgg cgcagccggc gcgccagcgg gtgtttaact
      661 ggctgatggc ggcggcgctg ttggcggcgg tggggccgtt gttgtcgccg gtgtgagcgc
      721 cgggcccgcc gtcacgtatg gatttgggag catggtgatg atcgaagccg accctcattt
      781 gagcctgcaa cgcagcgtag cgctgccgtg ggtggacatg cgccgcgcca accgctcgtc
      841 cgcctgctat caaacccaca gccatgacga gttttctttt ggcgtgattg acaatggcca
      901 cgcccgctat ctgaaccacg gccgtcagca ccatattggg ccaggcatga cggtgtggat
      961 cgaccccggc gtggcgcacg cctgcaatcc ggcgctgggc gcgtggtcgt atcgcatgct
     1021 gtttgtcgag gccggctgga tgggtcggct gcaagaagaa ttacccggcc tgccgcatca
     1081 ggactacacc ccgtttgccg cgccgca
//
LOCUS       DAOPYX010000479         1103 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_214945_length_1103_cov_8.622137, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000479 DAOPYX010000000
VERSION     DAOPYX010000479.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 1103)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1103)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1103
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_214945_length_1103_cov_8.622137"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            90..689
                     /locus_tag="PKN55_14640"
     CDS             90..689
                     /locus_tag="PKN55_14640"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNX72533.1"
                     /translation="MPHAQRGFTLVELAIALVIIGLVLGMTFKGRELIDGAKVKSVQA
                     QYGKISAAMNIFFEKYGFYPGDGCASSGVTVVSSCTGVKDITLSTAAEQSSFWSLLNS
                     SGILPLSERKNPIGAEWEVKTGDGIDPADADRFPASNTFMYALSVDSRMLCQLEKQMD
                     DLDPATGLIRSDASSYSPATVDCWAISTSPAKMAIRLLP"
     gene            complement(769..>1103)
                     /locus_tag="PKN55_14645"
     CDS             complement(769..>1103)
                     /locus_tag="PKN55_14645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012695998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HNX72534.1"
                     /translation="QKTLLNIEGLGRQLDPDLDLWQTAKPFLVKWMNEQIGWRGLLRN
                     LRDEAPQWASLLPAIPRRLNEALSADTRDVLIQGYVTLLREQKRQNMLLMALVALLAA
                     GLLGLWLR"
BASE COUNT          211 a          333 c          328 g          231 t
ORIGIN      
        1 cattcccgcg cccttgctat tgcatgcact ggtcagcgcc ggtcgcctgc cttgagtctt
       61 gtcttcaaga cattgaaaaa ggaaatttca tgcctcatgc tcaacgtggc tttaccctgg
      121 tggagctggc catcgcgctg gtgattattg gtctggtgtt ggggatgacg ttcaaggggc
      181 gcgagctgat tgacggcgcc aaagtcaaaa gcgtgcaggc gcagtacggc aaaatcagcg
      241 cggcaatgaa tatctttttt gagaaatacg gtttttatcc tggcgacggc tgcgccagca
      301 gcggcgtcac cgtggtcagc agctgcaccg gcgtgaagga catcaccttg agcaccgccg
      361 ctgagcagtc ttctttctgg agcttgctca atagctcggg catcttgccc ttgtccgagc
      421 gcaaaaaccc gattggcgca gaatgggaag tcaaaaccgg cgacggcatc gacccggccg
      481 acgctgaccg ctttccggcc agcaacacct ttatgtatgc gctgtcggtg gacagccgca
      541 tgttgtgtca gctggaaaag cagatggacg atcttgaccc ggccaccggg ctgattcgct
      601 ctgacgccag cagttattct cctgccacgg tggattgctg ggcgatcagc accagcccgg
      661 ccaagatggc gattcgcctg ctgccgtgac aaactgccgc catgtgtgga ggccggcgat
      721 tgtgctggcc agcgagggca tggcgcgcgt gtgccttcgg gggacttttc agcgcagcca
      781 cagccccagc aaaccggccg ccagcagcgc caccagcgcc atcagcagca tgttctggcg
      841 cttttgctcg cgcaatagcg tcacataacc ctgaatcaac acatcgcggg tgtccgctga
      901 cagcgcctcg ttcagccggc gcggaattgc cggcagcagg ctggcccatt gcggcgcctc
      961 gtcgcgcaga ttgcgcaaca gcccgcgcca gccgatttgc tcgttcatcc acttcaccaa
     1021 aaatggcttg gcggtttgcc acaggtccag atccgggtcg agctggcggc ccaggccttc
     1081 gatattcagc agggtttttt gca
//
LOCUS       DAOPYX010000480         1098 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_216704_length_1098_cov_2.712368, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000480 DAOPYX010000000
VERSION     DAOPYX010000480.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 1098)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1098)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1098
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_216704_length_1098_cov_2.712368"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..382)
                     /locus_tag="PKN55_14650"
     CDS             complement(<1..382)
                     /locus_tag="PKN55_14650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008954702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HNX72535.1"
                     /translation="MKFASLIRPTPSDRSPTPLTAHDDGLVWAIALLLSIGFVMVYSA
                     SIAYAEADPDTHNRYFYLIRHGVYLTISLIVSWIVFHIPSRIWQEKAPYLFVGAFILL
                     ILVLVPGIGKVVNGSRRWINLVVLN"
     gene            complement(379..954)
                     /locus_tag="PKN55_14655"
                     /pseudo
     CDS             complement(379..954)
                     /locus_tag="PKN55_14655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018151926.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
BASE COUNT          224 a          369 c          335 g          170 t
ORIGIN      
        1 aattcagcac caccagatta atccagcgcc gcgaaccatt caccaccttg ccgatgcccg
       61 gcaccagcac caaaatcagc agaataaacg cgcccacaaa cagatacggc gctttttcct
      121 gccagatccg gctgggaata tgaaacacaa tccagctgac aatcagcgag atggtcagat
      181 acacgccatg gcggatcaga taaaaatagc ggttatgggt atccgggtcg gcttcggcgt
      241 aggcaatcga cgcggaatac accatgacaa agccaatcga cagcagcagg gcaattgccc
      301 agaccagccc gtcatcgtgg gcagtcagcg gggtgggaga gcggtcggag ggggttggcc
      361 gaatcaggct ggcaaatttc acaggcgggc ctcgtcagcc agttgccggg cgctggcgat
      421 aaatacctcg gcgcggtggg catagttgcg gaacatgtcc aggctggcac aggccggcga
      481 cagcagcacc acatcgcccg gttgggcggc ctgatacgcc aggcgggcgg catcttccag
      541 ggtatcggcc cggtgtaccg gcacgctcac cgctgccagc gcggcttcaa tacgctcggc
      601 gtctcggcca atcagcagca ctgcgcgggc aatgcgcgcc accgccgctg ccagcggggt
      661 aaagtcctgg cccttgccat cgccgccggc aatcagcacc accggacggc tcatgccgtt
      721 cagcgcggct tcggtggccc ccacattggt gcctttggag tcgtcgtaaa aatccacccc
      781 gttcagctgc agcacatgct ccacccggtg tgccagcccc ttgaagcggc acagcccggc
      841 cagcaggctg gcacgatcca ggccgatggc ctcgcacagt gccagcgccg ccagtgcatt
      901 ggcggcgtta tgcaggccag tgagctgcat ctgggcaacg tccagcaccg gctcgccatc
      961 caccgccaag gcaatgctct ggccctcgcg gtacaggcca tactgccgcg ccatggttcg
     1021 tcctggccgg gccgtgcgcc gcttcagcct gacccagccg acgcagtatg gcctgaagcg
     1081 gcgcacggcc cggccagg
//
LOCUS       DAOPYX010000481         1090 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_219181_length_1090_cov_11.085024, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000481 DAOPYX010000000
VERSION     DAOPYX010000481.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 1090)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1090)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1090
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_219181_length_1090_cov_11.085024"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1029
                     /locus_tag="PKN55_14660"
     CDS             <1..1029
                     /locus_tag="PKN55_14660"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY family inner membrane protein"
                     /protein_id="HNX72536.1"
                     /translation="ARIKLFLLSNLVPDFAGKIISVYMRQFADNAGRLTAVGLALLAA
                     TALSLMLTIERTFNAIWRVHAPRPFVRQMLIYWAMLTVGPLLLGGGLSALGRLMQASA
                     LERQAPALADALQWLASLGLTTLVLMALYALTPNRHVPRKHALIGATVAALVLTLTQS
                     GFGVYVGLARGNPLVFGAVSAFPILLLWLQLIWLVVLSGAVLTAALPYWADEAWHDAS
                     GVSRRWLHVLDTLLCLSAAQERGRALTLGRLRDDVHTGYDELGQILDTLARAGLVSRT
                     RDDGWLLTGRPEHITLRQLHQLFVLPPLPERLRSPTAQALRATLAPGEAALDISLAEF
                     ARRCAVAG"
BASE COUNT          128 a          355 c          383 g          224 t
ORIGIN      
        1 gcgcgcatca agctgttttt gctgagcaat ctggtgccgg attttgccgg caagattatt
       61 tcggtgtata tgcgccagtt tgccgacaac gccggccggc tgaccgccgt gggcctggcg
      121 ctgttggccg ccaccgcgct gtcgctgatg ctgaccattg agcgcacctt taacgccatc
      181 tggcgggtgc atgcgccgcg tccgtttgtg cggcagatgt tgatttactg ggcgatgctc
      241 accgtcggcc ccttgctgct gggcggcggc ttgtcggcgc tgggccgcct gatgcaggcc
      301 agcgcgctgg agcgccaggc gccggccttg gccgacgcgc tgcaatggct ggcctcgctg
      361 gggctgacca cgctggtgct gatggcgctg tacgcgctca cccccaaccg ccatgtgccg
      421 cgcaagcacg ccttgattgg cgccaccgtg gcggcgttgg tgctgacgct gacgcaaagc
      481 ggctttggcg tgtatgtggg cctggcgcgc ggcaatccgc tggtgtttgg cgcggtgtcg
      541 gcgtttccga ttttattgct gtggctgcaa ttgatctggc tggtggtgtt gtccggcgcg
      601 gtgctgaccg ccgccctgcc ctactgggcg gacgaggcct ggcacgacgc cagcggcgtc
      661 agccggcgct ggctgcatgt gctggacact ctgctgtgcc tgagcgcggc gcaggaacgc
      721 ggccgggcct tgacgctggg ccggctgcgc gacgatgtgc acaccggcta cgacgagctg
      781 ggccagattc tcgacaccct ggcgcgcgcc ggcttggtca gccgcacccg tgacgatggc
      841 tggctgctga ccggccggcc cgagcacatc accctgcgcc agctgcatca gctgtttgtc
      901 ttgccgccgc tgcccgagcg gctgcgctcg cccactgcgc aggcgctgcg cgccaccttg
      961 gcgccaggcg aggcggcgct ggacatcagc ttggctgagt ttgcccgccg ttgtgcggtg
     1021 gcgggttaag cgccgctcac aaaaccccct ggcgttgttg tgtgaccttg ccgtacgact
     1081 tgtactgtct
//
LOCUS       DAOPYX010000482         1086 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_220518_length_1086_cov_5.849661, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000482 DAOPYX010000000
VERSION     DAOPYX010000482.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 1086)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1086)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1086
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_220518_length_1086_cov_5.849661"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            271..825
                     /locus_tag="PKN55_14665"
     CDS             271..825
                     /locus_tag="PKN55_14665"
                     /inference="COORDINATES: protein motif:HMM:NF017868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HNX72537.1"
                     /translation="MKLHPVTLPVFVYGTLKAGGLYHHLIAHQIEGSDEASTRGSLYY
                     KIFNCNQTGRREVTAAYSPDGPFPIAGTLLFPRASEYYSLLSRLDELEFNFDASNAAR
                     MRDPSLSTRQYPLRVYLRDVVNCTTAHGVSYLAWCYVYHPIISIPHANLIPADESGAT
                     CFDPEVGSAMLRNGLIFEDGLARL"
     gene            complement(851..>1086)
                     /locus_tag="PKN55_14670"
     CDS             complement(851..>1086)
                     /locus_tag="PKN55_14670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011134353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNX72538.1"
                     /translation="SAFSSIVVYAQELLPGRVGMVSGLMFGFAFGVGGIGAGALGQLA
                     DHYGVEAVYQWCAWLPLLGVCAMWLPDVRHERA"
BASE COUNT          194 a          362 c          302 g          228 t
ORIGIN      
        1 accctcctgg cgttgttgca cgaccttgcc gtactacccg tactgtctgc ggtcgcgcgc
       61 ctagccagga gcgctgcgct gggttttgtg agccgctcta agccgcccgc ctggccaatc
      121 atggccgtgc gggcggcttt tttgcggcgc cgggcttggc ctgagtgaat gtctatgcca
      181 tattgacgat ggttagcgtt ctacgctgtg gttctagaat aggcccgccc ggttgccggg
      241 cgggcatgac gtttttgccc ttgcccgccc atgaaacttc atcccgtcac cctgcctgtg
      301 tttgtgtatg gcaccttgaa agccggcggc ctgtatcacc atctgattgc gcatcagatt
      361 gaaggctccg atgaggcctc cacccggggt tcgttgtact acaaaatatt taactgcaat
      421 cagaccggcc gacgtgaggt gacggcggcg tattcgcccg atggcccgtt tcctatcgcc
      481 ggcaccttgc tctttccgcg cgccagcgaa tattacagcc tgctgtcacg gctggatgaa
      541 ctggagttca actttgacgc cagtaacgcc gcgcgcatgc gcgaccccag cctgagcacc
      601 cggcaatatc cgctgcgcgt gtatctgcgc gacgtggtca actgcaccac cgcccatggc
      661 gtgtcgtatc tggcctggtg ttatgtctat cacccgatta tctcgattcc gcacgccaat
      721 ctgattcccg ccgatgaaag cggcgccacc tgttttgacc cagaggttgg cagcgccatg
      781 ctgcgcaatg gcttgatttt tgaggatggt ctggcgcgct tgtaacccgc gatcaccaaa
      841 gcgcgctgag tcaggcgcgc tcgtggcgca catccggcag ccacattgcg cacaccccca
      901 gcagcggcag ccaggcacac cactggtata ccgcttccac gccgtaatgg tcggccagct
      961 ggcccagcgc gcctgcgccg atccctccca cgccaaaggc aaagccaaac atcagcccgg
     1021 acaccatccc cacccggccg ggcagcagct cttgcgcata caccacaatc gaagaaaacg
     1081 ccgagg
//
LOCUS       DAOPYX010000483         1049 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_233089_length_1049_cov_10.481891, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000483 DAOPYX010000000
VERSION     DAOPYX010000483.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 1049)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1049)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1049
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_233089_length_1049_cov_10.481891"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(69..401)
                     /locus_tag="PKN55_14675"
     CDS             complement(69..401)
                     /locus_tag="PKN55_14675"
                     /inference="COORDINATES: protein motif:HMM:NF024988.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HNX72539.1"
                     /translation="MEPLDPLLHQPLRTQLVAFLAGAGEQTFSELKRQLGVSDGNLDA
                     HLKKLLAADYVCVRKDASGARAQSFFALTPAGLTALQQYVQALQRLLPADLGGEAQSG
                     APAWVPAR"
     gene            complement(496..936)
                     /locus_tag="PKN55_14680"
     CDS             complement(496..936)
                     /locus_tag="PKN55_14680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX72540.1"
                     /translation="MMIRAASLLACATLLTACAGLGAPSSTDIQRLPVVRYGQAAPAD
                     RDFVLLYPAGADLPVNVQVDGSLLAKSDQAKLTVRVKRDVFVYRDQVSFDGKIWKRGQ
                     DCIGGNIVFSLPGDVPGSNGQARDGVSPGVLAARFDLRGEAAGK"
BASE COUNT          213 a          356 c          313 g          167 t
ORIGIN      
        1 cccgacacac ataaaaaaac cgccatccct gcaccggcag gcatggcggt tttttttgag
       61 gaggctgctc agcgcgccgg cacccatgcc ggcgcgccag actgcgcttc gccgcccaga
      121 tcggccggca gcaggcgttg cagcgcctgc acatactgtt gcagcgccgt caggccggcg
      181 ggcgtcagcg caaaaaacga ctgcgcgcgc gcaccactgg cgtctttgcg cacgcagaca
      241 tagtctgccg ccagcagttt tttcaggtgg gcgtccagat tgccgtccga cacgcccaac
      301 tggcgcttga gctcgctgaa agtttgctcg ccggcgccag ccagaaaagc caccagctgg
      361 gtgcgcaggg gttgatgaag caaagggtcg agaggttcca tactgcactt tctgcaatcc
      421 accagacagt atgcgcgcaa gcgcgccacc cggccagtgt ggggcggcgc tcaccctggc
      481 cagcgggatg ggcgcttact tgccggcggc ttcgccgcgc aaatcaaaac gcgccgccag
      541 cacccccgga ctgacgccgt cgcgcgcctg gccattgctg cccggcacat cgcccggcag
      601 cgagaacaca atattgccgc cgatgcagtc ctgaccgcgc ttccagattt tgccgtcaaa
      661 gctcacttga tcgcggtaga caaacacatc gcgcttcacc cgcacggtga gcttggcttg
      721 gtcgctcttg gccagcagcg agccatccac ctgcacattc accggcaaat ccgccccggc
      781 cggatacagc agcacaaaat cccggtcagc cggcgcggcc tggccgtagc gcaccaccgg
      841 caggcgttga atatcagtcg aggacggcgc gcccaggccg gcgcaggcgg tgagcagggt
      901 ggcgcaagcc agcagggaag cagcacggat catcatggtt attctcctaa attaggcact
      961 attgccgtca aaaagccagt tcacaggcgc acgcagccct ccggcgcgcc atgcggctgg
     1021 atatacacac aacacaggga tgttttaag
//
LOCUS       DAOPYX010000484         1026 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_241727_length_1026_cov_13.091658, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000484 DAOPYX010000000
VERSION     DAOPYX010000484.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 1026)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1026)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1026
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_241727_length_1026_cov_13.091658"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(81..923)
                     /locus_tag="PKN55_14685"
     CDS             complement(81..923)
                     /locus_tag="PKN55_14685"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019924660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HNX72541.1"
                     /translation="MFTTLISAAELAARAAGSVVLIDCRHQLTDTDYGRRAYAAGHVP
                     GAHFLHLDEDLSGPRTGDNGRHPLPDVGRLALTLGALGIGPDTQVVAYDDAGGMFAAR
                     AWWLLRWLGHEAVAVLDGGVQAWQAAGGALDAVTPARGNGGFLLRPTLEATLNADQLL
                     ALLGAPTLRLIDARAANRFAGQDETLDPVGGHIPGARNRFFQLNLDADGRFKPAAALR
                     AEWLACLDGQPAGAAIHQCGSGVTACHNLLALAHAGLGGGRLYPGSWSEWCANPARPV
                     ATGA"
BASE COUNT          183 a          351 c          362 g          130 t
ORIGIN      
        1 gtctgattga gaaacgctga cgattatgca gagcccgccg ggcccgggca agccgcgcag
       61 gcgggcgggg ctgacctgga tcaagcgccg gtagccaccg ggcgcgccgg gttggcgcac
      121 cactcgctcc acgaacccgg atacagccgg ccgccgccca ggccggcgtg cgccagcgcc
      181 aagagattat ggcaggcagt caccccggag ccgcactggt gaatcgccgc cccagccggc
      241 tggccgtcca ggcaggccag ccattcggcg cgcagcgccg ccgccggctt gaagcgcccg
      301 tcagcgtcca gattcagctg aaaaaaccgg ttgcgcgcgc caggaatatg cccgcccacc
      361 gggtccagcg tttcatcctg gccggcaaag cggttggcgg cgcgcgcgtc gatcaagcgc
      421 agcgtgggcg cgcccagcag ggccagcagt tgatcggcgt tcagcgtggc ctccagcgtc
      481 gggcgcagca gaaaaccgcc attgccgcgt gcgggtgtga cggcgtccag cgcgccgccc
      541 gcggcctgcc aggcctgcac gccgccatcc agcaccgcca ccgcctcatg gcccagccag
      601 cgcaacagcc accaggcgcg cgcggcaaac atgccgccag cgtcgtcata cgccaccact
      661 tgcgtgtccg ggccaatgcc caaggcgccc agggtcagcg ccagccggcc gacatcgggc
      721 aacggatggc ggccgttgtc gccggtgcgc gggccggaga ggtcttcgtc cagatgcaaa
      781 aaatgcgcgc caggcacatg cccggctgcg taagcgcgcc ggccgtagtc ggtgtcggtc
      841 agctggtggc ggcagtcgat cagcacaacg ctgccggccg cgcgggcggc cagctcggcg
      901 gcggaaatca gggtggtgaa catggcgaag actcctggtg cgacgggcaa gattgctggg
      961 tgattctagc tggcgggggc gaggatgaca aagtgatgtg ggtttacgca atatgccaaa
     1021 aaaaag
//
LOCUS       DAOPYX010000485         1024 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_242572_length_1024_cov_2.567595, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000485 DAOPYX010000000
VERSION     DAOPYX010000485.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 1024)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1024)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1024
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_242572_length_1024_cov_2.567595"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(32..826)
                     /locus_tag="PKN55_14690"
     CDS             complement(32..826)
                     /locus_tag="PKN55_14690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019924794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNX72542.1"
                     /translation="MNNALRAVWAFRGFVLTSVKREFQAKYRNSLLGATWTVIQPLSM
                     ILVYTVIFSHMMKSKLPGVEGGFAYSIYLCAGVLTWGLFVEIVSRAQTVFLDNANLIK
                     KLSFPKLCLPLIVVLNALLNFVIIFALFLGFLLAVGLFPGWVALAMLPVLLIQVMFAI
                     GLGIVLGVFNVFFRDVGQLFAVVINFWFWFTPIVYPANTLPAELQTWLTLNPMAALVG
                     AYQQIFVHGHAPDWASLAPVTVLAVVLCALGAQLFRRHAGEMVDEL"
BASE COUNT          261 a          337 c          275 g          151 t
ORIGIN      
        1 ttgcccaggc cgctcacttg aatcgcgccc atcagagttc atccaccatt tcgccggcat
       61 ggcgccggaa cagctgcgcg cccagcgcgc acaacaccac ggccagcacg gtcaccggcg
      121 ccaggctggc ccagtccggc gcatggccat ggacaaaaat ctgctggtaa gcgcccacca
      181 gcgccgccat cgggttgagc gtcagccagg tttgcagctc agccggcaag gtgttggcgg
      241 gatacacgat cggggtgaac cagaaccaga agttgatcac cacggcaaac agctggccca
      301 cgtcgcgaaa aaacacattg aacacgccca gcacaatgcc caggccaatg gcgaacatca
      361 cctgaatcag caacaccggc agcatcgcca gcgccaccca ccccggaaac aaacccaccg
      421 ccagcaaaaa gcccaaaaac agggcaaaga tgatgacgaa attcagcagg gcgttgagca
      481 ccacaatcag cggcaggcac agtttgggaa agctgagttt cttgatcagg ttggcgttgt
      541 ccagaaacac cgtttgcgcg cggctgacga tttcaacaaa caagccccag gtcagcacgc
      601 cggcgcacag atagatgctg taggcaaagc cgccttccac gccgggcagc ttggacttca
      661 tcatgtgcga gaaaatcacg gtgtacacca gaatcatcga caggggctga atcaccgtcc
      721 aggtcgcccc gagcagggaa ttacgatact tggcctggaa ctcccgcttg acgcttgtca
      781 ggacaaaacc cctgaacgcc cagaccgcac gcagtgcgtt attcatgacc tacattaacc
      841 tttgcgcacg ccgcagggcg aaagcgccaa aaccggctgt tgaacacagc ctgaaaaaat
      901 gacaggcagg gcgcccgccc cgatgccggc gcctgcgctc tgccctgtgt cgccgccgcc
      961 agacgcgggt cagctgcggc cataattatc ggcaaaacgc acaatgtcgt cctcgcccag
     1021 atac
//
LOCUS       DAOPYX010000486         1012 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_247119_length_1012_cov_6.410658, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000486 DAOPYX010000000
VERSION     DAOPYX010000486.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 1012)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1012)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1012
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_247119_length_1012_cov_6.410658"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(96..644)
                     /locus_tag="PKN55_14695"
     CDS             complement(96..644)
                     /locus_tag="PKN55_14695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX72543.1"
                     /translation="MQGPDDVVGALAYVLYKQQKIAFIEQIQRDQQREPNEQELQSFH
                     TISALPETLASYQQRAEALADQFLEIALAARLQQAETEIRQSTTLHMLESTKTDLTHV
                     VQASQALLTSRTDSILAELQGKKTIGGWLREVGGNLLVSVLTIVVVGALYTGYQWLSV
                     FNSNLEKHSGAAQHPAPPSARP"
     gene            complement(680..865)
                     /locus_tag="PKN55_14700"
     CDS             complement(680..865)
                     /locus_tag="PKN55_14700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX72544.1"
                     /translation="MTKFFSREDVLQANQRLMSKSTPLRDELSAASQKIVRDTRLSRQ
                     EINAAFKEARRRLTDAK"
BASE COUNT          205 a          269 c          293 g          245 t
ORIGIN      
        1 cactgcgtga aatggaagcc aaccgcccgc gcattcagct tgatttgccg ggggtagcgc
       61 cgttgaaggt gaagaagggc gggtgaggca aaacatcagg gacgggctga cggcggtgcg
      121 gggtgctgtg cggcgccgct gtgtttttcc agattgctgt tgaatacgct caaccactga
      181 tatccggtgt agagcgcccc cactaccacg atggtgagca cactgaccaa caaattaccg
      241 cccacttcgc gcaaccagcc gccgatggtt tttttgcctt gcagctcggc aagaatgcta
      301 tccgtcctgc tggtcaacaa agcctggctg gcttgcacga cgtgggtcaa atcggttttg
      361 gtggattcga gcatgtgcaa ggtggtggat tggcgaattt ctgtttcagc ctgttgcaaa
      421 cgggccgcga gcgctatttc cagaaattga tccgccagcg cttcggcgcg ctgctggtag
      481 ctggccaggg tttctggcaa ggcactgatc gtgtggaagc tctgcaattc ttgttcgttg
      541 ggctcgcgtt gctggtctcg ctgaatctgt tcaataaacg cgattttttg ctgtttgtag
      601 agcacatacg ccaaggcccc gacaacatca tcggggcctt gcaccagttt tctgtacacc
      661 cagttataag tttgcgattt catttggcgt ccgtcaggcg tcggcgcgcc tccttgaagg
      721 cggcattgat ctcttggcgg gacagccggg tgtcccgcac gatcttctgg ctggcggcgg
      781 acaattcatc gcgcaacgga gtggatttcg acatcaagcg ctggttggct tgcaggacgt
      841 cttcacgact gaaaaacttg gtcatgtgtt agataactcc tagacaatgc ctgcatttaa
      901 gcaaattttt acaagtaaaa caagcagatg ctggcaacgg tcatccatcc gtcagctcag
      961 cggatccacc tcgccaaacc gcgcatacac cgtcggcgag gcttccgggg tt
//
LOCUS       DAOPYX010000487         1006 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rivihabitans pingtungensis isolate AMR_MDS_1303
            NODE_249461_length_1006_cov_5.115668, whole genome shotgun
            sequence.
ACCESSION   DAOPYX010000487 DAOPYX010000000
VERSION     DAOPYX010000487.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854508
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rivihabitans pingtungensis (wastewater metagenome)
  ORGANISM  Rivihabitans pingtungensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Aquaspirillaceae; Rivihabitans.
REFERENCE   1  (bases 1 to 1006)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1006)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:03:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,941
            CDSs (total)                      :: 2,900
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1006
                     /organism="Rivihabitans pingtungensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_249461_length_1006_cov_5.115668"
                     /isolate="AMR_MDS_1303"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1054498"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1006
                     /locus_tag="PKN55_14705"
     CDS             <1..>1006
                     /locus_tag="PKN55_14705"
                     /EC_number="2.7.7.13"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR01479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="mannose-1-phosphate
                     guanylyltransferase/mannose-6-phosphate isomerase"
                     /protein_id="HNX72545.1"
                     /translation="RQGKLVTFGIVPTAPETGFGYIACGAADGHIRQVRGFVEKPSLE
                     VAQGYLASGDYLWNSGMFCFTAGAALAAFREHCPALFAAGEACWAASRGEGSATTEID
                     ADSFAGLEDISIDYAIMEKAANVAVVPASFDWSDIGSWNAMSQLVEPDENNNRAIGET
                     ITFASHGCYIHGDDRLIAALGVDDLIVVDTPDALLISHKDHTQDVKKVVQKLKTMGHD
                     SYRLHRTVSRPWGTYTVLEEGDRYKIKRIEVKPGASLSLQMHHHRNEHWIVVSGMARV
                     TNGEKGEFLVHTNESTYIPAGHKHRLENPGVIDLVMIEVQSGEYLGEDDIVRFADNYG
                     R"
BASE COUNT          212 a          299 c          313 g          182 t
ORIGIN      
        1 cccgtcaagg caagctggtg acctttggca ttgtgcccac cgcgccggaa accggttttg
       61 gctacatcgc ctgcggcgct gccgatggcc atatccgcca ggtgcgcggc tttgtggaaa
      121 aaccctcgct ggaagtcgcc caaggctatc tggccagcgg cgattatctg tggaattccg
      181 gcatgttctg ctttaccgct ggcgctgcgc tggcggcgtt ccgcgagcat tgcccggcgc
      241 tgtttgccgc gggcgaagcc tgctgggcgg ccagccgggg tgagggcagc gccaccaccg
      301 aaatcgacgc cgacagtttt gccgggctgg aagatatttc cattgattac gccatcatgg
      361 aaaaagccgc caatgtggcg gtggtgccgg ccagctttga ctggagcgac atcggctcgt
      421 ggaacgccat gagccagctg gtcgagccgg acgaaaacaa caaccgggcg attggtgaaa
      481 ccatcacctt tgccagccac ggctgttata tccacggcga cgaccgcctg attgccgcgc
      541 tgggtgtgga cgacctgatt gtggtggata ccccggatgc gctgttgatc agccacaagg
      601 accacacgca agacgtcaag aaagtggtgc aaaagctcaa gaccatgggt catgacagct
      661 accgcctgca ccgcaccgtg tcgcggccgt ggggcaccta caccgtgctg gaagaaggcg
      721 accgctacaa gatcaagcgc atcgaggtca aaccgggcgc gtccctgtcc ttgcagatgc
      781 accaccatcg caacgaacac tggattgtgg tcagcggcat ggcgcgcgtc accaatggcg
      841 aaaaggggga gttcctggtg cacaccaacg aatccaccta cattccggcc ggccataaac
      901 atcggctgga aaaccccggc gtgattgatc tggtgatgat cgaagtgcaa agcggcgagt
      961 acctgggcga agacgacatc gtgcgttttg ccgacaatta cggccg
//
